LOCUS       DAZPLI010000001        76174 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZPLI010000001 DAZPLI010000000
VERSION     DAZPLI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 76174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76174
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.001"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            50..373
                     /locus_tag="ACHUU3_000001"
     CDS             50..373
                     /locus_tag="ACHUU3_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR2080195.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(476..1726)
                     /gene="icd"
                     /locus_tag="ACHUU3_000002"
     CDS             complement(476..1726)
                     /gene="icd"
                     /locus_tag="ACHUU3_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR2080196.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1898..2551
                     /gene="rluE"
                     /locus_tag="ACHUU3_000003"
     CDS             1898..2551
                     /gene="rluE"
                     /locus_tag="ACHUU3_000003"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR2080197.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2561..3022
                     /gene="nudJ"
                     /locus_tag="ACHUU3_000004"
     CDS             2561..3022
                     /gene="nudJ"
                     /locus_tag="ACHUU3_000004"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR2080198.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            3076..4182
                     /gene="mnmA"
                     /locus_tag="ACHUU3_000005"
                     /gene_synonym="trmU"
     CDS             3076..4182
                     /gene="mnmA"
                     /locus_tag="ACHUU3_000005"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR2080199.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            4218..4859
                     /gene="hflD"
                     /locus_tag="ACHUU3_000006"
     CDS             4218..4859
                     /gene="hflD"
                     /locus_tag="ACHUU3_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR2080200.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4863..6233
                     /gene="purB"
                     /locus_tag="ACHUU3_000007"
     CDS             4863..6233
                     /gene="purB"
                     /locus_tag="ACHUU3_000007"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR2080201.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6401..7072
                     /gene="phoP"
                     /locus_tag="ACHUU3_000008"
     CDS             6401..7072
                     /gene="phoP"
                     /locus_tag="ACHUU3_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR2080202.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            7072..8532
                     /gene="phoQ"
                     /locus_tag="ACHUU3_000009"
     CDS             7072..8532
                     /gene="phoQ"
                     /locus_tag="ACHUU3_000009"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR2080203.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8608..9729
                     /gene="roxA"
                     /locus_tag="ACHUU3_000010"
                     /gene_synonym="ycfD"
     CDS             8608..9729
                     /gene="roxA"
                     /locus_tag="ACHUU3_000010"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR2080204.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9778..11004)
                     /gene="pepT"
                     /locus_tag="ACHUU3_000011"
     CDS             complement(9778..11004)
                     /gene="pepT"
                     /locus_tag="ACHUU3_000011"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR2080205.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            11254..12390
                     /gene="potA"
                     /locus_tag="ACHUU3_000012"
     CDS             11254..12390
                     /gene="potA"
                     /locus_tag="ACHUU3_000012"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR2080206.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12374..13231
                     /gene="potB"
                     /locus_tag="ACHUU3_000013"
     CDS             12374..13231
                     /gene="potB"
                     /locus_tag="ACHUU3_000013"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR2080207.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            13228..14022
                     /gene="potC"
                     /locus_tag="ACHUU3_000014"
     CDS             13228..14022
                     /gene="potC"
                     /locus_tag="ACHUU3_000014"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR2080208.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            14019..15065
                     /gene="potD"
                     /locus_tag="ACHUU3_000015"
     CDS             14019..15065
                     /gene="potD"
                     /locus_tag="ACHUU3_000015"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR2080209.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(15221..16042)
                     /gene="cobB"
                     /locus_tag="ACHUU3_000016"
     CDS             complement(15221..16042)
                     /gene="cobB"
                     /locus_tag="ACHUU3_000016"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR2080210.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(16058..16969)
                     /gene="nagK"
                     /locus_tag="ACHUU3_000017"
     CDS             complement(16058..16969)
                     /gene="nagK"
                     /locus_tag="ACHUU3_000017"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR2080211.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(16998..18242)
                     /gene="lolE"
                     /locus_tag="ACHUU3_000018"
     CDS             complement(16998..18242)
                     /gene="lolE"
                     /locus_tag="ACHUU3_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR2080212.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(18242..18943)
                     /gene="lolD"
                     /locus_tag="ACHUU3_000019"
     CDS             complement(18242..18943)
                     /gene="lolD"
                     /locus_tag="ACHUU3_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR2080213.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(18936..20135)
                     /gene="lolC"
                     /locus_tag="ACHUU3_000020"
     CDS             complement(18936..20135)
                     /gene="lolC"
                     /locus_tag="ACHUU3_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR2080214.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            20397..21470
                     /locus_tag="ACHUU3_000021"
     CDS             20397..21470
                     /locus_tag="ACHUU3_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR2080215.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            21598..25044
                     /gene="mfd"
                     /locus_tag="ACHUU3_000022"
     CDS             21598..25044
                     /gene="mfd"
                     /locus_tag="ACHUU3_000022"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR2080216.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            25188..26144
                     /gene="ldtC"
                     /locus_tag="ACHUU3_000023"
                     /pseudo
     CDS             25188..26144
                     /gene="ldtC"
                     /locus_tag="ACHUU3_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(26227..26484)
                     /gene="bhsA"
                     /locus_tag="ACHUU3_000024"
                     /gene_synonym="ycfR"
     CDS             complement(26227..26484)
                     /gene="bhsA"
                     /locus_tag="ACHUU3_000024"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFR2080217.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            26725..27357
                     /gene="comR"
                     /locus_tag="ACHUU3_000025"
     CDS             26725..27357
                     /gene="comR"
                     /locus_tag="ACHUU3_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR2080218.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(27419..27958)
                     /locus_tag="ACHUU3_000026"
     CDS             complement(27419..27958)
                     /locus_tag="ACHUU3_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR2080219.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(28185..29489)
                     /gene="ndh"
                     /locus_tag="ACHUU3_000027"
     CDS             complement(28185..29489)
                     /gene="ndh"
                     /locus_tag="ACHUU3_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR2080220.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(29897..30439)
                     /gene="ycfP"
                     /locus_tag="ACHUU3_000028"
     CDS             complement(29897..30439)
                     /gene="ycfP"
                     /locus_tag="ACHUU3_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR2080221.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(30462..31487)
                     /gene="nagZ"
                     /locus_tag="ACHUU3_000029"
     CDS             complement(30462..31487)
                     /gene="nagZ"
                     /locus_tag="ACHUU3_000029"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR2080222.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(31498..32322)
                     /gene="thiK"
                     /locus_tag="ACHUU3_000030"
     CDS             complement(31498..32322)
                     /gene="thiK"
                     /locus_tag="ACHUU3_000030"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR2080223.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(32303..32944)
                     /gene="lpoB"
                     /locus_tag="ACHUU3_000031"
     CDS             complement(32303..32944)
                     /gene="lpoB"
                     /locus_tag="ACHUU3_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR2080224.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(32958..33335)
                     /locus_tag="ACHUU3_000032"
     CDS             complement(32958..33335)
                     /locus_tag="ACHUU3_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR2080225.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(33338..33697)
                     /gene="hinT"
                     /locus_tag="ACHUU3_000033"
     CDS             complement(33338..33697)
                     /gene="hinT"
                     /locus_tag="ACHUU3_000033"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR2080226.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            34031..36220
                     /gene="fhuE"
                     /locus_tag="ACHUU3_000034"
     CDS             34031..36220
                     /gene="fhuE"
                     /locus_tag="ACHUU3_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFR2080227.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLVFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(36280..37713)
                     /gene="ptsG"
                     /locus_tag="ACHUU3_000035"
     CDS             complement(36280..37713)
                     /gene="ptsG"
                     /locus_tag="ACHUU3_000035"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR2080228.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(38008..38805)
                     /locus_tag="ACHUU3_000036"
     CDS             complement(38008..38805)
                     /locus_tag="ACHUU3_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR2080229.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(38816..39820)
                     /gene="holB"
                     /locus_tag="ACHUU3_000037"
     CDS             complement(38816..39820)
                     /gene="holB"
                     /locus_tag="ACHUU3_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR2080230.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(39817..40458)
                     /gene="tmk"
                     /locus_tag="ACHUU3_000038"
     CDS             complement(39817..40458)
                     /gene="tmk"
                     /locus_tag="ACHUU3_000038"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR2080231.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(40448..41470)
                     /gene="yceG"
                     /locus_tag="ACHUU3_000039"
     CDS             complement(40448..41470)
                     /gene="yceG"
                     /locus_tag="ACHUU3_000039"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR2080232.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(41473..42282)
                     /gene="pabC"
                     /locus_tag="ACHUU3_000040"
     CDS             complement(41473..42282)
                     /gene="pabC"
                     /locus_tag="ACHUU3_000040"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR2080233.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(42402..43643)
                     /gene="fabF"
                     /locus_tag="ACHUU3_000041"
     CDS             complement(42402..43643)
                     /gene="fabF"
                     /locus_tag="ACHUU3_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR2080234.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(43731..43967)
                     /gene="acpP"
                     /locus_tag="ACHUU3_000042"
     CDS             complement(43731..43967)
                     /gene="acpP"
                     /locus_tag="ACHUU3_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR2080235.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(44178..44912)
                     /gene="fabG"
                     /locus_tag="ACHUU3_000043"
     CDS             complement(44178..44912)
                     /gene="fabG"
                     /locus_tag="ACHUU3_000043"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR2080236.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(44925..45854)
                     /gene="fabD"
                     /locus_tag="ACHUU3_000044"
     CDS             complement(44925..45854)
                     /gene="fabD"
                     /locus_tag="ACHUU3_000044"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR2080237.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(45870..46823)
                     /gene="fabH"
                     /locus_tag="ACHUU3_000045"
     CDS             complement(45870..46823)
                     /gene="fabH"
                     /locus_tag="ACHUU3_000045"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR2080238.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIDKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(46891..47961)
                     /gene="plsX"
                     /locus_tag="ACHUU3_000046"
     CDS             complement(46891..47961)
                     /gene="plsX"
                     /locus_tag="ACHUU3_000046"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR2080239.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(48042..48215)
                     /gene="rpmF"
                     /locus_tag="ACHUU3_000047"
     CDS             complement(48042..48215)
                     /gene="rpmF"
                     /locus_tag="ACHUU3_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR2080240.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(48267..48788)
                     /gene="yceD"
                     /locus_tag="ACHUU3_000048"
     CDS             complement(48267..48788)
                     /gene="yceD"
                     /locus_tag="ACHUU3_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR2080241.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            48987..49571
                     /gene="yceF"
                     /locus_tag="ACHUU3_000049"
     CDS             48987..49571
                     /gene="yceF"
                     /locus_tag="ACHUU3_000049"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR2080242.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENACLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(49683..50642)
                     /gene="rluC"
                     /locus_tag="ACHUU3_000050"
     CDS             complement(49683..50642)
                     /gene="rluC"
                     /locus_tag="ACHUU3_000050"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR2080243.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            51215..54400
                     /gene="rne"
                     /locus_tag="ACHUU3_000051"
     CDS             51215..54400
                     /gene="rne"
                     /locus_tag="ACHUU3_000051"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR2080244.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(54595..55548)
                     /gene="flgL"
                     /locus_tag="ACHUU3_000052"
     CDS             complement(54595..55548)
                     /gene="flgL"
                     /locus_tag="ACHUU3_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFR2080245.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(55560..57203)
                     /gene="flgK"
                     /locus_tag="ACHUU3_000053"
     CDS             complement(55560..57203)
                     /gene="flgK"
                     /locus_tag="ACHUU3_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFR2080246.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(57269..58210)
                     /gene="flgJ"
                     /locus_tag="ACHUU3_000054"
     CDS             complement(57269..58210)
                     /gene="flgJ"
                     /locus_tag="ACHUU3_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR2080247.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(58210..59307)
                     /gene="flgI"
                     /locus_tag="ACHUU3_000055"
     CDS             complement(58210..59307)
                     /gene="flgI"
                     /locus_tag="ACHUU3_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR2080248.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(59319..60017)
                     /gene="flgH"
                     /locus_tag="ACHUU3_000056"
     CDS             complement(59319..60017)
                     /gene="flgH"
                     /locus_tag="ACHUU3_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR2080249.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(60070..60842)
                     /gene="flgG"
                     /locus_tag="ACHUU3_000057"
                     /pseudo
     CDS             complement(60070..60842)
                     /gene="flgG"
                     /locus_tag="ACHUU3_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(61014..61769)
                     /gene="flgF"
                     /locus_tag="ACHUU3_000058"
     CDS             complement(61014..61769)
                     /gene="flgF"
                     /locus_tag="ACHUU3_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFR2080250.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(61789..62994)
                     /gene="flgE"
                     /locus_tag="ACHUU3_000059"
     CDS             complement(61789..62994)
                     /gene="flgE"
                     /locus_tag="ACHUU3_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR2080251.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(63019..63714)
                     /gene="flgD"
                     /locus_tag="ACHUU3_000060"
     CDS             complement(63019..63714)
                     /gene="flgD"
                     /locus_tag="ACHUU3_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR2080252.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(63726..64130)
                     /gene="flgC"
                     /locus_tag="ACHUU3_000061"
     CDS             complement(63726..64130)
                     /gene="flgC"
                     /locus_tag="ACHUU3_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFR2080253.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNMVKSMMLKTLTLGQ"
     gene            complement(64134..64550)
                     /gene="flgB"
                     /locus_tag="ACHUU3_000062"
     CDS             complement(64134..64550)
                     /gene="flgB"
                     /locus_tag="ACHUU3_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR2080254.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            64705..65364
                     /gene="flgA"
                     /locus_tag="ACHUU3_000063"
     CDS             64705..65364
                     /gene="flgA"
                     /locus_tag="ACHUU3_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR2080255.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGNGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            65440..65733
                     /gene="flgM"
                     /locus_tag="ACHUU3_000064"
     CDS             65440..65733
                     /gene="flgM"
                     /locus_tag="ACHUU3_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR2080256.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            65738..66154
                     /gene="flgN"
                     /locus_tag="ACHUU3_000065"
     CDS             65738..66154
                     /gene="flgN"
                     /locus_tag="ACHUU3_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR2080257.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(66194..67729)
                     /gene="murJ"
                     /locus_tag="ACHUU3_000066"
     CDS             complement(66194..67729)
                     /gene="murJ"
                     /locus_tag="ACHUU3_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR2080258.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(67839..68762)
                     /locus_tag="ACHUU3_000067"
     CDS             complement(67839..68762)
                     /locus_tag="ACHUU3_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR2080259.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(68764..69411)
                     /locus_tag="ACHUU3_000068"
     CDS             complement(68764..69411)
                     /locus_tag="ACHUU3_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFR2080260.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(69422..70006)
                     /gene="rimJ"
                     /locus_tag="ACHUU3_000069"
     CDS             complement(69422..70006)
                     /gene="rimJ"
                     /locus_tag="ACHUU3_000069"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR2080261.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            70242..71450
                     /gene="mdtH"
                     /locus_tag="ACHUU3_000070"
     CDS             70242..71450
                     /gene="mdtH"
                     /locus_tag="ACHUU3_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR2080262.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            71514..72160
                     /gene="grxB"
                     /locus_tag="ACHUU3_000071"
                     /pseudo
     CDS             71514..72160
                     /gene="grxB"
                     /locus_tag="ACHUU3_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
     gene            72294..72854
                     /locus_tag="ACHUU3_000072"
     CDS             72294..72854
                     /locus_tag="ACHUU3_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR2080263.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            72960..74006
                     /gene="pyrC"
                     /locus_tag="ACHUU3_000073"
     CDS             72960..74006
                     /gene="pyrC"
                     /locus_tag="ACHUU3_000073"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR2080264.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLITCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            74080..74325
                     /gene="dinI"
                     /locus_tag="ACHUU3_000074"
     CDS             74080..74325
                     /gene="dinI"
                     /locus_tag="ACHUU3_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR2080265.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            74615..74869
                     /gene="bssS"
                     /locus_tag="ACHUU3_000075"
     CDS             74615..74869
                     /gene="bssS"
                     /locus_tag="ACHUU3_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR2080266.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            74984..76102
                     /gene="solA"
                     /locus_tag="ACHUU3_000076"
     CDS             74984..76102
                     /gene="solA"
                     /locus_tag="ACHUU3_000076"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR2080267.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPEQPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        18560 a        20229 c        19026 g        18359 t
ORIGIN      
        1 tgaacatcca aatgaactat attaaatacc gtgggataag acataacaaa tgaagtgggt
       61 agtaattgac acggtaattc aacctacatg cggcatatct ttttcagcca tatggggtga
      121 tatgaaaatg atcatctggt atcaatctac tatatttctt cctcctggaa gtatatttac
      181 accggttaag cttggtatta tccttaagga aaaagaatat cctattacta tttataacat
      241 tgcaccattc aacaagaatt tatggagttt actcaaaagc agtcaagagt gtcctccagg
      301 agaaagcaaa ataacaaata aatgtttaca taatagttgc attataaaaa tatgcccata
      361 tgggctcaag taatgggctt catataaata tgctcgatat gctattaata atatccacat
      421 aaataaaaca acgggcgtgt tatacgcccg tttcaatatt taactcatgg agagattaca
      481 tgttttcgat gatcgcttca ccaaactctg aacatttcag cagcttagcg ccttccatca
      541 gacgttcaaa gtcataagtt acggttttcg cgttgatagc accttccata cctttaacaa
      601 ttaaatcagc cgcttcagtc caccccatat ggcgcagcat catctcagcg gagaggatga
      661 tagagcctgg atttactttg tcctgaccgg catatttcgg cgcagtaccg tgggtggctt
      721 caaacagggc gcattcgtca ccgatgtttg cgccaggggc gataccgata ccgccaacct
      781 gcgctgccag ggcgtcagaa atgtagtcac cgttcaggtt catacaggcg ataacatcat
      841 attcagccgg acgcagcagg atctgttgca ggaatgcatc agcaatcacg tctttaatga
      901 cgatctcttt gccggtgttc gggtttttaa ctttcagcca cgggccgccg tcgatcagtt
      961 caccgccaaa ctcttcacgc gccagctggt agccccagtc tttaaacgcg ccttcggtga
     1021 acttcatgat gttgcctttg tgcaccagag tcacagagtc acgatcgtta gcaattgcgt
     1081 attcgatcgc tgcacgaacc agacgtttgg tgccttcttc cgaacacggc ttaataccga
     1141 taccacaatg ttccgggaag cgaattttct tcacccccat ctcttcacgc aggaatttaa
     1201 tcactttctc ggcgtcggca gagtctgctt tccattcgat acccgcataa atgtcttccg
     1261 agttttcacg gaagataacc atatcggtca gttcagggtg tttaaccggg cttggagtgc
     1321 cctgatagta acgtaccgga cgcaggcaga tgtagagatc cagttcctgg cgcagggcaa
     1381 cgttcagaga gcgaataccg ccaccaacag gagtggtcag cggaccttta atggcaacgc
     1441 gatattcacg aatcagatca agggtttcag caggcagcca gacatcctga ccataaacct
     1501 gtgtggattt ttcaccggtg taaatttcca tccaggagat tttacgctcg cctttatagg
     1561 ctttctcgac tgcagcgtcg accactttca gcatggctgg ggttacatct acaccgattc
     1621 catcaccttc aatgtaaggg ataatcggat tttcaggaac gttgagtttg ccgttttgca
     1681 gggtgatctt cttgccttgt gccggaacaa ctactttact ttccattcac ctctccttcg
     1741 agcgctactg gtttgctcgt ctgccaccac gttgcatatg cgtttgcgtc ctgcgatacg
     1801 gatgctttag agcaattttt tgttaatgat ttgtaattgg cttgtcaatg ctaccctatt
     1861 gtttgccgtc atgaaagatg cgcgttatta ggctataatg cggcaattca taatctctga
     1921 aaataccatg caaaaaactt cttttagaaa tcaccaggtt aagcgattca gctcgcaacg
     1981 ttctactagg cgtaaacctg aaaaccagcc cacgcgtgtg atcctgttca ataaacccta
     2041 cgatgttctt ccgcagttca ccgatgaagc cggacgcaaa acattaaaag aattcatccc
     2101 ggttcagggc gtttatgcag caggtcgtct tgaccgcgat agcgaagggt tactggtgct
     2161 gaccaataac ggcgcgttgc aggcgcgttt aacccagccg ggcaaacgca ccgggaaaat
     2221 ctattatgtg caggtggaag gtattcccac acaagacgca cttgaagcct tgcgcaatgg
     2281 cgtaacctta aatgatggcc ctaccctgcc cgccggcgcg gagctggttg acgaaccagc
     2341 gtggttatgg ccgcggaatc caccaattcg tgaacgcaaa agtattccca ccagctggct
     2401 gaagatcacc ttatatgaag gacgtaatcg ccaggtgcgc cgcatgaccg cccatgttgg
     2461 tttccccacg ctgcgactga ttcgttatgc gatgggtgat tacactttgg ataaccttgc
     2521 caatggcgaa tggcgagaag caacagaata aggatgcaca atgtttaaac cgcacgttac
     2581 cgttgcttgc gtagtgcacg cagagggtaa atttttagtc gttgaagaga cgattaatgg
     2641 taaagcgtta tggaaccaac ctgccgggca tctggaagcc gatgaaacct tagtggaagc
     2701 tgccgcccgt gagctatggg aagaaaccgg catcagcgcg caaccgcaac actttattcg
     2761 tatgcatcag tggattgcgc cagataaaac gccgtttttg cgtttcctct ttgccattga
     2821 gcttgagcaa atatgcccga cccagcctca tgacagcgat atcgactgct gtcgttgggt
     2881 cagcgccgaa gaaattttaa aggcgtcaaa tcttcgttcg ccgctggtgg cggaaagtat
     2941 ccgttgttat caaagtgggc aacgctatcc gctggagatg attggcgatt ttaactggcc
     3001 ttttacaaag ggtgtcatct aaaaggctgc gtgatagaat acgccgcctt gaagttcaat
     3061 gtcgtgagtg atccaatgtc tgaaaccgca aaaaaagtaa tcgtcggcat gtccggcggt
     3121 gtcgattcct ccgtttctgc ctggctgttg caacaacagg gatatcaggt tgaaggcctg
     3181 tttatgaaga actgggaaga agacgacggt gaggaatatt gcacagcggc agcggatctg
     3241 gctgatgccc aggctgtctg cgacaagctc ggcattgaac tgcacaccgt taactttgct
     3301 gccgagtact gggacaatgt cttcgaactg ttccttgccg aatataaagc cggtcgcacg
     3361 ccgaatccgg atattctgtg caacaaagag atcaaattta aagctttcct cgaatttgcc
     3421 gccgaagatt taggtgccga ttatatcgct accggtcatt acgtacgtcg ggccgatgtc
     3481 gatggcaaga gccgcctgct gcgtggtctg gacagcaata aagaccagag ctacttcctt
     3541 tatacgctca gccatgagca gattgcgcaa agcctgttcc cggtcggcga actggaaaaa
     3601 ccgcaggtgc gtaagattgc tgaagatctt ggtctggtca ccgcgaagaa aaaagactct
     3661 accggcattt gcttcattgg cgaacgtaaa ttccgcgagt tcctgggccg ttatctcccg
     3721 gggcaaccgg gcaaaatcat taccgtcgat ggcgatgaaa ttggtgagca ccaggggctg
     3781 atgtatcaca ctctcggtca gcgtaaaggt ctgggtatcg gtggcaccaa agaaggcacc
     3841 gaagaacctt ggtatgtggt ggacaaagac gtcgaaaaca acattctggt tgtcgctcag
     3901 ggccatgaac acccgcggct gatgtctgtc gggttgattg cccagcagtt gcactgggtc
     3961 gatcgcgaac cattcaccgg cactatgcgt tgcacggtaa aaacccgcta tcgccagacc
     4021 gatatccctt gcaccgtcaa ggcgctggac gatgatcgca ttgaagtgat tttcgatgaa
     4081 ccggttgccg ccgtgacgcc gggccagtct gccgtcttct ataacggtga agtgtgcctc
     4141 ggtggcggta ttattgagca gcgtctgccg ctgccggtct gattattatc tttacttaaa
     4201 acaaggaagc agtgaacgtg gcaaagaatt actatgacat caccctcgcc ctggccggta
     4261 tttgtcagtc ggcacgcctg gtgcaacaac tcgctcacca ggggcattgt gatgccgatg
     4321 cgctacacgt ctcactcaac agtattattg atatgaaccc cagctcgacg ctggcggttt
     4381 ttggcggtag cgaagccaac ctgcgcgtcg ggctggaaac cctgctcggc gtgctcaatg
     4441 ccagcagtcg ccagggctta aacgccgaat taacccgcta cacactcagc ttgatggtgc
     4501 ttgagcgcaa actctcctca gcgaaaggcg cgctcgacac tctgggcaac cggatcaacg
     4561 gcctgcaacg ccagctcgaa cacttcgatt tacagtccga aacgctgatg agcgcgatgg
     4621 ctgctatcta tgttgatgtg atcagcccgc ttggcccacg cattcaggtc accggttccc
     4681 ctgctgtact gcaaagccca caagtgcagg cgaaagttcg cgcaaccctg ctggcaggca
     4741 ttcgcgccgc cgtgctctgg caccaggtcg gcggcggacg tctgcaactg atgttttctc
     4801 gtaatcgcct gaccactcag gcaaaacaaa ttcttgctca tttaaccccg gagttgtgat
     4861 ctatggaatt atcctcactg accgccgttt cccctgtcga tggacgctac ggcgataaag
     4921 tcagcgcgct gcgcgggatt ttcagcgaat atggtttgct gaaattccgt gtacaagttg
     4981 aagtacgttg gctgcaaaaa ctggccgcgc acgcagcgat caaggaagtt cctgcttttg
     5041 ctgccgacgc aatcggttac cttgatgcaa ttgtcgccaa tttcagcgaa gaagatgccg
     5101 cacgcatcaa aaccatcgag cgtaccacta accacgacgt taaagcggtt gagtatttcc
     5161 tgaaagaaaa agtggcggag atcccggaac tgcacgcggt ttctgaattc atccactttg
     5221 cctgtacttc ggaagatatc aataacctct cccacgcatt aatgctgaaa accgcgcgtg
     5281 atgaagtgat cctgccgtac tggcgtcaac tgattgatgg cattaaagat ctcgccgttc
     5341 agtaccgcga tatcccgctg ctgtctcgta cccacggtca gccagccacg ccgtcaacca
     5401 tcggtaaaga gatggcaaac gtcgcctacc gtatggagcg ccagtaccgc cagcttaacc
     5461 aggtggagat cctcggcaaa atcaacggcg cggtcggtaa ctataacgcc cacatcgccg
     5521 cttacccgga agttgactgg catcagttca gcgaagagtt cgtcacctcg ctgggtattc
     5581 aatggaaccc gtacactacc cagattgaac cgcacgacta catcgccgag ctgtttgatt
     5641 gcgttgcacg cttcaacacc attctgatcg actttgatcg tgacgtctgg ggttatatcg
     5701 cccttaacca cttcaaacag aaaaccattg ctggtgagat tggttcttcc accatgccgc
     5761 ataaagttaa cccgatcgac ttcgagaact ccgaagggaa tctgggcctt tccaacgcgg
     5821 tattacagca tctggcaagc aaactgccgg tttcccgctg gcagcgtgac ctgaccgact
     5881 ccaccgtgct gcgtaacctc ggcgtgggta tcggttatgc gctgattgcg tatcaatcca
     5941 ccctgaaagg cgtgagcaaa ctggaagtga accgtgacca tctgctggat gagctggatc
     6001 acaactggga agtgctggct gagccaatcc agacagttat gcgtcgctat ggcatcgaaa
     6061 aaccgtacga gaagctgaaa gagctgactc gtggtaagcg cgttgacgcc gaaggcatga
     6121 agcagtttat cgacggtctg gcgttgccag aagaagagaa agcccgcctg aaagcgatga
     6181 cgccggcaaa ctacattggt cgcgccatca ccatggttga tgagctgaaa taaactcgta
     6241 tcagtgccgg atggcgatgc tgtccggcct gcttattaag attatccgct ttttattttt
     6301 tcactttacc tcccctcccc gctggtttat ttaatgttta cccccataac cacataatcg
     6361 cgttacacta ttttaataat taagacaggg agaaataaaa atgcgcgtac tggttgttga
     6421 agacaatgcg ttgttacgtc accaccttaa agttcagatt caggatgctg gtcatcaggt
     6481 cgatgatgca gaagatgcca aagaagccga ttattatctc aatgaacatt taccggatat
     6541 tgcgattgtc gatctcggat tgccagacga ggacggtctg tcactgattc gccgctggcg
     6601 tagcaatgat gtttcactgc cgattctggt attaaccgcc cgtgaaagct ggcaggacaa
     6661 agtcgaagta ttaagtgctg gtgctgatga ttacgtgact aaaccatttc atattgaaga
     6721 ggtgatggcg cgaatgcagg cattaatgcg gcgtaatagc ggtctggctt cacaggtcat
     6781 ttcgctacca ccgtttcagg ttgatctctc tcgccgtgaa ttatctatta atgacgaagt
     6841 gatcaaactg accgcgttcg aatacaccat tatggaaacg ttgatacgca ataatggcaa
     6901 agtggtgagc aaagattcgt taatgctcca actctatccg gatgcggagc tgcgggaaag
     6961 ccataccatt gatgtactga tgggacgtct gcgcaaaaaa attcaggcac aatatccgca
     7021 agaagtgatt actaccgttc gcggccaggg ctatttgttc gaattgcgct gatgaaaaaa
     7081 ttactgcgtc tttttttccc gctctcgctg cgggtacgtt ttctgttggc aacggctgcg
     7141 gtagtactgg tgctttcgct tgcctacgga atggtcgcgc tgatcggtta tagcgtcagt
     7201 ttcgataaaa ctacgtttcg gctgttacgt ggcgagagca atctgttcta tacccttgcg
     7261 aagtgggaaa acaataagtt gcatgtcgag ttacccgaaa atatcgacaa gcaaagcccc
     7321 accatgacgc taatttatga tgagaacggg cagcttttat gggcgcaacg tgacgtgccc
     7381 tggctgatga agatgatcca gcctgactgg ctgaaatcga atggttttca tgaaattgaa
     7441 gcggatgtta acgataccag cctcttgctg agtggagatc attcgataca gcaacagttg
     7501 caggaagtgc gggaagatga tgacgacgcg gagatgaccc actcggtggc agtaaacgtc
     7561 tacccggcaa catcgcggat gccaaaatta accattgtgg tggtggatac cattccggtg
     7621 gagctaaaaa gttcctatat ggtctggagc tggtttatct atgtgctctc agccaatctg
     7681 ctgttagtga tcccgctgct gtgggtcgcc gcctggtgga gtttacgccc catcgaagcc
     7741 ctggcaaaag aagtccgcga actggaagaa cataaccgcg aattgctcaa tccagccaca
     7801 acgcgagaac tgaccagtct ggtacgaaac ctgaaccgat tgttaaaaag tgaacgcgaa
     7861 cgttacgaca aataccgtac gacgctcacc gacctgaccc atagtctgaa aacgccactg
     7921 gcggtgctgc aaagtacgct gcgttctctg cgtagtgaaa agatgagcgt cagtgatgct
     7981 gagccggtaa tgctggagca aatcagccgc atttcacagc aaattggcta ctacctgcat
     8041 cgtgccagta tgcgcggcgg gacattgctc agccgcgagc tgcatccggt cgccccactg
     8101 ctggacaatc tcacctcagc gctgaacaaa gtgtatcaac gcaaaggggt caatatctct
     8161 ctcgatattt cgccagagat cagctttgtc ggtgagcaga acgattttgt cgaggtgatg
     8221 ggcaacgtgc tggataatgc ctgtaaatat tgcctcgagt ttgtcgaaat ttctgcaagg
     8281 caaaccgacg agcatctcta tattgtggtc gaggatgatg gccccggtat tccattaagc
     8341 aagcgagagg tcattttcga ccgtggtcaa cgggttgata ctttacgccc tgggcaaggt
     8401 gtagggctgg cggtagcccg cgaaatcacc gagcaatatg agggtaaaat cgtcgccgga
     8461 gagagcatgc tgggcggtgc gcggatggag gtgatttttg gtcgccagca ttctgcgccg
     8521 aaagatgaat aaatatgtcc atacttcacg cattacgtta agcatccgtt ataatcggtt
     8581 gcagatacca gcctgtggat gcttaacatg gaataccaac tcactcttaa ctggcccgat
     8641 tttcttgaac gtcactggca gaaacgcccg gtggtgttaa aacgcggctt taataatttt
     8701 attgacccga tctctccaga cgagttggcg ggtctggcga tggaaagcga agttgacagt
     8761 cgactggtca gtcaccagga tggcaaatgg caggtcagcc acggcccgtt cgaaagctac
     8821 gatcatctcg gtgaaaccaa ctggtcatta ctggtacagg cagtgaacca ctggcatgag
     8881 ccgaccgccg cgctgatgcg accgttccgt gaactaccgg actggcgtat tgatgatctg
     8941 atgatttctt tttctgtacc tggcggcggc gtcggcccgc atctcgatca gtacgacgtg
     9001 tttatcattc agggtaccgg acgtcgtcgc tggcgagtgg gcgaaaagct gcaaatgaaa
     9061 cagcactgcc cacatccgga tctgttacag gtcgatccgt tcgaagccat catcgatgaa
     9121 gagctggagc ctggtgatat tctttatatt ccgccaggat tcccgcatga aggctacgcg
     9181 ctggaaaatg cgatgaacta ttccgtgggc tttcgcgcgc caaatacgcg ggaactgatt
     9241 agtggatttg ccgattatgt gctgcaacgt gaactgggcg gcaactacta cagcgatcct
     9301 gatgttccac ctcgcgctca tcctgcggat gttctgcctc aagagatgga taaactgcgt
     9361 gagatgatgc tcgaattgat caaccagccg gaacacttta agcaatggtt tggcgagttt
     9421 atatcccagt cacgtcatga actggatatc gcgccgccag aaccgcctta tcagccagat
     9481 gaaatctacg atgcgctgaa acaaggtgat gtgctggtgc gcctgggtgg tctgcgcgta
     9541 ttgcgcattg gcgacgacgt gtatgccaat ggtgagaaga tcgattcccc gcaccgtccg
     9601 gcactggatg cactcgccag caacattgcg ctgactgcgg agaattttgg cgatgcgctg
     9661 gaagatccgt cattcctcgc gatgctcgcg gcgctggtca atagcgggta ttggttcttc
     9721 gaagggtaag tttggattta agccggatgc ggcatcacat gccgcatccc ttttcgctta
     9781 cttccgttgc gccgttaact cggcaatacg gacgatcacc tgcaccgctt tttccatacc
     9841 ttccagagtc acaaactcat gcttaccatg atagttgtaa ccgccagtga acaagttcgg
     9901 gcacggtaat cccataaacg acaactgcgc gccgtcggta ccaccgcgga tcggtttcag
     9961 ttccggttca atatcgcagt cacgcatcgc ctgctgggcg atatcgagaa tatgcggatg
    10021 ctcaaccact ttctctcgca tattgtagta actgtcttca atcaccagtt caatgtagca
    10081 atcaggatgt aaccctttgc ccactttttt ggcgatctcc atcattttac gtttacgcgc
    10141 ttcaaactgt ttacggtcga aatcacggat gatgtagtgc atatcggccc gttcaacggt
    10201 gcctttcatg ctcgccaggt gatagaaacc ttcatagcct tctgtcattt ccgggctttc
    10261 atccgccgga acttccgcat gaatacgtgc cgccagcgac agcgcattta ccatcactcc
    10321 tttcgccgtg cccggatgaa cattgttacc gacaattttg atattgaccg atgcggcgtt
    10381 gaagttttca aactccagtt cgcctacgcc accaccgtca acagtgtaag cccagcgggc
    10441 atcgaaggca tcaacatcaa aatgtttcgc ccctttgccc acttcttcat ccggggtaaa
    10501 ggcgacgcga atatcaccat gcggaatgtt tttctgttgc aataccgcca gcgcggtcat
    10561 gatttctgca atacctgctt tgtcatcggc acctaacaag gttttaccat cggtggtaat
    10621 caacgtctga cccagtagct gatgcagcac cgggaacata accggtgata aaacttcatc
    10681 gccgataccc agcgcaatat cgccaccgcg atagttttca acaatttgcg gattaacatt
    10741 tttgccgctg caatccggtg aggtatccac atgagaaata aagccaatcg ccgggatatc
    10801 gccagggacg ttagccggta acgtcgccat caaagtgccc ttctcactta aggtcacatt
    10861 gataagcccc atttcttcga gctgctcttt cagcagatgc aataacttcc attggccttc
    10921 cgtgctggga acctgtctca cccctgcttt tgattgggta tccagagata cgtagttcaa
    10981 aaatcgctca agtagtttat ccatgtagtc accctcactt tttgtgacaa cattattaag
    11041 aagcaagaaa agacaaatat tgcgtcaggt cacttttacc cctgcaagcg ggaatattta
    11101 tcagcattac cttaatgaat ataaagctaa gccagtaatt cacaacaaag attggcgatt
    11161 caagcggttt gccgtagaat taccgccctc attaagagtc accaaggtgg ttaaccacaa
    11221 accccgcatc ggtaagccat ccgttgcgtt tacatgggac agagtaaaaa attgaataaa
    11281 caaccgagtt cgctttcacc gctggtgcaa ttggcgggaa ttcgcaaatg ctttgatggt
    11341 aaagaggtca ttccccagct ggatctgact atcaacaatg gcgagttcct cacgctgctt
    11401 ggcccttctg gctgcggtaa aacaaccgtt cttcgcctga ttgcaggtct ggaaactgtt
    11461 gattccggac gcatcatgct ggataacgag gacatcaccc acgttccggc ggaaaaccgc
    11521 tatgtgaaca ctgttttcca aagctacgca cttttccccc acatgaccgt gttcgaaaat
    11581 gtggcctttg ggttgcgcat gcaaaaaacc cccgctgctg aaattacgcc ccgcgtgatg
    11641 gaagccctgc ggatggtgca actggaaacc ttcgctcaac gtaaaccgca tcagctctct
    11701 ggtggtcaac agcaacgcgt cgccattgct cgcgcggtgg ttaacaagcc tcgtctgttg
    11761 ttgctggatg agtcgctctc agcgctggat tacaaactgc gtaagcaaat gcagaacgag
    11821 ctgaaagcgt tacagcgtaa gcttggtatt accttcgtct ttgtcactca cgatcaggaa
    11881 gaagcgctca ccatgtcaga caggattgtg gtgatgcgcg agggtcgtat tgagcaagat
    11941 ggtacaccgc gcgaaattta cgaagagccg aaaaacctgt ttgttgccgg cttcattggc
    12001 gaaatcaata tgtttaacgc cactgtcatc gaacgtctcg acgagcagcg cgtacgcgcc
    12061 aacgttgaag gccgcgaatg taatatctac gttaacttcg ccgttgaacc ggggcaaaaa
    12121 ctgcatgttc tgctgcgccc ggaagactta cgtgttgaag agattaacga cgacaaccac
    12181 gctgaagggc tgattggtta cgttcgtgag cgtaactaca aaggcatgac gctggagtcg
    12241 gttgttgaac tggaaaatgg caagatggtg atggtcagcg aattcttcaa tgaagacgac
    12301 cctgactttg accactctct cgaccaaaaa atggccatta attgggtaga aagctgggag
    12361 gtcgtactgg ctgatgaaga acacaagtaa gttccagaat gtagtgattg tcactattgt
    12421 cggttggctt gtgttgtttg tctttctgcc caacctgatg atcattggca ccagcttttt
    12481 gacccgcgac gacgccagtt tcgtcaaaat ggtctttacg ctggataact acacgcgtct
    12541 gctcgatccg ctctattttg aagtgctatt gcactcgctg aatatggcgc tgatcgccac
    12601 cctcgcctgc ctggtgctgg gctacccgtt tgcctggttt ctggcgaagc tgccacacaa
    12661 ggtgcgtccg ctgctgctgt ttctgctgat tgttcccttc tggaccaact cactgattcg
    12721 catctacggg ctgaaaattt tcctcagcac caaaggctat ctcaacgagt ttttgctctg
    12781 gctgggcgtt atcgacacac caattcgtat catgttcacg cccagtgcgg tgattatcgg
    12841 tctggtttac attctgctac cgtttatggt gatgccgctg tactccagta tcgaaaaact
    12901 ggataagccg ttgcttgagg cggcgcgcga tctcggtgcc agcaagttac agacttttat
    12961 ccgtatcatt attccactga cgatgccggg aattattgcc ggatgtctgc tggtgatgct
    13021 gccagcgatg ggcctgttct atgtatccga cctgatgggc ggtgcgaaaa acctgctgat
    13081 cggtaacgtc atcaaggtcc agttcctcaa tattcgtgac tggccgtttg gtgcagccac
    13141 cagcattacg ctgactatcg taatgggcct gatgttgctg gtttactggc gcgcttctcg
    13201 tctgctgaat aaaaaggtgg aactcgaatg atcggtcgac tgcttcgcgg cggttttatg
    13261 accgctatct acgcgtacct gtatatccca atcattattt tgattgtgaa ctcctttaac
    13321 agctcgcgct ttggcatcaa ctggcagggt tttaccacca aatggtatag cctgctgatg
    13381 aacaacgaca gcctgttaca ggcagcgcag cattcactaa caatggcggt gttttcggcg
    13441 acatttgcta cgcttatcgg ttcactgacg gcagttgcgc tgtaccgtta tcgctttcgt
    13501 ggtaagccgt tcgttagcgg aatgctgttt gtggtgatga tgtcaccaga tatcgtgatg
    13561 gcgatttctc tgctggtgct gtttatgctg ctgggtattc agcttggctt ctggtcgctg
    13621 ctgttctcgc atatcacctt ctgcctgccg tttgtggtgg tgacggtgta ttcgcgcctg
    13681 aaaggttttg acgtgcggat gctggaagcg gcgaaagatc tcggtgccag tgaatttacc
    13741 attctgcgaa aaatcattct gccactggca atgccagcgg tggcggcggg ctgggtgtta
    13801 agctttaccc tgtcgatgga cgatgtggta gtttcttcgt tcgtcaccgg accaagttat
    13861 gaaattctgc cgttaaaaat ttattcgatg gtcaaagtcg gcgtatcgcc ggaagttaac
    13921 gcgctggcaa ccatattact ggtgctgtcg ctggtgatgg ttattgccag ccagcttatt
    13981 gctcgtgata aaacgaaagg taacacaggg gacgttaaat gaaaaaatgg tcacgccacc
    14041 tgctcgcggc gggtgctctg gcactgggca tgagcgccgc tcacgccgat gacaacaaca
    14101 cgctgtattt ctacaactgg accgagtacg tgccgccagg actgcttgaa cagttcacca
    14161 aagaaaccgg tattaaggct atctattcga cttacgagtc gaacgaaacc atgtacgcca
    14221 agctgaaaac gtacaaagac ggtgcctatg acctggtggt tccttcaacc tattacgtcg
    14281 ataaaatgcg taaagaaggg atgatccaga agatcgacaa gtcgaagtta acgaacttca
    14341 gcaatctcga tccagacatg ctcaacaagc cgtttgaccc gaataacgac tactccattc
    14401 cgtatatctg gggtgcgacg gcgattggcg ttaacggtga tgcggtggat cctaaatctg
    14461 tcaccagttg ggcagatctg tggaagccag agtacaaagg tagcctgctg ctgaccgacg
    14521 atgcccgtga agtgttccag atggcgctgc gcaaactggg ctactccggt aacaccaccg
    14581 atccgaaaga gattgaagct gcatataacg agctgaaaaa actgatgcca aacgtcgcag
    14641 cgtttaactc cgataacccg gctaacccgt acatggaagg cgaagttaac ctcggcatga
    14701 tctggaacgg ctctgctttc gttgcacgcc aggcgggtac gccaattgac gtggtgtggc
    14761 cgaaagaagg cggcattttc tggatggaca gcctggcgat cccggcaaat gccaaaaaca
    14821 aagaaggcgc gctgaaattg atcaacttcc tgctgcgccc ggatgtggca aaacaggttg
    14881 ccgaaactat cggttatcca acgccaaacc ttgcggcgcg taagctgtta agcccggaag
    14941 tggcgaacga taaaacgctc tacccggatg ctgaaaccat taaaaatggt gaatggcaga
    15001 atgacgttgg cgcagccagc agcatttatg aagagtatta tcagaagctg aaagcaggac
    15061 gttaattcag caaacgggcg gtggtattac cgcccgtttt ttagttagca aggatgtggc
    15121 taatgagtca ggacagtaaa gttttatccc gctctttcgt cggtattgtg gtccccttgt
    15181 aggcctgata agcgtagcgc atcaggcaat gcttcctgct ttataatccc ttcagcaact
    15241 tttcaacaaa ctcaggcacc acctggcttg ccgggccgta atatttctcg gcaaattcat
    15301 taccgacctg acttggttcg agattcagtt ccacggtgtg cgcgccatgc agtttcgctt
    15361 cgtgaacaaa cccagccgcc ggataaacat gcccggaagt accaatggca atgaaaatat
    15421 cagccatcga caacgccata taaatttcat ccatgccgag tggcatttcg ccaaaccaaa
    15481 cgacgtgcgg gcgcaagggt gccgggaact ggcaacaatg gcatttatct tctggcgtaa
    15541 cgtctccggt ccagtcgaga acctgaccac tttgcgaaca acgaactttc agcaattccc
    15601 catgcatatg aatgacattg gtattacctg cgcgttcatg caggttgtct atattctgcg
    15661 ttaccagcaa aaagcgatcg ccgagggcgt cttgcagttt agccagcgca agatgcgcgg
    15721 cgttaggctg aatttctggc tgctgcagct gtcgacgacg ggcgttataa aacgcttgca
    15781 ccagttcagg atcgcgatcg aaaccttccg gcgttgccac atcttcaacc cgatgttctt
    15841 cccacaggcc atctgcggcg cgaaaggtac gaatacctga ttccgcagaa attcctgccc
    15901 ctgtcagtac gagtactctt ggtttttcca ttgcttccgg caccacttta tctctgaaaa
    15961 aaatacgctg acgcaaacgc tcgcgcaggc ggcgtttatt tttacgaaaa cgacttaacc
    16021 gatgaccccg acgcgacagc atagcaacct ctgttgttta atcggttaga tgtaggaagg
    16081 ccgcaccacg cattcctccc gcatcaccgt ggcgcgcgcg ttcaatgcgc ggaacacgag
    16141 ctacaggtaa gagatgacga ggcagcctgt ccgccagttg cgttgtgatt gccgggaaat
    16201 tcgataagcc accaccaatg acgaccaggt cagagtcaac gatggtcagg atatttccca
    16261 gacaaaccgc taataaatcc agataacgct caacgtgcgc ccttgcctgc tcatcgcctt
    16321 gatcataaag cgcaataatt tcgggagcct gcaacggttg atgataatag tgttgccaga
    16381 gccacgcaaa gccccgacct gatagataat tttcgataca accaatttga ccacagccgc
    16441 agcggcgtaa cgggaaatcc agccccatca tggttaacgc atcaaccggc agacgcatat
    16501 ggccaaactc gccggtaatg tagcttttac cggtaatcgg tttaccgttg aaaatcagcc
    16561 cgccgccaac gccggtgccg agaattaacc ccatcaccag tggatattgc gtaaattcat
    16621 catcccaggc ttctgaaagg gcaaaacagt tggcatcgtt atcaaggcgt acatcgcgat
    16681 caagacgtgc gctcaggtcg gcacgcagcg gtttaccgct ggcagcgggg acattggcgg
    16741 catacagcgt accatcttct gtttccggca ttcccggaat accgatgcca acagaacctt
    16801 tacagccaaa acgttggtca gcttcggcta ccagctcgca tactgcatct aaaaatgcgt
    16861 catagctgtc acgcggtgtc ggcacccgct tttcccactg caactgccga ccgctatcaa
    16921 acacgccaag cgcaattttt gttccaccaa tatcaaaccc gtaatacatt gccgctcctt
    16981 gttttaatgt actgccttta ctggccgcta aggactcgcg cagggtcaat attactggcg
    17041 cgccgcgccg gataccaact tgccaaaaga ctcagcaaca atgctgtgac cagtacgtag
    17101 aagacgtcca gccagtgcaa ttccgatggc aggaagtcaa taaaatagat atcgctggag
    17161 aggaactgat gaccgatcag cttttcaatc cgctcaataa tcggggtaag ttgcagtgaa
    17221 acaactacgc cgataatcac accacacagg ctaccgaaca gccctgccag caatccatac
    17281 cagacaaaga tggcgcgaat taaaccatct ttcgccccca gcgttcttaa tactgcgata
    17341 tcgccactct tgtctttcac cgccatcact aaggtggaga cgatgttgaa acaggccacg
    17401 ccaatcacca gtaccatcgc cagatacata atggcgcgga tcatctggat atcgcgatac
    17461 atatagccgt aagtaccaat ccaactttta atataaacat agctgttggt cacttcaccc
    17521 gcatcgcgta ccagcttatt ggcgttgaaa acatccgtca ttttaagggc aatacctgac
    17581 acgctggaac ccatatcaag atattgttgg gcatccgcca gcgggatcat ggcaaaactg
    17641 tgatcgagtt gaccactcaa ctgcaaaata ccggcaatgt gcaaacgcac acgttttggc
    17701 tgcatcagtt tatgctcggg attcgagttg gggatcatga tcgacaccca atcgccctgc
    17761 ttcactttca gcgcatccgc cacgcctttg ccgatgataa tttgctgttc gcccgcttta
    17821 aaattgcgcc aggcatcccc ctgaacaaac gagggtaatg cgctcagacg ctgttcctgt
    17881 tgcgggttaa cgcccttcac ctgaattgcg cgcagattgg taccactttc taccagtccg
    17941 gtgaaattga tatacggcgc agcggcggca atacccggca ctttttgcac gttatccagt
    18001 gcttcctgcc agttagtcca tggctgattt accgcctcaa tttcaccgtg cggtaccaca
    18061 gcaagaatgc ggttattcag ttcgcgttca aagccgttca tcgcgcttaa gccgacgatc
    18121 aataccgcca cgccgagggc aatgccaatg gtagaaatga cagagattag cgacaccatg
    18181 ccgccgcgtc gccgtccgcg actaaaacgc aggccaatca ataacgataa aggcatcgcc
    18241 attactccgc ccccatcagg ctcagttccg ccgtcagacg tccatcacgc atttccagtt
    18301 ggcggctcat acgtttcgcc agttgcaggt cgtgagtaac caccaggaag gcggtgccct
    18361 gcaagcgatt caattcccca agcaactgga agatgctgtc ggcgttacgc gcatcgaggt
    18421 taccggtagg ctcatccgca agtaccagac gcggattatt gaccagcgca cgggcaatcg
    18481 ccacacgctg gcgttcgccg ccagaaagtt cagatgggcg gtgattcgca cgatgctcca
    18541 gccccaccgc ttttaacatc tccagcgcac ggctgttgat ttcagcgggc tttttcttgc
    18601 caatcagcag tggcatagcc acgttttcca gggcagtaaa atccggcagc aggtggtgaa
    18661 actgataaat aaagcccagc ttctggttgc gcagttctgc tttcgccgcc gaagacagtt
    18721 tgctcatcgg ttgaccgtta aagatcacat cgccggaggt tggtgtatcc agcccgccca
    18781 gcaggtgcag caaggtactt ttaccggaac cagagctacc gacgatcgcc atcatttcac
    18841 cttcgccgac gctgaaactg acattgtgca gtacatcggt ttgcacactg ccttcctgat
    18901 agcgtttgca caggttgtcg cattgcaaca ggatcttatt cataacgtaa agcctcagcg
    18961 ggttgagtgg cggcagcgcg ccatgaaggg taaagcgtag acagcagcgc gatagccatc
    19021 gccaccagcg caataacaat gacctgtaaa ggttcgatag cgaccggcag cgccgcgcca
    19081 tcaagcagga cgccgattat cggcatcaga ttatttaact ggctggcaag cagtgcgcca
    19141 agcgccgctc cgaggatcgc accgataatc ccggcgctgg ccccttgcac cataaagacc
    19201 atcatgattt gtcgcggagt taagccttgc gtttgcagga tcgcgacttc gccctgcttc
    19261 tccattacca tcagccctag tgaggtaata atgttaaacg ccgcaacggc gacaatcagg
    19321 ctcagcagca agcccatcat atttttttcc atgcgtacgg cctggaacag ctcgccttta
    19381 cggtcacgcc agtcctgcca tttgctgcct tcaggcagtt tttgctgact taaagagtca
    19441 actttcagcg gctcatccag ccacaaacgc cagccggtaa tattgcctgc cggataacgc
    19501 atcagacgcg aggcatcctc aatattcacc agcatttcat agccatcgac ttcactgttg
    19561 gcggcgaaag tgccaatcac attgaacagg cgctggcttg gaatacgccc catcggcgtg
    19621 aactggctgg cagatggtac catcacgcgg atttgatcac cgcgattaac gcctagctgt
    19681 gaggcaagtt gttcgccgag gatgacatta tatttccccg gctcgaggtc agtttgtttc
    19741 acattgacca gatacggcgt aagtggatct ttttgcgccg ggtcgatacc gagcatcacc
    19801 ccgaccgcca cgctgcgcgc gctttgcagt accacatcac cggtagtaat aggtgcgacg
    19861 cgattaacgc cgtccagttt gactgccgtt tccgggagtt gctgcgggtt aagagagcca
    19921 tgctcagaag agagaattgc ctgtggcatc aggccaagga tgttgttttg cagctcgcgc
    19981 tcaaagccgt tcatcactga caatactgtg accagcgcca tcaccccgag ggtaatgccg
    20041 atggtagaaa gccaggagac gaaacgaccg aagcgatccg ctgcacgccc acgcatgtaa
    20101 cgcaggccaa taaatagagc gacaggttgg tacatgaaat ccgtctggtt gctgtagcaa
    20161 agacacgagt atataagcga aagcaagatg ggtaaacgtc tgaactgtaa gtgatcgtca
    20221 gggctaatct gaaaagcccc cgtaaatcaa tcggctaaat tgccatgttt tatcatcaat
    20281 cttgccgcct gatcttaatc tgaaaaatca tccaatacag acagataccc attacacctc
    20341 gggtcataac ccgcaggatc aaccgcatgt aaattaaaaa agagcgtgtt gtcctgatga
    20401 aacaaaaaga gctatggatt aaccagatca aagggttatg tatttgtctg gtggtgattt
    20461 atcactcggt cattaccttt tatccgcata tgaccacttt tcagcatccg ttatcggaag
    20521 tcctgagcaa atgctggatc tatttcaatc tttaccttgc accctttcgt atgccggttt
    20581 ttttctttat atccggctat ttgattcgcc gctatatcga cagcgtaccg tggggaaatt
    20641 gtctcgataa acggatctgg aacatcttct gggtgctggc gctttggggc gtggtgcagt
    20701 ggctggcgtt aagtgcgctg aaccagtggc tggcacctga gcgcgattta agcaatgcct
    20761 ccaatgccgc ttatgccgat tccaccggtg agttcctgca cgggatgatc accgccagca
    20821 ccagcttgtg gtatctgtat gcgttaattg tctatttcgt ggtatgtaaa atttttagcc
    20881 gtctggcgct gccactattc gccttgtttg tactgctgag tgtggcggtt aatttcgttc
    20941 ccacgccgtg gtggggaatg aacagtgtga tccgcaattt gccttattac agccttggcg
    21001 catggtttgg cgcaacaata atgacctgtg ttaaagaggt gccgttgcgc cgccatctgc
    21061 tgatggcttc tttgcttacc gttctggcgg tcggtgcctg gttgtttact atctcgctgc
    21121 tgttgtcgct ggtatcgatt gtggtgatca tgaagctgtt ttatcagtac gagcaacgat
    21181 tcggtatgcg ctccaccagc ctgctgaatg tgattggctc caacaccatt gctatctaca
    21241 ccacccatcg cattctggtt gaaatattca gcttaactct gcttgcgcaa atgaacgcag
    21301 cacgctggtc gccgcaagtc gaactgacac tcctgctggt ttatcccttt gtcagtttgt
    21361 tcatctgtac tgttgcgggc ttgctggtaa gaaaactttc acagcgtgca ttcagcgatc
    21421 tgttgttctc cccgccttca ctgcccgctg ccgtcagtta ctctcgttaa accatcgccc
    21481 cagccggggc gattcaattt gctaaaccat gtcgattacg gataatgaac agcattgctt
    21541 atcagagata tgcccccata tgttgaggca tatcctaacg agaatctgac aaccgttatg
    21601 cctgaacaat atcgttatac actgcccgtc aaagcgggtg agcagcgtct gctgggcgag
    21661 ttaaccggcg cagcctgtgc aacgctggta gcggaaattg ccgaacgtca cgccggtccg
    21721 gtggtactca ttgcaccaga tatgcaaaat gctctgcgtt tgcatgatga aatcagccag
    21781 ttcaccgatc agatggtgat gaatctggcg gactgggaaa ctcttcccta cgacagtttt
    21841 tcgcctcatc aggacattat ctcctcgcgc ctttccaccc tttaccagct accgacgatg
    21901 cagcgtggcg tactgattgt tccggtgaat acgcttatgc agcgcgtttg cccacacagt
    21961 tttctccacg gtcatgcgct ggtgatgaaa aaaggtcagc gcctgtcacg agatgcatta
    22021 cgaacccaac tggacagcgc cggttatcgc catgttgacc aggtgatgga gcacggcgaa
    22081 tacgccacgc gcggcgcgtt gctggatctc ttcccgatgg ggagtgagct gccttatcgt
    22141 cttgatttct ttgatgatga aatcgacagc ctgcgggtgt ttgacgtcga cagccagcgc
    22201 acgctggagg aagtagaagc gatcaatctg ctgcccgcgc acgaatttcc gaccgataaa
    22261 gcggcaattg aactgttccg cagccagtgg cgcgatacct tcgaagtgaa gcgcgatccg
    22321 gaacatattt accagcaagt gagtaaaggc acattacctg ccgggatcga gtactggcag
    22381 ccattgttct tcagcgaacc actgccgccg ctgttcagtt atttccctgc caataccttg
    22441 ctcgtgaata ctggcgatct ggaaaccagt gccgaacgtt tccaggctga cacgctggcg
    22501 cgttttgaga atcgcggcgt cgatccgatg cgcccgctgt tgccaccaca atcgctctgg
    22561 ctgcgggtgg acgagctctt ctcagagctg aaaaactggc cccgggtgca gctaaaaact
    22621 gaacatttac cgacaaaagc cgcgaatgcc aatttaggtt tccagaaact gccagacctg
    22681 gccgttcagg cacaacaaaa agcgccgctg gatgcgctgc gtaagttcct cgagactttc
    22741 gacggtccgg tggtgttctc ggtagaaagt gaaggtcgcc gtgaagcgct gggtgaactg
    22801 ctcgcacgaa ttaaaattgc tccgcaacgc attatgcgtc ttgatgaagc cagcgaccgt
    22861 gggcgttatc tgatgattgg cgctgccgaa catggttttg tcgataaggt gcgtaatctg
    22921 gcgctgatct gcgaaagcga tctgctcggt gaacgtgttg cccgccgtcg tcaggattct
    22981 cgccgcacca tcaaccccga tacactgatc cgtaaccttg cggagctgca tattggtcag
    23041 ccggtggtcc atctggagca cggtgtcggg cgctatgccg gaatgaccac gctcgaagcg
    23101 ggcggcatta ctggcgagta tttgatgctc acctatgcca acgacgccaa actgtatgtt
    23161 ccggtgtcgt cactgcatct gattagccgt tacgcaggtg gcgcggaaga aaacgccccg
    23221 ctgcataaac ttggcggcga tgcgtggtca cgcgcgcggc agaaagcggc ggaaaaagtg
    23281 cgtgatgtgg cggcggaatt gctggatatc tacgcgcaac gcgccgccaa agagggcttc
    23341 gcgtttaaac acgatcgtga gcagtatcag ttgttctgcg acagcttccc gtttgaaacc
    23401 acgccggatc aggcgcaggc cattaatgcg gtacttagcg acatgtgtca gccgctggca
    23461 atggatcgtc tggtgtgcgg cgatgttggc tttggtaaaa cagaagtggc gatgcgcgcc
    23521 gctttcctgg cagtagataa ccacaagcag gtggcggtgt tggtgcctac cacccttctc
    23581 gcgcagcagc attacgacaa cttccgcgac cgtttcgcca actggccggt acgtatcgaa
    23641 atgatctccc gtttccgcag cgccaaagag cagacgcaaa tccttgcgga agtggcggaa
    23701 gggaaaatcg atattctgat cggtacgcac aaactgctgc aaagtgacgt caagtttaaa
    23761 gatttaggcc tgctgattgt cgatgaagaa caccgcttcg gggtgcgtca taaagagcgc
    23821 attaaagcga tgcgcgcgaa cgtggatatt ctgacgctta ctgcaacgcc gatcccacgc
    23881 acgctgaata tggcaatgag cggaatgcgt gacctgtcga ttatcgccac gccgcccgcc
    23941 cgtcgtctgg cagttaaaac ctttgtccgt gagtatgaca gcctggtggt ccgggaggcg
    24001 atcctgcgtg aaattttgcg cggggggcag gtttattatc tctacaatga tgtggaaaac
    24061 atccagaaag ctgccgaacg gctggcagaa ctggtgccag aagcgcggat cgccatcggt
    24121 cacgggcaga tgcgcgagcg cgaactggaa cgggtgatga atgatttcca tcatcaacgt
    24181 ttcaacgtgc tggtttgtac aaccattatc gaaaccggga tcgacatccc gacagccaac
    24241 accattatca ttgaacgcgc ggatcacttc ggtctggcgc agctgcacca gttacgcggt
    24301 cgcgtcggac gttcgcatca tcaggcatat gcatggttgc tgacaccgca tccaaaagcg
    24361 atgactaccg atgcacaaaa acgtcttgaa gcgattgcct cgctggaaga tctcggggca
    24421 ggttttgcgc tggcaacgca cgatctggag attcgcggcg cgggtgaact gcttggcgaa
    24481 gaacaaagcg gctcaatgga aaccatcggt ttctcgctgt atatggagtt gctggaaaac
    24541 gccgtcgatg cactgaaagc cggacgcgag ccgtcgctgg aagatctcac cagccagcaa
    24601 acagaagtcg agctgcggat gccgtcgcta ttgccagatg atttcattcc tgacgtgaat
    24661 acgcgtttgt cgttctataa acgtattgcc agcgccaaaa cggaaaacga actggaagag
    24721 atcaaagtcg agcttatcga tcgcttcggc ctgctgccag atccggcgcg taccctgctg
    24781 gatattgccc gtctgcgcca gcaagcgcag aaactgggga tcaggaagct ggaaggtaat
    24841 gagaaaggcg gggtgatcga atttgccgag aagaatcacg ttaatccggc ctggttgatt
    24901 ggtttgctgc aaaaacagcc gcagcattac cgccttgatg gtccgacgcg cctgaaattt
    24961 attcaggatt tgagtgagcg gaaaacgcgt atcgaatggg tacgccagtt tatgcgtgaa
    25021 ctggaagaga acgcgatcgc ttaatggtaa atcgggggcg tttctgcgcc cccatctgca
    25081 acatttacaa attctttgca cttccctgca ctatccgaca ctgtcaccat ccataattca
    25141 ggcttatctg tttattacaa taaccttata tttattattg atttttggtg atgatcaaaa
    25201 cgcgtttttc tcgctggcta acgtttttta cgttcgccgc tgccgtggcg ctaccggcaa
    25261 aagccaacac ctagccgctg ccgccagcga gcagtcgtct ggttggcgaa aacaaatttc
    25321 atgtggtgga aaatgacggt ggttctctgg aagccatcgc caaaaaatat aacgtcggct
    25381 ttctcgctct gttacaggct aaccccggcg ttgatcctta cgtaccgcgc gcgggcagcg
    25441 tgttaacgat cccgttgcaa accctacttc cagacgcgcc gcgcgaaggc attgtgatca
    25501 acattgcgga gctgcgtctc tattactacc cgccgggtaa aaattcggta accgtgtatc
    25561 caataggtat tggtcagtta ggtggtgaca cgctgacacc gacaatggtg accaccgttt
    25621 cagacaaacg tgcaaaccca acctggacgc caacggcaaa catccgcgcc cgttataaag
    25681 cacagggaat tgagttgcct acggtagtgc cggctggacc ggataaccca atgggccatc
    25741 atgcgattcg tctggcggcc tatggcggcg tttatttgct tcacggtacg aacgccgatt
    25801 tcggcattgg catgcgggta agttctggct gtattcgtct gcgggatgac gatatcaaaa
    25861 cactctttag ccaggtcacc ccaggcacca aagtgaatat catcaacact ccgataaaag
    25921 tctctgccga accaaacggt gcgcgtctgg ttgaagtaca tcagccgctg tcagagaaga
    25981 ttgatgacga tccgcagctg ctgccaatta cgctgaatag cgcaatgcaa tcatttaaag
    26041 atgcagcaca aactgacgct gaagtgatgc aacatgtgat ggatgtccgt tccgggatgc
    26101 cggtggatgt ccgccgtcat caagtgagcc cacaaactct gtaaatcctg gataaaaaaa
    26161 atccccgcga cacggcatgt cgcggggaaa aaatcagcag tagcaggcat taatgagggt
    26221 taatgcttat ttataaatta ctgctgttcc atggagggta ttcggaccgg ttacagaagt
    26281 aatacggaaa gattttgcgc ccatctcatc cgctttttgc gccagctgct cttccagcga
    26341 tcccagattt gtccccgcgt tagcactgat agtaccgact ttttgttggc cttctggcgt
    26401 tgactgaact tcgacagccg caaagttggc aaatgacatg gagcttaaaa tcgccgcagc
    26461 gatgagggtt tttacgtttt tcataatagt ggccttatgc agatgaatga cgtcgcaaga
    26521 aattacttaa cgatcgataa ctaaatgata gatgtgatct ggatcacata caagatattt
    26581 tttataacaa tcattaatta atttaaaaat ctttaaattt cagttaaata gaaagcaatt
    26641 atttttatgg gatctggact ggtgaacgga ggtgattatt tttataataa cctttcattc
    26701 acagtcacca ggttcaacgg catcatggca actgactcaa cacaatgtgt aaaaaaaagc
    26761 cgtggccgcc caaaagtgtt cgacagggat gccgcgcttg ataaggccat gaaattgttc
    26821 tggcaacacg gttatgaagc gacttctctt gcggacctcg tcgaagcgac cggagccaaa
    26881 gcgcccacgc tatacgcgga atttaccaac aaagaggggt tatttcgtgc cgttctcgac
    26941 cgctatatcg atcgttttgc cgctaagcat gaagcacagc tgttttgtga agagaaaagc
    27001 gtggagtctg cgctggctga ctattttgct gccatcgcca actgctttac cagcaaagac
    27061 accccggcag gctgcttcat gatcaacaac tgcaccaccc tctccccaga ttcaggagat
    27121 atcgccaata cgttgaaatc acgccatgcg atgcaagagc gcactttgca gcagttttta
    27181 tgtcaacgac aagcgcgcgg ggaaatcccg acccactgtg acgtgacaca tctggcagaa
    27241 ttccttaatt gtattattca ggggatgtcg atcagcgcac gcgaaggtgc atcgctggaa
    27301 aaactgatgc agattgccgg aacgactttg cgtttatggc ccgaactggt gaaataagtc
    27361 gcaaaaaaag cccctcagcc tgagcgaatg aggggccaaa ttgcagagta cagccttgtt
    27421 atactttgtt attcaaaatc agttgcccgt tactatctag cgggatctgc gtacccggat
    27481 cgcggtccat gcggattttg ccctgctgat cgccaatctt ataggtcaca tcataaccga
    27541 gcattttttc tgacttgtca tacaccgttt tacaacgctg ttgcgtagtc gtgtaagtat
    27601 cgctttcctg gagagagccc tggatctggt taccggcata tccaccaccc agcgccccca
    27661 caacagtggc gacatcttta ccacgaccac caccaaactg atgccctatc acgccgccag
    27721 caacagcgcc gagcaccgac ccggtaatgc gattttcatc ctgcaccggt cgacgatggg
    27781 tcactgtgac gttgcgacac tcctgacgcg gtgttttaac cgtttccttg attggggttg
    27841 cagaaacaac ctgagcgtat tgcgggcccc gttcaaacac gttcagactg gccactgccg
    27901 ctacgcccag cgcagctgcg acaccaatcc cgatacccgc caacattgat ttattcacgg
    27961 gaacacctcc ttctttgcag ttatgccaat catgcaagcg aaggagaaaa gcagctatca
    28021 gacagaagat gaaaagcgcg ggaatggctg gaaatactta aggattcgga gaagtctgaa
    28081 ggaatgcctg atgcgacgct taacgcgtct tatcaggcct accgctcgta ccatttgtcc
    28141 agggcggatg aggcgtttat gccacatccg ccagtgtacg tcgattaatg caacttcaaa
    28201 cgcggacgga taacgcggtt aatactcccc accagcatca ttaatccggt tttaaagtaa
    28261 ccatgcagcg caatctgatg cattcggtat agcgagatat atacaaagcg cgcaattcgt
    28321 ccttcaatca tcattgagcc gcgcgtcagg ttacccatca ggctaccgac agtggagaag
    28381 ttcgacagcg ataccagcga accatgatct ttatactgat aatttttcag cggtttaccg
    28441 ttcatctgcg ccagaatatt gttcattgcg caggtcgcca tctggtgtgc agcctgagca
    28501 cgcggcggaa caaagccccc ttccggacgc gggcatgacg cgcagtcgcc aatagcgtaa
    28561 atgtctggat cgcgggtggt ttgcagcgtc ggttccacca ccagctggtt gatacggttg
    28621 gtttcaagac caccgatatc tttcaggaag tctggcgctt tgatcccggc tgcccacacc
    28681 atcagatcag cctcaatata ttcaccatct ttagtgtgca ggccgccttc atcagcactg
    28741 gtgaccatgg tttgcgtcag cacgcgaacg ccaagtttcg ttagctcgtt gtgggccgca
    28801 gcagagatac gtggcggtaa tgcaggcaaa atacgttctc ccgcttctac cagcgttacg
    28861 ttcagggctt cgttggtcag gcctttgtaa ccgtagctgt gcagttgctt gaccgcattg
    28921 tgcaattcag cggagagttc tacacccgtc gcgccgccgc cgacaatcgc aatgttcact
    28981 ttaccattcg cgcccaggtt ggcggagtat ttcaggaaca gattcagcat ctcctggtgg
    29041 aagcgacgcg cctggtgcgg gttatcgagg aaaatgcagt tctctttgac acctggcgta
    29101 ttgaaatcgt tagaggtgct acccagcgcc attaccaggg tgtcataggc gattttacgt
    29161 tccggaacca gcagttcacc tttctcatcg cgcagttctg caatagtgat tgttttcgct
    29221 tcacgatcaa tatcaatgac ggaacccagc tggaactgga aaccatgatt gcgcgcatgg
    29281 gccagatagc tcaacgcatc gacgccttca tcaagcgagc cagtcgccac ttcgtgcagc
    29341 agcggtttcc acagatggct gtggttacga tcgaccagcg taattttggc ttttttcttg
    29401 cgtcccagct tatgccccag ctgtgttgcc atttccagcc caccagcacc gccgccgaca
    29461 atcacaattt ttttcaatgg cgtagtcaac gtgaccccct taaatttatt aaccaattgt
    29521 taattaatgg ttataaacat agctcttaat taacaacagg ttacagcaat gaaaatgttc
    29581 ttcgctattg agaataacat gaatggtgca ttggtcatac caaaattgat gttaatcaag
    29641 ttttgttgct gaaaagatag gcgactttac cgggtgagat caaaaaacag agtttattgt
    29701 ttattatttg agcaagtccg gaagggtgat atagggtgac gcgtatatcg agggcgtttg
    29761 gtcgtcctga taagatgcgc agcatcgcat caggcattga tgctcgactg tcggatgcgg
    29821 cgtaaacgcc ttatccgact tacagaattc ggagcgcatc aggtaccggc aaggttacct
    29881 gatgcgacga gggcatttac ccgagggttt tgaacgcctt aatgcgctgc aaatgcgggg
    29941 agatattctt gaatttgtgc gtctgctctt cgtcccagac aatctcgtaa taatgatgca
    30001 actcttcaga tgtccgctgg ctgttaagcg cttcatcatt acgcgacaaa atcaccaggc
    30061 aacgatcgcg attcttctcg cggaagttgg tcacacactt agtggcaata tcggcatact
    30121 cttccgggcg atcaatcttg ccttccatgt tctcataagg gaacaaatta gggttgaaga
    30181 tcacctggcg gatatcgcaa agaaaaccaa tccgttccgc ccagtatccg cccagaccaa
    30241 cgccgcaaat cagcggacgc tcgtcgacgt tcagttgcaa cattttgtcc acttctttga
    30301 gcagatgctg catatcatgt ttcggatgcc gcgtactgta gcttatcaag cgtacatccg
    30361 ggtcaataaa ctgcaattgt aagactttct cgtggttacc cggactgtta gagtcaaaac
    30421 cgtgtaaata gataatcatc gcttcctcac ataagccagg gttagtgacc tgctttctct
    30481 tcctgccagc gttcatgtaa ctgattcaga cgggtgctga tcgccttcca gcgagccgag
    30541 tccatcagtt cctgtcgcga aaatgaacct ttatgataca aacgtgtaac acgttctgcc
    30601 ttgatcggtg acagattatc taacacgctg acggcccctt tacgattatt gcagaccagg
    30661 atcatatcgc aacctgcgtc cagtgatgcc tgcccgcgtt cggcataact gcccataatc
    30721 gcggcacctt ccatcgataa atcgtcagag aaaattacac cgtcaaaaca cagttcctga
    30781 cgcaaaacgg ttttcagcca gtaggaagaa ccgctcgccg gacgcggatc aacatcactg
    30841 tagatcacat gcgcaggcat aatggcgtcg agtttatttt cgcggattaa ggaactgaag
    30901 accgacatat ctttagcgcg aatctccgct tgtggacgtg gatcgcacgg tgtttctttg
    30961 tgtgagtctg ccgttactgc accgtgtcct gggaagtgtt tcccggtcgt tttcattccg
    31021 gcttcatgca taccatcaat aaaccggctg gcaattgcca gggctttttg tggatcggca
    31081 tgataagaac gctcgccaat cgccgcgctg atatgcccga catccagcac tggcgcaaag
    31141 ctgatatcaa tatccatagc gatcatttcg ctggccatca gccaacccgc ctcttgcgcc
    31201 agtttgccgc cctcttccat tcccaacagc gcagcaaagg attgtgctgc cggtaagcgg
    31261 gtaaaacctt cgcgaaaacg ctgcacgcgt ccaccttcct gatctaccgc caccaccaga
    31321 tgattgcgcg atgctgcgcg gatctggcgc accagttcac gtaactgggc aggatcatga
    31381 tagttacgcg taaagagaat cagccctccc accagcggat gcgccagtat ttcacgctct
    31441 tccgcgtcca gttcgtaacc ttcgacatcc aacattactg gacccacact gctctcctta
    31501 ttgttttatt aatagctgcc gccaggtgtc atcggccagc ctgataaatt gttgatcgcc
    31561 gatttgtcgc cagcggtact caaaccaccc tgctttgagc atcagcagcc agggaaacca
    31621 tcgcctgacc tgacgccata attgcgccgg ataaatcttc gcgcgagtgg catagtcatt
    31681 gaccaattgc cggtgctgtt cagtattttc cacccacacc gccgccagtt ccagcgcgat
    31741 atcaccatct ccggcatact cccagtcgat gagttttaac cctgacgcgc tatgcactaa
    31801 atttccggcg tggacatcca tatgcaatgg acttaagcgt aaaggccgtg gttcccgcgc
    31861 tttgcgcaga cgttttaaca ttcgcagcca acccacagtc cgccgcgccg gatcgctttg
    31921 ctgccagtac agttccagta agggcaacag cgttattcgc cagccaaaac gtggttgttg
    31981 atgaagataa tacagcaagc ctgccagttc gttggtatcc ggcaaatacg tttttaccgc
    32041 gccgggcaga tagtcgacta ccatccagtc acggagatat aaatgcggct tcggtgcaat
    32101 gcttgcgggt agttgtgata aagcccgata ctggcgcaag aacgcggact gcggcgcatc
    32161 aggatcgtgc ggctgacgca caacaaaacg ctggccctga tgttcaatga gaaaactacc
    32221 gccactaagc ccactattaa acgtcgtgac gggatgaaac tgcgggaaaa agcgcgacag
    32281 caattcgtcg cgcgtgatgg gattattgct gcgaaacggc acctttacct gaccagataa
    32341 tttcgcccgt ctgcaccagc atcagctgca tttgtagggt cggagcgtta acgttgccag
    32401 aggcgctgga gtacagcacg taatgagcgc cgacattgcg ggcaatgcct atggctttac
    32461 tacgggtacc taaactgtcc tgcggcgaca aacctaactg ttgcttagcc atcgacagct
    32521 gctgggcgga aaccagggta aatttcccgt tattagccag cgcatttcgc agcgtttcgg
    32581 tcgcttctgc ggcattcagc gaaccgttag tacggttgtt aacgctatca accagcagga
    32641 cgctacccgc agtcaccccg tcagccccaa gcatcttact gaccatcggc tgcattgcgc
    32701 cattccagtc ataatggcga atatgcggcg caggcggtgc cgtttgatct tcgtgctcaa
    32761 ttgggcctgg ctgctgcggg atcgtcggca ccgagggcac tgtggggaca ggctgttgtg
    32821 gctcggctgg ttgttccggc gctggtttca cttcttctac cggtgcaggt tcacgttgcc
    32881 ccacacaccc ggcgagaaac atcgccagcg cggtaatcaa ggcgtagcga ctcatttttg
    32941 tcatcaagat tcacccctta caaatataga taaagtctga ctttgtgcgc ccccagaaaa
    33001 ttggcgctgc cgtacagcgt taccgccgaa tgtgcgggaa tgctaacgct gcgtggcctt
    33061 tccagaggat gcatctccag ccctctggcg tcataccagt aaaaacgata atgaacggta
    33121 acgggttctt gcctttcgtt ataaagcgtt gaggatgctg aaggttgaat atcagacgtc
    33181 gaaaggacgg gcttttctgc actgatgccc gcagccaata aagatgactc catcaccagc
    33241 gattgctcat cattcaccgg aatttccgga tgtgaacgac agcccaccag cagtaacaac
    33301 gccagagaca ccagcccaaa gcatcctttt ctcatcgtta cagaccttta tgcgccagca
    33361 ttggtcccag cggacggcca cccaacaagt gcatatggat gtggtaaacc tcttgtccgc
    33421 catggcggtt ggtattcatg atcagacgat agccatcttc ggcaatacct tcttgctcag
    33481 caatttttgc cgctacggtg atcatgcgtc ccagcgcctg ctcatgctca gctgacacgt
    33541 cgttcacagt cggaatgagg atattcggaa tgatcagaat atgcgttggc gcttgcggcg
    33601 aaatatcgcg aaacgccgtt accagatcat cctggtagac gatatcggag gggatctcac
    33661 gacgaataat tttgctgaat atagtttctt ctgccacgac attttccttt ttcaaatagc
    33721 catgcgttgc gccatgctac gccgcgccac actgcgagta tagagtatgg tcgagttaaa
    33781 cctgctcttt caaccttaac tcacgatttc ttaagcaaaa aatcgttttg ctgccgtata
    33841 tatcgccatt attcccattt ctgcctgcga atgcgctgaa aagacattct ctgtttggtt
    33901 tacacgacaa atatgaatgc gcatatttct catttgcatt tacaaacaaa attattcgca
    33961 cactaaatat aaccgctcat caccctcctc cggcatgagc ctgacgacaa cataaaccaa
    34021 gagatttcaa atgctttcaa cacaatttaa cagggataat caatatcaag ccatcaccaa
    34081 accgtcacta cttgccggtt gcatagcact ggcactatta ccttctgccg cttttgctgc
    34141 accagccact gaagaaacgg tgattgttga gggttcagcc acagctccag atgatggcga
    34201 aaatgattac agcgtaacgt ctacctctgc gggtaccaaa atgcagatga ctcaacttga
    34261 tattcctcag tcggtcacta ttgttagcca gcagcggatg gaagatcagc agttacaaac
    34321 gctgggcgaa gtgatggaaa acacgctggg gatcagcaaa agtcaggcgg attccgatcg
    34381 tgctctttat tattcccgtg gattccagat cgataactat atggttgatg gtatccccac
    34441 ctattttgaa tcgcgctgga atctgggcga cgcactttct gatatggcac tctttgaacg
    34501 cgtagaagta gtgcgtggcg cgacaggact catgaccggg acgggtaatc catctgcggc
    34561 aattaatatg gttcgaaaac acgcgaccag tcgtgaattt aaaggcgatg tctcggcgga
    34621 atacggtagc tggaacaaag aacggtatgt ggcggattta caaagcccac tcaccgaaga
    34681 cggtaaaatc cgcgcgcgaa ttgtcggcgg ctaccagaat aacgactcat ggctggaccg
    34741 ctacaacagt gaaaagacct tcttctcggg cattgtcgat gctgatttag gcgatcttac
    34801 gacgctttca gccggttacg aatatcagcg cattgatgtt aatagcccaa cctggggcgg
    34861 tttaccgcgc tggaatactg atggcagcag caacagttac gatcgtgcac gcagtaccgc
    34921 acctgactgg gcgtacaacg ataaagagat caacaaggtc tttatgaccc tgaagcagcg
    34981 gtttgctgat acctggcaag cgacactgaa tgccacccac tctgaagtcg aatttgacag
    35041 caaaatgatg tatgtcgatg cctatgtaaa caaagcggat ggtatgctgg ttgggccata
    35101 cagtaattat ggacctggct ttgattatgt cggcggcacc ggttggaaca gtggcaaacg
    35161 taaagttgat gcgctggatt tgttcgctga cggtagttat gaattgtttg gtcgtcagca
    35221 caatctagtg tttggtggca gttacagcaa acaaaacaat cgttacttca gttcatgggc
    35281 caacatcttc ccggatgaaa ttggcagttt ctacaacttt aatggcaatt tcccacaaac
    35341 cgactggtca ccacagagcc tggcacagga cgataccaca catatgaaat cgttatatgc
    35401 tgctactcga gtcacccttg ccgatccgct gcatctgatc ctcggcgcac gttataccaa
    35461 ctggcgggtt gatacgctga cttacagcat ggagaaaaac cacaccacgc cttacgctgg
    35521 tctggtgttt gacatcaatg acaactggtc gacctacgcc agctatacct ctattttcca
    35581 gccgcaaaat gatcgtgaca gttcaggcaa atatctggct ccaatcaccg gtaacaacta
    35641 cgagctgggt ctgaaatcgg actggatgaa tagccgtctg accaccacgt tagccatctt
    35701 ccgtattgag caggataatg tcgctcagtc caccggtaca cctatccccg gcagcaacgg
    35761 cgaaaccgcc tataaagcgg tggatgggac agtcagtaaa ggggtggaat ttgaactcaa
    35821 cggcgcaatt accgacaact ggcagctgac atttggcgca acgcgctata ttgcagagga
    35881 taacgaagga aacgccgtta atcctaatct gccacgcacc acggttaaaa tgttcaccag
    35941 ctatcggttg cctgtcatgc cagagttgac agtcggcggt ggtgttaact ggcaaaatcg
    36001 cgtgtatacc gacaccgtga caccgtatgg caccttccgc gccgagcaag gtagctacgc
    36061 gctggtggat ctcttcaccc gctaccaggt gacgaaaaac ttctcgttac aggggaacgt
    36121 caataacctg ttcgacaaaa cctacgatac caacatggaa ggttctatcg tctacggcgc
    36181 accgcgtaat ttcagcatta ccggcacgta tcaattctga ataacacctg taaaaaaggc
    36241 agccatctgg cggccttagt ctccccaacg tcttacggat tagtggttac ggatgtactc
    36301 atccatctcg gttttcaggt tatcggattt agtaccgaaa atcgcctgaa caccagaacc
    36361 agcaaccact acgcccgctg cgcccagttt cttcaggcca gcctgatcca ctttagacac
    36421 atcagcaacg ctgacgcgca gacgggtaat acatgcgtcg aggttagtaa tgttttcttt
    36481 accaccaaat gcagcaacca gagccggtgc catttcgctg gtacctgttg cttttgcatc
    36541 ttcagtcgcg tcttcacgac ccggcgtttt cagatccagt gctttaatca gcacgcggaa
    36601 gatggtgtag taaacaatcg cataaccgat accgacgatc gggaacagcc acagtttgct
    36661 gctgttacca gacagaacga taaagtcgat cagaccgtgc gagaacgacg taccgtcacg
    36721 catccccaga agaatacaga ttgggaatgc caggcctgcc agaatcgcgt ggatgatgta
    36781 caggatcggc gcaacgaaca tgaaggagaa ctcgatcggc tcggtgatac cggtcaggaa
    36841 cgaggtcagc gccgcggaga tcataatacc gcccactttc gcgcggtttt ctggtttagc
    36901 agagtgccag atagcaattg cggcagctgg cagaccgtac attttgaaca ggaagccacc
    36961 agacagttta cccgcagtcg ggtcacccgc catataacgc ggaatgtcgc cgtggaaaac
    37021 ctgacctgct gcgttggtgt attcaccaat ctgcatctgg aaaggtacgt tccagatgtg
    37081 gtgcagacca aacggcacca ggcaacgttc gatgaaaccg taaatgccaa acgcaactac
    37141 cgggttctgg taagcagccc actgagagaa ggtctggatt gcagaaccaa tcggcggcca
    37201 aatgaaggac agcacaacgc cagtaaagat ggcagccagg ccagaaatga tcggcacaaa
    37261 gcgtttaccg gcaaagaagc caagatactc aggcagctta atacggtaga aacggttaaa
    37321 catgtacgct gcgatcgcac cggagataat ccctccgagt acgccagtat ccgccaggtg
    37381 tttagaggcg atttcttcag caggtaaatg cagtaccagt ggcgcaacca cggccatggt
    37441 tttaaccatg atgccatagg caacaactgc ggccagcgcg gatacgccat cgttattggt
    37501 aaagccgagg gcgacaccga tcgcaaaaat cagtggcatg tttgcaaaga cggaaccgcc
    37561 tgcttctgcc ataacatgcg atacaacggc gggcagccag ctgaaattcg cggaaccgac
    37621 gcccagcaga atacctgcga taggcagtac ggataccggc agcatcagcg atttaccgac
    37681 cttttgcagg ttagcaaatg cattcttaaa cataattgag agtgctcctg agtatgggtg
    37741 ctttttttac gttctcacgc gtggcaaggg gggagagcct cgccgtgtac agggcatcta
    37801 agcgcccttt atttattaca cagagtaaaa taattcagtg ccgatttgtt tgacggctat
    37861 cacgtttcaa cttcagatca ttggagaagt tgcacatttt gacaaaacat atgtcacaat
    37921 atttcaaacg tggttattca ccgcccaatt ttcggcggtg aactttactc gttttagcca
    37981 ttaattacga gctttaaaaa aactcattca acggatggat tgaaggcggg aagcgtcgat
    38041 gtgaaacaga cgggcgaagt tatcggtggt tacctgcgcc agttcttcaa cggcaacacc
    38101 tttcaacaca gccatgtatt ctgcaacgtc acgaaccatc gcaggttgat tctcttttcc
    38161 tcgatgcggt accggcgcaa ggtaaggtga gtcagtttcc accagtaacc gatccagggg
    38221 gacataacgc gcagcatctc gaagttgctc cgcattacgg aaggtcacaa tgccggaaaa
    38281 ggagatgtaa aatccgagat ccagtaattt acccgccgtt tctctgtcct ctgtaaaaca
    38341 gtgtagtacg ccaccgcaat ccgtcacttt ttcttcgcgc agaattgcca gcgtatcggc
    38401 gcgggcgtca cgggtatgga cgataaccgg cttgttcagt tcacgaccaa tctggatatg
    38461 atggatgaag gactcttgct gacgaacttt agtttccggt gtgtaataat aatccagccc
    38521 ggtttcaccc agcgctacaa caccctcttc tgccgccaga cggcgtaaat cttctacatc
    38581 gtagggatca ttctggttga gtggatgcac gccacaagaa aatacgacgt tgtcacgttc
    38641 gcctaccaga tcccgcatat gtaaataacc cggtaatgtt gtggcgactg ccagacaaaa
    38701 tttcacatcg cgtgcggcgg ctttcgccag aacgtcatcc acgtccttat gcaaagattc
    38761 ataatccaga ccatcgagat ggcagtgtga gtcgactaaa aacatgatgt ctctcttaaa
    38821 gatgaggaac cggtagcaca acgcccggtt gcaggtaatg ctcaatacgc agtaaaagat
    38881 cggtgatgag aagctcgcgg ttgatgcctg tgacagacat taactgttca cgaatgtggc
    38941 aaacatcccc cagtatagcc tgcaggcgcg agggagaaag atggtttgcc agttcgacga
    39001 caaggcccgg cacatcaaca ttggtcacct gcgcagcacc atgatggcgt tttagcgcat
    39061 ccatcagcaa cgttgccagc cagtgtaaac gcgccggagc ttgttcatga ttaagggccg
    39121 ccagcagcga ataccagtcg cccgatggca cgctatatgc caatgcctga cacaatgttt
    39181 cacgagcctg ccagttatct ccctgaaaca acgccagtgc cgcgccaggc gaaccggcgc
    39241 ttaagcgcaa tgcggcaagt aatgcatcct gtgacattgt cacttcgcgt gaaagccagg
    39301 tcacggcgta ctgttccggc ggcggcgcaa ggtaatgtaa ccgacaacga ctacgtaatg
    39361 ttgccagtaa acgttcaggc tcgcgggtag ccaggaaaaa ccaggtttct gctggtggct
    39421 cttcaagcgt tttcagcaat gcgttagccg cggcgtcggt cagtaaggca gcatcggtta
    39481 cccagacaac tttcgcacca cctaggcgtg cgtgctcatt cagcttttcg gtgacctcac
    39541 gtactgcatc aatgcccagc gtatttttcc ctttttcggg agccagggtg tagtaatcgg
    39601 gatgcgtgcc agcctgcatc aactgacatc cacgacagtg accgcaactt ttgtggccct
    39661 gcggttgttg gcagagtaaa taacggctca gggcgtagat taaagcatca tcgcccatgc
    39721 ccggtaacgc ctgaatgagt agcgcatggt gacctcttcc ggcctgatag ctggctacca
    39781 gtttttcgaa atcaggtcgt aaccatggat accatctcat gcgtccaact ccttcaccca
    39841 gtgggtcacg gtagtgcgga ttgcatccat cacggcctcc agcggctggg tggcatcaat
    39901 ggtatgaata cttttatctt gtgcagccag ttccagatag cgggcgcggg tgcgattaaa
    39961 gaaatcgaaa gactcttgct caatacgatc cagctcgccg cgcgcacgcg cgcgttttaa
    40021 gccaacttcc ggggtaacat cgagatagag cgttaagtcg gggcgaaaat ccccgagaac
    40081 agcatcacgc agtgttgcca gcatatgttg gtcaatacca cgtcctccgc cctgatacgc
    40141 ctgagtggag agatcgtggc gatcgccaat cacccaggtg ccgttagcca gagctggttt
    40201 gatgaccgtt tctaccagtt gaacgcgcgc ggcataaaac atcagaactt cggctttatc
    40261 ggtaatgact tcatcgccta ccgatttgat atccagcacc aggcttctta acttttcggc
    40321 aagttgcgta ccgccaggtt cccgagtgaa aaccatgtcg cggataccca gttgctcaag
    40381 cgtctcaacc accacattac gcgcggtagt tttgcctgcg ccttccagcc cctcaatgac
    40441 gatatactta ctgcgcattt ttttccttaa gcactttcag ataatcctgc acagacttgt
    40501 tatggctggc aagattggta ttaaacgtgt gaccaccttt accatcggcc acaaaataga
    40561 gatacggcgt ttttgccgga tgcgcagcag ccttcagcga atccgccccc ggcgtagcta
    40621 tcgcacccgg cggcaaaccg gtaatggtat aggtgttata cgctgtcggc gtttccaggt
    40681 ctgcacgaga aagtttgcca ttataacgct ctcccatccc gtaaatcacg gttgggtcgg
    40741 tctgcaggcg cataccaatg cgtaaacggt tgataaatac cgaggcaacc tgatcgcgtt
    40801 cactggcaac ggcggtttct ttttcgataa ttgatgccat cgtcaccagc tgatttttat
    40861 ctttataagg cagaccgtcc gcacgccctt cccaggcgct atcgaccgct ttcaccattt
    40921 tcttgtgcgc tcgcttgagt aacgcgacat cggtggtatt ggcggtatac atccaggtgt
    40981 ctggccagaa ccaaccttca atccactccg ggttttccag ttcaagtgcc tgcgctacgg
    41041 tggcgtactt atcgtcactc agcgtgtgct tgatatacgg agcctcacgc aattgcttga
    41101 ggtagtcgct cagacgcatc ccttctacca gtcgcagcgg gaactgtgct tctttaccgc
    41161 tttccagcaa tttcagcatc tcgcgcacgg tcatctgcgg tgtaaagcgg taagtcccgg
    41221 ctttaaagtg agaaagatcc ggttcgatac gcagcaacca ttgaaaaacc cgtgggcgat
    41281 tgatgatctt atcggcataa agctgttcac caagcgccag acgtccggtc cctggcttca
    41341 gggtaaatat cgtctcttct ttgataagca atttgctgtc ggcaagatgg cgaaccttcc
    41401 agacgcccac accagcggcg atacccagta ccaccagcaa taacaagata atcaataaca
    41461 cttttttcat gactaattcg ggcgctcaca aagtggggct aaatattcat ataacgttgc
    41521 tgacgaaaag gagacatcgc cacaggcacg tacgggcatc actggcatta acgcattaca
    41581 aataaccatc tcatctgcct gcaacgcctc ttccagagag gcttgcactt cgacaagctg
    41641 ataagaggat tgtgccagca aacggataca gaattgtcgc ataatgccgt taacacctgc
    41701 ctgatccaga cgcggcgtat aaactacatt gcccttccgc cagaacaaat tagccgcaca
    41761 gcattccgta acccaccctt cgctgtcaag gaccagcgcc tcatcagcgt ttgtctgctc
    41821 aagatgagag cggatcaata cttgctcaag ccgattaaga tgtttaatac ctgcaagatg
    41881 aggattgcgc cccagccgca ccgggcttag cgccaacgtc atcccttcgt tacgcaaacg
    41941 gtcgtaatgt gcaggataaa ccgtaacgga gagaatccgc gttgccggtc cactgttcaa
    42001 tgtgctgtac cctcgcccgc cactaccgcg actgatcacg actttcagca caccattttg
    42061 ctgttctgct gccagcgttt tcatctcttg ttcaagctga ggccagaagt cacagaaaat
    42121 catcaaccgc tgacaagcat cctgtagtcg ctggatatgc gccgataaca aactgacttt
    42181 accgtcgata actctggcgg tggtaaaaca accatcacca aactgcgttg cccgatcgct
    42241 tactgccagc gattcctgct tatgaccgtt aattaagaac ataccggctc cttatcagag
    42301 aattgttagt gtggcagcat gttcactacg gaacaagtcg gaataaaagc taagaaaaaa
    42361 ggcccgcaag cggacctttt atatgggtgg gaaatgacaa cttagatctt tttaaagatc
    42421 aaagaaccat tagtgccacc gaagccgaag gagttacaca gcgtgtattc cattccgctg
    42481 acctgacgtg cttcatgcgg tacgaaatcc agatcgcaac cttcatccgg gttatccagg
    42541 ttgatggttg gcggaacagc ctgatcgcgc agcgccagga tggagtagat agattctact
    42601 gcacccgccg cacctaacag gtgaccggtc atagatttcg tggagcttac caacacacgg
    42661 cttgcagctt caccgaagat ggttttcacc gcctgcgctt cagctttatc gccagccggc
    42721 gtagaagtac cgtgcgcgtt aacgtagcca atctgactcg cttcaatgcc tgcatcacgc
    42781 agagcatttg ccatcgccag agctgcgcct gcgccatttt ctggcggtga cgtcatatga
    42841 taagcatcgc tgctcatacc aaagccgacg agttcagcgt aaattttcgc accgcgtttt
    42901 ttcgcgtgtt cgtactcttc aagtaccagc ataccggcac catcgcccag tacgaagcca
    42961 tcacgctctt tatcccacgg gcggctcgcc gcttgcgggt tatcattgcg ggtagataat
    43021 gcacgtgccg cgccaaaacc accaacaccc agcggcgtac tggctttctc tgcgccacct
    43081 gcaaccatca cgtcagcatc gccatacgcg ataatacgcg cagcatggcc aatgttgtgc
    43141 acgccggaag tacaggcggt cgcgatagag atgcttgggc cacgcaggcc atacatgata
    43201 gtcagatgac ctgccaccat gttcacaatc gttgacggaa cgaagaatgg gctgatctta
    43261 cgtggaccac cgttcatcag agatgtgtgg ttttcttcga tcagtccgag gccgccaatc
    43321 ccggagccaa ttgcggcacc aatgcgggtt gcgttctctt ccgttatatc aaggccagaa
    43381 tcctgcatgg cctgaacgcc agcgacaatt ccatattgaa tgaaggcatc catcttgcgc
    43441 tgttctttgc gcgagataat gtcctcacag ttaaaatcct ttactaagcc agcaaatttc
    43501 gttgcatagg cgctagtatc gaaatggtcg attaggctga tgccactctg accggcaagc
    43561 agagctttcc aggtagactc tacggtattg ccgacaggag acaacatgcc cagtccggtc
    43621 acaactacac gacgcttaga cacgtttgtc ctccagggag ggaaaaaatg attctagtgg
    43681 gacaaaaaga taaaactcag gcggtcgaac gaccgcctgg agatgttcac ttacgcctgg
    43741 tggccgttga tgtaatcaat ggcagcctga acggtggtga ttttctcagc ttcttcgtcc
    43801 ggaatctcag tatcaaactc ttcttccaga gccattacca gctcaacggt gtcaagagaa
    43861 tccgcgccca ggtcttcaac gaaagaagca ttgttggtaa cttcttcctg cttaacgccc
    43921 agctgttcgc cgataatttt cttaacgcgt tcttcgatag tgctcatact cttaaatttc
    43981 ctatcaaaac tcgctttcgc gatggttttc gtagtgtata aaatgttgaa aaatttgcaa
    44041 ctaaatcccg gcaggtctta ccacgatttt acgttatttt gaggccttac cccccaataa
    44101 cgcaaatcat tttgcactaa ttattactca taaccacgca gactgcgcgc aacatgagca
    44161 ttttgtgcaa atcgcggtca gaccatgtac atcccgccgt tcacatgcaa agtttcaccc
    44221 gtgatgtaag ctgcttcgtc ggatgccagg aatgcaaccg cgttggcgat ttcctgtgcg
    44281 ccgccgaggc gacccgcagg aacctgcgcc aggatacccg cacgctggtc atcgctcagc
    44341 gcacgtgtca tgtccgtttc aataaagccc ggagcaacaa cgtttacagt aataccgcgt
    44401 gacgcaactt cgcgcgccag tgatttactg aagccgatca agcccgcttt cgccgcagcg
    44461 tagttggcct gaccgccatt tcccatggta ccaaccacag aaccgatagt gataatacga
    44521 ccatgacgct ttttcatcat agcgcgcatt accgcttttg acagacggaa aacagatgaa
    44581 aggttggttt cgataatatc gttccactct tcatctttca ttcgcattaa caggttatca
    44641 cgagtgatac cggcattatt gaccaggata tccacttcac caaattctgc gcgaattttt
    44701 tccagaacag attcgataga tgccgggtcg gtcacattca acatcagacc tttgccgttg
    44761 gcacctaaat aatcgctgat cgcctgagcg ccattttcac tggtcgcagt gccaataact
    44821 ttcgcgccac gggctgcgag cgtttcagca attgcgcggc caattccgcg gcttgcaccg
    44881 gttaccagtg cgatttttcc ttcaaaattc atgattttcc tcttttaaag ctcaagcgcc
    44941 gctgccatcg ctgaaggttc gttcagcgcc gaggcggtca gggtgtcgac aatgcgtttc
    45001 gtcaggccag taagcacctt gcccgggccg acttcataga gatgttctac gccttgcgct
    45061 gccatgtact caacagactt cgtccactga accgggttat acaactgacg taccagtgcg
    45121 tcacggatgg catcaccatt ggtttcgcat ttcacatcaa cgttattcac aacagaaact
    45181 gttggtgcgt taaaggtgat tttcgctaat tctactgcca gtttgtcggc tgctggtttc
    45241 atcagcgcac agtgagacgg tacgctcact ggtaacggca gagcacgttt tgcgcccgca
    45301 gctttacagg cagcgccagc acgctcaacc gcttctttat gaccggcaat aaccacctgt
    45361 cccggagagt taaagtttac cggagaaacg acctgacctg ctgcagcttc ttcacacgct
    45421 ttcgcaatag acgcatcatc cagaccgatg attgccgcca tagcgcccgt accttccggt
    45481 acggcttctt gcatgagctt gccgcgcatc tcaaccagac gcaccgcatc agcgaaatca
    45541 atcacaccag cgcaaaccag cgcggaatat tcccccaggc tgtgaccggc catcattgcc
    45601 ggtgctttac cgccctgctg ctgccatacg cggtacagcg caacagaggc agtcaacagc
    45661 gctggctggg tttgccaggt tttattcagt tcttcagctg gcccctgctg ggtcagcgcc
    45721 cacaggtcgt agcccagcgc cgcagaagct tcagcaaacg tttcttcgac aattggatag
    45781 ctcgccgcca tatcagccag cattccaacg gtttgagaac cctgtccagg gaacacaaat
    45841 gcaaattgcg tcatgtttta atccttatcc tagaaacgaa ccagcgcgga gccccaggtg
    45901 aatccaccgc caaaggcttc aagcagaacc aactgccccg gcttaatgcg cccgtcgcgt
    45961 acagcttcat ccagcgcgca cgggacagag gccgcagagg tattaccgtg gcgatccagc
    46021 gtcaccacga cattgtccat agacataccg agttttttcg ccgttgcact gataatacgc
    46081 aggttagcct gatgcggaac cagccagtcc agttgagaac ggtcaagatt atttgccgcc
    46141 agcgtctcat caacgatgtg cgccagttcc gttaccgcaa ccttgaagac ttcgttgccc
    46201 gccatcgtca gatgaattga attctctgga ttcacacggt cagcattagg cagcgtcagc
    46261 aactcaccat agctaccgtc ggcatgcaga tgggtggaga tgattcccgg ctcttcagag
    46321 gcagccagca ccgcagcgcc cgcgccatcg ccaaaaataa taatagtccc acgatcggtt
    46381 ggatcgcagg tgcgcgccag tacatcggaa ccgacgacca gagcatactt caccgcccca
    46441 gatttcacgt attgatcggc tacgcttaat gcataggtga aacctgcgca ggctgctgca
    46501 acgtcaaatg ccgggcaacc tttaatgccc agcatgcttt gaatctgaca agctgcgctc
    46561 gggaaagcgt gcgtagcaga agtcgttgcc acaacgatca ggccaatctg gtctttatca
    46621 atgcccgcca tctcaattgc gcgtgtcgcc gcttcaaagc ccatggttga aacggtttcg
    46681 tttggcgcgg caatgtggcg ttcgcggata ccggtacgag tgacaatcca ctcgtcagag
    46741 gtgtccacca ttttttccaa atcggcgttt gtccgcactt gttcgggcag atagctgcca
    46801 gtaccaataa tcttcgtata catgtacgct cagtcacttt tcggttatat accgtcactt
    46861 gcaaactgcg agttcgctgg cagcgtcctg ctaccgcaga gttccgcttt tgccaccgtc
    46921 cagcagctca aaaccagctg ggtatacaga ttccaggcga gcggcaattc gctgaggaac
    46981 ttgtcgctgc accgcctgca ctgcctgttc aatcgcgacc gcaaaagctc gctgattggc
    47041 tgcaccatga ctttttatca ccgtgccgcg caatcctaac agacaggcgc cgttatactg
    47101 gtcggggttg aggtgactga atcgcctcgt caggctcttt tgtagccaac gctttaataa
    47161 cagtagccac cacgaccgtt ttttcccttc accctgagat ttcagcagag aaaggaacat
    47221 cctgacaaca ccttccatcg tctttaatgt gacatttcct gtaaagccgt cacaaaccag
    47281 cacatctgtc ttgccagtta acaactcatt ggcttcaaga tagccgatat aattgataga
    47341 agggattgtt ttaagcaccg ctgaggcatc ccgaatactg tcgagaccct ttacttcttc
    47401 ttcaccaata ttgagcaacg ccacgcgagg attgggaatt tccaccacct cttcagccag
    47461 aactgagccc ataatggcaa attgcaccaa cattgtactg tcacaatcga cgttggcccc
    47521 taagtcaagg accaccgttt tgcccttttg ctgatgtggt aataccgtca ccagcgccgg
    47581 acgctcaatc ccctccaggg gcttgagtaa taattttgcc agccccatca gtgccccggt
    47641 attaccggca ctgacacagg cttgcgctcg accttctttc accagctcca gggccacgcg
    47701 cattgaactc ccacgactgg cgcggatagc ttgcgaaggc cgggcatcac tggcgataac
    47761 tgactgcgca ggaataatct gcagacgcga acgttgttca aagtcagctt tagcaagtaa
    47821 tggcgtgatg gcgtcgggat tgccgactaa aagaagagtg agttgcgaat tagaattcag
    47881 tgcctgcaat gctgcaggca ctgtcacgga agggccaaaa tcccctccca tgacatctaa
    47941 cgccagggtt agacgtgtca aggtatcgtc gccgcccggt ttggtctttc cccagttgcc
    48001 tggggaaaat cctcactaag cttcatcacg cagacgcgtg attacttagc gatgaccttg
    48061 cggccgcggt agtaaccgtc ggcagtgatg tggtgacgca ggtgtttttc accagaagtt
    48121 ttgtctacag acaggctggt gactgcggtc agcgcgtcat gggaacgacg catgccacgt
    48181 ttggaacggg ttggtttatt ctgttgtacg gccatggacc ttactcctca attacggttt
    48241 atccccgatc caacggggag caccaattac ttacgcttta agctggctaa tacggcaaat
    48301 gggtttggct tttgcacttc ttcaggcagt tcaccaaaga ccatgtccgc ttcggacact
    48361 tcacagtgtt cagaatcatg caccggaact accggcaagg cgaggatgat ttcatcttca
    48421 accattgcaa gcagatcgat ttcaccgaat tcgttaacct caatcggttc atacgcttcc
    48481 ggcagtgctt cagcctgttc gtctgaacgc acaggactaa aacaatacgt tgtgtagacc
    48541 tgatgagtaa acggcttccc gcaacgctga cactcgagcg ttaccgtcac cttcgcatcg
    48601 ccgtttaaca ctgcgagacg ttggttatcg atagcgaacg acatggagca ttccacatca
    48661 ctgtccacac tgactacgga ttcggcgacg cgctcaacct gatcaggggt atagataccc
    48721 tggtaatcaa ggcgtttttg agccgtacga accggatcga gagtcagggg taattttacc
    48781 ttttgcatag ggcgcgcata ttaactttgt aacgtcatag agtcaaagaa aaaggcagcc
    48841 tgtggttgcc ttttgccaat aattcgcaca cattgcgggt tactgcttta ttttcgttca
    48901 gtgctgataa cgaacatcaa cctgcaaaac gaagcgcata gtttaaaatg gctatcatca
    48961 atacgctata tctgatggaa aaaaatatgc ctaaacttat tttagcctcc acctcgccat
    49021 ggcgtcgcgc tcttctggaa aaactgcaaa tctcttttga atgtgcagca ccagaggtcg
    49081 acgaaacccc acgcagcgac gaatcaccgc gacagttggt gcttcgactg gcacaagaaa
    49141 aagcgcaatc tctggcgtca cgttatccgg atcatttaat tattggttca gaccaggtat
    49201 gtgttcttga tggcgaaatc accggtaaac cgttaacgga agaaaatgcc tgtctgcaat
    49261 tacgcaaagc cagcggcaat atcgtcacct tctatactgg actggcgttg tttaattcgg
    49321 cgaatgggca tctacaaaca gaagtggagc cttttgacgt ccatttccgt cacctgagcg
    49381 aggcggagat tgataattac gtgcgtaagg agcatcccct gcactgcgcg ggtagcttta
    49441 agagtgaagg atttggcatt acgctgtttg agcgtttaga gggacgtgac cctaacacgc
    49501 tggttggttt accgctaatc gcgctgtgtc agatgttacg tcgggaaggg aaaaacccgc
    49561 tgatgggata gttgtttgcg ctggatgcgg ttacgtcgca tccggcaatc ggtgcaggcg
    49621 gcctgatgcg gcgtaaacgc cttatcaggc caaccgccca ttatcaggct tatatgcttg
    49681 tattagcgcg cgttacgcag cttttgcaaa caacgcttca aaccttcatc catcggcgct
    49741 tcgatacgca tcacctcacc ggtccccgga tgggtaaact tcaacgctgc ggcgtgcagg
    49801 aacagacgat ttaatcccgt gcctgcttca gtgagctgtc tgtcaaattc acggtcaccg
    49861 tagcgatcgt caaaggcaat cggatgaccc gcatactgtg tatgcacacg gatctgatga
    49921 gtacgtccgg ttaccgggct acaacgcacc agggtggcaa atgcatagcg ttcttccact
    49981 ttaaagcgtg tttccgacgg tttgccttcc tgactgacac gcacgatacg ttcgccgctt
    50041 tgcagaatat ttttcaataa cggcgcttga acgctcttca catgcgactg ccactgaccg
    50101 cgcaccagcg ccaggtaatc tttttgcatt cctttttcac gtaattgctc atgcagagaa
    50161 cgcaacgccg agcgtttttt cgctatcagc aacacacctg aagtgtcccg gtcgagacga
    50221 tgaaccagtt caaggaaccg cgcttccggg cgcaacgccc gcaaaccttc gataacgccg
    50281 aagcttaaac cactgccgcc atgtaccgcc gtaccggaag gtttattcag caccaggata
    50341 tgatcatctt catataagat gacgtccgcc agcgccgcca ccttttgcag atgtggcgaa
    50401 accgcctctt cttcccgctc agcaacgcga accggtggga tacgcacctc atcacccgct
    50461 tcgagtttat attcaggctt aatacgtttt ttgttcaccc gcacttcgcc tttacgcaaa
    50521 atacggtaaa tcatactttt tggtacgcct ttcaattggg tacgcaaaaa gttatcgata
    50581 cgttgccccg cttcgtcagc ggtgatagca acaattttta cggatggagt ctctgttttc
    50641 atggtgggcg attctaatta gccaacagga ttcgcgccac tcatttttct atgcttatat
    50701 ttagttttgc accttattac ttcactacgt gatcacttta ttgatggtta ttaaaccaat
    50761 caccagcaag aagtgaaaaa actgtgagta agcgggtgat aaatggtaaa agtcatcttg
    50821 ctataacaag gcttgcagtg gaataatgag gccgtttccg tgtccatcct tgttaaaaca
    50881 agaaatttta cggaataacc cattttgccc gaccgatcat ccacgcagca atggcgtaag
    50941 acgtattgat ctttcaggca gttagcgggc tgcgggttgc agtccttacc ggtagatgga
    51001 aatatttctg gagagtaata cccagtctgt ttctttgata attgcgctgt ttttccgcat
    51061 gaaaaacggg caaccgacac tctgcgcctc tttgagctga cgataaccgt gaggttggcg
    51121 acgcgactag acacgaggcc atcggttcac acccggaaag gcgttacttt gcccgcagct
    51181 tagtcgtcaa tgtaagaata atgagtaagt tacgatgaaa agaatgttaa tcaacgcaac
    51241 tcagcaggaa gagttgcgcg ttgcccttgt agatgggcag cgtctttatg acctggatat
    51301 cgaaagtcca gggcacgagc agaaaaaggc aaacatctac aaaggtaaaa tcacccgcat
    51361 tgaaccgagt ctggaagctg cttttgttga ttacggcgct gaacgtcacg gtttcctccc
    51421 actaaaagaa attgcccgcg aatatttccc tgctaactac agtgctcatg gtcgtcccaa
    51481 cattaaagat gtgttgcgtg aaggtcagga agtcattgtt cagatcgata aagaagagcg
    51541 cggcaacaaa ggcgcggcat taaccacctt tatcagtctg gcgggtagct atctggttct
    51601 gatgccaaat aacccgcgcg caggtggcat ttctcgccgt atcgaaggcg atgaccgtac
    51661 cgaattaaaa gaagcactgg caagccttga actgccggaa ggcatggggc ttatcgtgcg
    51721 caccgctggc gtaggcaaat ctgctgaggc gctgcaatgg gatttaagct tccgcctgaa
    51781 acactgggaa gccatcaaaa aagccgctga aagccgcccg gctccgttcc tgattcatca
    51841 ggagagcaac gtaatcgttc gcgcattccg cgattactta cgtcaggaca tcggcgaaat
    51901 ccttatcgat aacccgaaag tgctcgaact ggcacgtcag catatcgctg cattaggtcg
    51961 cccggatttc agcagcaaaa tcaaactgta taccggcgag atcccgctgt tcagccacta
    52021 ccagattgag tcgcagatcg agtccgcctt ccagcgtgaa gtccgtctgc cgtccggtgg
    52081 ttcgattgtt atcgacagca ccgaagcgtt aacggccatc gacatcaact ccgcacgcgc
    52141 gacccgcggc ggcgatatcg aagaaaccgc gtttaacacc aacctcgaag ctgccgatga
    52201 gattgctcgt cagctgcgcc tgcgtgacct cggcggcctg attgttatcg acttcatcga
    52261 catgacgcct gtacgccacc agcgtgcggt agaaaaccgt ctgcgtgaag cggtgcgtca
    52321 ggaccgtgcg cgtattcaaa tcagccatat ttctcgcttt ggcctgctgg aaatgtcccg
    52381 ccagcgcctg agcccatcac tgggtgaatc cagccatcac gtctgcccgc gctgctccgg
    52441 taccggtacc gtgcgtgaca acgaatcgct gtcgctctct attctgcgtt tgatcgaaga
    52501 agaagcgctg aaagagaaca cccaggaagt tcacgccatt gttcctgtgc caatcgcttc
    52561 ttatctgctg aatgaaaaac gttctgcggt gaatgcgatt gaaacgcgtc aggacggtgt
    52621 tcgctgcgtg attgtgccaa acgatcagat ggaaaccccg cactaccacg tgttgcgcgt
    52681 gcgcaaaggg gaagaaacgc cgaccttaag ctacatgctg ccgaagctgc atgaagaagc
    52741 gatggcgctg ccgtctgaag aagagttcgc tgaacgtaag cgtcctgaac aacctgcact
    52801 ggcaaccttt gccatgccgg atgtgccgcc agcgccaacc ccagctgaac ctgccgcgac
    52861 tgtcgtagcc ccagcaccga aagctgcaac ggcaacacca gcagctcctg cacaacctgg
    52921 gctgttaagc cgcttcttcg gcgcactgaa agcgctgttc agcggtggtg aagaagccaa
    52981 accgaccgag caaccaacac cgaaagcaga agcgaaaccg gaacgtcaac aggatcgtcg
    53041 caaacctcgt cagagcaacc gccgtgaccg taatgagcgc cgcgacaccc gtagtgaacg
    53101 tactgaaggc agcgataatc gcgaagaaaa ccgtcgtaat cgtcgccagg cacagcagca
    53161 gactgccgag acgcgtgaga gccgtcagca ggctgaggta acggaaaaag cacgtaccac
    53221 cgacgagcag caagcgccgc gtcgtgaacg tagccgccgc cgtaacgatg ataaacgtca
    53281 ggcgcaacaa gaagcgaagg cgctgaatgt tgaagagcaa tctgttcagg aaaccgaaca
    53341 ggaagaacgt gtacgtccgg ttcagccgcg tcgtaaacag cgtcagctca atcagaaagt
    53401 gcgttacgag caaagcgtag ccgaagaagc ggtagtcgca ccggtggttg aagaaactgc
    53461 cgctgccgaa ccaattgttc aggaagctcc agctccacgc acagaactgg tgaaagtccc
    53521 gctgccagtc gtagcgcaaa ctgcaccaga acagcaagaa gagaacaatg ctgataaccg
    53581 tgacaacggt ggcatgccgc gtcgttctcg ccgctcgcct cgtcacctgc gcgtgagtgg
    53641 tcagcgtcgt cgtcgctatc gtgacgagcg ttatccaacc cagtcgccaa tgccgttgac
    53701 cgtagcgtgc gcgtctccgg aactggcctc tggcaaagtc tggatccgct atccaattgt
    53761 acgtccgcaa gatgtacaag ttgaagagca gcgcgaacag gaagaagtac aagtgcagcc
    53821 gatggtgact ggggtccctg tcgccgccgc tgtcgaacct gttgttagcg caccggttgt
    53881 tgaagaagtg gctgaagttg tagaagcccc cgttcagatt gccgaaccgc aaccggaagt
    53941 ggttgaaacg acgcatcctg aagtgattgc cgccgcagta actgaacagc cgcaggtgat
    54001 taccgagtct gatgttgccg tagcccagga agttgcagaa cacgcagaac cggtggttga
    54061 accgcaggaa gagacggcag atattgaaga agttgccgaa actgctgagg ttgtggttgc
    54121 tgaacctgaa gttgttgctc aacctgccgc gccagtcgtc gctgaagtcg cagcagaagt
    54181 tgaaacggta gctgcggttg aacctgaggt caccgttgag cataaccacg ctacggtgcc
    54241 gatgacgcgc gctccggcac cggaatatgt tccggatgca ccgcgtcaca gtgactggca
    54301 gcgccctact tttgccttcg aaggtaaagg tgccgcaggt ggtcatacgg caacacatca
    54361 tgcctctgcc gctcctgcgc gtccgcaacc tgttgagtaa taattagctc aagtaatcaa
    54421 gccctggtaa ctgccagggc ttttttattt catctttgaa tcaacatatt aaaattcatc
    54481 gaatggcatc cttgctaacc aacaatgcaa aataggcaaa aaaatgcccg ccccggcgat
    54541 cccagagcag gcagacaaaa catacccagt ttcatgatat gttttaaagc gaaattattt
    54601 gttgagctgg aagagcgaca atccctgcat atcggtaaat gctttatacg atgcctgcaa
    54661 tgccgtttgc tgcatgatgt aagatgaaat agttgcattc cagtccacat caaccagatc
    54721 gctcatctgc tgcgtttgcc ctaaagcgcg atcgctacct aatgaatcca gcgactccag
    54781 ttcgttcagc tgcgtgccta attccgcgcg aacggtcagc acattgttca gcgagttttt
    54841 cagtccgcgg ttggttttat ctaacgccgc agcggcggtt tctttatccg cttcgctatc
    54901 cgcgaccggc gttttcaggg ctgcgatggc gctatccagc atggcaaaaa gattggtttc
    54961 agaagcgcta ccgtctggtt ccgctaccgc gttactggta atactgtcga aaattttgtc
    55021 gcccgtatgt cctatcacca tcgaacgcga agcatcgacc tgttgcttaa tactttctgt
    55081 tccgccgaca taatcaccgt tagcctcact aaacggcgca gtctctgttt tataaccggc
    55141 aaaaatgtag cgcccattac cgtcagtggt gttcgccaga ttcagcaact ggtcacgaag
    55201 cccctgaata tccgtagcca gcgaggcccg gtcatcgtca ctcaaggtgc cattgctggc
    55261 gtagacaatt ttttcctgag cattctggat agcagtggtg acctggctaa gtacactctc
    55321 ttccagtgac actttttgag tggcgaaagt acgcgccagc gtgtactggc tgttttgcgc
    55381 ctgtgcctgg gagagaacaa ccgcttgtga tgcagcaatg ggatcatctg aagggttaac
    55441 gactcgctta cccgtcgaca tctgttcgcc gtacttcatc cattctgcct gagaattggt
    55501 gataccacgc atgttttgct ggtacatcat ctgggtactg aaacgcattt tatctcccct
    55561 tagcgaatgt taatcagcgc atcaaaaatc gcgtttgccg tctgcagaac ctgcgcattc
    55621 gccaggtaat actgctgaaa acgttgcaga tttccgtact cctcatcgag attgacaccg
    55681 gaaatcgact gctgctgatt ggaaagctgc gtcaccacat taccttgcgt ggtgctactg
    55741 gttttcaacg tcgcggtttt attaccgata tcactcacta acgaggcata agcgtcgtta
    55801 aaggatttcg caccgcccac cgttttactg ttgctttgca gatccagcag ggcctgaccg
    55861 ttgcggttat cgctatcacc cgcatctttt tcgctcgcca tcgctatttt cgcttcgtcg
    55921 gtgattaata catccatgtt gacgatggcg tcacttactg gtttcagcgt gaagctgtcg
    55981 ttaacggcag gcgttcctgt aaacgtcaac tccagaccat caaatgccac tttaccgttg
    56041 gcatccggcg tcaccgtaaa agtggtattg ctggcaaggc gggtgacctg ccactgatta
    56101 ttatcgaacg agattttgta atctgtcgcc agtaccacgg aggcatcagt taccgtggca
    56161 ccgatcgcaa cgtcaccttt gtttttcgtg ttttgcagaa ccgcgggctt accgatagta
    56221 aagaaatctt caccagcatc gccgttagca tcaaatccgg ctttgtgttg ggtgttgaaa
    56281 gcctcggcaa atgccagcgc cagttgtcca agcgtattac gcgtctggtc cagctcctga
    56341 gaacggaatg tcagaatgcc gcccagcgac ccggtattca gtaatttctc cgggatctca
    56401 atattgcctg ccgtcccatc aacataagcg acagtcgtac gagaagggtc agcgctggaa
    56461 ggaactgccg ccagttgccg cgccgtactt ccctgaacca gtgagtaacc attggccacc
    56521 gtgatgttat aagtgccgcc atcctgaacg ctgacttcta caccaacaat ctggtttaat
    56581 tcgctcacca gttgatcgcg ttgatccagc agattgttag gtgacgcccc tgcccccacg
    56641 cctgtcaggc gcgagatttg atcgttcagg ctggcaattt gtttagcgta attgttgatc
    56701 tgatcaacgc tggcacctat cgcgatattg acctgtttgt cctggtcgcg cagatattga
    56761 tcggtggttt taaactgatt caccaaccct tctgatttcc caatcagcgc ctggcgcgct
    56821 gccgggtctt ccgcgttact caccagcgtt tgcaggctgg tgaagaaatc ctgcatctgt
    56881 gttgccagcg aagaggtact ggtggagagc atattgtcga ttttcgacat ctgctcatag
    56941 cgggcggtca ggccgctact ttgcgtctgc gcagcacgta actggttggt aataaacgca
    57001 tcatactcac gctgcacacc agaaacgtag acgccattgc caacccagcc gccagcgccc
    57061 aacgtgctat tggcctgcgc cataatagtg gtttggcggg tatatccggc aacgttatag
    57121 ctggagatat tattacttgc cgtatttaac gccgcctggg ccgcgttcag tccgctcatg
    57181 gcgttattga tcaagctgga catggaggtt ccttataagc cttcaattac agagtattat
    57241 cggcagcgac ccgccggact tgagttattc agaacagatt atcaatgttc atgctgtagg
    57301 ttttgctcac cttgtcgctt atcgatttca tctgctggat catgttggtg agtttacggg
    57361 catagtgagg atcggtagca taccccgcgt cctgtagggc ctgcgccccc tgctccgcac
    57421 tcgcggctgt cgtcacggcg gcgtagcgcg ggttacgtgt taacagccca acgtaatccg
    57481 acaatgcttc cagatacgag ctgtagaccc gaaatttcgc ttttactttc ttcgcttcgc
    57541 cattttcata ttcagtcgtg gtgatttcag taactggacc tttccagttg ccagaggctt
    57601 tgacgccaaa caggttatag ctcggctcgc cgttttcgcg gcggatttgc cgttgccccc
    57661 aaccagactc cagcgccgcc tgagcgagga tcaaatgatg tggcacaccg ctttgctggc
    57721 ttgccagttg ggcgggcagc gagagttgcg cgaggaatgc tttactgtca cccggcagcg
    57781 aatcatcgta gttacgtggc acggcctttt gcaccagctg cgaaagcgcc tgattttgat
    57841 aacgcaccac agtttcgagc gggaatttca tcggtgctgc tggcgtggac tcctctggca
    57901 atggttgttc tggcgtcatc tgtttaacca tcatctctgc aagccccaga cctttgcccg
    57961 ccgtcatctg ttgggcaatc tgctggtcat acatactggt atacaggcga gtgtgctcgc
    58021 tgctgaacag gccatctttt ggtaaagcgt cgcgcatgct tttcaacatc atctgcacga
    58081 acatcccttc cacctgacgg gccaccggac ggatatttgc cgccggatct tcgcccgctt
    58141 tcgcctttag ttcgttgagt gattgcgcat cccaggccgc acttgccagt agtttgctgt
    58201 cgctgatcat cagatgattt ccagttttgc ccgcagacat cccgcacttt gcattgattg
    58261 cagtatggac atcagatcca tcggcgtagc gcccagcgca ttgagcgcgc gcaccacgtt
    58321 attgaggctg gcgctggaac gtacgctttg cagcgaaccg ccgctctggc gtaaatcgat
    58381 ctgcgtttgt ggagtaacca cagtctgtcc accaccaaac ggtgtatctg gctggctgac
    58441 attggcctga cgattgactg ttactgagag atttccctgc gctaccgcgc agctgtcgag
    58501 ggtcacttcg cgattcatca ccaccgaacc ggtgcgcgag ttaatcacta ctttagcgtc
    58561 ctgcggggtg acattaacct gcatattctg gatatctgca aggaaacgga cctgggaact
    58621 gttgccactt ggtacgcgca cctgaatagt ccgcgcatct aacgcggtgg cgctgccata
    58681 tccacgcacg cggttgatgg tgtcagcgat ttgctgcgcc atgctgaaat cttcgtcgtt
    58741 aagttgcaaa ttaagggtat tcccgacgcc aaactggctg ggcaattcac gttcaataac
    58801 cgcaccattg gtgatccgtc caccgttcag ttggttcacc tgaacactgc taccgccagc
    58861 ggaggctcct gcgccgccaa ccagaatatt gccctgcgcc agcgcataca cctgactgtc
    58921 aacgccctta agcggcgtca tcaacaacgt acctccacgc aagcttttgg catttcccat
    58981 ggaagaaacc accacatcga tggtttgccc ctgacgtcca aacggaggaa gtgactctgt
    59041 caccattacc gcagcgacgt tttttagctg catattggtg cccgtcggaa cggtaattcc
    59101 cagctgtgag agcatgttat taagcgtttg tgtggtaaac ggcgtctggg ttgtctggtc
    59161 accggtgcca tccagcccca ccaccagacc atagccaatc agtgagtttt gccttacccc
    59221 ctgaacactg gtgagatcgc gaatacgctc agcctgagcc gccgtcgtaa ccagtagaag
    59281 aattaatgca gagagaaatt taatcaccac agcctcactt acattggcga caggttaagg
    59341 aagaaacgct gcaaccagcc catattttgc gcttcgttaa tgtagccatt gcctacgtat
    59401 tcaatgcgcg catccgccac ctgagtagac ggtacggtat tgctgccgct gatagtgcgt
    59461 ggattaacca cgccagagaa gcgaataaat tcggtaccct gattaatggc aatctgtttt
    59521 tcacccacca catgcaggtt gccgttgacc agtacctggt caaccgtcac cgtcaacgtg
    59581 ccgctaaagg tattgctggc attggccccg ccctttccgt tgaacgtgtt accaccggag
    59641 gcttcgacat cggcacgagc gttaccaaac agcccctgca aatagcgcgg cacagtatca
    59701 aagccaaaat tagttttacc gtcacggctg gcattcgcag aggagctttt gctggcgctg
    59761 acgttctcct gcaacacgat ggtcagcgta tcgccaatat tgcgtggtcg acgatcttca
    59821 aacagcggtt gatagccata gttaatcggc tgagcagact ggaaaataga accgttggcg
    59881 acgggcgtcg gaccgggaac cggttgtgca ctggtcgccc cctgcaccag cggcgtggag
    59941 ggtatccagg cgcagccggt tagtgaaagc accaacaagc tggaaatggc ataagtatgc
    60001 gcagcgtttt tttgcatggc tatcatcttc aaaaatcagt aaaccggtga acctgccacc
    60061 ggttaagcct tagagttgcg tcagtttttg cagcatctga tcggtggtgg acaccgcttt
    60121 actgttgatt tcgtaagcgc gttgcacctg aatcatattg accagttctt ccgccacgtt
    60181 gacgttagac gtttcaacat acccttgata cagcagtccc gcgccgttca ggcccggcgt
    60241 gctttcgttc ggtacaccag aggattgcgt ttcggtgtag aggttttcgc caatgctctc
    60301 cagcccggtg tcattcataa aggtggtgag attgagctgc ccaacctgaa ccggagctgc
    60361 ctggccttgt tgggttacgc tgaccacgcc atcacgaccg atggtgatac ttaacgcatt
    60421 cgccggaatg gtgatcgctg gctgcacctg aaaaccacca gctgcccgtt ctgatccacc
    60481 tggaaagagc cgtcacgggt ataggctgat gaaccatctg gcaacatcac ctggaaaaag
    60541 ccctgccctt taatcgcgac atctttgctg ttgttggtct gcgacaggtt tccctggctg
    60601 tgtaagcgtt cagtggcgac cgggcgtacc cccgtgccga tttgtaatcc ggagggtaag
    60661 gtggtttgtt cggaagactg tgcccccggc tggcgaatgg tttgataaag cagatcttca
    60721 aacaccgcgc gctgacgctt aaaaccgtta gtactgacgt ttgccaggtt gttggcaatg
    60781 acgtccatat tggtttgctg ggcgtcaagg cccgttttgg cgatccataa tgaactgatc
    60841 ataaaatgtc ctgtagtgga ttagtgacaa agtgcatatg tcttcttgcc ggatgcggcg
    60901 taaacgcctt atccggccta caagtgctcg caaaatcgat aaattgcgat tgcccccata
    60961 gacccgataa acttgcgcat cgggcaactt atacttgtca tgtatccttt caattaactc
    61021 atcgacagca gttggttggc acggcctgcg ttatcatcga cgctgctgat caccttcatc
    61081 tgcatttcaa aacgccgcgc gctggcaatc atgtcgctca ttgccgcaac ggcattgacg
    61141 ttactgcctt ccagaacccc cgacatcaca cgcaaggttg gatctgcctg cagtaccggc
    61201 cctcgcgtgg cctgggtttc tgcgcttaaa cgaaaaatgc cgtcgtcacc gcgctgcact
    61261 tcgctgcccg tggctttcac cagtttcaga cgccctactg gcgcaactgt atttgccgga
    61321 tcgcccggat tgagcgccga gattgtgcca tcggcagcaa tagtgatttc cgctccttcc
    61381 ggcacagcaa ttggccccgc ctcgcctatc acctgatgcc cctgaattgt cagttgcccg
    61441 gtgggatcaa cctgaatgct gccattacgc gtataccctt cgctgccgtc agcggtctgc
    61501 acggccagcc agccatcctg ctgcaacgcg acgtccagcg ggcgcgaggt gtaatccatt
    61561 ttgccgggcg tcatatctgc gcccggcgtt gacgccgtga ccaacgtgcg cgtgggcaga
    61621 gaaagccctt ccactggcac cgcgcgtaaa gcattcaact gcgcgcgaaa accgggcgtt
    61681 gaggcattgg ccagattact ggcggttacc gcctgttgat tcagtgtctg gctggctgct
    61741 cccatcgcgg tataaattgc gtgatccatt gagctatccc gtcagcgatt agcgtaagtt
    61801 aaccagcgtg ttgaggatct ggtcctgagt tttgatggtc tgggcgttag actgatagtt
    61861 acgctgggca acgatcatat tgaccagttc tttactgaga tcgacattgg acgcttccaa
    61921 cgcaccgttg gtcagggtgc caaagttgcc cgtcccggct gtccccaaca gcgccacgcc
    61981 agaagattgc gtcgcagacc agacgttgtc gccttcggat gccagacctt cgttgttggc
    62041 aaagttcgcc agtacaatct gccccagcag ttgtgtttgt tcgttggaat agttgccgac
    62101 aaccgtaccg tcatcattga tttgataact caccagatcg cccggtttgt agccattctg
    62161 ggtggttgcc acaatattgt tagcgccggt attttgctgc atggagttga ggaagctcag
    62221 gctaaacgtg gcgggatctg cgccgttaat tgcgccagtg gtaatattct ctgttggatt
    62281 tgaggtcaga acgccattgg cattaaacac cagcttcatt gcaggctcgg ctgtacctgt
    62341 tggatcactg ctatcctggg tgtagacctg ccagttatta tccccggtct tcacaaagta
    62401 gacgctcatg tcatgagcat taccctgact gtcgaaaaca gtcaccgaac cttttttgtt
    62461 atagctatcc gcattgctgg cgctgaatgg cgtaacagta ggaagcggat cactggaatt
    62521 caggttgatc tgcatcgacg ccgtggtggt agttttcgct gccatcaggg tattcgggat
    62581 cgaaatattg gtcggattcg ccccttgctg aatagtcggc ggcgtaccgg ttgccgggta
    62641 acccgtcagc tgtaaacctt gcatattcac caggttacgg ttttcatcca gcttaaattg
    62701 tccgttacgg ctgtagaaca ccgaaccgtt gctgtctacc agacggaaaa aaccgttctg
    62761 gctgatagca acgtccagac ctcgcccggt gttggtggtc gtgccatcgg taaagtcctg
    62821 agtgataccg gcaactttta cccccagtcc cactttcgaa ccggcaaaca tatcggcaaa
    62881 agaggccgtg cctgatttaa agccgtaggt ggcggagttg gcgatattgt tgccaataac
    62941 atcgaggttg gtggcggcag cgtttaatcc gctaaccgct tgagaaaagg ccatgactga
    63001 ctcctgaaat gtgaaggctt aaattatctg ccgtacttcg tcgagggtgg tggtgccgta
    63061 agtgccgaga tccagcgtat taccgctgtt gccgcggatc acaccctgca ccagagcaaa
    63121 ctgcagcggc tgggcaacca gttgtgtacc accgttactg gcgctaatcg ctacattgta
    63181 agaaccgttc ggcgcagtgc tgccatcagt caacgtaccg tcccaggtga aactgtggac
    63241 tccggcggtc agttcaccaa tatcaatggt gcgcacaacc gcgccatttt tatcggtgat
    63301 ggtggccgta actttgtctg ccgcctgttg cagctcaaca ccaaacggcg tggtcgtggt
    63361 cacagcccct tcttcactgc cggttcccgc aagaacagtg gtgccgggga tcatcacgcc
    63421 gtgaccgatc aggttactgg cctgtaacga ctggctgtta tcaatctgtc cggaaataga
    63481 tccgagcgtg gtattgagtt tttcaatccc gctgaccgtg ctgatttgtg ccaattgcga
    63541 cgtcagctcg ttgttttcca ttggattggt cgggtcctgg tttttcagct gcgccaccaa
    63601 caaagtcaga aaactgcttt gtaagtctgc ggcgttgctg cccgtgagcg aactactgct
    63661 ggtggtactg acgccggtat ttgtcggatc ggtggtggtt accgcaatgg acatagcttt
    63721 ctcctttatt gaccgagcgt aagggttttc agcatcatgc ttttcaccat gttgagcact
    63781 tcaacattgg cctgatagct gcgtgacgcc gacatggtgt taaccatctc cccgacgaca
    63841 tcaacgttcg gcatttttac gtagcccttt gcatccgcca gcggattacc cggttcataa
    63901 accagtttgt ccggggcctg actttctata acatcggcaa cctttacgcc gcctgtcgca
    63961 gcacctggtg cagcgttaac ctggaatacc acctgttttg cccgatatgg ctgtccatcg
    64021 ggaccggtca cgctatcagc attcgccaga ttactggccg ccacgttcaa gcgctgggac
    64081 tgggcagtta acgccgaccc ggcgatatca aaaatattca gcagtgccat ccgttaattt
    64141 ccgctctgta aaacgttcat catgcctttg atttgcccgc tcaacgcgct aaggctcatc
    64201 tggtattgca ggctgttatc ggcaaactgg gtgcgttcgc gatccatatc gacggtatta
    64261 ccgtcaagcg aaggctggtc cggaatacgg tattgcagtt ctgcggtagg aggcgtcagc
    64321 gcctgcgccg gaatgtgttg cgttgaggtc atcgtcagtg caaccacact ggttgcatcc
    64381 cgtccacgtt gcatgacttt tttaagttca ctggcaaaat cgatatcgcg cgcctgataa
    64441 ccaggggtat cggcattggc gatgtttgct gccagcactt cctgacgctg ggcgcgcaga
    64501 ttgagcgcct cttgttgaaa acgtaaggcg gcgtcgagct tatcgagcat atctcctccg
    64561 caggtatcaa aattctgcca tcagcttaaa tgcctttacg cgcgcgttat cggcggaata
    64621 aacgcaaaat gggtcgctat ttatgccgtt gatgaccatt gcggacaggt acaattcacg
    64681 ttgtagaaat ggctgggggc gaaaatgctg gcaataaaac gtagcgtggc gatcatctcg
    64741 atactattca ctccgttaag tgcggcgagt aatctcacat cgcaattgca aacctttttt
    64801 agcgcccagc tcgcgggggt aagtgatgag gttcgtgttt ctattcgtac agcgcccaat
    64861 ctactaccgc cgtgcgagca gccattgctt tcgatgagca ataattcccg cctgtggggc
    64921 aatgtgaatg tgttggcacg ctgcggtaac gacaaacgat atttacaggt taatgtacag
    64981 gccacaggaa attatgtggt tgccgcgatg cccattgtgc ggggaggaaa gctggaagct
    65041 ggcaatgtca aactaaaacg cggacggcag gataccctgc caccgcgtac ggtgctggat
    65101 atcaatcaac ttgttgatgc cgttagcctg cgcgatctat cacccgatca acctatccag
    65161 ttaacccagt ttcgccaggc atggcgggta aaagcgggac aacgcgtcaa cgtgatcgcc
    65221 agcggtaacg ggtttagcgc caacgcagaa ggtcaggcgc tgaacaatgc agccgtcaca
    65281 cagaatgcgc gggtgcgcat ggtatcggga ctggtggtca gcggcgttgt tgatgcagat
    65341 gggaatattc ttataaacca gtaagctgtt aaagattacc cgtcccttgc cgataaataa
    65401 gcaacacatg ataaaagcgc cctcaatgag gaataaacca tgagtattga tcgcacttcg
    65461 cctctgaagc ccgtaagcac cgttcaacca cgcgaaacca ctgacgcgtc ggtaacgaac
    65521 acccgggcgg caaaaacaac agcctccacc agcaccagtg tgacgttaag cgacgcgcaa
    65581 gcaaaactga tgcaacccgg cagcagtgat atcaatcttg aacgtgtcga agcgttaaaa
    65641 ctggcgattc gtaacggtga actaaaaatg gacaccggca aaattgccga tgcgctgatc
    65701 aacgaagcgc agcaagactt gcagagtaac tgatcgtatg acacgtcttg cagagatcct
    65761 cgaccagatg tccgctgtgc ttaacgatct caaaacggta atggatcaag aacagcaaca
    65821 tctctctatg gggcagatca acggcagcca gttgcaatgg attacagaac aaaaaagctc
    65881 actgctggcg acgctggatt acctcgaaca gttacgcagg aaagaaccca atacagcaaa
    65941 tagcgttgat attagtcaac gctggcagga aattactgtg aaaacgcagc aactacgcca
    66001 aatgaatcaa cataacggct ggttactgga aggacagatt gagcgcaatc aacaggcgct
    66061 ggaaatgttg aaaccgcatc aggaaccgac gctatatggg gcgaacggtc agacctcaac
    66121 aacccatcgc ggcggtaaaa agatttcgat ctgaatatta tctccggcct gcactgcagg
    66181 ccggaatgca ttgttacacc gtccggcggg caaattcttt aactttgaag cccagtaccg
    66241 ccagtgcagc gaagtacgcg gcaatccccg ccagcacgac cgccattaaa cgcagtaagc
    66301 gccaaggcat ggtacccaat gaccactccg gcatgatatg taacatacct aaaagcacgc
    66361 cagacatcac cagtaccgcc accaccagac gcaacagaaa cgccatccag ccgggttgcg
    66421 gagtaaagat tttctgctta cgcaactgcc agtaaagcag cgaagcattc agacacgccg
    66481 ccagaccaat agaaagtgac agcccggcat gtttcaacgg accaataaac gccaggttca
    66541 tcaattgcgt taaaatcagc gtaacgatgg caattttcac tggcgtttta atgtcctggc
    66601 gggaataaaa gccaggagcc aacactttca ctacaatcag gccaatcaaa cccaccgagt
    66661 aggcaattaa cgcccgctgg gtcatcagcg catcaaacgc ggtaaattta ccgtactgga
    66721 acagcgaaac ggttaacgga cccgaaagaa tgcccaacgc aaccgcactc ggcagcgcca
    66781 acaggaaaca aagacgcaac ccccagtcca tcaaacggtt gtattcatca tgattgccac
    66841 tggcaaaact tttcgacagc gacggcagca aaatggtgcc aagcgccacg cccagcacac
    66901 cggagggaaa ctccattaag cggtcggcgt aatacatcca agacaccgaa ccggaagcaa
    66961 gaaacgaggc aaaaatggtg ttgatgatta aggagatctg gctcacagag acgccaagga
    67021 tcgccggtcc catctgtttc accacgcgca ttgctccggc atcgtggaag ttaatgcgcg
    67081 gcaggaccag catgccgatc ttcttcaggt gcggtagctg atacaccagc tgcaggatgc
    67141 cgccgaccgt aacagcccac gccagcgcca gcaccggtgg gttaaagtac ggtgcggcaa
    67201 acagcgcgaa accaatcatg ctgatgttaa gcagtgttgg cgcaaacgcc ggaatcgaga
    67261 agcggttcca cgtattcaga atcgctccca ccagcgacgc cagggagatc agcaagatat
    67321 agggaaaggt aatctttagt agctggctgg tcagggcaaa tttgtcagct gtgtcagcga
    67381 agcctggcgc ggtcaccatg atcacccacg gtgcggcgag catgccagcg accgtcacaa
    67441 ccgccagcgc aagtgtcagc aggccagaaa cataagagac aaagacccgc gtggcgtctt
    67501 caccctgctt acttttatat tccgccagaa tcggtacaaa tgcctgggaa aatgcccctt
    67561 cggcaaagat acggcgtaac aagttaggaa gtttaaaagc gacgaaaaag gcgtcggttg
    67621 ccatccctgc gccaaagatt ctggcgacaa ttgcgtctcg tgcgaagcca agcacacgcg
    67681 aaaacatggt catcgagctg acggcggcca gcgattttaa taaattcatc ggtgttctaa
    67741 tccagaccca cgggcaaaac gcctgcaagg caggcgctga aagaggcgaa tagtctacct
    67801 ggattatggt gaattgctac cgccagatgt tacagggttt attcactcat cgcatcgcgc
    67861 cagatcttgt caacgatacg ttgcgccagc actgcctgtt cgccggcggt ttgcagaact
    67921 gtctgatttt gcacacattc aatgaagtgc cgcgcacagc cgacaaatcc acgttgctca
    67981 agagtgctct gccaaccagg aatcggttta tgcactacgc cctgcccgcg ctcctcacgc
    68041 cattcgcgca tatccgtaat gtcgatgagc gcaccgtcag tcacggcctg cacggtttca
    68101 cgctgacttc cggcacggcg atgcatacag gtggtgatct gcaaaggatc ggccgaaaaa
    68161 tggtgctcgg caaacagcat ttcgccagcg tcgttagtca gtagcgtacc gccatccaga
    68221 gaggctttgc cgcccgacaa ccacagcgcg gtatccacca catgcagata atcatccagc
    68281 aacgtgaaat aaagatcgtg tggcccgaca ctattgctac gatgtttatc cattcttagc
    68341 gaagctgcgg tagcgagttg cgtttttaac tcaccgtaga gtggtgcgaa acgacggtta
    68401 aaaccgacca tcaacgtcag ttttttccgc gccgccagtt ccaccagccg ttcagcatcg
    68461 cgcagatttt ctgccagcgg tttatcgaca cagacatgta cacccgcatt gagtaacgta
    68521 ctgaccacgt caaagtggct ggcggtgctg gaatgcacaa aaaccgcatc gcaactggcg
    68581 gcaaggctgg ataacgaatc ggcataagga atgcgccagc tttcacaaat tggcagggct
    68641 ttcgcgcgcg taggcgacca ggctccttgt aacatccagt cagacgctgc cgccagcacc
    68701 ggtaaccacg ctttttgcgc aatgccgcct aatcccacaa cgccgatacg taattttttc
    68761 actttaatct cccagatggg ccagcaacga atcaagacgc tgtttcagtt ctgccacttc
    68821 gatttccagg gcttcgacgc gggcctgtaa atcaccatca accgcgttcg acatatccgt
    68881 taccgccggc tgatcctcta cctcaccact gaaaagatgc atgtagcggc tttcgcgttt
    68941 acccggttcg cgggccagac gcaccacaaa aggaccatct tcgcgatttg ccagttgttc
    69001 cagcgtcgac tccacttccg ccatatcgct gaattcatac attcgcgcgg cgcggctgcg
    69061 cagttcaccc ggcgtctggg caccacgcaa taacaacgtg gtgattaacg ccacttccgc
    69121 tgcgctcagt ttcagatcgc caaattctga attacaaaaa cgttgttcat atttggtgac
    69181 ccgattacca aagccactca ccgtgcgcag ataatggcgt ttgaccagat tatccagttg
    69241 ctcctgcact tcagattcac tcagattcat gaccggttca cggttcgttt tctgattaca
    69301 ggccgtgact acaccattga ctgagagcgg gtattgctcc ggcgtggtca cctgcttttc
    69361 cagcaagcaa ccaatcacgc gagcttcaag ggcagtaagt tgatatttca tgagattctc
    69421 cttagcggcc gggcgtccag tctggggtag ttaatgccgt cagtacgtga tcgcgccatt
    69481 gtccatcaat caacagatag tctttcgcat agccttcttt ttcaaaaccc agtcgcgcca
    69541 gtaaatcacc gctgcgttta ttgtgcggca tataattagc cataatgcga tgaatatgtt
    69601 gggtgcgctg catataacga atggctgcgg tcagggcttc aaacatgagt cctttgccct
    69661 gccatttttg cccaatcgaa taaccgagat agcaggcatg aaaagagcca cgaacaacat
    69721 tggaaaaatt ggcaacgcca ataatctctt tttcgtccgg gtcgaataag ccaaagtaga
    69781 aagctgaacc ttgtttatga aattcgttaa tcatccccag cctggcctgc cagcctgatg
    69841 gataacagtg gctttcgtcg cgcactggct cccagggctt gaggaaatgg cgattctctg
    69901 cgtaataatc cgcaagacgc caggcatcac gatcatgcac cagacgcacg accagtcggt
    69961 ctgtggttaa gcgcactttt ggcacgttac tgcgatagcc aaacattcta tacctactcc
    70021 ttcccgtaac gtctttaata cgctgtaagt attactatac ctgcgcaacc agaccctgtg
    70081 aaaagtccaa aatgccattt tctgcattat tcacatattt gataaaaact ctctatcaaa
    70141 gcgacgtttt gataaaaaaa tattgtcccc gaagtgatgg gaaaagtctt agcggcaatg
    70201 cagaatagaa agtgctcatc ttttatttcc cgggagggga aatgtcccgc gtatcgcagg
    70261 cgaggaacct gggtaaatat ttcctgctca tcgataatat gctggtcgtg ctggggttct
    70321 ttgttgtctt cccgctgatc tctatccgct tcgttgatca aatgggctgg gccgccgtca
    70381 tggtcggtat tgctctcggt ctacgccagt ttattcagca aggtctgggt attttcggcg
    70441 gcgcaattgc cgaccgcttt ggtgccaaac cgatgattgt taccggtatg ctgatgcgcg
    70501 ccgccggatt cgccacaatg ggtatcgccc acgaaccgtg gctattgtgg ttttcatgcc
    70561 tgctctcggg actcggcggc acgttgtttg atccgccgcg ttcggcgctg gtggtgaaac
    70621 taatccgtcc acaacaacgt tgtcgttttt tctcgctgtt gatgatgcag gacagtgccg
    70681 gtgcggtcat tggcgcattg ttggggagct ggctgttgca atacgacttt cgcctggtct
    70741 gcgccacagg ggccgttttg tttgtgctgt gtgcggcgtt caatgcgtgg ttgttaccgg
    70801 catggaaact ctccaccgta cgcacgcccg ttcgcgaagg catgacccgc gtgatgcgtg
    70861 acaagcgttt tgtcacctat gtcctgacgc tggcgggtta ctacatgctg gctgtacaag
    70921 tgatgctgat gctgccaatt atggtcaacg acgtggctgg cgcgccctct gccgttaaat
    70981 ggatgtatgc cattgaagcg tgtctgtcgt taacgttgct ctaccctatc gcccgctgga
    71041 gtgaaaagca ttttcgtctg gaacaccggt tgatggctgg gctgttgata atgtcattaa
    71101 gcatgatgcc ggtgggcatg gtcagcggtc tgcaacaact tttcaccctg atttgtctgt
    71161 tttatatcgg gtcgatcatt gccgagcctg cgcgtgaaac cttaagtgct ttgctggcgg
    71221 acgcaagagc tcgcggcagc tatatggggt ttagccgtct gggtctggcg attggcggcg
    71281 ctattggtta tatcggtggt ggctggctgt ttgacctggg caaatcggcg caccagccag
    71341 agcttccgtg gatgatgctg ggaattattg gcatcttcac tttccttgcg ctgggttggc
    71401 agtttagtca gaaacgcgca acgcgtcgtt tgctggaacg cgacgcctga tttgatactc
    71461 ccccctgact cctccatact gaaagtgaac tgacagccaa atggaggagt cacgtgaagc
    71521 tatacattta cgatcactgc ccttactgcc tcaaagcccg catgattttc ggcctgaaaa
    71581 atatccccgt cgaattacat gttctgctca acgacgacgc agaaacaccc acccggatgg
    71641 tcggtcaaaa acaggttccc attctgcaaa aagatgacag ccgctatatg ccagaaagca
    71701 tggatatcgt tcactatgtc gataaactcg acggcaaacc gttactgacc ggcaaacgtt
    71761 cccctgccat tgaagagtgg ctgcgcaagg tcaatggcta cgccaacaaa ctgctgttgc
    71821 cgcgttttgc caaatcggca tttgatgagt tttctactcc cgccgcgcgc aaatatttcg
    71881 tcgacaagaa agaggccagc gcgggtaatt ttgccgacct gctggcccac tctgacggtc
    71941 tgattaagaa tatcagcgat gatttgcgtg cgctggacaa actgatcgtc aaaccgaacg
    72001 ccgtgaatgg cgaactttcg gaagatgata ttcagctatt tccgcactgc gtaatctgac
    72061 gctggtagcc ggaatcaact ggccaagccg cgttgctgat taccgcgata atatggcgaa
    72121 acagacacaa atcaatttgt tatcatcaat ggcgatttaa tcctgacccg ccgcggcagc
    72181 ctgtattcgc gggttgccgc cgttttcagt tttttctgct ggcgagttta tcgctggtga
    72241 cacatgctgc gttgtgtcag gatattgacg cagatttcat gtcgaggact accatgaaca
    72301 agtttttatt tgccgctgca ttgatcgtca gtggcctgct cgttggctgt aatcaactca
    72361 cccaatacac catcaccgaa caagaaatta accagtcgct tgcgaaacat aataatttct
    72421 caaaagatat cggtttaccc ggcgtggctg acgcccatat tgttctgaca aacttgatca
    72481 gccaaattgg tcgcgaagag ccgaataagg ttaccctaac cggagacgcc aatctggaca
    72541 tgaactccct gttcggtagt cagaaagcga ccatgaaact gaagctgaaa gcgctgcccg
    72601 tgtttgataa agaaaaaggt gcgatcttcc tgaaagagat ggaagtggtc gatgcgacgg
    72661 tacaaccgga aaaaatgcaa acggtgatgc aaacgttgct tccctatttg aaccaggcat
    72721 tacgcaatta ctttaaccag caacctgctt acgtcctgcg cgaagatggc agtcagggcg
    72781 aagcaatggc gaaaaaactg gcgaaaggca ttgaagtgaa gccaggcgaa attgtcattc
    72841 cgtttacgga ttaatgacga gggcgcattc gcgcccttta tttttcgtgc aaaggaaaac
    72901 gtttccgctt atcctttgtg tccggcaaaa acattccttc agccggagca tagagattaa
    72961 tgactgcacc atcccaggta ttaaagatcc gccgcccaga cgactggcac cttcacctcc
    73021 gcgatggcga catgttaaaa actgtcgtgc cgtataccag cgaaatttat ggacgggcta
    73081 tcgtaatgcc caatctggct ccgcccgtga ccacagttga ggctgccgtg gcgtatcgcc
    73141 aacgtattct tgacgccgta cctgccgggc acgatttcac cccattgata acctgttatt
    73201 taacagattc gctggatcct aatgagctgg agcgcggatt taatgaaggc gtgttcaccg
    73261 ctgcaaaact ttacccggca aacgcaacca ctaactccag ccacggcgtg acgtcaattg
    73321 acgcaatcat gccggtactt gagcgcatgg aaaaaatcgg tatgccgcta ctggtgcatg
    73381 gtgaagtgac acatgcagat atcgacattt ttgatcgtga agcgcgcttt atagaaagcg
    73441 tgatggaacc tctgcgccag cgcctgactg cgctgaaagt cgtttttgag cacatcacca
    73501 ccaaagatgc tgccgactat gtccgtgacg gaaatgaacg gctggctgcc accatcactc
    73561 cgcagcatct gatgtttaac cgcaaccata tgctggttgg tggcgtgcgt ccgcacctgt
    73621 attgtttacc catcctcaaa cgcaatattc accaacaggc attgcgtgaa ctggtcgcca
    73681 gcggttttaa tcgagtattc ctcggtacgg attctgcgcc acatgcacgt catcgcaaag
    73741 agagcagttg cggctgcgcg ggctgcttca acgccccaac cgcgctgggc agttacgcta
    73801 ccgtctttga agagatgaat gctctgcagc actttgaagc attctgttct gtaaacggcc
    73861 cgcagttcta tgggttgccg gtcaacgaca cattcatcga actggtacgt gaagagcaac
    73921 aggttgctga aagcatcgca ctgactgatg acaccctggt gccattcctc gccggggaaa
    73981 cggtacgctg gtccgttaaa caataaaaat cgtagcttcc tgttgtcatt aggttattta
    74041 acctgtataa ataaccagta tattcaacag ggggctatta tgcgaattga agtcaccata
    74101 gcgaaaactt ctccattgcc agctggggct attgacgccc tggctggcga actttcccgc
    74161 cgtattcagt atgcgtttcc tgataatgaa ggccacgtat cggtacgtta tgccgcagcg
    74221 aataatttat cggttattgg cgcaacaaaa gaagataaac agcgcattag cgaaattctc
    74281 caggaaacgt gggaaagcgc cgatgactgg tttgtcagcg aataatatgc agtgattttt
    74341 gtttttgccg ggtcgccccg gcttttttat tttacttacg aattttgagt acaaaatttc
    74401 tacacttcct caaaataatt gcacaatatg ctaaaaaaat atgaatgaca tgctgtttta
    74461 ttgttcatat aaatcttaaa agcagaaatt tatctgttcc cccctttaaa aattcggtga
    74521 atacccttac ttattggtat actgaagttg cttcagataa aaacgcattg aacctcgaat
    74581 aacgttgtct agtaacacga attagggggc catgatggaa aaaaataatg aagtcattca
    74641 gactcatccg ctcgtagggt gggacatcag caccgttgat agctatgatg cgctgatgtt
    74701 gcgtttgcac taccagaccc caaataagtc cgagcaggaa gggactgaag ttggtcaaac
    74761 gctctggtta accactgatg ttgccagaca atttatttcg atattagaag caggaatcgc
    74821 caaaattgaa tccggtgatt tccaggtaaa cgagtatcgg cgtcattaac atgacatgac
    74881 catccaccca aaaggcacct caccggtgcc tttaccattt ctgggttgta tatctgcatt
    74941 cagttaatta ctctgcttaa accatgagag cagagagaaa aaaatgaaat acgatctcat
    75001 cattattggc agcggttccg taggcgctgc cgccgggtat tatgcaaccc gcgccggttt
    75061 aaacgtgcta atgaccgacg cccatatgcc accgcatcaa cacggcagcc accacggcga
    75121 tacgcgatta attcgccatg cttatggtga aggcgaaaag tatgtcccgc tggtcctccg
    75181 cgcgcaaacg ctgtgggatg aactttcccg ccacaacgaa gatgatccca tttttgtacg
    75241 ctctggtgtc attaacctcg gcccggctga ctccgcattt ctcgccaacg tcgcccacag
    75301 cgccgaacag tggcaactca acgttgaaaa gctcgatgcg caagggatta tggcccgctg
    75361 gccagaaata cgcgtcccgg acaactacat cggcttattt gagactgatt ccggtttttt
    75421 gcgcagcgaa ctggcgatta aaacctggat ccaactggcg aaggaagcgg gctgtgcgca
    75481 actgttcaac tgcccggtca ccgcaattcg tcatgacgat gatggcgtaa ctattgaaac
    75541 ggttgacggt gagtatcagg cgaaaaaagc gattgtctgc gcgggaacat gggtaaaaga
    75601 cctgctcccg gagcagcctg tccagcccgt acgtaaagta tttgcctggt atcaggccga
    75661 tggccgctat agcgtgaaga ataaattccc ggcgtttacc ggtgaactgc ccaatggcga
    75721 tcaatattat ggttttccgg cagaaaacga cgcgttgaag attggcaaac ataacggagg
    75781 ccaggttatc cattcagcgg atgaacgtgt tccgtttgcg gaagtggtca gcgatggttc
    75841 ggaagccttc ccgttcttgc gcaatgtatt gccgggtatc ggttgctgcc tgtacggcgc
    75901 tgcctgcacc tatgataatt cgcctgacga agattttatt atcgataccc tacccggcca
    75961 cgataataca ctgctcatta ccggcctgag tgggcacggt tttaaatttg cgtcagtttt
    76021 aggggaaata gctgccgatt ttgcgcaaga caaaaaaagc gattttgatt tgacgccatt
    76081 caggctttcc cgcttccaat aatcatcatt acggcctcca gaatgaggcc gtttttacag
    76141 gagtatttaa tgcgtcggct gttgcactat ctca
//
LOCUS       DAZPLI010000002        74504 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZPLI010000002 DAZPLI010000000
VERSION     DAZPLI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 74504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74504
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.002"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHUU3_000077"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHUU3_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR2080268.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="ACHUU3_000078"
     tRNA            1230..1305
                     /locus_tag="ACHUU3_000078"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="ACHUU3_000079"
     CDS             complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="ACHUU3_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR2080269.1"
                     /translation="MMRRVNIFCSFALLFASHNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="ACHUU3_000080"
     CDS             complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="ACHUU3_000080"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR2080270.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="ACHUU3_000081"
     CDS             complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="ACHUU3_000081"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR2080271.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="ACHUU3_000082"
     CDS             complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="ACHUU3_000082"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR2080272.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4868..5305)
                     /locus_tag="ACHUU3_000083"
     CDS             complement(4868..5305)
                     /locus_tag="ACHUU3_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFR2080273.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5523..5698)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5787..6463)
                     /locus_tag="ACHUU3_000084"
                     /pseudo
     CDS             complement(5787..6463)
                     /locus_tag="ACHUU3_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="ACHUU3_000085"
     CDS             complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="ACHUU3_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFR2080274.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            7987..8271
                     /gene="pduA"
                     /locus_tag="ACHUU3_000086"
     CDS             7987..8271
                     /gene="pduA"
                     /locus_tag="ACHUU3_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFR2080275.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            8268..9080
                     /gene="pduB"
                     /locus_tag="ACHUU3_000087"
                     /pseudo
     CDS             8268..9080
                     /gene="pduB"
                     /locus_tag="ACHUU3_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
     gene            9099..10763
                     /gene="pduC"
                     /locus_tag="ACHUU3_000088"
     CDS             9099..10763
                     /gene="pduC"
                     /locus_tag="ACHUU3_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFR2080276.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            10773..11441
                     /locus_tag="ACHUU3_000089"
     CDS             10773..11441
                     /locus_tag="ACHUU3_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFR2080277.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            11456..11974
                     /gene="pduE"
                     /locus_tag="ACHUU3_000090"
     CDS             11456..11974
                     /gene="pduE"
                     /locus_tag="ACHUU3_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFR2080278.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            11984..13816
                     /locus_tag="ACHUU3_000091"
     CDS             11984..13816
                     /locus_tag="ACHUU3_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFR2080279.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            13806..14156
                     /locus_tag="ACHUU3_000092"
     CDS             13806..14156
                     /locus_tag="ACHUU3_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFR2080280.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            14176..14451
                     /gene="pduJ"
                     /locus_tag="ACHUU3_000093"
     CDS             14176..14451
                     /gene="pduJ"
                     /locus_tag="ACHUU3_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFR2080281.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            14475..14897
                     /locus_tag="ACHUU3_000094"
     CDS             14475..14897
                     /locus_tag="ACHUU3_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFR2080282.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            14900..15532
                     /locus_tag="ACHUU3_000095"
     CDS             14900..15532
                     /locus_tag="ACHUU3_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFR2080283.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            15529..16020
                     /gene="pduM"
                     /locus_tag="ACHUU3_000096"
     CDS             15529..16020
                     /gene="pduM"
                     /locus_tag="ACHUU3_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFR2080284.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            16024..16299
                     /gene="pduN"
                     /locus_tag="ACHUU3_000097"
     CDS             16024..16299
                     /gene="pduN"
                     /locus_tag="ACHUU3_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HFR2080285.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            16311..17318
                     /gene="pduO"
                     /locus_tag="ACHUU3_000098"
     CDS             16311..17318
                     /gene="pduO"
                     /locus_tag="ACHUU3_000098"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFR2080286.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            17315..18700
                     /gene="pduP"
                     /locus_tag="ACHUU3_000099"
                     /pseudo
     CDS             17315..18700
                     /gene="pduP"
                     /locus_tag="ACHUU3_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            18711..19823
                     /locus_tag="ACHUU3_000100"
     CDS             18711..19823
                     /locus_tag="ACHUU3_000100"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010835540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFR2080287.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            19820..21163
                     /locus_tag="ACHUU3_000101"
     CDS             19820..21163
                     /locus_tag="ACHUU3_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR2080288.1"
                     /translation="MNVGLISCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARADTFL
                     VNAAECEPMLKVDQQLMAQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVSVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTVNGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGSLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLAVAKTVCEQCRLCTDLCPRHLIG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLTPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            21166..21720
                     /gene="pduT"
                     /locus_tag="ACHUU3_000102"
     CDS             21166..21720
                     /gene="pduT"
                     /locus_tag="ACHUU3_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFR2080289.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            21720..22070
                     /gene="pduU"
                     /locus_tag="ACHUU3_000103"
     CDS             21720..22070
                     /gene="pduU"
                     /locus_tag="ACHUU3_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFR2080290.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            22075..22518
                     /locus_tag="ACHUU3_000104"
     CDS             22075..22518
                     /locus_tag="ACHUU3_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR2080291.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(22619..22948)
                     /locus_tag="ACHUU3_000105"
     CDS             complement(22619..22948)
                     /locus_tag="ACHUU3_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR2080292.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(23120..24178)
                     /locus_tag="ACHUU3_000106"
     CDS             complement(23120..24178)
                     /locus_tag="ACHUU3_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR2080293.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(24376..24849)
                     /gene="sbmC"
                     /locus_tag="ACHUU3_000107"
     CDS             complement(24376..24849)
                     /gene="sbmC"
                     /locus_tag="ACHUU3_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFR2080294.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(24968..26134)
                     /gene="dacD"
                     /locus_tag="ACHUU3_000108"
     CDS             complement(24968..26134)
                     /gene="dacD"
                     /locus_tag="ACHUU3_000108"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFR2080295.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            26343..27770
                     /gene="sbcB"
                     /locus_tag="ACHUU3_000109"
     CDS             26343..27770
                     /gene="sbcB"
                     /locus_tag="ACHUU3_000109"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR2080296.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(27813..28040)
                     /gene="tsuB"
                     /locus_tag="ACHUU3_000110"
     CDS             complement(27813..28040)
                     /gene="tsuB"
                     /locus_tag="ACHUU3_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR2080297.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(28054..29112)
                     /gene="tsuA"
                     /locus_tag="ACHUU3_000111"
     CDS             complement(28054..29112)
                     /gene="tsuA"
                     /locus_tag="ACHUU3_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR2080298.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(29290..30648)
                     /gene="plaP"
                     /locus_tag="ACHUU3_000112"
     CDS             complement(29290..30648)
                     /gene="plaP"
                     /locus_tag="ACHUU3_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR2080299.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(30638..30700)
                     /gene="yoeI"
                     /locus_tag="ACHUU3_000113"
     CDS             complement(30638..30700)
                     /gene="yoeI"
                     /locus_tag="ACHUU3_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR2080300.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(30915..31844)
                     /locus_tag="ACHUU3_000114"
     CDS             complement(30915..31844)
                     /locus_tag="ACHUU3_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR2080301.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(31890..32714)
                     /locus_tag="ACHUU3_000115"
     CDS             complement(31890..32714)
                     /locus_tag="ACHUU3_000115"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR2080302.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(32797..33051)
                     /gene="yoeB"
                     /locus_tag="ACHUU3_000116"
     CDS             complement(32797..33051)
                     /gene="yoeB"
                     /locus_tag="ACHUU3_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR2080303.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(33048..33299)
                     /gene="yefM"
                     /locus_tag="ACHUU3_000117"
     CDS             complement(33048..33299)
                     /gene="yefM"
                     /locus_tag="ACHUU3_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR2080304.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            33582..33632
                     /gene="hisL"
                     /locus_tag="ACHUU3_000118"
     CDS             33582..33632
                     /gene="hisL"
                     /locus_tag="ACHUU3_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFR2080305.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    33608..33731
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            33778..34677
                     /gene="hisG"
                     /locus_tag="ACHUU3_000119"
     CDS             33778..34677
                     /gene="hisG"
                     /locus_tag="ACHUU3_000119"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR2080306.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            34683..35987
                     /gene="hisD"
                     /locus_tag="ACHUU3_000120"
     CDS             34683..35987
                     /gene="hisD"
                     /locus_tag="ACHUU3_000120"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR2080307.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            35984..37054
                     /gene="hisC"
                     /locus_tag="ACHUU3_000121"
     CDS             35984..37054
                     /gene="hisC"
                     /locus_tag="ACHUU3_000121"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR2080308.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            37054..38121
                     /gene="hisB"
                     /locus_tag="ACHUU3_000122"
     CDS             37054..38121
                     /gene="hisB"
                     /locus_tag="ACHUU3_000122"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR2080309.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            38121..38711
                     /gene="hisH"
                     /locus_tag="ACHUU3_000123"
     CDS             38121..38711
                     /gene="hisH"
                     /locus_tag="ACHUU3_000123"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR2080310.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            38711..39448
                     /gene="hisA"
                     /locus_tag="ACHUU3_000124"
     CDS             38711..39448
                     /gene="hisA"
                     /locus_tag="ACHUU3_000124"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR2080311.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            39430..40206
                     /gene="hisF"
                     /locus_tag="ACHUU3_000125"
     CDS             39430..40206
                     /gene="hisF"
                     /locus_tag="ACHUU3_000125"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR2080312.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            40200..40811
                     /gene="hisIE"
                     /locus_tag="ACHUU3_000126"
     CDS             40200..40811
                     /gene="hisIE"
                     /locus_tag="ACHUU3_000126"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR2080313.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(40908..41885)
                     /gene="wzzB"
                     /locus_tag="ACHUU3_000127"
     CDS             complement(40908..41885)
                     /gene="wzzB"
                     /locus_tag="ACHUU3_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR2080314.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(42031..43197)
                     /gene="ugd"
                     /locus_tag="ACHUU3_000128"
     CDS             complement(42031..43197)
                     /gene="ugd"
                     /locus_tag="ACHUU3_000128"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFR2080315.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILSSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(43447..44853)
                     /gene="gndA"
                     /locus_tag="ACHUU3_000129"
     CDS             complement(43447..44853)
                     /gene="gndA"
                     /locus_tag="ACHUU3_000129"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR2080316.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(45017..46387)
                     /gene="cpsG"
                     /locus_tag="ACHUU3_000130"
                     /gene_synonym="manB"
     CDS             complement(45017..46387)
                     /gene="cpsG"
                     /locus_tag="ACHUU3_000130"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR2080317.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(46492..47928)
                     /gene="cpsB"
                     /locus_tag="ACHUU3_000131"
     CDS             complement(46492..47928)
                     /gene="cpsB"
                     /locus_tag="ACHUU3_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR2080318.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(47931..49154)
                     /gene="wcaI"
                     /locus_tag="ACHUU3_000132"
     CDS             complement(47931..49154)
                     /gene="wcaI"
                     /locus_tag="ACHUU3_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR2080319.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(49151..49630)
                     /gene="gmm"
                     /locus_tag="ACHUU3_000133"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(49151..49630)
                     /gene="gmm"
                     /locus_tag="ACHUU3_000133"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR2080320.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(49633..50598)
                     /gene="fcl"
                     /locus_tag="ACHUU3_000134"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(49633..50598)
                     /gene="fcl"
                     /locus_tag="ACHUU3_000134"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR2080321.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(50601..51722)
                     /gene="gmd"
                     /locus_tag="ACHUU3_000135"
     CDS             complement(50601..51722)
                     /gene="gmd"
                     /locus_tag="ACHUU3_000135"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR2080322.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(51748..52296)
                     /gene="wcaF"
                     /locus_tag="ACHUU3_000136"
     CDS             complement(51748..52296)
                     /gene="wcaF"
                     /locus_tag="ACHUU3_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR2080323.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(52312..53058)
                     /gene="wcaE"
                     /locus_tag="ACHUU3_000137"
     CDS             complement(52312..53058)
                     /gene="wcaE"
                     /locus_tag="ACHUU3_000137"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR2080324.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(53069..54286)
                     /gene="wcaD"
                     /locus_tag="ACHUU3_000138"
     CDS             complement(53069..54286)
                     /gene="wcaD"
                     /locus_tag="ACHUU3_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFR2080325.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(54261..55478)
                     /gene="wcaC"
                     /locus_tag="ACHUU3_000139"
     CDS             complement(54261..55478)
                     /gene="wcaC"
                     /locus_tag="ACHUU3_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR2080326.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(55475..55963)
                     /gene="wcaB"
                     /locus_tag="ACHUU3_000140"
     CDS             complement(55475..55963)
                     /gene="wcaB"
                     /locus_tag="ACHUU3_000140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR2080327.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(55966..56805)
                     /gene="wcaA"
                     /locus_tag="ACHUU3_000141"
     CDS             complement(55966..56805)
                     /gene="wcaA"
                     /locus_tag="ACHUU3_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFR2080328.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(56898..59060)
                     /gene="wzc"
                     /locus_tag="ACHUU3_000142"
     CDS             complement(56898..59060)
                     /gene="wzc"
                     /locus_tag="ACHUU3_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR2080329.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(59063..59506)
                     /gene="wzb"
                     /locus_tag="ACHUU3_000143"
     CDS             complement(59063..59506)
                     /gene="wzb"
                     /locus_tag="ACHUU3_000143"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR2080330.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(59512..60651)
                     /gene="wza"
                     /locus_tag="ACHUU3_000144"
     CDS             complement(59512..60651)
                     /gene="wza"
                     /locus_tag="ACHUU3_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR2080331.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            60960..61109
                     /locus_tag="ACHUU3_000145"
     CDS             60960..61109
                     /locus_tag="ACHUU3_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2080332.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            61310..62893
                     /locus_tag="ACHUU3_000146"
     CDS             61310..62893
                     /locus_tag="ACHUU3_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR2080333.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(63167..65020)
                     /gene="asmA"
                     /locus_tag="ACHUU3_000147"
     CDS             complement(63167..65020)
                     /gene="asmA"
                     /locus_tag="ACHUU3_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR2080334.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(65042..65623)
                     /gene="dcd"
                     /locus_tag="ACHUU3_000148"
     CDS             complement(65042..65623)
                     /gene="dcd"
                     /locus_tag="ACHUU3_000148"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR2080335.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(65715..66356)
                     /gene="udk"
                     /locus_tag="ACHUU3_000149"
     CDS             complement(65715..66356)
                     /gene="udk"
                     /locus_tag="ACHUU3_000149"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR2080336.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            66674..69991
                     /locus_tag="ACHUU3_000150"
     CDS             66674..69991
                     /locus_tag="ACHUU3_000150"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR2080337.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGILVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(70101..70949)
                     /gene="alkA"
                     /locus_tag="ACHUU3_000151"
     CDS             complement(70101..70949)
                     /gene="alkA"
                     /locus_tag="ACHUU3_000151"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFR2080338.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            71043..72395
                     /gene="yegD"
                     /locus_tag="ACHUU3_000152"
     CDS             71043..72395
                     /gene="yegD"
                     /locus_tag="ACHUU3_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR2080339.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(72408..74348)
                     /gene="yegI"
                     /locus_tag="ACHUU3_000153"
     CDS             complement(72408..74348)
                     /gene="yegI"
                     /locus_tag="ACHUU3_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFR2080340.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(74345..>74504)
                     /locus_tag="ACHUU3_000154"
     CDS             complement(74345..>74504)
                     /locus_tag="ACHUU3_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HFR2080341.1"
                     /translation="VPFFTPFFNGLASATQEQLDLLPELLKQFLSSPAVNERTDDDKT
                     LALALWLP"
BASE COUNT        18570 a        19627 c        18429 g        17878 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaacatc agcgcccggg ttcgcttcca
     2101 gcatgttact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgggtattgt
     2161 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     2221 gataagttac cgccaggcta ttatggctgg caaaaagcag agcaaatgag caaaaaatat
     2281 ttacacgacg catcatgtcc ctttcctaag tcgcgaaagc tatccgttaa gtatagcttt
     2341 tatcagactt ttcgttttta actgttcaaa tcagaagtcg tattccccgg tagaacaata
     2401 ttactggcag caagttcgcc catgttgtta tatatcgcac aggcagcttc gatgatgggc
     2461 atcgccagag ctgcgccact cccctcacct aaacgcatct ccatattgag ataaggctcc
     2521 agccccaaat gcgacagcgc aatacgcgcc cctttttctg ccgacaggtg agaaggaatg
     2581 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcggcagc ataagaaaga
     2641 aatccatcca gcaacacggg taaaccgcag gaagcagcgc ctaacatcac gccagccatt
     2701 ccgaccaaat caaatccacc cacttttgcc aggacatcaa cgccatcctg cggatttggt
     2761 tgattcaacg taatcgcccg gcgcacaaca tcaattttat tagccagttt atctgtcggc
     2821 aggtttgcgc caatcccaac cacttcttca ggagcccggc cagtgattgt gctgactatt
     2881 gccgctgccg gtgtcgtgtt tgccatcccc aggtcaccta caccaaacag cgtaacaccg
     2941 ttttttgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc tgccagatgg
     3001 cgactcattg ccggagctga agcaatattg ccactacctc gagcaacacg catgttgata
     3061 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtagac gttagcgccc
     3121 gcttgtgctg ccagcacaca cacgccggtt gttccgcggg tcatattttc agcctgaatg
     3181 gctgtcactt cttttgggga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     3241 atgaccagta ccgctttttt gcccacatgc ggaacgccat tcaaccccgg cattcctgcc
     3301 agttgtatgg caagcgcctc cagccttccc aggctaccaa caggtttgag taacccgtcg
     3361 atatgccgtt gtgcacgcga catagcggca ggatcaatag cagggatcgt attcagtaaa
     3421 tcggcaagtg tttgcatgtc acgtctcttt atagcagtgc cagcaggaag accagttcac
     3481 caagctctat agcagctccc agcgtatcgc cggtttgccc gcccagcgta cgtttgagaa
     3541 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     3601 ccggcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     3661 catcaatttt gccaatgaac acattgccaa gtccttcttc ccgggcataa cgatgacgat
     3721 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
     3781 tcggttcgcc acgcagagcc aactcgctta ataccagaat ctttgccagt acaacaaaaa
     3841 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
     3901 cgcggctacg tgcagaaaat acgccatcac aggtatcggc gaggccgtcc aggtggaatc
     3961 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt acgccacacc
     4021 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaattaaag
     4081 gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac cagcgacgcg
     4141 gtacaggcag acgcgtaata aaagagagca tcgcccaaaa taatttactc atttgatttt
     4201 tactccaata cccgaaacca ccaaccatac ttcatttgcc gcagcggcca gctgctgatt
     4261 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
     4321 tcccacttcg ttagtcacta atacaacctt tgcgggacaa cgttggcagg cagcaatcaa
     4381 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
     4441 tttatcgccg ccataatcaa acaacagatt agtcaccatg gtggtaacgc attccagcaa
     4501 tacagcctca tgcgggttaa tgtctgcatg aattaattca tcaagatgtt gccagcgctc
     4561 cactgtgtgc cagtgcgccg ggcggccttg ccgatgatgt tctatccgtg cagccatctc
     4621 atcatcaagg atttgtgagg tggcgatata cagaacctgc gaagagtccc caataagcgc
     4681 ctctgcatgg cgactcttcc cgctccgtgc gccccccgtc accaaaatca tcataccgac
     4741 tcctgattca attgtatgat ggtattcatt gaacagaaca atggatcccc caccgttgct
     4801 gaccgcaaaa catttacgcg attaatactg cgcgtaatat aattaaaaat ccagcattct
     4861 caatccatta cgaaagaaga tggcattgcg gaatagccag acttcctcgt gttaacgtcc
     4921 gacaaaatgc gtcattgaca gcggtttctc tggaatgaca gatttcattc agataatgga
     4981 ttaatgctac agccccacca gccggaccag tcagcaaacg ggcattgtgt tctgcaatca
     5041 aaattaccgt cgttccctca gaatgagcga gccattctgg tacggatatc tcgctttcag
     5101 ctgctatcaa tagcagcact gaataatcgc tatacgcatc tgataacgat tgcacaggcc
     5161 caccatcaag aggctggaaa tcttgctgcc agtcctcacc aacaaaacag gttattggca
     5221 atatttcagc cagatgtttc gccagatcca catctccaga taagaggcaa aaaagagcta
     5281 tcgattcggg ttttatcgtt ttcatgcctc tcgccctcca cttaatttcc tgtaatggcg
     5341 gtctggttga taagccatga aacaatattg ccacaccttg aatgcagccc tggtgcctgt
     5401 cggcactgcg atgaaagaac taaatgatgc cgccataccc tgatcctttc agggtggtag
     5461 gggtataaag acgcgccagt actctaccgg acgcttccgg gcaacccact tcccaccgaa
     5521 gttgttggta ttgactgaca gactggtctt ctggcttagc gtcatcctca cccgaccttc
     5581 ccaatctcgc gatcagtggc attttcgggt ttgtcagcat tacagcagcg ggggctgcga
     5641 gggattttca ccctcttccc aggcacaaat gtgccacacc tgtcagcgta tgttatgagc
     5701 tgatttcact tttcccataa cacgtaaata acgttaatta ttcattccag cgtttattta
     5761 aaccttccct ttttttagca tttattctat accgtttcca tcgccgcatt ttcactcact
     5821 tgctgtcgat acacctgcgg cgtaacctga taagtctgac ggaaaacttt gcaaaaataa
     5881 ctgtattgtc gtggggatta ggcgcacgag tctggcgcac cgaatctgac cgagatttgt
     5941 tctctttaat aaccacaaaa ttgagttgtt ttttgaggca attttccaca atcaacttca
     6001 acaaatcagc agaggccata actcttgagt aatccatctc aggaactttg cggaattcat
     6061 taagtaattc gggatctgcc tgccagcaat catcgacatt cagaatatcg accaaatcga
     6121 catcatcatt acgcagacga acttgcccgc acaatacaaa accgactaaa tgtccggcga
     6181 ttaccagtgg aatagagaaa tcggttaatc cggcatgaca gcgataaata caaggctgat
     6241 cggatttaga ggcttccagg cccccacaac gatcgctcat gcgacatcga gtgcttagct
     6301 gagggtgttg ccgcatcatc tgacaaaatg gagtgaagtt aaacagctcg gaaatttcat
     6361 cgccgtgaat attgaccacg acaaccgcaa ggctggtcgc ctgcgcaaaa tcctgcgcga
     6421 ttttattaat gagttctgaa ttaagtgtcg ccgcagaaat catgataaaa cctcccagtt
     6481 aatttattgt tatagcaaaa caattgtttt acatttaatc ttcacatcat ccctgatgat
     6541 ttactgacga gaagcacaaa ggacaataac aaaagcctcc gtgaagcggg agacttttta
     6601 ataagttcgg aaagcagatc acaaacagta aaaaaataaa tgtttataac tgccagagta
     6661 tttcgttatt cgctaattcc tttaggtgta acactattga caggtaaatt ctttgcaatg
     6721 agatagcgat aaatcgccgc accaacacat gccccaatga tcggtgcaat aatgggtaca
     6781 atgaaataag gaatattgcg tccaccagtc atcgcaattt ctccccaccc cgcaagccag
     6841 gtgaaaatct taggtccaaa atcacgcgct ggattcatgg caaaaccggt taatggtccg
     6901 gtagaagcac caataacagc aaccagaata ccaataagta aaggtgcaag cggtcctttg
     6961 ggtacgccat taccgtcatc ggtcaacgcc ataatcaacc ccattaacat ggacgttatg
     7021 accacttcga ccaacgcagc ttgccataca ttaatcgccg ctgccggata agtactgaag
     7081 atactggcga gttgtaaact ttctgcgcta ccacgcacca tattgtgagc agattcaaaa
     7141 tcggtaaaca gactactgta tagagaatat gccagaactg caccaccaaa ggcacccgct
     7201 aattgcgcca gacaaaatgg cagcacttta cgggctggga aagaggcaaa cagccacaga
     7261 gcaatggtaa ctgccgggtt gagatgagcg ccggaaatac ctgccgtaag gtaaacagca
     7321 agcgaaatcc ctaaccccca aatgatacaa atttcccaca gtcccagact tgccccggca
     7381 actttcaggg cgcacaaaca accaatgcca aaaaagagga acagtccggt gcccaaaaac
     7441 tcggcaacgc attgcgcctt gagtgaatca ttcatagtga cacctttttt acggagtttt
     7501 tctttatcag ccaactatgc gctgactgtt gtatataccg agcacaacta taaaaaattg
     7561 ctctgcagaa aatattgcca acatgcatga cagactcgtg taaaaatcgt gctaacataa
     7621 caaattattg tcattaccga atcatgcaac acagcgttag tcagtgttga aagtaataat
     7681 tcccaccaaa cttttacaat aaaaaattgc catcatgaca taataaaatt gaaacaaata
     7741 tcacagttcc ttcttcgaac taaactcaac caaatattac tcgcgattaa actcactagc
     7801 caacgcaatt aattcacatt gaaatatcca caaaacaacc aataaattaa caacatatag
     7861 ttcataaaag caattttata atatttgttc tctattaatt tctctcatta gccgattaag
     7921 tcagccattc tttttcctcg ctttttttta tagtcatatc gtcaaaaaaa tccaggcgag
     7981 gtctttatgc aacaagaagc gttaggaatg gtagaaacca aaggcttaac tgccgccata
     8041 gaggccgcag acgccatggt gaagtcggca aatgttttgc tggtcggcta cgaaaaaata
     8101 ggttccgggc tggtgaccgt catcgttcgt ggtgatgtcg gggcggtaaa agcggcaaca
     8161 gacgcgggtg ccgcagctgc gcataacgtc ggcgaagtaa aagccgtaca cgttattccc
     8221 cgcccacata ccgatgtcga aaaaatctta ccaaagggaa ttaacccatg agcagcaatg
     8281 aactggtcga acagatcatg gcgcaggtca tcgctcgcgt agcgacgcca gagcatgagg
     8341 ttctcccgaa aaccactcat caaatacgat agacggctat ggcagaaaaa agctgcacgc
     8401 taacagaatt tgttggcacc gctattggcg acactgtagg tctggtcatt gcgaacgttg
     8461 atagcgccct gttggacgcg atgaaacttg aaaaacgcta tcgttccatt ggcattctgg
     8521 gtgcgcgtat tggcgcaggg ccacacatta tggccgctga tgaagcggta aaagccacca
     8581 acactgaagt tattagcatt gaacttcctc gcgatactaa aggcggtgcc ggacacggct
     8641 cactgatcct cttaggtggc aacgatgttt ccgacgtgaa acgcggtatt gaagtcgccc
     8701 tgaaagaact cgatcggacc tttggcgatg tctatgccaa cgaagccgga catcttgaac
     8761 tgcaatatac cgcccgcgcc agctatgcac tggaaaaagc gtttggtgca ccactgggac
     8821 gggcttgcgg cgttattgtc ggcgctccgg ctgctgtggg tgttctgatg gccgacaccg
     8881 cgctgaagtc cgccaatgtg gatgttgtgg catacagctc cccagctcaa ggcaccagct
     8941 acagcaatga agtcattctg attatctcgg gtgactccgg cgcagtccgc caggcggtca
     9001 tctccgcacg cgaaatcggt aaaaccgtgc tttcgactct cggcgataca ccgaaaaacg
     9061 atcacccttc ctacatctga attctctgag gcagatttat gagatcgaaa agatttgaag
     9121 cactggcaaa acgcccggtc aaccaggacg gtttcgttaa agagtggatt gaagaaggct
     9181 ttattgcgat ggaaagcccg aacgatccta aaccgtcaat caaaattgtt aacggcgtcg
     9241 ttaccgaact ggatggaaaa cccgccagcc agtttgacct gatcgaccac tttatcgccc
     9301 gctacggcat caatctcgcg cgcgccgaag aagtcatcgc gatggattcg gtaaaacttg
     9361 ccaatatgtt gtgcgatcca aatgtcaaac gtagcgatat cgttcccctt accacagcaa
     9421 tgacgcctgc aaaaatcgtc gaagtggttt cgcagatgaa cgtggtagag atgatgatgg
     9481 cgatgcagaa aatgcgcgcc cgtcgaacac catcacaaca ggctcacgtc accaacgtca
     9541 aagataaccc agtacaaatt gccgccgacg cagctgaagg cgcatggcgc gggtttgatg
     9601 aacaggaaac caccgttgcc gttgctcgct atgcaccgtt caacgccatc gccctgctgg
     9661 tcggctcaca ggtgggacgt ccgggcgtct tgacccagtg ttcactggaa gaagccaccg
     9721 aactgaaact cggtatgtta ggccacacct gttacgccga aacaatttct gtctatggta
     9781 cagaacctgt tttcaccgat ggtgatgata ccccgtggtc aaaagggttt ctcgcgtcat
     9841 cctatgcttc tcgtggctta aaaatgcgct ttacttccgg ctccggctct gaagtacaga
     9901 tgggttacgc ggaaggcaaa tcaatgcttt acctcgaggc tcgttgcatt tacatcacca
     9961 aagccgcagg cgttcagggt ctgcagaatg gttcggtaag ctgcatcggt gttccatcag
    10021 ctgtgccatc aggcatccgc gcagtattag cggaaaactt aatttgctca tcgctggatc
    10081 tggaatgcgc ctccagtaat gaccagacct ttacccactc ggatatgcgc cgtaccgcac
    10141 gtttcctgat gcaatttctg ccaggcaccg actttatctc ttccggtttt tccgctgtgc
    10201 cgaactacga caacatgttt gctggttcaa acgaagatgc tgaagacttc gatgattaca
    10261 acgtcattca gcgtgacctg aaggttgatg gtgggctgcg accagtacgc gaagaagatg
    10321 ttatcgctat ccgcaataaa gccgctcgcg ctttacaggc tgtctttgcc ggtatgggac
    10381 taccgcctat taccgatgaa gaagtcgaag cggcaaccta tgcccacggt tcaaaagata
    10441 tgcctgagcg caatattgtg gaagacatca agttcgccca ggagattatc aataaaaacc
    10501 ggaacagcct ggaagtggtg aaagccttgg cgcaaggcgg ttttaccgat gtcgcccagg
    10561 acatgctcaa catgcaaaaa gcaaaactga ccggcgatta tctccatacc tcagccatca
    10621 tcgtcgatga cggacaagtg ctctctgcgg tcaatgacgt caatgattac gccggaccgg
    10681 ctacaggtta ccgcctgcaa ggggaacgct gggaagagat caaaaatatt cccggtgcac
    10741 ttgatcccaa cgaaattgac taagggtgag aaatggaaat taacgaaaaa ttactgcgtc
    10801 agattatcga agacgtgctg gcagaaatgc aacccagcga caagtccgtt tcatttcgcg
    10861 cacctgtctc tgcaactgtc ccttccgcgc cagataccgg aaattttctg accgaaattg
    10921 gcgaagccca acaaggcact cagcaagatg aagtcattat tgctgtaggc ccggcgttcg
    10981 gcctggcgca aaccgtcaat atcatcggta ttccgcataa aaacatcttg cgcgaagtga
    11041 ttgccggtat cgaagaagaa ggcattaaag cccgtgtaat tcgctgcttt aaatcgtccg
    11101 atgtcgcatt cgtagcagtc gaaggcgatc gtcttagtgg ttcaggtatt gctatcggta
    11161 ttcagtcgaa aggcacaacg gttatccacc agcagggact tcctcccttg tctaacctgg
    11221 aacttttccc acaagcgcct ttgttgacgc tggatactta tcgtcagatt ggcaaaaacg
    11281 ccgcgcgtta tgcgaagcgc gagtctccac aaccggtgcc gaccctcaac gatcaaatgg
    11341 cgcggccgaa ataccaagcc aaatctgcca ttttgcatat taaagagacg aagtacgtgg
    11401 tcactggcaa aaaaccgcaa gaactgcgcg tgacgtttta acaaaggaaa ccttcatgaa
    11461 taccgatgca attgagtcga tggtcaggga cgtgttgaac cgcatgaaca gtttacagga
    11521 tgcagctcct gtttctgcgg tccccaacgc atcaatactg agcgccaaag tgacggatta
    11581 cccgctggca aataaacatc cggaatgggt taaaacagcg accaataaaa cgctcgacga
    11641 tttcactctg gaaaatgtgc taagcgataa cgtcaccgcc ctggatatgc gcattacccc
    11701 tgaaacattg cgtattcagg ctgcgattgc cagagatgct ggtcgcgatc gtctggcgat
    11761 gaattttgaa cgcgccgctg agcttacctc agtaccagat gatcgcatcc tcgaaatcta
    11821 caatgcgtta cgcccttatc gttcaactaa gcaagaactt attgccattg ctgacgatct
    11881 tgaacagcgt tatcaggcca aaatttgtgc tgcatttgtc cgtgaagctg cggaacttta
    11941 tgtcgagcgc aaaaaactga aaggcgacga ttaaggacga actatgcgat atatagcagg
    12001 cattgatatc ggcaactcat caacagaggt tgcgctggca acgctcagtg caactggaga
    12061 gttgtcgttc gtgagtagcg ccctggagga aaccaccgga atcaaaggta cgttgcgtaa
    12121 cgtccatggt attcaggagg cgctcgcaca ggcaacgaaa aaagtcggca tcaatgttag
    12181 cgatatttcg cttattcgta tcaacgaagc aactccggtc attggtgatg tggcgatgga
    12241 aaccatcact gaaactatca tcaccgaatc aaccatgatc ggtcacaacc cgaaaactcc
    12301 gggcggagtg gggctgggcg tagggttcac catcactccg caagagttat taacacgccc
    12361 ggcggacaca ccttacatcc tggtggtgtc atcggcattt gattttgccg atatcgcgac
    12421 gatgataaac gcctccgtac gcgccggata tcagctgact ggcgttattc tgcaacgcga
    12481 cgatggcgta ctggtcaata accgcctgga aattccttta cctattgtgg atgaagtcct
    12541 ctacatcgat cgtattccgc tcggcatgct ggcagcaatt gaagttgccg ttcccgggaa
    12601 agttatcgaa accctgtcta acccgtatgg gattgccacc gtattcgccc tcaatgctga
    12661 agagactaaa aatattgttc ctgtcgcgcg ggcgctaatc ggcaaccgtt cagcagtggt
    12721 agtgaaaacg ccctctggcg acgttaaagc ccgttcgata cccgccggaa atatcgaact
    12781 gctttctgcc gggcgcacaa cgcgcgttga tgtagctgct ggtgccgatg ccattatgaa
    12841 agcggtcggt gagtgcccaa aactggaaaa cgtgacggga gaaccaggca ccaacatcgg
    12901 cggaatgctg gaacatgttc gccagaccat ggcggaactg accaacaaac ccagcaacga
    12961 aatttttatt caggatttgc tggcgataga tacctctgtc cccgtcagcg taaccggtgg
    13021 tctggcaggt gaattttcac tggaacaggc tgtgggtatt gcctctatgg tgaaatccga
    13081 ccgtctgcaa atggcgatga tcgccagtga aattaagcaa aaactgcatg ttgatgttca
    13141 ggtcggaggc gcagaagcag aagccgccat tcagggggcc ttgactaccc ctggaaccac
    13201 acgtccactg gcaattctgg acttaggtgc aggctctacc gatgcctcca tcatcaatca
    13261 gagtggggaa atcgttgcca ctcaccttgc gggagcgggc gacatggtaa cgatgattat
    13321 cgctcgtgag ctgggcttaa atgatcgtta tctggcagaa gaaatcaaaa agtacccgtt
    13381 agcaaaagta gaaagcctgt tccacttacg ccatgaagac ggtagcgttc agttcttccc
    13441 tactccccta tccccccatg tatttgcccg cgtctgtgta gtgaaacccg acgaactggt
    13501 gcctattccc ggtgatttga cgctggaaaa agtacgcgct gttcgtcgca gtgcgaaaga
    13561 gcgtgtcttc gtaaccaatg ccctgcgcgc gctacgtcag gtaagccctg ccggaaatat
    13621 tcgcgacatt ccgttcgtcg ttctggtggg tggttcttcg ctggatttcg aagtaccgca
    13681 attggtcacc gacgcgctgg cacattatcg actggtcgcc gggcggggga atatccgtgg
    13741 tagcgaaggt ccgcgcaacg cggtagctac tgggctactc atcgcctggc acaaggagtc
    13801 gattcatgga aagtaaagag cgtgcaccag ccatcgttgt catggagatc ggtgactgta
    13861 tcactacatg ggatgaggtg cttcttggca ttgaagagga aggtattccc tttcgcattc
    13921 aacacattcc gtcaggtgaa gttatcgata gcgcctggct ggctgctcgt caatctcccc
    13981 tgttggttgg cattgcctgc gaccgggaag aactgattgt gcattacaaa aacttaccgg
    14041 catcagcgcc actgtttacg ctgacgtatc aacaagataa ccatacccgg cgcagcatcg
    14101 gcactaacgc tgcccgcctg gtaaaaggaa ttcccttccg ggaattccac tcataatcca
    14161 caggagaaac gctgtatgaa caacgcacta ggactggttg aaacaaaagg gttagttggc
    14221 gcaattgaag ctgcagatgc catggttaaa tccgccaacg tgcagctggt tggctatgaa
    14281 aagattggtt cgggtctgat tacggtgatg gtccgcggtg atgttggcgc agtaaaggcc
    14341 gccgttgatg cggggagcgc tgcggcaagc gccgttggcg aagtgaaatc ctgccatgtc
    14401 atcccgcgcc cacacagtga tgttgaggct attttaccta aatcggcata accccatttg
    14461 ctaaggagca cagcgtgaag caatcactgg gattacttga agccagtggt ctggctctcg
    14521 ccatcagttg cgcagacgct atggcaaaag ccgcgtccat cacgctggtt ggccttgaga
    14581 agaccaatgg ctcaggctgg acgctcatca aaatcaccgg tgacgtcgcc tcggttaatg
    14641 cggcgatcgc gacgggagcc agtttcgccg gacagcgaaa tggccttgtc gcacacaaag
    14701 tgattgcccg cccgggagac ggcattctgc cccacacaac tgaagcattg accgtgacgg
    14761 ttgaaacaag caaaacacca gcaacaaccg tcacctcaga gattatcagt tgtaatttgt
    14821 gtcttgaccc ggcatgtcag cggcaaaaag gtgagccccg ctcactttgc ctgtacgcca
    14881 ataagcgagg ggagtagcag tggataaaca aaccttgcag gaaacagtga gccagattct
    14941 ggatgagatg cgcgagcgcc ctatcccact gggcgtttcc agtcgccata tacatctttc
    15001 atcagcggat tatttgcgtc tttttcctga acaaccgatt agcgagaaaa agccgttaat
    15061 gcaacctggc caatacgctg ctgaacaggt tgttacgctg gagggaccaa aaggcaaatt
    15121 gcacaacgtt cgcttgttgg ggccgctacg caatgcaagc caggtggaaa tttcccggac
    15181 ggacgccaga acgttaggga tcaccgcccc gctacgcatg tcaggcgatc tcagcggcac
    15241 tccggggatt cgtttgataa gcccatttgc ggaactggag atcacatctg gcgtcattgt
    15301 cgcgcaacgg catattcata tgtctccgct ggatgccctg atttatggcg ttacgcatgg
    15361 cgataaagtg gcggttgcca ttgaaggtga tgagcgacga ctgatattcg aaaacgtcgc
    15421 tgttcgcgtc tctccggata tgcgtcttga aatgcatatt gataccgatg aggccaacgc
    15481 cgcaggcgct gatagccctg acgtttttgc cacactggtg tcagtgccat gaataacatg
    15541 ctgatgcaac gtattgttga cgaagtggtt tttcgcctta aacaacgtgc gggaaaaaca
    15601 ctcgtgctga ctgtttttca attgcgagac gccagcgttc aagagagcgt tcatcagtat
    15661 gcgtcattac aaatccgata tgtcgatctg ccgctgctgc gacaacttgc ggagaacgaa
    15721 acgtcagatc gagcatctat ccagatacac gaagcgttag cctggggaat gcacattcaa
    15781 ttgtcgttgc agcgtcattt cctgaacgcc atcgaattga agactctcgc ccgtctaccg
    15841 ctaagctggt gcgacgaaca agggcagccg atctaccttc atcataatcg acttttaagt
    15901 tatgccgata tcgcacagtt aagctcggga atactggtgt tacagcggaa atgttgtgtg
    15961 accgcactgg ctcgtgaagc ggcaattacc cgaaatattc agttaatcag gcaggagtaa
    16021 cctatgcatc tggctcgagt cactggcgcg gtagtatcca cgcaaaaatc accttctctg
    16081 atcgggaaaa aactgctgct ggttcgccgg gtcagtgctg acggcgaact gcctctcacg
    16141 ccattaaccg gcgacgaagt tgccgttgat tcagtcggcg caggcgttgg tgagttggta
    16201 ctcctcagca gtggttccag cgccagacat gtgttttcag gccctaacga ggccatcgac
    16261 ctggcagtgg tcggcattgt cgatacgctt tcgcgctaag gagctaaaag atggcaattt
    16321 atacccgaac aggtgattca ggaagtacgt ctttatttac cggacagcgc gtcagcaaaa
    16381 ctcatctacg tgtcgaaacc tatggcacgc tggatgaact gaacgcgaca ctcagtctgt
    16441 gttattgcgc caccgccatc gaaagccatc gcatcttact ggaagccatt caacaacaga
    16501 tattttggtt tagcgccgaa ctcgccagtg aaagcgaaca gccgtcagcg caacagcgtt
    16561 acatcggtac ggaagaaatt gccgcgttgg aaaacgcgat cgatagtgca atgaacgccg
    16621 tccccccagt tcacagcttc attctgcctg gtcgatgtga agccgcaagc cgcatgcatt
    16681 tcgcccgcac ggtggctcga cgggctgaaa gacggctggt tgaactgaca acggaaacca
    16741 ccgtccggaa tgttttgctg cactatatca accgactttc cgattgcctg tatgccctcg
    16801 cccgcgtgga agacaacgtt gctcatcaaa acctgatgat tcaggaaatc acaaaacgtt
    16861 atcacgaagc caaccacaca ccagcattga aggaacgcac aatgccgctc acttttcagg
    16921 atcttcacca gctcattcgt agtgcggcaa tgcgcgcgga tgaactgcat attcccgttg
    16981 tcatcagcat tgttgatgcc aacggaacag aaagtgttac ctggcggatg cctgatgccc
    17041 tgttggtcag cagtgaactg gcaccgaaaa aagcctggac cgccgtggcg atgaaaacgg
    17101 caacccacaa gcttgctgat accgtgcaac ccgacgcgcc gctttacggg ctcgaaagcc
    17161 atatgcaagg aaaagtcgtc acttttggtg gcggcttccc cctctggcgt gacggaaaat
    17221 tgcttggcgg gcttggcatc agcggcggta gcgttgaaca agacatggat attgcccaaa
    17281 gcgcaatggc ggcaattaac gtgggagtaa accaatgaat aactctgaat tagaaacact
    17341 gatccgcaat attctgcgtg agcaacttgt ccccgctaca ccacgaacgc aaagaaacgc
    17401 tattttccag actgtcgatg aagcggtctg cgcagcacac taggcctttt tacgctttca
    17461 acagtgcccg cttaaaaccc gtagcgccat tatcagcgcc attcgcgaag aattaatgcc
    17521 ccgccttacc gaactggcag aagaaagcgc caaagaaacg gggatgggaa ataaagaaga
    17581 taagtaccta aaaaacaaag cggcactcga taacacgcca ggaattgaag atttaaccac
    17641 cacagcacta accggtgatg gtggcatggt tttgtttgag tactctccgt ttggggtgat
    17701 tggctcggtc acgccaagca ccaaccccac ggaaaccatc attaacaaca gcatcagcat
    17761 gctggcagca ggtaatagcg tctacttcag tccacacccg ggtgccaaaa acgtttcgtt
    17821 aaaactgatt accacaatcg aagacatcgc gtttcgctgt agtggcattc ataacttgat
    17881 cgttaccgta tctgaaccga cctttgctgc caccgaacag atgatggcgc accctaaaat
    17941 cagcgttctg gcgattaccg gcggtccggg gatcgtcgcc atgggaatga aaagtggcaa
    18001 gaaagtgatt ggtgccggag cgggtaaccc tccgtgcatc gtcgatgaaa cggcggatat
    18061 tgtgaaagcc gccgaggaca tcatcaacgg cgcttcattt gattacaacc tgccgtgtat
    18121 tgctgaaaaa agcctgattg tcgtggcatc agtggcagat tatttaattc agcagatgca
    18181 aagttttggc gcacttcttc ttaactatga gcaaactgag aaattgcgtg caatttgcct
    18241 gccagacggt agcgccaaca aaaaactggt tgggaaaagc ccatcagcct tgctggaagc
    18301 ggcaggcctc cctttgcccg caaaagcgcc gcgcctactg attgctgttg tcgacgccga
    18361 tgatagttgg gttacttgtg agcaactaat gccaatgctg cccattgtga aggtcaagga
    18421 ttttgacagc gctctaaccc tggcgctgaa agtggaagac ggcttgcacc acacggcaat
    18481 tatgcactct cagaacgtct cacgactcaa ccttgccgcg cgcgttatgc aaacctcaat
    18541 atttgtcaaa aacggcccct cctgggcggg tattggcgtc ggcggcgaag gctttacgac
    18601 ctttaccatt gcaacaccga ctggagaggg tacaacgtca gcacggactt ttgcccgctc
    18661 acgacgctgc gtgttaacca gcggcttttc aattcgctag agaaacggaa atgaatactt
    18721 tttcactgca gacgcgttta tacagcggac caggaagtct caaggtactt agccgtttct
    18781 ctcatagaca catctggatt atttgcgatg cctttctggc acgctcacca cttattgaca
    18841 cgttgcatca ggcgctgccc gacagtaatc gtctcagtat ttttagcgaa ataacgcccg
    18901 ccccaactat ccagaccgtg gtgaaaggca ttgcgcaaat gcagacttta cgcccggatg
    18961 tagttatcgg ttttggcggc ggttctgccc tggacgccgc aaaagcaatt gtctggttcg
    19021 gtcgccagtg tggcattgaa attgaaacct gcgtagccat tcctaccacc agcggtatcg
    19081 gttccgaagt gaccagtgct tgtgtcatca gcgatcctga gaaagggatt aaatatcccc
    19141 ttttcgataa tgcactttat ccggatatcg cgatccttga tccatctttg gtcgtcagcg
    19201 tgccaccggc gataacggcc aataccggga tggatgtttt aacccatgcg cttgaagctt
    19261 atgtttctcc tcgcgccagc gacttcacgg atgcactggt cgaaaaagcc gtgcaaattg
    19321 tttttcaata cctgcccact gcagtgaaaa aaggcgattg tcttgccacc cgtggcaaaa
    19381 tgcataatgc atcgacgctt gcaggaatgg cctttagcca ggccggactc ggtttaaacc
    19441 atgcaattgc tcatcaactc ggcggacagt ttcatctgcc gcatggactg gcgaatgcgc
    19501 tcctgctcac ggcagttatc cgctttaatg ctggcgatcc gcgcacagcc aaacgctatg
    19561 cccggctggc gaaaacgtgc catttatgcc cggacaacgc caatgacacc gccagcctga
    19621 atgcacttat tcaacatatt gagcagctta atacaacctg tacgctgccg actcttgcca
    19681 atgcgctgaa agaaaaaaaa gcagaatggt caatacgcat accagatatg gttcaggcgg
    19741 cgctggcgga tgcaacgttg cgtaccaacc cgcgtgcagc tgatgcctcc gcgattgcag
    19801 aactgctcga ggagttgtta tgaacgtcgg attaatctcc tgcgacgacc agaccatccg
    19861 cgagcgagta caagcagcag gtgtcgtggg tgctggcggc gcaggttttc caacccatat
    19921 taagctacaa gcgcgtgctg acactttcct ggtgaatgcg gcggaatgcg aacccatgtt
    19981 gaaagtagat caacagttaa tggcgcagca agccagccgc ctgcttcgcg gtgttcacta
    20041 tgcgatgaaa gccaccgggg ccagtagcgg aatcatcgcg ctgaaagaaa aatatcaacg
    20101 ggcaataaac gcactcacac cactgttacc accggatatc cgcctgcata ttttgcccga
    20161 cgtttacccc gcaggagacg aagtgctgac catctggatg gcaactggtc gccgcgttcc
    20221 acccgctgca ttacccgtta gcgtcggtgt ggtggtcaat aatgttcaaa ccgtacttaa
    20281 tattgccaga gccgtcgagc aacagtatcc agtgacgcgc cgcacactca ccgttaatgg
    20341 tgcagtggcg aagccaataa cggtaaccgt ccccatcggc atgtctttac gtgaagtcct
    20401 ggcgctggcg ggcggcgcaa cagtggatgc tcctggtttt atcaatggcg ggccgatgat
    20461 gggaagtttg atcacatcgc tcgacacacc agtcagcaaa accactggcg gcttgctggt
    20521 tttacccaac tctcattcac tgatccaacg tcgaatgcag aatgaccgca gcgtacttgc
    20581 cgtcgcgaaa actgtttgtg agcaatgccg cttgtgtacc gatctctgcc cacgtcattt
    20641 aatcggtcac gaactggcac cccatttatt agtacgtgcg gttaactacc aacagctcgc
    20701 cacgccgcaa ctgttactga cagcgctcac ctgttcagag tgcaacgtct gcgccagtgt
    20761 cgcctgtccg gtaggaattt caccaatgcg cataaaccgc ctgcttaagc aggaattacg
    20821 cgcacaaaat ctccgctacg aaggtgcctt aaatcctgca gatcccatgg caaattatcg
    20881 actgattccc gtaaaacggc tggtaaccag acttggctta accccgtggt atcaggatgc
    20941 gccattgtct gaacaggtgc ctcagcccga aaaagtgacg ttattgttac gccagcacat
    21001 cggtgccagc gcaattgcgt gtgtacaaaa aggcgatcgc gtcgttcatg ggcaatgtgt
    21061 aggtcaaatt ccacacggca cattaggtgc ccccattcat gccagtattg atggcatggt
    21121 cagcgatgtg acagaaaacg cgattacgct tgtgagaggt taaccatgtc gaaagcggta
    21181 ggtattttag aacttagcag tatcgcgaaa gggatggaaa ccggcgacgc catgctgaaa
    21241 agcgccaacg ttgagttact ggtcagcaaa acgctatgcc ccggaaaatt tctgctgatg
    21301 ctcggtggcg atgtgggcgc ggtgcaacag gccatcaaca gcggagcgtc ccttgctggt
    21361 gaaatgctgg tcgacagtct tgtcctgccg aatattcatc ccagcctcct ttctgcaatc
    21421 agcggactta accatgttga acagcaccgg gctgtcggcg tagtcgaaac ctggagtgtc
    21481 gccgcctgca ttgacgctgc cgattgtgct gtcaaagctg caaatgtcat tcttgttcgc
    21541 gtacatatgg cttttggcat tggcggtaaa tgctacatgg tggttgctgg cgatatttca
    21601 gatgttaata acgcggtctc tgccgccagc gtgcgcgcgg gggaaaaagg tttgttggtc
    21661 tatcggtcgg tgattgcccg tccacatgaa gccatgtggc gtcagatggt ggagggataa
    21721 tgacaactca gcacacaact gaacggatga ttcaggaata tgtcccaggt aagcaggtca
    21781 cgctggcaca tgtcatcgcc aatccaggga aggatctgtt taagaaattg ggtttaccag
    21841 acaccatttc cgctattggt attctgacaa ttacccccag cgaagcatct atcatcgcat
    21901 gcgatatcgc cacaaaatca ggcgctgtag agatcggttt tcttgatcgt tttaccggtg
    21961 ccgtagtgct gactggtgat gtttctgcga tcgaatatgc acttagccag gttacccgta
    22021 cgctgggtga aatgatgcgc tttaccgcct gccccattac gcggacctga cgttatgcaa
    22081 cgtatgatgc tcatcggccc cagccagtgc ggaaaaacgt cactcacgca atgtctgcgt
    22141 ggcgaaaata ttcattatca gaaaacgcag gcaatcgtct ggtccccgac caccattgat
    22201 acaccaggcg aatacctcga aaaccgcagc ctctacagtg cactgttggt cagtgcctgc
    22261 gaagcggaca ttatcgcgct ggtgcttaat gcggacgcac cgtggtcacc tttttcgcca
    22321 ggctttacct cgtcaatgaa tcgcccggtc attggagtag taaccaaagc agatttagcc
    22381 aacatggaac aaatatcttt ggtgaaatgt tggttacggg aagctggagc gcacaacgtg
    22441 ttagtaacca gtgcggttaa taaccatgga gttgcagaac ttttagcctt gctgcataca
    22501 gaagaaggct gtcgctaatt tcgacaggtt atcagatggg aatgatgacg gtgatacaca
    22561 gaaaagaatc aatgagcaat gagggttgcc gggcaaccct cattgaataa aacaggaatt
    22621 acttcgcttc gccgtttttc atttcgccca tagctttcag ctttttggag atatcgcggc
    22681 gttctttgga gagctcggca tttttgatga tgtaatcgtc aacgcgatct tcatagtcac
    22741 ctttcatgct ggcgatgatg ccctggattg cttcaacgct catcccaggc ttgatgtaat
    22801 cgctcaggtt gtccagcagc aggacgcgct tctggttgtc acggatcttt ttctcaacgt
    22861 cctgaatttc acgttgcagt ttgttcttac gacggaacag acgaacaaat tccagtacgt
    22921 cctggaatga aggcttggta gtttccattt ttatacccct gataatgtga gagtcagatt
    22981 cgtttaagca accgctattc gttagggcca acattactca tagctgccgc agatgacaat
    23041 gcttttatcc cttttcacta tcataccctt tatccttgct gaatcgaagc agcagcaaga
    23101 tgattctgaa gttcaggaat tatttgcgca aggcccggca aatcagattc gatagcaact
    23161 caagctgatg cgccgtttcc atgttcagcc acacataacc atagattggt gtttccacta
    23221 ccttcaggtc atggtggtta ttgagcaact gacgcagctc ttccacagca tcgttcaatt
    23281 tttccgtatt ggcaaaaacc ggctgcggat taccttcgta cagcgcatga acaaagctca
    23341 gcagtatttg ctgcatcatg tgctgggtat cacgaagttt ttgcgcgttc aataacacga
    23401 aatggctggg gcgcgtggcc cagtacgcat tgatttgcaa ctccagcata caaaccagat
    23461 tgcggttaat ggtctggata ccttcatata tcgattttgg aatacgggtt tctttgctgg
    23521 cgggcgcaat cagcccacgc attttcacgg catcggtcag gagtttttgt agatggcttt
    23581 ccagacgtgg gcgttcgagt aagttcggtg agaatgcaga ttgatagacc cgattatact
    23641 cggtcagact tttcgccagt tgaatgcgcc agtggatgaa cgcccgttgt ggccagatac
    23701 cggtaaacaa cattgccagt aaagagccga ggatcacatc gccgcttcgc cataacgccg
    23761 tatcaatttc acctgtcggg gaaccaacaa caattgccag cgtcacccca atcaataatc
    23821 cttgatacgg tttcttgccc agcgccagcc aaccgcaaag aaacatcgcc gccgcgcacc
    23881 agactaacat cagcggtaac gaaattaact ccagttgcag agcgataaga cctaaaatcg
    23941 aacccaacac cgtaccgcca atacgttcga aggcacgagg gacaacgtta ccccagaacg
    24001 agattggccc cataatcacc accatggtga ccagcggcca ggtgctttcc gggatagtaa
    24061 acaggcggat aatgagaaaa gtgagcagga atgccagcgc gacccgagta ccatgcacta
    24121 tgcggtaatg gcggtatacc cggatttcaa acgggcttaa tgacttatcg gcacgcacgc
    24181 agaaactccg taatgaagat taaaagcgct cataactcat ctgtcggcca gaaggcgcgc
    24241 ctctgaatta tccgacggac aaaccgggaa cagtgtaatt tcccggcagg agcgaaaaaa
    24301 ttgaaaggcg catcttactc ttttcgcttt caaaaaaaga gtggtcatcg cgttaacaca
    24361 ccgccctgag atgaattagt gatgttttgg ctgcaccgca acatacattt cgatatccca
    24421 gtacccatct tccgcgccat tgttcaaata aacctcgaag cagggctttg gtaacatttc
    24481 ataagcactg tcctgcaaga ggctattaaa gaactgatac cagggtttag caaaatcatc
    24541 accgactaca cgagccaccg ccaccgcata ctgaccacct gaaatttctg tcagaatgac
    24601 gccctcactg ttttcgggaa gcgtaaagtt attcggtacc gtcacaacgg tgtcgcagcg
    24661 taatttttcg gcgggtgttt catctggatt gtcgtaatag acagcaaccc actccttcgg
    24721 cacaatattt ttgttatcta cccacatcat caactgctca aagcctttct ttaccgtctg
    24781 ttcccacggg ccaacgagat ggaaacctgc aacggtacgt ttctcttcct gcgtaatctc
    24841 gtagttcatg acgcctccat taacactgtt tttatataca gcatagttgc aatattaaaa
    24901 ccacaaggaa tgagtgttga ttatgcgagc agactcgcac tcctgccagt ctgctgcaaa
    24961 agaaaggtca ggccttatgg tggaaataat cactcaggcg ggaaaacatg ccgccttccc
    25021 cgacagattc cagggtaacc agcggccagt gcgccacctg tttatcacgg tcgtaaagtt
    25081 caatttcccc tacccgctga tgggcgctaa ttggcgcggt gagctcttta ccatcaaggg
    25141 tatatttggc tttgatatgt ggaatttcgg ctttcggtag caccatccag aactcttgtt
    25201 ccgttcctag gtcgatattt tctttatcgc cataccagat gcgttccgtt ccgacctttt
    25261 tcccacggtg caaaatttgc accgtagtaa agttttgttg cccccaacgc agtaattttc
    25321 ttgcctcttc ctcacgacct tttgcactgt cagcccccat taccactgca atgagacgac
    25381 gctgcccatc tacagccgaa gcaatgagat taaacccggc accagaagta tgacccgttt
    25441 tcaggccgtc aacattcatg gttttatccc acaacaaccc gttacggttt tgctgggtga
    25501 taccgttcca ggtgagactt ttctcactgt acatatgata aaactcgggc tcgccgtgga
    25561 tgatagcgcg agaaagcaca gctaaatcat aagccgagct atgctggcca ggtgcatcca
    25621 gaccatgcac tgtttcaaaa tgcgtatcct tgagatgcag cttctcggca tagttgttca
    25681 tcatttcaac aaactgccgt tgcccaccgg caatatagtc agccagagca acacaagcgt
    25741 catttccgga atccacaatt aaaccacggc ttaaatcacg taccgatacg cgatcgccct
    25801 ctttcaaaaa catcagtgaa gaaccgacaa acaccggatt atctttcgcc cacgcatcgc
    25861 gccccacggt gacaatatcg tctggcgtaa tgcgatgact atcgatagcg cgatccacga
    25921 cataacccgt catcagcttt gtcaggctgg cgggattgcg ctgttgatgc tcattacccg
    25981 cggtgaggat ctgaccggtg gtgtaatcca tcaataccca ggacccggca tgaatctctg
    26041 gaggctgagg tgaaaaagga atgttttccg ccgcaaaacc agacgataag ttaaaaacga
    26101 acaaagaagc agcaataata agacggcgtt tcaacagcaa accctcagga gtttcaaata
    26161 gctgttcttt ttacggaaat acttatgaac tggctggaat aaagtgcaag aaaatgtgac
    26221 taccctctca tttttatctg acatgatctg ttgccactcg ctgccaaatt gtggcgctaa
    26281 agctgattag cacggtgata tttgatactc tggcagacag cagaaataac ggatttaacc
    26341 taatgatgaa tgacggtaag caacaatcta cctttttgtt tcacgattac gaaacctttg
    26401 gcacgcaccc cgcgttagat cgccctgcac agttcgcagc cattcgcacc gatagcgaat
    26461 tcaatgtcat cggcgaaccc gaagtctttt actgcaagcc cgctgatgac tatttacccc
    26521 agccaggagc cgtattaatt accggtatta ccccgcagga agcacgggcg aaaggagaaa
    26581 acgaagccgc gtttgccgcc cgtattcact cgctttttac cgtaccgaag acctgtattc
    26641 tgggctacaa caatgtgcgt ttcgacgacg aagtcacacg caacgttttt tatcgtaatt
    26701 tctacgatcc ttacgcctgg agctggcagc atgataactc gcgctgggat ttgctggatg
    26761 ttatgcgtgc ctgttatgcc ctgcgcccgg aaggaataaa ctggcctgaa aatgatgacg
    26821 gtctaccgag ctttcgcctt gagcatttaa ccaaagcgaa tggaattgaa catagcaacg
    26881 cccacgatgc gatggctgat gtgtacgcca ctattgcgat ggcgaaactg gtaaaaacgc
    26941 gtcagccacg tctgtttgat tatctcttta cccatcgtaa taaacacaaa ctgatggcgt
    27001 tgattgatgt tccgcagatg aaacccctgg ttcacgtttc cggaatgttt ggggcatggc
    27061 gcggcaatac cagctgggtg gcaccgctgg cgtggcatcc agaaaatcgc aatgccgtaa
    27121 ttatggtgga tttggcagga gacatttcgc cattactgga actggatagc gacacattgc
    27181 gcgagcgttt atatactgca aaagccgatc ttggcgataa cgccgccgtt ccggttaagc
    27241 tggtgcatat caataaatgt ccggtgctgg cccaggcgaa tacgctacgc ccggaagatg
    27301 ccgaccgact gggaattaat cgtcagcatt gcctcgataa cctgaaaatt ctgcgtgaaa
    27361 atccgcaagt gcgcgaaaaa gtggtggcga tattcgcgga agccgaaccg tttacgcctt
    27421 cagataacgt ggatgcacag ctttataacg gctttttcag tgacgcagat cgtgcagcaa
    27481 tgaaaattgt gctggaaacc gagccgcgta atttaccggc actggatatc acttttgtcg
    27541 ataaacggat tgaaaagctg ttgttcaatt atcgggcacg caacttcccg gggacgctgg
    27601 attatgccga gcagcaacgc tggctggagc accgccgcca ggtcttcacg cccgagtttt
    27661 tacaaggtta tgcagatgaa ttgcagatgc tggcacaaca atatgccgat aacaaagaga
    27721 aagtggcgct gttaaaagcg ctttggcagt acgcggaaga gatcgtctaa tttgaagcct
    27781 ccgccgctgg tacggcggag gtcaatgttg agtcaggctt tttgaacggt gatactccac
    27841 gctgcatcgc caatctgctg ataatcggtg atggcatgcc cttcttctgc cgcccactgt
    27901 gggattgctt cagttgcctg ggtgcaatca aactcaatta ccaactcatc gccgctggcc
    27961 atttctgcca gtgctgcttt tgcttctatg agtggaaacg gacagacctg cgtcaccaca
    28021 tccagctttt taatcgccat ctttacacct tacttaattt gccgcagcag ttgccagtcg
    28081 cgccttacgc tgcggtcgaa catacacaag ccaggacgca gtccagactc cgagaatcat
    28141 aaatacaagg ccaatccagc cctgccaggt catcattgca gtcataacca gtccattacc
    28201 gataaagcaa ccacctgcaa tactggcacc gaagcccatc agtacaccac ctaacccgct
    28261 acgtaatgtt gtttgtgcat cagctgcgcg aacgcggaac tcacggctcg ctttggctgc
    28321 aataaatgac cccacgaaga tccctaacac caggaaaacg ccccagttaa tgtatttcac
    28381 gtcacccgcg accagaaatt gcagaatatt ggccgttggg gaagtgattc ctaacccaaa
    28441 catgcgtccg gttgcttcac tcaggggcca ggctaaaagc gcaatcaaac cgatgagtac
    28501 agcagtgaca aagggatgcc agcgtttttc aaacagaata tgagcgatcc cagttcggcg
    28561 cggcggtaag gtcgcgactt tgagttttgg cttcttcaac tctttcatca ccacccagag
    28621 cgttatcacc agcaaaacgg caaccaacgg ccaaacagac aaattaaaag tctcagcaat
    28681 agagttatgt tcagtactgt agtgctgcaa ggtttgattt aaaccactgg catgtggaga
    28741 acgcatcacc gcactcatca ccatataagt gaaaagcgcg atccaactgc cgatcaatcc
    28801 ctcacccgcg cgataccagg tcccggtggc acatccaccc gccagaacaa ttcccagccc
    28861 gaagatatac ccacctataa cagtaccaag ccacgggaac gcctcggctt cgtaagtcag
    28921 taggcccgcc tgaattaacg caaaaacccc cacgctttgt accgaaattg caatcagcaa
    28981 ggcgtaaaac atgcgattat ttttcgcgat atacatatcg cgaaaaccac ctgtcaggca
    29041 aaaacgccca cgctgcatga caaatcccag cagagcacca caaattagcc cgcttaatat
    29101 cattgaaaac ataaattaat taaccagatg aatgttaatg aggaaattat tcatgactgg
    29161 tggaatgcag accaataacc aaattctcta aattagaaca aatggttatt aatgaggatt
    29221 tcgataaaaa aaataaaaaa accggagcca aatattcatt cgactccggt tgattattag
    29281 aaaagatgat tatgcaacgt cttcgtactg cggaactggg ttacggaagc tcttggtgac
    29341 gcaagccagg taaatcagac cgatagcagc ccaaatcaga cccagaacca ttgagctttc
    29401 ttccaggtta acccacagcg caccaacggt cagcgcacca cacatcggca ggaacagata
    29461 ctggaagtga tctttcagcg tcttattacg cttctcacgg atccagaact gcgagatgac
    29521 cgacaggtta acgaaggtga acgccaccaa cgcaccaaag ttaatcagcg ctgtagccat
    29581 taccaggtcg aagttgattg ccagcagggc aatcgcgcca accaggatga tgttcatcgc
    29641 cggagtacgc catttcgggt gtacataacc gaagaagctt ttcgggaata cgccgtcacg
    29701 acccattacg tacatcagac gcgctacgcc tgcatgcgcc gccataccgg acgccagtac
    29761 ggtaatggtg gagaagatca gcgcgccaac ctggaacgct ttacccgcaa cgtacagcat
    29821 gatttcaggc tgtgacgcat ccggatcttt aaagcgagag atatccggga agtacagctg
    29881 caggaagtaa gttgcaaaga tgaagatcat gccgccaatc agcgcggtca ggaaaatcgc
    29941 acgcgggatc acgcgctctg catctttggt ttcttccgac aggttgctga tgccgtcaaa
    30001 gccggtaaag gaaaagcaca ggattgtcgc cccggtaatc atcggaataa catgcgcatc
    30061 gccagaccag aacggacgag tgctcgccag cgtaccagcg ccttcacctt caaatacgcc
    30121 ataaacaacc atgcccagaa tcaccgcgat cagcactacc tgcaacacga cgattacagt
    30181 gttgaagttc gctacggatt tcagactacg caggttaaag gcggtcataa aggccaccag
    30241 cgccaccaca aacatccacg atgggatgga aggcaccaga gcttcaaaat agattttcgc
    30301 cagcagaatg ttgatcatcg gcgcgaacag atagtcgagc agagaagacc aacccaccat
    30361 aaagccgaca gtcgggctaa tggatttctg ggcgtaagta tatgcagagc cagcagaagg
    30421 atagcggcga accagcttcc cgtagctcag agccgtaaac aggatcgcaa tcaatgcgaa
    30481 cgcataggct gtcggcacat gaccatccgt gaggcctgaa acgataccaa aagtatcaaa
    30541 cagcgtcatc ggctgcatat aggcaagacc catcattaca accggaacta acgtaagcgt
    30601 tttacgcaat tccacgcgag aggtgtttgg agtaacgtta tgcgacatgg tcattctcct
    30661 ttacggtgat aaccgtcgcg taagcaaaaa attgccccat ttttttggat tcctcagcga
    30721 caacaactgt cgatttttag taaatatcta tccggtacga agcccggcct cttggtatga
    30781 atgattggtt tgaagcaaaa aataaccgac gctgatgaaa cgtcggtttt tagtcatttt
    30841 ttgacagcgg cgcattgtgc ctaaatgggg gggaaatgac aagagaatga gaagcttgtc
    30901 ataataattt ttctttaaat ggctgatttt ccgtcatcag attttgcata aacaccggcg
    30961 atcgcactat ttgctaaaat ttcatcccgc caccagccgc tggcaagccc tgcggtctgt
    31021 tcgttccagc caatccagac gggttcaaag agagtttggg cgaccacttt cttctcgatt
    31081 aacgcgccac tatcgagaaa acgttgcacc agataacggg gtaaatagcc gcagccgagg
    31141 ccgctaattt gcagctccag cttggtttta aaatcgaaaa cggtaatggc ttcttgttcg
    31201 tcgagaagtt gtgaggccgt agaagcgcct gcctgcgcag tgtcccccac cacaatcgcc
    31261 cgatatcgct taatgatacg acggtttaac ggctcttctt cctgggctaa cggatgatga
    31321 ggcgcgaccg caaagacttg ttccagatcg cccaaccgcg agaaaccaaa ctcactggaa
    31381 gatggtggct catgcatcgc gccaacaata atatccgctc gcccctgagt cagggcatcc
    31441 caggaaccac cgagtacgcc attgataaac ttcaggcgcg tcacgctatg atgttgataa
    31501 aaagcttcaa tcagtggcgc aagcagagaa aaaggaaacg tgtcgtccac gccaatcacc
    31561 agctcgtttt cccagccttc atgcagtttg atcgcctgct tttccagttc tcgcacggta
    31621 tgcagaactt cccgcccctt ctctaataac atttttccgg tacgggtgaa tttagcgcgg
    31681 tgaccgctac gatcaagtaa ttgaatatta aggtcgcttt ccagcttgtg aacggtgtaa
    31741 ctcagagcgg acggcgtctt gtagagttta gccgacgccg ccgcaaaact gccttctttt
    31801 tctaaggcat cgaggatcat caacacatcc agcagttgtt tcatacacgc ccccttgcag
    31861 tgcatgcggc gtcctgctgg cgtgatcgtt tactccagcg gcattaccag cggatcggga
    31921 tactggtatt caaatcccag ttcattacaa atccgactgc catcaataat cttacctttg
    31981 ccgctgtcca gactatttct gaactgcgga ggttccagcc ccagtaaacg ggccatctgc
    32041 ggatagaaaa cattacgcgc agggtgagct ggcgcacata tattatagat gtgtccgcct
    32101 ttaggtgcct gtaacaacag agtgatagcg ccaataacat cttctaaatg gactaaatta
    32161 acaccatgtt caccatcagg cgcggttttt ccggcaaaga agcgtccggg atgacgtccc
    32221 ggtccgacca gccccgcaag acgcagaata tcgaccgaag taccgggtaa attgtgcagc
    32281 cagtcttcga gttcttctaa tactcgtcca ctgttggtca ccggattacg cggggtggtt
    32341 tctttcaccg tgccttgcgc gtcgccatag acagacgttg agctggtaaa aataatgcga
    32401 ggaatacgat gggccagcgc gctatccact aactcttgta ccgcttgtaa atagaactca
    32461 tcgccggggc cgctacgacg tgccggaagc gtaatgacca gcgcatcggc atccatcagg
    32521 gcatccagat cgtcagaatc gcaaactaac tcaggctcca tgcgaagcag atagctatca
    32581 atgccactca ttcgggccgc ttcgacacca tcttgtgtgg ttttactccc ggtgacttgc
    32641 cagcctcgcg ctgaaagtga catcgccagc ggcatgccta accaccctaa cccgacaatt
    32701 gcgacctttt tcatccgtta tctcctgata caccttagat ctataaggct acgctagcgt
    32761 atcaaaactg acaattcatt ctatgaatga atctgttcaa taatgataac gacatgctgc
    32821 aatgagcagt gaatcgtcgg taaccgcgta taccagacgg tgctcctctg taatgcgtcg
    32881 ggaccagaaa cctgacaaat tatgtttcag gggttctggc ttccccttac cttcaaatgg
    32941 cgttctgcgg gtatctttga taagttcatt gatcttttta acaattcgct tatctgtttc
    33001 ctgccagtac agataatcat cccatgattc ctcagaccag attagtttca ctcaataatg
    33061 tccttttctg ttcctttgcc tgatttcagg ctatcgattg agtccatcaa tctccgggcg
    33121 ttagcggggg agcgcagtag ataagccgtc tcttccagcg agttgtattc ttcgagtgac
    33181 atcagaacac aagcctctcc attctgacga gtaataagga tcggggcatg atcttcaacg
    33241 gctttcatca ttgttgccga caaattctga cgcgcttcgc tgtagctaat tgtacgcatg
    33301 tcaatctcct cttttgtaca gtttattgta caatgatggg cgttaattaa ctatttatta
    33361 attagtttgt agatcaaggt attgtcagtg agacgaaaat ccaggcttcg ctatttttgg
    33421 tgccgtcagc ttagaggaca gtcctcttag ccccctcctt tccccgctca ttcattaaac
    33481 aaatccattg ccataaaata tataaaaaag cccttgcttt ctaacgtgaa agtggtttag
    33541 gttaaaagac atcagttgaa taaacattca cagagacttt tatgacacgc gttcaattta
    33601 aacaccacca tcatcaccat catcctgact agtctttcag gcgatgtgtg ctggaagaca
    33661 ttcagatctt ccagtggtgc atgaacgcat gagaaagccc ccggaagatc accttccggg
    33721 ggctttttta ttgcgcggtt gataacggtt cagacaggtt taaagaggaa taacaaaatg
    33781 acagacaaca ctcgtttacg catagctatg cagaaatccg gccgtttaag tgatgactca
    33841 cgcgaattac tggcgcgctg tggcattaaa attaatcttc acacccagcg cctgatcgcg
    33901 atggcagaaa acatgccgat tgatattctg cgcgtgcgtg acgacgacat tcccggtctg
    33961 gtaatggatg gcgtggtaga ccttgggatt atcggcgaaa acgtgctgga agaagagctg
    34021 cttaaccgcc gcgcccaggg tgaagatccg cgctacttta ccttgcgtcg tctggatttc
    34081 ggcggctgcc gcctttcgct ggcaacgccg gttgatgaag cctgggacgg cccgctctcc
    34141 ttaaacggta aacgtatcgc cacctcttat cctcacctgc tcaagcgtta tctcgaccag
    34201 aaaggcatct cttttaaatc ctgcttactg aacggttctg ttgaagtcgc cccgcgtgcc
    34261 ggactggcgg atgcgatttg cgatctggtt tccaccggtg ccacgctgga agcaaacggc
    34321 ctgcgcgaag tcgaagttat ctaccgctcg aaagcctgcc ttatccagcg cgatggtgaa
    34381 atggaagaat ccaaacagca gctgatcgac aaactgctga cccgtattca gggtgtgatc
    34441 caggcgcgcg aatcaaaata catcatgatg cacgcaccga ccgaacgtct ggatgaagtc
    34501 atcgccctgc tgccaggtgc cgaacgccca accattctgc cgctggcggg tgaccaacaa
    34561 cgcgtagcga tgcacatggt cagtagcgaa accctgttct gggaaacgat ggaaaaactg
    34621 aaagcgctgg gtgccagttc aattctggtc ctgccgattg agaagatgat ggagtgatcg
    34681 ccatgagctt taacacaatc attgactgga atagctgtac tgcggagcaa caacgccagc
    34741 tgttaatgcg cccggcgatc tccgcttctg aaagcattac ccgcactgtt aacgatattc
    34801 tcgataacgt gaaagcacgc ggtgatgacg ccctgcggga atacagcgcg aagtttgata
    34861 aaaccacggt taacgcgctg aaggtgtctg ctgaggagat tgccgccgct agcgaacgcc
    34921 tgagcgacga gctaaaacag gcgatggcgg tggcagtaaa aaatattgaa accttccaca
    34981 ctgcgcaaaa actgccgccg gtagatgtag aaacgcagcc aggcgtgcgt tgccagcagg
    35041 tcacgcgccc ggtcgcttca gttgggttgt atattcctgg cggctccgcc ccgctcttct
    35101 caacggtatt aatgctggcg actccggcgc gtattgcggg ctgtaaaaaa gtggtgctgt
    35161 gctcaccgcc gccgattgcc gatgagatcc tttatgcggc gcagctgtgc ggtgtgaagg
    35221 acgtgtttaa cgtcggcggc gcacaggcca ttgccgcgct ggcgtttggt acggaatctg
    35281 tgccgaaagt ggacaaaatc ttcgggccgg gtaacgcctt tgtcaccgaa gcgaaacgtc
    35341 aggtgagcca gcgtctggac ggtgcggcta tcgatatgcc cgcaggcccg tcggaagtgt
    35401 tggtcattgc cgacagcggc gcaacgccgg atttcgtggc ttctgatttg ctttctcagg
    35461 ctgaacacgg cccggactca caggtgattt tactgacgcc tgacgctgat atggcgcatc
    35521 aagttgccga agccgtcgaa cgccagttag cagaactgcc gcgtgccgaa accgcccgcc
    35581 aggcactgaa cgccagccgc ctgatcgtga ctaaagattt agcgcagtgc gtagagatct
    35641 ccaaccagta cggcccggag cacctgatca tccagacccg caacgcccgc gatctggtcg
    35701 atggcatcac cagcgccggt tcggtatttc ttggtgactg gtcaccggaa tcggcaggtg
    35761 attacgcctc cggcaccaac cacgttctgc cgacttacgg ttacaccgcc acctgttcca
    35821 gcctcgggct ggcagatttc cagaagcgta tgaccgtgca ggaactgtcg aaagaaggtt
    35881 tctccgcgct ggcttcgacc attgaaacat tggccgccgc cgagcgcctg accgcccata
    35941 aaaatgccgt tactttgcgt gttaacgccc ttaaggagca agcatgagca ccgtgactat
    36001 taccgattta gcgcgtgaaa acgtccgcaa cctgacgccg tatcagtcgg cgcgtcgtct
    36061 gggcggtaat ggcgacgtct ggctgaacgc caacgaatac cccactgccg tggagtttca
    36121 gcttactcag caaacgctca accgctaccc ggaatgtcag ccgaaagcgg tgattgaaaa
    36181 ttacgcgcaa tatgcaggcg taaaaccgga acaggtgctg gtcagtcgtg gcgcggacga
    36241 aggtattgaa ctgctgattc gcgctttttg cgaaccgggt aaagacgcca tcctctactg
    36301 cccgccaacg tacggcatgt acagcgtcag cgccgaaacg attggcgtcg agtgccgcac
    36361 agtgccgacg caggacaact ggcaactgga cttacagggc atttccgaca agctggacgg
    36421 cgtaaaagtg gtctatgttt gcagccccaa caacccgaca gggcaactga tcaatccgca
    36481 ggattttcgc actctgctgg agctaacccg cggaaaagcc attgtggttg ccgatgaagc
    36541 ctatatcgag ttttgcccgc aggcatcgct ggctggctgg ctgacggaat atccgcacct
    36601 ggctattttg cgcacactgt cgaaagcttt tgctctggcg ggccttcgtt gcggatttac
    36661 gctggcaaac gaagaagtca tcaacctgct gatgaaagtg atcgccccct acccgctctc
    36721 gacgccggtt gccgacattg cggcccaggc gttaagcccg cagggaatcg tcgctatgcg
    36781 cgaacgagtg acgcaaatta ttgcagaacg cgaatacctg attgccgcac tgaaagagat
    36841 cccctgcgta gagcaggttt tcgactccgc aaccaactac attctggcgc gctttaaagc
    36901 ctccagcgca gtgtttaaat ctttgtggga tcagggcatt atcttacgtg atcagaataa
    36961 acaaccctct ttaagcggct gcctgcgaat taccgtcgga acccgtgaag aaagccagcg
    37021 cgtcattgac gccttacgtg cggagcaagt ttgatgagtc agaagtatct ttttatcgat
    37081 cgcgatggaa ccctgattag cgaaccgccg agtgattttc aggtggaccg ttttgacaaa
    37141 ctcgcctttg aaccaggcgt gatcccggaa ctgctgaagc tgcaaaaagc gggctacaaa
    37201 ctggtaatga tcaccaatca ggatggtctg ggaacacaaa gtttcccgca ggcggatttc
    37261 gatggcccgc acaacctgat gatgcagatc ttcacctcgc aaggcgtaca gtttgatgaa
    37321 gtgctgattt gtccgcacct gcccgccgat gagtgcgact gccgtaagcc gaaagtaaaa
    37381 ctggtggaac gttatctggc tgagcaagcg atggatcgcg ctaacagtta tgtgattggc
    37441 gatcgcgcga ccgacattca actggcggaa aacatgggca ttaatggttt acgctacgac
    37501 cgcgaaatcc tgaactggcc aatgattggc gagcagctca ctaaacgaga ccgttacgct
    37561 cacgtagtgc gcaacaccaa agagacacag attgacgttc aggtgtggct ggatcgcgaa
    37621 ggtggcagca agattaacac cggtgttggc ttctttgatc acatgctgga tcagatcgcc
    37681 acccacggtg gtttccgtat ggaaatcaac gtcaaaggcg acctctatat cgacgatcac
    37741 cacaccgtcg aagataccgg cctggcgctg ggcgaagcgt taaaaatggc tcttggcgac
    37801 aaacgcggta tttgccgctt tggttttgtg ctaccgatgg acgaatgcct tgcccgctgc
    37861 gcgctggata tctctggtcg cccgcacctg gaatacaaag ccgagtttac ctaccagcgc
    37921 gtgggcgatc tcagcaccga gatgatcgag cacttcttcc gttcgctctc atacaccatg
    37981 ggcgtgacgc tacacctgaa aaccaaaggt aaaaacgatc accaccgtgt agagagcctg
    38041 ttcaaagcct ttggtcgcac cctgcgccaa gccatccgcg tggaaggcga caccctgccc
    38101 tcgtcgaaag gagtgctgta atgaacgtgg tgatccttga taccggctgc gccaatctga
    38161 actcggtgaa gtcagccatt gcgcgccacg gttatgaacc aaaagtcagc cgtgacccgg
    38221 acgtggtgtt gctggccgat aaactatttt tacccggcgt tggcactgcg caagcagcga
    38281 tggatcaggt gcgtgagcgc gagctgtttg atctcatcaa agcctgtacc caaccggtac
    38341 tgggcatctg cttagggatg caactgctgg gtcggcgcag cgaagagagc aacggcgtcg
    38401 acttgctggg catcattgac gaagacgtgc cgaaaatgac cgactttggt ctgccgctgc
    38461 cgcatatggg ctggaatcgc gtttacccgc aggcaggcaa ccgcctgttt caggggattg
    38521 aagacggcgc gtacttttac tttgttcaca gttacgcaat gccggtcaat ccgtggacca
    38581 ttgcccaatg taattacggc gaaccgttca ccgcggcggt acaaaaagat aacttctacg
    38641 gcgtgcagtt ccacccggag cgttctggtg ccgctggcgc taagttgctg aaaaacttcc
    38701 tggagatgtg atgattattc cggcattaga tttaatcgac ggcaccgtgg tgcgtctcca
    38761 tcagggcgat tacggcaaac aacgcgatta cggtaacgac ccgctgccgc gcttacagga
    38821 ttacgccgcg cagggtgccg aagtgctgca tctggtggat ctgaccgggg caaaagatcc
    38881 ggcaaaacgt caaatcccgc tgattaaaac cctggtcgcg ggcgttaacg ttccggtgca
    38941 ggttggcggc ggtgtgcgta gcgaagaaga cgtggcggcg ttactggaag cgggcgttgc
    39001 gcgcgtggtg gttggctcca ccgcggtgaa atcaccagaa agggtgaaag gctggtttga
    39061 acgtttcggt gccgatgcct tagtgctggc gctggatgtc cgtattgacg agcaaggcaa
    39121 caagcaggtg gcggtcagcg gctggcaaga gaactcgggc gtttcactgg aacaactggt
    39181 ggaaacctat ctgcctgtcg gcctgaaaca tgtgctgtgt accgatatct cgcgcgacgg
    39241 cacgctggca ggctctaacg tctctttata tgaagaagtg tgcgccagat acccgcaggt
    39301 ggcatttcag tcctccggcg gtattggcga cattaacgat gttgctgccc tgcgaggcac
    39361 tggcgtgcgc ggcgtaatag ttggtcgtgc attactggaa ggtaaattca ccgtgaagga
    39421 ggccatcgca tgctggcaaa acgcataatc ccatgtctcg acgttcgtga tggtcaggtg
    39481 gtgaaaggcg tacagtttcg caaccatgaa attattggcg atattgtacc gctggcaaaa
    39541 cgctacgccg aagaaggtgc agacgaactg gtgttctacg atatcaccgc ttccagcgat
    39601 ggccgtgtgg tagataaaag ctgggtatct cgcgtggcgg aagtgatcga cattccgttc
    39661 tgcgtggcgg gtgggattaa gtctctggaa gatgccgcga aaattctttc ctttggcgcg
    39721 gataaaattt ccatcaactc ccctgcgctg gcagacccaa ccttaattac tcgcctggcg
    39781 gatcgctttg gcgtgcaatg cattgtggtc ggcattgata cctggtacga cggcgaaacc
    39841 ggtaaatatc atgtgaatca atataccggc gatgaaagcc gcacccgcgt cactcagtgg
    39901 gaaacactcg actgggtaca ggaagtgcaa aaacgtggag ccggagaaat cgtcctcaac
    39961 atgatgaatc aggacggcgt gcgtaacggt tacgatctcg aacaactgaa aaaagtgcgt
    40021 gaagtttgcc acgtcccgct aattgcctcc ggtggcgcgg gcaccatgga acacttcctc
    40081 gaagccttcc gcgatgccga cgttgacggc gcgctggctg cttccgtatt ccacaaacaa
    40141 ataatcaata ttggtgaatt aaaagcgtac ctggcaacac agggcgtgga gatcaggata
    40201 tgttaacaga acaacaacgc cgcgaactgg actgggaaaa aaccgacgga ctaatgccgg
    40261 taattgtgca acacgcggta tccggcgaag tgttaatgct gggctatatg aacccggaag
    40321 ccttagacaa aaccatcgaa agcggcaaag tcaccttctt ctcgcgcact aaacagcgac
    40381 tgtggaccaa aggcgaaacg tcgggcaatt tcctcaacgt agtaaatatt gcgccggact
    40441 gcgacaacga cacgctactg gtactggcga atcctatcgg accaacctgc cacaaaggca
    40501 ccagcagctg cttcggcgac accgctcacc aatggctgtt cctgtatcaa ctggaacaac
    40561 tgctcgccga acgcaaatct gccgacccgg aaacctccta taccgccaaa ctgtatgcca
    40621 gcggcaccaa acgcattgcg cagaaagtgg gcgaagaagg cgtagaaacc gcactggcag
    40681 cgacggtaca tgaccgcttt gagctgacca acgaggcatc ggacttgatg tatcacctgc
    40741 tggtgctgtt gcaggatcag gatctggatt taacgacggt aattgagaac ctgcgtaaac
    40801 ggcatcagtg agttgcaggg taagcggatg cgatgttgtt gtcgcatccg gcaaaaaaaa
    40861 agggcaaggt gtcaccaccc tgcccgtttt ctttaaagct gcaaagatta cttcgcgtta
    40921 taattacgca gagcgttacg ccccagcaca atccccgcgc caaccatgcc gcccagcagc
    40981 accgccagaa tcaaggtaat tgcctttttc gggctatcgc gacgaatagg taacgtcggt
    41041 ttcatcacat aacggtaagc atgaatatca agatcatcaa cctttaagtt ttcaatatcc
    41101 aacaggtttt gacgtgtctg atagtagttt ggtgagaaca ccaacggacg ggtcgcctca
    41161 tgcttaatca tcgactccag cgcttcgctc cccaaaagga acaacgtatc ttgtgtcaca
    41221 tcttcagtct gctgaacctg tggctttgcc acctgcgcct gattcgcata ctgcaacgct
    41281 tcctgaatct gacggatacg cagatctttc tgctcctgcg cgactacttc ctgcgttctt
    41341 aaagagtcct gcaagttttt ccgtcccaga gcaatgttgt ctttgagatc cttttctagc
    41401 tcttgattca ctttatcatc aacttgctga atgtattggg ccaacttcat ttgtgctccc
    41461 tctgcagttt gcccaacata agaaacagtc aatggtaatt gctggttctt aacagaaggt
    41521 tcgatggtaa gtttttctgg ttcttcctga ttatccagcg tttctgctaa tgcagagaag
    41581 gcagaactga agcgaccaat taacgtctcc tgtaaatccg atactttcgg tgcagcctga
    41641 ccatagataa cattcatggc attgttatag ccagcaattt gccccacgtc gggctgagtg
    41701 ataattgctg ttgacgtcca tttctccttc gctactgcca aatatccaat agccagggca
    41761 atagccacaa tgacggaaat gatgattgtc atcttgccac gccacaactg cactagtaaa
    41821 tcaatcaaat caatctgttc cgggtcatgg ttttgcccag aaacattatt attttctact
    41881 ctcatcatta ccctaactga cggaaatttc caagtccgca tagtttagcg ataatcctag
    41941 tgaatgctat aggataagtg ataaaaataa tgaattattg gggaggtaac aaaagacaat
    42001 cagggcgtaa atagccctga taacaagatg ttagtcgctg ccaaagagat cgcgggtgta
    42061 taccttatct gccacatctt taagctcttc tgccattcgg ttagagataa tgacgtcggc
    42121 ttgttgtttg aagatggcga gatcacgttc cagccgagag ttgaagaatg agtcttcttt
    42181 catcactggc tcgtagatga tcacttcaac gcctttcgcc ttgatacgct tcataatccc
    42241 ctgaatagaa gacgcacgga agttatctga accgctcttc ataatcagac gataaatacc
    42301 caccacttgc ggtttgcgtg acaaaatggc atcggcaata aaatccttac gcgtgcggtt
    42361 tgcatcgaca attgccgaga tcaggttatt cggcacagac tgatagttcg ccagtaactg
    42421 cttggtatct ttcggcaagc aataaccacc ataaccaaac gacgggttgt tgtaatggtt
    42481 gccaatacgc ggatcgagac aaacgccttc gataatctgg cgagtattca gacctaaact
    42541 ttctgcgtag ctatccagtt cgttaaagta tgccacgcgc atcgctaggt aggtgtttgc
    42601 aaaaagttta atcgcttctg cttcggtcga atcggtgaac agggtcggga tattttgctt
    42661 aatcgcgcct tcctgtaaca acgcagcaaa acgttctgcg cgttctgaac gctcaccgat
    42721 gacaatacgt gaaggatgga gattatcgta aagggcttta ccttcacgga gaaattccgg
    42781 agagaaaata atattttcag tacgatattt cttatgcatc gcttcggtaa aaccaacggg
    42841 aaccgttgat ttgatgacca taaccgcata aggatttatc tcaactacgt ctttaattac
    42901 tgattctaca ctggatgtat tgaaataatt agttttagga tcatagtcgg ttggagtggc
    42961 gatgatgaca taatcagcat cccggtaggc ttcattttta tctaatgtgg cattaaagtg
    43021 tattttatct gattgcaaaa actgctgaat ttccttatca acaataggag atatccgatc
    43081 attcagcata gcaacgcgtg acgataaaat atctaatgcc acaacctcat gattttgtgc
    43141 gattagaagc ccgtttgaca agcctacata gccagtaccg gaaatggtga ttttcatttc
    43201 atgctctcag aattaactta actgtgaatc atgatgtttt tagcatcctg ataagagcta
    43261 aatgttttaa cgctacgtta ttagctggtc aacgctacga aaaacaattc agattttctt
    43321 tataagaata gctgatattt attaagttaa tattaagcag tgaaatttag tctataagat
    43381 aaatggcaaa aaaaaagccc ggtgcaatat acgccgggcc tcaattttac tgttggttaa
    43441 atcagattaa tccagccatt cagtatggaa cacaccttct ttatcaatgc gcttataagt
    43501 atgtgcaccg aaatagtcac gctgtgcctg gatcaggttc gcaggcagaa cagcggcacg
    43561 gtagctatcg taataggcaa ccgcagcggc gaaggtcgga accgggatac cgttctgtac
    43621 tgcataagca acgacatcac gcagcgcctg ctggtagtca tcggcaattt gcttgaagta
    43681 cggagccagc agcaggttag cgatctgcgg attttcggca tatgcatcgg tgattttctg
    43741 caggaactgc gcacggatga tgcagccagc acggaaaatc ttcgcgattt cgccgtagtt
    43801 cagatcccag ttgtattctt cagacgcagc acgcagctga gagaagccct gagcgtaaga
    43861 aacgattttg cccagataca gcgcacggcg aactttttcg atgaactcag ccttgtcgcc
    43921 tgctgactga gcttgcggac cagagagaac tttagatgcg gcaacgcgct gatctttcag
    43981 agaagagata taacgtgcaa acacagactc ggtaatcagc gacagcggtt ctccgagatc
    44041 cagcgcgctc tggctggtcc atttaccggt acctttgtta gccgcttcat ccaggatcac
    44101 atcaaccagg tagttaccgt cttcatcttt tttagtgaag atatctttgg tgatgtcgat
    44161 caggtagctg ctcagttcac cgttattcca ctcggtaaag gtctgtgcca gttcttcgtt
    44221 agagagattc aggccgcctt tcaggagaga ataggcttca gcaatcagtt gcatatcacc
    44281 gtattcaata ccgttgtgaa ccatcttcac ataatggcct gcgccatcag caccgatata
    44341 ggtaacgcac ggttcgccat cttcagcaac ggcggcgatt ttggtcagga tcggcgcaac
    44401 cagttcatag gcttctttct ggccaccagg catgatggac ggccctttca gcgccccctc
    44461 ttctccgccg gaaacaccgg taccgatgaa gttaaagcct tcggcagaaa gctcacggtt
    44521 acgacgaatg gtgtcctgga agaaggtgtt accaccatcg atgatgatgt cgcctttatc
    44581 gagatatggt ttgagcgaat caatagcagc atccgtgcct gcacctgctt tcaccattaa
    44641 caggatgcga cgaggcgttt ccagagattc aacaaactct ttcaccgtat agtaaggaac
    44701 cagtttcttg cctggatttt cggcaatcac ttcttccgtc ttttcacggg aacggttgaa
    44761 aatagagacg gtataaccac ggctttcgat gttgagcgca aggttgcgcc ccatcactgc
    44821 cataccgact acgccgatct gttgctttga cattgtttac tcctgtcagg gggtggtcac
    44881 cgccgagtgt gcggcttaaa tgtggtatag atgttaactc actcatagag atggtgaata
    44941 gcattacctt tgctatacca cactgaatat ctcacctttt ccgtctttta aaacttattt
    45001 atatgaaatt actgaattac ttgttcagta actcaagaat aagttttgtc ttttcttcca
    45061 tcagcggcac atcaccgcga gattccacat tcaaccgcac caccggttcg gtgttggagg
    45121 tgcgcaggtt aaagcgccag tcggcaaagg tcatgctgat gccatcggtg cgatccaccg
    45181 ccagcgcctc gcggctaaaa tgctgttcca cgcggttaat cgcctcaacg ggttgcgcca
    45241 gtttgctgtt gatctcaccg cttgccggaa acgctgccat ccggtcgcgc accagttcgc
    45301 ccagcgtttt tcctttcagg cacaccagtt cggcgaccag cagccacggg atcatgccgc
    45361 tgtcgcagta agcgaaatca cggaagtaat ggtgggcgct catttcacca ccgtagatgg
    45421 cgtcttcctt gcgcatccgt tctttaataa aggcgtgtcc ggttttcgac atcaccggcg
    45481 tgccgcctgc ggcagtcacc acatcaacgg tgttccagga gagacgcgga tcgtggatga
    45541 tcttcgcgcc gggatttttt tcgaggaatg cttctgccag caggccgaca atgtagtagc
    45601 cctcaataaa ctgccctttt tcgtcaaaca ggaagcagcg gtcaaaatcg ccatcaaaag
    45661 caatgcccat atccgcaccg tgtttgatga ccgcattgcg ggtgtcgtcg cggcattccg
    45721 gcagtagcgg gttaggaata ccgttgggga aattgccgtc cggcgtgttg tgcactttga
    45781 ttaattccac gggtgcgcca agggctttaa agcgggcttc gatggcgtcc accaccggac
    45841 ccgctgcgcc attcccggag ttaatcacca gcttgagcgg cgtgaggttt ttgacattga
    45901 tataaccgaa caggtgatca acgtaagcgt cacgcagatt gatttgctga tagcgaccgc
    45961 gtttggtttc atcaacggga ggaaagtcgt tggcttctgc cagacgctgg acatcgcgca
    46021 gtccggtatc accgctgatc gggcgagccc cttcgcgcac cagtttcatg ccgttgtaat
    46081 ccatcgggtt atggctggcg gtaacttcaa tgccgccatc cacgccgaga tggaacgtgg
    46141 cgaaatagat ctcttcggtg ccggacatgc caatatccag cacatcgacg cccgcatcct
    46201 gtaaacctct cgccagcgcc agttttaagg tttcgctggt gaggcggaca tcaccgccta
    46261 acacaatggt tttcggtttg agaaattcgc cataggcgcg accaatgcgc caggcgatat
    46321 cttcattcag ttcttcgcct aatttcccgc gaatatcata ggctttaaag caggttaatt
    46381 ttttcatgtc gttacccttt ttcaggcaat agttggccct gaccgaaacg gccaattttg
    46441 tttttgtcgt tatttacatc tcggtttcgc attcattgcc tgatgcgacg tttacacccg
    46501 tccgtagcga tccgcgaaac gcaccacatc atcctcttcg agataagagc cggagcgcac
    46561 ttcaattaaa tcgagcggaa ttttccccgg gttttccagg caatgcatcg cccccagcgg
    46621 aatataaatg gactcgtttt caccaagcag tttgatatca ccatcaatgg tgacttttgc
    46681 cgttcccgcg acaaccaccc agtgttccgc gcggtgatgg tgcatctgta ccgacaagcc
    46741 ctcgcccggt ttcacggtga tgcgtttcac ctggtagcgg tcgcccgcgt cgatagagtc
    46801 atatttgccc cacgggcgat agacttcgcg atgcacccga tgctcatggc gaccatcggc
    46861 tttgatctgc tcgaccactt ttttcacatc ctgcaccgcg ttacggtcgg caatcagcac
    46921 cgcatctttg gtctgcacca ctaccagatc tttcaccccg acggtggtta cgaggccaga
    46981 ttcggcgtac acatagctgt tttcagtttt gtgattaatc acatcgccgt ggcaaacgtt
    47041 gccctcggcg gtgtgggcgc taatttccca taatgaggac caggagccga catcgctcca
    47101 gcccgcatcc atcggcacca caacggcatc tgccgtacgt tccatgaccg cgtaatccac
    47161 cgactcttcc gggcaggcga gaaacgcttc ttcatccaca cgaataaaat cgagatccgg
    47221 atcgacggcg ctcatcgctt tttcacaggc atcgagaata tccggacgat attttttcag
    47281 ttcttcgaga tagcgtccgg cgcggaacag gaacatgccg ctgttccagt aatattcgcc
    47341 gcttgccaca taggcctggg cggtttccag attcggtttt tcgacaaact gcgccacttc
    47401 aaaggccacc gtatcctgct cacccgcaga cacttcaccg cgacgaatat agccataacc
    47461 ggtttctggt agatccggca caatgccgaa ggtcaccagc ttgcccgctt cggcatatgg
    47521 catggcatta cgcacggcgg cacggaacgc gtcttcatcg gcaatcacat gatccgccgc
    47581 caataccagc attaacgggt cgctctccgg gctatgacgt tttgccgcca gcgccgccag
    47641 cgcaatggca ggtgccgtgt ttcgccctgc cggttcgaga ataatgttct cggtaagttt
    47701 gttcagttga cgcagctgtt ccgcgacaat aaagcggtgc tgctcattgc aaatcaccac
    47761 cgggctttcg cactccacgc cgttcaggcg gcagatggtg gtttgcagca tggtgagatc
    47821 gcctttcagg cataaaaact gcttggggta aagtacgcgg gaaagcggcc ataagcggct
    47881 accggagcca cctgccatca caactggata gagtttcgac tgcgccataa ttatccccga
    47941 atatcattta taaattgacg taacacgttc tctttatcga gtgtgcgttc ggcatattca
    48001 cgtgccaccg tgttgtgttt gggcagcagg agcgcctgac ggatccccgc caccagcgcc
    48061 tcgaccgatt ccggttctac gcaaaccgca atgcccggaa aggtttcgca aagctgcccc
    48121 agttctgtgt gggcctctgc ggtaatcacc gcgttaccgc ctaccgccag aatattggtc
    48181 agtttcgacg gcaatacggc atctgccgcg ccgcgttttt gcaccaccag atggcaatcg
    48241 cccatcttca gcagtgcggg taaagcgtca tacgattgca gcgggaaaaa ttgcatgttg
    48301 cgcagtccac gctgctgcgc cattttttcc agccgcgctt tgccgccgcc ctgcccgaca
    48361 atggcaaaaa tcagcggttc atcgcgcaga cgatcggcag cttcaataac gttttccagc
    48421 ccctgctttt caccaatatt gccggagtaa agaatgattt ttttgttatc cggcaggtca
    48481 agctggttac gaagggcgtc aacatcggca tctgcaacat gctgaaaacg ggcaatttcc
    48541 gaccagttgg ggaagaagat gacgttttcc gccgccacgc ctttttcgat ggctttattc
    48601 atcatcgaac gcgaaatcgt ggagacgtta tcgacgttat gcagtccgct acgttcgaac
    48661 gccgttgcca gctgtgccac tttgctgcct ttgccttttc cggcaaggcc cagccccagc
    48721 atggcgtcca cttcgtaatc ctgaatatgc agcacggtac gcgcaccaga aagtttcgcc
    48781 agcaggcgca ttcccggcgt gcaaaacagc gttggcacca cgccgataat gcgatccggc
    48841 ttccagcgac gttgcgccat cagaggaaag aaactgctga cggcaaaact gccgagatgt
    48901 aacaggcgtt tcagggtgct cggctgtttt ggcacgtaca gcgggcagcg ccacaccgtg
    48961 gcggccccct cttctcgttt gtaccgccag gcggaatagt tctcgcccac ctgccactgc
    49021 gggtagtaag gcggtgcggt aataacccgc acctcatgac cttgtgccgc cagccattcc
    49081 accatctcgc cggtgtattt gccgataccg gttaactccg gcgagtagtt aatgccgtag
    49141 accagtattt tcataatccg ggtactccgg cacgcttctc agcgagaaaa taggcgcggc
    49201 tgttagcgtg aacatcgtta ctggcgagca gcgcgtccgg cgtcagccag cggtaatcgt
    49261 catgctgctc atccggcagt atcagctctt cttcagcgac tctgaagcga aaaccgagca
    49321 ccacatagtg agtggtgaaa tccgtgccgg agaagttatc gtcataaaag tgctgccaga
    49381 caccgtaaaa ctggcctgct gttatcggca gacgcagccc cagttccgcc atcgtcagcc
    49441 gctcgaatgc ggcttccagc gtttcgtctt tctgcacgcg ccctcccggc acaaaccagt
    49501 aaccctgcgc cgggcggttg gttcttttgc caagcagaaa ctcaccgcga ctgttctcga
    49561 caataaagtc gagagagaca agcggggtgg agcgcactac cgtggcaaag tcttcctgac
    49621 gtaaaaacat cattaccccc gaaagcggtc ttgattctca aggaaccact ggtaagtgct
    49681 ggcaagcccc gcttccagtg agatttcgtg ataccagcca agctgatgca ggcgcgtcac
    49741 atccagcagt ttgcgcggcg taccatccgg tttgctggca tcaaaaacca cccgaccttt
    49801 gtaacccacc actttggcga tggtttgcgc cagctcgcgg atagtgcagt caacgcccgt
    49861 gccgacgtta atgtgcgaca gcatcggctg ggtgttctcc agccagactt cgtgcgccag
    49921 ctccatgaca tgaatgctcg ccgccgccat atcatcgacg tgcaggaatt cacgcatcgg
    49981 tgtaccgctg ccccatacca ccacgtccgg tgcattctgt gccgtcgcct cgtggaagcg
    50041 acgcagcaat gcagggatca catgcgaatt actcgggtgg aagttgtcat gcgggccata
    50101 caggttggtc ggcatgactg agcggtaatc gcgtccgtac tggcggttgt atgattcgca
    50161 cagtttgatc ccggcgattt tggcaatggc gtaaggctcg ttggtcggct ccagcgtgcc
    50221 ctgcaataac tcgctttctg ccatcggctg ttttgccagt ttcgggtaga tacaggacga
    50281 tccaagaaac agcagtttgt tcacgtcgtt ctgatgcgcg gcgtgaatga tgttgctctc
    50341 aatcatcatg ttctggtaga tgaaatccgc cggataggtg ttgttggcaa caatgccgcc
    50401 cactttcgcc gccgccagat agacctggtc aatgcgttcg ctggcaaaga aatcatgcac
    50461 cgcgcggctg tccagcaggt tcagctcgtc gcgggtgcgt aataccagtt ccacatcacc
    50521 gcgctgttcg agctgccgcc tgatggcaga accgaccatc ccacgatggc cagcgataaa
    50581 aatgcgttgt ttactcatgc ttatgactcc agcgcgatcg ccacgtcgta gccgtgagat
    50641 ttcagcagag agtgtttttt cgccgcttcg aggtcattag ccaccatttc agacaccatc
    50701 tctctgaggg tgatttccgg tttccagccc agtttttcgt gcgctttggt cgggtcgccg
    50761 agcagcgttt caacttcagc cggacggaag taacgcggat caacggcgat aatcacatca
    50821 cccggtttaa cgcccggcgc gtcatgcccg gtgacggaaa ccacaatgcc cttctcttca
    50881 acacccgtgc cttcaaagcg cagtttgatg cccagctgtg ccgctgccat ttccacgaac
    50941 tgacgcacgg agtactgaac gccggtcgcg ataacgaaat cttccggctg ttcctgttgc
    51001 agcatcatcc actgcatttt tacgtagtct ttggcgtggc cccagtcacg cagggaatcc
    51061 atattgccga ggtacaggca cgactccagc ccctgggcga tgttggcgat tgcgcgggtg
    51121 attttgcggg taacgaaggt ttcgccgcgg cgcggggatt catggttgaa gagaattccg
    51181 ttacaagcgt acatgccgta ggattcgcgg tagttaacgg tgatccagta ggcgtacagt
    51241 ttggcgaccg catacggaga tcgcgggtag aacggcgtgg tttctttctg cggaatttcc
    51301 tgcaccagac catacagttc agaggtggaa gcctgataga aacgagtttt cttttccaga
    51361 ccgaggaagc ggatcgcctc cagcaggcgc agcgtaccca tcgcgtcgac gtcagcggta
    51421 tattctggtg actcaaaaga gaccgcaacg tggctcattg cgcccaggtt gtacacttca
    51481 tccggctgta cttcacgcaa aatgcgcgtc aggttagagg tatcactcag gtcgccataa
    51541 tgcagatgga atttcgggtt gcaggtgtgc ggatcctgat aaatgtgatc cacgcgctcg
    51601 gtgttgaatg acgatgcgcg acgcttaata ccatgcacct cgtaaccttt ttccagcaga
    51661 aactctgcca ggtaagaacc gtcttgtccg gttacaccgg tgatgagagc gacttttgac
    51721 atgtcttatt cctctgtatt tttgaattta ttcagtttca acgcgttcgc gtatcaccac
    51781 tgcgggattc ccccggcaaa ccacatttgc cggaagcgat ttaaaaacac tgcttcgtgc
    51841 acccacgacg gtgccgtcgc cgattgtgac gcctggggca acaaagacat cggttgccag
    51901 ccagcatttc tcgccaatca caataggcgt ggcgttaatg gtgaaatgtt gacttgcatg
    51961 gtcgtggcta ccggtgcata aataactttt ttgcgatatc accgaatgtg cgccaatggt
    52021 tatttcaccg agggtatata aattgacgtc atcgccgacc cacgcgtaat cacctaaggt
    52081 taatttccac ggataggtaa tttttactga cggacgaata actacgtttt ttcctatttt
    52141 tgctccgaat aaacgtaata aaaaagcccg ccagcgatac aatacttgtg gcgaccaggc
    52201 aaatattgtt gcctgtactg cccaccataa ttgcacttta atagcgttgc cgccccggaa
    52261 ccctttcggc accgagaaac cgcttaaatc ttgcattggt tttccttata ttcagacttt
    52321 gttatataag gctttcgtct ttgaggtagt acgttggcgt aaatgccagg ataattcagc
    52381 ccaaaagcca ggcacatgta atatttgtcg ttggactttt ttcgcgtcag cacacaattc
    52441 catattattg gtggtagata ccccacccat ggaaaattca gacaccaggc cattgagttt
    52501 tttaaatgca taaccggctt tatacatttt ggctgccagc gcgtagtcgg aagaaacttt
    52561 atattccagg tcataacgcc attttttcaa gccggataca gggaaaaata tcgcctgatg
    52621 actggcgggc aggctgtgat aaatatacca gcccggtttg gcgctacgtt taattttatg
    52681 cccgtcgcca aaatccagca gcgcatcgcc ggtgatcatc atgttatctt tttgcatttt
    52741 taacttacgg acaaaatttg cggcatcctg atgaaaaata tcgcccgaat tgagaaacaa
    52801 cgcgaacttg ccttgtgcca tcgtaatgcc tttgttcatg gcgtcgtaga taccgttatc
    52861 tggctcgctg acaaagcgta ggttatagat accattgaga ttttccagat actcacgggt
    52921 gccgtcattg gaaccgccat cgacaacaat ccattcgaag ctgatatctt ccgcctgcgc
    52981 cagatgcgcc agcgaggcat gtgttttgac tatcccttcg aggttacgaa acgcgacagt
    53041 gattatgcta agcaacatgt tcttattctt acctcgtaat atttaacgct tttcgcaaaa
    53101 taaatggaca gacgattaaa aatgcatatt ccgggctaaa tatcgaaccg gtaaaaaaca
    53161 gcgataccgg agtaaaaaga tataattgca cgcgaaaatt gcggttatca ccaaaagcgt
    53221 tgatcatcat ttttatcact ttccccatgt accacagcga taaaaacact gcgaaccagg
    53281 aaaaataaat aatcagcaga tacaaaccat tgtctatggt ttttccgaca tccgcaccgt
    53341 taaatattcc gaatgatgcg acgtattcat aaagtgagcc aaatctgact acaccgtcaa
    53401 tatgggtcaa agaataaccg accatcacca gcggaccgac aatacgataa tacgatgacg
    53461 atccttccgt acctaaatcg cccagacggg tggaaatata aggaaacgcg attaccacac
    53521 caaccaggaa tacagccaga gaaatcaatg ccaacggtaa ctttttctta atcgcctctt
    53581 tattcagata ttgaaatgcc cactccagca aataaaacag gataaaagtc ataacccctg
    53641 aaaacgatcc ggataatatt atccctgcga gaatcatagc atcggtttta ggcgttttga
    53701 taccaaactg tttgatgctg agccaaattg agattaatgc cagagcgaaa aatgccggtt
    53761 cgaaataaag tgctgtcgtg cgcttgccgc cgaatttaat gaaattcagt acatagctgt
    53821 tgctgtaaat cagatatttc gaaatcgact ccataatact gctgccaccg gtgaggataa
    53881 tttgcgccat ctccaccgcc gccagcgcca ccaccagccc gatcaccaga taaaagaaac
    53941 gtaatatctt gcgatggttg tgtggcgaaa tcgttttaaa gcgaatactc cacaccatgc
    54001 caataatgat cacaatatag acaaacagca tggttgaagt gacgtattta ctggcatcca
    54061 gcgactgacc aaacagatag ttaaacgccg tgagtcccgc accaatccct aaggcaatca
    54121 tcaatttttt aacgctgatg cgttctaaaa acagcagtaa caagacaggt aaaaaagtga
    54181 cgatggtgat ggggaaactt tcgccaagct gggcgatttt gacgttaacc agcaggtaga
    54241 tcagcggcag cagcaggtag ctacagattc tgatagaagt tgacatactc ctccagcatc
    54301 tgttgtccac tgtaggtggc gcggctgcgt tggctgaact cagccagcgt ggtaccaaat
    54361 atcgcctgcg cgatttccgg tttgcttaac tgcaccagtt gcagcacgtc ttcttcgtcg
    54421 acggttttac cgccggattt ttgcaacact tcccgtgccg cgtcgctatg ggtggcaatc
    54481 accggcacgc caattgataa cgcctcacac aaaatcagcg ggtagttatc gacgcgagaa
    54541 ctgaatacca gcgcatccat ctgattgagc gcgctcatca acttgcgctt gtcagtttca
    54601 aagccgtgat taaccacgtt gccagcggtg aacggcgaga acttaccaaa ggtatgcagt
    54661 tcgattttgt cgcccagcgc catcatctca cgcaccagtt gctggttagt cttgccgtcg
    54721 taacgcaagt catgcgccac caccgcgatt ttcggcttgc cctgggtttc gcgcaccgga
    54781 ggcaagtccg ccagaatcgc ttcggttgcc atatcaatac cattattgat aatccggcaa
    54841 cgccctggac cgtacaggct attgaaagcg tcagccacat gctggctggg ggaaataaac
    54901 tgacagccca gcgccagcat ctcacggaat aactggcgtt tgcccgccac cagctggtgt
    54961 gcgcgatcaa tcttcaccgg cggatagtta tttaaggtcg ggcatttctg gcagcccgtt
    55021 ttccagcctt cgcaaccgtc ggtaaaggcg cagcgtccgg taacactcca gtggtcgtgc
    55081 agcgtccaga ccagagtgac gtccggttta tggtttttca ctttttcgca aaagcgcacc
    55141 acgctcttaa gatttagcca gtagctgtgc agcacatgaa aatgcaggac caccggaccc
    55201 ggtgtgcgag taatggtgcg atataactca ttgaaattgc caaacagatc gcgattaaac
    55261 agacgaaaca gggcaatatt cgccatcgcg gtcatccgcg gcgtatgttt gatgacctgc
    55321 ggatagttct gatggctgac gctctctttg ccgcctttgc cgtaaccgta gacaaaatgt
    55381 gacgccagcc cctgttgcag cgcacgctgg tggagatcta acgccacacc tgctgccccg
    55441 ccttccgcca gtcgcacatt aaattgcaga atattcattt aattaccttc actcgcgctt
    55501 tttctcccac caccagcgcg ttgtccggga cagagtcgag caccacgctg cccgcaccca
    55561 cggtgacgtt gttaccaagc gtgatatcac caagaataat gacgttggca ccgagttcga
    55621 cgccgttgcc aatgtgtgga catgccatgt tatcggcacc acgattgccg atagtgacgc
    55681 cgtggcgaat ggtgaaatca tcccccgcta ccacgttttt attgatcact acggcgtaac
    55741 cgtgatggat agtaaagcgg cggccaatgg tcgcggcggc ctggatttca taaccgaaaa
    55801 agcattcggt gataatgcga tacagcacca gcagcggggc cgcccacaga ttgttgagga
    55861 cgttcttttt gcgccacacc gaacaaaaat gagcaacacg ataggcaaga accatgcagc
    55921 acgggcgtaa actccagctg ttggcacgca gatcttccag catctttagc gcccccgaat
    55981 accatcagcc agacgtttgc cgttacgcac cgacagcagc gtcagcaaag tgcgccaggt
    56041 catacgttta ttgcggatct ggtagagggt aaacagctga tattttttgc ttgcacggtc
    56101 aaatttatct ttgtgctttc ggtaaaagtg gaagtacccg gagaattttt tcggtgacga
    56161 ggtgatctgc atttcaccgt gattgatatg caggatctgc gtcgcttctt ccactttcca
    56221 cggttcgccg tactccacca ccatccgcag gaagatgtcg taatcctgtg ctgctttcag
    56281 ttcggtatcg aacaaacact ctttgaaacg ccatgcccaa gtaaagacct gattgccaat
    56341 gatattgcgt ttatagaaca ggcggcgtga gtacggcgat ttgggataca gtggtaagct
    56401 tgccggttgg gaataaactt ctccctggca tacgtagtcg ttagcgtaca aaaaggcgtg
    56461 agtgaccagt tgctgtttat gggcgaggaa gacgctcaga cggttgggtg tccattcgtc
    56521 atcgtcatcg atcccggtaa tgtattcccc ttgcgccagc ataatcgcct ggttacgtac
    56581 cgcgcacgcc ccgctgttaa tgtcgttgtg aatgtaggtg atacgcggat cgttgagggc
    56641 agtgacgtac tgttgcagtt gttcccagga ggtggagcaa tcatccacga tgatcatctc
    56701 ccagttgctg tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg
    56761 gttccaggtc ggcatataga ttgaaatcag cggattctct ttcataggtt gttctcccgg
    56821 cttatgagct ggtttgcttg ccggatgcgg cgtgagcgcc ttatccggcc tacggggcgg
    56881 tgcgaatgca ggcgtcgtta tttcgcatcc gacttatatt cgtattcgta atagccataa
    56941 tcctgatacg cgctggcgcg gcggaagatg gagttcagaa tcaccccttt caccggaata
    57001 ccgttttgct caaagcggct cagactggtt tccacttctt tcaatgtgtt gaccgcatag
    57061 cgcgccacca ttaacgtggt tccagcgtga cgcccgacaa ttgctgcatc ggtcactgcc
    57121 agaatcggcg gcgtatcaat caacaccagg tcgtagtttt tactcgccca gctcaccaat
    57181 tcggcaaagc gttcgctcat caacagttca gaaggatttg gcggcacctg accgcgcggg
    57241 atcaggtcaa atttggcaat agaggtcggt ttagctgcgg tagtaatatc gccctggcca
    57301 atcagaattt ccgacaggcc attgacgtta ttggtaccca acagctcgtg ggtgtagcct
    57361 ttgcgcatat cgcagtcgat caacaacacc cgtttattgg tctgactgat caccgccgcc
    57421 aggttggcgc agacaaaggt tttaccgatt gacgggctca cccctgtcat catcaacaca
    57481 ttgttctgcg cctgcatcat cgcgaagtgc agactggtac gcagactgcg gatggcttca
    57541 atcgccagat cggttggatt ccccaccgcc agtagctggc tctgtttata gcgtttaatc
    57601 cctttgatgg ttttgacgct atcgcgcgct ttctgccatt ccgacagcgg gatgctggca
    57661 tagacgctga taccatgttc ttccagcacc tgcgggcttt cgatgccgcg attaaacaac
    57721 gagcgcagca gcacacccac gatagagagc atcaggccga ggataattgc gccgaggata
    57781 atcagtcctt tcttcggttt cagcacgcca ggctgggtga ttgccgggtc aacaatgcgc
    57841 acatcgccga cggtgctggc ttcggtgatt ttcagctcct gctctttatt cagcagttgc
    57901 atatagacct gctgaccaga ctcgacatcg cgggtcaggc gcacaatctc ctgctgggtt
    57961 ttcggcatcg ccgttacgcg accgttaagt ttggctttct cttcttccag cgcctgacgt
    58021 ttctccagca gcgtgcggta cgccgggtga actttggtgt acagcttgga gatttccgcc
    58081 tctttaaagg tcagttcgtt caactgcgcg tcgatgttca ccatcgaatc gagcaccgct
    58141 ttcgcttcca gcggcagatc aacagaatct ttatcctgac ggaaggcatt cagtttgttt
    58201 tcggcaacat cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa agcgaggctt
    58261 ttcgacgctt ccgccgattt gcgctcaata ttttgttcct gatagttacg ggcgatgctg
    58321 ttaagaatgt cgcggatctg ttcgcgatct tcaccggtat aagtcaggct cagtacgcct
    58381 gcgtctttgc cgttctccgt taccgtcagg ctgttttgca gttggttgat catccccaac
    58441 gtggagtatt tggtgacggt aaactcactg cccgggcggg cgtgaatggc ttcaaccatc
    58501 agcgtgacgc cttctttttt cagcatctgg cccgcttgcc cacgggcgct aaagccgcca
    58561 tcactgctca gggtgtagtt tttattgtcc agcacattaa gcgtaaacac ctggcccgcc
    58621 atctctttcg ggcggttaaa ggtagtcact ttcaccgtct cgttctggcg tcccatcagt
    58681 cgctcccagc ccgccccgaa aatcgggaac gtgtttttgc tcactgcaat atcgaggtcg
    58741 agatcgtcca ccgttttacc aagcaccaga cgcgaacgaa tcaactggat ctcggcgtcc
    58801 gatgcaggcg gtttgttggc taatgccgaa ccaatgtcct gcactaacga attgccgctg
    58861 ttttgctcga tttgtaccag tgcgtcggca ctataaatcg gcgttgcgaa gaaggtgtaa
    58921 accacggcac agagggcaaa tacagcggtg atgccaatca cccaccagcg cgcttcaatg
    58981 acggtgccga ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg agcggcatgt
    59041 tgttttactt tttctgtcat tcttatacct gctctgcgtt caatgcctgc gcccactggc
    59101 gggcagaccg ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt ttgcgatacg
    59161 gatcggggat ttcacattcg ttatcccagt gaccaaacag catcactttg ccgcgcatct
    59221 caggtgccat ctcgcataag cgttcgatat ggcgcttttc catggtcaaa atcaggtcat
    59281 agttgcgaca caggcggcgg ctgatttgtc gtgcacagtg gccttccaga gacagttgat
    59341 gttctgcggc gacgctgata gcggtaggat cagcgccctt accgaccagc gcgccgagtc
    59401 cagcggactc cactttcagt tccgggtgat aacgttgcag taagcgttcc gccgtcgggg
    59461 aacggcaaat attgccgaca cagacaacta atatgttgtt aaacatgacg attaccagtt
    59521 atgaatgtcg ctggctgtat ccgtcatgta acggacaccg ctaatagttg gcaacaactg
    59581 attgatcaga cggttccagc gggaaaccgg ggcggtggtg acatacacca cgtcataagg
    59641 ttgcaggcgg aattctgtcg ccatcaccag cgacgtggca tcggacatat ccagctggta
    59701 gatattggca atcttgccat tacgcccgcc ctcgcctttc agcggacgaa tgacaaagat
    59761 gccgctggcg ttggaggtgg tcatgtcgat accttcagca ttacccaggg cttcagtcag
    59821 ggtcatgccg ctaaagtcca ttttcagggt gctctgtttc ttcacttcac ccatcacaaa
    59881 tactttcaga tcatcattac gcggcacgta gagaatatcg ccgggataaa gcagtcggtt
    59941 ctgattgagg tcgccgtttt gcatcagcgc ctgcaaagaa atgcgttctt cacgaccatt
    60001 gtgtgttagc actacgttgc gccagtcagc ggtgtcggtc aggccacctg cggcgttgat
    60061 ggcgtcgaga atagtcagtg gcacgttggt gatcgcctgt tgaccggatt tattcacctg
    60121 accggagata taggcctttt gtgagcggaa ggcggcgata ttaacgtcca cctgcgggtc
    60181 agcgatgtac gtcgctaagc gcccggtaat atcactgcgg atttcagcga gcgttttccc
    60241 gactacgtga accttgccga tatacgggta aaacatagtg ccgtcaggct gtacccagtt
    60301 gccggtgtcg ctggagctgc ggtactgacc ggctggcgtg gtgagttccg ggtgatccca
    60361 gacggtgaca ttcagaacgt cgcccggccc gacgcgatac tggtaattcg cgatctcgct
    60421 ttccagcgtc atattggggc gcgctacatt cgggcgtggg cgtaattggt caatcaggcg
    60481 cggggtcagc ggataaacat tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt
    60541 gatgacgtct ttgcccatcg tcgacatatt gctgcccgga agtactgtgc aaccgcttat
    60601 caaggttact gacaccaata atggcatcaa tttcattttg gatttcatca ttgtttattt
    60661 atcactttgg cagagtaatt atcctgtgca ctattaatag caatatcgcc atgcacattt
    60721 accttgcagt taattgaata aaaatttaac tggcatcagt cctaaaaaaa ttgatttcat
    60781 ccgcaggcta ttgacagaat aattcagact ggtctttcag gcatccagac acgctaccgc
    60841 ccctggcttt ttagccacca atacactgat ttagcttaat ttttcaaacc ctctctgcat
    60901 acaatcgttg atgagaaagg gttattgcgg aaattaactt ccgaatataa ggtgacatta
    60961 tggtaattga atattggctc tccaataatg caggaggaag tgttacagct aacggaatag
    61021 caggcaaggt aacaattcgg caattggcta tttttaagaa ttatattaag tgtcattcaa
    61081 tatggttttt aggagtttct ttaggttgac aatatttaat attgcgcctc cacatgcgat
    61141 atttcttaaa taatgtttta ttattaccac ttttaattca gggataatgt gaggttatta
    61201 ccctcaaata atatgagata aatagtgctg caacattgca tttttgcccc gatttatcca
    61261 tgatcgaatt gtgacatttg tcatacaacg aataggtttt gtacttacta tggaatggat
    61321 tgccgatccg tctatctggg ccgggttaat cacgctgatt gtgatcgaac tggtcctcgg
    61381 cattgataac ctggtcttta ttgccatcct cgccgaaaaa ctaccgccga ggcagcgcga
    61441 ccgcgcacgg gttaccgggc tgctgctggc gatgttaatg cgcctgttac tgctggcgtc
    61501 aatctcctgg ctggtcaccc tgactcaacc gctgttcagc ttccgctcgt ttacctttag
    61561 cgcccgcgac ttaatcatgc tgtttggtgg tttcttcctg ttgttcaaag ccacgatgga
    61621 gctgaacgaa cggctggaag ggaaagacag caataatccc acacaacgca aaggtgcgaa
    61681 gttctggggc gtggtgacgc aaattgtggt actggacgcc attttctcac tcgactcagt
    61741 gattaccgcc gtcgggatgg tcgaccattt actggtcatg atggccgccg tggttatcgc
    61801 tatcagcctg atgttgatgg ccagcaagcc gttaacgcaa tttgttaaca gtcacccgac
    61861 gatcgttatt ctctgcttaa gcttcctgtt gatgattggc tttagcctgg tggcagaagg
    61921 tttcggcttc gtcattccga aaggctacct gtacgctgcc attggtttct cggtgatgat
    61981 cgaggcgctc aatcagttgg ctatctttaa ccggcgacgt tttctttccg ctaaccagac
    62041 gctgcgccag cggactaccg aagcggtaat gcgcctgctt agcgggcaaa aagaagatgc
    62101 ggaactggac gccgaaaccg cgtccatgct ggtggatcat ggtaaccagc agatctttaa
    62161 tccgcaggaa cggcggatga ttgagcgggt acttaatctt aaccagcgta ccgtcagcag
    62221 cattatgacg tcgcgccacg atattgagca tatcgatctc aacgcgccgg aagaagaaat
    62281 ccgccagttg ctggagcgaa atcagcatac gcggctggtg gttaccgacg gtgatgacgc
    62341 agaagatttg ctcggtgttg ttcacgttat cgacctgcta caacagtctc tgcgcggcga
    62401 accgctcaac ctgcgggtgt tgattcgcca gccgctggtg ttcccggaaa ccttgccgtt
    62461 gttacctgcc ctggagcagt tccgtaatgc ccgcacgcac tttgcttttg tggtggatga
    62521 gtttggctcg gtggaaggga ttgtgacatt aagtgacgtc actgaaacca ttgtcggtaa
    62581 cttaccgaac gaagtggaag agatcgacgc ccgccatgat attcagaaga atgcggacgg
    62641 ttcctggacg gcgaatggtc atatgccgct ggaagatctg gtgcaatatg tgccgctgcc
    62701 gctggatgaa aaacgtgaat atcacaccat tgccgggctg ttgatggaat atttacagcg
    62761 tattccaaaa cctggcgaag aagttcaggt gggggattat ttgcttaaaa cgttgcaggt
    62821 ggaaagccat cgcgtgcaga aggtgcagat tattccgctg cataaggatg gcgagttgga
    62881 gtacgaggtg tgatggtatc ggtcatttta cccaatagcc gaaaggaata agatgaggga
    62941 accgcgccag cggcgattta ccgccgggag cccgggttgc cagggtggtg gcgattgagc
    63001 caccctggca cgttcaccgg cttgagcgtt ccatagtagc gaggaacatc aagtgaacgg
    63061 aacagccttc gatcttacat attcagaata taatgatgcc gccacgaacg cctgatgcga
    63121 cgctttacgc gtcttatcag gcctacagga ctggtcacct ggtaacctac atcttctcca
    63181 gcaacttctt cacatccttg ccattgcggg aatctttatt ccgctcggcc cagtcattca
    63241 ggcgacgttt cgcttcgtcc tgtagatgtt tgcgcagtaa ctgatccact tgcaggctgt
    63301 aattgagttg ctgccagttg ccataaaccc gcagcggtac tggcgtttct ttcaggaaat
    63361 caatcagttt actttccccg ttccagccac ccacgacccg aatatcaaac tgggtatcgc
    63421 aggtttgatc tgccagattc aacatgcctt cccctgtcag cgccagcaca ggcgattgac
    63481 cttgcatgtc gtttaacgtc acgacgccat ccttcaacgt caaatcggtg gtaaagcggt
    63541 caagccgcgt cacgttatca aagttttcag cggccttcac atcaccgcca ttacgctcta
    63601 ccgcttgctg aatcatctgc tggaagttca tcccttccat gcgcgtgtcg gtcatttcga
    63661 catgtgcctg tccttgccag ttgtggcgga atgcgtcggc atctatgtca gcaccggaga
    63721 aatcaccagc cagtgacatt tttccggtca acgaaatcgg atagttaaac gccttcagaa
    63781 tggtaccaat ctcaacgttt tccagccgtg gctggaagtt tatccgcgga tttattgatg
    63841 tcgcgtccag cgtgcccggc agtgaaacct gtccaccgtt aagtttgccc tgcagttgag
    63901 taatttccag caaacccgac ttgttggtca tttgcgtggc aacatctgta aaattcattc
    63961 cgcgccagcg cacgttactg gcctgcaaca aaatatcagc cgtaaagcct tgcagtccct
    64021 gataggccgg ttcatcaata cgcgaagaaa tgaccgggcg cggcaacgtt gattggctct
    64081 gcccctgctg cgcggcaccg ttttcaccat tcgcggtttc attaagcggg atgaggttgt
    64141 caagattcag ttgcgggaat tgcagtctca gctgccattc cggtttctct gtcagcgtga
    64201 cctgtgcttg cccgctcagc gtactgtcat tggcggtcaa gctgatttgg ttaaatgaaa
    64261 ggcgtttatg tgactcctgc cactgggcct ggaaactccc ctgcccctga ataccttgtt
    64321 ttggtaaatc ggcaccctgc aactgccagt taatttgttc aatagccgcc gttaaatcat
    64381 gcggataatc agaagcatcc accgtaccgt taagggatat tgtgagatcg cgctgatcgc
    64441 gattaacccg cccggagaac tcaaatgagc cacgatgttg gggatcttgt tccatttgca
    64501 ggcggatatt gcggattgtc acctgctcgt catcttcatg ctggaacacc agcacgctgt
    64561 ccgccacctt aagactggat atatcaaacg accatccgcg atcgtctgac agatccggca
    64621 aggtattgtc gcgcggtgca accggagcgt cttcactgcg caccgcttcc gtctgcggcg
    64681 tcagttggat cactgcccct tttagcatca cctgcttaac gctcagttga tgactcagta
    64741 gtggtaaaag cgccacgtcc agacgcatgt tgtcggcgcg aaccagtggc tggcttgcgc
    64801 cctgggcggt gagagacatt cgcccggaga ggatactaag ctgcggccag acgtgccaac
    64861 gcagtggccc gtcgagctgc aattgataac cgctacgtgc agcaacttgc ttgaccatat
    64921 agtcgcggaa atcattcgga ttcaccagca acactaacgc agataacccg gccaccagca
    64981 cgaccaggag tatcatcagc gtcgtcagaa atcgtctcat ggtatcctca atgggcctga
    65041 attagtcttt atcgattcgg ctggctaccg cgccctgctg gttgcgatat ttcgcatctt
    65101 cacggcggtt gtaaggtcgc gccgccgggc cggaaagcgg ctcaaagctc agcgcaccaa
    65161 ttaacatgcc cggacgcagc gccagcggca gcttaccgga gttgtagaac tccagcacaa
    65221 tgcaaccaga ccagcccgga tcgatacggt gcgcggtgac gtgcaccatc agccccagac
    65281 gcgccaaaga ggaacgcccg tccagccagc ccaccagatc ggctggcagc gtcaccgact
    65341 ccagcgtcac cgccagcgcc agctctcctg ggtgaagata gaacgcctcg ccctcgtcga
    65401 gaacgatctc atcgctcatc acgcggtcaa gcgcggcgct cacttcatct ttgggaccgc
    65461 tcagatcgat aaacgctgcc gtgtgaccac ggaaggtacg aaatttattg cccaggcgta
    65521 catccaccgt cgcgccgtta atacgctcca ctggcggacg tgggttgatc gacaaacggc
    65581 cttcatcaag ccaggcttca atatctcggt cacacagacg catggcattt tctcctttcg
    65641 cgcatcactc ccttaacgcc aattacgtca agggcatacc aggttatcac tgaacggtac
    65701 acaatttatc aagcttattc aaagaactga cttattttcg ctttcaatat atcgatcgcg
    65761 atgcggtttt tcccgccgcg cggcacgata atgtccgcat attgtttaga aggctcaatg
    65821 aattgcagga acatcgggcg cacggttttt tgatattgcg ccatcacgga atccattgaa
    65881 cgcccacgct cgttaacgtc acgcttgatg cggcgcatca ggcagatatc cagcggggta
    65941 tcaacgaaaa tggagaagtt aagttcgtca cgcaaacgcg catccgtcag caacaaaatg
    66001 ccttcgagaa tgatgacctt cttcggctca accgtcaccg tttctttcat acgcgtatgt
    66061 tcaacatagc tgtaaaccgg caggtcaatt gccgagccgc gtttcaacgc ttgtaaatgc
    66121 tcaagcagca ggctgtgatc catcgcgctg ggatggtcgt agttggtctt aacgcgttct
    66181 tccatcgaca gatggctttg atctttgtaa tagcagtctt cgggaattac gccgatgtgt
    66241 tcatcaccga cttgctcacg taattcacga taaagggtac tggcaataag actcttgccg
    66301 gaagccgatg cgccagcgat accgataatg acgcactgat gagactgatc agtcatatat
    66361 ttagcgacct gattaacctg gatgttagga agggggcgac gaagcgccaa acgcggcaat
    66421 tatagggatt tcatccgtct gataccagtc gaatagcgtt gccgcgcgct cagagttaat
    66481 tgttgacaaa gaattcccgg tggcaaatta cgttgatcag ttttatgtaa ggtaaaaaat
    66541 gttatacgca gttgcgcaaa ttatccgcct ttacgtcact ttatgagcaa ttcgcatata
    66601 aaatgtaaaa cttttgtact agcataaaca cagaaacgaa tactggcgac ctggtcttgc
    66661 ggataaagcg gtaatgagca aacaatcaca gcatgtatta attgccctgc cccacccgct
    66721 gcttcacctg gtcagtttag gtttagtctc gtttatcttt acccttttct cgcttgagct
    66781 ttcgcagttt ggcacccaac tcgccccact gtggttcccg acgtccatca tgatggtggc
    66841 gttttatcgc catgccgggc gcatgtggcc gggaattgcg ctgagctgtt cgctgggaaa
    66901 tatcgccgca tccatcctgc ttttttccac cagctcgctg aacatgacct ggacgaccat
    66961 caatattgtt gaagccgtgg tcggggcagt gctgctacgt aaattgctgc cgtggtataa
    67021 ccccttgcaa aatctggctg actggctgcg tctggcactc ggcagcgcca ttgttccgcc
    67081 tctgttgggg ggtattctgg ttgtcctgct gacgcccgga gacgatcctc tcagggcatt
    67141 tttgatatgg gtactgtcag aatccatcgg cgctctggca ctggtgccgc tgggattgtt
    67201 atttaaacca cactatctgc tgcgccatcg caacccacgg ttgctttttg agtcgctgct
    67261 cacgttagcc atcacactga cgttaagctg gctttcgatg ctgtatctgc cgtggccttt
    67321 tactttcatt attgtgctgt tgatgtggag cgccgtgcgc ctgccacgaa tggaagcctt
    67381 tttgatcttc cttaccacgg tgatgatggt gtcgctgatg atggccgcgg atccctccct
    67441 gcttgctacg ccgcgtacgt acctgatgag ccatatgccg tggctaccgt ttttgctgat
    67501 cctgctgccc gccaacatca tgaccatggt gatgtatgcc tttcgtgcgg aacgcaaaca
    67561 catttccgaa agcgaaaccc gctttcggaa cgcgatggaa tattccgcta tcggtatggc
    67621 gttagtgggc accgagggac aatggctgca aaccaacaaa gcgctctgcc agtttctcgg
    67681 gtacagtcag gaagagctgc gcggactcac ctttcagcaa ctgacctggc cggaggatct
    67741 caataaagat ctccaacagg ttgaaaagct gataagcggt gaaataaaca cctattcaat
    67801 ggaaaaacgc tactacaacc gcaatggcga tgttgtctgg gcgttgcttg ccgtctcact
    67861 ggtgcgccac acggatggca cgccgctcta ttttatcgct cagattgaag acattaacga
    67921 gctaaaacgc accgaacagg tcaatcagca actgatggag cgcatcacgc tggctaacga
    67981 agcgggcggg attggcatct gggagtggga gttgaagccg aatgttttta gctgggataa
    68041 gcggatgttc gagctgtatg aaattcctcc acatatcaaa ccgaactggc aggtgtggta
    68101 cgagtgcgtg ctgccggaag atcgccagca tgccgaaaaa gtgattcgtg attcgttgca
    68161 atcacgctcg ccctttaaac tggaatttcg cattaccgta aaagacggta ttcgccatat
    68221 ccgcgccctc gccaaccggg tactgaataa agaaggcgaa gtcgaacgtc tcctcggcat
    68281 caatatggat atgaccgaag tgaaacagct taacgaggca ttgtttcagg aaaaagagcg
    68341 cctgcacatt acgcttgatt ccatcggtga agccgtggtc tgtattgata tggcgatgaa
    68401 aattaccttt atgaatccag tggcggagaa gatgagcggc tggacgcagg aagaagcgtt
    68461 aggtgttccg ctcctgacgg tgttgcatat tacttttggc gacaacggac cattaatgga
    68521 gaacatttac agtgccgaca cctcacgttc cgcaatcgaa caagatgtgg tgttgcactg
    68581 tcggagcggc ggcagttacg acgtgcatta cagtattacg ccgttaagta ctctggacgg
    68641 cagcaatatt ggttcggttc tggtgattca ggacgtcacc gaatcacgca aaatgctgcg
    68701 ccagctgagc tacagcgcct cccatgatgc actgacgcat ctcgccaacc gcgccagttt
    68761 tgagaaacaa ctgcgtatcc tgctgcaaac ggtaaacagt acgcatcagc gacatgccct
    68821 ggtgtttatc gatcttgatc gctttaaagc ggtgaatgac agcgccgggc atgcggcggg
    68881 cgacgctttg ctgcgcgaac tggcgtcgtt gatgctgagt atgctgcgct ccagtgacgt
    68941 gctggcgcgg ctcggcggtg atgaatttgg tctgctgctg ccagactgca atgttgaaag
    69001 cgctcgtttt atcgctacac gtattatcag tgctgtgaat gactatcact ttatatggga
    69061 aggccgggta catcgggtag gtgccagcgc cgggattacc ttgatcgatg acaacaatca
    69121 tcaggcggca gaagtgatgt cgcaggctga tatcgcctgt tatgcctcca aaaatggtgg
    69181 acggggccgg gtgacggttt acgaaccaca gcaagctgcc gcacatagcg aacgggcggc
    69241 gatgtcgctt gatgaacagt ggcggatgat taaagagaat cagttgatga tgatcgccca
    69301 cggtgtcgct tcgccgcgga tcccggaagc gcgtaatttg tggctgattt cgcttaagct
    69361 ctggagttgc gaaggcgaaa ttattgatga acaaacattt cgtcgtagct tcagcgatcc
    69421 ggcacttagc catgctcttg accgacgggt attccacgaa tttttccagc aggccgccaa
    69481 agcggttgcc agtaaaggca taagcatcgc cctccccctt tccgttgccg gtttgagtag
    69541 cgccacgctg gtgaatgatc tgcttgagca gctggaaaat agccctctac caccacggtt
    69601 attacatctg attattccgg ctgaagcgat tttagatcac gcagaaagcg tgcaaaaact
    69661 gcagctggcg ggatgtcgga tagtgctcag ccaggtgggc cgcgatctgc aaatcttcaa
    69721 ctcgctgaaa gcgaatatgg cagattacct gctacttgat ggtgagttat gcgccaacgt
    69781 gcagggtaat ttgatggatg agatgctgat tacgattatt caggggcacg ctcagcgact
    69841 cgggatgaaa accatcgccg ggccagtcgt tttaccctta gtgatggata cgctttctgg
    69901 catcggcgtc gatctgattt atggtgatgt gattgccgat gcccaaccgc tggatttgct
    69961 ggtgaatagc agttatttcg cgattaactg agagagcgtt tgcccattgc ctgatgcgac
    70021 gctaacgcgt cttatcatgc ctacaaatcg ctcattcccc agaccggata aggcgctcgc
    70081 accgcatccg gcgaccaacg tcatgcttcg tctggttgcc agccttccgt ataccagata
    70141 tgcaacagcg cataagaacg ccagggcttc cagcgctcgg cataacggcg gatttgtgcc
    70201 ggtgtcattc ccggaaatcg ccgtttaatc agataatcat ccggcagaaa aacatctttc
    70261 gcctgccagc cacgcaaagc aaaataattc gccgtccagc gcccgatacc cggaaaagtt
    70321 tgcagcgttt tcatcgcctg ctccacatcg cccggaattg tcattggtaa gctgccctcc
    70381 agcgccgcat ttgccagatg aatcagtgcc tctgcccgtt tcaacggcat gcctaacgct
    70441 tttaatgcct gcgggtcggc tgctgccagc cgctgaggcg tggggaagca gatatactcc
    70501 ggaaaatcat ccagccgttc gccataaagc tgtaccactt tggcagtcaa ttttgccgcc
    70561 atcgccacgc tcaccagttg gcctaaaatc gcccgcacac cctgctcaaa agcatcaata
    70621 cagccgggta aacgcaatcc gggccgcccc gcgcctaact ttcccaacgc accgttaaca
    70681 atctgcgggt tacattgcag atcaagcagg cggctcattt tcgccagaca ctctgcggca
    70741 acaggttcta aacctgcact taaatttatg tgcagagtat ggcgggctat atccggaata
    70801 gcagtcacta cgccgcgata ttcgcccacc gccagactac gggcataata attgtccgcg
    70861 accgtttcca caccgctcac cgcacgggcg gcgagaaatc ccaacatcca cgaccagtca
    70921 tacggcggct gccagttcag ggtatacatc gcatctcctt gttaatccgc tttttccggt
    70981 tgtcgcgaca gcaacatttc gctaaagtca cgccccttct tcaccggcat ggggattaat
    71041 tcgtgtttat tggttttgat tacggtacag caaactgttc agtggcggtc atgcgtgacg
    71101 gtaaaccgca attactgaaa atggaaaacg acagcacgct gctgccttca atgctttgcg
    71161 cgccaacgcg tgaatcggta agcgaatggc tgtaccgcca tcatgatgtt ccggcagacg
    71221 acgatgaaac gcaggcgctg ttacgtcggg cgattcgtta taaccgcgaa gaagatatcg
    71281 atgttacggc gaaaagcgtg cagttcggtc tttcctcact ggcacagtac attgatgatc
    71341 cagaagaagt gtggtttgtg aaatcaccaa aatcgttcct cggtgccagc ggcttaaaac
    71401 cgcagcaggt agcgctgttt gaggatctgg tctgcgcaat gatgttgcac attcgccagc
    71461 aggcgcaggc gcagctgcca gaagcgatta ctcaggcggt gattggtcgc ccgatcaact
    71521 tccaggggct gggcggcgat gaagcaaacg cccaggcgca agagattctg gaacgcgcgg
    71581 cgaagcgtgc cggattcagg gacgtggtat tccagtacga gccgatcgcg gctgggctgg
    71641 attacgaagc caccttgcag gaagaaaaac gggtgctggt ggtggatatc ggcggtggta
    71701 cgactgactg ttcattgctg ctgatggggc cgcagtggcg ttcgcgtctc gatcgtgaag
    71761 ccagtctgct gggtcacagt ggttgtcgta ttggcggtaa cgatctggat atcgccctgg
    71821 cgtttaaaaa cctgatgcca ctgctgggta tgggtggcga aaccgaaaaa ggcatcgccc
    71881 tgccgatcct gccgtggtgg aatgcggttg ccatcaacga cgtacctgcg cagagtgatt
    71941 tctacagtag tgccaacggt cgtctgctta acgatctggt acgcgatgcc cgcgaaccgg
    72001 aaaaagtggc cctgttacag aaagtctggc gtcagcgttt aagctatcgc ctggtgcgta
    72061 gcgcagaaga gtgcaaaatt gctctttcaa gcgtagcaga aacccgcgcc tcactgccgt
    72121 ttatcagcaa tgaactggct acgctgatta gccagcgagg gctggaaagc gccctcagcc
    72181 agccactggc gcggattctg gaacaggtgc aactggccct ggataacgcc caggaaaaac
    72241 cggacgttat ctacctgacc ggcggtagcg cccgttctcc gcagattaaa aaagcgctgg
    72301 cagaacagtt gccgggcatt ccgattgcag gcggcgatga ctttggctcc gtcaccgccg
    72361 ggctggcacg ctgggcggaa gtggtgtttc gttaattgaa aaatggctca gcagcggctt
    72421 aaatctgcct gcgcctgagc cagtttttct gctgcctgac gtaacggttc catcaacggc
    72481 atggtccgtg ctggagcatg aaggcggaat cgctgcaact ctgtcgcgcc gactgtcaac
    72541 gccgattcca gccgatgtcg tttggcggtc attttcgcca ttaccgcttg tctgtctgct
    72601 ggcgttatcg cttcattcgg cctgaacaca aaacggcgtt cacagttcgc tttccagtcg
    72661 ataaccgcct gggtcagatg gtcgccaaac cctttcactt gcttaacgcc acgacgggta
    72721 acatctgcgg ctgtttcaat accaaaagac cgtaacgccg ctttacgcgc agggccaacg
    72781 ccgggaatag aggcaacatc aataaaaaat ccctccagaa acttctgctt ctgccgttcc
    72841 cttgcagtgt catgaagtgc tgccagagcg cgtttctctt cttccggtaa cccgagaatt
    72901 tcgtccttca ttttttcgag catcgtccgt ttggcgataa aaccttccag gccgcctacc
    72961 tgctggatct ggcggactaa atggtcatag tccattttcg cacgattaaa tgcctcacgg
    73021 cgttgctgaa cttctgcttt gtacgctttg cttgtcaaac tgccaataat ccagatagcc
    73081 gccagcacag gaaccaaaat aatataacgc ggttctgcct gaaggccgca aagcagcagc
    73141 gataatgctg acagtgcgat ctcaagcaga atgatgtatt cgcgccgtaa caggcctaaa
    73201 ggaagcggcc tgccagtcgg ttggaaatga tcgggtaatg gcagttgcaa tgctggcggt
    73261 gctactgacg ccatcaccat cgcccagact tttgccagca caaaatcccc gccggtggta
    73321 atgacctctt cgccgagatc aataaaatag ataacacctt gattatccag cgcacaccat
    73381 gggcagtcag ccaaatgacc gggataaaca tgcattgccg aaacggtaca tttctttaac
    73441 tgttggcgta gagaatccag tgctgatacc cacgctttag ccgtcggacg cccggttgcc
    73501 acgccacttt ccgtaaacgc ctgctgaaac atggcttcaa catcactcgg taacatcgat
    73561 agcggaatcg atcgtggcgg cggttttaaa ccacgtcgct gattatctga cgcgtaggca
    73621 taacggaaat gggtaatatc cgtctccagc gcattacccg catcagagat aagcggcaca
    73681 ccagaataag gatgccggcc accaaacaag acgtgaaaaa tcagcaacgc aaggccaaaa
    73741 ttatcgtgat tttctgtgcg ttcaaagccg acaaatgacg acaaggtttg tagctctggc
    73801 ggcgtaaaat gcgacacgcc gacttcgcat aaatgcagtg tgccattggc gttaatctga
    73861 aaggagtcgc tatcaatcaa caccactttg ctgtcgcgac ctaccataaa actgttctgg
    73921 tttacgtcac ccacgacgtg cccgtgctcg tgaaccgtag caaaagatga agcaatattg
    73981 cgcgcaacat agagtagaaa atcccacgca caatgagggt aacgttgacg acgatgtgcc
    74041 gggctataga tcatatgaat cggttcttta ccagaaactt ttggcatcat aaaaccgata
    74101 acttttccgc ctcgtccacc gtgaagcgtt gcctgcggcc aggcgacata attcaacaat
    74161 tgcgcgtcag ctgtcgcagc cataaaggca agtttgtcct gttttaaggc gggtggcggc
    74221 gtgtgataaa tcttggcgac gctatcgaca aactcattga tatcataaac cgcgccttca
    74281 ccgcctttgc ccagttcacg gcctggcgta acgcactcac cagtagcagt atataaagtg
    74341 ggtttcatgg taaccacagc gccagtgcca acgttttatc atcatccgta cgctcgttta
    74401 ccgccgggct ggacaaaaac tgctttaaca attcaggtaa taaatcgagt tgttcctgcg
    74461 ttgctgacgc cagtccattg aaaaacgggg taaagaaagg caca
//
LOCUS       DAZPLI010000003        67173 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZPLI010000003 DAZPLI010000000
VERSION     DAZPLI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67173
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.003"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            30..662
                     /locus_tag="ACHUU3_000155"
                     /pseudo
     CDS             30..662
                     /locus_tag="ACHUU3_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            765..1267
                     /locus_tag="ACHUU3_000156"
                     /pseudo
     CDS             765..1267
                     /locus_tag="ACHUU3_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(1334..2791)
                     /gene="nuoN"
                     /locus_tag="ACHUU3_000157"
     CDS             complement(1334..2791)
                     /gene="nuoN"
                     /locus_tag="ACHUU3_000157"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR2080342.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2798..4327)
                     /gene="nuoM"
                     /locus_tag="ACHUU3_000158"
     CDS             complement(2798..4327)
                     /gene="nuoM"
                     /locus_tag="ACHUU3_000158"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR2080343.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4491..6332)
                     /gene="nuoL"
                     /locus_tag="ACHUU3_000159"
     CDS             complement(4491..6332)
                     /gene="nuoL"
                     /locus_tag="ACHUU3_000159"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR2080344.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6329..6631)
                     /gene="nuoK"
                     /locus_tag="ACHUU3_000160"
     CDS             complement(6329..6631)
                     /gene="nuoK"
                     /locus_tag="ACHUU3_000160"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR2080345.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6628..7182)
                     /gene="nuoJ"
                     /locus_tag="ACHUU3_000161"
     CDS             complement(6628..7182)
                     /gene="nuoJ"
                     /locus_tag="ACHUU3_000161"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR2080346.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7194..7736)
                     /gene="nuoI"
                     /locus_tag="ACHUU3_000162"
     CDS             complement(7194..7736)
                     /gene="nuoI"
                     /locus_tag="ACHUU3_000162"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR2080347.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7751..8728)
                     /gene="nuoH"
                     /locus_tag="ACHUU3_000163"
     CDS             complement(7751..8728)
                     /gene="nuoH"
                     /locus_tag="ACHUU3_000163"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR2080348.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8725..11451)
                     /gene="nuoG"
                     /locus_tag="ACHUU3_000164"
     CDS             complement(8725..11451)
                     /gene="nuoG"
                     /locus_tag="ACHUU3_000164"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR2080349.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11504..12841)
                     /gene="nuoF"
                     /locus_tag="ACHUU3_000165"
     CDS             complement(11504..12841)
                     /gene="nuoF"
                     /locus_tag="ACHUU3_000165"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR2080350.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12838..13338)
                     /gene="nuoE"
                     /locus_tag="ACHUU3_000166"
     CDS             complement(12838..13338)
                     /gene="nuoE"
                     /locus_tag="ACHUU3_000166"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR2080351.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13341..15143)
                     /gene="nuoC"
                     /locus_tag="ACHUU3_000167"
     CDS             complement(13341..15143)
                     /gene="nuoC"
                     /locus_tag="ACHUU3_000167"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR2080352.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15237..15899)
                     /gene="nuoB"
                     /locus_tag="ACHUU3_000168"
     CDS             complement(15237..15899)
                     /gene="nuoB"
                     /locus_tag="ACHUU3_000168"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR2080353.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15915..16358)
                     /gene="nuoA"
                     /locus_tag="ACHUU3_000169"
     CDS             complement(15915..16358)
                     /gene="nuoA"
                     /locus_tag="ACHUU3_000169"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR2080354.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16986..17924)
                     /gene="lrhA"
                     /locus_tag="ACHUU3_000170"
     CDS             complement(16986..17924)
                     /gene="lrhA"
                     /locus_tag="ACHUU3_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR2080355.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18849..20066
                     /gene="alaA"
                     /locus_tag="ACHUU3_000171"
     CDS             18849..20066
                     /gene="alaA"
                     /locus_tag="ACHUU3_000171"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR2080356.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20150..20749
                     /gene="yfbR"
                     /locus_tag="ACHUU3_000172"
     CDS             20150..20749
                     /gene="yfbR"
                     /locus_tag="ACHUU3_000172"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR2080357.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20808..22640)
                     /locus_tag="ACHUU3_000173"
     CDS             complement(20808..22640)
                     /locus_tag="ACHUU3_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR2080358.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22727..23375)
                     /gene="hxpA"
                     /locus_tag="ACHUU3_000174"
                     /pseudo
     CDS             complement(22727..23375)
                     /gene="hxpA"
                     /locus_tag="ACHUU3_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(23386..23880)
                     /locus_tag="ACHUU3_000175"
     CDS             complement(23386..23880)
                     /locus_tag="ACHUU3_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR2080359.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23963..24418)
                     /gene="yfbV"
                     /locus_tag="ACHUU3_000176"
     CDS             complement(23963..24418)
                     /gene="yfbV"
                     /locus_tag="ACHUU3_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR2080360.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24756..25958
                     /gene="ackA"
                     /locus_tag="ACHUU3_000177"
     CDS             24756..25958
                     /gene="ackA"
                     /locus_tag="ACHUU3_000177"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR2080361.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26033..28177
                     /gene="pta"
                     /locus_tag="ACHUU3_000178"
     CDS             26033..28177
                     /gene="pta"
                     /locus_tag="ACHUU3_000178"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR2080362.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28367..29887
                     /gene="yfcC"
                     /locus_tag="ACHUU3_000179"
     CDS             28367..29887
                     /gene="yfcC"
                     /locus_tag="ACHUU3_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR2080363.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29920..30462)
                     /gene="yfcD"
                     /locus_tag="ACHUU3_000180"
     CDS             complement(29920..30462)
                     /gene="yfcD"
                     /locus_tag="ACHUU3_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR2080364.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30520..31071)
                     /gene="yfcE"
                     /locus_tag="ACHUU3_000181"
     CDS             complement(30520..31071)
                     /gene="yfcE"
                     /locus_tag="ACHUU3_000181"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR2080365.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31127..31771)
                     /gene="yfcF"
                     /locus_tag="ACHUU3_000182"
     CDS             complement(31127..31771)
                     /gene="yfcF"
                     /locus_tag="ACHUU3_000182"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR2080366.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31907..32554
                     /gene="yfcG"
                     /locus_tag="ACHUU3_000183"
     CDS             31907..32554
                     /gene="yfcG"
                     /locus_tag="ACHUU3_000183"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR2080367.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32611..32973
                     /gene="folX"
                     /locus_tag="ACHUU3_000184"
     CDS             32611..32973
                     /gene="folX"
                     /locus_tag="ACHUU3_000184"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR2080368.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32994..33887
                     /locus_tag="ACHUU3_000185"
     CDS             32994..33887
                     /locus_tag="ACHUU3_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR2080369.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33935..34825)
                     /gene="rpnB"
                     /locus_tag="ACHUU3_000186"
     CDS             complement(33935..34825)
                     /gene="rpnB"
                     /locus_tag="ACHUU3_000186"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR2080370.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLESNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35022..35795)
                     /gene="hisP"
                     /locus_tag="ACHUU3_000187"
     CDS             complement(35022..35795)
                     /gene="hisP"
                     /locus_tag="ACHUU3_000187"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR2080371.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35803..36519)
                     /locus_tag="ACHUU3_000188"
     CDS             complement(35803..36519)
                     /locus_tag="ACHUU3_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR2080372.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36516..37202)
                     /gene="hisQ"
                     /locus_tag="ACHUU3_000189"
     CDS             complement(36516..37202)
                     /gene="hisQ"
                     /locus_tag="ACHUU3_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR2080373.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37292..38074)
                     /gene="hisJ"
                     /locus_tag="ACHUU3_000190"
     CDS             complement(37292..38074)
                     /gene="hisJ"
                     /locus_tag="ACHUU3_000190"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR2080374.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38295..39077)
                     /gene="argT"
                     /locus_tag="ACHUU3_000191"
     CDS             complement(38295..39077)
                     /gene="argT"
                     /locus_tag="ACHUU3_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR2080375.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39343..39912)
                     /gene="ubiX"
                     /locus_tag="ACHUU3_000192"
     CDS             complement(39343..39912)
                     /gene="ubiX"
                     /locus_tag="ACHUU3_000192"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR2080376.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40007..41524)
                     /gene="purF"
                     /locus_tag="ACHUU3_000193"
     CDS             complement(40007..41524)
                     /gene="purF"
                     /locus_tag="ACHUU3_000193"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR2080377.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41561..42049)
                     /gene="cvpA"
                     /locus_tag="ACHUU3_000194"
     CDS             complement(41561..42049)
                     /gene="cvpA"
                     /locus_tag="ACHUU3_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR2080378.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42308..42970)
                     /gene="dedD"
                     /locus_tag="ACHUU3_000195"
     CDS             complement(42308..42970)
                     /gene="dedD"
                     /locus_tag="ACHUU3_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR2080379.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42960..44228)
                     /gene="folC"
                     /locus_tag="ACHUU3_000196"
     CDS             complement(42960..44228)
                     /gene="folC"
                     /locus_tag="ACHUU3_000196"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR2080380.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44298..45212)
                     /gene="accD"
                     /locus_tag="ACHUU3_000197"
     CDS             complement(44298..45212)
                     /gene="accD"
                     /locus_tag="ACHUU3_000197"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR2080381.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45368..46027)
                     /locus_tag="ACHUU3_000198"
     CDS             complement(45368..46027)
                     /locus_tag="ACHUU3_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR2080382.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(46110..46922)
                     /gene="truA"
                     /locus_tag="ACHUU3_000199"
     CDS             complement(46110..46922)
                     /gene="truA"
                     /locus_tag="ACHUU3_000199"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR2080383.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46922..47935)
                     /locus_tag="ACHUU3_000200"
     CDS             complement(46922..47935)
                     /locus_tag="ACHUU3_000200"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR2080384.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48001..49137)
                     /gene="pdxB"
                     /locus_tag="ACHUU3_000201"
     CDS             complement(48001..49137)
                     /gene="pdxB"
                     /locus_tag="ACHUU3_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR2080385.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49236..50231
                     /gene="flk"
                     /locus_tag="ACHUU3_000202"
     CDS             49236..50231
                     /gene="flk"
                     /locus_tag="ACHUU3_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR2080386.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50228..51406)
                     /locus_tag="ACHUU3_000203"
     CDS             complement(50228..51406)
                     /locus_tag="ACHUU3_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2080387.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51681..52901)
                     /gene="fabB"
                     /locus_tag="ACHUU3_000204"
     CDS             complement(51681..52901)
                     /gene="fabB"
                     /locus_tag="ACHUU3_000204"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR2080388.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53060..55066
                     /gene="mnmC"
                     /locus_tag="ACHUU3_000205"
     CDS             53060..55066
                     /gene="mnmC"
                     /locus_tag="ACHUU3_000205"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR2080389.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55187..55465)
                     /locus_tag="ACHUU3_000206"
     CDS             complement(55187..55465)
                     /locus_tag="ACHUU3_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR2080390.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(55499..56047)
                     /gene="epmC"
                     /locus_tag="ACHUU3_000207"
     CDS             complement(55499..56047)
                     /gene="epmC"
                     /locus_tag="ACHUU3_000207"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR2080391.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56047..56856)
                     /locus_tag="ACHUU3_000208"
     CDS             complement(56047..56856)
                     /locus_tag="ACHUU3_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFR2080392.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56856..57680)
                     /gene="mepA"
                     /locus_tag="ACHUU3_000209"
     CDS             complement(56856..57680)
                     /gene="mepA"
                     /locus_tag="ACHUU3_000209"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR2080393.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57684..58769)
                     /gene="aroC"
                     /locus_tag="ACHUU3_000210"
     CDS             complement(57684..58769)
                     /gene="aroC"
                     /locus_tag="ACHUU3_000210"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR2080394.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58804..59736)
                     /gene="prmB"
                     /locus_tag="ACHUU3_000211"
     CDS             complement(58804..59736)
                     /gene="prmB"
                     /locus_tag="ACHUU3_000211"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR2080395.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            59902..60453
                     /gene="smrB"
                     /locus_tag="ACHUU3_000212"
     CDS             59902..60453
                     /gene="smrB"
                     /locus_tag="ACHUU3_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR2080396.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60575..61447)
                     /locus_tag="ACHUU3_000213"
     CDS             complement(60575..61447)
                     /locus_tag="ACHUU3_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR2080397.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(61434..61958)
                     /locus_tag="ACHUU3_000214"
     CDS             complement(61434..61958)
                     /locus_tag="ACHUU3_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2080398.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(61955..62425)
                     /locus_tag="ACHUU3_000215"
     CDS             complement(61955..62425)
                     /locus_tag="ACHUU3_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2080399.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(62422..62970)
                     /locus_tag="ACHUU3_000216"
     CDS             complement(62422..62970)
                     /locus_tag="ACHUU3_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2080400.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(62945..63696)
                     /locus_tag="ACHUU3_000217"
                     /pseudo
     CDS             complement(62945..63696)
                     /locus_tag="ACHUU3_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(63716..66358)
                     /gene="yfcU"
                     /locus_tag="ACHUU3_000218"
                     /pseudo
     CDS             complement(63716..66358)
                     /gene="yfcU"
                     /locus_tag="ACHUU3_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66440..67003)
                     /locus_tag="ACHUU3_000219"
     CDS             complement(66440..67003)
                     /locus_tag="ACHUU3_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2080401.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16727 a        18203 c        16731 g        15512 t
ORIGIN      
        1 taaaataacg tcttttattg ataatttata tatggaaaaa aattcctttt gttatattgt
       61 ttctgatttc actccctctg attattttta ccaaccctgg gtcactgcat tatcgccaac
      121 gccccgacac gtcagtcctg agcaattaga aaaaacagtt cgttatctta cacaaactgt
      181 gcatccacgt aatgccgaca atattgataa tctgaatagg tccgtagagt atataaaaga
      241 gttctttgtc agtagcggtg ccagagttac cgcgcagagc gtccccgatt ggctattacg
      301 actctgcgcc tggtagccag gattaccctt acccgcaatg tcctggcttt atcccgatcg
      361 gggggacttt attgcctggt cggcagaata caggatatca acgccgttcg tcaggtaaaa
      421 acggcattgt tatcatcccg ggatttatct gtttattcta tgaatgcccc atggtttatt
      481 cccggtattg atttctctga ccacctgaat tattggcaac acgatattcc cgccgtaatg
      541 attactgaca ccgcttttta tcgtaataaa caataccact tgcccggtga tacagcagac
      601 agattgaatt atcagaaaat ggctcagatg gtgttataac cttgttacac aacagtaaat
      661 aataaaaatt tctcgaggcg ggtccagaaa cagcgacatg tacattttat tttccctcgt
      721 ctgttttaag atgcgaaaca taaaattcaa tggaggaaaa ataaatggga atgattggtt
      781 attttgcaga aattgactca gaaaagatta accaactgct tgagtctact gaaaaacctt
      841 tgatggataa tatacatgac acgctttcag gattacgccg actggatatt gataaacgct
      901 gggatttttt acactttggg ctgacaggca cccctgcttt tgatcccgcg aagaatgatc
      961 cgctgagtcg tgccgtgctt ggtgaacaca gcctcgaaga tggcatagat ggcttcttgg
     1021 gattgacctg gaatcaggag ttagccgcga caatcgaccg actggaatca ctggatcgaa
     1081 gtgagctgcg taaacagttt tccattaagc gactcaatga aatggagatt tatccgggag
     1141 tgaccttttc agaagagtta gaggggcaac tctttgcttc gatcatgctg atatggaaaa
     1201 gctgattagc gcgtatcgac gtatgctgcg acaagggaat catgcgctga cggttattgt
     1261 tgggtgatgg tgtcagggca ccaaaaaaaa ccgccgaaca tgtcggcggc tttctgactt
     1321 acaaactgat agcttacatc agcggcattg ctaaccgcac aatgctaatc agcggttgtg
     1381 gccatacacc cagcaccagt accaacagtg cagagatcag caccacgata ccgcctgcgc
     1441 tgtactgcca gtttgacggt gcatcgcgac ccggttgttc cggggcgtgc aggtacaggc
     1501 tcaccgccac gcgcaggtag tagtaaaggc cgcttgccga accgacaacc acggcgccca
     1561 ccagccacca caagtgtgcc tggacaccga ccgccagcac gtagaactta ccaataaagc
     1621 ccagcgtcat cggaataccg gccagagaca gcatcatcac cgtcatcacc gccgcgagga
     1681 tcgggcgatg ccagaacagt ccgcggtaag agtacaggga atcagcatcc gggccacgat
     1741 acgggctgga catcaggctg accacgccga acgcgccgag gctgctaaac agataaccgg
     1801 ccaggtaaac ccctaccgct tccatcgaca tctcgccagt ttgcagcgca atcagcgcca
     1861 ccagcagata gccgaggtga gagatagatg agtaaccgag cagacgtttg atattggtct
     1921 ggctcagcgc catcaggtta ccgaagatga tggaggcaaa ggcgataatc gccagcacca
     1981 cgcgaatcgc ttcgctgtca cccatcggtg cgtagaggaa cagacgcatc accacaccga
     2041 agatagcgat tttgctcgcc gtcgccagga aagtggaaac cggcgcaggc gcgccctggt
     2101 atacgtctgg cgtccacagg tggaacggca ccagagagag tttgaagccg aggccaacaa
     2161 tcatcaggcc gaaacctgcc agcaacagcg gctcgttgag cataccgtcg ccaaggtttt
     2221 tacccaacgc gacaaacgac aggtcgccag actgcgcata caccagcgcc ataccaaaca
     2281 gcaggaaaga agacgctgcg gcagaaagga tggtgtattt gatactggct tccagtgaac
     2341 gtttctggcg gaaagcgtaa ccgaccaggc caaacagcgg caaagagatc agttcgatac
     2401 cgaggaacag agacgccaga tggttggcat tcgccagcag gatcccgccc agcgcggcaa
     2461 ttaacaccaa caggtagaac tcgtccttgt tgtcgttata gccttcaagc cacgggtagg
     2521 cgaaagtaca ggtggcgagg ctcgccaaca ataccagccc ggtgtaaagc atggcgaaac
     2581 catcaacgcg catcagcggc gtaacgtcca tagcgcctgc ctggccgaca aaccagagcg
     2641 aaaccagcgc cgcgttaagc ccaataaccg agagcgtagc gttgaggaaa tgattgcgtc
     2701 gccacgcaat ggagagcatc acaaccacca ccgtcaagcc gacgatcagc aacggtagca
     2761 gtgcgatcag gttttgtgga gttattgtca tggcgattta cggccttgta gtagtaacgg
     2821 aattaacaaa ccactgctgg atattgccaa tcgcggagtg cgaggtatcc agaatcggct
     2881 gcggatagaa gccctgcagt accagcagca ccaccagcaa caggatcata aacagctcac
     2941 gcagcgacat ccctggcagt tcctggctgg caatctcgct tttcgcttta ccgaagtaag
     3001 cgcgatgcaa catcgccagc gaataaacag atgcaaagac cagcccaaag gtagagataa
     3061 cggtaatcac cgggacaacc tggaagctgc cgaacagaat cataaattcg ccgacgaagt
     3121 taccggtgcc aggcatccca agcgttgcca ccgcaaagaa cagcgacagt gctggcagcc
     3181 atttcatctt gctccacaga ccgcccatca tgcgcatgtc gcgggtatgg atacgttcat
     3241 aaagctgacc acaaagaata aacagacccg ccgccgacaa gccgtgcgca atcatctgga
     3301 ttaccgcgcc ctggtaggcc aactggctgc cggtgtagat agcaatcagc acgaagccca
     3361 tgtgggaaac cgaggtgtag gcgatcagac gtttgatatc ggtctgggcg aaggccatcc
     3421 acgcaccgta gaagatgccg ataacaccca gccacatagc aattggcgcg aactctgccg
     3481 acgcgttcgg gaacagcggc agggagaaac gcagcaaacc gtaagcggca gttttcagca
     3541 agatccccgc gaggtcaacg gaaccggcgg tcggagcctg ggagtgcgca tccggcagcc
     3601 agccatgcag cggaaccacc ggcattttga ctgcgaaggc gatgaagaag cccagcatta
     3661 acaggtattc cgcaccactg gacattggcg tattcagcag ctcttcatag ttgaaggtcc
     3721 agacgccggt cgcattgtag tgaacaaaaa ccagcgccag gatggcaatc aacatcacca
     3781 gaccactcgc ctgggtgtaa atgaagaact tggttgccgc cgtgatacgc gttttaccgt
     3841 cagaggcttt atgcccccac agtgcgatca ggaagtacat cggcaccagc atcatttccc
     3901 agaagaagaa gaacaggaac atgtcgatgg caaggaacac gccgataacg ccgcccagga
     3961 tccacatcag gttgaggtgg aagaagccct gatatttttc gatctctttc cacgaacata
     4021 gtaccgccag cacaccgagc agaccggtca gcacgaccat cagcagcgac agcccgtcaa
     4081 tggcgagatg aatagagata cccaaacgcg ggatccacgg catgtcgaat tcagactgcc
     4141 actgcggaat tccggcggat tgcgtcagtg aataaccgcc ctgcaaccac agttgcagcg
     4201 acagcgccag cgtcaatccc atggtgatca gcgcgatcca gcgtggcacc ttgacgccaa
     4261 agcgttcggt ctgccagcac aggaagccgc caataaaggg aattaatatt agccagggta
     4321 gtaacatggc gatctttatt ccttgtaaaa gtcccgtcag gaccggattt tcaacgaatt
     4381 ctcacgacag aaaattctct tcggactggg ggtagtgtcg gatgcaccgc gaaggcggct
     4441 tatccgacct acgttcttat atgcccaggg gcaatcccac aatccttaac tcaacgcagt
     4501 accatcaaca gtgccagcac cacgaccgca ccgatgctca tggatgccac ataccagcgc
     4561 agatagccgt tctcacttaa cagcagacct ttacctgcaa agcgggaaag gacagccgga
     4621 atgttcatca ttgagttcag cggatcgcgt ttcagcaacc aggcaatacc caggaacggc
     4681 ctgacgaaca ctttgtcgta cagccagtca aagccccagg cgttgtacca ccaggtactc
     4741 agcaggcggc ccggcgcact gttggcgatg gaggtcacca gagtacgttt acccagccac
     4801 agccaagctg ccagcagaat gccgacaatc gcgaccacgc cagaggtaat ttccagggtc
     4861 aacatgctgc cgtgcgccag ttccgtcgtt tgcggaagca cgccctgcag cggcggtaca
     4921 atcagtgcgc caacgaaggt ggaaaggatc agcagcacaa tcagcggcag gctgtgagtt
     4981 acccctttca cggcgtgagc gtgaatttgt tcttttccgt ggaagacgat gaaaatcata
     5041 cggaaggtgt agagcgaggt cataaacgca ccgaccagac ctgccaccat cagattgata
     5101 tgaccattcg ccatcgcacc cgcgaggatc tcatccttac tgaagaagcc cgcagtgacc
     5161 agcggcagtg ccgacagtgc tgcgccgccc accaggaagc agagataaac cagcggaata
     5221 gatttacgca gaccgcccat cttgaagatg ttctgttcgt gatggcaggc cagaatgacg
     5281 gaaccggatg ccaggaacag cagcgcttta aagaacgcgt gagtcatcaa gtggaaaatc
     5341 gccgcatccc atgcctgcac gccaagcgcg aggaacatgt agccaatctg gctcatggta
     5401 gagtaagcga gaacacgttt gatgtcggtc tgtaccagcg cggcaaaacc ggccagcagc
     5461 agcgtaaccg ccccgacaat gcccaccaga tgcagaattt ccggcgtcat caggaacagg
     5521 ccgtgggtac gggcgatcag gtagacaccc gcggttacca tggttgcggc gtggatcagc
     5581 gcggagacag gcgtcgggcc cgccatcgcg tcggcaagcc atgtctgcaa cggcaactgc
     5641 gcagatttac cgaccgcacc gcccagcagc atcagcgtcg cccacatcag catgttattg
     5701 ccgtcagcaa agtgcgctgg tgccagttcc accatttcgc ggaagttcag ggtgcccagt
     5761 tcgttgtaaa gaatgaacag tgcgaaagcg aggaaaacgt cacccacacg ggtcacgacg
     5821 aacgctttca ttgccgctgc gccattcttc ggatcggtgt aatagaaacc gatcagcaga
     5881 taggagcaca ggcccacgcc ttcccagccg aggtacatca gcagcaggtt gtcggcaagc
     5941 accagaacta ccatgctggc gatgaacagg ttggtgtagg cgaagaagcg agagtagccc
     6001 tcttcaccgc gcatgtacca ggaggcgtac atgtgaataa ggaaacccac accagtgacc
     6061 accgagagca tggtcagcga caggccgtcc agcaccaggt taaaaccgat gttaaagtcg
     6121 cctaccgaca tccacgtcca cagcggctgg ctgtatgcct gctcgccgtt agcgaagaaa
     6181 tcaacgccga taaaggcggt taccagcgcc gccaggccca cagagccaac gccgacgatc
     6241 gccgaaacgt tttcagacca gcgcccacgg gagaacgcca gcaggacgaa gccaatcaat
     6301 ggcaaaataa tggttaaggc aagcatgttc atccgcgcat ctcacttact gaatcgatgt
     6361 tcaggttctg gcgacgacgg tgaagttgca gcagcagcgc aaggccgata ctcgcttctg
     6421 ccgccgcgag gctgatggcg agaatgtaca tcacctgacc gtcggtctga ccccagtagc
     6481 ttccggcgac aacaaaggcc agcgcggagg cgttaatcat gatttccaga ccaatcagca
     6541 taaacagcag attgcgacgg ataaccagac cggttaagcc aagaatgaat aagattgccg
     6601 cgaggatcag tccatgttgt aaggggatca tgcgtgctcc tccgtttttc ttttcgcgct
     6661 gtcgtcttta cgattgctca gcacttcacc cgcacgctct tcacgaccga cgtggaaggc
     6721 cacaaccaga cctgcgagca gcagcataga agccagttcc accgccagta cgtaaggccc
     6781 gaacagcgta atacccactg ctttagcact gattggcgta ccgtcgatac cctgatcgtt
     6841 aacaccgagg atggcgtaaa caatcaccac cagcatgatg gccgacaaaa ttgccggacc
     6901 aatccacacc tgcggtttca gccactggcg ttcctgttcg atttctgaac cgcccaggtt
     6961 gagcatcatc accacgaaca cgaacagcac cataatggca cccgcgtaga cgataatttc
     7021 cagcgcaccg gcgaagtaag cgcccagtga gaagaacacc ccggaaatcg ccagcagcga
     7081 aataatcagg tacagcagtg cgtgtaccgg attggtatgg gtgatcactc gcaaggtcgc
     7141 aagtatggct atcaggccac agatataaaa agcgaactcc attgcccctc tccttacggt
     7201 aacaggctct tgacgtcgat aggcttggct tcgttctctg cttcgccctt atctttgccg
     7261 tcgattgcca tacctgccat ccggtagaag ttatattccg ggtatttgcc cggaccggag
     7321 atcagcagat cctctttttc gtaaaccaga tcctggcgct tgtattcccc catttcgaaa
     7381 tccggggtta actgaatcgc cgtggtcgga caggcttctt cgcacagacc acagaaaatg
     7441 cagcgtgaga agttgatgcg gaaaaattcc ggataccagc gaccgtcttt ggtttctgct
     7501 ttttgcagcg agatacagcc gaccgggcag gctaccgcgc agaggttaca ggctacgcaa
     7561 cgctcttcgc cgtccgggtc gcgggtcaga acgatacgac cacgataacg gggcggcaga
     7621 tagatcggct cttccgggta cattcgcgtt tcgcgtttgg cgaacgcgtg caggccgatc
     7681 atccagatac tacgaacctg ggtgccgaaa cctactaaca attcttttaa ggtcatggtc
     7741 ttattgcccc ttattgcgcc tgccagagaa tgacagccgc cgttaccagc aagttgatca
     7801 gcgtcagcgg caggcagatt ttccagccga aggacattac ctggtcataa cgcggacgcg
     7861 gtaacgacgc acgaatcaaa atgaacatca tcataaagaa cgcggttttc agcgcgaacc
     7921 agatgaatgg cggtaacaac gggccttgcc agccaccgaa gaacagcgtc accatcaatg
     7981 cagagatggt cacaatcccg atgtactcac ccacgaagaa cagaccgaac ttcataccgg
     8041 aatattcaat gtggtaacca tccgccagtt cctgctcggc ttccggctgg tcaaacgggt
     8101 gacggtgaca taccgccacg cccgcgatgg caaaggtaat aaaaccaaag aattgcggga
     8161 taacgttcca cacatgcgcc tggctgttga cgatgtcggt catgttgaat gaaccggcct
     8221 gcgccaccac gcccatcagg gaaagcccga ggaacacttc gtagctcagg gtctgcgcag
     8281 aagcacgcat cgcacccagc aacgagtatt tgttgttact tgaccagccc gcaaacagca
     8341 ccgcgtaaac cgccagacct gccatcatca ggaagaacaa aatcccgatg ttcaggtcgg
     8401 caaccaccca acccggactg actggcacaa tcgcaaaggc cagcagcagc gaggtaaagg
     8461 caatcatcgg tgccagggta aagatgacgc gatccgagaa tttcgggatc cagtcttctt
     8521 taaagaacat tttgatcatg tccgcaacca gctggagcga accgccccag ccaacacggt
     8581 taggtccgta acggttctgg aacagaccca gcaggcgacg ttcgccaaag ctcatgaatg
     8641 ccccgcaggt gacaaccacc agcaggatca ccaccgcttt gaggatggtc agcaggatct
     8701 caatcagttc cggtgatatc caactcattg ttgtgcctcc ttgagatcct caagatgcgc
     8761 gccagccagc accggagcaa taccggacat acccatcggc aagcccacct gccctgccgt
     8821 cagcccttca gcgatttcaa ccggcagcgt gaccgtattg ccatcgtaac taaaggagac
     8881 gtgtgtacct gcgttcacac ctaacttcgc ggcatccgct gggttgagtt tgatgtacgg
     8941 ctgcggcata cggctctgga agaccggagc acgctgtgac aattcatcgc tgccaaacag
     9001 gtggtaatac ggcgcgatac gccatttccc gtcctgcggc tggaagcgtg ccggtacgct
     9061 ggtgaagtaa tccagaccat tttcgctggt ttcaaacaga cgcacgcccg gatcgccaaa
     9121 gcgcagtttg ccgcccactt cgtcctggaa tttgttccac gcctgcgggg agttccagcc
     9181 cggcgcccag gcaaacggca cttgcgaacg gtgcgcagtc ggctggttgt taccttccat
     9241 cgagaaggtg aacatggtgt caatatcctg cggctgacgc ggctcatgaa cgctgatatt
     9301 ggcgcgcatg gcggtacgac cgctgtaacg gtgcggttca cgggccagtt tctgcccacg
     9361 aatacggaat gtcgcatccg gcgcagcatc tttgatacct gccagttccg ggattttcgc
     9421 cacaacagcg tcaatcacat ggtcgagctg cgtccagtcc acttcacggc tcagcagggt
     9481 gctgtgcagc gagtgtaacc agcgccagct ttccagcatg acagttttgc tgtcgtaata
     9541 agcaggatcg taaacctgga agaaacgttg ggcgcggcct tcgttgttga tcaccgtacc
     9601 gtcgctttca gcaaaactgg cagcagaaag taccagatgg gcgttttcca taatcgctgt
     9661 gcgttgatga tcaaccacca tcaccagcgg cgctttagcc agcgcagcat tcacgcgggt
     9721 agcggaagcg tgacgatgca gatcgttttc cagcaccacc accgcgtcgg cgcgtccggt
     9781 ttccagttcg gtcaacgctt cttcaagcga accgccaccc ataatgccca gccccatgct
     9841 gttgacggaa cgggcaatca tggtgatacc aacgtcagcg ccgcgacctt tcagggcttt
     9901 cgcgacgtta gccgcagcct gaatcacctc tatgctaccg gcgttcgtcc cggagataat
     9961 caacggtttc ttcgcacctg ccagtgcctg cacgatgacg tcgattttgc tttgcagctc
    10021 aggttcgata ccgtcaaccg ctggcgcaga gttatccagc gcatgggcga tggcaaaacc
    10081 taaacgcgcc tgatcttcaa ccggtgcgcg gtaagtccac gccgcgatat catccagacg
    10141 ggtgtcatca acgttggtaa caaacagcgg atgcttcgca cgttgaccga tgttgaggat
    10201 tgccgcaatc tgccagtcag ccactttctg tgctgccgcc atttcgcgcg ctttaccttt
    10261 cacagcctga cgcactgcca gcgcgacgcg cgcgccggtc tgggtaacgt cttcgcccag
    10321 caccagtacc gcatcgtaag attcgatttc gcgcagagcc ggagtataaa tgccgccttc
    10381 acgcagcact ttcagcgcca gttgcagacg ttcctgctca ccgtgagcga taccggtgta
    10441 gaagttttct tcgcccacca gttcacgcag cgcaaagttg ctttccacgc tggcacgcgg
    10501 agaaccaata ccgatcactt tcttcgactg acgcagaata tctgccgcgc cctgcattgc
    10561 ctgttcggcg ttgagggtaa tgaaatcatc gccacgacgc tgtactggct gacgcggacg
    10621 atccttcagg ttgacgtaac cgtaaccgaa acgaccacgg tcgcagagga agtagtggtt
    10681 taccgtaccg ttgtaacggt tttcgatacg acgcagttcg ccgtaacgtt caccggggct
    10741 gatgttacag ccgatggaac attgctggca gatgctcggc gcaaactgca tatcccattt
    10801 acggttgtaa cgctcggagt gcgttttgtc ggtaaatacg ccggtcgggc aaatttcgac
    10861 caggttaccg gaaaattcgc tttccagcgt gccgtcttcc gggcgaccga agtagacgtt
    10921 gtcgtgcgca ccgtaaacgc ccagatctgt accgtcagcg taatctttgt agtaacgcac
    10981 acagcggtag caggcgatgc agcggttcat ttcgtgagag atgaatggcc ccaaatcctg
    11041 attacggtgg gtacgtttgg tgaaacggta gcgacggaag ctgtgtccgg tcatcacagt
    11101 catatcctga agatggcagt taccgccttc ttcacatacc ggacagtcgt gcgggtggtt
    11161 ggtcattaac cactcgacca cactttcacg gaactgtttc gcttcttcgt cgtcaatgga
    11221 aataaaggtg ccatcggaag ccggtgtcat acaggacatc accaggcgac cacgcgtgtc
    11281 ttccgcgttt tggtattgct tcaccgcaca ctggcggcaa gcaccgacgc ttcccagcgc
    11341 cggatgccag caaaagtaag gaatatcaag gcccagagac agacaagctt ccagcaggtt
    11401 gtccgctccg ttgacctcgt attctttgcc gtctacatga attgtagcca ttagcatgct
    11461 tccagttttc tcggtcaggg accgagcgtt aatcgaaatt cggttaccag cgctctttca
    11521 gcaggttcgg ctgaatccca ttaatcaaat gggtattgct gaacggctgt ttgattcccg
    11581 cctcaaattc ttcgcggaaa tatttgatgg cgctctgtaa cggctccact gcaccaggtg
    11641 cgtgggcaca gaaagtttta cccgggccta agaatcgaca cagttgctca agtgtttcga
    11701 tatcgcccgg ctggccttcg ccacgctcca gcgcacgcag aattttcacg ctccacggca
    11761 gaccgtcgcg gcacggcgta caccagccgc aggactcacg ggcgaaaaac tcttccaggt
    11821 tacgcaccag cgacaccatg ttgatctcat ggtcaaccgc catcgccagc gccgtaccca
    11881 gacggctgcc cgctttaccg atactttcga attccatcgg cagatcaagg tgcgcttcgg
    11941 tcaggaagtc agtccccgcg ccgcctggct gccaggcttt aaatttcaga ccatcacgca
    12001 taccaccggc gtaatcttcg aggatctcgc gtgcggtggt gccgaacggc agttcccaca
    12061 gtcccggatt tttcacccga ccggagaagc ccatcagctt ggtgccagca tctttacttt
    12121 tcgagatgtt ctgataccac tccacgccgt tagcgaggat cgccggaacg ttacacaggg
    12181 tttcgacgtt gttgacacag gtcggtttgc cccatacgcc ggaggttgcc gggaaggggg
    12241 gtttcgagcg tgggttagca cgacgccctt ccagggagtt gattaacgct gtttcttccc
    12301 cgcagatgta gcgccctgcc ccggtatgga cgaacagttc gaagtcgaaa cctgttccca
    12361 taatgttttt gccaagcaag cccgcttcag tggcttcggc aatggcgcgg cgcagattta
    12421 ctgccgcttc gatatattcg ccacgcagga agatgtagcc acggtaagct ttcagcgcaa
    12481 acgcggagat gagcatacct tccaccagca ggtgcggcag ttgctccatc aacaggcggt
    12541 ctttataggt gcccggctcc atttcatcgg cgttacacag caggtaacgg atgttcatgg
    12601 attcgtcttt cggcatcagg ctccacttca ggccagtcga aaagcccgct ccgccgcgcc
    12661 ctttcagacc agcgtctttt acctgattaa cgatttcgtc cggagacagc ccggtcagcg
    12721 ccttacgcgc cccttcgtaa ccgtttttgc tgcggtattc gtccagccac actggctgtt
    12781 tgtcatcgcg cagacgccag gtcagcggat gcgtttcggg agtacggata atgtttttca
    12841 tttataccgc tccagcagtt cagggatcgc ttccggggtc agatgcgcgt gagtgtcctc
    12901 atcgatcatc atgtttggcc ctttatcaca gttccccagg cagcaagttg gcagcagcgt
    12961 aaagcggcca tcaaatgtcg tttgccctgg tttgatgttc agctttttct cgagcgccgc
    13021 ctgaataccc tgatacccgt tgatatgaca gaccacgctg tcgcaataac ggatcacatg
    13081 gcgaccaacc ggctggcgga agatctgact gtagaacgtt gccacacctt cgacgtcgct
    13141 tgccggaata cccagcacat cggcgatcgc gtggatcgca ccatccggca cccagccacg
    13201 ctgcttctga acgattttca gcgcttcaat ggacgccgca cgcgggtctt cgtagtggtg
    13261 catctcgtgc tcaatcgctt cacgctctgc cgcactcagc tcaaaagcct cggtttgtgg
    13321 ttgttgattc tcgtgcataa ttagcggtcc acatctgaca taacaaaatc gatactgccc
    13381 agataaacaa tcaggtcaga caccaggctg ccgcggatcg ccgccggaat ttgctgcaaa
    13441 tgcgcataac tcggcgtgcg gatacgagtg cgataactca tggtgctacc gtcgctggtc
    13501 aggtagtaac tgttgatccc cttggtcgcc tcaatcatct ggaaagattc attggcaggc
    13561 atcaccggcc cccacgacac ttgcaggaag tgggtgatca gggtttcgat atgttgcagc
    13621 gtgcgctctt tcggcggcgg cgtggtcagc gggtgatccg ctttgaacgg gccttccggc
    13681 atgttgttga ggcactgctc aagaatgcgc agactctggc gcagctcttc cactttcagc
    13741 attacgcggg tgtagcagtc agaaacacca ccaccaaccg ggatttcaaa gtcgaagttt
    13801 tcatagccag aataaggacg cgccttacgc acgtcgaagt cgatcccggt agcacgcagg
    13861 cccgcgccag tggtgcccca ctccagcgcc tctttcgcgc cataggcggc aacgccctgg
    13921 gaacgacctt tcagaatggt attttgcagc gccgctttct cgtaagacgc cagacgtttc
    13981 ggcatccagt cgaggaactc acgcagcagg cgatcccagc cgcgcggcag gtcgtgcgct
    14041 acgccgccaa tacggaacca cgccgggtgc atacggaaac ccgtgatcgc ttccaccaga
    14101 tcgtaaattt tctgacgatc ggtaaaggcg aagaacacgg gcgtcattgc gccgacgtcc
    14161 tgaataaagg tcgagatgta cagcaggtgg ctgttgatac ggaacagttc ggagagcata
    14221 acgcgaatga cgttaacgcg atccggcacg gtgatcccgg ccagtttctc taccgccagc
    14281 acgtaaggca tttcgttaac gcagccgccg aggtattcaa tacggtcggt atacggaatg
    14341 tagctgtgcc aggactggcg ctcgcccatt ttctccgcac cacggtggtg gtagccgatg
    14401 tctggtacgc agtcgacaat ctcttcgccg tcgagttgta aaacgatacg gaaagccccg
    14461 tgcgccgacg ggtggttcgg accgaggttg aggaacatga agtcctcgtt ttcggtgccg
    14521 cgcttcatcc cccactcttc cggtttgaag gtcagggctt ccatctccag atcctgtttg
    14581 gctttggtca gctcaaacgg cgagaattcg gtagcgcgcg ccgggtaatc tttacgcagc
    14641 gggtgacctt tccaggtttg cggcatcatg atgcggcgca ggttcgggtg accgtcgaaa
    14701 gtaatgccaa acagatccca ggtttcacgc tcataccagt tagcgttcgg gaacagtttg
    14761 gtgaaggtcg gtacgtgcag gtcgttttct gccagcgcca ccttcagcat gatgtcgcgg
    14821 ttacgatcga tagaaatcag atggtagaaa acggaaaaat ccgcggcagg taacccttcg
    14881 cggtgtgtgc gcagacgttc gtccatgccg tgtaagtcaa acagcatgac gtaaggtttc
    14941 ggcagtttct ttaagaaatc gccaacttcc agtaattgtt cacgcttgat ccacacaacg
    15001 ggaaccccgg tgcgagtcgc ctgaacagta aaggcatccg gcccaaaacg gttgcgcagt
    15061 tcgccaatca ccggatcatc aagatgatca cgggtctgcc aggcgggttc ttgcgcggtt
    15121 aagtcggtca tattgttcac cattgcaaat ggtccgtggt gactgtcggc gcagtaagct
    15181 tcgcgaaata gatttaggaa taagcgaaga aaaatccctt tgccgacagg cgcaaattaa
    15241 atctcgtcag gtgtacgcag gttggttacg gcaatgcgtt caccgcgctt gcgttcgcgc
    15301 tctgattgca tattggcgcg ataaacgccc tgatcgccaa ccacccagga gagcggacga
    15361 cgttctttgc cgatagattc ctgcaacagc atcagtgcct gcatgtacgc ttcaggacgc
    15421 ggcgggcagc ccgggatata cacatcaacc gggatgaatt tatcgacgcc ctgcacaacg
    15481 gaataaatat cgtacatacc accagagttg gcacaggcac ccattgagat aacccatttt
    15541 ggttccagca tctggtcata cagacgctga ataaccggtg ccattttggt aaagcaggtt
    15601 cctgcaacca ccatcaggtc agcctgacgc ggcgaagcac gcaatacttc tgcgccaaaa
    15661 cgcgccacgt catgcaccgc ggtaaacgaa gtcaccatct caacgtaaca gcaggaaaga
    15721 ccgaagttat acggccaaat tgagttttta cgaccccagt taaccatgtc attgagcttg
    15781 cccataaaca cgtttttgtt aacttcttgc tccagagggt cggttacgat ctcctgcttt
    15841 tgcagggggt aacggtcgtt ctcaccgttg ggatctatgc gggtgagcgt ataatccatc
    15901 ttaatgcctc gcggttagcg ttgacgatta gcgatactgt tcgtttccgg gttcatacgc
    15961 tcgcggcgtg aacgcgcggg cgtccagtcc agcgcgccaa tacgcaccag ataaaccaga
    16021 cctgccagta acacaaaaat aaaaattgca gcttccacaa agcctaccca gccgctttcg
    16081 cggatagagg ttgaccatgc gaacagatac agcgcttcaa cgtcgaagat aacgaagaac
    16141 atggccacca gataaaactt ggcggacagg cgtaagcggg cggagccgac cgagtcgata
    16201 ccggattcaa acggcacgtt tttcgacctc gcgcgtgcgc gaccgcctaa aaaccaaccg
    16261 cctaccagca tcaggcaaca caggccaatg gcaacgataa gaaagatagc gaatgcccag
    16321 tgatgagcga tgacttcagt ggatgttgac atactcattg cttactcatc aaaagtagcg
    16381 ccagattcac tgctcttcac ggcagatgga cgccacatcg attcatgggg aggaataaaa
    16441 aaaaccttac aatcactgta gaaattcttt tatacagcta attgatgtgg tcttttactc
    16501 ctttctataa ccttttgtca actttaacaa aagtttcttc acattagttt acataatatc
    16561 aacaccatta gcatttaatg ccctttcacg ccagatcctt gacgactcca ggataattag
    16621 atgttgttga tcgtgtccgt tgtgaagcaa tggaaaaaat acgggtctat tttgacagga
    16681 atttgtgtcg attcctcccc ccaaaagaga gtattttctt gatctgtgac acgcttttgt
    16741 cattccataa caaaaacgca gcaacaaatt tacgtatttt taacatcatt gtagcaggtg
    16801 atttttttca ggcgattatt tgtgcgttcg ggacgtgaat ctctggtgat tgaaaaatga
    16861 acagttttgt acgttctgca ctatgcgagg aaggattttt actaaaaaaa agccgctggg
    16921 gtttaaaaca cccccagcgg ctcgttttta cactattgtc tcaggaatta tctatcgtcc
    16981 gtcgattact cgatatccct ttcaatcaac aatgaatcat ccccttccgg agcagacatt
    17041 gggctgtatt gccacggatt atggtagctc tccatcgcct gatagatcac ctgggccagc
    17101 tcattattac tggacgggtc atagcacagc aaatattcgg tatcaggtag cggcggtaag
    17161 ccatctactc cactcagcac acgcaggtcc gggctcatca tttcaatcgg ccttgccgtc
    17221 acgccaagac cggctttcac tgccgcacga acggccggaa gcgtcgaggc gacataagcc
    17281 agtcgccatg gaatatctgc tttattaagc gtcgccagca ccatatcgcg aaacggactt
    17341 ggatcatcca gcaatacaag agggatcggc tcgccttttt gcaatacgta ttccgctgcg
    17401 cagtaccagt gtgttggcga ggtacgcagg ttcaatgcct taaacgctga aggacgatgg
    17461 gtggttacca tcaaatccac ttcctgggat tccagcattt ctgccatata ggcattacgt
    17521 ttaacgcgga catccagcgc taatttcgga taaaccgaac tcacgcgatt taacaggaaa
    17581 ggtaagatcg tatcggcaga ttcatctgaa gcaccgatag ttaacacgcc ctgaagatta
    17641 ctgaacatta atgatgagca ggcctcatca ttaaaacgca gtattttcct ggcgtaacca
    17701 agaagttgaa tgccatgttc agttaacagt ttgttgcgac cgtgacgagc gaacagttct
    17761 ttcccaacga gttgttccag acgctgcatt tgctgactta cggcggactg agtacgacac
    17821 acagcggcag ctgcggcagc aaaagtgttc agatcggcaa cagcaacaaa tgttctcagc
    17881 agatcgaggt cgaggttaat tatcggacga tttgcactta tcatatcata tattatcact
    17941 tactggcggc tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt
    18001 gtaatgtgcc tccctggcag tttcatccgg aacccggacg aaagtaaaaa tgcatatgag
    18061 ttgcactaaa aaagcgactc acattgttcc gttataatgc ctgaagtaga tcacagaata
    18121 tatcttcagg gatcgcatat ctattaagtt actcactctt ttctatttat gacatgcgcg
    18181 tgtttgtata aatgtaaatg tgagtccttg ttccactctc gtgcagcatc gctggtcata
    18241 cgcgaacacg tacaaacagc aatggtgagg cggcatcaag agcagggatc cgtcatttat
    18301 gcgagcattt taccccaaaa accttttatt tataagggtc attgcgaatt atctgatgca
    18361 aagttatgtt atgttaggca aagtaatctt ctattattaa taagcacatc aaaacctttt
    18421 tgaatattaa ataataatta attagcatca tcctcattca ttaattccgc ttaacaatag
    18481 tttcacacct tgcccccatt gcgacacctc ggaaatcatc acgctgttaa actctccgta
    18541 atgcggaaat atcatcatcg cgtttcatta gtgaattctt aaatgaggca ttttcacaca
    18601 attatcttac agataaaaaa accagacttg caattaagaa tcagaacaat tcactatata
    18661 acattgcatg caaagcatat acacctcatt attttgtcat tattaagtta ttaacagcac
    18721 aatcgagcct tcccctctgg caaaatctta ttctgcagac cttcaaaaca ccgtcctggg
    18781 ggagtacatt gttctaagct gacttccacg gcagggagtg gcgataacag caaaaaaggt
    18841 caagattcat gtcccccatt gaaaaatcca gcaaattaga gaatgtctgt tatgacatcc
    18901 gtggtccggt gctgaaagaa gcaaaacgcc tggaagaaga aggtaacaag gtactgaaac
    18961 tgaacatcgg caacccagcc ccattcggtt ttgacgcgcc agatgaaatc ctcgttgacg
    19021 tgatacgcaa cctgcctacc gctcaagggt attgcgattc caaagggctt tactccgcgc
    19081 gtaaagccat catgcagcac taccaggctc gtggcatgcg tgatgttacc gtggaagata
    19141 tttacatcgg caatggtgta tcggagctta tcgttcaggc aatgcaggca ttgctgaaca
    19201 gcggggacga aatgttggtt cctgcaccag attacccact ctggaccgcg gcggtttcgc
    19261 tttccagcgg taaagcggtg cattatcttt gcgatgaatc ctctgactgg ttcccggacc
    19321 tcgatgatat tcgcgctaaa attacgcctc gtacgcgtgg gatcgttatt atcaacccaa
    19381 ataacccaac cggcgcggta tattccaaag agcttttaat ggagattgtg gagattgcac
    19441 gtcagcataa tctcattatc ttcgccgatg aaatttatga caaaattctc tacgacgatg
    19501 ctgagcatca ctcaattgcg ccactggcac ctgacctgct gaccattacc tttaacggac
    19561 tgtcgaaaac gtaccgcgtt gcaggcttcc gtcaggggtg gatggtgttg aacgggccga
    19621 aaaaacacgc caaaggctac atcgaaggtc tggaaatgct ggcttcaatg cgcctgtgtg
    19681 ctaacgttcc tgcgcaacac gccattcaga ccgcgctggg tggttatcag agcatcagtg
    19741 aatttattac ccctggcggt cgtctttatg agcagcgtaa ccgcgcgtgg gaactgatca
    19801 acgatattcc gggcgtttcc tgcgtgaaac ctcgtggtgc gctgtatatg ttcccgaaaa
    19861 tcgacgccaa acgctttaac attcacgacg atcagaaaat ggtgctggat ttcctgttgc
    19921 aggaaaaagt tctgttggtg caagggacgg cattcaactg gccgtggccg gatcacttcc
    19981 gcattgtcac gctaccgcgt gtcgatgata tcgagctgtc tttgagcaag ttcgcgcgtt
    20041 tcctttctgg ttatcatcag ctgtaatctt aatttcactg ccggagattg catccggcag
    20101 cgttatcccg ccacaatgac ctgatgatgt catcatacgt aaggtcacta tgaaacagag
    20161 ccattttttt gcccatctct cccgcctgaa actcattaac cgctggccgc taatgcgcaa
    20221 cgtgcggacg gaaaatgtgt ccgaacacag tttgcaggta gcgatggtcg cccatgcgct
    20281 ggcagctatc aaaaatcgaa aatttggcgg taatgtcaac gccgaacgta tcgctttact
    20341 ggcgatgtac cacgatgcct cagaagtgct caccggcgat ctccctactc cggtgaaata
    20401 cttcaattcg caaatcgctc aggagtacaa ggctattgaa aaaatcgctc agcaaaaact
    20461 ggtcgatatg gttccggaag agctgcggga tatctttgcg ccgttaattg acgagcatgc
    20521 atatagcgat gaagaaaaat cgctggtgaa acaggcagat gcactgtgtg catatctgaa
    20581 atgtctggaa gaactcgcgg ccggaaataa tgaattcttg ctggcaaaaa cgcgactgga
    20641 agcgacgctt gaagcgcgtc gcagccagga gatggactac ttcatggaag tatttgttcc
    20701 cagcttccat ctttcgctcg atgagattag ccaggattca ccgctgtaag cagccggagt
    20761 ctgcgtcgca tcaggcaata agcgccggat gcgacatcag gctcttgtca aaacggaaac
    20821 agcatcggga tcatcaccac acaaaccgcc atcacgataa tggtgaacgg tacccccaac
    20881 ttcacaaagt cactgaagct gtaatttccc ggacctaaaa ccagtgtgtt aacaggtgaa
    20941 gaaaccggtg tcataaaggc ggcggatgct gccatcgcca cgaccatcgc gaatggataa
    21001 ggcgacaccc ccatcgtttt ggcagcagcc agcgcaatcg gagccatcaa caccgccgtc
    21061 gcggtattag agataaatag cccaataacc gccgacaaga caaacaaaca ccccagcatc
    21121 atatgtggcc cgtaaccacc gccaatgtcc atcagccctt tcaccgccag cgcgacacct
    21181 cccgttttct gtaatgccac agcaaacggc atcatcccaa cgatcaaaat aatgctcggc
    21241 cagtgaatgg atttataggc gctttcagca tctatacagc ggaatttccc catcagcagg
    21301 caggcgatga tagcggcaac aggattagga atttcatctg tcagcattaa cgccaccatt
    21361 agcaccagac agaaaatggc atggggtgcc tggctgtgcg cgggcgatgc ttcactcacc
    21421 tcttccggta agttcagcgc tacgaagtcg cggccctgtt tggccagcat accgatcagt
    21481 ttccagttac ccacaaccag gatgatatcg cccagcagca gaggctcatc cgccagcgaa
    21541 ccttccagcg ccacgccatt gcgctttagc cccaccacat tcagtccgta gcgggtacga
    21601 aaaccaattt cgcgcaccga tttaccaatc agttctgact caggaattaa tgaaatctct
    21661 gccatgccca catcaagggc ctggtcagaa aaatactcgc cgcgcagtac catcggctcc
    21721 agcaattgct cactacaaaa ttgccggaga tcgacatcag ccgcagacat atcaataagc
    21781 aaaacgtcac gcgcgcgaaa ttcagaaacc ccattaacgt tcacgataac gcgacgaaaa
    21841 cgccgccagc gttcaacacc gatgacgtta gcgccataac gctcacgtaa tttgagatca
    21901 tccagccgtt gaccaatcat tggcgatccg gggcgaatag ccagacgtcg cgcacgcccg
    21961 gtcagtcgat attcacggat aagatcgcga aaggttcgac gcgtccagcc ttcgcgctgc
    22021 ggggtctggg tatccccttt cagcatgaaa cgcatcacta acatatacaa aatacccagc
    22081 accagcacga ccaggccaat aggtgttacg ctaaagaaac tgaagccgtg atagccttca
    22141 cgcagcaatt cactgttgac taccaggttc ggcggcgtcg ccaccagcgt catcatgccg
    22201 cttatcagcc cggcaaaact caacggcatc atcagacgtg acggcgaagt ttgcatacgc
    22261 atggcaacgc ttaacaccac gggaataaag atagcgacaa cgccggttga actcataaac
    22321 gcgccaagcc ccgcgacggt cagcatcaac aaaaccaaca ttttgatttc actgttgccc
    22381 gcaactttga ccagccatgt tcccattacg gtggcaacac cggtacggac caaaccatcg
    22441 ccaataataa acaaggcggc aatcaggaca acgttaggat cagaaaagcc ggaaaatact
    22501 tcggggactg tcagcgttcc gcttaatgca aacgcgacaa taacaaacaa agcgaccgca
    22561 tccatacgca ctctgcccgt cgcaaacaag acaatggcaa cggccagtaa tgaaagaacc
    22621 caaatcaatt caccgttcac aacttatcct tgttaattga gggagatgtc ttgattctgc
    22681 cataaaaaag ccccgacgag acggggctaa atcatgatca ggtgtttcac tgaataataa
    22741 catcgccatt tggctgtttg gtcacagtaa tttgctccag actgtggagg accaaatcga
    22801 cctcattcag gcgcggggta tctgccggag cgttaaccgc aatgacatga caacccgccg
    22861 ccaggccaga aagcacgcca gcggagcatc ttccaccacc acacactcct gcggcgcaag
    22921 ccccagcagc tgcgcgccta acagatacgc atcaggttct ggttttccgc gcttcactcg
    22981 ctcagcggtt acaaacacct ctggtgcggg aagcccagct attttatggc gcgctcgcgc
    23041 taccggcatg gagccagaag tcacaatggc ccacggaata cctgctttat tcaaatgact
    23101 gagtaaggcg attgcccccg gaagcgggta ataccttcgg tttccgtggc ctcgatgtgc
    23161 tccagacgcg taaactcggc ggcaatatca gcctcggatt tgcccgccat aaaatggcgc
    23221 agagaggtga tcgcctgttt accgtgaatg aaagccagca cctcttccgg cgctaaccca
    23281 tgacgtctgg cccagttgct ccacgcccgt tctaccgcag gcagggaatc caccagcgtt
    23341 ccatcaagat caaacagaaa acctttgcac cgcacgcggg cctcctcagg cattgataat
    23401 ttgattaatt tcgttggcgc tcaaatggta ctgacgcggg caggcatgcc acacattaag
    23461 catgcgctga tatttttccc acattggcgt ctgggcgtta aaaccgtgag ttccggcgtc
    23521 aaaatgggta tagcgccctt ccacattaac cataaagcgg acataaccga ggtaacgtgc
    23581 ttcagtggcg gcgtcaaagc cgaggaaggt gacacgacgt tcatcgatgg attgctggtc
    23641 ctgcaaatta gaccaggaaa catgcaacgc atgatacatc tccataatgt cgatgatagt
    23701 gcggcaggtt tcttctttca gctcgccaaa ctcgcgatcc aattcacgca tctgtaatcc
    23761 gtaacctcgc tcaataattg tttgcaggcg acggtaacgt tcagcatttg ccggatcgag
    23821 catagtcatc atcttgtact gattagacaa aataagacgt tgcgcgttgg tcatttccat
    23881 tgttgactcc tgtatcactc tactacggtg aaaaaaaaga aggctgagta tgccttcttt
    23941 tatatgcgta atcaggggtc aattacaaat catcaaggaa agttttatcc agttgtttga
    24001 aggcgcgctt aagcgtgtca gctaatgcct ggtaatcagg cttgccttca acgggtgcca
    24061 acacctgtcc agactcctgc aatttaccgc gaacttcata aaaccagtta aggattgcag
    24121 ggggtaatgg cgttacagaa cgcttgccca gccaccacaa tccctgcatg ggtaaactta
    24181 aggcgaacag tgcagtggca actgccggcc caagctgacc gcccagggca atctgccagc
    24241 agagagtaaa tacggcgatc ggcggcataa aacggatcgc ataacgcgtc atcttgataa
    24301 cgcgattttc gacaaagacc ggggcaaggc gtttttccag tggccacgtc tttgagtaat
    24361 gctgtccgcg gcgaaacaag ctaaaaaaat taacagaacg attatccggc gttgacatgc
    24421 ttcacctcaa cttcacatat aaagattcaa aaatttgtgc aaattcacaa ctcagcggga
    24481 caacgttcaa aacattttgt cttccatacc cactatcagg tatcctttag cagcctgaag
    24541 gcctaagtag tacatattca ttgagtcgtc aaattcatat acattatgcc attggctgaa
    24601 aattacgcaa aatggcatag actcaagata tttcttccat catgcaaaaa aaatttgcag
    24661 tgcatgatgt taatcataaa tgtcggtgtc atcatgcgct acgctctatg gctccctgac
    24721 gtttttttag ccacgtatca attataggta cttccatgtc gagtaagtta gtactggttc
    24781 tgaactgcgg tagttcttca ctgaaatttg ccatcatcga tgcagtaaat ggtgaagagt
    24841 acctttctgg tttagccgaa tgtttccacc tgcctgaagc acgtatcaaa tggaaaatgg
    24901 acggcaataa acaggaagcg gctttaggtg caggcgccgc tcacagcgaa gcgctcaact
    24961 ttatcgttaa tactattctg gcacaaaaac cagaactgtc tgcgcagctg actgctatcg
    25021 gtcaccgtat cgtacacggc ggcgaaaagt ataccagctc cgtagtgatc gatgagtctg
    25081 ttattcaggg tatcaaagat gcagcttctt ttgcaccgct gcacaacccg gctcacctga
    25141 tcggtatcga agaagctctg aaatctttcc cacagctgaa agacaaaaac gttgctgtat
    25201 ttgacaccgc gttccaccag actatgccgg aagagtctta cctctacgcc ctgccttaca
    25261 acctgtacaa agagcacggc atccgtcgtt acggcgcgca cggcaccagc cacttctatg
    25321 taacccagga agcggcaaaa atgctgaaca aaccggtaga agaactgaac atcatcacct
    25381 gccacctggg caacggtggt tccgtttctg ctatccgcaa cggtaaatgc gttgacacct
    25441 ctatgggcct gaccccgctg gaaggtctgg tcatgggtac ccgttctggt gatatcgatc
    25501 cggcgatcat cttccacctg cacgacaccc tgggcatgag cgttgacgca atcaacaaac
    25561 tgctgaccaa agagtctggc ctgctgggtc tgaccgaagt gaccagcgac tgccgctatg
    25621 ttgaagacaa ctacgcgacg aaagaagacg cgaagcgcgc aatggacgtt tactgccacc
    25681 gcctggcgaa atacatcggt gcctacactg cgctgatgga tggtcgtctg gacgctgttg
    25741 tattcactgg tggtatcggt gaaaatgccg cgatggttcg tgaactgtct ctgggcaaac
    25801 tgggcgtgct gggctttgaa gttgatcatg aacgcaacct ggctgcacgt ttcggcaaat
    25861 ctggtttcat caacaaagaa ggtacccgtc ctgcggtggt tatcccaacc aacgaagaac
    25921 tggttatcgc gcaagacgcg agccgcctga ctgcctgatt tcacaccgcc agctcagctg
    25981 gcggtgctgt tttgtaaccc gccaaatcgg cggtaacgaa agaggataaa ccgtgtcccg
    26041 tattattatg ctgatcccta ccggaaccag cgtcggtctg accagcgtca gccttggcgt
    26101 gatccgtgca atggaacgca aaggcgttcg tctgagcgtt ttcaaaccta tcgctcagcc
    26161 gcgtaccggt ggcgatgcgc ccgatcagac tacgactatc gtgcgtgcga actcttccac
    26221 cacgacggcc gctgaaccgc tgaaaatgag ctacgttgaa ggtctgcttt ccagcaatca
    26281 gaaagatgtg ctgatggaag agatcgtcgc aaactaccac gctaacacca aagacgctga
    26341 agtcgttctg gttgaaggtc tggtcccgac acgtaagcac cagtttgccc agtcgctgaa
    26401 ctacgaaatc gctaaaacgc tgaatgcgga aatcgtcttc gttatgtctc agggcactga
    26461 caccccggaa cagctgaaag agcgtatcga actgacccgc aacagcttcg gcggtgccaa
    26521 aaacaccaac atcaccggcg ttatcgttaa caaactgaac gcaccggttg atgaacaggg
    26581 tcgtactcgc ccggatctgt ccgagatttt cgacgactct tccaaagcta aagtaaacaa
    26641 tgttgatccg gcgaagctgc aagaatccag ccctctgccg gttctcggcg ctgtgccgtg
    26701 gagctttgac ctgatcgcga ctcgtgcgat cgatatggct cgccacctga atgcgaccat
    26761 catcaacgaa ggcgacatca atactcgccg cgttaaatcc gtcactttct gcgcacgcag
    26821 cattccgcac atgctggagc acttccgtgc cggttctctg ctggtgactt ccgcagaccg
    26881 tcctgacgtg ctggtggccg cttgcctggc agccatgaac ggcgtagaaa tcggtgccct
    26941 gctgctgact ggcggctacg aaatggacgc gcgcatttct aaactgtgcg aacgtgcttt
    27001 cgctaccggc ctgccggtat ttatggtgaa caccaacacc tggcagacct ctctgagcct
    27061 gcagagcttc aacctggaag ttccggttga cgatcacgaa cgtatcgaga aagttcagga
    27121 atacgttgct aactacatca acgctgactg gatcgaatct ctgactgcca cttctgagcg
    27181 cagccgtcgt ctgtctccgc ctgcgttccg ttatcagctg actgaacttg cgcgcaaagc
    27241 gggcaaacgt atcgtactgc cggaaggtga cgaaccgcgt accgttaaag cagccgctat
    27301 ctgtgctgaa cgtggtatcg caacttgcgt actgctgggt aatccggcag agatcaaccg
    27361 tgttgcagcg tctcagggtg tagaactggg tgcagggatt gaaatcgttg atccagaagt
    27421 ggttcgcgaa agctatgttg gtcgtctggt cgaactgcgt aagaacaaag gcatgaccga
    27481 aaccgttgcc cgcgaacagc tggaagacaa cgtggtgctc ggtacgctga tgctggaaca
    27541 ggatgaagtt gatggtctgg tttccggtgc tgttcacact accgcaaaca ccatccgtcc
    27601 gccgctgcag ctgatcaaaa ctgcaccggg cagctccctg gtatcttccg tgttcttcat
    27661 gctgctgccg gaacaggttt acgtttacgg tgactgtgcg atcaacccgg atccgaccgc
    27721 tgaacagctg gcagaaatcg cgattcagtc cgctgattcc gctgcggcct tcggtatcga
    27781 accgcgcgtt gctatgctct cctactccac cggtacttct ggtgcaggta gcgacgtaga
    27841 aaaagttcgc gaagcaactc gtctggcgca ggaaaaacgt cctgacctga tgatcgacgg
    27901 tccgctgcag tacgacgctg cggtaatggc tgacgttgcg aaatccaaag cgccgaactc
    27961 tccggttgca ggtcgcgcta ccgtgtttat cttcccggat ctgaacaccg gtaacaccac
    28021 ctacaaagcg gtacagcgtt ctgccgacct gatctccatc gggccgatgc tgcagggtat
    28081 gcgcaagccg gttaacgacc tgtcccgtgg cgcactggtt gacgatatcg tctacaccat
    28141 cgcgctgact gcgattcagt ctgcacagca gcagtaacct cgtcatcatc cgcagctttg
    28201 cgctgcggat atctgaaccg gaaataatca ctatttccgg ttttttattc tcttaatctg
    28261 cattaatcct ttctgattat cttgcttaac tgcgctgcat caatgaattg cgccatttca
    28321 ctttacatac ttaccacttt gttttgtgca agggaatatt tgcgctatgt ccgcaatcac
    28381 tgaatccaaa ccaacaagaa gatgggcaat gcccgatacg ttggtgatta tcttttttgt
    28441 tgctatttta accagccttg ccacctgggt agttccggtg gggatgtttg acagtcagga
    28501 agtgcagtat caggttgatg gtcaaacaaa aacacgcaaa gtcgtagatc cacactcatt
    28561 tcgcattctg actaacgaag caggcgaacc tgagtatcac cgcgtacagc tgttcacgac
    28621 gggcgatgaa cgcccgggcc tgatgaactt cccgtttgaa ggattaacct caggatcgaa
    28681 atacgggaca gctgttggca tcatcatgtt tatgctggtg attggcggcg cgtttggcat
    28741 tgtgatgcgt acaggaacca ttgataacgg tatcctggcg cttattcgcc atacccgcgg
    28801 gaatgaaatt ctctttattc ctgcgctgtt tattctgttt tcacttggcg gcgcggtatt
    28861 tggtatggga gaagaggccg tcgcctttgc cattatcatc gcaccgctaa tggtccggct
    28921 gggctatgac agtattacca ccgtcctggt gacctatatt gccacgcaaa tcggttttgc
    28981 cagttcgtgg atgaacccgt tttgtgtggt cgttgctcag gggattgccg gcgttccggt
    29041 gctttctggc tccgggttgc gcatcgtggt gtgggttatc gccactatga ttggcctgat
    29101 ctttaccatg gtgtacgcct cacgagtgaa aaagaatcct cttctgtcac gcgtgcatga
    29161 gtccgaccgc ttctttcgtg aaaagcaggc ggatgttgaa caacgtccgt ttacctttgg
    29221 tgactggctg gtattgattg tcctgaccgc cgtaatggtc tgggtgattt ggggcgtgat
    29281 cgttaatgcc tggtttattc cagaaattgc cagccagttc ttcaccatgg gtctggtgat
    29341 tggcatcatc ggcgttgttt tccgccttaa cggcatgacg gttaatacca tggcttcatc
    29401 ctttaccgaa ggggcgcgaa tgatgatcgc ccctgccctg ctggtgggtt tcgccaaagg
    29461 gattttgctg ctggtcggta atggtgaagc gggtgatgcc agcgtgttaa ataccatcct
    29521 caacagcatt gccaatgcca ttagcggtct ggacaacgcg gtcgcggcct ggtttatgtt
    29581 gctcttccag gcggtattta atttcttcgt gacgtccggt tctggtcagg cggcgttaac
    29641 catgccgtta ctggcaccgc ttggcgatct ggtcggtgtt aaccgtcagg ttaccgtgct
    29701 ggcattccag tttggtgatg gcttcagtca catcatttac ccaacctcag cttcgttaat
    29761 ggcaactctc ggtgtgtgca gggtggactt ccgtaactgg ctgaaggtgg gcgcgaccct
    29821 acttggactg ctgtttatta tgtccagcgt cgtagtgatc ggcgctcagt tgatgggcta
    29881 ccactaaaaa tgttaagagc cgcaattgcg gctctttttt cattctgccg tttcagtctc
    29941 tacggcttca tttttggcat tgcgcttcat ccacaacgcc agcgctttca gcgagtctgg
    30001 agtgaactca tcgcagcgtg cggtgatttc ttccggcgtc agccagcaaa cttcactgac
    30061 ttcatcttcc tgtagggcga agggaccgtg agagacgcag ctgaacaacg cgccccagac
    30121 acggcaattt ttatcttcga aatagaactg cccgtgctcg gcaaagggga caccggcaat
    30181 gcccaactct tcttccgctt cgcgacgcgc ggattccagc agttgctcat cggcctgaac
    30241 cacaccgcct gcggtcgcat ctaacatgcc gggtaaaaag tcttttgtct cggtacgacg
    30301 ctggaccaga attttgccca tgccatcatg cacgacgatg taagttgcac gatgacgcag
    30361 acactgtgcc cgcatttgtt cccggctggc ttgtgcaatg acttcgttct cttcattgac
    30421 aatatccacc cattcagtac ttgccaaacg acgctgttcc attatcagga aaccttcttt
    30481 ttctggcgct cttacggcgc attttgaatt gtgggtaaat tacggattaa tcgcgacctg
    30541 cgcaatgata ctttgatcat tgagtgcgat tacactaagt acgtcattat ccagcatgcc
    30601 ataactcgcc ggattaccgc ctttcggaat actcaccgag ccggggttga agtggaaaat
    30661 ctccccccgc tgttctgcca ctggcagatg ggtatgaccg tacaccagca catcgttctg
    30721 gtttaaagca ggtagatttt ccgggccaaa aagatgacca tgcgtcaaaa acagacgttg
    30781 tttttccagt aatacctgtt gccacggcgc ggttatcggg aaatgcagca gcatttgatc
    30841 cacttcgctg tcgcagttgc cgcgcacagc gataacctta tgtgccactt cattaagccg
    30901 ttcagcgact ttggctggcg cgtaaccctc cggtaaagca ttacgcgggc cgtgattcaa
    30961 cacgtcgcca aggatcacca gccactgggc accgctttgg gcaaacaact ccagaacacg
    31021 ttccgtcgcc ggtaacgacc catgaatgtc cgatgcaaac atcagtttca tcactcactc
    31081 ctcgtcgaaa aaacgtaagc tatgatactg gattctgtcg ttgctatcag ccagattgct
    31141 tcgccgaaag tgcaataaaa cgctggacag acgctcgctg ccactggaat gtcgcataat
    31201 ccaccaggcg ttctggcacc tcatcgccat gtagtaccag gcggttaatc attagcgcca
    31261 gatcagtatc agcaatgcac cattcaccaa ataaattcgg ctgacccagt gctaacaaat
    31321 gttctgccat cgcgaacagt ttctctgcac tggcttttcc ctcggccgtt agtggcgctt
    31381 ttttcgcccc cgcaaagaca acatccgtcg gacgctcttc gcggatgggc atcagatcgc
    31441 tgcgcagcca ggcctgaatc cgtcgtgcac gcgcacgatt ttctaaatca agcggataaa
    31501 tacgttccca ggtcggtggc gcaaatcgat cttccagata ctccgcaatg gcagaagatt
    31561 cactcaactc aaaatcatcg atttgtaata acggcacacg gcgtgtctga ccgtaacctt
    31621 gccacgtcgg cttcaaatgt tcaccgctgt cgaggtcgat ggtcttgata tgaaacgaca
    31681 ggcctttttc ctgcaacgcc acccaggcgg ataacacata aggggagaaa aagtgggcat
    31741 ctgaccaaag cgtgatagcg ggtttactca ttatgtcctc ggcaataaag ggggctggct
    31801 atcaaactat agagcctttt tgtcactgtc acgtgacgaa tactcacgta agtcgccgcg
    31861 ctttctatac ttgtggtgtt cagatgatta aacaaccgga gctgcaatga tcgatctcta
    31921 tttcgccccg acacccaatg gtcacaaaat tacgctgttt ctcgaagaag cagagcttga
    31981 ttatcgcttg attaaggtag acctggggaa aggcggtcag tttcgtccgg aatttttgcg
    32041 catttcgccc aacaacaaaa ttccggcaat tgttgatcat tctcctgccg atggcggcga
    32101 accgctaagc ctctttgagt ctggtgccat tttgttgtat ctggctgaga aaacaggact
    32161 ctttttgagt catgaaacgc gtgagcgcgc cgccacatta cagtggttat tctggcaggt
    32221 aggcggactg gggccgatgc ttgggcaaaa tcatcatttt aatcacgcag ccccccaaac
    32281 cattccttac gctattgaac gttatcaggt tgaaactcag cgtctttacc atgtactaaa
    32341 caagcggctg gaaaactcgc cctggctggg aggcgagaac tacagcattg cggatattgc
    32401 ctgctggccg tgggttaatg cctggactcg ccagcgaatt gacctcgcaa tgtatccggc
    32461 agtcaagaac tggcatgagc ggatccgttc gcgccctgcc accgggcagg cactgctaaa
    32521 agcacaactc ggtgatgagc gttcggatag ttaacagaaa caggttctcg tgtattattt
    32581 catcctaagt aaaacaacgg agaacctgca atggcacaac ctgccgctat tattcgtata
    32641 aagaaccttc gtttacgtac gtttatcgga attaaggaag aagaaattaa caaccgtcag
    32701 gatattgtta tcaatgtgac gatccactac cccgccgata aagcgcgcac tagcgaagat
    32761 atcaacgatg cgctgaatta tcgcaccgta acgaaaaaca ttattcagca tgtagagaat
    32821 aaccgtttct ctttgttgga aaaattaact caggatgtgc tcgatatcgc acgtgaacat
    32881 cactgggtga cgtatgctga agtggagatc gataaactgc acgcgctgcg ctacgccgat
    32941 tcggtatcca tgaccttaag ctggcagcgt taaccgccat atcgggaggc tgcatgaaca
    33001 tagtgatcac cggagggacg ggattaattg gtcgccattt gattccgcgt ttgctggagc
    33061 tgggccatca aatcacggta gtgacgcgta acccacagaa agccagttcc gttctcggcc
    33121 ctcgggtgac gctatggcaa gggctagccg atcaaagcaa cctcaacggc gttgatgcgg
    33181 taatcaacct ggccggagaa ccgattgctg ataaacgctg gactcacgag caaaaagagc
    33241 gtctctgcca aagccgctgg aatatcacgc aaaaactggt cgatttgatt aatgccagcg
    33301 acacgccgcc gtcggtactc atttccggct cggcaacggg ctattatggc gacttaggtg
    33361 aagtggtggt taccgaagag gaaccgccgc ataacgaatt tactcataaa ctctgcgccc
    33421 gctgggaaga gattgcctgc cgggcgcaaa gtgacaaaac gcgagtgtgc ctgctgcgaa
    33481 ccggcgtggt actggcaccg gacggcggta ttctcggtaa aatgctgcca ccgtttcgtc
    33541 ttggcctggg cgggccgatt ggttccggtc ggcagtatct ggcctggatt catattgatg
    33601 atatggtcaa cggcattctc tggctgctgg ataacgacct gcgcgggcca tttaatatgg
    33661 tttcgcccta cccggtacgc aatgaacaat ttgcccatgc gctcggtcaa gcgctgcatc
    33721 gcccggccat tttgcgcgtc cccgcaaccg ccattcggct gttaatgggc gaatcttcaa
    33781 tactggtatt aggtggacaa cgcgcgctgc ctaaacggct ggaagaagcg ggttttgcgt
    33841 ttcgctggta cgatttagaa gaggcgctgg cggatgtcgt tcgctgatgt ggtttacagc
    33901 aaacatccgc cagttaactc ccggtgttac aggattagtg gctttgcgcg ataagatcgt
    33961 ctggtgaaag tcgggtcacc atcataacta actctctgtc taaacctcta tccagcatct
    34021 cctgagcaat acgcagggct tcttcgtgtt tgccctgcat tgcgccttct tcacgtaatc
    34081 tgtcagcaat ggtcatcagt ttctcctttt cttgtggtgc gcgttctgtt atctcaccaa
    34141 taaatgcacg gaaacgctgg gcatcccctg tttgtaatac gtaattaaac agggctttta
    34201 gctgtctgtc attagtgttc cctgtaacta gcagcgaaac aatttggtcg actaatccca
    34261 acagatcgcg ctgacgaata tgtttctgaa ttaactccaa cagcgccatt ttgcggtgtt
    34321 gcataatctc gtcatccggc accacggtaa tatccaccaa cggaaacgct gatgaatata
    34381 ttttgcgggc tatagcaggc tcggcaaatt catcaagcca gcagagtgaa taaggataag
    34441 gacttctgca accatgataa aacagcatcg ggatcaccaa tggaagctct ttatagcccg
    34501 catcaagatg gttttgcatt gccgcaatgg aataacgcat catgcgaaaa gccattaatt
    34561 cttccagctt actttggtgc tctatcacta cataaatata acccactccc tcctgcgttt
    34621 tcacagacca caagaggtcg gaataatatt gccgcaggtc ttcatcaata aaactgtttg
    34681 attctagttt aagcgtcgtt aaatcacaca gtttgcgcag cggcgcggga agatgaatat
    34741 caataaaatc ccgcgcggtg tctggatggc gtaaaaaaga tttaaatacc gcatcatgcg
    34801 gcgtggaagt tgtcgatatt gtcatggcgt tccgtcaccc ctcaaattta agatgacgcg
    34861 acaataacca cgattaacac ccaatgcagc aggcagtttt tatctttgga cagcgaattc
    34921 agaagtattt ttagcggact gcaactttta catttcagcg gaagctgact cgcaaattcc
    34981 ccatgaaaat actccggcag cgttagccac cggagaggaa attatttcag cgatccctta
    35041 aggaactgtt gcagacgagg gctttgcggg ttgccaaata actgctccgg cgcgccctct
    35101 tcttctattt tcccctgatg gaggaaaatg acgtgagtag aaacatggcg agcaaagccc
    35161 atttcgtgag tcactaccac catggttttc ccctcttctg ccagttgctg cataatacgt
    35221 aacacttcgc ctaccagttc aggatcgagc gccgaggtag gttcatcaaa cagtaaaact
    35281 tccggttcca tcgccagcgc ccgcgcgata gaaacacgct gttgctgacc gccggaaaga
    35341 tgcaccggat atttcccctg cgcacgttcg tctatcccga ctttcgccag atacttcacc
    35401 gcccgctcgc gcgcttcctg cttgctcagg cccaacacct gaatcggcgc ttccatgacg
    35461 ttttccagca ccgtcatatg gctccagaga ttgaagtgct ggaataccat cgtcaggcgt
    35521 gtgcgcagta agcgcagttg atttttatcg gcgactttga gttgaccgtc tttgtcgcgc
    35581 accagattga tcgtctggcc attgaccacg atcgaccctt cactcggttt ttcgaggaag
    35641 ttaatgcagc gcagaaaggt acttttcccc gatcccgacg atccgatgat gcttattaca
    35701 tctccggcat tcgcttgcag tgagacccct ttcagcactt catgttcgcc gtagcgtttg
    35761 tgcaaatcga taacgtttaa tttattctcg gacatcgtat tctcagtgcg ttgaagaagg
    35821 tttcacatgc tgcaaccagc gtttttccgc tctgcgaaag aggctgatca ggacataaga
    35881 gatgattaaa tagagcaccg cggcaatgcc gaaggcggta aaaggttgat acgtggcggc
    35941 gttaatatcg cgggctattt tcagcagatc cggcacagtg gccgtaaacg ccagcgcagt
    36001 agagtgcagc atcaggatca cttcgttgct gtatgccggt aacgcaatac gcagcgcaga
    36061 aggcaaaata atgcaacgat acattttaaa agtcgagaag ccataggctc tggcggcttc
    36121 aatttcccca tgcggaaccg aacggattgc cccagcaaaa atctcggtag tgtaagcgca
    36181 ggtgttaagc gtcagcgcca gcacggtaca gttcaggcca ctgcggaaga aagcgttaag
    36241 gaattcggtt cccttaacaa tctcaagcgt gtacatgccg gaatagaaca ccagcaactg
    36301 aacatacagc ggcgtaccgc gaaaaatata ggtaaataac cagattggaa actggatgta
    36361 tttattactg gagacacgac caatcgccag aaacagcgcc aggactccgc ctatcactac
    36421 cgacaaaata agcagccaca gagtgatcgc cacaccagta aagcgataac cgtcggtcca
    36481 cagcagcggt ttccagtatt catgtaagat ttcgatcaca ggtcagccct cttcacaccc
    36541 acggagtagc ggcgctcaag gaacagcagc acaccattgg aaacagtggt gaaaaccagg
    36601 taaatcacgc cacagacgat ggcgaaatag aacggttccc aggtactttt gcctgccagt
    36661 tgggtggctt tgaccacatc ttccaggccg agtaacgaaa ccagtgcggt agatttgagg
    36721 atcacctgcc agttgttgcc aatgcctggc agggcgtaac gcatcatcgc cggaaacatg
    36781 atccgccgaa acacttgccc acgagtaaaa ccgaacgccg tcgccgcctc tatatgtcct
    36841 ttcggcactg ccataaaagc accacggaac gtttcggtaa aataagcacc gtagataaaa
    36901 ccgagagtga taataccagc gaccatcgga tcgatatcaa tctgcccgac gcccatcgcc
    36961 tccgtcaccg tgtttagcgc aatctgcaaa ccgtagaaaa tcagcagcat caacactaag
    37021 tccggcacgc cacgaatcag cgtggtgtac ccttcgaaaa tcagccccga aagccgattt
    37081 tgcgagagct taccgccagc gccaattaaa ccgatgatta cagcgagcac tacagagctg
    37141 atagccagct ccagcgtgac gagcgcaccc tgtaaaataa cacctgaaaa cccatacaac
    37201 atgctgcctg tcctgtcgtg tgtggtgaat taccgcctct tgtctccctc cggttacccg
    37261 aagggagcgg gctttctttc accgatggcg attagccacc ataaacatca aaatcgaagt
    37321 actttttcgc taatttctcg taagtaccgt cagcgcgcat ttcggcaaag gctttgttca
    37381 gtgcttcgcg cagttcgtta tcttctttac gcaggcccat gccggtccct acgccaaaca
    37441 gtttttcatc tttaacagac gggccaccga atttgtaatc tttaccgaca ggttgtttga
    37501 ggaaaccttc gctggcagcg acctcatcct ggaacgcggc atcaatacgt ccggcagtca
    37561 ggtcagaata aatgttgtcc tgcccctgat acgagacgat ttcaatgcct tttggtgccc
    37621 aatgttcatt accgaacgtc tcctgggtgg tgccctgcaa tacgcctacc cgtttgcctt
    37681 tcagcgactc gactgtcggc tgaatgtcag aatttttcgc caccaccaaa cgagaatcgg
    37741 cagcgtacag tttgtcggtg aaggctattt cttgctgacg tttttccgta atggaaagcg
    37801 atgacatgat ggcgtcaatc ttcttcgctt ttaaggacgg gattaacgca tccagcggat
    37861 tttcgacaaa cgtacattgc gtattgatgc gtttgcataa ttcctttgcc agatcgatat
    37921 cgaagccaac cagttcgcct tgtgaatttt ttgattcaaa tggcgcatag gtcgggtcgg
    37981 taccgatgcg gatgttttgc ggaatcgcag caaatgccgc agttgcgctg gagaaggcca
    38041 gaaccagaga gagcgatagc accagttttt tcataatgtc ctcaactgac agtcttttta
    38101 tagggaatat ttacaggttt gttacactta tcgtgccata aaattgacca tcaaggcaaa
    38161 atattccgcc ccgttagacg tattattttt cattaaaaat gcttaagtat gcatttcaat
    38221 gcaccacgat agtgcaacaa acctggcgtg caccatgatg atcattccat caggtacagc
    38281 ttcccagcga cgtatcagtc accgtagaca ttaaagtcga aatacttttt cgccatcttg
    38341 tcgtaggtgc cgtcctgacg cagctcgcca agcgccttat tgaaggcagc cgtcagttca
    38401 gcatcatctt tacgtagccc tacaccggtg ccatcaccga agtatttttt gtcttttact
    38461 gatgagccag caaaggcgaa agctttacca gcaggttgct tgaggaatcc ttcgctggca
    38521 gcaacttcat cttgtaacgc agcatccaga cgtcctgcag ccagatcgga atagaccaaa
    38581 tcctggttgg cataggccac cacatccacg cctttgctac gccaggtctc gttagcgtaa
    38641 gcttcctggg ttgatccctg cagcacacca acatgtttac ctttcagtga atccagcgtt
    38701 ggctgaatcg gtgaaccttt ggcagcaatc aaacgagaat ctgcggcgta cagcttgtcg
    38761 gagaaggcaa tctcctgctg acgtttatcg gtaatggaaa gcgacgaaat aatggcgtcg
    38821 atttttttcg ctttcagtga ggggatcagc gcgtcaaagt cactggcaac ccaggtacat
    38881 ttcacctgca tccgtttgca catctcgtta ccgagatcga tatcaaagcc aacaaaatca
    38941 cctttagcat ctttcgacga gaacggcgca taggtggtat cggttccgat acgtaccgtc
    39001 tccggtagcg ccgcatagct ggaagccgct gcggagagac cgactaacaa agacagagcg
    39061 agaatcgact tcttcataca taaccctcaa gtgaaaatgg cttttttatg ttgtgtattg
    39121 tgttgtgtgt ttgcaggctc tttcatgcag gtcttatgcc atcttgacga caacagtaac
    39181 attcaacgtt aaatatgtta ataagacgtt gcattattgt cctgaagttg aagatagcag
    39241 gtatagcggt tgaatcgcag cgtttcgatt gaatggcaga aacaaaatgt cgaggatttg
    39301 atcgcggttg caaaattgcc ctgaaacagg gcaacagcgg agttatgcgc cctgccagcg
    39361 ggcaaagaga tcttcaggaa gggttattgc aaactggtca agaacacgat taaccgtctg
    39421 atttatcaca tcatcaaggg attgcgggcg atgataaaac gccggaacgg gaggcataat
    39481 caccgcaccg atttctgccg cctgagtcat taaacgcaga tggcctaagt gcaatggtgt
    39541 ttcacgcacg cagagcacca acgggcgacg ctctttcagc accacatctg ccgcacgggt
    39601 cagtaagcca tcagtatagc tatggacaat gccggaaagg gttttgattg aacagggtaa
    39661 aatcaccatc cccagcgtct ggaaagaacc ggaagagatg ctggcggaaa tatcgcgcgc
    39721 atcgtgcgtg acatcagcta atgcctgcac ttcgcgcagg gaaaaatccg tttcgaggga
    39781 taaggtctgg cgcgccgcct ggctcatcac cagatgcgtt tcgatatctg tgacatcgcg
    39841 cagaacctgt aataagcgca cgccataaat cgcgccgctg gcaccgctga tgcctacaat
    39901 gagtcgtttc ataaatagtt gccctgtcag acttgcaggc agactttgca gtatttcggc
    39961 gggagttgca agtcagggtg ccagaccggc accctcagcg aaggcatcat ccttcgttat
    40021 gcatttcgag attttccact tcgttctgac gttgcactgc tttggcgtca tcattacgta
    40081 acgtatcgag gaaatcgagg tagccctgat caacatcttt ggtgacgtag acgccgttga
    40141 acaccgagca ttcaaactgc tggatatccg gattttcagc gcgaacggcg tcgatcagat
    40201 cgttcagatc ctggaaaatc aacccgtcag caccgatgat ctggcgaatt tcatcaactt
    40261 cgcgaccgtg agcgatcagt tccgtggcgc tcggcatatc aataccataa acgttcggga
    40321 agcgaatttc cggtgccgca gaagcgaggt acactttctt cgctccggct tcgcgtgcca
    40381 tctcgataat ctgctcagaa gtggtgccac ggacgatgga gtcgtcgacc agcaggacgt
    40441 ttttatcgcg gaactcggcg cggttggcat tcagtttacg gcgcacggac ttacgacgca
    40501 gctgctggcc cggcatgata aaggtgcggc caacatagcg gtttttaacg aagccctggc
    40561 ggtacggttt gcccagaata cgagcaattt ccagcgcgat atcacacgag gtttctggga
    40621 tcgggatcac cacgtcgata tccagatctt cccattcgcg ggcaattttc tcgcccagtt
    40681 tcgtgcccat attcacacgc gcgctgtaaa cggaaatttt gtcgatgaac gagtccgggc
    40741 gagcaaagta tacatactca aacaggcacg gattgctgac cggattgtca gcacattgac
    40801 gggtaaacaa ctgcccttct tcagtgatgt aaatcgcttc gcccggcgcg acgtcacgca
    40861 ggaaatcaaa gcccagcgta tcgagcgcta cgctttcgga agcgaccata tattctgtac
    40921 ggttctcgtc aatatcacgt tttcccagta ccagcggacg aatcccgttt ggatcgcgga
    40981 aagcaaccat accgtggccg ataatcatcg ccacacaggc atatgcgccg cggattaagc
    41041 ggtttgtggc agcaatggca gcgaaaatat tgtcggcttc cagcgggtag tggcggaagt
    41101 tgtccagctc gctagcgaag atattaagca gaatttccga gtcggaagtg gtgttgatgt
    41161 ggcggcgttt ttcttcaaac agttttttac gcaactcgtg agcgttggtc agattgccgt
    41221 tgtgggcaag cgtaatgcca tacggggagt taacgtaaaa cggctgcgct tcagaggcgc
    41281 tggagctgcc agccgtaggg taacgcacat gaccaatgcc catattgccc tgcaaacgct
    41341 gcatatggcg agcttcaaat acatcgctca ccagcccgtt cgctttacgc aaacggaagc
    41401 agttattggc atctatggtg atgatgccgg cggcatcctg accgcgatgc tgaagcaccg
    41461 ttaaggcatc ataaatcgac tggttaaccg gcataacacc ggcgataccg acaataccgc
    41521 acatacgtct ttttcctcgt taagccacat ctcagagcac ttacgctctg ggcaagaaac
    41581 ttgacgagct ttgcagataa tcaaaaaacc atctgatgat aaaactgaac tgcgggatca
    41641 gctgtgattt gctccagtct tcgcttttcg acaccccggt aaaggagtcg agaaagaaga
    41701 gaatggcggc aacaatcaac acaccgcgca acgcaccgaa acagacgccc agcacccgat
    41761 cggtgcctga caaccccgtt ttctccacca actggcctat cacgaagttc acgatagcac
    41821 caacaatcag ggtagcgata aacagtaccg cgatggcaat tccatttcga accagttcgt
    41881 cttcaaagcc cgtaaaccag actgacaggt aagtgtagta atgactggca acaaagaaag
    41941 cacaacccca tgtcaccagc gataacgctt cacgaacaaa gccgcggatc aggctaacca
    42001 gagaggaaaa agcaatcacc gcgattatgg cgtaatcaat ccagaccata tgtgtcccac
    42061 gattttacgc cctgtcatcc tgttcggggc gcattctaac agaaaaagaa aacgtttgcg
    42121 tagggatttc cttcccacgc atcaataaaa atggcgctga aaaaatattc aacgccatcg
    42181 actttttatg cctttgcggc atcgtgcaat gcgtgtcgga tgcggcgtaa acgccttatc
    42241 cgacctacgg ttctaacact gcccaggcct gataagacgc gccagcgtcg catcaggcaa
    42301 gactgtatta attcggcgta tagcccatta ccacgccact taagccagaa agttgcttca
    42361 actcacccag cgaacctttc agcttatctt tcgaggcatc cggcccaacc agaatacggg
    42421 taattttacc ctgcactggc gtggatggcg acgtataaac ccgataaccg gcaccgcgca
    42481 gcttaccgac aatctcattc actttatcgg catttttcag cgcacccagt tgcacaacat
    42541 aggctttacc cgttggcgct gctttttctt ccacgaccgg cttcggttct ggcttcggcg
    42601 caggtggtgc ttcaaccttc ggtttaggcg gctccaccgg tttcggcttc ggtggggcga
    42661 ccggtgcagg ttccggttca aactcggtgt tattggctgc aatagtggcc ggatcgagcg
    42721 acggtgctgc ggcatcacct gcccgcacct cttccgctgc gccttccggc ggctgcgtcg
    42781 gcaacgcctg ggtggcggct ggcatcatat caggctcatc gcgatcgccc gcttttggaa
    42841 ccagcgggat agccgcaaac tcatcctgat aatgtttttt ctgcccgtcc agcagccctg
    42901 gaagtacaat cacccccagc gccaccagca cgatcgtgcc cactaaccga ttctgaaact
    42961 tacttgccac cgcttctcct cgcgtcaatc acttccatga catgtgcgac cgtgtggaaa
    43021 gaaccacaca ccagcacggt gtcttccgct ttagcgtccg ccattgcggc atcccatgcc
    43081 tgcgcaacgc tatcaaatga tttgccgtta cccaaatgct caagcagttg ttctgccgtg
    43141 gcaccgcgcg gcccttccag tggcgcacaa taccagtcat caaccacgct tttcaaccag
    43201 gccagagttc cggcaatatc tttatcatgt agcataccga taaccgccag cacgcgcccg
    43261 tttttcggta gcgctttcat acgcccggtg agatattccg ccgcatgtgg attatgcgcg
    43321 acatcaaaaa taacgcgtgg cgactcgctc acaatctgga aacgtcccgg caaaattgcg
    43381 ctggcaatcc cgtcgcgaat ggcattttca ctgacttcca gcccgctggc acgcagtgcc
    43441 gccagcgctg ttgcggcatt cggttgcggg acaagcggca acggcagatt ttccagcgtg
    43501 ccgtgagcat cgctaaacgc ccagtcatga tcggtgacgg aatagttcca ctcaacgccc
    43561 cgacgttgta acagtgcacc tttttcctgc gccacatcag caatggtaga aggcatttcc
    43621 ggctcaccga caattgccgg ttttgcgctg cgaaagatgc ctgctttctc gcgaccaata
    43681 ctttcgcgat ctggtcccag ccagtcggta tgatccagcg caatgctggt tactacagcc
    43741 acatcggcat cgacaatatt ggttgcgtcc agacgaccgc ccagccctac ttccagaatc
    43801 actacgtcaa gttgtgcctg tttgaacagc cacaacgccg acagcgtacc gtactcgaaa
    43861 taggtcaggg aaatatcacc gcgtgccgat tcaatctccg caaaagaggc ggtgtgggcc
    43921 gattccggca attcctgccc ctgcacacgt acgcgctcgg tataacgcac cagatgcggt
    43981 gaactgtaga cgcccacttt gtaccctgcc gccatcagaa tcgactccag cgtacggcag
    44041 gtggtgcctt tgccattcgt acccgcaacg gtaaacacaa atggcgctgg tttcaggacg
    44101 ccaagacgcg ccgcgacctg gctcacgcgc tcaaggccga gatcgatagt tttactgtgc
    44161 aggttttcca gataagaaag ccacgaagcc agaggcgacg cggcttgagg agtgcgtttg
    44221 ataatcatgg tatccgctga ttcgttacgg tgagaatagc aaaagggcag agccagtggc
    44281 cctgccctta tcagttatca ggcctcaggt tcctgatccg gtaccggggg taccactacg
    44341 ccttcacgcg gcgcttcagg attcggcgct ggcagattca tcaacttcgc cagaatgctc
    44401 gccagtttca ggcgcatttc cggacgatgg acgatcatgt cgatggcgcc tttctcgatc
    44461 aggaattcac tgcgctggaa tcctggcggc agtttttcgc gaacggtctg ttcgataaca
    44521 cgcggaccgg caaagccaat taacgctttc ggttcagcga tgttgagatc gcccagcatg
    44581 gcgaaacttg cagaaacacc gcccatcgtc gggtcggtca gcacggagat gtacggcaag
    44641 ccgcgctcct gcatttttgc cagtgccgca gaggttttcg ccatctgcat cagcgacatc
    44701 agtgcttcct gcatacgtgc gccaccagag gcggagaagc agatcagcgg gcagttatct
    44761 tccagcgcct gctcaacggc acgcacgaaa cgtgcaccca caacagaccc cattgaaccg
    44821 cccataaagg cgaactcgaa tgccgcagcg acaaccggca ttccatacag agtgcctttc
    44881 atcaccacca gcgcatcttt ttcgccggtt tctttctgcg cagaagccag acggtcttta
    44941 tacttcttgg agtcacgaaa cttcagcaca tctttcggct caagctcgct acccagctcc
    45001 acaaggcttc cttcatctaa caggctatgc aggcgattac gcgctgtcat acgcatgtga
    45061 tggtcacact tcggacagac ctcaagatta cgttccagct cagcgcggta taaaacctga
    45121 ccgcagctat cacacttagt ccacacccct tcaggaatgc tcgccttgcg ggtgggagta
    45181 atgttgcttt taattcgttc aatccagctc attagggacc tttctgtctg aacctggttc
    45241 gatgccagtt ttatctttgg ggacgcataa tgccattttt gcccccaaca gaccatgaat
    45301 gttgcacatt aaaacataac agcccgaaac tttggataaa aaagtggtcg aaccgcggag
    45361 ttacttttta ttttgcggcg cgtgccgcag cgcgtttgtg acgaataaat tcgaaaaccc
    45421 caggcaatat tgaaacaaaa ataatgccaa caatcatcag cttaaggttg ctctgaatga
    45481 aggggagcgt gccgaagaaa taacccgcgt aggtaaaaat cagtacccac aacagtgcgc
    45541 cgatcacgtt ataagcggcg aaatgacggt acgacatgtg gcccattccc gcaacaaacg
    45601 gcgcgaacgt tctgacgatc ggtacaaaac gggcgagaat aatcgttttg ccgccatgtc
    45661 tctcgtaaaa ctgatgagtc ttatcaagat aactgcgacg aaagattttc gaatccgggt
    45721 tactgaacag tttgtcacca aataaccgtc caatggtata gtttactgca tcaccgacaa
    45781 tagcggcaat caacataagc accaccatca tatggacatt gagatcgtta gtttccagcg
    45841 acgccaatgc accagcaaca aacagcagcg aatcaccagg caggaatggc gttaccacca
    45901 gccccgtttc gcagaacaga atcaaaaaca gaatggcata aacccagacg ccgtactccg
    45961 cgaccagctc tgccagatgc acgtcaatgt gcaggataaa atcgatgagg aaataaatca
    46021 ggtccatatt tgcctatgcc ttgtactcgt catctttcag gctgtaacta cgttggctac
    46081 agcctgaaat gttccgagca ttattctcgt tagtccgcca gaaatagcgg gcccattggc
    46141 ggttttggaa gatcataccg gtcagggtaa tccaccgcga ccagatacag cccttccgct
    46201 tttgccgttg ctgccgccag cgttctgtcc tttgccgcca gcaactctgc tatccatctc
    46261 tccggctggt tgtgggcacc gacttccatc aggctgccga caatattcct gaccatatga
    46321 tgtacaaagg cattcgcttt gatatctacc accacataag gaccgtgacg cgtgacatta
    46381 atatgcataa cgttgcgcca cggggttcgg gactggcact gcaccgcacg gaacgaggtg
    46441 aaatcattct cgcccagcaa gcattgcgca gcccgatgca tccgttcagc gtccagcggt
    46501 tcgtaaaaat gggttacccc tttactcaat accgccgggc gcagccgatg attgtagatg
    46561 atgtagcgat aacggcgagc cgtggcgcta aatcgggcat gaaaatcatc aggtacagct
    46621 ttaacccaac gcacggcgat gtcaccaggt aaattcgcat ttacgcccaa ggtccacgcc
    46681 gcgtctttgc gctgcgcggt tgtttcgaaa tgcaccacct gcccggtacc gtgtacccct
    46741 gcgtcagtac gcccggcgca gaagacggtg atgggttcgt tcgccacctg ggagagcgcc
    46801 ttttccagct tctcctgcac actgcggact tcattctgcc gttgccagcc gtaatactta
    46861 ctgccgtcgt actcaatgcc cagcgcaatt ttataaactg gcagttgttg ctggtcggac
    46921 attagtacag atactcctgc accagtttct cggcgatttt tactgccatc agcgcgccgc
    46981 caaagcgaac gttatcggcc accgaccaga actggacttg ctccggcata ccgtagtcat
    47041 tacgcacgca gccaacagaa agatgcggcg tacccgaagc atcccctacc tgagtcggga
    47101 attcgttctc ttcagagagc acaatatctt cgccctgagc aaacgcatca cgcgcttctt
    47161 ctgccgccag cggacgcagt gcttcaaagt tgaccatctg ggcatgaccg tagaataccg
    47221 gtgcctggac gacgcttgcc gaaatcatca gcccttcgtc ctgcaggatt ttgcgtactt
    47281 cgtcaacgat acgacgttct tcacgcacgc taccttcgct atccggcaac aacggcagca
    47341 tattgaacgc cagttgacga ccgaagaaat cttcttcgtc aatcggaatg ccgttgagca
    47401 atttcgcact ctgccccgct aacgcatcga ccgctttttt gccctgggcg gaggctgaaa
    47461 tcaggctggt aacactgata cgcgacaaac cgccctgatc gattaacggt ttcagtgccg
    47521 ccagcagctg gctggtcaga ctgtctggta cggcgatgac attccggttg cggtaatcgg
    47581 tcagtacaaa cgggtttact tccggcacca ccagcggtac gtcgggttcg agagcaaaca
    47641 gtccgctgct gtcgatcacc aggcaacctg agttggtcgc ttcttcaacc caggcagcgg
    47701 tagcttcttt gcctgcgaca aaaaatgcca gctgcgcctg cgtccagtcg aattcagcgg
    47761 catcctgcac agtgattgtt ttaccaccaa agcgcagttg ttcgcctgcg ctttcgttac
    47821 gtgccagtgc ataaatttcc ccaaccggga actgacgttc agccagcgtt tcaagcaggg
    47881 cttcgcccac agcgccagtt gcgcccagga cggcaatgtt ccagccttca gacatggtgg
    47941 tttactccag aaatagcaaa gctccctgcc aatgttacag cagagagcat gaagaagaga
    48001 ttaacgtgcc ggatgatgaa cggcgttaaa acccagttta cacagcaatg atgccgcact
    48061 ggcgtcatca caaattacat acagagatga ccattcacgg cgctcaagat agtttttgcg
    48121 cagtttatcg aactcacccg gtatcccggc gactttacgc agcggtgcgt catcgcggcg
    48181 cacatcatac accaaatgca ccagcctttt cagcgtcggt tgatcgagcg ggccatgcag
    48241 cgtaatgcga ccaaactctg gcgcgggcag taatgtatcc agcgcaacgt gctgttcatg
    48301 cccaataaac ttgctatagg cttcaaacac ttgcgtagta ccgcgtgctt taccttccag
    48361 ggtataacct gcgatatgcg gcgtgccgat atccactttt gtcagcaact ccacgttaag
    48421 ttccggttcg ccttcccaga catccagcac tacgcttaac ttctggcctt cattcaggca
    48481 ggtcagcaac gcagtattat cgacgactgc gccacggcag gcgttaatca gaatcgctcc
    48541 cggcttcagg ctacggatca gtttttcatc cgccagatgc agcgttttgt acggcccatc
    48601 tttgaagagt ggcgtatgga aagtcagaat atccgcatgc tgaactaatt catccagcga
    48661 gcggaaatca ccctcatccc cacggtcagc gcgaggcgga tcgcaaagca atgttttaat
    48721 ccctaacgct tccagtcgcg cctgcaatcg acgtccaacg ttaccaacgc ccacgatccc
    48781 caccgtacgg tcgtgcagtg aaaatccatc gcgttcggca agcatcagta gtgaggagaa
    48841 aacatattcc accaccgcaa tcgcattaca gccaggtgca gcggaaaaac caattcccgc
    48901 ctgcttcaac catgcttcat cgacatggtc ggtccccgca gtggcagtgc caacaaattt
    48961 aatgggtttt cctgccagca aagattcatt cactttcgtg accgaacgca ccatcagcgc
    49021 atccgcgtct gccagttgag cgacggggat tggacgcccg ggaaccgcgg tcacctcacc
    49081 caaacggcta aataagtcgc gggcataagg catattttca tcaacaagga ttttcacgtt
    49141 tgtgttacct gtatgagacg agagttaacc ggacaagtgt gccataatct cgcggccagg
    49201 catacttgcg aagatttcag gtataaggat acgtaatgat acaacctatt tccggccctc
    49261 ctcctgggca accaccaggt cagggagata acctgccgtc tggcgcgggc aatcagcctt
    49321 tatccagtca gcaacgtact tcgctggaaa gcttaatgac gaaagtgacc tcactgacgc
    49381 aacaacaaag agcagaactg tgggcgggta tcaggcacga tattggtctg tcgggagatt
    49441 caccgctgct ttcgcgtcac ttccctgccg ctgagcataa tctggcgcaa cgtctgctgg
    49501 ccgcgcaaaa aagccattct gcccgccagc ttttagcgca attaggggag tatttacgtc
    49561 tggggaataa tcgtcaggcg gtcacggatt atatccgtca taactttggt cagacgccgc
    49621 tgaatcagct ctcaccggag caattaaaaa ccattctcac cctgttgcag gaagggaaga
    49681 tggttattcc gcaaccacag cagcgcgagg cgaccgaccg tcctttatta ccggcggagc
    49741 acaatgcgct aaaacagctg gtgaccaaac ttgcggcggc aacgggggaa cccagcaaac
    49801 agatctggca atcgatgctg gaactttccg gggtgaacga tggcgagtta attccagcga
    49861 aactgtttaa ccatctggtg acctggttgc aggcgcgcca gacgttaagc cagcaaaata
    49921 cgccgacgct ggaatcacta cagatggcgc taaaacaacc tttagatgcc agtgaactgg
    49981 cagcgttatc ggcgtatatc cagcaaaaat atggtctttc tgcgcaatca tcgctttctt
    50041 ctgcccaggc cgaggatatt cttaatcagc tttatcaacg gcgggttaaa gggattgatc
    50101 cgcgtgatat gcaaccgctg cttaatcctt ttccaccgat gatggacacg ttgcaaaata
    50161 tggcaacgcg tcccgcgctg tggatactgt tagtcgcgat tatcctgatg ctggtctggc
    50221 tggttcgtta gccccgacga aatgacagta tcgtgacaat aattcccagc accgcagaga
    50281 tcgccccggc aagaaatacc gaagagtaac caaacgtggt cgccagcatt cccgccagcg
    50341 gcccggagac gccgagggcg atatcctgaa acgcggcgta accgcccagt gcggtgccgc
    50401 gaacttgtga ggggacgcgt ttaaccacct ccacgcccag cgcaggaaag ataagcgaac
    50461 atccggctcc ggttaacgcc gcgcccgcta atgcgaccca cgcacctggg gcttgccaga
    50521 gcagcaacaa gcccaccgtt tctacaagca gagagacaat cgccactttc acgccgccaa
    50581 aacggtccgg catccagcca aacatgacgc gcatcacgac aaatgcgccg ccaaactcgg
    50641 taagagtaaa gcccgccatc gcccatcctt tgctggcaaa gtagagcgaa acgaaagtcc
    50701 cgataaccgc aaaaccaacg ccttgtagtg ccagccctaa ccctggtttc cagataaacc
    50761 cgacaacgct ccacagcgat ggacgttctc ccgccagggc cggtactttg cgcactgtgc
    50821 cgttacaggc ccacgccagt aagggtaata ccattgtggt gatcgccagt gcggcaaaac
    50881 cgtaatggct atgaatcaac aggccaagcg gagcaccaac agcgagggca ccgtaaatcg
    50941 ccattccatt ccatgacatc actttgccag agtgttttgg ccctacgatg cctaaccccc
    51001 aggtcagagc gcctgtcagt aactggcttt caccaaaccc aagaatcaaa cgcccgatga
    51061 ccaacagggc aaatttgaac ggtgcggaga caggcaaaat cgccgccagc agcaacgcgc
    51121 cgccagccag accacaagct aacatcccct gaagcgccga acgttttgcg ccatattgat
    51181 cggccagtcg cccggcatag ccacgcgtca gcaccgtagc cagaaactga atcccgacgg
    51241 caatgccgac catggtattg ccatagccca gttcatgatg aacaaacagc gggataaccg
    51301 gcaacggcag ccctacggtc atgtaggtga gaaaaaccgc aaaagcgatg cggaagagcg
    51361 aaaaattggc agaagatcgt gtttcggttt ggcttacagc agtcatgcat tactccagaa
    51421 tgcagcgcaa ggcgaggagt atccccgtct catctctctg gtttcagggt tacggtgcgt
    51481 tggcaggatt taacgcgtac gtcttttcag aaggaaatcg acaaagcggg aagtttgcct
    51541 ggaactggtg gcgattgtca atgatgtgaa aaagggaacc atcaggttcc cttttgcgtt
    51601 agtgccggag gccgcattgg cgcgtaacgt cggatgcgac gctggcgcgt cttatccgac
    51661 ctactccggc ctactgcgaa ttaatctttc agcttgcgca ttaccagcgt ggcgttggtg
    51721 ccgccgaagc cgaagctgtt agacataaca gtggtcagtt cgcgatcggt cgtttcggtc
    51781 acgatgttca gacccgcagc ctgctcgtcc agctcttcaa tgttgatgct cggggcgata
    51841 aagccgtgtt ccagcatcag cagagagtag atagcttcct gcacacccgc agcgcccagt
    51901 gagtgaccgg tcatggcttt ggttgcagaa atcgccgggc tcttatcgcc gaacacttca
    51961 cggatagctg ccagctcttt cacgtcgcca accggagtcg aagtaccgtg agagttcagg
    52021 taatcgattg gggtatcaac gccatgcatc gccatctgca tacaacgtac tgcgccttcg
    52081 ccagacggag caaccatgtc tgcaccatca gaggttgcgc cgtagccaac gatttcagca
    52141 tagatgtgag caccacgcgc cagcgcgtgt tccagctctt caaccactac cataccgccg
    52201 ccgccagcga taacgaaacc gtcacggtga gcgtcgtaag tacgggaggc tttttccggg
    52261 gtgtcgttgt atttagtaga cagcgcaccc atcgcgtcga attcgcaggc catttcccag
    52321 cacagctctt cgccgccgcc agcaaacacg atgtcctgtt tgcccagttg gatctgctct
    52381 actgcgttac cgatacagtg tgcggaagtc gcacacgcgg agctgatgga gtagttaacg
    52441 ccatgaattt taaacggcgt ggcgaggcag gcagaaacgc cggatgccat cgctttggtg
    52501 accacatacg ggccaaccgc tttcaggccg cgcgggccgc gcattgcgtc agcgccgaat
    52561 acctggaaac gcggggaacc gccgccagaa cctgcaatca ggccaacgcg cgggttattc
    52621 tggtaagctt ccggagaaag gcccgcatct gcgattgcct gctccataga aaggaatgca
    52681 taaatggatg cgtcgctcat aaagcgcaca actttgcggt caatgaggcc agtggtatcc
    52741 agttttacgt tgccccagac gtggctacgc atgccggaat ccttcagctc ctgagagaaa
    52801 gtgatccctg aacgtccttc acgcagagat gccaggactt cctgctggtt attaccgatg
    52861 ctggaaacaa tgcccaggcc agtaatcact gcacgtttca ttcaatacct ctgtaagtcg
    52921 cacatagagt aagtttcgaa tgcacaatag cgtacacttg tacgccgaac aagtccgatc
    52981 agccatttaa tagagaaatt tgcgcagcct tacacacatc gctaagatcg agccaccgcc
    53041 tgtaagacga gtaacttacg tgaaacacta ctccatacaa cctgccaacc tcgaatttaa
    53101 tgctgagggt acacctgttt cccgagattt tgacgatgtc tatttttcca acgataacgg
    53161 gctggaagag acgcgttatg tttttctggg aggcaaccaa ttagaggtac gctttcctga
    53221 gcatccacat cctctgtttg tggtagcaga gagcggcttc ggcaccggat taaacttcct
    53281 gacgctatgg caggcatttg atcagtttcg cgaagcgcat ccgcaagcgc aattacaacg
    53341 cttacatttc attagttttg agaaatttcc cctcacccgt gcggatttag ccttagcgca
    53401 tcaacactgg ccggaactgg ctccgtgggc agaacaactt caggcgcagt ggccaatgcc
    53461 cttgcccggt tgccatcgtt tattgctcaa tgaaggccgc gtgacgctgg atttatggtt
    53521 tggcgatatt aacgaactga ccagccaact ggacgattcg ctaaatcaaa aagtagatgc
    53581 ctggtttctg gacggctttg cgccagctaa aaacccggat atgtggacgc aaaatctgtt
    53641 taacgccatg gcaaggttgg cgcgtccagg cggcacgctg gcgacattta cgtctgccgg
    53701 ttttgtccgc cgcggtttgc aggaagccgg attcacgatg caaaaacgta agggctttgg
    53761 gcgcaaacgg gaaatgcttt gtggggtgat ggaacagaca ttaccactcc cctgctccac
    53821 gccgtggttt aatcgtacgg gcagcagcaa acgggaagta gcgattatcg gcggtggtat
    53881 tgccagcgcg ttgttgtcgc tggcgttatt acggcgcggc tggcaggtag cgctttattg
    53941 cgcggatgaa gcccccgcgc tgggtgcttc cggcaatcgc cagggggcgc tgtatccgtt
    54001 attaagcaaa cacgatgagg cgctaaaccg ctttttctct aatgcgttta cttttgctcg
    54061 tcggttttac gaccaattac ccgttaaatt tgatcatgac tggtgcggcg tcacgcagtt
    54121 aggctgggat gagaaaagcc agcataaaat cgcacagatg ttgtcaatgg atttacccgc
    54181 agaactggct gtagccgttg aggcaaatgc ggttgaacaa attacgggcg ttgcgacaaa
    54241 ttgcagcggc attacttatc cgcaaggtgg ttggctgtgc ccagcagaac tgacccgtaa
    54301 tgtgctggaa ctggcgcaac agcagggttt gcagatttat tatcaatatc agttacagaa
    54361 tttatcccgt aaggatgact gttggttgtt gaattttgca ggagatcagc aagcaacaca
    54421 cagcgtagtg gtactggcga acgggcatca aatcagccgt ttcagccaaa cgtcgactct
    54481 cccggtgtat tcggttgccg ggcaggtcag ccatattccg acaacgccgg aactggcaga
    54541 gctgaagcag gtgctgtgct atgacggtta tctcacgcca caaaatccgg cgaatcaaca
    54601 tcattgtatt ggtgccagtt atcatcgcgg cagcgaagat acggcgtaca gtgaggacga
    54661 tcagcagcag aatcgccagc ggttgattga ttgtttcccg caggcacagt gggcaaaaga
    54721 ggttgatgtc agtgataaag aggcgcgctg cggtgtgcgt tgtgccaccc gcgatcatct
    54781 gccaatggta ggcaatgttc ccgattatga ggcaacactc gtggaatatg cgtcgttggc
    54841 ggagcagaaa gatgaggcgg taagcgcgcc ggtttttgac gatctcttta tgtttgcggc
    54901 tttaggttct cgcggtttgt gttctgcccc gctgtgtgcc gagattctgg cggcgcagat
    54961 gagcgacgaa ccgattccga tggatgccag tacgctgacg gcgttaaacc cgaatcggtt
    55021 atgggtgcgg aaattgttga agggtaaagc ggttaaggcg gggtaatctg ctctcgcagt
    55081 gtttgccgga tgcggcgtga acgccttatc cgacctacgt gtgacgtgta ggcctgataa
    55141 gacgcgataa gcgtcgcatc aggcatggtg ctccagacgc cgcaacttac tgctgtgacg
    55201 cctgctgaaa caggttttcc cacatatcgg tcaccagcgc ctggtcacgc ggcgacaatt
    55261 caccggcacc aatggctttt tccagactct ggctaacggt cgtatgcacc gcctgagcgg
    55321 agtggtcatc accactttcc agttctgcga tggctaatgt caggtggcca cgcaaatacc
    55381 cactggcaaa caactcatca tcactggcat ggtcaaccat gccgtcgatt aatgccagaa
    55441 tgcgtgattc aaactccgcg atcatcttct ttcctcattg taaaacgtgg caccggcttc
    55501 agttgagcgc ttccggccac gggaactgtt ccgccgtaag ttccggcgtg tgataataat
    55561 tttgtagtgc tttaatgaag cgtgccggac gctcgggaat gccatttgtc agataatcca
    55621 tcacctgcgc atgtacccgc cgttgaaaca caacacgatc cggttcgaag tcgccttcca
    55681 gattgtcgca actgacgtta aacggatatc ccgccgccac acagaacaac cagtcgagtg
    55741 cctgcggctt cacttcaaca tcttcaaact ggctttgcgt ttgggcatca cgcccatccg
    55801 ggcaatacca gtagccgaaa tcaaccagct cacggcgcgc ttttccggca atacaccagt
    55861 gcgaaatctc atgaattgcg ctggcataaa agccgtgggc aaagacaatt cggttatacg
    55921 gtacttccgc atcagcagga agatagatcg gttcgtcgtc gcctttaatc agacgggtat
    55981 taaattcatc ggcaaagcag ctattaaaaa tttcaatcaa ctgctcatag tggtgtgtac
    56041 tgttcattag ttcatcccca accagtggag gatctcctgt ccgtggctat cgtaaagtaa
    56101 tttggcactc atcaccgccg agacgataac aatcatcggg cggatcagct tttgtccttt
    56161 gctcaacacc agccgtgaac ccatgcgcgc gccgaggaac tgccccacca gcatgacaaa
    56221 gcccgtcgcc caaatcactt tgccgccgag aataaacagc agcagaccgc caatgtttga
    56281 cgttgcgttg agtaatttgg cgtgagccgt ggctttggcg aggttgaatc cgcacagcgt
    56341 aacgaaggcc agcgcgtaaa acgatccggc agccgggcca aagaatccgt cataaaaacc
    56401 gacgcagcca ccggcaatta acgcgaaggg taaaccgtac atccggcgct ggcgatcttc
    56461 ttcacccagc tttggcatca gcaagaaata gaggccgata caaatcacca gaatgggcaa
    56521 aatctgccgt aagacatcag cctgaacgta ctgtaccaac agtgcgccgc tcattgagcc
    56581 gacaaaggtc atggcaatat tgagtttctg atcgcttaaa ctaaccactt tgcggcgaat
    56641 aaagtagata gtggcggaaa tagagccgcc gcaggcttgc agtttattgg ttgccagcgc
    56701 gttagcggga gacattcccg cagccatcaa tgccggaatg gtgagtaacc caccgccacc
    56761 ggcaatcgag tcgataaatc ccgccagcat ggcgacaaaa aagagaactc ccagcaacag
    56821 cggggaaacc ataaacaggc tattaaacgt ttccattaga tcacgtgctc atccagtagc
    56881 gcctggcagg aaggcggcaa cggaggcggt gtcttcttct caggctttgt tgttcccggt
    56941 tttagaggtg caaaccagct ttgcagttct gccccgcaac catcgcctga tggcggtaaa
    57001 ggttgatctt cacactccag actatcggca gggcaacgta atcgtacatg catatgcgcg
    57061 cgatgctgga accagggtcg cactttgcgc aaccagtcgc gatcggtgcc cgcatcaagg
    57121 caaagttgtt gtttaatcgc cggattaaca aaaatgcgcg tgacgtcttt gtcctgggca
    57181 gcgagtttga tcaagctgaa aatttctggc ttccacagcg tggagacaac gtgtttaccg
    57241 tcgcgggaaa ctaagtccag tgcttgcggg cgcaagagct gcgcggaggt ccagcgagtt
    57301 ttcggcagtt gcagaaagat atcgacatcc agtccggtct ggtggctggc atgaccgccg
    57361 ttgaaacgcc caccagcggg catccccata tcgccaatca gcaccgtacc catgcccaga
    57421 ttgctcacct ggctactcag acgctggata aacatcacca gatccgggtg accgaaatag
    57481 cgacgctgat cggtacgcat gacctgataa tgttcggact gtatcggcaa cgtgtcagcg
    57541 ccgacaatac agccattaga aaaactgcct atcgattgtg cgctacccgg cacaggttgg
    57601 gttatttttt gccacggcgt cgctgccagg ctagcgctac tggcaagcag agccagcagc
    57661 gcaatcgcgg ttttgttcat tttttaccag cgtggaatat cagtcttcac atcggcattt
    57721 tgcgcccgct gccgtaacag gtgatccatt aaaacgatcg ccagcatcgc ttcagcgatc
    57781 ggcactgcac ggatcccgac acagggatcg tgacggcctt tggtgatcat ctcaacttct
    57841 tcgccaaagc ggttaatagt gcgccccggc acggtaatgc tggaggtcgg tttcagcgcc
    57901 atatgagcaa tgatttgctg cccgctgctg ataccgccga gaatgccgcc cgcatggttg
    57961 ctctggaaac cgtctttggt gatttcgtcg cggttctggc tgccgcgcag cgccactacg
    58021 tcaaaaccgt cgccaatttc cacgcctttc accgcgttga tgctcatcag cgcatgggcg
    58081 atgtcggcat ccaggcggtc aaagaccggc tcgccaagtc cggcggggac accactggca
    58141 acaacggtga ctttcgcgcc gatggagtcg ccctcttttt tcagcgcgcg catcagttca
    58201 tctaacgcgt cgattttgtc cgggtccggg cagaagaacg gattttgttc aaccagcgac
    58261 cagtctttga tttccagcgg aatgtcgccc atctgggtca agcagccgcg gatttcaata
    58321 ccaaatttct cggcgagata ttttttggca atcgcccctg ccgccacgcg catggcggtt
    58381 tcgcgggcgg aagaacgtcc accgccgcga taatcgcgca gaccgtattt ttgttcgtag
    58441 gtgtaatcag catggcctgg gcggaaaacg tctttaattg cgctgtaatc ctgagagcgc
    58501 tggtcagtgt tttcgatcaa caagccaatg ctggtgccag tagtaacgcc ttcaaaaaca
    58561 ccggagagaa ttttgacctg atccggctcg cggcgctggg tggtatagcg cgatgtccca
    58621 gggcgacgac ggtcgaggtc atgttgcagg tccgcttccg tcagcggaat gcctggcgga
    58681 acaccatcga cgatgcagcc gagcgccagc ccatgcgatt cgccgaaggt ggttacgcga
    58741 aagagttgtc caattgtgtt tccagccatc acggctccgt tattgttgtg tttgcgtgtt
    58801 tacttaatct ttataaatcg cgaaatattc acgggccgcc agcagctgct ctttggtgag
    58861 cataaacaca ccatcgccgc cgttatcaaa ctccagccag gtgaacggaa catccggata
    58921 ttgttccata agatgtacca tgctgttgcc gacttcacaa atcaacacgc catcatcagc
    58981 aaggtaatct gccgcgtttc caagaatgcg acgcgtcagt ttaaggccgt cggtgccaga
    59041 tgccaggccc agttccggct cgtgacggta ttcgtttggc aggtcggaca tatcttccgc
    59101 gtcgacatac ggcgggttag tgacaatcag atcgtactgc actttcggca aatcacggaa
    59161 cagatcggaa cgaatcggaa tgacgttgtg gatcagaccg tgttcttcga tgttctgttc
    59221 ggcaaccgcc agcgcgtcgg gagagatatc taccgcgtcg acttccgctt ccgggaaggc
    59281 ataagcacag gcaatggcga tgcagccgct accggtacac atatctaaaa tatgctgcgg
    59341 ttgcttgcta atgagtccgg caaatttatt gttgatcagt tcaccaatcg gcgagcgcgg
    59401 caccagcacg cgttcatcga cgtaaaattc atgaccgcag aaccaggctt tgttggtcag
    59461 gtaagccacc ggaatgcgct cgttgacgcg gcggatcacg cgttcaacaa tacggtgttt
    59521 ttcgctggag gtcagacgcg cggtgcgcat atcttccggg atatccagcg gcaggtagag
    59581 cgatggtaac accagctgta cggcttcatc ccacgggtta tcggtgccgt gaccgtacca
    59641 gatatttgcc gcgctgaagc ggcttaccga ccagcgcaac atgtcctgaa tggtttgcag
    59701 ctcatttact gcttcatcaa cgaaaatttt atccacgtat tcctccaggg catgatcgca
    59761 ataatttcgg cggctagttt gccatgaaga tgacgataaa tcagcattca cgcgcggtga
    59821 gtgaggaaaa atacgtttaa aacgatcgat tgtgctacga gtcgggtaaa ctgtaggaaa
    59881 attagaaata gagacagata aatgaaaaag aaaacaacac ttagcgagga ggaccaggct
    59941 ctgtttcgcc agttgatggc ggggactcgc aagattaagc aggacacgat tgtccaccga
    60001 ccgcagcgta aaaaaatcag cgaagtgccg gtgaaacgct tgatccagga gcaggctgat
    60061 gccagccatt atttctccga tgagtttcag ccgttattaa ataccgaagg tccggtgaaa
    60121 tatgttcgcc cggatgtcag ccattttgag gcgaagaaac tgcgccgcgg cgattattca
    60181 ccggaattgt ttttggattt acacggtctg acgcagctgc aggccaagca ggaactgggc
    60241 gcgttgattg ccgcctgtcg ccgcgaacat gtgttttgtg cctgcgtgat gcatgggcac
    60301 gggaagcata ttttgaagca acaaacaccg ctgtggctgg cacaacatcc gcatgtgatg
    60361 gcatttcacc aggcaccgaa agagtacggc ggtgatgcgg ctttgttggt gttgattgaa
    60421 gtggaagagt ggttgccgcc ggagttgccc tgaatgctgg cagcaaatgc cggagcagcg
    60481 taaacgcctt atccggccta caaatatgtg ataattcaat aaattgcaat tcacttcgta
    60541 ggcctgataa gacgcatcag agcaatacta cgaactacag ttcattcaat acatactgaa
    60601 aacgacacgt cgaatcaatc ggttgcccac tactggtatt cacattcacc acaatatcgt
    60661 gtagtacttt atccgttacc ggatgacgag ttggcgtaac cgttaccgtt gatttatcac
    60721 aaccgtaaga ggtaaaggtt aacaattctg ataccgcgcg gcagtatgct cctctgccat
    60781 cattatcaga aaaattactg ccatcacaga gtctgccgcc ttccatcagg taggtcgaaa
    60841 gcgtggtggt cactgttgac cccgtggact gaattcgtaa cgccttatta tggctgacat
    60901 aattttcact ccaggagata tcgtatgaag tgaccggtat cgttgggcat attgtgttat
    60961 gcactgtagg ggccgtatag cttccaaatc cgggtaaatc tgtataggaa taaaaggatg
    61021 cgatccaagg gcaaccataa tcatcagatg caacggtaat tttaaacgtc atatatgcac
    61081 aacgaaaaat tttcgtatct atgccagggc aatttgagtt attaattgta tcaaagtcag
    61141 tcagtaccaa agttggagct gaacccgacc cagaccagga aataacccgc aacgtatcgc
    61201 ctttatacgc aaaagaatca tatttcccat atgtaacctc ctttggcgtt atcaccgtaa
    61261 cattgaaaac actgctcgct acagcaccgg ttaccttgtc catggcataa taaactctca
    61321 cactgtcaaa actcgcactc ataagtttat ctccagcata actcttagat gcgccgacaa
    61381 cactcagaac gagcagcatt aggctcgcga aaattatggt ccattttttc atttcagaaa
    61441 tactcaaagg ttgccgtcac tgtggcacta aagtctccgg acgttacatc tcgcccgctc
    61501 tttgcctgta accaggtatt aaaatttagg ctgttatttc cctgattcag cgggagcgtg
    61561 acgcctgttg tattgttgat tggcaccgat gtgcctgccg cagtttcaat accaatccca
    61621 gcgccggatg ctgatgacga ggagtcaaag gccagaaagc cgggtagagc actatcttct
    61681 gttcctgtca acgttacttt gacattaaac aatgtagaac tatgacaatc tttcaattga
    61741 aacaccaatg gcactcgctc tgactgccca aggttcatta aatcgctggc ggcaatggca
    61801 ggaaaatgta cttctgccaa ctggctacca ttaatcactg gcgtacacga tttactgatt
    61861 aaattgccgg taaagtgcaa attatctcct gctattgtgg ttccactgcc cagcaacatc
    61921 aggctactaa taaaaaacaa cgtacgcttt ttcttcattg gtaatctacc tgtaacgtgg
    61981 cccaggcctc aaagtccgcc tctggcagaa cgacaccact cttttttact ggcacagccg
    62041 ttaacaccgg taaatcggtt tcattcacca taagtggcgt attgatcgta aaactctgcc
    62101 ctgcctgttt gagctgaatg cctaaaccag tgatattgct gcttactgca gcattatcaa
    62161 aatcgcttag tgtgccactc cagctcaagg tcatttccat taatgggtcg cgaaccgccg
    62221 tatcgcaggt aatggaatac ggcacggttt gctcataatt gctgccatta atgtcatcaa
    62281 taagcacatc ctgaaaagtt atttcaatgg tttgagcgtt gctaatcgtg cagttaggcg
    62341 gctgcactaa ataaccgtga aaagagacgt ttgagagtgc catcgagcaa aagccccaca
    62401 gcaaaataag cgagatccgt ttcattggta atccaccaca attgtggcac tggcgttaaa
    62461 ttcagctgcc gctaacgttg taccactctg tttcaccggc acagcttcca gaacgggaac
    62521 tcccgaggaa aaattaaatg gcaaccatga gccagaggtt aagtcgagta ttgtgtgatc
    62581 actacttttc tgaacccgaa taccaaaccc aggaataccc gtgctgataa ctgtttcacc
    62641 gttaatcacg accgttgtcg cctgcatctg tagacgcagg tcatccattg ccagattgtt
    62701 acaaacaagc gagtagtcaa tgggacgttt ataatcaacc ccattgatct tattaatgaa
    62761 tacattacca aactcaactg ccgcaccatt taccgtacaa ggaggagggg catcgacaag
    62821 cacccgtaaa ttcatactta ctgacttact cgctgcaact gcaaagccgc taccaacaca
    62881 acatatcagt aacccagtta ccgcccagac agaacaagat cctttcatac tggctctccc
    62941 ggcctcagcc tttgcggctc tgctcttcat cgacctggca actgttaccg ttgcaagtga
    63001 aaaagagcgg catccgagcg ccatagtcgt tgacataggt cagtaccggc acgctaccca
    63061 tcttcacatt cagcggtgca gaggttttcg gcgacatcac tattggcgag aaaccggctg
    63121 caggattgcc atttttctgt gtgctggcat tactgaaaat gacgtaatac ggcgtcgggt
    63181 tattgacggt aaattcctga ccgttgcggg ttaaagtaac cttatattgc caggggtttt
    63241 tcatgctgac gttctccagc gccttcggtc gccagaacaa cttaatacgc gtttgtagtg
    63301 ctatttgcag cgtgttggct ttgtttgatt ttggcgggat ctcacgcacg ttgaagtaga
    63361 acaggctttc acgatcggca ggcaatttgt tgatatccgg cattccctgc acttttacct
    63421 gcccgttcat cattgaatcg atgcgttgca ccggtggcag aacagttaac ggtgaactga
    63481 tcttgttacc cttttcatct tcgatccagc tttgggccag ataaggcagt ttcggatcgt
    63541 tattgcgcaa cgtcacgctg attgatttgt cgctttcatt aaatatcaaa cgggtacgat
    63601 ctggcgttac agaagcctgg gcgacaaact agcagcgcta agcagtaacg ctagcgtcgt
    63661 agcacccaat ttggcagagc aaagtaaatc agacatggat cattccttaa tcacgttatt
    63721 ggttagctga aacagaaaca gggggattgg tgggtgtcac ctgttgcgtc tgctcctgaa
    63781 tgacaggctt tatgtcatca ggaacaaccg gcgcgacgtt gcctttatgc tggcatggca
    63841 gtaacaagcc gttaaacaga tcggcaggca gcgggtccgg caggttgata tcgcaatgcg
    63901 caacaccact ccagaacaca ctcatgtgtt cgccaggttt caccccagcc agataaacat
    63961 tgccgtcatc atcgacaagg cccactgtct gctcgttatc attttttact tctgcgccaa
    64021 acggaggatg gctgccgtct tgtaaacgca gcaccgccat cgctttttga ccactgatga
    64081 cggcaaattt gcggtagcca atcgcccctt cagttagcgt ggcttgtacc accgactggg
    64141 ttgcttcggc gttttccggc agtttgttga ggtcgatata cgcctgattg cggtaatagt
    64201 tattgacgtc agaaacgacg gctttaccaa acatattggt ataaacagca gcaccgttac
    64261 cttctaccgg aacatcggca acgccatcgg catcaatcaa caagcgtgtc ccgcccatat
    64321 tctgggtacg gtgaagtgcg ccaccgtggg cagtaagcgt tgcgccgccc tgtaacgaca
    64381 agcccgcaga ggtgtattgc ccttcatggt agttcgcact gaggtcaacc tgcgccagcg
    64441 aaccatcatg gctgtaatag ccgtcaacgc tggtgtgttt gtcactggtg ccgacgttca
    64501 actgatagtg agtcgcgtca tcgacacggc tgaaataacc gacctgactg ctgtccgtcc
    64561 cactgccata gttaccgtta tagctaacgg tactgttgtc accccacggc atactgagcg
    64621 aaatgtacat gcctttgtcg gcctggttgt catactcgta acggtagcca gtcatcgaga
    64681 tgctgacatt acgaatactg cccatattga aatagtgcga gagcatgatg ttgtagtttg
    64741 tctgctcctc gcgatcccag taggtatggc gggtatagtt gagataaacc gaaacacccg
    64801 catcgcggaa gttctggtta taagtggcag tgtacatctc tttgtcgttg cccgtgcgta
    64861 ccattccgct gtcactggca tccagatact cgctcatggt cataaagttc tcttccgaga
    64921 agcgatatcc agcaaaagta acgcggctgt tgagctggtc aaagtctttg gaataactca
    64981 cacggaagga gttaccgtcc agcgaacctt tgccataagc ggtgtcttta tccagtttgg
    65041 tgtgcgagtg agtaacatca aacgcgaccg cgccgaatgt agacaaatcg cgaccgacgc
    65101 caagcgccgc agactgatag ttttcatctc ccagtgcgcc gccatataac gaccagccgt
    65161 tagcgatccc ccaggaagct tcagcaccag aaaaaaattc accctcgaca tggtgtcccc
    65221 actcttgcgg acggcccatc atgatcttat agcgaacctg acctggacga gtgaggtatg
    65281 gcatcgaggc ggtgctgatg tcatattcct gcacctggcc gttctgttct tcaatgcgaa
    65341 tatgcaacgt accggagaca gaatcaccaa gatcctgaat acgaaacggt ccagccggaa
    65401 cctgcgtttc gtaaatcaca cgccccatct ggctgacggt cacttttgct gtggtgtgtg
    65461 ccacgccgga aatgtctggc gcgtagccgc gcagattggg aggcaacatt tgatcgtcag
    65521 tactgacact gccaccaaca tagttaaaac catcaaaaat atcggatctg aggtaatcct
    65581 cgcccagcgc cagtttggct tttaatgatg gtaacgcccg ctaggcatag tagcgactcc
    65641 actcccattt tttttgagtt tcatcgccgc tgaattcatc gtcatcatta ctgcgagtat
    65701 gttgatagtt agtctgccag tcagcacgca tacgccacgg ccccaggtta accccgaccg
    65761 tcccgttgcc gctgatctcg ttactatcat caccgccatt ttcttcgtgc cgggtttgtg
    65821 cgttgatgct gtagtccgcg acgatcccgg agatgccgtc atcccaacgt gaaggcggat
    65881 cccagtcggg ataagtatat tcgaggtaag cctgtggcag tgaaatgact aatgcggact
    65941 ggcttaaatc agccttaatt tccatgcctt ccagttgacc ggatttcagg catttagcat
    66001 cgtggctcca ttgcagattt ttcgccacgt cttcttttaa accaaactgc gctaccagtt
    66061 ccggtgtcag acaagcatag cttttgctcg cgtcatcttc accagcatac cagtaaatat
    66121 cgtactcttc cgccaatggc tgtttattta gttgaacctg taaattgtat tttccgggct
    66181 caacatatcc ctggctggaa aaacgcttca gatcaatttt tgtgtcgcct tttaattcca
    66241 gaaaacggga atcaaactga atgtcgtctt cagcccagac actgctataa ctccctgaca
    66301 ttgccagcgc aatgcaccac ggaagaatcc gtaatcgaaa aagagaatgg tcaggcatac
    66361 acgtttctat ccgtttttat tcttgttcgg tcaaaatcca tttaaatccc ccatagcagt
    66421 gtcgctatgg gggtatgcct tacaggtaag taatctggaa ggtggtattt gcttcgaaat
    66481 tacccagatc tggcgcatca gctgcgccga ccagccaggc tttaaagttc agtgtctgct
    66541 tcgctttgcc tttattagta gaagtgtctt tcactatttt ctggtcaata cccatgccgc
    66601 ttttgtaaga ggtacccaga gagtcaccga tcgccaggct aacattgtta aatgccgcac
    66661 aggtatcggt attgaaaatg gtgtaataat tgccgctatt tgcagaagaa acggtaccag
    66721 taaaggtagt ggtcattgtt tcctgagtat caaatacgca atcttgcaga cgaatctgga
    66781 aacttttcgg cgtggtggtg ccgttatttt tcagtttttc ggcaccgata tcacccatat
    66841 caacttccag tttgtgatcg tccggaacga tagaacacac tgagtcttca atggtgccgt
    66901 aaaaacgtgc agtaccgttg ttacccgcag cgattgttgc cgtagaggtg aacacgccca
    66961 tcaccattgc cgcagcaata gctgttttaa caaacttact cattatttgt ccttaaacta
    67021 taaataatta aaaatataaa cattgcaata cattgattca gtcaataacc aatgtttata
    67081 cccagccatt gaatacggca tgcgatccaa aaagaacagg tcgtagaaaa ttgtattcgt
    67141 tggtgtgggg ccgcatagtg atgtttccac acc
//
LOCUS       DAZPLI010000004        64586 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZPLI010000004 DAZPLI010000000
VERSION     DAZPLI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64586
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.004"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(35..1333)
                     /gene="eno"
                     /locus_tag="ACHUU3_000220"
     CDS             complement(35..1333)
                     /gene="eno"
                     /locus_tag="ACHUU3_000220"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR2080402.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="ACHUU3_000221"
     CDS             complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="ACHUU3_000221"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR2080403.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="ACHUU3_000222"
     CDS             complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="ACHUU3_000222"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR2080404.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="ACHUU3_000223"
     CDS             complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="ACHUU3_000223"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFR2080405.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="ACHUU3_000224"
     CDS             complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="ACHUU3_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFR2080406.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4809..7043)
                     /gene="relA"
                     /locus_tag="ACHUU3_000225"
     CDS             complement(4809..7043)
                     /gene="relA"
                     /locus_tag="ACHUU3_000225"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR2080407.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="ACHUU3_000226"
     CDS             complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="ACHUU3_000226"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR2080408.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8449..11205
                     /gene="barA"
                     /locus_tag="ACHUU3_000227"
     CDS             8449..11205
                     /gene="barA"
                     /locus_tag="ACHUU3_000227"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR2080409.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11437..12777)
                     /gene="gudD"
                     /locus_tag="ACHUU3_000228"
     CDS             complement(11437..12777)
                     /gene="gudD"
                     /locus_tag="ACHUU3_000228"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR2080410.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12798..14138)
                     /locus_tag="ACHUU3_000229"
     CDS             complement(12798..14138)
                     /locus_tag="ACHUU3_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR2080411.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14140..15492)
                     /gene="gudP"
                     /locus_tag="ACHUU3_000230"
     CDS             complement(14140..15492)
                     /gene="gudP"
                     /locus_tag="ACHUU3_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR2080412.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15927..16376)
                     /locus_tag="ACHUU3_000231"
     CDS             complement(15927..16376)
                     /locus_tag="ACHUU3_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR2080413.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16394..17176)
                     /gene="truC"
                     /locus_tag="ACHUU3_000232"
     CDS             complement(16394..17176)
                     /gene="truC"
                     /locus_tag="ACHUU3_000232"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR2080414.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17176..17505)
                     /locus_tag="ACHUU3_000233"
     CDS             complement(17176..17505)
                     /locus_tag="ACHUU3_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR2080415.1"
                     /translation="MTTHDRVRFQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18127..18672)
                     /gene="syd"
                     /locus_tag="ACHUU3_000234"
     CDS             complement(18127..18672)
                     /gene="syd"
                     /locus_tag="ACHUU3_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR2080416.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18740..19588
                     /gene="queF"
                     /locus_tag="ACHUU3_000235"
     CDS             18740..19588
                     /gene="queF"
                     /locus_tag="ACHUU3_000235"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR2080417.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19700..21064
                     /gene="ppnN"
                     /locus_tag="ACHUU3_000236"
     CDS             19700..21064
                     /gene="ppnN"
                     /locus_tag="ACHUU3_000236"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR2080418.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21620..22909
                     /gene="sdaC"
                     /locus_tag="ACHUU3_000237"
     CDS             21620..22909
                     /gene="sdaC"
                     /locus_tag="ACHUU3_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR2080419.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22967..24334
                     /gene="sdaB"
                     /locus_tag="ACHUU3_000238"
     CDS             22967..24334
                     /gene="sdaB"
                     /locus_tag="ACHUU3_000238"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR2080420.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24446..25201
                     /gene="xni"
                     /locus_tag="ACHUU3_000239"
     CDS             24446..25201
                     /gene="xni"
                     /locus_tag="ACHUU3_000239"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR2080421.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25306..26454)
                     /gene="fucO"
                     /locus_tag="ACHUU3_000240"
     CDS             complement(25306..26454)
                     /gene="fucO"
                     /locus_tag="ACHUU3_000240"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR2080422.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26482..27129)
                     /gene="fucA"
                     /locus_tag="ACHUU3_000241"
     CDS             complement(26482..27129)
                     /gene="fucA"
                     /locus_tag="ACHUU3_000241"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR2080423.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27676..28992
                     /gene="fucP"
                     /locus_tag="ACHUU3_000242"
     CDS             27676..28992
                     /gene="fucP"
                     /locus_tag="ACHUU3_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR2080424.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            29025..30800
                     /gene="fucI"
                     /locus_tag="ACHUU3_000243"
     CDS             29025..30800
                     /gene="fucI"
                     /locus_tag="ACHUU3_000243"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFR2080425.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGSVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            30909..32327
                     /gene="fucK"
                     /locus_tag="ACHUU3_000244"
     CDS             30909..32327
                     /gene="fucK"
                     /locus_tag="ACHUU3_000244"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFR2080426.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            32329..32751
                     /gene="fucU"
                     /locus_tag="ACHUU3_000245"
     CDS             32329..32751
                     /gene="fucU"
                     /locus_tag="ACHUU3_000245"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR2080427.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            32809..33540
                     /gene="fucR"
                     /locus_tag="ACHUU3_000246"
     CDS             32809..33540
                     /gene="fucR"
                     /locus_tag="ACHUU3_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR2080428.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(33584..34684)
                     /gene="rlmM"
                     /locus_tag="ACHUU3_000247"
     CDS             complement(33584..34684)
                     /gene="rlmM"
                     /locus_tag="ACHUU3_000247"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR2080429.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34677..35072)
                     /locus_tag="ACHUU3_000248"
     CDS             complement(34677..35072)
                     /locus_tag="ACHUU3_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR2080430.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKCKGVSHE"
     gene            complement(35091..36008)
                     /gene="gcvA"
                     /locus_tag="ACHUU3_000249"
     CDS             complement(35091..36008)
                     /gene="gcvA"
                     /locus_tag="ACHUU3_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR2080431.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(36359..36586)
                     /locus_tag="ACHUU3_000250"
     CDS             complement(36359..36586)
                     /locus_tag="ACHUU3_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR2080432.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            36778..37983
                     /gene="csdA"
                     /locus_tag="ACHUU3_000251"
     CDS             36778..37983
                     /gene="csdA"
                     /locus_tag="ACHUU3_000251"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR2080433.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            37983..38426
                     /gene="csdE"
                     /locus_tag="ACHUU3_000252"
     CDS             37983..38426
                     /gene="csdE"
                     /locus_tag="ACHUU3_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR2080434.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(38477..39283)
                     /gene="tcdA"
                     /locus_tag="ACHUU3_000253"
     CDS             complement(38477..39283)
                     /gene="tcdA"
                     /locus_tag="ACHUU3_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR2080435.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(39360..40457)
                     /gene="mltA"
                     /locus_tag="ACHUU3_000254"
     CDS             complement(39360..40457)
                     /gene="mltA"
                     /locus_tag="ACHUU3_000254"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR2080436.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            40666..40742
                     /locus_tag="ACHUU3_000255"
     tRNA            40666..40742
                     /locus_tag="ACHUU3_000255"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40700..40702,aa:Met,seq:cat)
     gene            complement(40817..42070)
                     /gene="amiC"
                     /locus_tag="ACHUU3_000256"
     CDS             complement(40817..42070)
                     /gene="amiC"
                     /locus_tag="ACHUU3_000256"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR2080437.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            42302..43633
                     /gene="argA"
                     /locus_tag="ACHUU3_000257"
     CDS             42302..43633
                     /gene="argA"
                     /locus_tag="ACHUU3_000257"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR2080438.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(43695..45521)
                     /gene="recD"
                     /locus_tag="ACHUU3_000258"
     CDS             complement(43695..45521)
                     /gene="recD"
                     /locus_tag="ACHUU3_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR2080439.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(45521..49063)
                     /gene="recB"
                     /locus_tag="ACHUU3_000259"
     CDS             complement(45521..49063)
                     /gene="recB"
                     /locus_tag="ACHUU3_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR2080440.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(49056..51939)
                     /gene="ptrA"
                     /locus_tag="ACHUU3_000260"
                     /pseudo
     CDS             complement(49056..51939)
                     /gene="ptrA"
                     /locus_tag="ACHUU3_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
     gene            complement(52115..55483)
                     /gene="recC"
                     /locus_tag="ACHUU3_000261"
     CDS             complement(52115..55483)
                     /gene="recC"
                     /locus_tag="ACHUU3_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR2080441.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(55496..55819)
                     /locus_tag="ACHUU3_000262"
     CDS             complement(55496..55819)
                     /locus_tag="ACHUU3_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR2080442.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(55804..56211)
                     /locus_tag="ACHUU3_000263"
     CDS             complement(55804..56211)
                     /locus_tag="ACHUU3_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR2080443.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(56208..56771)
                     /locus_tag="ACHUU3_000264"
     CDS             complement(56208..56771)
                     /locus_tag="ACHUU3_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR2080444.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(56762..57232)
                     /locus_tag="ACHUU3_000265"
                     /pseudo
     CDS             complement(56762..57232)
                     /locus_tag="ACHUU3_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(57417..58211)
                     /gene="thyA"
                     /locus_tag="ACHUU3_000266"
     CDS             complement(57417..58211)
                     /gene="thyA"
                     /locus_tag="ACHUU3_000266"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR2080445.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(58218..59093)
                     /gene="lgt"
                     /locus_tag="ACHUU3_000267"
     CDS             complement(58218..59093)
                     /gene="lgt"
                     /locus_tag="ACHUU3_000267"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR2080446.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(59244..61490)
                     /gene="ptsP"
                     /locus_tag="ACHUU3_000268"
     CDS             complement(59244..61490)
                     /gene="ptsP"
                     /locus_tag="ACHUU3_000268"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR2080447.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(61503..62033)
                     /gene="rppH"
                     /locus_tag="ACHUU3_000269"
     CDS             complement(61503..62033)
                     /gene="rppH"
                     /locus_tag="ACHUU3_000269"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR2080448.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            complement(62386..62532)
                     /gene="ygdT"
                     /locus_tag="ACHUU3_000270"
     CDS             complement(62386..62532)
                     /gene="ygdT"
                     /locus_tag="ACHUU3_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR2080449.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            62718..63407
                     /gene="mutH"
                     /locus_tag="ACHUU3_000271"
     CDS             62718..63407
                     /gene="mutH"
                     /locus_tag="ACHUU3_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR2080450.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            63476..64189
                     /locus_tag="ACHUU3_000272"
     CDS             63476..64189
                     /locus_tag="ACHUU3_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR2080451.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            64327..64545
                     /gene="ygdR"
                     /locus_tag="ACHUU3_000273"
     CDS             64327..64545
                     /gene="ygdR"
                     /locus_tag="ACHUU3_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR2080452.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT        15655 a        17366 c        15822 g        15743 t
ORIGIN      
        1 gaggctggca tttttaaatc agataaagtc agtcttatgc ctggcctttg atctctttac
       61 gaccgttgta cggtgctttt tcgcccagag cttcttcgat acgaatcagc tggttgtatt
      121 tagcaacacg gtcagaacgg ctcatagaac cagttttgat ctggcctgca gcagtaccaa
      181 cagccaggtc agcgatggta gcgtcttcag tttcgccaga acggtgagag ataactgcag
      241 tgtagccagc atctttcgcc atcttgattg cagccagagt ttcggtcaga gaaccgatct
      301 ggttgaattt gatcaggatg gagttagcga tacctttttc gataccttct ttcaggatct
      361 tggtgttggt tacgaacagg tcgtcaccaa ccagctggat tttgtcaccc agaactttgg
      421 tctggtatgc gaaaccgtcc cagtcagatt cgtccagacc gtcttcgata gatacgatcg
      481 gatactgttt ggtcagttct tccaggaagt gagtgaattc ttcagaggtg aaagctttgt
      541 tgccttcgcc agccagaacg tatttaccgt ctttgtagaa ttcagaagct gcgcagtcca
      601 tcgccagagt gatgtctttg cccagttcat aaccagcagc tttaacagct tcagcgataa
      661 cagccagagc ttcagcgttg gaacccaggt tcggcgcata gccaccttcg tcaccaactg
      721 cagtgttcat gcctttcgct ttcagaactt ttgccaggtg atggaaaact tcagaaccca
      781 tgcggatggc ttctttcaca gttttcgcgc caaccggctg aatcatgaat tcctggatat
      841 caacgttgtt gtcagcgtgc tcaccaccgt tgatgatgtt catcatcgga accggcatgg
      901 agtatttgcc cggagtgccg ttcagttcag cgatgtgctc gtacagcggc atccctttag
      961 cggcagcagc agctttggcg ttagccagag atacagccag gattgcgttc gcgccgaatt
     1021 tggatttgtt ttcggtgccg tccaggtcga tcatgatctt gtcaatgcca gcctgatctt
     1081 tagcgtcttt gccaatcagt gcctgagcga tcgggccgtt taccgcagca acagctttgg
     1141 ttacgccttt acccaggaaa cgggatttgt cgccatcgcg cagttccaga gcttcacggg
     1201 aaccagtaga agcacctgac ggagcagctg ccataccgac gaaaccaccc tccagatgta
     1261 cttcggcttc aacagtcggg ttaccacggg agtcgatgat ttcacgaccg atgattttta
     1321 cgattttgga cattaggttt tcctcaagtc actagttaaa ctgaaactcc agacaaacaa
     1381 cgcgtaccca aggtacgcgt tgccgttcta acttttttac ttacttcgcc tgacgtttct
     1441 ggaactcgct ggcagctttc acaaagcctg caaacagcgg gtgaccatca cgtggagtag
     1501 aagtaaactc cggatggaac tggcaagcca cgaaccacgg gtgattcgga acttcgatga
     1561 tctcgaccaa ctgatcatcc ccggaacggc ccgcaacgcg cagacctgca tcttcaatct
     1621 gtttcaacag catgttgttg acttcgtaac ggtgacgatg acgctcaaca attgtcggcg
     1681 cattgtacag ctggcgaacc aggctatcgt caaccaactg gcactgctgt gcgccgagac
     1741 gcatggtacc gccgagatcg ctcttctcgc tacgaacttc aacgttgccg ttttcatcgc
     1801 gccactcggt aatcagcgcc acaaccgggt acttacagtc tggcacaaat tccgtagagt
     1861 tggcgttctc catgttggca acatggcgag cgtaatcaat taacgccacc tgcataccca
     1921 ggcaaatgcc cagataagga atattgttct cacgcgcaaa acgcgcggtc gtaatcatgc
     1981 cttctacgcc acgatagccg aaaccgccag gtacgaggat tgcgtccaga cctttaagga
     2041 tttcaacgcc gcgcgtttca acatcttgtg aatcgatcag tttgatgttg acgctgacac
     2101 gattcttcag cccaccgtgt ttcagtgctt cgatcactga tttataagca tccggcagtt
     2161 caatgtactt gccgaccata ccgatggtga cttcacttac cggattcgct tcttcgaaga
     2221 taacctgttc ccattcggac agatttgctt ccgggcagtt taagctgaat cgtttacaaa
     2281 tataatcgtc cagcccctga gatttcaaca ggcccggaat tttatagatg gaatcgacgt
     2341 ctttcagaga aataaccgct ttttccggaa cattacagaa caatgcaatc ttcgcacgtt
     2401 cgttcgccgg aacagcgcga tctgaacgac aaatcaggat gtcaggctgg ataccgatgg
     2461 agagcagctc ttttacagag tgctgagtcg gtttggtttt gacttcacca gacgctgcca
     2521 tgtacggcac cagcgtcagg tgcataaaca gagtgtgctc acggccaatt tcaacagcca
     2581 tctggcgaat cgcttcgagg aacggcaagg attcgatatc acctactgta ccgccgattt
     2641 ctaccagtac tacgtcatga ccttcgccac cttccagcac gcgctctttg attgcgttag
     2701 tgatgtgcgg aataacctgc acggttgcgc cgaggtagtc accgcggcgt tctttacgca
     2761 gaacgtcaga gtagatacga cccgtggtga agttgttgcg gcggctcatt ttggtacgaa
     2821 tgaaacgctc gtagtgcccc aggtccaggt cggtttcagc gccgtcttca gtaacgaaca
     2881 cttccccgtg ttggattggg ctcatagtac ctggatcgac gttgatgtac ggatccagtt
     2941 tcatgatggt cacattgagg ccacgggctt caagaatggc tgcgagggag gctgcggcaa
     3001 tgcctttacc cagagaggat acgaccccgc cggtcacaaa aatatagttc gttgtcatgc
     3061 tgaacctgag aagttaggtt gaaaagacga tggaataacc aggacgggaa agcagtatac
     3121 ccgaacatga cctgtgccac aaactttcat tatccctcct cttcgccagc gcactattga
     3181 aatcaggagt gagaaaatag cccctttggg gtaaatgttt ttgacgcaaa tcaagcgctt
     3241 gtcatttaaa aaatgacaca aatggcgctt gaccgcgtaa ttcccttaga gatcaatttc
     3301 ctgccgtttt acctgttgcc agacttcttc cattgtttcg aggtcaacac ctgtcatttc
     3361 cagtccacgc gcggcaacaa tacgctccac ttcgcgaaaa cgacgctcga atttttcgtt
     3421 cgctttttgc aatgcgattt ctgctttcgt ccctaaatgg cgagccagat taaccgtggc
     3481 aaacagcagg tcccccattt cctcctccag tttagcctgg tcgacaacag cctgccgcgc
     3541 ctcgtacatc acctcgtcga tctcttcgta gactttatca accaccgggc caagcgtcgt
     3601 ccagtcgaag ccaacgttgg cgcaacgttt ctggattttt tgcgcccgca ttaaagccgg
     3661 taaactacga ggaatatcgt ccagcgccga atgctgcgct ttctgcgcgc gctcttcggt
     3721 tttgatttgc tcccaacggg caagcacttc actactgttt tcggcagaac tatcagcaaa
     3781 aacatgcgga tggcgacgct ctaatttatc gctaatagca gcgcaaatat cattaaagtc
     3841 aaagcgccct tcttcctgag ccatttgcgc gtaaaacacc acctggaata gcagatcgcc
     3901 cagttcaccg cgaagatcgt caaaatcttc acgggcgatg gcgtccagca cttcgtaggt
     3961 ttcttcaagg gtgtaaggcg caatggtggc aaatgtctgc tctttatccc acgggcagcc
     4021 gttttccgga tcgcgcaggc gctgcataat agtgagcaaa cggtcgattt gattcattga
     4081 attgtcctga aaattgcggg tctgtcaggt ggaaacctgt gaccagaata gaagtgagtt
     4141 agtaacacta cccaatcagt acgttaattt tggctttaat gagttgtaat tcctctgggg
     4201 caactgttcc tttcttcgtt gctcctcttg cccgccaggc gatacttttt acctgatcag
     4261 ctaacgctac gccatcacgt tcctgaccgg ataaaacaac ttcgaacgga tatccttttg
     4321 attgcgttgt acaaggaaca cacagacaca tacctgtttt gttgttgtac atgaaaggac
     4381 tcaggacaac agctggacga tgtccagctt gctcgctacc ttttgtcggg tcaaaatcaa
     4441 cccaaatcag atcgcccata tcgggtacgt atcggcttac cattaccaga cttccttatc
     4501 tttcggctct ccccagtcga tattctcgtg gaggttttcc ggcgtgatgt cgttgaccag
     4561 ttcagcaagc gtaaatacgg gctctttacg cactggctca ataattaatt tgccatccac
     4621 caggtcaatc ttcacttcat catcaatatt cagattgagc gcctgcatta acgtagccgg
     4681 gatccgcacc gccggtgaat ttccccaacg ctttacgcta ctgtggatca taaccctttc
     4741 ctcaaaccgc tatcatatgt agatacagta tatatcaatc tacattgtag atacgagcaa
     4801 atttctgcct aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     4861 ttacccagca cgcggcccag cacctgcagg ttgtaaatct caatggtcat gtcgatggtt
     4921 gccagttgct gttttgtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     4981 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5041 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5101 gcatgagagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgc
     5161 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5221 tggtgcatca ggttgccaac accttcgact accacgcgac cgttatcttt actgcggttt
     5281 tgcggcgtgt agcttttttg ctgaagttgc ttcagcgcgg cggcgtcctg ctcttcggca
     5341 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5401 ccaccaatcg ccgccagcaa ctcgtcgaca tcattaaagt tgtaacgcgg cagcagatgt
     5461 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5521 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aagcgtgaat tttcgaacgc
     5581 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5641 tgtttctggg tgataatttc aatctggtcg cccatctgca gctggtaggt gaacggcaca
     5701 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5761 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     5821 acatacaccc ggtcgtcaaa gacctggctg cgtacttcgt cgagcatttc gccggaatca
     5881 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     5941 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6001 tctgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt gccacctggc
     6061 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacataatcg
     6121 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcatagcaa
     6181 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcaaaggcg
     6241 aggttctttt tctgcatttt acgccagatg ctgtagatgt gtttcggacg accatacact
     6301 tccgctttaa cgccttcagc tttcatctca gcgcgcagat gaccaacgaa ctcttcgatg
     6361 tagtgttcgc ggtcaagacg ccgttcatgc agcagtttgg caattcgttt gtattcggtt
     6421 ggatggaggt aacggaagca gtaatcttcc agttcccatt tcagttgtcc gattccgaga
     6481 cggttagcca gcggtgcgta gatgttggta cactcttttg ccgccagtac acgttcatct
     6541 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6601 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6661 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6721 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6781 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     6841 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     6901 ccctgcgtct gttgcagaca atacgcccaa gtttcggcta agcactcaca cgacttctgg
     6961 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7021 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7081 caaatcgcaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7141 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7201 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7261 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7321 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7381 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgccg
     7441 gaacgccttc tacacccacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7501 ccataccgca gaacaaatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7561 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7621 aagttaagcg caacccattt gagtcatacc agggcatctc accagagacg gtttcgagta
     7681 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7741 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7801 ttgcctgtac cagttcaaca tgaccaagat ggcgcataac ttgtaagctg cccaggcatg
     7861 ccatgacttt gggcagcaat gcttcaagtt ggggcactaa aatggggcat tgtttaacgt
     7921 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     7981 agtagttcag acttaaacgc gcgcgacggc gatagcccca gggaacatcg gcgatcactt
     8041 cagagacttc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8101 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8161 tttcgcgtcc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8221 tatcttcagt aacagtaact tccgcgtttt cctgcggcaa taatccgggg ataaatagcg
     8281 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8341 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8401 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8461 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8521 ctgagtatct ttttcgttgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8581 ggagccagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8641 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8701 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8761 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     8821 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     8881 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gctggagctg
     8941 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttccag cgtgatgatg
     9001 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9061 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9121 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9181 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9241 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttagatct ggcgaaaaag
     9301 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9361 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9421 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9481 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9541 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9601 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9661 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9721 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9781 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
     9841 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccggtctg
     9901 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
     9961 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10021 attatcgaag ggccatccac ccagtgcctc gccggtaagc gcctggccta tgtcgaacca
    10081 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10141 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10201 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10261 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagtcaatgc agaaaaactc
    10321 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10381 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagc
    10441 aagctggcaa tgacagtcat ggcagttgat gacaaccccg ctaacctgaa acttatcggc
    10501 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcggtt
    10561 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gcctgacatg
    10621 gatggcattc gggcctgcga gctcatccac cagctccccc atcagcaaca aacgccggtt
    10681 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10741 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10801 aagcctggca gcggtatttc ctctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    10861 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    10921 ctagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    10981 gaggagcagc tggttggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11041 ggcagttgcg gctatagcgg tgtgccgcgt atgaagaatc tctgccaact gatcgaacaa
    11101 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11161 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11221 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11281 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11341 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11401 gccggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11461 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11521 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    11581 acccagcccc ggtttttccg gcacctgaac cagaccgcct ttgatctcaa acggttcttt
    11641 ggtcaagcgc tgattgcctt cctgccaaat ccagtgcgta tcaatcgcgg tgattttacc
    11701 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11761 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    11821 catagtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    11881 gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg
    11941 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccat acggatcttc
    12001 tgcataagcc agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca
    12061 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12121 agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12181 atatttttca tacgccgctt ccgctaggcg caccaccgca tccggcgtca tcgcttcttc
    12241 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt
    12301 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12361 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12421 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12481 gcgcccacca gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    12541 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12601 ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat
    12661 attacgcgta aagaacggtg cgtgtgcccc actcagattc atcagcatac tgtcatgacc
    12721 cgccaccggg atgacctgca tttcagtaac aacagacgtc gtaaattgag aactcatatt
    12781 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    12841 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgccagg cagacgttta
    12901 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    12961 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13021 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13081 acatgggtaa acatcgccag agagatatcg aaatggttat tagaatggca gccccaggtc
    13141 agcccccagt cgtcgcaaag ctgcgccaca cggactgcac cggaaagcgt ccagaagtgc
    13201 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13261 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgccgaaa ctccgccata
    13321 acttcacgac cggagaaacc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    13381 tcattcagcc ctttgcatag agaaatggct tcatcaagta gccaggcacc gttgggatca
    13441 acggtaatcc gcgcatcagg gaagcgtttc ttcaatgcac gaacagtgtc gatttcttgc
    13501 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13561 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13621 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcaccg
    13681 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    13741 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13801 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccgaaggt atcaaaatcg
    13861 gctgcctgat tacctttatg cacctgctga accactttat tcaggcgcgc aacttcctgg
    13921 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    13981 tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca caatattgcg ggtgaaatat
    14041 gcgttatgtg ccccaccaat attaagcaac atgctatcgt gcccggcgac cggaatgact
    14101 ttcatatcag taataacagg actggattgt gtcgtcatct tattgccccg cgacaggttt
    14161 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattaaggc
    14221 atgaacacca acataaatca gcgccccatt aaaggagcca gtcgtgccaa cgatataacc
    14281 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14341 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14401 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14461 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14521 aatgttcagc gatcccgtgc gacgcatcag ccagtcggaa ataatcccac ccagcacacc
    14581 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14641 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    14701 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gttgtttgat
    14761 ctgcccccac ttcacgctga acggcacttt aactttggtg ttttgctgat ccatattgat
    14821 cagcgcgcca cccgcggcga tgtactccag ctctttctga tttacccccg gatgttgatt
    14881 tggctcgtgg atgactttca accagataaa gctgatgaca atccccagac cgcccataaa
    14941 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15001 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcgc
    15061 cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt cagcaagccc
    15121 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15181 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15241 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15301 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15361 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15421 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15481 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15541 acatagccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15601 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15661 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15721 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gttaaaacga
    15781 agaaataaag aagagtatga aatggatcgc ttgactccag gcaaacgcca gtaaaaatcc
    15841 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    15901 acgacattta ccgctcgctg aacatatcag gacaacagcg tgccccactg ttcgacccac
    15961 ggatttgatt ccgtttccgg ttccgggttc tcactggcgt caatcaacag catttcgcca
    16021 acccgctgag cgctctgttc ctgcaacaag gcatcgaact gtttgccgcc attgcagaaa
    16081 tttacataac tgctgtcgcc gagcgcaatc acgccataac gcagattcgg ctggaagccc
    16141 agactatctt tgattccctg aaagagcggc acaatgctat caggaaggtc gccctgcccg
    16201 gtcgtggacg taaccaccag aacatactta tcctgataag gcagccagtc gcttaattca
    16261 ggatcttcaa ataccgttgc tttgtggccc tgtgcggtca gaattgcttc tgcttcttcc
    16321 gccactaaca gcgaatttcc gtacatggtg ccgacaaaaa taccaatttc cgccataccg
    16381 ttttctccct ggattaggaa cttatctctc catcctgacc cgatggcgca ctgaactcaa
    16441 ccctttcatt ttcaggaagc agaccgcgcc agccaaattg tgataacgcc tgcatccagg
    16501 tgtcgtccag gcccgcgtga atagtcagcg gctcgccagt aaaaggatgc gtcaatgaca
    16561 actgactggc atgtagcatt aaccgctgga ggccaaaatg ctcagcaccg ctgcgattct
    16621 ggcgtaaatc gccatgtttg ctatcgccaa taatcggatg acgcaaatgg gcaagatgtc
    16681 ggcgaagctg atgtttgcgt ccggttttcg gctccagttc caccaggccg tagcgcgtgg
    16741 tcgggtaacg tccggtcgct accggcattt ctacggtcgc cagaccgcga taatgcgtca
    16801 ctgctggctg cgggccttta tcttcgcggg caaatttatc agcgattttg tccagttctt
    16861 ccaccagtgg ataatccagc accgcttctt ccatcaacca gccgcgcaca atcgcatggt
    16921 aacgtttctg gatttggtgc tgttcaaact gttgtgccag cagccgtccg gcctcgctgg
    16981 ataatcccat caacaacaca ccagaagtgg gtcggtccag acgatgagca gtaaaaacat
    17041 gctgtcctat ctggtcacgc acggtttgca tgaccactac tttctcgtcg cgatccagcc
    17101 agctacggtg aaccagccag ccggagggtt tatttaccgc aaccagccat tcatcctgat
    17161 agagtatttc cagcattagc tcgcatcatc cgcaaaaaga gcatccagtt tttccaactc
    17221 agccagaata agcgcgcgtt gcggatggtc cgtcgccagc gccatttcat aatagggtgc
    17281 aacagcaaaa gcgcccggta acggctggtt gttatttaac aaatcgtgca ttcgcgggat
    17341 cagcacccac tgcaaccact ccagtggttc catggtgtcc ataaagaacg gttgggtact
    17401 attaaattga tgcggctggg gttcatcgtt tcgccagtgc tgatgttcac gcagtaacgc
    17461 ttcaagcgtc tgcaactgga aacgaacgcg gtcatgagtg gtcataaagc aacttcaata
    17521 agaaaaactg ccgcgaagga tagcaggaat aaaaaaaggg agcactgtat tcacagtgct
    17581 cccggttcgt ttcgcagcat tccagctact tttgttgctc cctgctcatc cttgacaact
    17641 tttcctctgg ccttgcggcc aatcgttcat cctgaactat tgcttcctgc tcacaccacc
    17701 ccgatgtgat acttcatcct gaagtgtccc tggccttcct gacccaccga atcatcctga
    17761 ccggttctca ttctccatcc tggaggtgtc ctttaacgcg tcctgcgtca tcctcttcgc
    17821 ttcatccaga agcctttccc tgaaacacca tcctggtgtg tcctgcagaa gtgtcatcat
    17881 cctgatgttc acttcgttgt ctgactccct gtcgacgaag atagaatcgt ctttttcgcg
    17941 aagtcttaca aggcgcttac agacaatgcc ttaaggaaaa tttcatatga aagttaagaa
    18001 ttaacaatca acattttgat tttaaatgaa aataacgatt cttatctttt taagtattcg
    18061 cttatttcct ggcgatctcc tacagggtct gtaagagatc tctcacaacc agatggggta
    18121 tttggattac agaagaggct taagttgatt aaggaattcc gcaagatttg aggctaaatg
    18181 cgtccgttta cgtgttccca gcgtctcttt gcacacttca ccggagagat tacacaccga
    18241 gatgacctct aactcttctt ccagcgtcgc gataaatagc gtgggcggca gttttagcct
    18301 tttctgagtc accagatgac caatcagatt ttcctgcacc cgacggaagt cgtcttcact
    18361 ccaggtttgc agcaatgtca gtttgatatc gccaaactgc gcgtgcatat ccccggcaaa
    18421 ctgagtcgta taaaaggtat gaattgtggg ttgtatcaca atatcaaaag cgcgttcaac
    18481 cgcgtttaca ttttgttccc cggtaaacgg ctggggttgc cagtatacgg catcttcggt
    18541 agttgaaata atgcacggtg aaggaacacc gtacagttcc tcacttaacg gccagctttt
    18601 atgctcttca tgccacgcat cgcagtagcg tgcagtaaaa tctttcaggg cttgtgcggt
    18661 caaatcgtcc actaatttct ctcttcactt aaaccagata cacttgtcgt ttagtttatc
    18721 tggtttatga cggtgaaaca tgtcttctta tgcaaaccat caggcacttg cgggcctgac
    18781 tcttggaaaa tcaaccgatt accgggatac ctatgacgcc agcctactgc aaggcgttcc
    18841 acgcagcctg aatcgcgacc cgctgggtct gaaagcggat aacctgcctt ttcacggtac
    18901 ggatatctgg acgctgtatg aactttcctg gctgaatgcg aaaggtttgc cgcaggtcgc
    18961 tgtcggtcat gttgaacttg attacaccag cgtaaatctg attgagtcga agagttttaa
    19021 gctctatctc aacagtttta accagacgcg ttttaataac tgggatgagg tgcgccagac
    19081 gctggagcgc gacttaagca cttgcgctca gggtaagatt agcgtggcgt tatatcgtct
    19141 tgatgaactg gaaggccagc cgataggtca ttttaatggc acttgcattg atgaccagga
    19201 tatcactatc gataactatg aattcactac tgactatctg gagaatgcca cctgtggtga
    19261 aaaagtagtg gaagagacgc ttgtcagcca cctgctgaaa tcaaactgcc tgatcaccca
    19321 tcaaccagat tggggttcga tccaaattca gtatcgtgga cgccaaattg acagagaaaa
    19381 actgctgcgt tacctggtct cattccgtca tcacaacgag ttccacgaac agtgcgtgga
    19441 acgcatcttt aatgacctgt tacgcttctg ccagccagaa aaattgagcg tttacgcacg
    19501 ttatacccgt cgtggcggtc tggacattaa cccgtggcgc agtaatagcg attttgtccc
    19561 atcgaccaca agactggttc ggcaataaat tttttctcaa ttttgcgtgc tggattcacg
    19621 cagaaggttg tgaaaggaca tcaggcaggg ctattgtaat caaagggaat gacgatattc
    19681 gtcccataag gagtttttct tgattacaca tattagcccg cttggctcca tggatatgtt
    19741 gtcgcagctg gaagtggata tgcttaaacg caccgccagc agcgacctct atcaactgtt
    19801 tcgcaactgt tcacttgccg tactgaactc cggtagtttg accgataaca gcaaagaatt
    19861 gctgtctcgt tttgaaaatt tcgatattaa cgtcctgcgc cgtgaacgcg gcgtaaagct
    19921 ggaactgatt aatcccccgg aagaggcttt tgtcgatggg cgaattattc gcgcgttgca
    19981 ggccaacttg ttcgcggttc tgcgtgacat tctcttcgtt tacgggcaaa tccataacac
    20041 cgttcgtttt cccaacctga atctcgacaa ctccgtccac atcactaacc tggtcttttc
    20101 catcttgcgt aacgctcgcg cactgcatgt cggtgaagcg ccaaatatgg tggtctgctg
    20161 gggcggacac tcaattaacg aaaacgagta tttgtatgcc cgtcgcgtcg ggaatcagct
    20221 gggcctgcgt gagctgaata tctgcaccgg ctgtggtccg ggagcgatgg aagcgccgat
    20281 gaaaggtgct gcggtcggac acgcacagca gcgttacaaa gacagtcgtt ttattggtat
    20341 gacagagccg tcgattatcg ccgctgaacc gcctaacccg ctggtcaacg aattgatcat
    20401 catgccagat atcgaaaaac gtctggaagc gtttgtccgt atcgctcacg gcatcattat
    20461 cttccctggc ggtgtgggta cggcagaaga gttgctgtat ttgctgggaa ttttaatgaa
    20521 cccggccaac aaagatcagg ttttaccatt gttcctcacc ggcccgaaag agagcgccga
    20581 ctacttccgc gtactggacg agtttgtcgt gcatacgttg ggtgaaaacg cgcgccgcca
    20641 ttaccgcatc atcattgatg acgccgctga agtcgctcgt cagatgaaaa aatcgatgcc
    20701 gctggtgaaa gaaaatcgcc gcgacacagg cgatgcctac agctttaact ggtcaatgcg
    20761 cattgcgcca gatttgcaaa tgccgtttga gccgtctcac gagaatatgg ctaatctgaa
    20821 gctttacccg gatcaacctg ttgaagtgct ggctgccgat ctgcgccgtg cgttctccgg
    20881 tattgtggcg ggtaacgtaa aagaagtcgg tattcgcgcc attgaagagt ttggtcctta
    20941 caaaatcaac ggcgataaag agattatgcg tcgtatggac gacctgctac agggttttgt
    21001 tgcccagcat cgtatgaagt tgccaggctc agcctacatc ccttgctacg aaatctgcac
    21061 gtaatctccc tcccccggtg acgtttgttg ccgggttttc tcgtttttgg tcacataccc
    21121 atcaatttat ttcatttgtt atatgaatgt ttcttaccac cctcacgcga taaatatcat
    21181 tgcagttaat atgtcataca atttatgttg taacgcaaac gtttccctat tttcataaac
    21241 cacattttat cgctatgaaa aagaaattat cgccatgatt aactaaaagt attgattttt
    21301 tcagttcaac ctacatatat tgcgcgcccc ggaagaagtc aaatgtcgtt taatgggcaa
    21361 atattgccct taaattctct tttacttttg atttacagag taaagcgttg ggataatcta
    21421 tcttccaagt agattattgt atttgggatc aagatcactg atagatacat aacttgtgtg
    21481 tatctttccg ccctcaaatt attacggcgg taaatgatta agccatcgcc gatagacaga
    21541 tttcattttt acggtcaggc accttcccga gctgaactgg ctaaaagctg aattatttgc
    21601 attcctccag gagaaataga tggaaacgac tcaaaccagc acgattgcgt cgaaagactc
    21661 tcgtagtgcc tggcgcaaga cagacaccat gtggatgctg ggcctttacg gcacggcaat
    21721 cggcgcgggc gtgctgttcc tgccaatcaa cgccggtgtt ggcggtatga tcccgctgat
    21781 catcatggct atccttgcgt tcccgatgac attttttgct caccgcggcc tgactcgctt
    21841 cgtactgtct ggtaaaaacc caggcgaaga catcaccgag gttgtagaag aacactttgg
    21901 tattggcgca ggtaaactga ttaccctgct ctacttcttc gctatctacc cgatcctgct
    21961 ggtttatagc gtggcaatca ccaatacagt tgaaagcttc atgtctcacc agctgggtat
    22021 gacaccaccg ccgcgtgcga ttctgtcgct gatcctgatc gtgggtatga tgaccatcgt
    22081 tcgcttcggt gagcagatga tcgttaaagc aatgagtatt ctggtattcc cgtttgttgg
    22141 cgtactgatg ctgctggctc tgtacctgat cccgcagtgg aacggcgcag cactggaaac
    22201 gctgtctctg gacactgcat ctgcaaccgg aaacggtctg tggatgaccc tgtggctggc
    22261 aattccggtt atggtgttct ctttcaacca ctctccgatc atctcttctt tcgccgttgc
    22321 gaagcgtgaa gagtacggcg atatggcaga acagaaatgc tcgaagatcc tggcattcgc
    22381 acacatcatg atggtgctga ccgtaatgtt cttcgtcttc agctgtgtac tgagcctgac
    22441 tccggcagac ctggctgcgg ctaaagagca gaacatctcg attctgtctt acctggctaa
    22501 ccactttaac gcaccggtta tcgcgtggat ggctccgatt atcgcgatta tcgctatcac
    22561 caaatccttc ctcggccact acctgggcgc acgtgaaggc ttcaacggta tggtgattaa
    22621 atctctgcgt ggtaaaggta agtctatcga aatcaacaag ctgaaccgta tcactgcgct
    22681 gttcatgctg gtaacgacct ggattgttgc caccctgaac ccgagcatcc tgggtatgat
    22741 tgaaaccctg ggcggcccaa tcatcgcgat gatcctgttc ctgatgccga tgtacgcaat
    22801 tcagaaagta ccggcaatgc gtaagtacag cggtcacatc agcaacgtat tcgttgtcgt
    22861 gatgggtctg attgcaatct ccgcaatctt ctactctctg ttcagctaag tcctttcgcg
    22921 ccgctttcgg gcggcgcttc ctccgtttta acgcgatgta tttcctatga ttagcgtatt
    22981 cgatattttc aaaatcggca ttggcccttc cagttctcat accgttggac caatgaaagc
    23041 gggtaaacaa tttaccgacg atctgattgc ccgtaatctg cttaaagacg tgacccgcgt
    23101 ggtggttgac gtgtacggct cgctctctct gaccggtaaa ggccaccaca ctgatatcgc
    23161 cattattatg ggcctggcgg gtaacctgcc ggataccgtg gatatcgatt ccatccctgg
    23221 ttttattcag gatgtgaata ctcatggtcg cctgatgctg gcaaacggtc agcatgaagt
    23281 ggagttcccg gttgatcagt gcatgaactt ccatgcagac aacctttctc tgcatgaaaa
    23341 cggtatgcgc attaccgcgc tggcgggcga taaagtcgtt tacagccaga cttactactc
    23401 tattggcggt ggctttatcg ttgatgaaga gcattttggc cagcagaata gcgcaccggt
    23461 tgaagttcct tatccgtaca gttcagcagc cgatctgcaa aaacattgtc aggaaaccgg
    23521 gctgtcactc tctggcctga tgatgaaaaa tgagctggcg ctgcacagca aagaagagct
    23581 ggaacagcac ctggcgaacg tatgggaagt catgcgtggc ggtattgagc gcggtatttc
    23641 caccgaaggc gtgttgcctg gcaaactgcg cgttccacgc cgtgctgcgg cactacgccg
    23701 gatgctggtc agccaggata aaaccaccac tgacccgatg gcggttgttg actggatcaa
    23761 catgtttgca ctggcagtga acgaagagaa cgctgctggc ggacgcgtgg tgactgcgcc
    23821 gactaacggt gcgtgcggga ttatcccggc agtgctggcg tactacgaca agttcatccg
    23881 cgaagtgaac gctaactcac tggctcgtta cctgctggta gccagtgcca ttggttctct
    23941 ttataagatg aacgcgtcaa tttctggtgc tgaagtcggc tgccagggtg aagttggtgt
    24001 ggcgtgctca atggcggcgg ctggtctggc agaactgtta ggtgcaagcc cggcgcaggt
    24061 gtgcatcgcg gcggaaatcg ccatggagca caacctcggt ctgacgtgtg acccggtcgc
    24121 cggacaggtc caggtgccat gcatcgagcg taacgccatt gcggcagtaa aagcggtgaa
    24181 cgccgcacgt atggcgctac gccgtaccag cgagccgcgc gtctgcctcg ataaagttat
    24241 cgaaaccatg tacgaaacag gtaaagatat gaacgccaag taccgcgaaa cctctcgcgg
    24301 cggcctggca atgaagatcg ttgcctgcga ttaatcgctc tccagaggcc tcgttttgcg
    24361 aggcctcttc ccgatttctc atccagccgt agcctgttcc ggcatcgaat gttaccctta
    24421 tcgcctgatc tttaaggggg ttatcgtggc tgttcatttg cttattgtcg atgcactgaa
    24481 tcttattcgt cgcattcatg ccgttcaggg atcgccctgt gtcgaaacct gccagcatgc
    24541 gctcgatcag ctcattatgc acagccagcc aacccacgcg gtcgccgttt ttgatgatga
    24601 aaaccgcagt agcggctggc gtcatcagcg tttaccagat tacaaagcgg gtcgaccgcc
    24661 aatgccggaa gagttgcacg acgagatgcc tgcattacgc gccgcctttg agcaacgcgg
    24721 cgtcccgtgc tggtcaacca gcggcaacga agccgatgac ttagccgcca cgctggcggt
    24781 caaagtgaca caggccgggc atcaggcaac gattgtttcg acagataaag gctactgtca
    24841 gttactttca ccgacattac gtattcgtga ttacttccag aaacgttggc tggatgcgcc
    24901 atttatcgat aaagaatttg gcgttcaacc gcagcagttg cccgattact ggggacttgc
    24961 ggggatcagc agttcaaagg taccgggtgt tgcgggaatc ggaccaaaaa gcgccacgca
    25021 gctgctggtc gagtttcaga gtctggaagg gatatatgag aatctggatg cggttgccga
    25081 aaagtggcgc aaaaaattag aaacccataa agagatggcg tttctgtgcc gcgatattgc
    25141 ccgcttacaa accgatttgc atatcgacgg caatttacag caattgcggt tggtacggta
    25201 acggcgagcc ggatgcaaac gattatcaac acattgaatt taatgcactt atataccgga
    25261 taagacgcgg ctagcgtcgt atccggcatt atcacatcag cgcatttacc aggcggtatg
    25321 gtaaagctct acaatatcct caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc
    25381 atccagtgcc gcctgcgcca gtgccggaat gtcttccttg cgtacaccaa catcacgcaa
    25441 atgtggcgga ataccgacat cacggttgag agcaaacacc gcttcaacag cggcattacg
    25501 cgcctcttcc aggctcatac cttccacttt cacgcccata acgcgcgcga tatcgcggta
    25561 cttctcaccg gtaaagtcag cgttatagcg catgacatgc ggtaacagga tggcgttcgc
    25621 aacaccgtgt ggagtgttgt aaaacgcgcc cagtggatgc gccataccat gcaccaaccc
    25681 taacccaaca ttcgagaagc ccatacccgc aacatactgc ccgagcgcca tttcttctcc
    25741 ggcatcctta tcaccagcaa ccgatcctcg cagcgcccca gcaatgattt caatcgcttt
    25801 aatgtgcagt gcatcggtta gcgcccacgc gccacgggta atatacccct caatagcatg
    25861 agtgagcgca tcgacacccg tcgcagcttt cagcgctgga ggcataccat ccatcatgtc
    25921 agcgtcaata aacgccacct gcgggatatc atgcggatca acgcaaacaa acttgcgccg
    25981 tttctcttcg tcagtgatca cgtagttaat ggtcacttct gccgcagtac ctgctgtggt
    26041 aggaattgcc agaatcggta cactgggttt attggtcggg gaaagccctt ccaggctacg
    26101 cacatcggca aactccgggt tgttgctgat aatgccaatc gctttacaag tatcctgtgg
    26161 agaaccacca ccaatagcga tcaggtaatc cgcgccgcta ttctggaata caccgagccc
    26221 ttctttgacg acagtaattg ttgggttggg cactacgccg tcgtaaatcg cccatgccag
    26281 ccctgcagca tccatcttat cggtcacttt cgccaccacg ccgcattgca ccagcgtttt
    26341 atcggtgacg atcagcgcct tctgataacc acggcgtttc acctcatcgg ttaaagcccc
    26401 aacagcaccc cgaccaaacc atgccgtttc gttcagaatc attctgttag ccatcatcct
    26461 tctccttgtt gctttacgaa attactcttc aatccgtaac ccataggttt tgaatttctc
    26521 cagcactacg gcaatctctt catcgctcag cactggcacc gggtccgtaa tcgccagggt
    26581 cgtcacgtaa agttgcgcca gcacttcaac ttcatgcgcc agccataacg ctttttccag
    26641 attcacctca caagcgataa gcccatgatg ttgtaacaaa gttgccttac gatttttgag
    26701 agccagcgca acatgttcag aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg
    26761 aatagaatta ccacctgccg ccgcaatcat gtagtgaata gcggggatcg gtcggttaag
    26821 aatggaaact gctgtgcaat gaacggcatg attgtgaaca accgcgttgg catccggtct
    26881 gctttgatag gccgccatat ggaaacgcca ttcgcttgag gggagctttc cttcctcatg
    26941 tttaccgttg ccatcaataa agacaatatg cgactccgtc agtttttcat atggaatgcc
    27001 tgtaggcgta atcagcatcc catcctgata acgtacactg acgttccccg ctgtcccctg
    27061 gttcagtccc aggcgggtca tttccaggca agtgtcaata atctgacgag caagtttatt
    27121 tcgttccatt agctacctct ctctgattca aaacagggca ataatgttgt tcctttcaca
    27181 ctattgaatt agccgtttaa ttacccacca tcttcttcct gattaacaag aaagaaattc
    27241 acaagcttat attttgtgac ctagctcaac taatcacagt aaataactgc aagttctctt
    27301 tttataaccc cattaaaaat gaccgctctt aaaaatattt atcaaaacgg tcatttttct
    27361 attcacccaa gccaggaatg cccgttttcg gcataaacaa ttaatacagt catctggttt
    27421 gtgtttttta tgatttattt tctgaaacgg gcatgaaatt tcgattatta aagtgatggt
    27481 agtcacataa agtcaccttc cagctaataa gtgtgaccgc cgtcatatta cagagcgttt
    27541 tttatttgaa aatgaatcca tgagttcatt tcagacaggc aaatattcac tgatatgaag
    27601 cccgaactcg ctggttttgc acttttgaaa acataaccga ttacgtgctt aagcttctga
    27661 acctaagagg atgctatggg aaacacatca atacaaacgc agagttaccg tgcggtagat
    27721 aaagatgcag ggcaaagcag aagttacatt attccattcg cgctgctgtg ctcactgttt
    27781 tttctttggg cggtagccaa taaccttaac gacattttat tacctcaatt ccagcaggct
    27841 tttacgctga caaatttcca ggctggcctg atccaatcgg ccttttactt tggttatttc
    27901 attatcccaa ttcctgctgg gatattgatg aaaaaactca gttataaagc aggaattatt
    27961 actgggttat ttttatatgc cctgggcgct gcattattct ggcccgctgc agaaataatg
    28021 aactacacct tatttttagt tggcctattt attattgcag ccggattagg ttgtctggaa
    28081 actgccgcaa acccttttgt tacggtatta gggccggaaa gcagtggtca cttccgctta
    28141 aatcttgcgc aaacatttaa ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt
    28201 cttattttgt ctaacgtgcc acatcaatcg caagacgttc tcgataaaat gtctccagag
    28261 caattgagtg cgtataaaca cagcctggta ttatcggtac agacacctta tatgatcatc
    28321 gtggctatcg tgttactggt cgccctgctg atcatgctga cgaaattccc ggcattgcag
    28381 agtgataatc acagtgacgc caaacaagga tcgttctccg catcgctttc tcgcctagcg
    28441 cgtattcgcc actggcgctg ggcagtatta gcgcaattct gctacgttgg cgcacaaacg
    28501 gcctgctgga gctatttgat tcgctacgct gtagaagaaa ttctaggtat gactgcaggc
    28561 tttgccgcta actatttaac cggaaccatg gtgtgcttct ttattggtcg tttcaccggt
    28621 acctggctca tcagtcgctt cgcaccacac aaagtcctgg ccgcctacgc attaatcgct
    28681 atggcactgt gcctgatctc agccttcgct ggcggtcatg tgggcttaat agccctgact
    28741 ttatgcagcg cctttatgtc gattcagtac ccaacaatct tctcgctggg cattaagaat
    28801 ctcggccagg acaccaaata cggttcgtcc ttcatcgtta tgaccattat tggcggcggt
    28861 attgtcactc cggtcatggg ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa
    28921 ctgatcccca cactctgctt cgcggtcatc tttatctttg cccgtttccg ctctcaaacg
    28981 gcaactaact gaacatattt tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct
    29041 taccgaaaat tggtatccgc ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc
    29101 ttgaagaaca aacaatgaat atggcgaaag ctacggccgc actgctgacc gagaaactgc
    29161 gccatgcctg cggagctgcc gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg
    29221 ctgaagccgc tgcttgcgaa gaaaaattca gcagccagaa tgtaggcctc accattacgg
    29281 taacgccttg ctggtgctat ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag
    29341 ccatttgggg ctttaacggc actgaacgtc ccggcgctgt ttacctggca gcggctctgg
    29401 cagctcacag ccagaaaggc atcccggcat tctccattta cggtcatgac gttcaggatg
    29461 ccgatgacac atcgattcct gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg
    29521 gtttggccgt cgccagcatg aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg
    29581 gtatcgccgg ttctattgtt gatcacaact tctttgaatc ctggctggga atgaaagtcc
    29641 aggcggtgga tatgaccgaa ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg
    29701 aactggaaat ggcactgacc tgggctgata aaaacttccg ctatggcgaa gatgaaaata
    29761 acaaacagta tcaacgcaat gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga
    29821 tggcgatgtg tatccgcgac atgatgcaag gcaacagcaa actggccgat attggtcgcg
    29881 tggaagaatc acttggctac aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga
    29941 ccgatcaata tcccaatggt gacaccgccg aagcgatcct caacagttca tttgactgga
    30001 atggcgtgcg cgaacccttt gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa
    30061 tgctaatggg tcaccagctc accggcaccg ctcaggtatt tgccgatgtg cgtacctact
    30121 ggtcaccaga agcaattgag cgtgtaacgg ggcataaact cgatggactg gcagaacacg
    30181 gcatcatcca tttgatcaac tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg
    30241 acagcgaagg taacccgacg atgaagccac actgggaaat ctctcagcaa gaggctgacg
    30301 cttgcctcgc cgctaccgaa tggtgcccgg cgatccacga atacttccgt ggcggcggtt
    30361 actcttcccg cttccttacc gaaggcggcg tcccgttcac catgactcgt gtcaacatca
    30421 tcaaaggcct gggatcggta ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg
    30481 atgtgcatga catcctcaac aaacgcacca actcaacctg gccaaccacc tggtttgcac
    30541 cgcgcctcac cggtaaaggg ccgtttacgg atgtgtactc ggtaatggcg aactggggcg
    30601 ctaaccatgg ggttctgacc atcggccacg ttggcgcaga ctttatcact ctcgcctcca
    30661 tgctgcgtat cccggtatgt atgcacaacg ttgaagagac caaagtgtat cgtccttctg
    30721 cctgggctgc gcacggcatg gatattgaag gccaggatta ccgcgcttgc cagaactacg
    30781 gtccgttgta caagcgttaa tacattactc cctgatgtga tgcccggtcg ctccggctac
    30841 cgggcctgaa caagcaagag tggttagccg gataagcaat gttatccggc tatattgcag
    30901 gagcgattat gaaacaagaa gttatcctgg tactcgactg tggcgcgacc aatgtcaggg
    30961 ccatcgcggt taatcggcag ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg
    31021 atatcgcgat ggaaaacaac acctggcacc agtggtcttt agacgccatt ttgcaacgct
    31081 ttgctgattg ctgtcggcaa atcaatagtg aactgactga atgccacatc cgcggtatcg
    31141 ccgtcaccac ctttggtgtg gatggcgctc tggtagataa gcaaggcaat ctgctctatc
    31201 cgatcattag ctggaaatgt ccgcgaacag cagcggttat ggacaatatt gaacagttga
    31261 tctccgcaca gcggttgcag gctatttctg gcgtcggagc ctttagtttc aatacgttat
    31321 ataagttggt gtggttgaaa gaaaatcatc cacaactgct ggaacgcgcg cacgcctggc
    31381 tctttatttc gtcgctgatt aaccaccgtt taaccggcga attcactact gatatcacga
    31441 tggccggaac cagccagatg ctggatatcc agcaacgcga tttcagtccg caaattttac
    31501 aagccaccgg tattccacgc cgactcttcc ctcgtctggt ggaagcgggt gaacagattg
    31561 gtacgctaca gaacagcgcc gcagcaatgc tcggcttacc cgttggcata ccggtgattt
    31621 ccgcaggtca cgatacccag ttcgcccttt ttggcgctgg tgccgaccaa aatgaacccg
    31681 tgctctcttc cggtacatgg gaaattttaa tggtccgtag cgcccaggtt gatacttcgc
    31741 tgttaagtca gtacgccgat tctacctgcg aactggatag ccaggcaggg ttgtataacc
    31801 caggtatgca atggctggca tccggcgtgc tggaatgggt gagaaaactg ttctggacgg
    31861 ctgaaacacc ctggcaaatg ttgattgaag aagctcgtct gatcgcacct ggcgcggatg
    31921 gtgtgaaaat gcagtgtgat ttattgtcgt gtcagaacgc tggctggcaa ggagtgacgc
    31981 ttaataccac gcgggggcat ttctatcgcg cggcgctgga agggttaact gcgcaattac
    32041 agcgcaatct acagatgctg gaaaaaatcg ggcactttaa agcctctgaa ttattgttag
    32101 tcggtggagg aagtcgcaac acattgtgga atcagattaa agccaatatg cttgatattc
    32161 cggtaaaagt tctcgacgat gccgaaacga ccgtcgcagg agctgcgctg ttcggttggt
    32221 atggcgtagg ggaatttaac agcccggaag aagcccgtgc acagattcat tatcagtacc
    32281 gttatttcta cccgcaaact gaacctgaat ttatagagga agtgtgaaat gctgaaaaca
    32341 atttcgccgt taatttctcc cgaactattg aaagtgctgg cagagatggg acatggagat
    32401 gaaattattt tttccgatgc tcactttccc gcccattcga tgggaccgca ggtgatccgc
    32461 gctgatggcc tgttggtgag cgacttgctc caagcgatta tcccattatt tgagctggac
    32521 agttatgccc cgccgctggt gatgatggca gcggtagaag gtgacactct cgatcctgaa
    32581 gtagaacgac gttaccgtaa tgcgctttca ctacaagccc cgtgtcctga catcatccgc
    32641 atcaatcgtt ttgcgtttta tgaacgggcg caaaaagcct ttgcgatcgt tatcacaggc
    32701 gaacgagcga agtacgggaa tattctttta aaaaaagggg taacaccgta atctcatatc
    32761 ggtacgcccg catgacgcgg gcggttatcg aatgatgggg tgaaaaatat gaaagcggca
    32821 cgccagcaag cgatagtcga cctgctgctg aaccatacca gcctgaccac ggaagctctc
    32881 tctgaacagc taaaggtcag taaagaaacc attcgtcgcg atctcaatga attacagacg
    32941 cagggtaaaa ttctgcgcaa tcatggacgc gctaaatata tccaccgtca aaatcaagac
    33001 agtggcgatc cctttcacat caggctgaaa agccattatg cgcataaagc agatatcgcg
    33061 cgcgaggcgc tcgcgtggat tgaagaaggg atggtgatag ccttagacgc cagttcaact
    33121 tgctggtatc tggcacgcca gttgcctgac atcaacattc aggtcttcac caatagccat
    33181 ccgatttgcc atgaactcgg taaacgcgaa cgcattcaac tgatcagttc cggcggcaca
    33241 cttgagcgca aatatggctg ttacgtcaat ccctcgctga tttcccaact taaatcgctg
    33301 gaaatcgatc tgtttatttt ttcttgtgaa gggatcgata gcagcggcgc actgtgggac
    33361 tccaatgcga tcaacgctga ttacaaatcg atgctattaa aacgtgccgc gcaatcgttg
    33421 ttattgattg ataaaagtaa atttaatcgt tcaggggaag cccgcatcgg gcatctggat
    33481 gaggtaacgc acattatttc tgatgagcgc caggttgcaa cttctttggt aacagcctga
    33541 cggaaagggt agcaggccga agacgacgcc ccggccttgc ctgttatcgc tcgtcgcgac
    33601 gaccacccac cgcagcccag atgcggcgga cgtgcaccgt cacttcttcg cgatcgtgat
    33661 acaactgccg cgcctgaatc tgagcattta tgccatgttc atcaagctgt gcctgaatat
    33721 acgccagatt gtgtgatact tcttcgtagc gttttttcat cggcagtttg agattgaaaa
    33781 tggtttcacg gcaccagcca ttaaccagcc actgcgccat caatgccgca actttagccg
    33841 gtttttcaac catatcgcat accatccagg agatattgct gcgtgtcgga cggaatttga
    33901 aaccgtcttc ccgcagccac gtcacctgcc cggtatccat cagactttgc gccatcgggc
    33961 cgttgtcgac ggaataaacc cacatgttgc gcttcaccag ttggtaggtc cagccgccag
    34021 ggcaagcgcc taaatccacc gcccacatcc cgttcgccag gcgttcatcc cactcatccg
    34081 caggaataaa cacatgaaat gcctcttcca gtttgagcgt ggaacgactc ggcgcctctg
    34141 ccggaaattt caggcgcgga atgcccatat agaacggcga attattgttg ctgtatgagt
    34201 aaccggtata gcagcagcct ggtgcaataa agaatacatg cacaaccgga cgcttcggcg
    34261 tttcatagtt cgccagcacc ccggcatcgc gcaaggcagc gcgtagcggc acggtgaatt
    34321 tacggcagaa tttcagcaac tctttgcttt cgttggtatc ggcaacttca acacgcagtt
    34381 caccgccctt ctctactacg ccctgcaaca tgtcgacaat gggggtaata cgatcttctg
    34441 gcggcaaatg ctgcaagagt tcccccacca caaaccactg gcgggcaaaa attaatgaac
    34501 tgaacggcag ctcacggatt aacttatcgc catcatcagg ttgataacat tcataaatga
    34561 cataacccgc attctctttc acgcgggcaa aaccgaaaat ttcccgctgg ccggctttat
    34621 cggtaatttc tgcggcgcac tctttttcaa agcccggacg gcacagcaat acaaccttat
    34681 tcatgactta cgcccttgca ctttaaacgg atagcaccaa ctaacattaa cgcccagccc
    34741 gcgaggaagc tcacgccgcc aaccggtgtg acaaatgccc acaaacgcag atgggacagc
    34801 gccaggcaat aaaggctgcc gctgaacaac accgtgccta acgcgaggaa aacgctactc
    34861 cagtaaaacc agatactgat gcgacgctgc attgccaccg ccagacctaa gatcgccagc
    34921 gtatgaaacg cctggtattc gaggccggtc tggatccagc ccatctcaac ggcccccatg
    34981 gttttactta acacatgcgc gccaaaagcg cccagagcca caaaaatgaa gccgctaatg
    35041 gcggcgaaaa tcagcataaa acggctggtc atggtcgtac cctacgtaaa ttattgttca
    35101 taacgaaagc ggaatttttc ttgttcagca gcggctttcg ccaggatcca ttggcgaaag
    35161 gcggctattt tacccagttc tgcctggctg tcatgacaaa ccagataaaa agcgttttta
    35221 ctgaccagaa catcattaaa cgggcaaaca agacgtccgg cctcgatttc agattgcgcc
    35281 atcacgttat ttgccagcgc cactccctgc ccgtgaatag ccgcttgcag caccatggcg
    35341 ctatgactaa aaattggccc ttgctgaacg ttgatatgat ttaaccccaa ctgtcgggta
    35401 tatgtctgcc agtcacggcg tgacgcatca tgtaataacg tatgtttagc cagatcttcc
    35461 ggggtcttca agggtttttc gcctgtcagc agtagcggcg aacacaccgg caataaatat
    35521 tcggcgtaca gtttttccac ccgtagcccc ggccagttgc cccgaccata aaatatcgcc
    35581 acatcaacat catccgccag cttatcttcc tgacgatcaa ccgcctggat tcgaacgtca
    35641 attcccggat aagctgaatt aaagctggaa agtcgcggaa ccaaccaatg aatggcgaaa
    35701 ctggggagta aactgaccgt caacgccccc ttggcgctac gggcctggag tttacgcgtc
    35761 gcttcggtta attgcgaaaa tatctctttg atatcgagga aatagctttg cccttcctcg
    35821 gtcagcagga gtgaacgatt acggcggcgg aacagtttta gccccaaaaa atcctcaaga
    35881 gacttgattt gatgacttac tgcggcctgg gtcacgaaaa gctcttctgc tgcgcgagtg
    35941 aaacttaaat ggcgtgctgc ggcatcaaaa actcgtaagg catttagcgg tggtaatcgt
    36001 ttagacatgg cttttaaact ttgatgttaa attaatttaa caattagatc acactgtgta
    36061 acctattagt ttttttaatc tgagccatta taaattgtcc gttgagcttc taccagcaaa
    36121 tacctatagt ggcggcactt cctgagccgg aacgaaaagt tttatcggaa tgcgtgttct
    36181 ggtgaacttt tggcttacgg ttgtgatgtt gtgttgttgt gtttgcaatt ggtctgcgat
    36241 tcagaccatg gtagcaaagc tacctttttt cacttcctgt acatttaccc tgtctgtcca
    36301 tagtgattaa tgtagcaccg cctaattgcg gtgctttttt ttaccttgcg atcgcgaatt
    36361 actgatccag ttcgaccatc tctttcacgt cagtacggtt gatctgctgt ttgttgccat
    36421 tagcgtcttt atacgaaatc ataccggtat cgttatcagt ctgtggtttg ccgtcagaga
    36481 cgatggtacg tccgtcattg gtgtgcatca catagttcga accggaacag gcgctcaggg
    36541 caaaagtcag catacaggca gaaataattg cggcagtctt tttcatcatc ttctccttaa
    36601 agcgattaat aaatcttaat agtattgatt aacaggctaa aattaacgcc taacactatt
    36661 cagcatatgt tacttacgcg gcttcgccag gatatccaga taattctgat ggttagcact
    36721 ctccttgtat caaagtgaat tttgcgtcac gatcggtgca tcaagccgag gagtaccatg
    36781 aacgttttta atcccgcgca gtttcgcgcc cagtttcccg cactacagga tgcgggcgtc
    36841 tatctcgata gcgccgcgac cgcgcttaaa cctgaagccg tggttgaagc cactcgacag
    36901 ttttacagcc tgagcgccgg aaacgtccat cgcagccagt ttgccgaagc ccaacgcctg
    36961 accgcgcgtt atgaagctgc acgagagaaa gtggcgcaat tactgaatgc accggatgat
    37021 aaaactatcg tctggacgcg cggcaccact gaatccatca acatggtggc acaatgctat
    37081 gcgcgtccgc gtctgcaacc gggcgatgag attattgtca gcgtggcaga acaccacgct
    37141 aacctcgtcc cctggctgat ggtcgcccaa caaactggag ccaaagtggt gaaattgccg
    37201 cttaatgcgc agcgactgcc ggatgtcgat ttgttgccag aactgattac tccccgtagt
    37261 cggattctgg cgttgggtca gatgtcgaac gttactggcg gttgcccgga tctggcgcga
    37321 gcgattacct ttgctcattc agccgggatg gtggtgatgg ttgatggtgc tcagggggcg
    37381 gtgcatttcc ccgcagatgt tcagcaacta gatatcgatt tctatgcttt ctctggtcac
    37441 aaactgtatg gcccgacggg tatcggcgtg ctgtatggca aatcagaact gctggaagcg
    37501 atgtcgccct ggctgggtgg cggcaaaatg attcacgaag tgagttttga cggcttcacg
    37561 actcaatctg cgccgtggaa actggaagca ggaacgccaa atgtggctgg tgtcatagga
    37621 ttaagcgcgg cgctggaatg gctggcagat tacgatatca accaggcaga aagttggagt
    37681 cgcagcctgg cgacgctggc ggaagatgcg ctggcaaaac gtcctggctt ccgctcattc
    37741 cgctgccagg attccagcct gctggccttt gattttgctg gcgttcatca cagcgatatg
    37801 gtgacgctgc tggcggagta cggtattgcc ctgcgggccg ggcaacattg cgctcagccg
    37861 ctactggcag aattaggcgt aaccggcaca ctgcgcgcct cttttgcgcc atataataca
    37921 aagagtgatg tggatgcgct ggtgaatgcc gttgaccgcg cgctggaatt attggtggat
    37981 taatgacgaa cccgcaattc gccggacatc cgttcggcac aaccgtaacc gcagaaacgt
    38041 tacgcaatac cttcgcaccg ttgtcgcaat gggaagataa atatcgccag ttgatcatgc
    38101 tggggaaaca gcttccggct ttgccagacg agttaaaagc gcaggctaaa gagattgccg
    38161 gatgcgaaaa ccgcgtctgg ctgggatata ccgtgactga aaacggtaaa atgcacttct
    38221 ttggcgatag cgaagggcgc attgtgcgcg gcctgctggc ggtgctgttg actgccgttg
    38281 aggggaaaac cgccgccgaa ttgcaggcac agtcgccgct ggcattgttt gatgagctgg
    38341 gattgcgtgc gcaacttagc gcctcacgca gccaggggtt aaatgcgtta agcgaggcga
    38401 ttatcgctgc gacgaagcag gtttaagcat agaaccgggt cggataagac gttcgcgtct
    38461 catccgacct gattgtttaa ccctgacgcg ccgctttcgc catcatcttc ttcagcgcat
    38521 gagaaaccgc aacaaaccca aaggtggcgg tcaccatcgt tgccgcgcca aatccagatg
    38581 cacaatccat ccgcttcggc ccttctgccg tggctttcat cgcacacaca gtaccgtctg
    38641 actgcgggta caccagcgct tcagtagaaa acacgcaatc gacgccgagc ttacctttac
    38701 tgtttttcac tacgccaaaa tcgcttttca ggcgctcgcg caacttcgcc gccagcggat
    38761 cctgaatcgt tttcgccaga tcggtaacct gaatctgcgt cgggtcaatc tgcccacccg
    38821 cgccaccggt cgtgaccagc gggattttat tgcgccgaca ataagcaatc agcgccgctt
    38881 tgggccgtac actatcaatg gcatcaatca cgtacgaata acccacgctc atatactgcg
    38941 ctacgttatc cggcgtcacg aaatcatcca ccaccgttac acggcactcc gggttaatct
    39001 ggcgaatacg ctccgccatg acttccgctt ttgccagccc gacgttatcg cgcagagcgt
    39061 gaatttgccg attggtattg gtgacgcaca catcatccat atcgataagc gtgattgcgc
    39121 caatccccgt gcgcgccagc gcttccgccg cccaggaacc gacaccaccg ataccaacca
    39181 cacaaatatg cgcgtcagca aacagttgca acgctttttc accatacaga cgcgctgtgc
    39241 caccaaaacg ctgacgccat gcatcactaa ttaccacaga catagcacct cagaattaaa
    39301 aagggtgagg ttatccctca ccctgtcgta tccgtaaaaa cggtatacag aataacacat
    39361 cagccgctaa agacgttacc tgcgcccggg gcggttttca gcacccagac acgtccatag
    39421 tggttgtacc aacctgcgcg gtgtccggct tccggcccga tcccttgata gatatcgaag
    39481 tgttggcctt tgattgcacc accgacatcc agcgccacca tcagacgcag ttcgtactga
    39541 ccattaaatt tgccgttatt atccagcaac ggcacttctg ccagcaaggt agtacctggc
    39601 ggaataatgg aacgatcaga ggcaactgac gcgcgaccaa ccagcggcac cgcacttgcc
    39661 cctttcaccg gtgcaaaaga ttgcggttta aagaagacga aagacgggtt ctgttccagc
    39721 agctcgcgaa cctcggcttc actgtgtgtt tcgccccagt gacgaatcgc ctgcatcgac
    39781 atatcttctt ttttcacttc gccacggtcg atcagcacct taccaatgct gcgataggca
    39841 tgaccgtttt tccctgcata gctgaaaaag ttaagcggac tgccatcacc aaagtcgata
    39901 tacccactac cctgcacatc cataatgaag ttatccatca gggagttact gtaagcgaga
    39961 atatatttat cactcaatgc ccccgcgtag atctccgcac gagacggcag acgaccacgt
    40021 tttggcggca tacggtaaat aggatactgg aactcgccct ggcgggtatg gcgcgcctga
    40081 attaccggcg tgtaatagcc tgtaaactgc acgttaccat agttgtcggc accttccatc
    40141 tgccaggcat caatgccgaa ctggcgcata ttgcgggtat caccgcctgc gcgcagccac
    40201 tcttgcaccg cgttataaac attactctgg ttgccataca gacgcggtga cgaattacgg
    40261 atatggttaa tttgctcggc aaaatcaccg gcgttaatcg gcgcgccaac ggcatctggc
    40321 tggttcacca gagagaaagg ctgggtaaat ttcccgtctt tatattgctg tccgcgatcg
    40381 gttggtttgg aagagcaggc ggcaagcatt gccacaaccg tgcccataag aaggtacttt
    40441 acccaacgtc ctttcattgt tctcttcttc aggttaaaaa ataaggcgca acgaagataa
    40501 caaaccgccg cgtttaatga aatgagcagc ctctccctga cgcaaatttt gcacaaaaaa
    40561 taggctttag tgatttgttt ttgttcaaaa tcatgccaga tccgtgatcg gggtaaaaaa
    40621 aaggttgcat gaaaacgcga gcggagtata gtgcgcatcc acggaggcgg ggtggagcag
    40681 cctggtagct cgtcgggctc ataacccgaa ggtcgtcggt tcaaatccgg cccccgcaac
    40741 caattattga acaccctaac gggtgttttt ttgtttctac tctcccataa aaaaagcgcc
    40801 attcagcgcc tttttatcat ccccttctcg ccagcgttgc cccatcggca aaatacgctt
    40861 taatccccgc aagaatagac tccgcaactt cctgctggaa agtcgccgtt ttcagtttac
    40921 gctcttcctc aacgttactg ataaacgccg tttcgaccag aatggaggga atatctggtg
    40981 cctttagtac ggcaaacccg gcctgttcaa cttgattttt atgcagcttg ttgattttac
    41041 cgagcttatt cagcactgct ttaccaaact taaggctgtc agcaatggtc agcgactgca
    41101 ccatatcgaa catggtgtgg tcgacatagc ggtcaccgct tttgctcacg ccaccaatca
    41161 agtccgaggc gttctgggtt tgtgccagat attttgccgc agtactggtt gcacctttgg
    41221 ttgagagcgc aaacaccgag gaaccgctcg gctggcgact ggtaaaggcg tcggcatgga
    41281 tagagacaaa caggtcagca cgctgtttct gggcttttgc tacgcgcact tgcaacggaa
    41341 tgaagatgtc ttcattgcgc gtcatgtaca ccttcatatt gccctctttc tcgatcagag
    41401 agcgcaaacg gcgagctatt tgcaatacca catctttttc gcgcgttttg tatttcccca
    41461 ccgcaccgga gtcttcgcca ccgtggccag ggtcaagcat aatgacaatc ggacgatcgc
    41521 gccctgcttt acctggttgt ggaccacttt gcgctggcgg cacctgcttt tcgaggtcgc
    41581 ctttgttgta atcctccagc agcgccagca gcgggtcctg catatcctgt gcattggccg
    41641 gatagaggtc catcaccaga cgctctttaa acccggcgac cggcgcaagg gcaaacagct
    41701 gcggttttac gttttgcttt aattcaaaaa ccatccgtac ggtttgcggg tcaaattgcc
    41761 cgacgcgcgc cgacttgatg aacgggtcgt ctgcacgaat ttgtgccgcc atccccttga
    41821 gtaccgagtt caggtttaca tcttcgatat ccaccaccac gcgttcaggg ttactcaacg
    41881 cgaactgctt atatttcagc tgacgatttg attctaccgt cacgcgggtg tagctggacg
    41941 caggccagac gcgcaccgcc acgacctggc taaccgcagc caggctgacc tgacttacgc
    42001 tcaatagcca catagcgccc gcgccttgca gtaaacgacg acggctgatt gcagtgttgg
    42061 atcctgacat gcctctcccg agcaaaagaa atctaagctg tgtaacaagt aaacgactaa
    42121 tttgaccgga aactttagcg aaagacgcat aatctgtcat ctaataaacg gtaaacattc
    42181 tttttatatt cacggcatta ctgataaaaa agtcgctctc gcataaaatt tacacttgca
    42241 ccctgcgaaa aaacagaata aaaatacaat aatttcgaat aatcatgcaa aggggtgtgc
    42301 cgtggtaaag gaacgtaaaa ccgagctggt cgagggattc cgccattcgg ttccctatat
    42361 caatacccac cggggaaaaa cgtttgtcat catgctcggc ggtgaagcca ttgagcatga
    42421 gaatttctcc agtatcgtta atgatatcgg gttgttgcac agcctcggca tccgtctggt
    42481 ggtggtctat ggcgcacgtc cgcagatcga cgcaaatctg gctgcgcatc accacgaacc
    42541 gctgtatcac aagaatatac gtgtgaccga cgccaaaaca ctggaactgg tgaagcaggc
    42601 tgcgggaaca ttgcaactgg atattactgc tcgcctgtcg atgagtctca ataacacgcc
    42661 gctgcagggc gcgcatatca acgtcgtcag tggcaatttt attattgccc agccgctggg
    42721 cgtcgatgac ggcgtggatt actgccatag cgggcgtatc cggcggattg atgaagacgc
    42781 gatccgtcgt caactggaca gcggtgcaat agtgctaatg gggccggtcg ctgtttcagt
    42841 cactggcgag agctttaatc tgacctcgga agagattgcc actcaactgg ccatcaaact
    42901 gaaagctgag aagatgattg gtttttgctc ttcacagggc gtcactaatg acgacggtga
    42961 tattgtctca gaacttttcc ctaacgaagc gcaagcgcgg gtagaagccc aggaagagaa
    43021 aggcgattac aactccggta cggtgcgctt tttgcgtggc gcagtgaaag cctgccgcag
    43081 cggcgtgcgt cgctgtcatt taatcagtta tcaggaagat ggcgcgctgt tgcaagagtt
    43141 gttctcacgc gacggtatcg gtacgcagat tgtgatggaa agcgccgagc agattcgtcg
    43201 cgcaacaatc aacgatattg gcggtattct ggagttgatt cgcccgctgg agcagcaagg
    43261 tattctggtg cgccgttctc gcgagcagct ggagatggaa atcgacaaat tcaccattat
    43321 tcagctcgat aacacgacta ttgcctgcgc cgcgctctat ccgttcccgg aagagaagat
    43381 tggggaaatg gcctgtgtgg cagttcaccc ggattaccgc agttcatcac ggggcgaggt
    43441 tctgctggaa cgcattgccg ctcaggcgaa gcagagcggc ttaagcaaat tgtttgtgct
    43501 gaccacgcgc agtattcact ggttccagga acgtggattt accccggtgg atattgattt
    43561 actgcccgag agcaaaaagc agttgtacaa ctaccagcgt aaatccaaag tgttgatggc
    43621 ggatttaggg taagcgatga aaatcgccgg atgcggcagg cgtaacactc gtgcgtcgca
    43681 tccggcaatt acgtttatcc ccgcgaacta aacaacgccg ccagaccact gcgccgctca
    43741 gtacgagtgg cgattgccgc acttaatata cgctcatcgg catacagcga cagacggcga
    43801 cgcgcgcggg tcaccgcggt gtataccagc tctcgcgtta ctatcggcgt gcgttggctc
    43861 ggtaaaatca acgccgcatg gtcgaactcc gatccctgcg atttatgtac cgtcatcgcc
    43921 cacgtagttt cgtgctctgg caagcgactc ggttgcacag acttaatatt gccgtccggc
    43981 atcgcaaacc agacgcgcgt cccctgcccg cgatccagcg caataccgat atcgccatta
    44041 aacaacccaa gcgcactgtc attacgggca atcatcaccg gtcgaccttc gtaccaacga
    44101 gagtgcgaat gacgatgaat tttgcgcttc tgttgcataa actgctcaat tcgctcattc
    44161 agtccagcca cgccaaatgg cccttcccgc agggcgcaca aaagttggta ctcattgaac
    44221 gcctgaatga ttaaatccgg ctcggcacgc gcttgcagca gatccagata acgtccataa
    44281 cccgcaagag cttcctcaag catcgcaata taatcttcgc cgctctgtaa aagccgtttt
    44341 tcaatatcag taaaatcctg ctgaaaaacg gttttcactg ccgttttatc accacggttg
    44401 atcgccgcag ctaactgacc aatgccagaa tcgctgccga aacgatagct tttttgcagc
    44461 aggcagagac tgtcgcgcaa agatgccgct tctgtgccgg ttcctgccgg aacgtgagtt
    44521 cccgtcaggc ggcttagctg cccggcacgc tcggcggtaa agccggcatt ggcataagcg
    44581 cagatatcgc ccagcacagc cccagcctca accgaggcca gttgatcacg atcgccgaga
    44641 aagatcactc gcgcatgatc gggcaaggcg tcgatcagtc tcgacatcat aggcagatcg
    44701 atcattgacg cttcatctac caccagcaca tcaagatgca gcgggttacc ggcatgatga
    44761 cgtaaacgct ggctacccgg ctgcgcgccc agcaatcggt gcaaagtgct ggcatcttcc
    44821 ggaatgcgtt tcttttgttc atcggtcagc ggtaactgtc gcaaagcctt gccgagagat
    44881 tcggttaagc gcgcggcagc tttacccgtt ggtgcagcca gacggatacg gcagcgttcg
    44941 ccgtcggcca tttgaattaa cgctgccagc aacttcgcca cggtggtcgt tttaccggtg
    45001 cctgggccgc cggaaatcac cgagatccgc cgcgtcagcg ccactgctgc cgccactttt
    45061 tgccagttaa tttcatcgct tactggaaaa agtttgtcca gggtttgcgc cagtagagct
    45121 tcatcaacct caatggcatg attcacctca ttgaagaagc gcgctaccgt ccgctcgtta
    45181 caccacatgc gattcaaata aagacgatcg ccacagagga tcatcggcgt gggttcatct
    45241 cccctgctga ccgcttgaga agccagcaag cattcttccc aattttgtag ctcaccgatt
    45301 tcactgacac aggtcgccaa cagcggatgc gacgcctcgt tattttccag tcgtgaaagc
    45361 ggcaaacaga cgtgtccctc tccggcatca tgacttaaca gtgccgccgc gagggtgacg
    45421 gcaggatgtt catctcccgc cacggtcagg gcaaattgca catccagcgg gcgtagctgt
    45481 ttgtgctcca cagcttccag taattgcttt tgcaatttca ttacgcctcc tccagggtca
    45541 taccggcaaa catctcatcc atcagggcaa tcaacccggc gttgggtcgg gttgtgtaaa
    45601 tcccttgttg cggatgttct ttatcaacgc cacgcaggaa cagatagata acgccgccaa
    45661 agtgacgatc atagtcgtaa tcagcaatgc gatggcgcag ataacgatgc agcgccaggg
    45721 tataaagctg atattgcaga tcatagcggt gtgcctgcat cgctgctgcc atagcctgtt
    45781 gggtgtaagc cgaactgtct tcacccagcc agttggattt atagtcgagc aggtaataac
    45841 gcccttcgtg gcggaatacc aggtcgataa agccttttaa catgccgcgt acctgcatga
    45901 actccagcgg cgggcagcca gcggatagcg ggtcaaactg gcgaattagt gcatcaagct
    45961 ggctggcgat gagcggttcg ctaatcggca ggtaaaactc catctccacc tgtttatcgc
    46021 ggtcggaaag ctgactcagg ctaacacccg tttcattgag aggtgcctgg aggacagccg
    46081 tgatccactc ggtcaacacc ggttcccact gcgattcaaa gccgccgagt tccagttttt
    46141 cctgtaccca gttcgggtca accggctggg taaaatcgag gtcttcaaac aaactgtgca
    46201 agaatgtccc cggtgacgca ccgcgcggga actgatgcgg tgttaacgtc ggttcttcaa
    46261 cgacgctgac cacgcctgcg gcatcaacat ccagccgtgg catcagatcc tgagcgatac
    46321 cgtgaccacg ctgctgcaag ccggagtagc tggtgacacg ccagttatcg ccgggcaatc
    46381 gttgtagtgt cctcgcattc agttctgcag ttaaagcatc attaacctgc cagggctggt
    46441 tatcaccagt ttgtgccgtt tgccaggcaa tatcatcatc gcataacgct tcaatacagg
    46501 tgcgaagccc tgccgcatct tgcggttccc ctttttgcag caaacgcccg agcgcacttt
    46561 ggtggacgtc ggtgtcacct tttttatcgc cacgacggcg caccagcggt gcaacgccga
    46621 gactgcaatg ccaaaccgaa cgcgtcagcg ccacgtaaag caaacgcaga tcttccgcca
    46681 gacgttcgac ctccgcgagg tcgacgcttt ctggcgcagc attaagatcc aaaactgcct
    46741 caaacgagtg gcgatcgtga taaaacgcct gatcctggac gcggaaattg gtaataaatg
    46801 gcaaccagac cagcggatat tccagccctt tcgatttatg gatcgtgaca atctgcacca
    46861 gatgtttatc actttcgagg cgcatttgtt ggctggaggc attactgtct ggctcgagga
    46921 tatgttgcga taaccagcgt accagcgcat gttcactttc cagctgcgtt ccggcttctt
    46981 gaagcagttc gctgatatgc aagatatcgg taagacgccg ctcaccgcct gccgttgcca
    47041 gcaagttttc tgcaatgtta cgcgccgaca tcagcgcccg cagcatcggc ataacgccac
    47101 gtttgcgcca gatttgccga taaccatcga actcttcgac taccgcatcc cacgcatgtt
    47161 cgtcattatt cagcgtttca atatccagcg cattcagccc catcattgac gttgccagcg
    47221 cactgcgcag ggtgttctca cgttcgggcg tcatcaccgc ctgcaacagc caaagcattt
    47281 cctgcgcttc cagagtttca aaaacactat cgcggttcga aaggtaaacg gaagggattt
    47341 ccagcaacgt taaggcatcg cgcacctggg cggcctcctg gcggctgcgc accagcacgc
    47401 tgatgtccga agcacgcacc ggacgcgcgt cgtcgccgtt catcagcaac gcttcgcccc
    47461 gctgtccggc ttgtagccag tcgcggattt gcgcagcaca tacctgcgcc atggtacttt
    47521 gatagtcgcc aacgccgcag ctttcgcctt ccatcagcca cattttcatc gcaggctgcg
    47581 tttcaccttt aaagacaaaa cgtaacgcct gatttttccc ggctgatttc actggaataa
    47641 acggtatttc gcgaaacatg aacgcgtcat cagtctggct gaaaagcttg ttcacactgt
    47701 tcaccattcc aggtgcggag cgccagttgg tgtctaaagt gtagtgggcg tgaacttcac
    47761 tacgcgcctt catataagtg aagatatccg caccccggaa tgcatatatg gcctgcttcg
    47821 ggtcgccaat cagcaacaat gcggtttctg gctgatggtg ccagatacgg cgaaaaattc
    47881 ggtactgctg ggggtcggta tcctgaaatt catcgatcat tgccaccggg aatcgcgtac
    47941 ggatcgccgc tgccaacacc tcgccgcttt cgctacgcag cgcggaatcg agccgactta
    48001 acatgtcatc aaaacccaat tcgccacggc ggcgtttttc acgcgctact gtttcgcgga
    48061 tctcagccaa tgcgcgggtg ataaccagat cacggatcga caatggttct gcaagcagtt
    48121 gatcgatcgc ctcaaacagt ggatgtcgcg gggtttcccc cccggctttc gtgcgatctt
    48181 ctaagaaacg ctgggagaat ttttccagcg actccggcaa ctgataactg tttgtctctt
    48241 cttctgccca ggcgctgatc ttgtcgatcc atttagcctg attgctacgg ttaaacttgc
    48301 gtcgatcaat accagaagat tcgatcagcg catccagttc acccactgcg tcgcgccact
    48361 gctgttttac cgtatcaata cgcgccacaa tttgcgcgtg acgggaagct agcgtttcat
    48421 catcgggcgg cggtgctttg ataaccggcg cttcgccttg cagataacga ttaatatcgc
    48481 gcagcaacgc ctgcggccct ttccaggttt caaagacgac ctgggctatt tcacgcggca
    48541 gcgggtagca gtggcgacgc cagaaatcgg cgcaggcctg gtagcgtagc agagactcat
    48601 cttcaatcag ctgctgctca aacagcatgc cggattcaaa ggcattcagg ttgagcatgc
    48661 gctggcaaaa gccgtgaata gtaaagactg ccgcttcatc catctgccgt tcggctaaca
    48721 acaaccactg cgcggcttgc gctttatcgt cgatctcttc cagcaggtgt tcgtacaatg
    48781 gattgtcggt ggtttcacgc agacaggcga tgcgcaactc gtggatattg ctacggatac
    48841 gaccgcgcaa ttctgccgtg gcagcctcgg taaaggtgac caccagcagt tcttcaacgg
    48901 tcagcgggcg gggaaaggcg gcggaaccgc ctagtccaag taacaggcgc aaataaagcg
    48961 ccgcaatcgt aaaggttttg cctgtgccgg cagaggcttc aatcaggcgc tcgccctgta
    49021 agggcaagcg caaaggatct agtgtctcgg cgacatcact cattcttttc actcatcagg
    49081 ggcattgttt gctgcaacgc gctgacgttc tcccacactt tccagccttc aggatgcaca
    49141 tattcggctt tcccgttttg gctgccggaa atctgcgaca ggatagccat gccttgcggc
    49201 tcgactaccg cctgatggaa gaaatcggcc agtttttgcg gcgtcagcag tttaatctgg
    49261 gccacgattt tatcacgcga atcgaagcgc atattgccgc gatcgaaatc tttacttaac
    49321 ttcgatttcg ccgagcgttt gcggtgcctg cagcatctgg gtaattaccg cctgctggat
    49381 ttgcgcaaac tcatctggct tcatcgctcg caattttgcc tctgcggttg ggaaaaacgc
    49441 cttgtaacgc tcccacaaga atgaaggctg tttatcattg ctttgcaaaa ggaagcccat
    49501 gccccactga cgccccacgc tcattggaaa cgcaaacacg gcatagccca attgttcttc
    49561 ggtacgcaac tgattgtaga accacggctg tacgatctgc cccaacagag agctataggc
    49621 tgagctggtg tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggt
    49681 gctgttaccg gctttttcaa agatgacgga ttgtttttta tcgaccacta catctttgtt
    49741 tcgacaccac tctgaaccat cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt
    49801 tgcctgggcc tcggtcatgt tgccgataac cataaactct ggtcgagccc ctgattttaa
    49861 ggcgtcgcga taagccagca cctctttcaa cgtaatggag ggcaaaattt tacgccgttc
    49921 atctcgcgag aagtacggca cttgcgagag catctgcgcg ggcataatcg cctgctcaaa
    49981 cgctttaccc ttttctgcgg aatccatcat ctggttatac caggacttcg cctgctcaag
    50041 ctgatcttcc gtagcggtat agctaaagta cccctcgagc aatgcctgga acagctgcgg
    50101 cagacgctgg gggtaaccat tagcattaac cataaggccg ttgttagcgt tggtggaaaa
    50161 acttatgcca ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata
    50221 atcattgagc gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa
    50281 aatcaggctg acatcagctt tgggttcgct ggaaaaataa cggcttggcg catacaccac
    50341 gcgcagattc gactcatcaa caatcagctc tggatggtca tatttcttct ctgacttaat
    50401 cagcgagaaa tcgtcaggaa tataggggtt aagctccggt aaggagagcg caatattggc
    50461 agctttttgc tgccagtcag cgaaagtttg ttcgctgatt ttatcgacct gatacggcgc
    50521 atcgacaaaa taagccgttt tattgtgcgg ctctttcggg ctgatatacc agatacgcgc
    50581 attctgcggc gtcatcatcg ccagacgttc ttttactgct ttagcatcgt accgatcggc
    50641 aatattgact gcatccagcg tatgctcaac cggaacgcga atcatggtat ctgccagcca
    50701 ttcgacgtaa tccatatcac gggtgatcga cggataacgg aagtcgatat ccagcacatt
    50761 cgccagttca tcgaagtatt gtttatcaat gcctttttca cgtaacagat tgagatagct
    50821 aaaaattgcc gccacaacct gatcgcgatt agccaggcct ttatcggtta aagacgcaga
    50881 gatcgctaat acgccgctgt tgccgttgac gataggatcg gagttggcgc taatgccctc
    50941 aactaatccc tgcttttgca gccagtcgga aagtgtacct gggctgcgat taccaatcag
    51001 ataggtaatc aattcatccg ttttactacg gaacttcgct gagttgttat cgatgcgaaa
    51061 ctcaacgcgc agaactttac gcggcattgc cgggacgtaa tgaatgataa tgcccttttg
    51121 cgcgtcggtg actaccggca cggtgatttc cggttttttg ctctctttgt tcggcacgcg
    51181 accaaaggtg tccgccgcca tttttgccaa ctccggcagc ggtttattgc tgtaaataac
    51241 cgccttcatc agattggcgg aatagtactt ctcgtggaaa tctttcagcg cctgctgcac
    51301 cggattacca ggtttatcgc ttaaagtttc gaggttacca ccagaaaact ttgaaccggg
    51361 gtgtgccggg ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg
    51421 cgccatggtt aattcagcgt tcaccgcatt acgctcacgt tcggcatatt tcttgtcgag
    51481 caaaggttca gcaatagcat cggccaggcg gtctaccgca ccaggcaagg cgtcgttctc
    51541 aacttccaga tagaaagccg tgcgatacgg cgcagtgctg gcattgtgac taccgccgtg
    51601 cattttgaga tattcggcca gactgtcagc ctgcgggtac tttttcgacc ccatcagact
    51661 catatgttca aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca gcgacccaac
    51721 gggcaccacc agcgccgaga gcgatttaac tgcctgcgga tcagaaacca gcaagaccac
    51781 cataccgtta tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat
    51841 ggtttcctga atcggctgcc atcccgtttc tgcctgactt aagggtgccc aaagggcaac
    51901 taacaacaat aatgctttga accaggtgct gcggggcatt ctcggacctc ataatcaact
    51961 taattttctg tccagattca acacgttaac gcaaatcatc tgccagaatt taatcttgtg
    52021 ctgcacgagt cagcctatgt ttatataacc atcagtccgt gactggtgcg catcataaag
    52081 taagcggata gattgcgcaa tttttataca gcactcatga ctgattaaag cgaaacagcg
    52141 gtaacaggaa acgttgcgac tgttcaacga tggcctccat tgtctctggt gttaattgcc
    52201 gccagagcct ttgataccag atatcatcac cttcgccacg caccatcatg ttgccttcgt
    52261 aagcctgaag gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc agcatggcat
    52321 cgttttgcgc gtcataacag gtttttagcc acgcgccgcc actttcaggt aacaccagca
    52381 atggcgcgga catcccttca cgatacccct caatcagttg tgagaggtaa tgcaaagcct
    52441 gttcggctgc aagcggcgga aaacgccact cgccgtcttt gcgtagaaaa aggcgacttt
    52501 caccattacc accgctggca cagtagacaa ggtgttccag ccaaagttgc attccctgcg
    52561 ccacacttaa taaagaggga cgccagcgca acaggccatc cggctgcacc tgcggcaacc
    52621 agccagttat ctgcacaccg ttgcaggcga gatcaatttc catactctgc cccggctggc
    52681 gacaggcaat gactctgtcg gcaagctgct gcatctcctg gcactgagtt tcccagaaaa
    52741 tttcaccaaa agcgccatac ggtaaatccc ctgccgcccg gaagcgacgg aacaagcgtt
    52801 cggcatcatc ctgctcaacc agtgcattca ataactgctg attgatttga taacggctaa
    52861 gcccttccag aataaatggc tcggtgtcgg ggatttcgct gtcttcagta cggaagttca
    52921 cctgcaaacg catctggaag aacgcccgca ccggatgtgc ccagaatcgt tgtagcgttt
    52981 ccagcggcac ggtttccggt aaggtaaacg gcagcggctg aacaaattca gaatgtgctt
    53041 taccagcctg gctggccgca ggtagccatt cacgagcata gctttgtcgt tcgcctggct
    53101 gatagttttg tggatcaaac ggcattcggg tatggaggca agtaagatgc gcttttaccc
    53161 ttgcctcgct ttcatcacag ttgagcgctt catcgcccgg tagataatga ctttgcccga
    53221 tgtagtcgat cagttcctgc accagtaccg acgggaaacg ttcactgtta tcctgaatgg
    53281 aacgcccgat atagctgata tagagttttt gctgcgcgga aattaacgct tccaggaaca
    53341 gatagcggtc gtcatcgcga cggctacggt cgccacgctt cggtttctgg ctcatcaggt
    53401 caaagcccaa tggcgcaagc tgacgtggat aaacgccgtc gttcattccc agcaggcaaa
    53461 ccactttgaa cggaattgaa cgcattggca tcagagtaca aatgttaacc ggtccggcga
    53521 gaaaacgctg gctgatacgt tcttgatcca gacgctgtgc cagttcatca cgcaatagtg
    53581 acagcggcac cgcgtcgcca tactgcgcac ctaaaccttc ggcgataatc gcctgccatt
    53641 gttgttcgat cagcgtcatc gccgcttcgg tttccgcatc cggcaggaag aaggcgctga
    53701 gcatatcgcg acaaaccggc aaccactctt ctagcggacg ctcctgtgcc agcccgcgac
    53761 gccagatgtt tagctgcatt agcagtgaag ccagatgccc caccagttct gcaattaagc
    53821 cgctcgattc atcataaggt agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt
    53881 agcccagcaa catacgcgtc aggccaaatc gccaggtgtg ttgtccggtg gcggggagtt
    53941 ccagctcgcg aacgttgtcg tcatctatcc cccaacgaat gccggattcg ttgacccact
    54001 ggcgtaaata acgcagccct tcttcggtga tgtcaaaccg cgccgccagc accggcacat
    54061 ccagtaatgc cagcacgtct tccgacacaa agcggctgtc tggcaacgat aacaggctga
    54121 taaacgcttc cagtaccgga tgcgactgcc gcgcccgacg gtcggaaatg gcgtaaggca
    54181 ggtaacgatc cgcaggtgcg ctaccaaaca cagcccgaat aaacggactg tagctgtcga
    54241 tatcagccac catcacgatg atgtcgcgcg gagtaagtgt cgggtcttct tccagcatcg
    54301 ccagcaggcg atcgtgcaaa acttcaactt cacgctgcgg gctatggcaa acgtggaagg
    54361 tgatactgct atccagtgga tcaagcgggc gtttgttatc gctacgggaa aactcttcga
    54421 tgttcacacc agcaacggca cggttttcca gttccagaat gtcagactga atgttatgca
    54481 gcaggttatc tggcgtgaca tcgacaaagg cgtccagctc ctggctgctc tccaggtcag
    54541 aaaggagata aatgtagtcg cgcccaagct taccccatga agccagcagc gggttgccga
    54601 catcctgttc accatcgctg ttaaagagct gcccggcatt ttcgctatca cgaaatagcg
    54661 gcaattcgcg atcttcaaaa ctgtgtcgac gctgacgggt cagcagtttc gccagataag
    54721 caggatcttt aatatcgccc cagtaataac ggcaggggtt ggtaaacagg agatggattt
    54781 caatatgttt acccagcgcc tgtagcgcct ggagataaac aggcggtaac gcggaaatac
    54841 cgcatataaa gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt
    54901 cgataaagcg ctgatagaga ttggcgcggt gccagcgcgg ttgcccgagt tgatgggtat
    54961 attccaccag cgccttccac aacggggctt gccaggcctg tgcttctccc agcccttcaa
    55021 ccaaatgtcc tgtttcccac tgtgccagcc agtccggacg atagaccaga tactggtcaa
    55081 acaggtccgc cgcttttgag gaaagctgga acagttttcg cttgtcgcta tcgtcagtca
    55141 gataatgccg caacagggta aagtcttcgc gctccagcaa ttgcggcagc agagtcatca
    55201 gtttccagct catgctctgt ttgttaaagg cgctctcttt ggggatttcc ggtaacaccc
    55261 ggacgaacat atcccagata aagctcgctg gcagcggaaa atcaatgttt gccgcaatac
    55321 taaacttttg cgacagggtc atttgcagcc actgcgccat accggtactt tgcaccagaa
    55381 tcatctctgg ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac tccatcaacg
    55441 cttccagcac gtccagacga ttggaatggt agacccttaa catagcggct cctgactact
    55501 gacgattcgg gcaatgcagg cgggtcatct cgccttctct gcccccgggt gaaactagcg
    55561 taacgctgat gctgacacat cccgcctgcg atgtctgcat tcggttgacc tgccagttgg
    55621 caggtggcga aatcgcgcgc agttgcgttt gctgccaacc atgccgccag agctgttggt
    55681 actggtttct gctggcgaga ctgttcatta atgttcgctg gatacccgat aacgcagtga
    55741 caatcatcac catcagcacc atcgcaacca ttacctccgg caggctaaag ccttgttgat
    55801 tcttcaggga agctgacata acgccctctc tttcagcgga caaaaatcgc tccagccgcg
    55861 tggcgaaaaa acaatgtttc catcaatgac ttcgcctgtt cgccacaacg aaacgccttc
    55921 ataaccggca accaataagg cttcattatc tgccagtaaa cgcaaacaaa cccgggcatc
    55981 ggttccagcg tactgcgagc actgaactgc gggctgcgtc tgccaggagt gcattttccc
    56041 ccacgccagc gccgactgaa cgatggcctg gcggcgcaat gactgacttt ctatgctcac
    56101 gcgagaggca aaactgcgat cctgctgact cattccttgc aatagcaagc tacccaaaac
    56161 cagcagcatc aggaccagag ccagtgacga aacacctttt tcgcggttca caggttgaat
    56221 cctgtcacgc tatagctggc atccaccacg gtttgcggtt cagacttact ggcagcacgc
    56281 atattaaccg tcaacaccgg cgagaagccg ctgacatcct gacgtacgac ctgaaaagtg
    56341 tcgataatga tggcatccgg attagtgact ttatcccagc ccttaccttc acaggatgtc
    56401 gcaccgcgta gcgtttccag cacatgctcc ttcagacgaa atccaatctg gtcagactct
    56461 tttaccggtt cgcgatccca gataccgtta ctgttcgcat cccactgcac aatgatacag
    56521 tcaccctgtc cgacaatttc cagcccttcg ccggtacagc tgccatgaca ataacccgcc
    56581 ctctggagat gcttcgcgac ggtaaatacc cgcagccaga tttcatcttc cagcgccagc
    56641 ttacgggtgc tcgttaaact ttcacgctgt aacgcaggca gaaagcgtgc agcccccagc
    56701 aacaatacgc tactgatcgc catagcaatc aacacttcca gcagagaaaa accttgctct
    56761 tttacaggca tccttctgtt tctccttgct gacaaagccg gagtcttccc cacggcgaaa
    56821 ccaccagcca ccactcgccc gttgagtttt tgaagcgaat atgcccggcc catgcggtat
    56881 tgcgcaggcc aaagaaagca agcgaaggtg tcaggtcgct catttagact tcgggccagc
    56941 gtggcacaaa gaccaatggt gaactgccat gacaggtatt ggccccagca gcggaactca
    57001 caaggcacca taacgtcccc tccctgataa cgctgatact gtggtcgcgg ttatgccagt
    57061 tggcatcttc acgtaaatag agcaaatagt cccgcgcctg gctggcggtt tgccatagcc
    57121 gttgcgactg ctgccagtat tgccagccat agagtccact tgcgcttagc atgaccaaaa
    57181 tcagcatcgc gaccagcgtt tcaatcagcg tataaccacg ttgtgttttc atgccggcag
    57241 tatggagcga ggagaaaaaa agacgagggc cagtttctat ttcttcggcg catcttccgg
    57301 actatttacg ccgttgcagg acgttgcaaa atttcgggaa ggcgtctcga agaatttaac
    57361 ggagggtaaa aaaaaccgac gcacactggc gtcggctctg gcaggatgtt tcgtaattag
    57421 atagccaccg gcgctttaat gcccggatgt ggatcgtagc cttcaatctc aaagtcttcg
    57481 aaacggtagt cgaagatgga ttcgggttta cgtttgataa tcaacttcgg cagcggacgc
    57541 ggttcgcggc ttaattgcag atgagtttga tccatatgat tgctgtacag gtgcgtatcg
    57601 ccaccggtcc agacaaaatc acccacttcc agatcgcact gctgcgccat catatgcacc
    57661 agtaacgcgt agctggcaat gttgaacggc aggccgagga agacgtcaca ggagcgctga
    57721 taaagctggc aagagagttt gccgtctgcc acatagaact ggaagaatgc atggcacggt
    57781 gccagcgcca ttttatccag ttcgcctacg ttccacgctg aaacaataat gcggcgggaa
    57841 tccgggtcgt ttttcagctg gttcagtacc gtagtgatct ggtcaatatg acgaccatct
    57901 ggcgttggcc aggcgcgcca ctgtttacca tacactggtc cgaggtcgcc gttttcatcg
    57961 gcccattcgt cccagatggt gacattgttt tcgtgtagat aagcaatgtt agtgtcgccc
    58021 tgcagaaacc acagcagttc gtggatgatg gaacgcaggt ggcaacgttt agttgtcacc
    58081 agcgggaatc catcctgcag gttaaaacgc atctgatgac caaaaatgga aagcgttccg
    58141 gttccggtac ggtcgttttt ctgtgtgcct tcgtcgagca ctttttgcat cagttctaaa
    58201 tactgtttca tggttcctca ggaaacgtgt tgctgtgggc tgcgacgata tgcccagacc
    58261 atcatgatca cacccgcgac aatcatcggg atggaaagaa tttgccccat gctgatgtac
    58321 tgcacccagg caccggtaaa ctgcgcgtcg ggctggcgga aaaactcaac aatgatgcga
    58381 aacgcgccgt agccaatcag gaacaagcca gagacagctc ccattgggcg cggtttacga
    58441 atatacaggt tgaggataat aaacagcacc acaccttcca gcagcagctc gtaaagctgt
    58501 gatgggtggc gcggcagcac accgtaagtg tcgaaaatgg attgccactg cgggttggtt
    58561 tgcagcagca aaatatcttc tgtacgggag cctgggaaca gcatggcaaa cgggaagttc
    58621 gggtcaacgc ggccccacaa ttcaccgtta ataaagttgc ccagacgccc ggcaccaaga
    58681 ccaaacggaa tgagtggtgc gataaaatca gaaacctgga agaaggaacg tttagtacgg
    58741 cgggcaaaga taatcatcac cacgataacg ccaatcaggc cgccgtggaa agacatgccg
    58801 ccgtcccaga cacggaacag atacagcgga tcggccataa actgcgggaa attgtagaac
    58861 agaacataac caatacgtcc cccgaggaag acgccgagga agcccgcata gagtaagttt
    58921 tcaacttcat ttttggtcca gccgctgccc ggacgattcg cccgtcgtgt tgccagccac
    58981 attgcaaaaa tgaaacccac cagatacatc aggccgtacc agtgaagcgc cacgggtcct
    59041 attgagaaaa tgaccggatc aaactccgga aaatgcagat agctactggt catctgtcac
    59101 cacaagttct tgttatttcg ctgaaagaga acagcgattg aaatgcgcgc cgcaggtttc
    59161 aggcgctcca aaggtgcgaa taatagcaca aggggacctg gctggttgcc ggataccgtt
    59221 aaaagatatg tatatgatcc gcgctataac cctccgcgaa tcagcccgcc catgccgcga
    59281 cgctccataa aggctgcaac ctgatggcga acttcggtcg ccagttgcgc ttccagacta
    59341 cgctgcgcaa gattttctgc ttcggcataa tcaatgcgcc gcagcaggta ttttacccgc
    59401 gctacagaac gtccgttcat agacagatgg cgatacccaa gcccaatgag gattgccacg
    59461 cacatgggat cgcccgccat ttcaccgcac aaacggagat cgattccatg tatttccgct
    59521 tcccgggcga tcatcgccag agctcgtaac attgcaggat gaagactgtc ataaatgttc
    59581 gccacccggg tattgttgcg atcaacggcc agaatgtatt gagtcagatc gttggtgcca
    59641 acagagatga aatcgacccg ctttgccaga tgcggcagca taaataccat tgacggcact
    59701 tccagcatga tgccgatacg tggtttggga atttcgtaac cgatcatctc ctcgacttca
    59761 cgtccggcac gttcaatcag gcggcgtgct tcatcaactt catcgaggct tgtgaccatc
    59821 ggcaacagaa tattcaggtt gcccgtagcg gcattagcac gcagcatcgc ccgcacctgg
    59881 atcaagaaga tctccggctg atcgagcgta atgcgaatcc cacgccaacc caggcatgga
    59941 ttctcttcgc tgatcggcat gtaaggcagc tgcttatctg ctccgacatc cagcgtacgc
    60001 aaggtgacgg gtttatcatt gaacatttgc agcatcccct gatactgcgc cacctgttct
    60061 tcttccgacg ggaaaccact ttgcagcatg aatgggattt cagtgcgata aagaccgatg
    60121 ccatcaatac ggctgcccag tttttcttca tgttccgggc ttaaaccagc attgagcatg
    60181 acttttatac gctcaccgct ttttaactgc gcgggtaaat tgacgtcatc ttccgccaga
    60241 cggctaagct caatctcttc actaattagc cgctgatatt cttgcagcag taccggctcc
    60301 ggatcgacca gcaattcacc gcgatagcca tcaacgatca gcgtcctacg atgcagcacc
    60361 gaaggctgaa tatccgcgcc catcacggta gggatcccca gcgcacgtac catgatcgca
    60421 gcatgggagt tggctgcgcc atctcgcacg acaacaccga ctaagcgatc ctggggcagc
    60481 tcagcaagag ttgtcgctga cagttcatct gccaccagaa tgaaacgttc cggccaggcg
    60541 ttcggccctt gattagcgtc atcaagatga aacagcaatc gctgacccag cgcacgtaaa
    60601 tcgccagccc gctctttgag atagttatcg cttagcgcgg caaactgttc ggcaaatttt
    60661 tcaatgaccg tttttaccgc ccactctgcc accgagcctt tatcaacctc ggcaaacaat
    60721 tcgcgacgca gccgggtgtc cgaaagtagg tgcgagtaaa gatcgaaaat agccgccgtt
    60781 tctttttgtg ctccggcggc aaagcgtttg ctgtagcggc gaaactcgtt tgccgcttct
    60841 tccagcgccc cggtcagtcg ttcgcgttcc agagccggat ccagcgttga tgcctgatac
    60901 acctgttcca ttaaaggtaa cgtggcatcc tgccagcctt cggcaatcgc cacaccaggt
    60961 gctgccggta atgcgcggat tcgcgtctgg cgatattgcc caaacaaggc agtcaactgc
    61021 gactgagaaa gaatagctgc catctgggtg gcaagcgtca ccaggaagga ttcttcactt
    61081 tcgtcatact ggcgcaactc tcgttgctgt accaccagta caccaagcaa ctggcgacgt
    61141 tgaataattg gtacgcctaa aaacgcgcgg aaacgttctt cttttacgga ggggatgtat
    61201 ttgaagctgg ggtgcttttg cgcatctgca aggtttatcg gttccgccag cctgccaacc
    61261 aggccgacga tcccttcatc aaacgcgagc gttacagtgc gaccgcgtgg ttttttcagc
    61321 ccccgggtcg ccatcaggta gtaacaacgt cgatcatgat cggccaggta gaccgaacag
    61381 acctcggtat ccatcgcaag acagatgtcg gtaaccagaa tatttaacgc ctcattcagg
    61441 cgtggtgcgc tggctacctt ttcgactatt tcgcgcaggc gagtgagcat aattggcgtg
    61501 acttaacctc ttttacgtcg ataagcagat gtgttttgtg gttttggcgt attttcctgc
    61561 agtgacatca ccacactcgc gaactctttc attaccctac ggtagacatc acgtttaaat
    61621 gacaccacct gtctgaccgg ataccagtaa cttacccatc gccagccgtc aaactctggt
    61681 gtactgctgg tttgcatatt gatttctgca tcgccgctca ccagctgcaa gagaaaccat
    61741 ttttgttttt ggccgataca aaccggcttc gtgtcccaac gcaccaaacg tttcggtaat
    61801 ttgtagcgca accagttacg cgttgaagca aggattcgaa cgtctttgcg gcttaatcct
    61861 acttcttcaa acaattcacg gtacatcgcc tgctctgcgg attctccggg gttgattccg
    61921 ccttgcggaa attgccagga gtgctgacca aatcgccggg cccacattac ctgcccctgg
    61981 cgattacaaa tcacgatacc tacgtttggg cggtagccat cgtcatcaat caccggacta
    62041 cctcaaaata aagctttata tacgaatgat tgtttcatac tccaagaaga cggtaaacca
    62101 ctctctgcag ggcattacac actaataaca attgaataac tcacagttat gtgcagagtt
    62161 ataaacagag gaaggggtgg acagccgttt ttattcacct tttctgtgga tagagttgtg
    62221 aagaactacg gaattactac gggaaaaccc ggagcattct gaataagccc gatatagaca
    62281 ttttaaaata ttcattatta gtcaatgcat tacatgtaat ttgagtaatg aaaaagagta
    62341 attcgtgacc caggtcacac ctctcattta cgggttggcg aaagatcaac caatgccgta
    62401 tttatccaca gaatgtgcca ctaagttaag cactgaacca ctaaaaactg gagtttcgtc
    62461 gcacgtcaag gctgtaaatg gaaacagtag tggaggtttt tcacagttat cccagctttc
    62521 tgtggataac atggtgtaag atcctgttta ttttcagtga ccagatttgg aaaacctatt
    62581 gcagtgttgc gcaactcgat taccggcagc ctaaaaagca atataaatca gagaattgaa
    62641 caacacatgt ggaaaaagtt acactgcgaa tattcggcac ataattgctg tttgtttttt
    62701 aaccaaggta tcatgacatg tcccaacctc gcccactcct ctctcctccc gaaactgaag
    62761 aacagttgtt agcgcaagca cagcaacttt ctggttatac attgggagaa ctggcggcac
    62821 ttgccgggct ggttacgcca gagaatttaa aacgcgataa gggctggatt ggcgtgttac
    62881 tggagatctg gctaggtgcc agcgcaggga gtaaacctga gcaagatttt gctgctctgg
    62941 gcgtggaact taaaactatc cctgtggata gtcttggtcg tccgctggaa acaacattcg
    63001 tttgtgttgc cccgttaacg ggtaatagcg gggtgacctg ggaaaccagc catgtgcgcc
    63061 acaagctcaa acgcgtactg tggataccgg ttgaaggtga gcgcagcatc ccgctggcgc
    63121 agcgtcgcgt aggatcaccg ttgctgtgga gcccgaatga agaggaagac cgacagctac
    63181 gcgaagactg ggaagaatta atggatatga ttgttctcgg tcaggttgag cggatcaccg
    63241 ctcgtcacgg ggagtattta cagatacgac cgaaagcagc gaatgcgaaa gcgcttaccg
    63301 aagccattgg tgcccggggc gaacggattc tgacgctgcc acgcggcttt tatttgaaga
    63361 agaatttcac cagtgcacta ctggcccgtc attttctgat ccagtagcca tcgctttgac
    63421 ctgccgcttt ccgggcatat aattaccgct tcattttttt ggcagggctt tttagatgtt
    63481 atttgcatgg ataaccgatc ctaacgcctg gcttgcgctc ggtacgctga cgctgctgga
    63541 gatcgttctt gggatcgaca atattatttt cctttctctg gtggtggcaa agctccccac
    63601 agcacaacgt gctcatgcgc gccgtctggg gttggcggga gccatggtta tgcgtctggc
    63661 gctgctggca tcaatcgcct gggttacgcg cctgacaaat ccgcttttta caatattcag
    63721 tcaggaaatt tccgcccgtg atttgattct gcttctgggt ggcttgttcc ttatctggaa
    63781 agccagcaag gaaatccacg aatccattga aggtgaagaa gaagggctga aaacacgcgt
    63841 ttcatcattc ctcggcgcta tcgtgcagat tatgctgctg gatattatct tcagcctcga
    63901 ctcggtgatt accgctgtgg gtctgtcaga tcacctgttt attatgatgg ccgccgtggt
    63961 gattgccgta ggcgtgatga tgttcgccgc gcgctcgatt ggtgattttg tcgaacgcca
    64021 tccttcggta aaaatgctgg cgctctcttt cctgattctg gtcggcttta ccctgattct
    64081 ggaaagtttc gacatccacg taccgaaagg ttacatctac ttcgcgatgt tcttctctat
    64141 tgcggttgaa agcctcaacc tgattcgcaa caaaaagaac ccgctctgat actccgtacg
    64201 ctctcctgcg ggagtgcgta ctcactcctg ttaccctcac agtttaagat tttcctgctt
    64261 tcaagatata taacgtcggt ttataaacag actattatca taggtgggcc aaatgaggat
    64321 aaccggatga aaaaatgggc agtaataatt tctgcagtcg gactggcgtt tgctgtttcc
    64381 gggtgttcca gtgattacgt catggcgacc aaagatggcc gtatgatttt gaccgatgga
    64441 aaacctgaaa ttgatgatga taccgggctg gtgagttatc acgatcagca aggtaacgcg
    64501 atgcaaatta accgtgatga tgtttcgcaa attattgaac gttaacaaat aaggtcagca
    64561 tccggctgta atgccgatca gttaag
//
LOCUS       DAZPLI010000005        60957 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZPLI010000005 DAZPLI010000000
VERSION     DAZPLI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.005"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..468
                     /locus_tag="ACHUU3_000274"
     CDS             <1..468
                     /locus_tag="ACHUU3_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2080453.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="ACHUU3_000275"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="ACHUU3_000275"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR2080454.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="ACHUU3_000276"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="ACHUU3_000276"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR2080455.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHUU3_000277"
     CDS             complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHUU3_000277"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR2080456.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="ACHUU3_000278"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="ACHUU3_000278"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR2080457.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4084..5313)
                     /locus_tag="ACHUU3_000279"
     CDS             complement(4084..5313)
                     /locus_tag="ACHUU3_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR2080458.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5377..5817)
                     /locus_tag="ACHUU3_000280"
     CDS             complement(5377..5817)
                     /locus_tag="ACHUU3_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR2080459.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5922..6692)
                     /locus_tag="ACHUU3_000281"
     CDS             complement(5922..6692)
                     /locus_tag="ACHUU3_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR2080460.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6689..7615)
                     /locus_tag="ACHUU3_000282"
     CDS             complement(6689..7615)
                     /locus_tag="ACHUU3_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR2080461.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7724..8386
                     /gene="can"
                     /locus_tag="ACHUU3_000283"
     CDS             7724..8386
                     /gene="can"
                     /locus_tag="ACHUU3_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR2080462.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHUU3_000284"
     CDS             complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHUU3_000284"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR2080463.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9169..11559
                     /gene="gcd"
                     /locus_tag="ACHUU3_000285"
     CDS             9169..11559
                     /gene="gcd"
                     /locus_tag="ACHUU3_000285"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR2080464.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHUU3_000286"
     CDS             complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHUU3_000286"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR2080465.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEIPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13322..13669
                     /locus_tag="ACHUU3_000287"
     CDS             13322..13669
                     /locus_tag="ACHUU3_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR2080466.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13775..14641
                     /gene="speE"
                     /locus_tag="ACHUU3_000288"
     CDS             13775..14641
                     /gene="speE"
                     /locus_tag="ACHUU3_000288"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR2080467.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14657..15451
                     /gene="speD"
                     /locus_tag="ACHUU3_000289"
     CDS             14657..15451
                     /gene="speD"
                     /locus_tag="ACHUU3_000289"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR2080468.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHUU3_000290"
     CDS             complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHUU3_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR2080469.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHUU3_000291"
     CDS             complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHUU3_000291"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR2080470.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18978..20735
                     /locus_tag="ACHUU3_000292"
     CDS             18978..20735
                     /locus_tag="ACHUU3_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFR2080471.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="ACHUU3_000293"
     CDS             complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="ACHUU3_000293"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR2080472.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="ACHUU3_000294"
     CDS             complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="ACHUU3_000294"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR2080473.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="ACHUU3_000295"
     CDS             complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="ACHUU3_000295"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR2080474.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="ACHUU3_000296"
     CDS             complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="ACHUU3_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR2080475.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28591..29964
                     /gene="aroP"
                     /locus_tag="ACHUU3_000297"
     CDS             28591..29964
                     /gene="aroP"
                     /locus_tag="ACHUU3_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR2080476.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="ACHUU3_000298"
     CDS             complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="ACHUU3_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR2080477.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="ACHUU3_000299"
     CDS             complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="ACHUU3_000299"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR2080478.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31497..32390
                     /gene="nadC"
                     /locus_tag="ACHUU3_000300"
     CDS             31497..32390
                     /gene="nadC"
                     /locus_tag="ACHUU3_000300"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR2080479.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32593..33033
                     /gene="ppdD"
                     /locus_tag="ACHUU3_000301"
     CDS             32593..33033
                     /gene="ppdD"
                     /locus_tag="ACHUU3_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR2080480.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            33043..34428
                     /gene="gspE"
                     /locus_tag="ACHUU3_000302"
     CDS             33043..34428
                     /gene="gspE"
                     /locus_tag="ACHUU3_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFR2080481.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVNQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            34418..35620
                     /gene="hofC"
                     /locus_tag="ACHUU3_000303"
     CDS             34418..35620
                     /gene="hofC"
                     /locus_tag="ACHUU3_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFR2080482.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPTLLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35655..36698)
                     /gene="guaC"
                     /locus_tag="ACHUU3_000304"
     CDS             complement(35655..36698)
                     /gene="guaC"
                     /locus_tag="ACHUU3_000304"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR2080483.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(36854..36898)
                     /locus_tag="ACHUU3_000305"
     CDS             complement(36854..36898)
                     /locus_tag="ACHUU3_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2080484.1"
                     /translation="MIIKTLKMSARKRN"
     gene            36923..37543
                     /gene="coaE"
                     /locus_tag="ACHUU3_000306"
     CDS             36923..37543
                     /gene="coaE"
                     /locus_tag="ACHUU3_000306"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR2080485.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37543..38286
                     /gene="zapD"
                     /locus_tag="ACHUU3_000307"
     CDS             37543..38286
                     /gene="zapD"
                     /locus_tag="ACHUU3_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR2080486.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38296..38493
                     /gene="yacG"
                     /locus_tag="ACHUU3_000308"
     CDS             38296..38493
                     /gene="yacG"
                     /locus_tag="ACHUU3_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR2080487.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38593..38982)
                     /gene="mutT"
                     /locus_tag="ACHUU3_000309"
     CDS             complement(38593..38982)
                     /gene="mutT"
                     /locus_tag="ACHUU3_000309"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR2080488.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(39042..41747)
                     /gene="secA"
                     /locus_tag="ACHUU3_000310"
     CDS             complement(39042..41747)
                     /gene="secA"
                     /locus_tag="ACHUU3_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR2080489.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41809..42321)
                     /gene="secM"
                     /locus_tag="ACHUU3_000311"
     CDS             complement(41809..42321)
                     /gene="secM"
                     /locus_tag="ACHUU3_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR2080490.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42552..43469)
                     /gene="lpxC"
                     /locus_tag="ACHUU3_000312"
     CDS             complement(42552..43469)
                     /gene="lpxC"
                     /locus_tag="ACHUU3_000312"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR2080491.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43570..44721)
                     /gene="ftsZ"
                     /locus_tag="ACHUU3_000313"
     CDS             complement(43570..44721)
                     /gene="ftsZ"
                     /locus_tag="ACHUU3_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR2080492.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44782..46044)
                     /gene="ftsA"
                     /locus_tag="ACHUU3_000314"
     CDS             complement(44782..46044)
                     /gene="ftsA"
                     /locus_tag="ACHUU3_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR2080493.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(46041..46871)
                     /gene="ftsQ"
                     /locus_tag="ACHUU3_000315"
     CDS             complement(46041..46871)
                     /gene="ftsQ"
                     /locus_tag="ACHUU3_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR2080494.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46873..47793)
                     /gene="ddlB"
                     /locus_tag="ACHUU3_000316"
     CDS             complement(46873..47793)
                     /gene="ddlB"
                     /locus_tag="ACHUU3_000316"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR2080495.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47786..49261)
                     /gene="murC"
                     /locus_tag="ACHUU3_000317"
     CDS             complement(47786..49261)
                     /gene="murC"
                     /locus_tag="ACHUU3_000317"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR2080496.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49315..50382)
                     /gene="murG"
                     /locus_tag="ACHUU3_000318"
     CDS             complement(49315..50382)
                     /gene="murG"
                     /locus_tag="ACHUU3_000318"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR2080497.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50379..51623)
                     /gene="ftsW"
                     /locus_tag="ACHUU3_000319"
     CDS             complement(50379..51623)
                     /gene="ftsW"
                     /locus_tag="ACHUU3_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR2080498.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51623..52939)
                     /gene="murD"
                     /locus_tag="ACHUU3_000320"
     CDS             complement(51623..52939)
                     /gene="murD"
                     /locus_tag="ACHUU3_000320"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR2080499.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52942..54024)
                     /gene="mraY"
                     /locus_tag="ACHUU3_000321"
     CDS             complement(52942..54024)
                     /gene="mraY"
                     /locus_tag="ACHUU3_000321"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR2080500.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(54018..55376)
                     /gene="murF"
                     /locus_tag="ACHUU3_000322"
     CDS             complement(54018..55376)
                     /gene="murF"
                     /locus_tag="ACHUU3_000322"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR2080501.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLNAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55373..56866)
                     /gene="murE"
                     /locus_tag="ACHUU3_000323"
     CDS             complement(55373..56866)
                     /gene="murE"
                     /locus_tag="ACHUU3_000323"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR2080502.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56853..58619)
                     /gene="ftsI"
                     /locus_tag="ACHUU3_000324"
     CDS             complement(56853..58619)
                     /gene="ftsI"
                     /locus_tag="ACHUU3_000324"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR2080503.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58635..59000)
                     /gene="ftsL"
                     /locus_tag="ACHUU3_000325"
     CDS             complement(58635..59000)
                     /gene="ftsL"
                     /locus_tag="ACHUU3_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR2080504.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58997..59938)
                     /gene="rsmH"
                     /locus_tag="ACHUU3_000326"
     CDS             complement(58997..59938)
                     /gene="rsmH"
                     /locus_tag="ACHUU3_000326"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR2080505.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59940..60398)
                     /gene="mraZ"
                     /locus_tag="ACHUU3_000327"
     CDS             complement(59940..60398)
                     /gene="mraZ"
                     /locus_tag="ACHUU3_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR2080506.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14597 a        16851 c        15000 g        14509 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgaca gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2701 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2761 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2821 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2881 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2941 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gtgccagctc
     3001 cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     3061 ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
     3121 catggcagca atagaataac gcatcatgcg aaagaccatt ttcttatccg gcttgctttg
     3181 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     3241 gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     3301 gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3361 cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg tactcggtgc
     3421 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
     3481 catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt caattggtta
     3541 cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa tgccgtcaga
     3601 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
     3661 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3721 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3781 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
     3841 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3901 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
     3961 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
     4021 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     4081 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
     4141 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     4201 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     4261 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
     4321 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4381 atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa catcgcgaat
     4441 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4501 acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt gtttcaatac
     4561 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4621 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4681 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4741 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4801 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4861 acggacctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4921 caaattgtcc gccaataccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
     4981 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     5041 gtcttcaact ttttgtcgcc cctgaaccgg ctcaagatgc cctttatcga taaacccttt
     5101 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     5161 tccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
     5221 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     5281 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
     5341 cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca aattatgttt
     5401 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5461 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
     5521 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5581 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
     5641 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5701 taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
     5761 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5821 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5881 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5941 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     6001 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
     6061 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     6121 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     6181 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
     6241 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     6301 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6361 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6421 ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc attggtgatc
     6481 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6541 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6601 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6661 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6721 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6781 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
     6841 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6901 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6961 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
     7021 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     7081 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     7141 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     7201 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatatt
     7261 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     7321 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7381 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7441 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7501 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7561 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7621 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7681 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7741 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7801 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7861 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7921 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7981 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     8041 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     8101 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     8161 gtgtgaactg aacgtcatgg aacaggttta taacctaggc cactccacca ttatgcaatc
     8221 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     8281 cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc aacgttaccg
     8341 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8401 atgcaacaca tccggcaact tcacacttac tcgtcccgca gaatcacttt gccgatatac
     8461 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8521 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
     8581 agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8641 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8701 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8761 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8821 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8881 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8941 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     9001 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     9061 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     9121 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     9181 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     9241 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     9301 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa acgcgccgcg
     9361 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9421 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9481 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9541 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
     9601 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
     9661 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
     9721 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
     9781 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
     9841 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
     9901 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
     9961 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    10021 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc gattaacgct
    10081 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    10141 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    10201 accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    10261 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc cggcgcgaaa
    10321 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcctgg
    10381 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10441 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10501 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10561 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10621 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat
    10681 ggcgaactgg tggttccggc cccggaaaaa ccggtgccgc aaggtgctgc gaaaggcgat
    10741 tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa agatttgagc
    10801 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10861 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    10921 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10981 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    11041 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    11101 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccgtgt
    11161 aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga agtggtgtgg
    11221 aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt accggtgccg
    11281 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    11341 atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11401 tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggc
    11461 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11521 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11581 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11641 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11701 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11761 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg ccgtgaatat
    11821 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11881 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11941 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    12001 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    12061 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    12121 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    12181 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg
    12241 ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg tcgttaacct
    12301 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12361 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12421 aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag ccacgcgggg
    12481 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12541 cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct
    12601 gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12661 cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc
    12721 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg
    12781 tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca cccggtattt
    12841 ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata tcaaccgtta
    12901 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12961 tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat
    13021 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    13081 ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga
    13141 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    13201 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    13261 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    13321 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct gcgcaaacag
    13381 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13441 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13501 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13561 cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg
    13621 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13681 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13741 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13801 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13861 gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13921 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13981 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    14041 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    14101 cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    14161 cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag
    14221 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct
    14281 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    14341 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct atcgcaaact
    14401 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14461 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14521 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14581 tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14641 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14701 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14761 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14821 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14881 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14941 agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg
    15001 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    15061 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    15121 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa
    15181 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    15241 tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata tcttccatac
    15301 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15361 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15421 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15481 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15541 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc ttccatttca
    15601 tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc catccacagg
    15661 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15721 tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa ccagtgcccg
    15781 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15841 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgctatt
    15901 gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15961 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    16021 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    16081 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    16141 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    16201 gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag
    16261 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag
    16321 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc
    16381 aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct
    16441 ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt catgcaagaa
    16501 ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc
    16561 gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16621 gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16681 atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac
    16741 ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16801 gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaact
    16861 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16921 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    16981 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    17041 atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg
    17101 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    17161 agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacacca
    17221 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    17281 tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    17341 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc tacagaagtc
    17401 attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc acgacctacc
    17461 attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17521 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17581 atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17641 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17701 tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg
    17761 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17821 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17881 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17941 tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata
    18001 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac
    18061 gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta
    18121 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    18181 tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag
    18241 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    18301 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt
    18361 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    18421 aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat
    18481 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    18541 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18601 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18661 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18721 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18781 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18841 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18901 cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac cattgcgatt
    18961 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcactaa
    19021 tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc cgcacagtgg
    19081 aagcgcctgt ggaaacgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc
    19141 ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca
    19201 ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac
    19261 aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg
    19321 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19381 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19441 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19501 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19561 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19621 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19681 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19741 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19801 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19861 acgcactatt tagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19921 atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg
    19981 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    20041 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    20101 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    20161 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    20221 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    20281 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    20341 tgacccagca acaaaaggac gccgctcgtc agcgttatca atcggcctct cctgagcagc
    20401 gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga agagaggcag
    20461 cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtgtttaag gaaaaagtac
    20521 agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caatcggcat
    20581 cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa
    20641 acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc
    20701 tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt
    20761 ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aaaaattact tcttcttcgc
    20821 tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga
    20881 ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat
    20941 ttcgattgcc agaccgattt cacccagcag ctcaccgccg ttagtaccga caatcgcacc
    21001 accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc
    21061 ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc
    21121 tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc
    21181 gatagacggg ataactttcg gatcgaagta gtgtttttta ccggcgataa cttcagcggc
    21241 aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccaacga tatcgccgat
    21301 agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc
    21361 gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat
    21421 cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt
    21481 cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt
    21541 gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat
    21601 aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc
    21661 catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag
    21721 ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg
    21781 gatcgggcga gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt
    21841 ctcaccttca acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt
    21901 gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga tcactttctc
    21961 tttccaagta cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc
    22021 agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat
    22081 acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc
    22141 cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac
    22201 cacgacctga gttttgattt cagtactcat catgacctct atatatttat ctccggcggt
    22261 catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt
    22321 acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata
    22381 gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa
    22441 aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc
    22501 aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat
    22561 caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gggcaccatc
    22621 agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac
    22681 gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac
    22741 ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat
    22801 ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga
    22861 aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac
    22921 cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt atttcttcag
    22981 ggtcagacgc tgaccgtctt ccgacagcga actattgaag cgaggcatct gctcaagagc
    23041 tgcagcaacg gctttcatga tgaagacaac cggggtgagc ttcacatcca gcttacgttt
    23101 cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat cggttttgtc
    23161 gaagtgggta acatgcggga tcatcaccca gttacggctc aggttagcac cagaaatttt
    23221 ctggatacgg cccagttcca cttcttcgat ttcgccaaac ttgctgaagt ccaccttcgg
    23281 ccacggcagc atgccaggga taccaccgcc agtcgccgcc ggagctgctt ctgcacgttt
    23341 gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacgaccagt
    23401 gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggagt
    23461 cgcgtgaacg taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt tcgcagccgg
    23521 agctgctgcc ggggcttcag ctttcgctgc cggagccggc gctgccgctt cctgtttcgc
    23581 aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg agccagtttt
    23641 cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccgggac
    23701 ctccatagaa gctttatcgc cttctacggt gataagcgat tgttcagcgg caactttgtc
    23761 gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt
    23821 aacttctttc acgccagccg ctgatgcagg ggccgctgcc ggagctgctt cctgtttagc
    23881 cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt
    23941 agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa acggagccgg
    24001 aacttccata gaagctttat cgccttctac ggtgatcagc gactgttcag cttcaacttt
    24061 atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac
    24121 gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc
    24181 ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag
    24241 tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg
    24301 cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc
    24361 ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat
    24421 gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta
    24481 actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt
    24541 tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc atcaacttcg
    24601 aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg
    24661 cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg
    24721 gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac gcgcggagtt
    24781 tccagcggat gaagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg
    24841 aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc
    24901 gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt acctttgcta
    24961 ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc
    25021 attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt
    25081 ttttcaccgt acatacgctc cagaccgtca tgcatgatga cagcaacttc gtaagcgtaa
    25141 gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct
    25201 tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag
    25261 ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg gaagccgaac
    25321 atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta agaggtcgcc
    25381 gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct
    25441 ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcgggatgta ctgctgaccg
    25501 ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg
    25561 tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat tacgttcagg
    25621 gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag cagcgcgccg
    25681 aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga
    25741 taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga accttccggg
    25801 aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg
    25861 cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct
    25921 tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg
    25981 cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca
    26041 cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga
    26101 tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag
    26161 tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc
    26221 agttcatccc aacggctacc ccacatcact ttgataacgt tccagccagc accttcgaag
    26281 atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc
    26341 aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc gatggtgatc
    26401 gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt
    26461 ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata
    26521 gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc cggcatcagt
    26581 ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc
    26641 tgctcttgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg ggagatgtgg
    26701 ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta
    26761 aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc gcccagttcg
    26821 aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat agctgaacga
    26881 atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg
    26941 ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca
    27001 gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat
    27061 tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct
    27121 gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga
    27181 actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc ttttatatac
    27241 gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg
    27301 ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt cgagcaaaat
    27361 ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc
    27421 cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg
    27481 cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct
    27541 tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag
    27601 tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg
    27661 gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc
    27721 gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag gatggtcgga
    27781 gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc
    27841 gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa
    27901 ggagggacgg gagacgtcga actgttttgc cagttcgcgt tccggtggga gtttttcgcc
    27961 cgggcggaga gtgccttcga gaatcaaaaa ctccagttgc tgctcaatca catcggagag
    28021 ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt
    28081 caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt
    28141 ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata
    28201 acgtttcaaa attgtaacta tgcacaaatg tagactttac gtaggaaagg gattttttaa
    28261 cgattattaa actataaaaa tccgaattga accgattcac ttaccaattt tttgattttt
    28321 aagtcaataa aaacgaattt aaattcattc tgcatattga gaggggttga ggctgagctt
    28381 tacaaacggt ttctttttaa gcaactcatc ttcaaccatg cataaagcgg gtgcattcgc
    28441 tgccgcatac cattattctt gatctgacgg aagtcttttt gtaacaattc aaacttcttt
    28501 gatgtaaaaa aattaataca acaaacggaa ttgcaaactt acacacgcat cactgcgtag
    28561 atcaaaaaaa caatcaccgc acgaggtttc atgatggaag gtcaacagca cggcgagcag
    28621 ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg cgcgataggg
    28681 acagggctat tcctgggtag cgcctccgta atacagtccg cagggccagg gattatcctg
    28741 ggttacgcca ttgctggttt tatcgccttt ctgatcatgc gtcagctggg tgaaatggtg
    28801 gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt ataaatactg gggcagtttt
    28861 gccggtttcg cctctggctg gaactactgg gtactgtacg ttttagttgc catggcagag
    28921 ctgactgccg tgggtaaata cattcagttc tggtatccgg aaatcccaac ctgggtttct
    28981 gccgccgtat tctttgtggt gattaacgcc atcaacctga ccaacgttaa agtgtttggc
    29041 gagatggagt tctggtttgc cattatcaaa gttattgcga tagtagcgat gatcatcttc
    29101 ggcggctggc tgctgttcag tggcaacggc ggcccgcagg cgagcgttag caacctgtgg
    29161 gatcaaggtg gtttcctgcc gcacggcttc accggactgg tgatgatgat ggcgattatc
    29221 atgttctcgt tcggtggtct ggaactggtg gggatcaccg cagcagaagc tgataacccg
    29281 gagcaaagta tcccgaaagc gactaaccag gttatctacc gcatcctgat tttctatatt
    29341 ggttcgttag ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc cgataccagt
    29401 ccgtttgtgc tgatcttcca cgagttaggc gatacctttg tggcgaatgc gctgaacatc
    29461 gtggtactga ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa cagccgtatg
    29521 ctgtttggtc tggcacaaca gggtaatgcg ccaaaagcgc tggcgtctgt cgataaacgc
    29581 ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta cagcattgtg cgtattgatt
    29641 aactatcttg ctccggaatc cgcatttggc ctgttaatgg cactggtggt atccgcactg
    29701 gtgatcaact gggcgatgat cagtctggcg catatgaagt tccgtcgcgc caagcaggaa
    29761 caaggcgtgg taactcgctt ccctgctctg ctttatccgc tgggtaactg gatctgcctg
    29821 ctgtttatgg cggcggtact ggtgattatg ctgatgaccc caggaatggc gatttcggta
    29881 tacctgatcc cggtatggct gatcgtgtta ggtatcggct atctgtttaa agagaaaacc
    29941 gccaaagccg taaaagcgca ttaatctctc tacgccctca cccatgcagg gtgagggcaa
    30001 taatctttac accaacgctc cgtaaatcgt cagtagcgca atcaccacca cgaccacgaa
    30061 cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc tccaccttat cgacatgcgg
    30121 ttcacgcgcc agagagaact gcgccagacg cgttaacacc tgatactgcg aagtatggaa
    30181 gtcacccagc gaagcaaacc aggccggtaa cgctttctcc ccatgaccga tcaaggcata
    30241 taccacaccc gcaagacgaa ccggcaccca atccagtaca tgaagcacgg catcaatgcc
    30301 ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc cagtattgcc atgcacgcaa
    30361 aaacgcatac cccatcagcg taacgggtcc ccaggttccc cccacaatca accagaacag
    30421 cggtgcaaga taaaaacgaa agttaatcca cagcaatgca ttttgcagct cacgcaaata
    30481 ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt
    30541 ggcacgggca tggctatcat tacgtgaagc agctgtcaga taagcatgat aatgaagacg
    30601 aactttacct gcgccaatac acagcaaacc aatcagcagc cacaccagca gcgtgggaac
    30661 gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa gtcacgccca tcgcaataat
    30721 ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag
    30781 acgatgatca agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag
    30841 taaggttgta aatagcgtca tgttgtctcc ttgctgacca gcgcacgaaa ccgtgcccaa
    30901 tcaaatgcag gaccgggatc ggttttccga tccggcgcaa tatcacaatg gcccgtcatg
    30961 ttattagcga tatcgggata gcgatctatc agtgcccgcg taaccgccgc aagctgttga
    31021 tactgcgcat cggtatacgc cagagtatcg gtgccttcaa gctcaatccc aatagaaaaa
    31081 tcattgcagc gttcacgccc ctgatactga gagactcccg catgccaagc acgtttatcg
    31141 aaaggaacat actggactat ttcaccatca cggcgaatca aacagtgagc ggagacgcgc
    31201 aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat caatagttcc agtgaataat
    31261 gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc taatattgtg caccaccagc
    31321 agggtgggtg tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg
    31381 ccaaccagcc acccctgttc taacaacatg caggatctcc ttatatgtgg tgctaatacc
    31441 cggttcagag tagcatgttt ctaccttatg attcgttagc tatctggagt tttaacatgc
    31501 cgcctcgccg ctataaccct gacacccgac gtgacgagct gctggaacgc attaatctcg
    31561 atatccccgg cgcggtggcc caggcgctgc gggaagattt aggcggaaca gtcgatgcca
    31621 acaatgacat tacggcaaaa cttttaccgg aaaactctcg ctctcatgcc acggtgagca
    31681 cccgcgagaa tggcgtattt tgcggcaaac gctgggttga agaggtgttt attcaactgg
    31741 caggcgacga tgtcaccata atctggcatg tggatgacgg cgatgtcatc aacgccaatc
    31801 aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa cgcactgcgc
    31861 ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt acgccactat gtcgaattac
    31921 tggaaggcac caacacgcag ttgttggata cgcgcaaaac cttacctggc ctgcgttcag
    31981 ctctgaaata cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg ctttctgatg
    32041 ccttcctgat caaagaaaat catattattg cctccggctc agtgcgccag gccgtcgaaa
    32101 aagcgtcctg gctgcacccg gatgcgccag tagaagtcga agtagagaat ctggaagaac
    32161 ttgatgaagc cctgaaagca ggagccgata tcatcatgct ggataacttc gaaacagaac
    32221 agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg tctggcaacg
    32281 tcactgacaa aacactgcgc gaatttgccg aaacgggcgt ggactttatc tccgtcggtg
    32341 cgctaactaa acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa ttgctgacag
    32401 atttatccgc tccatcgcgg gcggataatc tccccttccc caactttctt cgtaacgcct
    32461 cgcaaatttc ttgtcttttg ctgcaacgaa ataaaaaacc tcggaagctg ctttcccgtt
    32521 tcgttttttg cccctcgctt gtgctgatgg cgtttgccaa agtagcgcca accaaatcaa
    32581 ggagcgaaac agatggacaa gcaacgcggt tttacactta tcgaactgat ggtggttatt
    32641 ggcatcattg ccattttaag cgccattggt attcccgctt atcaaaacta cctgcgcaaa
    32701 gctgcactca ccgacatgct acaaaccttt gtgccttacc gtacagccgt agagttgtgc
    32761 gcgctggaac atggtggatt agatacctgc gacggtggca gcaatggcat tccctcgcct
    32821 accaccaccc gctatgtttc agccatgagt gtggcaaaag gcgtggtgtc gctgaccggg
    32881 caagaaagtc tcaatggact aagcgtcgtc atgacaccgg gttgggataa cgcaaacggc
    32941 gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca gcgcattgca gcaagcctgc
    33001 gaagatgtct tccgctttga tgacgccaac taaggagcgg caatgaatat tccacagctc
    33061 accgccctgt gtctgcgtta tcagggagtc ttgctggatg ccagcgaaga ggtggttcat
    33121 gttgcggtag tcgatgcacc ttcgcatgag ttgctggacg cattgcattt cgctaccacc
    33181 aaacgtattg agatcacctg ctggacgcgc caacaaatgg aaggccacgc cagtcgcaca
    33241 caacagacat tgcccgtagc tgttcaggag aagcatcagc ccaaagcaga gttgctgact
    33301 cgaacgttac aatctgcgct ggaacaacgt gcgtctgata ttcatatcga accagcggac
    33361 aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc atcctttacc ggatgtttca
    33421 ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc tgggaaacct ggatattgcg
    33481 gaacatcgcc tgccgcagga cgggcaattc actgtcgaac tggcaggaaa cgccgtctca
    33541 tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg tggtattaag gttgttacag
    33601 caggtgaacc aggcactgga tgttaacacg ctgggaatgc agccgttaca actggcggac
    33661 tttgctcatg ccttgcaaca accacaggga ctggtgctgg taactggccc tacaggcagc
    33721 ggcaaaacgg tcacgcttta tagtgccctg caaaagctga ataccgctga cattaatatt
    33781 tgtagcgtcg aagatccggt tgagatcccc atagccggac taaaccagac gcaaatccat
    33841 ccgcgtgccg ggctcacctt tcagggcgtt ttgcgtgcgt tattgcgcca ggatcctgac
    33901 gtcatcatga tcggagagat ccgcgatggc gaaacggcag aaattgccat taaagccgcg
    33961 caaaccggtc acctggtgtt gtctacccta cacactaatt ccacctgcga aacgctggta
    34021 cgtttacagc aaatgggagt cgcccgctgg atgctctcat cagcgcttac gctggtaata
    34081 gcccagcgtc tggtacgtaa actttgccca cattgtcgcc agcagcaagg ggagcccatc
    34141 catattccag tcaatgtatg gccgtcgccg ctgccccact ggcaggcacc cggttgtgta
    34201 cattgctacc acggttttta tggtcgtacg gccttatttg aagttctgcc cataacgccg
    34261 gtcattcgtc agcttatttc cgctaatacc gacgttgaat cgctggaaac gcacgcacga
    34321 caggcgggta tgcgtacgct ttttgaaaac ggctgcctgg ccgtggagca aggcttaacc
    34381 acctttgaag agttaatccg cgtactgggg atgccgcatg gcgagtaagc aactctggcg
    34441 ctggcatggc ataaccggcg acggcaatgc gcaagatggg atgctatggg cagagagccg
    34501 tgctttgctg ctcatggcac tacagcaaca gatggttacc ccacttagcc tgaagcgaat
    34561 cgccatcaat tctgcgcagt ggcgaggaga taaaagcgcg gaagtcattc atcaactggc
    34621 gacgctactc aaagccgggt taacgctttc tgaagggctg gcactgctgg cggaacagca
    34681 tcccagtaag caatggcaag cgttgctgca atcgctggcg cacgatctcg aacagggcat
    34741 tgctttttcc aatgccttat taccctggtc agaggtattt ccgccactct atcaggcgat
    34801 gatccgcacg ggtgaactga ccggtaagct ggatgaatgc tgctttgaac tggcgcgtca
    34861 gcaaaaagcc cagcgtcagt tgaccgacaa agtgaaatca gcgttacgtt atcccatcat
    34921 cattttagcg atggcaatca tggtggttgt ggcaatgctg cattttgttc tgccggagtt
    34981 tgccgctatc tataagacct tcaacacccc actaccggca ctaacgcagg ggatcatgac
    35041 gctggcagac tttagtggcg aatggagctg gctgctggtg ttgttcggct ttctgctggc
    35101 gatagccaat aagttgctga tgcgccgacc gacctggctt atagtgcggc agaaattgct
    35161 gttacgcatc ccgattatgg gttcactgat gcggggacaa aaactcacgc agatctttac
    35221 gattctggcg ctgacacaaa gtgcaggcat tacttttttg cagggcgtag agagcgtcag
    35281 agaaacaatg cgctgcccgt actgggtgca acttctgaca caaatccagc acgatatcag
    35341 taacggtcat cccatctggc tggcgctaaa aaataccggg gagtttagcc cgctctgttt
    35401 gcaattagtg agaacaggag aggcatccgg ctcgctggac ctcatgttag acaacctcgc
    35461 ccatcatcat cgggataaca caatggcgct ggcggataac ctcgcagcct tactggaacc
    35521 gacgttgctg atcataacgg gaggaattat cggtacgctg gtggtggcga tgtatctgcc
    35581 aattttccat ttaggcgatg cgatgagtgg gatgggataa cgctggcgtg ttgctccaca
    35641 ccagcgttgg aagattacag gttgttgaag atgcggtttt cttgttcctg cacacggata
    35701 aatgtggtgc gcttggtcag ttctttcagg cgtgaagccc caacgtatgt acaagctgaa
    35761 cgcaggccgc ccaaaatatc acgcgcggta ttttcaaccg ggcctcgcag cggcagctta
    35821 acggttttac cttctgctgc gcgatattcc gcaacgccgc caacgtgacg tttcatcgca
    35881 gactcggagc tcatgccgta gaacagcata aatttctcgc cgttctcctc aacgatgcga
    35941 ccgccgctct cttcgtggcc cgccagcatg ccgccaagca tgacgaaatc ggcaccgccg
    36001 ccaaaggctt ttgcgacatc gcccggcgtg gtgcagccac cgtcgctgac gatcattccg
    36061 cccagaccgt gcgccgcatc ggcacattcg attaccgcag aaagctgcgg ataaccgacg
    36121 cctgttttga cgcgagttgt acaaacagaa cctgggccaa tgccaacttt aacgatatca
    36181 gcaccggaga ggataagctc ctcacacatt tcaccagtca ctacgttgcc agcacaaatg
    36241 gttttggtcg gccacgcttc acgcgctttc gcaacgaact gcacgaagtg ttcggaataa
    36301 ccattcgcca catcaataca aacgaagttt aatgccgggt tcaggtcgag aatctgttta
    36361 gttttttcga aatccgcatc agacgtaccg gtagaaacca tcacatgttt cagcacatca
    36421 gcggaagaat tgttgataaa cgcttgccac tcttcgacag aatagtgttt atgcacagca
    36481 gtcaaaatat caaaagaagc cagcgcagag gccatagaaa atgtacctac ggtgtccata
    36541 tttgcggcga taatcggcac gccggaccag ctctgacctg aatgtttgaa ggtgaattga
    36601 cgttccagtt caacatcgga acggctttta agagtggagc gtttagggcg gatgagaacg
    36661 tctttaaaac ctaacttcag atcttcttca atacgcatgt gcggattcct ggggttaatg
    36721 gctataaggg taagagcgca actccagtga cgctatcata cgcactaatc aatgcggcgc
    36781 aagactgcga aattgtcttt ttttacgcta caatcccata aatttacctg gtaaatagtg
    36841 agcgaacgtt tcatcaattt cgctttcgtg cactcatttt taacgttttg ataatcatga
    36901 tgaattcccg ggaatataaa ttatgaggta tatagttgcc ttaacgggag gcattggcag
    36961 tggcaagagt accgttgcca atgcgtttgc tgatctcgga attaacgtca ttgatgccga
    37021 tattattgcg cgtcaggtgg ttgaaccagg tgcacctgcg ctacatgcca ttgctgatca
    37081 ctttggcgct aacatgattg ctgctgatgg aacattgcag cgccgggcct tgcgcgagcg
    37141 gatcttcgcc aacccggaag agaaaaactg gcttaacgcc ctgctgcatc cgctgattca
    37201 gcaagagacg caacaccaga tccagcaagc tacttccccc tatgtactgt gggttgtgcc
    37261 attgctggta gaaaactcac tgtataaaaa agcgaatcga gtgctggtgg tggatgtcag
    37321 cccagaaacg caacttaagc gcaccatgca gcgcgatgat gtaactcgcg agcatgtcga
    37381 acaaatcctt gctgctcagg caacgcgcga agcccgcctt gccgtggcag atgacgtcat
    37441 tgataataac ggcgcaccgg atgctatcgc atcggatgtt gcccgcctgc acgcacacta
    37501 tttgcagctt gcgtcgcagt ttgtctcaca ggaaaaaccg taatgcagac ccaggtcctt
    37561 tttgaacatc cactaaatga aaaaatgcgt acatggctgc gcattgagtt tttgattcag
    37621 caactcaccg ttaatttacc catcgttgac cacgctggcg cgctgcattt cttccgtaat
    37681 gtcagtgaat tactggatgt tttcgagcgc ggcgaagtcc gcactgagct gttgaaagaa
    37741 cttgaccggc agcaacgtaa actccagacc tggattggcg tgcctggcgt ggaccagagc
    37801 cgtattgaag cattaattca gcagttaaaa gcggcgggga gcgtattaat ttccgcgccg
    37861 cgtatcgggc aatttctgcg tgaagatcgt ttgattgctc tggtgcgtca gcgactgagc
    37921 atcccaggcg gctgttgcag ctttgattta cctacattgc acatttggct gcatctaccc
    37981 caggcgcagc gcgacagcca ggtagaaacc tggattgcca gcctgaaccc gctcacccag
    38041 gcacttacca tggtgctgga tttaattcgc cagtcggccc ccttccgtaa acaaaccagt
    38101 ctgaatggtt tttatcagga taacggtggc gatgccgact tgctgcgcct gaatctgtcg
    38161 ctcgattcac agctttatcc gcaaatttcc ggacataaga gccgttttgc cattcgtttt
    38221 atgccgctgg acactgaaaa cggacaggta ccggaacgtc tggatttcga actggcctgt
    38281 tgctaaggag taaaaatgtc agaaactatt acggtgaatt gcccaacctg cgggaaaacg
    38341 gtggtgtggg gtgaaatcag cccgtttcgg ccattttgct ccaaacgttg tcagctgatc
    38401 gacctcggag aatgggctgc tgaagaaaaa cgaatcccca gcagtggcga tctttccgaa
    38461 agcgatgact ggagcgaaga gccaaagcag tgacatttgt ctgatgccgc acgtaggcct
    38521 gataagacgc ggacagcgtc gcatcaggca tcttgtgcaa atgtcggatg cggcgtgaac
    38581 gccttatccg acctacagac gcttaagctt cgcaattacc ggttcattgg ctggcggaaa
    38641 atcatcggca ttaagaccga ctagcgacat ccactcaccg ggttgccctt ctttacccca
    38701 cggctcccct tcccagcttt cgaccagcca gaaccacaga gttatatgcc tgtccgggaa
    38761 ttcatattcc agtttttcaa atagcgaaaa atgttgtggg gtaatcccga cttcttcctg
    38821 aagttcacgc actaccgcct gttccggcgt ttcgcccatt tcaattttac cgccgggaaa
    38881 ctccagttta ttcgccatgt gcgcatctgc tgcgcgacgc gttataaaga tttcattgtt
    38941 ctcgttgcga ataataccta ccgcaatttg cagctttttc attgtcttaa acctataaaa
    39001 aaggcgcaga atcctgcgcc ttttacttca actgttagct tttattgcag gcgaccatgg
    39061 cactgcttgt attttttacc agaaccgcac gggcaaggat cgttacgtcc tactttgcgc
    39121 tcaccggttt gcgccgccag tgcagctgcg gctgcagagt cgtcatcctg atggctaagc
    39181 tgctgcattt gcgctaaacg ctcggcttcc atacgacgct gttgttccag ctcctcaacc
    39241 tcttcaggca tacgtacctg gactttgctc agcgtgctga taacttcata ttttaacgac
    39301 tccagcatcg ctgcaaacat ggagaacgat tcacgtttgt attcctgctt cggatctttc
    39361 tgtgcatagc cacgcaggtg gataccctga cgcagatagt ccatcgccgc taggtgctct
    39421 ttccacagag agtcgagagt ttgcagcatg acgccttttt cgaagtgacg catcatctca
    39481 gcaccaacca cttcttcttt acgctgatac acttcgatgg actgcgccag aattcgctca
    39541 cgcagcgtct cttcatgcag ttctggttct ttatccagcc actcggcaat tggcaaatcg
    39601 aggtcgaaat cgttcttcag acgttcctgc agccccggaa tatcccacat ttcttccagt
    39661 gactgtggcg gaatgtaggc atcaatggtc gctttgaaca catcttcacg aatgctgttg
    39721 atggtttcgc tcacatcgct gacatccagc agttcgttac gctgggagta aatggcgcga
    39781 cgctgatcgt tagccacgtc atcatattcc agcagttgct tacgaatgtc gaagttacgg
    39841 ctttcaactt tacgctgggc gttggcaatc gctttagtca cccacgggtg ttcaatggct
    39901 tcgcctggct tcatacccag tttacgcatc atgccggata ctcggtcgga agcaaaaata
    39961 cgcatcagcg catcttccat cgacaggtag aaacgggaag aaccagcatc cccctgacga
    40021 ccagaacgac cgcgcaactg gttatcgata cgacgggatt cgtgacgctc ggtaccgatg
    40081 atatgcaggc cacctgcttc cagtaccgca tcgtgacgta cctgccagtc ggctttaatt
    40141 ttttcaattt gctctgcggt cggattttcc agcgcggcaa cttctgcctg ccagctacca
    40201 ccgagcacaa tatctgtacc acgacccgcc atattggtcg cgatagtcac cgcagccgga
    40261 taacctgcct gagcaacaat cgccgcttcg ttggcgtgga atttggcgtt caggacgttg
    40321 tgcttaatac cggctttggt cagttcgttt gacaccagct ccgatttttc gatggagata
    40381 gtacccacca gcaccggctg gcctttcgca gtacgttctt tgatatcttc aatgatcgcc
    40441 tgaatttttt ccgcttcagt catgtagacc aggtccggca gatctttacg aatcattgga
    40501 cggttggtcg gaacaacgac ggtatccagc ttgtagatgg agctaaattc gaaagcttcg
    40561 gtatcagcag taccggtcat ccccgccagt ttttcataca gacggaagta gttctggaag
    40621 gtgatcgaag ccagcgtttg gttttcgttc tggatctgca caccttcttt cgcttccaca
    40681 gcctggtgca gaccatcgga ccagcgacgg ccctgcatgg tacgaccggt gtgttcgtca
    40741 acgatgataa cttcaccatc tttaacgatg tagtcgacgt cgcgggtaaa cagcgcatga
    40801 gcgcgcagcg ccgccgttac gtggtgcatc agcatgatgt tggccggaga gtacagagac
    40861 tccccttcat ccatgatgcc ctctttcacc agcagttctt caatcagcac cagaccacgt
    40921 tcggtcaggt tcacctggcg agatttttcg tccaccgaga agtggccttc gccctggaag
    40981 gtttcggagt cttctttttc ctgacggatc aggtgcggaa taattttatt cacgcgttta
    41041 tacatttccg agctgtcttc tgccgggccg gaaatgatca gcggtgtacg cgcttcatcg
    41101 atcaggatgg agtccacttc gtccaccagc gcatagtgca gtttacgctg tacacgttct
    41161 tcagggctga acgccatatt gtcgcgcagg tagtcaaagc cgtattcgtt gttcgtaccg
    41221 taagtgatgt cagctgcgta agcttcgcgc tttgccggtg ctggcatgcc cggcaggttg
    41281 ataccgacag tcaggccaag gaattcaaac agcggacggt tgttttcggc gtcacgttgc
    41341 gccaggtagt cgttgacggt aactacgtgc acgcctttac cggttagtgc gttcaggtaa
    41401 gcaggcagcg ttgcggtcag ggttttacct tcaccggtac gcatttcggc gatgcagcgt
    41461 tcgttaagaa ccataccgcc gagtaactga acgtcgaagt gacgcatacc aaagacgcgc
    41521 ttacttgcct cacgtaccac ggcgaaagct tccgggatca gattttccag cacttcgcct
    41581 ttttccagac gtgcacgaaa ctctgcggtt ttccctttca gttcttcgtc ggagagtttt
    41641 tccatctccg gttccatggc attgatgatg ttgaccactt tgcgcatccg gcgcagggtg
    41701 cgatcgttac gactaccgaa aactttagtt aacaatttga ttagcataat aaaatctcaa
    41761 acgccccgcg ttgcggagtt aataaaatga agtaaaggtt tattgttgtt aggtgaggcg
    41821 ttgagggcca gcacggatgc cttgcgcctg gcttatccag acgggcgtgc tgaattttgc
    41881 ttgtggggta aaatgcgcat aatcaatgcg ataacccttt tcagacggcg tgccttcctg
    41941 ggtcagcagc gcgctgagcg tatccagtaa tgcaagatgt tgcgcctgaa gaggcaaaga
    42001 ttcttcagca acgggcagtg tttgcggtgc cattgcgaaa gaaagatgac ggattaccgt
    42061 gcgaatggca tgttgatgcc agtaatcaac ggaatagttc gaattcgggc ggcgtgtgtt
    42121 cgcttccagc aaggccaatt gaccaaagtt aactttggct gaaggctcgt ggttgcgggt
    42181 tgtcgctttt gcgggcgcgt ttggttcggc ggcgttgctg agcgcaggca aacctaaact
    42241 cgccgcaacc atccctaata agagatgcgg ccagaagtag cgtttaccaa actgtcgcca
    42301 gcgcgtcagt attccactca cgttattgcc atcccgatcg tattcaaacg tatttagcgc
    42361 acaaatctta tcattaaagc ctgcagtcca cagcacgaat gaagataagt tgtgcggaaa
    42421 agtgcttaag aaaacagcgt tcgcaccgtt ttttatagct cttataaaat tcaaaaaaaa
    42481 atgactggtt cacctggccg gagagagtgc cagatttgcc agtcgaattt tatacgacag
    42541 tataaatgtc gttatgccag tacagctgaa ggcgctttga aggccaacgg cagttctgcg
    42601 tcgtcctgga aggtcacata ttcccaggct tcctgtttcg ccaggacagc ctgcagcagt
    42661 ttgttattca gtgcatgacc ggatttataa gcggtaaatg caccaataat attgtgacca
    42721 cacatgaaca agtcaccgat cgcatcgagc attttgtgac gcacaaattc gtcttcaaaa
    42781 cgcaggccgt cttcgttcag tacgcgataa tcgtcaacaa cgatggcaca atcgaagctg
    42841 ccgcccaggc acaaaccacg ggactgcaga tattcgatat cacgcatgaa accgaacgta
    42901 cgcgcacggc tgatctggcg cataaacgca tcagcggaga agttcatcgc atagcgctgg
    42961 ttgctggaat caatagccgg atggttaaaa tcgatggtga aatccagcga aaaaccattg
    43021 tacggcttaa attcagccca cttatcgcca tcttcgacac gaacagtctc tttgatgcga
    43081 acaaattttt tggcgcagtt caactcgtcg ataccggcgt caagcagcag gtatacaaac
    43141 ggagcggcgc tgccgtccat gatcgggatt tccggcgcgt taacttcgat aacaatgtta
    43201 tcgatgccca ggcccgcgag agcagcattg aggtgctcta cggttgaaat ccgtacatca
    43261 tgctcgttga ccagacacgt acagagcatg gtatcacgca cagatttggc atcggccggg
    43321 aaatctaccg gtggattcaa gtcggtgcga cgatagatga ccccggtgtt ggccggcgca
    43381 gggcgtaacg tcagagtgac tttcttgccg gtatgtaaac cgacacccgt cgcctgaacg
    43441 atacgtttaa gtgtcctttg tttgatcatc gtattatctc gccaaattac ctatccaacc
    43501 gaagtgtact atacattcgg cgggccagtt tagcacaaag agcctcgaaa cccaaattcc
    43561 agtcaattct taatcagctt gcttacgcag gaatgctggg atatccagat aatccggctc
    43621 tttcgcagtt tgcggcgcat tgtcattcac gactttagca accggcttct gctcctgggt
    43681 cagcggagcc atcccatgct gctggtagcg atccatcact ggctgctgaa cctgcttatt
    43741 ggtcaccaga gtgatttcag gacgtttgtc catgccgata cctgtcgcaa caacggttac
    43801 gcgcagctcg tcattcatat ccgggtcaag agaagtaccg ataaccacag tcgcgttgtc
    43861 ggaagcaaat gcacggatgg tgttacctac cgtttcgaac tcatccagac gcaggtcgaa
    43921 gcccgccgtg atgttaacca gcacgccgcg cgcgccagac aggtcgatat cttccagcag
    43981 cggagaagag atagccattt cagcagcttc ttccgcacgg tcttcaccgc tcgccacgcc
    44041 agaacccatc attgcgtagc ccatctcaga cattacggtg cgtacgtctg caaagtccac
    44101 gttcatcaaa cccggacgag taatcagttc agcgatacct tgcacagcgc ctttcagtac
    44161 atcgttcgct gcgccaaacg catccagcag ggagataccg cggcccagaa ctttcagcag
    44221 tttgtcgttc gggatagtga tcagagagtc cacatgcttg gacagttcag tgatcccctg
    44281 ctccgcgaat gccatacgct tcttgccttc aaagttgaaa ggcttagtga cgacagcaac
    44341 ggtcaggata cccaaatctt ttgccacttc agcgacgact ggtgctgcac ctgtaccggt
    44401 accaccaccc atacccgcag caataaagac catgtctgca ccttccagcg ccgcacgcaa
    44461 tgcatcgcga tcctcatcag ccgcattgcg gccaacttct ggattagcgc cagcgcccag
    44521 tcctttggtg ataccgctac cgatttgaat cgtctgtcca accgctgttt tacgcagcgc
    44581 ttgtgcatcg gtatttaccg cgaagaattc aacaccttca atacgctcgc gcaccatgtg
    44641 ttcaacagca ttaccgccgc cgccgccgac gccgatgact ttaatcaccg cgtcattggt
    44701 aagttccatt ggttcaaaca tagtttctct ccgatttgtg cctgtcgcct gaggccgtaa
    44761 tcatcgtcgg cctcataaaa attaaaactc ttttcgcagc caactattga gtcgcttgat
    44821 ccacgagcca actgatgctg taacacgttt ttctacttca gcttcaccgt taagatgtga
    44881 ctctttccca tagtgaagca atcccaccgc cgtcgaataa tacggctcct gagcataatc
    44941 cgttaaaccg gtaatgttca gcggcgcgcc gatacgcact tgcgtatgaa acacgcgctg
    45001 agcacaggct gcaagacctt cgatctgcgc tgcgccaccg gttaatacaa tgcctgccgc
    45061 caggtgatgt ttaacccctt gttggcgaag cttttcctgc aactgcaata tctcttcgtt
    45121 gaccaggttg agcagctcgg tatagcgcgg ctcgatcacc tctgccaggg tctgacgttg
    45181 cagactccgt ggcggacgac cgcctacgct cggcacttcc acgctctcat cttttccaac
    45241 gatggaaccc agcgcacaac cgtggcgaac tttaatcgct tcggcgtcgc ttggcggcgt
    45301 gccaaaggcg taagcgatat cactggtcac gacattgcca gcataaggaa ttaccttagt
    45361 gtggcgcaat gccccaccgg tataaacggc gatatccatt gtaccaccac cgatatcgac
    45421 gacgcagaca cccagttcac gttcatcttc cgtcaatacc gaataacttg atgccagtcc
    45481 ggcaaatatc agttggtcaa ctttcagccc acaacgttca accgctttga cgatgttttt
    45541 cgccatatcg ttgtgacatg tgatcaggtg cacttttgcc tgcatccgca cgcccgaaag
    45601 tcctaccgga ttcttgatcc cttcctgata gtcaatcgca tactcttgcg ggatcacatg
    45661 cagcacacga tgttcatcgc gcacacgcac cgatttcgcg gtatggacga cgttttccac
    45721 atcttcttgc gtcacttctt cttcagaaat aggcaccata ccaatttcat tctggcagct
    45781 gatgtgctta ccagaaagcg ccagatatac cgaagagatc tgacaatctg ccatcaattc
    45841 tgcctggtca atggcgcgtt gtacgcactt gaccacggat tcgaggtcgt tcaccccgcc
    45901 tttatccata ccacgtgacg ggcagctgcc cacgccaatg atattgacca taccgtcggg
    45961 cagaacttcc cctactaaag cggcaacctt cgcggtacca atctccagtc ctactaccag
    46021 ttttctgtcc gtcgccttga tcattgttgt tctgcctgtg cctgattttg ttgctgagta
    46081 gattcctctg gcggcaaggg cgcccagcct actgccgctc cagagtcata acgcaaatca
    46141 acgtagctaa tccgtttgcc atcggtttgc gcctgctgct gtaaaaccgg ataaagttct
    46201 acaaagcgag ccaaacgttt catcgtatcg ccccggccaa gattgagctt aatatcgtta
    46261 ttcagcgtca actgccagga acgccgcgcg gtcatcgccg cttccttcag agtaaatctg
    46321 tcctttgcca gcatctgccc catttcgcga tagccctgca acacttcatt ggcgctgcct
    46381 tccgggccat acagcattgg aagcacctgc ttgctggtgc gatctggcgg cacgctgaag
    46441 gtgtttcctt ccgcgtctac catatgttga tcattccacc gcgcaatcgg cacatattca
    46501 accagatgaa tcttcaattc atcaggccac tgctttctga cgctcacctg cttaatccac
    46561 ggcaggcgtt gttctatttg cgtctggatg atgttgacat cctgggtcat aaaggtaccc
    46621 ggctcaccca atgccaggat cgactgccgg atatcgtcat tacgcgtgta atggcgttca
    46681 ccggtcaaca ccagctttga gagcggcagg cgttgcgcat cttccatcca gcccaacacg
    46741 acccagccgc tcaccaacac tgtcgttaaa acggtcagca ggaaaaggat ccccgccaga
    46801 cgcgttccat tattgcggcg agaagaaacc tcttcttcgc tatttcgcgt gttcagagca
    46861 gcctgcgaca tattagtccg ccagttccag aattcgtact accaactgcg agaagctcat
    46921 acctgcctga cgtgccgcca tcggcaccag gctgtggctg gtcatacccg gtgaggtatt
    46981 ggcttccagc agataaaact gtccatcgct gtccagcata acgtcaatac gtccccatcc
    47041 tttgcaacct aacgtcgtcc atgctttcag cactaatgcc tgcaaattgg cctcttgtga
    47101 cgcttccaga cctgcggggc agaaatactg tgtctcatca gagagatact tcgcctcata
    47161 atcatagaag gttccggacg gttgaatacg tattgacggt aaaatttctt caccgagtat
    47221 cgcaaccgtg aactccggcc cacttagcca tttttcaatc aatacttctt catcgtgctg
    47281 aaatgccaat cttaatgcat cttgtagagc attttctgct actacttttg acattcccac
    47341 actggaacct tcgcggctcg gcttaacgat aaccggcaaa cccagagcag aaatttctgc
    47401 taactgctta tcgctcaggc ctttttcaaa ctctgcgcgg gttaacgcta cccacggcgc
    47461 gaccggtaaa ccggcacctt gccacagcag tttgctgcgt agtttatcca ttgaaagcgc
    47521 agatgccatc actccgctgc cggtataagg caagcccatc agctcaagca tgccctgcag
    47581 cgtaccatct tcaccgccgc gaccgtgtag cgcgataaac actttctgaa aacccatcga
    47641 cttcagttgc gtcacgtcga cttctttcgg gtcgacagga tacgcatcaa taccgccttc
    47701 acgcagcccg gctaacaccg ctgcgccaga attcagagaa acttcccgct cagcggaagt
    47761 cccgcccaac aggaccgcaa ttttatcagt catgttgttc ttcctccgga gtttgcggct
    47821 tcagtttgat ttcagctaaa gaacgggcaa tttttccgat attaccagcc ccctgaacga
    47881 gaatcaggtc gttaccgatt aataccggtg ccagcatctc ggctacctgc gcagggtccg
    47941 gcaccagaat gggatcaatt ttcccacgtc cacgaattgt gcgacacagc gaacggctgt
    48001 ccgctcccgg aattggcgct tcgccagccg gatacacttc cagcatcaac agggtatcaa
    48061 cctgcgtcag cacattggcg aaatcatcat acaggtcgcg cgtacgggta aaacggtgcg
    48121 gctgaaacag cattactagg tttttatccg gccagcctgc gcgcgccgct ttaatggtgg
    48181 cgtcaacttc cgtcgggtgg tggccgtagt catcgaccag cattgccgta ccgcttttac
    48241 cattcactgg ctccagcggg aattcaccga ggaaatcaaa acggcgacca gtcccctgga
    48301 agctctccag cgcacgcaaa atagcctcgt cgtcaattcc ctcttccgta gcaaccgcaa
    48361 ccgcagctgc ggcgttcagc gcgttatgac ggcctggcgc attcagggtg acgcgcatcg
    48421 gctctttgtc ctggcgcagc agcgtaaagt gcccctgcgg gccaatctgc tgataatctt
    48481 ctacacgcac gtcggcatct tcgctgaagc cgtaagtcgt ggtctgacgt cccacacgcg
    48541 gtaacaattc gcggatcacc ggatcatcaa cacacatcac cgcacgaccg taaaacggca
    48601 ggttgtgcag aaaattaata aaagtctgtt ttaaattctc aaagtcgccc tggtaggtat
    48661 ccatgtggtc ggcttcgata ttggtgacaa tcgccaccat cggttgcaga tgcaggaacg
    48721 atgcatcact ctcatctgct tcggcaatca ggtaccgacc atgccccaaa cgcgcatgaa
    48781 cccccgccgc ttttaccagc ccgccgttaa cgaaggttgg gtcgagcccc gcttctgcgt
    48841 agatgctgga aaccatcgcg gtggttgtcg ttttgccgtg cgttcctgca atggcgatgc
    48901 catgacgaaa acgcattaac tcagccagca tttcggcacg acggatcacc ggaatacgcg
    48961 cttcatgagc tgcgacaatt tccgggttat cggcagaaat ggcgctggaa acaacgacca
    49021 cgctggcatc acgtacgttt tccgggcgat ggttgaaata aatcgtcgct cccagattca
    49081 ttaactgctg cgtgaccgga tttggcgcta aatcggaacc actgatctga taaccttcat
    49141 tggccagaac ttcggcaata ccgcccatac cggcaccacc gatgccgaca aaatgtatgt
    49201 gccgaacgcg acgcatttcg ggcacgatgg aacgcagttt tgccaattgt tgtgtattca
    49261 ttctttacgc cattaacttc ttaaattcat acgatgcaaa aggcatcgct acaattacgc
    49321 ccgggcagcc cggctcactt catttgccac tcgctcggtg gcatccggaa tggatgcagc
    49381 gcgggcgcgt tctgccatgg ttaataaggt ttctcgcgac cacccggcca gggtgttggc
    49441 gacagcatcc acgctaagct gtggctgctc gataattttg gctgcgcccg ctttttccag
    49501 cggtagcgca ttccagtatt gctggcggtc tttatgttga aacggcacaa acaacgccgg
    49561 tagtcctgcc gcggcgattt cactcaccgt taaagcaccg gagcggcaaa cgacgacatc
    49621 cgcccacgca tacgccgccg ccatatcatc aataaattcc gtcactttat gctgcggttg
    49681 ccccgcttcg gcatacgcct gttcaacgga ttgttgcgaa cctttaccgc tctgatgcca
    49741 gatagtgact gaatcaccca gtttcgcagc aacctgcggc attgtctggt taagaatgcg
    49801 tgcgccctgg gaaccaccca ctaccagcac acgaaccgga ccttcacgtc cagccaaacg
    49861 ttgctgcggc aacggcagcg ccagcacatc ggtacgcacc gggttaccca ccacttccgc
    49921 attagggaaa gcgcctggaa acgcctgcat cactttggtg gcaatcttcg ccagccattt
    49981 attggttaag cccgcaatac cgttttgttc atgaagtaca accggaatgc ctaacgacca
    50041 cgcggccaga ccacctggac ctgatacata gccgcccata ccgagtacca cgtcaggttt
    50101 gtacgctttc ataatcgccc gcgcctgacg ccaggcgttg aagatacgca gcggcgcagc
    50161 tatcagtgct tttatacctt ttccacgcag accagagata cgaatgaaat caatttcgat
    50221 gccatgtttt ggcactaagt ccgcttccat acggtcggca gtccccagcc agcgaacttg
    50281 ccaaccctga gccattagat ggtgcgcaac cgccagtccc gggaatacat gtccaccggt
    50341 tccgcctgcc atcaccatta atcgctttcc ttgaccactc atcgtgaacc tcgtacaaac
    50401 gcctgcgctt tctccagacg cgtttcataa tcaatacgca acagcatcat gatggctgtc
    50461 gacataatca gtaagctcga accaccgtaa ctgatcagcg gcaatgtcag acctttggtc
    50521 ggtaacatcc ccgccgccgc gcctacgtta accagcgcct ggaagctaaa ccagatgcca
    50581 atagaacagg cgagaaaacc ggaaaaacgg tggtcaattt ctaacgcttt gcggccaatc
    50641 gacatcgcgc gaaaagcgac gaagaatacc attaaaagtg ccagcaccac accgacatac
    50701 cccagttctt cgccgataat ggcgaaaata aagtcagtgt gcgcttccgg cagatactcc
    50761 agtttttgta ccgagttacc taaaccttgt ccccaaagtt cgccgcgacc aaacgccatc
    50821 agcgattgcg ttaactgata gccgctgcca aagggatctt cccacgggtt ccagaatgcg
    50881 gtaacacggc ggatacggta cggttcggcg agtatcagca acacaaccgc tgaaatgccc
    50941 ataccgataa tggcaatgaa ctgccacaat ttcgctcccg ccaggaacaa catcgccagc
    51001 gtagtcacaa acaacaccac caccgtacca aggtctggct gtgccagcag taacactgcc
    51061 aacaccagaa tcacgcccat cggtttcagg aagccgcgca ggttattacg tacttcgtcg
    51121 cctttacgca ccagatagtt ggcgatataa caaaacagcg acagttttgt cagctccgca
    51181 ggctggatac gcagcaaacc gagatcgatc caacgcgatg cccctttaac cgagctaccc
    51241 actaccagca cgatcatcag caggatgata gatccgagca gcatcgtggc actgtagcgt
    51301 tgccagaact ccatcggcag acgcagcgta atgatcgcca gaatgaatgc caggatcaga
    51361 tagacgccat cacgcttcgc gaagaagaac ggatcgttgg ttaagcgttg ccctatgggc
    51421 attgacgccg aggtcaccat gataaagcca atcgccgcaa ggccgaaggt cagccacagt
    51481 aaggtgcgat cgtacatgat caggctgtcg gtatcttttt cccgcgagcc catcacccag
    51541 ccttttagcg ccgtggagat ccagaccagg atactgaatc ctggcaggcg cggcattttc
    51601 aggcgaggga gagataaacg catcaaccta actccttcgc cagacgggca aactcattgc
    51661 ctcgttgttc aaagttcttg aactgatcaa ggctggcaca ggccggggag agcagaacca
    51721 tatcgcccag ctgaacacgc ggagccagca agcgcatcgc ttgttccatg gtttcggttt
    51781 gttctgccac ttccgggcgt agcgccgcca gctgcgcacc gtcacgacca aaacagtaca
    51841 aacgcacgtt gtcgccattc agatagcgcg ccagtggact gaagtccgcc gatttaccat
    51901 cgccgcccag caacaaatgc agcgtgccgt ctacctgcaa gccattcaac gccgcttccg
    51961 tactgccgac gttagtggct ttcgaatcgt taatccagcg tacgccattg tgctccagca
    52021 ccacttcaaa gcgatgcggc agaccggtga aggtggttaa cgctttcagg ctgctggcgc
    52081 gcggcaaacc agctgcgtct gccagtgcca gtgctgccag cgcattggtg tagttatgct
    52141 gcccggaaag tttcatctct ttcacattca gcactttctc gcctttaacc cgcagccagg
    52201 tttcgccctg ctgatgattc aggtgatagt cacccatgtt gacgccaaag ctgacgcagc
    52261 gttcatcagc accgcgaatc ggcattgtta aggcatcatc agcattgacg acacaaactt
    52321 tcgcattttc gtaaatacgc aattttgccg cacggtattg ctgcaaacca aacggatagc
    52381 ggtccatatg atcttcagtc acgttcagaa tggtcgccgc caccgcctgt aagctggaag
    52441 tggtttccag ctggaagctc gacagttcca gcacgtacag ttcgcactca gcatcaagca
    52501 gcatcaacgc tggcaggcca atattgccac ccacaccaac gttaaccccc gccgctttcg
    52561 ccatttcacc cactagcgtg gtgaccgtgc ttttgccgtt agaaccggta atcgccacaa
    52621 tcggtgcttg tgcttcgcga cagaacagct cgatatcgcc aacgatttcg attccggcat
    52681 cagcggcagc gcttaaggat ggatgcgcct gtgcaatacc gggactggcg acaatcagat
    52741 ctgccgccat cagccattca tcattcagac tgcccgtgtg gcgttctacg gcttcgggta
    52801 atttatccag gccaggcggt gtcatacgcg tatccataac gcgcggcgtc acaccgcgag
    52861 cgaggaaaaa gtccacgcag gaaagcccgg tgaggcccag gccgataatg acgacatttt
    52921 taccctgata atcagccatg attaacgtac cttcagcgtt gccagaccaa tcagaaccag
    52981 catcagcgaa ataatccaga aacgcacaat gacgcgcggt tccggccagc ctttcagttc
    53041 atagtggtga tgaatcggtg ccatgcggaa aatacgttgt ccgcgcagtt taaaggagcc
    53101 gacctgcagg atgacagaaa gcgtttctac cacgaacacg ccccccataa tcaccagcag
    53161 gaattcctga cgtagcagta cggcgataat gcctaacgca ccacctaacg ccagcgaacc
    53221 tacatcgccc ataaagacct gcgccggata ggtgttaaac cacaggaagc ccagtcctgc
    53281 cccgactatc gccgtgcaga caatgaccag ttcccctgcg tgtcgcagat acggtatatg
    53341 caagtagctg gcaaagttca tattgccggt cgcccacgcc accagcgcaa aaccaccggc
    53401 gacaaatacg gtcggcataa ttgccaggcc gtcgagacca tcggtcaggt ttaccgcgtt
    53461 gccagtaccc acaatgacga agtaagccag cagaatgtag aacagcccca gctgcggcat
    53521 cacatcttta aagaatggga ccaccagctg cgttgcgggc gtgtctttgc cggcaaggta
    53581 cagggcgaag gcgacaccca gcgcaatgac cgacatccag aaatacttcc aacgagcgat
    53641 caaccctttg gtgtctttac gcaccacttt gcgataatca tcaacaaagc caataacacc
    53701 gtaacctacc agcaccacca acacgcacca gacgtacgga ttggacgggt aagcccacag
    53761 cagtacggag atcacaatcg ccgtcaggat cataatcccg cccatggtcg gcgtaccgcg
    53821 cttgctgaag tgtgattcag gaccgtcgtt acgcaccacc tgaccaaagg aaagtttttg
    53881 caaatgagct atcatacgcg ggcccatcca caatgagatg aacagcgcgg tcagcaggct
    53941 gacgatggcg cgaaacgtca gataggaaaa gacgttaaag ccggaataat atttgaccaa
    54001 atgttcggcc agccaaacta acatgtccca ttctcctgta aagcgcgtac tacctcttcc
    54061 atggcggcac tacgtgaacc cttaactaaa atcgtaatta cctgttgctc agcaatcagt
    54121 gatttaagac gcgtaattaa cgcagtttta tcagcaaaat gttcgccaac gccgctggcg
    54181 gtgctgatag catggctttg tttacccacg cttaacacgc ggtcaatacc agcagctttt
    54241 gccgcctcgc ccacctgtac atggcaggct tcgctttcag cgcccagttc cgccatatcg
    54301 cccaccacca gcacgcggta gcccggcatt tcagccagta cctgaacagc ggcggtcatt
    54361 gaaccgacat tggcgttgta ggagtcgtcg agcagcaact ggttttctgc cagttggatg
    54421 gggaacagac ggcctggaac agctttcaga tttgccagcc ccgctttgat agcattaagc
    54481 gttgcgccca cggacatgga gagcgcagcg gctgccagcg cattcgcaat attgtgacgc
    54541 cccggcaacg gcagcagaac atcgacgcta ccggttgggg tttgtagggt aaattccgta
    54601 ccgtgcgagg tcacatggat attggtggcg gtgaaatcgc tgttggcggc attgggtgag
    54661 aaacgccaca ctttgcgtga gccaattacg ctctgccagt tcagccagtc gttgttgtcg
    54721 gcgttcataa tggcgatacc gttttccggc aggccgctaa agatttcacc tttcgctttc
    54781 gcgacacccg caagcgagcc aaaaccttcc agatgcgccg ctgccaggtt gttgaccagc
    54841 gcagcttccg ggcgagtcag actcacagtc caggctattt cgccctgatg gttcgcgcca
    54901 agttcaataa ctgcgtaatc gtattccggc gttaagcgca acaacgtcat cggtacaccg
    54961 atgtcgttgt tgagattgcc tgccgtataa agcgtgttgc cgcactggct taaaatcgcc
    55021 gccgtcatct ctttaacgga ggttttgccg gaggaacctg tcagagcaac cacgcgcgcc
    55081 ggaacttgct ggcgaaccca tgcagccagt tcaccaaacg ccagacgcgt atccttgacg
    55141 attaactgcg gcaggtcgat atccagcgga cggctgacca gtagtgcgcc cgcgccgcca
    55201 gctttcgcct ggtcggcaaa atcatgagca tcgaaacgtt cgcctttcag ggcaacaaac
    55261 aggcagcccg gcgtcagttt tcgggtatca gtggttacag catcaagggt gatatctgca
    55321 ccttgcagtt caccgttgag aatgtcggta agttggctaa gggttacgct aatcatgcaa
    55381 tcacccccag cagacgcgcc accgtgacgc gatcggagta gtccagacgc tgattgccaa
    55441 caatctggta atcttcatgg cctttgcccg cgaccagtac cacatcattc tctttagcct
    55501 gcataacggc gcaagtcacc gcttcagcac ggccttccat cactttggca tgtccggcat
    55561 ctaacattcc cgccagaata tcgttgatga tggcacgcgg ttcttcggta cgcgggttat
    55621 cgtccgtcac caccgccacg tcagcaaact cttcggcaat tgcgcccatc agtggacgct
    55681 tacctttatc gcgatcgcca ccacagccaa agacgcacca cagcttgccc gcacagtgca
    55741 ggcgcgccgc ctgtaaggct ttttccagtg catccggcgt atgcgcgtaa tccaccacca
    55801 ccgtcggttt gcctggcgca gtgaacactt ccatacgtcc gcaaaccggt tgcagacgcg
    55861 cggcggtttt cagcagatca gccagtggat agccgagtgc cagcagtgtc gccagcgcga
    55921 gcagcaggtt gctgacgtta aaagcgccca tcagatggct ttcaatttcg ccatcgcccc
    55981 aacttgagct aaagcgaatc gtcgcaccgc tgtcgtgata gttcacgtcg atcgctttta
    56041 accagcgtcc gtgacagttc ggattaatat gatcttccat tgataccgca accgcgtccg
    56101 gcagttttgc cagccagcgg cggcccactt catcgtcggc gttaataatc gcctgaccgc
    56161 aatgatgttc agaataaagc agccatttcg cagcttcgta gtgttccata tcaccatgat
    56221 aatcaaggtg atcgcggctt aagttggtaa agaccgacgc cgcaaatttc aatgccgcca
    56281 cacggtgctg taccagcccg tgggaggaaa cttccattgc gcaaaacgtc gcgccctgct
    56341 ccaccagccc cgccagctca tgctgaacat cgactgcgga accggtagta ttttctgtcg
    56401 ggatcacttt cccaagcaga ccgttaccaa cggtgcccat taccgcgctg gtttcgccaa
    56461 gcagttggct ccactgcgcc agcagctggg tagtcgtggt tttgccgttg gtgcccgtta
    56521 cgcctacgag acgtaaattg tcagagggtt catggtaaaa gcggcccgcc agtgcagata
    56581 aacgctcatt gagctggctg agatagatga ccggtacgcc gtgcatttca cggatttcac
    56641 cgtcggtcgc ctcatctttc gctttcgcct ctgcaataat ggcagccaca ccttgcgcta
    56701 tcgcctgcgg gatatatcga cgcccgtccg cctgatgacc tactacagct acaaagagat
    56761 cgcccgccgc agccacacgg ctgtcgagtg tcatctctcg cagtgctcgc gaaggtgcgt
    56821 ctggcaccca cggagcaaga aggtcgcgca aattacgatc tgccacctgt cccctcgcct
    56881 tgattaatca caaattcatt tttatcgccc gttgtcagcg catccggctc gatgttcatg
    56941 gtacgcaata cgccgcccat gatggcacca aagaccggcg cggaaacggc gccgccgtag
    57001 tatttacccg cctgcggatc gttgataaca acaaccagcg cgaagcgcgg ctgactcgca
    57061 ggcgcaacgc ctgcggtata agcaatatat ttattgatgt agcgaccgtc cggcccgacc
    57121 tttttcgcgg taccggtttt aatggcgata cgatagcctt taatcgccgc cttcacgccg
    57181 ccgccgcctg gtagcgccac gctttccatc atatgcacca cagtgcggac aatggatccc
    57241 gggaagacac gttcaccggg aaccgggggg tcaactttgg taatcgacag tgggcgataa
    57301 atgccgtagc tgccgatagt tgcgtagact cgcgctaact gtaacggtgt taccattagc
    57361 ccgtagccga aagagaaggt ggccctctct atgtcagacc accgttgttt ttgaggatat
    57421 aagccactgc gttctccgac caaccccaaa ttggtcgctt ttcccagtcc aaaacgtgag
    57481 taagtatcta ctaacgctga ggacggcatc gctaacgcca gcttggaaac accgacgtta
    57541 ctcgacttct gtaatacccc ggtcagggtt aattcgctgt agcgtgccac gtctttgatt
    57601 tcgtggccgt taattcgata aggaatggta ttgagtaccg agttttcccg caccacgcca
    57661 cgttgcaacg cggtcattac caccatcggt ttaaccgttg agcccggttc aaacacgtcg
    57721 gtgatggtac ggttacgcat cgcctctttc ggcgtgccgc tcagattgtt agggttgtat
    57781 gacgggctgt tagccatcgc cagcacttca ccggtgttga catccaccag cacggcgcta
    57841 ccagattcag ccttgttaaa ggccaccgcg ttgttcagtt cgcgataaac cagcgcctgc
    57901 aggcgttcat caatactcag cgccaggttg tgcgctgcct ggctgtcagt agaagaaata
    57961 tcttcaatta cgcgaccata gcggtcttta cgcacaatgc gctcacccgg ctgcccggta
    58021 agccatttat cgaaactctt ctcaacgccc tcaatccctt gactatcgac gttagtaaag
    58081 ccgatgaggt gagcagtcac ttcgccggac ggatagtaac ggcgagactc ttcacgcaga
    58141 tgaatccccg gcagtttcag ttttttgatg tagtccgcca tgtcagggtt cacctgacgc
    58201 gccagataaa taaagcgccc tttcgggttg gcgttaatgc gggctgaaag ctgatccagc
    58261 ggaatattga gcgcgttagc cagcgccttc cagcggtcac cgacgctgat accgccagcg
    58321 tcatgcactt ctttcgggtc agcccaaatc gcttttaccg gcacgctcac cgctaacggg
    58381 cgaccagaac ggtcagtaat catgccgcgg gaggtggaaa cttgctgaac gcgaagagaa
    58441 cgcatgtcgc cctctttcac cagcatatcc ggggagataa cttgtaacca cgctacgcgt
    58501 ccgagcagaa aagccagcgc caggagaata cagccgcata acaacgcaaa acgccaactg
    58561 ataaagttgg catgttcttc ctgacgtttt ggtttctgcg ttttcgccgc tgctttcatg
    58621 cgtcgcgttt atccttattt ttgcactacg atattttctt gtgacggatc aacatgctgc
    58681 atttgcagct tttccgtggc gatcctttcc acccggctat ggtcgccgag cgcattctct
    58741 tcaaggatca ggttgcgcca ttcaatgtct aaagcatctc gctccagcac cagttgttcg
    58801 cgctgagcgg tcagtaaacg ggtatggtgc gccgtggtta ccacagtcac cgccgtcaaa
    58861 ataatgcaaa tgaacaggca gagtggcagc ttcccaaatc gcaaaagatc gtcaccgata
    58921 acaccaggca atgcatggcg ctcgtggctt cccatcgatc ctttaacttt gcttagagct
    58981 tctgtcactc tgctgatcat gcattcgtcc tctctgcaat acgcagaact gaactacggg
    59041 cacgagggtt ctcagccacc tcttcttcgc ccggcattaa cttgcctagt gctcgcagct
    59101 gacggccacc cagttttttg agctgctctt cagtcatcgg taaccctgcc ggaacttgcg
    59161 gaccgcggct gttttcacgc ataaaacgtt tcacaatacg gtcttccagc gagtggaagc
    59221 tgatgatcga aagccgccca cccggggcca gcacgttgag cgagcttttt agcgcctgct
    59281 ctatctcctc cagttcactg tttacccaaa tgcgcaccgc ctggaaggta cgggtcgcgg
    59341 gatgtttaaa cttatctttc accggcgttg caacagccac gacttccgcc agttctttgg
    59401 tgcgggtcat cggctgttcg cggttacgct cgacaatggc gcgggcaatg cgtttggcaa
    59461 aacgctcttc accataggtt ttcaataccc aagcgatatc ggcttcttct gcggtttgta
    59521 gccattcagc ggctgactgc ccacgggttg ggtccatacg catgtccagc ggaccatcgc
    59581 gcataaagga aaagccgcgc tcagcatcat caagttgcgg tgaagagacg ccaagatcga
    59641 ggagaatgcc gtcgatcttg ccgataagat cgcgctcggc aacgtattcg cccagcgcgg
    59701 agaaaggtcc gtggatgatg gagaagcgcg gatcatcaat agtcttcgca acggcgatag
    59761 cctgcgggtc gcgatcgatc gccagcaaac gcccctcttc gccaagctgc gagaggatca
    59821 gacgtgagtg accaccgcga ccaaaagtcc catcaatgta gatgccatca ggacggatat
    59881 tgaggccatt aacggcttca tccagcagca ccgtagtatg tttatagttt tccatcattt
    59941 tatagagaca agtcctgcag tcgctccgat aagtctccgg tagccaactg ctctgcgtcg
    60001 atatcttcct tgacctgttg atgccaggtt gtttcatccc acagctcaaa cttgttgaac
    60061 tgtccaacca gcatcacttc ttttgtcagc ccggcatgtt gccgcagtac tggcgcgatt
    60121 aacaatcgac ctgcgccatc catctgacat tcgctggcat gacctaacag taggcgctgc
    60181 acacggcgct caaccgggtt catgctcgac agacgcgata atttttgctc gataatttcc
    60241 cattcaggca gggggtaaag cagcaggcac gggtgatgaa tgtcaatggt gcaaaccatt
    60301 tgaccgacag cgttctcaag cagctgttcc cggtaacggg taggcactga taagcgccct
    60361 ttgctgtcga gattgactaa cgttgctccc cggaacatgc cagcctcacc ccttattccc
    60421 actttgcccc acaatttccc actgaaagga gtttacggag cggaggaaaa gcttgtcaag
    60481 ccagtcacaa ggcataccga ggtaaaaaac ccgatgttta cggctaatat cagcgttgtt
    60541 tattttacgt ccaacgtgcg cagcaaatta acgttatgaa tagctgtaag aaaaaatccc
    60601 tcatcgtcat caccgtttta aaatgactca ctattttcca cagcaaatat aaagtgtcag
    60661 tttgcgacgc gagcggcatt ttaggacata tcttccccgg ttaacagtcc ctgttgcgtc
    60721 tgtgtggcgc ttgatgccag cgggtttgcg caaaagcaaa gaaaagtgct tgttaaatcg
    60781 gcaattcatc tctgcatgta acaaaataat gcaaaaacgc gctgtcgata tcacttaatt
    60841 attgtgaatt tcagctggtg agttaattaa cgggcgggtt taaaagaact tatcaataat
    60901 agtcaggaat agtcttattt actttaagga tattgatgtc cattcccgta ctctacg
//
LOCUS       DAZPLI010000006        59893 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZPLI010000006 DAZPLI010000000
VERSION     DAZPLI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59893
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.006"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..1474)
                     /gene="ggt"
                     /locus_tag="ACHUU3_000328"
     CDS             complement(<1..1474)
                     /gene="ggt"
                     /locus_tag="ACHUU3_000328"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR2080507.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGMGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
     gene            1594..2034
                     /locus_tag="ACHUU3_000329"
     CDS             1594..2034
                     /locus_tag="ACHUU3_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR2080508.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(2021..2764)
                     /gene="ugpQ"
                     /locus_tag="ACHUU3_000330"
     CDS             complement(2021..2764)
                     /gene="ugpQ"
                     /locus_tag="ACHUU3_000330"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR2080509.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(2761..3831)
                     /gene="ugpC"
                     /locus_tag="ACHUU3_000331"
     CDS             complement(2761..3831)
                     /gene="ugpC"
                     /locus_tag="ACHUU3_000331"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFR2080510.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            complement(3833..4678)
                     /gene="ugpE"
                     /locus_tag="ACHUU3_000332"
     CDS             complement(3833..4678)
                     /gene="ugpE"
                     /locus_tag="ACHUU3_000332"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR2080511.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(4675..5562)
                     /gene="ugpA"
                     /locus_tag="ACHUU3_000333"
     CDS             complement(4675..5562)
                     /gene="ugpA"
                     /locus_tag="ACHUU3_000333"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR2080512.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(5660..6724)
                     /gene="ugpB"
                     /locus_tag="ACHUU3_000334"
                     /pseudo
     CDS             complement(5660..6724)
                     /gene="ugpB"
                     /locus_tag="ACHUU3_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            complement(7008..7721)
                     /gene="livF"
                     /locus_tag="ACHUU3_000335"
     CDS             complement(7008..7721)
                     /gene="livF"
                     /locus_tag="ACHUU3_000335"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR2080513.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(7723..8490)
                     /gene="livG"
                     /locus_tag="ACHUU3_000336"
     CDS             complement(7723..8490)
                     /gene="livG"
                     /locus_tag="ACHUU3_000336"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR2080514.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(8487..9764)
                     /gene="livM"
                     /locus_tag="ACHUU3_000337"
     CDS             complement(8487..9764)
                     /gene="livM"
                     /locus_tag="ACHUU3_000337"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR2080515.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(9761..10687)
                     /gene="livH"
                     /locus_tag="ACHUU3_000338"
     CDS             complement(9761..10687)
                     /gene="livH"
                     /locus_tag="ACHUU3_000338"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR2080516.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(10735..11844)
                     /gene="livK"
                     /locus_tag="ACHUU3_000339"
     CDS             complement(10735..11844)
                     /gene="livK"
                     /locus_tag="ACHUU3_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR2080517.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            12268..12651
                     /gene="panM"
                     /locus_tag="ACHUU3_000340"
     CDS             12268..12651
                     /gene="panM"
                     /locus_tag="ACHUU3_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR2080518.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWIADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(12850..13953)
                     /gene="livJ"
                     /locus_tag="ACHUU3_000341"
     CDS             complement(12850..13953)
                     /gene="livJ"
                     /locus_tag="ACHUU3_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR2080519.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(14224..15078)
                     /gene="rpoH"
                     /locus_tag="ACHUU3_000342"
     CDS             complement(14224..15078)
                     /gene="rpoH"
                     /locus_tag="ACHUU3_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR2080520.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(15323..16381)
                     /gene="ftsX"
                     /locus_tag="ACHUU3_000343"
     CDS             complement(15323..16381)
                     /gene="ftsX"
                     /locus_tag="ACHUU3_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR2080521.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(16374..17042)
                     /gene="ftsE"
                     /locus_tag="ACHUU3_000344"
     CDS             complement(16374..17042)
                     /gene="ftsE"
                     /locus_tag="ACHUU3_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR2080522.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            complement(17045..18541)
                     /gene="ftsY"
                     /locus_tag="ACHUU3_000345"
     CDS             complement(17045..18541)
                     /gene="ftsY"
                     /locus_tag="ACHUU3_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR2080523.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            18691..19287
                     /gene="rsmD"
                     /locus_tag="ACHUU3_000346"
     CDS             18691..19287
                     /gene="rsmD"
                     /locus_tag="ACHUU3_000346"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR2080524.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            19277..19546
                     /locus_tag="ACHUU3_000347"
     CDS             19277..19546
                     /locus_tag="ACHUU3_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR2080525.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(19549..19908)
                     /locus_tag="ACHUU3_000348"
     CDS             complement(19549..19908)
                     /locus_tag="ACHUU3_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR2080526.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            20049..20675
                     /locus_tag="ACHUU3_000349"
     CDS             20049..20675
                     /locus_tag="ACHUU3_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR2080527.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            20749..22947
                     /gene="zntA"
                     /locus_tag="ACHUU3_000350"
     CDS             20749..22947
                     /gene="zntA"
                     /locus_tag="ACHUU3_000350"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR2080528.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(23049..23294)
                     /gene="tusA"
                     /locus_tag="ACHUU3_000351"
     CDS             complement(23049..23294)
                     /gene="tusA"
                     /locus_tag="ACHUU3_000351"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR2080529.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKEMDGLPYRYLIRKGG"
     gene            23515..24180
                     /locus_tag="ACHUU3_000352"
     CDS             23515..24180
                     /locus_tag="ACHUU3_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR2080530.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            24253..24810
                     /gene="dcrB"
                     /locus_tag="ACHUU3_000353"
     CDS             24253..24810
                     /gene="dcrB"
                     /locus_tag="ACHUU3_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR2080531.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(24814..26031)
                     /locus_tag="ACHUU3_000354"
     CDS             complement(24814..26031)
                     /locus_tag="ACHUU3_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2080532.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            26163..27212
                     /locus_tag="ACHUU3_000355"
     CDS             26163..27212
                     /locus_tag="ACHUU3_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR2080533.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            27267..27854
                     /gene="acpT"
                     /locus_tag="ACHUU3_000356"
     CDS             27267..27854
                     /gene="acpT"
                     /locus_tag="ACHUU3_000356"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR2080534.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            27965..29539
                     /gene="nikA"
                     /locus_tag="ACHUU3_000357"
     CDS             27965..29539
                     /gene="nikA"
                     /locus_tag="ACHUU3_000357"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR2080535.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            29539..30483
                     /gene="nikB"
                     /locus_tag="ACHUU3_000358"
     CDS             29539..30483
                     /gene="nikB"
                     /locus_tag="ACHUU3_000358"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR2080536.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            30480..31313
                     /gene="nikC"
                     /locus_tag="ACHUU3_000359"
     CDS             30480..31313
                     /gene="nikC"
                     /locus_tag="ACHUU3_000359"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR2080537.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            31313..32077
                     /gene="nikD"
                     /locus_tag="ACHUU3_000360"
     CDS             31313..32077
                     /gene="nikD"
                     /locus_tag="ACHUU3_000360"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR2080538.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            32074..32880
                     /gene="nikE"
                     /locus_tag="ACHUU3_000361"
     CDS             32074..32880
                     /gene="nikE"
                     /locus_tag="ACHUU3_000361"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR2080539.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            32886..33287
                     /gene="nikR"
                     /locus_tag="ACHUU3_000362"
     CDS             32886..33287
                     /gene="nikR"
                     /locus_tag="ACHUU3_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR2080540.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(33407..33766)
                     /locus_tag="ACHUU3_000363"
     CDS             complement(33407..33766)
                     /locus_tag="ACHUU3_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR2080541.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(33763..34034)
                     /locus_tag="ACHUU3_000364"
                     /pseudo
     CDS             complement(33763..34034)
                     /locus_tag="ACHUU3_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            complement(34107..35231)
                     /locus_tag="ACHUU3_000365"
     CDS             complement(34107..35231)
                     /locus_tag="ACHUU3_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR2080542.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(35231..37966)
                     /gene="rbbA"
                     /locus_tag="ACHUU3_000366"
     CDS             complement(35231..37966)
                     /gene="rbbA"
                     /locus_tag="ACHUU3_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR2080543.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(37963..39030)
                     /locus_tag="ACHUU3_000367"
     CDS             complement(37963..39030)
                     /locus_tag="ACHUU3_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR2080544.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(39093..39206)
                     /gene="yhiY"
                     /locus_tag="ACHUU3_000368"
     CDS             complement(39093..39206)
                     /gene="yhiY"
                     /locus_tag="ACHUU3_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR2080545.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            complement(39396..41018)
                     /locus_tag="ACHUU3_000369"
     CDS             complement(39396..41018)
                     /locus_tag="ACHUU3_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR2080546.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTINDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(41280..42887)
                     /locus_tag="ACHUU3_000370"
     CDS             complement(41280..42887)
                     /locus_tag="ACHUU3_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR2080547.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKSDITYKVNGLS
                     IIPENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNAN
                     TAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYN
                     LLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMDG
                     NKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKVL
                     TSHHGIIRNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRS
                     NAVASTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTM
                     GLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            43272..44324
                     /locus_tag="ACHUU3_000371"
     CDS             43272..44324
                     /locus_tag="ACHUU3_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFR2080548.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(44640..45842)
                     /locus_tag="ACHUU3_000372"
     CDS             complement(44640..45842)
                     /locus_tag="ACHUU3_000372"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR2080549.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            46074..47573
                     /gene="pitA"
                     /locus_tag="ACHUU3_000373"
     CDS             46074..47573
                     /gene="pitA"
                     /locus_tag="ACHUU3_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR2080550.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(47817..48152)
                     /gene="uspB"
                     /locus_tag="ACHUU3_000374"
     CDS             complement(47817..48152)
                     /gene="uspB"
                     /locus_tag="ACHUU3_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR2080551.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            48543..48977
                     /gene="uspA"
                     /locus_tag="ACHUU3_000375"
     CDS             48543..48977
                     /gene="uspA"
                     /locus_tag="ACHUU3_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR2080552.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            complement(49076..49129)
                     /locus_tag="ACHUU3_000376"
     CDS             complement(49076..49129)
                     /locus_tag="ACHUU3_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2080553.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            49295..50764
                     /gene="dtpB"
                     /locus_tag="ACHUU3_000377"
     CDS             49295..50764
                     /gene="dtpB"
                     /locus_tag="ACHUU3_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFR2080554.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(50813..51565)
                     /gene="rsmJ"
                     /locus_tag="ACHUU3_000378"
     CDS             complement(50813..51565)
                     /gene="rsmJ"
                     /locus_tag="ACHUU3_000378"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR2080555.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(51573..53615)
                     /gene="prlC"
                     /locus_tag="ACHUU3_000379"
     CDS             complement(51573..53615)
                     /gene="prlC"
                     /locus_tag="ACHUU3_000379"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR2080556.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            53818..54660
                     /gene="rlmJ"
                     /locus_tag="ACHUU3_000380"
     CDS             53818..54660
                     /gene="rlmJ"
                     /locus_tag="ACHUU3_000380"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR2080557.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            54732..56084
                     /gene="gorA"
                     /locus_tag="ACHUU3_000381"
     CDS             54732..56084
                     /gene="gorA"
                     /locus_tag="ACHUU3_000381"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR2080558.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(56138..56221)
                     /gene="dinQ"
                     /locus_tag="ACHUU3_000382"
     CDS             complement(56138..56221)
                     /gene="dinQ"
                     /locus_tag="ACHUU3_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR2080559.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            56249..56422
                     /locus_tag="ACHUU3_000383"
     CDS             56249..56422
                     /locus_tag="ACHUU3_000383"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2080560.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            56961..57314
                     /gene="arsR"
                     /locus_tag="ACHUU3_000384"
     CDS             56961..57314
                     /gene="arsR"
                     /locus_tag="ACHUU3_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR2080561.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            57368..58657
                     /gene="arsB"
                     /locus_tag="ACHUU3_000385"
     CDS             57368..58657
                     /gene="arsB"
                     /locus_tag="ACHUU3_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR2080562.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            58670..59095
                     /gene="arsC"
                     /locus_tag="ACHUU3_000386"
     CDS             58670..59095
                     /gene="arsC"
                     /locus_tag="ACHUU3_000386"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR2080563.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(59229..59525)
                     /locus_tag="ACHUU3_000387"
     CDS             complement(59229..59525)
                     /locus_tag="ACHUU3_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR2080564.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            59632..>59893
                     /locus_tag="ACHUU3_000388"
     CDS             59632..>59893
                     /locus_tag="ACHUU3_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR2080565.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
BASE COUNT        14009 a        16146 c        15122 g        14616 t
ORIGIN      
        1 gcacggtggt aataatccga ctcccgccag gactaccggt aaccagccaa gttttaccgt
       61 ctttcaccac aatggttggt gacatcgatg acagcggacg tttattcggc ccgacggcgt
      121 tggcatcacc gcccaccagc ccgtaaatgt tcggtacgcc cggtttggcg gagaaatcat
      181 ccatctgatt attaagcaga ataccgctct cgcccgcgac aatgcccata ccgaaggtgg
      241 tgttcagcgt ataggtcacc gccaccgcgt taccgtcttt atccactaca gagtaatggg
      301 tagtttgatt actctcataa ggcgcaagct tgccagggcg aatctcactg gacggcttcg
      361 ctttgttgat atcaatttga tcggcaatgg atttggcata ggctttattg gtcagtgcct
      421 gccacgggac tttgacaaaa tccgggtcgc caagatattc cgagcggtcg gcgtaggcgt
      481 atttctccgc ttctgccatg atttgcatcg catcggcgct gccaaagccg tatttcttca
      541 tatcgaagtt ttccagaata ttgaggattt gtacgatatg gatcccgccg gaggatggcg
      601 gtggcatgga gtaaacctga tacccgcgat aatcgccgct tatcggagtg cgttcgaccg
      661 ctttataggc cgctaaatct tctttagtga tcaagccacc gtttttctgc atctcctggg
      721 cgatctgttc cgcaatcgtg cctttataga attcgtccgg gccgttttca gcaatcatct
      781 ccaggctctt tgccaggttc gcctgcacca gcgtgtcgcc ctttttcagc ggctcgccct
      841 ctttccagaa gatagcttta ctgttttcgt gattcggcaa cacttcgcta ccgtaggttt
      901 tgagatcgtc agccagcgcg tcgttaacga taaaaccatc gcgtgccagt ttaaacgcgg
      961 gctgcacgac tttgttcagc ggcatggtgc cgtatttatc cagcgccagc gagaaacctg
     1021 ctaccgtacc cggtgtgccg gaagccagat gcgaagtgag tgattttttg ctgtccgggt
     1081 tgccctgatc atcgaggaac atatcgcggg tcgctttggc gggtgccatt tcgcggaaat
     1141 cgatagccgt ggtattgcca tttttcgagc ggattaacat aaaaccacca ccgcccagat
     1201 tccctgcctg cggatgcgtt accgccagcg cgtagcccac cgccacggcg gcatcaacgg
     1261 cattcccgcc ctccttgagg atatccaccc ccacctgagt ggcagtggcg tccacagacg
     1321 ctaccattcc ctgtttcgcg cgtaccgggt ggaagacatc ttcctccaca ccatacgaga
     1381 cgggcggcgc aggaggcgcg gcggcggcgc taaaacaact tcctgagagc agagcagcaa
     1441 tggccacccg gcgtaaaaac gtcggtttta tcatcgttat tctccagaga ttaaggggca
     1501 accccagcta agcctggtat ataactctga attaatcatc gttttgtcgg gaagcgagta
     1561 aacttaaagg atatcttcag aggaggatac gcgatgaaac gacttctgat tcttacggca
     1621 ctcctgccgt ttgtcggctt tgcacagccc attaatactc tgaacaaccc taaccagccg
     1681 gggtatcaga tacccagcca gcagcggatg caaacccaga tgcagactca gcaaatccag
     1741 caaaaaggga tgctgaatca gcaactgaaa acgcaaactc agttgcaaca gcagcattta
     1801 gaaaaccaga taaacaataa ttctcagcgg gtgttgcagt cacagccggg ggagcgaaat
     1861 cccgcccggc agcaaatgct gcccaacacc aacggcggga tgttaaacag caaccgtaat
     1921 ccggatagtt cgttgaatca gcagcatatg ctgccggaaa ggagaaacgg cgacatgctg
     1981 aatcagccca gcacgccgca gcctgacatt ccgttgaaaa ctattgggcc gtaaagttcg
     2041 gaccaatcac gtcaatcgca tcggtacaga tgcaatccac gccccagcgc agcaactctg
     2101 ccgcgcgctg gggtttattg acggtataaa ccagaatccg taatccggcg tctttcaact
     2161 gcatcactcg cgctttatcg agtaacttat gattgagatg aatagagacg cagcccagcc
     2221 gcgcggtcag ttcgcgccag tcgtcgcgcc actcatcaag caataaacca cgcggcagtt
     2281 ccggtgccgc ctgttgtgca gcttctaaag catcaatctc aaacgatgac agcagcggcg
     2341 gcgtcatacc ggcccacagt tggcgtgccg ccagcgccac cattttgccc gttaatggtc
     2401 cagtgccagt ggtgggtttg atttcgatat tcgccatcat cccgtgttcg cgacagcgtt
     2461 ccgccacctg cgaaagcaac ggcagcggct cacctttaaa cgctttgctg taccaactgc
     2521 ccgcatccac gcgcagtaaa tcctgccagt tcagttcacc cgcgacgccc cagccgttgc
     2581 tggtgcgttc gagattgtcg tcatggagca ggaagatctc gccatctttc gataacttcg
     2641 cgtcaaattc gatcatctta tgaccgtatt ttgccccgac gtcgattgcc gccagggtgt
     2701 tttccggggc cagcttaccg ccgccacgat gagcgacgat gcggggataa ggccagttac
     2761 tcatactcgt tgtcctgttt caccatcaaa aagatgcagc tgattttccg ccaaatgcag
     2821 ccacagcgtg ctgcctgccg tcgggcgctc ctgatgcgcc agtcgcacca ccagcttctg
     2881 ctcgccccag cgtccgtgcg ccaggttatc tgcgccgagg atctccagcg tgtccatcac
     2941 cagcggcaca ccaccttctg cctgcgagct tagcgctata tgttccgggc gaatgccgag
     3001 agtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca cgataccgcc
     3061 gtccagttcg aaatgcgtgc cttcattatt cacgcggcct gtcagcaggt tcatcgccgg
     3121 gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt caactggtgt
     3181 gccaatctgt tcggcaacgc caccgttcat caccattact cgctgggcga gcgtcatcgc
     3241 ttcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt gcaactgttg
     3301 cagttcaaga cgcatctgca cgcgcagctt ggcatcgagg ttagagagcg gctcatcaaa
     3361 caagaacacc gccggatcgc gcacaatcgc gcggcccatc gccacacgct ggcgctgacc
     3421 gccggatagc tcacgcggac gacgcttaag cagaccgtcc agttccagaa tgcgcgccgc
     3481 ttctttaacg cgctcggcaa tttgctgctt gcccatgccg cgaattttca gcccccacgc
     3541 catgttctcc tctacgctca tatgcggata aagcgcgtaa ttctggaaca ccatcgcaat
     3601 cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga tatcgccttc
     3661 tgtcacccgc tccagcccgg caaccatgcg cagcagcgtc gatttcccgc agccagacgg
     3721 cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt taatgacctg
     3781 ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca tcttatttct
     3841 cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc accaccggag
     3901 ggataagcgt taacaacatc gccgccatta ccgagttcca ttccgtggtg ccttcgcctg
     3961 tagcgatcat ccctttgatc cctgccacgg tggtgccgag atcaacgtcg gtaataatca
     4021 acaacggcca caaatactga ttccagccgt agataaaggt gatcacaaac agcgccgcca
     4081 gattagtttt ggacaacgga aaaacgatgt cgcaaaagaa acgcattggt gacgcgccgt
     4141 cgatccgcgc ggcttccacc aattcatccg gtagggtcat aaagaactgg cggaacagaa
     4201 acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg tcgagcattt
     4261 tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc agcatcaggg
     4321 tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag acaatggcaa
     4381 atgccgagag catcgagacg gtaattttgc cgagcgtaat gctgaacgcc atcacaaagc
     4441 tgttaagcaa catccgccag aacggcgcgc tattcgtgcc taccccgtta acccagatgt
     4501 tgtggatatt ttccagcaga tgtgtgccgg ggatgagcgt catcggcgcg gcatagacgg
     4561 cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg atcaccgcga
     4621 tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc tcaatcattg
     4681 gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga tgacgaggaa
     4741 catcaacacc accgactgcg cggcagacga agccagatcc agtccggtaa agccttcgcg
     4801 gtagatctta taaatcagcg tagtggtggc ctgaaccggc ccgccggacg tggcggcgtc
     4861 gatcaccggg aaggtgtcga agaaggcgta caccaggttc accaccagca ggaagaaact
     4921 caccggagcg ataagcggca gagcaatctt aaagaagcgg cgaatcggcc ctgcgccgtc
     4981 aatagctgcg gcttcaatca acgaacgggg aatggagtgc agcgcggcat agaagaacag
     5041 aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca ttgcctgacc
     5101 gctgttttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg tgatcagccc
     5161 gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag ccacggcata
     5221 cggcagcagc atcaaggttt gatagaaacg gctgccgcgc acgatgtact ccaccagcgc
     5281 cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga attttatcgt
     5341 cgtccagaag gagtctagat agtagctgtc atggaacagc gtgacgaagt tatccagacc
     5401 gacaaactgg ctggagaaac caaacggatc gacgctttgc cgcgagtacc acaacgcttc
     5461 gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga ccagcagata
     5521 aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta attcctgaac
     5581 tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga taagcgaaac
     5641 gcatccgaca ttacactgat taagacttcg tcgatttctc aaagcggcgc agcaactgat
     5701 ttccacgctc aacggcggta tccagtgcct gctgtggtgt cttcttaccg gtccacacgc
     5761 tctccagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga cgcagccctt
     5821 tggtgaacgg caacggcggc ttattcagca tctgacgcgt cgcggtatcc gcccctgggt
     5881 ttttctcata aaagccctgc tcacgggtca ggtcatacgc tgctttggtg attggcagat
     5941 aaccggtttt ctgatgccac tcggcagcgt tttctggctt cgcgaggaag tcgaggaact
     6001 tcgccacgcc ggtatacgtt tctttatttt tgccctgcat cacccacagg ctggccccgc
     6061 cgataatggc gttttgcggt gcatctttcg catcggcgtc gtaaggcatc atgcctacgc
     6121 catagttaaa tttggcgtac tcacgaatat tagcgagaga accggaagag gcggtggtca
     6181 tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg acgtagctga
     6241 agtcgccctt cttgttcatc tcttcgagca tggcaatgtg tttcacctgc tccggcttgt
     6301 tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca aacggcagac
     6361 cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg gcgtagccgc
     6421 acttcatacc ggaggctttc agttttgcgg aagagtccgc cagatcctgc caggttttcg
     6481 gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa tagagaacgg
     6541 gagtcgagct gttgaatggc tgggagagta agtggcccgt tttgctgtcg gagtagtaac
     6601 cggaaaccgt cggcacaaac tgcgactcat cgaattgaat ccctgcctct ttaaacacgt
     6661 catataccgg tttaatggct ttcgatgcca tcatggtggc ggtgccaact tcataaacct
     6721 gcaagtaagg tgtcagaaag atgacaaggc ggtgacggcg tgggtgaggg aaaatgggag
     6781 atggggcacg gataagcgga aaaatagaag gtctgaatca aactctacag attactcatc
     6841 gtttcatgcc ggatgcggcg taaacgcctt atccggccta caagatcgtg caaattcaat
     6901 atattgcaac tcacccagta ggcctgataa gcgcagcgca tcaggcaatt ttacatttgt
     6961 cacctgtctc aaaggagtct tttgactcct tatcaatcaa cgtgttatta cccgcctaaa
     7021 tacgcacttc tcaccgcttc attcgccagt agtgcatcac cagtatcgga gagcaccaca
     7081 tggccgtttt ccagcacgta gccgcgatcc gccagcttta gcgcctggtt ggcgttctgc
     7141 tcgacgagga agatagtcat cccctgctcg cgcagctgct cgatggtgtc gaaaatttgc
     7201 tggatgataa tcggcgcaag accaagcgat ggctcatcaa gtagcagcaa acgcgggttg
     7261 ctcatcagcg cgcgaccaat cgccagcatt tgttgctcac cgccggacat ggtgcctgcc
     7321 cgctgaacac ggcgctcatg cagacgtggg aacagttcat acacccactt tatgcgctcc
     7381 tggaactggt cgcgttcggc aaaaaagccg cccatcgcca ggttctcttc caccgtcatc
     7441 cgcgagaaga cgcgacgccc ttccgggaca atcgctaccg cttcgcgcat gattttcgcc
     7501 gtctgccagt cggtaatgtc tttatcatca aacacaattc gcccgctggt agcgcgcgga
     7561 tcgccgcata acgtgccgag caaggtggtt ttccccgccc cgttcgcgcc aatgagggtg
     7621 acaatctcgc cctgattgat atgcaggctc acctcatgca gcgcctggat tttgccgtag
     7681 tgggcgctga ctttgtcaaa ggacaacatg actttttcca tcttatgcct cacctaaata
     7741 ggcacggatc acgtccgggt tattacggat ttgctccggc gtaccgtttg ccagtggcgt
     7801 cccctgattg accacataaa ttcggtctga aatccccatc accagcttca tatcgtgttc
     7861 aatcaacaag atagtggtgt tgtgatgatt gcgcagttcg gcaatcagct catccagctc
     7921 tttggtttct tttgggttaa gacctgccgc cggttcgtcg agcattaaaa tctccggctg
     7981 cgtcaccatg cagcgggcaa tctccagacg gcgttggtca ccataggcca ggttactcgc
     8041 ctgacggttg gcgtgttcca gcaaaccaat gcgctcaagc caggtggcgg cgcggtcgag
     8101 cgcttcgctc tgggcgcgac ggaaagaagg cgttttcaac aggccagaga acagcccggt
     8161 tttcagttgt tgatgctgcg ccaccagcag gttttcaatt accgtcattt cacggaacag
     8221 acgcacatgc tggaaggtgc gcaccacgcc catgcgggca atttgctgcc ccggtaaacc
     8281 ttccaggtgc tgattgcgca gtaaaatggt gccgccggtg ggtttgtaga atccggtcag
     8341 acagttaaaa accgtagttt ttccggcacc gttagggccg attaacgaga cgatctcctg
     8401 cgggtacagt tcaagattga cgttgttcac cgccagcagg ccgccgaagc gcatcatcag
     8461 gccgttcaca gataataatg gctgactcat gcctgctctc ctttcgctgc gccgtttttc
     8521 agcttcagtt gcgggcgcgt catgggcagc aagccctgcg gacgccagat catcatcagc
     8581 accatcaaac caccgagcat taacatgctg tattcgttga aatcacgcat caattcgcgc
     8641 gataccacca gcaaaattgc cgccagaatc accgcaaact gcgatcccat accgccgagc
     8701 accactatcg ccagtacaaa cgccgattca gcaaaggtga aggattccgg gctgacaaag
     8761 ccctggcgcg cagcaaacag cgttccggca aaaccggcaa acgcggcact gatggtaaag
     8821 gcagtcagct tgatacgacg cgggcttaag cccagcgaac ggcaggcgat ctcatcttca
     8881 cgcaacgctt cccacgcacg ccccagcggc atccgcagca ggcggttaat gacaaacagg
     8941 cttagcacca ccagcagcaa cgccaccaga tacaggaaga tgacgcgatc ggagggatcg
     9001 tatttcaggc caaagaaatt actgaacgtg tcccaaccgc cttcacgggc ggtacggctg
     9061 aactcaaggc cgaagaaagt cggtttcggg atctgactga taccgttcgg gccgccggta
     9121 atttcggtgt tattgagcag caatatgcgc acaatttcgc cgaaaccgag ggtaacgatc
     9181 gccagataat caccgcgcag acgcaacacc ggaaaaccaa gcaggaagcc cgccgccgct
     9241 gccatcagtc cggcaatcgg caaacaggtc cagaacccca gaccgtaata gtgattgagc
     9301 agcgcaaaag tgtaagcgcc gatggcgtaa aaaccgccgt accccagcac cagcagacca
     9361 gaaagaccaa caaccacatt cagcccgaga ccgaggataa tgtagatcat ggtcagagtg
     9421 gcgatatcca ccgtcccacg tgacaccata aacggccacg acaccgcaag caccagcagc
     9481 gccacgagga acagtttctg cttcaccgtg gagccatcaa tcgcgggcag aataaacttc
     9541 ggcccggaaa cgctttttag ccctttctgg aaagcaggtc gcaaaagctg gaagaaaaag
     9601 accaccgccg tgccgataaa cacccactgc caacggacat ccgaagcgct gtcgaccacc
     9661 agtttggtgc catccagctc cagttgcacg cccataaaga cgccagcgag gacaaagaac
     9721 atcgcggcag agagcagtgc cattgcaata tgcatcggtt tcatactttc tctacctccg
     9781 ggcgacccag aataccggtc ggcatcacca gcagcaccag aatcagcagg gcgaatgaga
     9841 ccacatcttt atattccgta ctcagatagg cagaagagag cgcctccgca atccccagga
     9901 tcagcccgcc aatcatcgcc cctggaatgc tgccaatccc accgagcacc gcagcggtaa
     9961 aggctttcat cccggccata aagccgatat aggggttaat gacgccgtag aactgaccga
    10021 gcagtacgcc agccactgcc gccatcgccg cgccaataac aaaggtcaaa gcaatcaccc
    10081 ggtcggtgtt gatgccaagc aggctcgcca ttttcagatc ttccgcgcag gcacggcagg
    10141 cgcgccccat acgggaataa cgaatgaaaa tcgtcagcgc cagcatggcg aggaaagtaa
    10201 caatccagat cactgcctgc atggtggtaa tagaggcaga gaagttttcg ctatgcccca
    10261 ccacccactg accgttaaac agactgggca gcgccacgtc acgcgaacct tcggtcaggc
    10321 taacgtagtt ttgcaggaag atggacatgc cgattgccga gatgagcgca atcaggcgct
    10381 tagagttacg caccgggcga taggctaccc gttcgatact ccagccgtag gcgctggcaa
    10441 tgacgattgc gccgacgaat cccgccgcca ccagcagcca gccggtatca atgcccatca
    10501 tcatcagtgc ggcgatgatc ataaaggaga catagctacc aatcatgtaa acctcgccgt
    10561 gggcgaagtt gatcatgccg ataatgccgt aaaccatggt gtagccgatg gctatcagcg
    10621 cgtaggtact gcccagcgtg acgccgttaa acatctgctg caagaaatat aaaaactgct
    10681 cagacataag gtaacctttc taaacccgcc cgcatttcac gggcggtggg atgatcactt
    10741 ggctgccgtg gatgaaccat cggcgtgcca ctggaagaca ccaaaatcaa atcccttaag
    10801 atcgcctttt tcatcccagt tcagcggccc aatcacggtg tttgcaccgt tagcttttaa
    10861 atctttcacc agcgccagcg gctcatcgct gccagtacgc tcaagggcag tcgccagaga
    10921 ttgcaccgcc gcgtaggtga tccagacata aggcccggac ggatctttct tgtctgcttt
    10981 cagcgcatca acgatgccct ggtttgccgg atcctggtca tagcgttttg gcatagtgac
    11041 caacatgcct tcggcagcat cgccagcaat attcgacaat gatgcgttac ccacaccttc
    11101 cggccccata aactgagttt tcaggccaac ggaacgggcc tggcgcagca tctgccccat
    11161 ttccgggtag taaccgccgt agtaaacgaa gtcgatgttt tcttttttca ggcgggcgat
    11221 cagcgcggag aaatctttct ccccggcggt aataccgtcg aagaagacga cgttggcgtt
    11281 agccgctttc agcccgtcct gcaccgaacg cgccagccct tcgccatact gttgtttgtc
    11341 gtgaatgatg gcgatgcgct ggggcttcac cgtctcaaga atgtattttg ccgccgttgg
    11401 cccctgggaa gagtccagcc cggcagtacg cataatgtgt tgataaccgc gttgggtcag
    11461 ctccgggttg gtcgctcccg gcgagatcat cagaatacct tcgtcttcgt agatatctga
    11521 tgcaggttgg gtagaagaag aacacagatg accaataacg tatttaatgc cgtcattaac
    11581 gattttgttg gcgaccgcaa cggcttgttt cgggtcgcag gcgtcgtcat attccacgcc
    11641 aaccagcttg tcgcccttaa ttcccccttt ggcattaatg tctttaatcg cctgacgcgc
    11701 gccgttaaat tccatatcgc cccactgggc aatcgggccg gacatcgcgc cgacaacggc
    11761 gactttaata tcgtcagcca tagcggtgtg tgaaattgcc agtgcaatca tccctgcgat
    11821 gatagttttc gcattccgtt tcatagtcaa aaatccccat tcgtgatgtt gtgttgcttt
    11881 gtttttatgt gttaacaaat cagactgttc tttttttata ctgcactgtt tttgcctgtc
    11941 tgattaaggg tttagcgcag tattttgtga taacagtgat taaaatccct atttttcagt
    12001 cgattaagaa cagataatat tctgaattta ttgatagata aacagaaaaa agtgcctttg
    12061 tcagcataaa ataacggcac aaagggcgga ataattcact accattcagg ggattatgct
    12121 gggtattttt cattctctaa tgttttaact ttgtaattat tgctgttaaa aaattaatca
    12181 cctgccaaaa gaaataaaaa agagaaagcc tccgattaaa ttatttcgct acactggttc
    12241 cacttttgtg atttacacgg gttacccatg aagctgacca tcattcgatt agaaaacttt
    12301 agcgaccagg accggattga cctgcaaaag atctggccgg agtattcccc ttcctcttta
    12361 caggttgacg ataaccaccg tatctacgcc gcgcgtttta acgagcgcct gctcgctgcc
    12421 gtacgggtaa ccttaagcgg caccgaggga gcactggatt ccctgcgtgt gcgggaagtc
    12481 acccgacgtc gcggtgtggg gcaatatctg ctggaagagg ttttgcgtaa caatcctggc
    12541 gtttcatgct ggtggatagc ggatgcaggc gtggaagatc gcggtgtgat gacagcgttt
    12601 atgcaggcac tgggatttac ggcgcaacag ggcggctggg agaagcgtta atcgtcaagt
    12661 tgatttcaaa agtgatgacc aacgagaaat tgcctgatgc gctacgctta tcaggcctac
    12721 aatgtgtgtt gcaatttact gatttctttg gatcttatag gccggataag gcgtttacgc
    12781 cgcatccggc atgaagcaac gtactcgata ttagcaattt ggcggcaacc caaagttgcc
    12841 gattaatgat tacttcgcat ctgtcgccgt gccgttggcg tgccagtcaa atacgccgaa
    12901 ctcaaagcct ttcagatcgc ctttctcatc ccacgtcagc ggtcccatta cggtatccac
    12961 ggagttcgct ttcaggtatt tggcgatttc agccggatcg tcagactgat tcaggcccgc
    13021 ctgcaaagat tgcagcgcgg cgtaggtggt ccaaacgaat gcgccgcttg ggtcctgttt
    13081 tttcgctttg atcgcgtcaa caatgggttt gtttgccgga acctgatcgt agttcttcgg
    13141 cttggtcacc agcagccctt ccgctgattc gcccgcaatg ttagacagcg aaacgttagc
    13201 cacaccttcc ggccccataa actgagtttt cagccctgcc gcgcgtgcct gacgcaggat
    13261 ttgccccatt tccgggtggt aaccgccgta gtaaacgaag tcgatattct ctttcttcag
    13321 acgcgccacc agcgtagaga aatctttttc cccggcggtg ataccatcaa agaacaccac
    13381 gttagcattg cctttcttca ggccgtcctg caccgctcgt gccagacctt cgccgtattg
    13441 ctgtttgtcg tgaacgatag caatacgctg cggtttcact ttctcaagaa tatatttcgc
    13501 cgccgtcggc ccctggtcgg agtccaggcc ggtggtgcgc aggatcagct gatagccacg
    13561 ggcggtcagc tccggcgcgg ttgccgctgg ggtgatcatt aaaatgcctt cgtcttcgta
    13621 gatgtcagac gcaggctgcg ttgatgaaga acagaggtga ccaatcacat atttaatgcc
    13681 gtcgttaacg actttgttcg ccaccgcaac cgcctgtttc gggtcacagg catcgtcata
    13741 ttttacgatt tgcagtttgt tgcctttaat gccgccttta gcgttgatat ccgcaaccgc
    13801 ctgctctgcg ccggtaaact cttggtcacc gtactgcgca accggaccgg acattgcgcc
    13861 cacgaccgcg actttaatat cttctgccag agccatattg ctgaatgcca gcgcgataca
    13921 tcctgccagt aacgctttac cctttatgtt catcctgaga atccccattc ttctggttat
    13981 tacgtgtgtt gtgatgttgt tgttcagcac tttatttcgt tttatgcatg gctacccgtg
    14041 ctttagcagc atactctgct aaaacatacc cgatttttat gatattggaa tagctatttt
    14101 gacagtttat taacaatctg cgtggggatt ggcgttttgc cggaggggaa attgattatt
    14161 gtagaggccc aaaaaacaaa aaccccggac tcgcatccag ggttctctgc ttaatagcgg
    14221 aaattacgct tctatagcag cgcgcagttt tttcatcgcg ttcttttcca gctggcgtac
    14281 acgttcagcg gaaacaccgt aacggtcagc cagttcctgc aacgtggact tgttgtcttc
    14341 gtccagccag cgcgcgcgga tgatgtcctg gctgcgttcg tccagacctt gcatcgcgtc
    14401 ggtcagacgg tttgccgcct gctcttccca gttatcatct tcgatgccgt cggcaaagtt
    14461 agatgattta tcctgcagat agagcaccgg tgccatcggc tggctgtcgg aatcgtcgtc
    14521 ggaagacagg tcaaaggtca tgtcctgtgc cgccatacgt gattccatct cacgaacgtc
    14581 tttgctggtt acgcccagtt cacgggccac catttcgact tcatcctggt taaaccagcc
    14641 cagacgctgc ttggttttac gcaggttgaa gaacagtttg cgctgcgctt tggtggtcgc
    14701 aactttgacg atacgccagt tacgcagaac gtattcgtgg atctctgctt tgatccagtg
    14761 aacggcgaag gagaccaggc gcacacccac ttccgggttg aaacggcgca ctgctttcat
    14821 caggccgatg ttaccttcct gaatcaaatc cgcctgtggc aggccatagc ccgcataatt
    14881 acgagcaata tgaacaacaa accgcaggtg agacaggatc agcgttttag ctgcttccag
    14941 atcgccatgg taatgcagct tttcagccag cgcccgctcc tcgtcagccg acaacatcgg
    15001 ccacgcgtta gctgcccgga tgtaggaatc caggttgcca actggggcta aagctaaact
    15061 ttgcattttg tcagtcattc aaatcctctc aatcgatatc ttctggcgct tcagtggtag
    15121 caacaactgt gccagagctt aagagcaacg aggttatcat ccactgtttt atcagacagt
    15181 gattttatcc acaagttcaa tgcaagcttg tgaataaatt acgcacaaaa tgtgacatag
    15241 agatgaaata ccgggaagag acaacgggat ctctttccct gctacggaac ccattgcagg
    15301 gaaagagtat aacacgcttt tattattcag gcgtaaagtg gcgtaaatgt tgtaccgtgg
    15361 caagccacgc tgccacccag ccaatcatcg agcataccag cagcaatagc aggcattcat
    15421 cgaacgataa accattgata tcaaacttcg ttccgaaaac ctgtgccact tccgcaaccg
    15481 ccgatgacaa tcgcagcacc agaatttctg acaaaattaa tgacaacaat gcgccagaaa
    15541 atcccagcag tgcgccacca tacaggaacg ggcgaaggat gaaaccatcc gtcgcaccaa
    15601 tcagtttctg cacgttgatg gagtcacggc gagcaaagat gctcagacgt acgctgttgc
    15661 cgatgacgag gaacactgcc gccaccatca acacgccgat catcgccgaa acgcgcccga
    15721 ccagcccggt caacgccgcc agacgggcaa accagctgtc atccatccgc acttcgtcaa
    15781 tgccgttaat ctgcgtgata cgatcacgca gcgtattgag tgattccgtc ccctgaaaat
    15841 cgagtttcgg gatcaccacc gccactgccg gaagcgggtt ttcttccagc atatccagcg
    15901 caccaccaaa accagaccag ttacggaact cgcccagtgc gtcttcacga gaaagatagt
    15961 tcactttctc cacgccttgc tcggcctgca actgtgccac cacgcccgca gcggcgtcat
    16021 cgtccagcgt tttttgcaag taaacagtga tttgcggtga cggataatac tgcgtcgccg
    16081 cctgattaac gtttttgtac accatataac agacgctggg cagcgtcagg gagatggcga
    16141 taaccatcac cgttaaaaac gtggcgaacg gtttgctttt cagatcctgc aatgcgccgt
    16201 ggaaggcata gcgcacctgt tcgttgaaaa cgttggtttt gcgatttacc ggttttggcg
    16261 aggatttcgc gcgtttcggt gcgttacgac cgccgtcgcc tgagccgccg accgatttac
    16321 ggaagcgatc cagacgccca ccaaactgcc gaatatgatt gattgcatcg cgcttattca
    16381 tggcccacgc ctccatgcaa gtgaccatcg ctcagggtga gcatgcgatg ggaacgccgt
    16441 gaaatcaggt tgatgtcgtg cgttgccatc aatacggtta ccccaacgcg gttaaactct
    16501 tcaaacagac gtagaatccc ttccgacaac gcgtcgtcca ggttaccggt cggttcgtcc
    16561 gccagcagta ccgcgggctt attcaccacc gcgcgggcaa tgccaacacg ctgttgttca
    16621 ccgccggaaa gctgaatagg gaagttcttc gctttgtcca gtagcccgac tttatccagt
    16681 gccgccgaca cccggcgacg aatatcgtca ccgctggcac cagcgataat cagcgggatc
    16741 gccacgttat cgtagacagt acggtccatc agtagatggt gatcctggaa aatcatgcca
    16801 atctggcggc gcagaaacgg aacttcacgg tttttcagac gcgtgatgtc atggccgcta
    16861 aaccagattt tcccggcgct gggccgctca atcccacaga tcagcttcag gagggtactt
    16921 ttccctgcgc cggaatgacc ggtcagaaac gccatctcac ccggctgcat atggaacgta
    16981 acgccctgca gcgcctgtct cccaccgaga taagccttgc tgacatgttc aaagcgaatc
    17041 attgttaatc ctctcgggca aaaagtgcct ctataaagtc gtccgcctta aacggacgca
    17101 aatcctcaat acgttcgccg acaccaatgt agcggatagg gataccaaac tggtcagcca
    17161 ccgagaaaat taccccgcct ttcgccgtgc cgtccagttt cgttagcgtg atgccggtta
    17221 agccaacagc ttcgtggaac agtttggcct ggcttaccgc gttctgcccg gtgctggcat
    17281 caatagtcag cataacttca tgcggcgctt caacgtcgag tttcttcatc acgcggacga
    17341 ttttcttcaa ctcttccatc aggtgcgatt tgttctgcag gcgtccggcg gtatcggcaa
    17401 tcaggacgtc gatattacgc gctttagctg cctgaatggc gtcgaagata acagaggcgg
    17461 aatccgcccc ggtatgctgg gcaatcaccg gaatattgtt gcgctgaccc cagacctgaa
    17521 gctgttcaac cgctgctgca cggaaagtat cgcccgccgc cagcatcacc gatttaccct
    17581 gctgctcaaa ctgacgtgcc agcttaccaa tcgtcgtggt tttacccaca ccgttgacgc
    17641 ccaccatcag gatcacgaac ggcgttttac cttcaacatt cagcggctca tcgactttcg
    17701 ccagaatctc acccatctct tctttcagca ggccatagag cgcctcggca tcacgaagct
    17761 gcttgcggga tgcgccttcc gtcagattgg tgataatttt acgtgtggtt tccacgccca
    17821 catcggcgat caaaagctgc tcttccagct cctcaaacag atcatcgtcg atttttttac
    17881 cgcggaacag gctgataaat ccggaaccga gattttcttt ggtttttaac aggctgcgtt
    17941 tcaggcgcgc gaagaaacct tctttggttg gtttttcctg ctcctgagcg atttcttcca
    18001 ccggctgctc ttcttctgcc ggaggaacca ccatcaccgc ctcttctgct gcttcggctg
    18061 ccagcgcctg tgcttccagc tcttcgtcgg tgatttcttc tttagccgct tcttcttccg
    18121 ccgctttgac aatctctacg gtttccgctt cagcctgcca ctcttctggc gaaacctctt
    18181 cggcgttgac gtcttccggc aacggcagct cttcacgttc gatagccact ggctccggcg
    18241 tttcttctac gaccggttcc ggctgtgcaa caacttccgc ttcaggctgc gctttttcac
    18301 tttcaacaac ctgttcagtg acttccacaa cgtcggcagc aaaagtttcc gcctcggctt
    18361 cagtatgcgc ctgcggctgc tcttcaacgg cttgttccga agccttcaca ggctcttgcg
    18421 cctgaacgat ttcttctaca accggttgtt cattctgaac ttctgtctct ttttccgggg
    18481 tctgctcttt ttgaccaaag cccagccagg aaaaaaagcc acgttttttt tctttcgcca
    18541 tttgcgacta cactcctcgc tgttgcttca tggcacagcg ttaacgctat gtacatagca
    18601 gctaaaaaaa tgatgaaata gtctatcact taacttaatt cacatcaccg cctgcaagta
    18661 tgtgttatct ggcggattga gcaatttatc atgaaaaaac cgaatcattc cggcagcggc
    18721 caaatccgca ttattggcgg gcagtggcga ggccgtaaac tcccggttcc tgatagccca
    18781 ggtctgcgcc ccaccaccga ccgcgtacgc gaaacgttgt ttaactggct ggctccggtc
    18841 attgttgacg cccaatgtct ggattgcttc gccgggagcg gcgcgctggg gctggaagcg
    18901 ttatcgcgct acgctgcggg ggccacgtta attgagatgg atcgtgcagt ttctcagcag
    18961 ttaattaaga atctggcgac actaaaagca ggcaatgcac gcgtggtgaa cagcaacgcg
    19021 atgtcattcc tggcgcaaaa aggcacaccg cataatatcg tgtttgtcga tccaccgttc
    19081 cgccgtggct tgttagaaga gacgataaat ttactggaag ataacggctg gctggctgac
    19141 gaagccctga tttatgtcga aagcgaagtc gaaaacggtc tgcccaccgt tccggcaaac
    19201 tggtcgttac atcgggaaaa agtggcgggt caggtggctt atcggctgta tcaacgcgaa
    19261 gcacaaggag aaagtgatgc tgattaatat tggtcgtttg ttaatgctct gcgtttgggg
    19321 atttttaatc ctcaatctgg tgcatccctt cccgcgcccg ctaaatattt tcgttaacgt
    19381 ggcgctgatt tttaccgtgc tgatgcatgg tatgcagctg gcgctattga aatccacttt
    19441 accgaaagat ggcccgcaga tgaccaccgc cgaaaaggta cggattttcc ttttcggcgt
    19501 gtttgaactg ctggtctggc agaagaaatt taaagttaaa aaataacctt attgttcgcc
    19561 aacaaagcta acaaagcgcg ttcctttata gctcagcgta cctttatcgc ccactgtcag
    19621 ggcgtggtac tgctgggcgt cgaggcgaaa cgtctgctcc attcctccgc tttgcggttt
    19681 gaagcttgcc tcgtagcgca tactggtgcc tgccggagtc acttcctgct ggcgcgaacg
    19741 gcgatcgtta atcggttttt cccgcttgtt gctcaccacc accagctttt gctggagcgg
    19801 agccatatca ttatcagctt tttcccgtcg ctgttgcata aaacgaaacg atgcggcgac
    19861 gacaattaag ccaatgataa caataaagaa aagaggtggt ttgctcatct ttatccctca
    19921 tcggaaaatg cggaaataag cataccctgc cagttatggt gttgtcatcc gtccaccctc
    19981 gccactaaac tggaagcaag accgtaggca ttccgcttac gaaaaaataa cgaattcaag
    20041 gaactaagat gctttggtcg tttatcgctg tctgtctttc cgcatggcta tctgtggatg
    20101 catcgtatcg tgggccaacc tggcaacgct gggtgtttaa accgttaacc cttcttctcc
    20161 tgctgttact ggcctggcaa gcgccgatgt tcgacgccat tagctatctg gtgctggcag
    20221 ggctgtgcgc ctcactgctg ggcgatgcgc taaccctgtt gccacgtcaa cgtctgatgt
    20281 acgccatcgg cgcgtttttc ctctcgcacc tgctgtacac catctatttc gccagtcaga
    20341 tgacgctctc tttcttctgg cctctgccac tggtgttgct ggttctgggt gcgctgttac
    20401 tggcgattat ctggacgcgg ctggaagagt atcgttggcc tatctgcacg tttatcggca
    20461 tgacgctggt gatggtgtgg ctggcaggtg aactgtggtt cttccgtccg accgctccgg
    20521 cgctctctgc gtttgttggc gcttcgttgc tgtttatcag taactttgtc tggctgggga
    20581 gccactatcg ccgacgcttc cgtgcggata acgcgattgc tgcggcctgc tactttgccg
    20641 gtcacttcct catcatccgc tcactgtatc tctgataaaa cttgactctg gagtcgactc
    20701 cagagtgtat ccttcggtta atgagaaaaa acttaaccgg agtatgccat gtcgacacct
    20761 gacaatcacg gcaagaaagc ccctcaattt gctgcgttca aaccgctaac cacggtacag
    20821 aacgccaacg actgttgctg cgacggcgca tgctccagct cgccaacgct ctctgaaaac
    20881 gtctccggca cccgctatag ctggaaagtc agtggcatgg actgcgccgc ctgtgcgcgc
    20941 aaggtagaaa atgccgtgcg ccagcttgca ggtgtgaatc aggtgcaggt gttgttcgcc
    21001 accgaaaaac tggtggttga tgccgacaat gacattcgtg cacaagttga atctgcggtg
    21061 caaaaagcgg gctattccct gcgcgatgag caggccgccg acgagccaca agcatcgcgc
    21121 ctgaaagaga atctgccgct gattacacta atcgtgatga tggcaatcag ctggggtctg
    21181 gagcagttca atcatccgtt cgggcaactg gcgtttatcg cgaccacgct ggttgggctg
    21241 tacccgattg ctcgtcaggc attacggctg atcaaatccg gcagctactt cgccattgaa
    21301 accttaatga gcgtagccgc tattggtgca ctgtttattg gcgcaacggc tgaagctgcg
    21361 atggtgttgc tgctgttttt gattggtgaa cgactggaag gctgggccgc cagccgcgcg
    21421 cgtcagggcg ttagcgcgtt aatggcgctg aaaccagaaa ccgccacgcg cctgcgtaac
    21481 ggtgagcggg aagaggtggc gattaacagc ctgcggcctg gcgatgtgat tgaagtcgcc
    21541 gcaggtgggc gtttgcctgc cgacggtaaa ctgctctcac cgtttgccag ttttgatgaa
    21601 agcgccctga ccggagaatc cattccggtg gagcgcgcga cgggcgataa agttcctgca
    21661 ggagccacca gcgtagaccg tctggtaacg ctggaagtgc tgtcagaacc gggtgccagc
    21721 gccattgacc ggattctgaa actgatcgaa gaagccgaag agcgtcgcgc ccccattgag
    21781 cggtttatcg accgtttcag ccgtatctat acgcccgcga ttatggccgt cgctctgctg
    21841 gtgacgctgg tgccaccgct gctgtttgcc gccagctggc aggagtggat ttataaaggg
    21901 ctgacgctgc tgctgattgg ctgcccgtgt gcgttagtta tctcaacgcc tgcggcgatt
    21961 acctccgggc tggcggcggc agcgcgtcgt ggggcgttga ttaaaggcgg agcggcgctg
    22021 gaacaactag gtcgtgttac tcaggtggcg tttgataaaa ccggtacgct gaccgtcggt
    22081 aaaccgcgcg ttaccgcgat tcatccggca acgggtatta gtgaatctga actgctgaca
    22141 ctggcggcgg cggtcgagca aggcgcgacg catccactgg cgcaagccat cgtacgcgaa
    22201 gcacaggttg ctgaactcgc cattcccacc gccgaatcac agcgggcgct ggtcgggtct
    22261 ggcattgaag cgcaggttaa cggtgagcgc gtattgattt gcgctgccgg gaaacatccc
    22321 gctgatgcat ttgctggttt gattaatgaa ctggaaagcg ccgggcaaac ggttgtgctg
    22381 gtagtacgta atgatgacgt gctgggtatc attgcattgc aggataccct gcgcgccgat
    22441 gctgcaactg ccatcagtga actgaacgcg ctgggggtca aaggagtgat cctcaccggc
    22501 gataatccac gtgcagcggc ggcaatcgcc ggggagctgg ggctggagtt taaagcgggc
    22561 ctattgccgg aagataaagt caaagcggtg accaagctga atcaacatgc gcccctggcg
    22621 atggtcggtg atggtattaa cgacgcgcca gcgatgaaag ctgccgccat tgggattgca
    22681 atgggcagtg gcacagacgt ggcgctggaa accgctgatg cagcattaac ccataaccac
    22741 ctgcgcggcc tggtgcaaat gattgaactg gcacgcgcca ctcacgccaa tatccgccag
    22801 aacatcacca ttgcgctggg gctgaaaggg atcttcctcg tcaccacgct gttagggatg
    22861 accggcctgt ggctggcggt gctggcagat acgggtgcga cggtgctggt gacagcgaat
    22921 gcgttaagat tgttgcgcag gagataaggc aaaccgatcg caacattgag cgcgatcggt
    22981 cccctcgccc ctctggggag agggttaggg tgaggggaaa aggcggcatc gaagccaatc
    23041 tttccccatc aaccgccttt acgaatcaaa taacgataag gcagtccatc catctctttc
    23101 gcaaccagtt cgtgttccat aaaggtacaa aacccaggaa tatcgcgggt agtggccgga
    23161 tcgtcggcga taatcagcaa cgtttcgcca ggctgcatat tgcgcacagt tttgcgcacc
    23221 atcattacgg gttccgggca gcgcaggcca agtgcgtcga gtgtgtggtc agggctggaa
    23281 aagagatcgg tcattttctt ctcatcacat aaaaaaacgg cgctagttta cgccctgtga
    23341 gtccgtaagc aaaccaggtt aacgattgcg tgaaaattaa ccattgcatt gtcaacgtaa
    23401 agcagtatca tgcggcggct cgaaaaaagg gtaagcacgt tattatgtta aggtaacaga
    23461 cgtgtcgtac gtattgggtt ccctcacccc aatggttaat caaaaaggta caatatgaac
    23521 gttttctcgc aaactcaacg ctataaggcg ttgttctggt tatcgttatt tcatctgctg
    23581 gtgatcacct ccagtaacta tctggttcag cttcccgtct ccattttggg tttccatacc
    23641 acctggggcg cgtttagctt cccgtttatt ttccttgcta ccgacctgac cgtgcgtatt
    23701 tttggcgcac cgctggcccg tcgtatcatc tttgcggtga tgatcccggc gttgctgatc
    23761 tcctacgtca tctcgtcgct attctatatg ggttcctggc agggattcgg cgcactcgcc
    23821 cacttcaacc tgtttgtcgc ccgtatcgcc accgccagtt tcatggccta cgcgctggga
    23881 cagatcctcg acgtgcacgt ttttaaccgc ctgcgtcaga gtcgccgctg gtggctggca
    23941 ccgacagcgt ccacactgtt cggtaacgtc agcgacacgc tggccttttt cttcattgcc
    24001 ttctggcgta gcccggatgc ctttatggct gaacactgga tggaaatcgc gctggtcgat
    24061 tactgtttca aagtgttaat cagtatcgtt ttcttcctgc caatgtatgg cgtattactc
    24121 aatattctgt tgaaaagact ggcagataaa tccgaaatca acgctttgca ggcgagttaa
    24181 aggttcgtta tcagagttgt gataagatgg atgaatgagc cgttatggcc gtttatcgaa
    24241 aggaagaagt caatgcgcaa tctggttaaa tatgtcggaa ttggcctgct ggttatgggg
    24301 cttgcggcct gtgatgataa agacactaac gctacggcgc agggttcggt cgcggaaagt
    24361 aacgctaccg ggaatcccgt caacctgctt gatggcaagt taagtttctc gctgccagcg
    24421 gatatgaccg accagagcgg taagctggga acgcaggcca ataacatgca tgtctggtcc
    24481 gacgccaccg ggcagaaagc agtcatcgtc atcatgggcg atgatccgaa agaagatctg
    24541 gcggtgttgg cgaagcgtct ggaagatcag caacgtagcc gcgatccgca gctgcaagtg
    24601 gtaaccaaca aagccattga gctgaaaggt cacaaaatgc agcagttaga cagtattatc
    24661 tccgcgaaag gccagacggc gtactcttcc gttattctgg gtaacgtggg taatcaactg
    24721 ctgaccatgc aaattactct gccagctgac gatcagcaaa aagcgcagac caccgcagaa
    24781 aacatcatta atacgctggt tattcagtaa gttttaagat gatgaggcgg cctcagggac
    24841 gtgttccgga ggccgttttt ttaatcgcca cgtcagtaat aacgcgattg cgaccagtcc
    24901 cgccgccgcc agataaatca ccggtacgcc cgcccagctc atcaccagcc cagccagtgg
    24961 tccggtcacg ccaagcgata aatccataaa tacggtgtaa gttgccagcg ccgccccctg
    25021 attttgctgc ggaaccgctt ttaccgccac tacgcccaat gccgggaaca ccagcgaaaa
    25081 ccctgccccc gccagtaaga cgccgacttt cgccatccac ggcatagtcg ccacgccaac
    25141 cagtagcagg ccgattatct caacgctaaa gcaaatcatc gccacgttta agccgccgat
    25201 acggttaatg ccgttaggga ataacaaacg cgtaccgaca aacgcacagc taaacagcgt
    25261 cagcgcgaaa gccgcaccgt cccaaccttt agcgtcataa aacagcgtga taaaggtggc
    25321 gatgacgcca aatccggcag aagccagtgc cagtgccata ccgtacagcc agacgcgccc
    25381 aagcaccgcg cgaaacggca gcggtttgcc tttactggct tttaccgtcg gacgcgggat
    25441 cgccaacaaa atggccacca gcgccacgcc cataatgatt aacgctaacg cctgcaagcc
    25501 gccccagtga taaaacacga cgcctaacgg cgcacccatc gccatcgccc cgtaattgac
    25561 aatgccgttc cacgaaatca ctcgcccgat atgcagcgag ccaaccacgc caacgcccca
    25621 cagggtcgat cccgttccgg caaaactttg cccaatccca aggatcacgc gtcccaggca
    25681 aagtaataac aggctgatga cgggcagact ggcggttaat cctgccgtca gatatcccag
    25741 accgctcaaa aagcagccgc ataaaccgaa gacgacaatc tttttggggc ccagcaaatc
    25801 ggcgtaacgt ccggcatgag gacggctcag caaggtggcg aaatattgca ggctgataac
    25861 caatcctgcc cagaaagcgc taaagcccat cacatcatgg acatagcccg gtaatacagc
    25921 gagcggcaac ccgatggtga ggtagctggc gaagttaaac atgacaatgg agacaatgcg
    25981 caaattcagg cgcaatccgt ttagcgcggg ttcggctacg ggttcgggca tgaggatcac
    26041 cacattttta caacagtgtt tcatttttac cacgtgctga cgtgaaaatc agcagtaaga
    26101 atcagaatat tgctggcgtg actcccgcta cacttaatac aaaaagtcac aaggaagccc
    26161 caatggaaat ccctcaaccc gataaaacgg gcatgcacat tctgctcaag ctggcctcgc
    26221 tggtagtgat cctcgcgggc attcacgcag cggcagatat cattgtgcag ctgttactgg
    26281 cgctgttttt tgccatcgtc ctcaacccgc tcgttacctg gtttattcgt cggggagtac
    26341 aacgccccgt tgccattacg attgtagtgg tggtgatgct gatcgcacta accgcgctgg
    26401 tcggcgtact ggcggcatcg tttaacgaat ttatctctat gctgccgaag tttaataagg
    26461 agctgacgcg caaacttttt aaattgcagg agatgttgcc ttttcttaat ttgcatatgt
    26521 cgccggagcg aatgctgcag cggatggact cggaaaaagt ggttaccttc accacagcgc
    26581 taatgaccgg gctttccggg gcaatggcga gcgtgctttt gctggtgatg accgtagttt
    26641 ttatgctgtt tgaagtgcgc cacgtccctt acaaaatgcg ttttgcgctg aataacccac
    26701 agattcacat cgcaggatta caccgcgcac ttaaaggcgt ttcgcactat cttgcattga
    26761 agacgctact cagtttatgg acaggtgtca tcgtctggct ggggctggag ctgatggggg
    26821 tgcagtttgc gctgatgtgg gcagtactgg cgtttttgct caactacgtg cccaatatcg
    26881 gcgcggtaat ttccgccgta ccgccaatga ttcaggtgct gctgtttaat ggtgtttacg
    26941 aatgtattct ggtcggcgca ttgtttttag tggtccatat ggtcatcggc aatattttag
    27001 aaccacggat gatgggccat cgcctgggga tgtccaccat ggtggtattt ctttcattgt
    27061 taatttgggg atggctgctc ggcccggtag ggatgctact ttcggtgcca ttaaccagtg
    27121 tgtgtaaaat ctggatggaa accaccaaag gcggtagcaa actggcgatt ttactggggc
    27181 caggcagacc gaaaagtcgg ttaccgggat gaggcgacaa gtgatacgat acgcactttc
    27241 attttccatt aaacgttggc cctgatatgt atcggatagt tctggggaaa gtttcgacct
    27301 taagcgcagc tccactgcca ccgggtttac gcgagcaagc accgcaaggt ccacgacgcg
    27361 aacgctggct ggcggggcgt gcattgcttt cgcacacgct ttccccgcta ccggagatca
    27421 tctatggcga acaaggcaaa cctgcattcg cgccggaaac gccgctatgg ttcaacttaa
    27481 gccatagcgg tgacgatatc gctctgctgt tgagcgatga aggcgaagtc ggctgcgata
    27541 tcgaagtgat tcgcccgcgc gccaactggc gctggctggc gaacgccgtg ttcagcctcg
    27601 gggaacacgc tgaaatggac gccgtgcatc ctgatcagca actggaaatg ttctggcgca
    27661 tctggacgcg caaagaagcc atcgttaaac agcgtggcgg cagcgcctgg caaatcgtca
    27721 gcgtagatag cacctatcac tcctcgctgt cagtcagcca ttgtcagcta gaaaatttaa
    27781 gccttgcgat ctgcaccccc acccccttta cgctcaccgc cgacagtgtg caatggatcg
    27841 attcagttaa ctgatccgcc cacccgactg cccatctatt gatccagaac aggtaatcag
    27901 tatgacgaat acttaaaatc gtcatactta tttccgccat ctattttaat ccattggggt
    27961 taccatgctc tccacactcc gccgcactct atttgcgctg ctggcttgtg cgtcttttat
    28021 cgtccatgcc gctgcaccag atgaaatcac taccgcctgg ccggtgaatg tcgggccact
    28081 aaacccgcac ctttacacgc ctaaccagat gttcgcccag agcatggttt atgaaccatt
    28141 ggtgaaatat caggcagacg gttcggtgat cccgtggctg gcaaaaagct ggactcattc
    28201 agaagatggt aaaacctgga ccttcaccct gcgtgatgac gtgaaattct ccaacggtga
    28261 accgttcgat gccgaggcgg cggcagaaaa cttccgcgca gtgctcgata accgtcaacg
    28321 tcacgcctgg ctggagctgg caaaccagat tgttgatgtt aaagcactca gtaaaacaga
    28381 gctgcaaatt accctgaaaa gcgcctacta tcctttcctg caagaactgg ccctgccccg
    28441 tcctttccgt tttatcgctc cctcgcagtt taaaaaccat gaaaccatga acggaattaa
    28501 agcgccgatt ggcaccggac cgtggatttt gcaggaatcg aaactgaatc agtacgatgt
    28561 cttcgtccgt aacgaaaact actggggcga aaagccagcg attaaaaaga tcacctttaa
    28621 cgtcatcccg gacccgacta cccgcgcggt ggcgtttgaa actggcgata tcgacctgct
    28681 gtacggaaac gaagggttat taccgctcga taccttcgcc cgctttagcc agaatccggc
    28741 ttaccacacc caactgtcac agccgatcga aaccgtgatg ctggcgctca ataccgccaa
    28801 agcccccacc aacgagctgg cagtacgtga agctcttaat tacgcggtaa acaaaaaatc
    28861 gctgattgat aacgcgttat atggcaccca gcaggtcgcc gacaccctgt ttgccccttc
    28921 tgtgccttac gctaatctcg gcctgaaacc gcgccagtac gatccgcaga aagcgaaaga
    28981 gttgctggaa aaagccggtt ggacgctgcc tgcgggcaaa gacatccgcg agaaaaatgg
    29041 tcagccgctg cgcattgaac tgtcgttcat cggcaccgat gcgttaagca aatcgatggc
    29101 ggaaatcatt caggctgata tgcgccagat tggcgcagat gtctcgctga ttggcgaaga
    29161 agagagcagt atctatgctc gtcagcgcga cggtcgtttt ggcatgattt tccaccgcac
    29221 ctggggcgcg ccatacgatc cacacgcctt cctgagttca atgcgcgtac cgtcgcacgc
    29281 tgacttccag gcacagcaag gattagccga caagccgctg attgataaag agatcggcga
    29341 agtgctggcg acccatgacg aaacgcaacg tcaggcgctg tatcgcgaca ttttgacccg
    29401 tctgcatgac gaggcggttt atctgcctat cagctacatc tcgatgatgg tggtatcaaa
    29461 accggagctg ggtaatatcc cctacgcgcc gatcgccacc gaaattccgt tcgaacagat
    29521 taaaccggtg aaaccttaat gttgcgttac gtattacgcc gctttctgct gctgatcccg
    29581 atggtgctcg ccgcctcggt gatcattttt ctgatgctgc gcctcggtac cggcgacccg
    29641 gcgctcgatt atctgcgttt atctaacctg ccgccgacgc cggagatgct ggcctctacc
    29701 cgcaccatgc tcgggctgga tcagccgctg tacgtccagt acggtacctg gttgtggaag
    29761 gcgctgcatc ttgactttgg tatctcattc gccagccaac gcccggtgct ggacgatatg
    29821 ctgaacttcc tgcccgccac gctggaactt gcaggtgcgg cgctggtatt aattctgctc
    29881 acttccgtac cgctcggtat ctgggcggcg cgccatcgcg accgtctgcc ggatttcgcc
    29941 gtacgtttca tcgcgtttct tggcgtgtcg atgcctaact tctggctggc gtttttactg
    30001 gtgatggcgt tttcggtgta tctgcaatgg ctacccgcga tgggttacgg cggctggcag
    30061 cacatcattt tgcctgcggt ttccattgcc tttatgtcgc tggcgattaa cgcgcgactg
    30121 ctgcgcgcca gtatgctgga cgtcgccggt cagcgtcacg tcacttgggc gcgtctgcgc
    30181 ggcctgaacg acaaacagac cgaacgccgc catattctgc gcaatgcctc gctgccgatg
    30241 atcaccgccg tggggatgca catcggcgaa ctgattggcg ggacgatgat tatcgaaaac
    30301 atctttgcct ggccgggcgt cgggcgctat gcggtgtcgg cgatttttaa ccgtgactat
    30361 ccggtgattc agtgctttac gctgatgatg gtggtggttt ttgtggtctg taatttgatt
    30421 gtcgatttgc tcaacgccgc gctggacccg cgcattcgtc gtcatgaagg agcgcacgcg
    30481 tgaacttttt cctctcttcc cgctggtcgg tacgcctggc gctgatcatt atcgccctgc
    30541 tggcgctgat tgcgctcacc agccagtggt ggctgccgta tgacccccag gcgattgatt
    30601 tgccgtcgcg cctgctttcg ccggatgcgc agcactggct gggcaccgat cacttaggtc
    30661 gcgatatttt ctcgcggctg atggcagcga cccgcgtgtc gctcggttcg gtaatggcct
    30721 gcctgctgct ggtgctgaca ttagggctgg ttattggcgg cagcgccggg ttgattggcg
    30781 ggcgcgttga tcaggccacc atgcgcgtcg ccgatatgtt tatgaccttc ccgacctcga
    30841 ttctgtcgtt ctttatggtt ggcgtgctcg gcaccgggct gaccaacgta attatcgcca
    30901 tcgccctgtc gcactgggcg tggtatgcac gcatggtgcg cagcttggtg atttcactac
    30961 gccaacgcga gtttgtgctg gcgtcacggc tttccggtgc gggccatgtg cgggtgtttg
    31021 tcgatcatct ggcaggcgcg gtgatccctt cgctgctggt gctggcaacg ctggatatcg
    31081 gccatatgat gctgcacgtc gcggggatgt ctttccttgg cctcggtgtg accgcgccga
    31141 ccgccgaatg gggcgtgatg attaacgacg cgcgccagta tatctggacc cagccgctgc
    31201 aaatgttctg gccggggctg gcgctgttta tcagcgtgat ggcctttaac ctggtgggtg
    31261 acgcactgcg cgatcatctg gatcctcatc tggtgacgga gcacgcacac taatgccgca
    31321 acagattgaa ctacgtaata tcgcgctaca ggccgcgcag ccgctggtac acggtgtatc
    31381 gttaaccctg caacgcgggc gcgtgctggc gttagtcggc ggtagcggca gcgggaaatc
    31441 attaacctgc gccgcgacgc tgggcatttt gcccgctggc gttcgccaga cggcggggga
    31501 aattttagcc gatggcaaag cggtttatcc ctgcgccctg cgcggcatca aaattgccac
    31561 catcatgcag aacccgcgca gcgcctttaa tccgctacac accatgcaca cccacgcgcg
    31621 ggaaacctgc ctggcgttag ggaaacccgc cgatgacgct acgcttaccg ctgccataga
    31681 agcggtgggg ctggaaaacg ccgcgcgcgt gctgaagctg tacccgttcg agatgagcgg
    31741 cggcatgttg cagcgcatga tgattgcgat ggcggtgctg tgtgaatcac cgtttatcat
    31801 cgccgatgaa ccgaccaccg acctcgacgt ggtagcacag gcgcgcatcc tcgatctgct
    31861 ggaaagcatt atgcaaaaac aagcgccggg aatgctgctg gtcacccatg atatgggcgt
    31921 tgtggcgcgt ctggcggatg acgtggcggt gatgtcacaa ggtaaaattg tcgaacaggg
    31981 cgatgtagaa acgctgttta acgcccccaa acatgcggta acgcgcagcc tggtttccgc
    32041 tcatctcgcc ctctacggta tggagctggc atcatgactt tacttaacgt ctccggcctt
    32101 tcccatcact atgcccacgg tggatttaac ggaaaacatc aacatcaggc ggtgctgaat
    32161 aacgtttccc tgaccctgaa aagcggcgaa accgtcgccc tgctggggcg cagcggctgc
    32221 gggaaaagta ccctcgcgcg gttgctggtg ggtttagaat cgcccgcgca ggggaatatt
    32281 agctggcgtg gcgaaccgct ggcgaaactc aatcgcgccc agcgtaaagc gttccgccgc
    32341 gatattcaga tggtatttca ggactccatc agcgccgtga atccgcgcaa aaccgtgcgc
    32401 gagatcctgc gtgaaccgat gcgccacctg ctatcactga aaaaatccga acaactggcg
    32461 cgcgccagcg aaatgctgaa ggcggtcgat ctcgatgaca gcgttctcga caaacgccca
    32521 ccgcagttaa gcggcggcca gctccagcgc gtctgcctgg ctcgcgcgct ggcggtcgaa
    32581 ccgaaactac tgattctgga tgaagccgtt tctaaccttg atctcgtgtt acaggcgggc
    32641 gtcattcgcc tgctgaaaaa gctacaacaa cagtttggca ccgcctgcct gttcatcacc
    32701 cacgacttac gcctggtgga acgcttctgt cagcgggtga tggtgatgga caacggacaa
    32761 atcgtcgaaa cccaggtggt gggagataaa ttaacctttt cctctgacgc cggacgtgtg
    32821 ctacaaaacg cggtattacc cgcattcccc gtgcgccgtc gcaccacaga aaaggtttaa
    32881 cgcaaatgca acgagtcacc atcacgcttg atgacgattt actggagacg ctggacagcc
    32941 tgagccagcg tcgtggttat aacaaccgtt ccgaagctat ccgcgatatt ctgcgtagcg
    33001 ccctggccca ggaggccact cagcagcacg gcacgcaagg tttcgcggtg ctctcctatg
    33061 tgtatgaaca cgaaaaacgc gacttagcca gccgcattgt ctccacccag caccatcacc
    33121 acgatctctc cgtcgccacg ctgcatgtgc atatcaatca cgacgactgt ctggaaatcg
    33181 ccgtgctgaa aggtgatatg ggcgacgtgc agcattttgc cgatgacgtt atcgcccagc
    33241 gcggcgtgcg gcatgggcat ttgcagtgct tgccgaagga agattgagtt atttctgaaa
    33301 cgaggaaaga aaagatatta actgaactta atttataaaa ttaagttcag ttgcatagat
    33361 agatcctgta aaggcagata ttctgcgtaa tgtacgccgc taaaatttat aaatgattca
    33421 aacgctcatt cagtgtttca ataatatagg tattaacaga gacttgttgc tgcgcagccg
    33481 cattattaag gcgttcactc agcgattctg gatagcgtaa cgtaaaggtc ttaatctttt
    33541 ctgtacgcgc atacggctca atacccgcag ctttacaatc ctcaagatac tctcgcaggg
    33601 aaagttcccc ttctttttgc agcccctgaa tgctgtcaga gacaaaatca caataacctg
    33661 acaagcccag gaatttgcca cgaaatgcat tcagttctgg cacgtaggta ataacagctg
    33721 gttgcccggc aatctccata gagtttggcg tcttcagttt gatcatggtt ttacccctat
    33781 acttataacc agtcacgcac gctttctaca gcgcctttat cggtatctgg cgacggatga
    33841 ggccgatgga aacacgcaac gctcatattt agtatgaact tacaacgcga acctcttcct
    33901 tccttaattt ctccgcctaa tgctttaacc agtgactcta tatcagacca cttgatccct
    33961 tgaggaacag gagttttaaa tatttgatcc aatgtatttt ttttcttccg taaagcctgt
    34021 accttggttc tcatggcatg tccttatgac ttcactttat gacatcaata gtatgacatc
    34081 acagtatgaa gtcaagtttc ccttccttac gccattgtcc caatcgtctt cctgaatcgc
    34141 agcagcgcaa tggtgaaaaa cgcgccgcca attgccatca gcgtcaggaa ctgcggccag
    34201 acgatttcga atccggcacc ccggtagaga atggcctgcg cgaggctaac aaagtgtgtc
    34261 gtcggcatgg tcagcataat gtcctgcacc atctgcggca tactttcgcg cggcgtggat
    34321 ccaccggaaa gcatttgcag cggcagcagc accagaatca ccagcagccc cagttgcggc
    34381 attgaacgcg ctatcgtccc cataaaaatg ccgattgacg tggtggcaaa cagactgagc
    34441 gccacgccca gcataaacag cgggatcgag ccttcaatcg gtacgcccag tacacctttc
    34501 gccatcagca ccagcgataa tcccgatacc accagcacca ccagccccat cgaccagatc
    34561 ttcgccatca tgatctcaaa cggcgttatc ggcatcacca gtaagtgttc caccgtgccg
    34621 tgttcacgct cgcggatcag cgccgatccg gtcaatacaa tcgccagcat ggtaatgttg
    34681 ttgatgatcg ccatcacccc gccaaaccac gcgggatcga ggttcgggtt aaagcgcatc
    34741 cgggtttcca gcgataccaa cggttcgctg ttatcacggt agcgcgcgac aaagctgttc
    34801 acttcaccgt tgataatatt ctggatatac ccattgccgg taaatgcctg gctcatgcgc
    34861 gtggcatcga cgttcacctg aatatccggc tggcgtccgg cgaggacatc acgctgaaaa
    34921 ttaggcggaa tatttatcgc gaaggtatag cgtccggcgt ccagtccggc atccatctca
    34981 tcggcggtga tcatctccgg tggcaaaaac cacggacgat agaagctgtt aacgatccgg
    35041 ttcgataact gcgattgatc catatcggca atggcgatcg gcgcgaggtt caacgatcct
    35101 ggcgtaacgg tcgctgacga atacaccgac accgtaaacg agaagacaat cagcgtcagc
    35161 atcgctttat caccgagcag actgcgcaac tctttgatac ccagattaaa aatattgcgt
    35221 aaatggcgca tcatccctcc tgttttttca gcagcaggat gcttaagccc atcaccagcg
    35281 ggatggctat cagcaacggg ataaaaagtt gccacaaatc agtcagatcc agcgctttcg
    35341 agaacgttcc gcgggcgata gtcagaaaat gactggtcgg gtaaacctcg ccgatccaac
    35401 gtccaggccc ttccagcgaa gctaccggat cgatcatccc ggaaaactgt gtcgccggga
    35461 tcaacgtgat aatcgccgtt ccgaaaatgg cggcaatctg gcttttcata aaggtggaga
    35521 tcagcagccc cattccggtg gcaatgatga tatacagcag cgccgccagg gtgagcgtca
    35581 ggaaactgcc tttatgcggt acgccaaaca caaacaccga caggccgcag agcaggaaaa
    35641 agttcagcat ccccagcgcg atgtacggca gctgtttgcc aagcaaaaat tcgctacggg
    35701 tggtgggggt cacgtaaagg ttgataatcg accctagctc tttttcccgc accacgctaa
    35761 gggcgcttag cattgacggg atcatcatca gcagaagcgg gatcaccgcc ggaacaatcg
    35821 ctggcaggct ttttacgtcc gggttatagc gatagcgcgt ctcaatgttc atcagcccgc
    35881 tttggctggc gggtgtcgat tgtcggctcg ccacatcctg aagccaactc tggtgcattg
    35941 cctgcacgta accttttacc gtttcggcac gactgggcat cgctccgtcg atccagacgc
    36001 cgagttccac aggcgtacca cgcgcgatat cgcgcccgaa attgggcggg atctcaatcg
    36061 ccaccgtgat atcgcccgca cgcatccgac gatcaagctc gtcataactg gtgagcggcg
    36121 gctgttcgat aaagtaacgg gaaccggaga ggttgagtgt ccacgcctgg ctactgacgg
    36181 tctggtcgcg gtcgagcacc gcaaagcgca ggttttccac atccatactg atgccgtaac
    36241 ccattatcag catcaggatc accgttccca tcagcgccag cgtcgaacgt actggatcgc
    36301 gtcgcagttc cagcgcttcg cggcggctgt agctaaacag acggcgcagg ctaaatccct
    36361 gacgcggcgc gtgggtggtg tcgtgtacca cgggcggcgc ttcggcttcg ttgctctgcc
    36421 ctgccgcttc ctgcaaatag gcgataaatg cctcttccag actggcggct ccgcgtttct
    36481 caaccagttc ctgcggtgta ccgctggcaa gcacttttcc ggcgtgcatc agtgagatgc
    36541 ggtcgcaacg ttccgcttcg ttcataaagt gggtagagat gaagatagtc actttgtcct
    36601 ggcgcgagag atcgaccatc aactgccaga acatatccct cgccaccgga tcgacaccag
    36661 aagtaggctc atcgaggatt aacatctccg ggcgatgaat caccgccacc gccagcgaaa
    36721 gccgctggcg aatgccgagc ggcaatgact ccggcagaac atcttcaacg tcgttgagct
    36781 taaaacgctc gctcatttca gccactcttg cgggaatttc cgcttccggg atgtgaaaca
    36841 aacgggcatg taactcaagg ttttgccgca cggtgagttc gttatagagc gaaaacgcct
    36901 gcgacatata gcccacccga cggcgggtat cgatatcttt tggatcaacc ggttgcccga
    36961 acagccacgc ctcaccttcg ctggcgggca gcagtccggt gagcattttc atggtggtgg
    37021 atttaccgca gccgttcgaa ccaagaaaac caaaaatctc cccgcgtgga atgcggaaat
    37081 taacgtgatc aacggcaacg aaggaaccaa aacgcatggt cagatcgcgc gcttcgatgg
    37141 caatctctgc gttttcaggt tgatacggtg ggatcactac cgcctgatgc gcctggcgtt
    37201 gcgcttgcgg taacagattt ataaatgctt cttccagcgt agcgctttgc gtttgctgcc
    37261 gtagctcttc ggcgctgcca gttgccagca cttctccggc attcatcgct accagccagt
    37321 cgaagcgttc ggcctcttcc atataggcgg tggcgaccag cacgctcata ttgctctgcc
    37381 gctggcgaat actgtcgatc agatcccaga actgggagcg ggagagcggg tcaacccccg
    37441 ttgttggctc atcaaggatc aacagttccg ggtcgtggat taacgcgcag cacagcccaa
    37501 gtttttgctt catcccgccg gagagtttcc ctgccggacg atcgcgaaac ggtgctaacc
    37561 ccgtgctggt cagcagctca ttaattcgca cttcccgctc cgctttgtcg tgaccgaaca
    37621 ggcgagcgaa aaaatcgacg ttttcataca ccgacaaggt gtggtagagg tttttgccca
    37681 gcccctgcgg catccaggcg atgcgcgggc agacgtcgcg gcgatgcctc gggtcgcgca
    37741 tatcgccgcc cagcaccatc acattgccct gctcaatgac gcgggcaccg gaaatcaacg
    37801 acaataagct cgacttcccg acgccatccg gaccaatcag cccgaccata cagcgggccg
    37861 gaatatcgag agtaatattg ttcagcgcaa cgttttttcc ataatgctgg ctcacgcccg
    37921 ccagttgcgc gacaggcggg acgggaacca gttccagatg cgtcattgcg gcaacctcac
    37981 cacgaggtcg tcaggccacg gaagttcttc attcacccgc acccacgcta cgcccggtaa
    38041 accggttttg acatattcca gatgctgctg gagtaattcc ggtgggatac gcgctttgac
    38101 gcggaacatc agtttcagcc gttcatcgct ggtttcgacg gtttttggcg tgaactgggc
    38161 gacactggcg acaaaactga tggtcgccgg aatacgcaga tctggcgcgg catcgaggat
    38221 cagccgggct tcaccgccaa gtttcagcgt gcccgcctgt tcggttggca ggaagaaagt
    38281 catatagacg tcgctgagat cgaccatatt cagcacccga ccgcctgccg ccagcacttc
    38341 ccctggctca gcaacccgat actgcacgcg tccgtcacgc ggggctttca gttcactgtc
    38401 atcgatatct gcggcaatac gccgttcagt ggcttgcgcg gcttcgacgc gagtttgggc
    38461 ctgaatgata ttggtacggg ccgcttctat agccgcttta gaagccgata cctgagcttt
    38521 tgccgattcc agcgcagctc gggcgctctc agcggcggcg cgatcgtcat ccagctgttg
    38581 cgcagaaata gcccctcgtt gggcgagcga gcgggaacgc gtatgacgtt tagccacgga
    38641 gtccagttcg gactggcgtt gattaaccag cgactgcgcg gcacgagttt cgctttgccg
    38701 ttgctccagc aaagcctgcg cggcagcaac ggcactttgt gcttctttga tttgcgcgat
    38761 ggcttccagt cgctgctcct gcaacacgcg agtatccatc ttcgccagca cttcgccttc
    38821 gcgaacaaac tggccttctt tcaccagaat ggtgtcgata cgcccggcaa ttttgctggc
    38881 gatatccact tccgtcgctt caatgcgccc attgctgaca gcaaagcctt ccggcacacc
    38941 cgccgggcgc aacagccacc acgccacgac ggcagccacc accagtaacc cgacaaccca
    39001 ccacgccaga tggcgcttac tcttatccat aatcgacccg ccataatccc tgcaagcaaa
    39061 cgacgcgtgt tacaactgcc gccgaacgac gttcagcgga ttcttccatc cgggataaga
    39121 aggaaacgac aaatacgacc agcgcgcaga gcattgagcg ccaacgggga aggcttagtg
    39181 aaaacgggta gtaatgttgt catcatcagc attcctggcc gtaaatgaaa cacgcatact
    39241 cataagtgtg atctataaaa ctggctgaca aaacggtatc catcctgttg gatagcgttt
    39301 cacgcctgtt cagttgcagg aacagagcat tcctttttac gttttatcaa cagtctcaac
    39361 cagatagtaa cattaatatt catgttgtct atggttcaga gctgtaacat tgtgcgcctt
    39421 ctgatcaaag gcataagtcc atttctgttt ttttcatgct ggtaagactt ccagggagtg
    39481 gttatctcag gccctgcacc agggttaatt tggcaattat gcatgctgaa cattcttcca
    39541 gcatgtttac tgtcacgggc gttaagacct aaaactgtcc ccgtttcacc tatacattct
    39601 cttccaccat taccgttatg tgcaacaact tgatttatac ctaacatttg tgagcatgct
    39661 aatagtgctt caagtttagg tctgtaatct gtcgaaacag aaaaataacg attaaaggat
    39721 cgacaagaga tagcccggct atttttaaac tttttgccat taattacagc atggtgcttt
    39781 ttattgataa ttgccgctaa ttcgaccgga tcgagagcat tggtcatttc atcactagag
    39841 actgttatcg gtcctataat gtaacgtttt cgctgatcat caaaagttat cccatgatga
    39901 tttgccatta ttttgtattt ggggtcatag aaacatacag gataatcttt tatcccagag
    39961 taggtatcct catcaagtgg tttcatgcgc gtgaagttct gcatatagtt accattgcaa
    40021 ttcgtttcat gatttccagc taatacatga acgttttcat tgatactcat tatgcgtttt
    40081 aataaatcga tgataaattt atcgccacag atattactaa atcgatcgcc agtctggtcg
    40141 cccagaaacg tacatggcgt gactgcgtct ttattaataa ccaaataagg ctgaagactt
    40201 tctaaaacaa tgtctcttct gttcaggata ctgattatta tattaacatt agtgtcaagt
    40261 tgctcattaa agttctccct tagcaatacg ttgagaactt catcttctat attaagcaac
    40321 tggctaagga aattttctcc ttcagccatg attcccaaat gtcccgttgc aaaaagcgcg
    40381 tacaaaactg caccgactga gccatcggta tcgccaaaat aggtcggtct ggtacttgtg
    40441 ggatcaacag gtgaacaatt ggagaaatag gttctgtcag cgaactgggg ttttatcatt
    40501 gtttcgaatt taatatcaaa tacattgtat tgcgatattt tccagttcca gatgggtttg
    40561 tacgactcag taactgcaac atcattagca ttatcattta ttgtcatatt attaagtaaa
    40621 taaacaatgc tgcaccaatt aatatcggta ttatcaagag ggcaaccgta atttttacta
    40681 ttaattgtca caataaactg ataacagtta ttcctacgta gaatatgagc gcgcatgccc
    40741 gatagcatac tatatttttc tgaatgattc gtttgtatgg taaatgaaat tttgtcatct
    40801 tgaatagatt gttggaaccg ttctatttgc caatcatcta ttttttccac aacctcaccg
    40861 agcattgtcc tggcaatact tgatatatca tcacctatac catttgcaat atacccaaac
    40921 agttctttag tgaagttggt aacatgctct tgcaccatat catttgttgt gattgtcgtt
    40981 gtactgtcgt tagtgcctgc cacagttcca attttcatct tttacctcat attatttacc
    41041 gcgaggaata catagttaac atttaatctt atgacataca cctcatgttc gatgggaaaa
    41101 taattatatt ttgcacaaat cattcagcct taaagcataa tatgaaattt cttatctcat
    41161 agcagagaaa gtccctaaag attgtagagg cgtcatcaga acatgcctct acaatcgatt
    41221 aagaaaacct atgaaatact ggcaaaatta aaaagagata ccacagcact catgaagttt
    41281 tattgaggtt gaaaacaact aagccctggg acaccttttt tatcaccacg ttcgttaggt
    41341 gctatcttcc aggaatttag ccccatggta taattatcaa ttttttgatg ctgatctacc
    41401 cctaatccat aatggccata tttctgttta gtttttttga gcgtctgtcc acatttgaag
    41461 atatcttcag gttttggtct gaaagcagta gtgttattaa aataattttc atatacaggt
    41521 gtggatgcta cagcattgct tctaattaga tggataagac gatcatccat gtgttgttta
    41581 tgttttttat taaatatatt tgctaattcc tcaggatcaa ttgggtcttt catctgatta
    41641 aatggaacct gcaaagcacc gagacaataa cattcatttt cttcatttct tattattccg
    41701 tgatgactgg ttaaaacttt cgtttctgag tcataatgac aaacatcgag cgttttaatt
    41761 aagttatacg tatcaccggc agagagttta taattttcta gtcgtgcagt atagttacca
    41821 ttaaaattga tttcatgatt tccagccaga acgatgacat ttttattaat acggctatct
    41881 ttattaccat ccatatttcg catagaattt agcaaagtaa ggatatattt atcaccaaaa
    41941 atagtactaa atctatcgcc tgtatgatcc ccaaggaaga tacaaggggt tatcgcatcg
    42001 tcctttaaaa aaacatgttg atgcaactca ttcaatatca tgtctctcgt atttaataaa
    42061 ttgtagagca atttattatt tttatgtatg tatgcagatg aagccatgtt ttcgtgtttt
    42121 aataattcac ataataaact ccaacccttt tccctgatgc ctatatgccc tgaaacgagc
    42181 aatgcataaa gtacagcgcc tactcttcca tctgtatcgc caaaataagc cgtgttggca
    42241 ttttgtgggt taatcacacc acattgttta taaaagtcta ttttagaaaa cacagattta
    42301 acatagctat caaacttatc cttaggaaac gtctctttag agacatccca acgccagtca
    42361 gctttaaaat taccgctaag aagagtaaag aaattttcag gtatgataga aagcccatta
    42421 actttataag ttatgtctga tttataaatt atgtctgatt tatctccgga gaaattaata
    42481 aaataataac catccttaca ctcaatctta actgtaatgg gagacgataa ttttatcaca
    42541 tggttattgt ctggtataat tgtaaagata aacgtgttag tagtattatc ggcgaaacta
    42601 ttatattctt gaatatctat agatggctca ctattgtaaa accatatttt ctccatctga
    42661 agactatgtt catttaccct ccagtgacca tgttgcagga aatagatgat attcaggcct
    42721 aaatcgttat ctatttgcga aaagtcattc agcatattaa cttcaactgt gtttgatcga
    42781 tattttttat caaccttgcc atggtgttga tgactgttaa ctttatctga atgcttatct
    42841 tgatggtttg aagagaagtg cttaaatata ttgtgaaata ctttcataaa taacctgtaa
    42901 tatttttact tgttagtaac aaaataaaca atacatgtct gaaagacata tttacgatga
    42961 tacaaagcaa taatacttat atgttcgatg attttaagca ccgagactat ccggtctttt
    43021 actaaataat ggaaaacaat ccatccctgg aagataattg taaaaaaaaa caacagaata
    43081 agaattaatc ttaggataaa tatttattta tcatggcctt ttaaccggta aaaaatggca
    43141 ctcggcacag taaaccccgc tatagttatg ctatgcattt gtctttaatg atatctcgtc
    43201 gttatattaa caggctgaaa ttatcattaa tgcacatttc attattagca gggatacctg
    43261 aggagtttaa agtgaacata tatatcgggt ggcttttcaa attaatccct ttgattatgg
    43321 gattaatttg catcgcgtta ggtggctttg tgctggaaag ttcagggcaa agcgagtatt
    43381 tcgtcgcggg tcatgtgctg atttctctgg cggccatatg cctggcatta ttcactaccg
    43441 catttattat catttcgcag ctcacgcgcg gcgttaatac gttttacaat acattgttcc
    43501 ccattattgg ctatgcgggg tcaattatca ccatgatatg gggttgggca ctgttagcag
    43561 gcaatgatgt gatggcagac gagtttgtcg ccggccatgt tattttcggc gttggtatga
    43621 ttgccgcctg tgtatcgacg gtggcagcgt catccggtca ctttctgctc attcccaaaa
    43681 atgcagcggg gagcaagagc gatggaacgc cggtacaggc ttattcttca ttaatcggta
    43741 attgcctcat tgccgttccc gttttactca ccctgctcgg tttcatttgg tctattacgc
    43801 tgttacgtag tgctgacata actccgcatt atgtcgcggg tcacgtattg cttgggttaa
    43861 ccgcaatctg tgcctgtcta attggccttg ttgccacaat tgtccatcaa acacgtaata
    43921 cgttttcaac taaagaacac tggctgtggt gttattgggt tatttttctc ggctcaatca
    43981 cggtactgca ggggatatac gtcttagtca gttccgatgc aagcgcccga ctggctcccg
    44041 gcattattct tatttgcctc ggaatgatct gttacagcat attctcaaaa gtctggctac
    44101 tggcactggt ctggagacgt acctgttcgt tagccaacag aataccgatg atacccgtct
    44161 tcacctgcct gttttgcctt ttcctggcat catttcttgc ggaaatggcg cagaccgaca
    44221 tgggatattt tattccttcg cgagttctgg tcggtttggg agcggtatgc tttacgttgt
    44281 tctcaatcgt ttcaatatta gaagcgggtt ctgctaaaaa ataattgcaa cgtaccggat
    44341 aaaaccagcg ttgaccattt gcgtaacgct ggtttttctt aggcatcatg aaataacgca
    44401 gcattaatgc atagtggtta aggataaaaa aacaacaaag ttactgtctt tttcagcctg
    44461 ttcatatttt ataaagatct ggccactggg tgagttttca atcacctttc cttccacctt
    44521 atattaagcc tggagggttt cagttcacgg gctcattaga aaataatcac aagatagcca
    44581 ctatcaaaat tagctcattt ttaatgcggc tctaataatt ttcatcttta ggtaacaggt
    44641 caggacgact tcgctgcaat caaatcctgc gcacaagccc acgcactcga ccacgcccac
    44701 tggaagttat agccccccag ccagccggta acgtccatca cttcgccgat gaagtacagc
    44761 ccaggcactt tgcgcgcttc catcgtccgt gaagagagtt cgttggtgtc cacgccgccg
    44821 agcgtcactt cggcagtgcg atagccttca gtgccgttgg gttgtacgcg ccagtcggtc
    44881 aatgtgctaa tcagtgcctg ttggtcacgc acgttgagct gttttagcga aacattcggg
    44941 atttgcccga gttgctgtaa gcgttcaacc aaccgcttcg gtagatgaac cgccagtgtg
    45001 tttttcaggc tctgattcgg atgtgcgtta cgctgctcat tcaggaaggt ttcgagatcc
    45061 acatccggta gcagattgat actgacaaat tcacccggtt gccagtagct ggaaatctgc
    45121 aacaccgccg gtccagacaa gccgcgatgg gtgaagagta agttctcacg gaaaacgatg
    45181 ccgttttcag cggtaatcac ggaaggcacc gccacgcccg ccagcacctg taactcttcg
    45241 agcaacggtt tatgcagagt gaatggcacc agacccgcac gggtcggcag cacgttgagg
    45301 ccaaattgtt cggcaatctt ataaccaaac ggcgacgcgc ccagccccgg cattgacagc
    45361 ccaccggtcg ccatgaccag cttttcgcaa ccgacagtca tgccgttcag atcaagcgtg
    45421 aagcctgttt catcctccgc cacactcagc acttcgctac gcaatctgaa ggtcacattg
    45481 cccttctcgc actcatccac cagcatgtcg acaatctgct gcgcggagtc atcgcagaag
    45541 agttgcccta acgttttctc gtgccatgcg atgccgtgtt tattgaccag atcaatgaaa
    45601 tcccactggg taaaacgtgc gagtgcagac ttacaaaaat gcggattctg gctcagataa
    45661 gcgcctggtt cgacataaag gttggtaaag ttgcagcgcc caccgccaga cataaggatt
    45721 ttgcgccctg gttttttacc attatcgatc agcagaaccc ggcgtcctgc ctgacctgcc
    45781 agcgcagaac agaacatacc cgccgcacca gcgcctataa taatggcatc aaacctttcc
    45841 acgttgcgct cctcttagaa aaaacgggcg tgaattgtaa agattcctca gtggtcgcac
    45901 cagcatcaat attactaaaa ggaagtattt gcctgaatta tataagataa ttattttttg
    45961 agtgaaatcc atacaggagg caaatcaaaa aaagtctata tttcactttg cccgcgccgc
    46021 gaaagtcact gataatgcgc cgcgttcatg tcctcaaaat ggcgtaacgt cctatgctac
    46081 atttgtttgc tggcctggat ttgcataccg ggctgttatt attgcttgca ctggcttttg
    46141 tgctgttcta cgaagccatc aatggtttcc atgacacagc caacgccgtg gcaaccgtta
    46201 tctatacccg cgcgatgcgt tctcagctcg ccgtggttat ggcggcggtg ttcaactttt
    46261 tgggtgtttt gctgggtggt ctgagtgttg cctatgccat tgtgcatatg ctgccgacgg
    46321 atctgctgct taatatggga tcgtctcatg gccttgccat ggtgttctct atgttgctgg
    46381 cggcgattat ctggaacctg ggtacctggt actttggttt acctgcatcc agctctcata
    46441 cgctgattgg cgcgatcatc gggattggtt taaccaatgc gttgatgacc gggacgtcag
    46501 tggtggatgc actcaatatc ccgaaagtat taagtatttt cggttctctg atcgtttccc
    46561 ctattgtcgg cctggtgttt gctggcggtc tgattttctt gctgcgtcgc tactggagcg
    46621 gcaccaagaa acgcgcccgt atccacctga ccccagcgga gcgtgaaaag aaagacggca
    46681 agaaaaagcc gccgttctgg acacgtatcg cgctgatcct ttccgctatc ggcgtggcgt
    46741 tttcgcacgg cgcgaacgat ggtcagaaag gcattggtct ggttatgttg gtattgattg
    46801 gcgtcgcgcc agcaggcttc gtggtgaaca tgaatgccac tggctacgaa atcacccgta
    46861 cccgtgatgc catcaacaac gtcgaagctt actttgagca gcatcctgcg ctgctcaaac
    46921 aggctaccgg tgctgatcag ttagtaccgg ctccggaagc tggcgcaacg caacctgcgg
    46981 agttccactg ccatccgtcg aataccatta acgcgctcaa ccgcctgaaa ggtatgttga
    47041 ccaccgatgt ggaaagctac gacaagctgt cgcttgatca acgtagccag atgcgccgca
    47101 ttatgctgtg cgtttctgac actatcgaca aagtggtgaa gatgcctggc gtgagtgctg
    47161 acgatcagcg cctgttgaag aaactgaagt ccgacatgct tagcaccatc gagtatgcac
    47221 cggtgtggat catcatggcg gtcgcgctgg cgttaggtat cggtacgatg attggctggc
    47281 gccgtgtggc aacgactatc ggtgagaaaa tcggtaagaa aggcatgacc tacgctcagg
    47341 ggatgtctgc ccagatgacg gcggcagtgt ctatcggcct ggcgagttat accgggatgc
    47401 cggtttccac tactcacgta ctctcctctt ctgtcgcggg gacgatggtg gtagatggcg
    47461 gcggcttaca gcgtaaaacc gtaaccagta ttctgatggc ctgggtgttt acccttccgg
    47521 ctgcggtact gctttccggc gggctgtact ggctctcctt gcagttcctg taatcgtacg
    47581 caccaaaacg agcgggtcag ctggcccgct tcagattgtg acatagtgcg ctttgttcat
    47641 gccggatgcg gcgtgaacgc cttatccggc ctacaaaaac atgcaaattc aatagattgc
    47701 agagattatg taggcctgat aagcgtagcg catcaggcaa ttttgcgttt gtaatcagtc
    47761 ttgagcgggt cagtttactg gcccgctttt tttatgcgtt attattgctg aagtgcttaa
    47821 tgccaaatca tcaatgcaat caggctgacc accaccagac cacacaatgc gctggtcaga
    47881 ataaactgcc gacgcacccg ctcacagcgg cgaataaatt catcatcgtg atgatcgcga
    47941 taacgttggg catagatata ccaaacgaga cgcacctgtt tgttgggttg gccatgtgag
    48001 gtaaaaaagc cccctccatc aacatattga tagagcaatg gatcgcagtt acgcagtacc
    48061 actaacaacg cgcgtagtga tgagaaatag cgcgccatgt taacaatgca aacgacacat
    48121 aaagcccaaa ataatgcgac ggtgcttatc atacctcctc cccggcgacc tgcccgcgga
    48181 gttccacccc ggggctaccg ctcccgatac gctgccaatc agttaacacc aggtcctgga
    48241 gaaaccgctt ttgtggtgac caacatccga gcggctctat agatagtgta ggagatcagg
    48301 ttgttttttt tccagaaggt taaccactat caatatattc atgtcgaaaa tttgtttatc
    48361 taacgggtaa gcaaggcgga ttgacggatc atccgggtcg ctataaggta aggatggtct
    48421 taacactgaa tctttacggc tgggttagcc ccgcgcacgt agttcgcagg acgcgggtga
    48481 cgtaacggca caagaaacgc tagctggcca gtcatcgaca actttatgga aggagtaaca
    48541 ctatggctta taaacacatt ctcatcgcgg tcgatctctc cccggaaagc aaagttctgg
    48601 tagagaaagc agtctctatg gctcgcccct acaatgcgaa agtttctctg atccacgtag
    48661 atgtaaacta ctctgaccta tacaccgggc ttattgatgt gaatctgggt gatatgcaga
    48721 aacgcatctc tgaagagaca catcatgcgc tgaccgagct ttccactaat gcaggctacc
    48781 caatcactga aaccctgagc ggcagcggcg acctgggcca ggttctggtc gatgcaatca
    48841 agaaatacga tatggatctg gtggtttgtg gtcaccacca ggacttctgg agcaaactaa
    48901 tgtcttccgc acgtcagctg atcaacaccg ttcacgttga tatgctgatt gttccgctgc
    48961 gcgacgaaga agaataatca tccccccacg acgtgttcct gaacgcccgc atatgcgggc
    49021 gttttgcttt ttggcgcgcc ttgttacctg atgcagcgta aaccccttat ctggcctacg
    49081 atctgcgtac gcaatcaaaa tctcctgcca atacaacatt taacactatc atattttcca
    49141 tcattagtgt gatcagctgg ttattttctg ttgtaatagt gtattaatct attcaccgca
    49201 tcaatattaa gaatctctga cagatgtaaa ctttttcgcg cgttatccct tacgcgttca
    49261 tacttttcag gatggtattg gaaggttaat aaatatgaat acaacaacac ccatggggat
    49321 gctgcagcaa cctcgcccat ttttcatgat cttttttgtc gagttatggg agcgattcgg
    49381 ctactacggc gtgcagggcg tactggcggt tttcttcgtt aaacagcttg gattctcgca
    49441 agagcaggct tttgtcactt ttggtgcttt tgctgcgctg gtctatggcc tcatttccat
    49501 tggcggctat gtcggcgacc acctgctggg gaccaaacgc accattgttc tcggagcact
    49561 tgtgctggcg attggctact tcatgaccgg catgtcgcta cttaagcctg acctgatttt
    49621 catcgccctg gggactatcg ctgtcggtaa cggcctgttt aaagctaacc cagccagctt
    49681 gctttcgaag tgctatccgc cgaaagatcc gcggcttgat ggcgcattca ccctgttcta
    49741 tatgtcgatc aatatcggct cgttgatagc gttatcgctg gcccctgtga tcgctgatag
    49801 atacggttat tcagtcacct acaacctgtg cggggcgggg ttaattatcg cattactggt
    49861 ttacatcgcc tgtcgtggaa tggtgaaaga cattggttct gaacccgact tcaagccgat
    49921 gagcttcagc aaactgttgt acgtattact tggcagcgtg gtgatgatct tcgtatgtgc
    49981 atggctgatg cacaacgtag aagtcgccaa tctggtgctg attgttctct ccatcgtcgt
    50041 caccatcatc ttctttcgtc aggcattcaa gctggataaa accgggcgca ataaaatgtt
    50101 tgtcgccttt gtcctgatgc tcgaagcggt ggtgttttac attctctacg cccagatgcc
    50161 aacgtcgctg aacttctttg ccatcaacaa cgtgcatcat gaaattctcg gcttttccat
    50221 aaacccggtc agcttccagg cgcttaaccc gttctgggtg gtactcgcca gcccaatact
    50281 ggcaggcatt tacacgcatc tgggtaacaa aggcaaagac ctctcgatgc cgatgaaatt
    50341 tactctcggc atgtttatgt gctcactggg ctttttgacg gcggcagctg cgggaatgtg
    50401 gtttgcggat gcacaagggc tgacatcgcc atggtttatc gtgctggtgt atttattcca
    50461 gagcctgggt gaattgttta ttagcgccct tggcctggcg atgattgctg ccctggtgcc
    50521 gcagcatttg atgggcttta ttctcgggat gtggttcctg acgcaggctg ccgcattctt
    50581 gctgggcggc tatgtggcaa catttaccgc agtgccggac aacattaccg atccgcttga
    50641 gacgttaccc gtctatacca acgtgtttgg taagattggc ctggttacgc tgggcgttgc
    50701 agtagtgatg ctgttgatgg tgccgtggct gaaacgcatg attgcgacgc cggaaagcca
    50761 ttaattattc ttgcagaaag cagggtagcg ttatcgctac cctgctttac ttttacacgg
    50821 gcgtacctgc atagatatca aagcgatgcc ctttagtcac aaccgcgttt ggcgtggcga
    50881 cattcgctag cggtggcgcg tagtccgggc gctttactac cacgcgtttg gtcgccagca
    50941 agcgtgcagg ctccagcaat ccatcggcat caagatccgg ccccaccagc gactgaaaga
    51001 cacgcatctc ttttttcacc agcgcgcttt tctgcttatg cgggaacatc gggtcgagat
    51061 agaccacctg cgggcgtggg gtaatatcgg ttagcgccgt caggctggag gcgtgaatta
    51121 actgcaaacg ctcctgcaac cagccgccga tttccgcatc cgcataacca cgcgccaggc
    51181 cgtcgtcgag cagcgcggca acaactggat tacgctccag catccgcacg cggcaaccga
    51241 ctgaagccag cacaaaggca tcgcgcccca gtcctgcagt ggcatccacc acatccggca
    51301 aataatcgcc tttaatgccc accgctttcg ccaccgcctc accgcgacca ccgccgaatt
    51361 tgcgtcggtg cgccatcgct ccgccaacaa aatcaacaaa gatgccgcca agttttggct
    51421 catcacgctt gcgcaattcc agatgttccg gcgttaacac cagcgccatc aggttgtctt
    51481 catcgtgctc cagcccccag cgggccgcca gaacagataa ggcgccgtct ccggcgcctg
    51541 tttcatcaat taagcagatt ttcactgaat gatcagccct taatgccgta atgctccagc
    51601 atcgcatcca gctgcggttc acgaccacgg aagcgtttga acagatccat cggctcttct
    51661 gaaccgccac ggctcagaat gttgtcgagg aacgactgcc cggtttcacg gttgaaaatg
    51721 ccctcttcct caaagcgcga gaaagcatct gccgccagta cgtcagccca caggtagctg
    51781 tagtaacctg ccgcataacc accggcgaaa atatggctga aagcgtgcgg gaaacggccc
    51841 caggacggag atggcaccac ggcaaccagt ttcttgattt ctgccagagt ttcgaggatt
    51901 tttgccccct gatccgggcg gaactcggca tgaaggcgga aatcaaacag gccgaactcc
    51961 agctgacgca gaataaacag cgccgcctgg tagttcttcg ccgccagcat tttatccagc
    52021 aactctttcg gcagcggttc gccggtttca tagtgaccag agataaacgc cagcgcctcc
    52081 ggctcccagc accagttttc cataaactga ctcggcagtt cgaccgcatc ccacggcaca
    52141 ccgctgatac cggaaacacc agcggtttcg atgcgggtca gcatatggtg caggccgtga
    52201 ccgaactcgt ggaacagggt gatcacttcg tcgtgagtga acagcgccgg tttaccattt
    52261 accgggcggt tgaagttaca agtcaaatac gcgaccggtt tttgcagaga accatcagct
    52321 ttacgcatct ggcctacgca gtcatccatc cacgccccgc cgcgcttgtt ttcacgggca
    52381 tacagatcga ggtagaagct gccgcgcagt tcgttatttt cgtcatacag ttcgaagaaa
    52441 cgtacatccg gatgccagac atcaacctct ttacgctctt tagcggtgat gccgtaaata
    52501 cgtttcacca cttcaaacag gccgttaacc gctttgtttt ccgggaagta cggacgcagc
    52561 tgctcgtcgc tgatgctgta gaggtgctgt ttctgttttt cgctgtagta agcgatatcc
    52621 cacggctgca actcatcgac accaaattcg gctttagcga aggcacgcag ttgctccagc
    52681 tctttttcgc cttgcggacg cgcgcgtttt gccagatcgg ttaagaaatc cagcacctgc
    52741 tgcgggtttt ctgccatttt agtggcaagg gatttaaagg cgtagttttc aaagcccagc
    52801 agttgcgcca gttcgtgacg cagcgcgagg atctcttcca tcaccttgct gttatcccac
    52861 ttaccggcgt tcgggccttg atcggaggcg cgggtgctgt aagcgcgata catctcttcg
    52921 cgcagagcct ggttgtcgca gtaggtcatt accggcaggt agcttgggat atccagcgtc
    52981 agcaggtagc cttccagctc tttcgcttcg gcctgggctt ttgccgcagc cagcgcgctt
    53041 tctggcatcc ccgccagccc cgcttcgtcg gtaacgagtt tggtccagcc catcgtcgca
    53101 tcgaggacgt tgttgctgta ctggttgccc agttcagaaa gacgggtagc aatttcgccg
    53161 taacgctgct gtttctcttt cggcagacct atgccagaga gttcaaaatc acgcagggcg
    53221 ttatcaaccg ctttcttctg cgccgtgttc agcgtggcgt aatgatcgcc atcgcgcagg
    53281 tcgcggtacg ctttatacag cccttcatgt tgccctaccc aggtgctgta ttccgacagc
    53341 agcggcaggg tttgctcgta ggcttcacgc agttccgggc tatttttcac cgagttcagg
    53401 tggctgaccg gggagaagat acgccccaac acatcgtcca cttccgccaa cggctggcag
    53461 agattttccc aggtgtacgg tgccccttgc gctactacgc gcgccacatt ttcgcggcaa
    53521 tcgttcagcg cctgagtcac ggctggaacg acatgttccg ggagaatttt agaaaacgga
    53581 ggcaattcaa agggagtcag taacggattc gtcattcgcg cagtcctggt taaagaggtt
    53641 aaggaagcgc tcaacaggcg ctttacataa tgtgtgtagc atggggttaa gtgtagtgaa
    53701 tttcaatgag aaacgttacg ctttcgcggc ggcggcctct tttcggtata ctgtcctgat
    53761 acgcttttgt gcgccccgaa tacgggccga tttttactta ccggaacacc tttacccatg
    53821 ctcagttatc gccacagctt tcacgctggc aaccacgccg acgtccttaa acataccgtt
    53881 cagagcctga tcatcgagtc gctgaaagag aaagataaac cgtttctcta tctcgatacc
    53941 cacgcagggg ctgggcgtta tcagttaggc agcgaacatg ccgagcgtac cggcgaatat
    54001 ctcgaaggta tcgcccgtat ctggcagcag gacgatttgc ccgcagaact ggaggcgtac
    54061 atcaatgtgg taaaacactt caaccgtagc ggtcagttgc gttactaccc cgggtcgccg
    54121 ttgattgctc gccagctact gcgcgaacaa gacagcctgc aactgaccga actgcacccg
    54181 agcgattacc cgttgctgcg ttctgaattt cagaaagaca gccgtgcgcg cgtcgaaaaa
    54241 gccgacggtt tccagcagct taaggccaaa cttccgccgg tttcccgccg tggtttaatc
    54301 cttatcgacc cgccgtatga aatgaaaacc gactatcagg cggtggtcag cgggatagca
    54361 gaaggttaca aacgtttcgc cactggtact tacgcactgt ggtatccggt ggtgctgcgt
    54421 cagcaaatta agcgcatgat ccacgatctg gaagcgaccg gtattcgcaa aattctgcaa
    54481 attgaactgg cggtactgcc agacagcgat cgccgtggca tgaccgcttc cggcatgatt
    54541 gtgattaacc cgccgtggaa actggaacag cagatgaata acgtgctgcc gtggctgcac
    54601 agcaaactgg ttccggcagg caccgggcac gccaccgtaa gctggatcgt gccggagtaa
    54661 ttgcagccat tgctggcacc tattacgtct cgcgctacaa tcgcggtaat caacgataag
    54721 gacactctgt catgactaaa cactatgatt acatcgccat cggcggcggc agcggcggta
    54781 tcgcctccat caaccgcgcg gctatgtacg gccagaaatg cgcactgatt gaagccaaag
    54841 agctgggcgg cacctgcgtg aacgtcggct gcgtgccgaa aaaagtgatg tggcacgcgg
    54901 cgcaaatccg tgaagcgatc catatgtacg gccctgatta tggtttcgat accaccatca
    54961 acaagttcaa ctgggaaacg ttgatcgcca gccgtaccgc ctatatcgac cgtattcata
    55021 cttcctatga aaacgtgctc ggtaaaaata acgttgatgt aatcaaaggc tttgcccgct
    55081 tcgttgatgc caaaacgctg gaggtaaacg gcgaaaccat cacggccgat catattctga
    55141 tcgccacagg cggtcgtccg agccacccgg atattccggg cgtggaatac ggtattgatt
    55201 ctgatggctt cttcgccctt cctgctttgc cagagcgcgt ggcggttgtt ggcgcgggtt
    55261 acatcgccgt tgagctggcg ggcgtgatta acggcctcgg cgcgaaaacg catctgtttg
    55321 tgcgtaaaca tgcgccgctg cgcagcttcg acccgatgat ttccgaaacg ctggtcgaag
    55381 tgatgaacgc cgaaggcccg cagctgcaca ccaacgccat cccgaaagcg gtagtgaaaa
    55441 atgccgatgg tagcctgacg ctggagctgg aagatggtcg cagtgaaacg gtggattgcc
    55501 tgatttgggc gattggtcgc gagcctgcca atgacaacat caacctagaa gccgctggcg
    55561 ttaaaactaa cgaaaaaggc tatatcgtcg tcgataaata tcaaaacacc aatattgaag
    55621 gtatttacgc ggtgggcgat aacacgggtg cagtggaact gacaccggta gcagttgcag
    55681 cgggtcgccg tctctctgaa cgcctgttta ataacaagcc ggatgagcat ctggattaca
    55741 gcaacattcc gaccgtggtc ttcagccatc cgccgattgg tactgttggt ttaacggaac
    55801 cgcaggcgcg cgagcagtac ggcgacgatc aggtgaaagt gtataaatcc tctttcaccg
    55861 cgatgtatac cgccgtcacc actcaccgcc agccgtgccg catgaagctg gtgtgcgttg
    55921 ggccagaaga gaagattgtc ggtattcacg gcattggctt tggtatggac gaaatgttgc
    55981 agggcttcgc ggtggcgctg aagatgggcg caaccaaaaa agacttcgac aataccgtcg
    56041 ccattcaccc aacggcggca gaagagttcg tgaccatgcg ctaaatgtta aagggctaag
    56101 agtagtgtgc tcttagccct taattacgtt tccgctatca gttcagaagc tgaagcagaa
    56161 agcggatcag ttccagcagc gcaattaacg ctccaagaac gatgattgct ttatcaatca
    56221 cccgttttct ccatgcgatg gagtgagaat gcatccgctt actcatccac tgcctgtcac
    56281 ggcgcatgtc tcattgttag atgacgacta tctcactccg gccagagcat cagttaacgg
    56341 caccacccgt acttctgacc aggactttga aagcgtttat gcgcattacc agagtgaaaa
    56401 tgcctcagag ctaactggat aatcatacag tacattcagg ttataaaacc agcatgtcct
    56461 tgcaatagtt tcagtatggt attagcattg atgcgttaga tgatgactat ctcactccag
    56521 ccagagccac caactcaggg ctgaagagta aaaaaaccga cgcaaagtcg gtttttttac
    56581 atccggattc ggacaaggct taatatgacg atgacccagt gaaagtatat aaatcgtcat
    56641 tgcgatatat accgaagtgc cccctccgcc agctgaagaa atcgctaatt cttgcaatgt
    56701 tagccactgg ctaatagtat tgaaccgtta gatgtgggca ttctcactcc agccagagcc
    56761 accaactcag gctgaagagt aaaaaaccga cgcaaagtcg gtttttttac gtcctgattc
    56821 aaaccgcctt tcaaatgaat agccaactca aaattcacac ctattacctt cctctgcact
    56881 tacacattcg ttaagtcata tatgtttttg acttatccgc ttcgaagaga gacactacct
    56941 gccacaatca ggagcgcaat atgtcatttc tgttacccat ccaattgttc aaaattcttg
    57001 ctgatgaaac ccgtctgggc atcgttttac tgctcagcga gctgggagag ttatgcgtct
    57061 gcgatctttg tactgccctc gatcagtcgc agcccaagat ctcccgccac ctggcattgc
    57121 ttcgtgaaag cgggctattg ctggaccgca agcaaggtaa gtgggttcat taccgcttat
    57181 caccgcatat tccagcatgg gcggcgaaaa ttattgatga ggcctggcga tgtgaacagg
    57241 aaaaggttca ggcgattgtc cgcaacctgg ctcgacaaaa ctgttccggg gacagtaaga
    57301 acatttgcag ttaaaaattt agctaaacac atatgaattt tcagatgtgt tttatccggg
    57361 aggcattatg ttactggcag gcgctatctt tgtcctgacc atcgtattgg ttatctggca
    57421 gccgaaaggt ttaggcatcg gctggagtgc aacgctcggc gcagtactgg cgttagttac
    57481 gggcgtggtt catccgggtg atattccggt ggtgtggaat atcgtctgga acgcgacggc
    57541 tgcgtttatc gccgtcatta tcatcagcct gctgctggat gagtccggct tttttgaatg
    57601 ggcggcgctg cacgtctcac gctggggtaa tggtcgtggt cgcttgctgt ttacctggat
    57661 tgtcctgctc ggtgctgccg ttgccgccct gtttgccaat gatggcgcgg cgcttatttt
    57721 gacgccgatt gtcatcgcca tgctgctggc tttaggattc agtaaaggca cgacgctggc
    57781 gttcgtgatg gcggccggat tcattgccga taccgccagc ctgccgctta ttgtctccaa
    57841 cctggtgaat atcgtttccg ctgatttctt tggcctcggc tttcgcgaat acgcctcggt
    57901 gatggtgccg gtggatatcg ccgcgattgt tgccacgctg gtgatgctac atctctattt
    57961 tcgcaaagat attccgcaga actacgatat ggcgctgctg aaatctcccg cagaagcgat
    58021 caaagatcct gctacgttca aaactggctg ggttgtttta ctgcttctgc tggtgggatt
    58081 tttcgtcctg gaaccgctcg gcattccggt gagcgccatt gcagctgtgg gcgcgctgat
    58141 attatttgtc gtcgctaaac gcggtcatgc gattaatacg ggtaaagtcc tgcgcggtgc
    58201 cccctggcag attgtcatct tctcgctcgg catgtatctg gtggtttatg gcctgcgcaa
    58261 tgccggatta acggaatatc tttctggcgt actcaacgtg ctggcggata acggcctgtg
    58321 ggccgcgacg ctcggcaccg gattcctcac cgccttcctc tcttctatta tgaacaatat
    58381 gccgacggta ctggttggcg cgttgtccat tgatggcagc acggcatctg gcgttatcaa
    58441 agaagcgatg gtttatgcca atgtgattgg ctgcgatttg ggaccgaaaa ttaccccaat
    58501 tggtagcctg gctacgctac tctggctgca cgtactttcg cagaagaata tgactatcag
    58561 ctggggatat tacttccgta cagggattat catgaccctg cctgtgctgt ttgtgacgct
    58621 ggctgcgctg gcgctacgtc tctctttcac tttgtaatga gatactgata tgagcaacat
    58681 taccatttat cacaacccgg cctgcggcac gtcgcgtaat acgctggaga tgatccgcaa
    58741 cagcggcaca gaaccgacca ttatccatta tctggaaact ccgccaacgc gcgatgaact
    58801 ggtcaaactc attgccgata tggggatttc cgtacgcgcg ctgctgcgta aaaacgtcga
    58861 accgtatgag gagctgggcc ttgcggaaga taaatttact gacgatcggt taatcgactt
    58921 tatgcttcag cacccgattc tgattaatcg cccgattgtg gtgacgccgc tgggaactcg
    58981 cctgtgccgc ccttcagaag tggtgctgga aattctgcca gatgcgcaaa aaggcgcatt
    59041 ctccaaggaa gatggcgaga aagtggttga tgaagcgggt aagcgcctga aataaagcgg
    59101 cgatatccac tgccgatagt tggtaaagtg cgctttgttt atgtcggatg cgacgctaac
    59161 gcgtcttatc cgacctacaa aaccgtgcaa attcaatata tggcatgaaa tatgtagagc
    59221 gtagcgcatc aggcaatttt gctatttacg cagcactcct cgcgtaagaa ttcaattatc
    59281 gcttccagca ttgcgtattg cgaaacacac atcagcgtcc gactctcacg gttttgtttg
    59341 accagtccta ccgataccag tgctgatata tggtggctga gtgtggagcc aggtatccca
    59401 agctgctttt gcaactcacc gaccggcaac ccttgttccc cggctttgac aagatgttta
    59461 aatatgaaca aacgggtcgg gtgacctagc tctttcagcg ctttagcgac ttcttccaac
    59521 tgcatatgac acccctctgt ctggttgata tttcgataat accagaaata tcttgatctg
    59581 aatttcattt cgattattct ggaaatatag atttatccaa tgaggttacg tatgagtagt
    59641 tggcttgcga tgctgcaaga tgccgcagag atgtttgtgt ttctcgccgt cgagctttcg
    59701 ttgctgttta tagtgattag tgccggtgtc agcctgataa gacaaaaggt gccagaccat
    59761 aaaatccagc agatgatggg ggccagaaaa gggagaggct atctcctggc tgctctgttg
    59821 ggagccgtta ccccgttctg tagttgctcg acaataccca tgttacgtgg attgttgtca
    59881 gcgaaagccg ggt
//
LOCUS       DAZPLI010000007        59046 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZPLI010000007 DAZPLI010000000
VERSION     DAZPLI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.007"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(78..1025)
                     /gene="dusC"
                     /locus_tag="ACHUU3_000389"
     CDS             complement(78..1025)
                     /gene="dusC"
                     /locus_tag="ACHUU3_000389"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR2080566.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKA
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            1264..1662
                     /locus_tag="ACHUU3_000390"
     CDS             1264..1662
                     /locus_tag="ACHUU3_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR2080567.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            1659..2354
                     /locus_tag="ACHUU3_000391"
     CDS             1659..2354
                     /locus_tag="ACHUU3_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR2080568.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            2484..3368
                     /gene="cdd"
                     /locus_tag="ACHUU3_000392"
     CDS             2484..3368
                     /gene="cdd"
                     /locus_tag="ACHUU3_000392"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR2080569.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            3518..4237
                     /gene="sanA"
                     /locus_tag="ACHUU3_000393"
     CDS             3518..4237
                     /gene="sanA"
                     /locus_tag="ACHUU3_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR2080570.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            4240..4479
                     /locus_tag="ACHUU3_000394"
     CDS             4240..4479
                     /locus_tag="ACHUU3_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR2080571.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            4673..5911
                     /gene="preT"
                     /locus_tag="ACHUU3_000395"
                     /pseudo
     CDS             4673..5911
                     /gene="preT"
                     /locus_tag="ACHUU3_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            5905..7140
                     /gene="preA"
                     /locus_tag="ACHUU3_000396"
     CDS             5905..7140
                     /gene="preA"
                     /locus_tag="ACHUU3_000396"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFR2080572.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(7267..8277)
                     /gene="mglC"
                     /locus_tag="ACHUU3_000397"
     CDS             complement(7267..8277)
                     /gene="mglC"
                     /locus_tag="ACHUU3_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR2080573.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8293..9813)
                     /gene="mglA"
                     /locus_tag="ACHUU3_000398"
     CDS             complement(8293..9813)
                     /gene="mglA"
                     /locus_tag="ACHUU3_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR2080574.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKFRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(9874..10872)
                     /gene="mglB"
                     /locus_tag="ACHUU3_000399"
     CDS             complement(9874..10872)
                     /gene="mglB"
                     /locus_tag="ACHUU3_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR2080575.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(11152..12150)
                     /gene="galS"
                     /locus_tag="ACHUU3_000400"
     CDS             complement(11152..12150)
                     /gene="galS"
                     /locus_tag="ACHUU3_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR2080576.1"
                     /translation="MATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQV
                     SDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRC
                     NALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNN
                     GHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELL
                     GRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRY
                     PIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAITNSTNQAM"
     gene            complement(12334..13491)
                     /gene="yeiB"
                     /locus_tag="ACHUU3_000401"
     CDS             complement(12334..13491)
                     /gene="yeiB"
                     /locus_tag="ACHUU3_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR2080577.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(13508..14176)
                     /gene="folE"
                     /locus_tag="ACHUU3_000402"
     CDS             complement(13508..14176)
                     /gene="folE"
                     /locus_tag="ACHUU3_000402"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR2080578.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            14434..15270
                     /gene="yeiG"
                     /locus_tag="ACHUU3_000403"
     CDS             14434..15270
                     /gene="yeiG"
                     /locus_tag="ACHUU3_000403"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR2080579.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(15302..17293)
                     /gene="cirA"
                     /locus_tag="ACHUU3_000404"
     CDS             complement(15302..17293)
                     /gene="cirA"
                     /locus_tag="ACHUU3_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFR2080580.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(17585..19054)
                     /gene="lysP"
                     /locus_tag="ACHUU3_000405"
     CDS             complement(17585..19054)
                     /gene="lysP"
                     /locus_tag="ACHUU3_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR2080581.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(19259..20140)
                     /gene="yieE"
                     /locus_tag="ACHUU3_000406"
     CDS             complement(19259..20140)
                     /gene="yieE"
                     /locus_tag="ACHUU3_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR2080582.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20239..21288
                     /locus_tag="ACHUU3_000407"
     CDS             20239..21288
                     /locus_tag="ACHUU3_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR2080583.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            21362..22219
                     /gene="nfo"
                     /locus_tag="ACHUU3_000408"
     CDS             21362..22219
                     /gene="nfo"
                     /locus_tag="ACHUU3_000408"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR2080584.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            22223..23311
                     /locus_tag="ACHUU3_000409"
     CDS             22223..23311
                     /locus_tag="ACHUU3_000409"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR2080585.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(23367..24617)
                     /locus_tag="ACHUU3_000410"
     CDS             complement(23367..24617)
                     /locus_tag="ACHUU3_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR2080586.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(24717..25658)
                     /gene="rihB"
                     /locus_tag="ACHUU3_000411"
     CDS             complement(24717..25658)
                     /gene="rihB"
                     /locus_tag="ACHUU3_000411"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR2080587.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            25788..26486
                     /locus_tag="ACHUU3_000412"
     CDS             25788..26486
                     /locus_tag="ACHUU3_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFR2080588.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(26553..27803)
                     /locus_tag="ACHUU3_000413"
     CDS             complement(26553..27803)
                     /locus_tag="ACHUU3_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR2080589.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(27897..28835)
                     /gene="psuG"
                     /locus_tag="ACHUU3_000414"
     CDS             complement(27897..28835)
                     /gene="psuG"
                     /locus_tag="ACHUU3_000414"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR2080590.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(28823..29764)
                     /locus_tag="ACHUU3_000415"
     CDS             complement(28823..29764)
                     /locus_tag="ACHUU3_000415"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR2080591.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(30222..31913)
                     /gene="fruA"
                     /locus_tag="ACHUU3_000416"
     CDS             complement(30222..31913)
                     /gene="fruA"
                     /locus_tag="ACHUU3_000416"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR2080592.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(31930..32868)
                     /gene="fruK"
                     /locus_tag="ACHUU3_000417"
     CDS             complement(31930..32868)
                     /gene="fruK"
                     /locus_tag="ACHUU3_000417"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR2080593.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(32868..33998)
                     /gene="fruB"
                     /locus_tag="ACHUU3_000418"
     CDS             complement(32868..33998)
                     /gene="fruB"
                     /locus_tag="ACHUU3_000418"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR2080594.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            34366..35547
                     /gene="setB"
                     /locus_tag="ACHUU3_000419"
     CDS             34366..35547
                     /gene="setB"
                     /locus_tag="ACHUU3_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR2080595.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(35544..35798)
                     /locus_tag="ACHUU3_000420"
     CDS             complement(35544..35798)
                     /locus_tag="ACHUU3_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR2080596.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            35953..36525
                     /gene="yeiP"
                     /locus_tag="ACHUU3_000421"
     CDS             35953..36525
                     /gene="yeiP"
                     /locus_tag="ACHUU3_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR2080597.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            36748..38214
                     /locus_tag="ACHUU3_000422"
     CDS             36748..38214
                     /locus_tag="ACHUU3_000422"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR2080598.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            38332..39318
                     /gene="yeiR"
                     /locus_tag="ACHUU3_000423"
     CDS             38332..39318
                     /gene="yeiR"
                     /locus_tag="ACHUU3_000423"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR2080599.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            39357..40070
                     /gene="lpxT"
                     /locus_tag="ACHUU3_000424"
     CDS             39357..40070
                     /gene="lpxT"
                     /locus_tag="ACHUU3_000424"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR2080600.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            40482..41048
                     /gene="mepS"
                     /locus_tag="ACHUU3_000425"
     CDS             40482..41048
                     /gene="mepS"
                     /locus_tag="ACHUU3_000425"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR2080601.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            41229..42785
                     /locus_tag="ACHUU3_000426"
     CDS             41229..42785
                     /locus_tag="ACHUU3_000426"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR2080602.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            42867..44681
                     /locus_tag="ACHUU3_000427"
     CDS             42867..44681
                     /locus_tag="ACHUU3_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR2080603.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            44682..45776
                     /locus_tag="ACHUU3_000428"
     CDS             44682..45776
                     /locus_tag="ACHUU3_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR2080604.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            45776..46801
                     /locus_tag="ACHUU3_000429"
     CDS             45776..46801
                     /locus_tag="ACHUU3_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR2080605.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            46803..48392
                     /gene="yejF"
                     /locus_tag="ACHUU3_000430"
     CDS             46803..48392
                     /gene="yejF"
                     /locus_tag="ACHUU3_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR2080606.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(48396..48740)
                     /locus_tag="ACHUU3_000431"
     CDS             complement(48396..48740)
                     /locus_tag="ACHUU3_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR2080607.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(49073..50130)
                     /locus_tag="ACHUU3_000432"
                     /pseudo
     CDS             complement(49073..50130)
                     /locus_tag="ACHUU3_000432"
                     /inference="COORDINATES: protein motif:HMM:NF008314.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(50221..50916)
                     /gene="rsuA"
                     /locus_tag="ACHUU3_000433"
     CDS             complement(50221..50916)
                     /gene="rsuA"
                     /locus_tag="ACHUU3_000433"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR2080608.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            51066..52826
                     /locus_tag="ACHUU3_000434"
     CDS             51066..52826
                     /locus_tag="ACHUU3_000434"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR2080609.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            52951..53235
                     /gene="rplY"
                     /locus_tag="ACHUU3_000435"
     CDS             52951..53235
                     /gene="rplY"
                     /locus_tag="ACHUU3_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR2080610.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(53374..54381)
                     /gene="yejK"
                     /locus_tag="ACHUU3_000436"
     CDS             complement(53374..54381)
                     /gene="yejK"
                     /locus_tag="ACHUU3_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR2080611.1"
                     /translation="MGLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            54563..54790
                     /locus_tag="ACHUU3_000437"
     CDS             54563..54790
                     /locus_tag="ACHUU3_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR2080612.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            54810..56570
                     /gene="yejM"
                     /locus_tag="ACHUU3_000438"
     CDS             54810..56570
                     /gene="yejM"
                     /locus_tag="ACHUU3_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR2080613.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            56645..56721
                     /locus_tag="ACHUU3_000439"
     tRNA            56645..56721
                     /locus_tag="ACHUU3_000439"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56679..56681,aa:Pro,seq:ggg)
     gene            complement(56824..59046)
                     /gene="yejO"
                     /locus_tag="ACHUU3_000440"
                     /pseudo
     CDS             complement(56824..59046)
                     /gene="yejO"
                     /locus_tag="ACHUU3_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        14537 a        15406 c        14403 g        14700 t
ORIGIN      
        1 attttgacgc caccaaacaa ccgtgcggcc tattgataaa gagctaacac attgtcaaaa
       61 aacatcacta tggtttttta gagtttctcg atatcaattg cctgaatagc ccttgcaata
      121 tcaggggaat tattcaacac ccgaacatgc tgaaataatt ccgttgcttc atcgtattct
      181 ttacgcaaat aactcaacca ctgtttaatc cgcgcaacgt gatataaccc ggtatcgccc
      241 tgcttttcca gacgggtata tttttgcagc aaagcaacca cctccggcca cggcattcgc
      301 ggttcgttat attttactac ccggctcagg ttgggaatat tgagcgcccc gcgaccaatc
      361 atcactgcgt cgcagccgct gatcgcaatg cattgttgcg cgctctgcca gtcccagatt
      421 tcgccgttgg caatcaccgg aatattcagc cgctggcgaa tctcgccaat cgcctgccag
      481 tcaatatgct ccgcgcggta accctgctct tttgtccgtc catgcaccac cagctccgta
      541 gcaccagcct gttgaaccgc atcggcgatt tcaaatttct tctcaccgct gtcccagccc
      601 agacgcactt tcacgctgac gggcaaatgc gccggtacag cttcacgcat cgcttttgca
      661 ccctggtaga tgagttcagg atctttgagt aacgtcgccc cgccgccgct gccgttaacc
      721 gctttcgacg ggcagccgca attgagatcc acgccccagg aacctaattc caccgcacgg
      781 gcggcgttct ctgccagcca ttgtgggaac tgacctaaca actgaacgcg caccagcgta
      841 ccggatggtg tccggctggc gttttgtagc tcagggcaaa tgcgatgaaa gacttttacc
      901 ggcagcagtt gatccaccac gcggacaaac tcggtgatgc acagatcgta gtcgttaact
      961 tcggtcagca attctcgcac cagagagtca agcacaccct ccatcggagc cagtaacaca
     1021 cgcatatctt cacccggaaa aaaatgaggc gctatgttag cgcctatggt caacggatta
     1081 aaggtctgca aagagggatt gctgcaaact gtaatccctc tctatgcttg atgtatgcct
     1141 gagttcgttg cgcattgcgt aatgccgggg agcgaaaaaa taagcgctga tttcttaatg
     1201 tgatcggtag cacgttttac gaattaattg tatgatgaat ccatctcatc tggggtgttg
     1261 attatgagta agacactgaa cattatctgg caatatttac gcgctttcgt cctgatttat
     1321 gcctgcctgt atgcaggcat tttcattgct tccctgctac cggtaaccat tccgggcagc
     1381 atcatcggga tgctgatcct gtttgtcctg ctggccttgc aaattcttcc ggcaaaatgg
     1441 gtcaatccgg ggtgctacgt actgattcgc tatatggcgc tattgtttgt gccgattggc
     1501 gtaggcgtca tgcaatattt tgatttgctc cgcgcacagt ttggcccggt agtggtttcc
     1561 tgtgcagtca gtacgctggt ggtttttctg gtggtgagct ggagttcaca actggtacac
     1621 ggtgaacgta aagtcgtagg tcagaaagga tcagaagaat gatggcgaat atctggtggt
     1681 cattaccgct gacgttgatt gtcttttttg ccgcccgcaa actggcggca cggtataagt
     1741 ttcccctgct taacccgttg ctggtagcaa tggtggtgat cattcctttt ttaatgctga
     1801 ccggcatctc ttacgacagc tactttaaag gtagcgaagt gctaaacgat ctgctgcaac
     1861 cggcggtcgt cgcgctggcc tatcctttat atgagcagct acaccagatc cgcgcgcgct
     1921 ggaaatcgat catcaccatc tgttttatcg gcagcgtggt tgcgatggtg acggggactt
     1981 ccgtggcatt gttgatgggc gcttcaccgg aaatcgccgc gtcaatcctg ccgaaatcag
     2041 tcaccacgcc tattgcaatg gcggttggcg gcagtattgg cggtattccg gcaatcagcg
     2101 cagtttgcgt gattttcgtc ggcatcctcg gcgcggtatt tggccataca ttgcttaatg
     2161 cgatgcgtat tcgtaccaaa gctgcgcgcg gtctggcaat ggggacagcc tcgcacgccc
     2221 tcggtacggc gcgctgcgcc gagctggatt atcaggaagg tgcatttagt tcgctggcgc
     2281 tggtgttatg cgggataatt acttcgctga tcgcaccgtt ccttttcccg attattctgg
     2341 cagtaatggg ctaaaatttg cgatgcgtcg cgcatttttg atgtaagttt cacgcgttgc
     2401 ataattaatg agattcagat cacatataaa gccataacgg gttcgtaaac tgttatccca
     2461 ttacatgatt atgaggcaac gccatgcatc cacgttttca aaccgctttt gcccaacttg
     2521 cggataactt gcaatctgca ctggaaccta ttctggcaga caagtacttc cccgctttgt
     2581 tgaccgggga gcaagtctca tcgctgaaga gcgcaacggg gctggacgaa gacgcgctgg
     2641 cattcgcact acttccactg gcggcggcct gtgcgcgtac gccattgtcg aattttaatg
     2701 ttggcgcaat tgcgcgcggt gtgagcggaa cctggtattt cggtgccaat atggaattta
     2761 ttggtgcaac aatgcagcaa accgttcatg ccgaacaaag cgcgatcagc cacgcctggt
     2821 tgagtggtga aaaagcgctt gcagccatca ccgttaacta tacgccttgt ggtcactgcc
     2881 gtcagtttat gaatgaactg aacagcggtc tggatctgcg tattcatctg ccgggccgcg
     2941 aggcacacgc gctgcgtgac tatctgccgg atgcctttgg gccgaaagat ctggagatta
     3001 aaacgctgct gatggacgaa caggatcacg gctatgcgct gacgggtgat gcgctttctc
     3061 aggcagcgat tgcggcggca aaccgttcgc acatgcctta cagtaagtcg ccaagcggtg
     3121 tcgcgctgga atgtaaagac ggtcgtattt tcagcggcag ctacgctgaa aacgccgcat
     3181 tcaacccgac tctgccaccg ttgcaggggg cgttaattct gttgaatctc aagggttatg
     3241 attacccgga tatccagcgc gcggttctgg cagaaaaagc cgatgcgccg ttgattcagt
     3301 gggatgccac ctccgcaacg ctgaaagctc tcggctgtca cagtatcgac cgcgtgcttc
     3361 tcgcttaagc ctggtgccgg atgcggcgtg aacgccttgt ccggcttgcc agccctctcc
     3421 tggtgtcgaa attcccggca aacagtttgc cgtttcttgc gcaaaaccag cgggtaaagt
     3481 agcctgatgg aaattttcct tagatcgagt ctcctgcatg ttaaagcgcg tgttcctcag
     3541 cctgttagtc ctgatcggct tgctgctgtt gactgtgctc ggcctcgatc gctggatgag
     3601 ctggaaaacc gcgccttata tctacgacga attgcaggat ctcccctacc gccaggtcgg
     3661 cgtggtgctc ggcacagcaa aatattatcg tactggcgta attaatcagt attatcgcta
     3721 ccgcattcaa ggagcgatta atgcctataa cagcggtaag gtaaattatc tattactgag
     3781 cggcgataac gcattgcaaa gttataatga gccgatgacc atgcgcaaag atttaatcgc
     3841 tgctggtgtc gacccatcag atattgttct cgattacgca ggctttcgta cgctggattc
     3901 catcgtgcgt acacgcaaag tgttcgatac taatgatttc attattatca cccaacgttt
     3961 ccactgtgag cgggcattat ttattgcgct gcatatgggg attcaggctc agtgttatgc
     4021 cgtaccgtca ccgaaagata tgctgtcagt acgtattcgc gaatttgccg cccgtttcgg
     4081 tgcgctggct gacctttata tttttagacg tgaaccgcgt tttttagggc cgctggtccc
     4141 tattccggct atgcaccagg tgccggaaga tgcgcagggg tatcccgccg tcacacccga
     4201 acagttactt gaattacaaa agaaacaagg aaagtagtta tggatgtaca gcagtttttt
     4261 gtcgttgccg tttttttcct tatcccgata ttttgtttcc gcgaagcatg gaaaggctgg
     4321 cgcgcaggcg cgattgataa acgggttaaa aatgcaccgg aaccggtgta tgtctggcga
     4381 gcaaaaaatc ccggactctt tttcgcttat atggtggcat atatcggctt cggaatttta
     4441 tctatcggca tgattgttta tcttattttc tatcgttaat attttctctt ttctgttaat
     4501 tattgagaat aattattact tcactcaata agctgcggat ataacattct gaaccgcagc
     4561 tatttgtgaa tcctttcaca gtttaaattt ccccacacgc ttagccttaa tatcagcacg
     4621 caatatttat ttagcgttcg ccttaattac tcatagcatt aaggaagatc acatgccgca
     4681 acaaaattat ctggatgaac tcactccggc ttttacgcct ttactggcga ttaaagaagc
     4741 ctctcgctgt ttattatgtc atgacgctcc ctgtagtcag gcttgcccgg cacagaccga
     4801 tccggggaaa tttattcgct caatctactt tcgtaatttt aaaggcgctg ccgagacaat
     4861 tcgcgaaaat aatgccctcg gtgccgtttg tgccagagtg tgcccgacgg agaaattatg
     4921 ccaaagcggt tgtacccgtg ccggtgttga tgctcccatt gatatcggcc gcttacagcg
     4981 ttttgttact gattttgaac aacaaaccgg gatggaaatt tatcagcccg gtactaaaac
     5041 gctcggcaaa gtcgcgatta ttggcgcagg tcctgccgga ttgcaggcca gtgtgacact
     5101 gacaaaccag ggttatgacg tcacgatcta tgagaaagaa gcacaacccg gtggctgatt
     5161 gcgtaacggc attccgcaat tccggttacc gcaatcagta ctggatgcag agatcgcccg
     5221 tattgaaaaa atgggcgtga ccattaagtg caacaacgaa gtcggtaaca cactcaccct
     5281 tgagcagctg aaagcagaaa accgcgcggt actggtcacc gtggggttat caagcggttc
     5341 cgggctaccg ctgtttgagc atagtgacgt tgagattgcc gtcgacttct tgcaacgtgc
     5401 acgacaggcg caaggcgata tcagcattcc acaaagcgca ttaattatcg gcggcggtga
     5461 tgtcgcgatg gacgtagcca gcacgctgaa agttctcggc tgtcaggcgg taacttgcgt
     5521 agcgcgtgaa gagttagatg agtttccggc aagcgaaaaa gagtttacca gcgcccggga
     5581 gctgggcgtt tcgatcatcg atggattcac gccagtagcc gtggaaggca ataaagtcac
     5641 gtttaagcat gtacggttat cgggcgaact gacgatggcg gcagataaaa ttattctcgc
     5701 cgtcggtcag catgccagac tagatgcctt tgcggagtta gagccgcagc gtaacaccat
     5761 caaaacacaa aattaccaga cccgcgaccc gcaagtcttt gctgctggcg atattgttga
     5821 gggagacaaa accgtggtct atgccgtgaa aaccgggaaa gaagccgccg aggcgattca
     5881 tcactattta gagggagctt gctcatgtta acgaaagatc tttcgattac tttttgcggc
     5941 gtgaagtttc ccaacccgtt ctgcctctct tcttcgccgg taggcaactg ctatgagatg
     6001 tgtgccaaag cctacgacac aggttggggc ggtgtggtgt ttaaaacgat cggctttttt
     6061 atcgccaacg aagtctcgcc gcgttttgat catctggtga aagaagatac cggttttatc
     6121 ggcttcaaaa atatggagca gattgccgag catccgctgg aggagaatct ggccgccctg
     6181 cgtcggctga aagaagatta cccggacaaa gtattgatcg cttcgatcat gggggaaaat
     6241 gagcagcaat gggaggagct ggcgcgcctg gtgcaagaag ctggcgcgga tatgatcgag
     6301 tgtaacttct cctgtccgca aatgacttct catgcgatgg gtagcgatgt cgggcaaagc
     6361 ccggagctgg tagaaaaata ttgtcgggca gtgaaacggg gttccacgct gccaatgctg
     6421 gcgaagatga cgccgaatat cggtgatatg tgcgaagtgg cgctggcggc gaagcgcggc
     6481 ggcgcagatg gcattgcggc gattaacacc gttaaatcca tcaccaatat cgatcttaat
     6541 cagaaaatcg gtatgccgat cgttaacgga aaatcaagta tttccggata ttccggtaaa
     6601 gcggtaaaac cgatcgccct gcgcttcatt cagcaaatgc gcacccatcc agaactgcgc
     6661 gatttcccaa tcagcggtat cggcggcatt gaaacctggg aggatgcggc ggagttttta
     6721 ttgctcggcg cagcaacgtt acaggtcacc accggcatta tgcagtacgg ctatcgcatc
     6781 gtagaagata tggcgagcgg gttatcgcat tatctcgccg atcagggatt tgattcgttg
     6841 caggagatgg taggtctggc gaataacaat attgtcccgg cggaagattt agaccgcagt
     6901 tatattgtct atccccgtat caatcttgac aaatgtgttg gctgtggacg ctgttatatt
     6961 tcctgttacg acggcggtca ccaggcgatg gaatggagcg agaaaacccg cacaccgcat
     7021 tgtaataccg agaaatgtgt gggttgtctg ctttgtggtc acgtctgccc ggtgggttgt
     7081 attgagctcg gggaagtgaa gtttaagaaa ggcgagaaag aacacccggt aacgttgtag
     7141 aacccgcgtc acatccggca tttggttcct gttgccggat gcggcgtgaa cgccttatcc
     7201 ggcctacaaa accacacaga tcatgatgtt atgtgcgttt atgaccgaat gcggaccaca
     7261 ttcacatcat ttcttacgcg cgtatttcag tgaatccagc gctacggcga agataataat
     7321 cgcccctttg atgatgtact gccagtatgg gtttacgccg atatacgtca ggccatagtt
     7381 gatgacggta aaaataatca ccccggtcac cacgccaatc accgtcccca caccgccgct
     7441 gaacgataca ccgcctacca cgcacgccgc gatagcatcc agctcataca taaagccgag
     7501 gttgttggtg gcagagccga tacgtccggc ttctaacatc ccgccaaagg catagaacac
     7561 gccagacaac gcgtagatca tcagcaggtt caggccgacg ttgacaccag atacttttgc
     7621 cgcttccggg ttaccgccaa tggcaaaaat gttcttaccg aagcgggttt tgttccacaa
     7681 cacccagacg aacgccaccg caatcaacgc gtagaaggtg atgtaagaga gacggaaact
     7741 ccccagcgcg acaaagccct gagcaaaggt agagaagcca ctgtcaaaac cagaaattgg
     7801 cgacgccccg acaaagtcgt aatagagcga gttgatgcca tagacgatga tcatcgtgcc
     7861 gagcgtggta atgaacggcg tcacgttgag ataagcgata atcagaccgt tgatcaaacc
     7921 gatcaccgca ccaatggcac agacaatcag aataaccagc gcaatcggca tcgtcgccat
     7981 ttccgggaac actttgttgg cgttatccat ggactgcaat aatgtcgcag ccaccactgc
     8041 cgccagccct acctgacgac cagcagaaag atcggtcccc tgggtgacaa ttaaccctgc
     8101 cacaccgagc gcgataataa tacgcaccga tgactgggtg agaatattac ttaagttcaa
     8161 cagacttaaa aatgttgggt cctggaaaat aataatcgcc agcaaaacta aaagaacgac
     8221 gtaaataccg ccctctttca ggtaagtaag aaaacttttc ttatttaacg cactcatggg
     8281 aagcccctaa tcttaaaggt gcaaagacgc aagacgcaga atttcgtttt gcgttgttgt
     8341 ttttgtatcg acaattccgg aaacgagacc attgctcatg accagaatac ggtctgttat
     8401 ccctaacaac tcaggcattt cagaggagat aataataatc cccttgcctt tcttcgccag
     8461 ttcggcaatt aactgataaa tttcaaactt cgccccgaca tcaataccgc gcgtcggttc
     8521 atcgagcatt aatatttctg gttgcgttag tagccagcga ccaataatca ccttttgctg
     8581 attaccaccg gagagcgaac caatttgcgt ccgatgaccc ggcgttttta cccgcatcga
     8641 atcaatcacc cactgggtat cgcttttcat ccgcgagtta tccagtaaac caactttatt
     8701 tttgtagttg cgaatattgg aaattaacga gttaaaacca atatccagat aggcataaat
     8761 tcccgttgag cggcgctcct cagttaccag tgcaaatccg tggtttatgg cttcgttggc
     8821 attatggtta ttgatctgtt tgccgtgcaa cgtaatggtg ccagccgatt tctcgcgaat
     8881 accaaataac gtctcaacaa tatcggtacg tttcgccccc accagcccgg caataccgag
     8941 gatctcccct ttatgcagat caaacgagac atcgcgaatc gacggctggc gcagtgacgt
     9001 taggttacgc acctcgagga tgacttcgcc cggcttgttt tctttgtcag gaaaacgctg
     9061 attaagagaa cgcccaacca tcatggcgat gatcttgtcc atcgtcagtc ctgccagcgg
     9121 ttcggtggcg atccactgac cgtcgcgcaa tacggtaact tcatcacata actggaagat
     9181 ttcttccatt ttatgcgaga tataaacaat accgcagccg cgctctttta atttacgaat
     9241 aatagtgaac agatgattga cctctttttc ggttaacgaa gaagtcggtt catccataat
     9301 cacaattttc gcgttatagg aaaacgcttt ggcgatttcg atcatctgca tttgcgaaac
     9361 ggataatgtg ccgacgcgcg cacgcggatc gatatcaata tccagttcat caaaaatcgc
     9421 tttggtttcg cggtacattt tgtcctgatc gacaaacatg cctttggtgg gatatcgccc
     9481 cagccacatg ttatccatca ccgaacgttg taataccagg tttaactcct ggtgtaccat
     9541 cgaaatacca ttttccagtg cctctttggc ggaatggaaa tcgatctctt taccctggaa
     9601 taaaatggtg ccggagtctt tttgataaat accaaacagg cattttaata atgtcgattt
     9661 tcctgcaccg ttttccccca ttaatgcatg gatagaatgt ggccggaatt ttaaattaac
     9721 gttatcaagt gccttaacac caggaaagga cttgttgata ccgctcattt ccaacaagta
     9781 ttccccggaa gacggagtcg ttgagctgac catataattt taccttgttg gccatacaat
     9841 aagggcgcag taaacgactg cgcccaatca gtcttatttc ttgctgaatt cagccaggtt
     9901 gtctttatct acgccaacat aaggtacgcg gaccactttg ttgtcgattt tccagttggt
     9961 gccatcagcc gcacctttgc catcggccag gtttttcgcc agatcaaagg tcgctttcgc
    10021 ctggttgtta gcatcgttca gtactgtgcc cgccagtgca ccggatttca ccagcgccag
    10081 cgcttctggc agcgcatcga cgccaaacac cggaatgctg gacttgttgt gtgctttcag
    10141 tgcttctacc gcgcccattg ccatcgcatc gttgttggcg ataaccactt cgattttgtt
    10201 ggcgttcggg ccagacagcc aggcgtccat cttatctttc gcctgagcgg tgtcccacat
    10261 tgcggtatct aactgtaact gttcagtttt gatgccttta tcgttcaatt ctttaatcac
    10321 gtaagtggta cgtgcttctg catccggatg gcccggttca cctttcagca gtacgaactg
    10381 aatctgaccg tctttgttca gatcccaacc ctgattcgcc gcccagtgtt tagcaatcaa
    10441 atcgccctga ataatgccgg actctttgga gtcagtgcca acgtagtagg ctttgtcgta
    10501 gctatccagc gccttacgag acggttcttt gttgaagaaa accaccggca cgttttgccc
    10561 acgcgctttc tcaatcaccg tacccgcagc tgccgggtca accaggttga ttgccagtgc
    10621 cttcacccct ttcgccagca atacgtcgat ctgatcgttc tgcttggact ggtcattctg
    10681 agaatcattc atcagcagct gaacatctgg cgcggctttc gcatcttgct caatagcctt
    10741 gcgcactaca gacataaagt tatcgtcgta cttatagatt gttacaccaa tgcgagtatc
    10801 agcagcgtgt gcagcggcac cgaataacat gctggccatc acagcagaca gggttaacac
    10861 cttcttattc atggtatctc cggtttttct tatgcagggt agtgcttgag ataaatgctc
    10921 ggcggggcag tagagttaat gaagtgttac tgaacgccga agctcacttt tttaaattcg
    10981 ttcttccatg cccggtaacg ctccagaaaa cggctttaat tgttgttatg acgctattac
    11041 ctcggtgaaa gtgattattc actgttacat acgggttaca acgttaaaac ggtgcaatca
    11101 tagctatcac attgttaaga tactgtgaaa tcactcacag attgaaagcg gttacatcgc
    11161 ctgatttgtt gagttagtga tcgccgccgc attctggcgc gttgcgacag aatggcgacg
    11221 cactaacgtc ggcataaaac agtggctggc acgaggatca atattgcctg ctgccccctg
    11281 caaggccagt tcggtggcta atttagccat tgaagcaatg ggataacgca cggtcgttaa
    11341 ttgcgggtcg gtgtaacggg cgatgggaat atcatcgaaa ccgatgattg agagatgtaa
    11401 cggaatcgca atgccattat cttttaatgc tgtcagtgcg ccagcggcca tattgtcgtt
    11461 ataagcaaat atagcggtaa gttgtagatt gcgccccagc agttcaacca tcgccgcctc
    11521 accgcccggc atgtccggcg taccagtgcc aatccagctt tccggcggaa taatatcctg
    11581 ctctttcaac gcactcatcc agcctgcttt acgcatggcg tcatcttcaa tgccgtgact
    11641 ggaagaaaga taaccaatac gttgatgacc gttattcagc aacatgcgcg tcgccattcg
    11701 ggcaccgctg agattatcca ggcaaacgca acgatgggcg taccccggca caacgcggtt
    11761 gattaacacc ataccgggaa tgttatccat aaattgcgcc agttcatcgt cactcaatgc
    11821 ttttgagtga acaatcaacg cattacaacg ctggcgaatt aacacctcaa tggcgtgacg
    11881 ctctttttcc gcttcatgat agctattgcc gattagcacg tatttctgat gctgctgagc
    11941 gactagatcc accgctttta ccagcgcgcc gaaaaacgca tcagaaacgt ccatcaccac
    12001 cacgccaatg gtgtcgctaa cctgagttgc cagcgcctgg gcattggcgt ttggccgata
    12061 atccagctca ctcacggctt tcattactgc ttcacgcgtg tcggcactga ccagcgtgct
    12121 gttattgagc acccgggaaa ccgttgccac agagacgcca gcctaacgcg ctacatcacg
    12181 aatggtgatc atattcacta cctgttatga gattgcagta aatgactgct tgctggcggc
    12241 tattttgtca gcactgaata caggacttcg tgaatcgagt cacagcaatg gaaacggtta
    12301 cagccgtttt gttaacgaat gtgatccaga tcgttatctt gatgttttag atattgccgt
    12361 tccggcagca cgcaaagtta actgacgcca gagccattcc accggcccct ggcggaagta
    12421 acgcagccag ataacagaga agaggatatt cgccagccat accggaataa caaacgccag
    12481 cagctccagg cggtcaaaat gcataaacaa accgaggtgg taaaaaagca tggtacaaat
    12541 cagcgtttgc aatagatagt tggtcagcgc catccgtccg acgcaggcga tcgcaagcac
    12601 cagcttaaag cggctcaatt gcggccagaa gccataaaac agcgacgcat agccaatcgc
    12661 ctgaaacggc gcactcagtt cccgcggcat ttgaagtaag aaggcacacc agcgatacgc
    12721 ccagtccagc tgccattgca gggcgatggc aggaaggtta atggtcaccc caatcgccac
    12781 cagcacaaaa ccagtacgac gatagtgacg taagctgaac tgccctttca gccagccgct
    12841 gcgcatcagc gccgcaccaa tgagcatcat ccctgccagt tgccagccat actgtgcgcc
    12901 cagtgccagt aaactgttgc caacaccatc ggcacgatta ctgatcgctt caacgccgcc
    12961 gtgaagcttc cagtattttt catacaaaat agccgatgca tccggcgtcc aggtgcggct
    13021 ggtctggctg tcggaaatca gccccaataa cagcaaaacg ccaagcccca ccagataaag
    13081 catgacgcct gtattaaaca ggctttttac cgatggcgca tcgcgcacca gccgccagca
    13141 gattaagccc accagcccgt aagccagcag aatatcgccg tcccagaaca ataaaccgtg
    13201 aataaaaccc agcaagacta acagcgttaa ccgcgactgg atccagcgtc tgccacgggg
    13261 cagcaacatt tgcaggcccg caccaaacag cagcgcaaaa agcgtgagga atttcacctg
    13321 gccgatgaga tcgagaaatg cccaggtcca tgcatcctgc ggcgtaatag cgccgtacca
    13381 ggcgggattg agataagccg cctttggtag cccaaaggcg ctgatgttta atagcaggat
    13441 ccccagaatg gcgacgccgc gaacaaaatc gagcgttacg ttgcgctcca tggttcctgc
    13501 cttttaatca gttgtgatga cgcacagcgc gcagaaactc gtggcgcgta ttctgactgg
    13561 atttgaacaa tccaccaaga gaggtcgttg ttgtggcact ggttgcatcg cggatgccac
    13621 gcgctttcac gcagtaatgc accgcatcaa tggagacagc cacgttattg gtgcccagca
    13681 gcgtttgtag cgcaataaga atttgctgcg tcagacgttc ctgcacctgc ggacgctggg
    13741 caaagaactg cacgatacgg ttaatttttg acagaccgat caccgaatct ttcgggatat
    13801 aagccaccgt cgctttgcca tcgatggtaa caaaatggtg ttcacaggta ctggtcagag
    13861 tgatatcgcg cacggtgacc atttcatcga ccttcatttt gttttcaatg agggtgattt
    13921 tcgggaagtt ggcgtaatcc agaccggaga aaatttcatc gacatacatt ttagcgatgc
    13981 gatgcggcgt ttccatcaaa ctgtcatcag ccaggtcgag attcagcagc tgcatgattt
    14041 cggtcatatg accagcaata aggcttttgc gcgtttcgtt atccatttca tgcacgggcg
    14101 ggcgcagcgg tgtttccagt cctcgcgcaa ctaacgcttc atgaaccagg gccgcttctt
    14161 tactgagtga tggcatttat gatttctcct gcaggtgtga cgcctccgcc ctgcgtgggg
    14221 gcaaagttat taagctgatt tacagcctga ttattgtgcg tgaggcggcg cacataatcc
    14281 agtattcaca gcgataatta ttgtaattgc cgctgacttt catcagcaga tgttaaaaca
    14341 tcgttatgca aatacggaag tgaaagttac tcacagcaca ttgaataaac ggtatgatga
    14401 agaaattgca aacaacacaa caaggagcca cgcatggaaa tgctcgaaga gcaccgctgt
    14461 tttgaaggct ggcagcaacg ctggcgacac gactccagta ccttaaactg cccgatgacg
    14521 ttcagtatct ttctcccacc ccctcgtgat cacaccccgc caccagtgct gtactggctt
    14581 tccggattaa cctgcaatga cgagaacttc accaccaagg cgggtgccca gcgcgtggcg
    14641 gcagaactgg ggattgtact ggtgatgcca gacaccagcc cgcgcggcga aaaggttgcc
    14701 aacgacgatg gctacgattt aggccagggc gcaggctttt atcttaatgc cacgcaaccg
    14761 ccgtgggcga cgcattaccg gatgtatgat tatctgcgcg atgaattacc ggcgctggtt
    14821 cagtcgcaat ttaatgtcag cgaccgctgc gccattagcg gtcactcaat gggtggtcac
    14881 ggtgcgctga ttatggcgct gaaaaatccg ggtaaataca ccagcgtttc ggcctttgcg
    14941 ccaattgtga atccgtgcag cgtcccgtgg ggaatcaaag cgtttagcac ctatttaggt
    15001 gaggacaaaa atgcatggct ggaatgggat agttgcgcgc tgatgtatgc cagtaacgcg
    15061 caggatgcga tcccgacgct tatcgatcag ggcgataatg atcagtttct tgccgaccag
    15121 ttgcaacctg cggtattggc agaagccgcg cgccagaaag cgtggccgat gacgctgcgt
    15181 attcagccgg gatatgatca cagttactac ttcatcgcct cttttataga ggatcacctg
    15241 cgcttccatg cgcagtattt actgaagtga aagtccgccc ggttcgccgg gcatcttctc
    15301 atcagaagcg ataatccact gccataaagt aacgacgtcc gtcttcgtta tagctgtagt
    15361 cgtcacgact gagatccttg tcgccaaggt tcagcacgcc tgcgcgcagt ttgacgtctt
    15421 tagtcacctg ccaggccgcg ccggtattcc agatggtata accgcccggt gttttagccg
    15481 tcgcgctgtc ggcgcgtttc tgcccggtat agtgaccaga aacatagaat gaccagtctt
    15541 ccagcgccag cggtttccag tccagcgtac cgttagcagt atggaacggc agatcggata
    15601 gcggtttgtt ttcgccgttg ctgacatcac gaccatcgtt gtaggtgtag ttgatcgaca
    15661 gtttccattc atcgttgaac ggaattttca gttcggtttc cacgccctga atacgagctt
    15721 tgttaacgtt gtagtagcta aataccggta tgcgccgtcc gttagcgccc gtctcaaaac
    15781 caacaaagtt ttggtagccc ggtgcagcgt tgacgtcaga cgtacggctg atgctgatac
    15841 gatctttcac atcgttacgg aaaacggtaa cgctggattc aaccccttcc agccagcctt
    15901 cttcacccat gtagtaaagc cccagctccc aactttcgct ggtttctggt ttcagatccg
    15961 ggctacccac aatcttacat gcgccacggc aggaattgct cgtccagtca gggctaagtt
    16021 gcaacagaga aggtgcttta aatgccgtcg cccagccccc tttcaccgtt acggtgtcgg
    16081 tggcgttata aaccaggtag gcacgcggac tccagtgttc accgtaggtt tcgtgatcgt
    16141 ccatacgcac gccggtcgtc agcgccagcg gctcgaagat ccgccattca tcttccacaa
    16201 acagcgcgta ctggctggca gacgttttgg agctggttcc cccggtcagg ttcaccgcat
    16261 cgctaagttt gtcgtgacgc cattcaccgc caaccgtgag aaactgatta atcgccgtca
    16321 gcggcaacgt gtatttgccg tcgaccgtat tgctttcgga agttatcggg ctgctgttgc
    16381 cagggttttt gttctcgact ttctcaccgt agtatttcag ttcgctggtg ccgtaatccc
    16441 aacgcccatt atggctgacg gagtagttct ggcgttccag gcggtttttg tccagcgagt
    16501 cggaatcacg atcctgacgg tcgaaaccgt atccggcagt aaaatcgtga ttttgattcg
    16561 gtgtccaggc aaattcgaca ttgccgtcgc ggctggagaa tccttcaata cgcggcgttt
    16621 ctccggtatc ggtggtcgtt gagttttgcg ggtcatcctt ttcacgtttt gccaggctgc
    16681 cgtaagcttt cattcccagc acaccatcaa ttaatggtcc actggtaaag aactgaccgt
    16741 tataggtatc accgcgatcg cgatgttcct gaatggtggt atcgacggta acggtacccg
    16801 accatttctg accgattttt ttggtgatga tattcactac accgccgagc gcatcggaac
    16861 cgtacagcga cgacatcggg ccacggacca cttcaatacg ttcgatggaa tcgaccggga
    16921 tccagttcag atcgaaatca ttgtggcgga agacggcatt gcgggagttc acgcgtttac
    16981 cgtcgacgag aatcagggta tagctgctgt ccagaccacg aatactaacg cccttacggt
    17041 tatccccttc gttcgtcagt tgtacgccag gcacttcttt gaggacatcc ttcagattct
    17101 gtaccggttt tcgctgcaga tcttcctggg taatgacgct gatactggcg ggtgcatctt
    17161 taaggttttg ttcaacggaa gatgcagtga caaccatcgt ttcgccatcg tcatcgaccg
    17221 ctaacacagg ccatacacaa gaaatagcgg acaaacacag cccgacccgt acgaaagggt
    17281 tcaacctaaa cattccatat ctccatgagg taactacgaa aataaaatgg gtatcgctca
    17341 catcttcttt acgtcccctt gcgtacggca gcatcgcggt ggacttattt gtatgcaggt
    17401 gatcatccca gaaagccgga ccagcctctg atttgtcagc cttttttgat tgtagcgtaa
    17461 cgataatgca aacgataaca gttatcaatc caaatgttaa attttatatc cgcatgattt
    17521 gtcagggaaa tggaattgaa aaagccctct cggttgagag ggcttagcaa ggaagggagg
    17581 aaacttattt cttatcgttc tgcgggaact tcatttcgct gtagcgtacg aagtgagttc
    17641 ctttaatcag cttgtagccg aaccaaataa tcaggaacag cgggatacca atatacgttg
    17701 ccgctacgcc gccccagtca atggtatctt tcaggaacgc ttcgtagttc tggcccaaag
    17761 tgataatcag acacagaatg aatgcgaaga tcggccccag tgggaagaaa cctgaacggt
    17821 acggcagatc gttaatgtcg tgtccctgca atacgtaacc gcgacggaag cgatagtggc
    17881 taatggcaat ccccagccag gcgataaaac ccgtcatccc ggaggtgttc agcagccaca
    17941 ggtataccgt ctggttgcca aacatggagg tcaggaagca caaaccggca atcaccgtcg
    18001 tcgcatacag cgcattacgc ggcacgccac cacgcgacaa tttagcgaaa atgcgcggcg
    18061 ctttaccgtc acacgccagg gtgtacagca tacgagtaga cgcatacata ccggagttac
    18121 ctgctgacag caccgccgtc agaataactg cgttcatcac cgccgccgca gagagcagac
    18181 ccgcgtgctg gaacaccaag gtgaacggac tgacgctgat gtctttaaca tcgttacgca
    18241 gcaggctcgg atcggtatac ggaataatca ggctgataat caggatcgcg aacacataga
    18301 acaacaggat tcgccagaat acctgacgta ccgcgcgtgg aatgtttttc gccggatctt
    18361 cggactcgcc tgcagcaata ccgatcagct cggttccctg gaaagagaag ccgacaatca
    18421 tagctacgcc gatcatcgcc gcaaaaccac cagcaaacgg cgcttcgccg attgtccagt
    18481 tgctccagcc cgcaggctgc gcgcctttga agataccgat aatcatcagc acgccaacga
    18541 tgataaagac aataactgtc gtgactttga tcagtgaaaa ccagtattcc gcttcaccaa
    18601 agccacgaac tgagatgtag ttcagcagga agataacgcc gaggaacaac gcactccaga
    18661 tccagcccgg tgtatccggg aaccaccagc tcatgaccag ctgtgctgca accaagtcaa
    18721 cggcaatagt caccgcccag ttgtaccagt agttccagcc cagcgcgaag ccaaagcctt
    18781 cttcaacata gttctgaccg taagtggcaa acgaaccgga aaccggcata tatgcagcca
    18841 attcaccgag actggtcatc aggaagtaaa ccatcaggcc aatcagcata tacgagagca
    18901 atgccccgcc cggacctgcc tgagaaatcg ttgcgccaga ggcaacaaaa agacctgtac
    18961 cgatggaacc gccaatggca atcatcgtca ggtgacgcgc ctttaattca cggcgtaagc
    19021 ccggcgcttc tgtagtttta gtttcggaac ccatacaaaa atgctatcca tcttaaaaat
    19081 gaggcgcgat tgtagcagac gattggcaat ccttccggca gaaatacacg gttataagac
    19141 accttcatga tcgcccaggg attataagta aagcagccaa tctcttttct ggcgaatgca
    19201 tgctgagggc gctgcgtcac gaaatcgaca cagcaccagc atgttcttgt acagcacctt
    19261 aacgcggtac attcgcggga tcgcaatagt ccagaaagcg ccgtagcgcg ttggaaaggt
    19321 gtttttgccg atgatgtata cgccacaacg tacgcatcag gcgcgggaga gggaccgcaa
    19381 cttcacttaa tgtgcctgcc tgcaattgat cttcaatcac acgtcgcgac aggcaactaa
    19441 ttcccaaccc atgacgcacc gcatgtttga ttgcctcgga gttacctaat tccatcgcca
    19501 tctcaaactt cggtaaatgt gacagcaaca gataatcgac aatctcccgc gtgccggaac
    19561 cgcgttcacg caggatccac ggcgcagcgg ccagctgttc taaggtgacc ggaccacgag
    19621 ccaacggcga agtcggcgcg gcgaaaacca ccagctcgtc ttccagccac ggttcagaaa
    19681 tgatttcagt gctgtggcac ggtccttcaa taaagccaat atcaacgcgg aaatccagca
    19741 ccgcttgcat cacgtcctgg ctgttcccaa cgctaagttc aatcggcaac tgcggataat
    19801 catggcgata acgggcgata actgcaggca gaatgtagtt accgatggta ctactggcat
    19861 agatacgaat cgcgccgttg tcttcgcgaa acagttgttc gatttcaacc gcctgttcaa
    19921 gcaatgccaa cgcacgcgga tagagcagcc gcccgtgttc attaacaacc agtcttttcc
    19981 ccacgcgatc aaacagttgc acgccaagct gcccttccag gtcggtcaag gctgcgctca
    20041 ctgctgattg cgacaacgcc agcatcaccg acgcctgggt ggttgatcca cttttcaata
    20101 cttctgcaaa aacttccaac tgccggaggg tgatgtgcat agtcgcttac cacttataaa
    20161 gattaattat aaatatataa tcaattttat ttttaaacca gttagtcgta accttatgcc
    20221 cggttaaagg agagggttat gacgaatatc accttacaga aacaacatcg tacactgtgg
    20281 cattttattc cggggttagc cctgagtgca gttatcaccg gggtcgccct gtggggtggt
    20341 tccattcccg cggttgcggg tgccgggttt agtgccctca ccctcgcaat cttgttgggg
    20401 atggttttag gcaacaccat ctatccgcac atctggaaaa gctgtgacgg tggtgtgctg
    20461 tttgccaagc aatatttatt acgtctgggt atcattcttt atggcttccg tctgacgttc
    20521 tcgcaaattg ccgatgtcgg tatcagtggg atcatcattg acgtgttgac gctgtccagt
    20581 accttcctgc tggcttgctt cctggggcag aaagtgtttg gtctggataa gcacaccagc
    20641 tggttgatcg gtgccggtag cagtatctgt ggtgctgccg cggtactggc gactgagtcg
    20701 gtagtgaaag cggaagccag taaagtaacc gtggctgttg caaccgttgt tatcttcggg
    20761 accgtcgcga ttttcctcta cccggcgata tatccgctga tgtctcaatg gtttagtccg
    20821 gaaactttcg gtatctacat tggttctact gtgcacgaag tggcgcaggt ggtggcggca
    20881 ggtcatgcca tcagcccgga tgcggaaaac gcagcagtta tttccaaaat gctgcgcgtg
    20941 atgatgctgg ctccgttcct catcctgctg gcggcgcgtg ttaaacagct gtctggggcg
    21001 aacagcggcg agaaaagcaa aatcactatt ccgtggtttg ccatcttgtt catcgtcgtt
    21061 gccatcttta actcgttcca cctgttaccg cagagcgtgg tgaacatgct ggtaacgctg
    21121 gataccttcc tgctggcaat ggcgatggcg gcgctgggtc tgaccactca cgtcagcgcg
    21181 ctgaaaaaag ctggggcgaa acctctgctg atggcactgg tactgtttgc ctggctgatt
    21241 gttggtggtg gtgcgattaa ctatgtgatt caaagcgtca tcgcataaac cactacatct
    21301 tgctcctgtt aacccgctat cattaccgtt ttcctccagc gggtttaaca ggagtcctcg
    21361 catgaaatac attggagcgc acgttagtgc tgctggcggt ctggcaaatg ccgcaattcg
    21421 cgccgccgaa atcgacgcaa ccgcgtttgc cttgttcacc aaaaaccaac gtcagtggcg
    21481 tgccgcaccg ctcacgacgc aaaccatcga tgaattcaaa gccgcctgtg aaaaatatca
    21541 ctacacatcg gcgcaaattc ttccccacga cagttatctg attaacctcg gacatccggt
    21601 cactgaagct ctggaaaaat cgcgcgatgc ctttatagat gaaatgcagc gttgcgaaca
    21661 actggggctt tctttgctca acttccaccc tggcagccat ctgatgcaga tttcagaaga
    21721 ggattgcctt gcgcgtattg ccgaatccat caacattgcg ctggataaaa ctcaaggtgt
    21781 aacagcggtg attgaaaaca ccgccggtca gggcagtaac ttagggttta aattcgaaca
    21841 tctcgcggcg attatcgacg gcgtggaaga taaatcccgc gtcggcgtct gcattgatac
    21901 ctgccatgct ttcgctgccg ggtatgattt gcgtactcca gccgaatgcg aaaaaacgtt
    21961 cgctgatttt gcccgtattg tcggctttaa gtatctgcgc gggatgcacc ttaacgatgc
    22021 gaaaagtacc tttggcagcc gcgttgaccg ccatcatagc ctcggtgaag gcaatatcgg
    22081 tcatgatgcg ttccgctgga tcatgcaaga cgaccgtttc gacggcattc cgctgatcct
    22141 tgaaaccatc aacccggata tctgggcaga agagatcgcc tggctgaaag cgcaacaaac
    22201 tgaaaaagcg gtggcctgat cgatgaacga cagggaaaag cagattctta aaattttacg
    22261 gcgtaacccg ctgattcaac agaatgaaat cgccgatatt ctgcaaatca gccgctcacg
    22321 tgttgccgct catattatgg atttgatgcg caagggtttg attaaaggca aaggatatat
    22381 ccttaccgag caggattact gtgtggttgt gggtgcaatc aatatggata ttcgcggaat
    22441 ggcggatatc cgttacccgc aagcagcctc tcatccaggt tctgttcact gttccgcagg
    22501 cggcgtcgga cgtaatatcg cgcataacct ggctctgctt ggtcgggatg tgcatttaat
    22561 atcagctatt ggcaacgatt tttacggcga gacattactg gaagaaacgc gccgcgcggg
    22621 cgtgaacgtt tcaaactgca ttcgtctgca tggacacagc actgcaactt atctcgctat
    22681 cgctaataag caggaagaaa ctatcctggc aatcaatgac acacatattc tgcaacagct
    22741 aacgcctcaa ctgctcaata cttcacggga cttgattcgc catgctggtg tggtgctggc
    22801 tgattgtaat ctcacacctg aagccctgga gtgggtcttt accattgctg atgaaattcc
    22861 gatgtttgtc gataccgttt ccgaatttaa agcgaacaaa gtaaaaaact ggtacagccg
    22921 gatccacact ctgaaaccaa cacaaaatga actggaaatt ttgtgggggc aaccgattaa
    22981 agacgacaac gatcgcatca gagctgtaaa ttcgctgcat caacaaggtg ttaaacgcat
    23041 ttttgtttat ctgaaagatg aatctgtatt ttgcagcgat aaggatggag aacaattttt
    23101 gctgaccgcg ccagcgcata cgacagtaga cagttttggt gctgacgatg gttttatggc
    23161 gggcctggta tatagctttc ttgaaggata cagtttccgc gacagcgccc gttttgcggt
    23221 agcctgcgcg gcaatttcgc gcgccagcgg cagcttaaac aaccctaccc tgtctgccga
    23281 taacgcgctt tcattagtgc caatggtgta acaatgttgc cggattcgac gcttgaccgc
    23341 atcttatccg gctcataaat tgcgcactac gctaaaccaa taaagaatcc tgcaatagta
    23401 gcactcatca ggttagaaag tgtcgccgcc gccagcgcgc gtaaaccaag ttgggcgatt
    23461 tccggcgcac ggtgtggcgc aaccgcagaa aacgccccca ccaccacccc gatagaacca
    23521 aagttagcga aaccgcacaa cgcgaaagaa ataatcgcca cggttttagc atccagagtg
    23581 ccacccgttt gcagatatgg cgagaaattg agataagcga caaattcatt gatcgccagc
    23641 ttctgcccaa tcaaactccc ggcaagattt gcatcactcc agtcaacccc catcacccac
    23701 gccaatgggg ccaacaggta acctaaaatc gactccagcg aagcatgttc aaaaccaaac
    23761 cagccgccaa caccgccgat aataccgtta atcaacgcaa ttattgcaac aaatgccata
    23821 acaactgtcg ctacaccggc ggcgattttc agcccggtca ttgcccctgt cgcagcggct
    23881 tcaataatgc tttttggcgg tgtttcggtg aaagagaggt tattaaaaga aacctgtgaa
    23941 gattccgtag ccgggcttaa caggcgggca aacaagatcc cgccagggat cgccattaac
    24001 gatgccgcca gcaaatattc cacaggtacg cccagtgcgg cgtaaccaat cattgtcgaa
    24061 ccagcaatcg aggccatgcc actacaaatc gctgtaaata attcattgcg attcagacga
    24121 tcgataaagg gtttgacgat cgccggaatt tcgttttgcc cgaggaaaat ggtggtgacc
    24181 gcgacgaatg actcgatctt gctgatattt aaagctttct ggaagatacc gccgagaatg
    24241 cgaattaaga tccccatcac accgatgtag tagagaatac tcaccagtgc agtgacgaag
    24301 ataattgccg gtaataccct gaaaccaaag ataaatcctg cgccatcaaa taaggtatcc
    24361 attttcggtc cgaccagaga accgaagata aatgcgctac ctgcgtcgct gtacgccatc
    24421 actttgtgga cgccaaaagc gactttttct gcgacccaac gccctggcgg taaccaaagc
    24481 ataatcccgc caatcacgac ctgtaacact aacgccgcgc caacggtacg caggctgatc
    24541 ttcttcttgt ttactgacag taaaaacgca attgccagca gtaccaccat tcccagaaca
    24601 cttctcatga catccatagt gattatctct tcatgtcatt ttgggcgacg gctttctgcc
    24661 catcagaaat agccgggcct gcatcttgcg atactgtccc ggcttgttat caacacttaa
    24721 tgggttttta tgtagccgcg tacgcactct tcgactaatc cccagaacca gtcggtatca
    24781 atagtgatgc cgactttagt attggcgggc ttgccaagaa cgcccagctc gtcgcagacg
    24841 gtacgcccat agcaagggcc actgttgacg tccacttcga cgtacatctc ctgggtttta
    24901 atgccatcag ggttaatcag ataaccgatg caggtggcgt cgtgcaccgg gccgccagcc
    24961 aggccgtagt tttcgaactg cgttttgaga gtgaagttca tgatgtcgct gaacatctct
    25021 ccggcagggc cgcctgccct ttccattcgt gcaattacgt ccggggtgca aacggtctgg
    25081 ttggtaagat cgaggcccat catcactaat ggaacgccgg aggtgaacac cacgcgtgcg
    25141 gcttccgggt cggcaaagat attgaactcg gcagatgggg tgaagttgcc tgtaccgtaa
    25201 gcgccgccca ttagcacaat ttcacggatt ttgggcagga ttgcgggttg catacgcatt
    25261 gccaccgcga tatttgaaag cggaccaacc ggcaccagag tgatatcgcc atcgcttgcc
    25321 ataagggtat caatgatgta tttcaccgcg tgggtacttt ctgcctggcg ggtcagcggc
    25381 tcgaataccg ggccatccag tccggtttca ccgtgaatat tatcggcaac gatttgttga
    25441 cgcataatgg gttgcggcat ccccgcataa accggaacat taatctccag tttctggcaa
    25501 acattcaggc cattaattaa tgttttatca agcgtctgat tacccgcaac aatggtgata
    25561 cctaataaat caatcgccgg atgtttcgcc gccatcatta tagcaatagc atcatcatga
    25621 cccggatcac aatccagaat aatttttctc ttttccattg tttatttcct ctgtttccag
    25681 ttgcgttatt ttttctacag caacgaaatt tcgatcgggc cgatgattga atcttaacaa
    25741 cagcgtacga atgctaaata tgagaaatct catagcggat aaacatcgtg aaagaaatcc
    25801 acaataatga tcttaagcag caattgatga gtgaatccgc gtttaaggat tgctttttaa
    25861 cggatgtttc agccgatacg cggctgtttc attttttagc gcgtgactac attgtgcagg
    25921 aagggcaaca gccgtcctgg ctgttttacc tgacgcgagg ccgcgccagg ctttacgcca
    25981 cgctagctaa tggtcgcgtg tcgctgatcg atttctttgc cgccccctgt tttattggcg
    26041 agattgagtt aatcgacaaa gaccatgaac cgcgtgcggt gcaggctatt gaagagtgtt
    26101 ggtgccttgc gctccctatg aaacattacc gcccgctgtt attaaacgac acgctatttt
    26161 tacgaaaact ctgcgtcacc ttaagtcata aaaattatcg taatattgtt tctttaactc
    26221 agaatcaatc atttccgtta gtgaatcgcc tggcagcatt tatattactc tcgcaggaag
    26281 gtgatcttta tcacgaaaag catacgcaag cggcagagta tttaggcgtt tcttatcgac
    26341 atcttttata tgttctcgcg caattcattc acgacggttt attaattaaa agcaagaaag
    26401 ggtatctaat taaaaacaga aagcagttgt caggactggc gctggagatg gacccggaga
    26461 ataaattctc cgggatgatg cagtaaaaat tattggcaat agcacgattg ccggatgcaa
    26521 cgcttaataa ggcgtacatc cggcaatgaa actcaagcta aaccaataaa gaacccggca
    26581 atggtcgcgc tcatcaggtt ggaaagcgtc gccgccgcca gcgcccgtaa accaagctgg
    26641 gcgatttccg gcgcgcgttt tggcgaaata gccgaaaacg ctccaacgac aacaccgata
    26701 gaaccaaagt ttgcaaaccc acagagggcg aacgagataa tggcaatggt cttgacatcc
    26761 agcgtgccgc tcgtttgcag atagggcgag aaattgagat aagcaacaaa ctcattaatc
    26821 gccagtttct gcccaatcaa acttccggca agatttgcat cactccagtc cacccccata
    26881 atccatgcca acggggccag cacatagccg aaaatgcttt ccagagagac attggcaaaa
    26941 ccaaaccagc cgccaactcc accaataatg ccgttgatca gcgcaatgat cgctacaaat
    27001 gccattacca ccgtcgccac accggcggcg attttcagcc cggtcattgc accgttcgcc
    27061 gcagcttcaa taatgctttt tggcggtgtt tcgctaaacg acagattttc aaacgtaact
    27121 tgcgaaggtt ccgtcgccgg actaagaata cgtgcaaaca ggataccgcc aggaatagcc
    27181 atcagcgaag cggctaacaa atagtcaatc ggcacgccca ttccggcata gccaatcatc
    27241 atcgaacccg caatagacgc catgccgcta caaatagccg taaatagctc gttgcgattc
    27301 atgcgatcaa taaaaggttt aacgatcgcc gggatctcat tttgcccgag gaaaatagtg
    27361 gtgactgcga caaaagattc gattttgctg atgttgaggg ctttctgaaa aatactgcca
    27421 agaatacgaa tcagcagccc cattacgcca atgtagtaca gcaagctgat aagcgcggta
    27481 acgaaaataa tcgccgggag tacgcgaaag gcgaagataa agcccgcacc gtcaaacaga
    27541 acatccattt tgggaccgac cagcgaacca aaaataaagg cacttcctgc gtcactgtag
    27601 gacatcactt tatggacgcc taatgccgcc tgctctactg cccattttcc cgggggaaaa
    27661 tagagcatga tgccaccaat ggcaatttgc agcagcagcg cagcgccaac cgtgcgcaag
    27721 ctgatgctct ttttatttac cgacaacaca aaggcaattg ccagcaatac caccatcccc
    27781 gcaacacttc tcattatatc cataatgatt ttcccttcat gccagtaaac ccggcgtcag
    27841 cgccaggttt tggtatgttt gatgagtacg ggcgacggct ttctgcccgc cagaaattaa
    27901 cccacgagac gctgatattc tttggcaatt tcgctcgcca gaatggcgtt attaaagacc
    27961 agttgaatat tggatttcag gctatcaccg ccggttagct ccgcaacacg cgccagcagg
    28021 aacggtgtac tctctttccc aataacaccc tgttcttcgg cttcagcaac ggcttgatca
    28081 attgctgcat tgatggtttc ttctggcatc gcaaactgtt ccgggatcgg gttcgctacc
    28141 accaggccgc cgttcaaacc ggtttgccac ttcactgcca tcgcgcgagc aatttcgcgg
    28201 gcactgtcaa gacgaatgct gacttcaaat gggctggttc ggcagaaaaa cgcaggcagc
    28261 gcttttgtct gataaccaat taacggtaca ccgaaggttt ctaaatattc tgttgttaat
    28321 cctaaatcga gaatagattt tgcccccgca caaacaacag tgacattggt atttgccagt
    28381 tcctgtaaat cggcagaaat atcgaaggta tgttccgctc cgcgatgcac accaccaata
    28441 cctccggttg caaaaacttt aatcccagcc agtgctgcaa taatcatggt tgatgcaacg
    28501 gtagtcgcgc catttttccc tgcagcaacg acaaaaggta aatcacgacg actaactttc
    28561 gtcacgttat gtccttcacg gcccagcaat tcgatttctt ctttacttaa gcccactttc
    28621 atcacaccgc caataatggc gatagtggct ggtactgcgc cctgtttacg aattgtttct
    28681 tctacttcaa ttgcggtttg ggcattttgt gggaatggca ttccgtgaga aattatggtc
    28741 gattccagcg caacaaccgg ttttttattt tttaaagcat cctgtacttc agcggatatt
    28801 tgtaataatt caggggacag agttaattca gacattctgt gttctccact aacgatctaa
    28861 cattggcaat tgataattca gggttgttgg tatattcaca ggcaagtgcc attgacgaac
    28921 atccctgcgc aaatcgaaca gaatcgataa acggcatacc gtctacccaa caggaggcca
    28981 gccccgccat catggcgtcg cctgccccag taacattgat gacattcgtt ttaatagggg
    29041 ccgaccaccc actttcaccg ttgatatcac tgtagtaaac gccatcccct cccatgctca
    29101 gcaccaagcg gttcaggccg tgttgatgaa accaggcggc gacctttgcg acatcttcac
    29161 gtccggaaag tgcaattccg ctcaacgttt cggcttcaag acggttaggt tttaacgtat
    29221 ggattttgct cagatgatcg cggattttga cgcatttcca tgccgatacc ggatcaataa
    29281 atacaggcgt ttccccgcta ttttccagaa cccagaccag cgcctcttca ctaaggttgc
    29341 aatccgcgac aataaccttt gcaccgcgaa taaactcctg atgctgggca agaaattctg
    29401 cagaaataca gtcactaata ctcatatcat ttatcgcgac aagcatttct cctgtattat
    29461 cgagcagtga taaatagctg gatgtattct cacccggaac aattaagcat ttatcgacat
    29521 aaacaccgga ttgattagtt tgcgccagta atgattgccc ataaaaatca ccacctactg
    29581 cacttaaaag ccaggcattt ttccccagta acgccagatt atgagcgata ttccttccca
    29641 cgccacctgg ggtaaattta atttttccgg gattcgaatc cgcataattc aatggatgat
    29701 gtgaataccc agccacatca atattcgccg aaccaataat tactatgtag tccttttcgc
    29761 gcatagcacg ccccctggca ataaattgcc aacgaagaaa ttgggtaaaa tagccattac
    29821 agtcaataag actattagag cacgtgttca gatttaaaca catgttcata ataagcatat
    29881 ttaacgtaat gtaaatcgat cagaaaaaac gaaatcggcc agatatgaag gcggtcacaa
    29941 agtttgtcta tttatgcagc aacaaaagag aaagttgtga tgtgtaacac acaatatggc
    30001 aggcaagaaa aagggcagag atgtttctgc cctccagatt gttgacaacg cgcgctttgt
    30061 tcatcctgga tgtggcgtaa acgccttatc cgacctacaa aagcgcaaaa aattcaatat
    30121 attgcagggt tcatgtaggt ctgataagca tagcgcatca ggcaattttg cgtttgtcat
    30181 catcggggca gagatgtctc atccccgata atgaagataa attatgcgtt tttttctact
    30241 atttgtgttt ccgggcgttt caggaaggca taggccaaac ccgccaccag cgtaccggca
    30301 ataattgcta ccaggtaacc caataccggc gtaatagcgc cagggatcag cagaacaaac
    30361 agaccaccgt gtggtgccat cagtttcgca ccaatcgcca ttgagattgc gccagtcagc
    30421 gccccaccaa cgatacagca cggtagcaca cgcatcggat cacgagcagc aaacggaatt
    30481 gcaccttccg aaataaagca cagaccaagt accagagcgg ctttgccacc ttcctgctgc
    30541 gctttgtcga atttgcgacg cgccaccatt gttgccagac ccattgccag cggtggcacc
    30601 atacctgccg ccataatcgc cgccatcggg ccataggttt gagtactcag cagacccaca
    30661 ccgaatgcgt acgctgcttt gtttaccgga ccgcccatgt cagtacacat catgccaccg
    30721 aggatcgccc ccagcagaac cgcattcgca gtccccatgg tctgcagcca gtgagtcaac
    30781 ccatcgagaa tgccagcaac cggtttaccg attaggtaga tcatcgccag accgaccacc
    30841 agactggaaa ttagcggaat gatcaggatc ggtttcagcg cctccatact ctgtggcagt
    30901 ttcaattgcg tactgattaa cttcgcaatg taaccagcca ggaagccagc aattataccg
    30961 ccaataaagc cagaaccggt gctgaccgcc agcataccgc caatcagacc cggtgtgagg
    31021 cccggacgat cggcaatgga gaaggcaata taacctgcca gtaccggcac catcagcgca
    31081 aaggctgaac caccaccaat ctgcatcagc gccgctgcca acgtacccgg ctctttaaac
    31141 gcttcgatac caaaagcaaa agaaagcgcg atacacagac cacctgcaac aaccatcggc
    31201 agcatgtaag agacgcccgt cagcaagtga cggtaagcgc ctgcactctc tttcttacct
    31261 tcagtggtcg ctgtttgagc tttgcccgct ggttcatacg gcgttgcttc agcaaccgct
    31321 ttatccagtt cctgcgcagt tttcttcagc gccagaccgg tagaggtgcg atacatcggt
    31381 ttaccagcaa atttcgccag atccacttcg atatctgccg ccacaatcac cagatccgct
    31441 gctgcgactt cttcaggggt gattgcattg cccgcgccaa cagaaccacg ggtttcaact
    31501 ttcacccacc agccacgttt tttcgcttcg gtttcaatgg cttcagccgc cataaaggtg
    31561 tgtgctacgc cagtcgggca agcagtcacc gcaactacgc gtttcggacc gctggcagca
    31621 accggtgctg tcgcagcgac cggcgcagtg taaggtttcg catggccttt ggcttcactc
    31681 aggaacagct cagggtgcgc aactgcccgg gaaatatcac ccagccagac atttttaccg
    31741 ttcagcgcgc tgtcattcgg gatggaatca ccgagaacaa tcgccatttc tgcgtcgttc
    31801 ggattgtcga tgatttccag ttttgctttt cgcgccgccg cgcccagcag ggtcttcgcc
    31861 atataggcgc gtgcctgacc gagattagcg tcaataatca gcagcgtttt cattatgcct
    31921 ctcctgctgt cagttaaaag gttgtaagtc gacgcgcgcc atcattgcgg ccaactgcgg
    31981 acgatcggta atacccacat tgctttgact taccgccagg gctgcaacag ctgtcgccag
    32041 acgcagtgtg tgttcactgg attcacgcat cagcaagcca taaatcaggc caccaaccat
    32101 agaatcccct gcgccaacgg tgcttacgac atcgactgac ggtggtttgg cgatccattc
    32161 gccggaggca ttaacccaaa gcgcgccttc ggcacccagt gaaataacaa catgcgcaat
    32221 accttgttca cgtagcgcat gtgcagcttc aatcacatct ttcatttcag gcagtttacg
    32281 gcctgcccag atttccaact cgcggcggtt aggtttcacc agccacggtg ccgctttcaa
    32341 acctgctact aacgcttcac ggctactatc aaagataatg caaggacact gactacgcag
    32401 gcgagtcatc cagtcggtga acgcttccgg gctgacgcct gacggtaagc ttccgctgac
    32461 acagaccata tcgaactgac cgagccagct cagagagtca gtcacaaagc gttcccagtc
    32521 ggcgagggtg acttcaaaac ccgagaagtt gaagtcggtc acttcgccgt ctttttccgt
    32581 cagcttaacg ttaattcggg tgcgcccctg tacaacctgg aaacggttgg caatgcccag
    32641 ctcgctgaac agttgctgaa aaccatcctg attgtcttta cccaggaagc cgccaacggt
    32701 gacatcaatt cccaggtctt ttaatacttt ggccacgttg atgcctttac ccgccgcatg
    32761 cagaccggtg gttttcacca ggttcacttc gccgcgttca atttccgggc agaaaccaac
    32821 aaggtcataa gccggattaa gggtgatcgt agcaacacgt ctgctcatta tgcgccctcc
    32881 ccaagaccag cagcgatagc gtcgccaatg gctttcagcg cctgttcagc atctgcaccc
    32941 tgggcggtaa agcgtaggcg atgacctttc ttaacgccaa gtgccacaac tttcatcaga
    33001 ctacgtccgt ttgccggttt gccggtgcca tcaaggtttg tcacggtaat atcactgtta
    33061 aattgtttaa tggtattgac cagcatggta cctggacgag catgcaggcc gtgttcattg
    33121 cgcaccacaa actccgcgct taacacgtcg tcggtcggcg catcatcgct ggtcagcagc
    33181 gccagcaacg ttgccgcatc cgctttcagc aagcggtcag ctttattgtc gagcaataaa
    33241 tcagcgagac gcttaagaac cgcgatgggc tgatcgtcat tcatcgccac actcaccagc
    33301 atggctgccg tttcgccgtc cacatcaaaa gcatttgccg cacggcttac cgcaatcgcg
    33361 ctacgcagat tgccttcggc gctatcgctc agccagatac cctgtccgag attcagcggt
    33421 tgttcattga tggctttggt gacgaaagtg gcgtcaactg cccccacctc tttcagacgc
    33481 gcagcgttca gcgcctgaag agtcagcaga tcgctggcga cgatatccag cgtcagcatt
    33541 tcgttgtcga gcttcagctg ctcactctgc ttttcgccca tcagtaatgc gcgaagttct
    33601 tctgctgttg ttgctgactt cagttgttca gcaacggaat catcgctcag tacgtgggtc
    33661 agctggcgta gcaggcccag atgttcatcc gagctggcag caataccgat tgccacgtac
    33721 gctacctgac cgtcacccca ggtgacgcct tccgggaact gaaatacctg aacgccggtt
    33781 ttcagcacct gatcgcgggt gtcggtagtg ccgtgtggaa tagcaatacc attgccgagg
    33841 aacgttgagg tttgctgttc gcgcgccagc atgccattga cgtagccttc tgctacatta
    33901 ccggcctgca ccagcgccgc agcgacctgg cgaatcgcct cttctttgtc tccggccttt
    33961 tcgcccggat ggatgtcctg tacggataac tggaacatag ttctcctctc ttgctgaatt
    34021 gaaacgattc agcctctatg agaaaaaaag cgccaacctg gcttagggtt aaagacaaga
    34081 tcgcgctgaa acgtttcaag aaagcataat acttctgttt cagcacgcaa ggaaagccgt
    34141 aaagttatga gcaaaagttt gatgtgctgc acaatttttt gtcatttttc ctaattgctg
    34201 atgggaaaat ccgttatcag caatttcatt tcagcagcgt cagcagccaa tcagcaacag
    34261 tgaaatggca ttttgattat tcgtggcgct tttgatttcg ctgtctgttt attttctgac
    34321 aagcagcgta aactccgcgt cttcctcttc cagtgatcga cctgcatgca taactccccc
    34381 gcagtctcca gcgcgaaatc gtttgacctg acctcgacgg cgtttttaat cgttgccttt
    34441 ctcaccggta ttgcgggtgc tctgcaaacc ccgacactca gtattttcct taccgatgaa
    34501 gtacatgccc gtccggcgat ggtgggattc ttctttaccg gcagcgctgt cattgggatt
    34561 ctggtaagtc agtttctcgc tgggcgctct gataagcgcg gcgatcgcaa atcgctgatt
    34621 gtcttttgct gcctgttagg cgtgctggcc tgcacccttt ttgcctggaa tcgcaactac
    34681 tttgttttgc tattcgttgg cgtctttctt agcagctttg gctcgaccgc taacccgcaa
    34741 atgtttgccc ttgcccgtga acatgccgac aaaaccggac gtgaggcggt gatgttcagc
    34801 tcttttttac gcgctcaggt ttcactggca tgggtcattg gcccaccgct ggcttatgcg
    34861 ttagcgatgg gtttcagctt tacggtaatg tatctgagcg cagcggtagc gtttattgtt
    34921 tgcggcgtga tggtgtggct gtttttaccg tcgatgcaaa aagagcttcc gctggcgacc
    34981 ggcacggttg aagcgccgcg ccgtaaccgt cgcgatacgc tactgctgtt tgtcatttgt
    35041 acattgatgt ggggctcgaa cagcctgtac atcatcaaca tgccgctatt tattatcaac
    35101 gaactgcatc ttcccgagaa actggccgat gtgatgatgg ggaccgccgc cgggctggaa
    35161 atcccgacga tgttgattgc cggatatttc gccaaacgtc tgggtaagcg tttcttaatg
    35221 cgcgttgctg ccgtgggtgg cgtctgtttt tacgcaggaa tgctgatggc gcattcacct
    35281 gtcattctgt tgggcttgca gctgctaaat gctattttta ttggcattct gggcggcatc
    35341 gggatgctct attttcagga tctgatgccc ggtcaggcgg gttcagccac cacgctctat
    35401 accaacactt cgcgcgtggg ctggatcatc gcaggatcag tggcgggcat cgtcgccgaa
    35461 atctggaatt atcacgctgt gttctggttt gcgatggtga tgattatcgc cactctgttt
    35521 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
    35581 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
    35641 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
    35701 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
    35761 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
    35821 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
    35881 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
    35941 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
    36001 ggcaaactgc tgctggttaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
    36061 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
    36121 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
    36181 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
    36241 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
    36301 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
    36361 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
    36421 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
    36481 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
    36541 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
    36601 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
    36661 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
    36721 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
    36781 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
    36841 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
    36901 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
    36961 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
    37021 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
    37081 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
    37141 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
    37201 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
    37261 cgccgtgagc gcggccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
    37321 ggccatgtgg tgaaaaacgc ggtgctggga atggcagaaa aatgttcgcc agaactcgcc
    37381 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
    37441 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
    37501 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
    37561 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
    37621 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
    37681 gtccatatca gtgattgcat gcaggatcgc gtatttcgcc atgctgccag aacattaatg
    37741 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
    37801 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
    37861 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
    37921 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
    37981 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
    38041 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
    38101 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
    38161 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
    38221 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
    38281 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
    38341 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
    38401 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
    38461 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
    38521 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
    38581 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
    38641 ctgttaaccg caccagtcta tgaactgtgg atagatctgc gcgccacctt gtgcattctc
    38701 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
    38761 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
    38821 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
    38881 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
    38941 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
    39001 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
    39061 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
    39121 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
    39181 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
    39241 ctgatttcca gcagcgaagc tgactggaat gctttgcaga gcgcgctgtt gaagcttcgt
    39301 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
    39361 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg cctcgcgctg tttctttcct
    39421 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
    39481 ttaatcagaa actggtcgaa agtaaggcct ttttgtggct ggttgcattg accaacaatc
    39541 gcgctttcga cggttgttct ctgctggcga tgggaatgtt gatgctgagt ttctggctga
    39601 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
    39661 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
    39721 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
    39781 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
    39841 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttgtg gtttttgcat
    39901 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
    39961 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
    40021 tttttgacaa atcactaccg ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
    40081 ttaacatcat caggcttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
    40141 catccatcgc gcgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
    40201 aatcgtactt taattgacca atcccgctta ttaactttct gtatcacttt ttcttataaa
    40261 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
    40321 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
    40381 taaggaattg tttcaacatg cccaggtaat tagtctcgtg ccgcttggca tttttttata
    40441 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
    40501 ttttgagata tatcttgcgc gggatccccg cgattgcagt agcggttctg ctttctgcat
    40561 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
    40621 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
    40681 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
    40741 gcactaaaaa aggtatcgat tgttctggtt ttgtacaacg tacattccgt gagcaatttg
    40801 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
    40861 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
    40921 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
    40981 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
    41041 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
    41101 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
    41161 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
    41221 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
    41281 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
    41341 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
    41401 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
    41461 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
    41521 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
    41581 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
    41641 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
    41701 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
    41761 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
    41821 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
    41881 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
    41941 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctctgc tattacctga
    42001 tgagcgtacg tatgcgcccc ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt
    42061 acgtggcgta tcaaccggtg gtggatacac aagctttgcg agtaacgggc ctggaagtac
    42121 tgctacgctg gcggcatcct gtcgcgggag aaattccccc ggatgccttc attaactttg
    42181 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
    42241 atgccgcaga attagaaaaa gtgctgccag taggcgtcaa atttggtatt aacattgcgc
    42301 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
    42361 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
    42421 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
    42481 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
    42541 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
    42601 cggtgctgac gctggcgaaa cgcctcaata tgctgacggt tgctgaaggg gtcgaaacgc
    42661 cggaacaggc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
    42721 gtcgcccttt accgctggac gattttgtcc gctggctgaa gaaaccgtat acgccgcagt
    42781 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
    42841 gatgagtaaa aggaaatcca ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
    42901 tgttcacctt tggtgtgcag gcgcaggcta tcaaggaaag ctatgccttt gccgtgctgg
    42961 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
    43021 aaggtgggca gataacgttg tcagccctcg gcaccttcga taatttcaac cgctatgcac
    43081 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgctattt acgacttccg
    43141 atgacgaacc aggcagttat tacccgctga ttgctgaaag cgcacgctat gctgacgatt
    43201 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tcgcccatta
    43261 ctgcccgcga tgtagagttt acttttcaaa aatttatgac cgaaggcgtg ccgcaatttc
    43321 gtctggtcta caaaggcacc accgtcaaag ccattgcacc gttaaccgtg cgcattgagt
    43381 tagctaaacc cagcaaagaa gatatgctga gtctgttttc gctgccgata tttccagaaa
    43441 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
    43501 cgtaccgcgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
    43561 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
    43621 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
    43681 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
    43741 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
    43801 ttaatatcca acgtccggta ttcagcgatc gccgggtccg ggaagcaatc actctcgcct
    43861 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
    43921 attttcagaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
    43981 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
    44041 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
    44101 aaacgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
    44161 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
    44221 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
    44281 accgcatgcg cagtcgcgac tatgacatga tgccgcgcct atggcgggcg atgccgtggc
    44341 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
    44401 ccggcgtgca aagcccggtt atcgactcgc tgatcaacca aattattgcc gcgcagggaa
    44461 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
    44521 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
    44581 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
    44641 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
    44701 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
    44761 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
    44821 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
    44881 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagaccca gaagtgatcg
    44941 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
    45001 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
    45061 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
    45121 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
    45181 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
    45241 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
    45301 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
    45361 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
    45421 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
    45481 gggtaagtga gaaaaacatt ctctggaaac atgtgttccg caacgccatg ctgctggtga
    45541 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    45601 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    45661 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    45721 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    45781 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    45841 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    45901 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    45961 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    46021 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    46081 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    46141 gctgggaacg gatgccaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    46201 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    46261 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg ggacaacgct ttattgaagt
    46321 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    46381 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    46441 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    46501 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    46561 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    46621 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    46681 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    46741 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    46801 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    46861 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    46921 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    46981 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    47041 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    47101 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    47161 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    47221 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    47281 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    47341 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    47401 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    47461 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    47521 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    47581 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    47641 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    47701 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    47761 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    47821 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    47881 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    47941 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    48001 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    48061 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    48121 taattcttaa gccctcactg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    48181 tacaggcgca aatattgacg ctattgaaat cattgcaaca aaagcatcaa ctggcctatt
    48241 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    48301 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccgca ccgcagcagg
    48361 agtatacgcg tcagctactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    48421 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    48481 cgatttacaa acaggcacgg ctcaccttca cactccagca ccgaacatgg aacttcgatg
    48541 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    48601 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    48661 accttcgaac ctgcgaaacc agcagaccag cgaaagttct gcggtagacg atggacgatt
    48721 gagagttgta atgaagtcac ttattttccc cggaagcaca tttacttcac aatttattta
    48781 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    48841 cgcctgcatg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    48901 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    48961 tttttacata aattgatttt acataaaata aacatatatc gggggaatga tagatttgtg
    49021 gttgacatgc atcaacaaag gaagcctttt agcttcctcg ttgtgcaata gatcaccgtt
    49081 ttttcggtcg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    49141 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    49201 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    49261 actcatcaag aatgaccgcc atcgcattgg atgacaccat agacacgcag cccacaaacg
    49321 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cagcgcactg atgaccatcc
    49381 acgctgccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    49441 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    49501 agtaataacc aaagttttcc ggtgcaacgt ggttgatttc aatgtagaca aacggtccgg
    49561 cgctcaggaa cgagaacatt ccggcaaagc tgaaaccgct ggccagcata taactcagga
    49621 cacgcttatg acggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaaaggct
    49681 ggcgaggatc cagaagatgt aatgccagct cagccacacc agcacccagc cgccaactat
    49741 cggtgccatc agcggtgcaa tggtcgtcac cagcatgaca aacgacatca tccgcgagaa
    49801 ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg ccgcagccgc
    49861 agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg ccaacgcaca
    49921 cgccaccgcg gcggcggcaa acaccagcgt accgccaaga accaccggct tacgcccgaa
    49981 gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa tataagtact
    50041 cagggtcatc tgcgtactgc ccgccggtac gccaaactgc gctgaaatta ccggtagcgc
    50101 gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa ggataaaaac
    50161 aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt cattgaaaag
    50221 ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg gggctaaatc
    50281 agcatccagc gtaatgccgc caatacgttc acgatgcagc tcaaccacat ggttacccac
    50341 ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca gacgaacctg
    50401 cgttggggta atcacttcca gcaccgccgg cttagtgaga tctttttcgt tatgcagctg
    50461 cacgccttta gcaaattgtt ctgccgtatc gtcagctacg ggtgatacca gcgtcaccag
    50521 ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact gaccatcatc
    50581 tgtcattagt accagaccgg tggtatcgat atccagccgc cctgcggcgt gcagtttcca
    50641 cgctaccggt tcatctagaa aatagagcac cgttgggtga tcagggtcgt ccgtggagca
    50701 aacataaccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct gcgccagcgg
    50761 gttgccatcg taagcgacat catgttcagg aagcagtttg aacgctgcat tacggacgat
    50821 ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag cacggctaac
    50881 gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc ctgtaaaaaa
    50941 cggaggtcgg gtaatgaccc gaatatctga actgttgtct gcccagtata gcggtctaat
    51001 aacatccctc aagggaaaaa gatccgtggc atactattag cagaataatc tacctacgcg
    51061 agaccatgat ttttacactt cgcccatatc agcaagaagc cgtggatgcc acgctcaacc
    51121 attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggtgca ggtaaaagcc
    51181 tggtgatagc ggaactggca cggctggctc gtggtcgcgt actggtgctg gcacacgtta
    51241 aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa gccgatattt
    51301 ttgccgccgg gctgaagcgt aaagagagcc acggtaaagt ggtatttggc agtgtgcagt
    51361 cggtcgcccg taatattgat gcctttcagg gtgaattttc gctgttgatt gtcgatgaat
    51421 gtcaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact cacctgacaa
    51481 aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga ttgggcaaag
    51541 gttggatcta ccagttccat tatcacggca tggtacgcag cgatgagaaa gcccttttcc
    51601 gtgactgcat ttatgagctg cccctgcgtt atatgattaa acacagctat ctgacgccgc
    51661 cagaacgact ggatatgcca gtagtgcaat atgatttcag ccgcttgcag gcacagagca
    51721 acgggctgtt cagcgaagcc gatctcaacc gtgagctgaa aaaacagcaa cgtattaccc
    51781 cgcacatcat cagccagatt atggagttcg ccgaaaagcg taaaggagtg atgattttcg
    51841 ccgcgacggt cgaacacgca aaagagattg tgggattact gcctgccgaa gatgcagcac
    51901 tgattactgg cgacaccccc ggcgctgagc gcgatgtgtt aattgaaaat tttaaagccc
    51961 agcgttttcg ctatctggtc aacgtcgcgg tactgaccac cggatttgac gccccgcacg
    52021 tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa caaattgtcg
    52081 ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat tatgcgggta
    52141 atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt gacaacgttc
    52201 cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg aaaacgaccg
    52261 ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt gaagatgacg
    52321 acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg caatgtaacg
    52381 cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg gttgatccgg
    52441 atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc tgtagcggca
    52501 tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc tattacgatg
    52561 aagacggtgc ggatgtgagt gagcgtttcc gtctgcaaac acctgcccag cgtaccgcct
    52621 tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catcccgctg cgctggatca
    52681 ccgccgccga tatcctcgcc cagcaagcct tattgcgacc cccggatttt gtcgtcgccc
    52741 gcatgaaagg ccagtactgg caggtgcgtg aaaaagtgtt cgattacgaa ggtcgttttc
    52801 gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag gcgattgagt
    52861 atagaatctc acccgctttt gcatacgcaa agcagatcac ttacctgttg ctgggtcgcc
    52921 tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga agtacgtaaa
    52981 gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc ggcaatcatc
    53041 tacggtggca aagaagcgcc gctggctatc gagctggatc acgacaaagt catgaacatg
    53101 caagctaaag ctgaattcta cagcgaagtt ctgaccatcg ttgttgacgg taaagaaatc
    53161 aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca gcacatcgac
    53221 ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg gggttttttg
    53281 tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga taaggcgttc
    53341 acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg tccggcgctg
    53401 caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc agggtatcgg tcgccggctc
    53461 ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc caccgccgct
    53521 accggcaaac ttcgtcaact gacgcagtgt gctccggtct gccgggaagc tctcttccag
    53581 ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc ctgccagctc
    53641 tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat tacagtagct
    53701 ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt cggcagtgaa
    53761 atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gtgttcaatc cttcgctggc
    53821 cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt tcaggaaagt
    53881 gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac gcgcaacaat
    53941 atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt cgttaacacg
    54001 catactgctc aggttgctca gaactgccac cagcagatac tccaccgcca gataacggta
    54061 gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt cgtcacgcag
    54121 gcgaccagtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac gctgtaagcg
    54181 cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat ttttggcgct
    54241 atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt ccagcaatga
    54301 atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt gcagagcaat
    54361 ctggttgata tccagaccca tgataaactc tcctttaaga ccgggcggta ttcaaccacc
    54421 gccggttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc gtaacgagaa
    54481 tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg ttgccctttc
    54541 atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtgatg aacaggttga
    54601 acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga ccgacctttc
    54661 cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg ccccggccca
    54721 acgccaggca attgccaact cttttgcccg cgccttacag tcctctatca acgaagacaa
    54781 agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc taccgtgaaa
    54841 aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt ctgctttcgc
    54901 tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt gctggtcgca
    54961 tttattccta cgtaagcatt atcggccatt tcagcttcct ggtgttcgcc acctacttgc
    55021 tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg tttttgtccg
    55081 tcattctggc aacggcggga atgacgctat tactgatcga tagcgaagtc tttactcgtt
    55141 tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac gaaaatgaga
    55201 tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg cttgaactgg
    55261 tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga cgcttcgcgc
    55321 gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg tatatctggg
    55381 ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg ctttcgtacc
    55441 cgatgacggc gcgacgtttt cttgagaagc atggtctgct tgatgcgcag gagtatcaac
    55501 gccgtcttat tgagcaaggt aatccagacg ccgtttccgt tcagtatccg ttaagcgaac
    55561 tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc gatggcctga
    55621 actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag caaaatattt
    55681 cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt ggcctgttct
    55741 atggtatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct gcggcattaa
    55801 ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat ggctttacca
    55861 gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta cgcacccaat
    55921 ccgacgagca gaccgccacg cagtggatca actggctggg ccactacgca caagaagata
    55981 accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc aatcagcagg
    56041 catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc aaccgcgtgc
    56101 tcaatgcact gcgtgattct ggcaaactgg acaatacggt tgtgattatc actgccggtc
    56161 ggggtattcc gctgagcgaa gacgaagaaa cctttgactg gtcccacggt catctgcagg
    56221 tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgcg ctgactgatc
    56281 ataccgatct gatgacgacg ctgatgcaac gcctgctaca tgtcagcaca cctgccagcg
    56341 aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg gttactgcag
    56401 cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg aacaataacg
    56461 gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa ccacagttaa
    56521 gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga ttaattataa
    56581 atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat tagcagccac
    56641 gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg gtcggaggtt
    56701 caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacctttacg gttggttttt
    56761 ttatatctgc aattaatttg ataaacagac cgtgacacat cacagcctgt ttattttctg
    56821 ttatcagaac gtccagacca ctcccgcctg agtattccac ggcgattcta cccctgcgcc
    56881 attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg accttatccc
    56941 agcctgataa acgccacggg ttccagccac atcattatta aatttgccat cgtcattaac
    57001 tttcacatgg ttagaatcgg cctattcctg acgcacggcg gctttcagcc agggttccaa
    57061 cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt cagcgcgtaa
    57121 tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt cctgaccatc
    57181 tgtggtaaag ccggtaaagg cgagataggg gctaacactc cacaatccgt caacccaacg
    57241 gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa acgctgttgc
    57301 cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca ccaccccatc
    57361 aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtgt agctatcaat
    57421 attgcctttg ccgccgcgat caaaaccaat atcagaatga gagtaatcaa agaacaagcc
    57481 gcgaattgtg ctgctttctt cacgggagaa acggctatcg ataccgagcg tcaggcccgt
    57541 caatgtttgc tcaaagccag ctcccgcatc agtggtcacg ttgttgcggg tgttaattgc
    57601 cgaactccac atcgccgtat cgtaattaac gccttttacg ctaccaagac gctcacgcac
    57661 ggtgtccagt tctgcatcaa ataccagcgg ttgtgctgct gccatgttca gcacatcagt
    57721 ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt tgccattatc
    57781 cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc ccaacgtgaa
    57841 tgcagcatca ccgccgcccg tcgttaccag tgtaaggcta tctcctgctg ccgggctggc
    57901 ccccgtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccgatga cgttgagctg
    57961 atcgctctga tgattagcca tatcggtacg catataaaat tgccgtttcc cgacagggta
    58021 ttggtggtca acgtaatgaa attttccgca cttgccgtca cagatgaacg agtcactggc
    58081 tcagccatca taatcacatt tgcgccggac atcgcaaggt tggctaagtt gaggcgagtg
    58141 ttttggttcg tcgtagtgat atcggcaatg atggtccatg cgctattttc gagcgaaacg
    58201 tccgcttggc tggtatccac ggcaccatgc gttctaaaag aagcgccctc agaaaccact
    58261 accttaccca ccaacatagc gggtgcataa tttattttcg gcttcatgac ctcaagaatg
    58321 ccatcattcc ctctgactga cacgttaacc attgtgccag cccagacatt agcgcggcca
    58381 ttgttgataa ccatattttc agagatggct ttagaggaat acgtgatact tccattctga
    58441 tctgatcgac cgagatcata tacagcattt gcctgcactt tcgtaccagt atcctttccc
    58501 agcgattgca tagtggcatg ctcatcaagg atagtgtcaa tggcctgagt gtcctccata
    58561 acaatgagcc cggagccatc atccagttca taattttttg acacaccatc ctttatacta
    58621 aattggcctt tactgtttgt accactcact tccagcgcat tcgttgagac aattaatttc
    58681 ccgcctgctt ttttatcaac gccagttgca gtcccgccat ccataacctc cagtaagccg
    58741 ccactatcca cagtggtatt ataagcgaag tcattttctt caactcgcaa acttccgccg
    58801 ttttccaaca gcatattgtt agcaatacca ttcttgattt cgaactgacc gagacggttt
    58861 gttccgaata cctccatgac ccgcgtggtt gttttaatag caccgcctgc tttctgatct
    58921 accgcaaatg ctaatccccc gtctttaacc tccagtaatc ctcccttttc gatagcggca
    58981 aagacggcaa tcccatcttt ttcaacaatt aagcgcccgc ctttaatcgt gggatcattc
    59041 gccgcg
//
LOCUS       DAZPLI010000008        58366 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZPLI010000008 DAZPLI010000000
VERSION     DAZPLI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58366
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.008"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..161
                     /locus_tag="ACHUU3_000441"
     CDS             <1..161
                     /locus_tag="ACHUU3_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR2080614.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(199..1260)
                     /locus_tag="ACHUU3_000442"
     CDS             complement(199..1260)
                     /locus_tag="ACHUU3_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR2080615.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1473..2936
                     /gene="bepA"
                     /locus_tag="ACHUU3_000443"
     CDS             1473..2936
                     /gene="bepA"
                     /locus_tag="ACHUU3_000443"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR2080616.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2957..3316
                     /gene="arsC"
                     /locus_tag="ACHUU3_000444"
     CDS             2957..3316
                     /gene="arsC"
                     /locus_tag="ACHUU3_000444"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR2080617.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3454..4155)
                     /locus_tag="ACHUU3_000445"
     CDS             complement(3454..4155)
                     /locus_tag="ACHUU3_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR2080618.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="ACHUU3_000446"
     CDS             complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="ACHUU3_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR2080619.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5625..6251)
                     /gene="upp"
                     /locus_tag="ACHUU3_000447"
     CDS             complement(5625..6251)
                     /gene="upp"
                     /locus_tag="ACHUU3_000447"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR2080620.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6576..7613
                     /gene="purM"
                     /locus_tag="ACHUU3_000448"
     CDS             6576..7613
                     /gene="purM"
                     /locus_tag="ACHUU3_000448"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR2080621.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7613..8251
                     /gene="purN"
                     /locus_tag="ACHUU3_000449"
     CDS             7613..8251
                     /gene="purN"
                     /locus_tag="ACHUU3_000449"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR2080622.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8402..10468
                     /gene="ppk1"
                     /locus_tag="ACHUU3_000450"
     CDS             8402..10468
                     /gene="ppk1"
                     /locus_tag="ACHUU3_000450"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR2080623.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10473..12014
                     /gene="ppx"
                     /locus_tag="ACHUU3_000451"
     CDS             10473..12014
                     /gene="ppx"
                     /locus_tag="ACHUU3_000451"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR2080624.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12053..14296)
                     /gene="pdeF"
                     /locus_tag="ACHUU3_000452"
     CDS             complement(12053..14296)
                     /gene="pdeF"
                     /locus_tag="ACHUU3_000452"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR2080625.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14478..14630)
                     /locus_tag="ACHUU3_000453"
     CDS             complement(14478..14630)
                     /locus_tag="ACHUU3_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2080626.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14648..14839
                     /locus_tag="ACHUU3_000454"
     CDS             14648..14839
                     /locus_tag="ACHUU3_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR2080627.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            15150..15668
                     /locus_tag="ACHUU3_000455"
     CDS             15150..15668
                     /locus_tag="ACHUU3_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR2080628.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            15684..16223
                     /locus_tag="ACHUU3_000456"
     CDS             15684..16223
                     /locus_tag="ACHUU3_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR2080629.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16318..17895)
                     /gene="guaA"
                     /locus_tag="ACHUU3_000457"
     CDS             complement(16318..17895)
                     /gene="guaA"
                     /locus_tag="ACHUU3_000457"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR2080630.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(17964..19430)
                     /gene="guaB"
                     /locus_tag="ACHUU3_000458"
     CDS             complement(17964..19430)
                     /gene="guaB"
                     /locus_tag="ACHUU3_000458"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR2080631.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            19592..20962
                     /gene="xseA"
                     /locus_tag="ACHUU3_000459"
     CDS             19592..20962
                     /gene="xseA"
                     /locus_tag="ACHUU3_000459"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR2080632.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(20959..21174)
                     /locus_tag="ACHUU3_000460"
     CDS             complement(20959..21174)
                     /locus_tag="ACHUU3_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR2080633.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(21243..22715)
                     /gene="der"
                     /locus_tag="ACHUU3_000461"
     CDS             complement(21243..22715)
                     /gene="der"
                     /locus_tag="ACHUU3_000461"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR2080634.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(22833..24011)
                     /gene="bamB"
                     /locus_tag="ACHUU3_000462"
     CDS             complement(22833..24011)
                     /gene="bamB"
                     /locus_tag="ACHUU3_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR2080635.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(24022..24642)
                     /gene="yfgM"
                     /locus_tag="ACHUU3_000463"
     CDS             complement(24022..24642)
                     /gene="yfgM"
                     /locus_tag="ACHUU3_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR2080636.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(24660..25934)
                     /gene="hisS"
                     /locus_tag="ACHUU3_000464"
     CDS             complement(24660..25934)
                     /gene="hisS"
                     /locus_tag="ACHUU3_000464"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR2080637.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(26045..27163)
                     /gene="ispG"
                     /locus_tag="ACHUU3_000465"
                     /gene_synonym="gcpE"
     CDS             complement(26045..27163)
                     /gene="ispG"
                     /locus_tag="ACHUU3_000465"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR2080638.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(27190..28203)
                     /gene="rodZ"
                     /locus_tag="ACHUU3_000466"
     CDS             complement(27190..28203)
                     /gene="rodZ"
                     /locus_tag="ACHUU3_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR2080639.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(28488..29642)
                     /locus_tag="ACHUU3_000467"
     CDS             complement(28488..29642)
                     /locus_tag="ACHUU3_000467"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR2080640.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(29792..30223)
                     /gene="ndk"
                     /locus_tag="ACHUU3_000468"
     CDS             complement(29792..30223)
                     /gene="ndk"
                     /locus_tag="ACHUU3_000468"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR2080641.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(30372..32684)
                     /gene="pbpC"
                     /locus_tag="ACHUU3_000469"
     CDS             complement(30372..32684)
                     /gene="pbpC"
                     /locus_tag="ACHUU3_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR2080642.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(32685..37646)
                     /gene="yfhM"
                     /locus_tag="ACHUU3_000470"
     CDS             complement(32685..37646)
                     /gene="yfhM"
                     /locus_tag="ACHUU3_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFR2080643.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            37853..38698
                     /gene="sseA"
                     /locus_tag="ACHUU3_000471"
     CDS             37853..38698
                     /gene="sseA"
                     /locus_tag="ACHUU3_000471"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFR2080644.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            39034..39159
                     /gene="timP"
                     /locus_tag="ACHUU3_000472"
     CDS             39034..39159
                     /gene="timP"
                     /locus_tag="ACHUU3_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR2080645.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(39191..39967)
                     /gene="sseB"
                     /locus_tag="ACHUU3_000473"
     CDS             complement(39191..39967)
                     /gene="sseB"
                     /locus_tag="ACHUU3_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR2080646.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(40109..41392)
                     /gene="pepB"
                     /locus_tag="ACHUU3_000474"
     CDS             complement(40109..41392)
                     /gene="pepB"
                     /locus_tag="ACHUU3_000474"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR2080647.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(41570..41770)
                     /gene="iscX"
                     /locus_tag="ACHUU3_000475"
     CDS             complement(41570..41770)
                     /gene="iscX"
                     /locus_tag="ACHUU3_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR2080648.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(41782..42117)
                     /gene="fdx"
                     /locus_tag="ACHUU3_000476"
     CDS             complement(41782..42117)
                     /gene="fdx"
                     /locus_tag="ACHUU3_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR2080649.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(42119..43969)
                     /gene="hscA"
                     /locus_tag="ACHUU3_000477"
     CDS             complement(42119..43969)
                     /gene="hscA"
                     /locus_tag="ACHUU3_000477"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR2080650.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(43986..44501)
                     /gene="hscB"
                     /locus_tag="ACHUU3_000478"
     CDS             complement(43986..44501)
                     /gene="hscB"
                     /locus_tag="ACHUU3_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR2080651.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(44597..44920)
                     /gene="iscA"
                     /locus_tag="ACHUU3_000479"
     CDS             complement(44597..44920)
                     /gene="iscA"
                     /locus_tag="ACHUU3_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR2080652.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(44937..45323)
                     /gene="iscU"
                     /locus_tag="ACHUU3_000480"
     CDS             complement(44937..45323)
                     /gene="iscU"
                     /locus_tag="ACHUU3_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR2080653.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(45351..46565)
                     /gene="iscS"
                     /locus_tag="ACHUU3_000481"
     CDS             complement(45351..46565)
                     /gene="iscS"
                     /locus_tag="ACHUU3_000481"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR2080654.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(46806..47294)
                     /gene="iscR"
                     /locus_tag="ACHUU3_000482"
     CDS             complement(46806..47294)
                     /gene="iscR"
                     /locus_tag="ACHUU3_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR2080655.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(47595..48332)
                     /gene="trmJ"
                     /locus_tag="ACHUU3_000483"
     CDS             complement(47595..48332)
                     /gene="trmJ"
                     /locus_tag="ACHUU3_000483"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR2080656.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            48451..49254
                     /gene="suhB"
                     /locus_tag="ACHUU3_000484"
     CDS             48451..49254
                     /gene="suhB"
                     /locus_tag="ACHUU3_000484"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR2080657.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            49399..50253
                     /locus_tag="ACHUU3_000485"
     CDS             49399..50253
                     /locus_tag="ACHUU3_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR2080658.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            50444..51724
                     /gene="csiE"
                     /locus_tag="ACHUU3_000486"
     CDS             50444..51724
                     /gene="csiE"
                     /locus_tag="ACHUU3_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR2080659.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(51716..52855)
                     /locus_tag="ACHUU3_000487"
     CDS             complement(51716..52855)
                     /locus_tag="ACHUU3_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR2080660.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     SAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(53015..53905)
                     /gene="hcaR"
                     /locus_tag="ACHUU3_000488"
     CDS             complement(53015..53905)
                     /gene="hcaR"
                     /locus_tag="ACHUU3_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR2080661.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            54041..55402
                     /gene="hcaE"
                     /locus_tag="ACHUU3_000489"
     CDS             54041..55402
                     /gene="hcaE"
                     /locus_tag="ACHUU3_000489"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR2080662.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            55399..55917
                     /gene="hcaF"
                     /locus_tag="ACHUU3_000490"
     CDS             55399..55917
                     /gene="hcaF"
                     /locus_tag="ACHUU3_000490"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR2080663.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            55917..56237
                     /locus_tag="ACHUU3_000491"
     CDS             55917..56237
                     /locus_tag="ACHUU3_000491"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR2080664.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            56234..57046
                     /gene="hcaB"
                     /locus_tag="ACHUU3_000492"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             56234..57046
                     /gene="hcaB"
                     /locus_tag="ACHUU3_000492"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR2080665.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            57056..58258
                     /gene="hcaD"
                     /locus_tag="ACHUU3_000493"
     CDS             57056..58258
                     /gene="hcaD"
                     /locus_tag="ACHUU3_000493"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR2080666.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        14266 a        15782 c        14566 g        13752 t
ORIGIN      
        1 tctggcaact ggcgcacagt agcgcagaca attttccggc actgacggta agccatttta
       61 ttaccgccaa tctgctcccg gtgatgctgg gtaatattat cggcggtgca gtgctggtga
      121 gtatgtgtta tcgggctatt tatttacgtc aggaatcctg aaaaatcagc ccggcgaaac
      181 agttcgtcgg gctgaagatt attcttgcgc tatttgccca tcgggccagg cgtggatcac
      241 ggctttgatc agcgtcgcca atggaatagc gaagaatacg ccccagaatc cccacaaacc
      301 accgaagatc accaccgata aaataatcac cagcggatgc aggttaaccg cttcggagaa
      361 caacaccggt actaacagat taccgtccag cgcctgaata atcagataca ctgcgaagca
      421 gctccagaat tccgtgcctg caccaaactg gaatagcgcc acgccaacca ccggaatggt
      481 caccacaaat gcgccaatgt acggaataag taccgagaag ccgaccagca ccgccagcag
      541 cagcgaatag ttcagcccaa agagcaagaa ccccagccaa gtggcgatcc ccaccacgat
      601 catctccagc actttgccgc ggatatagtt ggtgatttgt tgattcatct ccttccacac
      661 ctgtcctgcc agtccacggt tgcgcggcag cacccgacga acggcgttca gcatctgctc
      721 tttgtctttc agcaggaaga agaccatcaa tggtaccagc accagataga cggcaatggt
      781 cagcaaaccg accagcgagg cgagggaaat tttcaccacc gaatcgccca tggtcagcat
      841 ccgactgcgc atattttcgg ccattgcatc aataatgccc gcgtccatta atgccggata
      901 gcggcgcggc aacgtggcgg caaagtcaga aagcttattg agcatccccg gcatatcgcg
      961 gatcaagtag atgccctgtt gccaggcgat aggcagtacc acgaacgcca tcagtagcaa
     1021 tataccgacg aaaaccacca atacaatcga cgtcgcccag cggcgggagc agccaataga
     1081 ttgcaggcgc acggttggcc attccagcaa ataggccagc acaatagcca ccagcaacgg
     1141 agcaagcagg ccactaaaga aaaagataat gccaaatccg gcaactaaaa taaccagcaa
     1201 ggcaatcgct tccgggtcgc taaaacggcg gcgataccat tgcatcaaca tttcgagcat
     1261 aaagaccttc cctgaacctc aagagcggga ttgcgatccg caattgtatc gaaatgtcac
     1321 aaaaaagact tcgcttttta tgacggattc aggaaactga aaagtcattt gagtgggcta
     1381 atcttcgccg ttacactcaa aggcggcgcg gtgggaacaa tatttcacag tatcggtcaa
     1441 atgactatct tcagaaatac aggatagagg ttatgttcag gcagttgaaa aaaaacctgg
     1501 ttgcaaccct cattgctgct atgaccattg gtcaggtagc cccggcgttt gccgacagcg
     1561 cagacacctt gccggatatg ggaacctccg caggaagcac gctttccatt ggtcaggaaa
     1621 tgcagatggg cgactattat gtccgccagc tacgcggcag cgcgccgtta attaatgacc
     1681 cgctgttaac gcaatatatt aattcgctgg ggatgcgtct ggtttcgcat gccaattcgg
     1741 ttaagacacc gtttcatttt tttctgatca acaacgacga aattaacgcc tttgctttct
     1801 ttggcggcaa cgtggtgctg cactctgccc tgttccgtta ttccgataac gaaagtcaac
     1861 tggcttcagt tatggcgcac gaaatctccc acgtcaccca acgtcacctg gcgcgagcga
     1921 tggaagatca gcagcgcagc gcgccgctga cctgggtcgg cgcgttaggt tctattttac
     1981 tggcgatggc cagtccgcag gcggggatgg cggcgctgac cggtacactg gcgggaacgc
     2041 gtcaggggat gatcagtttc acccagcaaa atgaacagga agcggaccgc attggtattc
     2101 aggtgctgca acgctcggga ttcgatccgc aggcgatgcc aaccttcctc gaaaaattac
     2161 tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt attgactcac ccgttgccgg
     2221 aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat gcgcccgatg gtggtgcagt
     2281 cgtcggaaga tttctatctg gcgaaagcgc gcacactggg gatgtataat tccggacgta
     2341 accagctcac cagtgatttg ctggatgaat gggcgaaagg aaacgttcgt cagcaacgag
     2401 cggcgcaata tggtcgtgct ttacaggcga tggaagccaa taaatacgac gaggcgcgta
     2461 aaacgctgca accgttactg gcggcagaac ctggcaacgc atggtatctc gatctggcta
     2521 ccgatatcga tcttgggcaa aacaaagcca atgaggcaat caatcgcctg aaaaatgccc
     2581 gtgatttgcg caccaatccg gtgttgcagc tcaacctggc aaacgcttat ctgcaaggcg
     2641 gtcaaccaca agaagcggcc aatattctga atcgctacac ctttaataat aaagatgaca
     2701 gcaacggctg ggatttactg gcacaggcgg aagccgcgct aaataaccgc gatcaggagc
     2761 tggctgcgcg agcagaaggt tatgcgctcg ccggacgact cgatcaggcc atttcgctgt
     2821 tgagtagcgc cagttcgcag gtgaaattag gcagccagca acaagcgcgt tacgatgcgc
     2881 gcatcgacca gttgcgccag ctgcaggaac gctttaagcc ttataccaag atgtaattcg
     2941 cattaaagga agaacaatga ccaaacaggt aaaaatctac cataacccac gctgttcaaa
     3001 gagccgggaa acgctgaacc tgctgaaaaa aaacggcgtg gagccagaag tggtactcta
     3061 ccttgagaca cccgccgatg cggcaacgct gcgcgatttg ctgaaaatgc tggggatgaa
     3121 tagcgcccgt gaattgatgc gccagaaaga ggatctttat aaagaactga acctggctga
     3181 cagttcgctt agcgaagagg cgctgattca ggcaatggtg gataatccga agctgatgga
     3241 gcgcccgatt gtggtcgcga atggcaaagc gcggattggt cgtccaccgg agcaggtact
     3301 ggaaatcgtg ggttaagatg tgccccatcg ctagttgaag cacattgccg gatgcgacgc
     3361 ttgccatgtc ttatccggcc tacgaatact atggattaac ttcgcgtagt tcggataagg
     3421 cgttcgcgcc gcatccgaca ataaacacct tatctacaac ttcagaattt ctttcacaaa
     3481 cggaatggtc agcttacgtt gcgcggtaat cgacgcacga tccaactgat ccaacgtcat
     3541 aaatagcgtg cgcatttctc tgtcgagccg cttcagcaag aaacgcccca catcttccgg
     3601 cagttcaaaa ccacgcaaac gcgcgcgtaa ctgtagcgcc tgcaacttat cttcatcaga
     3661 aagtggctgc aatttgtaga tctgccccca gtcgagtcgc gacgcgagat ccggtaatcc
     3721 cagattcaac tgccgcggtg gacgatcgcc ggtgatcaac aatcgtgttt tgcccgattc
     3781 cagaattcga ttgtagagat cgaaaatcgc catctcccac aactcatcgc ctgcaataca
     3841 ctcaatgttg tcgatacaga ccagcgacaa atgctccata ccgtcgagca cttccggaac
     3901 aaaccaggtg cgtttatcca gcgggacata gcccaccgca tcgccacgct gcgacaattc
     3961 cgcgcaagcc gcgtgcagca gatggctgcg ccccgcgcct tcgcgtgccc agagatagat
     4021 gtaaccgcta tgttcctgac gcagcacgtt ttgcagcgca gccagtaaag aggagttatc
     4081 ccccggccag aaacttgcaa aggtttcgtc gtcaggaaga taaagtggca aagagagctg
     4141 tgccggtgtg ttcagagata cctcaaccag gatttcacaa aatcgcgaga agtttaccac
     4201 agaatcccat gatgtttgaa ccgggcagca acactgcccg gtcggtacat tatttgtctg
     4261 ttatatccgc gtcttctgcg tccagcacca cttcttcagg gcgcagcaca ctaatcagtt
     4321 tgaagatcaa acttaagccg ataccgacga tggtcgccag cgccatacct ttcagctccg
     4381 ccgcgccaat gtttaccttc gcgccactga caccgatgat caaaatcacg gaagtcagga
     4441 tcaggttctg tgctttgttg taatccactt ttgattcgat caaaacacga ataccggaag
     4501 caccgatgac accataaagc agcagcgaaa cgccgcccat caccggcaat gggatcatct
     4561 ggatagcggc agccagttta ccgacgcagg aaagcaggat agcgaaaatc gccgccccac
     4621 cgataaccca ggtactgtaa acacgggtga tcgccatcac gccaatgttt tctccgtaag
     4681 tggtatttgg cgtagagcca aagaagccgg agatcacagt cgacaggccg ttagcaaaca
     4741 tcgaacggtg cagacctgga tcgcgcagca gatctttttt gacgatatta gccgttacta
     4801 ccaggtgccc tacgtgttcg gcaataacca ctaacgccgc tggcagaata gtcagaatgg
     4861 caaaccactc gaagcgcggc gtatagaggg ttggcagcgc aaaccagtga gcattaataa
     4921 tcggcgtggt atcgacaatt cccattgcga aagagagcgc gtaccccacc agcacgccaa
     4981 ttaaaatcgg gataattgcc aggaaaccac ggaacagcac ggaacctaaa accgtgaccg
     5041 ccagggtggt aatagagatg atgatggttt tggagtctgg cgtttgccct tcagccggga
     5101 gtaaacccgc cataccggca gctacgcccg ccagctccag accgatgacg gcaacgattg
     5161 cgcccattgc cgcaggtgga aacagcacgt ccagccagcc ggtccccgct ttcttcacga
     5221 taaaagaaac caggcagaac agcacgccgc acataataaa gccgcccagc gcgacttcat
     5281 accctaacgg caacagtaac aataccggtg aaataaaggc aaagctggaa ccaagataag
     5341 ccggaatttt ccctttacag atgaagagat acagcagcgt tccaataccg ttaaataaca
     5401 gtacagtcgc cgggttaata tgaaataaga cgggcaccag gacggttgca ccaaacatgg
     5461 cgaacaaatg ttgcaaacta agcgggattg tctgtaaaag tggcggtctt tcactcaccc
     5521 cgatagcacg gcgcgtcata gtattatcct ctgtattatg tgttataggc gctttactca
     5581 aaaaaaagcc gactcttaaa gtcggcttta attattttta ttctttattt cgtaccaaag
     5641 attttgtcac cggcatcgcc gaggcccgga ataatgtatc cgtgctcgtt cagtccctgg
     5701 tcaatcgatg cggtatacag ttcgacgtcc gggtgcgctt tttccagcgc agcgatacct
     5761 tctggcgcag ctaccagcac cagaactttg atgctgctgc agcccgcttt tttcagcagg
     5821 tcgatggtcg cgataacgga accaccggtt gccagcattg ggtcaacgat cagcgccata
     5881 cgctcatcga tgttagaaac cagtttctgg aagtacggta ccggctccag cgtttcttca
     5941 ttacggtaca taccgacaac gctgatgcgc gcgctcggaa cattttccag cacaccgtcc
     6001 atcatcccca gaccggcacg cagaattggc acaacggtaa ttttcttacc tttgatctgg
     6061 tcgatttcta ccgggccgtt ccagccttcg atagttactt tttccgtttc gaggtcggcg
     6121 gtcgcttcgt aagtcagcag gctacccact tcggaagcga gttcgcgaaa gcgcttggtg
     6181 ctgatatctt gctcacgcat cagtcccagc ttgtgtttga cgagtgggtg tttgacttcc
     6241 acgatcttca tactctttct cctttgaggg gcagccacaa aaaaaatcga cggattatac
     6301 ctcctttctt caaggcggca atattctttt cgttgacttt agtcaaaatg ataacggttt
     6361 gagataaagt tattttatat tcaaatggtt atgaaagaag attattccat ccgaaaacta
     6421 acctttgccc tggcacaagt cttctttcgc cgcgcgtctg gggaaaagac gtgcaaaaag
     6481 gttatgcaaa gcagtctcga aaacgtttgc tttccctgtt agaattgcgc cgaattttat
     6541 ttttctaccg caagtaacgc gtggggaccc aagcagtgac cgataaaacc tctcttagct
     6601 acaaagatgc cggtgttgat attgacgcgg gtaatgctct ggttggaaga atcaaaggcg
     6661 tagtgaagaa aacgcgtcgt ccggaagtga tgggcggtct gggcggcttc ggtgcgctgt
     6721 gtgcattgcc gcaaaaatat cgtgaacccg tgctggtttc cggcactgac ggcgtaggta
     6781 ccaagctgcg tctggcaatg gacttaaaac gtcacgacac cattggtatt gatctggtcg
     6841 ccatgtgcgt taatgacctg gtggtgcaag gtgcagaacc gctgtttttc ctcgactatt
     6901 acgcaaccgg aaaactggat gttgataccg cttcagcggt gatcagcggt atcgcggaag
     6961 gatgtctgca atcgggctgt tcactggtgg gtggcgaaac ggcagaaatg ccggggatgt
     7021 atcacggtga ggattacgat gtcgcgggtt tctgcgttgg cgtggtagaa aaatcagaaa
     7081 tcatcgacgg ctctaaagtc agcgacggcg atgtgctgat tgcactcggt tccagcggtc
     7141 cgcactcgaa cggctattcg ctggtgcgca aaattcttga agtcagcggt tgtgatccgc
     7201 aaaccaccga acttgatggt aagccattag ccgatcatct gctggcaccg acccgcattt
     7261 acgtgaagtc agtgctggag ttgattgaaa aggtcgatgt gcatgccatt gcgcacctga
     7321 ccggcggcgg cttctgggaa aacattccgc gcgtattgcc agataatact caggcagtga
     7381 ttgatgaatc ttcctggcag tggccggaag tgttcaactg gctgcaaacg gctggtaacg
     7441 ttgagcgcca tgaaatgtat cgcaccttca actgcggcgt cgggatgatt atcgccctgc
     7501 ctgctccgga agtggacaaa gccctcgccc tgctaaatgc caacggtgaa aacgcgtgga
     7561 aaatcggtat catcaaagcc tctgattccg aacaacgcgt ggttatcgaa taatgaatat
     7621 tgtggtgctt atttccggca acggaagtaa tttacaggca attattgacg cctgtaaaac
     7681 caacaaaatt aaaggcacca tacgggcagt tttcagcaat aaggccgacg cgttcggcct
     7741 tgaacgcgcc cgccaggcgg gtattgcaac gcatacgctc atcgccagcg cgtttgacag
     7801 tcgtgaagcc tatgaccggg agttgattca tgaaatcgac atgtacgcac ccgatgtggt
     7861 cgtgctggct ggttttatgc gcattctcag cccggcgttt gtctcccact atgccgggcg
     7921 tttgctgaac attcaccctt ctctgctgcc gaaatatccc ggattacaca cccatcgtca
     7981 ggcgctggaa aatggtgatg aagagcacgg tacatcggtg catttcgtca ccgatgaact
     8041 ggacggtggc ccggttattt tacaggcgaa agtcccggta tttgctggtg atacggaaga
     8101 tgacatcacc gcccgcgtgc aaacccagga acacgctatt tatccactgg tgattagctg
     8161 gtttgccgat ggtcgtctga aaatgcacga aaacgccgcg tggctggatg gtcaacgtct
     8221 gccgccgcag ggctacgctg ccgacgagta atgccccgta gttaaagcgc cagctctgcc
     8281 gctggcgttg caagctccag cagttttttt cccccttttc tggcatagtt ggacatctgc
     8341 caatattgct cgccataata tccaggcagt gtcccgtgaa taaaacggag taaaagtggt
     8401 aatgggtcag gaaaagctat acatcgaaaa agagctcagt tggttatcgt tcaatgaacg
     8461 cgtgcttcag gaagcggcgg acaaatctaa cccgctgatt gaaaggatgc gtttcctggg
     8521 gatctattcc aataaccttg atgagttcta taaagtccgc ttcgctgaac tgaagcgacg
     8581 catcattatt agcgaagaac aaggctccaa ctctcattcc cgccatttac tgggcaaaat
     8641 tcagtcccgg gtgctgaaag ccgatcagga attcgacggc ctctacaacg agctgctgct
     8701 ggagatggcg cgtaaccaga tcttcctgat taatgaacgc cagctctccg tcaatcaaca
     8761 aaactggctg cgtcattatt ttaagcagta tctgcgtcag cacattacgc cgattttaat
     8821 caatcctgac actgacttag tgcagttcct gaaagatgat tacacctatc tggcggtgga
     8881 aattatccgt ggcgatacca tccgttacgc gctgctggag atcccatcag ataaagtgcc
     8941 gcgctttgtg aatttaccgc cagaagcacc gcgtcgacgc aagccgatga ttcttctgga
     9001 taacattctg cgttactgcc ttgatgatat tttcaaaggc ttctttgatt atgacgcgct
     9061 gaatgcctat tcaatgaaga tgacccgcga tgccgaatac gatttagtgc atgagatgga
     9121 agccagcctg atggagttga tgtcttccag tctcaagcag cgtttaactg ctgagccggt
     9181 gcgttttgtt tatcagcgcg atatgcccaa tgcgctggtt gaagtgttac gcgaaaaact
     9241 gactatttcc cgctacgact ccatcgtccc cggcggtcgt tatcataatt ttaaagactt
     9301 tattaatttc cccaatgtcg gcaaagccaa tctggtgaac aaaccactgc cgcgtttacg
     9361 ccatatttgg tttgataaag cccagttccg caatggtttt gatgccattc gcgaacgcga
     9421 tgtgttgctc tattatcctt atcacacctt tgagcatgtg ctggaactgc tgcgtcaggc
     9481 ttcgttcgac ccgagcgtac tggcgattaa aattaacatt taccgtgtgg caaaagattc
     9541 acgtatcatc gactcgatga tccacgctgc gcacaacggc aagaaagtga ccgtggtggt
     9601 tgagttacag gcgcgtttcg acgaagaagc caacattcac tgggcgaagc gcctgaccga
     9661 agcaggcgtg cacgttatct tctctgcgcc ggggctgaaa attcacgcca aactgttcct
     9721 gatttcacgt aaagaaaacg gtgaagtggt ccgttacgca cacatcggga ccgggaactt
     9781 taacgaaaaa accgcgcgtc tttatactga ctattcgttg ctgaccgcag atgcgcgtat
     9841 caccaacgaa gtacggcggg tatttaactt tattgaaaac ccataccgcc cggtgacatt
     9901 tgattattta atggtgtcac cgcaaaactc tcgccgtctg ttatatgaaa tggtagaccg
     9961 cgaaatcgcc aacgcgcagc aagggctgcc cagtggtatc accctgaagc taaataacct
    10021 tgtcgataaa ggcctggttg atcgtctgta tgcggcctcc agctccggcg taccggttaa
    10081 tctgctggtt cgcggaatgt gttcgctgat ccccaatctg gaaggcatta gcgacaacat
    10141 tcgtgccatc agtattgttg accgttacct tgaacatgac cgggtttata tttttgaaaa
    10201 tggcggcgat aaaaaggtct acctttcttc cgccgactgg atgacgcgca atattgatta
    10261 tcgtattgaa gtggcgacac cgctgctcga tccgcgcctg aagcagcggg tgctggacat
    10321 catcgacata ttgttcagcg atacggtcaa agctcgttat atcgataaag aactcagtaa
    10381 tcgctacgtt ccccgcggca atcgccgcaa agtacgggcg cagttggcga tttatgacta
    10441 catcaaatca ctcgaacaac ctgaataacc ctatgccaat acacgataaa tcccctcgtc
    10501 cgcaggagtt tgctgcggtc gatcttggtt caaacagttt tcacatggtc atagcccgtg
    10561 tggtagatgg tgccatgcag attattggcc gcctgaaaca gcgggtgcat ctggcggacg
    10621 gcctggggcc agataatatg ttgagtgaag aggcaatgac gcgcggtttg aactgtctgt
    10681 cgctgtttgc cgaacggcta caagggtttt ctcctgccag cgtctgtata gtcggcaccc
    10741 atacgctgcg tcaggcgctg aacgccactg actttctgaa acgcgcggaa aaggtcattc
    10801 cctacccgat tgaaattatt tccggtaatg aagaagcccg tctgattttt atgggcgtgg
    10861 aacataccca accggagaaa ggtcgcaaac tggttattga tattggcggc ggatctacgg
    10921 aactggtgat tggtgaaaat ttcgaaccga ttctcgttga aagccgccgg atgggttgtg
    10981 tcagctttgc ccagctttac ttccctggcg gggtcatcaa taaagagaat tttcagcgcg
    11041 ctcgtatggc ggcagcacaa aaactggaaa ctttaacctg gcaattccgt attcagggct
    11101 ggaacgtggc aatgggcgct tccggtacca taaaagccgc ccatgaagtg ttaatggaaa
    11161 tgggcgagaa agacgggata attaccccgg aacgtctgga aaaactggta aaagaagttt
    11221 tacgtcaccg taatttcgca tcgctgagtt taccgggtct ttccgaagag cggaaaacag
    11281 tcttcgttcc tggactggcg attttatgcg gtgtgtttga tgctttagcc atccgtgaac
    11341 tgcgcctttc tgacggggcg ttacgcgaag gcgtactgta cgaaatggaa ggacgtttcc
    11401 gtcatcagga tgtgcgtagt cgcaccgcca gcagcctcgc caaccagtat cacatcgaca
    11461 gcgaacaggc ccgacgggtg ctggatacca ctatgcaaat gtacgaacag tggcgggaac
    11521 agcaaccgaa gctggcgcat ccgcaactgg aggcgctact gcgatgggcc gccatgctgc
    11581 atgaggtcgg gttgaatatc aaccacagcg gtttgcatcg ccactccgct tatattctgc
    11641 aaaacagtga cttgccgggt tttaatcagg aacagcagct gatgatggcg acactggtgc
    11701 gctatcaccg taaagcgatt aaactcgacg atctgccgcg ctttaccttg tttaagaaga
    11761 aacagttcct gccactgatt cagctattgc gccttggcgt attactcaac aatcaacgtc
    11821 aggcaaccac cacaccgcca acattgacac tgattactga tgacagtcac tggacactgc
    11881 gtttcccgca tgactggttt agtcagaatg cgttggtact gcttgatctg gaaaaggagc
    11941 aagaatactg ggaaggcgtg gctggctggc ggttgaaaat tgaagaagaa agtacacctg
    12001 aaatcgccgc ttaatgcttt gcgggccgac gagaaatgtc ggcccgcatt attcaggcac
    12061 tttcgcgaat gggttcgatt tcattcaacg tatcaattaa cggctgcggc ttaccaataa
    12121 gataaccctg catataatcg atccccaaag agagcaccgc ctcgcggatc tcttcgtttt
    12181 caacgtactc tgccactacc cgcattttct tcattcgcgc cagatggcaa atcgacgcca
    12241 caatctgata atccagacta tttgacacaa tattgcggat aaagctgccg tcaattttaa
    12301 gcagatcggc attcacattt ttaagccgtg catagctggc gtagccggtg ccgaaatcat
    12361 caatcgcaat ctggcagcct aattcctgaa gatgctgcaa ggtgatttgc gcctgcttaa
    12421 cattggtcag agcattactt tcggtgactt caaaaataag ttgccacgct tcaatctgat
    12481 atttagccag caactgactg acttcaacag gaaaacgagc ctgacatacc gaggttggag
    12541 acagattaat agcaaaacgg tgagcgggca tcttcgctct gttttcagcc ataaattgca
    12601 gcgtatgctc aatgacccac atgtcgatac tcgacgataa accaaattcg tgcgcgaccg
    12661 gcaagaagct atcggggacg atcagttcat cattctcacc tttcatgcgc agaagaattt
    12721 catggtaaac atcaccacgc ataccggtaa tcggctgggc catcaggaaa aaatggttgt
    12781 gttccagcgc ctgctgtagt cgattcatca tcgcgacttt atctttcaat tcgcgttgca
    12841 aatacattgc cccgcgacgc tgcatatttt ccggggcgtt ggtcacgatg gaaagttcgg
    12901 cgaccgtatt tagctctccc agcagcaggt agatatgatt cactggcgag cgcacatagc
    12961 agtaactgac gccaatctgc ggttgcatcg gcatcccatc ccaaaagaaa cgaaattgct
    13021 tgagatgact atccagtgcg gtaatgcgct cctggtgcga ttctgtattc agtcgcagcg
    13081 cgagatcgtt acccgaaagc tgataaacat cttcacccgg ttccagcaag ggtgacagcc
    13141 agtgagaaag tttttgcttg tattgaatcc gcagcatgat gccatagttc ttaaccagca
    13201 tttccatgcc agggatgcgt aaataacaaa gcgcagacca gggagcatca cgcaacgcgc
    13261 gattcagggc gcgaacattt ggcagatgaa ccaccgggtc catatacgca agccgctgaa
    13321 tgcgtctgac taccgctctc tgacgggttg ccagtacagc catataattg acaataaaag
    13381 agaataccag ataactggag gaggttatgg tcagctgcgt ggtatagcca ggataaatgg
    13441 gaatgtaatt ttgatagctg tggatgctga tcatcaacac gaccgcccag agcagcgaaa
    13501 tcagcttata accatagcgc atcgctcccc acatcatcag gggcagcaat aatgacaagg
    13561 tataattggt gctaaaaatt gtgctttttt catttaacgg catgcataac agcaacaata
    13621 aagcacctaa tgccagtagc cagagcgcga actctttttt ggtgactttg gcatcaacct
    13681 gctgttttaa ttgcgaataa tagctacgta aataaaatgg atttcgcact acccgaatga
    13741 tgaagtagca cagcgggaca ccgatcagat tacccaccag taaggcctga taattgatta
    13801 aagtcccgag gttaaaaggc atgacaccga ccagattttc tctgctcgcc agtaatccta
    13861 caaatgcagc aaactggaaa agtatcagaa acagcgttgc aggaaacaca atctgccaga
    13921 atatacgctg ggaaattaaa cgggtatcac catgtgaaac gttgttacgc cggggagtaa
    13981 agaccctgta accgccccaa cagagaatga taatgataat aaaattagcc gttaatgata
    14041 gcgtttcgta aaaacccagc tctgcatatt tgcggacaaa aatccctaag gcgatccctg
    14101 gcaatgccgc ccagctgaaa aacatcatca tgctgatcat cagtgccaga ggcagataaa
    14161 agagaaaaac ctcaccggaa gaaatatgcg cgaaagtatt aatgtgggcg aaaatgggca
    14221 agattaaaga aggcaggaac agcggaagcc cccaccattt atcacgcatt tttatataag
    14281 ttgcattcag tttcatagat gctcagcaga atcccccaca tcctgaagga ggtgtattca
    14341 gacaggcatc ccacctgact tcgaatgatg attattcatc actatagaga gcattgattc
    14401 taagtgtcat atgaaagttc caattgatat atatcaaaca aaataaccct gattaatgaa
    14461 ttattacgtt tatcatgtta attcatcatt attacatcat cattgtaaat aattaaatta
    14521 acttccataa cattaaaata tgtatccact gacgtttttt tacataacga agaattgacc
    14581 attttgtcct gttgtgcctt aatgtaagta ccgtccacag cgtgggacat acttcaagga
    14641 accttttgtg agtcaggcaa ccagtatgcg aaaacgacac cgatttaaca gtcgcatgac
    14701 ccgtatcgta ctgctcatca gctttatctt cttctttggc cgttttatct actcgtccgt
    14761 cggtgcctgg cagcaccatc agagcaaaaa agaagctcag caatccacac tctccgtcga
    14821 atcaccggta caacgttagc ggttaccttc ttcactttca cagaacataa cggcacttcg
    14881 ctgtcggatg cttttgctct ttgggattat caaagcggca gatattcttt catcttaaat
    14941 tttacgtctt tgtcctgact gatgtttatc ctgtttggct gcggaataaa tataaaatta
    15001 atatatatgt tgtaatgata tatttttata aattattccc tgcgtgaatt ttaataaatt
    15061 taatctatcc ctttatacgc aatacattta ctttcctctt ttgatgatct taaatgtctt
    15121 atttttcgta atgtgtataa caaggaatag tgatgaaatt taaaaaatgt cttctgcctg
    15181 tggcaatgtt agcgtcattc actctggcag gatgccagtc aaatgctgac gatcatgccg
    15241 ccgatgttta tcaaaccgat caactgaata ccaaacaaga aactaaaacc gttaatatta
    15301 tttccattct tcccgcaaaa gttgccgtag acaactccca aaataaacgg aacgcacaag
    15361 ccttcggcgc gcttattggc gcagtcgctg gcggtgttat cggccacaac gtcggttctg
    15421 gcagcaattc cggaacgacg gcaggggcag ttggcggcgg agctgtaggc gcggcagcgg
    15481 gttctatggt gaatgataaa accttagtgg aaggtgtttc tttaacatat aaggaaggca
    15541 ccaaagtgta tacctccacc caggtgggta aagagtgcca gtttacgaca ggtttagccg
    15601 ttgttattac cacaacgtat aacgaaacgc gtattcagcc aaatactaaa tgtcctgaaa
    15661 agagctaata atcaggagga gtcatgaaga aagtttttct ttgcgccatc ttagcctcct
    15721 taagctatcc ggctatcgcc tcatcattgc aggatcaact ctctgctgtc gcagaagcgg
    15781 aacagcaagg taaaaatgaa gagcaaaggc agcatgacga atgggtcgcg gagcgcaaca
    15841 gggaaatcca gcaagagaag caacgtcgcg caaatgccca ggccgccgct aacaaaagag
    15901 cggcaacggc agcggcaaat aagaaagctc gtcaggataa actggacgcc gaagccactg
    15961 cggacaaaaa acgcgatcaa agttatgaag atgagctacg cagcttagag attcagaaac
    16021 aaaaactgac gctggcgaaa gaagaagccc gcgttaagcg agaaaacgaa tttatcgatc
    16081 aggaactgaa gcacaaagct gcgcaaaccg atgtggtgca atctgaagct gacgccaaca
    16141 gaaatatgac tgaaggcggt cgcgatctga tgaaaagcgt gggcaaagca gaagagaaca
    16201 aatcggacag ctggtttaat taatcgatgt tagtaacttc aagcctatga ttcttgaaga
    16261 taaaaaaccc tctgtagtaa cagagggttt tgttcattca tagtgcaggg attaaaatca
    16321 ttcccactca atggtagctg gcggcttgcc gctgatgtca tacaccacgc gggaaatacc
    16381 gttcacttca ttgataatgc ggttggaaac gcgaccgagg aaatcgtacg gcagacgcgc
    16441 ccagtgtgcg gtcataaagt cgatggtttc gacagcacgc agagagacaa cccagtcata
    16501 cttacgacca tcgcccatta cgccaacgga acgtaccggc aggaacacag tgaacgcctg
    16561 gctgactttg tcgtacaggt ccgctttacg cagttcttca atgaagatgg cgtcagcacg
    16621 gcgcagcagg tcacagtact ctttcttcac ttcacccagc acacgaacgc caaggcctgg
    16681 tcccgggaat gggtgacggt acaacatgtc gtacggcagg cccagctcca gaccaatctt
    16741 acgcacttcg tctttgaaca gctctttcag cggttcaacc aggcccatct tcatctcttt
    16801 cggcaggccg cccacgttgt ggtgagattt gatgacgtgt gctttaccgg ttgcagacgc
    16861 cgcagattcg ataacgtcag ggtagatggt gccctgcgcc agccacttca cgtcttccag
    16921 tttcagcgct tcttcatcga atacttcaac gaaaacgcga ccgatgattt tacgttttgc
    16981 ttccggatcg ttttcgccag ccagcgctga caggaagcga tcttctgccg gaacgtgaac
    17041 aatgttcaga ccaaagtgat cgccaaacat atccagaacc tgctctgctt cgttgagacg
    17101 cagcaggccg ttgtcgacga atacgcaagt caggttttta ccgatagcgc ggtgcagtag
    17161 cattgcggtt acggaggaat ccacgccacc agagaggccg aggatgactt tatcgtcgcc
    17221 tacctgctca cggatgcggg ctacagcatc gtcgataatt ttcgctggcg tccacagggc
    17281 ttcacactgg cagatatcac gcacaaaacg ctccagcatg cgcataccct ggcgggtgtg
    17341 agtcacttcc gggtggaact gtacgccata gaagcgtttt tcttcgttag ccataatggc
    17401 aaacgggcag ctttcggtgc tggctacggt gacgaagtcg gacgggatag cggtaacttt
    17461 atcgccgtgg ctcatccaga catcgagcag cggtttaccg tctgcggtca gcgcatcttc
    17521 gataccgcga accagtgcac tgtcgtttac gacttcaacc tgcgcgtagc caaattcacg
    17581 ttcgttagag gcttcaacgt gaccgcccaa ctgcattgcc atggtctgca tgccatagca
    17641 aacgccgaat accggtacgc ctgcttcaaa gacatactgc ggcgcacgcg ggctgttttc
    17701 ttcagtagta ctttccgggc cgccggaaag aataatgccg cttggattga agtcacgaat
    17761 ttgtgcttct gtcacatccc acgcccacag ttcgcagtaa acacccagct cacgcacgcg
    17821 gcgcgcaacc agttgagtgt actgagaacc gaagtccaga atgaggatgc gatgcttatg
    17881 aatgttttcc gtcattgacg cttattccga ggcaagtgaa acagataata taaatcgccc
    17941 gacatgaagt cgggcgaaga gaatcaggag cccagacggt agttcgggga ctctttagta
    18001 atggtcacgt cgtgaacgtg gctttcctga atgcccgcac cgctgatacg tacaaactcc
    18061 gctttagtac gcagttcgtc gatagtacca cagccggtca gacccataca ggagcgcagg
    18121 ccgcccatct gctggtgaat gatctctttc aggcgacctt tataagctac gcgaccttcg
    18181 ataccttccg gcaccagttt gtcggcagcg ttatcgctct ggaaataacg gtcagaggaa
    18241 cctttggaca tcgcgcccag ggaacccata ccacggtaag atttgtaaga acggccctgg
    18301 tagagttcga tttcacccgg agattcttca gtacccgcca gcatggaacc taccatcacc
    18361 gcgcttgcgc cagcggcgat agctttggcg atgtcgccgg agaagcgaat accaccatct
    18421 gcgataaccg gaataccggt gccttccagg gcttcaactg cgtcagcaac ggcagtgatc
    18481 tgcggaacac ctacgccagt aacgatacga gtcgtacaga tagaaccagg gccgataccg
    18541 actttaaccg cgctgcaacc agcttctgcc aaagcgcgtg cacctgcagc tgttgccacg
    18601 ttgccgccga tgatttgcag atccggatat ttagcacggg tttcacggat acgctgcaaa
    18661 acgccttctg agtgaccgtg ggaggagtcg atcagcagaa cgtcaacgcc agcggcaacc
    18721 agcgcgtcaa cacgctcttc gttacccgca cctgcgccaa ctgcagcacc aacacgcaga
    18781 cggccttgct cgtctttaca ggcgttcggt ttacgttccg ctttctggaa gtctttcacg
    18841 gtgatcatgc cgatcaggtg gaattcgtca tcaaccacca gcgctttttc aacgcgtttt
    18901 tcgtgcattt ttgccagcac cacttcacgg gcttcacctt cacgcacggt gaccagacgc
    18961 tctttcggcg tcatgtaaac gctaaccggc tggttcaggt cggtaacaaa acgcacgtca
    19021 cgaccggtga taatacccac cagttcgttt tcttcggtaa cgaccggata gcccgcaaaa
    19081 ccgttacgct cggtcagttc tttcacttcg cgcagcgtcg tggttggcag aacagtctgc
    19141 ggatcagtca ccacaccaga ttcgtgtttt ttcacacggc gaacttcttc tgcctggcgt
    19201 tcaatggaca tgtttttgtg gataaagccg ataccgcctt cctgagccag agcaatagcc
    19261 aggcgcgctt ccgttacggt atccattgct gcggaaagca tagggatatt cagacgaata
    19321 gttttcgtca gctgggtgct gaggtcagca gtattcggca gaacggtaga atgagcagga
    19381 acgaggagaa cgtcgtcaaa cgtcagagct tctttagcga tacgtagcat gggcaatatc
    19441 tcgaccagag tggttaataa atattgccgc ggcattatac agagcgtaac cgattgcatc
    19501 tacccctttt tgcaaaaaat gcttgctatc cccgaagggc gggttactat cgactgaata
    19561 acctgctgat ttagaatttg atctcgctca catgttacct tctcaatccc ctgcaatttt
    19621 taccgttagt cgcctgaatc aaacggttcg tctgctgctt gagcatgaga tgggacaggt
    19681 ttggatcagc ggcgaaatct ctaatttcac acaaccggct tccggtcact ggtactttac
    19741 actcaaagac gacaccgccc aggtacgctg cgcgatgttc cgcaacagca accgccgggt
    19801 gaccttccgt ccacagcatg gacaacaagt tttagttcgc gccaatatta cgctctacga
    19861 gccgcgcggt gactaccaga taatcgttga gagtatgcag ccggccggtg aagggctgct
    19921 gcaacagaag tacgaacagc tcaaagcgaa gttgcaggct gaaggtttgt tcgatcagca
    19981 atacaaaaaa ccacttccct cccctgcgca ttgcgttggt gtgatcacct caaaaaccgg
    20041 tgctgcgcta catgatattt tgcatgtgtt aaaacgtcgc gatccttctc taccggtgat
    20101 catctacccc accgccgttc agggcgatga cgcaccgggg caaattgttc gcgccattga
    20161 actggcgaat cagcgcaatg agtgcgacgt gttgatcgtt gggcgcggcg gcggttcgct
    20221 ggaagattta tggagtttta acgacgaacg cgtagcgcgg gcgatttttg ccagccgcat
    20281 tccggtcgtc agcgccgtcg ggcatgagac ggatgtgacc attgccgatt ttgttgccga
    20341 tctgcgtgcg ccaacgccgt ctgccgccgc tgaagtagtg agccgtaatc agcaagagtt
    20401 actgcgccag gtgcaatcga cccgtcaacg gctggagatg gcgatggatt attatctcgc
    20461 caaccgcacg cgtcgcttta cgcagatcca tcaccgatta cagcaacagc atccgcagct
    20521 ccggctggca cgccagcaaa ccatgcttga gcgcctgcaa aagcggatga gctttgcgct
    20581 ggaaaatcaa cttaagcgta ccgggcaaca gcagcagcgg ttaacacagc ggctgaatca
    20641 gcaaaatcca cagccgaaga ttcatcgcgc gcaaacgcgc attcagcaac tggaatatcg
    20701 tttagcagaa accctgcgcg tacagcttag cgccacgcgt gaacgtttcg gtaatgcagt
    20761 aacgcacctc gaagccgtaa gcccactgtc aacgctggcg cgtggataca gcgttactac
    20821 tgctactgac ggcaatgtac tgaaaaaagt gaagcaagtt aaagcgggtg aaatgctaac
    20881 cacacgtctg gaagacggct ggatagaaag tgaagttaaa aacatccagc cagtaaaaaa
    20941 atcgcgtaaa aaggtgcatt aagccagcac gaattcaacg cgttttttcg atatcaagcc
    21001 atgcccgtgc tgacagaagt aatctactgc accgcaggct tttaacacct gcaacggttg
    21061 atggcagtct ggacaaagag ctttcatttc aataatttcc ccgcaggatg ggcaacgcgc
    21121 atggccgtta tcctgatcaa gcacatgctg acattgtgga caatgcagtt ccatatgaat
    21181 tcctccggta gccatccctc tacattcata gagggaatgg cagataaaat acttacggat
    21241 aattatttat ttttcttgat gtgcttcatc agacgcttac gtttacgcat ctgggttggc
    21301 gtcagggtgt tacgcttgtt cgcatacggg ttttcccctt ctttgaactg aatacgaatc
    21361 ggcgatccca ttacgtccag cgatttgcgg aagtagttca tcaagtagcg cttgtaggaa
    21421 tcaggcaggt ctttcacctg attaccgtga atcaccacaa tcggcgggtt ataaccacca
    21481 gcgtgggcat atttcagctt cacacgacga ccgcgtacca gcggcggttg gtgatcttca
    21541 acagccatcg tcatgatgcg cgtcagcata gaggtcccca cacgacgggt ggagctgtca
    21601 tacgcttcac gtactgattc aaacaagtta ccaacaccac tgccgtgcaa ggcagagata
    21661 aagtgcacac gagcaaaatc gataaagccc agacggaagt ccagcgtttc tttcacctgc
    21721 tctttcactt cctggctcag gccatcccac ttattcacca caatgacaag tgagcgccca
    21781 ctattgagaa taaagcccag cagcgagaga tcctgatcgg aaataccttc gcgcgcatca
    21841 atcactaaca tcaccacgtt ggtatcttca atggcctgca acgttttgat tacggagaat
    21901 ttctctacag catcggtgat tttgccgcgt ttacgtacgc cagcggtgtc aatgagcaca
    21961 tactcacgtc catcgcgttc cattggaatg tagatactgt cacgcgtcgt gccaggcatg
    22021 tcataaacaa caacgcgctc ttcaccaaga atacggttag tgagtgtaga cttacctacg
    22081 ttcggacgac ccacaatcgc cagtttgatc ggcagacttt gcgggtcgaa gtcgtcttcc
    22141 tcttcttctt cgccgttctc ttccgcttca aattgcgccc agtattcagc gtcttcgtcg
    22201 acttcctctt gcggtgcgag atcttccatc cacggcagca gcacacgctc cagcagactt
    22261 aatacgccac gaccgtgaga tgcggcgatc gggtagattt cacctaaacc aagcgagtag
    22321 aaatcaacca ctgcctgatc gggatccaga ccgtcagttt tgtttgccac caggaaggtc
    22381 ggtttttcac gagagcgcag atgtttggca atcgcttcat ctgccggcat caagcccgcg
    22441 cgcgcatcca ccataaacag tacgacgtcc gcttcttcaa tcgccagcag cgactgttcc
    22501 gccatgcggg tttctacacc gtcttctgtg ccatcaatcc cgccggtatc aatacagata
    22561 aactcacggc cttcaatttc cgcacgaccg tacttacggt cacgagtcag acccgggaaa
    22621 tccgcaacca gcgcatctcg ggtgcgagtt agacggttaa ataacgtgga ttttcctacg
    22681 ttagggcgcc cgacaagcgc gaccacaggt accatgttta aagcctcatt tttataaatc
    22741 atcagacaac gcacgctata ttcgcgtcgt tgttaaaaac aggaaaacgg cccctgtcca
    22801 ggagccgttt tcaaagtgaa cgacagagac gattaacgtg taatagagta cacggttccg
    22861 tcttttgcct ggatcagcag tttgccgtca gcggcaaccg gttcagtctg gaaaccggaa
    22921 ctatcaactt tttgctgggc aacgaaacga ccatcttcga cgttaatcca gtgcagataa
    22981 ccttcactgt cacctaccac caggttgcca ttatacagca ccggcgaagt cagcaggcga
    23041 tgcagcagat cgctttgtgt ccacagcgta acgccgccat caatggtcaa cgccatcacc
    23101 cggtcatttt gatcgaccag atagatgcga ttgccgtcga cgatgaaatc attcaccgaa
    23161 cccagttcgc gtttccacat aatctgacca ctgcgcagat caagcgccgt caggttacca
    23221 ttataggcca gagcgaaaac aacgccgtta acaacgacgg gagtcgtgtc aacatcgctc
    23281 agacggtcaa tttcggtaga accggtcgcc tgggaaatac gctgctgcca aatcatctgg
    23341 ccctgttcca tcagcactgc gctgacgcga ccattatcgc cccccacgac ggccgcacca
    23401 aaagccgttg ccggcgcaga ctcgccacgc aaagagagcg aaggcatatc gaggttaact
    23461 gtccatttga cagcgccgtc agcttcgttc agcgcttgta actgaccgtt actagtgtgg
    23521 attaacacca gaccgtcgct gaccaccggg cgagaaagtg cttcacccgc gactttagtt
    23581 tgccatgcca cagtaccatc gctggtattc agcgcgtaaa cctgcgcctt ttcgctgcca
    23641 atgtagacat gcccaccaga cacggtcaca ccgccagaaa gtaatgcagg ctctttagag
    23701 aaccagccat ctttctcggc caggctgaca gaccagattt ctttgccatc atccgcattc
    23761 agcgctttta ccaaaccagc gcggtccgct gcatagacaa cgttgtccgc cagtgccgga
    23821 tgaagattgg aatagaagtt gccaatgccg ctaccaacgg aagtgctcca cgccgtggtc
    23881 ggcgtaaact ggttttcaac ggttggcaat ggggacatct ttaccacatc ttcttcgctg
    23941 ttaaacagcg aacagccgct taaaagggta acggaaagca gtcctggcag cagtaattta
    24001 cgcaattgca tcgggtccct ctcagatgga caaattatta attttcatct gcatcatttc
    24061 gctcagtgcc ggagtaacat cgcttttcac gcctgcttcc catgcactac gcgcaccttg
    24121 cttatcacct ttgctcaaca atgctttacc acgcaggtcg gcaacaatgg cagcccaccc
    24181 ttcacctttg atggtatcaa gggttttcag cgcggcatca gcctgcttga gctgtacctg
    24241 aacgcgagca agacgcagat ttatcacggc tttgagattt tcatcactcg tgtctgccag
    24301 cccctgttgt aactgggcgg cagctttctc tagttcattt ttgtcaacaa attgctgcgc
    24361 aagttccaaa gaagccagcg caccataagt atttttattt tcagcagcaa atttttccgc
    24421 cgccgggatg ctatccggtt tgccttcgct cactgcggta accgcatttt gataggcaag
    24481 agaagcggag cgtgcagaat caacctgatg gctgttccag tagcgccagc caatcagtgc
    24541 gccaacgccc aaaatcaccc caacagccag tgctttgcca ttttcagcaa aaaagcgttt
    24601 aaccgcttct acctggtcgt tttcgttctc gtaaatttcc acgctgtcct tctccttcct
    24661 taacccagta acgtgcgcaa atgcgcggct acgctatcct gcgcaactgc cgtttgctca
    24721 ccagagcgca aatccttcac tactgctgtg ccgttagcca cttcagactc acccagcacc
    24781 acagcaacgc gggcacccca tttatcagca cgggcaaact gtttcttaaa gttgccgccg
    24841 ccgtggttgg tcatcaattt cacgcccggt aattcatcac gcagacgctc agctaatgcc
    24901 atagccgcag attgtgtatc agcacctgaa gccaccaggt atatatcgac aacaggatcg
    24961 gctttaaatt ccggattaac ggcctgtact aacaatacaa gacgttcgag gcccatcgca
    25021 aaaccgactg ccggtgttgc acgaccgccc agttgttcca ccagaccgtc ataacgaccg
    25081 cctgcacaca cggtgccctg ggagccgagg ctgttagtca cccactcgaa aacggtacgg
    25141 ttatagtaat ccagaccacg caccagacgc tggtttacgg tgtaagcgat ccccgcgctc
    25201 tcaagcagtt tgcacagacc ggcaaaatgc tcacgagatt cctcgtccag ataatcacct
    25261 aatgccggag cgtcgttgag aagcgcctgc acttccggat tttttgaatc cagcacgcgc
    25321 agcgggttag tgtacatgcg gcgtttgcag tcttcgtcca gcttttcttt atgctgctca
    25381 aggaatgcca ccagcgcatc gcggtaattg gcgcgtgctt ccagcgaacc aatagagttc
    25441 agctcaagag ttacgtgttc gaagataccc agcgcacgcc accagcgggc agtgagcata
    25501 atcagttcag cgtcgatatc cggaccttgc agaccgaaaa cttcgcagcc caactgatgg
    25561 aactgacgat aacgcccttt ctgcggacgc tcgtgacgga acatcggccc gatataccac
    25621 agacgctgtt cctgattgta cagaagacca tgctcgatgc cggcgcgtac acagcccgcc
    25681 gtcccttcag ggcgcagagt caggctgtcg ccattgcgat cctcaaaggt gtacatctct
    25741 ttttcaacca cgtcggtgac ttcaccaatc gcacgtttga atagcggggt ctgctctaca
    25801 atcggcaagc ggatttcact gtaaccgtag ctgccgagca cgtttttcag tgtgccttca
    25861 atgcgctgcc agatggccgt ttcgccaggc aggtaatcgt tcatgccgcg aatggcttga
    25921 atgttttttg ccacgtttat tctctttctg aatataaaaa tgaaccctca acgcttccct
    25981 caatgtttcg ggagccatgc gggttcaatc atacacggga agcgaggcgc ttcccatcac
    26041 gttattattt ttcaacctgc tgaacgtcaa ttcgacgcgc ttcgtccagc tgactggctt
    26101 tcgcacgaat gcgtgcttcc agctggtcga tcatatcgtt gttgtccaga cggtctttgc
    26161 gcacgccatc ttcatagagg ccgcttttct tgttgccgcc ggtgacgccg agtgtagaaa
    26221 ccagcgcctc acctgggcca ttcaccacgc agccgataat cgaaacgtcc atcggagtga
    26281 tgatatcttc caggcgttgc tccagcgcgt taaccgtacc gataacatca aattcctgac
    26341 gcgaacaggt cgggcaggcg atgaagttaa tccctcgcga acggatacgc agcgatttca
    26401 aaatatcgaa accgactttg atctcttcga ccggatcggc cgccagcgat acgcgcagcg
    26461 tgtcgccgat gccttccgac agcagcagac ctaaaccaat ggcggatttt actgccccgc
    26521 tgcgtgcacc accggcttcg gtgatcccca gatgcagcgg ctgatcgatc tgttttgcca
    26581 gcaaacgata agactcaaca gcgaggaaga cgtcagacgc tttcacgctg actttgaact
    26641 gatcgaagtt caggcgatcg agatgatcaa catgacgcat ggcggattcc agcaacgcct
    26701 gcggcgtcgg ttcgccatac ttttcttgca gatctttttc cagcgatccg gcgttaacgc
    26761 caatacggat cggaatgttt ttatcgcgcg cacagtcaac caccatgcga atacgctctt
    26821 cattaccgat attgccaggg ttaatacgca gacaatcgac gccgtattcc gctactttca
    26881 gcgcaatgcg atagtcgaag tggatgtcag ccaccagcgg cacgttaacc cgctgtttga
    26941 tgagtttgaa cgcttctgcc gcgtccatcg tcggtacgga gacacggacg atatcagcgc
    27001 caacgcgttc cagcgccttg atttgattga ccgttgcttc gacatctgtc gtacgcgtat
    27061 tggtcatgga ctgtacggcg atgggagcac catcgccaat cggcacattc ccaacgtaaa
    27121 tacgtgttga ttttctacgt tgaattggag cctggttatg catgaaaaat ctcccgcgtt
    27181 acccgtctgt tactgcgccg gtgattgttc ggcattgagg gtcagacgcg caacctggtt
    27241 agttctgata aaacgactca gatcgacagg tttcccttga tactggatct gtactgcggc
    27301 tggcgcacca attttcagtt tatacggtgc ctggccggtt aaattcaaat taccgtcttt
    27361 acgctgcatc ccgctaaaca attttttacc ggtagcatca gtgacctcca gccagcaatc
    27421 ggcagtaaag ttcatcacca gcgcattcgg atcagccgcc ggcgtggtca cgccagcctg
    27481 atcggttggc aacggcgcag caccatctgg cgttgttgct gccgttggta ccggggtcgc
    27541 ggcggtatca acatttgcct gcgaaggcga aacaaccgca ttctgttgcg gatcaacagc
    27601 tggtgctggc gcagttacgg caggtgtttg cgtgttggtt gcggtagtat ccacagacgc
    27661 tggcggcgtg gacgttgtag ccgggtctgt agttgtcgac gtatttaacg ggacactctg
    27721 cccctgctcg ctattactgc tcagttctgc cgaagattga tcggccatag tggtgatctc
    27781 ttcctgctga gctttgtggt cttgccacca ccaggcaccg ctcaggccga taaccacaaa
    27841 caacaccagc caggtgaagg tcatcagcca gccatcacgt tttttgcggc gtttaccgag
    27901 ggaaaaactc tgcattggcg caacttttgc agcccgtagt ggagcctgct tttccagccc
    27961 tggcagcagt tcttcttctg gaatatgtac cagacgcgca taagagcgga tatatccgcg
    28021 caggaatgtt gaagcaagat cggcgggtgc cttatcttct tcaatgtcgc gtaccgtgga
    28081 aaccttcagg caaagtcgct cggcaacggc ctgctgacta agtcctagtt gttcgcgagc
    28141 attacgcagg cgagcgccgg tagtaagtgc ttcattttgg tcgtgcgtgg cttcagtatt
    28201 cattcgctgc aggtacgttt aaatgagaat tgagatgccg gtgaaccatc atgctcaccc
    28261 acaccgcgaa acatccgtta agttaaccgt tatcatacag tataagaccg tctgtgtgct
    28321 cgtgacaaag cccacattca ctaaggctaa cttactgttg catcgttaca tactgcctta
    28381 aagtcagcaa aaacgcaccg ttaatattga ccagacaatt gcaacttaat acttcattca
    28441 taaaacatta cgccacggta cataaagtaa ccgtggcgta atggctatca gaccgcttta
    28501 atgtcgatgg cttcaccctg catccgttta cgcagggtac gtttcgtacg gtcgataaca
    28561 tcgcccgcca actgaccaca ggcagcatca atatcatcac cacgagtttt acgcacaata
    28621 gtggtgaaac cgtagctcat cagcactttt gagaaacggt cgatacggct gttcgagctg
    28681 cgtccatacg gcgcacccgg gaacgggttc cacgggatca ggttgatctt acacggcgta
    28741 tctttcagca gttccgccag ttggtgcgcg tgttcagtgc cgtcgttaac gtggtcaagc
    28801 atcacgtatt caatagtgac tcggccctga ttggcgttgg atttctccag ataacggcgc
    28861 accgcagcaa ggaacgtttc gatattgtac tttttgttga tcggcacaat ttcgtcacga
    28921 atttcgtcgt tcggcgcgtg cagggaaatt gccagtgcaa cgtcgatcat atcgcccagt
    28981 ttatccagcg ccggaactac accggaagta gaaagcgtga cgcgacgttt agacaggcca
    29041 aaaccgaaat catcaagcat gatttccatc gccggaacga cgttgttcag gttgagcagc
    29101 ggctcgccca tgcccatcat cactacgtta gtgattggcc gctgaccggt gacttttgct
    29161 gcgccgacga ttttcgccgc ccgccacacc tggccgataa tttccgacac ccgcaggttg
    29221 cggttaaagc cctgctgggc ggtggaacag aatttacact ccagcgcaca ccccacctgc
    29281 gaagagacgc agagcgtggc acggtcgtct tccgggatat acaccgtttc gacgcgctga
    29341 tcgccaacgg cgatcgccca tttaatggtg ccgtcggatg aacgctgttc ttcaaccact
    29401 tccggtgcgc ggatctccgc cacctctttc agtttgccgc gcaacacttt gttgatgtcg
    29461 gtcatctcat caaagttgtc gcagcaatag tgatacatcc acttcatcac ctgatcggcg
    29521 cggaagggtt tttcacctaa atctttaaaa aactcccgca tctgctgacg gttgagatcc
    29581 agcaggttga tttttccatc tttcgtggtg acgttttcag gtgtgactaa ttgttcagac
    29641 atatgctatt ccggcctcgt tattacacgt tatggcccct ggagggttga aaaaagaaac
    29701 gccccggtga gcggctgctc gtccgggggc gctgcattgt acaaattctg gcgcacggat
    29761 gccacgtttg cacgcggcat ttacgaaatt attaacgggt gcgcgggcac acttcgcctt
    29821 cgccgaagaa ataagcgatt tcgcgagcgg cagattcgac ggaatcagaa ccgtgggtac
    29881 cgttttcggt caggctgtca gcgtaatcag cgcgcagagt accggccagt gcgtttgccg
    29941 gattggtcgc gcccagcaga tcgcggtgac gctgaacggc gttttcacct tccagcacgg
    30001 aaaccacgat cgggccagag gtcatgaatt caaccagacc atcaaagaac ggttttccat
    30061 cgtgttcagc ataaaagcca cgagcctgtt caacggtcag gtgcagcatt ttggtgccga
    30121 caattttgaa ccctgcagct tcaaagcgcg caaagatatt accaatgacg ttttttgcta
    30181 ccgcgttcgg tttgatgatg gaaaaagtac gttcaatagc catttttacc tctgtaaatt
    30241 gttctgttgt tgtctgtacc agcgtacgag atggcgcgga ttataatgag caacagggcc
    30301 gttgactatt gatgaaggta acattttttt aaaataaaat gagttttagc aacaatcagg
    30361 gacaaaacag actattgcat gacaaatttc actgtcgcga tttgccccac gtcatccatc
    30421 accagcaatt gataatcacc tttatccgtc aaatgcaggg tcacgttgcg cccgcgttca
    30481 gttaacggtt cgccattcag aaaccaccag cgttcacctg cccctccact tgattgcaac
    30541 ggcaaagtcg cttccgctgc gcccggtaaa cgtttaataa tcgcgccatc gcgaacgcct
    30601 gtcagctgca gagggagctg ggcatcgtga ccgtatggcg ggcaaattgt cgaggccggt
    30661 ggtaagcgca cagcgcgacg ttctgatgcg ggcagccagg gttccagcgg tagcggccag
    30721 acatttacca tttcttgctt cgcctgcggg cagtcagcgg cgacgcgctg ccctttgtca
    30781 tccagccaga tggggaagcg aatgccatta atcccttcct gctctgacag taacagcgtt
    30841 ggcggctggc tgccatccag cagccaggtt gccaggcggc ggcgacagtt accgtcacct
    30901 tccggcaaag actgtccgcc cggccagcag ataacgccac gagtgactga gttcgggcgc
    30961 gggtcttccg gcagattcgc actgcgcgac agtaagatat tattgacctg attcaacaat
    31021 ggtacggcac tggcaaagcc aaactgacca acaacgggcg tgccgtcagg tctgccagtc
    31081 cagatcccaa tgacatagcg agcgttaacc ccaatcgccc aggcgtcacg atagccatag
    31141 ctggtgcccg ttttccatgc cagtggggcg acgcgcggca aggcactatc cggcaagggt
    31201 tgcgcttcat cagccataat ccggcgaatg atccacgccg cccccgacga cattaaaggc
    31261 cgttcaagca gcggatcgtc aggctgtaag cgcaatttgc ctgccttgcc gtggcgagca
    31321 aacgcggtat acgctgccgc catatcttcc agttttgcac cagcgccgcc gagaatgagt
    31381 gaaagattcg gcgcagcgcc gttgggcaaa tataacggca atccaacatt gcgtaacttt
    31441 gccgcaaacc gtttcggtcc ataggcttcc agcacctgca cagcaggtaa gttcagcgag
    31501 cgcaccagcg cctcgctcat gctgatcggg ccatgaaaac cgctatcaaa gttacctggt
    31561 cgataatcac cggtgcgccg ggggacgtct tgcagcagtg atgccgggtg gatcaagcct
    31621 tcatccagcg ccagaccata aacaaacggt ttgagcaccg atcctggcga tcggatcgca
    31681 ttgaccatat caacatgacc aaagcgtgaa tcatcgttga gatcaaccga tcccacccag
    31741 ccgcgaacac gcatatcggt atgatcaacc acgatcatcg ccagtgagct gcgcggtggc
    31801 aaccgccctt tccagttttg cgccagttct tccagacgtc gttgaagacc ggcatccagc
    31861 gtagtaacga ttttgtcgct tttgctttta ccgagcatca tgcgcgaaaa cagcggtgcc
    31921 agttgcggca tttgtcgggg tgccagccag atgggttctt cccttgactc ttttacctgc
    31981 tcacgggacc acacaccttg cgcagccatc cgttcgagca ctttattacg cgcggcttcg
    32041 gcacgctccg gccaacgatc cgggcgaaga cggctgggcg cttgcggcaa aaccgccagc
    32101 attgccgcct cggaatagct taaattcgca ggcgactttc cgagataagc ccaacttgcc
    32161 gcaccgatcc cctgcaacgt accgccaaac ggagcgcggt taagatacaa ggtcagaatt
    32221 tcacgcttag acagatgcca ttccagttgc aacgcgcgcc agagctggcg aattttgccg
    32281 ccaaatgttt tggggtgagg atcaagcaga cgagcaacct gcatagtgag cgtgctgcca
    32341 ccggaaataa cccgtcccga agtgagatct tgccatgctg cgcgcgccac cgagaatgga
    32401 ttcacccccg gatgcttcca gaaccagcga tcttcataat tgatcagcgc ttcaaggtaa
    32461 cgtggagaaa catcttcgat tgttaccgga taacgccaga tgccgtcagc atcggcgaag
    32521 cgccagagcg gcgtaccatc ctgcgccacg accactcgtg cgggattgac ttcatgcagc
    32581 ggtagaggcc agagtttatc tgctccccac gctgcaagaa aaaggagaaa tggcgcagct
    32641 gccagcgtta tccagcagcc gcgtttggtt aacaagcgag gcatttacgg tcggacaatc
    32701 agcagatctt cagccgcgcc ggtcgcccgc cattggggaa catacattga ttccaccatc
    32761 ggttgcggta cctgatacgt tcccggcgtc accgcccgcg ccagatacac cagcgttacc
    32821 ggttggtatt catcaacggc aaccgccgcc acaaagcgat cgtcacggaa ctcaatgtgc
    32881 ttaatactcg cctgctgcat ctggttcagt aagttctgca cttcgccacc actttgctcc
    32941 aggctggcgc taccgttcgc cagattctgg ttttccagtt ccagacccac aggcagcaga
    33001 tccacgacta acgcatccgg cacgctgtta ctggctttta cctgcaacca caccagcacc
    33061 agatcgccgc tacgtaacga gtccagcgat ttgctcttac catcagtacc aagaatatga
    33121 cgctcgattt gcagcacatt gttcgccggt aaaggtgcgg attgcggata accgctggca
    33181 tccatacgca accataacgg ctgatcgcca ctgttgctca cctgcaaggt gacaagttga
    33241 tcgctattca gattgctgtt ttgcgctttc tcgcctgtca gcggctcagc tgagaaagag
    33301 gtttgcgcct gccatttacc cggtaaatcc tgaatcgtac gggcagccag gaacaacgcg
    33361 ttactttcct gcgtcgatag ccagcgttca ccaaacgcct gctgcgaaag tgtgttcagc
    33421 aaagtgtact gctcatcggg tagcagttta ttttcttcca gcaaggagag cattaacgcg
    33481 ttgtcgcgca gtggactacc gtaatcaccc agccatatcc gctcatcact atttctcggc
    33541 gttttcagcg ccagcgcaat cgcttcttca ccacgcgtcg catcacccat ggttttcagc
    33601 gcaatgccaa gttgcagcag cggtaaacca gaagcggcat ctgcacgatg ctcccagatt
    33661 tcacgcagcg cacccagtgg tgctttttgc tgacgggcca gcaccagcgc agcgtaagac
    33721 tgaacggcga atttactggc tttgagatta tccgcgtacg ggatcgacat catgcccgga
    33781 tcttgtaaat agcgcagcag acgctcatta ccccggttaa tggcgtctgt cggcacgctg
    33841 taaccctgtt cgcctgcgcg gaccaggaaa tccatcacgt aagccgtcag ccagtactct
    33901 tcgtcaccgt ttttatccca cagcgcaaag ccgccgttat cacgttgcat ttgcagcaaa
    33961 cgggaaatgc cgatatcgac cgatgcacgg cgtttctcat cactgtcgcc tttgatgccc
    34021 aacgcctgca gttgggctgc gttggtataa agtgacggga acaggccgct ggcggtttgc
    34081 tcaagacagc cgtacggata cgcttttaac tctttgatat aacgtgcaat gttcagcggt
    34141 ggtttgccgc tcaacaacaa ttgcccttcc agcgtaacag gcgagaagtt ttgcaatcca
    34201 tccgccggaa tcgcccatgt ctcaccaggc tgtaacgccg taccgtaatt aaccgtttgt
    34261 gccgggaacg ccggacggac gccgattttc cactgcttat gctgatcggc aacggtttca
    34321 cccggtaacg ctaacccgct aatggtggcc tgaatttctc catcgccata acccggcaat
    34381 gctcgcaccg ggataaacag cgtagtacgc acacctggcg ctaattcaac ggctgcgggt
    34441 gaatcgctga ccagttcaag caaaccactg gcggtcaggg caacgttcag tttttgcggt
    34501 ttatcggtaa gattagtgat atccagcgtc agacgcgagg tatcgccact cgccataaag
    34561 cgcggcatgt tcagttcagc aatcaccggt gcggcaacta tcactttgct ttcgttgcta
    34621 ccgaaatcat ctgccgtcca ggcttgcgcc atgacgcgca attcaccgtt aaaatcgcca
    34681 atcggcagtg taaccgagcc ttcgccctgt tcgttgagcg ttaccgacag cgcctgctgc
    34741 gcgacaatat tgacgtgatt gaccggcggt ttaccaccac gtttcagctc atcaccatcg
    34801 ccaccgaaac gcagagctgc cagacgcccc tgaccttcaa taacctgacc gtaaatatcg
    34861 taaatgtctg cgccatagcg tttctgacca aagaacgcct gccacggatc tggcgtaacg
    34921 tagtcagtaa tattcagcac accactatca acggcagaca ccagcacatt cacctgttta
    34981 ggcttctcgc catttttagt gctggcttta attttcacgg ttaatggttg attgggacgc
    35041 atttttgctg gtgtttccag cgccagatcg aggcgacggt tttcatcacc aagcggcaga
    35101 tgcaacacac caaccgcgcg ttttggcgtc gcggagcgag atttatcgcc aggacgcacc
    35161 accagcgtac tcaaataaag atcatgacga ttccaagttt tatcgaccgg aatcgtcaga
    35221 tccagccctt gagccggaac atcaatctct tgccaccaca gcggcccttc actggactcg
    35281 accatcgcat aacctttacc cgccgttggc gcggcaatat gcaacttaat ggtatcgccg
    35341 gggcgataac tggctttatc tagtttcagc gtgacacggt cgggtcgcac tgcgccgctc
    35401 ccgtcgctgt tgtcctgcca gctatagcca gcccaaaaac gcacgctact gaccgcttca
    35461 ttcggcgctt tgacttccag acgataagcg ccccactcta ccgggaaact gaccttaccg
    35521 gtttcatccg cctgaagatc cagagtttgt tcattttcga tcagatcttt ttgatcaaac
    35581 tgtgactgcc agccttcatc ttctgaccag ttccagtagt aatcgcggcg ttcgcgaatc
    35641 aggcgcacct gcaagcccga cacggctttt ttcacgcctt gcgcatcgct ataaacgatg
    35701 tcaaaagcgg cgttactgcc ttcatcaaca atgggctgtt tcaccgtgct gtcagtgcga
    35761 taatcgtaaa ccgatttcaa ggcgaactgc ggacggagcc ccggcaatgc atcggcaggc
    35821 cagatagcct gctcagcgcg gcgcgtcacc gggcgaccgc ccgattccag caaactgccc
    35881 tggaaaataa cctgcaatgg ggaatgcgtt tccttccact ggctttctgt agaaacttcg
    35941 ccgcgccctt tatcatccag cgtcaactga acttcatcca gcgtgcggga aaggttttcg
    36001 gcagctatat cgccgaattc aaaaccaggt aaggctgaca cagcctcacg cagtgggcgc
    36061 aggaaaagtt gcccttgcaa agtattacca ttagcaggcg caccgtacag gtagtatccc
    36121 accacggaga atttcacttc atcattcggc gtaagcgggg ttttctcacc ggtcagattc
    36181 agcgccatac gctctggcat aaaatcttcg acgtggaaat cccacatccg gtactggtta
    36241 tcgcccgtgt tggcgcgaat atgccacata ccggttgccg cattgctatc gagtggccag
    36301 gtaaagtggt agaggccatt ctccggctga ctaacgacgt tcctgagtac ctgcccgtca
    36361 ggtttaatca cgtctaactt gatgggttga tcgggcaatg ctttaccgtc tgcatcgcgc
    36421 agtaaaccat tgaggattac cgtttcacct gggcgataaa gatcgcgtgg gccaaacatg
    36481 aaaaactgtt tgctatagcc tggcgcgcca gcaatgttaa attctgctaa gtccagcgcc
    36541 ggaagtttta aatcgagtag cgttgtctga ccgtctttac gtgccagcag taatgccgca
    36601 tttttatcat tttccagctg cacatgcccc tgagcgtcac tggttgcctg agtcagagtc
    36661 tgtcctttct catttaataa agagacttca attccttgct gggccgcgcc gttttccaga
    36721 ctttgggtaa agatatccag acgattgtga taacggtgag ctgaaacgcc gatatcactt
    36781 aacgtaaaca gcgtcgcggg attactgtaa tcgtaacgtc cagcctgatt catcacagcc
    36841 agatacacgc ccgcctgctg aagcggtttg atatcgccca gcggcagcaa taatttttca
    36901 cgggtgttac gcgcaggatt gagatcaaac cgtccggtgt agaccagatc cgccatctgc
    36961 agcagtttgt ctgactgcca gttcgccagc gaattgcggt attcccattg gctaatgaat
    37021 gctggcagag attctggctt aacgcggaag aagttaacat cgacattatt aacgttgagc
    37081 gccattaccg gcagcccttc aacgactttg ccaggcagca gcgaaccacg gctggcaaaa
    37141 ccgacgctgg gttggatgtc gcgggtagtt atagtttttt cgtaatcttt actgaaggtt
    37201 gcgttgttga gtgctttgac ctccttgcca atagtaacga tcaaatcacg tttcggttcg
    37261 aggtggcgta aacgtagctc tttaagatta tctgacagct cccaggcacc atccactttg
    37321 ccgctttttt tatcgacgac atgaataacg cgtgagaaat cctgatccgg gtcgagaggg
    37381 atggagaacg tcagcaccag cgtggctgca ccatcaagtt ggacttcaga gagatccagc
    37441 aatgtcagca ccttaccggc actctgttgg gccagttttt gtctctccgg cacggagagt
    37501 tttgctgaac tcgcgttttc tgaagaggcc gctttagtaa cttcagaagg cgcatctttt
    37561 ttcaccgctg ttggcgcgtt atcgttgttg tcgcaccctg ccagcgccag cattagcatg
    37621 caggcggcta cgcgtaactt tttcattttt catccctgcc acaatggccc gttagcaacg
    37681 tcgaataatt attatgcgtg agaatttacg ttatgtaatt cagtatcacg cccatacaat
    37741 ccgaatttta gtgagaattg tggctcagac agccaaatca gcctctttcg cttgtcgctt
    37801 ttcactaaac atccgacaat ttagcctaac cccggcaaaa atggagatgc ctatgtccac
    37861 gacatggttt gtaggagccg actggctcgc cgaacatatt gatgacccgg aaattcagat
    37921 tatcgatgcc cgcatggcgt cgcctggaca ggaagatcgt aacgttgctc aggagtatct
    37981 gaatggacat attcccggcg cagtgttttt tgatatcgaa gcgctttctg atcacacttc
    38041 cccgcttccg cacatgctgc cgcgcccgga aacgttcgcc gtggcgatgc gtgaattagg
    38101 cgttaaccag gataagcacc tgattgtcta tgacgaaggt aatcttttct cagccccacg
    38161 agcatggtgg atgctgcgca cctttggtgt agagaaagtg tcgattctgg ggggtggact
    38221 tgcaggctgg cagcgcgatg atctgctgtt agaagaaggt gcagtagagc tgccggaagg
    38281 agagtttaac gccgcgttta atcctgaagc cgtggtgaaa gtaaccgatg tattattggc
    38341 aagccatgaa aatacggcgc aaattattga tgcccgcccg gctgcacgtt ttaacgcaga
    38401 agttgatgaa cctcgcccag gtttacgtcg cggacatatt cccggagcac tgaatgttcc
    38461 gtggacggaa ctggtgcgcg aaggcgaact aaaaacgacc gatgaactgg atgcgatatt
    38521 ttttggtcgc ggcgtcagct acgacaaacc aattatcgtc agctgcggct ctggtgtaac
    38581 ggcagccgtg gttttgttag cactcgcgac gctggatgtg ccaaacgtga aactgtacga
    38641 cggcgcatgg agtgaatggg gcgcgcgggc agatttaccg gttgagccag tgaaataagt
    38701 attttacagg caataaaaaa ccgccgaatt tggcggtttt ttattgctgg tctggttcgc
    38761 ggcctttcca gcaggttgta ttaccgtagt aatgcaagcg cgtctcagcg gagacaatac
    38821 tcgccagtaa ctctcttttt gtcaagcaaa agagagttat tattgttctg ttagtgtatt
    38881 atccactgcg gccctttccg ccgtctcgca agcgggcgct ggcttaagga aaggatgttc
    38941 cgcgaccgtt agagcaagcg cgtctcagcg cttgctatcg cggcaatatc gccagtggtg
    39001 ctgtcgtgat gcggtcttcg catggaccgc acaatgaaga tacggtgctt ctgtatcgta
    39061 cttattgttt ctggtgcgct gttttcccaa gtaaataata accggagtct ctccggcgac
    39121 aatttactgg tggttaacaa ccttcagagc agcaagtaag cccgaatgcc gccctttagg
    39181 gcggcatatt ttagattatc cggttttgtt taaagtcacg caaaaaacca ccccagcgac
    39241 gttcatagaa tggcacaata tgttcggtaa taaagtggct aattcctttt tccccttttt
    39301 tcacctgaca aatatcgatt ggttcatcgc caggtaatgt atcggtcgct acacttcccg
    39361 tcgcctgaat aatttcttcg atatcaccat cggcttcaat gccaataagt aaattaggct
    39421 gtgcctcttc gttctcttta attgaacaaa taaaagcacg cttcaccggc ttaatggttt
    39481 taaataaggt ggtgagtgaa tcaatcattt gtgctggcgg ctctgcgact tccgataata
    39541 tcagcgattc accgccttcc aggatttcct ggctgctcag cggatttccc tcttcaccaa
    39601 tcaacaaact gatttcacgc ggcataaatt ctttaccggt cggcagtttg gcattaagga
    39661 agagcgtttc gccaagggtc atctcaaaca atgtgcgaac gggcattacg acaaatgcct
    39721 gttcgtcttc aaccgcctgt tgaagcgctt ctaacgaggt gaaaaaagga atgacgctgg
    39781 tgccgtcttc tttttcccag tgctgtaaat caagcgcact atcttcaacc acagcctcgc
    39841 cctgcgccgc cgtaccaggc acccagacgg tggattccag tagagtacgg aaaaaggccg
    39901 ggcggtgcgc cggttcggtt gctgcttttt ccagcaggtc ttcaagttcg ttttttgttt
    39961 cggacataag aaccacaatt cattcaacgt tcggggcgca aatgcctgat gcgctacgct
    40021 tatcaggcct acaagggatt cgcaatttgt tgaatttgca gaatttgtag gccggataag
    40081 gcgtttacgc cgcatccggc atattagttt acgccgttaa cagattagct atcgtgcgca
    40141 caccaagtcc cgtagcgccc gcagaccact gttcaaccgg cgctttacgg taagtcgccg
    40201 agcagtcgat atgcagccag ccttgctgat agttctcaac aaagtgcgac aggaagcccg
    40261 ccgccgtgct cgcgcctgcc ggatacgccg cgcttccggt attgttcagt tcggcaaagt
    40321 tagacggcag ctggctgcgg tggaattccg ccagcggcag acgccagaat ggttcgttct
    40381 cttgtgaggc actcgccagc aaacgaccgg caagcgcatc gtcaaaactg aacagcgcgt
    40441 gataatcatt acccagcgca gttttcgccg ccccggtgag ggtcgccgca tcaatgatca
    40501 tttccggttt ctgcgcactg gcatcaatca gaccatcggc aagcaccaga cgcccttcgg
    40561 catcagtgtt catcacttca acttttttac cgttgcgata ggtgatgata tcgcccagct
    40621 tgaacgcatt gccgctaatc aggttatccg cacagcagag gaacagcttc acgcgcttgt
    40681 tcagtccgcg cgtaatggca aatgccagcg ccccggtaac cgttgccgcg ccgcccatgt
    40741 ccgacttcat cgagtccata aacgcagtct gtttgatgct gtagccgccg gagtcaaaag
    40801 tgataccttt acctaccagg cacgcgtaca ctggcgcttc tttatcgcca gttgggttgt
    40861 aatccagcgc cagcaatacc ggagaacgtt ctgaaccgcg tccgacggtg tgcagcccca
    40921 tataaccttg ctcacgcaga tcttcgcctt tggtgatccg ataagtcaca cgatcgcccg
    40981 cgacgttgct gatcagatca acagcacgct gtgccagttg cgatggtccc aattcttctg
    41041 ccggtgcgtt gatggtgtca cgcacccagt cgatgatcat caggcggtta tccagttcct
    41101 ggcgctgggc atcgtccaga tccggccact ccactttacg cgtgcctttc ggggctttgt
    41161 aaccttgcca gaatgcccag cagcgatccg catcccagcc ttcaccgctt aactgaacat
    41221 gcttgatgcc cagaccgtca atcttgcgcg ccgcacgctg gatcagcccc agatcgtctg
    41281 ccccgttcaa atgcagggta atgccgtcat tattaatgct gtaagttgct ttttctcccc
    41341 agcgcgcgtc ggcaggttgg gtagagaggg taatcttcat cgcttctgtc attttagtta
    41401 tccttctttg taaaagggcc gccagttggc agccccagga tttttccgct aaatgattcg
    41461 cgttgcagaa aggcggcaag tgagtgaagc cccaggagca tagataacta tgtgactggg
    41521 gtgaacgagc gcagccaacg catctgcggc gtgaagcatg acgcggaaat tattcggcct
    41581 cgtccagcca gactaacaaa atcgcttcga ggattttctc gttggatgcc tgcgggtcgt
    41641 cgtcgaaatc ttccagatcg caaatccact gatgcatatc ggtgaatcga accgttttcg
    41701 gatcaagatc aggatacgca tcgtacagtg cttcgccaat ttcgcggcta tcggtccact
    41761 taagtcccat actaacctct gttaatgctc acgcgcatgg ttgatagtgt aacgcgggat
    41821 ttcgactact aaatcttcgt cggtaacgcg tgcctggcag cttaaacggc tttcgggctc
    41881 cagtccccag gctttgtcca gcatgtcgtc ttcctgctct gagctttctg gcagcgagtc
    41941 aaaaccttca cgaacgatgc agtggcaggt ggtgcaagca caggattttt cacaggcgtg
    42001 ttcaatctcg ataccgttac gcagagctgc gtcgagaatg gtttcaccgc tattagcttc
    42061 cagaacagcg ccatcaggac agagatcctg atgaggcaaa ataacaatct ttggcatatt
    42121 aaacctcgtc cacggaatgg cctttcagcg cacgacgaac cgactggtcc atgcggcgag
    42181 cggcgaaatc ctgggtttgt ttgtctacgt ttttaatcgc ttgttcgatg gcgtcaacgt
    42241 catcgccctg cgccacttca ctcaggtgag cggcagcatc gtcaatgacc tgacgttctg
    42301 cggcgcttaa cagcgcggca tcagcagcca gcgcgccgtg cagactttcc agcacacgcg
    42361 ccgcttctac tttttgttct gccagcattc gggcttttac gtcctgctcg gcatagctca
    42421 ttgagtcttt gatcatcgaa gcgatttcgc tatcggtcag accgtaagac ggtttgacct
    42481 gaatagacgc ctcaacgccg gtggatttct ccatcgccgt cacgctcaaa agaccgtcgg
    42541 catcgacctg gaacgtcacg cgaatatgcg caccaccagc cggtagcgcc ggaataccac
    42601 gcagcgcaaa acgcgccagt gagcggcagt cctgcaccag ttcgcgctca ccctgcatta
    42661 catggataga catcgccgtc tgaccatctt taaaggtggt gaaatcctga gcgcgggcca
    42721 ccggaatagt ggtattacgc ggaatcactt tctccaccag gccgcccatc gtttcgaggc
    42781 ccagcgacag tgggatcaca tcaagcagca gcatttcgct gtctggcttg ttacccacca
    42841 gaatatccgc ctgaatcgcc gcgccaatag cgacgacttt atccgggtcg atggaagtca
    42901 gcggtggacg accgaaaaat tcgcctaccc gttcacgcac cagcggcacg cgagtagaac
    42961 cgcccaccat caccacttcc agcacttcgt cagcttctac acccgcgtct ttaagcgcgc
    43021 gacgacaagc cagtaaggtt cgttttacca gtggcgcgat cagttcattg aattgttcac
    43081 ggctgatttc gccctgccag cccgcaacgt taacggtcac ggagtccgca tcgctcagcg
    43141 cgattttggc tgcaatggcg gcatccagca gttcacgctg aacgcggtta tcgctacgat
    43201 caggaatgcc cgcctgctcg cgaatgtaat ccgccagcag atggtcgaaa tcatcgccgc
    43261 cgagcgcgga atcaccgccg gttgccagca cttcaaacac gccgcgactt aagcgcagaa
    43321 tggaaatatc aaacgtcccg ccaccgaggt cataaacagc gatcacgcct tcctgaccgg
    43381 aatccagccc gtaggcgata gccgcagcgg tcggttcgtt aagtaagcgc agaacatgca
    43441 accccgccag acgcgccgcg tctttggtgc cctgacgctg ggcatcgtca aagtacgccg
    43501 gaacggtgat aactacacca tccagctcgc ctgccagggc ttcagttgcc cgcgccgcca
    43561 gtgctttgag gatgtccgca gaaacgcgca ccgggttcag cagccccgcc gccgtttcaa
    43621 tcatcggcag gccgttttcg ctggcctgga attgataagg cagatgcgga tagcgttgct
    43681 ggatatcagc cagcgagcgc cccatcaggc gtttaacaga actaatggtg ttggcggtgt
    43741 caagcgctgc gttagtacgc gcgtcataac ccaccgaatg cccttgctgt tgatagtgaa
    43801 caacagatgg cagcaggtga cggccttcat ggtcggctaa cgtttcggcc tgaccgctgc
    43861 gcactgtcgc caccagcgag ttggttgtgc ccaggtcaat accagccgcc agacgacgct
    43921 gatgcggcgc agcactcaaa ccaggttcac taatttgtaa taaggccatg tttagcttcc
    43981 agaaattaaa aatcgagcag tttttcttcg agttgttcgg cactgctccg cagtttatcg
    44041 agaaaacgca gcttacgcac ggtatccgcc gccgcgtccc acgcctcgtt gtctaactgt
    44101 tcaaccatca actgatggcg ggtatcaaac atctttttca cacgtttgat aaagctttcc
    44161 agccgcgctt catctttcgc ctgttcgatc tcgtccagct cttcgcgcag ctccaactgt
    44221 tccatcagga acgcggtgtc gcgcacagta tgctgctcgc tggcgagatc aaaaccgtgt
    44281 aaagaaagca aatattccgc gcgcattaac ggatgacgca gcgtttgcca ggcctggtta
    44341 atggttgcag attgctgtac ggcggcgagt tgttccgcct ggcttccgct ggcgaattta
    44401 tcaggatgat actgacgttg tagatcctga aaacgcaggc tcagcgcctg ggtatcgagt
    44461 tgataacggg caggcaagcc aaagaaggtg aagtaatcca taacattctc agggctgcga
    44521 tccgccgcga taacggcgaa tcaggtaaaa caaaccccac gcgcaggcga ccacggtggg
    44581 gttatcggta tgcgcatcaa acgtggaagc tttcgccgca accacactca tctttgacgt
    44641 tcgggttggt gaatttaaac ccttcgttca ggccttcttt tacgaagtcc agctgcgtac
    44701 cgtccagaaa ttgcaggctt ttgccatcga ccacgacttt cacgcctttg tcttcaaaca
    44761 cgatgtcttc cggcgtcggt tcgtcaacaa attccagtac ataagccata cctgaacatc
    44821 cggaggttct cacgcccaga cgcaggccaa accctttgcc gcggttagcc agaaaggtat
    44881 ttactcgcgc tgctgcactg tcgctcagtg taatcgacat aaccaaacct caactcttat
    44941 tttgcttcac gtttgctttt atagtccgca atggcggctt tgatcgcgtc ttctgccaga
    45001 atagaacagt gaattttcac cggcggcagt tcaagttctt cagcaatatc ggtatttttg
    45061 atcgcctgcg cttcgtcgag agacttccct ttcacccatt cggtgaccag ggagctggaa
    45121 gcgatagcag aaccgcagcc gtaagtttta aaacgcgcgt cttcaatgat accttcatcg
    45181 ttgactttaa tctgcaactt catcacgtcg ccacaggccg gtgcccccac catgccgctg
    45241 ccgacgttct cgtcgttgtt gtcaaaggaa cccacgttac gcggattctc gtaatggtcg
    45301 ataacttttt cgctgtaagc cattataaat tctcctgatt ccgataccga ttaatgatga
    45361 gcccattcga tgctgttcag atccacgccc tgcttgtaca tttcccacag cggagaaagg
    45421 tcacgcagac gaccgatgga tttacgaact aactcgatgg tgtagtcgat ctcttcttca
    45481 gtagtaaaac gacctaaaga gaaacggata gagctatgtg ccagctcgtc gttcagcccc
    45541 agcgcgcgca gcacgtagga cggttcgagg cttgctgacg tacaggcgga acctgaagaa
    45601 actgcgaggt ctttcagcgc cataatcagc gactcacctt caacgtagtt gaagctgacg
    45661 ttgagaatgt tcggcgcacc gtgttccagg tcaccgttca ggtaaacttc ttcgatatct
    45721 ttgatgccgt tccacagacg gttacgcagg ccgcgcagac gttccatctc ggtcgccatc
    45781 tcttcttttg cgatgcgata ggcctcgccc attccgacga tctggtgaac aggcagagtg
    45841 ccggaacgca taccgcgctc gtgaccgccg ccgtgcattt gcgcttcgat gcgtacgcgc
    45901 ggtttacgac gtacatacag cgcaccgata cctttcgggc catagatttt gtgaccggag
    45961 aaagacatca ggtcaacttt caactggctc aggtcgatag gcagtttgcc cacgctctgg
    46021 gttgcatcaa cgtgatagat aatgccacga gcacggcaca tttcgccgat agccgcgata
    46081 tcctgcacca cgccgatttc gttatttacg tgcatgatgg aaacaaggat ggtgtcatca
    46141 cgcatcgctg cttcaagttc tttcagatcg ataatgccgt tacgctgcgg tgccaggtag
    46201 gtgacttcaa aaccttcgcg ctccagctga cggcaggtat ccagtaccgc tttgtgttcg
    46261 gttttgctgg tgatgatgtg cttgcctttt ttctgataaa agttggctgc acctttgatc
    46321 gccaggttgt cagattcggt tgcaccagag gtaaagacga tttcacgcgg atcagcgccg
    46381 accagatcgg caatctgatt acgggcgata tctaccgctt cttcagcctg ccagccgaaa
    46441 cggtgagaac gggaggccgg gttaccaaag gttccgtcca tcgtcataaa ctgcatcatt
    46501 ttctcggcaa cacgcgggtc caccggcgtg gttgcggagt agtcgagata aatcggtaat
    46561 ttcattgctc tataaactcc gtacatcact caatgcaagg aaccaggcta ccggctggat
    46621 gtacgaccgt gtttacggag cgcgatacgc cccggcctga ttctaatatc ttgttatctt
    46681 taacctaact gtttcgggtt tcagcaagtt gacaaactta cgaatctccg ggagcttaca
    46741 taagtaagtg accggagtga gtatgtgcag tcaacgcaga tgcaacacga aacaggacgg
    46801 ttaaattaag cgcgtaactt aacgtcgatc gcgtcttgta tgcgggtgcg tggcgcgtcg
    46861 tgagtatgct gacgaccaga cacatccagc acttcctggt tattaaccag ttcgcctaaa
    46921 gtaatgttgt tgagaaaacc ggtgagacgg tcgctcaaat cacgccacag cgcgtgggtc
    46981 aggcatttat cgccgccctg gcaaccgcct ttaccctgac aacgggtagc atctacagat
    47041 tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc tggcatcttt gcctaacaga
    47101 taaccaccgc ctggtccacg tacgctggaa accagaccat ttttacgcag acgggaaaac
    47161 agttgttcca gataagaaag ggaaattccc tgacgttcgg aaatatcagc caacggtacc
    47221 gggcccgctt cagagttgag cgcaacgtca agcattgcgg tcacggcata gcgcccttta
    47281 gatgtcagtc tcatgtctta cttcacctca aactcgcccc tgcccggggt tttttattgt
    47341 aaaagtgggg gtattgcata gcagggtcaa gtctgacatt cccgagtaaa ttggtcaact
    47401 atttacttga ctgatttagt cgggtattta accttcagtg ccattttttt atcgtggcgt
    47461 ttgtacctgt tgtccttcca gaccatgggc gggttgaggg caattattcg cattagcgct
    47521 ggcctcttcc cctcacccta accctctccc cgaaggggcg aggggaccgt ccgtgcattt
    47581 atctggctta gccctcaggc cttattacct ttattctgct gctctataga agccagaatc
    47641 ccgcgcagga tattcaattc ctggctttcc gggcgcgcac gggtaaacag acggcgcaat
    47701 ttattcatca cctgccccgg atggttttca cggataaaac cggttgccag cagcgtttgt
    47761 tccagatgac cataaaaacg ctccagatca tcgaccagcg gatacggcgt ctcttcatgc
    47821 tcgacctgct cgccattttc ctgagttgcc agccaggcca tacgcacttc ataggcaata
    47881 acctgaaccg ccattgccaa gttcagcgaa ctgtattccg ggtttgccgc aatcgcgaca
    47941 tgataatggc atttctgcaa ctcttcattg gtcaagccga cgcgctcacg accaaacacc
    48001 agcgccaccg gggtatttgc cgcttcagcg acgcttttca gaccgcattc gcgcgggtcg
    48061 agcatcggcc acggcagcgt gcgggagcgt gcgctggtgc ccaccaccag gctacaaccg
    48121 gctaacgctt cgtcgagcgt atcgacgatg tgagcattac caatcacatc gctggcccct
    48181 gctgccaggg caatcgcctg ggagtcgggt ttcaccagtg gattaaccag ccacagattg
    48241 gttaatccca ttgttttcat ggcacgggca acagaaccca tattgccggt gtgtgacgtc
    48301 tccaccagca caattcgaat attttgcagc attgtctttc ttcatctaaa gattattcac
    48361 gcatcttatc ataaaacgaa gacagatgcc gatctcgctg ctatactctg cgccgttttc
    48421 ccgttcttta acatccagtg agagagaccg atgcatccga tgctgaacat cgccgtgcgc
    48481 gcagcgcgca aggcgggtaa tttaattgcc aaaaactatg aaaccccgga cgctgtagaa
    48541 gcgagccaga aaggcagtaa cgatttcgtg accaacgtag ataaagctgc cgaagcggtg
    48601 attatcgaca cgattcgtaa atcttactca cagcacacca tcatcaccga agaaagcggt
    48661 gaacttgaag gtactgatca ggatgttcaa tgggttatcg atccactgga tggcactacc
    48721 aactttatca aacgtctgcc gcacttcgcg gtatctatcg ccgttcgtat caaaggccgc
    48781 accgaagttg ctgtggtata cgatcctatg cgtaacgaac tgttcaccgc cactcgcggt
    48841 cagggcgcac agctgaacgg ctaccgtctg cgcggtagca ccgctcgcga tctcgacggt
    48901 accattctgg cgaccggctt cccgttcaaa gcaaaacagt acgccactac ctacatcaac
    48961 atcgtcggca aactgttcaa cgaatgtgca gacttccgtc gtaccggttc tgcggcgctg
    49021 gatctggctt acgtcgctgc gggtcgtgtt gacggtttct ttgaaatcgg tctgcgtccg
    49081 tgggatttcg cggcaggcga gctgctggtt cgtgaagcag gcggcatcgt cagcgacttc
    49141 accggtggtc ataactacat gctgaccggt aacatcgttg ctggtaaccc gcgcgttgtg
    49201 aaagccatgc tggcgaacat gcgtgacgag ttaagcgacg ctctgaagcg ttaatgactc
    49261 aggcgggtga tgtcactcac ccgccctcgc ctttcaggcg ctattctgaa atacttcctc
    49321 accgctttac tttctttcgt cactctccca ccatctttcc ccgattaatg gatgataaag
    49381 aagtcacaag gaaaggttat ggcgctgcca gtgaacaaac gcgttctcaa aattctgttt
    49441 attctctttg ttgttgcctt ctgcgtttat ttagtgccgc gcgttgccat caacttcttc
    49501 tattatcctg acgataaaat ttacggtccc gacccctggt cggcggaatc cgtcgaattt
    49561 acggctaagg acggtactcg tctgcaaggc tggtttattc cttcttcgac gggccctgct
    49621 gacaatgcca tcgcaaccat cattcatgct cacggcaatg ccggaaatat gtccgcccac
    49681 tggccgctgg tcagttggtt acccgagcgt aatttcaacg tttttatgtt tgattatcgc
    49741 gggtttggta aatcaaaagg cacgccgtcc caggccggat tgctggacga tacgcaaagt
    49801 gccatcaatg tggtgcgcca tcgcagtgat gtaaacccac aacggctggt gctgttcggg
    49861 cagagtattg gcggggcgaa tattcttgct gttattggtc aggatgatcg tgaaggcata
    49921 cgtgcggtga tcctcgactc cacatttgcc tcttatgcaa ccatcgccaa tcagatgatc
    49981 cccggcagtg gctacttact tgatgagagt tacagcggcg aaaattatat cgccagcgtc
    50041 agcccgatcc cgcttttact cattcacggt aaagctgatc acgttatccc atggcagcac
    50101 agcgaaaagt tgtatagcct ggcaaaagag acaaaacggt tgatcctaat cccggatggc
    50161 gaacacattg atgctttttc cgatcgtcac ggcgatgttt atcgcgaaca gatggtggac
    50221 tttatcctta gcgcgttgaa tccgcagaac taacccatga tcgctagcac gataatcatt
    50281 cacaaaacca ccttaagaca tgctaatcca ctggtcagaa cagtttaaga tgagaaaaat
    50341 tctgtgacgc ttgccaacat ttctgatgat tagcattccc ttcgccatct ccctgagcaa
    50401 actttagcta ttcttatcaa ttatgcttat gggagatcta cagatgatgc ctacgcttgc
    50461 tccaccatct gtcctttcgg ctccccagcg ccgctgccag atcttgctga cgctctttca
    50521 gccggggttg accgccacca tggcaacctt cagcgagctt aatggtgtgg atgatgatat
    50581 tgccagtctt gatatcagcg aaacaggacg ggagatcctg cgctatcatc aactcacact
    50641 gacgactggt tatgacggta gctaccgggt tgaaggtaca gtgcttaacc aacgtttgtg
    50701 tttatttcac tggctacgac gtggtttccg tctgtgtccg tcatttatta ccagccaatt
    50761 cacccccgcc ctgaagagtg aactgaagcg gcgcggaatt gcgcgtaact tttacgacga
    50821 taccaatcta caagcgttag tgaatctctg ctcccgacgg ctgcaaaaac gctttgaaac
    50881 gcgcgatatt catttcctgt gtctgtatct gcaatattgt ttgctgcaac accacgctgg
    50941 aattacgcct cagtttaatc cgctccaacg tcgctgggcg gaatcctgcc ttgaatttca
    51001 ggtagcgcag gaaattggac gccactggca gcgtcgggcg ctccagcctg taccgcctga
    51061 tgagccactg tttatggcat tacttttttc catgttgcgg gttcccgatc cattgcgtga
    51121 tgcgcatcag cgagacagac aattgcgtca gtctatcaaa cgtctggtaa accattttcg
    51181 tgagctggga aatgttcgtt tttatgatga acaggggtta tgcgatcagc tttataccca
    51241 cctcgcccag gcgttaaatc gcagtttgtt tgccatcggt attgataata ccctgccgga
    51301 agagttcgcc agactgtacc cacgcctggt gcgcaccacc cgcgcggcgc tggccggatt
    51361 tgaaagtgaa tacggcgtcc atctttctga tgaggaaagc ggtctggtcg cggtgatttt
    51421 cggtgcctgg ctaatgcagg aaaacgatct gcatgaaaaa cagattattc tactgaccgg
    51481 gaatgatagc gagcgagaag cgcagattga gcagcagtta cgcgaactaa cgttactgcc
    51541 gctcaacatt aagcatatgt cggtaaaggc atttttgcag acaggcgctc cgcgtggcgc
    51601 ggcactgatt attgcgcctt ataccatgcc attaccgctc ttttcaccac cgctgatcta
    51661 tacggacctg acgctgacaa cacatcaaca ggagcagatc cgcaaaatgc tcgaatcagc
    51721 atgagggaac aactttcggg cgcaaaaaca ttgccggaag cgccaccagc gccattaccc
    51781 agaacacgcc gtggcccaga tattgataca ggaaaccggc gaaaacggtc atgatagcga
    51841 tactgccgcc catcgcgacg gcagagtaaa ccgcctgtaa acggatgact tcgctaccct
    51901 ggcgaacagc aatatagcgc atggcggcca ggtggcagac cgtgaaggtg ccgcaatgca
    51961 aaatttgcac cactatcaac cacggcaacg ccgtagttgc tcccataatg ccccagcgca
    52021 ctacgccgca aatcgccgag atcaacagca tatcgcgagc actacaacgg cggaaaagtt
    52081 tattactcag cgcaaagata atgacttccg ccaccacgcc cagcgaccac aaatacccca
    52141 ccgccgaggc cgagtagcca gctgcctgcc agtaaatggc gctaaaaccg taataggccg
    52201 aatgtgcccc ctgcaataaa caaacgcagg ccagaaagcg ccagttctgg cgaaccagcg
    52261 ccaaccacgc agaccaaccg gtgctctcct gctggcgact tgccccttgt ggctgaatcg
    52321 tcggacggat gagaaagccg agcagcatgg atgccacgcc caacgtcaac agcgcgagga
    52381 tcacccgata atcaaacata gtgaccagtt tgcccgtcag cgccgagcca atgacaaacg
    52441 ccaccgagcc ccacagtcgc actttgccgt aatcaagcgg gaactgcttt tgccacgtat
    52501 tcgccagtgc atcggtcaac ggtaccagcg gtgagaaaaa gaggttaaag ccaatcatca
    52561 ccagcatcag ccacgctacg tgcgcccccg cccagaaggc gacagcaaag agaagtgtca
    52621 gcagtgccag cacgcgcaag gcggaaatca ggcgggaagg atcgctgacg cggggcgcga
    52681 tgagcaaact cccgagaaaa cgggcaacca gacctgcccc caataacagg ccgatggttt
    52741 ctggcgttaa accaatccct ttaagccaga cgctccagaa aggtagaaaa atgccgtaac
    52801 taaaaaagta tgtgaaatag ccgagcgcca accagcgcgt ggattgcaaa accatgagtc
    52861 cctcccgtca ggaggcgtta gtctggggtt aatcgaatgt tggtgcaagt tgaaaaataa
    52921 gaatattcga gcctgcttcc cgatgtctgt aacgccagtt cacaagcact aaaaaactgc
    52981 gatccgcgac gcatgttgca aaaataatgc gtaattatgc cgttacgctt gccaaacgtt
    53041 cctgcacaat ggcgatgaaa tcgcgtaatg ccggcttcat ctcccctttc ttccacgcca
    53101 tcagtaaagc aatggaaggc acatttccgg caatgggacg aaaaacaacc tgcccggtat
    53161 taaaattatt catataaccg ggtatcaaag tgacgcccag ccccatgccc accagattca
    53221 tggtcaccag aatattcgtt gccacctgga cgatatttgg ctggctgttt tcttgcgcaa
    53281 accacgcttt aacgatcggc gcaagcgaac cggaatacgc cggatcggta ctgacgaaat
    53341 tcacgccatc cagttgcgcg gcggtgatct ctttttcatg tgctaaaggg tgatcaaccg
    53401 gtaacaccac cactaatggt tcgtcaaaaa gctccagata atcaatctcc gggctataaa
    53461 caggatggcg cattaagccg acgtcgagtt caccacggcg aattttttcc tcctgttgcg
    53521 tggtgattaa actcaccagc tcaatcaagg tgtctggctg tctgagacga aacattggta
    53581 atacttttgg caataaattc acttccgccg atggcacaaa gccaatggtt aattgtctgt
    53641 cttcctgaac aattttccgc gcccgtaatt tggcattttc cgcttgttcg aggattgcca
    53701 gcgcgtcctg gagaaaacat tctcccgccg ccgtcaacgc gactttgcgc ttatccctca
    53761 ccagtaacgg aacaccgaca cagttttcaa gatcgcggat ctggctgctt aacgaaggct
    53821 gtgaggtatg cagtttttcc gccgcacggg taaagttcag tgcctgcgcc actgcgacga
    53881 aatagcgtaa atgccgtagt tccatcacct tccccttgtt atcgaaaaaa cgtctcagct
    53941 ggtagaaatt aaccatttca catattagca accaaccagc aacatcctta tgacacaaaa
    54001 atagaaggtc aatacatctt atctttcagg attaaaaaat atgaccacac cctcagattt
    54061 aaacatttac caactgattg atacccaaaa tggtcgggtc actccgcgta tttataccga
    54121 cccagatatt taccaactgg agcttgaacg tattttcggc cgttgctggt tatttctcgc
    54181 ccacgaaagc cagatcccaa aacctggtga tttctttaac acctacatgg gggaagatgc
    54241 ggttgtcgta gtgcgtcaga aagacggcag cattaaggcg tttctcaacc aatgccgcca
    54301 ccgggccatg cgtgtgagtt atgcagattg cggcaacact cgcgccttta cctgtccgta
    54361 tcacggctgg tcttatggca ttaacggcga gttgatcgat gtaccgctgg aacctcgcgc
    54421 ctacccacaa gggttgtgta aatcccactg gggactaaac gaagttcctt gtgtggagag
    54481 ttataaaggg ctgatttttg gcaactggga taccagcgca ccgggcctgc gtgattacct
    54541 gggtgacatt gcctggtatc tggatggcat gctggatcgt cgcgaaggcg gcaccgaaat
    54601 tgtcggcggc gtacaaaagt gggtgatcaa ctgtaactgg aaattcccgg cagagcagtt
    54661 cgccagtgac cagtatcatg ctctgttcag ccatgcttct gccgttcagg tattaggggc
    54721 gaaagatgat ggcagcgata agcgcctcgg tgatggacaa accgcccgcc cggtgtggga
    54781 aaccgccaaa gatgcgctgc aatttggtca ggacggtcac ggtagcggtt tcttctttac
    54841 tgaaaaaccg gatgctaatg tctgggtcga tggcgcagtt tcaagctatt accgcgaaac
    54901 ctatgccgaa gcagaacaac gtttaggtga agttcgcgcc ctgcgcctgg cgggtcataa
    54961 caatattttc cccacgcttt catggctcaa cggcactgcc acgctccgcg tctggcatcc
    55021 gcgcggccct gatcaagttg aagtgtgggc gttctgtatt actgacaaag ccgcctccga
    55081 tgaagttaaa gccgcttttg aaaacagcgc cactcgtgct tttggtcctg ctggttttct
    55141 cgagcaggat gactcggaga actggtgtga aatccagaaa ttgcttaaag gccaccgcgc
    55201 ccgcaacagc aaactgtgtc tggaaatggg gcttggtcag gaaaagcgcc gcgacgacgg
    55261 cattcctggc attactaact atatcttttc agaaactgcc gctcgtggaa tgtaccaacg
    55321 ctgggccgat cttctgagta gcgaaagctg gcaggaagtg ctcgataaaa ccgccgctta
    55381 ccagcaggag gtgatgaaat gagtgcgcaa gtttcactag agttacatca ccgcattagc
    55441 cagtttctct ttcacgaagc cagcttactg gacgactgga aatttcgtga ctggctggag
    55501 cagctcgaca aagagattcg ttacaccatg cgcaccacag ttaacgcgca aacacgcgac
    55561 cgccgcaaag gcgtccagcc accgacaacc tggattttta atgacaccaa agaccagctg
    55621 gagcggcgaa tcgcccgtct ggaaacgggc atggcctggg cagaagagcc gccgtcacgc
    55681 acccgtcact taatcagcaa ctgccagata agcgaaaccg acatcccaaa cgtatttgct
    55741 gtgcgggtaa attatctgct ttatcgggca caaaaagagc gcgatgaaac attctatgtt
    55801 ggaacgcgtt tcgacaaagt tcgccgtctg gaagatgaca actggcgctt gctggaacgg
    55861 gatatcgtcc tggatcaagc ggtaatcact tcccataacc tgagtgtact gttctgatga
    55921 atcgaattta tgcgtgtccc gttgcggatg tgccggaggg tgaggctctc cggatcgata
    55981 cctcgcccgt catcgccctg ttcaacgttg gcggcgagtt ttatgccatt aacgatcgtt
    56041 gcagccatgg taatgcgtcg atgtcagaag ggtatctgga agatgacgcc acggtggagt
    56101 gcccgctaca cgccgccagt ttttgcctga aaacggggaa agcgttatgc ctgcccgcca
    56161 ccgatccgct caccacttat ccagtacacg ttgaaggtgg tgacattttc atcgacttac
    56221 cggaggcgca gccatgagcg atctgcataa cgagtccatt tttattaccg gcggcggatc
    56281 gggattaggg ctggcgctgg tcgagcgatt tatcgaagaa ggcgcgcaag ttgccacgct
    56341 ggaactgtcg gcggcaaaag tcgccagtct gcgtcagcga tttggcgaac atattctggc
    56401 ggtggaaggt aacgtgacct gttatgccga ttatcaacgc gcggtcgatc agatcctgac
    56461 tcgttccggc aagctggatt gttttatcgg caatgcaggc atctgggatc acaatgcctc
    56521 actggttaat actcccgcag agacgctcga aaccggcttc cacgagctgt ttaacgtcaa
    56581 cgtactcggt tacctgctgg gcgcaaaagc ctgcgctccg gcgttaatcg ccagtgaagg
    56641 cagcatgatt ttcacactgt caaatgccgc caggtatcct ggcggcggtg gcccgctgta
    56701 caccgccagt aaacatgccg caaccggact tattcgccaa ctggcttatg aactggcacc
    56761 gaaagtgcgg gtgaatggcg tcggcccgtg tggtatggcc agcgacctgc gcggcccaca
    56821 ggcgctcggg caaagtgaaa cctcgataat gcagtctctg acgccggaga aaattgccgc
    56881 cattttaccg ctgcaatttt tcccgcaacc ggcggatttt acggggccgt atgtgatgtt
    56941 ggcatcgcgg cgcaataatc gcgcattaag cggtgtgatg atcaacgctg atgcgggttt
    57001 agcgattcgc ggcattcgcc acgtagcggc tgggctggat ctttaaggaa gcacgatgaa
    57061 agaaaaaacg atcattattg tcggtggcgg gcaagcggcg gcaatggctg cggcctcgct
    57121 acgccagcaa gggttcaccg gtgagctgca tctgttttcc gatgagcgac atcttcctta
    57181 tgaacgacct ccactctcga aatccatgtt gctggaagat tccccgcagt tacagcaggt
    57241 gttacccgct aactggtggc aggaaaacaa tgttcatctg cattccggtg taaccatcaa
    57301 aacgctgggt cgcgacacac gagagttagt gttaaccaac ggcgaaagct ggcactggga
    57361 tcagcttttt atagcaatcg gcgcggcagc tcgaccgctg ccgttgcttg atgcactggg
    57421 agaacgctgc tttaccctac gccatgccgg tgatgccgcc agactgcgag aagttctgca
    57481 gcccgaacgg tcagtcgtga ttgtcggtgc cggaactatt ggtctggaac tggctgccag
    57541 cgccacgcag cgcagatgta aggtgacagt gattgaactg gcggcaaccg tcatgggccg
    57601 taatgcacca ccgcccgtgc aacgctatct tttacagcgc caccagcagg ctggtgtgcg
    57661 cattctgctc aataatgcca ttgaacatgt ggtcgatggt gaaaaagtag aactgacgct
    57721 gcaaagtggg gaaacgcttc aggctgatgt ggtgatttac ggtattggta tcagcgccaa
    57781 cgagcaactg gctcgcgagg ccaaccttga tactgccaat ggcattgtca ttgatgaggc
    57841 ttgccgcacc tgcgatcccg cgatctttgc cggtggcgat gtggcaatca ctcgtcttga
    57901 taatggtgca ctacaccgct gcgaaagctg ggaaaacgcc aataaccagg cgcaaattgc
    57961 cgctgccgca atgttagggc taccgctacc gctactgccg ccgccgtggt tctggagcga
    58021 tcagtacagt gataacttac agtttattgg cgatatgcgt ggcgatgact ggctttgtcg
    58081 tggcaacccg gaaactcaga aggcgatttg gtttaatctg caaaacggcg tgcttatcgg
    58141 tgcggtcacg ctgaatcagg ggcgtgagat tcgcccaatt cgcaaatgga tccagagcgg
    58201 caaaacgttt gatgcgaaac tgctgataga tgagaacatc gcgcttaaat cactgtaacc
    58261 aggataatta gcgaatatct caacgcctgg ggcgtggcga ggtgcaagag tgtgtattac
    58321 gtttaaatca cattatcttg caaagggatt ggttatgaac acacta
//
LOCUS       DAZPLI010000009        57428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZPLI010000009 DAZPLI010000000
VERSION     DAZPLI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.009"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            145..1488
                     /gene="argG"
                     /locus_tag="ACHUU3_000494"
     CDS             145..1488
                     /gene="argG"
                     /locus_tag="ACHUU3_000494"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR2080667.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1496..3121)
                     /locus_tag="ACHUU3_000495"
     CDS             complement(1496..3121)
                     /locus_tag="ACHUU3_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR2080668.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3580..3666)
                     /locus_tag="ACHUU3_000496"
     tRNA            complement(3580..3666)
                     /locus_tag="ACHUU3_000496"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3630..3632),aa:Leu,seq:gag)
     gene            complement(3681..4013)
                     /gene="secG"
                     /locus_tag="ACHUU3_000497"
     CDS             complement(3681..4013)
                     /gene="secG"
                     /locus_tag="ACHUU3_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR2080669.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4241..5578)
                     /gene="glmM"
                     /locus_tag="ACHUU3_000498"
     CDS             complement(4241..5578)
                     /gene="glmM"
                     /locus_tag="ACHUU3_000498"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR2080670.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5571..6419)
                     /gene="folP"
                     /locus_tag="ACHUU3_000499"
     CDS             complement(5571..6419)
                     /gene="folP"
                     /locus_tag="ACHUU3_000499"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR2080671.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6509..8443)
                     /gene="ftsH"
                     /locus_tag="ACHUU3_000500"
     CDS             complement(6509..8443)
                     /gene="ftsH"
                     /locus_tag="ACHUU3_000500"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR2080672.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8543..9172)
                     /gene="rlmE"
                     /locus_tag="ACHUU3_000501"
     CDS             complement(8543..9172)
                     /gene="rlmE"
                     /locus_tag="ACHUU3_000501"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR2080673.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9298..9591
                     /gene="yhbY"
                     /locus_tag="ACHUU3_000502"
     CDS             9298..9591
                     /gene="yhbY"
                     /locus_tag="ACHUU3_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR2080674.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9747..10223)
                     /gene="greA"
                     /locus_tag="ACHUU3_000503"
     CDS             complement(9747..10223)
                     /gene="greA"
                     /locus_tag="ACHUU3_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR2080675.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10471..11904
                     /gene="dacB"
                     /locus_tag="ACHUU3_000504"
     CDS             10471..11904
                     /gene="dacB"
                     /locus_tag="ACHUU3_000504"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR2080676.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            11959..12066
                     /locus_tag="ACHUU3_000505"
                     /pseudo
     CDS             11959..12066
                     /locus_tag="ACHUU3_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12090..13262)
                     /gene="cgtA"
                     /locus_tag="ACHUU3_000506"
     CDS             complement(12090..13262)
                     /gene="cgtA"
                     /locus_tag="ACHUU3_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR2080677.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13278..14243)
                     /locus_tag="ACHUU3_000507"
     CDS             complement(13278..14243)
                     /locus_tag="ACHUU3_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR2080678.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14370..14627)
                     /gene="rpmA"
                     /locus_tag="ACHUU3_000508"
     CDS             complement(14370..14627)
                     /gene="rpmA"
                     /locus_tag="ACHUU3_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR2080679.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14648..14959)
                     /gene="rplU"
                     /locus_tag="ACHUU3_000509"
     CDS             complement(14648..14959)
                     /gene="rplU"
                     /locus_tag="ACHUU3_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR2080680.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15218..16189
                     /gene="ispB"
                     /locus_tag="ACHUU3_000510"
     CDS             15218..16189
                     /gene="ispB"
                     /locus_tag="ACHUU3_000510"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR2080681.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16417..16695
                     /gene="sfsB"
                     /locus_tag="ACHUU3_000511"
     CDS             16417..16695
                     /gene="sfsB"
                     /locus_tag="ACHUU3_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR2080682.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16743..18002)
                     /gene="murA"
                     /locus_tag="ACHUU3_000512"
     CDS             complement(16743..18002)
                     /gene="murA"
                     /locus_tag="ACHUU3_000512"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR2080683.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18057..18311)
                     /gene="ibaG"
                     /locus_tag="ACHUU3_000513"
     CDS             complement(18057..18311)
                     /gene="ibaG"
                     /locus_tag="ACHUU3_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR2080684.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18471..18764)
                     /gene="mlaB"
                     /locus_tag="ACHUU3_000514"
     CDS             complement(18471..18764)
                     /gene="mlaB"
                     /locus_tag="ACHUU3_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR2080685.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18764..19399)
                     /gene="mlaC"
                     /locus_tag="ACHUU3_000515"
     CDS             complement(18764..19399)
                     /gene="mlaC"
                     /locus_tag="ACHUU3_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR2080686.1"
                     /translation="MFKRLMMVALLVIAPLSAATEADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19418..19969)
                     /gene="mlaD"
                     /locus_tag="ACHUU3_000516"
     CDS             complement(19418..19969)
                     /gene="mlaD"
                     /locus_tag="ACHUU3_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR2080687.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19974..20756)
                     /gene="mlaE"
                     /locus_tag="ACHUU3_000517"
     CDS             complement(19974..20756)
                     /gene="mlaE"
                     /locus_tag="ACHUU3_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR2080688.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20764..21573)
                     /gene="mlaF"
                     /locus_tag="ACHUU3_000518"
     CDS             complement(20764..21573)
                     /gene="mlaF"
                     /locus_tag="ACHUU3_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR2080689.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21783..22760
                     /locus_tag="ACHUU3_000519"
     CDS             21783..22760
                     /locus_tag="ACHUU3_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR2080690.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22774..23760
                     /gene="kdsD"
                     /locus_tag="ACHUU3_000520"
     CDS             22774..23760
                     /gene="kdsD"
                     /locus_tag="ACHUU3_000520"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR2080691.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23781..24347
                     /gene="kdsC"
                     /locus_tag="ACHUU3_000521"
     CDS             23781..24347
                     /gene="kdsC"
                     /locus_tag="ACHUU3_000521"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR2080692.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24344..24919
                     /gene="lptC"
                     /locus_tag="ACHUU3_000522"
     CDS             24344..24919
                     /gene="lptC"
                     /locus_tag="ACHUU3_000522"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR2080693.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24888..25445
                     /gene="lptA"
                     /locus_tag="ACHUU3_000523"
     CDS             24888..25445
                     /gene="lptA"
                     /locus_tag="ACHUU3_000523"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR2080694.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25452..26177
                     /gene="lptB"
                     /locus_tag="ACHUU3_000524"
     CDS             25452..26177
                     /gene="lptB"
                     /locus_tag="ACHUU3_000524"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR2080695.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26225..27658
                     /gene="rpoN"
                     /locus_tag="ACHUU3_000525"
     CDS             26225..27658
                     /gene="rpoN"
                     /locus_tag="ACHUU3_000525"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR2080696.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27681..27968
                     /gene="hpf"
                     /locus_tag="ACHUU3_000526"
     CDS             27681..27968
                     /gene="hpf"
                     /locus_tag="ACHUU3_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR2080697.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28086..28577
                     /gene="ptsN"
                     /locus_tag="ACHUU3_000527"
     CDS             28086..28577
                     /gene="ptsN"
                     /locus_tag="ACHUU3_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR2080698.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28623..29477
                     /gene="rapZ"
                     /locus_tag="ACHUU3_000528"
     CDS             28623..29477
                     /gene="rapZ"
                     /locus_tag="ACHUU3_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR2080699.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29474..29746
                     /gene="npr"
                     /locus_tag="ACHUU3_000529"
     CDS             29474..29746
                     /gene="npr"
                     /locus_tag="ACHUU3_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR2080700.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29960..30592
                     /gene="yrbL"
                     /locus_tag="ACHUU3_000530"
     CDS             29960..30592
                     /gene="yrbL"
                     /locus_tag="ACHUU3_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR2080701.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30589..31317)
                     /gene="mtgA"
                     /locus_tag="ACHUU3_000531"
     CDS             complement(30589..31317)
                     /gene="mtgA"
                     /locus_tag="ACHUU3_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFR2080702.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31314..31967)
                     /gene="elbB"
                     /locus_tag="ACHUU3_000532"
     CDS             complement(31314..31967)
                     /gene="elbB"
                     /locus_tag="ACHUU3_000532"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR2080703.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32197..34533)
                     /gene="arcB"
                     /locus_tag="ACHUU3_000533"
     CDS             complement(32197..34533)
                     /gene="arcB"
                     /locus_tag="ACHUU3_000533"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR2080704.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34629..35558)
                     /locus_tag="ACHUU3_000534"
     CDS             complement(34629..35558)
                     /locus_tag="ACHUU3_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR2080705.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36233..40693
                     /gene="gltB"
                     /locus_tag="ACHUU3_000535"
     CDS             36233..40693
                     /gene="gltB"
                     /locus_tag="ACHUU3_000535"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR2080706.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40706..42124
                     /gene="gltD"
                     /locus_tag="ACHUU3_000536"
     CDS             40706..42124
                     /gene="gltD"
                     /locus_tag="ACHUU3_000536"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR2080707.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42308..42712
                     /locus_tag="ACHUU3_000537"
                     /pseudo
     CDS             42308..42712
                     /locus_tag="ACHUU3_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            42804..43109
                     /locus_tag="ACHUU3_000538"
     CDS             42804..43109
                     /locus_tag="ACHUU3_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR2080708.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43185..43649)
                     /gene="nanQ"
                     /locus_tag="ACHUU3_000539"
     CDS             complement(43185..43649)
                     /gene="nanQ"
                     /locus_tag="ACHUU3_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR2080709.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43646..44521)
                     /gene="nanK"
                     /locus_tag="ACHUU3_000540"
     CDS             complement(43646..44521)
                     /gene="nanK"
                     /locus_tag="ACHUU3_000540"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR2080710.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44518..45207)
                     /locus_tag="ACHUU3_000541"
     CDS             complement(44518..45207)
                     /locus_tag="ACHUU3_000541"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR2080711.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45255..46745)
                     /gene="nanT"
                     /locus_tag="ACHUU3_000542"
     CDS             complement(45255..46745)
                     /gene="nanT"
                     /locus_tag="ACHUU3_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR2080712.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46855..47748)
                     /gene="nanA"
                     /locus_tag="ACHUU3_000543"
     CDS             complement(46855..47748)
                     /gene="nanA"
                     /locus_tag="ACHUU3_000543"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR2080713.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47870..48661)
                     /gene="nanR"
                     /locus_tag="ACHUU3_000544"
     CDS             complement(47870..48661)
                     /gene="nanR"
                     /locus_tag="ACHUU3_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR2080714.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49041..50363
                     /gene="dcuC"
                     /locus_tag="ACHUU3_000545"
     CDS             49041..50363
                     /gene="dcuC"
                     /locus_tag="ACHUU3_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR2080715.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(50406..50903)
                     /gene="sspB"
                     /locus_tag="ACHUU3_000546"
     CDS             complement(50406..50903)
                     /gene="sspB"
                     /locus_tag="ACHUU3_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR2080716.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(50909..51547)
                     /gene="sspA"
                     /locus_tag="ACHUU3_000547"
     CDS             complement(50909..51547)
                     /gene="sspA"
                     /locus_tag="ACHUU3_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR2080717.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(51942..52334)
                     /gene="rpsI"
                     /locus_tag="ACHUU3_000548"
     CDS             complement(51942..52334)
                     /gene="rpsI"
                     /locus_tag="ACHUU3_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR2080718.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52350..52778)
                     /gene="rplM"
                     /locus_tag="ACHUU3_000549"
     CDS             complement(52350..52778)
                     /gene="rplM"
                     /locus_tag="ACHUU3_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR2080719.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(52997..54124)
                     /gene="zapE"
                     /locus_tag="ACHUU3_000550"
     CDS             complement(52997..54124)
                     /gene="zapE"
                     /locus_tag="ACHUU3_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR2080720.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54318..54716
                     /gene="zapG"
                     /locus_tag="ACHUU3_000551"
     CDS             54318..54716
                     /gene="zapG"
                     /locus_tag="ACHUU3_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR2080721.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            54870..56237
                     /gene="degQ"
                     /locus_tag="ACHUU3_000552"
     CDS             54870..56237
                     /gene="degQ"
                     /locus_tag="ACHUU3_000552"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFR2080722.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56327..57394
                     /gene="degS"
                     /locus_tag="ACHUU3_000553"
     CDS             56327..57394
                     /gene="degS"
                     /locus_tag="ACHUU3_000553"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR2080723.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14256 a        15305 c        14261 g        13606 t
ORIGIN      
        1 gcgcattatt ttacaaaaaa cacactaaac tcttcctgtc tccgataaaa gatgattaaa
       61 tgaaaactca tttattttgc ataaaaattc agtgagagcg gaaatccagg ctcatcatca
      121 gttaattaag cagggtgtta ttttatgacg acgattctca agcatctccc ggtaggtcaa
      181 cgtattggta tcgctttttc cggcggtctg gacaccagtg ccgcactgct gtggatgcga
      241 caaaagggag cggttcctta tgcatatact gcaaacctgg gccagccaga cgaagaggat
      301 tatgatgcga tccctcgtcg tgccatggaa tacggcgcgg agaacgcacg tctgatcgac
      361 tgccgcaaac aactggtggc cgaaggtatt gccgctattc agtgtggcgc atttcataac
      421 accaccggcg gcctgaccta tttcaacacg acgccgctgg gccgcgccgt gactggtacc
      481 atgctggttg ctgcgatgaa agaagatggc gtgaatatct ggggtgacgg tagcacctat
      541 aaaggaaacg atatcgaacg tttctatcgt tatggtctgc tgaccaatgc tgaactgcag
      601 atttacaaac cgtggcttga tactgacttt attgatgaac tgggcggccg tcatgagatg
      661 tctgaattta tgattgcctg cggtttcgac tacaaaatgt ctgtcgaaaa agcctactcc
      721 acagactcca acatgcttgg tgcaacgcac gaagcgaagg atctggaata cctcaactcc
      781 agcgtcaaaa tcgtcaaccc aattatgggc gtgaaattct gggatgagag cgtgaagatc
      841 ccggcagaag aagtcacagt acgctttgag caaggtcatc cggtagcgct gaacggtaaa
      901 acctttagcg acgacgtaga aatgatgctg gaagctaacc gcatcggcgg tcgtcacggc
      961 ctgggcatga gcgaccagat tgaaaaccgt atcatcgaag cgaaaagccg tggtatttac
     1021 gaagctccgg ggatggcact gctacatatc gcttatgaac gtctgttgac cggtattcac
     1081 aacgaagaca ccattgagca gtatcatgca catggtcgtc agttgggccg tctgctgtac
     1141 caggggcgtt ggtttgattc ccaggcgctg atgctgcgtg actctctgca acgctgggtt
     1201 gccagccaga tcactggtga agttaccctg gagctgcgcc gtgggaacga ttattcaatc
     1261 ctgaataccg tctcagagaa cctgacctac aagccagagc gtctgacgat ggaaaaaggc
     1321 gactcggtgt tctcgccaga tgatcgtatt ggtcaattga ccatgcgtaa cctggatatc
     1381 actgataccc gagagaaact ttttggttat gccaaaactg gcctgctctc ctcctctgcc
     1441 acttcaggcg tgccgcaggt ggagaatctg gaaaacaaag gccagtaatt cgacatcaac
     1501 cctgcccttc aggggtgggg ttaccttgct tatcctctgt aaactccttt ctgagcatca
     1561 cataatcgaa aacatcatgg tttgcatcca caacccgtga gtctccttta tagtgtgcaa
     1621 tcacttcctc cagcgttttc ggttgttccg gctttgttac ccccatccac gcattaataa
     1681 ggtaattatt gtaagctgtc gaaaagatgt tgttttccgt tgtacgatcc acgccatccc
     1741 caagaacggg gctataccag ataaacatcg ggacatgata tgcctgctgg ctagcctccc
     1801 tgcctccatg aaaatagacg ttcttcttcg tagggtcacg ttccaggccg tggtcggcaa
     1861 aatacatgac cgaggcgcgg cgatctttta ataattcaaa aacctgacct agcaaactat
     1921 ctgtgtaatg aatggagttg tcatagcagg catcctgatc gtcctgcggt tgaaacacgg
     1981 cgctggattg cggataggcg ctacaagccg gttcatggct tccatttaaa tgaagaacaa
     2041 tcagtttctt ttgctgcgta ttttgctgta atgcttgcga taaatgcggc aacaacaatt
     2101 catcaaatcc tctgacataa actgtttcca tggcgcgcat ggcgatactg gtaactgctg
     2161 taccattctg ccgaaaagcg gattgcgagc ttagccagaa agtctgaaat cctgcttgat
     2221 tagccatatt aataatgttg tcggggtaat tatgaatgtc atgactcaaa acagaatcag
     2281 cagttaaaga aaggggaacc gacagcgcag tgtaaggtgc gccgcttatt gattgattaa
     2341 acagtttgat ctgttttctt tgtgcttcaa cttgcggtgt cgtagagcgt gtatatccat
     2401 acaaagacat attgtcgaca cgtacagact cccccacaat caacacgtag gtatcaatac
     2461 ctgtatccct gactgataat tgaaaatacg gcaccgtgtt tgcaattgag agtaatcttt
     2521 gatgctcttt cgctgctaaa gcaaaataat tgagattgaa aaacggcgta taggtagcaa
     2581 atcgcgacgc tagtatatat ggactgaatg cttttttatt ttttgcatct ttataagcaa
     2641 attgacaagc agaaaataaa ctgcccgaaa tgacaatcag caataatatt cctgtcactt
     2701 tttttgtcgg caaggaaaca tcatatttta ttattactgc caaaaaaaga agggataaaa
     2761 aagcaaaagc acatagataa gggatataca tccccagcat tttgacaact tcatccggat
     2821 cactctgcag cacactaatc gcaaatccat cattgaaagt tgtaccaaag gtacaccatg
     2881 aatagaggct aatactcatg tctgccgcag tcagtaacac aaatggtgcg gcaacaagca
     2941 ggcgaagcgg aagggatgat atacgtttta caaccaggat ggtcaggacg aagaacagca
     3001 atcgctgaac catatgcgcg ccggaagagg cgactaagtt cgtaatacca aaaacaataa
     3061 cacaaagata caacaaccaa tgagatttaa aacttctagc aaatttgttg aatactgtca
     3121 tccagaaatc caagttcaca accaatataa acaattatca acaatgttca attttatatc
     3181 agacatgttt ttcaactctg atccatatca gaccgaaaca ctgttagttt tttcttttta
     3241 aatgatgcgg ccattgcgtt ctcagtaatt aaataacatt atcaatgcgt ctctaaacat
     3301 tacgcagaac gtagccatca gtagttatac tgacaggagc aggatggttt atcgagaagt
     3361 aaacatatcc gtattcgtac cggaactgga gttgacacaa taaagtgcca attatgtcag
     3421 cagaagggaa aagtttataa ctaaggcgta ccggcaccat cgtttcaagg taccagctac
     3481 gagtaaagca actggacgag atacagatac ctgacaacca ttcctcagac caggaccaaa
     3541 acgaaaaaag acgcttttca gcgtctcttt tctggaattt ggtaccgagg acgggacttg
     3601 aacccgtaag ccctattggg cactaccacc tcaaggtagc gtgtctacca attccaccac
     3661 ctcggcacgg atactacttt ttagttcggg atatcgctgg tcggcttagc cggagcagct
     3721 ggctgagttt gttcggtttt cgccggtgca ctcagatttt cccattcgct acctttattg
     3781 gttttgttgc tattgatgtt acccagcacc agactgatga tgaagaataa cgttgccagc
     3841 agcgccgtca tgcgggtcat gaagttacca gaaccacttg aaccaaacag cgtagcggaa
     3901 gcgcctgctc cgaaggaggc tcccatatca gcgcctttac cttgctgcag catgatcaga
     3961 ccaacaaggc caattgccac aataaggaaa actaccaaaa gagcttcgta cataatcaac
     4021 ctgttccttg cggagttgcc gcgtaccaat gcttcaacca ataaagcggg agtttttaat
     4081 ctttcccact gaagtgggtg tgaatactaa ccaaagcgaa tgaccttcgc aagggcaatt
     4141 ttatcgcatt gtatcaactg cggaaaaaaa cagcaaaacc gatgtgttcg ctgagaaaaa
     4201 aggcgacaat cgccgccttt ttagccagtt atttaccgtt ttaaacggct tttactgcat
     4261 cggcgatacg gtgtgcaaat tcagttacct gcgcttcgtc ttcaccttct accatcacgc
     4321 gaattaacgg ttcggtgccg gatttacgca acagcacacg tccacggcta cccagcgccg
     4381 cttcaacttc ggcggtcaca gctttaactg actcatgctc aagcggatcg ccgctacctg
     4441 cggtgtaacg cacgttaacc agaatctgcg ggaacatttt catgccgctg caaaggtcgt
     4501 gcaggctcat atggttacgt gccatcgccg ccagcacctg caagccagca acgatgccgt
     4561 caccggtagt ggttttatcc agcaggatca catgaccgga attctctgca ccgatacgcc
     4621 agcctttctc ctgcattttt tccagtacgt agcggtcacc cactttcgcg cgcgcaaatg
     4681 gaattcctaa ctgtttcaac gccagctcaa gccccatgtt actcatcaat gtacccacag
     4741 caccaccacg cagctggccc tgacgaagac cttcacgcgc gatgatatac atgatctgat
     4801 caccatcgac tttattgcct tcatggtcaa ccataatcac gcgatcgccg tcgccgtcga
     4861 aggcaatacc gagatccgct ttttcagcca gcacacgagc ctggagcgcg cgaacgtcgg
     4921 tagcccccac ttcggcattg atgtttacac cgttcggctc acaaccgata gcgataacgt
     4981 tcgcccccag ttcacgcagc acgttcggcg cgatgtgata agtcgcaccg tttgcgcaat
     5041 ccaccacaat cttcagttcg ctgaggctaa gttcgttcgg gaacgtggct ttgcaaaact
     5101 cgatatagcg acccgcagca tcaacgatac ggctggcttt acccagttct gccgaatcaa
     5161 cacagctgat ctctttttcc atttccgctt cgatggcctc ttctaccgca tccggtagtt
     5221 tggtgccgtc gatagagaaa aatttaatgc cattatcgta gaacgggtta tgcgatgcag
     5281 atatcacaat tccggcctct gcgcggaagg tacgcgtcag ataagccacg gccggtgttg
     5341 gcatcgggcc agtgaagagt gcggaaaggc ccgctgccgc cagacccgct tccagtgctg
     5401 actccagcat atagccagaa atacgcgtgt ctttaccaat aatgatctta cgggagccgt
     5461 ggcgcgccag cactttaccc gcggcccaac ccagcttaag cacaaaatca ggtgtgatcg
     5521 gcgcatcccc tacacgacca cgaatcccat cggtaccgaa atatttacga ttactcatag
     5581 cgtttgtttt cctttgcaga cagagtggct tccaccaccc gcatcgcttc tacggtttct
     5641 ttgacgtcat gaacacgaat aatgtgcgcg ccttgcattg cggcaatgac cgcacaggcc
     5701 agactaccgc tcaggcgctc ggacggcccc acgttcagca gctgcccaat catcgatttt
     5761 cgtgacatac ctaccaacag cggcaggttg aaatggtgaa attcagccag gcgcgccagt
     5821 aatgaatagt tatgggagag atttttaccg aaaccgaatc cggggtcgag caacaatttc
     5881 tcttttgcga tacccgcctg ctcgcaacgt gctatttgct caataaagta gcgattcact
     5941 tctgcaaaga catcgtcata cttcggagct tcctgcatgg tttttggatt tccctgcata
     6001 tgcatcagac aaaccggtaa accggtttct gcagccgcct ccagagcgcc aggttcggaa
     6061 agggagcgga tatcgttaat aatgtgagcg ccaactttcg ctgactcacg gatgacttct
     6121 ggtttggatg tatctaccga gatccagact tcgaagcgtt gagcaattgc ctcaaccaca
     6181 ggaataacac gttgcaactc ttcttcaacg ctaacttccg ccgcccctgg gcgcgtggac
     6241 tcgccaccaa cgtcaatgat cgtcgcgcca gcgttgatca tcagattcgc atgtttcacc
     6301 gcatctatca gcgagttatg cgtgccacca tccgaaaagg aatcaggcgt gacgttgagg
     6361 atccccatta cgtgaggatg gctaaggtcc agtgaagtgc cctgggcaaa gagtttcatg
     6421 gtgttatccc tggtatgaat tgataagaaa aaaccccgga gcacgccccg gggttttcgg
     6481 tacaaataca gtcatctgat gcgggaactt acttgtcgcc taactgctct gacatggtgt
     6541 tacccgggtt cggcgtacgc ggttcatcaa ccggacgagg agcctttgga ctaccattgt
     6601 cgccagaatt gttagaagcg cctggttctt cccagcccgc tggcggacgt acatcgcgac
     6661 gtgccatcag gtcatcaatc tgcggtgcgt cgatagtctc atatttcatg agagcatctt
     6721 tcatcgcatg cagaatatcc atattgtcgg tcagaagctg acgcgcacga ttatagttac
     6781 gctcaatcag tgctttcact tcctggtcga tgatacgtgc agtttcatcg gacatatgtt
     6841 tcgctttcgc tacgctacgg ccgaggaaca cttcaccttc ttcttccgcg tacagcagtg
     6901 gacccaattt ctcagagaag ccccactgag tcaccatgtt acgcgccagg ttggtcgcaa
     6961 ctttaatatc gttggacgca ccggtagaaa catgttccgg cccgtagatg atctcttctg
     7021 ccagacgacc accgtacagc gtagaaatct ggctttccag tttctgacgg ctggcgctga
     7081 ttgcgtcgcc ctcaggcaag aagaaagtca cacccagcgc acgaccgcgt gggataatcg
     7141 tcactttgtg caccggatcg tgttccggca ccaggcgacc gataatcgca tgacccgctt
     7201 cgtggtaagc cgtcgattct ttctgcgctt ccgtcatcac catggagcga cgttccgcac
     7261 ccatcatgat tttatctttc gctttctcga actcaaccat cgacacaacg cgtttgttgc
     7321 cacgagcagc gaacagtgcc gcttcgttta ccaggttcgc caggtcagca ccggagaaac
     7381 caggagtacc acgggcaatg attgccgcgt cgatatcggg tgccaatggt acgcgacgca
     7441 tgtgaacttt caggatctgc tcacgaccgc gaacatctgg caagccaacc acaacctgac
     7501 ggtcgaaacg gccaggacgc agcagggccg ggtcaagaac gtccggacgg ttagtcgcgg
     7561 cgataacgat gataccttcg ttaccttcga agccatccat ctcaaccagc atctggttca
     7621 gagtctgttc acgttcatcg tgaccaccgc ccagaccagc gccacgctgg cggcctacgg
     7681 cgtcgatttc atcgataaag atgatgcacg gtgccgcttt cttcgcctgt tcgaacatgt
     7741 cacgaacacg ggatgcaccc acaccgacga acatttctac gaagtcagaa ccggagatag
     7801 taaagaacgg aactttcgct tcgcctgcaa tcgctttcgc cagcagcgtt ttaccggtac
     7861 ccggaggacc gaccatcagg acgcctttcg ggatcttacc gccgagtttc tggaagcggc
     7921 tcggctcgcg cagatattca accagttcag caacttcttc ttttgcttcg tcgcagcccg
     7981 caacgtcagc aaaggtcgtt ttgatctgat cttccgtcag catgcgcgct ttgctcttac
     8041 caaacgacat ggcacctttg ccaccgccgc cctgcatttg acgcatgaag aagatccaca
     8101 caccaatcag caacagcatc gggaaccagg agatgaagat agaagccagc aggcttggtt
     8161 cttcaggcgg ttcaccgaca accttgacgt tcttggtcaa caggttatcc agtaatttcg
     8221 gatcctgaac cggaatgtaa gtggtataac ggttactatc tttcttggta acgttgattt
     8281 cacgtccgtt gatacgcgct tcacgaacct ggtcgttatt gacctcttgt aggaaggtag
     8341 agtaatccac cttacggcca ttagactcgc tgggcccaaa gctctggaat actgacatca
     8401 gcacaacggc aatgaccagc cagagtatta ggtttttcgc catgtcactc aagggattaa
     8461 cctcttatta caactgtgtt aaaaacagcg tcaggatact ctatatccag catctttcaa
     8521 actttcgtct gaaatctccc ggttagggtt tacgcccggt cgctacaata tacacttccc
     8581 gcgaacgtgc acgagaagag tccggcttac gaactttgac cttcgtaaac agggagcgaa
     8641 tttcccttag atactcatcg aaaccttcgc cctggaacac cttcactaca aaactgccac
     8701 ctggcgctaa tacatcacga cacatttcta gcgccagttc caccagatac atggcacggg
     8761 ggatatccac cgccggtgtt ccgctcatgt ttggtgccat atcggacatg acaacctgga
     8821 ctttgctgtc gccaacgcgc tccagcagtg ctttcatcac cagttcatca cgaaaatcgc
     8881 cctgaagaaa gtccacacca acgataggat ccataggtaa aagatcgcaa gcgatgatgc
     8941 ggcctttgcc gccaatttgg gtgaccacat attgtgacca accacccgga gcagcaccaa
     9001 ggtcgacaac cgtcattccc ggtttaaaga gtttgtcact ttgctgtatt tcatcaagtt
     9061 taaaccaggc acgggaacgt aacccctttt tctgtgcctg ttgaacatat ttatcgctaa
     9121 agtgttcctg aagccagcgg ctggagctgg cagaacgctt cttacctgtc atttaacttt
     9181 cccatgggga taactcatcg taaccaattg cgtaaatttt tacgcgccta tttggtgata
     9241 tatgggagat ggcggtagaa tgacccattt tcaatcccaa cgtaagcaaa aaatacgatg
     9301 aatctgagta ctaaacaaaa acagcacctg aaaggtctgg cacatccgct caagccagtt
     9361 gttctgcttg gcagtaatgg tttgaccgaa ggggtgctgg ccgagattga acaagcgtta
     9421 gagcaccatg aactcatcaa ggtgaaaatc gccaccgaag atcgcgaaac aaaaaccttg
     9481 atcgtggaag ctatcgtgcg cgaaaccggc gcctgtaatg tacaggtcat cggtaaaacg
     9541 ctggtgcttt atcgcccaac taaagaacgt aaaatctcgc tgccacgcta agattatcct
     9601 aaagttacac acatttgctg tgtaaaacga ggggttttcc gcaggcagga gagcaaaatg
     9661 ccacgctctg ttcgttgata aaaggccgca tagcggcctt tttcctttct ttacaataca
     9721 tcaacatctt gagtattggg taattcttac aggtattcca ccttaattac ttcaaattct
     9781 acttcgccgc ccggcgtttt gatgaccaca acatcatctt cttctttgcc gatcaggcca
     9841 cgagcaatag gcgagtttac agaaatcagg ttttgtttaa agtcagcttc gtcatcgcca
     9901 acgatgcgat aagtctgttc ttcgtcagaa tccagattca gcaccgttac ggtagcacca
     9961 aaaataacgc gcccattgtt aggcattttg gtgacatcaa tcacctgcgc gtttgacagc
    10021 ttggcttcga tgtctttgat acggccttcg cagaagccct gctgttcacg agctgcgtgg
    10081 tattcggcgt tttctttcag gtcgccatgc tcacgcgctt ccgcgatagc agcaatgatt
    10141 tcaggacggc gcacagattt cagaaaatcc agctcttcgc gtaatttttc agcgccgcgt
    10201 aaggtcatcg gaatagcttg catttgttat acctcttgaa tattcctgat ggggcaagtc
    10261 ttcacccacc ccggctgttc ggcctgcccg gcataactcc tcaccgggac cagaagcaaa
    10321 aaaataccga cccaggtaca agtcccaggt cagctacaat tcacattttg atagtcattt
    10381 taccctgaag ttcccgaagg gtcatcgttt actttatagg gcgttgcgcc gtagtatgac
    10441 ggctcgattc caggttgtta gcgcgagatt atgcgatttt ccagatttat catcggattg
    10501 accagctgta tagcgttcag tgttcaggcc gcaaatgttg atgagtacat tactcaactc
    10561 cccgctggtg ccaaccttgc cctgatggtg caaaaagtcg gcgcgtcggc ccccgctatt
    10621 gattaccaca gtcagcagat ggcgctgcct gccagtaccc agaaagtgat tactgcgctg
    10681 gcggcgttga ttcaactcgg ccccgatttt cgttttacca cgacgcttga aaccaaaggc
    10741 aatgtggaaa acggcgtact taagggtgac ttagtggcgc gatttggtgc cgatccgacg
    10801 ttaaaacgtc aggatattcg caatatggtc gcgactttga aaaaatctgg cgtcaaccaa
    10861 atcgatggca atgtgttgat agatacctcc attttcgcca gccacgataa agcccccggc
    10921 tggccatgga atgacatgac acaatgcttt agcgctccgc ctgccgccgc catagttgac
    10981 cgcaactgtt tctccgtctc gctctacagt gcccaaaaac ccggtgatat ggcctttata
    11041 cgcgtggcat cttattaccc cgttacgatg ttcagccagg tacgcaccct cccccgtggt
    11101 tctgccgaag cgcaatactg cgaactggat gtcgtaccag gcgacctgaa ccgctttacg
    11161 ctgacgggat gcctgccaca acgttctgag ccgctcccgt tggcttttgc cgtgcaggat
    11221 ggagccagtt atgccggtgc aattctgaaa gatgagttaa aacaggcggg tatcacctgg
    11281 agcggaacac tgctgcgcca gactcaggtt aacgaacctg gaacggtagt tgccagtaaa
    11341 cagtcggccc cgctgcacga tctgcttaag attatgctga aaaagtcgga taacatgatc
    11401 gccgatacgg ttttccgcat gataggccat gcgcgcttca atgtacctgg tacatggcgg
    11461 gccgggtcgg acgctgtgcg tcagatcctg cgccagcaag ccggtgtcga tattggaaac
    11521 accattattg ccgatggttc agggctttcg cggcataacc tgattgcccc cgccaccatg
    11581 atgcaggtgc tgcaatacat tgcccaacac gacaatgaac ttaactttat ctccatgctg
    11641 ccgctggcgg gctatgacgg ctctttgcag taccgtgcag gtctgcatca ggcgggcgtg
    11701 gatggaaaag tctcagcgaa aaccggttcg ttgcaggggg tatataacct ggcgggattc
    11761 attaccacag cgagcgggca acgaatggcg tttgtgcaat atctttctgg ctatgcagta
    11821 gaacctgcgg atcagcgtaa tcgccgtatt ccgttagtgc gttttgaaag ccgtttgtat
    11881 aaagatattt atcagaacaa ttagtcaaaa agaaaccccg gcacatggcc ggggcttcag
    11941 attattgaca aagtgcgctt tgtttatgcc ggatgcggcg taaacgcctt atccggccta
    12001 caaaatcgtg caaattcaac atattgcaat tctcttgtag gcctgataag cgtagcgcat
    12061 caggctgatt tggcgtttat catcagtgat taacgcttgt aaatgaactc aacgccttct
    12121 tcgtcgtctt cgtcccagtc gtcatcccag tcttcatcat cctcttcagc aatctcttca
    12181 agctgctggc gatgataatc gtcccacatg aattcgactt tctctggctg tttcgcttct
    12241 tcagcctgca cgaccgggtt ttcaaggata aaggtcatca catcccagca gagatctttc
    12301 acgcccagtc cgctcgccgc agagatcaga taatatttat cttcccagcc cagcgcttca
    12361 gcaatcgctt tcgctttctc ttcagcttct gccttatcca gcagatcgat cttattgaac
    12421 actaaccaac gcggtttcgc cgccagatcc tggctgtatt tttccagctc gctgataata
    12481 atacgcgcgt tttcaaccgg atcggtgccg tcaatcggat cgatatcgat gaggtgcaac
    12541 aggacgcggc aacgttccag gtgcttcagg aagcggatac ccagacctgc gccttccgca
    12601 gcgccttcaa tcagtcctgg aatatcggca acaacgaagc tcttttcgtt gtccattcgt
    12661 accacaccca gacttggcac cagagtggta aacggataat ccgccacttt cggtttagcc
    12721 gccgataccg cgcgaataaa ggtcgattta cccgcgtttg gcatccccaa cataccgacg
    12781 tcagccagca gcatcagctc cagcagcagc tcacgcttat cacccggcgt accgttggtt
    12841 ttctgccgtg gtgtacggtt aacggacgat ttgaaacggg tattgcccag accgtgccag
    12901 ccgcccttag caaccagcag acgctgaccg tgtttagtca tatcgcccat ggtttcacca
    12961 gtaccctggt cgattacacg cgtacctacc ggcactttaa tcgtcacgtc tttaccgcgc
    13021 ttaccggtac aatcgcggct tgcgccgttc tgaccgcgct ctgcacggaa agatttttca
    13081 aaacgataat cgataagcgt gttcaggttt tcgtcggctt ccatccatac gtcaccacca
    13141 tcaccaccgt cgccgccatc cgggccgcct ttcggaatat acttttcgcg gcggaagctc
    13201 acgcaaccat taccgccatc acctgcaacg accagaatcg atgcttcatc aacaaacttc
    13261 attttattct ccataaatca ttcgcctgcg cggggttgcg ataccaccgt tgtatgctta
    13321 cgtaatccgc cccaaatacg atgaccaatg gcggaataca tcgcgcccgc aaccacgaca
    13381 aacgcaccga gataacctaa aaggtttaac atcggtctgg cgaagaaatc gggccaggcc
    13441 agtgataaaa gatctgaaaa aaacagcgta aacagtgggg tgagcgtgat gatcgcgctc
    13501 acctgcgctg cctgccagcg agccatcgct tccgccaggg cgccatatcc taccaatgta
    13561 ttcagtccgc aaaaaattaa acatgcgagc tgccagtggc taagctgcgc tatcactcca
    13621 ggctttgcca gagggaagag cgcaattgta cataaagtgt acagtaaaaa caggatctgc
    13681 ggtgaggcca gccgacgcaa taaaaccttt tgcgccacgc cataactcac ccaaaccgtc
    13741 gccgcaccga ccccaaagat aactccccag gtgtaatcgg tgagctttgt aaatatctcg
    13801 accagactgg tgttaaaaaa catcaccagg ccgctcagga gcatcaatgc ccctacaacc
    13861 tgagtgctgc gcattttctc tttcaggata aatacgctgg caaccatcat gccaactggc
    13921 gagagttgcc caatcacctg cgaagcggtc ggactcaggt attgcaagga tgagctgaac
    13981 aggatgaagt tcccaaacag cccggcggtc gccactgcca acaaaatcaa ccagcgtggc
    14041 ttacgaaaca cgcgtaatgg cggcaacctc ttcttcaccg caagaatggc accaaggcca
    14101 atactcgcca tcaagaaacg gtaaaacacg attgtcggag gttccatcac ctccagcacc
    14161 tgcttcattg cgattggcaa cgccccccag caaattgctg tggtgagcgc caaaagaatg
    14221 ccaatgcctg cctgctgctt cataccgtat tccctgcaaa gcgcatttgc ggattaacgt
    14281 cgcaccatgc gcatttttac cggttatcga atgtaaaaag ccccgcaacg tgttgcgggg
    14341 ctttcatccg ttaccgggac gcgaaaaact tattcagctt cgatgctgat aaatttacgg
    14401 tttttcgggc ctttaacttc gaatttcact ttaccgtctg ctttagcaaa cagagtgtgg
    14461 tcacgaccgc aacctacgtt agcgccagcg tggaatttgg taccacgttg acgaacgatg
    14521 atgctacccg ccagaacgga ttcgccaccg aaacgcttaa cgcccaggcg tttagcttct
    14581 gaatcgcgac cgttacgtgt ggagccgcca gcctttttat gtgccatttg aaatctctcc
    14641 tcaggtctta ggcgctgatg ccagtaattt tcacatcagt gaaccactga cgatggccct
    14701 gctgcttacg atagtgttta cgacgacgaa acttaacgat tttaactttc tcgccacgac
    14761 cgtgagcaac aacttcagct ttgattacgc cgccatcaac gaaaggaacg ccgattttga
    14821 cttcttcacc gtttgcgatc atcagcactt cagcgaactc aacagtttcg ccagttgcga
    14881 tgtccagctt ttccaggcga acggtctgac cttcgcttac tcggtgttgt ttaccaccac
    14941 tttggaaaac cgcgtacata taaaactccg cttccgcgca caccttttca atgattcaga
    15001 gtgcgctata aatattcaca atagggcgcg aatattacgc aaaacgcacg cctttgacaa
    15061 gtgctacagt caatacacga agaaaaaaaa cacaacttgt acggtaacgt ttatctgtgc
    15121 cattttttca gtacaatcac cctatattcc taaccataaa ccctaagttg cctttgttca
    15181 cagtaaggta atcggggcga aaagcccggc ttttgcgatg aatttagaaa aaatcaatga
    15241 gttaaccgcg caagatatgg cgggtgttaa tgcggcaatc cttgagcagc ttaattccga
    15301 cgtccaactg atcaatcagt taggctatta catcgtcagc ggcggcggta aacgtattcg
    15361 tccgatgatt gctgtactgg ctgcacgagc tgttggctat gagggaaatg cgcatgtcac
    15421 cattgctgcc ctgatcgagt ttatccacac ggcgactctg ctacacgacg acgttgtgga
    15481 tgaatcagat atgcgcaggg gtaaagctac cgccaacgcc gcatttggca atgccgccag
    15541 cgtgctggta ggcgatttta tttatacccg cgcgttccag atgatgacca gcctcggttc
    15601 actcaaagtg ctggaagtca tgtcagaagc tgtaaacgtc atcgcagaag gtgaagttct
    15661 gcaactgatg aacgttaacg atccggacat cactgaagaa aactacatgc gcgttatcta
    15721 tagcaaaacc gcgcgtctgt ttgaggctgc cgcgcagtgt tccgggattc tggctggctg
    15781 tacgccggag gaggagaaag gcctgcagga ttatgggcgc tatctcggca ctgctttcca
    15841 gttgatcgac gatttactcg attacaatgc cgatggcgaa cagttaggta aaaatgtcgg
    15901 cgacgatctg aacgaaggta aaccgacgct gccgctgctg catgcgatgc atcatggcac
    15961 accagaacag gcacagatga tccgtaccgc catcgaacag ggtaacggtc gccatcttct
    16021 ggaaccggtt ctggaagcaa tgaacgcttg tggatctctt gaatggacgc gtcagcgtgc
    16081 cgaggaagaa gcagacaaag ccatcgcagc attacaggtg ctcccggaca ccccttggcg
    16141 agaagcactc atcggcctcg cgcacatcgc tgttcaacgc gatcgttaat cccctccctt
    16201 catcccgcgc agtgcgcggg atgattccag taaattccat aaaaacacta attcagcgct
    16261 ataacacctg aaaaaaaggg cgatgaatct ttatatattc tgaatattca cactctttac
    16321 aggaactttt tagagcaata ggccatcagg agtatagtga tgcccgacag aagaagtgtt
    16381 ctgaatgaaa gcgactaact taaggagtga ggaaagatgg aaagtaattt cattgactgg
    16441 catcccgctg acatcattgc gggtttgcgc aagaagggaa cgtcaatggc ggcggagtct
    16501 cgcagaaatg gtttgagttc ctcaacgctg gcaaacgcat tatcgcgccc atggccgaaa
    16561 ggagagatga ttattgcgaa agccctggga actgacccct gggttatctg gccatcacgc
    16621 taccatgatc cgcagaccca tgagtttatc gacagaacgc agttgatgcg tagctacact
    16681 aaaccgaaaa aatgagtggt ctggcggtag ccccgcgaac ggggctgcca gctctcagac
    16741 gattactcgc ctttcacacg ctcaatattt gcacctaaag cgcgcagttt gtcttcaatg
    16801 cgttcgtagc cacgatcgat gtgataaata cgatcaacca ccgtcgtccc ttccgcaata
    16861 cagccagcca gcaccaggct tgctgatgca cgcagatcgg ttgccataac ctgtgcgcca
    16921 gaaagttttt caacaccgtg acaaataacg gtattgcttt cgatttcggc atgcgcgccc
    16981 atacggctaa gctccggcac atgcataaag cggttttcaa agaccgtttc ggtgatgaat
    17041 ccggtccctt ctgccaccag gttcaacagc gtgaactggg cctgcatatc ggtcgggaat
    17101 gccggatgcg gcgcggtacg tacgttaaca gccttcggac gtttgccatg catatccagg
    17161 ctaatccagt cttcgccgac ttcgatgtcc gctccagcgt cacgcagttt cgccagcacg
    17221 gcgtcgagag tatctggctg cgcgttacgg cagataattt tgccgcgaga aatcgccgcc
    17281 gccaccagga aagtaccggt ttcgatacga tccggtagca cgcgatagac accgccgcct
    17341 aaacgttcca caccttcgat gacgatacga tcggtgccct gaccgctaat tttcgcaccc
    17401 agcgtaatca ggaagttcgc ggtatcgacg atttccggtt cacgcgctgc gttttcaata
    17461 atcgtggtac cttccgccag ggttgcagca cacatgatgg tcaccgttgc gccaacgctg
    17521 actttatcca tcacgatatg tgcgcctttc agacgaccat cgacggaagc tttaacgtaa
    17581 ccttcttcca gtttgatggt cgcgcctaat tgttcgaggc cagaaatgtg tagatcaacc
    17641 ggacgcgcac cgatcgtaca accgccaggc agtgaaactt gcccctgacc aaagcgcgct
    17701 accagcggcc ccagtgccca gatagaagca cgcatggttt taaccagatc gtaaggtgcg
    17761 cagaatacat taacgtcgcg ggcatcaata tgcacagaac cattacgttc tactttcgca
    17821 cccagctggc ttagcagctt cattgatgta tcgacgtctt tcagttttgg gacgttctgg
    17881 atctctaccg gttcttccgc cagtagagcg gcaaaaagga taggcagagc agcattttta
    17941 gcgccggaaa ttgtgacttc gccctggagc ttcgttggcc cctgaacacg aaatttatcc
    18001 atttagtttg ttctcagtta acaattcata tccgctaccg gcgaatcgcc catagctcaa
    18061 aagccgttca gtttgcgatc gcgcgcccac tccgcagggg tatacgcttt gatcgacaca
    18121 gcatgaatgc ggttatccgc aatatattcc atcagcgggc catagaccgt ctgctgtttt
    18181 ttaacccgac tcatgccgtc aaacaactca cccacggcaa taacctgaaa gtggctgcca
    18241 tcgccggaaa cgtggacttc ctggagggag agagcgttca tcaacacgct ctgaatttca
    18301 ttattttcca tgggatcttc aatcatcagt taataaacca gcgaaacatc ttagagcaaa
    18361 gttgcgctgg cataaataag caaaaagcct cgctgataaa tcagacaagg ctcgacttgc
    18421 aggcaggttt gccggacagg cggttaacgc catatccggc ctgaaaaaat ttaacgaggc
    18481 agaacatcag caggcaaatt atacaatttc gcgagggtat acactttgtc gtttaccccc
    18541 tgaagcgtca cgttgttgcc ctgctttttc gccagatcga taagatggag cagcagtgcc
    18601 agtccacccg tatccacgcg ggacacacgg ctaagatcga tgcaggtaat ccccttcacc
    18661 gcttcctcac gcatttccca aagcggtagc agaacgtcct gatccagctc tccggataac
    18721 gccagcgtgt cacccgtctg catccagctc agtgactcgc tcattatttt ttctcttcca
    18781 gagtgatttt ctgttgagaa atcgatttca gttgcgcagt caggccgtcg atacctttgg
    18841 tacgcagcag cgttccccac tcgttttgtt tggtggtgat catactgacg ccttcagcaa
    18901 tcatgtcgta agcctgccag tttcccgtct gggagttttt acgccactgg aagtccagac
    18961 gcaccggcgg acggccattc gggtcaataa tggtaacgcg aataggcaca atggttttat
    19021 cgcccagcgg ctgttctggc gcaatctgat aggtttgacc gtgatacatc gccagcgcct
    19081 gaccgtaagc ctgcttcagg tactcacgga aagcggcaaa gtaggcttca cgttgagcag
    19141 gggtcgcact cttgtaatac tggcccagca ccagcgcacc ggcgtatttc acctgtacgt
    19201 atggcagcag ttcctgatca acaatggtac gcagataatc cgggttggcc cgaatttgcg
    19261 gttgctcatt cttcaggcga tcgaacgttt tctgcgccgc ctcgtccatc agcttataag
    19321 gattggtctg gtctgcctcg gttgccgcac tcagaggtgc aatcaccagc aaagcgacca
    19381 tcattaaacg tttaaacatg cgtcggttct cctgaaatta tttcgttgta cccacaggtt
    19441 cagtggtttc attattacct ggcgcagcag ctggcgcatc gccactattc ttattgtcat
    19501 cgcctttact accgtaaagg aactgaccaa tgagatcttc cagcaccatc gcagacttag
    19561 tgtcctgaat tgtatcgcca tccttcagga tagcagtccc cagttccggg tcttcaaaac
    19621 cgacgtttaa tgccagatat tgttccccca gcaggccgga agtacgaatg ctcagcgaac
    19681 tggtatctgg aatgtggtta taacgttgtt caatttccag cgttacgcgc ggcagatagg
    19741 ttttcgggtc cagcgtaata tccgccaccc gacccacaac aacgccacca atactgaccg
    19801 gagagcgcgc tttcaggccg ccaatgttat cgaacgtcgc ataaagcgtg taggtcggtt
    19861 cagtacgtat ggacgtcacg ttcgccgcct tcaggcaaac aaacagcgcc gccagcagtg
    19921 ctgctaataa aaagataccc acccaaattt catttttttt cgtttgcatg aactcaattc
    19981 ccaaacatca atgcggtcag cacaaaatcc agccccagaa cagccagaga cgagtggaca
    20041 acggtgcgag tggttgcccg gctaatcccg gcagacgtcg ggatggcgtc gtagccgtta
    20101 aacaacgaaa tccacgtcac cgtgatggcg aacaccacgc tcttaatcag acagttgacc
    20161 agatccatac gccagtcgac ggcattttgc attgccgacc agaagaaccc gctatcaatg
    20221 cctttccaac tgacgccgac cagagatccg ccccagatcc ccacggcgac gaaaataacc
    20281 gtcaacagtg gtaatgaaat aaccccagcc cagaaacggg gagaaataac ccgacgcagc
    20341 ggatccaccg ccatcatctc catactggag agttgctctg tagcgcgcat caggccgatt
    20401 tctgcggtta gcgccgaacc agcacgcccg gcaaacaaca acgcggcaac caccggcccc
    20461 agttcacgca gtagcgataa cgccaccagc atacccagac tggtttccgc actataagtg
    20521 gtcagaacca gataaccttg cagccccaac accattccga tgaacacgcc agaaaccaca
    20581 ataatcagca tcgacaggac gccgacatta tagagctggc gcaccagcag cggcgcatgt
    20641 ttgcgaaatt ccggtttgcc gaccagcgca ttgaataaca ttaacccggc ccgcccgaac
    20701 gttctcaggg ttttaatccc tttatgtccg agcgacgcca gcgcatttaa cagcatgagt
    20761 ggcttaactc cctggtaaaa gatcagcgtg ataatcgccg gcaggatagc ggaacggaac
    20821 aggcccgtca gctatcccgt ccagaaactg acgtacgcgc ggatcaggat tcgcctgcaa
    20881 cgcctgggca ctgccatgag cgacaatttt tttgtccgcc aggatccagg cgtgatccgc
    20941 aatacttaac acttccggca catcgtgaga aaccaccaca caagtcacgc ccagcgcgct
    21001 gttcagctca gaaatcagct tcaccagtac gcccatggtg atgggatctt gcccaacaaa
    21061 aggttcatca aacatgatga gatccggctc cagcgcaatc gcacgcgcca gcgctgcacg
    21121 ccgcgccatc ccaccggaaa gttcagaagg cattagttta gccgctccac gcagccccac
    21181 ggcctccagc ttcatcatca ccgtactatg caacaatggc gcgggtagtt gggtatgttc
    21241 gcgcagtgga taggcgacgt tgtcaaatac gttcatatca gtgaacaacg ccccggactg
    21301 aaataacatg ctcatccgtt tgcgcactgt atacaggcgc gaacgagaca tcgccggaat
    21361 attctcacca tcgaaaagga tctcaccatg atctggtgcg atttgcccgc caatcagacg
    21421 gagtagcgtc gttttaccga tgcccgatgg ccccatgatc gccgtgatct tccctcgcgg
    21481 cacggtcagg gaaatattat cgaagatgca gcgattgcca cgcgtaaaac tgacatcgcg
    21541 catatcgact aaattcgcca cagactgctc cataattcac ccttcgtctt gcgttgattt
    21601 tctaagcatg gcgctcaatt taaccttgaa cccaacatat ttacagaata ttacccgccg
    21661 tggttagcga aagctggcat ttgttttact ttttagccgc ataaagtcaa aattaagaat
    21721 ccgttacggc tttctgaaaa tcttcagcgg accgacgagt atacctgaag aaaggacgtt
    21781 agatgctttt agctacggca ctgttaattg ttggtttact tttggtcgtt tacagtgccg
    21841 accgcctggt ttttgccgcg tctattcttt gccgaacctt tggcatcccg ccgctgatca
    21901 tcggcatgac ggtggtcagt attggtacat cgttaccaga aatcatcgtc tcgcttgccg
    21961 cgtctctgca cgaacaacgc gatttagccg ttggtacagc cctcggctca aacattatca
    22021 atattttgct gatcctcggt ctggctgcgc tggttcgtcc ttttaccgtc cattccgatg
    22081 ttctgcgtcg tgaattaccc ttaatgttgt tggtcagcgt agtggccggt tccgtactct
    22141 atgacggaca acttagtcgc agcgatggta tctttctcct ctttctggct gtgctatggc
    22201 tgctgttcat tgttaaactt gcacgtcagg ctgaacgtca ggggactgac agcctgacca
    22261 gagagcagct tgcagagctg ccgcgtgacg gcggattgcc cgtcgcgttt ttatggctcg
    22321 gcattgcgct tatcatcatg ccagtggcca cgcggatggt ggttgataac gccacggtgc
    22381 tggcgaatta ctttgccatc agcgagttga cgatgggtct gacggcaatt gctatcggaa
    22441 ccagcctgcc ggaactggca accgcaatag cgggggttcg caaaggtgaa aacgacattg
    22501 ctgtcggaaa tatcattggc gcaaacattt ttaatattgt catcgtgttg ggtttacccg
    22561 cgctgataac gccaggagag attgagccat tggcgtacag tcgtgactac agcgtgatgt
    22621 tgctggtgag cattattttt gcgttgctgt gctggcggcg ctccccgcaa ccgggccgtg
    22681 gtgtaggggt attattaact ggcggattta tcgtatggct ggcgatgttg tactggttat
    22741 cgccaatact cgttgaataa ctggaaacgc attatgtcgc acgtagagtt acaaccgggt
    22801 tttgactttc agcaagcagg taaagaagtc ctggcgattg aacgtgaatg cctggcggag
    22861 cttgatcaat acatcaatca gaatttcacg cttgcctgtg aaaagatgtt ctggtgtaaa
    22921 gggaaagttg tcgtcatggg gatggggaaa tcggggcaca tcgggcgcaa aatggccgca
    22981 acgtttgcca gcaccggtac accttcattt ttcgtccatc ctggtgaagc cgcgcatggt
    23041 gatttaggca tggtcacccc acaggatgtg gtgattgcta tctctaactc aggtgaatcc
    23101 agcgaaatca cggccttaat tccagtgctt aagcgtcttc acgtaccgtt aatctgcatc
    23161 accggtcgcc cggagagcag catggcgcgc gccgcagatg tgcatctgtg tgttaaagta
    23221 gcgaaagaag cctgtccgtt agggctggca ccgaccagca gcaccaccgc cacgctggtt
    23281 atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg gctttactgc tgaagatttt
    23341 gcgctctcac acccaggcgg cgcactgggt cgtaaacttc tgctgcgcgt aaacgatatt
    23401 atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg ccagtctgcg tgacgcgttg
    23461 ctggaagtta cccgcaaaaa tcttggtatg actgtcattt gcgatgacaa tatgatgatt
    23521 gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg atatgggcgt ggatgttcgt
    23581 cagttaagta ttgccgatgt gatgacgccg gggggaatac gtgtgcgccc tggcattctg
    23641 gccgttgagg cactgaactt aatgcagtcc cgccatatca cctccgtgat ggttgccgat
    23701 ggcgaccatt tactcggagt gttacatatg catgatttac tgcgtgcagg cgtagtgtaa
    23761 agattcaagg ataaacaaca atgagcaaag caggtgcgtc gcttgcgacc tgttacggcc
    23821 ctgtcagcgc cgacgttata gcaaaagcag agaacattcg tctgctgatc ctcgatgtcg
    23881 atggcgtact gtcagatggc ctgatttata tgggcaataa tggcgaagag ctgaaagcgt
    23941 tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac ctctgatatt gaagtcgcta
    24001 tcattaccgg gcgaaaggct aaactggtag aagatcgttg tgccacattg gggatcactc
    24061 acttgtatca ggggcagtca aacaaactga tcgcctttag cgatctgctg gaaaaactgg
    24121 cgattgcccc ggaaaatgtg gcttatgtcg gcgatgatct catcgactgg ccggtaatgg
    24181 aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc actgttgatc ccgcgcgccg
    24241 attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg cgaagtttgc gacttattac
    24301 tcctggcgca gggcaaactg gatgaagcca aagggcaatc gatatgagta aagccagacg
    24361 ttgggttatc attgtgctat cactggcggt tctggtgatg atcggcattg atatggccga
    24421 aaaagacgat accgcccagg tggtcgtcaa caacaatgat cccacctata aaagcgagca
    24481 tacggacacg ctcgtctata acccagaagg ggcactaagc tatcgattga ttgctcaaca
    24541 cgttgaatat tattccgatc aggccgtttc gtggtttacg cagccggtac ttaccacgtt
    24601 tgataaggat aaaatcccga catggtccgt aaaagcagat aaagccaagc tgaccaatga
    24661 ccggatgctc tatttatatg gacacgttga agtcaacgca cttgtgccag actctcaact
    24721 tcgcagaatc acgacggata acgcgcagat caatctggtg acgcaggatg ttacctctga
    24781 agacctcgtc acgttatacg gaacaacatt taactccagc ggtctgaaaa tgcgcggcaa
    24841 cttacgcagc aagaacgccg agctgattga aaaggttaga acatcctatg aaattcaaaa
    24901 caaacaaact cagccttaat cttgtgcttg ccagctcact tctggccgcc agcattccgg
    24961 catttgccgt aaccggagac actgatcagc cgatccacat tgaatcggac cagcaatctc
    25021 ttgatatgca aggcaacgtg gttaccttta ccggtaatgt catcgtcacc cagggcacca
    25081 tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg cggcgaacaa ggtaaagaag
    25141 tgattgacgg ctacggtaaa ccggcaacgt tctaccagat gcaggacaac ggtaaacccg
    25201 ttgaaggtca cgcttcccag atgcactacg aactggcaaa agattttgtc gttctgacgg
    25261 gtaatgctta tctgcagcag gtcgatagca acattaaggg cgataagatc acttacctgg
    25321 taaaagagca gaaaatgcag gctttcagcg acaaaggcaa gcgcgtaacc accgttctgg
    25381 tgccgtcgca gctgcaggac aaaaacaaca aaggccagac cccggcacag aagaagggta
    25441 attaattcgt tatggcaaca ttaactgcaa agaaccttgc aaaagcctat aaaggccgtc
    25501 gcgtggtaga agacgtcagc ctgaccgtca actccgggga aattgtcggt ctgctggggc
    25561 caaacggtgc cggtaagacc accactttct acatggttgt aggcattgtg ccgcgcgatg
    25621 cgggcaacat cattattgat gatgacgata tcagtctgct gcctctgcat gcacgcgcgc
    25681 gccgcggtat cggctatctg ccacaggaag cctccatttt ccgtcgcctc agcgtttacg
    25741 ataacctgat ggcggtactg caaattcgtg acgacttgtc tgctgaacaa cgtgaagacc
    25801 gcgcgaacga gctgatggaa gagtttcaca ttgagcacct gcgtgacagc atggggcagt
    25861 cactctccgg gggtgaacgt cgccgtgtag aaattgcccg cgcactggct gcgaatccga
    25921 aatttattct gctcgacgaa ccgtttgccg gggttgaccc gatctcggtt atcgacatta
    25981 aacgcatcat tgagcacctg cgcgacagcg gcctgggcgt gctgatcact gaccacaacg
    26041 tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt cagtcagggg catttgatcg
    26101 cccacggcac gcctacagaa atcttacaag acgaacacgt taagcgtgta taccttgggg
    26161 aagacttcag actctgatag ggtagaagtt tgcgacgttt tagcaggaga gtacgattct
    26221 gaacatgaag caaggtttgc aactcaggct tagccaacaa ctggcgatga cgccacagct
    26281 ccaacaggca attcgtctgt tgcagttgtc gacgctggaa cttcagcagg agctacagca
    26341 ggcgctggag agtaatccgc tgcttgagca aatcgacact catgaagaaa tcgacacccg
    26401 cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg ctcgaacaaa aagagatgcc
    26461 ggaagagctg ccgctcgatg ccagttggga caccatttac accgctggta caccatccgg
    26521 caccagcggt gactacattg acgatgagct gccggtctat cagggcgaaa cgacgcagac
    26581 cttgcaggat tacctgatgt ggcaggtcga gctgacaccg ttttccgaca ctgaccgcgc
    26641 tattgctacc tctatcgtcg atgccgttga tgacatcggt tatctgactg tcccgctgga
    26701 agatattctc gaaagtatgg gcgatgaaga gatcgacatc gacgaggttg aagccgtcct
    26761 taagcggatc caacggtttg atccggtcgg tgtagcggca aaagatctgc gtgactgcct
    26821 gctaatccaa ctctcccaat tcgataaaac cacgccgtgg ctggaagagg ccagactgat
    26881 cattagcgat catctcgatc tgttagccaa tcacgacttc cgcactttaa tgcgcgtcac
    26941 gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg atccagtcgc tcgatccgcg
    27001 ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc attccagatg tgctggtgcg
    27061 taagcataac ggtcactgga cggtagaact caacagtgac agcattccgc gtctgcaaat
    27121 caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac gatggtgaca gccagtttat
    27181 ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt ctggaaagcc gtaacgatac
    27241 gctactgcgc gtgagtcgct gtatcgttga acagcagcaa gccttctttg agcaaggtga
    27301 agaatatatg aaaccgatgg tactggccga tatcgcccag gctgtcgaaa tgcatgaatc
    27361 gacgatatct cgcgtgacca cgcaaaaata cctgcatagt ccacgaggca tttttgaact
    27421 gaagtatttc ttttccagtc acatcaatac cgagggcggc ggcgaagctt cctccacggc
    27481 gattcgtgcg ctggtgaaga aattaatcgc ggcggaaaac ccagcgaaac cgttgagcga
    27541 cagcaagtta acctctttgc tgtcggaaca aggtatcatg gtggcacgcc gcactgttgc
    27601 gaagtaccga gagtctttat ccattccgcc gtcaaaccag cgtaaacagc tcgtttgacc
    27661 caaccgataa ggaagacact atgcagctca acattaccgg aaataacgtc gagatcaccg
    27721 aggcactgcg cgaatttgtt acagccaaat ttgccaaact tgagcaatat tttgaccgaa
    27781 tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac ccacacctca gatgcaacac
    27841 tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg tcaggacatg tacgctgcca
    27901 ttgatggctt aattgataag ctggcacgcc agttgaccaa acataaagat aaactgaaac
    27961 aacactaatt gtccgggcaa ttagcatgtg catggcggcc tgttgtgcgg cacaacgggc
    28021 catttgtacg gttaatgctc cgagcctgtt ccactgtttg agtgggcagg ttcttaggtg
    28081 aaattatgac aaataatgat acaactctac agcttagcag tgttcttaac agggaatgta
    28141 cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct ggaaatcatc agcgagctgg
    28201 cggcgaaaca acttagcctg ccgcctcagg tggtttttga agctatcctg acgcgggaaa
    28261 aaatgggcag taccggtatc ggcaatggta ttgccattcc gcatggcaaa ctggaagaag
    28321 atactctgcg cgccgttggc gttttcgttc agctcgaaac gcctatagct ttcgatgcca
    28381 tcgacaacca accggtggac ttactttttg ccctgctggt gtcggcagac caaactaaaa
    28441 cgcacctgca tactctgtcg ctggtggcga aacgtctggc ggacaaaacc atctgccgcc
    28501 gtttacgcgc agcccagagc gatgaagagc tgtatcaaat cattacggat accgaaggta
    28561 ctccggatga agcgtagtta ttcggtaatg tctcttttag acgttgtgag gagaaacagt
    28621 acatggtact gatgatcgtc agcggacgtt caggttcagg taaatctgtc gccctgcgtg
    28681 cgctggaaga tatgggtttt tactgcgtgg ataaccttcc cgtagtgttg ttacccgatc
    28741 tggctcgaac cctggccgat cgcgagattt ctgccgccgt cagcattgat gttcgtaata
    28801 tgccggagtc accagaaata ttcgaacagg cgatgagtaa cctgcctgac gctttctcac
    28861 cgcaactcct gttcctggat gccgaccgta ataccttaat tcgtcgttac agtgacacgc
    28921 gccgactgca tccgctttcc agcaaaaacc tgtcgctgga aagtgctatc gacaaagaaa
    28981 gcgatttgct ggagcctctg cgttcgcgag cggatctgat tgtcgatacc tcagaaatgt
    29041 ccgttcacga gctggctgaa atgctgcgta cccgtctgct gggtaaacgt gaacgcgaac
    29101 tgaccatggt ctttgagtct ttcggcttca aacacggtat ccctatcgat gcagattacg
    29161 tctttgacgt gcgcttcttg ccgaacccgc actgggatcc gaaactgcgt ccaatgacag
    29221 gtcttgataa acctgtcgcc gcgttcctcg accgccacac agaagtacac aattttatct
    29281 accagacgcg aagctatctt gagctatggt tacctatgct ggaaaccaac aaccgtagct
    29341 acttgacggt cgccattggt tgtaccggcg ggaagcaccg ttcggtgtat attgcagagc
    29401 aactggcaga ctacttccgc tcgcgcggta aaaacgtcca gtcacgccat cgtacgctgg
    29461 aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc acaaacaagc tgggcatgca
    29521 tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt tttgacgctg aagtgctgtt
    29581 acgtaatgac gaaggcaccg aggccgaagc caacagcgtt attgcgctgc tgatgttgga
    29641 ttctgccaaa ggacggcaga ttgaagttga agcgaccggc ccacaggaag aggaagcact
    29701 ggccgccgtt atcgccctct ttaactctgg ttttgatgaa gattaatctt catcactttg
    29761 acatacaact cccttcaaac tccccccgac aataagaaaa tcacgtactg aaatcgttct
    29821 caatcaacgt catttgtaca ttttgtgcgc ttttcacttt cagaagaacc ttaagaaaac
    29881 cttaagaggc attgtttagg ttttgtttaa gttaatcgac catactggag atcgtcagaa
    29941 aatatttcca ggagatggca tgattcgctt atctgaacaa agtcccctgg gcaccggaag
    30001 acatcggaaa tgttatgcgc atccggaaga tgcccaacgc tgtatcaaga ttgtctacca
    30061 tcgtggcgat ggtggcgata aagagatccg ccgtgagtta aagtactacg cgcatcttgg
    30121 tcgccggtta aaagactgga gtggaatacc gcgctatcac ggtaccgttg agacggattg
    30181 cggaaccggt tatgtctacg atgtgattgc agattttgac ggcaaactct ccattacgct
    30241 gaccgaattt gctgaacaat gccgttacga agaagacatt gcacaactgc gccagctact
    30301 gaaacagctg aagcgttatt tgcaggataa ccgtatcgtg acgatgtcgc tgaagccgca
    30361 gaatatcctc tgtcaccgca tcagtgagtc agaggttatc ccggtcgtct gcgacaacat
    30421 tggtgaaagc acattgattc cactggcgac ctggtcgaag tggtgttgtc tgcgtaagca
    30481 agagcggtta tggaaacgat ttatcgccca accggcgctg gctattgccc tgcagaaaga
    30541 tctgcaacct cgtgagagca aaacgctggc cctcacttcg cgcgaagctt aatccagctg
    30601 gtgttgttgc ataaacggct caccgcctaa ctgatacatc tgccgtaaaa tccacgcctg
    30661 acggctacgc acgtagcccg atggcgagga gactttgaaa cgaagtggat taggtaatac
    30721 agctgccagt aatgcagctt ccgaccgggt aagtttgctc gcgggtttgt ggaaataacg
    30781 ttgtgccgca gcttcgacgc caaacacacc gtcgccaaat tcggcgatat tcaggtaaac
    30841 cgtcaggata cgctttttgc tccagactgt ttctatcccc agcgttaatc cagcttccag
    30901 cccttttcgt acccagctac gcccatccca taaaaagaga tttttggccg tctgctgaga
    30961 aatcgttgaa gcaccgcgaa tacggttttc attgcgctcg ttgtgcgcca gggctttctc
    31021 aatggaagcg acatcaaagc cccagtgctc aggaaatttc tgatcttctg cggcaatcac
    31081 cgccagtccc atccacggcg agatttgatc catactgacc cagtcagaat gtgccacgta
    31141 acgaaaattg ccatgcagcc aggcgctgac ctgtcgctcg accattaccg ctgagaaggg
    31201 aacaggcgca acactaaaca acgcgatgcc cccgccccag aaaacggcga ggacaaccat
    31261 taacagcaaa agaaaacggc gaacgaaact aaacaccgtt aagcggcttt tactcattca
    31321 gccagaacca gcacgcggga aaccagctta tcaatgccgc tcgccgcttc tgcaatgttc
    31381 tgcgccagca tatatgctgg ggtggtgaca atcttattgt cttcatcaac cacgatatca
    31441 tcgacaggac agggcacatg ctccgcgccc atctcttcca gcacttctgc ggtatcgata
    31501 tcagtaccga tggtcaaacg cagcgggaaa tcgaaaattt tcggcagcat cgccggggca
    31561 atacacataa aaccaagcgg ttttccggcc tgatgcatcg cttgtgccag cgcctttaat
    31621 tcacggtcaa cggtgcattc gctaccaaga gtggcaaaat tgcttaaatt cttcgccgcg
    31681 ccaaaccccc ccggcacaat caacgcatcc agttcagcgg catcggcctg ggccagagga
    31741 cggatttcac cgcgcgttat tcgtgccgct tcaatcagca cgttgcgcgt ttccgtcatc
    31801 gcttcgccag ttaaatggtt gataacatca acctgctgct tatccggtgc aaagcagacc
    31861 gcctgcgcac cgctgcgtga aatagctaac aacgtcaaca ccgcttcatg aatttcagaa
    31921 ccgtcataga cgccgcatcc gctcagaatt acgccaattt tcttcattgt gatcatcctt
    31981 tcgcaattga ctgaaacaca ttaatttttt taataaaaat ggtacgcatc acacatttaa
    32041 ctgattcatg taacaaatca tttaagtttt gctatcttaa ctgcgtgcgg cctgaaaaac
    32101 agtgctgtgc ccttgtaact catcataata atttacggcg cagccaagat ttccctggtg
    32161 ttggcgcagt attcgcgcac cccggtctag ccggggtcat tttttagtgg cttttgccac
    32221 ccacgctttc agcacttcta cgtcgtgacg ccactcttct ttcatctctt caatccattc
    32281 accgacgtta tcttcccagg caggaaggtc aggagactga atttgctgac ccagctgttg
    32341 cagatggcgt aaccccactg accccgccgc acctttaatt ttatgtcctt cctcaacaat
    32401 gcctttttta tcctgcgccg tcagattcga ctccagcacg ctgacatagc ccggcatcat
    32461 cttctcaaac accgctaacc cgtcggtgat cagcttcggt cctacaagtt cgagatactg
    32521 ttccagcatg ggaatatcga gcaatgcttc tgatttactg ttctcttctg tcgtcaccgt
    32581 actctcctca tcatcctggg tatcccagaa tttcttgatc atcgcggtta gcgccggaac
    32641 agaaagcggc ttactcagca catcatccat tccagcattg aggtactctt gtttgtcttt
    32701 cagcacgtta gcggttaagg ccaccagcgg cggtaaatcc tctcgcggat aacgcttcgt
    32761 cagttcacga gagatatcca gcccggtcat atctggcaac tgaatatcca gtaacaccag
    32821 gtcgtattcg cccggtttaa acatctctag cgccgccttg ccggtcatgg cgacatcaac
    32881 gctgttacct aatttttcca gcacagaacg cgcaacaatc acgttcagtt caatgtcttc
    32941 caccagcagc acattcagcg ccggtaaagg catatcgtct tcatcaaacg catcatcgac
    33001 ctcttctgct accgacggtg cgtggatcgt caacgtaaag gttgaacctt tgccctgttc
    33061 gctggtaacc gtaatatcgc cgcccatatt tttcgccaga cgacgagaaa cggccagacc
    33121 aataccggtg ccggtggcag gtttaccgcc atgactgtct ttcacctggt aatacatggc
    33181 gaaaatttta tccagctcat cctgcggaat gccgatacca gagtcttcca cttcaaaatg
    33241 cagcatatcg ccttcatcgt agcgcacgcg cacggtaacc tggccttgct gggtgaattt
    33301 gacggcgtta ctgatgaggt tccacaggat ctgccgtaaa cgcgtcccgt cggtaatgac
    33361 ctgatgcggt aatggcagcg tcggctccag gttaaagcgc aatccttttt gttgcgcctg
    33421 caatgcggag agattttcca gatcggcaag gaagctggtg aaatcaaccg gttgattatc
    33481 aagctggacc ttgcgccgtt ccatcttatc catgtcgata atatcgttaa agatattccc
    33541 cagcgtgacg gccgaaacat ggatggtctt gagatatttt tcctgctcgg cggtgagttc
    33601 ggtatccagc agaatgcggc tcagaccgac gataccgttc agcggtgtac gcaattcgtg
    33661 actgatggtg gagataaacg tcgttttgtc gcggctggcc cgttcaagcg catcctgata
    33721 ccgcttacgc tcggtaatgt ctcgaccaaa gcccatcaaa ccgtgacgtt tacccacgcg
    33781 gtcgtagtac ggcactttac ggatttcaaa gcaggctttg cgcccgtccg ggtaatccag
    33841 ccactgttca taggtcagtg acacattatg acggaacact ttttcatcgg tttcaatgac
    33901 ttttgcggcg gcttccggtg agtaaacatc agcaggtttc aggtgaacca gttgtttttc
    33961 gctttttccg gtcagcagct ccatcgcgcg gttacagccg gaaaactctt tatcttcgtt
    34021 acgataaaaa accaggtcgg gtgaagcatc aaggaaggaa cgtaagaatg aggattgctg
    34081 ctcgagctga atttgtgtct cttcgcgctc tttgatttca attttcagtt ggccgaaggt
    34141 ttcctgcagt tctgcttccg ctttttcacg aacggcgatt tcctgattta gctgggcaat
    34201 attatctttt aactgaacgt tgaggctcaa atcgcgctcg cgcatctcct ccagtttttg
    34261 caccagccgt gacagacgtt gtcgtgactc ctccagttgc tcgacgacca ccgatagaaa
    34321 gtagaccgcc cacggcgtaa tcaacaaacc aaagaagata gaacgaataa catcaatgct
    34381 ttcgacctga ccatgcagca ccatggttac cgccatttgt accacaatgg caagaacgac
    34441 gagggccagc gccagcaaca ttgagaagcg caccagacct aacttcatca tcaggtcaac
    34501 ataatactgc gccagcagac gaatttgctt cattagggaa ttccttcacg acaacctgcc
    34561 ccaataatac ccaattctga cagttacgtt ataaattgtg tgagaaatgc ggaattcccc
    34621 tcaacgacct actccgttgg aggtagccac ggacgtccca gcgccgatcc ctgtacgcca
    34681 tgttcattca gatagcgatc cagctcgacc attcccgtcc atcgattttc gcaccacaga
    34741 ggagcaagca gcgtcgggcg acgggcgctg gcggaaatgc ggtggtagat cacttccggt
    34801 ggcgtatggc gaatcatttc tccggcagtg agcgtgtaat cctccagttc aataccgttc
    34861 aaacgccccg cttcccaggc tttcgccata atgctgcctt tcacaatatg caacggatgt
    34921 agctttatac cgtcaacacc ggtttccact acccgctcca gcgtttgcag acattcagcc
    34981 agcccttcgc ctggcagacc gacaattaaa tgcgagcaca ccttcagccc gcgctggcgt
    35041 gccagctgcg tcgtgcgctg ataacaggcg aaatcatgac cgcgattaat gcgatgtagc
    35101 gttttgtcgt gcacggtttg cagccccagc tccagccaca cttcgtagcc ctggtcttta
    35161 tattcgcaaa gcagatccag caccgcatcc ggcacgcagt ccgggcgcgt gccgacacac
    35221 aaaccgacaa tattggcctg gctcaccgcc tgttgataca tcgaacgcag cacctgaact
    35281 tcggcaaagg tgctggtata cgcctggaaa taggcgaggt aacgtttcgc gcgattaatt
    35341 aaattcgcct gatgcgccag ctgttcggca atggaacgat gctgctgcgc ttcgtcggca
    35401 aacgaggcaa cattacagaa ggtgcagccg ccgcgcccga tggtgccgtc gcgattcggg
    35461 caactgaaac cgccgtgcag cgtcagctta tgcacctttt gcccataacg acgggtgaga
    35521 tcaccaccaa acatattgac taatttctgt aactgcataa tctgatagac cgcgccttga
    35581 aaagaggcca aagcctgcca tttttagctc aattcggcga tgacctggat caatcgtccc
    35641 ggcccgcttt tatcaactgc ataatcaatc aaaattaccg aaatttcatg cataatcaca
    35701 taaatcactt ttgcttatct tgtgtcagat ttttttatct ccttatggat tttaggcaaa
    35761 aacagtagca tgaaacgtca ttaccaatta atgcagtata aaatgctggt tttctcgtca
    35821 attcaaggca ggataagggt taacacgcct ttatgacagt caggaattga ctgtttctct
    35881 aacgacttcc cttttagcct taaagataaa atctatttta atttcagcca tttaataaag
    35941 aattttgcgc taaagcacat ttctgtacca ataagcttgc catttgacct gtatcagctt
    36001 tcccgataag ttggaaatcc gctggaagct ttctggatga gcagcctgct catcatattt
    36061 atgcagtaat tgagatcccc tcttcaccgt attaaccgat gcgaaaagga caacaagggg
    36121 gcgaatgcga ggcgcgcgta tgacacgcaa cccccgtcgc cacgctcttt ctgtacccgt
    36181 gcgcagcggt tcggtagtgg ggttcccgca gagcctgggg gaggttcacg atatgttgta
    36241 cgataaatcc cttgagaggg ataactgtgg tttcggcctg atcgcccaca tagaaggcga
    36301 acctagccac aaggtagtgc gtactgcaat acacgcactg gcccgcatgc agcaccgtgg
    36361 cgcgattctc gccgatggta aaaccggtga cggttgcggc ttgctgttac aaaaaccgga
    36421 tcgctttttt cgcatcgttg cgcaggagcg cggctggcgt ttagcgaaaa actacgccgt
    36481 cgggatgcta ttcctgaata aagatcctga actcgccgct gccgcacgcc gcatcgttga
    36541 agaagaactg caacgcgaaa ccttgtcgat tgtgggctgg cgtgatgtcc ccactaacga
    36601 aggcgtactg ggtgaaatcg ccctctcctc tctgccccgc attgagcaaa tttttgtgaa
    36661 cgccccggca ggctggcgtc cgcgcgatat ggagcgccgt ctgtttatcg cccgccgccg
    36721 cattgaaaag cgtctcgaag ccgacaaaga cttctacgtc tgtagcctat cgaatctggt
    36781 gaacatctat aaaggtctgt gtatgccggc ggatctgccg cgcttttatc tggatcttgc
    36841 ggacctgcgt ctggaatcgg ccatttgcct gttccaccag cgcttctcca ctaacaccgt
    36901 accgcgctgg ccgctggcgc aaccgttccg ctatctggcg cataacggtg aaatcaacac
    36961 catcactggt aaccgccaat gggcgcgtgc gcgtacctat aaattccaga cgccgcttat
    37021 ccctgatctg cacgacgccg caccgttcgt caacgaaacc ggctctgact ccagttcgat
    37081 ggataacatg ctggaactcc tgctggcagg cgggatggat atcatccgcg ccatgcgtct
    37141 gttagtgcca cccgcctggc agaacaaccc ggatatggac ccggaactgc gtgccttctt
    37201 tgactttaac tccatgcata tggagccgtg ggatggcccg gcgggcatcg tgatgtccga
    37261 cggtcgtttt gccgcctgta acctcgaccg taacggtctg cgtccggcgc gctacgtcat
    37321 caccaaagat aagctcatca cctgcgcctc tgaagtcggt atctgggatt accagcctga
    37381 cgaagtggtc gaaaaaggcc gcgtcgggcc aggcgaactg atggttatcg acacccgcag
    37441 tgggcatatt ctgcactcgg cagaaaccga tgacgatctg aaaagccgcc atccatataa
    37501 agagtggatg gagaaaaacg tccgccgact ggtaccgttt gaagatctgc ccgatgaaga
    37561 agtgggtagc cgcgaactgg acgacgacac gcttgccagc taccagaaac agtttaacta
    37621 cagcgcggaa gagctggact ccgtaattcg cgtactgggc gaaaacggtc aggaagcggt
    37681 cggttcgatg ggcgatgata ccccattcgc cgtgctctcc agtcagccgc gcattattta
    37741 cgactacttc cgccagcagt ttgcccaggt gactaacccg ccaatcgacc cgctgcgtga
    37801 agcgcatgtt atgtcgctcg ccaccagtat cggtcgtgaa atgaacgtct tttgcgaagc
    37861 agagggccag gcgcaccgtt taagctttaa atcgccgatt ctgctctact ccgatttcaa
    37921 acagctcacg acgatgaaag aggagcacta ccgcgcagat acgctggata tcacctttga
    37981 cgtcactaaa accacgctcg aagcgacagt caaagagctg tgcgacaaag ccgaaaaaat
    38041 ggtacgtagc ggcaccgtgc tgctggtgct ctccgaccgg aatatcgcta aagatcgcct
    38101 gccggttcca gccccgatgg cggttggcgc gatccagacc cgtctggtcg atcaaagcct
    38161 gcgttgcgat gccaacatca tcgtcgaaac cgccagcgcc cgcgatccgc accacttcgc
    38221 cgtgttgctg ggcttcggcg cgacggctat ttatccatac cttgcctatg aaacgctggg
    38281 ccgcctggta gacacccatg cgattgccaa agattatcgt accgtgatgc tcaactaccg
    38341 taacggcatc aacaaaggct tgtacaaaat catgtccaaa atgggcatct ccaccatcgc
    38401 ctcttaccgc tgctcgaaac tgtttgaagc ggtcggtcta cacgatgatg tagtgggcct
    38461 gtgcttccag ggggcggtca gccgcattgg tggggcaagc tttgaagact tccagcagga
    38521 tctgctgaat ctgtcgaaac gtgcctggct ggcgcgtaag cccatcagcc agggcggcct
    38581 gctgaaatac gtccacggcg gcgaatacca cgcctacaac ccggacgtgg tgcgcacgct
    38641 gcaacaagcg gtacaaagcg gcgagtacag cgactatcag gaatacgcga agctggttaa
    38701 tgagcgtccg gcaaccacgc tgcgcgatct gctggcaatt acgccgggtg aaaacgcggt
    38761 caacattgct gatgttgaac cggcaagcga actgtttaaa cgcttcgata ccgctgcgat
    38821 gtctatcggc gcgttaagcc cggaagccca cgaggcgctg gcggaagcga tgaacagcat
    38881 cggcggtaac tcaaactccg gtgaaggcgg tgaagatccg gcgcgctacg gcaccaacaa
    38941 agtgtcgcgc atcaagcagg tggcttccgg tcgctttggg gttactccgg cgtatctggt
    39001 caatgccgac gtcattcaga ttaaagtcgc ccagggcgcg aagccgggcg aaggcggtca
    39061 gttgccgggt gataaagtca ctccttacat cgccaaactg cgctattcgg tgcccggagt
    39121 gacgctgatc tccccgccgc cgcaccacga tatctactct atcgaggact tagcgcagct
    39181 cattttcgac ctcaagcagg ttaacccgaa agcgatgatc tccgtgaagc tggtttccga
    39241 accgggcgta ggcaccatcg cgactggcgt ggcaaaagct tatgccgatt taatcaccat
    39301 cgcaggctat gacggtggca ccggcgcaag cccgctgtcg tcagtgaaat acgcaggttg
    39361 cccgtgggag ctggggcttg ttgaaaccca gcaggcgctg gttgccaatg gtctgcgtca
    39421 taagatccgt ttgcaggtcg atggcggcct gaaaaccggt gttgatatca tcaaagcggc
    39481 gattctcggc gcagaaagct tcggcttcgg cactggccca atggtcgcgc tcggctgtaa
    39541 atatctgcgt atttgccacc tgaacaactg cgcaacgggt gtggcaactc aggatgacaa
    39601 actgcgtaag aatcactatc acggcctgcc gttcaaggtg acgaattact ttgagtttat
    39661 cgcccgtgaa acccgcgagc tgatggcaca gctgggggta acgcggctgg tggatctgat
    39721 tggtcgcacc gacctgctga aagagctgga cggtttcacc gccaaacagc agaaactggc
    39781 gctgtcgaag ctgctggaga ccgccgaacc gcatccaggt aaggcactct actgcaccga
    39841 aaacaacccg ccgtttgata acggcctgct gaacgcgcag ttgctgcaac aggcgaaacc
    39901 gtttgtcgat gaacgccaga gcaaaacctt ctggttcgat atccgcaata ccgatcgctc
    39961 cgtgggcgcg tcgctttcag gctatatcgc ccagacgcac ggcgatcagg ggctggcagc
    40021 cgatcctatc aaagcgtact tcaacggcac cgcaggccag agtttcggtg tatggaacgc
    40081 gggcggcgtg gaactgtacc tgaccgggga tgccaacgac tatgtcggta aaggcatggc
    40141 gggcggctta atcgccattc gtcctccggt tggttccgcc ttccgcagcc atgaagcaag
    40201 tattatcggc aacacctgcc tgtatggcgc gaccggtggt cgtctgtatg ccgcaggccg
    40261 cgcgggtgaa cgtttcggcg tgcggaactc cggtgctatc accgtggtag aaggcattgg
    40321 cgacaacggc tgtgaatata tgacgggcgg tattgtctgt attctgggta aaaccggcgt
    40381 taacttcggt gcgggcatga ccggcggctt cgcttacgtt ctcgatgaaa gcggtgattt
    40441 ccgcaaacgc gttaacccgg aactggtcga ggtcttaagc gttgacgatc tggcgatcca
    40501 tgaagagcat ctgcgcggtc ttatcaccga gcatgttcag catactggct ctcagcgcgg
    40561 tgaagagatt ctggcgaact ggtcaacctt cgccactaaa tttgcgctgg ttaaaccgaa
    40621 gtccagtgat gtaaaagcac tgctgggtca tcgtagtcgt agcgcagcag agttgcgcgt
    40681 gcaggcgcag taaggggtag caacaatgag tcagaatgtt tatcaattta tcgacctgca
    40741 gcgcgttgat ccgccaaaga aaccgctgaa gatccgcaaa attgagtttg ttgaaattta
    40801 cgagccgttt tccgaaggcc aggccaaagc gcaggctgac cgctgcctgt cgtgcggcaa
    40861 cccatactgc gagtggaaat gtccggtaca caactacatc ccgaactggc tgaagctcgc
    40921 caacgagggg cgtatttttg aagcggcgga actgtcgcac cagaccaaca ccctgccgga
    40981 agtttgcgga cgagtctgcc cgcaagaccg tctgtgcgaa ggttcctgca ctctgaacga
    41041 tgagtttggc gcggtgacca tcggcaacat tgagcgttat atcaatgata aagcgttcga
    41101 aatgggctgg cgtccggata tgtccggcgt gaagcaaacc ggtaaaaaag tggcgattat
    41161 cggcgcaggc ccggcaggtc tggcgtgtgc ggatgtcctg acgcgtaacg gcgtaaaagc
    41221 cgttgtcttc gaccgtcatc cagaaattgg cgggctgctg accttcggta ttccggcctt
    41281 caagctggaa aaagaggtaa tgacgcgtcg ccgtgaaatc ttcaccggca tgggtattga
    41341 attcaaactc aataccgaag tgggccgcga cgtgcagctg gacgatctgc tgagtgatta
    41401 cgatgccgtc ttccttggcg tcgggactta tcagtcaatg cgcggcgggc tggaaaacga
    41461 agacgccgat ggcgtgtacg cagcgctgcc gttccttatc gccaacacca aacagttaat
    41521 gggctttggt gaaacccgcg acgaaccgtt cgtcagcatg gaaggcaaac gcgtggtggt
    41581 ccttggcggt ggcgacactg cgatggactg cgtgcgtacg tccgtgcgcc agggagcgaa
    41641 gcacgttacc tgtgcctatc gtcgtgatga agagaacatg ccgggttccc gccgcgaagt
    41701 gaaaaacgcg cgggaagaag gcgtagagtt caaattcaac gtccagccac tggggattga
    41761 agtgaacggt aacggcaaag tcagcggcgt aaaaatggta cgtaccgaaa tgggcgaacc
    41821 ggatgccaaa ggccgtcgcc gcgcggagat cgttgcaggt tccgaacata tcgttccggc
    41881 agatgcagtg atcatggcgt ttggtttccg tccacacaac atggaatggc tggcaaaaca
    41941 cagcgtcgag ctggattcac aaggccgcat catcgccccg gaaggcagcg acaacgcctt
    42001 ccagaccagc aacccgaaaa tctttgctgg cggcgatatc gtccgtggtt ccgatctggt
    42061 ggtaaccgct attgccgaag gtcgtaaggc ggcagacggt attatgaact ggctggaagt
    42121 ttaagctctg tcccagcgcc gcctcctcag aaaagagtcg gcgctgaaat tctcctttct
    42181 ttctggaaag cgcctcgcat aatctgaaga aaaccaatgt aatacgctca tctggattgt
    42241 taccggtaga tgcccggcgt atagctgatt ccattattca acgcagcgcc atataaaggg
    42301 acagataatg gaatcgctct cagagagaac ctcaacaggc taccagcaaa tccacgacgg
    42361 tattattcac ctggtcgata gcgcccggac ggaaacggta cgtagcgtta acgcgctgat
    42421 gaccgcgaca tactgggaaa ttggccgacg aattgtcgaa tttgaacaag gtggcgaggc
    42481 aagggctgcg tatggtgcgc agctaatcaa gcgactatca aaggatttaa gtctaaggta
    42541 taagcgtggt ttctctgcaa aaaacttacg ccaaatgagg cttttttacc tcttttttca
    42601 acatgttgaa attcgccaga cagtgtctgg cgaattaaca ccattgggaa ttccgcagac
    42661 accatctgca gaattcccat cggcaaagat ttggcagacg ctgtctgcca aattgagagg
    42721 ctgtttgata tttccacagc ttttgtattg catctcctat tttcggtttg tatattcatg
    42781 caaataaacg aaggaaagtt gccgtgattt acattgaaca gcttgaatta atccataaaa
    42841 gtggtgatgt gttgtatcca gtaaaaataa ccagaaaatc atcaggaaaa actgccttcc
    42901 accttgttcc attcggctta aataagactc acgaccttct agaggtggaa gatgcttcag
    42961 aagcaattcg gctggtgatt gatgaacgtc attccattcg ctgcagtaca cttacagcaa
    43021 ctattacgaa taaaaagggc aagcgaatta agcggacagg tatatacagt atcaagggtg
    43081 ttaacataaa agaatacaac gtcaggtaat ggcacagagc ggtccgtcag ttcaggtttg
    43141 gctctgtgcc aaagatgggt caggtcacac ctgatctaat aaatttaagc cattaaatca
    43201 gccttaacct tcaccaccac ctttttaatc tcacccggct cgccaacaac gcatcccggc
    43261 ttatgcggct ctcctagcat aaacacggcg aacattcccg gtttcaggat gatggcttgc
    43321 tcgttttcaa tggtgctgca aagctggtaa tcatcctcat ggtggaactc ttcacactga
    43381 cgcgcagtgc ctgccatgcc aaacagaatc cgttcttcac cgtttaataa cagctggata
    43441 tcaatgtatt gctcgtgcag ctccgctttt ttctcaacgg gcgattgagt gttaaacgtc
    43501 atgacattca taaaaatatt gtcgccctgt aattcgtaac gacccggcgc tttttcttgc
    43561 ggtctggcag ctaatgccag cgttaacgcg tcctgtaacg caggatgtaa cccagcagac
    43621 ggtaatgacc gtacttcacc catcatcata atttttctcc ctgggccaac agcgctgccc
    43681 caagtaaacc tgcatcatgg cggtagtgcg ccgccagtaa atcaacatga aataccgctg
    43741 gctcctgcgc cagatacgtt tccaccaacg ccagataccc ttctgccaga ccaacgctgc
    43801 caccgaccac cacgcactgg caatcagttg tggctttaat atcagcgatc agccttgcca
    43861 gcacatgtgc ggagcggtga atcagttgct gcgcctgctc gtcgccctgc ccggcgcgtg
    43921 tgaaaatagt tctcgcatcc gcgcccatga gttcaccttg cgctgccgct gcaataccgc
    43981 gaccagaagc aatcgcttcc acgcaacctg tgcgtccaca gccgcagact gggccgtgtg
    44041 gatcggcaag cgtatgcccg atatgccccg ccagaccgcc agggccggta agcagtttac
    44101 cgccgctcac tacaccgccg ccgacgccag tggaaacggt gataaacacc atctcggtta
    44161 catcgccttc cagcgcctga tactctgccc atgctgcggc ctgcgcgtcg ttaatggcaa
    44221 tggtcggcaa attggtaagt tgttccagcg ttttgactaa cggaaagtgt agcaatccac
    44281 cgagattatg cggattcagc gccagcaaac tgccgtcgcg aataattccg gttgaagcga
    44341 tggcaacccg ctgcgcatga gcttgcaacg gagagactaa tgcggataag gcatcacgca
    44401 aggcttctgg tgtctggctg gctggcgtag gaagttcacg acgatcgcgg atctgcccgt
    44461 cagcgccaat cagcgcggcg gcaagtttag taccgccgat atcaatcgcc agtgtggtca
    44521 tagcaccgcc tttttcatcg ctgtgttgta ccactgacaa atgtgttcaa gacgcgtaat
    44581 ggcagaaccg accgtcaccg cccacgcgcc gtggcgcatc gcatctgccg cctgagcagg
    44641 cgtgttgtaa cgcccttcgg caatcacccg acatccggct tcgctcaacg ttttcaccag
    44701 cgccagatcc ggctcttctg gcgtttcagg cgtggtatag ccagaaagcg tagtgccaat
    44761 aatttcggcg cccagctttt ggcatgccag gccgtcttcc ggcgttgagc agtcggtcat
    44821 cgccagtaaa ccgtgatgat gtatacgtgc cagcagcgtt tcaacaggca ccggacgcgg
    44881 gcggtcggtg ccgtcaatgg cgataatgtc cgcgcccgcc tgcgccagcg catcgacatc
    44941 ttcaatatag gccgtgatgc gtaccggaga atcctccaga tcgcgtttca caattccaat
    45001 aatcggcacg ctcaccaccg cacgcgtggc ttgcagattt gccacacctt cgatgcgaat
    45061 ggcaaccgcg cccgcctgct ctgccgctaa tgccatggct gcgacgattt cgggtttatc
    45121 gagcgggctg tcaggaatcg gctggcagga gacaatcagg ccaccgttag cagcgatttt
    45181 ttgatccagt tgtgcaagta acgacataca tcttccctta gcgaaaggcc cggtacatag
    45241 accgggcaac aggattaact tttggttttg actaaatcgt ttttggcgct gccaaatggc
    45301 acggcaccgc taaatggttt gccgtcgata gcgtcatgag tacgcaatgc ttccgggcgc
    45361 agccaacgct gaacgcggga aggcatatcc agaccaatca gcaggatcac cacaaatgtc
    45421 aggctgaacg agagcgatgc cagcgcagta cccagatcca gacgttgagc gatcaacgcg
    45481 ccgatgattg gggccagtgc gccacccaat gcgccaacgt tgtaggtaaa gcccaggccc
    45541 gctgcacgct ggtcggtatc gaaataaccg ccaatcagtt tcggtaagat cccggcgatc
    45601 ccttgtccaa gcatttgctg gaagaacagt aacagaccga gcacccagac gtttgcgccg
    45661 ccaatcgcaa ataccggaat aatcagcagc tgcgaggcca gcaggctaca aacgtacgct
    45721 ttgcgggttc ccagccagtc accgaggaag cctcctacgc agcatcccac ggccgcgcca
    45781 aagccactaa agaacagcac attggcgaca gtatgcgggt tataagccag atcggttttc
    45841 agatacgttg gcagcagcgc ctgaatcggc catgagtaga ggaaagcaaa caacacgacc
    45901 accatcagca ttacgcccgt tggccagcgt ttgcctgtac tctgcaccat aaagctgata
    45961 aagattgcgg cgcataacag cccaagaaca gcgacgatcg cggcattttg caggttaccg
    46021 gcgaagcaga accacagcgc agtagccgcc gccagtgtca ttacgatatt ggcaatgcga
    46081 tgttcaccac ggtagagaat atccaccatt gtgcgtactg gtgctttacc tgcgtgtttc
    46141 tctttccagt cttccgcttc cgggatgttt ttacgcagcc agagagcaaa gatgattggc
    46201 aaaatgccga taaagaacag cgcacgccaa ccccagaccg gaaccaccag gctatagacc
    46261 tgagcggcaa cgacggcccc cacagagaag cctgaaatca aaaaaccact ggctttgtta
    46321 cgcagatgtt ttggccagct ttcaatgaca taggtggcgc tggaaccgta ttcacccgcc
    46381 atccccatgc cgatgaccag acgagcgata aacatggtga tgtagcctgg cgcaaagccg
    46441 caggccagcg tcccggccga gaagagaacg atgctggtga ccattgccag acgacgcccg
    46501 tagcggtcac ccatagcgcc gagcatcagg ccgccgaacc agcgagagat aaaggctgcg
    46561 gagatcagac ttgccgcctg caccgtcgtc agcccgaatt caccctgtac ttcggtgagt
    46621 accagggcga ttaaaacgaa atcaaaaccg tcaagcagat atcccaacca ggcagcggaa
    46681 aatgcgcgcc attgtgcacg gttgagatgg cgataccacg ggatattctg ggttgtagta
    46741 ctcattgtga gtctcccgcg gtgggcgatg cccacacgct ttggtatgaa aattgtaggg
    46801 tacagatgcg tttatttccc ctcacccggt aggggcgagc gaggggaaaa caactcaccc
    46861 gcgctcttgc atcaactgct gggccagcgc cttcagttct ggcagatatt tttcatctac
    46921 cggtccaaac ggtttgcggc acagcggcac agaaacgaca tccatataat gcaggacagt
    46981 tttcaggccg cggaatacgc cagttttgat cagtaaatca atgactttgt tgcactcagt
    47041 ttgcagtttc tgcgcggtct ggatatcgcc ttctttcagc gccttcacga tcccctgata
    47101 tcgccagccc atgatgttgt aagtactgcc gataccacca tcagcgcctg ccagcaagcc
    47161 ggaagcgaag atttcgtcgt aaccgttata gagcaccaga tcaggatgtt cacgacggat
    47221 ctgttccatc tgatagagat cgccagaagt ctgtttcagc gcaccgacgc caggcaacgt
    47281 aaccagggtg ttgatctgat ccagggtcag ttttacccca ctcagggctg gaatgttgta
    47341 cacaaccatc ggcaaaccat ccgctgaatc aataattgcc cgatagtgat cgcagtgttc
    47401 ttcaaagctg aaaggatagt agaacggcgt gacggcggag acggcatcga agccataacg
    47461 tttagccgat gccgcaagtt gttggctttc ggcggtgctg acgcaaccga cgtgggcgat
    47521 gagtttaatc ttacctttcg cctcttcggc gacgatttcc agtacctgtt cacgctcgga
    47581 aaggctttgt acaaaggcct cgccggtcga accacccacg tataaaccgt cgatgccctg
    47641 ctgaatattg aactgaacca ggcgacgcag actcgcttta tccagtgctt gttgttggtc
    47701 aaaaggagtc aggagtgcag ccattacgcc acgtaaattc gttgccataa atacctctga
    47761 agtgatactt gtctgataaa cgatatacct ttatacctgt tataccagat caattaagca
    47821 acaccccata cagaaagctt ataatgcgat ctgcttcact aaagtggcat tatttctttt
    47881 tgttggtggt ctgaccgaaa gcgtgccagg tagcagagac gctgttgaga tgcgattgca
    47941 acgcacgatc ggcttcgtca ggatcatgac ggcggatcgc atcaacgatc gcaatatgct
    48001 gttgatagct aacgttgtta tgctcgtgca gagcctgatc agccacggtt ggacgtgcgg
    48061 cgatcagcca gtcgagcagg gcaacgtgga tcgccatgaa gattgggtta ccagggatct
    48121 ccgccagcac gcggtggaaa tcaacgtctg aacgaatgaa tgccgcgttg ttatccagcg
    48181 actgactgtt gatttccagt gcttttgcca gcaaatcgat ttgctcatcg gtggcatgtt
    48241 cagccgcata gcgcaccaga ctggattcaa agaacagacg taattgttcg aaatgggcaa
    48301 tcccaccggg atgagaaagg aaatctttcg ccatgccgga aaactcaccg atgatagtgt
    48361 ccgcagaagg acgcgagacg cgagcgcgtt cgccgttgtt tatttgcacc agacctttgc
    48421 gttttaacgc tgccagcgct tcgcgcacag aaggacgccc gacgttaaag aacgccatca
    48481 gttcgcgttc agacggtaat tgttcacctt cgccaaattc acgacggcgg atcatctgtt
    48541 ccagctcttc ttccaccatt tcggagagtt ttttacgcgc cagcgggcgg ctacgcaagt
    48601 tgcgaccaat tgcaggtgaa gaatcttcgg tttgcgaatc aaatgcgttc ataaggccca
    48661 ttctgtaagg tcagtgtgat taacatcatc agtgacatcc tatcacagga ttgaaagtag
    48721 gggaaaatgg caggtttttc tctttgtgcc tcatcattac cataattaac ggaataatta
    48781 actattgcga aaaattaatg taacgcagat aaacacatcc cgattgaatt attcataaga
    48841 ctattcacaa gcattatgaa tattatgaat gtgttcttac aaaataatca taagcgcata
    48901 ttttttaatg aaaaatcaca tcacctacaa ttaaaaacac gacatccgca ccataaatag
    48961 acttgcaaaa aatataacat cattgttttc aatctgccgt ttatgggatt gaccgttttc
    49021 ttttgacacg gagttcaaca atgttcggca taattatatc tgtcatcgta ttaattacga
    49081 tgggctattt gatcctgaaa aactacaaac ctcaggtggt gctggctgcc gcaggtgtct
    49141 tcctgatgat gtgcggagtc tggttagggt tcggtggtgt actcgatccc gccaaaagca
    49201 gcggctactt gatcgtcgac atttataatg aaatcctgcg catgctgtcc aaccgcattg
    49261 ccggattggg gctgtcgatt atggcggtgg gcggttatgc ccgctacatg gagcgcatag
    49321 gagccagtcg cgcgatggtg agcttgctaa gccgcccgtt aaaactcatt cgctcgccgt
    49381 atattattct gtcggcaact tacgtcatcg gccaaatcat ggcgcagttt attaccagcg
    49441 cctccggtct gggtatgttg ctgatggtca ccttatttcc gacgctggtg agtctgggag
    49501 taagtcgcct ctctgcggtg gcggttatcg caaccacgat gtccattgag tgggggattc
    49561 tggaaacgaa ctccattttt gcagcccagg tcgcgggaat gaaaattgcc acatacttct
    49621 tccactacca gcttccggtc gcctcttgcg tcattatctc ggtggcgatc tcccactttt
    49681 tcgtgcaacg cgcttttgac aaaaaagata aaaatatcaa tcacgaacag gcagagcaaa
    49741 aaactctcga taatgtcccg ccgctctatt acgccatttt acctgtgatg ccgttaatcc
    49801 tgatgctcgg ctcgctgttc ctcgcccacg tcgggctgat gaagtcagaa ctgcatctgg
    49861 tggtggtgat gttactaagt ttgacggtga cgatgtttgt tgagttcttc cgcaagcata
    49921 acttgcgcga aacaatggac gatgtgcagg cgttttttga cggcatgggt acgcagtttg
    49981 ccaacgtggt aacgctggtg gtcgcgggtg aaatatttgc gaaaggctta acgacgattg
    50041 gcactgttga tgcggttatc aggggtgcgg agcattctgg tctgggcggt attggcgtga
    50101 tgattattat ggcgctagtc attgccattt gtgccattgt gatgggctct ggcaatgcgc
    50161 cgtttatgtc atttgccagt cttattccga atatcgcagc cggactacat gtaccagcgg
    50221 ttgtaatgat tatgccgatg cattttgcca cgacgctagc gcgcgcggtt tcgccgatta
    50281 ctgcggtggt gaagcggaca gcgatcccca tggcagtcgg tttcgtggtg aatatgattg
    50341 ccacaatcac gctattttat taagtcatta aaaagacaaa acaggccgcc tgggcctgtt
    50401 ttgtattact tcacaacacg taatgccggt cgaccaccgc gtggtggctg cggaggttca
    50461 tcgtcaggat gagtgtcatc atcgtgatct ggcttgtcgc catcaataac cgacataacg
    50521 gtttcgttgt ctgccgatgc ctcttcatca ttcatgatgc tggtatcttc atcgtaggca
    50581 gcttcaggct caaacatcgt gcctgcgcca ttttcacggg cgtagatagc cagcacggca
    50641 gccagcggca cagaaacctg acgcggaatg ccaccaaagc gcgcgttaaa gcgcacctca
    50701 tcattcgcca gttccagatt gccgacagca cgcggcgcaa tgttgagtac gatttgcccg
    50761 tcacgcgcat attccatagg aacctgcacg ccagggagcg tcacatccac caccaggtgc
    50821 ggcgtgagct ggttatccag caaccactca tagaatgcac gcagcagata gggacgacgt
    50881 ggtgttagct gtgacaaatc catacagatt aactccggcc cagacgcatt tcacgttctg
    50941 cttcagttaa agaagcaagg aaagagtcac gctcaaagac gcgggtcata tagcctttca
    51001 gctctttcgc acccgggccg ctgagctcga tacccagttg cggcagacgc cacagcagcg
    51061 gagcaagata gcaatcgacc aggctgaact catcgctcag gaagtacggc ttctgaccga
    51121 agaccggcgc aatcgccagc agttcttcgc gcagttgctt acgtgcggca tctgcttcag
    51181 aagctgaacc gttgatgatg gtgttcatca gcgtgtacca gtctttttcg atgcgatgca
    51241 tgtacagacg gctttcaccg cgagctaccg ggtaaacagg catcagtggc ggatgcggga
    51301 aacgctcatc cagatattcc ataatgatgc gagattccca cagggtcagc tcacgatcca
    51361 ccagggtcgg aacgctctga ttcgggttga ggtcaatcag atcctgaggc ggattgtcct
    51421 tttccacgtg ttcgatctcg aaacttacac ctttctcagc cagcacaatg cggacctgat
    51481 ggctatagat gtcagtagga ccggaaaaca gcgtcattac cgaacgtttg ttggcagcga
    51541 cagccatgaa aacctccagg tatagtcaga atttttactg ctaccagcca ccaggtggcc
    51601 agtcagaagt tgtgttaccc aataaggaac gactctcttt gttcgaaaat caaacaaaaa
    51661 atgagcaata cccgacattt gggcagaaaa ttggatgata gtttaccaga ttttgcgacc
    51721 attgtggtga gtggatgccg gaaatgggga aaaagagatg cgctttagtc tgaaatagtt
    51781 gacttagacc cttattggcg atgtggtttt tgttttgcct gtctttcaga cggcagcaaa
    51841 aagcaacttc ccatttttta cgctgattca gaatttagcc ataaaaaaac ccgccgaagc
    51901 gggttttttc gtaaattgtt ttctgccgga gcagaagcca attaacgttt ggagaactgc
    51961 ggacgacgac gtgctttacg cagaccgact ttcttacgtt caacctgacg agcgtcacga
    52021 gtaacgaagc cagctttacg cagttcagaa cgcagggact cgtcgtattc catcagagcg
    52081 cgggtgatac cgtgacggat cgcaccagcc tgaccagaga taccaccacc tttaacggtg
    52141 atgtacaggt ccagtttctc aaccatgtcg accagttcca gcggctgacg aactaccatg
    52201 cgggcagttt cacgaccgaa gtactgttcc agagaacgtt ggttgattac gattttaccg
    52261 ttgcccggtt tgatgaaaac gcgagctgcg gaatttttgc ggcgaccagt gccgtagtat
    52321 tgattttcag ccattgccta taatcccgat tagatgtcaa gaacttgcgg ttgctgtgcc
    52381 gcgtggttgt gctcgttacc cgcgtaaact ttcagtttac ggaacatagc acgacccagc
    52441 gggccttttg gcaacatgcc tttaaccgcg atttcaatca cacgctcagg acggcgagca
    52501 atcatctctt caaagctcac ttgtttgata ccaccgatgt ggccggtgtg gtgatagtac
    52561 actttgtcag tacgcttgtt gccggttaca gcaactttgt cagcgttcag aacgatgatg
    52621 taatcaccgg tatctacgtg cggagtgtat tccgctttgt gcttaccgcg taggcgacga
    52681 gccagttcag tagccagacg gcccagagtt ttaccggtcg cgtcaacaac ataccagtcg
    52741 cgttttacgg tttctggttt agctgtaaaa gttttcatta aaagcttacc caataaatag
    52801 ttacacgttg gtgaacaccc aaacgtcttc aattgttgag gttcacacga caaagtccgg
    52861 caaacctacc ccttcgaata gcctatgcca gcacacaaaa agttttggga aaaaaacttt
    52921 cttgtaacgt ggggtcgcag gattatagag aagtcggggt caaagatcga cccctttttg
    52981 tgatttgtga caggttttaa cccgccaaat gctcgcgctt cagatactct tcgctttgca
    53041 tctcttgcag acgtgacagg caacgctgga actcaaactt cagccgatcg ccctgataaa
    53101 tttcatacag cggcacttct gcactcacca ctaatttgac atggcgctcg taaaactcat
    53161 ccaccagcgc aataaagcgc cgcgcttcgc tctccatcaa ccgcgtcata actggtacat
    53221 caaacaacat gaccgtatga aagagacgtg agagcgcaat atagtcatgc tgactgcggg
    53281 cgtcgacgca cagcgtagta aaagagaccg ccagcgtctg gttctcgacg cccattgtcg
    53341 ctaatggccg atggttgatt tctaacgtcg gtgaattttc tcgtttcccc cccgccagcg
    53401 ccaaccatag tttatccatt tgcgcccggg tttcatcgtg gagtggcgaa agccacagat
    53461 gcgcctgagt gagtgtacgc agtcgataat caacaccagc gtccacgttc attacatcac
    53521 aatgctgttt aatggcatcg attgcaggca gaaaacgcgc acgttgcaag ccatttcgat
    53581 aaagttcgtc cggcggaata tttgacgtcg ctaccagggt aataccgcgg gcgaacaagg
    53641 ctttcatcag accgccaagt agcatggcat cggtaatatc agaaacaaaa aattcgtcaa
    53701 aacagagcac gtcagtttcg gctttaaagc gatcggcaat aatttccagc ggatcggtct
    53761 gcccctgtaa ggcagttagc tcttcatgca cccgcagcat aaaacggtga aagtgcaggc
    53821 gctgtttccg ctctcccggc aggctttgat agaaaaggtc catcagccag gttttcccgc
    53881 gtcctacacc gccccacata tataagccac gcactggcgt atgctttgtg tcttcgcgtt
    53941 taccccacag cttaccgacc cgcgccatta gcccactcgt cctgggggct ggtggcgtgc
    54001 tattgatgag ttcctgataa ataatttcca ggcggctgac ggcctctttt tgaacgtcgt
    54061 cgggttgatg gctgccttca ttgagcgcct tcaggtattg cgatgttggg gtaacgcttt
    54121 gcatgatctt attgttattc cttgaataat cggtgcgccg ttgttcacgg ttgacgaaaa
    54181 aaaggccgtt ctacactacg cgatatgcag tcgggattcc acttctgtgg aattaacggt
    54241 tatagtggca taatcagccg caggcatgga gcctgaagcc aacaccctac ggaaacaaaa
    54301 gacaacggga gatgttcatg acctgggaat atgcgctaat tgggttagtc gtcggcatca
    54361 ttattggtgc tgtggccatg cgttttggta atcgtaaact acgccagcaa caggcgttgc
    54421 agtacgaact ggaaaagaat aaagctgaac tggacgagta tcgcgaagag ctggttagcc
    54481 actttgcccg cagcgcggaa ttactggata ccatggcgca cgactatcgc cagctgtatc
    54541 agcacatggc gaaaagctcc agcagcctgc tgccggaact gtctgctgaa gcaaacccgt
    54601 tccgtaatcg tctggcagag tctgaagcca gcaacgatca ggcaccggtg cagatgcctc
    54661 gcgactattc tgaaggcgca tccggcctgc tgcgtactgg cgcgaagcgc gactaattta
    54721 tttttcgggc gcagccattg cgccctcctc ttctctccct ccccgactat catttaatct
    54781 ggtgtctcat tgttagccgt ctgaaaattc aataacatca aactgttttg aatctctttt
    54841 cttatcattc aggtacgaga gcaggaataa tgaaaaaaca accccagctg ttgagtgcat
    54901 tagcgttaag tgtcgggtta actctctcgg cgtcatttca ggccgtcgcg tcgattccag
    54961 gccaggttgc cgatcaggcc cctctcccca gtctggctcc aatgctggaa aaagtgcttc
    55021 cggcagtggt gagcgtacgg gtggaaggaa cggccagtca gggacagaaa atcccggaag
    55081 aattcaaaaa gttttttggt gatgatttac cggagcaacc tgcacaaccc ttcgaaggtt
    55141 taggctccgg tgtcatcatc aacgccagta aaggctatgt gctgaccaac aaccatgtga
    55201 ttaatcaggc acagaaaatc agtattcagc tcaatgatgg gcgcgagttt gatgcaaaac
    55261 tgattggtag cgatgaccag agcgatatcg ccctgttaca aattcaaaac ccgagcaaat
    55321 taacgcaaat cgctattgcc gactccgata aattgcgcgt cggtgatttt gccgtagcgg
    55381 tcggtaaccc atttggcctt gggcaaaccg ccacctctgg cattgtttcc gcattaggac
    55441 gcagcgggtt gaatcttgaa ggtctggaaa actttatcca gacagatgct tccattaacc
    55501 gcggtaactc cggcggtgca ctgttaaacc ttaacggtga gttaattggc atcaacactg
    55561 caatccttgc gcctggcggc gggagcgtcg ggattggatt tgccatcccc agtaatatgg
    55621 cgcgaacact ggcgcagcag cttatcgact ttggtgaaat caaacgcggt ttgttaggca
    55681 tcaaaggcac cgagatgagt gccgatatcg ccaaagcctt caaccttgac gtgcagcgtg
    55741 gcgcgtttgt cagcgaagtg ttgccaggtt ctggctcggc gaaagcgggc gtcaaagcgg
    55801 gcgatattat taccagcctc aacggcaaac cgctgaatag ctttgctgag ttgcgctctc
    55861 gtatcgcgac caccgagccg ggcacaaaag tgaaacttgg cctgctgcgt aacggcaaac
    55921 cactggaagt agaagtgacg ctcgatacca gcacctcttc gtcggccagc gctgaaatga
    55981 tcgcgccagc gctggaaggt gcaacgttga gcgatggtca gctaaaagat ggcggcaaag
    56041 gtattaagat cgatgaagtt gtcaaaggaa gcccagctgc tcaggctggc ttgcaaaaag
    56101 acgatgtgat cattggcgtc aaccgcgatc gggtgaactc gattgctgaa atgcgtaaag
    56161 tgctggcggc aaaatcggcc atcatcgccc tgcaaattgt acgcggcaat gaaagcatct
    56221 atctgctgat gcgttaatgt cgtaaaccgg gcatcaggct tacgtgtgat gtccggttaa
    56281 ctcgtggtat gctgctgccg ttcccttttt taatgacgcc tccatcatgt ttgtgaagct
    56341 cttacgttcc gttgcgattg gattaattgt cggcgctatt ctgctggttg ccatgccttc
    56401 gctgcgcagc cttaacccgc tttccactcc gcaatttgac agtaccgatg agacgcctgc
    56461 cagctataat ctggcggttc gccgcgccgc gccagcggtg gttaacgttt acaaccgtgg
    56521 tttgaacacc aactctcaca accagcttga gatccgcacc ctgggatccg gtgtaatcat
    56581 ggatcaacgc ggttatatca tcaccaataa acacgtcatc aacgacgccg atcagatcat
    56641 cgtcgcctta caggatggtc gtgtatttga agcattgctg gtgggatctg actctctaac
    56701 cgatctggcg gtacttaaaa ttaatgccac tggcggttta cctaccattc caatcaatgc
    56761 acgtcgcgta ccgcatattg gcgacgtggt actggcgatc ggtaacccgt acaacctcgg
    56821 gcagaccatt acccagggga ttattagtgc cacgggtcga attggtctga acccgaccgg
    56881 gcggcaaaac ttcctgcaaa ccgatgcttc cattaaccac ggtaactctg gcggcgcgct
    56941 ggtgaactcg ctgggcgaac tgatgggcat taacacgctg tcgtttgata agagtaacga
    57001 tggcgaaacg ccggaaggta tcggctttgc gattcctttc cagttagcaa ccaaaattat
    57061 ggataagctg atccgcgatg gtcgcgtgat ccgcggctac attggcattg gcggacgtga
    57121 gatcgcaccg ctgcacgcac agggcggtgg tatagatcaa ctgcaaggga tcgtggttaa
    57181 tgaagtgtca cctgacggcc cggcggcgaa tgcgggtatt caggtcaacg atctgattat
    57241 ttcggtggat aacaaaccgg ccatctctgc tctggagacg atggatcagg tggcggaaat
    57301 tcgccctggt tcggtgatcc cggttgtagt gatgcgtgat gataagcagt taacgctgca
    57361 ggtcaccatt caggaatatc cggcaaccaa ttaagtcgag tgctcaaaac aaaaaaccgg
    57421 agtctgtg
//
LOCUS       DAZPLI010000010        53401 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZPLI010000010 DAZPLI010000000
VERSION     DAZPLI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.010"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..849)
                     /gene="melB"
                     /locus_tag="ACHUU3_000554"
     CDS             complement(<1..849)
                     /gene="melB"
                     /locus_tag="ACHUU3_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR2080724.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTL"
     gene            complement(952..2307)
                     /gene="melA"
                     /locus_tag="ACHUU3_000555"
     CDS             complement(952..2307)
                     /gene="melA"
                     /locus_tag="ACHUU3_000555"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR2080725.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2590..3498
                     /gene="melR"
                     /locus_tag="ACHUU3_000556"
     CDS             2590..3498
                     /gene="melR"
                     /locus_tag="ACHUU3_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR2080726.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3697..5964
                     /gene="adiA"
                     /locus_tag="ACHUU3_000557"
     CDS             3697..5964
                     /gene="adiA"
                     /locus_tag="ACHUU3_000557"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR2080727.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            6289..7050
                     /gene="adiY"
                     /locus_tag="ACHUU3_000558"
     CDS             6289..7050
                     /gene="adiY"
                     /locus_tag="ACHUU3_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR2080728.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            7187..8524
                     /gene="adiC"
                     /locus_tag="ACHUU3_000559"
     CDS             7187..8524
                     /gene="adiC"
                     /locus_tag="ACHUU3_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR2080729.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            8628..10271
                     /gene="eptA"
                     /locus_tag="ACHUU3_000560"
     CDS             8628..10271
                     /gene="eptA"
                     /locus_tag="ACHUU3_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR2080730.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            10268..10936
                     /gene="basR"
                     /locus_tag="ACHUU3_000561"
     CDS             10268..10936
                     /gene="basR"
                     /locus_tag="ACHUU3_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR2080731.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            10937..12037
                     /gene="pmrB"
                     /locus_tag="ACHUU3_000562"
     CDS             10937..12037
                     /gene="pmrB"
                     /locus_tag="ACHUU3_000562"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR2080732.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(12013..12102)
                     /gene="pmrR"
                     /locus_tag="ACHUU3_000563"
     CDS             complement(12013..12102)
                     /gene="pmrR"
                     /locus_tag="ACHUU3_000563"
                     /inference="COORDINATES: protein motif:HMM:NF033224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR2080733.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(12214..13716)
                     /gene="proP"
                     /locus_tag="ACHUU3_000564"
     CDS             complement(12214..13716)
                     /gene="proP"
                     /locus_tag="ACHUU3_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR2080734.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(13980..14846)
                     /locus_tag="ACHUU3_000565"
     CDS             complement(13980..14846)
                     /locus_tag="ACHUU3_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR2080735.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(14843..17071)
                     /gene="crfC"
                     /locus_tag="ACHUU3_000566"
     CDS             complement(14843..17071)
                     /gene="crfC"
                     /locus_tag="ACHUU3_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR2080736.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            17473..17808
                     /locus_tag="ACHUU3_000567"
     CDS             17473..17808
                     /locus_tag="ACHUU3_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR2080737.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            17966..18409
                     /gene="yjdN"
                     /locus_tag="ACHUU3_000568"
                     /pseudo
     CDS             17966..18409
                     /gene="yjdN"
                     /locus_tag="ACHUU3_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            18542..19330
                     /gene="phnC"
                     /locus_tag="ACHUU3_000569"
     CDS             18542..19330
                     /gene="phnC"
                     /locus_tag="ACHUU3_000569"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR2080738.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESAGIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            19355..20371
                     /gene="phnD"
                     /locus_tag="ACHUU3_000570"
     CDS             19355..20371
                     /gene="phnD"
                     /locus_tag="ACHUU3_000570"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR2080739.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            20497..21276
                     /gene="phnE"
                     /locus_tag="ACHUU3_000571"
     CDS             20497..21276
                     /gene="phnE"
                     /locus_tag="ACHUU3_000571"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFR2080740.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLCKHFI"
     gene            21297..22022
                     /gene="phnF"
                     /locus_tag="ACHUU3_000572"
     CDS             21297..22022
                     /gene="phnF"
                     /locus_tag="ACHUU3_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFR2080741.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            22023..22475
                     /gene="phnG"
                     /locus_tag="ACHUU3_000573"
     CDS             22023..22475
                     /gene="phnG"
                     /locus_tag="ACHUU3_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFR2080742.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            22472..23056
                     /gene="phnH"
                     /locus_tag="ACHUU3_000574"
     CDS             22472..23056
                     /gene="phnH"
                     /locus_tag="ACHUU3_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFR2080743.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            23056..24120
                     /gene="phnI"
                     /locus_tag="ACHUU3_000575"
     CDS             23056..24120
                     /gene="phnI"
                     /locus_tag="ACHUU3_000575"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFR2080744.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            24113..24958
                     /gene="phnJ"
                     /locus_tag="ACHUU3_000576"
     CDS             24113..24958
                     /gene="phnJ"
                     /locus_tag="ACHUU3_000576"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFR2080745.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            24955..25713
                     /gene="phnK"
                     /locus_tag="ACHUU3_000577"
     CDS             24955..25713
                     /gene="phnK"
                     /locus_tag="ACHUU3_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFR2080746.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            25824..26504
                     /gene="phnL"
                     /locus_tag="ACHUU3_000578"
     CDS             25824..26504
                     /gene="phnL"
                     /locus_tag="ACHUU3_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFR2080747.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            26501..27637
                     /gene="phnM"
                     /locus_tag="ACHUU3_000579"
     CDS             26501..27637
                     /gene="phnM"
                     /locus_tag="ACHUU3_000579"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFR2080748.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            27637..28194
                     /gene="phnN"
                     /locus_tag="ACHUU3_000580"
     CDS             27637..28194
                     /gene="phnN"
                     /locus_tag="ACHUU3_000580"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFR2080749.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            28181..28615
                     /gene="phnO"
                     /locus_tag="ACHUU3_000581"
     CDS             28181..28615
                     /gene="phnO"
                     /locus_tag="ACHUU3_000581"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFR2080750.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            28617..29375
                     /gene="phnP"
                     /locus_tag="ACHUU3_000582"
     CDS             28617..29375
                     /gene="phnP"
                     /locus_tag="ACHUU3_000582"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFR2080751.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(29522..29851)
                     /gene="yjdP"
                     /locus_tag="ACHUU3_000583"
     CDS             complement(29522..29851)
                     /gene="yjdP"
                     /locus_tag="ACHUU3_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFR2080752.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(29920..30237)
                     /locus_tag="ACHUU3_000584"
     CDS             complement(29920..30237)
                     /locus_tag="ACHUU3_000584"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFR2080753.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            30490..32475
                     /gene="yjcS"
                     /locus_tag="ACHUU3_000585"
     CDS             30490..32475
                     /gene="yjcS"
                     /locus_tag="ACHUU3_000585"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFR2080754.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            32684..32959
                     /locus_tag="ACHUU3_000586"
     CDS             32684..32959
                     /locus_tag="ACHUU3_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFR2080755.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            32978..34009
                     /gene="mdtN"
                     /locus_tag="ACHUU3_000587"
     CDS             32978..34009
                     /gene="mdtN"
                     /locus_tag="ACHUU3_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR2080756.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            34009..36060
                     /gene="mdtO"
                     /locus_tag="ACHUU3_000588"
     CDS             34009..36060
                     /gene="mdtO"
                     /locus_tag="ACHUU3_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR2080757.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            36057..37523
                     /gene="mdtP"
                     /locus_tag="ACHUU3_000589"
                     /pseudo
     CDS             36057..37523
                     /gene="mdtP"
                     /locus_tag="ACHUU3_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
     gene            37721..39868
                     /gene="fdhF"
                     /locus_tag="ACHUU3_000590"
     CDS             37721..39868
                     /gene="fdhF"
                     /locus_tag="ACHUU3_000590"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:38138..38140,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFR2080758.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMDHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            39962..40651
                     /gene="yjcO"
                     /locus_tag="ACHUU3_000591"
     CDS             39962..40651
                     /gene="yjcO"
                     /locus_tag="ACHUU3_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR2080759.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(40730..42043)
                     /gene="gltP"
                     /locus_tag="ACHUU3_000592"
     CDS             complement(40730..42043)
                     /gene="gltP"
                     /locus_tag="ACHUU3_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR2080760.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHRELLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(42385..42981)
                     /gene="nrfG"
                     /locus_tag="ACHUU3_000593"
     CDS             complement(42385..42981)
                     /gene="nrfG"
                     /locus_tag="ACHUU3_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR2080761.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(42978..43361)
                     /gene="nrfF"
                     /locus_tag="ACHUU3_000594"
     CDS             complement(42978..43361)
                     /gene="nrfF"
                     /locus_tag="ACHUU3_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR2080762.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(43354..45072)
                     /locus_tag="ACHUU3_000595"
     CDS             complement(43354..45072)
                     /locus_tag="ACHUU3_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR2080763.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(45092..46048)
                     /gene="nrfD"
                     /locus_tag="ACHUU3_000596"
     CDS             complement(45092..46048)
                     /gene="nrfD"
                     /locus_tag="ACHUU3_000596"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR2080764.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(46045..46716)
                     /gene="nrfC"
                     /locus_tag="ACHUU3_000597"
     CDS             complement(46045..46716)
                     /gene="nrfC"
                     /locus_tag="ACHUU3_000597"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR2080765.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(46713..47279)
                     /gene="nrfB"
                     /locus_tag="ACHUU3_000598"
     CDS             complement(46713..47279)
                     /gene="nrfB"
                     /locus_tag="ACHUU3_000598"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR2080766.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(47324..48760)
                     /gene="nrfA"
                     /locus_tag="ACHUU3_000599"
     CDS             complement(47324..48760)
                     /gene="nrfA"
                     /locus_tag="ACHUU3_000599"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR2080767.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            49154..51112
                     /gene="acs"
                     /locus_tag="ACHUU3_000600"
     CDS             49154..51112
                     /gene="acs"
                     /locus_tag="ACHUU3_000600"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFR2080768.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLTCGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            51312..51625
                     /locus_tag="ACHUU3_000601"
                     /pseudo
     CDS             51312..51625
                     /locus_tag="ACHUU3_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            51622..53271
                     /gene="actP"
                     /locus_tag="ACHUU3_000602"
     CDS             51622..53271
                     /gene="actP"
                     /locus_tag="ACHUU3_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR2080769.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
BASE COUNT        12838 a        14702 c        13890 g        11971 t
ORIGIN      
        1 taacgtcacc aggttagcag ctcccgcata cgacagataa taggggaaca aatccgcatc
       61 accgataaca tatgagaaat aatagatagc aaatccggta ataatgttgc tggctacatt
      121 ataagcaaga gccataccca agaggcatga aagctgatcg tttttataaa ttagcgcaac
      181 gatggcttta agtgtcagat ggcttccttc agcagacggt tgattgtctg acgaaaagac
      241 ttcatgcaca ttgcgcagag tgatgatggt tgaaacaata aaaaaggcga tcagtaccag
      301 tgtgaacatc tgaaagccaa atccccgatc gccaccgccg acataattaa caaatggtag
      361 cgtcacacct gccgtaacaa agcccgccag actggcaaaa aaacgcggat aaggaaccag
      421 ttgttcacgc tcacgtttat cgagcgtgat ggttggaacc agcgaccaga agggaatatc
      481 cataatggtg taagtcatgc cccagaggat gtaggtcacg caaacaaaga caatctgagt
      541 agtaccttca aacagatgcg cactaaagag gagaaataag attacagagt ttgccaacgt
      601 accgatcagg atccagggtt tgaacttacc ccatcgcgat cgcgtagcat ttacaatcca
      661 tcccataatc ggatcgttaa tagcatccca gatcctcgcc accagaaaca aagtaccgac
      721 caaacccaca gacagcccaa cgacatcggt gtagtaatac atgaggtaca tatacacaat
      781 gccgatcgca aaatccttcc cgaacgctcc aaatccataa ctgagttttg tagtcattga
      841 aatgctcata gggtatcggg tcgctgttac caacgcccgc cttctgttat tggtatcgtc
      901 agaggtgtgc gccggaataa atcccccggc gcagtagcgt ttagtcgcgt tttaacggtg
      961 caaccagcct ggcagccagt cgccgtgggc ggcaatcagg tcgtcaacaa gagcatatat
     1021 ttcgtcaatg cccagcacgg cggcagtatg cgggtccatc atcgcggcgt ggtaaacacg
     1081 gtcgcgattt tccgtaagaa tagcttcggt cagcagcgtc tgtacattga tgttggtttg
     1141 catcagggcg gccagatgcg aaggtagcgt accgacttta gtcggctgaa tgccattagc
     1201 atcaaccaga caggctactt ccacgcaaca tccttgtggc aggttatcaa tcaaaccatc
     1261 gttacggacg ttgccgtaaa tcacactcgg ctcgccagtc cagatagcgt tcatgattgt
     1321 gctggcatat tctcgtgacg gtttaatatc aatccgggag gcgtttttat actcctccag
     1381 ctctttatgc cagttcgcca gctgctcgac gcagcgtttc gggtactcat ccagcggtac
     1441 tttataacgc tcaatcaaat cctcacgacc tggcttaata aaccacggcg tgtattcagc
     1501 aaaatgttct gacgattccg taacgaagta gcccagcttt ttgaacattt cgtagcgcac
     1561 aatattctgg cagcgagtat taccatgaat atttggcttc ggtgcctgcc ctgcttcata
     1621 agccgccagc agttccgggt agagattcac gtaactgccg tcggcggttt tacgctccag
     1681 ttccaggtaa aacgccatat ggttgatacc agcgcaacgg taacgcagcg tggacggatc
     1741 gatattgagg tcacgcgcca gctcttccgc cgttccctgc accgaatggc acagccctac
     1801 ctgtttgata tgcggatagc gggcatacat cgcccaggta ttcatcgcca ttgggttaac
     1861 gtagttgagc atggtggcat cggggcagac ttccgtcatg tcctcgcaaa tttgccacag
     1921 atgcggaatg gtacgtagcg cgcgcataat accgcccggc cccaacgtat cggcaatggt
     1981 ttgttccaga ccatgccgct tacagacctc gaaatcagtc accgtgcaag gttcataacc
     2041 gccaatctga aatgccacca cgacaaaatc ggcatcctgt aaggcttctt tctgttgggt
     2101 gtggcaggtg attttgccgc tggcccctgc tgaatccatc agcttacgca ccacaatatg
     2161 cgactcttcc aggcgggtgg gatcaatgtc catcagggca atatgcgccg ttttcagcgc
     2221 ctcgcgatgg aacacatcac caagaatatt tttaacgaaa atcgtcgaac cagcgccgat
     2281 aaatgtaatt ttgggtgcag tcatcatgca gatctcctgg cttgcttgaa taacttcatc
     2341 atggcaggcg aatctgcaga aaacctccgg cttccaggtt gagcattccc ataaactcag
     2401 atcctgcgtg aagcagcagt aaatctgagt ttatggtaat aagttacctg aaaagcagag
     2461 ttttcggcct catgatgctc ggacgctccc ctcgcctctc tgggaagagg gtgaaaaccg
     2521 tgctcccact cgcagtcatc ctccctcact cctgccataa ttctgatatt ccaggaaaga
     2581 gagccatcca tgaatacaga tacgtttatg tgcagcagcg acgaaaaaca gacccgcagc
     2641 ccgctgtcgc tgtattcgga ataccagcgg atggagattg agtttcgcgc accgcatatc
     2701 atgcccacca gccactggca tggtcaagtc gaagtgaatg tgcctttcga tggcgatgtg
     2761 gaatacctga tcaacaatga aaaagtgaat atcaatcagg gtaatatcac gctgttctgg
     2821 gcctgtacac cgcaccaact aacagatacc ggaacctgtc agagcatggc gatttttaat
     2881 ctgccgatgc atctgtttct ctcctggccg ctggataaag acctgattaa ccacgtcact
     2941 cacggcatgg tgatcaaatc actggccaca cagcaactta gcccgtttga agtgcgccgc
     3001 tggcagcagg aattaaacag tccgaacgag caaattcgcc agctcgccat tgatgaaatt
     3061 ggcctgatgc tcaagcgatt tagcctctct ggctgggaac cgattctggt caataaaacc
     3121 tcgcgcacac acaaaaacag cgtctcgcgc catgcgcaat tttatgttag ccagatgctg
     3181 ggctttattg ccgaaaacta tgatcaggcg ctgaccatca acgatgtggc tgagcacgtc
     3241 aaacttaacg ccaactatgc aatggggata ttccagcggg tcatgcaatt gacgatgaaa
     3301 cagtacatta ccgcgatgcg catcaaccac gttcgcgcgt tactgagcga taccgataaa
     3361 agtattctcg atattgccct gacggcaggc tttcgttcaa gtagtcgttt ttacagcacg
     3421 ttcggcaaat atgtcggcat gtcgccgcaa caataccgca aacttagcca acagcgccgc
     3481 cagacgtttc ccggctaaag acgatatcag tatcagccaa aaaaatagtt tagccgtcga
     3541 taataagcag tgaaaataat ttaaaataat cacataacgc attgtttata aaaataaata
     3601 tttcaaaaat gtttgttttt cacgcgcttt acagcccgaa aaggccggaa gatacttgcc
     3661 cgcaacgaag attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc
     3721 gagtttctcc atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta
     3781 agccagcaaa atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc
     3841 tcttcaaacg aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa
     3901 catcaaaacg tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc
     3961 ttcctgttgg gcgatcggga aaaagccctc gccgcaatgg atcgcgacct gctggagctt
     4021 gtcgatgaat tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgctgtt
     4081 gccgcgatga cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa
     4141 tatagcgaca tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt
     4201 accaaaacac ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc
     4261 gatatgggca tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc
     4321 gaaagcgaaa aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc
     4381 ggtacttccg gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg
     4441 gtcgttgacc gtaactgcca taaatccatc gaacaaggct tgatgctgac aggcgcgaaa
     4501 ccggtctaca tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag
     4561 gaaatgcaac ctgaaacctt gcagaagaaa atcagtgaaa gcccgctgac taaagacaaa
     4621 gccgggcaaa aaccgtctta ctgcgtggtg accaactgca cctatgacgg cgtgtgttat
     4681 aacgctaaag aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa
     4741 gcctggtacg gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc
     4801 gaacctggcg atcacaacgg tcctaccgtt ttcgccaccc actccaccca caaactgctg
     4861 aatgcgcttt cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc
     4921 tcccgcttca accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc
     4981 gcatccaacg acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacgcag
     5041 gaagtgatcg acgaagcggt tgatttccgt caggcgatgg cgcggctgta taaagagttc
     5101 accgctgacg gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa
     5161 accggcaaaa cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac
     5221 tgctgggtaa tgcatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg
     5281 agtatgctcg acccgattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa
     5341 ctggaagaaa ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt
     5401 gtgcctaccc gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt
     5461 gggaaatggg gaactctggt taacaccctt tgctccttca aacgccacta tgacgccaac
     5521 acaccgctgg cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac
     5581 atggggattc acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc
     5641 gcacggttga acgaagccta ttccggcctg tcggtggcgg aaatcacccc gcgtgaagcg
     5701 tacaacgcga ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc
     5761 atcgcggcaa actcagttat cccatatccg ccaggaatcc cgatgctgct gtctggtgaa
     5821 aacttcggcg ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac
     5881 caccatttcc ctggatttga acacgaaact gaagggactg aaattattga cggtatttac
     5941 cacgttatgt gtgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac
     6001 attacgccgc ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca
     6061 aacaaaaatg tcatactttt tgcgcggccc caccccgcgc ttttttttgc ctgttattta
     6121 tcctgtaaaa aaatatgtac atgagaaatt actataaaaa tttgtaatat tagtaaaact
     6181 cgttattttt atgcatgttt atattcatca tacaattata taaccatttc ccggtatcgc
     6241 tttgctttag cgagaactgg tcttttattc gcactcagga gtacatgtat gaggatttgc
     6301 agcgaccaac cttgtattgt tttattgact gaaaaagatg tctggataag ggtgaatggg
     6361 aaagaaccta ttagccttaa agctaaccat atggcgttat taaattgtga aaataatatt
     6421 atcgacgtct cctctcttaa caacactttg gttgctcata ttagtcacga catcatcaaa
     6481 gattacctcc ggtttctgaa taaagatctc tcgcaaatac cagtatggca acgtagcgct
     6541 acgcccatac tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc cgcgcaacac
     6601 agcatgatgc ccgcagaaac tgagtcagaa aaggaacgaa cacgcgcatt attattcacg
     6661 gtgctatccc gttttctcga cagtaaaaaa ttcctttcat taatgatgta tatgttacgt
     6721 aattgtgtaa gtgacagcgt ttatcaaatt attgaaagcg atattcacaa agactggaat
     6781 cttagtatgg tagccagttg tttatgtctt agcccaagtc tgttaaagaa aaagctgaaa
     6841 agcgaaaaca ccagttatag ccaaataatc accacctgcc gcatgcgtta tgccgtaaat
     6901 gaattaatga tggacggtaa aaatatctct caggtatcac agtcctgcgg ctacaacagt
     6961 acgtcgtact ttatttctgt ctttaaagac ttctacggta tgacgccgct gcattatgtt
     7021 agtcagcaca gagaacgaac tgtcgcctga tttttaacct taacgaagag ctatattaat
     7081 aacggcatca gcgataaccc ggtcgataat aattcaacta tcgaatgcag gcgtatgata
     7141 tgacgtaatt tattgtcacg aagctcgcct tcgcaggagt ttaattatgt cttcggatgc
     7201 tgatgctcac aaagtgggct taatccccgt caccctgatg gtgtcgggga atattatggg
     7261 gtcaggtgtt tttctgttac ctgcaaacct ggcctccact ggcgggattg ccatttatgg
     7321 atggttggtg acgattatcg gtgcgctggg gctctcgatg gtatacgcca aaatgtcgtt
     7381 cctcgatcca agtcctggtg gttcttacgc ttacgcccgc cgctgctttg gcccgtttct
     7441 cggttatcaa accaacgtcc tctactggct ggcctgctgg atcggcaata tcgctatggt
     7501 ggtcattggc gtaggatatt taagttactt cttcccgatt ctgaaagatc cattggtatt
     7561 aaccatcacc tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg tcggtccgaa
     7621 aatgatcacc cgtgtgcagg ccgttgccac cgtgctggcg ctgatcccca tcgtcgggat
     7681 tgccgtattt ggctggttct ggttccgtgg tgaaacctat atggcggcat ggaacgtcag
     7741 cggcctgggc accttcggtg cgattcaaag tacccttaac gttacgctgt ggtcgttcat
     7801 cggtgtggaa agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac gcaatgtccc
     7861 tatcgccacc attggtgggg tattgattgc cgccgtttgc tatgtgctct ccaccaccgc
     7921 gattatgggg atgatcccta atgccgcact gcgcgtttct gcctcgccat tcggtgatgc
     7981 cgcacggatg gcgttgggtg acaccgctgg ggccattgtt tccttctgcg cagctgcggg
     8041 ttgcttaggt tcactgggcg gctggacgtt gctggcgggt caaacggcga aagccgctgc
     8101 cgatgacgga ctgttcccac cgatttttgc ccgtgtaaat aaagcgggta cgccagtggc
     8161 ggggctgatt atcgtcggta ttttgatgac catcttccag ctcagtagca tttcgccaaa
     8221 cgcaaccaaa gagttcggtc tggtatcttc cgtgtcggtc atctttacac tggtgccata
     8281 tctttacacc tgtgcggcgt tactgctgct cggccacggt cactttggta aagcacgccc
     8341 ggcatatctg gctgttacta ccattgcctt cctctactgc atctgggccg tggtggggtc
     8401 cggggcgaaa gaggttatgt ggtcatttgt caccctgatg gtcatcaccg ccatgtatgc
     8461 gctgaattac aaccggctac ataaaaaccc gtatccctta gatgcaccaa taagcaaaga
     8521 ttaattcccc ttaatccagc aaacataaaa gccaacctta agaacttaag gttggcttaa
     8581 ttttgctttg cgagcatatg cgcactttgt tcgatggaaa caccgtgatg ttgaagcgcc
     8641 tactaaaaag cccctctttg aatttactcg cctggctatt gttggccgct ttttatatct
     8701 ctatctgcct gaatattgcc ttttttaaac aggtgttgca ggcgctgccg ctggattcgc
     8761 tgcataacgt actggttttc ttgtcgatgc cggtcgtcgc tttcagcgtg attaatattg
     8821 tcctgacact aagctctttc ttatggctta atcgaccact ggcctgcctg tttattctgg
     8881 ttggcgcggc tgcacaatat ttcataatga cttacggcat cgtcatcgac cgctcgatga
     8941 ttgccaatat tattgatacc actccagcag aaagttatgc gctgatgaca ccgcaaatgt
     9001 tattaacgct gggattcagc ggcgtgcttg ctgcgctgat tgcctgctgg ctaaaaatca
     9061 aacctgccac ctcgcgtctg cgcagtgttc ttttccgtgg agccaatatt ctggtttctg
     9121 tactgctgat tttgctggtc gccgcactgt tttataaaga ctacgcctcg ttgttccgca
     9181 ataacaaaga gctggtgaaa tccttaagcc cctctaacag cattgttgcc agctggtcat
     9241 ggtactccca tcagcgactg gcaaatctac cgctggtgcg aattggtgaa gacgcccacc
     9301 gcaacccgtt aatgcagaac gaaaaacgta aaaatttgac catcctgatt gtcggcgaaa
     9361 cctcgcgggc ggagaacttc tccctcaacg gctacccgcg tgaaactaac ccgcggctgg
     9421 cgaaagataa cgtggtctat ttccctaata ccgcatcttg cggcacggca acggcagttt
     9481 cagtaccgtg catgttctcg gatatgccgc gtgagcatta caaagaagag ctggcacagc
     9541 accaggaagg cgtgctggat atcattcagc aagcgggcat caacgtgctg tggaatgaca
     9601 acgatggcgg ctgtaaaggt gcctgcgacc gcgtgcctca ccagaacgtc accgcgctga
     9661 atctgcctgg tcagtgcatc aacggcgaat gctatgacga agtgctgttc cacgggctgg
     9721 aagagtacat caataacctg cagggtgatg gcgtgattgt cttacacacc atcggcagcc
     9781 acggtccgac ctattacaac cgctatccgc cgcagttcag gaaatttacc ccaacctgcg
     9841 acaccaatga gatccagacc tgtaccaaag agcaactggt gaacacttac gacaacacgc
     9901 tggtttacgt cgactatatt gttgataaag cgattaatct gctgaaagaa catcaggata
     9961 aatttaccac cagcctggtt tatctttctg accacggtga atcgttaggt gaaaatggca
    10021 tctatctgca cggtctgcct tatgccatcg ccccggatag ccaaaaacag gtgccgatgc
    10081 tgctgtggct gtcggaggat tatcaaaaac ggtatcaggt tgaccagaac tgcctgcaaa
    10141 aacaggcgca aacgcaacac tattcacaag acaatttatt ctccacgcta ttgggattaa
    10201 ctggcgttga gacgaagtat taccaggctg cggatgatgt tctgcaaact tgcaggagag
    10261 tgagtgaatg aaaattctga ttgttgaaga cgatacgctg ttattgcagg gactgattct
    10321 ggcggcgcaa accgaaggct acacgtgcga tggcgtaaca accgcgcgga tggcggaaca
    10381 aagccttgag gccggtcatt acagcctggt ggtactggat ttagggttac ccgacgaaga
    10441 tggactgcat tttctcgccc gtatccggca gaaaaaatac accctgccgg tactgatcct
    10501 caccgctcgc gatacgctga ccgacaaaat cgccgggctg gatgtcggtg ccgatgacta
    10561 tctggtgaag ccttttgcgc tggaagagtt acacgcccgt atccgcgccc tgctacgacg
    10621 ccataataat cagggcgaaa gtgagctgat tgttggcaat ctgacgctga atatgggtcg
    10681 ccgtcaggta tggatgggcg gtgaagagtt gattctgacg cccaaagaat atgctctgct
    10741 gtcacggtta atgctcaaag caggcagtcc ggtacatcgg gaaattctct acaacgacat
    10801 ctataactgg gacaatgaac cctcgaccaa caccctggaa gtgcatatcc acaatctgcg
    10861 cgacaaagtg ggcaaagccc gtatccgcac cgtgcgcggc tttggctata tgctggtcgc
    10921 gaatgaggaa aactaattga atctgatgcg ttttctgcgc cgaccaatat cgctgcgcca
    10981 acggctgata ttgaccatcg gggccatttt gttggtgttt gagctgatca gcgtcttctg
    11041 gctatggcat gaaagtaccg agcagattca gctgtttgag caggcgctac gcgacaatcg
    11101 caacaacgat cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc tgattgtccc
    11161 cggcgtcttt atggtcagcc tgacgctatt tatctgctat caggcggtac gccgcatcac
    11221 ccgcccgctg gcggagctgc aaaaagagct ggaagcgcgc accgccgaca acttaacgcc
    11281 cattgccatt cacagcgcca ccctcgaaat cgaagcggtg gtttcggcgt tgaacgatct
    11341 ggtcagtcgc ctgacgagca cgctggataa cgaaaggtta tttaccgctg acgtcgcgca
    11401 cgaactgcga acgccactgg cgggggtgcg tttgcatctg gaactgctgg cgaaaacgca
    11461 tcacattgat gtagcaccgt tagtggcacg gcttgatcag atgatggaga gcgtctccca
    11521 gctgctgcaa ctggcgcgtg ccggacagtc attttcttcc ggtaattatc aacatgtaaa
    11581 actgctggaa gatgtcattc tcccctcgta tgacgaactc agtaccatgc tcgaccagcg
    11641 acagcaaacc ctgctattgc cagagagcgc cgccgacatc accgttcagg gtgatgcaac
    11701 cctgctgcgg atgttattgc gaaacctggt agaaaacgcc catcgttaca gcccacaagg
    11761 cagcaacatt atgattaagc tgcaagaaga tggcggagcg gtcatggccg ttgaagatga
    11821 aggaccgggt attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg tacgtatgga
    11881 cagccgttat ggcgggattg gtctggggtt aagtattgtc agccgcatta cacagttgca
    11941 tcacgggcag tttttcctgc aaaaccggca agagacttcc ggcacgcggg cctgggtacg
    12001 gctgaagaaa gatcagtacg tagcaaacca gatataaaga aagctgctga ccaccagcac
    12061 actgaacacg gtagttaaac tttcataaac acggtttttc attgcactct cctccgttaa
    12121 cctggaggag agtatgcgcg tcagagatta agtgaacctt aagagttcaa ttggcctgat
    12181 aagacagcgt cacatcaggc catccgtttc agcttattca tcaattcgcg gatgttgctg
    12241 caccaggcgg gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat catcgatttt
    12301 ctgctcgata ttatcgtaat gctcgacgag aatttccttc gcttcctgta tatctgacgc
    12361 cgccggtgtc gcacctttca acggacgatt tgccgtctct ttcatggtta cgccggtgat
    12421 taaaccaatc accgccacta ccatcaggta ataggcaggc atcatcagat tctgcgagct
    12481 ttcgaccagc caggccgcca gtgttggcgt cagaccggca accagcaccg aaatattaaa
    12541 tgccgccgcc agcgcgctgt aacggatatg cgtcgggaac atcgctggca aggtagaagc
    12601 cataacgccc gtaaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag
    12661 gccgatgacg ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagggcaac
    12721 actaccaagt agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg
    12781 ctggacaaac agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga
    12841 gtaatgcagg ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac
    12901 gttggtggca attaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat
    12961 ctctttaaac gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt
    13021 atcaacatgc tgctggaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat
    13081 aatccctaac ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc
    13141 ttcgccgaca atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat
    13201 agaaccgaag tccagccagc tgcccataaa gccacgttta cggtcagggg agtattccgc
    13261 aacaaatatc gacgccccgg tatattcacc gccgaccgag aaaccttgtg ccatcttaca
    13321 gatcaacagc agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat
    13381 acagaacgta ctgatcgaca taatcacaat agtgatagcg aggatcttct ggcgaccata
    13441 tttatcgccc aacataccaa agaagagccc gccaagcggt cgaatcagaa agggaacgga
    13501 gaaagtggca agtgcagcaa ccatctgcac gctggggtca gcccccggga aaaaaacttt
    13561 acctaatgcg taagcaacaa aaccataaac accaaaatcg aaccattcca ttgcattacc
    13621 cagtgatgct gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg
    13681 aagggtaatc ggttttactt ttttcctttt cagcatagct ttcctcgcag agttggccca
    13741 tttccgcaaa cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg
    13801 atgcaatctc tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca
    13861 atcaaaccag ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagtgttta
    13921 aatttgtgat caacttcaca catggatttc gtttaggact cattgatgta actgattatt
    13981 tacgccaaat ttacagagaa gatttcctcg accattgccc ccaccaaacg ctgcgctgaa
    14041 cagagtcgtg gcatccccag cgcgactgtt tgccagcgtt gggtcactga ccagcaaacg
    14101 ggctgtttat ctgccgtgga ccactttccc agccaactta aaaaatcagc atgctcccct
    14161 cccgaagagg caaaccactg atggtaatag tgacgcgtgg tcggtgccgc attgatactc
    14221 tctgccagat gttgtgtcgc tttctcgcgc aaatcaccca gtaacaacac caccgcttgc
    14281 gggcttccca gacggcaggc atcgccaaat gcaccccagc gcaactcctc cagaacgata
    14341 aaacagcgcc caggcaacga ccaggcgtta tacgccccag cacgccagcg ggtaaaaatt
    14401 tgctcgctct gatcacctgc ctgcaccagt aggcctcgtt gcgttaacgc cttttccttc
    14461 cagcgacaac gggctgaaaa ccactcatac aaggcggcaa tttgctgggg gagctccggg
    14521 aaagcagagt gctggcggtg atccattttt tgcagcagcg ccagatgctg cgcgatctgc
    14581 gtcagcgcca gatgaccggg atccagcatc gacgccagtt tttccaccat tgtcagtcgc
    14641 aggctaagcg gcgcatttaa catgccgccg ttcgtccatg tctgaagctg cacacgcgaa
    14701 agaagttcct gcatcagtcg ttcacgaaac tgctgctgat gggcttgtgg aaggcgggca
    14761 tcatcgccct gcgccagatc cactaaaaag cggggataaa ccgactccag cacgcgaccg
    14821 gggccgtcca gtaacgtctt ggtcaatatc gttctgccgt gaaaagtgtt tgaatatcat
    14881 cgcgtaacag ctgggcgtcg gtgtaaatcc agccgtgagt catcacagtc tgctgcaatt
    14941 gctgctgcat cagcctgacc accgattcat tttgttgacg cagagccagg ctttcgcgta
    15001 aacgcgtctg taattccgtc aaacatgaag cgaactcagc gcaaaaagta ttcatgcctg
    15061 ccgtaacaga ttcatcgacc tgctctgcca gaactttagc catttgttgg caataaagat
    15121 cgacttctgc gcttaaagct cgttgcaaca cactgtaatc aaccgtctct gtcggggatt
    15181 tctcatttcc ccgtccccag tcgggctgat tcaaccagcg cgaaaaagtc tcacgcacaa
    15241 cgcctaaacg cgtgctctgc tcgtccgttg catcctggcg cgaaatgact tcactgaaca
    15301 gctggcgagt gttgacgtgg ggaactactc cgtgaaaaac aggaaaatga aacccaggac
    15361 gaaaccctga ctcgctcaat tccattttga cttgttgctc aatgggccga ataacatcgg
    15421 ttaacacccg gcaaagggtg gattcaagct cggcaaaacg cagcgtaaag tcgcgactga
    15481 tggtgttctg cgccgtctgt aacagtgtct cacagcaggt acgaatctcg cttaacggct
    15541 ccgaatcatc ctgaaacaag gcggctaact gcgcattcag cgcatcttgc tgttgacgca
    15601 gaaagtggtt ggcggaggtc agggccagct cgatttcatg tttaatctcg ccgctcacct
    15661 gcgcctgatt gagttgcaat agctgcaaac tttcttcgac ctgatggata ttttgccgca
    15721 attgttcaca agcgacgttt aacccgtgcg cacgaaaatc caggtattcc cgcgcctgct
    15781 gcgcgtaatt caacagttta tgcgcagcag atcgcagagc atacaacgag gcgttagcgt
    15841 aagcggcatg aagcaacgcc tgaattggct gggcgaacag cgaatcttcc cacaactgat
    15901 cggcagcatg acgaatatgt tcgaggtccg ccagatcggc atgacgccag cgcctgccga
    15961 gcgcggcatg ggcaaaatct tccacccagc gttgttgctc tggcgctggt aacttaccgt
    16021 tgttggctaa ctcatggcgc gctcgatttg ccaagtagcc ccacatcgac gacaccggaa
    16081 atatctgctg tggcgtaata cagcctttca tcagcgtccc ggaaatcagt gcccgcacct
    16141 ggtcggcgtc gtcactgtta cgatcctgtt gatcgaactt attgaccagc acatacagtg
    16201 gcaccgattg ccccaccgcc aaaatcgcct cacggacctc ttcatcggag atcgatttca
    16261 gttgcgtata atccagcacc gccagtaccg ccgaggcgcg tgccagctgc tggttaagca
    16321 ttttttgcag atgcggttgc ccggcttcat ttggcccggg ggtatccagt aacgtcaact
    16381 gaccgggata actctccagc cccgccagat ggacaaactc cacttcaatc acgggaatat
    16441 gctcaatggc ggcgtaagca gaaaaaggaa aatcgacgtc cagcgccttc gccagtcgca
    16501 ctaaatcatt caaacttttc agacaatgaa aaataggctg ggcacccaga taatattttt
    16561 cgaaagcgac gccattttcg atccgctgca taagcgcacg catatcttta tctatttcca
    16621 gcacatcggt cagatgctta atatcgcaat cacgcaggcg ctgttgtaat tgttgaatta
    16681 aacaatcgat tggcgcgaca tgtgaaaaat gcagtaccgg ttccttttgc ccgggcgtat
    16741 ggcgaataag cgtcggcagc gcagtcattg ggcgattacg attaggcaga acctccgtac
    16801 caacaatggc attaatggtg gttgatttcc ctgctttcat agtaccgaca atcgccagca
    16861 ccatttccag tcgggaaatt ttactcaact cattattcag catcgcgtga cgttcggcga
    16921 tattaggctg actccagggt aaagccagtt gtggcgcgtc gtctccgggt acagagagag
    16981 gcattttttc cagtaactgc aactgttggc gagaaagctg taacaggcgt tcagcctcct
    17041 gacttaactc atacagggtc tgtgtgtaca tagaaaattc ttccttaaag caaattttgt
    17101 tacttttatt tagccagatt gtttttgagt tctgtttttg gcttttataa ttactgcaag
    17161 aaataatttt atatttagtg tgttgttttt tatcagaata aataacgtct tctgatgcgt
    17221 ttaaaacgtc agaaagataa aaatatcatg tgaataaaaa aaagaacaag tagagcatta
    17281 acattatctt aaataataaa tagaggcaaa aagattattt tctttttgcg tttcctttca
    17341 aatgaaaacg atcgtcgtct aaaatcagca gtacccccga caaactcagg gattttgtgt
    17401 ataattgcgg cctttttcgg caatctgccg ttttttggcg cttttgccct gctgactttt
    17461 gaggaaatcc acatgtcatt accacactgc ccaaaatgca actccgaata cacttacgaa
    17521 gataacggca tgtacatctg cccggaatgt gcctacgaat ggaacgacgc agaacctgca
    17581 caggaaagcg acgagctgat cgttaaagat gctaacggca atctgctggc tgacggcgac
    17641 agcgttacca tcattaaaga tctgaaggtg aaaggtagct cttcgatgct gaaaattggc
    17701 accaaagtga aaaacatccg cctggttgaa ggcgaccata acatcgattg caaaatcgac
    17761 ggttttggtc cgatgaaact gaaatctgag tttgtgaaaa agaactgatt gtattgtgat
    17821 cggtaagccg gataaggcgc tcgcgccgca tccggcaacg atgccgactg cctgatgcga
    17881 cgcttgcgcg tcttatcagg cagcctctca tctgtataaa tttcgaacta cacttaattg
    17941 gcttctctta actgaggtca ccatcatgcc gttaagtccc tacctctctt ttgccggtaa
    18001 ctgtgccgac gcaattgctt attatcaacg tacgttgggc gctgaactgc tctataaaat
    18061 cagcttcggc gaaatgccaa aatcagcgca ggacagcgcc gagaactgcc cttccggaat
    18121 gcaatttccc gataccgcca tcgcccacgc caatgtgcgc attgccggaa gcgacatcat
    18181 gatgagcgat gccatgccgt caggaaaagc cagctactcc ggctttacgc tggtgctcga
    18241 ttcgcaacag gtcgaagaag gaaaacgctg gtttgacaat cttgccgcta acggaaaaat
    18301 cgaaatggcc tggcaggaaa ctttctaggc gcatggcttt ggcaaagtca ccgataaatt
    18361 tggcgtaccg tggatgatta atgtcgtcaa acaacaacca acgcaataac ccgccgggag
    18421 gcccgccctc ccgcactgtc atcgaattcc cgttaactct tcatctgtta gtcactttta
    18481 attaaccaaa tcgtcacaat aatccgccac gatggagcca cttttttagg gaggctgcat
    18541 catgcaaacg attatccgtg tcgagaagct cgccaaaacc ttcaatcagc atcaggcgct
    18601 gcatgcggtt gatctgaaca ttcatcacgg tgaaatggtg gctctgcttg ggccgtcggg
    18661 ttccggcaaa tccacccttt tacgtcactt aagcggtttg attaccggcg ataaatccgc
    18721 cggcagccat atcgagctgc tgggccgcac agtccagcgc gaaggccgtc tggcgcgcga
    18781 tatccgcaaa agccgcgcca acaccggcta catcttccaa caattcaacc tggtgaaccg
    18841 cctgagcgta ctggagaacg tgctgattgg cgcactcggc agcacgccgt tctggcgcac
    18901 ctgttttagc tggtttaccc gcgagcagaa acaacgcgcg ttacaggcgc tgacccgcgt
    18961 tggcatggtg cattttgccc atcaacgcgt ttccaccctc tccggcggac agcagcagcg
    19021 tgtggcgatt gcccgcgcgc tgatgcagca ggcgaaggtg attctggccg atgaacccat
    19081 cgcctcgctg gacccggaat ccgccggcat cgtgatggac accctgcgcg acatcaatca
    19141 gaacgacggc atcaccgtgg tcgtcacgct gcatcaggtg gattacgccc tgcgctactg
    19201 cgaacgcatc gtcgccctgc gccaggggca cgttttctac gacggcagca gccaacagtt
    19261 tgataacgaa cgttttgacc atctctaccg cagcattaat cgcatcgaag agaacgcgaa
    19321 agctgcctga catccccatc attgaggaaa acgaatgaac gctaagataa ttgcctcgct
    19381 ggccttcacc agcatgttca gcctcagcac cctgttaagc ccggcacacg ccgaagagca
    19441 ggaaaaggcg ctgaatttcg gcattatttc aacggaatca cagcaaaacc tgaaaccgca
    19501 atggacgcca ttcttacagg atatggagaa gaagctgggc gtgaaggtga acgccttctt
    19561 tgccccagac tacgcaggca ttatccaggg aatgcgcttc aataaagtgg atatcgcctg
    19621 gtacggcaac ctgtcggcaa tggaagcggt ggatcgcgcc aacggccagg tcttcgccca
    19681 gacggtcgcg gcggatggat cgccaggtta ctggagcgtg ttgatcgtca acaaagatag
    19741 tccgatcaac aacctgaacg atctgctggc gaagcggaaa gatctcacct tcggcaatgg
    19801 cgatcctaac tccacctctg gcttcctcgt ccccggttac tacgtctttg ccaaaaacaa
    19861 tatctccgcc agcgacttta agcgcaccgt caacgccggg catgaaacca acgcgctggc
    19921 cgtcgccaac aagcaggtgg atgttgccac caacaacacc gaaaacctcg acaagctgaa
    19981 aacctccgcg ccagagaagc tgaaagaact gaaggtgatc tggaagtcgc cgctgatccc
    20041 aggcgatccg atcgtctggc gcaagaatct ctccgaaacc accaaagaca agatctacga
    20101 cttctttatg aactacggaa aaacgccgga agagaaagcg gtgctggaac gcctgggctg
    20161 ggcaccgttt cgcgcctcca gcgacctgca actggtgccg attcgccagc tcgcactgtt
    20221 taaagagatt cagggcgtaa aaagcaataa cggactgaat gagcaggaca agctggcgaa
    20281 aaccaccgag attcaggcgc agctggatga cctggaccgc ctgaacaacg cgttaagcgc
    20341 gatgagttcg gtgagtaaag cggtgcagta atgtgctcga tcggtcccct ctcccttgag
    20401 ggagagggtt agggtgaggg ggaaatgcgc acgctgatgc cctcaccccg gccctctccc
    20461 acagggagag ggagaaaaca ttaaacggag ccaaacatgc aaaccatcac catcgcccca
    20521 cccaagcgca gctggttctc gcttctgagc tgggccattg ttctcgccgt gttggtcgtc
    20581 tcgtggcagg gcgcggaaat ggccccgctt acgctgatca aagacggcgg caacatggcg
    20641 accttcgccg ccgacttctt cccgcccgat ttcagccagt ggcaggatta cctcaccgaa
    20701 atggccgtca cgctgcaaat cgccgtctgg ggcaccgcgc tggcagtggt tctctccatc
    20761 cccttcggcc tgatgagcgc cgaaaacctg gtgccgtggt gggtttacca gcccgttcgc
    20821 cgcctgatgg acgcctgccg cgccattaac gaaatggtct tcgccatgct gttcgtggtc
    20881 gccgtcggtc tcggaccgtt cgctggcgtg ctggcgctat ttattcacac caccggcgtg
    20941 ctctcgaaac tgctttccga agcggtggaa gcgattgaac ccggcccggt ggaaggcatt
    21001 cgcgccaccg gcgccaacaa gctggaagag atcctttacg gcgtgctgcc acaggtaatg
    21061 ccgctgctga tctcctactc cctctatcgc ttcgaatcca acgtccgctc ggcgaccgtc
    21121 gtcggcatgg tcggtgcagg cgggatcggc gtcaccctgt gggaagcgat tcgcggtttc
    21181 cagttccaac aaacctgcgc cctgatggtg cttatcatcg tcacggtcag cctgctggat
    21241 ttcctctctc aacggttgtg taagcacttt atctgataag tgaggcattg atatctatgc
    21301 acttgtctac acatccgacc agctacccaa cacgctatca ggagatagcc gcaaaacttg
    21361 agcaggagct tcgtcaacac tatcgctgcg gcgactatct tcccgccgag cagcaactgg
    21421 cggcgcgctt tgaggtaaac cgccacaccc tgcgccgcgc catcgaccaa ctggtggaaa
    21481 aaggctgggt acagcgccgt cagggcatcg gcgtgctggt gctgatgcgc ccgttcgatt
    21541 acccgctcaa cgcccaggcg cgttttagcc agaacctgct ggaccagggc agccatccca
    21601 ccagcgaaaa actgctgtcg gtattgcgcc ccgcgtccgg tcacgttgct gacgcgctgg
    21661 ggattaccga gggtgagaac gttatccatt tgcgcaccct gcgtcgcgtc aacggcgtcg
    21721 cgctctgttt aatcgaccac tactttgcgg acctcaccct ctggccgaca ctgcaacgtt
    21781 tcgacagcgg ttcgctgcac gattttctgc gcgagcaaac cggaattgcg ctgcgccgca
    21841 gccagacgcg gatcagcgcc cgccgcgccc aggccaaaga gtgccagcgc cttgaaatcc
    21901 cgaatatgtc gccgctgctg tgcgtgcgca cccttaacca ccgtgacggt gaaagcagcc
    21961 cggcggagta ctccgtcagc ctgacgcgcg ccgacatgat tgaattcact atggagcact
    22021 gaatgcacgc agataccgcg acccgccagc actggatgtc cgtactggcg cacagccaac
    22081 cggctgaact ggcagcccgc ctgaacgcgc tgaacatcac cgccgactat gaagtgatcc
    22141 gcactgccga aactggcctg gtgcagattc aggcgcgaat gggcggtacc ggcgaacgct
    22201 tttttgccgg cgacgccacg ctgacccgtg ccgccgtacg cctgactgac ggcaccctcg
    22261 gctacagctg ggtgctgggg cgtgataaac agcacgccga acgctgcgcg ctgattgatg
    22321 cgctgatgca gcaatctcgc cactttcaaa acttatcaga aacccttatt gccccgctgg
    22381 acgctgaccg tatggcacgc attgccgcac gccaggccga agtgaacgcc agccgggtcg
    22441 acttctttac gatggttcgc ggagacaacg catgaccctg gaaaccgctt ttatgcttcc
    22501 cgtgcaggat gcccagcaca gttttcgtcg cctgttaaag gccatgagcg agccgggcgt
    22561 gattgtcgcc ctgtatcagc tcaaacgcgg ctggcagccg ctgaatatcg ccaccaccag
    22621 cgtgctgctg acgctggccg ataacgacac gccggtgtgg cttgccacgc cattaagtaa
    22681 cgatatcgtc aatcagagcc tgcgttttca taccaacgcg ccgctggtca gccagccgga
    22741 acaggcgacc ttcgcggtga cggatgaggc gatttccagc gaacagctca acgccctttc
    22801 caccgacacc gccgttgcgc cggaagcggg cgcgacgctg attttacagg tcgccagcct
    22861 gagcggcgga cgcatgttgc gtctcaccgg cgcgggcatt gccgaagaac gaatgatcgc
    22921 tccgcagctg ccagagtgca ttctgcacga actcaccgag cgcccgcacc cgttcccgct
    22981 cggcatcgac ctaatcctga cctgcggcga gcgcctgctg gctatcccgc gaaccactca
    23041 tgtggaggtg tgctgatgta cgttgccgtg aaagggggcg aaaaggcgat cgacgccgcc
    23101 cacgccctgc aagagagccg acgccggggc gataccgatt tgcccgaact gagcgtcgcc
    23161 cagattgaac agcagcttaa cctcgcggtg gatcgcgtga tgacagaagg cggcattgcc
    23221 gaccgcgaac tggcggcgct ggcgctgaaa caggccagcg gcgataacgt cgaagcgatt
    23281 ttcctgctac gcgcctaccg caccacgctg acgaaactgg cggtaagcga gccgctcgac
    23341 accacggaga tgcgtctcga acggcgtatt tcagcggttt ataaagatat tcccggcggc
    23401 cagctgcttg gcccaaccta cgactacacc catcgcctgc tcgatttcac tctgctggca
    23461 aacggcgaag cgccgacgct gaccaccgcc gacagcgaac aacagccgtc gccgcacgtt
    23521 ttcagcctgc tggcgcgtca ggggctggcg aagtttgaag aggatagcgg agcacagccg
    23581 gatgacatca cccgcacgcc gccggtttac ccctgttcac gttcttcccg tttgcagcag
    23641 ttaatgcgcg gcgacgaagg ctatttgctg gcgctggctt actccaccca gcgcggttac
    23701 gggcgcaacc acccgttcgc gggcgagatc cgcagtggtt acatcgacgt gtcgattgtg
    23761 ccggaagagc tgggatttgc ggtaaacgtc ggcgaactgc tgatgaccga gtgtgaaatg
    23821 gtcaacggtt ttatcgaccc gccggatgag ccgccgcact tcacgcgcgg ctacgggctg
    23881 gtattcggca tgagcgagcg caaagcgatg gcaatggcgc tggttgaccg tgcgttgcaa
    23941 gccccggaat acggcgagca cgcgacaggc ccggcgcagg atgaagagtt cgtgctggcg
    24001 catgccgaca acgtcgaagc cgcaggcttt gtctcgcacc tcaaactccc gcactacgtc
    24061 gatttccagg ccgaactgga gctactcaaa cgtctgcaac aggagcagaa ccatggctaa
    24121 tctgagcggc tacaactttg cctacctcga cgagcagacc aaacgcatga tccgccgcgc
    24181 catcttaaaa gcggtggcga tccccggtta tcaggtaccg tttggcgggc gcgagatgcc
    24241 gatgccgtac ggctggggaa ctggtggtat ccagctcacc gccagcgtga ttggcgaaag
    24301 cgacgtgttg aaggtgattg accagggtgc ggatgacacc accaacgccg tgtcgattcg
    24361 caacttcttt aagcgcgtga ccggggtaaa caccactgaa tgtacggacg atgcgacgct
    24421 tatccagacg cgtcaccgca tccccgaaac gccgctgacc gaagatcaga tcattatctt
    24481 ccaggtgcca atcccggaac cgctgcgctt tatcgagccg cgcgaaacgg agacccgcac
    24541 tatgcacgcg ctggaggagt acggcgtgat gcaggtgaaa ctgtatgaag atatcgcccg
    24601 cttcggtcat atcgccacca cctatgctta tccggtgaag gtgaacgggc gctacgtgat
    24661 ggacccgtcg ccgatcccga aattcgataa cccgaaaatg gacatgatgc ccgccctgca
    24721 gctgttcggc gcagggcgcg agaagcgcat ctatgcggtg ccgccgttta cccgcgtgga
    24781 aagtctcgat ttcgacgatc acccgttcac cgttcagcag tgggatgagc catgcgccat
    24841 ctgcggatcg acccacagct atcttgatga agtggtgctg gatgacgccg gaaaccgcat
    24901 gtttgtctgc tccgataccg attattgccg ccaacagagc gaggcgaaaa gccaatgaat
    24961 caaccgttac tttcggtcaa taacctgacc cacctttacg cgccgggcaa aggctttagc
    25021 gatgtctctt ttgatttatg gccgggggaa gtgctgggca ttgtcgggga atccggctcc
    25081 gggaagacca cgctgctgaa gtcgatctcc gcgcgtctga cgccgcagca gggggagatt
    25141 cactatgaaa accgttccct ctatgcaatg agcgaggccg accgccgtcg cctgctgcgc
    25201 accgaatggg gcgtggtgca tcagcatccg ctcgacggcc tgcgccgcca ggtgtcggca
    25261 ggcggcaata tcggcgagcg gctgatggcg accggggcgc gacattacgg cgatattcgc
    25321 gctaccgcgc agaagtggct ggaagaggtg gagattcccg ccaaccgtat cgacgacctg
    25381 ccgaccacct tttccggcgg tatgcagcag cgtttgcaga ttgcccgcaa cctggtgacg
    25441 catccgaagc tggtgtttat ggatgaaccg accggcgggc tggatgtgtc ggtgcaggcc
    25501 cgcctgctcg acctgctgcg cggactggtg gtggagctga acctcgcggt agtgattgtc
    25561 acccacgatt taggcgtcgc ccgcctgctg gcggaccgtt tgctggtgat gaagcagggg
    25621 caagtggtgg agagtgggtt aaccgaccgc gtgctcgacg acccgcatca tccgtataca
    25681 cagctgctgg tgtcatcggt tttgcagaat tgagccggtg ccggatgcgg cgtaaacgcc
    25741 ttatccggcc tacaaatgcg ctccccgtag gtcggataag acgcgtcagc gtcgcatccg
    25801 acacccgaac cacgaggcga aaaatgatta acgtacaaaa cgtcagtaaa accttcatcc
    25861 tgcaccagca aaacggcgtg cgcctgcccg tcctcaaccg cgcctcgctc atcgtcaacg
    25921 cgggcgaatg cgtggtgctc cacggccatt ccggcagcgg aaaatcaacc ctgctacgct
    25981 cgctgtacgc caactatctg cccgacgaag ggcaaatcca gatcaaacac ggtgacgaat
    26041 gggtcgatct ggtcaccgcg ccagcgcgca aagtggtgga gatccgcaaa accaccgttg
    26101 gctgggtgag ccagtttctg cgcgtcatcc cgcgtatttc ggcgctggaa gtggtgatgc
    26161 agccgctact cgataccggc gtaccgcgtg aagcctgcgc cgctaaagcc gcgcgtctgc
    26221 tcacccgcct gaacgtgccg gaacgcctgt ggcatctggc gccatcgacc ttctccggcg
    26281 gcgaacagca gcgcgtcaac atcgcccgcg gctttatcgt cgactacccc attctgctgc
    26341 tcgacgaacc taccgcctcg ctggacgcca aaaacagcgc cgccgtagtc gagctgattc
    26401 acgaagccaa agcccgtggc gcggccatcg tcggcatctt ccacgacgaa gccgtacgta
    26461 atgacgttgc cgaccgcctg cacccaatgg aagcctcttc atgattatca ataacgtcaa
    26521 attggtgctg gaaaacgaag tggtacacgg ttcactggag gtgcaggacg gcgaaatccg
    26581 cgcctttgcc gaaagccaga gccgcctgcc ggaggcgatg gacggcgaag gcggctggct
    26641 gctgccgggg ctgattgagc tgcataccga taatctggat aaattcttca ccccgcgccc
    26701 gaaagttgac tggcctgccc actcggcgat gagcagccac gacgcgctga tggtggcgag
    26761 cggcatcacc accgtactgg atgccgtggc aattggcgac gtgcgcgacg gcggcgatcg
    26821 gctggtgaat ctggagaaga tgatcaacgc catcgaagag acgcagaaac gcggcgtcaa
    26881 ccgcgccgag caccgtctgc atctgcgctg cgaactgccg catcacacca cgctgccgct
    26941 gtttgaaaaa ctggtgcagc gcgagccggt gacgctggtg tcgctgatgg accactcgcc
    27001 gggccagcgc cagttcgcca atcgcgagaa gtatcgcgaa tattatcagg gcaaatactc
    27061 cctcactgat gcgcagatgc agcagtacga agaagagcaa ctggcgctcg ccgcacgctg
    27121 gtcgcagccg aatcgcgaat ccatcgccgc cctgtgccgc gcgcgaaaaa ttgcgcttgc
    27181 cagccacgat gacgccaccc acgcccacgt tgctgaatct caccagcttg gcagcgtgat
    27241 cgccgaattt cccaccacgt tcgaagcggc ggaagcctcg cgcaagcatg gcatgaacgt
    27301 gctgatgggc gcgccgaata ttgtgcgcgg cggctcgcac tccggcaacg tggcagccag
    27361 tgaactggcg cagcttggcc tgctggatat cctctcttcc gactactacc ccgccagcct
    27421 gctcgatgcg gcatttcgcg tcgccgatga cgagagcaac cgctttacgc tgccgcaggc
    27481 ggtgaagctg gtgactaaaa atccagcgca ggcgcttaat ctccaggatc gcggggtgat
    27541 tggcgagggc aaacgcgcgg acttggtgct ggcgcatcgc aagggcaatc acattcatat
    27601 cgaccacgtc tggcgtcagg gtaaaagggt gttctgatga tgggaaaact gatttggtta
    27661 atggggccgt ccggctccgg gaaagatagc ctgctggcgg aactccgcct gcgggaacaa
    27721 actcagctac tggtggcgca tcgctacatc acgcgcgacg ccagcgccgg aagcgaaaac
    27781 catatcgctc tgagcgagca ggagtttttt acccgcgcgg ggcagaacct actggcctta
    27841 agctggcacg ctaacggtct gtattatggc gtcggcgtcg agatcgacct ctggctgcac
    27901 gccggattcg acgtgctggt caacggctca cgcgcccatc tgccgcaggc gcgggcgcgc
    27961 tatcaatcgg cgctgctgcc cgtctgttta caggtttcgc cggagatcct ccgccagcgc
    28021 ctggaaaacc gtggccgtga aaacgccagt gaaattaacg cccgcctggc gcgcgccgcc
    28081 cgctatactc cacaggattg ccatacgctc aacaatgacg gcagcctgcg ccagtcggtc
    28141 gacacgctgc tgacgctgat tcatcagaag gagaaacacc atgcctgctt gtgagcttcg
    28201 cccggccacg cagtatgata ccgacgcggt ttacgcgctg atttgtgagc taaaacaggc
    28261 ggagtttgac caccacgcat ttcgcgtggg ctttagcgcc aatctgcgcg acccgaacat
    28321 gcgctatcat ctggcgctgc tggatggcga ggttgtcggc atgatcggcc tgcatttgca
    28381 gtttcatctg catcatgtca actggatcgg cgaaattcag gaactggtag tgatgccgca
    28441 ggcgcgcggc ctgaacgtcg gtagcaagtt gctggcgtgg gcagaagaag aagcccgcca
    28501 ggccggggcc gaaatgaccg agctttcgac caacgtgaag cgccacgacg cgcaccgttt
    28561 ctatctgcga gaaggctacg agcagagtca cttccgcttc accaaggcgc tgtaacatga
    28621 gtctgaccct cacgctcacc ggcaccggcg gcgcacaggg cgttccggca tggggctgcg
    28681 agtgcgcggc ctgcgaccga gcgcggcgct cgccgcagta tcgccgccaa ccgtgcagcg
    28741 gcgtagtgaa gtttaacgag gcgatcaccc tgatcgacgc cgggctgcac gatctcaccg
    28801 atcgctggtc tcccggatcg ttccagcagt ttttgctgac gcattatcat atggatcacg
    28861 tccaggggct gtttccgctg cgctggggcg tgggcgatcc gatcccgatc tacggcccgc
    28921 cggatgaaca gggctgcgac gatctgttta aacatcccgg gctgcttgat ttcagccaca
    28981 cggtggaacc gtttgtggtg tttgatttgc aggggttgca ggtcacgccg ctgccgctta
    29041 accactcaaa actcactttc ggctatttac tggaaacggc gcacagccgg gtggcgtggc
    29101 tgtctgacac cgctggcttg ccggaaaaaa cgctgaaatt tttacgcaat aatcagccgc
    29161 aggtaatggt gatggattgc agtcacccgc cgcgcgcgga tgcaccgcgt aatcactgtg
    29221 atttaaatac cgtgcttgcg ctgaatcagg ttatccgctc gccacgggtg attctgaccc
    29281 atatcagcca ccagtttgat gcgtggctga tggaaaacgc actaccgtca gggtttgagg
    29341 tggggtttga tgggatggag attggggtgg cgtgatgaga gggaatgtgc gcgctggccc
    29401 cctcacccta accctctccc cagaggggcg aggggaccga ttgtgctcga tattgaatat
    29461 tgcgctcgtt ttctccctct ccccattggg gtgaggggcg atgcctgctc catacccaac
    29521 ctcatcgccc atactcatct tccattctcc gctcttcatc ctccagttgc cgacgctcct
    29581 gatcaagctg gcgctggcga tcgtccagct gcctgcggcg atcttcaaac tggcggcggc
    29641 ggtcgtcata ttgtctgcgc cgatcgtcgc tcacttcacg ctgccagccg tcgtcgcgcg
    29701 aatcttcata gtctcgccca cggtcagggt tataagcgtc attaatcgcc tgctgaatat
    29761 taccaatagt atcgtcgata atatcagcct gggccggaac ggtggacagc gtgagcaggg
    29821 tgaataaaag aaatagcgga aagcgtttca ttagccaacc tcaaaaagaa actctatcca
    29881 cattagtcat tactcatcca tgcaagtagt ggatgaatct caatttctcc gctgctctat
    29941 tgccgtaatc gcctccacgc gttgttgatg acgaccgcct tcgtactgtg cgcccagcca
    30001 cgcatccaca atcatttttg ccagttcgag gccaaccact cgtgaaccaa aagccagtgc
    30061 aacttcactg gcataatgcg gataatcagt gcgctctgac gacctggtgt ctttatcaat
    30121 cacctcactc attgaccatg aataaaaggc tggcacagca acgcagaatc ctgcggtgcc
    30181 agcgacatga ccgcccattg ctccggtacg ttttttaaaa agaaacaccg gttgcattga
    30241 taaattgtgc tcagtatttg ccgaagtaca atatatataa taaatatata aaacacctca
    30301 tattatttac agagataata aattcgcgta attttccctc ttcagcacaa atgcctaata
    30361 aaatggatcg ttcagctcac acttccactt attaaagtcg caaattccca attatgataa
    30421 ccccagcttt aaatgacgta ttttgcatgg attacatatt tacgacataa atgataaatg
    30481 gagatttaca tgaataactc tcggttattc cgtttgagca gaattgttat tgcgttaact
    30541 gccgccagcg gcatgatggt aaataccgct aacgcgaaag aggaagcgaa agccgccact
    30601 caatataccc aacaggttaa tcagaattac gccaaatcat taccgtttag cgatcgtcag
    30661 gattttgacg acgctcagcg tggatttatc gctccgctgc tggatgaagg tattctgcgc
    30721 gatgctaatg gcaaaccata ttatcgcggg gaagattata aatttgatat caatgcgccc
    30781 gcgcccgaaa ccgttaaccc tagcctgtgg cgtcagtcgc agttaaacgg tatttccggc
    30841 ctgtttaaag tcaccgacag aatgtaccag gttcgcggtc aggatatctc caacatcacc
    30901 tttatcgaag gtgataccgg gattattgtc atcgacccgc tggtgacgcc accgagcgca
    30961 aaagccgccc ttgaccttta cttccaaaat cgcccgcaaa aaccgattgt tgcggttatt
    31021 tatacccaca gccataccga ccactatggc ggcgtgaaag gcattatctc tgaagctgat
    31081 gtgaaatccg gtaaagtgca ggttatcgcc cctgctggct ttatggacga agccatcagc
    31141 gaaaacgtac tggcgggtaa tattatgagc cgccgtgcac tttactccta cggcctgctg
    31201 ctggcgcata accctcaggg taacatcggc aatggtcttg gcgtaacgct ggcatcgggc
    31261 tacccgagca tcatcgcacc gaacaaaacc atcaccaaaa ccggtgagaa gatgattatc
    31321 gacggcctgg agtttgactt cctgatgacc ccaggtagcg aagcaccagc cgaaatgcac
    31381 ttctatattc cggccctgaa agcgctgtgt accgccgaga acgccacgca taccctgcac
    31441 aacttctaca ctctgcgcgg cgcgaaaacc cgcgacacca gcaagtggac cgagtatctg
    31501 aacgaaacgc tggatatgtg gggtaacgac gcggaagtcc tgtttatgcc gcacacctgg
    31561 ccggtctggg gcaataagca tatcaatgat tatattggta aatatcgcga tactatcaag
    31621 tacattcacg accagaccct gcacctggcg aaccagggct acaccatgaa tgaaatcggc
    31681 gacatgatta aactgccgcc tgcacttgcc aataactggg ccagccgtgg ctattacggt
    31741 tctgtcagcc acaacgcccg cgcggtgtat aacttctatc ttggctatta cgacggtaac
    31801 ccggctaacc tgcatccgta tggtcaggtg gagatgggta aacgttacgt gcaggcgctg
    31861 ggcggttctg cccgtgtcat caacctggcg caagaagcga acaagcaagg tgattaccgc
    31921 tggtcggcag aactgctgaa acaggtgatt gccgccaacc cgggtgacca ggtcgcgaag
    31981 aatctgcaag cgaataactt tgaacagctg ggctatcagg ccgagtccgc cacctggcgc
    32041 ggtttctacc tgaccggcgc gaaagagctg cgcgaagggg tgcataagtt cagccacggc
    32101 accatcggtt ccccggacac cattcgcggg atgtcggtcg aaatgctgtt cgactttatg
    32161 tccgttcgcc tcgatagcgc gaaagccgcg ggtaaaaata tcagcctgaa cttcaatatg
    32221 agcaatggcg ataacctcaa cctgacgctg aacgatagcg tgcttaacta ccgtaaaaca
    32281 ctgcaatccc aagctgacgc ctctttctac atcagccgtg aagatctgca cgccgtgctg
    32341 accgggcaag ccaaaatggc ggatctggta aaagcgaaga aagccaaaat tattggcaat
    32401 ggcgcgaaac tggaagaaat tatcgcctgt ctggataatt tcgatttgtg ggtgaatatc
    32461 gtaaccccaa attaatgtca ctcgcttttg ggggcgctga agcccccaaa agcatttatt
    32521 ttaaaaatat atacagccaa ttaataaata aaatatataa gttatgaatg cggtgactga
    32581 ataaagctat ttttattcat cgagaaaata gccatactga cgccttcaat ggatattgat
    32641 attcactaca gatattgttc gcagcagaca ggatgtattt aatatgccaa cagttctctc
    32701 tcgcatggcg atgcaactca aaaaaacagc ctggataatt cccgtcttca tggtttcggg
    32761 atgctcatta tctccggcaa tcccggtgat cggtgcttat tatcccagct ggtttttctg
    32821 cgccattgcc agccttattt tgatgctcat cacgaggcga gttattcagc gggcaaatat
    32881 caatctggca tttgtcggaa ttatttatac cgcccttttt gctctctacg ccatgctgtt
    32941 ctggctggca tttttctaat ctatttgaga cgcgctcatg gaaagtacgc cgaaaaaagc
    33001 tcctcgcagt aaattccctg ctctgttagt ggttgcattg gcgctggttg cccttgtttt
    33061 cgttatctgg cgcgtagaca gtgcgccatc aactaatgac gcttacgcgt cagcagatac
    33121 cattgatgtg gtgccggaag tcagcggccg cattgtagaa ctggcggtca ccgacaacca
    33181 ggcagtcaaa cagggcgatt tgctgttccg cattgacccg cgcccgtacg aagccaatct
    33241 ggcgaaagct gaagcctccc tcgcggcgct ggataagcaa attatgctca cccagcgtag
    33301 cgttgatgcg caacagtttg gtgccgactc gattaatgcc acggtagaaa aagcccgtgc
    33361 cgccgcgaaa caggccacag atacattacg ccgtaccgag ccattactga aagagggttt
    33421 tgtctcagcg gaagacgttg accgtgcaag aactgcgcag cgcgccgcag aagccgatct
    33481 taatgccgta ttgttacagg cgcagtcagc cgccagcgcc gtcagcggcg tcgatgcgct
    33541 ggtggcgcaa cgtgcggcgg tcgaagcgga tattgccctg accaaactgc atctggaaat
    33601 ggcgaccgtt cgcgcgccgt ttgatggccg ggtcatttcc ctcaaaacct ccgtcgggca
    33661 atttgcttct gccatgcgcc ctatttttac cctaatcgac actcgtcact ggtacgtgat
    33721 cgccaacttc cgcgaaaccg atctgaaaaa tattcgctca ggtacacccg caacgattcg
    33781 cctgatgagt gacagcggca aaaccttcga gggtaaagtg gattcgattg gctacggcgt
    33841 gctaccggat gacggcggcc tggtgctggg cggcctgccg aaagtttctc gctctattaa
    33901 ctgggtccgc gttgcccagc gttttccggt caaaatcatg gtcgataaac ctgacccgga
    33961 aatgttccgc atcggcgctt cggcagtcgc taatcttgag ccgcaataat gagcgcgctc
    34021 aactccctgc cattaccgat ggtcaggctg ctggcgttct ttcatgaaga gttaagcgag
    34081 cggcgacccg gccgcgtgcc gcagatcgtg caactctggg taggctgcct gctggtgatt
    34141 ctgatctcga tgacttttga gatccctttt gtggcgttat cgctggcagt gctgttttac
    34201 ggtattcagt cgaacgcgtt ttacaccaaa tttgtggcga tcttgtttgt ggttgccacg
    34261 gtgctggaga tcggcagcct gtttttgatc tacaaatggt catacggcga accgttgatc
    34321 cgattgatca tcgccgggcc gatcctgatg ggccgcatgt ttttgatgcg cacccatcgg
    34381 ctgggactgg tctttttcgc tgtcgccatt gtcgccattt acgggcaaac cttccccgcc
    34441 atgctcgact atccggaagt ggtcgtgcgc ttaacgctgt ggtgtatcgt tgttggcctc
    34501 tatccaacct tgctgatgac gttaatcggc gtgctgtggt ttcccagtcg tgccatttcg
    34561 caaatgcatc aggcgcttaa tgatcggctt gatgatgcca ttagtcacct gacagacagc
    34621 ctcgcaccgc tacccgaaac gcggattgaa agagaggcgc tggcgctgca aaaactgaat
    34681 gtcttttgtc tcgcggacga tgccaactgg cgaactcaaa gcgcatggtg gcaaagctgc
    34741 gtggcaacgg taacctacat ttactcgacg ctgaatcgct acgatcccac ctcttttgct
    34801 gattctcagg caattattga atttcggcaa aaattagcct cagaaatcaa caagctgcag
    34861 catgccgttg ctgaaggtca gtgctggcaa agcgactggc ggatcagtga aagtgaagcg
    34921 gtggcggcac gggaatgtaa cctggagaat atctgccaga cgttgttaca actgggccag
    34981 atgaacccga atacgccgcc aacgcccgcc gccaaaccgc catcaatggt cgccgatgct
    35041 tttaccaatc cagactatat acgctacgcg gtaaaaacat tgctcgcctg tttgatctgt
    35101 tacacctttt acagcggcgt ggactgggaa ggcattcaca cctgtatgct gacctgcgtg
    35161 atcgtctcta atccaaatgt cggttcgtcg taccagaaga tggtgctgcg ttttggcggg
    35221 gccttttgcg gcgcgattct ggcgctgtta ttcacgctac tggtcatgcc ctggctggac
    35281 aatattgtcg aattgctgtt tgtgctggca ccgattttcc tgttgggcgc atggattgcc
    35341 accagctctg aacgctcttc ttatatcggc acacagatgg tggtcacctt cgcgctcgcc
    35401 acgctcgaaa acgtttttgg cccagtgtac gacctggtgg aaattcgcga tcgcgccctg
    35461 ggtatcatca ttggtaccgt ggtgtccgcg gtgatttaca cctttgtctg gcctgaaagt
    35521 gaagcgcgca cgctgccgca aaaactggct ggcgcgctgg gtatgttaag taaagtaatg
    35581 cggatcccac gccagcagga agtcacggct ctgcgcactt atctgcaaat tcgtatcggt
    35641 ctgcatgcgg cgtttaatgc ctgtgaagag atgtgccaac gcgtggcgct ggagcgtcaa
    35701 ctggacagcg aagaacgcgc cttactgatt gaacgttcgc aaacggttat tcgtcagggc
    35761 cgcgatatcc ttcacgcctg ggatgccacc tggaattcgg cgcaggcgct ggataacgca
    35821 ctacagccgg acagagcagg tcagtttgcc gacgccctgg aaaaatacgc tgccggtctg
    35881 gcaaccgcac tcagccgttc tcctcaaata acgcttgaag agacacccgc ctcgcaggcc
    35941 atcctgccca ccttattaaa acaggagcaa cacgtctgcc agcttttcgc ccgcttgcca
    36001 gactggacag ccccggcatt aacgcccgcc acggaacagg cacaaggagc cacgcaatga
    36061 tcaatcgtca actttcacgt ctgctgttgt gcagcattct cggcagcacg acgctgattt
    36121 ccggctgtgc cttggtacgc aaggattctg cgcctcatca acagctcaaa ccggaacaaa
    36181 tcaaactggc cgatgatatt catcttgcca gctccggctg gccgcaggcg cagtggtgga
    36241 aacaactcaa tgacccgtag ctggatgcgc tgatccaacg gacgctaagt ggttcacaca
    36301 ccctcgccga agcgaaactg cgggaagaaa aagcgcagtc ccaggccgat ttgttagatg
    36361 ccggttcaca attacaggtc gcagcgttag ggatgctcaa ccgccaacgc gtctcggcga
    36421 acggcttttt aagcccttat gcgatggatg cgcccgcact gggtatggac gggccgtact
    36481 atacggaagc cacagtaggt ttgtttgccg gactggatct tgatttgtgg ggtgtgcatc
    36541 gctcagcggt tgccgccgcc attggcgcgc ataatgccgc gctggcagaa accgcagcag
    36601 tagagctatc gctgaccacg ggcgtagcgc agctttatta cagtatgcag gccagctatc
    36661 agatgctcga tctgttagaa caaactcgcg atgtgattga ttacgcggtg aaagcgcacc
    36721 agagtaaagt ggcgcacggt ctggaagcac aagtgccttt ccacggcgcg cgggcacaga
    36781 ttctggcggt cgataaacaa attgctgccg tcaaagggca aatcaccgaa acgcgagaat
    36841 ctctgcgtgc attgattggc gcgggggcca gcgatatgcc ggagatcaaa ccggtggcat
    36901 taccacgagt ccagaccggc attccggcga cactctctta tgagttgctc gccagacgcc
    36961 cggatctgca agccatgcgc tggtatgttc aggcgtcatt agatcaggtg gattccgcgc
    37021 gggcgctgtt ctacccgagc tttgatatca aagcgttttt cggccttgat gccatccacc
    37081 tggatacctt attcaaaaaa accagtcgcc agttcaactt catcccgggt ctgaaattgc
    37141 cgctgtttga cggtggacgg ttgaatgcca atctcgaagg cacgcgcgcc gccagcaaca
    37201 tgatgattga acgttacaac cagtcagtac tgaacgcggt gcgtgacgtt gccgtcaacg
    37261 gcacgcgtct gcaaacgctc aacgacgagc gagaaatgca ggctgaacgc gtggaagcaa
    37321 cgcgcttcac ccagcgcgct gccgaggccg cctatcagcg cggcttaacc agccgcttac
    37381 aggccactga agcccggttg ccagtacttg ccgaagagat gtcattactg atgctggaca
    37441 gccgccgggt gatccaaagc attcagttga tgaaatcgct gggcggcggg tatcaggcgg
    37501 ctcccgtcgt cgagaaaaaa taaaatgtct gccgcgtgat ggctgtcacg cggtatttcg
    37561 tttcgtcacg tcaaaactga cgacagcctg tttttcgtca gagttttgaa taaatagtgc
    37621 ccgtaatatc agggaatgac cccaaataaa atgtggcata aaagatgcat actgtagtcg
    37681 agagcgcgta tgcgtgattt gattaactgg agcgagaccg atgaaaaaag tcgtcacggt
    37741 ttgcccctat tgcgcatcag gttgcaaaat caacctggtc gtcgataacg gcaaaatcgt
    37801 ccgggcggag gcagcgcagg ggaaaaccaa ccagggtacc ctgtgtctga agggttatta
    37861 tggctgggat ttcattaacg atacccagat cctgaccccg cgcctgaaaa cccccatgat
    37921 ccgtcgccag cgtggcggca aactcgaacc tgtttcctgg gatgaggcac tgaattacgt
    37981 tgccgagcgc ctgagcgcca tcaaagagaa gtacggtccg gatgccatcc agacgaccgg
    38041 ctcctcgcgt ggtacgggta acgaaaccaa ctatgtaatg caaaaatttg cgcgcgccgt
    38101 tattggtacc aataacgttg actgctgcgc tcgtgtctga cacggcccat cggttgcagg
    38161 tctgcaccaa tcggtcggta atggcgcaat gagcaatgct attaacgaaa ttgataatac
    38221 cgatttagtg ttcgttttcg ggtacaaccc ggctgattcc cacccaatcg tggcgaatca
    38281 cgtaattaac gctaaacgta acggggcgaa aattatcgtc tgcgatccgc gcaaaattga
    38341 aaccgcgcgc attgctgaca tgcacattgc actgaaaaac ggctcgaaca tcgcgctgtt
    38401 gaatgcgatg gaccatgtca ttattgaaga aaatctgtac gacaaagcgt tcgtcgcttc
    38461 ccgtacagaa ggctttgaag agtatcgtaa aatcgttgaa ggctacacgc cggagtcggt
    38521 tgaagatatc accggcgtca gcgccagtga gattcgtcag gcggcacgga tgtatgccca
    38581 ggcgaaaagc gccgccatcc tgtggggcat gggtgtaacc cagttctacc agggcgtgga
    38641 aaccgtgcgt tctctgacca gcctcgcgat gctgactggt aacctcggta agccgcatgc
    38701 gggtgtaaac ccggttcgtg gtcagaacaa cgtgcagggt gcctgcgata tgggcgcgct
    38761 gccggatacg tatccgggat accagtacgt gaaagatccg gctaaccgcg agaaattcgc
    38821 caaagcctgg ggcgtggaaa gcctgcctgc tcataccggt tatcgcatca gcgagctgcc
    38881 gcaccgcgca gcgcatggcg aagtgcgtgc cgcgtacatt atgggcgaag atccgctgca
    38941 aactgacgcg gagctgtcgg cagtacgtaa agcctttgaa gatctggaac tggttatcgt
    39001 tcaggacatc tttatgacca aaaccgcgtc ggcggcggat gtcattttgc cgtcaacgtc
    39061 gtggggcgag catgaaggcg tgtttactgc ggctgaccgt ggcttccagc gtttcttcaa
    39121 agcagttgaa ccgaaatggg atctgaaaac ggactggcaa atcatcagtg aaatcgccac
    39181 ccgtatgggt tatccgatgc actacaacaa cacccaggag atctgggatg agttgcgtca
    39241 tctgtgcccg gatttctacg gtgcgactta cgagaaaatg ggcgaactgg gcttcattca
    39301 gtggccttgc cgcgatactt cagatgccga tcaggggact tcttatctgt ttaaagagaa
    39361 gtttgatacc ccgaacggtc tggcgcagtt cttcacctgc gactgggtag cgccaatcga
    39421 caaactcacc gacgagtacc cgatggtact gtcaacggtg cgtgaagttg gtcactactc
    39481 ttgccgttcg atgaccggta actgtgcggc actggcggcg ctggctgatg aacctggcta
    39541 cgcacaaatc aataccgaag acgccaaacg tctgggtatt gaagatgagg cattggtttg
    39601 ggtgcactcg cgtaaaggca aaattatcac ccgtgcgcag gtcagcgatc gtccgaacaa
    39661 aggggcgatt tacatgacct accagtggtg gattggtgcc tgtaacgagc tggttaccga
    39721 aaacttaagc ccgattacga aaacgccgga atacaaatac tgcgccgtgc gcgtcgagcc
    39781 gatcgccgat cagcgcgccg ccgagcagta cgtgattgac gagtacaaca agttgaaaac
    39841 tcgcctgcgc gaagcggcac tggcgtaata cagtcctttc tacagcctcc tttcggaggc
    39901 tgttttttta tccattcgaa ctctttatac tggttactcc ctacccaatc gtattatcaa
    39961 aatgaaaaaa attatcgcat tgatgttgtt tttgacattc tttgcccacg ccaacgactc
    40021 cgagcctggc agccagtatt taaaggcagc agaggccggg gaccgacgcg cacaatattt
    40081 tcttgccgac agctggttta gctccggcga tttgagcaaa gccgaatatt gggcacagaa
    40141 agccgccgac agcggtgatg ctgatgcctg cgcgctgctg gcgcagatca aaatcaccaa
    40201 tccggtcagt ctggactatc cacaagcaaa agttcttgca gagaaagcgg cgcaagcggg
    40261 cagtaaagaa ggtgaagtaa cgctggcgca tattctggta aatactcaag cgggtaaacc
    40321 ggattatcca aaggctattt cgctgttaga aaacgcctcg gaagatctgg agaacgactc
    40381 tgccgtcgat gcccaaatgc tgcttggttt gatttacgcc aacggcgtgg gcattaaggc
    40441 cgacgatgac aaggcaacct ggtatttcaa acgcagctct gcaatttccc gaaccggtta
    40501 ttccgagtac tgggcgggaa tgatgttctt aaacggtgaa gaaggtttta tcgagaagaa
    40561 caagcaaaag gcgctgcact ggttgaacct gagctgtatg gaagggtttg ataccgggtg
    40621 tgaagagttt gaaaaattaa ccaacggtta aggtaggcct gataagacgc ggcaagcgtc
    40681 gcatcaggca ttcacacatt gccgggtgga tatccccggc aatcttcaat tattgatccg
    40741 cagttttatc aaatttgccc agcacttcac gctcataagc cagcgccttt ttgcggtcaa
    40801 atttgtgttc ccacttggca atcaccagca ccgccagcgc attacccacc acgttcagcg
    40861 cagtacgcgc catgtcgagg atacggtcaa caccagcaat aaacgccaga ccttccagcg
    40921 gaatacctac gctacccagc gttgccagca gcaccacaaa cgacacgccc ggcacgccag
    40981 caatcccttt cgaggtcacc atcagcgtca gcaccagaat gatttcctgc cagatggaca
    41041 ggtcaatgcc atacagctga gcgatgaaaa tagcggcaat actttgatac agcgtcgaac
    41101 catcaaggtt aaaagagtaa ccggtcggca ccacgaagct ggtgatcgac gccggtgctc
    41161 cgtaggcttc catcttctca ataattcgcg gcagtacgct ttcagagctg gcagtggagt
    41221 acgccagaat cagctcatct ttcagaatac gaatcaggat ccagacgctt aacccgcaca
    41281 ggcgcgccac aattcccagc actaccagcg cgaagaacag aatggcgaaa tgcaccagca
    41341 gcaccagttt cgccagtggc cacagagaag agaaaccaaa gttagccacc gtcaccgcaa
    41401 tcagcgcaaa cacacccacc ggtgcataac gcatcaccat gtgagtcact ttaaacatgg
    41461 tttcagagat ggagcggaac acggtcacca gcagttcacg atgcgtcgcg ggcagggaag
    41521 aaagccccag accaaacagc accgagaaaa agatgatcgg cagcatttcg cctttcgcca
    41581 tcgacgccac aatgttcgtc ggcaccagcg acaaaatcgt gcccataatg ccgtgggaac
    41641 tgctttgtac cgcttccgta gtgctctgat atttcgagat atcgacggtc gccagctgcg
    41701 acatatccac cccggcaccg ggctggaaga cgttcgccag agtgatcccc aaaatgatgg
    41761 cgacggtggt gatcacctcg aagtaaataa tggttttcgc gccaatacgc ccgagctgtt
    41821 tggcatcacc aacacccgcg atacccacca ccagcgtgga gatcacaatc ggcacaacaa
    41881 tcattttgat cagatggatg aagatatcac ccgccggaga gagcaaattg acgaccagcc
    41941 agtcgcggct gtcgctatgg tagtgcaggt agcttcccag gagaatgccc agcaccatag
    42001 caaacagaat ctgccaggcc aggctgaatt ttatattttt catcagaata acttcctcaa
    42061 tggagcgccg ttccccgttc agaaacgcga gaacgtgaca tactgccagg gcatgaattg
    42121 tgttgcgttg gtttgtagat ttttttaacg cttatgatca gtatcatttg cgcggtatgt
    42181 tgcgcaaccc tttaacaaca gagcttttcg ctgcattaat gcataataac gcgagtttat
    42241 ggcaggatta atgaataacc atttgttatg gaaagatttt tatttgttta atctgcaata
    42301 atactgatta gtgattattt ttggcatatg ttcattcgat tattttttat gcagctcaat
    42361 gacataatct tcatcaccag atgattaatc cgatctccgc tgcaacagtt ttgccatatt
    42421 aatcgactcg accagctgtg tccggttgat ccggggtgag ttgagatcca tcactttttg
    42481 ccatagttcg atggcttgcg cgtaattcgc ctgcataaac gcatcggaag ccagcagcat
    42541 cagagcggtg atttcgttag agtccagcgc gagggctttg tcgatcattg cgcaagtctg
    42601 ggcggtcata tgctggctgg cctggtaata aagcaccgtc gccagcgccg catacagttc
    42661 agcgttctca ccacgtagtt gcaacgcctg acgatacgcc agcagcgaat tgctgtaatc
    42721 gttttgccac agatagtact cgcccagtaa cgcccattgt tcgctgtttt gtggattagc
    42781 gcggatttta tcctgcaatg cctgaagctg cgcttccggg gtttgctggc tggcaaactg
    42841 atgtagcgga tcgcgctgac gctgatactc cgcacgtacc gcctgccatt ttgggcttaa
    42901 cagataactg ccgacacaga ggaataccat caatatcgtg agtgttgtca gcattttcac
    42961 cggtatttga ggctgtttca tcgcttagcc ctcactcgcc agaggatcag tgccatcagc
    43021 aataacaaca ccactggcag cgcccacaac accagcgtct gacccgttaa cggtgggtta
    43081 tagcgaacaa aatctccgta gcgttcggtc atccagccaa tgatttcgac ttcgttcttc
    43141 ccctccgaca ccatgctata aacctgatgg cgcatactga ccgccaccgg cgcattggat
    43201 tcgagtaagt tttgattctg gcactgcgga caacgtaact ggctggcaat atttaacgcc
    43261 tgttgctgtt gttgcggatt ggcgaattgc caggtgtcta cgacctgagc gcgcgcaaaa
    43321 caggtaaata acagcagtag cgtgagaagc cctttattca tcgcgcttct tcccccgcca
    43381 tccgcttaac aatgcgcccg caatcatcaa caatcctcca ccccagatcc agcgcacacc
    43441 gctttgtaca tacaaacgaa aagcgtaacg atccgctccg gttttttcac ccatgaccgc
    43501 ataccagtca tggatgccgt tccagcgaat tgacggttcc atcatttgct gacggcgtgc
    43561 ttcataaaaa cgccgctccg gcgttaattc accaatgcgt tgctgatggt caaacagcgc
    43621 cactatcgct ttttcgctgg tgtaattgcc tttggcttgc agatcgaggc gctcaaaacg
    43681 gaaggtgtat cctgccagcg tcacctgctg acccggctgt aaattcaggc tgatctcctg
    43741 acggctgacg ctggaaacca cgatccctgc ggcaaataac agcacgcccg catgagcgac
    43801 cagcgccgga agctgcgcgc gtttgccaga gacaaacgtc gccagcacaa tcaccaccag
    43861 catcaacaga ccaaacggta acgtcgcgcg gttaaaatac ggcgcgccaa cggagaggcg
    43921 tccccagccc agcagaccgt aaatcatcgg ataaagcgtt cccaccagca cgatcagtag
    43981 cactgcgcaa aacagcaaca gcgtagcgag gattaacatt tcccgcgata accccgaaaa
    44041 acgcaccgcc gggccaccgt cccgcgctcg ccagccatac agagccaaag acgcaaggct
    44101 aatcagtgca aacaggctga acaacggcac ggcgcggacg ttatccagtg cgaacgcatg
    44161 aaccgaaacc agaatgccag aacggacaat taaggtgccc agcagcgaca gcatcagagt
    44221 aactatcgcc agcaacagcg accagtggca aaaaatcccc cgctggcgtg tcagggataa
    44281 actgtgcagc agcgcagtgg cagaaagcca gggtaataaa gaggcatttt ccaccggatc
    44341 ccagaaccac cagccgcccc agccgagttc gcagtaggcc caccaggaac cgaggatgat
    44401 ccccgccgtt aatgcactcc agccaggtaa cgcccagcgc cagcaaattc gggcgctggc
    44461 cgcatcaaac tcgccgcgca gtagactcgc cagcgccacg ctcgccgcta ccatcaaacc
    44521 gccatagcca aggtaaagca gcggtggatg aaagataaga ccgggatgtt gcagcatcgg
    44581 attgagatcg cggccttcga ttgctggtgg aaatatccgc acgaagggat cggaccacag
    44641 caccacaaac agtagcagtg cggcgagcat gagagataaa acggctaacg tcagcggaaa
    44701 tagcggatcg gtttgctgcc gataatgcca ggcaaacagc gcgctccagg cggaaagcag
    44761 cagcacccaa agcagcagcg aaccttcatg gccgccccac accgctgcca gcttgaattc
    44821 ccacgacagc aggctatagc tatgttgcgc gacataaatg accgagaaat cgctgatgag
    44881 aaaacaatac gtcagtacgc caaaggcgag cagcaggagc gcgaactgcg ccagaatgcc
    44941 gatgcaagtg agtcgcatca tggcaggcca gcgcaaccgc actcccgcaa aggccgtcaa
    45001 cggggtcaac acgttgaccc cgagacttaa caacaacgcc agaaaaccga cttcaggaag
    45061 gaatacgtcc agaaaccacc tcttttctgg cttacgccac cgtcaactgt ccggcgtaga
    45121 gaatgaaaaa gcgcagcatc aacacgccga ccagactggc cccacacgcc gccagcacgg
    45181 caggaatgcc ggaactgcga ttgacccacg gtttgagcaa cattggcaca atcagcccca
    45241 gcccggcgac accaagccag aaccaccacg tccagaaacc gccacctaat gccgccacca
    45301 gcgcacgaac tttaccgtca tcgcccagcg ccagaccgac aaaaaacgcc accagcagga
    45361 agatttcacc ccataccacg gggatttcca tccggtgaac aaagtgcgct tccgtggaat
    45421 gcgggttact gcgttggcgt atcgccatgg cgatcagcgc caccgccgca ccggacgaga
    45481 tgccggagaa gaggaacagc accggcagga tcgggttatt gaggaacgga tacgatttca
    45541 gcgccgacag cagaaagccg gtataagccc ccagcagcac cgccaacacc agcatcagcg
    45601 tttccagtcc gcgatgaacg ggcgtcagca ggctcagaac cttttgcacg atccccagct
    45661 tcggcaacca gcgttgttgc agggcaagca aatcatgttc aaagattttc gccagccaca
    45721 gcaccagcac caccatgtag agctgaaaca gcatcacccc catcgacatc accgaggtaa
    45781 aactgtagtg gaacatcagc ttccagaagg tccacggtct tgtcaggtgg aagacgagga
    45841 tcaacagacc gaggatcacc gcgcccggcc cgacaatcag cgtggtgcgc agcaacgtac
    45901 tgtctgcacc gcccgcctgc gggtagaagc gacgtaacag cacggccagc gtcaccagac
    45961 cggcagaaat accaatcaaa aacaggtaga tggcaatcgg ccagtcccac accagcgatt
    46021 caaaatgaaa tgcggaagtc tgcgtcattg gctcacctcc ccgtatttaa acggtacgcg
    46081 gtacagcttc ggtttggttc ccagcgccag cttgtagcgg taagtaggct tctggcgcag
    46141 cagttgcgaa atctcactgt taggatcgtc cagattgcca aacgtcagcg ccttcgtcgg
    46201 gcaagcctca acacacgcgg gcagcttacc ggcctgcaaa ttggttttac ggcagaaatc
    46261 gcatttgtcc gccgtcttcg tgaccggatg gataaagcgc acgcggtacg gacaggcggc
    46321 gatgcagtac tgacaaccga cgcagagatc aggattaaca tcgacaatcc cgctagcggc
    46381 atcacgaaaa gacgcaccgg tcgggcagac gtcaacgcac ggcgcatgat cgcagtgctg
    46441 gcaagactta cggaagaagc gatatttcac atcaggaaat tcgccctgcg gctcgctacg
    46501 aataatcgtc aggcgcgaga cgccttccgg caccttgttc acttcccgac aagcatccat
    46561 acaggcggta cagccgatgc ataacgactc gtcatgcacc atgccgtaac gcaccccatt
    46621 gatattcaac gttttcgcca ccacgcgccc tgcggtgccg ctgacggctg ccagcacgcc
    46681 gacgccggtg agaaactggc gacgagacca ggtcatggct gctccttaag caacggaaca
    46741 gacgccgggt taaagttcgg attggtgcgc tgatcgctgt ggcaatcgac gcaaatctta
    46801 atccgtcctt tgtcacttaa cgtctgcatc gtatcttgct gtggatgcag ggaatggcag
    46861 ctggcgcacg ccactttggt tacgtggaca tcgtgcggcc agaacgcttt ttgcaactgt
    46921 tccggcagat gacaggacat acagacgctg ttctgctctc caaccttgta catcggctcg
    46981 ttaaagcgca tcacatcttt caccccttcg cggtgttgcg gtgatggctg gccgtgacag
    47041 ttggtgcagg tgaccggcag tttgttattc gggttgataa cggaagcatg tttgccatgc
    47101 ataccttcgg tatctggttt atgacagtcc agacaggcgg catccgggtt acgctgctgg
    47161 gtaacttcgt agcgatcatc agatgcctgc gccgcagggg tagcggtaat tccgttcagg
    47221 ctccacaaca ggcccgacgc cagcaccccg gcagttaata acgaacgtaa tacgctcata
    47281 ttcactccat tgtgttttcc gccccttgcg aggcggaacg gggttattgg cttaacagac
    47341 cgtttttacg tgcctgttct tcccactgcg ggatcaccgt tttgatgaag tcctgcttct
    47401 cggccttgat ttgttccatg ttcaggccaa tggcctgctg ggctttctct ttggttgaga
    47461 tatccgggat ctggatttca tgggtgatgc ctttggtcgc cagcaggcgc gccagtttgg
    47521 tgcgtgcatc cgccgcttta tccatcgccg taccgagcat ccgtaaacct tcttccggtg
    47581 cgtgcatatg aatgccgtgg gaagcgatcg ccagatccca gcgccactgg gcatgacgga
    47641 tatcgtcctg aattggcttc atttcagctt ccgtcgcgcc tgcatccagc gctgctttcg
    47701 cttcgaagtg agcgtgaacc agttgatctt caaccttgat tttcaggtcg ttaatcgact
    47761 gcttacgttc cgcgaccact ttttgcaagg cagctttgtc ctgggtatgg cagttcgcac
    47821 aagtctgggc gaagttatca aacggattac caattttatg gtcggtatag agtttgcctt
    47881 cggcgttctg cacttttggc atatggcagt cgatacaggt cacgttgttt ttaccgtgaa
    47941 tgcccgctgt ccaggtttca tattccgggt gctgcgcttt cagcattggc gttttcgaca
    48001 gggagttagt ccagtcagag aaggcaattt tgtcgtaata ctgctccata ttttcgactt
    48061 tcatgccgtc atcccacggg aatttaaccg ctttgttttt gccgtcgaag taatactcca
    48121 catggcactg accgcaaacc atcgattgct ggtcgaaacg tccggctttc tcaaaaggtt
    48181 taccaatggc ttccatcgcg cgagccgcat acggacggga aagggttaac tccggtttgc
    48241 ctttggcgaa ctctggtgag gcggtgttat ggcagtcggc acaacctaag ttgttgacga
    48301 tttccgggcc gccgcgcgcc cacttaccgt ggaagtagcc atcttcgccg tctttctgga
    48361 tcagacgcgc cacatccggg cttttacaac tccagcacgc catcggtagc ggaccatctt
    48421 cagcgttttt cggcgcaccg gtacgcaggg tttcacgcac atcggtcaca gcaaaggcat
    48481 gtccacgcgg cttgttgtaa tcgcgcgaga agggataccc cgcccacagg atcaccagcc
    48541 gtggatcttc cgccagggcg tcaacacgct ctgactgttc cgaggtggct ttccaggaga
    48601 gatattgatc gggatgctgc ggggcaaagg tttcattctt cgcttccaca gttacaggtt
    48661 ttgcgggagc tgccgtttgt tcagcgtgaa cagaagtgaa aaagaaaaaa ggaatcaata
    48721 agctgaagat acggcgtgcg tttattttta tccttgtcat aggggcttca tccgaattgc
    48781 gccattgtta caatggcggt ttttattgtt tttcacgaca gtaaccgcac ctacactgtc
    48841 atgacattgc tcgcccctat gtgtaacaaa taaccacact gtgaatgttg tctttaatca
    48901 attgtaagtg catgtaaaat accactttag agttagttag tatcttcctc tttttcaaca
    48961 gcaatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttgctg accttcaaaa
    49021 attaccctgc cgtttatttg cacaattcta cttttgcgtg atctgtcgcc caaatactaa
    49081 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa caccctacaa
    49141 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    49201 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    49261 taccttctgg ggcgaacagg gaaaaattct tgactggatc aaaccttacc agaaggtgaa
    49321 aaacacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    49381 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgta ccgccatcat
    49441 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    49501 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    49561 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    49621 cgcggtgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    49681 tgattccaac tcacgactgg tgatcacttc cgacgaaggt gtgcgtgccg ggcgcagtat
    49741 tccgctgaag aaaaacgttg atgacgcgct gaaaaacccg aacgtcacca gcgtagagca
    49801 tgtggtggta ctgaagcgta ctggcgggaa aattgactgg caggaagggc gcgacctgtg
    49861 gtggcacgac ctggttgagc aagcgagcga tcagcaccag gcggaaaaga tgaacgccga
    49921 agatccgctg tttattctct acacctccgg ttctaccggt aagccaaaag gtgtgctgca
    49981 tactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    50041 tccgggtgat atctactggt gcaccgccga tgtgggctgg gtgaccggac acagttactt
    50101 gctgtacggc ccgctgacct gcggtgcgac cacgctgatg tttgaaggcg tacccaactg
    50161 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    50221 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgatcg agggtaccga
    50281 ccgttcgtcg ctgcgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
    50341 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    50401 cgaaactggc ggtttcatga tcacgccgct gcctggcgct accgagctga aagccggttc
    50461 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    50521 ggaaggcgct accgaaggca gcctggtgat caccgactcc tggccgggtc aggcgcgtac
    50581 gctgtttggc gatcacgaac gttttgaaca gacctacttc tccaccttca aaaatatgta
    50641 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    50701 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagattg agtcggcgct
    50761 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    50821 tcaggcgatc tacgcctacg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    50881 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    50941 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    51001 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
    51061 cgtagtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    51121 tgccggatgc gagtcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    51181 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    51241 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
    51301 gagaatctgt gatgaatggc actatttatc agcggataga agacaatgcg catttcaggg
    51361 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    51421 atatcggctt tattttactg atcgccttcg cgcccggctg gctggcacgc cgctgaatcc
    51481 gaacaccagc gtcacacgcg gtattccaat tggtgttgga gtgattgtga tctcctttgt
    51541 tctcaccggt atctacatct ggcgggcgaa cggcgaattc gaccgtctta ataatgaagt
    51601 cctgcatgag gtacaagcat catgaaaaga gttctgacgg cgcttgccgc cacactccct
    51661 ttcgcagcta acgccgcgga tgctattagc ggggccgtag agcgccagcc aacgaactgg
    51721 caggcgatta ttatgttcct gattttcgtc gtgtttacgc tcggcattac ctactgggca
    51781 tcaaaactcg tacgttctcg tagcgactac tacaccgcag gcggcaatat cactggcttc
    51841 cagaacgggc tggcgattgc cggggactat atgtccgccg cctcattctt ggggatctcc
    51901 gcgctggtgt ttacctccgg ctatgacggc ttaatttact cgctgggctt cctggtgggc
    51961 tggccgatca ttttgttcct gattgccgaa cgtctgcgta acctggggcg ctacaccttt
    52021 gccgatgtgg cctcttaccg tctgaaacaa gggccgattc gtattctttc ggcctgtggt
    52081 tctctggtgg tggtggcgct ttaccttatc gcccagatgg tgggcgcagg taaactgatc
    52141 gagctgctgt ttggccttaa ctatcacatt gcggtggtgc tggtcggcgt gctgatgatg
    52201 atgtacgtcc tgttcggcgg catgctggcg accacttggg tacagattat caaagccgtg
    52261 ctgttgctgt tcggtgccag ctttatggcc tttatggtga tgaaacacgt cggctttagc
    52321 ttcaacaatc tgttcagtga agcgatggcg gtacacccga aaggtgtcga catcatgaag
    52381 ccgggcgggc tggtgaaaga tccgatctcc gcactctctc tgggtctggg actgatgttt
    52441 ggtacggcgg gcttgccgca cattctgatg cgcttcttta cagtcagcga tgcccgcgaa
    52501 gcacgtaaga gcgtgttcta cgccaccggg tttatgggct acttctatat tctgaccttt
    52561 attatcggct tcggcgcgat catgctggtt ggtgcgaatc cggaatataa agacgcggcg
    52621 ggccatctga ttggtggtaa caacatggcg gccgttcacc tggcgaatgc agtgggcggc
    52681 aacctgttcc tcggttttat ttcagcggtt gctttcgcca ctatcctcgc ggtggttgcg
    52741 ggtctgacgc tggcgggcgc atccgcggtt tcgcatgact tgtacgctaa cgtcttcaaa
    52801 aaaggcgcga ccgaacgtga agagctgcgg gtatcaaaaa tcaccgtact gatcctcggc
    52861 gtgattgcga ttatcctcgg cgtgctgttt gagaatcaga acatcgcctt tatggtgggg
    52921 ctggcgtttg ccatcgcggc gagctgtaac ttcccgatca ttctgctttc tatgtactgg
    52981 tcgaaactga ccacgcgtgg cgcgatgatg ggtggctggc tggggctgat taccgcagta
    53041 gtactgatga tcctcggccc gacgatttgg gtacagatcc ttggtcacga aaaagccatc
    53101 ttcccgtatg aatacccggc gctgttctct atcaccgtgg cattcctcgg catctggttc
    53161 ttctcggcaa ccgataactc agcggaaggg gcgcgcgagc gtgaactgtt ccgcgcgcag
    53221 tttatccgct cccagaccgg ctttggcgtt gagcaaggcc gcgcacatta atctctcccc
    53281 ttccccggtc gcctgaccgg ggaatactct tcctctccag catgcatcac cttttcccaa
    53341 aatattaaac aaataaactc attaaaaaat gagcgatttt tgacagtcgt agaaaatgat
    53401 a
//
LOCUS       DAZPLI010000011        51221 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZPLI010000011 DAZPLI010000000
VERSION     DAZPLI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51221
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.011"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..1334)
                     /locus_tag="ACHUU3_000603"
     CDS             complement(<1..1334)
                     /locus_tag="ACHUU3_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR2080770.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
     gene            complement(1380..2138)
                     /locus_tag="ACHUU3_000604"
     CDS             complement(1380..2138)
                     /locus_tag="ACHUU3_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2080771.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(2170..2883)
                     /locus_tag="ACHUU3_000605"
     CDS             complement(2170..2883)
                     /locus_tag="ACHUU3_000605"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR2080772.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            3057..3749
                     /locus_tag="ACHUU3_000606"
     CDS             3057..3749
                     /locus_tag="ACHUU3_000606"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR2080773.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPRPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(3798..5297)
                     /gene="pitB"
                     /locus_tag="ACHUU3_000607"
     CDS             complement(3798..5297)
                     /gene="pitB"
                     /locus_tag="ACHUU3_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR2080774.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(5585..7444)
                     /gene="gss"
                     /locus_tag="ACHUU3_000608"
     CDS             complement(5585..7444)
                     /gene="gss"
                     /locus_tag="ACHUU3_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR2080775.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            7649..8515
                     /gene="yghU"
                     /locus_tag="ACHUU3_000609"
     CDS             7649..8515
                     /gene="yghU"
                     /locus_tag="ACHUU3_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR2080776.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(8544..8625)
                     /locus_tag="ACHUU3_000610"
                     /pseudo
     CDS             complement(8544..8625)
                     /locus_tag="ACHUU3_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8638..8886)
                     /gene="hybG"
                     /locus_tag="ACHUU3_000611"
     CDS             complement(8638..8886)
                     /gene="hybG"
                     /locus_tag="ACHUU3_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR2080777.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(8899..9240)
                     /gene="hypA"
                     /locus_tag="ACHUU3_000612"
     CDS             complement(8899..9240)
                     /gene="hypA"
                     /locus_tag="ACHUU3_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR2080778.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(9233..9721)
                     /gene="hybE"
                     /locus_tag="ACHUU3_000613"
     CDS             complement(9233..9721)
                     /gene="hybE"
                     /locus_tag="ACHUU3_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR2080779.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNSDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(9714..10208)
                     /locus_tag="ACHUU3_000614"
     CDS             complement(9714..10208)
                     /locus_tag="ACHUU3_000614"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR2080780.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(10208..11911)
                     /gene="hybC"
                     /locus_tag="ACHUU3_000615"
     CDS             complement(10208..11911)
                     /gene="hybC"
                     /locus_tag="ACHUU3_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR2080781.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYGGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(11908..13086)
                     /gene="hybB"
                     /locus_tag="ACHUU3_000616"
     CDS             complement(11908..13086)
                     /gene="hybB"
                     /locus_tag="ACHUU3_000616"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR2080782.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(13076..14062)
                     /gene="hybA"
                     /locus_tag="ACHUU3_000617"
     CDS             complement(13076..14062)
                     /gene="hybA"
                     /locus_tag="ACHUU3_000617"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR2080783.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(14065..15183)
                     /gene="hybO"
                     /locus_tag="ACHUU3_000618"
     CDS             complement(14065..15183)
                     /gene="hybO"
                     /locus_tag="ACHUU3_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR2080784.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(15372..15659)
                     /gene="yghW"
                     /locus_tag="ACHUU3_000619"
     CDS             complement(15372..15659)
                     /gene="yghW"
                     /locus_tag="ACHUU3_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR2080785.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(15778..16665)
                     /gene="yghX"
                     /locus_tag="ACHUU3_000620"
     CDS             complement(15778..16665)
                     /gene="yghX"
                     /locus_tag="ACHUU3_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR2080786.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            16822..17862
                     /gene="gpr"
                     /locus_tag="ACHUU3_000621"
     CDS             16822..17862
                     /gene="gpr"
                     /locus_tag="ACHUU3_000621"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR2080787.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(17902..18396)
                     /locus_tag="ACHUU3_000622"
     CDS             complement(17902..18396)
                     /locus_tag="ACHUU3_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR2080788.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            18587..19471
                     /gene="yghA"
                     /locus_tag="ACHUU3_000623"
     CDS             18587..19471
                     /gene="yghA"
                     /locus_tag="ACHUU3_000623"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR2080789.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(19631..20056)
                     /gene="exbD"
                     /locus_tag="ACHUU3_000624"
     CDS             complement(19631..20056)
                     /gene="exbD"
                     /locus_tag="ACHUU3_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR2080790.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(20063..20797)
                     /gene="exbB"
                     /locus_tag="ACHUU3_000625"
     CDS             complement(20063..20797)
                     /gene="exbB"
                     /locus_tag="ACHUU3_000625"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR2080791.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            21049..22236
                     /gene="metC"
                     /locus_tag="ACHUU3_000626"
     CDS             21049..22236
                     /gene="metC"
                     /locus_tag="ACHUU3_000626"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR2080792.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            22376..23035
                     /gene="yghB"
                     /locus_tag="ACHUU3_000627"
     CDS             22376..23035
                     /gene="yghB"
                     /locus_tag="ACHUU3_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR2080793.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(23075..23974)
                     /gene="yqhC"
                     /locus_tag="ACHUU3_000628"
     CDS             complement(23075..23974)
                     /gene="yqhC"
                     /locus_tag="ACHUU3_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR2080794.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESALQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            24168..25331
                     /gene="yqhD"
                     /locus_tag="ACHUU3_000629"
     CDS             24168..25331
                     /gene="yqhD"
                     /locus_tag="ACHUU3_000629"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR2080795.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            25436..26263
                     /gene="dkgA"
                     /locus_tag="ACHUU3_000630"
     CDS             25436..26263
                     /gene="dkgA"
                     /locus_tag="ACHUU3_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR2080796.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            26463..27389
                     /locus_tag="ACHUU3_000631"
     CDS             26463..27389
                     /locus_tag="ACHUU3_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR2080797.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            27440..27697
                     /gene="yqhH"
                     /locus_tag="ACHUU3_000632"
     CDS             27440..27697
                     /gene="yqhH"
                     /locus_tag="ACHUU3_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR2080798.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(27740..29959)
                     /locus_tag="ACHUU3_000633"
     CDS             complement(27740..29959)
                     /locus_tag="ACHUU3_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR2080799.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(30070..31482)
                     /gene="ftsP"
                     /locus_tag="ACHUU3_000634"
     CDS             complement(30070..31482)
                     /gene="ftsP"
                     /locus_tag="ACHUU3_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR2080800.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(31557..32294)
                     /gene="plsC"
                     /locus_tag="ACHUU3_000635"
                     /gene_synonym="parF"
     CDS             complement(31557..32294)
                     /gene="plsC"
                     /locus_tag="ACHUU3_000635"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR2080801.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(32527..34785)
                     /gene="parC"
                     /locus_tag="ACHUU3_000636"
     CDS             complement(32527..34785)
                     /gene="parC"
                     /locus_tag="ACHUU3_000636"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR2080802.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(35331..35813)
                     /locus_tag="ACHUU3_000637"
     CDS             complement(35331..35813)
                     /locus_tag="ACHUU3_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR2080803.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(35866..36258)
                     /gene="ygiW"
                     /locus_tag="ACHUU3_000638"
     CDS             complement(35866..36258)
                     /gene="ygiW"
                     /locus_tag="ACHUU3_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR2080804.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            36410..37069
                     /gene="qseB"
                     /locus_tag="ACHUU3_000639"
     CDS             36410..37069
                     /gene="qseB"
                     /locus_tag="ACHUU3_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR2080805.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            37066..38415
                     /gene="qseC"
                     /locus_tag="ACHUU3_000640"
     CDS             37066..38415
                     /gene="qseC"
                     /locus_tag="ACHUU3_000640"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR2080806.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            38525..39106
                     /gene="mdaB"
                     /locus_tag="ACHUU3_000641"
     CDS             38525..39106
                     /gene="mdaB"
                     /locus_tag="ACHUU3_000641"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR2080807.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            39137..39451
                     /locus_tag="ACHUU3_000642"
     CDS             39137..39451
                     /locus_tag="ACHUU3_000642"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR2080808.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(39499..41391)
                     /gene="parE"
                     /locus_tag="ACHUU3_000643"
     CDS             complement(39499..41391)
                     /gene="parE"
                     /locus_tag="ACHUU3_000643"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR2080809.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(41420..42001)
                     /gene="yqiA"
                     /locus_tag="ACHUU3_000644"
     CDS             complement(41420..42001)
                     /gene="yqiA"
                     /locus_tag="ACHUU3_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR2080810.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(42001..42828)
                     /gene="cpdA"
                     /locus_tag="ACHUU3_000645"
     CDS             complement(42001..42828)
                     /gene="cpdA"
                     /locus_tag="ACHUU3_000645"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR2080811.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(42853..43275)
                     /locus_tag="ACHUU3_000646"
     CDS             complement(42853..43275)
                     /locus_tag="ACHUU3_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR2080812.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(43276..43905)
                     /gene="nudF"
                     /locus_tag="ACHUU3_000647"
     CDS             complement(43276..43905)
                     /gene="nudF"
                     /locus_tag="ACHUU3_000647"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR2080813.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            44110..45591
                     /gene="tolC"
                     /locus_tag="ACHUU3_000648"
     CDS             44110..45591
                     /gene="tolC"
                     /locus_tag="ACHUU3_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR2080814.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            45739..46410
                     /locus_tag="ACHUU3_000649"
     CDS             45739..46410
                     /locus_tag="ACHUU3_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR2080815.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            46416..47576
                     /locus_tag="ACHUU3_000650"
     CDS             46416..47576
                     /locus_tag="ACHUU3_000650"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR2080816.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(47614..48402)
                     /gene="ygiD"
                     /locus_tag="ACHUU3_000651"
     CDS             complement(47614..48402)
                     /gene="ygiD"
                     /locus_tag="ACHUU3_000651"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR2080817.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            48545..49318
                     /gene="zupT"
                     /locus_tag="ACHUU3_000652"
     CDS             48545..49318
                     /gene="zupT"
                     /locus_tag="ACHUU3_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR2080818.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(49376..49546)
                     /gene="yqiD"
                     /locus_tag="ACHUU3_000653"
     CDS             complement(49376..49546)
                     /gene="yqiD"
                     /locus_tag="ACHUU3_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR2080819.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(49807..50460)
                     /gene="ribB"
                     /locus_tag="ACHUU3_000654"
     CDS             complement(49807..50460)
                     /gene="ribB"
                     /locus_tag="ACHUU3_000654"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR2080820.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(50564..50712)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            50834..51124
                     /gene="ubiK"
                     /locus_tag="ACHUU3_000655"
     CDS             50834..51124
                     /gene="ubiK"
                     /locus_tag="ACHUU3_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR2080821.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT        12767 a        13693 c        12788 g        11973 t
ORIGIN      
        1 gctgcgttat aaagcaagga atgacgctga aaaaatgcct ttaaagttgg aattaacgag
       61 agcaccacat ccagacattg cccaccgaag taagcaaacg ctgctcccag cacaccaaac
      121 agatgacaga acatgaacag caatacgatg tagccgattg aggcgatggt ttgcgccacg
      181 agaaaagcgc gctgttttcc cgccatcaat agcaatgact cctgcgggaa acccagcatc
      241 gaaatcacaa ttgcgcccag catcacctga atcaggtcat acgcttcgag atacttaacg
      301 ccaaacacca gcgaaatgag cggcttgccg gcgatcaaca ccgcgagcgc caccagaata
      361 ccgattccgc ccgccagtaa accggacttc acacctaaca accacggtct ggtggtgcgc
      421 ggatctaaac gcatcacctc tgggtaaaaa cttttaccca gcaaacccgc tggcgttccg
      481 gcggcgtcga agaatgtcat ggcgatttta aataacccgg cggcagcggg tcctaacacg
      541 atccccacca gcactgtgct gcacgagtta cgcgccgacc agatggagtg ggcaatgttg
      601 gttgaccaga caaaactcca cgcgcctttg atgtgtcggg cagactcaaa cagattcaat
      661 ttgaaggcat tatggatatt tctgcggcgt aattcgcgcg ctgcaaacca ccagtacatg
      721 gtgccgccaa ccagattcga cacgtaccag gcaataacaa aacccgcaaa accaaagtca
      781 aaataccagg ctacgacgct ccccgctgcg cgcagaaaag gtttcgtcgc ctgctgtaca
      841 gcaattaaat cgaagcgatc taccgcacgc agaatgccgg tcggcgtgga ggaagccatt
      901 gaaggaatga gcgtgcaata cagcgccgcc agccaaaagc tttggtcatc cagacctaat
      961 gaatgggaaa ggaatggcag taaggcaatg ccgccgacaa tcgccaccgc gccgctgacg
     1021 atatccagcg agaaggaaaa tgagacgaca ttgcgaaatt gctgcggatt attgttggtc
     1081 aaggctggcg ttccgtactg aaccaccagc tgccatgtct gaaacttaat aaaatcgctg
     1141 atcgacttgg cgtacgattg cacaatcacc agtacgccaa acatggcggg cgtcatccct
     1201 ttaccggcac acgagagcgc caacagcccc agcaaggcgc tcacgacatt actggagcct
     1261 aaccaggcgc tattgcgaat aatggtgcga aacgcgccat ctgcaaacca atgtttgatg
     1321 ttaaaacccg ccaattcagc ctgaccttat cgttgatagt aaaaagtatc ccgccagcct
     1381 taagttaaac ttcggcggtc agaaacgatg gcaaccagag aaaccgcctt ctgtgcctgt
     1441 tccagcactt cgctgtaggg cgctctggaa tcaatctcaa gaatttttgt gccgttatag
     1501 ccaatcttcg acatgacacc gattttgtcc tgcagctcgg catagtcatg gtcaggcttg
     1561 cgggagatgg cagtctcaat atcaatgccc aggcgaataa ttaattccgg gcgatattgc
     1621 gccatttgtt ggtataaacg ccgttcgcgc tgcgccagaa acatgctgat tttcccggtc
     1681 gcacgttcga cgccaatccc cggtccgtcg taataaaagc ccgaaatttc agcctgcggg
     1741 aagcgatcgc tgaccaccag aacgccactt tgcgccagtc gctgaacctt gcgtagattc
     1801 gccattcttc gcagcgagaa gcagtacata atcaccgccg cccatagcgc cggagatttg
     1861 gttttcatgc tttgggtttt cgaggatttg gccgccagtc gccgttccag ccagacgcca
     1921 accaacggta atcgtttgat tttgtcgccg tcttcgcccg agagcagccc cagatagcgc
     1981 cgctcggttt gccagtgctg ttgcaatgat ttcaccagat cggtggtcag cgtggattta
     2041 ccggtgccat cgcacccgac caccgcaatt agccccggaa tgtagctggg ctgcggcgcg
     2101 gttgtactat taacagtttg tgtttgtagt gcatccattc gttaaatcat cccctatcca
     2161 ttttggtgat caggaaagcg cggcgtgaat tgcacgtagt gacgcttgca ggatttcatc
     2221 ggctggttgc cgaccatcca gttcaaggat cctggcgccg ttgaaagtca gttgcggcgt
     2281 aacggcgatt ttttcctgta acgctgccag ttggtggtca ggtttacgcg caaacgcggt
     2341 ttgttcatca atgccaagac gaatcaacaa tacgggcaaa taagatgcca tccattggta
     2401 cagcttcagc tcgcgctgcc ttaacatttt tatccaaccg ttaccgcccg tggtttttgc
     2461 caattgcggg ccatcaaagc gaaaacccgg cacttcaacc tgcgggtagc ggtcggtgat
     2521 gagcagaaag ccttgctggc ttttacacaa cattttgcga aacttgtacg cccgccagca
     2581 ggaaagcaga tagataacca gagcagtaat attgccaggc ggtgttgagg gtttttcgtg
     2641 cacatgtgcc gctttacttc gcagataacg cccaaaaggt gcgccaataa cagggagctg
     2701 tgaaatccat tcgccaatta gcccggacga ttgcccgaga taaatgtgtt ctgttggcat
     2761 tcttgctgcc agttcattta ccaggcttgc cgtgagggtc gatttacctg aaccatcaca
     2821 tcctacaatg gcaataacac gcactggcgt ggaattaatt attgacatat tacgttgatt
     2881 cacgaaaaac ccggcagtaa ataatgtatt gaatattacg tggtccgtgc tcagaatatc
     2941 cgttcaacct tattgtgaca atggttaatt gtgacattga cctgaattac gctttcataa
     3001 aaacatatta accaaataaa tatttttaat ggatatttaa attaaaggat atattcatgc
     3061 agtcaataac acctccatta attgccgtta ttggtagcga tggttcaggc aagtcaacgg
     3121 tgtgtgaaca tcttattacc gttgtcgaaa aatatggtgc tgccgaaaga gttcatttag
     3181 gaaaacaggc cggaaatgtc ggtcgtgcag tgacaaaatt accgttgatg ggaaaatcct
     3241 tacataaaac aattgaacga aatcaggtga aaacagcaaa aaaattgcct agaccagttc
     3301 cggcgctggt aattacagcg tttgtcgccc gtcgcttact gcgctttcgt catatgcttg
     3361 cctgtcgtcg tcgcgggtta attgttctaa ccgaccgtta tcctcaggac caaattcctg
     3421 gcgcttacga tggtacggtg ttcccaccta acgttgaagg tggtcgtttt gtctcatggc
     3481 tggcaagcca ggaacgtaaa gcgtttcact ggatggcgag ccataagcct gatctggtca
     3541 tcaaactcaa tgttgacctt gaagttgcct gtgcacgtaa acccgaccat aaacgggaat
     3601 cgctggcgag gaagattgcc ataacgccac agttaacctt tggtggtgca caactggttg
     3661 atatcgatgc caatcagcca ctggaacagg tgttggttga tgcagaaaaa gcgattacgg
     3721 attttatgac cgcgcgtggt tatcactagt caaaaatgga aatgcccgat cgccaggacc
     3781 gggcattttc aggaaggtta aatcaactgc aatgctatcc agtacagccc accagaaaga
     3841 aaaattgccg ccggtaaagt aaacacccac gccatcagga tgctggttac cgttttacgc
     3901 tgtaacccac cgccgtccac caccatcgtc cctgctaccg cagacgagag gacgtgtgtt
     3961 gtggagacag gcatcccaat ataactggca aggccgatag acactgccgc cgtcatttgt
     4021 gccgccatgc cttgcgcata cgtcatgccg cgcttaccaa tcttctcacc gatggtcatc
     4081 gctacacgac gccagccaat catggtgcca atgccgagcg ccagtgctac cgccatgatg
     4141 atccacacag gagcgtactc aatggtgctt aacatatcgc tgcgcagttt tttcagcagg
     4201 ttctggtctt ctttactgac gcctggcagt ttcgccagct tcgcggaggt atcagagatg
     4261 cacagcataa tgcggcgcag ctggctgcgc tgactcacgc ttaacggctc gtaactttcc
     4321 atattgcctg gcagcatcgt tttaacgcgc gcaatcgcat caaaggtatt tgccggatga
     4381 cagtgaaact ctgttacttg cgtgccatca gtcgatgctg caggcaatgg aggttccatc
     4441 gcaatcaact tctgcggcag ttcaggatgc tgttgcaggt agtgttcgaa gttggtgacg
     4501 gcatcgcggg tacgggtaat ttcatagccg gacgcgttca tattgacgac gaagccagca
     4561 ggggcaatcc ccaccagtac cagcatcacc aggccgatcc ctttttgtcc gtcgttcgcg
     4621 ccgtgcgaaa acgccacgcc cgcagcggaa acaatcagcg caatacgcgt ccagaatggc
     4681 ggtttacgtt tgcctttttt ctttttgcga tcttccggaa tgcggtgaat acggtcacgc
     4741 tttttcgtcc cgctccagta gcgtcgcagc aggaatatca ggcctcccgc aatgaccagg
     4801 ccgacgatag gggaaacaat cagcgaggag aaaattttgg tcacttcacg caggtttaac
     4861 gcatccatca ccgatgatcc ggttaacagc gcgttggtta aaccgatgcc gataatcgca
     4921 ccaatcaagg tgtgcgaact ggaggccggt aaaccgaaga accacgttcc caggttccag
     4981 ataatcgccg ccagcagcat ggaaaagacc atcgccaggc cgtgggttga ccccatattc
     5041 agcaacaaat cggttggcaa catatggaca atggcatagg caacgctaag tccgcccaat
     5101 aacacgccaa aaaagttaaa aaatgccgcc atcaccacag caagttgtgg ttgcatggca
     5161 cgagtataaa taacggttgc caccgcattc gccgtgtcat gaaaaccatt gattgcttcg
     5221 tagaacaaca caaatgccag agcaagcaat aacaaaagcc ctgtgtatat atcaaggcca
     5281 acaaataaat ttagcatata ttagattacg ccattttgaa tttacgaacg gacgcattat
     5341 cagtgacttt aacggcatgg gcaaagtgaa atatcatttt ttaacgataa aaagagtgtt
     5401 atttcttgtg tatcagacaa taagcattca ttcaaaacct ttagaaataa tcaaaacaca
     5461 ggtattccac tggttaatat attcacatat gaaataaata ttagaacata taaatattga
     5521 atattttgat taatccccgt actgattatt cttcataatc agtacggggt tgctacaaca
     5581 tgtattactt tttcaccaca attaacggtt caatatcact ctcttttttg atgaccagtg
     5641 attcatcacc gcgcaaacac gtcccaccgt agttgccgcc cacggtgaag gtacatacct
     5701 gaatgtattt accgtccact ttcggcaaac accacagttg ctgatagatg tttttctgct
     5761 cggcaaattt accgctggtt ttgtccagca cctcttcatg atggctgacc aggtcgatat
     5821 tgctaccaca gcgaccggca atcggtttca ctgcgtaacc tgttttcacc aattcatcat
     5881 taacagtgaa atcggtatcc agcaggtaac gatggtgcgg gaacagcgac cagaggatcg
     5941 gcagaatcgc tttgttgccg gggatcaccg tccacagcgg ctcaaagact agcacttccg
     6001 ggcgcagcaa tacgtcgata agacgcactt cgttttgcgg atgaccggta cggattggca
     6061 ccgcagcaaa ctcacggtcg ctaacttcac gaatctgatc aaacgcggtt tcccacgccc
     6121 aggttttcca cacgcagtta accagtcgcc cttccccatc aatcagttgc ccggcagcat
     6181 cccagcccag ttcatccaat ccacgcaaga tacgcgtttc aaagcccgcc tggtgcagcg
     6241 cctgctccat aaactgcgcg tgatagtttt cctcgatatc tttgtcctgc atgatatgga
     6301 caaacggacg tgcacgactg tgtttccagg caccagccaa ttcgttaatc agcccttccg
     6361 ccggattgaa gccgttgcct ttatagccct gctccgccca acgttcgagg atcaagcccg
     6421 cttcggtatg acaggaggcg gagtcggcgt tgtactcgta aaccttcagg ccacgctcat
     6481 ccatgcagaa atccatacga ccagtgatca tatggtgacg gcgacgctgc caggagagac
     6541 gcaaacgtgg ccagaggatt ttcgggatgt cgaacagcgc cagcaggttg tcatctttca
     6601 gcaccttgtc ggttgcgtga agatacatca ggtgcagctc gttggtggct ttaattagct
     6661 cctgctcggc actctcggta atggtgtagt aatgataagg atcctgattg atcacctgac
     6721 cgttggcctg cacataggcg ttttgcagcg gatctttttc atccagccat ttaccgtcaa
     6781 actggccttt gttttccagg cgcgctccgc tgattttcag cagctcgcct gcaatttccg
     6841 gctgcggtaa gctgtattca gtatcttccg tctggatcat ccagcccaga atggtggtgt
     6901 catcaaaagt gtctttcagg gtatagcagc cgttttcgac caccatctcc agctcacgcg
     6961 tccactgttg cccttgcggc aacggggaat gaatcacgtt ctgttccgca atacggactt
     7021 tgttgccatg caattgggta atgatggcga catggccagt gtctttaaat tcaccgcctt
     7081 tatcccagat aagaagcgca cccgcgaccg gcgcacgcgg cgagccgtta ggaaatgcct
     7141 gcaatggcag gatgttgtca ttaaccactt ctcgcaggaa gcgcagcgag aaaatctccc
     7201 acgccatacc cacgtcagta aagaccacac cgtaattcag aaagagaaaa cggcgagcaa
     7261 attcaacgca ttgccacttg tggcccatat attcgtcgtc gatatagcta cggaatacgg
     7321 cgtcatcttc gtattcctgc ggatcgagag aactgtaatc tgaagagtag attgctaccc
     7381 cacctggggc gtagcccaat aatgtcccga acggggcatc ctggctggtc gttcctttgc
     7441 tcatcacttt acctttaaca atacaaccta agcagttggc gtaattgttg tgctctgatt
     7501 acctgctcag cgacattaac cggacagagg tcagactaat catacacctc atcgcagcgg
     7561 ctggcgcaaa gggttaaaaa attcacattc tgtacagcaa gtgacctgct acactgcttc
     7621 aacactacca ctcagaaggc aactcactat gacagacaat acttatcagc ccgcgaaagt
     7681 ctggacgtgg gataaatccg ctggcggcgc gttcgccaat atcaatcgcc cggtttctgg
     7741 tccgacgcat gaaaaaacgc tgcccgttgg caaacaccca ttgcaacttt attcgctggg
     7801 aacgccgaac ggtcagaaag taacgattat gcttgaggag ctgctggcgc tgggcgttac
     7861 tggtgcagag tacgacgcct ggctgattcg tattggcgat ggcgatcaat tctccagcgg
     7921 ctttgtcgaa gtgaacccaa actcgaagat cccggcgctg cgcgatcata cgcataatcc
     7981 gccgatccgc gtgtttgaat ctggttcgat cctgctttat ctggcggaga aatttggcta
     8041 cttcctgccg caggatttgg caaagcgtac tgaaacgatg aactggctgt tctggttaca
     8101 gggcgcggca ccgttcctcg gcggtggttt tggtcacttt taccattacg cgccgatgaa
     8161 aattgagtac gccatcaacc gctttaccat ggaagccaaa cgtctgctcg acgtgctgga
     8221 taagcaactg gcgcagcata agtttgttgc gggcgatgag tacaccattg cggatatggc
     8281 gatttggccg tggtttggca atgtggtgtt aggcggcgtg tatgatgccg cagagtttct
     8341 tgatgcgggc agttataagc atgtacaacg ctgggcgaaa gaagtaggcg aacgtccggc
     8401 ggtgaaacgt gggcgtattg ttaaccgcac caacggaccg ctgaatgagc agttgcatga
     8461 gcgccatgac gccagtgatt tcgagacgaa tacggaagat aagcgtcagg ggtaagggtt
     8521 ggtgttcgtc gcagcaagcc atccaggccg gataaggcgt tcacgccgca tccggcaatc
     8581 gtgcacaatg cctgatgcga cgctgccgcg tcttatcagg cctacgaaag ctaatcatca
     8641 cgcgctggta atgtcgtaat ccatttggcg cagtgcgtct aatgtggctt tggcttcatc
     8701 ttcgtcgatg atgctcatgg caaatccgac gtgtaccagc acccactggc ccagtagatc
     8761 ggcagggtta ccttcacaaa tcagggcgat attcacgtcg cgcttgatac cacatacttc
     8821 aacctgcgca agctggtgaa tatcttcacc gacagccagc acctggcctg gaacgccaat
     8881 acacattatt aactccggtt attcaacttc aatacttttg acgatcagcg aatcgccggt
     8941 atcgacacgc aaccgctcgc cgtgacagag cggacactgc gcatcgtgct gatgaatctc
     9001 caccacctgg ctgcaatccc agcaccaagc ctgggcgggt ttatagacga tatgtaaatc
     9061 gcacccttgc gccaccgttc cgtggcagac aatttcaaaa ctaaaacgga cggcgctctc
     9121 ctcaacgcag gagagcgcgc caatttccag ccacacggcg gtgacgcgct taacatcgtg
     9181 ctgctccgcc tgccgttgga taatttcaac ggcgctctgg caaagagaca actcatgcat
     9241 tttcgccact cctgcgacca aacagcaggg cgcgacgccc tgcgtgtggt acatccgaat
     9301 tcgtgactgg cagcgaaagg atcattcgtg cgcagtcatc cgtcaggcgt tggccctctt
     9361 caatcgacat gctgtgcgaa agcggcgaca tcagcgaaca ggagagatat tgcgaaacac
     9421 cgtccagttc gccaacggta aaggtcatag tgccatacgg cagttgcaga ccaatttttt
     9481 cactcacttt gcgcagcggc cagagttgat ccgggccggg gaagataact gcactcagca
     9541 tccacggggt gatcacacac cccgtccact gaccttcgaa cagcgtaaaa tcagaaacat
     9601 acaccggcat tgaaggatgc agaaaagaga gatcgtgcat cgaacgccgg gcaatttctt
     9661 caaacgctgc ctgtacttgc gccttcgggg aggtctggaa acctgctatc tcttcagtca
     9721 tgaatcgcct cccgtgggat ggcttccacg cctgattcgc gcagcgcagc cagaacctgc
     9781 tcaagcgcag gttcaatcat tgcttcaacc gtcggcgtta agccgatgtg tggctccagc
     9841 gattccggga tcacgccgac cagggtcagc tttttcggaa actcaccggt gaagcgcagg
     9901 gccgacaata cgtcggccag gccaagctga tgcggagaga ttttgttggt aaacaacgcc
     9961 ggaacttctt catcccgcag gatcatcatc gttcccggcg tgttcttttt cgacacaatg
    10021 gcatccgcaa taatcagatg atcgcgattt gccatgtcgc caagcagctc cattcccgcc
    10081 gtgccgccat cgaggatctc aacataatcc ggcagaatgt atcgttgctc taacgcttcg
    10141 acaatccgca caccgatggc ttcatcggtc agcaaaatat tgccgacccc taagactaaa
    10201 atacgcatta cagaaccttc actgaaacca cttcgttgcc gtcggcatcc actacgtgta
    10261 ccgcacaggc catgcacggg tcgaaggagt gaatggtacg caccacttcc agcggtttat
    10321 tcggatcagc aaccggtgta cccaccagcg actgctcgta aggaccgacg tcatcattga
    10381 agttacgcgg accagagttc caggttgatg gaacaactgc ctggtagtta ctgataatac
    10441 cgtctttaat aaccatccag tgagagagca taccgcgcgg cgcttcgagg aagccaacac
    10501 ctttgaactc acccgttgcc ggaatgttcg gtttcacaaa ggtggtgtga tcgcctttgc
    10561 cgatattggt gatcagtgca ctgtattgat tttgcaggat atcctgcaat tcgcagcagt
    10621 gaacggtacg accaataata cggcccagcg tggagtgcag ctgcgccact tccagcgtgt
    10681 tgccagtcag tttctgataa atcgcaacga tttcattcag tttgttctgg gtagattcgc
    10741 gacctgccgc cagtttcacc agcatattag ccagcggacc cacttctacc gttttgccgt
    10801 agaaagtcgg tgatttcacc caggaatatt tcccgtcgtc agaccaacca tcataagccg
    10861 gaatggtggt accttcccac ggtgcctgcg gcgcttcgtc tttataccag gagtgcttcg
    10921 cgctttcctg aatgcctttg atcaggtatt catcggaatg agaagtgatc ggacgatacg
    10981 aggacagatc cgcattctca atgtagccgc ccgggaacag gaagctgccg tttttactgt
    11041 cggtcgggaa ttccggcacg ctcaggtagt tcaccgcacc tttaccgcgc gtcagccatt
    11101 ccgggtagaa cgcggcaata actgcggtat caaccttata aacctgttca acaaagtcgc
    11161 tcagcttgtc gatgaaagac ttgatgtaca tcaggcgctc aaggttcagc acgcccaaac
    11221 cgtcgaggtt gatcgggttc gcgacaccac ctaccgccag gttctgaatg tgcggcgttt
    11281 taccgcccag cagcgccacg acgcggttag cgtcacgctg gcactccagc gcctgcaggt
    11341 agtgcgctac cgcaatcagg ttcacttccg gcggcagttt catcgccggg tgacccccgt
    11401 agccattggc gaaaataccc aactgaccgc tggcaaccag atctttgatc ttgttctgaa
    11461 ctttagtgaa ctcttccgga ctgttcagat gccaggtcga aacgcctttc agcatttcgg
    11521 aggctttggt tgggtcagct tgcagtgcag aagtgatgtc cacccagtcc agcgccgaaa
    11581 gctgatagaa atgaacgata tggtcatgcg tggtgtgcgc agccagaatg atgttacgga
    11641 tgtattgcgc gttaaccgga acgtcgatat tcagcgcact ttctgccgca cgaacggaag
    11701 acagcgcgtg agtggtagta catacgccac agatacgttg cacaatcatc catgcatcgc
    11761 gcggatcgcg gtttttcacg atctcttcca tgccgcgcca catggtaccg gaagcccatg
    11821 ctttcgaaac gacgccattt tcgatttcgc aatcgatgcg taaatgcccc tcaatacggg
    11881 ttaccggatc aatagtaatt ctctggctca tgctttgctc gcctcatgac gattatgatc
    11941 gttttgtttt aaaggaggaa gtatcggcag tagacgaatg agtacgatgt aagcgcaaat
    12001 ctcaatagcc acaaaaccaa tagaaatcaa cagttcttcc caggtcggga agtaggcgta
    12061 accgccgccc gggttgaatg ccaccagcga ataggtcaga cgccaggttg cacaacctaa
    12121 cagtgcgctc agtgctgaca ggaacagcat gcgggaatca ttacgcagct tcgccacacg
    12181 cagaacgacc agcgggaaga gcatcagcag gacttcaatc cagaacatca cggagtagaa
    12241 gtcaccggca aacgctaacg acagcttgtc gcgatagata agctcgccaa agcgcagcac
    12301 gatgaaaatc gccagcaaca cactgatggt gttggtcagc ttgacgaaca gactcttttc
    12361 atccggaccg ttgccacgca gacccgcctg caccagcgaa ccttcaaaga tgacaatcga
    12421 gaagcccatg atgaacgccg tcagcagcga gaacagcggc aacatttcat agctctgcca
    12481 caacggatgc accttgtagc ccgccgagat catcagtgac cccattgaag actggtgcat
    12541 ggtcggcaac agcgcgccaa gtgcaatgat gaagaacata accttgttca gacgcttgag
    12601 agaaaccttc cagcccagac gttcaaacag tgccggagca aactccagtg ccatcacgcc
    12661 gatatagatg gtcatacaga ccgccgtctc gaacagtacc gagttcacgt tgaagtgacc
    12721 cggaatgtag aagtacggca ggttccagta gcgacccacg tcgatagtga tcgacaagcc
    12781 acccagtgag taaccaaaca gactcgccaa cagcgccgga cgcaccagcg gatggtattg
    12841 cccacggtta aagacgtata ccgcccacgc cagcgcccag ccgccacagg caaagccggt
    12901 gccaatcagc aggtcaaacg cgatccacac gccccacggg aagccgccgt tcaggtcaga
    12961 gacagagccc agaccgaaca ccagacgctt cacaatcagg agcatacaga tgacgattaa
    13021 cggtccaaaa atcatgaccg gtttactgat gattttgccg cccagcggtt gtggatcatg
    13081 actcatgatc gtctcctccg tcgtgatggt cgtttttggt gttgcgacga accagcacgg
    13141 ttaagcccgc cagcacagcc agtggtagca tcatgccttt atacagggtg tgttgaatat
    13201 tttcggaacg cgcaccggta gaaagatcgt ccagtttcgg caggtcgaga ttttcataag
    13261 gcacacccgt cagtaccaga acctgagtac cgccgccctc tttctcgccg tacagatgcg
    13321 gataatattt cggcaccgta tgcaggtaag tgtcgccaga tttcagcgtc tgacgcggat
    13381 agtggtattc gctgccaggc ttcagcgcca gacgtttttt cgcctccgcc atcagctctt
    13441 cacgcgtacc gaaaatcacc gcgcccgccg ggcacacttc tacgcagcca ggcagaccgc
    13501 ctttatcgag acgttccaca cctttctggt tgcacagctc gcacttatgc agcgcaccaa
    13561 acgggttgtt gtagtcgtac ttcggcacgt tgtacggaca ggcgaccatg cagtaacggc
    13621 aaccggtgca cacgtctttg tcgtaatgga caatgccggt tttcggatct tttttcagtg
    13681 cagagaccgg gcacacagag acacagttcg gatcgacgca gtgcatacac tgtttcttaa
    13741 tgtacgcgta gccgttctcc tcctggtctt tgttgacccc tgtgccgctg gtccacacct
    13801 gaatgatgtt attggtatac ggcgacagtt tgtcgttgtt cgaccaggtc tgttcccctt
    13861 gcgggttacg ttcagggaag ttgatatcct gacacttggt gacgcaagcc tggcagccta
    13921 cgcacaaggt cgagtcgtac aacatcccca gcgatcccgg aattggcggg cggttttcag
    13981 cagccgcatg actgacagac ggcagcgcgc ccgtcagcaa tgccccgcag gaggctgctt
    14041 taataaaatt acgtctgttc acggttattc tccccgtgag tcagcgttat ctttcttttg
    14101 ctgacgaccc agttcacgca ccgccatcac gctgacaccg gcaaccagcc caaccacacc
    14161 gccgagcaaa ccaatagcgc ctgcagagac gttgccgccc tctttagcgt taacatccgg
    14221 tttctgtgaa cgcggagtct gattttcgac gttggcaagc tgatggatgc ctttatggaa
    14281 gccgatacct tcttcgttac agccatagca tgggtgacca atcgctaccg gccacacgcc
    14341 gccaacatcg cagaattgca gcgttgagca gttgccgtaa gtttctggcc ctttacagcc
    14401 gaggtggtac aggcaccagc cttcacggtg gccttcatca ccgaactctt tggcaaaacg
    14461 accagcatcg aagtgcgggc gacgttcgca gtgttcgtga atcagacggc cataggcgaa
    14521 ggtcggacgg tttttgtcat ccagtttcgg cggtttgccg taagtgatga tgtgcgcaac
    14581 ggtcgcgagg aagttgtgcg ggttcggcgg gcagcccgga atattgataa cggttttgcc
    14641 tggcagaact tcttgcaggc tgactgcgcc agttgggtta actccagctg cggcaacacc
    14701 gccccacgca gagcaggaac cgatagcgat aatcgctgct gcgccttctg ccgctttgcg
    14761 gatgtgatcc acaatcggct caccagcaac catgcaataa ataccgttat cttttaatgg
    14821 gatggaacca tccacaacca acacatactg ccctttgtac ttctcaagtg cgttatgttt
    14881 gttctcttcg acctgatgac cgaaggcggc ggaaagcact tcgtgatact ccagagagat
    14941 agtctccagc acgaggtttt ctaccgttgg atgcgttgca cgaagcagag attccgtaca
    15001 accggtgcac tcctgcgcgc caatccagat aactggcgga cgctgcgggt tagtaaccga
    15061 ttcggccatc tctgcagcgg ctttgctact taaccccatg gtggcggcta atgctgcaca
    15121 aagcttcatg aaatcacgac ggttaatgcc gtgagaatgg atgagggtgt tatctccagt
    15181 catttatagt tattccgttg cgaagacctg gcatatattt tgcctcaatc gcaaaatcaa
    15241 taatgcgatc gatgcgccat ttaccacaca tttattatgg ttatcgatat catgatactg
    15301 cggcggggcg ctaaaacgaa gggaagaagc attagtgtaa ttaattaaag caagataata
    15361 cgtatgtttg atcaattttc gaggcgataa ccggccataa aaaagcgcaa cgtagaacag
    15421 gaattattct cacgaaagaa atccattacc atctctttat ccagtccata gcggcgcgtc
    15481 agaatacccg cttcccaggc gctaagctgg cgatctccgg tcttttcgta catccggtgt
    15541 gagtagccta atacaaaacc gcgtttataa tcggcacaga attttgtcac attaaccgca
    15601 ctgtcggcat gcgttgcttt taatcccgcc attaagcctt taccaaaatg gttattcatc
    15661 gttcacctca ttcgctatat atcgccatat atagcgatat tttaggcaaa gaacgacttt
    15721 tgcgcacgct attttttcat gcttgtttaa gacgtaatcc agtaagagat aaacctatca
    15781 ggagagatat ttatcgaacc atttcagtgt cctttgccag gcaagatcgg cggcagattt
    15841 gtcataacgg ggcgtggaat cattatggaa tccgtgatta acccccggat agatatacgc
    15901 ctcataaacc ttattattgg ctttcaacgc cgcctcgtaa gcaggccagc cctcgttgat
    15961 tcgggtgtcc agttccgcga agtggagtag taaaggcgct tcaatcttcg ccacatcggc
    16021 agtgggtgcc tgacgaccat aaaacggcac cgcgcaggcc agttccggat acgcgacagc
    16081 cgccgcgttc gatacgccac cgccatagca aaatccggta atacccactt tgcctgccgc
    16141 ttgtggatag cgttgcataa actcaattgc ggcaaagaaa tcattcatca gtttggttgg
    16201 atcaacctgc tgttgcagct cacgaccttt atcatcattt cccggataac ctccaacgga
    16261 acttaagccg tcaggtgcca gggcgatata ccccgccttc gccactcgcc gtgccacatc
    16321 ttcgatatac ggattcagtc cacgattctc atgcaccacc accacggctg gcgttttgcc
    16381 gctcattttt gcgggtttca ccagataacc ccgtacctcg ccgtgaccat ttggcgaagg
    16441 atacatgatg tactcagcaa caatctccgg gtcggtaaac tctacctgag tcgccagcgc
    16501 ataatttggc ttgagcaaat caaacaacgc taatgccgtc atcccgccca ccgcatactt
    16561 cgccgcaagg ttgaggaact cacgtttcga aattttcccg tgagcgtaat agtcgtagta
    16621 atccaacaac tcttgtggga aatctttggc ggttaaacgc ggcatcgttg cactcctcag
    16681 ttggtgtttt tttaagcaaa gcataagcac gtgtttttgc ccagtttttc gtcactctgt
    16741 gagccagact acgggatacg cgctggcgaa tcgctaaact agaaacattg tttcgaaatt
    16801 gaacggtgga aaggagaggt catggtctgg ttagcgaatc ccgaacgtta cgggcagatg
    16861 caataccgct attgcggaaa aagcggttta cgcctgcccg cgttatcgct cggtttatgg
    16921 cacaatttcg gtcacgttaa cgcgctggaa tcacagcgtg cgatcctgcg taaagcgttt
    16981 gatttgggca ttacgcactt tgatttagcc aacaattacg ggccgcctcc aggaagcgca
    17041 gaagagaact ttggtcgcct gctgcgggag gattttgccg cttatcgcga tgaactgatt
    17101 atctctacca aggctggcta cgatatgtgg cccggccctt acggctctgg cggttcacgt
    17161 aaatacctgc tcgccagcct cgaccaaagc ctgaagcgta tggggcttga gtatgtcgat
    17221 atcttttact ctcatcgcgt cgatgaaaat acgccgatgg aagaaaccgc ctctgcgctg
    17281 gctcatgcgg tacaaagcgg taaggcgctg tatgtcggga tctcctctta ctcgccagag
    17341 cggacgcaaa aaatggtcga gttgctgcac gagtggaaaa ttccgctttt aattcatcaa
    17401 ccttcgtaca atttactgaa ccgctgggtg gataaaagcg gcctgctgga taccctgcaa
    17461 aataacggcg tgggctgtat tgcctttact cctctggctc agggattgct gaccggaaaa
    17521 tatctcaacg gcattccgca agattcacgg atgcatcgtg aagggaataa agttcgtggt
    17581 ctgacgccga aaatgcttac cgaagccaac ctcaacagcc tgcgcttatt gaatgaaatg
    17641 gcacagcagc gtggacaatc aatggcgcaa atggcgttaa gctggttgct gaaagatgat
    17701 cgcgtgacgt cggtattgat tggtgccagc cgcgcggagc aactagagga gaacgtgcag
    17761 gcgctgaata atctgacatt tagcaccgag gagctggcgc agattgatca gcatatcgcc
    17821 gatggcgagc tgaatctgtg gcaggcgtcg tccgataaat gacctgttaa taacgggcgc
    17881 gcgaaccgcg cccgaatcag atcagtgatt atgacgagtc agtcggtcaa gatagcccat
    17941 cacaaatgca gaaagcacaa acgtcagatg gataatgacg taccacatca gtttgttatc
    18001 cgggacattt ttcgcatcca taaagacgcg cagtaagtga atggaagaaa ttgccacaat
    18061 cgacgctgct actttgtttt tcagcgacgt tgcgtccatt ttccccagcc agttcagctt
    18121 ctctttgttc tcggagatat ccagctgtga gacgaaattc tcataaccgg aaaacatcac
    18181 catcaccagc aaaccgccaa ccagcgtcat atccaccagc gacagcaaca cgaggatcaa
    18241 atctgattcc gccatcgaga agatattcgg cagtacgtga ataatctcct ggaagaactt
    18301 cagcgccagg gcaactaacg ccagcgaaag gccaaagtac acgggggcaa gcagccagcg
    18361 agaagcatac attgcatttt caagaaaacg ttccatacat tcctgtcgtg tagttaaaac
    18421 gggcaacagt atatcgcaat tgagtaaaca gcacgcaaca gcaaatcaac atatacgtta
    18481 aacctttgtc gcataattct gaggtttagg gatcttcctg gttcccttat caacgcatcg
    18541 agagcgtcta cccttaaagg acgttgaacc tgaagggaga aaaacgatgt ctcatttaaa
    18601 agacccgacc acgcagtatt acactggtga atatcccaaa cagaaacaac cgacgccagg
    18661 catccaggcg aagatgacac cggtaccgga ttgcggcgag aaaacctatg ttggtagcgg
    18721 tcgcctgaaa gatcgtaaag cactggtgac agggggcgat tccggaatag gtcgcgctgc
    18781 cgccatcgct tacgcgcgtg aaggggctga cgtggcgatc agttatcttc ccgtggaaga
    18841 agaagacgct caggatgtga aaaagatcat tgaagaatgc ggacgcaaag ccgttctgct
    18901 gccaggcgat ttaagcgatg agaaattcgc ccgttcgctg gttcacgaag cgcacaaggc
    18961 gttaggcggg ctggatatta tggcgctggt cgccgggaaa caggttgcca ttccggatat
    19021 tgcagacctc accagcgaac agtttcaaaa gacctttgcc attaacgttt tcgcgctgtt
    19081 ctggctaacc caggaagcga tccccctgct accgaaaggt gcaagtatca tcaccacttc
    19141 gtcaatccag gcataccagc caagtccgca tttactggac tatgcggcta cgaaggcggc
    19201 gattctgaac tacagccgtg gcctggcaaa acaggtcgcg gagaaaggta ttcgggtgaa
    19261 tattgtcgcg ccaggcccta tctggacggc actgcaaatt tccggcggac aaacgcagga
    19321 taagatcccg cagtttggtc agaaaacgcc gatgaagcgt gcggggcaac cggcggaact
    19381 ggcccctgta tatgtttatc tggcaagtca ggagtcgagc tacgtcaccg cagaagtgca
    19441 cggcgtgtgc ggcggcgagc atttaggtta aaaaaatgcc cggttgtgaa aagcaaccgg
    19501 gcatcattgt gaattcactc ttacctgatg tggcgaaaac gccttatccg gcctacaaac
    19561 gcacgaagat tcaacatgtt gcaatagatt ttgtaggcct gatgagcgta gcgcatcagg
    19621 caattctact ttacttcgct ttggcggttt cttcgccgac cagacctatc ttcaggtaac
    19681 ccgcctgatg cagcgtatcc attaccttca tcaacgtctc gtaatcgacg gttttgtccg
    19741 ctcggaagaa gatggtggtg tctttcttgc cttcggttaa cgcattcaac gccgtaatca
    19801 ttgtttcatc ggtgaccgga tcgttaccga taaacatcga gttgtctgcc ttcaccgaca
    19861 gataaaccgg tttttccggg cgcggctgcg gcgtgctggt agaagcaggc aagttcacct
    19921 tcacatctac cgtcgctaac ggtgccgcca ccataaagat aatcagcaga accaacatca
    19981 cgtcgataaa cggcgtcacg ttgatatcat gcatttcgcc gttatcgtcg aggttttcgt
    20041 taagatgcat tgccatcgga cattatcctg cgcgtaattt ttgtgcgaca cgaaccggat
    20101 gcgcagcggc gctggcttcc agatccaggt cacggctttg cagcaacaat acctgcgctg
    20161 caacatcacc cagcatcgct ttaaagccgc caatctggcg tgcaaatacg ttatagataa
    20221 cgaccgcagg aatcgctgcc acgaggccga ttgccgttgc taacagagct tctgcgatac
    20281 ccggcgcaac gactgccagg ttagtggtct gcgtttgcgc gataccaata aagctgttca
    20341 tgatgcccca gaccgtacca aacagaccaa cgaacggaga aatcgcgccg atggttgcca
    20401 gatagccgtt accgcggccc atttgacgac ccactgcggc gacccgacgc tccagacgga
    20461 agctggtacg ttctttaata ccttcgttat cgtcgctgcc ttctgacagt tccagctcat
    20521 tctgcgcttc attgagcaaa tgcaggctta agcttttgct accaaaatca gcggcgatat
    20581 cgttggcctg gtttaaggaa cgcgcttcag ccagcagttg ctgctcgcgc ttaaggcgac
    20641 gcttctgatt gaagaactct acgctcttac tgaagaagat tgcccaagtg actacggagg
    20701 ccaaaataag cccaatcatc acgcacttaa cgacaatatc ggcgtgctga tacatacccc
    20761 agacggaaag gtccgtctgc atcaaattat tacccactgt gtatctccag gacgcaagtc
    20821 acaaaatctg cgcataataa tatcaaaatg acgtcgaatt gatagtcgtt ctcattacta
    20881 tttgcatact gccgtacctt tgctttcttt tccttgcgtt tacgcagtaa aaaagtcacc
    20941 agcacgccat ttgcgaaaat tttctgcttt atgccaattc ttcaggatgc gcccgcgaat
    21001 attcatgcta gtttagacat ccagacgtat aaaaacagga atcccgagat ggcggacaaa
    21061 aagcttgata ctcaactggt gaatgcagga cgcagcaaaa aatacactct cggcgcggta
    21121 aatagcgtga ttcagcgcgc ttcttcgctg gtctttgaca gtgtggaagc caaaaagcac
    21181 gcgacgcgca atcgcgccaa tggcgagttg ttctatggac ggcgcggaac gttaacccat
    21241 ttctccttac aacaagcgat gtgtgaactg gaaggtggcg caggctgcgt gctatttccc
    21301 tgcggggcgg cagcggttgc taattccatt cttgcttttg tcgaacaggg cgatcatgtg
    21361 ttgatgacca acaccgccta tgaaccgagt caggatttct gtagcaaaat cctcagcaaa
    21421 ctgggcgtaa cgacatcgtg gtttgatccg ctgattggtg ccgatatcgt taagcatctg
    21481 cagccaaaca ctaaaatcgt gtttctggaa tcgccaggct ccatcaccat ggaagtccac
    21541 gacgttccgg cgattgttgc cgccgtacgc agtgtggtgc cggatgccat cattatgatc
    21601 gacaacacct gggcagccgg tgtgctgttt aaggcgctgg attttggcat cgatgtttct
    21661 attcaagccg ccaccaaata tctggttggg cattcagatg cgatgattgg cactgccgtg
    21721 tgcaatgccc gttgctggga gcagctacgg gaaaacgcct atctgatggg ccagatggtc
    21781 gatgccgata ccgcctatat aaccagccgt ggcctgcgca cattaggtgt gcgtttgcgt
    21841 caacatcatg aaagcagtct gaaagtggct gaatggctgg cagaacatcc gcaagtagcg
    21901 cgagttaacc accctgctct gcctggcagt aaaggccacg aattctggaa acgagacttt
    21961 acaggcagca gcgggctatt ttcctttgtg cttaagaaaa aactcagtaa tgaagagctg
    22021 gcgaactatc tggataactt cagtttattc agcatggcct actcgtgggg cgggtatgaa
    22081 tcgttgatcc tggcgaatca accagaacat atcgccgcca ttcgcccaca aggcgagatc
    22141 gattttagcg ggaccttgat tcgcctgcat attggtctgg aagatgtcga cgatctgatt
    22201 gccgatctgg acgccggttt tgcgcgaatt gtataacatt gccacttttg gacaattttg
    22261 cagacattta attgtgaaaa gtcttaaatt gttgcgtccg ggatcaaggc gtcccggacg
    22321 aatcaggagt acaataggca gataaaggct taaacgctgt tccacaggaa agtccatggc
    22381 tgttattcaa gatatcatcg ctgcgctctg gcaacacgac tttgccgcgc tggcggatcc
    22441 tcatattgtt agcgttgttt actttgtcat gtttgccacg ctgtttttag aaaacggcct
    22501 gctgcccgcc tcatttttgc caggcgacag cttgttgata ctggcaggcg cattgattgc
    22561 ccaaggggtt atggattttc tgcctacgat tgcgattctg accgccgcag caagtctggg
    22621 ctgctggcta agttatattc aggggcgctg gttagggaat accaaaacgg tgaaaggctg
    22681 gctggcacag cttcctgcta aatatcacca gcgcgccacc tgcatgtttg accgccacgg
    22741 tctgctggcg ctgctggctg gacgttttct tgcatttgtc cgtacgctgc tgccaaccat
    22801 ggcgggaatt tccggtctgc caaaccgccg cttccagttt ttcaactggt taagtggatt
    22861 gctgtgggtc agcgtggtaa ccagttttgg ctatgcctta agtatgattc cgttcgttaa
    22921 acgccatgaa gatcaggtaa tgacgttcct gatgatcctg ccaattgcct tgttaaccgc
    22981 tggcttgtta ggcacgctgt ttgtagtgat taaaaaaaaa tactgtaacg cctgacgatt
    23041 ttccccgttc ccggctgctg taccgggaac gtatttaatt cccctgcatc gcccgcattc
    23101 ttgccgcatc ttcccccggc gtcacaccga agtaacgttt aaactcacgg ctaaattgca
    23161 atgcgctttc atagtcgacg cgcatcgctg ctgcgctggc cttcatgccg tcatggatga
    23221 tcatcatccg cgccttatgc agacggtaat tcttcaaata ctgcaacggc gaggtgctgg
    23281 tgacagactt aaaattatga tggaacgccg atacgctcat gttggcttct gccgccagtt
    23341 gctcgacgct caggttttcg gtgtatttat tctcaatccg tttcagcacg cggctaatca
    23401 gactgaagtg agtctggcga ctgaccagcg ccagtaacgc gccgccgcaa ggtccggtca
    23461 gcacgtagta cagaatttcg cggatgatct gtttgccgag aatacgcgca tccagtggtc
    23521 gctccatcac gtcgagtaac cgctccgccg cgcataaaat ctcttctgat aacgtggcgg
    23581 agttaatccc gctggctgcc atcgacggct ggaaatgctc atcttcgcca atgtccatca
    23641 acagttcctg taactgcaaa atatcgacat tgagacgcaa tcctgccagc ggcacctctg
    23701 acgtggcata ggtttcgcac tcaaacggca acggcaccgt cagcagcagg tattcattgg
    23761 catcataacg aaacacgcgt tcattgatat aaccgatttt atgcccggaa aagagaatta
    23821 tgatgccagg ctcgtacatc accggtgtac gtgcgaaagg cgtctcgcca tacaacaaac
    23881 gcacatcggg caacagttct gacaaactat tttctttatt tttcagttta ttaactttat
    23941 ccgccagcaa gcggcaaatc tcttcacgtt tcatatcgcg taatttctta ggaataatgc
    24001 ggcaatttga ttgtgcgcaa ttttgtagca tttctccagc actctggaga aataggcaag
    24061 acattggcag aaatgagcat tgagagccag ggcgctggcg atcacaatga aaaacatcag
    24121 gcagatcgtt ctctgccctc atattggccc agcaaaggga gcaagtaatg aacaacttta
    24181 atctgcacac cccaacccgc attctgtttg gtaaaggcgc aatcgctggt ttacgcgaac
    24241 aaattcctca cgatgctcgc gtattgatta cctacggcgg cggcagcgtg aaaaaaatcg
    24301 gcgttctcga tcaagttctg gatgccctga aaggcatgga cgtgctggaa tttggcggta
    24361 ttgagccaaa cccggcttat gaaacgctga tgaacgccgt gaaactggtt cgcgaacaga
    24421 aagtgacttt cctgctggcg gttggcggcg gttctgtact ggacggcacc aaatttatcg
    24481 ccgcagcggc taactatccg gaaaatatcg atccgtggca cattctgcaa acgggcggta
    24541 aagagattaa aagcgccatc ccgatgggct gtgtgctgac gctgccagca accggttcag
    24601 aatccaacgc aggcgcggtg atctcccgta aaaccacagg cgacaagcag gcgttccatt
    24661 ctgcccatgt tcagccggta tttgccgtgc tcgatccggt ttatacctac accctgccgc
    24721 cgcgtcaggt ggctaacggc gtagtggacg cctttgtaca caccgtggaa cagtatgtta
    24781 ccaaaccggt tgatgccaaa attcaggacc gtttcgcaga aggcattttg ctgacgctaa
    24841 tcgaagatgg tccgaaagcc ctgaaagagc cagaaaacta cgatgtgcgc gccaacgtca
    24901 tgtgggcggc gactcaggcg ctgaacggtt tgattggcgc tggcgtaccg caggactggg
    24961 caacgcatat gctgggccac gaactgactg cgatgcacgg tctggatcac gcgcaaacac
    25021 tggctatcgt cctgcctgca ctgtggaatg aaaaacgcga gaccaagcgc gctaagctgc
    25081 tgcaatatgc tgaacgcgtc tggaacatca ctgaaggttc cgacgatgag cgtattgacg
    25141 ccgcgattgc cgcaacccgc aatttctttg agcaattagg cgtgccgacc cacctctccg
    25201 actacggtct ggacggcagc tccatcccgg ctttgctgaa aaaactggaa gagcacggca
    25261 tgacccaact gggcgaaaat catgacatta cgttggatgt cagccgccgt atatacgaag
    25321 ccgcccgcta agctttttac gcctcaaact ttcgttttcg ggcatttcgt ccagacttaa
    25381 gttcacaaca cctcaccgga gcctgctccg gtgagttcat ataaaggagg aacgtatggc
    25441 taatccaacc gttattaagc tacaggatgg caatgtcatg ccccagctgg gactgggcgt
    25501 ctggcaagca agtaatgagg aagtaatcac cgccattcaa aaagcgttag aagtgggtta
    25561 tcgctcgatt gataccgccg cggcctacaa gaacgaagaa ggtgtcggca aagccctgaa
    25621 aaatgcctca gtcaacagag aagaactgtt catcaccact aagctgtgga acgacgacca
    25681 caagcgcccc cgcgaagccc tgctcgacag cctgaaaaaa ctccagcttg attatatcga
    25741 cctctactta atgcactggc ccgttcccgc tatcgaccat tatgtcgaag catggaaagg
    25801 catgatcgaa ttgcaaaaag agggattaat caaaagcatc ggcgtgtgca acttccagat
    25861 ccatcacctg caacgcctga ttgatgaaac tggcgtgacg cctgtgataa accagatcga
    25921 acttcatccg ctgatgcaac aacgccagct acacgcctgg aacgcgacac acaaaatcca
    25981 gaccgaatcc tggagcccat tagcgcaagg agggaaaggc gttttcgatc agaaagtcat
    26041 tcgcgatctg gcagataaat acggcaaagc cccggcgcag attgttatcc gctggcatct
    26101 ggatagcggc ctggtggtga tcccgaaatc ggtcacacct tcacgtattg ccgaaaactt
    26161 tgatgtctgg gatttccgtc tcgacaaaga cgaactcggc gaaattgcaa aactcgatca
    26221 gggcaagcgt ctcggtcccg atcctgacca gttcggcggc taacatgcaa attctcccgg
    26281 tgacggtaat gttccgctac cggacttttc agaaatcatt tattcccctc gcgtcccgcc
    26341 cgttgttact cttccttgtt caggaatgcc aaatataagg acatcatcat gcagagccgg
    26401 aagctcttaa aagaacaact catctatatc cgggataaac gcaacggaga ggtgaaaaac
    26461 agatgaaaat aatacttctg tttttagcag ccctggcaag ttttaccgta cacgcgcagc
    26521 ctccttcaca gaccgtagaa caaacagtcc ggcagattta tcagaactat aaatcagatg
    26581 ccagtacacc ttatttcggt gaaaccggag agcgggcgat aacttcagcg cgtattcaac
    26641 aggcgcttac cctgaacgac aatcttacgc tgccgggcaa tattggctgg ctggattatg
    26701 atccggtttg tgattgtcag gattttggcg atctggtgct agaaagcgtt gcgataaccc
    26761 aaactgacgc cgatcatgcc gatgccgttg tgcgctttcg tatctttaaa gatgataaag
    26821 aaaagaccac gcagacactg aaaatggtgg cggaaaatgg tcgttgggtc attgacgata
    26881 ttgtcagcaa tcatggcagc gtcttacaag cagttaatag cgagaatgaa aaaacgctgg
    26941 ccgctttagc ttcgttgcaa aaagaacagc cggaagcctt tgttgccgaa ctctttgaac
    27001 atattgctga ttatagctgg ccgtggacgt gggtggtttc cgactcttac cgccaggcgg
    27061 ttaatgcctt ctataaaacc accttcaaga cggccaataa tcccgatgaa gatatgcaaa
    27121 tagaacggca atttatttac gacaatccga tctgttttgg cgaagagtcg ctattttcac
    27181 gcgttgatga aattcgagtc ctggagaaaa ccgccgattc cgcccgcatt catgttcgtt
    27241 ttacgctgac caatggcaac aacgaagagc aagaactggt tttacagcgg cgcgaaggca
    27301 agtgggaaat cgctgatttt atccgcccga acagcggcag cctacttaag cagattgagg
    27361 caaaaaccgc cgccagatta aagcaatgag ctgaattaaa taacaattag ccggaacaat
    27421 aaataaaagg gaacactata tgaaaacgat tttcaccgtg ggagctgttg ttctggcaac
    27481 ctgcttgctc agtggctgcg tcaatgagca aaaggtcaat caactggcga gcaatgtaca
    27541 aacattaaat gccaaaatcg cccggcttga gcaggatata aaagcactac gcccacaaat
    27601 ctatgctgcc aaatccgaag ctaacagagc caatacgcgt cttgatgctc aggactattt
    27661 tgattgcctg cgctgcttgc gtatgtacgc agaatgataa aaaaatcccc ggcagcatgt
    27721 cagttgccgg ggattttttt taacgtccaa ccgccgcttt agggcgtttc ttcgcaccag
    27781 cattcaccgg acgagattgc gtagacgacg ctttttttgc cgtagcaggc gtctgacgct
    27841 gggtcgccat cggcgtatgt ttagtcaacg ccggacgggt attgcggttc tggcgacgag
    27901 cttcacgcat ctcttcaatg gttggcgcag gcactaagca atcgcgacgg ctgccaatca
    27961 gatgcttttt gcccatcgct tccagcgccg ggcggattaa cggccagttt gccggatcgt
    28021 ggtaacgcaa caacgcttta tgcaaacgac gctgtttgtc gcccttcggt acgaagacgt
    28081 cttcgctctt ataaccaatc ttcgccagcg gttttttccc ggtgtaatac atggtggttg
    28141 agttcgccag cggcgatggg tagaagttct gtacctggtc gagacggaaa cgatgctttt
    28201 tcagccacag cgccaaattc accatatctt catcacgcgt accggggtgc gcagagatga
    28261 aatacgggat cagatactgc tctttacctg cctgtttcga gtaggtatcg aacagctctt
    28321 taaagcggtc atagctgccc atgcccggct tcatcatctt cgacaacggc ccttcttcgg
    28381 tatgttccgg agcaatcttc agataaccgc cgacgtgatg ggtcgccaac tctttgatat
    28441 agcgcggatc ttccacggcg atgtcataac gtacgcctga ggcgatgagg atttttttaa
    28501 tgcctttcag atcacgcgca cggcgataga ggttgatcgt cggttcgtgg ttagtgtcca
    28561 tgtgcggaca aatatccgga taaacgcacg acaaacggcg acaagtttgt tcagcgcgtg
    28621 gcgatttgca gcgcaacata tacatgttgg cagttggccc accgagatcg gaaatcacgc
    28681 ccgtaaaacc tggaacggtg tcgcggatcg cttcgatctc attaatgatc gaatcttccg
    28741 aacggctctg aataatgcgc ccttcgtgct cggtaataga acagaaagaa cagccgccaa
    28801 agcagccgcg cataatgttg accgaaaaac ggatcatttc gtaagccgga atacgggcat
    28861 tgccataggc cggatgtggc acgcgcttgt acggcagcgc aaaaacgctg tccatctctt
    28921 cggtagaaag cgggatagca ggcgggttga tccacacata gcggtcaccg tgtttttgca
    28981 tcaatgcgcg gacgcagcct gggttggttt cgtggtgcag aatacgcgaa gcatgggcgt
    29041 acagcacttt atcgcccttc actttctcga aagaaggcag caacacgtag gttttttccc
    29101 acggtttcgg gcgcggtggc tgcacggtta cggctttggc ttcctgcttt ttcggtgcca
    29161 ccggtttgtt atccgcgcac ggcaaatctt caccatacgg atgcgggatt gggtcgattt
    29221 ttccaggggt atcaagacgg gtggaatcca cgccgctcca gccaggcagc gcctctttca
    29281 cgataatcgc ggtattacgc acatcgcgga tttcactaat cggctcgccc atcgccagac
    29341 ggtgcgccac ctccaccagc ggacgctcac cgttaccaaa catcagcatg tcggctttcg
    29401 aatccaccag cacggaacgg cgcacggtat cggaccagta atcataatgt gcggtacggc
    29461 gcagactagc ctcaataccg ccgaggatca ccggtacatc tttccacgcc tctttacaac
    29521 gctgggtata aaccagtgtg gcgcgatccg ggcgcttacc cgcgacgtta tccggcgtgt
    29581 aggcatcgtc atgacgtaaa cggcgatcgg cggtataacg gttgatcatc gaatccatgt
    29641 tgccagcagt aacaccgaaa aacagattcg gtttacccag acgcataaag tcgtctttgc
    29701 tgctccagtc cggctgggcg atgatcccga cgcgaaagcc ctgtgcttcc agcatacgac
    29761 cgcaaatcgc catcccgaag cttgggtgat cgacatacgc gtcgccagta accaaaatga
    29821 tgtcgcagct atcccagcca agttgatcca tctcttcacg agacatcggc aaaaacggtg
    29881 ccggtccaaa acaggcggcc cagtactgcg gccaggagaa caggtcgcga tccggttgga
    29941 tcagggagat agagctcatt ttgcttccag aaatgataaa aaaataatca aaggccgggg
    30001 attataagcc ggaacgaaag agaaatcgaa aagtattcca tactcgccct cctcgggcga
    30061 gtatgaagat tacggtaccg gattgactaa cagttgccca atcgagccac ggtccgccat
    30121 ttccagcgtc tgactgttga agtagaacgg gaagtgcgcc caggaaggct gaccgaaata
    30181 aacaagcagc tccacttgtc cgtcaaccca aacggtatct ttccagcctc tgtcttccgg
    30241 gaacggcatc gcaccattca cgttacggat ctggaacatc acgccttcaa tatggaacgc
    30301 ttgcggctcg tccgcgcgta ccgtccagcg ttcccacgtt ccttgctgcg cggtgacatc
    30361 aatacggttg acgtcccaca gctgtccatt aatacccggg tcatcaccca gactgatatc
    30421 gcgactgcga atcggcgaac cggccatgat ttcagttggc agcaagcgca tcggaagact
    30481 gtctgtgacc agcggcagaa ggccggttgg gcgcagcgtt agcaccaggg tagaaaccag
    30541 aatactggat ggctcaaaga agccgcgaat acgatcaaca atgctcgccg cttcgccaca
    30601 ggtgatcgac acttcatcgc cgttgctcat atccaccaga atctcgcggc gctcgcccgg
    30661 tgccagcgaa agttgcttca ccgatacagg agcaggcagg aatccctgat cgccagaaat
    30721 cacatgtaac gggcgaccat cgctcatctg tagttgatag cgacgagagt tcgacgcgtt
    30781 cagcagtcgc aagcgcaccc agccacgcga gacttcaacg tacgggcttt gtacaccgtt
    30841 aaccagcagc gtatcaccaa caaagccgcc gcttcccggt tcgttgtatt ctggcgtacc
    30901 aaagttatcc agccgtttat cctggataat gaccggaaaa tcatccacat cataatggtt
    30961 ggggataggc agcgacttgc tgacttcatc ttccaccagc cacattccgg caaggccgtt
    31021 atagacctgc tgagccgtgc ggttgggagt attggcgtga taccacagag tagctgcgtt
    31081 ctggcgaata ggcagtacgg gtgcccagtc agcgtttggc gacatcatcc gtgccggacc
    31141 gcccatcagc gggccaggta cctgtagccc ggccaccgtc attgagacat tttctgtcag
    31201 gcggttgctg taaataagct taacatcgtc gcccttccag acgcggatag tcggccccag
    31261 gtaacgacca ttgattcccc agaccgacgc gcgtgtccct ggcgtaaatg accagtgcgc
    31321 acgttgtaca gtcataaaca gcggttgccc acggcgagat tcaagtagcg gcggaacggg
    31381 tagcggttgc tgttgcccgg ctgcgctggc cttcagggga acagcgcctg cacaaagtgc
    31441 aatccccgat gcctgaatga actgacgccg actgagtgac atatttgctc catgtaaaac
    31501 tggctaaagt aataccgtgc aggaaagtgt tccccgcaaa aatacagatt gccgacttaa
    31561 acttttccgg cggcttcgcg ttctgcgact tctttatcga gctcggcgat tttttgttcc
    31621 attatcgaac gacaatgggc agccagctca cgaacctgat ctttgccata ctgactgacg
    31681 tcaattggcg gcagcatttc gacaatcacc agaccgttgt gcagtcgatt aagattaatc
    31741 ttattcgaag ttgtagagac gcacacggga ataatcggga cgcccgccgc aattgccgcg
    31801 tgaaatgctc cagtcttgaa cggtagcagg ccgcgaccac ggctgcgggt tccttccggg
    31861 aacatccaga tggaaatacg gcgttttttg aaatgattca cgacttccgc aatggtgccg
    31921 tgagctttag tgcgattgtt tctgtcgatc aataagttgc cggttaacca gtacaactgc
    31981 ccgaagaagg ggatccacag caagctcttt ttacctaccg tcaccgtcgg cggttgcaca
    32041 atgttcgatg ctgtcaccat gtcatagttg ttctggtggt tagcgatata gatagcattg
    32101 ccgtagcttt cagcatctgc aggtttgcgg cactcaactt tcaggccgaa cagcggcgca
    32161 agacggccaa acatatgccc aaaggtggcc acatgtttcg ggttacgcgg gctgaaaagg
    32221 cagtaaatgg agccgaatac acagactaag atgctgtaaa tcacggtaat aataagacga
    32281 aagatatata gcataacacc ctctgaaggt ctgagagact ggcattgtac gtcacctgaa
    32341 atcaggttag ggcattgtta aaagccgctc tctggaaaag cgtattgtta atcgcaacgc
    32401 atgaataaac aacggtttca agggaatttt tatcgtttcc gggggttgaa tacgctgccg
    32461 gatgacgact taacgtttca tccggcgttc cttgcaagcg ggaggaaaca ggccctcccc
    32521 ggcatattac tcttcgctat caccgttgct ggcacggcga ggagagtcga tctcaacacg
    32581 atcgatacgc tgcaaaccgc gcatcaacgt accgcggcgt ccacgttcac cagtgacttt
    32641 ctgtaactct tccgggcgca gtttaatttt gcgtttccca acatgaatgg tcagcgtgct
    32701 ttgcggcggc agaacgtaca attgcgccag accatcttct ccacgcgcgg cttctgccga
    32761 tggaatgttg ataatcttgt tgcctttgcc cttcgacagc tgcggcagat cgcttaccgg
    32821 gaacatcaac atacggcctg cctgagtgat tgccagcagc atatcggaag catcttcaat
    32881 caccaccggc ggcataacat gggcattttc cggtaaggtg atcaaagcct tacctgcacg
    32941 gttacgcgcc accagatcgt taaaggtgca gacgaaaccg taacccgcat cggaagccat
    33001 cagcagtttc tgatcgtcgc tttccatcag catatggtca acggtcgccc caggcggcaa
    33061 cgttaatttg ccggtgagtg gttcgccctg accacgcgcc gacggcagcg taatcgggtc
    33121 gatggcatag ctacgaccgg tggaatcaac aaacactacc gattggttgc tcttaccttt
    33181 caccgccgct ttgaagctat cacccgcttt ataattcagg cccggcgcgt cgatatcatg
    33241 gcctttagcg ctgcgtaccc agcccatctg cgacagcaca atggtgacag gttcagacgg
    33301 cagcatgtcg tgctcgctca tcgctttcgc ttcttcgcgt tcctgcaacg gcgaacgacg
    33361 atcgtcaccg taggcttgcg cgtctgcctg cagttctttc ttcagcaggt tattcatttt
    33421 acgctcggaa gccaaaatgc cctgcaactg gtcgcgctct ttttccagct cactctgctc
    33481 accgcgaatc ttcatctctt ccagtttggc aagatgacgc agtttcagtt cgaggatcgc
    33541 ttccgcctgg gtttccgtaa ggccaaaccg cgacatcagc gccggtttcg gttcatcttc
    33601 gttacgaatg atctcaatca cttcgtcgat gttgagaaac gccaccagca aaccttccag
    33661 gatatgcagg cgcttgagga ctttctccag acgatagttc agtcggcggc gcacggtatc
    33721 gcgacggaac accagccatt cggagaggat ttccagcagg tttttcaccg ccggacgacc
    33781 atccagaccg atcatattga ggttaatgcg atagctcttt tccagatcgg tggtagcgaa
    33841 gaggtggttc atcacctgat ccatatccac gcggttggaa cgcggcacaa tcaccagacg
    33901 ggtcgggttc tcgtggtcag attcatcgcg cagatcgtca accatcggca gctttttgtt
    33961 gcgcatttgc gcagcaattt gctccagtac acgcgcacct gaaacctgat gcggtaatgc
    34021 gctgataacc accgcgccat cttctttctt ccacaccgcg cgcatacgca ctgaaccacg
    34081 tccgttctcg tagattttac ggatctcggc gcgcgaagtg ataatttccg cttcagtcgg
    34141 ataatccggc ccctgcacga tatccagcag ctgatcgagc gtggttttcg gctggtcgat
    34201 taatgcgatt gccgcctgag ctacttcacg caggttatgc ggtggaatat cggtcgccat
    34261 gccgacggca ataccggtgg tgccgttaag caaaatgttt ggcagacgag caggcagcat
    34321 tttcggctcc tgcaaagtgc cgtcgaagtt tggcacccag tcagccgtcc cctgccccag
    34381 ctcgctcaat agcagctcgg aatatttcga caaccgggat tcggtgtaac gcattgccgc
    34441 gaacgatttc ggatcgtccg gcgcgcccca gttcccctga ccatcaacca gcggataacg
    34501 gtaagagaac ggctgcgcca tcaggaccat cgcttcataa caggcgctat cgccgtgcgg
    34561 atggtattta cccagtacgt caccgacggt acgggccgat tttttaaatt tggcgctggc
    34621 attcaggccc agttcagaca tcgcatacac aatgcggcgc tgaacaggtt tcagaccatc
    34681 accaataaac ggcaacgcac ggtccatgat cacgtacatg gagtagttta agtaggcgtt
    34741 ttccgtaaat tcatgtagcg caaggcgctc tgccatatcg ctcattaatt ctgattcctc
    34801 aactgattcg ccagccttca aaggggcaat attgccggag atactacatt atctgccgcg
    34861 tcgagtcaca ggagatcagc gtgaatagcg ccgccggacg tgctccgacg gcgataaagg
    34921 catcaatgtg ccttgatata caactctttg ctgtagactt gatcggcata gagggcagca
    34981 caaggttgcg cccatgccac accactcggc atgggggtcc gctccgggcg attcgagaag
    35041 ttgaggttat gcgagtccag ggaagcctaa agcatcaaag tagtcgactg taagtatatg
    35101 gttcagccca gtttagctgt gtctccacca gtgccccgcg cctcctgcca taagtgcggc
    35161 ttgctcatgg gtacccccga gacggcttac ggcgcaccgg ataataaatt tcgcgtatac
    35221 atggtattcc ttaccggaga aattataagc ccaacaacaa tacgcctgtg gcattgttcg
    35281 ctcaaactta tcgcgagtga tttggctcac actccggtaa agattcccca tcacgctaac
    35341 ggcacataaa catccgtttc cagtcgctgc tccgtcaccc cttcggcaag attggtgtag
    35401 tgaaacagaa tcggtgcttt acgcattttc tcgccgcttg caggcagcca gtggcgaata
    35461 atgccccata tcgtgtgtga aatatcgtcc agctcgccaa cgtggcgggc cacggcataa
    35521 cgtccaccgg taagttcacc attgctcaca ccataacgat tatcgggaat cggttcgcta
    35581 acgctgccgc agatatcaaa gcgaaacgct tccggtgcgg tggtggcagg gtcgtcccag
    35641 gcgacgccaa aagtctggct ttggttaaca ggagaaagcc ccgtctcttt acgccacatg
    35701 ataaatttcg ccacactgta gttgagcaac tcagggctac agcggtgcgg caacatcgcc
    35761 acagggattg atgggaaatc gataatgttt acatccagtg tcaggtttgt cattttgcct
    35821 ccctgcgaaa ttattcccgg gaacgcgtcc cgggagcggt aacaattacg gatttacttt
    35881 gcggatctgt ttgacgtcaa tttcaacaga attccagtct ttatcgactt caccctgaat
    35941 ctcaaccgta tctttcggcg tcaccgtcac accgttccag cgtttgtggt cgatatcaac
    36001 attgatagta ccgctggcat ctttaaacac gtagagatcg tcagagatgc gttcaacgat
    36061 attgccgcgc agggttaccc aggtgtcgtc acgcagggat tttgcgcttt ctacagtcgt
    36121 tacgctgccg ttcggcccct ggaatcctcc ggcctgactt tgcgttgccg atgggccaga
    36181 aaaaccgccc tgctctgctg ccatcaccgg tgcactgcac agggccatta ctgcgattac
    36241 tgctgcgaat tttttcatgt ttattactcc ctttaatgtc tgtttccgag catttaacaa
    36301 gatagtcctt aacaacttct taagggaaaa aaataaaatt taatgctgta cagagcgcgt
    36361 tacaacacgg tttactggca gcaaatacgg ttatcgcagg gatgaaaaaa tgcgaatttt
    36421 actgatagaa gatgacatgc tgattggcga cggcatcaaa actggcctta gtaaaatggg
    36481 ttttagtgtc gactggttta cacaaggtcg tcagggaaaa gaggcgcttt atagcgcacc
    36541 ttatgatgcg gtgatcctgg atttaacctt accaggcatg gatggtcgcg atattttgcg
    36601 cgaatggcga gagaaaggtc agcgtgagcc ggtactgatc ctgaccgcgc gtgatgcgtt
    36661 agcggaacgt gtagaagggc tgcgtctggg agctgacgat tatctgtgta aaccttttgc
    36721 gttgatagaa gtcgccgcca ggctggaggc tctgatgcgc cgaaccaacg gccaggccag
    36781 caacgagctg cgccacggta acgtcatgct cgaccccggc aaacgtatcg ccacgctggc
    36841 tggcgaaccc ttaacactga aaccaaaaga atttgccctg ctggaattac tgatgcgtaa
    36901 cgctggtcgg gtactgccgc gcaaactgat tgaagagaaa ctgtatacct gggacgaaga
    36961 ggtcaccagt aatgccgttg aagtgcatgt gcatcatctg cgacgcaaac tcggtagtga
    37021 ttttattcgt accgtgcatg gtattggcta cacattaggt gagaaatgaa atttacccaa
    37081 cgtcttagtc tgcgcgtcag gctgacgcta atctttttaa ttctggcctc ggtgacctgg
    37141 ctgctttcca gctttgtcgc ctggaaacaa acaacggata acgtcgatga attgttcgac
    37201 acccaactga tgctgtttgc caagcggtta agtacgctcg atctcaacga aatcaacgcg
    37261 gcggatcgca tggcacagac gccaaataaa ttaaaacacg gtcatgttga tgacgatgcg
    37321 ctgacctttg ccatctttac ccacgacggc agaatggtcc ttaatgatgg cgataacgga
    37381 gaagatattc cctatagcta tcaacgggaa ggttttgctg acgggcaact ggtcggtgaa
    37441 gacgatcctt ggcgttttgt ctggatgacc tcacctgatg gcaaatatcg catcgttgtt
    37501 ggccaggaat gggaataccg tgaagacatg gcgctggcga ttgttgccgg gcaattgatc
    37561 ccgtggctgg tcgcactgcc gattatgtta atcatcatga tggtactact gggtcgtgaa
    37621 ctcgcgccgc tgaacaaact ggcgctggca ctacgtatgc gtgaccctga ctcggaaaaa
    37681 ccactaaacg cgactggcgt acccagcgaa gtgcgaccac tggttgagtc gctaaatcaa
    37741 ctgttcgccc gcacacatgc gatgatggtt cgagaacgac gctttacctc cgatgcagct
    37801 cacgaactac gtagcccgtt aacagcgctg aaagtgcaaa ccgaagttgc gcagctctct
    37861 gacgatgatc cgcaggcgcg gaaaaaagca ctgctccaat tacattccgg gatcgatcgc
    37921 gctactcgtc tggttgatca actgctcacg ctatcgcggc tggactcact ggataacctt
    37981 caggacgtcg cggagatccc gcttgaagat ctcctgcaat cgtcggtgat ggatatttac
    38041 cacacggcgc agcaggcgaa aattgacgtg cgactgacac tcaatgccca cagcatcaaa
    38101 cgcactggac aaccgctatt gctaagtttg ttggtgcgaa accttctgga taacgccgtg
    38161 cgctacagtc cacagggcag cgtggtggac gtcacgctga atgccgataa tttcatcgtg
    38221 agggataacg gccccggtgt gacaccagag gcactggcgc gaattggcga acgcttctat
    38281 cgcccccccg gacaaaccgc taccggcagt ggacttgggc tatcgattgt ccagcgaatc
    38341 gccaaattgc atggcatgaa tgttgaattt gggaatgcgg aacaaggtgg atttgaggcg
    38401 aaggtgagct ggtaagggta agacttttgc taaattcgca aaagactttg cacattttgc
    38461 taattttacc gtaccgctct gtgacgtact atagtcgaca aacgtctcac cttgaggtta
    38521 aaaaatgagc aacaccctga ttatcaacgg cgcgaaaaaa ttcgcccact ccaatggtca
    38581 actgaacgac accctgaccg aagtcgcgga tggcacactg cgcgacctcg ggcatgatgt
    38641 tcgcatcgtt cgcgccgaca gcgactacga tgtcaaagcg gaagtacaaa actttctctg
    38701 ggctgatgtg gtgatctggc agatgccagg ctggtggatg ggcgcgccgt ggacagtgaa
    38761 aaaatacatt gatgatgtat tcaccgaagg tcacgggacg ctgtatgcca gcgatggccg
    38821 tacccgcaaa gatccgtcaa aaaaatacgg ttccggcggc ctggtacagg gcaaaaaata
    38881 tatgctttct ctgacctgga acgcaccaat ggaagccttc accgaaaaag atcaattctt
    38941 ccacggcgtt ggcgtcgacg gtgtgtatct gccgttccat aaagcaaacc aattccttgg
    39001 catggaaccg ctgccgacat ttatcgctaa tgacgtgata aaaatgcctg atgttccccg
    39061 ctatactgaa gaatatcgca agcatcttgt ggaaattttt ggttaactag agctcaggct
    39121 ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc cgtactcgtc ctggtcaaca
    39181 tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt ccaaccgtac tgaaagaaga
    39241 aggttgccac ggctatgcgc caatggtgga ttgcgctgct ggcgtgagtt tccagtctat
    39301 ggcaccggat tctatcgtga tgattgagca gtgggaaagc atcgcgcacc ttgaagcgca
    39361 tctgcaaacc ccgcacatga aggcgtacag cgaagccgta aaaggcgacg tgctggagat
    39421 gaatattcgt attctgcagc cagggattta atcctgcctt gtttgcccgg ccatcctgac
    39481 cgggcaatgt tctttccttt aaacctcaat ctccgccatg tcgcctttct cttgcaacca
    39541 gttgcggcga tcttccgagc gtttcttcgc cagcagcata tccatcatcg cgtcagtacg
    39601 ctgatcgtct tcatcatcga tagtcaactg caccagacgg cgagtgttcg gatcaagcgt
    39661 ggtttcacgc aattgcatcg ggttcatttc ccccagacct ttaaaacgct ggacgttcgg
    39721 cttgcctttc ttgcgtttta attgctcaag tacgccctct ttctcttctt ccgtcagcgc
    39781 gtaataaacc tctttcccga gatcaatacg gtagagcggt ggcagtgcga cgtaaacgtg
    39841 accgtgtttc accaacgcgc ggaagtgttt tacgaacaaa gcgcagagca gcgtggcaat
    39901 gtgcagacca tcagagtccg catccgcgag gatacagatt ttgccataac gaagctggct
    39961 cagatcgtcg ctgtcaggat cgataccgat cgctaccgaa atatcatgca cttcctgcga
    40021 ggccagcact tcgtcggaag aaacttccca ggtgttaagg atcttacctt tcagcggcat
    40081 gatcgcctga tattcacgat cgcgcgcctg cttggcagat ccgcctgcgg agtcaccttc
    40141 cacgaggaac agttcggtac ggttaaggtc ctgcgcggta caatcagcca gtttgccagg
    40201 cagcgccggg ccgctggtca gctttttacg caccactttt ttagccgcac gcatacgacg
    40261 ctgggcgctg gaaatcgcca tctctgccag caactccgcc gcctgaacgt tctggttcag
    40321 ccacaggata aaggcatctt tcaccacgcc agaaacgaat gccgcgcatt gacgcgaaga
    40381 gagacgctct ttcgtctgcc cggcaaactg cggatcctgc attttcaccg acagaacata
    40441 ggcgcagcga tcccagatat cttccgccga cagcttcacg ccgcgcggca gaatgttgcg
    40501 atattcacag aactcacgca tcgcgtccaa caggccctga cgcaggccat taacatgggt
    40561 accgccctgc atcgttggga tcaggttgac gtagctttcg gtcagcagtt caccgccttc
    40621 cggcagccac agtagcgccc agtccacagc ttcagtatcg ccagcgaaat taccgataaa
    40681 cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc gccaggtaat cattcagacc
    40741 gtcctgatag caccagcgct gttcggtgtt gttgatctca tctttaaagg taatctcaac
    40801 gccggggcac agtaccgctt tggctttcag cacatgcgtc aggcgtgaaa cagaaaatcg
    40861 cgggctgtca aagaaggttt catccggcca gaagtgcacg ctggtaccgg tattgcgttt
    40921 accgcaagtg ccgacaacct gtaaatcctg caccttttcg ccattttcaa aggcgatgtt
    40981 ataaacctga ccatcgcggc gcacgttaac ttcaacgcgc ttcgacaggg cgttaaccac
    41041 cgaaatcccc acgccatgca ggccgccaga gaactggtaa tttttgttag agaatttacc
    41101 gcctgcatgc agacggcaaa gaatcaattc aaccgccggg acaccctctt ccgggtgaat
    41161 atccaccggc atcccgcgcc catcgtcaat aacttctaac gactggtcag catgtaagat
    41221 tacatccacg cgttttgcgt gacccgccag tgcttcatcg acactgttat caataacttc
    41281 ttgccccaaa tggttagggc gagtggtatc ggtatacatc cccggacggc ggcgaaccgg
    41341 ctcaagcccg gtgagtacct caatggcatc agcgttataa gtttgcgtca tggtttaagt
    41401 tagcaattcg agttgatcgt cagagatggt gcagaccaag aaaatcgacg atcgggttga
    41461 aataatcttc gaagcccgtg aatgcgtggt tgccgccttc tatgacagtc tggcggcagg
    41521 aagcgtagta cgccaccgcc tggcggtaat ccagcacttc atctcccgtc tgttgcagca
    41581 gccagatcaa atccggcgct tccagcgggt caatctgcat gactttgaga tcgtaaatat
    41641 ggcgtgactc tagcacatat tgctgcccgg tgtatgggtt ctcgttctga ccgagatagt
    41701 ccgtcagcag ttcaaacggg cgcaccgccg ggtttaccac cactgcgggc agcataaaac
    41761 attgtgacaa ccaggtggcg taatatcccc ccagtgacga accgacaata cccagcgaat
    41821 cgccgccatg ttccaggaca atggattcca gcagttctgc cgcgtcggaa ggatacggcg
    41881 gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc cgccagccag ttttttaaca
    41941 agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg taaataaaga agcgtagaca
    42001 tcagtagcct tctgaagcgg tatcaggttg gaaacgtgtg tccgccaggc gatgcacctc
    42061 ggtggtcagc gtgccatcag catgtaactc gagagtacgc cagccgggcg cgatagtatc
    42121 cagcgtaaag ttggaacagt gcggcttaaa ctgcacacag gtcgacggcg ttgccagcag
    42181 gcggcgaccg ttccagtcga gatccagctc ctgatgaata tgaccgcaca gcaagtattt
    42241 gacgtgcgga aacttcgcca gcacggtatc cagttcgccc gcgttacgca gactgtgttg
    42301 atcgagccaa ctacaacccg caggtagcgg atgatgatgc agcagcagca acgtatggcg
    42361 ttctggcgca tcggccagtt tacgttccag ccactcaagc tgaaactcgc tcagctcacc
    42421 gtgcggcacg ccaaacacct ggctatccag caacaggatt tgccattgct caccaataaa
    42481 cacccgcttc gccggggaga tacccgcatc ctgtaacgcg ctgtacatcg cgggctggaa
    42541 atcgtggttg cccggcagcc agacgcaggg cgcacgaaaa cttgcgatgc cttcagcgaa
    42601 atgctgatag gccgcagagg attgatcctg cgctaaatca cctgtcgcga caatcaggtc
    42661 gaattcgtgc tgatgtggac gaatcgcctc cagcaccgcc tggtagcttt cccaggtgtt
    42721 tacccctaac agagcttcgt gcttttgtgc aaacaggtga gtgtcggtaa tttgtaaaat
    42781 cctgactctg gcctcaccag ccagaggaag ggttaacagg ctttccaaat ggtgtcctta
    42841 ggtttcacga tgctagtaaa ccggaatcgc catcgctcca tgtgctaaac agtatcgcaa
    42901 ccagtccgct aaaaactgat taatttgatg cttttcgtcg cgttgatgca actttttatt
    42961 aggataatca taccgcgctt tgaagcgaaa aatctgctgg cttgaacaca cttcagccac
    43021 catcgcgtca tgatacagac gcaccgtcat tgacggaagg ctccagtaac tgatcgcggg
    43081 cgcagtctgt tctattgtca ccagggtagt gtatcgggtc gattccacaa tcgtcagccg
    43141 atattgtgcg tttgccacct gatagcttac agtttcgccg ggtgcgtcat tgcgcggtaa
    43201 caaacggcgc aattgtgaaa agttcatctc gcacaggcgc atcatttcag gaaagtcagg
    43261 tgtgtaacgc ttcatttatg cccactcatt ttttaacgct tgatgatgca gctgcagcca
    43321 ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc tcttctaccc actggtatgc
    43381 ctgttcccgg cttaccacat gaacgcgaat atcttcgttt tcatcagcca gaccatgaat
    43441 accgcttgcg gtcgtggcgt ccacttcgcc caccataatt gacgaacgct cactggtgcc
    43501 ccccgggctt gccaggaaac ttaacaccgg tttggtccgt ttgactatca gtcccgcctc
    43561 ttcaatcgct tcgcgacggg caacatcttc cacactttca ccctcttcta tcatcccggc
    43621 aaccatctcc agtagccagg gggtttcact ggtgtcgtat gcggcaatcc gaatctgctc
    43681 aatcagcaca acttcatcgc gcactgggtc aaagggtagc aagactgcgg cgtgaccgcg
    43741 ctcaaaaatt tcccgccgta cctcatgact catttgcccg ttgaatagac gatgacgaaa
    43801 tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt tctcgtgcaa taatttctac
    43861 atcgtttttg ccaaatgtaa cgggcaggtt gtctggctta agcattgtta atgtcctggc
    43921 actaatagtg aattaaatgt gaatttcagc gacgtttgac tgccgtttga gcagtcatgt
    43981 gttaaattga ggcacattaa cgccctatgg cacgtaacgc caaccttttg cggtagcggc
    44041 ttctgctaga atccgcaata attttacagt ttgatcgcgc taaatactgc ttcaccacaa
    44101 ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc tgagcctttc tgggttcagt
    44161 tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc aagcacgcct tagtaacccg
    44221 gaattgcgta agtctgccgc cgatcgtgat gctgcctttg aaaaaattaa tgaagcgcgc
    44281 agtccattac tgccacagct aggtttaggt gcagattaca cctatagcaa cggctaccgc
    44341 gacgcgaacg gcatcaactc taacgcgacc agtgcgtccc tgcagttaac tcaatccatt
    44401 tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa aagcagcagg gattcaggac
    44461 gtcacatatc agaccgatca gcaaaccttg atcctcaaca ccgcgaccgc ttatttcaac
    44521 gtgttgaatg ctattgacgt tctttcctat acacaggcac aaaaagaagc gatctaccgt
    44581 caattagatc aaaccaccca acgttttaac gtgggcctgg tagcgatcac cgacgtgcag
    44641 aacgcccgcg cgcagtacga taccgtgctg gcgaacgaag tgaccgcacg taataacctt
    44701 gataacgcgg tagagcagct gcgccagatc accggtaact actatccgga actggcggcg
    44761 ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg ttaacgcgct gctgaaagaa
    44821 gccgaaaaac gcaacctgtc gctgttacag gcacgcttga gccaggacct ggcgcgcgag
    44881 caaattcgcc aggcgcagga tggtcactta ccgacgctgg atttaacggc ttctaccggg
    44941 atttctgaca cctcttatag cggttcgaaa acccgtggtg ccgctggtac ccagtatgac
    45001 gacagcaata tgggccagaa caaagtgggc ctgagcttct cgctgccgat ttatcagggc
    45061 ggaatggtta actcgcaggt gaaacaggcc cagtacaact ttgttggtgc cagcgagcaa
    45121 ctggaaagcg cgcatcgtag cgtcgtgcaa accgtacgtt cctccttcaa caacattaat
    45181 gcatctatca gtagcattaa cgcctacaaa caagccgtag tttccgctca aagctcatta
    45241 gacgcgatgg aagcgggcta ctcggtcggt acgcgtacca ttgttgatgt gttggatgca
    45301 accaccacgc tgtacaacgc taagcaagag ctggcaaatg cgcgttataa ctacctgatt
    45361 aaccagttga atattaagtc agctctgggt acgttgaacg agcaggatct gctggcactg
    45421 aacaatgcgc tgagcaaacc ggtttccact aatccggaaa acgttgcacc gcaaacgccg
    45481 gaacagaatg ctattgctga tggttatgcg cctgatagcc cggcaccagt cgttcagcaa
    45541 acatccgcac gcactaccac cagtaacggt cataaccctt tccgtaactg acgacgacga
    45601 cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga tacgggttat ctgccgcatt
    45661 cttccccctt ctcgcttcaa tttcgaccag ccatcctcta ttctgatggg tatttaccac
    45721 tggtcccgga agacaaaaat gaaacggaca aaatccatac gccacgcatc gttccgcaaa
    45781 aactggagcg cacgccatct gacaccagtc gctctcgcgg ttgccactgt ttttatgctg
    45841 gctggctgtg aaaagagtga tgaaacagtg tctctctatc aaaatgctga cgactgttca
    45901 gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt acaacaatgc gctgaaagaa
    45961 gccgaacgta ctgcgccgaa atacgccacc cgtgaagact gtgttgctga atttggtgaa
    46021 ggtcagtgcc agcaggcacc agcccaggct ggcatggcac cagaaaacca ggcgcaggcc
    46081 cagcaatcca gcgggagttt ctggatgccg ctgatggccg gttacatgat ggggcgtctg
    46141 atgggcggcg gcgcgggatt tgcacagcag ccgctgttct cctcgaaaaa cccagccagc
    46201 ccggcttacg gtaaatatac cgacgcgacg ggtaaaaact atggcgcagc ccagccaggc
    46261 cgcaccatga ccgtaccgaa gacagccatg gcaccaaaac cggcgaccac tactaccgtt
    46321 acccgtggcg gttttggtga atctgttgcc aaacaaagca ctatgcagcg tagcgcaacc
    46381 ggcacctctt ctcgttcaat gggtggctga taccgatgga aagagtcagt attaccgagc
    46441 gcccggactg gcgcgaaaaa gcccacgaat acggtttcaa ttttcacacc atgtacggcg
    46501 agccgtactg gtgtgaagat gcttactaca agttgaccct cgcccaggtt gaaaagctgg
    46561 aagaagtcac cgccgaactg caccagatgt gcctgaaagt ggtggaaaaa gtgatcgcca
    46621 gcgatgagct gatgaccaaa ttccgcattc caaaacgcac ctggagtttt gtgcgtcagt
    46681 catggctgac tcaccagcca tcgctttatt cgcgtcttga tctggcatgg gatggcactg
    46741 gcgaacctaa actgctggaa aataacgccg atacaccaac gtcactatac gaggcggcgt
    46801 tctttcagtg gatctggctg gaagatcagc ttaacgccgg taacttgccg gagggcagcg
    46861 accagtttaa cagtctgcaa gaaaagctaa tcgatcgctt tgtcgagttg cgtgaacagt
    46921 atggcttcca gttgctgcat ctcacctgct gtcgcgacac ggtggaagat cgcggaacca
    46981 ttcagtattt gcaggactgc gcgacggaag ctgaaatcgc taccgagttc ctctacattg
    47041 acgatatcgg gttaggtgaa aaaggtcagt tcacggattt acaggatcag gtaatttcca
    47101 acctgttcaa gctgtatcca tgggaattta tgttgcgtga gatgttctcc accaagctgg
    47161 aggatgcagg cgtacgctgg ctggaaccgg cgtggaagag cattatctcc aacaaagcgc
    47221 ttctaccgct actgtgggag atgttcccga atcacccgaa cctgctgccc gcttattttg
    47281 cggaagatga tcatccgcaa atggaaaaat atgtggttaa accgatcttc tcccgtgaag
    47341 gcgcaaacgt gtcgatcatt gagaacggca aaaccattga agcagcggaa ggtccgtatg
    47401 gcgaagaagg gatgattgtt cagcaattcc acccgttacc gaaattcggc gacagctata
    47461 tgctgattgg tagctggctg gtgaacgatc aacctgccgg aattggcatt cgtgaagacc
    47521 gtgcgttgat cacccaggat atgtctcggt tttatccaca tatttttatt gagtaatcaa
    47581 tgatgccgga tggcattcgc catccggtaa ttgttaacct atctgcaccg acagcatact
    47641 caggctgccc atttctatac catcgaccgg aatggtaatt ggctcctgcc catcccacgc
    47701 acctaagaca tacaacaacg gcagatagtg ctccggcgtt gggttcgata acgcgccacc
    47761 ttcatggtca aggtaattca ccagaggatg ctgctctacc gccccttgcc acgtcagatt
    47821 cgctttcaca tactcattaa acgacatcgc ccaaggatat ggtgaactat caccgtgcca
    47881 cttcactgta cgcaggttat gcaccacgtt gccactggcg accaacatta ttccttcatc
    47941 tcgcagcgct gccagtttgc gccccatttc gaaatgccag gcggcaggtt tgctactgtc
    48001 gatactcaac tgcaccatcg ggatatcggc gtcaggatac atcttaatca gcacgcccca
    48061 ggaaccgtgg tcaaagcccc aggcttcttt atccagcgtc accgggaccg acgctaacag
    48121 ctcaaccaga tgctgtgcca gcacaggcga acccggagca ggataatgcg tatcgtacag
    48181 cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc gtctccatcg cggtcactcc
    48241 tgttccacgg gtaaaccagt gagccgaaac caccacaatc gcttgcgggc gtggcaatgt
    48301 catccccaac ttctgccagc tgcgggtata caaattatct tccagcacgt tcatcggact
    48361 accgtgacct aaaaacaatg ctggcatacg tgttgaagac atgatgatat ccttaactaa
    48421 aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg gagtaagaac ccggataaca
    48481 atgatgatga tcatcagtta ttttgacgat ctgcctgaag gtgaagattt ataaggagtt
    48541 gtcgatgtca gtacctctca ttctgaccat actggcgggg gcagccacgt ttattggcgc
    48601 gtttctcggc gttctcgggc aaaaaccctc gaaccgctta ctggcgtttt cgctaggatt
    48661 tgcggcgggg atcatgttgc tcatctcatt aatggaaatg cttcctgccg cactagcagc
    48721 tgaaggaatg tcgcctgtgt tgggttatgg aatgtttatc ttcggtctgc ttggctattt
    48781 tggcctggac cgcatgttgc cacatgcgca tccgcaggat ttaatgcaaa aatcggtgca
    48841 gccgttgcca aaatcgatca agcgcacagc cattctgctc actctcggca tcagtctgca
    48901 taacttcccg gaagggattg ccacctttgt cacggcgagc agcaacctgg agctgggatt
    48961 tggcatcgca ctggccgtcg cgttgcacaa tatccctgaa ggtctggcag tggcaggccc
    49021 ggtttatgcg gcaacggggt ctaaacgcac cgcgattctg tgggcgggga tttctggact
    49081 agcagaaatt cttggtggtg tgctggcgtg gttaatcctc ggtagcatga tttccccggt
    49141 ggtcatggcg gcaatcatgg cggcggttgc aggaattatg gtggcgctct cggttgatga
    49201 attaatgccg ctcgccaaag agattgaccc taataataac cccagctatg gcgtactatg
    49261 tggaatgtca gtgatgggat tcagtttagt gctgctacaa acggcgggaa ttggttaaaa
    49321 acaacatcag aagttgtcca gcacatggac aacttctgcc agcttcacga aagccttatt
    49381 ctaccttttc ggctgtctct tctctcgtac tgtttaagta tttgttccgc gcttcaagca
    49441 gtgcgtctct gtcctggcga aacgcccgac aataacgttt caaatgcagg aaataaccca
    49501 ccacaaccag agcaatcaat acaatccagt accaggcgat aaacatcctt ttacccttaa
    49561 tatttaacaa tactaaacat ttagcgcata aatttcacat atcctttttc ggatatattc
    49621 atatggtcgc ataataacgg aacaattata tgattttgat cataatgaaa aagaatgcct
    49681 gatattaatg aaattttctc atagaaagag aagcggaatt aacatcttgc atagcagggc
    49741 aaaataaaaa ccgaagccag ctacaacttc ggtttcttga ttaaggcagt aattaagcag
    49801 cggttttcag ctggctttac gctcatgtgc ctgacggtat gccaccaggt cttcaatagt
    49861 cacgagcgcc atattgtgtt tattggcaaa ctcaatgcac tctggtgcac gcgccatcgt
    49921 gccatcgtca ttagtcagct cacacagcac accagccggt ttaaagcctg ccagcgtcat
    49981 caaatcaata gtcgcttcag tatgaccgcc acgcgtcagt acaccacctg cctgcgcgcg
    50041 aagtgggaaa acgtggcccg ggcgattcag atcagaaggt tttgcgccat cggcaatcgc
    50101 tgcgcgaacg gtcgtaatac gatcagcggc agaaacaccg gtagtcacac cttcagccgc
    50161 ttcaatggtc acggtaaaac cggtgccata ggcgctggtg ttattttcta ccatcattgg
    50221 cagatcaagt tgtttacggc gatcttcagt gatgcacagg caaacaatac cgctaccgtg
    50281 gcgaatggtc agcgccatct gctcaacggt catggtttct gccgggaaga tcatatcgcc
    50341 ttcgttttca cggtcttcat catcaagcac cattacaccg cgtccttcac gcagcgcagc
    50401 cagtgcattt tcaacacgtt cgaaaggcgt accaaaagag gaaagtagcg tctgattcat
    50461 ggtaaaaaaa cctcactaaa attatggtta ccagaatcag ggcagtctta ggagtggcgg
    50521 catatagcca aaataacgtg agcgggtcca tgcccgacag aatcgttact ctctcccatc
    50581 cggactctaa ccgtcggccc cggaattaca ccggatctgc tgtcctttga gttcgcaccc
    50641 aaagcgctcg cgggctttca actgagttga tttaccgccg gtggggaatt tcgccccgcc
    50701 ctgagaataa gcggattcac tataacgcta atgattagcg gcagcaacgc atagcttcac
    50761 ataattctgg tttatgactt acccttatcg cactacaatg gtactcaaca cctatcagta
    50821 cagggaaact acaatgattg acccgaaaaa aattgagcaa atcgctcgcc aagttcacga
    50881 atcaatgcct aaaggaatca gggagttcgg ggaagatgtg gagaaaaaaa tccgccaaac
    50941 cctacaagct cagctgacgc gcctcgacct tgtaagccgt gaagagttcg acgtccaaac
    51001 gcaagtcctg ttacggactc gtgagaaact ggcgcggctt gaacaaagaa tgagcgaact
    51061 ggaaaatcgc agtactgaga ttaaaaagca gccagatcca gagactctcc ccccaacgct
    51121 gtaaacattt gtctttaatg cactgtaaat tatgaattaa tcttctaatt tacagtgctt
    51181 tctattacct tatcgcttaa ttccattcag aatatcatca c
//
LOCUS       DAZPLI010000012        49280 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZPLI010000012 DAZPLI010000000
VERSION     DAZPLI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49280
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.012"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            261..1355
                     /gene="gcvT"
                     /locus_tag="ACHUU3_000656"
     CDS             261..1355
                     /gene="gcvT"
                     /locus_tag="ACHUU3_000656"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR2080822.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            1379..1768
                     /gene="gcvH"
                     /locus_tag="ACHUU3_000657"
     CDS             1379..1768
                     /gene="gcvH"
                     /locus_tag="ACHUU3_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR2080823.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1887..4760
                     /gene="gcvP"
                     /locus_tag="ACHUU3_000658"
     CDS             1887..4760
                     /gene="gcvP"
                     /locus_tag="ACHUU3_000658"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR2080824.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            5026..5769
                     /locus_tag="ACHUU3_000659"
     CDS             5026..5769
                     /locus_tag="ACHUU3_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR2080825.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5826..7259)
                     /gene="bglA"
                     /locus_tag="ACHUU3_000660"
     CDS             complement(5826..7259)
                     /gene="bglA"
                     /locus_tag="ACHUU3_000660"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR2080826.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            7304..7615
                     /gene="yqfB"
                     /locus_tag="ACHUU3_000661"
     CDS             7304..7615
                     /gene="yqfB"
                     /locus_tag="ACHUU3_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFR2080827.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            7779..8438
                     /locus_tag="ACHUU3_000662"
     CDS             7779..8438
                     /locus_tag="ACHUU3_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR2080828.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8634..9614)
                     /gene="ygfZ"
                     /locus_tag="ACHUU3_000663"
     CDS             complement(8634..9614)
                     /gene="ygfZ"
                     /locus_tag="ACHUU3_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR2080829.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9857..10123
                     /gene="sdhE"
                     /locus_tag="ACHUU3_000664"
     CDS             9857..10123
                     /gene="sdhE"
                     /locus_tag="ACHUU3_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR2080830.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10104..10511
                     /locus_tag="ACHUU3_000665"
     CDS             10104..10511
                     /locus_tag="ACHUU3_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR2080831.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10551..11072)
                     /gene="fldB"
                     /locus_tag="ACHUU3_000666"
     CDS             complement(10551..11072)
                     /gene="fldB"
                     /locus_tag="ACHUU3_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR2080832.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11184..12080
                     /gene="xerD"
                     /locus_tag="ACHUU3_000667"
     CDS             11184..12080
                     /gene="xerD"
                     /locus_tag="ACHUU3_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR2080833.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            12105..12815
                     /gene="dsbC"
                     /locus_tag="ACHUU3_000668"
     CDS             12105..12815
                     /gene="dsbC"
                     /locus_tag="ACHUU3_000668"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR2080834.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            12821..14554
                     /gene="recJ"
                     /locus_tag="ACHUU3_000669"
     CDS             12821..14554
                     /gene="recJ"
                     /locus_tag="ACHUU3_000669"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR2080835.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14645..15743
                     /gene="prfB"
                     /locus_tag="ACHUU3_000670"
     CDS             join(14645..14719,14721..15743)
                     /gene="prfB"
                     /locus_tag="ACHUU3_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR2080836.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15753..17270
                     /gene="lysS"
                     /locus_tag="ACHUU3_000671"
     CDS             15753..17270
                     /gene="lysS"
                     /locus_tag="ACHUU3_000671"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR2080837.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17313..17861)
                     /gene="idi"
                     /locus_tag="ACHUU3_000672"
     CDS             complement(17313..17861)
                     /gene="idi"
                     /locus_tag="ACHUU3_000672"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR2080838.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            17916..17987
                     /locus_tag="ACHUU3_000673"
     CDS             17916..17987
                     /locus_tag="ACHUU3_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2080839.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(17984..18109)
                     /gene="yqfG"
                     /locus_tag="ACHUU3_000674"
     CDS             complement(17984..18109)
                     /gene="yqfG"
                     /locus_tag="ACHUU3_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR2080840.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(18111..19179)
                     /locus_tag="ACHUU3_000675"
                     /pseudo
     CDS             complement(18111..19179)
                     /locus_tag="ACHUU3_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
     gene            19615..21534
                     /gene="ygfT"
                     /locus_tag="ACHUU3_000676"
     CDS             19615..21534
                     /gene="ygfT"
                     /locus_tag="ACHUU3_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFR2080841.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            21534..22022
                     /locus_tag="ACHUU3_000677"
     CDS             21534..22022
                     /locus_tag="ACHUU3_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR2080842.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(22091..22441)
                     /gene="ghxQ"
                     /locus_tag="ACHUU3_000678"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             complement(22091..22441)
                     /gene="ghxQ"
                     /locus_tag="ACHUU3_000678"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(22477..22791)
                     /locus_tag="ACHUU3_000679"
     CDS             complement(22477..22791)
                     /locus_tag="ACHUU3_000679"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR2080843.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            complement(22724..25663)
                     /locus_tag="ACHUU3_000680"
     CDS             complement(22724..25663)
                     /locus_tag="ACHUU3_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFR2080844.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            complement(25660..26439)
                     /gene="ygfM"
                     /locus_tag="ACHUU3_000681"
     CDS             complement(25660..26439)
                     /gene="ygfM"
                     /locus_tag="ACHUU3_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFR2080845.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(26490..27818)
                     /gene="ssnA"
                     /locus_tag="ACHUU3_000682"
     CDS             complement(26490..27818)
                     /gene="ssnA"
                     /locus_tag="ACHUU3_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFR2080846.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(27821..30919)
                     /gene="ygfK"
                     /locus_tag="ACHUU3_000683"
     CDS             complement(27821..30919)
                     /gene="ygfK"
                     /locus_tag="ACHUU3_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFR2080847.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSS
                     TPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(31241..31819)
                     /gene="mocA"
                     /locus_tag="ACHUU3_000684"
     CDS             complement(31241..31819)
                     /gene="mocA"
                     /locus_tag="ACHUU3_000684"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFR2080848.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            31922..32692
                     /gene="yqeC"
                     /locus_tag="ACHUU3_000685"
     CDS             31922..32692
                     /gene="yqeC"
                     /locus_tag="ACHUU3_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFR2080849.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            32740..34365
                     /gene="yqeB"
                     /locus_tag="ACHUU3_000686"
     CDS             32740..34365
                     /gene="yqeB"
                     /locus_tag="ACHUU3_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFR2080850.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVEWKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILRVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(34406..35338)
                     /gene="arcC"
                     /locus_tag="ACHUU3_000687"
     CDS             complement(34406..35338)
                     /gene="arcC"
                     /locus_tag="ACHUU3_000687"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR2080851.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(35386..36771)
                     /gene="hyuA"
                     /locus_tag="ACHUU3_000688"
     CDS             complement(35386..36771)
                     /gene="hyuA"
                     /locus_tag="ACHUU3_000688"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFR2080852.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(36824..38035)
                     /locus_tag="ACHUU3_000689"
     CDS             complement(36824..38035)
                     /locus_tag="ACHUU3_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFR2080853.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(38093..39289)
                     /gene="dpaL"
                     /locus_tag="ACHUU3_000690"
     CDS             complement(38093..39289)
                     /gene="dpaL"
                     /locus_tag="ACHUU3_000690"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFR2080854.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(39347..40534)
                     /gene="ygeW"
                     /locus_tag="ACHUU3_000691"
     CDS             complement(39347..40534)
                     /gene="ygeW"
                     /locus_tag="ACHUU3_000691"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFR2080855.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            41013..42791
                     /locus_tag="ACHUU3_000692"
     CDS             41013..42791
                     /locus_tag="ACHUU3_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFR2080856.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTSASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(42831..43310)
                     /gene="xdhC"
                     /locus_tag="ACHUU3_000693"
     CDS             complement(42831..43310)
                     /gene="xdhC"
                     /locus_tag="ACHUU3_000693"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR2080857.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(43307..44185)
                     /gene="xdhB"
                     /locus_tag="ACHUU3_000694"
     CDS             complement(43307..44185)
                     /gene="xdhB"
                     /locus_tag="ACHUU3_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR2080858.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(44196..46493)
                     /gene="xdhA"
                     /locus_tag="ACHUU3_000695"
     CDS             complement(44196..46493)
                     /gene="xdhA"
                     /locus_tag="ACHUU3_000695"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFR2080859.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            46908..47663
                     /gene="actS"
                     /locus_tag="ACHUU3_000696"
     CDS             46908..47663
                     /gene="actS"
                     /locus_tag="ACHUU3_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR2080860.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            47742..47811
                     /locus_tag="ACHUU3_000697"
     tRNA            47742..47811
                     /locus_tag="ACHUU3_000697"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47774..47776,aa:Gly,seq:ccc)
     gene            48016..>49280
                     /locus_tag="ACHUU3_000698"
     CDS             48016..>49280
                     /locus_tag="ACHUU3_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFR2080861.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTLD
                     QIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAK"
BASE COUNT        11958 a        12167 c        13003 g        12152 t
ORIGIN      
        1 aattagcttt ctaatggcat catctttata tagaaactgg tgtgtttttt ggttttttat
       61 tctgtcgcga ttttggcgtt ttttaaccat aagctaatgt gatgatcaat tttaccttat
      121 ggttaacagt ctgtttcggt ggtaagttca ggcaaaagag aacgattgcg ttggggaccg
      181 ggagtggctc cgatgctggg tttcgtggtg ataatttcac catgaaaagt tgtcagcccc
      241 gcttattcaa tgaggacaag atggcacaac agactccttt gtacgaacaa cacacgcttt
      301 gcggcgctcg catggtggat ttccacggct ggatgatgcc gctgcattac ggttcgcaaa
      361 tcgacgaaca tcatgcggta cgtaccgatg ccggaatgtt tgatgtgtca catatgacca
      421 tcgtcgatct ccgcggcagc cgcacccggg agtttctgcg ttatctgctg gcgaacgatg
      481 tggcgaagct caccaaaagc ggcaaagccc tttactcggg gatgttgaat gcctctggcg
      541 gtgtgataga tgacctcatc gtctactact ttactgaaga tttcttccgc ctcgttgtta
      601 actccgccac ccgcgaaaaa gacctctcct ggattaccca acacgctgaa cctttcggca
      661 tcgaaattac cgttcgtgat gacctttcca tgattgccgt gcaagggccg aatgcgcagg
      721 caaaagctgc cacactgttt aatgacgccc agcgtcaggc ggtggaaggg atgaaaccgt
      781 tctttggcgt gcaggcgggc gatctgttta ttgccaccac tggttatacc ggtgaagcgg
      841 gctatgaaat tgcgctgccc aatgaaaaag cggccgattt ctggcgtgcg ctggtggaag
      901 cgggtgttaa gccatgtggc ttgggcgcgc gtgacacgct gcgtctggaa gcgggcatga
      961 atctttatgg tcaggagatg gacgaaacta tttctccttt agccgccaac atgggctgga
     1021 ctatcgcctg ggaaccggca gatcgtgact ttatcggtcg tgaagccctg gaagtgcagc
     1081 gtgagcatgg tacagaaaaa ctggttggtc tggtgatgac cgaaaaaggc gtgctgcgta
     1141 atgaactgcc ggtacgcttt accgatgcgc agggcaacca gcatgaaggc attatcacca
     1201 gcggtacttt ctccccgacg ctgggttaca gcattgcgct ggcgcgcgtg ctggaaggta
     1261 ttggcgaaac ggcgattgtg caaattcgca accgtgaaat gtcggttaaa gtgacaaaac
     1321 ctgtttttgt gcgtaacggc aaagccgtcg cgtgatttac ttttttggag attgattgat
     1381 gagcaacgta ccagcagaac tgaaatacag caaagaacac gaatggctgc gtaaagaagc
     1441 cgacggcact tacaccgttg gtattaccga acatgctcag gagctgttag gcgatatggt
     1501 gtttgttgac ctgccggaag tgggcgcaac ggttagcgcg ggcgatgact gcgcggttgc
     1561 cgaatcagta aaagcggcgt cagacattta tgcgccagta agcggtgaaa tcgtggcggt
     1621 aaacgacgca ctgagcgatt ccccggaact ggtgaacagc gaaccgtatg caggtggctg
     1681 gatctttaaa atcaaagcca gcgatgaaag cgaactggaa tcactgctgg atgcgaccgc
     1741 atacgaagca ttgttagaag acgagtaacg gctttattcc tcttctgcgg gagaggatca
     1801 gggtgaggaa aatttatgcc tcaccctcac tctcttcgta aggagagagg ttcacaattc
     1861 actgcacgtt tcaggaacca tcgctcatga cacagacgtt aagccagctt gaaaacagcg
     1921 gcgcttttat tgaacgccat atcggaccgg acgccgcgca acagcaagaa atgctgaatg
     1981 ccgttggcgc acaatcgtta aacgcgctga ccggccagat tgtaccgaaa gatattcagc
     2041 ttgcgacccc gccgcaggtt ggcgcaccgg cgaccgaata cgccgcactg gcagaactca
     2101 aggctattgc cagtcgcaat aaacgcttca cgtcttacat cggcatgggt tacaccgccg
     2161 tgcagctacc gccggttatc ctgcgtaaca tgctggaaaa tccgggctgg tataccgcgt
     2221 acactccgta tcaacctgaa gtctcccagg gccgccttga agcactgctc aacttccagc
     2281 aggtaacgct ggatttgact ggactggata tggcctctgc ttctcttctg gacgaggcca
     2341 ccgctgccgc cgaagcaatg gcgatggcga aacgcgtcag caaactgaaa aatgccaacc
     2401 gcttcttcgt ggcttccgat gtgcatccgc aaacgctgga tgtggtccgt actcgtgccg
     2461 aaacctttgg ttttgaagtg attgtcgatg acgcgcaaaa agtgctcgac catcaggacg
     2521 tcttcggcgt gctgttacag caggtaggca ctaccggtga aattcacgac tacactgcgc
     2581 ttattagcga actgaaatca cgcaaaattg tggtcagcgt tgccgccgat attatggcgc
     2641 tggtgctgtt aactgcgccg ggtaaacagg gcgcggatat tgtttttggt tcggcgcaac
     2701 gcttcggcgt gccgatgggc tacggtggcc cacacgcggc attctttgcg gcgaaagatg
     2761 aatacaaacg ctcaatgccg ggccgtatta tcggtgtatc gaaagatgca gctggcaata
     2821 ccgcgctgcg catggcgatg cagactcgcg agcaacatat tcgtcgtgag aaagcgaact
     2881 ccaacatttg tacttcccag gtactgctgg caaacatcgc cagcctgtat gccgtttatc
     2941 acggcccggt tggcctgaaa cgtatcgcta accgcattca ccgtctgacc gatattctgg
     3001 cggcgggcct gcaacaaaaa ggtctgaaac tgcgccatgc gcactatttc gacaccttgt
     3061 gtgtggaagt ggccgacaaa gcgggcgtac tgacgcgtgc cgaagcggct gaaatcaacc
     3121 tgcgtagcga tatcctgaac gccgtgggga tcacccttga tgaaaccacc acgcgtgaaa
     3181 acgtaatgca gcttttcagc gtgctgctgg gcgataacca cgggctggag atcgacacgc
     3241 tggacaaaga cgtggctcac gacagccgct ctatccagcc tgcgatgctg cgcgacgacg
     3301 aaatcctcac ccatccggtg tttaatcgct accacagcga aaccgaaatg atgcgctata
     3361 tgcactcgct ggagcgtaaa gatctggcgc tgaatcaggc gatgatcccg ctgggttcct
     3421 gcaccatgaa actgaacgcc gccgccgaga tgatcccaat cacctggccg gaatttgccg
     3481 aactgcaccc gttttgcccg ccggaacagg ctgaaggtta tcagcagatg attgcgcagc
     3541 tggctgactg gctggtgaaa ctgaccggtt acgacgccgt ttgtatgcag ccgaactccg
     3601 gcgcacaggg cgaatacgcg ggcctgctgg cgattcgtca ttatcatgaa agccgcaacg
     3661 aagggcatcg cgatatctgc ctgatcccgg cttctgcgca cggaactaac cccgcttctg
     3721 cacatatggc aggaatgcag gtggtggttg tggcgtgtga taaaaacggc aacatcgatc
     3781 tgactgatct gcgcgcgaaa gcggaacagg cgggcgataa cctctcctgt atcatggtga
     3841 cttatccttc tacccacggc gtgtatgaag aaacgatccg tgaagtgtgt gaagtcgtgc
     3901 atcagttcgg cggtcaggtt taccttgatg gcgcgaacat gaacgcccag gttggcatca
     3961 cctcgccggg ctttattggc gcggacgttt cgcacctcaa cctgcataaa actttctgca
     4021 ttccgcacgg cggtggtggt ccgggtatgg gaccgatcgg tgtgaaagcg catctggcac
     4081 cgtttgtacc gggtcatagc gtggtgcaaa tcgaaggcat gttaacccgt cagggcgcag
     4141 tttctgcggc accgttcggt agcgcctcta tcctgccaat cagctggatg tacatccgca
     4201 tgatgggcgc agaagggctg aaaaaagcaa gccaggtggc aatcctcaac gccaactata
     4261 ttgccagccg cctgcaggat gccttcccgg tgctgtatac cggtcgcgac ggtcgcgtgg
     4321 cgcacgaatg tattctcgat attcgcccgc tgaaagaaga aaccggcatc agcgagctgg
     4381 atattgccaa gcgcctgatc gactacggtt tccacgcgcc gacgatgtcg ttcccggtgg
     4441 cgggtacgct gatggttgaa ccgactgaat ctgaaagcaa agtggaactg gatcgcttta
     4501 tcgacgcgat gctggctatc cgcgcagaaa ttgaccaggt gaaagccgct gtctggccgc
     4561 tggaagataa cccgctggtg aacgcgccgc acattcagag cgaactggtc gccgagtggg
     4621 cgcatccgta cagccgtgaa gttgcggtat tcccggcagg cgtggcagac aaatactggc
     4681 cgacggtgaa acgtctggat gatgtttacg gcgaccgtaa cctgttctgc tcctgcgtac
     4741 cgattagcga ataccagtaa ttcactgatt cgactatttt ctaaaggcgc ttcggcgcct
     4801 ttttagtcag atgacaaagt acaaaagtgc tcagacagtc ccctcgcccc tttggggaga
     4861 gggttagggt gaggggaaca ggccggcact ggcgcgaata tttaccctca ccccggccct
     4921 ctccctgaaa gggcgagggg aaaagcgtgc caacattgaa gattgagcca gtttgttagc
     4981 aatctcaaag atacgtcaac gaattaattt ttctcggaaa aacaaatggc tatagcactt
     5041 gtgaccgatg gcagtcgcgg catcgggcgg gcaactgcat tactgttggc gaaagaagag
     5101 tatacggtgg cggttaatta tcaacaaaat ctccatgcgg cgcaggaagt ggtgaactta
     5161 atcacgcaag ccggtggcaa ggcattcgtg ctccaggcgg atatcagcga cgaaaatcag
     5221 gtcatcgcaa tgtttacagc aatcgatcag cacgatgaac cgctagcagc gctggtcaat
     5281 aacgccggga tcttgtttac ccagtgcacc gttgaaaacc ttaccgcaga gcgaatcaac
     5341 cgagtacttt ccaccaacat gacgggatat tttctctgct gccgcgaggc ggtaaaacgc
     5401 atggcgctta aaaatggtgg cagtggcggc gctatcgtca atgtctcttc ggtggcctca
     5461 cggttgggtt cgccagggga atatgttgat tacgcggcat cgaaaggggc gattgatacg
     5521 ttaaccaccg gactatcgct ggaagtcgcc gcgcaggggg tccgcgttaa ctgcgtgcgg
     5581 ccagggttta tttttaccga aatgcacgcc agcggcggcg agcctggacg cgtcgatcgc
     5641 gttaagtcga acatccccat gcagcgtggt ggacaggcag aagaggtcgc gcaggccatt
     5701 gtctggctac taagtgataa agcctcttac gtcacgggaa gttttatcga tttggcgggc
     5761 gggaaataaa acagggaagt tgtctgaccg gatgcaacaa gtattgcatc cggtacttca
     5821 tcgacttaaa gcttctcgcc gttgctggta atcacctctt tgtaccagtt aaagctcttc
     5881 ttacgtgaac gcgacatatc gccagtaccg tcgtcatgtt tattcacata gataaagccg
     5941 tagcgtttgc tgtactgccc ggtggtgaac gatacgcagt cgatgcagcc ccacggtgtg
     6001 tagcccatca gatccacgcc atcgtaagtc accgcttttt tcatctcttc gatatgggcg
     6061 cgcaggtagt caatgcggta gtcgtcgttg atgctgccat cttcttccac tttgtcgtaa
     6121 gcgccaaaac cgttttcgac aataaacagc ggcctctgat aacgctcata cagttcgcaa
     6181 agggcatagc gcagacctac tggatcaatc tgccagcccc agtcagacgc tttaacatac
     6241 gggttcggta cgctgccttc gaaaccagag atcgcatcgc cggtgccgcc ttcggccttc
     6301 actgcgttgg tcatgtaata gctgaaacca agataatcgc aggtgccttc gcgcagtaca
     6361 tcgagatcgc cgtcttccat tttgatgtta aatccgcgac gctcccactc gttcaacaca
     6421 taggacgggt aatagccgcg tagctgcaca tcggtaaaga cgtagcgttc gcgcatcgac
     6481 tcctgagcga acatcacatc gtccgggtta cagcagtagg gatagagcgg caccatcgcc
     6541 agcatacagc cgactttcat ctccgggtta atgcgacgcg cagctttcac cgccagggcg
     6601 ctggcgacaa actggtgatg cagcacctga tacatcgtct cttccgggtt ctcatgctcg
     6661 gtataaacca cgccggagca gcagtaaccg aacagcggtg cacgccagtt acgctggttg
     6721 ttaatttcgt tgaaggtcat ccagtatttg actttatgct tatagcgttc aaacaccact
     6781 tccgcgaaac ggacaaagaa atcaaccact ttacggttgg tccagctacc gtattgctgc
     6841 accagatgca gcggcatttc aaagtgggag agggtgatca ccggttcgat gttgtatttc
     6901 agcagttcat cgaacatatc atcgtagaac ttcagccctt cttcgtttgg ctgagcttca
     6961 tcgccttttg gaaaaatgcg cgtccaggct atggaagtcc gaaaacattt gaagcccatt
     7021 tcggcaaata gcttgatgtc ttccttgtag tgaccataaa aatcaacggc ttcatggttt
     7081 ggatagtatt tgcctggcaa gacttctttg gtgatttcgc gcggcacgcc gtgtgcgcca
     7141 ccggtcagaa cgtcacaaat gctcggccct ttgccgcctt tgttccagcc gccttcgacc
     7201 tgatgagcgg caactgcgcc gccccataag aaatctttct gtaaggtgag ttttttcact
     7261 atcataggct ccagatgagt caattcttac aggaaatagt accatgcagc caaacgacat
     7321 cacttttttt caacgtttcc aggatgacat tctggctggg cgtaaaacca tcaccatccg
     7381 cgacgagtct gaatcgcact tcaaaacggg tgatgtgctt cgtgtcgggc gttttgaaga
     7441 tgacggttat ttttgcacga ttgaagtcac cgcaacctca accgtaacgc tggatacgct
     7501 gacggaaaaa catgcagagc aggaaaatat gaccctgact gaactgataa aggtcattgc
     7561 cgacatctat cccggtcaga cacagtttta tgtgattgaa tttaaatgtc tttaaattga
     7621 actcacgcct gttagttgaa atttcgcatt taatttcatg atttttagga gattagtctc
     7681 aaatcagaat ttattttagc taacaggtgt tcactggaac tattctcagt tacgctggag
     7741 aggtatacat gtgcgtgtac gtttctccgg agtaagttat ggttcagaag cccctcatta
     7801 agcagggata ttcgctggca gaggaaattg ccaacagcgt cagtcacggc attgggttgg
     7861 tgtttggtat cgttgggctg gtgttgctac tggttcaggc ggtggatctc aatgccagcg
     7921 ccacagcgat aaccagttac agcctctatg gcggcagtat gatcctgctg ttcctcgctt
     7981 cgacgctcta tcacgccatt cctcatcaac gggcaaaaat gtggctgaag aaatttgacc
     8041 attgcgctat ttacctgttg attgccggaa cctacacgcc gtttttgctg gtggggctgg
     8101 attctccgtt agcgcgcggg ttgatgattg ttatctggag cctggcattg ctgggtattc
     8161 tgtttaaact gaccatcgcg caccgattca aaattttatc tctggtgacc tatctggcga
     8221 tgggctggct gtcgctggtg gtaatttatg aaatggcagt taagctcgcg gcgggcagcg
     8281 ttaccttatt ggcggtaggc ggcgtggttt attcgctcgg agtgattttc tatgtctgca
     8341 aacgcattcc ctacaatcat gccatctggc acggcttcgt gctcggcggc agtgtgtgcc
     8401 actttctggc gatctatttg tatattgggc aggcgtaatg ggtatgcctt tgtaggcctg
     8461 aaaagacgcg tcagcgtcgc atcaggcatc ggagcacaac tgccggattc ggcgtgaacg
     8521 ccttatccga tctacgggcc tcgtccctgt tgtgataaag gcatcaaatc ccagacatga
     8581 taagacgcgt cagcgtcgca tcaggcgata aatgcctgat gcgataaaga gatttactct
     8641 tcgagcgaat acggcagcgg ctcaatatgc aatgtattcg catcgtcgcg tacgcggaag
     8701 atgctatccg gttccatatc gttattcatg acgacctgta ccacgacctg accatcttcc
     8761 agttttaccg cagccagcac cgtaccggta cgacgccagt tctcgcccat tttcagctct
     8821 aagtcttcac cagcttccgg cagtcggctg gcgctacctg ccagcaacca gagcgcacgt
     8881 ttattggcac cacggaattt tgctcgcgcc accatctctt gtccggtata acagcctttc
     8941 ttaaagctga taccgcccag cgcctggaga ttggtcgcct gtgggataaa ctgcccgctg
     9001 ttggcggcat caatcaccgg gaaacccgct tcaatgttta atgccagcca ctgttggcta
     9061 ttgttcagtt ccgcttcacc gcgcagttta tcggtcagca tattagcagt agcttcatcg
     9121 gttacgatca ggaaacgttc tgccgggtat tcaaaccata gcaaagtggt cgcgccttct
     9181 ttaactacct gtttttcttt cgaaggcagt tcactaaaga tatttgccag cgcggcgcgc
     9241 gcctgaaaac cggcaacacc aagcagcaca tgctcgtcgt ctggcgcgat ggtcacttta
     9301 gagaacaccg catatttttt cagttcagtc agctgcggtt cacgcacgct gcgccgttca
     9361 atccacgcaa agccatcgcc gtcgcggaac agacgtaaat tgctccacat tttgcctttg
     9421 gcgtcgcaat gggcggcgag caggtgctga tcttctgcca tctggctgac atctgctgtc
     9481 acctgaccct gcatatattt ttcgctgtcc gcgccagtaa tggtggcaag cgcccagtca
     9541 tcaagcgtca tcagcgtcag cggtaaacgg gcagaagccg taggctgacg gggaggaaaa
     9601 ggtgtaaaag ccataataga gtcctgagtt gcttaacggc gtattaatga ctaatggtaa
     9661 aagagctatg aggcaatgca agtgctttca ctacgcgatc tgtgccttct gcgaggcgac
     9721 ttccaaaaaa atgaagccat tgaattgttg tgaattaatt tcgtgatcgc gatggtgttt
     9781 actgatattc ccgtaaaaga aggcgaaaaa cacgttacaa tagcggggta ctggattttc
     9841 gtgagacagg aagaagatgg acattaacaa caaagcccgc attcattggg catgccgccg
     9901 tggtatgcgc gaactcgata tttcaatcat gccgtttttc gaacatgagt acgacagctt
     9961 aagcgatgac gaaaaacgca tctttattcg tctgctggaa tgtgacgatc cggacctgtt
    10021 taactggctg atgaatcacg gtaaaccggc cgatgcagaa ctggaaatga tggtccgact
    10081 catccagaca cggaaccggg aacgtggtcc tgtggcaatc tgatttgcgc gtctcctggc
    10141 gcgcacagtg gctttccttg ctgattcatg ggctggttgc cgctgttatt ttactcatgc
    10201 cctggccact cagttacacc ccgttatgga tggtgttact ttcgctggtg gtgtttgatt
    10261 gcgttcgcag ccagcggcgt attaatgctc gccaggggga aattcgcttg ttgatggacg
    10321 ggcgtttgcg ttggcaaggg caggagtgga gcatcgtcaa agcaccgtgg atgattaaga
    10381 gcggcatgat gctgcgttta cgttctgatg gcggtaaacg gcaacattta tggctggcag
    10441 ccgacagcat ggacgaagcc gaatggcggg atttacggcg gattttgttg caacaagaga
    10501 cgcaaagata aagatgacgg caaagcatat gcgctaagcc gtcagtgaga tcaggcgtaa
    10561 tgctctgcca tttcgttgag gatttgctcg caccagctct gaatacgctc gtcgctaagg
    10621 tcatactggt tagtttcatc cagcgccaga cccacgaaca gttgcccgtc agcaatcacc
    10681 ggtttcgggc tggtaaattc atatccttcc gttggccagt agccgacgaa cttcacgcct
    10741 ttggtcgaga gtttgtcatg cagcataccg agcgcatcga ggaaccactc gccgtatccc
    10801 agttgatcgc caagcccata cagcgcaaca attttacctt caaggttcag gtcgtcgagc
    10861 tgatcccaga cggcttccca gtcttcctgg atttcaccaa aatcccaggt cgggataccc
    10921 agaatgagca catcgtactg ctccattaat ttcggggagt cgtccttgag gttatgtaag
    10981 gtcaccagtt ctgggccgat aatatcgcgg attttttctg ccgccatttc ggtgtaacag
    11041 gtgctggaac cgtaaaaaag acccatattc ataacgtaag cttctcgatt ctgctgtggc
    11101 ttttgcagct agtgtatcag aatcgcttta gtcacggcac aataaggcat aatggatcaa
    11161 atgagtgacc ataaggggcg caagtgaaac aggatctggc acgcatcgag cagtttcttg
    11221 atgctctgtg gctggaaaaa aatctggctg aaaatacgtt gaacgcttac cgtcgcgatc
    11281 tgtcaatgat ggtggagtgg ttgcatcacc gcgggttgac gctggcgacg gcgcaaagtg
    11341 acgatttgca ggcattactg gcagaacggc tggagggcgg gtataaagcc accagctcag
    11401 cgcgtttgct gagtgcagtg cgccgattgt tccagtatct ttatcgcgaa aagtttcgtg
    11461 aagacgatcc cagtgcgcat ctcgcttcac cgaaattgcc ccagcgtttg ccaaaagatt
    11521 taagcgaagc gcaggtcgaa cgtttattac aggcgccatt aattgatcag ccactggagc
    11581 tacgcgataa agccatgctt gaagtgttgt atgctaccgg gctgcgtgtc tctgaactgg
    11641 tcgggctgac aatgagtgat atcagcctgc gtcagggcgt agtgcgggtc attggtaaag
    11701 gcaacaaaga gcgcctggta ccgttaggtg aagaggcggt ttactggctg gaaacctatc
    11761 tggaacatgg gcgtccgtgg ctgctgaatg gtgtgtcaat tgacgtgttg tttcccagcc
    11821 agcgtgcgca gcagatgacg cgacagacct tctggcaccg cattaaacat tatgccgtgc
    11881 tggcgggtat cgacagcgaa aagctgtcac cgcatgtgtt gcgtcacgct tttgccactc
    11941 atttattaaa tcatggtgcg gatttacgcg tggtgcagat gctactgggc cacagcgatc
    12001 tttccaccac gcaaatttat acgcatgtcg ctaccgagcg tctgcggcaa cttcatcaac
    12061 agcatcaccc gcgggcgtga tgtctgaaaa gaacgggaag atttatgaag aaaggtttta
    12121 tgttgtttac tttgttagcg gcgttttcag gttttgctca ggctgatgac gcggcaattc
    12181 aacaaacgtt agccaaaatg ggcatcaaaa gcagcgatat tcagcccgcg cccgtagctg
    12241 gcatgaagac agttctgact aacagcggcg tgttgtacat caccgatgat ggtaaacata
    12301 tcattcaggg gccaatgtat gacgttagcg gcacggctcc ggtcaatgtc accaataaga
    12361 tgctgttaaa gcagttgaat gcgcttgaaa aagagatgat cgtttataaa gcgctgcagg
    12421 aaaaacacgt catcaccgtg tttactgata ttacctgtgg ttactgccac aaactgcatg
    12481 agcaaatggc agactataac gcgctgggga tcaccgtgcg ttatcttgct ttcccgcgcc
    12541 aggggctgga cagcgatgca gagaaagaaa tgaaagctat ctggtgtgcg aaagataaaa
    12601 acaaagcgtt tgatgatgtg atggcaggta aaagcgtcgc accagccagc tgcgacgtgg
    12661 atattgccga ccattacgca cttggcgtcc agcttggcgt tagcggtact ccggcagttg
    12721 tgctgagcaa tggcacactt gttccgggtt accagccgcc gaaagagatg aaagaattcc
    12781 tcgacgaaca ccaaaaaatg accagcggta aataattcgc gtgaaacaac agatacaact
    12841 tcgtcgccgt gcagtcgatg aaacggcaga cttgcccgct gaattgcctc ccttgctgcg
    12901 ccgtttatac gccagccggg gcgtgcgcag tgcgcaagaa ctggaacgca gtgttaaagg
    12961 gatgctgccc tggcagcaac tgagcggcgt cgaaaaggcc gttgagatcc tttacaacgc
    13021 ctttcgcgaa ggaacgcgga ttattgtggt cggtgatttc gacgccgacg gtgccaccag
    13081 cacggctcta agcgtgctgg cgatgcgctc gcttggttgc agcaatatcg actacctggt
    13141 tccaaaccgt ttcgaagacg gttacggctt aagcccggaa gtggtcgatc aggcccatgc
    13201 ccgtggtgcg cagttaattg tcacggtgga taacggtatt tcctcccatg cgggggttga
    13261 acacgctcgc tcgttgggca ttccggttat tgttaccgat caccatttgc cgggcgaaac
    13321 attacccgcg gcggaagcga tcattaaccc taacttgcgc gactgtaatt tcccgtcgaa
    13381 atcactggca ggcgtgggtg tggcgtttta tctgatgctg gcgctgcgca cctttttgcg
    13441 cgatcagggc tggtttgatg agcgcggcat cgcaattcct aacctggcag aactgctgga
    13501 tctggtagcg ctggggacag tggcggacgt cgtaccgctg gacgctaata atcgcattct
    13561 gacctggcag gggatgagtc gcatccgtgc cggaaagtgc cgtccgggga ttaaagcgct
    13621 gcttgaagtg gcaaaccgtg atgcacaaaa actcgccgcc agcgatttag gttttgcgct
    13681 ggggccacgt ctcaatgctg ccggacgact ggacgatatg tccgtcggtg tggcgctctt
    13741 gctgtgcgac aacatcggcg aagcgcgcgt gctggcaaat gaactcgatg cgctaaacca
    13801 gacgcgaaaa gagatcgaac aaggaatgca ggttgaagcc ctgaccctgt gcgagaaact
    13861 ggagcgcagc cgtgacacgc tacccggcgg gctggcaatg tatcaccccg aatggcatca
    13921 gggggttgtc ggtattctgg cttcccgtat taaagagcgt tttcaccgtc cggttatcgc
    13981 ctttgcgccc gcgggtgatg gtacgctgaa aggttctggt cgctccattc aggggctgca
    14041 tatgcgtgat gcgctggagc gattagacac actctaccct ggcatgatgc tgaagtttgg
    14101 cggtcatgcg atggcggcgg gtttgtcgct ggaagcggat aaattcgaac tctttcaaca
    14161 acggtttggc gaactggtta ctgagtggct ggacccttcg ctattgcaag gcgaagtggt
    14221 gtcagacggt ccgttaagcc cggccgaaat gaccatggaa gtggcgcagc tgctgcgcga
    14281 tgctggcccg tgggggcaga tgttcccgga gccgctgttt gacggtcatt tccggctgct
    14341 gcaacagcgg ctggtgggcg aacgtcattt gaaagtcatg gtcgaaccgg tcggcggcgg
    14401 tccgctgctg gatggtattg cttttaatgt cgataccgcc ctctggccgg ataacggcgt
    14461 gcgcgaagtg caactggctt acaagctcga tatcaacgag tttcgcggca accgcagcct
    14521 gcaaattatc atcgacaata tctggccaat ttagcgtcat cttctctata aaaaagagcg
    14581 tggattgggt acaatcccgc tcttatcacc gcattttgac tagctcaata aaagaaatca
    14641 gaccatgttt gaaattaatc cggtaaataa tcgcattcag gacctcacgg aacgctccga
    14701 cgttcttagg gggtatcttt gactacgacg ccaagaaaga gcgtctggaa gaagtaaacg
    14761 ccgagctgga acagccggat gtctggaatg aacccgaacg cgcacaggcg ctgggtaaag
    14821 agcgttcctc cctcgaagcc gttgtcgaca ccctcgacca aatgaaacag gggctggaag
    14881 atgtttctgg tctgctggaa ctggctgtag aagctgacga cgaagaaacc tttaacgaag
    14941 ccgttgctga actcgacgcc ctggaagaaa aactggcgca gcttgagttc cgccgtatgt
    15001 tctctggcga atatgacagc gccgactgct acctcgatat tcaggcgggg tctggcggta
    15061 cggaagcaca ggactgggcg agcatgcttg agcgtatgta tctgcgctgg gcagaatcgc
    15121 gtggtttcaa aactgaaatc atcgaagagt cggaaggtga agtggcgggt attaaatccg
    15181 tgacgatcaa aatctccggc gattacgctt acggctggct gcgtacagaa actggcgttc
    15241 accgcctggt gcgtaagagc ccgtttgact ccggcggtcg tcgccacacg tcgttcagct
    15301 ctgcgtttgt ttatccggaa gttgatgatg atattgatat cgaaatcaac ccggcggatc
    15361 tgcgcattga cgtttatcgc gcgtccggcg cgggcggtca gcacgttaac cgtaccgaat
    15421 ctgcggtgcg tattacccac atcccgaccg ggatcgtgac ccagtgccag aacgaccgtt
    15481 cccagcacaa gaacaaagat caggccatga agcagatgaa agcgaagctt tatgaactgg
    15541 agatgcagaa gaaaaatgcc gagaaacagg cgatggaaga taacaaatct gacatcggct
    15601 ggggcagcca gattcgttct tatgtccttg atgactcccg cattaaagat ctgcgtaccg
    15661 gggtagaaac tcgcaacacg caggccgtgc tggacggcag cctggatcaa tttatcgaag
    15721 caagtttgaa agcagggtta tgaggaacca acatgtctga acaacacgca cagggcgctg
    15781 acgcggtagt cgatcttaac aatgaactga aaacgcgtcg tgagaagctg gcgaacctgc
    15841 gcgagcaggg gattgccttc ccgaacgatt tccgtcgcga tcatacctct gaccaattgc
    15901 acgcagaatt cgacggtaaa gagaacgaag aactggaagc gctgaacatc gaagtcgccg
    15961 ttgctggccg catgatgacc cgccgtatta tgggtaaagc gtctttcgtt accctgcagg
    16021 atgtgggtgg ccgcattcag ctgtacgttg cccgtgacga tctgccggaa ggcgtttaca
    16081 acgagcagtt caaaaaatgg gacctcggcg acatcctcgg cgcgaaaggt aaactgttca
    16141 aaaccaaaac cggcgaactg tctatccact gtaccgagct gcgtctgctg accaaagcac
    16201 tgcgtccgct gccggataaa ttccacggct tgcaggatca ggaagcgcgc tatcgtcagc
    16261 gttatctgga tctcatctct aacgatgaat cccgtaacac ctttaaagta cgctcgcaga
    16321 tcctctctgg tattcgccag ttcatggtga accgcggctt tatggaagtt gaaacgccga
    16381 tgatgcaggt gatcccaggc ggtgccgctg cgcgtccgtt tatcacccac cataacgcgc
    16441 tggatctcga catgtacctg cgtatcgcgc cggaactgta cctcaagcgt ctggtggtcg
    16501 gtggcttcga gcgtgtattc gaaatcaacc gtaacttccg taacgaaggt atttccgtac
    16561 gtcataaccc agagttcacc atgatggaac tctacatggc ttacgcagat tacaaagatc
    16621 tgatcgagct gaccgaatcg ctgttccgta ctctggcaca ggatattctc ggtaagacgg
    16681 aagtgaccta cggcgacgtg acgctggact tcggtaaacc gttcgaaaaa ctgaccatgc
    16741 gtgaagcgat caagaaatat cgcccggaaa ccgacatggc ggatctggat aacttcgact
    16801 ctgcgaaagc gattgctgaa tctatcggta tccacgttga gaagagctgg ggtctgggcc
    16861 gtatcgttac cgagatcttc gaagaagtgg cagaagcaca tctgattcag ccgaccttca
    16921 ttactgaata tccggcagaa gtttctccgc tggcgcgtcg taacgacatt aacccggaaa
    16981 tcacagaccg ctttgagttc ttcatcggtg gtcgtgaaat cggtaacggc tttagcgagc
    17041 tgaatgacgc ggaagatcag gcgcaacgct tcctggatca ggttgccgcg aaagacgcag
    17101 gtgacgacga agcgatgttc tacgatgaag attacgttac cgcactggaa catggcttac
    17161 cgccgacagc aggtctgggg attggcatcg accgtatggt aatgctgttc accaacagcc
    17221 ataccatccg cgacgttatt ctgttcccgg cgatgcgtcc ggtaaaataa gcattacgtt
    17281 atgctcacaa ccccggcaaa tgtcggggtt ttttaattgt gctgcgcgaa agcagacaac
    17341 aattttcttg cttcactatt ggctgcctgc atcaccatcc acggactgaa cgcccacggt
    17401 gtggcatcaa taccgtgtaa tacatctgct aaatcacacc attgataatc catcacttca
    17461 tcatcgttga tctgtaacgc actggtggtg cgtgcggcaa ataccggaca cacttcattt
    17521 tccacaatgc cattcggatc ggtggcgcga tagcgaaagt caggatagat agattcagga
    17581 ggcgtaattt ccacgccaag ctcataacgg caacggcgga tcaccgcgtc ttcgttgctt
    17641 tctcccagtt gtgggtgccc acaaaccgag ttagtccaca cgccaggcca cgcttttttg
    17701 ctcagtgcgc ggcgggtaac taataattgt cctttggcat taaacagcca actggagaac
    17761 gcgagatgta agcgggtgtc tgccgtgtgt gcggcatact tttccagcgt acccgtggga
    17821 actccctgtg cattcaataa aatgacgtgt tccgtttgca taatttctca catgtaattc
    17881 tgatcactca tcattataga taattgaaga gtgaaatgat taaccaagtg agcgtttatc
    17941 gacaaccgcc cgttttgagc ggatgccgac aggtaaaaac catttaaaaa agtagcatca
    18001 tatatttgaa gcgactttca atggccattg caacacagtc agaaatgaca ggaatagaga
    18061 gagtaaaaag caacagcaga ctgaatataa cgcgcattaa aaaattcata ttattctcca
    18121 tgctcatttt tcttcagctt gcgcatcaga agccacatac gtactgtcct gacttttaaa
    18181 tctttatcag agacggattc cttcaccagg tcagcatgat gctggtagcc attaaagaag
    18241 acattcagca caacggcgct aagggttgcg agcataatgc cgctatgcag caacggttgc
    18301 agtacggccg gtaacttaga aaagaaatcg tgagagagcg tcggagtcat gccaacgccg
    18361 agactgatcg ccacaatata gaggttgtaa cggttggtgg tgtagttaca gcgcgacaga
    18421 attcgaatcc ctgtcgccag taccatgccg aacatcacca gcccagcaca aattgcggaa
    18481 tggaggcgac cagcaccgcc atttttggca ccatgccaaa caggattaat ataattcccg
    18541 aagaaataca cacccagcgg ctataaatcc tttcgcatag cgagtaatga gcaagataaa
    18601 aattaagaca gcaaaggaga tacctaaata aacgggatta ccatattgcg gattcccttt
    18661 acctccggcg gcccagtcaa tacccacctg aatgatgcta agcccaatag aagtaataac
    18721 cacaccggta accagtggcg ggaataaagg catcaagcga ccaataaatg gcgctaataa
    18781 tgtggtgata aaacccgcgg cgatagtggc accaaatatc cccagcaggc cgatatccgg
    18841 gttcatccca atggctatca ttggtgttac agcagcaaag gtcaccgaca taatcaccgg
    18901 caggcggatc cccataaagc ggccgatacc gatacattgc aataatgtga cgatcccgca
    18961 gcaaaagaga tccgagctaa tgagcatcgc aatagcatct tttgagaggc ccagtcggtc
    19021 accaatcata agaggaacag cgactgcacc tgcgtacatg acaaggacgt gttgcagacc
    19081 gagtatgatc agctttcctg gtgataatat gcggtcaacc tcatcagttg ggcggtcttg
    19141 cccagaagat gagggaagtt gggaatctat ggcgctcatc agtgtttctc ctttcgcccg
    19201 ccagagtaaa aagcaaccgt ttacgttaat gaaactgtct aaacacatgc tcgtggagtg
    19261 ccagtttttg cgaggaaggg gagttagaga caggaattgc ggaggcaatc acataatcgt
    19321 gcttaaacca cgaaaatgac aagagaacct gacaatggaa ttatctgtaa ttttctcata
    19381 ttgatatttc attaatcatt ctggcatgtc gcactctagc atttaatcgt ttttatctgg
    19441 atagcgctct tttgatcggc aatgttgaaa taatgaaagc ggcatagatt gaggggcaat
    19501 aatttgaact cagtcgcaaa attataatta aaacggtcat gctacctaat atatgcccgt
    19561 ttattgcatc gcgaaatttg agaagacata actcattaaa tgaaagggat gcaaatgaat
    19621 aagtttatcg ctgctgaagc tgcggaatgt ataggctgtc atgcttgtga aattgcctgt
    19681 gcggtggcac acaatcaaga aaactggccg ctgagtcaca gtgactttcg accgcgtatc
    19741 cacgttgtag ggaaaggcca ggctgcgaat ccggtggcct gccatcactg caacaatgcc
    19801 ccttgtgtta cggcttgtcc ggttaatgct ctgactttcc agtccgatag cgtacaactg
    19861 gacgagcaaa aatgtattgg ctgtaaaaga tgcgcaatcg cttgcccctt tggcgtcgtt
    19921 gagatggtcg atacgattgc gcagaaatgc gacctttgta accagcgcag ttccggcacg
    19981 caagcctgta ttgaagtctg cccaacacag gcgttacgac tgatggacga taaagggtta
    20041 cagtggataa aggtggctcg ccagcgcaaa acggcagcag gaaaagcgtc atcagacgct
    20101 cagccatctc gcagtgcagc gttgctcccc gttaactcgc gtaaaggcgc agataaaatt
    20161 tcagcgagtg aacggaaaac ccactttggc gaaatctatt gcgggctgga tccactgcaa
    20221 gcgacttatg agagtgaccg ctgtgtttat tgtgccgaaa aagctaactg caactggcat
    20281 tgcccgctgc ataacgctat tccggattac atccgtctgg tacaggaagg aaagattatt
    20341 gaagcggcag aactttgcca ccagaccagt tccttaccag aaatctgcgg cagggtatgc
    20401 ccacaggacc gtctttgtga aggcgcatgt actttgaaag atcactctgg cgcagtctct
    20461 atcggtaatc tggaacgcta catcaccgat accgcgctgg cgatgggctg gcgtcccgat
    20521 gtcagcaaag ttgttccccg tatcgaaaaa gtggcggtga ttggcgctgg gccagcaggg
    20581 ttagggtgtg ctgatattct ggcgcgcgcg ggagttcagg ttgatgtctt tgatcgccat
    20641 ccagaaattg gcggtatgct gacttttggc attcctcctt tcaaactcga taaaacggta
    20701 ttaagccagc ggcgagagat attcaccgca atgggaatcg actttcatct taactgtgaa
    20761 attggccgcg atattacctt tagcgattta acttctgaat atgatgcagt tttcatcggc
    20821 gtggggactt acgggatgat gcgagcagat ctgccgcatg aagatgcgcc cggtgtcatt
    20881 caggctctac cgttcctgac tgcccatacc cgccagctca tgggattgcc ggagtctgaa
    20941 gagtatccgc tgacggacgt ggaaggtaag cgagtcgtgg tattgggcgg tggcgatacg
    21001 acaatggatt gtttgcggac ttccattcgc ctcaatgctg ccagcgtgac ctgcgcgtat
    21061 cgtcgtgatg aagtcagtat gccgggctcg cgcaaagagg tggttaatgc gcgcgaggaa
    21121 ggtgtcgagt ttcagttcaa tgttcaaccg caatatatcg cttgtgacga agatgggcgt
    21181 ttaactgcgg tgggcctgat tcgtaccgcc atgggtgagc cggggccgga tggtcgccgt
    21241 cgtcctcgtc cggtagcggg ttcagagttt gaattgcccg ccgatgttct gattatggcc
    21301 tttggtttcc aggcgcatgc catgccgtgg ttgcagggca gcggaattaa actcgataaa
    21361 tggggcctga ttcaaaccgg tgacgtcggg tatttaccta cccagacgca tctgaaaaaa
    21421 gtctttgctg gtggtgatgc agttcatggc gcggatctgg ttgtcactgc aatggccgca
    21481 ggaaggcagg cggcgcgcga tatgttaact ctgtttgata cgaaggcatc gtgatgaaat
    21541 cgttaattat cgttaatccg gctgactgta ttggctgccg tacttgtgaa gtagcctgtg
    21601 tggtcgctca cccttcagaa caggagttga atgccgatgt ctttttaccc cggctgaagg
    21661 tacagcgact ggacagcatc agtgcgccgg tgatgtgcca tcagtgtgaa aacgcccctt
    21721 gtgttggcgc ttgccccgtg ggggcgctga cgatgggcga gcaggtggtg cagaccaatt
    21781 ctgcccgttg tattggctgt cagagttgcg tcagtgcgtg cccgtttgga atgatcacca
    21841 ttcagtcgtt gccgggggat acccgacaac aaattgtgaa atgcgatctc tgtgaacagc
    21901 gggaagaggg gccagcctgt gttgaatctt gcccgacaca ggcgctgcag ttgcttactg
    21961 aaagagaact caggcgagtc cgccagcagc gtattgttgc cagcggtgag aatcctctgt
    22021 gaaagaaatc tccgcgctga aaggcgcgga gaatctatta gattgcccaa ccacccgcgt
    22081 aaaatgcgac cagtgcgaca ggaacgctaa tttgttgccc gagtaccaga ctgaatgcgg
    22141 taaatgccgt caaggaaatc gcacaaccaa tcgccattgg caaattggcc tacaacccca
    22201 tcagcaacga gccgaagccc gcgaccagac aggtggcaac aaacacagct gcgggaggaa
    22261 aacctgcttt gcccagcatt cccggaacga cgataacgga ataaaccatg gccagaaagg
    22321 tcgttaagcc agccagtact tcctgacgaa cggtactgcc acgagcggta attttaaaat
    22381 aattatccag cgcgccctgt ggctttggtg cgtccggtgt ttgtaggatg tctccagaca
    22441 taatgatgtc ctcacgagga tgttaaagtt ttattattag ttgcgttcgt acaccagacg
    22501 accatcaacg taggtgcggt agatcgaacg gtcatcgccc aacgtcatca tcacgaacaa
    22561 tttgtcgact aaagaaacag agttgtcata gcgcagctgc tgtagcggag tggcggtggg
    22621 ttccatcacc acgaaatcag cctctttgcc aggtaaaaag ttgccaatca aatcgtcaag
    22681 gcccagagat ttcgctccgc cgagcgtggc caggtaaaac gcttcatatg ccgagaggcg
    22741 atagccttgt aattgcaaca ctttgtaggc ttcgttcagc gtttgcagca tgttgaaagt
    22801 tgttccggca ccgatatccg ttcccatgcc cactttgact ttcttctgcc cagatgccgc
    22861 atgcatcgtg aattgcggta gcaatcgccg gagctgcgcc atttacaccg atttccgaga
    22921 tcgatttcgc cccgaacggg ccgactttat cgtcgctcgg caccagcaca gcgcggaaat
    22981 cacgcggaat gtcaccaatt ttcggtgcgc cgtaactgcg taaatcacgc gttaacgggt
    23041 gaccttcggc gtcataaatg atctcttcgc tcatactgtg gccgatagct cgcagggtag
    23101 caccgtagat ttgtcccaat gctaattccg ggttgaccgg tgtaccgcag tccagcaagg
    23161 cgtagaattt atccaggcgg atttcacccg tacgcgtgtt gacggcaact tcagcgaagt
    23221 ttgcgccata cgggaaggcg aaatcaggcg tgatataact gccagttccc accagtgaac
    23281 caaagccgat gccggtttcg cctttatggg caatatcccc gaaactaact tcgcctttct
    23341 tgccgcgcac gacgcccggc gttgccagtt gaacatctgc cactgcctca cctaacattt
    23401 gcgcgccgtg gaacagaatt ttctcccgta gattttccgc tgccaaacgc gccgcgttac
    23461 ccgagaagca agtaccggac gaggcatatg cgcctttatc aaacaacgca tgatcggtat
    23521 caccggagat aacatgcacg tcctgcggtg ggcagtgcag cacttctgct gccagtttcg
    23581 tcactacagt atccagacca gtaccaatat ccgcaccgcc agaatgaacg ataaaggtgc
    23641 cgtccgattc cagtttgatc atgcagttag cctgatcgat atccgggata cccgatttct
    23701 gcatgataat ggcgacgccg cgaccgatgt gccagtcacc attttgcggt ttcggtgacg
    23761 accattggat catctcgcga ccctgacgca ggatctcttc cagtgcacag ctggcggcgg
    23821 aaggaacgga ggtcggcgct ttaccttcac cgattgcacc gagaattttc agctcttgcc
    23881 cttcgtgtac ccggttacgt tcgataattt ccagttggtc gatctgtaac tgttcagcca
    23941 gttccgctaa tgccatggtg atagcgaagt tacctttcgg tgcgccataa ccctgataag
    24001 caccatttgg gcaaatgttg ctgtagtagg tggtgacctg gaaatcgacg ttatcgcacg
    24061 gatataacgg cagcgacagc gccggtccgt tacacggtac ggtgagtgag tggttgccgt
    24121 aagggccagt gttggcgcgg aaatccatct tcactgccgt caggcgacca tcttttttcg
    24181 ctcccagttt gacggtgact ttcgcgacgt gacgagaggt gttagcaata aactcttctt
    24241 cacgggtgta gcggaatagt accggacgcc cggtcacgca ggttgcccag gcgcacactt
    24301 cttccagcag gatgtcctgt ttggaaccaa aaccgccgcc aactcgctct ttaatgacat
    24361 gaactttatg ctgtttcatg cccacgaggc gcgcgacctg gcggcgtaag tgccatggta
    24421 cctgggtgga ggcgtggata accagacgat cgccgtccat ccgggtaaag cagatatgtg
    24481 tttcagtcgg gcactgctgt gcctgcgttg agttataggt tcgctcaatg atcacatcgg
    24541 catcggcaaa gcctttgtcc atatcgccaa tatgaccatg aatactggcg gcgatatttt
    24601 tgcgagggcg agaaccgatc gggaagttga tgatcatatg ctcgccgcgc tgggctgcat
    24661 ggctgttatc gtcttccaga gtatctggcg caccagcaac atacaccacc ggttcatcgt
    24721 gcacgacagg cgcatcttcc gccattgctt cgtcgatcga cattaccggc ttaagcactt
    24781 catattcaac gtcgatgagc ttcagtgctt cgagcgcaat ttcttcactt tctgcgacga
    24841 ccgcagcaac acgatcgccg acgtgacgca ttttcttgcc gaacatacgg cggtcaagcg
    24901 gtgacggttc cggtgcgctc tgaccccccg gggtatagta gatatcaggg caattcaggt
    24961 gagtaataac gtgaacgacg cccggtaagg cttcagcttt gctgacatcc agatgagtaa
    25021 tcagtgcgtg agcgtgtggg ctacgtaaca ttttaatgac gcaggcgtca gccgttacgc
    25081 agtcttcaac atagcagggt ttcgcctgca ccattttcgc ggcatcagtt ttaggataat
    25141 gcttgccaat gacgtcaagg tcgtcacgga aagtcggagc gatatcaatg gtggcctgcg
    25201 gattattttt acgtgcaacc gccagttcaa tgacctgata gtattgttgc cagcctgcat
    25261 cgcggctgaa caaaccagaa agcgcgtcgt cgatctcttc gcgggtaggt gcggcgatgc
    25321 gatcgagaag atcggtgata atcagagctg cagctggatc gttataacca gactgcacca
    25381 cgccaacatc aaccattgcc tgttgaacca gacttaactc gttccattta cccagagatt
    25441 ctgcggtacg aatatctgcc ttctctaact gtgcggcaat aagcaaggac gcgttaacga
    25501 tattaccgtt aaagattatt gcgtcagaac cggcaaaccc gaagccatca tcactgttgc
    25561 gtacagagtg cattcccatg ttaaacaaca gcttttgcac gttttcgcct ggattaacgg
    25621 ttagctcctg aggcgcgcca tttaaagtaa agtggatgat catacggctt cctccccagc
    25681 ctgttggcag tcggcataca ggtcggctac cagaactccc gtgatatagc gtttataggc
    25741 cacgctgccc cgcaaatctt cctgcgggaa aatggcgttg gcgacagcct gttcaagtgc
    25801 attgccttcc agattttgtt tttcgacatc gtgcagacgc agtgctttac tggcgacgcc
    25861 atccagcgca attcgcatac cgtcgtggtc tgtcattgca acggctgccg tcacgacggt
    25921 taaacctgcc tgagaacggc taattttgcg ggtcgcacag gtgcgatacg gatctttaat
    25981 gataatttcg gttagcaggc gatcgcatgg gcaggccagg tagtcctcga ttgacagcgt
    26041 ttcgccgttg ccaaaaacca gttcagcatc cagtgctagc aggacgggaa gcagcactga
    26101 ctcttcctgc cgggcggcga tttcaccacc aatggtcgac tgattacgaa catggcgtga
    26161 gtaaacaaaa ccgagggctt cacgcagtgc tgcaggaata aagcgcgcat cacgcagtgg
    26221 ctgcaagcga gacattgcac caatccgcag tgcaccgtta tcccagtcaa cccagtccag
    26281 ttccagatcc tgtaaggaaa tggcaatctt tttatcggta cgggttggtg tagcattgag
    26341 tttgctgccc ccggcgaacc agacggcttc atcctggtag cggcgcttca gttccagcgc
    26401 ctgttcgaca gagtcgggcc tgaaaaattg ttcaatcatc ttctttcctt atttaaccct
    26461 cttccctgcg ggaagagggc atctgtcatt tatgccagcg catccatccg acgccacata
    26521 ctggcagcgg cttttctggc ttgtgcataa atggaatcgc aatcgaagtt aaactgacgg
    26581 tcttcataga ccatcacacc attgaccatc acgctgtgaa cgctgcctga gcccataccg
    26641 aaagcgatat gcccggcgat attgtctgcc agcagcggcg tcggcgagtt gtaatcgcaa
    26701 atggtcaaat cagctttgta accggcttcc agaagcccaa atttcgcgcc aaagttgcgg
    26761 ctcatcagtt cgttaccgtt agtcagggct ttggcaaaac tgtcaggcca cagcggacca
    26821 cccgcatcgc gatgtttaaa gaaggcaaat ttcatctctt caaacatgtc cgaaccaatg
    26881 ccgtccgttc ccaatgccag gttgcggatg tcgctaaggt gatggttgta gccgacatgg
    26941 ttgttcatgt ttgaacgggc gttatgcacc aggaacgcat cgcgctgatt gagtagggcg
    27001 atgtcatctt tcgacaagta cagcccatga gcgaccagcg ttttgctgtc gatgaggtcg
    27061 aattgcgcca gtcgtgccag caggtctttg ccgtaccagt ggtgactgta ggaaacgtcg
    27121 taaaggtctt ccgcagcgtg aatatgcaaa ccacggcctg tggctttcac ggcttcacgc
    27181 agcatctcca gaccggcatc cggcacggta aacggcgcgt gagcaccgat atgtgcttcc
    27241 accagatacg gctcgcttgt cgctttcttc gcctcatcaa tcaaacgggc gaaacggatg
    27301 ttttcttcta caccttcctg caactctttg atgccgttgt tacggtcagt agtttcaaaa
    27361 caggtcatcg cgcgcaggcc aacttttaaa aatgcgtcgc gcaatgtgga gagcgacccg
    27421 ccgatatacg ccggcgaggc atggtgatcg ataaccgatg tacatccgct cttaatcgct
    27481 tccagggaac aaatcagtcc gctgtaatag agcgactctt catcaagggc gcgatcgagc
    27541 cgccaccaga gatttttcag cgttgagatg aaatccgggc aaggggcgat gtttgccata
    27601 attccgcggg aaagctccga gtaaaaatgg ttgtgcgagc agacaattcc cggcatcaca
    27661 atccgaccat gcatctcttt gtagctggca tcggggtagc gttgcgtcag ggcatcgccg
    27721 atagcgacaa tcacgtcatt ttcgatagcg atatcaacgc cttcctgcac tttcgccggg
    27781 tgtaactgca ctgcggtgac attcttcaga atcaacatga ttacacctcc acgcggccca
    27841 gcagataatg atgatgctgg tggacatggc tgatgatgcg gcacatatcg ttcagctccg
    27901 gtggtacgtt gttaaactga ccttcgctgt cgatgtttaa cacccagctt tggttattca
    27961 gacgtactcg tacccggcaa tcttccacaa ggaagcctgg ggtgctgctg ttatcaaagt
    28021 cttgcgccag gctgaagacg gtgattttgt cttcgtatgg tttaccgttc cacggacaga
    28081 actgagcgca gttgccgcat tcgttacagt aagcgtcgag gtgcagcgtc tggaatctat
    28141 tctggaagcc agggactgca atggagacgt tggcgcggtt cgggcagaca tccacacact
    28201 tgttgcaaac gtagttacat tcgaggcagc gagcggcttc ctgggcgaca aacgcgtcac
    28261 gatcgtcact gttcaccagc gtgatagaga tatcgccttt acgttgatag atttccgctg
    28321 gattgacgtt gttccagtat ttatcgttct ggtgggaacg gatattttcc cgactaagga
    28381 tggcatcggt cgcccgacgc gcggttccga cagcagcgac aatggaggat ggtccgcgct
    28441 gtacgtcgcc gatcataaag acgtcagtca gacgagtttc gccattatgg tcgacgtctg
    28501 gccagccgtt tttgtccagc ggcacgccca tcgcattcag ggcttcagta tcctgctgtt
    28561 caccaatggc ggtgatcagg ctgtctacat gcagtgttac tgtttcgttg gtttcaaccg
    28621 gacggcgacg acctttctca tccggttcgc caagcgacat aacgcgcaag gttaaggtgc
    28681 catcagcatc gaaacgttcc ggattattca ggaaacggaa ctctacgccg tcgtgcaacg
    28741 cttcttcata ctcttcgcgc catgcgggca tctcttgtag tgaacgacgg taaacgatcg
    28801 ttgctttttc tacgcctgga acgcgtaacg ccgcacgagc gcagtccatt gcggtgttac
    28861 ccgccccgac aacgaccaca tgtttgccca gcttgagcgc tgtacccttg ttgtattcac
    28921 gcaggaaggg gagtgatttc cagacatttt ggttgtcgcc cgccagtttc acaccgctat
    28981 ttttatcagt gccggtggca atcagaacat agtggaagcc ctgatttttt aactgttcaa
    29041 cggttaaatc gggtgagcag ccatactcaa atttcacgcc gtgagcggca acaaaatcga
    29101 tatcgtgctg aattaactct gcaggaatac ggaactgagg aatgatattt ttcaccacgc
    29161 cgccagcatt ggcttcgcgt tcaaacagcg taaccggatg gcccgctctg gcaaggaagt
    29221 aacctgctgc cagacccgcc ggacctgcac caatcacggc aaccggatgg cgtgaaccag
    29281 aaccggcggg tttgtgccag cgttgcttat attcatccca acctttttcc agcgcgactt
    29341 ttttcagttc gcggatattc agcgcactgt cgtaatccag gcgggtacag ttgtattggc
    29401 actggtgatc gcaaatatga ccggtaatgg caggtagggc gttgcgttgg tagatgagtt
    29461 ccagcgcgtc ggcatagcgg tgttcgccaa gcagacggat gtattccgga atatcttgct
    29521 tgatagcgca ggcagtaaca cagggggcaa cgtagcaatc ggtcagcggc aggtcttctg
    29581 ccacttcaat acgctcttct ggcttccagt gtttctgggt gtattccatg gttaacgcgt
    29641 ctgctgccag tctgttcagt cgttcgacgt caacatggtc aagtccccag gcgtcggagc
    29701 cttccagctc gcgcatgcag gcacttaagc gcagatagcc gccaggtttc agcaggtcgg
    29761 ttgccatagt aatagggcga atacctgtat caaaaatatc gcggatagtc agctgactgg
    29821 caccaccgga ataagaaatg ggcagtttgc cgtcaaaggc gcgagagaga actgctgcaa
    29881 cattgatgga gagcgggaac agcgcacggc ctgacatata catctcttca ccaggcagtg
    29941 cgcctttatt gttgatggtg ccgagagtgt tagtcagttt tacgccaaag ccgagtgatt
    30001 tttcttttgc cagtaccatc aggcgctcca gcatttccag tgcttgcgtc agcttgaggt
    30061 cgtgatcaaa tgactcttct tttaagccta tgtaaccgaa accgcagaca tcgaggatct
    30121 cacgaacacg cgcgtacccc agtaaggtcg ggttaagttt gacaaaggtg ttgagccctt
    30181 tttcttccag catgtagcgg caaatggctt cgatttcatg cggaggacag ccgtgcatgg
    30241 tggagagggt gacgccctgc accatactgg tggggatgcg agcgggtaag gcttgcaagt
    30301 tttcgcgttt ttcctgcaat ccgtggcggg ctaaaaatgc gtcatcctgg agtaatttat
    30361 tcagcgtatc gcgatattga gcgaatttcg gatggtcaga tgcgtccatc atattgtcga
    30421 tgaactgttg catcggcggt tgcttaatac cttcgaggtt gtaaccgacg ctcatattaa
    30481 agatgaacga tttaccggaa tcagaaggct gtaacatcgc ttcgagaagg tgcagggcaa
    30541 accaggcttt gaggtattca tcccaggctt taagcaaggt aaattcggta gaccattcgg
    30601 tgttaaagca ttcgtcttcg gcatcaatac agggcttttc cagctccagg cggtcaagaa
    30661 tttggacggt ttttagttcg atgaagcgtc cgccagtcag ccaggaagtg acaatatttt
    30721 gcgcgagctg cgtgtgcggg ccagcggcag ggccgacggg agtggcacag gtttcaccga
    30781 agacgctaac agttttacct tttacaggtg agtaaaactg ttgctcggga ataccaaaga
    30841 ttgagcgttg ttgttggtat tcatcaaata tgcgcgtcaa aagttcctca aacggaatgg
    30901 gacgcataat atcccccata accctctcct tagcttatac agatatcatt gagtgaccgg
    30961 tgttgagaaa tccggtggtt tagggggata gagcaacaat cgcaccactc ttgccaggtt
    31021 tggcgggaaa tataaaaaaa tgtgaggtgg ctcgaaactt aaaggctttt tgtgaatgaa
    31081 ctcacaggtg ccaaatgaga attaaaatca ctcctgaaaa ttattattgt cagcccattt
    31141 atcacttttt ctcttttctc aaaatcagtg attccttatc attatgataa agagtgctgg
    31201 agagattaaa tatttgaacg caatcgttta actacatttt ctaaatttta gtataccttt
    31261 tttgcgctgt aataaagtcg tccggagtat caatatctaa aattatttct gcattttcta
    31321 tttcgacgga ataatgatct cccataagga gtgcctgacg catattggtg acattgggtc
    31381 gctggattgc ctgcatcagg catggttttg acactaaaat cggatggccg gggatgccat
    31441 tatggagtgg cagtattgcg ccatcatttc gtaacgacca gatttttcta aaaatatcga
    31501 tggtgagggt tggcatgtca ccgtgggtga gaaaacaatg ttctgtttgc actgcgggta
    31561 ctgcggcctt gacggatgtt agtaaaccct gcgcataatc tgggttgtga ataatagtga
    31621 tattgctctg gttcgcataa cgttcgtgca gttcattacc acgatagccg gtgactaaaa
    31681 taattcggct acaaaactgc aacgcatttt tgatacttgt atcaagaatt gttccctgtt
    31741 cccaggggaa catcattttc cattgcccca ttcttgatga taatccggca gcggtaatta
    31801 tacagtcgat ggctgacata gtggttccat tatttatagg ggctaataac gcctgataat
    31861 tgcgttactt cctgatggat taagttgggg ataatttatc ccagagaggt cataaagact
    31921 cgtgaaaagt atagttgacc catcggcatt agtcattgat ttaggtgcgc agaaacgtcc
    31981 cactgtgatt tctgttgttg gtgctggggg gaaaaccagc ttgctttttt ggctggcaga
    32041 actgctccag gctagcggca ggcgtgtgtt aatcacgacg acgacacata tgtttatgcc
    32101 aacgtctcac tggcccgtgg ttttctgtcg tgatcccgcc atgcttcctc atgcgtctct
    32161 tacatccccc atttcatttt gttttcacag ctggaaagcg aaccagggaa aagtgcaggg
    32221 atttacgcca gaagcgattg atgcactggt gcaacgacca gagtgtgacg taattctcat
    32281 tgaagcagat ggctcgcgtg gaatgccgtt aaaagcgcct gatgagcacg aaccttgcat
    32341 acccaaaagc agttgctgcg tgattgctgt gatgggagga catactttgg gcgcgaaagt
    32401 gagcacagaa aatgtccatc gctggtcgca gtttgctgat attaccgggt taacacctga
    32461 tgcaaccttg caactgagcg atctcgttgc gctggttcgc caccctcagg gggcgtttaa
    32521 aaacgtaccg caaggttgtc ggcgagtctg gttcattaac cgtttttctc aatgtgagaa
    32581 tgcgattgca caaagcgagc tccttcaacc gctgcaacaa cacgacgtag aggcaatctg
    32641 gctgggcgat atacaagaac atcctgcaat cgcgcgcaga tttgtgaatt agcggcccgg
    32701 gtattccggg ttactgaaag gatatccatt gaggttggta tgaatatttt cacagaggct
    32761 gcaaaactcg aagagcaaaa ttgtccgttt gcgatggcgc aaattgttga tagccgaggc
    32821 tcgactcccc gccattctgc acaaatgtta gtgcgcgccg atggttctat cgtcggtaca
    32881 attggtggcg gaatggttga gtggaaggtg attgaagagt cacttcaggc attgcaggaa
    32941 cgtaagccgc gattattcca tggacgtatg gctcgtaacg gtgcggatgc tgtcgggtca
    33001 gattgtggag gtgcaatgtc agtatttatc agcgtccatg gtatgcgtcc acgtctggtg
    33061 ttgattggcg cggggcatgt caaccgggcg atagcccaga gtgcggcgct attaggattt
    33121 gatattgccg ttgccgatat ttatcgcgaa agcctcaatc ctgaactatt cccaccatca
    33181 accacgcttc tccatgctga gtcgtttggt gcggcagtgg aagaactgga tattcgccct
    33241 gataattttg tcctgattgc cacgaataat caggatcgtg aagccctcga caaactcatt
    33301 gaacagccca ttgcatggtt ggggttgctg gcaagtcgcc gcaaggttca gcttttcctg
    33361 cgtcaattgc gtgagaaagg cgtggctgaa gaacatattg cccgtttaca tgcgcccgtt
    33421 ggttacaaca taggtgcgga aacgccgcag gagatcgcca tcagcgtgct ggcagaaata
    33481 ttacgggtga aaaataacgc gccgggtggg ctgatgatga aaccttctca tccttccgga
    33541 caccagctgg tggtgattcg cggtgcggga gatatcgcca gtggtgtggc gctacgtctg
    33601 tatcatgcgg gttttaaagt gatcatgctg gaagtggaaa aaccgacagt gattcgttgt
    33661 accgtggcgt ttgcccaggc cgtgttcgat ggcgaaatga cggtcgaagg cgtcactgct
    33721 cgcctggcaa ccagctctgc ggaagcgatg aaacttaccg aacgcggatt catccctgtg
    33781 atggtagatc ccgcctgttc attgcttgat gaactgaaac cgctttgcgt ggtggacgct
    33841 attctggcga aacagaattt gggaacgcgg gcagatatgg caccagtaac aatcgcgctt
    33901 gggccgggct ttactgcagg gaaggattgt catgcggtaa ttgaaacaaa tcgcgggcac
    33961 tggctcggtc aggtgattta ctctggttgt gcgcaggaga ataccgatgt tcctggcaat
    34021 attatggggc ataccacccg acgggtgatc cgtgctcctg ctgcaggcat tatgcgatcc
    34081 aacgtgaaat taggcgatct ggtgaaagag ggcgatgtga ttgcctggat tggtgagcat
    34141 gaaattaaag caccgttgac ggggatggtg cgtggcttgt tgaacgacgg cctggcagtg
    34201 gttggtggtt ttaaaattgg tgatatcgat cctcgtggtg aaaccgctga tttcaccagc
    34261 gtttctgata aagcccgggc gattggcggc ggcgtacttg aggcgttaat gatgttgatg
    34321 catcagggcg tgaaagcgac aaaagaagtg ctggaagtgg cttaaatgaa aatggccgga
    34381 tggtattacc cacccggcct taaaattact gacaaatcac cgttccggtt ttgccttcaa
    34441 tcccttcttt cgctttgctc aatacggtaa tcagtgcttc acgacctgcg cgagagcgag
    34501 caaacgaggc ggcagcttcc actttaggca gcatagaacc tttcgcaaaa tgaccttctt
    34561 cgataaagcg ttcagcatca cttaatgaca ggcgatcgag ccactgttca ttctctttac
    34621 cgaagttaat ggccactttt tctaccgcag tcagaatgat cagcatatcg gcatcaatca
    34681 tttctgctaa acgggcgctg gcccagtctt tatcgataac cgcgctggca ccacgcagat
    34741 ggttaccttc acgaataaca gggataccac caccgccaac ggcgatcacc acctggcctg
    34801 catctaccag agctttaacc gtttcttttt caatgatatc aaccggtttt ggcgaggcaa
    34861 caacacggcg atagccgcga ccagcatctt ctttcagagt gtaaccctgt tttgtcagtt
    34921 gctctgcttc ttgctcggta aagaacgaac cgatcggctt ggtcgggttg aggaatgccg
    34981 gatcgttagc gtcgacttcg acctgagtaa ccagcgttgc tacaggttta ttaatgcccc
    35041 gagaaagcag ttcttctctt aacgcgtttt gtagatcgta accaatataa ccctggctta
    35101 aggcaacaca gacagacatc ggcagcattg gcgagtgtgc ttcagttttt gcggcagctt
    35161 caaaggcttg gttaatcatg cccacctgcg gcccgttacc atgagtgacg ataacttcat
    35221 gtccttgggc aattaaatca acaattgcct gagaagtaat ttttaccgct ttcatttgtc
    35281 cggccaggtc atcgcccagc gcattcccgc ccagggcgag aacaattttc ttactcattt
    35341 ttcctcactc atcattcttg attgtgtata gatccctgac agtggttaga gcacgggagg
    35401 gacaaacggt ttgcgtcgca ggaaacgacc tcgcccggct ttgcctgtaa aggtgccgtc
    35461 acagaaaatc gtttcaccac gggataaggt tctgacaatc gcgccctgac aggtaaaacc
    35521 ctcccatggc gagtagtcgg cgttgtcgtg gagatggcga tgctgaattt gttggctctg
    35581 acgtgggtcg ataatcacca cgtcgccatc ggaaccgggc gctaataatc ctttttgcgg
    35641 ccacaggcca aacagcctgg cgggcattgc gctggttaat tcaacaaagc gttccggtgt
    35701 tatacgtccc gtcatcacgc cactggaaaa cagtaactgc atgcggtttt ccacaccggg
    35761 taagccattt gggcagcgac tgaaatcgcc tttagaaatt tgcaggcgtt gagccatcga
    35821 gaaggtgcag tgatcggtcg ccaccacgtc aatcgcacca tcgctgatgc cacaccacag
    35881 tttgtcctgc tcgcgtacgt tacgcagcgg tgggctaaga atgaacttca tgccatcttc
    35941 tgtatcgtaa ctgcgttcgt ccaacaggag atattgtggg caggtttcaa cccagactgg
    36001 ctggtgattc gcacgggcaa gacgcagata atccagacct aagccgttag acaggtgcac
    36061 gatatagagc ggggcgttac cggcaatttg tgccaggtta atcatgcggg cgatggcttc
    36121 cgcttcgcat tccagagggc gactcaaggc atgatagcgc ggcgcggtta accctgcggc
    36181 gataaattcc gcccgcttgc tggcgatagc cgcatcattt tccgggtgca cggtggtcag
    36241 cgcgccggat tcatgcaagc ggcgtaatgc ctgcaaaacc tcgtcatcgt tgagtttgta
    36301 ttgataggtt aaatagagtt taaaactgct cagtccttcc tcgaccatca tcgggatttc
    36361 gtcgaggatt gcgtgattaa tgtgctggat cacaccgtga aagctgtaat cgatgaccgc
    36421 tttatgggcg gcataaccac gataaacctc cagttgatgg cgtaaccgac agccgtttgg
    36481 gccaaatccc atatggtcaa taatggttgt tgtaccgcca cacgcagctg cgcgggtacc
    36541 ggtaaaaaaa tcatcacaac tgcgcgcgat gccgacatca atattgaaat gcgtatggac
    36601 atccacgccg ccagggaaaa cgtaacagcc agtggcatca atttcttcat acgggagctg
    36661 cggcgaaata ttgttgccca actggcgaac aatcccgctt tcaatcagca aatcctgttt
    36721 ggcttgtcca tctgcgttaa cgacagtgcc gtttttgatc aatacgcgca tagcaaactc
    36781 caaaaaccct ccggcgaacc ggaggggaat aagtgaagag taattactct gttgccagcc
    36841 agcttaacgg gattgcagcg tacatcgcgg cacaggtcac caggtgagat ttccaggttt
    36901 tttcgttagg tgcatgcgct tcaggttctt tacccgggcc aaagccgatc accggaatgc
    36961 cgtgacggcc catgatagat acgccgttag ttgagaaggt ccacttatca acaaccggcg
    37021 ctttgccaaa cagcccttcg taggcattca ccagtgcttt aacggtgaag tgatcttctt
    37081 ccactttcca ggtcgggaag tagcattcgg ttgggtaaac caggccagtc caggacggac
    37141 ggtcgtagtt gtacatagaa acaaccgcgt tagctttctg tactgcaggc agggcgcgga
    37201 tttcgtccag cgcgccttcc caggtttcgc cccaggtcag acggcggtca atagagacgg
    37261 cgcagctatc tgctacagcg caacggcttg gggatgtgaa gaagatttca gaaacggtga
    37321 gggtgccttt gccgaggaat tcgtcataac ccagacgttg ggagagttct tgtaattcgc
    37381 caagaatcgg acccattttg aaaatggcgt tgtcaccgcg ttctggcgca gaaccgtggc
    37441 agctaacacc ctgaacatca atacgaattt ccatacgacc gcgctgacca cggtatatct
    37501 ggcagtcggt tggttcggta ctgaccacaa attccgggcg aatgccggat tgttcaataa
    37561 tgtactgcca gcacagaccg tcgcagtctt cttcctgcac agtaccggta accagcaggg
    37621 tatattcatc ttccagaccg aggtctttaa tgattttacc ggcataaacc atagatgcca
    37681 tgccgccttc ctggtcggaa gtaccgcgac caccgattag ctcatcagtt tccatgcctt
    37741 cgtacggatc gaagtcccag tttttgatgt tgccaatgcc gacggtatcg atatgagcgt
    37801 ccattgccac cagacgcggg ccgtggccga tataaccgag aacgttgccc atcgggtcga
    37861 tttcaacttt atcgaagccg actttttcca tctcttcttt aatacgatgt actacgcgtt
    37921 tttcgtcgca gctttcactg ggaatagcaa ccatgtcgcg caggaagcga gtcatgtccg
    37981 cctggtaatc ttttgctttt tcaagaatca gtttgaatgg aatattctta gccattattt
    38041 ttctccagat tttttactcc ccgttttgca gggagtagtt ccaatagggt gattaaggtg
    38101 ctacagcgtg tttgccttcc cagacaactt cgcggtagtg cttcacgtcg gtgtcgcctt
    38161 cagtgctgat aaccagcact acggcatctt tgtccagcgc cagtttttcc atcaggcttt
    38221 gatgttgcgg gtgataatga accgctgcga gaacgcccaa accgacagcg ccggattcac
    38281 cggagatgat gcgcgggtcg ttgccgtacg gattacccag cacgcgcata cctaatgcgg
    38341 caacgctgtc ctggcaggag atgaattggg tggcgcagtt acgtaggatt tcccagccca
    38401 gcgggttagg ttcgccacag gccaggcccg ccatgatggt ggccatatca ccgccaacgt
    38461 tgacgatgtc gcctttgacg ccggagcgat aaatacagtc agctttgtca ggttcaacaa
    38521 taatgctgtg cagattttgc gggctgtaga cgtcgaccag ataacccagc acaccaccgg
    38581 ccattgctcc gacaccggct tgcagcagaa cgtgcgtcgg ggttacgccc atttcacgca
    38641 tttgctcgac ggcttcatct gccagggttg cgtagccttg catgatccag gttgggattt
    38701 tggtgtaacc ttcccatgcc gtgtcctgta ccacttccca gccgtgctgc tgcgcgtgtt
    38761 gcatggtcag gcgaacggta tcgtcatagt tcatatccgt gacgatgcac tcggcaccga
    38821 ggttcagaat ggcgtcaacg cgttcctgag cagaaccttt cggcatgtaa atcaccgcat
    38881 tctgtccgag ttgctgtgct gcccacgcca caccgcgccc gtggttgccg tcggtggtgg
    38941 tcgcgaaagt cattttttcg ccgatggcat ttttcaggtg ctcaaatgac agcgtttcga
    39001 tatcaagatg atatttttca cacaataatt gagcgatggc gtacgcaccg ccaagcattt
    39061 tgaaggcgtt cagaccgaat cgttttgatt cgtctttgac gagaattttc ttcacaccaa
    39121 aaaggtttgc gagatcgtcc agcgcacaaa gcggtgttgg gcgataacca gctattttct
    39181 ggtggaactg gcgcgccagt ttggcctggc tttgcgaaaa gagcggtgat gtttcgccgt
    39241 tgaaaaattt gttatcggcg atatcaatct tcaatgagaa aacggacata tcctatcctt
    39301 ttggcctctg gaaatagagg gccgaccagc agatcggccc gaggggttat ttcacgcgtt
    39361 cttgcgcttc tttcagcagt tgctcgagca gtgcacctgg tttggcgtat ttacgggaca
    39421 ggatcatcgc ggcgatgata taaggcttcc agctggcttc tttgtaggta gcgatacggt
    39481 atttttcgaa tacgccttca gtcacttcac cttctttgca ggatacgccg ctgatatcag
    39541 ctggcaggca gtgcatgtac agggcttcgc catcacgggt cagttccatc atctcttcag
    39601 tacaatgcca gtctttgtgt tgcgcgttct gtgccagaca ctgtttttcc agtgctttta
    39661 agccttcgtg gtcgttcgca cgcagcaatt cagtacgctc ttccatcact ttgtaaggtg
    39721 cccatgactt cggataaacg atgtctgcgt ctttgaaggc ttcttccatg ctggtgacct
    39781 gacggaagct accaccggag gctttagcat tgtttttcgc cacttcaacc acatccggga
    39841 tcaggtcgta gccttccgga tgggccaagg tgacatccat accgaagcga gtcatcagac
    39901 cgatgatgcc ttgtggtaca gagagcggtt tgccatagct tggagagtag gcccaggtca
    39961 tggcgatttt tttacctttc aggttttcca gtgaaccaaa gtgttcacgt aaccacgcga
    40021 ggtcagccat tgactgagtc gggtggtcaa tatcgcattg caggttcact aaagccggac
    40081 gctgtggcag tacaccctgc ttgtaaccgt catcaagtgc agcgccaact tcacgcatat
    40141 aggcgttgcc tgcgcccaga tacatatcgt cgcgaatacc aatagcgtcg gcgcagaagg
    40201 agatcatatt ggcggtttca cgcacggttt cgccgtgagc gatttgtgat ttgccttcgt
    40261 cgagatcttg ttgtgcaagg ccgagcaggt taagcgcgga agcataagag aagcgggtac
    40321 gggtggagtt gtcgcggaat acggaaatac ctaatccact attaaagact ttggttgaga
    40381 tgttttcagc acgcagtgct tttaatgctg cggcaacgtc cagtacttgt ttcagttcat
    40441 ctggcgtctg ttcccacgtt aacaaaaaat ctttctcgtg aaggtgagag gtcagcgaat
    40501 tgatatcctt aatcagctca ttaacagttt tcatcataca gtcccggtag tggaatgaca
    40561 ggcaaagtgg tttatttcac tgtgacttgc caaagtgatt ggtgttttca tagctatacc
    40621 tgggactaac aataagtgtg ccagaattat ccgcgcggct gcaaaagact aatttgacgg
    40681 ataatgtgtg gggttgttca caaattgatg ccagcttctg gttgtttgta aaatacgaaa
    40741 acgtttggct ttttttatcg cgttatttat ttgctgctaa gtatcagaaa taagataaag
    40801 gggcaggcta tctctcttca gtttgataaa ctcaccgccg taattctcat attgatatag
    40861 aaatgtgata tctcttccat aattgcatta gaaaatgata aaaatcttat gtttttatca
    40921 aagccaattt cgcctgagtt aatatcatca tgatctgtga accatcaacg tcttcaatga
    40981 tcgactttta agtaagtctg gaaggtaaag ctatggagct tgctactacg cagtcagtat
    41041 tgatgcaaat tcaaccgaca attcagcgtt ttgccagaat gcttgccagc gttttgcagt
    41101 ttgaggttga gatcgttgat gaaaacttgt gtcgcgtcgc cggaacgggc gcgtatggga
    41161 agtttcttgg tcgccagttg agcggcaact cacgcctgct ccgccacgtc ctggaaacga
    41221 aaactgaaaa agttgtgaca cagtctcgct tcgatcccct ttgcgaaggt tgcgatagta
    41281 aagaaaattg ccgcgaaaaa gcatttctgg gtacgcctgt cattttacag gatcgttgtg
    41341 ttggggtgat aagtttgatt gccgttaccc acgagcaaca agagcatatc agtgataatt
    41401 tacgcgaatt ttctgattat gttcgccata tatccaccat ttttgtttcg aaacttctgg
    41461 aggatcaggg gccaggagat aacatcagta aaatattcgc gaccatgatc gataatatgg
    41521 atcagggcgt attagttgtt gatgatgaaa gtcgggttca gtttgttaat cagactgcct
    41581 taaaaacact tggtgttgta caaaataata ttattgggaa acctatccgt ttcagaccat
    41641 taacatttga gagtaatttt actcatggac atatgcagca tattgtttcg tgggacgata
    41701 aaagtgaatt aatcattggt caattgcata acattcaggg ccgacaatta tttttaatgg
    41761 catttcacca atcgcatacc agtttttctg tagcaaatgc acctgatgaa ccacatattg
    41821 aacaattggt tggcgagtgc cgtgttatgc ggcaattaaa acgactcatt agccgtattg
    41881 cacccagccc atccagcgtt atggtggttg gtgaaagcgg cacgggtaaa gaagtcgtcg
    41941 cccgagcaat ccataagttg agcggaagac ggaataaacc ctttattgct atcaactgtg
    42001 ccgcgattcc ggagcagctt ctggaaagcg aactgttcgg ttatgttaaa ggcgcattta
    42061 ctagtgcttc tgccaacggt aaaacagggt tgattcaggc ggcgaatacg ggcacgctgt
    42121 ttctcgatga aataggtgat atgccattaa tgttgcaggc taaattactg cgcgctattg
    42181 aggcgcgtga aattctgccg attggtgcca gtagcccaat acaagtcgac attcgcatca
    42241 tttctgcaac taatcagaat ttggcccagt tcattgccga aggtaaattc cgcgaagatc
    42301 tcttctaccg acttaatgtt atcccgataa ctctgccacc gctgcgtgaa cgtcaggaag
    42361 atattgaact attggtgcat tactttttac atctgcatac ccgtcgtctg ggatcggttt
    42421 atcctggcat tgctcccgat gtcgtcgaaa tattgcgtaa gcatcgttgg cccggaaacc
    42481 tgcgcgagtt aagcaatttg atggaatatc tggttaacgt ggttccttca ggtgaagtta
    42541 tcgacagcac gctattgccg ccaaatctgc tgaataatgg cacaacggag caaagtgatg
    42601 taacagaggt cagtgaggcg cacctgtcac tcgatgatgc gggcggcacg gcgctggagg
    42661 agatggaaaa gcaaatgatc cgcgaggcgc tttcacgtca taacagcaag aagcaagttg
    42721 ctgatgaact gggcatcggc attgctacgc tctatcgcaa gattaagaaa tatgagttgt
    42781 taaacacata acctcagtga atccggcctg aattcaggcc ggatatcctt ttacttcgtt
    42841 ttctcgcaat ccagaactgt atttacaatc atctgatacc ccgtgcagcg acaaagattt
    42901 cccgccagtc cgcgacgaat ttccgtaatg gttaatggct tctcgcgtgg tttcgccagc
    42961 attgccgtgg tagccataat caggccaggc gtacaaaacc cgcactgcac tgcgccggat
    43021 ttcgcataag cctgctgaac atgagaaagt tttccgcctt tcgcttcacc ttccagcgtg
    43081 cggatctcct ttccttcagc ccaggcggca aggtataagc aactgtctat tgctgtgccg
    43141 tcgaccaaca ccgtacaggc accacattca cccacgcagc acccttgttt gacacttagc
    43201 agtccttgtt cgcggagtaa ttccgagagc ggcgtgcctg gtgcggcgtg aagctgaaaa
    43261 ggcatcccgt taatggtgca ttcgatggta attgtttcgc tgtgattcat tgcaattttc
    43321 cccccgccgc ggcgacggct tcgctaatca cttttttggt catcgtctgg atgagatgca
    43381 gacgaaactc tttactggcc cgccatgaag aacgcggggc gacatcttgc aggacagatt
    43441 cgctgatagc ttccagcgtt tgcaggttta atggcgcatt ttgtgcagtc tgttcggcat
    43501 gttggcagcg aatcggcgtt ggcgcggcaa caccaaatgc caggcgtaat tcgctgaaat
    43561 tgccgttatc cagtcggcaa tgtgcggcgc agccaatcgt tgaaatatcc attgcgtcgc
    43621 gcatggcata tttaaaatgc gcgctgcccg cgtgttcttt cggctgtggc ggaaaatgaa
    43681 aggcgacgag gatttcgtca tgctcaagag acactttgcc cggcccggtg tgaaagccat
    43741 taatcgggac gaaacgaaca ccgcgcgggg agtggatctc cagtttcgcg tcataaatta
    43801 gcgttggcgt ggcagaatct gcgctggtgg caccgttgca aatatttcca ccgtaggtag
    43861 cgacgttacg gatctgcggt ccagcaatgg acgtggccgc agcacataac gccgggagat
    43921 gacgttgagt tataggagct tctattagct gggtaaatgt cgttgcagag ccgatacgta
    43981 gcgagccatc ttccgccagc gtaattcccc gcagctccgc cagattatga atatcaacaa
    44041 tatggcgata acggtcattg tgatggtgga gctgaatcag tacgtcagtg ccatcggcga
    44101 gcagtttggc ctgcgggttg tcagccagca ggttgatggc atcggcaagg gttgctgcgc
    44161 gatggtaaga agcaaaatca aacatgatgt tatcctcaaa tcaatcctgc cagatggaac
    44221 tcttcatata accgttttgg cgtcagcggc agtgtattga ttgcaacacc ggtagccatc
    44281 ttcaccgcgt tacgaatagc agcggcaaca ggaattattg gtggctcacc cagtgactta
    44341 tgtccgtatg cggattgcgg ctcattgatt tcgacgaacg cgctttccag ttgtggcaga
    44401 tccggcatgg tcggcatttt gtaatccagc agattggggt tacggaccac gccgctttta
    44461 gcatcgatga tcatctcttc aaatagcgcc cagccaatgc ccattcccat tccgccgtgt
    44521 acctgacctt ctgccagcag tggattaaga atatgccctg aatcatgaac gttgaggatg
    44581 cggttgatgg tgactttgca cagcgcaata tcgaccgtca gatcaacaaa ggtacagcca
    44641 aacgccgatg ggttagtggt ggttttgatg gagctttcag cagagagctg cccgccgcgt
    44701 tcagggtggt agaaagcgtc catcgccaaa tcttttaacg acattaacgg ctcttccggt
    44761 cgttcaacca gcacgatatg gccttttatc agggtcagat tcatcgctga ctgatgtagc
    44821 atgactgcgg cgtgagcgat gattttctct tttaataata gtgccgcact gcgcagcgca
    44881 ggcgcggcaa catagctctg gcgtgaggca aatgcgccgg gagcgaacgg cgtaacgtcg
    44941 gtatcttgag ttgaaataac gcgaacgtcg ctgaccggaa cccccacggt ttctgccacc
    45001 atttgcgaga agacggtgtc ggcaccctga ccgatttccg tcgcgccgct ttgcacgttg
    45061 atggttccat cctgattcat cagaaggcgc gcgcctgcta tttctacgcc gacaggccag
    45121 gtgttagagg tatagctaaa acaggcgacg ccaacgccgc ggcgcaaatt gccttgctgg
    45181 ttctggcatt ctgcacggcg tttttcccat tcaaagattt tccggccttt ttcaagacac
    45241 tccggcaacc ctgcgctgta aatacgtttg cccgtgagcg gattagcatc tccttcgcgg
    45301 gcggcgttgc gtaaacgaat ttcaacagga tcaataccta acgctgtcgc ggcgtcatca
    45361 agcatagact caacggcaaa tacgacttgt ggcgcgccat aaccacgcat cgcaccagcc
    45421 gaggggaggt tggtatagca ggtctttgaa ctgtaagcgt aggcacaacg aggataaagg
    45481 taagcgactt tattcctccc cgcagaagcg atggagtgcc cgtgagatgc ataagcgccg
    45541 gtgttagaca gaacatccag actataacct ttcaatgttc cgtcgcggtt cacgcccatt
    45601 tgcccgtcaa tggtaaaagc gtggcgggta cgggttgcga ggaaacactc ttcacggcta
    45661 agggaaactt tcaccggaat gccgccaagc ttgctggtca ggaatgctgc cattggctct
    45721 tccagtacat cctgtttatt accaaaaccg ccaccgacaa acggtttgat gactcgtacg
    45781 catgaccagg gaatatccag cgcctgacca accacgcggc gaacaatgtg cgggatctgg
    45841 gtgctggaaa cgatggtaat tcgcgagtca tcctccatcc acgccagcga tgttacgctt
    45901 tccatgtgac aatgttgaat aacgggggtc tgatagtgcc cctgtacctg gtagtcggcg
    45961 gcatcgattg tttgttggac attacccgtc gacatcgtgc tttgtttcag taaattgcca
    46021 ccgttatgga ttggtgcagc gtcttctgcc agcgccgctt ctggcgtggt gataacgggt
    46081 aattcttgcc actcaatgct gaccaattgc gccgcttttt ctgccgtgag ttcatcgcgg
    46141 gccacgacga tggcaacggc gtcaccatga tgacgaacat ggcgagttag cagtgcgcga
    46201 tcggcggtat cgcgcttgtt ttcgtcaagt gtccaggcat gcccagctgt agcgaatgga
    46261 atatcaggca catcttccca ggtaaaaatc gccagtacgc ccggcaaact tctggcttgt
    46321 tcatcattaa tacttacggc ataaccatgt gcgatagggc tacgtacata tttcgcataa
    46381 cacatgcccg ccataacata atcgtcagta tatcgtgccc gcccggtgac cttagcaatg
    46441 gcatcgacgc gcatgcatga ttcacccgta gcggttgctt cccgcgcttc catagaaatc
    46501 ccctcgattt gaacatttgg ttcttatggc aaagatttag tctaagcagg caagatttac
    46561 gccagaaacg acgataagcg ttaggggagg taggaagtgt gatgacgtta aaaaaacaca
    46621 gatatgatac gaaacaggac tctgaatagg ggcagtgata cgtatcaata tgagtggcga
    46681 taaaatcaat gtgataaatt gggattatta cgcatcagga tgggaaatag ggtaaaaagt
    46741 caggaagttc gatacctcta cagcgaatat tttgctgatg atacttccct ttgccaaatg
    46801 aaattaacct ctttctctat taccacgttt ttccagaagc aagagattgg gtatcaagag
    46861 gctggctgct atgataaggc gtcttgtttt ttaagcgagg aaagattttg agtgcgggac
    46921 gcctgaataa aaaatctctg ggtatcgtga tgttgttatc ggttggactg cttttggcgg
    46981 gctgttcggg tagcaaatca tccgatacag gaacgtattc cggctccgtt tacaccgtga
    47041 aacgggggga tacgctatat cgtatttcgc gcaccacggg aaccagcgta aaagaactgg
    47101 cgcgactgaa cggcatttcc cccccttaca ccattgaagt tggtcagaaa ctaaaactgg
    47161 gtggggcgaa aagtagcagt agtacacgta aatcaaccgc caaatcaacg accaaaaccg
    47221 catcggttac accgtcatca gcggtaccga aatcatcctg gccgccagta gggcaacgtt
    47281 gttggttatg gccaacgaca gggaaagtta tcatgccgta ttcgacagca gatggcggca
    47341 ataaagggat tgatatctca gctccacggg gtacacctat ttacgccgcg ggtgcaggaa
    47401 aggtggtgta tgtgggcaac cagctgcgtg gctacggtaa tctcatcatg attaaacaca
    47461 gtgaagatta cattacggct tacgcccata atgacacgat gctggtaaat aatgggcaaa
    47521 gcgtgaaggc tgggcaaaaa atcgccacta tggggagcac ggatgcggca tctgttcgcc
    47581 tgcatttcca gattcgttac cgtgcaacgg caattgatcc gctacgttac ttgccgccgc
    47641 agggcagcaa gccaaaatgc tgatggcgaa ttaatcagca gtcagcagcg cggcacttgc
    47701 taaggagagc gtaaggttta taatgcctta cgcatctcga agcgggcgta gttcaatggt
    47761 agaacgagag cttcccaagc tctatacgag ggttcgattc ccttcgccgc ttttgctgta
    47821 tctgattttc tatattatct taattcaatc gcttcagact tttcgcaatc gccgtaatat
    47881 ttccccattt tgttgtaaat aaaaagcgtt tacgcaatgc aatccttttg taacaaccca
    47941 ggtaaaacac cttattttca agttgttaaa taagtggtta tttacgcttt taacaaacga
    48001 ttgatggaat attttatgaa agggaaatct gcattaactc ttctgctcgc tggtatattt
    48061 tcgtgcggca catgccaggc gacaggggca gaagtgacga gtgaatctgt atttaacatt
    48121 cttaattcca cgggggcagc aacagataaa agttatttat cgttaaatcc agataagtac
    48181 cctaattatc gtcttctgat tcactcagcc aaattaaaaa atgaaataaa gagccactat
    48241 accaaagacg aaattcaagg gttgttaaca ttaacagaaa acacacgaaa gttaacgttg
    48301 acggagaaac cctggggaac atttattctt gcttctactt tcgaagatga caaaacagcg
    48361 gcagaaaccc actatgatgc tgtctggctg agggatagtt tatggggcta tatggcatta
    48421 gtatcagatc agggaaatag tgtagccgca aaaaaggttc tgctcaccct gtgggattat
    48481 atgtctacgc tcgaccagat taagcggatg caggacgtca ttagcaaccc caaacgactg
    48541 gatggagttc ccgggcaaat gaatgctgtg catatccgat ttgacagcaa ctcccccgtg
    48601 atggcagatg tgcaggaaga aggcaagccg cagctgtgga accataaaca aaatgatgcg
    48661 ttaggtcttt atcttgatct ccttatacaa gcaatcgaca ctggtacaat taacgctgaa
    48721 gattggcaaa aaggtgaccg attaaaatcg gttgcgctgc tgattgctta tctggataaa
    48781 gccaatttct atgttatgga agattccgga gcatgggaag aagatgcccg attaaacaca
    48841 tcttccgttg cactggtgac ctctggactg gaacgtttgt cgaacttact ctcgaagaaa
    48901 gactctgttt ttgtctctga tttactacgc gaagctaaag ccaatgaact ggatgaacct
    48961 ctttcaacaa cgaggttgaa ccacctcata gataaaggtt atgaaagaat aacgttacag
    49021 cttgatttag ggggagaatc cccaggttat ctggaaaaag ataaacatta cagagaagcc
    49081 gatgcggctt tacttaatgt tatttatcct gcaaatcttg caaagataaa caccagaaga
    49141 aaagagcagg ttctaaaaat agttaaaaaa ctagcaggtc cgtatggtat aaaaagatac
    49201 gagaaggata attatcagtc ggcaaatttt tggttcaatg atattaaaac cgataccgat
    49261 caaaattcac atgcgaaaag
//
LOCUS       DAZPLI010000013        47297 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZPLI010000013 DAZPLI010000000
VERSION     DAZPLI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47297
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.013"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..264)
                     /locus_tag="ACHUU3_000699"
     CDS             complement(<1..264)
                     /locus_tag="ACHUU3_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR2080862.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAP"
     gene            complement(424..636)
                     /gene="rpmE"
                     /locus_tag="ACHUU3_000700"
     CDS             complement(424..636)
                     /gene="rpmE"
                     /locus_tag="ACHUU3_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR2080863.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            839..3037
                     /gene="priA"
                     /locus_tag="ACHUU3_000701"
     CDS             839..3037
                     /gene="priA"
                     /locus_tag="ACHUU3_000701"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR2080864.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRHGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3193..4218
                     /gene="cytR"
                     /locus_tag="ACHUU3_000702"
     CDS             3193..4218
                     /gene="cytR"
                     /locus_tag="ACHUU3_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR2080865.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4310..5269
                     /gene="ftsN"
                     /locus_tag="ACHUU3_000703"
     CDS             4310..5269
                     /gene="ftsN"
                     /locus_tag="ACHUU3_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR2080866.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5362..5892
                     /gene="hslV"
                     /locus_tag="ACHUU3_000704"
     CDS             5362..5892
                     /gene="hslV"
                     /locus_tag="ACHUU3_000704"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR2080867.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5902..7233
                     /gene="hslU"
                     /locus_tag="ACHUU3_000705"
     CDS             5902..7233
                     /gene="hslU"
                     /locus_tag="ACHUU3_000705"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR2080868.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7300..8226
                     /gene="menA"
                     /locus_tag="ACHUU3_000706"
     CDS             7300..8226
                     /gene="menA"
                     /locus_tag="ACHUU3_000706"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR2080869.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8319..8804
                     /gene="rraA"
                     /locus_tag="ACHUU3_000707"
     CDS             8319..8804
                     /gene="rraA"
                     /locus_tag="ACHUU3_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR2080870.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8889..9134)
                     /gene="zapB"
                     /locus_tag="ACHUU3_000708"
     CDS             complement(8889..9134)
                     /gene="zapB"
                     /locus_tag="ACHUU3_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR2080871.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9560..10405
                     /gene="glpF"
                     /locus_tag="ACHUU3_000709"
     CDS             9560..10405
                     /gene="glpF"
                     /locus_tag="ACHUU3_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFR2080872.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10428..11939
                     /gene="glpK"
                     /locus_tag="ACHUU3_000710"
     CDS             10428..11939
                     /gene="glpK"
                     /locus_tag="ACHUU3_000710"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR2080873.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            12069..13079
                     /gene="glpX"
                     /locus_tag="ACHUU3_000711"
     CDS             12069..13079
                     /gene="glpX"
                     /locus_tag="ACHUU3_000711"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR2080874.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13176..13922
                     /gene="fpr"
                     /locus_tag="ACHUU3_000712"
     CDS             13176..13922
                     /gene="fpr"
                     /locus_tag="ACHUU3_000712"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR2080875.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13927..14355)
                     /gene="uspD"
                     /locus_tag="ACHUU3_000713"
     CDS             complement(13927..14355)
                     /gene="uspD"
                     /locus_tag="ACHUU3_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR2080876.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINQMSADLLIVPFIDK"
     gene            complement(14382..14681)
                     /locus_tag="ACHUU3_000714"
     CDS             complement(14382..14681)
                     /locus_tag="ACHUU3_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR2080877.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14893..15333)
                     /locus_tag="ACHUU3_000715"
     CDS             complement(14893..15333)
                     /locus_tag="ACHUU3_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR2080878.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15434..16033
                     /locus_tag="ACHUU3_000716"
     CDS             15434..16033
                     /locus_tag="ACHUU3_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR2080879.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16141..16908
                     /gene="tpiA"
                     /locus_tag="ACHUU3_000717"
     CDS             16141..16908
                     /gene="tpiA"
                     /locus_tag="ACHUU3_000717"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR2080880.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16963..17718)
                     /gene="cdh"
                     /locus_tag="ACHUU3_000718"
     CDS             complement(16963..17718)
                     /gene="cdh"
                     /locus_tag="ACHUU3_000718"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR2080881.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17825..18814)
                     /gene="sbp"
                     /locus_tag="ACHUU3_000719"
     CDS             complement(17825..18814)
                     /gene="sbp"
                     /locus_tag="ACHUU3_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR2080882.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19134..20096)
                     /gene="pfkA"
                     /locus_tag="ACHUU3_000720"
     CDS             complement(19134..20096)
                     /gene="pfkA"
                     /locus_tag="ACHUU3_000720"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR2080883.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20277..21179)
                     /gene="fieF"
                     /locus_tag="ACHUU3_000721"
     CDS             complement(20277..21179)
                     /gene="fieF"
                     /locus_tag="ACHUU3_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR2080884.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21414..21632)
                     /gene="ogrK"
                     /locus_tag="ACHUU3_000722"
     CDS             complement(21414..21632)
                     /gene="ogrK"
                     /locus_tag="ACHUU3_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFR2080885.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(21714..22877)
                     /locus_tag="ACHUU3_000723"
     CDS             complement(21714..22877)
                     /locus_tag="ACHUU3_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFR2080886.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDITLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQITITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHCLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(22877..23356)
                     /locus_tag="ACHUU3_000724"
     CDS             complement(22877..23356)
                     /locus_tag="ACHUU3_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2080887.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISKVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            complement(23371..25818)
                     /locus_tag="ACHUU3_000725"
     CDS             complement(23371..25818)
                     /locus_tag="ACHUU3_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR2080888.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRRASKLLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTIAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWVQKNKSLASTIGLIAGGALALTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(25811..25966)
                     /locus_tag="ACHUU3_000726"
     CDS             complement(25811..25966)
                     /locus_tag="ACHUU3_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFR2080889.1"
                     /translation="MTLPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGNTNE"
     gene            complement(25963..26238)
                     /locus_tag="ACHUU3_000727"
     CDS             complement(25963..26238)
                     /locus_tag="ACHUU3_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR2080890.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPSAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(26295..26813)
                     /locus_tag="ACHUU3_000728"
     CDS             complement(26295..26813)
                     /locus_tag="ACHUU3_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFR2080891.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(26826..28016)
                     /locus_tag="ACHUU3_000729"
     CDS             complement(26826..28016)
                     /locus_tag="ACHUU3_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFR2080892.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTVSEVKAYRQNFSQRELMVIWPDFLAWDTVASTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            28427..29845
                     /locus_tag="ACHUU3_000730"
     CDS             28427..29845
                     /locus_tag="ACHUU3_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2080893.1"
                     /translation="MTYALIDNSTLTAVQRIEGKVKTRSKDSVDTDIIAFENYIQSIL
                     FYDHVIAVDDYIPEYRGSRISSFREILFLDKAQYGLDEIESEAKKISDALKPKIRGGT
                     FVNDDFKKLIELLQTHIICTWDISSSVYHLTLKNLSDGGKEFEKYGNIAAAIFSELRD
                     AAESGNRTNGDVELLDRFGNPIRKGYKVPGAKWGDGTSAGEASDAIKAFVASLVWLAN
                     RAVFYSLTAKYLQADTFLYPLRQAYQQTYLSQNCQYGFDYAKHIVENFSTSLSHDLID
                     IHSGGKAVATATSLPLFSAWLAKETGDPSCIVEAAYNIRDNNEFVRAREKMREIKRLF
                     DENELPQANKSVRALLTEVSKESDYIRTKYGLKTRQGVPVTKLVHVYNTVAAINSLPK
                     LPTFNFKVKVPDFLYDLKKPKGFRALYRNLTNDLSTVWSLGEARDILGSKVVIDNHAR
                     IYSPKHEEPRFKNSHSPYKSPM"
     gene            complement(29960..30487)
                     /locus_tag="ACHUU3_000731"
     CDS             complement(29960..30487)
                     /locus_tag="ACHUU3_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR2080894.1"
                     /translation="MQHLINITAGNPKTVEQYQLTKDFDVVWFFSEDGKNWYEEQKYF
                     ADDTIKIAYDKDNIIRYVEKDVTAIRPDGLSVVEVPDITANRRADISGNWMFKDCTVI
                     KRIYTAEELQQQAENRKARLLADAESVILPLERAVRLNMATDEERSRLEAWERYSVLV
                     SRVDPANPEWPEMPQ"
     gene            complement(30491..32809)
                     /locus_tag="ACHUU3_000732"
     CDS             complement(30491..32809)
                     /locus_tag="ACHUU3_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR2080895.1"
                     /translation="MSTKFRTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHTLNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVELTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARK
                     GLVQLSSATNSTSETLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSATNSDSE
                     TLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSAINSDSETLAATPKAVKSAYD
                     NAEKRLQKDQNGADIPGKDTFTKNIGACRAYSGALSTEAGNWTTAQFIEWLDSRGAFN
                     HPYWMCKGSWSYANNKIITDTGCGDIHLAGCVVEVMGTKSAITIRVTTPTTSSGGGTT
                     SAQFTYINHGDGYSPGWRRDWNRQGDSMTGTINQDGGSQNAYMSTALCSGTRGGKKYL
                     RKFRGGEGDTIWHETVQGGVIRWATGNNDAQEELSLSSAYGLRSRGEITSLSANGLRI
                     AYGNYGFFIRNDGGSTYLMLTASGDKFGTWNGLRPLTINNANGGVSMGHGLSVTGDIV
                     SSTKVRAGSGKKFTVSSSNTSTKEAAFNLWGNSSRPVVAELGDDAGWHFYSQRNTDNS
                     ITFAVNGQVSPSNYGNFDSRYVRDIRLGGAATYKPANNGMTWTHQAPSGCVYSGIIVQ
                     DTGSNSADNIGGVYYRPVQKYINGTWYNVAQV"
     gene            complement(32820..33350)
                     /locus_tag="ACHUU3_000733"
     CDS             complement(32820..33350)
                     /locus_tag="ACHUU3_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFR2080896.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVRIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWENSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            complement(33343..34251)
                     /locus_tag="ACHUU3_000734"
     CDS             complement(33343..34251)
                     /locus_tag="ACHUU3_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFR2080897.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLI
                     ITPADDSTLPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELTAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            complement(34256..34603)
                     /locus_tag="ACHUU3_000735"
     CDS             complement(34256..34603)
                     /locus_tag="ACHUU3_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFR2080898.1"
                     /translation="MTARYLGMNRSDGLTVSDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEARVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(34600..35235)
                     /locus_tag="ACHUU3_000736"
     CDS             complement(34600..35235)
                     /locus_tag="ACHUU3_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR2080899.1"
                     /translation="MNTLANIQELARALRNMIRTGIIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVTVKASTRVTLDTPEVVCT
                     NKLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(35302..35754)
                     /locus_tag="ACHUU3_000737"
     CDS             complement(35302..35754)
                     /locus_tag="ACHUU3_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFR2080900.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEETRKDGKKIHYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(35747..36214)
                     /locus_tag="ACHUU3_000738"
     CDS             complement(35747..36214)
                     /locus_tag="ACHUU3_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2080901.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(36177..36422)
                     /gene="lysC"
                     /locus_tag="ACHUU3_000739"
     CDS             complement(36177..36422)
                     /gene="lysC"
                     /locus_tag="ACHUU3_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFR2080902.1"
                     /translation="MLCAGCTSAQPAQTPVIVHNACPKVSLCPMPGSDPQTNGDLSAD
                     IRQLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            complement(36322..36747)
                     /gene="lysB"
                     /locus_tag="ACHUU3_000740"
     CDS             complement(36322..36747)
                     /gene="lysB"
                     /locus_tag="ACHUU3_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFR2080903.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLVKHKNASLRALLDRANNVASEQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRTACADASDCPQRLPESEPLPDAGQ"
     gene            complement(36735..37160)
                     /locus_tag="ACHUU3_000741"
     CDS             complement(36735..37160)
                     /locus_tag="ACHUU3_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFR2080904.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VIAGGVGVYAAEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALMVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRTACRQRLEGREPCQN"
     gene            complement(37175..37672)
                     /locus_tag="ACHUU3_000742"
     CDS             complement(37175..37672)
                     /locus_tag="ACHUU3_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFR2080905.1"
                     /translation="MPVISTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(37672..37953)
                     /locus_tag="ACHUU3_000743"
     CDS             complement(37672..37953)
                     /locus_tag="ACHUU3_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR2080906.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            complement(37957..38160)
                     /locus_tag="ACHUU3_000744"
     CDS             complement(37957..38160)
                     /locus_tag="ACHUU3_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFR2080907.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVEAVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(38160..38669)
                     /locus_tag="ACHUU3_000745"
     CDS             complement(38160..38669)
                     /locus_tag="ACHUU3_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFR2080908.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFSRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(38769..39512)
                     /locus_tag="ACHUU3_000746"
     CDS             complement(38769..39512)
                     /locus_tag="ACHUU3_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFR2080909.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDPRLLTDTLELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALVHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRTPRKTRSVTPAKRGRPKKKAS"
     gene            complement(39516..40588)
                     /locus_tag="ACHUU3_000747"
                     /pseudo
     CDS             complement(39516..40588)
                     /locus_tag="ACHUU3_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
     gene            complement(40647..41501)
                     /locus_tag="ACHUU3_000748"
     CDS             complement(40647..41501)
                     /locus_tag="ACHUU3_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFR2080910.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            41675..43447
                     /locus_tag="ACHUU3_000749"
     CDS             41675..43447
                     /locus_tag="ACHUU3_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFR2080911.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKVSVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            43447..44481
                     /locus_tag="ACHUU3_000750"
     CDS             43447..44481
                     /locus_tag="ACHUU3_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR2080912.1"
                     /translation="MSKKKGKTPRPAAKTITASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            44853..45995
                     /locus_tag="ACHUU3_000751"
     CDS             44853..45995
                     /locus_tag="ACHUU3_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFR2080913.1"
                     /translation="MDIIDLKSQLENEDNAKKRAELLAQLEVIKQNAEEQIRAEQKDV
                     DFETKEFTVELLVNKYHNGLEDDTNELFVPDYQRDFVWSEKRQSKLIESLILGFPIPY
                     IFIADVLSEDPDLDGRIEIVDGSQRVRTIHAFIQNELVLQDLKSLDVLNGFKFQDLPL
                     SRQRRFMRIPVRVIELSSKCNEETRRDLFERINSGSDILKDMEVRKGSELGSTNLYTQ
                     VIKPCSTIPKFKELAPLSQTKEKRDERLEFTLRFFAYVDKYKNFDHSVRDFLNDYMSE
                     NRDIDETKQNSMKQEFANVLSFVENYFPAGFKKTITAKSTPRVRFESLAVGAALALRE
                     VPDLVPKNLDWLSSEEFKRLTTSDGANSRVKVIERIEYVRDKLLED"
     gene            45997..46677
                     /locus_tag="ACHUU3_000752"
     CDS             45997..46677
                     /locus_tag="ACHUU3_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAE_28990/MAE_18760 family HEPN-like nuclease"
                     /protein_id="HFR2080914.1"
                     /translation="MDRFREDFDERSGEILAYLDLLKFIEYAGAELISADDKEHKFSI
                     TAQSRKTLKGAVYILLYNLIESTMREAICLIHETIYDRNVEFDKLRKNLRSEILKRLK
                     NESVNVESLVNHLTKGISCGISYGTFNKKKIFSGNIDREEIKEKSKIYGFSTHSDYTH
                     TKHGEKLATVKQHRNDLSHGNVSFAEIGKNVSYQDLENISLEVIAYLDAIVNNIEHYI
                     NNNEYLEQ"
     gene            complement(46707..>47297)
                     /locus_tag="ACHUU3_000753"
     CDS             complement(46707..>47297)
                     /locus_tag="ACHUU3_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFR2080915.1"
                     /translation="ASKIDCVEYGIPQNRSRHVLLASKLGAIELVKRDDVILKTVRDA
                     IGNLPPLEDGQTDPNDILHRASKLNPINKKRIIHSVPGGTWKDWPKELVAACHMKSSG
                     KGYASVYGRMSWDKPSPTITTLCYGFGNGRFGHPEQHRAISLREAALLQTFPMDYIFV
                     EDKNKFVIRSIGKMIGNAVPVELGKAIGQSIKNHLE"
BASE COUNT        11244 a        12623 c        12047 g        11383 t
ORIGIN      
        1 cggcgcgggc gtttttgtcc ggtaactgct gtaggtgcgg gagaggatga acggcagcgg
       61 ggcgggcagg gcgatgtcgg tttcaccggg aaggaccttc gcaccgagca gcggattgac
      121 cggatggccg gacgtcaccc cgccggggca caccgaacag gccacgccgg tgggggcacc
      181 gatgcgcacc ccggctgaac cctggacaat gctaccgcca tactgcgtca tgtcgccctg
      241 acgcgccgcc ggttttccgc tcatcgtaaa atccttttga aataaataag aaccagaagc
      301 ctagcgccag tcaacaacag gaaatgataa tgagttaaca ccccggaatt tttcagaatt
      361 tataaaaggc aaattacagg cacaaaaaaa gcgccgtgcg gcgctttttt cggaaatccg
      421 gtcttatttg ctgcccggga tgttgaaacg cttgttgaag cggtcaacac ggccaccggt
      481 agcaacatca cgctgtttgc cagtgaagaa cgggtggcac ttgctgcaca cgtcgaggtt
      541 caggtcatga ccaacggtgg agcggatttt cattacgtta ccgcaagagc agctagcagt
      601 aatttcttcg tatttcgggt gaatatcttt tttcatggga aaacctcggt ttaaggccgc
      661 gtcgctcttc cagccctaac gccagacacc acgcgatgtt aaaagtatag cttcaatacg
      721 atcatttcgt acgaaggcgc gaaatcatac agaaattaac cagcgtatgc aaactgatcc
      781 gcactcttct acggcaatgt gtatactaac ccaccgaatt tcaagtcagg atgatgctat
      841 gcccgttgcc cacgttgcct tgcccgttcc gcttcctcgt acctttgact atctgctgcc
      901 agaaggcatg gcggttaaag ccgggtgtcg cgtgcgcgtg ccgtttggca aacagcagga
      961 gcgcatcggg gttgtggtat cagttagcga tgtcagtgag ctgccgctta acgagctgaa
     1021 agcggtagtt gaagtgctgg acgttgagcc ggtatttact cactccgtct ggcgattact
     1081 gctctgggcg gcagattact atcatcatcc gattggcgat gtgctgtttc atgccttgcc
     1141 gattttactg cgccatgggc ggcctgcggc gaacgcgccg atgtggtact ggtttgccac
     1201 tgaacaaggc caggcggtgg atctgaacag cctgaaacgc tcacccaagc aacaacaggc
     1261 gctggcggcg ttacggcaag ggaaaatctg gcgcgaccag gttgccacgc tcgaatttaa
     1321 tgatgccgcg ttgcagacgc tgcgcaaaaa aggtctgtgt gatttagcaa gtgaaacacc
     1381 agagtttagc gactggcgaa cgaactatgc cgtttctggt gagcggttgc gattgaatac
     1441 cgaacaggcc accgccgttg gcgcaattca tagcgcggca gatacttttt ctgcctggct
     1501 gctggcgggc gttaccggtt ccggtaaaac ggaggtttat ctcagtgtac tggaaaacgt
     1561 gctcgctcag ggcaaacagg cgctggtgat ggtgccggaa atcggcctga caccgcaaac
     1621 tatcgcccgt tttcgtgaac ggtttaatgc gcccgtggaa gttctgcatt ccggtctgaa
     1681 cgacagcgag cgtctttcgg cgtggctgaa agcgaaaaat ggcgaggcgg cgattgtgat
     1741 cggcacccgc tccgcgctgt ttacgccgtt taaaaatctc ggcgtgattg tcattgacga
     1801 agagcacgac agttcctaca agcagcagga aggctggcgc tatcatgccc gcgacctggc
     1861 ggtgtatcgt gcgcatagcg agcaaatccc gattattctt ggctccgcaa cgcccgcgct
     1921 ggaaacgttg tgcaacgtcc agcagaaaaa ataccgcctg ctgcgcctga cgcgccgggc
     1981 ggggaatgcg cgtccggcaa ttcaacacgt gctggattta aaaggtcaga aggtgcaggc
     2041 aggtctggct ccggcgttaa tcacccgtat gcgccagcat ttacaggcca acaatcaggt
     2101 tatcctcttt cttaaccgcc gtggctttgc gcctgcgcta ctgtgccacg actgcggctg
     2161 gattgccgaa tgcccacgtt gcgatcacta ctacacgctg catcaggcgc aacagcattt
     2221 gcgctgtcac cactgtgaca gtcagcgtcc ggtgccgcgc cagtgccctt cctgcggttc
     2281 cacgcacctg gtacccgtgg ggctgggcac cgaacagctt gaacagacgc ttgcgccgct
     2341 gttccctgat gtgcccattt ctcgtatcga ccgcgatacc accagccgca aaggggcgct
     2401 ggaacagcaa ctggcggaag tacatcgcgg cggtgcgagg attttgatcg gcacacaaat
     2461 gctggcgaaa ggtcaccact ttccggatgt gacgctggtg gcgttactgg atgtggacgg
     2521 cgcgctgttt tctgccgatt ttcgctcggc agagcgtttc gctcagcttt acacccaggt
     2581 cgccggtcgt gccgggcgtg cgggtaaaca gggcgaagtg gtgctgcaaa cgcaccatcc
     2641 ggaacatcct ctgttgcaaa cgttgctcta taaaggctac gacgcctttg ccgaacaggc
     2701 gctggctgag cgacgcatga tgcagctgcc gccgtggacc agccatgtga ttgtgcgtgc
     2761 ggaagatcat aacaatcagc atgcgccgct gttcctgcaa caactgcgta atctgatcct
     2821 ctccagcccg ctggcagacg acaaattgtg ggttctcggt ccggttccgg cactggcacc
     2881 taaacgtggc ggtcgctggc gctggcagat attgttgcag cacccttccc gcgtgcgctt
     2941 gcaacacatc attaacggta cgctggcact catcaataca ataccggatt cccgtaaggt
     3001 gaaatgggtg ctggatgttg atccgattga gggttaaacc gctcacgatg cgaggcggat
     3061 cgaaaaattc aatattcatc acacttttca tgaaaattct gtaaccgttt tcacgcgcta
     3121 tctgctaaaa atgttgccga tgtgaagtaa acatggatgt agtacgcctg acgtgccagg
     3181 cgaggagtga gtgtgaaagc gaagaagcag gaaactgccg cgaccatgaa agacgttgcc
     3241 ctcaaggcaa aagtctctac agcgactgtc tcccgagcat taatgaatcc cgataaagtc
     3301 tcccaggcca cccgtaatcg ggttgaaaaa gcggcccggg aagtgggtta tttaccgcag
     3361 cctatggggc gcaacgtcaa gcgtaatgaa tcccgcacca ttctggtgat tgtcccggat
     3421 atctgcgatc ccttctttag cgaaattatt cgcggtatcg aagttacggc ggcaaatcac
     3481 ggatatctgg tgctgattgg cgactgtgcg catcaaaatc agcaggaaaa aacctttatc
     3541 gatttgatca tcaccaagca aattgatggc atgttgctgc tgggttcaag gctgccgttt
     3601 gatgccagca ttgaggaaca gcgtaatctg ccgccgatgg tgatggcgaa cgaattcgca
     3661 ccggaactgg agctacctac agttcatatc gacaatctga ccgccgcatt tgatgcagta
     3721 aattatctat atgagcaagg gcataaacgg attggctgta tagccggtcc cgaagagatg
     3781 ccgctgtgtc actaccgcct gcaaggttac gttcaggcgc tgcgtcgctg cggcattatg
     3841 gttgatccgc aatacatcgc ccgtggcgac ttcaccttcg aagccggaag caaagcgatg
     3901 cagcagctgc ttgatcttcc acaaccgcct actgctgtct tctgccatag cgatgtgatg
     3961 gcgctcggcg cactttctca ggcaaaacgc caggggctga aagtcccgga agacctttcc
     4021 ataatcggtt ttgataacat cgacctgacg caattttgtg atccgccgct gacaaccatc
     4081 gcgcagccgc gttacgaaat cggtcgggaa gctatgctgt tattgcttga tcaaatgcag
     4141 gggcaacacg ttggcagtgg ctctcgttta atggactgcg aacttatcat ccggggatca
     4201 acacgcgcgt taccttaaag taaaccttta agactgccgt gactggtcaa aggcccgccg
     4261 cttaagtaac atggcgggct gacgaacgaa taaatacagc gaaacgatag tggcacaacg
     4321 agattatgta cgccgcagcc aaccggcacc ttcgcggcga aaaaagagca cctcacggaa
     4381 aaagcaacga aatctgcctg cggtttctcc cgctatggtc gctattgctg ccgccgtgct
     4441 tgtgaccttt atcggtggtc tgtacttcat tacgcatcac aagaaagaag agtccgagac
     4501 gctgcaaagc cagaaagtga ccggtaacgg actaccacca aaaccagaag aacgctggcg
     4561 ctacattaaa gagctggaaa gtcgccagcc gggtgtgcgt gcgcccacag aaccttctgc
     4621 cggtggtgaa gtgaaaacgc cggagcaact gacaccagaa caacgtcagc ttcttgaaca
     4681 aatgcaggct gatatgcgcc agcagccaac gcagctggtt gaagttccgt ggaatgaaca
     4741 gacgccggaa cagcgtcagc aaacgctaca acgccagcgt caggcgcagc aattagcgga
     4801 gcagcaacgc ctggctcagc agtcgcgtac gactgaacaa agctggcagc agcagacgcg
     4861 tacgtcgcaa gccgcgccgg tacaggcgca accgcgccag tctaaaccgg cttctactca
     4921 gcagccgtac caggatctgc tgcaaacccc tgcgcacacg accgcgcagt cgaagccgca
     4981 gcaagctgcg ccggttgctc gtgccgctga cgcgccaaaa ccgacggcgg agaaaaaaga
     5041 cgaacgccgc tggatggtgc agtgcggttc gttcagaggc gcggaacagg cagagacggt
     5101 acgtgctcag ctggcgttcg aaggatttga ctcgaaaatc accaccaaca atggctggaa
     5161 tcgtgtggtc attggcccgg tgaaaggcaa agagaacgca gacagcaccc tcaatcggtt
     5221 gaagatggcg ggtcatacaa actgcattcg gctcgccgcc gggggttgaa accctcaaaa
     5281 tcccccccat ctataattgc attatgcccc gtacttttgt acggggtttg tactctgtat
     5341 tcgtaaccaa ggggtcagct cgtgacaact atagtaagcg tacgccgtaa cggccatgtg
     5401 gtcatcgctg gtgatggtca ggccacgttg ggcaataccg taatgaaagg caacgtgaaa
     5461 aaggtccgcc gtctgtacaa cgacaaagtc atcgcgggct ttgcgggcgg tactgcggat
     5521 gcttttacgc tgttcgaact gtttgaacgt aaactggaaa tgcatcaggg ccatctggtc
     5581 aaagccgccg ttgagctggc aaaagactgg cgtaccgatc gcatgctgcg caaacttgaa
     5641 gcactgctgg cagtcgcgga tgaaactgca tcgcttatca tcaccggtaa cggtgacgtg
     5701 gtgcagccag aaaacgatct tattgctatc ggctccggcg gcccttacgc ccaggctgcg
     5761 gcgcgcgcgc tgttagaaaa cactgaactt agcgcccgtg aaattgctga aaaggcgttg
     5821 gatattgcag gcgacatttg catctatacc aaccatttcc acaccatcga agaattaagc
     5881 tacaaagcgt aaggatctcc catgtctgaa atgaccccac gcgaaatcgt cagcgaactg
     5941 gataagcaca tcatcggcca ggacaacgcc aagcgttctg tggcgattgc tctgcgtaac
     6001 cgctggcgtc gcatgcagct caacgaagag ctgcgccatg aagtgacccc gaaaaatatc
     6061 ctgatgatcg gcccgaccgg tgtcggtaaa actgaaatcg cccgtcgtct ggctaagctg
     6121 gcgaatgcgc cgttcatcaa agttgaagcg accaaattca ccgaagtggg ctatgtcggt
     6181 aaggaagtgg attctattat tcgcgatctg accgatgccg ccgtgaaaat ggtacgcgtc
     6241 caggctatcg agaaaaaccg ttatcgcgct gaagaactgg cagaagaacg tattctcgac
     6301 gtgctgatcc cacctgctaa aaacaactgg ggacagaccg aacagcagca ggaaccgtcc
     6361 gctgctcgtc aggcattccg caaaaaactg cgtgaaggcc agcttgatga caaagaaatc
     6421 gagatcgatc ttgccgcagc accgatgggc gttgaaatta tggctcctcc aggcatggaa
     6481 gagatgacca gccagctgca gtccatgttc cagaacctgg gcggccagaa gcaaaaagcg
     6541 cgtaagctga aaatcaaaga cgccatgaag ctgctgattg aagaagaagc ggcgaaactg
     6601 gtgaacccgg aagagctgaa gcaagacgct atcgacgctg ttgagcagca cgggatcgtg
     6661 tttatcgacg aaatcgacaa aatctgtaag cgcggcgagt cttccggtcc ggatgtttct
     6721 cgtgaaggcg ttcagcgtga cctgctgccg ctggtagaag gttgcaccgt ttccaccaaa
     6781 cacgggatgg tcaaaactga ccacattctg tttatcgctt ctggcgcgtt ccagattgcg
     6841 aaaccgtctg acctgatccc ggaactgcaa ggtcggctgc caatccgcgt tgaactgcag
     6901 gcactgacca ccagcgactt cgagcgtatt ctgaccgagc cgaatgcctc tatcaccgtg
     6961 cagtacaaag cactgatggc gactgaaggc gtaaatatcg agtttaccga ctccggtatt
     7021 aagcgcatcg cggaagcggc atggcaggtg aacgaatcta ccgaaaacat cggtgctcgt
     7081 cgtttacaca ctgttctgga gcgtttaatg gaagagattt cctacgacgc cagcgattta
     7141 agcggtcaaa atatcactat tgacgcagat tatgtgagca aacatctgga tgcgttggtg
     7201 gcagatgaag atctgagccg ttttatccta taatcgcgtt caatcatttt catcattgtt
     7261 tgatggggct gaaaggcccc atttttattg gcgcgtatta tgactgaaca acaaattagc
     7321 cgaactcagg cgtggctgga aagtttacga cctaaaaccc tccccctcgc ctttgctgca
     7381 attatcgtcg ggacggcgct ggcatggtgg caaggtcact tcgatccgct ggtcgccctg
     7441 ctggtactaa ttaccgccgg gctattacag atcctttcta acctcgccaa tgattacggc
     7501 gatgcggtaa aaggcagcga taaacctgac cgcattgggc cgctacgcgg catgcaaaaa
     7561 ggggtcatta cccagcaaga gatgaaacgg gcgctcatta ttaccgtcgt gctcatctgt
     7621 ctctccgggc tggcactggt tgcagtggca tgccatacgc tggccgattt tgtcggtttc
     7681 ctgattcttg gcgggttgtc gatcattgcc gctatcacct acaccgtggg caatcgtcct
     7741 tatggttata tcggtctggg tgatatttcc gtactggttt tctttggctg gttgagcgtc
     7801 atggggagct ggtatttaca ggctcataca ttgattccgg cactgatcct tccggcgacc
     7861 gcctgtggcc tgctggcaac ggcagtactg aacattaata acctgcgtga tatcaatagc
     7921 gaccgcgaaa atggcaaaaa cacgctggtg gtgcgcttag gtgaagtgaa cgcgcgtcgt
     7981 tatcatgcct gcctgctgat gggctctctg gtatgtctgg cgctgtttaa tctcttttcg
     8041 ctgcatagcc tgtggggctg gctgttcctg ctggcggcac cattactggt gaagcaagcc
     8101 cgttatgtga tgcgggaaat ggacccggtg gcgatgcgac caatgctgga acgtactgtc
     8161 aagggagcgt tactgactaa cctgctgttt gttttaggga tattcctaag ccagtgggca
     8221 gcataactga caaatatcaa ttaacaattg atgattttgc caacagccta catagcgcga
     8281 tatactgaaa attctcgcag caactgaatg ttaagcctat gaaatacgat acttccgagc
     8341 tttgtgacat ctatcaagaa gatgttaacg tcgtggaacc gctgttctcc aactttggcg
     8401 gacgggcgtc gtttggcgga caaataatca cggtaaaatg tttcgaggac aacgggttgc
     8461 tgtacgatct gctcgaacag aatggccgtg gtcgtgttct tgtcgtcgat ggcggtggtt
     8521 ctgttcgtcg cgcactggtc gatgctgaac tggcgcgtct ggcagtacaa aatgaatggg
     8581 aaggtctggt tatttacggc gcggtgcgtc aggtagatga cctagaagag ttggatatcg
     8641 gcatccaggc gatggcggca attccggttg gtgccgctgg cgaaggcatt ggcgaaagcg
     8701 atgtccgcgt caattttggc ggtgtaacct tcttctccgg cgaccatctt tatgccgaca
     8761 ataccgggat tattctttca gaagatccgc tggatattga atgataagaa aggcaccgca
     8821 aggtgccttt tttctgcgtt acctgttggc ctacacagta aagaaattac gcgaaagatg
     8881 aagcgtaatc agacctcttc catgcgaccc agcagggcct gcagacgttc ctgccagccg
     8941 ttctgctgtt ctttcagatg gttgttctca cgctccagct cttcgcgctg atgctgggca
     9001 ttttgaactt cctgcgacag tgagttgttt ttttctttca gctcttcgat ttccatctgc
     9061 aacagagtga tggtatcaat cgcctgctgt acttttgctt ccagtttctc aaacacttct
     9121 aatgacattg tcatacctct cctgaattgc aaggcgttga tggataaaaa atcctcgtcc
     9181 cgattaccgg tgacgcctta ataaatacga gcgcacttta gttagctccg attgtatgaa
     9241 gccgcgccat cgctgtccag cggcacgcct tgcagattac ggtttgccac acttttcatc
     9301 cttctcctgg tgacataatc cacatcaatc gaaaatgtta ataaatttgt tgcgcgaatg
     9361 atctaacaaa catgcatcat gtacaatcag atggaataaa ttgcgcgata acgctcattt
     9421 tatgacgaag cacacacatt ttaagttcga tatttctcgt ttttgctcgt taacgataag
     9481 tttacagcat gcctacaagc atcgtggagg tccgtgactt tcacgcatac aacaaacatt
     9541 aactcttcag gatccgatta tgagtcaaac atcaaccttg aaaggccagt gcattgctga
     9601 attcctcggt accgggttgt tgattttttt cggtgtgggt tgcgttgcag cactaaaagt
     9661 cgctggtgcg tcttttggtc agtgggaaat cagtgtcatt tggggactgg gggtggcaat
     9721 ggccatctac ctgaccgcag gggtttccgg cgcgcatctt aatcccgctg ttaccattgc
     9781 attgtggctg tttgcctgtt tcgacaagcg caaagttatt ccttttatcg tttcacaagt
     9841 tgccggcgct ttctgtgctg cggctttagt ttacgggctt tactacaatt tatttttcga
     9901 cttcgagcag actcatcaca ttgttcgcgg cagtgttgaa agtgttgatc tggcaggcac
     9961 tttctctact taccctaatc ctcatatcaa ttttgtgcag gctttcgcag ttgagatggt
    10021 gattaccgct attctgatgg ggctgatcct ggcgttaacg gacgatggca acggtgtacc
    10081 acgcggccct ttggctccct tgctgattgg tctactgatt gcggtcattg gcgcatctat
    10141 gggcccattg acgggttttg ccatgaaccc agcgcgtgac ttcggtccga aagtctttgc
    10201 ctggctggcg ggctggggca atgtcgcctt taccggcggc agagacattc cttacttcct
    10261 ggtgccgctt tttggcccta tcgttggcgc gattgtaggt gcatttgcct accgcaaact
    10321 gattggtcgc catttgcctt gcgatatctg tgttgtggaa gaaaaggaga ccacaactcc
    10381 ttcagaacaa aaagcttcgc tgtaatatga ctacgggaca attaaacatg actgaaaaaa
    10441 aatatatcgt tgcgctcgac cagggcacca ccagctcccg cgcggtcgta atggatcacg
    10501 atgccaatat cattagcgtg tcgcagcgcg aatttgagca aatctaccca aaaccaggct
    10561 gggtagaaca cgacccaatg gaaatctggg ccacccaaag ctccacgctg gtagaagtgc
    10621 tggcgaaagc cgatatcagt tccgatcaaa ttgcagctat cggtattacg aaccagcgtg
    10681 aaaccactat tgtctgggaa aaagaaaccg gcaagcctat ctataacgcc attgtctggc
    10741 agtgccgtcg taccgcagaa atctgcgagc atttaaaacg tgacggttta gaagattata
    10801 tccgcagcaa taccggtctg gtgattgacc cgtacttttc tggcaccaaa gtgaagtgga
    10861 tcctcgacca tgtggaaggc tctcgcgagc gtgcgcgtcg tggtgaattg ctgtttggta
    10921 cggttgatac gtggcttatc tggaaaatga ctcagggccg tgtccatgtg accgattaca
    10981 ccaacgcctc tcgtaccatg ttgttcaaca tccataccct ggactgggac gacaaaatgc
    11041 tggaagtgct ggatattccg cgcgagatgc tgccagaagt gcgtcgttct tccgaagtgt
    11101 acggtcagac taacattggc ggcaaaggcg gcacgcgtat tccaatctcc gggatcgccg
    11161 gtgaccagca ggccgcgctg tttggtcagt tgtgcgtgaa agaagggatg gcgaagaaca
    11221 cctatggcac tggctgcttt atgctgatga acaccggcga gaaagcggtg aaatcagaaa
    11281 acggcctgct gaccaccatc gcctgcggcc cgactggcga agtgaactat gcgctggaag
    11341 gtgcggtgtt tatggcaggc gcatccattc agtggctgcg cgatgagatg aagttgatta
    11401 acgacgccta cgattccgaa tatttcgcca ccaaagtgca aaacaccaat ggtgtgtatg
    11461 tggttccggc ctttaccggg ctgggtgcgc cgtactggga cccgtatgcg cgcggggcga
    11521 ttttcggtct gactcgtggg gtgaacgcta accacattat acgcgcgacg ctggagtcta
    11581 ttgcttatca gacgcgtgac gtgctggaag cgatgcaggc cgactctggt atccgtctgc
    11641 acgccctgcg cgtggatggt ggcgcagtag caaacaattt cctgatgcag ttccagtccg
    11701 atattctcgg cacccgcgtt gagcgcccgg aagttcgcga agtgactgca ttgggcgcgg
    11761 cctatcttgc tggtctggcg gtcggtttct ggcagaacct cgacgagctg caagagaaag
    11821 cggtgattga gcgcgagttc cgtccaggca tcgaaaccac tgagcgtaat taccgttacg
    11881 caggctggaa aaaagcggtt aaacgcgcga tggcgtggga agaacacgac gtaatgtaaa
    11941 tgccggatga agcgtttatg ccgcatccgg tagtcccgaa acgtgcgggg gcaaccccgc
    12001 acacatcaat aatccctccc ttcccctgtg ctacacttcg cgccattcct tactgcttag
    12061 agtttgctat gagacgagaa cttgccatcg aattttcccg cgtcaccgaa tctgcggcgc
    12121 tggctggcta caaatggtta ggacgcggcg ataaaaacac cgcggacggc gcggcggtaa
    12181 acgccatgcg tattatgctc aaccaggtca atattgacgg caccatcgtc attggtgaag
    12241 gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa agtcggtact ggtcgcggcg
    12301 acgcggtaga tattgctgtt gatccgattg aaggcacgcg catgacggcg atgggccagg
    12361 ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg cttcctcaat gcgccggata
    12421 tgtatatgga gaagctgatc gtcggaccgg gagccaaagg caccattgat ttgaacctgc
    12481 cgctggcgga taacctgcgc aatgttgcgg cggcgctcgg caaaccgttg agcgaactga
    12541 cggtaacaat tctggctaaa ccacgccacg atgccgttat cgctgaaatg caacaactcg
    12601 gcgtacgcgt atttgctatt ccggatggcg acgttgcggc ctcaattctc acctgtatgc
    12661 cagacagcga agttgacgtg ctgtacggta ttggtggcgc gccggaaggc gtagtttctg
    12721 cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg tctgctggcg cgtcatgacg
    12781 tcaaaggcga caacgaagag aatcgtcgca ttggcgagca ggagctggca cgctgcaaag
    12841 cgatgggcat cgaagccggt aaagtattgc gcctgggcga tatggcgcgc agcgataacg
    12901 tcatcttctc tgccaccggt attaccaaag gcgatctgct ggaaggcatt agccgcaaag
    12961 gcaatatcgc gactaccgaa acgctgctga tccgcggcaa gtcacgcacc attcgccgca
    13021 ttcagtctat ccactatctg gatcgcaagg acccggaaat gcaggtgcac atcctctgat
    13081 tgatttgatc gattgagcct tccagtcctt cgggactgga atttttttgt tcggagaacg
    13141 aagataaggc aagtcaatca aaacaggaga aaaacatggc tgattgggta acaggcaaag
    13201 tcactaaagt gcagaactgg accgacgccc tgtttagtct caccgttcac gcccccgtgc
    13261 ttccgtttac cgccgggcaa ttcaccaagc ttggccttga aatcgacggc gaacgcgtcc
    13321 agcgcgccta ctcctatgtt aactcgcccg ataatcccga tctggagttt tacctggtca
    13381 ccgtccccga tggcaaatta agcccacgac tggcggcact gaaaccaggc gatgaagtgc
    13441 aggtggttag cgaagcagct ggcttctttg ttctggagga agtaccagat tgcgaaacgc
    13501 tatggatgct ggcaaccggt acagcgattg gcccttattt atcgattctg caactaggca
    13561 aagatttaga tcgcttcaaa aatctggtcc tggttcacgc cgcacgttat gccgccgact
    13621 taagctattt gccactgatg caagaactgg aaaaacgcta cgaaggcaaa ctgcgcattc
    13681 agacggtggt cagtcgggaa acggcagcgg ggtcgctcac cggacggata ccggcattaa
    13741 ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat gaataaagaa accagccatg
    13801 tgatgctgtg cggcaatcca cagatggtgc gcgatacaca acagttgctg aaagagaccc
    13861 ggcagatgac gaaacattta cgtcgccgac cgggccatat gacagcggag cattactggt
    13921 aagcggttac ttatcgataa acggcacgat gagcaaatcc gcactcatct gattgatcat
    13981 cccgcgatat gccggcatca aacggttgat aaatgagtgg tgatgaccac agacaaggag
    14041 gtcgcactgc tctttttgca taatttccag cagtgtttcc ggcatttctc cgcgttcaat
    14101 acgcagtttt gtcttcggcc attgaatatt tttcgtcagt ttatacagct tgttatccga
    14161 cttattcttc aacaattgaa gaatatcttc tgttgcaggg aagtagatac ccggatacaa
    14221 ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga gcgtcattat gtctggcgag
    14281 ctccagggct ttattcacca gtaaggcatc ttcttcattc ccggaaattg ccacgccaat
    14341 gtgtttataa gccatgttta actccttcta aagcaactcc atcaagctaa cgaacgcagt
    14401 gataactcaa aaataatcat ctcggcctgg catgagaaag tgaaggccgc atcaagctca
    14461 acttgcccct ccagatcctt aatcttatag gccacgctgg ccccttcatc tgcagaggat
    14521 gcagcggcgg caagctcttt cagtttggtc ataaaagact ctgcttcttc gcgggtggca
    14581 aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa tcaccgtacc gatatcaatc
    14641 gcacatcctt tagtactgca tttatctacg acatctttca tagggcacct ctgtatattc
    14701 gcctctctgt cgattacgac ttaagaataa tgcgccgtta agggaagaac aaacaagtaa
    14761 tgatcttatg gattaccatt ttttttgata ttgcttaagg tgaagagttc agaaggattt
    14821 tccgggtatt gtcatttgca tcgtggaaat aacaatcccg gaaaaaaata gcagtaagag
    14881 atatattaaa ggttaattac ttgatttatt gtcggcttta tacttcacat cctgagtatc
    14941 tttaccatat ttattttcgc cttgtgtgcc aacaaacgca ccaagatcga taagcatcat
    15001 caccagaatc aacgtcggga caaaacgccc caccgcccat tgccagacac ccggtaaaat
    15061 cgcccagtta cccgccagca gcatccacgc cacaatcatc agaaatgccc atgcgccgga
    15121 acgcccgcga tcatgcaagc gcttaacagt tactgccgct gttggccaaa gcaagcacac
    15181 aaggcaaaac gccgcggtct gaatatcgag taaattttta cccgccagtg agaaaagcac
    15241 cagcatgcct gcgaaccaca ggcctatcca aatccagaaa tcacggcgtc caatacgccc
    15301 tttaaatgag aataaccatt gctgtatggt catgtaagtt ccttgatggt tgtcttttcc
    15361 aggattctac ccttttgaca agggcgacag atatcgtttt aatcggagcc agtcataaca
    15421 aaaggtactg tcaatgaagc cagggtgtac gctgtttttt ctcttatgtt ctgcattaac
    15481 cgttacaaca acggcgcatg cacaaacacc agatacggca acgaccgcgc cttatctgct
    15541 ggctggagcc cctactttcg atctctccat cagccagttt cgagaagact ttaacagcca
    15601 gaatcctagc ctgccactga acgaatttcg tgccatcgac agcagtcccg acaaagccaa
    15661 tctcactcgt gctgcaagta aaattaacga gaacttgtat gcttctacag cgctggagcg
    15721 cggtacctta aaaatcaaaa gcattcaaat gacctggcta cccatccagg ggccagagca
    15781 aaaagcagcg aaagcgaaag ctcaggaata catggcagca gtgatccgca cactcacccc
    15841 attaatgacc aaaacacaaa gccagaaaaa actgcagtcg ctactaacgg cggggaaaaa
    15901 caaacgttat tacaccgaga cagaaggtgc actgcgttat gttgtcgcgg acaacggcga
    15961 aaaggggctg accttcgctg ttgaaccgat taagctggcg ctatctgaat cgcttgaagg
    16021 tttgaataaa tgacaaaaag caaagccttt gtgccgatga atctctatac tgtttcacag
    16081 acctgctgcc ctgcggggcg gccatcttcc tttattcgct tataagcgtg gagaattaaa
    16141 atgcgacatc ctttagtgat gggtaactgg aaactgaacg gcagccgcca catggttcac
    16201 gagctggttt ctaacctgcg taaagagctg gcaggtgttg ctggctgtgc ggttgcaatc
    16261 gcaccaccgg aaatgtacat cgatatggcg aagcgcgaag ctgaaggcag ccacatcatg
    16321 ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat tcaccggtga aacttctgcc
    16381 gctatgctga aagacatcgg cgctcagtac atcatcatcg gccactctga acgtcgtact
    16441 taccacaaag agtctgacga actgatcgcg aaaaaattcg cggtgctgaa agagcagggc
    16501 ctgactccgg ttctgtgcat cggtgaaacc gaagctgaaa acgaagcggg caaaactgaa
    16561 gaagtttgcg cacgtcagat cgacgcggta ctgaaaactc agggtgctgc ggcattcgaa
    16621 ggtgcggtta tcgcttacga acctgtatgg gcaatcggta ctggcaaatc tgcaactccg
    16681 gctcaggcac aggctgttca caaattcatc cgtgaccaca tcgctaaagt tgacgctaac
    16741 atcgctgaac aagtgatcat tcagtacggc ggctctgtaa acgcgtctaa cgctgcagaa
    16801 ctgtttgctc agccagatat cgacggcgcg ctggttggcg gtgcttctct gaaagctgac
    16861 gctttcgcag tgatcgttaa agctgcagaa gcggctaaac aggcttaagt ctgacaggtg
    16921 ccggatttca tatccggcac ttactttcct taactcttcg ccttaacgca aaatctcaca
    16981 ctgatgatcc tgaatttcct cggctgaagc acggttaagc gtcagtagat ttcgttgtgt
    17041 cgccagcaat acaaatgagt tatcactctg ccgtaccatc gccagcccgt agcttcccat
    17101 atgttcccgc gcctcaggta cttcttctgc cagcatcata aatgggctgc gttgtaccag
    17161 ttcgctttcc gttacccgac gcgccaggta ttcatgcccg cgcaaaccac ctggcagtgg
    17221 caaccagcgg ctgctgatgt tcgccagatt gttatccagt tgttcgcgca catcaggacg
    17281 aatacaagag atatgaatat gaaaatggtt ttgcgtacgc ccggtgcggg agttgatcgc
    17341 caaagaaacc gcgcgatcgg gaaccggctg gccgtatttt ttgctcataa aatcacgcgc
    17401 ctgccaggcc aaccaaaaga agttcggcgt tgaaggatcg gtcaacaaag gactttcagt
    17461 accgttaata cgatacgttg gcatcaacag atattgcagt ggaccattaa gatcttttaa
    17521 aaccacgtat ccggcattgg gtttgacttc cgcacatggc gaaggatttt gattttgctg
    17581 ctgattgggc aaacattcct caaggacaat cttacgtaat gtatccgact cttcaccggt
    17641 taatttccag taaccaatac cggcagccac aacggcgata actatcatca ccaaaaaaag
    17701 aagacccgct tttttcatct ttttttccct gtacctcaaa gagatgcaag ggtaacgcaa
    17761 aatcgtgaca aataaaaaac ccggtcgcga gaaaccaccg ggtttgataa ttatcctggg
    17821 gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt agcaaaatgc tctttttgcg
    17881 ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa cttcagcttt ggaaacgcat
    17941 tttcgtactt tttcgctact tcagcgtcgc gcggacggta gtagtttttc gcggcaattt
    18001 cctgaccttc tggcgagtag agatatttca ggtaggcttc cgccacctct ttggtgcctt
    18061 ttttctcgac cactttatcg accaccgaca cggttggctc tgcgaggata gactcactcg
    18121 gcgtgacgat ttcgaattta tctttcccca gttcattcgc tgccagcaga gcttcgtttt
    18181 cccaggcgat cagtacatcg ccaatcccgc gctcgacaaa ggtgttagtg gagccgcgcg
    18241 cgccagaatc cagaacttcg acgtttttat acagtgcccg aacaaaatcc tgtgcttttg
    18301 cctgatcgtt gttgttgtga tgcagcgcgt agccccaggc agccaggtag ttccagcgtg
    18361 cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac acccggttta atcagatcgt
    18421 tccagtcatg gatctgcttc ggattgccct tacgcaccag gaaaacaatg gtggaagtgt
    18481 acggtgcgga gttatccggc agacgtttga tccactcttt atcaatccgc ccgcgttccg
    18541 caatcgcgtc cacgtcatag gccagagcca gcgtgacaac atcagcttca ataccgttga
    18601 ttaccgacgt cgcttgttta cctgaaccac cgtgtgactg acgaatcacc acgttatcac
    18661 cagtttgctg tttccagtgg gcgctgaatg ccttgttgta ctgttcgtac aattcgcgcg
    18721 ttggatcata tgaaacgtta agaagctgaa tatcctttgc cataacgctg gttgccgcca
    18781 gcaaaaatgt taaccctacg ccccacttgt tcatcgcccg actctcttat gttgtgttgt
    18841 gatgagcaaa gcgtgccaga aggttaacca aacattaaag aataaaaaaa gattggctat
    18901 aacttgcggg tatatgttga gggattaaaa aggcggaaga aatccgcctc atattgctga
    18961 caaagtgcgc tttgtccatg ccggatgcgg cgcgaacgcc ttatccggcc tacaaaagtt
    19021 tgcaaattca ataaattgca gaattcatgt aggcctgata agcgaagcgc atcaggcatt
    19081 tttgcttctg tcatcggttt caggctaaag gaatctgcct ttttccgaaa tcattaatac
    19141 agttttttcg cgcagtccag ccagtcacct ttgaacggac gcttcatgtt ttcgatagcg
    19201 tcgatgatgt cgtggtgaac cagctgttcg ttctggatac ctacacaacg accgccgtaa
    19261 cctgccagca gcagatcgat agcgtaagcg cccatacggg aagccagaat acggtcgtaa
    19321 ggcaccggag aaccaccgcg ctggatgtgg cccagcacag ttgcgcgggt ttcacgaccg
    19381 gtttctttct cgatgaaatg cgccagttcg tcaacatcac acatatgttc ggtaatcgcc
    19441 acgatcgcgt gttttttacc tttcgcgata cccgctttga tttcgtttac caggtcttca
    19501 cggctgaatt caacttccgg aaccacaacg aattcacagc caccggcaat ggccgcagcc
    19561 aacgtcagat cgccacaata acggcccatc acttccacca cggaaatacg ctggtgagaa
    19621 gaagaggtgt cacgcagacg gtcgatcgct tctacaacgg tgctcagcgc agtgaagaaa
    19681 ccgatagtgt agtcagtgcc tttgatgtcg ttgtcgatag tgcccggcag accgatgcac
    19741 gggaagccca tttcggtcag acgcattgca cccatgtagg aaccgtcacc gccgataacc
    19801 accagcgcgt cgataccacg ttttttcagg ttttcgatag ccacggcgcg gatgttctcg
    19861 tcgcggaatt ccgggaagcg cgcagaaccg aggaacgtac cgccacggtt gatcatgtca
    19921 gaaacgctgt aacggtctag ctgtaccata cggtcttcat acagacccag atagccgtca
    19981 taaatgccca ttacttccag accttctgtc agcgcagaac gaacaacccc gcgaattgcg
    20041 gcgttcatgc ctggcgcatc accgccgctt gtcaacacac cgattttctt aatcatgact
    20101 acctctgaac tttggaatgc aaaataaaat ctgttgccgg aagtcttctt gcacatcgaa
    20161 gtgatccaac gaatgtgcaa atagtataac aatcacttcc tgctgaattg attcaggtca
    20221 ggccaaatgg cggtatttta tacacaaaat gcgggtctgg ctctctttta tactgattat
    20281 gaaagcatag accgtttacc ctccctgggt acgacggaac aggggtcctg atggataatt
    20341 acatccgatc ccggaaaacg ccgtaaaata gcctgctcta cctgatccgc caccatatgt
    20401 gcctgaacca aaggcagaga gtcttccatt tccaaatgaa tctgaataaa gcgggtcggc
    20461 cctgactgcc gcgtgcgaag atcgtgagcg ccgctaacac ccggccagga agtcacgata
    20521 tcaataattt cttgccgttc ctcatcaggc aatgcgcgat ccagtaatga ctgtaccgcc
    20581 tcatatccca tgcgtaacgc gctatataaa atatagatgc cgattcccaa tgcaaacaga
    20641 gcatcggcgc gatgccagcc gtaccaggac aaccccagcg ccagcagaat tgcgccgttc
    20701 atcataacat cagactggta atgtagcata tcagcccgca ccgcctggct ttgcgtccgg
    20761 cgcaccaccc accgctgaaa cgagacaagg ataatcgtac aaattagcgc cacaattgtc
    20821 acgataaccc cgacgcctgg atctgtcatc ggtgttggag ataccagatg ttgaataccc
    20881 gtcaaaaaca ggaatagtgc cgaaccggag ataaacatac tttgcgccag cgccgcgagg
    20941 gactcagctt taccgtgacc aaacgagtga ttatcgtcgg caggttgcag ggaatatcgc
    21001 accaccagta aattcgtcaa cgacgcgccg atatccacca gcgaatccac cagcgcggcg
    21061 agaatactca ccgacccggt ataccaccat gcaaaaattt taatcagcaa tagcagcgaa
    21121 gccatcgccg tcgcagcaat cgccgcccga ctgaccagcc gtccataaga ttgattcata
    21181 aatactcccg ctatcaactg acgctagtat aacggaagca aatcatctgc aatgcattaa
    21241 gcagcaggca aattgaggat aaaaaaaccc ccacatcatg tgggggaaga cagggatggt
    21301 gtcacaaaaa agtacctaac ctgatgatat aaatggattt atgtcaaccg ctgtccacat
    21361 agcgtccaca tcgaccataa ataagcccct caactgaggg gctatttttt tgatcacatc
    21421 cacataattt gctgccctga cggcaacggg tgcggcctta cggcgtggac ttctcccggc
    21481 ttcacgatgt atcgctgtac cgactcataa gtgatgaacg tggcgctgca attcacgttc
    21541 tggcactggt gataacgctc ttttgtcgtg tcagtgatat agcgacttgt acgcgcatgt
    21601 gcggcatgct ggcataaagg acaatgaaac atcgcgagca cctcttccgg ttttgttgat
    21661 ggtgccattt tagttaattt atccttataa aacaaacaga taaataaacc acgtcactca
    21721 tcatcttctg tttcgtactc cacatcagaa agccttacct caagctccaa ggacgtcgtg
    21781 aagccgctat tattgagaca atgtgtcacc ttagtgatgg tccagtcctg ctcgtctatg
    21841 acgcgcttaa agcctgacac tttaaccggt gtttccgtgt aaatatctgc acgaccggta
    21901 gccaggctga tggagaactc cgctacaccc cgttgcagtt tatcccactt cgcctgagcg
    21961 gcgcgcatgg cctgcgcttt tgtggcatat accgtggtca gggcaaaaac gttgtcagct
    22021 tcaccggcca tgtattcacc ttcgcgcgct tccggtactt ttggcgcttt cttctgcgtg
    22081 accggtttcg ctttcgggtg ctccagtgcg cgcaggtgtt tttctttctt tttgcgtttc
    22141 agttttacct tctgcttttg cggcttcggg tctttggtgt gtaaccactt tgccgttacg
    22201 ccggtgtaag ctccacggtc agcaatcgca aaatgatgac ggtcgccgtc gctgcgggtg
    22261 atggtaatct gcgggatttt tttaccgctg gccgtcaccc cctgccccgc tttgagaaac
    22321 agcagttttc ccatttttac cgacacctcg ccaccgttgc gttcagcaag acgggtcagg
    22381 aatttcgcat cagactcctg cgactggtcg atgtgtggaa tttttattcc ggccagtgac
    22441 ggagcgacac tggcttccag cctgttacgg gaggctatcg cctcaacaat cgcaccgagc
    22501 gtaatgtcat gccatgagcc ttcacggcgg gaattgagcg tcccgcgaaa atctgcactc
    22561 cgggcgcgga tggtgaccac atccggcgcg ccccggtgtt caacctcatc aacggtaaat
    22621 ttccctttgc ataccagggc aaaacctttc cagccgatat acaccgtcag gacagcgcca
    22681 cgaaccggca gcccgacctg cccgtcggca tcgttcagtt caatatcaag ctggtcagcc
    22741 tcaaagcccc ggttatccgt cagggtcatg ctcatcagac ggtcgctgat attgccggta
    22801 atatccctgc tgtcgagcat cagcatgtaa tccggcgtca gcgtactgcc tgcatcaaat
    22861 gtcagcgcat ccagcattat cccgcccccg tcatacccgt gaatttagtc gccatactgc
    22921 cagccttacc gatgagcgat tccgcctgtt taccgatatc gccataaagc gcggccagtg
    22981 actcatcaac gcgggtgagc gacagcgtaa aatcaatttt ccgtggtgtg ccgtctgcaa
    23041 agaaaatact ccctgtttca ctcaccttgc tgatgacata catgccgtaa atcatgccgg
    23101 tgccatccag caacggccac gcccggcctt cctctgccat cagtctgagc gtggtcatcg
    23161 tcagctttcc gccagtcagt tcgggataaa gcacaccggc aagtgtgatg ttctcctcac
    23221 ctacaccgag aaactgatag gcatcccgtt taccgatacg ggaatttgac ggccagcgat
    23281 aatctgattc acgctgcatg gtctggtgtg gcagcgtctg gcgcataaaa acaaacatac
    23341 ctaacgcgag catcattttt cgtcacctcc ttaaccgtca tgcatcatgc tggcacgggc
    23401 gcgcgcacgt ttatcccgct cgtatttttc gagcgcatcc tgtaactggc ggtcaagctg
    23461 tgtccccggt gcagtaccac ccgtcaggct gatgtgatat tcgtttttac tctggtccac
    23521 ataagagcgg ccagccggtg ccgtgaccgg ctgataagcc tgatagcctg cataagagct
    23581 ggtcgccgga atataaccac cggtgccata cgtggcggca tgagttctgg cggcggtctg
    23641 gtcaagtgtg tctgactctt tgttgatgac cccgagcttt tccagtaccc agtcaatgcc
    23701 gctgcgcaat ttgttgaacg cattaagcgg cagcatcagc gcgtcagcca gtgcctgccc
    23761 gaacatgacg cccgtgtcac ggcaacggtt cagggtgtcc tgggtggctt tgaccggggc
    23821 aatcaggttt ttaaaccact gccacgcagc ctgtaacttt tcgcccagcc agtcaaacac
    23881 cggtttcagt ggcgtgaaca gctcccccac cggcgcaaat gccgctttca gcccttcaac
    23941 cacaccgcca aagaatgcgc tgacaggctc ccagtattta cggataagca acgccccggc
    24001 gacaatggcg gccaccacag ccacaaccgg ccagctaatc gccccgatgg ccgtcataac
    24061 ggcactacca accgtcgtga agattgcccc cattgcgcct gctgccgcga tgatggcatt
    24121 gatgccggtg ataaccggcc aggctacaag accaattgca ccgatgatgc cagtcagcgc
    24181 cagcgcgcca ccggcaatga ggccaatggt tgacgccagt gatttgtttt tctgtaccca
    24241 gccgtcgagt tttaacacat actttgtggc cgtctgcgtg agcttacgta gcgcgccttc
    24301 ctgctggtca aacaggtctg tccccaccgc ctcataagcg gactgaaact ccttaaagtc
    24361 accgccgagg ttgtcctgca tgatatttac cagctctgcg gtcttcccgt ctgaggcttt
    24421 aaacgcagcg gtcagtttgt ccagctttcc ggttgacgcg gcagtcatca gcacagcggc
    24481 ggctgagctg gcctcctccc cgaaaatggt tttcatgtat tcggcctgct gggcagtacc
    24541 gagccggttt ttctcaaagc tggcctgcat ttctttcaga atggtaaata ctggtcgggt
    24601 gtttccctta ctgtctgagg ttttcacgcc aagctctttg agtgcatccc atgcttttcc
    24661 agtcggtgcc tgcaggcgac ttaacacggc acggcttccc gtccccgcca ttgagcctgt
    24721 gatttttgca tcatgcagcg ccccgaccat tgcggcggtt tcttcaatgc tgacaccggc
    24781 attttttgcc actggtgcgg cataggtcag cgcatcgctc atgccgtcaa aatcagcggc
    24841 ggttttgttc atcgtcatgg agagaacatc cccgatatga gcgaccttat cgtttgaaag
    24901 ctgaaaggcg gatttcatcc ccatcagcag ggcggcgttt tcttccattg tgcggcggtt
    24961 cgccagcgcc atattcagcg tgaccggcgt tgccgcctga atggcatcaa catccccacc
    25021 cgctttcgca atgataatct gtgcaccggc cgcatcatcc gctgaggcgg cggtgttgtc
    25081 accgagctgg cgcgcctgct tgcggagtgc agccatttcg gcggagtctt ttgccacacc
    25141 gagcacagcc tgcaattctg agtttttctg cgcaaactca taacctggca tcagcagctt
    25201 aactccggct atcgttcccg cagcagcaat ccccacaccg gcagcgccca ctgaggccat
    25261 atttccggcc agttcctttc cggcctgata acgctgtttg actgcgttaa gttttgcctg
    25321 ttgcgcactg acacgcgcca gcgcgtcacg ctgccggtta agctgggcgg tggtttcact
    25381 gatacggttt ttcagtccct gctcatcatg tgcaagattg cgggtattaa ttcccacagc
    25441 ggccagttcc cgctgctggc gtttaacgga atctgtcagg cggttatatt tcgcctgtaa
    25501 gtcctccgcc gcacgctttg cggattccag cactttcgcc tgagcacggg tcgggcgttc
    25561 ggtgttttta aactgtgtgg caagggcttc ggcctcctgc cgtgcctttt caagtgcatg
    25621 accagtcacg gcgagctgtg cactggtctt gcggaatccc tcaatacggg atgcgtgacc
    25681 gttcagctcg cgcagtgatt tttgtgtttc ccggatatcc cccgacagca acttactcgc
    25741 tctgcggatg gatttaaacg ggcgggacgc ctggtcaaca gccctgagca atacctgtaa
    25801 ttttacattg ttactcattc gtgtttccgc ttcgcctgag cgccttttcg cgccatgtga
    25861 tgagttcggt caggctcatg ggatacagtt ctgatggcgg ccagtgaaat atcactgcca
    25921 catccgccat caggtcatcg accgacagat ttttcggaag cgtcactgca ccgagttcgg
    25981 cgacaaaaaa ccgaccacct taccggccag cgccacaagg tcaggcagtt ccagcgcggc
    26041 gacttcctgc tcggtcagca tcggtgccgt catgcgcggc agcaccttaa tcagtgcatc
    26101 gacttctgag tttgcgaccg cagccagact gacaccgcgc agcgtccccg cactgggttt
    26161 catcagcgtg acctgttcga taacctgctc accacgcttg accggattgt ccagggtaat
    26221 cacattttct ttgttcatgg ttttctcact tctgaatcgg ggttaaccgg tcagccaggc
    26281 tgaccggatg aaaattacag gccgatattg cggcggtgtt gctccagccg gtcgacgccg
    26341 ttcaccttct caatcatgtt gatggtgtcg atttcgacca gctccttacc gtccatcgtc
    26401 agccggaaat aggtgcagac cacggagatt ttcgactcgg tgtcttctcc ctgtttaccc
    26461 tcgccggtgt cgatttcttt ctgacggcca cgcatgacca cctctacggc caccgtttcg
    26521 ccggtatcgt cacgctggta agagcctgca aaacgaatcg gcacggcatc cacaccggtt
    26581 gcggcgtaaa gctcccagat aaccgaatcc gggaagcccc cgagcgacca ctccattgac
    26641 agcgcatcgt catcaaggcc gaggtctacc ggtgcgcttc cgttcatccc cgcaccgcga
    26701 tagttttcga gtttacgggt cagttttggc agcgtgacgg actttgcaac gccctgatag
    26761 ctgtagccgt tcagaaagac gttcattaac ttgagtttgc gcggcattgc catcggtcag
    26821 gctccttaat tgctgttaac cgaggtgacc agatttgcca ggtatttatc ggtaatacgc
    26881 tggcgcaggg tcaggttttc aagaggaggc accggggtat agtcgtagtc gatatacagt
    26941 tttccggcct tgagggtttc cgcatcgttg gattcttcgc tgaaccagca ggtcgcatcc
    27001 acgatatagc cgtttgtttt cagctcacgg aatttggcat tgatgccgtc aacgatgtcg
    27061 cgaatcagcg ttgcggtgat gggcttgtcc accgcccaca tgtgcgcctc agccatcgtg
    27121 tcggccagca cctgcgcggt gcgggtgtag ttttcaaaga ggaacagcgg gtcatcagag
    27181 caggtacggt taccccagaa gcggaaaccg tcacggcgaa tcagcgttgt gacgcctgac
    27241 tcgttaagca ggtcagcatc ggtgccggac tcctgcaaat cccagaatac ggacgcgctg
    27301 atgccggtaa caccgttcac cccgacgttg gacagcgttt tatgccagcc ctgctcctgg
    27361 tcgattttag cgcgcagacc cagcgcacgg gcggtggcat acgcggtggc ggtggtactg
    27421 gcgaccgtat cccatgcgag gaaatccggc cagatgacca tcagctcacg ctggctgaaa
    27481 ttctgacggt aggctttcac ctcggaaacg gtcttacagc cccatgcgct gatatacccg
    27541 aaagcgcgca gcttctgaca gactgatgcc agtgcaacag ccacctcttt ggtgtccagt
    27601 cccggcacgc cgagaatacg cggtttaaca ccggttaccg actccgccgc cagcagggct
    27661 ttcagtccgg tgtactgacc gttttcgtcg gtggtgccga tgatattgga aacggtctgc
    27721 gcgagtttcg tttcctcgtc gtcgccggtg ccgtcttcca cacgcacgac aacggtgacc
    27781 ggttttgact ggtcggcgat ggcctgcaac gatgccgcca gcgtgccttt tttaccggcc
    27841 tttgcaattg cgctctgcac attggtaatc agcaccggtt tattgagggg gaaggtttcc
    27901 gcatccgcat cgctggccgt gcagaccatg ccgacaatgg cagtggatac ggtggaaatg
    27961 acgcgggtgc cgtcgttaat ctccagcacc tgcacgccgt gatgatagtc actcatccgt
    28021 ttaactccgt ggttaatggg tgcaactatt ttctgttggg cagtgcatga gacgctattt
    28081 gacctggctg gtcagtggat gaaacaacag ataaagaaaa tgcgggcaat tcgcccgcat
    28141 gtcctgattt gtacacactc attttccgac tgacagttta catagcccaa acgctatcaa
    28201 atctgacagc ctgctatgag cgaaaagcgt taaatctcac gatatctgct accgggaaaa
    28261 atttcgatta tcttcgatgg gaactttgat aatgtagtaa atgccatttt cagcgatcac
    28321 ttttcaagcg tgtgtaaatg attaatgtta aaaaattgac ttcactgaaa taaggttgct
    28381 agtatttttg gagcaaccat taatgttttt tatttaagga atgtaaatga catacgcact
    28441 gattgataat tcaactctaa ctgcagtcca aagaatcgag gggaaagtaa aaacaagatc
    28501 gaaggactca gtagatacag acataatagc atttgagaac tatatacagt ctattttgtt
    28561 ctatgatcac gttatcgcgg ttgatgatta tattccagag taccgtggga gtaggatttc
    28621 aagtttcaga gagatattat ttcttgataa agcacaatat gggttggatg aaattgaaag
    28681 tgaagcaaaa aaaatatctg atgcattgaa acctaaaatt cgtggtggaa catttgttaa
    28741 tgacgatttt aaaaaactaa ttgaacttct tcagacacat atcatatgta catgggacat
    28801 tagttcaagt gtttatcatc taacactgaa gaacttatct gatggaggta aggagtttga
    28861 aaaatatggt aacattgctg ctgccatttt ttcagaactg cgtgatgctg ctgagtctgg
    28921 taatagaaca aatggcgatg ttgaacttct cgatcgattt ggcaacccta ttcgcaaggg
    28981 gtacaaagtt cccggagcaa aatgggggga tggcacatct gctggtgaag catcagatgc
    29041 aatcaaagca ttcgtggctt cgcttgtatg gctggctaat cgggcagtat tttattctct
    29101 cacagctaag taccttcaag cagatacttt tttatatcca ttacgtcagg cgtatcaaca
    29161 gacatattta agccaaaact gtcagtatgg ttttgactat gctaaacata ttgtagaaaa
    29221 cttttccact tcgttaagtc atgatctcat tgatattcat tctggtggta aagctgtagc
    29281 aacagcaact tcgcttcctt tattttcggc atggctcgca aaagaaactg gtgatccaag
    29341 ttgtattgtt gaagctgctt ataacataag ggacaacaac gaatttgtaa gggcccgtga
    29401 gaaaatgcga gaaataaagc gcttgtttga tgaaaatgaa ttgcctcaag ctaataagtc
    29461 agtaagagca ttgcttactg aagtatcgaa agaatcagat tatattcgaa ctaagtatgg
    29521 gctcaagact agacaaggtg tacctgtaac taagcttgta catgtataca atactgttgc
    29581 ggctattaat tcccttccga aactaccgac ctttaatttt aaagttaaag ttcctgattt
    29641 cttatatgat cttaaaaaac ccaaggggtt tcgtgctctc tatcgaaatt tgactaatga
    29701 tctttctact gtgtggtcgt taggagaagc tcgagatatt cttggaagta aagttgtgat
    29761 agataatcat gcaagaattt acagtccgaa gcatgaagaa ccgcgtttca aaaactctca
    29821 ttccccatat aagagcccga tgtagcttaa gaaaaagaca cgcccacctt gtgcgtctac
    29881 ttctggcaca gagcggactc tcagattagg ctttactctg tgccatagat atgtaagctc
    29941 acaccagagc tcatacaact tattgcggca tttccggcca ttcaggattt gcaggatcca
    30001 cacgactgac cagaacactg tagcgttccc atgcctccag tcgactgcgc tcctcatctg
    30061 ttgccatatt cagcctgaca gcgcgctcca gcggcaaaat cacggattca gcatctgcaa
    30121 gaagtctggc tttccgattt tctgcctgct gctgcaattc ctctgccgta taaattcgtt
    30181 taatcactgt acagtcctta aacatccagt tccctgaaat gtccgcccgt cggttagcag
    30241 taatatccgg cacttcaaca acacttaatc catccggtct gatagctgtc acatcctttt
    30301 ccacatagcg gataatatta tctttgtcgt acgctatttt tatcgtgtca tcagcaaaat
    30361 acttttgttc ttcgtaccag ttcttaccat cttctgaaaa aaaccagaca acatcaaagt
    30421 cctttgtcaa ttgatattgt tcaaccgttt ttggattacc tgccgttata tttatcaaat
    30481 gctgcataaa ttatacctgt gccacgttat accaagtccc gttaatgtat ttctgcaccg
    30541 gtctgtaata tacgccacca atgttatcgg cagagtttga gccggtatcc tgaacaataa
    30601 tgccggaata tacacacccg gacggtgcct gatgtgtcca tgtcatacca ttgttcgcag
    30661 gtttgtatgt ggcagcacca ccaagccgga tatcccggac atagcgtgaa tcaaagttgc
    30721 catagttaga tggtgatacc tgcccgttaa cagcaaaagt gatgctgtta tctgtatttc
    30781 tctgactgta aaaatgccag cctgcatcat cacctaattc agccaccaca ggacgacttg
    30841 agtttcccca caaattgaat gcggcttcct tcgtggatgt attgctgctg ctgaccgtga
    30901 actttttccc gctaccggca cgtactttgg tacttgagac aatatcacct gtaacactca
    30961 ggccatgccc cattgacact ccgccattgg cgttattgat agtcagcggt cttaagccgt
    31021 tccatgtccc aaatttatcg ccagaggccg tcagcattaa atatgtgctg ccgccatcat
    31081 tcctgataaa gaatccataa ttgccataag caatgcgcag accattagca ctgagtgatg
    31141 taatttcacc tcttgaacgg agaccataag cggagctgag tgataattct tcctgagcgt
    31201 cattgtttcc tgtcgcccag cgaattaccc cgccctgaac tgtttcatgc cagatagtat
    31261 ctccttctcc accacgaaac tttctgagat attttttgcc gcctctggtg cctgaacata
    31321 aggccgtaga catataggca ttctggcttc cgccatcctg attaatcgtt ccggtcattg
    31381 agtcgccctg acgattccag tcacgacgcc agccggggga gtagccgtcc ccatgattta
    31441 tgtaagtgaa ttgcgcgctg gttgtaccgc caccgcttga tgttgttggc gtggtcactc
    31501 ggatagtgat tgcagattta gttcccatga cctcgacgac acaaccagcc aggtggatat
    31561 caccacatcc ggtatccgta atgattttgt tatttgcata tgaccaggag cctttgcaca
    31621 tccagtacgg atgattaaat gcaccacggg aatccagcca ttcaataaac tgagcggttg
    31681 tccagtttcc ggcttcagtg ctcaaagcgc cgctataagc acgacaggca ccgatatttt
    31741 tcgtgaaggt atcctttccc ggaatatccg caccgttctg atctttctga agacgttttt
    31801 cagcattgtc ataggcagac ttcactgctt ttggcgttgc ggccagcgtt tcagaatcgc
    31861 tgttgatggc actacttagc tggacaagac cttttcgcgc tgtggtggca tcctgtgcag
    31921 tgtatttccc gttagcaagg tcatacgctg tcttaaccgc ctttggtgtt gccgcaagcg
    31981 tttcagaatc gctgttggtg gcgctactga gctggacaag accttttcgc gctgtagtgg
    32041 cgtcctgtgc agtgtatttc ccgttagcaa ggtcatacgc tgtcttaacc gcctttggcg
    32101 ttgccgcaag cgtttcagac gtgctgttgg tggcactact gagctggaca agaccttttc
    32161 gagctgtggt agcgtcctgt gcggtatatt tcccgttagc aaggtcatag gcggccttta
    32221 ccgctttcgg cgttgcggcc agtgtttcag acgtgctgtt ggtcgcactg cttaactgag
    32281 taaaaccttt tgcggtcagc gaggcgtccg ggtgacgtcg tgactgttcg tgctctgcaa
    32341 ttttgtcatc aacgtaatcc tgtgtcgcca tcaccgttgt ggtgtcaatg gtcagctcca
    32401 ccgaggccac actgctgacg atgatgacca tgcgacaggt ctgcgaacgc cctgagcctt
    32461 cggcaagagc tggcttataa ctttcggcca tgttcgccac ggcaattaac gttcccgcat
    32521 catcgtacag gccaagctca cgcatccaga aaccgcccac ctccggcgga ataaccagct
    32581 ctgcgataat ataattactg tttcgtttgt cctggctgat tttgttcagc gtatgtcgcc
    32641 agacttcgtg gataagcccg gtctgtccgg catccgggac aggcaattta ccaccgccat
    32701 ccccgacggc catcgtggta atgttgacct tccgccctcc cggtgcggtt gccgctgcca
    32761 gctttgctgc accggcagtg gtgataacgg ttctgaattt tgtgctcatt attcctcact
    32821 tatccggggt aaaccgtaat tacatcgccg tcgtaagcca caccaccggc gaacaggtag
    32881 ccgggaatgt cccgggtaat gttcaggcca ataagatgac ggcttgcagg tttggcatca
    32941 gcaataagcc gttccatttc ctgatacatt gcctctgtga tgccgctttc cagtacacca
    33001 atatcaagcc ggaaggtgcc gggcgggtca ctgttttccc accactccgt cacgttgatg
    33061 agatagccga gcggctccac cacacgccgg attgcgccga tagtgccttt atgacagtgg
    33121 atgaaatagg catcgcggat aacggcgcgt tttgtcgctt ccggccactt ttcatcccac
    33181 ctgtcgaccg aaaacgccca cgccagccac ggcagcagat ttgccggaca ggtatccggg
    33241 ttccacagtt cacgaatcct gaccggcgtt ttttcaattt ccgcacaggc ttttgcggcg
    33301 gcaacttcaa gcggtgatga gccggtcggc agcagtcgcg aatcactcat ccgagcctcc
    33361 ggtcacgacg cggtattcgg tacagaaaga cgcctgcgta ctgttgagca cgatgtcggc
    33421 cagcggtgca gtcagttcga cacgctgcac gccttccaca tgcaaagcgg cataaatggc
    33481 agacagacgg atgtcgcgcc ccagccggtg ctgtgccgtg atgtacgctt ccagtttttt
    33541 cacggcagca gcgcgtatgg gttcgctttc gggaccaggg taaaggtaaa gcgtggcgtt
    33601 tatctggtat tcaacgatgg cggcagactg cacggtcacg cggtcggcca ccggcctgac
    33661 gtcctcacca ttaagggcgt tacgcaccac cgccagcagg tcttcggatg cgacaccgtt
    33721 attttcacgt gacagcacag agatggtgac gcaggccgga gacggactgg ttacagaaat
    33781 atccgcgaca cgcccgtcgg cactgcgacc atgatactga taggccccca ccgacccggc
    33841 gacgcttaaa ccttcaaacg cctgctgaat acgcagacga taatccgtgt cagattccat
    33901 cactgccggt gtcggcggga gggtcgaatc atctgccggg gtgataatca ggcgcgtggt
    33961 gttgtaattg gcaccaatca catcaaggtc attaccggcg gcacaggcca gcattaccgc
    34021 ccgtgcggcc tcattcacac gctgacgcca gataagctca cgataagcat tttcctccag
    34081 cagtttgacg agaggctcgg attccagcgt cagggtacgg gcgaccgcct cctgctggtc
    34141 ttccgggtaa agggaaatca gtgtcgcctt gcgttcggca agaatggttt caaagtccag
    34201 ctcctcgacc acatccggtg cgggtagctg gttcaggtcg ataatcggca tggtttcaac
    34261 tcacagggat ggttaacgaa agtggctggc cggtgtcgtt gtgctggccg gttaacgtga
    34321 ccgtcattcg cccgtcaaag ctgcgcgccg tggtgacgga tgacagggtg acgcgggctt
    34381 cccatttcag cacggccatg taacaggcga ccttaatctg caactcaagc gccggggtct
    34441 gcggctggtc aatcattgac gccagcaacg agccgtaatc acgacgcatc acccgtgagc
    34501 cgaccggcgt gcgcaggata tcgccgatac tctggctgat atgctcaagg tcagagacag
    34561 tcaggccatc actgcgattc attccgagat aacgcgctgt catagaggac tcccggttgt
    34621 gccgccgctg tcgccggggt gtttatgggt atgcagtacc ttcccgtttg atgagagttc
    34681 accgccggtg tgttcaatgt tgccgcgcat cgtcccgccc ttctgcactt ccagcgtgcc
    34741 agtgatcagc ttgtttgtgc agaccacctc cggcgtgtcc agagtgacgc gggttgatgc
    34801 tttcaccgtg accaccggca ccgtggcagt aacagaatca gaagccgtca cgctggccgt
    34861 tttaattccg cttaccgtga gtgcactggt ttcaggttca tactcaatca ccgccccgtc
    34921 agggaaacgg atatgcaggg catccgccga cgcagacggc gcggggttat caccggaata
    34981 aatccccggc agaacgaacg ccgtgtcgag ttcaccgcct acggccagaa tcagcacctg
    35041 ttcccccacg gaaggtgccc accatgtgcg cgaacgtccg gcgcgatggg tcagccactg
    35101 aagccagtcg gtgcacatgc cgccggtctg cacacggcag cgaccggcat taaggtcggt
    35161 ttcgacgata atgccggtgc ggatcatgtt gcgcagtgcg cgcgcgagtt cctgaatatt
    35221 tgcgagagtg ttcataacgg gaaggatgcc gccgggtcat accggcggca atgtgacgat
    35281 gaggtgtcag gaatggcaca actaacggtc gaggtgagcc aggataatct cttcaatcat
    35341 ctgcacatcc tcaccggtaa agccgagcag gggacgcgcc ggataatgaa ttttcttacc
    35401 gtctttccgg gtttcttccg acagaccgaa ctgatgcaca ctggctattt tcggtgactt
    35461 cccgccgtaa aactccattg atgcctgttc agggctggcg cggatatgca aaaaacgact
    35521 ggtgataagt ttcgcaaaca tttttcgctt aacgcgaccg gtcttttttc tggcgctctg
    35581 ctgctggcgt ggtgcgtagg gtgtgccgtc cggggctttc tgtgccatca cccgacgctg
    35641 ctgactctga cgcagacgtt tcgccagttc ggcgctcagt cgccgacgcc ctgacggtga
    35701 cagcgactca ataagtccgg tcagcctgtc ttcaaaacgc ttaaactcat tcatcccact
    35761 tactcaccag ttcgccattg atataaagct ccatcgggcg ggtgaccggc tccggcggcg
    35821 gaggttccgg gatattcttc acatgcagcg cgccgtccac ctcactgacc agcgtgcgct
    35881 cggtcagcat caggctgatg ctgatatcaa agctgctgtc attgttgatg tctgcataaa
    35941 acgtgaagcc ctttttctgg ccttcgtcgg tggtcatgat gtcgggctga ttttcccgca
    36001 gccacgccag caccggcacg atgagcaggt caaaatcacc ggtaaagtcg gtcacaatca
    36061 cgttgagcgt gtaacgcttt tcgaatgaca gcgacgtcgc cagtgtggag gcaatactcc
    36121 cgttatccac gaatatccgc agcatctcgg ggcttgtttt cagcaccgtg acggcatcag
    36181 tcagcgccct gcgcaggctg tcgggtttga gcatcgtttt cgtcctgaca gtgtttaatc
    36241 atttttacct ggctggcaca gcgtgccagc gcgttctcaa gctgccggat atcagcactt
    36301 aaatcgccgt tcgtctgcgg gtcactgccc ggcatcgggc aaaggctcac tttcgggcag
    36361 gcgttgtgga caatcactgg cgtctgcgca ggctgtgcgc tggtgcaacc ggcgcacagc
    36421 atcaggcagg tcagcaccgt accagcggcg aaaatcttcg ttttcattaa gtaacctcgt
    36481 gatggttttc tcgcgctgag cttcacgctt cgcggcgttc tccagttcct gacgcagtgc
    36541 cacctgcgcc agctcgtttt tgtctgccct ggtgagggca acatgaagct ggtttttcag
    36601 catggtgatg gtcgtctgct gttcactggc gacgttgttc gccctgtcca gcaaggcgcg
    36661 caggctggca tttttgtgtt tcaccagaaa cagaccggcc accgccagcg ataacaacac
    36721 aaccagcaca atcatcagtt ttgacatggt tcccgcccct caagacgctg acggcaggcc
    36781 gtacgtatca gccggaaaaa cagcgatgcc acgagataaa tcagtgcggt aaaaatccac
    36841 ccggcagcga ccagcgagat aaacgtcgcc accatcacca tcagagccgc tgcccgtctg
    36901 cgccacggca ccggctgcaa aaacagcgac gtgacaatct tcacggccag cgattccggc
    36961 ggcagctccc gcccgtagcg ttccagcaca tactccgcgg catacacgcc gacaccaccg
    37021 gcaatcacac agataaccgt cgccagaatc gcccatgcag cgacaaaact gacggccacg
    37081 ctctgcgggt aaatcaggga cagtgccagc atcagcgcca gcgacacgtt cagcatcagt
    37141 gaaagggata atttcttcat ggtgtttact ccgtttaagc cggtacgccg ccagcggtgc
    37201 gccagacggt gaccagtttt tccagtgaat gctcacgctg accgtaaccg gcacccggca
    37261 gggacgccca gatattgcga cagcgtgaaa tggcgcgctc aatgcgtccc gcccggatgt
    37321 catccagtgc accgcgttcg cggatcaact gaatggcgag cctgtcctgt gacaacggac
    37381 tgaaatccgg cagggcaagc tgtttgcggt agtgcggcca gaacaggtaa agctgctgat
    37441 agcgaccgga ggccgtggat ttttcaccgc gacggttaaa caccttcgcc ggtcggccat
    37501 gtgcgaacgg gtggtcactg tagtcggtga agatttccgg cttcccgtcc agtccggtga
    37561 ctatcacgtc atagccccgg tttttcgtca gcggatgatt cgccgtccct tcggacacgg
    37621 ccagcatgtc gagaaaggcg gcgatattct gatgtgtgct aattaccggc attacggttt
    37681 ccccctgccc ttaaagcggc gctgaatggc aatctcaatc acctgataac cggcgatacc
    37741 cagcatggag ccgatgccgc acaccgcagg cagtgacagg tcaggaaact gcaccagaac
    37801 aacaccggca accatcgaga caaaaccacc gagcaacatg cgcccgataa acagacgcgg
    37861 agtgatgggt tcaccaccgg caagcacctt gccgacaaca atcagcaccc caatcatgaa
    37921 aagcgacagg acgctttttt cttctgctgt catgcgttac tcccacagat tgacagtttc
    37981 agccacgggc gcggtctgaa cgtcgggcag ttcgacggcg gtgccgtgtg gcagcaccgc
    38041 ccccagttca gccagtcccg gatttgcggc gagcacggcc tcgaccacgc cctcagtgcg
    38101 cccgtaatac cgaacacaaa tggcgtcgag cgtgtcgccc tgtagcgcaa aggtcttcat
    38161 cagatttgac tcacgatgca gcgcggcttg tcctggatac gcgccaccgc ccagcgcata
    38221 tcccgccaca gctcatcaat ggtgctgtca atgctgtcgg ccttcttgtc gcccttggca
    38281 ctggcatcca cgccgcggta acgctcataa agcgacgcgg tcgccatcgc acacacagcg
    38341 cgctcgtagt aaaaaacttt gatgctttca ccgtcgatgt cgtccgccgg gacgtcagcc
    38401 agacgcgaaa aaccggcagc aattttctgt tcgcggtact cgtacagctc cgcattcgtc
    38461 tccgccatgc ctgacttgat ggcctcacgc agacgggcgg gggcgacggt ctgctcaagg
    38521 cgcatacgtt cccggacgcg cttcgggtca atatcgggaa aaaagaacgt gtttttaatc
    38581 accggctcgt cgcctgccgg ttgcgggata accaccgtac cctcaccgga cacgggagcc
    38641 tcctttcgcg gaataatcag cgtcatcatg actacctctg aaaagtcggg cggtggacgc
    38701 cggtgcagtg tcaggtgatt caccgtcact gaccggcgtg ccgccctggc gcggggcgca
    38761 ttcggttgtt aactggcttt ctttttcggg cgtccacgtt ttgccggtgt cacgctccgg
    38821 gtcttacgcg gagtgcgggt ggccgctttg ggctgcggct ccggcttcgg tttcagctcc
    38881 cgctccagtc gttcaatctc ttttttgacg cctgcctgac agtcgagctg tgtcgcacgt
    38941 tgcaggtgta ccagcgcacc tgcggcatca ccagcgtcac gcagaaacag accggtgatt
    39001 ttgtgcagct ttgcgcgcac ttcatcaggc atgtcagccg tggcggtcag ttcgagggtg
    39061 tccgtcagca ggcggggatc cacagactca ccggcagcgt gagcgcgcat ggccgcaagc
    39121 gccacctcct cggtgaacat gtacggcggg gtgcggcggt gtttacccgg catggtcaga
    39181 ccgtacttca gggcataacg ggcaatctcc agcgcaccgg caatatcgcc ggtatccaga
    39241 cgccacagca tgaccgtcat cagaatgtca tcctgtgcac ctttgccctg ctccagcacg
    39301 ccgttcaccc acggcaacca gaacggcagc agttcgcgtt ttttcgcggc cttcagctct
    39361 tttgaataaa tcgctttcag tgtgcgctgg tctgcggcca gcttgaccag catctgctca
    39421 tagacagttg catgtcgcag cggggcggct tcccgctgcg cggtcatcgc tgccgagacc
    39481 cgcatcatgt ggcgctgtgc gggactcgtc atcggttacg ctcccggctc tgcggtcgct
    39541 ttagccggtg tggagaaatc accgacctta attttttcca ccagacaacc ggcggcgtag
    39601 tcctccacca cgtaatcaat gttcattgac tcgtagttct ccacgcggtc gagtttcggg
    39661 ttttccacaa tcacgcggcg atggctgtca tccatgtagt agatggacag gttttccagc
    39721 ttcgtgatga gcatcgcatc cgccgggaag tacgggacgc gtaccgccgg caggttaccg
    39781 atgcgtttct ggctgatgat gacgtcagcg gccagcatct cgctgttatc ctgctccttg
    39841 ttgacgatag ggaaatactt gtccgccagt agctgacgcc ccacaatcac cacaaggtca
    39901 gggtcttcct gataccaagg ttcaatcagg ttgttggtcg catccatcac cagtgcatcg
    39961 aggcttacat aatcaccgcc cttacccacg cggataacct cagaggtggt gcggccttcc
    40021 tcgtcagtga ccttgctcat cacgcgcgcc ggggcttcat tcggtatttc tgcagccagc
    40081 cgaccgccac atcctgcagc atcggattac tgttgcggtc agaggtttcg gcacgcttca
    40141 cgccgttaaa accggccatg atgaaatcaa gggactggcg tttgataatg gcgttacgga
    40201 tacggagctg gaaatcctga taacgcgccc acaggtcaag cgttttgtag cggatataaa
    40261 aatcgaagtt aacctggtcg cattcgtact tgtttgacgc cagcttcgag aagtccttcg
    40321 gctgacgctc ggtgccaccg gcggtgtcgg tggtgctggc gatggagccg gtgacaccga
    40381 tgccaatttt ttcccctttc atttcactga ccggcacaat gttgatgcgg gtcagaaagt
    40441 cagaggactc ctgcatggtg ttcatcaggg tctgggtgac cgacggttca acggtgaatt
    40501 ttttcgacac atcaccggcg tcgatgccgt tcagctcggc aacacgggac aggtaagcat
    40561 taaatttaaa gcgggtttcc tggcgcatag tttttcctga aattaagggt taatcgtgaa
    40621 ggttttcccg gactgactga cgccggtcag cagttcgtca tcagggcgtc accgccacca
    40681 ccggtggcct tgctgcggcg ctgctgggtc agactttcgg tgtggtcgag actgtttttc
    40741 aggcgggtga atgcctggct ggtttcatcc gccctgtcag tcacctcctg cttaagtgca
    40801 gaaaaggcgg tttccatctc agcgaggcgc tgctcagtgg cgctcagttt ttcctgcaca
    40861 tgttcagcaa cagcggtcac cgcttcatgc acgtcattca gacgggcatc atcgctggcc
    40921 tgtttgcggc caaaaatgga tttcaccttt tcggtcaggg cggtgaacac ggtttcaggc
    40981 aggtcttcaa attccagctc aacaggcgtt gccactgaaa tcaggttttc agggcttaat
    41041 ttgaagcggt tcagggggtt gtgttttgcc gtgcggcaga attccaggta ttccgtgccg
    41101 aggcttgccg ggtcatcggt gacggccaga cccaccagat aacatttgcc ggtgttggca
    41161 aagttcggct gaatttccat tgaggtgtag accttctgcg cggccttgtt catcgcgata
    41221 aggtcatcgg tcggggtgat tttcgcaaac agcgcccatt tgcctttcag cgccgaatca
    41281 tcgtcaatct tttcggcctt cagttcggcc acatcgccat aacgcttaaa aataccgtca
    41341 ggcaggatgc cgcgcagatg ttccaggtta atgcggcaac catagacacg cgggtcaaag
    41401 gtttcggcca tttcctgaat atcctgcgca ctgatgacac gcccgtcaca ggtgtcaccc
    41461 tcaacgccga tacgaaagaa ttttgagact ttttttgcca ttgtcaggag tcctgaatag
    41521 tgattagagg agtcacatgt cggcatcagt ttcccgacga tgcgcatcct ccgccatcag
    41581 tcccggatgg tttatcactg acacaacagc acattagcga atcgcggggc gcgactcagt
    41641 agccttgccg tgtattcatc acggcgaggt attcatgacc atcaccacag acaccactct
    41701 tttacacgac ccgcgtcgtc aggcggcgct gctgtactgg caggggtttt ccgtgccgca
    41761 gattgccgcc atgttgcaga tgaaacgccc gacggtgcag agctggaaac agcgcgacgg
    41821 ctgggacagt gttgccccca tcagccgtgt cgaaatgagt ctggaagcgc ggctgaccca
    41881 gctcatcatc aaaccgcaga aaaccggcgg tgacttcaag gaaattgacc tgctcggacg
    41941 ccagattgaa cgactggcac gggtaaaccg ctacagccag accggcaacg aggcagacct
    42001 taatccgaac atcgctaacc gcaacaaagg cgggcgtcgc aaaccgaaaa agaatttttt
    42061 cagtgacgag gccatcgaaa agctggagca gattttcttt gagcagtctt tcgactatca
    42121 gttgcactgg tatcgtgccg ggcttgagca ccgcatccgc gatatcctga aatcccgcca
    42181 gattggcgcg acgttttatt tttcccgcga ggcgctgctg cgcgccctga aaaccggcca
    42241 taaccagatt tttctgtcgg ccagtaaaac gcaggcgtat gtgttccgtg aatacatcat
    42301 cgcctttgct cgtctggttg acgttgacct gaccggtgac ccgattgtcc tgggcaataa
    42361 cggcgcaaaa ctgatttttc tcggcaccaa ctccaacacc gcacagagcc ataacggcga
    42421 cctgtacgtc gacgagattt tctggatccc gaattttcag gtactgcgta aggtggcatc
    42481 aggtatggcc tcacagagtc acctgcgctc gacctatttc tccaccccgt ccacgctggc
    42541 gcacgacgcc tacccgttct ggtcgggtga actgttcaac cggggacgcg ccagcgccgc
    42601 cgaacgtgtg gaaatcgacg tcagtcataa cgcccttgcc ggtggtcttc tctgtgcgga
    42661 cggccagtgg cggcagattg tcaccattga ggacgccctg aaaggcggct gcacattgtt
    42721 cgacattgag cagctcaaac gcgaaaacag cgccgacgat tttaaaaacc tgttcatgtg
    42781 tgaatttgtt gacgacaagg tatcggtgtt cccgttcgag gagctgcaac gctgcatggt
    42841 cgacacgctg gaagaatggg aagactacgc gccgtttgcc gcaaatccgt tcggctcccg
    42901 cccggtatgg attggttacg acccgtcaca ccgtggcgac agcgccggat gcgtggtgct
    42961 ggcaccgccg gtggtggccg gtggcaaatt cagaatactt gagcgtcacc agtggaaagg
    43021 catggacttt gccacccagg cggagtccat ccgcaaactc accgaaaaat acaacgtcga
    43081 atacatcggt attgatgcca ccggcctcgg tgtcggcgtg ttccagctcg tgcgctcgtt
    43141 ctatcccgcc gcgcgcgata tccgctacac gccggaaatg aaaaccgcaa tggtgctcaa
    43201 ggccaaagac gtcatccgcc gtggctgtct ggaatatgac gtcagcgcca ccgacatcac
    43261 cagctcgttc atggctatcc gcaagaccat gaccagcagc ggacgcagtg ccacctatga
    43321 ggccagccgc agcgaggaag ccagccacgc cgacctcgcc tgggcaacca tgcacgccct
    43381 gttaaatgag ccactcaccg ccggtatcag cactccgctg acatccacca ttctggagtt
    43441 ttactgatga gcaagaaaaa agggaaaaca ccgcgacctg cggcaaaaac aatcaccgcc
    43501 agcgccccga aaatggaagc attcaccttt ggtgagccgg tgccggtact cgaccgccgt
    43561 gacattctgg attacgtcga gtgcatcagt aacggcagat ggtatgagcc gccggtcagc
    43621 tttaccggtc tggcaaaaag cctgcgtgct gccgtgcatc acagctcacc gatttacgtc
    43681 aaacgtaata ttctggcttc aacgtttatc ccgcacccgt ggctttccca gcaggatttc
    43741 agccgttttg tgctggattt tctggtgttc ggtaatgcgt ttctggaaaa gcgttacagc
    43801 accaccggta aggtcatcag actggaaacc tcaccggcca aatatacccg tcgtggcgtg
    43861 gaggaggatg tttactggtg ggtgccgtcc ttcaacgagc cgacagcctt cgcgcccggc
    43921 tccgtgtttc acctgctgga gcctgatatt aatcaggagc tgtacggcct gccggaatat
    43981 ctcagcgccc ttaactctgc atggctgaat gagtcggcca cgctgttccg ccgcaagtat
    44041 tacgaaaacg gcgcacatgc cggatacatc atgtacgtca ccgatgccgt gcaggatcgc
    44101 aacgatatcg aaatgcttcg cgaaaacatg gttaagtcga aaggccgcaa taactttaaa
    44161 aatctgtttc tctatgcccc acaggggaaa gccgacggca ttaaaattat cccgctcagt
    44221 gaagtggcaa cgaaggacga tttttttaat atcaaaaaag ccagcgccgc tgacctgctg
    44281 gacgcgcacc gcatcccctt tcagttgatg gggggcaagc cggagaacgt cgggtcgctg
    44341 ggtgatattg agaaagtggc aaaggtcttt gtccgcaatg agcttatccc gttacaggac
    44401 aggatccgcg agataaacgg ctggctcggt caggaggtca tccgctttaa aaactactca
    44461 ctggacactg acaacggctg aacatcgccg cctgcgggcg gcttttttac accccgccat
    44521 cacgccctca cacgctcatc accgcacaaa aaatcccgca gacacaccaa cgcccaacgg
    44581 gcagactaag cgccgtcacg acgcgctgag acgctggaaa ataaaatcag caccaccgcc
    44641 agcgcgcagt gctttccccg cctcgcccgc ccgcttcatg gggcggtttt aatgcagttg
    44701 catgaacact ccaagagcac gccagcgcta gtgataacta acaataaaag cgacttctga
    44761 cgcatgcaaa tccatgcaca tgatggcatt tttcatgatc tatgcggtat gctatccgct
    44821 tcaattttta acacttgtta agggaactag acatggacat tattgattta aaatctcaac
    44881 tagaaaatga agataatgcc aagaaaagag cagagttgct tgctcagcta gaggtgataa
    44941 agcaaaacgc tgaagagcaa attcgtgctg agcaaaagga tgtggatttt gagaccaaag
    45001 aatttaccgt tgagttatta gttaataagt atcataacgg attagaggat gatactaacg
    45061 aactctttgt tcctgattat caaagggatt ttgtttggag tgaaaaaaga caatcaaaat
    45121 tgatagagtc tttgattttg ggttttccta taccatatat atttatagca gatgttctct
    45181 ccgaagatcc cgatttagat gggcgaattg agattgttga tggctcacaa cgagttagga
    45241 ctatacacgc ttttatccaa aatgaattag ttttacaaga cctgaaatct ttagacgtcc
    45301 ttaatggttt caaattccaa gatttaccat tatcacgtca acgtcgtttc atgagaattc
    45361 ctgtccgcgt tatagaattg agttctaaat gcaatgaaga aacccgtaga gatctttttg
    45421 agcgtattaa ctctggcagc gatatattga aagatatgga agtccgtaaa ggatcagaac
    45481 ttggttcaac aaatttatat acacaggtta ttaagccatg ctcaacaata cctaaattta
    45541 aagaattagc acctttatct cagactaaag aaaaacgaga tgaacggtta gaatttacat
    45601 taagattctt tgcctacgta gataagtaca agaactttga tcattccgta cgggattttc
    45661 taaatgacta tatgtcagaa aatcgagata tagacgaaac taaacaaaac tcaatgaaac
    45721 aagagtttgc taatgttcta tcttttgttg aaaactattt cccagcagga ttcaagaaaa
    45781 ctattactgc aaaatctaca cctcgagtta gattcgaatc tctcgctgtt ggcgcagcac
    45841 ttgcattacg tgaagtccct gaccttgttc ccaagaactt agattggcta agctcagaag
    45901 aattcaaacg attgaccact tccgacggag ctaatagtcg cgtgaaggta attgaacgaa
    45961 ttgaatatgt tcgggataaa ttattagagg attaacatgg atcgttttcg tgaggatttt
    46021 gacgaaaggt caggagagat cttggcctat ttggatcttt tgaaatttat tgagtatgcc
    46081 ggtgcagaac tgatatcagc tgatgataaa gaacataagt tctccattac tgctcaatca
    46141 cgaaaaactc taaagggagc agtatatata ctgttatata acctcattga atcaacaatg
    46201 agagaagcta tctgccttat tcatgagact atttatgata gaaacgtaga atttgacaaa
    46261 ctcaggaaaa atctcaggtc agaaatatta aaaagattaa aaaatgaatc tgtaaatgtt
    46321 gaaagtttag ttaatcacct taccaaaggt atatcttgcg gtatatctta tggcacgttc
    46381 aacaaaaaga aaatattctc aggaaatatt gatagagagg aaatcaaaga aaaatcgaaa
    46441 atatatggtt tttctacaca ttccgattac actcacacta aacatggtga aaaattagcc
    46501 actgttaaac aacatcgtaa tgatttatct catggcaatg tatcatttgc tgagattgga
    46561 aaaaatgttt catatcagga cttagagaat atttcattag aagtaatcgc ctatcttgat
    46621 gctatcgtga ataatataga gcactatata aataataatg aatatttaga acaatagtta
    46681 taaggcctgt aaagcaggcc attttatcat tctaaatggt tcttgattga ttgcccaatt
    46741 gcttttccta gttccacagg aacagcatta cctatcattt tcccaatact tcttataaca
    46801 aacttgtttt tgtcttctac aaaaatataa tccatcggga aagtttgcaa taaagcagcc
    46861 tctctaagag aaatcgctct atgctgttct ggatgtccaa aacgcccatt cccgaaacca
    46921 taacaaagag ttgtaattgt tggacttggc ttatcccaag acatgcgccc atatacacta
    46981 gcataccctt taccgctgga tttcatatga caagcggcaa ccaactcttt cggccagtcc
    47041 ttccatgtac caccaggaac agagtgtata attctttttt tattaatcgg atttaattta
    47101 ctggctctat gtaatatatc atttggatct gtttggccat cttctaatgg aggaagattt
    47161 cctattgcat ctctaactgt tttcaaaata acatcgtcac gttttactaa ctcaatcgcc
    47221 cccaattttg aggccaacaa tacatgacgg gaacggtttt gtgggattcc atattcgaca
    47281 caatcaatct tagacgc
//
LOCUS       DAZPLI010000014        46736 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZPLI010000014 DAZPLI010000000
VERSION     DAZPLI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.014"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(17..868)
                     /gene="pdxK"
                     /locus_tag="ACHUU3_000754"
                     /gene_synonym="thiJ"
     CDS             complement(17..868)
                     /gene="pdxK"
                     /locus_tag="ACHUU3_000754"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR2080916.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            973..1341
                     /locus_tag="ACHUU3_000755"
     CDS             973..1341
                     /locus_tag="ACHUU3_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR2080917.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(1344..2255)
                     /gene="cysM"
                     /locus_tag="ACHUU3_000756"
     CDS             complement(1344..2255)
                     /gene="cysM"
                     /locus_tag="ACHUU3_000756"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR2080918.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(2389..3486)
                     /gene="cysA"
                     /locus_tag="ACHUU3_000757"
     CDS             complement(2389..3486)
                     /gene="cysA"
                     /locus_tag="ACHUU3_000757"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR2080919.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(3476..4351)
                     /gene="cysW"
                     /locus_tag="ACHUU3_000758"
     CDS             complement(3476..4351)
                     /gene="cysW"
                     /locus_tag="ACHUU3_000758"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR2080920.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(4351..5184)
                     /gene="cysT"
                     /locus_tag="ACHUU3_000759"
     CDS             complement(4351..5184)
                     /gene="cysT"
                     /locus_tag="ACHUU3_000759"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR2080921.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(5184..6200)
                     /gene="cysP"
                     /locus_tag="ACHUU3_000760"
     CDS             complement(5184..6200)
                     /gene="cysP"
                     /locus_tag="ACHUU3_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR2080922.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(6371..7162)
                     /gene="ucpA"
                     /locus_tag="ACHUU3_000761"
     CDS             complement(6371..7162)
                     /gene="ucpA"
                     /locus_tag="ACHUU3_000761"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR2080923.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(7291..8148)
                     /gene="murR"
                     /locus_tag="ACHUU3_000762"
     CDS             complement(7291..8148)
                     /gene="murR"
                     /locus_tag="ACHUU3_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFR2080924.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            8312..9208
                     /gene="murQ"
                     /locus_tag="ACHUU3_000763"
     CDS             8312..9208
                     /gene="murQ"
                     /locus_tag="ACHUU3_000763"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR2080925.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            9212..10636
                     /gene="murP"
                     /locus_tag="ACHUU3_000764"
                     /pseudo
     CDS             9212..10636
                     /gene="murP"
                     /locus_tag="ACHUU3_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            10641..11945
                     /gene="pbp4b"
                     /locus_tag="ACHUU3_000765"
     CDS             10641..11945
                     /gene="pbp4b"
                     /locus_tag="ACHUU3_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFR2080926.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(12003..12902)
                     /gene="yfeX"
                     /locus_tag="ACHUU3_000766"
     CDS             complement(12003..12902)
                     /gene="yfeX"
                     /locus_tag="ACHUU3_000766"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR2080927.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(12998..13573)
                     /locus_tag="ACHUU3_000767"
     CDS             complement(12998..13573)
                     /locus_tag="ACHUU3_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR2080928.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(13634..14083)
                     /locus_tag="ACHUU3_000768"
     CDS             complement(13634..14083)
                     /locus_tag="ACHUU3_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR2080929.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(14070..14495)
                     /locus_tag="ACHUU3_000769"
     CDS             complement(14070..14495)
                     /locus_tag="ACHUU3_000769"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR2080930.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            14709..15578
                     /gene="amiA"
                     /locus_tag="ACHUU3_000770"
     CDS             14709..15578
                     /gene="amiA"
                     /locus_tag="ACHUU3_000770"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR2080931.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLPGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            15582..16481
                     /gene="hemF"
                     /locus_tag="ACHUU3_000771"
     CDS             15582..16481
                     /gene="hemF"
                     /locus_tag="ACHUU3_000771"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR2080932.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(16487..17539)
                     /gene="eutR"
                     /locus_tag="ACHUU3_000772"
     CDS             complement(16487..17539)
                     /gene="eutR"
                     /locus_tag="ACHUU3_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFR2080933.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(17585..18085)
                     /gene="eutK"
                     /locus_tag="ACHUU3_000773"
     CDS             complement(17585..18085)
                     /gene="eutK"
                     /locus_tag="ACHUU3_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR2080934.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(18098..18757)
                     /gene="eutL"
                     /locus_tag="ACHUU3_000774"
     CDS             complement(18098..18757)
                     /gene="eutL"
                     /locus_tag="ACHUU3_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR2080935.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(18767..19654)
                     /gene="eutC"
                     /locus_tag="ACHUU3_000775"
     CDS             complement(18767..19654)
                     /gene="eutC"
                     /locus_tag="ACHUU3_000775"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFR2080936.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(19675..21036)
                     /gene="eutB"
                     /locus_tag="ACHUU3_000776"
     CDS             complement(19675..21036)
                     /gene="eutB"
                     /locus_tag="ACHUU3_000776"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFR2080937.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNRSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(21048..22451)
                     /gene="eutA"
                     /locus_tag="ACHUU3_000777"
     CDS             complement(21048..22451)
                     /gene="eutA"
                     /locus_tag="ACHUU3_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR2080938.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(22448..23674)
                     /gene="eutH"
                     /locus_tag="ACHUU3_000778"
     CDS             complement(22448..23674)
                     /gene="eutH"
                     /locus_tag="ACHUU3_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFR2080939.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKTGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            complement(23991..25178)
                     /gene="eutG"
                     /locus_tag="ACHUU3_000779"
     CDS             complement(23991..25178)
                     /gene="eutG"
                     /locus_tag="ACHUU3_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFR2080940.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(25168..26004)
                     /gene="eutJ"
                     /locus_tag="ACHUU3_000780"
     CDS             complement(25168..26004)
                     /gene="eutJ"
                     /locus_tag="ACHUU3_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFR2080941.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(26015..27418)
                     /locus_tag="ACHUU3_000781"
     CDS             complement(26015..27418)
                     /locus_tag="ACHUU3_000781"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFR2080942.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(27430..27717)
                     /gene="eutN"
                     /locus_tag="ACHUU3_000782"
     CDS             complement(27430..27717)
                     /gene="eutN"
                     /locus_tag="ACHUU3_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFR2080943.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(27824..28117)
                     /gene="eutM"
                     /locus_tag="ACHUU3_000783"
     CDS             complement(27824..28117)
                     /gene="eutM"
                     /locus_tag="ACHUU3_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFR2080944.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(28156..29172)
                     /gene="pta"
                     /locus_tag="ACHUU3_000784"
     CDS             complement(28156..29172)
                     /gene="pta"
                     /locus_tag="ACHUU3_000784"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR2080945.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(29169..29972)
                     /gene="eutT"
                     /locus_tag="ACHUU3_000785"
     CDS             complement(29169..29972)
                     /gene="eutT"
                     /locus_tag="ACHUU3_000785"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFR2080946.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            complement(29969..30670)
                     /gene="eutQ"
                     /locus_tag="ACHUU3_000786"
     CDS             complement(29969..30670)
                     /gene="eutQ"
                     /locus_tag="ACHUU3_000786"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFR2080947.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(30645..31124)
                     /gene="eutP"
                     /locus_tag="ACHUU3_000787"
     CDS             complement(30645..31124)
                     /gene="eutP"
                     /locus_tag="ACHUU3_000787"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR2080948.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(31137..31472)
                     /gene="eutS"
                     /locus_tag="ACHUU3_000788"
     CDS             complement(31137..31472)
                     /gene="eutS"
                     /locus_tag="ACHUU3_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR2080949.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(31766..34045)
                     /gene="maeB"
                     /locus_tag="ACHUU3_000789"
     CDS             complement(31766..34045)
                     /gene="maeB"
                     /locus_tag="ACHUU3_000789"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR2080950.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            34334..35284
                     /gene="tal"
                     /locus_tag="ACHUU3_000790"
     CDS             34334..35284
                     /gene="tal"
                     /locus_tag="ACHUU3_000790"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR2080951.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            35304..37307
                     /gene="tkt"
                     /locus_tag="ACHUU3_000791"
     CDS             35304..37307
                     /gene="tkt"
                     /locus_tag="ACHUU3_000791"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR2080952.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(37403..38446)
                     /locus_tag="ACHUU3_000792"
     CDS             complement(37403..38446)
                     /locus_tag="ACHUU3_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR2080953.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(38572..39147)
                     /gene="nudK"
                     /locus_tag="ACHUU3_000793"
                     /pseudo
     CDS             complement(38572..39147)
                     /gene="nudK"
                     /locus_tag="ACHUU3_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
     gene            complement(39215..41194)
                     /gene="aegA"
                     /locus_tag="ACHUU3_000794"
     CDS             complement(39215..41194)
                     /gene="aegA"
                     /locus_tag="ACHUU3_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR2080954.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            41400..43100
                     /gene="narQ"
                     /locus_tag="ACHUU3_000795"
     CDS             41400..43100
                     /gene="narQ"
                     /locus_tag="ACHUU3_000795"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFR2080955.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            43264..46377
                     /gene="acrD"
                     /locus_tag="ACHUU3_000796"
     CDS             43264..46377
                     /gene="acrD"
                     /locus_tag="ACHUU3_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR2080956.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(46476..46535)
                     /gene="ypfM"
                     /locus_tag="ACHUU3_000797"
     CDS             complement(46476..46535)
                     /gene="ypfM"
                     /locus_tag="ACHUU3_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR2080957.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        10689 a        13151 c        12112 g        10784 t
ORIGIN      
        1 ccatcggcgc cattttttat gcttccgcca gcggcggcaa aatcaattca tcgctctcat
       61 gctgctgggt gtagcgcatc acttccagta cgcgcaaccc cgcacggtgc accgcatcgt
      121 ttaacgcctt ccctttcagc aaaccactga taagctgagc acaaaacagg tcgccagtcc
      181 ctttcaggtc ggtttttacc cgtgaatggg aaatgacatt cacgctgtcg gcactgacca
      241 ccaccacctg catctcctga ttttcttcat taccggaggc gctggtaatc accacccatt
      301 ttaatgtgtc tgaaagcaga ctttttgcgg cagcaatggc actgtcgaga tcgcggcaat
      361 ttttaccggt caggatttcc agctcaaaga tattgggggt aattccttgc gccagcggca
      421 gtaaatattg tcgatacgcc tcgggaaggt caggtttgac ataaattccg ctatcaatat
      481 cgccaatcac cggatcgacc atgatcaata ggtcaggatg gtctttgcgt agcgcagtca
      541 gccactcggc aaggattttg atttgcgatg ccgttcccat atagcccgtg gttacagcac
      601 gaagttggcg cagcgcatca cgctcctgaa gcgcacgcaa atagccgcta aaccattcgt
      661 ccggaatcgc accaccgtag aaagtgtcat aatgcggcgt attgctcagc aataccgtcg
      721 gcacggcaaa gacattcaag ccgttctgtt ttatagcagg caccgcaatg ctgttgccta
      781 cgctgccgta cactacctgc gactgcacgg cgacgatatc cgcctgcagc gccctactct
      841 tatcgttaaa caacaacaaa ctactcattt aattttttct ccttgccgat gatcctcatc
      901 gtattccaac cgaaacttta cctgattctg gcagtcaaat cggctatcac aaaacaagga
      961 taaggtaatt caatgaagaa aatcatttgt ctggtcatta cactactaat gacactcccc
     1021 gcttacgcga agttaactgt tcatgaagaa gcccgcatca acgccatgct ggagggatta
     1081 gcacagaaaa aggatttgat atttgtgcgc aacggtgatg aacatacctg cgatgaagcg
     1141 gtttctcatc tgcgtctgaa gctcggcaat acccgtaacc gcattgacac tgccgagcag
     1201 tttattgata aggttgcttc gtcgtcatcg attaccggga agccgtatat tgtgaagatc
     1261 cccggtaaga gcgatgagaa cgcacagcct tttttacatg ccttaattgc gcagacggat
     1321 aaaacggtgc ctgcgcaata atcttaaatc cccgccccct gactaaaatg ctcttcccca
     1381 aacaccccgg tggaaaggta gcgatcgcca cgatcgcaga tgatcgctac caccaccgca
     1441 ccagggttag cttttgccac ccgcagtgct ccggcaaccg cgccgccgga gctgacgcca
     1501 cagaatattc cttcccgcac tgccagttcg cgcatggtgt tttctgcatc gcgctgatga
     1561 atatccagca cctcatccac cagagaagcg ttgaaaatcc ccggcagata ttccgcaggc
     1621 cagcggcgaa tgccgggaat actgctgcct tcttccggct gcaggccaac aatcgttact
     1681 ggtttggatt gttcgcgcat aaagcgtgaa acgccggtga tagtgccggt tgttcccatg
     1741 ctggagacaa aatgggtaat gcgcccgccg gtttgctgcc agatttccgg cccggtagtg
     1801 gtgtaatggg cgtaagggtt atcgggatta ttgaactgat cgagcagctt tccttcgcca
     1861 cgattcgcca tctccagcgc cagatcgcgc gcaccttcca tgccctgctc tttggtgaca
     1921 agaatcagtt ccgcaccata agcacgcatc gccgcacggc gttcctggct catgttgtcg
     1981 ggcatcagca atttcatgcg atagcctttc agcgcggcaa tcattgccag cgcaatgccg
     2041 gtgttaccac tggtggcttc gattaagaca tcacccggtt taatttcccc gcgcttttcc
     2101 gcctcgacga tcatcgaaag tgccgcacga tctttcaccg aacctgccgg gttattgcct
     2161 tccagtttta accacacttc actgccgtta tccggcccca ttcgctgcaa cttcaccaga
     2221 ggcgtattgc ctattgtttg ttctaatgta ctcacgatct ctatccatac gtggtgttgc
     2281 ctgatgcgac gcttgcgcgt cttatcaggt ctacaggtta caaaccttgc cattaaaaag
     2341 cccggatcgc gggaagcgcc tccgggcgtt taacattcac tcaacctatc aggcgctttg
     2401 tgcgagagca agttcctcat cgcgggtttc gatacgctcg tcgccgttat acagccgcgc
     2461 atgttgcaga ccaacgaata aacgctcgcc acgctgcggg gcatcgtcgc catgcatcac
     2521 gaccgtcagc ggttcgttgt accaccccag cggctgcacc actaattggg tgtagtgacc
     2581 tttcgggctg gcttccagta cctgtaccgg cagcggcgaa tcgagactgg tacggcggct
     2641 gatatccact tcccacgggc gcaggaagag atccaccggc ccctgataag caggtgtgta
     2701 gcccagcggc cagcgatgtg cgccaacatg gaactgcccg ccgcgaatgg ttccctgcag
     2761 gcggttcact tcgcccataa attcgagcac aaaacgggtc gctggttcgc gccatacctg
     2821 ctccggcgca tcagcctgtt caatattgcc ctggctcatc accactacac gatcagctac
     2881 ttccgtcgct tcttcctggt cgtgagtcac gaacacgctg gtgaatttca gctcttcatg
     2941 cagttgacgc agccagcgac gcagctcttt acgcacctgc gcatccagcg cgccaaacgg
     3001 ttcatcaagc agcagaattt gcggttctac agccagcgcg cgcgccagcg ccacgcgctg
     3061 tttctggccg ccggaaagct gtgccggata acgatccgcc agatgagcaa gctgcaccat
     3121 ctccagcaat tttgtcactt tcgctttgat ggctgcggca ttcgggcgat cgcgacgcgg
     3181 cagcaccgtc aggccaaaag cgatattgtc gaataccgtc atatggcgga acagcgcgta
     3241 atgctggaac acgaaaccga ccttgcgatc gcgcgcgtgc aggcggctga cgtcggtgcc
     3301 gtggaagcga atatgcccgc tggtttgatg ctccagcccg gcgataatgc gcagcagcgt
     3361 ggttttcccg gaaccggacg gccccagcaa cgcgaccatc tgacctgaag gaatatccag
     3421 tgagatatcg ttcagcacct gggtgcgacc aaacgacttc ttaatattgg caatctcaat
     3481 gctcatgatg ttcctcctgc tgtgcgcgtt tttcctgatt ctccaggcgc cactgcaaca
     3541 tactttttaa aaacagggtg ataatcgcca tcagcgttaa cagtgccgca gcggtaaagg
     3601 agccgacggt gttgtagtcc tgctccagca attcaatctg taacggcagc gacagggttt
     3661 cgccgcgaat cgagccggaa accaccgaca ccgcgccaaa ctcgccaatt gcgcgggcgt
     3721 tggtcaacac cacgccataa agcagcgccc agcgaatgtt cggcaatgtg acgcggcgaa
     3781 acatctgcca gccagacgcg ccaagcaaaa tcgccgcttc gtcttcctgg ctgccctggc
     3841 ttaacatcac cggcaccagc tcgcgcacca caaacggaca cgtcacaaag atggtgacca
     3901 gcaccattcc gggccaggag aacataattt gcagattatg ttcatccagc cagccgccca
     3961 gcgggccgtt agagccgtag aacagcagat acaccagacc ggcaaccacc ggcgatacgg
     4021 caaacggaat gtccagtagc gtcagcagta actggcgtcc agggaagtta aagcgcgtca
     4081 ccagccaggc cagcagaatg ccgaacacca ggtttaccgg tacggcaatc agcgcgatca
     4141 tcaccgtcag ccagatggcg tgcagcatgt ccggatcggc cagattctgt aaaaccggca
     4201 tcagcccttt gctgaacgcc tgtacgaaga tataaatcat cggcaccagc aggatgaacg
     4261 ccgataccag catcccgatg ccaatcagaa accatttgcc ccagttaatc gggcgcgcgt
     4321 cataacgctt caattgggta acttccgcca ttaatgacct accacacgcc gaccaaagcg
     4381 actttgcaga gtgttaattg agaacagcag cagcagagat gccgcgagga tcaccgaagc
     4441 aatcgcgctc gctgccgggt aatcaaactc ctgtaagcgc acaaaaatca tcagcgacgt
     4501 cacttccgtc ttccacgcga tatttccggc gataaaaatc accgcgccaa attcaccaag
     4561 actacgggta aacgacagcg ccacgcccgc caccagcgcc ggagaaagtt ccggcagcac
     4621 cactttgcag aaactctgcc agcgcgttgc gccaagcgtt tctgccgctt cttcatattc
     4681 cgggcctaac tcttccagca ccggctgcac ggtacgtacc acaaacggaa tgctggtaaa
     4741 ggccatcgcc accgcaatcc ccagccaggt ataggtgact ttgatatcaa acttcgccag
     4801 ccattcaccg taaaaaccgt ttacagaaaa gagcgaggct agcgttaaac cggcgactgc
     4861 cgttggcagc gcaaagggta aatccatcag cgcatcaagc agcgtgcggc ccggaaagcg
     4921 atagcgggtt aagatccacg ccatcagcag gccaaaaacg ccgttaaaaa tcgatgccac
     4981 aaacgctgac agcagcgtta ctttgtaggc cgctactacc tgcgggttgg tgatcacctc
     5041 ccagtactgc gcccagctca tctgagccag ctgcatcacc agcgcggaga gcggcagcag
     5101 caaaatcagg cacacaaaca gcagactggt gccgaggctt aaggtaaagc ccggcagcac
     5161 gcgtcttgat gaaactgcaa acattaatta cgccccgccg ctaacagctt gtctaactcg
     5221 ccgccgctgg tgaagtgggt tttcatcact tccggccagg agccaaattt gtcttccacg
     5281 cggaacagct cggtctgcgg gaatttgtct ttcagtttat ccatgacttc cgggttattc
     5341 acgcggtagt aatagtcggt gatgatggtt tgcgcctgcg ggctgtagag ccagttaagg
     5401 taggctttgg cggctttttc cgtaccgttg gcctgcacgt ttttatcaac ccacgccacc
     5461 gggaattccg ccagaatgtt ggttttcgga atcaccactt caaaaccctg cgcttcatac
     5521 tgtttacgga tgttgttcac ttccgactcg aagctgatca gcacatcgcc caggccgcgc
     5581 tcggcaaaag tggtggtcgc gccacgaccg ccagtatcga acacttcaac gtttttcagg
     5641 aactgggtca taaactgttc ggttttggct ttatcgccac cgtcagcttt atccgctgcg
     5701 ccccatgccg ccagataggt gtaacgcgcg ttacccgacg ttttcgggtt cgggaaaatc
     5761 agcttcacat cggagcgcac caggtcgttc cagtcgtgga tattcttcgg gttgccctta
     5821 cgcaccagga agcccatggt ggagtagaat ggcgagctat tatttggcag gcgcgtttgc
     5881 cagtccgccg ggatcagctt gcctttgtca tgcaggattt gtacgtcagt cacctggtta
     5941 taagtgacaa cgtctgcttt taagccctgc aaaattgcca gcgcctgttt tgatgacccg
     6001 gcatgagatt gttttattgt cagtttgtcg ccgccgtttt ctttggtcca ttgttgctca
     6061 aacggcgggt tcagggcggc aaacagctcg cgggagacgt cataagaact gttcagcagt
     6121 tccgttgcct gtacatggcc cgccagcagc agagaagcga ccagcgcgag tgagttcttt
     6181 ttcagtaagt taacggccat tgcgcaccct tataaattta atgactttct aatagccatc
     6241 atatttataa cggacgtgaa aggagtaacg gttttatata ccgtttggtg atttggaagt
     6301 tgaaaaggga ataagaaccg ggtttaaaac gaaatcctcc ccacaaatgc agggaggaaa
     6361 cagaggtgaa tcagataccg acgctaaccg tctccggcag tgtgctgccg ccatcaatca
     6421 cattctgtgt accggttaaa tagctggatt catccgatgc gaggaaggcc gccagttcgc
     6481 cgacttccag cggatcggcg aggcgacgca gcgggattgc tttcgccatt tcagtcagca
     6541 ccgactctgg atcttccggg ttcgactggc gggcaatgct ttccgccatt ggtgtgcgca
     6601 cgtatcccgg gcaaatggcg ttaacgcgaa taccagactg cgcgtactcc accgccagcg
     6661 attttgtcag gccaacaatc gccgctttcg ttaaggcgta cgccgtttcg ccaggatcgg
     6721 ccaccatatc accagtgact gaagacatca tcacaatgcg accatctttg cgggcaatca
     6781 tctccggcag caccgccttc gtgacgttcc atacgccttt aatattgatg tcaatatgga
     6841 aatcgcgatc gtcatcgctc atatcgagga aactgcccag acgacaaacg cctgcgttat
     6901 tcaccaggat atcaatgcgc ccttcttttt ccttcgcgcg tttgatagct gcggctaccg
     6961 acgccgggtc acgcacatcg gcgacaaccg ccgtacagcg atgaccacga ccacacagtt
     7021 cgtccgccag cttttcgatc tcaggggaga tatccagcaa gattaggttt gcgccatgac
     7081 gcgcaaaagt tctggcaatt ccttcgccaa ttccctgcaa tgcgcccgta atcagtgctg
     7141 tcttgcccgt gagtttaccc attttaatgc tctccttgtt aatacagagc gttacactcc
     7201 attccctctg aaaatacagg ataaacaaaa tattgctgcg gatcagctca ctaattcagg
     7261 aaaatctcaa tccagtcgct gctttatgca tcatttcagg cgttgtgtta gctcactgct
     7321 gcgctgaatc attttcaatg actccacatc attgagttgc acaagaccaa caaacagtaa
     7381 atcggtaaca gaattttgcg ccgtgcgggt agacatcgac gagctacgcc attcggtttc
     7441 accggaaacg gtatcgagcg tgaaatgcgc cagccgccgc agcggagagt cagccagaga
     7501 agtaatggca atcaccgttg ccccctgctt tcgtgccgct tctgcgcaca agacaatctc
     7561 ttttttactg ccgctgtaag agatagcgat ttgtacatcg ccttttttca acgcctggga
     7621 aacggtggct tgtacgtgtg tgtcggcttc ataggccacg cgatagccaa ttttcatcag
     7681 tttgaacgac agatcgcgcc ccaccagcgc cgacccgccc aggcccgtta tctggataaa
     7741 cggtgcttta ctgatgacct caatgatttt ttgcagccga gcgtagtcga acaacgcaca
     7801 ggtttgctcc agcgccagct ctttttcgcg attcagtttg cgggcaatca cttccagcga
     7861 atcatcactg gtaatcgaac tgtgcaggtg cagcgccgtg gcattcgttt tctcccggct
     7921 ggcactgtat tcgccaatta acgccatacg caattcagta aatccttgcg cgccgagctt
     7981 ctgggcaaat ttcacaatgc tcgactgact gatgccgagc tgtttcgcca tcttgcgcga
     8041 agagacggat ttcagctcac tcacgttggc ccgcagaaag tcagcaattt tttgctcgtt
     8101 ctcggtaaat tcactttccg cattgcgtat tttcgtcagg tacaacatct tccactctcc
     8161 cgggctggca gcaaaaattc cgcttaagtt cttatgacgc tcttcacact ctgcaagtgt
     8221 agactcaatg attcctttaa gtctgtcaat tcgggatata tgattccata catctgcaat
     8281 tatttgatcg taaatagtaa ggtcaccacc gatgcaactt gaaaagatga ttactgaagg
     8341 ctcgaacacc gcctcggctg aaattgaccg cgtatcgacg ctggaaatgt gccggattat
     8401 caacgatgaa gataaaaccg taccgcttgc cgttgagcgc gtcctgccgg atatcgccgc
     8461 ggcgatcgat gttatccacg cccaggtaag cggcggcggt cgtctgattt atctcggtgc
     8521 gggaacatcc ggtcgtctgg ggattctgga tgccagcgaa tgtccgccca cctacggcgt
     8581 gaaaccgggt ctggtggttg gtttgattgc tggcggcgaa tatgccattc agcacgcggt
     8641 ggaaggcacg gaagatagcc gagaaggcgg cattaacgat ctgaaaaata taaatttaac
     8701 ggcacaggat gtggtggtcg gtattgccgc cagcggtcgt acgccgtatg tgattgccgg
     8761 actggaatac gcccgccagc tcggttgccg cacagtggga atttcctgta atccggggag
     8821 tgccgtttcc accaccgccg agtttgccat tacaccgatt gtaggtgccg aagtggtcac
     8881 tggttcttcg cgaatgaaag caggcacagc gcagaaactg gtgctcaata tgctttccac
     8941 cgggctgatg attaaatccg gcaaagtgtt cggcaacctg atggtcgatg tggtcgccac
     9001 caacgaaaaa ctgcatgtgc gccaggtcaa tatcgttaaa aacgccaccg gatgtaatgc
     9061 cgaacaagcg gaagcggcgt taattgcctg cgagcgcaac tgtaaaacgg ccattgtgat
     9121 ggtgctgaaa aatctcgatg ccgcagaagc taaaaaacgc ctggatcaac acggcggctt
     9181 tattcgtcag gttttagaca aggaataacc catggccaaa gagatcagca gtgaacttct
     9241 gaacaccatt cttacccgtg tcggcggact gggaaatatc gccagttgtg gtaactgtat
     9301 gacgcgcctg cgtctgggtg tacatgacag ttcactggtt gatcccaata tcaaaacgct
     9361 ggaaggcgtg aagggcgtca ttttgaccag cgaccaggtg caggtcgttt ttgggcctgg
     9421 taaagcgcat cgtgccgcaa aagcgatgag cgagctgctg ggcgaggcac cggtacagga
     9481 tgccgcagaa atcgccgccc agaacaaacg tcagttaaaa gccaaacaaa cctccggcgt
     9541 gcaacaattt ctcgccaaat tcgccaccat cttcacgccg ctgatccccg gttttattgc
     9601 cgccggtctg ctgctgggga tagcgacgtt aattgccacg gtgatgcacg ttccggcaga
     9661 tgctcagggc acgctacccg acgcgctgaa ttttatgaag gtgttcagca aaggtttgtt
     9721 cactttcctg gtgattctgg tgggctataa cgccgcccag gcatttggcg gcacgggcgt
     9781 caatggcgca attattgccg cactcttttt gctcggttat aaccccgctg caaccaccgg
     9841 ttactacgcc ggttttcacg atttctttgg tctgcccatc gatccgcgcg gcaatattat
     9901 cggcgtgctg attgccgcgt gggcctgcgc ccgcattgaa ggcatggtgc gccgctttat
     9961 gtcggacgat ctcgacatgc tgctgacctc gttaatcacc ctgctgatca ccgctacgct
    10021 tgcgtacctg atcattatgc cgctgggtgg ctggttattc gaaggtatgt catggctgtt
    10081 tatgcacctg aacagtaatc cgttcggttg tgcggtttta gccgggctgt tcctgatcgc
    10141 cgtggtattt ggcgtgcatc agggctttat tcctgtttac ctcgcgttaa tggacagcca
    10201 gggattcaac agcttatttc ccatcctttc aatggcaggc gcgggccagg tgggcgcggc
    10261 gctggcactc tactggcggg cgcaaccgca cagtgcgcta cgcagtcagg tacgcggggc
    10321 gattattccc ggcctgctgg gcgttggtga accgctgatt tacggtgtca ccctgccccg
    10381 catgaagccg tttgttaccg cctgtttagg cggcgcggcg ggcggtttgt ttatcggcct
    10441 gatagcctga tggggtctgc cgatgggctt aaacagcgcc tttgggccgt ctggtctggt
    10501 agcgctgccg ctgatgactt ccgcacaagg catccttccg gccatggcgg tttatgctgg
    10561 cgggattctg gtggcatggg tttgcgggtt tattttcacc acgctctttg gctgccgtaa
    10621 cgtcaatctg gactgatatc atgaaacgga caatgctcta tctttctctg ctggctgtta
    10681 gttgtagcgt cagcgccgca aaataccctg ttctgacaga aagctcgcca gagaaagcag
    10741 ggtttaacgt cgaacggctt aaccagatgg atcgctggat tagccagcaa attgatgtcg
    10801 gttatcccgg cgtaaacctg ctgatcatta aagataatca gattgtgtat cgcaaagcct
    10861 ggggagcggc gaaaaagtac gatggcagcg tgttgatgga acagcctgtc aaagccacca
    10921 ccgggacgct gtatgacctg gcctcaaaca ccaaaatgta cgccacgaac ttcgccctgc
    10981 aaaagctgat gagcgaaggc aaactgcatc ctgacgatcg gattgcgaaa tatattccgg
    11041 ggtttgccga tagtccaaat gacaccatta aaggcaaaaa caccctgcgg atttctgacc
    11101 tgctgcatca cagcggcggt ttcccggcag atccgcaata cccgaataaa gcggtcgcgg
    11161 gcgcgttata ttcccaggat aaaggccaga cgctggagat gatcaagcgc acgccgctgg
    11221 aatatcagcc cggcagcaaa catatctaca gcgatgtcga ttatatgctg cttggattta
    11281 tcgtcgagtc ggttaccggt cagccgcttg accgctatgt tgaagagtcg atttatcgcc
    11341 cgctcggcct gacacatacg gtatttaacc cgctgctgaa aggctttaaa ccgcaacaaa
    11401 ttgccgccac ggaattgaac ggcaataccc gcgatggcgt gatccatttc ccgaatatcc
    11461 gcacctccac tctctggggt caggtgcacg atgaaaaagc cttttattcg atgggcggcg
    11521 tttccgggca cgcaggtttg ttttccaata ccggcgatat tgcggtgtta atgcaaacga
    11581 tgctgaacgg cgggggctat ggcgatgtgc agctgttcag tgcggaaacg gtgaagatgt
    11641 tcaccaccag ctctaaggaa gatgccactt ttggcctcgg ctggcgcgtg aatggtaatg
    11701 ccaccatgac gccgacgttt ggcacgctgg caagcccgca aacctacggt cacactggct
    11761 ggaccgggac agtaaccgtt atcgatccgg tcaatcatat ggcgattgtg atgttaagca
    11821 acaagcccca ttcgccagtt gccgatccac aaaaaaatcc caatatgttc gaaagcggtc
    11881 agttaccgat tgcaacttat ggttgggtag ttgatcaggt gtatgcggcg ttgaagcaaa
    11941 agtaataaaa aggccagtca gttttgttac tggctggcct ttaatcatcg aatcagaaac
    12001 gcttacaacg ccattaactt gtccagcgac ggcgcgaagt aatagccgcc ggttaccggt
    12061 ttggtgaaac gcaacatcgc atcacgctta ccatcggtat cgccaaacat gctcagcagt
    12121 tgctgctcaa tgttatgcag acgcgcgcag taggcgcaga agtacagacc gtgagtgcca
    12181 ctggcggtgc cgtacggcag gctctggcga acaatcttca gccctttgcc atcttctttc
    12241 agatcaacgc gggtgaggtg agaggtttcc ggacgttcgt cgccgtcaat ctcttcgttg
    12301 gcctctttgg tgcgcccgat catcatctcc tgatcgtgaa cgctcatccg gttgagctgc
    12361 ttcaggttgt gctcccaacg ctgcacaaac acatagctgc cgccggcatc cacgccgtct
    12421 ttgataaccg ccacttcgcg acgcgtctct tcacccgccg ggttttctgt accgtcaaca
    12481 aagccgctca ggtcacgctc ttccacccaa cggaagccgt ggatctcttc tttcacttca
    12541 atgcagtcac caaaggcttc catcgccgcc tgggcgacag agaagtttac gtcgtgacgc
    12601 agagaaagaa tgtggatcaa cacatcgaac tgggtggtcg gcgcaaggcc tttaccgtag
    12661 cccggaaaat ctttcagctc ttctgcccca acgccgccgc tcagagcgcg ccaggtgttg
    12721 ttaccaaagg caaccaccgc accaagatgc gcgtccggga atttcgcttc aaaagttgcc
    12781 agtttgtcgg caaatgtttt actggccgca cgcagggcgt caacttcccc tttcacgttg
    12841 gcttcgatcc aaatcgccgc gcggcaatgt tctggcaaaa tgccactctg aacctgagac
    12901 attgttcctc ctgaaaataa gaatgccacg caatccgtgg cattgttgag cgctatttta
    12961 cgtgtttttt cgcggcggcg gtttgttcag acgcaaatta acgccgccag ataattttac
    13021 tgactttcca gtttttcagg gtatcgtccg aaggcattaa cccttcagga ccacgccatt
    13081 ccccgctgaa ctggtagcta atatgttgac tgccttcggc tttacattcg acggcacgat
    13141 tatcatcacc atcagctttt tggcaattgc caaatgcttt gctgtaaagg tcgctaaacg
    13201 gtgtaccgat tttaacaccg gtgtcagcag gaatatcgct atccagcacg tcaatgcgac
    13261 tgatcgtgcc ttgatcgccg ttaatcacca tcgccacgtt gtcgcctttc atcacttcaa
    13321 aaaagcgcac cacgttgccg ttcgcggttt tcattccgct gcgcaggcga taatcgccat
    13381 caagcgcatc agcaatggct tgttcttgca gtggtgtgga cgccgttaat tcacccacgc
    13441 cctgctcgct cactttggtg gacgatccaa accagttcca cgggttagcg gccgaccagt
    13501 taactgacga catcgtggaa cagccggtca gcatcaacgg catagcgcat aacattaaac
    13561 gcagcgattt catgtcactt cctttggtta ttcaataacg ttgcttggag tgcaaattca
    13621 ccaaaaagtg ccgttattct ttttcttcat taaaacaagc gcgtaaacgt cgattggtca
    13681 gcagccagat taacgccaca atatccgcca ctaccagcgc cagaccgata ccgctaacgg
    13741 attcaccgtt cagccacagc cacggttgcc agcaaagtaa aaccacctgc gccaacagca
    13801 acagaaaata gagcacatgc caagtgcgag gaaatgtagc ccgccgaccg ctcaacaaaa
    13861 acgccagcac cgccggaatg cctggaatca gccccagcca gaaattatcg tgatcgggat
    13921 aaaacagatt tagcagcgca gtaccctgct cgcgcgacgc accggcaatg acaaacagca
    13981 cccaggttcg cgcctgaagc aatagcacaa gccagaagag caagggtaaa cgcaggcgtc
    14041 cgtgcgcatc ataatggaca ggatggaact cagtactctt catcttcaat caaacgctta
    14101 cccagactca gcacgtcggc gtgttcatat cccaggcgtt catacattcc gagcaccatg
    14161 tcgttatctt ccggcacatt gatctgaatt ttcgggcagc cacgagcaat cagctttttc
    14221 tccagccgat taagcaacgc attggcaatc ccacgcccac gaaactctgg atgcacgcca
    14281 agataataag cagacccgcg atgcccgtca taaccgccca tcaccgttcc gaccacttca
    14341 ccgtttacct cagcgaccaa aaacaaactg acgtcatggt tcatcttacg ctcgatgtcc
    14401 atttccggat cgttccacgg acgcagcaag tcgcaacgct cccaaagggt gatgacctct
    14461 tcgaaatctt cctggcgaaa tacgcgtatc tccatggtat tcgttacctt tttgcgggtt
    14521 aaaaggctga ttatggcgtg aacggtcgaa ttagccaata tctgacgaaa atcggttgaa
    14581 aaagtggcat aatggggagt tgtcaactat tgaaatgaaa agtaaaacaa ttctcaacat
    14641 caaaccgtcg taacggatta cgcgatacga tataacatct ggaactttat tattacaact
    14701 caggccgtat gagcactttt aaaccactaa aaacactcac ttcgcgccgc caggtgctga
    14761 aagccggttt ggctgccctg acgttgccag gaatgtcgca agccatcgcc aaagacgaac
    14821 ctttaaaaac cagcaacgga cacagcaagc cgaaagccaa aaaatctggc ggcaaacgtg
    14881 tcgttgttct cgatccaggc cacggcggaa ttgataccgg agcgatcgga cgcaacggtt
    14941 cgaaagaaaa acatgtggtg ctggcgattg ctaaaaacgt ccgttccatt ttgcgtaatc
    15001 atgggattga tgcgcgttta acgcgttctg gcgatacgtt tatcccactt tacgatcgcg
    15061 ttgaaatcgc ccataaacat ggcgcagatc tgtttatgtc aattcatgcc gatggcttta
    15121 ccaacccgaa agctgccggt gcttcggtat ttgccctctc taaccgtggg gcaagtagcg
    15181 caatggcgaa atacctgtct gaacgcgaaa accgcgccga tgaagttgcc ggtaaaaagg
    15241 cgactgacaa ggatcaccta ttgcagcaag tgctgtttga tctggtgcaa accgatacta
    15301 ttaaaaacag cctgacgctt ggctcgcata ttctgaaaaa gattaagccg gtgcataaac
    15361 tgcacagccg caacaccgaa caagccgcgt ttgtggtgtt gaaatcgccg tcggttcctt
    15421 cggtgctggt ggaaacctcg tttatcacca acccggaaga agagcggctg ttaggcacgg
    15481 cggcgtttcg tcagaaaatc gccacagcga ttgctgaagg cgtgatcagt tatttccact
    15541 ggttcgacaa ccagaaagca cattcgaaaa agcgataagt tatgaaaccc gacgcacacc
    15601 aggttaaaca gtttctgctc aaccttcagg atacgatttg tcagcagctg accgccgtcg
    15661 atggcgcaga atttgtcgaa gatagttggc agcgcgaagc tggcggcggc gggcgtagtc
    15721 gggtgttgcg taatggtggt gttttcgaac aggcaggcgt caacttttct cacgtccacg
    15781 gcgaggcgat gcctgcttcc gccaccgctc atcgcccgga acttgccggg cgcagtttcg
    15841 aggcgatggg cgtttcactg gtagtgcatc cgcataaccc gtatgttccc accagccacg
    15901 cgaatgtgcg tttttttatt gccgaaaaac cgggtgcaga gcccgtctgg tggtttggcg
    15961 gcggcttcga tttaacccct ttctatggtt ttgaagaaga cgccattcac tggcaccgca
    16021 ccgcccgtga cctgtgcctg ccatttggtg aagacgttta tccccgttac aaaaagtggt
    16081 gcgacgatta cttctacctc aaacatcgca acgaacagcg cggtattggc gggctgttct
    16141 ttgatgatct gaacacgcca gatttcgacc actgttttgc ctttatgcag gcggtaggca
    16201 aaggctacac cgacgcttat ttaccaattg tagagcgacg taaagcgatg gcctacggcg
    16261 agcgcgagcg caattttcag ctctaccgtc gcggtcgtta tgtcgagttc aatctggtct
    16321 gggatcgcgg cactctgttt ggcctgcaaa ctggcgggcg caccgagtcg atcctgatgt
    16381 caatgccgcc actggtacgc tgggagtatg attatcagcc aaaagatggc agcccagaag
    16441 cggcgttaag tgagtttatt aaggtcaggg attgggtgta actccctcac ccccattccc
    16501 gcatccgctg atgcaacgtc aacgacggct tctcggcaaa cagctgctgg taatccgtgg
    16561 caaattgccc cagatgccag aatccccact gcatggcggc gtcttttacc gttgtgcttt
    16621 gcgaccacgg gcttatcagt tcgcggcgta cggcgttcag gcgaatgcgt ttcagccacg
    16681 cgtttgggcc aatgcctaaa atagcgtgaa acgcgttttg tagcgtgcgg cgactgacat
    16741 gcagttgatt acacaagtcc agcaccgtca ccagctcaga catattttcc agcacatatt
    16801 cacgggcgcg ggaaagtaat ctacggtaac tctgatgact gatgctttcc gccgtcacca
    16861 ttggttgcgc gtcttccagc atcgccccca tcgccattag caaattatcc cccagcactt
    16921 ttcgcactgc aggctgatgg agattttccg ggttctcgca aaacgtcgcc agcgcctgtt
    16981 ggacaaagcc ccacagcgcg gctttatgct gctctttcac ttccagcgcc gactggctac
    17041 gcagcatatg taatacccga tccggattgt gcaaaaagtt agcctgacgg gtgatgacat
    17101 cttcagacag cactacgccc aggatcgtat aatcgtccgg cgtacttaat tcaaactcgg
    17161 ttccaccggg gcgcgtcgct atttctgcgc ttcccagaca ttgcgaaccg ataaatccct
    17221 gctcaccgcg cgtcgccgga atgccaaacc agaacgagtt cggccagacc aggcacgact
    17281 gacgcagcgc cagaccggtg tattcacgaa aaacctgaat atcatcgagc agaatttccg
    17341 taaattcacc atgaaacttg cccggatgca gctgatcgta aatctgctgc caggcggtaa
    17401 tcgtcaaagc atgttcatag acatccgttg tctgtcgttg atgaacatta tccacttcga
    17461 ccttcggcgt gagcttcagg ttttcgggta agggttcatg ataaagatgg tgcaaattgg
    17521 ctgtacgggt ctttttcatg atgttaatgc cgggtgttgt aggacacccg gcacctccga
    17581 caggttaatg gggcttgaga cgataacgac tactgcgttt acgtaacgtc ccggcagaaa
    17641 agagctgttc gagcgcattt ctggcttttt ccagcggcca gccaaagtgg gcagccactt
    17701 ctcctgccgt cattccctga cgtactgatg ttaacagcgc caacaactcg tcagcagatt
    17761 ccgcgacgat aactggcgcg tcgggttcct ttacgggttg cttcggctgg cggttaaagc
    17821 cagtaaccag ccactgggtg tcatcgtctg gccgaccgat ctccttgcgg ctgatgacac
    17881 gcccggtacg catcgcggca gcacaacctg cgtccagcgc cgcacgacac gccgccagat
    17941 cgccttccac caccagcgtt aagcgaccag ggtcaagcac ttcgtgactg agcagacgaa
    18001 cgttagctgc tttgagcatg gcatccgctg catctatcgc ggcgaccatg ccgtccactt
    18061 ccagcaatcc cagtgcattg atcatcggca acctccgtta cgcacgctgg attggattac
    18121 gcgcgatttc cagcactgca tcggtaaagg cgttacaggc tgctttacac gcggcctggc
    18181 taccggttaa aaatgctgcc gagtagttgg tttcagacgg tggcgggaca taggtcacca
    18241 gctgtacgtc ggcagatttc aacgctgcat caatgccgta ggtcgcttcc agcggcggtg
    18301 ccaccagata cgccatcgga tcgccaagcg tgatcccggc ggttgatgaa agataagaac
    18361 cggtacgcga aactacatgt gccaggaatg cggtgttttc tgcgtcatta gcccactgga
    18421 aagcagcgcc attttcaata tgcgcaacca tcgcatccag accagcacgc acttccgccg
    18481 ggtttggccc accaagcata atcagcactt caccggcagt cggtgacggt ccgtgtgctg
    18541 ctcccgcata cagcgagcgg ccatatacca cttcgaccat cgcctgcttg gtcgcttcgt
    18601 cagccgcaat gtaggtgacg tcatcggaat cagcagaaat cagcccgagg ctacgaatat
    18661 gcggcggcaa ttttagttca cgcgcaaatt cagcgttaac ggaggcaatc acccgcatgg
    18721 cggttaccga cggtcgaatc aaatctaaag ctggcatgat gcctccttat cgggtcatgt
    18781 tgatgccgga tgctttctgc tccagcatac gtttcgccaa atccacaatg acggctgcgg
    18841 cttcaaccgg cggcgtgccg ccctggtgaa tgttagagat gcaggtgcga tcggcctcaa
    18901 cggtggtcgc catacgcggc gagtaaacgg cgtaacagga gaggctttct gactgaccca
    18961 accccggacg ttcccccacc agcaggatca ccactttcgc gccgaggatc tcaccgatct
    19021 gatcttcaat cttcacgcga ccataacgaa caaagaatgg cgtgccgact ttcagcccgg
    19081 cctgtttcag gcccgccatc agcggcggca ggatctcttc gtagttcacg gtgatggcat
    19141 cagttgacag gccatcagaa ataacgacct gtacgtccgg attagcaaca cactgtgctt
    19201 tcagcgcttc aacagcttct gcacacaggc ggcggcccat atccgggcga gtcaggtaga
    19261 gatttttgtc gctaatttcg gagcgtactt ccagcaagcc ctgcgctttc acccactctt
    19321 ccggcacttc tttcagtacg gtatctttcg aacgagagtg atcggccagg aaacgcagca
    19381 gcgcctgcgt acgtggacgc ggaccggtac gaccggtaca aacgcgggcc acggtgctgc
    19441 ggcgcagttc tgttaatacg tctgcgcgat gcgggttttc aacaccaatc cacgcttttg
    19501 cttccgcaga acctaaatcc agcgcgcagc tttccgaggt caccggtgcc gcacagttag
    19561 tggtggcgca ctttgcttct gacggcgcgg gggccgcttg tcccattgac gccatcacgc
    19621 tgcgtacaat ttcttcaatc tgtttttgat ccatgatgtg ttatctccgc gtcatcagaa
    19681 gaacagtgac ggatcgcccg cccgtttggt caggcgaccg tttgccataa tgcccatgct
    19741 ttccagccag cgttcaaact ccggtgacgg gcgcaggttg agtaactgac gcacagtggc
    19801 agtgtcgtgg aatgcggtgg tctgatagtt gagcatgatg tcatcgccca gcggcatccc
    19861 catgatgtag ttgcagcctg cggtggcgag caggatcatc aggttttcgt tgaggttctg
    19921 gtcagcgtca gcgtggttgg tgtaacagca gtcacagccc atagagatgc cgctcagctt
    19981 gcccataaag tgatcttcta agccagcacg gataatctgg cggtcgttgt agagatactc
    20041 cggcccaata aagccgacca cggtgttgac gataaacgga tcgtaatgac gcgccagccc
    20101 atagttacgt gcttccatcg tcacctggtc tgcgccgaag ttagcgccag cagacagcgc
    20161 cgaaccttgc ccggtttcga agtagaggca gttttccccg gcgatacggt tgaactcagc
    20221 acccactgcg cgcgcttcgt cgagcatcgc cagttccacg ccaaactctt tcagcccttt
    20281 ttcgctgcca cagatactct ggaaaatcag tccgccaggc gcgccgcgac gaatcgcttc
    20341 gatctgggtg gtgacgtgcg ccagtacgca gccctgagtt gggatgttga atttgtcgat
    20401 cacgccgtaa atggtgtcca acacgcggct taagttttcc acgtcgtcag ttaccgggtt
    20461 aacgccgatc accgcatcgc ccaccccgaa ggaaagccct tcgtagattt gtgcagcgat
    20521 actttgcacg tcgtcacggg tgtcatttgg ctgcaaacgg gcgctgaagg tgcccggaat
    20581 accgatggtg gtattggcct ttttgattac cggcattttc ttcgcgccgt agatcaggtc
    20641 cgcgttggag caaatcttcg ctaccgccgc gaccacttcc gaggtcagcc ctttgcgggt
    20701 aaaggcaatg tcgtccacgc tggtttcatc gctcagcaca tactcacgca gttcgctgat
    20761 gctcctgttt ttaatctggt tgtaggccgt ttcgttaaca tcgtcctgaa tcagccgcgt
    20821 cacgcagtca tcttcatagg caatcaccgg attattgcgg atgtccgcta cggtcatttc
    20881 cgacaacacc tgctttgccg ccacgcgctc ctgtgagctt gccgctgcaa cgcccgccag
    20941 cacatccccc gaacgcagtt cgttggcttt agccagcacc tcttttacat ccttaaactg
    21001 atatacattg ccgaacaatg tggtctttag tttcataagt cgttccctca ggaaggaaat
    21061 gcgagtgatt tcaccgtcac cggcacaacc gatccgccaa aaagaggcgt accaatgtcg
    21121 atatagtccc ccgcgcggac aatcacttcg tcaatgactg ccaacgggag ttgttgtagc
    21181 tgtgggcgca acaacatgcc cagagcttta ccaaagtcct gcccggccac caccagcagg
    21241 ggatgcggat ttggaaaacg cgcgacgaaa tcgaccagcg cgttgatgac cgtcagtacc
    21301 gcggcgtaac gcacaggcag cgaggcggga agcgccagca cgtacgcgtc agttttggga
    21361 tcaagatcca gctgaagcag cgcctgttgc caggcactca ccagatccgt ttcatcaatc
    21421 gggatcgcca ccggcaaatt gcgcagcggc agttgtacgc cctcaagcca gattgtgctg
    21481 ccagagagcg aaagggtatg cgcgcccgcg ccaatcaccg tggcgcgcac ggtttgcgcc
    21541 ggaaactgta cgttcatctc acgcaggcgc ggatggtcat gcagcgccgt tgccagcagc
    21601 gggccaatat cggcaaaaca gaacgggtcg gcgggctggt ggcgataaca ttcgcccacg
    21661 ccgccagaaa gcgtaatgat ttcgggcgta acacctgcgg gcagcaaacc ggtttgcatc
    21721 aatgcctgcg cgagcggcga gagcgttccg tcaatcactt cgacaatcag ttctgccatc
    21781 cgccgggtaa cctgcaccag ctgcgcgccg gtcagcgaac gggcgtcggt gcctgcgccg
    21841 aagcactcat ccacaatcat ctgccccggt ttatgagcgt aaaccacgcg cccctggctg
    21901 tcggtttcca gcaggcgacc accgacgttg aggcaggcag tgccgctgat ttttccggca
    21961 tcgaacaggg cgtagttcgc agtgccaccg ccgatgtcaa tattcagtac ccgacacagc
    22021 cgttgttcag aaagggtttg tgccccggca ccgtgaccgg caatgacgga ttcgaggtgc
    22081 ggcccggcgc tggcaacgac aaaatcgccc agcgactggg agagcaccat caccgccgga
    22141 cgagcattgc gggttttcgc gctttcaccg gtgatgatga tggcaccaga atcaacgctt
    22201 tccggcgcaa tgcccgcagc ctgatattgc tcgaggatta aggttttcag ttccgcttct
    22261 tttaaaccac cctgtttatc gacaggggta aagaacaccg ggctttgcca gctaatttcg
    22321 cgtttaatga attcgtagcg cggcacctgc gacaccgccg cacggttaac cagctccagg
    22381 tgggagaaaa tcacctgggt ggtggtggtg ccgatatcga taccgacgct caatagctgg
    22441 cgagtgttca cgattgtgcc tccgcttcgg ttttagccgt ggtcgcgtct tcttttggca
    22501 ccagcatcat cgccacgcca atcgccgtta cgccgccgat caacttgccg acaatcatcg
    22561 ggaagatcat ggcgttcatg ttggcagcgg cgaagcctaa gtggtcgccc agggcgaaag
    22621 cagcggaaac ggcgaaggcg cagttgatga ctttgccgcg ggtatccatc tgcttcatca
    22681 tgccgaacat cgggatgttg ttggcaagcg ttgccaccat gccggctgcc gcgatgttgt
    22741 tcatattcag cactttaccg acgctcatca gcggtttttc aaaccagcgg gtcagcagca
    22801 gcaccattgg atacgcccct aacagaacgc aggagataga accgataact tcaatggcgc
    22861 gcatcacttc accggtttta tcgccagggg ccataaagat agggtccaga ccggggatca
    22921 gttcccagcc aagcagaaac ttaactaccg cggcagcaag accgagggtg atcaatgcaa
    22981 cgaggaattt ggcgaagatc tggaagccgt tgatcatttt ttccgggatg aatttcagcc
    23041 ccagcgccac cagaatcgca acaatgatca ccgggatcat gttcatcagg atcagggcga
    23101 aagtgaattc caccggctga ccgttgatct gcacaccgga gtacatagca accagaccac
    23161 cagcaataca gccaatcgga atggtcacaa tgcccgccag cacgccgagc gccagataac
    23221 gacggtcaga aggttcgata atgccgagcg ccaccggaat ggaaaacaca atcgttggcc
    23281 ccatcatcga cccaagaatt aacccagagt atagccacgc tgctacgtcg ccgcccgcca
    23341 gctctttggc gaggaagaag ccgcccatat cgcacgccag cagcgttccg gcgaacattg
    23401 acgggtttgc gccgagcatt tcgtaaaccg ggataattac cggcccgagt acgtgagcca
    23461 gtaccggtgc cagcgcggtc ataccgacca tcgccagacc cagagcgccc attgccataa
    23521 agccttcttc gaactgaccg cctgatcctt cgatactttt accgaactta ccgaggaaac
    23581 gagcagaacc gccgaactgc gacaggatcc tgtctacggc agctatcagc ataaagaaca
    23641 tcatgatgta catgatgatt tcgttaattc ccatcgcctt tactccctgt tagttgttat
    23701 ttattggcgg atgcggcgta aacgccttat ccgccctaca tgtgcaatcc cgaaggccgg
    23761 atcagacgcg gcaagcgtcg catccggcaa ttgcaccgcg ccactggcgg atgcggcgtg
    23821 aacgccttat ccgccctaca tgtgcaatcc cgtaggccgg ataagacgcg gcaagcatcg
    23881 catccggcaa ttgcaccgcg ccactggcgg atgcggcgtg aacgccttat ccgccctaca
    23941 tgtgcaatcc cgtaggctgg ataagacgcg gcaagcgtcg catccggcat ttattgcgcc
    24001 gccgcgtaca gtccgacaat ctgttccagg ctggcggtac gcgggttact gcgcagacaa
    24061 atatcttcca gcgcggcctg cgcccatgcg ccgtaatgcg cagatgtcgc accaacatcg
    24121 cccagtcgtt taccaatccc aacttccgca atcagctcac ttaccgcgtt aatagcgtca
    24181 cgatcgtcgg attttttagt tcgcagtgcc cgaccaatct gactaaagcg ttcacgacaa
    24241 accatccggt taaattccat caccgttggc agcaacatgg cgttcgccag accgtgtgga
    24301 atatgcagcg ccgcgcccgg ctgatgcgcc atcgcgtggc acaacccaag acccgcactg
    24361 gaaaacgcca ttcccgccat acatgaagcc agcaacatgc tctcgcgcgc ggcaaggtcg
    24421 tgaccgtagc ccaccgcttt cggcagcgat ttgccaatca tcgcaatggc accaatcgcc
    24481 agactgtcgg taaacggtgt agcgttcagg gcgctgtatg cttcaatggc atgggttaac
    24541 gcatcaatgc cggtcatcgc cgtgacatgc gacggcacac cttcggtcaa cgcggcgtcg
    24601 agaatcgcca catccggcat cagcgaggca tgggctaaca cctgcttgcg cccgctcacc
    24661 gcgtcgataa tcaccgttac attggtggtt tcagagccgg ttccggcggt cgttggaatg
    24721 gcaatcagcg gcaagcgcgg ttgcagaacg ctggtttctg acatctctgc cagcgtgcta
    24781 tccgggttcg tcaccagcaa cgccacggct ttcgccgcat ccagcaccga gccgccgcca
    24841 aatgcgatca ccccatcaca gcctgactca cgcaactgcg ccacggctgc acacacgtcg
    24901 gtgatgcacg gttcgcccac cggacatggc cagagcgtca tggcgatgcc tttaacagcc
    24961 aggctgcgcg tcagcccggc ggtcatccct gcctgatgca aaaagctgtc tgccatcacg
    25021 aacagatgtt tcagcccacg cgtttgcgct tgctgtccgc aactgctcac cgagcccgga
    25081 ccgcaaagcg tcaccggtgg aacgctaaat gtttttaccc gttgcagatt cagggtatcg
    25141 aacgcctgaa agagcgcggt ctgcaattca ttttgcatat agcccctccg ctttctctcg
    25201 cccactactg gcgatcgcca gcggggtcat aaacaaactg tgctgcggta aatgcacctg
    25261 taacgccggg aattgtttgc gaaacagttc cgccacgccc ggttgcatac aagagccgcc
    25321 cgccagccat aaatcagtaa tgccctgccc ttcaatatgc cgggcgacaa tgtccgccat
    25381 tttttcgtac accggtttca ccgtaggcca aatctcttca ccgtgaccgc gcttgtactg
    25441 ctccgcctct tccagcgaaa tacggcgatt tccggcgaga gtcagagaga tgtgatgtcc
    25501 gccggttgct tcatccgccg agtacgtcac cttgcccttt ttcacgatgg caatgccggt
    25561 agtgccgccg ccgatatcca ctacaccggc gttgtccagt tgcagcagat ccgcaactgc
    25621 ggttggctca tccagcacat ggctcacttc cagtccagca gactccagca cgttgatgga
    25681 aatacgcggg tccgtacctg gaggaaatga agtcgccgca tggctaaaac ggcgaccgaa
    25741 ttgctgctcg agcgtgtcga gatggcgacg aacaatggtg acagcgccga agaaatccca
    25801 gacgatgccg tcgcggacca catcggccca gtcgaggcat accgccaccg actgaccgtc
    25861 gcggtcgaca accatcgaca ccacatcgca ggtgcccaga tccaccccca gccacagcgg
    25921 agattccgtc gcggcgggcg tctggttaca cagcgttgcc gccgtttgca gacgtggggt
    25981 gagccattgt tcgtcgtgcg ccatctgtta ctccttaaac aatgcgaaac gcatcgacta
    26041 atacacagcg acgcagacgg acaaacgtac gcacgctagt taccccttca ccggttggcg
    26101 tggtgatggt catggtggtc cagccttccc cgcccagccc cagcccggca atgcgcggtc
    26161 cgttcttaac gaaaatgctg gtatcaatag cattcgccat ctggttcatg ttttcgatgt
    26221 tgcgcgagtg cattgccgcc gtgtggtggc aaccgccttc cagtttcacc gccagcgcaa
    26281 tggcatccgc cacgttggcg acgcgcacga cgggcagcac cggcatcatc agttcggtca
    26341 cggcaaacgg atgttctgca gtggtttcca caaacagcaa acgcgtttct tgcggcacgt
    26401 tcaggccgat tgccgccgcg attttcgccg catcgcgacc aacccagtca cggctgacgg
    26461 tgccttttcc gcgctcgtcg atatttttca gcaacaccgg ttgcagctgc tgcgcctgtt
    26521 ctgcggtcag tttcaccgcg tgctggcctt ccatcagacg catcagttca tcggctacgc
    26581 tatcaacaac aatcagtacc ttttcgtcgg cacaaatgat gttgttatcg aaagaagcgc
    26641 ctttgacgat ggactgagcg gcacgggcga ggtcggcggt ttcatccacc actaccggcg
    26701 ggttgccagc gcctgcggca atcagacgtt tattggtgtg tttacgcgcc gcttctacta
    26761 ccgcttcgcc gccggtcacc accagaaggc cgatgcccgg aaacttgaac aaacgctgcg
    26821 cggtttcgat atccggattt gccacagtaa ccagtaagtt ttccggcccg cctgcggcaa
    26881 caatcgcctg gttgagcagc gtgatcgccc gctgggagac ttttttcgcc gccggatgcg
    26941 gggcaaaaat gacgctgttg cccgcggcaa tcaggctgat ggcgttatta attacggttg
    27001 ccgccgggtt agtggacgga gttaccgaag ccaccacgcc ccagggcgcg ttttcaatta
    27061 aagtcaggcc gttgtcgccg gtcagcacct gcggagagag acactcaacg cctggcgtgc
    27121 cgcgcgcctg agcgacgttt ttggcaaatt tatcttcaac gcggcccatg ccggtttcac
    27181 tgacggcaag ttccgctaaa tctctggcgt gtttttcgcc tgcctcacga atggcagcaa
    27241 tggctaactg gcgcattgcc acgcttttta acccttgctg ggcgactttg gctgccgcaa
    27301 cggcgtcatc cagggacgcg aaaacgccca tctcatgaac ggcggcgggc ggcgtgtcac
    27361 tgctttgcat tttcagcagt accgctttca ccacctgttc aatatcctgt tgattcatga
    27421 tgttctgcct tatttgtgga aaattacctg accgccagac accacctcat cgacaatgcc
    27481 aatcacgcac agatcgaccg gtgacgtttc gcttttatgc gcctggcggg cggaactgcc
    27541 actcaccagt aacacccact ccccggttcc cgcgccaata ttgtcgatgg cgacggcgca
    27601 ttgcccgtca ggattacctt gtggatcaat catttccacc atcagcaatt tgtcatgcgc
    27661 cagtccgtga tggcgtacgg tacaaacaat ttgtccagtg acgactgcca gtttcatacc
    27721 cgcctccgtg gcgtatttca ggtaaaagct ccccctaccc tccgcagaag gtaaaatgaa
    27781 aaaggagaga gcgtgacgcc cgaatcgacg tcacacaggg tgattacagg ttgctgctat
    27841 cgcctttcag gccgatcggg aagacttctt ccaggtcgcc gtgcggacgc gggataacgt
    27901 gtacagatac cagctcgccg atacgctgtg ccgctgcggc acctgcatcg gtcgcggctt
    27961 tacaggctgc aacatcacca cgaaccatgg cagtacacag gccgccgcca atctgcttaa
    28021 caccaaccag cttgacgcgc gcagctttaa ccatcgcgtc agaagcctca atcagtgcaa
    28081 ccaggccccg ggtttcgatc attcctaatg cttccattgt gctttcctct ttatcagggt
    28141 ccagaacggg accgttcatt caaccagtgt ttgtaaactg ctttcgcggt tcacttctgt
    28201 ctgacgcggc acagctgcca ccagcgccag ctcgataatt tcctgcacgc tacaaccacg
    28261 agagagatcg tgcatcggcg cggcaagtcc ttgtatcaat ggcccgacgg cacgatatcc
    28321 gccgagtcgt tgtgcgattt tgtagccaat atttccggct tccagcgacg gaaaaaccat
    28381 cacattggct ttgccctgta gcgggctggc aggcgctttt tgcgccgcca cttccggcac
    28441 gaaggcggcg tcaaactgca actcgccatc aaccaccagc tttggtgcgc gctcacggac
    28501 gatttctgtc gcctgctgga cgttggcaac gcaggggtga cgggcgctac cgttactgga
    28561 aaacgacagc atcgccacgc gcggctcttc tccggtgagg gcgcgccagg tttcggcact
    28621 ggcaagcgcg atatccgcca gttgcgccgc cgtcggctgt ggcaccacgc tgcaatcggc
    28681 aaagcccaac gccggaccgc tgtactgtgg cagcatcagg aaaatggatg agagcgtttt
    28741 acagcctggc tgcaagccga taatacgtaa tccggcacgc agcacattcg ccgtggaaga
    28801 gaggttgccc gcgatacaga catccgcttt accggcgctg accattgctg cggcgaacat
    28861 cagcggatcg gtaagttttt ccagcgcatc cggcggcgtt ttttcgcccg cgcgggccag
    28921 ccagcgatga gcaaattctt cccgcattgc gaggttgcca tgcggatcaa tcacctgtag
    28981 cccgtccatc gccacgccgt gactgagcgc aaactgacga agttcaaacg gattagcgac
    29041 cagaatgggc gttgccagac cttgttgatg taaatattgc gcagctttca gcacacgttg
    29101 atctaacgca tccggaaaaa ccactctggc gggcgctcgc aacgccagtt cacgacaacg
    29161 ttcaataatc attgcgtttc ccccagtcgt tgttggatct gcttcaccgt tagcggcggc
    29221 ttcgttacgc tcaggatcat cattacgtag accgtgctcg aaaggcggtt cagcgcctgc
    29281 aagatgtccg ggcgtaatac ttcaaagctg cgggtgataa acacctgcgc cgctacggtt
    29341 tcggtttccc gcactttggt acgtagcaga ttgagcaacg ccgcatcgcg cccgtggctg
    29401 gcttccggca ccagatgatc gtggtcgaga tagcgcagcg gctggtgaga aagtcgatgc
    29461 agatcttcgt cagaaagccc gacaatcgcc tggcagccca gcggttcccc cagcgcatcg
    29521 gcgcgcataa tgttgccaag acgcgaacgg atatccgcca gccacggctg ccacggttcc
    29581 gccagttcaa tttgcagcca cactgccagc gcaatggtgc tgtcgagaac cgcgcgaaaa
    29641 cccagacgcg gatcgctttt ggcgaccatt ttttccgccg acaggtgagt cagcgtatcc
    29701 ggcttttttg ccaccggctg gcggcacagt tcgcagcacg cctgcggatg ttcatcgcta
    29761 ctggtcagcc catgaacggg ctgcggctgc tgctgttcat cgtcaacaaa caggcggccc
    29821 tgctcgtcaa taaacttgat gcgcagatgg cggctttcca gcaactcccg ggcagagggc
    29881 gtcaggcgac tgtccgcagg gagatggatc tctgcgcctt cgctgagcgt atggttcgct
    29941 cttagccatg cttcggtgat gaaatctttc atagggattg ccagttagcc ggccaggcga
    30001 catacaggaa tttcacgctg gatgtcgtac caaattcgat gctggagcct ttcgggataa
    30061 acatcacgtc gcctgctttg gcgatcatgg tttcgccttc gtggcgaaca tggagctccc
    30121 cttccagcac catgtcgatt tcgtcgtagt tcagggtcca cgggaagaat gcgttctccc
    30181 actgcataaa accggcggcc atgctgctgc cgtcgtctcc ggtcacaaga tcagtcaagc
    30241 ccacgcaatg cggttgtgcg ccatcaaaac gaccaaactt gacgctgctg ccatcaatca
    30301 ctttgatgcc gcctttgccg gtcaccgatt tgaagctcgg ctgcattgcg ccctgctcca
    30361 gcgactgttt ttccttcatc actttttcca tcagctgtgc gaccaggctt tcggtaaact
    30421 ggccttccgg cagctgggca atgatggttt cgcggatgcg ctggctttcg gttttgtcgg
    30481 ctggcacgct ggcgggcacg gacgccgtta ccgggatcga ttcatcacat tcggtgatgg
    30541 taaaacccag tagatccgcc actttacggg cttccggggt aataatgctg gcgcgcagaa
    30601 ccacggacat tgcctgttcg ccgcgcgcgt gtgcttcacg aatatcattc gctgtgatga
    30661 gttttttcac ctgcttcctc ctgtttggtg agagatgcca gataatccac cagttgctgc
    30721 acgctttgcg ggtcgtggct gtttagctcg aaaatcggct cttcgaaacc cgtctcaagc
    30781 aataactttc gcgttgcggc gacgtcagca tctggcatgt ccgttttact gatgacggcg
    30841 atttgccgtt tgctgacgcc aatatccagc aacccggcag gcaaccgact ttccgggtca
    30901 tttgcgccat ggacatagat cagcatgtct acatcctgta gcgtggtaat taaggcgtga
    30961 taccagcggg gatggctaaa atattcaccc ggcgtatcaa tatcgccctt atcattaaat
    31021 tccacggcct gtgtttttct ggcgagggta taattcccct gcaacgcatt aaataacgtt
    31081 gtttttcctg ccccgacgct acccacaaac gcaattcgtt tcatgaatgc ctcgtattaa
    31141 cttttggtca tttcacaaag cgtataattt aataagcgac ctaaaccgct gacggtttgt
    31201 gataacgctt cttctactgc gcccaccgag ccataaatca ccaatgcacc gctgaagcgg
    31261 tcgagaaaac cgatatgcac atcagcggct ttcagcgcta aatcaccgac gatcatcgcc
    31321 gtttcgccgg gagttagcgt cataatgcca attgcgcccg catccggaac gccgattttc
    31381 ttcgccagtt cctcaccggg atgcgcgatg agatgcgcca gtgtgacctg tttgccgggc
    31441 acaaattcct gaatgatgcg ttctttatcc atgagtcgcc gcctgtgaaa aactttcgtg
    31501 tatcgtgaca atttttcgtc agagtaaata acaaaattcg gcaagcgcgt ttaaaaggtg
    31561 aggtagatca ctaagaatgg agagatattc gctgtggtgc ataaactttc atatgcaacg
    31621 catgaatata aataaaataa tatcgagata ttatatagac tgcttttttt tattattcgc
    31681 aaactcagta ataagcttta ctaatatatt gcagccaaac cgcttaccct gaatattcag
    31741 ggtaagcgtg agagttaaaa aagaattaca gcggttgggt ttgcgcttca accacggcca
    31801 gcgccaccat gttgacgata cgacgcaccg atgcgatcgg cgttaacacg tgaaccggtt
    31861 tcgccacgcc catcagcacc ggaccgacag tcacaccttc cgagctggaa acgcgcagta
    31921 agttgtaact aatgcgggca gcttccatgt tcggcatcac cagaatattg gcagaacctt
    31981 tcaaagggct gtccggcata cggtcgttgc gaatcgcttc caccagcgcc gcatcgccgt
    32041 gcatttcacc atcaatcatc agttctggcg cacgttccct gaccagttcc agcgcctgac
    32101 gcattttgct cgacgacggg cagtcagaag aaccaaagtt ggagtgcgac aacagagcaa
    32161 cgcgcggttc aataccgaaa cgacggacag tttctgccgc catcaaggtg atctccgcca
    32221 gctcttctgc gtctggttcg tcattgacgt aggtatcggc aataaaggtg ttaccactcg
    32281 gcagcagcag cgcgttcatt gcacctgcgg tgtgaacgcc atcgcgataa ccaaagacat
    32341 ttttcaccac gctaaaatgt tcatgataat cacccaccgt accgcaaatc attgcatcgg
    32401 cttcgccacg ctgaaccatg atcgcgccga tcactgtcgg gttactgatc agcgcccgct
    32461 gcgcctgttc ctgagtgacg ccgcgacgct tcatgatctg gaagtattcg gtccagtact
    32521 ctttaaagcg cggatcggat tcgttattga cgatctcaaa atcaacgccc gctttgatct
    32581 gcaagcccag tttctgaatg cgcatttcga tcacgttcgg acgaccgata aggatcggtt
    32641 tcgccagtcc cagcgttacc agttcctgag tggcatgcag aacgcgcgcc tcttcccctt
    32701 ccggcagaac tacgcgcttc ggcgctttgc gagcctggga gaaaatcggc ttcataaaca
    32761 ggttggtttt gtaaacgaac tcagtcagct tgtcgatgta gacgtcgaaa tcagcaatcg
    32821 gacgcgtcgc cacgcccgac tccatcgccg ctttcgcgac cgcaggagcg atcttaacga
    32881 tcaagcgcgg atcaaacggt tttggaatga tgtattccgg accaaagctc agatcctgat
    32941 cgccatacgc tgaagccacc acttcgctct gttccgcatg ggccagttct gcaatcgcgc
    33001 gtaccgccgc cagtttcatc tcttcgttga tggcggttgc gccaacgtcc agcgcgccac
    33061 ggaagatgaa cgggaagcaa aggacgttgt tgacctggtt tggatagtca gaacgaccgg
    33121 tgcagatgat ggcatccgga cgcacttctt tcgccagcgg cggcagaatt tccggttccg
    33181 gattcgccag cgccagaatc attggtgccc gagccatttt cttcaccatt tcctgggtca
    33241 gcactttcgg gccggaacag cccaggaaaa tatccgcacc ttcaatcaca tcatcgaggg
    33301 tacgtttgcc gtcatccacc accgcatatg cggctttggt ttccgccatg tttggctcac
    33361 ggccttgata gataacgcct tttgaatcgc aaaccacgat gttatgtttt tgcagaccca
    33421 gcgctaccag cagattcata caggcgattg ctgcggcacc cgcgccggaa accaccatcc
    33481 gcacgtcgga gatgtttttc tccaccacgc gcaagccgtt gaggatggcg gcagtgctga
    33541 tgattgccgt gccgtgctga tcgtcgtgga ataccggaat attcatccgc tcgcgcagtt
    33601 tctgttcaat atagaaacat tctggcgctt taatgtcttc gaggttgatt ccgccgaagg
    33661 tcggttcgag cgcagcgaca acttcaataa atttgtccgg gtcgagttca tcaacttcaa
    33721 tgtcaaatac atcaatcccg gcgaatttct taaacagaac gcccttgcct tccatcaccg
    33781 gtttgccagc cagcgcgcca atgttgccta accccagcac cgccgtaccg ttagagatca
    33841 ccgccaccag gttaccgcgg gcggtatatt tgtaggcttt taacgggtct ttttcgattt
    33901 caagacaagg tgcggcaacg ccgggtgagt aggccagcgc cagatcgcgc tgcgttgcca
    33961 gaggcttggt tggagaaacc tggattttcc ctggaactgg aaattcatgg aaatcaagtg
    34021 cactttgttt taactggtca tccatttggt tgttcctttc acgtaacgtt cacaaataaa
    34081 gtgtgtgggc aacaccccct gcccacaacg tggcgcacat tattaccttg ccggagtcta
    34141 cagactttga gcaagtccaa actctcacca ttaatataat gttttggtaa taatcctata
    34201 acactgatgt tacctgctta atccagcaat accatgcctg tctgctatgc ttttttgatg
    34261 cgtttagcga aatttctcag aagtgtgaat taacgcactc atctaacact ttacttttca
    34321 aggagtattt cctatgaacg agttagacgg catcaaacag ttcaccactg tcgtggcaga
    34381 cagcggcgat attgagtcca ttcgccatta tcatccccag gatgccacca ccaatccttc
    34441 gctgttactc aaggctgccg gattatcaca atatgagcat ttaatagacg atgctatcgc
    34501 ctggggtaaa aaaaatggca agacccagga acaacaggtg gtcgcagcgt gtgacaaact
    34561 ggcggtcaat ttcggtgctg aaatcctcaa aatcgtaccc ggtcgcgtgt caacagaagt
    34621 tgatgcacgc ctctcttttg ataaagaaaa gagtattgag aaggcgcgcc atctggtgga
    34681 cttgtatcag caacaaggcg ttgagaaatc acgcattctg atcaagctgg cttcgacctg
    34741 ggaaggaatt cgcgcggcag aagagctgga aaaagaaggt attaactgca acctgacgct
    34801 gctgttttct tttgcacagg cacgggcctg tgcggaagca ggcgtttttc tgatttcgcc
    34861 gtttgtcggg cgtatttatg actggtatca ggcacgcaag ccgatggacc cgtatgtggt
    34921 ggaagaagat ccgggcgtta aatcggtgcg caatatctac gactactata agcaacacca
    34981 ctatgaaacc attgtgatgg gcgcgagctt ccgtcgcacc gaacaaatcc tcgccttaac
    35041 cggctgcgat cgactgacta tcgcaccgaa tttgctgaag gagctgcagg aaaaagtttc
    35101 gccagtggta cgtaaattaa tcccaccttc tcagacgttc ccacgcccag ctcccatgag
    35161 cgaagcggag ttccgttggg agcacaatca gtatgcgatg gcggtagaaa aactgtctga
    35221 aggcattcgt ctgttcgccg ttgatcaacg caaactggaa gatcttcttg ccgccaaact
    35281 ataaaccagc cacggagtgt tatatgtccc gaaaagacct tgccaatgcg attcgcgcac
    35341 tcagtatgga tgcggtacaa aaagccaatt ctggtcatcc cggcgcgccg atgggcatgg
    35401 ctgatattgc cgaagtgctg tggaacgatt ttcttaaaca taaccctacc gacccaacct
    35461 ggtatgatcg cgaccgcttt attctttcca acggtcacgc gtcgatgctg ctctacagtt
    35521 tgctgcatct gaccggttac gacctgccgc tggaagagct gaagaacttt cgtcaactgc
    35581 attcgaaaac cccaggccac ccggagattg gttatacgcc aggcgttgaa accaccaccg
    35641 gtccacttgg acaaggtttg gcgaacgccg tcgggctggc gatagcagag cgtacactgg
    35701 cggcgcagtt taaccagcca gaccatgaga tcgtcgatca cttcacctat gtgtttatgg
    35761 gcgacggctg cctgatggaa ggtatttccc acgaagtctg ttcgctggca ggcacgctgg
    35821 gactgggcaa gctgattggt ttttacgatc acaacggtat ttccatcgac ggtgaaacag
    35881 aaggctggtt taccgacgat acggcaaaac gttttgaagc ctatcactgg catgtgatcc
    35941 atgaaatcga cggtcacgat ccgcaggcgg tgaaggaagc gatccttgaa gcgcaaagcg
    36001 tgaaagataa gccgtcgctg attatctgtc gtacggtgat tggctttgga tcgccgaata
    36061 aagcaggtaa ggaagaggcg cacggcgcac cgctggggga agaagaagtg gcgctggcac
    36121 ggcaaaaact gggctggcac catccgccat ttgagatccc taaagatatt tatcacgcct
    36181 gggatgcccg cgaaaaaggc gaaaaagcgc agcagaggtg gaatgagaag tttgccgcct
    36241 ataaaaaggc tcatccgcaa ctggcagaag agtttacccg tcggatgagc ggtggtttac
    36301 cgaaggactg ggagaaaacg actcagaagt atatcaatga gttgcaggcg aatccggcga
    36361 aaatcgccac ccgtaaggct tcgcaaaata cgcttaacgc ttatggcccc ttgctaccgg
    36421 agctgctcgg cggttcggcg gatctggctc ccagcaacct gaccatctgg aaaggttctg
    36481 tttcgctgaa ggaagatccg gcgggcaact acattcacta cggggtgcgt gaatttggca
    36541 tgaccgctat cgccaacggc atcgcgcacc acggcggctt tgtgccgtat accgcgacgt
    36601 tcctgatgtt tgttgaatac gcccgtaacg ccgcgcggat ggcggcactg atgaaagcgc
    36661 ggcagattat ggtttatacc cacgactcaa ttggcctggg cgaagatggt ccgacgcacc
    36721 aggctgttga gcaactggcc agcctgcgct taacgccaaa tttcagcacc tggcgaccgt
    36781 gcgatcaggt ggaagcggcg gtgggctgga agctggcggt tgagcgccac aacggaccga
    36841 cggcactgat cctctcaagg cagaatctgg cccaggtgga acgtacgccg gatcaggtta
    36901 aagagattac tcgtggcggt tatgtgctga aagacagcgg cggtaagcca gatattattc
    36961 tgattgccac cggttcagag atggaaatca ccctgcaagc tgcggagaaa ttagcaggag
    37021 aaggtcgcaa tgttcgcgtg gtttccctgc cctcgaccga tattttcgac gcccaggatg
    37081 aggaatatcg ggagtcggtg ttgccttcta acgttgcggc tcgcgtggcg gtggaagcag
    37141 gtattgccga ttactggtac aagtatgttg gtctgaaagg ggcaattgtc gggatgacgg
    37201 gttatgggga atctgctccg gcggataagc tgttcccgtt ctttggcttt accgccgaga
    37261 atattgtggc aaaagcgcat aaggtgctgg gagtgaaagg tgcctgatag tgatcgccgg
    37321 atgctgattg ccggatgcga cgctgacgcg tcttatccgg cctacagcgt cttatccggc
    37381 ctacatgtcc ccgccatttt gtttaacggg tgatccacaa cgtgggccag gcgtctggcc
    37441 catgccagtt atcgcaggtg ggttctgcgg cgtaacgcac caggcgaaaa cgctgaccgt
    37501 caaagcgcca gcgcgcctga atgccacaat cgcttaatcc gcgccctttc gctaaggtca
    37561 ccagttcacg cgatttctca tcaaatgttg cgttcatcag ttccagttca ttcgtctcct
    37621 gcccgttgtt gaacggcaaa cgcaaccgaa ccgggcgcga ggctagcggc tttttacgcg
    37681 acacaatcca tgccaaatca atggtgttat aggcccctgc ctcacagctg atcatcatca
    37741 gcgctttatc atcagtcagc gcagtgacat tcacctcacg acgtaacgga tcaagcgagc
    37801 agcgcagacc attcatccgc cagtttccat aatccagcaa atcgttgcgt tcttcgagtg
    37861 agagtggcgt cggcgttggg ttaaccaccg cgacctcttt cagcgcaggc gcgggcggta
    37921 cgctgagcgg cggttcgtcc cctttcttga tccacgcggt ttcactgcca acgcgctttt
    37981 gctgagcatc aataaacaac aacgccgctt ttaagccact cagagaaatg gtctgattgc
    38041 cgtcgcgtaa ggtgatcgcc ttcccttcct gaatcatctg caaaaacgcg gtgatggttg
    38101 ccgtatcatc ggttactaat aaccatggtg aaatccgcca cttgtcacca cttaacgcca
    38161 acggctcacc atctaacagc agccgtggcg ctatctctcc ttcagacgca tccggcgact
    38221 tcaacccgcc gcgctcaata cgtaaaacgg catcggtatg cgccccggcg ctgcggctca
    38281 gggtcatcac cagtccatta tgatcgcccg tattgcgcgc cacgcagaag ttttggttat
    38341 tacaggtgac ctgccagtcg gaaaacgccc gttgcgctag tgccgcccac accaaagacg
    38401 tcggcaacaa agcgaaaaaa aagagaagga aaatgcgata gcgcatggac ggtacgaccc
    38461 cagaagttca aacaaacagc acaagtcgta tcttcgttgc gcaggtagcg cggctcaatc
    38521 agatttgtcg gattgagcca ataataaaca acgaaatatt attgttattt ttcagtccat
    38581 taaatgtgac gtttgcaaat agttaagcaa taacaccgtc ttaccgtcac gtatctcgcc
    38641 ggttttgatc atctccagcg cctggctgaa cggcagctcg agcacttcaa tatcttcatc
    38701 ttcaacaccg ccaccggcgt tagcgcgctg attgtcactg tattcggcga taaaaaagtg
    38761 gattagctca gtcacaccgc caggcgacat atacagttca aataatttgc gcacttcgcc
    38821 cacttcatag cccgtctctt caatcgcttc tttacgaatg cacacttccg gttcgtcgtt
    38881 atccagcagc ccggcgcagg tttcaatcag ctgcccgctt tcattgccat taacccaggt
    38941 agcgacacgg aactgacgaa tcagaaccac ggtctttttc ttcgcgttgt acaggaggat
    39001 cgtcgcacca ttaccgcgat cgtaaacttc acgtttatgg cggataactt agccatcttt
    39061 gcgcgtgaga tcgtaagtaa tgttgtgcag ggtgaaatag ttatcggaga gaattttgtc
    39121 tttaatgagg gtgatttgtt gcgtcatacc gactccacag cgcgaaatga acaattatat
    39181 tacgctgtga agtcgggttt gtctgcgcag gctatcagtg agatttgact gattttaccc
    39241 ccagccagtc aataatcccc tgtgccgcat gacgtccttc tgccattgcg gtaaccacca
    39301 gatccgcacc acgcacggcg tcgccaccgg cgaagatttt cggattggtg gtctggtaac
    39361 ggtactggct ttccacatcc gcgatgatgc ggccccattt gtctaccgtt acaccgtgcg
    39421 actccagcca cggcatcccg tgtggattga agccaaacgc cataatcacc gcatcggctg
    39481 gcatgacaaa ttcactgccc tcaatcggta caagtcgccg gcgcccctgg gcatccggct
    39541 cgccaagacg cgtgcgcagg aaacgaattc cgcaaacgtg accttgctca ttcagctcaa
    39601 gcgccaccgg ctggacgtta aattcgaagt tggccccctc ttcgcgggcg ttcttcacct
    39661 ctttcttcga gcctggcatg ttagcttcat cacgacgata agcgcaggtg acgttactcg
    39721 cgccgtggcg cagcgcggta cgcacacagt ccatcgcggt gtcgccgccg cccagtacca
    39781 cgacgttgag tccggcggta ttgataaacg gctcttccgg tagctcttcg agtcccatca
    39841 cctgtttagt gttggcgatg aggaacggca gcgcgtcata aacgcccggc gcatcttcat
    39901 tgggtaaacc cgctttcatg gaacggtaag tgcctacgcc aacgaagacc gcgtcgtatt
    39961 gttccaaaag cgaatccaaa gagacatctt tacccacttc acaattgagt tcgaagtgaa
    40021 tccccatcgc gctgaagatt tcccgacggc gtgccagcag ggatttatcc agtttgaaag
    40081 aaggaatgcc gaaagtgagc aagccaccaa tttctggatg gcgatcgtac accgtcaccg
    40141 ccacgccatt gcgggtcaga acatccgcac aggccagccc tgccggaccc gcaccgataa
    40201 tcgccacccg cttgtccact ttggtgacat ggcttaagtc aggacgccaa cctttcgcca
    40261 acgcctgatc tgaaatgtag cgttcaatgt tgccgatagt taccgcgccg tgctcatcgc
    40321 gaatagtaca ggcaccttca cacaaacggt cctgcggaca aacgcgtccg gtaatttccg
    40381 gcagggtgtt ggtcaggtga gaaagctcga cggcggcgtc gatgtttccg gctttcacca
    40441 gttcaatcca ctgcggaata tggttatgca gcgggcaggt ccattcacaa acgctgtgct
    40501 cgccgcattt aaggcagcgc gaggcttccc gttgtgcctg atcggcacgg aatggcagat
    40561 aaatttcatc aaaaccggtt ttgcgcgctt caatcgccag tttatccggc tcgccacgcg
    40621 cgggcgttgc ctgcatttgt tcgactttac tcattaccgg catttcttgt gcagcggtac
    40681 tggcatgcca cggttgatgc tcctgacgtg cggtgcgcaa gcggcgggat ttcgccatgc
    40741 cggagagtgc gacgtcagtg accagttgca gcgcgtccgc cgggcaattc tcgacacagg
    40801 caggaccgtt ttcgcgccct gcacaaaggt cgcatttatg cgccgtggct tttacttttc
    40861 ctgccgcgac gggtgtcagg acgatttgca tcgtaccaaa cggacaggcc accacgcagg
    40921 atttacagcc aatacacttt tgctgattga cctgaatgct gtcatcaacg tggctgattg
    40981 cgccattagg gcagctacgg gcgcagggcg catcttcaca atggtgacag gtcactgcac
    41041 tacgttgctg ctgatgtttg ataaccgtaa ttcggggatg aaaatggtgt tggctcagga
    41101 catgttgctc atcattgtga gccatgacac aggcgatttc acaagcatga caacccagac
    41161 attgctgact gttggccata ataaaacgat tcataacgac cttctttttt ggttgtaaaa
    41221 accttattct ttatatgagt gttgttatta cccgacttac aggggaatcg gcaatgttca
    41281 tgtgcccaga ataagtaact atttcgctat aaactgtggc agatcaaata atcccatcac
    41341 tgactaaatt gcgttttagc gaactggaac attaatgatt ttttgtggag aagacgcgtg
    41401 tgattgttaa acgacccgtc tcggccagtc tggcccgggc ctttttttac attgtgctgc
    41461 tgtcgattct ttccacgggt atcgctctgc taactctggc gagcagtttg cgcgacgctg
    41521 aggctatcaa tattgccgga tcgctgcgta tgcagagtta ccgcctgggc tacgacttgc
    41581 aaagtggcag tccacaactc aatgcacatc gccagttgtt tcagcaggca ctgcattcat
    41641 cggtattaac caatctcaac gtctggtatg tgccagaagc agtaaaaact cgctatgcgc
    41701 atctgaatgc caactggctg gagatgaata atcggctcag caagggcgat ttgccgtggt
    41761 atcaggccaa tattaataat tatgttaatc agatagacct gttcgtgctg gctttacagc
    41821 actacgctga acgcaaaatg ctgctggtgg tggcgatttc cctggctggc ggtatcggta
    41881 ttttcacgct ggtctttttt actttgcgcc gtatacgcca tcaggtggtt gccccgctga
    41941 atcagctggt taccgccagt caacgtattg aacacgggca gttcgactcg ccgccgctgg
    42001 ataccagtct gccgaatgag cttggtctgc ttgcaaaaac ctttaaccag atgtcgagcg
    42061 agctgcataa attgtacctt tcgctggagg cgtcagtaga agaaaagacc cgcgatctcc
    42121 acgaggccaa gcgtcgtctg gaggtgttgt atcaatgttc gcaggcgctg aacacaagcc
    42181 agattgatgt gcattgtttc cgccatattt tgcagattgt tcgcgacaat gaagcggctg
    42241 aatatctgga gttaaatgtc ggtgaaaact ggcggattag cgaagggcaa ccaaacccgg
    42301 aattgccgat gcagatttta ccggtgacaa tgcaagagac ggtttacggc gaactgcact
    42361 ggcaaaatag tcacgtttca tcatcagaac cgctgcttaa cagcgtttcg tcgatgctgg
    42421 gacgcggttt gtactttaat caggcgcaaa agcattttca gcagttattg ttgatggaag
    42481 aacgtgcaac catcgcccgc gaattgcacg actcgctggc tcaggtactt tcttacttac
    42541 gtatccagtt gacgttactg aagcgttcga taccggaaga taacgccacc gcacaaagta
    42601 tcatggccga tttttcccag gcgttgaatg atgcttatcg gcagttacgc gagctgttga
    42661 ctactttccg cctgacgctg cagcaggcgg atctcccctc cgcgttgagg gaaatgctgg
    42721 atacgttaca aaatcaaacc agcgccaaac tgaccctcga ctgccgtctg ccaaccctgg
    42781 cgctggatgc gcaaatgcag gtgcatttgt tgcaaattat tcgcgaagcg gtgctgaatg
    42841 cgatgaagca cgccaacgcc agcgaaatcg ccgtcagttg cgtcaccgcg ccggacggca
    42901 atcacacggt ttatatccgt gataacggga ttggtatcgg tgaaccgaaa gaacccgaag
    42961 gtcattatgg tctgaatatc atgcgcgaac gcgcggaacg gctgggtggg acgctgactt
    43021 tttcacaacc ttccggcggc ggcacgttag tgagtattag ctttcgctct gcggagggtg
    43081 aggaaagtca gttgatgtaa tgcctcctac tgaccaaaga atacttgcac ttaaggttca
    43141 gtataaaagg gcatgataat ttacattaac tccttttttt ctccacgatt ggctcgtacc
    43201 ttgccgctac agtgaagcaa gtcaagccta caacgatacg cagaaacacg aggtcctctt
    43261 ttaatggcga atttctttat tgatcgcccc atttttgcct gggtgctggc aatcctgttg
    43321 tgtctgacag ggaccctggc gattttttca ttgcccgttg aacaataccc cgatctcgcg
    43381 ccaccgaatg tgcgagtgac cgctaactat cccggcgcat cggcccagac gctggaaaac
    43441 accgtgaccc aggttatcga gcaaaatatg accggcctcg ataatctcat gtatatgtca
    43501 tctcagagca gtggcatcgg tcaggcatct gtcactttaa gttttaaagc aggcaccgat
    43561 ccggacgaag ccgtgcagca agtacaaaac cagctgcaat cagccatgcg aaagttaccg
    43621 caggcggtgc aaaatcaggg cgtgacggtg cgtaaaaccg gcgataccaa tattctgacc
    43681 attgctttcg tctctaccga tggttcgatg gataaacagg atattgccga ttatgttgcc
    43741 agtaatattc aggacccgtt aagccgcgtg aatggcgtcg gggatatcga tgcctatggt
    43801 tcgcaatatt ccatgcgtat ctggctggac ccggcgaaac tcaacagttt ccagatgacg
    43861 gctaaagatg tcactgatgc cattgagtca cagaacgcgc agattgcggt tgggcaactt
    43921 ggtggtacac cttccgtcga taagcaggcg ctcaacgcca ccattaacgc ccagtcactg
    43981 ctgcaaacac cagaacagtt ccgcgatatc accttgcggg tgaatcaaga cggctcagag
    44041 gtaaggctgg gcgatgtcgc caccgtcgaa atgggggcgg agaaatacga ttatcttagc
    44101 cgcttcaatg gtaagccagc ctccgggctg ggggtaaaac tggcctccgg cgcaaacgaa
    44161 atggcgacag cggagctggt gctcaatcgt ctcgacgagc tggcgcagta cttcccgcac
    44221 ggactggaat acaaggtggc gtatgaaacc acctcgtttg ttaaagcctc cattgaagac
    44281 gtggtgaaaa cgctgctgga agctatcgcc ctggttttcc tcgttatgta tctgttcctg
    44341 caaaacttcc gcgccacgct gatacccact atcgccgtgc cggtggtgtt gatgggaacc
    44401 ttctccgtac tttacgcctt cggttacagc gtcaacacct taaccatgtt cgcgatggta
    44461 ctggcgatcg gtctgctggt ggatgacgca atcgtggtgg tggaaaacgt cgaacgtatt
    44521 atgagtgagg aaggactcac tcctcgcgaa gccacacgta aatcgatggg gcagatccag
    44581 ggggcactgg tcgggattgc gatggtgctt tcggcggtat ttgtaccaat ggccttcttc
    44641 ggcggcacca ccggtgccat ctatcgccag ttctctatta ccattgttgc ggcgatggtg
    44701 ctgtcagtac tggtagcgat gatcctcact ccggctctgt gtgccacact acttaagcca
    44761 ctgaaaaaag gtgagcatca cgggcaaaaa ggcttttttg cctggtttaa ccagatgttt
    44821 aaccgcaacg ccgaacgcta cgaaaaaggg gtggcgaaaa ttctccaccg cagcctgcgc
    44881 tggattgtga tttatgtcct gctgcttggc ggcatggtgt tcctgttcct gcgtttgccg
    44941 acgtcgttct taccgctgga agaccgtggc atgtttacta cctcggtaca attgcccagc
    45001 ggttcaaccc aacaacagac cctgaaagtc gttgagcaaa tcgagaaata ttacttcacc
    45061 catgaaaaag acaacatcat gtcggtgttt gccaccgttg gttctggccc tgggggtaac
    45121 gggcaaaacg tggcgcgaat gtttatccgc ctgaaagact ggagcgaacg cgacagtaag
    45181 accggcacct cgtttgccat tatcgagcgt gcaacaaagg cgtttaacaa gattaaagaa
    45241 gctcgcgtta tcgccagcag cccgccagca attagcggtc ttggtagttc tgcgggtttt
    45301 gatatggagt tgcaggacca cgctggagca ggtcacgatg cgctgatggc agcacgtaac
    45361 cagttgctgg cgctggcggc ggaaaacccg gagctaaccc gtgtgcgcca taacggcctc
    45421 gacgacagtc cgcagttgca gattgatatc gaccagcgta aagctcaggc gctgggcgtt
    45481 gctatcgacg atattaacga cacactgcaa accgcctggg gttcgagcta tgtgaatgac
    45541 tttatggatc gcggtcgcgt gaagaaagtc tatgtgcagg cagctgcgcc gtatcgcatg
    45601 ctgccagatg acatcaatct ctggtatgtc cgaaataaag atggcggcat ggtgcccttc
    45661 tctgctttcg cgacctcacg ctgggaaaca ggctcgccgc gtctggaacg ctataacggt
    45721 tattctgcgg ttgagattgt tggggaagcc gcaccggggg tcagtaccgg tacggcgatg
    45781 gatattatgg aatcgttagt gaagcagctg ccaaacggct ttggtctgga gtggacggcg
    45841 atgtcgtatc aggagcgact ttccggcgcg caggctccgg cgctgtacgc catttccttg
    45901 ctggtggtat tcctgtgtct ggctgcgttg tatgaaagct ggtcggtgcc gttctcggta
    45961 atgctggtcg tgccgctggg ggtaatcggc gcgctgctgg caacctggat gcgcgggctg
    46021 gaaaacgacg tttacttcca ggtgggcctg ttaacggtca ttggtttatc ggcgaaaaac
    46081 gccatcctga tcgtcgagtt tgctaacgag atgaaccaaa aaggccacga cctgtttgaa
    46141 gcgacgctcc acgcctgccg tcagcgttta cgcccgattc tgatgacctc gctggcattt
    46201 atcttcggcg tattgccaat ggcaaccagc acgggtgccg gttccggtgg tcagcatgcg
    46261 gtgggtactg gcgtaatggg cgggatgatt tcagccacta ttctggctat ttacttcgtg
    46321 ccgctgttct ttgtgctggt gcgccgccgc ttcccgctaa agccgcgccc ggaataagca
    46381 ataaaaaagg cgacatgcca atgtgtcgcc tttttcaact ttccgattaa gaacctgctc
    46441 agcgggttct tgctgtttat actttgtctc aggaattact tacgaagcat aacttcgata
    46501 aagtctttcc agttccccag ttcacgttca atcataacaa cctctcttat aattatgggt
    46561 attctacgga aacaatatac cgtggtgaag ctaatttact cgattgctgc gatgactacc
    46621 tccgggggga caaatcttat gtaaatacta tggtcctgcg gtgatgattt gtatgtgata
    46681 cacagcaaca tttcgagata ttcatacggc atctaatact gatttaattc aggtta
//
LOCUS       DAZPLI010000015        46701 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZPLI010000015 DAZPLI010000000
VERSION     DAZPLI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.015"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHUU3_000798"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHUU3_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR2080958.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="ACHUU3_000799"
     CDS             complement(280..873)
                     /locus_tag="ACHUU3_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR2080959.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="ACHUU3_000800"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="ACHUU3_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR2080960.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="ACHUU3_000801"
     CDS             1788..2183
                     /locus_tag="ACHUU3_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR2080961.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHUU3_000802"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHUU3_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR2080962.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHUU3_000803"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHUU3_000803"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR2080963.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHUU3_000804"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHUU3_000804"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR2080964.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="ACHUU3_000805"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="ACHUU3_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR2080965.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHUU3_000806"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHUU3_000806"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR2080966.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHUU3_000807"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHUU3_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR2080967.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="ACHUU3_000808"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="ACHUU3_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR2080968.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="ACHUU3_000809"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="ACHUU3_000809"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR2080969.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="ACHUU3_000810"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="ACHUU3_000810"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR2080970.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHUU3_000811"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHUU3_000811"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR2080971.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHUU3_000812"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHUU3_000812"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR2080972.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHUU3_000813"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHUU3_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFR2080973.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHUU3_000814"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHUU3_000814"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR2080974.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHUU3_000815"
     CDS             17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHUU3_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR2080975.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHUU3_000816"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHUU3_000816"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR2080976.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="ACHUU3_000817"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="ACHUU3_000817"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR2080977.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHUU3_000818"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHUU3_000818"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFR2080978.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHUU3_000819"
     CDS             complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHUU3_000819"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR2080979.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHUU3_000820"
     CDS             complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHUU3_000820"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR2080980.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHUU3_000821"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHUU3_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFR2080981.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="ACHUU3_000822"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="ACHUU3_000822"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR2080982.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="ACHUU3_000823"
     CDS             complement(26207..26548)
                     /locus_tag="ACHUU3_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR2080983.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHUU3_000824"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHUU3_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR2080984.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHUU3_000825"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHUU3_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR2080985.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="ACHUU3_000826"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="ACHUU3_000826"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR2080986.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="ACHUU3_000827"
     CDS             33227..34570
                     /locus_tag="ACHUU3_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR2080987.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34667..34873)
                     /locus_tag="ACHUU3_000828"
     CDS             complement(34667..34873)
                     /locus_tag="ACHUU3_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR2080988.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35198..35752
                     /locus_tag="ACHUU3_000829"
     CDS             35198..35752
                     /locus_tag="ACHUU3_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR2080989.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35842..36753)
                     /gene="ytfI"
                     /locus_tag="ACHUU3_000830"
     CDS             complement(35842..36753)
                     /gene="ytfI"
                     /locus_tag="ACHUU3_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFR2080990.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPV"
     gene            complement(36965..37705)
                     /gene="cysQ"
                     /locus_tag="ACHUU3_000831"
     CDS             complement(36965..37705)
                     /gene="cysQ"
                     /locus_tag="ACHUU3_000831"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR2080991.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37895..39838
                     /gene="cpdB"
                     /locus_tag="ACHUU3_000832"
     CDS             37895..39838
                     /gene="cpdB"
                     /locus_tag="ACHUU3_000832"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR2080992.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSNKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(39956..40336)
                     /locus_tag="ACHUU3_000833"
     CDS             complement(39956..40336)
                     /locus_tag="ACHUU3_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR2080993.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40425..41285
                     /gene="qorB"
                     /locus_tag="ACHUU3_000834"
     CDS             40425..41285
                     /gene="qorB"
                     /locus_tag="ACHUU3_000834"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR2080994.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            41393..42358
                     /locus_tag="ACHUU3_000835"
     CDS             41393..42358
                     /locus_tag="ACHUU3_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR2080995.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            42466..43128
                     /gene="ytfE"
                     /locus_tag="ACHUU3_000836"
     CDS             42466..43128
                     /gene="ytfE"
                     /locus_tag="ACHUU3_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR2080996.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43173..44585)
                     /gene="cycA"
                     /locus_tag="ACHUU3_000837"
                     /gene_synonym="dagA"
     CDS             complement(43173..44585)
                     /gene="cycA"
                     /locus_tag="ACHUU3_000837"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR2080997.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(44894..45514)
                     /gene="fklB"
                     /locus_tag="ACHUU3_000838"
     CDS             complement(44894..45514)
                     /gene="fklB"
                     /locus_tag="ACHUU3_000838"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR2080998.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            45733..46371
                     /gene="ytfB"
                     /locus_tag="ACHUU3_000839"
     CDS             45733..46371
                     /gene="ytfB"
                     /locus_tag="ACHUU3_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFR2080999.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(46355..>46701)
                     /locus_tag="ACHUU3_000840"
     CDS             complement(46355..>46701)
                     /locus_tag="ACHUU3_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR2081000.1"
                     /translation="PWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKK
                     HNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILS
                     WLLVDPSSTAHE"
BASE COUNT        11460 a        12420 c        11633 g        11188 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtatcgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgctg
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagagc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gaccccagaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cgactttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ttactgcata gcacttttta ctgcatagca ctttggttaa gccatctctg
    34681 tttgcagacg cataacctga cggttaactt cggacatcac tgacaaatgc agcttatcct
    34741 tcacttttgg gataagaatc ttacccttat caaattcaaa agcgccaacg tccttgatgt
    34801 ataaccgtcc acggaacagg atctttacgt acttcgccac ctgaagtggg ttgtaccgtt
    34861 ggaaaatttt cattgttatc tcctgctgag tattacgcct gtgcggtgcc acaatcggcc
    34921 caactattat gaggcgcaaa ttttaatgct tagtgactat agactatccg ggcaatgttt
    34981 ccaccgcgta taactttttt tacctaaagg ttacaattat tcagaattat ctttttaccg
    35041 aagcgcggtc ttcagtataa gcattcattt ttcatatgat ttgtgcgctt gaccgcaaac
    35101 tggcatcaca cttgcgggaa attcgataaa tagcacatat gattaaaact cagacccaag
    35161 tggtcggatc acctgcatat cataagaagg aaacaccatg accctacgca agattctggc
    35221 actcacctgc ctgctgttgc cgatgatggc ttccgcacat cagttcgaaa ccggtcagcg
    35281 agtgccgccg attggcatta ccgatcgggg cgagttggtg cttgataaag atcagtttag
    35341 ctacaaaacc tggaacagcg cgcagttagt gggaaaagtg cgagtactgc aacatattgc
    35401 tggtcgcacc tctgcaaaag agaaaaacgc gacgctgatt gaagcgatta aatcagcgaa
    35461 gttaccgcac gatcgttacc agaccaccac tattgttaac accgacgacg cgattccggg
    35521 ttcagggatg tttgtgcgca gcagtctgga gagtaataaa aagctttatc cctggtcgca
    35581 gtttattgtc gatagcaatg gcgtcgcgcg cggtgcctgg cagctggatg aagaaagttc
    35641 cgctgtagtg gtgctggata aggatggtcg cgtgcaatgg gccaaagacg gggcgctcac
    35701 tcaggaagag gtgcagcaag tgatggacct gctgcaaaag ttgctcaaat aatggcaaca
    35761 acatagattc tgcaaaaata attttcccct atcgttatct atgggatagg ggaattaagt
    35821 ctctgagttc ttacgccgaa ctcatactgg taacgctagt tgctcttttt taaacgcctc
    35881 aatggtcgac ttatatttct cgcaaatctt ttctttggta atattactat ctgcggccag
    35941 attcgcactt actatcgcat ccacttgttc ggctaattcc acttgttggc aacctgcatt
    36001 aaccagaaaa ttataaataa gggagtaatc ttcatcctcc aaaacaacag aggaaccatt
    36061 acgaaaacgt tcatcccctc catagatttt aagcgcaaag cccaaagatt ctatgactat
    36121 ggcaaaatta tcatcaatta ttttttcgtg aatataccat ccatcgcatt ctttaattcc
    36181 acctcctggt atttggagtt caaatttacc ccgttttaat ttgcttacta tatcctccaa
    36241 taaggcataa cctaaagaca cgtcaccaat cgcctggtga atataacctt taatcctttt
    36301 cggtatgtca gactgttttt tctgcagaat atcacaggca tacaatcttc tgttttgttc
    36361 taacggaaac tcgcggtttt tgaaatccat gaatgagtta tactcattta ttgttatggg
    36421 aatttcttcg tattcaaatc cactacctcg caatgtcatt gtatattcgg caccgttatc
    36481 tttaaggctg aaactgataa ggaacttatc catggcgtta acagttccat tttttgatga
    36541 aagtacctga taactatttt tatcaatctg agttgcgcaa cgaaagaact ccctcaaagt
    36601 taacaggcta tcataattcg aaatccaatc ttttttctta aatgcatgct ggatattgtt
    36661 aaaaaaattg tttgaatgtg tttttgtata cgatgacttt acaaataact ccaccgcacc
    36721 aataaaatta ttgtgtctga tcctgggtaa catatgacaa ccttatttta tttggttatt
    36781 ttatgtcgcc attaaatatc tcattagaga agtaaactat caaaaaacgc tcattattaa
    36841 aatgtaactt attagcaatt gaatttttgc actttaaata caatctcttt ttatttataa
    36901 ctttttaggt tgggagaaat caggatggca gaatcaggaa atacactgtt tctgccatct
    36961 gaatttagta aatagacact ctgaaccccg gattcaggaa cgactcgcgc ggagtgtaat
    37021 ccagcggttt accctgccag tcgtgaacgt gcgctccggc agctgcagcc acagcatgtc
    37081 cagcggcggt gtcccaaata ttcgttggtc cgaagcgcgg gtacagctgc gcctgtcctt
    37141 ccgccaccag gcagaatttc agcgaagagc cgatggacgt ggtctgatgt tcgccaagct
    37201 gttgcagata ctctttcagc tccgcatctg catgggaacg gctgataacc accagcggcg
    37261 ggcgcgcatc gcggacctga atctgcttgc gcacaccgca ctcttctttc caggccttgc
    37321 cttctgccgc gctgtacatt acgttcatta ccggcgcata caccacacct aaaatcggtt
    37381 tgccatggtc aatgagcgca atgttaacgg tgaattcgcc attacgttta ataaactctt
    37441 tagtaccatc cagcgggtct accagccagt aacgctgcca gtgctgacgg acttcccaac
    37501 cgggaggatc ttcttcagaa aggaccggaa tatccggtgt cagcgtacgt aaaccgtcca
    37561 tgataacggt gtgtgcggca atatccgctg ccgttaccgg agaattgtcc gctttgctga
    37621 cgacgtccat cggtttcgtc ccgtcgtaga cctgcataat ggcatcgcct gcattccgtg
    37681 caagctggca tacttgatct aacatttctc cacctcgtct ctgtgagcgg tgttaactta
    37741 ttgttttact tataccctat cgttaatgaa tgcgccaact gtgatagtgt catcattttc
    37801 aaagcgtaaa attgtggcat tcttcactgt tctataagta agacgtttat tctccctttt
    37861 ctttcgtatt cccgatgata aaaggatgtc cctgatgatt aagtttagcg caacgctcct
    37921 ggccacgctg attgccgcca gtgtgaatgc agcgacggtc gatctgcgta tcatggaaac
    37981 cactgatctg catagcaaca tgatggattt cgattattac aaagacaccg ccacggaaaa
    38041 attcggactg gtacgtacgg caagcctgat taacgatgcc cgcaatgaag tgaaaaacag
    38101 cgtactggtc gataacggcg atttgattca ggggagtccg ctggcagatt acatgtcggc
    38161 gaaaggatta aaaacaggtg atgttcatcc ggtttataag gcgctgaata cgctggatta
    38221 tacggtcggt acactcggca atcatgaatt taactacggt ctggattacc tgaaaaatgc
    38281 gctggcggga gcgaaattcc cttatgtaaa tgccaacgtc attgacgcca gaaccaaaca
    38341 gccaatgttt acaccgtatt taattaaaga taccgaagtg gtcgataaag acggaaaaaa
    38401 acagacgctg aagattggct atattggcgt cgtgccgccg caaatcatgg gctgggataa
    38461 agctaattta tccgggaaag tgacggtgaa tgatattacc gaaaccgtgc gcaaatacgt
    38521 gcctgaaatg cgcgagaaag gtgccgatgt cgttgtcgtt ctggcacatt ccgggctgtc
    38581 tgccgatccg tataaagtga tggcggaaaa ctcagtttat tacctcagtg aaattccggg
    38641 cgttaacgcc attatgtttg gtcatgctca cgccgttttc ccgggtaaag attttgctga
    38701 tatcgaaggg gctgatatca ccaaaggcac gctgaatggt gttccggcgg taatgccggg
    38761 catgtggggc gatcatcttg gggtggtcga cttacaactc agtaatgaca gcggtaaatg
    38821 gcaggtgacg caggcgaaag cggaagctcg gccgatttac gacatcgcca ataaaaaatc
    38881 cctcgcggcg gaagacagca agctggtaga aacactcaaa gccgatcacg atgccacacg
    38941 ccagttcgtc agcaagccaa tcggtaaatc tgccgacaat atgtatagct atctggcgct
    39001 ggtgcaggac gatccgaccg tgcaagtcgt gaacaacgcg caaaaagcgt atgtcgaaca
    39061 ttacattcag ggcgatccgg atctggcaaa actgccggtg ctttcagctg ccgcaccgtt
    39121 taaagttggt ggtcgcaaaa atgacccggc aagctatgtg gaggtggaaa aaggccagtt
    39181 gaccttccgt aatgccgccg atctttatct ctaccccaat acgctgattg tagtgaaagc
    39241 cagcggtaaa gaggtgaaag agtggctgga gtgctccgcc ggacagttta accagattga
    39301 tcctaacagc acgaaaccac agtcactcat caactgggat ggtttccgca cttataactt
    39361 tgatgttatt gatggtgtga attatcagat tgatgttacc cagcccgccc gttatgacgg
    39421 cgagtgccag atgattaata ccaatgcgga aaggattaag aacctgacct ttaatggcaa
    39481 gccgattgat ccgaacgcca tgttcctcgt tgccaccaat aactatcgcg cttacggcgg
    39541 caaatttgcc ggtacgggcg acagccatat cgcttttgct tcaccggatg agaaccgctc
    39601 ggtgctggca gcgtggattg ctgatgagtc gaaacgtgcg ggggaaattc acccggcggc
    39661 agataacaac tggcgtttag caccgatagc tgccgataag aaactggata tccgtttcga
    39721 aacttccccg tcaaataaag ccgcagcgtt tattaaagag aaagggcagt atccgatgaa
    39781 taaagtcgcg accgatgata tcgggtttgc gatttatcag gtggatttga gtaagtaaaa
    39841 cacttctttt tcggcctata aatcatcaat cgcatccggc atttattggc ggatgcgatg
    39901 ctggcgcatc ttatccgccc tacaagtcat acaccgtagg ccgcgcatcc ggcagttatg
    39961 ccgcacgttc atcccgcacc gccaacacct cgggcaaatt caactcaatc cagtccgcca
    40021 gtgcagcaac cttttcgctc acctgctcgc ccagcggcgt gaggctgtat tcaacatgcg
    40081 gtggcaccac cggatacgcg atacggttaa gaaaaccatc ctgttctaac gcctgtaacg
    40141 actgcgcaag catcttttcg ctcaccccgc cgattttgcg ccgcaggtcg ctaaagcgat
    40201 gagtaccttc gcgtagcgcc accagaatca acacccccca acggctggtg acgtgtttca
    40261 acacctcgcg cgacgggcac tgttccgcaa agagattacc ctctttcagt tgttgcgaca
    40321 ggctaacctg actcatttca tacttacctt tttgtacgta cttactaaaa gtaagtttag
    40381 gtgttagcgt atttaaacac aagacaaaac gatggagact tcccatgatc gctattactg
    40441 gtgccactgg ccaacttggt cactatatta ttgaatcctt gatgaaaacg gttcctgcca
    40501 gccaaatagt ggctatcgtt cgtaatccgg caaaagccca ggctctagca gcacaaggca
    40561 ttaccgtgcg tcaggctgac tacggcgatg aagccgcact gacatctgca cttcagggag
    40621 tggaaaaact actgctgatc tcttccagcg aagtgggtca acgtgccccg cagcatcgta
    40681 atgttattaa tgccgcaaag gcggctggcg tgaaatttat cgcttatacc agcctgctac
    40741 atgcagatac ctccccgctc ggcctcgccg atgagcacat tgagacggag aaaatgctgg
    40801 ctgattctgg catcgtttac accctgctgc gcaacggctg gtacagcgaa aactacctcg
    40861 ccagcgcccc ggcagcactg gaacacggcg tatttatcgg tgcggcgggc gatggcaaaa
    40921 tcgcctcagc aacgcgggca gattatgcgg cagctgcggc acgcgtgatt agcgaagccg
    40981 gtcacgaagg caaggtttac gaactggcgg gcgatagtgc ctggacgttg acacagttag
    41041 cggcagagct gaccaaacag agcggcaaac aggttaccta tcaaaatctg agcgaagccg
    41101 atttcgccgc ggcactgaaa agcgtcggac tgcccgacgg actggcggat atgctggcgg
    41161 attctgacgt tggcgcatcg aaaggtggtc tgtttgataa cagcaaaacg cttagcaaat
    41221 tgattggccg cccaacgaca acgttagccg aaagcgtaag ccatcttttt aatgttaata
    41281 actagttaat taaagtggca tcctcccgca tcctctctga taatgacggg atgccgggag
    41341 caatcatgtc tgcttcctga actttcttct gacagaccaa tggatgccag taatgattag
    41401 cggcgtgctg tacgccctgt tagcagggtt gatgtggggg cttatttttg tcgggccgtt
    41461 gatcgtaccg gaatacccgg cgatgttgca gtcgatgggg cgttatctgg cgttagggtt
    41521 aattgcgctg cccattgcct ggctgggacg cgtgcgtctg cgtcagttgg cgcgtcgcga
    41581 ctggcttacc gccttgatgc tcaccatgat gggcaacctt atctattact tctgccttgc
    41641 cagtgccatt caacgtactg gcgcgcctgt ttccacgatg attatcggca ccctgccggt
    41701 ggtgattccc gtttttgcca atctgcttta tagccagcgc gacggcaaac tcgcatgggg
    41761 aaaactcgcc ccggcactgg tttgtattgg catcggcctg gcgtgtgtga atattgctga
    41821 gttaaaccac ggactccccg attttgactg ggcacgttat acctctggca tcgtgctggc
    41881 gttagtttcc gtggtctgct gggcgtggta tgccctgcgc aacgcccgct ggctgcggga
    41941 aaatcccgac aaacatccga tgatgtgggc aacggcgcag gcgctggtca cactgccggt
    42001 ttctctcatc ggctatctcg tcgcctgtta ctggctgaat atacaaacgc cggacttctc
    42061 cttacctttt ggcccccgtc cgctggtgtt tattagtctg atggttgcga tagccgtgct
    42121 ttgctcatgg gttggcgcac tctgctggaa cgtcgccagc cagcgattac cgacagtgat
    42181 tctcgggccg ctgattgttt tcgaaacgct ggcaggtttg ctgtacacct ttttactccg
    42241 ccagcaaatg ccgccgctaa tgacgctgag cggtatcgcg ctgttagtga ttggcgtggt
    42301 cattgcggtc agagcaaaac cagaaaaacc tttaactgaa tctgtctcag aaagttgaca
    42361 cgctggcagt gagttaaata agcctctgct acgtaagggt tatagctttt gccttaaaga
    42421 tgcatttaaa atacatctta tattattaag aatgaggtat cagctatggc ttatcgcgac
    42481 caacctttag gtgaactggc gctctctatt cctcgcgctt cagctctgtt tcgtaaatat
    42541 gatatggatt actgctgtgg cggtaagcaa acgctggcgc gcgcggcggc acgtaaagaa
    42601 ctggatgttg aggtcattga agctgaactg gcaaagcttg ctgaacaacc gattgagaaa
    42661 gactggcgta gcgccccgct ggcagaaatc attgaccata tcatcgtgcg ctaccacgat
    42721 cgtcaccgcg agcaactgcc ggagctgatc ctgcaagcga ctaaagtcga gcgcgttcac
    42781 gccgacaaac cgagcgtgcc aaaagggctg acaaaatacc tgactatgct gcatgaagag
    42841 ctatccagcc acatgatgaa agaagagcag atcctcttcc cgatgatcaa acaaggcatg
    42901 ggcagccagg caatgggacc aatcagcgtg atggaaagcg agcacgatga agcgggcgaa
    42961 ctgctggaag tgattaaaca caccaccaat aacgtcacac cgccgccgga agcctgcacc
    43021 acctggaaag cgatgtataa cggtattaat gaactgattg atgacctgat ggatcacatc
    43081 agcctggaaa acaatgtact gttcccacgc gcgctggcgg gtgagtaatc taaaagctgg
    43141 atggcattgc gccatccagc atgataatgc ggttatttcc gcagttcagc agcccgcttc
    43201 ttaccaataa acaaccagcc cagccccagt gcgataaacc acagcggggt gaccaacagc
    43261 gcctggcgag tgtcatcttc cagtgtcagc aacacgacca cgaacacaaa gaacgccata
    43321 catacccagc acatcagctt gccgagcggc atcttgtaga tcgacttctc atgcagatga
    43381 ggacgctgtt tgcggtaaac caggtacgag caaaggataa ttgtccagac gaacataaac
    43441 agaatcgcgg aaacggttgt aatcatcgtg aacgcgccaa tcacgctagg attcacatac
    43501 aacatcacca cgccgccgag cagacagata cacgagaagg tcaacccttt cgcgggtact
    43561 gcgcgcttag aaagtttagc gaacgctttc ggtgccacac cttcctgcgc cagaccaaac
    43621 agcatacggc tggtagagaa gacgccgctg ttagcggaag acgccgcaga ggtcagcacc
    43681 acaaagttga tcacgctggc agccgcaggc aaacctacca acacaaacag ttcaacaaac
    43741 gggctcttct ccgggactac cgaactccac ggcgtcacgg acataatcac aatcagcgcg
    43801 aagacgtaga acataatgat acggatcgga atggagttaa tcgcgcgtgg cagtgatttc
    43861 tccggatctt tggtttccgc agcggttgta cctaccagct caatccctac gaaagcgaaa
    43921 accgctatct ggaatccggc aaagaagcca cttaaacctt tcgggaacca gccgccatca
    43981 ttccacaaat gcgcgaatga cgcttccaca ccagtcggtg actgaaagtg catcgccacc
    44041 ataaccaggc cgacgacaat cagcgacaca atggcgacga ttttgatcat cgcaaaccag
    44101 aactccatct caccgaacat tttcacggtg gcgagattga gcgtcagcag cagcactatc
    44161 accgccagcg aggcgaccca gtcggagaga tcggggaacc agaactgagc ataagcagtg
    44221 atcgccacca cgtctgccat accggttaca acccagcaga accagtaagt ccagccggtg
    44281 aaatatcctg cccacggccc gagtaaatcg gaagcgaagt cactaaaaga tttgtattcc
    44341 agattcgaaa gcagcaattc ccccattgcc cgcatcacga aaaagagcat aaaaccgatg
    44401 atcatataaa cgaaaatgat cgacggcccg gcaaggctaa tcgttttgcc ggaccccata
    44461 aacaaccccg taccaatggc accgccaatg gcaataagct gaatatgtcg gtttgtgaga
    44521 ttgcgccgta gcgactgttc agccggagcc tgatcatcgg caacgacttt tacctgatct
    44581 accatgtttt tttcttcctg tacctgtctg tgttgttcag gctctacggc ctttagtcag
    44641 tctatgatac gacgaatctg tatcgggact catcgatatt aggtaacaat acgcgggatg
    44701 aatactaaga tttagatcta atgttaattt tatgtttaaa gtgagtgtca tatcacggtg
    44761 ataacgcgaa acagctcaca aaaatacacg cttacggggt ctttgcaaca caaaatagcg
    44821 ccgtttggca aactgaaggg tttattgctg aatgcctgct cccctctggt tcgaggggaa
    44881 cagaatgcgc tgcttagagg atttccagca gttcgacttc aaacaccagg gtgctgaacg
    44941 gagggatgga tgcgcctgcg ccgcgctcgc catatgccag ttcctgcggg atagtcagtt
    45001 cccatttaga acctaccggc atcagagtca gtgcttcaat ccagccaggg atcacgccat
    45061 taaccgggaa ttcagcgggt tcaccacgag caacggagct gtcaaacacg gtgccgtcga
    45121 tcagtttacc ggtgtaatga acacgaacgc ggtcggtacg tgccggaatt gcgccttcac
    45181 cctggttgat cacgcggaat tgcaggccag attcggtgct attcacacct tcttttttgg
    45241 cgttttcttc caggtatttc acaccttcag cagccatcgc ctggaaacgc tgacgacgaa
    45301 cggcatcggc gcgttcgtgg atttcacgca gcgcgcgatg caccacatca accggcacag
    45361 ccggatgttt gccttccagc gcatcggcaa tacctgcaac cagtgcttct ggcagcagcc
    45421 cttccaggcc agattcactc agttgttgcc ctacctgcaa accaatgccg tagcttgctt
    45481 gcgcttcgat ggtgtcaaaa gttggggtgg tcatcgtatt tcctttctta gggtataaaa
    45541 gtagcgggca gcatatcagc cacacttctt tgggtaaaac tttgtcgcag gaatgatgac
    45601 aaatcccggg gaaaaagaaa caatagcaat atcccgtatg aatataataa aggcgggttt
    45661 accacggaca tcatagcgtt agcttattca taggctatga ttgaggaaca agacgcggag
    45721 caggaggaaa ccatgcccgg gcgctttgaa ctaaaaccaa ccctggagaa agtctggcac
    45781 gcgccggaca attttcgctt catggacccg ctgccgccga tgcatcgtcg ggggatcatt
    45841 attgccgcca ttgtattggt ggtcggattt ctgcttccat ctgatgatac gcccaacgcg
    45901 ccggtggtga cgcgtgaagc gcagttagat attcagtcgc aatcacaacc gccgacagaa
    45961 gaacagctgc gggcgcaatt agtcacacca caaaatgatc ctgatcaggt ggctccggtc
    46021 gctccagaac ctatacagga aggccagcca gaagaacagc cgcaaactac ccaaacgcaa
    46081 ccattccagc cagatagcgg aattgataat caatggcgct cttaccgtgt cgaacccggt
    46141 aaaacgatgg cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt ctatgcaatg
    46201 gcgcaggtag aaggcgcggg taagccgctg agtaatttgc aaaatgggca gatggtgaaa
    46261 atccgccaga acgccagcgg cgtggtgacg ggcttaacca ttgatacggg gaataatcaa
    46321 caggtcctgt ttacccgcca gccggatggc agttttattc gtgcgcggta gatgaagggt
    46381 caaccaaaag ccagctaagg attgtctctt tgatgacctt acgtctggct tccagtgcct
    46441 ggggtgaaac catactgatg tgataaacca ggccaagcgt atgctggttg atcaaataat
    46501 atcccccaag cgcagcaata ttaatattca cctgcaatgg gtcgatatct ggcttaaaaa
    46561 tattgtgttt cttcccttca tccaataagg attccattaa ctgcaagtgc gcatggttaa
    46621 tttctaagag acgcagtgat tttgcataat gcacaccttt actttgattt tcactatgca
    46681 caatttttaa aaaccaaggg t
//
LOCUS       DAZPLI010000016        46014 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZPLI010000016 DAZPLI010000000
VERSION     DAZPLI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46014
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.016"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..715)
                     /locus_tag="ACHUU3_000841"
     CDS             complement(<1..715)
                     /locus_tag="ACHUU3_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR2081001.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSS"
     gene            complement(729..1388)
                     /gene="pgmB"
                     /locus_tag="ACHUU3_000842"
     CDS             complement(729..1388)
                     /gene="pgmB"
                     /locus_tag="ACHUU3_000842"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR2081002.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAVCAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1385..3652)
                     /gene="ycjT"
                     /locus_tag="ACHUU3_000843"
     CDS             complement(1385..3652)
                     /gene="ycjT"
                     /locus_tag="ACHUU3_000843"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR2081003.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3649..4704)
                     /locus_tag="ACHUU3_000844"
     CDS             complement(3649..4704)
                     /locus_tag="ACHUU3_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR2081004.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4714..5502)
                     /locus_tag="ACHUU3_000845"
     CDS             complement(4714..5502)
                     /locus_tag="ACHUU3_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR2081005.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5520..6572)
                     /locus_tag="ACHUU3_000846"
     CDS             complement(5520..6572)
                     /locus_tag="ACHUU3_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR2081006.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6603..7445)
                     /locus_tag="ACHUU3_000847"
     CDS             complement(6603..7445)
                     /locus_tag="ACHUU3_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR2081007.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSAGNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(7432..8313)
                     /locus_tag="ACHUU3_000848"
     CDS             complement(7432..8313)
                     /locus_tag="ACHUU3_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR2081008.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8334..9644)
                     /locus_tag="ACHUU3_000849"
     CDS             complement(8334..9644)
                     /locus_tag="ACHUU3_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR2081009.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9679..11189)
                     /locus_tag="ACHUU3_000850"
                     /pseudo
     CDS             complement(9679..11189)
                     /locus_tag="ACHUU3_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            complement(11401..11715)
                     /gene="pspE"
                     /locus_tag="ACHUU3_000851"
     CDS             complement(11401..11715)
                     /gene="pspE"
                     /locus_tag="ACHUU3_000851"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR2081010.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11790..12011)
                     /gene="pspD"
                     /locus_tag="ACHUU3_000852"
     CDS             complement(11790..12011)
                     /gene="pspD"
                     /locus_tag="ACHUU3_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR2081011.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(12020..12379)
                     /gene="pspC"
                     /locus_tag="ACHUU3_000853"
     CDS             complement(12020..12379)
                     /gene="pspC"
                     /locus_tag="ACHUU3_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR2081012.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12379..12603)
                     /gene="pspB"
                     /locus_tag="ACHUU3_000854"
     CDS             complement(12379..12603)
                     /gene="pspB"
                     /locus_tag="ACHUU3_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR2081013.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12657..13325)
                     /gene="pspA"
                     /locus_tag="ACHUU3_000855"
     CDS             complement(12657..13325)
                     /gene="pspA"
                     /locus_tag="ACHUU3_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR2081014.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13492..14469
                     /gene="pspF"
                     /locus_tag="ACHUU3_000856"
     CDS             13492..14469
                     /gene="pspF"
                     /locus_tag="ACHUU3_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR2081015.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14587..15852)
                     /gene="puuE"
                     /locus_tag="ACHUU3_000857"
                     /pseudo
     CDS             complement(14587..15852)
                     /gene="puuE"
                     /locus_tag="ACHUU3_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15890..17170)
                     /gene="puuB"
                     /locus_tag="ACHUU3_000858"
     CDS             complement(15890..17170)
                     /gene="puuB"
                     /locus_tag="ACHUU3_000858"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR2081016.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(17172..18662)
                     /gene="puuC"
                     /locus_tag="ACHUU3_000859"
     CDS             complement(17172..18662)
                     /gene="puuC"
                     /locus_tag="ACHUU3_000859"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFR2081017.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDHAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEEAGLPDGVL
                     NVVSGYGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANI
                     VFADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGH
                     PLDTVTTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNA
                     SLSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSV
                     FVNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(18812..19369)
                     /gene="puuR"
                     /locus_tag="ACHUU3_000860"
     CDS             complement(18812..19369)
                     /gene="puuR"
                     /locus_tag="ACHUU3_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR2081018.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19396..20160)
                     /gene="puuD"
                     /locus_tag="ACHUU3_000861"
     CDS             complement(19396..20160)
                     /gene="puuD"
                     /locus_tag="ACHUU3_000861"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR2081019.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20372..21733
                     /gene="puuA"
                     /locus_tag="ACHUU3_000862"
     CDS             20372..21733
                     /gene="puuA"
                     /locus_tag="ACHUU3_000862"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR2081020.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21882..22046
                     /locus_tag="ACHUU3_000863"
     CDS             21882..22046
                     /locus_tag="ACHUU3_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2081021.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            22036..23421
                     /gene="puuP"
                     /locus_tag="ACHUU3_000864"
     CDS             22036..23421
                     /gene="puuP"
                     /locus_tag="ACHUU3_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR2081022.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23555..23800
                     /locus_tag="ACHUU3_000865"
     CDS             23555..23800
                     /locus_tag="ACHUU3_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR2081023.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            24113..25756
                     /gene="sapA"
                     /locus_tag="ACHUU3_000866"
     CDS             24113..25756
                     /gene="sapA"
                     /locus_tag="ACHUU3_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR2081024.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25753..26718
                     /gene="sapB"
                     /locus_tag="ACHUU3_000867"
     CDS             25753..26718
                     /gene="sapB"
                     /locus_tag="ACHUU3_000867"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR2081025.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26705..27595
                     /gene="sapC"
                     /locus_tag="ACHUU3_000868"
     CDS             26705..27595
                     /gene="sapC"
                     /locus_tag="ACHUU3_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR2081026.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27595..28587
                     /gene="sapD"
                     /locus_tag="ACHUU3_000869"
     CDS             27595..28587
                     /gene="sapD"
                     /locus_tag="ACHUU3_000869"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR2081027.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28589..29395
                     /gene="sapF"
                     /locus_tag="ACHUU3_000870"
     CDS             28589..29395
                     /gene="sapF"
                     /locus_tag="ACHUU3_000870"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR2081028.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29463..29816
                     /locus_tag="ACHUU3_000871"
     CDS             29463..29816
                     /locus_tag="ACHUU3_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR2081029.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            30184..30972
                     /gene="fabI"
                     /locus_tag="ACHUU3_000872"
     CDS             30184..30972
                     /gene="fabI"
                     /locus_tag="ACHUU3_000872"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR2081030.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            31117..32243
                     /locus_tag="ACHUU3_000873"
                     /pseudo
     CDS             31117..32243
                     /locus_tag="ACHUU3_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32311..34245
                     /gene="rnb"
                     /locus_tag="ACHUU3_000874"
     CDS             32311..34245
                     /gene="rnb"
                     /locus_tag="ACHUU3_000874"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR2081031.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34323..34426)
                     /locus_tag="ACHUU3_000875"
                     /pseudo
     CDS             complement(34323..34426)
                     /locus_tag="ACHUU3_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34480..36470
                     /gene="pdeR"
                     /locus_tag="ACHUU3_000876"
                     /pseudo
     CDS             34480..36470
                     /gene="pdeR"
                     /locus_tag="ACHUU3_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(36452..36580)
                     /gene="ymiD"
                     /locus_tag="ACHUU3_000877"
     CDS             complement(36452..36580)
                     /gene="ymiD"
                     /locus_tag="ACHUU3_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR2081032.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            36617..36790
                     /gene="yciZ"
                     /locus_tag="ACHUU3_000878"
     CDS             36617..36790
                     /gene="yciZ"
                     /locus_tag="ACHUU3_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR2081033.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36880..37629
                     /locus_tag="ACHUU3_000879"
     CDS             36880..37629
                     /locus_tag="ACHUU3_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR2081034.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37898..38116
                     /gene="osmB"
                     /locus_tag="ACHUU3_000880"
     CDS             37898..38116
                     /gene="osmB"
                     /locus_tag="ACHUU3_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR2081035.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38242..38568)
                     /gene="yciH"
                     /locus_tag="ACHUU3_000881"
     CDS             complement(38242..38568)
                     /gene="yciH"
                     /locus_tag="ACHUU3_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR2081036.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38568..39305)
                     /gene="pyrF"
                     /locus_tag="ACHUU3_000882"
     CDS             complement(38568..39305)
                     /gene="pyrF"
                     /locus_tag="ACHUU3_000882"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR2081037.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39498..40667)
                     /gene="lapB"
                     /locus_tag="ACHUU3_000883"
     CDS             complement(39498..40667)
                     /gene="lapB"
                     /locus_tag="ACHUU3_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR2081038.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40674..40982)
                     /gene="lapA"
                     /locus_tag="ACHUU3_000884"
     CDS             complement(40674..40982)
                     /gene="lapA"
                     /locus_tag="ACHUU3_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR2081039.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(41131..41895)
                     /gene="pgpB"
                     /locus_tag="ACHUU3_000885"
     CDS             complement(41131..41895)
                     /gene="pgpB"
                     /locus_tag="ACHUU3_000885"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR2081040.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            42065..42655
                     /gene="ribA"
                     /locus_tag="ACHUU3_000886"
     CDS             42065..42655
                     /gene="ribA"
                     /locus_tag="ACHUU3_000886"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR2081041.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLNTKAEKMGHLLNK"
     gene            complement(42719..45394)
                     /gene="acnA"
                     /locus_tag="ACHUU3_000887"
     CDS             complement(42719..45394)
                     /gene="acnA"
                     /locus_tag="ACHUU3_000887"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR2081042.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(45558..45653)
                     /gene="ymiC"
                     /locus_tag="ACHUU3_000888"
     CDS             complement(45558..45653)
                     /gene="ymiC"
                     /locus_tag="ACHUU3_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR2081043.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(45767..45934)
                     /gene="yciX"
                     /locus_tag="ACHUU3_000889"
     CDS             complement(45767..45934)
                     /gene="yciX"
                     /locus_tag="ACHUU3_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR2081044.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(45937..>46014)
                     /locus_tag="ACHUU3_000890"
     CDS             complement(45937..>46014)
                     /locus_tag="ACHUU3_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR2081045.1"
                     /translation="AWLAVFLGSALFWVVVALLIWKVWG"
BASE COUNT        10877 a        12175 c        11596 g        11366 t
ORIGIN      
        1 ccgatgatcc aataaatccg gcaacaaaca tattcgctgg ttggttataa accgttttcg
       61 gcgcaccaac ttgctgaaca atcccatcct tcataatcac aatccgcgtc gccatggtca
      121 tcgcttcggt ctgatcgtgg gtcacgtaga tcatggtggt attcagcttc tggtgcagct
      181 tgctgatctc tgcgcgcatt tgcacgcgca gcttggcatc aaggttagag agcggttcat
      241 ccattaaaaa cacgcccgct tcgcgcacaa tcgcccgccc gagcgccaca cgctggcgtt
      301 gcccgccgga aagcgcgccc ggcttgcgtt tcaggtactc acgcaggccg agaatttgcg
      361 ccgcccagtt cacccgctca tcaatcacct ctttggcgat tttttgcatc ttcaggccaa
      421 acgccatgtt gtcgtaaacc gtcatatgcg gatacaacgc gtagttctgg aacaccatcg
      481 ctatattgcg tgctttggct ggaacgtcat tcattcgttt gccgtcgatc aacagatcgc
      541 cgccgctgat ctcctcaagc ccggcaatca tgcgcagggt ggtcgactta ccgcagcctg
      601 acggaccgac aaacacgatg aactctttat cggcaatttc caggttgaag tccttcacca
      661 catgcacctg gttatcgtag attttttgaa tatgttgtaa cgaaagctga gccatgttga
      721 ttcctttgct atacgttttg ccagaaggcc gataaccgcg accaggtcag ggagtccgtt
      781 gaaggcaata gtaattgcgc cccggttatg cccgcgccga tccccaccga gcgcataccg
      841 ctggcgttaa tggcgtcaat gcccgcctgc gcatcttcaa tgccgataca tgcctgcggc
      901 ggcacgccca gcccggcaca gacagcgaga aagatttccg gatccggttt cgagttttta
      961 agttgggaag catccgcgca gaaggtgaaa aactcacgca gctccagtgc atctaaaatc
     1021 gtcggcgcat tgagggagac agaagccagc cctaccgaga tctgttgtgc gcggagatct
     1081 gccagcaaag agcgaatgcc gggtagaaca gcgttgaccg tcaactcgcg caatgagtgg
     1141 acatagagca gatttttgcg atacgccagt tgcgccctct cctgcgagtt aaagtcgccc
     1201 tctttgcccc cgtgttgcag aatgcgccgc agagactcat cgcggctgat cccttttagg
     1261 gattcgttaa actgcgcatc aatgctgatg ccaatttcag cggcaatctg ctgccacgcc
     1321 tggaaatgca gatgcgcggt atcggtgatt acaccatcca gatcgaaaat taccccttgc
     1381 agtttcattc atcctcctga tgtgtggcag cggtcccatt gaagggcaaa ataaaatcac
     1441 ccaaacagaa aacagattct tcagccacgg atataagctg accgttcaaa cgcagtgaaa
     1501 cgggggcaga agttcgaatc gcaatacgct gcgcgtcaag agtgacctgt aattcgcagc
     1561 cctgccagaa cagagggaaa gacaactgtt gccactgctc aggtaacgcc ggattgagat
     1621 gcaattcacc gtcacgcacg ctcaccccgg caaaaccctg aatcgccccc agccagatag
     1681 cgccagttgc ggcagcatgg ataccatcat cacaactatg cggatcagca ccaagatcga
     1741 tttcagtccc ctcgcgccag aactgataac tttgggtcag caggccacag cgtgcggcaa
     1801 caatgccgtg gattgcttta cttaatgacg agtcgtgaat agtgcgcggt tcataaaatt
     1861 gcagattggc aagacacgat gccgctgaga actgctccgg cagcatgtaa ttgagcatca
     1921 ccacatcagc ttgtttgagg atttgcatct cgttcacttc tgcgcgtgaa taatccagca
     1981 gaatggtttg cttccccgcc gccgctttgt atttcgcaag attaatcgcc ggcttagcca
     2041 taaacgaatc atcctgcggc aaaacgccgt cgggctgaat ttctggcagc cgcagttctt
     2101 taaggaacat ttcggcacga tgaataaacg cgtcgtcgct acagccaaac tggcgggcaa
     2161 tactcagcgc ctgctggacg ttgtaatacg ccatatagct ggtgaaggcg ttattattga
     2221 catgttcggt atattcgtca ggcccaataa catcatgaat ttccagacgg tcgttaaccc
     2281 tcaccgcgcg gctaatccag aactttgcag tttccagaag tagcgccatg ccttcatgcg
     2341 caatgaaact ttcatccccc gtggtctgcc agtattgaat aaccgcccag gcgatatcgg
     2401 ccaccagatg atgttccgcc tgcgccgagg ccactttttg ccgcagcccg gtgcgaatgt
     2461 taatggcggc aaattccggc gtctcttctt cgccgctgcg cgcgctttcc cacggaaata
     2521 gcgcgccctg ccagccgttg cgtcgcgctt tctcctgcgc gcctggcaag ttgtgccagc
     2581 gataacgcag taaacttcgg gcaaccgtcg gatcgctaaa cagatgaaac ggtaacaaaa
     2641 atacttctgt atcccagaaa acgtggcctt tgtagccttc gccggttaag ccttttgccg
     2701 caatgctgct gcgctcgtcg tgggcaggcg tcatgatgcg cagatgataa agcgcataat
     2761 ctaacgcttg ctgatcgtgc gcttcgccgc catttaccgt gatacgacgt ttctgccacc
     2821 attgacgcca gttttctgtt gatactgcaa gaagttggtc gtaactctgc tgcgcgcaca
     2881 tttcaagctg gcgaagcgac gcgctgcccc actcgtctaa aacggcttgc ctgtcatccc
     2941 gccagtcgat ccacaccagt ttttgcaacg tcactgtctc gcctgcatga agctgcgcac
     3001 tggtatgttg cagtaaacgg cgctctttgg cggtatagca ttgctgcaca tcaccgctca
     3061 ccttacagca acagctgatg gccacatcac tgcgtccatc ctgggtggtg tagatcccct
     3121 gcatcaaatg ctgaccaaac acccgcacct gggtttcgtc gagatgctgg cgaccgtggt
     3181 tggtctgcgt ggcgtcaatg cctgttgaaa tcagcactga cgcgtcggcg tccagtggcg
     3241 taatagtgat ttccagcaca atgagcggca gttggtctgc cgaaacaaag cgacggctgg
     3301 cgatggtgta acctgtgccg ttgctggtac gccagacaac gctacggcgt aattctccac
     3361 tggcaaagtc aagttcacgc tgccaggctt cgcgggataa cgagaaaacc tcaccattta
     3421 tggcaatctc catccccacg acatcaggca ggttcaccag ttcgttgatt tcaccttttc
     3481 ccgcccgatg atacagcccc gccagataca tccctcgcgt ctggcgcgtg taatcttctt
     3541 catggctggc gcgaagccca agatagccgt tcccctgcgc catcagcgat gcatacttgt
     3601 tcagggtatg ctggctgaaa tggggttctg ataacgttac tggcctggtc ataattccac
     3661 acgcgtccct gtttctgcgg attgatacaa cgccgccacc agttgctgga tgatgtaccc
     3721 ctgcttggcg tcggctatca tcacgggctt gccctgtacg tgattgataa aggcttccat
     3781 gctgcgcaaa tggcggttgt cgtctgccat tttccgttgc atcagcgtca ttaattcacc
     3841 gttgttatcg gtgtagatat gtgctggaaa cagcgtcgca ccagctttat caccacaaaa
     3901 gctgacgttc ataatcgact gttcgcggat gttgagtgca aatgacgttt ccagccacag
     3961 aatgccgccg ttatgaaatt caatggtgcc aaacagcgaa tcttcgacgc tgtaagttgc
     4021 cggatcccac tcaccaaatt gaccacagct cttttgcgtg ccgatctttt gaaagctatg
     4081 cgcattcacg cttttcaccg ccggaaagcc cagcacatac atcgcagcat ccagcatatg
     4141 aatgccgatg tcgatcagcg ggccaccacc ctgcagttct ttattggtaa agacacccca
     4201 gccgggaacg ccgcagcgac gcagggcgcg ggcggtggta acgtaaatct ctcccaaaac
     4261 gccgttggtc acctgttcac gcagctgttg cgtatcgagc gcaaaacgat ggtgaaagtc
     4321 gtaggccagc accttgccca gtttgcgcgc ggtatcgcac atttcccgcg cctgttctgg
     4381 cgtcatggcg agcggttttt cgcacatcac atggcagccc gcttccagtg ccatcagggt
     4441 atgttcgtaa tgaaaacggt taggtgagca gacgctaacc acatcaggtt tcaccgccag
     4501 cagcatggcc tgcggatcgt cccacacgga tgcattcccg tatttttctg ccagcgcctg
     4561 cgcctgggaa aggcggctgt cacagacagc caccagttcc agatcgttgc gggtgcagta
     4621 gtacgaagca tgaaccttat ccgccacctg gcctgcgcct attatcgcga cccgcagcgg
     4681 agagcttgtc attgcacttt tcacaaagac ctcttagcag gtacgcaacc aggccaacga
     4741 atcacggtac gcctgggcag gatcttccgc ccggatacgc ccttcgtaca ctacatagcc
     4801 ctgatagtta tccgcgcgca gctgttcaaa cagcgcgtgg aaatccaggg tgccgctgcc
     4861 cggctggtag cgatggttat ccgcaatatg cacatgaccg agcaggtcgc ggttgtcatg
     4921 cagcgcctgc gccaggttat cttcttcgat attcatgtga tagaaatcgc cgataatctg
     4981 tacatgttta agatcgtttt cgacgatgta acggcgggca tcggcgaggg tgttgatcat
     5041 atgatcctga tagcggttta acggttcgag atacaccact gttccggtac gcgcggcgac
     5101 ctgttccagt acgcgcaggg aatcactcac cattttgcgg tcgccgtcca ggctacgcgg
     5161 cgaggtcatc ggcggtaagc ggaaggtaaa catgccccac gctgccggaa cgacgatacc
     5221 tttatccccc acttctgcca gcgcttcgag aatgcgttcg atctgcttta agccattaag
     5281 acgacgctct tcgataaagt cgccaatcca accgtcatag ccaccgcagg cggtggtcac
     5341 cggtaaaccg gtttctttga ttgctgcttt gacttcttcg agattgttga ccagcagttt
     5401 gccgtcaatc tcaaaaccat cgaagcccat ctctttgata taacgaaatt tctccagaat
     5461 gttttccgga aagaatgcct gattctgtgt gcctattttc attttttcat tcctttgaat
     5521 taaaacgtaa cgcccatttt gatgctctgt tccggatgct gatcgacata ctgcatgtag
     5581 ctttccgggc tattggcaaa ggtcactacc gggtcgatta aatcttcgca attgagataa
     5641 ccgttcatca gcagttccca gcaggtttct tcaatacgct tacggctcca gcgcggataa
     5701 tccgggttcg gttcgctgca cgcgcgggag aaaacaattt tggcgttatt gaaatgcgct
     5761 tcgcgtccga ggttaaaacc ttcagcaaat ggtttggcaa acgcaacata ggagatggtg
     5821 ccaccgtagg ccagaccgcg gaacgccgat tgcagcgcgt cggcatagcc gctggtttcg
     5881 ataatcacat cggcaccctg cttgccggtc agcgttttga tctctttgcc tacgtcagta
     5941 ccaatggggt taaggcagaa atccgcgccg tggcgacggg caatatcaca gcgatgagcg
     6001 ataggatcga cgccaatcac gaccgacgcg ccagcacgtt tagccagttg aatggcaatt
     6061 tgaccaatcg cgccaagccc taccaccaca acaaaatcac ccacgcgcac gttggcatcg
     6121 cgcacgccgc tcatggcaaa ctgcgccggg tcgtaacaaa cggcgttttt ccacgagctg
     6181 ccttccggca ttttgcgcag cttgtagtta ttcaccgcgt taatgatgac cgtctcggag
     6241 agcgggccgt agccgcatac gctgtcgccc accgcgtagt cggtaacgtc gctgccgcac
     6301 tcgataatgt cgccaaccac catgtttcca agctggaatt tgccaaactc aatgccgcgc
     6361 ggcgcatctg ccggacgcgg agtgaacatc tgccattcgc cgttaaagtc ttcatcgata
     6421 aacgggctgg cggcgcggaa gtcgaccact tccgttccgt gtttcggtgc gccgaaacgc
     6481 acgcggacct tcacttcatt agctaaaatc gcccgatctt catactcaac cagcgcagca
     6541 acacgcggtg ctgtggctac taactttttc atttaaaact ccttgtaaag aaactggccg
     6601 ctttagccct tcacgccacc ggcggtcaaa ccactcttaa taaaacgttc cgaaagcgca
     6661 tacataatca ccaccggcaa tgcggtcacc agtgaggccg ccatcatccg tccccagatg
     6721 taatctggcg tactgaacag cgcgttcagg cccaccggta aggtgaaatt gccggcgctg
     6781 gagaggaaaa tcgacgcaaa cagatagtcg ttccacgcca ccataaagca gtagacgaac
     6841 acggatatca acccggacat cgccaacggc acggtgatgc ggaagataat ttgcagacgg
     6901 ttgaggccat ccatcatcgc cgcttcttcg atctcatcgg ggatggtgtc gaagtagctt
     6961 ttcagcataa acacggcggt gggcagagtt tgcgtcacca tggtgatgat cagcgccatc
     7021 tcagtgtcgt aaatgcccag cgcggtgatg attttgaaca gcggcaccac cagcaaaatg
     7081 ccagagaaca tatacaccgt gtaaaagctg gcgttgatgg tcatccgacc tttaaagcgc
     7141 aggcgggaaa gcgcgtaagc cccaagaatg ccgaggaata ccgccaccac ggatgaaacc
     7201 acagacacca ccagactgtt acggaagtag tcgacaaacg ggaaaatcat cgggttaaaa
     7261 atgtcgacgt aatgctccag cgtccactgc tgcggcagca gcgtaggatg cagtgagatc
     7321 gcctctttcg cgcccttgaa cgaggtcatc agcatcacaa aaaatggaaa cagcgtgatg
     7381 atgagaaaca gcgccagccc gcagtaaaaa ccgatgcgac tgagtgtgcg tttatttgtt
     7441 gccattgagg ttcacccgtt tcctggtcag cagaatgaca gcgaaaataa tgatgaagag
     7501 caccaccgaa atcgccgccg ctttccctaa gtcattaaag gcaaaggcgg ttttgtagag
     7561 atagacgccg agaatatcga ccttggtggt cagcagataa acatcagcaa acatgtagaa
     7621 catccagata gtgcgcaggg tcaccaccgt cgccaggacc ggcataattg cgggcagcgt
     7681 gacgatgcga aaccgctgcc aggcattagc gccatccatc tctgccgctt cgtacagcga
     7741 tttgtcgatg gtctgcaaaa tcgccagaaa cgagataaag gcatacggga agtagcgcca
     7801 gatggcgaac agcaccacca gcacaaagct actgcccgga ttatcgaacc acagcggtgc
     7861 ctgctcatag agatgcagaa gatcgacgcc gaggtagttc acaatgccgt agccgttgtt
     7921 gaacatgtat ttccaggcaa acaccagcga aatggacggc gttacgtagg agaggatcac
     7981 cagcgaacgc gcggttttgc gcaggcggaa ctcgcggttg aaaaacatcg ccactgccag
     8041 cccgagcacg gtgctgcccg ccaccaccag cgcggtatac cagaccgtca tccacagcga
     8101 gtgccagaag ccgggatcgg agaggatacg cacatagtta ctcaccccaa caaaggttga
     8161 ctcgatgttg ggattgagcg gcagacgtaa aaaactgatt tcgatattcg acaccattgg
     8221 ccaggccacc agaccaccca gcagtaataa gctgggcgcg agaagcagca gcgcaaaggg
     8281 catatcggaa cgacctgaaa aaagcctgtt cattgcggat tcctgtcctg ggtttagtgc
     8341 tgttcgatca gttcatccag ctttttctgg ctcgcttgca gcgtgccagg gagatcggct
     8401 ttacccacgg tgacgttatg caccatggag ctcaccacgc cagaacccgt cacatcaccc
     8461 atgcgggtaa agtttttatc ccctactgcg ccaaagacct gaatatttgg cagttcaccg
     8521 attagctgat tcggtagttc acccagcgcc ttaataacgt cgttgtcttt ccaggtggcg
     8581 gtagtgacaa ccgctttatt caccggcagc gcagcacctg gcgacatcat cacccagtcg
     8641 gcaatgttgt ctgcctgctc cataaaggtg acaaattttt ctgctgcttc cgtctcttcg
     8701 gccttttgcc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
     8761 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaattac agccggaagg
     8821 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
     8881 atgatgtcgt tagaacccgg catgtcgtta gaacccggca tagtgttggc agcaaggtcg
     8941 cggtaatagg tcagtgcctg catcatctct ggtgtatcaa gggtgatttt gccttcggcg
     9001 ttaaagacgt tagcctggtt ggataacgca aactgggaga aggattgttc cgtcaacacg
     9061 ctttctgctg taggcagcgc aatgccgtat tttttattcg ccgggtcatt cagtttctgt
     9121 gcaacgtcca gtagctgttg ccagtttttc ggctcctcca gccccgcttt tgccagcaca
     9181 tctttgcgat accagatgcc gccaatccag gcgctgacgg gaacaccggt ccaggcactc
     9241 ccatcttcgg tgcgcacaat acgcagtacg ccatcgtaaa acgcgccttc accaacgttg
     9301 ctgatgaccg tggcaaccgt cttgcgatcg ataagctgct ctttatccat aactttggcg
     9361 tagtcatggc tggtttcgat cacttccggc agcgagccgc tacgtgaaag ggtgatgact
     9421 ttggtgttat aagcgtcctc ttccacgggc acttgtttaa cggtgatacc tgggttttct
     9481 ttttcaaaac gggcaatcaa tttgctgata actgcctgac gctcctgctc caccgaagaa
     9541 tgcataaatt cgatggatac atgggtttta ttttcttctt tacagcctga aatcagggcg
     9601 caggaaacca gtgctgataa cagcacgatt tttgatttaa tcatgagatg ttccttttta
     9661 tttaatccac ataacctgct aggggcgcag cgttatttca ttacccaata gatcaacttc
     9721 actaatcaga tcccgaccat gcagattagt aatatttaca tgctgaatat ttttactgac
     9781 attaaacaat ccagtcagac aattaccatc agcgttactt ctttgaatac gcattacgga
     9841 tgaattaatc gtatcgatgg taaaattatt atccggatga aactcgttat ggctgcgacg
     9901 aagtgtaatt aaacgcgaca actcatgata taccgcatgc cttaatgtag cttcatcgtt
     9961 cagttctcgg gttatctctt tactgtgata ttttttacgg ttaatcgcac ggttatatcc
    10021 gagtttttcg acacctgcgt aatcattacg cgagccaaga atactttgaa tatatatcgc
    10081 tggcacaccg gggaaactta acaaaatcgc atgggcgaga ataaacctgg cgcaacgttc
    10141 ttcatcgcta ctctcacggc ggcttaacgc atccatatgt gagaggcgag gaagttaaac
    10201 caggtggtgt tgctggaagg tagtgtcagg ttttgcgccc acgcacaaag cgcctcaacg
    10261 ttctgttttt gcaccgcatg cagcaccagt ggcggcagcg agaactggta caccatatgt
    10321 gcttcgtcat cgcctgcgcc aaagtaagca atgttgtctt tatgcggaac attggtctcg
    10381 gtaatgatca ctgtacctgg cgcaacgtta tcaataatcg accgtaacag tttgataatc
    10441 agatgtgttt tttccagatg gatgcagctt gttcccggct ctttccacat aaagccaacg
    10501 gcatccaagc ggacatactc agcacctttc gcaaggtaac agagtaaaac atccaccatc
    10561 gccagcaaca cttcagggct acggtaattc aggtcaattt gatcgtcact aaaggtggtc
    10621 cataaatggc gcgttgaatg atcgcgcatc tggaatggcg ttaataacgg taacgcacgc
    10681 gggcgagtga cggcgctgag atcggtttgc ggatcaaccg caataaaaaa atcttcaaaa
    10741 cctggctgct gttgtaaata gtttttaaac cattcacttt ttgccgacat atggttgcag
    10801 acaaaatcaa acattaaatg actgcattca ccgagttgct gaatatcctg ccactccccc
    10861 gcttcactgg cgacctgatg ataatcaatt accgaaaacc catcatcaga tgaacatgga
    10921 taaaaaggta ataaatgaac atgtgaaaaa atgctttgca gccattgatg gtaaaactga
    10981 ttaaatgtgg gtaatggttt taaatcattg ctgtgaaatt gatcggcata ggtaattaac
    11041 acgacatcac tttcatccca gtgtttttta cgtcgctgtg taattaatcg tttcgcactc
    11101 tcaagacgcg ttacaagttt ctgtaaatga gttgcggtaa atgttccacc gtagatttcg
    11161 tccaggtaat ccgtaatttt ctgtttcatg ttgccttttt gtggtagcgg tcccatgaaa
    11221 cgagagacta ctccgcagaa aataaactgt caattgagca caagggtttt agtcacgcag
    11281 gaaatcattt tttaccaggg aaaaagcgta tttgtgagct gacgcaaata atctatgggg
    11341 atgtgaataa ggtgtgacag aaaaaaaacg gcgcataagc gccgctcatg gtgaattctt
    11401 ttaacctttg accttcggca ttgcgatgtc tttcaggcca ccggcgttct caacgtgggt
    11461 atatcccatc tcgctaagga tctcttttgc ttgccctgac tggcgtccgg cattgcaata
    11521 cactttcacg gtgtcgtttt tatccggaac ggcggtggca atgcgctctt tcacttcctt
    11581 caggggaata ttgatggccc cctgaacgtg ctcttgctga tactgctctg gaacacgaac
    11641 atcgatccag tgttcagcgg cgaaaacggg cagtgaaaac accagtgcca gagcaagtaa
    11701 gcctttttta aacatgtaag cgtccttcta tggatgaaaa ttgccatcca ttatacgcct
    11761 gagatttaac aatttttgcg aaactcccct caccttttat aacgctgtgc cagtttatta
    11821 gcagcccgac ttaacagcgg ttccagcgcc acagccagca acattttcag cggtcggcga
    11881 gcaactgatt ttatcgccca gcccgctaca cccgccgggc catagcgcag tgcggtaaga
    11941 agtaccagct tgcctgctaa tttgaaacca ggctttacct tttgcccggc ctgttgccag
    12001 cgagtattca taactttcct cacagttgac ggaaacggct acgtaacgtg aaagtatcgg
    12061 aagtgacata acgttccatc tcgcgtaaac gcgtttcact tgccgccagt tcacggtcga
    12121 cttcatccag caattcgctg ctggaaggta gctgctcacc aaaggccatg ttgtccggca
    12181 ttggatcaag cgcaaatgac aaaatgatgt aagcaaccag ggtaaacagc gccagaccga
    12241 agaaaatcga cagcaccacc aggatacgca ccagttttac cggtacatca aaatagttgg
    12301 caatcccggc gcagacgccg cggaccatgc cctgctgtgg aatacgccat aattttttat
    12361 tgagattaat gcccgccatt agcgatccct ccagttcgga tgttctgcat caagaataga
    12421 ttccagcgcc tgaatacgtt cgcgcatccg ttttgcttca tcagccagtt gcgctaatcg
    12481 ctgctgctca ctttgcgaca attcatcgcg accagaacga ttgctgtaat gcagccataa
    12541 ccagatcggt aaaacaaaca gcacaaaaat ggttaacgga atagccagaa atagcgcgct
    12601 cataagtact ccttagacga tgagcggcgg cgcgttcgga cgccgcctgg atgttattat
    12661 tgattgtctt gcttcatttt ggcttttaat tgtgccagtt gttcgctgat tgcatcatcg
    12721 gctttcagtt cggcaaactg atcgtccagc gatttttgtt taccgaagct gtggctttct
    12781 gcttccgctt ccatctggtc aattcgacgt tcgaaagatt cgaaacgagc cattgcttca
    12841 tccagtttgc cactgtccag ctgacgacgc acatcgcgcg acgagtttgc cgcctgatga
    12901 cgtaacatca atgcctgctg gcgagcgcgt gtttcgctca atttgttttc cagctcgcca
    12961 atctctttct tcatgcgtgc cagcgtatcg tccaccagcg tcacttcatg ttccagggac
    13021 ttaatcagat cggtcagttt ctgtttttca attaacgctg cacgtgccag atcctctctc
    13081 tctttcagca gcgccagttc ggctttttcc tgccattcaa cttcacgcgc cgacgcttgt
    13141 tcaatacggc gagtcagctg tttcttttct gccaacgcac gcgccgaagt agaacgtact
    13201 tcaaccagtg tatcttccat ctcctggatc atcagacgca ccagtttctg tggatcttcc
    13261 gctttctcta acagagcgtt gatgttggca ttcacgatgt cggcaaagcg agaaaaaata
    13321 cccataatgt tgtcctcttg atttctgcgt tcaggattgt cctgctgaac tgttaataca
    13381 atttgcgtgc caatttttta tctttttgat ttataaagat ctgattgaag aatcaacagc
    13441 aacatgccag gatgaattag cgaattacac taacaagtgg cgaatttcat catggccgaa
    13501 tacaaagata atttacttgg tgaggcgaac agctttctcg aagtgctgga acaggtttcg
    13561 catctcgcac cgctggacaa accggtgctc atcatcggcg aacgcggcac cggtaaagag
    13621 ctgattgcca gccgcctgca ttatctctcc tcccgttggc aagggccgtt tatttccctt
    13681 aactgcgcgg cgttaaatga aaatctgctg gattccgaac tgtttggtca cgaagcgggg
    13741 gcgtttaccg gtgcgcaaaa acgtcatcca gggagatttg aacgtgccga cggcggtacg
    13801 ctatttcttg atgaactcgc tacggcaccg atgatggtgc aggagaaatt attgcgcgtg
    13861 attgagtacg gtgaactgga gcgcgttggc ggcagccagc cattgcaggt gaatgtgcgg
    13921 ttggtatgcg cgacgaatgc cgatctcccg gcgatggtca atgaaggcac ttttcgcgct
    13981 gacctgctcg accgactggc ttttgatgtt gtacaactgc caccactgcg cgagcgcgaa
    14041 agcgacataa tgttgatggc agaacacttt gccatccaga tgtgtcggga aatcaagctg
    14101 cctctgttcc cggggtttac ggagcgcgcc agagaaacat tgctgaatta tcgttggccg
    14161 ggaaatattc gtgaattgaa aaacgcggtg gaacgttcag tgtatcgcca cggcaccagc
    14221 gattatccgc ttgatgacat cattattgat ccctttaaac ggcgtccgcc tgaagacgct
    14281 atcgccgttt cagaaaccac ctcgcttccg acgctgccgc tggatttacg tgagtttcaa
    14341 atgcagcagg aaaaagagtt gctgcaactc agtttgcaaa aggggaaata taaccagaag
    14401 cgcgcagctg aattgctggg gttaacctac catcagttcc gcgcgttgtt gaaaaaacac
    14461 cagatttagc gaagttgggc aatgcgattc gccggatgcg gcgtgaacgc tttatccggc
    14521 atacataatc aacgtcgttt gcaggcctga taagcgcagc gcatcagaca ttattgcgtt
    14581 gggctattaa tcgctcagcg catcctgcaa aattttcatt gccgcatcga attgcgcatc
    14641 cgggatggtc agcggataca ggaagcgaat cacgttgccg tatgcgccac aggtcagcag
    14701 gagcagcccc tgcgccagcg cgcgttgctg gattttctgt gcaatcgccg ctgacggctc
    14761 gcccgtttgc ggatcgttaa actctgccgc aatcatcgcc cccaggccgc gtaccgcagc
    14821 aatggccgga acgctttctt tggcatcaat caacgtgttt ttgagacgct ggcccagttg
    14881 attcgcgcgt tcgcagagtg attctttgtt gataatattg agcaccgcgt gcgcggcagc
    14941 caccgccagc gggttcccgg cgtaggtgcc acccaacccg cccggcgcgg gcgcgtccat
    15001 aatattcgcg ttaccgacca cgcccgaaag cggcatcccg cccgcgaggc ttttcgccat
    15061 cgtcattaag tccggcttat cggcgtaatg atccatggca aacagtttac cggtacgcgc
    15121 aaagccgctt tgcacttcat cggcaatcat cacaataccg tgctcgtcgc acaggcggcg
    15181 aatggcggca accagctctt ttggcgcaac gttgaaaccg ccctcgccct gcaccggttc
    15241 gaaaataatg gccgcgacct gcttcgcttc gatgtctgat ttaaacaagc gttcgatggc
    15301 gtcgagggag tcctatgttg aaatgccgtg taaatctgac ggataaggta cgtgatacac
    15361 cgaaccaggg aacgggccga agccgatttt gtacggcgca acttttccgg tcagcgccat
    15421 ggtcatatat gtacgaccgt ggaagccacc gctaaacgca atcacgccag ggcgtccggt
    15481 atgggcgcga gcaattttca ccgcgttttc caccgcttcc gcaccggtag tgaagaacgc
    15541 ggttttggcc tgcccgctca ccggggcaag ggcgttgatt ttctccgcca gggtgacgta
    15601 gctttcatac ggcacaatct gatacgcggt gtgggtaaac tgttgcagtt gctgctccac
    15661 cgccgcgacc agatcagggt ggcgatgtcc ggtattcagc accgcaatgc ctgcggcgaa
    15721 atcgatgtac tcgttgccct caacatcctt cagagtagcg ttttcagccg actgggcgaa
    15781 gaagttacac atcacgccaa ccccgcgtgg ggtagcagaa agacgacgct gatggaattc
    15841 attgttgctc atgtttcaca ccttttgact cttcactgtt aatcggaaat taaacgccca
    15901 gacgatcgcg caggctgtac caggctgcgc ccatcgcggt aaacggtact cgcagcgtgc
    15961 gcccgccggg gaacgggtaa tacggcagat tggcgaaggc atcgaaacgt tcggcgtcgc
    16021 cgcgcagcag ttcggcaatc aaacgtccgg cgagatgagt acaggtgacg ccgtggccgc
    16081 tgtaaccctg catgtaatag atgttggtat caaggcgacc aaactgcggc atgcgcgaca
    16141 gggtcagcag gaagttgccc gtccagcggt agtcgatttt gacgcccttc agttgcggga
    16201 aggttttcag cagtttcggc acaacaagac gctcaacgtc atccgggtcg cgcgcgccgt
    16261 agaccacgcc gccgccgtac agcaaacggt tgtcggcggt aagacggtag taatcaagca
    16321 gataattaca gtcttccaca cagtagtttt tcgggatcag cgaacgggct aaatcttccg
    16381 acagccgttc agtggtgatc acctgcgtgc cgcacggcat gctgcgtttc gccagttccg
    16441 gctctacttt atcgcccaga tacgcattcc cggcgacaat cacatacttc gccgtcacct
    16501 gacctttggc agtgcgcacc accgctggcg tggtgtgctg gatctgcgtc acggcagaaa
    16561 gctcatacac gcgcccgccg ttcaggcgga tggcatcggc ttcaccaatg gcgaggttta
    16621 gcgggtgaat atgcccaccg ctgtgatcca acagtgcgcc agtgtagcga tcgctggcga
    16681 cctcgcggcg aatggcgtta gcgtccagca gctccagctg tttattgccg tagcgttccc
    16741 agttctcttt ctgctcttcg agtgtggcga gctgtttatc attcatcgcc acaaacaggc
    16801 cgccggggcg gtagtcgcag tcaatctgat agcgtttgat acgttcgcgg atgatctcgc
    16861 caccctcgaa catcatgctg ccgagcatac gggcggtgtc catgccgtag cttttttcga
    16921 tcacgtcgat gtcgcggctg taggagttca caagctgccc gccattgcgc ccgcttgcgc
    16981 caaagccgat gcgtgaggct tcgagaacca ctacgtcaaa gcccgcttcc gccagatgca
    17041 gcgcggagga gagcccggta tagccgccgc caaccacgca aacgtcgcag gtgattgact
    17101 cattcagtgt gtcgaatggt gcatatttat tcgcactggc ggcgtagtaa ctgctggtat
    17161 gttcggtcat ttcaggcctc caggcttatc cagatggttt tcagttcagt gaatttttca
    17221 agggcatgca gggatttgtc gcgaccgttg ccgctctgct tatagccgcc aaacggcacg
    17281 gtcatatcgc cgtcgttgta gttattgacg aagacggaac cggctttcag gcggcggctc
    17341 atacggtgcg cgcgggagag gtcgcgcgtc cataccgccg cgccaaggcc gtactggctg
    17401 tcgttggcaa gctgtagcgc ctggtcttct gatgtgaaac gcgtgaccac cagcaccgga
    17461 ccgaaaatct cttcgcggct taaggacgca ttcgggtcaa catccacaaa gatggtcggg
    17521 ccgatggcgg cagccagctc agcgttacga ccatccagca gcagttgccc tttgctttcg
    17581 ccttcacgaa taaagctatg gaccgagtcg gcgtgggcgc aatcgattaa ggtgcccatg
    17641 gtggttacgg tatcaagtgg atggcccggc tgccagtttt gcgcctgctg ttttaacagg
    17701 gctaagaatt catcggcgat gctctcttcc agtaacaaac gcgttccggc gatgcacacc
    17761 tggccctggt tgtagaagat gcctgcggcg gtggcgcttg ccgctttttg cagatccgga
    17821 caatcggcga aaacgatgtt ggcgcttttg ccgcccgctt ccagccagac gcgtttcatg
    17881 ttgctatcac ccgcgtcttt cagcagttgt ttacccgtac gcgtggagcc ggtaaaggcg
    17941 atggcatcga tatcgttatg acgcgaaagc gcctgcccgg cttcatgacc ataaccgctc
    18001 actacgttca gcacgccatc cggcagcccc gcttcttctt ttgccagccc ggcgagacga
    18061 atcgcactga gtggtgattt ttcagacggt tttagaatca cgctgtttcc cgccgccagc
    18121 gccgggccga gtttccagca agtcagcagc agcgggaagt tccacggcac gatggcggca
    18181 atcacgccga ccggttcacg cacgatcatc gccagctcat ggctactggt ggtcgccact
    18241 tcgccataca ctttgtcgat cgcttcggcg taccagcgaa tggcgcgcgc cgcgccggga
    18301 atatcatcac gcagactgtg acgaatcggt ttgccggtgt cgagagtttc cagcagtgcc
    18361 agctcttcgg cgtgggcttc cattaaatcg gcgagtttat tcagtaccgc tttacgttta
    18421 gccggagaag agagtgacca gtcgccgcgt tcaaatacgc cgcgtgctgc gctcaccgca
    18481 tggtcgatat cgacgctctt gccgcgggca attttcgcca gcggtgcctg ggtgaccgga
    18541 tcaacggttt caaaggtttc attttccgcc gcagcagtat attcaccgtt aataaataag
    18601 cggttttcaa tggcgagact taacgcttta tcctgccagt aagccagatg atgaaaattc
    18661 attatgactc ctgtttcacg tctatcagat atatgcagtt tgtgaacgtc agggcgcaga
    18721 gatccccggg ccgcatgccc gtttatttct ggcgacattc tttatggatt gccgggcgca
    18781 ttgcgcttac ccggcttaca gaacaaaaag attaaaacgt ggtgggcgta tgggcgctga
    18841 taattcggca aatacctgcc gacgtattac tgaaactgtg cgggatgccg gtattaatgg
    18901 cataactttg ccccgcgacg aggtggtaat cctgaccatt aatcgtcaga acaatttcac
    18961 cttccagtac tgtgcctatt tcctcaccct gatgcttaat tctttcccca gtggttgtgc
    19021 ccggctggta cgtttcaaag atcatcgcca gcgtgcgatt cgggttaccg ttatgaacca
    19081 gcttcattga cacaccctga ctgcccatct caattaagtc gtcctgatta atgacgacct
    19141 gcggctcatc aggtttttcc ggctcggaaa agaattccga gagtgacaga ccatacacct
    19201 tcagcagctt ttgcagcgta ctgatggcag ggctgacttt atcttgttct atcgtactga
    19261 tagcactgtg agtcagcccg gagagttcgg cggcacgacg ttgtgaaagc ccctgctgct
    19321 ggcggatttc cgacaagcgt tttcctggcg ccagtccctc atcactcata tttgcatttc
    19381 cttaaactgt agtggtcaga gtcgctgttt ttcagcgata tggtgctgac aagcggtgat
    19441 aaagccctcg aacaatatac gcgaaagcgc gtactcgcta ctgttccatt ccgggtgcca
    19501 ctgtacgccc agcgcaaaag gatgattgat gacgctaacc gcctcaacta agccatccgg
    19561 cgagcgagct tcaacacgca accgtgggct aacgaccttc gccccttgtc catgtagaga
    19621 gtttacccaa aagttgctac attcaggtaa caacgcagac agtaatcccc cctcttcaac
    19681 ctgaacttca tgcgacggtg catattgctg ttccaccggc agttcgggat cttcccgatg
    19741 ttccagcaat tcaggctgtt cgcacagctt gcgatgcaac gaaccaccgg ttgccaccac
    19801 cagttcttgt aaaccccggc agatggcgaa aatggggatg cgcctttcga gtgcggcatt
    19861 aattatcgcc atgctcagaa gatcacgccc gggatcggcg tcaggctcat cgccgttttc
    19921 accatatagg tgcggctgca cattgctggg actaccagga agataaatgc catcgagttt
    19981 cggcaaaagt tgttcaagta acgacggttc cgccagcgca tgtggtagcg caattggcaa
    20041 gccgcctgcg tggatgatgg cattcaggta cttttcttgc agagtctggg tcgcatgacc
    20101 cttaagcctg ttcctgcaca ttacgacacc gataaccgga ttgttcatta tattttccat
    20161 gctcaatctc acaaagtgga ctaaattatc gccattactg cgctttaaat gacctttatg
    20221 ttcaatattt tttcaatcta gcagtgggtt ttcatttttg caaactcaat ttaacatttg
    20281 acaaacattt agtttgcata cagattcgaa tggtggtcat tatattttac gctttgataa
    20341 cgagcggaaa acaaaccaaa ggcgaagaat catggaaacc aatatcgttg aagtagagaa
    20401 ctttgttcag cagtcagaag agaggcgggg tagcgccttt acgcaggaag tgaaacgcta
    20461 cctggagcgc tacccgaata cgcaatatgt tgatgtttta cttactgatt tgaatggttg
    20521 cttccgtgga aaacgtatcc ctgtttcaag cctgaagaag ctcgagaaag ggtgttattt
    20581 cccggcctcg gtatttgcaa tggatattct gggcaacgta gtagaagaag caggtctggg
    20641 tcaggaaatg ggcgagccgg atcgtacctg tgttcctgtt ctcggttcct taactccttc
    20701 tgccgccgat ccagagttta tcggtcagat gctcctgacc atggtcgatg aagatggcgc
    20761 tccctttgac gttgagccgc ggaacgttct taaccgcctg tggcagcagc tgcgccagcg
    20821 cgacgctgaa gggaatctgc aaccgccctg cgcgcctggc accgatgacc gcaatacaca
    20881 aagccaggtt tactccgttg ataacctcaa ccacttcgcc gacgtgctca atgatattga
    20941 tgaactggcg cagttgcagc tgattccggc agatggcgcg gtcgctgagg cctcgccggg
    21001 tcagtttgaa atcaaccttt accatactga taacgtgctg gaagcctgcg atgatgcgct
    21061 ggcactaaaa cgcctggtgc gtctgatggc aggaaagcat aagatgcacg ccacttttat
    21121 ggcgaagccg tatgaagagc acgcgggcag cgggatgcat atccatatca gtatgcaaaa
    21181 taatcgtggc gagaatgtgc tttctgacac agaaggcgaa gattcgccgc tgctgaaaaa
    21241 gatgctcgcc gggatgattg acctgatgcc gtcgtcgatg gcgttgctgg caccaaacgt
    21301 gaactcgtat cgccgcttcc agccgggaat gtatgtgccg acgcaggcgt cgtggggcca
    21361 taacaaccgc accgtcgccc tgcgtattcc gtgcggcgac cgtcataatc accgcgtgga
    21421 atatcgcgtg gcgggtgccg atgccaaccc atatctggtg atggcagcga tttttgccgg
    21481 tattttgcat ggccttgata acgagctgcc gttgcaggaa gaagtcgaag gcaacgggct
    21541 ggaacaggaa ggcttaccct tcccgattcg ccagagcgat gccctgggtg agtttatcga
    21601 gaatgatcac ctgcgccgct atttaggcaa acggttttgc catgtgtatc acgcctgtaa
    21661 aaatgatgag ctgttgcagt ttgagcgcct catcacagaa acagaaattg agtggatgtt
    21721 gaaaaacgcg taattcccct tgcggggttg tttcatcggc ctgagcggag ccaggcctga
    21781 catttgctgg taatccagac atttcccgga tgtcgcgtta tggagcgcag gcggcaacgg
    21841 gcttccgtta acgattaaga ctgtgacgag gaaatgagat gatgccgatg attaaatccc
    21901 cgcatggcga gggaggctgc gtatgcgccc ctcctgctac tgactggact ccgccccctt
    21961 tgcttccctt gctaaacagg tttgatttca ggtcgacacg accgcaaacc ttattacgca
    22021 ggggaggcag caattatggc tattaattca ccactgaata ttgctgcgca acccggcaaa
    22081 acccgtctgc gaaaatcact gaaattgtgg caggtggtaa tgatgggtct ggcctatctc
    22141 acgccgatga ccgtatttga tacttttggt attgtctccg gcattagcga cggtcacgtt
    22201 ccggcgtcct atttgctggc gctggcgggc gtgctgttta ccgctatcag ctacggcaaa
    22261 ctggttcgcc agtttccgga ggccggttcg gcctatacct acgcgcaaaa gtcgattaac
    22321 ccgcacgtcg gatttatggt cggctggtca tcactgctgg attatctctt tttgccaatg
    22381 atcaacgttt tgttggcgaa aatctatctc tccgccctct tcccggaagt gccgccgtgg
    22441 gtgtgggtgg taaccttcgt cgccatttta accgccgcga atctgaagag cgtcaacctg
    22501 gtcgctaact tcaataccct gtttgtactg gtgcaaatct ccatcatggt ggtgtttatc
    22561 ttcctggtgg ttcagggact gcataaagga gaaggcgttg gcaccgtctg gtcacttcag
    22621 ccgtttatca gcgagaacgc gcacctgatc ccgattatta ccggtgcgac gattgtctgt
    22681 ttctcgttcc tcggtttcga tgcggtgacc acgctttcgg aagagacgcc ggacgccgca
    22741 cgcgtgatcc cgaaagccat cttcctgacg gcggtatacg gcggcgttat ctttatcgcg
    22801 gcgtcgttct ttatgcagct gttctttccc gatatcagcc gctttaaaga cccggacgcc
    22861 gcactgcctg aaattgcgct gtacgtcggc ggcaagctct ttcagtcgat tttcctctgc
    22921 accacgtttg tgaacacatt agcgtctggc ctggcttcac atgccagcgt gtcgcgtctg
    22981 ctgtatgtga tggggcgtga caatgtgttt ccggagcgcg tgtttggcta tgtgcaccca
    23041 aaatggcgga ctccggcact caacgtcatt atggtcggga ttgtcgcgct gtcggcgctg
    23101 ttctttgatt tagtcaccgc gacagcactg attaacttcg gtgctctggt ggcgtttacc
    23161 ttcgtgaatc tgtcggtgtt taatcatttc tggcggcgta aaggaatgaa taaaagctgg
    23221 aaggatcact tccactattt gctgatgccg ctggttggcg cgctgacggt gggtgtgctg
    23281 tgggttaacc tagagtcaac gtcactgaca ctcggtctgg tatgggcttc gttaggcggc
    23341 gcatatttgt ggtatctgat ccgccgctat cgcaaagtgc cgctgtacga aggtgacaga
    23401 acgccggtga gcgaaacgta atagccggat gcgcggcgca ttatcctgcc tgagggtaac
    23461 gcgccgcgcc attaactata aaaagatgta tgacccggat cacgagtgct atcatccgcc
    23521 cttatgctta gcgcattaac cccaggggca aataatgaac cacgatatcc cgctaaaata
    23581 ttttgatatt gccgatgagt acgcgaccga atgcgcagag cctgtcgcag aggctgaacg
    23641 cacaccgctg gcccactact tccagttact gctcacccgt ttgatgaaca atgaagagat
    23701 cagcgaagaa gcccaacatg aaatggctgc tgaagcgggg attaatcccg tacgcattga
    23761 tgagatcgca gagtttttga atcaatgggg taatgagtaa ttatttaatt gcagacgatc
    23821 ggtcagtatc gttgtccgga taaagcgttc acgccgcatc cggctaacaa tgacacgcac
    23881 tgcagcgtgc tttaatgggt ttctcacgat cgtaccgctc tgctcctcgc taagttgttc
    23941 tgtgagttat agttatagag aaatcacggg tacgaatgtt gaacagatgg cgacgccacc
    24001 tgttcaacga ccaaccacac ggccctcatt acgacccctg aacacaaacg gtaatggcaa
    24061 atgtcattaa atattagtaa gatagcctct ttacataaaa tcccttaata ttatgcgcca
    24121 ggtattatcg tctcttttgg tgattgctgg acttgtgagt ggtcaggcaa tcgccgcgcc
    24181 tgaatccccc ccgcatgctg atatccgcga cagcggtttt gtctattgcg tcagcgggca
    24241 agtcaacacc tttaacccat ccaaagcgag cagtgggtta attgtcgata cccttgccgc
    24301 ccagttttat gatcgactgc tggatgtcga tccctatacc tatcgcctga tgccggaact
    24361 tgccgaaagc tgggaagtac tcgacaacgg cgcgacctat cgcttccacc tgcgtcgcga
    24421 tgttccgttt caaaaaaccg actggtttac tcccactcgt aaaatgaatg ccgacgatgt
    24481 ggtgtttacc ttccagcgaa tttttgaccg caacaacccg tggcataacg tcaacggcag
    24541 caacttcccc tacttcgaca gcctgcaatt tgccgataac gtgaaaagcg tccgcaaact
    24601 ggataatcat accgttgagt tccgactcgc ccagccggat gcttcttttt tgtggcacct
    24661 cgcaacccat tatgcttcgg tcatgtcggc agaatatgcc cggaagttag agaaagaaga
    24721 tcgccaggag caactcgacc gtcaaccggt cggcaccggt ccgtatcagt tgtcggaata
    24781 ccgcgccggg caatttattc gcctacaacg tcatgatgac ttctggcgcg gtaaaccgtt
    24841 aatgccgcag gtagtggtgg atttaggctc cggcggcacc ggacgtctgt cgaaactcct
    24901 gaccggggaa tgcgacgttc tggcctggcc tgctgccagc cagctatcca ttttgcgtga
    24961 cgacccgcgc ttgcgtttaa cgctgcgtcc tgggatgaac gtcgcctatc tggcatttaa
    25021 caccgccaaa ccgccgctaa ataatcccgc tgtccgccat gcactggcac tggcgattaa
    25081 taaccagcgc ctgatgcaat ccatctatta tggtacggct gaaacggcgg cctctatttt
    25141 accgcgcgcc tcgtgggcct atgacaacga ggctaaaatt actgaataca atccggcgaa
    25201 atcgcgcgaa cagttgaagg cgttggggct ggaaaactta acgctgaaac tgtgggtgcc
    25261 cacacgttcg caggcgtgga accccagtcc actgaaaact gccgaactga ttcaggcgga
    25321 tatggcgcag gttggcgtaa aagtggtgat tgtgccggta gaaggtcgct ttcaggaggc
    25381 gcggttgatg gatatgagcc atgatctgac gttatccggt tgggcgacgg acagtaacga
    25441 cccggacagt ttcttccgtc ctttactgag ctgcgcggca attcattcac agaccaacct
    25501 cgcccactgg tgcgatccga aattcgacag cgtgttgcgt aaggcgctct cctcgcagca
    25561 gctggcggcg cgtattgaag cctatgacga agcgcagagt attctggcgc aggaattacc
    25621 tattctgccg ctggcgtcgt cattgcgttt gcaggcctat cggtacgata tcaaaggtct
    25681 ggtacttagc ccgtttggta acgcctcctt tgctggggtg tatcgcgaga aacaggatga
    25741 ggtgaaaaaa ccatgattat cttcacctta cgccgcattt tgttattgat tgtcaccttg
    25801 ttcctgctga ccttcgttgg cttcagcttg agctatttca cccctcatgc gccgttacaa
    25861 ggcgcgtcac tgtggaatgc ctgggtgttc tggtttaacg gcctgatcca ctgggatttt
    25921 ggtgtttcca gcattaacgg tcaaccgatt gccgaacagt taaaagaggt cttcccggcg
    25981 acgatggaac tgtgcatcct cgccttcggt tttgcgctga ttgttggcat ccccgtgggg
    26041 atgattgccg ggattacgcg ccataagtgg caggataact tgatcaacgc catcgcctta
    26101 ctgggctttt caatcccggt gttctggctg gcgcttctgt tgacgctgtt ttgttcactc
    26161 acgctaggtt ggttgcccgt ttcagggcgt ttcgatctgc tctacgaagt gaaaccgatt
    26221 accggttttg cgttgattga tgcctggctt tcggactcac catggcggga tgaaatgatc
    26281 atgagcgcaa tccgccatat gatattgccc gtgatcaccc tgtcggttgc gccaacaact
    26341 gaagtgatcc gcctgatgcg tatcagcact atcgaagtgt atgaccagaa ctatgtaaaa
    26401 gcggcggcga cccgcggttt gtcgcgcttt actattttgc gtcgccacgt tctgcataac
    26461 gcgttaccgc cggttattcc tcgcctgggc ttacagtttt ccaccatgct gacgctggcg
    26521 atgatcaccg aaatggtttt tagctggccg ggtttagggc gctggttaat taacgccatc
    26581 cgccagcagg attacgcagc catttccgcc ggagtgatgg tgtgtggctc actggtcatt
    26641 attgttaacg tgatttctga tattttgggt gccatggcta accctctgaa acataaggaa
    26701 tggtatgcct tacgatagcg tatacagcga aaagcgcccg ccgggcacgc tgcgtaccgc
    26761 ctggcgcaaa ttttatagtg acgcctctgc gatggtcggc ctgtacggct gcgcggggct
    26821 ggctgtactg tgtatttttg gcggctggtt tgcgccttac ggcatcgacc agcaatttct
    26881 cggttatcaa ttattgccgc catcatggtc gcgctatggc gaagtttctt tcttcctggg
    26941 gactgacgac ctcggacgcg atgttttaag ccgtttactg agcggagctg cgccaaccgt
    27001 gggtggcgca tttgtggtga cgcttgccgc gacgatctgt ggcctggtac tgggcacctt
    27061 cgccggggcg acacacggcc tccgctcagc ggtgcttaac catattctgg ataccttact
    27121 ggcgatccct tcgctgctgt tggcaattat cgttgtagcg tttgccggac cgagtttgtc
    27181 ccacgctatg tttgctgtct ggctggcatt gctgccgcgt atggtacgtt cgatttacag
    27241 catggtgcat gacgaactgg aaaaagagta cgttatcgcc gcccgtctgg atggcgcatc
    27301 aacgctgaat attctctggt ttgctgtgat gccaaacatc accgccgggc tggtcaccga
    27361 gatcacccgc gcactgtcga tggcaattct cgatatcgcc gcgctgggct ttctcgatct
    27421 cggcgcacaa ctcccctcgc ctgaatgggg agcgatgctc ggtgatgcgc tggaactgat
    27481 ttatgtcgcc ccgtggactg tcatgctgcc aggtgcggca attatgatta gcgtgttgtt
    27541 ggttaacctc cttggtgatg gcgttcgtcg tgcgattatt gcgggggtgg aataatgcca
    27601 ttactcgata ttcgtaacct gaccattgaa tttaaaaccg gtgatgagtg ggttaaagcc
    27661 gtcgaccggg taagtatgac cttaaccgaa ggtgaaatcc gcggtcttgt tggtgaatcc
    27721 ggttccggca agagtttgat tgcgaaagcg atttgtgggg tgaataaaga taactggcgt
    27781 gttactgctg accgtatgcg ttttgatgat atcgatttgc tgcgtctctc cgcacgcgaa
    27841 cggcgcaaac tggttggcca taacgtgtcg atgattttcc aggaaccgca gtcgtgtctt
    27901 gacccttcag aacgtgtggg tcgccagttg atgcaaaaca tcccagcctg gacctataaa
    27961 ggccgttggt ggcagcgttt tggctggcgc aaacgccgtg ccattgaact gctgcaccgc
    28021 gtggggatta aagatcacaa agatgcgatg cgcagttttc cctatgagtt gaccgaaggt
    28081 gaatgtcaga aagtgatgat agccattgca ctggcgaatc aaccgcgtct gctgattgct
    28141 gacgaaccga ccaactcaat ggagccaaca acccaggcgc aaatctttcg cctgctgacg
    28201 cgtctcaacc aaaacagcaa taccactatt ttgcttatca gccatgactt acaaatgctt
    28261 agccaatggg cggataaaat taacgtgctt tactgcggtc aaacagtgga aaccgcgccg
    28321 agtaaggagt tggtgacgat gccacatcat ccttataccc aggcgctgat ccgcgcgata
    28381 ccagacttcg gcagcgcgat gccgcataaa agtcgcctca atacgctgcc cggcgctatc
    28441 ccactgctgg aacagttacc gattggttgt cgtctggggc cacgttgccc gtatgcacaa
    28501 cgagaatgca ttgtgacgcc acgtttgacg ggggcgaaaa atcatctcta tgcctgtcat
    28561 ttcccgctga acatggagaa agagtgagat gatcgaaacg ctgcttgaag tgcgtaattt
    28621 aagtaaaacg ttccgctacc ggaccggctg gtttcgtcgt cagaccgtag aagcggtaaa
    28681 acccttgagc tttacgctac gtgaaggcca gacactggcg attattggcg agaatggttc
    28741 gggtaaatcc acgctggcaa aaatgctggc gggaatgata gagcctacca gcggcgagtt
    28801 attgattgac gatcatccac tgcattttgg cgattattcc ttccgcagtc agcgtattcg
    28861 catgattttt caggatcctt cgacctcgtt gaatccccgt caacgtatct cgcaaattct
    28921 ggattttcca ctgcgcctga acaccgacct ggaaccggaa cagcgtcgca aacagattat
    28981 tgaaacgatg cgtatggttg ggctgctacc ggatcacgtc agttactatc cgcatatgtt
    29041 ggcacccgga caaaaacagc gtctgggtct ggcgcgcgcg ctaatattgc gcccaaaagt
    29101 cattattgcc gatgaagccc tcgcttcact ggatatgtcg atgcgttcgc agttgattaa
    29161 tctgatgctg gagttacagg aaaaacaggg catttcgtat atttatgtta cccagcatat
    29221 cggaatgatg aagcacatta gcgaccaggt gctggtgatg catcagggcg aggttgtcga
    29281 gcgaggcagc accgcggatg tgctggcatc gccgctgcat gaactcacca aacggctgat
    29341 tgccgggcac tttggcgaag cattgacagc ggatgcgtgg cgtaaagatc gctaattttc
    29401 tgaatcctcc ttccagatta tccacctcat tctgacaaga gattaaatct tgtcagaatg
    29461 aggtgatgga taaaataaaa tcaaatgcgc gtgatttaag acgcaatctc actttgcagg
    29521 aacgaaagct ctggcgatat cttcgcagcc gacgttttgg tgatttcaaa tttcgccgtc
    29581 aacatccggt ggggagctac attctcgatt ttgcttgctg ctcggcgcgc gtagtcgttg
    29641 agctggatgg tgggcagcat gatttggcag ttgcctatga taccaggcgc actagctggc
    29701 ttgagtcgca gggctggacc gtgctgcgtt tctggaataa cgagattgat tgtaatgagg
    29761 aggcggtgcc ggagattatt ctgcaggaac tgaaccgccg atcaccctca ccctgaaagg
    29821 gcgagggggc cgaccgagcc gaatagctgt tgtggtgaaa acatggagac agtgctggag
    29881 aatattcggc aaggtctgaa ccatcccagc catcgccata aaagggttag gggctgtatg
    29941 agcctgtttg ttgctggggt aacaatattt gcacaatatg gtcccctcgc ccctctgggg
    30001 agagggttag ggtaagggga aaagcgccca ccctgccgca gcctgctccg gtcggacctg
    30061 gcaactatag ctactcacag ccaggttgat tataataacc gtttatctgt tcgtactgtt
    30121 tactaaaacg acgaatcgcc tgattttcag gcacaacaag catcaacaat aaggattaaa
    30181 gctatgggtt ttctttccgg taagcgcatt ctggtaaccg gtgttgccag caaactatcc
    30241 atcgcctacg gtatcgctca ggcgatgcac cgcgaaggag ctgaactggc attcacctac
    30301 cagaacgaca aactgaaagg ccgcgtagaa gaatttgccg ctcaattggg ttctgacatc
    30361 gttctgcagt gcgatgttgc agaagatgcc agcatcgaca ccatgttcgc tgaactgggg
    30421 aaagtttggc cgaaatttga cggtttcgta cactctattg gttttgcacc tggcgatcag
    30481 ctggatggtg actatgttaa cgccgttacc cgtgaaggct tcaaaattgc ccacgacatc
    30541 agctcctaca gcttcgttgc aatggcaaaa gcttgccgct ccatgctgaa tccgggttct
    30601 gccctgctga ccctttccta ccttggcgct gagcgcgcta tcccgaacta caacgttatg
    30661 ggtctggcaa aagcgtctct ggaagcgaac gtgcgctata tggcgaacgc gatgggtccg
    30721 gaaggtgtgc gtgttaacgc catctctgct ggtccgatcc gtactctggc ggcttccggt
    30781 atcaaagact tccgcaaaat gctggctcat tgcgaagccg ttaccccgat tcgccgtacc
    30841 gttactattg aagatgtggg taactccgcg gcattcctgt gctccgatct ctctgccggt
    30901 atctccggtg aagtggtcca cgttgacggc ggtttcagca tcgctgcaat gaacgaactc
    30961 gaattgaaat aatcattctg ttggtaaata ttgggcggcg ttctgccgcc cgttatctct
    31021 gttatacctt tctgatattt gttatcgccg atccgtcttt ctccccttcc cgccttgcgt
    31081 caggataacg atttccttta cgaccaagga gcgcccatgg aacaacgcca catcaccggc
    31141 aaaagccact ggtatcatga aacgcaatcc agtactgcgg agtatgacgt tctgcctctg
    31201 gtcccggaag ccgcaaaggt cagcgatccc tttctgctcg acgtgatcct tgatgaagaa
    31261 acgctggctc ccttcctttc atggctggtc cctgcgcgcg ttcttgcagt ggaattgttc
    31321 cctgaccagc ttaccgtgac ccgttcacag accttcaccg cttatgaacg cttgtctacg
    31381 gccctgacgg ttgctcaggt ttgcggcgtc cagcggttat gtaactacta ttcggcgcga
    31441 cttacgccgc tccccgggcc tgattcctcc agggaaagca atcataggtt ggcacaaatc
    31501 acgcaatatg cccgccaact ggctagctcg ccttctatta tcgacaaccg atcgcgccag
    31561 catctgaatg acgtcggtct tactgcctgg gactgtgtga tcattaacca aatcattggt
    31621 tttattggct ttcaggcgcg gaccattgcg acatttcagg cttatctcgg gcacccggta
    31681 cgctggttac ccggtctgga aatacaaaac tacgccgacg cgtcactgtt tgctgatgaa
    31741 tcaatacgct ggcgaagcag ctatgaggtg gaaaaactac ctgaagagta cacaaaaagt
    31801 tcaacagcag aactttgcca actggccgaa acactctctc tccaccctat ttcactttcc
    31861 cttctcgaaa ggttgttaaa cagtacacgg gttaatacac agccggataa taagcttgcg
    31921 gcgttgttat gcgcgcggat aaatggcagt cctgcttgtt ttgccgcctg tatggattca
    31981 tcaaatgaat ataaaaaatc agcccccttc tgcgcaaggg cgaaaatgaa attaaccaat
    32041 gggctgaccg tcattctgtt gagcacgcta ccgttcaggc gatacaatgg ctgacccgag
    32101 cacccgatcg ctttagcgcc gcccagttca gccctttact cgaacacgaa aaatcatcaa
    32161 cgcagattat taatctgctg gtatggagcg ggctgtgtgg ctggataaat cgcttaaaaa
    32221 tcgcgttggg tgagacatat taaccttgcc gcgtcagaca gattcgcgta aaactgtcag
    32281 ccgctctaat ggccaccaaa atagacaatt atgtttcagg acaacccgct gctagcgcag
    32341 cttaaacagc aactgcattc ccagacgcca cgcgctgaag gggtggtaaa agccacagaa
    32401 aaaggctttg gcttcctgga agtcgacgcg caaaaaagtt atttcattcc gccgccgcag
    32461 atgaaaaaag tcatgcatgg cgaccgaatt atcgcggtga tccacagtga aaaagaacgt
    32521 gaatccgcag agccagaaga actggttgaa ccgttcctga ctcgtttcgt gggtaaagtt
    32581 cagggcaaaa atgaccgtct ggccatcgtt cctgatcatc cactcttaaa agacgccatt
    32641 ccttgccgcg cagcccgtgg cctgaaccac gagtttaaag aaggcgactg ggcggttgcc
    32701 gaaatgcgcc gtcatccgct gaaaggcgat cgttctttct atgctgaact gacacaatac
    32761 atcacttttg gtgacgacca ctttgtaccg tggtgggtta cccttgcgcg ccataatctg
    32821 gaaaaagaag caccagacgg cgtcgctacc gaaatgctcg atgaaggtct ggttcgtgaa
    32881 gatctgaccg cgctggattt tgtcaccatc gacagtgcca gcacagaaga tatggatgac
    32941 gcccttttcg ctaaggcgtt gccggatgac aaacttcagc tgattgtggc gattgccgat
    33001 ccaaccgcgt ggattgctga aggcagcaag ctggacaaag ccgcgaaaat tcgcgcattc
    33061 accaactatc tgcctggctt caacatccct atgcttcctc gcgagctttc tgacgatctc
    33121 tgctcactgc gcgccaatga agtccgcccg gtactggcat gccgcatgac gctctccgct
    33181 gatggcacca ttgaagataa tatcgaattc tttgccgcca ccatcgaatc caaagcgaag
    33241 ctggtgtatg accaggtttc tgactggctg gaaaataccg gtgactggca gcctgaaagt
    33301 gaagcaattg ccgaacaagt ccgtttgcta gcgcaaattt gccaacgccg cggcgagtgg
    33361 cgtcataacc acgcactggt gtttaaagat cgcccggatt accgctttat tctcggtgaa
    33421 aaaggtgaag tgctggatat cgtcgccgag cctcgtcgca ttgccaaccg tatcgtcgaa
    33481 gaagcgatga ttgccgctaa catttgtgcg gcccgcgttc tgcgcgataa gctcggtttt
    33541 ggcatctata acgtgcatat gggctttgat ccggcgaatg ccgacgcgct ggcagcgttg
    33601 ctgaaaacgc acggtctgca tgtcgatgcc gaagaagtgc tcacgctgga cggtttctgc
    33661 aaactgcgtc gtgaactgga tgcgcaacca actggtttcc tcgacagccg cattcgtcgc
    33721 ttccagtcat ttgctgaaat tagcactgaa cccggtcctc actttggcct cggtctggaa
    33781 gcatacgcca cctggacttc gccgatccgt aaatatggcg acatgatcaa ccaccgtctg
    33841 ctgaaagcgg ttatcaaagg cgaaactgcg acgcgtccgc aggatgaaat cactgtccaa
    33901 atggccgaac gtcgccgtct caaccggatg gcagaacgtg atgttggcga ctggttatac
    33961 gcacgcttcc tgaaagataa agccgggacc ggcacccgtt tcgcggcgga aattgtcgat
    34021 atcagccgtg gcggcatgcg tgtgcgtttg gttgataacg gcgctatcgc ctttattccg
    34081 gcacctttct tacacgctgt gcgcgatgaa ctggtttgca gccaggaaaa cggcactgta
    34141 caaattaaag gtgaaacggt ttacaaagta actgacgtta ttgacgtcac cattgccgaa
    34201 gtccgcatgg aaacccgcgg cattattgcg cgcccggtcg cgtaatctcc tttcacggcc
    34261 cattcctcat ggatgggccg tttatttccc cgctctacct tcgttatatc caggcagatt
    34321 ttttaactga ctttcgtttg aaaactggcg tttttccaaa actggattac aaataaatat
    34381 ataaaaaaac aatgcgttta gccgccgtta atttcatgta agtcatgttg ttccttttat
    34441 ttaaccgcgg actccgcagt taaccggagg atatgcatca tgaaaaccgt tagggagtcc
    34501 acaacgttgt acaactttct cggatcgcac aatccatact ggcggttgac ggaaagcagc
    34561 gatgttttgc gcttttctac caccgaaacc acagaacctg atcgtacttt gcagttatct
    34621 gccgaacagg ctgctcgcat cagggaaatg acggtcatca cctccagcct gatgatgagt
    34681 ctgaccgtcg atgagagcga tctttctgtg catctggtag gacgaaaaat caataaacgg
    34741 gaatgggctg gcaacgcgtc tgcatggcat gacacaccgg cagttgctcg tgatttatca
    34801 cacgggcttt cctttgctga gcaggtagtt tctgaagcac attccgcaat agtgattctc
    34861 gacagccggg ggaatatcca acgcttcaat cggttatgtg aagattacac tgggttgaaa
    34921 gaacacgacg tcattggcca aagcgtgttt aaactgttta tgagccgtcg tgaagctgcg
    34981 gcatccaggc gcaataaccg tgtatttttt cgaagcggca atgcatatga agtcgaactg
    35041 tggataccaa cacgtaaagg ccagcggctg tttctgtttc gcaataaaat aaatttgtcc
    35101 acagcggcag tggcaaaaac gagatttttt taatctgttc cggtaccgac attaccgaag
    35161 agcgccgcgc tcaggagcga ctgcgtattc tggcaaatac cgacagtatc accggactgc
    35221 cgaatcgtaa cgcaatgcag gagttaatcg atcacgctat taatcaggca gataacaata
    35281 aagttggggt tgtgtatctt gatttggata attttaaaaa ggtcaacgac gcctatgggc
    35341 atttgtttgg tgaccagtta ttacgcgacg tgtcattggc tattttaagc tgtctcgaac
    35401 atgaccaggt gttggcgcgt ccaggtggcg atgagtttct ggtactggca tccaatacct
    35461 cacaaagcgc gctggaagca atggcatcac gaattttgac ccgcttacgc ctcccctttc
    35521 gtattggttt aattgaagtt tataccagct gttcagtagg tatttcgctc tctcccgaac
    35581 atggttcgga cagcgcgact attattcgtc acgccgacac agcaatgtac acagcgaagg
    35641 aaggcggacg aggacaattt tgcgttttta ctccagaaat gaatcagcgg gtatttgaat
    35701 atctctggct ggataccaac ttgcgtaaag cactggaaaa cgatcagttg gttattcact
    35761 atcaaccgaa aatcacctgg cgtggcgaag tgcgcagtct ggaagcacta gtacgttggc
    35821 agtcacctga acgtgggttg attccaccgt tggacttcat ttcctacgcc gaagagtcag
    35881 ggctaattgt gcctttaggc cgttgggtga ttctcgatgt cgtacgccag gtggcaaagt
    35941 ggcgggataa aggcataaac ctgcgagtgg cggtaaatat ttctgcacgt cagctcgccg
    36001 atcaaaccat tttcaccgcc ctgaaacagg ttctccagga actcaatttt gaatactgcc
    36061 ctatagatgt tgaactgaca gagagttgtc tgattgagaa tgatgaactg gcactgtctg
    36121 ttattcaaca atttagccaa ctaggtgcgc aagtgcatct ggatgatttt ggtaccggct
    36181 actcttcact ttcgcaactg gcgcgctttc cgatcgatgc catcaaactt gaccaggttt
    36241 ttgttcgaga tattcacaaa caacctgtct cgcagtcact ggtccgggcg atcgtcgctg
    36301 tggcccaggc attgaatctt caggtgatcg ccgaaggtgt agagagtgca aaggaagatg
    36361 cttttttaac caagaacggg atcaatgagc ggcaaggatt tttgtttgcc aaaccgatgc
    36421 ccgccgtcgc cttcgaacgc tggtataaac gctatctgaa gcgcgcataa gtagatgaat
    36481 atatatactt agcgctatat cccgtcatta tatatcgtat tcctggatga taattagccg
    36541 ttgctgttaa acacttctct tttagcggca atttttgcat tattcatttt ccacttttgc
    36601 tataaggatg tccttgatgt ctgaatttga tgcccagcgc gttgccgaac gtattgatat
    36661 tgtgttagat atccttgtgg caggcgatta ccactctgcg atccataatc tcgagatact
    36721 taaagcagaa ctcttgcgcc aggttgctga atctacgcca gacatcccga aggcaccgtg
    36781 ggagatttaa caatcgtgcc ttcaggtttt cctatttgag gatgcccggc gcgtcagtat
    36841 ttttttattt agtattataa cgttataaga attacagcga tgaattcccg acaacaaact
    36901 attctacaga tggtcattga ccagggtcag gttagcgtaa ccgatctggc aaaagccact
    36961 ggagtttctg aagttaccat tcgccaggat ctcaacaccc tcgaaaaact gagttacctc
    37021 cgccgtgcac atggctttgc agtttcgctt gatagtgatg acgtcgaaac ccgtatgatg
    37081 agcaactata cgctgaagcg tgaactcgcc gagtttgccg cgtcactggt tcaaccgggc
    37141 gaaaccatct ttatcgaaaa tggcagcagc aatgccctgc tcgcccggac tctgggggag
    37201 cagaagaaaa atgtcactat catcacggtc agcagctaca tcgcgcattt gctgaaagac
    37261 gcgccttgtg aagtcatttt gctcggtggc gtgtaccaga aaaaaagcga aagtatggtt
    37321 ggcccgttga cacgccagtg catccaacag gtgcatttca gcaaagcatt tattggtatt
    37381 gatggctggc aacctgaaac tggattcacc ggtcgcgata tgatgcgtac cgatgtggtc
    37441 aatgccgtgc tggaaaaaga gtgcgaagcg atagtcctga ctgacagctc gaaatttggt
    37501 gctgtacatt catactccat cggtcccgtt gagcgattca atcgcgtgat taccgattcg
    37561 aaaatacgcg ccagcgatct gatgcatctt gagcacagta aactcaccgt tcacgtcgtt
    37621 gacatttaac catttccctt ctctacggat gatttgcagt ttggcaaatc atccgctcta
    37681 agatgattcc tggttgataa ttaagactat ttacctgtta ttaacacgct taagatataa
    37741 aattattatc agcgatataa caggaagtca ttatcacctg cgtgatataa ccctgcgcgc
    37801 gagcagattt cacggaataa tttcaccagg cttattctta gctattatag ttatagagag
    37861 cttacttccg tgaatcataa attcaggaga gagtattatg tttgtaacga gcaaaaaaat
    37921 gaccgcggct gttctggcaa ttactttggc aatgtctctg agtgcctgtt ctaactggtc
    37981 taaacgggac cgcaacaccg caatcggcgc gggtgcaggg gcattaggcg gtgcagtact
    38041 gaccgatggc agtacgttgg gtacattagg tggtgcagct gtcggtggtg ttattggtca
    38101 ccaggtcggt aaataatcgt aaataaatgt cgattatcga ctcgcaatta taatacgctt
    38161 gattctgttc agtacgaaat aactgaataa tacgcacagt aaaatattcg aaagccacgg
    38221 atatatccgt ggctttttat gttaaccgcc tgcgagtttt accttcatcc ctttcgcttc
    38281 aagtagtgat tttaataaat cacgcttatc gccctggatt tcaataactc catctttaac
    38341 tgctccgccg cagccgcatt ttttcttcag ttccgctgca agttttgtca gttcggcatc
    38401 atcgagatcg acaccggtaa tcaggcaaac gcccttacct ttacgtccac tggtctgacg
    38461 ctgaatacgc accacaccgt cgcctttagg gcgtacaggg gccgctttgg gctcatcaat
    38521 acgtccggtt tccgttgagt agaccagacg gctgttggaa tcactcatca tgcactccgc
    38581 tgtaaagagg cgttgatcgc tttcagcgtc tgcgctggat ctaccgattg cgttaccggg
    38641 cgaccaatca ccatataatc aacaccagcc gacaacgcct gttctggcgt cataatgcgg
    38701 cgctggtcac cagcttcact cccctgcgga cgaatgcccg gcgtaaccag tttgaactcc
    38761 tgaccgaata cctgtttaaa gcgcacagct tcctgagcag aacacaccac accatcaagg
    38821 ccacattttt gcgtcagtgc cgccagacgt tctgcataat ctgcaggtga cagtgtcatg
    38881 ccaagatcga ccaggtcgct ggcttccatg ctggtcaaca ctgtcacagc aatcaaaagc
    38941 ggtgcatctt tgccaaacgg aaccagtgcc tcacgcgctg cggtcatcat acgcgcccca
    39001 ccagaggcat gaacattcac catccacacg cctaagtcag ctgcagcagc gacagcgtgc
    39061 gctgcagtgt taggaatatc gtggaatttc aggtcaagaa agatatcaaa accacgctgt
    39121 tgaagttcgc gcacaaactg tggcccaaac aatgtaaaca tctctttgcc gaccttcaga
    39181 cgacaatcgc gtgggtcgat cttgtcgaca aaggacagcg cgtcatcacg attatgataa
    39241 tcaagggcaa caaccacagg agaattcgta acagcgcggg aagaagatga agcagttaac
    39301 gtcatgacca gaccttcttg atgatgggca ccggaagtgg cgcgaaacag gtaaacggcg
    39361 agcattctac ccgtggaaat aaaaaattaa cagttgcgat ttttcccctg gcaggctttt
    39421 tctgctccgt cgttgccgga gcacaatgga acatcaataa ctagtatgtt gtaactaaag
    39481 tcggattttt ttaaaaatta caggccatca agaccgcgaa tcggtttaat ggttgaccag
    39541 gcccgacaag acggacaatg ccagtagagg gtgtatgcgg taaaaccaca tttctggcag
    39601 cgataacgag gcttactacg taccttttcg ccaaccatgt cacgcagcac catcaggctc
    39661 tctttggcac gcccttcttc cgcttcattt aagtggtaat ccattaactt atggaacaca
    39721 cgcatggtcg gatgacgctg aagctggcgc gtaatataga cctgtgcggc ctcactaccg
    39781 tcgcgcgctt cgatgatatc agcaagcatc aattcagcat cggcaccggt gttctcttcc
    39841 accgcgcgtt gcaggaattc tgcccattcg gcagttttac ccaactgctg atagcaggtt
    39901 tgcaacattt ccagcgtttc gctgaccagt tctctgtcct gggatatcac gcgttgcaaa
    39961 ctttcgacgg ctttggcgta ttctcctttc gccataaaca cgcgtcccat catgatggat
    40021 acgcgggcgc tgtttttatc tgccgccgcc ccttttttta gcaaggtcat ggcacgatcg
    40081 agatcgtcgc tggccatatg ctgcagggct aactcacagt agaaatgggc aatttcgacg
    40141 cgctgtttat ctttacccag cttcaccagg cgttcggcaa catcaattgc tttctgccac
    40201 tcgctggtag cctggtagat ttgtagcaac tgttgcagcg cgccaatgcg gaagtcagtt
    40261 tcatcggtca gctgattgaa catgtcttcc gcgcggtcgt ataacccggc ggccatgtaa
    40321 tcacgcccca gttgttgaat cgccaacaga cgctgttcat aggtcagtga ggcgctttcc
    40381 attagggtct gatggatgcg aatagcgcga tcaacttcgc cacgcgaacg gaacaggttt
    40441 ccgagcgtaa ggtgggcttc aacggtgcct gtatcctctt taagcatatc gagaaacagg
    40501 tctaccgctt tatcctgttg attactaagc aggaagttaa cccccgctac gtaatcacgc
    40561 gacaagcggt tggcttcatc ttgcttgttt tgttgcgcac ttctgcggcc catataccag
    40621 ccataggcag cggctacagg caaaagcaga aacaacaact ccagcataga aagttattcc
    40681 ttcgccgctg acgagtgcgg cactacagcc acgtcagtcg cgggtgaaag ctggttttcc
    40741 agtcgcttta ttttacgttc agcgcgcgcc agggaaacac gaactcgcag ccagaacagg
    40801 ccacaaatca accaaccgat agcaaccccc gcagcaaaca ataccgccag caatgtggaa
    40861 atacggtact ccccttgcgc taacagataa ttaaacgtca cctgttgatc gttctgcgca
    40921 cccaacgtga ccgaaatcac gaagatcgct aacaccagta agaaaatgag taaatatttc
    40981 acattacttc ccgttatgtg gttaaagcga ataaattatg gtcaacttac cgcaatagcc
    41041 ctttaaagta ccattttcgc gccgtctgcg aaatggcatc cacgcgcatt gttgatgatt
    41101 taagggctaa attcggaaaa tcaaccagcg ttaactttct tgttctcgtt gcgctatttc
    41161 gcgattttct tccgcaggtg gtgttaatgg cccacaaatt cgttgcgcaa gccacgttgc
    41221 caccgccacc agcgcccacg aaatcaacgt agctactacc agatcgcgtg gccaatgcat
    41281 cccgagcagc aggcggcttc ccatgactcc cgttgcccag accagcaaga tagcaatggt
    41341 taacgttcgc cgacgcggcc acagcaaacc aacggccagc agtgcccaac tggcagcaaa
    41401 catcgtgtga ccggaaggaa aggcaaaccc cgtctctttc tgccagtgtg aacgcaaata
    41461 ttgtgggata tttttctctt cagccaactg ttctttcact agatttccgc gttctgctcg
    41521 ctttaaagtg tagaactcat caaccggaat atgatgtgtt ttttccagcc agataacaaa
    41581 aggtcgtggt tcctggactt tgtctttgat ccaggattta acgccttgtc ccacaatgat
    41641 tacggccgcc agaatggcaa ataatacaaa ggcagcctta atgcgaaaac gcagacacca
    41701 gagaaaccag ccgaataaaa tcaaatgtgt aatgacgccc cagggctggg tgacagtttc
    41761 agtaacccaa aaagccgctt ttagtagcca actttgttct ccaggttgcc aacgccagcc
    41821 agaaatccat acggctactg gcatgacaag caatagtgca gctcccactg cggtacgtct
    41881 ggcaatcgaa cgcatggcct ctcctttttg ataagtccca caatcataac tgaaaacgcc
    41941 agttccagga aaaattgaca gatttgtgcc attccgtgaa cgatcgacgc gtcgtgatta
    42001 ggtgaacccc ttctcgttat ggcaaaataa gccaatacag aaccagcatt atctggagaa
    42061 tttcatgcag cttaaacgtg tggcagaagc caaactgcca accccatggg gcgatttcct
    42121 gatggtggga tttgaagaac tggcaaccgg acacgatcat gtcgcgctag tctatggcga
    42181 tatttccggg cataccccgg tacttgcgcg cgtccattcc gaatgtctga ccggtgacgc
    42241 cctgttcagc ttgcgctgcg attgtggctt ccagctcgaa gcggcattga cgcaaattgc
    42301 cgaggaaggc cgtggtattt tgctgtatca ccgtcaggaa ggtcgtaaca ttggtctgct
    42361 gaataaaatc cgcgcttacg cactgcagga tcaaggttac gataccgtag aggctaacca
    42421 tcagttaggc ttcgccgctg atgagcgcga cttcactctt tgcgctgata tgttcaaact
    42481 ccttggcgtc aatgaagtcc gcttgttaac caataacccg aaaaaagtcg aaattctgac
    42541 cgaagcaggg attaatattg ttgaacgcgt accattgatt gtaggtcgta accccaataa
    42601 cgaacattat ctcaatacca aagccgagaa aatgggtcat ttgctgaaca aataaccctc
    42661 tcgcattgtg gaattaattt gcttgccgga agcaaaataa ccggcaagca aatatttgtt
    42721 acttcagcat attacgaatg acataatgca aaatgccgtc gttctggtag taggtcaact
    42781 ccgtcgcggt gtcgatacga caacggcagg gtacgacctc ctggctacca tccgcgcgcg
    42841 taagcgtcac cggaaccgtc gcaccgggtt gtagattttg cagatcgcca atatcaatct
    42901 tctcttcccc ggttagtcct aacgttttac gcgttacgcc ttgcggaaat tccagcggca
    42961 ggatgcccat gccaattaaa ttcgaacggt gaattcgttc aaacgattcg gcaatcacca
    43021 cacgaattcc aagcagacga ggaccttttg ccgcccagtc acggcttgag cctgaaccat
    43081 actctttccc ggcaatcacc gccagcggcg tttgttcctg cttatagcgc atcgcagcat
    43141 cataaataga gactacgtcg ctgtcaggta aatgccgcgt catcccccct tcaacgccag
    43201 gcaccatttc attacggatg cgaatattgg cgaaggtgcc gcgcatcatc acttcatggt
    43261 taccacgccg cgaaccgtag gagttaaagt cttttcgctc aacaccccga ccttgtagat
    43321 atcgacccgc tgggctgtcg ggcttaatac tgcccgccgg agagatatgg tcagtggtga
    43381 ctgaatcccc cagcattgcg aggatccgcg caccgtgaat atcttccact ggtgctggtg
    43441 ttgcctgcat ttcatcaaag aaaggcgata agcgaatata ggttgagtcc tcctgccaac
    43501 cgtaggtatc ggatcgtgtg acgttaattt ccttccactc tgctgtgcct tcaaaaactt
    43561 ctgcgtactc tttgcggaac atttctgtgg agacttgttc taccgcacgg gcaatttctt
    43621 gtgccgatgg ccagatatct ttcagataaa ccggatcgcc tttacgatca tgaccaatag
    43681 gctcagaagc caggttgata ttcatatttc ccgccagcgc ataggcaacc accagcggcg
    43741 gcgaggccag ccagttggtt ttaaccagcg gatggatacg gccttcaaag ttacggttgc
    43801 cggacagcac tgcaccgacg gttaaatcgc cttttttgat tgccgtttcg ataggatcgg
    43861 gcagcggccc agagttacca atacaggtag tacaaccgta tcccacaagg ttaaacccca
    43921 gttcgtcgag aaacggtgtc agtttcgctt ttgccagata gtcagaaaca actttcgagc
    43981 ctggtgccag cgacgctttg acccacggtt gccgcttgag gccaagaatt acggcttttt
    44041 tcgccagcaa gcctgcagcc atcagcacac ttgggttaga ggtgttggtg cacgaggtta
    44101 tcgcagcaat gaccacagcg ccatcaggta actgatactg atgtccgttc ataacataat
    44161 cgaccggctg gcgatcttta tgcgtggcat tcacttccag ttcgttactg gcggcaaata
    44221 cttttggtac atcgggcagt gcaacgcgat cctgtgggcg ttttggccct gccaggctcg
    44281 cttcaacgtc attcatatcc agttctaacg tactggtaaa aatcggttca tcgcccgggt
    44341 tacgccacat gccctgcgct ttggcatatt tttcgaccaa ctcaacctga tcttcgctgc
    44401 gcccgcttaa gcgcatgtaa tcgagggtta cagcatcgat tgggaagaag ccacaggtgg
    44461 caccatattc tggcgacata ttggcaatgg tggcgcgatc cgccagcggt agtgaatcca
    44521 gaccatcacc ataaaattcg acgaatttct ccaccacgcc atgtttgcgc agcatttggg
    44581 taacagtgag aaccaggtct gtggcggtaa taccttcacg taatttaccg gtaagtttaa
    44641 agcccactac gtccgggata agcatggaaa ccggctggcc taacattgcg gcttctgctt
    44701 cgatcccacc cacgccccac cccagcacgc caaggccgtt gatcatagtg gtgtgcgagt
    44761 cagtaccaac gagtgtatcc ggataagcaa tccattcacc gtcctgcaat tcactccaca
    44821 ctgctttgcc gagatattcg aggttaacct gatggcaaat gcctgtgcct ggcggcacga
    44881 cgctaaaccg actgaacgct tgctttcccc atttcaggaa cacataacgt tcgtggttgc
    44941 gctccatttc caggcgtacg ttttcttcaa atgcctcatc atcaccaaaa cgatcgacgg
    45001 tcaccgagtg gtcaatgacc aggtcgaccg gtgagagcgg gttaaccttt gcagtatcgc
    45061 cgccgaggcg tttaaccgct tcgcgcattg ccgccagatc aacaacggca ggtacgccgg
    45121 taaagtcctg catcaacacc cttgccgggc ggtaggcaat ttcacggtca gcatgggcat
    45181 ttttcagcca tcctgccagc gcgtggatat cctcttcggt aaccgagtta ccatcctgcc
    45241 agcgcagcag gttttcgagc aaaactttga gtgacttggg tagacgggtg atatcgccca
    45301 gtgatttagc agcaagcggc aggctgtagt agtggtaagt tttatctttg gcctgcaacg
    45361 tgtccttact ggcttctcgt agggttgacg acatagctcc tccttaatga cagggttgcg
    45421 aagccctgat tctcttcagg gtgaatatta aagataacac aaacatcgcg taacgatttg
    45481 ataacaaccc aaattgataa aagaggagaa accttgtggc aaagcaaatg acaaccccgc
    45541 cgcagcgggg tcaaggatca gtgagtaatg atccagagga gttcggccca gaagagcatc
    45601 gacagagaaa acgcgcccat ccaggaccaa tatttcatat ttgtgttgat cataggctac
    45661 acccttaacg gttattattt tgtgttgaga taactcaagc tgttattgct gcggtgtatt
    45721 aaaagccctt taattcatta aaaatgggta ataagaatga tgataattat tgtttcggct
    45781 gatcatcttc agcaaacagt tctataaagg cttgctgctg cgatgttagt tgccagtcgt
    45841 ctggaataac gacggaacat tcccgtttcg tttctgtttc attgtcacta tgctggcata
    45901 atggtgacga ctccagttgc tcctgaccga ccatagttat ccccacactt tccaaatcag
    45961 cagtgcgaca accacccaga aaagtgcaga accaagaaaa accgccagcc aggc
//
LOCUS       DAZPLI010000017        44203 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZPLI010000017 DAZPLI010000000
VERSION     DAZPLI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44203
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.017"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..559)
                     /locus_tag="ACHUU3_000891"
     CDS             complement(<1..559)
                     /locus_tag="ACHUU3_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR2081046.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(762..998)
                     /gene="feoC"
                     /locus_tag="ACHUU3_000892"
     CDS             complement(762..998)
                     /gene="feoC"
                     /locus_tag="ACHUU3_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR2081047.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(998..3319)
                     /gene="feoB"
                     /locus_tag="ACHUU3_000893"
     CDS             complement(998..3319)
                     /gene="feoB"
                     /locus_tag="ACHUU3_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR2081048.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="ACHUU3_000894"
     CDS             complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="ACHUU3_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR2081049.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4001..6322)
                     /locus_tag="ACHUU3_000895"
     CDS             complement(4001..6322)
                     /locus_tag="ACHUU3_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR2081050.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6419..6895)
                     /gene="greB"
                     /locus_tag="ACHUU3_000896"
     CDS             complement(6419..6895)
                     /gene="greB"
                     /locus_tag="ACHUU3_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR2081051.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7123..7842
                     /gene="ompR"
                     /locus_tag="ACHUU3_000897"
     CDS             7123..7842
                     /gene="ompR"
                     /locus_tag="ACHUU3_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR2081052.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7839..9191
                     /gene="envZ"
                     /locus_tag="ACHUU3_000898"
     CDS             7839..9191
                     /gene="envZ"
                     /locus_tag="ACHUU3_000898"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR2081053.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="ACHUU3_000899"
     CDS             complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="ACHUU3_000899"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR2081054.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKLDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11268..12968
                     /locus_tag="ACHUU3_000900"
                     /pseudo
     CDS             11268..12968
                     /locus_tag="ACHUU3_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="ACHUU3_000901"
     CDS             complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="ACHUU3_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR2081055.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="ACHUU3_000902"
     CDS             complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="ACHUU3_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR2081056.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="ACHUU3_000903"
     CDS             complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="ACHUU3_000903"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR2081057.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="ACHUU3_000904"
     CDS             complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="ACHUU3_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR2081058.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17606..18166
                     /gene="nudE"
                     /locus_tag="ACHUU3_000905"
     CDS             17606..18166
                     /gene="nudE"
                     /locus_tag="ACHUU3_000905"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR2081059.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="ACHUU3_000906"
     CDS             complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="ACHUU3_000906"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR2081060.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21003..21782
                     /gene="hofM"
                     /locus_tag="ACHUU3_000907"
     CDS             21003..21782
                     /gene="hofM"
                     /locus_tag="ACHUU3_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR2081061.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21782..22321
                     /gene="hofN"
                     /locus_tag="ACHUU3_000908"
     CDS             21782..22321
                     /gene="hofN"
                     /locus_tag="ACHUU3_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFR2081062.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTD
                     NAEARVNTVLLQAEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            22305..22745
                     /gene="hofO"
                     /locus_tag="ACHUU3_000909"
     CDS             22305..22745
                     /gene="hofO"
                     /locus_tag="ACHUU3_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR2081063.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22735..23139
                     /gene="hofP"
                     /locus_tag="ACHUU3_000910"
     CDS             22735..23139
                     /gene="hofP"
                     /locus_tag="ACHUU3_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFR2081064.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23051..24289
                     /gene="hofQ"
                     /locus_tag="ACHUU3_000911"
     CDS             23051..24289
                     /gene="hofQ"
                     /locus_tag="ACHUU3_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR2081065.1"
                     /translation="MKQWIAALLLMLIPAVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24690..25211
                     /gene="aroK"
                     /locus_tag="ACHUU3_000912"
     CDS             24690..25211
                     /gene="aroK"
                     /locus_tag="ACHUU3_000912"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR2081066.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25268..26356
                     /gene="aroB"
                     /locus_tag="ACHUU3_000913"
     CDS             25268..26356
                     /gene="aroB"
                     /locus_tag="ACHUU3_000913"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR2081067.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26448..27734
                     /gene="damX"
                     /locus_tag="ACHUU3_000914"
     CDS             26448..27734
                     /gene="damX"
                     /locus_tag="ACHUU3_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR2081068.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27841..28677
                     /gene="dam"
                     /locus_tag="ACHUU3_000915"
     CDS             27841..28677
                     /gene="dam"
                     /locus_tag="ACHUU3_000915"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR2081069.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28695..29372
                     /gene="rpe"
                     /locus_tag="ACHUU3_000916"
     CDS             28695..29372
                     /gene="rpe"
                     /locus_tag="ACHUU3_000916"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR2081070.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29365..30123
                     /gene="gph"
                     /locus_tag="ACHUU3_000917"
     CDS             29365..30123
                     /gene="gph"
                     /locus_tag="ACHUU3_000917"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR2081071.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30116..31120
                     /gene="trpS"
                     /locus_tag="ACHUU3_000918"
     CDS             30116..31120
                     /gene="trpS"
                     /locus_tag="ACHUU3_000918"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR2081072.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31280..32185
                     /gene="yhfZ"
                     /locus_tag="ACHUU3_000919"
     CDS             31280..32185
                     /gene="yhfZ"
                     /locus_tag="ACHUU3_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR2081073.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32202..32564
                     /locus_tag="ACHUU3_000920"
     CDS             32202..32564
                     /locus_tag="ACHUU3_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR2081074.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32648..33811
                     /locus_tag="ACHUU3_000921"
                     /pseudo
     CDS             32648..33811
                     /locus_tag="ACHUU3_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33811..35037
                     /locus_tag="ACHUU3_000922"
                     /pseudo
     CDS             33811..35037
                     /locus_tag="ACHUU3_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            35034..35912
                     /locus_tag="ACHUU3_000923"
     CDS             35034..35912
                     /locus_tag="ACHUU3_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR2081075.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35923..36276
                     /locus_tag="ACHUU3_000924"
     CDS             35923..36276
                     /locus_tag="ACHUU3_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR2081076.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36288..37592
                     /locus_tag="ACHUU3_000925"
     CDS             36288..37592
                     /locus_tag="ACHUU3_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR2081077.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37604..38689
                     /locus_tag="ACHUU3_000926"
     CDS             37604..38689
                     /locus_tag="ACHUU3_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR2081078.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38841..39572)
                     /gene="frlR"
                     /locus_tag="ACHUU3_000927"
                     /gene_synonym="yhfR"
     CDS             complement(38841..39572)
                     /gene="frlR"
                     /locus_tag="ACHUU3_000927"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR2081079.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="ACHUU3_000928"
     CDS             complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="ACHUU3_000928"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR2081080.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALCLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="ACHUU3_000929"
                     /pseudo
     CDS             complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="ACHUU3_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="ACHUU3_000930"
     CDS             complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="ACHUU3_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR2081081.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42378..43715)
                     /gene="frlA"
                     /locus_tag="ACHUU3_000931"
     CDS             complement(42378..43715)
                     /gene="frlA"
                     /locus_tag="ACHUU3_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFR2081082.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            complement(44010..44177)
                     /locus_tag="ACHUU3_000932"
     CDS             complement(44010..44177)
                     /locus_tag="ACHUU3_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR2081083.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT        10313 a        11213 c        12098 g        10579 t
ORIGIN      
        1 cgcgttgacg aatatgtttt tgaattaact ccagcagcgc catacgtcgg tgctgcatga
       61 tttcatcatc aggcatgacg gtgacatcaa tcagcggaaa tgcggaggca taaagctgcc
      121 ttgccagttt gggatcggcg aaacaatcca gccaacacag cgaataggga taggggcttt
      181 caataccgtg gtaaaacaac aatagcacca ccatcggcaa cgttttgtat ccagcatcaa
      241 ggtgattttg cattgcggca atagcgtaac gcatcatgcg aaatgcgatc agtttgtttg
      301 aggtgctttg atgttcaatc agacaataga tgtatcctgg tccttgttcc gttttcaccg
      361 accacagcac atcggaatag ctttcacgca gatcgtcatc aataaagctg ctcgactcca
      421 gcttgagggt tttcatatca caaagcgcgt gaatgggtgc cggtaaatga aacgcaagaa
      481 aatcacgagc cgtgtccggt tggcgtaaaa agagtttgaa cagcgcatcg tgtggggtgg
      541 aactctgctt tttgctcatg gcgttccttc acctgttgtt tgaatgccgc gactctacgc
      601 gccactccac acctgtgcat tagcgagttt tcatccctgc gagatgcttt caaaggaact
      661 tacgccgtgc tgcaagagtt gcatttttat ggaaagccgc acgcaaaaaa gcccggatgc
      721 agacgcatac cgggctttcc aaaacaggcg atggagtaag gttaacgcag cgcccaccac
      781 tcgcgcagac aggcttttcc ttccgggcag cttttacaac tgccagagag gcagccgtca
      841 ggttcttcct gaatccgcac ggctttgccc atactttcca gttgttgcag catggcgtta
      901 atcattggct gcggagtgtt caatgtctgg cttatctggg tcgcttccat acggccccgt
      961 aacgccagca aatcacgtac ctgaataagt gaagccatta atggcaatca ccggtggtgc
     1021 tggctgcgca gcaactgctt accgacttgc gggttgccag cagttcgata tccacccggc
     1081 tacgcgcgcg gcgcagcaga ccgataacta cgatgttaaa caggattacc gccagaatgc
     1141 acaccaggct gtaagttggg tgctgactgt agctggcgac ctgatagaac aatgttgcca
     1201 gtgagtaagc gatattcagc ccccacagga tggagaagcc catccagcca cggcttgatt
     1261 cacgggcgat ggctcccatc accgagatac atggtacata cagcaggacg aaaatcaggt
     1321 agctgtatgc ggcagctgcg ctaccgaatt tctgatccat cacgcccatc gccccggtac
     1381 ccatttcacc gtcgcctttg ctagcttcaa tggggttcat cagtacgcta aggctgaagg
     1441 tgtctttcag gctctgccag gtttcatcta ccgcactgaa cagctcttca ccgaggttga
     1501 attccgccgg attgaactct tcgtcctgaa tattttctgc ggtgtagagg gtgttgagcg
     1561 tacccaccac cacttctttt gccattgcgc ccgtaaacag gccaaccgtt gcctgccagt
     1621 tatcttcatg tacgccaatc ggcttgaaga caggggttat cacccggctg acggacgcca
     1681 gcgccgagtc gttgatgtta tcgacgattt tcccgctcaa cgagaagctg ttgaaagcgc
     1741 tcaggaaaat gctgacgatg ataatcactt taccagcacg cagaacgaag cccttcagac
     1801 gctgccaggt ctggataatc aggcttttaa cgtgtggtac atggtagacc ggcagctcca
     1861 tgacaaacgg cgtcgcttca ccgcgcatga tggtgtactt gagcatcagg ccagtcagca
     1921 ccgccatcac gataccgagc atatacagcg agaaaaccgc cagggcaccg ttctgcccga
     1981 agaaggcagc cgcgaatact gcgaagatag ccagacgcgc gccgcaggac ataaacggtg
     2041 ccatcatgat ggtcatcaga cgttcacgcg gtgcatcaag cgtacgtgcg cccattaccg
     2101 acggtacgtt acaaccgaaa ccgacgatca gcggcacaaa ggatttcccc ggcaagccca
     2161 gcgcctgcat caggcggtcc atcacaaacg ctgcacgcgc catatagccg gagtcctcaa
     2221 ggaaggagag gaacaggtac atcatgccga tctgcggcac cagtggcagc acggtgttaa
     2281 taccgccacc caggccctgg gcgaggaaga tagtcagcca gtccgggaag tggagcgtgt
     2341 agccaatcca ttgaatacca tgcacaaata gcgccacgga gccgacgtca aacagcggct
     2401 gtaacgcccc gccgatgttg atagccagca ggaacatcag gtacatcaca aagaggaaaa
     2461 tcggcagacc gaggaaacgg ttgagcacga ttttatctac cgcagtggtg aaacggctgg
     2521 gttctgccgt cagggtgttg cttaccacat cacagatggc agctatgcac tggtaacgcg
     2581 catcggcaat gtgcagcgcc ggatcatcca tttcattacg cagacgggca agggcggcat
     2641 ccagatgctg cgacgcttca ccggcgtagg cgcggctgta gatatcgcct tccagcattt
     2701 gcaggcccag ccagcgacgt tgtttcagcg ggatgtcgga aggcatcact tttgccagtg
     2761 aatctgcttc gttgagtagc ggctgtgcgt aatgcaccag ttccacattc tcgttagctt
     2821 tatagcgatc aatcgccagc ttgagcgctt caataccgcg accacgggtt gaaaccagcg
     2881 ggatcactgg gcagccaaga cgcgccgaca gagcatcaat ttcaatacga atattttgct
     2941 tctcggcaat gtcgagcatg ttcagtgcca cgatgcaggg aatgccgagt tccagcagtt
     3001 gtagcgtcag gtacaggtta cgctcaaggt tagacgcatc caccacgtta atcagcaggt
     3061 cggcgtcgcc actcaaaatg tagtgacagg cgatttgctc atcgagcgag gtctgcgatg
     3121 agatggtggt cagagaatag gtgccgggca ggtccaccag cgtgacctga tgatcggtgg
     3181 tggagaattg cccttcttta cgttcgacgg taacgccagc ccagttacct acacgctgac
     3241 gtgcgctagt gagctggtta aataaggttg tcttgccaga atttggatta ccaattaagc
     3301 caatggttaa ttttttcatt gttgttatca ccgtattaac aggaaaccgc ttccacttct
     3361 aataaggcca gatctttttt gcgtaatacc aggctcacac gacgggtttc gatatgaatg
     3421 gggtcgccga gtggagcgac gcgcaccaca ttaaaagagg agccaggtaa catgccaaga
     3481 gaaagcagtt tttggcgata tgccgggctg atttcacggg aaaagccagt gattttccac
     3541 gcagtatctg gagtgtattg cataggtgcc tacttgtttc tcattaactg gataactacc
     3601 tcatggggcg tttgacagcg acatgagacc agatgatttg ccaacaaagg agagattaaa
     3661 taatcaacaa cacaatgata atgagaatgg tttctatcag caatacttaa aatgtgtgcg
     3721 aatgttggtt tgaataataa tctgactcaa tgttgatgtg gctcaaggtt atatcgcagc
     3781 gaaaatataa ttcgaatagt gaattacccg gactggctaa tgtttaatta aggtttcagt
     3841 tgttaatgaa gtgataaata ttgccggaat atattatgag tttggttgtg gaataaaaag
     3901 gccggagata ttctccggca cttttatgtc aggcaaggcg ttcgttgcca gatgcggcgt
     3961 gaacgcctta tcgcgcaggt ttaggggcca gtccgggcgt ttaacgtttt ttgcccattg
     4021 ccgccgccag cgcatccatc atcgcgctat taccggcagg ctgcgcttca cgaccgcgtg
     4081 gtttggctgc cgggcggttg ttttgcgggc gttcattacc gccgccgcga cgggcgttgg
     4141 tttcgccagg ctgctcgtcc agacgcatgg tcagggcgat acgtttacgc tgaagatcca
     4201 cttccagcac tttcaccttc acaatgtcgc ccgctttcac cacggtatgc ggatcttcca
     4261 caaacttgtt cgacaatgaa gagatgtgaa ccaggccgtc ctgatgcacg ccaatatcga
     4321 caaacgcgcc aaagttggtg acgttggtga cagcgccttc gaggatcata cccggttgca
     4381 ggtcgttcat tgtctcgacg ccatcggcaa actgagcggt tttaaattcc ggacgcggat
     4441 cgcgacccgg tttttccagc tctttgatga tgtcagttac tgtcggcaca ccgaattttt
     4501 catcagtaaa gtcagacgct ttcaggttac gcagttcgct gctgttaccc atcagatctt
     4561 tcagtgcctg ctgtgttgct gccagaatgc gttccaccac cggataggct tccgggtgaa
     4621 cggtagaggc gtccagcggg ttatcaccgt ggttaatgcg caggaagccc gcgcactgct
     4681 cgaaggcttt cggccccaga cggctgactt tcagcagttg ctgacggttc ttgaactggc
     4741 cgttctcatc gcgccaggcc acgatgtttt gcgccatcat gcgcgtcagg cccgccacgc
     4801 gagttaatag cggaacagaa gcggtgttga gatcgacgcc aacggcgttt acgcagtctt
     4861 ctactactgc atccagtttg cgggccagtt gcgtctggct gacgtcatgc tgatactgac
     4921 ctacgccgat agatttcgga tcgattttca ccagctccgc cagcggatcc tgcaaacggc
     4981 gggcgataga taccgcacca cgcagcgaaa cgtcgagatc cgggaactcc tgcgctgcca
     5041 gctcggaagc tgagtaaacc gacgcgccag cttcgctgac gatcactttc tgtgcggtca
     5101 ctttcgggaa ctgcttctgc acgtcgagat agaaacgttc agtttcgcgg gaagctgtac
     5161 cgttgccgat cgccaccagt tcaacgttat gtttttcaca caaggcagca acggtcatcg
     5221 ctacttttgc ggcttgtccg gtgtgcgggt aaatggtatc ggtcgccacc agtttgccag
     5281 tggcatcgac cactgccact tttaccccgg tacgcagacc cggatcgagg cccatcgttg
     5341 cacgcagtcc ggcaggggcc gccatcagca gatcgtgcag gttacgggca aagacgttga
     5401 ttgcttcatc ttccgcgcgt tcgcgcacgg tacccatcag ttcggtttcc agatgcatca
     5461 gcaccttgat gcgccaggtc cagctcacta cgcctttgcg ccagctatcc gccggggcat
     5521 tgttcaggcg caggccaagg tgatccatga tgatttgctc gcaatagctc tctttgggcg
     5581 gctcatcgaa ctgcggatcg gcattcagcg aaagctggag tacgccttcg ttacgcccac
     5641 ggaacatcgc cagcgcgcgg tgagaaggca ccgtggacaa cggttcgtga tgatcgaaat
     5701 agtcgcggaa tttcgcccct tcctcttctt taccgctcac caccgtagaa accaaatgcg
     5761 cgttcttcca cagataatca cgcactttcg ccagcagcgc ggcatcttcg gcaaaccgtt
     5821 ccatcaggat atagcgcgcg ccgtccagcg cggctttggt atctgccacg cctttatcgg
     5881 catcaatata ttgcgcagcg gcgacttctg gtgtgtgtga cggatcgctc cacagcaggt
     5941 cagccaacgg ctcaagccct gcttcaatgg cgatttgccc gcgggtgcgg cgtttaggtt
     6001 tgtagggcag gtagaggtct tcgagttcgg ttttgcttag ggtggcgttg atggccttcg
     6061 ccagatcatc ggtgagtttg ccttgctcgg aaatggactt gaggatcgcc tgacgtctct
     6121 cttccagctc gcgcagatag ctcagacgcg tttccagatt acgcagctgc gtgtcatcca
     6181 gaccgccggt gatttcctta cgataacgtg cgataaacgg cacggtattc ccttcgtcaa
     6241 gcaggcgaac ggcagcgtca acctgttccg ggcgcgcctg aatttcaccc gcaataatgc
     6301 ggcagaacga atcattcatc atggtttgag ttcatctttt cggatcaaaa atcaggggat
     6361 agttatacgg actggctggc aaaaatgcca gccatcggca ggaggttaag actcttcatt
     6421 acggtttcac gtattcgata gcattaacat accagctcgc ttccccggca ggggtattca
     6481 ccaccgccag atcgccgact tcttttttca gcaatgcgcg ggccatcggg gaatcgatag
     6541 agatgtaatc tttacggcca aaaatttcat cgtagccgac aatacggaaa cggtgagtca
     6601 cgccatcgtc gttttcaatc tccacccacg cgccaaaaaa gactttgcct tcctgctgag
     6661 gggagtaatt gacgattttg agattttcca tgcatttagt gagatagcgc acgcgacggt
     6721 cgatttcacg cagacgcttt ttattatact gatagtcagc attttcgctg cggtcgccca
     6781 gacttgcggc ccaggtcacc ttttttgtga cctccgggcg ttcttcacgc cagagataat
     6841 taagctcttg tttgagtttt tcataccctt cccgggtaac caggggcgtt ttcatctcgt
     6901 tgattccctt tgtctgtttg ataatgcgca cattgggtat aacgtgatca tatcaacaga
     6961 atcaataatg tttcgccgaa taaattgtat acttaagctg ctgtttaata tgctttgtaa
     7021 caatttaggc tgaaattcat accagattta gctggtgacg aacgtgagct tttttaagaa
     7081 tacacgctta caaattgttg cgaacctttg ggagtacaaa caatgcaaga gaactacaag
     7141 attctggtgg tcgatgacga catgcgcctg cgtgcgctgc tggaacgtta tctcaccgaa
     7201 caaggcttcc aggttcgaag cgtcgctaat gcagaacaga tggatcgcct gctgactcgt
     7261 gaatctttcc atcttatggt actggattta atgttacctg gtgaagatgg cttgtcgatt
     7321 tgccgacgtc ttcgtagtca gagcaacccg atgccgatca ttatggtgac ggcgaaaggg
     7381 gaagaagtgg accgtatcgt aggcctggag attggcgctg acgactacat tccaaaaccg
     7441 tttaacccgc gtgaactgct ggcccgtatt cgtgcggtgc tgcgtcgtca ggcgaacgaa
     7501 ctgccaggcg caccgtcaca ggaagaggcg gtaattgctt tcggtaagtt caaacttaac
     7561 ctcggtacgc gcgaaatgtt ccgcgaagat gagccgatgc cgctcaccag cggtgagttt
     7621 gcggtactga aggcactggt cagccatccg cgtgagccgc tctcccgcga taagctgatg
     7681 aaccttgccc gtggtcgtga atattccgca atggaacgct ccatcgacgt gcagatttcg
     7741 cgtctgcgcc gcatggtaga agaagatcca gcgcatccgc gttacattca gaccgtttgg
     7801 ggcctgggct acgtctttgt accggacggc tctaaagcat gaggcgattg cgcttctcgc
     7861 cacgaagttc atttgcccgt acgttattgc tcatcgtcac cttgctgttc gccagcctgg
     7921 tgacgactta tctggtggtg ctgaacttcg cgattttgcc gagcctccag cagtttaata
     7981 aagtcctcgc gtacgaagtg cgtatgttga tgaccgacaa actgcaactg gaggacggca
     8041 cgcagttggt tgtgcctccc gctttccgtc gggagatcta ccgtgagctg gggatctctc
     8101 tctactccaa cgaggctgcc gaagaggcag gtctgcgttg ggcgcaacac tatgaattct
     8161 taagccatca gatggcacag caactgggcg gcccgacgga agtgcgcgtt gaggtcaaca
     8221 aaagttcgcc tgtcgtctgg ctgaaaacct ggctgtcgcc caatatctgg gtacgcgtgc
     8281 cgctgaccga aattcatcag ggcgatttct ctccgctgtt ccgctatacg ctggcgatta
     8341 tgctattggc gataggcggg gcgtggctgt ttattcgtat ccagaaccga ccgttggtcg
     8401 atctcgaaca tgctgctttg caggtcggta aagggattat tccgccgccg ctgcgtgagt
     8461 atggcgcgtc cgaggtgcgt tccgttaccc gcgcctttaa ccatatggcg gctggtgtta
     8521 agcaactggc ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc
     8581 cgctgacgcg tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag
     8641 aatcgatcaa taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc
     8701 tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct taatgcagtg ctcggtgagg
     8761 tgattgctgc cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca
     8821 ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg
     8881 ccgctcgtta tggcaatggc tggatcaaag tcagcagcgg aacggagccg aatcgcgcct
     8941 ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc
     9001 agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa
     9061 ttgtgcagcg tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg
     9121 gcgggctttc cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcacgacaa
     9181 aagaagggta aataaacggg aggcgaaggt gcctcccgtt ttgctttcta taagatactg
     9241 gatagatatt ctccagcttc aaatcattac agtttcggac cagccgctac cagcgcggca
     9301 cccgcagggg tgtcggtgta tttatcgaag ttgtcgataa acagtttcgc cagggtttcg
     9361 gctttttcct gccactgttc cggagaagcg taggtgttac gcggatcgag aatcttcgtg
     9421 tctacgcccg gcagttcggt tgggatcgcc aggttaaaca tcggcagagt gaaggtttct
     9481 gcattatcca gcgaaccgtt gaggatggcg tcgataatgg cgcgggtatc tttaatcgag
     9541 atacgtttgc cagtgccgtt ccagccagtg ttaaccagat aagcctgcgc gcccgccgcc
     9601 tgcatacgtt tcaccagcac ttctgcgtac tgagtcgggt gcagcgacag gaacgccgcg
     9661 ccgaagcaag cggagaaggt tggcgtcggt tcggtgatgc cacgctcagt accggccagt
     9721 ttggcggtga agccagagag gaagtgatac tgggtttgat cggcagtcag gcgagaaacc
     9781 ggcggcaaca cgccgaaagc atcagcagtc aggaagataa ccttagtcgc gtggcccgct
     9841 ttggaaaccg gcttaacgat gttatcgatg tgatagattg gataagaaac gcgggtgttc
     9901 tcggtttttg aaccatcatc aaagtcgata gtgccatctt cacgcacggt gacgttttcc
     9961 agcagcgcat cacgacggat agcgttgtag atttcaggtt ccgcttcttt cgacagcttg
    10021 atagtttttg cgtagcagcc gccttcgaaa ttaaacacgc cgtcatcgtc ccagccgtgt
    10081 tcgtcatcgc caatcaggcg gcgtttcggg tcggtggaaa gggtggtttt accggtgccg
    10141 gaaaggccga agaacaccgc aacatcgcct ttctcgccaa cgttggcgga gcagtgcata
    10201 gaagcgatac ctttcagcgg cagcaggtag ttcatcatcg agaacatccc tttcttcatt
    10261 tcgccgccgt accaggtgcc gccaatcagc tgcatgcgct cggtcaggtt aaacgccacg
    10321 aagttttcgg agttcagacc ctgttctttc cactgcgggt tagtgcactt cgcgccgttc
    10381 ataacgataa agtctagttt gaaacctgcc agctcttcat cgctcgggcg aataaacatg
    10441 tttttgacaa aatgcgcctg ccaggccact tcggtgatga aacggacgga aagacgagta
    10501 tccgggttcg caccacagaa agcgtcgaca acgaacagac gtttgccgga aagctgcctg
    10561 gtcaccaggc ctttcagatg ctgccaggtt tccggagaga gaggtttgtt gtcgttctta
    10621 cctttgcctt tgtctgccca ccagaaagta tcgcgagtgg tatcgtcacg gacgatatac
    10681 ttatcttttg gtgaacgacc ggtgaagatc ccggtatcga cggcaacggc acccagatta
    10741 gttaacaccc cgcgctcata acctgtcagg ctcggatcga gctcttcctg atacagcagg
    10801 tcgtagcttg ggttgtaaac gatatcatgt acgtcactga taccataagc ctcgagttct
    10861 tgcggggtca aaccattgtt aacgcgcatt tcactgctcc ttagccaata tgtattgcct
    10921 gaatagtaaa gtctttttgg gggtgttaac cgcgacaagg ctcatagatt tacgtatctg
    10981 gagaaattca tatttgacaa aaaactccgt gattcctgtc acgaaacggt tgctattatc
    11041 gcagaaatag cattttaggt gtggaaaatg ttctcaaaag gttaaatctt ggtttatatg
    11101 gcgggaatat tgtgagtgga atcgcattcc tgaatgaaat tgatgcaaat tcaaccggta
    11161 aagtatcgat tcaccacgct aacttttttt gattattttc tgaaaaaagc aaaatactac
    11221 tgcccggatg cgataattaa cccacctttt gattctggat aaccaacatg gataacgttg
    11281 aactttcacc ggcaacacgc tgggggatga ttgctaccgg attacttcag gggctggttt
    11341 gctacctgct tatcgcctgg cttgccggaa aaaatcacag ttggattgtt tatggcgtgc
    11401 ctgcgaccgt cgcgttttca tccgttttac tcttctccgt gatctctttt aaacagaagc
    11461 gcctttgggg atggctggcg ctggtgttta ttgccacgac agggatgagc ggttggctga
    11521 agtggcaaac tgatggcatg aacccctgga gagctgaaaa ggcgatttgg gattttggct
    11581 gatatctgct gttgatggca atgttgttgc taccgtggat acaacaaagc cttcgtatcc
    11641 gcaatggcag tagccgctat agctactttt accagtcagt atggcataac gtacttatat
    11701 tactggtgat ttttctctcc aatggcctga cgtggctggt gcttttactg tggggtgagt
    11761 tgtttaaact tgttggcatc acgtttttca atacgctctt ttttgcaacc gactggttta
    11821 tgtatcttac atttggtctg gttaccgcac tggtggtgat cctcgcgcgt acacagtcac
    11881 gtttaatcga ctctattcaa aagttgttca cgctaatcgc cacggggttg ctgccgttag
    11941 tatcattgct aaccctgatg tttatcatca ccctgccgtt tacggacctg agcgcgattt
    12001 ctcgccacat ctccgccgcc gggttgctgt taacgctggc ctttttgcaa ttgatcttaa
    12061 tggctattgt ccgcgatccg caaaaagcgt cacttccctg gacggggccg ttgcgttgcc
    12121 tgattaaaac cgctttgctg gttgctccgc tgtatgtgtt cgtcgccgcc tgggcgttat
    12181 ggctgcgggt cgctcagtac ggctggactg tcgaccgctt gcagggcgcg ctggcggtgc
    12241 tggtgttact ggtgtggtcg ctggggtatt ttgtcagtat cgtctggcgt aatgggcaaa
    12301 atccccttgt tcttcagggc aaagtgaacc ttgcggtttc gttattggtg ttggtgatac
    12361 tggtgcttct taattcgccg gtgctggaca gtatgcgcat tagcgtgaac agtcatatgg
    12421 cgcgttatca gagcggcaaa aacacgccag accaggtaac tatctacatg ctcgaacaga
    12481 gcggtcgcta tggacgtgcg gcgcttgagt cgctgaaaag cgatgccggg tttatgaaag
    12541 acccgaaacg cgcgcgggat ctgctgatgg cgttagatgg agagcaacat cttcaggaac
    12601 aggtatcgga aaaagtatta gccgaaaatg tgttaattgc tcctggttct gttaaaccgg
    12661 atgcgacatt ctggtcggcc ttaatccagg atcgctataa cgtgatgacc tgtattgaaa
    12721 aagacgcctg cgtcctggtc gagcaagatc tgaatagtga tggtcaggcg gagcggatcc
    12781 tgtttgcttt taatgatgac agagtcattg tctatggctt tgactcagac agaaaagaat
    12841 gggacgcgct tgatatgagt ttacttccga acgaaataac gaaagaaaaa ttactcacag
    12901 ctgcgaagga tggaaaactg gatctgacgg tagatggtga aacgctggaa ataaacttaa
    12961 gtaagtgaca actcttttaa cggatacgac gctgtatctg accaacaata atttgcgatt
    13021 tatttcacag gcctggtaag cgcagcacat caggccatta cgtcgtaaaa tccggcagag
    13081 ccctctctgc cggacatact cattaatgaa cttgcggatc tgccggagac gcgttgttgc
    13141 ggatttcagc aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca
    13201 tgtcaatttc gccatcttcc gccaggatgc tatcgacttc ttcatcaggc agcgttttca
    13261 gcgcgtcggc gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt
    13321 aaaccgtcac ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca
    13381 gcagttcttc ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgct
    13441 gggcattttg cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta
    13501 cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt
    13561 ccaggcaggc cgccagggta tcaccttcca gaccaaccac gccctgatag cgttcgcctt
    13621 cgctcggggt aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat
    13681 tttctggaat ttcgccctgc acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt
    13741 taataaccgc cagattcatc ggaccgtcgc cttgcagctg tacggtgata tcaccatcaa
    13801 acttcagcgt agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa
    13861 cgggctgcgg ataatcgtga ttctcaagga tctgttgcag ggtttccgaa acggttacca
    13921 gttcgccgcg cacggcaaag ttttcaaaca gatagcgatg taattggtca tgttgcggca
    13981 taatcatctc tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt
    14041 cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt
    14101 tacgtgccag cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca
    14161 aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt
    14221 cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc
    14281 tgcgctgccc gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg
    14341 ttttataaaa acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggttttt
    14401 ctttcatggc gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac
    14461 ggatggcgtt gatactgttt ctcggcaatc ccggaatcag gattagtcac gccgaggcag
    14521 taacgaatac caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc
    14581 gttctttcag ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct
    14641 ttcggataac caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagaccggta
    14701 tgctcaagtt ttaccgccag gttgtgcgga tgcgcattgg tgagcaaaat tcgctgctta
    14761 ccgctggctt tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcacgcggt
    14821 cccatctcgg tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga
    14881 cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct
    14941 tcctgtggcg taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa
    15001 tagttatcga aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg
    15061 tcctgccagg caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga
    15121 cagggtagca taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat
    15181 cagttgcggg ttcaggcagc tttcataata ggcctgaatc ttcatcatgc gagtgcgatg
    15241 acgctggtaa cgacgccagg cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag
    15301 tagcagcaat gtggtgctga gatagcgcca caggccggaa cgatccggga ttggatgaag
    15361 gccaatatgc tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cctcggcgtt
    15421 aaacggcgta tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg
    15481 cgggtagtcg taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc
    15541 gctgacaatc aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt
    15601 ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg ttatccagcg attccgcact
    15661 caccgggcgt aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag
    15721 cgcgtcccag tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc
    15781 atctttggca gaacataaat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa
    15841 taccatgccg gatttttgaa ttgctgaacg taatgaggca ctgacgcgag atccatcttc
    15901 cggtttaggg tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag
    15961 ttcagattct ggtaatggca atgagcgggc gtcgttccag atgatctgcg agcagtcaaa
    16021 cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat
    16081 tcccgtaccg ctgatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt
    16141 tacgctggtg gcttcaatgg tctgtgcgcc tttcatccat gagagagtga atttcagcgg
    16201 catatccagc gggatccaga ataacagcat aaacagcacc agcagcgagc ccgcagcgat
    16261 aatcgtactg cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag
    16321 atatcgcccc tgacgcacta cgtggcggtc gagatagata tcaatatcgg tttgttgacc
    16381 gagatcctga gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag
    16441 tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt
    16501 accgcgcaga caatgaattt cccgcaggga ggattttgcc gggggcgcga atagccccca
    16561 cagacctgcg cccagcagca gtaatgcgcc gcctgccagc cacggaacaa atacatccgg
    16621 ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag
    16681 cccgcgcgga cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc
    16741 gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc
    16801 atagccgcgc gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccag
    16861 cggcagcgaa tcggtataaa tcagttcaac ggtattctca tcgttgatgt attgttccca
    16921 gaaagggggc aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc
    16981 ggtagagatg ccgtaacgcg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc
    17041 attcagcgcc agagagatcg gtgccgcgct agctcccgtt gggccaggca cctgtaatac
    17101 ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg
    17161 cgtttgcgca tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt
    17221 tatcagccat cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt
    17281 gctcatgctt tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cagacacaat
    17341 acatgattct gcggttggct atcggcaagc gtggagttta acttgagcat cattaagcac
    17401 atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg ggatatcagc aaattcctgg
    17461 agttatttca acctattgac ttttaatttg atttgaggac aattatttat taagttacat
    17521 aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg
    17581 ctgatttcac agcatagatc ccacgatgag caaatcatta caaaaaccca ccattctgaa
    17641 tgttgaaact gtagcccatt cccgactgtt taccgtcgag agcgtggatc tggagttcag
    17701 caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat
    17761 tgtgccgatt gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc
    17821 ctacgaatta ggtttttcga aaggattaat tgatccgggt gaaagcgtct acgaagccgc
    17881 taaccgcgag ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa
    17941 gctcagcatg gcaccgtctt acttttccag caaaatgaat atcgtggtcg cgcaagatct
    18001 ctacccggaa tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct
    18061 ggcgcatatg atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc
    18121 gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg
    18181 cctgatgcac aacgattatc aggcttatgg ggttcctgca atatattgat ttttcacgat
    18241 tttgttagcc ggataacgcg ttcacccgca tccggcataa acaaatgcac tttgtcagcc
    18301 aactgaaaag gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct caccattatc
    18361 gataatggtt gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa
    18421 atactcttcg cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac
    18481 cgtcacaata cccggaggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt
    18541 catataagcg tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg
    18601 atctttaatc gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc
    18661 aatccagacc gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact
    18721 gttagtggta ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca
    18781 gccggtaccc tgccagcctg gttcaccaaa gatattggtg ttcaaagcac tcttaatcag
    18841 gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac
    18901 taacgcctga tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg
    18961 ggagatagcg acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc
    19021 gtaaatcacc ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac
    19081 gccgccctga tcgttttcaa ttttgctgat aaaccacggg tccaccagga agccgccgtt
    19141 cgccatgacc gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc
    19201 cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc
    19261 agcgtagtcg acgcccatcg cccgcattgc acgtaccatc accacgtttt tcgactgacc
    19321 cagcccctga cgtaagcgaa ttggaccagc atactgcggt ggtgagttct tcggctgcca
    19381 gtcagaaccg acacctgcat cccagcgaga aattggcaca tcgttcaaca tacttgccag
    19441 cgtcagacct ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg
    19501 acgcagtgcc tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag
    19561 cgccataacg gcaccatttt gcggattgat cgacaccagc gccgagttca cttccggcac
    19621 ttgtgccagc caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag
    19681 aacatcggtc actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc
    19741 ccagcgaacg ccgtccatac tcaatgcgac agtcgacccg tccgccagca ttgccgtcgc
    19801 ttcctgagga ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc
    19861 cttcagcgta tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac
    19921 atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc
    19981 ctgctgtgcg gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc
    20041 ataggcactt tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg
    20101 cgcagagaaa gcaatctccg gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg
    20161 atcgaactgt tgttgggtga tatacccttc atccagcatc cgcgacagta cgacgttacg
    20221 ccgcgcgacg gcacgatcca ttgagtagag cggattaaag gtggaaggcg ctttcggcag
    20281 cccggctatc acagccattt cgttcagcgt cagttggtcg accgttttac cgaaatagac
    20341 ttgtgccgca gcaccgacac cataggcgcg gtaaccgagg taaatcttgt tcagataaag
    20401 ctcgaggatc tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt
    20461 aatcttacgc atcagcgtgc gttctggact gaggaagaag tttctcgcca gctgctgggt
    20521 aatggtactc gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa
    20581 gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa
    20641 ggctttcacc atctccggcg ggatttgatc caacgtaacc ggaatacgac gtttctcacc
    20701 gtattgagca atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac
    20761 atcttttaat gtcgccacat ccggcagttg tggctcgatg tagcggtata ggccataaat
    20821 cgagcctgct cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt
    20881 cactggaaat ttcccattta gtttcatttg ggcagtttat aaacaaacgt gcggtagtat
    20941 aaaggcaagc cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca
    21001 caatggcatt taagatctgg caaattggtt tgcatttaca acagcaagaa gcagtagcgg
    21061 ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg
    21121 agaacgacat tatcaaagat gggcggattg ttgatgcgca gcggctggct aaaacgttgt
    21181 taccgtggag tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc
    21241 gcacattaca gcggtcattt ccgcgcccgt cgatgtccct cggtgagcgg gagcaaacgg
    21301 cctggctgtc agggacgatg gcccgcgagc tggatatgga tccggactcc ctgcgtttcg
    21361 attacagcga agactcactc agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc
    21421 tggcaacgct actcactctg gcagaaaggt tgcgtgttca tgtgagcgcg atcaccccgg
    21481 atgccagtgc attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct
    21541 ggcgtgataa cgaacagtgg ctgtgggcga cacgctgtcg ctgggggcgc aaactggcgg
    21601 tggggatgac tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gaaagcgtcg
    21661 cgatatgtgg cgaaggcgga tttgatccct gggaggccgt ttctgttcgt cagccgccgc
    21721 taccgccgcc aggtggagac tttgccatcg cgctggggct ggcgcttggg aaggcgtact
    21781 gatgaacccg ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttcttcg
    21841 tttctggttg ctgatgttcg ttgcgcctct gctgctggcc gtcgggataa cgctaatact
    21901 gcgtctgaca gacaacgccg aagcacgcgt aaacaccgtt ttgcttcagg cggaacaaca
    21961 actcgcccgc agcttacaga taacgaaacc acgtttgctg gagcgccaac aaattcgtga
    22021 gcagcgtttg caacggcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga
    22081 agcactggcg gcgcttttac ccgagcacgc ctggctgaca acgataagct ggcagcaggg
    22141 aacgctggag atcaaggggc tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc
    22201 actccgccag gatgcgtctt ttcatctcaa tcagcgggga gccacgcagc aggatgcgca
    22261 gggacgctgg caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg
    22321 actggtggtt cgccacatca ccccgcctcc gccagttttg ctgggcattc tggttgctga
    22381 tgttagttac gctcattttt ctgtcatcga cacaccatga agagcgcgac gcattaattc
    22441 gactacgggc aagtcatcac cagcagtggg ccacactgta tcgcctggtt gacaccactc
    22501 ccttcagcga agaaaaaacg ctgccctttt cgccactgga ttttcagttg cccggcgcgc
    22561 aactggtttc ctggcatcca tccgcgcagg gaggcgagtt ggcgttgaaa acgctgtggg
    22621 aagcagtgcc gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt
    22681 cattaagcgt ggaaggtgat gatcttttgt tcacgctaca actggagacg ccgcatgagg
    22741 gttaaacgct ggttgttggc aggtattgta ttgtgccttt taaccggtat gcgtgaccct
    22801 tttaaaccgc cggaagatct atgccggatt agcgaactta gccagtggcg ctatcagggg
    22861 atggtagggc gaggcgagcg catcatcggt gtaataaaag acgggcaaaa gaaatggcga
    22921 cgggtgcagc aaaacgatgt gctggaaaac ggctggacaa ttttacagct gacgccagac
    22981 gtactaacgc tgggtaccgg gacaaactgc gaaccgccac aatggttgtg gcaacggcaa
    23041 ggagatacaa atgaagcaat ggatagccgc actactgttg atgctgatac ccgccgtaca
    23101 ggcggcaaag ccgcaaaaag tgacgctgat ggtggatgac gttccggtag ctcaggtgtt
    23161 gcaggcgctg gctgaacagg agaagttgaa cctggtcgtg tcgccagacg tcagcggtac
    23221 ggtgtcgtta catctaacag atgttccctg gaagcaggca ctacaaactg tagtgaaaag
    23281 cgccggactg ataacgcggc aggaaggcaa cattctctca gtgcattcca ttgcctggca
    23341 gaataacaat atcgcccgcc aggaggcgga gcaggcgcgg gcgcaggcaa atctgccgct
    23401 ggaaaatcgc agtataaccc tgcaatacgc cgacgcggga gaactggcga aagcggggga
    23461 gaagctactg agtgccaaag ggagtatgac cgtcgataaa cgcaccaatc gccttttgct
    23521 acgagataac aaaacggcgt taagcgcgct tgaacagtgg gtagcgcaaa tggatctgcc
    23581 ggtcgggcag gttgagctgt cggcgcatat tgtcaccatt aatgaaaaaa gtttgcgtga
    23641 gttaggcgtg aaatggacgc tggccgatgc gcaacacgct ggtggcgttg ggcaagtcac
    23701 cacgcttggt agcgacctct ccgtagcgac ggcgacaacg catgtcggtt ttaacattgg
    23761 gcgcatcaac ggacgcttgc tggatcttga gctttccgcg ctcgaacaaa aacagcagct
    23821 ggatattatc gccagtccgc gtctgctggc ctcacatctt cagcctgcca gcattaaaca
    23881 ggggagcgaa attccatatc aggtttccag cggggaaagt ggcgcgacgt cggtggaatt
    23941 taaagaggcc gtcctgggga tggaagttac gcccacagtg ttacaaaaag gtcgcatccg
    24001 gctgaaatta cacatcagcc agaacgttcc ggggcaggtg ctacagcagg ccgatggcga
    24061 agtgctggcg attgataagc aggagatcga aacgcaggtc gaggtcaaaa gcggagaaac
    24121 gttggcgctg ggcggcattt ttacccgtaa aaataaatcg ggtcaggata gcgtaccgtt
    24181 gcttggcgac attccctggt tcgggcaatt atttcgtcat gacggaaaag aagatgaacg
    24241 acgcgagtta gtggtgttta tcacgccacg actggtttcc agtgagtaaa cagccgtaaa
    24301 agcggtaatg tttttacgct gaacgtgttt catctatttg acgcgcgcag gtatttagca
    24361 tacaaggagt accgatttga gagttggtgc tcttcgctgc ctgcgttcca tgatgatgat
    24421 ttatcattca ggcggcattt tgctgtcttt tttacgctaa tcttacccgg tgatttatcg
    24481 ccagagcggt ggtagcaagg cagcgcgctt gcagcgacca gatatgcaga gggatgggtg
    24541 atttattcag ttgccaaacc cgctggagta ttgagataat tttcagtctg actctcgcaa
    24601 tatcttatga ggtttcagtt catgtcctgc ggcgctctct gagcgaagcg ggtttatcat
    24661 taacgaatag tcttagtagt accgaaaaaa tggcagagaa acgcaatatc tttctggttg
    24721 ggcctatggg tgccggaaaa agcactattg ggcgccagtt agctcaacaa ctcaatatgg
    24781 aattttacga ttccgatcaa gagattgaga aacgaaccgg agctgatgtg ggctgggttt
    24841 tcgatttaga aggcgaagaa ggcttccgcg atcgcgaaga aaaggtcatc aatgagttga
    24901 ccgagaaaca gggtattgtg ctggctactg gcggtggctc tgtgaaatcc cgtgaaacgc
    24961 gtaaccgtct ttccgctcgt ggcgttgtcg tttatcttga aacgaccatc gaaaagcaac
    25021 ttgcacgcac gcagcgtgat aaaaaacgcc cgttgctgca cgttgaaaca ccgccacgtg
    25081 aagttctgga agcgttggcc aatgaacgca atccgctgta tgaagagatt gccgacgtga
    25141 ccattcgtac tgatgatcaa agcgctaaag tggttgcaaa ccagattatt cacatgctgg
    25201 aaagcaacta attctggctt tatatacact cgtctgcggg tacagtaatt aaggtggatg
    25261 tcgcgttatg gagaggattg ttgttactct cggggaacgt agttacccaa ttaccatcgc
    25321 atctggtttg tttaatgaac cagcttcatt cttaccgctg aaatcgggcg agcaggtcat
    25381 gttggtcacc aacgaaaccc tggctcctct gtatctcgat aaggtccgcg gcgtacttga
    25441 acaggcgggt gttaatgtcg atagcgttat cctccctgac ggcgagcagt ataaaagcct
    25501 ggctgtactc gataccgtct ttacggcgtt gttacaaaag ccgcatggtc gcgatactac
    25561 gctggtggcg cttggcggcg gcgtagtggg cgatctgacc ggcttcgcgg cggcgagtta
    25621 tcagcgcggt gttcgtttca ttcaagtccc gacgacgtta ctgtcgcagg tcgattcctc
    25681 cgttggcggc aaaactgcgg tcaaccatcc cctcggtaaa aacatgattg gcgcgttcta
    25741 ccagcctgct tcagtggtgg tggatctcga ctgtctgaaa acgcttcccc cgcgtgagtt
    25801 agcgtcgggg ctggcagaag tcatcaaata cggcattatt cttgacggtg cgttttttaa
    25861 ctggctggaa gagaatctgg atgcgttgtt gcgtctggac ggtccggcaa tggcgtactg
    25921 tattcgccgt tgttgtgaac tgaaggcaga agttgtcgcc gccgacgagc gcgaaaccgg
    25981 gttacgtgct ttactgaatc tgggacacac ctttggtcat gccattgaag ctgaaatggg
    26041 gtatggcaat tggttacatg gtgaagcggt cgctgcgggt atggtgatgg cggcgcggac
    26101 gtcggaacgt ctcgggcagt ttagttctgc cgaaacgcag cgtattataa ccctgctcaa
    26161 gcgggctggg ttaccggtca atgggccgcg cgaaatgtcc gcgcaggcgt atttaccgca
    26221 tatgctgcgt gacaagaaag tccttgcggg agagatacgc ttaattcttc cgttggcaat
    26281 tggtaagagt gaagttcgca gcggcgtttc gcacgagctt gttcttaacg ccattgccga
    26341 ttgtcaatca gcgtaacaac aagaaaggtc aggccgctta tcaagcggtc tattagcttc
    26401 aggttaattg caacgtggta agcattaacc ttttagtggg gtgttaaatg gatgaattca
    26461 aaccagaaga cgagctgaaa cccgatccca gcgatcgtcg tactggtcgt tctcgtcaat
    26521 cttctgaacg ttctgagcgt actgaacgtg gcgaaccgca gatcaatttt gatgatattg
    26581 aacttgatga cactgacgat cgccgtccga ctcgtgcgca aaaagagcgc aatgaggaac
    26641 cggaaatcga agaagaaatt gacgaatccg aagatgaaac cgtggatgaa gagcgcgtag
    26701 agcgtcgtcc gcgtaagcgc aaaaaagcag ccagtaaacc cgcttctcgt cagtatatga
    26761 tgatgggcgt cggcattctg gttctactgc tgttgatcat cggtatcggt tctgcgctaa
    26821 aagccccctc gacctcttcc agcgatcaaa ccgcgtctgg cgagaagagt attgatcttg
    26881 caggcaatgc gaccgatcag gcgaatgggg tgcagccagc gccgggaacc acgtctgcgg
    26941 aaaatactca gcaggatgtt tctctgccac cgatctcttc tacgccgact caagggcaaa
    27001 ccccggcggc aacggatggt caacaacgtg ttgaagtgca gggtgacctg aacaatgcgc
    27061 tgacccagcc acaaaatcag caacagttga acaatgtggc ggtcaattcc acattgccga
    27121 ccgaacccgc gactgtcgcg cctgttcgca atggcaatgc atcgcgtgac acggcgaaaa
    27181 cgcaaaccgc tgaacgtccg tccactacgc gcccagctcg tcagcaggcg gtgattgaac
    27241 cgaaaaaacc gcaagcaacc gtgaaaacgg agccgaagcc ggtagcacag acgccgaagc
    27301 gtactgaacc agctgcccct gtggcgagca cgaaggcacc gactgcgact tctacgccag
    27361 caccaaaaga gacggcgact acggctccag tacagacggc atccccggcg caaaccacgg
    27421 caacaccagc cgctggaggg aagaccgcag gtaatgttgg ttcgttgaaa tcggcaccgt
    27481 ccagccatta cactctgcag ctgagcagtt cctctaacta cgacaacctg aacggttggg
    27541 cgaagaaaga gaatctgaaa aactacgttg tctatgaaac ggcgcgtaat ggtcagccgt
    27601 ggtatgtcct ggtttctggc gtgtacgctt cgaaagaaga ggcgaaaaaa gcggtatcta
    27661 cattgccagc agatgttcag gccaaaaacc cgtgggcgaa accgctgcgt caggtacagg
    27721 ccgatctgaa gtaatcaagg ttatctcccg caatggttta tcgttgcggg agctgcctga
    27781 agcgctggat gctgtcggag ctttctccac agccggagaa ggtgtaatta gttagtcagc
    27841 atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct gcttgatgat
    27901 attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg
    27961 gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag cgacctgatc
    28021 agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc acgcgagctg
    28081 tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa
    28141 agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta cggttacaac
    28201 ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgtttggccg ctacaaaaaa
    28261 acctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc
    28321 tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt cgtctattgc
    28381 gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca cacaaacagt
    28441 tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt tgagcgccat
    28501 attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta tcagcgcgca
    28561 aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac acgtaaaaag
    28621 gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa aaaataattc
    28681 tcaaggagaa gcggatgaaa cagtatttga ttgccccctc aattctgtcg gctgattttg
    28741 cccgcctggg tgaagatacc gcaaaagccc tggcagctgg cgctgatgtc gtgcattttg
    28801 acgtcatgga taaccactat gttcccaatc tgacgattgg gccaatggtg ctgaaatcct
    28861 tgcgtaacta tggcattacc gcccctatcg acgtacatct gatggtgaaa cccgtcgatc
    28921 gcattgtgcc tgatttcgct gccgctggtg ccagcatcat tacctttcat ccagaagcct
    28981 ccgagcatgt tgaccgcacg ctgcaactga ttaaagaaaa tggctgtaaa gcgggtctgg
    29041 tatttaaccc ggcgacacct ctgagctatc tggattacgt gatggataag ctggatgtga
    29101 tcctgctgat gtccgtcaat cctggtttcg gcggtcagtc tttcattcct caaacactgg
    29161 ataaactgcg cgaagtacgt cgccgtatcg acgagtctgg ctttgacatt cgtctggaag
    29221 tggacggtgg cgtgaaggtg aacaacattg gcgaaatcgc tgcggcgggc gcggatatgt
    29281 tcgtcgccgg ttcggcaatc ttcgaccagc cagactacaa aaaagtcatt gatgaaatgc
    29341 gcagtgaact ggcaaaggta agtcatgaat aagtttgaag atattcgcgg cgtcgctttt
    29401 gatcttgatg gtacgctggt cgacagtgct cccggtcttg ctgctgcggt agatatggcg
    29461 ctgtatgcgc tggagttgcc catcgcaggt gaagaacgcg ttattacctg gattggtaac
    29521 ggcgcagatg ttctgatgga gcgcgcattg acctgggcgc gtcaggaacg tgcgactcag
    29581 cgtaaaacaa tgggtaaacc gcccgttgat gacgacattc cggcagaaga acaggtacgt
    29641 attctgcgta aactgttcga tcgctactat ggcgaggttg ccgaagaggg gacgtttttg
    29701 ttcccgcacg ttgccgatac gttgggcgcg ttgcaggcta aaggcctgcc gctaggcctg
    29761 gtcaccaaca aaccgacgcc gttcgtcgca ccgctgctcg aagccttaga tattgccaaa
    29821 tactttagcg tggttatcgg cggcgatgat gtgcaaaaca aaaaaccgca tccggacccg
    29881 ctgttactgg tggctgagcg gatgggaatt gccccacaac agatgctgtt tgtcggcgac
    29941 tcacgcaatg atattcaggc ggcaaaagcg gcaggttgcc catcagttgg cttaacctac
    30001 ggatataact acggcgaggc tatcgatctc agccagcctg atgtaattta tcagtctata
    30061 aatgaccttc tgcccgcatt agggcttccg catagcgaaa atcaggaatc gaaaaatgac
    30121 taagcccatc gtttttagtg gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat
    30181 gggtgcgctg cgtcagtggg taaacatgca ggatgactac cattgcattt actgtatcgt
    30241 tgaccaacac gcgatcaccg tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga
    30301 tacgctggcc ttgtatctgg cttgtggtat cgatcctgag aaaagcacca tttttgttca
    30361 gtcccacgta ccagaacatg cgcagttagg ctgggcactg aactgttata cctacttcgg
    30421 cgaactgagt cgcatgacgc agtttaaaga taaatctgcg cgttatgccg agaacatcaa
    30481 cgctggtctg tttgactatc cggtgctgat ggctgcggac atcctgctgt atcaaactaa
    30541 tctggtaccg gtgggtgaag accagaaaca gcacctggaa ctgagtcgcg atatcgccca
    30601 gcgtttcaac gcgctgtatg gcgagatctt taaggtgccg gagccgttta ttccgaaatc
    30661 tggcgcgcgc gtaatgtcgc tgctggagcc gaccaagaag atgtccaagt ctgacgataa
    30721 ccgcaataac gttatcggcc tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg
    30781 cgcggtcact gactccgacg agccgccggt agttcgctac gatgtgcaga acaaagcggg
    30841 cgtttccaac ctgctggata tcctttctgc ggtaacgggc cagagcatcc cagaactgga
    30901 aaaacagttc gaaggcaaga tgtatggtca tctgaaaggt gaagtggctg atgccgtttc
    30961 cggtatgctg actgaattgc aggaacgcta tcaccgtttc cgcaacgatg aagccttcct
    31021 gcaacaggtg atgaaagacg gcgcagaaaa agccagtgta cacgcttccc gtacgctgaa
    31081 agcggtgtac gaagcgattg gttttgtggc gaagccgtaa tttaacatta atgccaaaaa
    31141 ccggggaaac ccggtttttt tatcctcatt tgcaataatc cgaaaaaatg tgaagcgcct
    31201 cgccgtttcc acatctcgtc gttgccactt atcttcattt taatgaagat ataaatattc
    31261 attttattga aaatatatta tgcgtcgaac gtttatcaaa aaagaaggcg tcgtcatcac
    31321 gacgctggcc cgttatttgt tgggtgaaaa atgcggtaat cgactgaaaa ccatagatga
    31381 gctggcaact gaatgccgtt catcggttgg cctgacgcag gccgcgttga aaacgttgga
    31441 atcaagcgga gcgatacgga ttgaacgccg tgggcgcaat ggcagttatc tggtcgagat
    31501 ggataacaaa gcattgctga gtcatgtgga tatcaacaac gtggtatgtg cgatgccttt
    31561 gccctatacc cgcttgtacg aaggtttagc gagcgggctg aaagctcagt ttgatggcat
    31621 tcctttttac tatgcgcata tgcgcggcgc ggatattcgc gtggagtgtt tacttaacgg
    31681 cgtgtatgac atggcggtgg tttcccgact ggcggcggaa agttatctca cgcaaaaagg
    31741 gttatgcctc gcgctggagt tggggccgca cacctacgtt ggcgagcacc agttgatttg
    31801 ccgtaaaggc gagtccgcaa acgtgaagcg cgtggggctg gataaccgtt cggcggatca
    31861 gaaaatcatg accgatgtct tttttggcga tagtgatgtg gaacgtgtcg atctctctta
    31921 tcacgagagt ttacaacgca ttgttaaagg cgatgtcgat gcggttatct ggaacgtggt
    31981 ggcggaaaac gaactgacca tgctgggatt agaggcgacg ccgctcacag acgatccgcg
    32041 atttttacag gccaccgaag ccgtggtcct gacgcgagtc gatgattacc caatgcaaca
    32101 actgctgcgt gccgttgtag ataaacacgc cctgctcgcc catcaacaac gggtagtgag
    32161 tggggaacag gaaccgagtt attaaacgaa aggcatctga tatggaaacc agactcaacc
    32221 tgctttgcga ggcaggcgtt attgataagg acatctgcaa aggcatgatg caggtcgtca
    32281 acgtactgga aacagagtgc catctgccgg tgcgcagtga gcaaggaacg atggcgatga
    32341 cacatatggc gagtgcactg atgcgcagtc gccgtggtga agaaatagag ccgctggata
    32401 acgaattgct ggcagagctg gcgcaatcca gccactggca agccgttgtg caattgcatc
    32461 aggtgttgtt gaaggaattc gcactggaag ttaacccgtg tgaagaaggc tatttgctgg
    32521 ccaaccttta tggattatgg atggctgcta acgaagaggt ttgaagctcc cgacaaatcc
    32581 acgtcgcaca acctaaatat tcaaaaaaat gaatatttaa ttcaaggaac agaaacgagg
    32641 caacaagatg tttgtagaag cattgaaacg ccagaacccg gcgctgattt ccgccgcact
    32701 aagtttgtgg cagcagggca agatcgcccc ggacagctgg gtgatcgacg tggatcaggt
    32761 actggaaaac ggtaagcgac tgattgagac ggcgcggctt tacggcattg aactgtatct
    32821 gatgaccaag cagtttggcc gtaacccatg gctggcggaa aaattgctgg cattaggtta
    32881 cagcggcatt gtggcggtgg attacaaaga ggcgcgagtc atgcgccgcg ctggtttgtc
    32941 ggtggcacat caggggcatc tggtacaaat cccttgtcat caggttgctg acgccgttga
    33001 acagggcacc gacgtcatca ccgtgtttac tctcgacaaa gcgcgggaag tttctgcggc
    33061 agcggtgaag gccggacgtg tgcagtttgt gctgctcaaa gtttatagcg acgatgattt
    33121 tctttatccg gggcaggaga gcggttttgt acagcattcg ctgcacgagg tggttgctga
    33181 aattaagaaa ctgccggggc tgcatttagc cggacttacc catttccctt gcctgctttg
    33241 ggatgaggct gccggaaaag ttttgccgac accgaatctt cacacgctga tacaggcacg
    33301 ggatcaactg gcgaaatctg gtattgcact tgagcaactg aacgcgcctt cagcgaccag
    33361 ctgcacttcg ctgccattac tggcgtaata cggtgtgact catgccgaac ccggtcatgc
    33421 gctgacgggc actattccgg caaaccagca gggcgatcag tctgaacgta tcgcgatgct
    33481 ctggttgagt gaaatctccc atcatttccg tggcgacagc tactgctacg gcggcggtta
    33541 ctaccgtcgt ggtcatgcgc aacatgcgtt ggtgtttacg ccagaaaatc aaaagattac
    33601 tgaaaccaat ctcaaaactg tggatgacag cagtatcgac tacaccctgt cgctggcagg
    33661 cgagtttccg gtaagcagtg cagtggtgct ctgtttccgc acgcagattt ttgtcacccg
    33721 tagcgatgtg gtgctggtgt ccggtattca ccgtggcgaa ccgaaaatag ttggtcgtta
    33781 tgacagtctt ggaaactctc tgggggcgta atggcgcgat ttgtggtgtt agtgattgat
    33841 agctttggcg taggggcaat gaaagatgtc acgctggtgc gtccgcaaga tgcgggagcg
    33901 aatacatgtg gtcacatcct gagccagttg ccgcatttgc agctaccaac gctggagaag
    33961 ctggggctaa tcaacgcatt gggttatgcg ccaggcgata tgcagccgtc agattccgca
    34021 acctggggcg tggcagagct gcaacatgaa ggtggcgata cctttatggg gcatcaggaa
    34081 attttaggca cgcgcccgtt accgccgctg cggatgcctt ttcgcgatgt gattgaccgt
    34141 gttgagcagg cattagtatc cgccggttgg caggtggagc gccgtggcga tgaactgtaa
    34201 tttctgtggg tcaatcaggc ggttgcgatt ggcgataatc tcgaggcgga tttaggccaa
    34261 gtctataaca ttaccgccaa tctctctgtg atctcttttg acgacgcaat caaaattggt
    34321 cgtatcgtgc gtgagcaggt acaggtcggt cgggtcatta catttggtgg cctgttaacc
    34381 gacagtcaac gcattctcga tgccgcagaa agcaaagaag ggcgctttat tggtatcaat
    34441 gcgccgcgtt ctggcgctta tgacaacggc ttccaggtcg tgcatatggg ctacggtgtc
    34501 gataaaaaag tgcaggtgcc gcaaaaactg tatgaggcgg gcgtgccaac cgtgctggtg
    34561 ggtaaggtgg cagatatcgt cagcaatcct tatggcgtga gctggcaaaa cctggtggat
    34621 agccagcgga ttatggatat caccctcgac gaatttaaca cccatccgac ggcgtttatt
    34681 tgcaccaata tccaggaaac cgacctcgct ggtcatgcag aagacgtcgc acgttatgcc
    34741 gaacgtttgc aggtcgttga ccgtaacctt gcccggcttg ttgatgcgat gcagccagat
    34801 gattgcctgg tcgtgatggc ggatcacggc aacgatccga ccattggtca cagccaccat
    34861 acccgcgaag tggtgccgct gctggtttat cagcaaggcc tggctgctac gcagctcggt
    34921 gtgcgcacca cgctttctga tgtgggagct accgtgtgtg aatttttccg tgcgccaccg
    34981 ccacaaaatg gtcgctcttt tctttcctcc ctccggtttg caggagacac cctatgagtt
    35041 ttgatccgac gggttacacc ctggcccatg agcatctgca tattgatctc tccggcttta
    35101 aaaacaacgt ggactgccgc cttgatcagt atgcgttcat ttgccaggag atgaacgacc
    35161 tgatgacccg gggtgtacgt aatgtgattg agatgaccaa ccgttacatg gggcgcaatg
    35221 cgcaatttat gctcggtgta atgcgcgaaa ccgggatcaa cgtggtggcc tgtactggtt
    35281 attaccagga tgcgtttttc ccggaacatg tggtgacccg cagcgtgcag gaactggcgc
    35341 aggagatggt cgatgaaatt gaacagggta tcgatggcac ggagctgaaa gccgggatca
    35401 tcgcggagat cggctccagc gaaggaaaga ttacgccgct ggaagagaag gtatttattg
    35461 ccgctgcgct ggcgcataac cagaccggac gcccgatctc cacgcatacg tcgttcagca
    35521 ccatggggct ggaacaactg gctctgttgc aagcccacgg agttgatctt tcgcgcgtca
    35581 ccgttggtca ctgcgatctg aaagacaacc tcgacaacat tttgaagatg atcgatctcg
    35641 gcgcgtacgt gcagttcgac accattggta agaacagtta ctacccggac gaaaagcgca
    35701 ttgcgatgct ccacgcgcta cgtgaccgtg ggttgctgaa ccgcgtcatg ctgtcgatgg
    35761 atattacgcg ccgctcccat ttaaaagcca atggtggtta tggctatgac tatttattga
    35821 ccacctttat tccgcaattg cgccagtcag gattcagtca ggccgatgtg gatgtgatgt
    35881 tacgtgaaac tccctctcaa tttttccaat aaggacagac tcatgaaaaa gattggcgtt
    35941 gcaggcttac agcgtgagca gattaaaaaa accattgaag cgacggctcc tggcagtttt
    36001 gaagttttca ttcacaacga catggaagcg gcaatgaaag tgaaatccgg gcaactggat
    36061 tactacatcg gtgcgtgtaa taccggtgcg ggcgcggcat tgtcgattgc catcgcggtg
    36121 attggctata acaaaagttg caccattgcc aaaccaggca ttaaagcgaa agacgagcat
    36181 atcgccaaaa tgatcgctga aggaaaagtg gcgtttggcc tttccgttga gcacgtcgaa
    36241 cacgcgattc cgatgctgat taaccatctg aaataaaagg cacgactatg gatctgtata
    36301 ttcagattat cgtggtggcg tgcctgacgg gtatgacatc gcttctggcg catcgctcgg
    36361 cggctgtttt tcatgacggt atccgcccga tcctgccgca actgattgaa ggctatatga
    36421 accgtcgcga ggcggggagt atcgcttttg gtctgagcat tggttttgtg gcctcggtgg
    36481 ggatctcttt taccctgaaa accgggctgc tcaacgcatg gttactcttt cttcctaccg
    36541 atatcctcgg cgtactggcg ataaacagcc tgatggcgtt tggtcttggc gctatctggg
    36601 gcgtgttgat ccttacttgc ctgttgccag taaaccagct gctgaccgcg ctgccggtgg
    36661 atgtattagg tagcctcggg gaattaagct cgccggtggt ttctgctttt gcactgttcc
    36721 cgctggtggc gattttctac cagtttggct ggaagcaaag tctggtcgcc gccgtggttg
    36781 ttctgatgac ccgtgtggta gtcgtgcgct atttcccaca tcttaaccct gaatccatcg
    36841 aaatctttat tggcatggtg atgctgctgg ggatcgcgat aactcacgac ctgcgtcatc
    36901 gtgatgaaaa tgatattgat gccagcgggc tttcggtgtt tgaagaacgc acgtcgcgga
    36961 ttatcaaaaa cttaccctat atcgccatcg tgggagcatt gattgccgcc gttgccagca
    37021 tgaagatttt cgccggcagt gaagtgtcga tcttcactct ggagaaagcg tactccgcag
    37081 gcgtaacgcc ggaacaatcg caaacgctga tcaaccaggc ggcactggca gaatttatgc
    37141 gcgggctggg ttttgtaccg ttgattgcca ccaccgcctt agcaacgggt gtgtatgcag
    37201 ttgcgggctt tacctttgtt tatgcggtgg gctatctctc gccgaatccg atagttgcag
    37261 cggtattagg cgcagtggtt atttcggcgg aagtcttgct gcttcgttcg atcggcaaat
    37321 ggctgggacg ctacccgtcg gtgcgtaatg cgtcggataa catccgtaac gccatgaata
    37381 tgctgatgga agtggcgctg ctggtcggtt cgattttcgc agcaattaag atggcgggtt
    37441 ataccggatt ctctatcgcg gttgccattt acttcctcaa cgaatccctg ggccgtccgg
    37501 tacagaaaat ggcggcaccg gtcgtggcag taatgatcac cggtattctg ctgaatgttc
    37561 tttactggct tggcctgttc gttccggctt aaggaaggca cctatgaaga cgtttcctct
    37621 gcaaagtctg acgattattg aggcgcagca aaagcagttt gcgctggtgg atagcatttg
    37681 tcgccatttc cccggcagcg agtttcttac tggcggtgat ttaggcttaa cgccaggact
    37741 gaatcaaccg cgcgttaccc agcgtgtgga acaggtgctg gctgatgcat ttcacgcaca
    37801 ggctgcggcg ctggtgcagg gtgcgggtac tggcgcgatt cgtgcggcgt tggcggcttt
    37861 gctcaaaccg gggcagcgtc ttctggtaca tgatgcgcct gtttacccga cgacacaggt
    37921 cattattgag cagatggggc tgacgcttat tactgctgat ttcaatgacc tgtcggcact
    37981 gaagcaggtc gtcgacgagc aactaccgga tgcggcgctg gtgcagcata cgcgccagca
    38041 gccgcaggac ggctacattc tggcagatgt gctggcaaca ttgcgctcgg ctggcgttcc
    38101 tgcattaacc gatgacaact atgcggtgat gaaagtggcg cgaattggct gcgaatgcgg
    38161 ggcaaatgtc tcgacttttt cctgcttcaa gctgtttggg ccggagggtg ttggcgcggt
    38221 ggtaggcgat gccgatgtta ttagtcggat tcgctccacg ctctattccg gcggcagcca
    38281 gattcagggc gctcaggcgc tggaagtctt gtgtgggctg gtgtttgcgc cagtgatgca
    38341 cgcggtgcag gcgggggtat ctgaacggtt gctggctttg cttaacggtg gtgcggtgcc
    38401 ggaagtgaaa agcgcggtga ttgctaatgc gcagtcgaag gtgttgattg tcgagtttca
    38461 tcagccgatt gccgccagag tgctggaaga ggcgcaaaag cgcggtgcct tgccttaccc
    38521 ggtgggtgca gagtcgaaat atgaaatccc gccgctcttt tatcgccttt ccggaacgtt
    38581 tcgccaggcg aatccacaat cagaacattg tgcgattcgc attaacccga atcgcaacgg
    38641 tgaagagacg gtgctgcgga ttttgcgtga gagtattgcc agtatttaat gctggatagc
    38701 accgtcaagc taaattccgt actgaacggt cccctcgccc ctttggggag agggttaggg
    38761 tgaggggaac gtacgggatg taaaattcaa cgcgccagca tcgccggatg cgatgctggc
    38821 gcatcttatc cggcctacgg ttaatgtctt ttattatcaa tcgttaaggt gatgcgattg
    38881 gcgcggcaga agagttcgga gacgtgcacc ggtttgtcat tctgatcaaa ggcgattttg
    38941 ctgatgcgaa acaacggttc gcccagttcg catttcagcc attttgcctg cgggcgggtg
    39001 gcggcaaaga tatcgatggt ctttttgtcg ctgaccactc gcgtgtcaaa acgctcctga
    39061 aataactgat aggtggagct tccttcgacg taaatctcat caaagtcagg ataacgcgac
    39121 agcgggatcc atgaactatc aataaacagc ggctctttat cgagatacat cacccggcag
    39181 agatggaaca cttcgctgtt tccggggatg ttcagctttt cgcaaaacgg cgcggcgctg
    39241 acccgttcct gttcgatcac tttctctttc gtcgccttgc cttgtgagac gccaaaatcg
    39301 gtaaaaccac tgacagtaag cagggcgttt tcaactttct ggctttgtac aaaggtgcct
    39361 ttgccctgcc agcggatcag tacgccgtct gccactaagt cgctgatagc tttgcgaata
    39421 gtaatgcggc tgacgttata ttgcgtacaa agctcgtttt cggtagggat ctgttgcccg
    39481 gcctggtaaa ccccctgcgc gatatcatcc agcagccgct ggcggacggt agcgtagagg
    39541 agttgatgag agtagcggtc cgtagctgac ataatcgacc ctgaaataaa gaatgaatta
    39601 cacggattat agggtcgttt tccggcaagt aacagcacgt tggcgcggag atgctcacgc
    39661 caacgttata cctaccaggc accgtgatac tgaatggttt tcgccgcgca cgccgttccc
    39721 tgcgccatcg cctgcggtaa tgtcatcccc gcagaccagc cgcaaaggaa tccggcaatg
    39781 aacgaatctc cggcacccat ggtgtcgata accgtcaccg gttcaggagc ctgacgccag
    39841 aactgcgcgc catcccaggc aatgctgccg ttttcaccca gcgtgacaat cactgttcct
    39901 gcgccacggg caacaatcgc tttcatcttc agacacagcg cttcgtcttc ttgcggtgcg
    39961 gaggcaaagg caaaatcgag atgcggcacc agtgtctgcc agagcgggct gtcccacttg
    40021 tcggagaagt cgaaagcggt aagtttgccc gcagcgtgca gctgtgggaa tgcgtcttcc
    40081 gcatgtcccc agattgccgc gtgcacaatg tcatactgcg ccagccaggc gtaatcctct
    40141 tcactcaggg caaagtcggc catcacgcct tcggtgtagt cgccaaaaac gcgatcattg
    40201 tcgtgcagtt ccacctgagt ttgtgcggta acgccgtgtt tcgtatggac atggctgata
    40261 tcgacgccca tgcgggcgag atcctgcttc agctttgtgc cgtagtcatc gtcacccacc
    40321 aaggtaatgc atcccggctg tatgccgtag cgagtgcagt acaccgccac attgaccgca
    40381 ttaccgccag aaaacgcttt attcagttgc gggtagatat cgacgcagtt atcgccgatt
    40441 gtcgccaggg ttttcatctc tcatcctccg gcagcagcgc gcgaaagcgt tccagcgcct
    40501 ggcgggcata gagtctgggc tcgttcatat acatcgtcac cagctccacc gtacagtaac
    40561 cctcatagcc ccgctcaata atatcgcgca tcagttcccg cagcggcatt ttgccttctc
    40621 caggaatgta atgcgtgtcg ctggccccgt cgctgtcgac aatatgcaga tgacgataat
    40681 ttatcgccca gtttgtcgaa ataactcatc accggttccg cctggacata cggcgcgcaa
    40741 atgtcgacca tgctgaacag gcgcggcgaa ggcaccagcg ccagcgcatg aagcacatca
    40801 ttagcattac acacgacgtt cgattcatac ggcgttaacg gttcgaggat tagatccatg
    40861 ccgatgtttt ccgcgtactc acacagttcg cttaggttct ctgccagccg tccccagata
    40921 acattaggtg gcgtgagata gcctgcgtgg gccgcggaaa tcagcgtata acccgcgttc
    40981 atctcttttg ccatatccat cgccagcttg atcatgtcga ggctttcgcg acgcatatgt
    41041 tcatcgccca gcatcatgtt atacggatag ccgttggttt ctggcgtata gccgataatc
    41101 ggcatctgat acgtctgcgc cagcgccttg atttgtttga tgccgcccgc ttttaagtcc
    41161 ggcgcgaacg cgtgcgggcg accgccccaa atttcgatgc cgtcgtaacc cagctcgctt
    41221 gcatcacgaa atgcgtgttc aataggcagc cgctggtggc cgcaggtaaa catacctgtt
    41281 ttcatcgggt aactccttgc gtaaatccgg cccgttgccg ggccgggaga gggattaata
    41341 ttccaccaga ccaccgtaat agcggcgttc atccgggttg tgatctttgt aaatagacag
    41401 gtagtagcag agccactcca ttggcacgaa catcaggaac ggtgccagcc acgggtgcag
    41461 cccttgcgaa atttcggcgt aatcgatgac gatcacgttg tcagtacgct gtttaacaaa
    41521 gttaatggcg cgttcggtgg tgtggcgact ttcatcattg ccgagcagga acaggaacgg
    41581 aacgcccggt tcgacaatct ccagcgggcc atggcggaac tctccgctct caatcacgca
    41641 gccgtgcgtc caggtaaatt ccatcagcgt tacaatgcct tctttgtaac ccagcggacg
    41701 cagcggaccc gcagcaacgg tataaatcat cggccactgg ctggccagtt caccaagctg
    41761 gcggcctttt tcttcccagg tgcgtaccag atgaccgagc gcattcggca acggcttgag
    41821 atcgtttttg atcttgccga tttccgcgtt cggcgcgagg cgggtgatca tctccagcac
    41881 cacgctgtag cagagcagca ggtgaatttc ccagatacag tcggcctgat aatcaatgct
    41941 aaattccgcc gccgaggtaa tcgggctgtc cgcgcgtttg gtgaacgccg cagtgagtgc
    42001 gccgcaggcc cggcccagct ccagcgcttt gattacctct tccgttttac cgtagtcaga
    42061 aacgccaatc accgcgcaac gatcgtcgag gcgatacggg gtgttatcgc agaactccca
    42121 gccggaaatg gcgtagacct gaagatcgga aaagcgatcc gccagatgtt tcgccgtttg
    42181 cgccgcgttg agtggcgagc cgcaggcaac gaaataaata cgatcaatgt cgcgcttcac
    42241 catctcttcc acgatggcgt gcaccagcgg aacgtcatgg ctgagaactt tttccacttc
    42301 ctgaaccata ttttcggtga ccagaaagtc cacggtgctt ttatcaatat ccaacatttt
    42361 attttctcca gataacatta cgacaaagcg ttgagctggc ggctacgctt cgcccagaag
    42421 gcgtaagcag gcagaccagt agcaataacg atgacggcgc agataaggcc gggaatcggt
    42481 gcccagacaa aggttgaggc gaccagaatg aggcttgacg caatggcgag ggtggtcatc
    42541 agcccgaaag ccggagtacg ccacagcggt ttgtaatcgt cgcgtttacg acaccagatg
    42601 atggagccga aggtgagggt atttttgaaa cacatcacca gggtgaaata acccagcagg
    42661 ctggtgagat cggaaacgaa gatgaagaag atccccagcg ccccttgcag gatgatggag
    42721 acatccggcg tgttgtattt cggatgcaca tggccgaagc atttaaagaa caggttgtct
    42781 ttcgccatcg cgtattccag ccgcggctgg tacatcacgc agctggaaag cgaaccaaga
    42841 atgacgatca tcgccgtgat ggcaacaaag atcccagcgg tgctgccgag tgcggggatc
    42901 caggtcaggg cgtcggaaat cggcgtttca gaattggcga gtttgtcgaa gggcatcagg
    42961 ccggaaatca ccagcgccag cagggtgtag agcaccagaa ccagcagaca ggaaccaatc
    43021 agcgctcgtg gcatggtttt tccggggttt ttaatttcgc cggtcatata acagatagag
    43081 gccatgccgg tatacgacca actggtggca gagatccccg ccagcagcgc cataaagctg
    43141 cccgttgcgc caatcgcagt ggtggtaggg gcggcaaaat tctccgcttt aaaccagaag
    43201 atcccaaggc caatgacgat agtgaacggg ataattttgg cgatggtaat tagcgtctga
    43261 aacgctgcgc cgccttcaac ggagcgcagg tgtagcaaca taaaggcgat aattaatccg
    43321 gcggcgataa atttaccgag caacggatcg ataggcgtta aaaagccaag attgctgaca
    43381 atcgccagcg ccataatcga caatgacggt gcatcgttgg cccagaagct ggcccagccg
    43441 gagaggaaag ccagcggtcg gcttccggca tttttcagat aaacataatc tgcgccattt
    43501 tccggataag cggtggatag ttccgcatag acgcacattt gcgggatcac aattaacccg
    43561 ccaatgacaa acgcgagcac cgtaagccac ggcgtgcccg ctgcttttgc cacttcaccc
    43621 acagatacaa aaataccgga gccgacggtt gtcccgacgg cgattgcaag aacggcccaa
    43681 aatccgagct tgcgttggag ttcctggctt cccatagcga tacctttttc ttgtccaata
    43741 cgttgtaggg gagagtttgc agtcatttaa aaggtaacat gacaatgcat gatgaatata
    43801 acaacatatg atgttatgta atgtggagga agatcacaaa atttcgcaca ggatcgcgct
    43861 gtggctaatg gatgtagtta tcaaattgaa tttaaagtga aaatattttt acgggcgggg
    43921 gcaagaagga catagaaaca aatacgccct cggaaaatcc agagggcgtc gggcaattaa
    43981 accggtgtta gccgatttct gtcagagact tactgtgcag taggaccgca accgccaatg
    44041 attttagaaa tagaaattgc cgggtgcagc aggtagtcgt aagagcagtt cttatcacgg
    44101 ttttcaatat gaccagtaca tgcagttaac gtagccagta ctgcacctac cagtgcaact
    44161 ttaataaatt tgttcattta cattccctat gtattatata tta
//
LOCUS       DAZPLI010000018        42740 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZPLI010000018 DAZPLI010000000
VERSION     DAZPLI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42740
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.018"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            198..1418
                     /locus_tag="ACHUU3_000933"
     CDS             198..1418
                     /locus_tag="ACHUU3_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR2081084.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKNSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPV
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1391..2020
                     /locus_tag="ACHUU3_000934"
     CDS             1391..2020
                     /locus_tag="ACHUU3_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR2081085.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVICLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2021..3181)
                     /gene="ybdL"
                     /locus_tag="ACHUU3_000935"
     CDS             complement(2021..3181)
                     /gene="ybdL"
                     /locus_tag="ACHUU3_000935"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR2081086.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3290..4378
                     /gene="hcxA"
                     /locus_tag="ACHUU3_000936"
     CDS             3290..4378
                     /gene="hcxA"
                     /locus_tag="ACHUU3_000936"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR2081087.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4388..4585)
                     /locus_tag="ACHUU3_000937"
     CDS             complement(4388..4585)
                     /locus_tag="ACHUU3_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR2081088.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4767..6872)
                     /gene="cstA"
                     /locus_tag="ACHUU3_000938"
     CDS             complement(4767..6872)
                     /gene="cstA"
                     /locus_tag="ACHUU3_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR2081089.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7053..7466)
                     /gene="entH"
                     /locus_tag="ACHUU3_000939"
     CDS             complement(7053..7466)
                     /gene="entH"
                     /locus_tag="ACHUU3_000939"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR2081090.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7469..8215)
                     /gene="entA"
                     /locus_tag="ACHUU3_000940"
     CDS             complement(7469..8215)
                     /gene="entA"
                     /locus_tag="ACHUU3_000940"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR2081091.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8215..9072)
                     /gene="entB"
                     /locus_tag="ACHUU3_000941"
     CDS             complement(8215..9072)
                     /gene="entB"
                     /locus_tag="ACHUU3_000941"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR2081092.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9086..10696)
                     /gene="entE"
                     /locus_tag="ACHUU3_000942"
     CDS             complement(9086..10696)
                     /gene="entE"
                     /locus_tag="ACHUU3_000942"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR2081093.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10706..11881)
                     /gene="entC"
                     /locus_tag="ACHUU3_000943"
     CDS             complement(10706..11881)
                     /gene="entC"
                     /locus_tag="ACHUU3_000943"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFR2081094.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12256..13221
                     /gene="fepB"
                     /locus_tag="ACHUU3_000944"
     CDS             12256..13221
                     /gene="fepB"
                     /locus_tag="ACHUU3_000944"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR2081095.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(13302..14552)
                     /gene="entS"
                     /locus_tag="ACHUU3_000945"
     CDS             complement(13302..14552)
                     /gene="entS"
                     /locus_tag="ACHUU3_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR2081096.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14663..15667
                     /gene="fepD"
                     /locus_tag="ACHUU3_000946"
     CDS             14663..15667
                     /gene="fepD"
                     /locus_tag="ACHUU3_000946"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR2081097.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15664..16656
                     /gene="fepG"
                     /locus_tag="ACHUU3_000947"
     CDS             15664..16656
                     /gene="fepG"
                     /locus_tag="ACHUU3_000947"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR2081098.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16653..17468
                     /gene="fepC"
                     /locus_tag="ACHUU3_000948"
     CDS             16653..17468
                     /gene="fepC"
                     /locus_tag="ACHUU3_000948"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR2081099.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17465..18598)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUU3_000949"
     CDS             complement(17465..18598)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUU3_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR2081100.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18814..22695)
                     /gene="entF"
                     /locus_tag="ACHUU3_000950"
     CDS             complement(18814..22695)
                     /gene="entF"
                     /locus_tag="ACHUU3_000950"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR2081101.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     TDLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(22692..22910)
                     /gene="ybdZ"
                     /locus_tag="ACHUU3_000951"
     CDS             complement(22692..22910)
                     /gene="ybdZ"
                     /locus_tag="ACHUU3_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR2081102.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(22913..24115)
                     /gene="fes"
                     /locus_tag="ACHUU3_000952"
     CDS             complement(22913..24115)
                     /gene="fes"
                     /locus_tag="ACHUU3_000952"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR2081103.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24358..26598
                     /gene="fepA"
                     /locus_tag="ACHUU3_000953"
     CDS             24358..26598
                     /gene="fepA"
                     /locus_tag="ACHUU3_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR2081104.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26773..27393
                     /gene="entD"
                     /locus_tag="ACHUU3_000954"
     CDS             26773..27393
                     /gene="entD"
                     /locus_tag="ACHUU3_000954"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR2081105.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27515..27667)
                     /gene="hokE"
                     /locus_tag="ACHUU3_000955"
     CDS             complement(27515..27667)
                     /gene="hokE"
                     /locus_tag="ACHUU3_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR2081106.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(28018..28170)
                     /gene="hokE"
                     /locus_tag="ACHUU3_000956"
     CDS             complement(28018..28170)
                     /gene="hokE"
                     /locus_tag="ACHUU3_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR2081107.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            28139..28407
                     /locus_tag="ACHUU3_000957"
                     /pseudo
     CDS             28139..28407
                     /locus_tag="ACHUU3_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28610..29728
                     /locus_tag="ACHUU3_000958"
     CDS             28610..29728
                     /locus_tag="ACHUU3_000958"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR2081108.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            29794..30042
                     /locus_tag="ACHUU3_000959"
                     /pseudo
     CDS             29794..30042
                     /locus_tag="ACHUU3_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            30107..30475
                     /locus_tag="ACHUU3_000960"
     CDS             30107..30475
                     /locus_tag="ACHUU3_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR2081109.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30569..31222
                     /gene="nfsB"
                     /locus_tag="ACHUU3_000961"
     CDS             30569..31222
                     /gene="nfsB"
                     /locus_tag="ACHUU3_000961"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR2081110.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            31330..32577
                     /gene="ybdG"
                     /locus_tag="ACHUU3_000962"
     CDS             31330..32577
                     /gene="ybdG"
                     /locus_tag="ACHUU3_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR2081111.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(32645..34021)
                     /gene="pheP"
                     /locus_tag="ACHUU3_000963"
     CDS             complement(32645..34021)
                     /gene="pheP"
                     /locus_tag="ACHUU3_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR2081112.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(34123..37266)
                     /gene="cusA"
                     /locus_tag="ACHUU3_000964"
     CDS             complement(34123..37266)
                     /gene="cusA"
                     /locus_tag="ACHUU3_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR2081113.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTTLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(37278..38501)
                     /gene="cusB"
                     /locus_tag="ACHUU3_000965"
     CDS             complement(37278..38501)
                     /gene="cusB"
                     /locus_tag="ACHUU3_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFR2081114.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(38517..38849)
                     /gene="cusF"
                     /locus_tag="ACHUU3_000966"
     CDS             complement(38517..38849)
                     /gene="cusF"
                     /locus_tag="ACHUU3_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR2081115.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(38873..40255)
                     /gene="cusC"
                     /locus_tag="ACHUU3_000967"
     CDS             complement(38873..40255)
                     /gene="cusC"
                     /locus_tag="ACHUU3_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR2081116.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            40412..41095
                     /gene="cusR"
                     /locus_tag="ACHUU3_000968"
     CDS             40412..41095
                     /gene="cusR"
                     /locus_tag="ACHUU3_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR2081117.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            41085..42533
                     /gene="cusS"
                     /locus_tag="ACHUU3_000969"
     CDS             41085..42533
                     /gene="cusS"
                     /locus_tag="ACHUU3_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR2081118.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT        10268 a        11795 c        10879 g         9798 t
ORIGIN      
        1 aataatttgg tgttctcccg atttattgac cgtttaaatg agagtttttg atatataact
       61 acagacagct actataactt tcatctattt attcacagcg cgaatgaaaa ataaaacact
      121 ctgctttaca gtgtttactt tataattaac agtcttatct cactgttcac cctgtaacgt
      181 tcagagagcg tcttcagatg tctatttata aaattcctct tccgctcaat attctggaag
      241 ctgcacgaga gcgtattacc tggacactca acaccctacc ccgcgtttgt gtctcatttt
      301 ctggcggcaa aaactctggt ttaatgctgc atctgacagc cgaacttgcc cgacaaatgg
      361 gcaaaaaaat ctgcgttttg tttatcgact gggaggcgca attctcttgc accattaact
      421 atgttcagtc cctgcgcgag ttgtacaccg atgtcatcga agagttttac tgggttgcgc
      481 tcccgcttac gacgcaaaat tccctttcac aataccaacc cgaatggcag tgctgggaac
      541 ctgatgtcga atgggtgcgt cagcccccgc aagatgcgat aaccgatcct gactttttct
      601 gcttttacca gccaggcatg accttcgaac aatttgtacg tgagtttgcc gaatggtttt
      661 cacaaaaacg tccggtggcg atgatgatcg gcatccgtgc ggatgagtcc tacaaccgtt
      721 ttgtcgccat cgccagttta aataaacaac gttttgccga cgataaacca tggactacgg
      781 ccgcaccagg cggtcatagc tggtacattt accccattta cgactggaaa gtggctgata
      841 tctggacctg gtatgcaaat catcaaaatc tctgcaaccc actgtataac ctaatgtatc
      901 aggcaggcgt tcctctgcgt catatgcgaa tttgcgaacc ttttggcccg gagcaacgac
      961 aaggattatg gctctatcac gttatcgaac cggatcgctg ggctgctatg tgcgcacgag
     1021 tcagcggggt aaaaagtggc ggcatttacg ccggacatga taatcatttc tatgggcatc
     1081 ggaaaatcct caagccagaa catttagact ggcaagaata tgcattattg ctgctcaata
     1141 gcatgccgga aaaaacagct gagcattacc gcaataaaat cgccatttat ttgcactggt
     1201 atcagaaaaa aggcatcgag gttccacaaa cccagcaagg ggacattggc gcgaaagata
     1261 tcccctcctg gcggcggata tgcaaagttt tactcaataa cgattactgg tgtcgggcat
     1321 tatcatttag tcctacgaaa gcgaagaact atcagcgtta taacgaacgg ataaaaggaa
     1381 aacgtcagga atgggggata ctatgcaaca acgattaacg caggatttaa cgcaatttct
     1441 cgccagcttg ccggaagatg atcgcatcaa agcgattaat gaaatccgca tggcgatcca
     1501 tcaggttagc ccctttcgtg aagagccagt ggattgcgtt ctgtgggtta aaaacagcca
     1561 acttatgcct aatgattaca acccaaacaa cgtggcacca cccgaaaaaa aactgctgca
     1621 aaaatccatt gaaatagatg gttttaccca acccattgtg gtcacacata cggataaaaa
     1681 tgccatggaa atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct catcatcatt
     1741 aaaattacgc ttaaaaggtt atttgccggt gatctgtctg gaaggtacgc gtaatcaacg
     1801 catcgccgcg actattcgtc ataatcgcgc tcgtggacgc catcaaatca ccgccatgtc
     1861 ggaaatcgtc cgtgaactta gccagttggg atgggacgat aataaaattg gcaaagagct
     1921 gggaatggac agcgacgaag tgctgcgcct gaagcaaatt aacggcctgc aagagctgtt
     1981 tgccgaccgt caatattccc gcgcctggac ggttaaataa ctaaagctgg cgcaggcgtt
     2041 cagctgctgc cagcaacgtc gattccttct tggcaaaaca gagacgaatc agtttatgtg
     2101 ggaagggatc ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc tcctgcgtca
     2161 gccactggca aaactcaaca tcatccaggg tagaaaccgc gctgtaatcc accagcaaaa
     2221 agtatgtacc ttcacacggt aaaatctcca gccggctttc atttaaggca tttaccagaa
     2281 tatcgcgctt ctggcgataa aagtccggta acgcaagata atgctcaggt tctgcacgta
     2341 gcatatcagc aagcgccagc tgtgccgggg tattcaccga aaaggtcaga tactgatgta
     2401 ccttgcgaat ttcggcgctg atgggcgctg gcgcaacaca ataacccact ttccagccgg
     2461 tcatatgata ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc agctgcgaat
     2521 gcgccagcac actggcatgg ccctgttgtg aaaagttgat gtgctcgtag acttcatcgc
     2581 taatgacaaa aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa tcagcctgct
     2641 gccagacagt tgcactggag ttatgcggag tgttgaggat caccagtctg gtgcgctcgc
     2701 ttaataatgc ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc agtgccatac
     2761 gcttcactat tcccccagaa agcgcgatgg cgggggcgta actgtcatag ctgggatcaa
     2821 aacaaatcac ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat aacgcttccg
     2881 tcgcccctgc cgttacggtg atatcgctat cggcatctgg ttgatagcca tacaaacgtt
     2941 ccgttttctg agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc gcgtattggt
     3001 ttgccccctg ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga ccatcaaaat
     3061 caggaaagcc ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg ctcatctggg
     3121 tgaaaatagt ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca
     3181 tttttattgt acctgacgaa gttgcgtgtt gaaggcacta taacacgatg ctagtatttg
     3241 gcaatcaaga cgtttagatg tctaaataaa acaataagga caacacaaca tgcctcacaa
     3301 tcctatccgc gtggtcgtcg gcccggctaa ctacttttca catccaggaa gtttcaatca
     3361 cctgaacgat tttttcactg atgaacaact ttctcgcgcg gtgtggatct acggcaaacg
     3421 cgccattgct gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag gggcaaagca
     3481 tattttgttt cgtggtcatt gcagcgaaag cgatgtacag caactggcgg ctgagtccgg
     3541 tgacgatcgc agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca ccgcgaaagc
     3601 cctcgcccgc cgtctcggtc tgccatttgt tgccgttccg acgatcgccg ctacctgcgc
     3661 cgcctggaca ccgctctccg tctggtataa cgatgccgga caggcgctgc attatgagat
     3721 tttcgacgac gccaatttta tggtgctggt ggaaccagag attatcctca acgcgccgca
     3781 acaatatctg ctggcgggga tcggtgacac gctggcgaaa tggtatgaag cggtggtgct
     3841 ggctccgcaa ccagaaacgt tgccgctaac cgtgcgactg gggatcaata atgcgcaggc
     3901 cattcgcgac gtattgttaa acagtagcga acaggcgctg agcgatcagc aaaatcaaca
     3961 gttaacgcaa tcattttgcg atgtggtgga tgctattatt gctggtggtg ggatggtcgg
     4021 tggtctgggc gatcgtttta cgcgtgtggc ggcggctcat gccgtgcata acggtctgac
     4081 tgtcctgccg caaaccgaga aatttctcca cggcaccaaa gtcgcttatg gcattctggt
     4141 acaaagcgcc ttgctaggtc aggatgatgt gctggcgcaa ttaaccgggg cgtatcagcg
     4201 ttttcatctg ccgaccacgc tggcggagct ggaaatggat atcaataatc atgcggagat
     4261 cgacaaagtg attacccaca ccctgcgtcc ggtggaatac attcattacc tgccagtcac
     4321 gctgacacca gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg
     4381 tgcaaattta gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac
     4441 ggaaaaactc ttcgtaggtc atcggcgttt gatcgggatg gttaacccgc atatgttcga
     4501 catagttgtc gtaatcaggc ataccaatca tcagcttcgc cgcctgacct aaatattttc
     4561 cggcttttgc cagtgaatca aacatcttgg cctccgctaa ccatgttgct gaaagagtgc
     4621 cggatgcggc gtgaacgcct tatccggcct acaaatgcta tcaacgcgct cggtcagtcc
     4681 cctcgcccca tcggggagag ggttagggtg aggaggaacc ctcggcacta tgccaacatt
     4741 cgccaacatc ccccctcact ctgactttag tgtgcgcctt ttgcctgcgc cacgatctcc
     4801 tcgacatttt ccggcattgg ctcatacggc gtttctttcg ccgttggctt cggatctttc
     4861 aatgccgcca gtgccgactt aatcgagaac agcgccagaa ccaccacgac caccataaag
     4921 aagatggtta acccggcatc cagacggttg ttgaacacca gttgcgccag ctgtgactca
     4981 gtatactgcg acggaatatt gccgctgtcg atcattgcct ggaacttatt agcaatggcc
     5041 aggaagccga ctttcgcatc cgggctaaac gctttctgcc agcctgcggt cagggtacaa
     5101 atcagcagcc aggccgttgg taccagcgcc acccaggcgt aacgttgacg tttcatcttg
     5161 aacaacacca cggcacagag catcagcgcc atccctgcca gcatctggtt ggcaatacca
     5221 aacagcggcc acagagtgtt aatgccgccc agcggatcga ccacgccctg atggaggaag
     5281 tagccccacg ccagtacgca cagcgctgtt gccagcaggt tagcaggcag tgaatcggtc
     5341 cgcttcaggc caggagacac cacgcccagc agatcctgca acataaagcg cgcagcacgc
     5401 gtacccgcat ctaccgccgt cagaataaac agagcttcaa acagaatggc gaagtgatac
     5461 cagaacgcca catccatcat accacccagc gcaccgtgca ggatgtaagc catccccacc
     5521 gccagcgtcg gcgcaccgcc cgcacgggaa atgatcgact gttcacccac ttcgctggca
     5581 atctggttga gcgtatccgg agtaatcgca aagccccagc tactcaccac ctgcgcggca
     5641 gaagcgacca catccgccgt ccctgccgga gccagcaccg ccatcgggct gttcatggcg
     5701 aagtacacgc ccggatcgat gatacaggcg gaaaccagtg ccataatcgc cacgaaggat
     5761 tccattaaca ttccaccgta gccgataaag cacgcctgcc cttcgttcgc cagcatcttc
     5821 ggcgtggtgc cggaagagat cagcgcatgg aagcctgaca ccgcgccaca ggcgatggtg
     5881 ataaacagga acgggaacag attaccggtc cataccgggc cagtgccatc gacaaatttg
     5941 gtcagcgcag gcatggtcag cgtcggacgc ataatcaaaa tgcctaccgc cagaccaacg
     6001 atagtcccga ttttcaggaa ggtagagagg tagtcacgcg gggccagcag taaccacacc
     6061 ggcagcaccg ccgccacaaa accgtagccc accagcatcc aggtcagctg cacgccggta
     6121 aagtcaaagt acggtgccca ggtcgggctt tctgccaccc agccgccaga gataatggca
     6181 aaaataagga acaccaggcc aatcactgac acttcgccaa tacgccccgg acgcagatag
     6241 cgcaggtaga ttcccataaa aatcgccagc ggaatggtga acgcaacggt atacgttccc
     6301 cacgggctat gagtcagggc tttcaccacg atcatcgcca gtactgcaag gataatgacc
     6361 atgatcataa agcaggctac cagcgcaatc accccggcag ttggccccat ctcttctttg
     6421 accagctcac ccagcgagcg accgtcacgg cgcgtagaaa caaacagcac catgaaatcc
     6481 tgcaccgcac cggcgagaac caccccggcg agcagccaga tcatccccgg caggtagccc
     6541 atttgcgctg ccagcaccgg ccccaccagc ggccctgctc cggcaatggc cgcaaaatgg
     6601 tgaccgaaca gcactttctt gtccgtcggc acatagtcca gcccgtcgtt atggcgcacc
     6661 gctggcgtca tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata cagcccataa
     6721 aaacggtaag cgatcagata gatacagacc gacgccacca caatccacag cgcgttgatc
     6781 tgttccccac gatttaaagc aatgtatccc agagcaaatg ctcccattac agagagcact
     6841 gtccagacga ggtatttccc tgatttgttc atagttgtta tccgtgtgcg tgtccataga
     6901 gatgttacat tttgtttcta taacatcttt gtacgattta acaacacggt aaccatgtaa
     6961 atcggagttg aaaccaatat ttaacttaac aatgttaact cgatcactcc gttaaccgag
     7021 ttttcctgca atctcattta attctgtcgg gctcatccca aaactgccgt acccagccga
     7081 caagtgcagc aacgccgccc ctgttcatcg aaaacgatga tttcccagct ctgattttgc
     7141 cgcccaagat gcagcggctg gcagacgccg cgtaccttgc cttcagacac cgggcgatga
     7201 tgggtggcgt taagttccgt acccaccaca cactgcccgt cacgggtcat cataaatccg
     7261 gccatcgatc ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag caggccaaac
     7321 ggctgatgag tacgggtatc aaccggcatt tcggcttcca gcacatcatc tcccagacgg
     7381 gtatacacaa ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt cagttcgtcg
     7441 agcgttaaat ggcgtttcca gatcatgctt atgcccccag cgttgagccg ccatcgacca
     7501 caatatcctg tagggtgata tggctggcga ggtcagaggc gaggaacaaa atcgtgttgg
     7561 cgatctcttg tggacgggcg attttcccca gcggaatgcc gagtttaaac tgctcgccaa
     7621 agccgcgaat acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt tgcatatcgg
     7681 tgtcggtgga gccaggcgaa accacattac agcgcacgcc gctaaccgcc agttccagcc
     7741 cgacgctcaa cgccaggctt ttcagcgccg ctttcgatgc gccataagca ctcataccaa
     7801 tacgcggcgt gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc cgctgacggc
     7861 gaaactggtt catggtttgc tgaaacaggt taaacgcacc gccgacgtta accgcaaaag
     7921 tctgctgcca gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa attcccgccg
     7981 cattgaccag cacgtccagt cgctccgttt cagctaacag tcgctgacat acctgcgcga
     8041 cctgcgcagc atcggcaaca tccatcactt cggtcgcaaa gggatattgc tcctgagcga
     8101 acgcttgatc aaaaccggta actttcgctc ccgcttcaac aaacgccagc gccgtggcgt
     8161 agccgatacc tttacctgcg ccggttaccc agacattttt accgctgaaa tccattattt
     8221 cacctcgcgg gagagtagct tccaccaggc gtcgatggtc gggtttttcg ccagcatgac
     8281 aaagtcgata tcgccatgca ctttgcgcca gcgcgccgcc agcgccatca tgcgcaccga
     8341 atccagaccg tagtcgatca ggttgtcgtc atcgaacggt tcatcggact cgtccagcaa
     8401 cggcaggata acctcacgca gcgccgcttt cctggcgggg acaggtgctg gcagtaatcc
     8461 ttcagtcatc accacccggc cagaccgccc ggccacatat ttcagcgaca tcaaatgctc
     8521 gtcacggctg aaatcggcca gcgcatccgc caccataaac ggtttaatat cgcgcataaa
     8581 tgcgtcggtt gcggtggtca tacagccaat gtgggcatat accccggtaa taatcagctg
     8641 gttacgtcca ctctctttga gcatttgctc cagcggagaa cgatgaaacg cgctgtagcg
     8701 ccacttcacc agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca ccttttgctg
     8761 ttccggcgaa cgggtcaggc ccggccccca catatcattc aacagcgccc gatcttcatc
     8821 gctctgctct ttcggctggg cggtgtaata aaccgggata ttgtgctgtt tgcagtagtc
     8881 gcgcagcgca gcaatattcg cgatcacctg ctccatcatc gggcagttct cgccccagaa
     8941 gctgacaaaa tagtcctgca tatcatggat taacaacgcg gcacgttgcg gttcaaaggc
     9001 ccagtcaact ttattctgcg gaatatcgtg agactccggc agtgcgtaag cctgtaattt
     9061 tggaatagcc atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc cactgacgta
     9121 attgtttttt atcgactttc ccgaccgccg tcagcggaag tgaatccaca cactccacgc
     9181 gatccggtaa tttaaattcg gcaataccct gttcacgcag gaaacgacgc acctgcaccg
     9241 cgcgcagcgg ctcttttacc accagataag cacagctttt ttcgcccatc agctcatctt
     9301 ccatgctcac cagtgcggca tagatcaccg ccggatggcg cagcagcagg ttttcgatct
     9361 cttcggcagc gatcttctcg ccgccacggt tgatctgatc tttctcgcgc ccctgcacgg
     9421 tgatgtaacc ctctggatca atagagatca gatcgccgga acagtaaaaa ccgttggcat
     9481 caaaggcact ggcattgtgc tgcggacttt tgtaataacc gcggaaggtg tacggcccgc
     9541 gcgtcatcag gcgtccaact tccccttgcg gcagtggatt tccttcagca tcagcaaccc
     9601 atacttcatc gtccggacac attgggtaac cctgggtatg gataattttc tccgcgctat
     9661 catcaagtcg ggtgtagttc accagccctt ccgccatgcc aaacacctgc tgcaactggc
     9721 agccaatctc agcggaaata cgcgccgcaa gggtggcaga aagacgtgcg ccgccgacct
     9781 gtaacagttt cagcgaggca agctgcgccc ggctttcgcc ttcagccagc gcctgcaacc
     9841 acaggctgac tgccggcggc accagcgcgg taacgttaac ctgatgtttt gcaatcaacg
     9901 ggaagcaaag cgtggcgctg ggatcggccg ccagaacaac cgttcctccg gcaagaaaga
     9961 cgcccagcga tcctggcgaa ctcatagcgt agttatgagc cgccgggatc gcgcacaggt
    10021 agcgtgtctg ttgtgtgaac tgacaaatct cgacgctacg acgcacgctg tagtagtagt
    10081 cgttatgagt gcgcgggatc agcttcggtg taccggtggt gccgccggaa agctggaaat
    10141 aggccacttc atcagcaggt gatggcgtag cagtaaaacc gtctgccgga tggttaatcg
    10201 catcctgcaa gttatgctca ccgctgtcgt tgagcagttg caccacgcga atggaggaat
    10261 gttctgtgac gaaagtattg aggaaatcat ccccgctaaa cagcgcatgt tggcgatcgg
    10321 caatcagtaa cgccggttca atctggctgg cataggcgtt cagttcacta cgctgatggc
    10381 tgaacaacgc cagcaccggc gcaacgccca gtttcagcag tgcgaaaaag gtaatgtaaa
    10441 gttcagcgac gttacccagt tgtaccagcg cggtttcacc gggtttaatg ccctgacggc
    10501 gtaaactaca cgcgaggtta tccgccgcct gattcagctc ccggtaactc aactgtcgct
    10561 cgccgtcgat aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg tcggtcagcg
    10621 gcaaatcctg ccagtagcct ttttcccgat agcgacgggc aaactcttcc ggccagcggg
    10681 tgaatggaat gctcatcctc gctccttaat gcaatccaaa aacgttcaac atggtagaaa
    10741 gtttgacgcc tgtttcgcgc cactcaccca acggtgacga cgcaggcaca atccccgctc
    10801 cggcaaacag acgcacctga ttttcccgca gcttcgcgca gcggatggtc accacccatt
    10861 cgccgttacc ttcgctgtca caccaaccca caatgccgcc aaacagttcg cggtcaaacg
    10921 gctccagttc agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc agcgcggggg
    10981 tcggatgcag cagacaggcc agagtcagtg cgttttcttg cgaattcgct ttaccttcaa
    11041 agggagttgc gagatgccac agcgtcggcg tggtaatcaa ttgtggagag gagggaacgt
    11101 gtaactcact actgcgttcg cgcagtacct ctttcatcgc ctgagtcacc agttcatgtt
    11161 catggcgatc tttttctgac gccagcagac gattacccgc ttcgcgatcg agcacttcat
    11221 ccggctgacg acgcgcggaa ccggctaacg gaatggagct aaaacgctcg ccgtctttac
    11281 gtagcagcag ttccgggctg gcccccagca ggacgccacc atcagccagc gggacatgga
    11341 agttgtaact aaccgggttt tgcgcaatca accgttccag caatacgcca ctatcaatgg
    11401 cggcgtcagt ggtgatatca atcaatcgtg acaacaccac tttgtcgacc tgcggcgtgg
    11461 cggtaagtgc ggcggcgcgg gcaaccatct gttcaaacgt ggtttgttca ggaattgcct
    11521 ggcgttccac cacgttcagc gactggctgc gggtaaaaca gtgggctgag gtctgttttt
    11581 cctgacggga gaacgactgc caggattcgg gaatatacag cgacgaaggc tgacgtggat
    11641 cgaagggaat cgccccgacc atcaccggat ttttgatgcc ctgcgctttg gcatcggcaa
    11701 acagcgcggc gagtttttgc tggaagggac tgtcgggcga atccccgttc acagccggtt
    11761 catcgaagcg ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac ataaagaaaa
    11821 agcgattggg cgcaagtgtt gccatggtct gctgtacttc ctcagccagt gacgtatcca
    11881 tatcatcctc cacaaaatga taaaggcttt aataatgatt atcatttata ttttcggtcg
    11941 ctaacctaaa agcaaacgcg cactatgtca actcttgagg taacgcaatc tgcaactcgg
    12001 ggttgcatct ggttgtggtt acaatgaaaa tgagaagcat tattgatgaa ttcgcataag
    12061 cgcaatgtga tgtcctgcgc cgttttgccc cctctccctt ccagggtgag ggctggggtg
    12121 agggttaatg ttcgcaccag tgctggctgt tcccctcacc ctaaccctct ccccaaaggg
    12181 gcgaggggac ggattgtgcg ctttgtcgaa tttgtcatta cgcccttaac cttattaata
    12241 acaggaagct gacttgtgag actcgccccg ctctaccgca acgcccttct attaacagga
    12301 cttttgcttt caggaatagc cgtagttcag gccgccgact ggccgcgtca gattactgac
    12361 agccggggca ctcataccct ggaaagccag ccgcagcgta ttgtttccac cagcgtcacc
    12421 ctgaccggat cactgctggc gattgatgct ccggtgatcg ccagcggcgc gaccacgccg
    12481 aataaccgcg tcgcagatga ccagggcttt ttacgccagt ggagcaaggt ggcgaaagaa
    12541 cgcaaactgc aacggctcta tatcggcgaa ccgagcgccg aagccgttgc cgcgcaaatg
    12601 ccggatctga ttttaattag cgcaaccggc ggggattcgg cgctggcgct gtatgatcag
    12661 ctttccacca tcgccccgac attaatcatc aattacgacg acaaaagctg gcagtcgctg
    12721 ttaacgcagc ttggcgaaat taccgggcat gagaaacaag cggcagagcg gattgcacag
    12781 tttgataagc aactggcagc ggcgaaagag caaatcaaat taccgccgca gccggtcact
    12841 gccattgtct ataccgccgc tgcacacagt gccaatctct ggacgccgga atcagcacaa
    12901 gggcagatgc tggaacaact cggctttacg ctggcgaagt tgcccgcagg gttaaacgcc
    12961 agccaaagcc agggcaaacg ccatgacatc attcagcttg gtggggaaaa tctggctgct
    13021 gggttaaacg gtgaatcgct attcctgttc gccggtgatc agaaagacgc cgatgcgatt
    13081 tatgccaatc cactgctcgc gcacctgcct gcagtacaaa acaagcaggt ttatacgctg
    13141 ggaaccgaga cgttccgcct ggattactac agcgccatgc aagtgctgga cagacttaat
    13201 tcactgttaa aaaaagattg ataaaacata caattagagc actaataatc ccctcgcccc
    13261 tttggggaga gggttagggt gaggggaaaa tgcaccttta gctaactgtc agacgctgtc
    13321 acctgcggcg gcgtctggcg aaaacgtcgc aactccacca gcaccagcaa taacaacacg
    13381 ccgataatca acaaaccaaa accgctcgcg cttgcggaag caaccggtgt catcatcgcg
    13441 cccaaaccac ccaacagcgc cgcgcctatg gcatcgcccg tcacgttctg cgccgtccac
    13501 aaaccgttaa tccgccctaa catcgcttcc ggggtttgcg tttgcagcat tgtatattgc
    13561 agcaacgaac taaccgcgct cagccagccg aacagcgcca gacaaaccac gcctaaaatc
    13621 cacatcggca tcaggccaaa cagaccaatg gcgaggaacg atcccagcgt ggagagcagc
    13681 atcaataacc ctggtcgcgc actatgtgcc agcttcccgc tggttaacgc accaatagcc
    13741 gcaccgagcg ggatcgccgc atagagaaaa ccaatctgtg ccgccgacat ctgccagttg
    13801 tcagccagcg ccggatacag tacccgcacc gcgctcgcca tcgtcaataa accacccagc
    13861 aacgcaatcc ctcctaccag cgggctggcg agcagaaaac gaaatcctgc cagtaatgat
    13921 ttcaacggat gctcgcgcgg ctgcggtggc ggtggcaacg ccggaaggct taacaacggt
    13981 agcaaggtaa taaacgtgcc cgccgccgcc agcccgtagt tccaggctac gccgccggtc
    14041 gccagcaata aaccgccaat catgggcgaa atcaccgacc ccagacgcac ggtcaacatg
    14101 gtgatcgccc cggcctgcat taagttttca cgccctacca gtgcaggtgt tgccgccagt
    14161 agcgccgtaa caccaagtga tgcgaaaaaa ccatcccata aaccaagtaa atagattgcc
    14221 agcaatgacg gctccggcag cagcgcgttc aggcacagtc caatgaagcc aatgccacag
    14281 gtgccgcgcg ccagcaaaat cacttttttg cgctcatagc gatccgccag cacaccgcca
    14341 accatcaggc caacaaacat cgcgccgccg gtcagcgtca ccgaaagccc cacctgccag
    14401 gtagaatgcg tcatcatctg gatctgcacc ggcaccgcga cgccgagcaa acccagagac
    14461 acaattgaga taaaacgagc gaggaatact gcgcgaaacg ccgggtgcgt tttcaacagg
    14521 ctgaggttaa gcagccagga ttgtttattc attacaatgc cttgccatca aatattttaa
    14581 atacctattc ttaggctgca catgctaaca tatccaaata agatcgataa cgataattaa
    14641 tttcattatc atggaagttc gtatgtctgg ttctgttgcc gtgacacgcg ccattgccgt
    14701 gcccggattg ctgttattac tgattatcgc gacggcatta agcctgctca ttggggcaaa
    14761 atcactcccc gcgtcagtag tgctggaggc cttctcaggc acctgccaga gcgccgactg
    14821 caccatcgtg ctcgacgcgc gactgccgcg cacccttgcc ggtttacttg caggcggtgc
    14881 gcttgggctt gctggggcgt taatgcaaac cctcacccga aatccacttg ccgaccccgg
    14941 cttgcttggc gtgaacgccg gagccagctt tgccattgtg ctgggtgcgg cgctgtttgg
    15001 ttactcatcc gcgcaggaac aactggcgat ggccttcgcc ggggcgctgg tggcctcatt
    15061 gattgttgcc tttatcggca gtcagggcgg cgggcagtta agtccggtgc gtttaaccct
    15121 ggcgggcgtg gcgctggcgg cggtgctgga agggctgacc agcggcatcg ccctgcttaa
    15181 tcctgacgtc tacgatcaac tgcgtttctg gcaagccggt tcgctggata ttcgcaatct
    15241 acatacctta aaagtggtgc tgatcccggt tctgatcgcc ggagcaactg cgctattact
    15301 gagtcgcgcg ctgaacagtt tgagcctcgg cagtgacacc gcgacggcgc tgggcagtcg
    15361 cgtggcgcgc acacagttga ttggtctgct ggcgattacc gtgctttgtg gtagtgcgac
    15421 ggcggtagtt ggcccgattg cctttatcgg cctgatgatg ccgcacatgg cgcgctggct
    15481 ggtaggtgcc gatcatcgct ggtcgctgcc cgtcacgcta cttgctaccc ctgccctgct
    15541 gctgtttgcc gatatcatcg ggcgggtgat tgtgcccggc gaactgcgcg tttctgtggt
    15601 cagtgcgttt attggtgcac cggtgctgat cttcctcgtg cgacgtaaaa cgcgaggtgg
    15661 tgcatgattt acgtctctcg ccgattactc atcacctgtt tgctgctgat ttccgcctgt
    15721 gtggttgcag gtatctgggg attacgcagc ggtgccgtca cgctggaaat ctcgcaggta
    15781 ttcgccgcgt tgatgggcga tgcgccgcgc agtatgacga tggtggtcac cgaatggcgt
    15841 ttaccacgcg tgctgatggc gctgttgatt ggcgcggcgc tgggcgtcag cggcgcgatt
    15901 tttcagtcgc tgatgcgtaa cccgctcggc agccctgacg taatgggctt taacaccggg
    15961 gcgtggagcg gcgtgctggt ggcgatggtg ctgtttggtc aggacctgac ggctatcgcg
    16021 ctggcagcaa tggtgggcgg cattgtcact tctctgttgg tctggctgct cgcctggcgt
    16081 aacggtatcg acacctttcg gttgattatt atcggcatcg gcgttcgcgc catgctggtg
    16141 gcctttaata cttggctgtt gctgaaagcg tctttagaaa cggcgctaac agcgggtttg
    16201 tggaatgccg gatcgctcaa tggcctgacg tgggcaaaaa cttcgccttc cgcacccatc
    16261 atcatattga tgctcattgc cgccgccttg ctggtgcggc gaatgcgctt gctggagatg
    16321 ggcgatgata ccgcctgcgc gctgggcgtc agcgtcgagc gttcgcgtct gttaatgatg
    16381 ctggttgcag tggtgcttac cgctgcggca acggcgcttg ccgggccgat ttcctttatt
    16441 gctttagtcg cgccgcacat tgcccgacgc attagcggca ccgctcgctg ggggctgacc
    16501 caggcggcgc tgtgcggagc gctgttactg ctggcggccg atctctgcgc ccaacaactg
    16561 tttatgccgt atcaacttcc ggttggcgtc gttaccgtca gcctcggcgg tatttacctt
    16621 atcgtcttgt taattcagga gtctcgcaaa aaatgaccga atcagtagcc cgtttgcgcg
    16681 gcgaacagtt aaccctggga tatggcaaat ataccgttgc ggaaaatctg actgtagaaa
    16741 ttcctgatgg tcacttcacg gcaattatcg gaccaaatgg ctgtggtaaa tccacgttac
    16801 tgcgtacctt aagccgcctg atgacgcctg ctcacgggca tgtctggctg gatggcgagc
    16861 acattcaaca ttacgccagt aaagaagttg cacgccggat tggtctgttg gcgcaaaatg
    16921 ctaccacgcc gggcgatatc accgtgcagg agctggtggc gcgtggacgt tatccgcatc
    16981 aaccgctgtt tacccgctgg cgcaaagagg atgaagaagc ggtaacgaaa gcgatgcagg
    17041 ccacgagaat aactcatctg gcagatcaaa gcgtggatac cctttctggc ggacaacgcc
    17101 agcgagcgtg gatcgcgatg gtgctagccc aggaaacggc aattatgctg ctcgacgaac
    17161 ccacgacctg gctggatatc agtcatcaga ttgatctact ggaactgtta agcgaactga
    17221 accgcgagaa aggctatacc ctggcggcgg tgctgcacga tcttaatcag gcctgtcgtt
    17281 acgccagcca tttgattgca ttgcgggaag ggaaaattgt tgctcaggga gcgccgaagg
    17341 agattgtcac tgctgaactg attgagcgca tttatggtct gcgatgcatg atcattgacg
    17401 atccggtagc cggaacgccg cttgtggtgc cgctcggacg aacggcacct tcaaccgcaa
    17461 atagttaaac taagtggtct gccatcatgg catcctgttt tctggatgcc atcgcatagc
    17521 gcaataacac gctaccacaa gccaccatcc cgccgatcag cgcggaaagg atcacaataa
    17581 tcgccttacc cggaccgtct tttttcactg gcaatgacgg gcttaactga tatttaaacg
    17641 gcgtaaaatt cacatcgttg acatttgctt ttgttaattg ctcgacaaga tactgccgat
    17701 tacgtaattc accgttcagt tccgcaacgt cagtaaccgc tttttcaatt tccagtttgc
    17761 gttcaatacc gtctgcgccg agagaaatag agaaatcggg gtcatcttta acggcctgac
    17821 cattactgta tacgggcttt ttaattcctg ccgcgttggc aatgtcgagt gaataattga
    17881 ggcgctgaat gtttgcatca agttgatttt tcgttttaat gcgatcctga gccagttttt
    17941 ctttttcaaa ctgggttttg atctccagtt tattacggac gttttctatc gactctttca
    18001 ccaccaacgc agagatataa tcgatatacc cgctcaaaac ggtctgcgcc tctttactgg
    18061 ttggcgcggt aaaacttagc gtccaggagg tatacagtga cggttcatct tttttcttac
    18121 tggcattgtc atcaaccgct ttcatttttt cgctcaatgc aacaatagcg cgatgcaaat
    18181 ccagttcatc gattttcgcc tcttttaatt ggtccatcac ataaggtgat gaacgcaggt
    18241 actcttccag caagctaaca gactgaaact tcttgataaa caggttaaat gcaccgccac
    18301 ggtcgatatt aacatcgaga tcaagaacgc ggagtttggt aaaggttttc tccaggtcct
    18361 gccactgaat ggcttcggcg ggagtaataa cggctgagct ggtccatttt tgcggcagga
    18421 tgaaagagat cagtaaacct gcgcaggcaa atgcaaaaac gaccgccatg accgtttttt
    18481 tggcccgcca taaaacttcg attagattaa gtaaatcaat ttcattatta ctgggcgacg
    18541 ccagaggata ctcagaaaaa tgagcgtcac ttccctgttt aatattcaat gatgacatgg
    18601 ggtataacct gaaaatgagt ccaatagggg aaaatctcca cggcgctcac tttaccagtt
    18661 ggtgagaatt atccgaagct gaagtttgta aattccttcc actgaatgca atattcactc
    18721 tatttaagag aaatattcat caaacctata aatcaaacaa ataaaaaacc cataattatt
    18781 ttctgtaatt atgggtttat agataatatt aatttacctg tttagcgttg cgcgaataat
    18841 cggcccaatt tttacaaacg cccctggaga gataatatcc acatgcgcac aatcctgacg
    18901 atagatatcc agctccgcaa tccacggcga ccaggcgcgt tcaggactca taccttcctg
    18961 aagcgtgcgt tcagcaacaa acaacgtcgc tttaccgtca aacggtacgc tatgagcagt
    19021 cgtcagcagg cgcacagcat cagcgtagtt gccttcaatg gtggtaaaca actccgttga
    19081 agtacttccc tgctgtgccg ccaggaaggc ctcgcgctcg cggttaatct ccgccagcac
    19141 ttccgggtcc agaccattag cttctttttc ctgccagttt tgcgtttctg gcggccaggt
    19201 atccagcaag ccaagaaatg ccacctgttc gccacgggca cgcagtcgcg ccgcaatacc
    19261 ctgcgccagc gtaccgccaa gggaataccc cagcaggtaa taagggccgt gcggttgttg
    19321 ctcaagtaac gttgccagat gcgcttcgca gacttcatcc aggtttgtcg ctgtctgcat
    19381 gggtccatga gggcgcggcg actgaatgcc aataatcgac cattgtggat cgagataacg
    19441 cgagagtacg ctaaactgcc aggcaaaacc ggacgccgga tggaaacaaa acagcgtcgg
    19501 gccattacct tcacgcaacg gcagaatggt ttcgaatccc atgcgccggg agctgtcctc
    19561 ttcaccatca ataatcgttg ccagtttggc gacggttgac gcgaccatca cctgccccgg
    19621 cgtcacctga cgagcaaact gtcgacttaa ctgcgctgcc agtttcattg ccagtagcga
    19681 atgaccgccc agcgcaaaga aatcagcatc ggcatcctgc acgtcacaac ccagcaacga
    19741 cgcgaatgcc gcggcgataa tcgtttcact gcccgctttc ggtgcacgtc ccgacgcttg
    19801 tgtcttcagt tcaggcaacg gtaaggcttt gcgatccagc ttgccgttgg cgctaagagg
    19861 taactgtgga agttgcagca gaaccaccgg taccatatgt ggtggcaatg tttcacgaag
    19921 ctgcgcctgc aatgcgctgg tatccaacgg caggcccgat tgcgacacca gatagcccac
    19981 caactgacgc gcatcaccac cagtggctgc cgcctggtta atcacacagg cgtgggtaac
    20041 ggcttgttcg acatccggca gcgcctgcat cacgcgatcg atttcgccca gttcgatacg
    20101 ctgcccgcga atttttagct gatcgtcact gcgcccgagg tactccaccg cgccgttatc
    20161 cagccagcgg gcaacgtctc cggtacggta catccgttcg ccaggagcaa aaggatcggc
    20221 aataaagcgg ctggcggtca gatcggggcg tccgagatag ccctgcgcca gttgaatgcc
    20281 ggtaagatag agatcacccg ccacacccgg cggcaccgga tgcatcatcg catcaagaat
    20341 acgcagcccc atgttccaca ccggataacc aatcggcaca ctgctgccgc gcacctgtgc
    20401 cagttcctcg ccaaaagccg gataccagct gacatctacc gccgcttccg tcgggccata
    20461 cagattatgt aacggcgcac cggttaactg ttgccattca cggcataaat cagccggtaa
    20521 ggcttcacca ctacagaaaa cctgtttcaa cgtcgcgcaa ctctggcgag cggtttgcgg
    20581 agtcagcgag gcaacaaatg ccgccagcat cgacggcaca aagtgcgtgg tcgttacgcc
    20641 atattcggca aagaattgct gcatagcgag cgggtcacgg tgcgcttccg gttcagccat
    20701 caccagtttt gcccctgcga taaacggcca gaaaaactcc cacaccgaga catcaaaact
    20761 gcacggcgtt ttttgggcaa cgacatcttc gcctgtaagc gggtagtgat tttgcatcca
    20821 taacaggcgg ttaacgatag ccgtctgccc gaccattacc cctttcggcc tgccggtgga
    20881 gccagaggta aagatgatat aagccgtgtg gtgcggttgt gaaagttgca gcggcgcact
    20941 gccctggggg gtaagcgggg cgttatagca aaggcttgtt aaattgggaa catcgctaaa
    21001 gcgcggcagt tgatcgtcgg tggtaattaa cagcgacgga cgcgcatctt ccagcatcat
    21061 tttcaggcga tcgtccggat agccggtatc cagcggtagc caggccgcgc cagcttcaac
    21121 tattgcatgg agtgccaggg tcaaaaagac cgagcgcggt agtgccaccg ccacgctgtc
    21181 ccctggttta acgccgcgct cacgcagcag attcgccagc gccaccacct gctcgcgcat
    21241 ttcccgatag ctgaacaggt aacgcgcatc tgccagcgcc ggagcatccg gtgtttttgc
    21301 cgcttgttct gccaccagcg cgctcagcgt ggtttctgga atctcaacct gagtggcgtt
    21361 gatctgcgcc agctgcgcat actcacctgg cagcataata tcgacatcgc cgcacaacag
    21421 cgccggatcc gcggcgaact gggcaatcag cattttcagg cgttcagcat gctggattaa
    21481 cgttggctca tcgtaacgct gtttattggc gaggatctca atactcaaat caccgtgttc
    21541 atccgggaac agggccagtt caaggtcatt aaccggaccg gttgccaggg tatgggtttg
    21601 cgcctgaaca ccaggaatat ccagttggta atcaaatacc ttaatattga gtaccggacc
    21661 aaacagcggt tcatcacctg ccgctcgccc gctgtcacgg acaatttgtt cggcatcgta
    21721 acgttgatgg cgacgcattt ttttcagttg agccgccagt cgggttgcca gttccggcag
    21781 cgtttcttgc gccgcaatgt gaatacccaa cggtaaaacg ttgagcacgg gtccgctagc
    21841 cgtcagcgcc gccgagccca gtcgacgcat aaagataaat ccggcggcat agtccatgcg
    21901 attgcacaat cgccccagcc acaaggctgc cagcgcaagg gctaaatcgg tacgctgcac
    21961 acctgaaagt tgcgtagcca gctggcggaa ttccccgtcg gtaaattcca gtttcaggcg
    22021 cagaatatct gccgaagcgc tgcgccccgg taaaggtgcc ggagaaagtg acgcgggcgg
    22081 cggcagttga cgacgctgtt ctgcccagaa tgccgcatca cgctgccagg cttcgctttc
    22141 gcggtattgc tggtactctt ccactacatc agcgaaaggc gtaaacggcg aagcaggcgt
    22201 tggttcgcca cgcagccatg tgcagtaaat attggcgatc tggcgagtaa tggccgggaa
    22261 actgaagcca tcgaccagca aatggtgata acgctgatac cagtaccagc ggttatccgc
    22321 aacatgtatc agctgatgaa agaccagtgg tttaccgcta tcgacgcgca aatcttgttg
    22381 caaatccgtc agcattaatg cctgcgcagt accgtgcgga tcaatattgg ttcgcaggtc
    22441 gataatttct ggctgttcga atatcagcgc atcatcgacc cactgccaga cttcgccgtt
    22501 atcttccgta aaacgcatcc gcaacgtatc tgcttgcgct agtccggcaa ccaccgcgcg
    22561 ggccagtaat ggcgcatcaa catctccagt taactcaacg taatgcgcca cgctccaggc
    22621 ggagggtaaa tccgacagtt tttctgccat ccagatgccg ggctgtgcgg cgaccaaagg
    22681 taaatgctgg ctcattgtgc ctcctgcaac tgggtgaaat tggccggtgt cagagtacgc
    22741 cagtggactt ccagccactg ctggcaggac gcctgtgact gcggctgaca cacaatgtcc
    22801 cagcctgccg ataagaggca ttgttgcggc cacagactga attgcccctg cgcattgcgc
    22861 aaaatgtaaa acgctccctg cggatcatcg aagggattac tgaatgccat actcaactcc
    22921 tgtcatggaa aagtggttgc cagaggtcga ttaacccctg catcaagcca ccgcgccaac
    22981 aaagcgcatc atgtccgccg tcaacctgac gccagaaaat ggattctttt atagggtgca
    23041 attgcgcata cagcgcctga ttggcccgca tgatcatcgg ctcgcgaata cccgcttcca
    23101 gcacaatgcg cagaccttcg gcgctaactt caccagcttt tagcttttca agtaacacgc
    23161 cctcttgctg cccgtcccga tgcggccacc agtacgatcc tgactggctt aatacacagc
    23221 caaagcgttc aggccagtgc agtccggcat acagcgcgga cagcccacca aaactctgcc
    23281 cggcgaccac ggtgcgatcg gcgcgatcgc taaaaggggc aatagctttc accaggggta
    23341 ataactcttg ctgtactgcc agccagaaat ccgcattaca cggcagttcg tgggcgcggt
    23401 gcgtggtgtc gatagcgtcg atcaacacat acacggcggg aggaagttgc tgacgatggg
    23461 tcagcgaagt cagcacaggc cagacgggca tactttgcgc ccaaaattcg ccatcgagca
    23521 aaactgccag cggacgttct tctgctgtta catcgccggt ggtaaaaatc catacacgac
    23581 gtgaattttt caaccgttca cttttccaga taatttcttt ggctgctatt tctggcactt
    23641 gcggacaatc ccatcccggt tgcagaggcg cttgcggcat ttcgagtgca gaaacagcgt
    23701 gccctcgccc gcctttccag ctttgtggat tcagcggatc ggctattgcc tggggtaata
    23761 gttttcgcca gccttcacgt aattcgaggc gatcggggga tggtgcagaa aaaatgtcat
    23821 cgcgttcggt gggaataaag cagtagctgc cgcgccagtt ggcattgagt tgtgtcgtcc
    23881 actgccagac atcagtacct gcaattcgct gcatcgactg gggctggctg ttctgatgat
    23941 gatcggtcac accagtgatg tagatccata cgcgctttat cgtcgagtat tcttcagaac
    24001 cttggggatc acgccaccag aaagtgacct caaacatttc gtcattcaga cgctgccatt
    24061 ccgggccatg tttcgactgc caccagctct cacttcctac ttttaacgcc gtcacaccat
    24121 aaccccatgt ttactgtgca atttttcatt gattgcagaa atatattgat aatattattg
    24181 ataaccattt gcatttgcaa tagcgtaatg gcgcgccgtg ggaagcgcgg acatttatta
    24241 accaactgca ctgcgtgtct ttcaggatca aaggttttcg cggtagcggg atgcgtcgtg
    24301 ttgaggacga ccatgcccga cagttgcaat tcgtggcaaa aatgcaggaa taaaacaatg
    24361 aacaagaaga ttcattccct ggccttgttg gtcaatctgg ggatttatgg ggtagcgcag
    24421 gcacaagagc cgaccgatac tcctgtttca catgacgata ccattgtcgt taccgccgcc
    24481 gagcagaact tacaggcgcc tggcgtttcg accatcaccg cagatgaaat ccgcaaaaac
    24541 cccgttgccc gcgatgtgtc ggagatcatc cgtaccatgc caggcgttaa cctgaccggt
    24601 aactccacca gtggtcagcg tgggaataac cgacagattg atattcgcgg tatgggtccg
    24661 gaaaacacgc tgattttgat tgacggcaag ccggtaagca gccgtaactc ggtgcgtcag
    24721 ggctggcgtg gcgagcgcga tacccgtggt gatacctcct gggtgccacc tgaaatgatt
    24781 gaacgtattg aagttctgcg tggtccggca gctgcgcgtt atggcaacgg cgcggcgggc
    24841 ggcgtggtta acatcattac caaaaaaggc agcggtgagt ggcacggctc ctgggacgct
    24901 tatttcaatg caccagaaca taaagaggaa ggtgccacta aacgcaccaa cttcagcctg
    24961 accggtccgc tgggcgacga attcagcttc cgcttgtatg gcaacctcga caaaacccag
    25021 gctgacgcgt gggatatcaa ccagggccat cagtccgcgc gtgccggaac gtatgccacg
    25081 acgttaccag ccgggcgcga aggggtgatc aacaaagata ttaatggcgt ggtgcgctgg
    25141 gatttcgcgc cattgcaatc gctggaactg gaagcaggtt acagccgcca gggcaacctg
    25201 tatgcgggcg acacccagaa caccaactcc gattcctata cccgctcgaa atatggcgat
    25261 gaaaccaacc gtctgtatcg ccagaactac tcgctgacct ggaacggcgg ctgggataac
    25321 ggagtgacca ccagcaactg ggtgcagtat gaacacaccc gtaactcgcg tattccggaa
    25381 ggtctggcag gcggtaccga agggaaattt aacgaaaaag cgacacagga tttcgtcgat
    25441 atcgatcttg atgacgtgat gctgcacagc gaagttaacc tgccgattga tttcctcgtt
    25501 aaccaaacgc tgacgctggg tacggagtgg aatcagcaac ggatgaagga cttgagttcc
    25561 aatacccagg cgctgaccgg ggcgaacacc ggcggcgcta ttgatggtgt gagtgccacc
    25621 gaccgtagcc cgtattcaaa agcagaaatt ttctcgctgt ttgccgaaaa caacatggag
    25681 ctgactgaca gcaccatcgt aacgccgggg ctgcgtttcg atcatcacag tattgtcggc
    25741 aataactgga gcccggcgct gaacatatcg caaggtttag gcgatgactt cacgctcaaa
    25801 atgggcattg cccgtgctta taaagcaccg agcctgtacc agactaaccc gaactacatt
    25861 ctctacagta aaggtcaggg ctgctatgcc agcgcgggcg gctgctatca gcaaggtaac
    25921 gatgacctga aagcagaaac cagcatcaac aaagagattg gtctggagtt caaacgcgac
    25981 ggctggctgg cgggcgtcac ctggttccgt aacgattatc gcaataagat tgaagcgggc
    26041 tatgtggctg tagggcaaaa cgcagtcggc accgatctct atcagtggga taacgtgccg
    26101 aaagcagtgg ttgaaggtct ggaaggatcg ttaaacgtac cagttagcga aatggtgatg
    26161 tggaccaata acatcactta tatgctgaag agtgaaaaca aaaccacggg cgaccgtttg
    26221 tcgatcatcc cggagtacac gttgaactca acgctgagct ggcaggcgcg ggaagatttg
    26281 tcgatgcaaa cgaccttcac ctggtacggt aagcagcagc cgaaaaaata caactacaaa
    26341 ggtcagccag cggttggccc ggaaaccaaa gaaatcagtc cttacagcat tgtgggtctg
    26401 agcgcgacct gggatgtgac gaagaatgtc agtctgaccg gcggcgtgga caacctgttc
    26461 gacaaacgtt tgtggcgtgc gggtaatgcc cagaccacgg gcgatctggc aggggccaac
    26521 tatatcgcgg gtgccggggc gtatacctat aacgagccgg gacgtacgtg gtacatgagc
    26581 gtaaacactc acttctgatg ctaacgtcag attgttgaca aagtgcgcgt cgttcatgcc
    26641 ggatgcggcg tgaacgcctt atccggccta caaaaatctt gccaattcaa tatattgcag
    26701 gaccatgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgtc atcagtctcg
    26761 aatatggtcg atatgaaaac tacgcatacc tccctcccct ttgccggaca tacgctgcat
    26821 tttgttgagt tcgatccggc gaatttttgt gagcaggatt tactctggct gccgcactac
    26881 gcacaattgc aacacgctgg acgtaaacgt aaaacagagc atttagccgg acggatcgct
    26941 gctgtttatg ccttacggga atatggctat aaatgtgtgc ccgcaatcgg cgagctacgc
    27001 caacctgtct ggcctgcgga ggtatacggc agtattagcc actgtgggac tacggcatta
    27061 gccgtggtat ctcgtcaacc gattggcatt gatatagaag aaattttttc tgtacaaacc
    27121 gcaagagaat tgacagacaa tattattaca ccagcagaac acgagcgact cgcggactgt
    27181 ggtttagcct tttctctggc gctgacactg gcattttccg ccaaagagag cgcatttaag
    27241 gcatcggaga gacaaactga agcaggtttt ctggattatc agataattag ctggaataaa
    27301 cagcaggtca tcattcatcg tgagaatgag atgtttgctg tgcactggca gataaaagaa
    27361 aaaatagtca taactctgtg ccaacacgat taattgacaa catctggtac gattcgcccg
    27421 cagccatcac tgactgcggg cgaaagtgta aagcaggtgc cttaccactc tgacctgaca
    27481 accggatatg cagggattgc tccccgcaca acggctactt cttcggttcg taagcgagaa
    27541 cagccttaaa ctcaatatta cgttccttta cggtgaactc acacagcgag tctccgacca
    27601 gaagcgtaaa tcccagcacc gtcaaacaca gcactatgac tgccacaagg gcatatttcg
    27661 tcagcatgtt tatattgcct ccatgcgaag aagggactac catccgagtt gttgaggttc
    27721 ggagtggcag ccccaggatt gatagaaata tctcctgggg cttcatcttt ccagacctca
    27781 gagtaaacct gaaaccggaa agcttcaggc acccgccgtt atcttaccct tcctcagcaa
    27841 aatatctgtt tatttataca gtaagtcctg tttcggactt ctgttggcaa cactcgttat
    27901 tgctcgtaaa acgaaatcgc ccgcagccat cactgaccgc gggcgaaagt gtaaagcagg
    27961 tgccctacca tcctgacctg acaaccggat atgcggggat tactccccgc acaacggcta
    28021 cttcttcggt tcgtaagcga gaacagcctt aaactcaata ttacgttcct tcaccgtaaa
    28081 ctcacacagc gagtctccga ccagaagcgt aaatcccagc accgtcaaac acagcactat
    28141 gactgccgca agggcatatt tcgtcagcat gtttatattg cctccatgcg aagaagggac
    28201 taccatccaa attgttcggg tttggagtgg cagccccagg gttgatagaa atatctcctg
    28261 gggcttcatc tttccagacc tcagagtaaa cctgaaaccg gaaagcttca ggcacccgcc
    28321 gttatcttac ccttcctcaa caaaatggct gtttatttat acagtaaatc ctgtttcgga
    28381 cttctcactc ctcatttaat caactgaagc tgagcccgtt ttttctgcat gcctcaatcg
    28441 tcaaaattct tcgccatcag ccagctctgc cataacttgc cacattgaaa aagtagccaa
    28501 attgcttcag tcaccatcat caaaaaaaga aacattccag aacttccgca tctcgcccag
    28561 ccgctccgac ttctatactg aatagaaaac gccaacataa gagaaaccta tgccattacc
    28621 cgattttcat gtttctgaac cttttaccct cggtattgaa ctggaaatgc aggtggttaa
    28681 tccgccgggc tatgacttaa gccaggactc ttcaatgctg attgacgcgg ttaaaaataa
    28741 gatcacggcc ggagaggtaa agcacgatat caccgaaagt atgctggagc tggcgacgga
    28801 tgtttgccgt gatatcaacc aggctgccgg gcaattttca gcgatgcaga aagtcgtatt
    28861 gcaggcagcc gcagaccatc atctggaaat ttgcggcggt ggcacacacc cgtttcagaa
    28921 atggcagcgt caggaggtat gcgataacga gcgctatcaa cgaacgctgg aaaactttgg
    28981 ctatctcatc cagcaggcga ccgtttttgg tcagcatgtc catgttggct gcgccagtgg
    29041 cgatgacgcc atttatttgc tgcacggctt gtcacggttt gtgccgcact ttatcgccct
    29101 ttccgccgcg tcgccatata tgcaaggaac ggatacgcgt tttgcctcct cacgaccgaa
    29161 tattttttcc gcctttcccg ataatggccc gatgccgtgg gtcagtaact ggcaacaatt
    29221 tgaagccctg tttcgctgtt tgagttacac cacgatgatc gacagcatta aagatctgca
    29281 ctgggatatt cgccccagtc ctcattttgg cacggtggag gttcgggtga tggatacccc
    29341 gttaaccctt agccacgcgg taaatatggc gggattaatt caggctaccg cccactggtt
    29401 actgacagaa cgcccgttca aacatcagga gaaagattac ctgctgtata aattcaaccg
    29461 tttccaggcc tgtcgctatg ggctggaagg cgtcattacc gatccgcaca ctggcgatcg
    29521 tcgaccgcta acggaagaca ccttgcgatt gctggaaaaa atcgcccctt ccgcacataa
    29581 aattggtgca tcgagcgcga ttgaggccct gcatcgccag gtcgtcagcg gtctgaatga
    29641 agcgcagctg atgcgcgatt tcgtcgccga tggtggctcg ctgattgggc tggtgaaaaa
    29701 gcattgtgag atctgggccg atgactaagt agggaattgc cttacagaag aatatccctg
    29761 acaatggtgt tttaacttac atttaacaat gttatgaaac accctttaga aaccttgact
    29821 accgcagcag gcattttgct gatggctttc ctctcttgcc tgctgctgcc cgcccccgca
    29881 ctggggctga cgctggcaca aaaactggtg accatgttcc atctgatgga tcttagctag
    29941 ctttacactt tattgttttg tctgtggttt ttagtgctgg gcgctattga gtattttgtt
    30001 ctgcgcttta tctggcgacg ctggttctcg ctggcggatt aaacgtcgat tatcgccgta
    30061 cttcgcggca tactttgctt attctccttc gcctaaagga acgtttatgg ataagcaatc
    30121 actgcacgaa acggcgaaac gcctggccct tgagttaccc tttgtcgagc tttgctggcc
    30181 ttttggcccg gagttcgatg tttttaaaat tggcggcaaa atttttatgc tgtcgtcgga
    30241 gctgcgcggc gtccccttta tcaatctgaa gtccgatcca caaaaatccc tgttaaatca
    30301 gcagatatac ccgagcatta agccagggta tcacatgaat aaaaagcact ggatttcggt
    30361 gtatcccggc gaggaaatct ccgaagcgtt acttcgcgat ctgatcaacg attcgtggaa
    30421 tctggtggtt gatggtctgg ctaaacgcga tcaaaaaaga gtgcgtccag gctaaagcgg
    30481 aaatctatag cgcatttttc tcgcttacca tttctcgttg aaccttgtaa tctgctggca
    30541 cgcaaaatta ctttcacatg gagtctttat ggatatcatt tctgtcgcct taaagcgtca
    30601 ttccactaag gcatttgatg ccagtaaaaa acttaccccg gaacaggccg agcagatcaa
    30661 aactctcctg caatacagcc catccagcac caactcccag ccgtggcatt ttattgttgc
    30721 cagcacggaa gaaggtaaag cgcgtgttgc caaatccgct gccggtaatt atgtgttcaa
    30781 cgaacgtaaa atacttgatg cctcgcacgt cgtggtgttc tgtgcaaaaa ccgcgatgga
    30841 cgatgcctgg ctgaagctgg ttgttgacca ggaagatgct gatggccgct ttgccacgcc
    30901 ggaagcgaaa gccgcgaacg ataaaggtcg caagttcttc gctgatatgc accgtaaaga
    30961 tctgcatgat gatgcagagt ggatggcaaa acaggtttat ctcaacgtcg gtaacttcct
    31021 gctcggcgtg gcggctctgg gtctggacgc ggtacccatc gaaggttttg acgccgccat
    31081 cctcgatgca gaatttggtc tgaaagagaa aggctacacc agtctggtgg ttgttccggt
    31141 aggtcatcac agcgttgaag attttaacgc tacgctgccg aaatctcgtc tgccgcaaaa
    31201 catcacctta accgaagtgt aattctctct tgccgggcat ctgcccggct atttcccctc
    31261 agattctcct gatttgcata accctgtttc agccgtcatc ataggctgct gttgtataaa
    31321 ggagacgtta tgcaggattt aatatcccag gttgaagatt tagcgggtat tgagatcgat
    31381 cacaccacct cgatggtgat gattttcggt attatttttc tgaccgccgt cgtggtgcat
    31441 attattttgc attgggtggt actgcggacc ttcgaaaaac gtgccatcgc cagttcacgg
    31501 ctttggttgc aaatcataac ccagaataaa ctcttccacc gtttagcttt taccctgcag
    31561 gggattatcg tcaatattca ggcggtattc tggctgcaaa aaggcaccga agcggcagat
    31621 attctgacta cctgcgcgca gttgtggatc atgatgtatg cgctgctttc agtcttctcg
    31681 ttgctggatg ttattttgaa tctggcgcag aaattcccgg cggcatctca gttaccgctg
    31741 aaagggatat ttcaggggat taaactgatc ggcgcgatcc tggtcggcat tttgatgatc
    31801 tcgctgctga ttggtcagtc gccagcgatt ctgatcagcg gtcttggtgc aatggctgcc
    31861 gtgctgatgt tggtatttaa agatccgatt cttggtctgg tggccggtat tcagctttcc
    31921 gcgaacgata tgctgaaact gggcgactgg ctggagatgc cgaaatacgg cgcagatggc
    31981 gcggtgatcg atattgggtt aaccaccgtc aaagtgcgta actgggacaa taccattacc
    32041 actattccca cctggtctct ggtttctgac tcctttaaaa actggagcgg gatgtcagca
    32101 tctggcgggc gacgtattaa gcgcagtatc agtattgatg tcaccagtat tcgttttctt
    32161 gatgaagacg aaatgcaacg tctgaataaa gcgcatttgt taaagcctta tttaaccagc
    32221 cgccatcagg aaattaatga gtggaatcgc cagcaaggtt ctacggagtc ggtattaaac
    32281 ctgcgccgaa tgaccaatat tggaaccttt cgtgcctatc tgaacgaata tctgcgtaac
    32341 catccgcgga ttcgtaaaga tatgacctta atggtacgcc aactggctcc aggtgataac
    32401 ggtttaccgc tcgagatcta cgcgtttact aacaccgtgg tgtggctgga atatgaaagc
    32461 attcaggctg atatattcga tcacatattt gcgattgtcg aagagtttgg tctgcgactt
    32521 catcagtcgc caaccggcaa tgatattcgc tctctggcgg gtgcatttaa gcagtaatta
    32581 aaaaaaccgc tctcatcgaa tggatgagag cggacatcgt tttgtcggat gtagcgtgaa
    32641 tgccttattt ccgacgcagc gttttaaatg ccacaaacag gaatacaatc cacaccggca
    32701 gcaggatcgc tgacaagcgc atatcatcca tcgtgcacat cagcagcaaa atcatgccga
    32761 ggaaggcaat gcagagatag tttccgaacg gatagagcag cgccttaaac tgtgtttcac
    32821 gcccctgacg tcgcatcgct gcacgaaaac gcagatgcgc cagacagatc ataatccagt
    32881 tcaacagcag cgttgctacc accagcgcca tcagcagacc aaacgctttt tgcggcagca
    32941 gatagttgat taacaccacc agcgaagtga tcgctccgga aagcatcagc gagttaatcg
    33001 gcacaccgcg acggctgacg cgagtcaaaa acttcggcgc attaccctgc acagaaaggc
    33061 caaacagcat gcggctgtta gagtaaaccc cgctgttata cactgacagc gatgctacca
    33121 gaatgacgaa gttcagcgca gaagctacca cgttgctgtc gagattatgg aaaatcatca
    33181 caaacgggct actgttggat ttcacttcca cccacggata gagcgccagt aaaaccacca
    33241 gtgaaccgat gtaaaacagc aggatgcgat acaccacctg atttaccgct tttggaatgc
    33301 ttttatccgg atcgcgcgct tcagcggcag taatcccaat cagctccaga ccgccgaagg
    33361 agaacataat taccgccagc gacaaaatca gcccattcca gccggtggcg aagaaaccac
    33421 cgtagcgcca gaggttgtcg atactggctt tctcgccgcc gtgaccagaa aacagcagcc
    33481 acaggccaaa gccgatcata ccgatgattg ccagcacttt aatcaacgca aaccagaact
    33541 cggtttcgcc atataagcgc acgttcacca ggttaacggc gttgatgata ataaagaagg
    33601 cggcagccca aatccacgtt ggaacatccg ggaaccagta ctgcatatag atgcccgcag
    33661 cggtcagctc tgccattccc accagcacga acattaccca gtagttccag ccagagagga
    33721 agcccgcaaa cggtccccag tatttatagg caaagtgggc aaatgaaccg gataccggct
    33781 cttcgaccac catctcgcca agctggcgca taatcaggaa agcgatgatc ccggcgacgc
    33841 cgtagcccag caatacagcc ggacccgcca tctgaatcgc cggaccaatg ccaagaaaca
    33901 gaccagtacc aattgcgcca cccagcgcaa tcagttgaat atgacggtta tgtaatccgc
    33961 gatgaagcgt cggctcttga ttcgacgcag tatcttccga tacggttgac gcgtttttca
    34021 cgcctttccc ctgtgtgtct tttttgttga ggggcacctt ttaacattta gtgcccatcg
    34081 tcgcaagaca caatccacac ggttaaacgg ggtatcctgc ttttatttcc gtacccgatg
    34141 tcggtgcagc cacatcagct tatacgccgc cgggataata aacagcgaca gcaaaggtgc
    34201 ggtgatcatg ccgccaatca tcggtgcggc aatccggctc atcacctctg aaccagcccc
    34261 cgttccccac agaatcggca gcagaccggc gataatcacc gccaccgtca tcgctttcgg
    34321 gcgcacgcgc agtaccgcgc cgtgatacaa cgcctcatcc agcttctgct cactgaatgt
    34381 ttgcggatta ttcaacgacg gctcggcctc tatggcgtga cgtaaataca tcagcatcac
    34441 cacgccaaat tcggcggcga ccccggcaag ggcaataaaa ccggtgcccg ttgccacaga
    34501 aagatgaaag cccatccacc acagcaacca gatgccgccc accagcgcaa acggtacgct
    34561 gctgataatc agcaacgctt cgcccacccg acggaatgcc agatacaaca gcacgaagat
    34621 gatcatcagt gtcatcggca ccatgagttt cagcttatgg ttggcgcgct ccagtagctc
    34681 gaactgcccg gagaatgcca cgctggtgcc cggttttaac tgcacttttt cagctatcgc
    34741 tttttgcaaa tcgtgaacca ccgacaccat gtcacgatcg cgggcatcga tataaatcca
    34801 gctcgtcggg cgcgcattct cggttttcag catcgacggt ccggtagaga ctttaacgtc
    34861 ggccacgtct gccagggtga tttgctgctt catcggcgtc aggatcggta gctggcgcag
    34921 tgcctgcggg ctatcgcgcc agctttgcgg ataacgcaga ttaatgggat aacgggcaat
    34981 cccttccacc gtttcgccaa ccatcgcccc gcccaccgca gaagtcacaa acaactgcac
    35041 atccgccacc gtcataccgt aacgcgcggc tttttcacgg ttaatctcaa cgttgatata
    35101 gcgcccacct tccagacgct cggctagagc agaagccacg cttggcaccg ttcgcgctac
    35161 ttcttcaatt tgctcagcca tcgcgtcgat atccgccagc acagtgccgg aaactttaat
    35221 gccgatgggg cttttaatgc cggttgagag catatcgata cggttacgaa ttggcggcac
    35281 ccacagattc gccagccccg gcagccgcac ggtgttatcc agttcctcaa tgattttgtc
    35341 catcgtcatg ccaggccgcc actgatcctg cggcttaagc tggatggtcg tttcaaccat
    35401 ctcgagcgga gcagaatcgg tggcggtttc cgctttcccg gttttgccaa atacccgcgc
    35461 cacttcaggt acgctcataa ttagcttgtc ggttttttgc agcatactcg ccgcctctgc
    35521 tgcggaaatc cccggcagcg tcgatggcat atacaacaag tcgccttcat tgatctgcgg
    35581 taaaaattcc ccgccaactt tattgagcgg ccagagaacc gtcagcaccg aaagcgctgc
    35641 caccaacagc gtggttttcg gccagtgcag tactttcagc aatagaggat gataaacacg
    35701 aatcaaaaag cgattgagcg ggttactgct ttccggcgga attttgccac ggatccagta
    35761 ccccatcagg atagggatca ctacgatcgc cagcagcgcc gcgcccgcca tcgcataggt
    35821 tttagtgaac gccaacgggc caaacagacg cccttcctgc ccttccaggg tgaagatcgg
    35881 gataaacgac aacgtgataa tcagcagact gataaatagc gccggaccca cttcaacaga
    35941 tgcatcggtg atcacctgcc agcgcgtttt attatccagc gtggcgtcag gatgctggtg
    36001 ctgccactct tccagccgtt tatgtgcatt ttcgatcatg acgatagcgg catcgaccat
    36061 cgccccgacg gcaatcgcaa tgccgcccaa cgacataata ttggcattca gtccctggaa
    36121 gtgcatgaca ataaaagcaa tacacaaccc cagcggcaac gaaataatcg ccaccagcgc
    36181 cgagcgcaca tgccagagaa acagcgcaca gactaccgcc accacgataa actcttccag
    36241 caacttgccg ctgaggttgt cgatggcgcg gtcaatgagc tggctgcgat cgtatgttgt
    36301 aactatctcc acgccttccg gcagactact tttcagcgtt tccagtttgt ccttcacgga
    36361 ggcgatcact tctcgggcgt ttttgccgga gcgcaggatc accaccccgc ccgccacttc
    36421 gccttcgcca ttcagttcgg caatgccccg gcgcatctcc gggccaatct ggaccttcgc
    36481 aacatcacgc agataaacgg gcacgccatt ttcactggct tttaaaacga tgtgattaaa
    36541 gtcgtcgagc gtttgcagat agccgctggc gcgcaccata tattccgctt ccgccagttc
    36601 gatcgacgaa ccgcccgctt cctggtttga agcatccagc gcgcttttta cttcggctag
    36661 actgatgcca tattgcgcca ggcgctgggg atcgataacc acctgatact ctttcaccac
    36721 gccgcccacc gacgccactt ccgcaacgtc agggatggtt tttagctcat atttgagaaa
    36781 ccagtcctgc aatgagcgta aatcggccag atcgtgctta ccgctgcgat ccaccagtgc
    36841 atattcatag atccagccaa cacccgtggc atctggtccc agctcggcgc tgactcccgc
    36901 aggcagctta ccctgtacct ggttgaggta ctccagcacc cgcgaacgcg cccagtacgg
    36961 atcggtacca tcttcgaaaa tgacatacac ataagagtct ccaaactgcg agaaaccgcg
    37021 cacagtcttc gcgccaggca ccgacaacgt ggtggtggtt agcggataag tcacctgatt
    37081 ttcaacgatt tgcggtgcct gaccgggata gctggtttta ataatcacct gcacatcgga
    37141 gagatccggc agcgcatcca ctggcgtgtt aatgatggtc caggtgcccc agatgctcag
    37201 aaacaacgcg cccatcagca ccaaaaaacg gttcgccacc gagcgacgaa taatccattc
    37261 aatcattggt tattccctca atgcgcattg gtagcacttt cagagcgcat ccgctccagt
    37321 gcgccagaaa tattggcttc ggaatcaatc aggaacaggc cgctggaaac caccttttca
    37381 ccttccgcca gaccagagtg taacgcggtg acgccttgcg atgcctggaa aacggcaacg
    37441 cgtttcggta caaagcgtcc gtcggcatca acggtaatca cccgctgttc gttgccggta
    37501 tcaatcagcg cctgtgacgg aatgagcagc atcggttcgc tggcggtgtt gagttgcaac
    37561 caggcgttca tgcccggttt tagcgcctcg tcggcgttgt cgacttccag acgcagctgc
    37621 agcgtgcggg tcgcggcatc cacgccaggt agcagcgtcc atttgcggat ggtgagtgtt
    37681 ttatccggtc gcgccggaac ggtgagcgta aactgcgagg catctttcac cagccaggcg
    37741 atagactccg ggatcgcagc agtgacccac accgggtcca taccctgaat tttcgctacc
    37801 acgttatctt tggcgatatt cattcccgcg cgcagatcaa acgcggtgat cacgccatca
    37861 atgggtgctt tgagcgtaaa gcgagtctgg attttttgcg tggcgatcag gcggcgaata
    37921 tccgcctccg gcattcccgc cagtcgcagc cgctcaagaa tgccttcagt ctgggtcgcc
    37981 gtaccgccgg tttcgcgcag cagtaaatac tcactctgcg cttccaccca gtcaggaatg
    38041 gtcaggtcga gaagcggtgt gcccttttgt actttatcgc ccacggtaag cggatacacc
    38101 ttgtcgataa acccggcagc gcgagcctgc acaatggcat actgatactc gttgtaactg
    38161 acattcgccg ggaaactctg ggcaaaagtc agcggtccgc gcgtgacggt agccgttttc
    38221 acccccagat tctgcgtctg agtcgggtca atgcgcacac cagacgcaga actctcttca
    38281 tcggcatatt tcggcaccag atccatatcc ataaacggcg atttacctgg tttatcgaac
    38341 cgcgtattgg ggtacatcgg gtcgtaccag aataagattt tacgttctgc ggtcgacgtt
    38401 ttttctgcgg gtggttcagc ctttgcaaac caggtaaaac ctgccgcaga aataataccg
    38461 cccgcgatca tgctgccgat aataagcgcg atttttttca tctcattaaa cctgggttac
    38521 tggctgactt taatatcctg taataaagaa aggttgccct gctggacaaa attaaacgcc
    38581 actttgtcgc cggttttaat ttcactcatt ttcgtctgcg gggtgatggt aaagcgcata
    38641 gtcatctccg gccagttcac ggcagcaatc ggatcgtgat ggatggtgat ttttttgctt
    38701 tccagatcaa taccctttac cacgccagtg gcgctaataa cctgcggttg tacttcgctc
    38761 atggtttcat gatgatgttc gttagcctgg gcattaaagc caataacggt aaacagactg
    38821 aacattgcga cttgcagtgc ttttttcatt tattctctcc tggagttaaa aattattgct
    38881 gccaaccgcc ccctaaggcg gtatacaaag aaatttcgtt aacctgacgg gcataattca
    38941 ggtcgagtaa agtttgtcgg gttgcaaata aagaacgctc ggcatccagc acttccagat
    39001 aacttaccgc gccgtgctga tataatgccc gcgcccgttg caaagtaatt tgcagcgacg
    39061 ccagataacg ctgctgggcg ctgatttgat cgttcaggct ttgacgtaat gccagcgcat
    39121 ctgccacttc tttaaaggcg ttctggattt tctgttcata attcactacc gactgctgct
    39181 ggcgaatttc ggcgatatcc agattggcct ggttgcgtcc ggcattaaaa atggggatct
    39241 caattttagg aataaaattc cacatcccgc tgctagcgtt aaataatgaa gacagatcgc
    39301 tgctggcggt tgatattccg ctggtcaggc tgatagacgg gaaaaatgcc gcacgcgcag
    39361 caccaatatt agcattagcc gccattaacg cgtgttcagc ttccataata tccgggcgct
    39421 gcaataagat ttgcgacgac aagcccgccg gtaatttaac gctttgcagg ctgccgctgt
    39481 ttactgtctg cgcttgcggc agtttgccgt agcttcccaa taacagttgc aatgcattat
    39541 tcgcctgcgc cagttccccc tgacgtttag cgatgtcact gcgggtactt tctatcaccc
    39601 cgcgagcctg ttccagcgcc agaacattgc tgctaccggt caacagttgt ttttcgacaa
    39661 acgcatatga ctgctgataa ttacgcagcg tttcttcggc tatttgcaat tgcgcatacg
    39721 ccagttgctg attgaaatag ctttgcgcga cattagaaac cagcagaata tgcaccgcgc
    39781 gctgagcttc ctcagtggct aaataatttt gtcgctcggc ttcgctcatg ttctttaagc
    39841 gaccgaaaaa atcgaggtca aagctggcgt taaggccggt cgaaaactcc cgtgtcgtgg
    39901 cggtattccc tttaagatta ccgctccagc tgccgctgcc ctcgccattg agctgtgggt
    39961 agcggtcggc atcggtcaga cgatattgcg cccgtgcctc ctgcactttc agcgtcgcca
    40021 tgcgcaaatc ccggttattc accagcgcct cgctaatcag cgtcttcacc tgattatcaa
    40081 caaaaaaggt gcgccagccc acgttctggt agttatctgc tgcgttaacc aagccgttct
    40141 ggctgagtga gaactgctgc ggcacgggca ttgccgtacg ctgataatcc ggtgccaatg
    40201 aacaaccggt tagcgcaagg gccacacaaa atggcagaag tttacaagga gacataggct
    40261 cataatttct ggtgatttta taccgccaac tttactcgcc aggctctgat tttccggtga
    40321 caggaaaatg acaaaattgt cattttgcca ataagcgatt gccatctgat cccgctactc
    40381 tagaattgcc cgggcaacat gcggaggaaa aatgaaactg ttgattgtcg aagatgaaaa
    40441 gaaaaccgga gaatacttga ccaaagggtt aaccgaagcc ggttttgtgg tcgatttggc
    40501 cgacaacggg ctgaatggct accatctggc gatgaccggt gattatgacc tgataatcct
    40561 cgatattatg ctgccggacg tgaacggctg ggatatcgtg cgcatgttgc gctccgccaa
    40621 taaagggatg ccgattctgt tgcttaccgc gcttggcacc attgaacatc gcgtcaaggg
    40681 gctggagttg ggggccgatg actatctggt gaagccgttc gcttttgctg aactgctggc
    40741 gcgggtgcgc actctcctgc ggcgcggggc ggcggtgatt atcgaaagtc agtttcaggt
    40801 tgccgatctg atggtcgatc tcgtcagccg caaagtcacc cgcagcggca cgcgcatcac
    40861 tctgaccagt aaagagttta ctctgctgga gttttttctc cgccatcagg gcgaagtgct
    40921 gccccgctcg cttatcgcct cgcaggtgtg ggacatgaat ttcgacagcg acactaacgc
    40981 cattgatgtg gcggtgaagc ggctacgcgg caaaatcgac aacgactttg agccgaagct
    41041 gattcagacc gtgcgcggcg tgggctatat gcttgaggtg ccggatggtc agtaagccat
    41101 ttcagcgccc gttttcgctg gcaacccgtc tgaccttttt tatcagcctg gccaccatcg
    41161 cggcgttttt cgcctttgca tggatcatga tccactcagt gaaagtgcat tttgccgagc
    41221 aggatattaa tgatttaaaa gaaattagcg ccacccttga acgggtactt aatcatcctg
    41281 acgagacgca agcccgacgc ttaatgacgc tggaagatat cgtcagtggt tattccaacg
    41341 tgttgatttc cctggcagat agccacggca aaacggtgta tcactccccc ggtgcgccgg
    41401 atatccgcga gtttacgcgt gacgccatac ccgataaaga cgctcagggt ggcgaggtgt
    41461 atctcctttc cggcccgacg attatgatgc caggccacgg tcacgggcat atggaacaca
    41521 gcaactggcg gatgattaac ttgccggttg gcccgttggt ggacggcaaa ccgatttata
    41581 cgctctacat cgcgctttcg atcgattttc atcttcatta cataaatgat ttgatgaata
    41641 aacttattat gaccgcatct gtcatcagca tcctgatcgt ctttatcgtg ctattggcgg
    41701 tacataaagg tcacgcgccg atccgcagcg tcagccgtca aatccagaat atcacctcga
    41761 aagatctcga cgttcgcctt gacccgcaga ccgagcccat tgagctggaa cagctggtac
    41821 tgtcgttcaa ccatatgatc gagcgtattg aggatgtctt tacccgccag tccaatttct
    41881 cagcggatat cgctcacgaa attcgcacgc cgattaccaa tctcatcacg caaacggaaa
    41941 tcgccctcag ccagtctcgc agccagaagg agctggaaga tgtgctctac tctaatctcg
    42001 aagagctgac gcgaatggcg aaaatggtca gcgatatgct gtttctcgct caggccgata
    42061 acaaccagct aatccccgaa aagaaaatgc tcaacctggc ggatgaagtc ggcaaagtgt
    42121 tcgatttttt cgaggcgtta gcggaagatc gcggcgtgga gttgcgattt gttggcgaca
    42181 aatgtcaggt tgcgggcgat ccgctgatgc tgcgtcgggc gttaagcaac ctgctttcta
    42241 atgccctgcg ttatacgcca accagagaga caattgtagt gcgctgtcag acggtcgatc
    42301 accaggtgca agttaccgtc gaaaaccccg gtacgcccat tgcgcccgag cacttaccgc
    42361 gattgtttga ccgtttctac cgcgttgacc cttcccgcca gcgaaaaggc gaaggtagcg
    42421 gcattgggct ggcgatagtg aaatcgattg ttgtcgcgca taaaggcacg gttgcagtaa
    42481 cgtcagatgc gcgggggaca aggtttgtga ttatgctgcc ggagagagag tgattattaa
    42541 gttcaaattc agggcgtggt gcatcacctc ctggtattgt tgatcaacaa attagagaaa
    42601 aacttattaa acatatgaaa tatcgaaggc ttttattttt caaagtttca cgagatgttt
    42661 gtatatgaaa cttagttaaa tctgtgtttc ttccaattgt agatcacaat gccctttcta
    42721 aattttcatc gttataaaca
//
LOCUS       DAZPLI010000019        42132 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZPLI010000019 DAZPLI010000000
VERSION     DAZPLI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42132
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.019"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            448..600
                     /gene="mokC"
                     /locus_tag="ACHUU3_000970"
     CDS             448..600
                     /gene="mokC"
                     /locus_tag="ACHUU3_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR2081119.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(704..1834)
                     /gene="dnaJ"
                     /locus_tag="ACHUU3_000971"
     CDS             complement(704..1834)
                     /gene="dnaJ"
                     /locus_tag="ACHUU3_000971"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR2081120.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1923..3839)
                     /gene="dnaK"
                     /locus_tag="ACHUU3_000972"
     CDS             complement(1923..3839)
                     /gene="dnaK"
                     /locus_tag="ACHUU3_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR2081121.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4216..4620
                     /locus_tag="ACHUU3_000973"
     CDS             4216..4620
                     /locus_tag="ACHUU3_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR2081122.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4646..5359
                     /gene="msyB"
                     /locus_tag="ACHUU3_000974"
     CDS             4646..5359
                     /gene="msyB"
                     /locus_tag="ACHUU3_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR2081123.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            5508..6074
                     /gene="satP"
                     /locus_tag="ACHUU3_000975"
     CDS             5508..6074
                     /gene="satP"
                     /locus_tag="ACHUU3_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR2081124.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6109..6696)
                     /gene="mog"
                     /locus_tag="ACHUU3_000976"
     CDS             complement(6109..6696)
                     /gene="mog"
                     /locus_tag="ACHUU3_000976"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR2081125.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6811..7764)
                     /gene="tal"
                     /locus_tag="ACHUU3_000977"
     CDS             complement(6811..7764)
                     /gene="tal"
                     /locus_tag="ACHUU3_000977"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR2081126.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            8043..9471
                     /locus_tag="ACHUU3_000978"
                     /pseudo
     CDS             8043..9471
                     /locus_tag="ACHUU3_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            9541..10317
                     /gene="yaaA"
                     /locus_tag="ACHUU3_000979"
     CDS             9541..10317
                     /gene="yaaA"
                     /locus_tag="ACHUU3_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR2081127.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10470..10766)
                     /locus_tag="ACHUU3_000980"
     CDS             complement(10470..10766)
                     /locus_tag="ACHUU3_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR2081128.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10980..12266)
                     /gene="thrC"
                     /locus_tag="ACHUU3_000981"
     CDS             complement(10980..12266)
                     /gene="thrC"
                     /locus_tag="ACHUU3_000981"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR2081129.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12267..13199)
                     /gene="thrB"
                     /locus_tag="ACHUU3_000982"
     CDS             complement(12267..13199)
                     /gene="thrB"
                     /locus_tag="ACHUU3_000982"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR2081130.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13201..15663)
                     /gene="thrA"
                     /locus_tag="ACHUU3_000983"
     CDS             complement(13201..15663)
                     /gene="thrA"
                     /locus_tag="ACHUU3_000983"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR2081131.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15690..15809)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15745..15810)
                     /gene="thrL"
                     /locus_tag="ACHUU3_000984"
     CDS             complement(15745..15810)
                     /gene="thrL"
                     /locus_tag="ACHUU3_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR2081132.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(16024..16710)
                     /locus_tag="ACHUU3_000985"
     CDS             complement(16024..16710)
                     /locus_tag="ACHUU3_000985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR2081133.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(17110..17250)
                     /gene="yjjY"
                     /locus_tag="ACHUU3_000986"
     CDS             complement(17110..17250)
                     /gene="yjjY"
                     /locus_tag="ACHUU3_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR2081134.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17346..18062
                     /gene="arcA"
                     /locus_tag="ACHUU3_000987"
     CDS             17346..18062
                     /gene="arcA"
                     /locus_tag="ACHUU3_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR2081135.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(18122..19474)
                     /gene="creD"
                     /locus_tag="ACHUU3_000988"
     CDS             complement(18122..19474)
                     /gene="creD"
                     /locus_tag="ACHUU3_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR2081136.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19532..20956)
                     /gene="creC"
                     /locus_tag="ACHUU3_000989"
     CDS             complement(19532..20956)
                     /gene="creC"
                     /locus_tag="ACHUU3_000989"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR2081137.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20956..21645)
                     /gene="creB"
                     /locus_tag="ACHUU3_000990"
     CDS             complement(20956..21645)
                     /gene="creB"
                     /locus_tag="ACHUU3_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR2081138.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21658..22131)
                     /gene="creA"
                     /locus_tag="ACHUU3_000991"
     CDS             complement(21658..22131)
                     /gene="creA"
                     /locus_tag="ACHUU3_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR2081139.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22342..23211
                     /gene="robA"
                     /locus_tag="ACHUU3_000992"
     CDS             22342..23211
                     /gene="robA"
                     /locus_tag="ACHUU3_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR2081140.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(23208..23855)
                     /gene="gpmB"
                     /locus_tag="ACHUU3_000993"
     CDS             complement(23208..23855)
                     /gene="gpmB"
                     /locus_tag="ACHUU3_000993"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR2081141.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23907..24428
                     /gene="yjjX"
                     /locus_tag="ACHUU3_000994"
     CDS             23907..24428
                     /gene="yjjX"
                     /locus_tag="ACHUU3_000994"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFR2081142.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(24513..24839)
                     /gene="trpR"
                     /locus_tag="ACHUU3_000995"
     CDS             complement(24513..24839)
                     /gene="trpR"
                     /locus_tag="ACHUU3_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR2081143.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24929..26866)
                     /gene="sltY"
                     /locus_tag="ACHUU3_000996"
     CDS             complement(24929..26866)
                     /gene="sltY"
                     /locus_tag="ACHUU3_000996"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR2081144.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            27077..28744
                     /gene="ettA"
                     /locus_tag="ACHUU3_000997"
     CDS             27077..28744
                     /gene="ettA"
                     /locus_tag="ACHUU3_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR2081145.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28905..29012)
                     /gene="nadS"
                     /locus_tag="ACHUU3_000998"
                     /pseudo
     CDS             complement(28905..29012)
                     /gene="nadS"
                     /locus_tag="ACHUU3_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
     gene            complement(29051..30283)
                     /gene="nadR"
                     /locus_tag="ACHUU3_000999"
     CDS             complement(29051..30283)
                     /gene="nadR"
                     /locus_tag="ACHUU3_000999"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR2081146.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30304..31686)
                     /gene="radA"
                     /locus_tag="ACHUU3_001000"
     CDS             complement(30304..31686)
                     /gene="radA"
                     /locus_tag="ACHUU3_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR2081147.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31735..32703)
                     /gene="serB"
                     /locus_tag="ACHUU3_001001"
     CDS             complement(31735..32703)
                     /gene="serB"
                     /locus_tag="ACHUU3_001001"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR2081148.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32809..33453
                     /locus_tag="ACHUU3_001002"
     CDS             32809..33453
                     /locus_tag="ACHUU3_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR2081149.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33481..34497
                     /gene="lplA"
                     /locus_tag="ACHUU3_001003"
     CDS             33481..34497
                     /gene="lplA"
                     /locus_tag="ACHUU3_001003"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFR2081150.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34529..34792
                     /locus_tag="ACHUU3_001004"
                     /pseudo
     CDS             34529..34792
                     /locus_tag="ACHUU3_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(34953..35672)
                     /gene="deoD"
                     /locus_tag="ACHUU3_001005"
     CDS             complement(34953..35672)
                     /gene="deoD"
                     /locus_tag="ACHUU3_001005"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR2081151.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35752..36975)
                     /gene="deoB"
                     /locus_tag="ACHUU3_001006"
     CDS             complement(35752..36975)
                     /gene="deoB"
                     /locus_tag="ACHUU3_001006"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR2081152.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(37027..38349)
                     /gene="deoA"
                     /locus_tag="ACHUU3_001007"
     CDS             complement(37027..38349)
                     /gene="deoA"
                     /locus_tag="ACHUU3_001007"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR2081153.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(38476..39255)
                     /gene="deoC"
                     /locus_tag="ACHUU3_001008"
     CDS             complement(38476..39255)
                     /gene="deoC"
                     /locus_tag="ACHUU3_001008"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR2081154.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            39513..41063
                     /locus_tag="ACHUU3_001009"
     CDS             39513..41063
                     /locus_tag="ACHUU3_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR2081155.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            41035..41898
                     /locus_tag="ACHUU3_001010"
     CDS             41035..41898
                     /locus_tag="ACHUU3_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR2081156.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT         9946 a        11409 c        10588 g        10189 t
ORIGIN      
        1 acacggaaag tcatatcagc gctaacgggt gaccctaagc cctgataatc atagcttaag
       61 gtcaggattt ctgagggatt aattaggaaa agtaataatt aatttcttgt cagtcggcgt
      121 tatttgttag gacaatattt caaaaataat ccagcttgtc gtcaataatt gcgataaata
      181 gataaattaa agttaaataa ttaaatcatt aaaaaattac gccacaaaaa tgcgaggcgt
      241 cttcaggatt agaatcgata aaacagaatg ggttaaagag agcgatataa tagcggcggg
      301 tgcttgaggc tatctgtctc aggcattagc tgaacggcag atagagaaaa gccccgagtg
      361 atattttact atcaacccga ggccccctat atgctgaaca catgtagagt gcctcttact
      421 gaccgtaagg tcaaggagaa gagagcaatg aagcagcata aggcgatgat tgtcgccctg
      481 atcgtcatct gtatcaccgc cgtagtggcg gcgctggtaa cgagaaaaga cctctgtgag
      541 gttcacatcc gaactggcca gacggaggtt gctgttttca cggcttacga atccgagtaa
      601 gagcaacggc ggggagtgat ccccgccact tttcaggtgc tcgcatatct tcaacgcacc
      661 ctaattttac ccaagcctgc ccacgggcag gcttttgggg aggttagcgg gtcaggtcgt
      721 caaaaaactt cttcacacca tcaaagaagc tctttgagcg cgggctgttg tgctcaccgg
      781 ttgggccacc gaagctttct tgcagctctt gcagcaactg tttctgcttc tcgttcaggc
      841 ctaccggtgt ttcgacgaca acgcggcaca gcaaatcacc ctgtgcgcca ccgcggacag
      901 acttgacgcc tttaccgcgc atacggaaaa gcttaccggt ctgggtttcg ccaggcactt
      961 tcagtttgac gcgaccatca agggtcggta cttcgatttc gccacccagc gccgccatag
     1021 cgaagttgat cgggacttcg caatagaggt tgttgccttc acgctcgaaa atcgggtgct
     1081 gtttaacctg aacctgaacg tacagatcgc ctgccggtgc gccatgctcg cccgcttcac
     1141 cttcgcccgc aagacggatg cggtctccag tgtccacccc tgccgggatt ttaacggaca
     1201 gcgttttgct gcgctcaaca cgaccatgac catgacattt gttgcacgga tctttgatca
     1261 gcgtaccgcg gccctgacag tgtggacagg tctgctgtac agcgaagaat ccctggcgca
     1321 tctgcacctg accagaacca tgacaggtcg gacaggtctg cggctgtgta cctggttttg
     1381 caccgctacc gtggcaaacg tcacactctt ccagagtcgg aatgcggatc tctttggtca
     1441 cgccacgtac agcttcttcg agggtgagct ccatgttata gcgtaaatca gcaccgcgcg
     1501 ccgcacgttg acgaccacgt ccgccgccaa aaatatcgcc gaaaacgtca ccaaaaatat
     1561 cgctgaagtc tgcgccgccg ccaaaaccgc cgccgcccat gccaccttgc tcaaacgcag
     1621 catgaccata ctgatcgtat gccgcacgtt tttgcgagtc ggtcagaact tcataagctt
     1681 ccttgatctc tttaaatttc gcctcggcct ctttgtcacc ctggttacgg tccgggtggt
     1741 atttcatggc caggcgtttg taggcctttt tgatttcacg ctcttccgct gttttggaaa
     1801 cgcctaaaat ctcgtaataa tcttgcttag ccatcttttt taaattgccc ctagatgaat
     1861 gcacgggcgg agaggaaatt ccccttcgcc cgtgtcagta taattacccg tttatagggc
     1921 gattattttt tgtctttgac ttcttcaaat tcagcgtcga caacatcgtc atctttcgcg
     1981 ttgtttgcag aagcatcagc accggcagtc tgctgctggg catgttgctg ctgggcgatt
     2041 tccatcagtt tctgggaaac ctgtgccagt tcctgcattt tcgcttcgat agcggctttg
     2101 tcttcacctt tcagagcagt ttccagtgca gtcagcgcag actcgatagc agttttgtcg
     2161 tcagccggca gtttgtcgcc tgcttcttca acctgcttac gggtgctgtg cagcagatgg
     2221 tcgccctggt tgcgagtctg taccagctct tcaaacttac ggtcagcttc ggcgttagct
     2281 tctgcgtcgc gtaccatttt ctggatttca tcttcgttca gaccagaaga agccttgatg
     2341 gtgatcttct gctctttacc gctgttttta tctttcgcgg aaacgtgcaa gataccgtca
     2401 gcatcgatat cgaaggtaac ttcgatctgc ggcatgccgc gcggtgccgg gttgatacca
     2461 tccaggttga actgacccag agatttgtta tcagctgcac gtttacgttc accctgcagc
     2521 acatggatgg ttaccgcaga ctggttgtct tcagcggtag agaacacctg gctgtgcttg
     2581 gtcgggatag tggtattttt cgcgatcagc gtcgtcatca caccgcccat ggtttcgata
     2641 cccagagaca gcggggtaac gtccagcagc agtacgtctt ttacgtcacc agtcagaaca
     2701 ccaccctgaa cagcagcacc gattgctaca gcttcgtccg ggttaacgtc tttacgcggc
     2761 tctttaccaa agaactcagc aactttcttc tgaaccattg gcatacgagt ctgaccaccc
     2821 acgaggataa cgtcgtcgat atcagatacg gacaggccag cgtcctgcag tgcaactttc
     2881 agcggctcaa tggaacggtt taccagatct tcaaccaggc tttccagttt cgcacgagtc
     2941 actttgatgt tcatgtgttt cggaccggtc gcatctgcag tgatgtacgg caggttaacg
     3001 tcggtctgct gagcggaaga cagttcgatt ttcgcttttt ctgccgcttc tttcaggcgc
     3061 tgcattgcca gcggatcgtt gcgcaggtca atgccctgat ctttcttgaa ttcttcaacc
     3121 aggtagttga tcagacggct gtcgaagtct tcacccccca ggtgggtatc accgttggta
     3181 gccagaactt cgaaggtttt ttcgccgtca acttcgtcga tttcgataat agaaatatcg
     3241 aaagtaccac cacccaggtc ataaaccgcg atagtacggt tgccagtacc tttgtccaga
     3301 ccgtaagcca gcgcagctgc ggtcggttcg ttgatgatac gttttacttc cagaccagcg
     3361 atacggcctg cgtctttggt tgcctgacgc tgagcatcgt taaagtatgc cggtacggtg
     3421 ataacagctt cagttaccgg ttcacccaag taatcttcag cggttttctt catttttttc
     3481 agcacttcag cagaaatctg cggcggtgcc attttctggc ctttaacttc gacccatgcg
     3541 tcgccgttat cagcagcaat aattttgaac ggcatgatgg aaacatcacg ctgtacttct
     3601 tcgtcctgga agcggcggcc aatcaggcgt ttaatcgcaa acagggtgtt ttgcgggttc
     3661 gtcactgcct gacgtttagc cggctgacca accagagttt caccatcctg ggtataggca
     3721 ataatagaag gcgtggtgcg atcgccttcg gcgttctcca gcacgcgagg agtggtgcca
     3781 tccataatcg ctacacaaga gttggtagta cccaggtcga taccaattat tttacccatc
     3841 taaacgtctc cactatatat tcggtcatca tgtggttgtg agtctgtaat aggggcgaaa
     3901 cgtctggttt caactgccca atttcatttt tttgcagact cactcactgc ggttgactac
     3961 taaatggggt cgtaacccac gtcatcaagg gggagatgca aaaatttttt gtgcttttgc
     4021 gcagaaattt cgcgatatgg caggacaaga cgtaaaattg acagcaattt tattcatata
     4081 aagaatgaat cgcagccaag gcattcatca aaaaattgta ataaaaagaa aagattacgt
     4141 acctgaatct tctctttatc agcagtaaac tagtgggtat tcatccctct acctcttccc
     4201 actaagagaa tccttatgaa atccgttttt acgatttccg ccagcctggc gattagcctg
     4261 atgctgtgct gcacggcgca ggcaaacgac cataaactcc tcggcgtcat tgcaatgccg
     4321 cgtaacgaaa ccaacgatct ggcgctgaaa cttcctgttt gtcgcattgt gaaacgcata
     4381 caactctctg ccgaccatgg cgatttacag ttaagcggtg catcggttta tttcaaagcc
     4441 gcccgtagcg ccagtcagag tctgaatatt ccttcagaaa taaaagaagg gcaaaccact
     4501 gactggatca acattaacag tgataacgac aataaacgct gcgtctcaaa aatcaccttt
     4561 tccggtcata cggtgaactc atcggatatg gccacgctga aaattatcgg cgacgactaa
     4621 cgccagaata tagaaaccac aaaaaatgaa tgttaattac ctgaatgatt cagatctgga
     4681 ttttcttcag cattgtagtg aggaacagtt ggcaaatttc gcccgattgc tcacccataa
     4741 tgaaaaaggc aaaactcgcc tctccagcgt actaatgcgc aacgaactgt ttaaatcgat
     4801 ggaagggcat cccgagcaac atcgccgcaa ctggcagctg attgctggag aattacagca
     4861 ttttggtggc gatagtatcg ccaacaaact gcgcggacac ggtaaattgt atcgggccat
     4921 tttgctcgat gtttcaaaac gattgaagct gaaagccgac aaagagatgt ctacgtttga
     4981 gattgagcaa cagttactgg aacaatttct gcgtaacacc tggaagaata tggacgagga
     5041 acataagcag gagtttctgc acgcggtcga tgccagggtg aatgagctgg aagagctgct
     5101 gccgctgctg atgaaagaca aattattggc aaaaggcgtg tcgcatttgc tttccagcca
     5161 attgacccgc attttacgca cccacgcagc aatgagcgtg cttgggcatg gtttgctgcg
     5221 cggcgcgggt ctgggaggcc ctgtgggcgc agcattaaat ggggttaaag cggtcagcgg
     5281 cagtgcctat cgcgtgacaa ttccagccgt aatacaaatc gcctgcctgc gccggatggt
     5341 tagcgccact caggtctgac accgtaaacc ggggaaaatt ttcattttcc cctcaaaaga
     5401 tcgtagacac tgccccactg gctgattatt atgccgcgcc ctgaaaacac tactgttatt
     5461 cagggaaatt atttcaccat tcattcgatg atgatttttg aggaattatg ggcaacacta
     5521 agttggctaa tccggcaccg ctgggcctga tgggcttcgg catgaccacc attctgctta
     5581 acctgcacaa cgtgggttat ttcgctctgg acggtattat tcttgccatg ggcattttct
     5641 acggcggcat cgcgcaaatt tttgccggtc tgctggagta caaaaaaggc aacactttcg
     5701 gtttaaccgc attcacctct tacggttctt tctggttgac gctggttgcg attctgctga
     5761 tgccgaaact gggtctgacc gatgcgccaa atgcacagtt ccttggtgtc tacctgggtc
     5821 tgtggggcgt atttacgctg tttatgttct tcggcacgct gaaaggcgca cgcgttctgc
     5881 aattcgtttt ctttagcctg actgtgctgt ttgccctgct ggcgatcggt aacattgccg
     5941 gtaacgccgc aatcatccac tttgccggct ggattggtct gatctgtggt gccagtgcaa
     6001 tctatctggc gatgggtgaa gtactgaacg agcagtttgg tcgcaccgtt ctgccgattg
     6061 gtgaatccca ctaattgttt tgagatcccc ccgctcgggg ggattttttt attcgctaac
     6121 gtcgcgtctt gcactcttcg gtctgaatgc agcaaccact tctggtgccg tttcaacgta
     6181 tggcccttcc agcaactgaa tgcagtacgg tacgctggca aaaataccgt gtaccacaac
     6241 gttacccgca gcgtccttca caccttccag cgtctcttta atagacttcg gctgaccggg
     6301 taagttaagg atcagcgcct gtttgcgaat cacaccaacc tgacgcgaaa ggatcgcagt
     6361 tggtacaaaa tgcaggctta tctggcgcat ctgttcacca aagccaggca tctcgcggtc
     6421 cgctactgcc agcgtcgcat cgggcgttac gtcacgacgc gccgggccag ttccgcccgt
     6481 ggtgagcacc agatggcaac tcatttcatc cactagctca cacaacgttt gctcaatgat
     6541 cgcctgctca tcggggatta agcgggtttc cagttcaaac ggcgtggtta gcgccgatgt
     6601 cagccattct tccagcgcag ggatgccttt atcctgataa acgccgctgg atgcgcgatc
     6661 agagatggaa actaagccaa tacgtaaagt attcatcaag atttttccgt ttaaacagtc
     6721 tcgttaaaca gaatgataca ctgcgaaggg agtgacagac aggcttcaga agaggtagcg
     6781 tgaccgactt cccggtcacg ctaagaatga ttacagcaga tcgccgatca ttttttccag
     6841 tttttcctgg tcaatagcaa acttacggat accttccgcc agtttatcta ctgccattgg
     6901 atcctggttg tgctgccaca ggaactcgga ctcagtgata cgcgccggac gcgctttcac
     6961 ttcgccggtg taagacagtt tacgttcgat agccccttcg ctctcagcca gctctttcag
     7021 cagtgccggt gcgatggtaa gacggtcgca gcctgccagt tccagaattt cgccgatgtt
     7081 acggaagctt gcgcccataa ccacggtttc ataaccgtgc tctttgtagt actggtagat
     7141 ttcagataca gaaaccacgc ccggatcttc tgccggagcg tactctttct tatcagtatt
     7201 cgctttgtac cagtcaagaa tacggccaac aaatggcgag atcaggaaca cgcctgcttc
     7261 cgcacaagca cgagcctgag cgaaggagaa cagcagggtc aggttacagt tgatgccttc
     7321 tttttccagc tgttctgcag cacggatacc ctgccaggta gaagccagtt tgatcagaat
     7381 acgatcgttg ctaataccag catcgttgta gagtttgatc aggcgttttg ctttcgcaat
     7441 tgacgcttcg gtgtcatagg aaagacgcgc atcaacttca gttgagatac ggcccggaac
     7501 cagtttcagg atttccagac caatatttac tgccagtttg tcggtcgcgt ccacgatctg
     7561 ctgcgcgcga tcgttgctct gctgtttcgc ccaggcgaca gcatcatcaa tcaacttacg
     7621 gtattccgga atctgcgctg cgttaagaat gagagaaggg ttggttgtgg catcctgcgg
     7681 ttgatacagc ttcattgccg cgatgtcccc agtgtcggcc actacggtgg tgtactgacg
     7741 aagggaggtc aatttgtccg tcatgatagt atttctcttt aaacagcttg ttagggggat
     7801 gtaaccggtc tgccctgatg atatcacgac gcaacgtcag cgcaaccgcc gacaagacgg
     7861 ttatcgccag gcgagactgt ttcggatttc tgacactgcg ttgatatcgc ccgccatttt
     7921 tatacaaaac ctcatgtatg ctacgcagaa gttatcaagt acctcgtagc gtatatactt
     7981 cttaaacaat tggtaacgtt tacacaggaa agtcatcgcg accggcaata agagggatat
     8041 gcatgccaga ttttttctcc tttattaaca gcgtcctttg gggatcggta atgatttacc
     8101 tgctcttcgg cgcaggttgt tggttcactt ttcgcaccgg atttgtgcag tttcgctaca
     8161 tccgccagtt tggcaaaagt cttaaaaata gcattcatcc acagccaggc ggtttaacct
     8221 catttcagtc attgtgtacc agtcttgcgg cgcgcgtggg tagcggcaac ctggccggcg
     8281 ttgcgctggc tattaccgcc ggtggacctg gagccgtctt ttggatgtgg gtgccgcgtt
     8341 tatcggcatg gcgacctcgt ttgccgaatg ttcccttgca caactttata aagaacgtga
     8401 cgtcaatgga cagtttcgtg gaggaccggc atggtatatg gcgcgcgggc tggggatgcg
     8461 ttggatgggc gttctgttcg ccgtcttttt gctcatcgcc tacggcataa ttttcagcgg
     8521 agttcaggcg aacgccgttg cccgagccct gagttttctt ttgattttcc cccgctggtg
     8581 acaggcatta ttctcgctgt ctttgctctg ctggcaatca ctcgcggtct tcatggcgtc
     8641 gcccggctca tgcaggggtt tgtcccgttg atggcgataa tctgggtgct gaccagcatg
     8701 gtgatttgcg taataaatat cgggcaactt ccccacgtca tttggtctat ttttgagagt
     8761 gcttttggct ggcaggaagc ggctggcggc gcggcgggat ataccttaag ccaggcgatt
     8821 actaacggtt ttcagcgcag tatgttttcc aatgaggcgg gaatgggttc gacgccaaac
     8881 gcggcagcgg cagcggcgtc ctggcctccg catccggcag cgcaagggat tgtccagatg
     8941 attggcattt ttatcgacac cctggtcatc tgtacggcaa gcgccatgct gatattactg
     9001 gcgggtaacg acacaaccta catgccgctg gaaggtattc agcttatcca gaaggcgatg
     9061 cgggtgctaa tgggttcctg gggtgctgag tttgttaccc tcgtggttat tctgtttgcc
     9121 ttcagctcca tcgttgccaa ctacatttat gccgaaaaca atctcttctt tttacgcctg
     9181 aacaacccta aagcgatctg gtgtttgcgg atctgcacct tcgcaacggt catcggcggc
     9241 accttgctaa gtcttccgct gatgtggcaa ctggcagata tcataatggc ctgcatggct
     9301 attaccaatt tgaccgccat tttactgctc tcgcctgtgg ttcataccat tgccagtgat
     9361 tatctacgcc agcgtaaact cggcgtgcgc ccggtgtttg atccgttgcg ttatccggat
     9421 atcggtcgcc agctttctcc ggacgcgtgg gatgatgttt cgcaggagta atcacaacta
     9481 tcgatcaact cattctcttt ttttgctaaa gtcggcataa atttcctgca aggactggat
     9541 atgctgattc ttatttcacc tgcgaaaacg cttgattacc aaagcccgtt gaccaccacg
     9601 cgctatacgc tgccggagct gttagacaat tcccagcact tgatccatga agcgcggaaa
     9661 ctgacgcctc cgcagattag cacgctgatg cgcatcagcg acaaactggc gggtatcaac
     9721 gccgctcgct ttcatgactg gcagccagat ttcacgccgg agaatgcccg ccaggctatt
     9781 ttggcgttta aaggtgatgt ctacaccggc ttgcaggccg aaactttcag cgaagacgat
     9841 ttcgattttg cccaacagca tttgcgaatg ctttccggct tgtatggcgt actgcgcccg
     9901 ctcgatttaa tgcagcctta tcgtctggaa atggggatcc gtcttgagaa tgcccgaggg
     9961 aaagatctgt atcaattctg gggagatatc atcaccaaca agctgaacga ggcgctcgca
    10021 gcacaaggcg ataatgtggt gattaatctg gcgtcagatg agtattttaa atcggtgaag
    10081 ccgaagaaat tgaatgccga gattatcaag ccggtgttcc tcgatgagaa gaacggcaaa
    10141 tttaagataa tcagcttcta cgctaagaaa gcacgcggtc tgatgagtcg tttcattatt
    10201 gaaaatcggc tgaccaaacc agagcaactg actggtttta atagcgaagg ttacttcttt
    10261 gatgaagctt cctccagcaa tggcgaactg gtgtttaaac gctacgagca gcgttaagtt
    10321 tgcagtcaat gccggatgcg gcgtgaacgc cttatccggc ctacaaaagc atgcagattc
    10381 aatatattgc agaattcacg taggcctgat aagacgcgcc agcgtcgcat caggcgctga
    10441 atgccggatt gttacccggc atttgtcatt tagcggtgat gtttgcctgg accatgaccg
    10501 ccgtgatgat catgaggagc tttcttatgg tggcgcggcg gtggcggcgg tccgtgtggg
    10561 tgccagcgat tgcctcgcca ttcataatgt tgtttccacc agccgtggtc gcgccagtga
    10621 cctccatccc agtaatagcc acgattatca cgatcgccta tctgtaattt tactgacggg
    10681 actaacgtaa tttccgcagc ctgtgctgcc atgggagcga ccagaaccag ggaaagtgcg
    10741 agtacgatag attgcatctt tttcacaggt tactcctttt atcattgccc gttatgggtc
    10801 gatatccgga gattacgcga tgagaacgac ggttgttatt ctccgcaatc ctaatctcta
    10861 agttaccgca tttattgaga atttctcctt tttccctgtc gtttttctcc ataatttctt
    10921 cataaaaaag ccgggctgca taaaagcaaa cccggcctga ttgagataat gaatagattt
    10981 tactgatgat tcatcatcaa tttacgcaac gcagcaaaat cggcgggcag attatgtgaa
    11041 agcaagggta aatcagcacg ttctgccagc tcttttggca gatccaacgt ttcaccgaga
    11101 atcgcttcca cgctctcttt aaatttcgcc ggatgcgcgg tgccgaggaa caagccatat
    11161 tcgcctggat tcaactggtc acacagcgca cgatacgcta cggcagcgtg cggctccgag
    11221 gtgtagccca gttcttttaa ctcacgcatt gtctgttgcg tggtttcatc atccacggct
    11281 gcataaccca gctctttcag ttgccagatt ttgcggcgga acaactcttc cacacgcggc
    11341 cagttgttcg gctgactcac gtccatcgcg ttggataacg tcgcctgagt cgctttgggt
    11401 gaccactgac cgtcgtgcag gaaacgtggc acggtatcgt tcacgttggt cgcagcaata
    11461 aaacgtttca ccggcagacc gagtgacttc gccagcagac ccgccgtcaa atcgccgaag
    11521 tttccgcttg gcaccgagac aaccagctgg ttgcgcgctt cctgcggcag ctgcgcaact
    11581 gcttcaaagt agtagcaaat ctgcgccagc aaacggctga tgttaatcga gttagccgag
    11641 tttaacccca gcgccacttt cagttcttca tcatcaaacg cctgcttcac cagcgcctga
    11701 caggcatcga aatcgccgtc gatggcaaca gtttcgatat tgccgcccaa cgtacagaac
    11761 agtttttctt gcagtggact gattttgcct cgtggataga ggataaccac tttcacattc
    11821 ggcaaaccgt agaaagcatg agccactgcc gctccggtat caccggaggt cgcggtcaga
    11881 atggtcactg gcttatcgcc cgcaatatgg gtcagcattt gtgccataaa gcgaccgccg
    11941 aaatctttaa atgccagcgt tggcccgtgg aacaattcca gacaaccgac atcgctttca
    12001 acattggcga ccggagccgg gaaggcaaac gccgcgcgca cgcgctcttc caggatttcc
    12061 tgcgggattt catcaccaat aaacgccgag aggatcttcg cactgcgggt gacaaaatcc
    12121 agcttcagca tctcatcaat ttcagtcagg ctgaattccg gcaggtcatg cggaaaaaac
    12181 agcccctgat ttttgcccaa cccctgggtt acggcttgcg caaagctgac ctgctcgttg
    12241 tgatctttca gattgtagag tttcatttag ttttccagta ctcgtgcgcc cgccgtatcc
    12301 agccggcaaa tatgaacaaa accttcctga ttttgcaggt agttcttacc caaccagtcg
    12361 gcaacgcgct gggcggtatc cggcttgtca catagagcga acaaggtcgg gccggagccg
    12421 gagataccgc tcgctaccgc gccgatttcc gcgaccgcct gccgcgcctg ccggaagcca
    12481 ggcagtaacc gttcacggta gggttcagcg ataacatctt tcatcagctt cgcggcaagc
    12541 tcaggctgac gggaatagca ggcgtgaatg aagccagcca gatgtcgccc gtgcgcaatg
    12601 caatcctggc ggcgatactg cgccggtaaa atagcccggg cttctgccgt cgagacttta
    12661 attcccggat acgccagcac ccacagccac tcatcaaacc ctggcacttg ctggctgatg
    12721 atgtcgtttt cttcgatcat caactgcata ccaccaagaa aacacggtgc cacgttgtcg
    12781 taatgaatgc tgccggagat acgtccttcc agctcgccca tcaaagccag caaacgggtg
    12841 tcattaagtg gcttgccgca gtgttcattc atcgccatca gcgccgcgac caccgaacag
    12901 gcgctggagc ctaagcccga gccgatcggc atattctttt ccagagtcat cgccactgga
    12961 atttgcttgc ccagctccag gcaaaaacgc tcccagcact gataaacgat attttcccgt
    13021 ggttctgacg gcagcttatc ggcaaagcgt ccgaggttgt tgagactgaa tgtctctgcc
    13081 gcctcaaccg tgactacatc tccgagcaat gcaccatcaa cgggtgtcac cgccgccccg
    13141 agcacatcaa acccgacgct catattggca ctggaagccg gggcataaac tttaaccatg
    13201 tcagactcct aacttccatg agagggtacg tagcagatcg gcaaagacac cagcagcggt
    13261 aacgtcattg cccgcaccat atccgcgcag caccaacggc agcggctgat aatagtggct
    13321 ataaaaggcc agggcgtttt cgccattttt cactttgaac agcggatcat tcccatccac
    13381 ttcggcaatc ttcacgcggc agacaccatc ttcatcaata ttgccaacat agcgcaaaac
    13441 ttttccttca tcacgggcct tcgccacgcg cgcggcaaag agatcgtcga gctgtgacag
    13501 attcgccata aaagcggcaa catcaccctc ggcgttaaac tctgcgggca gcacaggttc
    13561 aatttcaata tccgccagct ccagttcacg tcccgtttca cgagcgagaa tcaatagttt
    13621 acgcgccaca tccataccag aaagatcatc tcgcgggtcc ggttcggtat aacccatttc
    13681 ccgcgccagc gtggtcgcct cggagaaact catgccttcg tctaacttgc cgaagatata
    13741 agaaagcgaa cctgaaagaa tgccggagaa cttcaccaat tcatcacctg cattgagcag
    13801 attttgcagg ttctcaataa ccggtaatcc agccccaacg ttggtgtcat agaggaattt
    13861 acgccgcgat ttttccgccg cataacgcaa ctgatgatag taatccatcg acgaggtgtt
    13921 ggcctttttg ttcggcgtga caacgtggaa accttcgcgc aggaagtcgg catattgatc
    13981 cgccactgcc tggctggagg tgcagtcaac aatgaccggg ttcagcagat gatattcttt
    14041 cacgaggcga attaagcgcc cgagattaaa cggctctttg gcttgtgcaa gttcttcctg
    14101 ccagttttcc agattaaggc catgcacact agtgagcaga gccttcgagt tggcaacgcc
    14161 gcagacacgt aagtcgatat gtttattctt cagccagctt tgctgacgct tcagttgctc
    14221 cagcagcgca ccgccaacgc caccgacgcc aatcacaaac acttcgataa cctgatcggt
    14281 attgaacagc atctgatgag taacgcgcac gccagtggtc gcatcatcgt tatttaccac
    14341 gacagagatt gagcgttcag aagatccctg agcaatggcg acaatgttga tattggcgcg
    14401 ggccagcgcg gcaaagaatt tcgccgagat cccacgcaag gtgcgcatac catcacctac
    14461 caccgagata atggccagcc gttccatcac cgccagcggc tccagtaagc cttctttcag
    14521 ttccaggtag aactcttcct gcattgcccg ttcagcccgc acacagtcgc tttgcggaac
    14581 gcagaaactg atactgtatt cggaagatga ttgcgtaatc agcaccacgg aaatacgggc
    14641 gcgtgacatc gcggcaaaga cgcgcgccgc catgccgacc attcctttca tccccgggcc
    14701 ggaaacgctg aacattgcca tgttattcag attggaaatg cccttgaccg gtaattcgcc
    14761 ttcatcacga ctggcaccaa tgagcgtacc tggtgcttga ggatttccgg tatttttaat
    14821 caggcaaggg atctggaact gggcgatggg ggtaatggtg cgggggtgaa gaactttagc
    14881 gccgaagtag gaaagctcca tcgcttcctg gtaggacatc gacttcaaca acctcgcatc
    14941 gggcacctga cgcgggtcgc aggtatagac cccgtcaacg tccgtccaaa tctcgcaaca
    15001 atcggcgcgt aaacaggcag ccagcaccgc cgcggagtag tcggaaccgt tgcgtccaag
    15061 taccaccagt tcgccttttt cattaccggc ggtgaagcct gccatcagca ccatgtgatc
    15121 agccggaata cgacttgccg caatacggcg ggtggactca gcaatatcga cggtagattc
    15181 gaggtaatgc cccactgcca gtagtttttc gaccggatcg ataacggtaa cgttgtgacc
    15241 acgcgcttct aacacgccgg ccataatggc gatcgacatt ttctcgccac ggcaaatcag
    15301 cgcagcgtta atgctatccg ggcactgccc taacaaacta atgccatgca ggacatgttt
    15361 tatttgggca aattcctggt cgacgaaagt tttcaattgc gccagcggga atcccggctg
    15421 ggcggcggcg agtcccgtca aaagttcggc aaaaatacgt tcggcatcgc tgatattggg
    15481 taaagcatcc tggccgctaa tggttttttc aatcatcgcc accaggtggt tggtgatttt
    15541 ggcgggggca gagaggacgg tggccacctg cccctgcctg gcattgcttt ccagaatatc
    15601 ggcaacccgc agaaaacgtt ctgcatttgc cactgatgta ccgccgaact tcaacactcg
    15661 catggttgtt acctcgttac ctttggtcga aaaaaaaagc ccgcactgtc aggtgcgggc
    15721 ttttttctgt gtttcctgta cgcgtcagcc cgcaccgtta cctgtggtaa tggtgatggt
    15781 ggtggtaatg gtggtgctaa tgcgtttcat ggatgttgtg tactctgtaa tttttatctg
    15841 tctgtgcgct atgcctatat tggttaaagt atttagtgac ctaagtcaat aaaattttaa
    15901 tttactcatg gcaggtaacc agttcagaag ctgctatcag acactctttt tttaatccac
    15961 acagagacat attgcccgtt gcagtcagaa tgaaaagctg aaaaatactt actaaggcgt
    16021 tttttatttg gtgatatttt tttcaatatc atgcagcaaa cggtgcaaca ttgccgtgtc
    16081 tcgttgctct aaaagcccca ggcgttgctg taaccagtcg accagtttta tgtcatctgc
    16141 cactgccaga gtcgtcagca atgccatgac tcgttcgcgt aaagcttgca gttgatgttg
    16201 gtctgccgtt gtgtcacttt tcgccggttg ttgtattaat gttgctaatt gatagcaata
    16261 gaccatcacc gcctgcccca gattgagcga aggataatcc gccaccatcg gcacaccagt
    16321 aagaacgtca gccaacgcta actcttcgtt agtcagcccg gaatcttcgc gaccaaacac
    16381 cagcgcggca tggctcatcc atgaagattt ttcctctaac agcggcacca gttctactgg
    16441 cgtggcgtag taatgatatt tcgcccgact gcgcgcagtg gtggcgacag tgaaatcgac
    16501 atcgtgtaac gattcagcca gtgttgggaa aactttaata ttatcaataa catcaccaga
    16561 tccatgtgcg acccagcggg tggctggctc caggtgtgcc tgactatcga caatccgcag
    16621 ttcgctaaaa cccatcgttt tcattgcccg cgccgctgcc ccaatatttt ctgctctggc
    16681 gggtgcgacc aggataatcg ttatacgcat attgccactc ttcttgacca aataaccgcg
    16741 aacagggtga tcactgtcaa cttattacgc ggtgcgaatt tacaaattct taacgtaagt
    16801 cgcagaaaaa gccctttact tagcttaaaa aaggctaaac tatttcctga ctgtactaac
    16861 ggtttaattg ttaaaaaatg ctacatatcc ttctgtttac ttaggataat tttataaaaa
    16921 ataaatctcg acaattggat tcaccacgtt tattagttgt atgatgcaac tagttggatt
    16981 attaaaataa tgtgacgaaa gctagcattt agatacgatg atttcatcaa actgttaacg
    17041 tgctacaatt gaacttgata tatgtcaacg aagcgtagtt ttattgggtg tccggcccct
    17101 cttagcctgt tatgttgctg ttaaaatggt taggatgaca gccgtttttg acactgtcgg
    17161 gtcctgaggg aaagtaccca cgaccaagct aatgatgttg ttgacgttga tggaaagtgc
    17221 atcaagaacg caattacgta ctttagtcat gttacgccga tcatgttaat ttgcagcatg
    17281 catcaggcag gtcagggact tttgtacttc ctgtttcgat ttagttggca atttaggtag
    17341 caaacatgca gaccccgcac attcttatcg ttgaagacga gttggtaaca cgcaacacgt
    17401 tgaaaagtat tttcgaagcg gaaggctatg atgttttcga agcgacagat ggcgcggaaa
    17461 tgcatcagat cctctctgaa tatgacatca acctggtgat catggatatc aatctgccgg
    17521 gtaagaacgg tcttctgtta gcgcgtgaac tgcgcgagca ggcgaatgtt gcgttgatgt
    17581 tcctgactgg ccgtgacaac gaagtcgata aaattctcgg cctcgaaatc ggtgcagatg
    17641 actacatcac caaaccgttc aacccgcgtg aactgacgat tcgtgcacgc aacctgctgt
    17701 cccgtaccat gaatctgggt actgtcagcg aagaacgtcg tagcgttgaa agctacaagt
    17761 tcaatggttg ggaactggac atcaacagcc gttcgttgat cggccctgat ggcgagcagt
    17821 acaagctgcc gcgcagcgag ttccgcgcca tgcttcactt ctgtgaaaac ccaggcaaaa
    17881 ttcagtcccg tgctgaactg ctgaagaaaa tgaccggccg tgagctgaaa ccacacgacc
    17941 gtactgtaga cgtgacgatc cgccgtattc gtaaacattt cgaatctacg ccggatacgc
    18001 cggaaatcat cgccaccatc cacggtgaag gttatcgctt ctgtggtgat ctggaagatt
    18061 aatcggcttt accaccgtca aaaaaaacgg cgctttttag cgccgttttt atttttcaac
    18121 cttatttcca gatacgtaat tcatcgtccg ttgtaacttc tttactggct ttcattttcg
    18181 gcagtgaaaa cgcataccag tcgatattac gagtcacaaa catcatgccg gccagcgcca
    18241 ccaccagcac actggttccc aacaacagcg cgctatcgac agagttgagc agtccccaca
    18301 tcactccatc cagcaacaac agcgcgaggg taaacaacat gctattgcgc cagcctttca
    18361 ataccgcctg caaataaata ccgttcatta acgccccaat cagactggcg attatccatg
    18421 ccacggtaaa accgatatgt tcagaaagcg ccagcaagag caaataaaac atcaccaatg
    18481 aaagccccac cagcaaatat tgcattgggt gtaaacgttg cgcggtgagc gtttcaaaaa
    18541 caaagaacgc cataaaagtc agtgcaatca gcagaatggc gtacttagtc gcccggtccg
    18601 ttaattggta ttgatcggct ggcgtcgtca ctgcgacgct aaacgcgagg aagttttccc
    18661 agccggtatc attgcctgaa gcaaaacgct caccgagatt attagcaaac cagctgcttt
    18721 gccattgcgc ctgaaaacct gactcgctaa cttcccgttt ggctggcaaa aaatcaccta
    18781 aaaaactggg atgcggccag ttgctggtta aggtcatttc gctattacgc ccggcaggca
    18841 ccacagaaag atcgccggta ccgcttaaat tcagggccat attcagcttc aggttctgct
    18901 tccgccagtc cccttcgggt aaagggatat gcacgccctg cccgccttgc tctaacccgg
    18961 tgccgggttc aatggtcagc gccgttccgt taacttcagg cgctttcacc acaccaatac
    19021 cacgcgcatc cccgacgcta atcacaataa atggcttgcc aaaggtgata tttggcgcgt
    19081 cgagttcgct aagacgcgaa acatcgaaat cggcttttag cgttaaatca ctgtgccaga
    19141 cctgaccggt ataaatccct atcttgcgtt cttccacgtt ctgattgcca tcaaccatca
    19201 atgactcagg taaccaaaaa tggataaaac ttcgtttccg ctccacggtt ttatcctctt
    19261 cctgtaccgt ataaagctcg gtcacaggaa tagcgataag cggcccaaca agtttttgcg
    19321 gcccgctggt actttggcga atcgcatctt ccacatcgct acggtaatcg gcacgttcga
    19381 caatcacctg ccgaatcagc attatcggaa tcaacaacag caatactgca ccaaacaggg
    19441 tggtcatttt ccagaacagg gaggatttca acatagtctt ctcctttgca atggcagcag
    19501 gatacgaagg ctatgtggga agaatttgaa gctatgtgaa gtgacggtga agtcgaagcg
    19561 aggccagcac gccaccttcc tgcacgttgc gcagcgtgac ttcgccgtta aacaaacggg
    19621 cgacctcact gacgaacgcc aaccccagac cgctgctttt ttgcccattt gcacgcggca
    19681 aagagtaaaa gcgttcaaaa atacgtgaca gcgcgtagtc aggaatacca ctaccggtat
    19741 ccagcacctt aagcgtgacg tgttcctgat ccacttcggc gcttagcgtt atacgaccgc
    19801 tctcgggggt aaaatcgatg gcgttatcca gtaaattccc cagcgcctga tccagtaacg
    19861 ccggttcagc agcaacatta acctcggtgg gcgtaacatg tagagtgatg tttttttctg
    19921 ccaactgcac ggtgcgcgct tcgctgacgc ggcggaataa tgccgccaca tcaacagcag
    19981 tcaaaacgac ttcctgacga ttctccagtc ttgcctggcg tagtaacgtt tccaccagtg
    20041 cctgcattcg cgcattttgc gtcagaatgt tgtcagtaaa acgagccact acttcaggcg
    20101 gcggaccttc gcgtaaaatt tccgccgcgc cacgaatcgc cgccagtggg ctttttagct
    20161 catgggttaa cgcataaaca tactgctcaa tatagttttt cccttccagc ttcacgcgca
    20221 tactttccag cgcctgcgcg agtttacgca gctcgctact accgagatcg ggaagaggaa
    20281 cgggcttatt gtcagtgacg gaatcagcat agcgagtgag ccgggcaatg gagcggttga
    20341 tccaccaaac catgcctgcg ccaatcacca gtgcgatccc caacaaaatg gcgctggccc
    20401 ataaaattcg ccgctcgctg cgtttaatga ccggagccat cgccgcgttc ggtttgccta
    20461 cgctcaaaac gccaataagc cgcgagccgt ccataatcgg cgcggcaaca tacatcaccg
    20521 aactttcggg atcggcagga ttttgcaacg tgctacgcgc accatactga ccacgcaacg
    20581 ttagccagac gtcattccag cgcgaataat cctgcccaac ggctttattt gccgaatcga
    20641 acaatacttt gccctgcgca tcggtcatat agacatgata ttcattgcgc actttgttaa
    20701 tgccaccgat attggcgcga aacgggcgat gttgtagctg attaaacgcc tgcgccagtt
    20761 gcccatgcgt tgggtcccca gagagcaaat cgggacgcgc cagctccgcc agcaacgttg
    20821 cggtatcgat taacgtcccc tccgttgctc ttcgcacgcc cggtttaact tctttgacaa
    20881 aaatagccag tacgaaccag gccgccaccg ccaccagtaa aaaatagccc agcagcaacc
    20941 gcatgccgat acgcattaca ggcccctcag gctatatccc atgccgcgat gagtattaat
    21001 cggagaaaga tcggggttga tggcgcgcag cttggcacgc aacgttttaa tgtgggtatc
    21061 gacggtgcga tcgtaggtgt cctgcgcatc ttcccacacg ctatccatta gttgctggcg
    21121 ggaccagacg cggcctggcg acttgagtaa cgtcttcagc aataaaaact cataccgcgt
    21181 cagcgttaat ggcgtgtcaa accagctgat ctgcgccgcg ggttcattca attcaaaatg
    21241 accaatacgg atgacgggag acggcgacga gaacttcttc acccgacgca gtaaggtgcg
    21301 caccctggcg cacacttcgc ggggtgaaaa cggtttagcc acatagtcgt cagcgccaat
    21361 ttccagccca agcaggcgat cgacctcttc acttcgggcc gtcaggaaca gtacaggtaa
    21421 cgccggatgg agcgccagta actggcggca caattcaaag ccgctgatat caggcaaacc
    21481 aacatcgaga atcatgacgt cgggaacctg ctggcgagct ttatccagca ccggcaagcc
    21541 tcgctcaaag acctcgacgg caaaaccttc ctgctgcaac atgtagacca gcgtgtcggc
    21601 tatcccttgc tcatcttcca ctaaccagac cgtttcccgt tgcataaatc gcctctgtta
    21661 ttgccgccac ggcatgacag gaaccgcgct aatcgcgttt ttcggcgaac cttctacaac
    21721 tttgtcggag taagccagat aagcgagcgc gttgcgtttg gcgtcataaa agcgcacgac
    21781 ctgtaacgac ttaaagacca gggacgtgcg ttttttgaat actacctcgc cctgagcttt
    21841 gccgttttta atacgatccg acagttcaat cggcccgact tgctgacaag aaatagccgc
    21901 atcggaggta tcttccgcca gacccaatcc ccctttaata ccacctgttt tcgcccggct
    21961 cacataacag gtgacatttt tcacatcggg atcgtcaaag gcttccacaa caattttgtg
    22021 atccgggccg atcattttaa atacggtgtc gaccgaacca atctcttctg catgagccaa
    22081 tggccccagc attatcaggc ttaaagacag gatcaaatgc ttgtatttca tattgttacc
    22141 atttttatct attacacgag caattagttc gtcacggtaa ataacatatt gattaagaac
    22201 aaaatctcaa tacttttatt tccgtcaagc cctaaaacat actctactaa gaaaaaaaca
    22261 ctgaatgcta aaacagcaaa aaatgctatt atccaattac ctgatgtcag gtgctcgttg
    22321 ttgaaaggat gaggatattt tatggatcag gccggcatta ttcgcgacct tttaatctgg
    22381 ctggaaggtc atctggatca gcccctgtcg ctcgacaatg tagcggcgaa agcaggttat
    22441 tccaagtggc acttacagag aatgtttaaa gatgtcactg gccatgctat tggcgcgtat
    22501 attcgtgctc gtcgtttgtc gaaatcggcg gtcgcactac gcctgactgc gcgtccgatt
    22561 ctggacatcg cgctgcaata ccgcttcgac tctcaacaga catttacccg cgcattcaag
    22621 aagcagtttg cccagactcc tgcactttac cgccgttctc ctgaatggag cgcctttggt
    22681 attcgcccgc cgctacgtct gggtgaattc acaatgccag agcacaaatt tgtcaccctg
    22741 gaagatacgc cgctgattgg tgttacccag agctactcct gttcgctgga gcaaatctct
    22801 gatttccgcc atgaaatgcg ttatcagttc tggcacgatt ttctcggcaa cgcgccgacc
    22861 attccgccag tgctctacgg cctgaatgaa acgcgtccga gtcaggataa agacgacgaa
    22921 caagaggtat tctataccac cgcgttagcc caggatcagg cagatggcta tgtactgacg
    22981 gggcatccgg tgatgctgca gggcggcgaa tatgtgatgt ttacctatga aggtctggga
    23041 accggcgtgc aggagtttat cctgacggta tacggaacgt gcatgccaat gctcaacctg
    23101 acgcgccgta aaggtcagga tattgagcga tactacccgg cagaagacgc caaagcggga
    23161 gatcgcccaa ttaatctgcg ctgtgaactg ctgattccta tccgccgtta acgctgcagc
    23221 tcatctaatg caggggcgtc cagatgcgag atgtcccctg cggtttcaac gacccagcca
    23281 gacgccagcc acaggctttc ctgataatcc acgcgcgaaa tagaacagtt acgcagacgt
    23341 aagcggcgct ctgcccatgc tggtaatccg agaatcgtac tcaccagaca tcccagtgca
    23401 ataccatggc tcaccagcag cggtcggctt ccctgcggta agtcccggca agattccagt
    23461 gccgcattga cgcgatcgct gagctcctgc attgactcgc cttcaggaat acgcccgtca
    23521 acggtgccat tgaccagctg ccgacgccag ttctcttctt cttcggtcag agaatcgata
    23581 tgtctttttt ccagcacacc catgtttaat tcacgcaggc gagaatcaaa gatgatgtca
    23641 cagccgcagg cctgggcgat gatttccgcc gtacgccggg tgcgtcctaa atcgctactg
    23701 ataatatgcg taatgccaag ctctttggca cgggttgcca cctgcatcgc ctgttgctca
    23761 cctttggcgg tcagcgggct gtcagactgg ccctgaatac gtcgctcggc gttccactgc
    23821 gtttcaccgt ggcggactag gtatacctgt aacatgcttt ttttccgtat actgctgtaa
    23881 cgttaatttt ctgagtaatg ctgattatgc accaagttat ctgtgcgacc accaatcccg
    23941 ctaaaattca ggccattctg caggcatttc acgagatctt cggcgaagga tcctgccata
    24001 ttgcatccgt tgccgtcgag agcggtgtac cggaacagcc ctttggcagt gaggaaacgc
    24061 gcgctggcgc acgaaatcgg gtagcaaatg cccgccgttt acttccagag gctgattttt
    24121 gggtggcgat tgaagctggc atcgatggtg acagcacttt cagttgggtg gtgattgaaa
    24181 acaccagcca gcgcggcgag gcgcgttcgg ctactttgcc gttacctgcg gtgattctgc
    24241 agaaagtgcg tgaaggtgag gcgcttggtc ccgtgatgtc gcgttatacc ggtattgatg
    24301 agattggtcg taaggaaggg gcgatcggcg tgtttaccgc cgggaaactc actcgcgcca
    24361 gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt tcacaatgcc gtgtatcagg
    24421 cattgtagga cggataaggc gttcacgccg catccggcat tcggtgcacg atgcctgatg
    24481 cgacgctgcc gcgtcttatc aggcctacaa aatcaatcgc ttttcagcaa cacctcttcc
    24541 agccactggc gcagctccac gggcgcggct ttcaggctgt tagatccacg cgtaatcgtc
    24601 gcgatgcccg cgccgagttc attttttaac tcacgctgac tcatttcgcc acgcaacagc
    24661 tcttcgacaa tacgcacgcg agtccccaac gcttcgcgct catctggcgt cagcatcagg
    24721 tttaacaacg gtaaatggag atcgttttgg taggcattct taagcaggtc gacaaaacgt
    24781 aaccactcct ggtgacgctg ttctgccatc gctgctgaat agggtgattg ttgggccata
    24841 atatgtcgcc attgttagcg ggggaagcaa aatgcctccc ccggttgtac tcgctaaaga
    24901 gtacgatagc atatcataaa cgtgcggatc agtaacgacg tccccattcc gtggcgctca
    24961 tcaacgtcgg tttatccccc atgaaataac ggtagtaagc gtcataagcc agcacgttct
    25021 tcacataacc gcgcgtctcg gagaacggaa tactctcgac aaatgccaca gcgtcgatac
    25081 gcccggcgct gttgccaagc caggttcgca cccgtccagg tccggcgtta taagctgctg
    25141 aggagaaaat acggttattg ccaaactgct gataaacata ttgcaggtaa ctggtgccaa
    25201 tgttgatatt cgtttccgga tccagcaatt gcccagggct gctataacca ggaatagaga
    25261 acatcttcac cgtatgggtc gctgtaccag gcataatctg catcaggccg ctggccccta
    25321 ccggtgattt cactttcgga ttccaggcgc tctcctgacg ggcaatcgcc atcgcatagc
    25381 tttgcgggat ctctttccca ctggtgtaac gtttgaaaag atcgttgtaa gccagcggga
    25441 atcgctcttc cagatgatcc caaagcttcc cggcgatcgt tgcctgaacg ctaagatccc
    25501 accattggtt attgaaagca taccgagcca gttgagcctg ctctgttttt gacttgctct
    25561 tcaccagatt ggcccactcg ctacgcgcgg tgttatcgag attccagtac atcaactcgc
    25621 gcacgcgcgc catctccggc ccctgagtca gggcgctgtc aacattctgc ggcgctttat
    25681 caatcttcag ctcatactct tctccgatgc gttgtgctgc aaccatcggg tagaaaccac
    25741 gctgttgcat gagttgatgc aaaatctctt tcgcttcagc ttcacgtccg cgttccagca
    25801 ataaatccac ctgccagtaa cgccattcat ctttctcttt tgcttccatc ggcagacgcg
    25861 ccagccaggt attcaggccg cgacgatcgc cggtgccaag tgccattcgc acacggcgtt
    25921 caatcaacga cgtggattgc gagcgcataa tgacgtcatc gcgccatttc gcctgctcgt
    25981 cggtgacatc gttgcccatc aaacgccagg cgacgatatc gcgcagctcc tgaatctgat
    26041 cttcattaag ctgctgcacc tgggcaagcg acgggatcat cagccgtgca ttttcagcat
    26101 cctgccgcgc cacactggca aacgccaccg ctgccatttg acgggtaaaa tcggtcgctc
    26161 cggttgtacg cgcgaaggtc agtaccgtat tagggttgtt cgccagtgaa atgattgccg
    26221 aggcgatagt ctggtaatcg gcaggcatct gccctgccag cactgttacc aggccagtgt
    26281 tacccgcttt catcgccaga cggatacgct ctaaatacgc cagcggatct tgtttacctg
    26341 acgcacgcca gacgctaaat aacttgtcac aggcgttagg ctggctcttg ccggttagcc
    26401 acagctcttt cgccccttgc caggcttctt cactctgccc ggtgttccat ttcgcatagt
    26461 agtaattaca ttgcgcttcg gtagttccgg gcttttccgg gctaaaggct aacaagccac
    26521 gccagtcttc acgccgcgcc agttcattga cgaaacgaga ttgcagcgtg cgagcgggag
    26581 gaagcgtggg gttagcgcga acaaagttag tgaccgtcac cgccggttga ttcatcagat
    26641 catcggtgat ctggcggtat tccaggtagg gataaagcgg ataatccttc agtccaggca
    26701 tcatttgttc gaccacatcc atttgtcgat tatcccaggc ctgcttgatt tgcgcgtaac
    26761 ggctacgctg ctcatccagt gagtcggctc gcgccacgct gctgaccgtc agcagacaga
    26821 caccggcagc caacagccgc caggtaactt gtttggcttt ttccacaagc gcatcctcta
    26881 agtgtaaata catcaatgca gcgtcatgcc gcgtaataga tttatgctaa ccagtcattg
    26941 ccgtttacgc cacgttacgg acactttttt acttttactg cgaaggtgat cgaccaactg
    27001 ggattaggcg gggaaaaagg ctacacttcg cctttgaaaa ctcatcatca cgataaacat
    27061 agaggcgaag tccaacgtgg ctcaattcgt ttataccatg catcgtgtcg gcaaagttgt
    27121 tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt ttcttccctg gggcaaaaat
    27181 tggtgtcctg ggtctgaacg gcgcgggtaa gtctaccctg ctgcgcatta tggcgggcat
    27241 tgataaagac atcgaaggtg aagcgcgtcc gcagccagac atcaagattg gttacctgcc
    27301 gcaggaaccg cagctgaacc cggaacacac cgtgcgtgag tccattgaag aagcggtttc
    27361 tgaagtggtt aacgccctga aacgcctgga tgaagtgtat gcgctgtacg ccgatccgga
    27421 tgccgatttt gacaagctgg ccgctgaaca aggccgtctg gaagagatca ttcaggctca
    27481 cgacggtcat aacctgaacg tacagctgga gcgtgcggcg gatgcgctac gtctgccgga
    27541 ctgggacgcg aaaatcgcta acctctccgg tggtgagcgt cgtcgcgtag cgttgtgccg
    27601 cctgctgctg gaaaaaccag acatgctgct gctcgacgaa ccgaccaacc acctggatgc
    27661 cgaatccgtg gcctggctgg aacgcttcct gcacgacttc gagggcaccg tggtggcgat
    27721 tacccacgac cgttacttcc tcgataacgt tgcaggctgg atcctcgaac ttgaccgcgg
    27781 tgaaggtatt ccgtgggaag gcaactactc ctcctggctg gagcagaaag atcagcgcct
    27841 ggcgcaggaa gcttcacaag aagcggcgcg tcgtaagtcg atcgagaaag agctggagtg
    27901 ggtacgtcag ggaactaaag gccgccagtc gaaaggtaaa gcacgtctgg cacgctttga
    27961 agagctgaac agcaccgaat atcagaaacg taacgaaacc aacgaactgt ttattccacc
    28021 tggaccgcgt ctgggcgata aagtgctgga agtcagcaac ctgcgtaaat cctacggtga
    28081 tcgcctgctg attgatgacc tgagcttctc gatcccgaaa ggggcaatcg tcgggatcat
    28141 cggtccgaac ggcgcgggta aatcgaccct gttccgtatg atctctggtc aggaacagcc
    28201 ggacagcggc accatcactt taggtgaaac ggtgaaactg gcgtcggttg atcagttccg
    28261 tgactcaatg gataacagca aaaccgtttg ggaagaagtt tccggcgggc tggatattat
    28321 gaagatcggc aacaccgaga tgccaagccg cgcctacgtt ggccgcttta actttaaagg
    28381 ggttgatcag ggtaaacgcg ttggtgaact ttccggtggt gagcgcggtc gtctgcatct
    28441 ggcgaagctg ctgcaggttg gcggcaacat gctgctgctc gacgaaccaa ccaacgacct
    28501 ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg gagttcccgg gctgtgcgat
    28561 ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc acgcacatcc tggactacca
    28621 ggatgaaggt aaagttgagt tcttcgaagg taactttact gagtacgaag agtacaagaa
    28681 acgcacgctg ggcgcagacg cactggagcc gaagcgtatc aagtacaagc gtattgcgaa
    28741 gtaatgcgta aaatgccgga tgcggcgtga acgccttatc cggcctacaa accaagaaat
    28801 ttcaatacgt tgtgaatctc caggcctgat aagacgcggt aagcgtcgca tcaggcattg
    28861 agcaccgaat gccggatacg gcgtgaacgc cttttccggc ctacaacagc cttagtatcc
    28921 gcaatttcgt agcgggtcac ccgtgcaggt tttttcaaac cttgtttaat ctcgacagcc
    28981 tcttccagag aggttttcaa ctcatcaaaa aacttatttt atacctcgcc tttgagccgt
    29041 ttcatcgcgg ttatctctgc tcccccatca tctcccgcac cagctccacg cagcgcagga
    29101 aacggctgtc ataatcgtcc tcttcaaccc gcacgaattc gatattgttc tcttccagca
    29161 tctccaccag caagttctgg aactctttgc gatccaccga actgccgagg ctgcgtaaac
    29221 catccgccac ccacggcgtg ttgttctcca gcaggatcac cagatcgaaa cggtattcat
    29281 caatcaacgc ctgtacgaac ggatgctcac gcccttcgta ctttttgcag aacgcctggg
    29341 tagtgacaaa atcagtgtca ataaacgcca ctttattggc atatttcact gcaaaatcaa
    29401 tgtattgtgc gtggcccagc gcgattttat cgtaatcgga atactgcaat gcgatctcat
    29461 caccgccgag gtgtgaaaag acataatcgc gaccatattc ccacgcactg gtggtattga
    29521 agatattggc aagtttgttt accagggtgg atttaccgct cgactcgcca ccaaggatcg
    29581 ccacggtacg tacaaagaac ggcttcactt ccgtaggaat atattcccag tagcggaacg
    29641 ggttttcgcg gatctgcgca ccgctgatac tcataaaggt acgtttcgga tcgaccagca
    29701 ccgtatcgat ccccagatgt tccatatact gtggcgcatc ggcttcttcc gaggtgtaga
    29761 tcaggtccgg ctgaatcccc ttttcagcca taaacttttt gatgccgttg ctccacacat
    29821 cccagccgtg cggatacggc tccatgccct cttcgttgaa agcatgaatg cgaatatttt
    29881 tctgatattt aaaagtttgt aataaccagc gcagacgatc cggcaccgtg ggctgctgcg
    29941 acatggcgct gtcttcaaac aacgcgcggt cgcgggtatc gtcaaaaccc ataatgatat
    30001 gcagctcgtc aacctggcta caggcgcgct ggataaggta gatatgtccg gtatgcagcg
    30061 ggtagaactt accgaatacg acaccaatgg ttttcttctg tcggggaaat tcaagcccca
    30121 aaaaacggtg caacgcctcc agcttttgcg cgctggggct tttaattttg gcattcagta
    30181 actggcttaa ataccctttg gtcataccgc tggcatcagc tacctgctgt agcgtgcagc
    30241 cctgttgctt gatggcagtt ttcaggtaat caaatgacga catatctccc tccgtatctc
    30301 tcattataag tcgtcgaaca cgctaagcgc gtcggagagt tttttaacgc caaaaatctg
    30361 catcccttcc ggcgcttttt tcggtacgtt agccgccgga acaatcgccc ggcgaaaacc
    30421 gtgtttcgcc gcttctgaaa ttcgttcctg accgctgggc accgggcgga tctcccctgc
    30481 cagcccgact tcaccaaaca ccaccagatc ctgtggcagc ggtctgtcac gcaggctgga
    30541 aaccatcgcc agcagtaacg ctaaatcggc actggtttcg gttaccttca cgccgccgac
    30601 cacgttcaca aatacatcct gatcggccat ttgcagacca ccgtgacggt gcaagacagc
    30661 cagcaggatt gccagacggt tttgttccag ccccactgcc acgcggcgcg gattcgccat
    30721 catcgagtga tcgaccagcg cctgaatctc caccagcaac ggacgcgttc cttcccacac
    30781 caccatcacg gagctgccgg aggtcacttc atctccgcga cttaagaaaa ttgccgaagg
    30841 gttgctgact tcacgcagcc cctgttcggt catcgcgaag acgcccagct cattcaccgc
    30901 gccgaagcgg tttttatggc tgcgcaaggt gcgaaaacgg gagtcggcat caccatccag
    30961 aagcaccgaa cagtcgatac agtgttccag cactttaggg ccagccagcg agccatcttt
    31021 ggttacgtgc ccaaccatga caatcgccac accgcgcgtt ttggcgaagc gcgtcagata
    31081 agccgccgtt tcacgcacct gcgccacgct accaggcgat gattgtacat ccgccatatg
    31141 catcacctgg atcgagtcaa ttaccatcag tttcggttgc tcttcttcgg caatcaggca
    31201 gatctgttcg atgctggttt ccgacaacat attgagattg tcagtcggca ggccaaggcg
    31261 atgagcgcgc attgccacct gttgcagcga ctcttcgcca gtgacataca gcgttttcat
    31321 ctgctgggcc agtttgcaca gcgtttgcag tagcagcgtg gatttccccg cacctgggtt
    31381 accgccaatc agaatggcgc ttcccggcac cacgccgccg cctagtacgc ggtcgaactc
    31441 tttaaatccg gtggaaaaac gcggcagctc ttcaaggctg atatcggaga gtttctggac
    31501 ttttgccacc ccggcgctac cggcatagcc gctgagacgc tcgttacgcg ccaccgttgg
    31561 cgacgcagca agacgcacct cggtgatggt gttccaggca tgacaggcac tgcactgccc
    31621 ctgccagcgc ggataatcgg ccccgcattc attacaaaca aaggcgcgtt ttggagcttt
    31681 tgccacggtg tacctcgtta atgctgtgcc gcccgcagga tggcgggcga gcaattactt
    31741 ctgattcagg ctgccagaga ggatgcagaa tacccccatc aggtcagcgt gacggatggt
    31801 gacttccgcc ttttcattca cttttggctt ggcatggtag gcaatcccca gccctgctgc
    31861 tttgatcatc ggcaggtcat tagctccatc gccaatcgcc acggtctgcg ccagcgggat
    31921 ttcatactcc tgcgcgaggc gagtcaaagt tttcgctttg tactgcgcgt ctacgatgtc
    31981 gccgatcaca ttgccggtaa atttaccgtc catgatctcc agttcattgg ctaccacggc
    32041 ggtcaggcgc agcttgtcgc gcagatattc agcaaagaaa gtaaagccgc cggaggcaat
    32101 cgccactttc cagcccagcg tttccagctt gagcaccagt tgcgttaagc ctggcatcag
    32161 cggcagattt tcacgcacct gttgcagaat attggcgtca gcgcctttca gcgtcgccac
    32221 acggctgcgc aggctggcgg taaaatcgag ttcgccgcgc atcgcccgtt cggttacttc
    32281 cgccaccatc tcgcccgttc cggccagttt ggcaatttca tcaatacatt caatctggat
    32341 ggcggtggaa tccatgtcca tcaccagcaa acccggcgtg cgcaggtgcg ggattttccc
    32401 cagcggggcg acatccagtt gcgcttcatg ggccaggcgc gtagcccgtg cggtgagtga
    32461 acctgccaga cgaatcacct gataatcttc cacgcaccag gcggcaacaa tcaccatcgc
    32521 cgcacccagt ttgctctggt attgggtcag acgctgttta tccagcccac gaccatacag
    32581 cagccagccg ctacgacctg cgtggtaatc cagtggcatc acttcatcac cacttaatga
    32641 aagaggcaga cccggccata aagagacatc ttcaggcagg tcgcaccagg taatgttagg
    32701 cattaaggct cctgtaaaat cgttcgaagc agggaaaata acgcatgagg ctaccttgta
    32761 tccattgctt ctggcaacat taagtctcaa attttcaaag ggtggaagat ggctcgcaca
    32821 aaactgaaat tccggctgca tcgggcagtg attgtcctgt tctgtcttgc cttgttagtg
    32881 gcactgatgc agggggcgtc atggtttagt caaaaccacc agcgacagcg taatccacag
    32941 ctggaagaac tggcccgcac cctggcgcgt caggtgacgc tgaacgttgc accgctgatg
    33001 cgtaccgact caccagatga aaaacgcatt caggcgatcc tcgatcagtt aacggatgaa
    33061 agccgcatcc tcgacgcggg tgtgtatgac gaacaaggcg atcttatcgc ccgttcaggc
    33121 gaaagcgtcg aagtgcgcga ccggctggcg ctcgacggta aaaaagcagg cggctatttt
    33181 aaccagcaga ttgtcgagcc aattgcgggt aaaaacggac cgctcggcta tctgcgcctg
    33241 acactcgaca cccatacgct cgccaccgaa gcccaacagg tggataacac cactaacatt
    33301 ttacgcctga tgttgctgct ctcactggca atcggcgtag tgctgacccg cacgctgcta
    33361 cagggtaaac gcacccgctg gcagcaatcg cccttcctgt taaccgccag caaaccggtg
    33421 ccggaagagg aagaaagcga gaaaaaagag tgacccatta ctacaagaaa ggaaatcgtt
    33481 atgtccacat tacgcctgct catctctgac tcttacgacc cgtggtttaa cctggcggtg
    33541 gaagagtgta tttttcgcca aatgcccgcc acgcagcgcg ttctgtttct ctggcgcaat
    33601 gccgacacgg tagtgattgg tcgcgcgcag aacccgtgga aagagtgtaa tacccggcgg
    33661 atggaagaag ataacgtccg cctggcgcgg cgcagtagcg gtggcggcgc ggtgttccac
    33721 gatctcggca atacctgctt tacctttatg gctggcaagc cggagtacga taaaaccatc
    33781 tctacgtcga ttgtgctcaa tgcgctgaac gcgctcggcg tcagcgccga agcgtccgga
    33841 cgtaacgatc tggtggtgaa aacagtcgaa ggcgaccgca aagtctcagg ctcggcctat
    33901 cgcgaaacca aagatcgtgg cttccaccac ggcactttgc tgctcaatgc cgaccttagc
    33961 cgcctggcaa actatctcaa tccggataaa aagaaactgg cggcgaaagg cattacctca
    34021 gtgcgttccc gcgtgaccaa cctcaccgag ctgctgccgg ggatcaccca tgagcaggtt
    34081 tgcgaggcca taaccaaggc ctttttcgcc cattatggcg agcgtgtaga agcggaaatc
    34141 atctccccgg acaaaacgcc agacttgcca aacttcgccg aaacctttgc ccgtcagagt
    34201 agctgggaat ggaacttcgg tcaggctccg gcattctcgc atctgctgga tgaacgcttt
    34261 agctggggcg gcgtggaact gcatttcgac gttgaaaaag gccatatcac ccgcgcccag
    34321 gtgtttaccg acagcctcaa ccccgcgccg ctggaagccc tcgccgggcg actgcaaggc
    34381 tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag cgctgttggt tgacttcccg
    34441 gaccaggaaa aagagctacg ggagttgtcg acgtggatag cgggggcggt aaggtaactc
    34501 gctgattaaa ataccaaagg aagggaatat gatcgatctg gaaaaccagg agcgggagat
    34561 tatcaattta atgttaagcc agcgtatttc ctggctagcc gccgtgcgta tcaggcataa
    34621 attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg attaattctc taaaatagat
    34681 cgaaaagacg gaacggctta gctcaaacat caaaagcaaa atgggagaaa tttatggttg
    34741 tccaccagag ttgctgatat gcccttcctg gatgacggct gaacataaat gaatagattc
    34801 gtgaatagat acttgaatgt attttatttt ttatccatat gaaaaataat agtaaatcta
    34861 taaatcagtt gctattgatg aatagatact aaaaagccgg agcagtcccc cactccggct
    34921 taacacgaca atcgcctttc agcgaaacac aattactctt tatcgcccag cagaacggat
    34981 tccagtgcga ttttgatcat gtcgttgaag gtggtctgac gctcagcggc agtggtctgc
    35041 tcgtgagtgc ggatgtggtc agacacagtg cagatggtca gggctttcgc gccaaattct
    35101 gcagcgacgc cgtagatacc agccgcttcc atttccacgc cgaggatgcc gtatttttcc
    35161 atcacgtcga acatttcgcc gtccggagag tagaacaggt cagcggagaa caggttacca
    35221 acgcgagcat caatgcccag tgctttagcc gcgtctaccg cgttacgcac catgtcaaag
    35281 tcagcgatag cggcaaagtc atgatcttta aaacggatgc ggttaacttt ggaatcggtg
    35341 caggcaccca taccgataac gacgtcgcgc agttttacgt gcggcagaac tgcgccacag
    35401 gaacccacgc ggataatttt cttcacgccg aaatcggtga tcagttcttt ggtgtagatg
    35461 gagcaggacg ggatacccat accgtgaccc attacggaaa ttttgcggcc tttgtaagta
    35521 ccggtgaagc ccagcatacc gcgaacgttg ttcacttcac gggcatcttc aaggaaagtt
    35581 tcagcaatat acttcgcacg cagcgggtcg cctggcatca aaactacgtc agcgaaatcg
    35641 cccatttctg cattaatgtg tggggtagcc attgttttat ccttatcaac atgttttcac
    35701 cctctccctg agggagaggg ccggggtgag ggcatcaagc cgcacccatt atcagaacat
    35761 ggctttgcca tattccatat cagaagtacc aaaatatttt gccagagtct ggccgatatc
    35821 cgcgaaggtt tcacggtgac ccagtgagcc cggttttact ttcgggccgt aaaccagtac
    35881 cggaatgtgt tcacgcgtgt ggtcagtacc ggtccaggtc ggatcacaac cgtggtcagc
    35941 agtgaggatc aggatgtcgt catcgcgcag cagagacatc agctccggca gacggcggtc
    36001 gaacagctcc agacccacag cataaccggc gacgtcgcga cggtggcccc aggaagagtc
    36061 gaagtcaacg aagttggtga agacgatggt gttatcaccc gcttctttca tctctttgat
    36121 ggtggcgtca aacagcgcgt ccaggccagt tgctttcact ttcttggtga taccgcagtt
    36181 ggcgtagatg tctgcaattt taccgacaga aaccacctga ccgtgttttt catcaaccag
    36241 tttctgcagc acggtcggtg ctggcggctc aacagccagg tcgtgacggt taccggtacg
    36301 ctggaagtta ccggctttgt caccgataaa cggacgagcg ataacacgac cgatgttgta
    36361 gccgccgttg gtcagctctt cacgggcgat ttcgcacagt tcgtagagtt tatccaggcc
    36421 aaaggtttct tcgtggcagg caatctggaa cacagagtca gcggaggtat agaaaatcgg
    36481 cttgccggtt ttcatgtgct cttcgcccag ctgatccaga atgaccgtac cggaagagtg
    36541 gcagttaccg aggtaacccg gcagattagc gcgttcaacc agtttatcca gcagctcttg
    36601 cgggaagctg ttttcgtgat cggagaaata tccccactca aatagaaccg ggacaccggc
    36661 gatttcccag tgaccagacg gggtatcttt accggatgac atttcgtgcg cccatgcgta
    36721 cgcgccgata acttcagcgt tgccgtccat tcccgccgga atgaaaccgg tagaaccttc
    36781 gtgtgctttc gccagcccca gacgggtcag atttggcaga ttgagcggac ctttacgacc
    36841 gttatcagct tcgcctttgg cacaagcttc tgcgatatga cccagggtgt cagccccgac
    36901 gtcaccaaag cgttctgcat cttctgtagc gccgatgccg aatgagtcca gcaccataat
    36961 aaatgcacgt ttcatatgtt ctccgtactc agtgcttcaa atttaaatga tcagatcagt
    37021 ataccgttat tcgctgatac ggcgatagac agttggtgtg ctttccggtg ctttatcggc
    37081 aagtttaatt gccgctttca ccgctttcgc cgcttcctgc cagctgtttt cgtctttcgc
    37141 gtggataacc gccagcggac gctgaccgtc tacctggtcg cccaaacgcg ccatatcagt
    37201 aaagccgacg ctgtaatcaa tggtgtcaga tgcctgacga cgaccgccgc ccattgcaac
    37261 cactgccatc cccagcgcgc gggtatccat ttcactgaca aaaccttcgg tatcagcata
    37321 gactgctttc gtcagcatcg ctgtcggcag atacttcgcg tagttctcaa cgaagtcggt
    37381 tgggcctttt tgtgccgcta ccatacgacc aaagacttct gctgctttac cgttgtccag
    37441 cactgcttgc aatttcgcgc gcgcttcggc gtcatctttc gccagtttgc cggagataag
    37501 catctccacg cacagcgcca tcgtgacatc aaacagacgc gggttacgat actcacccgt
    37561 caggaactgc accgcttcac gaacttcaac cgcgttacct gcactggagg ccagtacctg
    37621 attcatgtcg gtgagcagcg cggtggtgcg cacgccagcg ccgttagcca cgccaacaat
    37681 cgcttcggca agggcttcag agagttcgta ggtcggcata aacgcgccgc tacccacttt
    37741 cacgtccatc accagcgcgt ccagaccttc cgcaagtttc ttcgccagaa tagaggcggt
    37801 gatcagcggg atggagtcca cggttgcggt aatatcacgg gtcgcgtaga aacgtttatc
    37861 cgccggagcc agtgagctgg tctgaccgat aatcgccacg ccgacgtctt taataatttc
    37921 gcggaaacgg ttgtcatccg ggaaaatgtc gaagccaggg atggattcca gtttgtcgag
    37981 cgtaccgcca gtatgaccga ggccgcgacc agagatcatc ggaatatagc cgccgcaggc
    38041 tgcgaccatc ggccccaaca tcagcgaagt cacatcgccg acgccaccag tggaatgttt
    38101 atcaacaatc gggccattca gatgcaggct tttccagtcg agaacggttc ctgaatctcg
    38161 catcgccatg gtcagcgaga cacgctcagg cattgtcata tcgtggaaga aaatggtcat
    38221 cgcgagggcg gcaatctgcc cttcggagat agtgttgtcg cgaatgccgt tgataaagaa
    38281 acgaatttct tcatcgctca gtgcatgacc atcacgtttt ttacgaataa tttcttgtgc
    38341 gagaaacaag gtaacctccc gaggaaaaga gtatgaggcg gcttttgccg gatgcgtcta
    38401 cgccttatcc ggcctacggg tagatacgtc tcgtaggcct gataagcgca gcgcatcagg
    38461 cgtaaagcat cttacttagt agctgctggc gctcttaccg tcaccgtggc ccagcgcttt
    38521 caacaggctt gccagcaggc tggaagcacc aaagcggtag tgacgcgcat ctgcccagtc
    38581 agcaccgaac agttcatctg cgatggcgag atatttctgc gcatcttccg cagtacgcac
    38641 gccgcccgct ggtttgaaac caacggtttt ttctacgccc atatcacgga tcacttccat
    38701 catgatgcgc gcgctttccg gcgtcgcgtt cacagccact ttaccggtag aggttttgat
    38761 gaagtccgca cccgctttga tggagatttc agacgcttta cggatcagcg cttcgtcttt
    38821 cagttcgccg gtttcgatga tcactttcag cagtacattc gctgccgcgc aagcctcttt
    38881 acaggctttc accaggtcaa aaccaacctg ctcgttaccc gccatcagcg cgcggtacgg
    38941 gaacacaacg tcaacttcat cagcaccgta ggcgattgcc gcacgggttt ctgccagcgc
    39001 gatttcgatg tcatcgttac cgtgtgggaa gttggttacc gtagcgattc ggatttccgg
    39061 ggtgccctgc tctttcagcg ttttgcgagc aatcgggata aagcgaggat agatacagat
    39121 agcggcggta ttgccgaccg gggttttggc ctgatgacac agagcaatta ctttctcgtc
    39181 ggtgtcgtcg tcattcaggg tggtcaggtc catcaatttc agtgcacgca ggctgcttgc
    39241 tttcagatca gtcatttcat tctcctggct tgtcgccaat aaaattcacc ttgcgagttt
    39301 gttagtattc taacatctac tccgcaacac acttcgatat acatcacaat taaggaaatc
    39361 tacttacaag tttgcatcac tgtaatgcga tctggttcaa ataattcact ttcaaatgaa
    39421 tgcgtcagtg gtggcaaacg catcaggatc aaagtgaaca tcacgaaact tcttacaatg
    39481 gcgcatcacc ttcggcataa aaggaaaaga agatgcccac ttctcatgaa aatgcactgc
    39541 aacaacgttg ccagcaaatt gtcaccagcc cagtgcttag cccggagcag aagcgccatt
    39601 ttctggcact ggaagcagaa aacaatctgc cttatccgca gcttcctgcc gaagcccgcc
    39661 gcgcgctgga tgagggtgta atctgcgata tgtttgaagg tcatgcgccg tacaaaccgc
    39721 gctatgtctt acccgattac gcccgttttc tggcgaacgg ttccgaatgg ctggagctgg
    39781 aaggcgcgaa agatcttgat gacgcactct ctctgctgac cattctttac caccacgtac
    39841 cgtcggtcac atcgatgccg gtctacctgg ggcaactgga tgcgttgttg caaccatatg
    39901 ttagaattct aacacaagac gagatcgatg ttcgaataaa acgtttctgg cgttacctcg
    39961 acagaaccct gccagacgcc tttatgcacg ccaatatcgg cccgtctgat tcgcccatta
    40021 cccgcgcgat cttacgtgca gacgcagagt tgaagcaggt ttcaccgaac ctgaccttta
    40081 tctacgatcc tgaaatcacc cctgatgacc tgctgctgga agtggcgaag aacatctgtg
    40141 aatgtagcaa accgcacatc gccaacggtc cggtgcatga taaaattttc acaaaagggg
    40201 gctacgggat tgtgagctgt tacaactcac tgccgctggc gggtggtggc agcacgctgg
    40261 tacgccttaa cctgaaagcc attgccgagc gcagcgaatc gctggatgac ttctttacgc
    40321 gcactctacc gcactactgc cagcagcaga tcgccatcat cgatgcgcgg tgtgaattcc
    40381 tctatcaaca atcacacttc tttgagaata gcttcctggt gaaagaaggg ctgattaacc
    40441 ctgaacgttt tgtgccaatg tttggcatgt atgggctggc ggaagcggtt aacttgctgt
    40501 gtaagaaaga agggattgcc gcgcgctacg gtaaagaagc cgccgcaaat gaagtgggtt
    40561 atcgcatcag cgcgcaactg gcggagtttg tcgccaatac ccccgtgaaa tatggctggc
    40621 aaaaacgcgc catgttacac gcacagtcgg ggatcagttc cgatatcggc accacgccgg
    40681 gcgcgcgttt gccgtatggt gatgagccag atccgatcac ccatctgcaa actgtcgccc
    40741 cgcatcatgc ttattattat tccggcatca gcgacattct gacgctcgac gaaaccatca
    40801 aacgtaatcc gcaggcgctg gtacagcttt gcctcggtgc ctttaaagcc ggaatgcgtg
    40861 aatttactgc caatgtcagc ggtaacgatc tggttcgcgt taccggttat atggtgcgtt
    40921 tgtcggattt agaaaaatat cgcgccgaag gttcacgcac caacaccacc tggctgggcg
    40981 aagaagccgc acgcaacact cgtattctgg aacgccagcc gcgcgtgata agccatgaac
    41041 agcagatgcg ctttagtcag taagattatc cccttctcct gcgttgacgg gccaggcagt
    41101 cgtctggctc tgtttttgca gggctgcaat ctgcgctgca aaaactgtca caatccgtgg
    41161 acgatgggac gttgcaatga ctgtggggag tgcgtgccac agtgtccgca tcaggcgttg
    41221 cagattgttg acggcaaagt ggtgtggaac gctgtggttt gcgagcagtg tgatacctgc
    41281 ctgaagaggt gtccgcaaca tgccacgcca atggcgcaat ccatgagcgt ggacgaagtg
    41341 cttagccatg tccgcaaagc ggtgctgttt attgaaggga taacggtaag cggcggcgaa
    41401 gccacgaccc aactgccgtt tgtagtggcg ctgtttactg ctatcaaaaa cgatccgcaa
    41461 ctgcgccacc tcacctgcct ggtggacagt aacggcatgt tgagcgaaac cggctgggaa
    41521 aaattactcc cggtgtgtga cggcgcaatg ctcgatctca aagcgtgggg gagcgaatgt
    41581 catcaacact taaccggacg cgataatcag cagattaagc gcagcatctg tttgcttgca
    41641 gagcgcggca agctggcgga actgcgtttg ctggtaattc cagaccaggt ggattatttg
    41701 catcacatcg atgagctggc gacgtttatc aagagacttg gcgatgttcc ggttcgcctg
    41761 aatgcgtttc atgcccacgg cgtgtatggc gaggcgcaaa gctgggcgag cgccacgccg
    41821 gaagacgttg agccgttggc tgatgcgtta aaggtgcgcg gggtgagccg gttgatattt
    41881 ccggcgctct atttgtgaca tgccggatac ggcctacggt tcggcacaga cttgtaggca
    41941 tgataagacg cgacaagcgt cgcatcaggc attatgcgcc gactgccgga tgcggcgtga
    42001 acgccttatc cggcctacgg ttcggcacag acttgtaggc atgataagac gcgtcagcgt
    42061 cgcatcaggc attgtgcgcc aactgccgga tgcggcgtga acgccttatc cggcctacgg
    42121 ttcggcacag ac
//
LOCUS       DAZPLI010000020        41011 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZPLI010000020 DAZPLI010000000
VERSION     DAZPLI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41011
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.020"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..262
                     /locus_tag="ACHUU3_001011"
     CDS             <1..262
                     /locus_tag="ACHUU3_001011"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2081157.1"
                     /translation="PVKYNHSIKPAKNYRFINKYILTPAPFSPIDNHHYVFYFFTAKS
                     LAHSAALRHFFKQANTTSNKYQVNGEGYVFNFWRIRYQNRRS"
     gene            216..1880
                     /gene="asnB"
                     /locus_tag="ACHUU3_001012"
     CDS             216..1880
                     /gene="asnB"
                     /locus_tag="ACHUU3_001012"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR2081158.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            2260..2336
                     /locus_tag="ACHUU3_001013"
     tRNA            2260..2336
                     /locus_tag="ACHUU3_001013"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2294..2296,aa:Met,seq:cat)
     gene            2345..2429
                     /locus_tag="ACHUU3_001014"
     tRNA            2345..2429
                     /locus_tag="ACHUU3_001014"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2379..2381,aa:Leu,seq:tag)
     gene            2453..2527
                     /locus_tag="ACHUU3_001015"
     tRNA            2453..2527
                     /locus_tag="ACHUU3_001015"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2485..2487,aa:Gln,seq:ttg)
     gene            2562..2636
                     /locus_tag="ACHUU3_001016"
     tRNA            2562..2636
                     /locus_tag="ACHUU3_001016"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2594..2596,aa:Gln,seq:ttg)
     gene            2652..2728
                     /locus_tag="ACHUU3_001017"
     tRNA            2652..2728
                     /locus_tag="ACHUU3_001017"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2686..2688,aa:Met,seq:cat)
     gene            2777..2851
                     /locus_tag="ACHUU3_001018"
     tRNA            2777..2851
                     /locus_tag="ACHUU3_001018"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2809..2811,aa:Gln,seq:ctg)
     gene            2888..2962
                     /locus_tag="ACHUU3_001019"
     tRNA            2888..2962
                     /locus_tag="ACHUU3_001019"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2920..2922,aa:Gln,seq:ctg)
     gene            complement(3116..4291)
                     /gene="ubiF"
                     /locus_tag="ACHUU3_001020"
     CDS             complement(3116..4291)
                     /gene="ubiF"
                     /locus_tag="ACHUU3_001020"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR2081159.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            4437..5861
                     /gene="miaB"
                     /locus_tag="ACHUU3_001021"
     CDS             4437..5861
                     /gene="miaB"
                     /locus_tag="ACHUU3_001021"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR2081160.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            6014..7054
                     /locus_tag="ACHUU3_001022"
     CDS             6014..7054
                     /locus_tag="ACHUU3_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR2081161.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            7051..7518
                     /gene="ybeY"
                     /locus_tag="ACHUU3_001023"
     CDS             7051..7518
                     /gene="ybeY"
                     /locus_tag="ACHUU3_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR2081162.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            7608..8486
                     /gene="corC"
                     /locus_tag="ACHUU3_001024"
     CDS             7608..8486
                     /gene="corC"
                     /locus_tag="ACHUU3_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR2081163.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            8511..10049
                     /gene="lnt"
                     /locus_tag="ACHUU3_001025"
     CDS             8511..10049
                     /gene="lnt"
                     /locus_tag="ACHUU3_001025"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR2081164.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(10127..12004)
                     /locus_tag="ACHUU3_001026"
     CDS             complement(10127..12004)
                     /locus_tag="ACHUU3_001026"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFR2081165.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            12459..13367
                     /gene="gltI"
                     /locus_tag="ACHUU3_001027"
     CDS             12459..13367
                     /gene="gltI"
                     /locus_tag="ACHUU3_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR2081166.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            13537..14277
                     /gene="gltJ"
                     /locus_tag="ACHUU3_001028"
     CDS             13537..14277
                     /gene="gltJ"
                     /locus_tag="ACHUU3_001028"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR2081167.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            14277..14951
                     /gene="gltK"
                     /locus_tag="ACHUU3_001029"
     CDS             14277..14951
                     /gene="gltK"
                     /locus_tag="ACHUU3_001029"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR2081168.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            14951..15676
                     /gene="gltL"
                     /locus_tag="ACHUU3_001030"
     CDS             14951..15676
                     /gene="gltL"
                     /locus_tag="ACHUU3_001030"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR2081169.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            15794..16729
                     /gene="rihA"
                     /locus_tag="ACHUU3_001031"
     CDS             15794..16729
                     /gene="rihA"
                     /locus_tag="ACHUU3_001031"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR2081170.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            16821..18491
                     /gene="hscC"
                     /locus_tag="ACHUU3_001032"
     CDS             16821..18491
                     /gene="hscC"
                     /locus_tag="ACHUU3_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFR2081171.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(18551..20002)
                     /gene="djlC"
                     /locus_tag="ACHUU3_001033"
     CDS             complement(18551..20002)
                     /gene="djlC"
                     /locus_tag="ACHUU3_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR2081172.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(19999..20706)
                     /locus_tag="ACHUU3_001034"
     CDS             complement(19999..20706)
                     /locus_tag="ACHUU3_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR2081173.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            20808..21362
                     /locus_tag="ACHUU3_001035"
     CDS             20808..21362
                     /locus_tag="ACHUU3_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR2081174.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(21372..22799)
                     /locus_tag="ACHUU3_001036"
     CDS             complement(21372..22799)
                     /locus_tag="ACHUU3_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR2081175.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(22796..23503)
                     /locus_tag="ACHUU3_001037"
     CDS             complement(22796..23503)
                     /locus_tag="ACHUU3_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR2081176.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            23667..24644
                     /gene="ybeQ"
                     /locus_tag="ACHUU3_001038"
     CDS             23667..24644
                     /gene="ybeQ"
                     /locus_tag="ACHUU3_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFR2081177.1"
                     /translation="MVFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFMKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(24714..25196)
                     /locus_tag="ACHUU3_001039"
     CDS             complement(24714..25196)
                     /locus_tag="ACHUU3_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR2081178.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            25431..28013
                     /gene="leuS"
                     /locus_tag="ACHUU3_001040"
     CDS             25431..28013
                     /gene="leuS"
                     /locus_tag="ACHUU3_001040"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR2081179.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            28028..28609
                     /gene="lptE"
                     /locus_tag="ACHUU3_001041"
     CDS             28028..28609
                     /gene="lptE"
                     /locus_tag="ACHUU3_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR2081180.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            28609..29640
                     /gene="holA"
                     /locus_tag="ACHUU3_001042"
     CDS             28609..29640
                     /gene="holA"
                     /locus_tag="ACHUU3_001042"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR2081181.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            29642..30283
                     /gene="nadD"
                     /locus_tag="ACHUU3_001043"
     CDS             29642..30283
                     /gene="nadD"
                     /locus_tag="ACHUU3_001043"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR2081182.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            30307..30918
                     /locus_tag="ACHUU3_001044"
                     /pseudo
     CDS             30307..30918
                     /locus_tag="ACHUU3_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            31178..31495
                     /gene="rsfS"
                     /locus_tag="ACHUU3_001045"
     CDS             31178..31495
                     /gene="rsfS"
                     /locus_tag="ACHUU3_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR2081183.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            31499..31966
                     /gene="rlmH"
                     /locus_tag="ACHUU3_001046"
     CDS             31499..31966
                     /gene="rlmH"
                     /locus_tag="ACHUU3_001046"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR2081184.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            31997..33898
                     /gene="mrdA"
                     /locus_tag="ACHUU3_001047"
     CDS             31997..33898
                     /gene="mrdA"
                     /locus_tag="ACHUU3_001047"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR2081185.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            33901..35013
                     /gene="mrdB"
                     /locus_tag="ACHUU3_001048"
                     /gene_synonym="rodA"
     CDS             33901..35013
                     /gene="mrdB"
                     /locus_tag="ACHUU3_001048"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR2081186.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            35024..36112
                     /gene="rlpA"
                     /locus_tag="ACHUU3_001049"
     CDS             35024..36112
                     /gene="rlpA"
                     /locus_tag="ACHUU3_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR2081187.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            36251..37462
                     /gene="dacA"
                     /locus_tag="ACHUU3_001050"
     CDS             36251..37462
                     /gene="dacA"
                     /locus_tag="ACHUU3_001050"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR2081188.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            37572..37835
                     /gene="ybeD"
                     /locus_tag="ACHUU3_001051"
     CDS             37572..37835
                     /gene="ybeD"
                     /locus_tag="ACHUU3_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR2081189.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            37936..38577
                     /gene="lipB"
                     /locus_tag="ACHUU3_001052"
     CDS             37936..38577
                     /gene="lipB"
                     /locus_tag="ACHUU3_001052"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR2081190.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            38837..39790
                     /locus_tag="ACHUU3_001053"
     CDS             38837..39790
                     /locus_tag="ACHUU3_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR2081191.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            39999..40964
                     /gene="lipA"
                     /locus_tag="ACHUU3_001054"
     CDS             39999..40964
                     /gene="lipA"
                     /locus_tag="ACHUU3_001054"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR2081192.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT        10296 a        10448 c        10293 g         9974 t
ORIGIN      
        1 ccccgtaaaa tataaccatt caataaaacc cgctaaaaat tatcgattca tcaataaata
       61 catcctcaca ccagcccctt tttcaccaat cgacaatcac cattacgttt tttatttttt
      121 caccgccaaa tcgcttgcgc acagtgctgc tttgcggcat ttttttaaac aagcaaacac
      181 aacaagcaac aaataccagg ttaacggaga aggttatgtg ttcaattttt ggcgtattcg
      241 atatcaaaac agacgcagtt gagcagcgta agaaagcact cgagctgtca cgcctgatgc
      301 gtcatcgtgg cccggactgg tccggtattt atgccagcga taacgccatt ctcgctcacg
      361 aacgtctgtc aattgttgac gttaacgcgg gggcgcaacc tctctacaac caacaaaaaa
      421 cccacgtact ggcggtaaac ggtgaaatct acaaccacca ggcattgcgc gctgaatatg
      481 gcgatcgtta tcagttccag acagggtctg actgtgaagt gatcctcgcg ctgtatcagg
      541 aaaaagggcc ggaatttctc gacgacttgc agggcatgtt tgcctttgcc ctgtacgaca
      601 gcgaaaaaga tgcctacctg attggtcgcg accatctggg gatcatccca ctgtatatgg
      661 ggtatgacga acacggtcag ctgtatgtgg cctcagaaat gaaatcgctg gtgccagttt
      721 gccgcacgat taaagagttc ccggcgggga gctatttgtg gagccaggac ggcgaaatcc
      781 gttcttatta tcatcgcgac tggttcgact ttgatgcagt gaaagataac gtgaccgaca
      841 aaaacgagct gcgtcaggct ctggaagatt ccgttaaaag ccatctgatg tctgatgtgc
      901 cttacggtgt gctgctttct ggtggtctgg attcctcaat tatttccgct atcaccaaga
      961 aatacgcagc ccgtcgcgtg gaagatcagg aacgctctga agcctggtgg ccccagttac
     1021 actcctttgc tgtaggtctg ccgggttcac cggatcttaa agcagcccag gaagtggcaa
     1081 accatctggg cacggtgcat cacgaaattc acttcactgt acaggaaggt ctggatgcca
     1141 tccgcgacgt gatttaccac atcgaaactt atgatgtgac cactattcgc gcttcaacac
     1201 cgatgtattt aatgtcgcgt aagatcaagg cgatgggcat taaaatggtg ctgtccggtg
     1261 aaggttctga tgaagtgttt ggcggttatc tttacttcca taaagcgccc aacgctaaag
     1321 aactgcatga agagacggtg cgtaaactgc tggccctgca tatgtatgac tgcgcgcgcg
     1381 ccaacaaagc gatgtcagcc tggggcgtgg aagcacgcgt tccgttcctc gacaaaaaat
     1441 tccttgatgt ggcgatgcgc attaacccgc aggataaaat gtgcggtaac ggcaaaatgg
     1501 aaaaacacat cctgcgtgaa tgttttgagt catacctgcc cgcaagcgtg gcctggcggc
     1561 agaaagagca gttctccgat ggcgtcggtt acagttggat cgacaccctg aaagaagtgg
     1621 cggcgcagca ggtttctgat cagcaactgg aaactgcccg cttccgcttc ccgtacaaca
     1681 cgccaacctc aaaagaagcg tatctgtacc gggagatctt tgaagaactg ttcccgcttc
     1741 cgagcgccgc tgagtgcgtg ccgggcggcc cgtccgtcgc gtgttcttcc gctaaagcga
     1801 ttgagtggga tgaggcgttc aagaaaatgg acgatccgtc tggtcgtgcg gttggcgttc
     1861 accagtcggc gtataagtaa gaaatgcgaa acagccccgg tgaaatccgg ggctgtttca
     1921 gttattgagg tgcttgttga ttctgatatg cagtaaggaa atgttccggg tttgccacat
     1981 cactgtttga caactcatca ctgtaggcca gtatttcata gattaatttc ccaccaccat
     2041 tgttgaattc cgaatttcat cctgtatgac aaaagaatgg ctgtagggtg gggaaaaaac
     2101 acaaagtaaa caataattga cgaatatagc gccacgctgt tcgcaaccta accaaacagt
     2161 cactttcgag caattttcct tgaaaaagag gttgacgctg caaggctcta tacgcataat
     2221 gcgccccgca acgccgataa ggtatcgcga aaaaaagatg gctacgtagc tcagttggtt
     2281 agagcacatc actcataatg atggggtcac aggttcgaat cccgtcgtag ccaccatctt
     2341 ttttgcggga gtggcgaaat tggtagacgc accagattta ggttctggcg ccgcaaggtg
     2401 tgcgagttca agtctcgcct cccgcaccat tcaccagaaa gcgttgtacg gatggggtat
     2461 cgccaagcgg taaggcaccg gtttttgata ccggcattcc ctggttcgaa tccaggtacc
     2521 ccagccatct tcttcgagta agcggttcac cgaccggtta ttggggtatc gccaagcggt
     2581 aaggcaccgg tttttgatac cggcattccc tggttcgaat ccaggtaccc cagccatcga
     2641 agaaacaatc tggctacgta gctcagttgg ttagagcaca tcactcataa tgatggggtc
     2701 acaggttcga atcccgtcgt agccaccaaa ttctgaatgt gtcgaatacg ttcggcaaat
     2761 tcaaaaacca atttgttggg gtatcgccaa gcggtaaggc accggattct gattccggca
     2821 ttccgaggtt cgaatcctcg taccccagcc aatttattca agacgcttac atcgtaagtg
     2881 caccagttgg ggtatcgcca agcggtaagg caccggattc tgattccggc attccgaggt
     2941 tcgaatcctc gtaccccagc cacattaaaa aagctcgctt cggcgagttt tttgcttttc
     3001 tgcgttcatt caatgtcgaa tgcgatgttg acacgtctta tccttcaatg tcggatgcga
     3061 cgctgccgcg tcttatccga cctacggttg gcacgcatcc ggcaatgttg taaggctaca
     3121 accctaacgc atatttcagc gcctgacgtt tcaacacgcc agcacgctct gccgccatta
     3181 acccgagatt acgcacaaaa cgcagcggcg gcagattatt actgaatccg gcataaaaca
     3241 gatccatacc actttgcata atgaagttat ccgccatgcg ccgcatctgg taacgcttga
     3301 ggacaggata actggcccac gcttcaccgt agctgcgggc attcaccaga acatcaatca
     3361 gggcatcgac atcacgataa ccaagattca ccccctgccc cgccagcgga tggatggtat
     3421 gcgcggcatc gcccaccagc gcaagccccg gctgcacata ctgcaacgca tgtcggcgcg
     3481 tcagcggaaa cgcaccagcg gcaagcggtg taacgtaacc cagacgcgac gggaaatgct
     3541 tcgcgatttc cgcctggaat tgcgccatat tcatattctg caactggcga atacgcgccg
     3601 gagagtcata ccacaccagc gatgcccagt tatcaaacaa cggcagaaac gcacgcggtc
     3661 cgtccggggt aaattgctgc caggtgctgt cgccgggatc gttctcacac tgtacgctaa
     3721 tcaacatgca cgactgcgca tactgccagg catgaacgcc aattcccgcc atctgccgca
     3781 cctgagaatt tgcgccgtcg gcaccaatca ccagtttcgc gcggatcgtt tcaccgccct
     3841 tcagttccag ctcctgaaga tcattatctc gatgtaatgc aatcagcgag cctggcacac
     3901 gtaacgttac tttcggatgc gcttccagcg cctgccacag cgcctgttgc aggacggtgt
     3961 tttccaccat atagccaagc aacggtagct taagttcagc ggcgtcaaac accacatgcg
     4021 ccgtttccca ctcccacgtt tccagtctgc ggtaaggatg gcaacgcata gcctgtactg
     4081 catcccagac ccctaaccct ttaagcaatg ataccgatgc ggcgctgatc gccgagatcc
     4141 gcacgtccgg ttggctatca gcgacaaacg gtgcaggttc tgcgtgctcg atcaccgtta
     4201 ccgaaaatcc gtgctgtgcc agccccagcg ccagcgcgcc gccgaccatt cctccgccga
     4261 caatggcaat ttccgttggt tgatttgtca tggtcaatca tcctgttagc aatatgcttt
     4321 aagtttacag gatttttacc cccaagggct gataacgctc atactggtca caacggcagc
     4381 aaagcattac actatacgcc ctgcattcct ggctactatt tcgcaagagc aagtcgatga
     4441 ccaaaaaact ccatattaaa acctggggct gtcagatgaa cgagtacgat tcatcgaaga
     4501 tggccgatct gctggatgcc acccacggct atcaactgac cgacgtggcg gaagaagcgg
     4561 atgtgctgct gctgaacacc tgctcaatcc gcgagaaggc tcaggaaaaa gtcttccatc
     4621 agttgggtcg ctggaaactg ttaaaagaga agaatccaga cctgattatc ggcgtcggtg
     4681 gctgcgtggc atcgcaagaa ggcgagcaca ttcgccagcg cgcccactat gtcgatatta
     4741 tttttgggcc gcaaacgctg caccgtctgc cggagatgat caactccgtg cgcggcgacc
     4801 gcagcccggt tgtagatatc agcttcccgg aaatcgagaa gtttgaccgt ctaccggaac
     4861 cgcgcgccga agggccgacc gcgtttgtct ccatcatgga aggctgcaat aaatattgca
     4921 cctactgcgt ggtgccttac acccgtggtg aagaggtaag ccgtccgtct gacgatattc
     4981 tgtttgagat tgcccagctt gcggctcagg gcgtgcgtga agtcaacctg ctcggtcaga
     5041 acgtgaacgc ctggcgtggt gaaaactacg acggctccac cggatcgttt gccgatctgc
     5101 tgcgtctggt tgctgcgatc gacgggatcg atcgtattcg ctttaccacc agccatccga
     5161 tcgaattcac cgacgatatc atcgaagtgt atcgcgacac gccggagctg gtgagcttcc
     5221 tgcatctgcc ggtacaaagc ggttccgatc gcattctgaa cctgatgggc cgtactcaca
     5281 cggcgctgga gtacaaagcg atcatccgta aactgcgtgc ggcgcgtccg gatattcaga
     5341 tcagttctga cttcattgtt ggcttccctg gcgaaaccac cgaagacttc gagaaaacga
     5401 tgaagctgat tgccgacgtc aatttcgaca tgagctacag ctttatcttc tctgcacgtc
     5461 cgggtacacc agccgccgat atggttgatg atgttccgga agaagagaag aagcaacgtc
     5521 tgtatattct gcaagaacgt atcaatcagc aagcgatggc atggagccgc cggatgctcg
     5581 gcaccaccca gcgtattctg gtagaaggga cttcgcgtaa gagcatcatg gagctttccg
     5641 ggcgtaccga aaataaccgc gtggtcaact tcgagggcac accggatatg atcggtaaat
     5701 tcgtcgatgt agaaattacc gacgtctacc cgaactctct gcgcggtaaa gtggtgcgta
     5761 ctgaagatga aatgggtctg cgcgtggcag aaacaccgga atcggtgatt gcccgtaccc
     5821 gcaaagaaaa cgaccttggc gtgggttatt atcagccgta attctcaggc ccgccgttcc
     5881 ggtgggcctt gttttttctc tctttatccc catctttatc gcaatgcttg cgcctttttc
     5941 ctgtggcgtg aataatttcc ttataggccg gtgaatgttc agcttccggc ggcatacaga
     6001 taagaggaac ggtttgaaca tagacactcg cgaaatcacc ctggagccag cagacaacgc
     6061 gcgtctgttg agcctgtgcg gcccgtttga tgacaacatc aagcagctcg aacgccgtct
     6121 cggcatcgag atcaatcgcc gcgacaacca ctttaaactg accggccgtc cgatttgcgt
     6181 caccgctgcg gcagacattc tgcgtagcct gtatgtcgat acagccccga tgcgcggtca
     6241 gattcaggat atcgaaccgg aacagatcca ccttgcgatt aaagaagcgc gagtactgga
     6301 gcaaagtgcg gagagcgtgc cggagtacgg caaagcggtc aatatcaaaa ccaaacgcgg
     6361 cgtaattaag ccgcgcacgc caaaccaggc gcagtacatc gccaatattc tcgaccatga
     6421 cattaccttc ggcgttggcc cggcgggtac gggtaaaacc tacctggcag tggctgcggc
     6481 agttgatgcc ctggagcgtc aggagattcg ccgtattctg ctgactcgtc cggcggtcga
     6541 agccggtgag aaactgggct tcctgcctgg cgatttaagc cagaaagtag acccgtatct
     6601 gcgcccactg tacgacgcgc tgtttgaaat gctgggcttt gagaaagttg agaaactgat
     6661 tgagcgcaac gttattgaag tcgcgccgct ggcctatatg cgtggtcgta cgctgaacga
     6721 cgcgtttatc attctcgatg agagccagaa caccaccatc gaacagatga agatgttcct
     6781 gacccgtatc ggttttaact caaaagcggt tatcaccggc gacgtcacac agatcgacct
     6841 gccgcgtaat actaaatcag gcttacgtca cgccatcgaa gtgctggccg atgtcgaaga
     6901 gatcagcttt aacttcttcc acagcgaaga cgtggttcgt caccctgtgg tggcgcgtat
     6961 cgttaacgcc tatgaagcct gggaagaagc cgaacaaaaa cgaaaagcgg cgctggcggc
     7021 agaacgcaag cgcgaagaac aggaacaaaa atgagtcagg tgatcctcga tttacaactg
     7081 gcatgtgaag ataattccgg gttaccggaa gagagccagt ttcaaacatg gctgaatgcg
     7141 gtgatcccgc agtttcagga agaatcggaa gtgactattc gcgtggtcga taccgccgaa
     7201 agccacagtc tgaatctgac ctatcgcggt aaggataagc cgaccaacgt gctctccttc
     7261 ccgtttgaag tgccgcctgg catggaaatg tcgctactgg gcgatctggt tatctgccgt
     7321 caggtggttg agaaggaagc tcaggagcaa ggcaaaccac tggaggcgca ctgggcgcat
     7381 atggtggtgc acggcagtct gcatttgtta ggttacgatc acatcgaaga tgacgaagca
     7441 gaagaaatgg aagccctcga aacagagatt atgcttgctc tgggctatga ggatccgtac
     7501 attgccgaga aagaataatt tgccaggcat ttgactggca gacgtccccg ccgttggtga
     7561 ttaacgacgg cgttttgatt aactaacatg agaacccata cgacgccatg agcgacgaca
     7621 attcacacag tagtgacacg ataagcaaca agaagggatt tttctccctg ttactcagcc
     7681 aacttttcca cggtgaaccg aaaaaccgtg acgaactgct ggcgctgatc cgtgattccg
     7741 ggcagaacga ccttatcgac gaagatacgc gcgatatgct cgaaggggtg atggacatcg
     7801 cagaccaacg cgttcgcgac atcatgatcc cccgctccca gatgattacc ctgaaacgca
     7861 accagacgct ggacgaatgc cttgatgtca tcatcgagtc cgcccactca cgtttcccgg
     7921 tgattagcga agacaaagat cacattgaag ggattctgat ggcgaaagac ttgctgccgt
     7981 ttatgcgcag cgatgctgaa gccttcagca tggacaaagt gttacgtcag gcggttgtcg
     8041 ttcctgaaag taagcgcgta gaccggatgc tgaaagagtt tcgctctcag cgttaccaca
     8101 tggcgatcgt cattgacgaa ttcggtgggg tttccggtct ggtgaccatt gaagacatcc
     8161 tggaactgat tgttggtgag attgaagacg agtatgacga agaagatgat atcgacttcc
     8221 gtcagctgag tcgtcatacc tggaccgtgc gtgcactggc ttccattgaa gacttcaacg
     8281 aagcgttcgg cacccacttt agcgatgaag aggttgacac tatcggtggt ctggtgatgc
     8341 aggcatttgg gcatcttccg gcgcgtggcg aaactatcga catcgacggt taccagttca
     8401 aagtggcgat ggccgacagt cggcgtatta ttcaggttca tgtcaaaatc ccggatgact
     8461 caccccagcc gaagctggat gaataaaacc gaaactggat agataactac atggcttttg
     8521 cctcattaat tgaacgccag cgcattcgcc tgctgctggc gttattattc ggtgcctgcg
     8581 gaacgctggc cttctctcct tacgacgtct ggcctgcggc gattatttcg ttgatggggc
     8641 ttcaggcgtt gacctttaac cgccgtccac tccagtctgc cgctattggc ttttgctggg
     8701 gatttggcct ctttggcagc ggtattaact gggtctatgt cagcatcgcg acctttggcg
     8761 gaatgcctgg cccggttaac atcttcctgg tggtgctgct ggcggcgtat ttgtcgctgt
     8821 ataccggact gtttgctggc gtgctgtcgc gtctgtggcc gaaaaccacc tggctgcgcg
     8881 tggcgattgc cgcccctgct ctctggcaag tgaccgagtt tctgcgcggt tgggtactga
     8941 caggcttccc gtggttacag ttcggctata gccagattga tggcccgtta aaagggctgg
     9001 caccgataat gggcgtggaa gccattaact tcctgctgat gatggttagt ggcctgctgg
     9061 cactggcgtt ggtcaaacgc aactggcgtc cgctggtggt ggccgtcgtg ctgtttgccc
     9121 tacccttccc gctgcgttac atccaatggt ttaccccgca accggagaaa accattcagg
     9181 tttcgatggt tcagggcgat attccgcaat cactgaaatg ggatggagat cagcttctta
     9241 atacgctgaa gatttactac aacgcaacgg ctccgctgat gggcaaatca tcgttgatta
     9301 tctggccgga gtcggcgata accgatctgg aaattaatca gcaaccgttc ctcaaagcac
     9361 tggacggtga gttgcgcgat aaaggtagct cgctggtaac cgggattgtc gacgcgcgtc
     9421 tcaataagca gaaccgctac gatacctaca acaccatcat cacactgggt aaaggtgcac
     9481 catacagcta cgaatcagcc gatcgctata acaaaaacca tctagtgccg tttggcgagt
     9541 ttgtcccgct ggagtcgatt ctgcgtccgt tagcaccgtt ctttgatctg ccgatgtcgt
     9601 cgttcagccg tgggccatat atccagccgc cgctgtcggt aaatggtatt gagcttactg
     9661 cggctatttg ctacgagatc attctcggcg agcaagtgcg cgataacttc cgcccggata
     9721 ccgactatct gctgactatc tccaacgatg cgtggtttgg taagtctatt ggtccatggc
     9781 agcacttcca gatggcgcga atgcgtgcgc tggagctggc gcgcccactg ttgcgcagca
     9841 ccaacaacgg cattacggcg gtgattggcc cgcaggggga ggttcaggcg atgatcccgc
     9901 agttcacccg cgaggtgttg accactaatg tgacaccgac caccggactc acaccgtatg
     9961 cacgtaccgg caactggcca ctgtgggtgc tgacggcgct gtttggtttt gccgctgtgt
    10021 tgatgagtct gcgagcacgt aaacgttgat gtgcctgatg tatcctgcat gagcaaaacg
    10081 tactttctca ttgagctttg gggcatggtt atcatgcccc tttttcttac ggttcaggga
    10141 tacaagccac attgccatat tggggatgac gaataattgc ctgcggatca agcgagcgcg
    10201 tcctgtccat ttggatcaac ccgttcttcg ccatgacaaa tatttttcta ccatccgctg
    10261 aaaaacgaag gtgattaacg tagctggcag gataaacgcc aataatctgc ccggcattaa
    10321 tattgagcag gatgacataa ccttgcaggc catatgacac caacgctaaa tattgaccgt
    10381 ccgggctaat accctcaacg gaataaacgc tcctgctaca ccattgcgtc ataatgctgg
    10441 cttgttgatc ccctacgctt atacgtgaaa tcccctgact atcggcaaaa ataacctcgc
    10501 cgttttcagg tcgaaaacgc atcgccgata tcgttccacg cgcatcacct gcgggaaagt
    10561 cttctttctg atgcgtgatg gtattgatcc gcacgatgtt acctctgcct gctcccggaa
    10621 ccagtaatgt tctttcatcc ggggaaagtg ccataaccca ggcgcctggt ttaccgctcg
    10681 tccccgtccc gtcgtatttc tccagcggga tatccgccac atgctgataa gtcaccagat
    10741 caatcgctga cacactatta ctggtgcgat tcatcaccca ggcttttgtg ccatcacgcg
    10801 agaggataag gctgtcagga tgtgcaccgg tggtaatcga ctgaataatc tcccctgtcg
    10861 ccacgttaat gaccgataac gcattcgctt caaatgaagt cgcataaatc aacttttcat
    10921 ccgggcttaa tgccagcgat ctgacgccct gatctttaca gccaccacaa tgtttgactg
    10981 acttttcttt cgctatgggc gctgtaaagg tatgcaggat tttcttttgt ccgaggtcga
    11041 aaacaacaat gctgttcgtg ttttcaattg cggcatacaa catattacca tcccgactga
    11101 tcgccatatc atggatatcg gcaatctcag cgagcaaatc accactggcc tgttcacgac
    11161 ttaccggagc ggataatgtt tccctgaggg cgttgcgttc ctccgctaac tgctgctttt
    11221 tttgccgtcg ctcttgttca atctcctgtg gataaaacgc ttcacgcagg ctttgcctga
    11281 cctgtaaaac tgactcatcc atctgctgct gcgcaagcca gatagccccg gtcagaagaa
    11341 gactgcccgc aacaataatc ccgccttccg taacgagtcg gttttgccca gataaacgcg
    11401 cgctcagcca acccaacgcg ccacccgcaa tcaggccgcc aatgtggcaa gcgttatcta
    11461 ttcctgtttg ccgggcaccg tacaacagtg tcagcgccac catacccaac agaggaaata
    11521 ctcgccgctg gtttttatgg tatgtgcccg tacctgaagc aaactgtgtt gctatcgctg
    11581 caccagctat tcccagaatc gccccggatg ccccaacgct gatgactatc tgctcacttt
    11641 cgcgtaacgc ataacaggcg ctgatgagcc caccaaagac gccggagaat aaccaggtaa
    11701 tcaggagtcg ccatttcccc agaatctgtt ccgccacgcc tcccacgacc aggaatgcaa
    11761 acatattcat cagcagatga gaaacactac tgtgcagaaa gatactgctc aataagcgcc
    11821 agggttcacc cgacaaagta agaggcgcaa cgtttgcgcc gagatggata agtgcggatt
    11881 gatcgacctc ttcataactc atccattgtg cgagataaca aaatatccca atacagagtg
    11941 caaaaaggat aagggttatt gcgggtaatt gaacatttaa cggcttaacc gaagatgcag
    12001 acataaggtg tttcttcctt gaaaagacag gtaaaaagca tttttcccgt gtgcagttat
    12061 catcccggca aacgtcccat gccgaaaaga taattctgtc cgaaatatga ctttttcagc
    12121 ctaaaattac aatcgtaatt aattcgcccc taagtatttt ttggcatgta agttgcttta
    12181 tttactgtgt aagcgatgaa atcagccgcg acgcaaccag gttgcaccac cacaaatcag
    12241 cggtagcacc aaaaaggtgc ggtttaagaa ttttgcttcg gaatggtgcg gcgcatctcg
    12301 caaaaacaaa caataaaaca gcaatatctt tacatttagc caaactaaat gttacctaac
    12361 aggcacaaca ctgcacaata aagttgcaga cgataacaac acaaacactc acaacgggta
    12421 tccatgcgtt cttaacgcag aagataaagg agttggatat gcaattacgt aaacctgcca
    12481 cagcaatcct cgccctggcg ctttccgcag gactggcaca ggcagatgac gccgccccgg
    12541 cagcaggcag tacgctggac aaaatcgcca aaaacggtgt gattgtcgtc ggtcaccgtg
    12601 aatcttcagt acctttctct tattacgaca atcagcaaaa agtggtgggt tactcgcagg
    12661 attactccaa cgccattgtt gaagcagtga aaaagaaact caacaaaccg gacttgcagg
    12721 taaaactgat tccgattacc tcacaaaacc gtattccact gctgcaaaac ggcactttcg
    12781 attttgaatg tggttctacc accaacaacg tcgaacgcca aaaacaggcg gctttctctg
    12841 atactatttt cgtggtcggt acgcgcctgc tgaccaaaaa gggcggcgat atcaaagatt
    12901 ttgccgacct gaaagacaaa gccgtagtcg tcacttccgg cactacctct gaagttttgc
    12961 tcaacaaact gaatgaagag caaaaaatga atatgcgcat catcagcgcc aaagatcaca
    13021 gtgactcttt ccgcaccctg gaaagcggtc gtgccgttgc ctttatgatg gatgatgccc
    13081 tgctggccgg tgaacgtgcg aaagcgaaga aaccagacaa ctgggaaatc gtcggcaagc
    13141 cgcagtctca ggaagcctac ggttgcatgt tgcgtaaaga cgatccgcag ttcaaaaagc
    13201 tgatggatga caccaccgcg caggtgcaga cctccggtga agcggaaaaa tggtttgata
    13261 aatggttcaa aaatcccatt ccgccgaaaa acctgaacat gaatttcgaa ctgtcagacg
    13321 aaatgaaagc actgttcaaa gaaccgaatg acaaggcact gaactaatta caagaaccag
    13381 gggcggaaat tccagccctc tcgattgtta cgtagcacgg acagactata cgcctgatgg
    13441 tcgttcccca tcgggcctga aaaccgcaat acgctgggta acaatcttcg agggtagcag
    13501 ttaacgctgc taccctcttt tttctggagt agatttatgt ctatagactg gaactggggt
    13561 atttttttac aacaagcccc gttcggcaac accacctatc ttggttggat ctggagtggt
    13621 tttcaggtga cgatcgcttt gtcgatctgc gcctggatta tcgctttcct cgtcggttca
    13681 tttttcggca ttttacgtac cgttccaaac cgttttctct ccggtctggg tacgttgtat
    13741 gtcgaactgt tccgcaacgt gccgttgatt gtgcaattct ttacctggta tctggtgatc
    13801 ccggagctgt tgccggagaa aatcggcatg tggtttaagg ccgagctgga tcccaatatt
    13861 cagtttttcc tttcatccat gctctgcctg gggctgttta ccgccgcccg tgtttgcgaa
    13921 caggtacgcg ccgccattca gtcgttgccg cgtgggcaaa aaaatgccgc gctggcgatg
    13981 ggcctgacgc taccgcaggc ttatcgatac gtactgctgc ctaatgctta tcgcgttatc
    14041 gtcccgccga tgacttccga gatgatgaac ctggtgaaaa actccgccat cgcctcgact
    14101 attggtctgg tggatatggc ggcgcaggcg ggtaaattgc ttgattactc agcccacgcc
    14161 tgggagtcgt ttaccgctat tacgctggcg tatgtgttga ttaacgcggt tatcatgctg
    14221 gtaatgacgc tggttgaacg taaagttcgc ctgcctggca acatgggggg caaataatgt
    14281 acgagtttga ctggagttcc attgtccctt ccctgccata tctgctcgac gggctggtga
    14341 tcacgctgaa aatcaccgtc acggcagtcg tgattggtat tttgtggggc accatgctgg
    14401 cggtgatgcg cttgtccagc tttgcgcccg tagcgtggtt tgccaaagcc tacgttaacg
    14461 tattccgctc gattccttta gttatggttt tgctgtggtt ttacctgatc gtgccaggtt
    14521 ttctgcaaaa cgtgctggga ttatcaccaa aaaatgatat ccgtttaatt tcggcgatgg
    14581 tggcgttttc gatgtttgaa gcggcctact attcagagat tatccgtgct ggtattcaaa
    14641 gtatttctcg tggtcagtcg agcgccgcgc tggcgttggg aatgactcac tggcagtcga
    14701 tgaaactgat tattctgccg caggcgttcc gcgcgatggt gccgcttctg ctcactcagg
    14761 gcatcgtact gttccaggat acctcactgg tgtatgtgtt atctctggcc gatttcttcc
    14821 gtaccgcctc aaccattggt gagcgtgatg gtacgcaggt tgagatgatc ctgtttgccg
    14881 gatttgttta tttcgttatt agtcttagcg cgtcgttgtt ggtcagctac ttgaaaagaa
    14941 ggacagcata atgattaccc tgaaaaatgt ttcaaaatgg tatggtcact ttcaggtgct
    15001 gaccgactgc tcaaccgaag tgaaaaaagg cgaagtggtg gtggtttgcg gcccgtctgg
    15061 ttccggcaaa tcaacgctga ttaaaaccgt caacggcctc gaaccagtgc agcaaggtga
    15121 aatcaccgtc gatggtatcg tggttaatga caagaaaacc gatctggcaa agctgcgttc
    15181 ccgcgtcggg atggtattcc agcatttcga gctgttccct catctgtcga ttatcgaaaa
    15241 cctgaccctg gcgcaggtga aagtgcttaa acgcgataaa gcgcccgctc gtgaaaaagc
    15301 attgaaattg ctggagcgcg tgggactttc cgcacatgcc aataaatttc cggcgcagct
    15361 ttccggcggt cagcagcagc gtgtggcaat cgctcgcgca ttgtgtatgg atcctattgc
    15421 gatgctgttc gacgaaccga catcggcgct ggatccggag atgatcaacg aagtgctgga
    15481 cgtgatggtg gaactggcga acgaaggaat gaccatgatg gtggtgaccc acgaaatggg
    15541 ctttgcccgc aaagtggcga atcgggtgat ctttatggac gagggtaaaa ttgtcgaaga
    15601 ctcgccgaaa gacgctttct tcgatgatcc gaaatcggac cgcgcgaaag acttcctcgc
    15661 gaaaatcctg cattaatcac tctggcgcgc atacccaagt gcgcgccatc tcacgcttta
    15721 tcgtttcgcc cctcgtatct gttacatcct ggcgttaccc ttgtcgcaaa gaagcacaca
    15781 acaaggagca acaatggcac tgccaattct gttagattgc gacccaggtc atgacgacgc
    15841 tatcgcaata gttctcgccc tcgcctcacc agagcttgat gtcaaagcaa ttacgtcttc
    15901 cgccggaaac cagacaccag aaaaaacctt acgcaatgtt ctgcgtatgc tgaccttgct
    15961 taatcgcacc gatattccgg tagcaggcgg cgcggtaaaa ccgttaatgc gtgagttgat
    16021 tatcgcggac aatgtgcacg gcgaaagcgg tctggatggc ccggcattac cggaaccgac
    16081 gttcgcaccg caaaactgta cggcggtaga gctgatggcg aaaacgctgc gcgaaagtgc
    16141 ggaacctgtc acgattgtgt ctaccggacc gcaaactaac gttgccttgc tgctcaatag
    16201 ccatccggaa ctgcatagca aaattgcccg tatcgtgatt atgggcggcg caatggggct
    16261 tggtaactgg acgcctgcgg ctgaatttaa tatttacgtt gacccggaag cggcagaaat
    16321 tgtcttccag tcagggatcc cagtggtgat ggccggtctg gatgttactc acaaagcaca
    16381 aatccacgtt gaagacaccg agcgtttccg cgcgattggc aaccctgttt caaccattgt
    16441 tgccgaactg ctggatttct tcctcgaata tcataaagac gaaaaatggg gctttgtcgg
    16501 tgcaccgctg catgacccat gcaccatcgc ctggctgttg aaacccgagt tgtttaccac
    16561 tgttgagcgc tgggttggcg tggaaacaca ggggaaatat agccagggta tgacggttgt
    16621 tgattattat tatctgactg gcaataaacc gaatgccact gtaatggtcg atgttgatcg
    16681 tcagggcttt gttgatttac tggctgatcg tctgaaattt tacgcttaac acacgtcatt
    16741 gattaagatc accacgcgtt acctgggtgg tcttaatctg tttatctgtt tatctctttt
    16801 tctttcaaga tataaatgca atggataatg cagaactcgc cattggtatc gatctcggca
    16861 ctaccaatag tttaattgcc gtctggaaag acggtgccgc gcaattaatt ccaaataagt
    16921 tcggtgaata tttaacacca tccataatta gcatggatga aaataatcat attttagtcg
    16981 gaaaaccggc tgtatcacgg cgtacttcgc atccggataa aacggcagcg ttatttaaac
    17041 gtgcaatggg cagtaatacc aactggcggt taggcagcga cacatttaac gcgccagaac
    17101 tgtcctcttt ggtattacgc tcattaaaag aagatgccga agaatttctg caacgtccga
    17161 ttaaagatgt ggtgatctcc gttccggctt atttcagcga tgaacaacgc aagcataccc
    17221 gtttagcagc ggagttagcc gggttaaatg cggtacgctt aattaatgaa cccacagcag
    17281 ctgcgatggc atatggcctg catacgcaac aaaatacccg ctcgctggtt tttgatctcg
    17341 gtggcggcac gtttgacgtc acggtgctcg aatacgccac gccggtgatt gaagttcacg
    17401 cctccgctgg cgataacttc ctcggtggcg aagattttac ccatatgttg gtcgatgacg
    17461 ttttaaagcg cgcggatgtc gccaggacca cgcttaacga gagtgaactg gcagccttgt
    17521 acgcctgtgt ggaagcggca aaatgtagca atcaatcgcc attgcacatt cgctggcagt
    17581 accaggatga aacgcgggag tgcgaatttt acgagaacga actggaagat ttgtggttgc
    17641 cgctgctcaa tcgcttgcga gtgccgattg aacaggcgtt gcgcgatgca cgtctgaagc
    17701 cgagtcaaat cgacagtctg gtgctggttg gcggcgcgtc acaaatgccg ctggtgcagc
    17761 gaatcgccgt gcgtctgttt ggcaaattac cgtatcaaag ttacgacccg agcaccattg
    17821 tcgcgctggg cgcagcaatc caggccgcct gccgcttacg cagtgaagat attgaagagg
    17881 tgatcctcac tgatatttgc ccttactcgt tgggcgttga agttaaccgc cagggcgttt
    17941 ccggcatttt ctcgccaatt attgaacgaa acaccacagt acccgtgtcg cgtgtagaaa
    18001 cttactcgac catgcacccg gaacaggatt ctattacggt taacgtctat cagggagaaa
    18061 accacaaagt taaaaataac attctggttg aatccttcga tgtgccgttg aagaaaaccg
    18121 gggcttatca gtcgattgat attcgcttta gttatgatat caacggcttg cttgaagttg
    18181 acgtgcttct ggaagatggc agcgttaagt ccagagtcat taatcatagc ccggtaacat
    18241 tgagcgcgca gcagattgaa gagagtcgga cgcggttatc cgcattgaaa atttatccgc
    18301 gcgatatgct aatcaaccgc acctttaagg ccaaactgga agagttgtgg gcgcgggcgc
    18361 tgggtgacga gcgagaagag attggccggg tgatcaccga ttttgatgcg gcgttgcagt
    18421 caaacgatat ggcccgcgtc gatgaagttc gtcgacgggc gagcgattat ttggctattg
    18481 agatccctta aacacggctg ccggatgcgg cataaacgct gcatccggca tcggcgcaaa
    18541 aaacacatcc tcagctaaac aacatcgcga tttttccgag cgcataacct atcatcagca
    18601 ccgttaagct gattttgata atactggtaa tgctctgtgg tttttcatcc tcatcatttt
    18661 tttcttcatg gttttcaggt gttggctcat cgacatcttc atcctccggc tcgagtttca
    18721 ctttcgtcaa cagcatctgg gtgataatgg cctctaatgg atgagcatct ccaccttcca
    18781 gccttgtatt aagacgggcc agcatctctt gtttcgggaa cgcttccagc acaacctgtt
    18841 ccgctacccg atacaaatcc agtaacggtg aatcttgctc tgtctcgcta ccgactctcg
    18901 atttccacca cgcccatgct ttgcgcttga agaataacga ctcaataaac gattgaccta
    18961 acggcgataa ttttccgcta tgtaaaatct ccgcccataa cagccgcgtc cgggcgctct
    19021 caccaggaac atatttcaac ggtttacctt gcgcatctac catgcttcgc gcttcgataa
    19081 ccatcactaa taacgcgaaa taatccgggc aatgacgctt gctccactgc aacaacaaat
    19141 tatcagcttg tgtagatggg taactttccc agtacgcgca gagatcggca agcaggcttt
    19201 ccccctcgcc gcagtacaaa cgttgtgcac actggtaata ccgggcatct tcgctttctg
    19261 gttcttccgc ttgccactgt ttggctacag gaatgagttc cgccatacct gtttgcaccg
    19321 agctgtacca gcgcaacagt ttgcgatgta actttgcatc atcaggaatg aaccacggtc
    19381 cttccatcgc caaaaacgca gtgacatagt caggatggta acggcagcaa cgttccagcg
    19441 cgaagtaata ttcaacagtc tggttctgta acgcgacggg atattccaga aggctaagga
    19501 aatcgaaaca atcaccagcc tgaatggcct caagaaaagc ctccctttct tcctcatcca
    19561 ttccttcgct gtcattaaac gaatgccagt tcaaacgctc tgccaacaaa gaagcacaat
    19621 ttaatgaaag atatcgcgct tctatagcga ttgcacacag cggccagcgt aattgatcga
    19681 catcctccat gttccaggta tttaattgct gaataaattt ctgccaggcg gagggctgaa
    19741 aacgatcact ttctgaatcc agtaatgtcc tgaatgcacg tagtatttca tgttcagcgg
    19801 tggcatcttt ttcttcatca tctgcttcct cgacaggatt taccgccagt cgcagcgcct
    19861 cttcataggc ctggcgtaat gctttaaacc cctgcggatc ggtttcggga tgacacaacg
    19921 gtaagcgagc aagataagcc tggcggataa tgtctatttg cgtcgtgctt tcaatttcga
    19981 ggatttgcca acatgttttc ataggtcgct cacatcctta agcgttaccg ggcattcagg
    20041 ctggacagtt aatggttgcc agggaagcgt ggcatagaaa cggtcgcttc ccgctgccag
    20101 ctcttcgaac atccgactcc cggcgctatc gtatttgtac agggttagcg cctcgcgtag
    20161 cgaagcgttg tcttcgcact gagaagattg ccagtagaga cgccctagtg aataggcaga
    20221 aaaatagtgc atccagctgt gatagtaatg gtgagctcgt gcatagacgc gcgattgcag
    20281 ccataaactc tcttcttccg tcagccagtt gttgagcaca ccaacccgtg acagaaaccc
    20341 cattcgaaca taatcccagg ctttgattcc cccttctcca cagcacatag cggtttgggc
    20401 aacgaattgg gtataaatca accctctctc cgagccacca gcaattaatg ctttccactc
    20461 ttcaggggaa gagcgttgcc atgtcagata taagccagct aaatcattgg catgaccatc
    20521 atcagtcatc tgataaatag tctgaattaa atcatcacga ttatttattc cccaggatga
    20581 tgagagatcg aattctttta tcccagtata catttttggc gagtcatgcg cgggtttgca
    20641 actctgatta agtatttcca tgggcgcaga aagcgcaaaa agaagatatt gttcttcctt
    20701 attcattttt taataatccc ttaagtgcat taactgacta cttttttatt attgatgagt
    20761 ataaacttga cgctttcaaa ataaaaagaa aatcgaagca ttcacacatg aataaaaaat
    20821 caatgtatat attcgcaatt tttatagttg cagcaattac ctgtattagc caacccaaga
    20881 aaacgacgtt gcgtgataaa gccatggtga attatgcctt tgattattta agatcaccag
    20941 gcagtctacc gttaaccacg gcagccacgg agctttccgc gattcatggt cactcaacat
    21001 cgcaatatcg ccttggcgaa ttttatcttc atggtagcga cggtaaacca ctggattata
    21061 cacaggcgag atactggtat gagcaatcag cagaacagga aaatccgcgc gcacaaagca
    21121 aactggggtg gatctacctg aaaggcctgg gagtcaaacc cgatacccgt aaagcaattc
    21181 tctggtataa ggaagcagct gaacaagggt atgctcatgc tcaatatact ttgggtttga
    21241 tctacagaaa tggcactggt attaatgtta accattatga atctcaaaaa tggttaaaac
    21301 tggccgccaa acaacattac aaaaatgcgg aaagattact tgccggactt cccgcacatt
    21361 aacgtcgact gtcacccgaa gagatggagt atttttccta tcaaaccggc aaagataaaa
    21421 atgtagaaaa taattttgat gacgcccaga cagcccagcc cgccatcgtc aacgggcatt
    21481 ggctgcggtt gcggctccgg ctcgacggtt tcctgaccgg taaaattcgc cgcctgaatt
    21541 aataatcgac gaataattaa tgcttccagt ggacaacgat cttctgcatc aaggcggctc
    21601 acaatcggca ttaatgattc ttctgtcggt tggctggcct gcactaattg tttggcaact
    21661 aaatataatg gccataaggg atgttctgta tcacgcgtga gtgagatccg cggcttacgt
    21721 cctttgtgca aaaccatctc gaccagtgaa cgccccagcg cagagagttt ttcatcgctg
    21781 agtatttcgt ataaatgaaa acgcgtacgg gcgctgtcgc cggggacata aagtagctgt
    21841 ttaccctgat cgttaaccag ttcacgagct tcaatcatca tcaccactaa cgggtaataa
    21901 tcgacccgat gctgacggca ccattgcaat attaaagcat ccgcctgggt ggagggatac
    21961 tcgcgccagt agtcacaaag ttctgccagc aagctttcgc cttctccaca atagacgcgt
    22021 tgcgcgtatt cataataacg cgcatcttca ttatcaggtt cagcttcccg ccacgaccgg
    22081 gcgtaatcca gaagttcggg gatatccagg cgacctgcgc tataccagcg gagtaaattt
    22141 ctgtgcagtt ttgcatcatc ggggatgatc actgcgcgat gttgcgctaa atacaatgtc
    22201 agccattctg gatgcgatga ccaaatccga gcgaacattt ggtaaaaatc gatggtgtca
    22261 ttttgcacgg caacgggcag atgcagaatg gtctggaagt taaaaacatt ccccttcgca
    22321 atggcatata aaaaggattc cagttcttct tcgtctattt ccccgacgtt ttcctcctgc
    22381 aaccaccgca atctttctgc taataacacc acgcactcga aggataaatg ggcagtgttc
    22441 atggcgattg tgcacagcga ccagcgtaat tcatcaatct cttccatcga gcaataattt
    22501 aattgctgaa tgaatcgctg ccaggcggag ggcagaaaac gttcgctatc agaggcaagt
    22561 aacgcgcgaa aggcgagcag aatttcatgt tctgctacct catattcttc tggttgccaa
    22621 acagatttag caggcgactg cgcaatccgg agcgcctcct catacgcctg acgaagttgt
    22681 ttaaaaccct gcggatcggt ttctggatga aaggacggta acagcgccag ataagcgcgg
    22741 cggataatat cgacgtcagt cgtttcctct atttcgagga tcttccagca atttttcata
    22801 ggtcgctcat atccttaagc gtttccgggc atgtggggta gtcagccagt ggttgccagg
    22861 acaaggcagg gtaatacgca tcatcactgg caaataattg ataaaacata tcattgccgg
    22921 aatcgtcgta ttctttacga gctaagaatt cgcgtaataa aggcagatga tcatcttcgg
    22981 gagactgcca atattgccga ccaaaggtat aaccggcaaa gtattgccgc cagttactat
    23041 aataacgtag agcccgtaga tgaatacgcg actggatcca tagactttct tcttcactca
    23101 accagttatt aagtacgccc attcgactta aaaaacccat ccggacataa tcccatgctt
    23161 taattcctcc ctcaccacag cattcggctg tgctggcgac aaattgcgca taagcctgac
    23221 cttgctcatt tagttcagca agatagtcac gccattcaca tggcgaataa cgaaaccagc
    23281 ggtgataaaa ccctgccaac cgagcggcat gaccgttatc gatcattcgg tgaattgtgc
    23341 gcagcaggtc atcacggtca tcaattcccc atgaggaaga aaggtcaaaa aagcatgtac
    23401 ctaaatacat tttgggggat gaatgcgaag gtaaacagca ttcattgcgt atttccatcg
    23461 gagtggaaag tgcaaacaat attttttgcg accccatgtc catataattt atccttaaac
    23521 attcatttaa tgaatgtaaa ctacatttcg agactcagtt atttatactt ttctgacgca
    23581 acataatacg cataaaataa ggcttacgca aaaatggaac acataaaaat ataatatgtt
    23641 ttatctacaa ccataaggaa atgataatgg tttttacgtc aagttgctgc gataatttat
    23701 caatagatga gattatcgaa cgtgctgaaa aaggcgattg cgaggctcaa tatattgttg
    23761 ggttttatta taatcgcgat agcgcaattg actctccgga cgacgaaaag gccttttact
    23821 ggctgaagct ggccgctgag caaggtcatt gtgaagcaca gtattcctta gggcaaaagt
    23881 ataccgagga taaaagccgt cataaagata atgagcaagc catcttctgg ctgaaaaaag
    23941 ctgccctaca aggccatact ttcgcttcca acgcccttgg ctggacactg gatcgtggag
    24001 aagcccccaa ttataaagaa gcggttgtct ggtatcagat agccgcggag agcggaatgt
    24061 cttatgcgca aaataatctt gggtggatgt acagaaatgg caacggagtc gcaaaagact
    24121 atgcgctggc atttttctgg tacaaacaag ctgcattaca aggccatagt gacgcgcaaa
    24181 acaatctggc cgatctttat gaagacggaa aaggcgttgc tcaaaacaag acactcgccg
    24241 cattctggta tttgaaaagc gcacagcagg gtaatcggca cgcccagttt caaattgcat
    24301 gggattataa cgctggcgaa ggggtggacc aggactataa gcaagcgatg tactggtatc
    24361 tgaaggctgc cgctcaggaa agcgtcggcg cttacgtcaa catcggttat atgtataaac
    24421 acggacaagg cgttgagaaa gattatcagg ctgcctttga atggtttatg aaagccgctg
    24481 aatgcaatga cgccactgcc tggtataacc tggccattat gtatcactac ggagaaggaa
    24541 gacctgtcga tctccgacag gctctcgacc tgtatcgtaa agttcagtca tccggaacca
    24601 gagatgtcag tcaggaaatt cgtgagactg aagatttact gtagagataa aaacaagggc
    24661 cggggaattt cattcggccc atattctctt tattgctgtg cgaaatcaga ctattacggc
    24721 tcaaacgggc gtctctggaa ctggtcatga ccacatttcg ggcatagcgt cagcacttcc
    24781 ggtgtgtaga tcgggagatg gaagtgacat ttctcgcaga ccagatttcc cagcccgacc
    24841 acttctccgc tgtgataaac cccatgatga ttgaggtcct ggaaaacttc gcgccattca
    24901 agctgcgttt tatcggtgat atctgccagc tcctgccata agctttcttt aatcacccgc
    24961 ataaagacgc tgtcagattc ttctttcagg ctctcttcat agctcatggc gaactcttcc
    25021 aggtcacgtc ttacagctcg cgtcagctca tcgacctcgg ttcgcgttaa ctcccctgtt
    25081 tttattacgc gctcgcgcgc ctgttccacc agtgcgtcga tatcacgttc gccattgcgc
    25141 aggcgttcgc tcagtgacgc gaccagttca cggtaatatt gagcaacctt gttcatcgtt
    25201 tcatctcccg ggtcgttaac tgactataat aatagacgtt ctttgttcct tgctttgcta
    25261 atacgcccac aaactctgta aattaatgta tgaataatcc gggcatcatt tcaacgtcga
    25321 gtgcgcgaaa ggctgttttg acgcggacgt ttgggctatg ctatgcggat ctgaaaaacc
    25381 acctcaacgc tacatttgta gccgtattga aaacaggacc actggctgcc atgcaagagc
    25441 aataccgccc ggaagagata gaatccaaag tacagcttca ttgggatgag aagcgcacat
    25501 ttgaagtaac cgaagacgag agcaaagaga agtattactg cctgtctatg cttccctatc
    25561 cttctggtcg actacacatg ggccacgtac gtaactacac catcggtgac gtgatcgccc
    25621 gctaccagcg tatgctgggc aaaaacgtcc tgcagccgat cggctgggac gcgtttggtc
    25681 tgcctgcgga aggcgcggcg gtgaaaaaca acaccgctcc ggcaccgtgg acgtacgaca
    25741 acatcgcgta tatgaaaaac cagctcaaaa tgctgggctt tggttatgac tggagccgcg
    25801 agctggcaac ctgtacgccg gaatactacc gttgggaaca gaaattcttc accgagctgt
    25861 ataaaaaagg cctggtatat aagaagactt ctgcggtcaa ctggtgcccg aacgaccaga
    25921 ccgtactggc gaacgaacaa gttatcgacg gctgctgctg gcgctgcgat accaaagttg
    25981 aacgtaaaga gatcccgcag tggtttatca aaatcactgc ttacgctgac gagctgctca
    26041 acgatctgga taaactggat cactggccag acaccgttaa aaccatgcag cgtaactgga
    26101 tcggtcgttc cgaaggcgtg gagatcacct tcaacgttaa cgactatgac aacatgctga
    26161 ccgtttacac tacccgcccg gacaccttta tgggttgtac ctacctggcg gtagctgcgg
    26221 gtcatccgct ggcgcagaaa gcggcggaaa ataatcctga actggcggcc tttattgacg
    26281 aatgccgtaa caccaaagtt gccgaagctg aaatggcgac gatggagaaa aaaggcgtcg
    26341 atactggctt taaagctgtt cacccattaa cgggcgaaga aattcccgtt tgggcagcaa
    26401 acttcgtatt gatggagtac ggcacgggcg cagttatggc ggttccgggt cacgaccagc
    26461 gcgactacga gtttgcctct aaatacggcc tgaacatcaa accagttatc ctggctgctg
    26521 acggctctga gccagatctt tctcagcaag ccctgactga aaaaggcgtg ctgttcaact
    26581 ctggcgaatt caacggtctt gaccatgaag cggccttcaa cgccatcgcc gataaactga
    26641 ctgcgatggg cgttggcgag cgtaaagtga actaccgcct gcgcgactgg ggtgtttctc
    26701 gtcagcgtta ctggggcgcg ccgattccga tggtgacgct ggaagacggt accgtaatgc
    26761 cgaccccgga cgaccagctg ccggtgatcc tgccggaaga tgtggtgatg gacggcatta
    26821 ccagcccgat taaagcagat ccggagtggg caaaaactac cgttaacggt atgccagcgc
    26881 tgcgtgaaac cgacactttc gacaccttta tggagtcctc ctggtactat gcgcgctaca
    26941 cttgcccaga gtacaaagaa ggtatgctgg attccgaagc ggctaactac tggctgccgg
    27001 tggatatcta cattggtggt atcgaacacg ccattatgca cctgctctac ttccgcttct
    27061 tccacaaact gatgcgtgat gcaggcatgg tgaactctga cgaaccagcg aaacagttgc
    27121 tgtgtcaggg tatggtgctg gcagatgcct tctactatgt tggcgaaaac ggcgaacgta
    27181 actgggtttc cccggttgat gctatcgttg aacgtgacga gaaaggccgt atcgtgaaag
    27241 cgaaagatgc ggcaggccat gaactggttt ataccggcat gagcaaaatg tccaagtcga
    27301 agaacaacgg tatcgacccg caggtgatgg ttgaacgtta cggcgcggac accgttcgtc
    27361 tgtttatgat gtttgcttct ccggctgata tgactctcga atggcaggaa tccggtgtgg
    27421 aaggggctaa ccgcttcctg aaacgtgtct ggaaactggt ttacgagcac acagcaaaag
    27481 gtgatgttgc ggcactgaac gttgatgcgc tgactgaaga tcagaaagcg ctgcgtcgcg
    27541 atgtgcataa aacgatcgct aaagtgaccg atgatatcgg ccgtcgtcag accttcaaca
    27601 ccgcaattgc ggcgattatg gagctgatga acaaactggc gaaagcacca accgatggcg
    27661 agcaggatcg cgctctgatg caggaagcgc tgctggccgt tgtccgtatg cttaacccgt
    27721 tcaccccgca catctgcttc acgctgtggc aggaactgaa aggcgaaggc gatatcgaca
    27781 acgcgccgtg gccggttgct gacgaaaaag cgatggtgga agactccacg ctggtcgtgg
    27841 tgcaggttaa cggtaaagtc cgtgccaaaa tcaccgttct ggtggacgca acggaagaac
    27901 aggttcgcga acgtgctggc caggaacatc tggtagcaaa atatcttgat ggcgttaccg
    27961 tgcgtaaagt gatttacgta ccaggtaaac tcctcaatct ggtcgttggc taagcgcggg
    28021 aggaagcgtg cgatatctgg caacattgtt gttatctctg gcggtgttaa tcaccgccgg
    28081 gtgtggctgg catctgcgtg ataccacgca ggttccttcc actatgaagg tcatgatcct
    28141 ggactcaggc gatccgaacg ggccattaag ccgtgcagtg cgtaaccagt tacgtctgaa
    28201 tggtgtcgag ttgcttgata aagaaaccac gcgtaaggac gttccatccc tgcgtttggg
    28261 taaagtgagc atcgcgaaag ataccgcatc ggtattccgt aacggtcaaa cagcagagta
    28321 tcagatgatc atgacggtga atgcgaccgt gttgatcccc ggccgtgata tctacccgat
    28381 tagcgccaaa gtcttccgtt cgttctttga taacccgcaa atggcgttag cgaaagataa
    28441 cgaacaagac atgatcgtaa aagagatgta cgaccgtgct gccgaacagc tgattcgtaa
    28501 gctgccaagc atccgtgctg cggatattcg ttccgacgaa gaacagacgt cgaccacaac
    28561 ggatactccg gcaacgcctg cacgcgtctc caccacgctg ggtaactgat gattcggttg
    28621 tacccggaac aactccgcgc gcagctcaat gaagggctgc gcgcggcgta tcttttactt
    28681 ggtaacgatc ctctgttatt gcaggaaagc caggacgctg ttcgtcaggt agctgcggca
    28741 caaggattcg aagaacacca cactttttcc attgatccca acactgactg gaatgcgatc
    28801 ttttcgttat gccaggctat gagtctgttt gccagtcgac aaacgctatt gctgttgtta
    28861 ccagaaaacg gaccgaatgc ggcgatcaat gaacaacttc tcacactcac cggacttctg
    28921 catgacgacc tgctgttgat cgtccgcggt aataaattaa gcaaagcgca agaaaatgcc
    28981 gcctggttta ctgctcttgc gaatcgcagc gtgcaggtga cctgtcagac accggagcag
    29041 gctcagcttc cccgctgggt tgctgcgcgc gcaaaacagc tcaacttaga actggatgac
    29101 gcggcaaatc aggtgctctg ctactgttat gaaggtaacc tgctggcgct ggctcaggca
    29161 ctggagcgtt tatcgctgct ctggccagac ggcaaattga cattaccgcg cgttgaacag
    29221 gcggtgaatg atgccgcgca tttcacccct tttcattggg ttgatgcttt gttgatggga
    29281 aaaagtaagc gcgcattgca tattcttcag caactgcgtc tggaaggcag cgaaccggtt
    29341 attttgttgc gcacattaca acgtgaactg ttgttactgg ttaacctgaa acgccagtct
    29401 gcccatacgc cactgcgtgc gttgtttgat aagcatcggg tatggcagaa ccgccggggc
    29461 atgatgggcg aggcgttaaa tcgcttaagt cagacgcagt tacgtcaggc cgtgcaactc
    29521 ctgacacgaa cggaactcac cctcaaacaa gattacggtc agtcagtgtg ggcagagctg
    29581 aaagggttat ctcttctgtt gtgccataaa cccctggcgg acgtatttat cgacggttga
    29641 tatgaaatct ttacaggctc tgtttggcgg cacctttgat ccggtgcact atggtcatct
    29701 aaaacccgtg gaaacgctgg cgaatttgat tggtctgacg cgggtcacaa tcatccctaa
    29761 taatgttcct ccgcatcgtc cccagccgga agcgaacagc gtgcagcgta aacacatgct
    29821 tgaactggcg attgccgaca agccattatt tactcttgat gaacgcgagc taaagcgcaa
    29881 tgccccctct tacactgcgc aaacactgaa agagtggcgg caggaacaag gaccggacgt
    29941 gccgctggcg tttattattg gtcaggattc actgctgacc tttccgacct ggtacgaata
    30001 cgaaacgata ctcgacaatg cacatttgat cgtctgtcgg cgtccaggtt acccacttga
    30061 aatggcgcaa ccgcaatacc agcaatggct ggaagatcat ttgacacata acccggaaga
    30121 tcttcacctt cagcctgccg gtaaaattta tctggctgaa acgccgtggt ttaacatctc
    30181 ggcgaccatc atccgcgaac gtttgcaaaa cggtgaatcg tgtgaggatt tattgccgga
    30241 accggtactg acttacatta accaacaagg cttgtatcgc tgatacctgt cgttacggag
    30301 ggcatgatgc gactgtggtt aattcgtcat ggtgaaacgc aagcgaatat cgatggtctt
    30361 tacagcggtc atgcgcccac ccccctgacc gcgcgcggta ttgagcaagc gcaaaatctg
    30421 catacgctgc tacatggtgt ttcctttgat ctggttttat gcagtgaact ggaacgggca
    30481 cagcataccg cacgactggt tctcagtgac cgccagctcc ccgtgcaaat catccctgaa
    30541 ctcaacgaaa tgttttttgg cgactgggag atgcgacatc atcgcgacct catgcaagaa
    30601 gatgccgaaa actatagcgc gtggtgcaat gactggcagc atgcaatccc cacgaacggt
    30661 gaaggatttc aggcattttc gcaacgtgtg gaacgcttta tcgcaaggct tagtgaattt
    30721 cagcactatc agaatatttt agtcgtcagc catcagggtg tactgagtct gttaatcgcc
    30781 cgtttaattg gcatgcctgc cgaggctatg tggcattttc gcgttgacta ggggtgttgg
    30841 agcgccattg atatcaacca aaaattcgcg acgctacgcg tcctcaatag ccgtgccatc
    30901 ggggtcgaga atgcatgact tttctgtttt tttacgggta agccgcaacg accattgaca
    30961 gacccgggca ggctgatatt ctccgcagcc agactttttt cgccagacac gactttgtag
    31021 aaattgtttt acaaaaatgg cgatgcaatc tgcggcgcgg ggtgggatga tagcccactt
    31081 tcgaaagccg attcggcgac aattgtcccg aaatcgcctc tggttcaggt atactgacag
    31141 accattttta tctatttgat tcacccaggg ggaaaacttg cagggtaaag cactccagga
    31201 ttttgttatc gacaaaattg atgacctcaa aggtcaggac atcatcgcct tagacgttca
    31261 aggcaaatcc agcatcaccg actgcatgat catctgtacg ggtacgtcca gccgtcatgt
    31321 tatgtccatt gctgaccacg ttgtgcagga gtctcgcgca gcgggcctgt taccgctcgg
    31381 cgtagaaggt gaaaacagcg ccgactggat tgtcgtggat ttgggcgatg tgattgtcca
    31441 tgtcatgcag gaagagagcc gtcgcctgta tgaactggaa aaactctgga gttaatgcgt
    31501 gaagctgcaa cttgtcgccg tgggaacgaa aatgccggac tgggtacaaa ccggttttac
    31561 cgagtacctg cgtcgttttc cgaaagatat gcccttcgag ctgattgaaa ttccggccgg
    31621 aaaacgcggc aagaatgcgg acatcaagcg catactcgac aaagagggtg agcagatgtt
    31681 ggcggccgca ggcaaaaacc gcattgtcac cctcgatatt ccaggcaagc cctgggatac
    31741 gccgcagtta gccgctgagc tggaacgctg gaagctggat ggtcgcgacg tcagtctact
    31801 gattggcggg cctgaagggt tgtcgcctgc ctgtaaagcg gcggcggagc agagctggtc
    31861 gctgtcggcg cttaccctcc cccatccgct ggttcgcgtg ctggtcgcag agagtctgta
    31921 ccgggcgtgg agcatcacca ccaaccatcc ttatcaccgt gagtgataag ggagctttga
    31981 gtagaaaacg cagcggatga aactacagaa ctcttttcgc gactatacgg cagagtccgc
    32041 gctgtttgtg cgccgggcgc tggtcgcctt tttggggatt ttgctgctga ccggcgtgct
    32101 tatcgccaac ctgtataatc tgcaaattgt tcgctttacc gactaccaga ctcgctctaa
    32161 tgaaaaccgc attaagctgg tgcctatcgc gcccagccgc ggcattatct acgaccgtaa
    32221 tggtatccct ctggccctca accgcactat ctaccagata gaaatgatgc cagagaaagt
    32281 cgataacgtg cagcaaacgc tggacgcttt gcgcagcgtg gttgatctga ccgatgacga
    32341 tattgctgca ttccgaaaag agcgcgcacg ttcacaccgt ttcacctcta ttccggtgaa
    32401 aactaacctg accgaagtac aagtagctcg ctttgccgtc aatcagtacc gttttccggg
    32461 tgtcgaagtt aaaggctata aacgtcgtta ctatccttac ggttcggcgt tgacccacgt
    32521 catcggctat gtgtcgaaaa tcaacgataa agacgtcgaa cgcctgaata atgacggcaa
    32581 actggccaac tatgcggcaa cgcatgatat cggtaagctg ggcattgagc gttactatga
    32641 agacgtgctg cacggtcaga ccggttatga agaggttgaa gttaacaacc gtgggcgtgt
    32701 tatccgccag ttaaaagaag taccaccgca agccggacac gatatttacc tgacgctgga
    32761 tctcaaactc cagcaatata ttgaaacgct gctggcgggt agccgcgcag ctgtggttgt
    32821 caccgatccg cgtacaggtg gggtgctggc gctggtttcc acgcctagtt atgacccaaa
    32881 cctgtttgtt gacggtatct ccagcaaaga ttattccgcc ttgttgaacg ccccgaatac
    32941 accgctggtg aaccgcgcca cacagggggt ttatcctccc gcgtctacag ttaaacccta
    33001 tgtggcggtt tcggcattga gcgccggggt gatcacgcgc aatacgacgc tgtttgaccc
    33061 aggctggtgg caactgccag gttcggaaaa acgttatcgt gactggaaaa aatggggcca
    33121 cgggcgtctg aatgtcacaa gatcgctgga agaatctgcg gataccttct tctatcaggt
    33181 ggcctacgat atggggatcg atcgcctctc cgaatggatg ggtaaattcg gttatggtca
    33241 ttacaccggt atcgacctgg cggaagaacg ttccggcaac atgcctaccc gcgaatggaa
    33301 acagaaacgc tttaaaaaac cgtggtatca gggtgacacc attccggttg gtatcggtca
    33361 gggttactgg acagcgaccc caatccagat gagtaaggca ctgatgatcc tgattaatga
    33421 cggtatcgtg aaggttcctc atttgctgat gagcaccgcc gaagacggca aacaggtgcc
    33481 atgggtacag ccgcatgaac cgcccgtcgg cgatattcat tccggttact gggagctggc
    33541 gaaagacggt atgtacggtg ttgctaaccg ccctaacggt acggcgcata aatactttgc
    33601 cagcgcaccg tacaaaattg cggcgaaatc cggtaccgct caggtcttcg gtctgaaagc
    33661 gaacgaaacc tataatgcgc acaaaattgc cgagcgttta cgtgaccaca aactgatgac
    33721 cgcctttgcg ccatacaaca atccgcaagt ggctgtcgcc atgattctgg agaacggtgg
    33781 tgcgggtccg gcggttggta cactgatgcg ccagatcctc gaccacatta tgctgggtga
    33841 taacaacacc gatctgcctg cggaaaatcc agcggttgcc gcagcggagg accattaatc
    33901 atgacggata atccgaataa aaaaacattc tgggataaag tccatctcga tcccacaatg
    33961 ctgctgatct tactggcatt gctggtttac agcgccctgg ttatctggag cgccagcggt
    34021 caggatattg gcatgatgga gcgtaaaatc ggccaaatcg cgatgggtct agtcatcatg
    34081 gtggtgatgg cgcaaattcc tccacgcgtt tatgaaggct gggcccccta tctctatatc
    34141 atctgtatta ttttgctggt ggcggtagat gctttcggtg ccatctctaa aggtgctcaa
    34201 cgctggctgg acctcggtat tgttcgtttt cagccgtcgg aaattgccaa aatagccgta
    34261 ccactgatgg ttgcgcgctt tatcaaccgc gacgtttgcc cgccatcgtt gaagaacact
    34321 ggcatcgcgc tggtgctgat atttatgccc acgctgctgg tggctgcaca gcctgacctg
    34381 ggaacatcaa tcctcgttgc gctttccggt ctgtttgtac tgttcctctc tggccttagc
    34441 tggcgtctga ttggcgtcgc agtagtgctg gtggcggcgt tcattccgat tctgtggttc
    34501 ttcctgatgc atgattacca gcgccagcgc gtaatgatgc tcctagaccc ggaatcagac
    34561 ccactcggcg cgggctatca cattattcag tctaaaattg ctattggctc cggcggatta
    34621 cgcggcaaag gctggctgca cggcactcag tcacagcttg aatttctccc cgaacgccat
    34681 accgacttta tcttcgcggt actggcggaa gagctgggat tagtgggcat tctgattctg
    34741 ctcgctctct acattctgct gatcatgcgc gggctgtgga tagccgccag agcgcaaacc
    34801 acctttggtc gcgtcatggc tggtggctta atgctgatat tattcgttta tgtcttcgta
    34861 aatattggta tggtaagcgg tattctgccg gttgtagggg ttccgctccc actggtcagt
    34921 tatggaggat cggcgctaat tgtgctgatg gctgggttcg ggattgtaat gtcaatccac
    34981 acccacagga aaatgttgtc gaaaagcgtg taagaggtgc gcaatgcgta agcagtggct
    35041 cgggatctgc atcgcggcag gaatgctcgc ggcatgtaca agcgatgatg gtcagcaaca
    35101 gacggtaagt gtaccgcagc ctgcggtatg taacggcccc atagttgaaa ttagcggggc
    35161 ggacccgcgt ttcgaaccac tgaacgcgac ggcaaatcag gattaccagc gcgacggtaa
    35221 aagctacaaa atcgtgcagg atccgtctcg atttagccag gcgggactgg cggcaatcta
    35281 tgatgccgaa cccggcagta acctgacggc ctctggcgaa gctttcgatc cgacacagct
    35341 gacggcggcc catccaacgc ttccgatccc cagctacgcc agaatcacta acctggctaa
    35401 cgggcgaatg atcgtggtgc gcattaatga tcgcggccct tacggcaacg accgcgttat
    35461 ttcactttct cgcgcggcag ctgaccgtct taacacgtca aacaacacca aagttcgtat
    35521 cgatccgatt attgtcgccc aggatggttc gctttctggt cctggtatgg cttgtaccac
    35581 agtcgccaaa cagacttacg ccctgccagc acctcccgat ttaagcggtg gcgcgggaac
    35641 aagttcagtg tctggcccgc agggtgacat tcttccggtc agtaattcga cgctaaaaag
    35701 cgaagatccg accggcgcgc cggtaaccag tagcggtttc ctcggcgctc caacgacctt
    35761 agcgcctggt gtactggaag gcagcgaacc gacgcctgcg ccacagcccg ttgttacagc
    35821 tccgtcgaca acgcctgcaa cctcgcctgc aatggtgaca ccgcaagccg cctcgcaaag
    35881 cgccagcggc aactttatgg tgcaagtcgg ggccgtaagc gatcaggctc gtgcgcaaca
    35941 gtaccaacag caactgggac agaagttcgg cgtccccggt cgcgtaactc aaaatggcgc
    36001 ggtctggcgg atccagcttg gcccattcgc cagcaaagcc gaagccagta ccttgcagca
    36061 acgtttgcaa accgaagccc aattacagtc atttattact accgcgcagt agcgtaaagc
    36121 aggcatctga tgtgtcaatt tctgtaaatg acgttaacaa agtcacgcgg aaagtcagat
    36181 gcctgccggt agtgcatttg ctatagtagg gcactttttt aattccatca cggatgtcgt
    36241 agttcagacc atgaatacca ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac
    36301 ggctctttgc acagccttta tctctgctgc acatgccgat gacctgaata tcaaaactat
    36361 gatcccgggt gtaccgcaga tcgatgcgga gtcctacatc ctgattgact ataactccgg
    36421 caaagtgctc gccgaacaga acgcagatgt ccgccgcgat cctgccagcc tgaccaaaat
    36481 gatgaccagt tacgttatcg gccaggcaat gaaagccggt aaatttaaag aaactgattt
    36541 agtcactatc ggcaacgacg catgggccac cggtaacccg gtgtttaaag gttcttcgct
    36601 gatgttcctc aaaccgggca tgcaggttcc ggtttctcag ctgatccgcg gtattaacct
    36661 gcaatcgggt aacgatgctt gtgtcgccat ggccgatttt gccgctggta gccaggacgc
    36721 ttttgttggc ttgatgaaca gctacgttaa cgcactgggc ctgaaaaata cccacttcca
    36781 gacggtacat ggtctggatg ctgatggtca gtacagctcc gcgcgagata tggcgctgat
    36841 cggccaggca ttgatccgtg acgtaccgaa tgaatactcg atctataaag aaaaagaatt
    36901 tacgtttaac ggtattcgcc agctgaaccg taacggcctg ttatgggata acagcctgaa
    36961 tgtcgacggc atcaaaaccg gacacactga caaagcaggt tacaaccttg ttgcttctgc
    37021 gactgaaggc cagatgcgct tgatttctgc ggtgatgggc ggacgtactt ttaaaggccg
    37081 tgaagccgaa agtaaaaaac tgctaacctg gggcttccgt ttcttcgaaa ccgttaaccc
    37141 actgaaagta ggtaaagagt tcgcctctga accggtttgg tttggtgatt ctgatcgcgc
    37201 ttcgttaggg gttgataaag acgtgtacct gaccattcca cgtggtcgca tgaaagatct
    37261 gaaagccagc tatgtgctga acagcagtga attgcatgcg ccgctgcaaa agaatcaggt
    37321 cgtcggaact atcaacttcc agcttgatgg caaaacgatc gagcaacgcc cgctggttgt
    37381 gttgcaagaa atcccggaag gtaacttctt cggcaaaatc attgattaca ttaaattaat
    37441 gttccatcac tggtttggtt aaaaattgaa cacttgaaag tgtaatttcc gtccccatat
    37501 actaagcatc agtaaaaaac tcccgccttc tggcgggagt tgctatttaa ttacgttacg
    37561 ccggagctga catgaaaacc aaacttaacg aactgcttga attccctact ccttttactt
    37621 acaaagttat ggggcaggcg ttacctgagc tggttgatca ggtggttgaa gtggtacagc
    37681 gccatgcgcc aggtgactac accccaacgg taaaaccaag cagcaaaggc aactaccact
    37741 cggtatctat cactatcaac gccactcata tcgagcaggt tgaaacactg tatgaagaac
    37801 taggcaaaat cgatattgtc cgcatggttc tgtaactcgc ttctccgtta cccggcccct
    37861 ggtcgggtaa ctcccctctc ctcgctgtga tatacttttc ccccactttt actcattctc
    37921 cacggagatg cagttttgta tcaggataaa attcttgtcc gccagctcgg tcttcagcct
    37981 tacgagccaa tctcccaggc tatgcatgaa ttcaccgata cccgcgatga tagtaccctt
    38041 gatgaaatct ggctggtcga gcactatccg gtattcaccc aaggtcaggc aggaaaagcg
    38101 gagcacattt taatgccggg tgatattccg gtgatccaga gcgatcgcgg tgggcaggtg
    38161 acttatcacg ggccggggca acaggtgatg tatgtgttgc ttaacctgaa acgccgtaaa
    38221 ctcggtgtgc gtgaactggt gaccttgctt gagcaaacag tggtgaatac cctggctgaa
    38281 ctgggtatag aagcgcatcc tcgggctgac gcgcctggtg tctatgtcgg ggaaaagaaa
    38341 atttgctcac tgggtttacg tattcgacgc ggttgttcat tccacggtct ggcattaaac
    38401 gtcaatatgg atctttcacc atttttacgt attaatcctt gtgggtatgc cggaatggaa
    38461 atggctaaaa tatcacaatg gaaacccgaa gcgacgacta ataatattgc accacgttta
    38521 ctggaaaata ttttagcgct actaaacaat cctgacttcg aatatattac cgcttaattg
    38581 catacctcaa tggcccaatt tacactgggt cattactcat aatacctgct aatgctttac
    38641 atctccttcg cgtttgtata ctttctttat cacttgcaga aatgcggtgc tacgccttgc
    38701 tccgtaactc accccattga ttaacctatt gaaatagttg attatggtga gcaaaagcgt
    38761 tccgttccaa ttagacataa gattattgta atttttttaa atattatttt tccatgaata
    38821 aaattggagt aagtgcgtgg atagtaataa tcaaattgaa ccctgtttaa gcagaaagtc
    38881 gtcagaaggt aaaccacaaa tatttacaac cttacgaaat attgatctta accttctgac
    38941 tatttttgaa gctgtatatg tacataaagg gatcgttaat gcagcgaaag tgcttaatct
    39001 gaccccctcg gcaatcagtc agtctattca gaaactgcgc gttatattcc ctgacccatt
    39061 gtttattcgc aaaggccagg gtgtcactcc taccgcattt gcgatgcatc tacatgagta
    39121 tatcagtcag ggccttgagt ccattcttgg cgcgctggat atcgaaggaa gctatgataa
    39181 gcaacgaacg ataactattg cgaccactcc ctcggtcgga gccctggtcc ttcctgtcat
    39241 ctatcgggcg attaaaactc actatccgca gcttttactg cgcaacccgc ccatcagcga
    39301 cgcggaaaac caactcagtc agtttcaaac cgatctcatc atcgataaca tgttttgcac
    39361 caatcgtacg gtgcaacatc atgttctgtt caccgacaat atggtgttaa tttgccgcga
    39421 gggaaatcca ctactctctt tagaagatga cagagagact atcgacaacg ctgcgcatgt
    39481 actcctgtta ccggaagggc aaaatttcag cggtctgcgg cagagagttc aagagatgtt
    39541 tccggaccgg caaatcaatt tcaccagcta caacattttg acaatcgctg cactggttgc
    39601 caacagtgac atgttagcga ttattcccag ccgtttttat aacctgttta gtcgttgctg
    39661 gccgctggaa aaactgcctt ttccgtcctt aaatgaggag caaatagatt tctctatcca
    39721 ctacaacaaa ttcagcctgc gtgatcccat cctgcacggg gtaatagatg ttatccgtaa
    39781 tgcattttga aaaagtattg ttacagccag atcacagcgc agaacgataa aatggcacaa
    39841 tccaacaaca attttacatt ttcgcgaccg ctttggctgc tttcaggtcc gtttcaatga
    39901 tatactgcca gtcgttaatt caaaaatagt tgataattac aacaatctat tgaattgaaa
    39961 cgctttcctt cgtaattcgc aactggaaca cgcacgctat gagtaaaccc attgtgatgg
    40021 aacgcggtgt taaataccgc gatgccgata agatggccct tatcccggtt aaaaacgtgg
    40081 caacagagcg cgaagccctg ctgcgcaagc cggaatggat gaaaatcaag cttccagcgg
    40141 actctacacg tatccagggc atcaaagccg caatgcgcaa aaatggcctg cattctgtct
    40201 gcgaggaagc ctcctgccct aacctggcgg aatgcttcaa ccacggcaca gcaacgttta
    40261 tgatcctcgg cgctatttgt acccgccgtt gtccgttctg tgatgttgcc cacggtcgcc
    40321 cggtagctcc tgatgccaat gaaccagtga aactggcgca gaccattgcc gatatggcgc
    40381 tgcgttatgt ggttatcacc tccgttgacc gtgatgacct gcgcgatggc ggtgcccagc
    40441 actttgcgga ttgcattact gccattcggg aaaaaagccc gcaaatcaaa attgaaactc
    40501 tggtgccgga tttccgtggt cgtatggatc gtgctctgga tattctgact gcaacgccac
    40561 cagatgtgtt caaccataac ctggaaaacg taccgcgtat ttaccgtcag gtacggcctg
    40621 gtgcagatta caactggtcg ctgaagctgc tggaacgctt taaagaagcg catccggaaa
    40681 tcccgaccaa gtctggtctg atggtgggac tgggtgaaac caatgaagaa attattgagg
    40741 taatgcgcga cctgcgccgt catggtgtga cgatgttaac gctggggcaa tatttgcagc
    40801 caagccgcca tcacctgccg gttcaacgtt acgttagccc ggatgagttc gacgaaatga
    40861 aagccgaagc gctggcgatg ggctttaccc atgctgcatg cggtccgttt gtccgctctt
    40921 cttaccacgc cgatttgcag gcgaaaggga tggaagttaa gtaatcctgt aataattact
    40981 tacatctgtc tgataaaaaa cccgcttttt g
//
LOCUS       DAZPLI010000021        40107 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZPLI010000021 DAZPLI010000000
VERSION     DAZPLI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.021"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="ACHUU3_001055"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="ACHUU3_001055"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR2081193.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHUU3_001056"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHUU3_001056"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR2081194.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHUU3_001057"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHUU3_001057"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR2081195.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHUU3_001058"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHUU3_001058"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR2081196.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHUU3_001059"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHUU3_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR2081197.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHUU3_001060"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHUU3_001060"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR2081198.1"
                     /translation="MKSCRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="ACHUU3_001061"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="ACHUU3_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR2081199.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHUU3_001062"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHUU3_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR2081200.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="ACHUU3_001063"
     CDS             6537..6773
                     /locus_tag="ACHUU3_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR2081201.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="ACHUU3_001064"
     CDS             6776..7633
                     /locus_tag="ACHUU3_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR2081202.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="ACHUU3_001065"
     CDS             7654..9645
                     /locus_tag="ACHUU3_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR2081203.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHUU3_001066"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHUU3_001066"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR2081204.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="ACHUU3_001067"
     CDS             complement(10248..11144)
                     /locus_tag="ACHUU3_001067"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR2081205.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="ACHUU3_001068"
     CDS             complement(11193..11432)
                     /locus_tag="ACHUU3_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR2081206.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="ACHUU3_001069"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="ACHUU3_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR2081207.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="ACHUU3_001070"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="ACHUU3_001070"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR2081208.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMEE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="ACHUU3_001071"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="ACHUU3_001071"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR2081209.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="ACHUU3_001072"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="ACHUU3_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR2081210.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="ACHUU3_001073"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="ACHUU3_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHUU3_001074"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHUU3_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR2081211.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="ACHUU3_001075"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="ACHUU3_001075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR2081212.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="ACHUU3_001076"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="ACHUU3_001076"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR2081213.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22739)
                     /locus_tag="ACHUU3_001077"
     CDS             complement(21570..22739)
                     /locus_tag="ACHUU3_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2081214.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22905..22994)
                     /gene="cydH"
                     /locus_tag="ACHUU3_001078"
                     /gene_synonym="ynhF"
     CDS             complement(22905..22994)
                     /gene="cydH"
                     /locus_tag="ACHUU3_001078"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR2081215.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23293..24318
                     /gene="purR"
                     /locus_tag="ACHUU3_001079"
     CDS             23293..24318
                     /gene="purR"
                     /locus_tag="ACHUU3_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR2081216.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24315..25247)
                     /gene="punR"
                     /locus_tag="ACHUU3_001080"
     CDS             complement(24315..25247)
                     /gene="punR"
                     /locus_tag="ACHUU3_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR2081217.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25360..26571
                     /gene="punC"
                     /locus_tag="ACHUU3_001081"
     CDS             25360..26571
                     /gene="punC"
                     /locus_tag="ACHUU3_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR2081218.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26862..28010
                     /gene="cfa"
                     /locus_tag="ACHUU3_001082"
     CDS             26862..28010
                     /gene="cfa"
                     /locus_tag="ACHUU3_001082"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR2081219.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="ACHUU3_001083"
     CDS             complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="ACHUU3_001083"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR2081220.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28906..30279
                     /gene="mdtK"
                     /locus_tag="ACHUU3_001084"
     CDS             28906..30279
                     /gene="mdtK"
                     /locus_tag="ACHUU3_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR2081221.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNKKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30320..31576)
                     /locus_tag="ACHUU3_001085"
     CDS             complement(30320..31576)
                     /locus_tag="ACHUU3_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR2081222.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31884..31960
                     /locus_tag="ACHUU3_001086"
     tRNA            31884..31960
                     /locus_tag="ACHUU3_001086"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31918..31920,aa:Val,seq:gac)
     gene            31965..32041
                     /locus_tag="ACHUU3_001087"
     tRNA            31965..32041
                     /locus_tag="ACHUU3_001087"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31999..32001,aa:Val,seq:gac)
     gene            32149..32454
                     /locus_tag="ACHUU3_001088"
     CDS             32149..32454
                     /locus_tag="ACHUU3_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR2081223.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32580..34184
                     /locus_tag="ACHUU3_001089"
     CDS             32580..34184
                     /locus_tag="ACHUU3_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFR2081224.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34196..35008)
                     /gene="ydhT"
                     /locus_tag="ACHUU3_001090"
     CDS             complement(34196..35008)
                     /gene="ydhT"
                     /locus_tag="ACHUU3_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR2081225.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEGGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35012..35797)
                     /gene="phsC"
                     /locus_tag="ACHUU3_001091"
     CDS             complement(35012..35797)
                     /gene="phsC"
                     /locus_tag="ACHUU3_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR2081226.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35794..36462)
                     /locus_tag="ACHUU3_001092"
     CDS             complement(35794..36462)
                     /locus_tag="ACHUU3_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR2081227.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36526..37164)
                     /locus_tag="ACHUU3_001093"
     CDS             complement(36526..37164)
                     /locus_tag="ACHUU3_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR2081228.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37177..39279)
                     /locus_tag="ACHUU3_001094"
     CDS             complement(37177..39279)
                     /locus_tag="ACHUU3_001094"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFR2081229.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(39300..39926)
                     /locus_tag="ACHUU3_001095"
     CDS             complement(39300..39926)
                     /locus_tag="ACHUU3_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR2081230.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         9623 a        10542 c         9887 g        10055 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcga
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgtg caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcacgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggagg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc catttttagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaggg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtggca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gacgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcgaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg
    22741 ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca
    22801 gcaatcacaa aaaaatgaaa ataacaaaaa gagaaagcac ttttgccatt ttgctaacaa
    22861 acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca
    22921 gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta
    22981 aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca
    23041 tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac
    23101 ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat
    23161 tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg
    23221 taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg
    23281 gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca
    23341 actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg
    23401 tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt
    23461 aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag
    23521 atcattgaag cagttgaaaa aaattgcttc cagaaaggtt ataccctgat tctgggcaat
    23581 gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc
    23641 gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag
    23701 tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat
    23761 gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc
    23821 ggtcaccgcg aaatcggcgt tatccccggc ccgctggaac gtaacactgg cgcaggccgc
    23881 cttgtcggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga aagctggatt
    23941 gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag
    24001 ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt
    24061 gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctaattgg ttatgataac
    24121 gtgcgcaacg cccgctattt tacgccggcg ctgaccacaa tccatcagcc aaaagattcg
    24181 ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg
    24241 cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc
    24301 cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc
    24361 ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc aggcgacata
    24421 tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc cagttcaagt
    24481 gctacccact tcccctcatt aagccacggt ttggcgaaat gtgttggcac catccctatg
    24541 cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt
    24601 tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc
    24661 aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg
    24721 tgatggctgg caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga
    24781 ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc
    24841 gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg
    24901 cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg
    24961 atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc
    25021 atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc
    25081 acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg
    25141 gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca
    25201 ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa
    25261 aatttttgaa accaatcatc aaatattacc gtttcacaac actaatttca ctccctacac
    25321 tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt
    25381 ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct
    25441 gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt
    25501 agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct ctccgaccgt
    25561 tatggtcgta aaccggtatt attaatcggt atgacaattt ttgcgttagg tagtctgggg
    25621 atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt
    25681 gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa
    25741 gttaaccgaa tttttgcgac catcataccg ctggtgggtc tatctccggc cctggctcct
    25801 ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt
    25861 gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt
    25921 aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc
    25981 aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg
    26041 ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa
    26101 actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc
    26161 aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct
    26221 gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg
    26281 attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacac
    26341 gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca
    26401 agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg
    26461 atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagct
    26521 ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg
    26581 atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat
    26641 actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt
    26701 cccgactcac tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc
    26761 ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg
    26821 ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag
    26881 aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg
    26941 ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt
    27001 ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt
    27061 gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc
    27121 aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc
    27181 agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt
    27241 tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata
    27301 atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac
    27361 ctgggatgcg cgtactggat attggctgcg gctggggcgg actggcacac tacatggcat
    27421 ctaattatga cgtaagcgtg gtgggcgtca ccatttctgc cgaacagcaa aaaatggctc
    27481 aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg
    27541 accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg
    27601 atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggcatattc ctgctccata
    27661 ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc
    27721 cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc cactttgtga
    27781 tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac
    27841 gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt
    27901 ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc
    27961 aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct
    28021 atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca
    28081 ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca
    28141 atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt
    28201 tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata
    28261 ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact atcctggact
    28321 ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc
    28381 ataatatgac ctgacattaa gtgtccgcca atttcatcgc tgaattttgc cgcacgctca
    28441 acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc
    28501 atcaggtcaa aactgacatg gttgccgtta atttccgtca cggtcaggca gcaaccgtta
    28561 tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca
    28621 tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc
    28681 cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca
    28741 ggtggaaaac gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta
    28801 ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag
    28861 gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc
    28921 agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa
    28981 actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg
    29041 gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg
    29101 ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg
    29161 catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg
    29221 ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac
    29281 aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc caggctatct tttcttccag
    29341 gttgcccgta accagtgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt
    29401 atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg
    29461 cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggttatgttc
    29521 cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatatccg taacaaaaaa
    29581 ggcaccgcaa aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt
    29641 gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg
    29701 ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc actaatgttc
    29761 gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag
    29821 ggctcaacgc tggatgcgca aaccgctgct cggaccgggc ttatggtggg tgtctgtatg
    29881 gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac
    29941 gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag
    30001 atttctgact caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt
    30061 tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt
    30121 ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt
    30181 attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt
    30241 atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc
    30301 acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg
    30361 gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta
    30421 tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg
    30481 tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg
    30541 ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag
    30601 tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag
    30661 taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta
    30721 atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt
    30781 ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg
    30841 taccggcatg cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg
    30901 aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag
    30961 tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg
    31021 ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac
    31081 ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa
    31141 ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac
    31201 gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt
    31261 caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct
    31321 cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac
    31381 cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga
    31441 ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg
    31501 tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt
    31561 tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa
    31621 gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa acaattctgt
    31681 ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa
    31741 atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga
    31801 aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatatttg
    31861 tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac
    31921 atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt
    31981 tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc
    32041 agattttctt aatctggtct tttccttttt ccctctgttt attctctgta tccaatacgt
    32101 taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg
    32161 ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa
    32221 ccacttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt
    32281 gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc
    32341 tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa
    32401 gttttcgatg tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat
    32461 ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa
    32521 tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa
    32581 tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc
    32641 tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg
    32701 ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg
    32761 aaataccgcc gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc
    32821 tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc
    32881 tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa
    32941 aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg
    33001 gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg
    33061 ccacgggtca cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt
    33121 ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct
    33181 tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg
    33241 ataaacaaca cgtcgttttt caccgcgata acgcaagtga aaagctaaat atcacgttga
    33301 tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct
    33361 tacacatcgt caccgatcag gcattaaatg ctgagattca aaaaggcgaa tatggccttt
    33421 tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc
    33481 aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc
    33541 gcaaacaacg cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata
    33601 aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac
    33661 aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa ggccttgccc
    33721 gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcgg ctgctggcct
    33781 tacgtgaagc ggggatcatt catattctcg ccctcggtga agactacaaa atggaaatta
    33841 atgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac gtttttattg
    33901 atgcccgcgg acagcgtccg cttaaagtga aagatattcc tttccctggg ctacgcgagc
    33961 aattacagaa aacaggggat gaaatccctg atgttggcga agattatacg ttacagcaac
    34021 ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc
    34081 ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg
    34141 taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc
    34201 actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt
    34261 caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc
    34321 ttccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt
    34381 caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa
    34441 agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac
    34501 aaacgcgtac tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc
    34561 gcgaagaatt accggttaca gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt
    34621 taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc
    34681 cagcggttac gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc
    34741 gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg
    34801 aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca
    34861 gactctccgc ccagtccgta tatcctccct caatcagtgc atgatgaata tcggcataac
    34921 taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt
    34981 ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc
    35041 ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatgt ccgaagataa
    35101 aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag
    35161 gaaacacatc tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg
    35221 gcagcaatcc acacatgaca ccaacatagg cgacctgctg taagggatta aatttagact
    35281 gtgttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgtg
    35341 tttgttttgc cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat
    35401 ccccaacggc attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa
    35461 cttcatgcac cgcaaccaga cttttaaccg cagttgcgcc caccatcgca aagtgattta
    35521 tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga
    35581 ccgctttgct gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac
    35641 gagcacgaag caaggcgtgc agccccaaca ccaaccacat cccaaccagc aatactccgg
    35701 caatgatcaa ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact
    35761 ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt taatcaaatg
    35821 ttgaccgaac cggcgataca gatgcggttt cccggcacca agtagctgat attgatagta
    35881 tttattttcc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc
    35941 gtgttccgga caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc
    36001 gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa cctggtacgg
    36061 acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc
    36121 ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag catcttcgca
    36181 gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg
    36241 aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc
    36301 gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac
    36361 ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac
    36421 tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc
    36481 agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa
    36541 ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc
    36601 gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg
    36661 tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc
    36721 ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa aacgaacggc
    36781 gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt
    36841 ttcactcatg gcctgcgttg agtaatagta atcatcctga cttcccgtca tacaagcgat
    36901 atccgcgtag tctccgttct gcttaatgct ttccagcaaa actgacagtt cctcttctgc
    36961 aaccgaatag ggtggcgaga gaaacacggt gcgcgccacc agctgcccag ccgcagaatg
    37021 ctggcgaatg aactcagcca ggatttcagc aggttccggt tcggttaccg tatcaacggg
    37081 gtgacgcatc ccctgcaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa
    37141 cggcaattca tcctgatggt tcatcttacc cattccttac gcaggcagta gattgtgtgc
    37201 tgccagatcg gcggcaatat cttccagccc cagacgctgc aatgtttcgc gggttggaca
    37261 accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc
    37321 gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa
    37381 aacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca tcagttttac
    37441 cgtgtaggca cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt
    37501 catctcttcg ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta
    37561 attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca gggtgacggc
    37621 gttatgcaaa cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat
    37681 tggcgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagtt ctttcgccac
    37741 ttcacgttgc agtttcaatg gcaagccgga accaataaag ttgatatggg tatgcgtcat
    37801 gcagtcacgg ttgaacatac agttaacaat ggaaccgacc tgcgctgacg cttcattggc
    37861 atggtgaacc ggatagccaa acggcgacca gagtttattt ttcgcgtagc cccagtactc
    37921 ttcacccaaa ttccagcgtt ctgcgatggc atatgaacca tcagccaggt gactcagctc
    37981 acccacacga tgcgccagac ggtagtaaaa atctttaatg aagttaacgt cacccgcttc
    38041 cagttgatcc cagcgaattt ccgcatactc ttcagcaggc agaacacgct tgaacacacc
    38101 tttgctgtaa caataggtaa agtcgcgatg caactgcccg tagttacacc ataggccgta
    38161 gtcatcgaac agattcagac ccaccaggtt accaatcaca cgaccatcgt ctttatcttc
    38221 aaaatctttc ggaccgttcg ggaagatggt ggtatggaca aagttggcta cacaagtgtt
    38281 accacctgtg ctgggcacgc caaactcttt cacccgagga atattcattt gggtcataca
    38341 acggatcggg caagagtggc agccactcat ttttactgtg tatttctctg ccgccggacc
    38401 taagtcaaaa acggatttat aggtacgaaa gccgaccgtg ttctgattgc ctggcggaat
    38461 ttcccccgtt tcaatcggac caccttcagc cgcgccccaa aacagccctt tacgtgctgt
    38521 ccaacgtgac ttgggatctg aatactctgc ccacgattgc ggcgtacttg gcacgacatg
    38581 gttgttattc gcaccaataa gttcagtcat catgtaatcg ttgagacgct tcatctcctg
    38641 acgatcggca atgttgaccc ctttcgtccc ttcaaccgca atcgctttca ggtttttcga
    38701 acccattatt gcgccagttc ccgcaccgcc gctgtggtta cggctattca acatgccaga
    38761 gagaggaaca aggttttccc cagcctgacc aatagccgcc acacaggttt ccggactggt
    38821 caatcgacaa atttcttccg tcgttgcgcg cgtccctttt ccccataaga aatcggcttt
    38881 ttccaggcta actttgtcat ctttaatctt cagccatacc ggtgatttcg ccttcccttc
    38941 gataataatg acgtcgtatc cagcgaattt catttgcgct gcaaaaaagc cacccatatg
    39001 ggcatcgacg actaaatttc ctttggtaaa agtagaaagt gaggtgatat ttacgcgaga
    39061 actacagggg gcaccagatc cagttaatgg gccggtagca aagactaatt tattcgcttc
    39121 atcgaaaggt ttcgtgcctg gcggtacttc gtcatacata attttgtagc cgaagcccat
    39181 gccaccgaca aaacttttaa acttactgga atcttcgagg gtaatatttc ctgtcgtgag
    39241 attgactctt aatatattac ctgtccaacc gttagccatg attttttcct ttgcaagatt
    39301 cacacagtaa tatctttcca ctcgataatt tttaacgccc ctgttgggca ggcgtttgcg
    39361 cattcaccgc ataacacaca tttcgaggat tttttacttt cggtatttac ggtggccatc
    39421 atccacgggc atgccgtagt acaggcgcta cagccaatac agcgtttatg atcgacggta
    39481 atacagcctt ctttctgctg ccaggtaatc gcaccaatcg ggcagacgtt catgcattgc
    39541 ggctctttgc attgacggca ggtgtccgcg gtatagttga gatcgccata caggccgccg
    39601 ccagagccaa ccccgttgtc gccaaagaaa taattgcgat ggattttgat acgggagaag
    39661 aatgtcccta ctgagccatc gttgaagttg gtacaagaga tttcacagcg gtggcaaccc
    39721 gtgcatcgcg ctcgctgggt caccagcacc cctttgggtg tatttatcaa ccctactgtg
    39781 ccgctatcaa tatcttcctg tttgcaaccc aaaagtgaca gtaacgcagg ggcaattgtt
    39841 agaccagcaa ggccttttcc tgatatacgc aagaattcta atcgcgttaa gccaatatct
    39901 aatagtggac gatcaaccgg gttcatttat ttcgcatcct cttcgcaaac acagtgctga
    39961 caattcagca gcctgatatt aagcattcaa ctaatacgct gctgactctg tccccaacac
    40021 cagatatttt ccctaacccg atagtgtatg agaaaagatt taacgccgat aaaccaatcc
    40081 tttatggtta ggagaaaaaa taatgtg
//
LOCUS       DAZPLI010000022        39890 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZPLI010000022 DAZPLI010000000
VERSION     DAZPLI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39890
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.022"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(128..803)
                     /locus_tag="ACHUU3_001096"
                     /pseudo
     CDS             complement(128..803)
                     /locus_tag="ACHUU3_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(948..1556)
                     /locus_tag="ACHUU3_001097"
     CDS             complement(948..1556)
                     /locus_tag="ACHUU3_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR2081231.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1740..2057)
                     /gene="metJ"
                     /locus_tag="ACHUU3_001098"
     CDS             complement(1740..2057)
                     /gene="metJ"
                     /locus_tag="ACHUU3_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR2081232.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2334..3494
                     /gene="metB"
                     /locus_tag="ACHUU3_001099"
     CDS             2334..3494
                     /gene="metB"
                     /locus_tag="ACHUU3_001099"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR2081233.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAVGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3497..5929
                     /gene="metL"
                     /locus_tag="ACHUU3_001100"
     CDS             3497..5929
                     /gene="metL"
                     /locus_tag="ACHUU3_001100"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR2081234.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6068..6202)
                     /locus_tag="ACHUU3_001101"
     CDS             complement(6068..6202)
                     /locus_tag="ACHUU3_001101"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2081235.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6278..7168
                     /gene="metF"
                     /locus_tag="ACHUU3_001102"
     CDS             6278..7168
                     /gene="metF"
                     /locus_tag="ACHUU3_001102"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR2081236.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7497..9677
                     /gene="katG"
                     /locus_tag="ACHUU3_001103"
     CDS             7497..9677
                     /gene="katG"
                     /locus_tag="ACHUU3_001103"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR2081237.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9771..10676
                     /gene="yijE"
                     /locus_tag="ACHUU3_001104"
     CDS             9771..10676
                     /gene="yijE"
                     /locus_tag="ACHUU3_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR2081238.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(10703..11320)
                     /locus_tag="ACHUU3_001105"
     CDS             complement(10703..11320)
                     /locus_tag="ACHUU3_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFR2081239.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11594..12697)
                     /gene="gldA"
                     /locus_tag="ACHUU3_001106"
     CDS             complement(11594..12697)
                     /gene="gldA"
                     /locus_tag="ACHUU3_001106"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFR2081240.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12708..13370)
                     /gene="fsa"
                     /locus_tag="ACHUU3_001107"
     CDS             complement(12708..13370)
                     /gene="fsa"
                     /locus_tag="ACHUU3_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR2081241.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13382..15883)
                     /gene="ptsP"
                     /locus_tag="ACHUU3_001108"
     CDS             complement(13382..15883)
                     /gene="ptsP"
                     /locus_tag="ACHUU3_001108"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR2081242.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16192..17271
                     /locus_tag="ACHUU3_001109"
     CDS             16192..17271
                     /locus_tag="ACHUU3_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR2081243.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17286..17606
                     /locus_tag="ACHUU3_001110"
                     /pseudo
     CDS             17286..17606
                     /locus_tag="ACHUU3_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            17657..19953
                     /locus_tag="ACHUU3_001111"
                     /pseudo
     CDS             17657..19953
                     /locus_tag="ACHUU3_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            19919..20797
                     /locus_tag="ACHUU3_001112"
     CDS             19919..20797
                     /locus_tag="ACHUU3_001112"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR2081244.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20799..21140
                     /locus_tag="ACHUU3_001113"
     CDS             20799..21140
                     /locus_tag="ACHUU3_001113"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR2081245.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21127..21978)
                     /locus_tag="ACHUU3_001114"
     CDS             complement(21127..21978)
                     /locus_tag="ACHUU3_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR2081246.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22193..23926)
                     /locus_tag="ACHUU3_001115"
     CDS             complement(22193..23926)
                     /locus_tag="ACHUU3_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR2081247.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24109..26760)
                     /gene="ppc"
                     /locus_tag="ACHUU3_001116"
     CDS             complement(24109..26760)
                     /gene="ppc"
                     /locus_tag="ACHUU3_001116"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR2081248.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27112..28263)
                     /gene="argE"
                     /locus_tag="ACHUU3_001117"
     CDS             complement(27112..28263)
                     /gene="argE"
                     /locus_tag="ACHUU3_001117"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR2081249.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            28417..29421
                     /gene="argC"
                     /locus_tag="ACHUU3_001118"
     CDS             28417..29421
                     /gene="argC"
                     /locus_tag="ACHUU3_001118"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR2081250.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29429..30205
                     /gene="argB"
                     /locus_tag="ACHUU3_001119"
     CDS             29429..30205
                     /gene="argB"
                     /locus_tag="ACHUU3_001119"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR2081251.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30266..31639
                     /gene="argH"
                     /locus_tag="ACHUU3_001120"
     CDS             30266..31639
                     /gene="argH"
                     /locus_tag="ACHUU3_001120"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR2081252.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            31906..32823
                     /gene="oxyR"
                     /locus_tag="ACHUU3_001121"
     CDS             31906..32823
                     /gene="oxyR"
                     /locus_tag="ACHUU3_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR2081253.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(32806..34206)
                     /gene="sthA"
                     /locus_tag="ACHUU3_001122"
     CDS             complement(32806..34206)
                     /gene="sthA"
                     /locus_tag="ACHUU3_001122"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR2081254.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            34527..35177
                     /gene="fabR"
                     /locus_tag="ACHUU3_001123"
     CDS             34527..35177
                     /gene="fabR"
                     /locus_tag="ACHUU3_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR2081255.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            35177..35536
                     /locus_tag="ACHUU3_001124"
     CDS             35177..35536
                     /locus_tag="ACHUU3_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR2081256.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(35576..36676)
                     /gene="trmA"
                     /locus_tag="ACHUU3_001125"
     CDS             complement(35576..36676)
                     /gene="trmA"
                     /locus_tag="ACHUU3_001125"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR2081257.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      36790..36980
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            37045..38889
                     /gene="btuB"
                     /locus_tag="ACHUU3_001126"
     CDS             37045..38889
                     /gene="btuB"
                     /locus_tag="ACHUU3_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR2081258.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            38834..39691
                     /gene="murI"
                     /locus_tag="ACHUU3_001127"
     CDS             38834..39691
                     /gene="murI"
                     /locus_tag="ACHUU3_001127"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR2081259.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9101 a        10032 c        11004 g         9753 t
ORIGIN      
        1 ataatatatg tcatttgaga aaaaagagca gaatgaggta aaaatctttg gctgtgtatt
       61 cagtctggta atcatccaag gaaagcttat cagaagggtg atgactccaa ttagtagaac
      121 atatattttc gataaaagca ccgatgactt tttcatgaag ttctacagaa cgcgagaaac
      181 agatggttct gcgggccaga cgtttgattc gggttctcag ggtcagattg ttacgctcaa
      241 tgtgctgagt aaaaatctta ccagtcagat gcttctcctt cggtaactct ctagcgtaac
      301 taccccattc gtcagtggtc aacatgccga tggtaaaagg gtaagcagcg cccgcaattc
      361 acgacaggtt tcgtcagtcc gggggccaaa agtgtaggcc agaacaccac cagttttcgt
      421 gttgtacacg taccagagcc agtgctgtcg cgctttactg ccgacaaagc tccattgctg
      481 actaagtttg cagatgagtg ccacatcagc atgagcgacc ggggatgacg ttattcttcg
      541 tggcgagagt tttttaaagt tcggatgact gtattaatgc cgattttcag cgtacgggca
      601 gtatcgcgaa ccccggcacc gttgaaagtc atttcagcga tttggtcttt aactcccggt
      661 ttacggggct cgtaacagta actgagctga aatacccggt gacagtcacg gccgcggaat
      721 cgctcatgtc tttttgggtt ctgcccgtga cggtaaacct gaacggactg gcaacggggg
      781 caatgaaggt taactgtgac catgaaacaa ccttaaaagc gagtattata tccttaattc
      841 aactaattgg aggcatcacc gtttaccgga gcgacagctt gcggaaaggt cgtgcaacta
      901 caaagcaaca aacagactac ggcgggaatt atttttttca tcatccatta ccacgaaaac
      961 agcggccact ctttggcgac tgtggtgagt tgtggcgcgt cgaaaacggc ttgtggtgaa
     1021 actaccgttt cctcaagcgg aatatttttg ccgtagcgtt ctttgagctt agcctggacc
     1081 agcgggttgc tgagatcgaa ctctttcaac ttctgctgct cgcccacgcg cattcccagc
     1141 gcgttctggt aaaccttcca caccacttcc gaacagtact ggcgatcgtc gctccatgag
     1201 aagctgaaat catacggttt accgagataa cgttttgccg tttgcgccag tttttgctgt
     1261 tgttcaacac tcagtccgcc ttcaacgcgg cgaacaacgt atttgccctt ttcaccatgg
     1321 gcgatccact gcttgagcgg ggtgtatttc accgggccga ccgcttcaaa aacgtagggc
     1381 tttttgttgc gtatcaccag cataccggtg tggctataat cggaatgggt cgccagttgg
     1441 atcgctttac tttgcgatga gcgagagatc tgaaagatga tgtcgccggt ttgtggctgc
     1501 caggcaaagg caggtacaga aaccagcagg ctgaggatca gcagcctgtt tttcatagtt
     1561 aaacgtccat gtataaaaag cggtgggtcg cagacaacgt gctcgttgtt tatgccggat
     1621 gcggcgtgaa cgccttatcc ggcctacaag ttcgtgcaaa ttcaataaat tgcaatatga
     1681 cgtaggcctg ataagcgtag cgcatcaggc gattccactc cgcgccgctc ttttttgctt
     1741 tagtattccc acgtctccgg gttaatcccc atctcacgca tgatctcttt tgccgcttcc
     1801 gggatttcgt cgctgcgctc tttacgcaga tcggcatcat ccggcaaagg ttgcccggta
     1861 aaggcatgca gaaacgcttc gcacagcagc tcgctgttgg tagcgtgacg caggttgttc
     1921 acctgacgac gcgtgcgttc atcggtgagg atttttaaca ccttaagagg aatggaaacc
     1981 gtaatctttt tgacttgttc actcttcttg ccgtgctcag cgtatgggct gatatattcg
     2041 ccgctccatt cagccatgag atacttaatc ctcttcgtca ataaattgag accagaccac
     2101 acaatttatg tgggtactga ccgtaaaccc gcatagttta ccgtacaggc gttaccgtga
     2161 catcgtgtaa tgcacctgtc ggcgtgataa tgcatataat tttaacggct atttgggatt
     2221 tgctcaatct atacgcaaag aagtttagat gtccagatgt attgacgtcc attaacacaa
     2281 tgtttactct ggtgcctgac atttcaccga caaagcccag gaaacttcat cacatgacgc
     2341 gtaaacaggc caccatcgca gtgcgtagcg ggttaaatga cgacgaacag tatggttgcg
     2401 ttgtccctcc catccatctt tccagcacct ataactttac cggatttaat gaaccgcgcg
     2461 cgcatgatta ctcgcgtcgc ggcaacccaa cgcgcgatgt ggttcagcgt gcgctggcag
     2521 aactggaagg cggtgctggc gcagtactta ctaataccgg catgtccgcg attcacctgg
     2581 taacgaccgt ctttttgaaa cctggcgatc tgctggttgc gccgcacgac tgctacggcg
     2641 gtagctatcg cctgttcgac agtctggcga aacgcggttg ctatcgcgtg ttgtttgttg
     2701 atcaaggcga tgaacaggca ttacgggcag cgctggcaga aaaacccaaa ctggtactgg
     2761 tagaaagccc aagtaatcca ttgttacgcg tcgtggatat tgcgaaaatc tgccatctgg
     2821 caagggaagt cggggcggtg agcgtggtgg ataacacctt cttaagcccg gcattacaaa
     2881 atccgctggc attaggtgcc gatctggtgt tgcattcatg cacgaaatat ctgaacggtc
     2941 actctgacgt ggtggctggc gtggtgattg ctaaagaccc ggacgttgtc actgaacttg
     3001 cctggtgggc aaacaatatt ggcgtgacgg gcggcgcgtt tgacagctat ctgctgctac
     3061 gcgggttgcg aacgctggtg ccgcgtatgg agctggcgca gcgcaacgcg caggcgattg
     3121 tgaaatatct gcaaacccag ccgttggtga aaaaactgta tcacccgtcg ctgccggaaa
     3181 atcaggggca tgaaattgcc gcgcgtcagc aaaaaggctt tggcgcaatg ttgagttttg
     3241 aactggatgg cgatgagcag acgctgcgtc gtttcctgag cgggctgtcg ttgtttacgc
     3301 tggcggaatc attaggggga gtggaaagtt taatctctca cgccgcaacc atgacacatg
     3361 caggcatggc accagaagcg cgtgctgccg tcgggatctc cgagacgctg ctgcgtatct
     3421 ccaccggtat tgaagatggc gaagatttaa ttgccgacct ggaaaatggc ttccgggctg
     3481 caaacaaggg gtaaaaatga gtgtgattgc gcaggcaggg gcgaaaggtc gtcagctgca
     3541 taaatttggt ggcagtagtc tggctgatgt gaagtgttat ttgcgtgtcg cgggcattat
     3601 ggcggagtac tctcagcctg acgatatgat ggtggtttcc gccgccggta gcaccactaa
     3661 ccagttgatt aactggttga aactaagcca gaccgatcgt ctctctgcgc atcaggttca
     3721 acaaacgctg cgtcgctatc agtgcgatct gattagcggt ctgctacccg ctgaagaagc
     3781 cgatagcctc attagcgctt ttgtcagcga tctggaacgc ctggcggcgc tgctcgacag
     3841 cggtattaac gacgcagtgt atgcggaagt ggtgggccac ggggaagtat ggtcggcacg
     3901 cctgatgtct gcggtactta atcaacaagg gctgccagcg gcctggcttg atgcccgcga
     3961 gtttttacgc gctgaacgcg ccgcacaacc gcaggttgat gaagggcttt cttacccgtt
     4021 gctgcaacag ctactggtgc aacatccggg caaacgtctg gtggtgaccg ggtttatcag
     4081 ccgtaacaac gccggtgaaa cggtgctgct ggggcgtaac ggttccgact attccgcgac
     4141 acaaatcggt gcgctggcgg gtgtttctcg tgtaaccatc tggagcgacg tcgccggggt
     4201 atacagtgcc gacccgcgta aagtgaaaga tgcctgtctg ctgccgttgc tgcgtctgga
     4261 tgaagccagc gaactggctc gtctggcggc tcccgttctt cacgcccgta ctttacagcc
     4321 ggtttctggc agcgaaatcg acctgcaact gcgctgtagc tacacgccgg atcaaggttc
     4381 cacgcgcatt gaacgcgtgc tggcctccgg tactggtgcg cgtattgtca ccagccacga
     4441 tgatgtctgt ttgattgagt ttcaggtgcc cgccagtcag gatttcaaac tggcgcataa
     4501 agagatcgac caaatcctga aacgcgcgca ggtacgcccg ctggcggttg gcgtacataa
     4561 cgatcgccag ttgctgcaat tttgctacac ctcagaagtg gccgacagtg cgctgaaaat
     4621 cctcgacgaa gcgggattac ctggcgaact gcgcctgcgt caggggctgg cgctggtggc
     4681 gatggtcggt gcaggcgtca cccgtaaccc gctgcattgc caccgcttct ggcagcaact
     4741 gaaaggccag ccggtcgaat ttacctggca gtccgatgac ggcatcagcc tggtggcagt
     4801 actgcgcacc ggcccgaccg aaagcctgat tcaggggctg catcagtccg tcttccgcgc
     4861 agaaaaacgc atcggcctgg tattgttcgg taagggcaat atcggttccc gttggctgga
     4921 actgttcgcc cgtgagcaga gcacgctttc ggcacgtacc gactttgaat ttgtgctggc
     4981 aggcgtggtg gacagccgcc gcagtctgtt gagctatgac gggctggacg ccagccgcgc
     5041 gttagccttc ttcaacgatg aagcggttga gcaggatgaa gagtcgttgt tcctgtggat
     5101 gcgcgcccat ccgtatgatg atttagtggt gctggacgtt accgccagcc agcagcttgc
     5161 tgatcagtat cttgatttcg ccagccacgg tttccacgtc atcagcgcca acaaactggc
     5221 gggagccagc gacagcaata aatatcgcca gatccacgac gccttcgaaa aaaccgggcg
     5281 tcactggctg tacaatgcca ccgtcggtgc gggcttaccg atcaaccaca ccgtgcgcga
     5341 tctgatcgac agcggcgata ctattttgtc gatcagcggg atcttctccg gcacgctctc
     5401 ctggttgttc ctgcaattcg acggtagcgt gccgtttacc gagctggtgg atcaggcgtg
     5461 gcagcagggc ttaaccgaac ctgacccgcg tgacgatctc tctggcaaag acgtgatgcg
     5521 caagctggtg attctggcgc gtgaagcagg ttacaacatc gaaccggatc aggtacgcgt
     5581 ggagtcgctg gtgcctgccc attgcgaagg cggcagcatc gaccatttct ttgaaaatgg
     5641 cgatgaactt aacgagcaga tggtgcaacg gctggaagcg gcccgcgaaa tggggctggt
     5701 gctgcgctac gtggcgcgtt tcgatgccaa tggcaaagcg cgtgtgggcg tggaagcggt
     5761 gcgtgaagac catccgctgg catcactgct gccgtgcgat aacgtctttg ccatcgaaag
     5821 ccgctggtat cgcgataatc cgctggtgat ccgtgggcct ggcgctgggc gcgacgtcac
     5881 cgccggggcg attcagtcgg atatcaaccg gctggcacag ttgttgtaat ttcagaaatt
     5941 taataatgcc cggtactcat gttttcgggt ttatggtttc taatgaaata tattgaatta
     6001 tcataggatt aggccggatt aagcgtttac gacgaatccg gcaagaagca ataagtacat
     6061 ggttagttta tatttgcagt ccggtttgct ttgcataccg gattttcttt ttcttaccat
     6121 cctgaagttt tttcatcttc cctgattttt cctcaccatc attggtcatt tttcggttga
     6181 cgcccttcgg cttttccttc atctttacat ctggacgtct aaacggatag atgtgcacaa
     6241 cacaacatat aactacaagc gattgatgag gtaaggtatg agcttttttc acgccagcca
     6301 gcgggatgcc ctgaatcaga gcctggcaga agtccagggg cagattaacg tttcgttcga
     6361 gtttttcccg ccgcgtacca gtgaaatgga gcagaccctg tggaactcca tcgatcgcct
     6421 tagcagcctg aaaccgaagt ttgtatcggt gacctatggc gcgaactccg gcgagcgcga
     6481 ccgtacgcac agcattatta aaggcattaa agatcgcact ggtctggaag cggcaccgca
     6541 tcttacttgc attgatgcga cgcccgacga gttgcgcacc attgcacgcg actactggaa
     6601 taacggtatt cgtcatatcg tggcgctgcg tggcgatctg ccgccgggaa gtggtaagcc
     6661 agaaatgtat gcttctgacc tggtgacgct attaaaagaa gtggcagatt tcgatatctc
     6721 cgtggcggcg tatccggaag ttcacccgga agcaaaaagc gctcaggcgg atttgcttaa
     6781 tctgaaacgc aaagtcgatg ccggagccaa ccgcgcgatt actcagttct tcttcgatgt
     6841 cgaaagctac ctgcgttttc gtgaccgctg tgtatcggcg ggcattgatg tggaaattat
     6901 tccgggaatt ttgccggtat ctaactttaa acaggcgaag aaatttgccg atatgaccaa
     6961 cgtgcgtatt ccggcgtgga tggcgcaaat gttcgacggc ctggatgatg atgccgaaac
     7021 ccgcaaactg gtgggcgcga atattgccat ggatatggtg aagattttaa gccgtgaagg
     7081 agtgaaagat ttccacttct atacgcttaa ccgcgctgaa atgagttacg cgatttgcca
     7141 tacgctgggt gttcgacctg gtttataaat agtgtggctt ttgtgaaaat cacacagtga
     7201 tcacaaattt taaacagagc acaaaatgct gcctcgaaat gagggcggga aaataaggtt
     7261 gtcagccttg ttttctccct cattacttga aggatatgaa gctaaaaccc ttttttataa
     7321 agcatttgtc cgaattcgga cataatcaaa aaagcttaat tacgatcaat ttgacctaca
     7381 tctctttaac caacaatatg taagatctca actatcgcat ccgtggatta attcaattat
     7441 aacttctctc taacgctgtg tatcgtaacg gtaacactgt agaggggagc acattgatga
     7501 gcacgtcaga cgatatccat aacacgacag ccactggcaa atgcccgttc catcagggcg
     7561 gtcacgacca gagcgcgggg gcgggcacaa ccactcgcga ctggtggcca aatcaactcc
     7621 gtgttgacct gttaaaccaa cattctaatc gttctaaccc gctgggtgag gactttgact
     7681 accgcaaaga attcagcaaa ttagattact acggcctgaa aaaagatctg aaagccctgc
     7741 tgacagaatc tcagccgtgg tggccagccg actggggcag ctacgccggt ctgtttattc
     7801 gtatggcctg gcacggcgca ggtacttacc gttcaatcga tggacgcggc ggcgcgggtc
     7861 gtggtcagca acgttttgca ccgctgaact cctggccgga taacgtaagc ctcgataaag
     7921 cgcgtcgcct gttgtggcca atcaaacaga aatatggtca gaaaatctcc tgggccgacc
     7981 tgtttatcct cgcaggtaac gtggcgctgg aaaactccgg cttccgtacc ttcggttttg
     8041 gtgccggtcg tgaagacgtc tgggaaccgg atctggatgt taactggggt gatgaaaaag
     8101 cctggctgac tcaccgtcat ccggaagcgc tggcgaaagc accgctgggt gcaaccgaga
     8161 tgggcctgat ttacgttaac ccggaaggcc cggatcacag cggcgaaccg ctttctgcgg
     8221 cagcagctat ccgcgcgacc ttcggcaaca tgggcatgaa cgacgaagaa accgtggcgc
     8281 tgattgcggg tggtcatacg ctgggtaaaa cccacggtgc cggtccgaca tcaaacgtag
     8341 gtcctgatcc agaagctgca ccgattgaag aacaaggttt aggctgggcg agcacttacg
     8401 gcagcggcgt tggcgcagat gccattacct ctggtctgga agttgtctgg acccagacgc
     8461 cgacccagtg gagcaactat ttcttcgaaa acctgttcaa atatgagtgg gtacagaccc
     8521 gcagtccggc tggcgcaatc cagttcgaag cggtagacgc accggaaatt atcccggatc
     8581 cattcgatcc gtcgaagaaa cgtaaaccga cgatgctggt gaccgacctg acgctgcgtt
     8641 ttgatcctga gttcgagaag atctctcgtc gtttcctcaa cgatccgcag gcgttcaacg
     8701 aagcctttgc ccgtgcctgg ttcaaactga cgcacaggga tatggggccg aaatctcgct
     8761 acatcgggcc ggaagtgccg aaagaagatc tgatctggca agatccgctg ccgcagccga
     8821 tctacaaccc gaccgagcag gacattatcg atctgaaatt cgcgattgcg gattctggtc
     8881 tgtctgttag tgagcgggta tcggtggcct gggcatctgc ttctaccttc cgtggtggcg
     8941 acaaacgcgg tggtgccaac ggtgcgcgtc tggcattaat gccgcagcgc gactgggatg
     9001 tgaacgccgc agccgttcgt gctctgcctg ttctggagaa aatccagaaa gagtctggta
     9061 aagcctcgct ggcggatatc atcgtgctgg ctggtgtggt tggtgttgag aaagccgcaa
     9121 gcgccgcagg tttgagcatt catgtaccgt ttgcgccggg ccgcgttgat gcgcgtcagg
     9181 atcagactga cattgagatg ttcgagctgc tggagccaat tgctgacggt ttccgtaact
     9241 atcgcgctcg tctggatgtt tccaccaccg agtcactgct gatcgacaaa gcccagcaac
     9301 tgaccctgac cgcgccggaa atgaccgcgc tggtgggcgg gatgcgcgta ctgggtgcca
     9361 acttcgatgg cagcaaaaac ggcgtcttca ctgaccgcgt tggcgtattg agcaatgact
     9421 tcttcgtgaa cttgctggat atgcgttacg agtggaaagc gaccgacgaa tcgaaagagc
     9481 tgtttgaagg ccgtgaccgc gaaaccggcg aagtgaaata cactgccagc cgtgcggatc
     9541 tggtatttgg ttctaactct gtcctgcgtg cggtggctga agtttacgcc agcagcgatg
     9601 ctcacgagaa gtttgttaaa gacttcgtgg cggcatgggt gaaagtgatg aacctcgacc
     9661 gtttcgacct gctgtaatct gaccttcttc agcgactgcc tttcaggcag tcgctgaagt
     9721 ttctttaccg gcgtatagtg tccacaggaa aactacacac tggatctctc atgtctgccg
     9781 caggaaagag caacccactg gcaatcagtg gcctggttgt gctcacactt atctggagtt
     9841 atagctggat tttcatgaag caagtcacca gctacatcgg tgccttcgac tttaccgcct
     9901 tacgctgcat tttcggcgct ctcgttttat tcatcgtcct tttattacgt ggtcgcggaa
     9961 tgcgcccgac accgtttaaa tacaccttag ccattgccct gttacaaacc tgcgggatgg
    10021 ttggcctggc gcagtgggcg ctggtcagcg gcggagcggg gaaggtggcg atcctaagct
    10081 ataccatgcc gttctgggtg gtgattttcg ccgcgttgtt tctcggtgaa cgcctgcgac
    10141 gtgggcaata tttcgcgata ctgattgccg ctttcggctt atttttggtg ttgcagccgt
    10201 ggcaactcga tttctcttcg atgaaaagtg ccatgctggc aatcctctcc ggcgtcagtt
    10261 ggggggcgag cgcgattgtt gctaaacgtc tgtatgcccg tcatccgcgc gtggatttat
    10321 tgtcgttaac atcctggcag atgctctatg cggcgctggt gatgagtgtg gtcgctttac
    10381 tggtgccgca acgtgaaatt gactggcagc ccaccgtgtt ctgggcgctg gcctacagtg
    10441 cgattctggc gacggcactg gcgtggagct tatggttgtt tgtattgaaa aacttgcctg
    10501 ccagtattgc cagcttaagc acactggccg ttcccgtttg cggcgtactc ttttcctggt
    10561 ggctgctcgg cgagaatccg ggggccgttg aaggtagcgg tattgtgctg attgtgatgg
    10621 cactggcgct ggtgagccgt aagaaaaaag aagccgttag tgtaaaaagg atctgaattt
    10681 tttcttcatg tggggcgatc gcttatttaa caaaataacg ataatgcccc accatgcgcc
    10741 agctaaacag cacatcttct tcctgcgcac ctgcgccaat gttatgtatc accagcggcg
    10801 taccgtcgcg ggcgaagcca tctgaaacca ccccaatatg tgccagcccg ttatctaatc
    10861 gccaggagac aatatcgccc gcttgatagt cactggggtt cttgctgatg gggcgtgttt
    10921 tatcgtggtg ggtaaaccag gtttccagat taggcacccg acggtgatcg atgttgctgt
    10981 ccgggcgctt taactgccat ttttgcgggt actcggcaaa attcttcgcc atatcttcgt
    11041 gcaccagttt ctgcaaatcg accttctggc tgcgcaatgc acggatcacc acatcggaac
    11101 ataccccgcg tttttgcggg acatcaccgc caggataggt aagctgcata tacgccggat
    11161 cgtaaaatag cgtgctgccg atttgctgtc tggcaccgtc tgcgatggca aggttggtat
    11221 tggcctggat ttgtaccacg gttggtggaa cggcgggaga ttttaaggag tggctggtaa
    11281 atgccgttag caggctgagc agcgccagtg aagctttcat ctggttcgtc cgtgaaaata
    11341 agagtcacag attcgacctt ccgggataac gattgttctg tagagaaaat gtccagaagc
    11401 ggataaaaaa ctcctgaaca acgtgggagt ttcagattac tggcacagag tgccggatgc
    11461 ggcgtgaacg ccttatctgt cctaaaaaac aagaaaattc aatatattgc agagattgcg
    11521 taggcctgat aagcgtagcg catcaggcaa ttttgcgttc aaactcccgg acaagccggg
    11581 agtttggagt aggttattcc cactcttgca ggaaacgctg accgtactgg tcggctacca
    11641 gcagagcggc gtaaacctga tctggcgtcg cgccgccagg catgttgtgg atggtttcac
    11701 attctgcaca tgccgcttca gccacaattc gcattttcgc cgggacatct tctttaatat
    11761 ccagttgagc gagagttatt ggcaaaccta ccgcatggct aagtgcagct acggtttcga
    11821 tttcctcaac cggtgcgttt tccagaacca gctgcgtcag cgtaccaaat gccacttttt
    11881 caccgtgata atagtgatgc gcgtccggga tagcggtcag gccgttatgc actgcgtgcg
    11941 ccgcagccag accaccactt tcaaaaccaa caccgctcaa ataggtgttc gcttcaatca
    12001 cgcgctccag cgccggagtc actacatgct gttcggcagc aagcatcgct ttttcgcctt
    12061 cttccagcag ggtgttgtag cacagttcag ccagtgccag cgcagcctgg gtgcacttgc
    12121 cgcccgccat ggtggtcgcg ccgctacgag agcaggcacg cgcttcaaac caggttgcca
    12181 gcgcatcgcc gatacccgcc gctaacagac gtgcaggtgc gccagcgacg attttggtgt
    12241 cgacaatgac catattcggg ttatttggca acagcagata gcggtcaaac tcaccctcat
    12301 cggtgtagat aacagacaat gcgctgcacg gtgcatcggt agaggcgata gtcggtgcga
    12361 tcgctaccgg aacacccatg aaatgtgcca gtgctttggc tgtatcgagg gtttttccgc
    12421 caccgatacc gagaattgcg ccacactgcg cagtctccgc gatgccacgc agacggtcga
    12481 tctcattttg cgaacatcca ccgccaaacg gcgcaatttc tactaccagt ccagcatctt
    12541 taaagctttt ctcgacagtg gattgagcaa aacctaaaac aaatttgtca cccaccacta
    12601 accagcgttc tgccagcggc ttcaggtatt cgcccagacg attaatcaca tcagcgccct
    12661 ggatgtattt acccggtgat tgaataatgc ggtccataat tgctccttta gagatgagta
    12721 gtgccaaatg cggcattcca gtcgtgttcg aacttctcta tagctgactc taccgcaggg
    12781 gtgttgagca tttgttgcgc tacatctaag ggcagggtga tggattcaca tcctgccagt
    12841 aaacagtcca gcgcctgacg cggcgtttta aagctggctg ccagcaccat gctttctggc
    12901 gcgtgcattt ctaacagcgc ttgcagctcc tgaaccgtac gaatgccgtc tccgccctgg
    12961 gcatctacgc ggttaacata cggagcaacg tattttgccc ctgccagcgc ggccagtaac
    13021 ccttgtgcag cactgtacac tgcggtgcca agcgtagtaa taccctcttt tttcagtatt
    13081 ttaattgctg ccagaccttc ggaagtcacc gggattttca ccacaatacc cggaatagcg
    13141 tcgcgcaggc gcttcgcttc ttccaccatc ccctgcgcgt cgcggctcat ggtctgagca
    13201 aacagaatgc cctcatcacc aatcgctttt tgcagacgcg gcagcacttc ccatatggac
    13261 tccttgctgg cagcgataat gctcgggtta gttgtcaccc cggcaatggg gaatatgcgt
    13321 gccagacgtt cgacttctgc gacgttagcg gtgtccagat acagttccat gatgtttcct
    13381 tttacagttc cagttcatgt tgcagcaggc tggcgatagc gtcggcagag gcggcgttaa
    13441 ccagcgcgtt acggaattct tcgtgcatga tgcggcgagc gaggcgcgag aaaatgcgca
    13501 tgtgctgatc gcccgcagcg tgtttgttca gggttaacat gatgatgaat tgcgcttcat
    13561 catcacccca gcgcaccggc gcttgcagac gcgcaacgct gatggtggat tgctcaatgt
    13621 gttctgattt gctgtgtgga atggcaaaac taaagcccag accggtagag aaaacggcct
    13681 cgcgcgccca caagtcggct tccagtttac gcggatagcg gcagcgccct gccagcagca
    13741 ggttatcggt catgcctttg agcacttctt ctttgctgcg ccagtcgctt tccagtgtga
    13801 tgcactcggc ggtgaccagc ggtgcgtctt gttgggtcat gcggaattgc gccagcaggt
    13861 gctccacttc cagcgaagta cggcaggcca ttgcctggtt gagcaacttg cggcactcac
    13921 ggctatcaag ttgcgccatc cgcgctttcg ccgccggaat tgatggtgcg ctcatgctga
    13981 gttcatccag ccctaagccg accagcaacg gcagcacgga acctttcgct cccagctcac
    14041 cgcacagacc aatccattta ccctggcgat gcaccgcctg cacggcgtaa tcgagcgccc
    14101 gcaagaatgc cggattcagg ctgttgtagt gacgagtaac cttagcgtta tcgcgatcca
    14161 ctgccagcag atattgcgtc aggtcattac taccaatact aaagaaatca atctcttcgc
    14221 agcattgatc gatgatgaac atcaccgacg gcacttccag cataatgccg agctggatct
    14281 tctcatcaaa cggaatgtgt tcgttacgta gttgctgttt ggcttctgcc agtttttctt
    14341 tcacccataa gatctcttcc attgaggaga tcatcgggat catgattttc aggctgccgt
    14401 gagcggaggc gcgcaggatc gagcgtagtt gtgtggtaaa taacgacgcg tattcttcat
    14461 aaatacgcac ggcgcgataa ccgaggaacg ggtttgcctc tgcgggaatg tttagataat
    14521 caacgggttt gtcgccgcca atgtccatag tgcgcacaat aatgctgcgt ccgttggcag
    14581 actccagcgc ctgacaaaaa atgttgtaca gctcgctttc gcccggtgca ctggtgcgat
    14641 ccatatagag catttcagtg cggaacaaac caacgccttc cgcaccattg ccgaatgcgg
    14701 cctgcgcttc cacggagtga gcgatgttag cggcaatttc aatgcggata ccgtcagcgg
    14761 tacgggcttg ttgggtcagc cagacacgct gttgctcacg cagggcgtcc tgtacgcggg
    14821 cttcttgctg ataataacga gctacggctt ctcctggctc aaccacaatc gccccggcgt
    14881 tgccgtcgat ataaatcgtt tgatgctgcc acggagtgag ggcatcaata tccacaccaa
    14941 ccagcgtcgg aatgttgaac gaacgggcaa ggatcaccgt atgtgaggtg gtgccgccac
    15001 ttttcagcaa cagacctttg aggtgatttt tatccagttc gaggaactgg ctgggggtca
    15061 gttcatcagc catacaaatg gcggactgcg tcagtttgcc cggtgccggg aagcgttgct
    15121 caccgtagat ttgctggagt aactggaagc agacgtcgcg tacgtccagg gcacgttctt
    15181 gcaggtagct gctgctggaa cgggcgaact cttcgcaaaa gtgattcgca ctggcgacaa
    15241 tcgcttcggc gcagcttaat ccctcgctga cgcctgccag taagtgctga cgtaaagatg
    15301 tatcgccagc cagggatcgg tgagcttcca gtatcgcgct ggtagcaccg tcgctatcca
    15361 gcagacgaaa ctcaatgttt ttcagtacca gcgtcaggcc gttttccagt gtggattgct
    15421 cggcgtcaac gtctttggct gcgggaagat tactcagcgc attgagatct aaagaagaga
    15481 tcggcgtcag aatgccgcct gcactaccgc tgcacacggt gcgggcgcgg ataatttgcg
    15541 gattcagatt ggtcagtgaa accggcagtg gttccagttc gtcagattta acttccgcca
    15601 gcggcgcatc gcagtggggg aattcatcgc gcagccattg gcttaaacgc tggtgcgctt
    15661 cctgttcgtc ggccccggaa atcagtaact ggcagttatc gcccgccagc gtatcggtgc
    15721 caatcagcgc aagggcgctt ttggcgttgc ccttgcggtc agtgtgcagg ttatgccact
    15781 caatttgtga tgaaaaagta ttacacagcg tttcaacgtg gcttgccgga cgcgcatgta
    15841 cgccgttagg tagctcacaa ataaattcca caatcagggc cattgcctct ctcccggtac
    15901 ggttttctgt ttacaggata agagcgcacg gcaacggcct gccatgtgac aaatctgcca
    15961 aaagctggac aaatgtaatg taaccgtcaa tttgcgacgc gtctcacaag acgccgtttt
    16021 gcggcatgct tccggtttat cgcaagttat gaggcggatc gcatttttgt actgatatta
    16081 caaaagtcca gtaaatggcc tttttatcca ctgtttgctc cgcctgcgat tgcctattgt
    16141 ttcctccatc aaaaatatgg acatgggctt tcgcaagccc gggagcagct tatgaatgag
    16201 ttggtgcaga tcctgaaaaa tacccgtcag catttaatga cgggcgtttc acacatgatt
    16261 cccttcgtgg tatcgggcgg tattttgctg gcggtttccg tcatgttgta tggcaaaggc
    16321 gcagtgccgg atgccgtagc cgatccaaat ctgaaaaaac tgtttgatat cggcgttgcg
    16381 ggcttgacgc tgatggtgcc tttcctcgcc gcttacattg gttattccat tgcagaacgt
    16441 tctgcgctgg ctccatgcgc tatcggtacc tgggttggta acagctttgg tgcgggcttc
    16501 tttggtgcac tgatcgccgg gattatcggc ggcatcgtgg tgcattacct gaagaaaatt
    16561 ccggtgcata aagttctgcg ctcggtgatg cctatcttca ttattcctat cgtcggcaca
    16621 ctgattaccg caggcatcat gatgtggggc ttgggcgagc ctgtaggggc gttgaccaac
    16681 agcctgactc agtggcttca ggggatgcag cagggcagca ttgttatgct ggcggtgatc
    16741 atgggtctga tgctggcgtt cgatatgggc ggtccggtta acaaagtggc ctatgccttc
    16801 atgctgattt gcgttgctca gggtgtttat accgtggtgg ccatcgctgc cgtgggtatt
    16861 tgtatcccgc cgctggggat ggggctggcg acgctgattg gtcgtaaaaa tttctccgca
    16921 gaagagcgcg aaaccggcaa agcggcactg gtgatgggct gtgttggcgt tactgaaggg
    16981 gcgattcctt tcgccgctgc cgatccgctg cgtgtcattc cttccatcat ggtcggttca
    17041 gtttgtggtg cagtaactgc ggcgctggtc ggtgcgcagt gctatgcagg ctggggtggt
    17101 ctgattgtgc tgccggtggt tgaaggcaag ctgggttata tcgcagcagt ggctgtcgga
    17161 gcagtggtga cggctgtttg tgtgaacgtg ctgaaaagtc tggcgcgtaa aaatgggtct
    17221 tcgactgatg aaaaagaaga cgacctggat ttggattttg aaattaatta attgaggaac
    17281 gagccatgac gaaaattatt gcagtaaccg catgtccttc aggtgttgcc catacctata
    17341 tggcagcaga ggcgctggaa agtgccgcga aagcgaaagg ctgggaagtg aaagtagaaa
    17401 cctaggggtc aattggtctg gaaaatgaac tgactgcgga agatgtggcg agcgctgata
    17461 tggtgattct gaccaaagat atcggcatca agtttgaaga gcgttttgct ggcaagacca
    17521 tcgtgcgcgt caacatcagc gatgcagtaa aacgtgctga cgccattatg agcaaaattg
    17581 aagctcacct ggcgcaaacc gcttaagttc ttctcccgct cgcaagggcg ggttcgcttt
    17641 cccacaggag ttcctcatga cgaatcgtat ctctcgcctc aaaactgcac tgtttgccaa
    17701 tacccgtgaa atctcgctgg agcgggcgct gctttatacc gccagccatc ggcaaaccga
    17761 aggcgaaccg gtgatattgc gccgggcgaa agcaacagcg tatatccttg aacatgttga
    17821 aatttcgatt cgtgatgaag aactgattgc cggtaaccgc accgtaaaac cgcgcgccgg
    17881 gattatgtcg ccggaaatgg acccttactg gctgctgaaa gagctggatc aattcccgac
    17941 gcgtccgcag gaccgctttg ctatcagcga agaagataaa cgtatctacc gtgaagagtt
    18001 gttcccgtac tgggaaaaac gttcgatgaa agatttcatc aacgggcaga tgacagatga
    18061 agtaaaagcc gcgaccaaca cgcagatttt cagcatcaac cagacggata aaggccaggg
    18121 gcacattatt attgattacc cacgcctgtt gaatcacggt ctgggtgagc tggtggcaca
    18181 gatgcagcaa cattgtcagc aacagccgga gaatcacttt tatcaggccg cgttgttact
    18241 gctggaagcc tcgcagaaac acattttgcg ttacgccgaa ctggcggaaa cgatggcggc
    18301 aaactgcaca gatgcacagc gtcgcgaaga actgctgact attgcggaga tctctcgcca
    18361 taacgccgaa cataagccgc agacgttctg gcaggcgtgc cagttattct ggtacatgaa
    18421 catcattctg caatacgaat ccaacgccag ttcgctgtct ttagggcgct tcgaccagta
    18481 tatgttgccg ttctatcagg catctttaac ccagggcgaa gatccggcgt tcctgaaaga
    18541 actgctcgaa tctttatggg tgaaatgcaa cgacatcgtg ctgttgcgct ccaccagcag
    18601 cgcgcgttat ttcgcaggtt tcccgaccgg ctataccgca ctgctcggcg ggttaaccga
    18661 gaacggacgt agcgcggtga acgtgctttc gttcctttgc cttgacgcct atcaaagcgt
    18721 gcaattaccg caaccgaacc tcggcgtgcg cactaacgcc ttgatcgaca cgccgttcct
    18781 gatgaaaacc gccgaaacca ttcgcctcgg caccggtatt ccgcaaatct ttaacgatga
    18841 agtggtggta ccagcgttcc tcaaccgtgg cgtttcgctg gaagatgcgc gcgactattc
    18901 cgtagtgggc tgtgtggaat tatctattcc cggcagaacc tacggcttgc atgacatcgc
    18961 gatgtttaat ctgctgaaag tgatggaaat ctgcctgcat gaaaatgaag gcaatgctgc
    19021 gctgacttat gaaggtttac tggagcagat ccgcgccaag atcagccact acatcaccct
    19081 gatggttgag ggcagcaata tttgtgatat cggccatcgc gactgggcac ctgtaccgct
    19141 gctctcgtct tttatcagcg attgtctgga aaaaggtcgc gatattaccg atggcggcgc
    19201 gcgttataac ttctccggcg tacaggggat cggtatcgcc aacctgagcg attctctcca
    19261 tgcgttgaaa gggatggttt ttgagcaaca gcgtttaagt tttgacgaat tgctgtcggt
    19321 attaaaagcc aactttgcaa cgccagaagg cgaaaaagtc cgtgctcgct taattaaccg
    19381 cttcgagaaa tacggtaacg atatcgacga ggtggataac atcagcgccg aactgttacg
    19441 ccactactgc aaagaagtgg aaaaatacca gaacccgcgc gcggttactt cacgccggga
    19501 tcgtataccg tttctgctca cgtcccgttg ggatcggtgg ttggcgcgac gccagacggt
    19561 cgttttgccg gggaacaact ggcagacggc ggcttgtcac ctatgctggg ccaggacgca
    19621 caagggccaa cagcggtact gaagtcagtc agtaagctcg ataatacgct gctgtctaac
    19681 ggtacgttgc tgaacgtgaa attcactccg gcgaccctgg aaggtgaagc gggattacgc
    19741 aaactggccg acttcttacg ggcgtttacc cagcttaagt tgcagcatat tcagtttaac
    19801 gtggtgaacg ccgacacgtt gcgggaagcg caacagcgcc cacaagatta tgccgggctg
    19861 gtggtgcgcg ttgccggata cagcgccttc tttgtcgaac tgtcgaagga gatccaggat
    19921 gacatcatcc gccggacagc gcatcagctg taacgttgtg gaaacgcgcc gcgatgatgt
    19981 ggcgcgcatt ttcaacattc agcgttattc actgaatgac ggcgagggca ttcgtacggt
    20041 ggtctttttt aaaggctgtc cgcatctttg cccgtggtgt gctaatccgg agtcgatctc
    20101 cggcaaaatc cagacggtac gcagagaggc gaaatgtctg cactgtgcga aatgtttgcg
    20161 tgatgcggat gaatgcccct ccggggcgtt tgaacggatt ggtcgcgata tcagccttga
    20221 cgctctggaa cgggaagtga tgaaagatga cattttcttt cgcacgtccg gcggcggcgt
    20281 cacgctttct ggcggcgaag tgttaatgca ggcggagttt gctacccgtt ttttacagcg
    20341 actgcggctg tggggtgtgt catgcgccat tgaaactgcc ggagacgcgc cagcaagcaa
    20401 gctgttaccg ctggcgaaat tgtgcgatga agtgctgttc gatttaaaaa ttatggacgc
    20461 gactcaggcg cgggatgtgg tgaagatgaa cctgccacgc gtgctggaga atctgcgttt
    20521 gctggtgagt gagggtgtca acgtgatccc gcgtttaccg ttgatccctg gtttcacgct
    20581 cagcagggag aatatgcagc aggcgctgga tgtactgatc ccgctgaaaa tcaggcagat
    20641 ccatctgtta ccgtttcatc agtacggcga accgaaatac cgcctgctgg ggaaaacatg
    20701 gtcgatgaaa gaggtgcctg cgccgtcgtc agccgatgtg acaacgatgc gcgaaatggc
    20761 agaacgggcc ggatttcagg ttaccgtggg aggttaaaat ggcatacctg gtggcagtaa
    20821 ccgcctgcgt aagcggcgtg gcgcatactt atatggcggc ggaacggctg gaaaagttgt
    20881 gccagttaga gaagtggggc gtcagcattg aaactcaggg cgcgctggga acggagaatc
    20941 gtttagcgga cgaggatatc cgtcgggcgg atgttgctct gttgattacg gatatcgagc
    21001 ttgccggtgc cgagcgattt gaacattgcc gctatgtgca atgcagcatc tacgcattcc
    21061 tgcgtgagcc gcagcgggta atgagcgcgg tgcgcaaagt gctttctgcg ccgcagcaaa
    21121 cccatcttat tctggagtag tcggtttttc tgtcagttgg ctgtggtact gccggcgata
    21181 ttccgacggc gagcgttcgg tgtttttacg aaacagacgg cagaagtagt tgctgtcgac
    21241 aaagccgcag gcgtgcgcca cttcttttac cttcaggtca tagcctttca ataacgtctt
    21301 agcgtgctcc agtcgcgtgt gattcaggta ttcgttaaaa ccaatggccc ccgttttttg
    21361 aaacaggtgc gagagatagt ttggtgaaat atagaatgcc tgtgcgacag attcgcgggt
    21421 aagcgcggag gcatagcgtt cgtcgatata atcgcgaata gcttcaaata gtgcctggct
    21481 gcgtgaggcg gtctggattt ggctgccgag caaatcgcgg cagtggctaa gcaggctggc
    21541 tacgataagc cttgccgttt gctgctcctg cggctgcatt tgcatttcat tgagcgtttg
    21601 tagcagaaaa gaacctatac gtgggccgcg tcgggcgacg tgttgcttcg ccagattttg
    21661 atattgtttg ccatcccatt gcacgacgct gaagccgagt tgctgtttgc caaacagcac
    21721 gctaaaggtg gtagcgggtg ttttccattg tgggaaattc cagccaccag cggcaacgta
    21781 cagcacatcg ccgggaacta acgttgctcc agcgccggta tcaacaaact caccttccag
    21841 cacaatttcc agccgtggaa aatcgacctg atacgcaaga tcgggcaccg ggccgttact
    21901 gctggcaaag taaatctggc gcagggacag cgggccgttg atcaggcggg agagcagata
    21961 gctgacgtcg tgatacatgt tattccctta ggtatgtaga cgggaaaaca gtaactggca
    22021 ttattaaaaa aggccacctg aaaagtgtgg cctgagggga gttcgatgta aacattgcgg
    22081 cagttgtatt gccggatgtg gcgtaaacgc cttatccagc ctaccgccgc gatctgtagg
    22141 ccggataaga cgcgtcaagc gtcgcatccg gcaaatagcg cctggctgat aattactgat
    22201 tacctacctg gtcgccatac ggcagagtgt cgtaatcgat cagcgcgttt ttcttgtacg
    22261 ggttaccaat ccagcgggta gtttctttga actgcggatt caccactgaa cgggttggat
    22321 cgtaaccgtc gtaagataag cccgccaaat ctgaccaggt gtggatcagt tccgccaggc
    22381 tgtatttacg atcgacatcc tgtgagaaat cacggggatg agtcgcttgc catttttccg
    22441 acgtccacag caggaacgga atggtgtaca tgtggcgcgt cgggttgtct tcgttacgcc
    22501 cctgagtttt gtgcggcggc gtgtcgtaaa cctcttcacc gtggtcagag aagtaaacca
    22561 ggaatccgtt cgggtctgcc gctttaaagt ctttaatcag gctggcaacc acatgatcgt
    22621 tatacaggtt agcgttgtca taatcgttat atgattccag ctcttctgcg cttaatcctg
    22681 gcggaacatg atcggtattg ccatcaaact tgccctgatt ttccgggtag cggtatttgt
    22741 atttgatatg cgtacccagc agatgaacga tgatcagttt cttcggcgca gggtcattca
    22801 gcacatcctg gaacggcttc agcacgttgg tgtcgtattc acgcgcactc tgcgtacgtt
    22861 gctggttcat gtagtactgc ttgtcggtct ggcgcgaaaa taccgtcagc atggtattgc
    22921 gggcggtcat cgtctgctgg ttggtgatcc agaaggtttt ataacccgcc tgtttcatca
    22981 tgttcatcag cgacggctgc gtcagataca gatccgggtt cttttcattg gcaaaggtca
    23041 gcgcctgttg caggatttca atggtgtacg gacgagacgt aactacgtta ttaaacacgg
    23101 tcagattcgg atcggtttta tgcagcgcat ccagctccgg cgtggtttca cgcggataac
    23161 cgtacagact catgcgtccg cgctgggtcg actcgccaat caccagcact aaagtgcgcg
    23221 gttcgttacc cgattcatct ttgaaattag ccagtggcgg caaggcatta ttttcattca
    23281 gtaactttgt tagcgagttt agttgctgac gatactgata atagccggtc aggaattgcc
    23341 acggtgcggc aggctccata cgcgaggcca ggttatccaa cgttttctca aacggcttgt
    23401 ttttgataaa cgtattcatg gcgatcggat gcagaatcaa gccataaagc agggcaaaag
    23461 agacaacata acgccacggc tttggaatat agaccgggcg caggcgtgtc cacagcagaa
    23521 ctgccaccgc cgtataggcc agcgcgataa gcacaatttt caggctgaaa tactggctta
    23581 aatactcgct ggcttcgttg gtgttggttt cgaacatcac aaacagaacg ctctgcgaga
    23641 actcctgccc gtagatgacg tagtagcata gcgccgccag agaggccgcc catagcacca
    23701 cgccgattac tgcggcaata attttaatcc gcttcggaaa gaggaatacc gggatcaacc
    23761 acagcgagct gaataacagc gagtcgcgaa tgccgttggt gccactataa ccactgatgt
    23821 aaataatggc ctgtagcaga gtagagaaaa accaaaagta gagcagtgcc caacccaggg
    23881 ctttccagct aaaaaggggt ttagcctgga cttctgtgga atgcatagta agaacctgtc
    23941 ttgaaaaaaa tatcgccgaa tgtaacgaca attccttaag gatatctgaa ggtatattca
    24001 gaatttgaat aaaatgcaga cagaaatata ttgaaaacga gggtgttaga acagaagtat
    24061 ttcagaaaac cctcgcgcaa aagcacgagg gtttgcagaa gaggaagatt agccggtatt
    24121 acgcatacct gccgcaatcc cggcaatagt gaccattaac gcttgttcga cgcgaggatc
    24181 cggttcctgg ccttcttttt ctgcctggcg ggagcggtgc agcaactcgg cctgcaatac
    24241 gttcagcggg tcggtgtaaa tattccgtag ctgaatagac tctgcaatcc acggcagatc
    24301 ggccatcaga tgggaatcgt tggcaatcgc cagcaccact ttgatgtctt cttcttgcag
    24361 gttgcgtaac tctttaccta acggccacag tgctttgtct accagacgtt gatcatagta
    24421 ttccgccaac cacaggtctg ctttggcgaa gaccatctcc agcatgccga gacgcgtcga
    24481 gaagaatggc caatcgcggc acatggcttc cagctcgctc tgtttgccat cttcgaccac
    24541 tttttgcagc gccgtacctg cacccagcca ggcggggagc atcaggcggt tttgcgtcca
    24601 ggcaaaaatc cacggtatgg cgcgcagcga ctcgacgccg ccggttgggc gacgtttcgc
    24661 cggacgtgaa cccaacggca gtttgcccaa ttcttgttcc ggcgtagcgg agcggaagta
    24721 aggcacaaaa tctttgtttt cacgtacgta gccacggtag agatcgcagg agatgactga
    24781 cagttcatcc ataatgcgac gccagctctc tttcggctca ggtggtggca gcaggttggc
    24841 ttccagaatc gccccggtgt aaagcgacag gctgctgacg gtgatttctg gcagaccata
    24901 tttaaagcgg atcatctcgc cctgttcagt cacgcgcagg ccacctttca ggcttcctgg
    24961 cggttgtgac agcagcgccg catgggcagg tgcgccgccg cgaccaatgg aaccgccgcg
    25021 accgtggaac aacgtcagct caatacccgc tttttcgcag gttttgatta atgcatcctg
    25081 tgcctgatat tgcgcccagg aagctgccat cacgcccgca tcttttgctg agtcggaata
    25141 gccaatcatc accatctgtt tgccctgaat caggccgcga taccagtcga tattgagcag
    25201 ctgggtcatg acatcgttgg cgttgttcag gtcatcgagg gtttcaaaca gcggagcaac
    25261 cggcatcgca aacccgatac ccgcttcttt cagcagcagg tggacagcca gtacgtcgga
    25321 cggcgttttc gccatcgaga tcacgtaggc ggcaatggag ccttgcggcg cttcggcaat
    25381 cacctggcag gtatcgagca cttcgcgcgt ttcggcgctt ggttgccagt tgcgcggcag
    25441 aagcggacgt ttggagttca gttcgcggat caggaacgcc tgtttgtcgg cctctgacca
    25501 gctttcgtag tcgccgatac cgaggtagcg ggtcagctcg cccagcgctt cggtatggcg
    25561 ggtgctctcc tgacggatat caatacggac cagcggtacg ccgaaacatt tcacgcggcg
    25621 cagggtgtcg agcagatcgc cgttggcgat aatacccatg ccacacgcct gaagtgactg
    25681 gtagcaagcg tagagcggtt cccacagttc ttcgttttgt gtcagcagcc cttctggttt
    25741 tggcagttct tcgcctttca ggcgcgcttc cagccatgcc tgtgtcgcca tcaggcgaga
    25801 acgcaggttt ttcatcagat agcgatacgg ttctgcggca ccttcttcgc caaccagcgc
    25861 cagcagttca ggggtcgctt caaccatcga cagttcagaa accagaacct gaatatcttt
    25921 caggaacaga tcggtggctt tccagcgact gagtagcagg acgtggcggg tgatatcggc
    25981 agtgacgttc gggttgccgt cgcggtcacc gcccatccac gaagtaaaac ggaccggaac
    26041 aaattcgacg ggcagtttgt agccgaggtt ctcttccagt tgttcgttca gttcgcgcaa
    26101 gtaattcggt acgccttgcc acaggctgtt ttctactacg gcaaagcccc atttggcttc
    26161 atctaccggg cttggacgca gcttacggat ttcatcagta tgccatgact gggcgattaa
    26221 ctggcgcagg cgacgcatca gctggttgtg ttcgtagtca gcgatatctt tgttatcgag
    26281 ctgttttaag caggcgttca cttccaccat tttgtggatc agtgtacgac gggtaatttc
    26341 ggttgggtga gccgtgagga ccagttccag cgacagcgat tccactgctt ttttgatggt
    26401 gtcttcgctc agttccggct ggtttttcag tttgcgcagg gtgcgggcga tcacttccgg
    26461 gttgctggca gcttcgcctt tcggcgaaat gctgtggtat tgctcggcgg tgttggctag
    26521 gttcaggaac tgactaaacg cacgcgcaac gggcagcagc tcgtcgttcg acaaattttg
    26581 taaggtggtg agcaactcct ggcggttagc atcattgcca gcgcgtgaag atttcgacaa
    26641 cttacggata gtttctacgc gttcaagaat gtgttctccc aacgcatcct tgatggtttc
    26701 tcccagcact ttgccgagca tactgacatt actacgcaat gcggaatatt gttcgttcat
    26761 attaccccag acaccccatc ttatcgtttg atagccctgt atccttcacg tcgcattggc
    26821 gcgaatatgc tcgggctttg cttttcgtcg tcttttataa agccacgtaa aagcggtgac
    26881 gtcaaatgct gcgaaatcgc ttcagcaaac gaataaatag caggaattta cgccattaaa
    26941 ttcacgacgc tttaaataag cgtaacttat ggaaatgtta aaaaatcgcc atacataaca
    27001 ccaaaggtgt aggtcggata agatgcgcaa gcatcgcatc cgacattatt gcggcaccgg
    27061 agtttggcaa cggtgccgga tgcggcgcga gcgccttatc cggcctacgt tttaatgcca
    27121 gcaaaaatgg tgaattacct ggataatcaa ctcacgggtg ggtttaataa accgcgtctc
    27181 cagatattca tcaggctgat gagcttgatt gatggaaccc ggaccgagca ccagcgtcgg
    27241 gcataacgtt tgaataaacg gcgcttcggt acagtaattc accacttcgg tttttgctcc
    27301 gagcaatttc tcaaccactt caaccagttg atgattcggt gggcattcat agccagggat
    27361 cggcggatgc agctcgtcga ccgtcagacg acccggccag cgttcgctca ccggagccaa
    27421 tgcatcgttg agcaaaccat taagttcatt gagtgtcatg ccaggcagcg gacgaatatc
    27481 catatgcaac tcacagcaag cgcaaatacg gttagaagcg tcgccaccgt gaatatgccc
    27541 gaggttgagc gtagggtatg gcacggtaaa tgcttcgtag tgataacgtt ctttcaggtt
    27601 atcgcgcaat tgcaaaatat gcccgatggc gtcgtgcatt agttcgatag cgttaactcc
    27661 gcgtgctgga tcgctggagt gccccgactg gccctgaata cggatggcgt tagagatatg
    27721 acctttatgt gcgcgtaccg gttgtagtga cgtcggttcg ccaatgatgg cgcaatccgg
    27781 gcgcagggcg gtagtttcgg caaaataacg cgctccggcc atactggttt cttcatcagc
    27841 agtcgccaga atgtagagcg gttttttcag tttcgtgacg tcgacatcgc gtagcgtatc
    27901 aaggataaac gcaaaaaagc ctttcatgtc ggcggtgcct aagccgtaaa gcttgccgtc
    27961 atgctccgtc agtgtaaacg gatcgcgcgt ccagcgaccg tcatcaaatg gcaccgtatc
    28021 ggtatgcccc gccagcaaca agccgccagc cccctgtccg atacttgcca gcatattgaa
    28081 tttgttgcga gttcctggca caggctgcac ttccacattg aagcctaaat ctttaaacca
    28141 gtccgccagc agagtgatta aatctgcatt gctttgatcg agtgcctctt ccgtggcgct
    28201 tattgaaggt gtggcaatca gagcacggta aatctcgata aatggcggta atttgttttt
    28261 cattgttgac acacctctgg tcatgatagt atcaatattc atgcagtatt tatgaataaa
    28321 aatacactaa cgttgagcgt aataaaaccc accagccgta aggtgaatgt tttacgttta
    28381 acctggcaac cagacataag aaggtgaata gccccgatgt tgaatacgct gattgtgggt
    28441 gccagcggct acgctggcgc agagctagtg acctatgtaa atcgccatcc gcatatgaac
    28501 ataaccgctt tgactgtttc agcgcaaagc aatgatgcgg gaaagttaat ctccgatttg
    28561 catccgcagc taaaaggcat cgttgagctg ccgttgcagc cgatgtcgga tatcagcgag
    28621 tttagcccag gggtggacgt agtgtttctc gccaccgccc atgaagttag ccacgattta
    28681 gcgccgcagt ttcttgaagc gggctgcgtg gtgttcgacc tttccggtgc gtttcgtgtt
    28741 aacgacgcca ccttctatga aaaatattac ggctttaccc atcaataccc ggaactgctg
    28801 gaacaggcag cctacggtct ggcggagtgg tgcggtaata aattaaaaga agcgaatttg
    28861 attgcggtgc caggctgtta tccgacggcg gcacagctgg cgctgaaacc gttgattgat
    28921 gccgatcttc ttgacctcaa tcagtggccg gtgatcaacg ccaccagcgg cgtgagcggt
    28981 gcaggacgta aggcagcgat ttcaaatagt ttttgtgaag ttagcctgca accgtatggc
    29041 gtctttactc atcgccatca accagagatc gccacacacc tcggtgctga cgttatcttc
    29101 accccacatc tgggcaattt cccgcgcggc attctcgaaa ccattacctg ccgcctgaaa
    29161 tcgggtgtga cccaggcgca agtcgcgcaa gtgttacagc aggcgtatgc ccataaaccg
    29221 ctggtgcggc tgtatgacaa aggcgttccg gcgctgaaaa atgtcgttgg gctgccattt
    29281 tgcgatatcg ggtttgccgt tcagggcgag catctgatta ttgtggcgac cgaagacaac
    29341 ttactgaaag gcgcggcggc acaagcggta cagtgcgcca atattcgttt cggctatgcg
    29401 gaaacgcagt ctcttattta agggtgcaat gatgaatcca ttaattatca aactgggcgg
    29461 cgtactgctg gatagtgaag aggcgctgga acgtctgttt agcacactgg tgaattatcg
    29521 tgagtcacat cagcgtccgc tggtgattgt gcacggcggc ggttgcgtgg tggatgagct
    29581 gatgaaaggg ctgaatctgc cggtgaaaaa gaaaaacggc ctgcgggtga cgcctgctga
    29641 tcagatagac attatcaccg gagcactggc gggaacggca aataaaaccc tgttggcatg
    29701 ggcgaagaaa catcagattg cggccgtagg tttgtttctc ggtgacggcg acagcgtcaa
    29761 agtgacccag cttgatgaag agttaggtca tgttggactg gcgcagccag gttcgcctaa
    29821 gcttatcaac tccttgctgg agaacggtta tctgccggtg gtcagctcca ttggcgtaac
    29881 agacgaaggg caactgatga acgtcaatgc cgaccaggcg gcaacggcgc tggcggcaac
    29941 gctgggcgcg gatctgattt tgctctccga cgtcagcggc attctcgacg gcaaagggca
    30001 acgcattgcc gaaatgaccg ccgcgaaagc agaacaactg attgagcagg gcattattac
    30061 tgacggcatg atagtgaaag tgaacgcggc gctggatgcg gcccgcacgc tgggccgtcc
    30121 ggtagatatc gcctcctggc gtcatgcgga gcagcttccg gcactgttta acggtatgcc
    30181 gatgggtacg cggattttag cttaagtttt gttggccgga ggcgcagctt tccggcattg
    30241 aatttcaaaa taaggaaaca gagttatggc actttggggc gggcgtttta cccaggcagc
    30301 agatcaacgg ttcaaacaat tcaacgactc actgcgcttt gattaccgtc tggcggagca
    30361 ggatattgtt ggctctgtgg cctggtccaa agccctggtc acggtaggcg tgttaaccgc
    30421 agaagagcag gcgcaactgg aagaggcgct gaacgtgctg ctggaagatg ttcgcgccag
    30481 gccacaacaa atccttgaaa gcgacgccga agatatccat agctgggtgg aaggcaaact
    30541 gatcgacaaa gtgggccagt taggcaaaaa gctgcatacc gggcgtagtc gtaacgatca
    30601 ggtagcgact gacctgaaac tgtggtgcaa agataccgtt agcgagttac tgacggctaa
    30661 ccgacagctg caatcggcgc tggtggaaac cgcacaaaac aatcaggacg cggtcatgcc
    30721 aggttacact cacctgcaac gcgcccagcc ggtgacgttc gcgcactggt gcctggccta
    30781 tgttgagatg ctggcgcgtg atgaaagccg tttgcaggat gcgcttaagc gtctggatgt
    30841 cagcccatta ggctgtggcg cgctggcggg aacggcctat gaaatcgacc gtgaacagtt
    30901 agcaggctgg ctgggctttg cttcggcgac ccgtaacagt ctggacagcg tttctgaccg
    30961 tgaccatgtg ttggaactgc tgtcggctgc cgctatcggc atggtgcatc tgtcgcgttt
    31021 tgctgaagat ctgattttct ttaacaccgg cgaagcgggg tttgtggagc tttctgaccg
    31081 cgtgacttcc ggttcatcat taatgccgca gaagaaaaac ccggatgcgc tggagctgat
    31141 tcgcggtaaa tgcggtcggg tgcagggtgc gttaaccggc atgatgatga cgctgaaagg
    31201 tttgccgctg gcttacaaca aagatatgca ggaagacaaa gaaggtctgt tcgacgcgct
    31261 cgatacctgg ctggactgcc tgcatatggc ggcgctggtg ctggacggca ttcaggtgaa
    31321 acgtccacgt tgccaggaag cggctcagca gggttacgcc aacgccaccg aactggcgga
    31381 ttacctggtg gcgaaaggcg tacctttccg cgaggcgcac catatcgtgg gtgaagcggt
    31441 ggtggaagcc attcgtcagg gcaaaccgct ggaagatctg ccgctcgacg agttgcagaa
    31501 attcagcccg gtgattggcg aagatgtcta tccgattttg tcgctgcaat cgtgcctcga
    31561 caagcgtgcg gcaaaaggcg gcgtctcacc gcagcaggtg gcccaagcga ttgcttttgc
    31621 gcaggcgcgg ttggggtaag aacatttata tgtacaaatt tgagcctggc ttatcgccgg
    31681 gcttttttat ggcaaaaaaa agcggatcct ggagatccgc aaaagttcac gttggcttta
    31741 gttattcgag ttgagaaact ctcgaaacgg gcagtgactt caagggttaa aagaggtgcc
    31801 gctccgtttc tgtgagcaat tatcagtcag aatgcttgat agggataatc gttcattgct
    31861 attctaccta tcgccatgaa ctatcgtggc gatggaggat ggataatgaa tattcgtgat
    31921 cttgagtacc tggtggcatt ggctgaacac cgccattttc ggcgtgcggc agattcctgc
    31981 cacgttagcc agccgacgct tagcgggcaa attcgtaagc tggaagatga gctgggcgtg
    32041 atgttgctgg agcggaccag ccgtaaagtg ttgttcaccc aggcgggaat gctgctggtg
    32101 gatcaggcgc gtaccgtgct gcgtgaggtg aaagtcctta aagagatggc aagccagcag
    32161 ggcgagacga tgtccggacc gctgcatatt ggtttgattc ccacggttgg accgtacctg
    32221 ctaccgcata ttatcccgat gctgcaccag acctttccaa agctggaaat gtatctgcat
    32281 gaagcacaga cccaccagtt actggcgcaa ctggacagtg gcaaactcga ttgcgtgatc
    32341 ctcgcgctgg tgaaagagag cgaagcattc attgaagtgc cgttgtttga tgagccaatg
    32401 ttgctggcta tctatgaaga tcacccgtgg gcgaaccgcg aatgcgtacc gatggccgat
    32461 ctggcagggg aaaaactgct gatgctggaa gatggtcact gtttgcgcga tcaggcaatg
    32521 ggtttctgtt ttgaagccgg ggcggatgaa gatacacact tccgcgcgac cagcctggag
    32581 acgctgcgca acatggtggc ggcaggtagc gggatcactt tactgccagc gctggctgtg
    32641 ccgccggagc gcaaacgcga tggggttgtt tatctgccgt gcattaagcc ggaaccacgc
    32701 cgcactattg gcctggttta tcgtcctggc tcaccgctgc gcagccgcta tgagcagctg
    32761 gcagaggcca tccgagcaag aatggatggc tatttcgata aagtgttaaa acaggcggtt
    32821 taaaccgttt aacgcagcta cccgataggc ttccgccatg gtcgggtagt taaaggtggt
    32881 gttgacgaag tactcaatag tgttgccgcc acctttctgt tccataatcg cctgaccgat
    32941 atgaataatt tcggcagcgc gctcgccaaa gcagtgaata cccagaatct cttttgtttc
    33001 ccgatggaac aaaattttca gcgtgcccac gttcatgccg acgatttgtg cgcgtgccag
    33061 atgtttaaac tgggcgcggc ccacttcata tggcactttc atcgcggtca gctgctgttc
    33121 ggttttgccc acagagctga tttccgggat ggtgtaaatg ccggtaggga tatcttcaat
    33181 cagatgcgcg gtggcttcgc cttttaccag cgcctgcgcg gcaatgcgcc cctggtcata
    33241 ggccgctgat gccaggctcg gataaccaat cacgtcgccc accgcgtaaa cgtgcggctg
    33301 tgcggtctga tacatgctgt tgaccttcag ctgtccgcga ctgtcagttt ccagcccaat
    33361 gttctgtaac gccagcgaat cggtattacc ggtacgaccg ttggcataga gcaggcagtc
    33421 agctttcagt tttttacccg acttcagatg catgatcaca ccgtcgtcac agccttcgat
    33481 cttctcgtac tcttcgttgt gacgaatcac tacgccactg ttccagaagt gataggagag
    33541 agaatctgac atctcttgat cgagaaatgc cagcaggcga tcgcgggtgt tgatcagatc
    33601 cacttttaca tccataccgc ggaagatcga cgcatattca cagccgatca ctccagcacc
    33661 atagataagt acatggcgcg gttcgtggtg catgctaaga attgagtcgc tgtcgtaaat
    33721 gcgtggatgg gtgaaatcaa catctgttgg atgatatgga cgagagccgc aggcaataac
    33781 aaatttttca gcggttagtg tttcgacgct gccgtccggg caatccagcg ccaacgtatg
    33841 ctcgtcaaca aagcgagcgt tcccctgcaa tatttcacag tgattacgtt cgtaaaatcc
    33901 ctgacgcatg cgcgtttgtt gattaatcac gttatcggca tggttaagga tatcggcaaa
    33961 agaagagcgg agcagtcgcg aatggtcgct gtatagtggg ttttgattga attctataat
    34021 gcggctgacg gcgtgacgga gagctttcga cgggatggtg ccccagtggg tgcaaccgcc
    34081 gccaacattt tgataacgct cgataactgc gacgcgcaca ccttgcttaa ccaggcccat
    34141 tgcagcgcct tcgccgccgg ggccggaacc tattactatg gcatcgtaat cgtaggaatg
    34201 tggcatggta gggcttacct gttcttatac ataaaagcaa cagaatggta acattctatc
    34261 gcgggtaagc caattgatcc ccgtcattta tctggctata tcctgagcgg cctttgcttt
    34321 gtctgtttct tacttttgcc ctgacgtttt attggatttt tatcgacgat actctccgtt
    34381 taagcggcag gtttccgctg tacgtaaaag aaccggccag taaatatgtt ttattgcgtt
    34441 accgttcatt cacaatactg gagcaatcca gtatgttcat tctctggtat agtgccagca
    34501 gtacttttgg caaggattca gacatcgtga tgggcataag agcgcaacaa aaagaaaaaa
    34561 cccgccgttc gctggtggaa gccgcattta gccaattaag tgctgaacgc agcttcgcca
    34621 gcctgagttt gcgtgaagtg gcgcgtgaag cgggcattgc tcccacctct ttttatcggc
    34681 atttccgcga cgtagacgaa ctgggtctga ccatggttga tgagagcggt ttaatgctac
    34741 gccaactcat gcgccaggcg cgtcagcgta tcgccaaagg cgggagtgtg atccgcacct
    34801 cggtctccac atttatggag ttcatcggta ataatcctaa cgccttccgg ttattattgc
    34861 gggaacgctc cggcacctcc gctgcgtttc gtgccgccgt tgcgcgtgaa attcagcact
    34921 tcattgcgga acttgcggac tatctggaac tcgaaaacca tatgccgcgt gcgtttactg
    34981 aagcgcaagc cgaagcaatg gtgacaattg tcttcagtgc gggtgccgag gcgttggacg
    35041 tcggcgtcga acaacgtcgg caattagaag agcgactggt actgcaactg cgaatgattt
    35101 cgaaaggggc ttattactgg tatcgccgtg aacaagagaa aaccgcaatt attccgggaa
    35161 atgtgaagga cgagtaatga aacaagcaaa tcaagataga ggtacgctgc tgctggcgtt
    35221 agttgctggc ttatcgatta atggtacttt cgcagcgctg tttagctcca ttgtgccatt
    35281 ttctgtattc ccgattattt ccctggtgct gacggtttac tgcctgcatc aacgttatct
    35341 taatcgcact atgccggtag gcttgccggg tctggcagct gcctgtttta ttctcggcgt
    35401 actgctgtac agcacggtag ttcgtgcgga atatccggat atcggctcta acttcttccc
    35461 ggcagtactc tccgtcatta tggtgttctg gattggcgcg aagatgcgta accgtaagca
    35521 ggaagttgct gagtaatcgg tattatgccg gatgctggcg catccggcat gggttttact
    35581 tcgcggtcag taatacgccg cactccatat ggtgcgtgta ggggaactga tcaaacagag
    35641 ccagacgttc gaccttgtgc gtctggctta atgtttccag attcttgcat aacgtttccg
    35701 ggttacagga gatgtacaaa atacacggat acgcctgcac cattttctcg gtttcactgt
    35761 ccagaccgct gcgcggtggg tcgacaaaaa tggtttcgca ctgataactc tttaagtcga
    35821 tcccttgcag gcggttaaac tcgcgcacac cattcatcgc ctgagtaaat tcttctgccg
    35881 ccatacgaat aatttgtacg ttatcaatat ggttagctgc gatgttgtat tgcgcagcag
    35941 caaccgacgg cttagcgatt tcggtggcta atacccgatc aaaattgcgc gccagcgcta
    36001 atgaaaagtt accgttgccg cagtacagct ccagtaaatc gccttttgag cctttggtta
    36061 cgtccagcgc ccattccagc atctgaatat tcatcgccgc gttcggctgg gtaaagctgt
    36121 tttctacctg acggtagatc atctctttcc ctgcgaccgg cagacgctcg tcgatgtaat
    36181 cttgatccag ctcgattttg gttttcgttg cccgaccaat cagatgcaca ttcagattct
    36241 gcgcgcgcag tgcatcgcgc agggcctccg cttcctgacg ccactcatca tccagcttct
    36301 tatggtatag cagggaaacc accgcctgat tactcagcgt ggtgaggtaa tcaatctgga
    36361 acaacttgtg gcgcagaacg ggattattac gcacacccgc aatcatcgcc gtcatcaact
    36421 ggttgataag ttcactggcg gcggggaagc tatccacgcg gatacggctt ttggtttgtt
    36481 gatcgaaaat gatgtgatac aggtcatcgc catcgtgcca gatgcggaac tccgcacgca
    36541 tccggtaatg actgaccggc gagcgaaaca cttccggaac caggtcagaa aacggtgcca
    36601 tcatactttg caaacgtacc actttttcgg ctaactgcgc ttcatactgt tctgttggaa
    36661 ggtgttcggg ggtcatgatg tatcctgaaa aattaaagta cgcggcgatt gtagggattg
    36721 ctcatcagat gtccagatct tgatgaattc ctatttgtga gctacgtctg gacagtaact
    36781 tgttacaacc tgtagcatcc acttgtcggt cctgtgagtt aatagggaat ccagtgcgaa
    36841 tctggagctg acgcgcagcg gtaaggaaag gtgcgatgat tgcgttatgc ggacactgcc
    36901 atccggtggg aagtcatcat ctcttagtat cttagatacc cctccaagcc cgaagacctg
    36961 ccggccaacg tcgcatctgg ttctcatcat cgcgtaatat tgatgaaacc tgcggcatcc
    37021 ttcttctatt gtggatgctt tacaatgatt aaaaaagctt cgctgctgac ggcgtgttct
    37081 gtcacagcct tttccgcttg ggcacaggat accagcccgg atactctcgt cgttactgct
    37141 aaccgttttg aacagccgcg cagcactgtg cttgcaccaa ccaccgttgt gacccgtcag
    37201 gatatcgacc gctggcagtc gacctcggtc aatgatgtgc tgcgccgtct tccgggcgtc
    37261 gatatcaccc aaaacggcgg ttcaggtcag ctctcatcta tttttattcg cggtacaaat
    37321 gccagtcatg tgttggtgtt aattgatggc gtacgcctga atctggcggg ggtgagtggt
    37381 tctgccgacc ttagccagtt ccctattgcg cttgtccagc gtgttgaata tatccgtggg
    37441 ccgcgctccg ctgtttatgg ttccgatgca ataggcgggg tggtgaatat catcacgacg
    37501 cgcgatgaac ccggaacgga aatttcagca gggtggggaa gcaatagtta tcagaactat
    37561 gatgtctcta cgcagcaaca actgggggat aagacacggg taacgctgtt gggcgattat
    37621 gcccatactc atggttatga tgttgttgcc tatggtaata caggaacgca agcgcagaca
    37681 gataacgatg gttttttaag taaaacgctt tatggcgcgc tggagcataa ctttactgat
    37741 gcctggagcg gctttgtgcg cggctatggc tatgataacc gtaccaatta tgacgcgtat
    37801 tattctcccg gttcaccgtt gctcgatacc cgtaaactct atagccaaag ttgggacgcc
    37861 gggctgcgct ataacggcga actgattaaa tcacaactca ttaccagcta tagccatagc
    37921 aaagattaca actacgatcc ccattatggt cgttatgatt cgtcggcgac gctcgatgag
    37981 atgaagcaat acaccgtcca gtgggcaaac aatgtcatcg ttggtcacgg tagtattggt
    38041 gcgggtgtcg actggcagaa acagactacg acgccgggta caggttatgt tgaggatgga
    38101 tatgatcaac gtaataccgg catctatctg accgggctgc aacaagtcgg cgattttacc
    38161 tttgaaggcg cagcacgcag tgacgataac tcacagtttg gtcgtcatgg aacctggcaa
    38221 accagcgccg gttgggaatt catcgaaggt tatcgcttca ttgcttccta cgggacatct
    38281 tataaggcac caaatctggg gcaactgtat ggcttctacg gaaatccgaa tctggacccg
    38341 gagaaaagca aacagtggga aggcgcgttt gaaggcttaa ccgctggggt gaactggcgt
    38401 atttccggat atcgtaacga tgtcagtgac ttgatcgatt atgatgatca caccctgaaa
    38461 tattacaacg aagggaaagc gcggattaag ggcgtcgagg cgaccgccaa ttttgatacc
    38521 ggaccactga cgcatactgt gagttatgat tatgtcgatg cgcgcaatgc gattaccgac
    38581 acgccgttgt tacgccgtgc taaacagcag gtgaaatacc agctcgactg gcagttgtat
    38641 gacttcgact ggggtattac ttatcagtat ttaggcactc gctatgataa ggattactca
    38701 tcttatcctt atcaaaccgt taaaatgggc ggtgtgagct tgtgggatct tgcggttgcg
    38761 tatccggtca cctctcacct gacagttcgt ggtaaaatag ccaacctgtt cgacaaagat
    38821 tatgagacag tctatggcta ccaaactgca ggacgggaat acaccttgtc tggcagctac
    38881 accttctgaa ccacgtccca ccgtgctggt gtttgactcc ggcgtcggtg ggttgtcggt
    38941 ctatgacgag atccggcatc tcttaccgga tctccattac atttatgctt tcgataacgt
    39001 cgctttcccg tatggcgaaa aaagcgaagc gtttattgtt gagcgagtgg tggcaattgt
    39061 caccgcggtg caagaacgtt atccccttgc gctggctgtg gtcgcttgca acactgccag
    39121 taccgtttca cttcctgcat tacgcgaaaa gttcgacttc ccggttgttg gtgtcgtgcc
    39181 ggcgattaaa cctgctgcac gtctgacggc aaatggcatt gtcggattac tggcaacccg
    39241 cggaacagtt aaacgttctt atactcatga gctgatcgcg cgtttcgcta atgaatgcca
    39301 gatagaaatg ctgggctcgg cagagatggt tgagttggct gaagcgaagc tacatggcga
    39361 agatgtttct ctggatgcac taaaacgtat cctacgcccg tggttaagaa tgaaagagcc
    39421 gccagatacc gttgtattgg gttgcaccca tttccctcta ctacaagaag aactgttaca
    39481 agtgctgcca gagggaaccc ggctggtgga ttctggcgca gcgattgctc gccgaacggc
    39541 ctggttgtta gaacatgaag ccccggatgc aaaatctgcc gatgcgaata ttgccttttg
    39601 tatggcaatg acgccagaag ctgaacaatt attgcccgtt ttacagcgtt acggcttcga
    39661 aacgctcgaa aaactggcag ttttaggctg atttggttga atgttgcgcg gtcagaaaat
    39721 tattttaaat ttcctcttgt caggccggaa taactcccta taatgcgcca ccactgacac
    39781 ggaacaacgg caaacacgcc gccgggtcag cggggttctc ctgagaatct caacagagaa
    39841 agcaaaaata aatgcttgac tctgtggcgg gaaagcgtat tatgcacacc
//
LOCUS       DAZPLI010000023        39408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZPLI010000023 DAZPLI010000000
VERSION     DAZPLI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.023"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..266
                     /locus_tag="ACHUU3_001128"
     CDS             <1..266
                     /locus_tag="ACHUU3_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR2081260.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="ACHUU3_001129"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="ACHUU3_001129"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR2081261.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHUU3_001130"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHUU3_001130"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR2081262.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="ACHUU3_001131"
     CDS             complement(1570..3270)
                     /locus_tag="ACHUU3_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFR2081263.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="ACHUU3_001132"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="ACHUU3_001132"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR2081264.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4339..4797
                     /gene="decR"
                     /locus_tag="ACHUU3_001133"
                     /pseudo
     CDS             4339..4797
                     /gene="decR"
                     /locus_tag="ACHUU3_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
     gene            4827..6599
                     /locus_tag="ACHUU3_001134"
     CDS             4827..6599
                     /locus_tag="ACHUU3_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR2081265.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6592..8373
                     /locus_tag="ACHUU3_001135"
     CDS             6592..8373
                     /locus_tag="ACHUU3_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR2081266.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSVLRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8554..8892
                     /gene="glnK"
                     /locus_tag="ACHUU3_001136"
     CDS             8554..8892
                     /gene="glnK"
                     /locus_tag="ACHUU3_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR2081267.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8922..10208
                     /gene="amtB"
                     /locus_tag="ACHUU3_001137"
     CDS             8922..10208
                     /gene="amtB"
                     /locus_tag="ACHUU3_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR2081268.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10257..11117)
                     /gene="tesB"
                     /locus_tag="ACHUU3_001138"
     CDS             complement(10257..11117)
                     /gene="tesB"
                     /locus_tag="ACHUU3_001138"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR2081269.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11335..11907
                     /locus_tag="ACHUU3_001139"
     CDS             11335..11907
                     /locus_tag="ACHUU3_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR2081270.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11938..12249)
                     /locus_tag="ACHUU3_001140"
     CDS             complement(11938..12249)
                     /locus_tag="ACHUU3_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR2081271.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12412..12508
                     /gene="ffs"
                     /locus_tag="ACHUU3_001141"
     ncRNA           12412..12508
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHUU3_001141"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12628..12981
                     /locus_tag="ACHUU3_001142"
     CDS             12628..12981
                     /locus_tag="ACHUU3_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR2081272.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13023..14571)
                     /gene="pdeB"
                     /locus_tag="ACHUU3_001143"
                     /pseudo
     CDS             complement(13023..14571)
                     /gene="pdeB"
                     /locus_tag="ACHUU3_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14735..15205)
                     /locus_tag="ACHUU3_001144"
     CDS             complement(14735..15205)
                     /locus_tag="ACHUU3_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR2081273.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15321..15872)
                     /gene="maa"
                     /locus_tag="ACHUU3_001145"
     CDS             complement(15321..15872)
                     /gene="maa"
                     /locus_tag="ACHUU3_001145"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFR2081274.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16043..16261)
                     /locus_tag="ACHUU3_001146"
     CDS             complement(16043..16261)
                     /locus_tag="ACHUU3_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR2081275.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16287..16661)
                     /gene="tomB"
                     /locus_tag="ACHUU3_001147"
     CDS             complement(16287..16661)
                     /gene="tomB"
                     /locus_tag="ACHUU3_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR2081276.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(17207..20356)
                     /gene="acrB"
                     /locus_tag="ACHUU3_001148"
     CDS             complement(17207..20356)
                     /gene="acrB"
                     /locus_tag="ACHUU3_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR2081277.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(20379..21572)
                     /gene="acrA"
                     /locus_tag="ACHUU3_001149"
     CDS             complement(20379..21572)
                     /gene="acrA"
                     /locus_tag="ACHUU3_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR2081278.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            21714..22361
                     /gene="acrR"
                     /locus_tag="ACHUU3_001150"
     CDS             21714..22361
                     /gene="acrR"
                     /locus_tag="ACHUU3_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR2081279.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            22489..25851
                     /gene="mscK"
                     /locus_tag="ACHUU3_001151"
     CDS             22489..25851
                     /gene="mscK"
                     /locus_tag="ACHUU3_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR2081280.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            25912..26010
                     /locus_tag="ACHUU3_001152"
                     /pseudo
     CDS             25912..26010
                     /locus_tag="ACHUU3_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26063..26224)
                     /gene="rsmS"
                     /locus_tag="ACHUU3_001153"
     CDS             complement(26063..26224)
                     /gene="rsmS"
                     /locus_tag="ACHUU3_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR2081281.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(26238..26765)
                     /gene="priC"
                     /locus_tag="ACHUU3_001154"
     CDS             complement(26238..26765)
                     /gene="priC"
                     /locus_tag="ACHUU3_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR2081282.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            26835..27212
                     /locus_tag="ACHUU3_001155"
     CDS             26835..27212
                     /locus_tag="ACHUU3_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR2081283.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            27365..27916
                     /gene="apt"
                     /locus_tag="ACHUU3_001156"
     CDS             27365..27916
                     /gene="apt"
                     /locus_tag="ACHUU3_001156"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR2081284.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            28045..29970
                     /gene="dnaX"
                     /locus_tag="ACHUU3_001157"
     CDS             28045..29970
                     /gene="dnaX"
                     /locus_tag="ACHUU3_001157"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR2081285.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    29303..29367
                     /gene="dnaX"
                     /locus_tag="ACHUU3_001157"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            30023..30352
                     /locus_tag="ACHUU3_001158"
     CDS             30023..30352
                     /locus_tag="ACHUU3_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR2081286.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            30352..30957
                     /gene="recR"
                     /locus_tag="ACHUU3_001159"
     CDS             30352..30957
                     /gene="recR"
                     /locus_tag="ACHUU3_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR2081287.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            31067..32941
                     /gene="htpG"
                     /locus_tag="ACHUU3_001160"
     CDS             31067..32941
                     /gene="htpG"
                     /locus_tag="ACHUU3_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR2081288.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            33122..33766
                     /gene="adk"
                     /locus_tag="ACHUU3_001161"
     CDS             33122..33766
                     /gene="adk"
                     /locus_tag="ACHUU3_001161"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR2081289.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            33898..34860
                     /gene="hemH"
                     /locus_tag="ACHUU3_001162"
     CDS             33898..34860
                     /gene="hemH"
                     /locus_tag="ACHUU3_001162"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR2081290.1"
                     /translation="MRQTKIGILLANLGTPDTPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(34857..35816)
                     /gene="aes"
                     /locus_tag="ACHUU3_001163"
     CDS             complement(34857..35816)
                     /gene="aes"
                     /locus_tag="ACHUU3_001163"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFR2081291.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            35968..37272
                     /gene="gsk"
                     /locus_tag="ACHUU3_001164"
     CDS             35968..37272
                     /gene="gsk"
                     /locus_tag="ACHUU3_001164"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR2081292.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(37405..39081)
                     /gene="ybaL"
                     /locus_tag="ACHUU3_001165"
     CDS             complement(37405..39081)
                     /gene="ybaL"
                     /locus_tag="ACHUU3_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR2081293.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(39319..>39408)
                     /locus_tag="ACHUU3_001166"
     CDS             complement(39319..>39408)
                     /locus_tag="ACHUU3_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2081294.1"
                     /translation="SIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         9526 a        10049 c        10419 g         9414 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 gcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 attctccgtg gaagggctat gttagataaa attgaccgta agctgctggc cttactgcag
     4381 caggattgca ccctctcttt gtaggcactg gctgaagccg ttaatctgac aaccacccct
     4441 tgctggaagc gcctgaaacg gctggaggac gacggtatcc ttatcggcaa agtcgccctg
     4501 ctggatccgg aaaaaatagg cctcggcctg accgcctttg tgctgataaa aacgcaacat
     4561 cacagcagcg aatggtattg ccgctttgtc acggtggtta ccgaaatgcc agaagtgctg
     4621 gggttctggc gcatggctgg tgaatacgat tatctgatgc gcgtccaggt tgccgacatg
     4681 aaacgctacg acgagtttta taagcgtctg gtaaacagcg tgccggggct gtcggacgtc
     4741 acttccagct tcgcgatgga acagattaaa tacaccactt ctttacccat cgaataaata
     4801 tccagaatca ggtcaggaca caacgcgtgc gattatttgc tcaattaagc tggtatttcc
     4861 gtcgggaatg gcgtcgctat ctcggggctg tcgccttgct tgtcattatc gcgatgctgc
     4921 aactggttcc gccaaaagtg gttggtattg ttgtcgatgg cgtgacagaa caacacttta
     4981 ctaccgggct gatcctgatg tggatcgcca ccatggtgct gattgccgtt gtggtttatc
     5041 tcctgcgtta tgtctggcgc gtattgctgt ttggcgcgtc ttaccaactg gctgttgaac
     5101 tgcgtgaaga ttattaccgt cagctaagcc ggcagcaccc ggagttttac ctgcgtcatc
     5161 gcacgggtga tctcatggct cgtgcgacca atgacgtcga ttgcgtcgtg tttgccgccg
     5221 gagaaggggt gctgacgctg gtggattcac tggtaatggg ctgcgctgtg ttgattatga
     5281 tgtctacgca aattagctgg cagttgacct tatttgccct gttgccgatg ccagtgatgg
     5341 cgatcatgat taagcgcaac ggcgatgctt tgcatgaacg ctttaagctg gcacaggcgg
     5401 cgttttccag tcttaatgac cgcacccagg aaagcctcac cagtatccgc atgatcaaag
     5461 cctttggtct ggaagatcgc cagtcggcgt tatttgccgc ggatgccgaa gataccggca
     5521 aaaaaaacat gcgggtggcg cgtattgatg ctcgtttcga cccgaccatc tatatcgcga
     5581 ttggtatggc gaacttgctg gcgattggcg gcggtagttg gatggtggtg cagggcagtt
     5641 taacgctggg ccagctcacc agttttatga tgtatttagg tctgatgatt tggccaatgc
     5701 tggcgctggc atggatgttt aacattgtgg aacgtggtag tgctgcgtac agccgtattc
     5761 gtgcgatgct ggcggaagcg ccggtggtga acgatggtag cgaacccgtg ccggaagggc
     5821 gtggcgaact tgatgtaaat attcaccagt tcacgtatcc gcagactgac catcctgcgc
     5881 tggaaaacgt caatttcgcc ctgaaacccg gtcagatgct gggtatctgc gggccgactg
     5941 gttccggcaa aagtaccctg ttgtcgctca ttcagcgtca tttcgacgtt agcgaggggg
     6001 atattcgctt tcatgatatt cctctgacga agttacaact cgatagctgg cgtagccgcc
     6061 tggcggtggt cagccagacg ccattccttt tttctgacac cgtggcaaat aacatcgcgc
     6121 tgggttgccc gaatgccacc cagcaagaga ttgagcatgt cgcgcggtta gccagcgtac
     6181 atgacgatat tttgcgttta ccgcaaggtt acgatacaga ggtgggcgag cgcggcgtga
     6241 tgctttccgg cgggcaaaaa cagcgtatct ctattgctcg tgcgttatta gtcaacgcgg
     6301 aaatcctcat ccttgatgat gcgctttcgg cggtggacgg acgcacagag caccagatcc
     6361 tgcataacct gcgtcagtgg ggacagggaa gaacggtaat catcagtgcc catcgccttt
     6421 cggcactgac ggaagccagt gaaattattg tgatgcagca cggacatatc gcccagcgtg
     6481 gcaatcatga tgtgctggcg caacaaagcg gctggtatcg cgatatgtat cgctatcaac
     6541 aactggaggc ggcgctcgac gacgctccgg aaaatcacga ggaggccgtc gatgcgtagt
     6601 tttagccaac tgtggccgac tctcaagcgc ctgttagcgt acggttcacc gtggcgtaaa
     6661 ccgctgggga ttgcggtcct gatgatgtgg gttgcggcgg cggcagaagt cagtgggccg
     6721 ctgcttatca gctattttat cgacaatatg gtagcgaaaa ataacctgcc gttgaaagtg
     6781 gttgcagggc tggctgcggc gtatgttggg ctacaactgt ttgccgccgg gctacattac
     6841 gcgcagtcgc tgctgtttaa tcgggcggca gtaggcgtag tgcaacagtt gcgtaccgac
     6901 gtgatggatg ctgcgttacg ccagccatta agcgagtttg atacccaacc cgtcgggcag
     6961 gtgatttccc gcgtcactaa tgacactgaa gtgatccgcg atctctacgt taccgtagtg
     7021 gcaactgtcc tgcgcagtgc cgcactggtg ggcgcgatgc tggtggcgat gttcagcctc
     7081 gactggcgaa tggcgctggt ggcgataatg attttcccgg tagtgctggt ggtaatggtg
     7141 atatatcaac gttacagcac gccgattgtc cgtcgtgtgc gcgcctatct ggcggatatc
     7201 aacgacggct ttaacgaaat catcaatggc atgagcgtca tccagcagtt ccgtcagcag
     7261 gcgcgatttg gcgaacgtat gggtgaggcc agtcgttcac actatatggc gaggatgcaa
     7321 accctgcgcc tcgacggttt tctgctgcgt ccgctgctga gtctgttttc atcgctcatt
     7381 ctttgtggat tgttgatgct gtttggcttc tccgccagcg gcaccattga agtgggcgtg
     7441 ctgtatgcct ttatcagcta tcttgggcga cttaacgaac ccttaatcga actgaccacg
     7501 caacaggcga tgctgcaaca ggctgttgtt gctggtgagc gcgtgtttga actgatggac
     7561 ggaccgcgcc agcaatatgg caatgatgat tacccgttac agagtggcac catcgaagtc
     7621 gataacgtgt catttgctta tcgcgatgac aatctggtgc taaagaacat taatctctct
     7681 gtgccttcga gtaactttgt ggcgctggtc gggcataccg gcagtggtaa aagtaccctc
     7741 gccagtttat tgatgggcta ttacccgcta acggaaggtg agattcgcct tgatggtcgt
     7801 ccattaagtt cgctaagtca cagcgtgctg cgccagggcg tggcaatggt gcagcaagat
     7861 ccggtggtgc tggcggatac cttcctcgcc aacgtgacgc tggggcggga tatctccgaa
     7921 gaacgcgtct ggcaggcgct ggaaaccgtg caactggcgg agctggcgcg tagcatgagc
     7981 gacggtattt acacgccgct gggcgagcag gggaataatc tctcagttgg gcaaaagcaa
     8041 ctgctggcac tggcgcgcgt gctggtcgag acgccgcaaa tcctgatcct tgatgaggca
     8101 accgccagca ttgactccgg gactgaacag gcgattcaac atgctctggc ggcggtgcgt
     8161 gaacatacca cgctggtggt gattgctcac cgcttatcga ccattgttga tgccgacacc
     8221 attctggtgc ttcatcgtgg gcaagccgtg gagcagggca ctcaccagca actgctggct
     8281 gcccagggac gctactggca gatgtatcaa ctgcaacttg cgggcgaaga gctggcagcc
     8341 agcgtgcgtg aagaggaatc attgagcgcc tgaatagcgc aatatttcat cgttggtgca
     8401 aaaatgtaac gcactgtgca ctgtcatagt gcgttttcat tttcaaactt cttaacttcc
     8461 tgctctcttt ctcgtttttc atttctggca caccgcttgc aataccttct tcgtgtagca
     8521 gaaccattac cgaattctga ccggagggga tctatgaagc tggtgaccgt gataatcaaa
     8581 ccattcaagc tggaagacgt tcgtgaagcg ttatcttcca ttggtattca gggcctgacc
     8641 gtcaccgaag tgaaaggttt cgggcgtcag aaagggcatg ccgagctgta ccggggggcg
     8701 gaatacagcg tcaacttcct gccaaaagta aaaattgatg tggcgattgc tgatgaccaa
     8761 ctcgatgaag tgatcgatat cgtcagtaag gcggcttaca ccggaaaaat tggcgacggc
     8821 aaaatcttcg tcgctgaatt gcaacgcgtc attcgtattc gtaccggcga agccgacgaa
     8881 gcggcgctgt aatctctggc acacagcaac aggaacgaaa aatgaagata gcgacgataa
     8941 aaactgggct tgcttcactg gcgatgctgc cgggactggt aatggctgca cctgcggtgg
     9001 ccgataaagc cgacaatgcg tttatgatga tttgtactgc gctggtgctg tttatgacta
     9061 ttccggggat tgccctgttt tacggtgggt tgattcgcgg caaaaacgtg ctgtcgatgc
     9121 tgacgcaggt gacggtgacg tttgcactgg tctgtattct ctgggtggtt tacggttact
     9181 cgctggcgtt tggtgagggc aacaacttct tcggcaacat taactggttg atgctaaaaa
     9241 acatcgaact gacggcggtg atgggcagca tttatcagta tatccacgtg gcgtttcagg
     9301 gctcgtttgc ctgcattacc gtcggcttga tcgttggggc gctggcggaa cgaatccgct
     9361 tctcagctgt gttgattttc gtggtggtat ggctgacgct ctcttacatt ccgattgcgc
     9421 atatggtgtg gggcggtggt ttgctggctt ctcacggtgc gctggatttc gcgggtggca
     9481 cggtggtgca tattaacgcc gcaatcgccg gtctggtggg cgcgtatctg ataggcaaac
     9541 gcgtgggctt cggcaaagag gcgtttaaac cgcacaacct gccaatggtc ttcaccggta
     9601 ctgccattct ctatattggt tggtttggct ttaacgccgg gtcagcgggc acggcgaatg
     9661 aaatcgcggc actggcattt gtgaatactg tggtcgcaac ggcggcggca attcttggct
     9721 ggatcttcgg tgaatgggcg ctgcgtggta agccttctct gctgggagcg tgttctggcg
     9781 cgattgccgg tctggtcggc gtgacgccag cgtgtggcta cattggggtt ggcggcgcgt
     9841 tgattatcgg cgtggtagct ggtctggcgg gcttgtgggg cgttactatg ctcaaacgct
     9901 tgctgcgggt ggatgatccc tgcgatgtct tcggtgtgca cggcgtttgt ggcattgtcg
     9961 gctgtatcat gactgggatt tttgccgcca gttcgctggg tggcgtgggc ttcgctgaag
    10021 gtgtgacgat gggccatcag ttgctggtac agctggaaag catcgccatt acgatcgtct
    10081 ggtccggtgt ggtggcattt attggctaca aactggcgga tctgacggtt ggtctgcgtg
    10141 tgccggaaga acaggagcga gaagggctgg acgtcaacag ccacggcgag aatgcctata
    10201 acgcgtaaca agcactgcaa aaaacagccg gacggttttc acctccggct atttttttaa
    10261 ttgtgattac gcatcacccc ttcctgaacg gtcgaggcaa ccagcacgcc gtcctgagta
    10321 taaaactcac cgcgcacaaa gccgcgtgcg ctggacgccg aggtgctctc cacgctatac
    10381 agcagccatt cattcaaatt aaacgggcga tgaaaccaca tggaatggtc aatggtggca
    10441 atctgaatcc ccggttcgag aaaaccgatg ccgtgcggct gtagagctac cggcaggaag
    10501 ttaagatcag aagcgtaacc gagcagatac tgatgaacgc gcaggtcatc cggcacgcta
    10561 ccatttgcgc ggatccacac ctgacgatgt ggttctgcga cgtgaccttt cagtgggtta
    10621 tgaaactcca ccggacggac ttccagcgga cgatcgcaga tgaatttatc tttcagcact
    10681 ggcggcagca ggtgcgccag cgattgggcg atttgtgttt ccgaaggcag gccatcaggc
    10741 gctggcgcgg acggcattgt tttttgatgt tcgaaacccg cttcaggtgc ctggaaagag
    10801 gccgtcatat aaaaaatcgg tttgccgttt tgaatagcag caacccggcg ggcgctgaag
    10861 ctgttaccgt cacgcagcgt ttcgacatca taaataatcg gcttcttact atcgccaggg
    10921 cgaagaaagt agctgtgaaa cgaatgtacc agccgctctt cagggacggt ctcttttgca
    10981 gcatacaagg cctgacccac gacctggccg ccaaacacct ggcgtaaacc taaatcttca
    11041 ctctggccgc gaaagagtcc ttcctcaatt ttttccagat ttaacaatgt cagtaaattt
    11101 tttagcgcct gactcatata actctccagt aactaagctg ccgcagcaag ccaaagtgag
    11161 ttgagtataa cgcaaatttg ccactggtcc gatgggtgca atagtctgaa ttacgggcta
    11221 attacaggca gaaatgcgtg atgtgtgcca cacttgttga cgttactatt ttgttaacca
    11281 ctcttccggc gaggaaagtt agcccgctgg tgcattgata ataaggagaa gtgaatgaaa
    11341 ctcgtgcaca tggccagttg tttagcggtt gcgattgcgt tggcggcttg cgcagataaa
    11401 agcgcggata ttcagacgcc agcaccggcg gcaaatacgt ctatttcagc aacacaacaa
    11461 cctgctatcc agcaaccgaa tgtctccggt accgtttgga tccgtcagaa agtcgcactg
    11521 ccgcctgatg ctgtgctgac cgtgacactt tctgacgcgt cgttagccga tgcgccgtca
    11581 aaagtgctgg cgcagaaagc ggtgcgtacc gaaggtaaac agtcaccatt cagctttgtt
    11641 ctgcctttta acccggcaga tgttcagccg aacgcgcgta ttctgttgag tgcggcgatt
    11701 accgtgaatg acaaactggt atttattacc gataccgttc agccggtgat caacaagggc
    11761 ggaactaaag ccgacctgac attggtgccg atgcagcaaa ccgccgtgcc ggttcaggcc
    11821 agcggtggtg caacgactac cgtaccttcg acttcaccaa ctcaggtgaa tccgtcttcg
    11881 gcagttccag ctcctacgca atattaagcg acgttaaccc tctccgcccg gagagggtta
    11941 gtagttccag cgataacgct gcaagtcgat ttgcccgctt cccgatacca tcacaccttc
    12001 tgccagtaat gcctgtcgct gacgctgtaa atccggtccg gttagcgaaa ttgtgccgtg
    12061 gcgattaacc acccggtgcc agggtaaggt gctgccttca gggagacgct ttaacacacc
    12121 gcccacctgg cgcgcggcgc ggggcgatcc cgccagtttc gccacatcac cgtaagtggt
    12181 gacatagcct tcgggaatag cggcgacgat ttgccagacg cgttggggaa atgaatcttc
    12241 tttttccatc ttttcttcct gaggtaattt ttcagcataa tctggaaaaa cgcccgagtg
    12301 aagtcgcatt gcgcaagaaa ccagcatctg gcacgcgatg ggttgcaatt agccggggca
    12361 gcagtgataa tgcgcctgcg cgttggttct caacgctctc aatgggggct ctgttggttc
    12421 tcccgcaacg ctactctgtt taccaggtca ggtccggaag gaagcagcca aggcagatga
    12481 cgcgtgtgcc gggatgtagc tggcagggcc cccacccatt tctgcctccc accgtttcgt
    12541 caaaaaatcc caacatagct aaactttaac cacaactgac gtcgcaagaa ttgtctggct
    12601 gcgcagtacg cttcggaggt atgtctgatg aagtatgttg atggttttgt ggttgccgtt
    12661 cctgccgata aaaaggatgc ctatcgggaa atggccgcta aggctgcgcc attgtttaaa
    12721 gagtttggcg cgcttcgtat tgtcgaatgc tgggccagcg atgtaccgga tggcaaagtg
    12781 accgattttc gtatggcggt gaaagcggaa gagaatgaag aggttgtctt tagctggatt
    12841 gaataccctt caaaagaggt ccgcgacgct gctaatcaaa agatgatgtc ggacccacgg
    12901 atgaaagagt tcggcgagtc catgccgttt gacggcaagc gaatgatcta tggcggattc
    12961 gagtcaatca tcgacgaata ggtagcgtga cgggctgtgc ggcgtgccgt cagcccaaaa
    13021 attcacaaat gttgctcggc ccagcgtaag aaatcttctt tcggtaatgc cttgctgtag
    13081 agccagccct gaccgtagtg cacgccatgc tggcgtagcc actcttcttg tttactggtt
    13141 tcgattccct ccgctaccat tttcagtttc agtgtttttg ccatttcgat gatatgcggc
    13201 gtgacatttt tatattccag cgcatcaacg aaagatttat cgatcttcag aatgtcgaca
    13261 tccagatcct gtaaataact taaacttgaa tatcccgtac caaaatcatc aagataaatt
    13321 tcatggcccg cctcccggta gcgagaaatt atcggggcgc tggttttcgg atcggcaaac
    13381 tcgcgttcag tgagttcaag cgcgatctgt ctgggattaa cctgattgat tgatcatttc
    13441 acgcagcaat tgcgggattt tttccgaggt gagcacggtg gattcaagat tgatcgaaat
    13501 atgctgctgt ggatgctgac gcagccagtc gcccatatct tcaaaggcgc ttcttataat
    13561 cagtagcgtc aatggctcag aaaggcccgt ttgctgtgcc agcggaataa aactatctgg
    13621 tgacaaccaa ctaccgtctg tctgcggcca gcgcgccagt gcctcagcac cgacaatttt
    13681 gccattggct aaggagacaa tcggctgata gtgcacgcaa atatcacgat tttcgatagc
    13741 atcctgcagc cgatgatgcg gtgactggat acggcgcaaa atacgcagca caaacatcgc
    13801 tgccagcagg ccaatcacca acccgagcgg taaccaaata aagacttgcc gatgccagcc
    13861 tttctgcaac attttcgttg aagcccaggt gatgatcgaa atattcatct ccgggaaggg
    13921 caggatatcg taaatgattc cattattttc gatatgctca ccgggtgttt tttgtagcct
    13981 ggtaataatt ccctgagcaa tttcatcgct gctggttatg acaacgttat gggcattgcc
    14041 aataatggcg gcatcaattt gccatgagct atagggaatg acatcaataa aggaagcggg
    14101 gtcgatcatg acgacataat gtgccgttcc catggcgacc atgtaacgga taatgcctaa
    14161 atcgttatgc gatgttaacc agacacgata accatctttc gaaattttac ctggctcggg
    14221 gaaggtatcg ggcggacttt catgctccag agacgaacac tggggaacgt tgttatcgat
    14281 ataaaccact tcctgaatat agcgataact gtaagatacc cgacgcattt ccatgagatg
    14341 ggcttcgcta caggcagcgc cttgccatct ttccagctct tgcagcgcat ctttcccttg
    14401 tgtcgccacc ttattggccc gaatagcgac gcgggaggaa taggtatcca gctcttcaat
    14461 aaacgatgtt tctacctgct gatgggccag ccagatgctt aagccgacag gcagcaatac
    14521 tgaaagaatc agtactcccg aaataaggcc gaccagatgt cgtgttctca ccagcatatc
    14581 cttattaaca cagtgtaggg aaagtatatc tgattgtgga aaaaggctgg caggagataa
    14641 ggaggtagag ctggtatttc atgaagtgat gggtggaaac gatcattgtc atttgaccaa
    14701 gcgcagagct ttacgcctgg tatgtaaccc taacttaaga tgttgattcg gcgcgagcga
    14761 gggtaaaaat atcgtaaaaa gacagttcac ctttacggac gatcattttt tgcagttgtt
    14821 ccttatgaac atccgcgaca cgcggagagt tcaagatggc ttcaaccagc gcggcaggta
    14881 caaagcgcgt gttgtaccat tcgccgttat agcaaacgcg gaaatccagc acgtcgatat
    14941 cttcatagcg gtagcgtggg tagacatcga aaaagaagaa accattaagc aggataaata
    15001 atacaaccag tagccagaca gagcctgaca gcgtttcgga ctgcaacatc accgccaggg
    15061 tggcaaaaaa agcaacgtac ataccgataa acagccccgg atgtttacgg ataaaactga
    15121 tactaaagcg gggtttgttg tcgcgttttt cgcgggtatt cagagactca atcgtttcgg
    15181 tcagcaggcg ttgtatttcg gtcatcgttt gccttcgtgg tatctgcaac tttccaggga
    15241 acaccctatt ttaacgtggg aatagtagag gggaagtaac agatttacca caattttgca
    15301 taacagttgc gaaaaaccga ttacaatttt ttaattattc tggctggatt accgcccacg
    15361 acaacgttgt ccgggacatc ttttgtgaca actgcacctg aggctaccac gacgttatca
    15421 ccaatggtca caccagggtt aatgaccgcg cgtccgccaa tccagacgtt attaccgatg
    15481 gtgacgggtt tccccagttc agcaccgcta ttacgtgcta cagggtcgat gggatgtgtt
    15541 gccgtgtaga tatgaacgcc tggtgccaac atacagttat caccgatgcg aatagggcag
    15601 acatcaagca tcacgcaatc gaagttggcg aaaaaattat taccgagaaa aatgttatag
    15661 ccatagtcac agcgaaacgt tggctcaata taagcctctg tcacctgacc gaatagatca
    15721 gcgagaattt gctggcgtaa tgtgtgctct tccgccaggg aatgattgta tcggtgaata
    15781 agctgacgag cgcgcaggcg atcgcgagat aacgtctcat ctgccgagcg atacaactca
    15841 ccagcaatca tcttttcttt ttctgtgctc attactgacc tccggttgct gaaaatgcgg
    15901 caacacagta agacgaaaag tcagtgaaga gaacgttccc attacatctg tgtgataaag
    15961 atcacatagg gtatgtcttc atggtgaaaa gtataaaatt cttaataaac agccggttat
    16021 agctccgaaa gcgaattatt tattagcgaa taaatttcca tactgaggaa gggatcttgt
    16081 cgtatagttt attcatggtc aattcggcga ggcggtgatc tgcggctgag taaaataccg
    16141 ccagttcatt atctgataat tcgtatttat ttttctcgat aacacgctcc agcgtgtcaa
    16201 ttgtctggca acgacgtaaa cgcattaaat aatcggtttt cgttaaaggt ttttcggaca
    16261 tacttctacc catggttgta ataattctaa caagataaac tcgcaggatt ctctttcgtc
    16321 gcattgacaa aacaacggaa tagtcgatta cctgacttcc gccatttctg aagatcctgc
    16381 atattaatac cataactact gaacaacata aaggtgtcat ccagatattc gtcgatctgc
    16441 tcaatgagct tattgtcttc attatactta attttgtaat taagtgcgaa ggtcgcaata
    16501 tgctcaatca gttcattcaa ctggaggttg atcgccgagg ttgggtcgtt aacccagcca
    16561 tgattgcttt cttcaaggtt tgcaaggcag tcatgataca gggtttcaca gagaaactta
    16621 agctgtgcga tatcatgtct tttgggtgag tattcatcca taacgcgtcc ccttcttagc
    16681 ggttgaacta acggacacct ttcgggatgg aaaaaactta ctgacctgga cttgccctct
    16741 cgtttgttag cttaactata agccactctt tgcaggtttt catcgcatta ttacgaaaaa
    16801 ttacaattag gaaggaatcg ggacaggact cactttttca tactagcact aacccgcagc
    16861 aggtaaaagc agtctgctcg aactgttcat aacatttcag gattatcgcc agatgaaatt
    16921 ggtgacacta ataccaggat tgctctgaat atgacgtaat aaccgaggaa tgaataaaga
    16981 attaccgcaa ataattaaga atagcctcta aatgattatg gattattaaa tctattagcg
    17041 ctacttaata taaattaact aacaatcagt attttttatg aattttatcc gtggttaata
    17101 ctggttttcg tatgagatcc tgagttggtg gttcaattac tccttaatgt tcgtaggtta
    17161 tgcataaaaa aggccgcttg cgcggcctta gtgattacac gttgtatcaa tgatgatcga
    17221 cagtatggct gtgctcgata tcttcattct tgcggctaaa gcggcggcga accaccacaa
    17281 agaataccgg aacgaagaag attgccagta ccgttgcggt caccatcccg cccattacac
    17341 cggtacctac tgcgttctgc gcgccggaac cagcaccagt actgataacc agcggcataa
    17401 cgccgaggat aaacgccagc gaggtcatca ggatcggacg taaacgcatc cgcactgcat
    17461 caagcgtcgc ttcaatcaga cctttacctt ctttatccat caagtctttg gcgaattcga
    17521 cgataagtat cgcgttcttc gccgacaacc caatggttgt gagcaggcct acctggaagt
    17581 aaacgtcatt ggtcaggcca cggaaggtgg cagccagcaa cgcaccgata acccccagcg
    17641 gaacgaccag cataacggag aacggaatcg accagctctc gtacagcgcc gccagacaca
    17701 ggaatacgac aatcaacgaa atcgcgtaca gtgaaggtgc ctggttgccg gagagacgtt
    17761 cctgatagga catccccgtc cagtcatagc caacaccggt aggcagtttg ctcgccagtt
    17821 gttccatcag ctccattgct tcaccggtac ttttacccgg tgccgcctgg cctaagattt
    17881 ccatggatgg caggccgttg taacgttcca gacgcggcga accgtactcc caacgagaag
    17941 aggagaacgc cgagaatggc accatctgac catcagcagc acgaacatac cagtcgccga
    18001 tatcatccgg cagcatacgg tatttcgctt ctgacatgac gtaaactttc ttcacacgac
    18061 cgcggtcgat aaagtcgttc acatagctac cgccccatgc agcgcccaga gaggtgttaa
    18121 tgtcgttgat agaaacaccc agcgcctgcg ctttttcctg gtcgatatca atcttaaact
    18181 gcggggtatc ttccagaccg tttggacgta cgctggtcag catatcaggg tgcttcgctg
    18241 cttctgcaag cagctggtta cgcgcctgag tcagtttttc gtgaccaagg ccagcctggt
    18301 caatcagctc aaagtcaaag ccggttgcag tacccagttc cacgattgcg ggcaggttaa
    18361 aggcgaaaac catcgcatct ttgatttgcg agaaagcgcg tgttgcacgc atggtaatcg
    18421 cttcaacttt gttttcttcg cccggacgat cggcccagtc cttcaaggaa acgaacgcaa
    18481 taccggtatt ctgaccacgt cccgcaaagc cgaagccgtt aacggcgaac accgactcaa
    18541 cgttgttctt ttctttggtc agatagtaat gcgttacctc attgagcact ttctgcgtac
    18601 gttcctgcgt tgcacctgct ggcagctgaa ccatggtcat aaatacgccc tggtcctcat
    18661 ctggcaagaa ggagcttggc agacgcacga acagataggc catgccgacc acgatgatca
    18721 gatacagcac caggtaacgc cccgtactgc gcagaatacc gcctacgctg tcggtgtagt
    18781 ggtgcgtgct cttctcgaac atgcggttaa accagccgaa gaagcctttt ttaccttccc
    18841 cgtgatcgcc tttggcaatc ggtttcagca tggtggcaca aagagccgga gtcaggatca
    18901 acgctaccag taccgacagc gccattgctg aaacaatggt aatagagaac tgacgataga
    18961 tagcaccagt agaaccgcca aagaaggcca tcggtacgaa taccgccgac agtaccatcg
    19021 cgataccgac cagagcgccc tgaatctgcc ccatcgactt acgggtagct tcttttggcg
    19081 gcaaaccttc ttccgccata acacgctcca cgttttctac cacaacgata gcgtcatcca
    19141 ccaacaagcc gatggcgagc accatcccga acattgttag cgtgtttatc gagaagccaa
    19201 aggcggcaag gacggcaaag gtcccgagca ataccaccgg tacggcaatg gtcggaatca
    19261 acgtcgcgcg gaagttctgc aggaacagat acataaccag gaacacgagg atgatcgctt
    19321 cgaccagcgt tttaaccact tcgtgaatag agattttcac gaacggcgtg gtgtcgtacg
    19381 ggtaaacaat tttcagaccc gacgggaaga acggttccat cttcgccagt tcagcacgga
    19441 ttgccgcagc ggtatccagc gcgtttgcac cggtcgccag cttgatcccc agaccggaag
    19501 ccggttggcc gttaaactct gcgatgatgt cgtagttctc accacccagc tcaatcttcg
    19561 ccacatcacg cagcagtacg cgggaaccat cctgattcac tttcagcagg attttgccga
    19621 actcttcagt agaggtcaga cgcgtctgag caataataga ggcgttaagc tgttggcctt
    19681 tcaccggcgg cgtaccaccg agctgacccg ccgcaacctg ggcgttctgc gctttgatgg
    19741 cggtaatgac atcaaccggc gttagctgga atttgttcag ctcattcggg ttcatccaga
    19801 tacgcatcgc gtactgtgaa ccgaacaact gaacatcacc cacgcccgac gtacggctga
    19861 tggcatcttt catattcgcc gctacgtagt cagagatatc ctcctgcgtc atggtgccat
    19921 cggtgttgat aacgccgaca accatcagga agctgctgga tgatttctca acgctcaccc
    19981 cttgctgctg aacttcttgc ggcagcaacg gcatcgccag ctgcagtttg ttctgcacct
    20041 gaacctgcgc gatatccgca tcagtaccag actcaaaggt cagggtgatc tgcacggtac
    20101 ccgtggagtc actgttagag gacatgtaca tcaggttatc gataccgttc atattctgtt
    20161 cgataacctg tgtcaccgtg tcctgcactg ttttcgcatc agcgccgggg taggaggcgg
    20221 agatcgttac tgccggcggt gcaatcgtag gatattgcgc caccggcagt ttgaggatcg
    20281 ccagcccccc tgccaacatg atgataatgg cgatcaccca cgcaaaaatc gggcgatcga
    20341 taaagaaatt aggcatgtct taacggctcc tgtttaagtt aagacttgga ctgttcaggc
    20401 tgagcaccgc ttgcggcttg ctggttatta tcagcggtaa cttcttgtgc ttttacctgg
    20461 acaccaggac gcactttctg cagcccactt attactacgc gatcgcctgc tttcagacct
    20521 tctgtcacca gccacttatc gccaatagcc tggcttgcaa cgatcggacg ggtttccact
    20581 ttgtcatccg cgccaactac cagtacggtg gcatcaccac gcggcgtacg ggttacgccc
    20641 tgttgcggga ctaaaatagc gtttggatta agcccttctt ccagacgtgc gcgcacgaac
    20701 atacccggca gcagagtgtg atccgggttc gggaagatag cgcgtagggt gatagaccca
    20761 gtggtctgat caacggtaac gtcagagaat tccagcgtac cgtcctgcgg gaacttaatg
    20821 ccgtcactgg tgatcagcga cactttggct ttgccgttct cttgtttcag cgtgccattc
    20881 gccagttcct gtttcaggcg caggaagtcg ttgctggact gggtcacatc aaggtagatc
    20941 ggatcaagtt gctgcacggt tgccagcgca gtcgcctgac cgttctgtac caatgcgcct
    21001 tccgtcacgt tcgacttacc aatgcgaccg ctaatcggag aggtgacttt ggtgtaagcc
    21061 agattgatcc gcgcagtttc aacggcagct ttcgccgcag ttaccgcagc attcgcctgt
    21121 tgcgcatcag ccagagcctg atcgtactct tgcttactga tgtactgagt accgagcagt
    21181 ttctgataac gattcaccgt caattgcgcg atattggctg cagcctgggc tttcgccaga
    21241 tcacctttcg cactgtcgta tgtcgcctga taggtcgcag gatcaatctg atagagagag
    21301 acaccagctt cgatgtcgct accttctttg aaattacgct tcaggataat cccgctaact
    21361 tgaggacgaa cttctgcgat ccggtaggca ctggtgcgac ccggaagctc ggttgtgatc
    21421 tgcagaggtt cagttttgac tgttactacg ccaacggcgg gcatctgctg gccaccttgt
    21481 tgggcctgtt tgtcgtcaca tcctgttagg gctaagctgc ctgagagcat cagaacgacc
    21541 gccagaggcg taaaccctct gtttttgttc atatgtaaac ctcgagtgtc cgatttcaaa
    21601 ttggtcaatg gtcaaaagtt aataaaccca ttgctgcgtt tatattatcg tcgtgctatg
    21661 gtacatacat tcacaaatgt atgtaaatct aacgcctgta aattcacgaa catatggcac
    21721 gaaaaaccaa acaagaagcg caagaaacgc gccaacacat cctcgatgtg gctctacgtc
    21781 ttttctcaca gcagggggta tcatccacct cgctgggcga gattgcaaaa gcagctggcg
    21841 ttacgcgcgg tgcaatctac tggcatttta aagacaagtc ggatttgttc agtgagatct
    21901 gggaactgtc agaatccaat attggtgaac tagagcttga gtatcaggca aaattccctg
    21961 gcgatccact ctcagtatta agagagatat taattcatgt tcttgaatcc acggtgacag
    22021 aagaacgacg tcgattattg atggagatta tattccacaa atgcgaattt gtcggagaaa
    22081 tggctgttgt gcaacaggca caacgtaatc tctgtctgga aagttatgac cgtatagaac
    22141 aaacgttaaa acattgtatt gaagcgaaaa tgttgcctgc ggatttaatg acgcgtcggg
    22201 cagcaattat tatgcgcggc tatatttccg gcctgatgga aaactggctc tttgccccgc
    22261 aatcttttga tcttaaaaaa gaagcccgcg attacgtcgc catcttactg gagatgtatc
    22321 tcctgtgccc cacgcttcgt aatcctgcca ctaacgaata accctgaatc tgactccagg
    22381 atttttcctg gacattttcg tcgttgctat tctggttcac tgcgtcgtga tattcttgcg
    22441 gtttgacttt ttcaggtcgt tcttcaggtt cagaaacctt cattcatcat gactatgttc
    22501 cagtattaca aacgatcacg gcattttgtt ttttcagcat ttattgcttt tgtttttgtc
    22561 ttgttatgcc agaacacggc gtttgcgcgg gcgtcatcga atggtgatct gccgacaaaa
    22621 gcggacctgc aggcgcaact tgactcacta aataaacaaa aagatctttc tgctcaggac
    22681 aaactggtgc agcaggatct gacagataca ttagccaccc tcgataaaat cgatcgcata
    22741 aaagaagaga cagttcagct acggcaaaaa gtcgctgaag cgccggaaaa aatgcgccag
    22801 gcgaccgcgg cgttaacagc acttagcgat gtcgataacg acgaagaaac gcgcaaaatt
    22861 ctgagtacgc tttcgttgcg ccagctggaa actcgcgttg cccaggcgct ggacgatttg
    22921 caaaacgcac aaaacgatct ggcgtcttat aacagccagc tggtttcgtt acagacgcag
    22981 cccgaacgcg tgcaaaatgc gatgtataac gcttcgcagc agctgcaaca aattcgcagt
    23041 cgtctggatg ggactgatgt cggcgagact gccttacgtc ccagccagaa agtgttaatg
    23101 caggctcagc aggcgttgct gaatgcggag attgaccagc agcgtaaaag cctggaaggg
    23161 aacaccgtct tgcaggatac cttgcaaaag caacgtgatt acgtgacggc gaacagcgcc
    23221 cgtctggagc accagttgca gctgttgcaa gaagcggtaa acagcaagcg cctgacttta
    23281 actgaaaaaa cggcgcagga agccgtctcc ccggatgaag ccgcgcgtat tcaggctaat
    23341 ccgctggtga aacaggaact ggaaattaac cagcagttaa gtcagcgtct gattaccgcg
    23401 actgaaaacg gtaatcagtt gatgcagcaa aacattaaag tcaaaaactg gctggagcgg
    23461 gcgctgcaat cggaacgcaa tattaaagag cagattgccg tcctgaaggg cagcctgctg
    23521 ttgtctcgta tcctttacca gcaacaacaa acgctgccct cggcggatga actggaaaac
    23581 atgaccaacc gcatcgcgga tttgcgtctc gaacagtttg aagttaacca gcagcgtgat
    23641 gcactcttcc agagcgatgc gttcgtcaac aaactggaag aaggtcacac caacgaagtc
    23701 aacagcgaag ttcacgatgc gttattgcaa gtggttgata tgcgtcgcga attgctggat
    23761 caactcaaca aacagttggg taaccagctg atgatggcca ttaacctgca aatcaaccag
    23821 cagcagttaa tgagcgtatc gaaaaacctg aaatccatcc tgactcagca aatcttttgg
    23881 gtgaacagta accgtccaat ggactgggac tggatcaaag cgttcccgca aagcctgaaa
    23941 gatgaattta agtcgatgaa aatcacggtg aactgggaaa aagcctggcc tgccgttttt
    24001 atcgctttcc tcgccggttt gccgctgctg ttgattgccg ggctgatcca ctggcgtctg
    24061 ggctggctga aagcgtatca acagaaactg gcttccgctg tgggttccct gcgtaacgat
    24121 agccagctca acacaccaaa agcgatcctt atcgacctga tccgcgcgct gccggtgtgc
    24181 ctgattattc tcgcggttgg cctgattctg ttgaccatgc agctcaacat cagcgaactg
    24241 ctatggtcgt tcagtaaaaa actggcgatt ttctggctgg tgtttggcct gtgctggaag
    24301 gtactggaga aaaatggcgt tgccgtgcgt cacttcggca tgccggagca gcagaccagc
    24361 cactggcgtc ggcaaattgt ccgcatcagt ctcgcgttgc tgcctatcca tttctggtct
    24421 gtggtggcag aactttcccc gctgcatctg atggatgatg tgctggggca ggcgatgatt
    24481 ttcttcaacc tgctgctgat tgccttcctg gtatggccaa tgtgccgcga aagctggcgt
    24541 gataaagagt cgcacaccat gcgactggtc accattaccg tgctgtcgat tatcccgatt
    24601 gcattgatgg tgctgactgc cacgggttat ttctacacca cgctgcgcct ctccgggcgt
    24661 tggattgaaa ccgtttatct ggtgatcatc tggaacttgc tctaccagac ggtattgcgc
    24721 ggcttaagcg tagcggcgcg gcgtatcgcc tggcgtcgtg cgctggcgcg tcggcagaat
    24781 ctggtgaaag agggcgcaga aggtgccgaa ccgccggaag aacccaccat tgcactggaa
    24841 caggttaacc agcagacgct gcgtattacc atgttgctga tgtttgcgct gttcggtgtc
    24901 atgttctggg caatttggtc cgatttgatc accgtgttca gctatctcga cagcatcacg
    24961 ctctggcatt acaacggcac tgaagctggc gctgcggtgg tgaaaaacgt caccatgggc
    25021 agtctgttgt ttgcgattat cgcctcaatg gtggcctggg cgttgattcg caacctgcct
    25081 ggtttactgg aagtgctggt gctctcgcga ctgaatatgc gtcagggcgc gtcgtatgcc
    25141 attactacca tccttaacta catcattatt gccgttggtg cgatgacggt gttcggatcg
    25201 ctgggcgtct cttgggataa actccagtgg ctggccgcag cattatccgt aggtcttggt
    25261 tttggtttac aagaaatttt cggtaacttc gtctccggtt tgatcattct gttcgaacgt
    25321 ccggtgcgta ttggcgatac ggtaaccatt ggtagcttct cggggacggt aagtaagatc
    25381 cgtattcgtg cgacaacgat taccgatttc gatcgcaaag aagtgatcat cccgaacaaa
    25441 gcgtttgtta ccgagcgtct gatcaactgg tcgttgactg acactactac gcgtctggtg
    25501 atccgtctcg gcgtggccta tggctccgat ctggaaaaag tgcgtaaagt gttactgaag
    25561 gcggcgactg agcacccaag ggtgatgcac gaaccaatgc cggaagtctt ctttacggcg
    25621 tttggtgcca gcacgttgga tcatgagctg cgtctgtatg tgcgtgaact gcgtgaccgt
    25681 agtcgtactg tcgatgagct gaaccgtact atcgatcagc tgtgccgtga aaacgacatc
    25741 aacattgcct ttaaccagct tgaagtgcat ctgcacaacg agaagggcga tgaggtgacg
    25801 gaagtcaaac gcgactacaa aggcgatgac ccgacgccag cggtagggta ataaccaaag
    25861 ggggcgacat ttagttgccc cgagattact gacaaagtgc gcgttgttca tgccggatgc
    25921 ggcgtgaccg ccttatccgg cctacgaaac cgcaagaatt caatgtattg caggagcggt
    25981 gtaggcctga taagcgtagc gcatcaggca gttttgcgtt tgcccacaat cttaggggac
    26041 atttagcggc cccatttatt tctcactttt ccgcctcatc atcgcgcgtt aatttctttt
    26101 cataatcgcg ttttacaata tccagcgcgc gcagcacggt gcgggctggg atctgatttt
    26161 cttccagcag cacaatcaaa tcgacagcca gtttgacatc gtcaggggca ttttcaagtg
    26221 acatactctc tccattgcta gcgggttaag cgcgctagcc tgttttcgat tttttccagc
    26281 gcatggcggc agcgtgccag gcgcgcttca taggcttcca cttcacgatg cagcgtttgc
    26341 tgttccacga gatcggtcac ccgcgccagg cgggctctgc gttcggcaac catctcacgt
    26401 agccgccgct caaaatcctg atgctgaata cgtttacgct gccagcgggc aattttcggt
    26461 ggcgcactgt cccactcgcg caatgaccag gcggaggctt cacgcgcgat ggcttccagt
    26521 tgtgccgcca gatgttccgc cagccaggcc acttgcggca gctgttgctg ctcaaccgca
    26581 tgacaaagcg cagccagatt atcgcccgcc tcgtcgagac aagcctgtag tgttgtcgca
    26641 cgagtctgaa aaagatgcct gtcgaaacga gcacttagcg tggcgaactg tgacaccggg
    26701 gcacaacgct gacgcagcgt agcgagctgt ccttccagtt tttccagcag cagggcggtt
    26761 ttcacgataa gacctcaatg aaaatgataa ttgttatgct aaagtagcca ctctgtaagc
    26821 tgacaacact caatatgcaa cgaatcattt taatcatcat tggctggctg gcggtagtgc
    26881 tgggtacgct gggcgtggta ttaccggtat taccgacgac gccgtttatc ctgctggcgg
    26941 cctggtgctt tgcccgttct tccccgcgct ttcacgcctg gttgctgtac cgctcatggt
    27001 ttggcagcta tctacgtttc tggcagaaac atcatgcgat gccgcgcggc gtcaaaccgc
    27061 gggcgatttt gcttattttg ctcacgtttg ccatttctct gtggttcgtc cagatgccat
    27121 gggtgcgcat catgttgctg gtaattctcg cctgtttgct tttctatatg tggcgaattc
    27181 cggtgattga tgaaaagcaa gaaaagcact gaagcacaac aatcgcagtt gcaattattg
    27241 cgtacagcca gtacattctg gcgttttcga gcacaggcgc aggcggtcaa aggttaaaca
    27301 actgttactt ttgatacgtt taaaacgcgc cgtgagtacc accgtaacaa gcaggcatac
    27361 acttatgacc gcgactgcac agcagcttga gtatctcaaa aatagcatca aaagcattca
    27421 ggactaccca aaacccggca ttcttttccg cgatgtcacc agcttactgg aagacccgaa
    27481 agcttacgct ctcagcatcg acttgctggt tgagcgttac aaaaatgcgg gcattaccaa
    27541 agttgtcggc accgaagcgc gtggcttctt gtttggcgct ccggtagctc tgggtctggg
    27601 cgttggcttt gtaccggtcc gtaaaccggg caaactgccg cgtgaaacca tcagtgaaac
    27661 ttacgacctg gaatacggca ccgatcagct ggagatccac gttgatgcca tcaaaccggg
    27721 cgacaaagtt ctggtggtgg acgatctgct ggcaaccggc ggcactatcg aagcgaccgt
    27781 taaactgatc cgtcgtctgg gtggtgaagt ggctgacgct gcgttcatta tcaacctgtt
    27841 cgatctcggc ggcgaacagc gtctcgaaaa acagggcatt accagctaca gccttgtccc
    27901 gttcccgggc cattaattat cgccagtctt gtgctgccca cgctacggac agcacaagat
    27961 gtgcattcag cctcgccgtt ctgacggggc tgtgttagca ttaccccttc gtgaatccac
    28021 cttccagcgt ttcagagcct gccaatgagt tatcaggtct tagcccgaaa atggcgccca
    28081 caaacctttg ctgacgtcgt cggccaggaa catgtgctga ccgcactggc gaacggcttg
    28141 tcgttagggc gtattcatca tgcttatctt ttttccggca cccgtggcgt cggaaaaacc
    28201 tctatcgccc gactgctggc gaaggggcta aactgcgaaa ccggcattac cgcgacgccg
    28261 tgcggcgtgt gcgataactg tcgtgaaatc gagcaggggc gctttgtcga tctgattgaa
    28321 atcgacgccg cctcgcgcac caaagttgaa gatacccgcg acctgctgga taacgtccag
    28381 tacgctccgg cgcgtggtcg tttcaaagtt tatctgatcg acgaagtgca tatgctgtcg
    28441 cgccacagct ttaacgcact gttaaaaacc cttgaagagc cgccggagca cgttaagttt
    28501 ctgctggcga cgaccgatcc acagaaattg ccggtgacga ttttgtcgcg ctgtttgcaa
    28561 tttcatctca aggcgctgga tgtcgagcaa attcgccatc aacttgagca catcctcaac
    28621 gaagaacata tcgctcacga gccgcgggcg ttgcaattgc tggcgcgcgc cgctgaaggc
    28681 agcctgcgag atgccttaag tctgaccgac caggcgattg ccagcggtga cggccaggtt
    28741 tcaacccagg cagtcagtgc gatgctgggt acgcttgatg acgatcaggc gctgtcgctg
    28801 gttgaagcga tggtcgaggc caacggcgag cgcgtaatgg cgctgattaa tgaagccgct
    28861 gcccgtggta tcgagtggga agcgttgctg gtggaaatgc tcggcctgtt gcatcgtatt
    28921 gcgatggtac aactttcgcc tgctgcactt ggcaacgaca tggccgccat tgagctgcgg
    28981 atgcgtgaac tggcgcgcac cataccgccg acggatattc agctttacta tcagacgctg
    29041 ttgattggtc gcaaagaatt accgtatgcg ccggaccgcc gcatgggcgt tgagatgacg
    29101 ctgctgcgcg cgctggcgtt ccatccgcgt atgccgctgc ctgagccaga agtgccacgc
    29161 cagtcctttg cacccgtcgt gccaacggca gtaatgacgc caacccaggt gccgccgcaa
    29221 tcagcgccgc agcaggcacc gactgtaccg ctcccggaaa ccaccagcca ggtgctggcg
    29281 gcgcgccagc agttgcagtg cgtgcaggga gcaaccaaag caaaaaagag tgaatcggca
    29341 gccgctaccc gcgcgcggcc ggtgaataac gctgcgctgg aaagactggc ttcggtcacc
    29401 gatcgcgttc aggcgcgtcc ggtgccatcg gcgctggaaa aagcgtcagc caaaaaagaa
    29461 gcgtatcgct ggaaggcgac cactccggtg atgcagcaaa aagaagtggt cgccacgccg
    29521 aaggcgctga aaaaagcgct ggaacatgaa aaaacgccgg aactggcggc gaagctagcg
    29581 gcagaagcca ttgagcgcga cccgtgggcg gcacaggtga gccaactttc gctaccaaaa
    29641 ctggtcgaac aggtggcgtt aaatgcctgg aaagaggaga gcgacaacgc agtatgtctg
    29701 catttgcgct cctctcagcg gcatttgaac aaccgcggtg cacagcaaaa actggctgaa
    29761 gcgttgagca tgttaaaagg ttcaacggtt gaactgacta tcgttgaaga tgataatccc
    29821 gcggtgcgta cgccgctgga gtggcgtcag gcgatatacg aagaaaaact tgcgcaggcg
    29881 cgcgagtcca ttattgcgga taataatatt cagaccctgc gtcggttttt cgatgcggag
    29941 ctggatgaag aaagtatccg ccccatttga tcgtaagcac agcttacgtt cgtcatcctt
    30001 aacgtgattg agagagaaac ctatgtttgg taaaggcggt ctgggtaacc tgatgaagca
    30061 agcccagcag atgcaagaaa aaatgcagaa aatgcaggaa gagatcgcgc agctggaagt
    30121 caccggcgaa tctggcgcag gtctggtaaa agtgaccatc aacggtgcac acaactgccg
    30181 tcgcgtagag atcgacccga gcctgctgga agacgacaaa gagatgctgg aagacctggt
    30241 ggctgcagca ttcaacgacg cagcacgtcg tattgaagaa acgcagaaag aaaaaatggc
    30301 ctctgtatcc tccggaatgc agctgccgcc tggctttaag atgccgttct gatgcaaacc
    30361 agcccgctgt taacacagct tatggaagca ctgcgctgtc tgccgggcgt tggcccgaag
    30421 tcggcgcagc gtatggcgtt cacgctgctt cagcgcgatc gtagcggcgg gatgcgtttg
    30481 gcgcaggcgc tcacccgggc gatgtcggaa atcggccact gcgccgattg ccgcactttc
    30541 accgaacagg aagtctgtaa catctgttcg aatccgcgtc gtcaggaaaa cggtcaaatc
    30601 tgtgtggtgg agagtccggc ggacatctac gccattgagc agacggggca gttttctggt
    30661 cgttattttg tgttgatggg gcatctgtca ccgctggacg gcatcggtcc ggatgatatc
    30721 gggcttgatc gtctggaaca gcgtctggca gaggaaaaaa tcactgaagt gatcctcgcc
    30781 accaatccca cggttgaagg tgaagctacc gctaactaca ttgccgagct ttgcgcgcaa
    30841 tatgacgtgg aagccagccg aatcgctcat ggcgtaccgg ttggcggcga actggaaatg
    30901 gtcgatggca ccacgctgtc acactccctt gccgggcgtc ataagattcg tttttaagca
    30961 aacgagagca ggatcacctg ctctcgcttg aaattattct cccttgtccc catctctccc
    31021 tcatcctgtt tttaacctta aaatggcatt attgaggtag acctacatga aaggacaaga
    31081 aactcgtggt tttcagtcag aagtgaaaca gcttctgcac ctgatgatcc attctctcta
    31141 ttccaataaa gaaatcttcc tgcgtgagct tatctctaac gcctccgatg cggcggacaa
    31201 gctgcgtttc cgtgcgctat ctaacccgga cctgtacgaa ggtgatggcg aactgcgcgt
    31261 tcgtgtctct ttcgataaag acaagcgcac gctgaccatc tctgataacg gcgtggggat
    31321 gacccgcgac gaagtgattg accatctggg gactatcgct aaatccggta ccaaatcatt
    31381 cctcgaatcc ctgggttctg accaggcgaa agacagccag ctgatcggtc agtttggtgt
    31441 tggtttctac tctgcgttta tcgtggccga caaagtgacc gtgcgtactc gcgcggcagg
    31501 cgaaaaacca gaaaacggcg tcttctggga atcggctggc gaaggtgaat ataccgttgc
    31561 cgacatcacc aaagaagatc gtggtactga aatcaccctg catctgcgtg aaggcgaaga
    31621 cgagttcctc gatgactggc gcgtgcgttc catcatcagc aaatactccg accatatcgc
    31681 gctgccggta gagatcgaaa aacgcgaaga gaaagacggc gaaaccgtta tctcctggga
    31741 gaaaatcaac aaagcgcagg cgctgtggac tcgtaacaag tcggaaatca ccgatgaaga
    31801 gtacaaagag ttctacaaac acatcgccca cgactttaat gatccgctga cctggagcca
    31861 caaccgcgtt gaaggtaagc aggagtacac cagcctgctg tacattccgt cccaggctcc
    31921 gtgggatatg tggaaccgtg atcataaaca cggcctgaaa ctgtacgtcc agcgtgtgtt
    31981 catcatggac gacgcagaac agttcatgcc gaactatctg cgcttcgtgc gtggtctgat
    32041 tgactccagc gatctgccgc tgaacgtttc ccgtgaaatc ctccaggaca gcacggtaac
    32101 gcgtaacctg cgcaatgcgc tgaccaagcg tgtgctgcaa atgctggaaa aactggcgaa
    32161 agacgacgcg gaaaaatacc agaccttctg gcaacagttt ggcctggtac tgaaagaagg
    32221 tccggcggaa gatttcgcta accaggaagc gatcgccaaa ctgctgcgtt ttgcttctac
    32281 ccataccgat tcttctgcgc agaccgtatc tctggaagac tacgtttccc gcatgaaaga
    32341 agggcaggag aaaatctact acatcaccgc agacagctat gcggcagcga agagcagccc
    32401 gcacctggaa ctgctgcgta agaaaggcat cgaagttctg ctgctttccg accgcatcga
    32461 tgagtggatg atgaactatc tgactgagtt cgacggtaaa ccgttccagt cggtgtctaa
    32521 agttgacgag tcgctggaaa aactggctga cgaagttgat gagagcgcga aagaagcgga
    32581 gaaagcactg actccgttca tcgaccgtgt gaaagccctg ctcggcgagc gcgtgaaaga
    32641 tgtccgtctg actcaccgtc tgaccgatac gccagcgatc gtttcgaccg acgcggacga
    32701 aatgagcact cagatggcga aactgttcgc tgcggcgggc cagaaagtgc cagaagtgaa
    32761 atacatcttc gaactgaacc cggatcacgt actggtgaaa cgtgcggcag atactgaaga
    32821 tgaagcgaag ttcagcgagt gggtagaact gctgctggat caggcgctgc tggcagaacg
    32881 cggcacgctg gaagatccga acctgtttat tcgtcgtatg aaccagctgc tggtttcctg
    32941 atgtcatgcc ggatgacctt tgtgtcatcc ggcatttttc ttttcatcat ctgcactttc
    33001 cgcaaattat ctcgccatta accgtttcag cctcaggtgc ctttcttgag gcaatcgcct
    33061 gttggtggta tcgtttatcg ctttttcaaa aaaatcgaca cattttaagg ggattttcgc
    33121 aatgcgtatc attctgcttg gcgctccggg cgcggggaaa gggactcagg ctcagttcat
    33181 catggagaaa tatggtattc cgcaaatctc cactggcgat atgctgcgtg ctgcggtcaa
    33241 atctggctcc gagctgggta aacaagcaaa agacattatg gatgctggca aactggtcac
    33301 cgacgaactg gtgatcgcgc tggttaaaga gcgcattgct caggaagact gccgtaatgg
    33361 tttcctgttg gacggcttcc cgcgtaccat tccgcaggca gacgcgatga aagaagcggg
    33421 catcaatgtt gattacgttc tggaattcga cgtaccggac gaactgattg ttgatcgtat
    33481 cgtaggccgc cgcgttcatg cgccgtctgg tcgtgtttat cacgttaaat tcaatccgcc
    33541 gaaagtagaa ggcaaagacg acgttaccgg tgaagaactg actacccgta aagacgatca
    33601 ggaagaaacc gtgcgtaaac gtctggttga ataccatcag atgactgcac cgctgatcgg
    33661 ctactacttc aaagaagcgg aagcgggtaa caccaaatac gcgaaagttg acggcaccaa
    33721 gtcggttgct gaagttcgcg ctgatctgga aaaaatcctc ggctaatcaa gccgcagcag
    33781 atctgctttc gggcaggtct gctcatccac ctcaggcaat acctctcaca gcaattagtt
    33841 cttcttcctc acttttccgc tacaattatc aacaagttga atcgataaga ggcggtaatg
    33901 cgtcagacta aaatcggtat cctgctggca aacctgggta cgcccgatac ccccacacct
    33961 gaagcggtaa aacgctatct gaaacaattt ttaagcgaca gacgcgtggt tgatacctca
    34021 cggttgttat ggtggccatt gctgcgcggc gttattttgc cgctgcgctc gccgcgtgtg
    34081 gcgaagctgt acgcctcggt ctggatggaa ggtggctcgc cgctgatggt ttacagccgc
    34141 cagcaacagc aggcgctggc acaacgttta ccggagacgc ccgtagcgct gggaatgagc
    34201 tacggctcgc catcactgga aagcgccgtc gatgaactcc tggcagagca tgtagatcat
    34261 attgtggtgc tgccgcttta tccgcaattc tcctgttcta cggtcggtgc ggtatgggat
    34321 gaactggcac gcattctggc gcgcaaacgt agcattccgg ggatatcgtt tattcgtgat
    34381 tacgccgata accacgatta cattaatgca ctggcgaaca gcgtacgcgc ttcttttgcc
    34441 aaacatggcg aaccggatct gctgctgctc tcttatcatg gcattcccca gcgttatgca
    34501 gatgaaggcg atgattaccc gcaacgttgc cgcacaacga ctcgcgaact ggcttccgca
    34561 ctggggatgg caccggaaaa agtgatgatg acctttcagt cgcgctttgg ccgggaaccc
    34621 tggctgatgc cttataccga cgaaacgctg aaaatgctcg gagaaaaagg cgtaggtcat
    34681 atacaggtga tgtgcccggg ctttgctgcg gattgtctgg agacgctgga agagattgcc
    34741 gagcaaaacc gtgaggtctt cctcggtgcg ggcgggaaaa aatatgaata tattccagcg
    34801 cttaatgcca cgccggaaca tatcgaaatg atggctaatc ttgttgccgc gtatcgctaa
    34861 agctgagcgg taaagaactg agcgccgtcg cgaagagcct cgtcggcggt tttcatcatc
    34921 cgtgaataat gcaaaaaggc atgcagcgta cctgggtaga gtttgaactc acagggctgc
    34981 tgatgcgccg ctaacgtctg gtaaagcaga cggctgtcat ccagcagcgg atcgaactcc
    35041 gccccggcaa taaaacaggg cggaacttcg cgagtgagat cattattaaa cagacagtaa
    35101 tacggtgact cgcggtccgc gtcgttgctt aaatacgcct cttcatacat ctgcaaatcc
    35161 tgttgcgtta agccatccca gacaccgccc aacagacgac gagtcacgga atcccgtaat
    35221 ccgtaaagcc cataccacag caaaacaccc gcaattttac cgcaatcgat ctgtttatca
    35281 cgcaaccaca acgcactggc gagcgccagc atggcacctg cggaatcacc ggcaaagcca
    35341 atgcgggaca tattgatttg ataatcctcc gcctgctggt ggaaataaca acaagcagcc
    35401 acaatttcct ctatcgcttg cggaaaacgc gcttcaggtg aaagggtgta atcaatacca
    35461 atcaccgtac attggctgta gcttgccagc aggcgcatga tgcgatcgtg ggtatcgaga
    35521 ttgccgagaa taaaaccgcc tccatgcaaa taaaatagcg tcgccgggct atctggctgc
    35581 ggacaaaaga gacgtgtttc cacctgccca tattttgttg gaaccatgta agctctggtt
    35641 gccatttctg gagcgcccgc attccagaat cggcgctcaa gcgtgtaata ctgtcgttgc
    35701 tcagcaatcg ttcccgttgc gggccaggac ggtaaatccg gctgaagagt attcacaacg
    35761 gtcttcattt cagcagaaat aaggtccaga acaggtagtt tgttttccgg cttcatacaa
    35821 aaactccttt caaattacgt cattgtaagg aaccactgcc atgaaaatgc gatcccgcct
    35881 gctgatattg aaactggctg cgtctcgcgc gctcccgtca gattgtgtta acattcgccg
    35941 ctcagttaac cacccgtaaa aacaaccatg aaatttcccg gtaaacgtaa atccaaacat
    36001 tacttccctg taaatgcacg cgatccgctg cttcagcaat tccagccaga aaacgaaacc
    36061 agcgccgcct gggtcgtagg tattgaccag acgctggtcg atattgaagc gaaagtggat
    36121 gatgaattca ttgagcgtta tggattaagc gccgggcatt cactggtgat tgaggatgac
    36181 gtagccgaag cgctttatca ggagctaaaa cagaaaaacc tgattaccca tcagtttgcg
    36241 ggtggcacca ttggtaacac catgcacaac tactcggtgc tcgcggacga ccgttcggtg
    36301 ctgctgggcg tcatgtgcag caatattgaa attggcagtt atgcctatcg ttacctgtgt
    36361 aacacttcca gccgtaccga tcttaactat ctacaaggcg tggatggccc gattggtcgt
    36421 tgctttacgc tgattggcga gtccggggaa cgtacctttg ctatcagtcc aggccacatg
    36481 aaccagctgc gggctgaaag cattccggaa gatgtgattg ccggagcctc ggcactggtt
    36541 ctcacctctt atctggtgcg ttgcaaaccg ggtgaaccca tgccggaagc aaccatgaaa
    36601 gccattgagt acgcgaagaa atataacgta ccggtggtgc tgacgctggg caccaagttt
    36661 gtcattgccg agaatccgca gtggtggcag caattcctca aagatcacgt ctctatcctt
    36721 gcgatgaacg aagatgaagc cgaagcgttg accggagaaa gcgatccgtt gttggcatct
    36781 gacaaggcgc tggactgggt agatctggtg ctgtgcaccg ccgggccaat cggcttgtat
    36841 atggcgggct ttaccgaaga cgaagcgaaa cgtaaaaccc agcatccgct gctgccgggc
    36901 gctatagcgg aattcaacca gtatgagttt agccgcgcca tgcgccacaa ggattgccag
    36961 aatccgctgc gtgtctattc gcacattgcg ccgtacatgg gcgggccgga aaaaatcatg
    37021 aacactaatg gagcggggga tggcgcattg gcagcgttgc tgcatgacat taccgccaac
    37081 agctaccatc gtagcaacgt accaaactcc agcaaacata aattcacctg gttaacttat
    37141 tcatcgttag cgcaggtgtg taaatatgct aaccgtgtga gctatcaggt actgaaccag
    37201 cattcacctc gtttaacgcg cggcttgccg gagcgtgaag acagcctgga agagtcttac
    37261 tgggatcgtt aagttatcgt cggttcgtag gccagataag gcgttcacgc cgcatctggc
    37321 atttggctct cgatgcctga tgcgacgctg gcgcgtctta tcatgcctac atatttttca
    37381 tattttacat ccggcaacca ccgtttaccc cgtcaccact tcacccgccg gtggcgtttc
    37441 cagcagttcc agcatagtac gggcaatttc acgttcaccc atcactacct gattcgcgcc
    37501 acgttcggtg atatacgtca cttcatcgtc ataatgggcg cgggcgataa tctcaatatc
    37561 cgggtttttc gcgcgggcag acgccacaat ctcacccgct tcataaccgt tggggatcgt
    37621 caggatcagc cattttgcac attccagatg cgccagttgc ataatttctt cgttcgctgc
    37681 attgcccaat acagcgcgga ccccacgctc tcgcagctca tcaacacggg ttcgtgacgt
    37741 ctcaatcacc accagcggaa tatcagaggc gagcaatttc tcccccagca ggctgcctac
    37801 acgaccgtaa cccaccaata gcgcatggtt gcaaatatcc actgggatct gtttctcttc
    37861 ttcgattgcc tcttccagcg tctgctcttc cagcgtttcg gtcttcgcca gatatttctc
    37921 cagtagtgcg aacagtaccg ggttgagcat aatcgacagg atcgcccctg ccagtaccag
    37981 gttttgtccg gcctgcggca gtaaattcaa tgccattccc agtcccgcca ggataaacgc
    38041 gaactcacca atctgcgcca ggctggcggc gatggttaat gccgtacgtt gggagtgacc
    38101 aaacagacgg acaaggaaca gggccgcgac cgatttacca aacagaataa ttgccagcgt
    38161 cgccagcact gccagcggtt gctgaatcag aattaacgga tcaaacaaca tcccgacgga
    38221 gacaaaaaac agcaccgcaa acgcgtcgcg caatggcagc gtatcgtggg cggcacggtg
    38281 actcagttca gactcgttca gtaccatccc ggcaaagaac gcgccaagtg cgaaggagac
    38341 gtcaaacaac tccaccgcac caaaggcaat ccctaacgcc agcgccagca ccgacagggt
    38401 aaatagctcg cgagaaccgg ttgccgcgct gcgtgccata atccacggca ccagacggcg
    38461 acctaccagc atcataatgg cgataaatgc gatcactttg ccgatggtga tccccatatc
    38521 gactgcaaga gtggcaaagc ccacatcgcc ctgttccatc attcctgcca ctgcgggcag
    38581 caacaccagc gtcagaacca ttaccaggtc ttccacaatc aaccaaccga tggcgatttg
    38641 cccacgctga ctgtcaatta attgccgttc ttcaagtgcg cgcagtaaca ccacggtact
    38701 ggcggtggaa agacataaac cgaacacgat accggtcatt aacgaccagc ccagcacggc
    38761 agagagcgcc atacccagca gcgtcgccac ggctatctgg gcgatcgcac cgggaatggc
    38821 gatggccttt accgccatca aatccttcag cgaaaagtgc aaaccgacgc caaacatcaa
    38881 cagaatgacg cccagttcag ccagttccgg cgcaagcttg gtatcggcaa caaagcccgg
    38941 agtgaatggt cctgccagca cacccgctaa cagatatccc accagaggag aaatacgtag
    39001 tttattggcc agcatgccga ggataaaggc gagcacaagg ccgccaacaa tggtggtgat
    39061 aagcggggtg gcgtgatgca ttccgtctcc ttttcctggt ggttattgtc catttttggc
    39121 cgggaaaacc aaaattacag gtaatagttt atgacaattt cattgatgat gttcatgaat
    39181 aattattgaa ttttgcagaa aaatggaatt agctgcaaaa aaagcacgga tcggaaaaca
    39241 gaaggcggtt taacagagga aaggcttatg gcgactgtaa aacgatgcag ccaaagtttg
    39301 cctttggctg caatgaaatc agtctttatg ccggttatca ggcaggaata tggtcaacat
    39361 ccccaatagt ggcaggaaag cacagatttt atagactaac tcgatgct
//
LOCUS       DAZPLI010000024        38337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZPLI010000024 DAZPLI010000000
VERSION     DAZPLI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.024"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            59..1423
                     /locus_tag="ACHUU3_001167"
     CDS             59..1423
                     /locus_tag="ACHUU3_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2081295.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHUU3_001168"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHUU3_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR2081296.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="ACHUU3_001169"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="ACHUU3_001169"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR2081297.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3674
                     /gene="yajL"
                     /locus_tag="ACHUU3_001170"
     CDS             3084..3674
                     /gene="yajL"
                     /locus_tag="ACHUU3_001170"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR2081298.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHUU3_001171"
     CDS             complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHUU3_001171"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR2081299.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5382..5624
                     /gene="xseB"
                     /locus_tag="ACHUU3_001172"
     CDS             5382..5624
                     /gene="xseB"
                     /locus_tag="ACHUU3_001172"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR2081300.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLPPFTPDNE"
     gene            5624..6523
                     /gene="ispA"
                     /locus_tag="ACHUU3_001173"
     CDS             5624..6523
                     /gene="ispA"
                     /locus_tag="ACHUU3_001173"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR2081301.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6548..8410
                     /gene="dxs"
                     /locus_tag="ACHUU3_001174"
     CDS             6548..8410
                     /gene="dxs"
                     /locus_tag="ACHUU3_001174"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR2081302.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8465..9439
                     /gene="yajO"
                     /locus_tag="ACHUU3_001175"
     CDS             8465..9439
                     /gene="yajO"
                     /locus_tag="ACHUU3_001175"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR2081303.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHUU3_001176"
     CDS             complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHUU3_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR2081304.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHUU3_001177"
     CDS             complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHUU3_001177"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR2081305.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHUU3_001178"
     CDS             complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHUU3_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR2081306.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHUU3_001179"
     CDS             complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHUU3_001179"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR2081307.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHUU3_001180"
     CDS             complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHUU3_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR2081308.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHUU3_001181"
     CDS             complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHUU3_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR2081309.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13749..14288
                     /locus_tag="ACHUU3_001182"
     CDS             13749..14288
                     /locus_tag="ACHUU3_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR2081310.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14587..15471
                     /gene="tsx"
                     /locus_tag="ACHUU3_001183"
     CDS             14587..15471
                     /gene="tsx"
                     /locus_tag="ACHUU3_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR2081311.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHUU3_001184"
     CDS             complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHUU3_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR2081312.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHUU3_001185"
     CDS             complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHUU3_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR2081313.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHUU3_001186"
     CDS             complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHUU3_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR2081314.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHUU3_001187"
     CDS             complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHUU3_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR2081315.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHUU3_001188"
     CDS             complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHUU3_001188"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR2081316.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHUU3_001189"
     CDS             complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHUU3_001189"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR2081317.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21682..22263
                     /gene="acpH"
                     /locus_tag="ACHUU3_001190"
     CDS             21682..22263
                     /gene="acpH"
                     /locus_tag="ACHUU3_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFR2081318.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHUU3_001191"
     CDS             complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHUU3_001191"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR2081319.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHUU3_001192"
     CDS             complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHUU3_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR2081320.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHUU3_001193"
     CDS             complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHUU3_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR2081321.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHUU3_001194"
     CDS             complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHUU3_001194"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR2081322.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHUU3_001195"
     CDS             complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHUU3_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR2081323.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHUU3_001196"
     CDS             29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHUU3_001196"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR2081324.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30847..33990
                     /gene="sbcC"
                     /locus_tag="ACHUU3_001197"
     CDS             30847..33990
                     /gene="sbcC"
                     /locus_tag="ACHUU3_001197"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR2081325.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLTLENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34116..35300
                     /gene="araJ"
                     /locus_tag="ACHUU3_001198"
     CDS             34116..35300
                     /gene="araJ"
                     /locus_tag="ACHUU3_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR2081326.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35443..36351)
                     /gene="mak"
                     /locus_tag="ACHUU3_001199"
     CDS             complement(35443..36351)
                     /gene="mak"
                     /locus_tag="ACHUU3_001199"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR2081327.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36476..37387
                     /gene="rdgC"
                     /locus_tag="ACHUU3_001200"
     CDS             36476..37387
                     /gene="rdgC"
                     /locus_tag="ACHUU3_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR2081328.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37465..37920)
                     /locus_tag="ACHUU3_001201"
     CDS             complement(37465..37920)
                     /locus_tag="ACHUU3_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2081329.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYLMCSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37917..38252)
                     /locus_tag="ACHUU3_001202"
                     /pseudo
     CDS             complement(37917..38252)
                     /locus_tag="ACHUU3_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9467 a        10313 c         9733 g         8824 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     3301 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     3361 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     3421 tggtaccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     3481 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     3541 tgaccagcca ggggccgggt acggctattg attttggtct gaaaattatc gacctgctgg
     3601 ttgggcgcga gaaagcccat gaagtggcat cacaactggt gatggcggca gggatttata
     3661 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     3721 ccacaaatta tgggcgatac accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     3781 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     3841 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacgtcg ataccttcaa
     3901 ccttcagcgg cttatcttcc tgctcatcga cagaacggat atcgaggatc acgtcgttcg
     3961 ggccaaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     4021 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     4081 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     4141 cgccacaata ttctggcatc gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt
     4201 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcagagacgt
     4261 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     4321 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     4381 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     4441 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     4501 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     4561 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     4621 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccgcca ataccttcgt
     4681 agcggccttt aatcagcaga agacgatcgt cttccacttc cagatggaca gttacatccg
     4741 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     4801 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     4861 ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt
     4921 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     4981 cgtcgcgaat agccagacgc tgtttttcat cttttgcgcg aacttcgatg ttatcccagt
     5041 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     5101 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     5161 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     5221 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     5281 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     5341 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     5401 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     5461 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     5521 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     5581 atgaagacgc ctctctaccc ccttttacac cggacaatga gtaatggact ttccgcagca
     5641 acttgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     5701 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     5761 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     5821 ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
     5881 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     5941 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattttaag
     6001 cgatgccgat atgccggaag tgtcggaccg cgacagaatt tcgatgattt cagaactggc
     6061 aagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag atgcggaagg
     6121 caaacacgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
     6181 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     6241 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     6301 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     6361 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     6421 gatcgacgat gcccgccagt cgctgaaaca actggctgaa caatcactcg atacctcggc
     6481 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     6541 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     6601 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     6661 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     6721 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     6781 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     6841 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     6901 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttactgccga
     6961 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
     7021 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
     7081 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
     7141 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
     7201 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
     7261 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
     7321 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
     7381 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
     7441 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
     7501 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
     7561 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
     7621 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
     7681 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
     7741 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
     7801 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
     7861 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
     7921 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
     7981 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
     8041 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
     8101 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
     8161 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
     8221 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctaatgg cccatcgtaa
     8281 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
     8341 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
     8401 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
     8461 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8521 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactgc cggaagagag
     8581 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8641 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8701 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8761 attatcccgt gcacaaattt tgcgctctat cgacgacagc ctgcgccgtc tcggcatgga
     8821 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     8881 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     8941 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9001 tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
     9061 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9121 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9181 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
     9241 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta aaccgggcat
     9301 tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctgc tgaacgcggt
     9361 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9421 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9481 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9541 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9601 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9661 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9721 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9781 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
     9841 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
     9901 aggaacgatt gggcttaatc cacttccgaa tccgacagca agcagatgcc acggattact
     9961 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10021 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10081 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    10141 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10201 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcta
    10261 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10321 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10381 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10441 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10501 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10561 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10621 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10681 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10741 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    10801 ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    10861 tattgagaag tgcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    10921 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    10981 caggtaaaaa agaaaaggcc gacgatcatg gaacggtctt ccgtgaatct accggcctgg
    11041 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11101 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11161 tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    11221 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11281 gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11341 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11401 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11461 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11521 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11581 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11641 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11701 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11761 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    11821 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    11881 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    11941 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12001 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12061 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12121 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12181 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12241 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    12301 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12361 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12421 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12481 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12541 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    12601 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12661 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12721 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12781 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    12841 tcctgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    12901 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    12961 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    13021 taaccttcac cgacaatttc gccatctttg acaatgacgc acccgacatt cgggttgggg
    13081 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    13141 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13201 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13261 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    13321 gatttaatat gattgattgc catttcaacg tcatcggaac tcaccggacg tttttccagc
    13381 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13441 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13501 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13561 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13621 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13681 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13741 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13801 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    13861 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    13921 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    13981 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    14041 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    14101 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14161 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    14221 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    14281 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatctttcc ccgtcatttt
    14341 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    14401 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14461 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14521 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14581 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14641 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14701 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14761 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    14821 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    14881 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    14941 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15001 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15061 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatga cgcagcgaac
    15121 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15181 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15241 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15301 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15361 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15421 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    15481 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    15541 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    15601 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15661 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15721 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15781 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    15841 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg
    15901 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    15961 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    16021 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16081 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16141 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16201 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    16261 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    16321 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16381 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    16441 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16501 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16561 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    16621 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16681 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16741 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16801 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    16861 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    16921 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    16981 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17041 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17101 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17161 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17221 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17281 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17341 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17401 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    17461 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    17521 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17581 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    17641 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17701 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17761 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    17821 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    17881 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    17941 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18001 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18061 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    18121 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc aggcagttca
    18181 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18241 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18301 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    18361 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    18421 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    18481 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18541 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18601 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18661 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    18721 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18781 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    18841 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    18901 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    18961 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19021 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19081 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19141 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19201 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19261 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19321 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19381 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19441 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19501 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19561 ctcaggatca agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19621 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19681 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19741 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19801 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    19861 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    19921 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    19981 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20041 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20101 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20161 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20221 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20281 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20341 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20401 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20461 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccacggaatt
    20521 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20581 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20641 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20701 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    20761 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    20821 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    20881 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    20941 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21001 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21061 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21121 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21181 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21241 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21301 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21361 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21421 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21481 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21541 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21601 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21661 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21721 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    21781 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    21841 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    21901 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    21961 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22021 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22081 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22141 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22201 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22261 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22321 tcagttgccc atgcctttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22381 gtagcacgac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22441 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22501 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22561 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22621 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22681 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22741 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22801 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    22861 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    22921 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    22981 cttccggctg ggtttctttc gccgcttcgg tgctcccagc aacgtgctgc atattattgc
    23041 gcgccccacc cgcctcccct agcatatgca ccacatccag ccgccagccg tccatactcc
    23101 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    23161 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    23221 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    23281 cccccgtgcc acgattgtgc ctgtcaaacc aggcatggga atcgccactg tggttaaaca
    23341 cgccatccag ccccagccgc attcccagct gctgcgtatt gtggcgtaag cgcagcaacg
    23401 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    23461 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    23521 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    23581 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    23641 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    23701 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    23761 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    23821 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    23881 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgatgctta tgcatcgata
    23941 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    24001 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    24061 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    24121 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    24181 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagga
    24241 ttactggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc caatcaacag
    24301 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    24361 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    24421 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cagcgacgga aggcaatttg
    24481 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    24541 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    24601 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    24661 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaaga cgtcactgtt
    24721 aattgccgat agcgaagcgg tcagcacaac aaagttaaga atgctggcgg caaaggtaat
    24781 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    24841 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    24901 ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    24961 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca ccatcccaag
    25021 ccagccgtta ctgaagaagc cgccgttgct ccacagatta tgaataccgg tcggttgccc
    25081 gccgttgcca atcccccaga tgatgatgcc gaaaccagcg acaatcatga tgatgatggt
    25141 ggcgacttta aagaacgaga accagaattc cagctccccg aagaccttca cgctcatcag
    25201 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    25261 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    25321 ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
    25381 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    25441 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    25501 tttgatggcg tctgccgaac cgtaaaacag cccggtacca attgctgaac ccagtgccat
    25561 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
    25621 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    25681 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    25741 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    25801 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    25861 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    25921 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    25981 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
    26041 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    26101 tgcacaagca caggtcaggc caactgccgt taccaagcag gcgatgaaga ttaacgccgc
    26161 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    26221 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
    26281 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    26341 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    26401 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
    26461 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac
    26521 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    26581 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    26641 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
    26701 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    26761 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    26821 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    26881 agtccagacg tgttcgccag cctgcaagcc gaccattgga gggaaaataa tgttacctgc
    26941 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    27001 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    27061 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    27121 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    27181 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    27241 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    27301 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    27361 aagaaaataa gccagcatcg actggcttat ggcaataaaa gatgacaaag gcggattaat
    27421 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    27481 ctgtactctc aatattcagg cgactttcgt gatgattcac agcatgtttc acgatcgcta
    27541 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    27601 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    27661 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
    27721 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    27781 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    27841 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    27901 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    27961 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    28021 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    28081 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    28141 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    28201 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    28261 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    28321 gaatttgttg cgccagaccg ttacaccaga aaataccgct ctcttccgtg gtcagcacca
    28381 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    28441 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    28501 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    28561 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    28621 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    28681 ccagcctttt ccacgacagc cgttccagca cgtaagatac tcccgttaag aaatcataag
    28741 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    28801 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    28861 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    28921 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    28981 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    29041 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
    29101 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
    29161 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    29221 ccactggaat atcccgggtc atcgactcgc gcttaaggtg tttgatgaac tggataccgg
    29281 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    29341 tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    29401 agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    29461 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    29521 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    29581 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgccaggg
    29641 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    29701 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag cacaaaccca
    29761 tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc cgcccagtta
    29821 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    29881 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    29941 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    30001 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc gtgacattat
    30061 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    30121 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    30181 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ccgtgcgtga
    30241 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    30301 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    30361 cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    30421 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
    30481 catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    30541 tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg agtatctgca
    30601 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    30661 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    30721 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
    30781 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
    30841 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    30901 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
    30961 acaggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact
    31021 ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgccgaa
    31081 tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt accgtgcatt ctggagccag
    31141 aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc cacgcgtaga gctggcgcgc
    31201 tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg
    31261 ttaaccgggc tggattacgg gcgcttcact cgttcgatgc tgctttcgca ggggcaattt
    31321 gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc
    31381 actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcaca
    31441 gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg
    31501 caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg
    31561 cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa
    31621 gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct
    31681 caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc
    31741 atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact
    31801 cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca
    31861 gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc
    31921 cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat
    31981 cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg
    32041 cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctagcgccct ggcgcaacat
    32101 tccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa
    32161 caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga acagacgcaa
    32221 cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc
    32281 gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc tcaacgtgca
    32341 cagttacagg cgggtcaacc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag
    32401 gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactgacgct ggaaaacgaa
    32461 gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag
    32521 cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact
    32581 caacaatggc aagccgtcac gaccagcctc aatatcacct tgcagccaca ggacgatatt
    32641 caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga
    32701 catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa
    32761 attgaacaac gccagcagca gctcttaacg gcattgacgg gttatgcact gacattgcca
    32821 caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag
    32881 caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac gccgattctg
    32941 gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac
    33001 tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa
    33061 caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga caccgcgcta
    33121 caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca
    33181 ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg
    33241 ctggtcacac agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg
    33301 gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg
    33361 cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac
    33421 cgtcagcaac aacaaacctt actgcagcaa attgctcaaa tgacgcagca ggttgaggac
    33481 tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc
    33541 caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac
    33601 gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc
    33661 tggcaggcag atgcagtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt
    33721 agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg
    33781 ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat
    33841 gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgttgaagcg
    33901 atgaaagagc gtattcctgt gcagatcaaa gtgaagaaga tcaacggcct gggctacagc
    33961 aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg
    34021 cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg
    34081 tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg
    34141 ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg ctcacggagc
    34201 tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac
    34261 tgggggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac
    34321 atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt
    34381 cgtcttacct gatgcttgcc attggtcgcc tggtttccgg ctttccgcat ggcgcatttt
    34441 ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg
    34501 tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt
    34561 atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt tttaatattg
    34621 cggtgatggc atcggtctat ttttgggtgc cagatattcg cgacgaggcg aaaggaaatc
    34681 tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc gccgccacga
    34741 tgtttggcaa cgcaggtgtc tttgcctggt tcagctacgt aaagccatac atgatgttta
    34801 tttccgtttt ttcggaaacg gcgatgacct ttattatgat gttagttggg ctagggatgg
    34861 tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg cgcattgcag
    34921 cagtgactga ctttatcatt gtactggcac tgctgatgct ctttttctgc ggcggcatga
    34981 aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc ctttcagcac
    35041 ctctgcaaat attgttactg caaaacgcca aaggcggaga gttattaggt gccgcaggtg
    35101 ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt atgatgctga
    35161 cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg tttgctgcga
    35221 tgtcatcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat agtccggtgc
    35281 tggcgaaacc actggggtag attactgtca cggtgcgcgt tgcttattgc cggatgcggc
    35341 gtgaacgcct tatccgccct acgcggttct ggcacatttt gtaggcctga taagacgcgg
    35401 caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt ttttactctt gtggccataa
    35461 ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttacgca ccggcgtttc
    35521 acattcgccg ccgaagacaa attgtttaat caactgccca accgtttgat ataaacggtc
    35581 tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg
    35641 tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc
    35701 gctttcttca accaggcgga taatttcact gcctttcagc gcatgtccgc tcaaacgacg
    35761 ataatccgtc gcgaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca
    35821 ataacaaggg acttcctcgc gataacgcag ctcgtcttca tccatccacg gtagcggatt
    35881 gtgtccccac tcgcctgccg tgccattgcc gccgatatgc gcccgcccat ttaatgccac
    35941 gcccgcgccg catcccgtgc cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc
    36001 gccatctact gcttctgaaa ccgccagaca gttggcatca tttgccagcc gcacttcccg
    36061 ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattggc
    36121 attcttcacc acaccggtgt agggcgaaat cgaacctgga atgcccatac ctaccgttcc
    36181 gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg
    36241 ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc
    36301 cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca ctgtactctc
    36361 cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa tgcaaggccg
    36421 ccgacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct
    36481 gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc gtgcagaaga
    36541 gatggaaaaa cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa
    36601 gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg
    36661 tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca
    36721 ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac
    36781 cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ctgccgcgtg ctttcagccg
    36841 ttttagccag acaatgatgt ggatcgacac ggttaacggt ttgattatgg tggactgcgc
    36901 cagtgccaaa aaagcggaag atacgctggc attactgcgt aaaagcctgg ggtcgttacc
    36961 ggttgtacca ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat gggttcgctc
    37021 cggtagtgcg gcacagggct tccagctgct tgatgaagcc gagctgaaat cgttgctgga
    37081 agatggcggc gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaatca
    37141 cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt
    37201 tgtgatgtgc gacgatggtt cgctcaagcg tcttaagttc tgcgacgagc tgcgcgatca
    37261 aaacgaagat atcgaccgtg aagatttcgc cctgcgtttt gatgccgatt tcatcctgat
    37321 gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca
    37381 acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg
    37441 tacgaagcca caccaggcat ataattattc gctacggcga gcaataattt ttagcgcagc
    37501 aatattatgc gttttacgcc gtaactgatc taatcgcctc atccctattc gcctgtcagc
    37561 agcacgcaga atattcacta acccccattt atcgctggta aattgttttg ttaacagctc
    37621 cgcttttggg gtatcaatat attcacgcaa taaatcagaa attgaacaaa tatccgtcag
    37681 gtaaggatac cagcaagttt caccgtgaaa attacgcact ccagcgttac ctgcaataaa
    37741 gtctttcggg taatcccaga tcacgttttt atccaacgtg atccaataac gcggtaaatc
    37801 ggtacttcca ccattttggc tgcacatgag ataacgggta cagtgaattt gcaggttgat
    37861 cgttggcgtc agtaagtcat agatctcccg ctgtaacttg ctccatggac gttgtgtcat
    37921 tgtttttcct caagccggtg acgcgcttca ctgcgaattt tccgcgttgc cccggttttc
    37981 actaaatcac gtaactgcga aagatcgggt tgttcgacaa ataagagtaa tgatagatcc
    38041 tcttttaacc acgccgtttt cacatcggca gccagctgtg gattattaat agccagcgac
    38101 cgatcctgag actcagtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat
    38161 gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct
    38221 gatttatctt ttatcgtagc cagttccgca aaaaatacct ggagatcttc actgatattt
    38281 atatcaacct tgatgaatta tgaatatatc ggaaattgaa aaaatcgcag caaagga
//
LOCUS       DAZPLI010000025        37811 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZPLI010000025 DAZPLI010000000
VERSION     DAZPLI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.025"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHUU3_001203"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHUU3_001203"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR2081330.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="ACHUU3_001204"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="ACHUU3_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR2081331.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHUU3_001205"
     CDS             complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHUU3_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR2081332.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHUU3_001206"
     CDS             complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHUU3_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR2081333.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHUU3_001207"
     CDS             complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHUU3_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR2081334.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5280..5738
                     /locus_tag="ACHUU3_001208"
     CDS             5280..5738
                     /locus_tag="ACHUU3_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR2081335.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5788..6630
                     /gene="purU"
                     /locus_tag="ACHUU3_001209"
     CDS             5788..6630
                     /gene="purU"
                     /locus_tag="ACHUU3_001209"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR2081336.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6790..6874
                     /locus_tag="ACHUU3_001210"
     tRNA            6790..6874
                     /locus_tag="ACHUU3_001210"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6824..6826,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="ACHUU3_001211"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="ACHUU3_001211"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="ACHUU3_001212"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="ACHUU3_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR2081337.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="ACHUU3_001213"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="ACHUU3_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR2081338.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="ACHUU3_001214"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="ACHUU3_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR2081339.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="ACHUU3_001215"
     CDS             complement(10156..13899)
                     /locus_tag="ACHUU3_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR2081340.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="ACHUU3_001216"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="ACHUU3_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR2081341.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="ACHUU3_001217"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="ACHUU3_001217"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR2081342.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="ACHUU3_001218"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="ACHUU3_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR2081343.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="ACHUU3_001219"
                     /gene_synonym="iatA"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="ACHUU3_001219"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR2081344.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="ACHUU3_001220"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="ACHUU3_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR2081345.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="ACHUU3_001221"
     CDS             complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="ACHUU3_001221"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR2081346.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="ACHUU3_001222"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="ACHUU3_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR2081347.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="ACHUU3_001223"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="ACHUU3_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR2081348.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23294..23401
                     /locus_tag="ACHUU3_001224"
     CDS             23294..23401
                     /locus_tag="ACHUU3_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR2081349.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            23830..23937
                     /locus_tag="ACHUU3_001225"
     CDS             23830..23937
                     /locus_tag="ACHUU3_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR2081350.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            24365..24472
                     /locus_tag="ACHUU3_001226"
     CDS             24365..24472
                     /locus_tag="ACHUU3_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR2081351.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(24621..25475)
                     /gene="kdsA"
                     /locus_tag="ACHUU3_001227"
     CDS             complement(24621..25475)
                     /gene="kdsA"
                     /locus_tag="ACHUU3_001227"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR2081352.1"
                     /translation="MKQKVVSIGDINVANDLSFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(25511..26321)
                     /gene="sirB1"
                     /locus_tag="ACHUU3_001228"
                     /pseudo
     CDS             complement(25511..26321)
                     /gene="sirB1"
                     /locus_tag="ACHUU3_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
     gene            complement(26325..26717)
                     /gene="sirB2"
                     /locus_tag="ACHUU3_001229"
     CDS             complement(26325..26717)
                     /gene="sirB2"
                     /locus_tag="ACHUU3_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR2081353.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(26714..27547)
                     /gene="prmC"
                     /locus_tag="ACHUU3_001230"
     CDS             complement(26714..27547)
                     /gene="prmC"
                     /locus_tag="ACHUU3_001230"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR2081354.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(27547..28629)
                     /gene="prfA"
                     /locus_tag="ACHUU3_001231"
     CDS             complement(27547..28629)
                     /gene="prfA"
                     /locus_tag="ACHUU3_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR2081355.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(28671..29927)
                     /gene="hemA"
                     /locus_tag="ACHUU3_001232"
     CDS             complement(28671..29927)
                     /gene="hemA"
                     /locus_tag="ACHUU3_001232"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR2081356.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            30141..30764
                     /gene="lolB"
                     /locus_tag="ACHUU3_001233"
     CDS             30141..30764
                     /gene="lolB"
                     /locus_tag="ACHUU3_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR2081357.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            30764..31615
                     /gene="ispE"
                     /locus_tag="ACHUU3_001234"
     CDS             30764..31615
                     /gene="ispE"
                     /locus_tag="ACHUU3_001234"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR2081358.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            31766..32713
                     /gene="prs"
                     /locus_tag="ACHUU3_001235"
     CDS             31766..32713
                     /gene="prs"
                     /locus_tag="ACHUU3_001235"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR2081359.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            32837..34516
                     /gene="dauA"
                     /locus_tag="ACHUU3_001236"
     CDS             32837..34516
                     /gene="dauA"
                     /locus_tag="ACHUU3_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR2081360.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(34571..34849)
                     /gene="ychH"
                     /locus_tag="ACHUU3_001237"
     CDS             complement(34571..34849)
                     /gene="ychH"
                     /locus_tag="ACHUU3_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR2081361.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            35126..35710
                     /gene="pth"
                     /locus_tag="ACHUU3_001238"
     CDS             35126..35710
                     /gene="pth"
                     /locus_tag="ACHUU3_001238"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR2081362.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            35827..36918
                     /gene="ychF"
                     /locus_tag="ACHUU3_001239"
     CDS             35827..36918
                     /gene="ychF"
                     /locus_tag="ACHUU3_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR2081363.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT         9413 a         9791 c         9238 g         9369 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactactggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     1981 gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg tttcgtggat aacaccgata
     2041 cggatgttac ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag
     2101 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     2161 gtcatggctc ttccctttca tatgataggc ttccaccgtt tctttttcga tcagcatatc
     2221 aattgcgtcg gtgagctgaa tttcatcacc agctcccgga ggggtttttg ccagcaacgg
     2281 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     2341 cgctttcggt ttttctacca caccaaccat cggtacgctt tcacccggcg ctaattcaac
     2401 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     2461 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgacaaatc
     2521 ggattcatat tcatccagaa taacatcagg caaaataaca gctaccggtt catcacccac
     2581 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagaccct gacgaacttg
     2641 cataatagtc acgtgcggtg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     2701 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     2761 tttagatgag tgtgtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     2821 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     2881 cgccggcaac atcctggttc ctaatcccgc aacggggata acggcttttt tgactttcgt
     2941 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     3001 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     3061 tagcataatt aagacaatta cttatacgaa tggcgacgat agtaccacca gccgacatta
     3121 gcaggtaatg caaatttagc ccgcgttatc gtttgctcat tctgcagaca acatcaagcg
     3181 cagtcgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     3241 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctggtg
     3301 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     3361 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa
     3421 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     3481 ctgttcctgt aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt
     3541 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     3601 aatatcaagc accaggccgg gtttgtccgc ggcaaccaat tgacgataat taacccggca
     3661 atgggaaatc acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcggc
     3721 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga
     3781 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     3841 aactggtttc agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat
     3901 attttcagtg gcagatatca ccagaactgg ggtctggtcg cctctgttac gtatatgctc
     3961 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     4021 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     4081 caatgaggaa aaccatgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag
     4141 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     4201 gtggcatgct attgccacct gcgcctgtca gaattaactt aagtgtagat aaaaattctg
     4261 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcctttcc actgccagct
     4321 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     4381 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct
     4441 ccaacacctg gatagacgtg gtcataatct ctgttgccgt tggcgctgtc acagcccgac
     4501 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggtaga gaatcaccat
     4561 tctcgctatt ttcttcactg acattaaagg agagcagatc ttgttgcatc aaatgagcat
     4621 cgtgctgcag gtcgaccgct atcacaatat cagcccccaa tgcacgcgta agagaaatag
     4681 gaattgggtt aactactgcc ccatcaacca gccagtagcc gttatgtgca acgggtgcca
     4741 tgagtcctgg aatactgcat gatgcgcgaa tagcaagatg gagatcgcct tcagtaaacc
     4801 ataattcacg ccccgtactt aaattggtgg caacagccgc aaagcgacgg gaacaatttt
     4861 cgatctctgt ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta
     4921 acccaccgcg ctgccaggag aggtccatca ggcgtaaaac gtcccaataa ctgaaagagg
     4981 ttacccaatc ttccagcgca gataatcgat cgcatgcata ggccgcgccc accagcgaac
     5041 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaataa
     5101 cgccaatatg cgaccaacct ctcgccgcgc cagatcccag cgccagccct atttttatct
     5161 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
     5221 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
     5281 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
     5341 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
     5401 ttgtgatgca agacgcagat tatttaataa agacctggca tccctcttgt ggagcagcgg
     5461 cgttacgtgc cgaattgatg gccggatttg cgcatactga atggttggga ttgaccgtat
     5521 ttgaacattg ttggcaggac gccgacaata tcggatttgt cagttttgtt gctcgattta
     5581 ccgaaggtgg caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
     5641 aatggtacta tattgacggt acacgtccgc agtttggtcg caacgatccc tgcccttgtg
     5701 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
     5761 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
     5821 atttgtccgg accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag
     5881 ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc
     5941 acggaactgg aagggatttt taatgattcc accctgctgg cggatctcga tagcgcattg
     6001 ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc
     6061 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
     6121 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcgtttt
     6181 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
     6241 atggcggaag ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
     6301 gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa tcatcaatat tcaccattca
     6361 ttcctgcctg cgtttatcgg cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag
     6421 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
     6481 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
     6541 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt
     6601 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttacctttaa
     6661 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
     6721 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
     6781 ttaccccgtg gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc
     6841 gacttcgaag gttcgaatcc ttcccccacc accatcactt ttcaaaagtc cgtaaactct
     6901 caaacgaatc cgcaattaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttaaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcggcc acggtcgaac agttcgctgt actgggcctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcaatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagtgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccattgc cattttataa accggcgact
     9181 gctgtgcggc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccacccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt
     9901 tacccagcaa catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagagc accgttgctg ttgaatactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgagga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagtgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccattaccg attttctcca tcaacgggcc aatagaggta aagcgttcgt aagtcgccgg
    11221 ataatcgcgc tctacgacca taatgtgtgg cgcggtttta cctgggatca ggtcgcactc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcatgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgcttttc gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaagtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtgctcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaggaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagccacca cataacagac tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcagatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taatatgagt
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcgacatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaggtaat cactacgcag
    14341 gcgatataga aaatcaaaaa taccttcatt gcgccgactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatag cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgtc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gataaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa gccgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttacggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtaa tgacagctcc agtagccctt
    15661 tcgggggcgg atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgta gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt aaaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccgggtat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatgtatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aagaggtttg
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gtgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttaa ctcgagcagc agaacgagac gaacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgca ctgatgcgtg cacaaaaccg
    16381 cgaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgcattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagt catcgcgatt ttacccaacg
    16621 cgcgaacatc agcgggcgca acgaaatggc gatgcttgga actgcattga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc tgtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaattgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actgctggat accctggttg aacaactcac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggagg cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgcct ggtgccttcg catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaagtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tggtattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggttatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc accgacaatc gccagtgatt gctcgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgga tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tatcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg cacaccaaag cggtaattaa gattcagtcc gaggttattt
    18961 tgattttcgc cactttcacc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc agagttaaac
    19081 aaatcaacac gctcaccaaa atactgttct accctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgggc tgtcaggtca tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgatag tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtccag caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccatcatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg gttttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgccgcca gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aaggtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgcaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctcttattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgtttg
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccgtaaca
    20461 caccatccgg ataattctcc gccagcagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcgt
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcataatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcattagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccggaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagcagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtatt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccagacc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc gtgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aatgccagcc cgctggagac attgctcgac agcatgaatg cccctgtcgc
    22561 ctttactggc ttcctggtgg ctctgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg tccagcgcgc gatgaatctg ttctttggtt cagtgttagc
    22681 aaccatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 acagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggggagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23161 ggtggcattg gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac
    23221 aaaacggggg ctaatcttga aaccagacac ttcaagaata gccgccttgt tgtcattaca
    23281 acaaggcggc tatatgacgc tcgcgcagtt tgccatgact ttctggcacg acctggcagc
    23341 accgatcctg gcggggatta ttaccgcagc gattgtcagc tggtggcgta accggaagta
    23401 aggtgaaaac gggcgtcagg ctgccgtgat ggcaatatgc gcccgaaacc ggccgcaggg
    23461 gtaacagcct gcggccattt ttgtctttat ctcttccaca agtaaatatt ggcaacccga
    23521 gctggaagca tcatccagag cactcatgaa acgactgtaa cagctgttga aaaaccggaa
    23581 tgcactcttc attgcaaaca tttcagggat gaagtgaaga tgaattgagt cgtagtatcc
    23641 acaccaagcc ggcgttatcc ggtgaggcgc aatgttgcgg gggcttaatc cctagtggca
    23701 ttggttgctg gaaagagaaa acccccgcac gttgagaggt aaaacctgac aacagaacgg
    23761 gggctaatct tgaaccatca acaattcaag aatagccgca aaatgttgtc attacaacaa
    23821 ggcggctata tgacgctcgc gaagtttgcc atgattttct ggcacgacct ggcagcaccg
    23881 atcctggcgg ggattattac cgcagcgatt gtcggctggt ggcgtaaccg gaagtaaggt
    23941 gaaaacgggc gtcaggctgc cgtgatggca atatgcgccc gaaaccggcc gcaggggtaa
    24001 cagcctgcgg ccatttttgt ctttatctcc tccacaagta aatattggca acccgagctg
    24061 gaagcatcat ccagagcact catgaaacga ctgtaacagc tgttgaaaaa ccggaatgca
    24121 ctcttcattg caaacatttc agggatgaag tgaagatgaa ttgagacgta gtatccacat
    24181 caagccggcg ttatccggtg aggcgcaatg ttgcgggggc tttatccctg gtggcattgg
    24241 ttgctggaaa gagaaaaccc ccgcacgttg agaggtttaa cctgacaaca gaacgggggc
    24301 taatcttgaa ccagaacaat tcaagaatag ccgcaaaatg ttgtcattac aacaaggcgg
    24361 ctatatgacg ctcgcgcagt ttgccatgat tttctggcac gacctggcag caccgatcct
    24421 ggcgggaatt attaccgcag cgattgtcgg ctggtggcgt aaccggaagt aaggtgaaaa
    24481 cgggcgtcag gctgccgtga tggcaatatg cgcccgaaac cggccgcagg ggtaacagcc
    24541 tgcggccttt tttgtatctg gcacccatga aaaaagtctt aacgcagaac gctaagactt
    24601 tatttttcaa gcaaaaaaga ttacttgctg gtatccagtt cttcgaaacc tttcaccaga
    24661 tcatcaatcg ctttcatctg cttgaggaac ggttccagtt tagccagcgg cagcgcggat
    24721 ggaccatcac atttcgcatg ttccggatcc ggatgcgctt caataaacag ccccgccaga
    24781 cctaccgcca taccggctcg tgccagctca gccacctgag cacgacgacc accggaagct
    24841 gcgccaaacg gatcgcggca ttgcagtgcg tgggtcacgt cgaaaatcac cggcgagtta
    24901 ccagacactt tcttcataat gctgaagccc agcatatcga caaccaagtt gtcatagccg
    24961 aagttagcac cgcgatcgca aagaatcact ttttcgttgc cgccttcttt gaatttatca
    25021 acgatattac ccatctgtcc cgggctgaca aactgtggtt tcttgacgtt aattaccgca
    25081 ccggttttcg ccatggcttc aaccaggtca gtctggcgag caagaaacgc cggcaactga
    25141 atcacatcca cgacatcagc aacgggctgt gcctgacttg gttcgtgaac gtcggtgata
    25201 attttcacgc caaaagtctg cttcaactcc tggaagattt tcatcccttc ttccaggccc
    25261 ggtccacgat aagagtggat ggaggagcgg ttggctttgt caaaagaggc tttgaacacg
    25321 taagggatac ccagtttctg ggtcacggtt acgtagtgct cgcaaatgcg catcgccaga
    25381 tcgcgagatt ccagcacgtt cataccacca aacagtacga acgacaggtc atttgctacg
    25441 ttgatgtcgc caatgctaac cactttttgt ttcataggat cgccttaatc ttgagtaaaa
    25501 tgtcgattaa ttaatgcagc acaatatgtt tatgcgcgat gttatttatt tgcgcacgga
    25561 tcatttcgct gatcgggtct tccggacact gttcaacgaa ataacttaaa tcgttcaacg
    25621 caacgtgttc gcaatccagt tgcgcataaa tcaacccgcg atcgcgaatt tcatagggat
    25681 cttccgggtt gaattgtaat aaagcttcgc tggtgcgtaa cgccagctcc atctgatttt
    25741 cttccatcaa cgatggcttt gagtgtatcc aacaatttgc ggattacctc aatgttatca
    25801 gcttcatcaa ggtcttcata aaacagttcc gccgacgggc tgatatttcc ctttaaccat
    25861 acgtccagca tatgttcgct taacgattca ccgttaaaag gattaatcag ccaaatttcg
    25921 ccatccggac attcaatgcg caatatcagc tgcgtaggga aaatcaccgg cagcagcggc
    25981 aaatcgagac gattcgcgac ccataataaa accgcaccta atgataccgc actgccctgt
    26041 cgattcttta acacctggtc cagccataat gcatcggaaa gacgataaac gccgcgtgag
    26101 gctttaaatc cccagtcgcc gtaaaacagc gcgatcagtt tttccagctg ctcttctaaa
    26161 ggcagaagct ggctgatttc ttccttcgcc agactaacga gacgctccag ttcgtcgtaa
    26221 acatcttgcg agggaaaatc gcggcggatt gcttcgcaag ccaggatcat gccttcgcac
    26281 aatggcgctt tattaaattc gaaatcagct aacgatctca tgacttaccc cagtaacggt
    26341 acttttgtgg tggcgagttt aatgatgatg tacagcacca ccaacgccag cgggaaggca
    26401 ataatgcgcg cctgctgact gtgcatacga cgataatcga gtgcaataaa acccaaaacg
    26461 atataaatga taactccaaa cagcttttca gtcagccact gtgcctgccc ggaaaatggc
    26521 aggatgtgcg ctttagccat caacgcaatg ccgcttaaca ataacaccgt atcgacaacc
    26581 ggcggcacaa tgcgcgtcca gcgagcaaat gcctgtggat gcttctgata acgtagccag
    26641 aaacgtaagg ttaatagccc aacagaaagc gcgatactaa taagatgaac actaagcagt
    26701 gtagaaaaac ttgtcattga taatagcggc cgagcgttac gcgctcgtta tcaccatagt
    26761 cacggcaggt ttcgacgtca tgataccccg cgaggataaa tgcttgtcgc accgcttcgc
    26821 cctgctgcca gccatgttcc agaagcagaa agccgccgga taccagcgcg ttacgcgact
    26881 gttcgatgat atgcacgatg tctgccattc cactgtctgc cgcaaccagc gcagtgagcg
    26941 gctcaaagcg gacatcgcct tgttgaagat gtgggtcctg ctcgtcaata tacggcggat
    27001 tgctgacaat catcgcaaac tgctgcccgg ctagcgcgct aaaccagtcg ctttgcagaa
    27061 tgtggatatt tttgatcgcc agatgctggg cattacgttg tgccagggag acagcatcag
    27121 gcatacgatc tacagcgata atttcgcagt ccgggcgctc gctagccagc gcaagcgcaa
    27181 tcgccccggt acccgtcccg agatcgagaa tacggcaagg ttgttcaggc aaccgcgcca
    27241 gtgcctgctc caccagacac tccgtatccg ggcgcggaat taaggtcgct ggcgaaacaa
    27301 ataacggcaa cgaccagaat tctcgcaccc cggttaaatg agcaatgggt tcaccatcgc
    27361 gacgacgtgt cagtagcgca tcaagttgct gacattgttc gtcagtcagc tgcgtttcac
    27421 caaaggccag aataaaagtc cgccccctgc cagtgacata ctccaataag atttcagcgt
    27481 cacggcgtgg gctttcgctc gcctgaagtt ggcttattgc ttcacgtaac cagtattgat
    27541 attccattat tcctgctcgg acaacgccgc cagttggtcg gcctgatgtt cctggataat
    27601 cggttcaatc agcatatcca gcttaccttc catcacttca tccagacggt agagcgtcag
    27661 gttgatgcgg tgatcggtaa cgcgcccctg cgggaagttg taagtacggt tacggtcgct
    27721 gcgatcgcca ctccccagca ggttacgacg ggtagacgct tcggcctgtt gccgttttgc
    27781 catttctgca gcgtggatac gagcaccaag tacagaaagt gctttagctt tgtttttatg
    27841 ttgtgaacgt tcgtcctgac attcaacaac aatcccggtc ggcaagtgag taatacgaat
    27901 tgccgaatcg gtggtgttaa cgtgctgacc acccgcccct gacgagcgga aagtatcaat
    27961 gcgtaaatct gctgggttga tgtccggcag ttctgcgtca ggcagttctg gcattaccgc
    28021 aacggtacaa gcagaagtat gaatacgacc ctgcgattcc gtagcaggaa cacgctgcac
    28081 gcgatgaccg ccggattcaa atttcagacg accatacaca ccatcaccgc taattttggc
    28141 gatgatctct ttataaccac catgttcacc ctcgctggcg ctcatgattt ctacccgcca
    28201 gcggcgggct tcggcataac ggctgtacat acggaacaga tcgcccgcga acagcgccgc
    28261 ttcgtcgccg ccggttccgg ctcggacttc gaggaaggcg ttacgttcgt catcaggatc
    28321 ttttggcagt aacagaacct gtaattgctg ttccagttgc tcgctttttt ctttagcttc
    28381 gcgcagttca tcctgcgcca tctcacgcat ttcaggatca tcgagcatca tctgtgcggt
    28441 ttcgatatct tcctgaacct gttgccagtc ggtaaaacag cgcgaaacat cacttaactg
    28501 cgcatattcg cgtgataatg cgcgaaaacg ttcctggtcg gcgatagttt gcgcgtcacc
    28561 cagcaacgcc tgaacttctt catggcgttc atgcagggct tccagtttgg caacgataga
    28621 aggcttcata ggcgtaaatg caccctgtaa aaaaagaaaa tgatgtactg ctactccagc
    28681 ccgaggctgt cgcgcagaat attcaggcgt tcgttatccc cgtcacgggc ggcctgttga
    28741 agtgatttcg ttggcgcatg gatcaagcgg ttagtcagtt tccatgccag atcctgcata
    28801 atggcttgcg cgtcgccgcc ctgctcaagg gccgctaacg ctttggcggt taactcatcg
    28861 cgaacgtgct ctgcctggct gcgatactcg cgaatggttt cgctggcgct ttgtgctcgc
    28921 agccacgcca taaattcgct ggtttcctga gcgacaatag tttccgcctc aaccgctgcg
    28981 gctttacgct gcgccaggtt gtgcgaaatg atgctttgca gatcgtccac actataaaga
    29041 taagcattcg ccagtttgcc aacttccggc tcaacatcgc gcggaacggc aatatccacc
    29101 aacagcattg gttggttacg acgacttttt aatgcgcgct ccaccatgcc tttcccgata
    29161 atcggtaacg ggctggcggt ggaactgatg atgatatcgg cttcgcgcag acgttcgtcg
    29221 atctcactca gggcaatcac ttccgcgcca acttcatctg ccagaatttg ggcacgttcg
    29281 cgagtgcggt tggcgataat catcttctgt actttatgtt cgcgcagatg acgcgctacc
    29341 agctcgatgg tttcgcccgc gcctaccagc aacactgtga ccgtagagag cgattcaaag
    29401 atctgccgcg ccagcgtaca agccgcaaaa gcgacagaca cagcactggc accgatatct
    29461 gtttcagtgc gaacgcgttt cgctacagag aaagatttct ggaacatgcg ttccagttcg
    29521 ctggccttca tatgaccttt ttgcgaatcg gcaaacgctt ttttaacctg accgaggatc
    29581 tgcggctccc caagaaccag tgaatccagg ccgctggcaa cacgcattaa atggctaacc
    29641 gcgtcgttat cctgatgcca gtagaggctt ttacgcagat cttcttcatt aagattgtga
    29701 taatcgcaaa gccagcggat taacgcctct tgcaggttat cctgctcttc aacgctaaga
    29761 taaagttccg tgcggttgca cgtcgacagc accacgccgc cctgcaccat cggctgcgca
    29821 agcaggctgt caagcgcctg atcgagctta tccggcgaaa acgatacacg ttctcgcagc
    29881 gatacaggtg ccgttttatg gttgataccg agtgctaaaa gggtcatgtc tgcgggaaat
    29941 aataccaacg ttgatatggt tagtctgctt gcatcataca ggatgcgtaa gatcaataaa
    30001 agagagcgcc cccttttgga gtaattgccg aaagccgtta gattctggca attaagacaa
    30061 cttgaacata gacgatagcg gacggtaacg ctagcattaa gggttataac tgcaacgtat
    30121 ctcaaggact tgtcatcact atgcccctgc ccgattttcg tcttatccgc ctgctaccgc
    30181 tggctgctct tgtgctcact gcctgttccg ttaccacgcc caaaggtcct ggcaaaagcc
    30241 cggattcgcc acaatggcgt cagcatcagc aagacgtgcg caatcttaat cagtatcaga
    30301 ctcgcggcgc gttcgcttat atttctgacc aacaaaaagt gtacgcccgc tttttctggc
    30361 agcaaaccgg ccaggatcgc taccgtctgc tgctcactaa cccattgggc agcacggaac
    30421 tggagctgaa tgctcaaccg ggtaacgtgc agttagtcga caataaaggt cagcgttata
    30481 ccgccgatga cgccgaagag atgattggca aattgaccgg aatgccaatt ccgctcaaca
    30541 gcttgcgcca atggatttta ggtttaccgg gtgatgcaac cgactacaaa ctggacgacc
    30601 agtaccgcct gagcgaaatt acctacagcc agaatggcaa aaactggaag gttgtttatg
    30661 gtggttatga caccaaaacg caacctgcga tgccagccaa tatggaactc accgacggtg
    30721 gtcaacgcat caagttaaaa atggataact ggatagtgaa ataatgcgga cacagtggcc
    30781 ctctccggca aaacttaatc tgtttttata cattaccggt cagcgtgcgg atggttacca
    30841 cacgctgcaa acgctgtttc agtttcttga ttacggcgac accatcagca ttgagcttcg
    30901 tgacgatggg gatattcgtc tgttaacgcc cgttgaaggc gtggaacatg aagataacct
    30961 gatcgttcgc gcagcgcggt tgttgatgaa aactgcggca gacagcgggc gtcttccgac
    31021 gggaagcggt gcgaatatca gcattgacaa gcgtttgccg atgggcggcg gtctcggcgg
    31081 tggttcatcc aatgccgcga cggtcctggt ggcattaaat catctctggc aatgcgggct
    31141 aagcatggat gagctggcgg aaatggggct gacgctgggc gcagatgttc ctgtctttgt
    31201 tcgggggcat gccgcgtttg ccgaaggcgt tggtgaaata ctaacgccgg tggatccgcc
    31261 agagaagtgg tatctggtgg cgcaccctgg tgtaagtatt ccgacaccag tgatttttaa
    31321 agatcctgaa ctcccgcgca atacgccaaa aaggtcaata gaaacgttgc taaaatgtga
    31381 attcagcaat gattgcgagg ttatcgcaag aaaacgtttt cgcgaggttg atgcggtgct
    31441 ttcctggctg ttagaatacg ccccgtcgcg cctgactggg acaggggcct gtgtctttgc
    31501 tgaatttgat acagagtctg aagcccgcca ggtgctagag caagccccgg aatggctcaa
    31561 tggctttgtg gcgaaaggtg ttaatctttc cccattgcac agagccatgc tttaagccgg
    31621 gcaagctgag tttcggtgac aacgtcacct tgttccagac gttgcatcgc gctctttaat
    31681 acaccgcctg gaaaggatca tgcctggccc gcacagtttt cggcagattc tttccaccaa
    31741 tggacgcatg cctgaggttc ttctcgtgcc tgatatgaag ctttttgctg gtaacgccac
    31801 cccggaacta gcacaacgta ttgccaaccg cctgtacact tcactcggcg acgccgctgt
    31861 aggtcgcttt agcgacggcg aagtcagtgt acaaatcaac gaaaatgtac gcggtggtga
    31921 tattttcatc atccagtcca cttgtgcccc tactaacgac aacctgatgg aattagtcgt
    31981 tatggttgat gccctgcgtc gtgcttccgc aggtcgtatc accgctgtta tcccctactt
    32041 tggctatgcg cgccaggacc gtcgcgtccg ttccgctcgt gtaccaatca ctgcgaaagt
    32101 ggttgcagac ttcctctcca gcgtcggtgt tgaccgtgtg ctgacagtgg atctgcacgc
    32161 tgaacagatt cagggtttct tcgacgttcc ggttgataac gtatttggta gcccgatcct
    32221 gctggaagac atgctgcagc tgaatctgga taacccaatt gtggtttctc cggacatcgg
    32281 cggcgttgtg cgtgcccgcg ctatcgctaa gctgctgaac gataccgata tggcaatcat
    32341 cgacaaacgt cgtccgcgtg cgaacgtttc ccaggtgatg catatcatcg gtgacgttgc
    32401 aggtcgtgac tgcgtactgg tcgatgatat gatcgacact ggcggtacgc tgtgtaaagc
    32461 tgctgaagct ctgaaagaac gtggtgctaa acgtgtattt gcgtacgcga ctcacccgat
    32521 cttctctggc aacgcggcga acaacctgcg taactctgta attgatgaag tcgttgtctg
    32581 cgataccatt ccgctgagcg atgaaatcaa atcactgccg aacgtgcgta ctctgaccct
    32641 gtcaggtatg ctggccgaag cgattcgtcg tatcagcaac gaagaatcga tctctgccat
    32701 gttcgaacac taatcgaacc cgctcaaaga cccgctgcgg cgggtttttt tgtctgtaat
    32761 atccatttgt atgacctatg cctccttcac ctgccattta gttgacagat gatgcgctca
    32821 cggatgaaac attattgtga acaaaatatt ttcctcacat gtgatgcctt tccgcgctct
    32881 gatcgacgct tgctggaaag aaaaatatac tgccgcacgg tttacccgtg acctgattgc
    32941 cgggataacc gtcgggatta ttgctatccc gctggcgatg gcgttggcta ttggtagtgg
    33001 tgtggcaccc cagtacggtt tatataccgc agctgttgcg gggattgtca ttgctctgac
    33061 gggtgggtca cgctttagcg tttccggtcc gactgcggca tttgtggtaa ttctctatcc
    33121 cgtgtcgcaa cagtttggac tggcaggact gctggttgcg accttgctgt cggggatctt
    33181 tttgattctg atgggtctgg cacgctttgg tcgcctgatt gagtatattc cggtttccgt
    33241 caccttaggt ttcacctcgg gtatcgggat caccatcggt accatgcaga ttaaagattt
    33301 tctcggtctg caaatggccc atgtcccgga acattatcta caaaaagtcg gcgcattatt
    33361 tatggcgctg ccgaccatta atgtgggtga tgctgccatt ggcattgtga cgctaggtat
    33421 tcttgttttt tggccgcgtc tgggcattcg tttacccggt caccttccgg ccttgctggc
    33481 tggttgcgcg gtgatgggga ttgttaacct gctcggcgga catgttgcta ccatcggttc
    33541 gcaattccac tacgtcctgg ccgatggttc tcagggtaac ggtattccgc aactgctgcc
    33601 gcaactggtg ctgccgtggg atctgcctaa ttcagaattc acgctaacct gggattctat
    33661 tcgcacactg ctgcctgcgg cattctcaat ggcaatgctc ggcgcaatcg aatctctgct
    33721 ctgcgccgtg gtactggatg gtatgaccgg gacgaaacac aaggcgaaca gcgaactggt
    33781 tggacaggga ctggggaata ttatcgctcc gttctttggt ggtattaccg ctacagctgc
    33841 catcgcgcgt tctgccgcta acgtccgtgc cggggcaact tcccctatct cggcggtgat
    33901 ccactctatt ctggttattc ttgccctgct ggtactggca ccgctgctct cctggctgcc
    33961 gctttccgct atggcagccc tgctgttgat ggtggcgtgg aacatgagtg aagcgcataa
    34021 agtggtcgac ttgctgcgtc atgcaccgaa agatgacatc attgtcatgc tgctgtgcat
    34081 gtcgctgacc gtgctgtttg atatggttat tgccatcagc gtggggatcg tgctggcatc
    34141 gctgctgttt atgcgtcgta tcgcacgtat gactcgcctg gcaccggtag tcgtagatgt
    34201 tccagacgat gttctggtac tgcgcgttat tggcccgctg ttttttgctg ctgctgaagg
    34261 cttgttcacg gacctggagt cacgtcttga aggcaaacgg attgtgattc tgaagtggga
    34321 tgccgttccg gtacttgatg ctggtggtct tgatgcgttc cagcgttttg tgaagcgtct
    34381 gcccgaagga tgtgaactgc gcgtgtgcaa cgtggaattc cagccactgc gcactatggc
    34441 tcgcgcaggc attcaaccga tcccgggacg cctcgcgttc ttcccgaatc gtcgcgcggc
    34501 gatggcggat ttataagtac gagattgacc agtcagcgca aactgactgg tcagcaaact
    34561 gcattatttg ttagctatga cggcgattat cgctacggcg gcaacgtttg tcatagtgac
    34621 gaacccacca gtaacggtcg cacacctgtt catgcccgcc aacacgggca cccgccagcc
    34681 agagaatggc accgacgaaa atacttagca ctgcaccatg tgcgaaatac tggggcatat
    34741 taaactgtgg taactggttg aggattgaat accccacgcc gaccaccatt accaccagcc
    34801 ccaaccccat gagcacgtta ccgagtaacg aagcgttttt gcgtttcata tgtcacctcc
    34861 ggaactttct gggttgtaac agggaatacc cctcttcctt atgtgtaaag tatagacaac
    34921 accctgtggg ttatgtgcgg gcgtgatcac aattacaacc cttatttcaa caaaacttta
    34981 caaataaacg cctgaacacc ttgactttct aatagtccac tcaggcaatt attacgttat
    35041 ctgcatttga tacgcagttt tttgtcaagc ggggccgcaa ccagtaaact acgcgccagt
    35101 tatgtacaca ctcaggacaa aaaacgtgac gattaaattg attgtcggcc tggcgaaccc
    35161 cggtgctgaa tacgccgcaa cgcgacataa tgctggtgcc tggttcgttg acttactggc
    35221 agagcgtttg cgcgctccgc tgcgcgaaga ggctaaattc tttggttata cttcgcgagt
    35281 cactcttgga ggcgaagatg tccgcctgtt agtcccgact acatttatga atctcagcgg
    35341 caaagccgtt gcggcgatgg ccagtttttt ccgcattaat ccggacgaaa ttctggtggc
    35401 ccacgacgaa ctggatctgc ctcctggcgt cgccaaattt aaattgggcg gtggccatgg
    35461 tggtcacaat ggactgaaag acatcatcag taaattgggt aataacccta actttcaccg
    35521 tttacgcatc ggaatcggtc atcctggcga taaaaataaa gttgtcggtt ttgtgttagg
    35581 caaaccgcct gttagtgaac agaagttaat tgatgaagcc atcgacgaag cggcgcgttg
    35641 tactgaaatg tggtttacag atggcttgac caaagcaacg aaccgattgc acgcctttaa
    35701 agcgcaataa gtcgttgtct gcggcatttt tgccgagtgc cgtgtataat aggcaaagtt
    35761 atttccattt ctgcaatctg ttagcaataa cagattgatt attaagatat taaggtgatt
    35821 taaatcatgg gattcaaatg cggtatcgtc ggtttgccca acgtcgggaa atctaccctg
    35881 ttcaacgcgc tgaccaaagc cggtattgaa gcggccaact ttccattctg caccattgag
    35941 ccgaacacag gcgtcgtacc aatgcctgac cctcgcctgg atcaactggc tgaaatcgta
    36001 aaaccgcaac gtacgcttcc cacgaccatg gaatttgtcg atatcgccgg tctggtaaaa
    36061 ggcgcatcga aaggcgaagg tctgggtaac cagttcctga ccaacatccg tgaaaccgaa
    36121 gcgatcggtc acgttgttcg ctgctttgaa aatgacaaca tcattcacgt ttccggcaaa
    36181 gttaacccgg ctgacgatat tgaagttatc aacaccgaac tggcgctggc agacctcgac
    36241 acctgcgaac gtgcgattca tcgcgtacag aagaaagcca aaggtggcga taaagacgcg
    36301 aaagctgagc tggcggtcct agaaaaatgc ctgccccagc tggaaaacgc aggtatgctg
    36361 cgcgcgctgg atttaagcgc tgaagagaaa gcggctattc gttacctgag cttcctgacg
    36421 ctgaaaccaa caatgtacat cgccaacgtc aacgaagacg gttttgaaaa caacccatat
    36481 cttgaccagg tgcgtgaaat cgcggcgaaa gaaggttctg ttgtggttcc ggtttgtgct
    36541 gctgttgaag cagacattgc cgaactggac gacgaagaac gtgacgagtt tatgcaggag
    36601 cttgggctgg aagaaccggg cctgaaccgt gtgatccgtg tcggttataa gctgctgaac
    36661 ctgcaaactt acttcaccgc tggggtgaaa gaagtgcgtg catggaccat tccggttgga
    36721 gcaaccgcgc cgcaggcagc gggcaaaatc catactgatt ttgaaaaagg ctttatccgt
    36781 gcacaaacca tctcgtttga agatttcatc acttacaaag gtgaacaagg cgcgaaagaa
    36841 gcaggcaaaa tgcgtgcaga aggtaaagat tacatcgtta aagatggcga tgtgatgaac
    36901 ttccttttca acgtctaatt aatttattgt ctcatgaggt ttcgctaaat ctcatgaaga
    36961 gcgcaaaagt atataaaatc cacgcaattg cgtggatttt tcatttcacc gagtcctaat
    37021 cattcttgta acatcctaca cttttgtgct tattgcctac agtgaaccac gagaataatt
    37081 aaagacatca gtgtcagatg acggatatct cattagtagt gatataacgt aagtttttgt
    37141 tcaactacac atcaccccct tcacagctga tcaatattga tcacgctcca atatgctggc
    37201 gagaaaagga cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt
    37261 acgtgcgatc ctggcaagat gaaaatgata accagtagca caataacaac gataggccag
    37321 aaaacccatt gaccaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa
    37381 aaatggcgca tattgttttt gttatgcaga tacatcaagc gaaattccca taactaatta
    37441 ggattattac aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc
    37501 catatttata gtaacgaatt ggaataagta acgacaactt agggaataac tcgatataaa
    37561 ctcacctttg taagggggat gttaaatata tattaattcc tgtgttcctg attttgcccg
    37621 gtaatagaga attattctct gagaagctca tcatgattgc accatacaaa tatcatgatg
    37681 ataagaggat attattcatt gatttaaaca aaatgacaaa atccatagac ccatagtctg
    37741 ggtactatat aggagtggta tgggcatcaa acaacacaat gggaatacca aagcagatcg
    37801 tctcgctgaa t
//
LOCUS       DAZPLI010000026        34615 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZPLI010000026 DAZPLI010000000
VERSION     DAZPLI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34615
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.026"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            134..1834
                     /gene="araB"
                     /locus_tag="ACHUU3_001240"
     CDS             134..1834
                     /gene="araB"
                     /locus_tag="ACHUU3_001240"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR2081364.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            1845..3347
                     /gene="araA"
                     /locus_tag="ACHUU3_001241"
     CDS             1845..3347
                     /gene="araA"
                     /locus_tag="ACHUU3_001241"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR2081365.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            3516..4211
                     /gene="araD"
                     /locus_tag="ACHUU3_001242"
     CDS             3516..4211
                     /gene="araD"
                     /locus_tag="ACHUU3_001242"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR2081366.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            4286..6637
                     /gene="polB"
                     /locus_tag="ACHUU3_001243"
     CDS             4286..6637
                     /gene="polB"
                     /locus_tag="ACHUU3_001243"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR2081367.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            6801..9707
                     /gene="rapA"
                     /locus_tag="ACHUU3_001244"
     CDS             6801..9707
                     /gene="rapA"
                     /locus_tag="ACHUU3_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR2081368.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            9719..10378
                     /gene="rluA"
                     /locus_tag="ACHUU3_001245"
     CDS             9719..10378
                     /gene="rluA"
                     /locus_tag="ACHUU3_001245"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR2081369.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(10495..11310)
                     /gene="djlA"
                     /locus_tag="ACHUU3_001246"
     CDS             complement(10495..11310)
                     /gene="djlA"
                     /locus_tag="ACHUU3_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR2081370.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            11565..13919
                     /gene="lptD"
                     /locus_tag="ACHUU3_001247"
     CDS             11565..13919
                     /gene="lptD"
                     /locus_tag="ACHUU3_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR2081371.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            13972..15258
                     /gene="surA"
                     /locus_tag="ACHUU3_001248"
     CDS             13972..15258
                     /gene="surA"
                     /locus_tag="ACHUU3_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR2081372.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            15258..16247
                     /gene="pdxA"
                     /locus_tag="ACHUU3_001249"
     CDS             15258..16247
                     /gene="pdxA"
                     /locus_tag="ACHUU3_001249"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR2081373.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            16244..17065
                     /gene="rsmA"
                     /locus_tag="ACHUU3_001250"
     CDS             16244..17065
                     /gene="rsmA"
                     /locus_tag="ACHUU3_001250"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR2081374.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            17068..17445
                     /gene="apaG"
                     /locus_tag="ACHUU3_001251"
     CDS             17068..17445
                     /gene="apaG"
                     /locus_tag="ACHUU3_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR2081375.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            17452..18294
                     /gene="apaH"
                     /locus_tag="ACHUU3_001252"
     CDS             17452..18294
                     /gene="apaH"
                     /locus_tag="ACHUU3_001252"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR2081376.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(18372..18851)
                     /gene="folA"
                     /locus_tag="ACHUU3_001253"
     CDS             complement(18372..18851)
                     /gene="folA"
                     /locus_tag="ACHUU3_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR2081377.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(19043..20905)
                     /gene="kefC"
                     /locus_tag="ACHUU3_001254"
     CDS             complement(19043..20905)
                     /gene="kefC"
                     /locus_tag="ACHUU3_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR2081378.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(20898..21428)
                     /gene="kefF"
                     /locus_tag="ACHUU3_001255"
     CDS             complement(20898..21428)
                     /gene="kefF"
                     /locus_tag="ACHUU3_001255"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR2081379.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(21536..22867)
                     /locus_tag="ACHUU3_001256"
                     /pseudo
     CDS             complement(21536..22867)
                     /locus_tag="ACHUU3_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(22926..23213)
                     /gene="fixX"
                     /locus_tag="ACHUU3_001257"
     CDS             complement(22926..23213)
                     /gene="fixX"
                     /locus_tag="ACHUU3_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR2081380.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(23210..24496)
                     /locus_tag="ACHUU3_001258"
     CDS             complement(23210..24496)
                     /locus_tag="ACHUU3_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR2081381.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(24547..25488)
                     /locus_tag="ACHUU3_001259"
     CDS             complement(24547..25488)
                     /locus_tag="ACHUU3_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR2081382.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(25503..26273)
                     /locus_tag="ACHUU3_001260"
     CDS             complement(25503..26273)
                     /locus_tag="ACHUU3_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR2081383.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            26746..28260
                     /gene="caiT"
                     /locus_tag="ACHUU3_001261"
     CDS             26746..28260
                     /gene="caiT"
                     /locus_tag="ACHUU3_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFR2081384.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            28291..29433
                     /gene="caiA"
                     /locus_tag="ACHUU3_001262"
     CDS             28291..29433
                     /gene="caiA"
                     /locus_tag="ACHUU3_001262"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR2081385.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            29562..30779
                     /gene="caiB"
                     /locus_tag="ACHUU3_001263"
                     /pseudo
     CDS             29562..30779
                     /gene="caiB"
                     /locus_tag="ACHUU3_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
     gene            30853..32406
                     /gene="caiC"
                     /locus_tag="ACHUU3_001264"
     CDS             30853..32406
                     /gene="caiC"
                     /locus_tag="ACHUU3_001264"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR2081386.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGDTLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            32515..33300
                     /gene="caiD"
                     /locus_tag="ACHUU3_001265"
     CDS             32515..33300
                     /gene="caiD"
                     /locus_tag="ACHUU3_001265"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR2081387.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            33306..33896
                     /gene="caiE"
                     /locus_tag="ACHUU3_001266"
     CDS             33306..33896
                     /gene="caiE"
                     /locus_tag="ACHUU3_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR2081388.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(34105..34500)
                     /gene="caiF"
                     /locus_tag="ACHUU3_001267"
     CDS             complement(34105..34500)
                     /gene="caiF"
                     /locus_tag="ACHUU3_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR2081389.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
BASE COUNT         8300 a         9354 c         9141 g         7820 t
ORIGIN      
        1 gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg
       61 gatcctacct gacgcttttt ttcgcaactc tctactgttt ctccataccc gtttttttgg
      121 atggagtgaa acgatggcga ttgcaattgg cctcgatttt ggcagtgatt ctgtgcgagc
      181 tttggcggtg gaatgcgcta ccggtgaaga gatcgccacc agcgtagagt ggtatccccg
      241 ttggcagaaa gggcaatttt gtgatgcccc gaataaccag ttccgccatc atccgcgtga
      301 ctacattgag tcaatggaag cggcgctgaa aatcgtgctt gcagagctta gcgccgaaca
      361 gcgcgcagct gtggtcggga ttggcgttga cacaaccggc tcgacgcccg caccgattga
      421 cgccgacggt aacgtgctgg cgctgcgccc agagtttgcc gaaaacccaa acgcgatgtt
      481 cgtattgtgg aaagaccaca ctgcggttga agaagcggaa gagattaccc gtttgtgcca
      541 cgcgccgggc aacgttgact actcccgcta tattggcggt atttattcca gcgaatggtt
      601 ctgggcaaaa atcctgcatg tgactcgcca ggacagcgcc gtggcgcaat ctgccgcatc
      661 gtggattgag ctgtgcgact gggtgccagc tctgctttcc ggtaccaccc gcccgcagga
      721 tattcgtcgc ggacgttgca gcgccgggca taaatctctg tggcacgaaa gctggggcgg
      781 cttgccgcca gccagtttct ttgatgagct ggacccgatc ctcaatcgcc atttgccttc
      841 cccgctgttc actgacacct ggactgccga tattccggtg ggcaccttat gcccggaatg
      901 ggcgcagcgt ctcggcctgc ctgaaagcgt ggtgatttcc ggcggcgcgt ttgactgcca
      961 tatgggcgca gttggcgcag gcgcacagcc taacgcactg gtaaaagtta tcggtacttc
     1021 cacctgcgac attctgattg ccgacaaaca gagcgttggc gagcgggcag ttaaaggtat
     1081 ttgcggtcag gttgatggca gcgtggtgcc tggatttatc ggtctggaag caggccaatc
     1141 ggcgtttggt gatatctacg cctggtttgg tcgcgtactc ggctggccgc tggaacagct
     1201 tgccgcccag catccggaac tgaaagcgca aatcaacgcc agccagaaac aactgcttcc
     1261 ggcgctgacc gaagcatggg ccaaaaatcc gtctctggat cacctgccgg tggtgctcga
     1321 ctggtttaac ggtcgtcgca cgccaaacgc taaccaacgt ctgaaagggg tgattaccga
     1381 tcttaacctc gctaccaacg ctccgctgct gttcggcggt ttgattgctg ccaccgcctt
     1441 tggcgcacgc gcaatcatgg agtgctttac cgatcagggg atcgccgtca ataacgtgat
     1501 ggcgctgggc ggcatcgcgc ggaaaaacca ggtcattatg caggcctgct gcgacgtgct
     1561 gaatcgcccg ctgcaaattg ttgcctctga ccagtgctgt gcgctcggtg cggcgatttt
     1621 tgctgccgtc gccgcgaaag tgcacgcaga catcccatca gcccagcaaa aaatggccag
     1681 tgcggtagag aaaaccctgc aaccgcgcag cgaacaggca caacgctttg aacagcttta
     1741 tcgccgctat cagcaatggg cgatgagcgc cgaacaacac tatcttccaa cttccgcccc
     1801 ggcacaggct gcccaggccg ttgcgactct ataaggacac gataatgacg atttttgata
     1861 attatgaagt gtggtttgtc attggcagcc agcatctgta tggcccggaa accctgcgtc
     1921 aggtcaccca acatgccgag cacgttgtta atgcgctgaa tacggaagcg aaactgccct
     1981 gcaaactggt gttgaaaccg ctgggcacca cgccggatga aatcaccgct atttgccgcg
     2041 acgcgaatta cgacgatcgt tgcgctggtc tggtggtgtg gctgcacacc ttctccccgg
     2101 ccaaaatgtg gatcaacggc ctgaccatgc tcaacaaacc gttgctgcaa ttccacaccc
     2161 agtttaacgc ggcgctgccg tgggacagta tcgatatgga ctttatgaac ctgaaccaga
     2221 ctgcacatgg cggtcgcgag ttcggcttca ttggcgcgcg tatgcgtcag caacatgccg
     2281 tggttaccgg tcactggcag gataaacaag cccatgagcg tatcggctcc tggatgcgcc
     2341 aggcggtctc taaacaggat acccgtcatc tgaaagtctg ccgttttggc gataacatgc
     2401 gtgaagtagc ggtcaccgat ggtgataaag ttgccgcaca gatcaagttc ggtttctccg
     2461 tcaatacctg ggcggttggc gatctggtgc aggtggtgaa ctccatcagc gacggcgatg
     2521 ttaacgcgct ggtcgatgag tacgaaagct gctacaccat gacgcctgcc acacaaatcc
     2581 acggcgaaaa acgacagaac gtgctggaag cggcgcgtat tgagctgggg atgaagcgtt
     2641 tcctggaaca aggtggcttc cacgcgttca ccaccacctt tgaagatttg cacggtctga
     2701 aacagcttcc aggtctggcc gtacagcgtc tgatgcagca gggttacggc tttgcgggcg
     2761 aaggcgactg gaaaaccgcc gccctgcttc gcatcatgaa ggtgatgtca accggtctgc
     2821 agggcggcac ctcctttatg gaggactaca cctatcactt cgagaaaggt aatgacctgg
     2881 tgctcggctc ccatatgctg gaagtctgcc cgtcgattgc cgtagaagag aaaccgatcc
     2941 tcgacgttca gcatctcggt attggtggta aggacgatcc tgcccgactg atcttcaata
     3001 cccaaaccgg tccagcgatt gtcgccagcc tgattgatct cggcgatcgt taccgtctgc
     3061 tggttaactg tatcgacacg gtgaaaacac cgcactccct gccgaaactg ccggtggcga
     3121 atgcgctgtg gaaagcgcaa ccggatctgc caactgcttc cgaagcgtgg atcctcgctg
     3181 gtggcgcgca ccataccgtc ttcagccatg cgctgaacct caacgatatg cgccagttcg
     3241 ccgagatgca cgacattgaa atcacggtga ttgataacga cacccgcctg ccagcgttta
     3301 aagacgcact gcgctggaac gaagtgtatt acggatttcg tcgctaagta gccacatcag
     3361 gtatgtaacg cctgatgcga cgctgacgcg tcttatctgg cctacacgct gcgattttgt
     3421 aggccggata agcaaagcgc atccggcatt gttttccccc tcgccccttt ggggagaggg
     3481 ccggggtgag gggaaacgac ctctcaggag tcaacatgtt agaagatctc aaacgccagg
     3541 tattagaagc caacctggcg ctgccaaaac acaacctggt cacgctcaca tggggcaacg
     3601 tcagcgccgt tgatcgcgag cgcggcgtct ttgtgatcaa accttccggc gtcgattaca
     3661 gcgtcatgac cgctgacgat atggtcgtgg ttagcatcga aaccggtgaa gtggttgaag
     3721 gtacgaaaaa gccctcctcc gacacgccaa ctcaccggct gctctatcag gcattcccct
     3781 ccattggcgg cattgtgcat acgcactcgc gccacgccac catctgggcg cagtcgggtc
     3841 agtcgattcc agcaaccggc accacccacg ccgactattt ctacggcacc attccctgca
     3901 cccgcaaaat gaccgacgca gaaatcaacg gcgaatatga gtgggaaacc ggtaacgtca
     3961 tcgtagaaac ctttgaaaaa cagggtatcg atgcagcgca aatgcccggc gttctggtcc
     4021 attcccacgg cccgtttgca tggggcaaaa atgccgaaga tgcggtgcat aacgccatcg
     4081 tgctggaaga ggtcgcttat atggggatat tctgccgtca gttagcgccg cagttaccgg
     4141 atatgcagca aacgctgctg gataaacact atctgcgtaa gcatggcgcg aaggcatatt
     4201 acgggcagta atgactgtat aaaaccacag ccaatcaaac gaaaccaggc tatactcaag
     4261 cctggttttt tgatggattt tcagcgtggc gcaggcaggt tttatcttaa cccgacactg
     4321 gcgggacacc ccgcaaggga cagaagtctc cttctggctg gcgacggaca acgggccgtt
     4381 gcaggttacg cttgcaccgc aagagtccgt ggcgtttatt cccgccgatc aggttccccg
     4441 cgctcagcat attttgcagg gtaaacaagg ctttcgcctg acaccgctgg cgttaaagga
     4501 ttttcaccgc cagccggtgt atggccttta ctgtcgcgcc catcgccaat tgatgaatta
     4561 cgaaaagcgc ctgcgtgaag gtggcgttac cgtctacgag gccgatgtgc gcccgccaga
     4621 acgctatctg atggagcggt ttatcacctc accggtgtgg gtcgagggtg atatgcacaa
     4681 tggcactatc gttaatgccc gtctgaaacc gcatcccgac tatcgtccgc cgctcaagtg
     4741 ggtttctata gatattgaaa ccacccgcca cggtgagctg tactgcatcg gcctggaagg
     4801 ctgcgggcag cgcatcgttt atatgctggg gccggagaat ggcgacgcct cctcgcttga
     4861 tttcgaactg gaatacgtcg ccagccgccc gcagttgctg gaaaaactca acgcctggtt
     4921 tgccaactac gatcctgatg tgatcatcgg ttggaacgtg gtgcagttcg atctgcgaat
     4981 gctgcaaaaa catgccgagc gttaccgtct tccgctgcgt cttgggcgcg ataatagcga
     5041 gctggagtgg cgcgagcacg gctttaaaaa cggcgtcttt tttgcccagg ctaaaggtcg
     5101 gctaattatc gacggtatcg aggcgctgaa atccgcgttc tggaatttct cttcattctc
     5161 gctggaaact gtcgctcagg agctattagg cgaaggaaaa tctatcgata acccgtggga
     5221 tcgaatggac gaaattgacc gccgtttcgc cgaagataaa cctgcgctgg caacttataa
     5281 cctgaaagat tgcgagctgg tgacgcagat cttccacaaa actgaaatca tgccattttt
     5341 actcgaacga gcaacggtga acggcctgcc ggtggatcgg cacggcggtt cggtggcagc
     5401 gtttggtcat ctctattttc cgcgaatgca tcgcgctggt tatgtcgcgc ctaatctcgg
     5461 cgaagtgccg ccgcacacca gccctggcgg ctacgtgatg gattcacggc cagggcttta
     5521 tgattcagtg ctggtgctgg actataaaag cctgtacccg tcgatcatcc gcacctttct
     5581 gattgatccc gtcgggctgg ttgaaggcat ggcgcagcct gatccagagc acagtaccga
     5641 aggttttctc gatgcctggt tctcacggga gaaacattgc ctgccggaga ttgtgactaa
     5701 catctggcac gggcgcgatg aagccaaacg tcagggtaat aaaccacttt cgcaggcgct
     5761 gaagatcatt atgaatgcct tttatggcgt gcttggcacc accgcctgcc gctttttcga
     5821 tccgcgcctg gcatcgtcga tcaccatgcg tggtcatcag atcatgcggc aaaccaaagc
     5881 attgattgaa gcgcagggct acgacgttat ctacggagat accgactcga cttttgtctg
     5941 gctaaaaggt gcacattcgg aagaagaggc ggcgaaaatc ggtcgtgcac tggtgcagca
     6001 cgttaacgcc tggtgggcgg aaacgctgca aaaacaacgg ctgaccagcg cattagaact
     6061 ggagtatgaa actcatttct gccgttttct tatgccaacc attcgcggag ctgataccgg
     6121 cagtaaaaag cgctacgccg ggctgattca ggagggcgat aaacagcgga tggtgtttaa
     6181 agggctggaa accgtgcgca ccgactggac gccgctggcc cagcagtttc agcaggagct
     6241 atacctgcgc atcttccgca acgagccata tcaggaatat gtacgcgaaa ccatcgacaa
     6301 actgatggcg ggtgaactgg atgcgcgact ggtttaccgt aaacgccttc gccgtccgct
     6361 gagcgagtat cagcgtaatg tgccgcctca tgtacgcgcc gctcgccttg ccgatgaaga
     6421 aaaccaaaag cgtggtcgcc ccttgcaata tcagaatcgc ggcaccatta agtacgtatg
     6481 gaccaccaac ggcccggagc cgctggacta ccaacgttca ccactggatt acgaacacta
     6541 tctgacccgc cagctacaac ccgtggcgga gggaatactc ccttttattg aggataattt
     6601 tgctacactt atgaccgggc aacttgggct attttgagca aaaaaagagt tcgccagata
     6661 ccattttgat gcgtgacgaa tgctttgcca tccagtacca tagcgccctt tccattcctg
     6721 gacctgaata acaccactac cccataagca cggtagcggg tggttattgc ctgcaattaa
     6781 agatatagag ccgaacacat atgcctttta cacttggtca acgctggatc agcgatacag
     6841 aaagcgaatt gggacttgga accgttgtcg cggtggatgc gcgaactgtc actttacttt
     6901 tcccatctac tggtgaaaac cgtctgtacg cacgcagtga ttcccccgtg acccgcgtga
     6961 tgttcaaccc tggtgatact attaccagcc atgacggctg gcagatgcaa gtcgaagaag
     7021 taaaagaaga aaatggcttg ctgacctata tcggtactcg cctggatact gaagagtccg
     7081 gcgtagccct gcgtgaagtt ttccttgata gcaaactggt gttcagcaaa ccgcaggacc
     7141 gtctgtttgc cgggcagatt gaccgtatgg accgctttgc gctgcgttat cgcgcgcgta
     7201 agtattccag cgaacagttc cgtatgccgt acagcggcct gcgcggtcag cgtaccagcc
     7261 tgatcccgca tcagctcaac atcgctcatg atgttggtcg ccgccacgcg ccgcgcgtcc
     7321 tgctggctga cgaagtgggt ttagggaaaa ccattgaagc cgggatgatc ctgcatcagc
     7381 aactgctctc tggcgctgct gaacgtgtgc taattatcgt cccggaaacc ttacagcatc
     7441 agtggctggt agaaatgctg cgccgtttca acctgcgctt tgcgctattt gatgatgagc
     7501 gttatgccga agctcagcac gatgcttaca acccgttcga caccgaacag ctggtgattt
     7561 gctcgctgga ttttgcccgt cgtagcaaac agcgcctgga acatctctgt gaagccgaat
     7621 gggacctgct ggtggtcgat gaagcgcatc acctggtgtg gagcgaagat gcgccaagcc
     7681 gtgaatatca ggccattgaa caactggcag agcacgtgcc gggcgttctg ctgctgaccg
     7741 cgaccccgga acagctgggg atggaaagcc acttcgcccg tctgcgtctg ctggacccga
     7801 accgtttcca cgattttgcg cagttcgttg aagagcagaa aaattatcgt ccggttgcgg
     7861 acgccgttgc catgctgctg gcaggtaaca aactgagcaa tgacgaactg aacatgctcg
     7921 gcgagatgat cggcgagcag gatatcgagc cgctgttgca ggcagcaaac agcgacagcg
     7981 aagatgccca gagcgcccgt caggagctgg tttcgatgct gatggatcgc cacggcacca
     8041 gccgcgtgct gttccgtaac acgcgtaacg gtgtgaaagg attcccgaaa cgcgagctgc
     8101 acaccattaa gctgccgctg ccgacgcagt atcagacggc tattaaagtc tccggcatta
     8161 tgggcgcacg taaaagtgcg gaagatcgtg ctcgcgatat gctctacccg gagcgtattt
     8221 atcaggaatt tgaaggtgat aacgccacct ggtggaactt cgatccgcgc gttgagtggc
     8281 tgatgggcta cctgaccagc catcgctctc agaaagtgct ggtgatctgc gccaaagctg
     8341 ccactgcgct gcaactggag caggtactgc gcgaacgtga aggtattcgc gctgcggtgt
     8401 tccacgaagg tatgtcgatt atcgaacgtg accgcgctgc cgcctggttt gccgaagaag
     8461 acaccggcgc acaggtactg ctgtgctcag aaatcggttc tgaaggacgt aacttccagt
     8521 tcgccagcca catggtgatg tttgacctgc cattcaaccc ggatctactg gagcagcgta
     8581 ttggtcgtct ggatcgtatc ggccaggcgc acgatattca gatccatgtg ccttatctgg
     8641 agaaaaccgc tcagtcggtg ctggtgcgct ggtatcacga aggtctggat gcatttgagc
     8701 acacctgccc gaccggacgc actatttacg atagcgtata caacgatctg attaactatc
     8761 tggcttcacc ggttcaaacc gaaggctttg acgatctgat caaaaactgc cgcgagcaac
     8821 atgaagcgct gaaagcacag ctggaacagg gtcgtgaccg cctgctggaa atccactcca
     8881 acggtggcga aaaagcccag gcactggcag aaagcattga agagcaggat gacgatacca
     8941 acctgatcgc cttcgccatg aacctgttcg atattatcgg tatcaatcag gacgatcgcg
     9001 gcgacaacat gatcgtgctg acgccgtccg atcatatgct ggtgccggac ttccctggcc
     9061 tgtcggaaga tggcatcacc atcacctttg atcgtgaagt ggcgctggcg cgtgaagatg
     9121 cacagtttat tacctgggag catccgctga tccgcaacgg tctggatctg atcctttctg
     9181 gcgatactgg tagcagcacg atttcactat tgaaaaacaa agcgttgccg gtaggtacgc
     9241 tgttggtgga actgatttat gtcgttgaag cccaggctcc gaagcagttg cagctcaacc
     9301 gcttcctgcc accgacgccg gtacgtatgc tgctggataa aaacggcaac aacctggcgg
     9361 cgcaggtaga gtttgaaacc tttaaccgtc agcttaacgc ggttaaccgt cacaccggca
     9421 gcaaactggt taacgccgtg cagcaggatg ttcacgctat ccttcagctg ggtgaagcgc
     9481 agatcgagaa atctgcccgt gcattgattg atgccgcacg taacgaagcc gacgaaaaac
     9541 tgtctgccga gctgtctcgt ctggaagcgc tgcgcgcagt gaacccgaac attcgtgacg
     9601 acgaactgac cgccattgag agcaaccgtc agcaggtaat ggaaagcctg gatcaggcag
     9661 gctggcgtct ggatgccctg cgtttgatcg ttgtaacgca tcagtaacgg agccgaaaat
     9721 ggggatggaa aactacaatc caccgcagga accctggttg gttatcctgt atcaggatga
     9781 ccacattatg gtggttaaca agccgagcgg tttgttgtct gtgccggggc gtctggaaga
     9841 gcacaaagac agcgtgatga cgcgcattca gcgtgattat ccgcaggcag aatcggtgca
     9901 tcgtctggat atggctacca gcggcgtgat tgtagtggcg ctgaccaaag ccgcggagcg
     9961 ggagttaaaa cgccagttcc gcgagcgcga gccgaaaaag cagtatgtag cccgcgtctg
    10021 ggggcatcca tcccctgcgg aaggtctggt ggatctgccg ctgatttgcg actggccaaa
    10081 ccgcccgaaa cagaaagtct gttacgaaac gggtaaacct gcgcagacgg aatatgaagt
    10141 ggtggagtat gcggcggata acacggcaag agtggtgtta aaaccgatta ccgggcgttc
    10201 gcatcagctg cgtgtgcata tgctggcgct gggtcatccg attctcggcg atcgttttta
    10261 tgcatcacca gaagcgagag cgatggcacc gcgtttgttg ctacatgcag agatgttgac
    10321 gattacccat ccggcgtatg gcaatagtat gacgtttaaa gcgccagcgg atttttaatc
    10381 gtcacgctgg cgcagttcgg taggcggata aggcgttcac gccgcatccg gcaattgtgc
    10441 gccgattgcc tgatgcgacg cgggccgcgt cttatcaggc ctacagttta aaacttattt
    10501 aaaccctttc tgctgcttta tcagctcata tgcctgctga atttcctgcg ctttctgctt
    10561 cgccatctcc atcatctcag gcggcaaacc tttcgccacc agcttatcgg gatggtgttc
    10621 actcatcagc ttacggtagg cacgtttgat ggtggtcgca tcatccgtcg gcttcacgcc
    10681 cagcacatta caggcatctt ccagcgtcgg gccacgctgc gcttgctgcc agttaccacc
    10741 gccagattgc tgctgataac cgccgccaaa ctgtgcaccg ccctgcatca tgcgcaaaaa
    10801 ctggtcaaac tgggcgcggg agatccctaa ttcttcagca atgacataca gcaccgcccg
    10861 ttcattcggg tgcagtgaac catcagcaaa cgccgcctga atctggatct ccagaaacat
    10921 acgaattaag tcaaaacgac caaagcagac actgcgaaac tggcgcatct tttcgcgcag
    10981 cgggtaatta tctgattttc ccacccggaa cgcgttttgc gccgcagtac gggaagcgcc
    11041 atgaaggttc attcggtcca tcaactggct ggcgatatga atatcagcct ccgtgacgcg
    11101 acctttggat ttggttaaat gccccatcac ttcaaaagtg gtggcaaaaa acagcgcctg
    11161 acgctcacgc tggttggcga accacgccat tttacggcta cgggctttat caaacatatg
    11221 gccaattaac aggcctaaca ctacgcccca aaagccgccg cccatcagta aggccacggc
    11281 cacgccaatg atttttcccc aatactgcat atattcccca aatcgacaca cggatatcag
    11341 ggctatctcc cacaatataa aggtgctttt accgttttcc ggctgcggtc aattgtggga
    11401 catcgcctat aatttgcatt atcatacctg tcattcaatg ccgtgcctaa caccactgac
    11461 gctattcgga caggattaac actagcgtag agatgacgag tacgttagtc tctgaccgtt
    11521 tgtcacgcgc aacgttaccg atgatggaac aataaaatca acgtatgaaa aaacgtatcc
    11581 ccactctcct ggccaccatg attgccaccg ccctttatag tcaacaggga ctggcagccg
    11641 acctcgcctc acagtgcatg ttgggcgtgc caagctatga ccgtcctctg gtacagggcg
    11701 ataccaatga cttacccgtg actatcaatg ctgaccacgc gaaaggggac tacccggatg
    11761 acgccgtgtt tactggcagc gtggatatca tgcagggtaa cagccgtctg caggccgacg
    11821 aagtgcagct ccatcaaaaa gaggcaccag gacaaccgga gccggtacgt accgttgatg
    11881 cgctcggtaa tgtccattac gacgataacc aggtgatcct caaagggccg aaaggctggg
    11941 cgaatctgaa caccaaagat accaacgtct gggaaggtga ttaccagatg gtgggtcgcc
    12001 agggtcgcgg taaagcggac ctgatgaaac aacgtggcga aaaccgctat accattctgg
    12061 ataacggtag ctttacctcc tgtctgccgg gttctgacac ctggagcgtg gtaggtagcg
    12121 aaattattca tgaccgcgaa gaacaagttg cggagatctg gaacgcccgc tttaaggtgg
    12181 gtccggtacc gatcttttat agcccctatt tgcagttgcc ggtgggtgac aaacgtcgct
    12241 ctggtttctt gatcccgaac gccaagtaca ccaccaccaa ctactttgag ttctacctgc
    12301 catattactg gaacatcgcg ccaaatatgg atgccaccat cacgccgcat tatatgcatc
    12361 gtcgtggcaa catcatgtgg gagaacgaat tccgctacct ctcccaggcg ggcgctggct
    12421 tgatggaact ggactatctg ccttcagata aagtctatga agatgaacac ccgaacgatg
    12481 acagttcacg tcgttggttg ttctactggc aacactccgg ggtcatggat caagtgtggc
    12541 gtttcaacgt cgactacacc aaggtcagcg atcctagcta cttcaatgat ttcgataaca
    12601 agtacggttc cagtactgac ggctacgcaa cgcaaaaatt cagcgttggc tatgcggtgc
    12661 aaaacttcaa tgccaccgtt tcaaccaagc agttccaggt ctttagcgag cagaacacca
    12721 gtagctactc ggcagagccg cagttagacg ttaactacta ccagaatgat gttggtccgt
    12781 ttgatacgcg tatttacggc caggcagtgc actttgttaa caccagagac gacatgcctg
    12841 aagcaacccg tgttcacctg gaaccgacca tcaatttgcc gctctctaat aactggggca
    12901 gcatcaatac cgaagcgaag ttgctggcaa cccattatca gcaaaccaat cttgactggt
    12961 ataactccag aaacacgacc aagctggacg aatccgttaa ccgcgttatg ccgcaattca
    13021 aagttgacgg caaaatggtc tttgaacgcg atatggaaat gctggctccg ggttataccc
    13081 aaacgctgga accgcgcgcg cagtatttgt acgtgccgta tcgcgatcag agcgacatct
    13141 ataactacga ctcgtctcta ctgcaatctg actactctgg cctgttccgg gaccggactt
    13201 acggcggtct tgaccgtatt gcctccgcta accaggtgac gaccggtgtc acatctcgca
    13261 tatatgatga tgctgccgtt gaacgtttta atatttccgt tggtcaaatc tactatttca
    13321 cggagtctcg cactggcgat gacaacataa catgggagaa tgacgacaaa acgggctcac
    13381 tggtgtgggc aggcgatact tactggcgta tctccgagcg ttggggattg cgtggcggga
    13441 ttcagtacga tacacgtctg gataacgtag cgaccagtaa ctccagcatt gaataccgtc
    13501 gggatgaaga ccgtctggta cagctgaatt accgttacgc cagcccggaa tatattcagg
    13561 ctacgctgcc taagtactat tccactgctg agcaatataa gaatggtatt tcgcaggtag
    13621 gtgctgtcgc cagctggcca attgccgatc gttggtccat tgttggggcc tactactacg
    13681 acaccaatgc gaacaagcaa gccgactcta tgttaggtgt gcaatacagc tcctgctgct
    13741 atgcaattcg cgtcggttac gagcggaagc tgaacggttg ggataacgat aaacaacatg
    13801 cggtatatga caacgcaatc ggctttaaca tcgaacttcg cggcctgagc tccaactacg
    13861 gtctgggtac gcaagagatg ctgcgttcga acattctgcc gtatcaaaac actttgtgat
    13921 ttgttgattt accacgtaat ccgcagtgcg gttaattgaa atggaaaaag tatgaagaac
    13981 tggaaaacgc tgcttctcgg tatcgccatg atcgcgaata ccagtttcgc tgccccccag
    14041 gtagtcgata aagtcgcagc cgtcgtcaat aacggcgtcg tgctggaaag cgacgttgat
    14101 ggattaatgc agtcggtaaa actgaacgct gcgcaggcaa ggcagcagct tcctgatgac
    14161 gcgacgctgc gccaccaaat catggaacgt ttgatcatgg atcaaatcat cctgcagatg
    14221 gggcagaaaa tgggagtgaa aatctccgat gagcagctgg atcaggcgat tgctaacatc
    14281 gcgaaacaga acaacatgac gctggatcag atgcgcagcc gtctggctta cgatggtctg
    14341 aactacaaca cctatcgtaa ccagatccgc aaagagatga ttatctctga agtgcgtaac
    14401 aacgaggtgc gtcgtcgcat caccatcctg ccgcaggaag tcgaatccct ggcgcagcag
    14461 gtgggtaacc aaaacgacgc cagcactgag ctgaacctga gccacatcct gatcccgctg
    14521 ccggaaaacc cgacttctga tcaggtgaac gaagcggaaa gccaggcgcg cgccattgtc
    14581 gatcaggcgc gtaacggcgc tgatttcggt aagctggcga ttgctcattc tgccgaccag
    14641 caggcgctga acggcggcca gatgggctgg ggccgtattc aggagttgcc gggtatcttc
    14701 gcccaggcat taagcaccgc gaagaaaggc gacattgttg gcccgattcg ttccggcgtt
    14761 ggcttccata ttctgaaagt taacgacctg cgcggcgaaa gcaaaaatat ctcggtgacc
    14821 gaagttcatg ctcgccatat tctgctgaaa ccgtcgccga tcatgactga cgaacaggcc
    14881 cgtgtgaaac tggaacagat tgctgccgat atcaagagtg gtaaaacgac ttttgctgcc
    14941 gcagcgaaag agttctctca ggatccaggc tctgctaacc agggcggtga tctcggctgg
    15001 gctacaccag atattttcga tccggccttc cgtgacgccc tgacccgcct gaacaaaggt
    15061 caaatgagtg caccggttca ctcttcattc ggctggcatt taatcgaact gctggatacc
    15121 cgtaatgtcg ataaaaccga cgctgcgcag aaggatcgtg cataccgcat gctgatgaac
    15181 cgtaagttct cggaagaagc agcaagctgg atgcaggaac aacgcgccag cgcctacgtt
    15241 aaaatcctga gcaactaatg gttaaaaccc aacgtgttgt gatcactccc ggcgagcccg
    15301 ccgggattgg cccggactta gttgtccagc ttgcacagcg tgagtggccg gtcgaactgg
    15361 ttgtttgtgc cgatgccact ctccttaccg accgggcagc gatgctcggt ttgccgctca
    15421 ccctccgccc ttattccccc aactcccctg cacaaccgca aactgcgggc acattaacgc
    15481 tacttcctgt cgcgctacgc gaatctgtca ctgcggggca gttagcgatt gaaaatgggc
    15541 attacgtggt ggagacgctg gcgcgagcgt gtgatggctg tctgaacggt gaatttgctg
    15601 cgctgatcac aggccccgtg cataaaggcg tcattaacga cgcaggtatt ccgtttactg
    15661 gtcataccga gtttttcgaa gagcgctcgc aggcgaaaaa agtggtgatg atgctggcga
    15721 ccgaagaact tcgcgtggcg ctggcaacga cgcatttacc gctgcgcgat atcgcagatg
    15781 ctatcacccc tgcacttttg cacgaagtga ttgctatttt gcatcacgat ttgcgtacaa
    15841 aatttggtat tgccgaaccg cgcattctgg tctgcgggct gaatccgcac gcgggcgaag
    15901 gcggtcatat gggtacggaa gagatagaca ccattattcc ggtgctcaac gagctgcggg
    15961 cgcaggggat gaaactcaac gggccgctgc ctgccgatac cctgtttcag ccgaaatatc
    16021 tcgataacgc cgacgccgtg ctggcgatgt accacgatca gggtcttccc gtgctaaaat
    16081 accagggctt cgggcgcggt gtgaacatta cgctgggcct gccctttatt cgcacatcag
    16141 tggaccacgg caccgcgctt gaactggcgg gacgtggcga agccgatgtc ggcagtttta
    16201 ttacggcgct taatctcgcc atcaaaatga ttgttaacac ccaatgaata atcgagtcca
    16261 ccagggccac ttagcccgta aacgcttcgg gcaaaacttt ctcaacgatc agttcgtgat
    16321 cgacagcatt gtttctgcca ttaacccgca aaagggccag gcaatggtcg aaatcggccc
    16381 cggtctggcg gcattgaccg agccggtcgg cgaacgtctg gaccagctga cggtcatcga
    16441 acttgaccgc gatctggcgg cacgtctgca aacgcatcca ttcttaggcc cgaaactgac
    16501 gatttatcag caggatgcga tgacctttaa ctttggcgaa ctggccgaga aaatgggtca
    16561 gccgctgcgt gttttcggca acctgcctta taacatctcc acgccgttga tgttccatct
    16621 gtttagctat actgatgcca ttgccgacat gcactttatg ttgcaaaaag aggtggtgaa
    16681 tcgtctggtt gcaggaccga acagcaaagc gtatggtcga ttaagcgtca tggcgcaata
    16741 ctattgcaac gtgatcccag tgctggaagt accgccgtca gcctttacac caccacccaa
    16801 agtggattcc gccgtcgtgc gcctggttcc tcatgcaacg atgcctcacc cggttaaaga
    16861 tgttcgcgtg ttgagccgca tcaccactga agcctttaac cagcgtcgta aaaccattcg
    16921 taacagcctc ggcaacctgt ttagcgtcga ggtgttaacg ggaatgggga tcgacccggc
    16981 gatgcgagcg gaaaatatct ctgtcgcgca atattgccag atggcgaact atctggcgga
    17041 gaacgcgcct ttgcaggaga gttaacgatg atcaattcgc cccgagtgtg tattcaggtt
    17101 caaagcgtct acattgaggc acaatcttca cctgataatg aacgttacgt ttttgcttat
    17161 accgtaacca tacgcaatct ggggcgagcg ccagtgcagt tgttggggcg ttactggctg
    17221 atcaccaatg gcaatggccg tgaaaccgaa gtccagggcg aaggagtggt tggcgtccag
    17281 ccacttatcg cgcctggcga agagtaccag tacaccagcg gtgcaatcat tgaaaccccg
    17341 ctgggcacca tgcagggtca ctacgaaatg atcgatgaaa atggcgtccc tttcagcatc
    17401 gacattcccg tattccgact cgccgttccc acactcattc attaaaagaa tatggcgaca
    17461 taccttattg gcgacgttca tggttgttac gatgaactga tcgcattgct gcataaagta
    17521 gaatttaccc ctgggaaaga taccctctgg ctgacgggcg atctggtcgc gcgcgggccg
    17581 ggttcgctgg atgttctgcg ctatgtgaaa tccttaggcg acagcgtacg tctggtgctg
    17641 ggcaatcacg atctgcatct gctggcggta tttgccggga tcagccgcaa taaaccgaaa
    17701 gatcgcctga caccgctgct ggaagcgccg gatgccgacg agctgcttaa ctggctgcgt
    17761 cgccagcctt tgctgcaaat cgacgaagag aaaaagttgg tgatggccca cgcagggatc
    17821 acgccgcagt gggatctaca gaccgccaaa gagtgcgcgc gcgatgtaga agcggtgcta
    17881 tcgagtgact cctatccctt ctttctcgac gctatgtacg gcgatatgcc aaataactgg
    17941 tcaccggaat tgcgggggct gggaagactg cgttttatca ccaacgcttt tacccgtatg
    18001 cgtttttgct tcccgaacgg tcaactggat atgtacagca aagaatcgcc ggaagaggcc
    18061 cctgccccac tgaaaccgtg gtttgcgatt cctggccctg tcgctgaaga atacagcatc
    18121 gcctttggtc actgggcatc gctggagggc aaaggtacgc cggaaggtat atacgcgctg
    18181 gataccggct gctgctgggg tggtacatta acctgcctgc gctgggaaga taaacagtat
    18241 tttgtccagc cgtcgaaccg gcataaggat ttgggcgaag cggcggcgtc ttaaacacag
    18301 cctgatatag gaaggccgga taagacgcga ccggcgtcgc atccggcact agccgtaaat
    18361 tctatacaaa attaccgccg ctccagaatc tcaaagcaat agctgtgaga gttctgcgca
    18421 tcagcatcgt ggaattcgct gaataccgat tcccagtcat ccggctcgta atccgggaaa
    18481 tgggtgtcgc cttccacttc tgcgtcgata tgcgtcagat acagtttctg cgcttttggc
    18541 aggaactgct cataaacgcg accgccgcca atcaccatga tttctggtac gtcaccacac
    18601 gccgcgatgg cttcatccac cgacttcacc cacgttacgc gatcgtccgt acccggttga
    18661 ctgctgagga taatattttt gcgtcctggc aacggacgac cgattgattc ccaagtatgg
    18721 cggcccataa tcacgggttt atttaaggtg ttgcgtttaa accaggcgag atcggcaggc
    18781 aggttccacg gcatggcgtt ttccatgccg ataacgcgat ctaccgctaa cgccgcaatc
    18841 agactgatca ttgagatttc ccgataaaaa aaattgtcgc cactatacgt aaagcgtaaa
    18901 ccgtcgtcga ctggtgcgag gatgatgttg aggaaaattt tatattctgc tggcgagtcc
    18961 acgctctctc cctggactcg ccgcattaca atgaaacaaa aacaaacagt tagctgtaaa
    19021 gtgtgattta cgtcactctt tattaggatg agggtttcgt ttccggttca tccgccatgt
    19081 tgccggtatg tttaccttct tcggttccct gccagccatg tcgttgaatt aatgacagat
    19141 gttcgcggtc ctcggtaatg atctcactta acatcgcgct ggtgcgttta tagaccgccg
    19201 cgcgggcttt ggtgtcgttc tcaaccattg ccatctcttc caccatctga atattaaagc
    19261 ggcggaacac atcggcacgt tctcgcgctt catacggccc cagacctaaa ctttccagtg
    19321 ccagacgccc ggttttcagc gcaccttcga aggtttcacg ctccggcttt tcaacgcctg
    19381 cctgacgcaa acgaatgtag tggtcgacat cgcgggcgcg ggcaataatc tgcaaatgcg
    19441 ggaagtgttc tttcaccatc tctgtcagtt gcaggttggt ttgcggatcg tcgatggcgt
    19501 taatcagcac ttccgctttc gccgctcccg cagattccag taaatccatc cgcgtggcat
    19561 cgccataaaa cactttcata ccaaatttac gcaaggtttc gatatggtcc ggatcgtgat
    19621 cgagcaccac cattttcacc ccgctggaga gcagtaaacg tccggtgatc tgcccaaaac
    19681 gaccgaatcc ggcaatgatt acccgcggct gttcttcgtc gatctcatca gcttcacgcg
    19741 cctcttcagt agaagattgc tcaaggcgat tgaggatcac cagcagaatc ggcgttgccg
    19801 ccatcgacag cgccaccgcc agggtcagcg atttcgccca ctccggttcc agcacattcg
    19861 ccatctgcgc cgtgccaaat accacaaagg caaactcact gccctgccct aacaacaccg
    19921 caaaccaacg acgctgttta tttggcactt gcaacggtcg ggcaatcagc cacagcatgg
    19981 cgattttgat gatgaggaaa ccgagcagca aaatgacaat gcgcaatggg ttttcaagca
    20041 gcgtgccaaa gtctatcgac atgccaacac cgatgaaaaa cagccccaac agcaaacctt
    20101 taaatggttc gatatcgctc tccagcgcat gacggtattc cgagcttgcc agcagtacgc
    20161 ccgccagaaa cgcgcccatc gccatcgaca agccgacctc ttccagcagc aaaccaaagc
    20221 caaacacgag gaataacgcc acggcactaa acacttcccg caagccagag cgggctacaa
    20281 aacgcagcgc cggacgcgtg acatagcgcc ccagcaatac caccagcacc agcgcacccg
    20341 ccacttttaa cgccgagaga gcaaatgcgc ccatcgtcgt cgaggcactg ctcgttgcca
    20401 gtagcggaat catcgccacc agcgggatcg ccgcgatatc ctggaacagc agcaccgcaa
    20461 aggcactgcg acccatttgc gtcaccatca gattgcgttc attcatcgcc tgcatggcaa
    20521 tcgccgtaga ggagagcgcc agcgtcatgc cgatcaattc cgcgacctgc cagtgcagcc
    20581 caagtaacat gcagaacagc cccagcaggc cgccgcaaat caccatctgc aatgcgccac
    20641 cgccgaacac tgccgcacgc agcttccaca gcctttgtgg atcgagttcg aggccgataa
    20701 taaacagcat cagcaccacc ccaatctcgg caaagtgcag aatagattcg gcatcggtca
    20761 ccagtcgcag cccccacggg ccaataatgc agccggcgat caggtagcca agtaccgatc
    20821 ccagaccaag acgtaccgca atgggtacaa tcagcgctgc cgaaccgaga taaatcagcg
    20881 cctgaatcag cgtatggcta tccatgatgg gcctcctgcc attccagcag acgttgctta
    20941 tagtgacgcg cctgcccttc gagggtttcg tcgtcacaaa taaaggtgca gtgcatggca
    21001 aacggtggca gccagttcag cccgcagtag attgccgtcg cctgtagcgg ctgcgacagc
    21061 acatcaaagc ccggatgcgc accaatttca aaatggcttt ccccgccgcc ggtcgtcacc
    21121 gcccacagca aatatttgcc atgcagcgcc ttgccgccgt gaccgtaagc ccagccgtgc
    21181 gacaaaactt tatcgatcca aagtttaaga agcggaggaa tgctgtacca ctgcatcgga
    21241 tgctgccaga cgatcagatc ggcgcgagac agcgcctcct gctcggcggc aatatcgata
    21301 ttgaagtcag gatagagttg ataaagagag cgaatttcga cgccttccag cgtccttgcc
    21361 tgttcaagca tccgtttatt cgcatgggaa tgatgcggat acggatgcgc ataaattata
    21421 agaatcatag gtagcccgcg tgattacgcc tttgttttcc cacaaagtgt agtcatatat
    21481 tcacgcggct tatagtcaat aatattgatt cagaaaagag agaaatctga acaatttacc
    21541 ccattctctg cccgcggatc gtcatattgc tggtttgcgc cagtgacatc cctcgagtct
    21601 ccggggcaaa cgctacggaa atcaacaagc caaacagcga gatacccgcc tccattagca
    21661 tcgtgttact gataccgtaa ttattgataa agatcggcag tgcccaggtc gaaacaatgg
    21721 tgccaatacg gcttaaggac ataatcacgc ccacggcaga ggcgcggata tctgtcggga
    21781 agagttcatt aggatagagc cactgcaaat tacccggccc gccagagaaa aaggcataca
    21841 ccgcaaaggc catcactacc agccagatcc ccatatccgg gatcagcccc aaaaccgcca
    21901 gcgccagcgt catcatggca aagctgccaa tcaacaatgg acgccgtccg gcagtgttta
    21961 accacagcat cggcggaata cagccgagca taaagaacag gctaatcacc acgttcccca
    22021 gcgccgcgtt tttgccaaca cccaatccca acaaaccaac gatttgcggg ccaaaggtgt
    22081 aaatggcgaa cattgggatc acctggcagg tccagatggc ggcaacaaac agaacaaaag
    22141 gaaaatggcg gcgattaaac agatcgcgaa aacgggtttg ctacggctgc tcttcatcga
    22201 aagccaccgg ttcgccaaac agttttatca tcatctcttc gcactctttt actcgccctt
    22261 tgcgtaataa ccagcgggga gattcaggca gttcgaatcg accaatcaaa atcaacaaac
    22321 aggggatcgc cgcgctaccc agcatccagc gccagccgcc ttccacatca taaagccagt
    22381 agccgaccag atcggcgcag gtcgcgccga cataccacat ggcggcgata aagctgatgg
    22441 aaaatgcccg ctgacgggta ctggagaact cggtgatcat tgaggtggcg atgggataat
    22501 ctgcaccgat gacaatgccg ataagtaccc gcatcaccaa cagttcgacg ggggatgaaa
    22561 caaacatcgt cgccaccgat atcacgccga tggcgatgat atcaatgagg aacattttgc
    22621 gccgtcctac tttatcggaa atatagccaa acagcgatgt gccaacgaac agcccggcga
    22681 gcgttcccgc gcccaacaag ccaatccagt cagcgtccag ttttagcgcc ggagtcagtt
    22741 gctccagcgc cacgccaatc attaccagta cataaccatc cagaaacgga ccaccgcttc
    22801 cccacagcaa aatgcggcgg tgaattgagg agaatttgag atcgtcaaag tttctggacg
    22861 gttgcatggg ttattcctgt ttttttgcaa tgagagtcag tacggcgtta cgtatcaaac
    22921 caacatcagc cgtaacggaa ctccacacca aaggtgccgc gcgggtattc ccactgttcc
    22981 agcgccgacc ccagccccag aatgcgacag gtgccgcact ccagacatcc ggcgtaatcg
    23041 aagcgcacac tgccgtcatc ctgcttcttg tacagacctg cggggcacgc tttcaccagc
    23101 agctccaggg cctgtttatc ggcatcggcc ttcacaacaa tgtgcggatg ctcttcatcg
    23161 acattgaatt tattgacgcc cagtttgacg tccacattga cgggagaagt cataaaacgg
    23221 tcactccttt catgccatcc ttgatcagat tgatgaagcc cactttcttg ccgtggcgga
    23281 ggattttctt gcgcatcagt tccggcgcac tgccgtcaat ggtgaacagg tcacgcgcca
    23341 cgcccaccgc cagttccggg tagccgctaa acatgcgtgg gttatcaagg aaggccggta
    23401 gtttctggta catacgcata tcgcgcagcg ggccactctc aagatgctga cgatattccg
    23461 ccagtttttg cttactgaaa tcgtcgcttt tcatcgctga aagcacggtt tttgctgcgg
    23521 cttccccggc ggcaatcgcc agatccatac cgcgaatggt aaaaccgagg ttcatacaca
    23581 ttccggcggc atcaccggca atcaatacgc cgtcaccaac caactccggc agcatgttga
    23641 tgcctgcttc cggcactacg tgagcggaat attccaccag cttgccgccc gcgatcagcg
    23701 gtgccactgc cggatgctgt ttgaaatctt ccagcatttg cggcaccgat ttttttgcgt
    23761 catgcagatg atgcagacca caaaccagcc ccagcgacag ggtattttca ttggtataaa
    23821 ggaagccgcc gcccatcagg ccatcggtgg gtgatcccgc aaacagacaa gccgcgccct
    23881 gattaccctg caactgaaaa cggtcttcga ttaccgactt cggtaactcg atcagttcct
    23941 tcacgccaac cgccacatcc gtcggtttaa cgcgttttgc catccccagc ttttcggcaa
    24001 ggatggagtt cactccatca gcaaggatca ccgttttcgc ttcaatcaca tcgccatcgg
    24061 cttctacacc gacgactttg ccatcgcgct gtacgaggtt atcgacgcgg atcccggtaa
    24121 ttaactgcgc gcccgcttct tcggcctgct ccatcagcca ggcatcaaat ttactgcgca
    24181 aaacggagta agaacgctgg gatggcgagg tttcgtcacc gttgcagtag tccatagtca
    24241 tcgctgactt ttccgtcata aacgcgagtt tttcatgggt gatcaggcgt tctaccgggg
    24301 cggattcggc gaaaccagga ataatgtgtt ccaggctgtg ggcatagaga cgcccgccgg
    24361 tgacgttctt ggcacctgcg gaattgccac gctcgataac taacacttgc gccccttcgc
    24421 gggcgagcac cagtgcggca accgaaccgg caagcccagc accgacgatg atggcgtcaa
    24481 agatatcttc ggacataaga gctccctgtc agcggcaggg ccaaaatgca gccctgccag
    24541 agtggatcaa cgcgctaaag ctgcggtcag cgccggaagg atcttcacgg cgtcaccaac
    24601 aatgccgtaa tccgcgtact ggaagatcgg cgcattttta tctttattga tggcgaaaat
    24661 ggtttgcgat gcattagcac caaccatgtg ctggatctgc ccggagatcc ccaccgccag
    24721 gtacagttca ggtttcagca tcaggttgga gataccgaca tagcgttcgt gctccatcca
    24781 tttttcgttt tccgccaccg gacgagaaca ggccaactcc gcacctatcg ccttgcaaag
    24841 ctgttctgcc agcgcaatgt tctctttgct gccaataccg cgaccgacgc tgaccaccag
    24901 acgggctttg tcgagatcga cgctgttgct ctggcgcgcc tgggttgccg tgcgggtaat
    24961 cgccacagcc ggagcctgcc actccacggt gtgcgtttcg ccagtgcgtg acgcgtctgg
    25021 ctgagccgca tcgaacgtgc cgctgctgat ggtcagtacc gcatacggcg tggcaatgcg
    25081 ttcttcgcca atcgccagac caccgtaaac catatgtttc actgttgctt taccgtcctg
    25141 tacgctgacg gtgctggcat cgttagacac cgccgcttta aggcgataac ccagttttgc
    25201 cgccagtaat ttgccgcgac gggtgtttgg cagcagcacc aggccgtctg cgccgtgctg
    25261 gcgaatagtg tcagccatga caccggcgta atcttcgatc atccgatcgt ccggtttgcc
    25321 gtttaatttc cagacatgat tagcgccgag ctggattgcc tgtgcgccgt cggcatcatt
    25381 gaggacaaag gtgttgattt gattagctaa agcctgcgca ccgttcatca gttccggcag
    25441 acgagaaggg gtatcgctga atacccagac ttgagaaaac gtgttcatag catcccctgt
    25501 aattaaatga ctttgcgaag attttcagca aatgcggcga tctgttcttc gccgtcgcct
    25561 tcaatcacga tgcgctgacg ttcgcgctgt ttcggcgcgg caacctgttg ttctgaccag
    25621 gctgcctctg cgttaaaacc aatatccgcc gccgaccata cctggacggg ctttttcgcc
    25681 gcgccgagaa tggctttcat cgaaggaatt tgtggggaat tgatatcagt ggaaacagca
    25741 acaaccgcag gcagcggaat gcttaaggtt tcggtttcat cttccagttc gcgctcaacg
    25801 gtgagggtat ctgccgtcag ggagataatt ttgctgacgc cgttaactgc cggaatattg
    25861 aggatttcgc ccaccagcag accaacctgc tgggcataaa ggtcggaaga accatcgcca
    25921 cagaggatca aatcaaagcc tgctttctgg gcggctgcag ccagtgcgct cgccgtttgt
    25981 tgcggcagtg cctgctcgaa ctggtcatca atcaccacaa tcagttcatc cgggccgcgc
    26041 gatagtacat ctttacgccc tttggcgttg gtcagggctt taccgcccac acttaaggcc
    26101 gtcacctgcg cctctgctgc ctgttgcttt agctggcaag ccgcttcaat agcgttcaga
    26161 tcgtattggc ttattttggc atcggctttg ctgaagtcta atgaaccatc agcattattg
    26221 accgcaatat cctgttcatc aggcacgcac ttatagcaag taataatctt cattgcatct
    26281 ccagaaatca tgaaggaaac gtcgttcata tcggtggtgt taattacaga tatcacgccc
    26341 tttaatttgg aaataaaact ttaatcacca atattgaaaa tgtcacacgc agataaatcc
    26401 aactttcaat attgttaagt tcctcaccaa tattgaaaac atggcgtaga aaaaagaaat
    26461 tttcaatatt gctttatgga tcaccaatat tgaaagtcag atgctatttt tttactcaca
    26521 acacaacata acaaactgat taataatcaa aattaacgag aaaagcgccc tctattcacc
    26581 cgaaaaaaaa tgtgataaca atcacagaat gcagcttatt gaatacccat tatgagttag
    26641 ccattaacgc gtccacgagg ttaataataa ttatatcaaa tgttaacaaa aataaaacaa
    26701 acgggaacgc aaaataaata ttcgttttca cagtggaatt aactcatgaa gaatgaaaag
    26761 agaaaaacgg gaatagaacc gaaggttttc tttccgccgt taataatcgt cggcatactt
    26821 tgttggctta cagtcagaga tctggatgca gcgaatgtcg ttattaatgc tgtattcagt
    26881 tacgtcacca atgtatgggg atgggcattt gaatggtata tggtggtgat gcttttcggt
    26941 tggttctggc tggtgtttgg cccgtatgcc aaaaagcgtt taggtaacga accacctgaa
    27001 tttagtaccg ccagttggat ctttatgatg ttcgcctcct gtacgtctgc tgccgtactg
    27061 ttctggggat cgattgagat ctactactac atctccaccc cgccgtttgg cttagaaccg
    27121 aactcgacag gggcgaaaga gttggggctg gcttacagct tgttccactg gggacctctg
    27181 ccgtgggcca cttacagctt cctttcagtc gccttcgctt acttcttctt tgtccgcaaa
    27241 atggaagtga ttcgccccag ctcgacactg gtgccgctgg taggtgaaaa acacgccaaa
    27301 gggttgttcg gcactatcgt cgacaacttc tatctcgtcg ccttgatctt cgcgatgggt
    27361 accagtctgg gccttgccac gccgctggtg accgagtgta tgcaatggtt gtttggcatt
    27421 ccgcataccc tgcaactgga cgctatcatc attacctgct ggattatcct caacgccatt
    27481 tgcgtcgctt gcggtctgca aaaaggggta cgtatcgcca gtgacgtgcg tagttacctg
    27541 agcttcctga tgctgggttg ggtgttcatt gtcagcggtg ccagcttcat catgaactac
    27601 ttcaccgatt cggtggggat gttgctgatg tatctgccgc gcatgttgtt ctataccgat
    27661 cccatcgcta aaggcggctt cccgcagggc tggaccgtgt tctactgggc atggtgggtg
    27721 atttatgcta tccagatgag tatcttcctc gcccgcatct cccgtggtcg tactgtgcgt
    27781 gaactgtgct tcggcatggt gctggggctg acagcgtcaa cctggatcct gtggactgta
    27841 ctcggtagta acactctgct gttgatagat aaaaacatca tcaacattcc aaatctgatc
    27901 gaacagtacg gtgtggcgcg cgccatcatt gaaacctggg ccgctctgcc actcagcacc
    27961 gccaccatgt ggggcttctt catcctctgc tttattgcca ccgttacgct ggttaacgcc
    28021 tgctcttata ccctggcgat gtccacttgc cgcgaagtac gcgatggtga agaaccacct
    28081 ctgctggtgc gtatcggttg gtcaattctg gttggcatta tcggtattgt tctgctggcg
    28141 ctcggcggcc tgaaaccgat tcaaaccgcc attatcgccg gaggatgccc gctgttcttc
    28201 gtcaacatta tggtgacgct ctcctttatt aaagacgcga aacagaactg gaaagattaa
    28261 ttaaccccca aaatatcaag aggttgaaag atggatttta atttaaatga tgagcaggaa
    28321 ctgtttgtcg ccggtatccg cgaactgatg gccagcgaaa actgggaggc ctattttgcc
    28381 gagtgcgacc gtgacagcgt ctacccggaa cgttttgtca aagcactggc ggatatgggt
    28441 atcgacagtc tgctgatccc tgaagagcac ggtggtctgg acgcggggtt tgttactctc
    28501 gccgccgtgt ggatggagct gggacgtctg ggggcaccaa cctatgtgct gtaccagttg
    28561 ccgggcgggt tcaacacctt cctgcgcgaa ggcacacaag agcagatcga caaaattatg
    28621 gctttccgcg gcaccggtaa gcagatgtgg aactcagcga ttaccgaacc gggcgcgggc
    28681 tccgacgtgg gtagcctgaa aacgacttat acccgtagaa atggtaagat ttatcttaat
    28741 ggtagtaagt gttttattac cagcagcgcc tacaccccgt acatcgtggt gatggcgcgc
    28801 gacggggctt ctccggacaa acctgtctac accgaatggt ttgttgatat gagcaaaccg
    28861 ggcatcaaag tgaccaaact tgaaaagctc ggtctgcgta tggatagctg ctgtgaaatc
    28921 acctttgacg acgtggaact ggacgagaaa gacatgttcg gtcgggaagg taacggcttt
    28981 aaccgcgtca aagaagagtt cgaccatgaa cgtttcctgg tagccctcac caactacggt
    29041 acggcgatgt gcgcttttga agatgcggcg cgctacgcca atcagcgcgt gcagtttggc
    29101 gaggctattg gtcgtttcca gttgattcag gaaaaattcg cccacatggc gatcaaatta
    29161 aactccatga aaaacatgct gtatgaagca gcgtggaaag cagacaacgg caccatcacc
    29221 tctggcgatg cagcgatgtg caaatacttc tgcgccaatg cggcatttga agttgtggat
    29281 agcgcaatgc aggtgctggg cggtgtcggg attgcgggca accaccgcat cagccgcttc
    29341 tggcgtgatc tgcgtgtaga ccgcgtctcc gggggatctg acgaaatgca gatcctgacg
    29401 ctgggtcgcg cggtgctgaa gcaataccgc taagtccgtg cgctgcctga tgcgacgcta
    29461 atgcgtctta tcaggcctac gggagcacga atgtaggccg gataaagcgt ttacgccgca
    29521 tccggcagtc atgcgctaca taaatttttc aggagctaat aatggatcat ctacccatgc
    29581 cgaaattcgg gccgttggcc ggattgcgcg ttgtcttctc cggtatcgaa atcgccgggc
    29641 cgttcgccgg gcaaatgttc gcagaatggg gtgcggaagt tatctggatc gagaacgtcg
    29701 cctaggccga caccattcgc gttcaaccga actacccgca gctctcccgc cgcaatttgc
    29761 acgcgctgtc gttaaatatt ttcaaagatg aaggccgcga agcgtttctg aaataaatgg
    29821 aaaccaccga tatcttcatc gaagccagca aaggtccggc ctttgcccgt cgtggcatta
    29881 ccgatgaagt actgtggcag cacaacccga aactggttat cgctcacctg tccggttttg
    29941 gtcagtacgg caccgaggag tacaccaatc ttccggccta taacaccatc gcccaggcct
    30001 tcagaggtta cctgattcag aacggtgatg ttgaccagcc aatgcctgcc ttcccgtata
    30061 ccgccgatta cttttctggc ctgaccgcca ccacggcggc gctggcagca ctgcataaag
    30121 tgcgtgaaac cggtaaaggc gaaagtatcg acatcgccat gtatgaagtg atgctgcgta
    30181 tgggccagta cttcatgatg gattacttca acggcggcga aatgtgcccg cgcatgagca
    30241 aaggtaaaga tccctactac gccggttgcg gtctgtataa atgcgccgac ggctacatcg
    30301 tgatggaact ggtgggcatt acccaaattg aagagtgctt taaagatatt ggcctcgcac
    30361 atctactcgg tacgccggaa atcccggaag gcactcagct tatccaccgt atcgaatgcc
    30421 cttacggccc actggtagaa gaaaaactcg atgcctggct ggcggcacat accatcgcag
    30481 aagttaaaga acgctttgcc gaactgaata tcgcctgtgc caaagtactg accgtaccgg
    30541 aactggaaag caatccacag tatgttgccc gtgaatcaat cactcagtgg caaacgatgg
    30601 atggtcgcac ctgcaaaggg ccgaacatca tgccgaaatt caaaaataac cccggacaaa
    30661 tctggcgcgg aatgccctca catggcatgg acacggctgc cattttgaaa aatatcggct
    30721 acagcgaaaa cgacattcag gagttggtca gcaaaggtct ggccaaagtt gaggactaaa
    30781 cgcgtggcgt cagtcggctt gcctgactag cgcccgccca ataacaaacg aacaaaaatg
    30841 gatagaggtg caatggatat cattggcgga caacatctac gtcaaatgtg ggacgatctg
    30901 gcggacgttt acggtcataa aacggcgctg atttgtgaat ccagcggcgg agtcgttaac
    30961 cggtatagtt atcttgagtt aaatcaggag attaaccgca cgacaaacct gttttatacg
    31021 ctggggattc gcaaaggcga caaggttgct ctacatctcg acaactgccc ggaatttatc
    31081 ttttgctggt tcgggctggc aaaaattggc gcgattatgg tgccgattaa cgcccgcctg
    31141 ttgcgcgagg aaagcgcgtg gatcctgcaa aatagccagg cgtgcctgct ggtgaccagt
    31201 gcgcaattct atcctatgta tcaacagatt cagcaggaag atgccactca attgcggcac
    31261 atttgcctga cagatgtggc acttcccgct gatgatggcg tgagttcgtt tactcaactg
    31321 aaaaatcaac aacctgccac cttgtgctat gcaccgccgc tatcgactga cgatacggcg
    31381 gaaattctct tcacctccgg caccacctcc cgaccgaaag gtgtggtgat tacccattac
    31441 aacctgcgct tcgctggata ttactccgcc tggcagtgtg cactgcgtga cgatgacgtc
    31501 tacctgacgg taatgcctgc gtttcatatc gattgccagt gtactgcggc gatggcggcg
    31561 ttttctgccg gggccacctt tgtgctggtc gagaaataca gcgcccgcgc cttctgggga
    31621 caggtacaga agtaccgcgc caccgttacc gaatgtattc cgatgatgat ccgtaccttg
    31681 atggcgcagc cgccttcagc gaacgatcgg caacaccgcc tgcgggaagt gatgttttat
    31741 ctcaacttgt cggagcagga aaaagatgcg ttttgtgaac gcttcggcgt tcgcttgctg
    31801 acgtcttatg ggatgacgga aaccattgtg ggcattatcg gcgatcgccc tggcgataaa
    31861 cgacgctggc cgtcgattgg tcgggcgggg ttttgctacg aagcggagat ccgcgacgat
    31921 cacaatcgcc cgctcccggc aggtgagatc ggtgaaatct gtattaaagg cgtaccaggg
    31981 aaaaccatct tcaaagagta ttttctcaac ccgaaagcca ctgcgaaagt gctggaagcc
    32041 gacggctggc tacataccgg cgataccgga tactgcgacg aagagggctt tttttatttc
    32101 gtcgatcgcc gctgcaatat gatcaaacgc ggtggcgaga atgtctcctg cgtggagctg
    32161 gaaaatatca tcgccaccca tccaaaaatt caggatatcg tggttgtggg tattaaagat
    32221 tcgattcgcg atgaagccat caaagcattt gtggtgctga atgaaggtga cacattgagc
    32281 gaagaggaat ttttccgctt ctgcgaacaa aatatggcga aatttaaagt gccctcttat
    32341 ctggagatca gaaaagatct gccacgtaat tgctcgggga aaataattag aaagaatctg
    32401 aaataaatga aacagcaggg aactaccctg ccaactaata atcacgctat aaaacaatat
    32461 gcattttttg cagggatgtt gtcgtccctg aaaaagcaaa aatggagaaa aggaatgagt
    32521 gaatcattac atctgacccg caatggatca attctggaaa ttacccttga tcgtccaaaa
    32581 gcgaatgcta ttgatgcaaa aaccagcttt gaaatgggcg aagtatttct aaatttccgt
    32641 gacgatccgc aattacgtgt cgccattatt accggtgccg gagagaagtt cttttccgcg
    32701 ggctgggatt taaaagcggc agcagaaggc gaagcaccgg atgctgactt tggcccgggt
    32761 ggatttgcgg gattaaccga aattttcaat ctcgacaaac cggttatcgc agctgtgaac
    32821 ggctatgcct ttggcggcgg tttcgaactg gcgctggcgg cagattttat tgtttgtgcc
    32881 gataacgcca gcttcgccct gccggaagcc aagctgggca tcgttcctga cagcggcggc
    32941 gtgctgcgtc taccgaagat cctgccgcct gccatcgtca atgaaatggt gatgaccggc
    33001 agacgaatgg gcgcagaaga ggcgctgcgt tgggggatag tcaaccgcgt ggttagccag
    33061 gcggaactga tggataacgc ccgcgaactg gcgcagcagc tggttaacag cgccccgctg
    33121 gcgattgcgg cgctgaaaga gatcttccgc accaccagcg aaatgccggt agaagaagcg
    33181 tatcgctata ttcgcagcgg cgtgttgaaa cactatccgt cggttctgca ttcggaggat
    33241 gccattgaag ggccgctggc gtttgccgag aagcgcgatc cggtgtggaa aggacgttaa
    33301 caaccgtgag ctattacgcc tttgaggggt taattccggt ggttcacccg acggcgtttg
    33361 tccatcccag tgccgtcttg attggcgatg tgattgtggg agccggtgtc tacatcggcc
    33421 cactcgcctc actgcgtggt gactacgggc ggttgatcgt gcaagcggga gccaatattc
    33481 aggatggctg cattatgcat ggctactgcg acactgacac catcgttggg gaaaacggcc
    33541 atatcgggca cggagcgatc ctgcatggtt gtgtgattgg tcgcgatgca ttggtcggga
    33601 tgaacagcgt gattatggat ggcgcggtca ttggcgaaga gagcattgtt gccgccatga
    33661 gctttgtcaa agcaggcttt cacggcgaga aacgccagtt gttgatgggt acgccagccc
    33721 gcgctgtacg cagtgttagt gacgacgagt tacactggaa acggttgaat accaaagagt
    33781 atcaggatct tgtcgggcgc tgtcatgcat cgttacatga aacacagcca ctgaggcaaa
    33841 tggaggaaaa tcgcccccgt ttgcagggga cgacggatgt gacgcctaaa cggtgatctt
    33901 gccggatgcg gcgtaaacgc cttatccaac tctgctggag agcaaccgta ggccggataa
    33961 gatgcgccag catcgcatcc ggcgatagct gtcggatgcg gcataaacgc cttatccaac
    34021 ccggctggag agcaaccgta ggctggataa gatgcgccag cattgcatcc agcaaccagg
    34081 ctgcgtggta tcaacgaaat gacattaacg acgcatactc tttgacaaca tcatctgcca
    34141 cttctgctcg cggttaagtt ccgtgagcgg aacggcagga atacgcggcg tctttcttac
    34201 gccgactaat ttatcccggc tgttattgcg caggcgcgcg taaatttgct catcgatatc
    34261 gaccactttt accagtcgct ggcactggca tccccgtcct tccagcttat tagggatcat
    34321 cttaactgcg cagcttattt ctgcgacttc cgacagaata taagtcaggg tattaaccgc
    34381 cttgctgtgt tcaatatcga aatgaataga gatctctctt gctatcaccc accgattttc
    34441 agccatcatc cattcggcga ttaacaagta gagtggtttt tcaacatatc cttcacacat
    34501 acgaactcca gtttcacaaa ttaaaagtct ggctatgact cgttttgaag caattaaaca
    34561 aggccatatt aaaattgtgg atatatcgat agtatgcaac ttcaccgtcg ataag
//
LOCUS       DAZPLI010000027        34602 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZPLI010000027 DAZPLI010000000
VERSION     DAZPLI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34602
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.027"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..459)
                     /locus_tag="ACHUU3_001268"
     CDS             complement(<1..459)
                     /locus_tag="ACHUU3_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR2081390.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
     gene            complement(537..1286)
                     /gene="btuD"
                     /locus_tag="ACHUU3_001269"
     CDS             complement(537..1286)
                     /gene="btuD"
                     /locus_tag="ACHUU3_001269"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR2081391.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            complement(1286..1837)
                     /gene="btuE"
                     /locus_tag="ACHUU3_001270"
     CDS             complement(1286..1837)
                     /gene="btuE"
                     /locus_tag="ACHUU3_001270"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR2081392.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(1900..2880)
                     /gene="btuC"
                     /locus_tag="ACHUU3_001271"
     CDS             complement(1900..2880)
                     /gene="btuC"
                     /locus_tag="ACHUU3_001271"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR2081393.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            complement(3073..3510)
                     /locus_tag="ACHUU3_001272"
     CDS             complement(3073..3510)
                     /locus_tag="ACHUU3_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HFR2081394.1"
                     /translation="MSDKLERRIERLEGDLSLTRNDLATLAERTTNLSTKADVGEVKG
                     ELKADIAHLKGDLECDIANLKGELKSDTANLKEQLKSDINSLKGELTEAMDKRFDKIM
                     DEMNRRFDKVDDNTKWRWSGIIVPVCTTIFTAAVAVFVAKFVG"
     gene            complement(3611..4111)
                     /locus_tag="ACHUU3_001273"
     CDS             complement(3611..4111)
                     /locus_tag="ACHUU3_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2081395.1"
                     /translation="MEIKKPTKKELYDYLLSKYIEDKCKEEADEINKKSMSRVKKHKE
                     RLMEITPEIFFRFLSEKGVSSVCPSCGSSRLSVPESMDLCWDKNKKPENFNNLPLEEQ
                     SELIKENIKHYVSYAFLGDVKSIPDMRKTYYTLHCLNCGYLSLYRTSAVLKWLEKDKA
                     QDGDNG"
     gene            complement(4114..5052)
                     /locus_tag="ACHUU3_001274"
     CDS             complement(4114..5052)
                     /locus_tag="ACHUU3_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR2081396.1"
                     /translation="MRPEGRKGKRIRKKFKTKSDAVLYERWVLAQQHNNEWKGNSIDR
                     RPLSVLIDLWWKYHGQLMKSGHNTRLKLLRLSEAMDDPCVHKLNTTMLTELRVSRIEQ
                     GIQPSTINREIGALSAMFTALISSGHFLNDNPVQGLKGMKVNEREMGYLSKSECVQLL
                     DALAENPDERLAVEILLSTGARWGEVAALEQRRVLHCRITFSKTKNSKNRTVPISESL
                     FEKIKKRGGKLVFPTLDYSLVRDVIKTVAPDVPDGQAVHALRHTFASHFMMNGGNILT
                     LQKILGHAKIQTTMIYAHLAPDYLQDAVRFNPLGGM"
     gene            complement(5141..5452)
                     /locus_tag="ACHUU3_001275"
     CDS             complement(5141..5452)
                     /locus_tag="ACHUU3_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR2081397.1"
                     /translation="MSIEISKKLKAIRESEGLSQAKFADSIGIAVGTVKQYETGIRGV
                     GTEVLLKITMHPEFKKYTTWLMSNETNEAAGQISPSLSPDGPENTSSSQKSRKTGTQP
                     G"
     gene            5548..5826
                     /locus_tag="ACHUU3_001276"
     CDS             5548..5826
                     /locus_tag="ACHUU3_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR2081398.1"
                     /translation="MAKVLNTYEQADFERLAAFYPYRNEHGLPVLEESLEDYAKRTNQ
                     SILAVRRQADRSVIPVTQEGRNTKRKVNLFAIFLKTIRNAEKYVQMTK"
     gene            5841..6179
                     /locus_tag="ACHUU3_001277"
     CDS             5841..6179
                     /locus_tag="ACHUU3_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001698482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFR2081399.1"
                     /translation="MLKQRRNFCTGTERHANRFTTSASRSNIRYSLSDTHATPDGYPV
                     KQIGEHAWLIEKAGIVIHKCPRNPFTGNRIFALSCGDNQFGQDFTLYEALRTVDRLLR
                     GQSFIKQADL"
     gene            6190..6477
                     /locus_tag="ACHUU3_001278"
     CDS             6190..6477
                     /locus_tag="ACHUU3_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2081400.1"
                     /translation="MTKDNAQGVFIRFIDFRGELLLRASAIDGVTPAGKNGADEATYV
                     YLNGTRLLVELPYQTVREIISEDEKARQVNGDEPYIEIICMDSEAEIQKAD"
     gene            6489..6731
                     /locus_tag="ACHUU3_001279"
     CDS             6489..6731
                     /locus_tag="ACHUU3_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFR2081401.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            6728..6841
                     /locus_tag="ACHUU3_001280"
     CDS             6728..6841
                     /locus_tag="ACHUU3_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2081402.1"
                     /translation="MSIFISWLVLIISVVCAIGIMRIINSVKKIERFFTEE"
     gene            6928..7131
                     /locus_tag="ACHUU3_001281"
     CDS             6928..7131
                     /locus_tag="ACHUU3_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2081403.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVVQVNLIRQVQESRR"
     gene            7128..7373
                     /locus_tag="ACHUU3_001282"
     CDS             7128..7373
                     /locus_tag="ACHUU3_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR2081404.1"
                     /translation="MTILDYIAANPGCSSGEIAAALNTPTTTINAELRRLWRSGLVTR
                     KERKTGGRFSYQVNLMPFGCSNPLTQMFNQLLREIRA"
     gene            7399..7518
                     /locus_tag="ACHUU3_001283"
                     /pseudo
     CDS             7399..7518
                     /locus_tag="ACHUU3_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310657.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7515..7880
                     /locus_tag="ACHUU3_001284"
     CDS             7515..7880
                     /locus_tag="ACHUU3_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2081405.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTV
                     LEIQRLATVCRDTLNGMTK"
     gene            7887..10709
                     /locus_tag="ACHUU3_001285"
     CDS             7887..10709
                     /locus_tag="ACHUU3_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFR2081406.1"
                     /translation="MTAEYIRDWQQPRHAVGREGTGIPAPESALSSWLDAHRAENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRRKGKKSWQAERYARGTLRKIFERLDRTDGHWLTPGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKDRRHEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFATVRKKLNRDGLRWYGIRTAEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVDEWMRSLKRHERRALMRSLRDKQA
                     KNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLTQLGASD
                     PEAAARLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERARRKADLLRGG
                     LTSGNKKQTETGLTNPVNEQKTRSDI"
     gene            10786..11745
                     /locus_tag="ACHUU3_001286"
     CDS             10786..11745
                     /locus_tag="ACHUU3_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR2081407.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGMQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAGFSGYGRVM
                     VVGGGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
     gene            11750..12061
                     /locus_tag="ACHUU3_001287"
     CDS             11750..12061
                     /locus_tag="ACHUU3_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR2081408.1"
                     /translation="MTTPTRRISFYLKPAAVKNESEACAWLGSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWADDEALSVTQTRTERPVPQPVPTAQITSQIAGNIRALFP
                     E"
     gene            12122..12646
                     /locus_tag="ACHUU3_001288"
     CDS             12122..12646
                     /locus_tag="ACHUU3_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2081409.1"
                     /translation="MKKYEYMLMKEALRAGLAPCPVSVEAVVGNTIFRTLRNRVSVKM
                     VHQPPKSTSMIYLPLPEAIKLMRSLKFAIWMQTQINKAVQNTFVKRLIPELAARCAKC
                     TRPALQFDLSLIQQATEAELYRLSPIRDAHTEASEMFRLMFQFNFAVRRTRVQVNRMM
                     QEQEELTQYKGGSQ"
     gene            12643..12882
                     /locus_tag="ACHUU3_001289"
     CDS             12643..12882
                     /locus_tag="ACHUU3_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2081410.1"
                     /translation="MSNSARLQLGFSPLSKTIVLAKMRDLGDGTKRRVGNDRGRDVTN
                     EAAQLVWHLVMAEGGEINWELDDGSRMILRAEKSK"
     gene            complement(13574..14620)
                     /locus_tag="ACHUU3_001290"
     CDS             complement(13574..14620)
                     /locus_tag="ACHUU3_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR2081411.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDTQPTS"
     gene            complement(14620..16371)
                     /locus_tag="ACHUU3_001291"
     CDS             complement(14620..16371)
                     /locus_tag="ACHUU3_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR2081412.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDATATGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAEMHFLGTSAATAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTCFS
                     TPSTETHEAYAYWNGDRWNEKKAAHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFRNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTYRYNVEHIGIDATGGHGDAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            16526..17362
                     /locus_tag="ACHUU3_001292"
     CDS             16526..17362
                     /locus_tag="ACHUU3_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFR2081413.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVESVATS
                     QADIIDRFNALETRHQQDSQKITLLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            17386..18438
                     /locus_tag="ACHUU3_001293"
     CDS             17386..18438
                     /locus_tag="ACHUU3_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFR2081414.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPAVQQR
                     FENASKDSTELTKKINVIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLK
                     NRKYQCEQVNYDTFISYPQLDAWSAHSDFQSRISAQIARQVALDRIMIGFNGTSHADE
                     SNFSTNKLLQDVNVGWLEHIRTNASERVMNDVTLTSRNMDNTVAHAGKYANPDALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            18484..19284
                     /gene="gpM"
                     /locus_tag="ACHUU3_001294"
     CDS             18484..19284
                     /gene="gpM"
                     /locus_tag="ACHUU3_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFR2081415.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKADIKKALLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYAVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSVFIEPLDTLWEI
                     VANQDMPDEVRAKLCKACAFARRGLSDADSMASSLKLLREAMHLNQNAGVKREIATLS
                     RALKKADSAAAPEDASAQQAQDESSESKKTTRKPATRKTTATQKAKRG"
     gene            19386..19880
                     /locus_tag="ACHUU3_001295"
     CDS             19386..19880
                     /locus_tag="ACHUU3_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFR2081416.1"
                     /translation="MSMVARTEPGPAEDDITDTEDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            19880..20077
                     /locus_tag="ACHUU3_001296"
     CDS             19880..20077
                     /locus_tag="ACHUU3_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFR2081417.1"
                     /translation="MKVKTLEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCRQVFLEA
                     GQEVEMPEPEKKKQREMIQLW"
     gene            20071..20373
                     /locus_tag="ACHUU3_001297"
     CDS             20071..20373
                     /locus_tag="ACHUU3_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2081418.1"
                     /translation="MVTGFFQRVSDWITFWASVTATTIGVMTISEKIALAGLLLGSLS
                     ALHAWRHRRLMETAMQRRNELIASILAQSDARPLHDSERQALNILRDNDTHDKAAR"
     gene            20354..20902
                     /locus_tag="ACHUU3_001298"
     CDS             20354..20902
                     /locus_tag="ACHUU3_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR2081419.1"
                     /translation="MIKLPVKRYAVAAIVALGFSIAPHKLRTSPEAQIRIATWEDCRA
                     TPYRDIAGVMTVGCGSTGHVENRLYTETEVAGRWVNDMQHAENCINQNFSGNAMPQSA
                     FEAMTDAAFNLGCRNLMWFRDKNRNYHRTTIWKHAQAHNWPDMCNRLTDFVNSGGERS
                     QGLVNRRTDFRAWCLRDTGGQS"
     gene            20899..21372
                     /locus_tag="ACHUU3_001299"
     CDS             20899..21372
                     /locus_tag="ACHUU3_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2081420.1"
                     /translation="MKVRYILMMALTVLTLLTITLWQTFRLGQEDGHNRYTQAQLDDY
                     SRTLKEAGTIISTASGVLRDVMTARQNRQQEGEIRREQLRNDITTDACARAVPDARFT
                     ERLHQHAERVRAAAVVPFHPGRADRPRTSPAVSGDAIMGKHRDMERQPHGRTGNL"
     gene            21444..21911
                     /locus_tag="ACHUU3_001300"
     CDS             21444..21911
                     /locus_tag="ACHUU3_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2081421.1"
                     /translation="MLKTDSLREAMTRSCRWCHANPEKFTIFVESGNIETTGETPSFV
                     YRYQIVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLPEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            21904..22539
                     /locus_tag="ACHUU3_001301"
     CDS             21904..22539
                     /locus_tag="ACHUU3_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFR2081422.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTRRVSMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            22536..23117
                     /locus_tag="ACHUU3_001302"
     CDS             22536..23117
                     /locus_tag="ACHUU3_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR2081423.1"
                     /translation="MSAELMRLLSNIIRTGIISEVDEESWCVRVRSGELETGWLRWNT
                     TRAGDFNVWFPPSPGEQVAIACIGGNPETAMIIGSLWSDAIPAPGKSLKEIVVSAPDG
                     AVFRYDADAGALSASGMKTATLQASISVTLNTPVVECTDLLRTATLDVTKGGTMSGNI
                     THSGGDFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            23114..23464
                     /locus_tag="ACHUU3_001303"
     CDS             23114..23464
                     /locus_tag="ACHUU3_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFR2081424.1"
                     /translation="MTTRYTGMNPDGTGNLNDMAHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSATVIALTRWEPRIALNAIDVVWKAGGHAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            23468..24364
                     /locus_tag="ACHUU3_001304"
     CDS             23468..24364
                     /locus_tag="ACHUU3_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR2081425.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIAAYPAEQREVVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEAARACMLSHSAGTDLDNLAGNMNTKRLV
                     ITPATDTTDVVMENDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            24357..24887
                     /locus_tag="ACHUU3_001305"
     CDS             24357..24887
                     /locus_tag="ACHUU3_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFR2081426.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPADLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFNGEPGTFKIDVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIQGDIFA
                     AAATYDGEVITIYPDD"
     gene            24890..26860
                     /locus_tag="ACHUU3_001306"
     CDS             24890..26860
                     /locus_tag="ACHUU3_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2081427.1"
                     /translation="MSTTTRKFKTIITDTGAKKLAQATAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVTLDATHQNHIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPTVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVNYVDDKL
                     KEHEQSRRHPDASLTAKGLVQLSNATNSTSETLAATPKAVKAVMDETNKKAPLNSPAL
                     TGTPTTPTARQGTNNTQIASTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATT
                     MTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAKSTVAAV
                     IEYLGLQETVNQASGALQKNQNGADIPGKDTFTKNIGACRAYSSWLNIGGDSQVWTTA
                     QFISWLESQGAFNHPYWMCKGSWAYANNKVITDTGCGNICLAGAVVEVIGTRGAMTIR
                     VTTPSTSSGGGITNAQFTYINHGDAYAPGWRRDYNTKNQQPAFALGQTGSTVGNDKAV
                     GWNWNSGVYNANIGGASTLILHFNMNTGSCPAVQFRVNYRNGGIFYRSARDGYGFEAD
                     WSEFYTTTRKPSAGDVGAYTQAECNSRFITGIRLGGLLSVQTWNGPGWSDRSGYVVTG
                     SVNSNRDELIDTTQARPIQYCINGTWYNAGSI"
     gene            26863..27396
                     /locus_tag="ACHUU3_001307"
     CDS             26863..27396
                     /locus_tag="ACHUU3_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR2081428.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGVVI
                     KRTYTGEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(27425..27952)
                     /locus_tag="ACHUU3_001308"
     CDS             complement(27425..27952)
                     /locus_tag="ACHUU3_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR2081429.1"
                     /translation="MMHLKNIKAGNAKTVEQYELTKKHGVIWLYSEDGKNWYEEVKNF
                     QPDTIKIVYDANNIIVAITKDASTLNPEGYSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(27954..28940)
                     /locus_tag="ACHUU3_001309"
     CDS             complement(27954..28940)
                     /locus_tag="ACHUU3_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2081430.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNNSDADTDSY
                     MWFETGNNGNEYFKWRHRIIGTRPKDLMNLKWNALSVLVEALFSSEVKISTVNALRIF
                     NSSFGAIFRRSEECLHIIPTRENEGENGNIGPLRPFTLNLRTGRISMGHGLDVTGDIF
                     ANRFAINSSTGMWIHMRDQNVILGRNAVSTDGAQALLRQDHADRKFMIGGLGNKQFGI
                     YMINNSRTANGTDGQAYMDNNGNWLCGSQVIPGNYGNFDSRYVKDVRLGSQQYYGVNN
                     WQTWNFQCPSGYVLTGINVQDTGKNSADNIAGVHYRPVQKYINGTWYNVASI"
     gene            29045..29632
                     /locus_tag="ACHUU3_001310"
     CDS             29045..29632
                     /locus_tag="ACHUU3_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR2081431.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTKTDRPG
                     LKRALKRLQKGDTLVVWKLDRLGRSMKHLISLVGELRERGINFRSLTDSIDTSSPMGR
                     FFFHVMGALAEMERELIVERTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHD
                     RKQLAIIYGIGISTIYRYHPAGESIGTIEKSQETK"
     gene            complement(29668..30156)
                     /locus_tag="ACHUU3_001311"
     CDS             complement(29668..30156)
                     /locus_tag="ACHUU3_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2081432.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDERIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(30169..32976)
                     /locus_tag="ACHUU3_001312"
     CDS             complement(30169..32976)
                     /locus_tag="ACHUU3_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR2081433.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTGQYNNQLERERRALARVT
                     QARERYSRAQETAGKLKTGGALATGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     KNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLAKMGIRGSDAGTALRKSLANLSSPPKAAAEALKELGVSVADA
                     KGKMRPMENVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMGLVDAAGDGFLAKLR
                     KELEGARGEAERTAKVMANNLDGNLKSLGSAWEGLRIRISDLVDGPLRSVTQWLTRVV
                     SKVTALAQAHPALTRQLLIAGGALLATTATVGSLSLVIGVLHGKLATLRLGFSLLTES
                     MNVVRVLPALWGMVTGSVSLLGGAIGALFSPVSLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDVVRDGIIQVFNWFKSLLSPMESSKETLDKCASA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKFGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVD
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVASGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            complement(32963..33118)
                     /locus_tag="ACHUU3_001313"
     CDS             complement(32963..33118)
                     /locus_tag="ACHUU3_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFR2081434.1"
                     /translation="MCFEHIEDFVADIAAIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            complement(33127..33492)
                     /locus_tag="ACHUU3_001314"
     CDS             complement(33127..33492)
                     /locus_tag="ACHUU3_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2081435.1"
                     /translation="MKETKNIDTENTVVADTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGEKNEQATEA"
     gene            complement(33547..34059)
                     /locus_tag="ACHUU3_001315"
     CDS             complement(33547..34059)
                     /locus_tag="ACHUU3_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFR2081436.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGRDIVP
                     DRIRSALGLG"
     gene            complement(34059..>34602)
                     /locus_tag="ACHUU3_001316"
     CDS             complement(34059..>34602)
                     /locus_tag="ACHUU3_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HFR2081437.1"
                     /translation="AYIDHEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNN
                     KEITTIIRRNGFRFWGNRTPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANV
                     KDVIGAIRAKLDSLVTAGKLIGASCWYDVVDNGTTNLRQGRVRIRYKYTPVPPLEDME
                     LYQSFTDEFFGPAFAVLGGA"
BASE COUNT         8689 a         9441 c         8808 g         7664 t
ORIGIN      
        1 tcgtctcgcc tgccagaaat ttttctgcca atagacatta tcaagtgagg aacgcatcac
       61 tcccttgctg gtagaggcgt ggataaattg gttgttggta tcgtaaatac ctacatgcaa
      121 accattttgt ccggaacccg ttttgaaaaa gaccaggtca ccaggcagca actcgtcttt
      181 atcaatttgc gtgccgatag aggcttgttc tttggtttct cggggcagct gcaaatcgaa
      241 acgatcgcgc atcgttacaa ccacaaatcc cgaacagtcc acaccgcgcc gcgtcatgcc
      301 accataacga tacggcgtgc catgccagct ttgtagctgg tcgttcaaac cggcaataac
      361 ggtaatcgaa tcagaaagtc tggcatttgg cggcggtgct ttatggtggc tacacccggc
      421 cagaaacagt gctgtgatca aaataaggca gaaacgcatt ccgtacggtt cctctgtttt
      481 ttattcttgc attaatttag cgtcgtaatt acccgatttt caagatacta atgaaatcag
      541 atggtcgaaa tcagcattct gtgaccttcg atatccagac ggcgaaaatt catcccatag
      601 gcctgcgcca gatttggcgg cgtgagcacc tcttctctgc gtccactggc cagcattttt
      661 ccacctttta gcaaccacgc ccgatgcgca tgacgcaatg tgtggttgag atcgtgactg
      721 ctcatcacaa tcgccagtcc ttgctgacac agagcgctca gaattttgtc taacgcactt
      781 tgttgcgcaa catcaagact gttcatcggc tcatcaagaa gcagcaattg gcctgcggga
      841 ttggcttgtg gtgtgatttg cagcaccacc gcagcaagac gtacgcgttg ccattcaccg
      901 ccggaaagtt gattggtgct acgtccgagt ttgtcatcaa gagccagcgc ccctgcgaca
      961 tcattcagta gttcggtacg cgttttatcg tgctgatgca gtgtcagata gtgccagacc
     1021 ggcattgcaa acggcggcgt ctgctgttgt gaaagatagg cgcgatgcag cgcgagtttt
     1081 gttgcggacc atgcttccag tggttgcccc gcgaactgaa tacttccctt accgctggtc
     1141 attccggcca ttcgtgccag taaggtactc ttacccgcgc cattcggccc caccaggtgc
     1201 aggatctccc cagcccgaac ctcgccagaa agtggcccca ggcgggtaga ttccgcaaca
     1261 tcctgtaact gcatcacaat agacattatt ttgccaacgc cagtttaatg ctttccatca
     1321 caatggggtc ttccggcgtc atatccgggg aaaaacgctg gatgaccaat ccgtccctgc
     1381 caactaaaaa tttctcaaaa ttccataaaa tatcatcagg gtacagcggt gcacgacctt
     1441 tgctgaccat acgggcatag aatccgctct cttccggcgc gactgcggtc ggcgctgcgg
     1501 caatcaattt ttgatacagc ggatggcgtc cttcgccgtt aacttcaatc ttactgaaca
     1561 tcgggaacgt caccccccat gtggtggtac agtaagtttt aatctcttca tcgctgcccg
     1621 gttcttgttc cagaaactgg ttgcacggga atcccagcac cacaaaacct cgatcggccc
     1681 aggctttctg aatattctcc aactgctcat attgcggcgt taagccacac tttgaggcga
     1741 cattgacaat caacagcaca ttaccggcgt acttctccag cgtggtcact tcaccgtcga
     1801 tatctttcac tacggtcgtc agaatggaat cttgcatcgt ttctcctggg tgtggtcagt
     1861 aaaaatctta gcttttaatc atagaccgtc tttttgcggc taacgtcctg cttttaacaa
     1921 taaccagata aacaccggcg cacccaacgt tgcggtgacc acgccaatag gcagctctgc
     1981 ggcagctaat gccaggcgcg ctacaatatc ggccagcagc actgcgctcg cccctgccag
     2041 cgcgcagccg ggaagtaata cgcgatgatc ggttaaacca cacaaccgaa gaatatgtgg
     2101 gatcaccaga ccaataaagc cgatagcacc cgccagcgcc acactgacgc caaccatcca
     2161 gccggtcgct gccaccagca cattgcgcca gaaccacagg ggtaaaccca gttgccgcgc
     2221 cgagatctcg ccaagtgcta acatattcat cggcctggac tgacaacaga tccacaacaa
     2281 cacggggatc aatgccagca tcagccagct ttgccgccag tctacgccgc caaaaccgcc
     2341 catcatccag tacatcagct gacgtaaatc aacagaggtg gaaaagtaga tagcccacgt
     2401 cattagtgcg ctacagataa tccctaatgc aacgccagcc agcaataacc gactggtcga
     2461 aagatgacga cgggcgaaac gtaagagtat taaagtgatg ataagcgcgc cagcaatcgc
     2521 acacagccct agcgcccagt tggggagttg cccttgccca agcaataccg cggcgataag
     2581 ccccacgcct gcgccgttag agacgccaag tagtccaggt tctgccagag gattttcaaa
     2641 caacgcctgc attacagcgc cggatatagc cagcgccgca ccaaccagca atacagccag
     2701 cgtacgtggc aggcgaattt gccagacgaa cagttcgcca cgaggagtaa accagtcacc
     2761 tggcgaaatc cattgttcac cggcgcaaag gcttaagaga agcgccagca gcatcaaaac
     2821 tgacaggcat aataaccagc gaatattttg tcgctgttgt tggcgggcaa gtgtcagcat
     2881 ggtatccgtt ctgctgaagt gtcatggcgt tgattttacg gtgactcttc gacagtgaaa
     2941 agaaaaaagg ccgcagagcg gccataaaca caaacaaaaa tcaataagtt aggtaattat
     3001 caaagactta cagacacaca aaaacacagc caaccacaac aaataacaga gatgtggtca
     3061 atttgtggat catcagccaa caaatttagc aacaaataca gcaaccgccg ctgtgaaaat
     3121 ggttgtgcac actggcacaa taatgccact ccaacgccac ttcgtattat catcaacctt
     3181 gtcaaaccgc cgattcatct catccataat cttgtcaaag cgtttatcca tcgcttcggt
     3241 aagctcaccc ttcaggctgt taatgtctga tttgagctgc tctttcaggt tagctgtatc
     3301 agatttaagc tcacctttca gattcgcaat atcgcattca agatcacctt tcagatgtgc
     3361 tatgtctgct ttgagttcac ctttcacctc accaacatcg gctttagttg agaggtttgt
     3421 agtgcgttca gcaagcgtcg caaggtcgtt tcttgttagc gataaatcac cttcgagccg
     3481 ctcaattcgc ctttcaagtt tatctgacat tccgcctcca ctactgccgc catcgccacc
     3541 atgataaagc acatcacgct cagaatgcga cacactacct gctgaattac cgaaaagatt
     3601 tcttgctaca ttacccatta tcaccatcct gcgctttgtc tttctccaac cacttcagca
     3661 ccgcagacgt acgataaagg ctcaggtaac cacaattcag gcaatgcagc gtgtaataag
     3721 ttttgcgcat atcaggtatg ctcttaacat cacccaaaaa agcataagat acataatgct
     3781 ttatgttctc tttaattaac tcactctgtt cctccagggg tagattgtta aaattttctg
     3841 gtttcttatt tttatcccag caaagatcca tactttctgg aacagataat cgcgacgatc
     3901 cacatgaagg gcagacactg gagaccccct tctcagacaa gaaacggaag aagatttctg
     3961 gcgtaatctc catcagccgc tctttatgtt ttttgacacg actcatcgac ttcttattga
     4021 tttcatcagc ttcttctttg catttatctt ctatatattt cgatagtaaa tagtcatata
     4081 actctttttt tgtaggtttt tttatttcca tagttacatt cctcccaagg gattaaacct
     4141 caccgcatcc tgcaagtaat ccggcgcaag atgggcataa atcatcgttg tctgaatctt
     4201 tgcgtgcccc agaattttct ggagcgtcag aatattgccg ccgttcatca tgaaatgact
     4261 ggcgaaggtg tggcgcagcg catgaacagc ctggccgtca ggaacatcag gtgcgaccgt
     4321 tttgatgaca tcgcgaacca atgaataatc cagcgtcgga aacaccagtt tcccgccccg
     4381 ttttttgatc ttttcaaaca ggctttcaga aataggaacg gtacggtttt tgctgttctt
     4441 cgtttttgaa aaagtgattc gacaatgaag aacacggcgc tgctccagtg ccgctacctc
     4501 gccccatcgc gccccggtcg acagaaggat ttcgacggcc agccgttcat cgggattttc
     4561 agccagtgca tccagcaact gaacacattc agacttactc agatatccca tttcacgctc
     4621 gttaaccttc attcctttaa ggccttgaac ggggttatcg ttaagaaaat ggccggatga
     4681 gatgagtgcg gtaaacatcg cgcttaacgc cccaatctct cgatttatgg tgctgggctg
     4741 tatcccctgc tctatcctgg acacacgtag ctcggtgagc atcgttgtat taagtttatg
     4801 cacgcacggg tcatccattg cctcactcaa gcgcagcaat ttaaggcgcg tgttatgccc
     4861 tgacttcatt agctggccgt ggtatttcca ccacaagtca ataagcactg acagcggacg
     4921 gcgatcaatg gagtttcctt tccactcatt gttatgctgt tgcgccagca cccaccgctc
     4981 atataaaact gcatccgatt tcgttttaaa ttttttgcga atgcgtttgc ctttacgccc
     5041 ctcaggacgc atgtcaagaa gataccctcc cggaattgat tttatgctca ttcgtgaagc
     5101 cccagcgtta caagaccacc atgcccccag cgttccatga ttagccgggc tgtgtgccag
     5161 tcttgcggga tttttgagaa gacgatgtgt tttctggccc atcaggggag agagaaggac
     5221 tgatctgccc agcagcctca tttgtttcgt tactcatcaa ccacgtagtg tattttttaa
     5281 attccgggtg cattgtgatt ttcagtaaaa cctccgttcc cacacctcga ataccagtct
     5341 cgtattgctt aaccgtacca accgcaatac ctattgaatc cgcgaacttt gcctgactaa
     5401 gcccctcaga ttctcgaatt gcttttagct tctttgatat ctctattgac atggtgtgca
     5461 cctgaatact atattggtta tcagttgagt accacttgac gctacaaaga gccacaaata
     5521 gcaccagatt gaataggtta tcacaccatg gcaaaagtcc ttaacacata cgaacaagcg
     5581 gattttgagc gtttggcggc gttctaccca taccgtaatg aacatgggct accggtactt
     5641 gaagaaagcc tagaggatta tgcgaaacgc acaaatcaaa gcattctcgc tgtaagaagg
     5701 caggctgata gatcggtaat tcccgttact caagaaggaa gaaacacaaa acgcaaagta
     5761 aatctcttcg caattttcct gaaaaccatc agaaacgcag agaaatacgt gcagatgaca
     5821 aaataacgag gtgtcatttt atgctgaagc aacgccgtaa tttttgtacc ggaacagaac
     5881 gccacgctaa ccgtttcact accagtgcat cacgcagcaa catccgctac agcctgagtg
     5941 atacacacgc aacgccggat ggctacccag taaaacaaat cggcgagcac gcctggctga
     6001 ttgagaaagc tggaatcgtg atccacaaat gcccacgcaa tccgtttacc ggaaaccgca
     6061 tttttgcact gagctgtggc gacaatcagt tcgggcagga tttcacatta tacgaagcac
     6121 ttcgcacggt tgatcgtctg cttcgcgggc aaagttttat taaacaggct gatttataac
     6181 aggtgcttta tgaccaaaga caatgcacaa ggtgtattta tccgttttat tgattttcgc
     6241 ggtgaactgt tattacgtgc atccgctatt gacggagtga ctccggcggg taaaaacgga
     6301 gccgacgaag ccacttacgt ttatctgaac ggcacgcgac tgcttgtgga acttccgtac
     6361 cagaccgtac gagaaatcat cagcgaagat gaaaaggcac gccaggttaa tggcgatgaa
     6421 ccctatatcg aaattatttg tatggattca gaagctgaaa tacagaaagc agattaaagg
     6481 gcgttgtgat gggcaaagaa tataaaactc tcattaacaa agcacttgag cgtttttatt
     6541 ttcgcttaag tgcatcaggc gctcatgctg aacgtgcggc ccgtgactca ttgaccagag
     6601 caatccgaag tctgtatgac gtggcttttt acgctgatga tctggatgca cttaacgaac
     6661 tttccgagct gatctgtgcc gcagaatgcg gggaacatat tgaaccgtat aagctgggaa
     6721 atatcgcatg agtatattta tttcatggct tgttctgatt atttcggtgg tctgcgccat
     6781 tgggattatg cgaattatta attcagtaaa aaagattgaa cgctttttca ctgaagaata
     6841 accgcgcaaa taagacccca ggttaaataa gaaaatgtga aaacaatccg cattcgcgga
     6901 ggtattcgca cacgccaagg aggcgtaatg gcaattaagc attttcctgt cgttcgtttc
     6961 acctccagag gacgtgaata cgaagttgac gaacgcctga ttaccacaat cgacaaacac
     7021 cgttcagaaa aggatgcaca tcatatctat ctcactgacg gcacttactt ctgcgccacc
     7081 aacgtggtgc aggtgaatct tatcagacag gtacaggagt cacgcagatg accattctgg
     7141 actacatcgc tgccaatccg ggttgtagca gtggagaaat cgccgcagca ctgaatacac
     7201 caaccacaac cattaatgcg gagctacgcc gtctctggcg cagcggttta gtcacaagaa
     7261 aagagcgcaa aacaggcggt cgcttttctt atcaggtaaa cctgatgccg tttgggtgta
     7321 gcaacccact aacccagatg ttcaaccagc tactgaggga aatcagagca tgagcacctc
     7381 caactgccgg aaaccacgtc gggcttcagc agctcatccg gcagcaaaac aaactccatt
     7441 aattcctgtt ccgggccttt cctgcacctt gcggcgggag gccttcgcac atctgtaaca
     7501 agaggattgc cgcaatgatt ctcgccaacg actttcttga atacctgctc aacacagagc
     7561 gtgatcttgc cgctcgcgtg cgtgatcgtt atgacatgta cctgaaatcc ctgcctgtac
     7621 cgcagctcgc tgacggaaag attgttattg atggtcgcta catgattgac agccacgagg
     7681 gaaattacag gctttaccgc attgaaggtg gcaccccgtc cgttattggc atttaccagc
     7741 gcccatcctc tgcaatcgtc gatgtgattg ccgacagcat ccgcatcaca catcgccatg
     7801 ccgacacaga agacaccgtg ctggaaattc agcggctggc tacagtctgc cgcgacaccc
     7861 tgaatggcat gacgaagtaa atcactatga cggcagagta catcagggac tggcaacaac
     7921 cgcgccacgc agtggggcgt gaaggaacgg ggatccccgc tcctgaatcc gcgctttcct
     7981 cctggctgga tgcccaccgg gcagagaacg agcgccgcca ggaaatggct gatgcggcgt
     8041 tctccgccac gccactgggc aacctgatta ataaaagcct ggacgcacag gaaaaacagg
     8101 acaaaaccat cacactggca ggagacgcca gaaaacaggc acgcggcgcg gtggatgaag
     8161 ccatggcctc gctgcgcctg ctgccgtcct atctgcgcga tccgcttatt cgccacctct
     8221 ccttcctgcg caaaaaacag gaagccgatc gccggaaagg caaaaagagc tggcaggcgg
     8281 aacgctatgc acgcggaacc ctgcgcaaaa tattcgaacg tctggatcgc actgacggac
     8341 actggctgac accgggttat cgctcccttg ccggacgtga acgcctggac gatttgcttt
     8401 acctgccgca actcaacaaa caccagatac agacgctggc caccatgacg gcggcgatgt
     8461 tcagcagcac cttcgaaaaa ctctgcgatg gttttggcgc gactgatggc gagctgacca
     8521 tggatgtaac gctgaaggcg tatcagatgc tggcccgcat ggcgttacac ttacacgcca
     8581 tgcctccgca ttatgacgca ctgacaacag acaaagaccg gaggcacgaa ccggacacag
     8641 aactgctgcc gggcgcaatc cttcgcctga cctgtgcgga atggtggaaa cgcaaactgt
     8701 ggctgttacg ttgcgagtgg agagaagaac aacttcgcgc cgcctgtctg gtttccagaa
     8761 aaacatcgcc ctatctgagc caggacgcgt taagcgagtt tcgcgcacag cgcgagaaaa
     8821 cacgcgattt cctgaaaagt ttcatgctgg aaaatgaaga cgggttcacg attgatctcg
     8881 agacggtgta ttacgcggga gtaagtaacc cggttcaccg taaggcagaa atgatggcca
     8941 ccatgaaggg gctggaactt ctggccgaag cccgtggcga caaagcggtg tttctgactg
     9001 tcacctgccc gtcaaaatac cacgccacaa cggagaacgg tcatccgaat cccaaatgga
     9061 acggggccac aatgcgcgac tccagcgatt acctggttaa cacgtttttt gcgacggtcc
     9121 gcaaaaaact gaaccgcgac ggcctgcgct ggtatggcat ccgcacggcg gagcctcacc
     9181 atgacggcac cgtgcactgg catatgatgg tctttgctca tccggaagaa atcgacacca
     9241 ttgtgtccca cacccgcgat attgccattc aggaagatcg tcacgagctg ggcgatgaca
     9301 taactccgcg ctttaaggcg gagtatgtag acggctcaaa aggcacgcca accagctaca
     9361 tcgccaccta catcgggaaa aacctggaca gccgcgccgt ggatggcatc gacccgaaaa
     9421 cgggcaagcc acgcgttgac cacgaaaccg gaaaatcaat ggccgagagc gtggaacgcg
     9481 ccatcggctg ggcgcgcctt caccgggtcc gccagttcca gttctttggc atcccctccc
     9541 gccaggtatg gcgtgaactg cgccgccttg ccagccagat ggcacgcaac ccggaaggcc
     9601 cgcaacggct gaaggatgac gcaatggatg cggttcttgc cgccgctgat gccggatgtt
     9661 ttgccaccta catagagaaa cagggcggcg tacttgttcc acgcaaagac tacctgattc
     9721 gcaccgccta cgacctcgcc gatgagctga acgattacgg cgaacagagc gtacagattt
     9781 acgggatctg gtcaccactc atcggggaat cctcccgtgt gtgcacgcat ccggataact
     9841 ggaagctggt aagacgcaaa ccggaagcgg aagacagcgc ccgcgaaaat ggttttgacc
     9901 ttcagggcgg ccctgccgcc ccttggactc gtggcaataa ctgtccccgt gtacaggaaa
     9961 cggacaacaa cgggacagaa cagccggaag aacggccagc accgtggccg cagctccctg
    10021 acggcgttga tgtggatgaa tggatgcgct cactgaaacg gcacgaacgc cgggcgctga
    10081 tgcgttcgct tcgtgacaaa caggcaaaaa acagcagtga tgaaatgcag agctggacac
    10141 agagccgcaa acagcagcgg cctttgcctg ataaccacga attactcgct aaagaatggc
    10201 gggagtctgc tgaatctctc ggcctgcata tcggtgaaca acagatgcag cacctgttac
    10261 ggggcggcag tctgtacgtt gacggcagca tcattgcacc gcagggattt gaaattgtac
    10321 gcaaaccgga tacccgcccg gacagccgaa tcacgcagct ctggcagcgc ctgagccgta
    10381 atcatggcgt aagcagcacg gagatccgcc ataacccggt cgccagctat ctgacacagc
    10441 tgggggcatc agaccctgaa gccgccgcac gcctggcatc cacacttcag caggaccaga
    10501 acaccatgaa aacacccgtt accgtgcttt ctgacatgct gcgcgccatc cgcgacgcag
    10561 agcacgcaca gagaatcagt gaaaccactg aacgcgcccg ccgcaaagca gacctgctgc
    10621 ggggtggcct gaccagtgga aacaaaaaac agacagaaac gggactcacg aatcccgtaa
    10681 atgagcaaaa aacgcgcagc gatatatgaa gcgcgcacaa aacaggcaaa aacgggattt
    10741 cagaatcccg taaacgatta attaatcaac ataaggaaaa gcgacatgaa aatttgtatc
    10801 gacgacggct ccaccaacat caagctggca tggactgaga acggcgaacg ccgcaacgcc
    10861 atcagcccga acagcttcaa gtcggaatgg tctgcgccgt tcggtggcat gcagcccgcg
    10921 aactacatgc ttgatggcgt gcgctatggt tttgatccgg tcagcgatcg ctttgtccag
    10981 acaaccgaca cgcaatacca gtacagcgat gtaaatgtca ttgccattca tcacgcgctg
    11041 gtcaaatcag gcatcacgcc acaggaggtg gatgtggttg tcaccctgcc actgagcgag
    11101 tatttcgaca caaacgcaca gccggacatg gccaacatca accgcaaaaa agcgaacgtt
    11161 atgcgcccgg tggagtacca gaacggcgaa gcattcacta tccgtaacgt gcgggttatg
    11221 cctgaatcca ttccggctgg ctttaaggca ctggctgaca tgagtccgtt tgaatccctg
    11281 ctgattgtgg atttgggcgg aaccacgctg gatgtggcaa aggttcaggg gcaactggca
    11341 ggtatcagcc aggtgttttg cgatccacac gtaggcgttt ctctgatggc cgatgccgta
    11401 ctgtcggtga tggccactaa cggtatgcgc accagtcacc acatcgccaa taccattatc
    11461 gaacatcgcc atgatgaagc ctggctgcgc cagcacatcc acaatgacgc gcattacgcc
    11521 agcctgatgg cggttattcg tgaaaaggaa gaaacactga aacaacgcgt gatccgcgcg
    11581 ctggcgggtt tttcgggtta cgggcgggtg atggttgtcg gtggaggggc ggagattgtg
    11641 gcacccgcta tccgcgaagc ctgcggagtt aatgcgactt tcatcgcgga cggggtgcca
    11701 cagtttgctc tggttaatgg gctgtacgca atggacaagg agtaaaccaa tgacgacacc
    11761 aaccagacgg ataagtttct atctgaagcc tgcggccgtc aagaacgaaa gcgaagcatg
    11821 cgcctggctg ggcagcctta caccagaagc ccgcaaaagc ggccaacgcg tggcttttct
    11881 ggccgggctg gcacttctga aaatgaatcc agcagaggct taccgactgg ctgcatgggc
    11941 tgacgatgag gcgttatcgg tgacacaaac caggacagaa cgccccgtgc cacagccagt
    12001 accaaccgca cagataacca gtcagatagc agggaatatc cgggcattat ttcctgaatg
    12061 acgaatttga cggcttttaa ttttatcggg gtgcactgtc gccccgcacg aggaaaacac
    12121 gatgaaaaaa tacgaataca tgctaatgaa agaagcactg cgggctggtc tggctccctg
    12181 tcctgtatcc gtagaagctg ttgttgggaa cacgattttc cgaacgctgc gaaatcgcgt
    12241 atccgtgaag atggttcacc aaccacccaa atctaccagc atgatctatc tgccgctgcc
    12301 cgaggctatc aaacttatgc gttcgctgaa atttgcgatt tggatgcaga cacaaatcaa
    12361 caaagcggtg caaaacacct tcgttaagag actaataccg gagcttgctg ccagatgtgc
    12421 aaaatgtacc agacccgcac ttcaatttga tctgtcattg attcaacaag ctacggaggc
    12481 agaactgtac cgcctgtcac ctatccgtga tgctcacacg gaagcatcag aaatgttccg
    12541 gctcatgttt caatttaatt ttgccgttcg acgcacacgc gttcaggtca atcgcatgat
    12601 gcaggagcag gaagagttaa cacagtacaa agggggcagc cagtgagtaa ttcagcacga
    12661 ctacagcttg gtttttcacc gctatcaaaa accatcgtat tggcaaaaat gcgtgactta
    12721 ggagacggaa caaaacgccg cgtaggaaat gatcgcggtc gcgatgtaac caacgaggcc
    12781 gctcaactcg tttggcatct agtcatggca gaaggcggtg aaattaattg ggagctggat
    12841 gatggttctc gtatgatttt gagggccgag aagagtaaat agctcaaggg ggattaatgc
    12901 taaatgatga tgaagaagag ctgcttatgc aggaatggtc gctcggtgat tatgataatg
    12961 gtgaaaatgg gtgcccgcat tgtggccgtt atcgtttatg tatctgccag aatggaaaac
    13021 acagatgtga aaagtgcaat tggtctccgg aacttaatga ctacgtgccg atcgagtggt
    13081 agaggtaagt cacactaggt gttttttagt tgattttata aatgcacaat agtgcacaaa
    13141 tttgcacaat ttttttgaac gactttttgc ccttccggcc cgcgtggcgg ctggatccgt
    13201 caaggatccg tgcatgcaca aaaaaacgcg ttttttctgc gcgcaggtga cgggggaaca
    13261 gcccgcgttt cagggggtaa atagcattcc ctgaacgatg tcgcagagat acaacagaat
    13321 ggctgtattt ctcacgctga gcgtgaaaaa gacgtgaggg cttttgattt gatggggtga
    13381 aaggtaaggc cgtcaaaatc gcactgagac ggcgagaata tgcagtcaac gaggtgggat
    13441 tgcgtaagag tctgactgtc gatgatggca atcagcagga aagcgtcgtg aaattatctg
    13501 actgatacag gagctggaga gtcggggcat aaatttttta tgccccggcg aagcagcaga
    13561 caagcgaagc gcgtcaggat gtgggctggg tgtctaacag tgcgtaaggg ttaaagcgga
    13621 tcacctcttc gccaagccag tcattgatgt gcttcatggc ctccatgacg ggcatcagct
    13681 cgttaattgc gtaaacccgc gcggccttct ccacatcacc aaacgcactt ttttcgcccg
    13741 gcatcgcccc catcagttgc ggcggaacgc ggtgcgcagc cagcacatca tcacgggatg
    13801 ccgccttaac attcatgaac tcatcctttg cggtgatctg ctggaacggc aaaatttgca
    13861 ccccctcttt gcccccgttg ggcgcatgaa tgagcacgtt tttaaacgca ccaccaccac
    13921 gtgccccctg tagcgtttct ttcagggagt ccatgctttc gcggtttacc tgcgctgcac
    13981 cgatgtagat gatgcacccg gcgtgggatc cgttgtcgta gtacagtttt ctgaacatgt
    14041 ccgccgaatg agaaaggctg gccgagagta atgcgccaag atattccggc atgccgtaga
    14101 tttcctggtt aatatccgga ttcatcaggt ggcacacttt gccaggacga aactgaaacg
    14161 catccttgcc atcctgcaca taccaccatg attcaagatc gcttccgcgt cgcatgtatt
    14221 tcgccagggc gtgccgtaat ttaagcggtt cgccgagcat attgctccga agctcaagga
    14281 atgcgttacc gaacacaaac cagtccagcg ccagcgccga gaaatcctgc cgggaaagca
    14341 gcgggtgcgg aatatagcag ccgagcaata cattgcgctt aaagtaaagc gcagactgat
    14401 gccaggacgt ttgccgggca gctcttgcca gaccgtacca gtccaccggg gtttcatacc
    14461 accgcccgtt atcagcacag tacatattgt ccagcaggtc atgcccggtc aggcggtaag
    14521 gaccatcaaa tgtgaatgca ctgagcgacg attctttcct gagcgcatca gcgagatcaa
    14581 tgcgtgaact catgcgcact tttttatttt ttctgctcat cagaactcca taaccgtgaa
    14641 acgctcgttt tctccttcgc cgccaatcgg ttcgttaatg acagcaagca tggttgccca
    14701 cgcaaggtcg ccgtggctga tccccctcgc gcggtccgtt tcgtaagtga taaaaccgcc
    14761 cggtgttttc accttacgca cggcgttaaa ggccgcaacc agctcgcgtt cggcgcgatc
    14821 gtattcccac cgcccggcac gcattatttg cagcattttc agtaccagcg accgttttga
    14881 tgacagcgtg aaggtgtaag ggatggcggc gggaaaaaac cgtttcacta tctgatagac
    14941 ggcatccccg tgcccgcccg tcgcatcaat gccgatgtgt tccacgttgt agcgatacgt
    15001 gaactcttca ataactctgg cctgctcttc aaattccagc ccctgaacgc gtcgcgtctc
    15061 caccgttctg aaacgaccac caggaacagc cggaggaacc accacggaca cagcgccgct
    15121 gtcgccgtta ccactgctgc cgtttgcgtc atacccaatc cataccggac gattccccat
    15181 cgggcggggg gcaaagggtt tccagtcttt ccagtcgtcg tatccgtcaa caccgcagcc
    15241 aatcaggata ttcaggttaa atgccgattc cccttcgcgg acaaactcac acatatagag
    15301 attgcggaac tcgtcttcgg tgttttcatc acgaatttca tcaatatcgg tgtgtttcca
    15361 gccgtgatta accacatctt ccagcgtgac aatttgccgc cacgtccggt cagggcagat
    15421 aagcccgtta tgcagcgttt tccagtccac agaaaaacgc tggcgtttat gcgcggcctt
    15481 tttctcgttc cagcggtcgc cgttccagta ggcgtatgcc tcgtgcgttt cggtggatgg
    15541 cgtggagaag caggtgcgcc gcagtccgct gagggttgcc atagcgccag ccaccttgcg
    15601 cagttcagca aagcgactga cccagaaaaa ttcatcaaaa taaaaattgc ccgtgtaaga
    15661 ttgcgccgtc gcagcagaag tgccgagaaa atgcatctct gcgccgttgg agaggatgat
    15721 tttatcgccc cctttcagct ccacatcgac ttcagccgcg gccttctgaa taatgctttt
    15781 aaactggaac gcctgacgac gcgacgcaga caaaaaaatc tggttacgct ggtaaggttg
    15841 tgccacatcg tcacgcagcg ccatcagcag tgcttcctgt gcaaaatacc aggtcgcccc
    15901 aatctgtcgg gatttcagga tcatcctgtt acgtatcccg gcttccctgc aaagcgtcag
    15961 ggagtcaaac cagccccgct gatgccactc cagcctgctg atgatttttt cccgcagtgc
    16021 ggcaatctgt tccggcgtga aatgattttt gagttttttc gcccggcctt tctttcctgt
    16081 ggccgtcgca tccggctggc catcatgcag ttttttaagc tgccgggtca gcaggtctat
    16141 ttccttaaag tcaccacctg ttttattctg tttttcagta agctggatga ggcgcgcatc
    16201 gatggactgc gtgacacgct gcacgggtgg cgtttcatcc cactggtcgc gttttttcca
    16261 cgcataaatc gtgttcgggt ttattcccat cagacgtgat atttctgcgg gcggataacc
    16321 ctgccagtaa agttgccgcg cacgctggcg cacaaaagcg tcctgaatca ttgctccccc
    16381 tgagtaatta caggaagatt acccgcgcgc gaaaccgttc tccttaaccc cctgttctgg
    16441 ccgttttctt acaacaaaag ccctttgtat cagcctgtta cgctttgcca tcatgactga
    16501 agaaccagtc agaggggcaa aaactatggc taatgaaaaa aagacatccc gcaaaaagtt
    16561 tcgcgtggct gtctccggat caactgttga tggccgtgaa atcagtccgg tgcatctgcg
    16621 tgaagccgcc gagaacttca acccggatgt ttacgctgcc cgcgtgaacg ttgagcacta
    16681 tctctcgcca tgcccgtcaa gcgaattttc cgcaatgggc gatgtcaccg cactgagtac
    16741 ggaagacatt acggaaggtc cgctggccgg acgtactgcg ctgtatgcag aaatcgaacc
    16801 gaccgagcgc atgaagcagc ttgtcgcgga cggcaagaaa atctattcca gtatcgaact
    16861 gcacccgcag ttctccgtta acgggcgcgc ctatctggtc gggctggcga tgaccgacac
    16921 cccggcaagc ctgggcactg agcgcctgaa attcacggca cagcaacgtc aggcggtgat
    16981 gacgttcaac agtatccagg gtgaagcacc gcttatctcc gaagccatcg agtctgaaat
    17041 catcgaaatg gcagaacaac gccaggaaga aggcacccag tggtttaacc gcgtaatggg
    17101 gattattggt cgtggccgca aagcggatga cgccagtttc tcccgtattc aggaagcggt
    17161 ggaaagcgtc gcaacgtcac aggccgacat tatcgaccgt tttaatgcgc tggaaacacg
    17221 ccatcagcag gacagccaga aaatcacttt actgaccacg gagctgacag cactgaagga
    17281 aaaactgcgc acgcaggacg gcgatccgca gaaccggttc accgcaacgg gcgcagcctc
    17341 cgaccagctg gctgacttct gataagacaa aggagcaaat tttttatgaa tctggtgatg
    17401 tcagatatta cccgcaacaa gctgggttgc tatatggcgc agcaggcgtc gcttaacaat
    17461 atcccggttt ctgcactggt atcgcgattt accgtggaac ctgcggtgca gcaacgtttt
    17521 gaaaacgcct caaaggatag caccgaactt acaaaaaaaa ttaacgtgat cggcgtgacc
    17581 gaccagaaag gcgaaaaaat cctcctggac accacaggac cgattgcacg cacaaatacc
    17641 agttatgacg gaacaaaacg ccgtaacccg aataacgtgg ttgatctgaa aaaccgcaaa
    17701 taccagtgcg aacaggtgaa ctacgacacg tttatttcgt atccgcagct tgatgcctgg
    17761 tcggcacaca gcgattttca gtcccgcatc agcgcacaga ttgcccgaca ggtggcactt
    17821 gaccgcatca tgatcggttt caacggcaca tctcacgcgg atgagtccaa cttcagcacc
    17881 aacaagctgc ttcaggacgt taacgtgggg tggctggagc acatcagaac caatgccagc
    17941 gagcgcgtaa tgaatgacgt gacgctgact tcccgcaaca tggacaacac cgtggcgcac
    18001 gcgggtaaat acgcgaaccc tgatgcactg gtacaggacg cgcgctcatc tctgctggat
    18061 gaatggcaca aggaagctga cgacctcgtg gtgattatgg ggcgcaacct gtttaactcg
    18121 ctgcgtctgc cagtgctgaa cagcatcagc ggccagaatc ccaatgcgga attacttgcc
    18181 gggcagctca tcctgtcatc gcgcaccatt ggcgggctgg gtgtgttcct tgcaccgttc
    18241 ttcccggatg caacgatgct gatcacctca ttcaacaacc tgtcgattta ctggcagaaa
    18301 ggttcaatgc gtcgtctgat gaaagacgaa ccggaataca accgcatcgc cacctaccag
    18361 tccatcaatg acgcttatgt cgttgaagac tatggcaagt gcgcgatggt cactggcctg
    18421 aagttcgccg acagctaatc aactcacggc gggcatcatg cccgcctgta acggagagaa
    18481 aaaatgatta ctcctgcaca gcaacactgg cagaacgtga tggcacagcg cgcaggccgg
    18541 gcgaatgaag gtgtggacca cgccgcgcgt accgcgcatg aagaggtgct gtatcgtctg
    18601 cgtctggcac aggcccggct taagggcgta caggccagaa gcgcgaaagc cgacatcaaa
    18661 aaagcgttgt tgccggactt ttccggctgg attgagggaa cgctggaggc tgacggcggg
    18721 caacaggacg aagtgattgc cacgctgatg gtgtgggcga ttgactgcgg cgatcttccg
    18781 cttgcgctgc gtattggtgc gtatgcggtc cgtcacaacc tcatcatgcc ggataacttt
    18841 ggccgtactg ctgccacagt gctgaccgaa gaaatctgca atccggtact gacgcaggcc
    18901 gggacggatg ccgacgcgga tttgtccgtc tttatcgaac cactggacac actttgggaa
    18961 attgtcgcca accaggatat gccggacgaa gtgcgcgcca aattatgcaa ggcgtgcgcc
    19021 tttgcccgcc gtggcctgag cgatgcggac agcatggcct catcactgaa gctgctgcgc
    19081 gaagcgatgc atctgaacca gaacgcaggt gtgaaacgcg agattgcaac cctttcccgc
    19141 gccctgaaaa aagccgattc cgcagccgca ccagaagacg ccagcgcaca gcaggcgcag
    19201 gacgaaagca gcgaaagtaa aaagacaacg cggaagcctg caacacgaaa aaccaccgcg
    19261 acgcagaagg cgaagcgcgg ttaacgactg accccgtcag cgggcggcgt gcgcggtgtt
    19321 ccggtttggc tccgtgaccg tttacaccgc gcacccaccg cccgattttt tcaggagtga
    19381 accccatgag tatggttgcc agaactgaac ccggacccgc agaggacgac atcaccgaca
    19441 ccgaagatgg cgatacccgt atttcagcgg gtgcattctg gccggatatt gtgctgcgcg
    19501 agctgcgtct ggcggtacga ctgccgggcc gcgtgaccac ctcccgcctg ctgcataccg
    19561 ccaccggggc cgtggcacac gttacccgcg agctggaagc atggcagcag gaacagcagg
    19621 cggctggcca tcagacgctg gccgatgttc cggcacctgt aattaacgga gaaagcgtca
    19681 atctctggca ctggcgcaat gctgtttata ccgccacacg cgccctgatt ctggagcgtt
    19741 accgcgatgc ggacacaacg gacaagggcg accgccgggc ggacgcactg gatatacaga
    19801 catcggattt gtggcgcgat gtgagctggg ccatctctga cattctgtgc cgcccgcgaa
    19861 tctttgcgga gttgtgctga tgaaagtgaa gacactggaa ggcgacaccg tggattcgct
    19921 ctgtttccgg tactacggca cgacgcaggg cgtcaccgaa aaggtgctgg atgccaaccc
    19981 cggactctgc cgacaggtat ttctggaagc cggacaggag gtggaaatgc cggagccgga
    20041 gaagaaaaaa caacgagaga tgatacagct atggtaacag gtttttttca gcgggtgagt
    20101 gactggatca ccttctgggc atcagtcaca gccaccacaa taggcgtgat gaccatcagt
    20161 gaaaaaatcg cccttgccgg gttgttactc ggttctctgt ctgcattaca tgcctggcga
    20221 catcgccgcc tgatggaaac cgccatgcag cgtcgcaatg aactcatcgc cagtatcctg
    20281 gcgcagtcag acgcacggcc acttcacgac agtgagcgtc aggctctgaa cattctgcgg
    20341 gataacgata cacatgataa agctgcccgt taaacgttac gccgtagccg ccattgtggc
    20401 gctggggttc tccattgccc ctcacaaact ccggaccagc ccggaagcgc aaatccgcat
    20461 tgccacatgg gaggactgtc gcgcaacgcc ttaccgggat attgccgggg taatgaccgt
    20521 ggggtgcggc tcgaccgggc atgtggaaaa tcgtctgtac accgaaacgg aagtcgccgg
    20581 gcgctgggtt aatgatatgc aacatgcaga aaactgcata aatcagaact tcagcggcaa
    20641 cgccatgcca cagtccgcgt ttgaggcaat gacagatgcc gcctttaatc ttggatgcag
    20701 aaatcttatg tggttcaggg ataagaaccg taactaccac cgcaccacta tctggaaaca
    20761 tgcacaggcg cacaactggc ctgacatgtg caacagactg actgacttcg tgaacagcgg
    20821 cggcgaacgc tcacagggac tggttaaccg tcgtactgat tttcgtgcat ggtgcttacg
    20881 tgacaccgga ggccagtcat gaaagtgcgt tacatcctga tgatggcact gaccgtcctc
    20941 acgctgctga ccataacact ctggcagaca ttcagactgg ggcaggaaga cggacacaac
    21001 cgttatacac aggcgcaact ggacgattac agccggaccc tgaaagaggc cggcaccatc
    21061 atcagcaccg catccggtgt actgcgtgac gtcatgacag cgcgccagaa ccgacaacag
    21121 gaaggggaaa tccgacgtga acagctacgc aacgatatca cgacagatgc atgtgcccgc
    21181 gctgttcctg acgctcgctt tactgagcgg ttgcaccagc acgcagaacg cgtccgcgcc
    21241 gccgccgtcg ttccgtttca tcccggaagg gctgaccgcc cccgtacttc ccccgccgtt
    21301 tccggtgacg ccatcatggg gaaacatcgg gatatggagc gacagcctca tggacgcact
    21361 ggaaacctgt aacgccgata agcgggccat tgaattactg gaacagcgca ggctgcaacg
    21421 actgaacaac gaggacaaca accatgctga aaactgattc cctgcgtgaa gccatgaccc
    21481 gttcatgccg gtggtgtcat gccaacccgg aaaaattcac cattttcgtg gagagcggca
    21541 acattgaaac gaccggagaa accccatcgt ttgtttaccg ctatcagata gtgatgtttg
    21601 tcatggatta cgccggagag ctggacgacc tcacgctgcc gctgctggcg tggttatccg
    21661 aaaatcagcc acagttgttg ctcaatccgg agcgtaatca ggacatcaaa ttttccgccg
    21721 ttatcaatga cgatgacagc gccgatctcc tgtttacgct ccccctgcgg gaacgcgttc
    21781 gcatcacgcg cagcagtcag ggcacaccgc aggcagaaca cctgccggag ccaaaacccc
    21841 gcctgccctc ttccgaaggc gactggtcgc atgtattcca ggatgtgacg tggggggaaa
    21901 gcgatggata aggcattcac ccgcgtggat gaaacctttg aggctatccg cgacagcctg
    21961 aatcagcagg ccattaataa catcgccaga aagctggcac aggatttacg ccgcgcccag
    22021 caggcgcgta tccggtcaca gaaagcgccg gacgggaccg catggacacc acgcagacgc
    22081 cgcgtaaccc ggatacagga gcgcattcgc tttatctgga ataacgaagc acgcacgctg
    22141 aaaaactggc atcacgacac ggggaaatac gggcgaacca tcaccgggtg ggatgaggat
    22201 aaaaacaata tccgcacgtt ttaccgggat gacatcgacc gttttctgga aatacgcacc
    22261 cggcgcatca accaggacag caccaggcgc gtctccatgt tcgtaaaact gcgcaccgcc
    22321 cgctacctga aagcccgtgc agatgcttcc ggtgtgacgg tgggttacag cggcgtggcc
    22381 gcacgtattg cacgcgttca tcagttcggt gagcgcgatc aggttgcgcc gggcattttc
    22441 accgattacc cggtacgtga gctgctgggt atcagccagg cagatgagcg cctgatttat
    22501 aacacggtgc tgggccggat tgcggaggct gtacgatgag cgcagaactc atgcgactgc
    22561 tgagcaatat catccgcacc gggatcatct ctgaagttga tgaggaatcc tggtgcgtgc
    22621 gcgttcgcag cggcgaactg gaaacaggct ggctgcgctg gaacaccacg cgcgcgggag
    22681 acttcaacgt gtggtttccg ccatcaccag gcgaacaggt ggcaattgcc tgcatcggcg
    22741 gcaacccgga aaccgccatg ataatcggca gcctgtggag tgatgccatt ccggcacccg
    22801 gcaaaagcct gaaagaaatc gtggtcagcg cgccggacgg cgcggtgttc cgctacgacg
    22861 cggacgcagg cgcactgagc gcaagcggca tgaaaacggc cactttgcag gcatccatca
    22921 gcgtgacact gaacacgcct gtcgtggaat gcacagacct tctgagaaca gcgacgcttg
    22981 acgtcacaaa agggggaacg atgagcggca atatcacgca cagcggcggc gatttcacct
    23041 caaacggcat cacagtgcat acgcataagc acggtggcgt taaaggtggc agcgattcga
    23101 caggaggccc gcagtgacaa cccgctacac aggaatgaac ccggacggga cgggaaacct
    23161 gaacgatatg gcgcacctga aacagtcagt cagggacatc ctgaccaccc cgctggcaag
    23221 ccgggttatg cgacgggaat atggcagcct tgtgcctgat ttgattgacg aacccatgaa
    23281 taacaccacg cgtctgcaat gcatgagtgc taccgtgatt gcactgacac gatgggaacc
    23341 acgcattgcc ctgaacgcca tcgacgttgt ctggaaggca ggaggccacg ccggggtgac
    23401 gctgtcgggc actgtcatgc agaccatgca gaatgttgaa ttaaccatca cgctgaggga
    23461 gtaaatcatg cctgccgttg acctttccca gttaccggaa cccgccatca tcgcggagcc
    23521 tgactttgag gcaattctgg ctgacacaaa ggccatgatg attgcagctt atcccgccga
    23581 acagcgtgaa gtcgtctccg ccgcactgga actggaatcg gaacccctga acgttatcgc
    23641 tcaaaccatg tcgtttcgtg aaatgctgtt acgccagcgg gtcaatgagg ccgcacgcgc
    23701 ctgcatgttg agccacagcg ccggaacaga cctggacaac ctcgcgggca atatgaacac
    23761 aaagcgcctg gttatcactc cggcaacgga taccaccgac gtggtgatgg agaacgacac
    23821 ctcgctgaga ctgcgggcgc agcgggcgta tgacggcctg agtgttgcag gcccgtcagg
    23881 cgcatacgag tattttgccc gcagcgccag cggtctggtg cgcgatgcgc gggccatcag
    23941 tccgtctccg gcaaatgtga cggtttccat cctgtccact gagggcgacg gcacagcaac
    24001 ggaggcgttg cttaataccg ttcgcgccgt tctgaatgca gaggataccc gcccggtggc
    24061 cgaccgcctg accgtacaga gtgccagaat cgtgacatgg cggctgaatg caaaactgta
    24121 cttttacccc ggtccggaat ccgaacctat tctggctgcg gctgaatcgt cgttcaggaa
    24181 gtggctggct gagcaggggc ttatcggtca ggacgtggcg ttgtccgcca ttgctgccgc
    24241 actgcatgtg cacggtgtgc aacgcgtgga gataatcgaa cccacacaga atatggccat
    24301 cagcgacata caggcggcgc gctgtgagtc attcaccatc agcgaaggtg ggcgtaatga
    24361 gtaattcact gttaccacca tcagccagca atttcatgcg ttgtgccgaa gccgtcggaa
    24421 cacgcattac agacattccg gcagacctca acacgctgtg gtcgccggac acctgcccgg
    24481 tgcatctgct gccttatctc gcctgggcgt tttccgttga ccgctgggat cgcaactggc
    24541 cggaagagac aaagcgacag gtgattcgtg atgcatggct gatacaccga cacaaaggaa
    24601 ccatcagcgc cctgcgacgg gccattgagc cgctgggata cctcattcgc gtgtctgagt
    24661 ggtgggagtt caacggcgaa cccggaacat tcaagattga tgttggcacg ctggacagtg
    24721 gcgtgacgga ggaaatgtat ctggaaatgg agcggttgat tgctgatgcc cgcccggtca
    24781 gccgccacat gacagggctg aatatcattc aggagattca gggagatatt ttcgcggcag
    24841 cagcaactta cgacggtgaa gtcattacca tttatccgga cgattaagca tgagtaccac
    24901 aacacgaaaa tttaaaacca ttatcaccga tacgggtgca aaaaaattag cgcaggcaac
    24961 cgcgccagat gggaaacctg tccgcctgac acatatggcc gtgggtgacg gcggcggtac
    25021 tttacccacg ccagacagta agcagacccg tcttgtgcat gaggtgtggc gacataccgt
    25081 taatcgcgtc accctggacg caacacatca gaaccacatt attgcggagc tggttattcc
    25141 tcctgaaacg ggcggattct ggatccggga aattggtgtg tttgatgagc acggcgattt
    25201 gatcgcggta ggcaatactg ccgaaagtta caagccaacc gttgccgaag gatccggacg
    25261 tgcacaaaca tttcgcacca ttctgaccgt atccagcact gccactgtgg cgcttaccgt
    25321 ggataacacc atggtgatgg ccacagtgaa ttacgtggat gacaaactga aagagcatga
    25381 acagtcacga cgtcacccgg acgcctcgct gaccgcaaaa ggccttgtcc agctcagcaa
    25441 cgccaccaac agcacgtctg aaacgctggc agcgacacca aaagccgtta aggcagtaat
    25501 ggatgaaacg aacaagaaag ccccattaaa cagcccggca ctgaccggaa cgccaacaac
    25561 accaactgcg cgacagggaa cgaataatac ccaaatcgca agcacggctt tcgttatggc
    25621 tgcgattgcc gcacttgtag actcttcgcc tgatgcactg aacacactga acgagctggc
    25681 ggcggcgctg ggcaacgacc cgaattttgc gaccaccatg actaacgcgc ttgcgggtaa
    25741 gcaaccgaaa gatgccactc tgacggcgct ggcggggctt gctactgcgg cagacaggtt
    25801 tccgtatttt acggggaatg atgtcgccag cctggcaacc ctgacaaaag tcgggcggga
    25861 tattcttgcg aaatcgaccg ttgccgccgt tatcgaatac ctcggtttac aggaaacggt
    25921 aaatcaggct tctggcgcat tacagaaaaa ccagaacggc gcagatattc cggggaaaga
    25981 taccttcacc aaaaatattg gtgcctgccg cgcatatagc tcatggctga atattggtgg
    26041 cgatagtcag gtctggacaa ccgcgcaatt tatttcgtgg ctggagagtc agggagcatt
    26101 taaccatcct tactggatgt gcaaaggctc atgggcttat gcaaataata aggtcattac
    26161 agatacaggt tgcggaaata tttgtcttgc aggtgctgtg gtggaagtta ttggcactcg
    26221 cggcgcaatg accatacgcg ttaccacgcc gagcacgtcc agcggtggcg gaattactaa
    26281 cgctcaattc acttatatta atcatggtga tgcttacgct cctggctggc gacgagacta
    26341 caacacgaaa aatcaacaac ctgcatttgc tttagggcaa acaggaagca ctgtcggaaa
    26401 tgataaagct gttggctgga actggaatag cggggtctat aacgcaaaca ttggtggcgc
    26461 atcgacatta atcctccact tcaatatgaa tacggggagc tgccctgctg tacagttccg
    26521 cgtgaattac aggaacggcg gtatttttta tcgttcagcg cgtgatggtt atggctttga
    26581 agctgactgg tcagagtttt acaccacaac ccgcaaaccc tctgcgggag atgttggtgc
    26641 atatacgcag gcagaatgta actcaaggtt tattacaggt attcgccttg gtggtctgtt
    26701 atctgttcag acatggaatg gtcccggatg gtctgacagg tcaggttatg ttgttaccgg
    26761 ttccgttaac tcaaacagag atgaactaat tgatacaact caggcaaggc caattcagta
    26821 ttgcattaat gggacgtggt ataacgcggg gagtatttaa cgatgatgca cttaaaaaac
    26881 atttctgctg gtaatcctga aacaaaagag caataccagc taacgaaaca atttaacatc
    26941 aaatggcttt atacagagga tgggaaaaac tggtatgagg aacaaaagaa ctttcagcct
    27001 gatacgttga aaatggtcta tgaccacaac ggcgttatta tttgtattga aaaggatgtt
    27061 tcagcaatta atccggaagg cgcaagcgtc gttgaggttc ctgatattac agcaaatcgc
    27121 agggctgata tttcaggtaa atggatgttc aaagatggcg tagtgataaa gcgaacttat
    27181 accggggaag agcagaggca acaagcggaa aatgaaaagc aaagcctgct acagctcgtc
    27241 agggataaaa cccagctatg ggactcacag ctacggctgg gcattatttc cgacgagaat
    27301 aaacaaaaat taaccgagtg gatgctctat gcgcagaagg tcgaatccac agacacctcc
    27361 agcctgccag taacgtttcc cgaacaacca gaatgaaaca aggcccgata tcgggcctta
    27421 atttttactc aggcttttgt ggccattctg gctttgccgt atccacacgg ctgaccagaa
    27481 cactgtagcg ttcccatgct tccagtcgtg tgcgttcctc gtccgtcgcc atattcagcc
    27541 tgacagcgcg ttccagcggc tggatgactg attcagcttc ggaaagcaat gcggcctttt
    27601 gtgattcggc ctgttgttgc tgttcgtcag ccgtataaat ccgtttaacc acagctccgt
    27661 ccttaaacat ccacttaccg gaatcatcag cacgacgatt agctgtaata tcaggaacct
    27721 caacgacgct ataaccttca gggttaagcg tggaggcatc tttggtgatg gcgacaataa
    27781 tattatttgc atcgtaaaca atctttattg tgtctggctg aaagttcttc acttcctcat
    27841 accagttttt tccgtcctca gagtaaagcc agatgactcc gtgcttcttt gttaactcat
    27901 actgctccac tgttttagca ttacctgctt ttatgttctt taagtgcatc atattaaata
    27961 ctcgccacat tataccaagt gccatttata tacttttgca ctggcctgta atgaacgcct
    28021 gcaatattat cggcagagtt ttttccggta tcctgtacgt taatccccgt cagtacatag
    28081 cctgacgggc actgaaaatt ccatgtttgc cagttgttca ctccataata ttgctgtgaa
    28141 ccaagtcgaa catctttcac atatctggaa tcaaaattgc catagttgcc gggaataact
    28201 tgcgagccgc aaagccagtt cccgttatta tccatgtacg cctgaccatc ggtgccattg
    28261 gctgtccttg agttattaat catgtagatg ccaaattgct tatttcccag accgccaatc
    28321 ataaatttgc ggtcggcatg gtcctgacgg agcaaagcct gagcaccatc agtggatacc
    28381 gcattacgtc ccaaaataac attctggtca cgcatatgaa tccacatgcc ggtactactg
    28441 ttaattgcaa aacggtttgc aaatatatcc cctgtaacat caagaccatg ccccatgctt
    28501 atccggccag ttctgagatt aagcgtaaag gggcgtagtg gccctatatt accattttcc
    28561 ccttcattct ctcgtgtagg gatgatatgc aggcattctt cagaacgacg aaaaatggca
    28621 ccaaaagatg aattaaatat cctcagtgca ttgactgtcg atattttcac ttcactgctg
    28681 aaaagggctt caacaagaac agacaaagca ttccatttaa gattcatcag gtctttaggc
    28741 cgggtgccaa tgatgcggtg tctccatttg aaatattcat tgccgttgtt gcctgtttca
    28801 aaccacatgt aggaatcggt gtctgcatcc gaattatttt taaacccaat ctttgcccag
    28861 tcagtatttc gaatccaggc aaggattgag tcgttttcaa aagtaagtcc accggacaac
    28921 gtatcgccat ttttttgcac ggcgttacca gccttgttta ccgtttcctg taaaccgagg
    28981 ttttagataa tggccgtttc cggcctgcat ggcatgattt gcgcttttgg acgggagatt
    29041 cagcgtgctg attggctatg taagggtatc aacaaatgac cagaatacag acctgcaacg
    29101 aaacgctctt gtttgtgcag gatgtgaaca aatatttgaa gataaattaa gcgggacaaa
    29161 gacagaccga ccgggattaa aacgcgcttt aaagcgcctt caaaaaggtg acacgctggt
    29221 tgtctggaaa ctggatcgcc tcgggcgaag catgaaacat ctgatttctc tcgtcgggga
    29281 actacgggag cgaggaatta attttcgcag tctgaccgac agcatagata catcttctcc
    29341 aatggggcgt tttttcttcc acgtgatggg tgccctggct gaaatggaac gtgaattaat
    29401 tgttgaacgt acactggccg gactggcggc agcacgcgca cgggggcgca caggcggacg
    29461 tcgaccgaag ctgacaaaag aacagcatga gcaaatagca aggctgatca aaaacgggca
    29521 cgacagaaaa caactggcaa taatttacgg cattggtata tcgacgattt atcgttacca
    29581 ccccgcagga gaatcaatcg gaacaataga gaagagtcag gaaacaaaat aaccgctaat
    29641 ctgaccatta gcggtttttg tgttaaatca gaacaaccct ttaactgaac tggccgcgct
    29701 gttaagggat gatgttacct tatctttgaa gccggacagc atatcgctga acgatgagga
    29761 ttgcaggcgc tcccgcaaat cctcatcaca gcgttcaaga gtcagtgaaa attctatctt
    29821 tttcgcctta ccgtagcgat caaactcgga gcgggtcgta ttcgtttcgg tcaggacata
    29881 catgccgtaa atctgcccga cgccatcaat cagaggccag ggtcgtcctg tatacgcctg
    29941 cgtggtcagc agcgacagcg acacttcgcc acctgtaatt tcaggataaa gcacaccaga
    30001 aagaacgatg cgctcatcac ctgcaccgat atactgccag cttgccgaac ggttaacgcg
    30061 ttcatttttc acatgccgcc agcttttgtt ttgctgtaac tgctgatgcg gcaatgtacg
    30121 cagctcaaaa acaaacatgc cgtagatcat catcatggcc atgactcctc aatctttatc
    30181 gtaaaaactg ccacgcccgg cacgggcgcg ccgttccatt tctgccctga ccatttcacc
    30241 gaccagtttc gccagttcgc ggggattctg cgtaacaacg ttatgcagat gaacatgaat
    30301 ttcaccacca aatccggagg caacaggctc ccggttacgg gaagttacag gaactgatgc
    30361 cactggagag cgtatggcct ccgccaccgg gcgggagctg gccgcaacaa cagggaccag
    30421 cgccggaggc agcggagccg ggaccacggg tgtgatatta attgcggggg caggcttact
    30481 gacctgcgca atcttccgct cctgccactc cccacgaaca gcaagtgcgc ggggcaggtt
    30541 cttaaagaca atatcgccgg ggccaatgcg ttttttcgtc tcatcaacca gcttacctgt
    30601 gttatcagca attttgctga gtctgcgtag cgtcccggta ttgctgtctg tgagcggttt
    30661 gttgtctttg ggtttatcac ctccggtgcc attgccattt tccacaggct tcggcggatt
    30721 gattttcgcc aggtccccct gaagcaaggc aaccttgtcc tgaagaatgg ccgcacgctg
    30781 tgcgtcttcg attttcttgc gcgccctttc cgcttcatcc ggaaggacgc caaatttttc
    30841 aagtatccac gccagcgtat ccagtagcat ttttgcaggt gtcagaacaa gttgtaacgc
    30901 accgccaaga acgttaccga atatctcgcc agcactggca catttatcca gcgtttcctt
    30961 gctggactcc atcggtgaca gcagcgattt aaaccagtta aagacctgaa taatcccatc
    31021 gcgcacgaca tcaaaaacag ggccaaaccg ttcaaaggtt tcgcgcaacg gggtcagcct
    31081 ttccataatc ccgctgaaca ccccggcaaa aaatgccctg atgggatccc agtatttcca
    31141 gataagaacg gcagctccgg caagcgcagc cacgataaga ctaaccggac tgaacagcgc
    31201 cccgatagcg ccaccaagta aagaaacgga acccgtcacc attccccata atgcaggcag
    31261 aaccctgaca acattcattg attcggtcag gagagaaaaa ccgagacgca acgtagccag
    31321 cttcccgtga agcaccccaa taaccagaga caacgaacca accgttgctg ttgtcgccag
    31381 cagtgcgcct cccgctatca gtaactggcg cgtcagtgcc gggtgggcct gcgccagcgc
    31441 cgtcaccttt gagacaaccc gcgtgagcca ctgcgtgaca gaacgcagcg gaccgtcaac
    31501 cagatcactg atgcgaatac gcagaccttc ccatgcactg ccgagtgatt tcaggttgcc
    31561 atcaaggtta ttggccataa cctttgccgt gcgttctgcc tcaccgcgcg cgccttcaag
    31621 ttcttttctc agtttggcta agaagccgtc acctgccgca tcaacgaggc ccataaatga
    31681 tgtgaaagcc tcttctccgg caatgtcctt aaagaacgat acccggtcaa cctccccgta
    31741 tttgcgggtg gctttataga gatcggccag cacattctcc atcgggcgca ttttgccttt
    31801 ggcgtcagca actgacaccc ccagctcttt cagtgcttct gccgctgcct ttggcggtga
    31861 tgacagattg gccaggcttt tacgcaacgc tgtacctgca tcactccccc tgatacccat
    31921 cttcgccagc acgcccgcca tcgctgcggc ctgctccagc gatattccca gcttacccgc
    31981 caccggacct gcatatttca tggtttcgcc cagtgcgcga aggtcagtgt tggtacgggt
    32041 aaacgctgcg gtaagtgtgt caccgacccg gtccatctgg tcagcagaga ggccgaactg
    32101 cgtcaggata ttggaaccaa tatccgccgt ctcgccgagg tccataccgc cagccgttgc
    32161 catgctcagc acgccgggaa gcgcagcctg aatggcctgc ggtgtgaaac cagccattgc
    32221 aagaaatgcc tgtccactgg cggcatcgcc tgcggtgaac tgcgtttcag agccaagttt
    32281 taacgcctgc tcacgcagcg ccttaaactg cgggctgttt ttatcgattc gcgtcagtgc
    32341 ctgaacacgg gacatctctt tcccgaaccc gatagcgggc tgcaaaaaac gcccggcagc
    32401 atagccgccc gctgccgccg cacctgttgc cagcgcacca cctgttttca gttttcccgc
    32461 tgtttcctgc gcgcgtgaat accgctcacg cgcctgcgtt acacgcgcaa gcgcccgccg
    32521 ttcgcgttca agctggttgt tgtattgtcc ggtgcgtctg atggcctgct ggatggtgtt
    32581 atcgctgcct gtcagggaaa tgccgtggcg tttcagctct ccgccaagtt cccgcatttt
    32641 ctgaatttcc cgtgtgcgcg attcattcag gcgttcaagc cgggtgctta actgctgcat
    32701 cagcttttgt tgtttttcgc tgagcactgt acccgtgcgt tgtaactgat taagggcgtt
    32761 aagctggcgt cgtgctttca cgatgcccgc atccgcttta ctgacagcgt cacgggcgcg
    32821 ctcaaatgaa cgcgcctgac gctcgagatt tttgatcgcc ccctgcgttc gctggatgga
    32881 gtcaccaaac tgccccatca ggcggcgggc gttttcggca ggccgggtca gcctgtcaac
    32941 ggcgctgaaa gcgacccgga tatcaagagt cttcattgtc tgcattcccg ctgcgaagtg
    33001 ccgcccgctc acgccagcta accacttcgc cgggcgtcat catgaagatt tcggcgggcg
    33061 accagttaaa aatggcggca atatccgcca caaagtcttc tatgtgctca aagcacacaa
    33121 ccgtgatcag gcttccgtcg cctgttcgtt cttctcgcca gagtccgcac cgctcaaaaa
    33181 atttacggca accacacata actgaataaa gtcacgggat gccatttttt tgatcgtcac
    33241 ttcatccagt cgcggtgatg tcacgcgtga cagcagcgta aacatggatt ccgctttcaa
    33301 attcagcaca tcagacagcg acaaatctcg cagagatcca gcctgctcaa tagccccggt
    33361 gatctccaca tacgtgattt tttcgccgcc tcgctcaatt ggttgggtaa gttttacgcc
    33421 acgctcactg gtttctttca cagtgtcagc aacgaccgtg ttttcggtat cgatgttttt
    33481 cgtctctttc atcaggaaac tcctttcagt cagaggcgac gcactgcgcc gcctgcatat
    33541 tacttatcag ccaagcccga gcgcggaacg gatgcgatcg ggcacaatgt ccctgccgtc
    33601 cttccggtaa atgaagttca gcaggtcaat ctcccacaac gggcgatcgt taacactcag
    33661 cttgtagtag gtgtttttaa tggcgtaagt gtgtgatgtg gcttcgccct gtttggcttc
    33721 ccccatatca atttccgtca cacgtccgcg catttcgact tcatacaggt cgctttcagc
    33781 gtcggtgtag tattcacccg caaaacgcag cagcgtaccg tcaatcgtgc cgccatactt
    33841 aaggaacagc tcacgaactg cgccccccat gacaaagctc gcatcaagcg cggagtcatc
    33901 caggccgaga tcaatactta ccgctcccat catgccacca ccccggtagc tgtcggtttt
    33961 gcgcgtcagc ttaggcagag tgacggacgt caccttaccc acttcgtttt caccatccac
    34021 aaacagcgta aaaaagcgaa gatgttttgg tacagccatc aggcacctcc cagcaccgca
    34081 aatgcgggac caaagaattc atcagtaaac gactggtaaa gctccatgtc ttccagcggg
    34141 ggaacaggcg tatatttgta gcgaatacgc acgcgcccct gacgtaaatt cgtggtgccg
    34201 ttatccacca cgtcatacca gcacgacgcc ccaatcagtt tcccggcagt aaccagtgaa
    34261 tccagttttg ccctgatggc accgataaca tccttcacgt tcgcaggcgt cagtggactg
    34321 tcgatggttt caaactgcgc ttccgcaatt gaatcagcca gcacctgtgc ggttcgggta
    34381 tacacctcaa agatgtaggc gttcgtttcc ggtgtgcggt tgccccagaa gcggaacccg
    34441 ttgcgacgaa taatggtcgt gatttctttg ttgttgaggc tgttggcatc actgtcttcg
    34501 gcctgcaacg accagaacac atgcctggac atccccagca catttttaac cggaacgttg
    34561 gacagcgatt tgtgccagcc ctgctcatgg tcaatgtacg ca
//
LOCUS       DAZPLI010000028        34371 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZPLI010000028 DAZPLI010000000
VERSION     DAZPLI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34371
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.028"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..313
                     /gene="srlB"
                     /locus_tag="ACHUU3_001317"
                     /gene_synonym="gutB"
     CDS             <1..313
                     /gene="srlB"
                     /locus_tag="ACHUU3_001317"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR2081438.1"
                     /translation="SDQMLITFREGAPADLEEYCFIHCHGELKGALHPGLQFTLGQHR
                     YPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHVAGPVPDDIAPGSVLKFESVK
                     E"
     gene            317..1085
                     /gene="srlD"
                     /locus_tag="ACHUU3_001318"
                     /pseudo
     CDS             317..1085
                     /gene="srlD"
                     /locus_tag="ACHUU3_001318"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            1191..1550
                     /gene="gutM"
                     /locus_tag="ACHUU3_001319"
     CDS             1191..1550
                     /gene="gutM"
                     /locus_tag="ACHUU3_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR2081439.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            1617..2390
                     /gene="srlR"
                     /locus_tag="ACHUU3_001320"
                     /gene_synonym="gutR"
     CDS             1617..2390
                     /gene="srlR"
                     /locus_tag="ACHUU3_001320"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR2081440.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            2383..3348
                     /gene="gutQ"
                     /locus_tag="ACHUU3_001321"
     CDS             2383..3348
                     /gene="gutQ"
                     /locus_tag="ACHUU3_001321"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR2081441.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(3345..4859)
                     /gene="norR"
                     /locus_tag="ACHUU3_001322"
     CDS             complement(3345..4859)
                     /gene="norR"
                     /locus_tag="ACHUU3_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR2081442.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            5046..6485
                     /gene="norV"
                     /locus_tag="ACHUU3_001323"
     CDS             5046..6485
                     /gene="norV"
                     /locus_tag="ACHUU3_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR2081443.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            6482..7615
                     /gene="norW"
                     /locus_tag="ACHUU3_001324"
     CDS             6482..7615
                     /gene="norW"
                     /locus_tag="ACHUU3_001324"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR2081444.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(7743..9995)
                     /gene="hypF"
                     /locus_tag="ACHUU3_001325"
     CDS             complement(7743..9995)
                     /gene="hypF"
                     /locus_tag="ACHUU3_001325"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR2081445.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(10148..10675)
                     /gene="hydN"
                     /locus_tag="ACHUU3_001326"
     CDS             complement(10148..10675)
                     /gene="hydN"
                     /locus_tag="ACHUU3_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR2081446.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(10823..11833)
                     /gene="ascG"
                     /locus_tag="ACHUU3_001327"
     CDS             complement(10823..11833)
                     /gene="ascG"
                     /locus_tag="ACHUU3_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR2081447.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            12093..13550
                     /gene="ascF"
                     /locus_tag="ACHUU3_001328"
     CDS             12093..13550
                     /gene="ascF"
                     /locus_tag="ACHUU3_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFR2081448.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWICS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            13559..14983
                     /gene="ascB"
                     /locus_tag="ACHUU3_001329"
     CDS             13559..14983
                     /gene="ascB"
                     /locus_tag="ACHUU3_001329"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFR2081449.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(15097..15516)
                     /locus_tag="ACHUU3_001330"
     CDS             complement(15097..15516)
                     /locus_tag="ACHUU3_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR2081450.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(15513..15824)
                     /locus_tag="ACHUU3_001331"
     CDS             complement(15513..15824)
                     /locus_tag="ACHUU3_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFR2081451.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(15986..16759)
                     /locus_tag="ACHUU3_001332"
     CDS             complement(15986..16759)
                     /locus_tag="ACHUU3_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2081452.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            complement(16784..17254)
                     /gene="hycI"
                     /locus_tag="ACHUU3_001333"
     CDS             complement(16784..17254)
                     /gene="hycI"
                     /locus_tag="ACHUU3_001333"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFR2081453.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(17247..17657)
                     /gene="hycH"
                     /locus_tag="ACHUU3_001334"
     CDS             complement(17247..17657)
                     /gene="hycH"
                     /locus_tag="ACHUU3_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFR2081454.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(17654..18421)
                     /gene="hycG"
                     /locus_tag="ACHUU3_001335"
     CDS             complement(17654..18421)
                     /gene="hycG"
                     /locus_tag="ACHUU3_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFR2081455.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(18421..18963)
                     /gene="hycF"
                     /locus_tag="ACHUU3_001336"
     CDS             complement(18421..18963)
                     /gene="hycF"
                     /locus_tag="ACHUU3_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFR2081456.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(18973..20670)
                     /gene="hycE"
                     /locus_tag="ACHUU3_001337"
     CDS             complement(18973..20670)
                     /gene="hycE"
                     /locus_tag="ACHUU3_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR2081457.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(20688..21611)
                     /gene="hycD"
                     /locus_tag="ACHUU3_001338"
     CDS             complement(20688..21611)
                     /gene="hycD"
                     /locus_tag="ACHUU3_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR2081458.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            complement(21614..23440)
                     /gene="hycC"
                     /locus_tag="ACHUU3_001339"
     CDS             complement(21614..23440)
                     /gene="hycC"
                     /locus_tag="ACHUU3_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFR2081459.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(23437..24048)
                     /gene="hycB"
                     /locus_tag="ACHUU3_001340"
     CDS             complement(23437..24048)
                     /gene="hycB"
                     /locus_tag="ACHUU3_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR2081460.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(24173..24634)
                     /gene="hycA"
                     /locus_tag="ACHUU3_001341"
     CDS             complement(24173..24634)
                     /gene="hycA"
                     /locus_tag="ACHUU3_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR2081461.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            24876..25196
                     /gene="hypA"
                     /locus_tag="ACHUU3_001342"
     CDS             24876..25196
                     /gene="hypA"
                     /locus_tag="ACHUU3_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR2081462.1"
                     /translation="MELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSLAFCFDLVCRG
                     SVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVADDGLQIRRIE
                     IDQE"
     gene            25200..26072
                     /gene="hypB"
                     /locus_tag="ACHUU3_001343"
     CDS             25200..26072
                     /gene="hypB"
                     /locus_tag="ACHUU3_001343"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR2081463.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            26063..26335
                     /gene="hypC"
                     /locus_tag="ACHUU3_001344"
     CDS             26063..26335
                     /gene="hypC"
                     /locus_tag="ACHUU3_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR2081464.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            26335..27456
                     /gene="hypD"
                     /locus_tag="ACHUU3_001345"
     CDS             26335..27456
                     /gene="hypD"
                     /locus_tag="ACHUU3_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR2081465.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            27453..28463
                     /gene="hypE"
                     /locus_tag="ACHUU3_001346"
     CDS             27453..28463
                     /gene="hypE"
                     /locus_tag="ACHUU3_001346"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR2081466.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            28537..30614
                     /gene="flhA"
                     /locus_tag="ACHUU3_001347"
                     /pseudo
     CDS             28537..30614
                     /gene="flhA"
                     /locus_tag="ACHUU3_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(30651..31004)
                     /locus_tag="ACHUU3_001348"
     CDS             complement(30651..31004)
                     /locus_tag="ACHUU3_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR2081467.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(31081..31215)
                     /locus_tag="ACHUU3_001349"
     CDS             complement(31081..31215)
                     /locus_tag="ACHUU3_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2081468.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            31291..33852
                     /gene="mutS"
                     /locus_tag="ACHUU3_001350"
     CDS             31291..33852
                     /gene="mutS"
                     /locus_tag="ACHUU3_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR2081469.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            33958..34371
                     /locus_tag="ACHUU3_001351"
                     /pseudo
     CDS             33958..34371
                     /locus_tag="ACHUU3_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
BASE COUNT         8189 a         9411 c         9109 g         7662 t
ORIGIN      
        1 cagcgaccag atgctcatca cctttcgtga aggcgcgcca gcggacctcg aagagtattg
       61 cttcattcat tgccacggcg agttgaaagg tgcactccat cccggtttgc aatttacgct
      121 cgggcagcat cgctatccgg tgaccgctgt tggcagcgtg gcggaagaca accttcgcga
      181 actgggtcat gtcaccctgc gcttcgatgg tttaaacgaa gcggaatttc cgggcactgt
      241 ccatgtggca ggccctgtac ccgacgatat cgcgccggga tcggttttga agtttgaatc
      301 tgttaaggag taaaaaatga atcaggttgc cgttgtcatc ggtggtgggc aaaccttagg
      361 cgcgttcctg tgccacggtc tggctgccga ggggtatcgc gtcgcggttg tcgatattca
      421 gagcgacaaa gccgcaaatg tggcacaaga aattaacgcc gaatatggtg aaggaacagc
      481 gtatagtttt ggtgctgacg ccaccagcga gcaaagcgtc ctggcgctct ctcgtggggt
      541 agatgaaatc tttggtcgcg tggatttgct ggtctacagc gccggaatag caaaagcagc
      601 ctttatcagc gacttccagc tcggcgattt tgaccgtgaa tctggtgggt tatttcctgt
      661 gtgcccgtga attttctcgt ttgatgatcc gcgacgggat tcagggtcgc attattcaga
      721 tcaactcgaa atccggcaaa gtgggcagca aacacaactc tggctacagc gcagcgaaat
      781 ttggtggcgt cgggctgact caatcactgg cgctggatct ggcggagtac ggcattacgg
      841 tgcattcact gatgctcggt aacctgctga aatcgcctat gttccagtca ctgttacccc
      901 aatacgcgac caagctgggt atcaaaccgg atcaagtcga gcagtattac atcgacaaag
      961 taccgctcaa acgcggctgc gattatcagg atgtgctgaa tatgctgctg ctctatgcca
     1021 gtcctaaggc gtcgtactgc accggacagt cgatcaatgt caccggcggt caggtgatgt
     1081 tctaatcaac agcggagatc cgttaaggat ctccgtgaga ctaatagaat gcctgatgcg
     1141 ctacgctcat caggcataca ggacttccgc cactacatta aggaaaagtt atggtatccg
     1201 cactcatcac cgtcgccgtt atcgcctggt gtgcgcaact ggccttaggc ggctggcaaa
     1261 tttctcgttt taaccgtgcc ttcgacacac tatgccagca agggcgggtt ggcgtgggcc
     1321 gttccagcgg gcgctttaaa ccacgggtcg tggtcgccat cgccctggac gatcagcagc
     1381 gcatcgtcga caccttgttt atgaaaggac tgaccgtctt cgcacgaccg caaaaaattc
     1441 ccgcaattac cggtatgcat gcgggtgatt tacagcccga tgtgatcttt ccccatgatc
     1501 cactatcaca gaatgctcta tcattggcgc ttaaactgaa acgtggataa tttcgttgtg
     1561 aatgttactt gcttgcgaag ttatcatttt gaaacctaaa tcaggtaatc acgcccatga
     1621 aacctcgtca gcgtcaggcc gccattctgg agtatctgca aaagcagggt aaatgctcgg
     1681 ttgaagaatt ggcgcaatac tttgacacca caggcacaac cattcgcaaa gatctggtca
     1741 ttctggaaca tgcaggaacc gtcattcgta cttatggcgg agtggtgttg aataaagagg
     1801 aatccgatcc gcctatcgat cataaaacac tcatcaacac ccacaagaaa gagctgatag
     1861 cagaagctgc cgttagtttt attcatgatg gcgattcgat cattcttgat gctggcagta
     1921 ccgttttgca gatggttccc ctgctctcgc gctttaataa catcacggtg atgaccaaca
     1981 gtctgcatat cgtcaatgcg ctatctgaac tggataacga acaaactatc ctgatgccag
     2041 gcggaacgtt tcgtaaaaaa tcggcctcat ttcacggaca gctggcagag aatgccttcg
     2101 aacatttcag tttcgataaa ttgtttatgg gcaccgacgg catcgatctc aatgcgggcg
     2161 taaccacctt taacgaggtt tataccgtca gtaaggcaat gtgcaatgcc gcgcgcgaag
     2221 tgattttgat ggcggactca tcaaagtttg gccgtaaaag ccccaacgta gtttgcagtc
     2281 ttgaaagcgt cgataagctg attaccgacg caggtatcga tccggcgttt cgtcaggcgc
     2341 tggaagagaa agggatcgat gtgatcataa ccggagagag caatgagtga agcactactg
     2401 aacgcgggac gtcagacgtt aatgctggag ttgcaggaag caagccgttt accggaacgt
     2461 ctgggcgatg attttgttcg cgccgccaat atcatcctgc actgcgaagg caaagtggtg
     2521 gtttcgggaa ttggcaaatc gggccacatt ggtaagaaaa tcgccgcaac acttgccagt
     2581 accggcacat cggctttttt tgtccatccg gcagaagcac tgcacggcga tctggggatg
     2641 attgaaagcc gcgatgtgat gctgtttatc tcttactccg gtggcgcgaa ggaactggat
     2701 ctgattattc cgcgtctgga agataaatct atcgcgctgc tggcgatgac cggcaaaccg
     2761 acgtcaccgc tgggcctggc ggcaaaagcg gtgctggata tctccgtaga acgcgaagcc
     2821 tgcccgatgc accttgcgcc gacctccagc accgtcaata ccctgatgat gggtgacgcg
     2881 ctggcgatgg cggtcatgca ggcgcgcgga tttaatgaag aagattttgc ccgctcccac
     2941 ccagccgggg cactgggcgc tcgcttgctg aataaagtgc atcatctgat gcgccgtgac
     3001 gatgccatcc cacaggtggc gttaaccgcc agcgtgatgg atgcgatgct ggaactcagc
     3061 cgcaccggtc tggggctggt ggcggtatgt gacgctcaac aacaggtaca aggcgtcttt
     3121 accgacggcg atttacgtcg ctggctggta ggcggcggcg cactcaccac gccagttaat
     3181 gaagcgatga cgacaggcgg caccacgtta caggcgcaaa gtcgcgccat cgacgccaaa
     3241 gagatcctga tgaagcgcaa aatcactgcc gcaccggtgg tggatgaaaa cggcaaactc
     3301 accggcgcaa ttaacctgca ggatttctat caggccggga ttatttaatc cttcattccc
     3361 agacgtttcg ccagccgatg caggttggcg acgtcggttt ccagcatccg cgcgcaggca
     3421 gcccagttat gatgattttg tgccagtgcc tggcgaatag tttcacgctg gaacgcttct
     3481 gtcgcttcac gcaggttttg cttaacaacg ggcaccgccg ccgcttctgg cggcggcaac
     3541 gtcacctcag gaaaagcaaa atgttgcgcc tcaagaatca cttcatcgcc gttgcgggtg
     3601 gctctcgcca gcactaccgc ccgatgaata gcatgttcca gttcgcgcac gttccccgga
     3661 aaacgatagt gttgcagtaa atttcgcgct ccggcactta ataccacgcg ggagagcccc
     3721 agccgcaaac gacactgctc gcagaaatac cccgccagca gaatgacatc atcgccccgc
     3781 tcacgcagcg gcggcaccga aagtggaaac acgctcaggc gatgaaacaa gtcagcgcga
     3841 aatcgccctg ccatcacctc ttcgcgtaag tcgcggttag tcgccgccag cacgcgcaca
     3901 tcgacccgca aactacggtc atcgccaacg cgctgaatat cgccatactg caacaccctc
     3961 agcagcttgg cctgcaatgc caacgacaac tcgccgatct catccagaaa cagagtgccg
     4021 ttatccgcca tttcaaactt cccgctgcgg ttactgatag cgccagtaaa cgctcctttc
     4081 acatgcccga acaactcact ttccgccaca ctttccggca gtgcagcaca gttgagatag
     4141 accagcggat tcaccgcccg tggcgaggct tcatgaatcg ctttcgccac cagctcctta
     4201 ccggttcccg tctcaccgct gatcaggacg ttgagatcgg acgccgccac aatctcaatc
     4261 tcttttttca gttgcgtcat gccaggggac aagccaatca tctgcgtctg tttcaccgct
     4321 tcaaacggcg tggcatcgcc tggcatcata ttctggcttt ccagttgttc aatcagcaac
     4381 gcattgctta acgctcccgc cgccagcgca gcaatcagcc gtagctcttc gtcgctgaaa
     4441 acatcgaact gatcgggctg catcccgtcg agcgtcagtg cgccgatcag gttttgcccg
     4501 gcaaacaatg gcagaccaac gcaggcgtga accttcagac tctcctgccc aggaatcaaa
     4561 ccgtcatagg gatcgggcaa ttcgctgtct gcgggaaagc gcaccacatc cccggcgcgg
     4621 gcaatcgctt ccagccgtgg atgcccttcc agcgcaaagc gtctaccgag tacatccttt
     4681 gccagaccgt cgatggcaag cggaataaac tgccgcgaat cgtaacgtag caacgcagac
     4741 gcatcgcact ccagcacctg acgtagcgtg gtgatcaggc gctgaaaacg atcctgatga
     4801 ccaatcccac gctgcaattc gatggcgata ttcgccagca catcaacgga aaaactcatc
     4861 tttgcctcac tgtcaatttg actatagata ttgtcatatc gaccatttga ttgatagtca
     4921 ttttgactac tcattaatga gcataatttt atttatagag taaaaacaat cagataaaaa
     4981 actggcacgt aatctgcaat tagcaagaca tctttttaga acacgctgaa taaattgagg
     5041 ttgctatgtc tattgtggtg aaaaataaca ttcattgggt tggtcaacgt gactgggaag
     5101 tgcgtgattt tcacggtacg gaatataaaa cgctacgcgg cagcagttac aacagctacc
     5161 tcatccgcga agaaaaaaac gtgctgatcg acaccgtcga ccataaattc agccgcgaat
     5221 ttgtgcagaa cctgcgtaat gaaatcgatc tggcggatat cgattacatc gtgattaacc
     5281 atgcagaaga ggaccacgct ggggcgctga ccgaactgat ggcgcaaatt cccgatacgc
     5341 cgatttactg tactgccaac gctatcgact cgataaatgg tcatcatcat catccggagt
     5401 ggaattttaa tgtggtgaaa actggtgaca cgctggatat cggcaacggc aaacagctca
     5461 tttttgtcga aacgccaatg ctgcactggc cggacagcat gatgacttac ctgacaggcg
     5521 acgcggtgct gttcagtaac gatgctttcg gtcagcacta ctgcgacgag catctgttca
     5581 acgatgaagt ggatcagacg gagcttttcg agcagtgcca gcgttactac gccaatatcc
     5641 tgacgccgtt cagccgcctg gtaacaccga aaattaccga gatcctgggc tttaacttac
     5701 cagtcgatat gatagcgacc tctcacggcg tggtatggcg cgataacccg acgcaaattg
     5761 tcgagctgta cctgaaatgg gcggctgatt atcaggaaga cagaatcacc attttctacg
     5821 acaccatgtc gaataacacc cgcatgatgg ctgacgctat cgcccagggg attgcggaaa
     5881 ccgacccacg cgtggcggtg aaaattttca acgtcgcccg aagcgataaa aacgaaatcc
     5941 tgaccaatgt cttccgctca aaaggcgtgc tggtcggcac ttcgacgatg aataacgtga
     6001 tgatgccgaa aatcgccggt ctggtggagg agatgaccgg attacgcttc cgtaacaaac
     6061 gcgccagtgc tttcggctct cacggctgga gcggcggtgc ggtggatcgt ctttccacgc
     6121 gcctgcagga tgcgggtttc gaaatgtcgc ttagcctgaa agccaaatgg cgaccagacc
     6181 aggacgctct ggagttatgc cgtgaacacg gtcgcgaaat cgcccgtcag tgggcgctcg
     6241 cgccgctgcc gcagagcacg gtgaatacgg tggttaaaga agaaacctct gccaccacga
     6301 agtccgacct cggcccacgg atgcagtgca gcgtctgcca gtggatttac gatccggcaa
     6361 aaggcgagcc aatgcaggac gttgcgccag gaacgccgtg gagtgaagtt ccggataact
     6421 tcctctgccc ggaatgctct ctcggcaaag acgtctttga cgaactggca tcggaggcaa
     6481 aatgagcaac ggcattgtga tcattggttc gggcttcgcc gcccgccaac tggtgaaaaa
     6541 tattcgcaaa caggacgcca gtattccatt aaccctgatc gccgccgaca gcatggatga
     6601 gtacaacaaa ccggacctca gccatgttat cagccagggg caacgtgccg acgaccttac
     6661 ccgccagacg gcgggtgaat ttgccgggca gtttaatctg cacctgtttc cgcacacctg
     6721 ggtgacggat atcgatgccg aagcccatgt ggtgaaaagc cagaataatc agtggcaata
     6781 cgacaagctg gtgctggcaa ctggtgccag tgcctttgtc ccgccagtgc ctgggcgtga
     6841 gttaatgctg acgttaaata gtcagcaaga gtatcgcgcc tgtgaaacgc aactgcggga
     6901 tgcccgacgc gtgttgattg tcggcggtgg tttgattggt agcgaactgg cgatggattt
     6961 ttgtcgtgca ggcaaagcgg tcacgctaat cgacaacgct gccagtattc tggcgtcatt
     7021 aatgccacca gaagtaagca gccgcttgca gcatcggttg acggagatgg gcgttcatct
     7081 gctgttgaaa tctcagttgc aggggctgga aaaaacggat tctggcattc tggcaacgct
     7141 ggaccaccag cgtagtatcg aagtggatgc ggtaattgcc gccaccggac tgcgcccgga
     7201 aacagccctg gcacgacgcg ccgggctgac gattaatcgc ggcgtttgcg tcgatagtta
     7261 tctgcaaacc agtaatgacg atatttatgc gctgggcgat tgcgcggaaa tcaacggtca
     7321 ggtactgcca ttcctgcagc cgattcaact tagcgcgatg gtgctggcaa aaaatcttct
     7381 cggcaataac acgccgctga aactaccggc gatgctggtg aaaatcaaaa cgccggaatt
     7441 accgctgcat ctggcaggcg aaacccagcg tcaggattta cgctggcaaa ttaataccga
     7501 acgccaggga atggtggctc gcggtgttga cgatgctgac cagcttcgcg cctttgtggt
     7561 cagtgaggat cggatgaaag aggcatttgg attgttgaaa acattgccga tgtaggtggg
     7621 ctactgtgcc taaaatgtcg gatgcgacgc tggcgcgtct tatccgacct acggggacgc
     7681 atgtgtaggc cggataaggc gtttacgccg catccggcaa tggtgtccaa atgcaacacg
     7741 ttttatccgt tctggacttc acccgctaac caacgcgccg cagcaataac cccctgcccc
     7801 agagacaaac cgccatcacc cgccggtaaa ctctgtggaa agagcaatgt gaaatcagcg
     7861 agataatgcg ccagacgtgc acgcagcaaa cggttatgaa taaccccgcc gctaaatacc
     7921 agcgtagtga taccacgcat cgtggcctgc tcacgcatca acgcggcaaa accctgcgcc
     7981 agcgcatcat gaaacgccca cgcgcgttga ttaaccggtg cctgccagtt cagccactgc
     8041 tgccagaaag cggcgagatc cagttgattg tccaccagcg gcatcgtcac cggatgcgtc
     8101 actccgtggc atgaggctgc gagcgcctcc agagcacaag ccgcttcacc ttcataactt
     8161 aacgtggctg gcgcacagcc cagtgccgcc gccactgcat cgaacaaccg cccacacgat
     8221 gacgccagcg gcgcgttaat tccacgctca atggcccgcg ccagcacgct ccagttttgc
     8281 tgttgcacac ttgctgtttc agggtaattc tgccactccg gcacaaagcg caggcactgc
     8341 gccagcaggt ttcgccacgg ctgcttcgct gccaaatcgc cacccggaag cgccactgca
     8401 ggcaagccgc ccaggtgctc acattcgcga tagttcaccc gcaggcactc gccgccccac
     8461 aaagcgccgt tctcccccat accgataccg tcgagcgtca aagcaatgac atcaccgcca
     8521 tccaacggcc actgatgctc tgccagacac gccgctgcat gggcatgatg atgcagtacc
     8581 gtttgcgtcg gcagattcat ttcacgcgcc cactggctgg agacatagcc cggatgcgcg
     8641 tcatgcacaa cgtattgcgg ggtaaaatcg tagatgtttt gcatcaggcg taacgcttcg
     8701 cgccactgca tctggatgcc atcgtcactt aaatcgccca gatgctgact caacaccgct
     8761 tgttcaccgc gcaccaggca gaaggtattt ttcagatccg cgccgagaca cagcacaggc
     8821 ggaacatttt taaagcccgg aggcaaagcc agcgcatccg gcacataccc ccgcgaacgg
     8881 cgcagcattt cgccgctttc gcgcaccacc gaatcatcca tccgctgcac gatgtcgcgg
     8941 ttatgtatca agaatccgtc ggcaatgccc tgcaaatccg ccagcgcctg ttcgttgctg
     9001 atagctggtg gtttaccgct caggttgccg gaggtcatca ccagcgggca ttgcagttcc
     9061 tgtaacagca aatgctggag cgggttcgca ggcaacatta ccccgacttc gttaaggtca
     9121 ggggcgatat catcacaaag ctcaggaacg tattttttat ccaccagcac aatcggcgcg
     9181 gcgggcgtgg taagcaactg gcgcgcagcg tctggtaaac cgtcagccac tggcaacatg
     9241 accgccagcg gtttcgccgg gcgatgtttg cgcgcccgaa gtgtcgccac cgcgttactg
     9301 ttacgtgcat cgcaggcaag atgaaatccg ccaatccctt tgatggcgac aatgttgccc
     9361 atttttaact gtgcgatagc cgcctgtaat gccgcctctt gttctgcatg ttcaccatga
     9421 cttacccatt caagatgtgg gccacactcc gggcaggcca ccggctgggc gtggaagcga
     9481 cgatcgagcg ggtcacggta ctctttgtca caagccggac acaacggaaa cgccgccatc
     9541 acggtaaacg ggcggtcgta aggcatggcg cgaataatgg tgaaacgcgg gccgcagtgg
     9601 gtacagttga taaacggata acgataacgc cgttcgcctg gggtattcat ttcggcaagg
     9661 caagcagggc aagtagcggc atccgggaca atttgcgtat tcatggcacc tcccgcgctc
     9721 tggcggatgg tgaactcagt gggcagttgt gaccagatgt acggctcacg ctcgacgcta
     9781 tcaatacgcg ccagcggcgg gcagtgctga tgcaattgaa caagtaacgt ttccgggtct
     9841 tcccgcagcc ggacttctac gccatcgccg tcattacaga catcgccgtg aagatttaat
     9901 tgctgtgcca gctgccagac aaacggacga aaaccgacgc cctgcacttt gccacgaata
     9961 cgcagttgga caccgcaaga tgtgtttttt gccattgagt tattcccgcc atcatgaatt
    10021 gcgtaacccg tcctgccgga cacgactgcg tcgtatccgg cagtctcagg tcggcgatac
    10081 cgccagctcc gtattctacg aatatttccg ggaattcctt tgatgccaga acagttctgt
    10141 aagattttta gaacatcagc gccgtacggc ggcgtttttc tgcactcagt tgttcaagtt
    10201 tattacgatc gacacaaatc agcgcatgag tcgggcaagc cgccatacac gccgggccgt
    10261 cttcacgatg gttgcacagg tcacatttat tggcttcggc tttgtcagcc cgtacattca
    10321 gccccgcgcc gctgttgcga atcaccggac gtaccaccac ttccatcgcg ccatacgggc
    10381 aagccacaac gcaggttttg caaccaatgc aacgttcctg catcacatga acaaaccctt
    10441 tatcacggct gatagcacca ttcgggcaga cgttagcgca cggtgcatct tcacactgac
    10501 ggcaaactgt cgccgtggaa atgttcacac ctttaatgac atggatacgc ggtaaaaatg
    10561 tttccggggt cagcgatgca cagtcctgat tttcctgatg agaaaccacg cacgccactt
    10621 cacaggtacg gcaaccaata catttactcg cgtcagcaat gatgaaacgg ttcatcaaat
    10681 tctccagcaa tgacagttaa tgcgccgata cattcacaaa tcatgccagt tttaattcac
    10741 tgttatttaa ggaaattaat ttctgtaatg caggaaaaaa gatgtcatcg acactagtga
    10801 cgatgacatg tgatgacaat gtttatcgcg aaagagcaat gagtgagtcg cggcggatca
    10861 gttttccgct gaaggttttc ggcggtgaga aatccccgcc atcgagcata aaaatcagcc
    10921 gtccaataat ttcctgaatc atctcagtca ccggaatttt tacgctggag agcgccggaa
    10981 cgatataggg ggcaattgcg atatcatcga atccgataac tgacacctgc tctggcaccg
    11041 ctacgccgcg ctcgtgcaac gctttcatcg cacctatcgc catatcgtcg ttactggcaa
    11101 ctaacgcgct aaatttagcc ccacgttcga gcaacgtttc taccccttcg gccccgctgg
    11161 cgggcgtcca tttaccgtta gcgataagtt tttcattgag cgcaatacca tgctgcgcca
    11221 gcgcgtcttt atacccagca agacgttcaa tgctggtggg ggaatccatc gagccggtaa
    11281 ggaaagcaat ctcctgatgt ccggcgttta tcaactctgc cacggcatta aagctggtct
    11341 gtttatgatc gcaccagacg ctatggctgc tgtttttgcg caggcggcga ttaagcacca
    11401 ttatcggctg actgtgcgcg tcaatgatgt catcgatctc atccacgctt aaaaaacgcg
    11461 ggtaaatcat aatggcgtcg cagcgcaaat ccagcagata ctgaatcgcc tggcgctctt
    11521 cttcggcgct gtgtttacca tctgccaata gcaactgccg ccctttctct tccgccattc
    11581 gcgcggcatg aaagagtaat tcactaaaat aaatgccgtg gtaaagcgtg ttggtcacta
    11641 ccaaccccag cgtctgagta ctcttcgccg acagattgcg cgccagcaag tttgggcggt
    11701 aaccgctctc ttctaccgcc tgaaacacgc gatctttagt ctcctggctg acgtagccat
    11761 tacctgaaag cacgcgggaa acggtcgctt ttgaaacccc ggcgcgcttc gccacttcca
    11821 gcatcgtcgt catcattttc atccctttac acgcaatcaa cgcagtgtac tgcaccgttt
    11881 gccgattgtc cttgcgcaat cggcgggaaa aatattcagg tgaccggttt cacaaatata
    11941 aaaaatgaac aattaactct cttgcttatt tagtgacaac tattcatgat tttgtgaaac
    12001 cggtttccta attccgtttc agcatcggca tttttccgtt acgtcgactg ataacaacta
    12061 catctaccct actgataaca ggataaaatc cgatggccaa aaattatgcg gcgctggcac
    12121 gctcggtgat agcggcactg ggcggcgttg ataacatctc ggcggtcacg cactgtatga
    12181 cgcgtttgcg cgttgttatc aaagatgacc agcttatcga cagcccgacg ttaaaaacca
    12241 tccccggcgt gctcggcgtg gtacgtagtg acaaccagtg tcaggtgatt atcggcaata
    12301 ccgtttcaca agcctttcag gaagtcgtca gcctgctgcc gggagatatg cagcccgcac
    12361 agcccgtggg taaacccaaa ctcacgctgc gtcgcattgg tgcggggatc ctcgatgcgc
    12421 tgatcggcac catgtcaccg ctgatcccgg cgattatcgg cggatcgatg gtcaaactgc
    12481 tggcgatgat cctcgagatg agcggcgtgc tgacaaaagg atcgccgacc ttaaccattc
    12541 tgaatgtgat tggtgacggt gctttcttct tcctgccgct gatggtcgcg gcatctgccg
    12601 ccatcaaatt taaaaccaat atgtcgctgg cgattgccat cgcgggtgtg ctggtacatc
    12661 cgagttttat tgaactgatg gcgaaagcgg cccagggtga acatgtcgaa tttgccctga
    12721 ttccggtcac cgcggtgaaa tacacctaca cagtgatccc ggcgctggtg atgacctggt
    12781 gcctgtcata tatcgaacgc tgggtggatc gcattacacc ggcggtgacc aaaaacttcc
    12841 tcaagccaat gctgattgtg ttgattgccg caccgctggc aatcctgctg attggcccga
    12901 ttggtatctg gatctgtagc gccatttcgg cgctggttta caccattcat ggttatctgg
    12961 gctggctttc agtcgccatt atgggcgcgc tgtggcctct gctggtaatg accgggatgc
    13021 accgcgtctt tacgccaacc atcattcaga ccattgccga aaccggcaaa gaagggatgg
    13081 tcatgccgtc agagatcggc gctaacctgt cgctgggcgg ttcatcactg gcggtggcgt
    13141 ggaaaacgaa aaacccggaa ctgcgccaga cggcgctggc tgcggcggca tcagccatta
    13201 tggcggggat ttccgaaccg gcgttatacg gcgtggcgat ccgcctgaaa cgtccgctta
    13261 tcgccagtct tatcagcggt tttatttgcg gcgcggttgc cggtatggcg gggcttgcca
    13321 gccactcaat ggcagcgcca gggttattta ccagcgtgca attcttcgat ccggcgaatc
    13381 caatgagcat cgtctgggtg ttcgcggtca tggcgctggc ggtggtgctg tcgtttatcc
    13441 tcacactgtt gctcggcttt gaggatattc ctgttgagga agcggctgcc gaggcgcgaa
    13501 agcatcagag cgtacaaccg accgtcgcca aagaagtaag tcttaattga ggatgaaaat
    13561 gtcagtattt ccagaaggtt ttttatgggg cggcgcgctt gccgccaacc agtctgaagg
    13621 tgcgttccgt gaaggtggca aagggctgac cactgtcgat atgatcccac acggcgagca
    13681 tcgaatggcg gtgaaactgg ggctggaaaa acgttttcag ttgcgcgatg acgagtttta
    13741 tcccagccat gaggcgacgg atttttatca tcgttataaa gaagatatcg ccctgatggc
    13801 agagatggga ttcaaggttt tccgtacctc aattgcctgg agccgtctct ttccgcaggg
    13861 cgatgaaatc acgcccaatc agcagggcat tgctttttat cgttctgtct ttgaagagtg
    13921 taaaaagtac ggtatcgaac cgctggtcac gttgtgccac ttcgatgtgc cgatgcatct
    13981 ggtcaccgaa tatggctcct ggcgtaaccg caagctggtg gagtttttca gccgctacgc
    14041 ccggacctgc tttgaagcat ttgatggtct ggtgaaatac tggcttacct tcaatgaaat
    14101 caacattatg ttgcatagcc cgttctccgg cgcgggtctg gtgtttgaag aaggtgaaaa
    14161 tcaggatcag gtgaaatatc aggccgcgca tcaccagctg gttgccagtg cgctagccac
    14221 caaaatcgcc catgaggtta acccgcaaaa tcaggtgggc tgtatgctgg cgggcggtaa
    14281 cttctaccct tacagttgca agccggaaga tgtctgggcg gcgctggaga aagaccggga
    14341 aaacctgttt tttatcgatg tgcaggcgcg gggcacgtat ccggcttact ctgcccgcgt
    14401 attccgcgaa aaaggggtaa ccatcgacaa agcaccgggc gatgatgaaa tcctgaaaaa
    14461 caccgtcgat tttgtctctt tcagctatta cgcctcgcgc tgcgcctcgg cggagatgaa
    14521 cgccaacaat agcagtgcgg cgaacgtagt gaaatcgctg cgtaacccgt atctgcaagt
    14581 gagcgactgg ggctggggta ttgatccact cggtctgcgt atcaccatga acatgatgta
    14641 cgaccgttat cagaagccgc tgtttctggt ggaaaacggc ctcggcgcaa aagatgaact
    14701 tgctgccaat ggcgagatta acgatgacta tcgcatcagc tatttacgcg aacatatccg
    14761 cgcaatgggc gaagcgattg cagatggcat tccgctgatg ggctacacca cctggggctg
    14821 tattgattta gtctccgcct ctacgggtga aatgagcaaa cgctacggtt ttgtctacgt
    14881 agaccgtgac gacgcaggca acggcacgct gacgcgcaca cgtaagaaat cgttctggtg
    14941 gtataaaaaa gtgattgcca gtaatgggga agatttagag taggtaacag tgccggatgc
    15001 ggcgtgaacg ccttatccgg cctacatgtc gcacgaacca ggaaggcccg ataagcgcag
    15061 cgcatcgggc aatctggggt ttggcatcag tcaaaatcaa agaaaccatt ccggcttaac
    15121 gccaaatcgg gctgaaagtg ccttaatgtg ggatatcgtc agggatcgtt gtccagacaa
    15181 aatctgactg accagggatt tagagccgat ttcgttcttc aaatccgcat aggtcagatt
    15241 atgttgatca atcagcgtgc gaagtaacgc cacgccaaca ggcattgccg caacggcttt
    15301 gtcgaactct gcaaactctt ggttgttgtt ttcatattct gcaatgcggc tcgccagaaa
    15361 gtcgataagt ggattggtgt catcttcttc aatcaaatat tcgaccagtt ccagtgcttc
    15421 acgataatca tcttctgagt cactacctcc cagaaagggg actgcattga ccagttctct
    15481 tgttgcttta acagcgcgtg cagcattagc agtcattctt tgttctctct gtagtaacgt
    15541 gtcagtttgt catactcggc gtgattagtt atgtgcttca caaagaaccg cttattaaca
    15601 aagtttatgt aggcaatcac ccttaggttg ttcccaccta catccagaac gtaccatttg
    15661 ttgcgatatt taaaattgtc gaggttggga aacgccgttc gcatctcttc tggcgtagaa
    15721 aaatccgttt ccttaataac ccgatacaat gcctgaagag ccagagcgtc gttaggatat
    15781 ttacgcgcag actcttcaaa tggcgcttta gaaatgatgt gcatttttaa gttttaactt
    15841 ccttatagta agtaaacagc cacatctgcg agttctatcc ttatgtgttc ggaaactata
    15901 gctcatcaaa tccaagttca caatatgtaa acacttgagc gcgatataat tacccccaaa
    15961 atcctcatag ccactctgca aaaagtcagt tctggttcac cccatcaaga acatccccct
    16021 ttatcaagca acgctgcgtc tgcgccagat cttccgtttg cgggtcgcgc ccggcgatgg
    16081 gtttgaggac gagtttacgg ttgggggtat aagtaaaacg cgtgagcgcg ggatagctta
    16141 acttgccaaa ccagccgtga acacgctgcc atttggcgtc ttttacctga tacagactgg
    16201 ttatccagta accggaatca aactgcatat ccagcaattg cgtgtgacca ttgccttgca
    16261 aatccagcaa atcatctacg ccagttttgc ttgcggtgta gaatcccctg ggttcaaaga
    16321 cacagggacg gccttcgcgc gtgaacgtca taagaattaa atgtgcataa ggagccagac
    16381 cattaccggt acttggtaac cagataacca gatcctgaat gccgttacga tcaagatcgg
    16441 cgcgatagag ttgccctttg cccgctatgt ttgccagcat acccacaggc acagaccagg
    16501 atttaccggc gcgatcttta ccgttcaatt gccattcact atcatcattt ggcatctttt
    16561 caagcgtcac gccagcaacg cccacttttc taaaatgcca ggtttgctcg gcatcataag
    16621 gcatggtcag gagaggtttc agcgcccgct tttgcgcgta ttcgctgtca ctcattggca
    16681 gatattcggt aaagctggtg gcctgtgcag gcagcactaa gctcaataaa aaagcgcaca
    16741 tccctgttct gatattcatt tcgctgaatt ccctgatgga gaattattct tcttccaccg
    16801 ctaactgcgc gaagccgcca ttcccttccc agccttccag tcgttgataa acggtttcta
    16861 ccgcatcttt aatcggctga gtcatcgggt agtaaaagcc gacgatatcc ggctggatgc
    16921 cgaggaaaat cacttcgcca atgtcttctt tcaactggtc gataagatag ttcagcggca
    16981 tgttatgggt agtcatcata aacatctcgg cgatatcatc cgggtcgatg atgcggattt
    17041 cgccggggtt tagccccata tcagtagcgt caacaatcag taaccgcgta gggcgcagtt
    17101 cacggatggc gacgatgtcg ttttccggtg cactaccgcc atcaatcacc acccagttac
    17161 ctttcggcgc ggcggcgcac ttttccgcca gcagcgggcc agcgccatca tcgcccatca
    17221 tgctattgcc aacacagagt aaaacgtcag tcacgcagtc tcctcaccat caaatagatg
    17281 gcgttttcct gatgaatatc atgcagcata cttaacagca ttgtactcca ttccttctgc
    17341 tgcgaagttt gcgctgcccg ggctttatcg aatgcgttgg ccagcatcgg cacatggtta
    17401 atgtcgatga cgatctcacc atatttcggc acgccttcca ttttgcggcg ggcttcactg
    17461 cccgcctcca gagtggcaat ccatgccaga tattcatccc acgggcaggt gagcgccgct
    17521 tccaggcaat cgataacccc aaggtggtga ccaatcgcca ggctgtaata gaccacctgc
    17581 tgcgcctcgg cgggcgtggc atcgttctca tcaataaatt tacggctcag ttgactgaac
    17641 accacctttt cactcatcgg atacgcgcct cttcaacaac atgattcaga tggctgacaa
    17701 tctcgttcag acgcggatcg ttttccgctt ccagccagcg tgcaacctgt tcttcgccct
    17761 gccctaactg tgtcaggaag tcatcggcaa tctgacgacc gtaacgataa cccgccaggc
    17821 ggcgtgcttc gcgatccact ttcacacgca gcggctgcac catatcgcca tgcaggattt
    17881 ccgcctgttg ttcatcctgc tcacccgggc cacgggcgtg aattttctgc tccagcaggc
    17941 cgagcgccat tgcaaagccg tacagcgtgg cggcaggcgt tggcgggcaa ccaggaatgt
    18001 agacatctac cgggacgatt ttatccgtac cgccccacac gcagtagaga tcgtggaaaa
    18061 taccaccgct gttaccgcag gcaccgtagg agatacaaat tttcgggtcc ggcgcggact
    18121 gccacgcacg cagcgcaggg gatcgcattg cacgggtgac cgcgccggta aacagtaaaa
    18181 tatccgcatg acgcggtgaa ggaacgactt taatgccgaa gcgttctgca tcaaacagcg
    18241 gcgaaagcgt ggcgaaaatt tcgatttcgc agccgttgca gccgccgcag tccacgcgat
    18301 aaacgtaggc agaacgtttg atttttttca gtaacgacgc cttcatgctg gcgatggatt
    18361 catccaccgt catcgggacc ggaatgccgt tggcgtcacg ggggcctaat aaattgctca
    18421 tcagatggcc tctttcatat ggcgagtcag ttcaatacgg tcggacggca ccaggcattt
    18481 ctggcgctta cattccgggc aagtctcaaa gctttcgcgg tggttttccg cgcggctgtc
    18541 gccgttgtgc ttaagcagcg caatggcgta gtcgatctct ttctggacgg cgaaaggacg
    18601 attgcagacg cggcagttgc acagcgcgaa gcgggactgt tgcaggaagt cttctttctt
    18661 ccacactgcc agttcgtact cttgcgacag tttgatcgcc gccgtcgggc aaacttcttc
    18721 gcagcgtcca cagaagatgc agcgcccgag attaaactcc cacgccagct caccggtggc
    18781 gaggtcagtt tcaaccgtta aggcgtttga cgggcaggca ttgacgcagg ccgcacagcc
    18841 gatgcactgc tgcgggttct gctccggctt accacggaag tttttatcaa ccgcaatcgg
    18901 ctccagcgga taagacgagg tcgccgtgcc ggttttgatg acttttttga taaaggtaaa
    18961 catggcgatt ccttatttca gcggcgagtt tttacgctca atgctgtaac gctcgagttc
    19021 tttgtacggc accactttgc tctttttctt acgcacatcg accacggtca tgcggtcggt
    19081 acaggagtag caagggtcca agctaccgat aatcagcggc gcatcggaaa cggtgttacc
    19141 gcgcagcatg tagcgcaggg tcggccagtt cgcgtaggtc gcggcacggc agcgccagcg
    19201 gtacagcttc tggttgtcgc cggtcatgct ccagtggata tcgtcgccgc gcggcgcttc
    19261 ggcaaagccc agcgcgaaac ggtgcggaat gtaggtaaag ccttccacca tcagcggacc
    19321 gcccggcagg ttatccagac cgtagtcgat catgttcagc gcggtataaa cttcgttgat
    19381 acgcactttc agacgggaaa taacgtcgca gccctgctcg ctgtggactt ccattggcag
    19441 caggccataa ccgacaaacg ggtgatcggc gcgagtatcg cgggcgtgac cgctggcgcg
    19501 aaccatcggg ccaacgttac tgaaatcgcg ggccatttcc gggtccagac gaccaatgcc
    19561 gacggtgcgc tgttccatgt tcggcgtact cagcagcaca tccaccagct cctgcacttc
    19621 acgacgcatc tgttgcgcca gctggcgggt ctggatcatg tcatctttca acagatcgcg
    19681 acgaatcccg ccgatcaggt tcaggccgta ggttttacgc gcaccggtga ggatctctgc
    19741 cattttcatg gaggtttcac gcacgcggaa gaactgcata aagccggagt caaagccggt
    19801 aaagtggcag gcgagaccga ggttgagcag atgcgagtgc aggcgttcca cctccagcag
    19861 aatggcgcgg atcatctgcg cacgttctgg cacctgaata cccatcgcgt tttctaccga
    19921 cgtggtgtag gcggtgctgt gggcaaagcc gcagatcccg cacacacggt cagagaggaa
    19981 ggtcacttcg ttgtaaccca tacgggtttc cgccagtttt tccatgccgc gatgaacgta
    20041 gaacagacgg tagtcggcgt cgataatgtt ttcgccatcg acgaacagac ggaagtggcc
    20101 cggttcgtca gaagtgacgt gcagcggacc aatcggcacg acgttgtttt tcttgtcgcc
    20161 cagttcgttg atgaactcgt aggtttcagc atcggtggtc ggtgccggac gctgacgata
    20221 atccatgctg tctttacgca gcggataaag ttcatccggc agcaccagac ggcgttcatc
    20281 cggcagacca accggaatca aaccgtacat atcgcgcact tcgcgctcgc cccacaccgc
    20341 cgccggaacg cgcggcgtca cggacggata ctccggtttg ttggcatcaa cttcgacgcg
    20401 aaccgttacc cagcacttag tccccttctc catcgacagc acgtaataaa cggcgtaatg
    20461 accattcagt ttgcgttcgt cgttaccaaa aagcaccgac aaccagcccc cctgcttgta
    20521 gtagagaaac tccaccactt ccggcagata gttcaccttt acggtgacag tcagctgatc
    20581 tttggtctgc caggcgtggt ccagcacgac gcccggaaat gcctcattca gcgcggcgag
    20641 ataatgttga cctaattttt cttcagacat gctcaaactc tctttaatca cgccgccagc
    20701 aaggagacga acgctaaaaa tgcaaagcca aacccagtcc aggtaatgcg cggagtaata
    20761 tcaagacgca gacgggccat gctgttttcg aacagcgcga taaccaggac gccgacgacc
    20821 agtttgacga tggcaatcac cagcgccagc agcagcccac cggcggtgaa ggtttccatc
    20881 tgtccccacg ggataaatac gccgacgaac atctgcagca ccaccagctg tttcaggctg
    20941 ataccccatt tcatgacgcc aaagccgctg ccgctgtatt cagagagcgg gccttcctgc
    21001 agctcctgct cggcttccgc caggtcgaac ggcagtttgc ccatttcgat aaaggtggcg
    21061 aacgcacagg cacaaagcgc cagtaccagc gggatgctct ggctcagcgg ccagtgataa
    21121 acggtgtcgg tgatgttgct gatgttggtg gaaccggcaa cctgtgcggc aacccacaga
    21181 ccaagcagca gcatcggttc gaccagcacg ccaagcatcg cttcacggct cgcgccgata
    21241 gcggtaaacg ggctaccggt atccagacca gaaatggcaa agaagaaacg cgcgatggca
    21301 aagagataca gtaaggtgat taaatcaccc agttgcggca gcggagaacc gacggtcacc
    21361 accggcagcg cagtagcgat agtcagcatg acgcccacca tcacatacgg cgtcaggcgg
    21421 aacacccagc cggaggcatc cgggccaacg ctctggcggc ccagcagttt gaaaatgtca
    21481 cgatactcct gcaacacacc cggcccacga cggttatgca gacgggcgcg cgccacgcgg
    21541 gtaataccgg agagcagcgg cgcaacggca aataacacca gcgcctgaat taacggatat
    21601 aaaacactca ttctcaggct cctcgtgaaa caataatcac caccagtacc gccagttcaa
    21661 ccagcgccat ccggcggaac agcaacgcac taccttcgca ctgccagccc ggcaccagcg
    21721 acaccggatt cagccatttg cgcagtttca gcactggcgc aaacgcctgt ttaaccggca
    21781 tggcaaaacc gtgagcggta atcaccattg atttttcatg gtcgtagccg cacacccagg
    21841 ccgcaccgcg ggaacgtgac ggcaggcggt cgcctttgca aatcgccata atgatgaatg
    21901 gcagcagcgg gcaggcaatc agcagcaatg tgatcattgg ttgagaaacg gtggtgttag
    21961 caggctccag aggcagaggt acagcagcag agagcatcgg cagcagccac ggcgcagcaa
    22021 caccgccaat tacgcagcaa atcgccagtg ccactacgct tacgctcatc aggagcggcg
    22081 cacaggtggc gttttcggct tctttggtac gcggcgcgcc gaggaacgtg acgccataga
    22141 ctttcgccat acacatcacc gccagcgcac cggtaattgc cagacccaca gcgagcagcg
    22201 gccccagcag acgggcaaca aacgcgccgc tattgctcag tttgaagaag gattgataga
    22261 taacccattc cccggcaaac ccgttcagtg gcggcagcgc agccattgcc atcaacccga
    22321 ctaacatggc gatggagata accggcattt tcttgccaat accaccgagt ttttcgatat
    22381 cgcgatgacc ggtacggaac cagacgctcc ccgccccgag gaacagtacg cttttgaaca
    22441 ggctatggtt aagcagatgg tacagaccac cgaccaggcc aagagcaatc agcgccggtt
    22501 gttcgagcgc gatacccgtt acgccagcgc ccagccccag caggataatg ccgatatttt
    22561 ccagggtgtg gtaagccagc aggcgctgga tgttgtgttc catcaacgca tacagaccac
    22621 cgacgaacgc ggtgatcatg ccgagcacca gcagcgcgat cccccaccac agcggtgcat
    22681 taccgcccag cagtgacagg gttaacatgc ccagcaggcc aattttcatg actaccgtag
    22741 aaaacaacgc ggcagctggc gcagaggcgt tagcatgtgc ctgcggcacc cagccgtgca
    22801 gcggaataat cccggccagc aggccaaagc caatcacacc gagcagccag atatcggaac
    22861 cgagcggcag ctgttgcata cgcatatcca gtaggcacag atccagcgtg ccataacgct
    22921 gccacagcag ccagcaagca atcaccagca gcagagtgcc aagacgcccc agcgcaaacc
    22981 acagtttgcc ctctttgctg ttgctggtga ggaacaccgc acacaaggcc atgacttcgg
    23041 ccattaccac gaacatgccg aggttgctgg cgatgacggc gcagacggcg gcagccatca
    23101 acatattgat ctgcaagccg ttgcacttca cctgcgcgtg gcgatgccag tcaatgttgt
    23161 agaggctgac aaacagaccg cacagaccga gcgtaatcag ccaaatcgcg ttaagcggag
    23221 agatttgcac atcgtagctt accagcgaca gcgcaccgct cacgccaacc gcgccagtca
    23281 gtacagtgaa gcccgcggct gccgtataca gactaccaac cgcgccgcca attccagcta
    23341 tccagccact taacgctttt tgaaaagaaa agagaaatgc cagaacagcg gcggcgacaa
    23401 accatgccac gccgctattg atcagggaaa ttgcgctcat ttagcctctc cactttgagc
    23461 ctgctgaaac aaggtgagat cgccaaagtc cgtgttaaag gtcagctcac gcttacgttt
    23521 gctgacgcgg gcgatatcgg tgttatccac cagatgcagg gctttagtcg ggcacatccg
    23581 cacgcaggcc ggaccttgtt catcaaaact gcaaaggtca catttgacgg cgatcgcgcg
    23641 aatacccggc acccagtcaa gcagcgtact gatacgcgcc ggagcaggcg gtgccggtgg
    23701 cgctttcggg gtattggcgt ttgccggaat atccagcgga cggctgccgg aaaattcaat
    23761 tgcgccaaac gggcaggcga tgccgcacag tttgcagctt acgcacagac tttcattcaa
    23821 ctgcacggcc ccatcaatgc gggtgatggc gttaaccggg caaaccaccg cacagggtgc
    23881 atcttcacag tggtgacaga gctgcggcgc agattctttt tcattcagca tcactctcag
    23941 gcgcggcatt gattgcaggc cgtgctggcg atgcgtctct gaacaggcgg cctcacaagt
    24001 gtggcagccg atacagagcg tggagtcaac aattacaaaa cgattcacca ggcattcctc
    24061 aggtgattgt catttttgac gaaaacatgc cgatgaaatg tcattttcga cactcatcga
    24121 cacgcccatc cccaaacagg cgtaacgcct gcaaaacggg caaagcctca gctcatgctg
    24181 ccgggctttg tccctttacc agttggctta aattcaccgg cacattgttt tcaatagcgg
    24241 tgtataagct gtcgtaaacg ccagcgattt tttcgagata gtgttccaga acctgttcgc
    24301 gatcgcggat gttgaccttg ccatcaatca tgccgtcaat gctcagttgc gcctgcgcaa
    24361 tccgctgttt gtcttcgcca tcttttgttt cgacgtaata ctcgatggtg tggctgttaa
    24421 agccatccgc cagggtgacg taaatacgaa aatgttcaaa aaggacgaaa cagagttctt
    24481 tgtccggcgc gcagctcatg gcgtgatgca ggcgcgcttt cgataacgtg atcccctgaa
    24541 ccagcgaatt gcagtagatg tgccactggt cctgtaggcg acgatgccgc tgtgcgatgt
    24601 aatcggcttt ctcgcttatt tcccaaatag tcattgtcag gttacccgtt taacagagat
    24661 gccagcttta agcattttct atgccaactt ttaatttatt gttattaaat agatttttaa
    24721 gctaaagatg aatttcgtcg ccgtgtcgac gtgtcatttc gacatcatcg acattattca
    24781 ccgcagggat aatcaacact ggcacaatta ttgcttgtag ctggcaatag ttaatgggag
    24841 gcgatatgca cgaaataacc ctctgctaac gggcactgga attgatcgaa cagcaggccg
    24901 caaaacacgg cgcaaaacgc gtaactgggg tctggctcaa aattggcgca ttttcttgtg
    24961 tcgaaaccag ctctcttgcc ttttgttttg atctggtttg ccgcggcagc gtggcggaag
    25021 gttgtaaact gcacctcgaa gaacaagagg ccgaatgctg gtgtgaaaca tgccaacagt
    25081 atgtgacgct actgacccag cgcgtccgcc gctgtccaca gtgtcatggt gacatgctac
    25141 agattgtggc agacgacggt ttacagattc ggcggataga aatagaccag gagtgagcga
    25201 tgtgtacaac atgcggttgc ggtgaaggca acctgtatat cgagggtgat gaacataacc
    25261 ctcattccgc gtttcgtagc gcgccatttg ccccggcggc acgcccgaag atgaaaatca
    25321 ccggcattaa agcgcctgaa tttaccccca gccagactga agaaggcgac ctgcattacg
    25381 gtcatggcga agcgggcact cacgcgccgg gcatgagcca gcgtcggatg ctggaagtcg
    25441 aaattgacgt gctggacaaa aataaccgtc tggctgaacg caaccgcgcg cgctttgctg
    25501 cccgcaagca actggtgctc aacctggttt ccagccctgg ttccggtaaa accaccctgc
    25561 tgacggaaac cctaatgcgc ctgaaagaca gcgttccgtg cgcggttatt gaaggtgacc
    25621 agcaaaccgt gaacgatgcc gcacgcattc gcgctaccgg cacaccagcg attcaggtga
    25681 acaccggtaa aggctgccat cttgacgcac agatgattgc cgacgccgca ccgcgtctgc
    25741 cactggacga taacggtatt ctgtttatcg aaaacgttgg caacctcgta tgcccggcca
    25801 gcttcgatct cggtgaaaaa cacaaagtgg cggtgctttc cgttaccgaa ggtgaagaca
    25861 aaccgctgaa atatccgcat atgtttgccg ccgcctcgct gatgctgctc aacaaagttg
    25921 acctgttgcc gtatctcaac tttgacgttg agaagtgcat cgcctgcgcc cgcgaagtca
    25981 atccagaaat tgaaatcatc cttatttccg ccaccagcgg cgaagggatg gaccagtggc
    26041 tgaactggct ggagacacag cgatgtgcat aggcgttccc ggccagatcc gcaccattga
    26101 cggcaaccag gcaaaagtcg acgtctgcgg cattcagcgc gatgtcgatt taacgttagt
    26161 cggcagctgc gatgaaaacg gtcagccgcg cgtgggccag tgggtactgg tacacgttgg
    26221 ctttgccatg agcgtaatta atgaagccga agcacgcgac actctcgacg ccttacaaaa
    26281 catgtttgac gttgagccgg atgtcggcgc gctgttgtat ggcgaggaaa aataatgcgt
    26341 tttgttgatg aatatcgcgc gccggaacag gtgatgcagt taattgagca tctgcgcgaa
    26401 cgtgcttcac atctctctta caccgccgaa cgccctctgc ggattatgga agtgtgcggt
    26461 ggtcataccc acgccatttt taaattcggc ctcgaccagt tactgcctga aaacgttgag
    26521 tttatccacg gtccgggttg cccggtgtgc gtactgccga tgggtagaat cgacacctgc
    26581 gtggagattg ccagccatcc ggaagtcatc ttctgtacct ttggcgacgc catgcgcgtg
    26641 ccggggaaac agggatcgct gttgcaggca aaagcacgcg gtgccgatgt gcgcatcgtt
    26701 tactcgccga tggatgcgtt gaaactggcg caggagaatc caacccgtaa agtcgtattc
    26761 ttcggcttag gttttgaaac caccatgccg accaccgcca tcaccctgca acaggcgaaa
    26821 gcccgcgatg tgcagaattt ttacttcttc tgccagcata ttacgcttat cccgacgctg
    26881 cgcagtttgc tggaacagcc ggataacggt atcgacgcgt tcctcgcgcc gggccacgtt
    26941 agtatggtta tcggcactga tgcctataat tttatcgcca gcgattttca tcgtccgctg
    27001 gtggtcgctg gtttcgaacc ccttgatcta ctgcaaggcg tggtcatgct ggtggagcag
    27061 aaaatagcgg cccacagcaa ggtagagaat cagtatcgtc gggtggtacc ggatgccggt
    27121 aacctgctgg cgcaacaggc aattgctgat gtgttctgtg tcaacggcga cagcgaatgg
    27181 cgcggcttag gcgtgattga atcttctggc gtgcacctga cgccggatta tcaacgattc
    27241 gatgccgaag cacatttccg cccagcaccg cagcaggtct gcgatgaccc gcgcgcgcgt
    27301 tgtggtgagg tattaacggg caaatgtaag ccgcatcaat gcccgctgtt tggtaacacc
    27361 tgtaatcctc aaaccgcatt tggtgcactg atggtctcct ccgaaggagc gtgcgccgcg
    27421 tggtatcagt atcgtcagca ggagaatgaa gcgtgaataa tatccaactc gcccacggca
    27481 gcggcggcca ggcgatgcag caattaatca acagcctgtt tatggaagcc tttgccaacc
    27541 cgtggctggc agagcaggaa gaccaggcac gtcttgatct ggcgcagctg gtagcagaag
    27601 gcgaccgtct ggcgttctcc accgacagtt acgtcattga cccgctgttc tttcctggcg
    27661 gtaatatcgg caagctggcg atttgcggca cagccaatga cgttgcggtc agtggcgcta
    27721 ttccgcgcta tctctcctgt ggctttatcc tcgaagaagg attgccgatg gagacactga
    27781 aagccgtagt gaccagcatg gcagaaaccg cgcgcgcggc aggcattgcc atcgttactg
    27841 gcgatactaa agtggtgcag cgcggcgcgg cagataaact gtttatcaac accgctggca
    27901 tgggcgcaat tccggcgaat attcactggg gcgcacagac gctaaccgca ggcgatgtat
    27961 tactggttag cggcacgctc ggggaccacg gggcgactat ccttaacctg cgtgagcagc
    28021 tggggctgga tggcgaactg gtcagcgact gcgcggtact gacgccgctt attcagacgc
    28081 tgcgtgacat tcccggcgtg aaagcgctgc gtgatgccac ccgtggtggt gtgaacgcgg
    28141 tagttcatga gttcgcggca gcctgcggtt gtggtattga aatttcagaa gtggcactgc
    28201 cggttaaacc tgccgtgcgc ggcgtttgcg aactgctggg actggacgcc cttaactttg
    28261 ccaacgaagg caaactggtg atcgccgttg aacgcaacgc ggcagagcaa gtgctggcag
    28321 cgttacattc ccatccactg gggaaagacg cggcgctaat tggtgaagtg gtggaacgta
    28381 aaggtgttcg tcttgccggt ctgtatggtg tgaaacgaac cctcgattta ccacacgccg
    28441 aacctcttcc gcgtatatgc taataaaatt ctaaatctcc tatagttagt caatgacctt
    28501 ttgcaccgct ttgcggtgct ttcctggaag aacaaaatgt catatacacc gatgagtgat
    28561 ctcggacagc aagggttgtt cgacatcact cggacactat tgcagcagcc cgatctggcc
    28621 tcgctgtgtg aggctctttc gcaactggta aagcgttctg cgctcgccga caacgcggct
    28681 attgtgttgt ggcaagcgca gactcaacgt gcgtcttatt acgcatcgcg tgaaaaagac
    28741 accccattaa atatgaagac gaaactgttc tggcacacgg tccggtacgc cgcattttgt
    28801 cgcgccctga tacgctgcat tgcagttacg aagaattttg tgaaacctgg ccgcagctgg
    28861 tcgcaggtgg gctatacccc aaatttggtc actattgcct gatgccactg gcggcggaag
    28921 ggcatatttt tggtggctgt gaatttattc gttatgacga tcgtccctgg agcgaaaaag
    28981 agttcaatcg tctgcaaaca tttacgcaga tcgtttctgt cgtcaccgaa caaatccaga
    29041 gccgcgtcgt caacaatgtc gactatgagt tgttatgccg ggagcgcgat aacttccgca
    29101 tcctggttgc cattaccaac gcggtgcttt cccgcctgga tatggacgaa ctggtcagcg
    29161 aagtcgccaa agaaatccat tactatttcg acattgacga tatcagtatc gtcttacgca
    29221 gccaccgtaa aaacaaactc aacatctact ccactcacta tcttgataaa caacatcccg
    29281 cccacgaaca gagcgaagtc gatgaagccg gaaccctcac cgaacgcgtg ttcaaaagta
    29341 aagagatgct gctgatcaat ctccacgagc gggacgattt agccccctat gaacgcatgt
    29401 tgttcgacac ctagggcaac cagattcaaa ccttgtgcct gttaccgctg atgtctggcg
    29461 acaccatgct gggcgtgctg aaactggcgc aatgcgaaga gaaagtgttt accactacca
    29521 atctgaattt gctgcgccag attgccgaac gtgtggcaat cgctgtcgat aacgccctcg
    29581 cctatcagga aatccatcgt ctgaaagaac ggctggttga tgaaaacctc gccctgaccg
    29641 agcagctcaa caatgttgat agtgaatttg gcgagattat tggccgcagc gaagccatgt
    29701 acagcgtgct taaacaagtt gaaatggtgg cgcaaagtga cagtaccgtg ctgatcctcg
    29761 gtgaaactgg cacgggtaaa gagctgattg cccgcgcgat ccataatctc agtgggcgta
    29821 ataatcgccg catggtcaaa atgaactgcg cggcgatgcc tgccggattg ctggaaagcg
    29881 atctgtttgg tcatgagcgt ggggctttta ccggtgccag cgcccagcgc attggtcgtt
    29941 ttgaactggc ggataaaagc tctctgttcc tcgacgaagt gggcgatatg ccactggagt
    30001 tacagccgaa gttgctgcgt gtattgcagg aacaggagtt tgaacgtctc ggcagcaaca
    30061 aaatcattca gacggacgta cgtttaattg ccgcgactaa ccgcgatctg aaaaaaatgg
    30121 tcgccgaccg agagttccat agcgatctct attaccgcct gaacgtattc ccgattcacc
    30181 tgccgccact acgcgagcgt ccggaagata ttccgctgct ggcgaaagcc tttaccttca
    30241 aaattgcccg tcgtctgggg cgcaatatcg acagcattcc tgccgagacg ctgcgcacct
    30301 tgagcaacat ggagtggccg ggtaacgtac gcgaactgga aaacgtcatt gagcgcgcgg
    30361 tattgctaac acgcggtaac gtgctgcagc tgtcattgcc agatattgtt ttaccggaac
    30421 ctgaaacgcc gcctgccgca acggttgtcg cccaggaggg cgaagatgaa tatcagttga
    30481 ttgtgcgcgt gctgaaagaa accaacggcg tggttgccgg gcctaaaggc gctgcgcaac
    30541 gtctggggct gaaacgcacg accctgctgt cacggatgaa gcggctggga attgataaat
    30601 cggcattgat ttaactgcaa attgccggac agatctgcct gtccggcata ctattcacga
    30661 ggttttttcg gacgatattt ttccggcagt tctggtaccg gacgcttgtc atcgatgaga
    30721 tgacgcacgg ttaagatcgg atgacgccac agcattctcg gcccggccca acgcataatc
    30781 tgtttcatct cttcacgctt tgcaggctgg taacagtgca ccggacactg cttacaggct
    30841 ggtttctctt cgccgaacac acatttatcc agccgctttt gcgcgtaaac aaacaacgcc
    30901 tcgtaatgct ccggctccgc tgacgcctgc gggcatttcg cttgataaag atcgatcatt
    30961 tttttaatcg tcagtttttc acgagagata cgcttgccgg acatgctgcc tccatactct
    31021 taaggtgtat ttatattaca acttaatttg aaaggggact atggctctga aggaggaggt
    31081 ttattcaacc gacgcaattt tgcgtacttg cttcataagc atcacgcaaa agctgcaaaa
    31141 cagcatcttt cccggaacca gcatcaagaa ctcgccgttc gcttcttccc ctgaaatgat
    31201 taactccggt atcatgtgcg ccttatgtga ttacaacgaa aataaaaacc atcacacccc
    31261 atttaatatc agggaaccgg acataacccc atgagtgcaa tagaaaattt cgacgcccat
    31321 acgcccatga tgcagcagta tctcaggctg aaagcccagc atcccgagat cctgctgttt
    31381 taccggatgg gtgattttta tgaactgttt tatgacgacg caaaacgcgc gtcgcaactg
    31441 ctggatattt cactgaccaa acgcggtgct tcggcgggag agccgatccc gatggcgggg
    31501 attccctacc atgcggtgga aaactatctc gccaaactgg tgaatcaggg agagtccgtt
    31561 gccatctgcg aacaaattgg cgatccggcg accagcaaag gtccggttga gcgcaaagtt
    31621 gtgcgtatcg ttacgccagg caccatcagc gatgaagccc tgttacagga gcgtcaggac
    31681 aacctgctgg cggctatctg gcaggacagc aaaggtttcg gctacgcgac gctggatatc
    31741 agctccgggc gttttcgcct gagcgaaccg gccgaccgcg aaacgatggc ggcagagctg
    31801 caacgcacta atccggcgga actgctgtat gcagaagatt tcgccgagat gtcgctgatt
    31861 gaaggccgtc gcggcctgcg ccgtcgcccg ctgtgggagt ttgaaatcga caccgcgcgc
    31921 cagcagttga atctgcaatt tggcacccgc gatctggtcg gttttggcgt cgagaacgcg
    31981 ccgcgcggac tttgtgctgc cggttgtctg ttgcagtatg cgaaagatac ccaacgcacg
    32041 accctgccgc atattcgttc tatcaccatg gaacgtgagc aggacagcat cattatggat
    32101 gccgcgacac gtcgtaacct ggaaattact cagaacctgg cgggcggtgc ggaaaatacg
    32161 ctggcttctg tgctcgactg caccgtcacg ccgatgggta gtcgtatgct gaaacgctgg
    32221 ctgcatatgc cagtgcgcga tacccgcgtg ttgcttgagc gccagcaaac tattggcgca
    32281 ttgcaggatt tcaccgccga gttgcagccg gtactgcgtc aggtcggcga cctggaacgt
    32341 attctggcac gtctggcttt acgaactgct cgcccacgcg atctggcccg tatgcgccac
    32401 gctttccagc aactgccgga gctgcgtgcg cagttagaaa ctgtcgatag tgcaccggta
    32461 caggcgctac gtgagaagat gggcgagttt gccgagctgc gcgatctgct ggagcgagca
    32521 atcatcgaca caccgccggt gctggtacgc gacggtggtg ttatcgcatc gggctataac
    32581 gaagagctgg atgagtggcg cgcgctggct gacggcgcga ccgattatct ggagcgtctg
    32641 gaagtccgcg agcgtgaacg taccggcctg gacacgctga aagttggctt taatgcggtg
    32701 cacggctact acattcaaat cagccgtggg caaagccatc tggcacccat caactacatg
    32761 cgtcgccaga cgctgaaaaa cgccgagcgc tacatcattc cagagctaaa agagtacgaa
    32821 gataaagttc tcacctcaaa aggcaaagca ctggcactgg aaaaacagct ttatgaagag
    32881 ttgttcgacc tgctgttgcc gcatctggaa gcgttgcaac agagcgcgag cgcgctggcg
    32941 gaactcgacg tgctggtgaa cctggcggaa cgggcctata ccctgaacta cacctgcccg
    33001 accttcattg ataaaccggg cattcgcatt accgaaggtc gccatccggt agttgaacaa
    33061 gtactgaatg agccatttat cgctaacccg ctgaatctgt cgccgcagcg ccgtatgttg
    33121 atcatcaccg gtccgaacat gggcggtaaa agtacctata tgcgccagac cgcactgatt
    33181 gcgctgatgg cctacatcgg cagctatgta ccggcacaaa aagtcgagat tggacctatc
    33241 gatcgcatct ttacccgcgt aggcgcggca gatgacctgg cttccgggcg ctcaaccttt
    33301 atggtggaga tgactgaaac cgccaatatt ttacataacg ccaccgaata cagtctggtg
    33361 ttaatggatg agatcgggcg tggaacgtcc acctacgatg gtctgtcgct ggcgtgggcg
    33421 tgcgcagaaa atctggcgaa taagattaag gcattgacgc tgtttgccac ccactatttc
    33481 gagctgaccc agctaccgga gaaaatggaa ggcgtcgcca acgtacatct cgatgcgctg
    33541 gagcacggcg acactatcgc ctttatgcac agcgtgcagg acggcgcggc gagcaaaagc
    33601 tacggtctgg cggttgctgc tctggcgggc gtaccgaaag aggttattaa gcgcgcacgg
    33661 caaaaactgc gtgagctgga aagcatttcg ccgaacgccg ccgcgacgca agtggatggt
    33721 acgcagatgt ctttgctgtc agtgccagaa gaaacttcgc ctgcggtcga agcactggaa
    33781 aatctcgacc cggattcact gactccgcgt caggcgctgg agtggattta tcgcttgaag
    33841 agtctggtgt aataacaatt cccgatagtc ttttgctatc gggaatatta acgacaactg
    33901 acgaatcaaa taaaaatacc ctgtataata ggaaagctta ttttacaggg taaaaccatg
    33961 ccatctacac gctatcaaaa aatcaatgcc catcactatc gccatatatg ggtcgttggt
    34021 gatattcatg gtgaatatca gttattacaa tcccgttaac atcaactctc tttttaccct
    34081 gaaaccgact tacttatttc cacaggcgat aatattgatc gtgggccgaa gagtcttaac
    34141 gtgctgcgcc tgctaaacca accctggttt acgtcggtta aaggcaacca cgaagcgatg
    34201 gcgttggatg cattcgaaac tggcgatgga aatatgtggc ttgccagcgg tggtgactgg
    34261 tttttcgatt taaatgattc agagcaacaa gaggcaatag atctgttgct gaaattccat
    34321 caccttccac atattattga aatcattaat gacaacataa aatatgccat c
//
LOCUS       DAZPLI010000029        32837 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZPLI010000029 DAZPLI010000000
VERSION     DAZPLI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.029"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            267..1925
                     /locus_tag="ACHUU3_001352"
     CDS             267..1925
                     /locus_tag="ACHUU3_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR2081470.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="ACHUU3_001353"
     CDS             complement(1922..2878)
                     /locus_tag="ACHUU3_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR2081471.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="ACHUU3_001354"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="ACHUU3_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR2081472.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="ACHUU3_001355"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="ACHUU3_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR2081473.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHUU3_001356"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHUU3_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR2081474.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6534..6608
                     /gene="yljB"
                     /locus_tag="ACHUU3_001357"
     CDS             6534..6608
                     /gene="yljB"
                     /locus_tag="ACHUU3_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR2081475.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6707..7027
                     /gene="clpS"
                     /locus_tag="ACHUU3_001358"
     CDS             6707..7027
                     /gene="clpS"
                     /locus_tag="ACHUU3_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR2081476.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7058..9334
                     /gene="clpA"
                     /locus_tag="ACHUU3_001359"
     CDS             7058..9334
                     /gene="clpA"
                     /locus_tag="ACHUU3_001359"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR2081477.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9740..9827)
                     /locus_tag="ACHUU3_001360"
     tRNA            complement(9740..9827)
                     /locus_tag="ACHUU3_001360"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9791..9793),aa:Ser,seq:gga)
     gene            complement(10081..10299)
                     /gene="infA"
                     /locus_tag="ACHUU3_001361"
     CDS             complement(10081..10299)
                     /gene="infA"
                     /locus_tag="ACHUU3_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR2081478.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10584..11288)
                     /gene="aat"
                     /locus_tag="ACHUU3_001362"
     CDS             complement(10584..11288)
                     /gene="aat"
                     /locus_tag="ACHUU3_001362"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR2081479.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="ACHUU3_001363"
     CDS             complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="ACHUU3_001363"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR2081480.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="ACHUU3_001364"
     CDS             complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="ACHUU3_001364"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR2081481.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="ACHUU3_001365"
     CDS             complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="ACHUU3_001365"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR2081482.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16450..16944
                     /gene="lrp"
                     /locus_tag="ACHUU3_001366"
     CDS             16450..16944
                     /gene="lrp"
                     /locus_tag="ACHUU3_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR2081483.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17079..21146
                     /gene="ftsK"
                     /locus_tag="ACHUU3_001367"
     CDS             17079..21146
                     /gene="ftsK"
                     /locus_tag="ACHUU3_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR2081484.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21301..21912
                     /gene="lolA"
                     /locus_tag="ACHUU3_001368"
     CDS             21301..21912
                     /gene="lolA"
                     /locus_tag="ACHUU3_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR2081485.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21923..23266
                     /gene="rarA"
                     /locus_tag="ACHUU3_001369"
                     /gene_synonym="mgsA"
     CDS             21923..23266
                     /gene="rarA"
                     /locus_tag="ACHUU3_001369"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR2081486.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23357..24649
                     /gene="serS"
                     /locus_tag="ACHUU3_001370"
     CDS             23357..24649
                     /gene="serS"
                     /locus_tag="ACHUU3_001370"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR2081487.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24888..27332
                     /gene="dmsA"
                     /locus_tag="ACHUU3_001371"
     CDS             24888..27332
                     /gene="dmsA"
                     /locus_tag="ACHUU3_001371"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFR2081488.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27343..27960
                     /gene="dmsB"
                     /locus_tag="ACHUU3_001372"
     CDS             27343..27960
                     /gene="dmsB"
                     /locus_tag="ACHUU3_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR2081489.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27962..28825
                     /gene="dmsC"
                     /locus_tag="ACHUU3_001373"
     CDS             27962..28825
                     /gene="dmsC"
                     /locus_tag="ACHUU3_001373"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFR2081490.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28861..29487)
                     /gene="ycaC"
                     /locus_tag="ACHUU3_001374"
     CDS             complement(28861..29487)
                     /gene="ycaC"
                     /locus_tag="ACHUU3_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR2081491.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29802..30950
                     /locus_tag="ACHUU3_001375"
     CDS             29802..30950
                     /locus_tag="ACHUU3_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2081492.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            31160..32590
                     /locus_tag="ACHUU3_001376"
     CDS             31160..32590
                     /locus_tag="ACHUU3_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR2081493.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(32591..>32837)
                     /locus_tag="ACHUU3_001377"
     CDS             complement(32591..>32837)
                     /locus_tag="ACHUU3_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR2081494.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         8057 a         8032 c         8814 g         7934 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
     6661 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
     6721 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
     6781 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
     6841 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
     6901 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
     6961 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
     7021 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
     7081 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
     7141 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
     7201 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
     7261 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
     7321 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
     7381 tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
     7441 agtcagccgt ctcgatgtgg tgaattttat ctctcatggc acgcgtaaag acgagccgac
     7501 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
     7561 tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa tcgacccact
     7621 gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
     7681 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg aaggtcttgc
     7741 ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga tttactctct
     7801 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
     7861 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
     7921 caccattatc ggtgcggggg cagcgtctgg tggtcaggtt gatgcggcta acctaatcaa
     7981 accgttgctc tccagcggta aaattcgtgt aattggttcg acaacctatc aggagttcag
     8041 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
     8101 accgtcaatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
     8161 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
     8221 catcaatgat cgtcatctgc cggataaagc gattgatgtt atcgacgaag cgggcgctcg
     8281 cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
     8341 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
     8401 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata aagccattga
     8461 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
     8521 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
     8581 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
     8641 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
     8701 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
     8761 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
     8821 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ccaacgccgg
     8881 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
     8941 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
     9001 ctggtttgat catctgtcaa ccgacgtgat ccatcaggtg gtggataaat tcatcgtcga
     9061 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
     9121 ctggctggcc gagaaaggtt acgaccgggc aatgggcgct cgtccgatgg cgcgtgtcat
     9181 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
     9241 cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
     9301 tgcacaaaag cacaaggcgg aagcagcgca ttaatctaat tgttaggtag gttggtgaag
     9361 tccgtaatct cgaaagaggt tacggacttt ttgtttatgg ggtggaacgg tgaaaaccct
     9421 atttttggag gtgaaggtaa tttgttgata attagtgctg ctggaaaagg taaggataaa
     9481 aaagggtgac agcaggaaaa tgagatggtt ttgctttatt aacaacggac taaacgtgta
     9541 gtatttgagt tcactgccgt acaggcagct tagaaatcga cggggtgcgg taaaaccttt
     9601 gcgaacgcgt tcactgccgt acaggcagct tagaaattca caggtaacat actccacccg
     9661 cccaccatgt tcactgccgt acagaaagat aaaatgcgaa aaaaaagctc gcactttcgt
     9721 acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt
     9781 atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgttttgct
     9841 accaaacctc atgggtggca acggggcgct actataggga gttggagtaa aacggtcaag
     9901 aagaatttta atgataatta ttgtttgctc atactgtaaa caagttgtgc agtatatcta
     9961 catcgagaca agttacggac ttatacttcc aaagtacttc atacatatca caaaataaaa
    10021 aggccggtta aaccgacctt ttactcgttc tttctcttcg cccatcaggc ggtaaaacaa
    10081 tcagcgacta cggaagacaa tgcggccttt gctcaggtcg tacggggtca gttcaacagt
    10141 cactttgtcg cccgtcagga tgcggatgta gtttttgcgc attttaccgg agatgtgtgc
    10201 agtaaccacg tgaccgtttt ctaactctac gcggaacatg gtattaggca acgtttcaag
    10261 aacggtacct tgcatttcaa tattgtcttc tttggccatc taatcctctg gggtatcact
    10321 accgtaattt gaaccggcaa gataatgccg aagttctgta aataagtaaa gatttgcgcg
    10381 ctaaatcgca acaaacaggt tcggcacatt actccgaaaa cacacggcta agccgcacca
    10441 aaagcgcaac gtataaggga gcggtgagat aaacgatggg cgttacctga cgcgaaaaat
    10501 tccttatcgg cagcggggta atgagcataa ccaactctgc gaccgcaatt ataacactct
    10561 ggggagaaat gtgccgaaaa cattcatttt tgtggtgaaa acaagcatcg tggcacccag
    10621 aaattattcg gcaatcgtcc gaggcgcatt tgattgagat aattcagata atcccgacgg
    10681 ggaatttcgc aggcaccaag cgatgctgtg tgatcgttaa ggacctggca gtcgataagc
    10741 ttaccgccat gactgataaa ttcctcacag aataccagaa gcgccgtttt agacgcattt
    10801 tccatccggc tgaacatgga ctcgccacaa aatagcgttc cctgggccac gccgtacata
    10861 ccgccgacaa gctcatcttc atgccagact tcaatggagt gggcatgacc gagttcgtga
    10921 aggcgatggt aggcttcgac cacgccacgc gtgatccagg ttccttcttc gcgatcgctg
    10981 gcacaacctt caatgacctg accaaaagcg taattcatcg tgacacgata gggcgagcgt
    11041 ttatgaaatc gcttcatact acggctgata tgcagtgatt caggccaaag caccgcgcga
    11101 ggatcgggcg accaccagag gatgggggcg cctggagaaa accacggaaa aataccacgc
    11161 tggtaagcca ttaacaggcg cgcagggcta agatcgcccc ccagagccag caggccgtta
    11221 ggctcacgta atgcgccttc cggggaaggg aaggctattg aatggcgaga aagctgaacc
    11281 aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc aataatagct tacaaaccct
    11341 gcttaaactg gtaataacgc ccctgtctgg caagcagttc tgcgtgagta ccttgctcaa
    11401 taatttgccc gttgtccatc actattattt gttggaaacg agagagtccg cgaagtcgat
    11461 gggtgaccat taacaccgtt ttctcacgca tcatttctgc aagcaattca aggatctggc
    11521 tttcggttgt ggcatctaag ccttcggtag gttcatccag caacaccagt ggcgcatcat
    11581 gtaacagcgc acgggcgata gccagacggc gcagttcacc accggagagc tggcgtccgc
    11641 cttcacctaa ccaactgttg agacctgcgt cctcgagcag cttctccaga ccaacgcgac
    11701 gcaagatctc cgacagagcc tcatcactac tgccaggcga ggcgagtaac agattatcac
    11761 gcagcgtggc gctaaacaga tgcactcgct gaggaacaac gctgatggtc tgtcgtagag
    11821 ccgcttcatt caggctggct atggggctat cgttaagcaa aatctcgccc tgttgcgggt
    11881 cccaagcgcg ggtcagcagt tgtaacagtg ttgatttgcc gcatccggtt cgcccgagaa
    11941 tcgcaatatg ttccccggcg tttacctgaa gagaaatccc tttaagtgcc tgttgggatt
    12001 gctccggata agtgaactga acatcccgta atgtcagcga aacgcgatcg gcaacacgag
    12061 tttgggtatc agggaaggtg acttccggtt tttgatccgt taaatcagtg atccgaacgg
    12121 cagaggcaat gacttgcccc agatgctgaa atgcacccgt tactggtgcc agtgcttcaa
    12181 acgcggctaa cgcgcagaag acaaacaggg caattaacgc gccgggttga gcattgccgc
    12241 caacgccgcc agacgccatc cacagcatca ggatcaccgc taacgcgcca atgagcagca
    12301 ttatcgcttg cgacaatgcg gtcagttcag attgacggcg ttgcgcttcc agccattgaa
    12361 tttctgtatt ctctagttgc gtgcgataac gatcgctggc accaaaaatg gtcagctcag
    12421 cttgcccttg cagccaggcc gtcagttgtt ggcgatactg tccgcgaaga tgagtcagat
    12481 tttgcccggt gctttttccc gcacgataaa acagcggtgg catcaggaaa agcgtcagta
    12541 acataatgcc gcccagcgta aaggcgaggg tgaaatcaag gaaacttaac ccgactgtca
    12601 ccaccataat caccacaaaa gcgcccacca gcggcgagat aacgcgcagg taaagatgat
    12661 cgagcgtatc aacatccgcc accacgcgat tgagcaattc gccctgacga tagcgcgcca
    12721 gtccggcagg ggagaggggc aacaatttgc tgaaggtgta aatgcgcaga tgctgcaaca
    12781 cgcggaaagt cgcgtcgtga cttaccagac gttcaaaata acgcccggca gtacgggtga
    12841 ttgcggcacc acgcacgccc gcagcgggta gcatatagtt gaagctgtac agtccggcaa
    12901 cccccgcaac cgctgaggcc gagaggaacc agccggaaag tgtcaacaga ccgatactgg
    12961 cgagcagcgt cacaattgcc agcacaatac caagacttaa catccattta tgacgtttat
    13021 acagtgccag atagggtagc aaagcgcgca tttaaatctc ctcctgacga tgggccagta
    13081 atgtggcgaa tgggccacca gccacactta attccgcgta acgtccttgc tcaataatct
    13141 gaccatcctg cattacccaa atgacatccc agtcagcaag atcttctaac tggtgggtga
    13201 ccattaacgt tgtctggcgc agagaggcgg cattcagcgc ctccattacg cgctgttcac
    13261 tgtgagcatc aaggctggca gcgggttcat ccaacagtaa tagcgaacag ggatttagta
    13321 acgcacgggc caccgccacg cgctgcgcct gccccacgga aaggcgggca gcctggtcgc
    13381 caacaggcgt atcaacgcct tgtggcagga gcggtagaaa ctcgctgacc caggcgttat
    13441 ccagcgctgc ttgtaattca tgttcgctgg catcaggtcg cgccagtagt acgttatccc
    13501 gtaatgttgc tgccggtaat tgtggatttt gcccaaccca ggagagatgt ttacgccatg
    13561 attctggtga taaatcgcgt aattctatcc cgttgattcg tagcgatccc tgatatgaga
    13621 gaaaaccaga aagcgcgttc aacagtgaac ttttacctga accgctgcga ccaaccaaca
    13681 cggcacgttg gcctgctggc aaagtaaagt tcagcggtcc ggccagcgtt ttaccttccg
    13741 gcgacgtgat aaacagatcc tcggcctcaa tggtcaccgg atcggtcgat gctaattcca
    13801 cctcgccacg ctgcggatgg gcgagcgggg tttccataaa cgttttcaga ctgtcagctg
    13861 caccaacagc ctgggcttta gcatgataaa acgtaccgag atcgcgtaat ggctggaaaa
    13921 actctggcgc aaggatcagg gccagaaaac ccgcagccag cgtcacgcca gtatcgtagt
    13981 gaccaaaatc cagctcgccg agataggaaa aaccaaagta gaccgccacc agagcaattg
    14041 acagcgaggt aaaaaattcg agaatgccgg aggataaaaa tgccagccgt agcacttcca
    14101 ttgtccgttg gcggaaatct tccgaagcag aacgaatact ttcaatttca gcttcaccac
    14161 gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc gaggaaatgc ccacttaagc
    14221 gagcaagagc gagaaagtta cgtcggttag catcggcagc ccccattcca accagcgcca
    14281 taaacaacgg aattaacggt gcagtgccca gcagaatgag cgccgcagcc cagtttgagg
    14341 ggaagattgc caccacaatc agcaacggca ccgacactgc cagcgccatt tgcggcaggt
    14401 agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag taccagcgtc gcccagctcc
    14461 ccgcaggttt accctgaatc cacgctggcc ctgcttgttg cagacggtcg agaacctgac
    14521 ggcggatggc aaagcggata tgctgcccgg cgtgataacc cacccgttcg cgtaaccaga
    14581 ccgcccatgc gcgcagtaca aaggtcagaa ccagtaacgt aaagggaagc agcagggctt
    14641 cacggggaat attctccata atcatatgtt gcagaattcg cgccatgaac caggcctggg
    14701 caatgatcaa tatgccgctt acaaagccca gcagacgaga aatattcagc caacgttggg
    14761 agatgacgct ttgctgtttt aaccagcggg ttaactcttt ttgacgagat ttattcattg
    14821 cacgcttagc aggtgagtta tcagaattat ttgcagagca atgttacaac ggggaaaaaa
    14881 taaaggcgac ccatagtcgc aaggtgtcgc cttctttact tttgttactg atttgtaaaa
    14941 ttattttgcg tcagctaaac catcgaggta gcgttccgca tcaagtgctg ccatgcagcc
    15001 agtaccggcc gaagtaatgg cctggcgata aatgtgatcc atcacgtcgc ctgcggcaaa
    15061 gacgccagga atgctggtct gggtggcatt accatgaata cccgactgta ctttgatgta
    15121 gccgttttcc agttccagct gcccttcgaa aatcgcagta ttcgggctgt gaccgatagc
    15181 aacaaacaga ccggcaacgt cgagtgactc gatgttatcg ctgttttgcg tatcgcgcag
    15241 acgaacgcca gtgacaccca tttgatcgcc ggtcacttct tccagcgtac ggttggtgtg
    15301 cagaatgatg ttgccgttct ccactttatc catcagacgc ttaataagga ttttttccgc
    15361 gcggaaaccg tcacggcggt gaatcagatg cacttccgaa gcgatgttag acagatacag
    15421 cgcctcttca accgcggtat tgccgccgcc gatgaccgca actttctggt tgcgatagaa
    15481 gaaaccgtcg caggttgcac aagcagaaac cccacggcct ttaaaggctt cttcagaggg
    15541 caggccgaga tagcgtgcag aagctccggt ggcaataatc agcgcgtcgc aagtgtattc
    15601 gccgttatcg ccattcagac ggaacggacg gttttgcaga tccaccttgt tgatatgatc
    15661 aaaaatgatc tcagtttcaa acttggtggc atgttcgtgc atgcgctcca ttaataacgg
    15721 accggtcaga tcgtttggat caccaggcca gttttccact tccgtggtgg tggtcagttg
    15781 gccgcctttt tccatgccgg taatcagcac aggttgcagg ttggcgcgcg ccgcgtagac
    15841 agcagcggtg tatcccgccg ggcctgaacc caggataagc agtttactgt gtttggtcgt
    15901 gcccatgaga tccccatagt tgttggcaga caatgggcag gattgtaggg aatttacaga
    15961 cgtaaaaaaa gagtatgacg attttgttaa caatttgtgc aatcggcagc atcgataagc
    16021 aggtcaaatt ctcccgtcat tatcacctct gctacttaaa tttcccgctt tataagccga
    16081 ttaaatgatg aataaacgcc cctgttaatg aatatctggc atgttgtact aaaaatcgat
    16141 gttttgcttt gacaatcccc tggtgttttg cgaaaacatt cgaggaagaa aaaaacagta
    16201 ttcttatatg cgcataacca tgcatgtaaa taccatgttt accgtgctag tgaaatctac
    16261 gtatggcgtg gacagacgcc attcgtgatg tcgatagctg ccacaaggca acggtcttct
    16321 caccgtagac ccaggcattg cgcgccgtga atcttcatga tttcggtcta tcgtgacggg
    16381 tagcgactct gaacagtgat gtttcagggg cagacaggag tagggaagga atacagagag
    16441 acaataataa tggtagatag caagaagcgc cttggcaaag atctcgaccg tatcgatcgt
    16501 aacattctta atgagttgca aaaggatggg cgtatttcta acgtcgagct ttctaaacgt
    16561 gtgggacttt ccccaacgcc gtgccttgag cgtgtgcgtc ggctggaaag acaagggttt
    16621 attcagggct atacggcgct gcttaacccc cattatctgg atgcatcact tctggtattc
    16681 gttgagatta ctctgaatcg tggcgcaccg gatgtgtttg aacaattcaa taccgctgta
    16741 caaaaacttg aagaaattca ggagtgtcat ttagtatctg gtgatttcga ctacctgttg
    16801 aaaacacgcg tgccggatat gtcagcttac cgtaagttgc tgggggaaac cctgctgcgt
    16861 ctgcctggcg tcaatgacac acggacatac gttgttatgg aagaagtcaa gcagagtaat
    16921 cgtctggtta ttaagacgcg ctaacacgga acaggtgcaa aatcggcgta ttttgattac
    16981 actcctgtta atccatacag caacagtact ggggtaacct ggtactgttg tccgttttag
    17041 catcgggcag gaaaagcctg taacctggag agcctttctt gagccaggaa tacactgaag
    17101 acaaagaagt cacattgaca aagttaagta gcggccgccg ccttctggag gcgttgctga
    17161 tccttattgt cctgtttgcc gtctggttga tggctgcctt actaagcttt aacccttcgg
    17221 accccagctg gtcgcaaacg gcctggcatg aacctatcca taatttaggt gggatgcccg
    17281 gtgcgtggtt ggcagatacg ctgttcttta tttttggcgt gatggcttac accattcccg
    17341 tcattattgt cggcggttgt tggtttgcct ggcgtcatca gtccagcgac gaatacattg
    17401 attattttgc cgtttcgcta cgcatcattg gcgttttggc gctcatcctt acctcctgtg
    17461 gtctggcggc aatcaacgct gacgatatct ggtattttgc ctccggtggc gtcattggca
    17521 gcttactaag cactacgcta caaccactgc tacacagtag cgggggaact attgcgctgc
    17581 tctgcgtttg ggcagcgggc ctgacgttgt tcaccggttg gtcatgggtg accattgctg
    17641 aaaaactcgg cggctggatt ttaaacattc tcaccttcgc cagtaatcgt acccgtcgcg
    17701 atgatacctg ggtcgatgaa gatgagtatg aagacgacga agagtatgaa gatgaaaatc
    17761 acggcaaaca gcatgaatca cgccgtgccc gtattcttcg cggcgcgcta gcgcgtcgta
    17821 aacggttggc ggaaaaattc attaatccga tggggcggca aacagacgct gcgttgttct
    17881 ccggtaagcg gatggatgat gacgaagaga ttacctacac tgcacgcggt gtggctgccg
    17941 accctgacga cgtcctattt tcgggcaatc gtgcaacgca gccagaatat gacgaatacg
    18001 atccattatt aaacggtgcg ccaattaccg aacctgtcgc tgtagcagct gctgctacca
    18061 cggcggcaca aagctgggct gcgccggttg aacctgtgac tcagacgccg cctgttgcct
    18121 ctgttgatgt tccaccttcg caacctacag tagcctggca gcctgtaccg ggtccacaaa
    18181 cgggagagcc ggttattgct cctgcaccgg aaggttaccc acagcagcca caatatgcgc
    18241 agcctgcagt gcaatataat gagccgctgc aacaaccagt acagccacag cagccgtatt
    18301 atgcacctgc agctgaacaa cctgtgcaac aaccttacta cgctcctgcg gcggaacaac
    18361 ctgttcagca accgtattac gcccctgcgc cagaacaacc ggtggcaggt aacgcctggc
    18421 aagccgaaga gcagcaatcc acttttgctc cacagtctac ataccagact gagcaaactt
    18481 atcagcaacc agccgctcag gagccgttgt accaacagcc gcaacccgtt gaacagcagc
    18541 ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc cgcgcgtccg ccactttact
    18601 attttgaaga agtggaagag aagcgagccc gtgaacgtga acaacttgcg gcctggtatc
    18661 aaccgattcc agaaccggtt aaagaaccag aaccgatcaa atcttcgctg aaagcacctt
    18721 ctgttgcagc agtacctcca gtagaaaccg ctgccgctgt ttctccgctg gcatctggcg
    18781 tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt tgccgcgcca gtcttcagtc
    18841 tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg gattggcccg cagttgccac
    18901 gaccgaaacg tatccgcgtg ccaactcgcc gtgaattggc gtcttacggt attaagctgc
    18961 cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca gcgcaatcag tacgattctg
    19021 gcgatcagta caacgatgat gaaatcgatg cgatgcagca ggatgaactg gcacgtcagt
    19081 tcgcccagac acagcagcaa cgctatggcg aacagtatca acatgatgtg cccgtaaacg
    19141 cagaagatgc agatgctgcg gcagaggctg aactggctcg tcagtttgcg caaactcaac
    19201 aacaacgtta ttccggcgaa caaccggctg gggcgaatcc gttctcgctg gatgattttg
    19261 aattttcgcc aatgaaagcg ttgctggatg atggtccaca cgaaccgttg tttacgccaa
    19321 ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc gcagcagcag tatcagcagc
    19381 cacaacagcc ggttgcgcca cagcagcaat atcagcagcc gcaacagccg gttgcgccgc
    19441 agccacaata tcagcagccg caacaaccgg ttgcgccaca gcagcaatat cagcagccgc
    19501 aacaaccagt tgcgccgcag ccgcagtatc aacagccgca acaaccggtt gcgccgcagc
    19561 agcagtatca gcagccacaa cagccagttg cgccacaacc gcaggatacc ctgcttcatc
    19621 cgctgttgat gcgtaatggc gacagccgtc cgttgcataa accgacgacg ccgctgcctt
    19681 ctctggattt gctgacaccg ccgccgagcg aagtggagcc ggtagatacc tttgcgcttg
    19741 aacaaatggc tcgcctggtg gaagcgcgtc tggctgattt ccgtattaaa gccgatgttg
    19801 tcaattactc tccggggccg gttatcactc gctttgaatt gaacctggca ccgggcgtaa
    19861 aagcggcgcg catttctaac ttgtcacggg accttgcccg ttcactttcg acggtggcgg
    19921 tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg tctggagtta ccgaataaaa
    19981 aacgacaaac cgtttatctg cgcgaagttt tggataacgc caaattccgc gataatccgt
    20041 cgccattaac cgtggtgctg ggtaaagata tcgccggtga gccggtggtt gccgatctgg
    20101 cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc cggtaaatct gtcggtgtga
    20161 acgcgatgat cctgagcatg ctttataaag cacagccaga agatgtgcgt ttcatcatga
    20221 tcgacccgaa aatgctggag ctttcggttt atgaaggcat tccgcatctg ttaacggaag
    20281 tcgttactga tatgaaagat gccgccaacg cgctgcgctg gtgtgttaac gagatggagc
    20341 gtcgttataa actgatgtct gcgctgggtg tgcgtaatct ggcgggttat aacgaaaaaa
    20401 ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc gtactggaag ccgggtgaca
    20461 gtatggatgc ccagcatccg gtgctgaaaa aagaaccata cattgtggtg ttggttgacg
    20521 aatttgccga cctgatgatg acggtaggta aaaaagtgga agagctgata gcacgtctgg
    20581 cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc aactcagcgt ccatcggttg
    20641 atgttattac tggtctgatt aaagcgaata ttccgacccg tatcgccttt accgtatcca
    20701 gtaagattga ctcacgtacc attcttgatc aggctggcgc ggaatcactg ctggggatgg
    20761 gggatatgct ctactctggg ccgaactcca cgttgccggt acgtgtccat ggtgcttttg
    20821 ttcgcgatca ggaagttcat gccgtggtgc aggactggaa agcgcgtggt cgcccacagt
    20881 atgttgatgg catcacttcc gacagtgaaa gcgaaggtgg cgcgggcggt ttcgatggcg
    20941 ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt tgtcactgaa aaacgcaaag
    21001 cgtcaatttc tggcgtacag cgtcagttcc gcattggtta taaccgtgca gcgcgtatta
    21061 tcgaacagat ggaagcgcag gggattgtca gcgaacaggg gcacaacggt aatcgtgaag
    21121 tgctggctcc accgccgttt gactaattaa tgcattgccg gataaggcgc ggtagcgtcg
    21181 catccggcac tctatcaact gaaaattcag tattttcttc tttcctcaag ctgattatta
    21241 gcctggaata gagagtagag ggaactcccg atcgggagtg acgtaatttg aggaataatg
    21301 atgaaaaaaa ttgccatcac ctgtgcatta ctctcaagct tagtagcaag cagcgtttgg
    21361 gctgatgccg caagcgatct gaaaagccgc ctggataaag tcagcagctt ccacgccagc
    21421 ttcacacaaa aagtgactga cggtagcggc gcggcggtgc aggaaggtca gggcgatctg
    21481 tgggtgaaac gtccaaactt attcaactgg catatgacac aacctgatga aagcattctg
    21541 gtttctgacg gtaaaacact gtggttctat aacccgttcg ttgagcaagc tacggcaact
    21601 tggctgaaag atgccaccgg taatacgccg tttatgctga ttgcccgcaa ccagtccagc
    21661 gactggcagc agtacaatat caaacagaat ggcgatgact ttgtcctgac gccgaaagcc
    21721 agcaatggca atctgaagca gttcaccatt aacgtgggac gtgatggcac aatccatcag
    21781 tttagcgcgg tggagcagga cgatcagcgc agcagttatc aactgaaatc ccagcaaaat
    21841 ggggctgtgg atgcagcgaa atttaccttc accccgccgc aaggcgtcac ggtagatgat
    21901 caacgtaagt agaggcacct gagtgagcaa tctgtcgctc gatttttcgg ataatacttt
    21961 tcaacctctg gccgcgcgta tgcggccaga aaatttagca cagtatatcg gccagcaaca
    22021 tttgctggct gcggggaagc cgttgccgcg cgctatcgaa gccgggcatc tgcattctat
    22081 gatcctctgg gggccaccgg gtaccggcaa aacaaccctc gctgaagtga ttgcccgcta
    22141 tgcgaacgct gatgtggaac gtatttctgc cgtcacctct ggcgtgaaag agattcgcga
    22201 ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc cgcactattc tttttgttga
    22261 cgaagtgcac cgattcaaca aaagccagca ggatgcattt ctgccacata ttgaagacgg
    22321 caccatcact tttattggcg caaccactga aaacccgtcg tttgagctta attcggcact
    22381 gctttcccgt gcccgtgtct atctgttgaa atccctgggt acagaggata ttgagcaagt
    22441 actaactcag gcgatggaag acaagacccg tggttatggt ggtcaggata ttgttctgcc
    22501 agatgaaaca cgacgcgcca ttgctgaact ggtgaatggc gacgcgcgcc gggcgttaaa
    22561 tacgctggaa atgatggcgg atatggccga agtcaatgat agcggtaagc gggtcctgaa
    22621 gcctgaatta ttgaccgaaa tcgccggtga gcgaagcgcc cgctttgata acaaaggcga
    22681 tcgcttttac gatctcattt ccgcactgca taagtcggta cgtggtagcg cacccgatgc
    22741 ggcgctctac tggtatgcgc gaattattac cgctggtggc gatccgttat atgtcgcgcg
    22801 tcgttgcctg gcaattgctt ccgaagatgt cggcaatgcc gacccacggg cgatgcaggt
    22861 ggcaattgcg gcctgggatt gctttactcg cgttggcccg gcggaaggtg aacgcgccat
    22921 tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc aacgctgtct acactgcgtt
    22981 taaagctgcg ctggcggatg ctcgcgaacg cccggattat gacgtgccgg ttcatttgcg
    23041 taatgcgccg acgaaattaa tgaaggaaat gggctacggg caggaatatc gttacgctca
    23101 tgatgaagca aacgcttatg ctgccggtga ggtttacttc ccgccggaaa tagcacaaac
    23161 acgctattat ttcccgacaa acaggggcct tgaaggcaag attggcgaaa agctcgcctg
    23221 gctggctgaa caggatcaaa atagccccat aaaacgctac cgttaatgtt atcgttgcgg
    23281 taatgttgtt actgtatccc tgtggtcgca ggctgtggcc acatctccca tttaattcga
    23341 taagcacagg ataagcatgc tcgatcccaa tctgctgcgt aatgagccag acgcagtcgc
    23401 tgaaaaactg gcacgccggg gctttaagct ggatgtagat aagctgggcg ctcttgaaga
    23461 gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa gcggagcgta actcccgatc
    23521 gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc gagcctttac gtctggaagt
    23581 gaacaaactg ggcgaagagc tggatgcagc aaaagccgag ctggatgctt tacaggctga
    23641 aattcgcgat atcgcgctga ccatccctaa cctgcctgca gatgaagtgc cggtaggtaa
    23701 agacgaaaat gacaacgttg aagtcagccg ctggggtacc ccgcgtgagt ttgactttga
    23761 agttcgtgac cacgtgacgc tgggtgaaat gcactctggc ctcgactttg cagctgcagt
    23821 taagctgact ggttcccgct ttgtggtaat gaaagggcag attgctcgca tgcaccgcgc
    23881 actgtcgcag tttatgctgg atctgcatac cgaacagcat ggctacagtg agaactatgt
    23941 tccgtacctg gttaaccagg acacgctgta cggtacgggt cagctgccga aatttgctgg
    24001 cgatctgttc catactcgtc cgctggaaga agaagcagac accagtaact atgcgctgat
    24061 cccaacggca gaagttccgc tgactaacct ggtgcgcggt gaaatcatcg atgaagatga
    24121 tctgccaatt aagatgaccg cccacacccc atgcttccgt tctgaagccg gttcatatgg
    24181 tcgtgacacc cgtggtctga tccgtatgca ccagttcgac aaagttgaaa tggtgcagat
    24241 cgtgcgcccg gaagactcaa tggcggcgct ggaagagatg actggtcatg cagaaaaagt
    24301 cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt tgcactggcg acatgggctt
    24361 tggcgcttgc aaaacttacg acctggaagt atggatcccg gcacagaaca cctaccgtga
    24421 gatctcttcc tgctccaacg tttgggattt ccaggcacgt cgtatgcagg cacgttgccg
    24481 cagcaagtcg gacaagaaaa cccgtctggt tcataccctg aacggttctg gtctggctgt
    24541 tggtcgtacg ctggttgcag taatggaaaa ctatcagcag gctgatggtc gtattgaagt
    24601 accagaagtt ctgcgtccgt atatgaacgg actggaatat attggctaat acccaatttt
    24661 tctgaatcta aaaagcgcct gcgggcgctt tttttgtctc cctttgatac cgaacaataa
    24721 ttactcctca cttacacgta atactacttt cgagtgaaaa tctacctatc tctttgattt
    24781 tcaaattatt cgatgtatac aagcctatat agcgaactgc tatagaaata attacacaat
    24841 acggtttgtt actggaatca atcgtgagca agcttgagtg agccattatg aaaacgaaaa
    24901 tccctgatgc ggtattggct gctgaggtga gtcgccgtgg tttggtaaaa acgacagcga
    24961 tcggcggcct ggcaatggcc agcagcgcat taacattacc ttttagtcgg attgcgcacg
    25021 ctgtcgatag cgccattcca acaaaatcag acgaaaaggt tatctggagc gcctgtacag
    25081 ttaactgtgg tagtcgctgc ccgctacgta tgcacgtcgt ggacggtgaa atcaaatatg
    25141 tcgaaacgga caataccggc gatgacaatt acgacggcct gcaccaggtt cgcgcctgcc
    25201 tgcgtgggcg ttccatgcgt cgccgtgtct acaatccgga ccgcctgaaa tatccgatga
    25261 aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat tagctgggaa gaagcctacg
    25321 acatcatcgc gaccaatatg cagcgcctga tcaaagagta cggcaacgag tctatctatc
    25381 tgaactatgg caccggtacg ctgggcggca ccatgacccg ctcctggccg ccgggaaata
    25441 ccctggtcgc gcggctgatg aactgctgcg gcggctatct gaaccattac ggcgactact
    25501 cctccgcgca aattgcggaa ggtttgaact atacctacgg cggctgggca gatggcaaca
    25561 gcccgtcgga tatcgaaaac agtaagctgg tagtgctgtt tggtaataac cctggcgaaa
    25621 cgcgaatgag tggcggtggg gtgacttact atcttgaaca ggcacgccag aaatctaatg
    25681 cccgcatgat catcatcgat ccgcgctata ccgacaccgg tgccgggcgc gaagatgagt
    25741 ggatccctat tcgtccggga acagatgccg cactggttaa cggtctggcg tacgtcatga
    25801 tcactgaaaa cctggtggat caggcattcc tcgataaata ttgcgttggc tacgatgaga
    25861 aaaccctgcc agccagtgcg ccgaaaaatg gccactataa agcttatatt ctgggtgaag
    25921 ggccagatgg cgtggctaaa acaccggaat gggcctcaca aatcaccggt gttccggcag
    25981 acaaaatcat caaactggct cgtgaaatcg gcagtaccaa accggcgttt atcagccagg
    26041 gatggggccc gcagcgtcac gctaacggtg aaatcgcaac ccgtgctatc tcgatgctgg
    26101 cgattctgac cggtaacgtt ggtattaatg gagggaacag cggcgcgcgt gaaggttcat
    26161 acagcttacc gtttgtccgt atgccgacct tggaaaaccc gatccagacc agcatttcga
    26221 tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat gacggcgctg cgtgatggtg
    26281 tacgcgggaa agataagctg gatgtgccga tcaaaatgat ctggaactat gccggtaact
    26341 gcctggttaa ccagcattct gaaatcaacc gtacccatga aatccttcag gatgataaga
    26401 agtgcgagct gattgtggtt atcgactgcc acatgacctc atcggcgaaa tatgctgaca
    26461 tcctgctgcc tgactgcacc gcttccgaac agatggactt tgcgctggat gcatcctgcg
    26521 ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa accgcgcttt gaatgtaaga
    26581 ccatctatga aatgaccagc gaactggcaa aacgtcttgg cgttgagcaa cagtttactg
    26641 aaggccgtac ccaggaagag tggatgcggc atctgtatgc ccagtcgcgg gaagcgattc
    26701 ctgaactgcc aacgtttgaa gagttccgca agcaggggat ctttaaaaag cgcgacccac
    26761 aagggcatca cgttgcttat aaagccttcc gtgaagatcc gcaggcaaac ccactgacta
    26821 cgccatcggg caaaattgag atttattcgc aggcgctggc tgacattgcc gctacctggg
    26881 aattgccaga aggcgatgtg atcgatccac tgccgatcta cacgccgggc tttgaaagtt
    26941 atcaggatcc gctgaacaaa cagtatccgc tgcagcttac gggtttccac tataaatctc
    27001 gcgttcactc aacttacggc aacgttgatg tgctgaaagc agcttgccgt caggaaatgt
    27061 ggatcaaccc gcttgatgcg caaaaacgcg gtatcaacaa cggcgataaa gtcaggatct
    27121 ttaacgatcg tggtgaagtt catattgagg cgaaagtgac gccacgaatg atgccaggtg
    27181 tggtcgcact gggtgaaggt gcctggtatg acccggatgc aaaacgtgtc gataagggtg
    27241 gttgtattaa cgtactgacc actcaacgtc cgtctcctct cgctaagggg aatccgtcac
    27301 atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac cgatgacaac ccagtatgga
    27361 ttttttattg attccagccg ttgcaccggt tgcaaaacct gcgagctggc ttgtaaagac
    27421 tacaaagatt tgacgccaga agtcagcttc cgccgcattt atgaatatgc tggcggcgac
    27481 tggcaggaag ataacggtgt ctggcaccag aacgtgtttg cctactatct gtcgatttcg
    27541 tgcaaccact gcgaagatcc ggcttgtact aaagtctgcc cgagcggtgc gatgcataaa
    27601 cgtgaagatg gttttgttgt ggtcgatgaa gatgtgtgca ttggctgccg ctactgccat
    27661 atggcttgcc cgtatggcgc accgcaatat aacgaaacga aaggccatat gaccaaatgc
    27721 gatggttgtt atgaccgtgt tgcggagggt aaaaagccga tctgtgttga atcttgtccg
    27781 ctgcgggcgc tggatttcgg ccctatcgac gagctgcgta aaaaacatgg cgatctggcg
    27841 gccgttgcgc cgttgccgcg agctcacttt accaaaccga atattgtgat caaacccaat
    27901 gccaatagcc gcccgaccgg ggataccact ggctatctgg caaacccgaa ggaggtgtga
    27961 gatgggaagt ggatggcatg aatggccgct gatgatcttc acggtcttcg ggcaatgtgt
    28021 ggcgggtggt tttatcgttc tggctttggc gctgctcaaa ggcgacctgc gagcagaagc
    28081 ccagcagcgt gttatcgcct gcatgtttgg cttatgggtg ctgatgggca ttggctttat
    28141 cgcttctatg ctccatcttg gttcaccgat gcgcgctttt aactcgctca accgggtagg
    28201 ggcttcagca ctcagtaacg aaatcgccag cggttcgatc ttttttgccg taggcggcat
    28261 cggctggttg ctggcaatgc tgaaaaagct gtcaccggca ttgcgtacgc tttggctggt
    28321 ggtgacgatg gttcttggcg tcgtctttgt ctggatgatg gtgcgtgtgt ataacagcat
    28381 tgataccgtt ccgacctggt acagcatctg gacgccgatg ggcttcttcc tgacgatgtt
    28441 catgggcggc ccgttactgg ggtacctgtt gctgagtctg gctggcgtcg atagctgggc
    28501 gatgcgtctg ctgccagcca tttccgtact ggcactggtg gtgagtggcg tggtgtcagt
    28561 gatgcagggc gcagagctgg cgactattca tagttctgtg cagcaggccg cagcgctggt
    28621 gccggactat ggtgcgctga tgtcctggcg gatcgtgctt ttggccgttg ccctgtgctt
    28681 gtggattgca ccacagctaa aaggttatca gcctgcggtt ccgctactat ccgtctcatt
    28741 cattctgctt ctggcagggg aattaatcgg tcgcggcgta ttctatggtt tgcatatgac
    28801 cgtggggatg gccgtcgcaa gctaatcagt aacaaccggg gtttcggccc cggttctctt
    28861 ttatttctgc ttcgttaacg tgtcataact ggtcatcaaa ttacgataat caggaatatg
    28921 gttagagaac aacgtcgcca gtccttcaat atcattacgc cagtcacggt gcagctcgca
    28981 agccacgccg aaccatgtca tcaattgcgc acctgcttgc gacatgcgat cccatgccga
    29041 atgccgggta atttcattaa aggtccctga cgcgtcagtc acaacaaaca catcaaaccc
    29101 ttcttcaatg gctgaaagcg ccgggaatgc cacgcaaact tcggttacca caccggcaat
    29161 aattaactgt tttttacctg tcgctttgac tgcttttaca aaatcttcgt tatcccaggc
    29221 gttaatattg cccgggcgag caatgtaagg tgcatcgggg aattgtgctt tcagttctgg
    29281 aactaatggg ccgttaggac cggtttcaaa actggtggta agaatagttg gtaagttgaa
    29341 atacttagcc aggtcaccca gcgccagcac gttattttta aacttatcgg gttcgatatc
    29401 ccgtacaagg gaaagtaaac cagcctggtg atcaacaagc aaaacagcag catcattttt
    29461 atcaagacga acatacggtt tggtcatcct cttctccttt cgagagtcag tggtacgcct
    29521 gaaatcggtc agacgtgatg cattgctctg aaagcataga cgggaaatat gagtttgctg
    29581 tgaccatgaa atttttcgac tgaacgaagc gtatgaaatt tgtgttagat caataaaaac
    29641 aatcagatga gattaatcat ctcttcagta ttagcgttat gaatgagtaa tataaatcga
    29701 taataccgcc gttgcgatat gcgcgtattt tcacccattg acaatgtttt tggcggtggc
    29761 atgatgcgca tgaaatttga acttcctcac ggttttaatt catgtccacg tatacccggc
    29821 ctgtcatgct tttgctgtct ggcctgcttt tgttgactct ggcgatcgcg gtgttaaata
    29881 cactcgtgcc gctttggctc gcccaggaac acatgtccac atggcaggta ggcgttgtca
    29941 gctcatccta ttttaccggc aaccttgtcg gtacattgct gacagggtat gtcattaaac
    30001 gcattggttt taaccgcagc tattatctgg cctccttcat ttttgccgct ggctgtgccg
    30061 gccttggcct gatgattgga ttctggagtt ggttggcgtg gcgttttgtc gcgggcgtcg
    30121 gctgtgccat gatttgggta gtcgttgaga gcgcgctgat gtgcagtggg acgtcacgta
    30181 accgtgggcg tttgcttgct gcatacatga tggtttatta cgtgggaacg tttttaggcc
    30241 agttactggt cagcaaggtc tcaaccgagc tgatgagcgt attgccgtgg gttacaggtt
    30301 tgacattggc agggatctta ccgctgttgt ttacgcgtgt cctgaatcag caggctgaaa
    30361 accatgattc gacgtcaatt acggcaatgc taaaactccg tcaggcgcgg cttggcgtga
    30421 atggctgcat tatctcagga atcgttctgg gatctctata tggcctgatg cctctgtacc
    30481 tcaatcacaa aggggtgagc aatgccagta ttggtttctg gatggcggta ctggtcagtg
    30541 cgggtatcct cggacaatgg ccgattggac gtctggcgga taagtttggt cgactgctgg
    30601 ttttgcgtgt tcaggtcttt gtcgtcattc tcggcagtat tgcgatgctt agccaggcgg
    30661 cgatggcccc agcgttattc atcctcggtg ccgctggctt tacgctatat ccggtggcga
    30721 tggcatgggc ttgcgagaaa gttgaacatc atcaactggt ggcgatgaac caagccttac
    30781 tgttgagcta tactgtggga agtctgcttg gcccgtcatt taccgctatg ctaatgcaga
    30841 atttctccga taatttattg tttatcatga tcgccagcgt atcgtttatc tatttgctga
    30901 tgctgctgcg caacgccggt catacgccga aacccgttgc tcacgtgtaa atgaattcaa
    30961 gcagagtgtg aacttactgt ttcacactct gcttttttgt ttcttctatc tgacttgctt
    31021 tattccaaat tttattcgtt taaaaataaa atgtgtagca ggttataatt ttgcatttcg
    31081 ctatttccgc acttcttatt tgccgcgcat aatccctcgt tttacagatg cccctataat
    31141 tttggcgaag gatttgtcta tggctgggaa tgttcaggaa aaacagctgc gatggtacaa
    31201 cattgcgctg atgtctttta tcactgtctg gggttttggc aacgttgtta ataactacgc
    31261 caaccagggg ctggtggttg ttttttcatg ggtgtttatc tttgcgctct atttcacacc
    31321 ttatgcgcta attgttggtc agttaggctc gaccttcaaa gatgggaagg gcggggtcag
    31381 tacctggatt aaacacacga tgggacccgg actggcttat ctcgccgcgt ggacctactg
    31441 ggtggtgcat attccctatc tggcacaaaa accccaggca attctgattg cgctcggttg
    31501 ggcgatgaaa ggcgacggtt cgctaatcaa agaatattca gtcgtagcgt tacaggggtt
    31561 aacgctggtg ctgtttatct tctttatgtg ggttgcttca cgcggtatga aatcgctgaa
    31621 aatcgtcggt tctgtggcag ggattgctat gtttgttatg tcgctcctgt atgtggcgat
    31681 ggcggtaacc gcgcctgcaa ttactgaagt gcatattgcg accacaaaca ttacctggga
    31741 aacgttcatt cctcatatcg actttaccta cattaccact atttcaatgc tggttttcgc
    31801 ggttggcgga gcagagaaga tttctcctta cgttaatcaa acgcgcaacc caggaaaaga
    31861 atttccaaaa gggatgttat gcctggcggt gatggttgcg gtttgtgcca ttctgggctc
    31921 gctggcgatg gggatgatgt ttgattcgcg taatatcccg gatgacttaa tgaccaacgg
    31981 tcagtattac gcctttcaga agctgggcga gtattacaac atgggtaata ctttaatggt
    32041 gatttacgcc attgcgaata ccctgggaca agtagcggcg ctggtattct cgattgatgc
    32101 cccgcttaaa gtgctattag gtgatgctga cagcaaatat attccagcca gtttatgtcg
    32161 taccaacgct tctggtacgc ccgttaatgg ctattttctg accctggtac tggtggcgat
    32221 tctgattatg ctgccgactc tcggcattgg tgatatgaac aatctctata aatggctgtt
    32281 gaaccttaat tcggtagtga tgccgctgcg ttatttatgg gtatttgttg catttattgc
    32341 agtcgttcgc ctggcgcaga aatataaacc agaatatgtc tttattcgta ataagcctct
    32401 ggcaatgacc gtcgggattt ggtgttttgc ctttaccgcc tttgcctgtt tgacggggat
    32461 cttcccgaaa atggaagcct tcactgcaga gtggaccttc cagttggcgc tgaatgttgc
    32521 aacgccgttt gtgctggtag gattaggact gatattcccg ctgctggcgc gtaaagcgaa
    32581 tagtaaataa ttattgtggt cggtgacgct gactctggca gatctgaccc gtttttacat
    32641 aatcgagaaa tgcgcgcaag ccgcaggaca tatactggcg gtttgggtaa tagatctgga
    32701 agcctggacg ctctgtgatc cagtcttcca gtacacattc taaacgaccg gtatcaaggt
    32761 actccttgat ctgttcatag agcagatacc cgataccagc acccatcaaa acggcatcca
    32821 gttcagcatc aacatca
//
LOCUS       DAZPLI010000030        32651 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZPLI010000030 DAZPLI010000000
VERSION     DAZPLI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32651
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.030"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            80..1582
                     /locus_tag="ACHUU3_001378"
     CDS             80..1582
                     /locus_tag="ACHUU3_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HFR2081495.1"
                     /translation="MNKKLYLFPDSNIFLQCKDLTQVNFSEITACDEVCIIITRPIQQ
                     EIDRQKGQGNSRLSKKARKAASLFNQVVDSTDMTLVIRERSPRVFLTMDLSLKPCEQL
                     SEQLDYQEADDRFVGIAAGYCADDPDSEVAIITNDSGPRFSAIKHNIICYKVPSSWLL
                     PAEPDEKDKQIKLLEAKLKQFTQSMPDFSIKVADDEQLNIVLPYYTALSEYEVDNLLC
                     QLTDAFPLVTNFDNEQTTPQSLEKIIKSFSQQEYFPVSADDISLYKEKHYPLWKKQCE
                     EILKNCHTHINPKAINYPLSFLLQNTGYTIAERAVVTFTAKGDFRLCGFNWEIMEELT
                     ESKRIDLPSAPTAPKGKWRSVCGVTERMPYAGGALETPLSSIISKEIGPLLSLTPTER
                     DKNDFYYKEKRNEPVQVVSLECEEWRHQVKEEIFPLFVYTANKPKKVNGAIEVRVDAN
                     NLTNPVIKTFKLKITIEEHSAFSTITEMVQTYIQEQKKRQKSSISAQIKT"
     gene            1826..2086
                     /locus_tag="ACHUU3_001379"
     CDS             1826..2086
                     /locus_tag="ACHUU3_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFR2081496.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEDIPWHDLPASHRGNNQMSLPLPQN"
     gene            2277..2417
                     /locus_tag="ACHUU3_001380"
     CDS             2277..2417
                     /locus_tag="ACHUU3_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR2081497.1"
                     /translation="MSQKSLTTITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(2724..3023)
                     /gene="ihfA"
                     /locus_tag="ACHUU3_001381"
     CDS             complement(2724..3023)
                     /gene="ihfA"
                     /locus_tag="ACHUU3_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR2081498.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(3028..5415)
                     /gene="pheT"
                     /locus_tag="ACHUU3_001382"
     CDS             complement(3028..5415)
                     /gene="pheT"
                     /locus_tag="ACHUU3_001382"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR2081499.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(5430..6413)
                     /gene="pheS"
                     /locus_tag="ACHUU3_001383"
     CDS             complement(5430..6413)
                     /gene="pheS"
                     /locus_tag="ACHUU3_001383"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR2081500.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(6622..6746)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(6696..6740)
                     /gene="pheM"
                     /locus_tag="ACHUU3_001384"
     CDS             complement(6696..6740)
                     /gene="pheM"
                     /locus_tag="ACHUU3_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR2081501.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(6863..7219)
                     /gene="rplT"
                     /locus_tag="ACHUU3_001385"
     CDS             complement(6863..7219)
                     /gene="rplT"
                     /locus_tag="ACHUU3_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR2081502.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(7272..7469)
                     /gene="rpmI"
                     /locus_tag="ACHUU3_001386"
     CDS             complement(7272..7469)
                     /gene="rpmI"
                     /locus_tag="ACHUU3_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR2081503.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(7566..8108)
                     /gene="infC"
                     /locus_tag="ACHUU3_001387"
     CDS             complement(7566..8108)
                     /gene="infC"
                     /locus_tag="ACHUU3_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR2081504.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(8112..10040)
                     /gene="thrS"
                     /locus_tag="ACHUU3_001388"
     CDS             complement(8112..10040)
                     /gene="thrS"
                     /locus_tag="ACHUU3_001388"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR2081505.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            10565..12463
                     /gene="espL1"
                     /locus_tag="ACHUU3_001389"
                     /pseudo
     CDS             10565..12463
                     /gene="espL1"
                     /locus_tag="ACHUU3_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            12635..12742
                     /gene="yniD"
                     /locus_tag="ACHUU3_001390"
     CDS             12635..12742
                     /gene="yniD"
                     /locus_tag="ACHUU3_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR2081506.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(12795..13553)
                     /locus_tag="ACHUU3_001391"
     CDS             complement(12795..13553)
                     /locus_tag="ACHUU3_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR2081507.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQLLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            13840..14769
                     /gene="pfkB"
                     /locus_tag="ACHUU3_001392"
     CDS             13840..14769
                     /gene="pfkB"
                     /locus_tag="ACHUU3_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR2081508.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            14870..15160
                     /gene="ghoS"
                     /locus_tag="ACHUU3_001393"
     CDS             14870..15160
                     /gene="ghoS"
                     /locus_tag="ACHUU3_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR2081509.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            15266..16126
                     /locus_tag="ACHUU3_001394"
     CDS             15266..16126
                     /locus_tag="ACHUU3_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR2081510.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(16167..16703)
                     /locus_tag="ACHUU3_001395"
     CDS             complement(16167..16703)
                     /locus_tag="ACHUU3_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR2081511.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            16850..17518
                     /gene="hxpB"
                     /locus_tag="ACHUU3_001396"
     CDS             16850..17518
                     /gene="hxpB"
                     /locus_tag="ACHUU3_001396"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR2081512.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            17681..18271
                     /locus_tag="ACHUU3_001397"
     CDS             17681..18271
                     /locus_tag="ACHUU3_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR2081513.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            18404..19795
                     /gene="tcyP"
                     /locus_tag="ACHUU3_001398"
     CDS             18404..19795
                     /gene="tcyP"
                     /locus_tag="ACHUU3_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFR2081514.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(19799..20603)
                     /locus_tag="ACHUU3_001399"
                     /pseudo
     CDS             complement(19799..20603)
                     /locus_tag="ACHUU3_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20892..21155)
                     /gene="cedA"
                     /locus_tag="ACHUU3_001400"
     CDS             complement(20892..21155)
                     /gene="cedA"
                     /locus_tag="ACHUU3_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR2081515.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            21338..23599
                     /gene="katE"
                     /locus_tag="ACHUU3_001401"
     CDS             21338..23599
                     /gene="katE"
                     /locus_tag="ACHUU3_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR2081516.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(23857..24615)
                     /gene="chbG"
                     /locus_tag="ACHUU3_001402"
     CDS             complement(23857..24615)
                     /gene="chbG"
                     /locus_tag="ACHUU3_001402"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR2081517.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(24628..25980)
                     /gene="celF"
                     /locus_tag="ACHUU3_001403"
                     /gene_synonym="chbF"
     CDS             complement(24628..25980)
                     /gene="celF"
                     /locus_tag="ACHUU3_001403"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR2081518.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(26085..26927)
                     /gene="chbR"
                     /locus_tag="ACHUU3_001404"
     CDS             complement(26085..26927)
                     /gene="chbR"
                     /locus_tag="ACHUU3_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFR2081519.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(26935..27285)
                     /gene="chbA"
                     /locus_tag="ACHUU3_001405"
     CDS             complement(26935..27285)
                     /gene="chbA"
                     /locus_tag="ACHUU3_001405"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR2081520.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(27336..28694)
                     /gene="chbC"
                     /locus_tag="ACHUU3_001406"
     CDS             complement(27336..28694)
                     /gene="chbC"
                     /locus_tag="ACHUU3_001406"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFR2081521.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(28779..29099)
                     /gene="chbB"
                     /locus_tag="ACHUU3_001407"
     CDS             complement(28779..29099)
                     /gene="chbB"
                     /locus_tag="ACHUU3_001407"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR2081522.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(29398..29736)
                     /gene="osmE"
                     /locus_tag="ACHUU3_001408"
     CDS             complement(29398..29736)
                     /gene="osmE"
                     /locus_tag="ACHUU3_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR2081523.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            29938..30765
                     /gene="nadE"
                     /locus_tag="ACHUU3_001409"
     CDS             29938..30765
                     /gene="nadE"
                     /locus_tag="ACHUU3_001409"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR2081524.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREADIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            30995..31882
                     /gene="cho"
                     /locus_tag="ACHUU3_001410"
     CDS             30995..31882
                     /gene="cho"
                     /locus_tag="ACHUU3_001410"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR2081525.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(31842..32417)
                     /gene="ves"
                     /locus_tag="ACHUU3_001411"
     CDS             complement(31842..32417)
                     /gene="ves"
                     /locus_tag="ACHUU3_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR2081526.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         8659 a         8095 c         7433 g         8464 t
ORIGIN      
        1 tggtaagaat catgctttga taaccaattg ataactatta ttatattaat ctaacaccat
       61 tgacatagtt gaggtttgaa tgaacaaaaa gctgtattta ttcccagact caaacatttt
      121 tctacaatgc aaggacttga cgcaggtaaa tttttccgag attactgctt gtgatgaagt
      181 atgtatcatt attacaaggc ccattcagca agagattgac cgccagaaag ggcaaggtaa
      241 ttcccgccta tccaaaaaag caaggaaggc tgctagccta tttaatcaag tagttgattc
      301 aactgacatg actcttgtta ttcgtgaacg ctcgccgcga gtttttctta ccatggatct
      361 aagcctgaaa ccatgtgaac agttatctga acaattggat tatcaggagg ccgacgatcg
      421 ttttgttggt attgcggcag ggtattgcgc tgacgatcca gattcggagg tggcaatcat
      481 taccaatgat agcggtccac gtttttcagc aataaaacat aatataatct gctataaagt
      541 gccttcttca tggttgcttc ctgctgagcc agatgaaaag gataaacaaa ttaaattgct
      601 ggaagctaag ctgaagcaat tcactcagtc tatgcccgat ttcagcatta aagtagccga
      661 tgacgagcaa ttaaatattg tacttcctta ttatacagct ttatctgaat atgaagttga
      721 taatttgctt tgtcaactaa cggatgcatt cccattggtt acaaactttg acaatgaaca
      781 aactacccca caatcactag aaaaaatcat caagtcattt agtcaacagg agtatttccc
      841 tgtatcagca gatgatattt ctctttataa agaaaaacat tatccacttt ggaaaaagca
      901 gtgtgaagaa atactgaaaa actgtcatac tcatattaat ccgaaggcaa taaattaccc
      961 gctgtcgttt ttattacaaa acacaggtta tactatcgct gaacgtgctg tggtaacatt
     1021 tacagcaaaa ggagattttc gtctgtgtgg atttaattgg gagataatgg aagagctgac
     1081 tgaaagcaaa aggattgacc taccttcagc ccctaccgcc cccaaaggaa aatggcgtag
     1141 tgtatgtggc gtaacagaaa gaatgcctta cgctggggga gctttggaaa caccgctatc
     1201 cagtataatt tcaaaagaaa ttggtccttt gttatctctt actccaacag aaagggataa
     1261 aaacgatttt tattataaag aaaagagaaa tgagccagtt caagttgtat cgctggaatg
     1321 cgaagagtgg cgtcatcaag tcaaagaaga aatattcccg ttgttcgtat atacagccaa
     1381 caaaccaaag aaagtgaatg gtgcaattga ggtgagagtt gatgcaaata accttaccaa
     1441 tccggtcatt aaaacattta aattaaaaat aactatagaa gaacatagtg cattttcaac
     1501 cataacagaa atggtgcaga catacattca agagcagaag aaaagacaga aaagttctat
     1561 tagcgcacaa atcaaaacat gagggtgtac tcaaataata caatgctata gtaaatctat
     1621 aagtgcataa ctatttcctg atcttttccc aaaatagatg acaggataaa aaacattcca
     1681 atcttttaca ggctttggat aaatcgtatc aaagcctatt tcttcaagag ttaaatcacc
     1741 gcttttcgct cgaaacggac tggctgttgg agtatgagcc agaacaactt agaatagccc
     1801 ctgcactacg ttaagggagg tcgctatgtt ccgttgtccg ctttgtggcg catctgcccg
     1861 tatccgcacc agtcgtccgg aaaatgattc aaacaccgtg cggcaaaagt attaccagtg
     1921 taacaatctg gaatgcggcg tatgcttctc aacactggaa gctttccata aattcacatc
     1981 aaaacacgcc tccggcgttc actcttcaga agatatcccg tggcatgatc tgccagcttc
     2041 acacagggga aacaatcaga tgagcttgcc tttacctcag aattaacagg cagaattgcc
     2101 ggagtaacaa aaaagcgata gattacgcgc gggtgccttt cggctgatgg tcggagggaa
     2161 tacccgaagg ccagatgtgg aaaggccccg gaaaacatct ctgtttaacc gaggccctaa
     2221 ccgcattacc ttgacaagtg aaaggttagc gcctctccgg aaaaggagca agtgctatgt
     2281 cgcaaaaatc gcttacgacc atcacgttct gcgtgacggc aatcctcatc atctggatgt
     2341 tgcacggttc gctgtgtgaa atacggatga gcttctgggg agcggagttt gcggcgttct
     2401 tacagtgtaa gcagtaagga aaccgcgacg ggggagcaat cccccgtcaa tcggttgcca
     2461 gggtaaggcc ggataaggca ccctatctca cagatatgaa caacaaaccc gcagcgtaaa
     2521 aactgcgggt tttctttttg gtgccctcac cgacttgaaa cagtgataag actataagac
     2581 aaagcccgca atgtagcggg cttactcccc tcaccctcca ctgaatgtgg acacacagtg
     2641 gaacgaagcg gcataagata ttataaatca atcagttatt gaaagaaaaa aggccgcaga
     2701 gcggcctttt tagttagatc agattactcg tctttgggcg aagcgttttc gacccggctt
     2761 tttaacttct gcccgggtct gaaggtcacc acgcgccgtg ctgtaatggg aatatcctcg
     2821 cccgttttcg ggttacgtcc cgggcgttga ttcttatcac gcagatcgaa gttaccaaaa
     2881 ccagagagtt tcacctgttc gccgttttcc agagcgcgac ggatctcttc gaaaaacagt
     2941 tcaaccagtt ctttggcatc ccgcttgcta agcccaagct tatcaaacag atattctgac
     3001 atttcagctt ttgtaagcgc cataggttca atccctcaat gatgcctgga atcgctcttt
     3061 taatgcctct acacatttgg cgacggtagc ggcaatctcc tcttcttcga gtgtacggct
     3121 ggtatcttgc aggatcaggc ttatggcgag gctcttatac ccctccgcaa cacccttacc
     3181 gcggtacacg tcaaataagt ttacgccaac tacctgattt acgccaactt tcttacattc
     3241 ggataaaata tccgctgcgg gaacgttttc tgcgaccacc accgcgatgt cacgacggtt
     3301 cgccgggaag cgagaaatct cgcgcgcctg aggcaccacg cggtctgcga gcttgttcca
     3361 ctccagttcg aacaccagag tgcgaccgtt aagatccagt ttacgttcca gttcaggatg
     3421 aacaactcca acaaaaccaa tacgttcccc tttcagataa atcgctgcgg attgccccgg
     3481 atgcagtgcc ggattcgctt ctgcacggaa ctcaacctca ttcagtttac cggtcaggtc
     3541 gagaacggat tcaagatcgc ctttcaaatc atagaaatca acggtctctt ttgccaggtt
     3601 ccagtgctct tcgtaacggt taccgcaaat cacaccggct aacatcagat cctgacgaat
     3661 gcccaacggt gcctgagtat ctggtacgaa acgcagaccg ctttcgaaaa tgcgcacacg
     3721 gttctgctga cggttctggt tgtacaccac ggttgccagc aggccggtcc acagagaaag
     3781 acgcattgct gacatttcaa cagagatcgg gcttggcagc agtaaggctt caacgcctgg
     3841 atggatcatc tgctgcactt tcggatcaac gaagctgtag gtgatcactt cctgatagcc
     3901 tttgtcgttg agcagcgttt tcacgcgctt gagcgacagg tcagcttcac ggtgagtacc
     3961 cataatcagg cttgcctgta ccggctcatc cgggatgttg ttgtagccgt aaacacgcgc
     4021 gacttcttca accagatctt cttcaatctc catatcgaaa cgccagctcg gcgcaactgc
     4081 ctgccactcg tctttgcctt cggtcacttc gcagccgaga cgacgcagaa tgtcagttac
     4141 ctgttcatca gcaatatgat ggccgatcag gcgatccagt ttgctacgac gtaaagtgat
     4201 ggttgcacgc ttcggcagcg ttgcttcgtt ggtgatatca attaccggac cagcctcacc
     4261 accgcagatg tcgatcagca gacgggtcgc acgttccatc gctttgtgct gcagtgccgg
     4321 atcaacgcca cgctcataac ggtgagacgc atcagtatgc aggccatgac gacgagcacg
     4381 accggtgata gacagcgggc tgaagaaagc gcattccagc aagacgtttt gcgtttcgtc
     4441 attcacgcca gagtgttcgc caccaaagat gccgcccatc gccagcgcct tgttgtggtc
     4501 ggcgatgacc agagtgtcag cattcagctt cgcttcagtg ccatcaagca gcaccagcgt
     4561 ttcgccctct tttgccatcc gcaccacaat gccgccttca atgcgatctt tatcgaaagc
     4621 gtgcatcggc tggcccagtt cgagcagtac atagttggtg acgtcaacaa ctgcatcgat
     4681 agaacggatc ccgcaacgac gcagtttttc tttcatccac agcggagttg gcgctttaac
     4741 gttaatgcct tttaccacac ggccaagata gcgcgggcag gcttcaggcg cttcgactgt
     4801 aatcggcaac gtgtcgtcga tggtcgcacc aaccggaacg atttccggtt caaccagcgg
     4861 cagctgattc agcacggcaa cgtcacgcgc aacaccaatg atacctaagc agtcggcacg
     4921 gtttggcgtt acgctgattt cgatggtgtt gtcatcgagt ttcaggtatt cgcggatgtc
     4981 agtgccaatc ggcgcatctg caggcagttc gataatgccg ctgtgatcgt cagaaattcc
     5041 cagttcagag aaggagcaca gcatcccttc agacggttcg ccacgcagtt tcgccgcttt
     5101 aattttgaaa tcacccggca gaacagcacc aatggtagct accgccacac gcaggccctg
     5161 acggcagttt ggcgcaccac agacgatgtc cagcaggcga tcgccgccga cattcacttt
     5221 tgtcacacgc agtttgtcag cgttcggatg ctgcgcacac tcaaccactt caccaacgac
     5281 cacgccgtgg aagctaccgg caaccggttc tacaccgtca acttccaggc ccgccatagt
     5341 gatttgattc gccagcgcat cgctatcaat cgccgggttc acccattcgc gtaaccacag
     5401 ttcactgaat ttcataatct attcctgcct tatttaaact gtttgaggaa acgcagatcg
     5461 ttttcgaaga atgaacgcag gtcggtgacg ccgtaacgca acatagtcag acgctccatc
     5521 cccatcccga aggcgaaacc agagtaaact tccgggtcga tgccaacgtt acgcagcacg
     5581 ttcggatgca ccatcccgca acccagcact tccagccatt taccgttttt acccatgacg
     5641 tcaacttctg cagaaggctc ggtgaacggg aagtaggaag gacggaagcg aatctgcaaa
     5701 tcttcctcaa agaagttacg caggaaatcg tgcagagtgc ctttcaggtt ggtaaagctg
     5761 atgttggtat caacaatcag accttccatc tgatggaaca tcggcgtgtg agtctggtcg
     5821 tagtcgttac gataaacgcg gccaggcgcg atgatacgaa tcggtggctg ctgggctttc
     5881 atggtgcgga tctgtacgcc agaggtctgg gtacgcagca ggcgggtagt gtcaaaccag
     5941 aaagtgtcgt ggtcagcgcg cgccgggtgg tgaccaggaa tgttcagagc atcgaagtta
     6001 tgataatcgt cttcgatttc cggcccggtt gccacggtaa agccaagctc accgaagaaa
     6061 ctttcgatac ggtcgatggt acgggtaacc ggatgcagac cgccgttttc aatgcgacga
     6121 cctagcaggg agacatcaat cgtttccgcc gccagacgcg cattcagcgc tgcgctttcc
     6181 agttccgctt tacgcgcatt cagcgcctgc tgaacctgct ctttcgcttc gttgataacc
     6241 gcaccagctg ccggacgctc ttctggcggc agctcacgca gggtcgtcat ctgaagggtt
     6301 aagtgccctt ttttacccaa atattcgacg cgcacattat ctaacgcggc aacatctgac
     6361 gcctggctaa tggccgcctt cgcactggca accagttctg cgagatgtga catggttttc
     6421 ctcattgtgt cagtggtgac actggttcgt tggacttaga gcctatccca tcaggctatt
     6481 ttacttgcca ttttggtccc gggcagtgct cgaaattctc acgtacttaa atacgctccg
     6541 gtttctccgc gctggccgtg tccagactgg ctgcgacaat tacgcctgat agactggctt
     6601 tttatttttc aaaacgcgca tacaaaaaaa gcctccactg ggaggctttc aggcgctgtt
     6661 ttccgtttct cttctcacgc gctagcctcc tggattcagg tgctaaagta aaaaaagaag
     6721 cggaaaatag cagcattcat tgcttgcgtt accttttggt actcttcaaa agacctttat
     6781 tgaaaaggct acggcgataa aagtcaatgt tttgatggcg ttgaaacgaa aagagggaga
     6841 ctagctccct ctttcaactg gcttatgcca gagctgcttt cgctttttca accagagcgg
     6901 tgaacgctac tttgtcgaat actgcgatat cagccaggat cttacggtcg atttcaacag
     6961 aggctttttt caggccattg atgaatttgc tgtaagaaat accgttctga cgtgctgctg
     7021 cgttgatacg cgcaatccac agttgacgga actgacgctt acgttgacga cggtcacggt
     7081 aagcatactg accagctttg ataacagcct ggaaggcaac gcggtatacg cgagaacgcg
     7141 caccgtagta gcctttagct tgtttcaaaa ttttcttgtg acgtgcgcgt gcaataacac
     7201 cacgttttac gcgagccata tgtgctctcc tgtatctata ttctaattaa aaagttaaaa
     7261 acgttaacgg cttatgcgta cggcaggcac gcgattacca ggcccagatc gcctttggaa
     7321 accatggctt tcggacgcag gtgacgttta cgtttggtcg cttttttggt cagaatgtga
     7381 cgcaggttag cgtgcttgtg cttaaaacca cctttaccgg tttttttgaa gcgcttagca
     7441 gcaccgcgta cggtcttaat ttttggcatt ttaataactt ccacttcgca ttgttaataa
     7501 acgaaacaaa ggcgaacaaa gcctgtgaag cccgaaggct ccacagacag tgctacttga
     7561 aggccttact gtttcttctt aggagcgagc accatgatca tctggcggcc ttcgatcttc
     7621 gttgggaagg attcgaccac tgccagttct tgcaaatcgt ctttcacgcg attaagcact
     7681 tccataccga tttgctggtg cgccatctca cgaccgcgga aacgcagcgt gattttggct
     7741 ttatcaccct cttcgagaaa gcgaatcagg ctgcggagtt ttacctgata gtcgccttca
     7801 tctgtaccag gacggaattt aatttcctta acctggataa ctttttgctt tttcttctgt
     7861 tccttagaag acttgctctt ttcatagagg aatttgccgt aatccattat acgacaaacc
     7921 ggcggctcgg cgttagggct gatctcgact aagtctactc cggcttcttc tgctttctcc
     7981 agagcttctc tcagactcac aataccaagc tgctcgcctt ccagacctgt taagcgaact
     8041 tcctgggcgc gaatttcgcc attgatacgg ttagggcgcg ccgtttgaac tcgttttccg
     8101 cctttaatac cttattcctc caattgttta agactgcggc tgcgaatctc ttgttgcagc
     8161 ttctcgatca cttcatttac gtccatgctt cccaggtctt taccacggcg ggtgcgaacg
     8221 gcaactttgc ctgattccac ctctttatca ccacagacca gcatatatgg gacgcgacgc
     8281 aaagtgtgct cgcggatttt aaagccaatc ttctcatttc tcaagtctgc tttaacacga
     8341 atgcccgcat ttgatagttt ttgcgtcaat tcgttaacgt attcagactg tgaatcggta
     8401 atattcatga taacaacctg aactggcgca agccaggtcg ggaagaaacc agcgaattct
     8461 tcggtcagga taccgatgaa acgttccatc gaccccagaa ttgcgcggtg aatcattacc
     8521 ggtaccttac gctcgttgtc ttcgcccaca taggaggcgc tcagacgaga cggcaaggag
     8581 aagtccagct gtactgtacc gcactgccat gcacgatcga ggcagtcata cagggtaaat
     8641 tcaattttcg gaccgtagaa agcgccttca cccagttgat attcaaacgg gatgttgttt
     8701 tcttccagcg caaccgccag gtccgcctca gcacgatccc acatttcgtc gctgccaata
     8761 cgtttttcag gacgagtgga gagtttgacg acgatcttct cgaagccaaa agtgctgtac
     8821 atatcataga ctaaacggat acatccgtta acttcatcgc gaatttgttc ttcagtacag
     8881 aagatatgcg cgtcatcctg ggtaaatcca cgcacgcgca tcaggccatg cagcgaacct
     8941 gacggctcgt tacggtggca gctaccaaac tcggccatac gcagcggcag atcgcgataa
     9001 gacttcagcc cctggttgaa aatttgtacg tgacccgggc agttcatcgg cttaatgcag
     9061 tattcacggt tctcagaaga tgtggtgaac attgcatctt tgtagttgtc ccagtgaccg
     9121 gttttttccc acaggacacg gtccatcatg aacggacctt taacttcctg atactggtac
     9181 tctttcagtt tagaacgaac aaacacttcc agttcacgga agatggtcca gccgtcgttg
     9241 tgccagaata ccatacccgg cgcttcttcc tgcatatggt acaggtcgag ctgtttaccg
     9301 attttacggt ggtcgcgttt cgcggcttct tccaggcgct gcaggtaagc gttaagtgct
     9361 tttttgtctg cccacgccgt accgtaaata cgttgcaaca ttttgttgtt gctgtcgcca
     9421 cgccagtaag cccctgccgt tttcattagt ttgaaatgat ggcagaaacg catgttcggt
     9481 acgtgcggac cgcggcacat atcgacatat tcttcatgga agtacagacc tggcttgtca
     9541 tcatgggcga tgttttcgtc aagaatggag actttgtagc tctccccacg gttggcgaaa
     9601 gtttcacgcg cttcgtgcca gctgactttc ttcttaatga cgtcgtagtt tttctcagca
     9661 agctcatgca tccgcttctc gagtgcttcg acatcttcct gggttaacgt gcggtcaaga
     9721 tcaacgtcgt aataaaaacc gttgtcaata accgggccga ttgccatttt ggtatgcggc
     9781 caaagttgtt taatcgcatg ccctaacagg tgcgcacagg agtgacgaat gatctccaga
     9841 ccttcttcgt ctttggcggt aatgatcgac agttgtgcgt cgttttcaat cagatcgcaa
     9901 gcatcaacca gttcgccatt aacgcgccct gcgatacagg ctttcgccag acctggacca
     9961 atgtccagcg caacatccat ggggcttaca gcgtggtcgt aatggcgttg gctgccatca
    10021 ggaagagtta taacaggcat tttatatcct taatttgcag tggtgaccca cacgaaagat
    10081 cacatacaaa gaaaaatttg tttattaaca gttcattgcg aaaccatcta gccaacaaat
    10141 gctaaattgg ttcgcaatca ggtacacaaa tgaaagacat taacctctca ccttccaccc
    10201 ggttgaacga tgttaacact aaaaaaaggg agattgtacc tttccgtttc acattgattc
    10261 gattcgcaat tcgtttgtag tgagtacacc agcaaaacgc ttaaagaacc actgataaca
    10321 tcacggtgcg tctgaaaggt ttactgcgag cggatttaca gcagcataaa ctgaatccag
    10381 caaagttcgt tgcgtgattt ggcggtagta ttttaaacgc tcatgagatt aatgacgaag
    10441 tggtcatatc acaatgataa aagtgacaca attcttatag caatttttcg tgcacatttc
    10501 gttctggcga taataattaa tcatcatcct caaaacctcc agacatatat aaggcgaata
    10561 gattatgagt caaaacgata tcattatcag aacccattat aagtctcctc atagattgca
    10621 catcgatagc gacataccaa cgccttcatc agagcctatt aatcaatttg cgcgccaact
    10681 catcacccta cttgatacct ctgacttaag ttcgatgctg tcatactgtg ttactcagga
    10741 atttaccgca aactgtcgaa aaatttcaca aaattgttat tccactgccc tttttatcat
    10801 taactttgcc acttcaccca tccatgcaga aaatatactc attacattac actataaaaa
    10861 agaaatcatt cccttatttc tggaaaccac gcctattaag gctaaccatt tgcgaagcat
    10921 actggattat attgaacagg aacagttaac tgccgaagat cgtaaccatt gtatgaaact
    10981 gtctaaaaaa atccatagag aaaaaactat acaaccaaca gtaaatctca atggtagtgc
    11041 atttttttcg caatctcctt ctgacgctat tttttgtcgc catctgtcat tgcaatacgc
    11101 ccttgattca ttgagaaatg gaaaaggcaa agtcaacctg attaaacact actcctccgt
    11161 tgaatccata cagcagcatg tccccttagt ccgggacgcg gagttcagag cattacttcg
    11221 ccatcctcct gcagggagtc gcgttatcgc gagtaaggat tttggcttcg ctttagatat
    11281 tttcttttgt cgaatgatgg caaacaatgt cagtcatatg tccgcgattt tatatataga
    11341 caatcatact ttgtcagtaa ggctacgaat aaagcagtca gtgtatggac aattaaatta
    11401 tgttgtgtct gtttacgacc cgaacgatac caacgttgcc gtcagaggca cccacagggc
    11461 agcacggggc tttctctcgc ttgataagtt catcagttca gctcccgatg ctcagacctg
    11521 ggctgatagg tatgttcgca actgtgcaat tgctattctt cctctattac ctgagggagt
    11581 tccagttgct attttagcgg gtatcacgac acgaatgcca tttgccccta tacatccatc
    11641 ggcaatgttg ttaataatgg ccacaggcca gactcaatag cttattacac tattcaaaca
    11701 gttgcccata ctccctgaaa aagaaatcat tgaaataata actgcgcaga atagcgttgg
    11761 tacacctgct ttatttctgg ctatgatgaa cggacaaact gacaacgtaa aaatatttat
    11821 gcaagaaatt cagtcactgg tatacaatca tatcattcat gaagataatc tggttaaatt
    11881 actgcaaact aaaagtgcta acgaaacacc tggactctat atctccatgt tgtatggatt
    11941 cgatgaaata atcgatatct ttctgaatgc attaaccact ccaatagcac aagaactttt
    12001 aaacaaaaaa atggtgatga gtattttagc catgaaaata catgatggtg agccaggatt
    12061 atacgccgca atggaaaata atcacccttt gtgtgtcaca cggttcctct ctaaaattaa
    12121 tggcatcgcc tttaaataca agttgagcaa agctaacatc atggatttat taaaaggagc
    12181 tacagcacag ggaacccctg cattatacat cgccatgagc aagggtaatg aagacgtcgt
    12241 gttatcttat atatcgacgc tgggtgcttt tgcaaaaaaa cattctttta gtcaacatca
    12301 gttatttaca ctattggccg ctaaaaatca tgacaacatg tcagctgttc atatagccat
    12361 tcatcataag cattataaaa ctgtagaaac atattatgct gctataaatg tcatcagcca
    12421 aagcctgagt tttagtgctg atgaaataaa gacgtattta taaccagcta tttacgatat
    12481 atatcgatac ataattttat ttttttcata aaacgcagaa acagtgacca tgtgcacaac
    12541 gccacccgac ggctgtaata acaaaaaatc ccttgatgcc tatccctttt gttacactcc
    12601 gttatcacgc acaagagata tgcaggacac tggtatgccg actaaacgct ttgataaaaa
    12661 acactggaag atggtggtgg tgctactggc aatctgtggc gctatgttgt tgctacgttg
    12721 ggcagcaatg atttggggct gagcagtgac aaacgaagac agccagaccg cataacacgg
    12781 tccggcagat aattttacat gctgtagccc agcgataagg ttgtccgacg atcggtatgt
    12841 tccggtgcag actctggagg ttcagagttc catgtgacgt tgtaagccac cttcagtcca
    12901 aaatgttcat taatagcaac atttaatgcg ctttcggagt tcagcgttgt atcttccgcg
    12961 ccaaagaccg aaacaccctg cgtaaattta gcgttgtcag tcaactgcca tgcataagcc
    13021 ccggaggcat agcccagcag ctgggtttca ctggcattgt cggtatattt gtcgtaacgc
    13081 acacctgggc cgaattcaaa gcggaaactg tgtaccgggc cattcaaaaa ctggcgacca
    13141 taacccgcgg tcaacacatc gcgctctcga tagccgttat aacggtctgt cagccagctt
    13201 gcctgaccaa ataaatagtc ataatcagtt aaattaaaac ggctacgccc gcccgccgca
    13261 tatttttctg aagaacgctc atcattagaa gaagtattac tggcgttccc ccacagcgac
    13321 caggcagtgg tttgtccata ccaggtcatg gtggtgtcag ccgtaagtga ggagcttttc
    13381 gtattgcctg attgtgcaag atatcctgcg ttcagattac cttcgaaagg ttttttagcg
    13441 ctggcaggat cgtccatgac agtaaaaacg gaatcatcgg cagctgcatt cagtgacgca
    13501 aacatgcccc ccgccaacat aacgatggca ggaactgtct tcaaaagctt catttatcaa
    13561 gagtccgtac aacaaaaaaa gagaccatcg cggtcccgga aactttctta aggatcaaag
    13621 attagcgtcc ctggaaaggt aacgaattat aaaaaggcgc gaataactta gcaatgtatt
    13681 cttatttcat tttttgaata agcatgtggc gaaaacagat ttttatttat atatatttat
    13741 ctgcaaaatt ttaaataaag ctccaataaa tcatattgtt aatttcttca ctttccgctg
    13801 attcggtgcc agactgaaat cagcctatag gaggaaatga tggtacgtat ctatacgttg
    13861 acacttgcgc cctctctcga tagcgcaaca attaccccgc aaatttatcc cgaaggaaaa
    13921 ctgcgctgta ccgcaccggt gttcgaaccc gggggcggcg gcatcaacgt cgcccgcgcc
    13981 attgcccatc ttggaggcag tgccacagcg atcttcccgg cgggtggcgc gaccggcgaa
    14041 cacctggttt cactgttggc ggatgaaaat gtccccgtcg ctactgtaga agccaaagac
    14101 tggacccggc agaatttaca tgtacatgtg gaagcaagcg gtgagcagta tcgttttgtt
    14161 atgccaggcg cggcattaaa tgaagatgag tttcgccagc ttgaagagca agttctggaa
    14221 attgaatccg gggccatcct ggtcataagc ggaagcctgc cgccaggtgt gaagctggaa
    14281 aaattaaccc aactgatttc cgcttcgcaa aaacaaggga tccgctgcat cgtcgacagt
    14341 tctggcgaag cgttaagtgc agcactggca attggtaaca tcgagttggt taagcctaac
    14401 caaaaagaac tcagtgcgct ggtgaatcgc gaactcaccc agccggacga tgtccgcaaa
    14461 gccgcgcagg aaatcgttaa tagcggcaag gccaaacggg ttgtcgtttc ccttggtcca
    14521 caaggagcgc tgggtgttga tagtgaaaac tgcattcagg tggtgccacc accggtgaaa
    14581 agccagagta ccgttggcgc tggtgacagc atggtcggcg cgatgacact gaaactggca
    14641 gaaaatgcct ctcttgaaga gatggtccgt tttggcgtag ctgcggggag tgcagccaca
    14701 ctcaatcagg gaacacgtct gtgctcccat gacgatacgc aaaaaattta cgcttacctt
    14761 tcccgctaac aaaaacattc ccccagcatt gggggaatca ccaccaacct gtcggcaacg
    14821 cgtttctccg actttgctca aaagtcatgt gataacaaag gggtgaacta tggccagtgg
    14881 cgatcttgtc cgttatgtca taaccgtcat gttgcatgag gatacattga ctgaaattaa
    14941 cgagttgaat aattacctga ctcgcgacgg ttttttgctc accatgacgg atgatgaggg
    15001 aaatatccat gagctgggga ctaacacttt tggacttatc agtacccaaa gtgaagaaga
    15061 aattagagaa ctggtttcgg ggcttaccca aagtgcaacc ggcaaagatc ctgaaatcac
    15121 catcacgact tgggaggaat ggaatagcaa cagaaaataa atggtttttg ggcaataatc
    15181 agtctgtggt gtgcgttagc tcgtgttttt acaccgcatt cttgcgctaa ccttatgatc
    15241 tggcagacaa catgggagag acatcatgtg gcaggcaatc agtcgtcttt tgagcgagca
    15301 gttaggtgaa ggcgaaatcg aactgcgtaa tgaactgcct ggcggagaag tccatgccgc
    15361 atggcatttg cgctatgcag gacatgactt tttcgtcaaa tgtgatgaaa gggaactgct
    15421 tcccggtttt accgccgaag ccgaccaact ggagttactg tcgcgtagta aaaccgtcac
    15481 cgtgcctaag gtttgggcag taggcgctga ccgtgactac agttttctgg tgatggatta
    15541 tctcccacct cgtccgctgg atgcgcatag cgcatttatt cttggtcagc aaattgcgcg
    15601 tttacatcaa tggagtgacc aaccacaatt tggcctcgat tttgataact cgctctccac
    15661 aactccacag cccaacacct ggcaacgtcg ctggtcaacg ttttttgctg aacaacggat
    15721 tggctggcag ttggaactgg cagcagagaa agggatcgct ttcggcaata tcgacgccat
    15781 cgtcgagcat attcagcagc gtctggcctc gcatcaactg cagccttctc tgttgcacgg
    15841 cgatttatgg tccggcaact gtgcgctggg tccggatggc ccgtacattt tcgaccctgc
    15901 ctgctactgg ggtgaccgag agtgcgacct ggcgatgtta ccgctgcata ctgaacagcc
    15961 gccacaaatc tatgacggct atcagtcagt atccccgcta cctgccgatt tccttgaacg
    16021 tcaaccggtt taccaactct acacgctgtt aaatcgtgca agattatttg gcggtcagca
    16081 tttggttatt gctcagcagt cattggatag attattagca gcatgatatg ggttgaggat
    16141 aatggccgct ccgtgcggcc ttttgattaa ataaacccaa gcagagaaaa gaacacataa
    16201 cctgcagcaa tacaaataac cggcaggata tatagtgaga aaaactgcag gaaaatagta
    16261 tggtgcggaa caacaatacg ggactcaatt tgttcacgcg ttagcccctc ttcccctttg
    16321 gctttttcca gaatgagttg atcttcaaca ccttcccgca agaagcgcgc ctggcgactc
    16381 atccgggcac cggaatcctg caacgccagt ccgacaaaaa tcaggatgaa aatcacccag
    16441 aacataacgt tcacgccacc attaaaatta ggggtcgggg agttatacca gaacagattc
    16501 aaaaaaggcg tattcgcctg catcatatcg atcatgacat gagtaaagtc gagcatgacc
    16561 gcattaatgc cttcctggtt ttcctgccgg gtattcataa atttcagcaa agaaatcaat
    16621 gtggagatca atgcaggtat aaaaatcacc caacccaaaa tccttttcaa aacagcaatg
    16681 cgtccagctt gttgatacgt catgagttct ccttgattaa gacgcgtcgt ttcacttagt
    16741 ttacctgtag atatctgttt tcgcccattc tttaaaggcg atatgatagg cgcttaatca
    16801 taagcacggc ttaatacctt acacataatg ctctaaagga gaggttgtaa tgtcaacccc
    16861 gcgtcagatt cttgctgcaa tttttgatat ggatggatta cttatcgact cagaaccttt
    16921 atgggatcga gccgaactgg atgtgatggc aagcctgggg gtggatatct cccgtcgtaa
    16981 cgagctgccg gacaccttag gtttacgcat cgatatggtg gtcgatcttt ggtacgcccg
    17041 gcaaccgtgg aatgggccaa gccgtcagga agtagtagaa cgggttattg cccgtgccat
    17101 ttcactggtt gaagagacac gtccattatt accaggcgtg cgcgaagctg ttgcgttatg
    17161 caaagaacaa ggtttattgg tgggactggc ctccgcgtca ccactacata tgctggaaaa
    17221 agtgttgacc atgtttgact tacgcgacag tttcgatgcc ctcgcctcgg ctgaaaaact
    17281 gccttacagc aagccgcatc cgcaagtata tctcgactgc gcagcaaaac tgggcgttga
    17341 cccactgacc tgcgtggcgc tggaagactc ggtaaatggc atgatcgctt ctaaagcagc
    17401 ccgcatgcgt tccatcgtcg ttcctgcgcc agaagcgcaa aatgatccac gttttgtatt
    17461 agcaaacgtc aaactctcat cgctgacaga actcaccgca aaagaccttc tcggttaatg
    17521 accaggggca gtgatcacgc tgcccctggt tctttatctg aatttgcatt caactgacgg
    17581 attaatcgtc aatttaagag aaagagttac accgtcacca cttccgtgca ctgtataaaa
    17641 atcctatact gtacgtatcg acagtttagt gagttttatc atgacggcgg aaggtcacct
    17701 tctcttttct attgcttgtg cggtatttgc caaaaatgcc gagctgacgc ccgtgctggc
    17761 acagggtgac tggtggcata ttgtcccttc cgcaatcctg acgtgtttgt taccggacat
    17821 cgatcaccca aagtcgtttc ttgggcagcg attaaaatgg atatcaaaac cgatcgcccg
    17881 cgcttttggg catcgtggtt ttacccacag tctgctggcg gtatttgcgc tgctggcaac
    17941 cttttacctt aaggttccgg aaggctggtt cattccggct gatgcgctac aaggaatggt
    18001 gctgggttat ttgagccaca tacttgccga tatgctgaca cccgccggtg ttcccctgct
    18061 ctggccatgc cgctggcgtt tccgcttgcc tatcctggtt ccccaaaagg gcaaccaact
    18121 ggaacgtttt atctgcatgg cattatttgt ctggtcggta tggatgcccc attcattacc
    18181 cgagaacagc gctgttcgtt ggtcatcgca aatgatcaat accttgcaga tccagtttca
    18241 tcggcttatt aagcatcagg ttgaatacta aaaaggcaaa aatcaccttt ctggaataag
    18301 caattccatt tgaatataag agccagctca cagttctgtt aatcttgcgc caacactatg
    18361 actgctacgc agtgatagaa ataataagat caggagaacg gggatgaact ttccattaat
    18421 tgcgaacatc gtggtgttcg ttgtactgct gtttgcgctg gctcagaccc gccataaaca
    18481 gtggagtctg gcgaaaaaag tgctggtggg tctggtgatg ggtgtggttt ttggccttgc
    18541 cctgcatacc atttatggtt ctgacagcca ggtacttaaa gattctgtac agtggtttaa
    18601 catcgttggt aacggctatg ttcaactgct gcaaatgatc gttatgccgt tagtcttcgc
    18661 ctctattctg agcgcggttg cccgtctgca taacgcatct cagttaggca aaatcagttt
    18721 tctgaccatc ggtacgcttt tgtttaccac gctgattgcg gcgctggtcg gtgtgctggt
    18781 caccaacctg tttggtttga cggctgaagg tctggttcag ggtggtgcag aaactgcacg
    18841 tctgaacgcc attgaaagta actatgttgg taaagtctct gatctgagcg ttccgcagct
    18901 ggtcttgtcc tttatcccga aaaacccgtt tgccgatctt accggagcca atccgacgtc
    18961 aattatcagc gtggtaattt ttgccgcatt cctcggcgta gctgcattga aactgctgaa
    19021 ggatgatgcg ccgaaaggtg aacgcgtctt agccgctatc gataccctac aaagctgggt
    19081 gatgaaactg gttcgccttg tcatgcagtt gaccccttac ggcgttctgg ctctaatgac
    19141 caaagtggtt gcaggttcta acttgcaaga catcatcaaa ctgggaagtt tcgttgtcgc
    19201 gtcctacctc ggtctgctga ttatgtttgc agtgcatggc attctgctgg gcattaatgg
    19261 cgtgagtccg ctgaagtact tccgtaaggt atggcctgtg ctgacgtttg cctttaccag
    19321 ccgttccagt gctgcgtcta tcccactgaa tgtggaagca caaacgcgtc gtctgggcgt
    19381 tcctgaatcc atcgccagtt tcgccgcctc tttcggtgca accattggtc agaacggctg
    19441 cgccggtttg tatccggcaa tgctggcggt gatggttgcg cctacggttg gcattaaccc
    19501 gctggacccg atgtggattg cgacgctggt cggtattgtt accgttagtt ccgcaggcgt
    19561 tgccggtgtc ggtggtggtg caactttcgc cgcactgatt gtgctgcctg cgatgggcct
    19621 gccagtaacc ctggtggcgc tgttaatctc cgttgaaccg cttatcgaca tgggccgtac
    19681 ggcgctaaac gttagtggct cgatgacagc tggcacgctg accagccagt ggctgaagca
    19741 aaccgataaa gccattctgg atagcgaaga cgacgccgaa ctggcacacc gttaattctt
    19801 atgctggcaa cggtccgttt tgtatagggg ccgttgcttt actttttaaa tgttccatcg
    19861 cggcgatgca tactgactca tatttaaaca gatcgtcagc gaaaaaaatg tccaacttat
    19921 caatacattc ctggctcaat gaatgtgcct gtagtgagaa gcttaaaacc tcatacccga
    19981 gacgttttct aagtgaacta tctggaatac tcataatatc ttcaagcaca ccacttccca
    20041 ttcccggaag atttagtgga ttaaaaagaa gattgatatt aagcattgat attatcgaag
    20101 gaaactttag gagactctca ttttttttga atcgcagcaa aaatgctccc ggcaggatgt
    20161 atataatttt tttcatagta tatagtcctt aaataatgaa taaccaacct attttttata
    20221 ttgatcaaat tcgtcatttt ttcgggtcgg aattcacagg taaaatccat cccaacaaaa
    20281 ttctcattaa tatctcttat ccgaaactca ctgccataaa gattattaat tatcactcta
    20341 tatttaacat tgtcctgcag atacgtatca aaactaagtg ctggagctcc tggctttgag
    20401 gcatcatagt taatgttaga catataggca atcatacgat tgaagttcga ctctgctatt
    20461 tcatcgcagt cacattgacc cgttgatata aagaaagtga gtgccgccat ggtagactca
    20521 agcctttctt taaaagagtc gaaagccagg cttttaatat ttaaatcacc ataattactc
    20581 tgtattaagt ttgtagaaaa catctcccgc cccatattgt taacaaaatt attatctcat
    20641 ttaaatctaa gtcacttaca atataagttt aagagcgacg ccacaggatg aactatcaaa
    20701 aatagctcat catgattagc aaaacttaac cattttaaaa taaataaaca attaaagaaa
    20761 aaagatcact tatttatagc aatagctcgt caaaggcagc tttttgttac aggtggtttg
    20821 aatgaatgta gcaacgaaat gcagaatttc aggtcatgta actcccggca aaaccgggaa
    20881 gtatgtaatc cttactcagt cacttcccct tcctggcgga tctgatttgc ccaacgttgg
    20941 gcagattcag gcacagtaaa cgccggtgag cgcagaaatg actctcccat cagtacaaac
    21001 gcaacatatt tgccacgcag catccagaca tcacgaaacg aatccatctt tatcgcatgt
    21061 tctggcggcg cgggttccgt gcgtgggaca tagctaataa tctggcggtt ttgctggcgg
    21121 agcggtttct tcattactgg cttcactaaa cgcatattaa aaattagaaa aactgtagtt
    21181 tagccgattt agcccctgta cgtcccgctt tgcgtgtatt tcataacacc gtttccagaa
    21241 tagtctccga agcgggatct ggctggtggt ctatagttag agagtttttt gaccaaaaca
    21301 gcggcccttt cagtaataaa ttaaggagac gagttcaatg tcgcaacata acgaaaagaa
    21361 cctacatcag caccagtcac cactacacga ttccagcgaa gcgaaaccgg ggatggactc
    21421 actggcacct gaggacggat ctcatcgtcc agcggctgaa ccaacaccgc caggtgcaca
    21481 acctaccgcc cctgggagcc tgaaagcccc tgatacacgt aacgaaaaac ttaattctct
    21541 ggaagacgta cgcaaaggca gtgaaaatta tgcgctgacc actaatcagg gcgtgcgcat
    21601 cgccgatgat caaaactcac tgcgtgccgg taaccgtggt ccaacgctgc tggaagattt
    21661 tattctgcgc gagaaaatca cccactttga ccatgagcgc atcccggaac gtattgttca
    21721 tgcacgcgga tcagcagctc acggttattt ccagccatat aaaagcttaa gcgatatcac
    21781 caaagcggat ttcctctcag atccgaacaa aatcacccca gtatttgtta ggttctctac
    21841 cgttcagggt ggcgctggct ctgcagatac cgtgcgtgat atccgtggtt ttgccaccaa
    21901 gttctatact gaagagggta tttttgacct cgttggcaat aacacgccaa tcttctttat
    21961 ccaggatgcg cataaattcc ccgattttgt tcatgcggta aaaccagaac cgcactgggc
    22021 aattccacaa gggcaaagtg cccacgatac tttctgggat tatgtttctc tgcaacctga
    22081 aactctgcac aacgtgatgt gggcgatgtc ggatcgcggc atcccccgca gttaccgcac
    22141 catggaaggc ttcggtattc acaccttccg cctgattaat gccgaaggga aggcaacgtt
    22201 tgtacgtttc cactggaaac cactggcagg taaagcctca ctcgtttggg atgaagcaca
    22261 aaaactcacc ggacgtgacc cggacttcca ccgccgcgag ttgtgggaag ccattgaagc
    22321 aggcgatttt ccggaatacg aactgggctt ccagttgatt cctgaagaag atgaattcaa
    22381 gttcgacttc gatcttctcg atccaaccaa actgatcccg gaagaactgg tgcccgttca
    22441 gcgtgtcggc aaaatggtgc tcaatcgcaa tccggataac ttctttgccg aaaacgaaca
    22501 agcagcattc catccaggtc atattgttcc tggtctggac ttcaccaacg atccgctgtt
    22561 gcagggacgt ttgttctcct ataccgatac acaaatcagt cgtcttggtg ggccgaattt
    22621 ccatgagatt ccgattaacc gtccgacctg cccttaccac aacttccagc gtgatggcat
    22681 gcatcgtatg gggatcgaca ctaacccggc gaattacgaa ccgaactcga tcaacgataa
    22741 ctggccgcgc gaaacaccgc cgggaccgaa acgcggcggt tttgaatcat atcaggaacg
    22801 cgtggaagga aataaagttc gtgagcgcag tccatcgttt ggcgaatatt attcccatcc
    22861 gcgtctgttc tggctaagtc agacgccatt cgagcagcgc catattgtcg atggtttcag
    22921 ttttgagtta agcaaagtcg ttcgtccgta tattcgtgag cgcgttgttg accagctggc
    22981 gcatattgat ctcactctgg cccaggcggt ggcgaaaaat ctcggtatcg aactgactga
    23041 cgaccagctg aatatcaccc cacctccgga cgtcaacggt ctgaaaaagg atccatcctt
    23101 aagtttgtac gccattcctg acggtgatgt gaaaggtcgc gtggtagcga ttttacttaa
    23161 tgatgaagtg agatcggcag accttctggc cattctcaag gccctgaagg ccaaaggcgt
    23221 tcatgccaaa ctgctctact cccgaatggg tgaagtgact gcggatgacg gtacggtgtt
    23281 gcctatagcc gctacctttg ccggtgcacc ttcgctgacg gtcgatgcgg tcattgtccc
    23341 ttgcggcaat atcgcggata tcgctgacaa cggcgatgcc aactactacc tgatggaagc
    23401 ctacaaacac cttaaaccga ttgcgctggc gggagacgcg cgcaagttta aagcaacaat
    23461 caaggtcgct gaccagggtg aagaagggat tgtggaagct gacagcgctg acggtagttt
    23521 tatggatgaa ctgctaacgc tgatggcagc acaccgcgtg tggtcacgca ttcctaagat
    23581 tgacaaaatt cctgcctgat gggaggcggc gcaattgcgc cgcctcaaat gatttacata
    23641 gtgcgctttg tttatgccgg atgcggcgtg aacgccttat ccggcctaca aaactgtgca
    23701 aattcaatat attgcaggaa acacgtaggc ctgataagcg aagcgcatca ggcagttttg
    23761 cgtttgtcag cagtctcaag cggcgcagtt acgccgcctt tgtaggaatt aatcgccgga
    23821 tgcaaggttc acgccgcatc tggcaaacat cctcacttac acatcccgat aactccccaa
    23881 ccgataacca cgctgagcga tagcaccttt caacgacgct gatgtcaaca catccagctc
    23941 cgttaagcgt gggaaacagt aagcactctg acggatagta ttatcgataa acgccggatg
    24001 acacatcacc tccagcgaac gatcaccccg atggcttgcg tcatccagca cttgcaggaa
    24061 cagtgcctcg ctaatctctt caccataaaa cgcgctactg aatccctgag aactgcgcag
    24121 attagccggt aaatcaccag agttagatag cggctgacgg tcgatgcgca acgcaatccc
    24181 ctcttcagcc gcaaacctgg caacaatcgg gaaaatctgc gggaacatat gcacatgatg
    24241 atggctatca agatgcgtag gtttgcgtcc aaatagctca atgaaacgca aatactgact
    24301 ggcaagctcc tgagtaattt cttccagcgg taaagcatct tcttctgcca actgccagat
    24361 ccattttccc agcacaccat cgcgggttaa ccccggcata gctgtcagtg gcttgcccat
    24421 agtaaggaca aagtacatcc ctatggccag actcggttca tcacgactca actgcaccgc
    24481 atggtcaata gcctgcccat tcaccagcgc cgtcgtcgat gtgacaatcc cattgcgaca
    24541 ggcctcgata atgccgtagt tctgaccttt gcttaagcca aaatcatcgg cattaacaat
    24601 cagtaagcgt tccataatca gcctcggtta atgtgctttt ttaagctctg cgatgcagtc
    24661 ggcaaaattt ggcagccatt tctcgtgcgc cagaatcatc tcgcgtgcca gcagctcagc
    24721 atcgcgatcg gaatgcacca acggactaag gtttagcgcc agtaacacat cgttaaattc
    24781 tccgctaagt gcggcgttgc tggcagcaat ctcgaagcct ttaatggtgt gaatcagccc
    24841 catcacttta tcatcgaaat gcgtaatgcg cggatgtggc gtcgcgccat cgcgccccag
    24901 cttacaggtc atttctaccg cccagtctgc cggaatatta tcaatatgcc catgatgcgg
    24961 gatattaacg taatgttcag cttgcttgtc gttgtagata gcgttgatca cttcgcacgc
    25021 tgcatcagag taataagccc caccgcgctg ttccagttct ttcggcttaa ctttcaactc
    25081 cgggttttta tacagctcaa aaagttgttt ctcgactttc tgtactacct gcgctcgtgc
    25141 gccgcctttg tagtattcgc ccatttcaat agccagcatc tctttttgct tgaagtaata
    25201 aagcaaataa gaacacggca acagattcag agaacgaatt aagccttcgc taaatggcag
    25261 atcgaaaata tttttaacgc cagatgcttt taactgcccg gatgccacac catcaagcaa
    25321 ttcggcaaag cgcgacttgc catttaccag cacatcctta atgaacacca tatggttgag
    25381 gccgaacaaa tcgatagata aatcatcgct gtctttcagc atcagaacat cgtgaataaa
    25441 catcttcatg ccgatcggaa tattacacac gccgataaag cgtttaaatc cggtatgacg
    25501 ataaacggct tcagtaacca ttcccgccgg gttagtgaag ttaatcaccc atgcattcgg
    25561 acaaagttct tcgacatctt ttacgatgtc aaaaatcacc ggaatggtac gcagaccttt
    25621 aaacagaccg cccgcgccgt tggtttcctg gccaagataa ccatgactta atggaatacg
    25681 ttcatccagt tcgcgcgccg gtaattggcc tacgcgcagt tgggtagtaa cgaaatcagc
    25741 atctttcaat gcttcgcggc gatccagcgt tttataaagc ttcatcggga cgccagcgtt
    25801 atcaatcatc cgttggcaga gttcaaaaat gatatccagt ttctctttac catcttcgac
    25861 atccaccagc cataattcgc tgaccggcaa ttcgtgataa cgcttaataa atccttccag
    25921 taactccggg gtatagctgc tcccgccacc aatagtgacg acttttaatt tctggctcat
    25981 aatttctccc ttcagtacag aatactgata tctggcatat ctgccccccc ggacataaat
    26041 aatccagcaa caggacagat atgtgaattg tcaggtataa cgacttactg attaaattca
    26101 gtcaatttct tacgatagct cttaggcgta aaggacgtta atttcttaaa cgttttaata
    26161 aacaaactcg ggctactata accggcctca aacgcaatat ccgtcacgga atagttggtc
    26221 atttccagtt gttttttggc aaaattaata cggatctcat taataatctg cattggtgtt
    26281 ttgccataat atcgttgagt cgctcgcgtc aaatattcct gtgatttggc tgacaacgtc
    26341 accatattct ccagcgccga ttcactaaac tgctctttat catgcatctt ttctaccgta
    26401 cttttcagcc actgcggtac atcatcaatc acctgttctt cgcggtaatg acgtaaacgg
    26461 ttaatgacat aaaaagtaac catctcaaca aactcttcca gccctgtttc gcggaaattc
    26521 aatgaagaaa tcactgtttc cacataggtg agaaaagcgt tattggtccg gtatacctgc
    26581 gaagcgacaa agcaataagg caacaacggc aggtaatgct gctcaaaaaa gcgtttactg
    26641 atcccaacgt tcaatatacg cgtggcacca aactcataaa aactttgatg gtgcgaacct
    26701 aacggaataa aaacaaaatc gccccgttcc agtaacacgc gcttaccgtt aatctcctgg
    26761 aaataacgcc cggttaatac cagagtaaat tcataatagt cgtgctgatg cagcccgctg
    26821 atactctcag ttttgttata gataaacaca tggaagtttt tgccattaaa caactgctgt
    26881 tctcgggcag tggcaatttc cggcgcgtta atcactggct gcatcatcga ctccttatgc
    26941 cttcagtttt tcatgaagct caattaattc agtaatcagt tcacgcgcaa gcatggacgt
    27001 cattaaatga tcctgagcgt ggaccagcac cagactcact ttcatcttac cttcgcccgc
    27061 atcgccttca atcagtttcg tctgtaccag atgcgcttca ttcaatgcca tccgtgactg
    27121 atccatcatg gcttttgctg cagcaaaatc gccctgcttc gcctgtttca gtgccgcata
    27181 cgccaggctg cgcgcttgtc cggagttgat gataagcccc atcaccactt cttccagctc
    27241 ttcagcttcc gtttgcgtat cgggaatgtt atcgagatcc atcatacatc gttcctctct
    27301 attcttaccg gcacgattac ccgtaccggc atcgattaaa atttcagggc gttagcgata
    27361 tcttcttcgc gctcttcttt atcaatcgcg ttttgtgctt tgttagccac cacaacaaag
    27421 ggcagataaa ttaacgttgc gataccaagg ttgaagagtg caaccagcaa tgcggcgacg
    27481 ctaccgttgg tgttaaagaa ggctcccaga ccggttggca tggtccacgg tgcaatattg
    27541 gtcaccggag gaatgatgcc catgtagtac gctgcgaggg tgattgccgc cagaatcggt
    27601 tgtaccagta caaacgggat aaacatcacc gggttcatga taattggcag accaaacaga
    27661 atcggttcgt taatctggaa gatgccggac ggcagcgcca gttttgccac ctgacgataa
    27721 tcagcacggc gagaggcgat aaagatagcc aggatcaggc ctaaagtcgc accactgccc
    27781 ccaaggaaaa taaaggagtc cagcatcggc ttggcccaga tatggaaggt cttaccggct
    27841 gccagcgccg cttcgacgga accatattgc tgataggtcg cgatattttc cagcgcccac
    27901 ggcgtcataa tgccgttgtc cagtgcggtc agcgccagcg cgccatgaat accgaagaac
    27961 cagagcagtg gaacaaagat cacataggcc cagcccacca cgctacccaa cgatgccagt
    28021 ggggttgaga tggtatccat aatgatctga tggaagttgg tgccccaggt attcaacgcc
    28081 caggcaataa tccccatcac ggaaagaata ataaagccgg gaattaatgc cgagaaggaa
    28141 cgcgatactg aagcaggtac gctatcgggc aatttaatga cccaattgcg gcggacaata
    28201 aaggtaaaca tttctgccac caccaggcca ataataatcc cggagatgat attcgctcca
    28261 cctaaccagt ttgcgccaac cacataggcc tcaccgacac tatatggggt gacggtcata
    28321 aatgctgcaa cggataacaa cccagccgcc agcgcatcga ctttacgctc ttctgccagc
    28381 gccatgccaa taaagaacgg tgccattaaa gacattatcc ctaatgttcc gttatatacg
    28441 ttgccgccaa tacctttcag gccattaagt gtttcaatgg ttgaggcgtc gaggcgaata
    28501 cctaaggaat aaaaaaacgc cccctcccca aagcttagaa aaacgttgtt aattaataca
    28561 aacatggccc ccgcaagggt taacggcatt aagcgaataa agccattttt gattgcatta
    28621 acgtgtggct gctttcctat tttaactgca aaagggagga gtaccttttc aagcgatgca
    28681 ataacattac tcatagaaaa atacccttaa aaaccgcaat ttaaatattg cggtattgat
    28741 ttatgaaata actctttgac gggaaaattt aaaataaatt aatttgctgc ggctttttta
    28801 atcgctgcaa ccgcagcctt aagcacgcct aaaccatcga ctttgccata aagcagcgag
    28861 tcaattactt caaccggttt gttgggtaac aaacgctgga tttcgggcaa catataagca
    28921 atctgcggcc ctaataacac gacatcggca ttctgacctt tttcaccagc cagtgtttcc
    28981 ggaaatgctt caataatgac cggaacttca tatttttctg cctgtgcgcg catttttgat
    29041 accagtaaag aggtagacat gcccgcagaa caaaacagat aaatgtgttt cttttccata
    29101 aaactgccct catcgacgat tatctgtcag ccagacactc cgcaagcctt aacctgcttc
    29161 catgctctgg gtaacttgcg aaaccaacat gacttttttt gtggctgaaa ggagtatacg
    29221 gtatagacct gagcaatagt atttctttga ccttataaat tgtttctcct tgacctgtgg
    29281 ttatgaccct cttcacattt cgggcaaata attcgcgaag ataattaaga actcaggcca
    29341 aaaaaaaccg gcgcaatggc cggtttccgt tgttactcaa gctttcagac gaattgatta
    29401 cttcgcagcc tgtggatcag tgtcgtattc agcacaggtc tgataaccgg agttgatgac
    29461 atgtccggta tcatctaacg cgacaaagta ggtttctgct ttaccatcac gttgacccag
    29521 gatgtaggtc tggcaagtac cacgagcatg gatcatgctc acttcagacg aaggtttacc
    29581 cgcaatttgt gcaacctgcg cccggctcat gccttttttg acgtctttca ccacaggctg
    29641 tacaaactgg tctttagtac gatcataagc cgtacaaccc gccagcatgg ttaataccgc
    29701 cgctgcactc agaattcctg ccatattctt gttcatattc cgtcctcttg tttatcagcg
    29761 tgttagataa gcctggaata cattgggcgc tttttcaagc ccgcgaacga aacggctccg
    29821 ctttcagagg attcctgtat gacgttttaa ccaccattca gcccgctgtc gcttgtcgtt
    29881 tcagtggcaa cgggttagct ttaaggaagt tttgtctttt ctgtctggag gggttcaatg
    29941 acattgcaac aacaaataat aaaggcgctg ggcgcaaaac cgcagattaa tgctgaagag
    30001 gaaattcgtc gtagtatcga ttttctgaaa agctacctgc aaacttatcc gttcattaaa
    30061 tcactggtgc tcgggatcag cggcggtcag gactccacgc ttgccggaaa gctgtgccag
    30121 atggcgatta atgagctgcg cctggaaacc ggcaacgaat cactgcaatt tattgccgta
    30181 cgcctgccct atggtgttca ggccgacgaa caagattgcc aggatgccat tgcctttatt
    30241 caaccggatc gcgtattaac cgttaatatc aagggcgcgg tattggccag cgaacaggca
    30301 ttgcgggaag cagacattga actgagcgat tttgtccgtg gcaatgaaaa agcgcgtgag
    30361 cggatgaaag cacaatatag cattgcgggt atgaccagcg gtgtcgtggt gggcaccgat
    30421 catgcagcag aagccattac cggattcttc actaaatatg gtgacggcgg tacagacatt
    30481 aatccgctgt atcgtctcaa caaacgtcag ggtaaacagt tactggcggc attaggttgc
    30541 ccggaacacc tttataagaa agcgccaacg gccgatctgg aagatgatcg cccttctctg
    30601 ccagatgaag tggcactcgg cgtgacctat gacaatatcg acgactatct ggaagggaaa
    30661 aacgtacctg aacaggtcgc cagaacaata gagaactggt atctgaaaac cgaacataaa
    30721 cgccgtccgc caattaccgt tttcgatgat ttctggaaaa agtaataatt tgcacataat
    30781 ggtttgtgcg aaaaagagta atttgttcac gccggatgcg gcgtgaacgc cttattcgac
    30841 ctacaaaact atgcaaattc aatatattgc aggagtcgaa taggcctgac aggcgtagca
    30901 cgtcagacgg tgtaaccttt gtcatcgacc cgcctctttt ttaatcgctc cccgcctgtt
    30961 acactggata gataaccagc attcggagtc aacagtggta cggcgtttaa cttctccgcg
    31021 gctcgaattt gaagctgcgg caatttatga atatcccgaa catttacgtt cattccttaa
    31081 tgacttaccc acccgaccag gggtgtatct gtttcatggc gaaagtgaca ccatgccgct
    31141 ctatatcggc aaaagcgtta atatccgcag tcgggtgctt tctcatttac gcaccccgga
    31201 tgaagccgcc atgctacggc aatcccggcg aattagctgg atctgtaccg caggcgaaat
    31261 cggcgctctg ctccttgaag cgcgattaat caaagaacaa cagccgctgt ttaataaacg
    31321 gttgcgccgc aatcgccagc tctgtgccct gcaattaaat gaaaagcgcg tcgatgtggt
    31381 gtatgccaaa gaggtggatt tttcacgagc ccccaacctg tttggcctgt ttgccaatag
    31441 gcgcgcagct ttgcaagcat tgcagaccat cgctgatgaa caaaaacttt gttatggcct
    31501 gctgggactg gaaccgttaa gtcgcggtcg tgcatgtttt cgttcagcgc taaaacgttg
    31561 cgccggagca tgctgcggta aagagagcca tgaggaacat gcgctacgct tgcgccagtc
    31621 tctggagcgt ttgcgggtgg tgtgttggcc ttggcaaggt gcggtggcac tgaaagaaca
    31681 gcacccggaa atgactcaat atcatattat tcaaaactgg ctgtggctgg gggcggttaa
    31741 ttcgctggaa gaagcgacaa cgttaattcg gacacccgcc gggtttgatc acgacggtta
    31801 taaaattctt tgtaagccgc tgctttccgg taactatgaa attactgaac ttgatccggc
    31861 gaatgaccag cgagccagtt gatttcactg aacaacagtt taccttgcgg ttgtaaaagc
    31921 cgcagggtgt gcctgccatc gaaccagcac gcgccctggt cggtagtgag caatttatcg
    31981 cccagttgcc acgccccgtt aatcacaaac accaccccgc cgcgtgagcc aaaggtagta
    32041 aacgtacgtt cggcaattct gactttcgcc ttacagacat ccaggcgtgt catgatgttg
    32101 aaatccatcg acatctgccc ttccgtcagt ttcgctttta ccacctggtc cgctgcaaag
    32161 gcaaaaggct gtaacggttt taaggtatgg ttaaagcggt ctgcgctttc aaggagcatc
    32221 tcgccgcctt ccagcaacgt cactatcctt tccatgccgg gaaacaaaga aaactcccca
    32281 ttcgccgcga tggatgcaat gctggcacgc cagtaaaaat cacgttttgc cggtgggaaa
    32341 gtgcaaattt cgcgcgtttc accggcagca tttcgccaca gattcaccga cattttacgc
    32401 atatcaaagt attccatact cgctcccggc ccgttctttt cattgttatc gttcaggtaa
    32461 ccgtggatgg ttatcggcaa ctcgggtgat ttacgttagt ggtgatcagg ctttttttac
    32521 aactgtttga gaagagaaaa gaaaaccgcc gatcctgtcc accgcattac tgcaaggtag
    32581 tggacaagac cggcggtctt aagttttttg gctgaaagat tattcagcag ttgcaggcat
    32641 tttacctttt g
//
LOCUS       DAZPLI010000031        32421 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZPLI010000031 DAZPLI010000000
VERSION     DAZPLI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.031"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..436
                     /locus_tag="ACHUU3_001412"
     CDS             <1..436
                     /locus_tag="ACHUU3_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR2081527.1"
                     /translation="KVALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQF
                     AVQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAK
                     AKLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            519..1421
                     /locus_tag="ACHUU3_001413"
     CDS             519..1421
                     /locus_tag="ACHUU3_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFR2081528.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1476..3638)
                     /gene="uvrD"
                     /locus_tag="ACHUU3_001414"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1476..3638)
                     /gene="uvrD"
                     /locus_tag="ACHUU3_001414"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR2081529.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3722..4438)
                     /gene="yigB"
                     /locus_tag="ACHUU3_001415"
     CDS             complement(3722..4438)
                     /gene="yigB"
                     /locus_tag="ACHUU3_001415"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR2081530.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4438..5334)
                     /gene="xerC"
                     /locus_tag="ACHUU3_001416"
     CDS             complement(4438..5334)
                     /gene="xerC"
                     /locus_tag="ACHUU3_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR2081531.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENV
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5331..6038)
                     /locus_tag="ACHUU3_001417"
     CDS             complement(5331..6038)
                     /locus_tag="ACHUU3_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFR2081532.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6035..6859)
                     /gene="dapF"
                     /locus_tag="ACHUU3_001418"
     CDS             complement(6035..6859)
                     /gene="dapF"
                     /locus_tag="ACHUU3_001418"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR2081533.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6896..7099)
                     /locus_tag="ACHUU3_001419"
     CDS             complement(6896..7099)
                     /locus_tag="ACHUU3_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR2081534.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            7307..7449
                     /locus_tag="ACHUU3_001420"
                     /pseudo
     CDS             7307..7449
                     /locus_tag="ACHUU3_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7611..7931
                     /gene="cyaY"
                     /locus_tag="ACHUU3_001421"
     CDS             7611..7931
                     /gene="cyaY"
                     /locus_tag="ACHUU3_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR2081535.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7971..10517)
                     /gene="cyaA"
                     /locus_tag="ACHUU3_001422"
     CDS             complement(7971..10517)
                     /gene="cyaA"
                     /locus_tag="ACHUU3_001422"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR2081536.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            10904..11845
                     /gene="hemC"
                     /locus_tag="ACHUU3_001423"
     CDS             10904..11845
                     /gene="hemC"
                     /locus_tag="ACHUU3_001423"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR2081537.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            11842..12582
                     /gene="hemD"
                     /locus_tag="ACHUU3_001424"
     CDS             11842..12582
                     /gene="hemD"
                     /locus_tag="ACHUU3_001424"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR2081538.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            12604..13839
                     /gene="hemX"
                     /locus_tag="ACHUU3_001425"
     CDS             12604..13839
                     /gene="hemX"
                     /locus_tag="ACHUU3_001425"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR2081539.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPQGDTPAAAPQGE"
     gene            13842..15038
                     /gene="hemY"
                     /locus_tag="ACHUU3_001426"
     CDS             13842..15038
                     /gene="hemY"
                     /locus_tag="ACHUU3_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR2081540.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            15700..15837
                     /locus_tag="ACHUU3_001427"
                     /pseudo
     CDS             15700..15837
                     /locus_tag="ACHUU3_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(15945..17180)
                     /locus_tag="ACHUU3_001428"
                     /pseudo
     CDS             complement(15945..17180)
                     /locus_tag="ACHUU3_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
     gene            complement(17327..17403)
                     /locus_tag="ACHUU3_001429"
     tRNA            complement(17327..17403)
                     /locus_tag="ACHUU3_001429"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17367..17369),aa:Pro,seq:tgg)
     gene            complement(17446..17532)
                     /locus_tag="ACHUU3_001430"
     tRNA            complement(17446..17532)
                     /locus_tag="ACHUU3_001430"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17496..17498),aa:Leu,seq:cag)
     gene            complement(17553..17628)
                     /locus_tag="ACHUU3_001431"
     tRNA            complement(17553..17628)
                     /locus_tag="ACHUU3_001431"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17593..17595),aa:His,seq:gtg)
     gene            complement(17687..17763)
                     /locus_tag="ACHUU3_001432"
     tRNA            complement(17687..17763)
                     /locus_tag="ACHUU3_001432"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17727..17729),aa:Arg,seq:ccg)
     gene            complement(17866..19251)
                     /locus_tag="ACHUU3_001433"
     CDS             complement(17866..19251)
                     /locus_tag="ACHUU3_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR2081541.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(19442..20182)
                     /gene="wecG"
                     /locus_tag="ACHUU3_001434"
     CDS             complement(19442..20182)
                     /gene="wecG"
                     /locus_tag="ACHUU3_001434"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR2081542.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(20185..21537)
                     /gene="wzyE"
                     /locus_tag="ACHUU3_001435"
     CDS             complement(20185..21537)
                     /gene="wzyE"
                     /locus_tag="ACHUU3_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR2081543.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWMALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLIIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(21534..22613)
                     /gene="rffT"
                     /locus_tag="ACHUU3_001436"
                     /gene_synonym="wecF"
     CDS             complement(21534..22613)
                     /gene="rffT"
                     /locus_tag="ACHUU3_001436"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR2081544.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(22610..23860)
                     /gene="wzxE"
                     /locus_tag="ACHUU3_001437"
     CDS             complement(22610..23860)
                     /gene="wzxE"
                     /locus_tag="ACHUU3_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR2081545.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(23862..24992)
                     /gene="rffA"
                     /locus_tag="ACHUU3_001438"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(23862..24992)
                     /gene="rffA"
                     /locus_tag="ACHUU3_001438"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR2081546.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(24997..25671)
                     /gene="rffC"
                     /locus_tag="ACHUU3_001439"
                     /gene_synonym="wecD"
     CDS             complement(24997..25671)
                     /gene="rffC"
                     /locus_tag="ACHUU3_001439"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR2081547.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(25649..26530)
                     /gene="rfbA"
                     /locus_tag="ACHUU3_001440"
     CDS             complement(25649..26530)
                     /gene="rfbA"
                     /locus_tag="ACHUU3_001440"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR2081548.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(26549..27616)
                     /gene="rffG"
                     /locus_tag="ACHUU3_001441"
     CDS             complement(26549..27616)
                     /gene="rffG"
                     /locus_tag="ACHUU3_001441"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR2081549.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(27616..28878)
                     /gene="wecC"
                     /locus_tag="ACHUU3_001442"
     CDS             complement(27616..28878)
                     /gene="wecC"
                     /locus_tag="ACHUU3_001442"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR2081550.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(28875..30005)
                     /gene="wecB"
                     /locus_tag="ACHUU3_001443"
     CDS             complement(28875..30005)
                     /gene="wecB"
                     /locus_tag="ACHUU3_001443"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR2081551.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(30061..31107)
                     /gene="wzzE"
                     /locus_tag="ACHUU3_001444"
     CDS             complement(30061..31107)
                     /gene="wzzE"
                     /locus_tag="ACHUU3_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR2081552.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(31119..32222)
                     /gene="wecA"
                     /locus_tag="ACHUU3_001445"
     CDS             complement(31119..32222)
                     /gene="wecA"
                     /locus_tag="ACHUU3_001445"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR2081553.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(32234..>32421)
                     /locus_tag="ACHUU3_001446"
     CDS             complement(32234..>32421)
                     /locus_tag="ACHUU3_001446"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2081554.1"
                     /translation="CFYICNLNAALAMLGKFLEFAGVLCNLHIKWLIFAQDWWVRNGL
                     SLLNKGLRGYTSANNFL"
BASE COUNT         8114 a         8934 c         8148 g         7225 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcgacat cgttcgtctg gatgccttca aaaccagtaa
      121 agagattcag tttgcggtgc agtcagggcc aatgttgatg gaaaacagtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt gatggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagaggaaa ggttaagcat tgtacactcg aggaggccag acaaactggg gctggctaca
      481 cagctgaaaa atcatcagat aaatgaaggg aaacaaaaat gaaaaaaacg ctcgcaacgt
      541 tatttttatt aacgtgtctg ggatcatcat ccgcttatgc ggataacgct ttgattttac
      601 aaactgattt tagcctgaaa gatggtgccg tctcggcgat gaaaggggtc gcattcggtg
      661 ttgaccataa tcttaaaata tttgatttaa cacacgaaat tccaccgtat aacatctggg
      721 aaggcgctta ccgcctgtat cagaccgcca gttactggcc gcaaggttcg gtatttgtca
      781 gtgtggtcga tcctggtgtt ggtacagacc gtaaatcggt agtgctaaaa accaaaaacg
      841 gtcaatactt cgtttcacca gataacggta cgctcacttt ggtggcggaa tcgctgggta
      901 ttgagagcgt ccggcaaatc gatgaaaaaa ccaaccgcct gaaaggttcc gaaaagtcat
      961 ataccttcca cggtcgtgac gtgtatgcct acaccggtgc acgcctggct tccggagcga
     1021 tcacgttcga acaggtgggg ccagagcttc ctgcaaaagt tgtcgaactt tcttatcaga
     1081 aagccaaagc aacaaaaggt gaagtgaagg gcaatatccc gattctcgat attcagtacg
     1141 gcaacgtctg gagcaatatc agcgacgagt tgctgaatca ggcaggaatc aaactcaacg
     1201 acacgttgtg tgtaaccatt tccgaaggtt cccagcaaaa atacgcaggg aaaatgccgt
     1261 atgtcgcaag cttcggcgat gtgccggaag gccagccgat ggtgtatcta aacagcttat
     1321 tgaatgtttc cgtggcgctc aatatggata atttcgcgca gaaacatcaa gttgcgtctg
     1381 gtgctgactg gaatattgat gtgaagaagt gcgctaagta aggtgctgat gcaggatgcg
     1441 gtgtggcgca ccgcatcctg caacggagtt gtaaattaca ccgtctccag ccgggcgtaa
     1501 gccgccacca gccatttgat tccctggccc tggaatgcca cctgcaaacg gctatgttca
     1561 ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg cacgcgctgg
     1621 ccgagtttgt agccgctgtc gttctcgacc atcggcgtac ccatccgctg atggctaacc
     1681 gggcggctta ccgtggcgcg caggcgcacc tcttccacac actcttccgg cagctcgccg
     1741 ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt ttccgcgtag
     1801 gtcagcgtca gtttctgcat cgcacgggtt acgccaacgt aggccagacg gcgttcttct
     1861 tccagacgtc cgccttcatc cagcgacatc tggcttggga acatgccctc ttccataccg
     1921 acgataaaca cctgcgggaa ctccagccct ttcgccgagt gtagcgtcat caactgcacc
     1981 gcatcctgcc aagtatccgc ctgcccttca cctgcttcca gtgccgcatg ggagaggaac
     2041 gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg cgttgccgtc
     2101 accagttcct ctaagttttc gatacgcgtc tgaccttttt cgcccttctc ctgctcgtac
     2161 atggtacgca ggccggagtc tttaattacc cggtcagtct gtacatgcag cggcatatcg
     2221 gcagtttctt gcgctaaggc atcgattaat tccataaatc gctgcaaggc gctggcagct
     2281 cgcccggcga gggctttttc ctgcaacagt tcacgacatg cctgccagag tgttaactgg
     2341 cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc ccgcgttggc
     2401 gtattcacca cgcgctcaaa cgccgcgtcg tcgttacggt tggcaatcag acgcagatac
     2461 gagagcgcgt ctttgatttc ctggcgttcg aagaagcgca tcccgccgta aatacggtac
     2521 ggcatactgg cctgcaacag tgcttcttcc agtacacgcg actgggcgtt actgcggtag
     2581 agaatggcgc actcggcaag cgccccgccg ttgtcctgcc aggttttgat gcggttaacc
     2641 acaaaacgcg cttcgtcgag ttcgttaaaa gcgcaataga gggaaatagg ctcaccgtcc
     2701 gcgccatcgg tccacagttt tttacccaga cgcccgttgt tgttttcaat cagggcgttc
     2761 gcggcgctca gaatattgct ggtagagcgg tagttctgct ccagacggat agtttcagca
     2821 ccggggaaat cattgaggaa acgctgaata ttctctacct gcgccccgcg ccagccgtag
     2881 attgactggt cgtcatcgcc aacgatcatc actttgccgg tatcgcccgc cagcaggcgg
     2941 atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat gttggtaaaa
     3001 cgttcgcggt agtgttgcag gatatgcggc ttgttaagcc acaactcgtg agcgcgcagc
     3061 agcagctcgg cgaagtccac caggcccgca cgatcgcacg cttcctgata cgcctgatac
     3121 accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg cggacgcagg
     3181 ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca ctgcttctcg
     3241 tcgaggttca tggctttgat cagacgctta agcaggcgta gctggtcttc actgtcgagg
     3301 atctggaaat cctgcggtag attggcgtcc atatggtgcg cacgcagcaa acggtgcgcc
     3361 agcccgtgga aggtgccgac ccacatgccg ccctggctgg tgcccatcag ttgcccgata
     3421 cgatgacgca tctccgccgc cgctttgttg gtaaacgtca cagccataat cgagtatggc
     3481 gagcagtttt ccacgctcat cagccaggcg atacgatgca ccagtacgcg cgtcttacca
     3541 ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc ttcgcgctgt
     3601 ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc gtaaaaatag
     3661 gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac agatttactg
     3721 attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgcggc agtaaacggc
     3781 tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc tgcattccgc
     3841 tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag atctcgccga
     3901 tcggcacgtt gagtttttcc gcagccaaaa agtacatatc gctgaacggt tttgagcgcc
     3961 cgtgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac agttccggct
     4021 gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt tttaaggtgt
     4081 cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta atcattgctg
     4141 cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc gcttgttcaa
     4201 tcgaacgaaa acgccaacgc gtcacgtcgt gataaatctc gggttccgct tcccgtaccg
     4261 cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga tgataatttt
     4321 gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta tcgtaaaggg
     4381 tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa aaacgcatta
     4441 tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt gttgaaaatc
     4501 aagatgagta tagatttgcg tggtggagag gttggcatga cccagcagct cctgcacacc
     4561 acgaagatcg ccgctcgact ccagcatatg cgtggcgaac gagtgacgta atttatgcgg
     4621 atgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt tctgcacatt
     4681 acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt cgctaccaaa
     4741 caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac caatcggcag
     4801 gcggcgctct ttgctgcctt tccccattac ccacacttca ccagactcca ggtcgaggtg
     4861 tttgatatcc agccccacca gctcggaaag acgcagaccc gcgccgtaca tcacttccag
     4921 cattgcacgg tcgcgtacag cgaggggatc gttgatatca atgtccagca gccgattcat
     4981 atcgtcgacg tcgatatttt tcggcagatg acgcggcgct ttcggtgccg aaacaccttt
     5041 cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc tacgtagcgc
     5101 agaaagccgt aacgcgaggc ttgctgctcc cagcccttta cggcgactgc gtacagcaaa
     5161 attgcgcacc atcgtcacat cacattgctg ccagctttgc aggacgtttt cgctggcaaa
     5221 attgatgatc gcctcaagct gacgctggta gttaagcagg gttatcgggc taagctggcg
     5281 ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg tcatacgcgt
     5341 tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag taactgcgtt
     5401 ccctgccctt gttgatagtg actggcatcg cgactggtaa acagcacgac acccaaatca
     5461 gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc cggtagcacc
     5521 accagcagct ctggtccgtt aagcggccca agatagtgct gttcctgccc caaacgctga
     5581 atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt cgacggcgca
     5641 cctaagcgcc agcgatccgg aaacaggcgc agactcgcac ctgccaggcc gagatcgcgc
     5701 gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt gagactgcgc
     5761 tgcaggtaga gtaggcgata aaacaggcct tcgttggcga tagcctgttc catcaacagc
     5821 gccatgttct cttccagaac atgaatatga ttacgtgcgc gggccatgtg ccactcgacc
     5881 aacgaaacgg tgccgcgtac cggatgcggc acacgtatcg cttctactgc gcgcgcatta
     5941 cggataaaaa actcaggatt tttaatcaga taatcgacaa ccgcccggtc atcaagctcc
     6001 gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat ccgtcgtaga
     6061 catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag gcgatatcaa
     6121 gacgaccgcc ggggagttcc acgcgtactt cttcggccag caaaccttgc tgaatcccta
     6181 ctgcaaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc ccacgctcat
     6241 aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata ttggcgcgct
     6301 ccggaaaacg ctcgtggctt tccagaacag gaccaagcgt ttctaccgcc gcggtatcga
     6361 catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg cataagattg
     6421 tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga aacggcacgg
     6481 cggaaggttc gaagttgggt tcgcccatat ttacgcggac cagatcatca tcggtgacgg
     6541 tcagaaccat ccgcccgttg gcggtgctga cgcggatatc acgcttattg gtcagtcctt
     6601 tcagacgcac aaaacgggca aagcagcgcg caccgttgcc gcactgcgcc acttcactgc
     6661 catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc ggctcaacca
     6721 ccagcagttg gtcaaaccct acccccaggt gccgatcagc caggcgacga atcagctccg
     6781 gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg ccaaggccat
     6841 gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact gacttttagt
     6901 aattcacctg ggatggacca tcgccagtgg cgcgatcgtt tttatccggc accgtggatt
     6961 gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca ggcgggaaat
     7021 agagcggacc tttcagaccg cagcccgtca ggctgaagag agtaagtaat acagtgagtg
     7081 ccttaaacac gtttttcatt atggattgcc tgtaaagttc atcgctttct gctttctgct
     7141 ttctgctttc tgctttctgc tttctgcttt ctgctttctg ctttctatca tcgcaggaga
     7201 agacgtaaaa gcaagcgttt agcgactctt tatgaagtct gaaaagtgag gaaatgctgc
     7261 gagtataacg cttactcgtg gggatttatg ttttgctggt agtttcgtga tcggcacctc
     7321 gcgccaggat ggctctatgc actatcgcaa tgtggtttgt ttgttgtctt ttttagttcc
     7381 gtcagcgtga ggatgctcgc tcggtaaacc tgatcataat gtttaccaga aacagggaaa
     7441 ggcgtggtgt aactgcgccc taatgaagag acaaatcttt cgctacacag agacatcatt
     7501 attccggtga cggatgaatc gtgccggaac gcagccgcag aggtgcaaac tttgaagccc
     7561 tgaccccatg ccgctatact gccgccatca cttaaaaaca ggatacaaca atgaacgaca
     7621 gtgaatttca tcgcctggct gatcaactct ggctgaccat tgaagaacgc ctggacgact
     7681 gggatggcga cagcgatatc gactgcgaaa tcaacggcgg cgtgctgacc attacctttg
     7741 aaaacggcag caaaatcatt atcaaccgcc aggaaccgct gcaccaggta tggctggcaa
     7801 ccaaacaagg cggctaccat tttgatctga aaggcgatga gtggatttgc gatcgcagcg
     7861 gcgaaacctt ctgggatttg ctggaacagg cggcgacgca gcaggcgggt gaaacagtca
     7921 gtttccgcta agattgcatg ccggataagc ctcgctttcc ggcacgttca tcacgaaaaa
     7981 tattgctgta atagcggcgt atcgtgatcc tgattggcag gcggcatgtt accgatagat
     8041 tttgtgcgga acggaatcac ctgttcacga ccatcaacct tcacaatctg atagaactgc
     8101 ggcaggttga agttgatgaa gcttgagccg taggtgaagc ggtcgtgcga tgacgagtag
     8161 aagcgactga cgtcacgcac cagttcctct ttgctgcctt cgcagtggtg atacacctca
     8221 acccggttgc tttcgtcaag aatgtagata ttaaagccat tctcgtcttg cgtttcttcg
     8281 aagaagaact ggatgatccc ttcactagca aagccgtcca ccaccgccgg taatttgacg
     8341 tgattggttt caacctgcac tgacaggccg tgcagtttgt tatgcgaaat agcgccataa
     8401 aactcgatgg cgttctccag cttctgtacc gatacattca ggcgttcgaa gaacaagccc
     8461 caggtttgac cagaaacgcg cagcgccttg aaacgcccgg tttcctggcg ggtgctggaa
     8521 aggcgcagtt caatacactc agaaaccagt tgctgcacgc gagtacggat taacccgcgc
     8581 agatgctggc tataacagaa gacttccacg ctatctggcg gtgcggcgtc ctgatgcatt
     8641 ttgccgagaa tagttttcag ggcttcgatc atcgattgct cgccgttgaa gtgcagcgta
     8701 cgcacttcgt tccacgagtt gcggtacagc aggtcaacgc tacctaccag gcaattttga
     8761 ttctcgccaa agctgaagac atccagctta cggaaatcaa aatgcaccac ctgattgcgg
     8821 aacgccgctg tcgggtcata ttccaggtta acgataatcg ccagatggcg gatctcacac
     8881 gggctgtaga gcgccttcgg tgtcggtgca ggtaagcgca gcgggaaatg gtgcgacacg
     8941 tcggcgacca tctcctgcaa cttaggcaaa tcgacaatgc cgttaccttt aatatacaaa
     9001 cgggtgcgcg aggtcagcag accgttaaac catgcccacg ccaccagttt attcaggtaa
     9061 cggttatatt ccagcggctg atggctgatg atcgactcaa tatttggcgc gcggttatac
     9121 agataccaac ctgaacggtt agcccggccc ggcggcacat aaataaaggt cagattcggt
     9181 tccgagagat cgggtgaaat ctgcgggttt accagcgtca ctttacctgg taatgcttca
     9241 aacgcggcat acagcttacg cgtcagcacg ccgatatcct gcggactggc ggagacgcta
     9301 aggttattgc gacgcgcaaa gcggatcaga ttacggtagc tctgcatcat cgcgtcgagc
     9361 aactcgttgt gcgcctcacg cacctgatca atcttccagt tagcacggtt atcgagcatt
     9421 gccagacgag cttcgtccca accccactcg ctcactaact ggctcaacac tgcgcgacgc
     9481 cagcctacac aggcgcgttc acggctgagt ttttcgcaca cttttaaata gaagcagcga
     9541 cgtactaaat ccagacgggt gaaatcttca atcgccgtca ggtattcagt aacacgctcc
     9601 agcatcatgc agtatggatc gaggccaaac gatacaatct cgccgtcgtg caaacgctgt
     9661 ttgatatctt tcgccagcag acgtgggttc gggtattccc aggaataggc ttccagcagc
     9721 agtgttttca gcaccgcttt gtatggggaa tcgatactct tgtagagctg ccaaaggctg
     9781 gcaccaaagt actcttcagc agaaagcgag cttaagccac cgagatccag ccattcattt
     9841 ggcgtcagca cgccctgcgc gtaaagcgtc atcacatagt cgtcgtaatg ctcttcttcg
     9901 tcgcacggca ccatattcca cagaatacgc ttaccggcga gacgcacggc ggtacgataa
     9961 aattcgtcaa gcagcagtat atgctgggtg gagccacaat cttcgccccc caggctgccg
    10021 ctttcattat gacgaaagcg gttttcatca atcaggaaga agctgacttc cacacccagc
    10081 gaggcggccc agctttccag caggctgcat ttacgttgta gtaactggcg ctcttcgcta
    10141 tcgagccagg attgatgaca gacccagata tccaggtcag aggaacaact ttgccctacg
    10201 gacgaggtgc tgcccatggt gtatacacca gtaattggaa gctcaccttt cggtggatcc
    10261 tgtactgaca ttccacgata cagttcaagc tcgttcaggt agtggcgttg agtttcatca
    10321 ggcgtgtaaa ggcaaatgcc tttgggaacg ttaccatcaa ggtaacccgg cattagcgga
    10381 tggtgatagt gcaacaatgt cggcagtaga ctgtagacct gttggaatgc aggccccata
    10441 gcagcaagcg cgcgatccac acgcaattga tttatggcat ccagtctctg tttcagagtc
    10501 tcaatataga ggtacaagac gtatcgcctg atttgctacc cgtcatgact gtgattccgc
    10561 caacatcaac ggtaacacgc ggcattcggg atatttcgta tgtcaaaggt aaccgttacc
    10621 acttttcgcg cctggttttt ttagtttcac gacgaaaaaa tggtctaaaa cgtgatcaat
    10681 ttaacacctt gctgattgac cgtaaagaaa gatgcgctac atacaagtgt agcaccgttt
    10741 attctctgta aattccttat tacgacagcg tgaaacgtct gtcaggatcc actgccagac
    10801 ctcattttac ggtttgcgca ggcgtctacg tttcaccaca acactgacat cactctggca
    10861 aggatgttag gatggaccac ggatgataat gacggtaaca agcatgttag acaatgtttt
    10921 aagaattgcc acacgccaaa gcccacttgc actctggcag gcacactatg tcaaagacaa
    10981 gttgatggcg agccatccgg gcctggtcgt tgaactggta ccgatggtga cgcgcggcga
    11041 tgtgattctt gatacgccgc tggcgaaagt aggcggaaaa ggcttatttg taaaagagct
    11101 ggaagtcgcg ctcctcgaaa atcgcgccga tatcgccgta cattcaatga aagatgtgcc
    11161 ggttgaattc ccgcaaggtc tgggactggt cactatttgt gagcgtgaag atcctcgcga
    11221 tgcctttgtg tccaataact atgacagtct ggatgcgtta ccagcaggca gtatcgtcgg
    11281 gacgtccagt ttacgtcgcc agtgccaact ggctgaacgc cgcccggatc tgattatccg
    11341 ctccctgcgc ggcaacgtcg gcactcgcct gagcaaacta gataacggcg aatacgatgc
    11401 catcattctt gccgtagccg gactaaaacg tttaggtctg gagtcccgca ttcgcgccgc
    11461 gttaccaccc gagatttctc ttccggcggt aggacaaggt gcagtcggta ttgaatgccg
    11521 ccttgatgat acgcgcactc gcgagctgct tgccgcgctg aatcaccacg aaactgcact
    11581 gcgcgttacc gcagaacgcg ccatgaatac ccgtctcgaa ggcggatgtc aggtgccaat
    11641 tggtagctac gccgagctta ttgatggcga aatctggctg cgtgcgctgg tcggcgcgcc
    11701 ggacggttcg cagattattc gcggtgaacg ccgcggtgcg ccgcaaaatg ccaaacaaat
    11761 ggggatttcg ctggcagaag agctactgaa taacggcgcg cgcgagatcc tcgctgaagt
    11821 ctataacgga gacgccccgg catgagtatc cttgtcaccc gcccgtctcc cgctggagaa
    11881 gagttagtga gccgtctgcg cacactgggg caggtggcct ggcattttcc gctgattgag
    11941 ttttctccgg gtcaacaatt accgcaactt gctgatcaac tggcagcgct gggggagagc
    12001 gatctgttgt ttgccctctc gcaacacgcg gttgcttttg cccaatcaca gctacatcag
    12061 caagatcgta aatggccccg actacctaat tatttcgcca ttggacgcac caccgcactg
    12121 gcactacata ccgtaagcgg acagaagatt ctctacccgc aggatcggga aatcagcgaa
    12181 gtcttgctac aattacctga attacaaaat attgcgggca aacgtgcgct gatattacgt
    12241 ggcaatggcg gtcgtgagct aattggggat accctgacgg cgcgcggtgc tgaggtcact
    12301 ttttgtgaat gttatcaacg atgcgcaatc cattacgatg gtgcagaaga agcgatgcgc
    12361 tggcaatccc gcgaggtgac gacggtcgtt gttaccagcg gtgaaatgtt gcagcaactc
    12421 tggtcgctga tcccacaatg gtatcgtgag cactggttac tacactgtcg actattggtc
    12481 gtcagtgagc gtttggcgaa actcgcccgg gaactgggct ggcaagacat taaggtcgcc
    12541 gataacgctg ccaacgatgc gcttttacgg gcattacaat aactctcata acaggaagcc
    12601 ataatgacgg aacaagaaaa aacctccgcc gtggttgaag agactaggga ggccgtggac
    12661 accacgtcac aacctgtcgc aacagaaaaa aagagtaaga acaataccgc attgattctc
    12721 agcgcggcgg ctatcgctat tgctctggcg gcgggcgtcg gtttgtatgg ctggggtaaa
    12781 caacaggccg tcaatcagac cgccaccagc gatgccctgg ctaaccaact gacggcattg
    12841 caaaaagccc aggagagcca aaaagccgaa ctggaaggca ttattaagca acaggctacg
    12901 cagcttgaac aggcgaatcg tcagcaagaa acgctggcaa aacagctgga tgaagtccag
    12961 caaaaggtcg ccaccatttc cggcagcgat gctaaaacct ggctgctggc ccaggccgat
    13021 tttctggtga aactcgccgg acggaagctg tggagcgatc aggacgtcac gaccgctgca
    13081 gcgttgctga aaagtgcaga cgccagcctg gcggatatga atgacccgag tctgattacc
    13141 gttcgtcggg caattaccga tgatatcgcc agcctttctg cagtatcgca ggtggattat
    13201 gacggcatca tccttaagct taatcagctt tcaaatcagg tagataacct gcgtctggcc
    13261 gataatgaca gcgatggttc gccgatggat tcagacggtg aagagctttc cagttccatc
    13321 agcgaatggc gtatcaatct gcaaaaaagc tggcagaact ttatggacaa cttcattacg
    13381 attcgccgtc gtgatgacac cgccgtaccg ctgttagcgc caaatcagga tatctatctg
    13441 cgcgaaaata ttcgctctcg cctgctggtc gcagcacaag ctgtaccgcg tcatcaggaa
    13501 gagacttatc gccaggcgct ggagaacgtc tccacctggg tacgtgctta ctacgatact
    13561 gatgatgcca ccaccaaagc gttcctcgac gaggtggacc agttaagcca gcaaaatatc
    13621 tcgatggatc ttccggaaac cctgcaaagc caggcgatgc tggaaaaact gatgcagacc
    13681 cgcgtgcgta acctgctggc acaaccggca gcggggacaa cggaagctaa acctgcacct
    13741 gcacctgcac ctgcacctgc acctgcacct gcacctgcac ctgcacctgc acctgcaccg
    13801 caaggtgata ctccggcagc cgcgccgcaa ggagaataac catgctaaaa gtgttattac
    13861 tctttgtttt gctgattgcg gggatagtgg ttggcccgat gattgccggt catcagggtt
    13921 acgtgctgat ccagactgac aactacaata tcgaaaccag cgtcacgggc ctggcgatta
    13981 ttttgattct ggcgatggta gtgctgtttg ccattgagtg gctactgcgg cggatcttcc
    14041 gcacaggcgc acacacccgt gggtggtttg tcggacgtaa gcgtcgccgt gcccgtaagc
    14101 agaccgaaca ggcgctgctg aaactggcgg aaggcgatta tcagcaagtt gaaaagctga
    14161 tggcgaaaaa tgccgatcac gcggaacaac cggtggtgaa ctatctactg gctgccgaag
    14221 ccgcgcaaca acgtggtgat gaagcacgcg ccaaccaaca tctggaacgc gcagcagagc
    14281 tggccggcaa tgacaccatt ccggtagaaa tcacccgtgt acgtctgcaa ctggcccgta
    14341 atgaaaacca tgctgcacgc cacggcgtgg ataagctgct ggaagttacg ccacgccatc
    14401 cggaagtatt acgtctggcg gaacaggcgt atatccgcac aggtgcatgg agttctctgc
    14461 tggatatcat cccatcaatg gcgaaagccc atgttggtga tgaagaacat cgtgcaatgc
    14521 tggaacaaca ggcatggatt ggcctgatgg atcaggcgcg tgccgataac ggtagtgaag
    14581 gtttgcgtaa ctggtggaaa aaccaaagcc ggaaaacgcg tcatcaggta gcgttgcagg
    14641 tggcaatggc ggaacatctt attgaatgtg acgatcatga tactgcccag caaattatca
    14701 tcgatggcct gaaacgccag tacgacgatc gcctactgct gccgattcct cgactgaaaa
    14761 ccaacaatcc ggaacagctg gaaaaagtgc tgcgccagca gatcaaaaac gtcggcgatc
    14821 gcccgctgtt gtggagcaca ctgggccagt cactgatgaa gcacggagaa tggcaggaag
    14881 catcgctcgc cttccgcgca gcgctgaaac aacgtccgga cgcctacgat tacgcatggc
    14941 ttgccgacgc gctggacaga ctgcacaagc cggaagaagc tgcagcgatg cgtcgcgacg
    15001 gtttgatgtt aacgttgcag aacaacccac cacagtagtt ccttctcacc cggaggcaag
    15061 cacctccggg acctttctga tgcataaaaa aacgcctgct cttattacgg agcaggcgtt
    15121 aaaacaggtc tgtatgacaa cagtgggtgc ttcactcaac gttgtgtcca tggtgtctga
    15181 tgaggcataa gcgacatctg tcagtggacg ataagcaccg taaacggctc tgcgtcattc
    15241 cggagtttat gaggcactaa ggcgaacata agagatggaa tgagcatcta cccgtttatt
    15301 atgccacaga gaatcgggaa ataacatccc ttaacacttg ttatgagata attctgtaat
    15361 cctctttgct tcctgagtga atatttaacc tgagcttgat cctacacata cttattatga
    15421 atgataaaat tcattcaatt aataacacat atattaattg ccgttaaaac taaaaaagca
    15481 ttaataatca acgcaatata ataaacctgc cttacatatc aactgcgcca gaggtaggat
    15541 tgaaaacgct ctcctgattt tccaattcat tttctggata aataaataat ttatttttgt
    15601 cactattatt tatgtaatca tcctgtcagg gagagggatc tcaattatca atgcttaatt
    15661 acgtcatcat tttgatgaca tgaaaccgga gcattttgca tggaattttc gttttcaccc
    15721 aaacttcttg ttgttgctgt cgccgccgct cttcctctca tggccagcgc agcagatacc
    15781 ccgtcaactg ccaccgcacg caaaggcttt gccggatacg atcacccaaa ccagtatcca
    15841 cagaacgctg caatttattg aatttgcacg atcatgtagg ccggataagg cgtttacgcc
    15901 gcatccggca atcaaccgca ggcggccaca aaatggccgc ttttttactt attcaccacc
    15961 agcaaatgtg cctgcataat gtcactggcc gggcgaccgt gcgccagcaa atcagccatt
    16021 gctttaagat atggcggtag atggcggaaa taacgctgat acccggcaca caaataattc
    16081 agccccggtt tgccgctggc atcgagcatg aagcggtgtt tcgggcagcc tccccagcac
    16141 gcttttaaca cgttacaact gcgacactgc gccggtaatt gcttaaattt atcttcacca
    16201 aacacctgct gttgcgggga atcgatcatt tctgcaattg tttgctgatg catattcccc
    16261 agccgatatt gcggataaac atagtgatcg caggcgtaaa cgtcgccgtt gtgctcaaca
    16321 atcaccgagc gcccacaggt tggctgatga tggcaaaccg cacccggcgc accgacaaaa
    16381 ttggcaaacg cccattcgat attcatcacg aaaatcttgc cgacgtcgcg tttgatccag
    16441 tggtcgaata tcgccaccag aaattcacca aattcatcgg ggcgcaccga ccattccgtc
    16501 agttccccct gaatatcacc aggcgcatgc aatttcagtc catcgcgggc agctgtttca
    16561 tcagccagac gctcgaccac cggaataaac tggatgaatt cgactcccgc atcgcacaaa
    16621 aaatcataaa cctgcaacgg ttgctgcgcg ctggtgcggt tgacgcagac cagcacgtta
    16681 tagtcgacat gatgtttttg cagcagcgtc agggcacgca tcaccagctt atgcgttggt
    16741 ctgccacctt tggtcacgcg atattgattg tggatctcag ccggaccgtc cagcgataac
    16801 ccaacaagaa aatgattttc tgccagaaat gcacaccatt tatcatcgag cagcacgccg
    16861 ttagtctgga agctgttact tatcttcctg ccagcaccgt atttcgcctg taactttacg
    16921 gcacggcggt aaaaatccag cccgagtaac gttggttcgc cgccctgtca ggtaaaagcc
    16981 acttcgtttt gcggttcgct ggcagcgata tagtgacgga catacgcttc cagcgtgtca
    17041 tcgtccatat gcgtgactgg cttttcgcgg taaagggatt gtttttcgag ataaaaacag
    17101 tagtcacagt tcagattaca atcggaacca ctcggtttcg ccatcacatg aaaagcacgc
    17161 gttggaacct gttgcagcat ggtcactcct tgcctgaagt catccttcaa tacgttaagg
    17221 gcgaaatcat accgtggggc aatgagttat tacgggcgtt tattgcgaag ggagggtgac
    17281 gaaatgcaca gaaaacaaaa aaccccgccg aagcggggtt caaaattggt cggcgagaga
    17341 ggattcgaac ctccgaccca ctggtcccaa accagttgcg ctaccaagct gcgctactcg
    17401 ccgaaatact gctttttgaa tttttagttc aattctttaa agtcgtggtg cgaggggggg
    17461 gacttgaacc cccacgtccg taaggacact aacacctgaa gctagcgcgt ctaccaattc
    17521 cgccaccttc gcattgtcac aacttctaat aatggggtgg ctaatgggat tcgaacccac
    17581 gacaactgga atcacaatcc agggctctac caactgagct atagccacca ctacaaatct
    17641 tgttacgcgg tattactacc accgcagctc aagcgccggg actaaatggc gcgcccgaca
    17701 ggattcgaac ctgagacctc tgcctccgga gggcagcgct ctatccagct gagctacggg
    17761 cgcttagcgc cgttgcgggg gtggataata cggacttccc acccctctgt ctagtccctt
    17821 tttaaataaa ttgcgcgttt ggttacggtt tgtgcgtttt gctgcttatt cctccagttt
    17881 atgcgctttc ccgtggcgat taaggccaaa aactttataa atcgccgtca ccgccagcat
    17941 aaagatgatg ccaacaaaca gcgacatacg cgtatcttca ttaaagtaca tgccgatcaa
    18001 aacgcaaatc aggaatgcca ttgttacgta gttggcccac gggaacagga ttgagcggaa
    18061 cggatggctg gcaatcgccg ctttatgtgc acgccgaaaa cgcagctggc ttatcaatat
    18121 cacaaaccat ggcaccatcc ccggaagcac gctggcactg tagacgtaga caaacacacg
    18181 ctgcggattg ggaatgatgt agttcaggca tgagccaatt agcagaatag caatagatac
    18241 tgccacaccc gcaactggta cgccgtgacg ggaaactttc gccatcgccg ccggtaactg
    18301 acggtttttc gccagtgcgt agagcatacg tccgcaactg tacatgccgc tgttacagcc
    18361 agagagcgca gccgtcagca ccacaaagtt gataatgccc gccgctgcgg taataccgat
    18421 tttggcaaaa gtcagtacga acgggctgcc gttgctgcca atttcattcc acgggaagat
    18481 ggtgacgata acgaaaatcg cgcctacgta gaaaatcagg atccgccaca gcactttgcc
    18541 tacagcactg cgcagcgtca cctgcggatt cttcgcttca ccggcagtaa tgccaatcag
    18601 ctccacgccc tggtaggacg ccaccacaat acacagcgcg gtcaggaacc ctttccagcc
    18661 acccgcaaag aaaccgccat gctctgtgag attgctaaaa ccaatcgact gcccgccatt
    18721 gccaaagcca aagaaaatca cgcccaggcc aatgacaatc atcacgataa tcgtggtgac
    18781 tttgatcatc gcgaaccaga actcgatttc gccgtacaac cgcaccgccg ccagattcgc
    18841 caacgccacc agcgccaccg cgatcaatgc aggtatccac tgcgccatct ccgggaacca
    18901 gaactggaca taaacgccaa tggcggtgat ttctgagatc cccaccgcca tccacataaa
    18961 ccagtaagac caggcggtga gataaccaaa aaacgggctc atataacgat gcgcataaac
    19021 ggcgaacgaa ccggtaacag gttcaaggaa caacatttcg cccattgaac gcatgatgaa
    19081 aaagacgaac agcccggcga tgatataggc caacaatacg gatggcccgg cccatttcag
    19141 ggtactggcg gcccccataa acaggccgac gccaatagtg ccccccaggg cgatgagttc
    19201 gatatgtcga gcttccagcc cacgctgtag ctctggtttg ttatctgcca taaatcctcg
    19261 tgttgtgttt gcatgctttc cggtgttacc ggttatcgtt atgggtacat cgagtgttgc
    19321 gaatgttttc gtaattcagg agaaatggca aataaagcat taaaaatttg aatgctttgt
    19381 gtaataaaaa agcagacagg cgacggagtg accactccgt cgctttacaa agagaggaaa
    19441 atcagaggtt gccggtgtag tgccagcgta aataacgcag caaacgaagc tgacgcttaa
    19501 tgcggctcgg ctgcgaaagc aggcggtaga gccactccag tcccagcgtt tgccagattt
    19561 tcggtgcgcg ttttacgtga ccggtgaaaa catcgtaagt cccgccaacg cccatataca
    19621 gcgcatctgg atgcaccaga cggcagtcgc gcatgaagat ctcctgcttt ggcgatccca
    19681 tcgcaacggt gacgatttgc gctccgctgg catgaatgcg ttcaaacagc gcctgacgct
    19741 gctcgggttt aaaataacca tcctgactgc caacgatatt cacattccat tggttgcgca
    19801 gtttagcttc agtttgcgcc agcacttcag gtttaccgcc cacaagaaat accggcgtcc
    19861 cttctttgcc tgcgcgcgcc atcagctctt cccagagatc ggcaccggca acgcgggaaa
    19921 cctgcgcctg cgggtacttt ttacgtactg aacgtacaac gctgatgcca tccgcatatt
    19981 taaattcggc agcgttaatt aactccctga cctcggcgtt atcttcaatg gtcagcattt
    20041 tttcagcatt aatggcaacc agcgttccct gcttaagctg cccgtcagca aacagataat
    20101 cgagggcgtg ctgcatatca cgccaaccaa tcaactgtaa gccacgcagc gtataggtcg
    20161 gtgccgtggt gttgttattc attgttatcc ttcaacctgc gtccggagcg atgattttgt
    20221 acgtttatga atgagtccgg cgctttcaaa aagccagtac agcagttttg cgattatcag
    20281 acatgcgccg aagaccacga taaaaaagac tacgcgtgag acaaacgaat ccagcccttc
    20341 acgcgccagc acgatcatat tgaagatcgc cccaaagcag aaactgtgca atatcgcagc
    20401 cttatagcga ttaggctcgc ggttgcccag ctcatacagc cagtcgaacc atttgatgat
    20461 cagaccaacc acgatcgccc cgagcgggat gaacaacgcg ccgcccatca ccaccagtga
    20521 gcctataagc gtaggcgaga tcgccagtcc ggagtggtta ttcagcactt cccaggtaaa
    20581 gtagttggct gagttcagca ccatactcgg gcgacccggc cacagccagg aagggataaa
    20641 gacatagaaa tcgcggacaa ttggagccag gccctggaag tcgatgttgt cgtagttctg
    20701 caacagcaac gccagattct cccacgggga gaaggtgtcg cgagtgagat agagaaacgt
    20761 atagaacgct tcatcgccgc tcacattcat tccatagcgt tttagcgcca tccagaacat
    20821 gccaacaatc cccagcacgc ccgccgccgc cagcatccac aacgaaatcc agccgcgaat
    20881 aatgccaata aacaggaaga tagcgaatgc gatgatgata ttggcgcgag tgccgccgac
    20941 aatcatataa gtcagcaagc caaaggcgac cgtgctgacg aggaaaaaca gccacgcttt
    21001 gctgtcctgg cgcagaaagt agaccaccag catcgccggg atgaaaaagt aaaagaagcg
    21061 ttttaacgcc acgccggaga cttcactgga gaagatctga ctgtaggagt taagccggaa
    21121 cagcaaaaag ccgttgtgca tgaagaagat gccgacgctt accagcgcga tacccatcag
    21181 gatcacccac gtaagattgg tctccacgcg gttcatggta aacagcggac ggcgcggtac
    21241 atcagcaacg cgtttgcgta ggcgggtttt gtaggtgaca tagtaaaccg cgtagaagca
    21301 gcccgcagaa agcaacgcct gcaacaagat ttctggcggc gcgacaccaa catcaaagcg
    21361 aaataccagc acgctggtca gcgggaagcc gaagaaaaag gtgagcaaaa acagcaatga
    21421 aaagaagaca ttgaagttaa agcgcacacg gcgaaactca aaccaggtca gcgtggcaat
    21481 aaacagcgtg cagagcagcc agaccacaaa caggccactg aattgcagca gactcatgcg
    21541 acctccccgg cggcaatcgc caacgcccgc tgccagcctt gtagatagtt agggctaaag
    21601 aaggcaatgg tgtttttatc caccgacgcc aactggcgct gcgcttcacg cacaatatcc
    21661 tcgttgagat cgtcggtagt aaacagcacc ggcaaatgct gttccgtcat atcctgccag
    21721 aacggatttt cccggttaag cacacaagga atgcccgcct gaatcagtaa gcaaagcgta
    21781 ccaatgccct gctggcgggc aaaaataaag taaccaagat cgcactgacg aagtagcgcc
    21841 agataggcgt caaattccag tttttcgctc aaaatttgca gattttcttc gctgaataac
    21901 tccagccccg cctgacgaac ttcctcaatg tacgcttcgt tattaggcgg atatcccatc
    21961 ggcaccacca cttttaccgt atcgccaaat tgctgatgaa cggcgcgcaa ggcagcaata
    22021 tggtcattgc tgcggtcacc ggagttcccc accagaatgg tcattttccc ttcacgttgc
    22081 cgatcattcg ccatcgtatt gagagaaggg tccatccgcg tcgggaagta cagcagttcg
    22141 ccccgcacct ttggatgcgt tttggcaaaa aagctcaaat caccgcgggt ggcaaataca
    22201 cagccgactc gcttttgcgc caggcgacgt agcgggtaaa aaagcttata tctcaagcca
    22261 ctggaaagct catacaggtc tgccccccag atatgccaga aaaactggct gggcttaatg
    22321 ccaccactca gcagagccag ccacagtgtg ggattgaact gaccgtggaa aaaaaatcgc
    22381 tgctgacggt tagcttttgc tttcgcgatg accgcttccg ccagcgattt tttcccaggg
    22441 aaaaattgca cagaaagcgc cggacagcta tcacttaagc cgtcgtcctt gccaacaacc
    22501 ataaactcgc gcgcgtgctc gctcgtcgcg gccagcgcgt cattgaaaaa ccgcaaaacg
    22561 gttcggttat ggtgagggat atccgatccc agtacgtgaa tcagtacagt catgcccgcc
    22621 tacgccagag taaaaacacg ccacaacaaa gagaaaaata gacgatataa gttgccatat
    22681 atgcctgcgc cgcgcccagt gcaccatgcg cagggattag ccagtgggca aataccatca
    22741 ataaagtgaa ctggctgact tccgccagaa tataaaaccg cagtgacgct ttggcgatca
    22801 ccagataacc aaagacataa gcgcccactt ttaacacatc acccactaac tgccaggcaa
    22861 agagatcgcg catagcggta aatttattcg acaacagcaa ccagatagca aaatcacgca
    22921 gcaaccagac ggtaaaactc gccgccgcca ctgccggtaa gacgaatttc agcgatttaa
    22981 ccacttcccg ggtgatatcg cgcttttccg ttagccgcga caacgtgggc agcaaatata
    23041 cgctgaacga tgccgtaata aattgcaggt aggcatcgga aatactgctc accccttgcc
    23101 agatccccac ttcatcccag ctatactgcg ccgccagcag tttacgcatc atgatgtaag
    23161 caacaggcaa ggtcaccgac gtaatcaacg ccatgagcgt aaatttgctc aactgccctg
    23221 ccagaccgtt atcccagctg ggtttcagat agcttaacgg gatgacacca cgtttgatca
    23281 acatgatggc ggcaggaatc accaccagcg cgggaatcag cgccagaccc agcaacgccc
    23341 cttcataacc gcccaaacgg taactgacgt agtacgcgag aacgccaatc aagctgccga
    23401 caatcagcga taacgcatta cctgcggcat cgcgaaagcc tttcatcagc gccagtaaca
    23461 ggttgcccca ggcgatcccc atttgcacca gcgccactaa acgcaccagc ccctgatagt
    23521 cggtattacc aaacagtccc tggctgattg gcgcagctgc cagcacaaaa accagcgcca
    23581 tcagcgtaga gaaaccaagt accatcgctg atgaagtgcc gaccacgcgg cgcagctgtt
    23641 gcggattatc atggtactgg gcaacgtatt tggttacacc gttaaagatg ccagctccgg
    23701 caagcacgcc gagcacggta atcaactggc ggaaatttgc cgccagccca agccccgccg
    23761 gaccaaatga cacagccagc aacttaccga ccagtaaccc ggcaccaatc ttgaccagtg
    23821 tactggccgc cgtccacaag gacgcttttg ccaacgacat atcaggagaa gtagttcaac
    23881 aaagtcgcaa ttaccgtacg ctgattgacg ggcgacaggt tgtagaacag cggcaggcgc
    23941 agcaggcgct cgctctcttt ggtggtgtag cgatcttcac cgtggaactc accaaagcgt
    24001 tcccccgcag ggcaaccgtg cagcggaatg taatggaaca ccgccatgat ttccgcttct
    24061 ttcagaaagt taatcaacgc gctccggtca tcaatatccc gcagtttaat gtagaacata
    24121 tgcgcgttct gcacgcagcc atcgggaatc gacggcagct cgatacgccc ggctttcgcc
    24181 agaggcgcta acgcatcgta gtagttttgc cacagcgcca gacgttgctg gttgatacga
    24241 tccgctgctt ccagttgcgc ccacaggtac gcagcttgca gatcggacat caaatagctg
    24301 gagccaatat cgcgccaggt atatttatcg acctgaccac ggaagaactg gctgcggtta
    24361 gtgccctttt cacggatgat ctcggctcgt tcgattaacg ctttatcgtt aatcagcgtc
    24421 gcgccgcctt caccacccgc cgtatagttt ttggtttcat ggaagctaaa gcagccaata
    24481 tgaccaatgg ttcccagtgc acgccctttg taagtggaca tcacgccctg agcggcatct
    24541 tctaccacaa acagattatg ctttttcgcc aacgccataa tggtgtccat ttcgcaggcc
    24601 acacccgcgt aatggaccgg cacgataacg cgcgttttgt cggtgatcgc cgcttcaatc
    24661 agcgtttcgt cgatgttcat ggtgtccggg cgaacatcca caaaaacgat ttttgcgcca
    24721 cgcagcacaa aggcattggc ggtggagaca aaggtgtagc tcggcatgat cacttcatcg
    24781 ccaggctgga tatcgagcag cagcgccgcc atctccagcg aagcggtgca ggacggcgtc
    24841 agtaacactt tggcgctgcc aaaacgttgc tccagccact gctggcagcg acgggtaaaa
    24901 ccgccatcgc cacacagttt gccgctaccc attgccgact gcatatagtc gagttcggtt
    24961 cccaccaccg gcggtgcgtt aaatggaatc atgtgatcac ctgtataacc agtacgcggt
    25021 gctttctaca ttcgcaccac tttgtatgta tcgtttaagc gcggcggtgt tgcccatttg
    25081 ggtcgccacc cgcaaagttg ttttaccgcg agcatacgcc cagtttagcg ccgtttgcat
    25141 cagctcagca cctgcaccgc gtccagccag caggccaatt cgcgcatctg tcgcattgag
    25201 ttcccgtaaa gagacataac cgcgaatacc gccggacgcc gcacgtaaaa tcagacattg
    25261 atgatcaaag gtgccgcgca cggcattttc aatccactgt gcataaaagc gtccgctggc
    25321 gtcaggcgca taccacggcg cacgaaaacg gctttgcgca aatgcggcgc tggctaactg
    25381 acgtaatgcg ggaatatcgg tctcttgtgc cactacagca ccactatcac tgacattgtt
    25441 cacgggtagt gccaaatcaa cttcaccttc taccagggag aatcccagct gttgcagggc
    25501 atccagttca cccgtatttg atgccgcaat tttggcctgc acccgtgacc acggcgctaa
    25561 cacgtctggc gtcaggagcg gtgcttcaga cgtaatgcgc acgatggcgc tgttaacacc
    25621 aaagaaggcg ttttcccagg ttagtggctc aatactggcg cggacgggca cgaagtaact
    25681 ccagcagata ttggccgtag ccagttttcg ctaatgaact ggcagcacgc ttcacaccct
    25741 cgtcatcgag ccagccgtta cgccaggcga tctcttccag gcaggcaatc ttaaagccct
    25801 ggcgtttttc tactgtctgc acaaaggtac tggcttcaat caggctgtcg tgagtgccag
    25861 tgtccagcca ggcaaatccg cgcccgagca gttcaacggt caggttgccc gcctcgaggt
    25921 acatctggtt gatggaggta atctccagtt caccacgctc cgacggcttc acctgctttg
    25981 cgtactccac gactttactg tcgtagaaat aaagcccggt caccgcccag tttgacttcg
    26041 gctgttttgg cttttcttcc agcgagatag cgcggaaatt gtcgtcaaac tccaccacgc
    26101 caaagcgttc cgggtccatc acctgatagc caaataccgt cgccccttcc gtgcgcgccg
    26161 caacatgacg cagcttcgga ctgaagccct gaccgaagaa gatgttatcg cccagcacca
    26221 gacaagaagg ttcaccgttg aggaaggttt caccgatgat aaaggcctgc gccagaccgt
    26281 ccgggctggg ctgttcggcg tattccagct ggataccgaa ctcactacca tcgcccagca
    26341 ggcgctggaa ataaccttta tcttccggcg tagtgatgat gagaatttcg cggatatcgg
    26401 ccaacatcag caccgacagc ggatagtaaa tcattggctt atcgtaaatc ggcaacagtt
    26461 gcttcgatac gccgcgcgta atcggatgca atcgggtgcc ggaaccgccc gccaggataa
    26521 tacctttcat gtttgcctcc gctgaaaatt agcctttcag acctaaacgc tcgccctgat
    26581 agctgccgtc ctgcacctgc ttccaccagc tttcattagc cagataccac tgcaccgttt
    26641 tacgcattcc actttcaaag gtttcctgtg gcagccagcc aagttcacgg gcaattttcg
    26701 aagcatcaat ggcataacgc agatcatgcc ccggacggtc agcgacaaag gtgatcaagt
    26761 cacgatagtg caccacgccg tgcggcttgt tcggagccag ttcttccagc agctcgcaaa
    26821 tggtttccac aacatcgaga ttcttacgct cgttgtgacc accaatatta taggtttcac
    26881 cgactttccc ggtggtcgcc acgcaataca gcgcgcgggc gtgatcttcc acatacagcc
    26941 agtcacggat ttgctgcccg ttgccatata ccggcagcgg tttacccgcc agcgcgttga
    27001 ggatcatcag cgggatcagt ttttccggaa agtggtaagg accgtagtta ttcgagcagt
    27061 tggtgataag cgtcggcaga ccgtaggtcc gcagccaggc gcgcaccaga tggtcgctgc
    27121 tggcttttga cgcggaataa gggctgctcg gcgcatacgg cgtggtttcg gtgaagaaat
    27181 catccgtcga gtgcaggtca ccatatactt cgtcggtgga gatatgatga aaacggaacg
    27241 ctgatttttt atcttccgtc agcgtattcc agtaagcccg cgccgcttca agcaatgtat
    27301 aagtcccgac aatgttggtt tcaataaacg ctgccgggcc gtcaatagaa cggtcaacat
    27361 ggctttcggc tgccagatgc atgacacagt ctggctgatg ctcagtgaat acgcgtgcca
    27421 gttctgcccg atcgcagata tcaactttct caaaggcaaa gcgctcgctt tgcgcgaccg
    27481 gtgccagcga catcaggttt ccggcgtagg tcagcttatc gaccactacc accgcgtcgc
    27541 tcgtttcgtt gatgatataa cgcaccagcg ccgagccaat aaacccggca ccacctgtta
    27601 tcagaatttt tctcatcagc gccagactcc tttggcatcg acgacatact gctgatggac
    27661 attgtcgcca ttgataactt taaactgact atgatcgacc agcatcacca gcacgtctgc
    27721 cgttgccagc gcctcgtcaa gctgcgccag agtacaaagc ccggtcagtt ttttcggcaa
    27781 ctggtggatg ttaggctcaa caaccagagt ttcgccgcta tgccactggg cgatcagttc
    27841 agcgatttcc atcgccgggc tttcgcgcag gtcatcaata ttcggtttaa acgccagacc
    27901 aaagcaggcg attttcagtt cactggcgcg tttatcggta gccgccaggc aatcagccac
    27961 cgccgctttc acctgatcga taacccagaa cggtttgtga tcgttcactt cgcgcgcggt
    28021 acggataagc cgcgcctgct gggggttctg tgccacgata aaccacggat caacagcaat
    28081 gcagtgaccg cccacgccag ggccaggctg aagaatatta acgcgagggt gacgattcgc
    28141 caggcgaatc agttcccaga cgttaatccc ctgatcggca caaatcagcg acaattcatt
    28201 agcaaaagcg atattcacat cgcggaagct gttttcggtg agcttacaca tttccgccgt
    28261 ccgcgagtta gtgacgacac actcaccttc gaggaaaatt ttgtacagtt cgctggcgcg
    28321 ggtcgaacaa accggcgtca taccaccaat cacgcgatcg tttttaatca gctcgaccat
    28381 aacctggccc ggtaacacgc gttccgggca gtaagcaatg ttgacgtccg cctgctcgcc
    28441 cacctgctgt gggaaagtga gatccggacg catctctgct aaccattctg ccatcttctc
    28501 ggttgacccc accggcgagg tggattcaag gatcaccagc gcgccttttt tcagcactgg
    28561 cgcaatggag cgagcagccg attcaacgta ggtcatatct ggctcatgat cgcctttaaa
    28621 cggcgtgggt acagcaatca gccaggcatc cgcttcaact ggcgtcgtgc tcgctcgtaa
    28681 aaaaccgcct tctacggcag tttttactac gctcgccaaa tcaggctcga cgatatggat
    28741 ttcgccacga ttgatggtat caaccgcgtg ttggttgata tcgacaccaa tcacctgttt
    28801 ttgccgtgag gcaaacgctg ctgctgtagg cagcccgatg taacccagtc cgataacaga
    28861 aatggtcgca aaactcatag tgatatccga ttatttttta acgcttccag aatgcgagag
    28921 catgcctgac catcaccata cgggttatgg gcgcggctca tagtttgata ttcgttttcg
    28981 tcttttaaaa gacgcgtcac ttcctcgaca attcgctgct tatccgtgcc taccagacgc
    29041 accgtacccg ccgtcaccgc ttccggacgc tcagtggtat cgcgcatcac cagcaccggt
    29101 ttccccagcg aaggcgcttc ttcctgaatg ccgcctgagt cggtcaaaat cagccaggcg
    29161 tggttcatca gccagacaaa cggtaaatac tcctggggat cgatcagaat gacatttttc
    29221 acatgcccca gaatgcgatt gaccggctct ctgacgttcg ggttgagatg caccggatag
    29281 acaatctgga tgtcctggtg cgtggtggca atgtctgcca acgcgtggca gatttcttca
    29341 aagccacgac cgaaactttc acgcctgtga ccggtcacca gaatcatctt tttatcgggg
    29401 tcgataaacg ggtaatttgc cgccagttct gaacgcagcg tatcgctgct catcacctgg
    29461 tcacgcaccc ataacagtgc atcaataact gtattaccag taatgaagat tcggctatcc
    29521 gcaacgtttt cacgcagcaa gttttgccgg gaagtttcgg ttggagagaa gtgatacatc
    29581 gccagatgcc cggtcaatgt acggttagcc tcttccggcc acggcgaata aagatcgccc
    29641 gtgcgcagac cagcctcaac gtgaccaaca ggaatacgct gataaaacgc cgccaggctg
    29701 gttgccagcg tcgtcgttgt gtcgccgtga accagcacga cgtctggttt gaactcggca
    29761 agaataggtt ttagcccttc cagaatccga caggttatct ctgtcaggcc ctgtcctggc
    29821 tgcattatgt tgagatcgta gtcaggtaca atggaaaaga gtttcagcac ctgatcgagc
    29881 atctcccgat gctgcgcagt gacgcaaact ttagcctcaa aaaaaggatc ttttgccaac
    29941 gcatgcacca acggcgccat cttgatggct tccgggcgcg taccaaatac agtcagtact
    30001 ttcacatcga ttctcttcga ataagcggcg agcgcctttg cgctcaccgc agcagtgttg
    30061 ctatttcgag caacggcggg ttaatgcgac accagccccg atcagccccc cgacaatgcc
    30121 ccacataatc atcaggaagg cacgacgtgg gctatcgcgt tttaccggtt cttccggcgt
    30181 acgcaaatag cgataggtct gaaaacgcgg atccagggtt ggaccaacat tcagggtgtt
    30241 taacatggcc cgattctgat catagtcgag atcaaaggcc ggaccgacgg cctgtaaatt
    30301 ttccagtcga gcctggagca ttggacgccc aagcaggaac atttctgaat caggtaattc
    30361 ctcggcaggc acatctgtcg cactgcgcga aatattatgc tgctcagcaa ttttcagcgc
    30421 ctgttcaata ctgttcatcc ggcggtcgta gatggctttc gccacctctt cctgacgctt
    30481 cacctgtgct ttcatctgga tggtacgcgc cgcccatgcg cctttcagct catcattcag
    30541 atggctggct gcacgctggc tggcaaaagc aacatactga cgtaacaggt tattagcgtc
    30601 aggcgcggtt tcagcaataa gcttcacgct gtcattgacc gcgcgggtaa agtctccggg
    30661 gataaactgg atgttgttaa tcatttcatc cagcaacgcc gcatcggctt tgctgttgcc
    30721 caccatccgc tgtttgtaat agtcggtttg cagccagaac tctctgcggg tatcccacga
    30781 ggccagttgc ataacaaact ctttataggc ttcgtccatg accgatggtt ggtcggcaga
    30841 agccatgttt gaacggacat ccaggttacg caaaaattgc tgctgcgagt aatatccccc
    30901 cagcatattc accgttggac gatcggtaat cgccgtcgag ctccactcct gacgagcaaa
    30961 aaaagtatac gccagcgcga ttaacgcaaa tgccagcccc atgccaataa tccatagctt
    31021 cccagcccac aaggtacgaa acaacccacg aatatccagt tcattttcag cgtcttcggc
    31081 cggtttccca ggcattggtt gtgtcatcac atcctcattt atttggttaa attggggctg
    31141 ccaccacgat ttctacgcag tctgcgtttt acgcgcttaa taaagcgagc aactttccag
    31201 gcacgcttaa tgcaatatcc atagaggaag aatgctagca aaaagagcac cagcatgacc
    31261 cactccggga caaaatgaga atattctgcc agcacgccaa tggaagcgag cagtgccgca
    31321 gcaagggtaa tcagcacaaa cgcctgacgg gaagtaaacc cggcacgcat gatcaaatgg
    31381 tgaatatgct gacggtcagg agagaatggg ctcatgcctt tacgcaggcg acggtacata
    31441 atcgccacca tatccattag cggaatggcg attatccaca aagcggtaac cgggctgatg
    31501 ggatgggttt tgccctgggt cgtttcgagc aggatccaga taacggtaaa accaatcagc
    31561 gtactgcccg catcacccat aaagactttg tagcggcgac ccaggatacc aaggttaagc
    31621 atgatgtatg gcaggatggc ggcgatcatc gcaaagcacc agattgcgag gctggtttgc
    31681 ccgtcgaacc acaaaatcat accgattgct gcaaacgaga cgcaggacaa cccgcccagc
    31741 aagccatcaa tgccatcaac catgttgaac gcattaatgg ccgcccagac ggcaaatagc
    31801 gtcaggaagt aaccaaacgg tccgagcacc atctcccagg agccaaagat ataacccagg
    31861 ctactgagat aaagtttgcc gaacaccatc ataacaatgc caacagcggc ctgtatggtg
    31921 gcacggattt ttacgctgat atcaaaacgg tcatccagcg cgccaatgaa aacaagcaca
    31981 ccggcacaag cgagatagag agatgcatgc ggaatatagt aatcgacaat tccgaacgtg
    32041 aagcaaatcc ctgcgtaaac cgaaatcccc ccaacgagag gtatcaatcc ctggtgacgt
    32101 ttgcggaagt ttggtttatc cactaaaccg actttttttg ccaccttacg ggcaaaaaac
    32161 agaaacagtg tcgtgaataa aaaaatactg atgagatcag tactcactgt cagtaaattc
    32221 acaatgcatg ctctcagaga aaattattag cagaagtata accacgaaga cctttattca
    32281 gaagggaaag tccgttccga acccaccagt cctgtgcaaa aattaaccat ttgatatgca
    32341 aattgcataa cacgccagca aattccagga attttcctaa catcgccagc gcggcattaa
    32401 gattacagat ataaaagcaa a
//
LOCUS       DAZPLI010000032        32361 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZPLI010000032 DAZPLI010000000
VERSION     DAZPLI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32361
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.032"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(1..2959)
                     /gene="acrF"
                     /locus_tag="ACHUU3_001447"
                     /pseudo
     CDS             complement(1..2959)
                     /gene="acrF"
                     /locus_tag="ACHUU3_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(2971..4128)
                     /gene="acrE"
                     /locus_tag="ACHUU3_001448"
     CDS             complement(2971..4128)
                     /gene="acrE"
                     /locus_tag="ACHUU3_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFR2081555.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            4527..5189
                     /gene="acrS"
                     /locus_tag="ACHUU3_001449"
     CDS             4527..5189
                     /gene="acrS"
                     /locus_tag="ACHUU3_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFR2081556.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(5192..5371)
                     /locus_tag="ACHUU3_001450"
     CDS             complement(5192..5371)
                     /locus_tag="ACHUU3_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR2081557.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(5455..6339)
                     /gene="yhdJ"
                     /locus_tag="ACHUU3_001451"
     CDS             complement(5455..6339)
                     /gene="yhdJ"
                     /locus_tag="ACHUU3_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR2081558.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(6425..6721)
                     /gene="fis"
                     /locus_tag="ACHUU3_001452"
     CDS             complement(6425..6721)
                     /gene="fis"
                     /locus_tag="ACHUU3_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR2081559.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(6747..7712)
                     /gene="dusB"
                     /locus_tag="ACHUU3_001453"
     CDS             complement(6747..7712)
                     /gene="dusB"
                     /locus_tag="ACHUU3_001453"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR2081560.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(8041..8922)
                     /gene="prmA"
                     /locus_tag="ACHUU3_001454"
     CDS             complement(8041..8922)
                     /gene="prmA"
                     /locus_tag="ACHUU3_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR2081561.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(8934..10385)
                     /gene="panF"
                     /locus_tag="ACHUU3_001455"
     CDS             complement(8934..10385)
                     /gene="panF"
                     /locus_tag="ACHUU3_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR2081562.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(10375..10617)
                     /locus_tag="ACHUU3_001456"
     CDS             complement(10375..10617)
                     /locus_tag="ACHUU3_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR2081563.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(10726..12075)
                     /gene="accC"
                     /locus_tag="ACHUU3_001457"
     CDS             complement(10726..12075)
                     /gene="accC"
                     /locus_tag="ACHUU3_001457"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR2081564.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(12086..12556)
                     /gene="accB"
                     /locus_tag="ACHUU3_001458"
     CDS             complement(12086..12556)
                     /gene="accB"
                     /locus_tag="ACHUU3_001458"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR2081565.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(13534..14508)
                     /locus_tag="ACHUU3_001459"
     CDS             complement(13534..14508)
                     /locus_tag="ACHUU3_001459"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR2081566.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            14660..16600
                     /gene="csrD"
                     /locus_tag="ACHUU3_001460"
     CDS             14660..16600
                     /gene="csrD"
                     /locus_tag="ACHUU3_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR2081567.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            16905..17948
                     /gene="mreB"
                     /locus_tag="ACHUU3_001461"
     CDS             16905..17948
                     /gene="mreB"
                     /locus_tag="ACHUU3_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR2081568.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            18014..19117
                     /gene="mreC"
                     /locus_tag="ACHUU3_001462"
     CDS             18014..19117
                     /gene="mreC"
                     /locus_tag="ACHUU3_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR2081569.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            19117..19605
                     /gene="mreD"
                     /locus_tag="ACHUU3_001463"
     CDS             19117..19605
                     /gene="mreD"
                     /locus_tag="ACHUU3_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR2081570.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            19614..20207
                     /gene="yhdE"
                     /locus_tag="ACHUU3_001464"
     CDS             19614..20207
                     /gene="yhdE"
                     /locus_tag="ACHUU3_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR2081571.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            20197..21666
                     /gene="rng"
                     /locus_tag="ACHUU3_001465"
     CDS             20197..21666
                     /gene="rng"
                     /locus_tag="ACHUU3_001465"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR2081572.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            21734..25534
                     /gene="yhdP"
                     /locus_tag="ACHUU3_001466"
     CDS             21734..25534
                     /gene="yhdP"
                     /locus_tag="ACHUU3_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR2081573.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            25690..27135
                     /gene="tldD"
                     /locus_tag="ACHUU3_001467"
     CDS             25690..27135
                     /gene="tldD"
                     /locus_tag="ACHUU3_001467"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR2081574.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(27263..28192)
                     /gene="aaeR"
                     /locus_tag="ACHUU3_001468"
     CDS             complement(27263..28192)
                     /gene="aaeR"
                     /locus_tag="ACHUU3_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFR2081575.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            28375..28578
                     /gene="aaeX"
                     /locus_tag="ACHUU3_001469"
     CDS             28375..28578
                     /gene="aaeX"
                     /locus_tag="ACHUU3_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR2081576.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            28586..29518
                     /gene="aaeA"
                     /locus_tag="ACHUU3_001470"
     CDS             28586..29518
                     /gene="aaeA"
                     /locus_tag="ACHUU3_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR2081577.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            29524..31491
                     /gene="aaeB"
                     /locus_tag="ACHUU3_001471"
     CDS             29524..31491
                     /gene="aaeB"
                     /locus_tag="ACHUU3_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR2081578.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            31583..31855
                     /locus_tag="ACHUU3_001472"
     CDS             31583..31855
                     /locus_tag="ACHUU3_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR2081579.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(31911..32174)
                     /gene="yhcN"
                     /locus_tag="ACHUU3_001473"
     CDS             complement(31911..32174)
                     /gene="yhcN"
                     /locus_tag="ACHUU3_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR2081580.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         7844 a         8127 c         8475 g         7915 t
ORIGIN      
        1 gtaatacgcc gagaataaag gcgagagagg tcatcaggat aggacgcaga cgcatacgta
       61 ctgccatcag tgtcgcttca acaacacctt tacccttttt ctccatgaga tctttagcga
      121 actcaacaat caaaatagcg tttttggccg acaagccaat tgtcgttagc aagcccacca
      181 taaagtagac gtcatttttt tgattaaaga gtgttgccgc cagtagcacg ccgacaatcc
      241 ctaacggcac gaccaacata accgagacag gaattgacca gctttcatag agtgcagcaa
      301 ggcacaggaa gacaaccaca aaggaaattg ctaccagagc gggagcctgg tttcccgata
      361 agcgttcctg atacgacata cctgtccagt cataaccaat gcccgcaggt aattttgacg
      421 caaggttttc catcaatgcc atggcatcgc cggaactggt tcctggcgcg gcttccccct
      481 gaatctccat tgacggcaga ccgttgtagc gttccagtcg cggagagcca tacacccaat
      541 gtgaagtggt aaaggcagag aatggcacca tttcgccgtt ggcgctgcgg acataaagtt
      601 tatcgacatc ttctggcagc atacggaatt tggcatccgc ctgaacatac acctttttca
      661 cgcgaccacg gtcgatgaag tcgttaacgt aagtcccacc cagcgccgtt gaaatggtct
      721 gattgatgtc agaaagtgaa acacctaatg cctgcgcctt ttcctggtca acttccagtt
      781 taaactgcgc ggtgtcttcc agaccattag gacgcacgct gactaagctg gcaggatgtt
      841 gcgccgccat gccaagcaac tggttacggg cctgggtcag ggcatcgtga cccagcccag
      901 cctgatcaat taactcaaag tcgaaacccg ttgccgtgcc cagttcaaca atggctggca
      961 tattgaatgg aatgacaaaa ccgtcgcgga tcttgcccaa ttccattttg gcacgatgga
     1021 ttaccgcttc cgcactgttt tcgtcaccac tacgctcttc ccacggtttc agactgacga
     1081 aagccatacc ggcgttttgt gcctggccgc tgaagctaaa gccgttaacc gtaaagacac
     1141 tttctacgtt cgctttctcg ttattcagat agtaatccgt cacttgatcc aacacttttt
     1201 gcgtccgctc ttgcgtcgcg ccagcgggta actgaatcat ggtcagaaag acaccctgat
     1261 cctcttcagg taagaaggaa gacggaagac gtaaaaacaa caccaccatt cctgcaacaa
     1321 tcagcgcata gatcagtaaa tatcgtcctg tggaaccgag gattttgccg acgctgttgg
     1381 tgtagtggtt aacgctatga tcgaaggtgg tattaaacca gccgaagaaa ccgcccttat
     1441 tttcgtgatg ctcagcagag acgggtttaa gcagcgttgc acataacgca ggggtaagaa
     1501 tcaatgccac cagaacagaa agcgccattg ccgaaacgat ggtgatagag aactggcgat
     1561 aaattgcccc agtagaaccg ccgaagaatg ccatcggaat aaataccgct gacagcacca
     1621 tcgcgatacc caccagtgcg ccctgaattt gcgacatcga tttttccgtc gcttcttttg
     1681 gcgggagctt atcctccatc atcacgcgct cgacgttctc caccaccact atcgcatcat
     1741 cgacgagcag cccgatggca agcaccatcc cgaacatcgt tagtgtgttg atggagtaac
     1801 caaaagcggc gaggatggca aacgtcccta acaacaccac gggtaccgca atggtgggga
     1861 tcagcgttgc tcgcatattc tgcaagaaca gatacatcac caggaacacc agcataatgg
     1921 cttcgaacag cgtttttacc acttcgtgaa tagaaagctg gacgaatggc gtggtgtcat
     1981 aagggtagag aaccttcatt ccctgcggga agaatggctg taattccgcc agttttgcct
     2041 taatggcttt cgcggtatcg agagcgttcg cgccggttgc tagcttaatc cccaggcccg
     2101 ccgccggttt tccgttgata cgagcgataa cgttatagtt ttcaccgcca agttcaactc
     2161 gtgcgacatc tttcaggcgt accaccgagc cgtcactgtt tacgcgcagg gttactttgc
     2221 cgaattcttc cggatttttt aaccgcgtct gagcaataat cgaggcgttc aactgttgcc
     2281 ctggtaacgc tggcgttccg cccaactgcc cggcagcgat ctgatcgttc tgtaccttca
     2341 actggttaat cacatcaacc ggtgtcagtt tatatttgtt tagcagatcg gcgtccagcc
     2401 agatacgcat cgcatactgt gcgccgaaaa gctgtacgtc accgacgcca ttcagacggc
     2461 taagcgtatc tttaacgtta gaggccacat agtccgagat atcgtcctgt gtggtgtctg
     2521 ggttatcaga gacaaagccc gccaccatca aatagctgct actggacttt tcaacactga
     2581 tcccctgctg ctgaacctcc tgcggcagca acggcgtggc gagctggagt ttgttctgca
     2641 cctgcacttg cgcgatatca ggatcggtcc cggactggaa ggtaagggta attgtcacgc
     2701 taccggcgga atcgctggtg gaggacatat acatcaggtt atcgatacca ttcatattct
     2761 gttcgataac ctgcgtcacc gtatcctgca cggtctgcgc atcagcacct ggatagtttg
     2821 cctaaacaga aaccgcaggc ggtgcaatgg ttggatactg agcgacgggc aattgtagga
     2881 tcgccagtgc gcccgccatc atcagaataa tggccagcac ccatgcaaat atcggtcgtc
     2941 gaataaaaaa gtttgccatg tcagattacc ttacttcgat gcagtatctg cgggggtatc
     3001 ggtagtggct ttaacctgct ctcccggacg cgctttttgc aggccgctga caatgacttg
     3061 atcgccagat ttcagacctt cgctaatcaa ccatttatcg ccaatcgcct gactggcaac
     3121 gacagggcgc gcttccacct gacttttatc gttaacaatc agcacggttg catcaccacg
     3181 cggtgtgcgg ctaacgcctt gttgcgggat aagaatggcg tcaggttgga cgccttcatc
     3241 aatccgtgca cgcacaaaca tacccggcaa aagcgtatgt tgcgggttag ggaagacagc
     3301 acgtagggtt atggagccgg tgcattcatc aacggtcaca tcagagaatt gcagcgtacc
     3361 tttcagggga taggtttgac cgttttccat gaccaactct acgttgctgg tggcgttttc
     3421 cttatgcaaa tttccttgct ctacggattg cttcagcctc ataaaatcgt tgctggattg
     3481 ggtcacatca acgtagatag gatcgagctg ctggacagtc gccagttcag tcgtttgccc
     3541 attagtgaca agagcgcctt cggtcacggt cgatttgccg atacgtccgc taattggcgc
     3601 agtcactttg gtataagcga gattgatgcg agcgctttcg actgtggctt ttgcggcaat
     3661 cacggcggca tcggcctggc gagcatcagc aatggcctgg tcgtactcct gctggctgat
     3721 gtatttcgta cccacgagcg gaacgtaacg ttttaccgtc aaatgcgcga tggcggcggc
     3781 ggcttcactt ttcgccagtt cgcctttcgc gctgtcataa tttgcctgat aggtcgcggg
     3841 atcgatctgg tacagggact ggcctgcttg tacatcgctg ccttcagtga aattacgatt
     3901 cagtacgatc ccgctaacct gtgggcgaac ttcggctata cggtaagcat tggtgcggcc
     3961 tggtaattca gtcttaactt ctaacggggc cgttttcaca atatgaacgg taacctgcgg
     4021 ttcaccgacg tgagctttct cttctccctt atcgttacaa ccgacgatta aagccgcgga
     4081 gatcagaata aaggagggca ggaggaaaaa cctggcatgt ttcgtcatta ctattcctca
     4141 aaaaaacaaa agcgcgttat ttacccagaa aggcaacgcg ttaactcgca gaaagaaaaa
     4201 tacagtttgc tatcctacaa attatcattc gtcgatgtaa ggaatagtta tgaatactgg
     4261 cgtctcaagg cacataaaca caaaaaaaga ttaatattct actgttttat tttgacgcgg
     4321 gttgaaagag gcagaattaa aacctcgtaa attgaaatat atattcatgt ggcgaatgta
     4381 ttttaggtaa ataatatata ttatttattt gacgatttat agcaactcaa tgttaccctc
     4441 tcgtataaat acacattaga tgatagatta accttcgcta ttttctcacc ctgtgtcgaa
     4501 tatatttatt tcctgaataa ttaatcatgg caaaaagaac caaagccgaa gctctgaaga
     4561 cccggcaaga actgattgaa actgccatcg cccagtttgc gcaacatggc gtaagcaaga
     4621 cgacgttcaa cgacattgcc gatgccgcta acgttacgcg tggcgctatc tactggcact
     4681 tcgagaacaa gacccaactg tttaatgaga tgtggttgca acagccttca ttgcgggagt
     4741 taatccagga acacttgacg gctggattag agcatgaccc gtttcaacaa ttgcgtgaaa
     4801 aattgattgt cggcttgcaa tatattgcca aaattccccg ccagcaggcg ttgctgaaaa
     4861 tcttatatca caaatgtgaa tttaatgatg ggatgctggc cgagggagtg atacgcgaaa
     4921 agatgggctt taatccgcag actctccgcg aagtattgca ggcgtgtcag caacaaggtt
     4981 gtgtagcaaa taacctcgat ttagatgttg tgatgattat tattgatggt gccttcagcg
     5041 gaattgttca aaactggtta atgaatatgg cgggttatga tctttataaa caagcacccg
     5101 cgctggtcga taacgtatta agaatgttca tgccagatga aaacataacg aaattaattc
     5161 atcaaacgaa tgaattaagt gtcatgtaaa ttcacattct gtcctgaaaa ttcagttcgt
     5221 cacagttaac cagttttagt ggataaactg agttcatatt acggcatttg tctgtttctg
     5281 ttgtcagcag ttcaatccac tgcatcagta aagtatcaaa cagaaaaacg aagacagcga
     5341 aaacgacgag ccaccaatac ttgcgaatca ttacctgagc ctgtgcatgt ttaaccaagt
     5401 ctggcaggaa atatacacga aatgaataat ctgaaaacca ggcttacact tcctttactt
     5461 tgcaatgaga tcggggtcaa cttcgcttaa ccggcttcgt tttgacaggt tgccggtctt
     5521 tctttttttc actttcgcca gttcttccgc ggagtaatgc gacgcgacat ccagccgtcg
     5581 aagccccatt ttgatgtact cgctgttgat ctcaatacca atgaattttc gtccgctggc
     5641 gatggctacg gcaccggtag taaagctacc ggcaaacggg tcgagaacga tatcgcctgg
     5701 gttggaagag gcgagaataa tgcgtttcag taaggcttcc ggtttttgcg tcgggtggtt
     5761 ctcatattca tccatcaaat aacgcacgcg cggaaaatcc cagacgttac ccggtacttt
     5821 ttgatgattg tatggctgtg gaggattttt gcgataatcg atcaacgcgc gctgcgatcc
     5881 ggttttggct tctaccagaa tagcatcacc gttgaatgtg tagttctttg cgtctttcac
     5941 catcatcagg atgggttcgt acatggagcc gtagtgtttt ttcgcctgta ctccagaact
     6001 gtcatatgac cagacgatgc gacttttgat ggtaaaaagc ttgcggcact ggagatcgat
     6061 aaagggcatg ttttccgtac tgttcataat gtacatgctg ccctgctttt tcagaacgcg
     6121 gtggcactct acaatcactt caaacagcca gtcgataaac agatcttctt tccaggcttc
     6181 gatcagacca tcaaaatttt taccgatgtt atacggtggg tcggcaaaga tcagatcgac
     6241 gctttcagta ggtagctttt taagttcggc aagggcatca ccgtgaataa tggtcttagc
     6301 ttcattacca aaccgggtcg gttcacatcc tgttctcatg gtcactccct ttgtgacacc
     6361 tataaaaaag gcgcttcccc atgccgagta gcgccttttt aatcaagcat ttagctaacc
     6421 tgaattagtt catgccgtat tttttcaatt ttttacgcag cgtaccacgg ttgatgccca
     6481 tcatcagcgc agcacgggtc tggttaccac gggtgtattg catcaccatg tccaacaggg
     6541 gctgttctac ttcagccagt accagctcat agaggtcatt cacatcctga ccattcagtt
     6601 gagcaaaata gttcttcagt gcctgtttaa ccgagtcacg caggggtttt tgggttacct
     6661 gatcctgaga gttaacggta gaaacggtca gtacgtcaga atttacgcgt tgttcgaaca
     6721 tagttctgtc agctctttat ttctgtttac gcaaaatttt cgaagtatgc ctccaacgcc
     6781 tccagctgtt cgctggcatc ctcaatggcg ttgaatgtgc gccgaaactg gtcatttgga
     6841 gcgtgttcct ggagatacca ggaaacgtgt ttacgtgcaa ttcggtaccc ttttgccgga
     6901 ccataaaagt catgcagttc ccgaacgtgc gcgcaaagca agcgcttaac ctctgccaaa
     6961 ggcagcgggg gcagcaactc cccagtgtcc agataatgct ggatttcccg aaagatccag
     7021 ggtcttccct gagctgcgcg gcctatcatc agggcatccg cccctgtata gtcgagcaca
     7081 gctctggctt taagcgggtc agtaatgtcg ccattcgcga taaccggaat ggaaactttc
     7141 tgcttaactg cccgaatact gtcgtactca gcttctccat tgaacaaaca ggcgcgtgta
     7201 cggccatgaa tggtcagagc ctgaatgcca cagtcttcag ccagttgggc aatctcttcg
     7261 cagttacggt gttccggtgc ccagccggtg cgaatcttca gggtaacagg aacgtccact
     7321 gcattgacga cctcggtaag gatcgattta acgacatccg ggtactgcaa gagggctgaa
     7381 cctgcgagct tgcgattcac ttttttagcc gggcaaccca tattgatatc aataatctgg
     7441 gcaccgcttt ccacgttaat acgtgctgca tctgccattt ctttcggatc gctaccagca
     7501 atttgcacgg tgcgaatacc gggttcatca atgtgcacca tccgtaaacg agatttgtcg
     7561 ctttcccaaa cctgtgggtt agaagacatc atctcggata ctgtcaatcc ggctcccatc
     7621 tcgtagcaca acgtccgaaa aggtctgtct gtaatgccag ccatgggcgc tgcgatcagg
     7681 cgatttctga gctgatattg tccgatgcgc atgagttaag aaatgaccat actgtgactg
     7741 caaggcggcg tatattacgc attttttgca cgagatgaaa ggccaaactt tgaccaatcc
     7801 tctgagatgg atcaaagaat tgcatttaaa atgagcgtgg tgagataatt actcataaaa
     7861 atcatcatat tagaaaatag tgactaaaat ttacactcaa agaaatttga gtaagttctc
     7921 aatttttctt tatgaatgaa aattttggca cgcaaatttg cgtaaataac cggcaaattt
     7981 acgtgccttt tgtgagcttg ctcgcacttc gccccgcgtc accctacggc gatgcgaagg
     8041 ttaattctta cgaccggtaa tgcggcacca ctcttctttt tccacgaccg ggtccagtgc
     8101 gaagctgtcg acataagctt cacaaacgct ctctgcctgg cttgccagaa taccggaaag
     8161 gcccagcaaa ccgcctgaaa ccggcaggac gctgattaac ggtgccagtt cacgtaatgg
     8221 gcctgcaagg atgttagcga ccaccacgtc ggctttcatt tcttctggct gatctttcgg
     8281 taagtagagt tccagacggt cagaaacgcc attacgttcg gcgttgtcgc ggctagcctg
     8341 aatcgcctgc ggatcgatat caataccaat ggcttttgct gcacccagtt tcagcgccgc
     8401 gatagccaga atgccggaac cacagccaaa gtcgatgact attttaccgg ttaaatcgag
     8461 gctgtcgagc cactgcaggc acagagaggt agttggatgg gtacccgtac caaacgccag
     8521 ccctggatct aacatcacgt tgacggcgtt ttcgtccggc acatcacgcc agctagggca
     8581 gatccacagt cgttcaccaa agcgcatcgg gtggaaatta tccatccatt cgcgctccca
     8641 gtctttatct tccagttgtt cgattttatg cgcgaagcct gcgccgagca gcggatggtt
     8701 ttccagaatc gccaccacgt cgttcatatc ggtttcagcg tcgaacagac caatcacatc
     8761 ggtgtcgccc cacaggcgcg tttcgcccgg cagcggctca aatactggcg tatcgtgggt
     8821 atcctgaaaa gtgatagaaa cggcacccgc ttccatcagc gcatcgctaa gatcttcagc
     8881 gttcgcgccg gtggtgttca gtttcagttg gatccaaggc atggcaaaac tctttattta
     8941 tcagtagtca aaacggtagc ttgcgggacg gatgtaccga aacggtttcc gaccaggaaa
     9001 gccagcaaac ttagtagtaa cgagggcacg atagggtgga agcccaggta ctgaatattc
     9061 agcgtcgcga gtacggcata cagcacgccg ccaacgatca tcgcacttag cgcgcctttg
     9121 gcgttggcgc gttcccagta aagacccagc accagcggcc acaggaaaac ggcttccagc
     9181 ccaccgaagg ccagcaaatt cagccagatg atcatttctg gcggcttcca ggcggcaagc
     9241 agcagcaacg cgccgagaac taacgtaatt accgccgaca tccgcttcag acgcgtctcg
     9301 ttttgcattt gatccggacg gatattcaga tagagatctt taatgatcgt agcggaactt
     9361 tgtagcaatt gggcgttaat tgtcgacatg atcgcagcca taggtgcagc cagaaagatc
     9421 ccggcagcaa acggtggcag cacttttacc attaacgtcg ggatcaccag atccggtacg
     9481 gtgagatcgg ggatcactgc acgacctaac gctccggcta ggtgcatacc gaacatcagg
     9541 attgcgacta caatcgtacc gatgatgatc ccccggtgta ccgctttgct gtctttataa
     9601 gagatacagc gcaccgcagt atgcggcagg ccaatcacgc caaagcacac cagtacccag
     9661 aacgacgtca taaaggcagg cgacagaata tcgtcagcgc cttgtggcgt aaccagttgc
     9721 ggatcgatgg tttgcaaggt ctgtactgcg ttgcttaagc cgccagcggc atgtaccacg
     9781 ccaataagca gcacaacggt gccaatcagc atcacaagcc cttgcatggt gtcgttcagc
     9841 acgctggcgc gaaagccgcc aaaggcggta tacaacgcaa tgctgatacc gaaaatcagc
     9901 agcccggttt cataaggaat acccgccgcg gtttccagca ggcgcgcacc gccgataaac
     9961 tgcacggtca ttgccccaac gaacgcaacc agcaaactca aactcgccag ccacaccaga
    10021 agacgactct ggtagcgggc aaacagcatg tcgttcagcg tcactgcatt gtagcggcgc
    10081 gcaagaatcg caaacttctt gccgagaata ccgagtgaaa gccagactgc aggaagctga
    10141 atcatcgcca gcaataccca gcccagcccg tatttataag cagctcctgg cccgccgata
    10201 aacgaactgg cactgatata ggtcgcggtg agcgtcatcg ccagcacaat accgcccata
    10261 gagcggctgc cgaggaaata ctcattaagg aaggtgccgg tgctccgttt acgcatcgca
    10321 taaaccgaga taccgaacac caccaccaga taggcgacca gcggtagaat tacttcaagc
    10381 tgcatcgtca tcctccagtg ggatatcgcg atagataaat ttcaccatcg cccagcacag
    10441 tccaataaac agtagcggcg tcaggatgca ggccatctca aaccagcgcg gaaagccggt
    10501 aaaaccgggg gcaacgccag gtaagtaagc ggctactaac caaactgcca gatacaaaag
    10561 ggtcagcccc agcgcccagc gcgcctcttt atgggcctga acaaaacgag tgtccatttt
    10621 ttgtccctga tgggtgaaga aagcgaggat tgtaccttat gggggttgtc gatccccagt
    10681 aataaaaaag gccggaaaat ccggcctttt gacgcttttg cagtcttatt tttcctgaag
    10741 accgagtttt ttctccagat agtggatgtt agtgccacca tgctggaagt tctcgtcatt
    10801 catgatgcgg atctgcagat caacgttggt tttgataccg tcgatgatca gctcctgcag
    10861 cgcattcttc atgcgggcaa tcgccacgtc acggttttca ccgtagcaaa tcagcttacc
    10921 gatcattgag tcatagtacg gcggtacggt gtagcccgcg tagatatgag actcccaacg
    10981 tacgccaaaa ccgccaggtg cgtggaaacg ggtgattttg cctgggcttg gcaggaaggt
    11041 gttcggatct tcggcgttga tacgacattc caccgcatgg ccgcgaacgt gaacttcttc
    11101 ttgcttgatc gacagcggtt gaccggcagc gatacggagc tgttctttga tcaggtcaac
    11161 gccagtgatc atttcggtaa ccgggtgttc tacctgaata cgggtgttca tttcgatgaa
    11221 atagaactcg ccgttttcga acagaaactc gaaagtacct gcaccgcgat agccgatatc
    11281 aacacacgct ttagcgcaac gttcgccgat gtagcgacgc agttccgggg taatgcccgg
    11341 tgctggcgct tcttcgacca ctttctggtg gcggcgctgc atggagcagt cacgttccgc
    11401 cagatagata gcgttgccct gaccgtcagc cagtacctga atctcgacgt ggcgaggatt
    11461 ttccaggtat ttctccatgt aaaccatatc gttgctgaaa gcagctttcg cttccgcacg
    11521 ggtcatggag atggattgtg ccagttcagc gtcgccgcgc actacgcgca taccgcgacc
    11581 accgccgccg ccggaggctt tgataatcac cggataacca atgcgtttag caatggcacg
    11641 gtttttatcc atatcgtcgc ccaacgggcc gtcagaaccc ggtacgcaag ggacgcccgc
    11701 ttttttcatc gccgcgattg cggatacttt gtcgcccatc aggcgaatgg tttctgcttt
    11761 cgggccgatg aagataaagc cggagcgttc aacctgctcg gcaaagttgg cgttctcgga
    11821 gaggaagccg taacccggat ggattgctac tgcgccggtg atttcagcgg cgctgatgat
    11881 tgccgggatg ttcagataac tttttactga cggagcaggg ccaatacaga ccgtttcatc
    11941 tgccagtaat acgtgtttta gatcgcgatc cgcgctggag tgcacagcga cagtcttgat
    12001 gcccagttct ttacaggcac gaagaatacg caatgcaatc tcgccgcggt tggcaataac
    12061 aattttatcc agcatgttcg cctcgttact cgatgacgac cagcggctcg tcaaattcta
    12121 ccggttgtcc actttcgacc agaattgctt tcacggtacc ggatttgtcc gcttcgatct
    12181 ggttcatcat tttcatggct tcaacgatgc acagggtatc gcccacgttg actttctgac
    12241 ccacttcgat gaacgctttt gcgtccgggc ttggggtgcg gtagaaagta ccaaccatcg
    12301 gggaacgtac gatgtgacca ctgatttccg ctgctgctgg cgcttccatg gaaggaacgg
    12361 tcgccggagc ggctgcgtta gattgagctg gctgctgcat cattggtgca gcgtaagctt
    12421 gttgcatcac agggaaactt gcggcaggag ctgcacggct aatgcgtact gactcttcgc
    12481 cttcagaaat ttccagttcg gagatgcctg attcttcaac cagctcgatc agttttttaa
    12541 tcttacgaat atccatgagt gggttccgta ctctttgttt agtgtgattg tgacaggcgt
    12601 ttcaccgccg tctgtaaagc gtatgccccg acagctgtat gcatagcgat aaattccagc
    12661 aggccgggac gccggtctat tttgccccag agtatcaatg ttagacttga cggacattgt
    12721 gcagcgatct gctgctatcc tccggcaaaa aacaaaatat accttcgttg cgccaatgtc
    12781 accttttccg cagcgcaaaa actgcgtcag ggaggacgag gtcgcacatt ataacgattt
    12841 cgtagcaatt ggcagctaaa tactggtctc atcagggaag ataatcaaca gctaacatgt
    12901 aaataacctt caacaccgtg taatttgcaa caagccgcac aattcacgaa attagcgcca
    12961 tcatcgacgg aacttcttat aacgtaaggt gaaaagctgt aaagccagcc ctgcgaagat
    13021 gaggggctgc aatgagataa tcttcacaga ccacaaataa tgtagggatg ccaggatcgc
    13081 gacaagatag acgacattac gcagcggttg ctggggatta cccaattttc actgcatcgt
    13141 ctggatagat gtgaatgcta aagcacgcaa aatcaaccga ctgacaattc tcagcattaa
    13201 ataggttcga gtggttaatt tgtagtcaaa taaaatcaga ttattcaccc catggtacca
    13261 gaaatcagta acaggttaag cgtagtgtcg cccaggcaaa gcaccataaa cctaacaggc
    13321 gacggaggcg tatcaataat ggctgtttag aggagtatca atgcgcatca atttgtggtc
    13381 agtcgaaatc atgactttcc agtttggatg atgttgtaca acttcctgac caaggtgttc
    13441 gacgaaagct gtccggctaa ccgcttttcc cgttaaccga ttgaataatg atgtcaaaat
    13501 gagttccctc tcttattatt ccttgctaaa tggttagtta accttcacca gcgtgcgacc
    13561 ctggatctgg ttattaatga tggcctcggc aaagttcggt gcctctgaca gagatatctc
    13621 ttttgccgcc tgggtataga atgattccgg taaatcggcg accagtcgct gccaggcttg
    13681 tgcgcggcgt tctggtggcg tcattactga atccacccct tgcaaacgga cattacgcag
    13741 aataaatggc atgaccgtgg ttggcagagt aaaaccaccc gccagaccac aggccgccac
    13801 gcagccgccg taattcattt gcgccagcac ttttgccagc actttgtcgc caacagtgtc
    13861 aattgcccca gcccagactt gtttttccag aggacgggat tcggcaaact catcacgagg
    13921 gagaatacgg ctggcaccta aacttttcag atattcatgg gtactttcgc gaccggaaac
    13981 ggcaacgacc tgataaccca acttatgcag cagcgccacg gcggtactgc cgacgccacc
    14041 actggcaccc gtcacgacaa tctccccgtc ctgcgggcga acaccggcat cttccagcgc
    14101 catcacacac agcatggcgg taaaaccggc agtaccgata atcattgctt tacgcgcgtc
    14161 cagcccttgc ggcatggcaa ccagccagtc acctttcact cgcgcctgct ccgccagccc
    14221 accccagtgg ttttcaccaa cgccccagcc agtgagtaac acctcctgac cggcatgaaa
    14281 acgcggatct tcgctggtgc gtacagttcc ggcaaaatcg atcccaggaa tcatcggaaa
    14341 attacggatg atttttccct taccggtaat cgccagcgca tctttatagt tcaggctcga
    14401 ccagtgaaca tcgaccgtga catcgccctc cggcaggcga ctttcgtcca gagtctgtac
    14461 tgatgcgaga gttttgccgt cctgctgttc taaaagtaac gcctgcataa gtggtcctca
    14521 tgtgcatgat ggattggaaa ataatttctg aagattatac tcgctaatgg aaaagcaaag
    14581 ccgatgaagc gcaataaatt gcgagataaa tctgatttgc tagtatgccc gcttcctcac
    14641 tatcggagtt aacacaagga tgagattaac gacgaaattt tcggcctttg ttacgctgct
    14701 caccgggtta acaatttttg tgactttgct gggctgttcg ctaagtttct acaacgcaat
    14761 tcagtataag tttagtcatc gcgttcaggc ggtggcgacg gcgattgata cccaccttgt
    14821 gtcgaatgac ttcagcacat taaggccaca aattaccgaa ttaatgatgt cggcagatat
    14881 cgttcgtgta gacctgctcc atggtgataa acaggtttat accctggcca gaaatggtag
    14941 ttatcgtccg gttggctcca gcgatctgtt tcgcgaactg agtgttccgt tgataaagca
    15001 tccggggatg tcgctgcgtc tggtttatca ggatccgatg ggcaactatt tccattcttt
    15061 aatgactacc gcgccgctca cgggggcgat tggctttatc attgttatgc tcttcctggc
    15121 ggtacgctgg ttacaacggc aacttgccgg gcaagaattg ctggaaaccc gggctactcg
    15181 tatcttaaac ggtgagcgtg gctctaatgt gttgggaacc atctatgaat ggccgcccag
    15241 aaccagcagt gcgctagata cgctgcttcg tgaaattcag aacgcacgcg aacaacacag
    15301 ccgtcttgat acgctgatcc gctcttatgc cgcccaggac gtgaaaaccg gcctcaataa
    15361 ccgactcttt ttcgataatc agttagcaac gttactggaa gatcaggaga aagtaggtac
    15421 ccacgggatc gtgatgatga ttcgtctgcc ggatttcaat atgttgagcg atacttgggg
    15481 gcacagccag gttgaagaac agttcttcac tctgactaat ctgctgtcga catttatgat
    15541 gcgctaccct ggcgcactgc tggcgcgtta ccaccgcagt gattttgctg cgctgttacc
    15601 gcaccggacg ttaaaagagg cagagagcat cgccggtcag ttaatcaaag ccgtcgatac
    15661 cttgccgaac aataaaatgc tcgatcgcga cgatatgatc cacattggta tctgcgcctg
    15721 gcgtagtggt caggataccg agcaggtaat ggaacatgca gagtctgcca cgcgtaatgc
    15781 gggattgcag ggcggcaata gctgggctat ttacgatgac tcgttgcctg aaaaaggacg
    15841 cggtaatgtt cgctggcgta cgcttatcga gcaaatgctc agtcgcggcg gcccgcgcct
    15901 ttatcaaaaa ccggcggtta ctcgcgaagg tcaggttcat catcgcgaac tcatgtgccg
    15961 catcttcgat ggtaatgaag aggttagctc ggcggagtat atgccgatgg tcttgcagtt
    16021 tggcttatcg gaagagtatg accgtctgca aatcagccgt cttattccac tattgcgtta
    16081 ctggccagag gaaaatctgg cgattcaggt taccgttgag tcgctgattc gcccgcgttt
    16141 tcagcgttgg ctgcgcgata cgttaatgca atgtgaaaaa tcacaacgaa aacgcataat
    16201 tattgaactt gcagaggccg atgtaggtca acatatcagt cgtttacaac ctgttattcg
    16261 tttagtgaat gctttagggg tacgggtagc cgtcaaccag gctggtttga cgctggtaag
    16321 caccagttgg atcaaagaac ttaatgttga gttactcaag ctccatccgg ggctggtcag
    16381 aaacattgag aagcgaacgg agaaccagct gctggttcaa agcctggtgg aagcctgctc
    16441 cgggaccagc acccacgttt acgccaccgg cgtgcgttcg cgaagcgagt ggcagaccct
    16501 gattcagcgc ggtgttacag gcgggcaagg ggattttttc gcgtcctcac agccacttga
    16561 tactaacgtg aaaaaatatt cacaaagata ctcggtttaa cctgccgttt aatccgtttt
    16621 cacgtagaat aacgcgcgct gcgtctcatg ggagtgtgct tgtctgctcg ccagattgtt
    16681 gcagcacata tgcagatgaa tgaccttacg cggttgcaaa caggcgagga atgctgctga
    16741 tgcattaagc ctttctggac tcaggcagag atttgtaaca aaggaaacga actgcactaa
    16801 ttttcaccgt agcagatgat ttttgcgcct tgtcgctgct gcgtgtggtt ggtaaagtaa
    16861 gcggattttc ttttccgccc cagctttcag gattatccct tagtatgttg aaaaaatttc
    16921 gtggcatgtt ttccaatgac ttgtccattg acctgggtac tgcgaatacc ctcatttatg
    16981 taaaaggaca aggcatcgta ttgaatgagc cttccgtggt ggccattcgt caggatcgtg
    17041 ccggttcacc gaaaagcgta gctgcagtag gtcatgacgc gaagcagatg ctgggccgta
    17101 cgccgggcaa tattgctgcc attcgcccaa tgaaagacgg cgttatcgcc gacttcttcg
    17161 tgactgaaaa aatgctccag cacttcatca aacaagtgca cagcaacagc tttatgcgtc
    17221 caagcccgcg cgttctggtt tgtgtaccgg ttggcgcgac ccaggttgaa cgccgcgcaa
    17281 ttcgtgaatc cgcgcagggc gctggtgccc gtgaagtctt cctgattgaa gaaccgatgg
    17341 ctgccgcaat tggtgctggc ctgccggttt ctgaagcgac cggttctatg gtggttgata
    17401 tcggtggtgg taccactgaa gttgctgtta tctccttgaa cggcgtggtt tactcctctt
    17461 ccgtgcgcat tggtggtgat cgtttcgacg aagctatcat caactatgtg cgtcgtaatt
    17521 acggttctct gatcggtgaa gccaccgcag aacgtatcaa gcacgaaatc ggttcggctt
    17581 acccgggcga tgaagtccgt gaaatcgaag ttcgtggccg taacctggca gaaggtgttc
    17641 cacgcggttt taccctgaac tccaacgaaa tcctcgaagc actgcaggaa ccgctgaccg
    17701 gtattgtgag cgcggtaatg gttgcactgg aacagtgccc gccggaactg gcttccgaca
    17761 tctccgagcg cggcatggtg ctcaccggtg gtggcgcact gctgcgtaac cttgaccgtt
    17821 tgttaatgga agaaaccggc attccagtag ttgttgctga agacccgctg acctgtgtgg
    17881 cgcgcggtgg cggcaaagcg ctggaaatga tcgacatgca cggcggcgac ctgttcagcg
    17941 aagagtaatc ggatgcaggc aggggaaatg tctgtttacc ctgcctggtc tgatacgaga
    18001 atacgcataa cttatgaagc caatttttag ccgtggcccg tcgctacaga ttcgccttat
    18061 tctggcggtg ctggtggcgc tcggcattat tattgccgac agccgcctgg ggacgttcag
    18121 tcaaatccgt acttatatgg ataccgccgt cagtcctttc tactttgttt ccaatgctcc
    18181 tcgtgaattg ctggatggcg tatcgcagac gctggcctcg cgtgaccaac tagaacttga
    18241 aaaccgggcg ttacgtcagg aactgttgct gaaaaacagt gaactgctga tgcttggaca
    18301 atacaaacag gagaacgcgc gtctgcgcga gctgctgggt tccccgctgc gtcaggatga
    18361 gcagaaaatg gtgactcagg ttatctccac ggttaacgat ccttatagcg atcaagttgt
    18421 tatcgataaa ggtagcgtta atggcgttta tgaaggccag ccggtcatca gcgacaaagg
    18481 tgttgttggt caggtggtgg ccgtcgctaa actgaccagt cgcgtgctgc tgatttgtga
    18541 tgcgacccac gcgctgccaa tccaggtgct gcgcaacgat atccgcgtaa ttgcagccgg
    18601 taacggttgt acggatgatt tgcagcttga gcatctgcca gcgaatacgg atattcgtgt
    18661 tggtgatgtg ctggtgacct ccggtctggg cggtcgtttc ccggaaggct atccggtcgc
    18721 ggttgtctct tccgtaaaac tcgataccca gcgcgcttat actgtgattc aggcgcgtcc
    18781 gactgcagga ctgcaacgtt tgcgttatct gctgctgctg tggggcgcgg atcgtaacgg
    18841 cgctaacccg atgacgccgg aagaggtgca tcgtgttgct aatgaacgtc tgatgcagat
    18901 gatgccgcag gtattgcctt cgccagacgc gatggggcca aagttacctg aaccggcaac
    18961 ggggatcgct cagccgactc cgcagcaacc gacgacagga aatgcagcta ctgcgcctgc
    19021 tgcgcccaca cagcctgctg ctaatcgctc tccacaaagg gctacgccgc cgcaaagtgg
    19081 tgctcaaccg cctgcgcgtg cgccgggagg gcaatagtgg cgagctatcg tagccaggga
    19141 cgttgggtaa tctggctctc tttcctcatt gcgctgttgc tgcaaatcat gccctggccg
    19201 gataacctga ttgttttccg gccaaactgg gtgttactca tcttgttgta ttggatcctg
    19261 gccttgcctc atcgcgtaaa tgtgggcaca ggttttgtga tgggtgccat actggatctg
    19321 atcagcggct cgacgcttgg cgtacgcgta ttggcgatga gcatcattgc ttacctggtg
    19381 gcgctgaaat accagctttt ccgcaacctc gcattatggc agcaggcgct ggtcgtcatg
    19441 ttgctttcgc tggtggtgga tattattgtt ttctgggcag agtttttagt gattaacgtc
    19501 tctttcagac cggaagtgtt ctggagtagt gtagtcaatg gggtgctctg gccgtggatt
    19561 ttcttgctga tgcgtaaagt ccgtcagcag tttgcagtgc aataaaggtt tctatgactt
    19621 ctctgtattt agcttccggt tctccgcgtc gtcaggagtt acttgcgcaa cttggtgtga
    19681 cctttgaacg tattgttacg ggcattgagg agcagcgtca gccgcaggag agcgcgcagc
    19741 agtatgttgt gcgtctggcg cgcgagaaag cacaggcggg tgtcgcgcaa acggcgcagg
    19801 atctcccggt gctgggtgcg gatactatcg ttatcctgaa tggagaagtg ctggagaaac
    19861 cgcgcgacgc agagcatgcg gcgcagatgt tgcgcaaatt atcgggtcag acccatcagg
    19921 tgatgacggc agtggcgttg gccgacagcc agcacattct cgattgcctg gtggtcaccg
    19981 atgtgacttt cagaacgtta acagacgaag acatcgcggg ctatgtcgcc agcggtgaac
    20041 cgttagataa agcaggtgca tacggtattc aggggctggg tggctgtttt gtcaggaaga
    20101 taaatggcag ctatcacgcc gtagtcggct tacctctggt tgaaacgtat gaattattaa
    20161 gtaattttaa cgcactgcgt gagaaaaggg ataaacatga cggctgaatt gttagtaaac
    20221 gtaacgcctt cggaaacgcg agtggcgtat attgatggcg gtattctgca ggaaattcat
    20281 attgaacgtg aggcgcgacg cggaatagta ggcaatatct acaagggtcg tgtaagtcgt
    20341 gtacttccgg gtatgcaggc ggcttttgta gatattgggc tggataaagc cgcgtttctt
    20401 catgcatccg atatcatgcc gcacaccgaa tgtgtggcgg gtgaagaaca aaagcaattc
    20461 acggtgcgcg acatctcgga actggttcgt caggggcaag atctgatggt gcaggtggtg
    20521 aaagatccgc ttggcactaa aggtgcgcgc ctgaccaccg atatcacgct gccttctcgc
    20581 tatctggtct ttatgccagg ggcttctcac gttggggttt cccaacgtat tgaaagcgaa
    20641 tcagaacgtg aacgcctgaa aaaagtggtc gcagagtatt gcgacgagca gggcgggttt
    20701 atcatccgta ccgcagcgga aggggttggc gaggctgaac tggcctccga tgccgcttat
    20761 ctgaaacgcg tctggaccaa agttatggag cgtaaaaagc gcccgcagac ccgttatcag
    20821 ctgtacggcg aactggcgct ggctcagcgt gttctgcgtg atttcgccga cgccgaactg
    20881 gaccgcattc gcgttgactc acgcctgact tacgaagcat tacttgagtt cacctcggag
    20941 tacattcccg agatgaccag caagctggag cattacaccg gacgccagcc gattttcgat
    21001 ctctttgatg tcgaaaacga aatccagcga gcgctggaac gcaaagtaga actgaaatcc
    21061 ggtggttatc tcattatcga ccagaccgaa gcgatgacca ccgtggacat caataccgga
    21121 gcgtttgtcg gtcatcgcaa tctggacgac accattttca ataccaatat tgaagcgacg
    21181 caggctatcg ctcgccagtt acggttgcgt aatctgggcg ggattatcat tattgatttc
    21241 atcgatatga ataatgaaga tcaccgccgc cgcgtgctgc actcgctgga gcaggcgttg
    21301 agcaaagacc gggtgaaaac cagcgttaat ggtttttcgg cgctggggct ggtggagatg
    21361 acgcgtaaac gcacccgcga aagcattgag cacgtactgt gtaacgaatg cccaacctgc
    21421 cacggtcgcg gaacggtgaa aaccgtggaa acggtatgct atgaaatcat gcgcgagatt
    21481 gttcgtgtcc accatgctta cgactccgac cgtttcctgg tctatgcttc tccggcagta
    21541 gccgaagcct tgaaaggcga agagtcacac tcgctggcgg aagtggaaat tttcgttggc
    21601 aaacaggtta aagtacaaat tgaaccgctc tataaccagg agcagtttga cgtcgtaatg
    21661 atgtaaacag atgctgggcc gccatccggc aaagggtttt tgagtcacat ttttagcaga
    21721 caaggagtga cgggtgaggc gattgccggg gattttactg cttactggag ccgcgctcgt
    21781 tgtgatcgct gccctgctgg ttagcggcct gcgtattgct ttaccgcatc ttgacgcctg
    21841 gcgtccggaa atcctcaaca aaatagaatc cgcgactggc atgccggtag aagccagtca
    21901 gctctcagcc agctggcaga attttggccc gacgcttgaa gcacacgaca tccgtgcaga
    21961 actaaaagat ggcggcgaat tttcggttaa acgcgttact ctggcgctgg atgtctggca
    22021 gagcctgtta catatgcgct ggcagtttcg cgacctcact ttctggcagc tgcgctttcg
    22081 caccaacact cctatcacca gcggtggtag cgatgacagt ctggaagcca gtcacatcag
    22141 cgatctgttt cttcgtcaat ttgaccattt cgatctccgc gacagtgaag tcagtttcct
    22201 gacgccatcc ggtcagcgtg ccgagctggc gatcccacaa ctcacctggc tgaacgatcc
    22261 acgtcgacac cgtgcggaag gcctggtaag cctctccagc cttaccggac agcacggcgt
    22321 gatgcaggtg cgcatggatt tgcgcgatga tgaggggttg ttaagcaatg gtcgcgtctg
    22381 gctacaggcg gatgacatcg acctgaagcc gtggctcggt aaatggatgc aggacaatat
    22441 tgctctggaa acggcacagt tctcccttga aggctggatg acgatcgaca aaggcgatgt
    22501 aaccggcggt gacgtctggc tgaaacaggg cggtgccagc tggttgggcg agaagcaaac
    22561 gcatacgctg tcggtggata atctgaccgc gcatattacg cgtgaaaatc cgggctggca
    22621 gttctctatt cccgatacac ggatcacgat ggacggcaaa ccctggccga gcggagcatt
    22681 gacgctggcc tggataccgg aacaggacgt tggcggcaaa gacaataaac gcagtgacga
    22741 actccggatt cgcgccagta atctggagct ggcaggcctg gagggcatac gcccgctggc
    22801 cgcgaaactt tcacctgcac tgggtgatgt ttggcgctcc acacaaccga gcggcaagat
    22861 taacactctg gcgctggata tcccgcttca ggcggcagac aagacccgtt ttcaggcatc
    22921 gtggagcgat ctggcctgga agcaatggaa attattaccg ggtgcggaac acttctccgg
    22981 gacgctttcc ggcagcgttg aaaatggttt gcttaccgcg tcgatgaagc aggcaaagat
    23041 gccttacgaa acggtattcc gtgcgccact ggaaatcgcc gacggccagg caactataag
    23101 ctggctgaac aatgacaaag gtttccagct ggatgggcgt aatattgacg ttaaagccaa
    23161 agccgtccat gcgcgcggcg gttttcgtta cctgcaacct gctaacgatg aaccctggct
    23221 gggtattctg gctggcatca gtaccgatga tggttcacaa gcctggcact atttcccgga
    23281 aaacttgatg ggtaaagacc tggttgatta cttaagtggc gcgattcagg gcggtgaagc
    23341 ggataacgcg acgctggttt atggtggcaa tccgcaactc ttcccctata aacacaacga
    23401 aggtcagttt gaagtgctgg tgccgctgcg caacgcgaag tttgccttcc agccggactg
    23461 gcctgcatta actaaccttg atattgaact ggactttatt aacgacggtt tatggatgaa
    23521 aaccgatggc gttaatctgg gcggcgtgcg cgcgagtaat ctcaccgcag tgatccctga
    23581 ctactcgaaa gaaaaactgc tgattgacgc tgacattaaa ggtccgggta aagccgttgg
    23641 cccttacttt gatgagacac cgctgaaaga ttctctgggt gcgaccctgc aagaactcca
    23701 gctcgacggc gatgtgaatg ctcgcttaca tcttgatatc ccgctgaacg gcgagctggt
    23761 aaccgcgaaa ggtgaagtga cgctgcgtaa taacagtctg tttatcaaac cactcgacag
    23821 caccctgaaa aatttgagcg gtaaattcag ctttatcaat ggcgatctgc aaagtgaacc
    23881 actgacagca agctggttta atcagccgtt gaacgtggat ttttccacca aagaaggggc
    23941 aaaagcctac caggttgcgg tgaatctcaa cggtaactgg caaccggcga aaaccggcgt
    24001 tctgcctgaa gcggtgaacg aagcattgag tggcagcgtg gcgtgggatg gtaaagtggg
    24061 cattgatctg ccttatcatg ctggcgcgac ctataacgta gagctgaacg gcgatctgaa
    24121 taatgtgagt agtcacttac cttcaccgtt agccaaacct gcgggtgaac cactagcggt
    24181 aaacgttaag gttgatggca atctcaacag ctttgaatta accggacagg ctggtgcgga
    24241 taaccatttc aatagccgct ggttgcttgg tcaaaagctg acgctcgatc gtgctatttg
    24301 ggcggcagac agtaaaacgc tcccgccgtt gccggaacaa agtggcgttg aactcaatat
    24361 gccgccgatg aatggtgccg agtggctggc cctgttccag aaaggcgctg cggagagtgt
    24421 cggtggtgca gcgagtttcc cacaatacat aacgttacgt acgcctatgt tgtcactggg
    24481 aaatcagcaa tggaataacc tgagtattgt ttcgcaaccg acggcaaatg gcaccctggt
    24541 tgaggcgcaa gggcgtgaaa tcaacgccac gctagcgatg cgtaataacg cgccgtggct
    24601 ggcgaatatc aaatatcttt attacaaccc gagcgtggcg aaaactcgtg gtgattcaac
    24661 gccgtcatca cctttcccga caacggagcg cattaacttc cgtggctggc cggacgcaca
    24721 aatacgatgc acagagtgct ggttctgggg gcaaaaattc ggtcgcattg acagtgatat
    24781 caccatttct ggcgatacgt taacgctgac caatggactg attgatactg gtttctcgcg
    24841 gcttactgcc gatggtgaat gggttaataa tccggggaat gaacgtacct cgctgaaagg
    24901 aaaactgcgc gggcagaaaa ttgatgccgc cgcagaattt tttggtgtca cgacgcccat
    24961 acgccagtcg tcatttaatg tggattacga tttacactgg cgcaaagcac cctggcagcc
    25021 agatgaagcg acgttgaatg gcatcattca tactcaactg ggtaaaggcg aaattaccga
    25081 aatcaatacc ggacatgccg ggcaattgct gcgcttattg agcgtagatg ccctgatgcg
    25141 taagctgcgt tttgatttca gagacacttt tggcgaaggg ttctattttg actccattcg
    25201 cagcaccgcg tggattaaag acggggttat gcacaccgac gacacgctgg tggatggcct
    25261 ggaggcggat atcgccatga aagggtcggt aaatctggta cgtcgcgacc tgaatatgga
    25321 agcggttgtc gcaccagaga tttctgcgac ggtgggcgtg gctgcggctt ttgcggttaa
    25381 ccccattgtt ggcgcggcag tttttgccgc tagtaaagtg ctggggccgc tgtggagcaa
    25441 agtctccatt ttgcgctatc acatttcggg tccgctggac gatccgcaaa tcaacgaagt
    25501 gttgcgccaa ccgcgtaaag aaaaagcgca atgatttgac gagggcgcgt aattgcccca
    25561 atctcatatg ataatcgttg ccaaaggcca acgagccaga acataaccgt aggccggata
    25621 gggcgttcat gccgcatccg gcagccgtaa aaaatcctct actgcagtaa ctaacgagta
    25681 gcaaaaacga tgagtctgaa cctggtaagt gaacaattgc tagcggcgaa cagcctgaaa
    25741 catcaggact tgttcgcgat cctcggtcaa ctggccgaac gtcgccttga ttatggcgat
    25801 ctctattttc agtcgagcta tcacgaatcc tgggttttag aagaccgcat tattaaagat
    25861 ggttcttaca acatcgatca gggcgttggc gtgcgtgcaa tcagcggtga aaaaaccgga
    25921 tttgcttacg ctgaccaaat cagcctgctg gcgctggaac agagtgcgca agcggcgcgc
    25981 accatcgtcc gtgatagtgg tgatggcaaa gtacagacgc tgggcgcggt agagcatagc
    26041 ccgttgtata cctcggtaga tccgctgcaa agcatgagcc gtgaagagaa gctggatatc
    26101 ctgcgtcgcg tcgataaggt tgctcgcgaa gcggacaagc gcgtacagga agtgactgcc
    26161 agcctcagtg gtgtctatga attaattctg gttgcggcca ccgacggcac gctagcggcg
    26221 gatgtccgtc cgctggtgcg tctttccgtg agcgttctgg tcgaagaaga tggcaaacgc
    26281 gaacgcggtg ccagtggcgg cggcggtcgt tttggttatg agttcttcct tgccgatctt
    26341 gacggcgaag tccgtgcgga tgcatgggca aaagaagcag tgcgtatggc gctggtcaat
    26401 ctttctgccg ttgctgcacc agcgggcacc atgccggtag tacttggcgc aggttggccg
    26461 ggcgtgctgt tgcatgaagc ggtaggtcac ggtctggaag gcgacttcaa ccgccgcggc
    26521 acttcagtat ttagtgggca ggtcggggag ctggtggctt cagaactgtg taccgtggtt
    26581 gatgacggca cgatggtcga tcgccgtggt tcggtggcga ttgatgacga aggtacgcca
    26641 ggccagtaca acgtgctgat tgagaacggc attctgaaag gctacatgca ggataaactc
    26701 aacgcgcgtt tgatggggat gacgccgact ggcaacggtc gccgtgaatc ctacgcccat
    26761 ctgcccatgc cgcgtatgac taacacctat atgctgccgg gtaaatcgac cccgcaggaa
    26821 attattgaat ccgttgagta cggtatctat gcaccgaact ttggtggcgg tcaggtggat
    26881 atcacctccg gcaaattcgt tttctccact tcagaagcat atctgattga aaacggtaaa
    26941 gtaacgaagc cggtgaaagg cgcaacgttg attggttccg gtatcgaaac catgcagcag
    27001 atttcgatgg ttggcaacga cctgaaactg gataacggcg tgggtgtctg cggtaaagaa
    27061 gggcaaagtt tgccggttgg cgtgggccag ccaacgctga aagttgataa cctgactgtt
    27121 ggcggtactg cgtaataatt tcattatttt cagaaggata attaatcttt ctacgtgcac
    27181 gaacggtccc ctcgctcctc tggggttagg gtgaggggaa cccgttggca caggtttgta
    27241 gaacgtaaca gtacaatatg aattacttct ctttcccgcg cccgtgcatc tcctgaaaca
    27301 atttaccgac ctcaacaaaa taatccgtca gcgagttgat cacgacctgt accttcagcg
    27361 gcagcttatc tttttcggta tataacgcat aaaccgggcg tggatctgac tgataacgcg
    27421 gcagcaggat ctccagctca ccgcgattga tctcgttgat cacccacatc agcggcacgt
    27481 aggcgatccc ggcacccgcc gtcagccagc gcaccagcgt catcggatca ttagtcacaa
    27541 atcttccttg tgggatcagg cgagtcgaga tcccttccgg tgcgatcagt tcaaattcat
    27601 tgtcgggccg cacgctgtat tcaagccatg aatgactact caaatcggcg ggtttttccg
    27661 gtatgccgta ttgtgtgaga tagcttttcg cggcgcacac caccatcggc atcgcgccca
    27721 gacggcggga aaacaggctg gaatcctgca acgcgccgac gcggatcacc acatccagac
    27781 cgtcggcaat caggtcgggg gctggaattc cggtaaccag attgacgctc aaacctgggt
    27841 attctttcag catttttgct gtcagcccgg cgagaacatt ttgtgccata gttgaagaac
    27901 agccaatgcg cagcgtcccg atgggggtgt tattgaaggc atacagttgc tcatgaacat
    27961 cctgcacttc atgaagcatc cgacggcagc cctggtagta aattctgccg gcttcggtca
    28021 ggccaatgct gcgtgtgcta cggtttaaca gctttacctg caactcatct tccagttttg
    28081 acaccgtctg actgatggac gaaacgctca tctgtagctg tctggcggcg gcggtaaaag
    28141 agccaaattc aactactttg gcaaacaccg acatgcgttt tagtcgttcc attattcact
    28201 ctgacttaaa agtgatttag atcacataat atagataaca gcataacagt tacgctaata
    28261 tattaaatat caatctacag caatgttgct ctcgcccggc tttccatgcc cttctctgcg
    28321 gcgacagatg ctgaaaataa taacgcctgc tctctcttta caaccaaggt caacatgagt
    28381 ctgtttcccg ttatcgtggt gtttggactg tccttcccac cgatattttt tgaattgctt
    28441 ttatcactgg cgattttctg gctggtgcgc cgggtacttg tgccaacagg tatctacgac
    28501 tttgtctggc atccggcgtt gttcaacacc gcgctctatt gctgcttgtt ttatttgata
    28561 tcgcgactgt tcgtttgagg ttgaagtgaa aacactaata agaaaattct cccgtacggc
    28621 catcacggtc gtattagtca ttctagcctt catcgcaatt tttaatgcct gggtctatta
    28681 caccgaatcc ccctggacgc gtgacgcgcg ctttagcgct gacgtcgttg cgatcgcgcc
    28741 ggacgtttct ggactcatta cccaggtgaa tgttcatgat aaccagctgg tgaaaaaagg
    28801 acagatactg ttcaccatcg accagccgcg ctatcaaaag gcgcttgagg aagcgcaagc
    28861 cgatgttgct tattatcagg tactggcaca ggagaaacgc caggaggccg gacgtcgtaa
    28921 ccgtctcggt gtgcaggcga tgtctcgcga agagatcgac caggccaaca acgtactaca
    28981 aacggttctg catcagttag cgaaagcgca ggcgacccgc gatctggcaa aactggatct
    29041 tgaacgcacg gtgatccgcg cgccagcaga tggctgggtg accaacctca acgtctatac
    29101 cggtgagttt attactcgag gatcaacggc ggttgcgctg gtgaaacaga actccttcta
    29161 tgtactggcc tatatggaag aaactaagct ggaagggggg cgtccggggt atcgtgcaga
    29221 gatcacgccg cttggcagta acaaagtgct gaaagggact gttgatagtg ttgccgcagg
    29281 ggtcaccaac gccagcagca cgcgtgacga caaagggatg gcgactatag actctaatct
    29341 tgaatgggtg cgtcttgcgc aacgtgttcc ggttcgtatt cgtctcgaca accagcaaga
    29401 gaacatctgg cctgcgggca ccactgctac agtggtggtc actggcaaac aagatcgcga
    29461 cgaaagccag gattcgttct tccgtaaaat ggcccatcgc ctgcgtgagt ttggttaatc
    29521 acgatgggta ttttctccat tgctaaccaa catattcgct ttgcggtaaa actggcgacc
    29581 gccattgtac tggcgctgtt tgttggcttt cacttccagc tggaaacgcc acgctgggcg
    29641 gtactgacag cggcgattgt tgccgccggt ccggcctttg ctgcgggagg tgaaccgtat
    29701 tctggcgcta ttcgctatcg tggctttttg cgcatcatcg gcacatttat tggctgtatt
    29761 gccggactgg tgatcatcat tgcgatgatc cgcgcaccat tattgatgat tctggtgtgc
    29821 tgtatctggg ccggtttttg tacctggata tcctcgctgg tacgaataga aaactcgtac
    29881 gcgtgggggc tggccggtta taccgcgctg atcattgtga tcaccattca gccggaacca
    29941 ttgcttacgc cgcagtttgc cgtcgaacgt tgtagcgaga tcgttatcgg tattgtgtgt
    30001 gcgattatgg cggatttgct cttttctccg cgatcgatca aacaagaagt agatcgagag
    30061 ctggaaagtt tgctggtcgc gcaatatcaa ttaatgcaac tctgtatcaa gcatggcgat
    30121 ggtgaagttg tcgataaagc ctggggcgac ctggtgcgac gcaccacggc actacaaggc
    30181 atgcgcagca acctgaatat ggaatcttcc cgctgggcgc gggccaatcg acgtttaaaa
    30241 gcgatcaata cgctatcgct gacgctgatt acccaatcct gcgaaactta tcttattcag
    30301 aatacgcgcc cggaattgat cactgatact ttccgcgaat tttttgacac gccggtagaa
    30361 accgcgcagg acgtccacaa gcagctcaaa cgcctgcgga gagttatcgc ctggaccggg
    30421 gaacgggaaa cgcctgtcac catttatagc tgggtcgcgg cggcaacgcg ttatcagctt
    30481 ctcaagcgcg gcgttatcag taacacaaaa atcaacgcca ccgaagaaga gatcctgcaa
    30541 ggcgaaccgg aagtaaaagt agagtcagcc gaacgtcatc atgcaatggt taacttctgg
    30601 cgaaccacac tttcctgcat tctgggcacg cttttctggc tgtggacggg ctggacttcc
    30661 ggcagtggtg caatggtgat gattgcggtc gtgacgtcac tggcaatgcg tttgccgaat
    30721 ccacgcatgg tggcgatcga ctttatctat gggacgctgg ccgcgctgcc gttagggctg
    30781 ctctactttt tggtgattat ccctaatacc caacagagca tgttgctgct gtgtattagc
    30841 ctggcagtgc tgggattctt cctcggtata gaagtacaga aacggcgact gggctcgatg
    30901 ggggcactgg ccagcaccat aaatattatc gtgctggata acccgatgac tttccatttc
    30961 agtcagtttc tcgacagcgc attagggcaa atcgtcggct gtgtgctcgc gttcaccgtt
    31021 attttgctgg tgcgggataa atcgcgcgac aggactggac gtgtactgct taatcagttt
    31081 gtttttgccg ctgtttccgc gatgactacc aatgtggcac gtcgtaaaga gaaccacctc
    31141 ccggcacttt atcagcagct gtttttgctg atgaataagt tcccagggga tttgccgaaa
    31201 tttcgcctgg cgctgacgat gattatcgcg caccagcgcc tgcgtgatgc gccgatcccg
    31261 gttaacgagg atttatcggc gtttcaccga caaatgcgcc gcacagcaga ccatgtgata
    31321 tctgcccgta gcgatgataa acgtcgtcgg tactttggcc agttgctgga agaactggaa
    31381 atctaccagg aaaagctacg catctggcaa gcgccaccgc aggtgacgga accggttcat
    31441 cggctggcgg ggatgctcca taagtatcaa catgcgttga ccgatagtta agtcaaaacc
    31501 gacgccaaaa gcgtcggttt tttcatggct atacttagcg atgcacggca gaactcgccg
    31561 cgaaacgtga cggtggcaac agatgaatat ttataccttt gattttgatg agattgagag
    31621 tcaggaggat ttttatcgtg actttagcca aacctttggt ctggctaaag ataaggtacg
    31681 cgatctcgac tcactatggg atgtgttaat gaacgatgtc ctgccgctac cacttgagat
    31741 tgaatttgtt catctgggag agaaaacgcg tcgccgtttt ggcgcgttaa tattgctgtt
    31801 tgatgaggca gaggaagagc tggaagggca tttgcgtttt aatgttcgtc attagcgcaa
    31861 aaaaaagccc ccgaaccggg ggcaatatcg tcggacaaga cgatgagggt ttatttgtac
    31921 agttcagccg tagcgtgcca ggtgtcaccg ctacgagctt cagtaatctg gtaggccgtt
    31981 gcgcctttct cttccgcttt tttgttcagc atttcacgca tatccattgg cgaagacgcc
    32041 acaccactta cggatacggt cccgattgct tcacgatttt gtgcttgtgc agtatcaatg
    32101 gagtcggcag cgaatgcacc gaaagagaga acagaaagta cgcttaatgc agctacagtg
    32161 gttttgattt tcatgatttt tacctcgaca taatctttta gctgggcctt tgtttcgtga
    32221 cccatatcac aaaatcaagc atacactaat tattgaacta attaatacca cgctaactat
    32281 ttcagttgat actaagtgtt aaaaatgtga attttaataa caaaaaagaa ttaaaaacac
    32341 ggttatttat tgttaattct a
//
LOCUS       DAZPLI010000033        30896 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZPLI010000033 DAZPLI010000000
VERSION     DAZPLI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30896
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.033"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            496..1011
                     /locus_tag="ACHUU3_001474"
     CDS             496..1011
                     /locus_tag="ACHUU3_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR2081581.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            1229..1957
                     /gene="artP"
                     /locus_tag="ACHUU3_001475"
     CDS             1229..1957
                     /gene="artP"
                     /locus_tag="ACHUU3_001475"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR2081582.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            1975..2706
                     /gene="artI"
                     /locus_tag="ACHUU3_001476"
     CDS             1975..2706
                     /gene="artI"
                     /locus_tag="ACHUU3_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR2081583.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            2713..3429
                     /gene="artQ"
                     /locus_tag="ACHUU3_001477"
     CDS             2713..3429
                     /gene="artQ"
                     /locus_tag="ACHUU3_001477"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR2081584.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            3429..4097
                     /gene="artM"
                     /locus_tag="ACHUU3_001478"
     CDS             3429..4097
                     /gene="artM"
                     /locus_tag="ACHUU3_001478"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR2081585.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            4389..5120
                     /gene="artJ"
                     /locus_tag="ACHUU3_001479"
     CDS             4389..5120
                     /gene="artJ"
                     /locus_tag="ACHUU3_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR2081586.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(5295..6422)
                     /gene="rlmC"
                     /locus_tag="ACHUU3_001480"
     CDS             complement(5295..6422)
                     /gene="rlmC"
                     /locus_tag="ACHUU3_001480"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR2081587.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(6463..6951)
                     /locus_tag="ACHUU3_001481"
     CDS             complement(6463..6951)
                     /locus_tag="ACHUU3_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR2081588.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(7011..7856)
                     /gene="potI"
                     /locus_tag="ACHUU3_001482"
     CDS             complement(7011..7856)
                     /gene="potI"
                     /locus_tag="ACHUU3_001482"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR2081589.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(7853..8806)
                     /gene="potH"
                     /locus_tag="ACHUU3_001483"
     CDS             complement(7853..8806)
                     /gene="potH"
                     /locus_tag="ACHUU3_001483"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR2081590.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(8816..9949)
                     /gene="potG"
                     /locus_tag="ACHUU3_001484"
     CDS             complement(8816..9949)
                     /gene="potG"
                     /locus_tag="ACHUU3_001484"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR2081591.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(10044..11156)
                     /gene="potF"
                     /locus_tag="ACHUU3_001485"
     CDS             complement(10044..11156)
                     /gene="potF"
                     /locus_tag="ACHUU3_001485"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR2081592.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(11507..11983)
                     /locus_tag="ACHUU3_001486"
     CDS             complement(11507..11983)
                     /locus_tag="ACHUU3_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR2081593.1"
                     /translation="MTSLVVPGLDTLRQWLNDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(12071..12973)
                     /gene="rimK"
                     /locus_tag="ACHUU3_001487"
     CDS             complement(12071..12973)
                     /gene="rimK"
                     /locus_tag="ACHUU3_001487"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR2081594.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(13034..13756)
                     /gene="nfsA"
                     /locus_tag="ACHUU3_001488"
     CDS             complement(13034..13756)
                     /gene="nfsA"
                     /locus_tag="ACHUU3_001488"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR2081595.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(13740..14027)
                     /locus_tag="ACHUU3_001489"
     CDS             complement(13740..14027)
                     /locus_tag="ACHUU3_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR2081596.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            14187..14444
                     /gene="grxA"
                     /locus_tag="ACHUU3_001490"
     CDS             14187..14444
                     /gene="grxA"
                     /locus_tag="ACHUU3_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR2081597.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(14474..14851)
                     /locus_tag="ACHUU3_001491"
     CDS             complement(14474..14851)
                     /locus_tag="ACHUU3_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR2081598.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            15121..16806
                     /locus_tag="ACHUU3_001492"
     CDS             15121..16806
                     /locus_tag="ACHUU3_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR2081599.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(16981..17517)
                     /gene="rcdA"
                     /locus_tag="ACHUU3_001493"
     CDS             complement(16981..17517)
                     /gene="rcdA"
                     /locus_tag="ACHUU3_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR2081600.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            17601..18809
                     /locus_tag="ACHUU3_001494"
     CDS             17601..18809
                     /locus_tag="ACHUU3_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2081601.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            18809..19624
                     /gene="ybjI"
                     /locus_tag="ACHUU3_001495"
     CDS             18809..19624
                     /gene="ybjI"
                     /locus_tag="ACHUU3_001495"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR2081602.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            19710..19994
                     /gene="ybjH"
                     /locus_tag="ACHUU3_001496"
     CDS             19710..19994
                     /gene="ybjH"
                     /locus_tag="ACHUU3_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR2081603.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTGDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(20035..21262)
                     /gene="mdfA"
                     /locus_tag="ACHUU3_001497"
                     /pseudo
     CDS             complement(20035..21262)
                     /gene="mdfA"
                     /locus_tag="ACHUU3_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            21547..22143
                     /gene="ybjG"
                     /locus_tag="ACHUU3_001498"
     CDS             21547..22143
                     /gene="ybjG"
                     /locus_tag="ACHUU3_001498"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR2081604.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            22201..22959
                     /gene="deoR"
                     /locus_tag="ACHUU3_001499"
     CDS             22201..22959
                     /gene="deoR"
                     /locus_tag="ACHUU3_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR2081605.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(23006..24208)
                     /gene="dacC"
                     /locus_tag="ACHUU3_001500"
     CDS             complement(23006..24208)
                     /gene="dacC"
                     /locus_tag="ACHUU3_001500"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR2081606.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            24455..25080
                     /gene="gstB"
                     /locus_tag="ACHUU3_001501"
                     /pseudo
     CDS             24455..25080
                     /gene="gstB"
                     /locus_tag="ACHUU3_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(25077..26192)
                     /gene="yliI"
                     /locus_tag="ACHUU3_001502"
     CDS             complement(25077..26192)
                     /gene="yliI"
                     /locus_tag="ACHUU3_001502"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFR2081607.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(26303..26686)
                     /gene="bssR"
                     /locus_tag="ACHUU3_001503"
     CDS             complement(26303..26686)
                     /gene="bssR"
                     /locus_tag="ACHUU3_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR2081608.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            26899..28224
                     /gene="rimO"
                     /locus_tag="ACHUU3_001504"
     CDS             26899..28224
                     /gene="rimO"
                     /locus_tag="ACHUU3_001504"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR2081609.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(28271..29592)
                     /locus_tag="ACHUU3_001505"
                     /pseudo
     CDS             complement(28271..29592)
                     /locus_tag="ACHUU3_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(29600..>30896)
                     /locus_tag="ACHUU3_001506"
     CDS             complement(29600..>30896)
                     /locus_tag="ACHUU3_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFR2081610.1"
                     /translation="SIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLA
                     VGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENV
                     QTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFS
                     EFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVS
                     AERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPW
                     QLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISID
                     DFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVE
                     SEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         7791 a         7966 c         7483 g         7656 t
ORIGIN      
        1 ccacaattgt agcataaata atattatcga gagtcaaagc tgggactatc aaatttcgct
       61 cattttcaaa aatacaacgt aattttggca atcatcttaa tcgcaggatc tcttatcgca
      121 tgtgaaaaat tttgtaatga ttagacaata accgcgttca tttatcacgc gttataaatg
      181 tgaaactggc ttcttgcaga tgctcttaac ctctacagga ggatttatac caccatttca
      241 gaactatcat atttacctta aatgatcatt tcagccatat aacacgctca acctcacaaa
      301 tggtttacac tgtctattgt ttgggtaatt taagtaagtc agctacgagc aaccttgagt
      361 ggatagccag caatggactc aaacccaatc aggtgttcag attgtttcag aattgtatta
      421 aaaacaatgt attaaataat ctgatagcgt ttggtcgtag tgtgacgcag acagcaagct
      481 aataaggaaa tgattatgcg ctaccgttca ctatctctac ttattccatg tgcactgctg
      541 ctgagtgcct gtaccaccgt cacgccagct tataaagata acggtccccg caccggttcc
      601 tgtgtccagg gggggcctga cagcgtggct caacaattct atgactaccg cattcaacat
      661 cgcagcaatg acattaccgc cctgcgcccc tatttgagtg acaaactggc aacccttctt
      721 agcgatgcca gccgcgataa tagtcatcgt gaattgttaa gcagcgatcc gttctccagt
      781 cgaaccacct tgccagatag cgcccacgtt gccagtgcat cgactatccc taatcgtgat
      841 gcccgtaaca ttccattgcg cgtagatctc aaacagggtg accagggttg gcaggatgaa
      901 gtgttaatga ttcaggaagg ccagtgttgg gtcatcgacg atgtgcgcta cttaggcggc
      961 agcgttcatg ccacggcggg tacgctccgt cagtccatcg agaatcgcta acgccttgat
     1021 caaccaataa accccgtaaa ttgtccggca tttcgtgagg aatgccgaca tttatgctcg
     1081 ccgaccaccg cccccgttat tttgtgctat gtttattgaa taatgcgctt tgcttttaac
     1141 ttttaaagca gaaatattgc ataattattc tgtcaaaggt actatcttcg acctcaatag
     1201 ctatcagact gccagtatac gagtgtcaat gagtattcaa ttaaacggca ttaattgctt
     1261 ctacggcgcg catcaggcgc tgttcgatat cacgctggat tgcccacagg gcgaaacgct
     1321 ggtgttactt ggccccagcg gcgcgggtaa aagctcgctg ctgcgtgtac tcaatctgct
     1381 tgagatgcca cgctccggta cgctcaacat tgcaggcaac catttcgatt tcaccaaaac
     1441 accctctgac aaagcgattc gcgatttgcg tcgtaacgtt ggcatggtgt ttcagcaata
     1501 caacctgtgg ccgcatctga ccgtgcagca aaacctgatt gaagcgccct gccgtgtact
     1561 ggggttgagt aaagatcagg cgctggcccg tgcagaaaaa ctgctggaac gtctgcgtct
     1621 caaaccttat agcgatcgtt acccgctgca tctttctggt ggtcagcagc agcgtgttgc
     1681 tattgcccgt gcgttgatga tggaaccgca ggtactgctg ttcgatgaac cgaccgccgc
     1741 actggacccg gaaattacgg cacaaatcgt cagcatcatt cgtgagctgg cagaaacgaa
     1801 tattacccag gtgatcgtca cccacgaagt tgaagtggcg cgtaaaaccg ccagccgagt
     1861 ggtgtatatg gaaaatggtc atatcgtaga acaaggcgac gcgagctgct ttaccgagcc
     1921 gcaaaccgaa gcatttaaaa actatctctc tcactaagat tcgggataac aacaatgaaa
     1981 aaagttctga ttgccgcgtt aattgcaggt tttagtcttt ccgccacagc tgccgaaacc
     2041 attcgttttg ctaccgaagc atcctatcct ccgtttgaat cgattgatgc aaacaaccag
     2101 atcgttggtt ttgacgtcga cctggcacaa gcgctgtgta aagagattga tgcaacctgc
     2161 actttctcta accaggcgtt tgacagcctg atcccaagcc tgaaattccg tcgcgtagaa
     2221 gccgtgatgg cgggcatgga tatcactccg gagcgtgaaa agcaggtgct gtttaccacc
     2281 ccgtactatg acaactctgc cctgtttgtg ggtcagcaag gcaaatacac cagtgttgat
     2341 cagctgaaag gcaaaaaagt cggcgtacag aacgggacga cacaccagaa attcattatg
     2401 gataagcacc cggaaatcac taccgttccg tatgacagct accagaacgc aaaactggat
     2461 ctgcaaaacg ggcgtatcga cggcgtcttc ggtgacaccg cagtggtcac tgagtggctg
     2521 aaagataacc cgaaactggc ggcggtgggc gacaaagtga ccgataaaga ttacttcggc
     2581 actggcctcg gcatcgcggt acgtcagggc aacactgagc tgcagcagaa actcaacact
     2641 gcgctggaaa aagtgaagaa agatggcact tacgaaacca tctacaacaa atggttccag
     2701 aagtaattcc tgatgaatga attttttcct ttagcaagcg ccgccgggat gaccgtcggc
     2761 cttgccgttt gtgcattgat tgtcgggctg gcgctggcga tgttctttgc ggtatgggag
     2821 tcggcaaaat ggcgtcctgt cgcgtgggca ggttcagcgc tggtaaccat tctgcgtggc
     2881 ctgccagaaa ttctggtggt gctgtttatc tattttggct cctcgcagct gctgctgacg
     2941 ctttcggatg gcttcactat caatcttggg ttcgtgcaga tcccagtgca gatggacatt
     3001 gagaacttcg acgttagccc gttcctttgt ggtgtcatcg ctctgtcact gctgtatgcc
     3061 gcctatgcct cgcaaacgct tcggggcgcg ttgaaagcgg tgccggtggg tcagtgggag
     3121 tctggtcagg cgctggggct gtcgaaatcg gctatctttt tccgtctggt gatgccgcag
     3181 atgtggcgtc atgcgctgcc tggcctcggt aaccagtggc tggtgctgct gaaagatacc
     3241 gcgctggtca gtttgattag tgtgaatgat ttaatgctgc aaacaaaaag catcgctact
     3301 cgtacccagg aaccatttac ctggtacatt gtggcggcgg cgatttacct ggtgatcacc
     3361 ctgctcagtc agtacattct caaacgcatt gacctgcgcg cgacacgttt tgagcggagg
     3421 cccagctaat gtttgagtat ttacccgaac tgatgaaagg gctacacacc agcctgacgc
     3481 taaccgttgc ctcgctgatt gtggcactga ttctggcatt gatttttacc atcatcctga
     3541 cgctgaaaac gccggtgctg gtgtggctgg tgcggggtta tatcacgctg tttaccggta
     3601 cgccgctgct ggtgcagatc ttcctgattt attacgggcc gggccagttt ccgacgttgc
     3661 aggagtatcc ggcactgtgg catttgttgt cagaaccgtg gttatgtgcg ctgattgcgt
     3721 tatcgctgaa cagtgcggcg tataccacgc agctgtttta cggtgcaatt cgtgcgatcc
     3781 cagaaggtca gtggcagtcc tgtagcgccc tgggaatgag caaaaaagat acgctggcgc
     3841 tcctgctgcc gtatgccttt aaacgctcgc tctcttctta ttccaacgaa gtggtgctgg
     3901 tgttcaaaag tacctctctg gcttacacca ttacgctgat ggaagtgatg ggatacagcc
     3961 agttgttgta cggacgcacc tacgatgtaa tggtgttcgg tgcggcaggg attatttacc
     4021 tggtcgtcaa cggcctgctg acgctgatga tgcgtctgat cgagcgcaaa gcactggcat
     4081 tcgaacggcg gaattaatgt agctgcgtac attcgccggg ggggtttgta ggtctgataa
     4141 gacgcgccag cgtcgcatca gacatcagca cggtgcctgt gccggatgcg gcgtggacgc
     4201 cttatccggc ctacatagtg cataaattct tataatgaag acggacaact cactaagtta
     4261 tccgtctttt tttatttcat ttaaattatt taatcatatt tattgcatat aaattcactt
     4321 gatggcattg ttatcccatg ccgcagacac ggccaaaaat cataagattg acagacggga
     4381 gttccatcat gaaaaagtta gttcttgccg ctttacttgc ttcctttact ttcggtgctt
     4441 ctgccgcaga gaaaatcaat tttggcgttt cagccaccta tccacccttt gaatctatag
     4501 gtgctaataa tgagattgtc ggctttgata tcgatctggc aaaagccttg tgcaaacaaa
     4561 tgcaggcaga atgtactttt actaatcacg cgttcgacag cctgatcccg tcactgaaat
     4621 tcagaaaata tgactccgta atctccggta tggatatcac gcccgagcgt agcaagcagg
     4681 tatcgtttac cacgccctac tatgaaaact cagccgtcgt gattgccaaa aaagatacct
     4741 acaaaacgtt tgccgatctg aaaggcaaac gtattgggat ggaaaacggc accacgcacc
     4801 agaaatatat tcaggatcag caccccgaag tgaaaactgt ctcctatgac agttatcaga
     4861 atgcctttat cgatctgaaa aatggtcgta ttgatggggt atttggtgac acagcggtgg
     4921 taaacgaatg gctgaaaacc aatccgcaac tgggcgttgc tactgaaaaa gtgaccgatc
     4981 cgcaatactt cggcaccggt ctgggcatcg ctgtacgtcc ggataacaaa gctctgctgg
     5041 aaaaactgaa taacgcgctg gcagcaatta aagctgacgg tacatatcag aaaatcaatg
     5101 accagtggtt cccgcagtaa ttgagactga tgacaaaagt aagattgcct gatgcgctgc
     5161 gcttatctgg cccacatcaa ctctgcaatg tattgattta gcatgatttt gtaggccgga
     5221 taaggcgtac acgccgcatc cggcatgaac aaagcgcacc tgatcagcaa tctgaagccg
     5281 cacctgcggc ttttttattg cttcaccagc agcgtcatca cttcatagtg cgcggtgtgc
     5341 gggaacatat caaaaagctg tacccgttcg atgcggtaac ctggcagttc gcggatatct
     5401 ttcgccatgg tttgggcgtt acagctggag tagataataa aacgcggtgc catcgttgag
     5461 agataatcac acagcggttt accaatgccg cggcgcggcg ggttaaccag caccagctcc
     5521 ggcacatccc cctgagcggt ggcaaactga gtggagtcca gcgcctgaaa ttgcaaacgc
     5581 gttaagccca gttcagcggc tgactgcttt gcacaggcaa tggcctctga tgcaatttcg
     5641 atcccggtta actgcatgtc aggcgtcgcg cagtgtaaac caaagccacc cacaccgcag
     5701 aacagatccc acatatgttt aactggcagc tgccgtaccc agtcgcgcgc ggtagcgtac
     5761 agctggctgg cgaccgccgg attagtctgg aagaaacttt gcggacggat ccacagcggt
     5821 acgtcattaa aacgctctgc cagcgcctgt tgttcagtca ggtagatctc cgtctcccct
     5881 tccataatcg ccatatgtac cggctgaata ttgacggtaa taactttcag ttgcggtagt
     5941 tgttcctgta accacggcag cgccttacgc agttgtgcca gtttggtttc agaacgcagt
     6001 acaaagcgca gcatcatgcc gccatcgctc tggctttcag tcagcagaat gtatttcagt
     6061 tcgccacgct tacgcgccac gttgtagggc gttaaccccg cacgggcgat aaacggtttt
     6121 agcgccgcaa aaacgggcgc aaaagaggca ggataaagcg ggcagtcaca aaggtcttcc
     6181 ggcgtgccat cgcgatgcag cataccgagc agtggttttt caacgctacc actcaccacc
     6241 attttggctt tattacgaaa cccttgttcc gggcctgaca ccggcgcgca ccattcctca
     6301 accggaaaat cggcgagcag atttttaaga tcggcggttt tagcggagag ttgctctgga
     6361 atcggctgcg ttatccactg acaggaacga cagcgacccg cgtcgtaaag tgcgcactgc
     6421 atacattgac cttcacatca tcaggggcga cgattataca cattattgca actggaagaa
     6481 ccgccgactg gtcgagggaa cgaacagcag catcaggatg agcatatccg gcagcttctg
     6541 cagcatcagg ctatggaaga tttcacgttt tgattcaccg ggaatgctga acagctccgg
     6601 ataaccgtac cccagcgaag ccgcccacaa gtaacttgcg gcggtgattt gtgtcagcag
     6661 atatagccag cgcgcccagc gacgcccttt caccagtgaa aacgcacacc agatctcaat
     6721 gaaaaccagc accagactgc ttaaaaagac cagcgttaaa ctccaggttt gtacgctgcg
     6781 atgaatgaac tcgccaatac cgcgcacgcc cagcgtattg aaaatcatca gcacgtcgag
     6841 gccacggatc ataataatgg cgagcgccgc cacctgcacc agcgcaggca cattcaggcg
     6901 agcatgagat gaagatgttt tcttaaaaaa tcccaacgtt tcgtcttcca tgaaaacaat
     6961 gccgcggcat gcgcggcatt atgtagccag gttggcaaat tttagtgtct tcagccatgt
     7021 cttgcacgct ggatatcgcg tatccgctgt ttttctgcgc gagccatcag ataccaggcg
     7081 ataaatccga caattccgac cgcaccgaga attaacgttg caagggcgtt gatttccgga
     7141 ttcactccca tccgcacgct ggaaaagacc agcatcggta acgtggtggc tcccggcccg
     7201 gaaacaaagc tggcgatcac cagatcatca agcgacaaag taaaagccag taaccagcca
     7261 gaaatgatcg cgggcatgat catcggtagc gtaatgacga aaaacacttt cagcggcgtc
     7321 gcaccgagat ccatcgctgc ttcttctatc gagcgatcca gttcccgcaa tcttgaagaa
     7381 ataacgaccg ccacataagc cgtacaaaaa gtgacatgcg ccagccagat ggtgagcata
     7441 ccgcggtccg caggccagcc aatggcatga gcaagcgcga cgaataataa caacagcgac
     7501 aagcccgtga tgacatctgg catcaccagc ggcgcggtga tcataaaggc aaaaccattt
     7561 gatccgcgaa acctgccaaa acgcaccagc accaccgccg caatagtccc gaggatcgcc
     7621 gccgccgttg ccgcacaggc agcaattgtc aggcttaaac caaccgcact catcatcgcg
     7681 tcatcgcgca ataactcacc ataccagcgc gttgaccagc cggcccacac cgtcaccagc
     7741 ttcgagctgt taaacgaata gataaccagc atcagcattg gcgcgtagag aaaggtaaag
     7801 cccagcagca aaatcacaat ccgccagggc gaacgaacta ccggtaaatt attcatccgt
     7861 gttctcccac gcttttttgc tggtgtttgt ggaaccacat tatcggcaca attagcagca
     7921 gcaacatgat gatcgctacc gccgaggcca ccggccagtc gcggttattg aaaaactctt
     7981 gccataacac gcgcccgatc atgatgctgt ccgggccacc gagcagttcc gggatcacaa
     8041 actcgcccac agccgggata aacaccagca tcgatccggc aataatccca cctttagtca
     8101 gcggcacgat cacagtaaag aacgttttca gcggtcgtgc accgagatcc agcgctgctt
     8161 ccaccagcga ataatcaata cgaatcaacg cggtataaat cggcagtacc ataaacggca
     8221 cgtaagcgta aacaatgccg atataaacgg ccagattggt atgcagaatg gtcagcggtt
     8281 gatcgataac ccccagccac agcagaaaat tattcagcac accgttgttt tttaatattc
     8341 ccatccaggc ataaacgcgg atcagaaacg aggtccacga cggcaggatc accagtagta
     8401 ataaaatatt acgggtcgaa ggcttactgt gcgccaccgc ccacgccagc ggatagccga
     8461 tcagtaaaca gcaaaatgtc gaaatcgccg ccacctgtaa cgactggaga taagcatcaa
     8521 aatagagcgg atcgtcggtc agttgcagaa aattaccgag attaagagtg atggaaagtt
     8581 gcccgtcagc ccactccatc agttcggtat aaggtggaat agcgcgcgcc atctctccca
     8641 ggcttatttt aaagacaatc agaaatggca gcagaaacag caagatcaac cagatatata
     8701 gcaacgcaat gaccagtttg cgcccatgct tcatttgcag ctgcgacaac cacagcttaa
     8761 atccgcccgg cggtttcgac tgggcagcag gttcaagtgt actcatcttt gctccttaaa
     8821 ccgtcagcac cacacagctg tccacttccc agcacaaacg cacttcgtcg ccccaggtcg
     8881 gtaacccttt acgatgacga tgggcgtttt gtagctgggc gctgatcatc tgcccacttt
     8941 tcagtcgaac gtgatacacc gaaagatcgc cgagataggc aatgtgtatc acctccccca
     9001 ccgcgaagtt acaaccattg gcgggcggct cttcgcaaag catgattttt tccgggcgca
     9061 gcgctacgtg taccggcacg ttatcgacca ctgaagcatc cgcatcgact ttcagtggat
     9121 gcaccagccc cggcgaatca agcaccaggc catcttcctg acgctctttg agtacacctt
     9181 caaagacatt aaccgaaccg ataaattcag cgctgtagcg ggtagttgga tgctcgtaga
     9241 tctcttccgg ctcgccaatc tggacaaatt tcccgcgatt cataatggcg atacgccccg
     9301 ccatggtcat cgcctcttcc tgatcgtggg tgaccatcac acaagtcaca ccgacgcgct
     9361 ccagaatatc caccacttca agctgcatcc ggtcacgcag ctttttatcc agcgcgccca
     9421 tcggctcatc gagcagtaat agtttcgggc gtttggcaag gcttcgggcc agggccacac
     9481 gctgtcgctg accaccggaa agctgatgcg gtttgcgttt ggcgaactcc tgcatgtgca
     9541 ccaggccgag catctcatgg acccgactgg caatttccgc tttcggtagt ttgtcctgtt
     9601 tcaggccaaa agcgatgttc tgttccacgg tcatatgcgg aaacagcgcg taagactgaa
     9661 acatcatatt gatggggcgc aggtaaggcg gcacctgtga caaatcgacg ccatcgagca
     9721 ttatctgccc ggcagaaggt tgttcaaaac ctgccagcat acgcagcagc gtggacttgc
     9781 cacagccgga tgcgcccagc agcgcgaaga tttcaccttt gtagatggtc aggctgacat
     9841 catcgaccgc atgctgacca tcgtaggatt tggtgaggtt gcggatttct aatagcggcg
     9901 tcagcgcctt acgggttttc gcctgcgggc gagggatagc gtcattcacg gctgctctcc
     9961 ggcattgaat gtgtgcaagc gcaccatgat ggtgcgaatt gccggctggt gtggtgcgcc
    10021 cgcccggcat ctaaggctgc ggattatttt ccgctcttca ctttggtcca cgcgcgggtg
    10081 cgcacacggt cgattttcgg atcctgtact ttcagagtga atagcttcgc acgaacgtcc
    10141 gccggaggat aaatacccgg gttctcacgg acttccgcac tcaccagcgg cgtggctgct
    10201 ttattggcgt tggcatagaa cacatggtcg gaaatatgcg ctactacatc cgggcgcagc
    10261 aggtaattca ggaactgata ggcttcgtct ttgtttttgg catccgcagg catggcgaat
    10321 acatcaaaga acgccatcgc cccttctttt ggaatcgaga acgagacatt cactccattc
    10381 ttcgcttcct tcgcgcggtt tgacgcctgc cagacatcac ctgcccagcc gatagcgacg
    10441 caaatatcgc cgtttgccag gtcgttaatg tattgagatg aatggaaata acgaatgttc
    10501 gggcgcagct ttaacagcag atcggttgcc ggtccggtgt aatcatccgc tttagtgctg
    10561 ttgggatctt tgccgagata attcaacacg gtagcaaaaa cttcttctgg cgcatccagg
    10621 aaagagacac cgcagctttt cagtttttcc agattttcag gtttgaggat caagtcccag
    10681 ctatcgacgg gcgcgttttc gcccagcaca gctttaactt tatcaacgtt atagccaatc
    10741 ccggtcgtcg cccacatata gggcatagca aatttattgt cgggatcgtg tttggcgacc
    10801 agcttcagca gctccgggtc gagattcttc cactccggca atttgctttt gtccagcggc
    10861 tggaaaactc ccgcagtcaa ctggcgttca agaaagctgg cagatggaac caccaagtca
    10921 aagccggtgc tacccgccat taatttgcct tccagtactt cgttagagtc gaaaacatcg
    10981 tagacgactt taataccggt ttctttttca aaattggcca ccgtgtccgg ggcgatataa
    11041 tcagaccagt tataaatgtg gagtgttttt tgttcagccg cgagcgtgcc gacagagacg
    11101 gccatcagag cacccgcagc cagacccgaa agccattttt tatttaaggc ggtcatatct
    11161 ctttccttct gaaagttcgt taacaaacga acaaaagttg tcacactaaa agatatgcaa
    11221 taaacgtgca tattttttat cggcggggtg taagaaaaga gaactctctc ccggccagca
    11281 ctgctcgcta tataaaagca tcgcaagaat aactttagcc agggtttgag actatagctc
    11341 ggattaaaaa acggtattta acaaattttt atgcgtattt gctctatgtg ataaagccaa
    11401 atcgacacaa gagaagtgaa ataatcttta agaaaaggtt aaaaacagca gataattttg
    11461 cagcgcgcag ccactgcacc agcgactgcg tgctcgaaag gagttatcag tggaggaaat
    11521 agttttcggt aacgttgttt tgcccttcat cgtcagtcgg taacagcagt tgatgggcat
    11581 tagcttcaag aatgaccatc gaaatctgct cttcgctctg acgcacgaac catgcaaact
    11641 gctcataggt tacgccctgc ataacggata aagactggca aaccaccagc tttggcagat
    11701 tatcatcctg catatcaaga aatgctttca cggtcagcga actggcattg atggcagata
    11761 aatccgccgc cagcggcaat acggctgaag gtcggacttc cgccatggca gaaaacagga
    11821 tcgtgttatc gatcagatcg attttggcat caaagacacc gtcgaaattc tgcatatggg
    11881 gcagatgcag agcctgacag ttatcacatt caaaaaaact catccccagg tcattgagcc
    11941 attgacgcag cgtatccaga ccaggaacga ccagcgatgt cataattttg tacaacctct
    12001 ttcgataaaa agaccggcac agcttacgca aaaagcgcag gcaaaaccat gatcagtaat
    12061 gtgattgcga ctaaccaccc gttttcaggc aatattctgt cgtagcgtgg cgttcgatcc
    12121 agcggatcat tttacccgcg atgtcgatac cagtggtttt ttctattcct tccagccccg
    12181 gcgacgcatt cacttccatc accaacggcc cgcgattagc acgcagaata tccacaccag
    12241 caacgtccag cgccatcgtt cgcgcggctt ttatcgcgat ttcacgctcc tgtggtgtga
    12301 tactggcgac gcttgccgcg ccgccacgat gcaaattgga acgaaaatcg ccctctttcg
    12361 cccgccgttc aatcgcagcg accacttcat cgccaacaac cagacagcgg atatcgcacc
    12421 cttgcgcctc tttgatatat tcctgcacca gaatatgcgc gttcagaccg cggaaagcgt
    12481 caatcacgct ttccgccgcc tgacgcgtct ccgccagcac gacgccaatt ccctgcgtgc
    12541 cttcaaccaa cttgaccacc agcggcgcac caccgaccat gtcgattaaa tcgctggtat
    12601 catccggcga atgcgcaatg cccgtgacag gcaggtcgat gccctgacgc gccagcagtt
    12661 gcatggaacg caatttgtca cgcgcccggg caatggcgac cgactcattg agcggatagc
    12721 tccccagcat ctcgaactgg cgcagtgccg ccgtcccata aaaggtaatg gcggtgccaa
    12781 tacgcgggat cactgcgtca aaatggggta acttgcggcc tttgtagtga atagaagacg
    12841 ccgcaggatt tatgttcatg tagcaagaaa gcggatcaag aatttcaacc aggtgaccgc
    12901 gctgtatagc ggcttcacgc agccgcttac acgaatagag cgttccatcc cgggacaata
    12961 tggcaatttt caccctgcac ctctctgtca gacctggtca aaagcccgcg tatcatacat
    13021 cgacgtggcg gttttagcgc gtcgcccaac cctgtttgtg caaataatcc agaataaatg
    13081 ggcggctttc tttaatgatt gttcggcgga tatgatcgct ccaggtatcc cggcgattat
    13141 tgctgccacg ggtgaggtaa tattccgcca gttgctcgtc atactgcgcc agtgcgtctt
    13201 tatccagcgg ttgatagctg ttttcatgca ccaaaatgga ggccggtaaa cgcggcttga
    13261 gatccggatt atccgcaggc cagccaaggc acagcccaaa cagcggcaga acatgctgcg
    13321 gtaatttcag cagtttcgtc accgcttcaa tattattgcg caggccgccg atatataccc
    13381 cgcccaatcc cagcgattcc gctgcggtta atgcattctg cgccatcatt gccgtatcaa
    13441 cgacaccgag caacagttgt tccgccaggc cgagctgagc atccggacag atctgtaaat
    13501 ggcggttaaa gtcggcacag aacacccaga actccgccgc ttgcgctacg tgtttttgcc
    13561 cgccggtcag cgtcaccagt tcttcacgta atgctttgtc ggtaatgcga ataatgctac
    13621 tacactgcaa aaaactggaa ctggacgtcg cacgggcgct gttaataatc gcctcacact
    13681 gcgcttcgga aatgggttca tcagtgaaat ggcgaatgga gcgatggcca caaataagtt
    13741 caatggttgg cgtcattatc tttttctctt tctgaacgtg aatattgcgg tggacggttc
    13801 atcagctgtg gggcaagacg ttttgccacc tgaagaataa ccaccaccgc agcgggaagc
    13861 atgagcaaaa caccgagaaa aatcatcaga atctgcactt ctggccgaga aaatggctca
    13921 ggcagcgaca gggagtcgct taccgacagc agcgccaccg ccagtagcat cattccgata
    13981 aattccagta tcaacacgcc tttaggcaat ttaccgatcg cgcgcatacg cttccctctg
    14041 caaagtgagc cttcagtcta aaacttttca ctgtattgtg tttaacagtt atagctttta
    14101 gcaattaatg caacaggtta aacctacttt cagcgaatac attttagcgt gatcattaca
    14161 ggcataaatc tatgaggaga gaaataatgc aaaccgttat ttttggtcgt tcgggttgcc
    14221 cttactgtgt gcgtgcaaaa gatctggctg agaaattgag caatgaacgc gatgattttc
    14281 agtatcagta tgtagatatt cgtgcggaag ggatcactaa agaagatcta caacaaaagg
    14341 caggtaaacc cgtagaaacc gtgccgcaga tttttgtcga tcagcaacat atcggcggct
    14401 ataccgattt tgctgcatgg gtgaaagaaa atctggacgc ctgatcgtct gacaagccct
    14461 cgcgttgagg gctttactga ttttttctgt gctgtggttt aaacaaacta ctgataaata
    14521 agaaacacag tgcccccagc gcacaccaga acaccgcgct tagtaaccat gccagctctt
    14581 gccagaatga gcgcgtcggt gaaaaaaaca gccgcataat gagcattgaa cagggtgccg
    14641 ccagcattgc gccaaataga ggtttcagga cttctctacg ctgtgaaaag aaactggcaa
    14701 ctgctccagg aagaatgaaa aacagcaagc cgatttcagg atgcccggca gcccgaaaag
    14761 cgcctttcat gtgcgtcgcc agaaaaaggc acaccacaat gaagaggaca aaacagcaga
    14821 ttgcccccgc ccaacgttgt ttatgtttca ctcgttcctc ctgacactgc gtctatcgaa
    14881 cacatttttc gccagtgtgg cgttcagtaa gataaagccg cttcgcattc catgctaata
    14941 taggccaacg caattcatat agccgttgat acctaatgtg attacactag taaaatatat
    15001 tgttacttta ctatcgttta ggtgcgctga atgaatctgc gccctgaatt ctggtaaaaa
    15061 acattatcgt aaattaccat ttctttcaac agcttactag taaacaagaa gttagcctcc
    15121 gtgaatataa acgtcgccga attgttaaat gggaattaca ttctgttatt atttgtggtc
    15181 ctcgcgcttg ggctatgtct cggaaagtta cgacttggtt cgatccaact gggtaattcc
    15241 attggcgttt tagtcgtatc gctgttatta ggccaacaac atttcagcat taacaccgat
    15301 gcgcttaatc ttggctttat gctgtttatt ttctgcgtcg gggtcgaagc cggaccgaac
    15361 tttttttcca ttttttttcg cgatgggaaa aattacctaa tgttagcact ggtgatggtt
    15421 ggcagtgcgc tggtgatcgc cttagggtta ggtaagctgt ttggctggga tattggcctg
    15481 acggccggta tgttagcagg ctctatgacg tcgacaccgg ttctggtcgg tgctggcgat
    15541 acactgcgtc attccggcat ggaaagcagg cagctctcac tggcactgga taatctgagc
    15601 ctcgggtatg ccttaaccta tttaatcggt ctggtgagtt tgattgttgg tgcgcgttac
    15661 ttgccgaaat tgcagcatca ggacttacag accagcgccc agcaaatcgc ccgcgaacgt
    15721 ggcctggaca ctgatgccaa ccgtaaggtt tatttaccgg tgatccgcgc ctatcgcgtc
    15781 ggcccggagc tggtggcctg gaccgacggc aaaaatctgc gtgaactggg tatttatcga
    15841 caaaccggct gctacattga acgtattcga cgtaacggga ttctggcaaa tccagacggt
    15901 gatgccgtgc tacaaatggg cgatgaaata gcgttggtag gctatcccga cgcccatgcc
    15961 cgactcgatc ccagcttccg taacggtaaa gaagttttcg atcgtgacct tctcgacatg
    16021 cgtatcgtca ctgaagaagt ggtcgttaaa aaccataacg ctgtaggtaa acgtctcgca
    16081 caactgaagt tgaccgatca cggttgcttc cttaaccgcg tcattcgtag ccagattgag
    16141 atgccgatag atgacaacgt cgtgcttaac aaaggtgacg ttttacaagt cagcggtgat
    16201 gcccgtcgcg taaaaaccat cgccgatcgc atcggcttta tctcgattca cagccaggtc
    16261 actgacctgc tggcattttg cgccttcttt gttattgggc tgatgatcgg gatgatcacc
    16321 ttccagttca gcacattcag tttcggcatg gggaacgctg ccgggttgtt attcgccgga
    16381 attatgctgg gctttatgcg tgctaaccac ccgaccttcg gttacattcc gcaaggtgca
    16441 ttaagcatgg tgaaagagtt cggcttgatg gtgtttatgg caggcgttgg tctgagcgcc
    16501 ggtagcggta ttaataacgg cctgggcgcg attggcggtc agatgttgat tgccggatta
    16561 attgtcagtc ttgtgcccgt ggttatctgt ttcttgttcg gtgcttatgt attgcgaatg
    16621 aaccgcgcac tgttgttcgg cgcaatgatg ggcgcacgca cctgcgcgcc ggcaatggag
    16681 atcatcagtg atacagctcg cagtaacatc cctgcgctgg gctatgcggg cacctacgca
    16741 atcgccaacg tcctgctgac gctggcaggg acaatcatcg tcatggtatg gccaggatta
    16801 ggataaaact gaagttgccc tgaaaatgaa atttttttgc acaaccgcag aacttttccg
    16861 cagggcatca gtcttaatta gtgccactgc ttttctttga tgtccccatt ttgtggagcc
    16921 catcaacccc gccatttcgg ttcaaggttg atgggttttt tgttatctaa aatttatcta
    16981 ctaccctgca accctctcaa ccatcctcaa aatctcctcg cgcgatagcg gcttacggtc
    17041 ggtgacaaaa tgcagcgtca tcccctcaat aaacgcatca agcgcgcggg cggttccggg
    17101 ttcaaaccat tgttcgagcg tttgctgact gcgctgcatc cagttttgca ttaccgtttt
    17161 taatagcggt tttcggctag ccagcgcgta gagctggtac atcagctcca tattatccgg
    17221 cgttgcaacc tgtgagctgt agatcatatc ggtgatagcc tggcatgcgc cttgagcatc
    17281 actaacatcg ctaaaaaatg cctgatattg ccgggacata atctcagtaa aactgctgaa
    17341 cgcctccagc aacaactcat caattcctga aaaatagtag gtcatcgatc ccaacggtac
    17401 cccggcaagg gtagcaattt tgcggtgcgt aacagcatgt attccgtaaa gtttcaccgc
    17461 ctccagcgtg gcctggataa ttttttctcg ccgttgcgga tcgttagcgc gacgcatgtc
    17521 atttcctctg tctgcaattg tgtacaaatg tacacaacct tgctaatgtt gtcttccctc
    17581 ttcttatttt gacgtgatct atgaccgtaa attcttcacg taatgcattg aaacgccgaa
    17641 cctgggcgct gtttatgttc ttctttttgc caggcctgtt aatggcgtcc tgggcaaccc
    17701 gtacgcctgc tatccgcgat attctctctg tctcgatcgc tgaaatgggc ggtgttctct
    17761 ttggtctgtc gatcggttcg atgagcggta ttctctgctc ggcgtggtta gtgaaacgct
    17821 ttgggacacg taatgtcatc ctggtcacga tgtcctgcgc attgatcggg atgatgatat
    17881 taagtctggc actctggctg acatcgcccc tgctctttgc cgttggtctc ggcgtctttg
    17941 gggcaagttt tggttctgcg gaagtggcga taaacgttga aggtgccgcc gttgagcgag
    18001 aaatgaataa aacggttttg ccgatgatgc acggttttta tagcctaggc acgctggcag
    18061 gcgctggtgt cgggatggca ctgacggcct ttggcgttcc ggcaacggtg cacattttat
    18121 tggcggcgct ggtaggtatc gcgcctattt atatcgccat tcaggcaatc cctgacggta
    18181 cgggcaaaaa tgctgccgat ggcacccagc atggcgaaaa aggcattcct ttttatcgcg
    18241 atatccagtt gctgttgatt ggtgttgtag tgctggcgat ggcctttgcc gaaggttctg
    18301 ccaacgactg gttaccctta ttaatggttg acggtcacgg ttttagccct acttccggct
    18361 cgctgattta tgccggtttt accctaggga tgaccgttgg acgcttcact ggcggttggt
    18421 tcatcgaccg ttacagtcgc gttgccgtgg ttcgggccag tgcactaatg ggggcgttgg
    18481 gtattgggct gattattttt gtcgatagcg cctgggtcgc tggggtgtct gttgtactct
    18541 ggggactggg tgcctcgctg ggcttcccgc tgaccatttc tgccgccagc gataccggcc
    18601 ccgatgcacc aacccgcgtc agtgtggtag caacgaccgg ttatctggct ttcctcgtcg
    18661 ggccgccgct gctgggctat ctcggcgaac attatggatt acgtagtgca atgctggttg
    18721 tactggcgct ggttattctc gcggctattg tcgcgaaagc cgtcgccaaa cccgatacca
    18781 aaacgcggac ggcgatggag aatagttgat gagcattaaa ttaattgcgg tagacatgga
    18841 tggtactttc ttaagcgatc aaaaaaccta taaccgtgag cggtttatgg ctcagtatca
    18901 gcaaatgaaa gcacaaggca ttcgctttgt ggtcgccagc gggaatcaat attatcagtt
    18961 gctctctttc ttccctgaaa ttgctaatga aattgccttt gtggctgaaa acggcggctg
    19021 ggtagtgagt gaaggcaaag atgtttttaa tggcgagctg tcgaaggatg cctttactac
    19081 tgtcgtggaa catttgctga cgcgcccgga agtggaaatt attgcctgcg gaaaaaatag
    19141 cgcctatacg ctcaaaaaat atgacgatgc catgaaaacg gtggcagaaa tgtattatca
    19201 ccgtctggaa tacgtcgata actttgacaa cttagaagat attttcttta agtttggcct
    19261 gaatctttcc gatgaactga ttccacaagt acaaaaagca ttacatgagg ccatcggcga
    19321 tattatggtg ccagtccaca ccggcaacgg cagcatcgat ctgattatcc ccggcgtaca
    19381 taaagccaat ggccttcgcc aactgcagaa attatgggga atagacgaca gcgaagtggt
    19441 ggtctttggc gatggcggta acgatattga gatgctgcgt caggcaggct ttagttttgc
    19501 aatggaaaat gccggcagcg cggtcgtcgc agcggcaaaa taccgggcag gctccaataa
    19561 ccgtgaaggc gtactggatg tgatcgataa agtgcttaaa cacgaagcgc catttgacca
    19621 ataaaaacgg cagcacgccc ggcaacacaa tacctgaatg aaggctgcat aattcgcgga
    19681 aattaacccg ctgcattatt tcaggcgaaa tgattatgaa aaattgtcta ctgttgggcg
    19741 cgcttttaat gggctttact ggtgtggcga tggcgcaaag tgtcaccggc gatgtgccga
    19801 gtggatacaa agtggttgta gttcctgatt cagtcagcgt tccacaagcg gtcagcgtcg
    19861 cgactgtgcc gcaaaccgtc tatgtggctc ctgcgccagc tccggcttac cgcccgcatc
    19921 cttatgctcg ccatcttgcc agcgtaggtg aagggatggt tatcgaacac cagattgacg
    19981 accatcacca ttaattcgag aatgcctgat cgcacaaagc agtcaggcat ttttttaccc
    20041 ttcgtgagaa tttcccatct gtttatcttt taaaaagata accatcagcg acagccacaa
    20101 aattccgttg acaaggttga agagattaaa ctgtccgttg cccccgttca gccaggcatg
    20161 tttgctgatt tcaataccaa cggtaaagat cagcatttgc agcattccca tcgccgcaga
    20221 aaccgtacct ttactcatat cgctggcaaa cagggttaat cgcaccagtc ccgcattcgc
    20281 cagaccaata ccgaaagcat aaatacttaa cccggcagtc atccataaat acgcgtgcga
    20341 tgagataacc gttgccgcag cagcgaccaa cagaccaatc ataatcggcc agccgcccat
    20401 aattatcacg tacggtgcgg cgcgaggtca gacgcgctaa cagcaagtta cccgcaatta
    20461 acgccccgaa aataggcact tgcagcaagc catattcata gctgctcaac tgctccccgg
    20521 taatgatgat aatcggcgac tgggcgatcc acgccagcaa tggcaggcta acgaatccca
    20581 gcgccagcgc ccccgccaca aagcggccgt tcttcagcac cagcttatag tcacgaccga
    20641 gttctttcag tgacagtttc tcgcctatac gcgtggcggt ttcaggcata gctcgttgca
    20701 gaccgaaaaa ggagatcgct gccaatgcgg caaacaaaac aaacatcccc tcccagggca
    20761 gcacatggat ccacgccgcg cccaccagcg gaccaagtag cggagcaatc agcgccacgt
    20821 tcgccatcag cgcggtgatc ttgatacaaa ccgcctcttc gaaggattcc tgaattgcgg
    20881 cgtatcccac agcgccaatg aaacagaggc ttatgccctg caagaagcgc aacagggtga
    20941 attgttcaat gttttgcgcc agtaatattg ccagacaggt gacgataaac cacaccacac
    21001 ccgccagcat caccggacgg cgaccaatac gatccgacag cggccccagg agccattgta
    21061 aaaacatccc gcccgccaga tacgcggtca tcgaagtagg aacccaatca atgcccgcct
    21121 gatattgttc caccacggcc aacataccgg gttggatcat atcgttgccg atataggttg
    21181 aaaattcgta aagcaccaga cagagaggga aaagtaacgc ctgacgtcca agcctggcac
    21241 cgaaagctaa tttattttgc atgcaatttc ttcgccaata ataatcgcgc agagtttaat
    21301 aaaagcgcag ctaacgagaa agcgaatttt gtagctgaaa ccacggttaa gcacattctt
    21361 acattattgc gagtatagct acgctttctt taagttttat ttaacctatg cccgttacaa
    21421 tcacccaccg taaacaggcc gcttgaggga aataagacga tgccgcttta cccagtttaa
    21481 cctgcacttt attctcaacg acttgcctgt attggctccc ttttaatcac tttgcgtcgg
    21541 gaagttatgc tggaaaattt gaatctctct ctattctctc ttattaacgc gacgccagac
    21601 tcggctccgt ggatgatctc gttggcgatt tttattgcta aagatttgat taccgtggtg
    21661 ccgttgctgg ccgcggtact ttggttgtgg gggcttacag cacaacggca actggtgata
    21721 aaaatcgcta tcgcgctggc ggtcagcctg tttgtgtcct ggacgatggg acatcttttt
    21781 ccgcacgacc gaccctttgt cgaaaatatc ggctataact tcctgcatca tgcggcggat
    21841 gactcattcc cgagcgatca cggtacggtg attttcacct ttgcactggc atttttatgc
    21901 tggcatcgcc tgtggtccgg ctcactttta atggttctgg ccgtcgtcat tgcctggtcg
    21961 cgcgtttatc ttggcgtcca ctggccgctg gatatgctcg gtggattgct ggcaggtatg
    22021 attggctgcc ttagtgccca gattatctgg caagcgatgg ggcataaact ctatcaacgt
    22081 ctgcaatcgt ggtatcgcgt ctgttttgca ttaccgatcc gcaaaggctg ggtgcgtgac
    22141 tgatctctgc taaaaagtgt agtattgagc ggctcgcttc aataactatt cagagggatt
    22201 atggaaacac gtcgcgaaga gcgtatcggg cagctgctgc aagaattaaa acgcagcgat
    22261 aagttacatc ttaaagacgc cgccgccctg cttggggttt cggagatgac gattcgtcgc
    22321 gatctgaaca accacagtgc gcccgtcgtt ttgctcggcg gctatattgt tctggaaccg
    22381 cgcagtgcca gccattacct gttaagcgat caaaaatccc gcctggtgga agaaaaacgc
    22441 cgggcggcaa aactggctgc gacactggta gaacccgatc agaccctctt ttttgactgt
    22501 ggcaccacca cgccgtggat tattgaagcg attgataatg aaatcccttt taccgccgtt
    22561 tgttattcgc taaatacctt tctggcgctg aaagagaaac cccattgccg cgcatttctg
    22621 tgcggtggtg aatttcacgc cagcaacgcc attttcaacc ccatcgattt tcagcaaacg
    22681 ttgaataatt tttgcccgga tatcgctttt tattccgcgg cgggcgtgca tgtcagtaaa
    22741 ggcgctacct gttttaatct tgaagagttg ccggtaaaac actgggccat gtcgatggcg
    22801 caaaagcatg tgctggttgt cgaccacagt aaatttggca aggtgcgtcc ggcgcgcatg
    22861 ggtgacctga aacgctttga tattgtggtg agtgattgtt gcccggaaga tgagtatgtg
    22921 aagtacgcgc agacgcaccg tattaagttg atgtattaat gacgtataac cggatgacgt
    22981 ttcgcgccat ccggttatca gaagattaag agaaccagct gccgaaccac tgatggaatt
    23041 tcatcatcac gaaatcccac atccgaccaa agaatccgcc ctcttccaca ttttccatca
    23101 cgatcagcgg acgctgctca atggatttac cgttaagctg gaaatcaatg gtcccgacaa
    23161 cctgaccttt tttcagcggt gcggtaagct gcggttccgt taacgtgtag ctcgccttca
    23221 ggtttttcag ctgcccacgc ggtatggtca cggagcccgc ttcgcctgcc ccgagattca
    23281 cttcgctctt atcaccaaac cagacgcgct gagtcacaaa ggtggcatca ggtttaattg
    23341 gcgtcacagt ttcaaagaag cggaaacccc aggtcaataa tttctcagac tcattaaaac
    23401 ggatacggtc agttttcgcc cccagcacta cggagattaa acgcatatca ccctgggtag
    23461 ccgaagcaac cagattatat cccgcacctg ccgtggttcc tgtcttcatg ccatcaacat
    23521 tcagattgct gctccacagc agacggttac ggttaggctg acgaattttg ttgaaggtga
    23581 actctttctc tttatgaatg gcgtactctt ccggcacatc gtggatcaat gctttaccca
    23641 gcaatgccat atcgcgcgcg gtactgaact gccccggcgc atccagaccg tgcaccgtct
    23701 ggaaggtagt gttggtcaga cccagttttt tggcataacc attcatcaaa ccaataaatg
    23761 actcctggct cccggcaacg taatcggcca gcgcaataca ggcgtcatta ccggactgga
    23821 taatcacacc tttgttcaag tctgccaccg aaacctgatc gcccggtttg aggaacatta
    23881 ccgatgaacc acgcagtgcc ggatttcccg tcgcccaggc atctttaccg accgtcacca
    23941 tatcggtgag tttaatctta tcggccttaa gcgcctgccc aaccacatag ctggtcatga
    24001 ttttagtcag gctcgcggga tccagtttct catccgcgtt gccttctgcc agcactttac
    24061 cgctggcgta atccattaaa atccatgcac gcgcatccac gctcggcgct tcaacggttt
    24121 gttccgccgc gaatgccgtt ggggcaaaaa ggaataaaaa tgcagaaccc gctgcaagac
    24181 cacgaaggag agaggagtat tgcgtcataa atgccacccg agtatccatt caaaaaaaat
    24241 acgttatatc gccgtatgat aagttcggtg attaataacg cacacacaaa cttaaagaaa
    24301 ccgtaagttg gtaaagtttt taaagtttat gcaatatcct gccgcttcgc tgcttctggt
    24361 gtcatttttt tgccagctgt tgcttaaaag ttaactttac ttcaccatcg gagtcctgtt
    24421 cagcctgtgg ctgcctgtcg gaaaaggggt gagtatgatt acgctgtggg gtcggaataa
    24481 ttcaactaac gtaaaaaagt attgctgacg ctcgaagaac tggaactacc ttatgagcaa
    24541 attctcgcgg gccgtgagtt tgggataaat cacgatgctg attttctggc gatgaaccct
    24601 aacgggctgg tgccgttgtt gcgtgacgac gaaagtgatc tcattctttg ggaatcaaac
    24661 gccattgtcc gctatctggc ggcacagtac gggcaaaaac gcctgtggat cgactcaccg
    24721 gcacgtcgtg cggaagcgga aaaatggatg gactgggcaa accagacgct cagtaatgct
    24781 catcgcggga tcctgatggg attagtcaga acaccaccgg aagagcgcga tcaggccgcc
    24841 attgatgcca gttgcaaaga gtgcgacgcc ctgtttgccc tgctcgatgc ggaactggca
    24901 aaagtaaaat ggttctctgg cgacgagttt ggtgtgggcg atatcgctat cgcaccgttc
    24961 atctacaatt tgtttaacgt tggcctgacc tggacaccgc gtccaaatct gcaacgctgg
    25021 tatcagcaac tcactgaacg ccccgcagtg cgcaaagtgg tgatgattcc cgttagctaa
    25081 ttgcgtgggc taactttaag taattcccca ctggactcgt cggtgagaac gtataaataa
    25141 ccgtcgggtc cagtgcgaac atcacgaatt cgctgccctc tgtccgttaa aatacggcca
    25201 tcttctgtca ctttgtcgcc gttgacgctc atcacaatga catctttatc tttcagcgcg
    25261 ccaataaata atttttgctg ccactggggg aatttatcgc tgttatagaa ggccatgccg
    25321 ctcacagcgg gcgaatcttt ccagtaaaaa acaggttgct cggtcccggc gacgatctcc
    25381 cctttcgctt ccggtatctt aaagcctgaa tagttgattc cccaggttgc cagcggccag
    25441 ccgtagtttt tgcctttttg cgggatatta atttcatcac caccgcgcgg gccatgttca
    25501 ttcagccaca gtgcattact ccacggattc atcgccattc cttgcggatt acgaatgcca
    25561 taagaccaga tctcggcgcg cgcaccggat tcctttataa aaggattatc atccgggatt
    25621 tcgccctggt cggtcagacg caccagtttg ccctgtaatt tatccagatc ctgcgccgtc
    25681 gggcgctgat tgttttcgcc cagagcaata aaaagataac ctttaccgtc gaataccagc
    25741 cgcccgccaa aatggttgcc ggtagacagt tttggcatct ggcggaagac ggtgcggaag
    25801 tcggtcactt ttgagagatc atcacttaag cggccataac ccacagcagt tccggctttg
    25861 ccatcatcgc caacttcgga ataacttaac cagatgcggc gagactgagc aaaatcaggc
    25921 gctaaaacca cgtccagcag gccgccctgc ccgtgcgccc aaacgtccgg aactccggaa
    25981 agcggcgcag ataatccttt tcctgcttgc cagtgacgca actcgccgcc gcgcagagtg
    26041 attaacatac cgtgattatc gggtaaaaag gccagtgccc agggatggtc gagtttgtct
    26101 tgcagtactt cgacatttac cgttgcagga gccgcccaga gagcggaaga aagacaaata
    26161 aggggcacaa ggaaaaagga ttgtcgatgc ataacgcctc cggattgact ctggcttaaa
    26221 gcgtagtcag tggaggagat aacaaattca tttttacaaa aacttaaaca tgaaggggga
    26281 gacgctttct cccccttagt tttcaggcct tctcaagcat ggcgtgcttc tgcaggctct
    26341 ggatactcag cgttaagctc atcagacaat tttcaagctt atcggcgttg acggtaataa
    26401 cagtcgggca atcatggtgc ccactcatca aacatactgc ggctgtcgct aatgcttctt
    26461 cagcatgatg aagagcactc cactcttcct gatccagatg aagattcaac cgcagcgatt
    26521 tatcgtgcag ttcgcgattc agtttaaaaa agttatctcg tagatgattg ctttcgctga
    26581 cggacatgta tccttttgcc ttcctcagtt gcacgtatgc agcgaggtca acgcgtgcgt
    26641 cgatcaaccg gttcagcaga tcgattcgct gtctgtcaac gaacatactt cgatcctcct
    26701 ctttccagcc ttgttgcgat taaatagtat ggctattttt tgagcaaaat gctgtccagg
    26761 atcaattctt cacgccaaat taacaaaatt tacatttcct tcgcctccgc cagcgaatga
    26821 gattgcgata caaattttat ccagaactct gtagaatccc tgccctgacc tttcctgtaa
    26881 ctgaatattt tttaagctat gagcaaagta actccccagc cgaaaatcgg ctttgtttcc
    26941 cttggctgtc cgaaaaacct tgtcgattca gagcgtattc tcaccgaact acgcactgaa
    27001 ggttatgacg tggtaccgag ctatgacgat gcggacatgg tgatcgtcaa cacctgcggc
    27061 tttattgaca gcgcggtaca agaatcactg gaagccattg gtgaagcgtt gaatgaaaac
    27121 ggcaaggtaa ttgtgaccgg ttgtctgggg gcaaaagaag atcagatccg cgaagtccac
    27181 ccgaaagtgc tggaaatcac cgggcctcat agctatgagc aggttctgga gcacgttcat
    27241 cactacgtgc caaaaccgaa acacaaccca ttcctgagcc tggtgccaga acaaggtgtg
    27301 aaactgacac cgcgtcatta tgcctatctg aaaatttcgg aaggctgtaa tcaccgctgc
    27361 accttctgca ttattccgtc tatgcgcggc gacctggtga gccgtccgat tggcgaagtg
    27421 ttaagtgaag cgaaacgtct ggtagatgcg ggcgttaaag agattctggt gatctcgcag
    27481 gatacttccg cctatggcgt tgatgttaaa catcgtactg gcttccacaa cggcgagccg
    27541 gtaaaaacca gcatggtcag cctgtgcgaa cagttatcga aactggggat ctggacacgt
    27601 ctgcactacg tttaccctta tccgcatgtg gacgacgtca tcccactgat ggcagaaggc
    27661 aaaatcctgc cgtatctgga cattccgttg cagcacgcca gcccgcgcat tctcaaactg
    27721 atgaagcgtc cgggttctgt agatcgccaa ctggcgcgca tcaaacagtg gcgcgaaatc
    27781 tgcccggaac tgaccctacg ctcaaccttt attgtcggct tccctagcga gacggaagaa
    27841 gatttccaga tgctactcga cttcctgaaa gaagcgcgtc tggatcgcgt tggctgcttt
    27901 aaatacagcc cggttgaagg tgcagacgcc aatgccctgc ctgaccaggt tccggaagaa
    27961 gtgaaagaag aacgctggaa ccgtttcatg cagctgcagc agcagatttc cgccgagcgc
    28021 ctgcaagaga aagtgggccg tgaaattctg gtgattatcg acgaagtgga cgaagaaggc
    28081 gcgattggtc gcagcatggc agatgcaccg gaaatcgacg gcgcggttta tctcaatggt
    28141 gaaaccaacg ttaagccggg tgatatcctg cgtgtgaaag tcgagcacgc cgatgagtac
    28201 gatttgtggg gtagccgggt ttaattaatt caggccgggc aaaatcattt gtccgccctg
    28261 cgaataatga ttactgccag ctaatctgag ccctgccgtt tttcttactc ttatacagcc
    28321 gctcatccgc cagcattttc aagcgttcga cggtggtaca ttcatttgaa tgcgctacac
    28381 cgatactggt acttaaatta atgcttccca cctggaaatg aacgatttct tttagaatcg
    28441 atagcaactc ttccagcgtt ccacccgtat aaatcaccgc aaattcgtca ccgccgaagc
    28501 ggtagatttt acctttatct accgggatct gtgatttaag gattttcacc actttcatca
    28561 acacttcatc gcccagcaaa tgaccgaagg tgtcgttaat gtttttgaat ttatcgacgt
    28621 caaacaggca caaataaccg cctttttccg gcagcgcttt taaatcttcg tcaaaggatt
    28681 ttctgcccat tgctttggtc aggtcgtcca ggaacgccga gttatacata ctgtagagtt
    28741 ttttgacatc ggcagccgaa aagaaaagcc cgccatccag tacgcgttca aaatggttat
    28801 taatgatggc ttctttctcc acaataccac gattaatcac tctgcgtacc agatagaggc
    28861 aactggtacc cgatatcact gccaccaatg cggcaaagaa aataatcaat gtacagaccc
    28921 gcaaataaac atctttttta tacgcaaata cgtacaatcc ggtcggcaaa taggcgaagt
    28981 gctccatcgc ttccgaaacg atgaagatgg cgtttttagc attatattca tcaccggttt
    29041 accgttctta aatagagaga acatcaggtt ctcgctgtcc atcgccagcg attttgacag
    29101 taaagaaaaa cgctccagac caagcaaaaa tatcagacta cgattgtgag ttaccggata
    29161 gaggtaatag acgaaggttt taccgtcaaa agagataaac cctgtatttt tgacatgtcg
    29221 atacttcaca acctgcataa tatctgcaca ataatttttc tcgaactcct ttacctgaat
    29281 acattcgtca ctggaaatca gataaggacc gacatcgtac aaataagagg gggaaaccga
    29341 gtcggcgtaa gctgtgcgca aaccaaccaa cagctgttcc tccgtgggat ttagcgtgtt
    29401 gataagatca attgccacgc tacgccgata ttcagcaata tttgccaccg caaaagcagt
    29461 atcaataacg gactgtttca ggctatacac ccgttccttt accatttgag tgtagatccc
    29521 gcaggcaact gctgaaatca tgataaaaat cagcagactg actgtaagta cgaacttatt
    29581 gattctggac atacgtaaac tactctttta ctaattttcc acttttatcc caggcggaga
    29641 atggcatggg tttctggtac agatacccct gagcctgaac acatcctaat ttaattaatt
    29701 cgcgcgcaac gtcttcgctc tctacgcctt ccgcaatcac cgggatccgg ttatagcgtg
    29761 ccagatcgaa aataatgcgc acaatttttc ccgcagtacc gaaaatatcg ccggtaaatt
    29821 gcgagtccac ctttatgctg tctggattaa tttcataaaa acgtttcagg tttgacaaac
    29881 cggtgccaaa gtcatcaatc gatatccgta ccccttgttt acgcagttgt tcaatattga
    29941 gataaaaatg ttcattaatt cgctcgatca cggtttcagt gatctcaaca cagaggctgt
    30001 tcgccttcag gccattttct ttcatgatgg tggtaaaacg ctcatgaaag tgcggttcgt
    30061 ttaactgcca gggcgaaaca ttaatatgca gtaagaaatc atccttcagc gacgcacgct
    30121 gactaaaatg tctgaattca cccattgccg ttttaataat ctgataaccc agctcattga
    30181 taaacccgat atcttcagca atggtaatga acttcagcgg tgaaatgatc ccccgctccg
    30241 cagaaaccca acggcacaac gcctccgcgc ccagaatggc gcgggtgtta atatcaacga
    30301 tcggttgata gacgaggaat agttcgccaa tttccattgc atggcgaata ttacgcgcaa
    30361 tccagatatt ctcttctatt tcgttgagtt tcgcgtcatc acagaaaata aactcggaga
    30421 acatatggtc acggatactg gaaatcgcca gtttcagttt ttctgcgtaa ctccaggcgt
    30481 tttccccctg caaaggttca caaatggcag acttatggat gcgcagattg cggtagccaa
    30541 agctgctgtc aaaatcgttt accatcgcta ttttacgggt aaatgtctgt acattttccc
    30601 gacacagaag taaatagaga tcggcattga atgtcacgat atcgcagcat tgcgcatatt
    30661 tctcggcaat tcgctgacac aatttcctga cgattttatt gcgttcatca acaccaaaca
    30721 ccgcttccac ggcttccaga ccacacagtt tgatgatccc cacagccaga tactgattat
    30781 tgttggactc aataatcgcc atatttttaa taaaaccgct ttcctgatcg taagagatca
    30841 gtgagagcaa tttatttgac cgttcaattt cggcatcttt catttccgcg atcgaa
//
LOCUS       DAZPLI010000034        30078 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZPLI010000034 DAZPLI010000000
VERSION     DAZPLI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30078
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.034"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHUU3_001507"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHUU3_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR2081611.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHUU3_001508"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHUU3_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR2081612.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHUU3_001509"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHUU3_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR2081613.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHUU3_001510"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHUU3_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR2081614.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHUU3_001511"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHUU3_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR2081615.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHUU3_001512"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHUU3_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR2081616.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHUU3_001513"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHUU3_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR2081617.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHUU3_001514"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHUU3_001514"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR2081618.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHUU3_001515"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHUU3_001515"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR2081619.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHUU3_001516"
     CDS             complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHUU3_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR2081620.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHUU3_001517"
     CDS             complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHUU3_001517"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR2081621.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHUU3_001518"
     CDS             complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHUU3_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR2081622.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHUU3_001519"
     CDS             complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHUU3_001519"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR2081623.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHUU3_001520"
     CDS             complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHUU3_001520"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR2081624.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHUU3_001521"
     CDS             complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHUU3_001521"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR2081625.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18363..18463)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18516..18992)
                     /locus_tag="ACHUU3_001522"
     CDS             complement(18516..18992)
                     /locus_tag="ACHUU3_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rsd/AlgQ family anti-sigma factor"
                     /protein_id="HFR2081626.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKLGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19087..19860
                     /gene="nudC"
                     /locus_tag="ACHUU3_001523"
     CDS             19087..19860
                     /gene="nudC"
                     /locus_tag="ACHUU3_001523"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR2081627.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19900..20964
                     /gene="hemE"
                     /locus_tag="ACHUU3_001524"
     CDS             19900..20964
                     /gene="hemE"
                     /locus_tag="ACHUU3_001524"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR2081628.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20974..21645
                     /gene="nfi"
                     /locus_tag="ACHUU3_001525"
     CDS             20974..21645
                     /gene="nfi"
                     /locus_tag="ACHUU3_001525"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR2081629.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21688..22278
                     /locus_tag="ACHUU3_001526"
     CDS             21688..22278
                     /locus_tag="ACHUU3_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR2081630.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22465..22737
                     /gene="hupA"
                     /locus_tag="ACHUU3_001527"
     CDS             22465..22737
                     /gene="hupA"
                     /locus_tag="ACHUU3_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR2081631.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22750..23445
                     /locus_tag="ACHUU3_001528"
     CDS             22750..23445
                     /locus_tag="ACHUU3_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR2081632.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHUU3_001529"
     CDS             complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHUU3_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR2081633.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24110..25486
                     /gene="zraS"
                     /locus_tag="ACHUU3_001530"
     CDS             24110..25486
                     /gene="zraS"
                     /locus_tag="ACHUU3_001530"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR2081634.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25483..26808
                     /gene="zraR"
                     /locus_tag="ACHUU3_001531"
     CDS             25483..26808
                     /gene="zraR"
                     /locus_tag="ACHUU3_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR2081635.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHUU3_001532"
     CDS             complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHUU3_001532"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR2081636.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHUU3_001533"
     CDS             complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHUU3_001533"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR2081637.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7030 a         7852 c         8288 g         6908 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctacgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac atataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgct tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggtaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaat ccctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttggtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggagctg gtgccggaac gcctgcgtgg tgaaactgca
     5101 tctttcgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ttggctaagc tgagccagtc tggtcacaag
     5341 cgtattgaaa cgctgttcac caacgacctg gatcacggcc cgtatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacctgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ctgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggac
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc cgggtggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcatt acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctt tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaggtga ccgacggtgt tgtaactgac gaaattcact acctttctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgtga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtttg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtgtatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccactggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 ccagaccatt actcgtcaga ccgacgaact gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gctcgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatgggtg ttattcagag ggcaaattct tcacggaaaa tattaagaaa ggcagggcga
    12781 aacgaggaag aagcttttcg ccctggacaa cattcctgct gacggcacta ccataaaaaa
    12841 acacaacgct ttcaacaacc ttgcgttttt catcttccgt agcggctcgg aataaattta
    12901 cgttcttaca ttccgtctca tagtctttgc gaggcgcgtc ccagatagct gtcccagtct
    12961 ttccataccg gctgcaaacc ctgagcggtt aacgcggcag caaccgcttc cggtctgcga
    13021 tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat cggcatagcc acctggctgc
    13081 gttttcgaga aggcgctgac gttattgatc gccagcggaa taacgcgatc gcgaaaccac
    13141 ggtgattccc gcgtggagag tgacagttca atctccggtg caagcagtcg gaaggcgcag
    13201 atggtttgca ctaactggcg ttcatccata atcgacgcag gctcaatgcc gccagtacac
    13261 gggcgcaggc gcggaaagga gacagagtaa cggctttgcc agtaatgctg ttgcagccat
    13321 agcaaatgtt cggcaaccat atagcagtca acgcgccagt tgtcggaaag gccaattagc
    13381 gcgccgaggc ctatcttatc aatccccgca cgccccagcc gatccggcgt ttccagccgc
    13441 cagaagaagt cctgtttttt gcctttcaaa tgatggcggg tataagtcgc ctcgtgatat
    13501 gtctcctgat aaaccatcac cccatccaga ccaagttgct ttaactcggc gtattccgtc
    13561 tccgccagcg gttgcacttc catttgtagt gaagagaact gttcacgcag ggcggggaga
    13621 tgacgacgaa agtaatccat ccccactttc gcctgatgtt caccagtgac taatagcaga
    13681 tgttcaaagc ccatctcccg tatagcggcg ctttccctgg caatatccgc ttcatccagc
    13741 gttttgcgct tgatgcgatt actcatggaa aatccgcagt acgtgcagtc gttagcgcaa
    13801 agattggaaa gataaagcgg gacgtagaaa ctaactgtgt tgccaaatcg ctgacgggtc
    13861 agacgctgcg cccgttgggc cagtggttcc agatagccac tggcggctgg cgataacagc
    13921 gccatcatat cgtcgcgggt gagttgcgag gcatttagcg cccgttctac gtcagcagcc
    13981 gttttgccgt tgatacgcag gcggatgtcg tcccagtcca gttgtcgcca gcgatcgctg
    14041 aaggttttca tgccgatgcc tccagaaatc cagtcagcgg gctggtggca tgagcaaaat
    14101 gactgcggct gcccggtccg gactgacgtg ccagcaggcc tgcttctacc gccagacgaa
    14161 atgccttcgc catgttgacg ggatcgtccg ctacggcaat cgccgtattc actaacaccg
    14221 cgtcggcccc catttccagg gcctgcgcgg catggctggg aacgccgatg ccagcatcga
    14281 caaccaccgg aactgtggcc tgctggataa taatctccag catggcacgg gtttccagtc
    14341 cctgattcga gccaatcggc gcgccgagcg gcatcaccgc tgcacagccg acttcttcca
    14401 gacgtttaca caataccgga tcggccccgc agtaaggcag cacgacaaat ccctgttgta
    14461 ccagcatttc ggcggctttc agagtttcga tgggatcggg caacagccag cgggcgtcag
    14521 ggtgaatctc taattttaac cagtttgtgc ctaacgcttc acgtgccaga tgggcggcga
    14581 aaatggcttc ttccgctgtt ttcgccccgg aagtatttgg cagcagggtc acacccgccg
    14641 cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa gtcgacacgt ttcatcgcca
    14701 gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac catcagttgt gaagaagcga
    14761 atttccctgt gccggtaaac agatgtgaat caaacgtttt gtccgcaata cgtaacatct
    14821 caaccccctg caataacctg aaaaagcagg atctggtcgc catcctgcac gatatgttgc
    14881 gtccactgct cacgcgggac gatttgctga ttaatcgcca gagccgcgcc cgcttgtcgt
    14941 tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg cggcgcactg catcggttga
    15001 tcgttaaaca ggatctgcat tgcttcctcc gcataccggg caaccactgg cgcggcgcaa
    15061 cgccaggctg cgccactggc tcgatttacc gtcgaacagt cggagttctc ccgcaggtgt
    15121 ctctataccg cttaataact taatggcttc cagtgcctgc aaagtgccca taaccccgac
    15181 caccgggcca accacgcccg ccgtgcggca gttgcgttct ggctcctggt tatctggcca
    15241 caggcagcgg taacacccct gctcccaggg cggcgtcagt accatcaact gaccgccaaa
    15301 tccgaccgcg ctggcggtga taagcggcgt gttgagtacc acgcaggcgg cattaatctc
    15361 ctggcgagtc gccatattgt cggtacagtc gagcaccaca tcggcccgtg caaccgcatc
    15421 ttttaacgcc tcacccgtta accgttgttg taatgctgtc agttgaatat cgggattcaa
    15481 ctgtgtcagt cgctgatggc tgacctgcga tttcgggcga tcgatatctt cagtggtaaa
    15541 gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg tcatctgcca gtaccagcgt
    15601 cccgacgcca gcccccgcca ggtatagcgc agcaggtgta cccagcccgc caagaccaat
    15661 aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc agagcgatat cgtcgagcag
    15721 aatttggcgg ctataacgca taaagtcacg gtcattcatc gccaactcct gcaatttcca
    15781 gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt aatggcgctg acgacggcga
    15841 tactgccgac acccgttgct atcaccgcag gcgcgcgcgc cagactgata ccgccaatcg
    15901 ccacggtggg ataatccgcc agtcgctcaa catgccgtgc cagctgttcc agcccctgcg
    15961 gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg tcccagcgcg atataagagg
    16021 ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg tgtcgaaacg cccagccgca
    16081 ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa atcttcctgc cccaaatgga
    16141 cgccatacgc ctgatgcttg atcgccagcc gccagtaatc gttgataaac aatcgcgcgt
    16201 tatagcggcg gcccagcgca attgccgcca cgacatcggc ttccacctct tcattgcgcc
    16261 gatctttgat gcgtagctgg agagtacgta cgcctgcatc caacagacgt tcgatccact
    16321 gtacgctgtc caccaccggg tacagtccta aacgaaaagg tacaggagga aaatcaggct
    16381 gatacatcac gcttcctcct tacgcaggta gatttctccg cctctggcgc ggaagttctc
    16441 cgacatatcc gccattccca cttcaatggt ttgcgcggcg gcgtaatcac gtacttcctg
    16501 gctgattttc atcgagcaga atttcggccc acacatggag caaaaatggg cgactttacc
    16561 tgactcttgc ggcagggttt catcgtgata agcgcgggcg gtaaacgggt cgagggccag
    16621 attaaactgg tcttcccagc gaaattcgaa gcgggctttc gacatggcgt tatcgcgaat
    16681 ttgcgcgcct ggatgccctt tcgccaggtc agcggcgtgg gcggcaatct tataggtgat
    16741 aagcccctgc ttaacatctt ctttattagg cagacccaga tgctcttttg gcgttacgta
    16801 acagagcatc gcgcagccaa accagccaat catcgccgca ccaatccccg acgtgaagtg
    16861 gtcatagccc ggcgcaatat cggtagttag cggccccaga gtgtaaaacg gcgcttcgtg
    16921 gcagtgctct aactcctcgg tcatattgcg gcggatcatc tgcatcggca cgtggcctgg
    16981 gccttcaatc atcacctgca cgtcatattc ccaggcaatt ttggtcagtt cgcccagcgt
    17041 atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga atagaaccgg ggcgcagacc
    17101 gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt tcacaaattt cgcggaagtg
    17161 ttgatagagg aaattttcct gatgatggga gaggcaccat ttcgccataa tcgaaccgcc
    17221 gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc acatagcgca gcagtacgcc
    17281 cgcatggata gtgaagtaat ccacaccttg ctcggcctgt tccagcagcg tgtcgcggaa
    17341 cgcttcccag gtaagatctt cggcgatccc gttaaccttc tccagcgcct ggtagatcgg
    17401 cactgtaccg atcggcaccg ggctgttacg caaaatccac tcgcgggttt cgtgaatata
    17461 gcgaccggtg gagagatcca tcaccgtatc cgctccccag cgcgtggacc ataccagctt
    17521 ttccacttct tcttcgatgg aagaggtgac cgccgagttg ccgatattgg cgttaacttt
    17581 taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc ggatgattaa tgttggccgg
    17641 gataatcgca cgtccggcag caacttcatc acggacaaat tccgcagtga tattttccgg
    17701 cagatgtgcg ccaaagctca ttcccggatg ctggtggcgt aaaacctcgc tacggatgcg
    17761 ctcgcggccc atattctcgc ggatggcgat gaattccatt tccggcgtga tgatgccctg
    17821 gcgggcgtag tgcagttggg tgacacggcg tcctgttttg gcgcgttttg gcgttaataa
    17881 gccgctaaaa cgtagctcgt cgagaccatc atctgccagc cgcgttctgg tgtaatcgga
    17941 actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg atccacggct ggcgtagttt
    18001 tgccagccct tgctgcacgt taatggcgat ctgcggatca ccatacgggc cggaggtgtc
    18061 gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt tctttgctac cgccaattag
    18121 cgtcgggcta agctggatct cacgcatcgg cacgcgcacg ccggggtgtg tgccagtgat
    18181 ataaatgcgt tttgagttgg gaaaggcggt gccttccagg gtgtcgataa aatgttgggc
    18241 ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac atagctcatt ccaaaaagtc
    18301 aaggacgtgg cttgtcagac gacggatgaa gcaagagacg atcgccgcaa aggcgatgcg
    18361 atagcagatt aactcttgtt cccttcgcag gtattagcct gatcaggttc cgcggatccc
    18421 gaataaacgg tctcagccag ttaaggcact ccgacaagaa attagccccc acaaacgggg
    18481 cgttgaatgt aaattacgcg ttaacagcgc agaactcaag caggatgttt gacgcgggcg
    18541 gcgtcaagca ccagcagaat cagcttatct tccagcacaa agcgcgcttc cagcgcctcg
    18601 ccgatgtcag ataaaacctg ttgaaactca aggtaattat catgatcgat ggcggtttcc
    18661 aggctggaat cgtagtaatc cataatctgt tgggtattgg cttcgagttg cggccaaatc
    18721 ttcgcggcgc gcgcgagttg cccgttgcct tccagcttat gaagaatgcg ctcataaata
    18781 ctgaaatgtc cggcagacaa gtaatcgacc aggctctgac aaaaatcatc aagggctttt
    18841 tcgtttagcc tcatgtacga ttctttgcca agcttaatgc caaccagatt gtagtaagcc
    18901 acgagcagat gcttacgtac atgtagccag cgatcaacca gtttgttact tcctctgacg
    18961 cgctccgtca ggttatcgag ctggttaagc atgattgact ccgcaagttt gtattcaaaa
    19021 actgctcagt gagaaatgta aaaaccatgt taaacatgcc agtgatgcaa aggtagtgca
    19081 agagctatgg atcgtataat tgaaaaatta gatcacggct ggtgggtcgt cagccatgaa
    19141 caaaaattat ggttgccgaa gggagaattg ccatatggcg aagcggcaaa tttcgatctt
    19201 gtgggtcagc gcgcactaca aatcggcgaa tggcaggggg aacctgtttg gttagtacaa
    19261 cagcagcggc gtcacgatat ggggtcggta cgtcaggtca ttgatctcga tgttgggctg
    19321 tttcaactgg ccggacgagg cgtacaactg gcggagtttt accgatcgca taaatactgt
    19381 ggttactgcg ggcatgaaat gtatccgagc aaaaccgaat gggcgatgct gtgcagccat
    19441 tgccgtgagc gttactaccc gcaaatcgcc ccctgcatta ttgttgccat ccgtcgcgat
    19501 gattcgatcc tcctcgccca gcatacccgc catcgtaacg gtgtccatac agtacttgcc
    19561 ggattcgtcg aagtgggcga aaccctcgag caggcagtcg cgcgggaagt gatggaagag
    19621 agcggaatta aagttaaaaa cttgcgttac gtgacttctc agccgtggcc gtttcctcag
    19681 tctttaatga ccgcgtttat ggcggaatat gacagcggcg acatcgtgat cgacccgaaa
    19741 gaattgctcg aggcgaactg gtatcgctat gacgatttgc cgttactccc gccgcccggc
    19801 accgtagcgc gccgtctgat agaagatact gtggcgatgt gtcgggcaga gtatgagtga
    19861 tgatacactg accgcctgac gcactaagga acagccaaaa tgaccgaact taaaaacgat
    19921 cgttatctgc gggcgctgct gcgccagccc gttgatgtca ctccagtatg gatgatgcgc
    19981 caggcgggtc gctatctacc ggaatataaa gccacgcgcg cccaggcggg cgattttatg
    20041 tcgctgtgca aaaacgccga gctggcgtgc gaagtgactt tgcaaccgct gcgtcgctac
    20101 ccgctggatg cggcgatcct cttttccgat atcctcaccg tgccggacgc gatggggtta
    20161 gggctctatt ttgaagccgg agaaggtccg cgttttacct cgccagtcac ctgcaaagcc
    20221 gacgtcgata aactgccaat tccggacccg gaagatgagc tgggttacgt gatgaacgcg
    20281 gtgcgtacca ttcgtcgcga actgaaaggc gaagtgccgc tgattggttt ttccggcagc
    20341 ccgtggacgc tggcgaccta catggtggaa ggcggcagca gcaaagcgtt caccgtgatc
    20401 aaaaaaatga tgtatgccga tccgcaggcg ctgcacgctc tactcgataa actggcgaaa
    20461 agcgtcactt tgtatctgaa tgcgcagatt aaagccggtg ctcaggcagt gatgattttc
    20521 gacacctggg gcggtgtgct taccgggcgc gattatcaac agttctcgct ctattacatg
    20581 cataaaattg ttgatggttt actgcgtgaa aacgacggtc gccgcgtgcc agtcacgctg
    20641 tttaccaaag gcggcggaca gtggctggaa gcgatggcag aaaccggttg cgatgcgttg
    20701 ggcctcgact ggacaacgga tatcgccgat gcgcgccgcc gtgtgggcaa taaagtcgcg
    20761 ttgcagggta atatggatcc gtcgatgctg tacgcgccgc ctgcccgcat tgaagaagaa
    20821 gtagcgacta tacttgcagg tttcggtcac ggcgaaggtc atgtctttaa ccttggtcac
    20881 ggcattcatc aggatgtgcc gccagaacat gctggcgtgt tcgtggaggc agtgcatcga
    20941 ctgtctgaac aatatcaccg ctaaggagtg attatggatc tcgcgtcatt acgcgctcaa
    21001 caaatcgaac tggcttcttc tgtgatccgc gaggatcgac tcgataaaga tccaccggat
    21061 ctgatcgccg gagccgatgt cgggtttgag cagggcggag aagtgacgcg agcggcgatg
    21121 gtgctgctga aatatccctc gcttgagctg gtggagtata aagttgcccg catcgccacc
    21181 accatgcctt acattccagg ttttctttcc ttccgcgaat atcctgcgct gctggcagcg
    21241 tgggagatgc tgtcgcaaaa gccggattta gtgtttgtcg atggtcatgg gatctcgcat
    21301 cctcgccgtc ttggcgtcgc cagccatttt ggtttgatgg tggatgtgcc aacgatcggc
    21361 gtggcgaaaa aacggctctg cggtaaattc gaaccgctct ccagcgaacc gggcgcgctg
    21421 gccccactga tggataaagg cgagcagctg gcctgggtct ggcgcagcaa agcgcgctgt
    21481 aacccgttgt ttatcgctac cggccatcgg gtcagcgtgg acagcgcgct ggcgtgggta
    21541 caacgctgca tgaaaggcta tcgtctgccg gagccaacgc gctgggcgga cgcggtggcc
    21601 tcggaacgtc cggcgttcgt gcgctataca gcaaatcagc cctaattcag gtaaactgcg
    21661 gataatttcc gtatttgaga actcaacatg ttacaaaacc caattcatct gcgtctggag
    21721 cgcctggaaa gctggcagca tgtcactttc atggcttgct tatgcgaacg catgtacccc
    21781 aattacgcca tgttctgcca gcaaaccggt tttggtgatg ggcaaattta ccgtcgtatt
    21841 ctcgatctca tctgggaaac gctgaccgtt aaagacgcaa aagtaaattt cgacagccaa
    21901 ctggagaaat ttgaagaagc gattccttca gccgacgatt tcgatctgta cggcgtttat
    21961 ccggcaatcg atgcctgcgt ggcgttaagt gaactggtcc attcgcgttt gagtggtgaa
    22021 acgctcgaac acgcggtgga agtgagtaag acctccatca cgaccgttgc gatgctggaa
    22081 atgactcagg ctggtcgcga aatgagcgat gaagagctta aagaaaaccc agctgtagag
    22141 caagaatggg acattcagtg ggaaatattc cgacttttag ctgagtgcga agaacgcgat
    22201 atcgagctga taaaaggcct tagggcagac ctgcgtgagg cgggtgagag caatattggt
    22261 ataatttttc agcaataaga ccagaaaacg tgatttaacg cctgatttgt cgtacctgga
    22321 gtcttccctt tcgccccccg tctggtctac atttgggggg cgaaaaaaag tggctatcgg
    22381 tgcgtgtatg caggagagtg ctattctggc atttccgtcg cactcgatgc ttagcaagcg
    22441 ataaacacat tgtaaggata acttatgaac aagactcaac tgattgatgt aattgcagag
    22501 aaagcagaac tgtccaaaac ccaggctaaa gctgctctgg agtccactct ggctgcaatt
    22561 actgagtctc tgaaagaagg cgatgctgta caactggttg gtttcggtac cttcaaagtg
    22621 aaccaccgcg ctgagcgtac tggccgcaac ccgcagaccg gtaaagaaat caaaattgcc
    22681 gcagctaacg taccggcatt tgtttctggc aaggcactga aagacgcagt taagtaagat
    22741 tgcgtggcag tgaacagttt taacgaaggg gtggtttcac cccttttgtc tttctggcgt
    22801 cgatcattga tgctggctgg cgctctgctt ctcactgcct gtagtcataa ctcttcactt
    22861 cctcccttta ccgccagtgg atttgctgaa gaccagggcg cggtacgcat ctggcgaaaa
    22921 gacagcggcg ataatgtgca tctgcttgcc gtgtttagcc cgtggcgcag tggcgatacc
    22981 acgacgcgag agtatcgctg gcagggcgat aacctcacgc tcatcaatat caatgtttac
    23041 agcaaaccgc cggtgaatat tcgcgcgcgt tttgacgatc gcggtgatct gagctttatg
    23101 caacgtgaat ccgatgggga aaagcagcag ctttctaacg accaaatcga tttataccgt
    23161 tatcgtgctg atcagatccg ccagattagc gatgccttac gtcaggggag agtcgtgctg
    23221 cgccaggggc gctggcatgc gatggaacag accgtgacca cctgcgaagg gcaaaccatt
    23281 aaacctgatt tagattcgca ggcgatagcg catatcgagc gccgccagag ccgctcttct
    23341 gttgatgtca gcgtggcatg gctggaagcg cccgaaggtt cgcaattact gttagtggca
    23401 aactctgatt tctgtcgctg gcaacccaac gagaaaacgt tctgatttac cagtggccca
    23461 tacccatatg accgccacca ccgcagccgc cgtagcccat gcccattccg gctccgcgcg
    23521 gaatacccgc ttcagccatc gcgatatctc gtttcacccg taactcatct aacgactgac
    23581 gcaaattctc catctctttg gcgaccgcgt taattttgct gctatccggt gggttcgcgg
    23641 ctaacagggc attgtattca taacgcttcg tcaccagttg ctgttgcagt gcgctgcttt
    23701 gagcgtaaaa gtcattatgg attttctgcc acgctgtctg ttgttcgctg gtcaaaggcg
    23761 cggcattttg ctgccacata ccgtgtccgc cgtgagcaaa tgcagatgtc gatcccatcg
    23821 ccattgctga aagcgccatc attaccaggg caattttcgt gttccgtttc atggttaatc
    23881 ctccagtggt tgtcttactt cgggtattgc atcttccgtg ccaacgatga aacgctgata
    23941 tgacgggtaa tctggcatga taaacgagta aaaatgactc gcctgctgcg ggtagcgagt
    24001 catttttact cattgaaact taagcctttg tgttacagcg cagggtaagc gctgataaaa
    24061 gatggcatga tttctgctgt cagaaaggga tgagcaggca aagaagaaga tgcgttttat
    24121 gcaacgttct aaagactcct tagctaaatg gttaagcgcg atcctccccg tggtcattgt
    24181 tgggctggta gggttgtttg cggtgacggt gattcgcgat tatgggcgcg agactgccgc
    24241 cgccagacaa acgctgctgg aaaaaggcag tgtacttatt cgcgctcttg aatccggctc
    24301 gcgcgtcggc atggggatgc gcatgcatca tgcgcagcag caggccttac tggaagaaat
    24361 ggccgggcag cctggagtac gttggtttgc ggtcacggat gaacaaggaa caatcgtgat
    24421 gcatagcaac tccggcatgg tgggaaaaca gctttattcc ccgcaggaaa tgcagcagtt
    24481 acatccggga gatgaagaag cgtggcggcg gatcgatagc gcagacggcg agcctgttct
    24541 ggaaatttat cgccagtttc aaccgatgtt tgctgctgga atgcaccgga tgcgccatat
    24601 gcagcagtat gccgcgacac cacaagcaat tttcatcgct ttcgacgcca gtaatattgt
    24661 gagtgccgaa gatcgtgagc agagaaacac cctgattatc ctcttcgccc tggcgacggt
    24721 cttgctggca agcgtattgt cattcttctg gtatcgccgc tatttgcgct cgcgccagct
    24781 tctacaagat gaaatgaagc gcaaagagaa gctggtggcg ctggggcatc ttgcggcagg
    24841 cgttgcccac gaaatccgta acccactttc ctcgattaaa ggactggcga aatactttgc
    24901 cgagcgcgcg cctgcagggg gagaagcgca tcaactggcg caggtgatgg cgaaagaggc
    24961 cgaccgttta aaccgcgtgg taagcgagct gctggaactg gttaagccaa cgcatctggc
    25021 tttgcaggcg gtggatctca acacgctgat taaccactca ttacagctgg taagccagga
    25081 tgcgaacagc cgggagatcc agttacgctt taccgccaac gacacattac cggaaattca
    25141 ggccgatccg gacaggctga ctcaggtcct gttgaatctc tatctcaatg ctattcaggc
    25201 gattggtcag catggcgtga ttagcgtgac ggccagcgaa agcggcgcgg gcgtgaaaat
    25261 cagcgttacc gacagcggta agggaattgc ggcagatcag cttgatgcca tcttcactcc
    25321 gtacttcacc actaaagccg aaggcaccgg actggggctg gcggtcgtgc ataatattgt
    25381 tgaacaacac ggtggtacaa ttcaggtcgc aagccaggag ggaaaaggcg caacgttcac
    25441 cctctggctt ccggtcacta ttacgcgtaa gggcccacaa ggatgacgca cgataatatc
    25501 gatattctgg tggtggatga tgacattagc cactgcacta ttttgcaggc tttactgcgc
    25561 ggctggggct ataacgtcgc gctggcgaac agcgggcgac aggcgctgga gcaggtgcgg
    25621 gaacaggttt ttgatcttgt gctttgcgat gtgcgaatgg cggagatgga cggcattgcc
    25681 acgctaaaag agatcaaagc gttaaatccg gcgattccgg tgctgattat gaccgcatac
    25741 tccagcgtcg agacggcggt agaagcgttg aaaaccgggg cgctggatta tctcatcaag
    25801 ccgctggatt tcgataacct gcaggcgacg ctggaaaagg cgctcgcgca tacgcacagt
    25861 attgacgctg aaacgcctgc ggtgtctgcc agccagttcg gtatggtcgg taaaagcccg
    25921 gcgatgcaac acctgctcag tgaaatcgcc ctcgtcgcgc catcggaagc cacggtgctg
    25981 atccacggcg attccggcac cggtaaagag ctggtcgcca gggcgattca cgccagtagc
    26041 gcacgtagcg aaaaaccgct ggtaacgctc aactgtgcgg cactcaacga atccttgctg
    26101 gaatctgaat tgttcggtca cgaaaaaggg gcgttcactg gagccgatag acgccgggaa
    26161 gggcgctttg ttgaggcgga cggcggcacg ctgtttctcg atgaaattgg cgatatctcg
    26221 ccgatgatgc aggtgcgtct gctgcgtgcg attcaggagc gcgaagttca gcgtgtcggt
    26281 agcaaccaga ctatctcggt tgatgtccgg ttgattgcgg cgacccatcg cgatcttgcc
    26341 gcagaggtga atgccgggcg ttttcgccag gatctctact atcgcctgaa tgtggtggcg
    26401 attgaagtac catcgctgcg gcaacggcgg gaagatatcc ctctgctggc tgtccatttt
    26461 cttcagcgct ttgccgagcg taatcgcaag gcggtaaaag gctttacgcc acaggcgatg
    26521 gatttgctga tccactacga ctggccggga aatattcgtg aactggaaaa cgcggtggaa
    26581 cgggcggtgg tgctgctgac cggggaatat atttccgaac gcgagctgcc gctggcgatt
    26641 gccggtacgc cgatcccact ggggcaaagt caggatattc agccgctggt ggaagtcgaa
    26701 aaagaagtga ttctggcggc actggagaaa acgggcggca acaaaaccga agccgcccgt
    26761 cagttaggga tcacgcgcaa aacgctattg gcaaaactgt cgcgttagtt ctgctcgcgt
    26821 tcgatagcgc gccagccgat atctttccgg cagaagcagt cgtcccagtg aatatcggtc
    26881 attaaggcat aagcgcgttt ctgcgcttct gccacggtat gacccagcgc ggtgacgcac
    26941 agtacgcgcc cgccgctggt cactacctgc tcgttatccg ccagtttcgt gcccgcgtgg
    27001 aacactttgc cgcctgccac ttcttccagc ggcaggccgt ggatcacatc gccggtgcgg
    27061 taatcgcccg gatatccacc cgcagccatc accacgccga gagaagcacg ctcatcccac
    27121 tctgacgttt tctcgtccag tttgccttca caggccgcca ggcagagctc aaccagatcg
    27181 gacttcatgc gcagcataat cggctgggtt tctggatcgc caaagcggca gttaaattcg
    27241 ataaccttcg gattgccctg tttgtcgatc atcaggcccg cgtagagaaa accggtgtag
    27301 gtgttgcctt ccgccgccat gcctttcacg gttggccaga tgatacgttc catggtgcgc
    27361 tgatgaactt catcggttac taccggcgcg ggggagtaag cgcccatccc gcccgtgttt
    27421 ggtccggtat ctttatcgcc tacgcgtttg tgatcctggc tggtggccat cggcagcaca
    27481 tgctcgccgt ctaccatcac gataaagctc gcttcttcgc cgtcgaggaa ctcttcgata
    27541 acgatgcgat gacccgcgtc gccaaaagcg ttgcccgcca gcatatcgtg aacagccgct
    27601 tcggcttctt ccagcgtcat cgccacgata acgcctttcc cggctgccag accgtccgct
    27661 ttaatgacga tcggcgcacc tttctcacgc agatacgcca gcgcaggttc tacctcggtg
    27721 aagttctggt attccgctgt agggatctta tggcgggcca ggaaatcttt ggtaaacgct
    27781 ttcgaacctt ccagttgcgc ggcacctgcg gttgggccga agattttcat cccggcggcg
    27841 cggaaggtat cgaccacgcc tttcaccagc ggcgcttccg ggccgacgat ggtcagatca
    27901 atcttttcgt tttgtgcgaa atccaacagc gccgggatat cggtcacgcc gatagcaacg
    27961 ttttgcagcg cgggttccag cgcagtgcct gcattacccg gagcaacaaa aacagtttca
    28021 accagcggcg actgcgccgc tttccaggcc agcgcgtgct cgcgcccgcc gttaccaatc
    28081 actaatactt tcatctattg ctccattaat ggcggaagtg gcgcatgtcg gtgaagagca
    28141 tcgcaatacc gtgctcgtcg gcggcggcaa tcacttcgtc atcacggata gaaccgccag
    28201 gctggattac gcaggtcacg cctgcagcgg cggcggcatc aataccgtcg cggaacggga
    28261 agaacgcgtc agaagccatc gaggaacctt tcacttccag gccttcatcg gcagctttaa
    28321 taccggcgat tttcgcggag tacacgcggc tcatctggcc cgcgccaatc ccgatggtca
    28381 tattgttttt ggcatagacg atagcgttgg atttcacgaa cttcgccact ttccagcaga
    28441 acagcgcatc acgcagttcc tgttcggtcg gctgacgttg ggtgacgacg cgcagttctt
    28501 ccgcaccgac catgcccagg tcacgatcct gaaccagcag accgccgttc acgcgtttga
    28561 aatcaagacc cggaacacgc tcgccccact gaccgcaggt cagaacgcgt acgttctgct
    28621 tggcggcggt gattttcagg gcttcttcgc tggcggaagg cgcaataatc acttcgacaa
    28681 actgacgaga aatgatggcc tgtgcggttt ccgcatccag ctcgcggtta aaggcaatga
    28741 tgccgccgaa tgcggaggtt gggtcggttt tgtacgcgcg atcgtaagca tcaagaatgg
    28801 aattgccgat agccacgccg caagggttgg cgtgcttcac aatcacacat gccggctcgg
    28861 cgaactcttt cacgcactcc agcgccgcat cggtatcggc gatgttgtta taagagaggg
    28921 ctttaccctg aacctgggtt gcggtagcaa cggaggcttc tttcacattc tcttctatat
    28981 agaaggcagc ctgctggtgg ctgttctcgc cgtaacgcat atcctgcttc ttaatgaagt
    29041 tcaggttcag cgtgcgtggg aagcgaccgg cggcttcttt gctttcaccg tggtaagccg
    29101 gaaccatgct gccgaagtag ttggcaatca tgctgtcgta ggcggcagtg tgttcgaagg
    29161 ctttgatggc gaggtcgaaa cgggttgcaa gcgtcagcga tccttcgttg tcatccatct
    29221 ctttaataat ggcgtcgtag tcgctgctct tcaccacgat tgcgacatct ttatggttct
    29281 tagcggcgga gcgcaccatg gtcgggccgc cgatatcgat gttctcaacc gcatcttcca
    29341 gcgagcagcc ttcacgagca acagtctggg cgaacggata caggttaaca accaccatat
    29401 cgataggctg gatctgatgt tcttccataa tggcatcgtc ctggccgcga cggcccagaa
    29461 tgccaccatg tactttcgga tgcagggtct tcacgcgtcc atccatcatc tccgggaaac
    29521 cggtgtaatc ggaaacttcg gttaccggca gacctttttc tgctaacaga cgggcagtgc
    29581 cccctgtaga cagcagctcc acaccgcgtg cggaaagtgc ctgggcgaat tcgacgatac
    29641 cggctttgtc agaaacactg agcagagcgc ggcggactgg acgacgttgt tgcatggtaa
    29701 atcccctgga tttgactatt acagagagcg ttagctgaat ttttcgcgaa aaactcagct
    29761 aacgccccta acggggcatc cttatttttc gcccgcattg taacgaaaac gtttgcgcaa
    29821 cgctcgcgaa tttttctctt tcaatggtga tcacaatttt gacagtggtt accgtgggaa
    29881 aaatacagaa attacattga tgattgtgga taactctgtg cgtaaaaagg tataaagcgg
    29941 ggttttgctg gggaatgcag cagtcagtca tttttctgca atttttctat tgcggcctgc
    30001 ggagaactcc ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac
    30061 agccggttcg gttgaaga
//
LOCUS       DAZPLI010000035        29548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZPLI010000035 DAZPLI010000000
VERSION     DAZPLI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.035"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="ACHUU3_001534"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="ACHUU3_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dGTP triphosphohydrolase"
                     /protein_id="HFR2081638.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="ACHUU3_001535"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="ACHUU3_001535"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR2081639.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHUU3_001536"
     CDS             2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHUU3_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFR2081640.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3644..4030)
                     /locus_tag="ACHUU3_001537"
     CDS             complement(3644..4030)
                     /locus_tag="ACHUU3_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR2081641.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHUU3_001538"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHUU3_001538"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFR2081642.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHUU3_001539"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHUU3_001539"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR2081643.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHUU3_001540"
     CDS             complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHUU3_001540"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR2081644.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHUU3_001541"
     CDS             complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHUU3_001541"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR2081645.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHUU3_001542"
     CDS             9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHUU3_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR2081646.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10793..11644
                     /gene="tsf"
                     /locus_tag="ACHUU3_001543"
     CDS             10793..11644
                     /gene="tsf"
                     /locus_tag="ACHUU3_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR2081647.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHUU3_001544"
     CDS             11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHUU3_001544"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR2081648.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12808..13365
                     /gene="frr"
                     /locus_tag="ACHUU3_001545"
     CDS             12808..13365
                     /gene="frr"
                     /locus_tag="ACHUU3_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR2081649.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13457..14653
                     /gene="ispC"
                     /locus_tag="ACHUU3_001546"
     CDS             13457..14653
                     /gene="ispC"
                     /locus_tag="ACHUU3_001546"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR2081650.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14842..15600
                     /gene="ispU"
                     /locus_tag="ACHUU3_001547"
                     /gene_synonym="uppS"
     CDS             14842..15600
                     /gene="ispU"
                     /locus_tag="ACHUU3_001547"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR2081651.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHUU3_001548"
     CDS             15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHUU3_001548"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR2081652.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16482..17834
                     /gene="rseP"
                     /locus_tag="ACHUU3_001549"
     CDS             16482..17834
                     /gene="rseP"
                     /locus_tag="ACHUU3_001549"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR2081653.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17864..20296
                     /gene="bamA"
                     /locus_tag="ACHUU3_001550"
     CDS             17864..20296
                     /gene="bamA"
                     /locus_tag="ACHUU3_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR2081654.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20418..20903
                     /gene="skp"
                     /locus_tag="ACHUU3_001551"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20418..20903
                     /gene="skp"
                     /locus_tag="ACHUU3_001551"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR2081655.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHUU3_001552"
     CDS             20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHUU3_001552"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR2081656.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHUU3_001553"
     CDS             22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHUU3_001553"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR2081657.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHUU3_001554"
     CDS             22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHUU3_001554"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR2081658.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHUU3_001555"
     CDS             23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHUU3_001555"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR2081659.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHUU3_001556"
     CDS             24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHUU3_001556"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR2081660.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHUU3_001557"
     CDS             25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHUU3_001557"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR2081661.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28557..29516
                     /gene="accA"
                     /locus_tag="ACHUU3_001558"
     CDS             28557..29516
                     /gene="accA"
                     /locus_tag="ACHUU3_001558"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR2081662.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7160 a         7121 c         8160 g         7107 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
     6541 ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat
     6601 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
     6661 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
     6721 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
     6781 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt
     6841 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
     6901 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
     6961 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
     7021 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
     7081 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
     7141 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
     7201 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
     7261 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct
     7321 caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc
     7381 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     7441 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     7501 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
     7561 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     7621 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     7681 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta
     7741 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     7801 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
     7861 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
     7921 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
     7981 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     8041 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     8101 taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
     8161 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     8221 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     8281 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
     8341 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
     8401 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     8461 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
     8521 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
     8581 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     8641 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     8701 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     8761 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     8821 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
     8881 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     8941 attcacgaac gacggagaag ccttctgctt cgacaaactt ctgaatcgcc gcaccgattt
     9001 cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga
     9061 tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     9121 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     9181 gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     9241 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     9301 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     9361 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     9421 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     9481 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     9541 caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc
     9601 gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc
     9661 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     9721 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     9781 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     9841 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     9901 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     9961 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    10021 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    10081 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    10141 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca
    10201 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    10261 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    10321 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    10381 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    10441 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    10501 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    10561 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    10621 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
    10681 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    10741 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    10801 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    10861 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    10921 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    10981 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    11041 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    11101 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    11161 agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    11221 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    11281 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    11341 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    11401 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    11461 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    11521 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga
    11581 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    11641 ttaattatcg aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    11701 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    11761 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    11821 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    11881 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    11941 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    12001 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    12061 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    12121 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    12181 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    12241 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    12301 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    12361 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    12421 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
    12481 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    12541 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
    12601 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
    12661 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
    12721 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12781 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12841 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12901 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
    12961 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
    13021 cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg
    13081 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
    13141 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg
    13201 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
    13261 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
    13321 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
    13381 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
    13441 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
    13501 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
    13561 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
    13621 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg
    13681 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga
    13741 tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg
    13801 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
    13861 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
    13921 catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
    13981 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
    14041 gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg
    14101 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
    14161 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
    14221 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
    14281 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
    14341 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta
    14401 tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt
    14461 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
    14521 tatcgctgcg ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt
    14581 ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg
    14641 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
    14701 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
    14761 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
    14821 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
    14881 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
    14941 caagggagga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
    15001 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
    15061 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
    15121 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
    15181 cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat
    15241 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
    15301 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
    15361 gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
    15421 actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
    15481 tactttacag atgttctctg gcccgatttc gatgaacaag actttgaagg ggcgttaaat
    15541 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
    15601 tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
    15661 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc
    15721 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
    15781 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
    15841 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
    15901 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
    15961 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
    16021 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
    16081 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
    16141 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
    16201 ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
    16261 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
    16321 accgagagta tgtttaagcg agaagcagga attaaggaca gcggtcattt aattccagga
    16381 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    16441 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    16501 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    16561 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    16621 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg
    16681 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    16741 atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg
    16801 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    16861 tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    16921 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    16981 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca
    17041 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    17101 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    17161 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    17221 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    17281 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    17341 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    17401 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    17461 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    17521 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    17581 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    17641 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    17701 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    17761 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    17821 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    17881 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    17941 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat
    18001 gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    18061 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    18121 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    18181 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    18241 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    18301 atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    18361 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    18421 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    18481 gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct
    18541 gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    18601 tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta
    18661 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    18721 gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga
    18781 tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    18841 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    18901 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    18961 tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa
    19021 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    19081 gggtagcccg gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt
    19141 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    19201 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    19261 gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg
    19321 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    19381 caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg
    19441 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    19501 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    19561 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    19621 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    19681 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    19741 atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    19801 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    19861 tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    19921 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    19981 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    20041 cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga
    20101 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    20161 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    20221 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    20281 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    20341 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    20401 atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg
    20461 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    20521 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    20581 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga
    20641 aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg
    20701 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    20761 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    20821 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    20881 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    20941 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    21001 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    21061 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    21121 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    21181 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    21241 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    21301 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    21361 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    21421 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    21481 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    21541 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    21601 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    21661 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    21721 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    21781 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    21841 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    21901 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    21961 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    22021 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    22081 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    22141 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    22201 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    22261 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    22321 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    22381 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    22441 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    22501 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    22561 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    22621 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    22681 ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac
    22741 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    22801 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    22861 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    22921 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    22981 ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    23041 tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    23101 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat
    23161 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    23221 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    23281 ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    23341 atcctggggg ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt
    23401 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa
    23461 ctggcggtaa tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    23521 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    23581 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    23641 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    23701 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    23761 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    23821 gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg
    23881 agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg
    23941 cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag
    24001 cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg
    24061 atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc
    24121 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    24181 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    24241 ggcagagagt tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg
    24301 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    24361 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    24421 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    24481 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    24541 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    24601 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    24661 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    24721 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    24781 ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc
    24841 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    24901 gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg
    24961 gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    25021 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    25081 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt
    25141 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    25201 tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    25261 gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    25321 tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc
    25381 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    25441 ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    25501 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    25561 ttcttgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg
    25621 aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca
    25681 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    25741 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    25801 gcgagctgtt tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt
    25861 gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    25921 gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt
    25981 tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga
    26041 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    26101 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    26161 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    26221 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    26281 gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg
    26341 tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc
    26401 gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc
    26461 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    26521 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    26581 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    26641 cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    26701 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    26761 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg
    26821 ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    26881 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    26941 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga
    27001 tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac
    27061 agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc
    27121 aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata
    27181 tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    27241 ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc
    27301 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    27361 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    27421 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    27481 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    27541 acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    27601 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    27661 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    27721 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    27781 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    27841 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    27901 aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca
    27961 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    28021 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    28081 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    28141 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    28201 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    28261 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    28321 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag
    28381 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    28441 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    28501 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    28561 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    28621 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    28681 gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc
    28741 agattgcgca actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg
    28801 catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg
    28861 tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc
    28921 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    28981 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    29041 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    29101 cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg
    29161 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    29221 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    29281 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    29341 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    29401 tggcggtatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    29461 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    29521 caaaagttct gaaaaagggt cacttcgg
//
LOCUS       DAZPLI010000036        28893 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZPLI010000036 DAZPLI010000000
VERSION     DAZPLI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28893
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.036"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..1262)
                     /gene="ipaHb"
                     /locus_tag="ACHUU3_001559"
     CDS             complement(<1..1262)
                     /gene="ipaHb"
                     /locus_tag="ACHUU3_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaHb"
                     /protein_id="HFR2081663.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFTV
                     AADATESCEDRVALTWNNLRKTLLVHQAS"
     gene            1753..2310
                     /gene="pinE"
                     /locus_tag="ACHUU3_001560"
                     /pseudo
     CDS             1753..2310
                     /gene="pinE"
                     /locus_tag="ACHUU3_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2649..3497)
                     /locus_tag="ACHUU3_001561"
     CDS             complement(2649..3497)
                     /locus_tag="ACHUU3_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR2081664.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3706..4341
                     /gene="yfhb"
                     /locus_tag="ACHUU3_001562"
     CDS             3706..4341
                     /gene="yfhb"
                     /locus_tag="ACHUU3_001562"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR2081665.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4399..4902
                     /gene="tadA"
                     /locus_tag="ACHUU3_001563"
     CDS             4399..4902
                     /gene="tadA"
                     /locus_tag="ACHUU3_001563"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR2081666.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQPSTD"
     gene            complement(4899..6455)
                     /gene="mltF"
                     /locus_tag="ACHUU3_001564"
     CDS             complement(4899..6455)
                     /gene="mltF"
                     /locus_tag="ACHUU3_001564"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR2081667.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINWKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDCTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6713..10600
                     /gene="purL"
                     /locus_tag="ACHUU3_001565"
                     /gene_synonym="purI"
     CDS             6713..10600
                     /gene="purL"
                     /locus_tag="ACHUU3_001565"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR2081668.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            11158..12585
                     /gene="qseE"
                     /locus_tag="ACHUU3_001566"
     CDS             11158..12585
                     /gene="qseE"
                     /locus_tag="ACHUU3_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR2081669.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12750..13463
                     /gene="qseG"
                     /locus_tag="ACHUU3_001567"
     CDS             12750..13463
                     /gene="qseG"
                     /locus_tag="ACHUU3_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR2081670.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13453..14787
                     /gene="glrR"
                     /locus_tag="ACHUU3_001568"
     CDS             13453..14787
                     /gene="glrR"
                     /locus_tag="ACHUU3_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR2081671.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14848..15186
                     /gene="glnB"
                     /locus_tag="ACHUU3_001569"
     CDS             14848..15186
                     /gene="glnB"
                     /locus_tag="ACHUU3_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR2081672.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15231..16421)
                     /gene="hmpA"
                     /locus_tag="ACHUU3_001570"
     CDS             complement(15231..16421)
                     /gene="hmpA"
                     /locus_tag="ACHUU3_001570"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR2081673.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16749..18002
                     /gene="glyA"
                     /locus_tag="ACHUU3_001571"
     CDS             16749..18002
                     /gene="glyA"
                     /locus_tag="ACHUU3_001571"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR2081674.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(18200..19393)
                     /locus_tag="ACHUU3_001572"
                     /pseudo
     CDS             complement(18200..19393)
                     /locus_tag="ACHUU3_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19511..22788
                     /locus_tag="ACHUU3_001573"
                     /pseudo
     CDS             19511..22788
                     /locus_tag="ACHUU3_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22885..23868
                     /locus_tag="ACHUU3_001574"
     CDS             22885..23868
                     /locus_tag="ACHUU3_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFR2081675.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23891..25402
                     /locus_tag="ACHUU3_001575"
     CDS             23891..25402
                     /locus_tag="ACHUU3_001575"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR2081676.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25427..26425
                     /locus_tag="ACHUU3_001576"
     CDS             25427..26425
                     /locus_tag="ACHUU3_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR2081677.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26458..27552
                     /locus_tag="ACHUU3_001577"
     CDS             26458..27552
                     /locus_tag="ACHUU3_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR2081678.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHCATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27564..28436
                     /locus_tag="ACHUU3_001578"
     CDS             27564..28436
                     /locus_tag="ACHUU3_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR2081679.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28484..>28893)
                     /locus_tag="ACHUU3_001579"
     CDS             complement(28484..>28893)
                     /locus_tag="ACHUU3_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR2081680.1"
                     /translation="YFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMASL
                     GAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDAV
                     GPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6867 a         7143 c         7864 g         7019 t
ORIGIN      
        1 gatgcctgat ggaccaggag ggttttccgg agattgttcc atgtgagcgc gacacggtcc
       61 tcacagctct cagtggcatc agcagcaaca gtgaaagact gctgtcgaag ctccgcagag
      121 gcactgagtt tttccagcca tgcagcgacc tgttcacgga atccggaggt attgcgtgca
      181 gagacggtat cggaaaggcg gtcaaggaac gcggagaatg cccgggaatt ctcctcggtg
      241 gaaaaagaag tccattgtgt agaaacctcc cgacgatctg cttcaggaaa ccaggctgcc
      301 acagtttcgg gaagaggggg gatctgaaag ttctgccccc cttcagtgat gactctgata
      361 tggcgcgaat gtaaattttg cggcaacatc tggcgtaaaa acagtctgtt ttggtctgac
      421 agggaaatac gaaactgagg cccgttaaaa tttggaaccg aagtttgctg tattaagaat
      481 agcagagttt gatctgataa ggggttattt gcaatctcaa tagttatatg aggatctaaa
      541 cgaaaaagat ttgtcgggat tgtagttatt tggttgtttt caaaataaaa aaatcttctc
      601 atgataatat tttcttggat gtttggtaag ttgcttaatc tattgctata tgcaaaaaga
      661 agttgcagtc tcctaggtaa agggggcagt tcagtaagat ggttgtgcgc aaccttaagt
      721 atcttaagat tagaaggcag agatggaaga gtttgcagtt cgttgttcct aacctcaagt
      781 ttttttaaat tcaagggtaa tgaaggcagt gtggataacc tattatactc tgcgcttagt
      841 gatttgatgg tgtctggtaa taacggcaga ttctctaatt gattttcgga cacatccaaa
      901 gttattagat aatgtggtaa tgcagatata tgtgttaaga aattatctct tgcaataagt
      961 tctttaatat tttcaggtaa atcagtaagg atggttaatt tattacagct cacatcaagc
     1021 tttttaatgc aagcagggag tacaggtaac gaggaaagcg ccaaggctcg caggtccagt
     1081 ttatgcccct tagaggtaag acatgcacga agtcttctta ccgcctcagt acgctgttct
     1141 tccggtaacc cctgtttctc ccaatcatcc cattcggaca aataatggtt gggggatgta
     1201 attgtgctga tatgtggaaa taaacaggat gaaatatttc tgagcatatt aatttctcgc
     1261 atatggatat ggaattagat tgtgaataaa ttgtatttaa tgtatctctc ctaatgagga
     1321 gggtgattca gaatattcgc cataagggaa tgagtgttat atcatgcttt ctaccctggt
     1381 aagtatattt gcttttttcg tatatttggt tcatcttttt ttgatgtttt acatattcac
     1441 aatgcagagg atttatacca gaagacatta gtcttgagta agatactaca acatctccaa
     1501 ctattttagc cagcctgact acttgacttg gtaatatttg ttgctaataa tcatgtataa
     1561 aataagctta tatacatagt aacttcagca aaggatttag taatgatcca aatcatatct
     1621 caatgtactg tttttttctt taaaaaaaca gtacattcta ctttataatg actgtgtatt
     1681 cccatggaag gagatcaccc aattctcccc cccgactttt gacgcagtgt cttgttaaca
     1741 gggggattta ccatgcttat tggctatgta cgcgtatcaa caaatgacca gaataccgat
     1801 ctacaacgta atgcgttgaa ctgtgcagga tgcgagctga tttttgaaga caagataagt
     1861 ggcacaaagt ccgaaaggcc ggggctgaaa aaactgctca ggacattatc ggcaggcgac
     1921 acactggttg tctggaagct ggatcggctg gggcgcagta tgcggcatct tgtcgtgctg
     1981 gtggaggagt tgcgcgaacg aggcatcaac tttcgtagtc tgactgattc aattgatacc
     2041 agcacaccaa tgggacgctt tttcttccat gtgatgggtg ccctggctga aatggaacgt
     2101 gaactgattg ttgaacgaac aaaagcagga ctggaagctg ctcgcgcaca gggacgaatt
     2161 ggcggtcgcc gtcccaaact ttcaccagaa taatgggcgc aggccgggcg actaattgca
     2221 tcaggagttt ctcgccagaa ggtggcgatc atctatgatg ttggcgaatc gacttttttg
     2281 tataagaggt ttccagccag ggttatataa aaccgcagac acggcgtata caagaacgtg
     2341 ctacggttga ctgaaaagtt aatgtggtat gtcacaggcc ttttgcgccg taaagccccg
     2401 tcctttaggg cgcggaggac gttaaagaaa gtggtgttct ttgtgttgct gaacttatgc
     2461 aaaatttttt tgcaccataa atccaacaac acattcaata acacattgct gtatcactat
     2521 gaaaataaat gtgtttttat ttgatttcac acatatcaca attgatgcat tttttagtga
     2581 ttaacaacgc cataaggaaa ctctcaaaac aacacaaacc gggcgggcat tgtacgcgca
     2641 ttctttactc agaccagttt tttcaccagc gcttcactat gacgaatacg ttctggtgcc
     2701 agttccagat cctgctgaat cagcgcaatg aacagcaaat ccgttaacat tccctgagcg
     2761 tgacaagcag agattgaagc actgtttgtc gcttgttctt cggcaatggt atacaggcaa
     2821 tgagaagcac gctgttgcag ggcattcgga gtaaagccgg taatcgccag cacttttccg
     2881 cccactcgca gcatctcatc tgccgccagg tttaactcgc gtcgtacacc ggtgtaggaa
     2941 atggctaata acagatcgtc aggggacgac gcctgtactg ttgcgagtag cgcatgcata
     3001 tcgcgcactg cggcagcatt gaagccaatc ttcatcagct tccaggcaaa gttttgcgcc
     3061 accagacccg aagcgccaat accggtcaga attatccgcc gcgcagagcg caacattgtt
     3121 acgcattcat gcagtttctc ttcactatta acgtttagcg ttgcgtacat agcggcagta
     3181 ttttctttaa tcagtttttc gccgaccagc cgtaacggat catcaccgcg gatttggtta
     3241 tgaatgggca cggagggcga ttccggctgg cttgccagcg cttcactcaa cgccaactta
     3301 agcgccggaa aacctttata gccgagtttt tgcgcgaact tcacgacgct ggactgactg
     3361 actccggctt cgttggccag ttgctgagag cttaaatggc gcgccgtatc aggttgtagc
     3421 agcagataat ccgccagttt tttatcactc tgggcaagcc cctggtaacg ctgacggata
     3481 cgaagtaagc cgttcatatc tttcctcgcg atgaaatagt ggcgacaaca ggcaatatga
     3541 gacacacgcg agtgaatttt atattccatt gccgctaatt gccatgaatt aaaaattcca
     3601 gttagccatg atgcggtcga ttgttgccgg aaaagtgatg atctatctgt taggctatgt
     3661 agtagcgaaa attaatcagg atgtttcagt ccagaggagt atggtttggc aactcacgag
     3721 cgtcgtgtgg tgttttttga cttagatgga acattgcatc agcaggatat gttcggcagt
     3781 tttctgcgct atttactgcg tcgccaaccg ctgaatgcgt tacttgtcct gccgttgtta
     3841 ccgattatag ccattgcgtt attgataaaa ggtcgtgcgg cacgctggcc gatgagtctg
     3901 cttctgtggg ggtgcacttt tggtcacagc gaagcacgtt tacagacgtt gcaggccgat
     3961 ttcgtgcgct ggtttcgcga caatgttacc gcctttccgc tggttcagga gcgattaacc
     4021 acctacctgt taagttccga tgctgatatc tggttgatta ccggctctcc gcagccgctg
     4081 gttgaagcgg tttatttcga tacgccctgg ctgccgcggg ttaatcttat cgccagccaa
     4141 attcagcgtg gctatggtgg ttgggtattg acgatgcgtt gtctgggaca tgaaaaggtc
     4201 gcacaactgg agcgcaaaat cggcactccg ctgcggctgt acagtggcta tagcgacagt
     4261 aatcaggaca atccgctgct ttatttctgt cagcatcgtt ggcgagtaac cccgcgcggt
     4321 gaactccagc aactggaata gagtaaagca tagcgtccgt gtataatgcg ccgcgctttt
     4381 ataaccggag ttttcttttt gtctgaagtc gaatttagcc acgaatactg gatgcgtcac
     4441 gcgctgacgc tggcgaaacg tgcctgggat gagcgggaag tgccggtcgg cgcggtatta
     4501 gtgcataaca atcgggtaat cggcgaaggc tggaaccgcc cgattggtcg ccatgatccc
     4561 accgcacatg cagaaatcat ggccctgcgg cagggtggtc tggtgatgca aaattatcgt
     4621 ctgatcgacg ccacgttgta tgtcacgctt gaaccatgtg taatgtgtgc cggagcgatg
     4681 atccacagtc gcattggtcg cgtggtcttt ggtgcgcgtg acgcgaaaac tggcgctgcg
     4741 ggatctttaa tggatgtgct gcatcatccg ggtatgaatc accgagtgga aattacggaa
     4801 ggaatactgg cggatgagtg cgcggcgttg ctcagtgact tctttcgcat gcgccgccag
     4861 gaaattaaag cgcagaaaaa agcgcaaccc tcgacggatt aattttgttt ctcttcactc
     4921 cctttcctgg aaaacagcag agagggagaa tgggtcaaat agtttgatga cgactgggaa
     4981 agaaacgaga gaaaaggaaa tttctctttg cccaactccg taggcgatac cgccggataa
     5041 tcctgcgcca gttgcatcgc cgcttctgta gcctgcttct ctttctcttg cagataaccc
     5101 accaggctaa tctgatactt acgaatattt tcgacataag cgtaggcttc atgtccacga
     5161 gcgtagccgt aagtcagctt gctgtaatag ggtttctggc taagtaaagg cagacgctgt
     5221 tttacgtcag cccaactgtc aggattccct ttggtttttg ccgtcagggc gcgggcatcc
     5281 agcatatgcg catagcccat attgtacgca gcgagggcaa accagatccg ctcgttctcc
     5341 ggcacacttt ccggcacttt actcatcata tcctgcaaat aacgcacgcc accgctgatg
     5401 ctctgttcgg catcggtacg atccgtaatg ccgaggcttt gcgcggtatt tttggttaac
     5461 atcatcatgc cgcgcacacc cgtcggtgaa gtggcctgtg catcccagtg cgattcctga
     5521 taagcaatag cggccagcaa acgccagtca atttcttcgg cgtatttctc aaacaggggc
     5581 tttaactgcg gcagtaccgc atcgacggcg cgtaaaaatg tgcgcgtatc gacgtaatca
     5641 aaatcatcgc catgccccag gtatttctct tcaatgcgtg ccagcgtaca gtcttcattc
     5701 atttcgttga agaagtcgag cagggcggcg gaaagggtat tatcgccatc taacgggcta
     5761 aaccaagtca ccggttgttc atcggtgata tcgagcgcta cggcgagctc cgggtgaacg
     5821 cgctgaaaca ggctgatggc gacagaatca gcaatggtgt aatcgagttt tccttcgatg
     5881 acatcttcca ttaattccgc agagcctttt ttgtcgtcta ccttccagct taattccggg
     5941 aattttgtgt ctttcagggt ttggagatcg ttaaccacca cgtgacccgg tgcaacggtg
     6001 agttgctccg ccgtcaggtt gcccagtgtg cgcgggcgat actgactcac tttataaacc
     6061 agttgttgtg acacggaata ataggtaggg ccaggctgat aattttttac ccgctcactg
     6121 ttatagacca gtcctgccgc cagcaggtcg gcgttaccat tatcgaggtc gtcaaacagc
     6181 tggctgatat tctgccgcac ggtcactttc agttttacac cgaggtaatc ggcaaactgt
     6241 ttcgccagtt cgtaatccag gccaaaaggt ttccagttga tttcgttata agtcagggga
     6301 gtatgaatgg tgctcacacg caactctccc cgcgcttgaa tggcggcgat acggttgtcg
     6361 gctttaccaa accagggaat ggatggccag agagcgaccg cgagcagcag tgccagaatg
     6421 ccgatgaaca gataattaat ctttaatttt ttcaattagt taattctctg tgtcgtgcgc
     6481 gtcccagctt gaaaaaacgt aataatagtg aaaggtttac tcataaatga gcggcatttt
     6541 gcgtaaacct gcgccagatg gcaacttatt acagccattg gcggcacgcg ttgctaattc
     6601 acgatggtga ttttatttcc acgcaaacgg tttcgtcagc gcatcagatt ctttataatg
     6661 acgcccgttt cccccccttg ggtacaccga aagcttagaa gacgagagac ttatgatgga
     6721 aattctgcgt ggttcgcctg cactgtcggc attccgaatc aacaaactgc tggcacgttt
     6781 tcaggctgcc aggctcccgg ttcacaatat ttacgccgag tatgtccatt ttgctgacct
     6841 caatgcgccg ttaaacgatg atgagcacgc acaacttgaa cgcctgctga aatatggccc
     6901 ggcactcgcc agccacgccc cgcaaggcaa actcctgctg gtgaccccgc gtcctggcac
     6961 catctctccc tggtcttcga aagcgaccga tattgcccat aactgcgggc tacaacaggt
     7021 aaaccgcctt gagcgcggcg ttgcttacta tatagaagcc ggtacgctga ccaatgaaca
     7081 atggcagcag gttaccgctg aactgcacga ccgcatgatg gaaacggtct tttttgcttt
     7141 agatgatgca gagcagttgt ttgcccacca tcaaccgact ccggttacca gcgttgattt
     7201 gctggggcag ggccgtcagg cgctgatcga cgctaacctg cgtcttggcc tggctctggc
     7261 ggaagatgaa attgactatc tgcaggatgc tttcacaaag cttggtcgta acccgaacga
     7321 catcgagctg tatatgttcg ctcaggcgaa ctctgagcac tgccgccaca aaatttttaa
     7381 tgccgactgg attatcgacg gtgaacagca gccgaaatcg ctgttcaaga tgatcaaaaa
     7441 tactttcgaa accacgccag atcacgttct ctctgcttat aaagataacg ccgccgtaat
     7501 ggaaggttct gaagtgggcc gctactttgc tgaccacgaa acgggccgct acgatttcca
     7561 tcaggaaccg gcgcatattc tgatgaaagt cgaaactcac aaccacccga cggcgatttc
     7621 tccgtggccg ggcgcggcga cgggttccgg cggcgaaatt cgtgatgaag gcgccaccgg
     7681 acgcggtgcg aagccgaagg ctggtctggt tggtttctcc gtttccaacc tgcgaattcc
     7741 tggcttcgaa cagccgtggg aagaagattt cggtaagcct gagcgcattg tcaccgcgct
     7801 ggacatcatg accgaaggcc cgctgggcgg cgcggcgttt aacaacgaat ttggtcgtcc
     7861 ggcactgaat ggctacttcc gtacttatga agaaaaagtg aacagccaca acggcgaaga
     7921 gctgcgcggt tatcacaaac cgatcatgct ggcgggcggg atcggcaaca ttcgcgccga
     7981 tcacgtacaa aaaggcgaga tcaacgtcgg tgcgaagctg gtcgttctcg gcggcccggc
     8041 aatgaacatc ggtcttggcg gcggagcggc gtcttccatg gcgtcaggtc agtctgatgc
     8101 cgacctcgac tttgcttccg tacagcgcga caacccggag atggagcgtc gctgccagga
     8161 agtgatcgac cgttgctggc agcttggtga tgccaaccca atcctgttta tccacgacgt
     8221 cggcgctggc ggtctttcta acgcgatgcc ggaactggtg agcgacggcg ggcgcggcgg
     8281 taaatttgaa ctgcgcgata ttctaagcga cgaaccgggc atgagcccgc tggaaatctg
     8341 gtgtaacgaa tcccaggaac gctacgtgct ggcggttgct gccgatcaat taccgctgtt
     8401 tgacgaactg tgtaagcgtg agcgcgcacc ctacgcggtg attggtgaag cgaccgaaga
     8461 actgcatctt tctctgcacg atcgtcattt tgataatcag ccgatcgatc tgccgctgga
     8521 cgtcctgctt ggtaaaacgc cgaagatgac ccgcgatgta caaacgctga aagcgaaggg
     8581 cgatgcgctg gtccgtgaag ggatcaccat tgcagacgcg gtgaaacgcg tactgcatct
     8641 gccgaccgtg gcagagaaaa ccttcctggt gaccatcggc gaccgcagcg taaccggcat
     8701 ggtagcgcgc gatcagatgg tggggccgtg gcaggtgccg gtcgctaact gcgcggtcac
     8761 taccgccagc ctcgacagct actacggtga agcgatggcg attggcgagc gtgcgccggt
     8821 tgcactgctg gatttcgccg cctctgcccg tctggcggtc ggtgaagcgt taaccaatat
     8881 tgccgcaaca cagattggcg atatcaaacg cattaaactt tccgccaact ggatggcggc
     8941 ggcaggccac cctggtgaag atgcgggcct gtatgaagcc gttaaagccg tgggcgaaga
     9001 gctttgtccg gcgctgggcc tgacgatccc ggtgggtaaa gactctatgt cgatgaaaac
     9061 ccgctggcag gaaggtaacg aagagcgcga aatgacgtcg ccgctgtcgc tggtgatttc
     9121 tgcatttgcc cgcgtggaag atgtgcgtca caccatcacg ccacagcttt ctaccgaaga
     9181 taacacactg ctgctgattg atttgggcaa aggcaataac gcgctgggcg caacggcgct
     9241 ggcgcaggtt tatcgtcagc ttggcgacaa accggcagat gtacgcgacg tcgcgcaact
     9301 gaaaggcttc tatgacgcga ttcaggcgct ggttgcacag cgtaagctgc tcgcatacca
     9361 cgaccgctct gatggcggcc tgctggtaac gctggcggaa atggcctttg ctggtcattg
     9421 cggcattaac gcggatattg cctctctggg cgacgatcgc ctggcggcgt tgtttaacga
     9481 agaactgggt gcggtgattc aggttcgtgc cgctgaccgt gaagcggtcg agtccgtact
     9541 ggcacagcat gggcttgctg attgtgtcca ttatgtaggg caggcggttt ccggtgaccg
     9601 ttttgtgatt accgccaacg ggcagactgt attcagcgaa agccgcacca cgttgcgtgt
     9661 ctggtgggca gaaactacct ggcagatgca gcgcctgcgt gacaacccgg agtgtgctga
     9721 ccaggagcac caggcgaaat caaacgacgc cgatccgggc ctgaatgtga aactgtcgtt
     9781 cgatatcaac gaagatgtgg cagcaccgta tattgccact ggcgcacgtc cgaaagttgc
     9841 tgtgctgcgt gagcagggcg tgaactcgca tgttgaaatg gcggcagctt tccaccgtgc
     9901 aggctttgat gctatcgacg tgcatatgag tgacctgctg accggacgca cgggcctgga
     9961 agatttccac gccctggtcg cgtgcggtgg tttctcctac ggtgatgtgc tgggtgccgg
    10021 tgaaggttgg gcgaagtcaa tcctgttcaa tgaccgtgta cgcgatgagt ttgcaacctt
    10081 cttccaccgt ccgcaaacgc tggcgctggg ggtatgtaac ggttgccaga tgatgtctaa
    10141 cctgcgtgaa ctgatcccgg ggagcgagtt gtggccacgt tttgtgcgta acacctccga
    10201 tcgctttgaa gcgcgtttca gcctggttga agtaacccaa agcccgtctc tgctgttgca
    10261 ggggatggtg ggctcgcaaa tgccgattgc tgtctctcat ggtgaagggc gcgtggaagt
    10321 gcgtgatgcg gcgcatctgg cggcactgga aagcaaaggg ctggtggcac tgcgctatgt
    10381 cgataacttc ggcaaagtca ctgaaaccta cccggctaac ccgaacggtt caccgaacgg
    10441 tattacggcg gttacgactg aaagtggtcg agtcaccatt atgatgccgc acccggaacg
    10501 tgttttccgt actgtcagca actcctggca tccggaaaac tggggcgagg atggcccatg
    10561 gatgcgcatt ttccgcaatg cgcgtaagca gttggggtaa gtcatcagtt cattggtttt
    10621 gcagcccgga tgcggcatta agcaccaacc actatcataa gctcccaact ccgggagctt
    10681 ttttgtgtct gtaaatcacg acaatgggtg gtttaccgtg tcgctttctg gcgacactta
    10741 aatgattgat tttattgtaa tctaataagt ttatcttaag ttgtctcttt ttagcgacac
    10801 agtggctgat ttacattcaa atagcgaaga agccataaat ccctcaaagc aacgtataat
    10861 cagtacgtta ccaaactatt ttctttattg gcacagttac tgcataatag taagcagtgg
    10921 ctcattcacc gacttatgtc agccccttcg ggacgtgcta cataaaattc gaatgacgca
    10981 caacaaggtg cctgccgtcc aacttctgat atcagcgtag ctatatcaac catcgggcga
    11041 aacgtcgagt taggcaccgc cttattccat aacaaagccg ggtaattccc ggctttgttg
    11101 tatctgaact tcccctcggt tagcatcagg ctattcgcgt ctgacgagag taacaccttg
    11161 aaacgctggc ccgtttttcc ccgctcatta cgacaactgg taatgctggc atttttgcta
    11221 attctactac ccctgttggt gctggcatgg caagcctggc aaagcctgaa tgcgcttagc
    11281 gatcaggcgg cgctggttaa ccgcactacg cttattgatg cccggcgtag tgaagcgatg
    11341 accaacgcgg cgctggagat ggagcgtagc taccgtcagt attgcgtgct ggacgaccct
    11401 acgcttgcga aggtttatca aagccagcgc aagcgttaca gcgaaatgct cgatgcccac
    11461 gcaggcgtac tgccagacga taaactttac caggcattgc gtcaggattt gaacaatctg
    11521 gcccaacttc agtgtaacaa cagtggtccc gatgctgccg ccgccgcgcg tcttgaagcc
    11581 tttgccagtg ccaataccga aatggtgcag gccacgcgca cagtggtgtt ctctcgtggg
    11641 cagcaacttc agcgtgaaat cgccgaacgt gggcaatatt ttggttggca atcgctggtg
    11701 ctatttctgg tgagtctggt gatggtgcta cttttcacgc ggatgattat cgggccggtg
    11761 aaaaatatcg agcgcatgat caaccgtctg ggggaagggc gttctctggg caatagcgtt
    11821 tcgttcagtg gaccgagcga gttacgctcg gttgggcaac gtattctttg gttaagtgag
    11881 cgcctgtcat ggctggaatc ccaacgccat caatttttaa gacatttatc tcatgaatta
    11941 aaaacgccac tggcgagtat gcgcgagggc actgaattac tggctgacca ggttgtcggg
    12001 ccgcttacgc cagagcaaaa agaggtggtg agcattcttg atagcagcag ccgcaatttg
    12061 caaaaactga tcgaacaact gcttgattac aaccgtaaac aggcggacag tgcggtggaa
    12121 ctggagaatg ttgaattagc accgctggtg gagacagtgg tttctgctca tagcctgccc
    12181 gcacgggcta aaatgatgca taccgacgtc gatctcaaag caacagcttg cctggcggag
    12241 ccaatgctgc tgatgagcgt actggataat ctttactcca atgcggtgca ctacggggct
    12301 gaatccggta acatttgcct tcgcagcagt tcacatggtg cgcgggttta tattgatgtc
    12361 atcaatacag gcacgcccat tccgcaagag gaacgcgcca tgatcttcga accctttttt
    12421 cagggaagcc accagcgaaa aggggcggtg aagggcagcg gtctgggatt aagcattgcc
    12481 agggattgta ttcgccgtat gcaaggggaa ctgtatctgg tcgacgagag cgggcaagac
    12541 gtttgtttcc gcattgaatt accgtcgtcg aaaaacacga aataatgcta acagcctatc
    12601 ctatcaggct attttatttg tcagttcggc cccgggcagt gctcgaaatc ttcacgtacg
    12661 atatgtacgc tccggtttct ccgcgctgtc catgtccaga ctgtctgcaa caattacgcc
    12721 cactgggata ggctttaagt ctggtgaata tgcgacacat ttttcaacga ttattgcccc
    12781 gacggctgtg gctggcaggg ctgccatgtc tggcattgct gggttgcgtg cagaatcaca
    12841 ataagccagc cattgatacg ccagcagaag aaaaaattcc ggtttatcaa ctggctgact
    12901 atctttctac tgaatgtagc gacatctggg cgctgcaagg taaatcaacc gaaaccaatc
    12961 cgctttactg gctgcgggcg atggattgtg ctgatcgttt aatgcctgcg cagtcgcgtc
    13021 agcaagctcg ccaatatgac gacggcagct ggcaaaatac ctttaaacaa gggattttgc
    13081 tcgccgacgc caaaattacg ccgtatgagc gccgccagct ggtcgcgcgt attgaagcgt
    13141 taagtaccga gatcccggca caggttcgcc cactgtatca actctggcgc gacggtcagg
    13201 cgctgcaact gcaactggcg gaagagcgcc aacgttacag taaactccag cagtccagcg
    13261 acagcgagct ggatacattg cgccagcaac atcacgttct acagcagcaa ctggaactca
    13321 ccacccgcaa gctggaaaac ctgaccgata ttgaacgcca gctctcgacc cgcaaaccga
    13381 ctggaaattt ctcgccggat acgccgcacg agagtgaaaa gcccgcgcca tccacccatg
    13441 aggtcactcc tgatgagcca taaacctgcg catttattat tggtcgatga cgatccggga
    13501 ttgctgaaac tgcttggcct gcgcctgacc agcgaaggct acagtgtggt cacggcggaa
    13561 agtggcgctg aaggattacg ggtactgaat cgcgaaaaag tagatttagt tatcagcgac
    13621 ctgcggatgg atgaaatgga cggtatgcag ctatttgctg aaatccagaa agtgcagccg
    13681 ggaatgccag taattattct tactgcgcat ggttctatcc ccgatgccgt tgctgcaaca
    13741 cagcagggcg tttttagttt cctcaccaag cctgtcgata aagatgcgct atatcaggca
    13801 attgacgatg cgctggagca atccgcgcca gccaccgatg aacgctggcg cgaggcaatt
    13861 gtcacccgca gcccgctgat gctgcgtttg ctggaacagg cgcggctggt ggcgcaatca
    13921 gacgtcagcg ttttgattaa cggtcagagc ggcaccggga aagagatttt cgcccaggct
    13981 atccacaacg ccagcccgcg caacagcaaa ccatttattg ctattaactg tggcgcattg
    14041 cccgagcaat tgctggagtc ggagctgttt ggtcatgcgc gtggcgcgtt tactggcgct
    14101 gtcagcaatc gcgaaggttt attccaggcg gcggaaggcg gaacgctatt tctcgatgaa
    14161 attggcgata tgcctgcgcc gttacaggtc aaactgctgc gcgtgttgca ggagcgtaaa
    14221 gtgcgcccgc tgggcagtaa ccgcgatatt gatatcaatg tgcggattat ttctgccact
    14281 caccgtgatc tgccaaaagc gatggcgcgc ggggaattcc gcgaagatct ctattaccgc
    14341 ctcaacgttg tcagcctgaa aattccggca ctggcggagc gcacagaaga cattccgcta
    14401 ctggcaaatc acctgttgcg ccaggcggca gagcgacata aaccgtttgt ccgcgcgttc
    14461 tctaccgatg cgatgaaacg cctgatgacc gcgagctggc cgggtaatgt gcgccagttg
    14521 gtcaacgtga ttgaacagtg cgtggcgctg acctcatctc cggtgattag tgatgcgctg
    14581 gtggagcagg cgctggaggg tgaaaatacg gcgctgccaa cctttgttga ggcacgtaat
    14641 cagtttgaac tcaactattt gcgtaagctg ctgcaaatca ccaaaggcaa cgtcacccac
    14701 gcggcgagaa tggcggggcg caaccggaca gaattttata aactgctttc ccgacacgag
    14761 ctggatgcaa acgatttcaa ggaatgaatt ggcgttatgt gttacgttta gcagatcaaa
    14821 agacaggcga ccttttcaag gaatagcatg aaaaagattg atgcgattat aaaacccttc
    14881 aagctggacg atgtccgcga agcactggcc gaagtcggta ttaccggcat gacggtgacc
    14941 gaagtgaaag gctttggtcg ccagaaaggc cataccgagc tgtaccgcgg cgcggagtat
    15001 atggtggatt ttctgccgaa agtgaaaatt gagattgtcg taccggacga cattgtcgat
    15061 acctgtgtcg ataccattat tcgcacggcg caaaccggca aaatcggtga cggtaaaatc
    15121 ttcgtctttg acgtggcacg ggtcattcgc atccgtaccg gtgaagagga cgacgcggca
    15181 atttaaaccg cgtcaagggt tgccggatgt ttccatccgg caacatcaaa ttacagcacc
    15241 ttatgcgggc caaagcattc gtaatgaatg ttttcctgct tcacgcccag atccactaac
    15301 tgtttcgcgg taaactgcat gaagccaacc gggccgcaga gatagaactg cattgtcgga
    15361 tcgctgaacg caccttccag tttgctcaaa tccatcagac cttcgctatc aaactgacct
    15421 ttagcgcgat cggcttcgct cggctgacga tgccaggtgt gcgcggtaaa gcgcggcagt
    15481 gactgcccca gttccttaac ttcatcggca aaggcgtgaa catcgccatt ttctgccgca
    15541 tggaaccagt tcacttgtgc tgtgtggcct gcttttgcca gcgtgtcgag cattgccagc
    15601 attggcgttt gaccaacacc ggcagagatt aacgtcactg gtgtgtcatc tgcgacagcc
    15661 ataaagaaat cacctgccgg agcgaccagt ttcacgacat cgccaacatt ggcgtgattg
    15721 tgcaaccagt tggatacctg cccaccctct tcgcgtttca ccgcaatacg atagcctttg
    15781 ccatccggtt tgcgagtcaa agagtactga cgaatttcct gatgcgggaa accttccggc
    15841 ttcagccaga cgccgagata ttgccccgga cggtattctg ccactgcgcc accgtcgacc
    15901 ggctccagtt cgaagctggt gataagcgcg ctgcgcggtg ttttagccac aatgcggaaa
    15961 tcgcgagtac cttcccaacc accggctttg ctggcgtttt cgttatagat ttccgcctcg
    16021 cgattgataa atacattagc cagtacacca taggctttac cccacgcgtc cagcacttcc
    16081 tgccccgggc tgaacatttc gtccagcgtt gccaacaggt gttcaccgac gatgttgtac
    16141 tgttccggtt tgatctggaa gctggtgtgc ttctgcgcga ttttttctac cgctggcagc
    16201 agcgcaggca ggttttcaat attactggcg taggcggcaa tagcgttaaa cagggcttca
    16261 cgttgatcgc cattacgctg gttactcatg ttaaaaattt ctttgagttc tgggttatga
    16321 gtaaacatac ggtcgtagaa atgggcggtt aactttggcc ccgtttccac cagtaaaggg
    16381 atggtggctt ttactgtagc gatggtttga gcgtcaagca tatggtcttc ctttttttgc
    16441 atcttaattg atgtatctca aatgcatctt ataaaaaata gccctgcaat gtaaatggtt
    16501 ctttggtgtt tttcagaaag aatgtgatga agtgaaaaat ttgcatcaca aacctgaaaa
    16561 gaaatccgtt tccggttgca agctctttat tctccaaagc cttgcgtagc ctgaaggtaa
    16621 tcgtttgcgt aaattccttt gtcaagacct gttatcgcac aatgattcgg ttatactgtt
    16681 cgccgttgtc caacaggacc gcctataaag gccaaaaatt ttattgttag ctgagtcagg
    16741 agatgcggat gttaaagcgt gaaatgaaca ttgccgatta tgatgccgaa ctgtggcagg
    16801 ctatggagca ggaaaaagta cgtcaggaag agcacatcga actgatcgcc tccgaaaact
    16861 acaccagccc gcgcgtaatg caggcgcagg gttctcagct gaccaacaaa tatgctgaag
    16921 gttatccggg caaacgctac tacggcggtt gcgagtatgt tgatatcgtt gaacaactgg
    16981 cgatcgatcg tgcgaaagaa ctgttcggcg ctgactacgc taacgtccag ccgcactccg
    17041 gctcccaggc taactttgcg gtctacaccg cgctgctgga accaggtgat accgttctgg
    17101 gtatgaacct ggcgcatggc ggtcacctga ctcacggttc tccggttaac ttctccggta
    17161 aactgtacaa catcgttcct tacggtatcg atgctaccgg tcatatcgac tacgccgatc
    17221 tggaaaaaca agccaaagaa cacaagccga aaatgattat cggtggtttc tctgcatatt
    17281 ccggcgtggt tgactgggcg aaaatgcgtg aaatcgctga cagcatcggt gcttacctgt
    17341 tcgttgatat ggcgcacgtt gcgggcctgg ttgctgctgg cgtctacccg aacccggttc
    17401 ctcatgctca cgttgttact accaccactc acaaaaccct ggcgggtccg cgcggcggcc
    17461 tgatcctggc gaaaggtggt agcgaagagc tgtacaaaaa actgaactct gccgttttcc
    17521 ctggtggtca gggcggtccg ttgatgcacg taatcgccgg taaagcggtt gctctgaaag
    17581 aagcgatgga gcctgagttc aaaacttacc agcagcaggt cgcgaaaaac gctaaagcga
    17641 tggtagaagt gttccttgag cgcggctaca aagtggtttc cggcggcact gataaccacc
    17701 tgttcctggt tgatctggtt gataaaaacc tgaccggtaa agaagcagac gccgctctgg
    17761 gccgtgctaa catcaccgtc aacaaaaaca gcgtaccgaa cgatccgaag agcccgtttg
    17821 tgacctccgg tattcgcgtg ggtactccgg cgattacgcg tcgcggcttc aaagaagcag
    17881 aagcgaaaga actggctggc tggatgtgtg acgtgctgga cagcatcaat gatgaagccg
    17941 ttatcgagcg catcaaaggt aaagttctcg acatctgcgc acgtttcccg gtttacgcat
    18001 aagcgaaacg gtgatttgct gtcaatgtgc tcgttgttca tgccggatgc ggcgtgaacg
    18061 ccttatccgg cctacaaaac tttgcaaatt caatatattg caatatccgt gtaggcctga
    18121 taagcgtagc gcatcaggca atcttaggta tgtgatcatc aaggcttcct tcgggaagcc
    18181 tttctacgtt attgcgccat caaatctgtc gtaactgcgc ctcaacatac aaatagccaa
    18241 tccccaacac ctgttgtgcg cggcttaatt gcccaaagcc aatttgcgtt gctttcacgc
    18301 tcggcccttc gtcgcggtcg aacgggttaa atcccgtctg gcgaataatg gtattaagcc
    18361 agtgctcacc aaaggcacaa ctgcgaccgt acaaccaaat ctgattaata ttgaggatgt
    18421 tgaggaagtt atacagactc aaaccaatgg cattggcact gcgatcaacc caactggtga
    18481 tccacggttc tccactctac caggcagcga ttaactgcgc tgtagtcagt ttttccggat
    18541 caagctgagt attaaccggt tgtgatttta gccatacccg cgcctgtttt tttaatgcac
    18601 ttaacgaggc gacggtttcc aggcagccgt aacgtccaca gtcgcagacg acgccatccg
    18661 gattaacgat ggtgtgacca atctgtccgc taccatacaa actgccgcga taaatttgct
    18721 cgttaatcac gaacgacgag ccaatgccgt aatcaacgtt gatcacgcag aaatcccgtt
    18781 tctgcgaatt attttgccat ttctccgcca gcgccagcat cacgcagtca ttatcgacca
    18841 tcacccgaat gccgagcttc tcttccagca gatacttcac ctcaaccggc gttgtccacg
    18901 gcgcttgcgg catggtttgc gacacgccgg tcaccggatc aacctgaccg tggattgcca
    18961 gcgccagatt gatggtgcgg tccggccaca atttacggtg gcgatgccag catttttcga
    19021 tttcggacag cagcgcctgc ggcgtcggtg catcaatctg taaatactca aattcacctt
    19081 tcggacttaa acaagcatta gcaacctgac actcaatact ggtgggcgtc acgttcaggc
    19141 acagcgtcca gtcaccttcc ggcgcaatca gccatgtacc gctactatgc ccgcgcgtct
    19201 ggctttcatc gtcgatattc accacccgtt tttcgctttc cagttcctgc aaaatattac
    19261 tgactgccgg aatcgaaatt tgcgccagcc gggccagcgt tgatttattg gcgcgctttt
    19321 gccggtacag cagttccaga atcacgcatt tgttatggtg gcgaatctgt tgattgttaa
    19381 tgcaggctct catcttcact aaaccttgtt tagttaattg cgtgattatt ccgcgaaaac
    19441 attgccagat tgttcaatac actgccacaa atcttttaat tcagtatgtc ttgttaatat
    19501 tgagggcacc atgactccag taaaagtgtg gcaagagcgc gttgagatcc cgacctatga
    19561 aaccgggccg caggatatac atcccatgtt cctggaaaat cgcgtttatc agggatcgtc
    19621 cggcgcggtt tatccctacg gcgtgaccga tacgctgagc gagcagaaaa ccctgaaatc
    19681 ctggcaggcg gtgtggctgg aaaacgacta catcaaagtg atgatcctgc cggaactggg
    19741 cggtcgggtg catcgcgcgt gggataaagt gaaacagcgc gattttgttt atcacaatga
    19801 agtcattaaa cctgcgctgg tggggctgct ggggccgtgg atttccggtg ggattgagtt
    19861 taactggccg caacatcatc gcccgaccac ctttatgccc gttgatttca ccctcgaagc
    19921 ccatgaagac ggtgcacaga cggtgtgggt aggcgaaacg gagccgatgc atggtttaca
    19981 ggtgatgaca ggtttcaccc tgcgccctgg gcggcgctgg aaatcgccag ccgcgtctat
    20041 aacggcaacg ccacgccgcg tcatttcttg tggtgggcca acccggcagt gaaagggggt
    20101 gaagggcatc agagcgtttt cccaccggat ttgactgcgg tgtttgatca cggcaaacgg
    20161 gccgtctccg ctttccccat cgctaccggc acttactaca aagtggacta ctccgctgga
    20221 gtggacattt ctcgctataa aaatgtgccc gttccaacct catatatggc cgaaaaatcg
    20281 cagtacgatt ttgtcggcgc gtggtgtcac gatgaagatg gcggtttgct gcacgttgcc
    20341 aaccaccata ttgcgccagg taaaaaacag tggagttggg gacacagtga atttggccag
    20401 gcgtgggata agagtctgac cgacaataac ggcccgtata tcgaactgat gaccggtatt
    20461 tttgccgata accagcccga ttttacctgg cttgatgctt acgaagagaa gcgttttgag
    20521 cagtatttcc tgccttatca ttctctgggc atggtgcaaa atgcctcccg cgatgcggtg
    20581 ataaaactcc agcgtagtga gcaggggatt gagtgggggc tgtatgccat ctctccgttg
    20641 aacggatacc gcctggcgat ccacgaaatc ggcaaatgca acgcgttact tgatgatgcc
    20701 gtggcactga tgcctgcgac cgccatccag ggcgtgttgc acggtatcaa tcctgatagg
    20761 ctgaccattg agctttccga tgccgacggc aatattgtac tgagttatca ggaacatcag
    20821 ccgcaagcgt tgccgttacc ggacgtcgcc aaagcgccac tggcagcaca agacattacc
    20881 agtacagatg aagcctggtt tatcggtcag catctggagc aatatcatca cgccagccgt
    20941 tcaccgttcg attactacct gcgcggcgtg gcgctggacc cgctggatta tcgctgtaac
    21001 ctggcgctgg cgatgctgga atataaccgc gcagatttcc cgcaagcggt ggcgtatgcc
    21061 agtcaggcgc tgaaacgcgc acatgcgctg aacaaaaacc cgcagtgtgg acaagcaagt
    21121 ttgattcgcg ccagtgctta cgaacgtcag ggacaatatc aacaagccga agaggatttc
    21181 tggcgtgcgg tctggagcgg caacagcaaa gccggtggct attatggcct ggcacgactg
    21241 gcggcgcgta atggtaactt cgacgctggt ctggattttt gccaacaaag tcttcgcgcc
    21301 tgcccaacca atcaggaagt gctttgcctg cataatctgc tgctggtgtt aagtggtcgt
    21361 caggacaacg cgcgtttgca gcgcgcgaaa ctgctgcgtg attatccgct gaacgccatt
    21421 ctgtggtggc tgaactggtt cgatggtcgt agcgaatcag ccctcgcgca gtggcgcggt
    21481 ctgtgtcagg gacgcgacgt taacgccctg atgaccgccg ggcaactgat taactgggga
    21541 atgcccactc tggcgacaga gatgctgaac gcactggact gtcagcgcac gttatcgctt
    21601 tatctacaag ccagcttgct gccgaaagcc gaacgcggcg aactggtcgc aaaagccatt
    21661 gatgtcttcc cgcagtttgt ccgtttcccg aatacgctgg aagaagtggc ggcgctggag
    21721 agtattgaag agtgctggtt tgcccgtcat ttactggcct gcttctacta caacaagcgc
    21781 agctacaaca aagccattac tttatggcaa cgttgcgtag agatgtcgcc ggagtttgcc
    21841 gatggctggc gcgggttggc gatccatgcg tggaataagc aacacgatta tgaactggcc
    21901 gcgcgttatc ttgataatgc ttatcagctt gcgccgcagg atgcacgtct gcttttcgaa
    21961 cgggatttgc tggataagtt aagtggagcc acaccggaga aacgactggc gcgtctggaa
    22021 aataatctgg aaattgcgct gaaacgcgac gacatgaccg cagaactgct caatttgtgg
    22081 catctcacgg gtcaggcaga caaagcggcg gacattctcg ccacgcgcaa attccacccg
    22141 tgggaaggcg gggaagggaa ggtcaccagt cagtttatcc tcaaccagtt attacgcgcc
    22201 tggcagcatc ttgatgccag acagccgcag caggcctgcg aactgcttca tgccgcgctg
    22261 cattatccgg agaatttaag cgaaggccgt ttaccggggc aaactgataa cgacatctgg
    22321 ttctggcagg cgatatgcgc caacgcgcag ggcgatgaaa ctgaagcgac gcgttgttta
    22381 cgtctggcgg cgaccggcga tcgcaccatt aacatccaca gttattacaa cgatcagccg
    22441 gttgattatc tcttctggca aggaatggcg ctgcgactgc tgggcgaaca acaaatcgca
    22501 cagcaactgt ttagtgaaat gaaacagtgg gcgcaagaga tggcgaaaac cagtattgag
    22561 gcggatttct ttgctgtttc acaacctgac ctgttgtcgc tgtatggcga tttacaacag
    22621 cagcataaag aaaaatgcct gatggtggcg atgctggcgt ccgcgggact cggggaggtt
    22681 gcgcaatatg aatctgctcg cgctgaattg acggcgatta atccggcctg gccgaaagcg
    22741 gcattattca ccaccgtgat gccttttatt tttaactacg ttcactaaac cctattaact
    22801 gtaatatttg agcggcacgc gctgccgctc atcacaaaat aattagaata agccgttgtt
    22861 aataaaaact ctacaggagg cactatgcct aaaaaaatga gaacaacccg taatctatta
    22921 ttaatggcta cgctattagg tagcgcacta tttgccaggg ctgcggataa agaaatgacc
    22981 attggtgcaa tataccttga taccctggga tattacgctg gagtgcgtca gggcgttcag
    23041 gatgcggcaa aagattcttc agtacaggta cagttaattg aaactaacgc ccagggtgat
    23101 atttcgaaag aaagtacctt tgttgatacc ctcgtggcgc gtaatgtcga tgccattatt
    23161 ttatcggcag tgtctgaaaa tggcagtagc cgtaccgttc gtcgcgccag tgaagcgggt
    23221 attccggtga tttgctacaa cacctgtatt aatcaaaagg gtgtcgataa atatgtctcg
    23281 gcgtatctgg tcggcgatcc actggaattt ggtaaaaaac tgggtaacgc tgccgccgat
    23341 tattttattg ccaataaaat tgaccagccg aaaattgccg tcatcaattg cgaagccttt
    23401 gaagtttgtg tgcagcgacg taaaggattt gaagaagtat taaaatcccg cgttcccggc
    23461 gcgcaaattg tcgctaatca ggaagggact gttttaaata aagcgatttc cgttggtgaa
    23521 aaactgatta tctccacgcc ggatctcaac gccattatgg gggagtcggg cggtgcgaca
    23581 ctcggcgcgg taaaagcggt acgtaatcaa aatcaggccg gaaaaattgc tgttttcggt
    23641 tcggatatga caaccgaaat tgctcaggag ctggaaaaca atcaggtgct gaaagcggta
    23701 gtggatattt ccggtaagaa aatgggcaat gctgttttcg cgcaaacatt gaaggttatc
    23761 aataaacaag ccgacggtga aaaagtgatt caggtgccaa tcgatctcta taccaaaacg
    23821 gaagatggta aacagtggct ggcaacgcac gttgatggtc tgccctaatc gctgattcgc
    23881 agaggtccct atgttcacgg caacagaggc agtcccggta gcaaaagtgg tggcaggaaa
    23941 taagcgttac cccggcgtcg ttgcgctgga taacgttaac ttcacgctca ataaaggcga
    24001 agttcgtgcg ctgttaggta aaaacggcgc gggcaaatcg actctcattc gaatgcttac
    24061 cggcagcgaa cgcccggata gcggtgatat ctggattggc gagacgcgac tggaaggtga
    24121 cgaagctacg ttgactcgcc gtgccgctga actgggggtt cgtgcggttt atcaggaatt
    24181 aagtctggtg gaagggctga cggtggcaga aaacctctgc cttggtcaat ggcctcgccg
    24241 caacggcatg attgattacc tgcaaatggc gcaggatgcc caacgttgct tacaggcgct
    24301 gggcgttgac gttagtcctg aacaacttgt ttcaacgcta agcccggcgc aaaagcaact
    24361 ggtggaaatt gcacgggtga tgaagggcga gccgcgcgtg gtcattcttg atgaacccac
    24421 cagctcgctt gccagtgcag aagttgaact ggtgatcagt gcggtgaaaa agatgtcggc
    24481 actgggcgtg gcggtgattt atgtcagcca ccggatggaa gaaatccgcc gcattgcctc
    24541 ctgtgccacc gttatgcgcg atggtcaggt ggcgggcgat gtgatgctcg aaaacacctc
    24601 cacgcatcat attgtgtcgc taatgctagg gcgtgatcac gttgatattg cgccggttgc
    24661 ccctcaggaa attgtggatc aggccgtact ggaagtccgt gcgttacgcc ataagcccaa
    24721 gctggaggat atcagcttta cgttacgtcg cggcgaagtg cttggcattg ctggcctgct
    24781 gggggcaggg cgcagtgaat tgctgaaggc gattgttggg ctggagacgt atgaacaggg
    24841 cgaaattgtt atcaacggcg agaaaatcac gcgccccgat tacggcgaca tgctgaaacg
    24901 cggcattggc tatacgccag aaaaccgcaa agaagcgggg atcattccct ggttgggcgt
    24961 tgacgaaaat acagtgctga ccaatcggca aaaaatcagc gccaacggag tgctgcaatg
    25021 gtccaccatc cgccgcctga ccgaagaggt gatgcagcgg atgacggtca aggccgctag
    25081 tagcgaaacg cccatcggca cgctttctgg tggcaatcag caaaaagtgg tgatcggtcg
    25141 ttgggtctat gccgccagcc agattttgtt gctcgacgag ccaacgcgcg gcgtcgatat
    25201 cgaagccaaa cagcagattt accgtattgt ccgtgagctg gctgccgaag gaaaaagcgt
    25261 ggtgtttatc tccagtgaag tggaggagtt gccgctggtg tgtgaccgca tcctgttatt
    25321 acagcacggc acgttctcgc aggagtttca ctcgccggtc aatgtggatg agctgatgtc
    25381 cgccattctg tctgtgcact gatgaatttt accgaggaac ctgaagatgt ctgcttcgtc
    25441 attaccattg ccgcagggca agagcgtctc gctcaaacaa tttgtcagtc gccatattaa
    25501 tgagatcggt ttgctggtgg tgattgccat tctttatctg gtcttctccc tgaacgcgcc
    25561 tggctttatc tcattgaata accagatgaa cgtgctgcgc gatgccgcca ccattgggat
    25621 tgccgcctgg gcgatgacgc tgattattat ctccggtgaa attgatgtca gcgttgggcc
    25681 gatggtggct tttgtctcgg tgtgcctggc atttttgctg caatttgaag ttccgttggc
    25741 gattgcttgt ctgctggtgt tgctgttagg cgcgctgatg ggaacgctcg ccggggtgct
    25801 gcgcggcgtg tttaacgtac caagtttcgt tgccacactg gggctgtgga gcgccctgcg
    25861 cggaatgggg ctgtttatga ccaacgcact gccagtgccg attgacgaaa acgaggtgct
    25921 ggactggctg ggcggacaat ttctcggtgt gccagtatcc gcgctgatca tgatggtgtt
    25981 gtttgcgctg tttgtgttca ttagccgaaa aaccgccttc gggcgctcgg tttttgccgt
    26041 tggcggtaat gccacggcgg cgcagttgtg cggtattaac gttcgtcggg tgcgcattct
    26101 tatctttacc ctttcgggat tattagcggc ggtgaccggc attttgctgg cggcacgcct
    26161 cggttccggt aacgcaggtg ccgcaaacgg tctggagttt gacgtcatcg ccgcggtcgt
    26221 ggtcggcggt acggcacttt ccggcggtcg cggttccctg ttcggtacat tgcttggtgt
    26281 gctggtgatt acgctaatcg gtaacggtct ggtgctgctc ggtattaact cctttttcca
    26341 gcaggtggta cgcggcgtca tcatcgtggt ggcggtgctg gcgaatatct tgctgaccca
    26401 gcgaagcagt aaagcgaaac gctaaacctt aacgcccctg gcgcgggtgc ggcagggatg
    26461 aaaattaatc gtattgttaa tgagggattt atgaaaacga tgctggcagc ttatttacca
    26521 ggaaattcaa ccgtcgatct gcgggaagtt gcggtgccga cgccggggat taaccaggta
    26581 ctgatcaaaa tgaaatcctc cgggatttgc ggaagcgatg tccactatat ctatcatcaa
    26641 cactgtgcca cggcggcggc acccgataaa ccgttatacc agggatttat caacggtcat
    26701 gaaccgtgcg ggcagattgt ggcgatgggg caaggctgcc gccattttaa agagggcgac
    26761 cgcgtgctgg tgtatcacat ttctggctgt ggtttttgcc cgaactgccg ccgcggcttt
    26821 cctatctctt gtactggcaa aggaaaagcg gcttacggct ggcagcgtga cggcggtcat
    26881 gccgaatatc tgctggcgga agaaaaagat ctgatcctcc tgccggatgc gctgagctac
    26941 gaagatggtg cgtttatcag ttgcggcgtt ggcacggcct atgaagggat tttgcgcggc
    27001 gaagtttccg gcagcgataa cgtgctggtg gtcggtctgg ggccggtcgg catgatggcg
    27061 atgatgctgg cgaaaggtcg cggtgcaaaa cggatcatcg gcgttgatat gctgccggaa
    27121 cgtctggcga tggcaaaaca gttaggggtg atggatcacg gctatttagc aaccaccgaa
    27181 ggtctgccgc agattatcgc cgaactcacc cacggtggcg cggatgttgc gctcgattgt
    27241 tctggtaatg ccgcaggtcg cttgctggca ctgcaatcca ccgctgactg gggacgggtg
    27301 gtttacattg gtgaaaccgg aaaagtggaa ttcgaggtca gcgccgatct gatgcaccat
    27361 caacggcgga ttatcggctc ctgggtgacc agtctgttcc atatggaaaa atgcgcccac
    27421 gatttgactg actggaagct gtggccgcgt aacgccatta cccatcgctt ctcgctggaa
    27481 caggcaggtg atgcctatgc gctgatggcg agcggcaaat gcgggaaagt tgtgattaac
    27541 ttcccggatt aaggagttag cggatgacca tctatacctt atcccacggg tcgctaaagc
    27601 tggacgtttc tgaccagggc ggcgtaatcg aaggcttctg gcgcgatacg acgccgctct
    27661 tacgtcctgg taaaaaaagc ggtgtggcaa ccgatgcctc atgttttccg ctggtgccat
    27721 ttgcaaaccg ggtgagcggc aaccgttttg tctggcaggg gcgtgagtat caactgcaac
    27781 cgaatgttga gtgggatgcg cactatctac acggcgatgg ctggctgggc gagtggcaat
    27841 gtgtctcgca cagcgatgat agtttgtgtc tggtgtatga acatcgcagc ggtgtctatc
    27901 actatcgggt aagtcaggcg tttcatttaa cggcggatac gctgacggtg acgctctctg
    27961 tcaccaatca aggggcagag acgctgccat ttggtaccgg ctggcatcct tattttccgt
    28021 tgtcaccaca aacgcggatt caggcgcagg cgagcggtta ctggctggaa cgggagcagt
    28081 ggctggcggg tgagttttgc gagcagctac cgcaggaact ggattttaac cagctcgcgc
    28141 cgttgccgcg ccagtgggtg aacaatggtt ttgccggatg gaatggtcag gcccgcatcg
    28201 agcagccgca ggaaggttat gccatcatca tggaaacgac gccacctgca ccgtgttatt
    28261 tcatctttgt ttccgaccct gcgtttgata aaggatatgc gtttgatttc ttctgtctgg
    28321 aaccgatgag ccatgcgccg gatgatcatc atcgcccgga agggggtgat ctcattgcgc
    28381 tagcaccagg ggaatcaaca acttcagaga tgtcgttgcg ggtagagtgg ctgtaattta
    28441 ttaaatgccg gataaggcgt tcacgccgca tccggcatct ttcctaacgc cgatcgagag
    28501 aaattgcccc cggcccggta attgccaata gcaagaacgc gccagcgata ctgacattct
    28561 tccagaaatt aatcatattt ggcccaaccg catcgccggt catatcccag taatggtgac
    28621 caatcaccgc cgtacccagc gtgtagaaaa taaacagcac cgccagcgga cgggtgaaaa
    28681 agccaagcac gattaatatc gcggcgggca cttccataac taccgcaata atcgctgcca
    28741 gcatcggcat tggcgcgccc aacgaggcca tatattggac cgtaccgtca aagcccatca
    28801 ttttgggaaa accaaaaata atgaaaatta agaccacggc gatacgggca attaataaca
    28861 aaacggggcg ggcagctcca aaatcaaaat aac
//
LOCUS       DAZPLI010000037        28632 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZPLI010000037 DAZPLI010000000
VERSION     DAZPLI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28632
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.037"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..72
                     /locus_tag="ACHUU3_001580"
     CDS             <1..72
                     /locus_tag="ACHUU3_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2081681.1"
                     /translation="ARRRCDVLFAMMRDGTFYTPQGS"
     gene            481..930
                     /gene="alaE"
                     /locus_tag="ACHUU3_001581"
     CDS             481..930
                     /gene="alaE"
                     /locus_tag="ACHUU3_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR2081682.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
     gene            complement(967..1311)
                     /locus_tag="ACHUU3_001582"
     CDS             complement(967..1311)
                     /locus_tag="ACHUU3_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR2081683.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1463..1792
                     /locus_tag="ACHUU3_001583"
     CDS             1463..1792
                     /locus_tag="ACHUU3_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFR2081684.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            2040..2285
                     /gene="nrdH"
                     /locus_tag="ACHUU3_001584"
     CDS             2040..2285
                     /gene="nrdH"
                     /locus_tag="ACHUU3_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR2081685.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2282..2692
                     /gene="nrdI"
                     /locus_tag="ACHUU3_001585"
                     /pseudo
     CDS             2282..2692
                     /gene="nrdI"
                     /locus_tag="ACHUU3_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            2665..4809
                     /gene="nrdE"
                     /locus_tag="ACHUU3_001586"
     CDS             2665..4809
                     /gene="nrdE"
                     /locus_tag="ACHUU3_001586"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR2081686.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4819..5778
                     /gene="nrdF"
                     /locus_tag="ACHUU3_001587"
     CDS             4819..5778
                     /gene="nrdF"
                     /locus_tag="ACHUU3_001587"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR2081687.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            6134..7336
                     /gene="proV"
                     /locus_tag="ACHUU3_001588"
     CDS             6134..7336
                     /gene="proV"
                     /locus_tag="ACHUU3_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR2081688.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            7329..8393
                     /gene="proW"
                     /locus_tag="ACHUU3_001589"
     CDS             7329..8393
                     /gene="proW"
                     /locus_tag="ACHUU3_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR2081689.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8451..9443
                     /gene="proX"
                     /locus_tag="ACHUU3_001590"
     CDS             8451..9443
                     /gene="proX"
                     /locus_tag="ACHUU3_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR2081690.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9634..10818
                     /locus_tag="ACHUU3_001591"
     CDS             9634..10818
                     /locus_tag="ACHUU3_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2081691.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            10942..11679
                     /gene="ygaZ"
                     /locus_tag="ACHUU3_001592"
     CDS             10942..11679
                     /gene="ygaZ"
                     /locus_tag="ACHUU3_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR2081692.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11669..12004
                     /gene="ygaH"
                     /locus_tag="ACHUU3_001593"
     CDS             11669..12004
                     /gene="ygaH"
                     /locus_tag="ACHUU3_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR2081693.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            12095..12625
                     /gene="emrR"
                     /locus_tag="ACHUU3_001594"
     CDS             12095..12625
                     /gene="emrR"
                     /locus_tag="ACHUU3_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR2081694.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12752..13924
                     /gene="emrA"
                     /locus_tag="ACHUU3_001595"
     CDS             12752..13924
                     /gene="emrA"
                     /locus_tag="ACHUU3_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFR2081695.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYCFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            13941..15479
                     /gene="emrB"
                     /locus_tag="ACHUU3_001596"
     CDS             13941..15479
                     /gene="emrB"
                     /locus_tag="ACHUU3_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR2081696.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15543..16058)
                     /gene="luxS"
                     /locus_tag="ACHUU3_001597"
     CDS             complement(15543..16058)
                     /gene="luxS"
                     /locus_tag="ACHUU3_001597"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR2081697.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(16208..17764)
                     /gene="gshA"
                     /locus_tag="ACHUU3_001598"
     CDS             complement(16208..17764)
                     /gene="gshA"
                     /locus_tag="ACHUU3_001598"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR2081698.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            complement(17837..18265)
                     /locus_tag="ACHUU3_001599"
     CDS             complement(17837..18265)
                     /locus_tag="ACHUU3_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR2081699.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(18262..18828)
                     /gene="yqaB"
                     /locus_tag="ACHUU3_001600"
     CDS             complement(18262..18828)
                     /gene="yqaB"
                     /locus_tag="ACHUU3_001600"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR2081700.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSIEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(19111..19187)
                     /locus_tag="ACHUU3_001601"
     tRNA            complement(19111..19187)
                     /locus_tag="ACHUU3_001601"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19151..19153),aa:Arg,seq:acg)
     gene            complement(19386..19462)
                     /locus_tag="ACHUU3_001602"
     tRNA            complement(19386..19462)
                     /locus_tag="ACHUU3_001602"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19426..19428),aa:Arg,seq:acg)
     gene            complement(19526..19602)
                     /locus_tag="ACHUU3_001603"
     tRNA            complement(19526..19602)
                     /locus_tag="ACHUU3_001603"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19566..19568),aa:Arg,seq:acg)
     gene            complement(19606..19698)
                     /locus_tag="ACHUU3_001604"
     tRNA            complement(19606..19698)
                     /locus_tag="ACHUU3_001604"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19662..19664),aa:Ser,seq:gct)
     gene            complement(20014..20199)
                     /gene="csrA"
                     /locus_tag="ACHUU3_001605"
     CDS             complement(20014..20199)
                     /gene="csrA"
                     /locus_tag="ACHUU3_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR2081701.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(20434..23064)
                     /gene="alaS"
                     /locus_tag="ACHUU3_001606"
     CDS             complement(20434..23064)
                     /gene="alaS"
                     /locus_tag="ACHUU3_001606"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR2081702.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(23192..23692)
                     /gene="recX"
                     /locus_tag="ACHUU3_001607"
     CDS             complement(23192..23692)
                     /gene="recX"
                     /locus_tag="ACHUU3_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR2081703.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(23920..24981)
                     /gene="recA"
                     /locus_tag="ACHUU3_001608"
     CDS             complement(23920..24981)
                     /gene="recA"
                     /locus_tag="ACHUU3_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR2081704.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(25061..25558)
                     /gene="pncC"
                     /locus_tag="ACHUU3_001609"
     CDS             complement(25061..25558)
                     /gene="pncC"
                     /locus_tag="ACHUU3_001609"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR2081705.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(25703..26788)
                     /gene="mltB"
                     /locus_tag="ACHUU3_001610"
     CDS             complement(25703..26788)
                     /gene="mltB"
                     /locus_tag="ACHUU3_001610"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR2081706.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            27044..27607
                     /gene="srlA"
                     /locus_tag="ACHUU3_001611"
                     /gene_synonym="gutA"
     CDS             27044..27607
                     /gene="srlA"
                     /locus_tag="ACHUU3_001611"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFR2081707.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            27604..28563
                     /gene="srlE"
                     /locus_tag="ACHUU3_001612"
                     /gene_synonym="gutE"
     CDS             27604..28563
                     /gene="srlE"
                     /locus_tag="ACHUU3_001612"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR2081708.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
BASE COUNT         6753 a         7571 c         7144 g         7164 t
ORIGIN      
        1 gcgagacgac gctgcgacgt tctgttcgcc atgatgcgcg acgggacttt ttataccccg
       61 caggggtcat aacatgcttg acaacttaat aggggcaccc ccctcactgg tgctttctgc
      121 tgttaatgta cattttctgg catcaaaaat gctgtaaatg acacggtcac gacatacacc
      181 gaattctcta cgcttatagc gttacctcac ccccaaactt tcattagtga aattaaaggc
      241 aaggtcatat ctaattgatt taattgaaaa taaatcatat cggcatgtaa aaatccacaa
      301 tgtacaaaaa acgagccgtt acggaatatt ttatctacaa aaactgacta aataaaaatt
      361 tttcactaat tgaatagtca tagccagcga tatacgctat gcgaaaatgc agatggcaat
      421 gagatccact gctttcatct ccattaacat cccattacgc ttttattaag gagcattagc
      481 atgttctcac cgcagtcacg cttgcgtcat gcagttgcag atacgttcgc gatggttgtt
      541 tactgttctg tcgtgaacat gtgtattgaa gttttcctct ccggaatgag cttcgaacag
      601 tctttttatt ccagattggt agcgattccg gtgaacatct taattgcatg gccatacggt
      661 atgtaccgcg atctatttat gcgcgcggca cgcaaagtta gcccgtcggg ctggataaaa
      721 aatctggctg atatcctggc ttatgtgacg ttccagtcac cggtgtatgt ggcgatcttg
      781 ttagtggtgg gcgcagactg gcatcagatt atggcggcgg tcagttcaaa catcgttgtt
      841 tcgatgttga tgggggcggt ttatggctac ttcctcgatt attgccgccg actgtttaaa
      901 gtcagccatt accagcaggt aaaagcctga ctcttctttt cgcgactggc atcgccagtc
      961 gcgtctttaa ctggcttcgc caaataaacc attcaaataa cgttcaagcg caacacgcga
     1021 actgaagcca tgcgggatac cgtaatgctc gtgtcgctcg cctgctaaat agagcggaaa
     1081 ttgttgataa tgatcgcaag ttttaatatc ggaagcgggt tcagccagtg tcgaactacg
     1141 ttcttttaat gttggatgaa ttaccagtgc ggtacgtccc attcgcgctt cgcgattaac
     1201 atagacataa ttttccccac ggcggtaacc ataagctttc tgggttacca catcgacagt
     1261 aaaaccgact ttttcaagta cgcgcgccac ctcgtctggt cgtaaataca tatttgatcc
     1321 tcgttattat caaccgcggg cctaccttac ctgattgcgc attcacaacg ctttcagaaa
     1381 agtccataaa gccgcgatga acctcagtta agaaaattat ggtctacact gaaaattaca
     1441 tcgaattcta atgggggatc atatgtttaa ccgaccgaac cgcaacgacg ttgatgatgg
     1501 cgtgcaggat atccagaatg atgtcaatca attagctgac agcctcgaat ctgtattgaa
     1561 gtcctggggc agcgacgcca aaggggaagc tgaagccgca cgcagtaaag ctcaggcatt
     1621 gctgaaagaa accagggcgc gaatgcatgg tcgtactcgc gtccagcaag ccgcacgcga
     1681 tgccgttggc tgcgcggatt cttttgttcg tgaaagaccc tggtgtagcg tgggtacagc
     1741 agctgcggta ggtattttta tcggcgcact gttaagcatg cgcaaatcgt agtgcaaaaa
     1801 tgataataaa tacgcgtctt taaccccgaa gcctgtcttc ggggtttctt tttgcctggt
     1861 gaatcacaaa aatccccctc ccccgtcacg ctcatatcta gggtaatttc gatcactatt
     1921 tgctatatat tgtgtggttg aatctttttt caactacatc tagtatctct gtatcaacag
     1981 agagacaacc cgacgcgtat catcgcgccg tatcttcatt ttaaacggaa atacgaatca
     2041 tgcgcataac tatttacact cgtaacgatt gcgttcagtg ccacgccacc aaacgggcga
     2101 tggaaaaccg gggctttgat tttgaaatga ttaatgtcga tcgcgttcct gaagcagcgg
     2161 aagcgttgcg tgctcagggc tttcgtcagt tgccggtagt gattgctggc gatcttagct
     2221 ggtctggttt ccgtccggac atgattaacc gtctgcatcc agcgccacac gcggccagtg
     2281 catgagccag ctcgtctact tctccagcag ctccgaaaac acgcagcgtt ttatcgaacg
     2341 tttaggtctg cccgcggtgc gcatcccgct caatgagcgg gaacggattc aggtagacga
     2401 gccttacatc ctgatcgtgc cctcttacgg cggcggcggt acggctggcg cggtgccacg
     2461 ataggtaatt cgctttttaa acgacgagca caaccgggcg ttgcttcgcg gcgttattgc
     2521 ttctggtaat cgcaactttg gtgaggcgta tggccgcgcc ggagatgtga ttgcccggaa
     2581 atgcggcgtg ccgtggctgt accgttttga actcatgggt acgcaaagcg atatcgaaaa
     2641 cgttcgtaaa ggagtaaccg aattttggca acgacaaccg cagaatgcct gacgcaggaa
     2701 acgatggatt accacgcgct gaatgcgatg cttaacctct acgatagcgc aggtcgcatt
     2761 cagttcgata aagaccgcca ggccgttgac gcctttatgg cgacgcatgt gcgtccgaac
     2821 agtgtgacct tcagtagcca gcagcagcgc ctgaactggc tggtcaacga aggttactat
     2881 gatgaaagcg ttcttaatcg ctactctcgc gactttgtca ttacgctgtt tgcccacgca
     2941 cacaccagcg gttttcgttt ccagacattc ctcggggcat ggaagtttta caccagctat
     3001 acgttgaaga cattcgacgg taaacgttat ctggaagatt ttgccgatcg agtcacgatg
     3061 gtggcgctga cgctggcaca aggcgatgcg acgctggcgt tgcaactgac cgatgaaatg
     3121 ctgtcaggac gctttcagcc agccacgccg acattcctca actgcggtaa gcagcagcgc
     3181 ggcgaactgg tttcctgttt tctgctgcgt attgaagaca atatggagtc gattggccgg
     3241 gcggtaaatt ccgcactgca actgtcgaaa cgcggcggcg gcgtggcatt tttgctgtcg
     3301 aatctgcgag aagcgggcgc gccaattaaa cgtattgaaa atcaatcttc tggcgtaatt
     3361 ccggtgatga aaatgctgga agacgcattt tcctatgcca accaactcgg cgcgcgtcag
     3421 ggggcaggtg cagtctattt acatgctcat catcccgata ttctgcgttt tctcgataca
     3481 aaacgggaaa atgccgacga aaaaatccgc attaaaacac tgtcgctggg cgtggtgatc
     3541 ccggatatca ctttccatct ggcaaaagag aatgcgcaga tggcgctgtt ttcgccctat
     3601 gacgttgagc gagtttatgg caagccgttt gccgatatcg ccatcagcga acactatgac
     3661 gaactggttg ccgatgaacg catccgcaaa aaatacctca acgcccgtga tttcttccag
     3721 cggctggcag aaatccagtt tgagtccggc tatccctaca tcatgtatga agacacggta
     3781 aaccgtgcta accctgtcgc cgggcgcata aatatgagta atctctgctc agagattttg
     3841 caggtcaact gtgcctcaga gtatgacgag aatctcgact atgcccgcac aggccatgat
     3901 atctcctgca atttaggttc gttgaatatt gcgcacacca tggattcccc cgattttgcc
     3961 cgcacggtag agactgccgt gcgcggttta acggcggtgt cagatatgag tcatatccgc
     4021 agcgtgccgt ccatcgaagc cggaaatgcc gcctcacacg ccatcggact ggggcagatg
     4081 aatttacacg gctatctggc gcgagaaggc atcgcttatg gttcgccgga agcactggat
     4141 ttcaccaatc tctatttcta tgccatcacc tggcacgcac tgcgtacctc gatgttgctg
     4201 gcgcgcgaac gcggtgaaac cttcgccggg ttcaaacagt cacgttatgc cagtggtgaa
     4261 tattttagcc aatatctgca aggtaactgg cagccgaaaa cggcgaaagt tggcgaactg
     4321 tttgcccgca gcggtattac gttacctacc cgtgagatgt gggcgcaact gcgcgacgac
     4381 gtgatgcgct acggcattta caaccagaat cttcaggcgg tgccgccaac cggttctatc
     4441 tcttatatca accatgctac gtcgagtatt catccgattg tggcgaaagt agagatacgc
     4501 aaagagggca aaacaggacg cgtttactac cctgccccgt ttatgactaa cgaaaatctg
     4561 gcgctgtatc aggatgctta cgaaatcggc gcagaaaaga tcatcgacac ctacgcggaa
     4621 gcgactcgcc atgtcgatca gggtttgtcg ctgacgctgt ttttccccga taccgccacc
     4681 actcgcgata tcaacaaagc gcagatttac gcctggcgca aaggtatcaa aacgctctat
     4741 tacattcgcc tgcgtcagat ggcgctggaa ggcactgaaa ttgaaggctg cgtctcctgt
     4801 gcactttaag gaatatctat gaaactctca cgtatcagcg ccatcaactg gaacaagata
     4861 tctgacgata aagatctgga ggtgtggaat cgcctgacca gcaatttctg gctaccagaa
     4921 aaggtgccgc tgtcgaacga tattcctgcc tggcagacat taactgtcgt agaacaacaa
     4981 ctgacgatgc gcgtttttac tggcctgacg ctgctcgaca cgctgcaaaa tgttatcggc
     5041 gcgccttcgc tgatgcccga tgcactcacg cctcatgaag aagcggtatt atcgaatatc
     5101 agctttatgg aagcggttca tgcccgctct tacagttcga ttttctcgac gctgtgccag
     5161 accaaagatg tcgatgccgc ctacgcctgg agtgaagaaa acgcaccgtt gcagcgaaaa
     5221 gctcagatta ttcagcaaca ttatcgcggt gatgatccgc tgaaaaagaa aatcgccagt
     5281 gtgtttcttg aatctttttt gttctattcc ggtttctggc tgccgatgta tttttccagc
     5341 cgcggaaagc tgaccaatac cgcagacctg atccgtctga ttatccgtga tgaagcagtc
     5401 cacggttact acataggcta taaatatcag aaaaacatgg aaaagatatc tctgggacaa
     5461 cgtgaagagt tgaagagttt cgccttcgat ttgttgctgg aactctacga caacgagctg
     5521 caatacaccg atgagctgta cgccgaaacc ccgtgggctg acgatgtgaa agcgtttctc
     5581 tgttacaacg ccaataaggc tttgatgaat ctgggctacg aacctttatt tcccgcagaa
     5641 atggcggaag tgaatccggc aatcctcgcc gcgctttcgc cgaatgccga tgaaaatcac
     5701 gatttctttt ccggttcagg ctcctcttat gtgatgggga aagcggttga aacagaagat
     5761 gaagactgga atttctgagg gtgttatttt caaaaatatc actacccgcg acagggaaat
     5821 aattcccgcc aaatagcttt ttatcacgca aatattttgt ggtgatctac actgatactc
     5881 tgttgcatta ttcgcctgaa accacaatat tcaggcgttt tttcgctatc tttgacaaaa
     5941 aatatcaact tttctcgatt tgctctcagc ccttatatca cgggaaattc cggcgatttg
     6001 ctcgcatcaa tattcatgcc acatttgcca tcaggggttg cctcagattc tcagtatgtt
     6061 agggtagaaa aaagtaacta tttccattgg gtaatatatc gacataaaca aataacagga
     6121 atctttctat tgcatggcaa ttaaattaga aattaaaaac ctttataaaa tatttggtga
     6181 gcatccacag cgagcattca aatatatcga acaaggactt tcaaaagaac aaattctgga
     6241 aaaaactggg ctatcgcttg gcgtaaaaga cgccagtctg gccattgaag aaggcgagat
     6301 atttgtcatc atgggattat ccggctcggg taaatccaca atggtacgcc ttctcaatcg
     6361 cctgattgaa cccacccgcg ggcaagtgct gattgatggt gtggatattg ccaaaatatc
     6421 cgatgccaaa ctccgtgagg tgcgcagaaa aaagattgcg atggtcttcc agtcctttgc
     6481 cttaatgccg catatgaccg tgctggacaa tactgcgttc ggtatggaat tggccggaat
     6541 taatgccgaa gaacgccggg aaaaagccct tgatgcactg cgtcaggtcg ggctggaaaa
     6601 ttatgcccac agctacccgg atgaactctc tggcgggatg cgtcaacgtg tgggattagc
     6661 ccgcgcgtta gcgattaatc cggatatttt attaatggac gaagccttct cggcgctcga
     6721 tccattaatt cgcaccgaga tgcaggatga gctggtaaaa ttacaggcga aacatcagcg
     6781 caccattgtc tttatttccc acgatctcga tgaagccatg cgtattggcg accgaattgc
     6841 cattatgcaa aatggtgaag tggtacaggt cggcacaccg gatgaaattc tcaataatcc
     6901 ggcgaatgat tatgtccgta ccttcttccg tggcgttgat attagtcagg tattcagtgc
     6961 gaaagatatt gcccgccgga caccgaatgg cttaattcgt aaaacccctg gcttcggccc
     7021 acgttcggca ctgaaattat tgcaggatga agatcgcgaa tatggctacg ttatcgaacg
     7081 cggtaataag tttgtcggcg cagtctccat cgattcgctt aaaaccgcgt taacgcagca
     7141 gcaaggtctt gatgcggcgc tgattgatgc gccgttagca gtcgatgcac aaacgcctct
     7201 tagcgagttg ctctctcatg tcggacaggc accctgtgcg gtgcccgtgg tcgacgagga
     7261 ccaacagtat gtcggcatca tttcgaaagg aatgctgctg cgcgctttag atcgtgaggg
     7321 ggtaaataat ggctgatcaa aataatccgt gggataccac gccagcggcg gacagtgccg
     7381 cgcaatccgc agacgcctgg ggtacaccga cgactgcacc gactgacggc ggtggtgctg
     7441 actggctgac cagtacgcct gcgccaaacg tcgagcattt taatattctc gatccgttcc
     7501 ataaaacgct gatcccgctc gacagttggg tcactgaagg gatcgactgg gttgttaccc
     7561 atttccgtcc cgtcttccag ggcgtgcgcg ttccggttga ttatatcctc aacagtttcc
     7621 agcaattgct gctgggtatg ccagcgccag tggcgattat cgttttcgct ctcatcgcct
     7681 ggcagatttc cggggtcgga atgggcgtgg cgacgctggt ttcgctgatt gccatcggcg
     7741 caatcggtgc ctggtcgcag gcaatggtga ctctggcgct ggtgttaacc gccctgctgt
     7801 tctgtatcgt catcggtttg ccgttgggga tatggctggc gagaagtccg cgagcggcga
     7861 aaattattcg tccactgctt gatgccatgc agaccacgcc agcgtttgtt tatctggtac
     7921 caatcgtcat gctgtttggt atcggtaacg tgccgggcgt agtggtgacg atcatctttg
     7981 ctctgccgcc gattatccgt ctgacgattc tggggattaa ccaggttccg gcggatctga
     8041 ttgaagcctc gcgctcattc ggtgccagcc cgcgccagat gctgttcaaa gttcagttac
     8101 cgctggcgat gccgaccatt atggcgggcg ttaaccagac gctgatgctg gctctttcta
     8161 tggtggtcat cgcctcgatg attgccgtcg gcgggctggg tcagatggta cttcgcggta
     8221 tcggtcgtct ggatatgggc cttgccaccg ttggcggcgt cgggattgtg atcctcgcca
     8281 ttatcctcga ccgcctgacg caggccgttg ggcgcgactc acgcagtcgc ggcaaccgtc
     8341 gctggtacac cactggccct gtcggtctgc tgacccgccc attcattaag taactctgca
     8401 cttgcccggt aacgccgggc attatcaccc tgccaaaaaa aggaataaca atgcgacata
     8461 gcgtactttt tgcgacagcg tttgccacgc ttatctctac acaaactttt gcagccgatc
     8521 tgccgggcaa aggcattact gttaatccag ttcagagcac catcactgaa gaaaccttcc
     8581 agacgctgct ggtcagtcgt gcactggaga aattaggtta taccgtcaat aaacccagcg
     8641 aagtggatta caacgttggc tacacctcac ttgcttccgg cgatgcaacc ttcaccgccg
     8701 tgaactggac gccgctgcat gacaacatgt acgaagctgc cggtggcgat aagaaatttt
     8761 attgtgaagg ggtattcgtt aacggcgcgg cacagggtta cctgatcgat aagaaaaccg
     8821 ccgaccagta caaaatcacc aacatcgcac aactgaaaga tccgaagatc gccaaactgt
     8881 tcgataccaa cggcgacggt aaagcggatt taaccggctg taacccaggc tggggctgcg
     8941 aaggtgcgat caaccaccag cttgccgcgt atgaactgac caacaccgtg acgcataatc
     9001 aggggaacta cgcagcgatg atggccgaca ccatcagtcg ctacaaagag ggcaaaccgg
     9061 tgttttatta cacctggacg ccgtactggg tgagtaatga gctgaagcca gggaaagatg
     9121 tggtctggtt gcaggtgccg ttctccgcac tgccgggcga taaaaacgcc gataccaaac
     9181 tgccgaatgg tgcgaattat ggcttcccgg tcagcaccat gcatatcgtt gccaacaaag
     9241 cctgggccga gaaaaacccg gcagcagcga aactttttgc cattatgcag ttgccagtgg
     9301 cagatattaa cgcccagaac gccattatgc atgacggcaa agcctcagaa ggcgatattc
     9361 agggccatgt tgatggttgg atcaaagccc accagcagca gttcgatggc tgggtgaatg
     9421 aggcgctggc agcgcagaag taatttttat tcgggcggat aagtcgtttt tgcctcatcc
     9481 gccattctgt gcacaatgcc tgatgcgacg ctggcgcgtc ttatcatgcc tacaaaccac
     9541 atcgcacatt tcaacaagct atctttcgcc cccattcatc aacatccgct attattgatt
     9601 tccagcttaa tcatcacctg atgaacaaaa ataatgacta aacctaatca tgagcttagc
     9661 ccggcgctga tcgtgctgat gtctatcgcc accggtcagg cggtagccag taactattac
     9721 gcccagccat tgctcgacac catcgcgcgt aacttttccc tttccgccag ttcggcaggc
     9781 tttattgtta ccgccgcgca gttgggctat gcagcaggtc tgctgtttct tgttcccctc
     9841 ggtgatatgt ttgaacgccg ccgcctgatt gtctcgatga ccttactggc ggcaggcggc
     9901 atgttaatca ctgctagcag tcagtcgctg gcgatgatga tcctcggtac ggcattaacc
     9961 ggtttattct cagtcgtggc acaaattctg gttccgctgg cagcgacgct ggcttcaccg
    10021 gataaacgcg gcaaagtggt tggcaccatt atgagcggtc tgctgttggg gatcttgctg
    10081 gcacgaaccg ttgccgggtt actggcaaac ctcggcggct ggcgcaccgt cttttgggtt
    10141 gcctcggtgt taatggcact gatggcgcta gcattatggc gtggtctgcc acaaatgaaa
    10201 tcagaaaccc acctcaacta cccacagttg ttaggttccg ttttcagcat gtttatcagc
    10261 gataagatcc tccgcacccg cgcattgctg ggctgcctga cctttgccaa cttcagcatt
    10321 ctctggacct caatggcctt tttgcttgcc gctccacctt ttaactacag cgatggtgtc
    10381 attggtctgt ttggacttgc gggagctgcc ggagcgttgg gcgctcgtcc ggcgggcggt
    10441 tttgccgata agggtaaatc gcaccacacc acaactttcg gtctgctgct gctattactt
    10501 tcatggctgg cgatctggtt tggtcacact tccgtactgg cgctgattat cggcatcctg
    10561 gtgctggatc tcaccgtaca gggcgtgcat atcactaacc agacggtaat ttatcgaata
    10621 caccctgatg cgcgtaatcg cctgaccgca ggttacatga ccagctactt tattggcggt
    10681 gccgccggtt cgctaatttc agcctcagcc tggcaacatg gcggttgggc tggcgtttgt
    10741 ctggctggcg cgacgattgc cctagttaac ttactggtct ggtggcgagg ttttcatcgt
    10801 caggaagccg caaattaagc aatcagcaac cgttttcgtg gctttacact tataagggtg
    10861 ttaagaagcc catcagtctg ataaggttaa gatattcatt cagtctattt ataatattaa
    10921 caatcgttaa gcgtacactc tatggaaagc cctactccac agcctgctcc tggttcggcg
    10981 accttcatgg aaggatgcaa agacagttta ccgattgtta ttagttatat tccggtggcc
    11041 tttgcgttcg gtctgaatgc gacccgtctg ggattctctc ctctcgaaag cgtttttttc
    11101 tcctgcatca tttatgcagg cgcgagccag ttcgtcatta ccgcgatgct ggcagccggg
    11161 agtagtttgt gggttgctgc actgaccgtc atggcaatgg atgttcgcca tgtgttgtat
    11221 ggcccgtcac tgcgtagccg tattattcag cgtctgcaaa aatcgaaaac cgccctgtgg
    11281 gcgtttggcc tgacggatga ggtttttgcc gccgcaacag caaaactggt acgcaataat
    11341 cgccgctgga gcgagaactg gatgatcggc attgccttca gttcatggtc atcgtgggta
    11401 tttggtacgg taataggggc attctccggc agcggcttgc tgcaaggtta tcccgccgtt
    11461 gaagcagcat taggttttat gcttccggca ctctttatga gtttcctgct cgcctctttc
    11521 cagcgcaaac aatctctttg cgttaccgca gcgttagttg gtgcccttgc aggcgtaacg
    11581 ctattttcta ttcccgtcgc cattctggca ggcattgtct gtggctgcct cactgcgtta
    11641 atccaggcat tctggcaagg agcgcccgat gagctatgag gttctgctgc ttgggttact
    11701 ggttggcgcg gcgaattatt gcttccgcta tttgccgctg cgcctgcgtg tgggtaatgc
    11761 ccgcccaacc aaacgtggcg cggtaggtat tttgctcgac accattggca tcgcctcgat
    11821 atgcgctctg ctggttgtct ctaccgcacc agaagtgatg cacgatacac gccgtttcgt
    11881 gcccacgctg gtcggcttcg cggtactggg tgccagtttc tataaaacac gcagcattat
    11941 catcccaaca ctgcttagtg cgctggccta tgggctcgcc tggaaagtga tggcgattat
    12001 ataacccaca agaatcattt ttctaaaaca atacatttac tttatttgtc actgtcgtta
    12061 ctatatcggc tgaaattaat gaggtcatac ccaaatggat agttcgttta cgcccattga
    12121 acaaatgcta aaatttcgcg ccagccgcca cgaagatttt ccttatcagg agatccttct
    12181 gactcgtctt tgcatgcaca tgcaaagcaa gctgctggag aaccgcaata aaatgctgaa
    12241 ggctcagggg attaacgaga cgttgtttat ggcgttgatt acgctggagt ctcaggaaaa
    12301 ccacagtatt cagccttctg aattaagttg tgctcttgga tcatcccgta ccaacgcgac
    12361 gcgtattgcc gatgaactgg aaaaacgcgg ttggatcgaa cgtcgtgaaa gcgataacga
    12421 tcgccgctgc ctgcatctgc aattaacgga aaaaggtcac gagtttttgc gcgaggtttt
    12481 accaccgcag cataactgcc tgcatcaact ctggtccgcg ctcagcacaa cagaaaaaga
    12541 tcagctcgag caaatcaccc gcaaattgct ctcccgtctc gaccagatgg aacaagacgg
    12601 tgtggttctc gaagcgatga gctaacgcgt catctcgctc aaaaatccag atttataaaa
    12661 gaaaaaatga ccggccagca tcgcaacatg ctggcctttt tggcaagcag gtcggctcag
    12721 ccgatgagtt aagaagatcg tggagaacaa tatgagcgca aatgcggaga ctcaaacccc
    12781 gcagcaaccg gtaaagaaga gcggcaaacg taagcgtctg ctcctccttc tcaccttgct
    12841 ctttataatt attgccgtag cgatagggat ttattgtttt ttggtactgc gtcatttcga
    12901 agaaaccgat gacgcatacg tggcagggaa tcagattcaa attatgtctc aggtgtctgg
    12961 cagcgtgacg aaagtctggg ccgataacac cgattttgta aaagaaggcg acgtgctggt
    13021 cactctcgac ccgacagatg ctcgccaggc gtttgaaaaa gccaaaactg cactggcttc
    13081 cagcgttcgc caaacccacc agctgatgat taacagcaaa cagttgcagg cgaatattga
    13141 ggtgcagaaa atcgccctcg caaaagcaca aagcgactac aaccgccgtg tgccgctggg
    13201 caatgccaac ctgattggtc gcgaagagct gcaacacgcc cgcgacgccg tcaccagtgc
    13261 ccaggcgcaa ctggacgtcg ctattcaaca atacaatgcc aatcaggcga tgattctggg
    13321 gactaaactg gaagatcagc ctgccgtgca acaggctgcc accgaagtac gtaacgcctg
    13381 gctggcgctg gagcgtaccc gtattgtcag tccgatgacc ggttatgtct cccgccgcgc
    13441 ggtacagcct ggggcgcaaa ttagcccaac gacgccgctg atggcggtcg ttccagccac
    13501 caatatgtgg gtggatgcca actttaaaga gacgcagatt gccaatatgc gtatcggtca
    13561 gccggtcact atcaccacgg atatttacgg cgatgatgtg aaatacaccg gtaaagtggt
    13621 tggtctggat atgggcacag gtagcgcatt ctcactgctt ccggcgcaaa atgcgaccgg
    13681 taactggatc aaagtcgttc agcgtctacc tgtgcgtatc gaactggacc agaaacagct
    13741 ggagcaatac ccgctgcgca ttggtttgtc cacactggtg agcgtcaata ccactaaccg
    13801 tgacggtcag gtactggcaa ataaagtacg ttcaacaccg gtcgcggtaa gtaccgcgcg
    13861 tgaaatcagc ctggcacctg tcaataaact gatcgacgat atcgtaaaag ctaacgctgg
    13921 ctaatccaga ggtgcgtgtg atgcaacagc aaaaaccgct ggaaggcgcg caactggtca
    13981 ttatgacgat tgcgctgtca ctggcgacat tcatgcaggt gctggactcc accattgcta
    14041 acgtggcgat ccccactatc gccgggaatc tgggctcatc gctcagccag ggaacgtggg
    14101 taatcacttc tttcggggtg gcgaatgcca tctcgatccc gcttaccggc tggctggcaa
    14161 agcgcgtcgg ggaagtgaaa ctgttccttt ggtccaccat cgcctttgct attgcgtcgt
    14221 gggcgtgtgg tgtctccagc agcctgaata tgctgatctt cttccgcgtg attcagggga
    14281 ttgtcgccgg gccgttgatc ccgctttcgc aaagtctatt gctgaataac tacccgccag
    14341 ccaaacgctc gatcgcgctg gcgttgtggt cgatgacggt gattgtcgcg ccaatttgcg
    14401 gcccgatcct cggcggttat atcagcgata attaccactg gggctggata ttcttcatca
    14461 acgtgccgat tggcgtggcg gtggtgttga tgacactgca aactctgcgc ggacgtgaaa
    14521 cccgcaccga acggcggcgg attgatgccg tggggctggc actgctggtt attggtatcg
    14581 gcagcctgca gattatgctc gaccgcggta aagagctgga ctggttttca tcacaggaaa
    14641 ttatcatcct taccgtggtg gcggtggtgg ctatctgctt cctgattgtc tgggagctga
    14701 ccgacgataa cccgatagtc gatctgtcgt tgtttaagtc gcgcaacttc accatcggct
    14761 gcttgtgtat cagcctcgcg tatatgctct acttcggcgc tattgttctg ctgccgcagt
    14821 tgttgcagga ggtctacggt tacacggcga cctgggcagg tttggcctct gcgccggtag
    14881 ggattattcc ggtgatcctg tcgccgatta tcggccgctt cgcgcataaa ctggatatgc
    14941 ggcggctggt aaccttcagc tttattatgt atgccgtctg cttctactgg cgtgcctata
    15001 cctttgaacc aggtatggat tttggcgcgt cggcctggcc gcagtttatc caggggtttg
    15061 cggtggcctg cttctttatg ccgctgacca ccattacgct gtctggtttg ccaccggaac
    15121 gactggcggc ggcatcgagc ctctctaact ttacgcgaac gctggcgggg tctatcggca
    15181 cgtcgataac cacgaccatg tggaccaacc gcgagtcgat gcaccatgcg cagttgactg
    15241 agtcggtaaa cccgttcaac ccgaatgccc aggcgatgta cagtcaactg gaagggcttg
    15301 ggatgacgca acagcaggca tcaggctgga ttgcccagca gatcaccaat caggggctga
    15361 ttatttccgc caatgagatc ttctggatgt cagccgggat attcctcgta ctgctggggc
    15421 tggtgtggtt tgctaaaccg ccatttggcg caggtggcgg tggcggcggt gcgcactaag
    15481 tacaactaag ccagttcatt tgaactggct tttttcaatt aattgtgaag atagtttact
    15541 gactagatgt gcagttcctg caacttctct ttcggcagtg ccagttcttc gttgctgttg
    15601 atgcgtacgt cacgttccag aatgctacgc gcaatatcct gcgcttcctg caacgagtgc
    15661 atctggtaag tgccacactg gtagacgttc agttccggga tctgattctg atcctgcact
    15721 ttcagcacgt cttccattgc cgctttccag gcatcagcaa cacgctgctc atctggcgta
    15781 ccaatcagac tcatataaaa accggtgcgg cagcccattg gcgagatatc gataatctct
    15841 acaccattac cgttaagatg gttacgcata aaaccagcaa acaggtgctc cagggtatgg
    15901 atccctcttt ctggcatcac ttctttgttc ggcacgcaga agcgcagatc gaacacggtg
    15961 attgcgtcgc catgcggggt gttcattgtt ttcgccaccc gaactgcagg cgcttccatc
    16021 cgggtatgat cgactgtgaa gctatctaac aacggcattt agccacctcc ggtaattttt
    16081 ttaaaaattt tctgaactct ttcttcccag gcgagtctga gtatatgaaa gacgcgcatt
    16141 tgttatcatc atccctgaat tcagagatga aattttggcc actcacgagt ggcctttttc
    16201 ttttctgtca ggtgtgtttt tccagccaca ccgcaaacgg ttcggtatca gcggtttcca
    16261 tttcctgctg acggcgttca gatgcctcgc gctcggctac aaaatcctct tcgcgcagaa
    16321 tttccagcgg ctcttcacgc agcagattac ggtaggcttc tgcaaacgct ttgcctgttc
    16381 cgccaatacc agtatcaatc atagacctta agatacgggc agagaaagtc agatcgggat
    16441 tatcgaagca ggcaaccagt tcatcacaca ctttctgata cgcgtcgccg ccgttaatgc
    16501 tatccagcgt ttgcgcgacg cgtttcagat cgcggaacag atctttaccc acctgcagta
    16561 acgggaactg cgcggtttcg cagccgatac ccagcgtcag gcccggtttg cgaccttcaa
    16621 ggatcacccg gttccagtta acgcgcgtac aggcaagttc gctactgctc atttccggtg
    16681 catcagccag cgcacaccag accataaaca ggtcgaggaa tcgcacctgc tgttcatcta
    16741 caccaatcgg cgagaacggg ttgatgtcca gcgaacgcac ttcaatatat tcaatgccgc
    16801 cacgtaacag cgcatcagaa ggtgactcgc cgctgcgggt aacgcgtttt ggacgaatcg
    16861 gcgcatacag ttcgttttca atctgcagca cgttgctatt gatttgcagc ctcttaccgt
    16921 ctttctcaat accaatcttc gcgtactctt ccgatggcgt tttgattgcc tgtttaaggc
    16981 ccgctacgta ctcgtaaaga tcgttgaagg taataccaag attgctttgc gatttattgg
    17041 tatagccgag atcgctcaaa cgaagagagg tcgcatacgg caggtaatac ataccgcact
    17101 cggttttctc aaacggcagc gacgttggtt ttccttgcag gaaagaagaa caaatcgccg
    17161 gagatgcacc aaacagataa ggaatgaccc aaccgaaacg atagtaattg cggataacgc
    17221 ggaaatagcc cgcagaaatt ttctctttgg catcagcgcc cgagatatca ccgcacttcg
    17281 cttgccagaa tgccattggc aaagagaaat tgtagtgcac gccggaaatg gtttgcatca
    17341 gcgcgccgta gcgatttttc agcccttcac gatacagcgt tttaaagcgt ccggtgttag
    17401 aagtgccgta ctgtgccagt tcgatgtcct gaccttctgc gatgtagcat ggcatactta
    17461 acggccacat ccgctcatcg cccatattgc gcgccgtata acgatgcaga tcgcgcataa
    17521 aggtcagcat atgttcaata tcaccatcca ctggtgtaat gaattccagc aatgcttccg
    17581 caaaatcggt agtaatccat ttgtgcgtca gtgcggaacc taatgcttca ggatgacctg
    17641 ttgttgccag tgtgccatca gcattaacac gcaaagtttc gcgctccagc ccacgctgta
    17701 tcccctttaa cgcctgagga tgtttttcca gccaggccag cgcctgtgat acgtccggga
    17761 tcaaattgac ctcccgcctg tcaaaatcgt tttaattagc ataactgtaa tggtgaccat
    17821 atgtgcaggc ctacaattag tgccaccaca tcatgccctg aacggtcgct gctgcaactg
    17881 caacatagcg taacgcttta ccaaggcaca aaaaaaagat taccggtccc cacgagatgc
    17941 gcatccatcc cgctaacaga cacagtaaat cgccaaccac gggcatccag cttaataata
    18001 gcgtgactgc accatagcgt ttcagccagc cgatagcttt ctcttgccag cgcgatgttt
    18061 tacgcaatgg aaagaaacgc ccaaggataa cgttagttaa ccctccaagg ctattaccca
    18121 ttgttgctgt taaaactaaa acccagggat gactgatccc ggaaagcaac attgccacca
    18181 gcacgacttc ggagttgccg ggtaatagtg tagcgctgag aaaactactg gcaaacaacg
    18241 agaaaagcga taacgcttca ctcacagcaa gcgaacgtcc acagcgtcca tacctgcggc
    18301 acgggccgcc tgaataccga aatcggcatc ttcaaagacc acacactgcg tcggttgcac
    18361 gcccatacgc tgcgcgcaca acaaaaatgt gtctggcgcg ggtttatggt gtttgacgtg
    18421 atcggcagcg acgacggcgt caaaataatg gcgtaatccc aggtgcgcca gcaatgcctc
    18481 agcgatggcg ctttcactcc ccgttcctac agccattggg cgacgaccat gccaactttt
    18541 caccacatca acaagaggaa gcggttcgat gctatccagc agcatacttc ttactgcttc
    18601 tgttttttca cgcgctaacg catgcgggtc gagatcggcc tgattcagct caataattgc
    18661 ctgagcaata cgccaggtgg gcgatccatt aagcgcaatc atcgcctgaa tatcgtactg
    18721 aagaccgtag tgccctaata cttcgcgcca cgctttacgg tgcgtaggct ccgtatccag
    18781 gattgtgcca tccatatcaa aaattaaacc tgcataacgc tcgtacatgg tcttctcgca
    18841 agtcgaaaaa tatgacgcta ctttagcgta attgcttgat tttgtcgctg atggcgaagg
    18901 agaagaaaat tcaggaaatg gataaaggag ttatttagaa atgagatatt tttgaaggaa
    18961 attttttgga gaagatggtg catccgggag gattactcgc cttgcggctc gcccttcggg
    19021 ccgttgctgg cgcaacgtta tccttcctgg tgcctgcggt taataccgct atctcttcga
    19081 taccttcatt gctgaaggtt acggagaata tggtgcatcc gggaggattc gaacctccga
    19141 ccgctcggtt cgtagccgag tactctatcc agctgagcta cggatgcatc gggaaaactt
    19201 attctactgc tgatattgat accgctacta aagccatatc aattaagaga tggtgcatcc
    19261 gggaggatta ctcgccttgc ggctcgccct tcgggccgtt gctaaagcaa cgttatcatc
    19321 cctggtgcct gcggttaatt ccgctacctc ttcgatacct tcattgctga agatttcgga
    19381 gaatatggtg catccgggag gattcgaacc tccgaccgct cggttcgtag ccgagtactc
    19441 tatccagctg agctacggat gcatcgggaa aatttatttt actgctgata ttgataccgc
    19501 tactaaagcc atatcaagta agagatggtg catccgggag gattcgaacc tccgaccgct
    19561 cggttcgtag ccgagtactc tatccagctg agctacggat gcaaatggcg gtgaggcggg
    19621 gattcgaacc ccggatgcag cttttgaccg catactccct tagcagggga gcgccttcag
    19681 cctctcggcc acctcaccac acgcctctta cgagtgcttc gaagaacttg tttatcgctc
    19741 atcgtcgctg cgtggcgcac atattacttt ctgagactta taagtcaaac aatttttccc
    19801 acacttttat cgtttgcaca cttcacgctc aattagtctg taaaaacgtc aaaaagagtg
    19861 ttttatctac agaaaaacgg aggtctggca gatagcagta atgcaaaaaa atggagactt
    19921 aagttgaatg aacgggagta aagcgaaaag actatagagt gaaggagaaa ttttgagggt
    19981 gcgtctcacc gataaagatg agacgcggaa agattagtaa ctggactgct gggatttttc
    20041 agcctggata cgctggtaga tctcttcacg gtgaacagaa acttccttcg gggcatttac
    20101 gccaatacgt acctggttgc cctttacccc taaaactgtc acggtgacct catccccaat
    20161 catgagggtc tcaccaactc gacgagtcag aatcagcatt ctttgctcct tgaaagatta
    20221 aaagagtcgg gtctctctgt atcccggcat tatccatcat ataacgccaa aaagtaagca
    20281 atgacaaaca cattacatgt aagcagtcat ggcattacat tctgttaaac ctaagtttag
    20341 ccgatataca caacttcaac ctgactttat cgttgtcgat agcgttaatg cgaatgccgt
    20401 gaagcgagtc cacggcattg cctgacgctt atattattgc aatttcgcgc tgacccagcc
    20461 tttcacactg gctaacgctg caggtaaggc cgcagcatcc gtaccaccgg cttgcgccat
    20521 gtcaggacgt ccaccaccct tgccgcccac ctgctgagcg accataccaa tcagttcccc
    20581 tgctttcaca cgatctgtga cgtccttaga tacgcctgca atcagagaaa ccttaccttc
    20641 ggctaccgtt gccagcacga taattgtcga ccccagctga ttttttaaat cgtcaaccat
    20701 ggtacgcaac attttcggct caacaccgct aagctcgcta accaacagct taacaccatt
    20761 aacatcaatt gccttactgg aaagatttgc gctctcctgt gcggcagctt gttctttaag
    20821 ctgttgtagt tctttttcta actgacgcgt acgttccagt actgagcgca ctttatccgc
    20881 cagattattg ctatcgcctt tcagcagatg cgcgacttcg cttaagcgat cgctgtctgc
    20941 atgaacggtg gtgatagcgc cttctccggt taccgcttcg atacgacgaa cgcctgcagc
    21001 agtacccgat tcagagatga tgcggaacag accaatatca ccagtgcggc tggcgtgagt
    21061 accgccacac agctcggtgg agaaatcgcc catgctcagc acgcgtacac gctcatcata
    21121 cttctcgccg aacagcgcca tcgcaccttt cgctttcgcc gcttcgagat ccatgatgtt
    21181 ggtttcgatc ggcaaattgc gacgaatctg tgcgttcacc aggtcttcga ccgcacgaat
    21241 ctcttccggt ttcatcgctt cgttgtgtga gaagtcgaag cgcagcacct tgtcgttaac
    21301 cagtgaacct ttctgcgata catgagtccc cagaacctgg cgcagcgcag cgtgcatcag
    21361 gtgcgttgcg gagtgattca gacgaatacg ggcgcgacga gcctcatcaa catcagcctg
    21421 cacagcgtcg cccactttca gagaacccgc agcaagttta ccgatgtgac caatcgcctg
    21481 gccgtatttc tgcgtatctt ccaccgcgaa ggagaagtta gcgcctttca gttcgccttt
    21541 atcaccaacc tgaccgccgg attccgcata gaatggcgtt tgatccagca cgaccacagc
    21601 ttcctggcct gcattgatgg catcaaccgc tttaccatca acaaacagcg cggtcacttt
    21661 gccgttcagt tccagatggt catagccttt aaattcagat gcactatcaa cacggatcat
    21721 tgcgttgtaa tcggcaccaa agccgctggc ttcgcgcgcg cgacgacgct gctcttccat
    21781 tgctgcttca aaaccagctt cgtcaacttt gatgttgcgc tcacgacaaa catcagccgt
    21841 caggtcaacc gggaagccat aggtgtcgta cagacggaaa gccgtttcac catccagcgt
    21901 atcaccagaa agttttgcca gctcttcatc cagcaacgcc agaccgcgct ccagagtacg
    21961 ggcaaactgc tcttcttcag tcttcagcac ctgctcaacc tgcgcctgct ggcgtttcag
    22021 gtcttcaccc gcagagccca taacgtcgat cagcggacca accagtttgt agaagaaggt
    22081 ttctttcgcg ccgagcatat tgccgtgacg cactgcgcga cgaatgatac gacgcagtac
    22141 ataaccacgg ttttcattgg acggcattac gccatccgcg atcaggaacg cacaagaacg
    22201 aatgtggtca gcgattacgc gcagcgattt attgctcaga tcggtcgcgc cagtgacttt
    22261 cgctaccgcc tggatcaacg tgcggaacag gtcgatgtca tagttagagt taacgtgttg
    22321 cagcaccgca gcaatacgct ccagacccat accggtatct acagacggct tcggcagcgg
    22381 ttccatcgtg ccatctgcct ggcggttgaa ctgcatgaag acgatgttcc agatctcaat
    22441 gtagcggtcg ccgtcttctt ccggacttcc cggagggcca ccccaaatgt ggtcgccgtg
    22501 atcgtagaag atttcggtgc acgggccgca cggaccagtg tcacccatct gccagaagtt
    22561 gtcagatgcg tatggcgcac ctttgttatc gccgatgcga ataatacgtt cgcgcgggat
    22621 ccctacttct ttttcccaga tttcgtaggc ttcgtcgtcg ctttcataga cggtaaccca
    22681 cagacgctct ttcggcaggg caaaccattt ttcgctggtc agcagttccc atgcaaactg
    22741 aatggcatcg tgtttgaaat agtcgccgaa gctgaagttg cccagcattt cgaagaaggt
    22801 atggtgacgc gcggtgtaac cgacgttttc caggtcgttg tgtttaccac ccgcacgcac
    22861 gcagcgttgg gaagtggtag cgcgggaata attacgcttg tcgagcccaa ggaacacatc
    22921 cttgaactgg ttcatcccgg cgttggtaaa caacaaagtt gggtcgttat ggggtaccag
    22981 ggagctgctg gcaactacct gatgtccctt actatggaaa aagtcgagaa acgcctgacg
    23041 gatctcagcg gtgctcttgc tcataattat cctgaaatca agctaacgaa atatcgccac
    23101 cagctccagc gtgtcttaac cgccgggctg gtaactgaaa agtgggaata agataagttt
    23161 tcttgagtgg gaagtaaaat accgtatgcg ttcagtcggc aaaatttcgc caaatatcct
    23221 ggatatcttc catcagatag ccacgataga gcagaaaacg ctggatctta actttttctg
    23281 aaaagacagt tggcaaaggt tcaccatatt ttcgcgtcgc ctgatcgcgc accagtgcgc
    23341 accagtcgat gtcacattca cgcatcgctt tttctgtcgc ttcgcgggaa atacctttct
    23401 gattcagttc ctgacgaata cgtgcaggtc cataaccttt gcggctacgg ctggcgataa
    23461 agcgcgcaac aaatcggctg tcatcgagat agccatgttc atggcaccag gcaataacgc
    23521 gctcgtaatc ttctgccgta gcatcaatct cttctgggcc atttttgccc ataatcggtg
    23581 ccgcgagttt acgtcgcagt tcttgctcac tgtgatcgcg caccgccaga atgcgtaccg
    23641 cacgatccaa caggcgagca tatgccgggc gacgggatgt tgattctgtc atggcatatc
    23701 cttacaactt aaaaaagcaa aagggccgca gatgcgaccc aggttttgct gataaacaga
    23761 tttaatagtc aatgagtgtg cagccaattc gttagaggat cgaggtgaga gtaatttttc
    23821 acgccagtgt tggcctgttc ccctcaccct aaccctctcc ccaaaggggc gaggggaccg
    23881 tccgtgcaca cattatctat tatcatcaat aacgaacgat taaaaatctt cgttagtttc
    23941 tgctacgcct tcgctgtcat ctacagagaa atccggcgtt gagttcgggt tgctcagcag
    24001 caactcacgt actttcttct cgatctcttt cgcggtttcc gggttatctt tcagccaggc
    24061 agttgcattc gctttaccct gaccgatctt ctcacctttg tagctgtacc acgcgcctgc
    24121 tttctcgatc agcttctctt ttacgcccag gtcaaccagt tcgccgtaga agttgatacc
    24181 ttcgccgtag aggatctgaa attcagcctg tttaaacggt gcagcgattt tgttcttcac
    24241 cactttcacg cgggtttcgc tacccaccac gttttcgccc tctttcaccg cgccgatacg
    24301 acggatgtcg agacgaacag aggcgtagaa tttcagcgcg ttaccaccgg tagtggtttc
    24361 cgggttaccg aacatcacac caattttcat acggatctgg ttgatgaaga tcagcagcgt
    24421 gttggactgc ttcaggttac ccgccagctt acgcatcgcc tggctcatca tacgtgccgc
    24481 aaggcccatg tgagagtcgc cgatttcgcc ttcgatttcc gctttcggcg tcagtgccgc
    24541 cacggagtca acgacgataa cgtctactgc gccagaacgc gccagggcgt cacagatttc
    24601 cagtgcctgc tcgccggtgt ccggctggga gcacagcagg ttatcgatat cgacgcccag
    24661 tttacgtgcg tagattgggt ccagcgcgtg ttcagcatcg ataaacgcac aggttttacc
    24721 ttcacgctgc gctgcggcga tcacctgcag cgtcagcgtg gttttaccgg aagattccgg
    24781 tccgtagatt tcgacgatac ggcccatcgg cagaccacct gccccaagcg cgatatccag
    24841 tgaaagcgaa ccggtagaga tggtttccac atccatggaa cggtcttcac ccaggcgcat
    24901 gatggagcct ttaccaaatt gtttctcaat ctggcccagt gctgccgcca acgctttctg
    24961 tttgttttcg tcgatagcca tttttactcc tgtcatgccg ggtaataccg gatagtcaat
    25021 atgttctgtt gaagcaatta tactgtatac tcatacagta tcaagtgttt tgtagaaatt
    25081 gttgccacaa ggtctgcaat gcatacgcag tagcctgacg acgcaccgca tcacggtcgc
    25141 cgctgaagca ttcccgccgg gtaatgcctt caccgcgggc agtggcaaaa gcaaaccaga
    25201 cggtgccgac aggcttctct tcactgccgc catccggccc ggcgatacca ctaatagaca
    25261 cggcataatc agcacgagcc gctttcagtg cacctatcgc catttccacc acgacgggtt
    25321 cactcaccgc gccatgctgc gccagcgtct cttcgcgtac gccgatcatc tgcgctttgg
    25381 cttcgttact gtaggtgaca aatccgcgtt caaaccaggc ggagctaccg gcaatatcgg
    25441 taatcacttt cgctacccaa ccaccggtac aagactcggc agttgttacg gttgcgccac
    25501 gggctttcag cgcctgccca acctgttcgc ttaactgcat cagttcactg tcagtcatca
    25561 caacctcagt cagtcaaaaa tttatgccgg acaagatagc actttcgtca ggaagatggt
    25621 gttggcgttg caattttacg aggaggattc agaaaaaagc tgattagcca gagggaagct
    25681 cacgcccccc tcttgcaaat agttactgca cgcgcgccag cgccacggct tgtcctaact
    25741 gccagaccgc cattgcgtaa tgggtgctgt ggttataacg ggtaatggtg tagaagttcg
    25801 gcagaccgta ccagtactgg taaccggtac cgacatccag acgcagcagg ctggcttgtt
    25861 gatggttgcc cagcggctgc tgtggcgtta aacctgccgt ggcaagctgc gaaatgctgt
    25921 acctggtttt gaagccattt ggcaagcctg gagcctgacc gtttgccatt accgcgacct
    25981 gatcgccttt cacccagccg tgcgctttga aatagttcgc cacactaccg atcgcatcaa
    26041 ccggatccca caggttgata tgcccgtcgc cgctgaaatc taccgcatat tgtttgtaag
    26101 acgacggcat aaactgtccg tagcccatcg ccccggcaaa ggaacctttc agattgagcg
    26161 gatcgtcctg ctcgtcgcgc gccatcaaca ggaaggtttc cagctcgcca gagaaatact
    26221 ccgcgcggcg tgggtagtta aatgacagcg ttgccagcgc atcgaggatg cgagttttcc
    26281 ccatcacgcg cccccagcgg gtttcaacgc cgataatccc gacgataatt tccggcggta
    26341 cgccatacac ctgccacgcg cgattcaacg catcttcata ctgattccag aaaaccacac
    26401 cgttctgcac gttgtccggc gtaataaatt ttttgcgata acgcagccat gcgccgttcg
    26461 gacctgatgg cggtttcacc gatgtggttg gtgcctggtt atccatcagc cgcagtaccg
    26521 aatccagacg cttcgcctgg gagagaattt cctgcaactg ctgacgatcg aaaccgtgtt
    26581 tgttcaccat tctgtcgatg aactgctggg cattcgggtt attagcgaaa tcgccgccca
    26641 tctgcatcac attgtgctga ggctcaagca ggaagccgcc agacggcgtt ccggtggtcg
    26701 tttcagtctc agtaggtttt ggcttgctgc tacaggcggc aagcaacaca aaaaggggaa
    26761 gcaatgttac ataacgacgc ttgaacatga ggggtccatt taacagattc aaccaggggc
    26821 aagtatggta aagcatcacg ccccgcacaa ggaagcggta gtcactgccc gatacggact
    26881 ttacataact caactcattc ccctcgctat ccttttattc aaactttcaa attaaaatat
    26941 ttatctttca ttttgcgatc aaaataacac ttttaaatct ttcaatctga ttagattagg
    27001 ttgccgtttg gtaataaaac aataaatcct gaaggagaga acaatgatag aaaccattac
    27061 tcatggtgca gagtggttta tcgggctgtt ccaaaagggc ggagaggtgt ttaccgggat
    27121 ggtgaccggc attcttccgc tgttgattag cctgctggtt atcatgaacg cactgattaa
    27181 ttttatcggt cagcatcgta ttgaacgttt tgctcaacgt tgcgccggta accctgtttc
    27241 ccgttacctg ctgctaccgt gcattggcac gtttgtcttt tgcaatccga tgaccttaag
    27301 cctgggtcgc tttatgccgg aaaagtacaa acccagctac tacgcggcag cctcttatag
    27361 ctgccactca atgaatggcc tctttcccca tatcaaccct ggcgaactgt ttgtttatct
    27421 tggcattgcc agcggtctga caacgctgaa cctgccactt ggcccactgg cggtgagcta
    27481 tctgctggtt ggtctggtca ccaacttctt tcgcggctgg gtgaccgatc tgaccaccgc
    27541 catttttgag aaaaagatgg gcattcaact tgaacaaaaa gttcacctgg caggagcaac
    27601 atcatgacgc gtattcggat cgaaaaagga acgggtggct ggggcggccc gcttgagctg
    27661 gaagccacgc cgggcaaaaa aatcgtctat atcaccgccg gtacccggcc tgcgattgtt
    27721 gacaaactgg cacaacttac tggctggcag gctattgacg gatttaaaga aggtgaaccc
    27781 gcggaggcgg aaattggtgt cgcggtaatc gactgtggcg gcacattacg ctgcggcatc
    27841 tatccgaaac gacgtattcc caccattaat atccactcga cgggcaagtc cagtccgctg
    27901 gcgcagtaca ttgtggaaga tatttatgtc tctggcgtaa aagaagaaaa catcactgta
    27961 gtgggtgatg cgacaccaca accctcttcc gtgggccgtg actatgacac cagcaagaaa
    28021 atcaccgaac aaagcgatgg tttactggcg aaggtgggaa tgggtatggg ttctgccgtt
    28081 gccgtgttgt ttcaatctgg tcgtgacacc atcgacactg tattaaaaac cattctgccg
    28141 tttatggcgt tcgtctcggc gctcattggc atcattatgg cttctggcct tggtgactgg
    28201 attgcccacg gtcttgctcc gctggcgagc catccactgg gtctggtcat gctggcgctc
    28261 atctgctcct tcccgctgct ttcacctttc ctcggcccag gcgcagttat cgcacaggtt
    28321 atcggcgtat tgattggcgt gcagattggt ctcggcaata ttccgccgca tctggcttta
    28381 ccggcactgt ttgccatcaa cgcgcaggcg gcctgcgact tcatcccggt cggtttgtcg
    28441 ctggcggaag cccgtcagga cacggttcgc gtcggtgtcc cttctgtact ggtgagccac
    28501 tttttaaccg gcgcaccgac tgtactgatc gcctggtttg tctccggttt tatctatcaa
    28561 tagaggctga aacatgaccg ttatttatca gaccaccatc acccgtatcg gcgcgagtgc
    28621 cactgacgcc ct
//
LOCUS       DAZPLI010000038        28260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZPLI010000038 DAZPLI010000000
VERSION     DAZPLI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.038"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHUU3_001613"
     CDS             complement(<1..215)
                     /locus_tag="ACHUU3_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR2081709.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHUU3_001614"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHUU3_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR2081710.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHUU3_001615"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHUU3_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR2081711.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHUU3_001616"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHUU3_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR2081712.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHUU3_001617"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHUU3_001617"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR2081713.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHUU3_001618"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHUU3_001618"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR2081714.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHUU3_001619"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHUU3_001619"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR2081715.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="ACHUU3_001620"
     CDS             complement(4604..5326)
                     /locus_tag="ACHUU3_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR2081716.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHUU3_001621"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHUU3_001621"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR2081717.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="ACHUU3_001622"
     CDS             6526..6744
                     /locus_tag="ACHUU3_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR2081718.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHUU3_001623"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHUU3_001623"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR2081719.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="ACHUU3_001624"
     CDS             complement(7478..7678)
                     /locus_tag="ACHUU3_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR2081720.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHUU3_001625"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHUU3_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFR2081721.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHUU3_001626"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHUU3_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR2081722.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="ACHUU3_001627"
     CDS             10175..12088
                     /locus_tag="ACHUU3_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR2081723.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="ACHUU3_001628"
     CDS             12088..13110
                     /locus_tag="ACHUU3_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR2081724.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="ACHUU3_001629"
     CDS             13104..13322
                     /locus_tag="ACHUU3_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR2081725.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="ACHUU3_001630"
     CDS             13376..14245
                     /locus_tag="ACHUU3_001630"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR2081726.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="ACHUU3_001631"
     CDS             complement(14300..14704)
                     /locus_tag="ACHUU3_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR2081727.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="ACHUU3_001632"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="ACHUU3_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR2081728.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGSR"
     gene            15689..17779
                     /locus_tag="ACHUU3_001633"
     CDS             15689..17779
                     /locus_tag="ACHUU3_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR2081729.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHUU3_001634"
                     /gene_synonym="dapC"
     CDS             complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHUU3_001634"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR2081730.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHUU3_001635"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHUU3_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR2081731.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="ACHUU3_001636"
     CDS             complement(19747..20349)
                     /locus_tag="ACHUU3_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR2081732.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="ACHUU3_001637"
     CDS             complement(20339..20506)
                     /locus_tag="ACHUU3_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR2081733.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHUU3_001638"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHUU3_001638"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR2081734.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHUU3_001639"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHUU3_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFR2081735.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="ACHUU3_001640"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="ACHUU3_001640"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR2081736.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="ACHUU3_001641"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="ACHUU3_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR2081737.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="ACHUU3_001642"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="ACHUU3_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR2081738.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cysG"
                     /locus_tag="ACHUU3_001643"
     CDS             26714..28087
                     /gene="cysG"
                     /locus_tag="ACHUU3_001643"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR2081739.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6837 a         7592 c         7131 g         6700 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccgaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgccaagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gccgctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggct ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt cgccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agagaattct ggccttctta atgactcttt ttatatattc
    28141 agcaaataaa acataacccc ttaataaata ttatctgcta atgggttata ttcaccgtta
    28201 cagcgactta caaaattaag tcattcgctc actgacgcca attaaatatt cctgtatcgt
//
LOCUS       DAZPLI010000039        28050 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZPLI010000039 DAZPLI010000000
VERSION     DAZPLI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28050
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.039"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..248
                     /gene="tnpA"
                     /locus_tag="ACHUU3_001644"
     CDS             <1..248
                     /gene="tnpA"
                     /locus_tag="ACHUU3_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFR2081740.1"
                     /translation="MDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRPDIAV
                     RYYYKGVLWSPGYFANSCGGAPISVIRQYIEQQQTPG"
     gene            308..382
                     /locus_tag="ACHUU3_001645"
                     /pseudo
     CDS             308..382
                     /locus_tag="ACHUU3_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(379..1702)
                     /locus_tag="ACHUU3_001646"
                     /pseudo
     CDS             complement(379..1702)
                     /locus_tag="ACHUU3_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1702..3318)
                     /locus_tag="ACHUU3_001647"
     CDS             complement(1702..3318)
                     /locus_tag="ACHUU3_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR2081741.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3614..4330
                     /locus_tag="ACHUU3_001648"
     CDS             3614..4330
                     /locus_tag="ACHUU3_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR2081742.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4327..5988)
                     /locus_tag="ACHUU3_001649"
     CDS             complement(4327..5988)
                     /locus_tag="ACHUU3_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR2081743.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6184..6612)
                     /gene="ibpB"
                     /locus_tag="ACHUU3_001650"
     CDS             complement(6184..6612)
                     /gene="ibpB"
                     /locus_tag="ACHUU3_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR2081744.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6724..7137)
                     /gene="ibpA"
                     /locus_tag="ACHUU3_001651"
     CDS             complement(6724..7137)
                     /gene="ibpA"
                     /locus_tag="ACHUU3_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR2081745.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7131..7203)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7443..7775
                     /locus_tag="ACHUU3_001652"
     CDS             7443..7775
                     /locus_tag="ACHUU3_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR2081746.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7777..8991)
                     /locus_tag="ACHUU3_001653"
     CDS             complement(7777..8991)
                     /locus_tag="ACHUU3_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR2081747.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9092..10156
                     /gene="cbrA"
                     /locus_tag="ACHUU3_001654"
     CDS             9092..10156
                     /gene="cbrA"
                     /locus_tag="ACHUU3_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR2081748.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10153..11490)
                     /locus_tag="ACHUU3_001655"
     CDS             complement(10153..11490)
                     /locus_tag="ACHUU3_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2081749.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11565..12713)
                     /gene="dgoD"
                     /locus_tag="ACHUU3_001656"
                     /pseudo
     CDS             complement(11565..12713)
                     /gene="dgoD"
                     /locus_tag="ACHUU3_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12710..13327)
                     /gene="dgoA"
                     /locus_tag="ACHUU3_001657"
     CDS             complement(12710..13327)
                     /gene="dgoA"
                     /locus_tag="ACHUU3_001657"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR2081750.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13311..14189)
                     /gene="dgoK"
                     /locus_tag="ACHUU3_001658"
     CDS             complement(13311..14189)
                     /gene="dgoK"
                     /locus_tag="ACHUU3_001658"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR2081751.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14186..14875)
                     /gene="dgoR"
                     /locus_tag="ACHUU3_001659"
     CDS             complement(14186..14875)
                     /gene="dgoR"
                     /locus_tag="ACHUU3_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR2081752.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15153..15809
                     /locus_tag="ACHUU3_001660"
     CDS             15153..15809
                     /locus_tag="ACHUU3_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2081753.1"
                     /translation="MKLNLKGFFKTAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLKDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYNWSTEEEKARVTQYRFACGVQHCTRTVENLEGTIHEKNKN
                     MDYSNVMAFYHPFKVRFYEYHSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(15855..16667)
                     /gene="yidA"
                     /locus_tag="ACHUU3_001661"
     CDS             complement(15855..16667)
                     /gene="yidA"
                     /locus_tag="ACHUU3_001661"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR2081754.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16782..17180)
                     /locus_tag="ACHUU3_001662"
     CDS             complement(16782..17180)
                     /locus_tag="ACHUU3_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR2081755.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17420..19834)
                     /gene="gyrB"
                     /locus_tag="ACHUU3_001663"
     CDS             complement(17420..19834)
                     /gene="gyrB"
                     /locus_tag="ACHUU3_001663"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR2081756.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19863..20936)
                     /gene="recF"
                     /locus_tag="ACHUU3_001664"
     CDS             complement(19863..20936)
                     /gene="recF"
                     /locus_tag="ACHUU3_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR2081757.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20936..22036)
                     /gene="dnaN"
                     /locus_tag="ACHUU3_001665"
     CDS             complement(20936..22036)
                     /gene="dnaN"
                     /locus_tag="ACHUU3_001665"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR2081758.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(22041..23444)
                     /gene="dnaA"
                     /locus_tag="ACHUU3_001666"
     CDS             complement(22041..23444)
                     /gene="dnaA"
                     /locus_tag="ACHUU3_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR2081759.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23741..23821
                     /locus_tag="ACHUU3_001667"
     CDS             23741..23821
                     /locus_tag="ACHUU3_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2081760.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            24051..24191
                     /gene="rpmH"
                     /locus_tag="ACHUU3_001668"
     CDS             24051..24191
                     /gene="rpmH"
                     /locus_tag="ACHUU3_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR2081761.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24208..24567
                     /gene="rnpA"
                     /locus_tag="ACHUU3_001669"
     CDS             24208..24567
                     /gene="rnpA"
                     /locus_tag="ACHUU3_001669"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR2081762.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24531..24788
                     /gene="yidD"
                     /locus_tag="ACHUU3_001670"
     CDS             24531..24788
                     /gene="yidD"
                     /locus_tag="ACHUU3_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR2081763.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24791..26437
                     /gene="yidC"
                     /locus_tag="ACHUU3_001671"
     CDS             24791..26437
                     /gene="yidC"
                     /locus_tag="ACHUU3_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR2081764.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26543..27907
                     /gene="mnmE"
                     /locus_tag="ACHUU3_001672"
     CDS             26543..27907
                     /gene="mnmE"
                     /locus_tag="ACHUU3_001672"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR2081765.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6760 a         7571 c         7158 g         6561 t
ORIGIN      
        1 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctatcctccc aaactggcga
       61 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gttactgcga cgagatcgac
      121 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtcctggc tattttgcca
      181 atagctgcgg aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
      241 ctggttaggt ggaaaaccgc gccttatatc cccgccctga gggacggggt tttacggcgc
      301 accggataaa tttggttatg cgccgtcagc cgtgctaaag aatacggacc agcacccaac
      361 ggatgccagt ccacacaatt aacgtaactc tggccagtac gctttgttgg cctccagcaa
      421 ctcatccaga atagctttgg cgaccgaggc gctcggtacc gttttcgata acgtaatcgc
      481 ctgccacagg tgctgatatg aacgctgttc ccaggcgtcc acgaccagtt tttccaccgc
      541 cacttgctga ctcattaacc ctttctgaaa ctgcgggata tcgccgacca ccagtggttc
      601 tggtccgttg tgcccgacca gacacgggat ctcgaccatc gcttcatcat caaagttatg
      661 gattgccccg ttgttaggca caatcagcaa catccgctcc tgagtgttga aggcaattgc
      721 cgccgccaga tcgacgatgt atgacgcatg ttcgtcaatc tccagcttgg ccgccgctga
      781 atttcctgcc gctgtaatgg cgcggcaagc atcgaaaacc tgtttctcgc gatgttccat
      841 tcacctcatt cgcgcgggta tgttcagggt tggaatgctg aacaacataa tccgggaaga
      901 gataatattt cagataggtg ttcggcaatg tatccgggtc cagtgcctgg acatcccgcg
      961 cttttgcata ggtgtcgttc cagctcgctt cagtatgtgt atcttgctgc ggtggaacat
     1021 aaccgtgctt cgagacatat tgccgcagct ggggcatcag gtcgttgccc tgcaaatctt
     1081 caattgatgt ccaccagcca aagtggttca ggccgtagta gcgcacgcgc atctgtttgc
     1141 gatcttgcag cccaacaatt tgcgccatcc ggctttcaat accgattggc atgtcacaga
     1201 tgttgaggat tttcgcattc gggcgcagac gacgcgtgtc ttctgcgaca atggctgccg
     1261 ggttggagta gttgagcatc caggcatttg gtgaatattt ttccatataa tccaccagtt
     1321 ccaggacgcc gccaatggaa cgcatgccgt acgctattcc gccgggtccg caagtttcct
     1381 gaccaacaac accgtggcgc agtgggattt tttcatccag ttcgcgcatc gggtacttgc
     1441 cgacgcggat gtgcgccatg acaaaatcaa cgtcgctgaa cgccacttca ggatcggtgg
     1501 tgtaactaaa cgcaatgtcc ggcgctttct ctttaaggat gactttacag gcttcggcaa
     1561 tcacttcctg ccgcgcgcca tcgttatcat aaaatttcag tgcacgaagc gggaaacgct
     1621 cctgattcgc caggagcatc aacacgatgc ctggcgtaaa ggtgcttcca ccgcctgcga
     1681 caaccactga gaatttggtc attatactgc ctccgtaatg gcaacatttt ctgctgattg
     1741 atgagaatta attaagctat cgagctgttc acgcagctgg gatacatgca gaccaatgat
     1801 cacctgaatg gcatcgccac tacggaagac gccgtgcgct cccagctttt taaagacttc
     1861 gtcatccagc gtttgtgaca tgtcatgcag tgcaatgcgt agacgcgtcg cacaattgtt
     1921 aatgctggag atattgccga ccccgcccag ggcttgcagg ataccggcag cttgatccag
     1981 ctcttttttt ggctctgccg cggtggtttg gcctcgcgag gctttgtatt cggcttttga
     2041 gtagagtttc acttccgcat cttcacgtcc cggcgtgcac atgttgaact gcagaatcag
     2101 ggtgcggaaa accacgaagt acagcagggt aaagcacaac ccaatggcga tttgggtcag
     2161 catcatatcc gcgtggttgc tgaacatcgg gatccagttt tgcggtaaaa cctggtcaat
     2221 cagacctccg cccatgttgc ccaccacacc aaagagatac atcacggtcg acattgaggc
     2281 cgcaagcaca gcgtgtaccg caaacagcaa cggtgaaatg aacaggaagg taaattccag
     2341 cggttcggta attcccacca gcatggcggt taaggttgcg ggaatcagca agcccgccac
     2401 ttttacccga ttttccggtg cggcagtgaa gtacatcgct aaagaaatgc ccacggcacc
     2461 aaagattttt gagttaccgt gcagggcaaa accgccttcc gggaacaacg atttcagcgg
     2521 ctcggcgctc aaactgaact cttgcagatg ctgcgcccag tacatctgaa tgccgccttc
     2581 aacagctgcc ggacccaaga taaacggtcc gtagatgaag tggtgtaaac cggttgggat
     2641 cagaatacgt tcgaggaagg tgtaaaccca gaccccaagc gcacccgccg aacgcaggaa
     2701 cgcttgcaga gattcaatcc ccgtttgtac ttttggccag ccgagcaatg tcagccaggc
     2761 acaggggatc atcaccagga aggcgataat caccacataa gacgtcccct ggaaaatgcc
     2821 gagaaaaacc ggcagttttt tatcgaacag acggttatgc agcgccgtca caatgccgga
     2881 aatgataatt gcgccgataa tgctggtatc gagggtttta atcccggcca tcattgtcag
     2941 accgctacct gccaccgcgt cctgagtgaa atcgacgccg aagtagcttc cccaggtcat
     3001 tcccatcgcg ttgatgaaat agttccaggt caggaaactc accatcaccg ccagacaagc
     3061 acgtccctgc gcttgcttag caaggccaat gggtaaaccg acagcaaaaa tcagcggcat
     3121 attacggaat accgtccaac cgccctcttc aataatgtgt acgatttgcg cgaataaact
     3181 gttcggatcg gtcagtgatt ccccgacaaa catcgggttt tgcagcaaga tggcaagacc
     3241 caccacaatc ccggcgaagg gaaacagcag cactggcgta aacatcgcgc cgccaaagcg
     3301 ttgaatttga ctgagcattt ccacatcctc ttttctcaat tctgtaagta ggaagcctga
     3361 ttggttatgc ggcaggcctg gagcaaggat aggaagatgc gatcggcaga acatgacgac
     3421 ccaatgggat tcgtgatcgc tttcatgctt ttcgttgggc ggtagagatc tacgttttcg
     3481 cgaaaaagta gacatctgtg agcacaggta cacagcatcg aacacatctt taaaaaaaag
     3541 atgttttttc aatcgattaa gcagaacttg tgtgcgcatt acccgggctt gcaggcaaaa
     3601 aagagatcta gagatgatct acaaaagcat tgcggagcgg ttaagaattc gacttaactc
     3661 cgcagatttc acgctaaaca gccttcttcc gggtgaaaaa aagctggcgg aagagtttgc
     3721 ggtatcgcgg atgaccatcc gtaaagccat tgacctgctg gtagcgtggg ggctggtggt
     3781 ccgccgccac ggtagtggca cttacctggt gcgcaaagat gtactgcatc aaaccgccag
     3841 tctgaccggg ctggtggagg tgttaaaacg gcagggaaaa actgtcacca gccaggtgct
     3901 gatttttgaa atcatgcctg cgcctccggc cattgccagc cagttacgga ttcaaatcaa
     3961 cgagcagatc tacttctccc gtcgcgttcg ttttgtggaa gggaaaccgc tgatgctgga
     4021 agacagctat atgccggtaa aactgttccg taatctttcg ctgcaacatc tggaagggtc
     4081 gaagtttgaa tatattgaac aagagtgcgg gattttgatt ggcggtaatt atgaaagcct
     4141 gacgccggtg ctcgccgata gactgctggc gcggcaaatg aaggtagcgg aacacacgcc
     4201 actgctgcgg atcacctcgt tgtcatatag cgagagcggg gagtttttga attattcagt
     4261 gatgttcaga aatgccagcg aataccaggt ggagtaccat ttacggcgac tccacccgga
     4321 aaagagttaa ccgatactcc agaagagcac cgccagtaat tggggggtga taatgcgcag
     4381 gaacatcact aacggataga cagtggcgta agagagcgcc gccgcaccgc tagttggatg
     4441 aagattatta gcaaacgcca gcgccggagg atcggtcatg gaacctgcca gcatcccgca
     4501 catggtcagg taattcattt tggctaacat ccgcgccaga atgccaacag taatcagcgg
     4561 aacggcggtg atcagggcac cataaccaat ccagcttagc ccttcgccat tgaccagggt
     4621 attcacaaaa tccccaccag atttcagacc aacgaccgag aggaacagca cgatccccag
     4681 ctcccgcagc gcgaggttgg cacttggcgg cataaaccag tacagcttgc cgatactgcc
     4741 gatacgcccg aggatcaacg ccataatcag cagaccgccc gccagcccca gtttcaacgc
     4801 ggccgggaat cccggcacaa agacgggaat agaacctaac aatacgccaa gcccgatgcc
     4861 aataaacact ggcagcatct gaacctgttg cagtttttgc tgcgcattcc ccagcacatt
     4921 ggcaacggca tcaattgcgg acggacgccc caccaggttg aggatatcgc cgaactgcag
     4981 gctgatatcg ccactggcga ccagttcgac cccggcacgg ttcaggcgcg agataacaac
     5041 gtcatagcgt tctttaaagt gcaggtcgcg aatacgtttt ccgagcacgt tttcattggt
     5101 gaccaccaca cgctcgacgc gcaaatcagt gtctttcgtg gacagcgaag tatcgacctc
     5161 ctgaccaatc accagttgcg cattatgtaa atccgctggc tgacccacca gatgcagcaa
     5221 atcgcccagt tggataatgg tatctggcga aggaactttt agggtttctt cgcgtttcag
     5281 acgcgagcag ataattttgt cgccgttgag aatcggtaca tctttaatgg ctaaatcatg
     5341 caggttaggg ttctcaacgc gaatattgat agtcttgatc agcgcgccgc cattggtgcg
     5401 tgaagactcg tgctgctgag cttctgtctc gacattgacg cggaaaataa cccgcaacat
     5461 ccacatggtg aacaaaatcc cgcaaatgcc gaatggatac gccatcgcgt aactcatccc
     5521 catctgatcg accatttcca ttggtgtacc caggtcgcgc aaaatctgct gccctgcccc
     5581 cagcgctggc gtattggtaa ccgcaccgga gaaaatcccc agcactaccg gcagtggaat
     5641 atcaaacagt ttatgcagga tggcggtaac cagaccaccg atgatgacga tcagaacagc
     5701 aaacaggttg aggcgtaatc cggagacgcg caatgaggca aagaagcccg gccctacctg
     5761 aatcccgatg gtataaacga acaggatcag gccaaattcc tgaataacat gcagcatatc
     5821 gctacttaat gtcatccccg cctgagaaac aaaatggccg acgatgatcc caccaaacag
     5881 cacgccgcca atacctaatc ctatgccgcg aaatttgacg ttgccgataa acaaaccgac
     5941 gactgccacc aaagccagaa tactgaccgt taatgctata tcactcatcg tccatttctc
     6001 ttgagataac attttaattg ctacggatta tggctgaatc ggtgaagcag atttgcctta
     6061 tagcgcacat tattacggga aagaacgtgc cgaaatatct taaacagtcg aaatttaacg
     6121 cattgttgaa gaagaaagac aaaaaaagcc ccaccagaat ggcggggcaa agagaatagc
     6181 tagttagcta tttaacgcgg gacgttcgct gatagcgata cgctgcgctg cgatgggttc
     6241 aggctcatta cgaattaaat caatatgcag taaaccgttt acgaaagttg cgccagagac
     6301 ttccatattt tcagccagcg taaagctcag gctaaatggc tgattcataa gcccttgatg
     6361 cagccatttt ttctcttctt ttggctgctc cggcgtgcct tttacgctca ggcgcgtacc
     6421 ttccagttga atctctaaat cttcctgacg gaaacctgcc agcgcaaggg taatgcggta
     6481 gtggttatcg tcgcttttct caatgttgta cggcgggaag ctctgacttt caccggcgtt
     6541 ttgcagtgcg ttggccagtt tgtcaaaacc gatccattga cgcatcagtg gggataaatc
     6601 gaagttacgc atagtcattt ctccttctaa gaagcgagta agtacctgca aatccgaaga
     6661 ttcgcatatg ctccctgacg gcgagcatgg agatgtcagg ccgcgccagg cggccttaag
     6721 gaattagttg atttcgatac ggcgcggttt tttcgcttcc ggaatcacgc gttcgagatc
     6781 gatatacagc aaaccattta ccaggttagc gccacgaaca tgaatgttct cagctaactg
     6841 gaatttgcgt tcaaagttgc gttcagcgat gccctggtac agataggtgc gctctttttg
     6901 ttcgtcggcg tgagcacctt tcaccaccag cagattatcc tgggcggtaa tttccagttc
     6961 gctctcagca aaaccagcca cagcgatagc aatgcggtaa tggttttcgt ctaccagttc
     7021 aacgttatac ggagggtagc cgccattact ctggctctgg ttgttttcta agtggttaaa
     7081 caaacggtca aatccaatag cagaacggta aagcggggat aaatcaaagt tacgcataat
     7141 caatagctcc tgaaatcagc gagaatgtaa gaccttccac aatggacagg tcaggtagcc
     7201 agaacaccca tcaggcgcgt cctcatcgac tacgatgtaa aaatgggtct ggaaatgaac
     7261 ttttcaagcc ttatcagcgg actttttttt gcagtttatg gtctattgca tagactgagg
     7321 gggcagcacg aaacgttaaa gtgcgatagc cgccacagag cgacgtaatg atgatgttat
     7381 tttcagcaag ggatcgctat aactcatcag gccaacaccc acgatcaaca gatgaattta
     7441 ttatgataag aaatgtgttg ttagcgttca tgatatgcag cggaatggca ttactcggag
     7501 gatgctccag cgtgatgtcc cataccggag ggaaagaagg aacgtatccc ggcacccgcg
     7561 ccagcgccac catgattggc gatgatgaaa ccaactgggg cactaagtcg ctggcgattc
     7621 tcgatatgcc gtttaccgct gtgatggaca cgcttttatt accgtgggat gtgttccgca
     7681 aagacagctc ggtgcgctca cgcgttgaaa aaagcgaagc caacgcgcaa gccaccaatg
     7741 cggttattcc accggccaga atgcctgata actgatttag cgatcagttt cggtgatcca
     7801 cagctggcca ccttccatcc acgccagcca ttgaccatcc ggcgagaaga ccacggcgtc
     7861 cgcagaaggt ggattggcgt ggttttcggt taaatactca acctcgccgc tttgcgcatg
     7921 ggcacaagca attcgattat ccagcacaaa gcccaaccat tctcctgacg gatgccagtt
     7981 aaatgcagac tgaatatccg ttttgttatg ggttaactgg cgcggctcgc cgccctgtgg
     8041 cgagataagc cacagttgca caatgccgtt atcatcacgc attaaaaacg cgatttgcgt
     8101 accctgcgga ttacagcgca cccagtggcg cgggacgttg actaaccccg gataagcccg
     8161 atggtgggta aaggttaaac gtcgctgcac gacgccacgc ggtggcgcgg gcagggtggt
     8221 ttccgttcca cttaacggcg catcacctgc cgctttccag ccagcttcat cttgcggcaa
     8281 ctcaacgata aacagctccg gcactttctc accctttggc gaaagtgtgt cgccaataaa
     8341 cgccagcgcg tgatttccta cccatccttc ttcataagca cgattgattt catcactgcc
     8401 aggctgcggc gtgggcgtgg ttttactcac cagcacacac cagtggctgc cgctgtattc
     8461 acgcggatgc tgcttttgta cgttgaccgg accaaacggc gcagcaacac cgacgtttcg
     8521 caaatccagc gccggatcga gttcatgcat tacatggtcg ttataggtaa agctcaccct
     8581 ttcaccgttc gggctaaaga catgcacatg gctgccgccg cgcagcgctc ctggcgtata
     8641 cggcgcggta atatccattg catcgagatt gctcaccttg ccgccttcag caatgactcc
     8701 gcgccgatga tggaagtcgt aatgccatgt ttcatcagga ttttccgggc cgtgaataaa
     8761 gacatatttc tctgacttcg gatgaacggt caccacgccg acgtgtgcgc cctgtgacgc
     8821 acagtagata acctcgacct caccggtatg gatattcaca cgctcaatgg tctcgccggt
     8881 aaacgacgcc ccagaaggac gcacgtcaaa taccagccac tggctgtcgg gcgtccaggt
     8941 attggtattg gtgagcaggt gattacgggg agcaaaggtt atctgtttca tggcgcggtc
     9001 ctgatacaaa acgggtccag ccatcatact tcacaaggac ttcactttca gacaatagtc
     9061 ttttgccatc gtgatgaatg tgagtgaaaa gatggaacat ttcgacgtgg cgattattgg
     9121 cctcggcccg gcagggtcgg cgttggcacg aaagttagcc ggcaaaatgc aggtgatcgc
     9181 gctggataaa aagcaccagc atggtactga aggtttcagc aaaccttgtg gcggtctgct
     9241 ggcaccggac gcgcagcgat cttttattcg cgatgggctg acgcttcctg tcgatgtgat
     9301 cgctaatccg cagattttca gcgtcaaaac cgtcgacgtc gccgcatcgc tcacgcgtaa
     9361 ctaccagcga agctatatca atattaatcg ccatgctttc gacttgtgga tgaaatcgct
     9421 gatccccgcc agcgttgagg tttatcacga cagcctgtgt cgaaaaatct ggcgtgagga
     9481 tgataaatgg catgtcattt ttcgtgcaga cggctgggag cagcatatta ccgcccgcta
     9541 tctggtcggt gccgatggtg ccaactcgat ggtgcggcga catctctacc cggatcatca
     9601 aatccgtaaa tatgtcgcaa tccagcagtg gttcgcagag aaacatccgg tgccgttcta
     9661 ctcctgcatc tttgataatg cgataactga ctgttattca tggagtatca gcaaagacgg
     9721 ttattttatc tttggcggtg cctatccaat gaaagacggt cagacgcgtt tcacgactct
     9781 gaaagagaaa atgagcgcct ttcagttcca gtttggcaaa gcggtgaaaa gcgaaaaatg
     9841 cacggtgctg tttccctcac gctggcagga ttttgtctgc ggtaaggaca acgcctttct
     9901 gattggtgaa gcggcgggat ttatcagcgc cagctcgctg gaggggatta gctatgcgct
     9961 ggatagcgca gacattctgc gttcggtgtt actgaagcag ccagagaagc tcaataccgc
    10021 ttactggcgc gccacccgca aactgcgttt aaaactcttc ggcaagatag taaaaagccg
    10081 atgcctgacc gcaccggctt taagaaagtg gattatgcgc agtggtgtgg cacatattcc
    10141 acagttgaag gattagccaa cgcgcttcac atcgcccacc agcaggatat aagagagcgc
    10201 gccaatcaac gcgacggcgg agatataaac cagtgcaggt gcgaaaccgt aaccctgcgc
    10261 caggtacccc accaccagcg gaacggtgat gccgcccaga ccaccggcga agttaaacac
    10321 gccgccggtt aaaccaatca ggcgcatcgg tgccagagaa gaaaccagcg accaggtaat
    10381 cgaagcaaaa ccgttaccga agaatgccag cgccatcagg cacataatca tcatcggatc
    10441 gttagtgtag ttagcgccca taatgcaggt ggagatcagc aagccgcaga taatcggcgt
    10501 tttacgcgca aagcccagtg aaaagccctt acgtaccagc agatccgcta cccagccaga
    10561 gagcaggacg ccgacaaacg ccgcgaggaa tggcaccgtg gtcataaagc ctgctttcag
    10621 cgccgtgatt cctttttcct gggttaaata gttcgggaac caggttaaga aaaaccacag
    10681 tgtagaagcc accgcaaatt gcccaagata gacgccgatc agtttacgat ggaacaccag
    10741 tttccagtct ttggctgtta acggctgacg tgcctctttc ttcactggcg catcaccatc
    10801 caccagaccg ccgccatcac gaatgtaatc cagttcagct ttgctgatgc ctttggtcag
    10861 gcgcggcggc tgataaacct taaaccaaat cagcgaccag ataatgccga taccaccagt
    10921 cacaatgaac acccagtgcc agctcaacat ctcctgaatc cagatcagca gcggagtcaa
    10981 aaacgccaga ccgacaaact gaccagacgt ataaaaacca acggcggaag cgcgttcatg
    11041 ttccgggaac cagctggtca ccatccggtt attggtcggg aaagcgggcg cttcgaaaat
    11101 accggttatc gcacgcagac caattaatga cattaatccc gtggcaaagc cctggaataa
    11161 agtcgccact gaccagccaa atatcgcaat aaaataagtc acgcgagaac ctacgcgatc
    11221 taaaaaccaa ccgccgggga tctggcatag cgtataaagc caggcgaagg ccgaaaatac
    11281 atagcccatt tccgctttcg taatgccgaa ctcttcctga atatgggcgg aagccacggc
    11341 caggttagcg cggtcaacat aacaaatgac caccgtaata aagatcatca ccagcgtcag
    11401 ataacgccga cgccccggct ttgctgcatt aacgggaata tccatagcga gctttctcca
    11461 gattttgggc atagcgaagc cgctcaccat gccctgtaat ttacagaggg ttatattttt
    11521 gtattgctgt tttagtgccc gatgaggggc ttacgtggca ggaattacca ctctgctacg
    11581 ctgttatctt catgacgcca gagtggatta cgccagtccg gggcattttt actgaactca
    11641 ataactttag cttcgtcgat ttccacgcct aagcccggtt tcgttagcgg tttaaagaag
    11701 ccgccaacca tgctgaagtc ttctttgttt ttcacaaagt cgagtaactc cgcgcctttg
    11761 ttgtaatgaa ttcccatact ttgttcctga agtaccgcgt tataggaaac aaagtcgata
    11821 tgcagacaag ccgccagtgc aatcggtccg agcggacagt gcggcgcaag ggtcacgtca
    11881 taggcttctg ccattccggc aattttgtag cattcggtaa taccgcctgc atgggagaga
    11941 tccggttgta gaatcgaaat accgcctgcc tccagcacgc gtttaaaatc gaaacgtgag
    12001 aacatgcgct cacccgccgc cagtggaata tgcgtttgtg ccgccaattt cgggtagtat
    12061 tcggcttgtt ccgccagcac cggctcttca ataaacagcg ggcgatacgg ctccagttct
    12121 ttaatcagca ctttcgccat tggcgcgctg acgcgaccgt ggaaatcaag accaaactca
    12181 atctgattgc caaaagcttc acgaatttgt gccacggtgt tgactgccgc atctaccgcg
    12241 cgggagttat caattagccc cagttcttca caaccgttca gtttgaaggt atcgaagccg
    12301 atttcgcgca gggttttaat accgtcgatc acatccgccg gacgatcgcc gccgacccaa
    12361 ctgtaggctt taattttgtc gcgaaccagg ccgcccatca gttgccagac cgacgcattc
    12421 agcactttac ctttgatatc ccataacgcc tggtcgatcc cggcaatggc gctcatcagg
    12481 attggaccgc cacgataaaa tccggcgcga tacatcactt gccataagtc attgatgcgc
    12541 tatggatcct gaccaatcaa atagtcaccc agctcgtgaa ctgccgcttc caccgtgcgg
    12601 gcgcggcctt caatcactgg ctcgccccag ccgacgacgc cttcatcggt ttcaattttc
    12661 aggaacatcc agcggggagg taaacgatac gtggtaattt tggtgatttt cattgcactg
    12721 cctctcgata cgccttaaca aatgctgctg cctgctgcgc ggtgcgttct acggactgcc
    12781 cggcgcgata gagatcgctg cctaagcccg ccccagcaca acctgcgtct atccactgcg
    12841 ccaggttttc tggcgtcacg ccgccaacgg caaagactgc gatgtccgat ggcaataccg
    12901 cttttaacgc tttgatgtat tgcggaccaa aagccgatga cggaaatatt ttcagcgcct
    12961 gcgcgcccgc ttcgagcgcg gtaaaggctt cggtcgccgt cgcgcagccg gggcagacgg
    13021 tcatgccgta gcccaccgca cggcggatca cttcactatg gatattgggc gtaacgatga
    13081 gctgacagcc catcctggcg agcgcatcga cctgttcagg tttcagtacc gtacctgcgc
    13141 caatcaacgc cttgtcgcca tacgcatcaa cgatggcggg aatgctttgc tcccattgtg
    13201 gggaattcag cgggatttca accgcgtcga acccggcgtc aatcaccgcg ccaacatgcg
    13261 ccagcgcctc gtcgggctta atgccgcgca aaatggcgat cagcggaagt ttagtttgcc
    13321 actgcatgag cgatgctcct tataccagcc tgaaatgccg tgtcgcccgc caccgccgtc
    13381 acgtcgcaac ccatcgcctg aaaggcttgc tggtagcgcg cggtcaacga tgttccggcg
    13441 acaagggtga tggcgtgttg atgggccaca tagtcgcgca tactggcgac ctctgcgcca
    13501 atcaacaaac cagagagaaa ttcactgacc tgttcacggg gaagtgttcc cagcacatgc
    13561 gaggcacgaa cttcaaaaag ctgcggcaat atggcgggcg tattaagacc acgctcaagg
    13621 ccagccgtga aggcatcggc agagttttcc tgcggcggca aacctgcacc aatcaatgag
    13681 tgatttaaca gtaaatgatg taattcaccg gtcatcacgg tgcgaaaatc gttgatttgc
    13741 tggctatcgg cctgcaccca tttgcaatgc gtaccgggca tgacataaag agaggaagga
    13801 gccagagctc gcgcgccgat caattgagtt tcttcgccgc gcatcacatt gtggttatca
    13861 tcatgagaga cgcataatcc gggaataatc cagatattgt cgccaacaga cgttaattgt
    13921 tcgccaatag acgaaaaacg ggcaggaaca gataaatacg gagcaacttt ccagccgaca
    13981 ttgctgccga ccattcctgc cattaccact ggcgtattct cttcacgcca gtcggtcgtg
    14041 acttctgcta acaccgcagc cggagatttt ccgttcaggc gcgtgacgcc tgcttctgat
    14101 tgcctgctct ccaggcagtg gtcgccctga taaagccagg cgcgcagatt ggtcgatccc
    14161 cagtcaattg cgatgtagcg agctgtcatg tgatttcctt taaccttcgt gtcgagctgg
    14221 cgatcatggt aagcgccgcc tgctctgccg catcgccgtc ctgatgccgt atcgcatcga
    14281 acagcgcctt atgttcctgg agcgtttgcg gcatgttggc ctcatcgccc atccaggttc
    14341 gttcaaaaac cgcccgctgc agcgaactga tcgcaatgct aagttgctgt aacaccgggt
    14401 tatgcaccga ctgcagcacc gcctcgtggt agcgaatatc cgcttcgtta aacgcttcgc
    14461 ggtcctgatt gttggcaatc atctcgttca gcgccgattc aatctgcgcc agatcgctgg
    14521 aggtcgcgcg ctctgctgcc caacgggcaa tcgccggttc caccagattt cgcacttcgc
    14581 tcatggcact gataagccgt gggtcgtagt cgttttccag cacccattgc agtacgtcag
    14641 tgtcgaggta attccactgg ttacgcggtg ccacaaacgc gccgcgataa cgtttcattt
    14701 caatcagccg tttcgccatc aacgaacgga acacctcacg gatgatgttg cgcgaggttg
    14761 caaactcctc acagagttcc gcctcagccg gaagcggcga gcctggtacg tatttgccgt
    14821 gaacgatctg tttacccagc gtaatgacga tgcgatcggt tttattgaga gtcatggaga
    14881 gtccttgtgc tctgtatgtt cttctctact ttaccccgat cgatgcataa cacggcaact
    14941 ttgtagtacc agcgtgatga cgttcgcgtt tgctgtgcgt gtaatgtagt acaaacttat
    15001 attgttgtac tacaatttag atcacaaaaa gaacaatgcg taaaaaaatg acatgcgtcg
    15061 ggcagaaatc tgaaaaggga tatcaggcgc taaacaggag ggaaagaaga gtatgctttc
    15121 aacggcttag ctactcgttt aaaggattaa tcatgaagtt gaatcttaag ggatttttta
    15181 agactgccgg tttattccca ctggcgctga tgctttcggg ctgtatctcg tatgccctgg
    15241 tttcccatac cgcaaagggt agttcaggaa agtatcaatc gcagtcagac accatcacgg
    15301 ggctatcgca ggcaaaggat agtaatggaa caaaaggcta tgtttttgta ggggaatcgc
    15361 tggattacct tatcacagat ggtgccgatg acatcgttaa gatgctcaag gatccagctc
    15421 ttaaccggca caatattcag gttgctgatg acgcaagatt tgttttaaat gcggggaaaa
    15481 agaaatttac cggcacaata tcactctatt acaactggag cacagaagaa gaaaaagcac
    15541 gagtgacaca atatcgtttt gcttgtggtg ttcagcactg tacccggaca gtagaaaacc
    15601 tggaaggtac tatccatgag aaaaataaaa acatggatta ctcaaacgta atggctttct
    15661 accatccatt taaagtgcga ttttatgaat accattcacc cagaggcatt ccggatggtg
    15721 tttccgcagc attactgcca gtgactgtta cgctggacat cattactgca ccgctgcaat
    15781 ttctggttgt atatgcagta aaccaataat cagtaagcgg gcaaacgcgt ttatgctgtt
    15841 tgcccgccca cagattaatt cagcacatac ttctcaatag caaacgccac gccatcttca
    15901 aggttagatt tggtgacaaa gttcgccact tctttcaccg aaggaatagc gttatccatc
    15961 gccacaccga cgcctgcata ttcaatcatt gcgatgtcgt tttcctgatc gccaatcgcc
    16021 atgatttctt ccggtttaat acctaacacg tcggccagtg atttcacacc cgtacctttg
    16081 ttaacgcgtt tatcgaggat ttcgaggaag tacggcgcac ttttcagcac ggtatatttc
    16141 tctttcactt cctgcggaat acgcgcgata gcctggtcga ggatggcggg ttcatcaatc
    16201 atcatcactt tcaggaactg ggtattgggg tccattttct ccgcttcgca gaacaccagc
    16261 ggaatagtgg caacgaaaga ttcatgcact gtgtagtagc tgatatcgcg gttggcggtg
    16321 tacagcgtgg tgcggtccag ggcgtggaaa tgagaaccga cttcgcgaga gagtttttcc
    16381 aggaaacgat agtcgtcata gctgagagca gtttgcgcca cggtgctacc atcagcggcc
    16441 ttctgtacta gcgcgccgtt ataagtaatg cagtagtcgc ccggctgttc catatgcagc
    16501 tctttcaggt agttgtgcac acctgcatac gggcgacccg tcgttagcac gacattcacg
    16561 ccacgggcgc gagctgcggc aatcgcattt ttaacggcgg gtgaaatggt gtgatcgggc
    16621 agcagaaggg tgccatccat atcgatagca atgagtttga tagccatgag ttccccaggt
    16681 agattggttc ctgacccatg ctaacgcgat tccgctcaaa aatcagtaca acacccgagg
    16741 gaaaaggggg atgcaacgcg ggttgcatgc tccccatttg cttagcggaa gagtttccct
    16801 ttcagcagtt ccatacctgc ggaaagcaga tcgttattgg cttgtggtga cacttcacct
    16861 tgcggtgaga gcgcatcaat aatcttcggc aattgctctg ccagtaaact ggaagctgta
    16921 ctggtatcca cgccaagttt ttgcccgaga tcggacaccg catttgtgcc gagcgccgat
    16981 tccagttgct cgccactaac cgattgattg ccctgttgat tactcagcca ggttgagaga
    17041 atggccccta agccgccact ttgcagtttt tccagcagca cctgaatgcc gccctgctcc
    17101 tcaacccaac ttaaaatagc ctgatatttc cccgcatcgc ctttcagaaa ggcaccgaca
    17161 acttcatcaa aaagccccat gataatcacc tgtaaagcgt tacgtgttga cccaaaaagt
    17221 atagatttgc gggatgataa ttgcggattg cagaaataaa aagggcggag atgatctccg
    17281 cccttttctt atagcttctt gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc
    17341 atgaaaattc aatacattgc aagattttcg taggcctgat aagcgtagcg catcaggcac
    17401 gctcgcatgg ttagcgccat taaatatcga tattcgccgc tttcagggcg ttctcttcaa
    17461 taaacgcacg gcgcggttca acggcgtcgc ccatcagcgt ggtgaacaac tggtcggcag
    17521 caatcgcatc tttaacggta acgcgcagca tacgacggct ttccgggtcc atggtggttt
    17581 cccacagctg ttccgggttc atctcgccca gacctttata acgctggatg gagaggccgc
    17641 gacgggactc tttcaccagc cagtccagcg cctgctcgaa gctggctacc ggctgacgac
    17701 gctcgccacg ttcgataaac gcatcttctt ccagcaagcc acgcagtttc tcacccagcg
    17761 tgcagatacg acgatattcg ccaccggtga taaactcgtg atccagcgga tagtcagtat
    17821 ccacaccgtg ggtacgcacg cgaacaatcg gttcaaacag gttttgttcg gcattggtgt
    17881 ggacatcaaa cttccactgg ctgccgtgct gttctttgtc gttcagttcg ctgaccagcg
    17941 cgttcaccca gcgggtaacg gtctgctcat cagagaggtc ggcttccgtc agcgtcggct
    18001 gatagataag ctctttcagc attgctttcg gataacgacg ctccatgcgg ttgatcattt
    18061 tctgcgtcgc gttgtactca gacaccaatt tctctaacgc ttcgccagcc aatgccggtg
    18121 cgctggcgtt ggtgtgcagc gttgcgccat ccagcgcgat agagatctgg tactgatcca
    18181 tcgcttcgtc gtctttaatg tactgttcct gcttgccttt cttcactttg tacagcggcg
    18241 gctgagcgat gtagacgtga ccgcgttcaa cgatttccgg catctgacga tagaagaagg
    18301 tcaacagcag cgtacgaatg tgcgagccgt cgacgtccgc atcggtcatg atgatgatgc
    18361 tgtgataacg cagtttgtcc gggttgtact cgtcacgacc gataccacag ccaagcgcgg
    18421 tgataagcgt cgccacttcc tgagaagaga gcatcttatc gaagcgcgct ttctcaacgt
    18481 tgaggatttt acccttcagc ggcagaatcg cctggttctt gcggttacgc ccttgcttcg
    18541 cagagccgcc cgcggagtcc ccttccacca ggtacagttc ggaaagcgcc ggatcgcgtt
    18601 cctggcagtc tgccagtttg cccggcaggc ccgctaagtc gagcgcacct ttacggcggg
    18661 tcatttcacg cgcgcgacgc gccgcttcac gggcacgggc agcatcgata attttgccaa
    18721 ccacgatttt cgcgtcggtt gggttttcca gcaggtattc tgccagcagt tcgttcatct
    18781 gctgttcaac cgctgatttc acctcagaag aaaccagttt gtctttggtc tgggaggaga
    18841 atttcgggtc cggcactttc acggaaacga ccgcaatcag gccttcacgc gcatcgtcac
    18901 cggtggcgct aactttggct tttttgctgt agccttcttt gtccatgtag gcgttcaggg
    18961 tacgggtcat cgccgcacgg aagcctgcca ggtgagtacc gccgtcacgc tgcggaatgt
    19021 tgttggtaaa gcagtagatg ttttcctgga agccatcgtt ccactgcaac gccacttcga
    19081 cgccaatacc gtctttttca gtggagaagt agaagatatt cgggtggatc ggcgttttgt
    19141 tcttgttcag atattcaacg aacgccttga tgccgccttc atagtggaag tggtcttctt
    19201 tgccgtcgcg cttgtcgcgc agacgaatgg aaacgccgga gttgaggaac gacaactcac
    19261 gcagacgttt cgccagaatt tcatattcga actcggtcac attggtgaag gtttcgaggc
    19321 tgggccagaa acgcaccatg gtgccggttt tttcagtctc gccggtaacc gccaacggtg
    19381 cctgcggtac accgtgttcg tagatctgac ggtgaatttt accctcgcgc tggataacca
    19441 gctccagttt ttgcgacagg gcgtttacta ccgaaacacc aacgccgtgc agaccgccgg
    19501 acactttata ggagttatcg tcaaatttac cgcctgcgtg cagaacggtc atgatcactt
    19561 ccgccgccga tacgccctct tccgggtgaa taccggtcgg aatgccgcgc ccgtcatcct
    19621 gtacagagac agagttatcg gcgtgaatgg tgacgataat ttctttacag tgacccgcga
    19681 gcgcttcgtc gatagcgtta tctaccacct cgaataccat gtggtgcaga ccggtgccgt
    19741 catccgtgtc gccgatatac atacccgggc gcttacgcac cgcatccagc cctttcagga
    19801 ctttgatact ggaggagtca taagaattcg acatcaacgt ttctcgctca tttatacttg
    19861 ggttaatccg ttattttacc cttttccacg gtaaacatct tcgaattttc gtccgacatg
    19921 tctataacgt gttcagcact gatcgcgctg acaaagacct gtgattgcgt cgcttttaag
    19981 cggctggcaa gcagcccgcg acgctcatca tcaagctcag aggcaaaatc atctatcagg
    20041 tagagacacc gccgcccgct ttcacgggtg aggaactctc cttgcgccag acgtaaggcg
    20101 cacatcaaca gcttaagctg cccacgcgat aaggtatctt ccaccggcgc accgtcggcg
    20161 cgaatgcgta agtccgcttt atgcgggcca tgcgcggtgt aggttagctg gcgatcgcgt
    20221 tcaaaattac gttccagcac ctcagcatat tctgtctctt tctcccagcc gcgctggaaa
    20281 gagaaagtca gagaaaactc agggagaaat tgcttacaag tatcggccat atcagccgcg
    20341 ataccggcgc tatactccgc gcgccaggtg ctgatttgct ccgccagcgg gatcagctct
    20401 ttatcccacg ggcgtagctg ttcgtaacgt gtcacctggc gcagcgccgc attgcgctgc
    20461 ttgagcaatc gcttgagatt gctccaggcg gtgaaaaatc cgggttcgtt gtgaaagcat
    20521 ccccagtcga ggaatgctct tctgtatttg gggccgccgt tgagtaaagt aaacccttct
    20581 ggcgttatca actgcattgg catcaggtgc gccagttccg cgaccttatg cccgtctgta
    20641 ccgtcgatgc ggactttgct gtcgccctgt ttgtctttgg ttaagccaat cgctgtctcg
    20701 cgctcttcgc cctgtaatcg cccgtggaga acaaacgcct cctgctcatg gcgaatgacg
    20761 cgaccaatct gcaaactgcg aaacgcccga ccatggccga gcgtatagat ggcttccagc
    20821 acgctggttt tgccactgcc gttggcacct accagaaagt taaagccggg agataaagcg
    20881 agatccgcgg tttcaatgtt gcggaaatcg cggatcaaca agcgggtgag ggacattaca
    20941 gtctcattgg catgacaaca taggcagccg actgtgatgc ggcatcttca atctgcacgc
    21001 tggaaaccga atcggtcagc atcatgcgga cgttttcaca tttcagcgcg ttcagaacat
    21061 ccagcacgta gctgacgttg aagccgattt ccatctccgc accgctatag gtaacgtcga
    21121 ggatctcttc cgcttcttcc tgttccgggt tgttggcggt gattttcagc tggttttcgc
    21181 tgacataaag acgtacgccg cggaatttct cgttagagag aatcgccgcg cgagcaaacg
    21241 cctgcttgag cagatcgcag ccagcttcca gatgtttgtc cgggttcttc ggcagaacgc
    21301 ggcgataatc cgggaagcga ccatccacca gtttggaggt gaagataaag tcgccaacgt
    21361 gggcgcgaat gttgttgctg ccaatctgta cgcgcagcgg attgtcgccg ccgtcgagca
    21421 tacgcatcag ttcaatcacg cctttacgcg gcacgatcac cgaatggctt ggcaaagatt
    21481 gaccaattgg cattgaacag actgccagac ggtggccgtc ggtcgccacg gtgcgcagtt
    21541 cttcgccttc ggtttcaaac agcataccgt ttaagtaata gcgaacgtcc tgatgcgcca
    21601 tagaaaactg ggtcgcttca atcagacgct tcatcgtcgc ctgcggcagg gtaaattcga
    21661 cttcgctctg ccagtcatcg aggttcggga aatccgccgc tggcagggta gacagcgaaa
    21721 aacggctacg cccggagcgc accagcatcc gttcaccttc cagctgtacg gcaatttccg
    21781 cgccttcagg cagaccacgg cagatatcaa agaatttgcg cgccggaacg gtcgtcgctc
    21841 ctggctcgtg tggctgaacc agcgcaacgc gtgccaccat ttccatctcg agatcagtac
    21901 cggtcagcga caacgtaccg tcagcaacct gtaacagcag attaccgaga atcggtagcg
    21961 taggacgacc acctaacggc ccgctcacct gttgtagcgg ttttaataaa tgctcacgtt
    22021 ctacggtaaa tttcataggt ttacgatgac aatgttctga ttaaatttga aaaatcttct
    22081 ttgatatcgt ggctctcttc acgcaactgc tcgatcttac ggcaggcatg aagcaccgtc
    22141 gtgtggtcac gaccaccaaa cgcatcgcca atctccggca gactgtggtt agtcagctct
    22201 ttcgccagcg ccatcgccat ctggcgcgga cgcgccaccg agcgggatcg acgcttggaa
    22261 aggagatccg cgactttgat cttgtagtac tccgccaccg tcttctgaat attgtcgatg
    22321 gtgaccagtt tttcctgcaa tgccagcaag tcgcgcagtg cctcacgcac gaagtcgatg
    22381 gtgatcgccc gtccggtaaa gttggcgtta gcaataacgc ggttcagcgc cccttccagc
    22441 tcacgtacgt tagatcgtag acgcttggcg ataaagaacg ccacttcgcc tggcaaacga
    22501 atgtcatttt cgtcggcctt tttcatcagg atcgccacgc gggtttccag ctctggcggt
    22561 tcgatcgcca cagtcagtcc ccaaccgaag cgggatttca aacgatcctc aacgccgttg
    22621 atctctttcg gatagcgatc cgaggtgaga atgatctgtt gattaccttc cagcagggcg
    22681 ttgaaggtgt ggaaaaactc ttcctgagat cgttctttat tagcaaaaaa ctgaatatcg
    22741 tcgatcagca gtgcatctac ggaacggtag tagcgtttaa actcttcgat cgcgttgttt
    22801 tgcagggctt taaccatgtc ctgaacaaag cgctcggagt gcatataaac cactttggca
    22861 ttcggcttgc gcgccataat gccgttaccc accgcatgca gcaggtgagt tttacccaga
    22921 cccgtgccgc cataaaggaa caatgggtta taggcaccgc cagggttatc cgccacctgg
    22981 cgagccgccg cgcgcgccag ttggttagat ttaccttcaa cgaagttatc aaacgtgtgt
    23041 ttgacgttta cgttagaacg ataggtcggt tctgccgggg ccgggacgtt atcccaacct
    23101 gagcgcgtag aaggcgcagc acgttgcggc tgcgtttgcg ccacctgtgc aggggccgcg
    23161 acgttgctcg tcactgccgc ttgtggcgtt tgcgtcaccg gtttggtgcc gacttcaaaa
    23221 cgcagctgtg gggcatccgc tccgcagaaa ctggttagca gtccattgat attattaagg
    23281 tacttgtccc gtacccaatc gaggacaaaa cggtttggcg cgtacagggc cagcgtgtta
    23341 tcgctcagtt ccgcctgcaa tgggcgtatc cacatactga attctgtggc tggtaactca
    23401 tcctgcaatc gggcaagaca ctgctgccaa agcgaaagtg acacggcgga ctccactcga
    23461 acaaaagtcg ataatgacta aggctgaaac attcatgatt gttgacgtac gtcgaaaaga
    23521 ccctgcttga gggtgacgca cgaaccgctg tctgcggtta tatgcccgat caagatcctg
    23581 caaaacgatc gggaccgcgg atcatagcct aaactgcgca agagatcttc tgtttctcac
    23641 agattttccc gatttatcca caggactttc cagacctcgc taagtgtaaa cgatcctgcc
    23701 gcgaggcggg cacgatttac gccgcatatt ggaaaattta atgaccatag acaaaaattg
    23761 gcttaatcga tctaataaag atctaggacg atccttgcgc tttacccatc agcccgtata
    23821 atcctccacc cggcgcgcca tgctggtttt cactggtgtg aggtcgtaca ttttccctgc
    23881 gaaaaggtgc ggaaaagcgc ggtaaataag gaaagagaat tgactccgga gtgtacaatt
    23941 attacaatcc ggcctcttta atcacccatg gcttcggtgt ccatcgtttc atttttcggc
    24001 ggatatccaa taaagccatt gaatttattc aagtttaggt agaaatcgcc atgaaacgca
    24061 cttttcaacc gtctgtactg aagcgcaacc gttctcacgg cttccgtgct cgtatggcta
    24121 ctaaaaatgg tcgtcaggtt ctggcacgtc gtcgtgctaa aggccgcgct cgtctgaccg
    24181 tttctaagta ataaagctaa cccctgagtg gttaagctcg catttcccag ggagttacgc
    24241 ttgttaactc ccagtcaatt cacattcgtc ttccagcagc cacaacgggc tggcacgccg
    24301 caaattacca ttctcggccg cctgaattcg ctggggcatc cccgtatcgg tcttacagtc
    24361 gccaagaaaa acgttcgacg cgcccatgaa cgcaatcgga ttaaacgtct gacgcgtgaa
    24421 agcttccgtc tgcgccaaca tgaactcccg gctatggatt tcgtggtggt ggcgaaaaaa
    24481 ggggttgccg acctcgataa ccgtgctctc tcggaagcgt tggaaaaatt atggcgccgc
    24541 cactgtcgcc tggctcgcgg gtcctgatag ccctcattcg ggtctatcaa cgcctgatta
    24601 gtccgctact cgggccgcat tgtcgtttca ctccaacctg ttcaagctac ggaattgagg
    24661 cattgcgcag gtttggagtg ataaaaggca gttggttgac ggtgaaacgc gtattaaaat
    24721 gccacccttt acaccctggt ggtgacgatc ccgtcccgcc cggaccattt gataccagag
    24781 aacactaacg atggattcgc aacgcaatct tttagtcatc gctttgctgt tcgtgtcttt
    24841 catgatctgg caagcctggg agcaggataa aaacccgcaa cctcaggccc aacagaccac
    24901 gcagacaacg accaccgcag cgggtagcgc cgccgaccag ggcgtaccgg ccagtggcca
    24961 ggggaaactg atctcggtta agaccgacgt gcttgatctg accatcaaca cccgtggtgg
    25021 tgatgttgag caagctctgc tgcctgctta cccgaaagag ctgaactcta cccagccgtt
    25081 ccagctgctg gaaacttcac cgcagtttat ttatcaggca cagagcggtc tgaccggtcg
    25141 tgatggcccg gataacccgg ctaacggccc gcgtccgctg tataacgttg aaaaagacgc
    25201 ttatgtgctg gctgaaggtc aaaacgaact gcaggtgccg atgacgtata ccgacgcggc
    25261 aggcaacacg tttaccaaaa cgtttgtcct gaaacgtggt gattacgctg tcaacgtcaa
    25321 ctacaacgtg cagaacgctg gcgagaaacc gctggaaatc tccacctttg gtcagttgaa
    25381 gcaatccatc actctgccac cgcatctcga taccggaagc agcaacttcg cactgcacac
    25441 cttccgcggc gcggcgtact ccacgcctga cgagaagtac gagaaataca agttcgatac
    25501 cattgccgat aacgaaaacc tgaacatctc ttcgaaaggt ggttgggtgg caatgctgca
    25561 acagtatttc gcgacggcgt ggatcccgca taacgacggt accaacaact tctataccgc
    25621 taatctgggt aacggcatcg ccgctatcgg ctataaatct cagccggtac tggttcagcc
    25681 tggtcagact ggcgcgatga acagcaccct gtgggttggc ccggaaatcc aggacaaaat
    25741 ggcagctgtt gctccgcacc tggatctgac cgttgattac ggttggttgt ggttcatctc
    25801 tcagccgctg ttcaaactgc tgaaatggat ccatagcttt gtgggtaact ggggcttctc
    25861 cattatcatc atcaccttta tcgttcgtgg catcatgtac ccgctgacca aagcgcagta
    25921 cacctccatg gcgaagatgc gtatgctgca gccgaagatt caggcaatgc gtgagcgtct
    25981 gggcgatgac aaacagcgta tcagccagga aatgatggcg ctgtacaaag ctgagaaggt
    26041 taacccgctg ggcggctgct tcccgctgct gatccagatg ccaatcttcc tggcgttgta
    26101 ctacatgctg atgggttccg ttgaactgcg tcaggcaccg tttgcactgt ggatccacga
    26161 cctgtcggca caggacccgt actacatcct gccgatcctg atgggcgtaa cgatgttctt
    26221 cattcagaag atgtcgccga ccacagtgac cgacccgatg cagcagaaga tcatgacctt
    26281 tatgccggtc atcttcaccg tgttcttcct gtggttcccg tcaggtctgg tgctgtacta
    26341 tatcgtcagc aacctggtaa ccattattca gcagcagctg atttaccgtg gtctggaaaa
    26401 acgtggcctg catagccgcg agaagaaaaa atcctgattc ggtgagtttt cgctaaaata
    26461 agggcggtca gttgaccgcc ttttttcttt tcgtagggtg gataagcacc gcgcatccgc
    26521 cacacaaagc aacaggaaca tcatgagcga taatgacact atcgtagccc aggccacgcc
    26581 tccgggacgt ggcggcgttg gcatcctgcg catctccggc ctaaaagccc gtgaagttgc
    26641 cgaaaccgtg ctgggtaaac tgcctaagcc gcgctacgcc gattatcttc cgtttaaaga
    26701 cgccgacggc agcgtgctcg atcaggggat tgcgctatgg ttccctggcc cgaactcgtt
    26761 caccggcgaa gatgtgctgg aactgcaggg tcatggcggt ccggtgatcc tcgacctgct
    26821 gttaaaacgc attctgacca ttcccggcct gcggattgct cgccctggtg agttttccga
    26881 acgcgcgttt cttaacgata aactcgactt agcccaggcc gaggcgattg ccgatcttat
    26941 cgacgccagt tcggaacagg cggcccgttc ggcacttaac tcgctgcaag gcgcattctc
    27001 cgcacgggtc aaccatctgg tggaagccct cacccacttg cgcatttacg tcgaagcggc
    27061 aattgatttc cctgatgaag agatcgattt cctctccgac ggcaaaattg aagcccagct
    27121 taatgacgtg attgccgatc tcgatgcagt gcgtgctgaa gcacgtcagg gtagtttgtt
    27181 gcgcgaaggg atgaaggtgg tgattgccgg acgtcctaac gccggtaaat cgagcctgtt
    27241 aaacgcgctg gcgggccgtg aagcagcaat cgtaactgat atcgccggaa ccacacgtga
    27301 cgtgctgcgt gagcatatcc acattgacgg aatgccgctg catatcatcg ataccgctgg
    27361 gctacgtgaa gccagtgacg aagtggaacg tattggtatc gagcgcgcgt ggcaggaaat
    27421 tgaacaggcc gaccgcgtgc tgtttatggt cgatggcacc acaacagacg ccgtggatcc
    27481 ggcagagatc tggccggaat ttatcgcccg tctgccagcg aaactaccga tcaccgtggt
    27541 gcgcaataaa gccgatatca ccggcgaaac gctgggaatg agtgaagtga acggtcacgc
    27601 gttaattcgt ctctcggcaa ggacaggtga aggcgtggag gtgctgcgta accatctcaa
    27661 acagagcatg ggctttgaca ccaacatgga aggcggcttc ctggcgcgtc gtcgccacct
    27721 acaggcgctg gaacaggcag cggagcattt gcagcaaggt aaagcgcaac tgttaggtgc
    27781 ctgggcgggt gaactgctgg cggaagagtt gcgcctggca cagcagaact taagcgaaat
    27841 caccggggaa tttacctctg atgatttgct ggggcggatt ttctccagct tctgtattgg
    27901 taagtaaccg cgcttacgaa gccgtattct gactgtcaga tgcggcttcg cttcattgtt
    27961 agcactcctg ttattcctca accctttttt taaacattaa aattcttacg taatttataa
    28021 tctttaaaaa aagcatttaa tattgctccc
//
LOCUS       DAZPLI010000040        27908 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZPLI010000040 DAZPLI010000000
VERSION     DAZPLI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.040"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            408..2429
                     /gene="uvrB"
                     /locus_tag="ACHUU3_001673"
     CDS             408..2429
                     /gene="uvrB"
                     /locus_tag="ACHUU3_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR2081766.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(2621..3529)
                     /gene="yvcK"
                     /locus_tag="ACHUU3_001674"
     CDS             complement(2621..3529)
                     /gene="yvcK"
                     /locus_tag="ACHUU3_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR2081767.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      3794..3938
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            3926..4915
                     /gene="moaA"
                     /locus_tag="ACHUU3_001675"
     CDS             3926..4915
                     /gene="moaA"
                     /locus_tag="ACHUU3_001675"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR2081768.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            4937..5449
                     /gene="moaB"
                     /locus_tag="ACHUU3_001676"
     CDS             4937..5449
                     /gene="moaB"
                     /locus_tag="ACHUU3_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR2081769.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            5452..5937
                     /gene="moaC"
                     /locus_tag="ACHUU3_001677"
     CDS             5452..5937
                     /gene="moaC"
                     /locus_tag="ACHUU3_001677"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR2081770.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            5930..6175
                     /gene="moaD"
                     /locus_tag="ACHUU3_001678"
     CDS             5930..6175
                     /gene="moaD"
                     /locus_tag="ACHUU3_001678"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR2081771.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            6177..6629
                     /gene="moaE"
                     /locus_tag="ACHUU3_001679"
     CDS             6177..6629
                     /gene="moaE"
                     /locus_tag="ACHUU3_001679"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR2081772.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            6766..7470
                     /locus_tag="ACHUU3_001680"
     CDS             6766..7470
                     /locus_tag="ACHUU3_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR2081773.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            7741..8388
                     /locus_tag="ACHUU3_001681"
     CDS             7741..8388
                     /locus_tag="ACHUU3_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR2081774.1"
                     /translation="MWLITALGLVATAGLAWGAKYLEITATKYDSPPMYVAIGLLLLC
                     MYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVPVYAIIIVFSTAGAMFLISMLAG
                     LLFNVEPGSHRFIIMMTLTGLALVIIVNAALMSERPIWVISCLMIVLWPGIISHGRNK
                     LLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFGILAAIAITLVWQRHTCFFH"
     gene            complement(8453..9164)
                     /locus_tag="ACHUU3_001682"
                     /pseudo
     CDS             complement(8453..9164)
                     /locus_tag="ACHUU3_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
     gene            complement(9164..10405)
                     /gene="clsB"
                     /locus_tag="ACHUU3_001683"
     CDS             complement(9164..10405)
                     /gene="clsB"
                     /locus_tag="ACHUU3_001683"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR2081775.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(10402..11163)
                     /locus_tag="ACHUU3_001684"
     CDS             complement(10402..11163)
                     /locus_tag="ACHUU3_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR2081776.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            11296..11706
                     /locus_tag="ACHUU3_001685"
     CDS             11296..11706
                     /locus_tag="ACHUU3_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR2081777.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(11668..12774)
                     /locus_tag="ACHUU3_001686"
     CDS             complement(11668..12774)
                     /locus_tag="ACHUU3_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR2081778.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(12785..13927)
                     /locus_tag="ACHUU3_001687"
     CDS             complement(12785..13927)
                     /locus_tag="ACHUU3_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR2081779.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQIWQMQRAEDN
                     GQTFEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEA
                     LLSTEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLL
                     STLGMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPAR
                     YFVSTLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(13920..15656)
                     /locus_tag="ACHUU3_001688"
     CDS             complement(13920..15656)
                     /locus_tag="ACHUU3_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR2081780.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(15649..16644)
                     /gene="hlyD"
                     /locus_tag="ACHUU3_001689"
     CDS             complement(15649..16644)
                     /gene="hlyD"
                     /locus_tag="ACHUU3_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR2081781.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(16647..17318)
                     /gene="cecR"
                     /locus_tag="ACHUU3_001690"
     CDS             complement(16647..17318)
                     /gene="cecR"
                     /locus_tag="ACHUU3_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR2081782.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            17547..18911
                     /gene="rhlE"
                     /locus_tag="ACHUU3_001691"
     CDS             17547..18911
                     /gene="rhlE"
                     /locus_tag="ACHUU3_001691"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR2081783.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(19143..19625)
                     /gene="ybiA"
                     /locus_tag="ACHUU3_001692"
     CDS             complement(19143..19625)
                     /gene="ybiA"
                     /locus_tag="ACHUU3_001692"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR2081784.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            19745..21895
                     /gene="dinG"
                     /locus_tag="ACHUU3_001693"
     CDS             19745..21895
                     /gene="dinG"
                     /locus_tag="ACHUU3_001693"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR2081785.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            21923..22885
                     /gene="ybiB"
                     /locus_tag="ACHUU3_001694"
     CDS             21923..22885
                     /gene="ybiB"
                     /locus_tag="ACHUU3_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR2081786.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVAMGEAATISDGLARVNQAF"
     gene            23026..24111
                     /gene="hcxB"
                     /locus_tag="ACHUU3_001695"
     CDS             23026..24111
                     /gene="hcxB"
                     /locus_tag="ACHUU3_001695"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR2081787.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            24178..24237
                     /gene="ybiE"
                     /locus_tag="ACHUU3_001696"
     CDS             24178..24237
                     /gene="ybiE"
                     /locus_tag="ACHUU3_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR2081788.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(24340..24600)
                     /gene="ybiJ"
                     /locus_tag="ACHUU3_001697"
     CDS             complement(24340..24600)
                     /gene="ybiJ"
                     /locus_tag="ACHUU3_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR2081789.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(24865..25131)
                     /gene="ybiI"
                     /locus_tag="ACHUU3_001698"
     CDS             complement(24865..25131)
                     /gene="ybiI"
                     /locus_tag="ACHUU3_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR2081790.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(25205..25882)
                     /gene="ybiX"
                     /locus_tag="ACHUU3_001699"
     CDS             complement(25205..25882)
                     /gene="ybiX"
                     /locus_tag="ACHUU3_001699"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR2081791.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(25924..27908)
                     /locus_tag="ACHUU3_001700"
                     /pseudo
     CDS             complement(25924..27908)
                     /locus_tag="ACHUU3_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         6557 a         7214 c         7521 g         6616 t
ORIGIN      
        1 cctgtggata accatgtgta ttagagttag aaaacacgag gcaagcgaga gaatacgcgg
       61 cttgcacgcg aattggcgtt aaagacggct caaagaaata tcttttattt tttaactggt
      121 tagataaatg caatggcagt cactgaacag gcatctcttg ccataaaact gtcatcactc
      181 atcttgacaa atgttaaaaa agccgttgct ttggggataa cccggtaagg ccggagtttt
      241 atctcgccac agagtaaatt ttgctcatga ttgacagcgg agtttacgct gtatcagaaa
      301 tattatggtg atgaactgtt tttttatcca gtataatttg ttggcataat taagtacgac
      361 gagtaaaatt acatacctgc ccgcccaact ccttcaggta gcgactcatg agtaaaccgt
      421 tcaaactgaa ttccgctttt aaaccttctg gcgatcagcc agaggcgatt cgacgtctcg
      481 aagaggggct ggaagatggc ctggcgcacc agacgttact tggcgtgact ggctcaggga
      541 aaaccttcac cattgccaat gtcattgctg accttcagcg cccaaccatg gtacttgcgc
      601 ccaacaaaac gctggcggcc cagctgtatg gcgaaatgaa agagttcttc ccggaaaacg
      661 cggtggagta tttcgtctcc tactacgact actatcagcc ggaagcctat gtaccgagtt
      721 ccgacacttt cattgagaaa gatgcctcgg ttaacgaaca tattgagcag atgcgtttat
      781 ccgccaccaa agcgatgctg gagcggcgtg atgtggttgt agtggcctct gtttccgcga
      841 tttatggtct gggcgatcct gatttatatc tcaagatgat gctccatctc acggtcggta
      901 tgattatcga tcagcgcgcg attctgcgcc gactggcgga gctgcaatac gctcgtaatg
      961 atcaagcatt ccagcgtggt actttccgcg ttcgtggcga ggtgatagat atcttcccgg
     1021 cagaatcgga tgacattgca cttcgcgtgg aactgtttga cgaggaagtg gaacgattgt
     1081 cgttatttga cccgctgacc gggcagattg tttccactat tccacgtttt accatctacc
     1141 cgaaaacgca ctacgtcaca ccgcgcgagc gcatcgtaca ggcgatggag gagatcaaag
     1201 aagaactggc cgccagacgc aaagtgctgt tggaaaacaa caaactgctg gaagagcagc
     1261 ggctgaccca gcgtacccag tttgatctgg agatgatgaa cgagctgggc tactgttcgg
     1321 ggattgaaaa ctactcgcgc ttcctctccg gtcgtggacc gggtgagcca ccgccgacgc
     1381 tgtttgatta cctgcctgcc gatgggctgc tggtggtcga tgaatctcac gtcaccattc
     1441 cacaaattgg cggcatgtat cgcggtgacc gggcgcgtaa agagacactg gtggagtacg
     1501 gcttccgcct gccatcagcg ctggataacc gtccgcttaa gtttgaagag ttcgaagcat
     1561 tagcgccgca aaccatctat gtttcggcga cgccgggtaa ttacgagctg gaaaaatccg
     1621 gcggcgatgt ggtggatcag gtggtgcgtc caaccggatt gcttgacccg attatcgaag
     1681 tgcggccggt ggcgacacag gttgatgatc ttctttcgga gattcgtcag cgagcggcaa
     1741 ttaacgaacg cgtactggtc accacactga ccaagcggat ggcggaagat cttaccgaat
     1801 atctcgaaga acatggcgag cgcgtgcgtt atcttcattc agatatcgac accgtcgaac
     1861 gtatggagat tatccgcgac ttgcgtctgg gtgagttcga cgtgctggta gggatcaact
     1921 tactgcgcga aggtctggat atgccggaag tgtcgctggt ggcgatcctc gacgctgaca
     1981 aagaaggctt cctgcgttcc gaacgttcgt tgatccagac tattggtcgt gcggcacgta
     2041 acgttaacgg taaagcgatt ctctacggcg ataagatcac cccatcaatg gcgaaagcga
     2101 ttggcgaaac cgaacgtcgc cgtgagaaac agcagaagta caacgaggaa cacggaatta
     2161 cgccgcaagg cttgaacaag aaagtggtcg atatcctggc gctggggcag aacattgcca
     2221 aaaccaaagc gaagggcaga ggaaaatcgc gcccgattgt tgagccggat aatgtgccga
     2281 tggatatgtc gcctaaagcg ttgcaacaga aaatccatga gctggaaggg ttaatgatgc
     2341 aacacgcgca gaatctggag ttcgaagaag cggcacaaat tcgtgaccag ttgcatcagc
     2401 tgcgtgagct gtttatcgcg gcttcgtgac aggataacga agaagacaga tgacaaaagc
     2461 aaaattgcct gatgtgctac gcttatcagg cctacatgat ctctgcaata cattgaatta
     2521 gcgcggtttg taggccggat aaggcgttca cgccgcatcc ggcataaaca acgcgcactt
     2581 tgtcttcaat aggaagccgg aatttttctt cggatttccg ttaacctaaa gcctgtaacg
     2641 ccttttccag cgcgttatgt aacaactggc ggtcatgacg atacggaata tcgctggcct
     2701 ccagtacctc ctgaatcaca atccgctctt tcaccgccga gacatccact tttggcccga
     2761 cgatgaccgc atcaatgacc tttttaccaa catactgctc cataattgcc agcttgcttt
     2821 ccagcttcaa attagccgca ggtaaactca actcacgccc cagattgccg atatagacca
     2881 tcggcgctgg cgtgcggcgt aatgcctggg cgatttcctt cagcagcaga attggcatca
     2941 ggctggtata aaaactgcca ggcccaataa tgatgagatc cgcttcattg atagcgtgaa
     3001 ccgcctcacg cgttgcgggt acattcggcg ttaataataa ctcttgaatc ggcgtagtta
     3061 actggtcgat attgacctcg ccgtaaactt catgcccctg atcgtcaatc gccatcagat
     3121 cgacgggatg ctctgacatt ggaatcaaat gcgtatccac tttcagcaga ttacgaatta
     3181 aattgatggc ttccagaggc cgcacgctaa ggtgatccag cgcctttaac atcaagtttc
     3241 cgagattatg accggaaagt tcgccattgc cgccaaaacg gtattcaaac atcgcggagg
     3301 cgacgctcgg ttccgttatc agttggttga ggcagttgcg catatcgccc caggcaatgc
     3361 cgccttctga acggcgaata cgccccgtcg agccaccatt atccgtggtg gtgacgatac
     3421 ccgttaaacg agaacccaaa gacgaaagtg atgagagaac gcgtcccagt ccatgccctc
     3481 cgccgagagc aacgacacga tcaagatcag ccagcgtacg attgcgcata tagtttcctg
     3541 gagtcagatt atccgcgcta cagtagcgca aattgcgggg aaacagcaat taacctgcca
     3601 atattgagga tacaaactat tttcttctac ctctaaagga cgatgcacgc tatgcctccc
     3661 tgatgatgta tatcaaagta aaactgccat tttcccttat tctgtagcga aatagcacga
     3721 tcatgacgct atatacatga ttacatagcg aaagtgtgga tggtaaaaat ctcatttaca
     3781 cgctagtatc ggcttaacca ctaaacactc tagcctctgc acctgggtca actgatacgg
     3841 tgctttggcc gtgacaatgc tcgtaaagat tgccaccagg gcgaaggaag aaatgacttc
     3901 gcctcccgta tctggaaagg tgtacatggc ttcacaactg actgatgcat ttgcgcgtaa
     3961 gttttactac ttgcgcctgt cgattaccga tgtgtgtaac tttcgttgca cctactgcct
     4021 gccggatggc tacaaaccga gcggcgtcac caataaaggc tttcttaccg tcgatgaaat
     4081 tcgccgggtt acgcgcgcct tcgccagtct gggcaccgaa aaagtgcgcc tgacaggagg
     4141 agagccgtct ttacgccgcg actttaccga tatcatcgcc gctgtgcggg aaaacgacgc
     4201 tatccgccag attgcggtca ccaccaatgg ttaccgtctg gaacgcgatg tggcgaactg
     4261 gcgcgatgcg ggacttactg gcattaacgt cagtgtcgac agtctggacg cccgccagtt
     4321 tcacgctatt accgggcagg ataaattcaa ccaggtcatg gcagggatcg atgctgcatt
     4381 tgaggtcggt tttgagaagg tcaaagtcaa taccgtgctg atgcgtgatg ttaatcatca
     4441 ccagctcgac acctttctga actggatcca gcatcgccct atccagctgc gtttcatcga
     4501 actgatggaa acgggcgagg gcagtgagct cttccgtaag catcacatct ctggtcaggt
     4561 tctgcgtgac gagctactgc gtcgcggctg gatccaccaa ttacgtcaac gcagcgacgg
     4621 tcccgcgcaa gtcttttgcc atccagatta cgccggagag attggcctta tcatgccgta
     4681 tgaaaaagac ttctgcgcca cttgcaaccg cctgcgcgtt tcctccattg gtaaactcca
     4741 tctctgcctg tttggtgaag gcggcgttaa cctgcgcgat ctgctggaag acgataccca
     4801 gcaacaggcg ctggaagcgc gtatttcagc ggcgctgcgg gagaagaaac agacccattt
     4861 cctgcatcaa aacaacaccg gtattacgca aaacttatcg tacattggcg gctaaaacgt
     4921 caaaaggaga gatcagatga gtcaggtaag cactgaattt atcccgaccc gtattgctat
     4981 tcttacggtt tctaatcgtc gcggtgaaga agacgatacc tccggtcact atttgcgcga
     5041 ttcggcgcaa gaagcgggcc atcacgttgt cgataaagcc attgtgaaag aaaaccgcta
     5101 cgctattcgc gctcaggtat ctgcgtggat cgccagcgac gatgtacaag tggtgttgat
     5161 tacggggggg actggcctga cggaaggtga tcaggctccc gaagcattgc tgccgttgtt
     5221 cgaccgtgaa gttgaaggtt ttggtgaagt gttccggatg ttgtcgtttg aagagattgg
     5281 cacttccacg ttgcaatctc gtgcggtagc gggcgtcgcc aacaaaacgc tgattttcgc
     5341 catgccgggt tcgaccaaag cgtgccgtac cgcatgggaa aatatcatcg cgccgcagct
     5401 ggatgcccgt acgcgtccgt gtaatttcca tccacatttg aagaaataag tatgtcgcaa
     5461 ctgacccata tcaacgccgc tggcgaagcg cacatggtgg atgtctccgc caaagcggaa
     5521 accgtgcgtg aagcgcgggc ggaagccttt gtcaccatgc gcagcgagac gctggcgatg
     5581 attattgatg gtcgccacca caaaggcgac gtatttgcca ctgcgcgtat tgtcggtatt
     5641 caggcggcaa aacgcacctg ggatctgatc ccgctgtgtc acccgctgat gctcagcaaa
     5701 gttgaagtca atttacaggc cgagccggag cacaatcggg tgcgtataga aaccttatgc
     5761 cgcctgaccg ggaaaaccgg tgtcgaaatg gaagcgttaa ccgcggcctc cgtggcggcg
     5821 ctgaccattt atgacatgtg caaagcggtg caaaaagata tggtgattgg tccggtacgt
     5881 ttgctggcga agagcggcgg caagtcgggt gactttaagg tggaagcgga tgattaaagt
     5941 tctttttttc gcccaggtgc gcgagttggt gggaacagat gcaaccgaag tggctgcgga
     6001 ttacccaacc gttgaagcgt tacgccagca cctggctgcg cagagcgatc gctgggcgct
     6061 ggcgctggaa gatggcaaat tgctggctgc cgtcaaccag acgctggtga gttttgacca
     6121 tccgctgact gacggcgacg aagtagcttt cttcccgccg gtaaccggag gttaagatgg
     6181 cagaaaccaa aattgttgtc ggcccgcagc cgttcagcgt aggagaagag tacccgtggc
     6241 tggctgagcg tgacgaagac ggtgcagtgg tcacgtttac cggtaaggtg cgcaaccata
     6301 acctgggtga cagcgtcaaa gcattaaccc tcgaacacta tccggggatg actgaaaaag
     6361 cactggcaga aatcgttgat gaagcgcgta accgctggcc gctggggcgc gtcacggtga
     6421 ttcaccgcat cggggaatta tggccgggcg atgaaatcgt ttttgtcggt gtcaccagtg
     6481 cgcatcgcag cagtgcgttt gaagccgggc agtttattat ggattatctc aaaacccgcg
     6541 caccgttctg gaagcgcgaa gccacgccgg aaggcgaccg ctgggttgaa gcccgggaga
     6601 gcgatcagca ggcggcaaaa cgctggtagt tttttgttag ccggataagg caccgctgcg
     6661 tccggcaatt tcaccgctca gtacatgcgt ttacgctgcg tcgatgcaca gcctcatcat
     6721 tttgcagtat ccttaagata ttccttatat cttcaggaga tcgtcatgga cagattccca
     6781 cgttctgatt caatcgtaca accccgggct ggcttgcaaa cttatatggc gcaagtctat
     6841 ggctggatga ccgttggctt gttgctgacc gcatttgttg cctggtatgc ggctaattcc
     6901 gcggccgtga tggagctgtt gttcactaac cgtgtctttt taatcggtct gatcatcgcg
     6961 caattagcat tggttattgt gttatcagcg atgattcaaa agctgagcgc aggtgtaacg
     7021 acgatgctct ttatgcttta ttcggcgctg acgggtctta cgctttccag tatattcatt
     7081 gtctataccg ctgcttctat cgccagtact ttcgtcgtta ctgccgggat gttcggcgca
     7141 atgagcctgt acggttacac cacgaagcgc gatttaagtg gctttggcaa tatgctgttt
     7201 atggcgttaa tcggcattgt gctggcatcg ctggtcaact tctggctgaa aagcgaagca
     7261 ttgatgtggg cagttaccta catcggcgtg attgtctttg tcggattgac ggcgtatgac
     7321 actcagaaat tgaaaaatat gggtgagcag attgataccc gcgacacgtc gaacctgcgc
     7381 aaatattcca ttcttggcgc gttaaccttg tatctggact tcatcaacct gttcctgatg
     7441 ttgttacgga tcttcggcaa ccgccgttaa ttctttttca ccatcactat cccggccatg
     7501 tgtcgggatt tttttcttac taaaaacgac acttaacaag taccaggtat aaaatatgcc
     7561 ttatgagcga gcggaatcgt tttccgtatc cataaataca aaagtatttt gtatgcgtgg
     7621 ttttaaattt attgatggtg aatattaata ttttttctaa ggacaactaa aagaatggaa
     7681 tcatactcgc aaaacagcaa taaattagat tttcagcacg aggccaggat ataaaacggt
     7741 atatggctca ttaccgcttt aggtttggtg gcaaccgcag gactagcctg gggagctaag
     7801 tatctcgaaa ttacggcaac caaatatgat tcaccaccaa tgtatgtcgc tatagggtta
     7861 ttattacttt gtatgtatgg cttaagtaag gatatcaaca agataaatgc cgccatcgcg
     7921 ggcgtaatat atctgttttt actctctttg gtggcgattg tcgttgcaag tttagttcct
     7981 gtatatgcca ttatcatcgt gttcagcact gcgggcgcga tgtttttaat cagtatgctg
     8041 gccggtttat tatttaatgt tgagcctggt tctcaccgtt ttatcattat gatgacgttg
     8101 acagggttgg ccctggtaat catcgtgaat gcggcattaa tgagtgaacg gcccatttgg
     8161 gtaataagtt gcttaatgat tgtgttatgg ccaggcatta tctcgcatgg acgaaataag
     8221 ctccttgaac tggcaggaaa atgccatagt gaagagttgt ggagtccggt tcgttgcgct
     8281 tttacaggtg cattaacact ctattactat tttatcggct tctttgggat acttgccgcg
     8341 atagctataa cgcttgtctg gcaaaggcat acgtgttttt ttcattagcc gtaaatgcac
     8401 atactaaaga acttaactaa acttcacatc gccgcttcat ttttcgcccg tagcccagta
     8461 caaagaaata gttcacgctt tgaccaagta acagccagat aatcgccccc attaccatcc
     8521 agttaacgct ggaaatcagc atctgagcga gggcgaattt ccatgaaggc agcaccagct
     8581 tttgcccttt gatggtcata tggcggtgct tcgcgaaagc gcaaaaccac aaataaaccg
     8641 cgataatcat cagtaagcca atgccgagaa tacgcagcgt agtttgatcg acataccagt
     8701 gatccggcaa ctccaccacg cctgcggtaa agataatccc tgccagtaaa atatagccca
     8761 gccagttggt ggtaatactg agcgagaaaa tccgcgtaat agtgctaccg ggcaacccca
     8821 gacgagagta caaacgatag cgcataccaa tgccgccgac ccaggtactg agcgtcaggt
     8881 tgaaggcgta gcagataaac gacaccagca tcacctggcg cttcgccagt ttgtgaccgc
     8941 agtaaaagcg ggcgagcagg tcatagcagc cgtaaatcag atagctgacg accaccagcg
     9001 accgcactaa gcagcgcaac gcgattgtag tcgcggatga ccttccagac ctcttcccag
     9061 tccacttttt tggcgtagac caccagcaac acaatcaccg cgataaaaaa cagccaggtg
     9121 aggatcttct ttgctaagcg ccagcgcggg tgtgatttac tcatcagggt tttacccccg
     9181 tgttttcagt ttctacccga tcctgcgttt ccattgtcgg ttgtgcgggc ggatcaacct
     9241 gcgccagacg tggcgtgtgt gccggaagcc agccaaccag cgccgggaag tggcgtaaaa
     9301 agtggaacgc cagcacgctt ttggtcaggt tccaccaggt gcgtttgggc agcatggttt
     9361 catccacctg ctgacaatct gcggcgataa tgccgttcag attatcgcgc agcgtctggt
     9421 taaaattacg atcgtggatg atgacatttg cttcgagatt cagtgacaaa ctgagcggat
     9481 cgagattact ggaccctact gtcgcccagt gatcgtccat caatgctact ttgccgtgga
     9541 gcgggcggcg gcggtactca aacacctgaa cgccgccttt aaccagatag ttatacagca
     9601 agcgcgcgcc gactctgaca atcggcatat ccggttcgcc ctgaatgatc agtttgatcc
     9661 gcaccccgcg gcgtgccgct ttacgcaagg cgtgtaaaaa tcgatagccg gggaagaagt
     9721 aggcgttggc gataatgact tcccgccgcg cctgagtgag cattttcaga tagtggcgtt
     9781 ctatatcatc gcggtgttct tcgttatcgc gccagaccag caatacctgc gcgtctcccg
     9841 gctggcggtt ctcttccgct ttgtgatgac gtcgccacca gcgtcgtgcc gcgctctgtc
     9901 caggcaggtt ttccagctca aactggagaa tatcttcgac aatcggccct tcaaggcgta
     9961 ccgcgtaatc ctgtttagcc tctggaccgt agctggacat atgctcggcg gagtaattca
    10021 gcccgccaat aaaggcgata cgcgcgtcga tcaccacaat tttgcgatgc atccggcgaa
    10081 acacattggt gcgcatacca aaaaggcgag ggcggggatc gtagtagcgg aacactacgc
    10141 cagctgccgt cagttcattg acaaactcat cgctgagatc cggcgaaccg tagccatcca
    10201 gcaagacttc cgctttaacc ccgcgttgcg ctgctgccag tagtgccgca tgcagctgtt
    10261 tgccgacgtt atcctcaaac cagataaacg tttcaagaat gatgcgttct tgtgcctcgc
    10321 caatcgcctt aaacaccgcg ggataatatt gctcgccgtt ttccagcaac tggatcttat
    10381 tgccttcgcg ccagctacat ttcataaatg aatctccgca cttaaagggg catgatcaga
    10441 aaggtgtcgc catgtccgca gcggcaacgc ggttggcgcg ctggcgctgg catttttgac
    10501 gtagatcctg tccagtcgta gtagaggaaa ttgcaccgga aacgtgcgcg ccgggcgtcc
    10561 gtgggcgcgg gtaaaaatct catccagtcc ggcctgcact tttaacggat gattagcttt
    10621 ttgccgccag tcattgaaat cacccgccac caataccggt tcgccgtccg gtagctcatt
    10681 cacccattcg gcgagcatcg caagctgcgc ctgacggtgc gcctcacgca ggcccagatg
    10741 tacgcacatc acatgaatcg cttttccggt catcggcggc acaatgcggc agtagagcac
    10801 gccgcgcttt tccgcaccat cgaccgaaac atcgcgattc tcataatgtt caatgggata
    10861 acgcgacagt acggcgttgc cgtgatgccc ttccgggtat acggcattgc gaccgtaggc
    10921 aaaatcgctc cacatagtgt cggcgagaaa ctcgtagtgc gaggtatcgg gccagttttc
    10981 cacatgcagc ggatgaactt cgtgcgcgcc catcacttcc tgcaggcaaa caatatcggc
    11041 gctgacggta cgcacggcat cgcgaagttc cggcaaaatg aagcgtcggt taaacgcggt
    11101 aaagcctttg tgaatgttga tggtgagcac cttgaacgaa aattgttgtg tttgatcggg
    11161 cataattttc ctgtctttgc ctcttatctc attgaaatag tgtagtcggc gtcacaaaaa
    11221 ggtgcggtct tacggaattt tccgtaaagt tcggtactct gagtaagtag agataaattc
    11281 ttcaggagag aagccatgaa gtggcaacaa cgtgttcgtg tcgcaacggg tctaagttgc
    11341 tggcagatta tgttgcattt actggtagtg gcgctgctgg tggtgggctg gatgagtaag
    11401 actctggttc acgtcggcgt gggattatgc gcactgtatt gtgtcacggt agtgatgatg
    11461 ctggtgtttc agcgccaccc cgagcaacgc tggcgtgagg tggcagacgt gctggaagag
    11521 ctgaccacga cctggtattt tggcgcagcg ctgattgtgc tgtggctgtt gtcccgcgta
    11581 ctggaaaaca actttttgct ggcaattgca gggctggcga tccttgctgg cccggcggta
    11641 gtgtctttgc tggcgaaaga taagaagtta catcacctta cgtctaaaca tcgcgtacgc
    11701 cgctgaccct gtcgtggccg ttatcaccag tagcggccac aaactattcc acacaatatc
    11761 cagactcgca tccttcaaat aaatctgctt ggtaatgtcc gtaaagtggc gaatagggtt
    11821 aatccacgtc aggttttgca gccatactgg catgttttcc accggagaaa cgtaaccgga
    11881 aaggagaatg gcgggcatca taaaaacaaa cacgccgata aacgcctgct gttgtgtcga
    11941 gcagagtgat gaaatcaaca gaccgaatcc caccagcgat aaaccgtaaa tcaccatcgt
    12001 aaagtagaac agcgccagcg atccggcgaa ggggatttga tacgcccaga taccaatcgc
    12061 cagcacaatg gtggcctgga aggtggcgac aattaacgcc ggtacggctt tgccgatgaa
    12121 gatctgccag gtggtgagcg gcgaaaccag tagctgatcg agcgtacctt gttcacgttc
    12181 gcgggcgacg gaaagtgaag tgacgatcat tacgccgata gtggtgatca tggcgatcag
    12241 cgacggcacc acaaaccatt tgtagtcaag attcgggtta taccagttgc gtaccaccag
    12301 ctcgctgttg ttaggtttcg gttttccttc cagcagctcc tgctgataat ttttgacgat
    12361 ctgttgcagg tagttggcgg caatttgcgc actgttggag ttacgcccgt cgaggatcaa
    12421 ctgcaacggc gcggtctgga aggtatccag tttgcgcgag aagtcagccg ggaaacgcac
    12481 cagtagtaac gccttttgtg tgtcgatggt tgggcggatc tcctgtgggc ttttcagcag
    12541 cagcacatgg gtaaaggcgc tggcgcgggc aaaacgttgg gtcagctcca ccgaatgctc
    12601 gccgttatct tcatcgtaga tggcgatggt ggcgttagtc acttccagcg tggcggcgaa
    12661 cgggaacagg atcacctgaa taagcacagg taaaatcaga atcgcgcggg tttgtggttc
    12721 gcgcagcaac gactgcaact ctttgcggat taacgtccat aagcgatgaa acatgctctt
    12781 ctccctaatc cagccgacgt ttggttttca gccacgtcag gccgataaac atcaccgccg
    12841 aagcgatcaa aaacagcacg tttaccacca gcaccactgg aatattcccg gcgaggaaca
    12901 ggctttgcag ggtgctgacg aaataacgag cgggaataat gtacgtcacc gcgcggatca
    12961 ccgcgggcat actgtcgatc tgaaaaataa agccggaaag cataatcgac ggcaggaacg
    13021 cggcgttcag ggcgacctga gcggcattga actggttgcg ggtaatcgtg gaaatcagca
    13081 gccccatccc cagggtactg agtaaaaaca ggctggagat aaaaaacaga ataagcagcg
    13141 aaccgcgata cggcacgccg agaataaaca ctgacaccag catacacagc agcatcgcca
    13201 gcatcccgag aaagtaataa gggatcagct tacacagcag cagttccgtg cgggtaatct
    13261 ccgtagagag cagagcctcc atggtgccgc gttcccattc tcgcgccacc accagcgagg
    13321 tgagaatcgc gccgatgacc gtcatgatga tggtcactgc accggggata atgaagtgct
    13381 ggctaatcgc cgccgggtta aaccagtagc gggtttgtac atcaataagc ggttcaaaag
    13441 tctgcccgtt gtcctccgct cgctgcattt gccagatctg ccagatctgc cagatccctt
    13501 cgacataccc ctgtacaaag ttagcggtat tcggctcact gccgtcggtg atcacctgaa
    13561 tcggtgcggt ggcgttggcg cgctccatct gttccgcaaa atccaccgga ataaccacca
    13621 gaccgcgaat tttccccgcc tgcattttgg cgatcagttc ctgacggtta tcgctgatgg
    13681 tggcgtcgat gtagggcgaa ccggtcatgg tgtgggtgaa atccagcgcc gcttcgctac
    13741 gctgttccag caagatcccg acccgcagct tgctggagtc gaggttaatg ccataaccaa
    13801 aaataaacag cagtagcagt gggatcacta ccgcaatcag ccagctactc ggatcgcgaa
    13861 cgatctgccg cgtctctttg acgcacagcg cccgcacgcg acgccaggaa aggatcgggt
    13921 tactcattgc tatgctcctt atcccagtcg tggatcaact gaataaaggc ttgctccatg
    13981 gtgggatcgg gttgctcatc gttagccgac tgtgctttca aatcgtccgg cgtaccgctg
    14041 gcgattaatt tcccacggta caccaggccg atgcggtcgc aatattccgc ttcatccata
    14101 aagtgggtgg tgaccatcac cgtgacgcct ttctctacca tgctgttgat atgtagccaa
    14161 aattcacggc gggtgagggg atcaacgccg gacgtcggtt cgtcgagaaa cagaatgtcc
    14221 ggttcatgca tcagcgaaca ggccagcgcc agccgctgtt taaaacctaa tggcagttca
    14281 tcggtggcgt gggaggcgat acttttcagg ccgaacgcct cgctcatgcg ggagattttt
    14341 tcgttctgcg cccgaccgcg taagccatac acaccagaga aaaagcgtaa attctgttcg
    14401 accgtcaggt taccgtagag cgaaaacttt tgcgccatat agccgagatg ctggcgcgct
    14461 ttaccagaac tctctttcag atccatcccc agtaccagcg cctgaccaga ggtcggcacc
    14521 agcaaaccgc acatcatctt aaaggtggtc gatttgcccg cgccgtttgg ccccagcaaa
    14581 ccaaaaatct ccccgcgttt aacggcaaag ttgacgtgat cggtggcggc aaaatcacca
    14641 aatttcttgg tcagttcttt cgcttcgatc accgtctcgc cgggtgtgcc ttctaccgta
    14701 tgtaatattg cgcccagcgg cgattccgag gttccggcac cgcccagcaa atcaataaac
    14761 gcatcttcaa aacgcggcgt agtttcgttg atgttgattt ccggcatccc gtcggcatgg
    14821 cgaatatcgt ctggtgtggt ctcttttttg aggatcagac gtaccgattt cccctgaatc
    14881 atgccgtcgc tgacctgcgg cagtttcaat gcccgttgca acagtttgcg gttgccctcg
    14941 tgtggacttg tcatcagaaa gctgcgtccg gccatggttt gtgtcagggc ttttggttcc
    15001 ccctgataca gcaactcgcc ttcattcatc agtaacacgt cacggcactg ctcggcttcg
    15061 tcgagatacg aggtactcca gaggattaac atcccttcgc ctgccagctc atgcaccatc
    15121 tgccacagtt cgcgccgtga gatagggtca acgccgacgc cgggttcatc gagcagcaac
    15181 actttcggtt cgcccaccag ggtacaggcc aggccgagtt tttgtttcat cccaccggag
    15241 agcttgcccg ccaggcgtcc ggtaaacggc ccaagagacg taaactccag caggcgagca
    15301 aaagtttgct tacgtgcctc gccggtgacg ctgcgcaaat ccgcgtacag attgaggttc
    15361 tccatcaccg tgagatcttc atacagacca aatttctgcg gcatatagcc gagcacggcg
    15421 tgcagcgcgc cgtcgttttt gattggatca aagccaatca ccgtggcatt gccgctgtcg
    15481 ggtttcagta atcccgccag catccgcatt agcgtggttt tacctgcacc atccggcccc
    15541 accagccccg tcacataacc ggcgtgaatg gtacaatcga gcggcgcgac ggcgggcttg
    15601 tccatgcccg gaaagcgttt ttccaggccg ttcagcgtga taacggcatc attcatgtcc
    15661 tgcctcgtta ccgaattgca ccgtcaccgg cattccctgg cgtaacgcat catcggcgtc
    15721 ggtcaccaca atacgcaggc gatagacgag gtcggtacgc agatccggtg tttcgacggt
    15781 tttcggggta aattcagcag tcggcgaaac gaaaccaatc tgcccgtgat acggcttgtc
    15841 cgggcgacca tcggtataaa gcagcacttt gcgccccggc tgggcctggt caagataccg
    15901 ttcatcaaca taagcgcgca cccacaccgg acgcgttagt gaaacggtaa acaccgtgcc
    15961 accttcattg aggaccgtgc ctggctccac cgcgcgcgtt aacagcgtgc catcagacgg
    16021 ggctatcaac gttgagtcct gtaaattcaa ctccgcctgc gccagttgcg cctgcgcctg
    16081 ttcgaggctg gctttcgcct gggcgatgtc ctgttcacgg ttaccggaac ggtactgacg
    16141 caatttatcc tgtgctgatt tcagcgttgc ctgcgcctgg tcgcgcgagg agcgggcatt
    16201 ttccaggtca tttgccgaaa tagtgcggct tttccacagt ccttgctggc ggttatagaa
    16261 gttctgcgca tagtcatagg cggcttgcgc ctgtttcacc gctgcggcgg cctgagcgat
    16321 ttcttcatcg cgatacccgg caagcatcag atcatactgc gcctgtgcca ccgagacgcc
    16381 cgctttcgcc tgcatcaggg caatctcata cggcttgtga tccagttcgc ccagcacctg
    16441 gcccgctttg atagcatcac cttcgtccac cgccagcgat tcaacgcgcc ccccaacacg
    16501 gaaactaaga ttcaccgtac gaatatccac gttgccatac agcgtcaggc cgttatcctg
    16561 gcggctttga taccaccagt agcctccggc aaccacggcg gcaagtacca ctaccgccag
    16621 ttcgatcacg acaggttttt tcatcactac aaactccttt gcgataatcc ttgcaaaatc
    16681 aggtcgatat gacaggtcac cgtctggttg atcagttcgg tcttttcttc atcgaacgcg
    16741 gtccagccgg tacgtaacag aattgtttct ttgccaagac ggaacgccag aatctcacca
    16801 atcaacgcat gggtatgaag gatcatgcgg gtgtcattgg cgtcgctgcc ggtccaggcg
    16861 gcaatcaggc gtgtcaggtg gctgtgtagc ggactaatca cctgttcgtg caccaggtgg
    16921 taggctgccg tgggagagag ctgctcacgg gagataaact tgctgaggtt gacggtgtca
    16981 tcctgggtga gcagcttaat catgttcctg caggcgcgaa ggagcagttc acggatggca
    17041 gcccgatcag gctgtggttg ggcgaacaag cgttcggctt cctcggcatg cggacggaac
    17101 tgctcgccaa taaaatcggc aatccactgg gcgcaggcga ggtacaaatc ttctttcgaa
    17161 ccgaagtagt aggtgatggc agcgatattc tgcccggctt gggcggctat ctcgcgggtg
    17221 gtggcgttca ttccatattc accaaactgc gccagtgcgg cagcaatcag ctgttttttc
    17281 gcctgttcac ccttgattgt catggcagga ttattcatcg cacagtccat tcttaatcaa
    17341 atgattgatt aagattatga ctccatatgg gagttgtcca gtatggctaa gaattttagc
    17401 tacgccagtc acagggataa tttatgcgct gcgtcacaaa aactgctaca ctccgctcgc
    17461 tcatgacatt gtggtttttg tcattttcct tttcagtatc tccctgaaaa ctacaccggt
    17521 aacggtcggg gcggttcgga gtagtcatgt ctttcgatta tttgggttta agccctgata
    17581 tcctgcgcgc cgttgccgag cagggttacc gtgaacccac ccctattcag cagcaggcga
    17641 tccctgcggt gctggaaggc cgcgacctga tggctagcgc ccagaccggc accggcaaaa
    17701 cagcgggctt tacgctgccg ctgttgcaac acctgatcac tcgccagccg cacgccaaag
    17761 ggcgtcgtcc ggtacgtgcg ctcattctta ccccgacccg tgaactggcg gcgcagattg
    17821 gcgaaaacgt ccgtgattac agcaaatacc tgaacattcg ttcgctggtg gtgtttggtg
    17881 gtgtgagtat taacccgcag atgatgaaac tgcgtggcgg cgttgatgtg ctggtggcaa
    17941 cgccgggacg tttgctggac ctggaacatc agaatgcagt gaagctggat caggttgaaa
    18001 tcctcgtcct cgatgaagct gaccgcatgc tcgacatggg ctttatccac gatatccgtc
    18061 gcgtgttaac aaaactacct gcgaagcgcc agaacctgtt attctccgcg accttctctg
    18121 acgatattaa agccctggcg gaaaaactgt tgcacaaccc gctggaaatc gaagtggcac
    18181 gccgcaatac cgcgtctgat caggtgaccc agcacgttca ctttgtcgat aagaaacgca
    18241 aacgcgaatt gctgtcgcac atgattggga aagggaactg gcagcaggtg ctggtgttta
    18301 cccgtaccaa acacggcgct aaccatctgg ctgaacagct caataaagat ggcatccgta
    18361 gtgcggcgat ccacggcaat aaatcgcaag gtgcgcgtac tcgtgcgctg gctgatttta
    18421 aatcgggcga tattcgtgta ctggtggcaa ctgacatcgc tgcgcgcggc ctggatattg
    18481 aagagctgcc gcacgtcgtc aactatgaac tgccaaacgt accggaagat tatgtccatc
    18541 gtatcgggcg taccggtcgt gcggctgcta ccggtgaagc gttgtcgctg gtgtgtgttg
    18601 atgaacacaa actgctgcgt gatatcgaaa aactgctgaa aaaagagatc ccgcgcattg
    18661 cgattccggg ctatgagccg gacccgtcaa tcaaagccga accgatccag aacggtcgcc
    18721 agcaacgtgg cggcggcggt cgtgggcaag gtggtggtcg cggtcaacag caaccacgcc
    18781 gtggggaagg tggcgcaaaa tctgcaagcg cgaaacctgc agaaaaaccg tctcgccgcc
    18841 tcggcgatgc caaaccggca ggcgaacaac aacgtcgccg ccgtccgcgt aaacctgccg
    18901 ctgcgcagta atcttttatg ccgggctatg cccggcatca ggctgatgaa caaacgcaaa
    18961 actgcctgat gcgctacgct tatcaggcct acgtgaactc tgcaatatat tgaatttgca
    19021 tgcttttgta ggccggataa ggcgtttacg ccgcatccgg catttcacaa caagcacttg
    19081 tcagcaattt gagaacacgg gaaaaaattt atctgtttta ccgcccataa gccaccgaga
    19141 cgttacttct ctatagccaa ttgttctcgc aactccatta aaaggtagcc cagtcgattc
    19201 ttgcccttac cattaccacc gtctccccag taagcatcgt tttccgtatg ctcaaccagt
    19261 tttgcgggcg cggttgccag caagagcaca cgcagttctg catgctgttc gaatttagca
    19321 cgaagtgctt tacgcatcac ctgttcttta accgactccc agtttttacg aagtggcttg
    19381 gaacgattac gccccatgcg agcggcgacc atgggggaag aaacccgacg gatctcttcg
    19441 cggtattttt catcaaggaa tttttgtgcc tgaaaatagt gttcggaggt gggccaggtt
    19501 ttcccgtcaa ccttgatggg ccaggcggca aagttagaaa aatcaccgta gtcatcgctg
    19561 gtgctgtaga aatttatgat ggtgtcttgc atgacgtgtt ggatcctttg tgctcgaacg
    19621 ggcattaaac cgcattatgt tggtggttat tgcgagccgc tttccagaaa cagaaaaacc
    19681 attacccctg aaaaccgaaa aatgccacaa tattggctgt ttatacagta tttcaggttt
    19741 tctcatggca ttaaccgccg cgcttaaagc gcaaattgcc gcctggtata aggcgcttca
    19801 ggaacagatc cccgacttta ttccccgtgt gccgcagcgg cagatgattg cggacgtcgc
    19861 caaaacgctg gccggagaag aagggcggca tctggcgatt gaagccccca ccggcgttgg
    19921 gaaaacgctc tcctatttga tccccggcat cgccattgcc cgcgaagagc aaaaaacgct
    19981 ggtggtgagt accgccaacg tggcgttgca ggatcagatt tacagcaaag atttaccgct
    20041 gctgaaaaag atcattcccg atcttaaatt cactgccgct ttcgggcgtg ggcgctacgt
    20101 ttgtccgcgt aatctgacgg cgctcgccag cactgaaccc acgcaacagg atctgctggc
    20161 gtttcttgac gacgaactga cggcaaataa tcaggaagag caaaaacgtt gtgcgaagct
    20221 gaagggcgat ctcgacactt atagatggga tggtctgcgc gatcatacgg atatcgctat
    20281 agatgatgat ctctggcgtc gtttgagtac cgacaaagcc agctgcctca accgtaactg
    20341 ttactactat cgtgaatgcc cgttttttgt tgctcgtcgg gagattcagg aagcggaagt
    20401 ggtggtggca aaccatgcgc tggtgatggc ggcgatggaa agcgaagccg tactgcctga
    20461 cccgaaaaat ctgctgctgg tgctggacga aggtcatcac ctgccagatg tggcccggga
    20521 tgcgctggag atgagcgctg aaatcaccgc gccctggtat cgactacagc tggacttgtt
    20581 cacgaaactg gtcgctacct gcatggagca gtttcgcccg aagaccatcc caccgctggc
    20641 gatccctgaa cgtttgaatg cgcattgtga agagttgtat gagcttatcg cctcgttaaa
    20701 caacattctc aatctctaca tgcctgccgg gcaggaggca gagcaccgtt ttgcgatggg
    20761 cgaactgcct gatgaagtgc tggagatctg ccagcggctg gcaaaactca ccgagatgct
    20821 gcgtggtctg gcggagttat ttcttaacga tttaagtgag aaaaccggca gccatgacat
    20881 tgtacgcctg catcggttga ttttgcagat gaaccgcgcg ttggggatgt tcgaggcgca
    20941 aagcaaactc tggcggctgg cgtctctggc gcaatcttcc ggtgcgccgg tgaccaaatg
    21001 ggcgacgcgg gaagagcgcg aagggcaact gcatctctgg tttcactgcg tggggatacg
    21061 tgtcagcgat cagctggaaa ggctgctgtg gcgcagtatt ccgcacatta ttgtcacctc
    21121 cgcaaccttg cgttcgctga atagtttttc gcgtttgcag gagatgagtg gtctgaaaga
    21181 gaaagcgggc gaccgttttg tggcgctgga ttcccccttt aaccactgcg aacagggcaa
    21241 aattgttatt ccccggatgc gcgttgagcc ttccatcgac aacgaagagc agcatattgc
    21301 cgaaatggcg gcctttttcc gtaagcaggt ggagagcaaa aaacatctcg gtatgttggt
    21361 actgtttgcc agcggacggg cgatgcagcg ctttctcgac tatgtgacgg atttacgtct
    21421 gatgttgctg gttcagggcg atcagccgcg ttaccgttta gttgaactgc accgcaaacg
    21481 cgtcgccaac ggtgagcgca gcgtgctggt gggcttacag tcatttgccg aagggcttga
    21541 tttgaaaggt gatctgctca gccaggtgca tatccacaaa atcgcttttc cgcccatcga
    21601 cagccccgtg gtgatcaccg aaggggaatg gctgaaaagc ctcaaccgct atccgtttga
    21661 ggtgcaaagc ctgccgagcg cctcgtttaa cctgattcag caggttgggc gactgattcg
    21721 aagccacggt tgctggggcg aagtggttat ctacgacaaa cgcttgctga ccaaaaatta
    21781 tggcaagcga ctactggatg cattaccggt atttccgata gagcaaccgg aagtccctga
    21841 aggtatagtt aaaaagaaag aaaaaacgaa atccccacgc cgtcggcggc gttaatgatg
    21901 tgagtcaggt aaggagtcgt aaatggacta tcgcaaaatc attaaagaga tcgggcgcgg
    21961 gaaaaaccac gcgcgcgatt tagaccggga tactgcgcgc ggtctgtatg ctcatatgct
    22021 caatggtgaa gtccctgacc ttgagttggg cggcgtattg attgcgctgc gtatcaaagg
    22081 agaaggggaa gcagagatgc tcggctttta cgaagccatg caaaatcaca ccatcaagct
    22141 gacgccgcca gcaggcaagc cgatgccgat tgtcatcccc agttacaacg gcgcgcgtaa
    22201 acaggccaac ctgacgccgt tgttggcgat tctcctgcac aaactcggtt ttcctgtggt
    22261 ggttcacggg gttagcgaag atccaacccg cgtgctgact gaaaccattt ttgaattgat
    22321 gggtattacg ccaacgttgc acggcgggca ggcgcaggcg aagctcgacg aacatcaacc
    22381 ggtgtttatg ccagtcggcg cgttttgccc gccgctggaa aaacaactgg cgatgcgctg
    22441 gcggatgggc gtgcgtaaca gtgcacatac cctggcgaaa ctggcgacgc catttgccga
    22501 aggtgcggcg ctgcgtcttt ccagcgtttc gcatccggaa tatattggac gcgtcgcgaa
    22561 gttctttagc gatatcggcg ggcgggcgct actaatgcat ggtactgaag gtgaagtgta
    22621 tgctaatccg cagcgctgcc cgcagatcaa tctcattgac cgtgaaggga tgcgggtgct
    22681 gtatgaaaaa caggacactg ctggtagcga gttactgcca caagcaaaag atccggaaac
    22741 cacggcgcag tggattgagc gttgccttgc tggcagcgaa ccgattcccg aatcgctgaa
    22801 aatccagatg gtttgctgcc tggtggctat gggtgaagcg gcaactatca gcgacggcct
    22861 ggcgcgcgtt aatcaggcat tttaattctt ttctcagccg gatgagccat gtcatccggc
    22921 ttttacccca ccgcaatatg aaattcctgc atctttattg accttcccac gcccggcgtg
    22981 cagcataaaa atacaacaaa cacataacat aaacaggagt taaccatgga aagtggtcat
    23041 cgctttgatg ctcagacgct gcacagtttt attcaggctg tatttcgtca gatgggtagc
    23101 gaggaacaag aagcgaaatt agttgccgat catttaatcg cggcaaacct ggcagggcat
    23161 gattcacatg gtattggcat gatcccaagc tatgtacgct cctggagtca ggggcacctg
    23221 caaattaacc atcatgccaa aaccgttaaa gaggcggggg cggcggtcac gctcgatggc
    23281 gatcgcgcat ttggtcaggt cgcggcacat gaagcgatgg cgctggggat tgagaaagcg
    23341 catcagcacg gtattgccgc cgtggcgcta cataactcgc atcatatcgg ccgtatcggt
    23401 tactgggcgg agcagtgtgc agcggcgggg tttgtctcta tccactttgt tagcgtggtc
    23461 ggtattccaa tggtcgcgcc gttccacggt cgcgacagcc gctttggcac caatccgttc
    23521 tgtgtggttt tccctcgtaa agataatttc ccgctgttgc ttgattacgc caccagcgcc
    23581 attgcatttg gcaaaacccg cgtcgcctgg cataaaggcg tccccgtgcc gccaggttgc
    23641 ctgattgacg ttaacggcgt gccgacgacc aatccggcgg taatgcagga gtcgccgttg
    23701 ggttcgctgt tgacctttgc cgaacataaa ggctacgccc ttgcagcgat gtgtgaaatt
    23761 cttggcgggg cgctttccgg cggtaaaacg acgcatcagg aaacgttaca aaccagtccc
    23821 gatgccattc ttaactgcat gaccactatc atcatcaacc cggaactctt cggcgcgccg
    23881 gattgtaacg cgcagaccga agcctttgcc gagtgggtga aagcctcgcc acatgatgat
    23941 gataagccga ttttgctacc gggcgagtgg gaagtgaaca cgcgtcgcga acggcagaag
    24001 caggggattc cactggatgc gggaagctgg caggccattt gtgatgcagc gcggcagatt
    24061 ggtatgccgg aagagacgtt gcaggctttc tgtcagcagt tagccagcta aaaaaaagcc
    24121 cgtccagtgg cggacgggca aacaagggta acataggatc aatgagggtt agagcatatg
    24181 cgtctgtcgg caaacagaca gggaaatact tgtgctggac gtagcgtaaa cgcctgatcc
    24241 gtccaacggt tcggattttg taggcctgat aagacgcggt agcgtcgcat caggcatctg
    24301 ttgccggatg cggcgtaaac gccttatccg tcctacgaat tacttataaa ttaccgcagt
    24361 accgcttaat ttattgttgt tggtggcgga agtgatgctg tatccactgg caccggctgc
    24421 cgcggctttc tcagccagtt tcgcttccag ggcatcgagg gtggatgcgc catcggcaga
    24481 aaccacgccg attttattca tgttctgtgc ctgggatgcc gttatcggtt ccgccgcgaa
    24541 cacgccaaat gacagagttg aaagagccat agcagcaaca acagtattga tagttttcat
    24601 aattaatctc tcgcaggtga tttttgtata agtgacgttg tttcgtcgat gtgatgagta
    24661 tcacgttttt ttacgagaga taaaatcgaa gagaattgac cgccttgttc aaataaattg
    24721 attgatatct aacatactga taaaccattt atttgttgta cttatgaact tgttttatac
    24781 cgcgtggcag tcacagcagc gttaaaggca cggaatgcca cgcaaaataa aaattatact
    24841 cgctatgcat ctcgccagtt gtagttaacg taactggctg tctttcgaac ctctgcgatt
    24901 atatcctgta taagctggtt tttgtaaatc tttctcctgc tgacaatgaa tacataagcg
    24961 cacgccagga atggcttccc gacgggcctg cgggatgggg gcaccgcact cttcacattc
    25021 atccaggctt tcgccgcgcg gaatttcacc ccgagcgcgg gcaatcgcat cttcaattgt
    25081 actgttgatc tgttcgttga cggcgtcatc gttagcccaa ccggatgcca tatcgacctc
    25141 cccatatcaa tacttgtaca gttaagtgta gctaatccag ggacgaactc gggcagttca
    25201 agcatcagat ctccgaccat tcccgcagca gattatgata aagattaagc agcgacagga
    25261 tctcttcact ttcgccgtag cggcttttca gcgactgaat attgttgtcc agttcaaaca
    25321 gcatggcgcg ctttttatca tcgcggatca tcgactggat ccacataaat gatgccactc
    25381 gtacgccgcg ggttacgggt ggcacgcaat gctggctgct ggagggatac aacacgagat
    25441 cgcctgccgg gagttttacc cgatgttgtc cgaaggtgtc attaacgacc agttcgccgc
    25501 cgtcgtagct ttgtggatcg cttaaaaaca gcgtggcaga aaggtcagta cgcatccagc
    25561 cgttttgtgg atggctgcgt actgcgccat ccacatggaa accataggtt tcattgttct
    25621 gatagcgatt aaacagcggc gtggaaaggg tacgcggcaa ggccgcggca aagaacaaag
    25681 catgttggtt aaccgcgttc agcacctcat tttgcagggc ggcgtataac gtgctgcggg
    25741 tgtcgacctg ttgattgttc ttaacttgcg caccttgtgc gccggtggtg acgcgtccat
    25801 ccacccattc ggcttgttcc agttgttcgc gaaaccgggc gacgtcctgt ggcgataaca
    25861 cgccgggaat gtggtacatc atacaagttc tccaaaaagt ggggcctgcg ccccacatct
    25921 gaatcagaaa tgcatattgg ctgtgagcaa gaaggttctt ggctcgcccg ggtgataacg
    25981 gtagccgctc ttgttgattg aggcgacgta atcagtatca aacaggttgt agacgtttag
    26041 ctggaagtcg agattgcgat taacccgata ccccagtttg gcatcggcga cccagtaacc
    26101 ttcggtaaat gctggcgttc ccaccgcgcc gtctgaacct ttatgcatac tgccgatata
    26161 gcgtgcgccc gcgccaacag agatatcgtc ggttgcctga tattggctcc ataaggtgaa
    26221 ggcgtgctcc ggggtatacg gcagcgatga ggaaccatcc tgggcaacat ctttgccgtt
    26281 tttgatggtt gctttttgct gggtatagcc gccaatcacc tgccacgcgg gagtgatatt
    26341 cccggccacg gatatctcat agccttcgac gcgtttctta ccgtattgcg agtaagttcc
    26401 gtcatcattt tgctcaactt cattttcgat atcagtacgg aacagcgcgg cggtgagcaa
    26461 cagacgttta tccagaacct gccatttggt gccaatctcg ctggtgttgg ctttttgcgg
    26521 tttaaaatcg gtgcggttgg cactgttacc gctgccagac tgcgcaaggg cgaagttgtt
    26581 gccgcccgga ggctgctggg aaacggcata gttaatatag acattgccgt tttccgtcag
    26641 gtgatacagc gccccggctt tccagttcat cagattgccc gacttggcgg tgtcgacggt
    26701 ggtgaccgga gaaccttttg ccacaccagt tgggcaggtg atggcaccgc gtccgctgcc
    26761 gccgcaggcg gtggcactgt catattcagt atgataatta tccagacgga tcccgccgtt
    26821 cagctcaaaa tcacgggtga tttgcagcgt atcaaaggcg taaattgcga aggtatccgt
    26881 ctgaccattg gcgtttgcgc cgttgcgcgt caggccgccg ggatgaatgc tgctgtcagg
    26941 atgataaata tttaccgcgg gtaacgtcac cggattaacg ccgtagttag tctgcgtttc
    27001 acgggtaaat tccacgccgg tactgacatc atgaccgata gaaccggtat agaacgtcga
    27061 ggtcaggttg gtctggttgg tgagaatttt attactcaca tctttggtat tcgccgtgcg
    27121 tgaccaggtt cagctattca catcgctggt gggctgagta atattcgacg ccccgcccat
    27181 aatcgccgtc atcaggtaat cctgctttac gcgcgaccaa cgggtagtat tgcgaatggt
    27241 ggtgttatcg ttgatgtcgt gctcaaaacg catggtggcg gtgtcggtgg tcgaatcgtc
    27301 gtaatcggaa tccgtgccgt aaaagttatg agtatcaact tttccggaat gattcagggc
    27361 cgccgttccc gcagatgggg cagaatagcc cggcaaaccg atggtcggaa tgccgccgtc
    27421 tggcgtgttg tgctgggtga catgcagata attaagatac aaacgattcg ctgtaccaag
    27481 gccaaaagcg acagaagggg cgacgccgta acgctcattt ttgactttgt cgcgtccggc
    27541 atcgtgcgtt ttttcgccca ttacattcag gcgcaccgcg gtggtatcac caatgacctg
    27601 attgacgtcc agcgtgccgc ggcggaacca ggcgctgcca atactggcgg aggcgtcaat
    27661 gccggaatca ttgcgcggct gtttgctgat catattgatc gagcccgtcg gtgcgctgcg
    27721 cccgtagtcg gtgccggacg gccctttaat cacttcgacc tgctcggtat tgaaggtgtc
    27781 gcgcgagacg ctgccgatat cgcgaatgcc atcaatataa atactgttag aggtatcggc
    27841 accacgcata taaatggcgt cgccagtggt ggagttaccg ttctcacccg caaaaaacgc
    27901 acccacgc
//
LOCUS       DAZPLI010000041        27740 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZPLI010000041 DAZPLI010000000
VERSION     DAZPLI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27740
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.041"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(12..87)
                     /locus_tag="ACHUU3_001701"
     tRNA            complement(12..87)
                     /locus_tag="ACHUU3_001701"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52..54),aa:Lys,seq:ttt)
     gene            complement(91..166)
                     /locus_tag="ACHUU3_001702"
     tRNA            complement(91..166)
                     /locus_tag="ACHUU3_001702"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(131..133),aa:Val,seq:tac)
     gene            complement(316..391)
                     /locus_tag="ACHUU3_001703"
     tRNA            complement(316..391)
                     /locus_tag="ACHUU3_001703"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(356..358),aa:Lys,seq:ttt)
     gene            complement(556..1347)
                     /gene="cpoB"
                     /locus_tag="ACHUU3_001704"
     CDS             complement(556..1347)
                     /gene="cpoB"
                     /locus_tag="ACHUU3_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR2081792.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1357..1878)
                     /gene="pal"
                     /locus_tag="ACHUU3_001705"
     CDS             complement(1357..1878)
                     /gene="pal"
                     /locus_tag="ACHUU3_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR2081793.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1913..3205)
                     /gene="tolB"
                     /locus_tag="ACHUU3_001706"
     CDS             complement(1913..3205)
                     /gene="tolB"
                     /locus_tag="ACHUU3_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR2081794.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3338..4570)
                     /gene="tolA"
                     /locus_tag="ACHUU3_001707"
     CDS             complement(3338..4570)
                     /gene="tolA"
                     /locus_tag="ACHUU3_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR2081795.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            complement(4635..5063)
                     /gene="tolR"
                     /locus_tag="ACHUU3_001708"
     CDS             complement(4635..5063)
                     /gene="tolR"
                     /locus_tag="ACHUU3_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR2081796.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5067..5759)
                     /gene="tolQ"
                     /locus_tag="ACHUU3_001709"
     CDS             complement(5067..5759)
                     /gene="tolQ"
                     /locus_tag="ACHUU3_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR2081797.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5756..6160)
                     /gene="ybgC"
                     /locus_tag="ACHUU3_001710"
     CDS             complement(5756..6160)
                     /gene="ybgC"
                     /locus_tag="ACHUU3_001710"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR2081798.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6310..6603)
                     /gene="ybgE"
                     /locus_tag="ACHUU3_001711"
     CDS             complement(6310..6603)
                     /gene="ybgE"
                     /locus_tag="ACHUU3_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR2081799.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6603..6716)
                     /gene="cydX"
                     /locus_tag="ACHUU3_001712"
     CDS             complement(6603..6716)
                     /gene="cydX"
                     /locus_tag="ACHUU3_001712"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR2081800.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6731..7870)
                     /gene="cydB"
                     /locus_tag="ACHUU3_001713"
     CDS             complement(6731..7870)
                     /gene="cydB"
                     /locus_tag="ACHUU3_001713"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR2081801.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7886..9454)
                     /gene="cydA"
                     /locus_tag="ACHUU3_001714"
     CDS             complement(7886..9454)
                     /gene="cydA"
                     /locus_tag="ACHUU3_001714"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR2081802.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10302..12935)
                     /gene="mngB"
                     /locus_tag="ACHUU3_001715"
     CDS             complement(10302..12935)
                     /gene="mngB"
                     /locus_tag="ACHUU3_001715"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFR2081803.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(12953..14869)
                     /gene="mngA"
                     /locus_tag="ACHUU3_001716"
     CDS             complement(12953..14869)
                     /gene="mngA"
                     /locus_tag="ACHUU3_001716"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR2081804.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            15038..15760
                     /gene="mngR"
                     /locus_tag="ACHUU3_001717"
     CDS             15038..15760
                     /gene="mngR"
                     /locus_tag="ACHUU3_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR2081805.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15864..16733)
                     /gene="sucD"
                     /locus_tag="ACHUU3_001718"
     CDS             complement(15864..16733)
                     /gene="sucD"
                     /locus_tag="ACHUU3_001718"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR2081806.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16733..17899)
                     /gene="sucC"
                     /locus_tag="ACHUU3_001719"
     CDS             complement(16733..17899)
                     /gene="sucC"
                     /locus_tag="ACHUU3_001719"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR2081807.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(17993..19210)
                     /gene="odhB"
                     /locus_tag="ACHUU3_001720"
     CDS             complement(17993..19210)
                     /gene="odhB"
                     /locus_tag="ACHUU3_001720"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR2081808.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(19225..22026)
                     /gene="sucA"
                     /locus_tag="ACHUU3_001721"
     CDS             complement(19225..22026)
                     /gene="sucA"
                     /locus_tag="ACHUU3_001721"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR2081809.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22327..23043)
                     /gene="sdhB"
                     /locus_tag="ACHUU3_001722"
     CDS             complement(22327..23043)
                     /gene="sdhB"
                     /locus_tag="ACHUU3_001722"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR2081810.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(23059..24825)
                     /gene="sdhA"
                     /locus_tag="ACHUU3_001723"
     CDS             complement(23059..24825)
                     /gene="sdhA"
                     /locus_tag="ACHUU3_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR2081811.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24825..25172)
                     /gene="sdhD"
                     /locus_tag="ACHUU3_001724"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24825..25172)
                     /gene="sdhD"
                     /locus_tag="ACHUU3_001724"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR2081812.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(25166..25570)
                     /gene="sdhC"
                     /locus_tag="ACHUU3_001725"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(25166..25570)
                     /gene="sdhC"
                     /locus_tag="ACHUU3_001725"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR2081813.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            25951..26058
                     /locus_tag="ACHUU3_001726"
     CDS             25951..26058
                     /locus_tag="ACHUU3_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2081814.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26264..27547
                     /gene="gltA"
                     /locus_tag="ACHUU3_001727"
     CDS             26264..27547
                     /gene="gltA"
                     /locus_tag="ACHUU3_001727"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR2081815.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT         6716 a         7496 c         6772 g         6756 t
ORIGIN      
        1 gatgttaaaa ttggtgggtc gtgcaggatt cgaacctgcg accaattgat taaaagtcaa
       61 ctgctctacc aactgagcta acgacccgag tggtgggtga tgacgggatc gaaccgccga
      121 ccccctcctt gtaagggagg tgctctccca gctgagctaa tcacccgata ctgcgctgga
      181 tactacaaac tacttccacc gaacccaccg taattagtgg tgggtcgtgc aggatgactc
      241 gcttcgctcg ccctacgggc cgttgctgac gcaacgttat cctacacgct taacatctgt
      301 cttcgatgct aaaactggtg ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag
      361 tcaactgctc taccaactga gctaacgacc cactttttcg ttgctttcgg tttgtttgat
      421 atcccgtggc aacggcggca tatattactg atttcagact tgagcgcaac aaaaatttcg
      481 atgcagatca ctcaactgct tatgattcgc acgacacgac cagaaataat gcgacttctg
      541 gtcgtgtgtt atgcattaca tcgcgttcag acgtttttgt gcctgtttag cgccatcagt
      601 accagggtat ttactgataa cctgctggta tacggctttc gcttttgcgg tgtcaccttt
      661 gtcctgcatg atgacgccga ctttaaacat cgcatcagca gcctttggtg acttcggata
      721 gtttttcact accgaagcaa aatagtacgc cgcatcatct tttttaccct tgttgtagtt
      781 taactgaccc agccaataat tggcgtttgg caggtaagtt gaatcagggt aatttttgat
      841 gaaattctga aatgccacca ttgcgtcatc ctggcgggat ttatcctgca ccagcgcaat
      901 agctgcattg taatccgtgt ttgcatcgcc gcttttcacc ggcgcgccag cattcgcagt
      961 accagcatca gctgtcggcg tcgttgatgc cgccgcaccg ctttgatcgc tgctggttga
     1021 ttgcgccgct gcaccaccgc tgctgaggct gtcgatctgc aacaggatct gcttttgccg
     1081 ctccacgacc tgattcagtt gatactgatt ttcctgaatc tgaccacgca gggaatcaat
     1141 atcggattga ttatcagaaa gttgttgctg gagttgggtt aaaagctggc tgtgagcatt
     1201 agaaatacgc tcaagttgag tgacgcggtc ttcgaccgag cctgagccga cactactgat
     1261 tggtgcctga gcaaaagcgg cccagggggc cgctatacca accagtaacg acagactcaa
     1321 tagttgatgt ctgaagttac tgctcatgca attctcttag taaaccagta ccgcacgacg
     1381 gtttttggag tatgccgctt cgtcatgacc cagtactgca ggtttttctt taccgtaaga
     1441 aacgatggag atctggtctg cagaaacgcc tttaccctgc aggtacatct taacggcgtt
     1501 cgcacgacgt tcacccaggg agatgttgta ttccggagta ccacgttcgt ccgcgtgacc
     1561 ttctacggtg actttgtaag acgggttgct acgcaggaag tttgcatgtg catccagcat
     1621 ttgagcgaag tcagaacgga tatcgtactt gtccagatcg aagtaaacga tgttgttctg
     1681 ctgcagctgt tgcatttgca gacgagcctg ctcttcggaa gacatgttgc cgttgccgcc
     1741 gttcgcatcc ataccagtgc cggcacccag catgccttcg ctgccgtcat tgctggcgtt
     1801 cttgttggaa gaacatgccg caattgccat aacaggcaga gcaatcatca gccctttcag
     1861 cactttgttc agttgcattt caatgattcc tttactattc aattaattat tatcacagat
     1921 acggcgacca ggcagggaat ttgacctgtc catcagttgc cggaagacgc gctttgaaac
     1981 gcccatctgt agaaaccaaa ttcagcacgg atcccatccc ctgagaagag ctgtagatta
     2041 ccatagtgcc gttaggtgcc agacttggcg tttcatccag gaacgtggac gacagaactt
     2101 gtacgcctcc cgttgccaga tcttgtttgg caatgtgctg ctgcccaccg ttggagctga
     2161 ccattaccat aaatttaccg tcgctgctga catccgcatc ctggttctgc gaaccttccc
     2221 aggtaatacg ttgtggcgca ccgccgttga tattcacttt ataaacctgt ggacgaccgg
     2281 cctggtcaga agtaaatgcc aggttctggc tgtccgggaa ccaggtcggt tcggtattgt
     2341 tactgcgacc atcagtcacc tggcggatct gaccagaagc caaatccatt acgtacaggt
     2401 tcaggctacc ggttttcgac aaggcgaatg ccagtttgct gccgtctggc gagaatgcag
     2461 gcgcaccgtt gtgacgcggg aatgaagcca cctgacgtac agcgccgttt gccagcgttt
     2521 ggataactag cgcggaacga ccgctttcga aggtcacata agccagttta gaaccgtctg
     2581 gtgaccacgc cggggacatc agcggctgtg gtgaacggtg aacgacaaac tggttgtaac
     2641 cgtcatagtc agatacgcgc agttcatacg ggaactgacc gccgttggtc tgaacaacgt
     2701 aggcaatacg ggtacggaac gcacctttaa tgccggtcag tttttcaaac acttcatcac
     2761 tggcggtatg accagcatag cgcagccact gcttgttcac tttgtacgag ttctgagcaa
     2821 gtacagtacc cggtgcgccg ccagtgtcaa caagttgata agcaacattg taagaaccat
     2881 ccggattcgg agtgacctga ccgacaacta cagcgtcaat acccagcgcg gaccatgcag
     2941 ctggttgtac ttcctgcgca ctacccggct gctgtggcag acgagcgcga tctaacggat
     3001 taaatttacc gctgttacgc aagtcggcag caacgatgcc gccaatatct tcaggtgccg
     3061 caccagggcc cgcccactgg aaaggaacaa caccaatagg acgaccggaa tctacaccgc
     3121 tgtcgatcac aatgcggact tcagcatgca gaactgatgc ccacagtatg agaaaaccaa
     3181 atgctactcg taatgcctgc ttcatcatat ctcccttatc tggaccgatg gcccacgata
     3241 atttagcaga atgttaacaa actaaaatac acaaaactac cagaaccccg tggcaaccgg
     3301 tgcctgatgt tgaccgtcgg aacagtcaac atcgcgatta cggtttgaag tccaatggcg
     3361 cgtttttgaa cacttcatat actgcctggc ttggtggttt cgggatcttc gcaagtttag
     3421 ctgctgccaa cgcagcctga caaagtgcgg gatcgccacc ttcaggtttg atatccagta
     3481 acataccatc gggtgccagt tttatgcgca gcgtacaggt tttgcctgca taggacgatg
     3541 cgtcatagaa cttactttcg atagcagatt taatctgccc ggcatagtta ttgatatcgg
     3601 cccctgatgc gccattgttt ttagtattac cactcccggc aggcgaagca ttgttccctt
     3661 tcgcccctcc ccccgttttc ggtgcattct taccagagct tagctcaccg aaaatatcat
     3721 ctgcctctgc ggcagctttt gctgcagcgg ctttttcagc tgctgctttt gccgctgccg
     3781 cttttttgtc tgcggcggct ttttctgccg ctgcttttgc cgctgccgct tttttgtctg
     3841 cggcggcttt ttctgccgct gctttcttat cagctgctgc cttatcagca gccgcttttt
     3901 ctgctgcttt tttgtcggct gcagctttct ctgctgccgc tttctttctt gcttcagctg
     3961 cagcagcttc tgccgcttcc gctttcttct tcagtgccgc agcggctgcc tcggcttttt
     4021 tctgcgcttc ggctgcggct ttggcggctt ctgcttctgc ttttttcttc gcgtctgccg
     4081 ccgctttctt cgctgcttct tccgcagctt tagcatccgc ttcggccttc gctttagcat
     4141 ctgccgccgc tttcgctgcc gcctcttccg cttgcttctg ctttaactcg gcctgttttg
     4201 cggcttcttc agcctgcttt ttctgttcct gagcagctaa ccgctctttc tcaagttgct
     4261 tcaggcgttc ctgttcagcc gcttgtttct cacgcagttc ttcagcagcc tgctgttcct
     4321 ttatcttgcg ctgctcatca gaacgcttcg cgcttgattc ctggctttgc atgcgtttgt
     4381 actgctcaac taccgcacct gaatcaacca tgacagcgtc gatggacgaa ccaccgccgc
     4441 ctccggctga agcttctata ttctcatcga acgaactcca gagcagcgcc gcaaataaga
     4501 tgacatgcag cactgctgaa ataattatcg cccgcttgag cttgtcgttt tgttcggttg
     4561 cctttgacac tctcggtttc caaaaactgt tcgcctgtta cccgctctct ttcaagcaag
     4621 ggaaacgcag atgtttagat aggctgcgtc attaaaccaa ccgatttcac acccgcacta
     4681 tgtaacaagt tcagtgcttt aattatttca tcgtaaggca catcttttgc gccaccgatc
     4741 agaaagaccg ttttcgggtt ggccttgaaa cggctggaca cttccgccac cacctgctct
     4801 ggtggtaaac gctccaggcg atctttctca accaccacgg tgtactgacc aataccagac
     4861 acttcaacaa tcactggcgg attatcgtta ctgctcaccg cctgtgattc agtagcgtct
     4921 ggcagatcga cctccacgct ctgggtgatg atgggcgctg tcgccataaa gatcagcaac
     4981 agcaccagca gtacgtccag caacggtaca atgttgattt cggacttgag atcgcgacga
     5041 cctcgtccac gcgctctggc catggcttac cccttgttgc tctcgctaac ggtaaacgcc
     5101 tggcggtgca gaatcgcggt aaactcttcc ataaagttgt cgtaattcag ttccagtttg
     5161 tttacgcgct ggttgaggcg gttataagcc attaccgccg gaattgcggc gaacagacca
     5221 attgcagtcg caatcaacgc ttctgcgata ccgggcgcaa ccatttgcag tgttgcttgt
     5281 tttaccgccc cgagggcgat aaaggcgtgc atgatccccc agaccgtacc aaacagacca
     5341 atatacgggc tgatggagcc aaccgtaccg aggaacggaa tgtgcgtttc cagattttca
     5401 agttcacggt tcatggagat acgcatagca cgcgacgccc cttccactac ggcttccggc
     5461 gcatggctat tggcacgatg caggcgcaca aactctttga acccgctgta aaatatttgt
     5521 tccgaaccag tcagattatc ccgtttcccc tgactctctt gatagaggcg agagagttcg
     5581 attccagacc agaatttatc ttcaaacgct tcggcttcgc gcgccgctgc gttaagaata
     5641 cgggtccgct ggataataat ggcccaggat gcgattgaaa aaccaatcaa aatcaacatg
     5701 ataagtttaa ccagaaggct agccttcagg aacaaatcaa ggatattcat gtcagtcact
     5761 gcttaaactc cgcgacaata gacttgggaa gcgcacgagg cttcattttg agtgggtcaa
     5821 cgcaaacaac cagaacctct gcttcattca gcaaagtatt ctcggcgttg acaatacgtt
     5881 gcgtgaaaac caaagaggtg ccacgcattg atgttatttc agtctgtatt tcgagcatat
     5941 cgtcgagccg cgcaggtgcg taatattcca ccgtcatttt acgtaccaca aaggcaacgc
     6001 gttcagccat cagcgcctgc tgactgaagt gatgatgacg cagcatctct gtgcgtgctc
     6061 tttcataaaa agcgacgtaa ctggcgtggt acaccacacc accggcatcg gtatcttcat
     6121 agtagacgcg aaccggccat cgaaacagcg ttgtattcac tttacatccc ggtaatgcaa
     6181 caaaagttag agcttttaaa cgctgctact atacgcgcgc gattgaggtt tgggaatgag
     6241 ggcaagttaa gggagcgtaa aaatttatgg gcctggagcg gcccataatt ttttaaagag
     6301 aattctgatt taaaagaaga agaaaatcag cccgacaatg agaacaatat cggcaagcaa
     6361 tgggcaaaaa atcccttgcc aaagaacctt ctgcggacga aagcccacgc cgtgaatcac
     6421 accagcacag acggcccaca tcagcaataa accatgccag atttccagtt cactggtctt
     6481 cgcggcaaaa cgagatgggt cccaaaacat acatcctgct aacagaagcg ccatcacgaa
     6541 ggaaagcgcc cgcagggggc gcttgtccat taccgcatac aaggtcgcga taatcttact
     6601 catcagatgt cttcttgacc ggctttgcct gattcgacgt gttcaagcgc cagcgcggtg
     6661 attaccccaa acgaacaggc aagaagcgtt cccagaatcc atgcgaaata ccacattttt
     6721 agctccttac ttagtacaga gagtgggtgt tacgttcaat atcttctttg gtgatacgac
     6781 cgaacatttt ccagtaacac caagcggtgt agagcagaat gatcggtacc agaaccaccg
     6841 caacccaggt catgacgtta agcgtcagct ggctggaagt tgcatcccac attgtcagac
     6901 ttgcgttcat catggtgctg gacggcatca caaacgggaa cattgcgata ccggctgtca
     6961 ggatgatgca ggccagcgtc agggaggaga acacaaacgc ccacgcggct ttatccatac
     7021 gtgcagtcag gatggtcagc agcggaagaa ccacacccag tgccggaata gcccacagaa
     7081 ttggcgtgtt gttgaagtta accagccatg cgccagcttc acgaaccact tctttattca
     7141 gtgggttaga ggctgcgtaa tggtccattg tcgatttcac gacataacca tcgataccgt
     7201 acatcaccca tacgccagcc agtgcgaaac agaccagtgt caccagcgca gccacctgag
     7261 ccgttgcacg ggtacgcagg tgcagttcgc ccacggtacg catttgcaga taggttgcgc
     7321 cctgagtaat gatcatccct acgctcacca cgcctgccag caggccgaac gggttaagca
     7381 actggaagaa gttaccggtg tagtacagac gcagatattc atcaacgttg aacggtacgc
     7441 cctgcaacag gttaccgaac gctacaccaa ttaccagcgg cggaacgaaa ctaccaatga
     7501 agatgcccca gtcccacatg ttacgccagc gggtttcttc aatcttggag cggtagtcaa
     7561 aaccgaccgg acggaagaac aaagacgcca gcacgaggat catcgccaca tagaagccgg
     7621 agaacgcagc ggcatagacc atcggccagg cagcaaagag tgcgccgccc gcggtgatca
     7681 gccaaacctg gttaccgtcc cagtgtggtg caatggagtt aatcataatt cgacgctcgg
     7741 tgtcgttacg accgaggaaa cgggtgagca tgcccacccc catgtcgaaa ccgtcagtga
     7801 ctgcaaaacc aatcagcaga acgccaacca gcagccacca gataaaacgc aatacttcat
     7861 aatcgatcat ttgacgactc ctgtcttagc gtgccggctg agtagtcgtg gaagactgct
     7921 caaagtgata gcgaccggtt ttcaggctgc ttgggccgag gcgtgcaaac ttgaacatta
     7981 agaacaattc tgccaccagg aacagggtat acaggccgca aatcagcacc attgagaaga
     8041 tgagatcgcc tgcggtcagt gacgagttcg ccacagctgt cggcagcact tcaccgatag
     8101 cccacggttg gcggccatat tcagccacga accagcccgc ttctacagca atccacggca
     8161 gcggaatacc gtacagcgcg gcgcgcagaa gccatttttt ctcgccaatg cggttgcgga
     8221 tgacactcca gaaagagagc gcgatgattg ccagaagcag gaagccacac gccaccatga
     8281 tacggaacgc aaagtacagc ggcgctacac gcgggatgga gtctttggtt gcctgttgaa
     8341 tctgcgcttc agtcgcatca gccacgtttg gcgtatagcg tttcagcagc agaccgtaac
     8401 cgaggtcttt cttcatgcta ttgaactggt cacgaaccgc ctggtcggta gaaccagaac
     8461 gcagttgttc gagcagagag tacgccttca tcccgttacg aatgcgttct tcatgctgca
     8521 ccatcagctc tttcaggccg ataaccgggg tatccacgga acgcgttgca atgatgccca
     8581 gtgcgtaagg gatctgaatc gcaaatttgt tcgtctcctc ttcctgatca ggaatgccga
     8641 acagagtaaa ggcagcaggc gcaggttgcg tttcccactc ggcttcaata gcagccagtt
     8701 tggttttctg cacgtcgccc atttcgtagc cggattcatc acccagaaca ataacagaca
     8761 gaacagcagc cataccgaag ctggcagcga tagcaaagga gcgtttagcg aaggcgaagt
     8821 cacgaccttt cagcatatac catgcgctga taccgaggat gaacatcgcg ccagtcacat
     8881 aaccagacgc tacagtgtga acgaatttca cctgagcaac cgggttaagc accagctcgg
     8941 agaagctcac catctccata cgcatagttt caaagttgaa atcggacgcg attgggtttt
     9001 gcatccagcc gttcgcaacc agaatccaca gtgcggacag gtttgaaccg agcgccacca
     9061 gccaggtgac acacatatgc tgaactttac ccagacgatc ccaaccgaag aagaacagac
     9121 ctacaaaggt ggattcgagg aagaaggcca tcagaccttc gattgccagc ggcgcaccga
     9181 agatatcccc tacatagtgg gaatagtaag accagttagt cccgaactgg aactccatgg
     9241 tcagaccggt agccacaccc agagcgaagt tgataccaaa caacttgccc cagaacttgg
     9301 tcatatcttt ataaatctgt ttgccggaga ggacgtagac cgtttccata atggccagca
     9361 ggaacgccat accgagcgtc agtggcacaa aaaggaagtg gtacatcgcg gtcaaggcaa
     9421 actgtaagcg cgacagttcg actatatcta acatcatgac tccttgctca tcgcatgaag
     9481 actccgagag tgaaccccgt tagaaagggt cacacgcatg ccccaataga aatttgtttg
     9541 aacactttca tcataccatg tgaatgcaca caaaaacggc gtaaagaaag gttgcaaaca
     9601 tgttaataaa aactcaaatt gatcccacgt atatattacg ccgcaaaatc cttacaataa
     9661 acaggttttt gttgaagcaa atttgcattt ttcgacggtg atcaatttat agctaaatta
     9721 ccgcctttca gccaatttga tcgagaacaa tttatctctt tttgatgcca atttccaaga
     9781 cttatacatt gataaatatc aattcctaca aattatgtta atcatgtttc aataaggtaa
     9841 ctttagtgac atttatgttt aaaatgtgtg agttataagg ctgaaattag tttcggttgg
     9901 caatttaaag ataaaaagag acgaagcaaa aacagcacgg ttgaccttaa ttcttttttt
     9961 ggtcactggc taatacgacg cagagtttca ggattactaa taaaggcata aatccacccc
    10021 gccgatatta acatttaatt aacttgtaat tatattataa ttaactcacc cagggttatt
    10081 attttgttaa aaataaagcc acatatacgt ggcttgagag tgaagataaa cgcaaaatta
    10141 cctgatgcgc tacgcttgcc agtgtctaca tcatctctgc aatatattga atttgcctgc
    10201 ttttgtagac cgaataaagc gttcatgccg tatccggcat gaacaaagcg ccctttgtca
    10261 acaatctggc cgcgcatatg tggcttcggt ttgattgcta ttcaggcaag ccggtaactg
    10321 aacgtccgtg actggccggg taaaaaaggc cccgatagat ttgaaccttg attttcctcg
    10381 gtggtaatgt gttcatccat catcgtttct gagcaagaaa tcacctcgcg actgaaagca
    10441 acagtcgcat cacaggttgc tgattcagcc ggattaaaca gccgtaaaat cacttcttgt
    10501 cggtcttcag ctttcttaag tgcgcttatc aggcatccca ctgggggcat tttcaacaaa
    10561 ctataacttt ccggcacgtt gaatccggct ttgttgagct tcatcgcatc ccatgggatt
    10621 ttgttgtaac actgtactgg agtcagccat gctcgcgcct gctgagctac accagcggcg
    10681 gttggcgtac cggtataact caataaactt aggcgacaag aaagcagacc acgtagttgt
    10741 gagtccggga ctggcatttt aattcccgaa ggccgcccag gccttaaaag cagatcttct
    10801 ttgcccagta agcccacgcc acgcagcaac gtaatggcaa aggttttctt ctcttcaccg
    10861 atgacttcaa attcacgtaa cccttcgcta aagacagcca tgccgttacg cccttcctgt
    10921 aaggcaacat agttgagcat attccatacc ggaaccggcg cttctttcca gccttcttgc
    10981 tgccagttgt tcattgcact gtcgttcacg gggcgcgtta gcgaaccaaa ctgcgtatct
    11041 gccagaacac tgtcagtgtt aaaaggtgta gggaccagga cacgaaggcg atgatcgtca
    11101 gcctggttat caaggttgat atccacatca atacgcctgc tgttatgact aagagtgaca
    11161 accaacaccc cccctactct gccagtggat tgccgggcgc tgcgttctga caaattgagc
    11221 ggcactgcca tgtcatagcg gataacagcc ctgctctgcc aggcttcatg aataatatcg
    11281 cattgcggtt tcgcgttcgc tgcggtaatt acccactctt cttttgcggg tgaatagtca
    11341 tattcatcac catcatcaga gctttcttca atttgcaata cccggtcata gcgcacaccg
    11401 ctgtctttat ctaccagttg cagagaacca tcctcattga gcgcaatctg ccagaaagca
    11461 ttttccagaa tcccttcagc gtcacttttt gccgcaatca cgttgccagg ctgattcgct
    11521 tcaatataaa gcgtgcgata gcccatagaa gggacaatct ggttgatctg aatatcaaac
    11581 tccataaagg gatcataatt gccataatga acaatttgcc gatcgattag gccaggatcg
    11641 atctcacggg catggcgaat aaaatacggt acaggctgac cgcgatcgtc ttgtagatta
    11701 aactggctgg cgcgcagccg cacagtggtg ttaataactt cttcccgcgg ccagggcatc
    11761 aggttaaaca gcacgagttt gtcggcatca ctctgtggca tgttatcggc aattttgcgc
    11821 atgtagaaac gcagcagatt atccgccatg tcttcagcca gttcgaagcg ggcgacaatt
    11881 tcgcgatgaa ctttgtcact gcaacaacaa ccgatactgt cgtgggcatg attttttaag
    11941 atctctttcc acattttctc cagcaagccg tggtggtatt caaaacccaa cgtccaggcc
    12001 agcgttgcca gcggttccag cagattaaca atcttatttt caatacgtgc gtgggcaatt
    12061 ttgatatcca tgcgcgtgga accgatggtg cgatgtacgc gcatatattt gccatcgata
    12121 aattcacctt tcagggtggc cagactttca cgctgcgctt cgatcttctc aaatacctct
    12181 tcaaagcggc tcatcacaaa cttacgttga gggtagatct cacgtagctt atccatcact
    12241 tcgaagatat tttgctgcaa tggcatctgg tcatgaccat tcggcagcaa aatctctttg
    12301 gttacagacg ctttttccag cacgtcaaaa taactgtcga ggcgtttacg taatccgttt
    12361 tcgtcggcag gtaagtactt accgatggcg tagcccagcg gcagcacctg cgccgtcact
    12421 tcgctaccgt cactgctttg ccacaaaaac tcggttttat cagtaccgtg gcgctccgaa
    12481 catccgcgcc agaacatagt gcgggtaatg ccaaatccat tgtaaatatg cggcagttgc
    12541 ccggacatgc caaaggaatc cggtaaataa cctattttca tcggctcgcc aaacgcgagg
    12601 cagtcacgca ttccgtacat cagattacgg acgatggatt ccgcagaaac aatcgtggta
    12661 tcggtctggg tataccaggg gccgataatc aacttaccgg cttctacctg tttcttcacg
    12721 cggtctttgt tttccggttt caccgcgaaa taatcttcga ggatcgccgt ttgcccgtcg
    12781 agtacgtaat atttgtattc gttgtcctgt tccagtcggc acaggatctc ttccatatta
    12841 ttgaccagca gaatgcgtga ctcttcggtg gtgaaatacc actcccgatc ccaatgcata
    12901 tgcggggtga tgtgaacgcg agataccgct ttcatcgtcg tttccttttt gtttatggca
    12961 ttacgccatc agtcagatag ttgccatgct taaccgcgtg acgccgccag atcaggagaa
    13021 ttgcagtcga gattgcagcc cccaccaatg ccgcaccaaa ccagccaatt gccgccataa
    13081 caccgcccgc gccattatca tgaagtaaaa agagcgagaa aatgccggca ccgggtgtcg
    13141 aaaggccgat attcatcgca ccgacaatag cgcccgttac catagagccc agcacaaacg
    13201 aaccaataac ccgcagcgga tcttcaatcg ccatcgggat cgccccttcg gtaatacctg
    13261 ccagccctaa cagccaggtg gatttcccgg tctcaatttc aaactcttta aacaggcgcg
    13321 gtgcgagcat cgtggaagcg gttacggtaa atgccgaaac cattttgacg gaggcgaaaa
    13381 tggcatacgg gccgtaaacg ccgttcgcca ttgcgcccag gcagaatgca taagcggctt
    13441 tgttcactgg ccctccaagg tcaaaggaac acataaaacc gagaatggca cccagcaaca
    13501 gcgcgttact tcctgacaga ccgttcagcc aggcggtaag cgagttattg atccatgcga
    13561 caggttctcc caccacaaac agcatcagac tgcccgctcc caacgtaccg agcaccgggt
    13621 agagataaaa agtcaggaat ccattgaatt tactgctaag acgcaagtga tttttcaccc
    13681 agcgcatcaa gtaaccggct atcaatccgc caacgaccgc gccgagaaac ccggagccga
    13741 tcatgttggc ggcaagtcca gccgcaaagc ctggcgctaa cgccggttta tctgccagag
    13801 aataggcggt ataggccgcg agcaccggta ccatcaaaat tccgagcagc ccgccgccca
    13861 gcttgcggta catccacagc caggagtttt cttcattaaa cagatcttgt agcccgaaga
    13921 tttgcgacag taataccgcg accgccagca ccgtgccccc cgcgacaatc aacggtacgg
    13981 caaaagagat tccgctcaac agtgcctgtt tcagctccgt tttgacactt ttcaccggtt
    14041 gcgtatcttg ctgtacggta cgcgtctcat cgctacgctt gagggtaagc gcttgttgga
    14101 tcaacgcttc tgcatggcga atcggctcgg caacaggcac tgaaagcgcg ggaatgccat
    14161 taaaacgctc actctccttg atggcgactt cagccgcaaa aatacaggcg gttgcactat
    14221 tgagttgatc cgccgttaaa cgcccttcaa tgccgttagc gccttgtttt tcaacgtaaa
    14281 catttacgcc gagtttgcgt ccggcttttt ccagatattc cgcagccata taggtgtgag
    14341 caatacccgc cggacaggcc gttacgcaga cgatagttgg cgcgttggaa aaagaggcag
    14401 aaggttgcgt gcctcccttg tcatccagcg ccgagagcaa ctcatcaggc gtcgtcgccg
    14461 actgtatacg cgcccgaatc tcatcatccg caaggcgcgt ggtcagcgct gtcagcagtt
    14521 gcatatgcgt agtacccgct tcattggggg gaatcgccag cagcaccact aaatcaactg
    14581 cttccgggcc atcaacgcct tcccactgaa gcggctcgct gagtgtggcg accgcaaacg
    14641 ccgcttcttt taccgcagca gttttgccat gcggcacagc caacccttca cctaaggcgg
    14701 tcgggccaag gctttcacgg cgatacactt cttccagaaa ttgctcagta ctggaaattt
    14761 tccccagagc agcaagacgt tgagtcaacg cgtggatggc ctcttcacgg ctggtaaagc
    14821 gcgcattcag acacaacgca tcgcggtggg tcagagtcgt cagattcatc atgatcctca
    14881 cttgatcgtt tttatagtcg cgccagtgtg cccgataaaa caataccatc atgtgatcaa
    14941 tttcacttga ttgaaattat ttgtattgta tttgtattat ttacaggatg aatttatcgg
    15001 gcataatggc taaattcgta ttaatgagat actaaaaatg ggacacaagc ccttataccg
    15061 gcagattgcc gatcgcattc gtgaacaaat tgcgcgtggc gagcttaaac ctggcgatgc
    15121 gttacccact gaatcagcgc tacaaacaga gtttggcgtc agccgggtta cggtgcgtca
    15181 ggctttgcgc cagctagtcg agcagcagat cctcgaaagc attcagggca gcgggactta
    15241 cgtcaaagaa gagcgggtca attacgatat ttttcagtta accagttttg atgaaaaact
    15301 gtcggaccgt cacgtcgata cacacagtga aatcctgata tttgaagtga ttccggctga
    15361 cgattttctt cagcaacagc tacaaatcac cgcgcaggat cgtgtgtggc atgtgaagcg
    15421 cgtgcgctat cgcaagcaaa agccaatggc gctggaagaa acctggatgc cgcttgcgtt
    15481 gttcccggat ctcacctggc aggtcatgga aaattcgaaa tatcacttta ttgaagaagt
    15541 gaagaagatg gttatcgatc gtagcgaaca ggaaatcatt cctctgatgc caaccgaaga
    15601 gatgagccgt ctgcttaata tcagccagac aaagccgatt cttgaaaaag tatcgcgtgg
    15661 atatttagtt gatggtcggg tgtttgaata tagccgcaac gcttttaata ccgatgacta
    15721 taaatttacc ctgatagcgc agcgaaaatc atcgcgataa gcacaaaaaa ggccatcata
    15781 cgatggccaa ccatgtcgaa accgacagcc cttcgccatt cctgtagagg gaagcggcga
    15841 gggctatttc ttattacaga tatttatttc agaacagttt tcagtgcttc accgatatcc
    15901 gccaggctgc gaacggtttt cacgcctgcg gcttccagag cagcgaattt ctcatccgca
    15961 gtccctttcc caccagcaat gatcgcaccc gcgtggccca tacgtttgcc tttcggcgca
    16021 gtcacaccag cgatgtaacc tacaactggc ttggtaacgt gctctttgat gtacgctgcc
    16081 gcttcttctt cagcgctacc gccgatctca ccgatcatca cgatcgcttc ggtctgcgga
    16141 tctttttcga acatttcgag gatgtcgata aagttagagc ctgggatcgg gtcaccgccg
    16201 ataccaacac aggtcgactg accgaaaccg taatccgtgg tctgtttaac cgcttcatag
    16261 gtcagtgtac cggaacggga aacgataccc actttacccg gtttgtgaat gtgacccggc
    16321 tggataccga ttttgcattc acccggagtg ataacgcccg ggcagttcgg gccgatcata
    16381 cgaacgccgg cttcgtccag tttcactttc acagtcagca tatccagcgt cgggatgcct
    16441 tcagtgatgg tgataatcag tttgatgcct gcatcaatag cttccagaat ggagtctttg
    16501 cagaacggtg ctggtacgta gataacagaa gcggtagcgc cagtagcagc aacagcttca
    16561 cgcacggtgt tgaacaccgg caggccgagg tgggtagtac cgcctttacc tggggttacg
    16621 ccgccaacca ttttagtgcc gtatgcaatg gcctgttctg agtggaaagt cccctggcta
    16681 ccagtaaagc cctggcagat aaccttggtg tttttatcaa ttaaaatgga cattatttcc
    16741 cctccactgc ggcaacaacc tgctgagctg catccgtcag accttttgct gcaataatat
    16801 tcaggccgct gtcagccagt ttcttcgcgc cgagttcggc gttgttacct tccagacgta
    16861 ccacgaccgg tacgttaaca cccacttctg ctaccgcgcc gatgataccg tcagcgatca
    16921 ggtcgcaacg aacgataccg ccgaagatgt taaccagaac ggctttcact ttgtcgtcag
    16981 agaggatgat tttgaacgct tcggttacac gttctttggt tgcgccgccg ccaacgtcaa
    17041 ggaagttagc cggttcgccg ccgtgcagtt taacgatgtc catcgtaccc atcgccagac
    17101 ctgcgccgtt aaccatacaa ccgatgttac cgtccagcgc aacgtagttc agttcccact
    17161 gtgcagcctg tgcttcacgc ggatcttcct gcgactggtc acgcatttcg cgcagatcag
    17221 gctggcggaa cagtgcgttg ccgtcagcgc ccagtttgcc gtcgaggcaa atcagatcgc
    17281 cctgtttggt gatgaccagc gggttgattt cgatcaacgc caggtcgcgc tccaggaaaa
    17341 tggtcgccag gcccatgaag attttggtga actgctgaac cagtttacct tccagaccca
    17401 gtttgaacgc cagctcgcgt ccctgatacg gcatcgggcc agtcagcgga tcaagcgcaa
    17461 ctttgtggat caggtgcgga gtttcttccg ccactttttc gatttccacg ccgccttcgg
    17521 tggaggccat aaagaccaca cgacgggaac tacggtctac aacggcaccg agatacagct
    17581 ctttagcgat atcggtcgct gcttcaacca gaatctggtt aaccggttgg ccattggcat
    17641 ctgtttgata cgttaccaga cgcttgccga gccagttttc tgcaaaagca cggatgtctt
    17701 ctttgctgtt tacaactttc acaccgcccg ctttaccgcg gccaccagcg tgaacctgac
    17761 atttcactac ccacgggccg gcaccgattt ttgaagcggc ttcttctgct tcgcgcggag
    17821 tagtacaggc ataacccacc ggtgctggta agccatagcg ggcaaaaagt tgttttgcct
    17881 gatattcatg taagttcatg tgttctgtcc atccttcagt aatcgttatc ttttaaaccg
    17941 tagaccgaca ccctatccac gtcggtctac agtgcaggtg aagcttaaac tactacacgt
    18001 ccagcagcag acgcgtcgga tcttccagca actctttgat cgttaccagg aagcccacgg
    18061 attcgcgacc atcgatcaga cggtgatcgt aggacagcgc caggtacatc atcggcagga
    18121 tctcaacctg accattcacc gccatcggac gatctttgat agcgtgcata cccagaattg
    18181 cgctctgcgg cgggttgatg atcggcgtag acatcaggga accgaacaca ccaccgttgg
    18241 tgatggtgaa gttaccaccg gtcagatctt caaccgtcag cttgccgtca cggcctttaa
    18301 ctgccagctc tttgattttc ttctcgatgt ctgccatgcc gagggtatcg acatcacgca
    18361 gtaccggcgt caccaggccg cgcggcgtgg aaaccgccat gctgacgtcg aaatagttgt
    18421 ggtaaaccac gtcatcaccg tcgatagaag cattcacttc cgggtaacgt ttcagggctt
    18481 caaccaccgc tttcacgtag aaggacataa agcccagacg gatgccgtgg cgtttttcaa
    18541 acgcttcacc gtactgctta cgcagatcca taatcggctt catgttgact tcgttgaacg
    18601 tggtcagcat ggcggtggag tttttcgctt ccagcagacg ctctgccaca cgcttacgca
    18661 ggcgagtcat cgggacacgt ttttcactac gtgcagccag agccggttgc gccgccggag
    18721 cagccgctgc cggagcagac tctttcgccg gggctttcgc cagatgtttt tccacatctt
    18781 cacgagtcag acgaccaccc acaccggtgc ctttaatggc gctggcgtcg agattgtgtt
    18841 cagccagcag gcgacggatc gccgggctta acgcatcgtt gttttgctct tccagagacg
    18901 cctgctggcg ttgcgccgga gtggacgctt tctcttcaga tttggcgctg gtttctttac
    18961 cggcgctgtt gccttcacgc aggcgaccaa ggatctgacg agacgttacc gttgtacctt
    19021 catcttccag aaccgcatcc agaatgccgt ctgctgatgc cggtacttcc agtaccactt
    19081 tgtcagtttc gatttctacc agcacttcat cacgtacgac tgcgtcgccg ggttttttat
    19141 gccaggttgc gacggtggca tcggctacgg attcaggcag gtcagggacc agaatatcta
    19201 cgctactcat tgtgtatcct ttatttattc gacgttcagc gcgtcattaa ccagatcttg
    19261 ttgctgtttc tggtgaacgg acatataccc taccgccgga gaggcggagg ccgtgcggcc
    19321 tgcataacgc agagaagccc caaacggaat cacttcacgg aaatgatgct ggctgcagta
    19381 ccatgcgccc tggttgagcg gctcttcctg gcaccagaca aaatccttga cgtgagcaaa
    19441 ctgctgcaac acttcctgca tcgctttatg cgggaacggg tagagttgct cgatacgcac
    19501 aatggcgaca tcgtgttgat tgttcttacg acgctgttcc agcaggtcgt aataaacctt
    19561 accagaacac atcactacgc gcttcacgcc cttcggatca agctcgtcga tttcaccgat
    19621 ggctggcagg aaggtgccgt tcgccagttc ttcgaggctg gaaaccgcca gcggatgacg
    19681 cagcagggat ttcggcgaca tcactaccag cggacgacgc atcccgcgca gcgcctgacg
    19741 acgcagcatg tggtaaacct gtgccggggt agacggaacg caaacctgca tgttttgctc
    19801 agcacaaagt tgcagataac gttccagacg cgcggaggag tgctccggcc cctgcccttc
    19861 gtaaccgtgc ggcagcaaca tcaccagacc acacatccgg ccccatttct gttcgccaga
    19921 agagatgaac tggtcgataa ccacctgcgc accgttggcg aagtcaccga actgcgcttc
    19981 ccagatggtc agagtgcgtg gttctgcggt ggcataaccg tattcaaacg ccagcactgc
    20041 ttcttcagac agtacggagt cccagacgcg gaacgcgccc tgcccgttat ggatgtgttg
    20101 caacggcgtg taagtggaac cgttagactg gttgtggatc accgcgtggc ggtggaagaa
    20161 ggtaccgcga ccggagtctt cacccgacag gcgaaccgga atgccttcgt caaccagcgt
    20221 ggcgtaagcg aggttttccg caccgcccca gtcgaacagt ttctcaccgg cagccatcgc
    20281 ctggcgatcg ccataaatct tggcaacgcg agactgcatt tcaactgctt ccggcaccgt
    20341 gctgatgcgt ttcgccagct cctgcaggcg cttcatctca actttgttcg ggtactcttc
    20401 gtcccattcg tggttgaggt acggcgacca ggtgaaagag tgcatgttca tcggacgcca
    20461 ctctgccact acgcaatcgc cagcatccag cgcatcgcgg tacaggttaa ccatctcggt
    20521 ggcatcttcc agcgtcgcca ctttttcctg ctccagcttg tcagcgtaga ttttgcgcgg
    20581 tgtcggatgt tttttgattt tctgatacat cagcggctgg gttgcgctcg gctcgtcggc
    20641 ttcgttgtgg ccgtgacggc ggtagcacac caggtcgatg aagacatcac gtttaaaggt
    20701 gttacggaaa tcgagcgcca gacgggtcac aaaggcaacg gcttccggat catccgcgtt
    20761 aacgtggaaa atcggtgcct gaaccatctt accgatatca gtacagtacg gtgtagaacg
    20821 agcatccagt ggattagagg tggtgaaacc aacctggttg ttgataacga tacgtaccgt
    20881 accgccaact tcataaccac gcgctttcga catgttcaga gtttcctgaa ccacgccctg
    20941 cccggtcact gcggcgtcac cgtggatggt gattggcagc actttgttgc tgctcggctc
    21001 atcaagtctg tccagacggg cacgaacaga accgataact accgggctta caatctcaag
    21061 gtgagacggg ttaaacgcca gcgccaggtg caccaggccg ccatcggtct ggaagtcaga
    21121 cgagaagccc atgtggtatt tcacgtcacc cgtgccgagg tgttctttat gtttaccggc
    21181 aaactcgtcg aacaagtctt gcggtttttt acccagcacg ttcaccagca cgttcagacg
    21241 accacggtgc gccatcccaa gaaccacttc gcgggtgccg ctgttgccag cgtggcggat
    21301 catctcttta agcatcggga ttaacgcgtc accgccttcc agcgagaagc gttttgcgcc
    21361 agggaatttt gcgccgaggt aacgttcaag gccttcagcg gcggtcagtt cgcttaagaa
    21421 gcgttttttc tcttcgctat tgaaagtcgc gcgaccagac tcgatacgct gttggatcca
    21481 gcgtttttct tcggtgctgg taatgtgcat atactcggca ccaatcgggc cacagtaggt
    21541 ttgcttgagg gcttccagca gctcgccgag tttcatgttt tctttgccgc tggcaaatga
    21601 accgacgttg aaggtctcct ggaagtctgc ttcggtcaga tcgtggaaag acggatccag
    21661 atcggccact ttatcttgct gccacagtcc cagcggatcg agattcgcat gctggtgacc
    21721 acggaagcgg tatgcgttaa tgagctgcag gactttaacc tgcttcacat tggtgtcagg
    21781 gtcggagatc gttgaagagt aacgtgaagc gtctttcgcc aggcggcgga aatattcacg
    21841 cgtttgagag tggaattgat ccggtttgac tcccgtacca ggtaactgct ggaacgtcga
    21901 acgccagtta gcgtcaaccg agtcaggatc ggttaagaag tcttcataga gctgttctat
    21961 ccagctctgg tttgcgccag agaggtaaga agagtccaac caggctttca aagcgctgtt
    22021 ctgcatcgtg atcccttaag catctttttt atgcttactt cgccgtggat actaccacgc
    22081 acagtgatgt gcgtgccggg gttcacttgc gagctcttgt ctatgtagtg gctcgcgaag
    22141 gaacctttaa aaactgtctt agtgtcaggg gcagttttta aagatttcct gcatttcttt
    22201 gttacgcctg atgcgctgcg cttatcaggc ctacgggtaa tgacttgtag gccggataag
    22261 gcgttcacgc cgcatccggc actggttgcc tgatgcgacg cttgcgcgtc ttatcaggcc
    22321 tacggtttac gcattacgtt gcaacaacat cgacttaata tggccgatgg cgcgcgtcgg
    22381 gttcagcccc ttcggacata cactgacgca gttcatgatg ctgtgacagc ggaatacgct
    22441 gaatgcatcg ctcaaaccgt cgaggcggct gtcagtctcg gtatcacggc tatcgatcag
    22501 gaaacgatat gccgctaaca agcctgccgg gccgataaac ttatcgggat tccaccagaa
    22561 agacggacaa gaggttgaac aacatgcgca gagaatacat tcatacagcc cgtcgagttt
    22621 ttcgcgctgc tctggcatct gtaaatgctc gcgagctggc ggattttgtc cattattcaa
    22681 caggtaaggc ttaattttct catattgcgc atagaattgt cccatgtcta ccaccaaatc
    22741 gcggatcacc ggtaaacctg gcagcgggcg aatcacaatc ttcttgcccg gctggttgag
    22801 tgccgaaatc ggggtaatac aggccagacc attcttgccg ttcatgttca gaccgtcgga
    22861 accgcacaca ccttcacggc aggagcggcg gaacgacagg ctgggatctt tctctttcag
    22921 ctggataagc gcatccagca gcatcatgtc gcgaccttct tccgcttcca gggtgtaatc
    22981 ctgcatacgc ggagcatcat caacatccgg gttatagcga taaattgaaa actcgagtct
    23041 cattttcctg tctccgcatt agtaagtacg aatcttcggc gggaatgccg ggcgcagttt
    23101 cggttccatg ttgacgcttc ggcgcgtcat ggattccgac tctggcagat acagggagtg
    23161 gcacagccag ttttcatcat cacgatccgg gaagtcgaag cggctatgtg cgccacggct
    23221 ttcggtacgg aagttggcag aaacagccgt tgcatacgcc gtttccatca ggttatccag
    23281 ttccaggcac tcaacgcgct gggtgttgaa ctcgctggaa gtgtcatcca gacgggcatt
    23341 tttcagacgc tcgcggatca ctttcaactg ctcaagccct ttcgccatcg catcaccttc
    23401 acggaagacc gagaagttat gctgcataca ttcttgcagc gctttacgaa tcgccaccgg
    23461 atcttcaccg ttacgattat tgttccagcg gttcagacga tccagagatg cttcaacatc
    23521 agactcgctg gcatcgcgca atgcgccctg ttcggcgata gactcttgca gatgcagacc
    23581 tgccgcacga ccaaagacca ccaggtccag cagcgagttg ccgcccagac ggttagcgcc
    23641 gtgtaccgat acacaagcga tttcaccaac ggcaaacagc cccggaacaa ccacatcttc
    23701 gcctttctcg ttcaccgtca gcgcctgacc ggtcactttg gtcggaatac cgcccatcat
    23761 gtagtgacag gttgggataa ccggaatcgg ctctttcacc ggatcgacgt gagcaaaggt
    23821 acgggaaagc tccaggatac ccggcagacg ggattcgaga acttctttac ccaggtgatc
    23881 gagtttcagt ttcgcgtgtg gcccccacgg accatcacag ccgcgacctt cacggatttc
    23941 aatcatgatg gaacgcgcca ccacatcacg gcccgccagg tctttggcgt tcggcgcata
    24001 acgctccata aagcgttcgc catgtttgtt cagcagataa ccgccttcac cacggcaacc
    24061 ttcggtgacc agtacgcccg caccggcaat gccggtcggg tggaattgcc acatttccat
    24121 atcctgcacc ggtacgcctg cgcggatagc catgccgaca ccgtcgccag tgttaatgtg
    24181 ggcgttagtg gtggactgat aaatacgccc tgccccgcca gtcgccagca cggtagcgcg
    24241 ggctttgaaa taaaccactt caccggtttc gatgcacagt gcggtacaac ccaccaccgc
    24301 gccatcctgg tttttcacca gatccagcgc ataccactcg gagaaaatgg tggtgtggtt
    24361 tttcaggttc tgctgataaa gcgtgtgcaa cagtgcgtga ccggtacggt cagccgccgc
    24421 cgcagtgcgt gccgcctgct cgccgccgaa gtttttcgac tgaccgccaa acggacgttg
    24481 atagatacga ccatcatcaa gacgcgagaa cggcaggccc atgtgttcga gttccagaat
    24541 cgcttccggc ccggttttac acatatattc aatcgcgtcc tggtcaccga tatagtccga
    24601 cccttttacg gtgtcgtaca tatgccattc ccagttatct tcatgggtat tacccagcgc
    24661 aacggtaatg ccgccttgcg cagaaacggt atgggaacgg gtcgggaaga ctttagagag
    24721 cagcgcacag gtctggccgc tctgggaaat ttgcagcgcc gcgcgcatac ctgcgccacc
    24781 ggcaccaatc acaactgcat caaattctct gactggcaat ttcatcacac accccacacc
    24841 acaacgaatc cataaatcac gtaaaccacc agtgcaacga caatcaccag ttgcagcatc
    24901 aggcgcaatg ccagcggttt aacgtagtcg gtcaacacct gccacatgcc gatccaggca
    24961 tgaatcaaga tagaaaacag cgccagcagg gtgaacactt tggtgaacgc agaggcgaag
    25021 aaaccgatcc agacttcata tgtcagttcg ccactggtgg cgaaaaaacc gaccatataa
    25081 atgatgtaga gcgtcaggac gatagcggta gcgcgaacga ggatgaaatc atgtacgcca
    25141 ttgcgtccta atgcggaggc gttgcttacc atacgaggac tcctgcgaga agtgaaagca
    25201 cgacagtaat aacaaaggag attttggcgg agcgtttacc cgcttcgaat gtttcttcca
    25261 gatagccaaa atccatcatc atgtggcgaa tacctacgac gacgtgatac gccagagcgg
    25321 taaggatgcc ccacatgata aatttgacga agaagctgcc cataatcgcg gaagcttgct
    25381 cgaaaccttc aggggaagag aggctggtac ccagaagcca cagcaggatg cccactgcaa
    25441 caaaggtgat cacaccggaa acgcgatgga gaatggacgc tatcgccgtg atggggaacc
    25501 ggatggtctg taggtccaga ttaacaggtc tttgtttttt cacatttctt atcatgaata
    25561 acgcccacat gctgttctta ttattccctg gggactacgg gcacagaggt taactttctg
    25621 ttacctggag acgtcgggat ttccttcctc cggtctgctt gcgggtcaga cagcgtcctt
    25681 tctataactg cgcgtcatgc aaaacactgc ttccagatgc gaaaacgaca cgttacaacg
    25741 ctgggtggct cgggattgca gggtgttccg gagacctggc ggcagtatag gctgttcaca
    25801 aaatcattac aattaaccta catatagttt gtcgggtttt atcctgaaca gtgatccagg
    25861 tcacgataac aacatttatt taatttttaa tcatctaatt tgacaatcat tcaacaaagt
    25921 tgttacaaac attgccagga aaagcatata atgcgtaaaa gttatgaagt cggtatttca
    25981 cctaagatta acttatgtaa cagtgtggaa gtattgacca attcattcgg gacagttatt
    26041 agtggtagac aagtttaata attcggattg ctaagtactt gattcgccat ttattcgtca
    26101 tcaatggatc ctttacctgc aagcgcccag agctctgtac ccaggttttc ccctctttca
    26161 cagagcggcg agccaaataa caaacgggta aagccaggtt gatgtgcgaa ggcaaattta
    26221 agttccggca gtcttacgca ataaggcgct aaggagacct taaatggctg atacaaaagc
    26281 aaaactcacc ctcaacgggg acacagctgt tgaactggat gtgctgaaag gcacgctggg
    26341 tcaagatgtt attgatatcc gtactctcgg ttcaaaaggt gtgttcacct ttgacccagg
    26401 cttcacttca accgcatcct gcgaatctaa aattactttt attgatggtg atgaaggtat
    26461 tttgctgcac cgcggtttcc cgatcgatca gctggcgacc gattctaact acctggaagt
    26521 ttgttacatc ctgctgaatg gtgaaaaacc gactcaggaa cagtatgacg aatttaaaac
    26581 tacggtgacc cgtcatacca tgatccacga gcagattacc cgtctgttcc atgctttccg
    26641 tcgcgactcg catccaatgg cagtcatgtg tggtattacc ggcgcgctgg cggcgttcta
    26701 tcacgactcg ctggatgtta acaatcctcg tcaccgtgaa attgccgcgt tccgcctgct
    26761 gtcgaaaatg ccgaccatgg ccgcgatgtg ttacaagtat tccattggtc agccatttgt
    26821 ttatccgcgc aacgatctct cctatgccgg taacttcctg aatatgatgt tctccacgcc
    26881 gtgcgaaccg tatgaagtta atccgattct ggaacgtgct atggaccgta ttctgatcct
    26941 gcacgctgac catgaacaga acgcctctac ctccaccgtg cgtaccgctg gctcttcggg
    27001 tgcgaacccg tttgcctgta tcgcagcagg tattgcttca ctgtggggac ctgcgcacgg
    27061 tggtgctaac gaagcggcgc tgaaaatgct ggaagaaatt agctccgtta aacacattcc
    27121 ggaatttgtt cgtcgtgcga aagataaaaa tgattctttc cgcctgatgg gcttcggtca
    27181 ccgcgtgtac aaaaattacg acccgcgcgc caccgtaatg cgtgaaacct gccatgaagt
    27241 tctgaaagag ctgggcacca aagatgacct gctggaagtg gctatggagc tggaaaacat
    27301 cgcgctgaac gacccgtact ttatcgagaa gaaactgtac ccgaacgtcg atttctactc
    27361 tggtatcatc ctgaaagcga tgggtattcc gtcttccatg ttcaccgtca ttttcgcaat
    27421 ggcacgtacc gttggctgga tcgcccactg gagcgaaatg cacagtgacg gtatgaagat
    27481 tgcccgtccg cgtcagctgt atacaggata tgaaaaacgc gactttaaaa gcgatatcaa
    27541 gcgttaatgg ttgattgcta agttgtaaat attttaaccc gccgttcaaa tggcgggtta
    27601 atttttatat gcccaaacac aaaaaattgt aaaaataaaa tccactatcg gacattcata
    27661 tattctaaaa aaaatatatc ggtataaatt ataaacaaat cgatgatttt taatcatcta
    27721 tcttcctctc agttacatga
//
LOCUS       DAZPLI010000042        27641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZPLI010000042 DAZPLI010000000
VERSION     DAZPLI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.042"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..643)
                     /locus_tag="ACHUU3_001728"
     CDS             complement(<1..643)
                     /locus_tag="ACHUU3_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR2081816.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDL"
     gene            complement(743..1654)
                     /gene="gsiD"
                     /locus_tag="ACHUU3_001729"
     CDS             complement(743..1654)
                     /gene="gsiD"
                     /locus_tag="ACHUU3_001729"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR2081817.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1657..2577)
                     /gene="gsiC"
                     /locus_tag="ACHUU3_001730"
     CDS             complement(1657..2577)
                     /gene="gsiC"
                     /locus_tag="ACHUU3_001730"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR2081818.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2595..4133)
                     /gene="gsiB"
                     /locus_tag="ACHUU3_001731"
     CDS             complement(2595..4133)
                     /gene="gsiB"
                     /locus_tag="ACHUU3_001731"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR2081819.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4153..6024)
                     /gene="gsiA"
                     /locus_tag="ACHUU3_001732"
     CDS             complement(4153..6024)
                     /gene="gsiA"
                     /locus_tag="ACHUU3_001732"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR2081820.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6011..6976)
                     /gene="iaaA"
                     /locus_tag="ACHUU3_001733"
     CDS             complement(6011..6976)
                     /gene="iaaA"
                     /locus_tag="ACHUU3_001733"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR2081821.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7180..8415
                     /gene="moeA"
                     /locus_tag="ACHUU3_001734"
     CDS             7180..8415
                     /gene="moeA"
                     /locus_tag="ACHUU3_001734"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR2081822.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8415..9164
                     /gene="moeB"
                     /locus_tag="ACHUU3_001735"
     CDS             8415..9164
                     /gene="moeB"
                     /locus_tag="ACHUU3_001735"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR2081823.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9343..10005)
                     /gene="fsa"
                     /locus_tag="ACHUU3_001736"
     CDS             complement(9343..10005)
                     /gene="fsa"
                     /locus_tag="ACHUU3_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR2081824.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10136..11035
                     /locus_tag="ACHUU3_001737"
     CDS             10136..11035
                     /locus_tag="ACHUU3_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR2081825.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11041..13473
                     /locus_tag="ACHUU3_001738"
     CDS             11041..13473
                     /locus_tag="ACHUU3_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR2081826.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13619..14434
                     /gene="ybiV"
                     /locus_tag="ACHUU3_001739"
     CDS             13619..14434
                     /gene="ybiV"
                     /locus_tag="ACHUU3_001739"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR2081827.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALNGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14586..15851
                     /locus_tag="ACHUU3_001740"
     CDS             14586..15851
                     /locus_tag="ACHUU3_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR2081828.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15818..16978)
                     /locus_tag="ACHUU3_001741"
     CDS             complement(15818..16978)
                     /locus_tag="ACHUU3_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR2081829.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNFSSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            17216..18517
                     /locus_tag="ACHUU3_001742"
     CDS             17216..18517
                     /locus_tag="ACHUU3_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2081830.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWVHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18532..20895
                     /locus_tag="ACHUU3_001743"
     CDS             18532..20895
                     /locus_tag="ACHUU3_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HFR2081831.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLDFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(21075..22670)
                     /locus_tag="ACHUU3_001744"
     CDS             complement(21075..22670)
                     /locus_tag="ACHUU3_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR2081832.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22889..23809
                     /gene="ldtB"
                     /locus_tag="ACHUU3_001745"
     CDS             22889..23809
                     /gene="ldtB"
                     /locus_tag="ACHUU3_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR2081833.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23868..24986)
                     /locus_tag="ACHUU3_001746"
     CDS             complement(23868..24986)
                     /locus_tag="ACHUU3_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR2081834.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24983..25450)
                     /gene="mntR"
                     /locus_tag="ACHUU3_001747"
     CDS             complement(24983..25450)
                     /gene="mntR"
                     /locus_tag="ACHUU3_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR2081835.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25636..25764
                     /gene="mntS"
                     /locus_tag="ACHUU3_001748"
     CDS             25636..25764
                     /gene="mntS"
                     /locus_tag="ACHUU3_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR2081836.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            26036..27619
                     /gene="opgE"
                     /locus_tag="ACHUU3_001749"
     CDS             26036..27619
                     /gene="opgE"
                     /locus_tag="ACHUU3_001749"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR2081837.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
BASE COUNT         6733 a         7634 c         6931 g         6343 t
ORIGIN      
        1 acaaatcgtg atctttgccg ttgatacgta gcgtaaaaga gatacatttt ttacccggac
       61 gatcaaagca attgtatgcc ggattccagc tgttgttaga gtcatctgaa gccaccgacc
      121 acggtcgttt cgatgggtta aaatcggcaa aaataacccg ccctgactcg tcgacaagcg
      181 gtccatttac ttgttcctga tgataaacaa cgattttatc attcgccctc cgcgaaaaga
      241 gataatattt attatcatca agtaccagtc ccaccgagtc taaaaatggc atcatttcca
      301 ttttcagaat cacggcgcgt tgaatctcct ctgtattttt taataaatac agtgagtcct
      361 ttaatgtcag ggccgaacgt tcaatatctt taaagaaata gccaacgttt ttctgaataa
      421 gttcaaagcg gctatcggca cgttttaccg cgcgttccga taccagttga tagttaatga
      481 tgaaaaaaag accaataaat gacagtgctg ccaataaaaa taaaattatc agccttatct
      541 ttatcttttc gtataaactc agcatgttaa tacatttaac aaaataacta tctgaacgct
      601 gtgccgctgc aaaaatagtc ccttgctgcg gctttttaac caaattaccc gattctacaa
      661 aatcagcaga aagttacaac acttgcttag ggcaataatt tcctgtgagg tgattaccct
      721 ttcaagcaat attcaaacgt aactatcctt taattttcgg atccagcgca tcgcgtaaac
      781 catcgcccaa caaattgaac gccagtacgg tcagaaaaat agccagggcc ggaaaaacag
      841 cgacatgcgg cgcgataacc atatccgctc gtgcctcatt gagcattgct ccccactctg
      901 gtgtcggcgg ctgcgcaccg aggccgagaa atgacaggct ggcggcagag ataatcgagg
      961 tgccaatgcg catggtgaaa aacaccacga tagacgagac ggtcccaggc aagatatgac
     1021 gcaataaaat ggtcatatcg ctggcaccaa tactgcgtgc tgactcaata aaggtttgct
     1081 gtttcaacac cagcgtgttg ccgcgcacca gtcgggcaaa cgcggggatg gaaaaaatgg
     1141 cgactgcgat aatcacgtta gcaatgccgc ttcccaacac cgcaacaaca gcgatcgcca
     1201 gtaaaatacc cgggaaggca aacagcacat cgcaaatgcg catgatcagc cgatcccacc
     1261 agccttcata atatccagcc aacaagccca gcaacgtccc gatcgccgca ccgataaaca
     1321 cggcaaacac gcccgccgcc agcgagattt gcgcaccaac caggacacgg ctgaaaatgt
     1381 cgcgccccag tgaatcgacg ccaaaccagt gctgcaaaga aggtccgtta ttcagattgt
     1441 cataatcaaa ataattttcg gcgtcatagg gagcgatcca gcgtgcaaaa atggccacca
     1501 caatcaataa aataacgaat aatgcggcgg tcatcgccat atgctgacgg cgaaatcgtc
     1561 gccagaattc atgccacggt gtacgcacct ggtcaggttt gaccagtggc atggcgttta
     1621 acaccgcctg acgtcgccag ttaaatagtc gcatccttac ttgtacctga tagccgggtt
     1681 aatggcggcg taaagcacat ccaccactaa gttgataaga ataaattcca gcgagaaaag
     1741 cagaatttcc gcctgaatca ccgggtaatc gcgcatttct acggagtcaa cgagtaagcg
     1801 tccaagtccg ggccagttga aaactttctc caccacgata gaaccaccga gcaaaaagcc
     1861 aaactgtaag cccatcatgg tcactaccgg gatcatcgcg ttacgtagcc cgtgtttgag
     1921 gacaacccag gtttcgctca cccctttcgc cctcgcggta cgcatataat cttcgcttaa
     1981 aacatcgaca aacgacgcgc gggtaaagcg cgccatcacg gctgccactg ccgcgccgag
     2041 cgtcagggag ggtaaaatgt agtgctgcca actgtctgct cccacggtag gcagccagcc
     2101 cagttcaacg gagaatacct gaattaaaag catccccagc gcaaatgccg gaaacgagat
     2161 ccccgacacc gcaatggtca tactcaatcg atccggccaa cgattacgcc agacggcggc
     2221 gataattccc gccgccatac caaatatcac cgcccagacc atactggtta tggtcagcca
     2281 cagcgttggc ataaagcggc tggcaatctc atcggcaacc ggacgacgcg acaccatcga
     2341 caggccaaaa tccccctgca cagcattgct gatatagtgc cagaactggt gatacagcgg
     2401 ctgatccaac cccagctgct gacgcaccag ttctataacc tgcgcatcag cttcgagccc
     2461 ggcaatcaat cgcgccggat cgccgggcag catatgcaca aataaaaaca ccagcaccga
     2521 gacgataaac agcgtcggaa tcaaccccag taagcgtttg ataacgtaat taagcattcc
     2581 actccctgcg ttgcttattg caaatccgcg tcttcaaagc tgaagccggt gtctggcatg
     2641 atccaaaaac cggtcaggtt tttactgtgt gccgacacca gtttttctac caccagcggg
     2701 atccacggcg actcttgcca gatgatatcc tgcgccgcct tatacaagcg ggtcttttcc
     2761 gccggatcat tagttttcag tgcctgagcc aggaagtcgt ccacctgttt attgctgtaa
     2821 aatgcggtat taaacagcgt cggtggccag ttttgcgagg cgaacagtgg cgatagcgcc
     2881 cagtccgctt cgccggttga agccgaccag ccagtgtaga acatccgcac gccgctctct
     2941 ttttgccctt taccttcaac ttctgccgcc cgctgtccgg catccatcgc ggtcacctgg
     3001 gctttaatcc cgacctgcgc taactgctgc tgggtaaatt gcagcacttt ctgcgcggtg
     3061 ctgtggttat gtgacgacca cagcgtggta ctgaaaccgt tgggatatcc cgcctctttc
     3121 agtaattcgc gcgctttcac tggatcgtaa ggccacggtt tataactttg cgcgtaggcg
     3181 atacttggcg gtaccacacc agtagctggc gttgcatagc ccgcaaaggc aactttcacc
     3241 agcgccggac ggttaatggc gtaattcagc gcctcacgga ccttcgggtt atcgaacggc
     3301 ttttgcgtca cgttcatact gatataacgc tgcataattg acggactggc catcaactcg
     3361 atatttttgt ttttctccag cagtgtggcc tgctcgtagg gaatggggaa agcaaactgc
     3421 gcttcaccgg tttgcagcat tgccgcgcgg gtgttgttat ccgccaccgg acgccaggtt
     3481 atgctgtcca gtttgggcaa tcctggctgc cagtaacccg cgaatttttt caccttcaca
     3541 aaatcggtct gattccaggt atccagttca tacggtccgg ttcccaccgg atgaaaacca
     3601 atctccttgc catatttttc cagcgctgcc ggtgaaatca tcgcggtcgc cggatgggca
     3661 agaatattaa taaacgctga gaacggctgt ttgagggtaa tctttaccgt tgtcggatcg
     3721 atcgcctccg ttttggcaat attcttatac aggttatagc gtttaagatg attcgccgga
     3781 tcgctggccc ggtccagatt cgcttttacc gccactgcgt tgaaatcggt gccatcctgg
     3841 aatttaatgc cttcccgcaa tttcacggtg taagtaatgc cgtcatcgga aacggtataa
     3901 ctctccgcca gcacgttttt cagtttcatc tctttatcca gaccgaacag cccctggtaa
     3961 aacgatttcg ctacggcctg agataacgtg tcattcgcgt catacggatc gagcgtggtg
     4021 aaattcgatc ctaccgccac caccacatct ttggcagcga atgcacaaga tgccatcaac
     4081 gctgtcgcaa tgcccagcgc cactaaccca ctacggtgta cagctcttgc cattttattc
     4141 tccgcctgaa tgttatctac gcatgaatgc gtattctgat tgtggttgtg cgacgtaatg
     4201 ccccggcccg acgcattgca acgagacggc tgccacctct tcgccacgca gatgaatatt
     4261 gctgggaaga tcgtccgaca gcagtacacg ctgcggtcgt tgtcgggacg gttcagcgac
     4321 cggaactgcc gccagtaatt tacgcgtata aggatgctgc gggttttcga agaccgcgcg
     4381 ccgtggacca atttcaacaa tttgcccgag atacatcacc gccacacgat gactaatccg
     4441 ctctaccacg gccatatcgt gggagataaa cagatacgca atgccgaaat cacgctggag
     4501 atcgagcaac aagttgataa tctgcccgcg aatagaaaca tccagcgccg aaacggcttc
     4561 gtcggcaatg atcacttttg gattcaatgc caacgcgcga gcaatgcaga tgcgctggcg
     4621 ctgaccgccg gaaaactcat gcgggtaacg ccaggcatgt tcaggtaaca ggcccacgcg
     4681 ctccagcaac cacgcaacgc gtgcagccgc gtctttacct ggcaataaac cgtgtacacg
     4741 cagcggttcg ataatcgaat caccgatggt ctgacgtggg tccagcgaag cgtaagggtc
     4801 ctgaaaaata aactgaatat cccggcgcaa tgcctgaagt ttgccgggtg acaaggtatc
     4861 gattctctga ccgttaaaga taatttcgcc gccctgcgat tcgaccaggc gcagcaacgc
     4921 ccgcccggta gtggatttac cgctgccaga ctcgcccacc agcgatagcg tttcgccagg
     4981 ccagagatca aaactgactt tctcaacggc atgcacttcc cgcgttacgc gattcaacaa
     5041 accgctgcgc aaagggaaac gggtgacaag attacgcact cgtaaaacag gttcgccatc
     5101 caccaccgtt ttctgctcga tggggggggc ctgtttcgct ggatgttcaa gcgatatcaa
     5161 cgggaaacgt cggggataat ccaacccttt catcgcacca agttgcggaa cagcagctaa
     5221 cagcgcacgg gtgtaaggat gttgcggtgc atgaaaaatc tgttcgacgg tacccgtttc
     5281 caccgcctcg ccctgataca tcaccagtac ccgatcggca atctctgcca ccacgcccat
     5341 atcgtgagtg ataaagataa cgcccatcga catctctttt tgcaatactt tgattaattg
     5401 caggatctgc gcctgaatag tgacatccag cgcggtggtt ggctcatcgg caatcagcac
     5461 cgccgggcgg catgacagcg ccatcgcaat catcactcgc tggcgcatcc cgccagagag
     5521 ttgatgcgga taacgtgaaa gaatggtttg tgcctcagga atgcgtacct gatccagcat
     5581 ccgcttcgcc tcgaccatcg cttcttcacg actggcgttc tgatgcagac gaattgattc
     5641 ggcaatctgt tcacccacag taaataccgg gttcagcgat gtcatcggct cctgaaatat
     5701 catcgccata tccgcaccgc gaacatggcg catttgtgca gcgctctgct cgctaagttc
     5761 aatcacttcg cgactgcgcc gctgcaacag cattttatcg cactgtacta aaccgcccgc
     5821 ctgttccaac aggcgcatca atgccaacgc agtcactgac ttaccggagc cggattcgcc
     5881 aacaattgcc agcgtctcac cgcgttgcag actaaaagag agattgcgga ccgcagctat
     5941 tttctgctgg tcctgcataa aggcaatatt cagattttca accgccagca cattaccggc
     6001 atcaagttca tcactgtgtg gcaacggtgt cccctttttc acggtagata ccggtggttg
     6061 gcgtatcgcc tgcgtagccc caggcgcgat acattccttc ggtgttaaac ggtagcgcga
     6121 cattcccttc atggtcgata gcgattaagc caccgctacc gccaagcgca gggagttttt
     6181 ccattactac ccgctcgcag gcttccgcga gacttaatcc gccgtaatcc attaacgcgg
     6241 cgatgtcata tgccgccagc gcgcggatga agacttcgcc cgtgccgata caagaaaccg
     6301 ccacactggc gttattggcg tagcatccgg cacccactaa gggactatcg ccaactcgtc
     6361 cgggtaattt attggtcatt ccgcctgtgg acgtggctgc cgccaaattg ccgtctaaat
     6421 ccaacgccac ggcccccacg gtgcccattt tctgtttttc atccagtggc gcaccgctat
     6481 ggtcgaggac ggttgcccct tccttgcgcg ctgccagtag ttgttcataa cgcaaaggcg
     6541 tggagaaaat ctccggcgag acgcgctcca tgccacgagc aaacgcaaaa ttttctgccc
     6601 cttcgccaat catcatcaca tgcgggcttt gctccatcac cagccgggcg gcaagaaccg
     6661 gattacgcag atgactaacg cccgccaccg caccggcttt cagggtgtta ccatccatca
     6721 cacaggcgtc cagttcatgg gtttcatcac gcgtaaagac agcgccaatt ccggcgttaa
     6781 acagtggaca ctcttccagc agacgcaccg cttccgtcac cacatccagc gcactttcgc
     6841 ccgcttccag cattttctgc ccggtttcaa caatggcaga caacgcctca atgtagcgta
     6901 attcctgttg cagactcatc tgcgcgcggc taattgcacc tgcgccacca tgaattgcaa
     6961 tgactgcttt gcccatagta ttcgtccatt aagggtggct cgccactttt gctataaata
     7021 tcagaatttt tatgaattac tcattcaatt tttgaatata gaaagatgga tatggcatgt
     7081 aaaggcagga gccaacgcta cgcccgaggt ggcatacagt ctgtggcgtt tttctgacat
     7141 aataggcaaa ttcgattttg cctccgcagg agtgttttca tggaatttac caccggattg
     7201 atgtcgctcg acaccgcgct taatgagatg ctttctcgcg tcaccccact gaccgcccag
     7261 gaaacgctgc cactggtaca gtgttttggt cgtattctgg cgagcgatgt cgtttcgccg
     7321 cttgatgttc cggggtttga taactccgca atggacggct acgcggtgcg tttagccgat
     7381 attgcctccg ggcaaccgct gcccgttgcc ggtaaatcct ttgccggtca gccgtatcat
     7441 ggtgaatggc ctgcgggcac ctgcattcgt attatgaccg gtgcgccggt gccggaaggc
     7501 tgcgaagcgg tggtgatgca ggagcagact gaacaaacgg acaatggcgt gcgttttact
     7561 gctgaagtgc gtagcgggca aaatattcgc cgtcgcggtg aagatatctc tgcaggtgcg
     7621 gttgttttcc cggcgggaac tcgcctgact accgcagagc tgccagtaat tgcttcactg
     7681 gggattgccg aagttccggt gattcgtaaa gtgcgtgtag cgcttttttc taccggtgat
     7741 gaactccagt tgcccggtca gccgctgggc gacggccaaa tctacgacac caaccgtctc
     7801 gccgtacacc tgatgttaga acagctgggt tgcgaggtaa tcaacttagg gattatccgc
     7861 gacgatcccc atgccctgcg cgccgcattt attgaagccg acagccaggc ggatgtagtg
     7921 atcagttccg gcggtgtttc agtgggtgaa gcggattaca ccaaaacgat tcttgaagag
     7981 ctgggggaga tcgccttctg gaagctggcg attaaaccag gtaaaccgtt cgcgttcggt
     8041 aaactcagca atagctggtt ctgcggcctg ccgggcaacc cggtttcagc gacgctgacc
     8101 ttctatcaac tggtacagcc tttgctggca aaactaagcg gcaacaccgc cagcggcctg
     8161 cccgcgcgcc agcgcgtacg cacagcgtcc cgcctgaaaa aaacgccagg acgtcttgat
     8221 ttccagcgcg gcgtgctgca acgcaacgcc gacggtgaac ttgaagtgac gaccaccgga
     8281 catcagggtt cacatatatt tagctccttt agtctcggca actgctttat cgtgctggaa
     8341 cgcgatcgcg gcaatgtgga cgtgggcgaa tgggtggaag tagaaccgtt taacgcgttg
     8401 ttcggaggcc tgtaatggcg gaactcagcg atcaggagat gctgcgctac aaccggcaaa
     8461 tcattctgcg cggttttgat tttgacggtc aggaggcgct aaaagattct cgtgtgctga
     8521 tcgtcggcct cggtggactc ggctgcgcag cttcgctgta tctggcaagc gccggtgtcg
     8581 gcaacctgac gctgctcgac ttcgacacgg tttcgctctc gaatctgcaa cgccagacac
     8641 tgcacagtga tgccacggtc gggcaaccga aggtggaatc cgcccgtgac gccctgacgc
     8701 ggatcaaccc acatatcgcg attacgccag tcaatgcact gctagatgac gcagaacttg
     8761 cagcattgat tgctgaacac gatctggtgc tcgactgtac ggataacgtt gcggtacgta
     8821 atcaactgaa cgcaggctgt tttgccgcga aggtgccgct ggtttccggc gcggcaattc
     8881 gtatggaagg gcaaatcacg gtcttcactt atcaggacgg cgaaccgtgc tatcgctgcc
     8941 ttagccgttt gtttggcgag aacgcattaa cctgcgtaga agcaggcgtg atggcaccgt
     9001 tgatcggcgt aattggttcg ttgcaggcaa tggaagcgat caaactgctg gcaggttatg
     9061 gcaaaccgac cagcgggaaa atcgtcatgt acgatgcgat gacctgtcag tttcgcgaaa
     9121 tgaaactgat gcgcaatccg gggtgtgagg tgtgcgggca gtaattgcca cctccaggcc
     9181 tgataagacg cgacaagcgt cgcatcaggc atttgctcca gacgccagat gcggcgtgaa
     9241 cgccttatct ggcctacggt tcgggcacag atttccaagc gcggcaggca tcggtgcact
     9301 ccgccacgtc atcagcaatt tatgaggatg cagaacgtcc ggttaaatcg acgttctgcc
     9361 aaacgctccc tgccagtcct gctcaaactt cgccacagcg gcttccaccg ccggatagct
     9421 aatcatctgt tgtgccacat ccagtggcag agtaattgat tcacatcctg ccagtaagca
     9481 gtccagcgcc tgacgcgggg ttttgaaact cgctgccagc actttcgcct gcggcgcatg
     9541 cattttcaat aactggtgta agtcggtcac agtctgaatg ccgctaccgc cctgagcatc
     9601 aatacgatta acgtaaggcg caacatattc cgcacctgcc agcgccgaca gcaacccttg
     9661 tgctgcgcca tataccgcgg ttcccagcgt tggaatccct tccgctttta acatcttaat
     9721 agctgccagc ccctcggcgg tcaccggaac tttcaccacg atatccgcaa taatagaacg
     9781 caatttacgt gcgtcattca ccatcccttc agcagtggta gccattacct gggcaaacag
     9841 acgcccctga ccgcccatcg cttcatgaag ttgcggaagc acaacatcca gcggtttttt
     9901 acccgcggcg ataatgcttg ggttagtggt cacacccgcc agcggaaaaa tacgtgacag
     9961 cgccttcacc gcaacaacgt ctgaagtatc cagatacagt tccataacca tcctcaaaat
    10021 gtactttttg tctgaatgta gactatcacg ggaaaactct ctgaacagtt gacgcacatc
    10081 aatctaactt tcattcgaaa gtattttaat gtttgaacga aacagagagg caactatgat
    10141 tttcaatatt cagcgctact cgacccatga tggccccggt atccgcacgg tcgtatttct
    10201 taaaggctgt tcgctgggct gccgctggtg tcagaacccg gaaagccgcg cccgcacgca
    10261 ggatctgctg tatgacgcac gactgtgtct ggaaggctgc gagctgtgcg ctaaggccgc
    10321 gccggaagtg attgagcgcg cgctgaatgg tttgcttatt catcgggaaa agttaacccc
    10381 ggagcatctg acggcgttaa ccgactgctg tccgacacag gcattaaccg tgtgtggtga
    10441 agtgaaaagc gttgaggaga tcatgacgac cgttctgcgc gataaaccgt tttacgatcg
    10501 cagcggcggc ggtttaacgc tttcgggtgg tgagcccttt atgcagccgg aaatggcgat
    10561 ggcgctactg caagccagcc acgaggcagg cattcatact gcggtagaaa cctgtctgca
    10621 tgtgccgtgg aaatatatcg ccccttctct gccctatatc gatctgtttc ttgccgattt
    10681 aaaacacgtt gccgacgcgc cgtttaaaca gtggaccgac ggtaacgccg ccagagtgct
    10741 ggataacctg aaaaaactcg ccgcagcggg caaaaaaatc attatccgcg tgccgctgat
    10801 tcagggcttt aatgccgacg aaacctctgt aaaagctatt accgattttg ccgccgacga
    10861 gctgcacgtt ggcgaaattc atttcctgcc ctaccacacg ctaggcatca acaaatatca
    10921 cttacttaat ctgccctatg acgccccgga aaaaccgctt gatgcgccag aactgctcga
    10981 ctttgcccag cagtatgcct gccagaaagg gttaaccgcg accttacgag gataacaacc
    11041 atgaccacac tgaaactgga cacgctcagc gaccgcatta aagcgcacaa aaatgcgctg
    11101 gtgcatattg tgaaaccgcc agtctgtacc gagcgcgcgc agcactatac cgagatgtat
    11161 caacaacatc tcgataagcc gatcccggta cgtcgcgcgc tggcactggc gcatcacctg
    11221 gcgaatcgca ccatctggat caaacacgat gagttgatca ttggcaacca ggcaagcgaa
    11281 gttcgcgccg cgccgatctt cccggaatat actgtctcgt ggatcgaaaa agagattgat
    11341 gatctggcag atcgtcccgg tgctggcttt gcggtgagcg aagagaacaa acgcgttctg
    11401 catgaagtgt gcccgtggtg gcgcggtcag accgtacagg atcgctgcta cggcatgttt
    11461 accgatgagc aaaaaggtct gctggcgacc ggaatcatta aagcggaagg caatatgacc
    11521 tccggcgatg cgcacctggc ggtgaatttc ccgctgctgc tggaaaaagg tcttgatggt
    11581 ctgcgcgaga aagtagcgga acgtcgctcg cgcatcaatc tgacggtact ggaagatttg
    11641 cacggcgaac agttcctgaa agcgattgat atcgtgctgg tggcagtcag cgaacacatt
    11701 gaacgttttg cagccctggc gcgtgaaatg gccgcgaccg aaacccgcga aagccgtcgc
    11761 gatgaactgc tggcgatagc agaaaactgc gatcttatcg cccaccagcc gccgcagact
    11821 ttctggcagg cgctgcaact gtgttacttc atccaattga tattgcagat cgaatctaac
    11881 ggtcactcgg tatcgtttgg tcgtatggac cagtatctct acccgtacta tcgccgcgac
    11941 gttgaactca accagacact ggatcgcgaa cacgccatcg aattgctgca tagctgctgg
    12001 ctgaaactgc tggaagtgaa caagatccgc tccggctcac actcaaaagc ctctgcggga
    12061 agtcctctgt atcagaacgt caccattggc ggacaaactc tggttgatgg tcaaccaatg
    12121 gacgcggtga atccactctc ttacgctatc ctcgaatcct gcggtcgcct gcgttccact
    12181 cagcctaacc tcagcgtgcg ttaccatgca ggaatgagta atgatttcct cgacgcctgc
    12241 gtacaggtga tccgttgcgg cttcgggatg ccggcgttca acaacgacga aatcgtgatc
    12301 ccggaattta ttaaactcgg tattgaaccg caggacgctt acgactacgc agcgattggt
    12361 tgtatcgaaa ccgccgtcgg tggcaaatgg ggctatcgct gtaccggcat gagctttatc
    12421 aacttcgccc gcgtgatgct ggcggcgctg gaaggcgggc gtgatgccac cagcggcaaa
    12481 gtgttcctgc cacaagaaaa agcgttgtcg gcaggcaact ttaacaattt cgatgaagta
    12541 atggacgcgt gggatacgca aatccgttat tacacgcgta aatcaatcga aatcgaatat
    12601 gtcgtcgaca ccatgctgga agagaacgtg cacgatattc tctgctcggc gctggtggat
    12661 gactgtattg agcgagcgaa aagtatcaag caaggcggcg cgaaatatga ctgggtttct
    12721 ggcctgcagg ttggcattgc caacctcggc aacagcctgg cggcagtgaa gaaactggta
    12781 tttgaacagg gcgcgattgg tcagcaacag ctggctgccg cactggcgga tgacttcgat
    12841 ggcctgactc atgagcaact gcgtcagcgg ctgattaacg gtgcgccgaa gtacggcaac
    12901 gacgatgata ccgtcgatac gctgctggct cgcgcttatc agacctatat cgacgaactg
    12961 aaacagtacc ataacccgcg ctacggtcgt ggtccggttg gcggcaacta ttacgcgggt
    13021 acctcgtcta tctccgctaa cgtaccgttt ggcgcgcaaa ctatggcaac accggacggg
    13081 cgtaaagccc acaccccgct ggcagaaggc gcaagcccgg cctccggtac tgaccatctt
    13141 ggccctactg cggtcattgg ctcagtgggt aaactgccta cggcagcgat tctcggcggc
    13201 gtgttgctca accagaaact gaatccggca acgctggaga acgaatctga caagcagaaa
    13261 ctgatgatcc tgctgcgtac cttctttgaa gtgcataaag gctggcatat tcagtacaac
    13321 atcgtttccc gcgaaacgct gctggaggcg aaaaaacatc cggatcagta ccgcgatctg
    13381 gtagtgcgtg tcgcgggcta ttccgccttc ttcaccgcgc tctctccaga cgcacaggac
    13441 gatatcatcg cccgtactga acatatgctg taatctcctc ggcccggcgg cgtcgtcgcc
    13501 gggctaaatc attcacatca tcaatttcat ccttactttc attcgaaata taatttgtgc
    13561 tctgcgtcac attgttatta cactgttacg gtttcatttt cgagccagga gcacacctat
    13621 gagcgtaaaa gttatcgtca cagacatgga cggtactttt cttaacgacg ccaaaacgta
    13681 caaccaacca cgttttatgg cgcaatatca ggaactgaaa aagcgcggca ttaagttcgt
    13741 tgtcgccagc ggtaatcagt attaccagct tatttcgttc tttcctgagc taaaggatga
    13801 gatctctttt gtcgcggaaa acggcgcact ggtttacgaa catggcaagc aactgttcca
    13861 cggcgaactg acccgacatg aatcgcggat tgttattggc gagttgctaa aagataagca
    13921 actcaatttt gtcgcctgcg gtctgcaaag tgcatatgtc agcgaaaatg cccccgaagc
    13981 atttgtcgca ctgatggcaa aacactacca tcgcctgaaa gctgtaaaag attatcagga
    14041 gattgacgac gtactgttca agttttcgct caacctgcca gatgaacaaa tcccgttagt
    14101 gatcgacaaa ctgcacatag cgctcaatgg cattatgaaa cccgtcacca gtggttttgg
    14161 ctttatcgac ctgattattc ccggtctaca taaagcaaac ggtatttcgc ggttactgaa
    14221 acgctgggat ctgtcaccgc aaaatgtggt agcgattggc gacagcggta acgatgcgga
    14281 gatgctgaaa atggcgcgtt attcctttgc gatgggcaat gctgcggaaa acattaaaca
    14341 aatcgcccgt tacgctaccg atgataataa tcatgaaggc gcgctgaatg tgattcaggc
    14401 ggtgctggat aacacatccc cttttaacag ctgacctctc taccggagcc gtgcgctccg
    14461 gttttccctt ctttttacat cctgtacatc acatcaaatc attaaacttg cattaactta
    14521 tttataactt atattgtcgc atgcaaatca gaatggtttc gaatgaaagt taatgagagg
    14581 tggttatggc ttctactttt accagcgaca cattgcctgc cgatcacaaa gcagctatcc
    14641 gtcagatgaa gcacgcgctg cgggcgcagc ttggcgacgt ccagcagatc tttaatcagc
    14701 taagcgatga cattgccacg cgagtggctg aaatcaacgc actcaaagca cagggcgatg
    14761 ccgtctggcc ggtgctgtct tatgccgata tcaaagcagg tcatgttact gcagagcagc
    14821 gcgaacagat taaacgtcgc ggttgtgcgg tgataaaagg ccatttcccc cgcgaacaag
    14881 cgctaggctg ggatcagtcg atgctggact atctggaccg caaccgcttt gacgaggtct
    14941 acaaaggccc cggcgataat ttcttcggga cgctcagcgc ttcacgtccc gagatttacc
    15001 ccatctactg gtcgcaggcg caaatgcagg cccgccagag tgaagaaatg gcgaatgcgc
    15061 agtcgtttct caatcgtctg tggacatttg aaagtgatgg aaagcaatgg tttaacccgg
    15121 atgtgagcgt catctaccct gaccgtatcc gccgccgtcc gcccggaacg acctccaaag
    15181 gtcttggagc gcataccgac tccggggcgc tggaacgctg gctgcttcca gcgtatcagc
    15241 gcgttttcgc caacgtcttt aatggcaatc tggcgcaata tgatccctgg catgcggcac
    15301 atcgtacgga agttgaagag tacacggtgg acaacaccac caaatgttcc gtgtttcgga
    15361 cattccaagg ctggacagcg ctctctaata tgctgcctgg tcaggggctg ctgcacgttg
    15421 tgcccattcc tgaagccatg gcgtacgtac tgttacgtcc gctgcttgat gatgtgccgg
    15481 aggatgaact gtgcggcgta gcgcccggaa gagtgttgcc ggtatcagag caatggcatc
    15541 cactgttaat tgaggcgtta accagcattc caaaactcga ggccggagac tccgtctggt
    15601 ggcactgcga cgtcatccat tccgttgccc ccgttgaaaa tcaacagggt tggggcaacg
    15661 tgatgtacat tcctgcggca ccaatgtgcg agaaaaatct tgcctacgcg cacaaggtga
    15721 aggccgcact ggaaaaaggc gcatcgccgg gcgacttccc gcgcgaggac tatgaaacaa
    15781 actgggaagg acgctttacg cttgccgacc tcaacattca cggtaagcga gcgttgggaa
    15841 tcgacagctg agtgtcgtac agcccttccc gttccacggc ggtgttccct tttcctgggc
    15901 gaacccgccg cactgactcc cgcaccgcca atcgtccgtg cagcagcagc gagcccaccg
    15961 gagcatccgg gcgaatcagc cgctgttgca acatttgcac cgcttcattg cctagctcat
    16021 cgcgtggcac atgcacagcc gtcaggggaa catcctcgat tgccgcaagg ttaaagccgt
    16081 cgatactcat caccgagaca tcctgtggta cgcgcagacc acgacgatgt agcgcattta
    16141 ccgttcccgc cgccataaaa tccccgccga ccagaaacgc cgtcggcaac gtcttgccat
    16201 tggcggcatc cagccacgct ccgacctttt cttccgcttc tttagcgcta aaactgctga
    16261 aattcagcaa atgacggtca tggtgaaagg ccagattatg ctgacgccat gattctttaa
    16321 tccctgcaaa ccgctgctcc atagtgtagc ggcgcaggca catcacattc atcacctccc
    16381 ggtgacccat ttcaaacaga tagcgggcgg cgaacgcgcc aattaaccga tgatccggcg
    16441 caatcgccgg gaggcgcatt cgttcatcac ggcagttgat cagcacacaa ggcttagcga
    16501 gatcggcggc cagatcatgg atgtgcggat cgtcaatccc cagcaggatc gctgcctgcg
    16561 tatctgcttc attcatccgc gagagaaaga gcgcgggatc gctgtcaaac tcctccagcg
    16621 cgcagtaacg cagccgcact tcatgcggcg ataacgcttt gctgatgctc tggatcacac
    16681 ggtaataaaa gatatcagat cgttggtcaa aagcgcgttg cggcgcgaaa ataatcaggt
    16741 tgttcagcaa catacgacct gctgccatgc catccatcac gcccaactca cgcgcgcagg
    16801 ccagcacctg ttcgcgagcg cgtgcactgg tattcgcttt gccagccagc acccgcgaaa
    16861 ccgtactcgg cgatagcccg gtacgtgcgg cgatatcggc gattttcagc ttcttgtcca
    16921 ttttgtgatc tgcctctaat tcggaaatga cagaattttc atgagcaaaa aagcccattc
    16981 tgtctggcat taaatccatt tcagtaatgt atatcagtca ctcatggaaa aacgtgcaca
    17041 aaacccgctt tctttcgctt ttagggtctc ttactgtgaa ttggtagaac aacttccata
    17101 caaaaataat ggaaaaacga acaataaaga ctttatttat caaaaagtta aaagtggtgt
    17161 aacaactcca ttctgacgtt gccgcacaaa ccctatacca ttctgtggag tgcatatgag
    17221 tcagggtatc aataacgata tgactgccag taaaccgcgc catatcgtca aaaatctgcg
    17281 ttggtggata ctggtccttt ttttgctcgg tgttactgtc aactacatca cgcgtaactc
    17341 gctgggtatt ctcgcccccg aactgaaaag cagcctaggg attaccaccg aacaatattc
    17401 ctggattgta ggtgctttcc agctcgccta taccctattc caaccgttgt gcggatggct
    17461 gattgacgtc attggcctca aactgggctt tatgatctgc gcgggactgt gggcactggc
    17521 ctgtctgatg catgcggggg cgggaaactg ggtacatctg gcgctgttgc gcttctttat
    17581 gggcggtgcg gaagccgcgg caaccccagc aaacgccaaa accatcggtg aatggtttcc
    17641 aaaatcagaa cgcccgatcg ccgcaggctg ggcaggcgtc ggtttttcca tcggtgccat
    17701 gctggcccca ccgattatct attttgccca cgcttcattc ggctggcagg gtgcatttat
    17761 gttcactggc atcctggcga tgctgtgggt ggtgctgtgg tgggcgttct atcacagccc
    17821 ggataagcat cctaacctca gcaaagccga gctggacttt attcgccagg ataacgaagc
    17881 gcccccggtc aagttaccct ttctgaccgc actgaaaacc gtgggcaaaa ataaacgctt
    17941 ttacggtatc gctatcccgg cctttatggc agaaccggcg tgggcggtga tgagtttctg
    18001 ggtgcctcta tacctgtcca aaacctatgg catggacctg aagcagatcg cattattcgc
    18061 ctggttgccg ttcctcgccg ctgatcttgg cagtattgcc agcggctatc tgaccaaact
    18121 ttacacccgc ctgtttgggc tgagtcgtgt gaactccgtt gtcgcatcat cggtcagtgg
    18181 cgcattcttg atggtttcgc tggcggtgat ggcctttacc aataacccgt atatcgccat
    18241 cgtccttatc tccatcggcg gcttcggtca ccagatcatc tcctgcatgc tgagcgcact
    18301 ggtggtggaa tccttcgaca aaggtcagat ggccactgtt aacggcatgc gcggttctgc
    18361 cgcgtggatc gcaagtttcc tgttctccct gattatcggg gttaccgctg acaaaatcgg
    18421 ctttaacccc ctgtttatcg ccatgggctt ctttgacctg attggcgctt tcttcctggt
    18481 cacctttatt gctgaacgtc gcgcaaaacg cgcctgagag tggatgtaaa catgaaaacg
    18541 ctgaaaaact ggaccctgca caaacagtct gcccaccacg ttgaactgct ggtcgatggc
    18601 cagcatatcc tgtgcctgta cgtgctggaa gaaaatctgt tccgcgtgct gttaaaacgc
    18661 aaaggcgagc tggcgctaga tcgcacctgg agcattgctc cggcgcagga tgtgccatgg
    18721 gaagggcgca gccgcgaaga tctcagcggc tttagcctgc ccgcgtggac agtgatccag
    18781 cacgacgaca ccgtggttat cgccaccgag tcactgcgtg caaccgttca ccagccgctg
    18841 tggctggagt ggcactatcg taacaacgaa ggcgaatggc tgccgttggt gaatgaccgc
    18901 ccgaccagcg cttatctgct gaatgcccac ggcgatggcg tggcacacta tcagagccgt
    18961 cgtaaagatg aacgctttta tggcctcgga gataaagccg gcgatctgca acgcaacggt
    19021 aaacgttacg agatgcgtaa cctcgacgcg atgggctaca acgccgttag tactgacccg
    19081 ctgtacaaac acattccatt caccatcacc cgccgcgatg acattagctt cggcctgttt
    19141 tatgacaacc tcagcagctg ctggctggat ctgggcaacg agatcgacaa ctaccacacc
    19201 gcctatcgcc gctggcaggc ggaagcgggt gatatcgact actacctgtt taccggcgaa
    19261 cgcgtacttg atatcaccaa agccttcgta cgcctgaccg gaaaaaccct gttcggaccg
    19321 aaatggagcc tgggctacag cggttcaact atgcactaca ccgatgcgcc ggacgcacaa
    19381 aatcagttga tgaactttat ccgcctgtgt gaagaacatg caattccctg cgattcgttc
    19441 cagctctctt ccggttatac ctcaattaac ggcaaacgct atgtgttcaa ctggaactac
    19501 gacaaagtgc cgcagccgaa agtgatgagc caggcatttc acaatgctgg tttgcacctg
    19561 gccgccaata tcaagccgtg cctgttgcag gatcacccac gctataacga agtggcagaa
    19621 agaggactgt tcatccgtga ttcagaaaca gatgaaccag aacgttccag cttctgggat
    19681 gacgaaggtt ctaacctcga ctttactaat ccgcagacca tccagtggtg gcaggaaggc
    19741 gtgaccacac agcttctgga gatgggtatc gactccacct ggaacgacaa caacgaattc
    19801 gaagtatggg acggtgaagc acgctgtcac ggctttggtc agacgatcgc gatcaaacat
    19861 attcgcccag tgatgccact gctgatgatg cgagcctcga tggaagccca gcagcgtttc
    19921 gctccggaaa aacgtccgta cttgatctcc cgctccggct gcgcgggaat gcagcgctac
    19981 gtgcagacct ggagtggcga taaccgcact agctgggaca ctctgcgcta caacacccgc
    20041 atgggtctgg ggatgagcct ctccgggctg tataacctcg gtcacgacgt cggcggtttc
    20101 tccggtgata agccggaccc ggaactgttt gtacgctggg tacaaaacgg cgtgatgcat
    20161 ccgcgcttta cgattcactc atggaacgat gaccataccg taaacgaacc gtggatgtat
    20221 ccgggcgtca ctccggcgat ccgcagcgct attgagttgc gctatcgcct gctgccctac
    20281 ttttataccc tgatgtggca ggcatacgcc gatgatgaac caatgctacg tccgactttc
    20341 cttgaccacg agcacgacgt tcagactttt gaagagtgtg atgacttcct gcttggtcgc
    20401 gatattttgg ttgccagcgt cgtagagcaa ggcgagcgcc agcgccgtgt atggctgcca
    20461 gataacgaaa ctggctggta cgatttctac aacggcgaat ggttcagtgg cggtcagtgg
    20521 atcaccctcg acgcaccgct ggagaaactg ccgctgatgg ttcgtgccgg ggctggtcta
    20581 ccgctcagca aacgcatcac ctatgtgagc gcagaacaag acgatacccg cgaactgaaa
    20641 ctgttcccgc tgaaaggtgt gggcacaacg tccggcctgc tgtttgaaga cgatggcgaa
    20701 agctggggct accaaactgg taatgcgctt tgggtggaat gggaaatggt ttgcgatggg
    20761 gcgaccatca acctgaaagt caacgcgcgc ggggattatc gtcctgcatg gagtgcactg
    20821 aaagtgtcat taccattgga agaaaaacgc acgctgctgg tcaatggtgt tgaagggagt
    20881 gaatggatgc gttaaatcat taccggggcc gggaggcgac tgcaccttac ccggtccagc
    20941 catcgaatcc tttacctcag tatttcacgc tgcaggaagg cagcaaacct gtaaaccccg
    21001 ggagcttaaa taagtaagtg accggggtga acaggtgaag ccaatgcatc tgccgcatga
    21061 aagacggcgg ggaattaagc gtcgatccct ttactacgca ggtaatcttc gtaattaccg
    21121 ctaaagtcga tcgcgcgttc cggggtgatt tccagaatgc gggtcgccag ggagcttacg
    21181 aactcacggt cgtgtgaaac aaagatcagc gtgccctgat acagttccag cgccatgttc
    21241 agtgactcga tggattccat atccaggtgg ttggtcggtt cgtccatgat cagaatgttc
    21301 ggcttctgca tcattaactt accaaacagc atccgccctt tttccccacc ggaaagcact
    21361 ttagctggct ttttgatgtc gtcctggctg aacagcaaac gaccgagaat gctgcgtacc
    21421 gcctgctcgt catcgccttc ctgcttccac tgactcatcc attcgaacac ggtcagatca
    21481 ttttcaaact catattcgtg gtcctgggcg tagtaaccaa tgcgcgcgtt ttcagaccat
    21541 tttacggtgc cgctgtccgg ttgcagatcg cccaccagcg ttttcagcag cgttgattta
    21601 ccgacgccgt tggtacccag taccgcaagt ttttcaccga cctccagcag caggttgaga
    21661 tttttaaaca gtggaccgtt atcaaaccct ttggtcagac cttccacttc cagcgcgtta
    21721 cggaacagtt tcttatcctg ttcaaaacgg atgaacgggt tctgacggct ggaggctttc
    21781 acctcttcca gtttgatttt atcaatctgg cgcgcgcgcg aagttgcctg gcgagatttc
    21841 gaggcgttgg cgctaaagcg gctaacgaaa gattgcaact cagcaatctg cgctttcttc
    21901 ttggcgttat cggccagcag acgttcacgc gcctgggtcg ccgccgtcat gtactcatcg
    21961 tagttacccg gataaacgcg cagctcgccg taatccagat ccgccatgtg ggtacagacc
    22021 atgttaagga agtgacggtc gtgcgagatg atgatcatgg tgctgtcacg ctcgttcagc
    22081 acctgttcca gccagcgaat ggtgtcgatg tcgaggttgt tggtcggttc gtcgagcagg
    22141 agaatatccg gatcagcaaa cagcgcctgc gccagaagca cacgcagttt ccagccagga
    22201 gcaacttcac tcatcgggcc gtagtgctgt tccactggaa ttcccacgcc aagcaacagt
    22261 tcaccggcgc gagcttccgc agagtaaccg tccatttcgc cgtatttaac ttccagatcg
    22321 gccactttat agccgtcttc ctcactcatt tccggcaaag catagatgcg gtcacgctcc
    22381 tgcttcactt cccacaactc tttatgcccc atgatcaccg tatccagcac agtgaactct
    22441 tcaaaggcaa actgatcctg acgcagctta ccaatgcgct cgttgggatc gagggaaacg
    22501 ttacccaaag tcggctctaa gtcgccgccg aggatcttca taaaggtgga tttaccacta
    22561 ccgttcgcgc caatcaggcc gtaacggttg ccgccgccga atttgacgga aatgttttca
    22621 aacaacggct tactgccgaa ctgcatggtg acgttactgg aaactaacac aggcgtatcc
    22681 tgaaaagaga tatgacaaac cgcgcattat gccataagtc tgaaagaaga tcctgctcct
    22741 cgcttaaggt ctacactcaa gccaaaggca aaaaatgtga tttcgtacac atctgatttc
    22801 actgtgagct ggaatgaact tataatgcgc ttccaatact ctaatattct caacccaatg
    22861 gcctgccagg cacaaaatct cgcttaacat gaatatgaaa ttgaaaacat tattcgcagc
    22921 ggccttcgct gttgtcggct tttgcagtac cgcctctgcg gtaacttatc ctctgccaac
    22981 cgacgggagt cgcctggttg gtcagaatca ggtgatcacc attcctgaag gtaacactca
    23041 gccgctggag tattttgccg cggagtacca gatggggctt tccaatatga tggaagcgaa
    23101 cccgggtgtg gataccttcc tgccgaaagg cggtactgta ctgaacattc cgcagcagct
    23161 gatcctgccg gataccgttc atgaaggcat cgtcattaac agtgcagaga tgcgtctgta
    23221 ttactatccg aaagggacca acaccgttat cgtgctgccg atcggcattg gtcagttagg
    23281 caaagatacg cctatcaact ggaccaccaa agttgagcgt aagaaagcag gcccgacctg
    23341 gacgccgacc gccaaaatgc acgcagagta ccgcgctgcg ggcgaaccgc ttccggctgt
    23401 cgttccggca ggtccggata acccgatggg gctgtatgca ctctacatcg gtcgcctgta
    23461 cgctatccat ggcaccaacg ccaacttcgg tatcggcctg cgtgtaagtc atggttgtgt
    23521 gcgtctgcgt aacgaagaca tcaaattcct gttcgagaaa gtaccggtcg gtacccgcgt
    23581 acagtttatt gatgagccgg taaaagcgac caccgagcca gacggcagcc gttatattga
    23641 agtccataac ccgctgtcta ccaccgaagc ccagtttgaa ggtcaggaaa ttgtgccaat
    23701 taccctgacg aagagcgtgc aaacagtgac cggtcagcca gatgttgacc aggttgttct
    23761 tgatgaagcg attaaaaacc gctccgggat gccggttcgt ctgaattaat cttcaaaact
    23821 taaagcaaaa ggcggactca taatccgcct tttttatttg ccagacccta gttggccggg
    23881 agtataacta acaaaacata tcccaccaac gccgcccaca acagcatcgg tattgaatag
    23941 agatggaaac gccaccagat gcggcgatcg ttcgccatac gtagcgcaat caaatttgcc
    24001 agcgatccgg gtaataaccc aaagccacct acgtttaccg cccataccag taataaagac
    24061 ggcggcacat agttcagcaa caatatggta ctcggcacgt tactgatcac ctgcgataaa
    24121 ccgattgccg ttaaccataa cccgggttca gatagatgac tcacgttacc caacacgcct
    24181 tgcaacgctg gaagctgggt cagtaaatgg acgtcgataa acatcgccat aaacaccagc
    24241 agcagcgtcc agtccacact gagcaccacg cgacgtgcca gcagcgcaaa gcctgccgcc
    24301 acaatcacca gtccccacag ctcttgtttg aactccagcg ccgtcagaaa gacgatatac
    24361 agccccagac aactccacac cagccgcggt ttccactccg gtgtttgcac ccccgtatgg
    24421 tattgcagcg cctttccagg gaaacaacac cagcacagga gcaggagcgt cagcatcatt
    24481 gcgccagcca gcggtgccat ttgggcaata aatccggcaa acgaaagacc agaacgtccc
    24541 cagataagaa tattttgcgg gttgccaatt ggcgtcagta gcgaaccagc gttgactgcc
    24601 agcgcctcaa aaataatcag ccgattaacc gggatctcac acagtctttt tagcgtgata
    24661 gtcagcggaa caacaataaa cagcgcgaca tcgttggtca gaaaggtaga aagcagcgcc
    24721 gccgccagca ccataaacat cgccagccga cgctccgtag caaagcggcg caccattttg
    24781 cgccccagca catcaaaata accgcttaac tccacacctt tggtcagcag catcaggccg
    24841 cttaaggtga tgatggtgtg ccagtcgata gcagcaggcc aggatttcgg tgcaaagggc
    24901 acgaaaaagc ttaatccgat accaacaaga attaataact gaaaaaaacg atcgccttgc
    24961 agcgtgcgta aaaaaggcag gctcatttgg caccgtgttt ctgggtaaac aaacgaaaag
    25021 cgtcgagcgt ctcttcacta acatggtgct ccatgccttc cgcgtcgcga cgggcgattt
    25081 ccggactgac gcccaacacc aacaagaaat tttcgactat ctgatgtcgc tcgcggcttt
    25141 cctgcgccag cttctctcct tctgccgtta aaaacacgcc acgccagggg atcatttcaa
    25201 tcagccccat ggttgccagc cgcttaagca ttttagccac cgtcggttgc gaaactccca
    25261 gacgagcagc catgtccacc tgacgagctt cccccacttc cctgatcaag tcagaaatca
    25321 gctcaacgta atcatcaata agctcgcgcc gatgcgcctc tctgacctgg cggaaccctt
    25381 caacgtgctc ttccacgttc actaactgcg tcactttttt tgctgttggc gtacctgcgc
    25441 gacgactcat tgtgcttcct cattatggtg acgcatacat agcgttctca tttcgtgcgc
    25501 acattgtaaa ccagagttgc gaaggtacaa aaaattaacg ttttagcaat agctacataa
    25561 tatagcctgt gctatatctg tatgtaatgc aatcatccct caaggatcga cgggattagc
    25621 aagtcaggag gtcttatgaa tgagttcaag aagtgtatgc gcgtgtttag tcattctccc
    25681 tttaaagtac ggttaatgct gctctctatg ttgtgcgata tggtcaacaa caaaccgcag
    25741 caagataaac cttccgataa atagcggcgt cgcggtacgc cgcttcactc ctgcttttat
    25801 gcaggcataa cgcgttttgg tctgaaaaac cccacttttt gtcggatttg caatcccctt
    25861 cgcaaaagat ttgttcgtca gtagttgacc tgaacggcgg ctcgctctat cttcttgcag
    25921 ccctgcgtat attgcggctc gcggatgcgg acccctttcc cctcttcacg cactcttgca
    25981 ggtattgacc cttgacgcca gggtaagcac atggcgtttg ttacgatagt ggcatatgaa
    26041 tttaaccctc aaagaatcgc ttgttacccg tagccgggta tttagcccgt ggactgcgtt
    26101 ctacttttta cagtcgctat taattaacct cggcttaggt taccccttca gtttgctcta
    26161 caccgctgcg tttacggcta ttttgctttt gctatggcga acattgcctc gcgtacaaaa
    26221 agttctggtc ggtgtcagtt cgctggtggc ggcttgttat ttcccttttg ctcaggccta
    26281 cggcgcgcct aatttcaata cattgctggc attgcactcc accaatatgg aagagtcgac
    26341 cgaaatcctg acgatttttc cgtggtacag ctacctggtc ggcttattta tttttgcgct
    26401 cggcgtaata gcaatcaggc gaaaaaaaga gaatgaaaaa gcgcgctgga ataccttcga
    26461 cagcctgtgt ctggtattca gtgtggcgac attttttgtt gctcccgtgc aaaacctggc
    26521 ctggggtggc gtatttaaac tgaaagatac tggctatccg gtatttcgtt ttgctaagga
    26581 tgtcatcgtc aataataacg aggtgattga agagcaagaa cggatggcaa aactttccgg
    26641 aatgaaagat acctggacgg tcactgccgt taagccgaag tatcagacct atgtggtggt
    26701 gatcggcgaa agcgcgcgtc gcgatgccct cggtgccttt ggcggtcact gggacaatac
    26761 cccgtttgcc agcagcgtta acggtttgat atttgctgac tacattgccg ccagtggctc
    26821 cacgcagaaa tcacttggct taacgctcaa tcgcgtagtc gatggcaaac cacagtttca
    26881 ggataacttt gtcaccctgg caaatcgcgc gggcttccag acctggtggt tttccaacca
    26941 gggccaaatc ggcgaatacg atactgctat cgccagcatc gccaaacgtg cagatgaagt
    27001 gtacttcctg aaagaaggta attttgaagc agataaaaac acgaaagacg aagcgttact
    27061 ggatatgacc gctcaagtgc tggcgcaaga gcactcgcaa ccgcagctga ttgttctgca
    27121 tctgatgggc tcgcatccgc aggcctgcga caggacacaa ggcaaatacg aaacctttgt
    27181 gcaatcgaaa gaaacgtcgt gctatctcta taccatgacg caaacggacg atttactgcg
    27241 caagctgtac gatcagttac gcaacagcgg cagcagcttc tctctggttt acttttctga
    27301 ccacggtctg gcctttaaag agcgcggcaa agacgtgcaa taccttgccc atgatgataa
    27361 atatcagcaa aatttccagg tgccttttat ggtcatttcc agcgacgata aagcgcatcg
    27421 ggtgattaaa gcccgccgct cagccaatga cttcttaggc tttttctcgc agtggacggg
    27481 aattaaagcg aaggaaataa atatcaaata cccgtttata tctgagaaga aagccgggcc
    27541 gatatacatc accaacttcc agttacagaa ggtggattac aaccatctcg gaaccgatat
    27601 tttcgacccg aaaccttaaa acaaaaaatc cgccccgaga g
//
LOCUS       DAZPLI010000043        27468 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZPLI010000043 DAZPLI010000000
VERSION     DAZPLI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27468
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.043"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..81)
                     /locus_tag="ACHUU3_001750"
     CDS             complement(<1..81)
                     /locus_tag="ACHUU3_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR2081838.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTL"
     gene            complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="ACHUU3_001751"
     CDS             complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="ACHUU3_001751"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFR2081839.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="ACHUU3_001752"
     CDS             complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="ACHUU3_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFR2081840.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="ACHUU3_001753"
     CDS             complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="ACHUU3_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR2081841.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCIGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5734..6231
                     /gene="msrC"
                     /locus_tag="ACHUU3_001754"
     CDS             5734..6231
                     /gene="msrC"
                     /locus_tag="ACHUU3_001754"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR2081842.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6328..7026
                     /gene="proQ"
                     /locus_tag="ACHUU3_001755"
     CDS             6328..7026
                     /gene="proQ"
                     /locus_tag="ACHUU3_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR2081843.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7046..9094
                     /gene="prc"
                     /locus_tag="ACHUU3_001756"
     CDS             7046..9094
                     /gene="prc"
                     /locus_tag="ACHUU3_001756"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR2081844.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9286..10167
                     /gene="htpX"
                     /locus_tag="ACHUU3_001757"
     CDS             9286..10167
                     /gene="htpX"
                     /locus_tag="ACHUU3_001757"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR2081845.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10213..11276)
                     /locus_tag="ACHUU3_001758"
                     /pseudo
     CDS             complement(10213..11276)
                     /locus_tag="ACHUU3_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11453..12244
                     /gene="kdgR"
                     /locus_tag="ACHUU3_001759"
     CDS             11453..12244
                     /gene="kdgR"
                     /locus_tag="ACHUU3_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR2081846.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12387..12626)
                     /locus_tag="ACHUU3_001760"
     CDS             complement(12387..12626)
                     /locus_tag="ACHUU3_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR2081847.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12785..12928
                     /gene="mgrB"
                     /locus_tag="ACHUU3_001761"
     CDS             12785..12928
                     /gene="mgrB"
                     /locus_tag="ACHUU3_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR2081848.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13003..13290
                     /locus_tag="ACHUU3_001762"
     CDS             13003..13290
                     /locus_tag="ACHUU3_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR2081849.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            13960..14103
                     /locus_tag="ACHUU3_001763"
     CDS             13960..14103
                     /locus_tag="ACHUU3_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR2081850.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14116..14325
                     /gene="cspE"
                     /locus_tag="ACHUU3_001764"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14116..14325
                     /gene="cspE"
                     /locus_tag="ACHUU3_001764"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR2081851.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14491..15300
                     /gene="rlmA"
                     /locus_tag="ACHUU3_001765"
     CDS             14491..15300
                     /gene="rlmA"
                     /locus_tag="ACHUU3_001765"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR2081852.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15297..15863)
                     /gene="mntP"
                     /locus_tag="ACHUU3_001766"
     CDS             complement(15297..15863)
                     /gene="mntP"
                     /locus_tag="ACHUU3_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR2081853.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(16292..16750)
                     /locus_tag="ACHUU3_001767"
     CDS             complement(16292..16750)
                     /locus_tag="ACHUU3_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR2081854.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(16805..17656)
                     /gene="manZ"
                     /locus_tag="ACHUU3_001768"
     CDS             complement(16805..17656)
                     /gene="manZ"
                     /locus_tag="ACHUU3_001768"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR2081855.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(17669..18469)
                     /gene="manY"
                     /locus_tag="ACHUU3_001769"
     CDS             complement(17669..18469)
                     /gene="manY"
                     /locus_tag="ACHUU3_001769"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR2081856.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(18532..19503)
                     /gene="manX"
                     /locus_tag="ACHUU3_001770"
     CDS             complement(18532..19503)
                     /gene="manX"
                     /locus_tag="ACHUU3_001770"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR2081857.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            19820..19978
                     /gene="yoaL"
                     /locus_tag="ACHUU3_001771"
     CDS             19820..19978
                     /gene="yoaL"
                     /locus_tag="ACHUU3_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR2081858.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            19966..21522
                     /gene="yoaE"
                     /locus_tag="ACHUU3_001772"
     CDS             19966..21522
                     /gene="yoaE"
                     /locus_tag="ACHUU3_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR2081859.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(21526..23124)
                     /locus_tag="ACHUU3_001773"
     CDS             complement(21526..23124)
                     /locus_tag="ACHUU3_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR2081860.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(23255..24619)
                     /gene="sdaA"
                     /locus_tag="ACHUU3_001774"
     CDS             complement(23255..24619)
                     /gene="sdaA"
                     /locus_tag="ACHUU3_001774"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR2081861.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(24803..25381)
                     /locus_tag="ACHUU3_001775"
     CDS             complement(24803..25381)
                     /locus_tag="ACHUU3_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR2081862.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(25385..26746)
                     /gene="pabB"
                     /locus_tag="ACHUU3_001776"
     CDS             complement(25385..26746)
                     /gene="pabB"
                     /locus_tag="ACHUU3_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR2081863.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            26820..26999
                     /locus_tag="ACHUU3_001777"
     CDS             26820..26999
                     /locus_tag="ACHUU3_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR2081864.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(27119..27418)
                     /locus_tag="ACHUU3_001778"
     CDS             complement(27119..27418)
                     /locus_tag="ACHUU3_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR2081865.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
BASE COUNT         7066 a         6773 c         6940 g         6689 t
ORIGIN      
        1 taacgttcga tcacccggcg attcaccgtg taattcgccg tcgtcgattt caaaagcgcc
       61 tatgcgcaca ctggttttca tcttcgtgct ctccatttag ctgccggttg tggtacgacc
      121 agtccgatcg caccattttt cagcctagtt gaggcagccg aatgtgccag tcaaaaagtg
      181 cgcttttttt gtgcgctgtc aggcgttacc ggtaaaaagt ttcccatcgc gcaccagttc
      241 acgcggatag ctgtttttca atcgcgaacc aatccgtttg gctaaaccaa tcggttgatg
      301 ctggaaagta accaacacgt catccgccac tggcgcggct tgcgggtaaa catcgcgccc
      361 gcgataccac tcctccgctt cctgcggtgt cagttcaaaa gcgttcacat tgtcgggggt
      421 ggcaagggca ataactgctt catgctgcca gcgataacct ttgttgtgcg tttcggcaag
      481 tttaatcccc aagcgagaaa atcggacttt accgatcagg gcttcaatgc ccaccgggaa
      541 caaccacagt tctttgtcac gctgccagag gcgcaggttt tcatcccagt ttaagccaac
      601 acctgcagcc gcctgacgaa tttgtccagc ttcgcgatct ttcactgggc tgaacggaaa
      661 attaccgact ttgtatttgg gggcgggtaa ggcgggaatc gcctgagttt tacgcagacg
      721 agcaacgaag aagccttcgc agtcgtaaat ttgtgggaaa acatgcaaaa agccttcctc
      781 ggtcagcgcc ttgtttgcac cagggaagag atcaccaagc ggtaaaaact ctactgcgtc
      841 ggggtaagtc tctttcagcc acaggcaaac ggcttcgttt tcttcctggt ttaaggtaca
      901 ggtcgagtaa accagcgtac cgccagggcg taatgcatga aaggcgctgt cgatcagttc
      961 ccgttgggta gctgcgattt cctgattgct ttctggtgac cagtttttta gcgcatcggg
     1021 atctttacgc accacgcctt cgccggagca gggagcgtcc agcaaaatgg catcaaacat
     1081 ttctggcact gccgcaccaa acacgcggcc atcaaaatgt gtgagcgcaa cattactgat
     1141 accacagcgg ctgatattgg catgtaacac ttttacccga ctggcggaaa actcattggc
     1201 aagaattgcc ccttcgttat tcatccgcgc ggaaatttgc gtcgttttgg agccaggcgc
     1261 ggcagcgaca tccatcaccc gctgtggtgc attaccgtca gcaaacaagg cggcgacggg
     1321 caacattgaa ctggcttcct gaatataaaa caggccactt aaatgctcgg cggtactacc
     1381 caatggcaat gcatcttcat tgtcgcgttc aatccagaaa ccttcttcac accacggaat
     1441 tggcgtaagc gtccagccat aaggagcggt taattgcagg aaatcagcaa cggagatttt
     1501 cagcgtatta acgcgaatgc tgcggcgcaa cgggcgctga caggcggcaa gaaaatcatc
     1561 aaaagagagc gtcgaaggca tcgcttcgcg catttgcgtc agaaaggcgt ccgggaaata
     1621 aacggtgtgt tgggccacga gcatatacca ctggcaaaaa aaacaggcgc gcagtttagc
     1681 ataaacgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagtaccc
     1741 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgcttgcc ttcctgggct
     1801 ttcggtgcca gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaactgg
     1861 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     1921 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     1981 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2041 agaccacgga acagcacagg cgtaccaata cctaacgaac cggcttccgg tgcttcgaca
     2101 ataatgctta agccatccag gtaacgcgaa tcagtaatag tagcttcttg caactcaaag
     2161 tcacggcgag gattaccccg acctggttcg acattgatat acggctggag gatagtatct
     2221 agatgctcaa cgcccgcggc tgaaatttgc ggtgtgacca ccgagaagcg cgtaccgccg
     2281 cgggcgaagg tctggacata ctccggatag agcaccgcct ttgcctgtac ttcattgcgc
     2341 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     2401 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgccc accaaccgca
     2461 cgggccgctg tttcggaagc atacagaatt cgtttgtcgc ctttacgctg actggcgctg
     2521 gcaccgctga ggttatcgaa gctaatggct ccctttaatg ctctggagag cggggatgcc
     2581 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     2641 ctattgctgg tcagaaggtt gcgatactcc ggcttaatat gcagatcgat atcaaacgcg
     2701 ttagctcgcg gacgcacggt aatcacttca ccaacttcaa atttacggta cagcactacc
     2761 gagcctgcct gcacatccgg cagcgtctct gcactcaaac tcacggttgt ggtgggtaaa
     2821 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     2881 atctcacctt tatcgcctgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     2941 gcaccaagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3001 aatttgctat cgccttttac cagttcgcga tgctgaggct cgatggcgac ggtaaaggta
     3061 acgcctttgc tggtgagttt acgatcaata acctggccta cctgcacgcc gtgaagaatg
     3121 agcggctgac cagcatcaat accgtaactt tccggcgcgg tcagggtcag cgtcagaaca
     3181 tcaggttcat gcagcagtgc tttttcgcct ggcacaacaa cgaactcttt gcgtggctcg
     3241 ccatcgccgg gtaccaactc gaaggttttg ccagtcagca gggcgctgag attggcatcg
     3301 ctaagggata atttcggatt aagtaattcg atgcgggtat tttcacgaag cagggtaacg
     3361 acgctgggat taactgtcat ttccccagtg actttaccac caggatttaa atccagttta
     3421 gttagctgtc cgacttccag tccctgatac attaatggcg tcgagtcggc ggttaatccg
     3481 gccccactcg gcagttccag tttgattatt acgccacgct ggctatgggc gaggtcttca
     3541 tacagaccaa aggtatcttc cgcctcggca ggtttcgact cttcaggtga atcgaaggca
     3601 atcgcaccgt taaccagtgc cgccagactt tccagtttca ccttcgcgcc actgatactg
     3661 acgttggcat caacgccgga aacgttccag aaacggctac cttttttcac caggtcggta
     3721 aaacgccgct cgatcaggac atcaatcacc acgccttgct tgttgggatt gatggcatag
     3781 tcgtagactt ttcccacagg gatcttgcgg aaatagacca atgaaccgct gttcagcgaa
     3841 ccgagatcgg gggcttgcag gtggatcatc agatcgccat tgtccagtcg atatttcggt
     3901 tgggtatcga gtgcgacaaa gtgatcctgc tctttacctt tacctggcat catgccgata
     3961 tagttaccac cgacgagggc gtccagcccg gagacacctg ccaacgatgc ttttggcgtc
     4021 accagccaga actgagtctc ttcgcgcagc gcatctttca tatcggactt gatgctgacc
     4081 ttgacttcaa tcttacgaag atcgtcgctg aggctgatat cctgcactgt tccgacttca
     4141 acgccctgat aacgaacagg cgtacggccc gggacaatac catccgccga cataaagtcg
     4201 atggtgacgg tattaccccg gtcctgataa ctgtcccaaa tcagccaacc ggcaatcatt
     4261 agcgcgatga aaggcagcag ccagaaaggt gagatacggc gtttattttt aatctgcgct
     4321 tcagtcgtcg aagcgggcgt ttcctgactc atgtgcatcc caaagtaagc ggctgtccag
     4381 ccattccaca gcaagaatag tcaaaattac ggctgcgcca aaataaaacg cagccggtcc
     4441 catagtaaaa gcgaggatct gatcgcgatt aatcagcgac atggttaaag atatgacaaa
     4501 caggtcgagc atcgaccagc gtccaatcca ggtcaccatc cgcagtaaca gaatgcgtgt
     4561 gcgtaaacct tgctggcatt taaaatgaat gctcaacagt aaagtgaaca tgacgatcac
     4621 tttagtaaac ggtaccagaa tactggcgat aaacacgatt ccggcgaccg caatgttgct
     4681 acttgccagc gacataatcc cggaaagaat cgtatcttcc tgccggccac cattcagata
     4741 aatgatagaa ataggcaata ggttggctgg taacaacaaa acgattgacg ctaacagcgc
     4801 cgcccagcat ttttgcaaac tatgacgacg acgcaggcgt agcgggatat ggcaacgcgg
     4861 gcagcgacca cgctgatcag gatagccggt aaaatggcac ccaatacaga cacgaagttt
     4921 ctcgtcccta cgcgtagcgg ggcgctgcgg ataatatcgc tcccacagct cctcaacatt
     4981 aagatgtgac aacgtcaccg tcgttaaaat caccaacgcc acaaaagaga acaagccgac
     5041 acccgcctgg atatgggcat aatcctgtac ctttatagaa gcaacgccaa tgccgaccag
     5101 gtagatatcc agcattaccc actctttaag acgctcaagc atcagcagta ccggacgtaa
     5161 attcattccc agtcggttac caaaccataa atatgctatg gaggacacca gaatgagggg
     5221 ggcaccaata acgcagaaaa agaccatcgc ccccgttatc gtatcgccct gtttggtcat
     5281 ttgccagatg ccttgcatga cgttggcgtc gatacgaata cctaacagcc agatatgcaa
     5341 cagcggttcg ccccaggcaa acggcatcag caacagcatg gtgaaagcca ttgccgccag
     5401 gcgcgttagc gaccagtcgc gcccgtcgcg aatttttgcc tgacagcgcg gacaataggc
     5461 actctgatga gaatttatct cgggcaggct aaacagcatg tcacattgcg ggcaacgttg
     5521 gtaatcacca cgcggcagtt cctcgccgat tgccctcact gttatctttt ttgtcggcgt
     5581 aatttgtggt gtgttaagag ccatgacgtg ccaaatgtaa taagaattaa cattaatctt
     5641 aacccatgat gggaatgatc ttgagcatta acgcatttaa tgcttatttt aataggctgg
     5701 cagttccagg taagacaaac aagtgattat ataatgaaca aaacagaatt ttacgcggat
     5761 ttaaatcgcg actttaacgc gctgatggcg ggagaaacca gttttctggc aacgcttgcg
     5821 aacaccagtg cgttgttata cgagcgtctc actgacgtaa actgggcagg tttttatttg
     5881 cttgaggacg atacactggt actcggacca tttcagggca aaattgcctg tgtccggata
     5941 cccgtcgggc gcggggtgtg cggcactgcg gttgcccgca atcaagtgca gcgtatcgag
     6001 gatgttcacg cgtttgacgg gcatattgcc tgtgatgcgg cgagtaattc tgaaattgtt
     6061 ctgccgctgg tggtgaaaaa tcagattatt ggtgttcttg acatcgatag taccgtcttc
     6121 ggtcgcttta cagacgagga cgagcaaggc ttacgtcagc ttgtggcaca gcttgaaaaa
     6181 gtgcttgcaa cgacggatta caaaaaattc tttgcgagcg tcgcaggata atcaacggat
     6241 aacgtagcat ttactggtag cgtcattata atgacgcctg ttcatgcctg cgcttgttgg
     6301 ctacgtccgt tgtaatcagg aaatttcatg gaaaatcaac ctaagttgaa tagcagtaaa
     6361 gaagtaatcg cgtttctggc cgaacgtttt ccccactgtt tcagtgcgga aggtgaagcg
     6421 cgtccgctga aaatcggtat ttttcaggat ttggtcgatc gtgttgccgg ggaaatgaac
     6481 ctgagcaaaa cgcaattacg atccgcttta cgtctctaca cttcgagctg gcgttatctt
     6541 tacggtgtta aacccggcgc aacgcgtgtc gatcttgacg gcaacccatg cggtgagctg
     6601 gacgagcaac atgtagagca tgctcgcaag cagcttgaag aagcgaaagc gcgtgttcag
     6661 gcacagcgtg ctgaacagca agcgaaaaaa cgcgaagctg ccgcagctgc tggtgagaaa
     6721 gaagacgcac cgcgccgcga acgcaagcca cgtccgacca cgccacgccg caaagaaggc
     6781 gctgaacgta aacctcgtgc gcaaaagccg gtagagaaag cgccaaaaac agtaaaagca
     6841 cctcgcgaag aacagcacac cccggtttct gacatttcag ctctgactgt cggacaagcc
     6901 ctgaaggtga aagcgggtca aaacgcgatg gatgccaccg tattagaaat caccaaagat
     6961 ggcgtccgcg tccagctgaa ttcgggtatg tctttgattg tgcgcgcaga acacctggtg
     7021 ttctgaaacg gaggccgggc caggcatgaa catgtttttt aggcttaccg cgttagctgg
     7081 cctgcttgca atagcaggcc agaccttcgc tgtagaagat atcacgcgtg ctgatcaaat
     7141 tccggtatta aaggaagaga cgcagcatgc gacggtaagt gagcgcgtaa cgtcgcgctt
     7201 cacccgttct cattatcgcc agttcgacct cgatcaggca ttttcggcca aaatctttga
     7261 ccgctacctg aatctgctcg attacagcca caacgtgctg ctggcaagcg atgttgaaca
     7321 gttcgcgaaa aagaaaaccg agttaggcga tgaactgcgt tcaggcaaac tcgacgtttt
     7381 ctacgatctc tacaatctgg cgcaaaagcg ccgttttgag cgttaccagt acgctttgtc
     7441 ggtactggaa aagccgatgg atttcaccgg caacgacact tataaccttg accgcagcaa
     7501 agcgccctgg ccgaaaaacg aggctgagtt gaacgcgctg tgggacagta aagtcaaatt
     7561 cgacgagtta agcctgaagc tggcaggaaa aacggataaa gaaattcgtg aaaccctgac
     7621 acgccgctac aaatttgcca ttcgtcgtct ggcgcaaacc aacagcgaag atgttttctc
     7681 gctggcaatg acggcgtttg cgcgtgaaat cgacccgcat accaactatc tttccccgcg
     7741 taataccgaa cagttcaaca ctgaaatgag tttgtcgctg gaaggtattg gcgcagtgct
     7801 gcaaatggat gatgactaca ccgttatcaa ttcgatggtg gcaggtggtc cggcagcgaa
     7861 gagtaaagct atcagcgttg gtgacaaaat tgtcggtgtt ggtcaaacag gcaagccgat
     7921 ggttgacgtg attggctggc gtcttgatga tgtggttgcc ttaattaaag ggccgaaggg
     7981 cagtaaagtt cgtctggaaa ttttacctgc tggtaaaggg accaagaccc gcactgtaac
     8041 attgacccgt gaacgtattc gtctcgaaga ccgcgcggtt aaaatgtcgg tgaagaccgt
     8101 cggtaaagag aaagtcggcg tgctggatat tccgggcttc tatgtgggtt tgacagacga
     8161 tgtcaaagtt caactgcaga aactggaaaa acagaatgtc agcagtgtga tcatcgacct
     8221 gcgtagcaat ggcggtgggg cgttgaccga agcggtatcg ctctcaggtc tgtttattcc
     8281 tgcgggtccc attgttcagg tccgcgataa caacggcaag gttcgtgaag acagcgatac
     8341 cgacgggcag gtgttctata aaggcccgct ggtggtactg gttgaccgtt tcagtgcttc
     8401 ggcttcagaa atctttgccg cggcaatgca ggattacggt cgtgcgctgg ttgtgggtga
     8461 accgacgttt ggtaaaggca ccgttcagca ataccgttca ttgaaccgta tttacgatca
     8521 gatgttacgt cctgaatggc cagcgctggg ttctgtgcag tacacgatcc agaaattcta
     8581 tcgcgttaac ggcggcagta cgcaacgtaa aggcgtaacg ccagacatca tcatgccgac
     8641 gggtaatgaa gaaacggaaa cgggtgagaa attcgaagat aacgcgctgc cgtgggatag
     8701 cattgatgcc gcgacttatg tgaaatcagg cgatttaacg gcctttgggc cggagctgct
     8761 gaaggaacat aatgcgcgta tcgcgaaaga tcctgagttc cagaacatca tgaaggatat
     8821 cgcgcgcttc aacgctatga gggacaagcg caatatcgtt tctctgaact acgctgtgcg
     8881 tgagaaagag aataatgaag atgatgcgac gcgtctggcg cgtttgaacg aacgctttaa
     8941 acgcgaaggt aaaccggagt tgaagaagct ggatgatcta ccgaaagatt accaggagcc
     9001 ggatccttat ctggatgaga cggtgaatat cgcactcgat ctggcgaagc ttgaaaaagc
     9061 cagacccgcg gaacaacccg ctcccgtcaa gtaatatcaa tcaggcacaa gaaattgtgc
     9121 ctgatttttt aacagcggca agatgccgta aatcagatgc tacaaaatgt aaagttgtgt
     9181 ctttctggtg acttacgcac tatccagact tgaaaatagt cgcgtaaccc atacgatgtg
     9241 ggtatcgcat attgcgtttt gttaaactga ggtaaaaaga aaattatgat gcgaatcgcg
     9301 ctcttcctgc taacgaacct ggccgtaatg gtcgttttcg ggctggtact gagcctgaca
     9361 gggatacagt cgagcagcgt tcaggggctg atgatcatgg ccttgctgtt cggttttggt
     9421 ggttccttcg tttcgcttct gatgtccaaa tggatggcat tacgatctgt tggcggggaa
     9481 gtgatcgagc aaccgcgtaa cgaaagggaa cgttggctgg tcaatactgt agcaacccag
     9541 gctcgtcagg cagggatcgc tatgccgcaa gtggctatct accatgcgcc ggacatcaac
     9601 gcttttgcaa ccggtgcgcg ccgtgatgcc tctctggttg ctgtcagcac cggtttgctg
     9661 cagaacatga gcccggatga agccgaggcg gtaattgctc acgaaatcag ccacatcgcc
     9721 aatggtgata tggtcaccat gacgctgatt cagggcgtgg tgaacacctt cgttatcttt
     9781 atttcccgta ttctggcgca gcttgccgcg ggttttatgg gcggaaatcg tgatgaaggt
     9841 gaagagagca acggcaaccc gctgatctac tttgcggttg caacggttct ggaactggtg
     9901 tttggtattc tggcgagcat tatcaccatg tggttctcgc gtcatcgtga attccatgct
     9961 gatgccggtt cggcaaaact ggttggtcgc gagaaaatga ttgccgcgct gcagcgcctg
    10021 aaaaccagct atgaaccgca agaagcaacc agcatgatgg ctttctgcat taacggtaag
    10081 tcgaaatcgc tcagtgagtt gttcatgact cacccgccgc tggataaacg aattgaagct
    10141 ctgcgtacgg gtgaatacct gaagtaatat gaagtaaata ctaaaaagcg cgtcctgatc
    10201 gacgcgcttt ttttatgccc tggatcgtgg ctgagtgata cgtaaaccac tgacacaggc
    10261 agcaatcact gccagaatag ccgcagccat cagcgaaacg tgcgtaccat tatcaccgaa
    10321 ctgatttagc atcagcgcca ccagtgccgc gccgctactc tgacccagta gacgagccgt
    10381 tcctaacatg ccactggctc caccgctacg ttcgcgagga gcggaggtaa taatggtgtg
    10441 gttattgggt gactggaaca agccaaatcc agcgccacat aagatcatcg gccagataat
    10501 attgatatcc gcaggtgacg cgggcagcag aaccagggga aaaagccccg cagccatgat
    10561 gaacaaccct agagcaccca gcaatcctgc atgtacgcgt tcaatcaaat agcctgccaa
    10621 cggagccatc accatcgttg ctaacggcca cggtgtcaga agtaaacctg tttcgacttc
    10681 actacgcccg agcacggttt gcaggtaaaa gggcagggaa accattgcca gcatttgtgc
    10741 gcagaaagag caaacagatg tgcaaataga aagtgaaaac agcgggatac gcagtaaatc
    10801 caccggtagc agcggtacgg gaagagaaag ctggcggcga ataaagaaaa taccaacaac
    10861 aaccattacc accagttccg cagcaattaa cgtcagcgat tgcccctgag cgaaaccact
    10921 caacgcagtg ataagcaggc cgaaggttaa cgcgttcatc acggcgctgg gcaggtcgaa
    10981 acggggttta ctggcgcgag aaccattggg tggcagaaaa cgcatcgcca gaagcagggc
    11041 gataatacct aacggtacgt tgattaaaaa taaccatttc caggatgcga tggagaggat
    11101 tgctgcagca attgtcggcc cggcagcaga agagacggca acaatatggc gattgttggc
    11161 agggcgacgt tggcgattgc gccgtcaagg acagccatcg aaatgccaat cacaatggtt
    11221 aatatcgcac cgtatcgctg gggcaatggc aggccgtcgg cctgaacttt tggcatagga
    11281 attttatatc tttggtgaat aatcaacagg ggtatgattc tagcactgat attcacggtg
    11341 gatgtcgcag aattgtaact aaccggcaaa agatggattg cgaccaccct ggtgcgaaat
    11401 tataataaaa aacagttctg atttttataa aacactcgca atgaggtgat aaatggctaa
    11461 cgcagatctg gataaacagc ctgattctgt atcttccgtg ctaaaagttt ttggcatttt
    11521 gcaggcgctg ggtgaagagc gcgaaatagg gataaccgag ctgtcgcagc gcgtcatgat
    11581 gtcaaaaagc accgtttatc gctttttaca gaccatgaaa accttaggtt atgtggcgca
    11641 ggaaggggag tcggagaaat attccctgac cctgaaattg tttgaactgg gcgctcgcgc
    11701 gttacaaaac gtcgatttaa ttcgtagcgc agatatccag atgcgtgaga tctcccgcct
    11761 gaccaaagaa actatccacc tcggcgcact ggacgaagac agtattgttt acattcacaa
    11821 aattgactct atgtacaatt tgcgcatgta ttcacggatt gggcgtcgta atccgctgta
    11881 cagcaccgcg attggtaagg tactgctggc atggcgcgat ctcgatgaag tgaagcaaat
    11941 tcttgagggc gtggagtata aacgcagtac cgagcggacc atcaccagta cagaagcgtt
    12001 attacccgtt ctggaccagg tgcgcgagca ggggtatggc gaagataacg aagagcagga
    12061 agaagggctg cgatgcattg cggtaccggt atttgatcgc tttggcgtgg tcattgccgg
    12121 tttgagcatc tccttcccga cgttgcgttt ctctgaagag cgtttacagg aatatgtcgc
    12181 aatgttgcat accgcagcgc gcaaaatttc tgcccaaatg ggttatcacg actatccgtt
    12241 ctgatgagag taagaacctg tcggaatatc aaacagacag gttctttatt tagcatgaga
    12301 aaaataaagt tgaaggtgcg ttatattaaa cgcgcttgct ataagaatat tttactcatg
    12361 agtgagaatc tggttgttta ttacccttaa ccattatcga ccacgatatt gcttttacgt
    12421 aacagtgggc aatctgttat ccccaagaaa ccacttttag tgtgcaagta ttgtaccgtg
    12481 ctggtgcctc tggcagtcag ataggtacat tgcaaaccta atcctgcggc attctctttg
    12541 cttccaatca aaacgccata tccgctgagt aataatccta tccataccag tgctatcagc
    12601 ataactgtgc gaatgatgaa tcgcattaca acctcttctc tttttatgtt cgcttaatcg
    12661 tagcggcaat atgcgctgaa gcaagctact cattccgaaa aagcacgaat atcgacatag
    12721 ttaggcgctg tttaactaac gcatgctagt ttaatgacat aaggtaggtg aagcggagat
    12781 tggagtgaaa aagtttcgat gggtcgttct ggttgtcgtg gtgttggctt gcttgctgct
    12841 ttgggcgcag gtattcaaca tgatgtgcga tcaggatgta caatttttca gcggaatttg
    12901 tgccattaac cagtttatcc cgtggtgata tcattttttc agcactagat tttcttcctc
    12961 tccagcagtg gtagaatagc gcctctactc agaggtggtg aaatgaacga agttgtaaat
    13021 tcaggcgtga tgaacattgc gtctttggtt gtatcggtgg tggttcttct tatcgggctc
    13081 atcttgtggt tttttatcaa tcgtgccagt tcacggacta acgaacagat tgaactgctt
    13141 gaggcgttgc tggatcagca aaaacgtcaa aatgcactgt tacgtcgttt gtgcgaagca
    13201 aacgaaccag agaaagcaga taaaaagacc attgagagtc aaaaatcggt tgatgacgaa
    13261 gatattattc gcctggtcgc cgaacgataa tccttcctca atcttgagta accccgtcgt
    13321 tgaagggtta ctcaattcat cctgattata cgcactgtca tggtgcaact gcttcaaatt
    13381 aaaactctgg tctgacttca aataattatc atattattta tttgcgtaac gggatatcag
    13441 aaaaagatat tttcctcatt aatttattag gatgtttaca tcggatttgt gattaagcgt
    13501 ggtattattt attagcgaaa cgtttctctc ttgatttttt tgctcattca tcaatttttc
    13561 ttattttaaa ttttcaatcc ctttggggat tgacttctct ttagggtaat tgatagccgt
    13621 taaccgactg ttttatgaga aaaagtgata taacttttta ttcattgcat agcaaaaaat
    13681 gtgatattgc acgcactatg taataacttc tcccactggc ctggaacaac tgaacttatt
    13741 gaactatgtt agaaaatacg ccagtttaag tatctgcctg aactggcaag gttaagcaca
    13801 atgatatatc ggcgcgtatt ccgttgcata agtgtgcaaa aaaagtggaa gacgtatcga
    13861 gatttgtgcg tctgatcgag acatgtttaa aaatggcttg ccataattaa cgttgtatgt
    13921 gataacagat ttcgggttaa acgaggtaca gttctgttta tgtgtggcat tttcagtaaa
    13981 gaagtcctga gtaaacacgt tgacgttgaa taccgcttct ctgccgagcc ttatattggt
    14041 gcctcatgca gtaatgtgtc agttttatct atgttatgcc tgcgggcgaa gaaaacaatc
    14101 taaggaattt ttcaaatggc aaagattaaa ggtcaggtta agtggttcaa cgagtctaaa
    14161 ggttttggct tcattactcc ggctgatggc agcaaagacg tgttcgtaca cttctccgct
    14221 atccagggta atggcttcaa aactctggct gaaggtcaga acgttgagtt cgaaattcag
    14281 gacggccaga aaggtccggc agctgttaac gtaacagcta tctgatcgaa tccactgatc
    14341 tgaagtgtga atacgcttca atctcgctat aaagcctcgt cgaatgcgag gctttttact
    14401 atgctttatc ttcgctcctg gcgttcggat atttgcccgc cgcgtgattc gcgttacact
    14461 tgcggccttt agtatcctgc cggagttgtc atgtcttttt cctgtccact ttgccatcag
    14521 cctctttcgc gtgaaaaaaa cagctatatc tgtccccagc gacatcagtt tgatatggcg
    14581 aaagaagggt atgtcaatct gctgtccgtt cagcataaac ggtctcgtga tccgggcgac
    14641 agcgcggaaa tgatgcaagc acgccgcgca ttcttagatg ccggacatta tcagccgctg
    14701 cgtgatgcaa ttgtcgccca actgagggaa cggcttgatg ataaggccac ggcggtgctg
    14761 gatattggct gtggtgaagg gtattacaca cacgcatttg ccgatgcgtt gcccgaaatc
    14821 accacgtttg gtctggatgt ttcgaaggta gcgataaaag cggcggcgaa acgctatccg
    14881 caggtcactt tttgtgtcgc ttccagccac cgtttgccgt tttccgatac cagtatggac
    14941 gccataatac gtatttacgc gccgtgtaaa gcagaagaat tagcacgagt agtgaagccc
    15001 ggcggctggg tcattactgc cacgccggga ccgcgacatt tgatggagct gaaggggctg
    15061 atttacaatg aagtacatct tcatgcacct catgcagaac aactggaagg ttttacatta
    15121 cagcagagtg cggagttgtg ttatccgatg cgtcttcgcg gtgatgaagc cgtcgcatta
    15181 ttgcagatga cgccgtttac ctggcgtgcg aagccagaag tctggcaaac actggcagca
    15241 aaagaagtgt tcgactgcca gacggacttt aatattcacc tctggcagcg ttcttattaa
    15301 ccgtggaagt gcgtccagag gatctggacg ccgatgccga tcagtaccag cccgccgaga
    15361 atttccgctt ttttcccaat aattgagccg ataaagcgac caaccatcat ccctaatgtt
    15421 gacataatca aggttgcaca accaatggcc aatgcggtcg cgataatgtt gacctgcagg
    15481 aaagcaagac caacacccac agccatggca tccaggctgg tggcaatcgc ggtggttacc
    15541 agtagccaga aaccgtgtcg acggcgcggc tcttcatctt catcatctgc gccacgaaaa
    15601 ccctcaataa tcattcgccc gccgaggaat atcagcagca caaacgcaat ccagtggttc
    15661 cattcaagga caaaccggct ggctaacatg cccattcccc agccgatcag cggcgtcagg
    15721 gtttcgacgg caccaaaaat aaggccggtt cgcaatgctt cagaaaattt cggtttatgg
    15781 agggtggcac ctttaccgat tgatgcagca aatgcatcca tcgacatacc aaacgcaaga
    15841 agaacagtag cagtgatatt catgacaatg tcctgaccgg ggtatccata aaacacatca
    15901 tcgcccccag caaacatgca tacatgaaca acaagccgtg atggttttcg cgttcatgca
    15961 ggcatagcga ttgatgtgca tatggtctcg cctgactgaa agtattcagt ccgtacgtgc
    16021 cacgttttgc aacgagtatg ttgacacgta catttccgga atctggaaat cggctactcc
    16081 ccaaatgagg gcgcaacctt aacatatttt gagaatataa aacaactgtt ggaatatatt
    16141 atttcattgc tttattgata acgattttca tttggattta tatatcaatg aaacgttaaa
    16201 ataaataaca aataagggaa gaggtaatat agcctaagct atgtctgatt attaaaaata
    16261 cactaaacat agctttggct aaattcataa cttattgagt tgaaacgaga agcttataaa
    16321 ttttttccag atcgtcgata tttcgaacgc ggattaacag ccgacgctgt tctaattgca
    16381 tcaccaacac gccatcttcc gacaagttca tcgctttgat tcggctatat tcaatccaga
    16441 cattggcgaa gaaaaaacct ttttgtttaa agatgatctt cggaacgcgg atccagaata
    16501 tataaaaacc catcagagcc agtgcgctta ataaccatgt cgttattaac gcaccatgat
    16561 tcgtgacgtt gttatagata agaatgacaa tcaatccgac gaagataacg ctatcgatgc
    16621 gaccacgccg gagcaaagga attgccagca gggtggggcc gttacggcgg ggcatgatga
    16681 actgatcgta gatcgcgaag gccaggagtg cggcgatgaa aagaatcagt accaggtccg
    16741 tgattgtcat taatcctcca gataaaaaaa cggggccaaa aggccccggt agtgtacaac
    16801 agtcttacag tcccagcagg ccgcaagcgt aaccagcgat accgatgacg aagaagccaa
    16861 cgatgatcca cagcgggtta acttttttgc gcagtagcca catacaagca aaggtcagca
    16921 gcagtggtac caggcctggc attaactggt ccagaatagt ctggacagta gtaacgtgtt
    16981 ctttgcccgt ctggtcagta atgcgagaga caaccagcgg gatgttgaca tgtgtccact
    17041 tgttaaccaa tgcccccatg acaaacaggc cgaggataga cgccccttcc gtcagttttt
    17101 gcaggaagcc accacccata tctttaacga tatcgatacc tttggagtaa ccatacgcta
    17161 cgccgtagta acgggttgcc agacgcacca ggttaaacag gatgaagaac agcagcggac
    17221 ctaacaggct gccgctcatc gcgataccgg cacccagtgc tgcaaatacc ggacgtacgg
    17281 ttccccagaa gatcgggtcg cctacaccag ccagcggccc catcaaaccg actttgatac
    17341 cgttgatagc accgtcgtcg atctctgcgc cattagcacg ctgttcttcc agcgccaggg
    17401 ttacgccgag aatcggcgca gccacgaacg gctgggtgtt aaagaactcc aggtgacggc
    17461 gaatagcttg tttacgagct tcgttgttct cagggtagag gcgacgaatt gccggtacca
    17521 tagagaagca gaaacccagt gcctgcatac gttcgaagtt ccatgaaccc tggaagaggt
    17581 tagaacgcag gaagacgcca cgaatatcac tttgagtgag ttttttctcg gtggtagttt
    17641 gagttgtatc aaccatttcg ctcacctgtt agtccagttc gttatcgaga tcgttgttac
    17701 cagctgcctg agcaggcgca ccggctacgc ggttgtattt cgggctaagt tggatgtaga
    17761 gcactgccat aacagtacca atcacaccca gagcaaccag gttaaagttg gtgaatgctg
    17821 cggttacgaa gccgaggtag aagaacggca tcaggtagcc agcacgcatc atgttgataa
    17881 ccatcgcata accaaccacc acgatcatgc caccggcgat attcagacca ttggtcacca
    17941 cttccggaat cgcattcagc atgttctgta cttcgctggt accaacagac agcgcaacga
    18001 tgacggccgg aatagccaca cgcattgctt gcaggaacag agaagaaacg tggatccagg
    18061 aaatcgctgt caggttgccg ttatcagcag ccttatccgc agcgtgctgg aaagcaacgg
    18121 taatagtacg aacgatgatg gtcagtacct ggcccgcagc ggccagaggg attgccagtg
    18181 cgatacctgc accaatgctc tgatgacctg cgataaccag aatggtagaa atgatagaag
    18241 ccagagcggc gtcaggcgca actgcagcac cgatgttcat ccagcccagc gcgatcattt
    18301 ccagcgtacc accgataata ataccggttt tcatatcccc aagaacgata cccaccaggg
    18361 tacacgcgat tagcggacgg tgaaactgaa attcatcgag gattgatccc atacctgcga
    18421 tacaggctac gataaatacc agcacaattt gaagagtggt aatctccatt gtacttctcc
    18481 tgtttacgta agacttaagt gtgaaaactg agtgataatc aacacaatac gttacttatc
    18541 gattttgctg atcagatcca tcattttcag tttcggatcg gtggaaacct tacggacttc
    18601 cagctcaata ccgcgcgcat tcagtttctt gaacgcctca atatcttttt catcaaccga
    18661 aaccgcgtta ttcacctggg ttttaccctg acggaatgcc ataccaccga cgttaacaga
    18721 ggtgattttc acgccgcctt caacgagacg ctctacatct gttgggttgg taaataacag
    18781 cattacgcgt tcgccagcat atttcgggtt gttgtagacg cgaatcattt tggcaacatc
    18841 aactacgtgt gctgttacgc ccggaggtgc aacctgggtg agcagtgtct tacgaacggt
    18901 atccgcagcc acttcatcac taacaacaat aatacgggag acattggttt ctttggtcca
    18961 gcgggtggcg acctgaccgt gaatcagacg gtcgtcgata cgcgcaaggc caataaccat
    19021 gtagtcgttt ggccccattg gttttgccgg agttggagcc gcttttggtg ctgcggcagc
    19081 gggtgctggc gcggcttttt caaccggttt ggctttcagt gctttcacgc cttcacggcc
    19141 tgtttctact gccagcgcaa ccagttcatc aaagcttggg tcatcatcac gagccattaa
    19201 cgtttccacg agcattggaa tgttaacgcc tgcaatgact tcataatgct ctttgtcgac
    19261 gacaatgcgg ctggcagcat tgaacgggct gcctccccat gtatcaacga gaaacagcac
    19321 gcctttagtg gtgtcgagtt ttgccaactg agcgttgtac ttttcaatca gcgtttcggc
    19381 attttcacct ggaacgaaat cgatccagcc gacgttttcc tgctcgccta acagcatttc
    19441 tgccgtttta agcaactgct ctgcagccca accatgtgtg cctataacaa tagcaatggt
    19501 cacttgctac ctcctttatt atcgttaaca cctcaacgtg ccagatgtat ttttgaatcg
    19561 tcgtccacaa tcgaatcgat tcagataagg gctacaacca aaaacccctg cgtttcgcga
    19621 tttattttag atatcgaaaa aattatttta tgtgatgaag atccgtaatt taactttcga
    19681 ttagcagaaa tttcgaaagg taaaatatcc ttgtcacatt cgtttgcaaa ggaaggtaaa
    19741 tctttgccaa atcagaggcg tctctgatat gtttaactcc cgtttaacaa ccatggagta
    19801 tagggcagta gcccgcagta tggatcgtca ccgacgtcat ttcagcatca ggccttttaa
    19861 cgcctgcctt tctggcactc tatgccgcac ctttcgtttg cattttgtcg ttacgcctgc
    19921 attatttctg gcgtcgaata gctattcctt aagcaggagc ttgtcatgga attcttaatg
    19981 gacccctcaa tttgggcggg gctactcacg cttgttgttc tcgaaattgt gctgggtatc
    20041 gataacctgg tcttcatcgc cattcttgct gacaaactgc cgccaaaaca acgcgataaa
    20101 gcgcgtttgc tggggttatc actggcgctg attatgcgtc tggggctgct gtcgctgatt
    20161 tcatggatgg tcacgctgac caaaccgcta tttaccgtca tggatttctc cttctccgga
    20221 cgcgacctga ttatgttgtt cggggggata ttcttgctgt tcaaagcaac aaccgaactg
    20281 catgaacggc tggaaaaccg cgatcatgat tccggccacg gtaaaggcta cgccagtttc
    20341 tgggtggtcg tcacacagat tgtcatcctt gacgccgtct tctcgttgga tgcggtaatt
    20401 actgcagtag ggatggttaa ccatctgccg gtgatgatgg cggcggtagt gattgcgatg
    20461 gcggttatgt tgctggcatc caaaccgctg acgcgattcg ttaaccagca ccccacggtg
    20521 gtggtgctct gtctgagctt cctgttaatg attggtctga gtctggtggc agaaggtttc
    20581 ggtttccaca ttccgaaagg ttacctgtat gccgcgattg gcttctcgat catcatcgaa
    20641 gtgtttaacc agattgcgcg tcgcaacttt attcgccacc agtcgacttt gccgctgcga
    20701 gcgcgtactg ccgatgccat cctgcgtttg atgggcggga aacgtcaggc caatgttcag
    20761 cacgatgccg ataacccgat gccgatgccg atcccggaag gtgcatttgc cgaagaagaa
    20821 cgttacatga ttaacggcgt actgacgctt gcgtcgcgtt ctctgcgcgg gatcatgacg
    20881 ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat ccgcgagcaa
    20941 ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga tgaaatcatc
    21001 ggtattgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga tgtggcggcg
    21061 attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat caacctgttg
    21121 ggcgtgctgc gtcgtgctcg cgggagcttt gttatcgtga ccaacgagtt tggtgtggta
    21181 caaggtctgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt cccggacgct
    21241 gacgaaacgc cggaaatcat tactgatggt gacggctggc tggtaaaagg cggtacagat
    21301 ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga tgatatcgcg
    21361 acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg cgatgtgatt
    21421 gatgtagggc cactgcatat caccatcatt gaagccaatg attatcgtgt tgatctggtt
    21481 cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcattaac gtaacggcat
    21541 aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg ccagccattt
    21601 gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa tatgcacgcc
    21661 atgacggcgc aaatacttcg cctgttcttg cgtttccacg ccctccgcca ccagttcaat
    21721 attcagtctt tgccccagcg cgatgatgat atcggtcacc gtcgagttaa ccgcgtcaga
    21781 acctatagct gcggtaaatg acttatcaat tttcagcatg tcaggacgta atgtttcaag
    21841 ccaggaaaat gaactgttgc cggtgccgaa gtcatcaatc gccagtttga cgtttttacg
    21901 atgtagctcg cgggcaatcc gataatcaac atccagtaag gcatcgcgtt cggtgatttc
    21961 gaggatcagt tgctgaatcg ggtgagcgct aaaccagtac tgattgagat cttttatcag
    22021 cacaccacgg cgaaaatggc tgggtgcgac gttaatgcca acatgaaact gactactcat
    22081 cgggaaaaca tggcgctgac gaatggtttc tgccatcaca tagcgggtca gtggcacaat
    22141 taaatgatgt tcttccgcga taggaataaa cacatccggt gaaatccagc cctgacgcgg
    22201 attgttccag cgcagcaaaa tctctacacc aatacattgc tggctgcgcg cattaagcaa
    22261 aggctgacag aacaattcga actcatgttg cgccaggccc agattgattt cgcgggaaaa
    22321 gctcatccgg taagcggtcg ccagccaggc gatgtagccc accagtaaac ttagcagcac
    22381 cgccagcggt aattgtgttg gaagatagtg ccatgccagc gccgtagccc ccggaccatt
    22441 aacgttaatg gtaaaaggaa agcgttgcga agaaacctga tagttttcat tgtcttcagg
    22501 ttgcggaagg gaatctacca gcccattacc atagagcaaa tgccgtttgt ccaccgtcag
    22561 gctggcgcta ctgatttgcg gcagttgtgg ctcaaggagc attgccgtca gtaagtcgat
    22621 gttaatcatc tccatgaccc cagcattgct actgcccgct gcaggcgtcc attgaatcaa
    22681 aaccggactg cctttaatca gggcacggtc gatcgtcagg cgtaaaagtg gttgcggtgc
    22741 aggaagttca gccagaatgt ccacgacggg cacattgcga taaccaaaaa tgctggagca
    22801 atagagtgtt ccgtcttgca ccaggccaat ggatcgcaca gtttggagtt ttgccgcctg
    22861 tttacgtaat ggcaaatggg cgacagagca gggcagacca atcagcggaa gcaagacttc
    22921 gctacctgcc tgtaggggaa gcagtacttt atctaattcc tctacagcgt gattggcgaa
    22981 ttgtactacc cgttgttgat ttaagttacg ctgtgaaata aatcgcacgc tcagggtcga
    23041 agcgagcgta acgagtgcgc aaatcgtaca aacaatcatt cgattacggc gataggtttt
    23101 aatgatccgt tgtgcttttt gcatgcgggc caccctgtaa gcgtatggcg acaaacgcct
    23161 gacgcaacag tggaagtgta gtggggaata ttacagcaga cgagaaagcg ggtataaatt
    23221 cgcccatccg ttgcagatgg gcgagtaaga agtattagtc acactggact ttgattgcca
    23281 gaccaccgcg tgaggtttcg cggtatttgg cgttcatgtc cttaccagtt tcgtacatcg
    23341 tttcgatgac cttatccagc gagacgcgcg gtgcactggt gcggcgcaga gccatccgtg
    23401 cggcgttaat cgccttcaca gaggcaatgg cattacgctc aatgcacggc acctgaacct
    23461 gccctgcaac cgggtcgcag gttaaaccaa ggttgtgttc catgccaatt tccgccgcca
    23521 cgcaaacctg ttccgggcta ccgcccagca gttctgcaag acccgcagca gccattgaac
    23581 aggcaacacc cacttcgccc tggcaaccaa cttccgcacc ggaaatagag gcgttcattt
    23641 tatacaatgc accaatcgcg cccgctgcca taaagtaacg ggtatagatg tccgggctga
    23701 ccgattcaat aaagtggtca tagtaagcca gcactgccgg aacgataccg caggcaccgt
    23761 tggttggcgc agttaccaca cgaccaccgg cggcgttttc ttcgttaact gccagcgcaa
    23821 acatgtttac ccagtcaatg acattcatcg gatcgttaga cagtttatcg ctggaaacta
    23881 acatccggcg cagggcagac gcacgacgtg gcacgcgcag cgggcctggc agtacacctt
    23941 cggtgttcat cccgcgatcg atacatgcct gcatggtttg ccagacatgc gcgaaatact
    24001 cgtcgatctc tttcttgctg tgcagcgcca gttcgttctg catagcgaga ccagacagcg
    24061 aatagccggt ttcattacag tacgcgagca gttcggtggc agatttgaac ggatacggca
    24121 cgctcacttc gttggcagca tcctgaccaa agtgttcttc atcgacgata aaaccgccgc
    24181 cgatggaata ataagttttg ctgtagacga cttcatcgcc gttataggcg tggatttgca
    24241 taccgttttc atgcagcggc aggttgccgt tatgaaaacg catcccgttg tcgcgcggga
    24301 aatccacttc atgccgtccc tgtgccagca gcagacgttc gcgctcttct acgtcgcgaa
    24361 taaaaccggg aatactgtcg atatccacgg tggcaggttc gttacctgca agacccataa
    24421 taatggcgat atcggtgtgg tggcctttac ccgtcagcga cagtgaacca taaacgtcca
    24481 cggcaacgcg agtaacgcta tccagtaagc ctttttcgac cagatcatcg acgaactgtt
    24541 tacctgcctt cataggccct acggtatggg aagatgaggg accaatcccc accttaaaca
    24601 tgtcgaatag actaatcacg ataatactcc tgacaaggtg actggacttc cagtaacgaa
    24661 ctaataacag cgcatagtgt aagagggaac gcggcgactg gcttaactat tcacatgaat
    24721 taaactaatc aaaaaccgcg atttaactaa tattgcgatg attgtctcaa gtagtgtttt
    24781 gtagatgtaa agataagtat agtcagggtt tcacaccaat ttgcagcgcc agctcacgaa
    24841 ttatgcctgc ggtcattccc catacaaaat actgttcgta ccaggacagc catacccgat
    24901 gtgaatcgcc acggcggtag atatctaaag ggtgataacg acccagatgt aatgcctggg
    24961 cgagcggcat ttcaaacacc gccgagactt catcttcact ggcgcgatac ggcagatcgg
    25021 gcgggataat gccgaccact ggggttacct gatagccagt tacgctatcg acgggcggca
    25081 gcacgccgat aacttcaacg gcggaaggcg gtatagcgac ctcttcttca gcttcgcgca
    25141 gcgcggcggc gataactgat gcgtccgtgt catcgactgc acctccaggg aatgccactt
    25201 gtccagcgtg tttacgcaga tgaattgaac gctgagtcag caacaacccc ggttgcggtc
    25261 gacggacgat ggggattaac acggcagcct gacgatgatt tagggtttcc cggttaattt
    25321 gcgggcgcaa aagttgaaag cgcgataaaa aatcatcaag catcaggcta cggtattcca
    25381 cgtcttactt ctccagttgg cgtaatatct tattaacttt atcaaaagtt tcctgatatt
    25441 ccgcttcttc ctggctatcg gcgacaattc caccgcccgc agagcagtat atttgtccgt
    25501 taatggcagt cagagtgcgg atagtaatgc tggtatccat gttgccgcaa aagctcaaat
    25561 agccaatgct gccgcaccag gcattacgtc gctgcggttc cagttcgtcg ataatttcca
    25621 tagcccgtac tttcggagcc ccggttattg agccaccagg aaaagctgcg cgcagcagat
    25681 cgctggcgtg taactgttct ggtagttgtg ccgttatggt gctgaccaga tgatgcacgg
    25741 cagggaaggg ttccaccacg aacagctctg gtacttttac cgaacctgct acggcaacac
    25801 gaccgatatc attacgcatt aaatcgacaa tcatcagatt ttcggcacga tctttcgctg
    25861 agttcgccag tttttctgct tgtttgctat cttcctgagg atcgggcagg cgtggtagcg
    25921 tgcctttaat agggcgggtc tggatttcac tattatcaca aagaataaac cgctctggcg
    25981 aaaggcttaa aattgcaccc tgttcaagac gtaaaaaagc gctaaatggc gcgcggttgg
    26041 cctgattaag ctgaaggaat gcctgccatt catcgccaga ataggtcgca tgaaaacgct
    26101 gggcgagatt tacctgatag caatcaccgc tgtgcagata ttcctgtacc tggcgaaatt
    26161 tttcgccgta ctgctcgcgg gtcatattgg attgccagtc ggaagtaagc gtgaaatctt
    26221 cctgcggcga gaattgctgg ctttccagcc aggcccgacg ggcattgaca tcattatgac
    26281 tcagcaaaga aactgtatga cgctggtggt cgacaatgag cgcccaatcg tagataccca
    26341 ctgccatatc cggcagaacg atatcttgtt ccgcaatttc tggcagtgac tcaaaacggc
    26401 ggcccagatc gtagccaaac aaccccagtg cgccgccctg aaatggcaaa tcttcgttat
    26461 gcgttgggcg aatgtctgcg cgatccagca cctgctggag cacctgtagc gggtcatcag
    26521 tggtcgttgt gcgtttttcg ctttcactaa caacggtttc tttacctaaa gtggttaaag
    26581 tgcaaatcgg atcggcgacc acaatatcaa agcggctata cggatgatcg gcatagccgg
    26641 agtgtaaaag catcgcccac ggcaggtggc ttaagcggga gaaataaaat tcagcggcgt
    26701 cctgacgcca gggtaaagta atcacagcgg gagataacgt cttcatcagt cctgactcta
    26761 ctggctatgt gccgggtagc atgaaataat tagcgccaac aatttagcag gagttaacaa
    26821 tgtttgcagg tttaccttca ctcacccatg aacagcagca aaaagctgtc gagcggatcc
    26881 aggaactgat ggcacaaggg atgagcagcg gccaggcaat cgcgttggtg gcagaagagc
    26941 tgcgtgccaa ccattccggc gagcgaatcg tggcgcgttt tgaggatgaa gacgagtaac
    27001 acactaatcc gtattgattt cgttatacaa gccgatgatt atttgattgt gagaaaaagg
    27061 acgcttttta tatgtgatta aaagcgtcct gatccattta tgctgatgta aattttactc
    27121 agaccagtgc ttttagttct tcctgcatat atgttgaaaa gtattcagga tttttaatca
    27181 cagagcgttc acctgactcc attttttttg cccatccaac cattttttcc gtaaacccag
    27241 ggttccagcc ttcctggtca gctcgcgccg taatatcagc ggcactcgca ggtactccca
    27301 gttcttttaa atatttaaag attcccttcg ccgtgctttc attcatcgaa cttggtgttg
    27361 gcgctgatac atccatggca ccaatgaaat ccagggcttt atctattaca gcaggcatat
    27421 tcttatcctt aaaattaacc atgttatcga taaggctata tatgacct
//
LOCUS       DAZPLI010000044        27105 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZPLI010000044 DAZPLI010000000
VERSION     DAZPLI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27105
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.044"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..921
                     /locus_tag="ACHUU3_001779"
     CDS             <1..921
                     /locus_tag="ACHUU3_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2081866.1"
                     /translation="SATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLN
                     DSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVN
                     IGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLT
                     APQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQM
                     RIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTV
                     NMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="ACHUU3_001780"
     CDS             complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="ACHUU3_001780"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR2081867.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1888..3924
                     /gene="lpoA"
                     /locus_tag="ACHUU3_001781"
     CDS             1888..3924
                     /gene="lpoA"
                     /locus_tag="ACHUU3_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR2081868.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3882..4277
                     /locus_tag="ACHUU3_001782"
     CDS             3882..4277
                     /locus_tag="ACHUU3_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR2081869.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4297..4887
                     /gene="diaA"
                     /locus_tag="ACHUU3_001783"
     CDS             4297..4887
                     /gene="diaA"
                     /locus_tag="ACHUU3_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR2081870.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4897..5472
                     /gene="dolP"
                     /locus_tag="ACHUU3_001784"
     CDS             4897..5472
                     /gene="dolP"
                     /locus_tag="ACHUU3_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR2081871.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5586..6626)
                     /locus_tag="ACHUU3_001785"
     CDS             complement(5586..6626)
                     /locus_tag="ACHUU3_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR2081872.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6699..7334)
                     /locus_tag="ACHUU3_001786"
     CDS             complement(6699..7334)
                     /locus_tag="ACHUU3_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR2081873.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7462..7980
                     /gene="yhbO"
                     /locus_tag="ACHUU3_001787"
     CDS             7462..7980
                     /gene="yhbO"
                     /locus_tag="ACHUU3_001787"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR2081874.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7960..8403)
                     /locus_tag="ACHUU3_001788"
     CDS             complement(7960..8403)
                     /locus_tag="ACHUU3_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR2081875.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8454..8756
                     /gene="yhbQ"
                     /locus_tag="ACHUU3_001789"
     CDS             8454..8756
                     /gene="yhbQ"
                     /locus_tag="ACHUU3_001789"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR2081876.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8743..9246)
                     /locus_tag="ACHUU3_001790"
     CDS             complement(8743..9246)
                     /locus_tag="ACHUU3_001790"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR2081877.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9240..9764)
                     /locus_tag="ACHUU3_001791"
     CDS             complement(9240..9764)
                     /locus_tag="ACHUU3_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR2081878.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9973..10968
                     /locus_tag="ACHUU3_001792"
     CDS             9973..10968
                     /locus_tag="ACHUU3_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR2081879.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10977..11855
                     /locus_tag="ACHUU3_001793"
     CDS             10977..11855
                     /locus_tag="ACHUU3_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR2081880.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDKLGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12061..13068
                     /locus_tag="ACHUU3_001794"
     CDS             12061..13068
                     /locus_tag="ACHUU3_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFR2081881.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13186..14430)
                     /gene="mtr"
                     /locus_tag="ACHUU3_001795"
     CDS             complement(13186..14430)
                     /gene="mtr"
                     /locus_tag="ACHUU3_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR2081882.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14584..16473)
                     /gene="deaD"
                     /locus_tag="ACHUU3_001796"
     CDS             complement(14584..16473)
                     /gene="deaD"
                     /locus_tag="ACHUU3_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR2081883.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAHPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16466..16546)
                     /gene="yrbN"
                     /locus_tag="ACHUU3_001797"
     CDS             complement(16466..16546)
                     /gene="yrbN"
                     /locus_tag="ACHUU3_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR2081884.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16653..17537)
                     /gene="nlpI"
                     /locus_tag="ACHUU3_001798"
     CDS             complement(16653..17537)
                     /gene="nlpI"
                     /locus_tag="ACHUU3_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR2081885.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17646..19781)
                     /gene="pnp"
                     /locus_tag="ACHUU3_001799"
     CDS             complement(17646..19781)
                     /gene="pnp"
                     /locus_tag="ACHUU3_001799"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR2081886.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20028..20297)
                     /gene="rpsO"
                     /locus_tag="ACHUU3_001800"
     CDS             complement(20028..20297)
                     /gene="rpsO"
                     /locus_tag="ACHUU3_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR2081887.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20446..21390)
                     /gene="truB"
                     /locus_tag="ACHUU3_001801"
     CDS             complement(20446..21390)
                     /gene="truB"
                     /locus_tag="ACHUU3_001801"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR2081888.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21390..21791)
                     /gene="rbfA"
                     /locus_tag="ACHUU3_001802"
     CDS             complement(21390..21791)
                     /gene="rbfA"
                     /locus_tag="ACHUU3_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR2081889.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21955..24627)
                     /gene="infB"
                     /locus_tag="ACHUU3_001803"
     CDS             complement(21955..24627)
                     /gene="infB"
                     /locus_tag="ACHUU3_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR2081890.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24652..26139)
                     /gene="nusA"
                     /locus_tag="ACHUU3_001804"
     CDS             complement(24652..26139)
                     /gene="nusA"
                     /locus_tag="ACHUU3_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR2081891.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26167..26619)
                     /gene="rimP"
                     /locus_tag="ACHUU3_001805"
     CDS             complement(26167..26619)
                     /gene="rimP"
                     /locus_tag="ACHUU3_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR2081892.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26826..26902)
                     /locus_tag="ACHUU3_001806"
     tRNA            complement(26826..26902)
                     /locus_tag="ACHUU3_001806"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26866..26868),aa:Met,seq:cat)
BASE COUNT         6423 a         7515 c         6810 g         6357 t
ORIGIN      
        1 tctgcaacgc taagtagcac taactggcaa tacgcctgtt cctgctctgc gggtaaggca
       61 gttaaacttg tctatatggt cagccccgta cttaccacca ctggacatca gacaggatat
      121 tacaaactca atgacagcct ggatattaaa accacattac aggcaaacga cattccagga
      181 ctcacaaccg accaggttgt ctctgttaac acccgattca cacagataaa aagcagcacc
      241 gtatattctg ctgcaaccca aacgggtgtt tgccagggtg acacgtctcg ttatggaccc
      301 gttaatattg gtgcaaatac cacctttacc ctgtatgtca ccaagccatt tctcggctcg
      361 atgaccattc cgaaaacgga tattgccgtc attaaaggcg cgtgggtcga tggaatggga
      421 agcccgtcta caggtgactt ccatgattta gtcaagttat cgattcaggg aaatctcacc
      481 gccccacagt cgtgcaaaat taatcagggc gatgttatta aggttaattt tggattcatc
      541 aatggtcaga agtttaccac ccgcaatgcc atgccagacg gttttactcc agtagacttt
      601 gatatcactt atgactgtgg tgatacttca aagattaaaa actcgttgca aatgcgcatc
      661 gacggtacaa ctggggtagt agaccagtac aacctggtcg ccaggcgaag aagttcagac
      721 aatgtgcccg atgtcggtat tcgtattgaa aatctcggcg gcggagttgc aaatattcct
      781 tttcagaacg gtatccttcc cgttgatcct tccgggcatg gcaccgtcaa tatgcgcgcc
      841 tggccagtta atctggtcgg tggtgagctg gaaacaggaa aatttcaggg caccgccacc
      901 attaccgtca tggtgcggta aaagaagacg ttgcgttttg attaacgcaa cgcttcggca
      961 ctgttacccc tgctgctcca gcgcatactt atacagcgca tttttcttca cgccatgaat
     1021 ttctgcggcc agcgccgccg cttttttcag cggcagttct gcctgtagca gcgccagcgt
     1081 gcgcagggca tcggcgggta agtcttcttc ctgtgcttta tgaccttcga caatcagcac
     1141 catttcgcct ttgcgacggt tttcatcttc ctttacccac gccagcagct cgccaacggg
     1201 cgcgccgtga atggtttccc aggttttggt cagctcacgc gccagaacca cgtagcggga
     1261 ttcgcctaat accgcaacga tatcttccag gctatctaac agacggtggg tagattcata
     1321 aaaaatcagc gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc ggccttttga
     1381 tttggcaggt aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg cggcgcttaa
     1441 cgcagtgata gcagcacacg gtccgggtag cggcaccaca cgaatccccg cttcacggca
     1501 ggtacgcacc agatggtagc caggatcgtt aattagcggc gttccggcat cggaaaccag
     1561 cgcaatgttt tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt
     1621 atggtcgtgc agcgcaaaca accgggcatt aatcccaaaa tgttgcagca ataaaccggt
     1681 gtgacgagta tcctcggcgg caatcagatc aacggcctgt aatacctcta acgcacgctg
     1741 ggtgatatcc gccagattgc cgattggcgt cggtacaatg taaagctggc cctgagaatt
     1801 atccgccgat tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat attgagcatt
     1861 gcgtaaaaaa atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc
     1921 gcgcgttgtc tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact
     1981 cccgatcagt ccactgctta tatgcagggc acggcgcagg ctgattctgc cttttatctt
     2041 cagcagatgc agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt
     2101 gcactggtga aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa
     2161 gaactgaacg attctcagcg tcgcgagaaa acactgctgg cggtagagat taaactggcg
     2221 cagaaagatt ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa
     2281 caaaaccagc aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc
     2341 attgatttac tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa agaaaaaaag
     2401 cagaatattg atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg
     2461 ctggtgatca acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg
     2521 tttgataacc gtaacgatcc cgacatgatg aaagccggga tcgccgactg gcaaaaacgt
     2581 tatccgaaca atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt
     2641 aaaccagcct cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta
     2701 tttggtcgca ccattcagca aggctttgaa gcggcgaaaa atatcggcac tcagccagtg
     2761 gcggctcagg tagctgccgc acctgccgca gacgtagctg aacaacctca gccgcaaacc
     2821 gtggatggcg ttgccagccc ggcacaagcc tcggttagcg atctgaccgg tgatcagcct
     2881 gcggcccagc cggtgcctgt aagcgccccg gcgacaagca ccgcagcggt aagcgcaccc
     2941 gcaaatccat ccgcagagct gaaaatctac gatacctcat cacaaccact tagccagatc
     3001 ttaagccagg ttcagcagga tggcgcgagt attgtggttg gtccgttgct gaaaaataac
     3061 gttgaagagt tgctgaagag caacaccccg ctgaacgtgc tggcactgaa ccagccagag
     3121 aatatcgaaa accgcgtcaa tatttgttac ttcgcgcttt cacctgaaga cgaagcgcgc
     3181 gatgcagcgc gtcatattcg tgaccagggt aaacaagcgc cgctggtgct gatcccacgc
     3241 agttcattgg gcgatcgcgt agccaatgcg tttgcgcaag agtggcagaa actgggcggc
     3301 ggcaccgttc tgcaacaaaa atttggttcc accagcgaat tacgcgcggg tgttaacggc
     3361 ggttctggta tcgctttaac gggtaccccg attactccca gagcgacaac cgactccggc
     3421 atgacgacca acaatccaac gctgcaaacc acgccaaccg atgaccagtt caccaataat
     3481 ggcggtcgtg tcgatgcggt gtacattgtg gcaacgccgg gtgaaatcgc ttttatcaaa
     3541 ccgatgatcg ccatgcgtaa cggtagccag agcggtgcaa tgctgtacgc cagctcccgc
     3601 agcgcacaag gcaccgcagg cccggatttc cgtctggaga tggaaggttt gcagtacagc
     3661 gaaatcccga tgctggcggg cggtaatctg ccgttaatgc agcaggcact cagcgcggtg
     3721 aataacgatt attcactggc tcgcatgtat gcgatgggcg tcgatgcctg gtcgctggca
     3781 aatcatttct cacaaatgcg ccaggttcag ggttttgaaa tcaacggtaa taccggaagc
     3841 ctgacggcaa acccggattg cgtgattaac aggaagttat catggctaca gtaccaacaa
     3901 ggtcaggtag tccccgtcag ttaaccacca aacagaccgg cgatgcgtgg gaagcacaag
     3961 cgcgtcgctg gctggaaggc aaaggactgc ggtttatcgc cgctaacgtg aacgagcgtg
     4021 gcggcgagat cgatctgata atgcgtgaag gccggaccac cgtttttgtc gaggtacgct
     4081 atcgccgctc tgcgctttat ggcggcgcgg cagccagtgt gacccgcagc aaacaacaca
     4141 aattattaca gactgcccgc ttgtggctcg cgcgtcataa tgggagtttt gatactgtgg
     4201 attgccggtt cgatgtggta gccttcaccg ggaatgaggt tgagtggatt aaggatgcct
     4261 ttaatgacca ctcataatta aggtttaagg attagcgtgc aagaaagaat taaagcttgc
     4321 ttcactgaaa gcattcaaac tcaaattgcg gcggcagagg cgcttccgga tgccatctcc
     4381 cgtgcagcca tgacgctggt tcagtctctg ctcaatggca acaaaatcct ctgttgtggt
     4441 aatggaactt ccgctgccaa tgcacagcat tttgctgcca gcatgatcaa ccgtttcgaa
     4501 acggagcggc ccagcttacc tgccattgca ctaaatactg ataatgttgt cttaacggcg
     4561 attgccaacg atcgcttaca tgatgaagtg tatgcaaaac aggtgcgggc gctgggtcat
     4621 gcgggagatg tattgttagc catttccacc cgtggcaaca gccgcgatat tgttaaagca
     4681 gttgaagccg ccgttacgcg tgatatgacc attgtggcat tgaccggcta tgacggcggc
     4741 gagcttgcag gtttgttagg gccacaggat gtggagatcc gcattccttc gcatcgtagt
     4801 gctcgcattc aggaaatgca tatgctgacg gtaaattgcc tgtgcgatct gatcgataac
     4861 acgcttttcc ctcaccagga tgattaagga gaatacatga aggcattatc gccaatcgca
     4921 gtccttattt ccgcgctgct gttgcaaggt tgtgttgccg ctgccgtagt gggtaccgct
     4981 gctgtgggta ccaaagccgc aactgaccca cgcagtgtcg gcacccaggt ggacgatggt
     5041 accctggaag tgcgcgtgaa cagcgcattg tcgaaagacg aacagattaa gaaagaaacc
     5101 cgtattaatg tcacggcgta tcagggcaaa gtgctgctgg ttgggcagtc accaaacgct
     5161 gaactttcgg ctcgcgccaa acagattgct atgggcgtag acggtgccaa cgaagtgtat
     5221 aacgagattc gtcagggcca gccgattggt ctgggcgaag catctaacga tacgtggatc
     5281 accaccaaag tgcgttcgca gctcttaacc agcgacctgg tgaaatcgtc caacgtgaaa
     5341 gtgaccaccg aaaacggtga agtgttcctg atggggctgg tgactgaacg tgaagcgaaa
     5401 gcggcggcag atattgccag ccgggtgagc ggcgtaaagc gggtaaccac agcgtttacg
     5461 tttattaaat agcagcccct tgtaatgcct gatgcgacgc ttgccgcgtc ttatcaggcc
     5521 tacaacgatg caaaccgtag gtcggataag gcgtttacgc cgcatccgac atgttttccc
     5581 tcaaatcaga ataacagcgc cagcccgccg acaatcacgc ctgacactgc caccatcgcc
     5641 cccgtcagcc ataaggcttt cgccgggaac gctttgcgca gcataatcag tgacggcaaa
     5701 ctcactgccg ggagcgtcat caacaatgcc agcgccggag cggttcccat acctgccagc
     5761 atcatcgttt gtacaatcgg aatttctgct gccgtgggaa tgacaaacaa gcatcctgct
     5821 accgccatcg ccaccaccca catcaggctg ttatcgacag caccatcggc atgggggaat
     5881 aaccagacgc gtgcggcacc caacaccagt actgcaagga tgtaaaccgg gatcgtactc
     5941 cagaaaagtg tccatagcgc cctgccccag cggctaaaaa aaccgctctg tgcttccggt
     6001 atgtcaattt cgaccggtgc ctgcgtttgc ggtgtttcac gcacccattt ttgcaccagc
     6061 gtcgcaatca gcaacaccat caccagcccg gccaccagac gaatcgccgc aaaaccccag
     6121 ccgaggacaa accccataaa caccagcgtc gccgggttta acaccggatt gcccatccag
     6181 aatgccagcg caccgcccat cgatacctgt tggcgacgca ttcccgccgc gaccggagcc
     6241 gcacagcagg tacacatcat gcccggcagc gaaaacagcg ttcccagcag cgtgccgcga
     6301 aagcgcgatt gtccaagcgt acgcaacaac cagtcacgcg ggatcagcac ctgaatcaac
     6361 gaaccgagga tcacccccag caccgccgct ttccataccg cgaggaaata gatcatcgcg
     6421 taatccaacg ccgcctgcca cggattagca tccgcctgcg caaggataga tttaccgata
     6481 ctgtgggttt cggcagcagt aaacgctttg ccgtagtaag gttgccattt cacataccag
     6541 agaccggcaa tgacaacgag aaagaaaagc gcgggtttcc accactgaat gggcgttgcc
     6601 gcctgagatg aagactgacc agtcatagca ttccccagga ataagttatg ataattgagc
     6661 gcgtgaatat tacgctcact atcaattctt gggaataatt attcagctct tttgcgtaat
     6721 tctgaagagc ttaaaatagt cacgccttca tgctcaggcc gcatcgcctc tgccagcatg
     6781 acccgcgcca cgtctctcgc atcaatggat ttccagttac cgggcagtaa acgaaacagc
     6841 ggcgcaaaaa gcgtttcgtt catccgctgt ttgctacgat cgcccagcaa catcgacggg
     6901 cgagcaatgg tcaatttcgg ccagttctgg gcaattaatg cttcttccat ctcccctttg
     6961 acgcggttat agaaaaacgg cgagtgggca ttggcaccca tagcactgac caccagcata
     7021 tgctgcgcgc ccagtcgccg cccggttaat gcggtatcca ctaccagcgt gtaatcggca
     7081 tgaataaacg cctctttgct ccccgcttct cgccgcgtgg tgccgagaca acaaaacaca
     7141 atgtcgatgg gttcggtgac ctgcgccaac gcgtcactca gttgcggatc atggggatta
     7201 aacaccccgg gcatatcgcc caacggacgt cgcgtcggcg cggctatggc gttaactttc
     7261 ggttcgttaa tcaacatccg cagcaggtga ccgcccacca gccccgttgc gcctgtaatc
     7321 agtacctgac tcatcttcgc tcctttacag aattgtctgc cttgcgctcc acggctcatg
     7381 caccaggctt aatagaccat gaggtaatta tccccgattg tgtgaaattc gcctcatcca
     7441 atgcaacaac gcggaggaag catgagtaag aaaattgccg ttttaatcag tgatgaattt
     7501 gaggattcag aatttacttc acccgcagac gagttccgta aagccggaca cgaagtgatt
     7561 accattgaaa aacaagcggg taaaacggtg aaaggcaaaa aaggagaagc cagcgtgacc
     7621 atcgataaat ccatcgatga agtgacgcca gcggagtttg atgccctgct gctaccgggt
     7681 ggtcattcac cggattatct gcgtggcgac aaccgttttg tcacctttac ccgtgatttt
     7741 gtgaatagcg gcaaacctgt tttcgccatc tgtcacggtc cgcagctgct gatcagcgcc
     7801 gatgtgattc gggggcgcaa actgaccgca gttaaaccga tcattattga tgttaaaaat
     7861 gcgggcgcgg aattttacga tcaggaagtc gtggttgata aagatcagct ggttaccagc
     7921 cggacaccgg acgatctgcc agcgtttaac cgcgaggcgt tacgcctgct cggtgcctga
     7981 gtcgcgtagc caaatcattt ttttaccaaa gcccagcgtg ttgtcagtga atttgatttc
     8041 atcgagccgg atttcccaca ccggggccga cagcattctg gcaaccggaa agcgacgatt
     8101 gtacgcctgg cgcgcgaggt cgctttcctc accttccagc ctgcggatct cacctttaaa
     8161 ctgcacgccg cgaattaacg ctaccgtttt cggctgaccg tttaccgttc cggcaaccgc
     8221 agcctgcggc ccactcatct gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc
     8281 gactttctgc gcatcaaaca gataaaaagc attagcgcac cacagttccc cttcctgctg
     8341 cacacaccag gtgacaacat gttgttttgc caaccaacgg ctgatggtga tgagtgtttc
     8401 cattgctgct ctcttttata ctgtgggcct gaccttaaca tgcgacgcag acgatgacac
     8461 cctggtttct ttacttgatc cgtaccgccg acaataagct ttataccggg atcaccacgg
     8521 atgtcgaacg ccgctatcag cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga
     8581 aaggagaact gacgctggcg ttttctgcgc cagtgggcga tcgttcgctg gcgttacggg
     8641 cggaatatcg cgttaagcaa ctgaccaaac ggcaaaaaga gcgtctggta gcggaaggcg
     8701 cagggtttgc agagctgtta agcagtctgc aaaccccgga gattaaaagc gattgaaatg
     8761 ctcgtgatac tcaaccagac cggttacgcc attcagcgcg tcatccgcta aacgatgtac
     8821 ctggaaggcg ctttcagtgc ccggccagcg gcaacgcaga tcgtgatgcg ccgccagttc
     8881 aaagccgaaa cgactgtaca gcgccggatc gcccagcgtc accactgcgg catagccgaa
     8941 ctcattaagc gaatcgagtc cttcatagac cagttggcgt gccagcccct gtccacggta
     9001 tttttcatca accgccaacg gtgccatgcc gacccattgc aggtcttcgc cctgcacatc
     9061 aaccggacta aatgccacat agccaatgac ctgaccttcg tcatctgtcg ccaccagccc
     9121 cagcgtcaga aagccatctt cacgcagatc gtgaaccagc ttcgcttccg catcactttc
     9181 gaatgagcga cgcagcaggg catcaatacc cggcgcatca atgggaattt ctactcgaat
     9241 tagcatggtt cacctaccga tgtctgtttg gtttcaggcg cggttttcat tcccgcctca
     9301 acaaaatccg ccagttgcag cagcatcatg cgtaaggctt tcggcatttg ctccagttca
     9361 atggcgtcca tcaggttttt cacatacagc cccagctccg tatcgccttc aatcaccagc
     9421 cgacgctgga agaagagcgt atccggatct tgtttacgcg ccgcaatcat cagcagatcg
     9481 ctggcgtcgg cactaaaact cacatcagct tgcgcgttct ggctgaccac cagtttgcca
     9541 ttcaccaccg aggtaaacca ttgcaggtca atatcacgca catgaatact taaccagcgg
     9601 ccttcaagaa actccagctc accatcatcc agcgcctgac ggaattgcca gcttaagacc
     9661 tgctcaagaa cctggcgttt tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga
     9721 gatggcccca aatgcacaat acgggaacgc agtttatcca acacgagttt tactccctgt
     9781 ttcaacaatc atcctatttt gccatatcag aaaaataaca tagcggtata aatcaacaat
     9841 tccatatgaa attgctgcta ccaccaatac aactttaact gccttaaatc aaaaattgtc
     9901 gcagcaaggt taactaaaat cccagttcgt taacattttt gcgttttgaa agcgcaacct
     9961 tcaggaaaaa ttatggagct gctctgccct gccggaaatc tcccggcgct taaggcggcc
    10021 atcgaaaacg gcgcagatgc tgtttatatc gggctaaaag atgatactaa tgcccgtcac
    10081 ttcgccggcc ttaactttac cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa
    10141 catcgccgca aacttcacat cgcgattaac acttttgcgc atccggacgg ttacgcccgt
    10201 tggcagcgcg ccgtggatat ggcggcgcag ctgggtgccg acgcgctgat cctcgccgac
    10261 ctcgccatgc tggagtacgc cgccgagcgt tacccgcata ttgagcgtca tgtgtcagtg
    10321 caggcttcgg cgaccaatga agaggcgatt aacttttatc atcgccattt tgatgttgcc
    10381 cgcgtggtgc tgccgcgcgt gttgtcgatt catcaggtga aacagctggc acgggtcaca
    10441 cctgtcccgc tggaagtctt tgcttttggc agcctgtgca ttatgtcgga aggtcgttgc
    10501 tatctgtcgt cgtatctgac gggtgagtcg cccaacaccg tgggcgcgtg ttctccggcc
    10561 cgtttcgtgc gctggcaaca aacgccgcag gggctggaat cccgcctgaa cgaagtgctg
    10621 atcgaccgtt atcaggatgg cgaaaacgca ggttatccga cgctgtgtaa agggcgttat
    10681 ctggtggacg gcgagcgcta tcacgcgctg gaagaaccaa ccagtctcaa taccctggaa
    10741 ctgctgccgg agttaatggc ggcgaatatt gcttcggtga aaattgaagg ccgccagcgt
    10801 agcccggcgt atgtcagcca ggtggcgaaa gtctggcgtc aggctatcga ccgttgtaag
    10861 gccgatccgc aaaacttcgt accgcaaagc gcgtggatgg agacgctcgg gtcgatgtcc
    10921 gaaggcacgc agaccaccct tggcgcgtat caccgtaaat ggcagtgaga agagcaatga
    10981 aatattcctt agggccagtg ctgtggtact ggccaaaaga ggcgctggaa gaattttatc
    11041 agcaggccgc caccagcagc gccgacgtga tttatcttgg tgaagcggta tgcagcaagc
    11101 gccgggcaac caaagttggc gactggctgg agatggcaaa atcgctcgcc ggaagcggga
    11161 agcagatagt gctctccacg ctggcgctgg tgcaggcatc ctctgaactg ggcgaactga
    11221 aacgctatgt tgagaacggt gagtttctga ttgaagccag cgatctcggc gtggtgaata
    11281 tgtgcgctga acgcaaactg ccgttcgtcg ccgggcacgc gctgaactgc tacaacgcgg
    11341 tgacgctgaa aatattgctc aaacagggca tgatgcgctg gtgtatgccg gtggagcttt
    11401 cccgcgactg gctggtgaat ctgcttaatc agtgcgataa gttggggatt cgcaaccagt
    11461 ttgaagtgga agtcctgagc tacggtcatc taccgctggc ctactccgcc cgctgcttta
    11521 ccgcgcgttc ggaagaccgc ccgaaagatg agtgtgaaac ctgctgcatt aagtatccga
    11581 acgggcgcaa cgtgctgtcg caggaaaacc aacaagtgtt tgtactcaat ggcattcaga
    11641 ccatgagcgg ctacgtttac aacctcggta acgagctgac atccatgcag ggattggttg
    11701 atgtggtccg cctgtcaccg cagggtactg acactttcgc gatgctcgac gccttccgcg
    11761 ctaatgaaaa tggcgctgcg ccactgccgt tgacggcaaa cagcgactgt aacggctact
    11821 ggcggcggct ggctgggctg gagctgcaag cctaagtaaa tatgctcgtc atacttcaag
    11881 ttgcatgtgc tgcatctgca ttcgttcacc ccagtcactt acttatgtaa gctcctgggg
    11941 attcactctc ttgtcgcctt cctgcaactc gaattattta gagcacagct cactttgtta
    12001 acaactttaa ctactcttta atgcagtatt aaagattaat cggtaacaaa gtgagctgtt
    12061 atgactgata aaaccattgc gttttcgcta ctcgatctgg cccccattcc cgaaggttct
    12121 tcagcgcgag aagcattctc ccactctctc gatctcgccc gtctggctga aaagcgcggc
    12181 tatcatcgct actggctggc agaacaccac aatatgactg gcattgccag tgctgccacg
    12241 tcggtattga ttggctatct ggcggcgaat accaccacgc tgcatctggg gtctggcggc
    12301 gtgatgttgc ctaaccactc accgttggtc attgccgaac agttcggcac gctcaataca
    12361 ctctatccgg ggcgaatcga tttggggctg ggtcgtgcgc caggtagtga ccaacggaca
    12421 atgatggcgc tgcgtcgcca tatgagcggc gatattgata atttcccccg cgatgtcgcg
    12481 gagctggtgg actggtttga cgcccgcgat cccaatccgc atgtgcgccc ggtaccaggc
    12541 tatggcgaga aaatccccgt gtggttgtta ggctccagcc tttacagcgc gcaactggcg
    12601 gcgcagcttg gtctgccgtt cgcgtttgcc tcacacttcg cgccggatat gttgttccag
    12661 gcgctgcatc tttatcgcag caacttcaaa ccgtcggcac gactggaaaa accatacgcg
    12721 atggtgtgca tcaatattat cgccgccgac agcaaccgcg acgctgaatt tctatttacc
    12781 tcaatgcagc aagcctttgt gaagctgcgc cgtggcgaaa ccggacaact gccgccgccg
    12841 attcaaaata tggatcagtt ctggtcaccg tctgagcagt atggcgtgca gcaggcgctg
    12901 agtatgtcgt tggtgggtga taaagcgaaa gtgcgtcacg gcttgcagtc gatcctgcgc
    12961 gaaaccgacg ccgatgaaat tatggttaac gggcagattt tcgaccacca ggcgcgcctg
    13021 cattcgtttg aactggcgat ggatgtgaag gaagagttgt tgggatagtg tgtcttaacg
    13081 cgggaagcct tatccgagct ggcaacgctg tcctacgtag acctgataag cgaagcgcat
    13141 caggcattgt gtaggcagca gaaatgtcgg ataaggcacc gctgattact gatacaccgg
    13201 cagcaaatta aagctcgata agatatggac cagtgcgttg ccaacgccaa acaccagaat
    13261 cagcgtaatc atcggcttgc cgccccagac acggaatttc gggctgccaa agcgtttacg
    13321 cgatgcgcgg gctaacagcg ccggaacaat tgccgcccag atggtagctg ctaaaccagc
    13381 ataaccaatg gcgtacagga atccgttcgg gaacagtaga ccacccacaa caggcggggc
    13441 aaaggtcagc aatgccgttt tcaagcgccc cagggctgag tcatcgaaac caaacagatc
    13501 tgccagatag tcaaacaaac ccagcgttac gccaaggaac gaactcgcta ccgcaaagtt
    13561 tgagaacacg accagcagaa ggtccaggct acggctgttc agcacgccgc ttaatgcctg
    13621 taccagcaca tcaatattac cgcccttctc tgcaataccg ataaactccg gacgcgggat
    13681 gttacccatc gtcgccagca accagatggt atacagcgcc agtgccatca gcgtaccgta
    13741 caccaggcat ttcacgatgg ttttcggatc tttgccgtaa tacttcatca ggctcggcac
    13801 gttaccgtga taaccaaacg atgccagaca gaacggcagg gtcatcagca aatacggcgc
    13861 ataagatgca ttgctttcgg cgacgttgaa caatgtcgca ggctgcacat gccccagtag
    13921 gctaccgaag gtgaggaaga aggtaatgac tttcgccccc agtacaatcg ccgtcatgcg
    13981 gctgacggcc ttagtactca accacaccac aaacgctacc agcagtgcaa aaccaaaacc
    14041 cgccgcccgt gccgggacgt ttagcgacat ctctgcgaag gtgtgatgca gaattgaacc
    14101 actggcagaa atataggcat aggtcaggat atagagcaca aaggcaatgg aaataccgtt
    14161 gaccacgttc cagcctttgc ccagcaaatc tttggtgatg gtgtcaaaac tcgaaccgat
    14221 tcggtaattc aggttagctt ccagaatcat caagccggaa tgcagcatac agaaccaggt
    14281 aaagatcagc gccgccattg accagaaaaa ccacgccccg gacatgacca ctggcagaga
    14341 aaacatccct gcgccaataa tggtgccgcc gataatcacc acgccgccaa gcagcgacgg
    14401 tgacgtttgg gtggtggtta gtgttgccat gagagcttct ctccagtgaa aaatagtgcg
    14461 actgcgttgt tatgcattgc actgtaccag tacacgagta caaaagacag aaaaaaagcc
    14521 ccgatggtaa aaatcggggc tgtatatatt attttacaga ttgtgttcgc tgttcagcga
    14581 tgattacgca tcaccaccga aacgacgacg accggtagag tcatcacgac gcggagcgcg
    14641 gccttcacga cgttcgccgc taaaacgacg accatcacca cggccacctt cacggcgttc
    14701 accgctgaag ttacgaccgc cttcacgacg ttcgccaccg aaaccacgac caccgccacg
    14761 acgctcaccg ccagtatgcg ggtgtgcatc gcccagtaac tgcatgttca tcggcttgtt
    14821 gagaatgcga gtgcgcgtaa agtgttgcag cacttcaccc ggcatacctt tcggcagttc
    14881 gatggtggag tgagaagcaa acagcttgat gttaccaatg taacggctac tgatgtcgcc
    14941 ttcgttagca atcgcaccaa cgatatgacg aacttcaaca ccatcatcgc ggcccacttc
    15001 aatgcggtac agctgcatat cgccaacatc acgacgttca cgacgcggac gatcttcacg
    15061 gtcaccacgc gggccacggt cgttacgatc gcgcggacca cggtcatcac ggtcacggaa
    15121 ttcacgtttc ggacgcatcg gcgcatctgg cggtacgatc agagtacgtt caccctgtgc
    15181 cattttcagc agtgccgcag ccagagtttc gagatccagc tcttcacctt ctgcagtcgg
    15241 ctgaattttg ctcagcagtg cgcggtattg atccagatcg ctgctttcca gctgctgctg
    15301 tactttagcg gcgaattttt ccagacggcg tttgcccagc agttctgcgt tcggcagttc
    15361 tacttccgga atagtcagct tcatagtacg ttcaatgttg cgcagcagac gacgctcgcg
    15421 gttctcaacg aacagcagcg cgcggccagc acgacccgca cgaccggtac gaccgatacg
    15481 gtgaacgtaa gactcagaat ccatcgggat atcgtagtta actaccaggc tgatacgctc
    15541 aacgtccagg ccacgggctg caacgtcggt cgcaatcagg atgtccagac gaccatcttt
    15601 caggcgttcc agtgtctgtt cacgcagcgc ctggttcatg tcaccgttca gcgcggcgct
    15661 gttgtagccg ttacgctcaa gagcttcagc cacttccaga gtcgcgtttt tggtacgaac
    15721 gaagataatc gccgcatcaa aatcttccgc ttccaggaaa cgtaccagtg cttcgttttt
    15781 gcgcataccc cagacagtcc agtagctctg gctgatgtca ggacgggtag tcacgctgga
    15841 ctgaatgcgc acttcctgcg gctctttcat aaagcggcgg gtaatgcgac gaatcgcttc
    15901 cggcatggtt gcagagaaca gagcggtctg atgaccttcc gggatctgcg ccataatggt
    15961 ttcaacgtct tcgatgaagc ccatgcgcag catttcgtca gcttcatcca gaaccagacc
    16021 gctcagttta gagaggtcca gagtgccacg tttcaggtgg tccagcagac ggcccggagt
    16081 accgacaacg atctgcggcc cctgacgcag ggcgcgtaat tgcacgtcat aacgctggcc
    16141 gccgtacaga gcaaccacat ttacgccgcg catgtgttta gagaaatccg tcattgcttc
    16201 agcaacctgt accgccagtt cgcgggtcgg tgccagcacc agaatctgtg gtgctttcag
    16261 ctcaggatca agattctgca acagaggtaa agagaatgct gcagtttttc cgctccccgt
    16321 ctgggccata cccagaacgt cgcggccatt cagcagatgt ggaatacact ctgcctgaat
    16381 tggagatggt ttttcgtaac ccagatcgtt aagggcttca aggataggag ccttcaggcc
    16441 cagatctgca aaagtggttt cgaattcagc catgtagtac gtgtgcctca aaattaatgg
    16501 cggccagtct acataactca tcatgaaatt gatcagcaat tttcattgaa aagtgtgaac
    16561 cggctcaaag taggtgtatt aacgaacaac aacgccctca cccgttaagg tgatggcaat
    16621 caaaaaagat tacgggctga tgtgtacgtc agctattgct ggtccgattc tgccaggtca
    16681 tcttggtcct ggcccaggag cgataattcc aacaatgcgt atcggtgctc aacaaagtta
    16741 tgaacgttgt tggcaaccgc cagtttgaac agtgccgtgg cgctgtccaa atcccccaga
    16801 cttaggtagt acttacctaa atagaagttg gtttcactga gatgctcagc gagcgaggtg
    16861 ttatccgttg cgtccgcctt gagcctttcc attaacgttt gttcgctaat gttgcccagg
    16921 tagaactcga caatgttcca tccccactgt tccttatccg atttttcgaa gtgctgtttc
    16981 aacacttctt tagcctgctt ctcatcgagc ttctgctcgg cgagataaag ccacagacta
    17041 cggaaaggat cattgggatc gtcttgataa aacgccagca gatcatcttg cgctaacttg
    17101 tcacgaccgc cgtaatataa tgcgatcccg cgattcaagt gcgcgtagtt gtaagttgga
    17161 tcaagctcaa gtacagaatc aaacgcttca taggcagcat caaaattgcc tgcctgcgtt
    17221 aaatatatgc ctaagtaatt gaatacttca ggcatatccg gtcggattgc cagcgcttgc
    17281 gaaaaatcgt tacgcgctaa tgccctcaga ccgagactat catacaacac tccgcgctca
    17341 tataaaagct gtgcgcgttc gtcatcggtt aaagcccgac tggcaaggat ttgttccata
    17401 cgtgccagaa tcacttcctg ctgtaaagtc ggttgcaatg gtaccgcgag gacttcactt
    17461 ttacgccagg aagtattact gcatcctgca agcgtaagtg ctgtcgcaac gaaacaccag
    17521 cgcaaaaaag gcttcatttc ccactcccga agaccacggt tgaatgaacg tcctgttccc
    17581 ggttgctaac aaggcgtcct gcccggttaa aagccccccg ccgcagcgga gggcaaatgg
    17641 caaccttact cgccctgttc agcagccgga gcttccggtg ctgcagcagg ttgagactgc
    17701 tcagtcgctt ctttaatgct cagacggata cggccctggc gatcaacttc tagaactttc
    17761 accggtactt cctgacccat ctgcaggtaa tcggtcactt tctcaacgcg tttgtcagcg
    17821 atttgagaga tgtggaccag accttcttta ccgccgccga tggcaacaaa tgcgccaaag
    17881 tcaacgatac gggtcacttt accagtgtag acgcggccca cttcgatttc tgcagtgatc
    17941 tcttcgatac gacgaatagc atgtttcgct ttctcgccgt cggtcgctgc gatcttcaca
    18001 gtaccgtcat cttcgatttc gatggtagtg ccagtttctt cggtcagggc acggataaca
    18061 gagccgcctt taccgataac gtctttgatc ttatccgggt tgatcttgat ggtatggata
    18121 cgcggtgcga actcagagat atcgccacgc ggcgcgttga tcgcctgttc cattacgccc
    18181 aggatatgca gacgcgcacc tttagcctgg ttcagcgcaa cctgcatgat ctctttggtg
    18241 ataccttcaa ttttgatatc catctgcagt gcagagatac cgtcgcggga acctgcaact
    18301 ttgaagtcca tatcgcccag gtgatcttcg tcgcccaaaa tgtcagacag tacaacgtag
    18361 ttgtcgcctt ctttcaccag acccattgcg atacccgcaa cggcagcttt gattggcaca
    18421 cctgcgtcca tcagcgccag agacgcgccg cacacggaag ccatagaaga agaaccgttg
    18481 gattcggtga tttcagacac tacacgtacg gtgtacggga atttgtccat atccggcatg
    18541 actgccagca cgccgcgctt cgccagacga ccgtgaccaa tttcacgacg cttcggagaa
    18601 ccgaccatgc cggtttcgcc tacggagtac ggagggaagt tgtagtggaa caggaaggta
    18661 tcggtacgtt cgcccatcag ttcatcaaga acctgcgcgt cacgagcggt acccagcgtt
    18721 gcagtaacca gtgcctgcgt ttcaccgcgg gtgaacagcg cagaaccgtg agtacgcggc
    18781 agcacgccag tacgcacatc cagaccacgg atcatatctt tttcacgacc gtcgatacgc
    18841 ggttcgcctg ccagtacgcg gctacgaaca acgtttttct cgatagcgtg cagaatttca
    18901 cccagttcgt tttcgtccag ggtttcgtct tcagcaagca gcgtcgcgat ggtttcagat
    18961 ttgatgacat caacctgcgc ataacgctct tgtttgtcgg tgatgcggta agcatcactc
    19021 agacgagctt cagccagtgc agcaacgcgc gcgttcagcg cttcgtttac cggctccggc
    19081 tgccagtccc aacgcggttt accggcttct ttcactagtt cattgatgtt ctgaataaca
    19141 acctgctgtt gttcatgacc gaacactact gcgcccagca tctgatcttc gctcagcagt
    19201 tcagcttcag attcaaccat cagtacagcg gcttcagtac cggcaacaac cagatccagt
    19261 ttgctctctt tcagctcgtc ctgagtcggg ttcagtacgt actggtcatt gatgtaacct
    19321 acgcgggcag caccaatcgg gccattgaac ggaataccag acagagacag tgctgcggaa
    19381 gcaccaatca tcgcgacgat atccgggtta acctgcgggt taacagaaac cacggtggcg
    19441 ataacctgaa cttcgttgac gaagccttcc gggaacagcg ggcgaatcgg gcggtcaatc
    19501 agacgcgcga tcagggtttc gccttcgctt ggacgacctt cacgacggaa gaagctaccc
    19561 gggatacgac cagcagcgta ggtacgctcc tgatagttaa cggtcagtgg gaagaagtcc
    19621 tgacctggtt tggctttttt ctgtccaaca acggtaacga ataccgcggt gtcatccatg
    19681 ctaaccataa cagcggcagt agcctgacga gccatcatgc cggtttccag agtcacggtg
    19741 tgttggccgt actggaattt acgaacgatc ggattaagca atgtaatatc ctttctcttt
    19801 cttagacagt accttacggc actggtgtta atacccgatc ttctgcgcat cctcgcgact
    19861 aatgacaacc ctaacccagc tctatgtggg taaagcctct cattagccgc gcgaacctct
    19921 gcaacggaag atcattcata gcaacaatac attagtttcc agtgaattgc tgccgtcagc
    19981 ttgaaaaaag gggccactca ggcccccttt tctgaaactc gcaagaatta gcgacgcaga
    20041 cccaggcgct cgatgagctg ggtgtaacgt gctacgtctt tacgtttcag gtagtcgagc
    20101 agtttacgac gctgagaaac catgcgcagc agaccacgac ggctgtggtg atcttttttg
    20161 tgctctgcaa agtggccctg caggtggttg atctgtgcag tcagcagtgc tacctgaact
    20221 tcggtagaac cggtgtcgtt tgcgtcacga ccaaactcag aaacgatttt agctgttgct
    20281 tcagtactta gagacatttt aaaactccaa agtatataga atgaaaggac gccgatctct
    20341 aattcagcga tcccagtgta cgttacgcaa agtgttaaac aatttacgcg acgttaagcg
    20401 gcagtattct actcgtagcg acctgttatc gcaagacggt taacattacg ccgggtattc
    20461 aaccaccagg cgacgaggcg cgacgcggcc ttcatcgtca atttcgccca taccgataaa
    20521 cttgccgttc tcaccttctg taacgcgaac cagtccttcc agcggcgcac cggatgtacg
    20581 aactgggtta ccatttttga agtaaacaga agacgttaac ggaagattca ccaccgggta
    20641 gtccgaagct ggactgtcca ttggcatcag taatggatca agtaactccg cagccggaat
    20701 atcctgctgt tcggcttgtt cgaccagttc acgcagatgt tccagggtca ccatccgttc
    20761 aaccggatat ttacttaccg ccagacgacg caggtaaata acatgcgcgc cacaaccgag
    20821 tttttcaccc aggtcatcaa tgatggtgcg gatataagtg ccttttgagc agtgaatttc
    20881 cagctccagc tcattgcctt cgtggcgaat aaacagcaat tcataaacgg taatcgggcg
    20941 cgcttcacgc ggtacttcaa tgccctgacg cgcatattcg tacagttttt taccctgata
    21001 tttcagtgct gaatacatcg aagggatctg ttcgatatcg ccacggaaag tatccagcgc
    21061 cgccgccagt tgctctgcgc taaaggttac cggacgttct tcaacgattt gtccgtcggc
    21121 atcagaagta tcggtacgct gtccaagacg cgcaatgacc cgatagcgtt tgtcggagtc
    21181 cagcagatac tgggaaaact tcgtcgcttc cccgaggcaa attggcaaca tgcctgttgc
    21241 cagcgggtcc agcgcaccgg tatgcccggc acggttggcg ttataaatcc gtttcacttt
    21301 ttgcagcgca tcattgctgg acataccctg cggtttatcc agcaacaaaa cgccgttaat
    21361 gtcgcgaccg cgacgacgag gacgactcat tagtcctcct tgctgtcgtc cgggttaaca
    21421 cgacgttctt cgtcatgttt gaccacgctg gtcaccaggt ttgacatgcg catcccttca
    21481 accagagagt tgtcgtagaa gaaggtcagt tccggcacga tacgcaggcg catcgctttc
    21541 cccagcaggc tgcggatgaa accagaagct tcttgcaacg ctttgatgcc cgctttaacc
    21601 gcgtcttcat ctttgtcgtt gaggaacgtc acatatactt tggcatacgc caggtcgcga
    21661 gacatttcga caccggaaac ggtggtcatc atgcccaggc gaggatcttt aatttcacgc
    21721 tgcaggatga gagcgatctc tttttgcatt tcctgcgcta cgcgctgcgg gcgaccaaat
    21781 tctttcgcca taataaattc tcctgacaaa aaaggggctg ttagcccctt tttaaaatta
    21841 atttcaggtg gaagggctgt tcacgttggc ctgataagac gcgccagcgt cacatcaggc
    21901 aatccatgcc ggatgcggcg taaacgcctt atcccgcatg gaaccctaaa aaccttaagc
    21961 gatggtacgt tggatctcaa tgatttcgaa tacttcgatc acatcgccag tgcggacgtc
    22021 gttgtagttc ttaacgccga taccacattc cataccgtta cggacttcgt taacgtcatc
    22081 tttgaagcgg cgcagggact ccagctcgcc ttcgtagata accacgttgt cacgcagaac
    22141 gcggatcggg ttgtgacgtt taaccacacc ttcggtaacc atacagcctg cgatggcacc
    22201 aaatttcggc gatttgaaca cgtcacgaac ttccgccaga ccgataatct gctgtttcag
    22261 ttccggagac agcataccgc tcatcgccgc tttcacttcg tcaatcaggt tatagatgac
    22321 ggagtagtaa cgcagatcca agctttccgc ttcaatcact ttacgtgcag aggcatcagc
    22381 acgtacgttg aagccaacca ggatggcgtt ggacgccgca gccagggtgg cgtcggtttc
    22441 ggtgatacca cctacgccag aaccgatgat cttcacttta acttcgtcag tagacagttt
    22501 cagcaaggag tcggagatcg cttccacaga accctgtacg tctgccttca ggacgatgtt
    22561 cacttcgtga acttcgcctt cggtcatgtt ggcgaacatg ttctcgagtt tagatttctg
    22621 ctgacgcgcc agtttaactt cgcggaattt accctgacga tacagtgcaa cttcacgcgc
    22681 tttcttctcg tcacgtacaa cggtaacttc atcacccgca gccggtacgc cggacaggcc
    22741 gaggatttcc accggaatgg acggacccgc ttccagcact tcctgaccca gttcgttacg
    22801 catcgcacga acacgaccgt attcgaagcc acacagaacg atatcgccct tgtgcagagt
    22861 accttcacgt accagaacgg tagcaaccgg accacgacct ttatcgagga aggattcgat
    22921 aaccgcaccg ctcgccatac ctttacgtac cgctttcagc tccagaactt ccgcctgcag
    22981 caggatagcg tccagcagtt catcgatgcc ggtacccgct ttcgcagata cgtgtacgaa
    23041 ctggctttca ccgccccact cttccggcag gatgccatac tgggagagtt cgttcttaac
    23101 acgatccgga tcggcttctg gtttatcaat cttgttcact gcaaccacca ccggtacctg
    23161 cgccgctttc gcgtgctgga ttgcttcgat ggtctgcggc atcacgccgt cgtcggcagc
    23221 aacaaccagg actacgatgt ccgttgcctg cgcaccacgt gcacgcattg aagtaaacgc
    23281 ggcgtgcccc ggggtgtcca ggaaggtgat catgccgttt tcagtttcaa cgtggtatgc
    23341 accaatgtgc tgggtaatgc cgcccgcttc gccagaggca actttcgttg aacgaatgta
    23401 gtccagcaga gaggttttac cgtggtcaac gtgacccatg atggtcacaa ccggcgcgcg
    23461 cggttcagcc gcagcacccg tgtcacggtc gctcattacc gcctcttcca gctcgttttc
    23521 acgacgcagg ataactttgt ggcccatctc ttcagcaacc agctgtgcgg tttcctgatc
    23581 gataacctgg ttgatggttg ccattgcgcc cagtttcatc atcgctttga tgacctgaga
    23641 gcctttaacc gccatcttgt tcgccagttc gccaacggtg atggtttcgc cgatcacaac
    23701 gtcacggtta acggcctgag ctggcttctg gaagccttgc tgcagggaag aacctttacg
    23761 ttttccgcct ttaccgccac gtactgctgc gcgtgcttct tcacgatcag cttttgattc
    23821 agcgtgtttg ttgcctttct tcggacgcgc tgctttcgcg ttacgaccac ggccacgacc
    23881 gccttcgact tcacgatcgc tttcgtcttc tgcctggcga gcatgttgag aagtagtgac
    23941 gtgataatcg ctggaatctt cagtcggttc cgcgttatca gtccatttgt tttcttccgc
    24001 catacgacgt gcttcttcag caacgcgacg tgcttcttct tcgagtttac gatgcgcttc
    24061 ttcttcggct ttacgcttga gctctgcagc ttcctgctca cggcgggctt tttcagcctg
    24121 ggcgttttta gtcatatcgt cttgttgatt gctcactttg tctttttccg cagcttcacg
    24181 tttcgcttgt tcagcagctt cacgcttagc ttgttctgca gcctcacgtt cagctttttg
    24241 ttgcgcctcg cgtttagccg attcttctgc ctcacgacgg gcttgctctt ccgcttcacg
    24301 ctgcgcttgc tcttccgctg caaggcgttc agcctcttgc ggatcgcgtt tcacaaaggt
    24361 gcgtttcttg cggacttcga tttgtaccga tttgcttttt ccaccggtac caggaatgtt
    24421 aagggtgctg cgtgttttac gttgcagcgt caatttgtcc gggcctgaat ttttctgatt
    24481 caggtggtca atcaaagtct gtttctcttg tgcagacaca gagtcgtcag cagacttccg
    24541 gatacctgca tcagcaaatt gctgtaccag gcgttccacg gaggtctgtc gctctgcggc
    24601 cagcgtttta atcgttacat ctgtcatgct gttccttcct gctacagttt attacgcttc
    24661 gtcaccgaac cagcaaatat tacgggcagc cataatcagt gctccggcct tttcgtcggt
    24721 caacccttcg atatcagcca gatcatcaat gccctgttcg gcgagatctt ccagcgtaca
    24781 aacgccacgg gcggccagtt tgaatgccaa atcacgatct accccttcaa ggttcagcag
    24841 atcgtcagcc ggtttgttat caccgaggct ttcttcctgg gcctgtgcaa tggtggccag
    24901 tgcattttta gcacgctcgc gcagtgcttc aacggtcggc tcatcaaggc cttcgatttc
    24961 caacagctct ttcatcggca cataggccag ttcttccagc gtcgagaagc cttcttctac
    25021 cagaacagtc gcgaagtctt cgtcgatgtc gagatatttg gtgaaggtgt cgatcgctgc
    25081 gtgcgcttcc gcctgatgct tagcctgcag gtcgtcaacg gtcatcacgt tgagttccca
    25141 accgctcagc tgcgaagcca gacgcacgtt ctgaccgtta cggccaatcg cctgcgccag
    25201 gttaccggct tcaacggcga tatccatggt gtgtttatct tcatccacca cgatagaagc
    25261 aacgtctgcc ggtgccattg cgttaatcac gaactgcgcc gggttatcat cccacaggac
    25321 gatatcgata cgctcgccac ccagttcagt agacaccgcc tgaacacgcg cgccacgcat
    25381 acctacgcaa gcacctaccg gatcgatacg tttatcgttg gttttcaccg cgattttcgc
    25441 acgagaaccc ggatcgcgag ccgctgcttt aatttcaatc acttcttcgc cgatttctgg
    25501 cacttcaata cggaacagtt cgatcagcat ttccggcttg gaacgagtga cgaacagttg
    25561 cgcgccacgc gcttccgggc gaacggaata aagcacgcca cgaacgcggt cgccagggcg
    25621 gaagttttca cgcggcagca tatcttcacg caggatcacg gcttcagcgt tgttgcccag
    25681 atccagagag atgttgtcgc ggtttacttt tttcaccacg ccggtgatga tttcaccttc
    25741 gtgttcacgg aactgatcaa ccaccatcgc acgttcggct tcacgcactt tctgcacgat
    25801 aacctgtttt gccgtctggg tagtgatacg gtcaaaggta acagactcaa tctgatcttc
    25861 aacgtaatcg cccaggttca ggctttcatc ttcataacgt gcggcttcaa gggtgatttc
    25921 cttggtcggc tgggtgactt catcaacaac taaccagcga cggaaggtgt caaaatcacc
    25981 gcttttgcga tcgatctgta cgcggacgtc gatctcttgt tcatattttt tctttgttgc
    26041 tgtcgccagc gcgctttcca atgcttcgaa aatcttctcg cgaggtagcg ccttttcatt
    26101 ggatacggct tcaactacag ccaaaatttc tttgttcatc gcgggctttt cacctcatcc
    26161 agactattaa aagtggggaa ccaggttcgc cttctggata ttactcagcg cgaacacttc
    26221 atctttacct tcgacggtaa ctgtgatcat ttcaccgtct accgctttga taacgccctg
    26281 ccatttacga cggttttgta ccgccatacg gagaaccaga gtcacctctt ctccgacaaa
    26341 acgggcgtag tgttcagccg tgaacagtgg gcgatcgaga cccggtgagg agacttccag
    26401 gttataagca acggtgatgg gatcttcaac atccagcaca gcacttacct ggtggctcac
    26461 atcagcacaa tcatcaacat tgatgccatc ttcactatca atatagatgc gcagtgtgga
    26521 tgtgcgaccg cgaataaatt cgatgccaac cagttcaaaa cccagggcct caactggcgc
    26581 agtaatcatc tctgttaatt tttgctctaa tgtggacaag cccaccccca agacataaaa
    26641 aaagggccta aagcccagtt attctgtagt cagataacaa aaaaccccga taaatcgggg
    26701 ctttatataa ctgaacccta taaccgcaac tgcggtctgg agcactttcc agaaggattt
    26761 tttcaaatcc cactacgaag gccgaagtct tcacagtata tttgaaaaag gactctaagg
    26821 gaaagtggtt gcgggggccg gatttgaacc gacgaccttc gggttatgag cccgacgagc
    26881 taccaggctg ctccaccccg cgcctgaaac gtggcaaatt ctactcgttt tgggtaaaaa
    26941 atgcaaatac tgctgggatt tggtgtaccg agacgggacg taaaatctgc aggcattata
    27001 gtgatccacg ccacattttg tcaacgttta ttgctaatca tgtgaatgaa tatccagttc
    27061 actttcattt gttgaatact tttgccttct cctgctcccc cttaa
//
LOCUS       DAZPLI010000045        25638 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZPLI010000045 DAZPLI010000000
VERSION     DAZPLI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25638
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.045"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(67..798)
                     /gene="rlmB"
                     /locus_tag="ACHUU3_001807"
     CDS             complement(67..798)
                     /gene="rlmB"
                     /locus_tag="ACHUU3_001807"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR2081893.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(978..3419)
                     /gene="rnr"
                     /locus_tag="ACHUU3_001808"
     CDS             complement(978..3419)
                     /gene="rnr"
                     /locus_tag="ACHUU3_001808"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR2081894.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3458..3883)
                     /gene="nsrR"
                     /locus_tag="ACHUU3_001809"
     CDS             complement(3458..3883)
                     /gene="nsrR"
                     /locus_tag="ACHUU3_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR2081895.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4088..5386)
                     /gene="purA"
                     /locus_tag="ACHUU3_001810"
     CDS             complement(4088..5386)
                     /gene="purA"
                     /locus_tag="ACHUU3_001810"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR2081896.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEIKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5490..5687)
                     /locus_tag="ACHUU3_001811"
     CDS             complement(5490..5687)
                     /locus_tag="ACHUU3_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR2081897.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5769..6773)
                     /gene="hflC"
                     /locus_tag="ACHUU3_001812"
     CDS             complement(5769..6773)
                     /gene="hflC"
                     /locus_tag="ACHUU3_001812"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR2081898.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6776..8035)
                     /gene="hflK"
                     /locus_tag="ACHUU3_001813"
     CDS             complement(6776..8035)
                     /gene="hflK"
                     /locus_tag="ACHUU3_001813"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR2081899.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8121..9401)
                     /gene="hflX"
                     /locus_tag="ACHUU3_001814"
     CDS             complement(8121..9401)
                     /gene="hflX"
                     /locus_tag="ACHUU3_001814"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR2081900.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9478..9786)
                     /gene="hfq"
                     /locus_tag="ACHUU3_001815"
     CDS             complement(9478..9786)
                     /gene="hfq"
                     /locus_tag="ACHUU3_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR2081901.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9872..10822)
                     /gene="miaA"
                     /locus_tag="ACHUU3_001816"
     CDS             complement(9872..10822)
                     /gene="miaA"
                     /locus_tag="ACHUU3_001816"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR2081902.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10815..12662)
                     /gene="mutL"
                     /locus_tag="ACHUU3_001817"
     CDS             complement(10815..12662)
                     /gene="mutL"
                     /locus_tag="ACHUU3_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR2081903.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12672..14009)
                     /gene="amiB"
                     /locus_tag="ACHUU3_001818"
     CDS             complement(12672..14009)
                     /gene="amiB"
                     /locus_tag="ACHUU3_001818"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR2081904.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSLRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14028..14489)
                     /gene="tsaE"
                     /locus_tag="ACHUU3_001819"
     CDS             complement(14028..14489)
                     /gene="tsaE"
                     /locus_tag="ACHUU3_001819"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR2081905.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14461..16008)
                     /gene="nnr"
                     /locus_tag="ACHUU3_001820"
     CDS             complement(14461..16008)
                     /gene="nnr"
                     /locus_tag="ACHUU3_001820"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR2081906.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16007..17146
                     /gene="queG"
                     /locus_tag="ACHUU3_001821"
     CDS             16007..17146
                     /gene="queG"
                     /locus_tag="ACHUU3_001821"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR2081907.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17129..17182)
                     /locus_tag="ACHUU3_001822"
     CDS             complement(17129..17182)
                     /locus_tag="ACHUU3_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2081908.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17416..17491)
                     /locus_tag="ACHUU3_001823"
     tRNA            complement(17416..17491)
                     /locus_tag="ACHUU3_001823"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17456..17458),aa:Gly,seq:gcc)
     gene            complement(17527..17602)
                     /locus_tag="ACHUU3_001824"
     tRNA            complement(17527..17602)
                     /locus_tag="ACHUU3_001824"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17567..17569),aa:Gly,seq:gcc)
     gene            complement(17760..17835)
                     /locus_tag="ACHUU3_001825"
     tRNA            complement(17760..17835)
                     /locus_tag="ACHUU3_001825"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17800..17802),aa:Gly,seq:gcc)
     gene            complement(18046..18591)
                     /gene="orn"
                     /locus_tag="ACHUU3_001826"
     CDS             complement(18046..18591)
                     /gene="orn"
                     /locus_tag="ACHUU3_001826"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR2081909.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18686..19738
                     /gene="rsgA"
                     /locus_tag="ACHUU3_001827"
     CDS             18686..19738
                     /gene="rsgA"
                     /locus_tag="ACHUU3_001827"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR2081910.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19835..20803
                     /gene="asd"
                     /locus_tag="ACHUU3_001828"
     CDS             19835..20803
                     /gene="asd"
                     /locus_tag="ACHUU3_001828"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR2081911.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20825..24148
                     /gene="mscM"
                     /locus_tag="ACHUU3_001829"
     CDS             20825..24148
                     /gene="mscM"
                     /locus_tag="ACHUU3_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR2081912.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24299..>25638)
                     /gene="yjeM"
                     /locus_tag="ACHUU3_001830"
     CDS             complement(24299..>25638)
                     /gene="yjeM"
                     /locus_tag="ACHUU3_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR2081913.1"
                     /translation="MMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWM
                     VSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIA
                     RITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVF
                     AIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLS
                     NGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTL
                     CYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAF
                     FNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVTF
                     ANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5929 a         7192 c         6501 g         6016 t
ORIGIN      
        1 attatcttac cagctataga caaaaaaacc atccaaatct ggatggcttt tcataattca
       61 gacgaactag ctgcgctgac gaaccgcttc aaataagcaa attccggttg caaccgaaac
      121 gttcagggaa gaaacacttc ctgccattgg gatgctgatc aactcatcgc aatgttcacg
      181 ggtcaggcga cgcatacctt caccttccgc gcccatcacc agcgccaagc gtccggtcat
      241 tttgctctga tagagtgtat gatccgcctc gcctgccgta ccgacgatcc agatattctc
      301 ttcctgcaac atacgcatgg tgcgcgcaag gttagtcacc cgaatcagtg gaacgctttc
      361 tgccgcgccg caggctactt ttttcgccgt agcgttgagc tgtgcggagc gatctttcgg
      421 cacaatcacc gcgtgaacgc cagcagcgtc cgcgctacgc aggcacgcgc cgaggttgtg
      481 cggatcggtt acaccgtcga ggatcagcag gaacggttga tcgagcgaag cgatcagatc
      541 cggcagatcg ttttcctgat actgacgtcc tggcttcacg cgggcgataa tgccctgatg
      601 cacggcaccg tcgcttttct cgtcgagata ttggcggttt gccaactgga taaccacgcc
      661 ctgagactca agggcgtgaa tcagcggtaa cagacgttta tcttcacggc cttttaaaat
      721 aaagacttcc tgaaaacgtt caggggcgcg ctccagcagg gcctgcactg cgtggatgcc
      781 gtaaatcatt tcgctcatta atgtactcgt tgtttacgtt ttgcctgatg cactacgttt
      841 atccggccta catgatctct gcaatatatt gaatttgcgt gattatgtag gccggataag
      901 gcgttcacgc cgcatccggc atgaacaaag cgcattttgt cagcaatcta accctcttct
      961 tttaaagagg gtattgatca ctctgccact tttttcttcg ccgcacgctt cgctttggtc
     1021 gctgcggcta ttttctgcgt tttcgccgat ggctttttcg cttttctcgc gtctttcttc
     1081 gccgctttcg gcttcgtttt tttctcaccg cggaaggcgc tgtctggctc aaagtttacc
     1141 tttttaccga cctgacgacg cttgccgcct tttttgcctg catcgccttt tttcgctttc
     1201 tcgcgcgccg ttttaccgac gttgcgcggt gcgcgttcac tggagatcag actaaagtcg
     1261 attttgcgct cgtccatatt aaccgcttcg acgcgaactt ccacgcgatc gcccaggcga
     1321 taagtctggc cgctggattc ccccatcagg cgttgcccta cctggtcaaa gcgatagtag
     1381 tcattgtcca gcgaagagac atggaccaga ccatcaatga acaagtcgtc cagacggacg
     1441 aagaagccaa agccagtgac gctggaaatt acgcctttaa agacgttacc tacctggtcg
     1501 agcatgaagt cacacttcag ccagtcagcg acatcgcgcg ttgcttcgtc ggcacgacgt
     1561 tccgccatcg aacagtgctg acccagttgc aacatctctt ccatcgaata atggtagccg
     1621 ccggtttcag tggtgttgcc ctgatgcccc tgctctttcg ccagcagata cttaatggcg
     1681 cggtgcagcg tcaggtcagg ataacgacga atcggcgaag taaagtgcgc ataggactgc
     1741 aatgccaggc caaagtgacc acggttttct ggatcgtaaa tcgcctgttt catcgagcgc
     1801 agcagcatgg tttgcagcat ttctgcatca ggacggtcgg caaccgactc cagcagttcc
     1861 gcgtagtcac gcggttccgg cttgttacca cccggcagct ccagccccag ctccgccagc
     1921 actgaacgga aagaggtaat cgcttcggtg ctcggcttgt cgtgaatacg gaacagtgcc
     1981 ggttctttcg ctttctcaac gaaacgcgcc gccgagatat tcgccagaat catgcactct
     2041 tcaattaatt tgtgcgcgtc gttacgctgg gtctgttcga tacgttcaat acggcgttca
     2101 gcgttgaaaa tgaacttcgc ttcttcgctc tcaaatgaga tcccaccgcg ttcttcacgg
     2161 gctttatcca gcactttata gaggttatgc aactcttcga gatgcttaac cagcggggcg
     2221 tactgctcgc gcagatcctg atcgccctgc agaatatgcc agactttggt gtaggtcaga
     2281 cgcgcgtgag agctcatcac cgcttcgtag aatttgtagc ccgtcaggcg gcctttcgac
     2341 gaaaccgtca tctcgcacac catacacagg cggtctacct gcgggttgag cgaacacagg
     2401 ccgttagaga gcacttccgg cagcatcggg ataacctgcg aagggaagta caccgacgtg
     2461 ccacggttac gcgcttctct gtccagcggc gttggcggac gcacatagta gctgacgtcg
     2521 gcaatcgcga cccataaacg ccagccgccg ccgcgttttt tctcgcagta aactgcatcg
     2581 tcaaagtcac gggcgtcttc gccatcaatg gtgaccagcg gtaaatcgcg caaatcgaca
     2641 cggcccgctt ttgcttcttc cggcacttct tctttcagcc ccgcaacctg ttgctcaaca
     2701 gcctgcggcc agatatacgg aatttcatgg gtacgcagag cgatatcaac cgccatgccg
     2761 gtgcccatat tgtcgcccag cacttcgacg attttaccca ccgctttggt gcggcgagtc
     2821 ggacgctgag tcagttcgac tacgaccaca aagcccatcc gcgcgcccat gatctgatcg
     2881 ggcgggatta agatatcgaa gctcagacgg ctatcgtcag gaaccacaaa gccgacgccc
     2941 gcttcggtaa agtagcgacc aacaatctgg ctggtttttg gcaccagtac gcggacaata
     3001 cgcgcttcac gacgaccttt acggtcagca cccagcggct gcgccagcac ctgatcgcca
     3061 tgaatacagg ttttcatctg ctcgctggag agatacaaat catctttacg cccttcaacc
     3121 cgcagaaagc cgtagccatc acggtggcca ataacggtac ctttcaccag gtcgaggcgt
     3181 tccggcagcg catagcactg acgacgagtg aagaccagtt gaccatcgcg ctccatcgcg
     3241 cgcaggcggc gacgcaggcc ttcaagctgc tcttcgcctt caatgtgcag ttctaccgcc
     3301 agctcatcac ggctggccgg tttttcacgt ttggttaaat gttcgaggat aaattcccga
     3361 ctagggatgg gattcgcgta tttttcagct tcgcgttcct ggaaaggatc ttgtgacatc
     3421 tcggttcctc cgttgtcatc tctgatgaag attttcgtca ctccaccagc aataatttat
     3481 aaagcggttg attctcttca accaaatcgg caagcgtgta gttatccagt tccgtaagaa
     3541 aactttgcac ggccttagaa agcgcctgtt tcaaccgaca ggcaggtgta atgtggcaaa
     3601 actcactgct gcaattcacc agcgataagg gctccagctc gcgcaccaca tcaccaatac
     3661 gtatcgcact cgccggttta cccaggcgaa tgccgccatt ttttccacga acagcagtca
     3721 cgtagccggc acgactaagt tgattgatta ttttgaccat atgattacgg gagacgccgt
     3781 agacgtcagt cacttcagaa atactggtca tccgcccttc tggcaatgac gccatgtaga
     3841 tcagcgcacg taatccgtaa tcagtgaaac tcgttaactg cacatcaacc tcaaaaggga
     3901 aatcgggaaa aaatacattt atattgatga taaaccagcc acaagctgtg tcgctaattt
     3961 atttcagttc ggggaaggaa aaaagcgagg atttaacact gtgtcggata gcgtaatggc
     4021 ttaaccgaca cagcaatgta tcacactgtt agcccggcag gcaaaatatc tgccaggcgt
     4081 accagaatta cgcgtcgaac ggatcgcgca gaatcatggt ttcagtacga tccggaccgg
     4141 tagagatgat atcgatcggc acaccggtca gctcttcaat acgcttgatg tagttcagcg
     4201 ccgcctgcgg caggccgcta cgatctttca cgccgaaagt ggattcagac cagcccggca
     4261 tggtttcgta aatcggctct acacctttcc agtcgtcagc tgccagcgga gtggtagcca
     4321 cttcgcgacc atccggcata cggtaagcca cgcagagttt aacctctttc aggccatcca
     4381 gaacgtccag cttggtcagg cagaagccag acagggagtt cagctgtacc gcacgacgaa
     4441 cggcaacggt gtccagccag ccggtacgac gacgacgacc cgtagttgcg ccgaattcgt
     4501 taccctgctt gcagaggaac tcgccagttt catcaaacag ttcggtcggg aacggacctg
     4561 cacctacacg agttgagtaa gctttgagga tacccagaac ataatcaaca taacgcgggc
     4621 ccaggccgga accggtcgcc acgccaccag cagtggtgtt ggaagaagtt acgtacggat
     4681 aagtaccgtg gtcgatatcc agcagcgtac cctgcgcacc ttcaaacatg acgaaatcgc
     4741 cacgctgacg cgcctggtcg agcaggtcag aaacgtcaac caccatagaa gtcaggatgt
     4801 cggcaacagc catcgtatca tccagaactt tctggtaatc aaccgcttca gctttgtagt
     4861 agttaaccaa ctggaagttg tgatattcca tcacttcttt cagtttttca gcgaaggttt
     4921 ctttgtcgaa aaggtcgcca acacgcagac cgcgacgtgc tactttatct tcataagcag
     4981 gcccgatacc acgaccggtg gtgccgatcg ctttcgcgcc acgcgctttc tcacgcgcgt
     5041 tatccagcgc aacgtgataa tcaaggatca gcggacatgc ttcagacagc agcagacgct
     5101 cacgaacggg gatgccacgg tcttccagtt cttttatctc tttcatcagc gcggccggag
     5161 acagcacaac accgttaccg atgatgctgg ttacattctc gcggagaata cctgatggaa
     5221 taagatggag aacggttttt tcaccgttga ttacgagagt atggcctgcg ttgtgaccgc
     5281 cctggtagcg tacaacatat ttagcccgtt cagtcagaag atcgacgatc ttacctttac
     5341 cttcgtcacc ccattgggtg cccagtacga cgacgttgtt acccattttt caaaatcacc
     5401 gtttgcttaa aaatggattc taccatcgct ttttcagagt tacagcactt tttgcactca
     5461 aaaatgacct cgatcagtct gttttttgtt cagccaatcg ttttcctcaa catgtagtag
     5521 accacaacgc ccgcaaccac aagtccaccg ccaaaacgac gtaaaatatt atcgggcaaa
     5581 ttggtcatcg cagagatcat cttcttccat gccttcgggt aaagcatcgg ccctaaacct
     5641 tccagtacca aaaccagggc aagcgccagc cagattgtcg aattcattca ttatccttat
     5701 agaaaaagaa aaccaccgac atccctgagg atgcggtggc tttattgacc tgtaccgcag
     5761 tcgttatatt aacgcgttgc ggaagtcggc gtcttcatgt agcggaagaa atcgctatcc
     5821 gggctcatga ccatcacgtc ctgattgcca gagaagctgt tctcataagc acgcaggcta
     5881 cggatgaatg cgtagaagtc cggatcttta ctgaatgcat cagcaaacag tttggctgct
     5941 tcggcatcgc cttcaccacg catgatgcgg ccctgacgct cagcttctgc cagcgttctg
     6001 gtcacttcat agtcggcagt cgcgcgcagt ttttccgctt cttcctgacc ttgtgaacgg
     6061 tgacgacgcg ctaccgcttc acgctcggcg cgcatacggt tgtagatcgc ttcagacact
     6121 tcggtcggca ggttgatctg cttgatacgc acatcgacaa cttcaatacc cagcgccgcc
     6181 atactgttcg ggttgatgac cggaactttg cccttcgtct cagccgttac gcgctctgcc
     6241 gcttcggcaa tggcgttatc tgccgccggg gtagtaactt catcttctgt acccgcagaa
     6301 ccggagttca gcgcgtcacg tacttcgagg gtcagacgac cacgggaatc ggtgacgatg
     6361 tctttcacgt ccaggcgacc aatttcagaa cgcagacggt cagagaactt acgtttcaac
     6421 agcacttccg cttgcgaaat gtcgccacca cccgttgcca ggtagtaacg gctgaaatcg
     6481 ctgatgcgcc atttgatgta agaatcgacg atcaggtctt tcttctcttt ggtcacaaag
     6541 cggtcggcct ggttgtccat ggtctgaata cgtgcgtcga gcattttcac cgtttcaatg
     6601 aacggtatct tgaaatgcag acccggctca taaaccagag gtttgttgtc atcgtcacgc
     6661 agtaccttac caaaacgcag cgtaataccg cgctcacctt ctttgacgac aaagacagac
     6721 atgtaaagca ctaccagcac gatgatgata atcgcgataa ctgacttacg catcgttatt
     6781 ccccctgacg ctggtagtcg ttacgctgcg cgttggcgcg gcgttggtcc ataatatcgc
     6841 cctgactggt ggacgacgtg ttgcttgctc cactggttgt ggaggaagag gctggcggca
     6901 gacgcagcag attgctggca ccgttatcgc tcttcgccgc aggggcgtta ccgcctttca
     6961 gcatctggtc taacggcaga accatcaggt tgccaccttt atcgttaacc agcactttgc
     7021 gggtgttacc caacactttt tccatcgtct cgatatacag acgctcgcga gtaatttccg
     7081 gcgcggcttt atattccggc agaagtttag caaagcgcgc cacttcaccc tgagcttcca
     7141 ggatggtctg ggccttgtac gcacgcgcct cttcgaggat acgttgcgcc tgaccgttcg
     7201 cacgcggctg aacttcgttg gtatacgctt ctgcttcacg aatgtattgc tgttcgtttt
     7261 cacgcgcggc aatcgcatcg tcaaacgccg cttttacttc ttccggcgga cgagctgcct
     7321 ggaagttgac gtccagcaga gtgataccca tgtcatacgg acggatagtc tcttccagtt
     7381 cgcgctgagt atcgctacga atcacggtac gaccttccgt cagaatgcgg tccatggtgt
     7441 atttaccgat aactccacgc agggcgctgt cggtagcctg acgcaggctg tcatccgggc
     7501 tggtcacgct atacagatat ttttccggat tggtgacgcg gtactgcacg ttcatctcaa
     7561 cgcgcactac gttctcgtcc gacgtcagca tcacaccaga agcggccagt tcacgcacgg
     7621 cttccacgtt caccggtttg acttcgtcga taaacgtcgg tttccagttc agacccggct
     7681 caaccagatg gctgaattta ccaaagcgtg ttaccacgcc gcgttcggct tctttaatgg
     7741 tatagaaacc actggccgcc cagataatga caatcgctgc cgctgcgatg gtaacgacac
     7801 gaccgccaag ctgcgggcgc gggccttgcg atgaactgcc accgccagat ccggtgcctt
     7861 taccgccgcc cagaccaccg agctttttgc tcagtttgcg gaagatatca tctaaatcag
     7921 gtggcccttg atcgcgaccg cctttgtttc catttccctc agagttgccg ccaggtttgc
     7981 tgcttcccca cgggtcgcgg tcttgtccgt tattaccggg ctgattccac gccatgtttg
     8041 tgctccatat ttgttatgcg gtgatccctg ttgtctttcg acgccagagg atatgactcc
     8101 acgcttcaga cgctacgccg ttagatcagg taatcgatca acgccggttc ttgtttacag
     8161 aggcgacgcc agtcaacgat cggcatacga acttgcagac ttacgctgcc gtcctcctcc
     8221 atccactctt tttctattgc ctgaagctga taaaaacgac ttctcagacg tccttcctgc
     8281 ggcggcaaac gcaatgtatg ctgcgccacc tcgccggaaa gccgctccgt caaagcctga
     8341 aaaagctgtg gtatccccgc tccggtctgt gcggaaagcc agacacggat cggtttgttc
     8401 tcttcgtccc gatcaatacg cggttcgaaa tcttccagca tatcgatctt gttcatcacc
     8461 agcagggttg ggatctcgtg agcgtcgatc tcttcaagaa ccgtattcac cgcttcgatg
     8521 ttttcttgta cacgtacatc cgccgcatca atgacgtgca gcagtaatgt ggcttgccgc
     8581 gtctcttgta acgtggcttt aaatgctgcc accagatcgt gcggcaggtg gcgaataaac
     8641 cctacggtat ctgcaagtac ggtttcaccg acatccgcga cgtcaatacg ccgcaacgtc
     8701 gggtcgaggg tggcaaacaa ctggtctgcc gcgtagaccc gcgcttcggt gatgcgattg
     8761 aaaagggtag atttaccggc gttggtatat cccaccagcg aaacagtagg aacgtcggct
     8821 ttgatacgcg attgccgccc ctgctcacgc tgcttttcaa ctctttctag gcgcgactgt
     8881 atctgcacga tgcgattacg caacaaacga cggtcggttt cgagctgggt ttcacccgga
     8941 ccacgcaaac ctatcccgcc tttctgtctt tcaaggtggg tccagccacg caccaggcgc
     9001 gtagccagat ggcgcagctg cgccagctca acctgcaact taccctcatg ggtacgcgca
     9061 cgttgggcga aaatatctaa aataaggccg gtgcggtcga taacacgaca ctcgcacaaa
     9121 cgctccaggt tacgctcttg cgccgggctc agggcatggt caaaaagaac gaccgaagca
     9181 cccgtcgctt tgacagcttc cgcaatttca actgctttac cttcacctac aaaatacttt
     9241 gggtgcggcg ctttacggct accggtaatc acctgcaatg cttcgacacc ggcggaagag
     9301 accagagatt caaactcctg gaggtcttcc atatctttgt cttgcgtaaa atagatgtgt
     9361 accagtaccg cctgctcacc agcatcataa cggtcaaaca agcgtataac cctctaaata
     9421 gatcagcggg gaacgcagga tcgctggctc cccgtgtaaa aaaaacagcc cgaaacctta
     9481 ttcggtttct tcgctgtcct gttgcgcgga agtattctgc gcgctgctac catgatggta
     9541 gttactgctg gtaccgccac cggcgttgtt actgtgatga gaaaccgggc gagacgggac
     9601 aacagtagaa atcgcgtgct tgtaaaccat ctggctgacc gtgtttttca acaggatcac
     9661 gaactgatca aaagactcga tttgcccttg cagcttaata ccattcacca aataaataga
     9721 aactggaaca cgttcccgac gcagtgcgtt caggaacgga tcttgtaaag attgcccctt
     9781 agccattctc tcttttcctt atatgcttat ttgtactttg aacctttcga ttctgaaaaa
     9841 attgcgcacg atacgtctca attgtacaca ttcagcctgc gatagcacca acaacctgta
     9901 atacttcgtc acgcgcctgt tctggttttt cactgtcaag ccagtgaacc ccttcccaac
     9961 cacgcagcca ggttatctgc cgcttcgcca actgtctcgt ggcgcaaaca cctcgataaa
    10021 ccatttcatc gtatgagatt tcgccttcaa ggtaagacca catctggcga taacccacgc
    10081 aacgaatgga aggcaagtcc gtatgcaaat ctcctcgggc aaaaagcgcc cggacttctg
    10141 cttcaaaacc tgaagccaac atctgatgaa aacgctgctc aatgcgttga tggagcagtt
    10201 cacggctcgc cggggcgatg gcgaactgat gcacctgata cggtagagcg tctcctgacg
    10261 tttgcgtcag ttccgttaaa gttttacccg aaatgaaaaa aacttccagt gcccgggaaa
    10321 gcctttgtgg atcatttgga tgaatccttg ctgccgcaac cggatctacc tcctgaagtt
    10381 gacgatgcaa tgactcccaa ccttgctctg ccgcctgttg ctcaattctg gcccgcactt
    10441 ccgggtctgc cgacggtagc ggcgacaacc cttccagcaa tgccttgaaa tacaacatcg
    10501 taccgcccac taacagtggg atccgccccg ccgcggtgat atcggccatt tccgccagcg
    10561 catcgcggcg aaaatcagca gccgagtaag cctgcgacgg atcgcgaata tccagcaatc
    10621 ggtgcggcgc ggcgagtaac tcttcagcgt tcggcttcgc cgtcccgata tccatccctt
    10681 tgtaaataag ggcagaatca acgcttatca actctactgg taaaatttta cgcagctcaa
    10741 tggctaacgc cgttttaccg gaggccgtcg gccccatcaa aaaaatcgcc ttaggcaggc
    10801 tcgccttact gatatcactc atctttcagg gcttttatcg ccggatgtaa atcaacagat
    10861 tgtaacagac cacccggcgg cgttttcaca agttgcggac ataaccgttc cacgtccgcc
    10921 agcagggtta tggcctgtgc cattgaccac tgcgcatgtt cgctcatcag atttcgtgca
    10981 atccactgcg caatattgcc aggttcgaat acggactgct tcgccaggta gcctatcagt
    11041 tcaggaatca agatttgtaa attttgttgg cgtaagggta aaggcacggc cctgatggtc
    11101 acatgctgtg catctgactg gaaatcaata cccaattccg ccagggcaga ctgcgctttt
    11161 tctaatgccg atttttcttc gccagaaact tttagccgca acggaataag cagcggctga
    11221 gcgcaaactg gcgcttcacc cggcgtcagt tgtacctgac gcagccaacg ttctgccact
    11281 ggcaaggcta aaagtgaaat gttgccgtcg cgctccagca acgcacagtc ggaatggacg
    11341 atagtcagta cccgaccaaa actctgactg ttcgccgcaa gtgcaggttc ctgcggttcc
    11401 ggcgctttta atttttgcat cggcgcgggc gtttgcaaaa gctggcgata cacttcacct
    11461 tgctgtttct ggtagcctgg ctgcgcattc ggccaggggg cagccggacg actgcctgat
    11521 gctggcgcag gagtgtagcg cggagctacc ggctcacgag ctgccggttc tgcaaagtga
    11581 ttgcgccccg ccgccacgcg gttttccgga atggaacgcg gtgcaggttg gggttcatcg
    11641 tccagcggta gcggcgtttc cagttgctgt tgtagcacgc tcagcacgcc ctgatagata
    11701 aaatcatgca ccagacgcga ctgatggaaa cgcacttcgt gtttggcggg gtgcacgttg
    11761 acgtccacct gatgcggatc gatctccaga tacagcacaa atgccggttg ctgatcggcc
    11821 cccagtttgt cttcgcaggc ctggcggatc gcgtgattga tcaggcgatc gcgcatcatg
    11881 cgaccgttca cgtagcaata ctgaatttct gccagtgcgg gcgtggtgtg atttggatcg
    11941 gccacccagc cgcgtagcgt gagatcgccg tgttgccatt caatcgccag cgcttgttca
    12001 agaaaagcgg tgccgcaaat cgcgcctaag cgccgttctt tttgcccgcc ttccggcact
    12061 gcgcggtact gacgcacaat tttaccgtta tgcgacaggt tgatcgtgac gtcgaaacgc
    12121 gccagcgcaa tgcggcggat gatctcatca atgtggttaa attcggtttt ctcggtgcgc
    12181 aggaatttgc gccgcgccgg ggtgttgtag aacagatcca gcacctccag cgtcgtcccc
    12241 acaggatgcg ccgccggttt taccgtcacg ttcatatcgc gcccttcggc ataggcctgc
    12301 caggcttcct gctgttctgc ggtgcgtgaa gtgagcgtca ggcgggaaac cgaactgata
    12361 ctcgccagcg cctcaccgcg aaagcccagg ctgataatgg cttcgagatc gtccagagag
    12421 gcgattttac tggtggcatg acgagccagc gccagcgcca gctcatcttt tttgataccg
    12481 cagccgttat cacgaatgcg gataagtttc gccccaccgc gttcgatatc aatatcgata
    12541 cgcgtcgcac ctgcatcgag gctgttttcc accagttctt tgactaccga cgcaggtcgc
    12601 tcgaccacct cacctgcggc aatctggttc gccagttgtg gcggtaagac ctgaattggc
    12661 atcaatcgtc cttagtttgg cagcgtgcga tctggcgtcg tcaccgtact ggcagtttgt
    12721 gccgttgccc cctgcggcgc agattgcatc ggatgcgcaa ggaaataatt gcgcaggcct
    12781 ttgtaaatgg cttctgccag ctgttgttgg taatcgtcgc tcgccagcaa acgttcttcg
    12841 ctgttgttgc tgataaaacc ggtttcgacc agtactgacg ggatatccgg cgaacgcaga
    12901 acgccgagac tcgcgtgttc tggtcggcgt ttatgtattt cgccaatgcg ttgcaactga
    12961 ctgatcatac tggtcgctac atcataccct acccgcaggg aatgaccgaa ctgtaaatcc
    13021 agcaccgcct ggcttaaata ggggtcagac tgactgttcg ccagcacatc gcccgcccca
    13081 cccagcagct ccgactgttt ctcgtgctgc tccagccagc tggccatttc gctgttggcg
    13141 cgacggttag agagcaccca tacggaagcg ccagtcgcac tgcggttcgg tgcggcatca
    13201 gcgtgaatcg acacgaggaa attggcgttt tgcttacgtg ccacatcgct gcgccccatc
    13261 accgagataa agtaatcccc gtcacgggtt aaaacgcctt taaacatcgg atcgtcattg
    13321 agcaaagtgc gcaatttacg cgcgatggcg atggtgacat ttttctcccg cgtaccaccg
    13381 gggccgatag cgccagggtc ctgaccgccg tgtccggcat caatagcgat gataatttta
    13441 tcgccagtgt tagccgtcgc gcgcgctgcc ggacgcgtta ccgtattact gctgataaca
    13501 cccgtagtgc ggttactttc cgttttaaac ggattgcgcg ccggttcgct gacgcgcggt
    13561 gcgacaaccg caggcgtttc aacgcgtttc gcaaccacgg gcggtggagg aggcggtggc
    13621 ggcacatcgg cgttaatcgt aaagacgaca gtgtaattgc tgccattctg ccgcttcacc
    13681 gcttcggttt taccgttttc ggtaagatcg accaccagcc gtagcgtttg tgcatcttta
    13741 ggcgttccag agcgaatcgc cttcaccaga ttattgccgc tgaacaacaa cggcagtccc
    13801 tgaatcacgc ccgtttgttt gatatcgagc gccacggtgc gtttgctttg atggctaaac
    13861 gcataatcag gatcgccaat aaaactcaac gttatccgcg cctgttgatt accgttagaa
    13921 acctgaatat cagagagcgt cgcggcaccc accggcgtgc acagcagcag cagcgtcgct
    13981 accaaccaat ttctgatgcg atacatcatc ccgccacctt tcaaaggtta accggctaaa
    14041 cgcgccagca acaattcacc cgcagaggaa accgcactca cgcgcgcctc acggccttgt
    14101 gcctgataat caatgtgtat ttcgacatcc gggtcaggaa gaacacctgt accttgttgt
    14161 ggccactcca ccaggcagat ggcatcgttg gcaaaataat cgcggatccc cataaactcc
    14221 agctcctcgg gatcggcaag gcggtacaaa tcaaagtgat aaaccattaa attgtcgagc
    14281 gtatagggtt cgaccagcgt ataagtgggg cttttgacat taccctgatg acccagagct
    14341 tgtaaaaaac cccggctaaa ggtggtttta cctgcgccta aatcgccata cagatagatt
    14401 acggttgcgc catcgcaggc tttcgctacc cgctcgccca ggtctaatgt tgcctgctca
    14461 tcagggagcg gaattactcg attcatcatg gtttttatca gtcacttccg ggttaacaat
    14521 acgctgtagc gtggaaaaga gatcggttgc cagcatcccg cgcgttccaa aacgcgccgc
    14581 cagtacgtca gctgccgcac cgtgcgcgac acagcccgca caggcggcat catacggcga
    14641 cagtttttgc ccaagcaatg cgccaataat accagagagc acatcgccca tgccgccgct
    14701 cgccatgcct gcatttccgg catcaataat gcctaaagcg tcaggatggg cggcgaccac
    14761 ggttccggca cctttcagca ccgctacgcc gccataacgt tgtaccagac gtttggcgca
    14821 atgtaagcgg tcactttcaa tttcagcgac ggaacagcct aacaaccgtg cggcctcgcc
    14881 aggatgcggc gtgatcacgc gattgtgacg cttatcggga ttgattgcca gcaggttcaa
    14941 tgcatcggca tcccacaaca tcggtttgcg aaaattctca actttttgca gtgctttttt
    15001 cccccactct tgctggccca gaccgggacc aatcaccacc acatcggccc attccaggct
    15061 ttcggtaaga gagtccatcg tcagttcatg caccatcaat tccggtcgtg cagtcagcag
    15121 cggcgcaatg ttttcactgc gggtcagtac tcggactaaa ccagcaccag cacgcagcgc
    15181 cgcttccccc gtcatacgaa tagcccccgc cgtgccgtga tcgccaccga taatcaccag
    15241 ccgcccgtga tcgcctttat gcgaagtcgg gcgacgcggt ttcagccagt gagaaagttg
    15301 ttctgccgaa aaccgctgaa ttttcgtctc ctgacctgcc agccagctat ccagccccag
    15361 tgagtcaaaa tgcagttgtc ctgtaacatc ccgcgctttt ccagtgagca agcctggttt
    15421 cagcgcaata aaagtgatgg tgtgatcggc gttgatcact gcgcctggcg tagcgccggt
    15481 ttcagccagc aggccggaag ggatatcaac cgccacaatc ggcgcaggat gggaattagc
    15541 gtggtcgatt aactggctaa tggattcgcg gggcgcttgc tgcaagccgg taccgagcag
    15601 cgcatcaaca atcagatcta ccgattcggg ccagacaata ttcgaagcat ggatctcgcc
    15661 acccgcgttt aaccatgctt cgcgtgccag cgcggcctct tccggcaacg gtttgtcgct
    15721 ctcctgggcc aacaacgtga cctcaatgcc gaccgctttg gccagtcgcg cgaccacgta
    15781 gccatcgccg ccgttattac catgaccgca cagcaccagc cagtggcggg cgtcaggata
    15841 cgccgaacga cacacctgga atgcggcctc gccagcgcga agcatcagct catagagtgt
    15901 gagccccagc acatctgccg cctcgcgttc tccgcggcgg atatcgtcgg cgtaccagac
    15961 ggtgtgtggt atacttacgg ggtttttctt cattgtatgg tccgtcatgt cagagcccct
    16021 cgatctcaat cagttagcgc aaaaaattaa acagtggggg ctggaactgg gctttcagca
    16081 ggtaggtatt accgataccg atctcagcga gtccgagccc aaactgcaag catggctgga
    16141 caaacaatac cacggcgaaa tggactggat ggcacgtcac ggtatgctgc gcgctcgccc
    16201 ccatgagtta ttaccaggta cgctgcgtgt aatcagcgtg cggatgaatt accttcctgc
    16261 taacgccgca tttgccagca cgctgaaaaa ccccaaactc ggctatgtta gccgttatgc
    16321 gctgggccgt gactatcaca aacttctgcg caaccgactc aaaaagctgg gcgagatgat
    16381 tcagcaacat tgtgtttcgc tgaattttag accgtttgtc gattctgcgc ctattctcga
    16441 gcgcccgtta gctgaaaaag ctgggctcgg ctggacaggt aagcactcac ttatcctcaa
    16501 tcgcgaggcc ggttctttct tctttttagg cgaattgctg gtcgatattc cgctgcccgt
    16561 ggatcaacca gtcgaggaag gatgcggtaa atgcatagcc tgtatgacga tttgcccgac
    16621 cggtgccatc gtcgagccat ataccgtcga tgctcgccgc tgtatctctt atctcaccat
    16681 cgaacttgaa ggggcgatcc cggaagagtt gcgaccgtta atgggaaacc gtatttacgg
    16741 ttgcgatgac tgccagctta tctgcccgtg gaatcgctat tcgcaactca ctacagaaga
    16801 cgatttcagc ccgcgtaagc cgctacacgc acaggaactc attgagttat ttgcctggag
    16861 cgaagagaag tttttaaaag tcacggaagg atcggcgatt cgccgtatag gtcacctgcg
    16921 ctggctgcgt aatatcgccg tggcattagg caatgcgccc tgggatgaaa cgattctggc
    16981 ggcgctggag agtcgcaaag gtgagcaccc acttcttgat gagcacatag cgtgggcgat
    17041 tgcgcagcaa atcgagagac gaaatgcgtg catagtcgaa gtgcaattgc cgaaaaaaca
    17101 gcgtctggtt cgggtgattg aaaaagggtt accgcgtgac gcctgattca tccacagctt
    17161 gtgaataaaa ataaaaacgc attgcaattc aagacgcata aaatcgtcaa gtgatcgact
    17221 cgacaaaaca gaatcttgat ttttaactta tttatcaata ggttaaaaaa ataccattca
    17281 ccgtgcgaag tttctttgaa attaagatta cagcctgagg atgtggataa ctctgtttac
    17341 aagagtttgt cagagacaac aaaaaccagc aataacgcat cgcgtcgctg tggatatttt
    17401 attgagagaa gaatttggag cgggaaacga gactcgaact cgcgaccccg accttggcaa
    17461 ggtcgtgctc taccaactga gctattcccg cttgggtggt ctgtgcctta cagcactttc
    17521 aaattttgga gcgggaaacg agactcgaac tcgcgacccc gaccttggca aggtcgtgct
    17581 ctaccaactg agctattccc gcttaatctt cgtctttcga accgctactg ccttggctgc
    17641 cttcactcac cttagtcaca tacttttgta agttcccaag gattcgttca gttgccgcct
    17701 tgtcgcaatt cgaaatactg cgattgggtg gtctgtgctt tgcagcactt ttcaaatttt
    17761 ggagcgggaa acgagactcg aactcgcgac cccgaccttg gcaaggtcgt gctctaccaa
    17821 ctgagctatt cccgcgtact acttaatttt gctttcgtaa ttttcgcatt tctgcgtcgt
    17881 tacgggaggc gcattatacg agaaatcttt ccctctgcaa ccccccaaaa aagttttttt
    17941 tgcgttttta tctcaagtgc tgaaattatc agcaagacgt tcaatttagc gtcaaaatca
    18001 cgccgccgca agtggattcc ggcgcttacg tgaccaggaa aaattttaca gcttgataaa
    18061 atgctcgcgg tagtaagcca gctccgccac cgattcacgg atatcatcca ttgcctgatg
    18121 cgtcccctgc ttggtaaaac catccagaat ttccggcttc cagcggcgcg ccagctcttt
    18181 cagggtgctg acatcgagat aacggtagtg gaagtaggct tccagctccg gcatgtattt
    18241 aaacaggaaa cgacggtcct gtccgatgct gttaccgcaa atcggcgatt ttcccgcagg
    18301 cacccactgt tttaagaatt cgagcgttgc cagttcagct tcccgatcgc ccatcgtgct
    18361 cgctttcacg cgctctacca gcccgctggc ggtatgggtg cgcacgttcc agtcatccat
    18421 cagcgccagc tgttcatcgg actgatgtac tgcaatggtc ggcccttctg ccagaatatt
    18481 cagattggca tcggtcacca gcgtggcaat ctcaataatg cgatcgcgct cgggatccag
    18541 accggtcatc tcaagatcga tccaaatcag gttgttttca ttggcactca tgctattttc
    18601 cacccattca cagtgactat attgatccac aattgcgtgt atcatagatg ttttgcccat
    18661 caggggcgac caggagtcag tacgattgag taaaaataaa ctctccaaag gccagcagcg
    18721 ccgcgtgaac gccaatcacc agcgtcgtct taaaacgtct aaggagaagc ccgactacga
    18781 cgacaatctg tttggcgagc ctgatgaagg tatcgtcatc agccgctttg gtatgcacgc
    18841 tgatgtggaa tccgccgatg gcgacgttca ccgctgcaat attcgccgta ccatccgttc
    18901 gctggtaacc ggcgaccgcg tagtctggcg tccgggtaaa ccggcggcgg aaggcgtaaa
    18961 tgtcaaaggg atcgtggaag cggtgcatga gcgtacctcg gtgttgacgc gtccggattt
    19021 ctacgacggc gtaaaaccta ttgccgccaa catcgaccag attgtcattg tctccgccat
    19081 tttgccggag ctgtcgctca atattatcga ccgttacctg gtggcctgcg aaaccttgca
    19141 gattgagccg attattgtgc tcaacaagat agacctgctg gacgacgaag gcatggcgtt
    19201 cgtcaacgag cagatggata tctaccgcaa tatcggttat cgcgtattga tggtttccag
    19261 ccatactcag gatgggctaa aaccgctgga agaggcgttg accgggcgca tcagcatttt
    19321 tgccgggcag tctggcgtcg gcaaatccag cctgctgaac gcgttgctgg ggctgcaaaa
    19381 agagatcctg accaacgatg tctctgacaa ctcggggctt ggtcagcaca ccacgaccgc
    19441 cgctcggctg tatcacttcc cgcacggcgg tgatgtgatt gattcccctg gtgtgcgtga
    19501 gttcggcctc tggcacctgg agccggaaca aatcactcag ggctttgtcg aattccatga
    19561 ctatttaggt ctgtgtaaat atcgcgattg caaacacgat accgatccgg gctgcgctat
    19621 ccgggaagcg gttgaggaag ggaaaatcgc ggaaacccgt ttcgaaaact atcaccgtat
    19681 tctggaaagc atggcgcagg taaaaacgcg taaaaacttt tctgatacgg atgactgaca
    19741 attaagctaa ccgtcgttag aatcgtcccc tttttttcag gatccggcat gtaggccgaa
    19801 tcaggaacga caaaacaatg gcctggaggc taccttgtta aattcattta aactttcgct
    19861 acagtacatt ctgccgaaac tatggcttac tcgcctggcg ggttggggcg caagcaagcg
    19921 ggcaggatgg ttgacaaaac tggttatcga tctgttcgtt aaatactaca aggtcgacat
    19981 gaaagaggcg caaaagccgg acaccgccag ctaccgcacc tttaacgaat tctttgtccg
    20041 tccgctgcgt gacgaagtac gcccaatcga taccgatccg aatgtactgg tcatgcctgc
    20101 cgatggcgtt atcagccagt tgggtaaaat cgaagaagat aaaatcctgc aagccaaagg
    20161 ccacaactac agcctcgaag ccctgctggc aggcaactat ctgatggcgg acctgttccg
    20221 caacggtacg tttgtgacca cttacctctc cccgcgtgac taccaccgcg tacacatgcc
    20281 gtgcaacggt attctgcgtg agatgatcta cgtgccgggc gatctcttct ccgttaacca
    20341 tctcacggct cagaacgtgc cgaatctgtt tgcccgtaac gaacgcgtga tttgcctttt
    20401 cgataccgaa tttggcccaa tggcgcagat tctggtcgga gcgacgattg ttggcagcat
    20461 tgagacggtc tgggcgggca ccattacgcc gccgcgcgaa ggtatcatca agcgctggac
    20521 ctggcctgcc ggggaaaacg acggttctgt ggcactgctg aaaggccagg aaatgggtcg
    20581 ctttaaactc ggttccaccg ttatcaacct gtttgcaccg ggtaaagtga atctggttga
    20641 gcaactggaa agcctgtctg tcacgaaaat ttgccagccg ctggcagtat ctaccgaaac
    20701 ctttgttacg ccagacgctg aacctgcccc gcttcctgct gaagagatcg aagcagaaca
    20761 cgacgccagc ccattggttg acgacaaaaa agaccaggtc taatccatca aaggaaacgc
    20821 tgacgtgcgc ctgattatca cttttctgat ggcctggtgc ctcagttggg gggcgtacgc
    20881 cgcgacggcc cccgatagca aacaaatcac tcaggaactg gagcaggcaa aagcggcgaa
    20941 acccgcacag ccggaagtcg tagaggcgct tcagtctgcc ttaaatgcgc ttgaggaacg
    21001 aaaaggttcc cttgagcgca tcaaacaata tcagcaagtt atcgataatt atccgaaact
    21061 ctccgctact ctgcgcgcac aattaaacaa catgcgtgac aagccgcgca gcgtgtcgcc
    21121 gggaatgtct accgacgcgc tgaatcagga aattctccag gtcagcagcc agttgctgga
    21181 taaaagccgt caggcccagc aagagcagga gcgcgcccgc gagattgccg attcgctgaa
    21241 tcaactgccg caacagcaaa ccgacgcccg ccgtcagtta aatgagatcg agcgccgcct
    21301 gggaacgctt accggcaata ctccgctcaa tcaggcacaa aatttcgcgt tgcagtctga
    21361 ctctgcacgt cttaaggcgc tcgttgatga actggagctg gcgcagctgt ctgccaataa
    21421 ccgccaagaa ttagcgcgct tacgctcaga gcttgcggaa aaagagagcc agcaactgga
    21481 tgcgtatttg caggccttac gtaatcagtt gaatagccaa cgtcagcttg aggcggagcg
    21541 ggcgctggaa agtaccgaat tactggcaga aaacagtgcc gatttgccga aagatatcgt
    21601 cgcgcaattc aaaattaacc gcgaactatc ggcagcgctg aatcaacagg cgcagcggat
    21661 ggatctcgtt gcctcgcaac agcgtcaggc tgccagccag acgttacagg tccggcaggc
    21721 gttgaatacg ctgcgtgaac agtcgcaatg gctgggatcg tccaatctgc tcggtgaagc
    21781 gttgcgagcg caggtggcac ggctaccaga aatgccaaaa ccacaacagc ttgataccga
    21841 aatggcgcag ttgcgtgtgc aacggttacg ttatgaggat ctgcttaata aacagccgct
    21901 gctacggcaa atccatcagg ccgacggtca gccgctgacc gccgagcaaa accgtattct
    21961 ggaagcacaa ctgcgcactc agcgtgagtt gctgaactca ttgttgcagg gtggcgacac
    22021 gctactattg gaactgacca agctgaaagt ctccaacggg caactggagg atgcgctgaa
    22081 agaggtgaac gaagcaacgc accgctatct gttctggacc tctgacgtgc gcccgatgac
    22141 catcgcctgg ccgctggaaa tcgcccagga tctgcgtcgt ctcatttcgc tggacacctt
    22201 cagtcagttg ggcaaagcca gtgtgatgat gctgaccagc aaagagacaa ttttgccgct
    22261 gtttggcgcg ttgattctgg tcggttgcag tatttactcg cgccgctatt tcacccgttt
    22321 tcttgaacgt tcggcggcga aagttggcaa agtgactcag gatcacttct ggctgacgtt
    22381 gcgcactctt ttctggtcga ttctcgtcgc gtcaccgtta ccggtgctgt ggatgacgct
    22441 gggttacggc ttgcgcgagg cgtggcctta tccgctggcg gtcgcgattg gcgatggtgt
    22501 aacggccacc gtgccgctgc tgtgggtagt gatgatttgc gccacctttg cccgcccgaa
    22561 cggcttgttt atcgctcatt ttggctggcc gcgcgaacgc gtatctcgtg ggatgcgcta
    22621 ctacctgatg agcatcgggc ttattgtgcc gctgattatg gcgctgatga tgttcgataa
    22681 cctcgacgac cgtgaattct ccggttcgct gggaaggctt tgctttatcc tcatttgcgg
    22741 tgcgctggcg gtggtcaccc tcagcctgaa aaaggccggg attccgctgt atctcaacaa
    22801 agaaggcagc ggcgacaaca ttaccaacca tatgctgtgg aacatgatga ttggcgcgcc
    22861 gttggttgcc attctggcgt cggctgtggg ttatctggca acggcacagg cgctgttagc
    22921 gaggcttgaa acctcggttg ccatctggtt cctgctactg gtggtttatc acgttatccg
    22981 ccgctggatg ctgatccagc gccgcaggct ggcgtttgac cgggcgaagc atcgccgggc
    23041 agagatgtta gcgcaacgtg cgcgtggcga agaggaagcg catcatcaca gtagcccgga
    23101 aggagcaatt gaagtcgatg aaagcgaagt cgatctcgat gccatcagtg cgcaatcctt
    23161 gcggctggtg cgctcaattt tgatgttgat cgccctgctt tctgtcattg tgctgtggtc
    23221 agaaatccat tccgctttcg gcttcctcga aaatatttcg ctgtgggatg tcacctccac
    23281 ggtacagggc gtagaaagtc tggagccaat taccctcggt gcggtgctga ttgccattct
    23341 ggtgtttatc atcaccacgc agctggtgcg caacctgccc gcgctgctgg aactggcgat
    23401 tttgcagcac ctggatttaa cgccgggtac gggctacgcc atcaccacca tcaccaaata
    23461 tctgctgatg ctgattggcg ggctggtcgg cttctcgatg attggtattg agtggtcgaa
    23521 attgcagtgg ctggtcgccg cgctcggtgt tggtctcggt tttggtttgc aggaaatttt
    23581 cgccaacttt atctctggtc tgattatcct gttcgaaaaa ccgattcgca ttggcgatac
    23641 ggtgacaatt cgcgatctca ccggtagcgt gacgaaaatt aacacccgcg ccaccaccat
    23701 cagcgactgg gaccgtaaag agataatcgt gccgaacaag gcgtttatta ccgagcagtt
    23761 tatcaactgg tcgctctctg actcggtcac gcgcgtagtg ttgacgattc cggcccctgc
    23821 tgatgccaac agtgaagaag tgacggaaat cctgctcacc gcagcgcgtc gctgctcgct
    23881 ggtgatcgac aacccggcac cggaagtctt cctggtggat ctgcaacaag ggattcagat
    23941 tttcgagctg cgtatttatg ccgctgagat gggtcaccgt atgccgctac gccatgagat
    24001 ccaccagctg attctggctg gcttccatgc ccacggtatc gatatgccat tcccgccctt
    24061 ccagatgcgt ctggaaagcc tcaacggtaa acaaacgggg agaacgctga cgtctgcggg
    24121 caaaggtcgt caggcgggaa gtttgtaatg catgaagctg ccaaatgatg tcaacgtggc
    24181 tcatttagca gcttaacatc atcttcagtt atcaccggcc ctggagagta tcatccatac
    24241 gcattacttc ctgacaactc atcgccggag agacgatgag ttgcaggatt tcatatgatc
    24301 agtcgagagc taactcaggt ttattcgcca tgcgactgca atagttctgg taaatcgcca
    24361 tcgctaacag cgagaagaag acagggccgc caatcatcca caatgtgctg tcccagtctc
    24421 cggcttcaac cacaggctga ataatggtga agacgttcgc aaatgtcacc accagtacga
    24481 caaccaccat cgcaatcatt gccgacatac gcgttttgaa aatcacaaat ggtctgtcga
    24541 gatcctgacg tgctttaaag aatgggaaag ccagcgcgag gaacaggtaa ggaagcgtca
    24601 tagacacgtt cgccatcagc gtcagcttgt taaagaacgc ggatgcggta ccgccaccaa
    24661 acgaaaccag caggatgaag acagtaacca acccgcactg catccacatg gcgatagacg
    24721 gcatccccat cgcattcagg cgcgtcatcg gttccggcca caacgctttc ggcgtcccct
    24781 ggatgatggc tttcagcggt gaatagcaaa gcgtaaagaa cgcaccggta taagcgagga
    24841 acatcgaaag tccggtaata cgcgcaaacc atacgcccag cgacaatgac gcttcaggtg
    24901 acaaatgcag tgcgttaccc agcgtcaccc cgaggctctt catcagcaca taggtaatat
    24961 tgccaaggtt aacggaacca ttacttaata cctgctgcca gtttgtgctg acgccccata
    25021 aaaatattgc cagcgaataa ccgattgaaa taacaatagc ggcaaaaaca atacctttgg
    25081 caaagttctt ttctggattt tccgttttat cgaccagacc accaaccgct tcaattccgc
    25141 cataggcaaa aatagcaaat accacaaacg atagcattgc cagaccggac tgataacccg
    25201 ggttcggtga tgcaaggaaa ttaatatcct gcgcgaaatg cccaccattt aataacaaaa
    25261 tagtaatgct tactaacagc aatactaaat tcagacacat tactgcaata ccgcccaccg
    25321 cagtaatgcg ggcaatttta ttaatcccct ttgaagcaac gacggtgacc agaatcatcc
    25381 atgctactgc cagcagacca accacctgcg taggctccag tccggcaata cgccagtgct
    25441 gggtcatgtc gctaccatag aggaatgttg agaacggtac ccaaactttc gcggaggtac
    25501 tcaccatcca gatgatataa gaggaaaacc acataaacgt accaatgaag gcaaaacgtg
    25561 gtccgacact attattcatc caggaataga taccaccttc ttctttgcga taagcagctc
    25621 ccatttcagc catcatta
//
LOCUS       DAZPLI010000046        25549 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZPLI010000046 DAZPLI010000000
VERSION     DAZPLI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.046"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..388)
                     /locus_tag="ACHUU3_001831"
     CDS             complement(<1..388)
                     /locus_tag="ACHUU3_001831"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFR2081914.1"
                     /translation="MTVKVIDLFCGAGGLTHGLQLAGLDVVAGIDLEGECRFPYERNN
                     KSKFIEQDIAKVTKEELLRLYGDASVKVLAGCAPCQPFSKYTQGKDKADDKKWPLLYE
                     FERLIREVSPEIVTMENVPDVTKHKVY"
     gene            complement(475..2772)
                     /locus_tag="ACHUU3_001832"
     CDS             complement(475..2772)
                     /locus_tag="ACHUU3_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFR2081915.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASMLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWISQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDSGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDE
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKHGLRRPNRQ
                     QRNGSPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWELEVLARGATVNYDG
                     KKFTYPLADEWSGFSTVLESNSVKR"
     gene            complement(2762..3037)
                     /locus_tag="ACHUU3_001833"
     CDS             complement(2762..3037)
                     /locus_tag="ACHUU3_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFR2081916.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(3034..3258)
                     /locus_tag="ACHUU3_001834"
     CDS             complement(3034..3258)
                     /locus_tag="ACHUU3_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFR2081917.1"
                     /translation="MPDNIDFIQEQQAELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            complement(3261..3560)
                     /locus_tag="ACHUU3_001835"
     CDS             complement(3261..3560)
                     /locus_tag="ACHUU3_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFR2081918.1"
                     /translation="MSCSHSVVLLNNALKIAVMKNGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRS"
     gene            complement(3560..3784)
                     /locus_tag="ACHUU3_001836"
     CDS             complement(3560..3784)
                     /locus_tag="ACHUU3_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFR2081919.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(3848..4348)
                     /locus_tag="ACHUU3_001837"
     CDS             complement(3848..4348)
                     /locus_tag="ACHUU3_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFR2081920.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(4518..4790)
                     /locus_tag="ACHUU3_001838"
     CDS             complement(4518..4790)
                     /locus_tag="ACHUU3_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFR2081921.1"
                     /translation="MDAENYVIQYPLDAVHVDKFADLLGKPKTAVSEMVKANKLPIIE
                     LRDPCKPKARAGEKWVFIPEFNRAVREAFYNRPVEQRDAWLLWMGL"
     gene            4927..5220
                     /locus_tag="ACHUU3_001839"
     CDS             4927..5220
                     /locus_tag="ACHUU3_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR2081922.1"
                     /translation="MKLSEKIKALREAEGLSQSKFCEIIELPLSTLKKYEGGNFEPGG
                     TALLKITMHPTFQKYALWLMTDKTAPDAGQIAPALAHIGPESTESNHSAKRIG"
     gene            5290..6270
                     /locus_tag="ACHUU3_001840"
     CDS             5290..6270
                     /locus_tag="ACHUU3_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR2081923.1"
                     /translation="MAIKKLDDGRYEVDIRPRGRDGKRIRRKFERKAEALAFERYTIA
                     NASQKEWGGQRADRRTLSELLDIWWKYHGQNHEHGTKEFNHLLKTISGIGDIPVSRMS
                     KRALMDYRSMRLRDGISAATINRDMYRLSGMFTKLIQLDEFSGQHPIHGLPPLAEANP
                     EMTFLEKAEIEKLLNVLAGDDLLVALLCLSTGGRWTKVATLKPAQITNCRVTFLKTKN
                     GKKRTVPISEELEKKVKEEASAKLFKVDYEKFCGILRRVKPDIPPNQATHILRHTFAS
                     HFMMNGGNIIALQQILGHASIQQTMAYAHLAPDYLQNAVALNPLKGGVTL"
     gene            complement(6456..6956)
                     /gene="cpxP"
                     /locus_tag="ACHUU3_001841"
     CDS             complement(6456..6956)
                     /gene="cpxP"
                     /locus_tag="ACHUU3_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR2081924.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            7106..7804
                     /gene="cpxR"
                     /locus_tag="ACHUU3_001842"
     CDS             7106..7804
                     /gene="cpxR"
                     /locus_tag="ACHUU3_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR2081925.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            7801..9174
                     /gene="cpxA"
                     /locus_tag="ACHUU3_001843"
     CDS             7801..9174
                     /gene="cpxA"
                     /locus_tag="ACHUU3_001843"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR2081926.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(9280..9954)
                     /gene="yiiM"
                     /locus_tag="ACHUU3_001844"
     CDS             complement(9280..9954)
                     /gene="yiiM"
                     /locus_tag="ACHUU3_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR2081927.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(10103..11086)
                     /gene="kdgT"
                     /locus_tag="ACHUU3_001845"
     CDS             complement(10103..11086)
                     /gene="kdgT"
                     /locus_tag="ACHUU3_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFR2081928.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(11346..11966)
                     /gene="sodA"
                     /locus_tag="ACHUU3_001846"
     CDS             complement(11346..11966)
                     /gene="sodA"
                     /locus_tag="ACHUU3_001846"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR2081929.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            12251..13285
                     /gene="rhaT"
                     /locus_tag="ACHUU3_001847"
     CDS             12251..13285
                     /gene="rhaT"
                     /locus_tag="ACHUU3_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFR2081930.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(13282..14130)
                     /gene="rhaR"
                     /locus_tag="ACHUU3_001848"
     CDS             complement(13282..14130)
                     /gene="rhaR"
                     /locus_tag="ACHUU3_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFR2081931.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(14204..15040)
                     /gene="rhaS"
                     /locus_tag="ACHUU3_001849"
     CDS             complement(14204..15040)
                     /gene="rhaS"
                     /locus_tag="ACHUU3_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR2081932.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            15328..16797
                     /gene="rhaB"
                     /locus_tag="ACHUU3_001850"
     CDS             15328..16797
                     /gene="rhaB"
                     /locus_tag="ACHUU3_001850"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR2081933.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            16794..18053
                     /gene="rhaA"
                     /locus_tag="ACHUU3_001851"
     CDS             16794..18053
                     /gene="rhaA"
                     /locus_tag="ACHUU3_001851"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR2081934.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            18295..19119
                     /gene="rhaD"
                     /locus_tag="ACHUU3_001852"
     CDS             18295..19119
                     /gene="rhaD"
                     /locus_tag="ACHUU3_001852"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR2081935.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            19129..19443
                     /gene="rhaM"
                     /locus_tag="ACHUU3_001853"
     CDS             19129..19443
                     /gene="rhaM"
                     /locus_tag="ACHUU3_001853"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR2081936.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            19744..20190
                     /locus_tag="ACHUU3_001854"
     CDS             19744..20190
                     /locus_tag="ACHUU3_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR2081937.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            20201..21652
                     /locus_tag="ACHUU3_001855"
     CDS             20201..21652
                     /locus_tag="ACHUU3_001855"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFR2081938.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            21642..22712
                     /locus_tag="ACHUU3_001856"
     CDS             21642..22712
                     /locus_tag="ACHUU3_001856"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR2081939.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            22712..24460
                     /locus_tag="ACHUU3_001857"
     CDS             22712..24460
                     /locus_tag="ACHUU3_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFR2081940.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(24510..>25549)
                     /locus_tag="ACHUU3_001858"
     CDS             complement(24510..>25549)
                     /locus_tag="ACHUU3_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFR2081941.1"
                     /translation="KRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPE
                     APVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFKQ
                     GPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTI
                     NEMDKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAELK
                     NIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKG
                     GMNFSFINSADQYQLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREY
                     NEMVDDYNSLR"
BASE COUNT         6255 a         6572 c         6401 g         6321 t
ORIGIN      
        1 tataaacttt atgtttagtt acatcaggaa cgttctccat tgttacgatt tctggagata
       61 cttcgcgaat taaacgttcg aactcataaa gaagaggcca ttttttatca tcagccttat
      121 cttttccttg agtatatttt gaaaaaggtt ggcaaggtgc gcatcctgcc aatactttta
      181 cagacgcatc accataaagc ctcagcagtt cctcttttgt tactttagcg atatcttgct
      241 caataaactt agatttattg tttctttcat agggaaacct acattccccc tcaaggtcaa
      301 tgcctgcaac gacatcaagc cccgcaagct gcaaaccatg agttaatccg ccagcaccac
      361 aaaaaaggtc tatcaccttt acagtcatca ctcactccaa atctatacga tactcagtat
      421 acatcccttt ctacaaaatc tcgaggccta acttataaac tcatatttat tgagttaacg
      481 cttcactgag tttgactcta atactgttga gaatcccgac cactcatcag cgagcgggta
      541 agtgaatttt ttcccgtcat aatttacggt tgccccacgc gccagcacct caagctccca
      601 tcgctgcggc ctgataccgt tctgagcaag gtcaacgcgg atacgggtaa tttgcattcg
      661 ttccgaccgg gtcagtctgg ccgatggcgc tatttcatgc ggttttaacg ggcttccgtt
      721 tctttgctga cggtttggtc ttctcagtcc gtgttttaat gcacctctga gcgccctcac
      781 aacctccggg tcattccatt cgataacacc gtcatctacc agattaagca ctgctgcggc
      841 gtgctcagaa ggtgtgggag ccggtaacga agtatcacca ccagtgagct ttccacagtt
      901 attgacagga ctccgaggcg cggcgatgcc gctttttaaa gtcaaaggct caacgaccgg
      961 cactttcgga acaatgcgcc agtccgtcgt tctggtgata tgaatatgac gcgcgccgag
     1021 atgcggcgcg taaatgccga ccactctctc gacttcttcc tcgtactcgt taacttcatc
     1081 cgacggacta cgggcgaccc tgacagtctg acaatcgcgc gggacatttg ccccaccctg
     1141 cgcgctgata tacagcgcaa aatcaccact gtctgcggcg gcgcgtgcag cctcgacgcg
     1201 ctcgtcaaac tcatcagcaa tgctgacgcc gcgaggcaat ttgcgtagtt cacggtaagc
     1261 ccccattgtc ggcaggccaa ccgttttaaa ttgcggaatg cgccacgttg acgcccatgc
     1321 ggtaacagcc gcggcagtat ctttcagcgg tctgccgcta tcgttatcga gctgcccatc
     1381 cagtgcatag ccgtcgatat tttttgagat gtatttcgcg atataccccg cagcaccgcc
     1441 ccgattaaga tgttttgcct gaaaacggtt tcgcgcagct cctctttcgt cgccatcctc
     1501 tttgagcgcg tagcgacgca tgatttcgat aatctggtta cgctggcgtg gattacaaaa
     1561 aagcatcata tgccagtgcg gcgttccgtc gtggtgtggc tcgacgactc gcaaaccgta
     1621 gacctgtaaa tcattatcct tgaatgccgt gcgcatcagg ctccagatac ggcagagata
     1681 acgctgcgca tcctttggat taaatgcctc atcgttccag ccgtgattta gctggacggt
     1741 tttactttcg ccttttccaa cctgacgtgt cgggtgatac tttgacggcg cggtcagcgt
     1801 gataaacatc cccacatcac cttctgcggc ggcgtaacgc tcaataccgg caatggtgtt
     1861 catcagctcc atccggcgaa tttcaggatt agaaatactg cccatcacct tactgataag
     1921 gtcgatgcgc tcgcctgttt ccctgttttc gagatcacac gatttaagaa attccagatt
     1981 tgcctggcgg cgtgcacgca catcacgaat ggcatgttta ctggcataag gagaacggtc
     2041 tttattgacc tccccgacag caatcagtaa cgcctcatgc cagcgcatac gctggccttt
     2101 aagctgacta atccaccact catcgttaaa cagacgggca atggcagaat atgcctgcct
     2161 cgtggtcatc tgtcctttac ggtatttttt ccagtagaga ggggaaatat tgaaagcacg
     2221 tgcagcgcca gcaacatgac catagaggtg agcctgcgcc tcatccgtaa acagcgattc
     2281 tttttcgcca tgcgcatcca cccaggcatc gctgagttcc tcatacatca tgaaaagctg
     2341 cgatgagata cgggcggcaa actttttcag ctccttgtca ttcattcccg gcaggcgcgc
     2401 atagtggtca cgctcagcca gaaacagcat cgacgcgtcg gtgttcattt catggcgctg
     2461 attcacacgc tcaatgcgcg gccataaacg acgctgaaaa gtggatgtga ggaaataaaa
     2521 cccgtgcacc gggcttttat tgcgccggat gtagtcatag cgtgacgtaa acagcgagcg
     2581 caaaaagtaa ggcaggcggt taatcgtgga taaaacacct tgcacctgac gcatctcgtc
     2641 acgtgtaagg ggtctttcgc gcccgacagc ctcgcgtggc gcgttccatg cataagcacc
     2701 ggtaaacgcc ttaccggtgc ctgcagcaaa tgctgacgga gggacaaaac gcccggaggc
     2761 tttaacggcc atatgagcca aaagcctctg aacaacgctt gctgagttgc tcaacctgcg
     2821 cgtttaaatc agcaaaagac tttgcgcttc cggtcagaat atcgtgatgc atcaggccgg
     2881 aaacgagctg gcttaatttc gggtaataac caaccaccgc cagccattcc tgaccggcgt
     2941 ttttaccgct ttccgctctc tttttctcgt ggagaataaa ctggaagctg tcactggtaa
     3001 cgacataacg ttcgccaatt tcaatacgaa tactcatgcc gttctccggt aatgtttgtt
     3061 ttttgcttca aagactgact gacaggaaac acaacgcgtg gctgacggat aagccgcacg
     3121 acgggcagca ggaattggcg cgtcacactc ttcgcaaacc agcgcagaag caccgcaatg
     3181 ttttaccctt gccgcgttaa tctggcgctc cagtaattca gcctgttgtt cctgaataaa
     3241 atctatgttg tccggcatta tcagctcctt ttatcgttaa gtttcctgga tacatcagcg
     3301 caataactgg caagttctgt cgttaatttt gtcagttcat ccactgagga aatttgcttg
     3361 tggaatacag cgcgtttaac aagtaaattg accacatcag acaggaggtt taattcattc
     3421 tgataaatcg cgataacaga ttcagtgatg tcgcgttttt ctttatcaag acaaagttga
     3481 ataagagaca agtcgccatt tttcataacg gcgattttta aggcgttatt cagtaataca
     3541 actgaatgag aacaggacat caaagcacct ccccgcgaga caatccgata ttgtgaaatt
     3601 tttccgactc ctgactgagc agctcgacta tctccacgcg ggataactcc gcctttgtga
     3661 tatggcgaat catggcgtca agatgagaag aaaagcgcgt cgcagcgtcg gcctgtgctt
     3721 cggttctggc ctgttgcagc agtaatgcgt atttaccgca ctgattttca gaaactgtat
     3781 gcatgacttt ctccaggcaa aaagaagccc cgcacaatta agtgcgttaa aaactctggt
     3841 taattattta atgcaaatat tgctctggtt ttaccgacgt cagaattgtc ggtgcatact
     3901 caaacagact gaatagctca cgtaatgcac ggaataaagc atcacgccag taacatgact
     3961 cttcattaat tcgccagtat ggctgattga attctttttc agtcaatccg gcatgcataa
     4021 ataaagtacg acgctgactg actgttaaaa aactaatata tgcatactca cttgcgccaa
     4081 cctgacggcg ttttgagaat gccccacgca attcatcaat tgcacaaacc agccgttcac
     4141 gttcgacgtc gttcatttct tcaaaacgca tcgttgcgtg acgttgtttt aactgcgcat
     4201 gaaagcaaac tgttagccgt tcgcgctcca tcatctgatt ataataatca catgtatcct
     4261 gccaacgagg gacggcaaga tgcttaccaa ttatccggcg catagctgct ggctgttttt
     4321 cgacgagatt gagcgtcatc actgtcattt ccagaccctc cggcttttca gaaaggtcag
     4381 agcctttttt aacggactct gttttttggt gcggataatg attcccttgc gacccttccc
     4441 gtgggtgatg gtgaagtcaa tcgccctagg gctttcgtta cgcaataact gagcaataca
     4501 acgcggctca ttcataatca caaccccatc cacaaaagcc atgcatcacg ctgttcaacc
     4561 ggtcggttat aaaacgcctc acgtacagcg cgattaaact caggaatgaa aacccatttt
     4621 tcaccggcac gagccttcgg tttgcaagga tcacgcaatt caataattgg taatttattt
     4681 gccttcacca tttcactgac ggctgtcttt ggcttcccta ataaatcagc aaatttatcc
     4741 acatgaaccg catcaagcgg atactgaatc acataatttt cagcgtccat atatggtacc
     4801 ctcataggat ccagcccttt ctaaaccact caaaaccgtt tagacgctgg tttattctca
     4861 aatcaatgga acctatatag gttccagttt tgagggaatt tagtccctat ataggcacca
     4921 tgtcaaatga aattaagcga aaagattaag gccttgcgtg aggctgaagg gctaagccaa
     4981 tcaaaattct gtgaaatcat agagttaccg ctaagcacac ttaaaaaata tgaaggagga
     5041 aactttgaac ccggtggcac agctttgcta aaaatcacta tgcatcccac attccaaaaa
     5101 tatgctctat ggcttatgac agataaaacc gcgccggacg caggacaaat cgcaccggct
     5161 ctcgcgcaca ttgggccaga gtcaacagag tccaaccact ccgcgaaaag gattggctaa
     5221 ctctatataa agattacatt ttcaccattt gctaccaaga tggtgaatac agcgccggag
     5281 ggctttctta tggcaattaa gaagctcgat gatggtcgct atgaagtgga cattagacct
     5341 cgcggtcgcg acggaaaacg catccgcagg aaattcgaaa gaaaagctga agcactagca
     5401 tttgagcgat acacaatcgc caatgccagt cagaaagaat ggggaggcca gcgagcagac
     5461 cgccggactt tgagtgagtt gctggacatc tggtggaaat atcacgggca aaaccacgag
     5521 catggaacaa aagagtttaa tcatctactc aaaaccatca gcggcatagg tgatatacca
     5581 gtgagcagga tgagcaaaag ggctttgatg gattatcgtt ccatgcgact acgtgatggt
     5641 atcagtgccg caacgataaa ccgtgacatg tacagattat ccggcatgtt cacaaaatta
     5701 attcaattgg atgaattttc cgggcaacac ccaattcacg gactgccgcc actggcggag
     5761 gccaaccctg aaatgacgtt cctggaaaaa gcagaaatcg aaaaactgtt aaatgttttg
     5821 gctggtgatg acttacttgt cgcgctttta tgtctgagca ctggaggaag atggacgaaa
     5881 gttgccacgc taaaaccagc acagattaca aattgcaggg ttaccttcct gaaaaccaaa
     5941 aacggtaaaa agcgaaccgt gccgatttct gaggaactgg agaaaaaagt taaagaggag
     6001 gccagcgcca aattattcaa agttgattat gagaaatttt gcgggatttt acgcagagtg
     6061 aaacctgata taccacccaa tcaggcaacc cacatcctgc ggcatacatt cgcaagccat
     6121 ttcatgatga atgggggcaa tataatcgca ctgcaacaga ttctgggaca tgcgagcatt
     6181 cagcagacga tggcctatgc gcaccttgcg cctgactatc tgcaaaatgc cgtcgctctg
     6241 aatccactaa aaggcggagt gacgttataa atttccccct ctgagtgtcc acatagtgtc
     6301 cacactcaag aaattttgaa accgctccaa acccttacag ctttcgataa gtaactgttt
     6361 ttacttttga ttgcatgtaa gtatttgata aaaaaaaccc cacatcatgt gggggaagac
     6421 agggatggtg tctatggcaa ggaaaacagg gtttactact gggaacgtga gttgctacta
     6481 ctcaatagct tcaacgatga acttttttgc cattgcgtca cgtcacgcaa ctgctccatt
     6541 cgttgttgat gtttctcgtt taaaaccgct tgctgctccg gcgttaacag gcgatacatt
     6601 tggttgcgga cttttgccat ctcaacctga cgagcaattt gctcattcgc cattttttct
     6661 gcctgtgcgc gcacagcgtt ttcatcaaaa ttttctgcgg tgacaaggcg atgcattgtc
     6721 tccagttcgc taacattaac aggaggctgt tcgtgccggg cctgttgcat aagatctcgc
     6781 atctgctgac gctgatgttc ggttaaactt atgccgtcga acatatggct ctgcgtactg
     6841 cgctgcgtaa gttcttcacc cggatgccag ttatcgcctg aaccgacttc agcagcgtgg
     6901 cttaatgaac tgactgccag cgttgaggcc atgacggcag cggtaactat gcgcatcatt
     6961 tgctcccaaa atctttctgt cgcgattcaa cgatagagag tttacgattc aggctgcaaa
     7021 catgcgtcag ggggtgtaaa acaacgtaaa gtcatggatt agcgacgtct gatgacgtaa
     7081 tttctgcctc ggaggtattt aaacaatgaa taaaatcctg ttagttgatg atgaccgaga
     7141 gctgacttcc ctattaaagg agctgctcga gatggaaggc ttcaacgtga ttgttgccca
     7201 cgatggggaa caggcgcttg atcttctgga cgacagcatt gatttacttt tgcttgacgt
     7261 aatgatgccg aagaaaaatg gtatcgacac attaaaagca cttcgccaga cacaccagac
     7321 gcctgtcatt atgttgacgg cgcgcggcag tgaacttgat cgcgttctcg gccttgagct
     7381 gggcgcagat gactatctcc cgaaaccgtt taatgatcgt gagctggtgg cacgtattcg
     7441 cgcgatcctg cgccgttcgc actggagcga gcaacagcaa aacaacgaca acggttcacc
     7501 gacactggaa gttgatgcct tagtgctgaa tccaggccgt caggaagcca gcttcgacgg
     7561 gcaaacgctg gagttaaccg gtactgagtt taccctgctc tatttgctgg cacagcatct
     7621 gggtcaggtg gtttcccgtg aacatttaag ccaggaagtg ttgggcaaac gcctgacgcc
     7681 tttcgaccgc gctattgata tgcacatttc caacctgcgt cgtaaactgc cggatcgtaa
     7741 agatggtcac ccgtggttta aaaccttgcg tggtcgcggc tatctgatgg tttctgcttc
     7801 atgataggca gcttaaccgc gcgcatcttc gccatcttct ggctgacgct ggcgctggtg
     7861 ttgatgttgg ttttgatgtt acccaagctc gattcacgcc agatgaccga gcttctggat
     7921 agcgaacagc gtcaggggct gatgattgag cagcatgtcg aagcggagct ggcgaacgat
     7981 ccgcccaacg atttaatgtg gtggcggcgt ctgttccggg cgattgataa gtgggcaccg
     8041 ccaggacagc gtttgttatt ggtgaccacc gaaggccgcg tgatcggcgc tgaacgcagc
     8101 gaaatgcaga tcattcgtaa ctttattggt caggccgata acgccgatca tccgcagaag
     8161 aaaaagtatg gccgcgtgga actggtcggt ccgttctccg tgcgtgatgg cgaagataat
     8221 taccaacttt atctgattcg tccggccagc agttctcaat ccgatttcat taacttactg
     8281 tttgaccgcc cgctattact gctgattgtc accatgttgg tcagtacgcc gctgctgttg
     8341 tggttggcct ggagtctggc aaaaccggcg cgtaagctga aaaacgctgc cgatgaagtt
     8401 gcccagggaa acttacgcca gcacccggaa ctggaagcgg ggccacagga attccttgcc
     8461 gcaggtgcca gttttaacca gatggtcacc gcgctggagc gcatgatgac ctctcagcag
     8521 cgtctgcttt ctgatatctc tcacgagctg cgcaccccgc tgacgcgtct gcaactgggt
     8581 acggcgttac tgcgccgtcg tagcggtgaa agcaaggaac tcgagcgtat tgaaaccgaa
     8641 gcgcaacgtc tggacagcat gatcaacgat ctgttggtga tgtcacgtaa tcagcaaaaa
     8701 aacgcgctgg ttagcgaaac catcaaagcc aaccagttgt ggagtgaagt gctggataac
     8761 gcggcgttcg aagccgagca aatgggcaag tcgttgacag ttaacttccc gcctgggccg
     8821 tggccgctgt acggcaatcc gaacgccctg gaaagtgcgc tggaaaacat tgttcgtaat
     8881 gctctgcgtt attcccatac gaagattgaa gtgggctttg cggtagataa agacggtatc
     8941 accattacgg tggacgacga tggtcctggc gttagcccgg aagatcgcga acagattttc
     9001 cgtccgttct atcggaccga tgaagcacgc gatcgtgaat ctggcggtac aggattgggg
     9061 ctggcgattg ttgaaaccgc cattcagcag catcgtggct gggtgaaggc agaagacagc
     9121 ccgctgggcg gtttacggct ggtgatttgg ttgccgctgt ataagcggag ttaaactccg
     9181 catttgtagg caggataagg cgtttacgcc gcatccggca tttgagcagg atgcctgatg
     9241 cgacgctgat agcgtcttat caggcctaca ctccgggggt tattttcccc acaaccggcg
     9301 ggaaaaatct tcgatcttgc cgctcagtcg gcgcttttgc atcgtccgcg tccagctttt
     9361 cgataacccg gcggcggaga gtaaacggtg atactgatcg tcatcaaacg gcatatgcca
     9421 tgcgatggcg gcagcctcct gcacggtcac atcgctgaca cgggaaacca actccagcgg
     9481 cgcgtccgca gacactttcc ctggcgctat cacgctatac aaccacccca ccttacctgt
     9541 gttttgcatc aactgcgcta tatcgctgat atcaaaatgg taattgagtt tatagcaagg
     9601 cgagcgcggc tggctgacct gaattaatgc ctctccccag cggaaaatat cgcccatatg
     9661 aacattactt tccgtgagac cgtcggttga gagattttcg ccaaacgcag gcgcaacaaa
     9721 caactccgcc tgttccggaa attcccgcac ccagtagaga tagtgctcac gaggataatg
     9781 acacagcgct ctgtctggcc cgccgtgaac ctttttctcc gcctgctcgt caccttccag
     9841 ccccagctcc gtcagcatca actcaccatc aacctggatt ttggcaattg cgctgggttt
     9901 gccttcggga taagcctgaa tcttgcctgt gtatacatca accggatatc gcatctgttc
     9961 cctctccacg agaaatttcc ccattagacc atatttattt tatgtcaggc gtgctgtttt
    10021 cgcggtaagt atccaggcag gaactcaata gcaaaccgga cgaacgttag ttcatccggc
    10081 tgtggggatt aaggaagcag gtttatttca ccgtacctaa aatttcgatt ttcgctgctc
    10141 tggctttgac tttacgtgac cagatagagg taagaattgg caccagaatc gaagtcacaa
    10201 tgaccgccgt cgctaccagc gaagttgctg ccggagccat cggtttaaac gcaggcacca
    10261 tttctgcaat cagcacaggt gtcgctaccg cggcccctgc ggaactggaa gcggcaattc
    10321 cggcagtgcc atcgccaccg ccaatcaatt tatcggcgat aatcaacgga ataccggtca
    10381 cgataattac tgccacaccc aacaggatcc ccagcaaacc agtctgggca atcacagtca
    10441 aatcaatggt attgcccagc gcgaaggcaa agaatggaat cagcgtttgc actgctttgc
    10501 tgaaaaattc tcgtaattca gggtcaagat tccccagggc aaagcccacc aggaacggta
    10561 atactgcgcc gacgaaaaca tgcggttcaa acgaggcaat cccggcagtg cccagaataa
    10621 tcatcgtcat gagcggcccg gactccaacg acatcaacac aaatgcccca gcttcttctt
    10681 tcgtaccgta ctgctgcatg atggaagcgt aaagtccgcc gttggtcata tccatcgccg
    10741 ccaccagcgc cagcgttgaa agtccggcaa agaatccaac ttcaacacca tgttccggaa
    10801 taatgcgcga ggcaattgcc gcaaccaccc acgcgacggc aattttagtt accaccagcg
    10861 taccggattt acgcagtacc gttcccgtcg cgcttaattt tattgacgcc cccatgcaaa
    10921 aaaaccacac cgccagaatg ggcaccgtac cggtaatcat cccgttggtg aatgatccaa
    10981 aatatttccc cgcccccggc gagaaggtgt ggcacagtgc gccaaggaat agcgggacga
    11041 gcatcatccc ccccgggatt ttctcaatcg agcgttttat ctgcatttcc atcacctgtc
    11101 ggcatatttt taagggctga tgcgtaattc ctcaatggag catcagtgtt gatggaaacg
    11161 aagatagaca ttcatttaat caataaaagt gatcaaaacc acatcaattg aaacgctgtt
    11221 ttatttttat cggatcattg tttttatttt gaaattgatc acaaaaaaac caccgccgtt
    11281 ggcgatggtt cattttttaa gctgatatgc ggcctatacg cctcattgca gcaggcggca
    11341 aatgattatt ttttcgccgc aaaacgtgcc gctgcttcgt cccagttcac cacgttccag
    11401 aactctttaa tgtagtccgg acggcggttc tggaatttca ggtagtaagc atgttcccac
    11461 acatccaggc ccagaatcgg gaagccggaa gcgccagaaa tagcttcacc catcagcgga
    11521 gaatcctggt tagcagtaga aaccaccgcc agtttatcgc ctttcagcac cagccatgcc
    11581 cagccggaac caaagcggga agctgccgct ttttcaaatt ctgctttgaa gttatcaacg
    11641 gagccgaagt cacgttcgat agccgctttc aggtcaccct gcagggtggt gccttttttc
    11701 agacctttcc agaacaggct gtggttagcg tgaccgccag cgttgttgcg cagtacggtt
    11761 ttcttgtctg ctggcagctg gtccagtttg gtgatcagct cttcaaccgg caggttggca
    11821 aattctggca ggctttccag cgccgcgttg gcgttgttta cgtaggtctg atggtgtttg
    11881 gtgtggtgga tttccatggt ctgcttatcg aagtgcggtt ccagggcatc gtaagcatac
    11941 ggcagggatg gcagggtata gctcatattc atctccagta ttgtcgggcg gccgattgtt
    12001 aatgccgcgt aagcagttgg ttcattatag ttaattaaat gatattgaaa atgattatca
    12061 atgccgtact tttcgtaagg gtatggtttt gcaggaaaat gcccgagatg tgaagcaaat
    12121 cacccactta atgccgtgat tgccagtaaa tcgacaacgg cggcaacagg cgaaaggtta
    12181 atcgacagca cgatttttac actcatctcg tcggagatgt gaagcgacga aaaatgatga
    12241 ggataagaag atgagtaacg cgattacgat ggggatattt tggcatttga tcggcgcggc
    12301 cagtgcagcc tgtttttacg ctccgttcaa aaaagtaaaa aaatggtcat gggaaaccat
    12361 gtggtcagtc ggtgggattg tttcgtggat tattctgccg tggaccatca gcgccctgtt
    12421 actaccggat ttctgggcgt attacagctc gtttagtctc tctacgctgc tgcctgtttt
    12481 tctgttcggc gctatgtggg ggatcggtaa tatcaactat ggcctgacca tgcgttatct
    12541 cggcatgtcg atgggaattg gcatcgccat tggcattacg ttgattgtcg gtacgctgat
    12601 gacgccaatt atcaacggca atttcgatgt gctgattaac accgaaggcg gacgcatgac
    12661 gttgctcggc gttctggtgg cgctgattgg cgtagggatt gtaactcgag ccgggcagtt
    12721 gaaagagcgc aagatgggca ttaaagccga agagttcaat ctgaaaaaag ggctggtgct
    12781 ggcggtgatg tgcggcattt tctctgccgg gatgtccttt gcgatgaacg ccgcaaaacc
    12841 gatgcatgaa gccgctgccg cacttggcgt cgatccactg tatgtcgctc tgccaagcta
    12901 tgttgtcatc atgggcggcg gcgcgatcat taacctcggt ttctgtttta ttcgtctggc
    12961 aaaagtgaag gatttgtcgc taaaagccga cttctcgctg gcaaaaccgc tgatcattca
    13021 caatgtgtta ctctcggcac tgggcgggtt gatgtggtat ctgcaattct ttttctatgc
    13081 ctggggccac gcccgcattc cggcgcagta tgactacatc agttggatgc tgcatatgag
    13141 tttctatgta ttgtgcggcg gtatcgtcgg gctggtgctg aaagagtgga acaatacagg
    13201 acgccgtccg gtaacggtgt tgagcctcgg ttgtgtggtg attattgtcg ccgctaacat
    13261 cgtcggcatc ggcatggcga attaatcttt ctgcgaattg agatgacgcc actggctggg
    13321 cgtcatcccg gtttcccggg taaacaccac cgaaaaatag ttactatctt caaagccaca
    13381 ttcggtcgaa atatcactga ttaacaggcg gctatgctgg agaagatatt gcgcatgaca
    13441 cactctgacc tgtcgcagat attgattgat ggtcattcca gtctgctggc gaaattgctg
    13501 acgcaaaacg cgctcactgc acgatgcctc atcacaaaat ttatccagcg caaagggact
    13561 tttcaggcta gccgccaacc gggtaatcag cttatccagc aacgtttcgc tggatgttgg
    13621 cggcaacgaa tcactggtgt aacgatggcg attcagcaac atcaccaact gcccgaacag
    13681 caactcagcc atttcgttag caaacgacac atgctgacta ctttcatgct caagctgacc
    13741 gataacctgc cgcgcctgcg ccatccccac gctacctaag cgccagtgtg gttgccctgc
    13801 gctggcgcta aatcccggaa tcgctccctg ccagtcaaga ttcagcttca gccgctccgg
    13861 gcaataaata atattctgca aaaccagatc gttaacggaa gcgtaggagt gtttatcgtc
    13921 agcatgaata taaaagagat cgccacgggt aatgcgataa ggacgatcgt tgagtacatg
    13981 cagaccatta ccgcgccaga caatcaccag ctcacaaaaa tcatgtgtat gttcggcaaa
    14041 gacatcttgc ggataacggt cagccacagc gactgcctgc tggtcgctgg caaaaaaatc
    14101 atctttgaga agttttaact gatgcgccac cgtggctacc tcggccagag aacgaagttg
    14161 attattcgca atatggcgta caaatacgtt gagaagattc gcgttattgc agaaagccat
    14221 cccgtccctg gcgaatatca cgcggtgacc agttaaactc tcggcgaaaa agcgtcgaaa
    14281 agtggttact gtcgctgaat ccacagcgat aggcgatgtc agtaacgctg gcctcgctgt
    14341 ggcgtagcag atgtcgggct ttcatcagtc gcaggcggtt caggtatcgc tgaggcgtca
    14401 gtcccgtttg ctgcttaagc tgccgatgta gcgtacgcag tgaaagagaa aattgatccg
    14461 ccacggcatc ccaattcacc tcatcggcaa aatggtcctc cagccaggcc agaagcaagt
    14521 tgagacgtga tgcgctgttt tccaggttct cctgcaaact gcttttacgc agcaagagca
    14581 gtaattgcat aaacaagatc tcgcgactgg cggtcgaggg taaatcattt tccccttcct
    14641 gctgttccat ctgtgcaacc agctgtcgca cctgctgcaa tacgctgtgg ttaacgcgcc
    14701 agtgagacgg atactgccca tccagctctt gtggcagcaa ctgattcagc ccggcgagaa
    14761 actgaaatcg atccggcgag cgatacagca cattggtcag acacagatta tcggtatgtt
    14821 catacagatg ccgatcatga gcgcgtacga aacagaccgt gccaccggtg atggtatagg
    14881 gctgcccatt aaacacatga atacccgtgc catgttcgac aatcacaatt tcatgaaaat
    14941 catgatgatg ttcaggaaaa tccgcctgcg ggagccgggg ttctatcgcc acggacgcgt
    15001 taccagacgg aaaaaaatcc acactatgta atacggtcat actggcctcc tgatgtcgtc
    15061 aacacggcga aatagtaatc acgaggtcag gttcttacct taaattttcg acggaaaacc
    15121 acgtaaaaaa cgtcgatttt tcaagataca gcgtgaattt tcaggaaatg cggtgagcat
    15181 cacatcacca caatgaagca aattgtgaac atcatcacgt tcatctttcc ctggttgcca
    15241 atggcccatt ttcctgtcag taacgagaag gtcacgaatt caggcgcttt ttagactggt
    15301 cgtaatgaaa ttcagcagga tcacattatg acctttcgca attgtgtcgc cgtcgatctc
    15361 ggcgcatcca gtgggcgcgt gatgctggcg cgttacgagc gtgaatgccg cagcctgacg
    15421 ctgcgcgaaa tccatcgttt taacaatggc ctgcatagcc agaacggtta tgtcacctgg
    15481 aatgtggata gcctggaaag tgccattcgc cttggattaa acaaggtgtg cgaggaaggg
    15541 attcgtatcg atagcattgg gattgatacc tggggcgtgg actttgtgct gctcgaccaa
    15601 cagggtcagc gtgtgggcct gcccgttgct tatcgcgata gccgctccaa tggcctaatg
    15661 gcgcaggcac agcaacaact cggcaaacgc gatatttatc aacgtagcgg catccagttt
    15721 ctgcccttca atacgcttta tcaactgcgt gcgctgacgg agcaacaacc tgaacttatt
    15781 ccacacattg ctcacgctct gctgatgccg gattacttca gctatcgcct gaccggcaag
    15841 atgaactggg agtacaccaa cgccacgacc acgcaactgg tcaatatcaa tagcgacgac
    15901 tgggacgagt cgctactggc gtggagcggg gctaacaaag cctggtttgg tcgcccgacg
    15961 catccgggta atgtcattgg gcactggatt tgcccgcagg gtaatgagat tccggtggtc
    16021 gccgttgcca gccatgatac cgccagcgcg gttatcgcct cgccgttaaa cggttcacgc
    16081 gctgcttatc tctcttctgg cacctggtca ttgatgggct tcgaaagcca gacgccattt
    16141 accaatgaca cggcgctggc agccaacata accaatgaag gcggggcgga aggtcgctat
    16201 cgggtgctga aaaatattat gggcttatgg ctgcttcagc gagtgcttca ggagcggcaa
    16261 atcaacgatc tcccggcgct tatcgccgcg acacaggcac ttccggcctg ccgcttcatc
    16321 atcaatccca atgacgatcg ctttattaat cctgaggcga tgtgcagcga aattcaggct
    16381 gcgtgtcggg aaatggcgca accgctcccg gaaagtgatg ctgaactggc gcgctgcatt
    16441 ttcgacagtc tggcgctgct gtatgccgat gtgttgcatg agctggcgca gctacgcggt
    16501 gaagatttct cgcaactgca tattgtcggc ggcggctgcc agaacacgct gctcaaccag
    16561 ctatgtgccg atgcctgcgg tattcgggtg atcgccgggc ctgttgaagc ctcgacgctc
    16621 ggcaatatcg gcatccagtt aatgacgctg gatgaactca acaatgtgga tgatttccgt
    16681 caggtcgtca gcaccaccgc gaatctgacc acctttaccc ctaatcctga cagtgaaatt
    16741 gcccactatg tggcgcagct tcactcgaca cgacagacaa aggagctttg cgcatgacca
    16801 ctcaactgga acaggcctgg gagctggcga aacagcgttt cgcggcggtg gggattgatg
    16861 tcgaggaggc gctgcgccaa cttgatcgtt tacccgtttc aatgcactgc tggcagggcg
    16921 atgatgtttc cggttttgaa aacccggaag gttcgctgac cggtgggatt caggctactg
    16981 gtaattatcc gggcaaagcg cgtaatgcca gtgagctacg agccgatctg gaacaggcta
    17041 tgcggctgat tccggggccg aaacggctta atttacatgc catctatctg gaatcagaca
    17101 cgccagtctc gcgcgaccag atcaaacctg agcacttcaa aaactgggtt gaatgggcga
    17161 aagccaatca gctcggtctg gattttaacc cctcctgttt ctcgcatccg ctaagcgccg
    17221 atggctttac gctctcgcat cccgacgaca gcattcgcca gttctggatt gatcactgca
    17281 aagccagccg tcgcgtttcg gcctattttg gcgagcaact cggcacacca tcggtgatga
    17341 acatctggat cccggatggt atgaaagata tcaccgttga ccgtctcgcc ccgcgtcagc
    17401 gtctgctggc agcactggat gaggtgatca gcgagaagct ggatccggcg caccatatcg
    17461 acgccgttga gagcaaattg tttggcattg gcgcagagag ctacacggtt ggctccaatg
    17521 agttttacat ggggtatgcc accagccgcc agactgcgct gtgcctggac gccgggcatt
    17581 tccacccgac tgaagtgatt tccgacaaga tttccgccgc catgctgtat gtaccgcagt
    17641 tgctgctgca cgtcagccgt ccggttcgct gggacagcga tcacgtggtg ctgctggatg
    17701 atgaaaccca ggcgattgcc agtgagattg ttcgtcacga tctgttcgac cgggtgcata
    17761 tcggcctcga cttctttgat gcctctatca accgcattgc cgcgtgggtc attggtacac
    17821 gcaatatgaa aaaagccctg ctgcgtgcgt tgctggaacc taccgctgag ctgcgcaagc
    17881 tggaagcggc gggcgattac actgcgcgtc tggcgctact ggaagaacag aaatcgttgc
    17941 cgtggcaggc ggtctgggaa atgtattgcc aacgtcacga tacgccagca ggtagcgaat
    18001 ggctggagag cgtgcgggct tatgagaaag cgattttgag ccagcgtggg taaacactgc
    18061 cggatgcggc gcgagcgcct tatccggcct acaggtcgaa tgaccatgcg tgctgccacc
    18121 ctcaccccgg ccctctccct gaaagggcga gggagaaaaa catagcctcg tgcggcaacg
    18181 gagctgaaca ccgctccgga cagtcccctc gcccctacgg ggagagggtt agggtgaggg
    18241 gaacaagccg caccatgtct aaacaaggca acctaattta caggaacaca gaacatgcaa
    18301 aacattaccc agtcctggtt tgtccaggga atgatcaaag ccaccaccga tgcctggctg
    18361 aaaggctggg acgagcgcaa tggtggtaac ctgacgctgc gcctggatga cgccgatatc
    18421 gcgccttatc acggcaactt tcatgcacaa ccgcgctata ttcccctcag ccaacccatg
    18481 cctttactgg caaatacgcc gtttattgtc accggctccg gcaaattctt ccgtaacgtc
    18541 cagcttgatc ctgcagctaa cttaggcgtc gtaaaagtcg acagcgacgg cgcgggctac
    18601 catattctct gggggttaac caacgaagcc gttccaactt ccgaacttcc ggctcacttc
    18661 ctttcccact gcgagcgcat taaagccacc aacggtaaag atcgggtgat catgcactgc
    18721 cacgccacca acctgatcgc cctcacctat gtacttgaaa acgacaccgc ggtctttact
    18781 cgccaactgt gggaaggcag caccgagtgt ctggtggtat tcccggatgg cgttggcatt
    18841 ctgccgtgga tggtgcccgg cacggacgaa atcggccagg cgaccgcaca agagatgcaa
    18901 aaacattcgc tggtgttgtg gcccttccac ggcgtcttcg gcagcgggtc gacgttggat
    18961 gaaacattcg gtttaatcga caccgcagaa aaatcagcac aagtattagt gaaggtttat
    19021 tcgatgggcg gcatgaaaca gaccatcagc cgtgaagagt tgatagcgct cggcaagcgc
    19081 ttcggcgtta caccactagc tagtgcgctg gcgctgtaag gagcaaacat gatccgcaaa
    19141 gcctttgtca tgcaggttaa ccccgacgcc cacgaagagt atcagcgtcg gcataacccc
    19201 atctggccgg aactggaagc agtgttgaaa tctcacggtg cgcataacta cgccatttac
    19261 ctcgacaaag cgcgtaatct gctgtttgcc acggtagaga ttgaatctga agaacggtgg
    19321 aatgcggtag ccagcactga tgtttgccaa cgttggtgga aatatatgac cgatgttatg
    19381 cccgctaacc cggataacag cccggtgagt agcgagctgc aagaagtgtt ttacctgccg
    19441 taattgccca atgtgccaga gatgcctccg gcacaatatt ttaaatttcg atttgaaaaa
    19501 ttaaacatat tacatctccc ggtaagttat atttccctga tacattgtga gtaaatcaca
    19561 aaaataatga ataacccatt aatgattcat gtggtttatt taaataacca ttatgtgcat
    19621 tactccgcaa atctgacctt tcactctgtc tcaaattgtc attgtttacc gacatatcgc
    19681 ctctcatcac ttaatcgctc caccattaca attgattaaa atatatttta tggagtccga
    19741 ttaatggcag ctcttactgc aagctgtatt gacctgaata ttcagggcaa tggcgcttat
    19801 tccgttctga agcagttggc gacaatagcg ttacaaaacg gttttatcac cgactcacac
    19861 cagttcctgc aaaccctgct gctgcgcgaa aaaatgcact ctactggatt tggttccggt
    19921 gtcgccgtgc cgcacggtaa aagcgcctgc gttaaacaac cgtttgtatt attcgcccgc
    19981 aaagcgcagg ctattgactg gaaagccagc gatggcgaag acgtcaattg ctggatctgc
    20041 ctcggcgtgc cgcaaagcgg cgaagaggat caggtcaaaa tcatcggcac actgtgtcgc
    20101 aaaattatcc accaggattt tattcatcaa ctgcaacagg gcgacgccga ccaggtgctt
    20161 gccttgttaa atcaaaccct cagctcataa ggaagtggcg atggagtcat ccttatgtat
    20221 tgtcgcgatc accaactgcc ccgccgggat cgctcacacc tacatggtgg cggaagccct
    20281 ggaacagaaa gcccgttctc tcggccatac cataaaagtg gaaactcaag ggtccagtgg
    20341 cgttgaaaac cgcttatcca gcgaagagat tgccgctgcc gattacgtca ttctcgctac
    20401 cgggcgtggc ctgagcggtg atgatcgcgc gcggtttgcc gggaagaaag tttatgagat
    20461 tgccatctcc caggcgttga aaaatatcga ccagattttc agcgaattac cgacaaactc
    20521 gcagcttttt gccgcagata gcggcgtgaa gcttggcaaa caggaagtgc aaagcggcag
    20581 cgtgatgagc cacctgatgg cgggcgtctc tgcggcgctg ccgtttgtca tcggcggtgg
    20641 tatcctggtg gcgcttgcca acatgctggt gcagttcggt ttgccgtata ccgatatgtc
    20701 gaaaggcgcg ccgtcgttta cctgggtagt tgaatccatc ggttaccttg gcttcacctt
    20761 tatgatcccc atcatgggcg cttatattgc ctcgtcgatt gccgataaac cggctttcgc
    20821 tccggcgttt ctggtttgct atctggcgaa cgacaaagcg ctgctcggca cccagtcggg
    20881 tgcgggattc ctcggcgcgg tggtgctggg gctggcgatt ggctatttcg tcttctggtt
    20941 ccgcaaagtg cgccttggca aagcattaca accgctgctc ggttcaatgc tgatcccgtt
    21001 cgttactctg ctggttttcg gcgtgctcac ttactacgtt atcgggccgg tgatgtccga
    21061 cctcatgggc gggctgctcc acttcctgaa taccattcct ccatcaatga agtttgcggc
    21121 agcgtttctg gtcggtgcga tgctggcgtt cgatatgggc ggccccatca ataaaaccgc
    21181 ctggttcttc tgcttctcac tgctggaaaa acacatttat gactggtacg ccatcgtcgg
    21241 cgttgtcgcg ttgatgccgc ccgttgcggc gggtcttgcg acgttcattg cgcccaaact
    21301 gtttacccga caggaaaaag aggctgccag tagtgccatt gtggtcggtg ccacagtcgc
    21361 aaccgaaccg gctatacctt acgctctggc agctccgttg ccgatgatta ccgccaatac
    21421 gctggcgggt ggcattaccg gcgtgctggt gatcgctttc ggaatcaaac gtctggcacc
    21481 tggtttgggt atctttgacc cgctgatcgg cctgatgtcg ccggtgggtt cgttttatct
    21541 ggtgctagcg attggcctcg cgctgaacat ttcattcatt atcgtcctga aaggattgtg
    21601 gttacgccgc aaagcgaaag ccgcgcaaca ggagcttgtc catgaacatt gagttactgc
    21661 aacagttgtg cgaagccagc gccgtcagcg gcgatgaaca ggaagttcgc gacattctga
    21721 taaacacgct ggaaccttgc gttaatgaga tcacctttga tggtctgggc agctttgttg
    21781 cccgtaaggg gaataaaggt ccaaaagttg ccgttgtcgg gcatatggat gaagtcggct
    21841 ttatggtcac ccacatcgac gagagcggtt ttctgcgctt taccaccatt ggcggctggt
    21901 ggaatcagtc aatgctcaac caccgggtaa ccatacgcac acacaaggga tttaaaatcc
    21961 ctggtgtgat tggttccgtc gcacctcatg cgttaacgga aaagcaaaag caacagccgc
    22021 tgtcatttga tgagatgttc attgatattg gcgcgaacag tcgcgatgag gtggaaaaac
    22081 gcggtgtggc gatgggcgat tttattagcc cggaagccaa ttttgcctgc tggggcgaag
    22141 ataaagtggt cggcaaggcg ctggataacc gcatcggctg cgcaatgatg gctgaactat
    22201 tgcagacggt gaataatccc gaaattacgc tgtatggcgt cggcagcgtg gaagaagaag
    22261 ttgggctacg cggggcacaa acctcggcgg aacacattaa accggatgtg gtgattgtgc
    22321 tggataccgc tgtcgcaggt gatgttccgg gcattgataa cattaaatac ccgctgaaac
    22381 tgggccaggg gccggggctg atgctgtttg acaagcgcta cttccccaac cagaaactgg
    22441 tggcggcgtt aaaaagctgt gccgcacata acgatttacc gctgcaattt tccaccatga
    22501 aaaccggtgc gacggatggc gggcgctaca acgtgatggg cggcgggcgt ccggttgttg
    22561 cgctgtgcct gccaacccgc tatctgcacg ctaacagcgg catgatttca aaagccgatt
    22621 acgatgctct gctcacgttg atacgggatt ttctgaccac cttaaccgcg gagaaagtca
    22681 acgcgtttag ccagttccgt caggtggatt aatgctaaac gaacgccagt taaagattgt
    22741 cgatctgctg gagcaacagc cgcgcacgcc tggtgagctg gcgcaacaga ctggcgtttc
    22801 aggcaggacc atcctgcgtg atattgacta tctcaacttt acccttaacg gcaaagcccg
    22861 cattttcgcc agtggcagtg cgggctatca gctggaaatc ttcgagcgcc gcagcttttt
    22921 tcagttgctg caaaagcacg ataacgacga tcggctgctg gcgctgttat tactgaatac
    22981 ctttactccc cgtgcgcaac tcgcctcggc gcttaatttg ccagaaacct gggtagcaga
    23041 gcgtctgccc cggttaaaac agcgttatga acgcacttgt tgcctggcca gtcgccccgg
    23101 tttgggccat ttcattgatg agacagaaga gaaacgcgtt atcttgctgg cgaacttgct
    23161 gcgcaaagat ccgtttttaa ttccgctggc gggcgtaaca cgagacaatt ttcagcattt
    23221 atccacggcc tgcgacaacc aacaccgctg gccgctcatg cagggtgatt atctctccag
    23281 cctgattctg gcgatttacg ccctgcgtaa tcaactgacc gatgagtggc cgcaatatcc
    23341 cggtaacgag ataaaacaaa tcgttgaaca gagcggcatg tttcttggtg ataacgctgt
    23401 aagaaccctg acaggtttga tagagaaaca gcatcagcaa gcgcaggtaa tttcagccga
    23461 tcatgtgctg gggttgctgc aaagggtgcc aggcatcgcg tcattgaata ttattgatac
    23521 gcagctggtt gagaatatta ccgggcattt attacgttgc cttgccgcac cggtgtggat
    23581 tgccgagcac cgccaaagca gcatgaataa cctgaaagcc gcctggcctg cggcctttga
    23641 tatgagtctg cactttatta cgctactgcg tgaacagctc gatattcccc ttttcgatag
    23701 cgatctgatc ggtttgtatt ttgcctgtgc gctggaacgg catcaaaacg aacgccagcc
    23761 gatcattttg ctctcggacc agaacgcgat tgccactatt aatcagctcg ccattgagcg
    23821 cgatgtttta cattgtcggg tgattattgc ccgtagctta agcgagctta ttgccattcg
    23881 cgaagagatt gagccgttat tgatcattaa caacagccat tatttactgg atgacgcggt
    23941 aaataattac atcaccgtaa aaaatatcat tacggctgcc gatatcgaac aaataaaaca
    24001 ctttctggcg acggcattta ttcgccaaca gccggaacgc tttttctctg cccccggaag
    24061 ttttcattat tcgaatgtac gcggtgaaag ctggcaacat attacccggc aaatttgtgc
    24121 gcaattagtg gcacaacacc atattaccgc cgatgaagcg caacggatca tcgcccgcga
    24181 aggcgaaggt gaaaacctga ttgttaatcg cctcgccatc ccacattgct ggagcgaaca
    24241 ggagcgacgt tttcgtggat tttttattac cctcgcccaa ccagttgagg tgaataacga
    24301 agtcattaac catgtcttga tcgcctgcgc cgccgccgat gcgcgtcatg agctaaaaat
    24361 atttagctat ctggcaagcg tattgtgtca gcatccggca gaagttattg ccgggttaac
    24421 aggatatgag gcatttatgg agttacttca caaggggtga actgtgtcgg atgcgccgca
    24481 tccgacactg catttatcat caaaaacgtt taacgcaggc tattatagtc atcaaccatt
    24541 tcgttgtact cgcgtaacgc tcgtggaaat gagtcttcga ccatccagct tgtgtttgca
    24601 tcctgaagat gctctttatc ccagtcagag tacgggactt tatctctgac gcggcgaaca
    24661 tattttttag tctcgagttg atactggtct gccgaattaa tgaacgagaa attcataccg
    24721 cctttatccg cttctttggc ctgcgctacc agcgtttcca gttcagagac tttcttcatt
    24781 gccgcatcaa catcaaactt ctcttgcgaa atcaggttat taatttgttt cgctgaaatc
    24841 atgactgcca gagagtagta gtggaatgtt ttcccttctc tttcttcaat atttttcagt
    24901 tccgcaagct ggcgcttatc attaatttcc tgaatcgccg catgataaga ttctgctaca
    24961 ggttcaaagg attccagatt ttttaagaat gtctggtgca gcgttttccc tttcgcaaac
    25021 gcatcgtctt tataattttc ctgggtgtaa tatttatcca tttcgttaat ggtattaccc
    25081 aacgccacgg cagcatcaat ataactcacc gccacgccat caataggttg cagcgcaggc
    25141 gttaacgcca cagcactttt tacgcctttt tcacactcag cgaggttgga ttcactaatg
    25201 ccgtagatgc cataaacagt actctcttta ccggtaggcc cctgtttaaa atctttcagc
    25261 cagtcagcat aacgcgccag actgttctgt acagggatct gtaacttgtt gtagcacttg
    25321 atatagacat ccattttttc gtcgaaaacc gcttgttcat cgaccaccgg ttgcgcaggt
    25381 gtttcaggtt tagcttctgc ttttgcaacg ggagcttcag gagctggtgc ggcaggttga
    25441 ctattggcag gcgggggcgt ttctgtttcg gcttttttat catcacaacc cgtcagaccg
    25501 agggccatga tggcgactat aatagcggaa gataataaat tccttttca
//
LOCUS       DAZPLI010000047        25283 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZPLI010000047 DAZPLI010000000
VERSION     DAZPLI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25283
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.047"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            16..267
                     /locus_tag="ACHUU3_001859"
                     /pseudo
     CDS             16..267
                     /locus_tag="ACHUU3_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="involved in type II protein secretion; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            505..816
                     /gene="rpsJ"
                     /locus_tag="ACHUU3_001860"
     CDS             505..816
                     /gene="rpsJ"
                     /locus_tag="ACHUU3_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR2081942.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            849..1478
                     /gene="rplC"
                     /locus_tag="ACHUU3_001861"
     CDS             849..1478
                     /gene="rplC"
                     /locus_tag="ACHUU3_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR2081943.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1489..2094
                     /gene="rplD"
                     /locus_tag="ACHUU3_001862"
     CDS             1489..2094
                     /gene="rplD"
                     /locus_tag="ACHUU3_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR2081944.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2091..2393
                     /gene="rplW"
                     /locus_tag="ACHUU3_001863"
     CDS             2091..2393
                     /gene="rplW"
                     /locus_tag="ACHUU3_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR2081945.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2411..3232
                     /gene="rplB"
                     /locus_tag="ACHUU3_001864"
     CDS             2411..3232
                     /gene="rplB"
                     /locus_tag="ACHUU3_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR2081946.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3249..3527
                     /gene="rpsS"
                     /locus_tag="ACHUU3_001865"
     CDS             3249..3527
                     /gene="rpsS"
                     /locus_tag="ACHUU3_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR2081947.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3542..3874
                     /gene="rplV"
                     /locus_tag="ACHUU3_001866"
     CDS             3542..3874
                     /gene="rplV"
                     /locus_tag="ACHUU3_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR2081948.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            3892..4593
                     /gene="rpsC"
                     /locus_tag="ACHUU3_001867"
     CDS             3892..4593
                     /gene="rpsC"
                     /locus_tag="ACHUU3_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR2081949.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4606..5016
                     /gene="rplP"
                     /locus_tag="ACHUU3_001868"
     CDS             4606..5016
                     /gene="rplP"
                     /locus_tag="ACHUU3_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR2081950.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5016..5207
                     /gene="rpmC"
                     /locus_tag="ACHUU3_001869"
     CDS             5016..5207
                     /gene="rpmC"
                     /locus_tag="ACHUU3_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR2081951.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5207..5461
                     /gene="rpsQ"
                     /locus_tag="ACHUU3_001870"
     CDS             5207..5461
                     /gene="rpsQ"
                     /locus_tag="ACHUU3_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR2081952.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5626..5997
                     /gene="rplN"
                     /locus_tag="ACHUU3_001871"
     CDS             5626..5997
                     /gene="rplN"
                     /locus_tag="ACHUU3_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR2081953.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6008..6322
                     /gene="rplX"
                     /locus_tag="ACHUU3_001872"
     CDS             6008..6322
                     /gene="rplX"
                     /locus_tag="ACHUU3_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR2081954.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6337..6876
                     /gene="rplE"
                     /locus_tag="ACHUU3_001873"
     CDS             6337..6876
                     /gene="rplE"
                     /locus_tag="ACHUU3_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR2081955.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            6891..7196
                     /gene="rpsN"
                     /locus_tag="ACHUU3_001874"
     CDS             6891..7196
                     /gene="rpsN"
                     /locus_tag="ACHUU3_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR2081956.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7230..7622
                     /gene="rpsH"
                     /locus_tag="ACHUU3_001875"
     CDS             7230..7622
                     /gene="rpsH"
                     /locus_tag="ACHUU3_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR2081957.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7635..8168
                     /gene="rplF"
                     /locus_tag="ACHUU3_001876"
     CDS             7635..8168
                     /gene="rplF"
                     /locus_tag="ACHUU3_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR2081958.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8178..8531
                     /gene="rplR"
                     /locus_tag="ACHUU3_001877"
     CDS             8178..8531
                     /gene="rplR"
                     /locus_tag="ACHUU3_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR2081959.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8546..9049
                     /gene="rpsE"
                     /locus_tag="ACHUU3_001878"
     CDS             8546..9049
                     /gene="rpsE"
                     /locus_tag="ACHUU3_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR2081960.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9053..9232
                     /gene="rpmD"
                     /locus_tag="ACHUU3_001879"
     CDS             9053..9232
                     /gene="rpmD"
                     /locus_tag="ACHUU3_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR2081961.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9236..9670
                     /gene="rplO"
                     /locus_tag="ACHUU3_001880"
     CDS             9236..9670
                     /gene="rplO"
                     /locus_tag="ACHUU3_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR2081962.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9678..11009
                     /gene="secY"
                     /locus_tag="ACHUU3_001881"
     CDS             9678..11009
                     /gene="secY"
                     /locus_tag="ACHUU3_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR2081963.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DATVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11041..11157
                     /gene="rpmJ"
                     /locus_tag="ACHUU3_001882"
     CDS             11041..11157
                     /gene="rpmJ"
                     /locus_tag="ACHUU3_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR2081964.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11304..11660
                     /gene="rpsM"
                     /locus_tag="ACHUU3_001883"
     CDS             11304..11660
                     /gene="rpsM"
                     /locus_tag="ACHUU3_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR2081965.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11677..12066
                     /gene="rpsK"
                     /locus_tag="ACHUU3_001884"
     CDS             11677..12066
                     /gene="rpsK"
                     /locus_tag="ACHUU3_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR2081966.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12100..12720
                     /gene="rpsD"
                     /locus_tag="ACHUU3_001885"
     CDS             12100..12720
                     /gene="rpsD"
                     /locus_tag="ACHUU3_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR2081967.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            12746..13735
                     /gene="rpoA"
                     /locus_tag="ACHUU3_001886"
     CDS             12746..13735
                     /gene="rpoA"
                     /locus_tag="ACHUU3_001886"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR2081968.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            13776..14159
                     /gene="rplQ"
                     /locus_tag="ACHUU3_001887"
     CDS             13776..14159
                     /gene="rplQ"
                     /locus_tag="ACHUU3_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR2081969.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14266..14634
                     /locus_tag="ACHUU3_001888"
     CDS             14266..14634
                     /locus_tag="ACHUU3_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFR2081970.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            14645..15070
                     /gene="zntR"
                     /locus_tag="ACHUU3_001889"
     CDS             14645..15070
                     /gene="zntR"
                     /locus_tag="ACHUU3_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFR2081971.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15126..15344
                     /gene="arfA"
                     /locus_tag="ACHUU3_001890"
     CDS             15126..15344
                     /gene="arfA"
                     /locus_tag="ACHUU3_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFR2081972.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15341..15751)
                     /gene="mscL"
                     /locus_tag="ACHUU3_001891"
     CDS             complement(15341..15751)
                     /gene="mscL"
                     /locus_tag="ACHUU3_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFR2081973.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(15881..17257)
                     /gene="trkA"
                     /locus_tag="ACHUU3_001892"
     CDS             complement(15881..17257)
                     /gene="trkA"
                     /locus_tag="ACHUU3_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR2081974.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17279..18568)
                     /gene="rsmB"
                     /locus_tag="ACHUU3_001893"
     CDS             complement(17279..18568)
                     /gene="rsmB"
                     /locus_tag="ACHUU3_001893"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR2081975.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQHQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18614..19561)
                     /gene="fmt"
                     /locus_tag="ACHUU3_001894"
     CDS             complement(18614..19561)
                     /gene="fmt"
                     /locus_tag="ACHUU3_001894"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR2081976.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19576..20085)
                     /gene="def"
                     /locus_tag="ACHUU3_001895"
     CDS             complement(19576..20085)
                     /gene="def"
                     /locus_tag="ACHUU3_001895"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR2081977.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20215..21339
                     /gene="dprA"
                     /locus_tag="ACHUU3_001896"
     CDS             20215..21339
                     /gene="dprA"
                     /locus_tag="ACHUU3_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR2081978.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21311..21784
                     /gene="smg"
                     /locus_tag="ACHUU3_001897"
     CDS             21311..21784
                     /gene="smg"
                     /locus_tag="ACHUU3_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR2081979.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            21813..22355
                     /locus_tag="ACHUU3_001898"
     CDS             21813..22355
                     /locus_tag="ACHUU3_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR2081980.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22360..22932
                     /gene="tsaC"
                     /locus_tag="ACHUU3_001899"
     CDS             22360..22932
                     /gene="tsaC"
                     /locus_tag="ACHUU3_001899"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR2081981.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            22937..23755
                     /gene="aroE"
                     /locus_tag="ACHUU3_001900"
     CDS             22937..23755
                     /gene="aroE"
                     /locus_tag="ACHUU3_001900"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR2081982.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            23752..24009
                     /locus_tag="ACHUU3_001901"
     CDS             23752..24009
                     /locus_tag="ACHUU3_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR2081983.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(23985..24539)
                     /locus_tag="ACHUU3_001902"
     CDS             complement(23985..24539)
                     /locus_tag="ACHUU3_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR2081984.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            25012..>25283
                     /locus_tag="ACHUU3_001903"
     rRNA            25012..>25283
                     /locus_tag="ACHUU3_001903"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6442 a         5993 c         6616 g         6232 t
ORIGIN      
        1 cttctttaat gggcgctttg gatagtaaaa gcagaagtca ctgtagaggc tgaaacgccc
       61 gcttttaaac aatcaacaca acactatttc ttcaaaaaac agccactccc cgtcgtcgaa
      121 tccgtcgagg aggaagatga cccagacgtc gcggttgaga atgctccctc ttcttctgaa
      181 gacgaagaaa atactgtaaa agaaagcgac gagaaagccg gtttaagaga gcgtgtcaaa
      241 aatgcgctga acgaacttga gagataaccc gaaggctgtt tactttacta gcaatacgct
      301 tgcgttcggt ggttaagtat gtataatgcg cgggcttgtc gtagttgaca gcaggttcaa
      361 tctgaacctc agttagccga atttggctac ctaacaatgc tcccaatcgg ggagctacgt
      421 aagaacggtt acactctccc atcaatcgta atgggtctga ggagtaatca ttttcgttta
      481 taaaataatt ggagctctgg tctcatgcag aaccaaagaa tccgtatccg cctgaaagcg
      541 tttgatcatc gtctgatcga tcaagcaacc gcggaaatcg tcgagactgc caagcgcact
      601 ggtgcgcagg tccgtggtcc gatcccgctg ccgacacgca aagagcgctt cactgttctg
      661 atctccccgc acgtcaacaa agacgcgcgc gatcagtacg aaatccgtac tcacttgcgt
      721 ctggttgaca tcgttgagcc aaccgagaaa accgttgatg ctctgatgcg tctggatctg
      781 gctgccggtg tagacgtgca gatcagcctg ggttaatcag gtcattgagc gattgagagg
      841 ttgaaacaat gattggttta gtcggtaaaa aagtgggtat gacccgtatc ttcacagaag
      901 acggcgtttc tatcccagta accgtaatcg aagttgaagc aaaccgcgtt actcaggtta
      961 aagacctggc taacgatggc taccgtgcta ttcaggtgac caccggtgct aaaaaagcta
     1021 accgtgtgac caagcctgaa gctggccact tcgctaaagc tggcgtagaa gctggccgtg
     1081 gtctgtggga attccgcctg gctgaaggcg aagagttcac tgtaggtcag agcattagcg
     1141 ttgaactgtt tgctgaggtt aaaaaagttg acgtaactgg cacctctaaa ggtaaaggtt
     1201 tcgcaggtac cgttaagcgc tggaacttcc gtacccagga cgctactcac ggtaactcct
     1261 tgtctcaccg cgttccgggt tctatcggtc agaaccagac tccgggcaaa gtgttcaaag
     1321 gcaagaaaat ggcaggtcag atgggtaacg aacgtgtaac cgttcagagc cttgacgtag
     1381 tacgcgttga cgctgagcgc aacctgctgc tggttaaagg tgctgtcccg ggtgcaaccg
     1441 gtagcgacct gatcgttaaa ccagctgtga aggcgtaagg agatagcaat ggaattagta
     1501 ttgaaagacg cgcagagcgc gctgactgtt tccgaaacta ccttcggtcg tgatttcaac
     1561 gaagcgctgg ttcaccaggt tgttgttgct tatgcagctg gtgctcgtca gggtactcgt
     1621 gctcagaaga ctcgtgctga agtaactggt tccggtaaaa aaccgtggcg ccagaaaggc
     1681 accggccgtg cgcgttctgg ttctatcaag agcccgatct ggcgttctgg tggcgtgacc
     1741 tttgctgctc gtccgcagga ccacagtcaa aaagttaaca agaagatgta ccgcggcgcg
     1801 ctgaaaagca tcctgtccga actggtacgt caggatcgtc tgatcgttgt cgagaagttc
     1861 tctgtagaag cgccgaaaac taagctgctg gcacagaaac tgaaagacat ggctctggaa
     1921 gatgtgctga tcatcaccgg tgagctggac gaaaacctgt tcctggctgc gcgcaacctg
     1981 cacaaggttg acgtacgcga tgcaactggt atcgacccgg ttagcctgat cgccttcgac
     2041 aaagtcgtaa tgactgctga tgctgttaag caagttgagg agatgctggc atgattcgtg
     2101 aagaacgtct gctgaaggtg ctgcgtgcac cgcacgtttc tgaaaaagcg tctactgcga
     2161 tggaaaaatc caacaccatc gtactcaaag ttgctaaaga cgcgaccaaa gcagaaatca
     2221 aagctgctgt gcagaaactg tttgaagtcg aagtcgaagt cgttaacacc ctggtagtta
     2281 aagggaaagt taaacgtcac ggacagcgta tcggtcgtcg tagcgactgg aaaaaagctt
     2341 acgtcaccct gaaagaaggc cagaatctgg acttcgttgg cggcgctgag taagtcggag
     2401 gagtaataca atggcagttg ttaaatgtaa accgacatct ccgggtcgtc gccacgtagt
     2461 taaagtggtt aaccctgagc tgcacaaggg caaacctttt gctccgttgc tggaaaaaaa
     2521 cagcaaatcc ggtggtcgta acaacaatgg ccgtatcacc actcgtcata tcggtggtgg
     2581 ccacaagcag gcttaccgta ttgttgactt caaacgcaac aaagacggta tcccggcagt
     2641 tgttgaacgt cttgagtacg atccgaaccg ttccgcgaac atcgcgctgg ttctgtacaa
     2701 agacggtgaa cgccgttaca tcctggcccc taaaggcctg aaagctggcg accagattca
     2761 gtctggcgtt gatgctgcaa tcaaaccagg taacaccctg ccgatgcgca acatcccggt
     2821 tggttctact gttcataacg tagaaatgaa accaggtaaa ggcggtcagc tggcacgttc
     2881 cgctggtact tacgttcaga tcgttgctcg tgatggtgct tatgtcaccc tgcgtctgcg
     2941 ttctggtgaa atgcgtaaag tagaagcaga ctgccgtgca actctgggcg aagttggcaa
     3001 tgctgagcat atgctgcgcg ttctgggtaa agcaggtgct gcacgctggc gtggtgttcg
     3061 tccgaccgtt cgcggtaccg cgatgaaccc ggtagaccac ccacatggtg gtggtgaagg
     3121 tcgtaacttt ggtaagcacc cggtaactcc gtggggcgtt cagaccaaag gtaagaagac
     3181 ccgcagcaac aagcgtactg ataaattcat cgtacgtcgc cgtagcaaat aattttagag
     3241 gataagccat gccacgttct ctcaagaaag gtccttttat tgacctgcac ttgctgaaga
     3301 aggtagagaa agcggtggaa agcggagaca agaagcccct gcgcacttgg tcccgtcgtt
     3361 caacgatctt tcctaacatg atcggtttga ccatcgctgt ccataatggt cgtcagcacg
     3421 ttccggtatt tgtgaccgac gaaatggtcg gtcacaaact gggtgaattc gcaccgactc
     3481 gtacttatcg cggccacgct gctgataaaa aagcgaagaa gaaataaggt aggaggaaga
     3541 gatggaaact atcgctaaac atcgccatgc tcgttcttct gctcagaagg ttcgccttgt
     3601 tgctgacctg attcgcggta agaaagtgtc gcaggctctg gatattttga cctacaccaa
     3661 caagaaagcg gctgtactgg tcaagaaagt tctggaatct gccattgcta acgctgaaca
     3721 caacgatggc gctgacattg acgatctgaa agttacgaaa attttcgtag acgaaggccc
     3781 gagcatgaag cgcattatgc cgcgtgcaaa aggtcgtgca gatcgcatcc tgaagcgcac
     3841 cagccacatc actgtggttg tgtccgatcg ctgagactct ggagactagc aatgggtcag
     3901 aaagtacatc ctaatggtat tcgcttgggt attgtaaagc catggaactc tacctggttt
     3961 gcgaacacca aagaattcgc tgacaacctg gacagcgatt ttaaagtacg tcagtacctg
     4021 actaaggaac tggctaaagc gtccgtatct cgtatcgtta tcgagcgtcc ggctaagagc
     4081 atccgtgtaa ccattcacac tgctcgcccg ggtatcgtta tcggtaaaaa aggtgaagac
     4141 gtagaaaaac tgcgtaaggt cgtagcggac atcgctggcg ttcctgcaca gatcaacatc
     4201 gccgaagttc gtaagcctga actggacgca aaactggttg ctgacagcat cacttctcag
     4261 ctggaacgtc gcgttatgtt ccgtcgtgct atgaagcgtg ctgtacagaa cgcaatgcgt
     4321 ctgggcgcta aaggtattaa agttgaagtt agcggccgtc tgggcggcgc ggaaatcgca
     4381 cgtaccgaat ggtaccgcga aggtcgcgta ccgctgcaca ctctgcgtgc tgacatcgac
     4441 tacaacacct ctgaagcgca caccacttac ggtgtaatcg gcgttaaagt gtggatcttc
     4501 aaaggcgaga tcctgggtgg tatggctgct gttgaacaac cggaaaaacc ggctgctcag
     4561 cctaaaaagc agcagcgtaa aggccgtaaa taaggagcgt cgctgatgtt acaaccaaag
     4621 cgtacaaaat tccgtaaaat gcacaaaggc cgtaaccgcg gtctggcgca gggtacggat
     4681 gttagcttcg gcagcttcgg tctgaaagct gttggccgtg gtcgtctgac tgcccgtcag
     4741 atcgaagcag cacgtcgtgc tatgacccgt gcagttaagc gtcaaggtaa gatctggatc
     4801 cgtgtgttcc cggacaaacc gatcactgaa aagccgctgg cagtgcgtat gggtaaaggt
     4861 aaaggtaacg tggagtattg ggttgccttg attcagccgg gtaaagtcct gtatgaaatg
     4921 gacggtgttc cggaagagct ggcccgtgaa gcattcaagc tggcagcagc gaaactgccg
     4981 attaaaacca cctttgtaac taagacggtg atgtaatgaa agcaaaagag ctgcgtgaga
     5041 agagcgttga agagctgaac accgagctgc tgaacctgct gcgtgagcag ttcaacctgc
     5101 gtatgcaggc tgcaagtggc cagctgcaac agtctcacct gttgaagcaa gtgcgtcgcg
     5161 atgtcgcacg cgttaagact ttactgaacg agaaggcggg tgcgtaatga ccgataaaat
     5221 ccgtactctg caaggtcgcg ttgttagcga caaaatggag aaatccattg ttgttgctat
     5281 cgaacgtttt gtgaaacacc cgatctacgg taaattcatc aagcgtacga ccaaactgca
     5341 cgtacatgac gagaacaacg aatgcggtat cggtgacgtg gttgaaatcc gcgaatgccg
     5401 tccgctgtcc aagactaaat cctggacgct ggttcgcgtt gtagagaaag cggttctgta
     5461 atacagtaca ctctctcgat acgaataaac ggctcagaaa tgagccgttt attttttcta
     5521 cccatatcct tgaagcggtg ttataatgcc gcgccctcga tatggggatt tttaacgacc
     5581 tgattttcgg gtctcagtag tagttgacat tagcggagca ctaaaatgat ccaagaacag
     5641 actatgctga acgtcgccga caactccggt gcacgtcgcg taatgtgtat caaggttctg
     5701 ggtggctcgc accgtcgcta cgcaggcgta ggcgacatca tcaagatcac catcaaagaa
     5761 gcaattccgc gtggtaaggt caaaaaaggt gatgtgctga aggcggtagt ggtgcgcacc
     5821 aagaagggtg ttcgtcgccc ggacggttct gtcattcgct tcgatggtaa tgcttgtgtt
     5881 cttctgaaca acaacagcga gcagcctatc ggtacgcgta tttttgggcc ggtaactcgt
     5941 gagcttcgta gtgagaagtt catgaaaatt atctctctgg caccagaagt actctaagga
     6001 gcgaatcatg gcagcgaaaa tccgtcgtga tgacgaagtt atcgtgttaa ccggtaaaga
     6061 taaaggtaaa cgcggtaaag ttaagaatgt cctgtcttcc ggcaaggtca ttgttgaagg
     6121 tatcaacctg gttaagaaac atcagaagcc ggttccggcc ctgaaccaac cgggtggcat
     6181 cgttgaaaaa gaagccgcta ttcaggtttc caacgtagca atcttcaatg cggcaaccgg
     6241 caaggctgac cgtgtaggct ttagattcga agacggcaaa aaagtccgtt tcttcaagtc
     6301 taacagcgaa actatcaagt aatttggagt agtacgatgg cgaaactgca tgattactac
     6361 aaagacgaag tagttaaaaa actcatgact gagtttaact acaattctgt catgcaagtc
     6421 cctcgggtcg agaagatcac cctgaacatg ggtgttggtg aagcgatcgc tgacaaaaaa
     6481 ctgctggata acgcagcagc agacctggca gcaatctccg gtcaaaaacc gctgatcacc
     6541 aaagcacgca aatctgttgc aggcttcaaa atccgtcagg gctatccgat cggctgtaaa
     6601 gtaactctgc gtggcgaacg catgtgggag ttctttgagc gcctgatcac tattgctgta
     6661 cctcgtattc gtgacttccg tggcctgtcc gctaagtctt tcgacggtcg tggtaactac
     6721 agcatgggtg tccgtgagca gatcatcttc ccagaaatcg actacgataa agtcgaccgc
     6781 gttcgtggtt tggatattac cattaccact actgcgaaat ctgacgaaga aggccgcgct
     6841 ctgctggctg cctttgactt cccgttccgc aagtaaggta gggttactaa atggctaagc
     6901 aatcaatgaa agcacgcgaa gtaaaacgcg tagctttagc tgataaatac ttcgcgaaac
     6961 gcgctgaact gaaagcgatc atctctgatg tgaacgcttc cgacgaagat cgttggaacg
     7021 ctgttctcaa gctgcagact ctgccgcgtg attccagccc gtctcgtcag cgtaaccgct
     7081 gccgtcaaac aggtcgcccg catggtttcc tgcggaagtt cgggttgagc cgtattaagg
     7141 tccgtgaagc cgctatgcgc ggtgaaatcc cgggtctgaa aaaggctagc tggtaattgt
     7201 caccaattga atcacgggag taaagacaga tgagcatgca agatccgatc gcggatatgc
     7261 tgacccgtat ccgtaacggt caggccgcga acaaagctgc ggtcaccatg ccttcctcca
     7321 agctgaaagt ggcaatcgcc aacgtgctga aggaagaagg ttttattgaa gattttaaag
     7381 ttgaaggcga caccaagcct gaactggaac ttactctgaa gtatttccag ggcaaagctg
     7441 ttgtagaaag cattcagcgt gtcagccgcc caggtctgcg catctataaa cgtaaagatg
     7501 agctgccgaa agttatggcg ggtctgggta tcgcagttgt ttctacctct aaaggtgtta
     7561 tgactgatcg tgcagcgcgc caggctggtc ttggtggcga aattatctgc tacgtagcct
     7621 aatcggagga aaaaatgtct cgtgttgcta aagcaccggt cgttgttcct gccggcgttg
     7681 acgtaaaaat caacggtcag gttattacga tcaaaggtaa aaacggcgag ctgactcgta
     7741 ctctcaacga tgctgttgaa gttaaacatg cagataatac cctgaccttc ggtccgcgtg
     7801 atggttacgc agacggttgg gcacaggctg gtaccgcgcg tgccctgctg aactcaatgg
     7861 ttatcggtgt taccgaaggc ttcactaaga agctgcagct ggttggtgta ggttaccgtg
     7921 cagcggttaa aggcaatgtg attaacctgt ctctgggttt ctctcatcct gttgaccatc
     7981 agctgcctgc gggtatcact gctgaatgtc cgactcagac tgaaatcgtg ctgaaaggcg
     8041 ttgataagca ggtgatcggc caggttgcag cggatctgcg cgcctaccgt cgtcctgagc
     8101 cttataaagg caagggtgtt cgttacgccg acgaagtcgt gcgtaccaaa gaggctaaga
     8161 agaagtaagg taacactatg gataagaaat ttgctcgtat ccgtcgtgcg acccgcgcac
     8221 gccgcaagct ccaggagctg ggcgcaactc gcctggtggt acatcgtacc ccgcgtcaca
     8281 tttacgcaca ggtaattgca ccgaacggtt ctgaagttct ggtagctgct tctactgtag
     8341 aaaaagctat cgctgaacaa ctgaagtaca ccggtaacaa agacgcggct gcagctgtgg
     8401 gtaaagctgt cgctgaacgc gctctggaaa aaggcatcaa agatgtatcc tttgaccgtt
     8461 ccgggttcca atatcatggt cgtgtccagg cactggcaga tgctgcccgt gaagctggcc
     8521 ttcagttcta aggtagaggt gtaagatggc tcacatcgaa aaacaagctg gcgaactgca
     8581 ggaaaagctg atcgcggtaa accgcgtatc taaaaccgtt aaaggtggtc gtattttctc
     8641 cttcacagct ctgactgtag ttggcgatgg taacggtcgc gttggttttg gttacggtaa
     8701 agcgcgtgaa gttccagcag cgatccagaa agcgatggaa aaagcccgtc gcaatatgat
     8761 taacgtcgcg ctgaataacg gcactctgca acaccctgtt aaaggtgttc acacgggttc
     8821 tcgcgtattc atgcagccgg cttccgaagg taccggtatc atcgccggtg gtgcaatgcg
     8881 cgccgttctg gaagtcgctg gggttcataa cgttctggct aaagcatatg gttccaccaa
     8941 cccgatcaac gtggttcgtg caactattga tggcctggaa aatatgaatt ctccagaaat
     9001 ggtcgctgcc aagcgtggta aatccgttga agaaattctg gggaaataaa ccatggcaaa
     9061 gactattaaa attactcaaa cccgcagtgc aatcggtcgt ctgccgaaac acaaggcaac
     9121 gctgcttggc ctgggtctgc gtcgtattgg tcacaccgta gagcgcgagg atactcctgc
     9181 tattcgcggt atgatcaacg cggtttcctt catggttaaa gttgaggagt aagagatgcg
     9241 tttaaatact ctgtctccgg ccgaaggctc caaaaaggcg ggtaaacgcc tgggtcgtgg
     9301 tatcggttct ggcctcggta aaaccggtgg tcgtggtcac aaaggtcaga agtctcgttc
     9361 tggcggtggc gtacgtcgcg gtttcgaggg tggtcagatg cctctgtacc gtcgtctgcc
     9421 gaaattcggc ttcacttctc gtaaagcagc gattacagcc gaagttcgtc tgtctgacct
     9481 ggctaaagta gaaggcggtg ttgtagacct gaacacgctg aaagcggcta acattatcgg
     9541 tatccagatc gagttcgcga aagtgatcct ggctggcgaa gtaacgactc cggtaactgt
     9601 tcgtggcctg cgtgttacta aaggcgctcg tgctgctatc gaagctgctg gcggtaaaat
     9661 cgaggaataa gtagcagatg gctaaacaac cgggattaga ttttcaaagt gccaaaggtg
     9721 gcttaggcga gctgaaacgc agactgctgt ttgttatcgg tgcgctgatt gtgttccgta
     9781 ttggctcttt tattccgatc cctggtattg atgccactgt acttgccaaa ctgcttgagc
     9841 aacagcgagg caccatcatt gagatgttta acatgttctc tggtggtgct ctcagccgtg
     9901 cttctatctt tgctctgggg atcatgccgt atatttcggc gtcgatcatt atccagctgc
     9961 tgacggtggt tcacccaacg ttggcggaaa ttaagaaaga aggggagtct ggtcgtcgta
    10021 agatcagcca gtacacccgc tacggtactc tggtgctggc aatattccag tcgatcggta
    10081 ttgctactgg tctgccgaat atgcctggta tgcaaggcct ggtgattaac ccgggctttg
    10141 cattctactt caccgctgtt gtaagtctgg tcacaggaac gatgttcctg atgtggttgg
    10201 gcgaacagat tactgaacga ggtatcggca acggtatttc aatcattatc ttcgtcggta
    10261 ttgtcgcggg actcccgcca gccattgccc atactatcga gcaagcgcgt caaggcgacc
    10321 tgcacttcct cgtgttgctg ttggttgcag tattagtatt tgcagtgacg ttctttgttg
    10381 tatttgttga gcgtggtcaa cgccgcattg tggtaaacta cgcgaaacgt cagcaaggtc
    10441 gtcgtgttta tgctgcacag agcacacatt taccgctgaa agtgaatatg gcgggggtaa
    10501 tcccggcaat cttcgcttcc agtattattc tgttcccggc gaccatcgcg tcatggttcg
    10561 ggggcggtac tggttggaac tggctgacaa caatttcgct gtatttgcag cctgggcaac
    10621 cgctttatgt gttactctat gcgtctgcaa tcatcttctt ctgtttcttc tacacggcgt
    10681 tggttttcaa cccgcgtgaa acagcagata acctgaagaa gtccggtgca tttgtaccag
    10741 gaattcgtcc gggagagcaa acggcgaagt atatcgataa agtaatgacc cgcctgaccc
    10801 tggttggtgc gctgtatatt acctttatct gcctgatccc ggagttcatg cgtgatgcaa
    10861 tgaaagtacc gttctacttc ggtgggacct cactgcttat cgttgttgtc gtgattatgg
    10921 actttatggc tcaagtgcaa actctgatga tgtccagtca gtatgagtct gcattgaaga
    10981 aggcgaacct gaaaggctac ggccgataat tggtcgcccg agaagttacg gagagtaaaa
    11041 atgaaagttc gtgcttccgt caagaaatta tgccgtaact gcaaaatcgt taagcgtgat
    11101 ggtgtcatcc gtgtgatttg cagtgccgag ccgaagcata aacagcgcca aggctgattt
    11161 tttcgcatat ttttcttgca aagttgggtt gagctggcta gattagccag ccaatctttt
    11221 gtatgtctgt gcgtttccat ttgagtatcc tgaaaacggg cttttcagca tggaacgtac
    11281 atattaaata gtaggagtgc atagtggccc gtatagcagg cattaacatt cctgatcata
    11341 agcatgccgt aatcgcatta acttcgattt atggcgtcgg caagacccgt tctaaagcca
    11401 tcctggctgc agcgggtatc gctgaagatg ttaagatcag tgagctgtct gaaggacaaa
    11461 tcgacacgct gcgtgacgaa gttgccaaat ttgtcgttga aggtgatctg cgccgtgaaa
    11521 tcagcatgag catcaagcgc ctgatggatc ttggttgcta tcgcggtttg cgtcatcgtc
    11581 gtggtctccc ggttcgcggt cagcgtacca agaccaacgc acgtacccgt aagggtccgc
    11641 gcaaaccgat caagaaataa tcggggtgat tgaataatgg caaaggcacc aattcgtgca
    11701 cgtaaacgtg taagaaaaca agtctctgac ggcgtggctc atatccatgc ttctttcaac
    11761 aacaccatcg tgactatcac tgatcgtcag ggtaacgcgt tgggttgggc aacagccggt
    11821 ggttccggtt tccgtggttc tcgcaaatcc actccgtttg cagctcaggt tgcagcagag
    11881 cgttgcgctg acgccgtgaa agaatacggc atcaagaatc tggaagttat ggttaaaggt
    11941 ccgggtccag gccgcgaatc tactattcgt gctctgaacg ccgcaggttt ccgcatcact
    12001 aacattactg atgtgactcc gatccctcat aacggttgtc gtccgccgaa aaaacgtcgc
    12061 gtataacgcc tcgttttcca ggtttgttgg agaaagaaaa tggcaagata tttgggtcct
    12121 aagctcaagc tgagccgtcg tgagggcacc gacttattcc ttaagtctgg cgttcgcgcg
    12181 atcgatacca agtgtaaaat tgaacaagct cctggccagc acggtgcgcg taaaccgcgt
    12241 ctgtctgact atggtgtgca gttgcgtgaa aagcaaaaag ttcgccgtat ctatggtgtg
    12301 ctggagcgtc agttccgtaa ctactacaaa gaagcagcac gtctgaaagg caacaccggt
    12361 gaaaacctgt tggctctgct ggaaggtcgt ctggacaacg ttgtataccg tatgggcttc
    12421 ggtgccactc gtgcagaagc acgtcagctg gttagccata aagcaattat ggtaaacggt
    12481 cgtgttgtta acatcgcttc ttatcaggtt agtccgaatg acgttgtaag cattcgtgag
    12541 aaagcgaaga agcagtctcg cgtgaaagcc gctctggagc tggctgagca gcgtgaaaag
    12601 ccaacctggc tggaagttga tgctggcaag atggaaggta cgtttaagcg taagccggag
    12661 cgttctgatc tgtctgcgga cattaacgaa cacctgatcg tcgagcttta ctccaagtaa
    12721 agcttagtac caaagagagg acacaatgca gggttctgtg acagagtttc taaaaccgcg
    12781 cctggttgat atcgagcaag tgagttcgac gcacgccaag gtgacccttg agcctttaga
    12841 gcgtggcttt ggccatactc tgggtaacgc actgcgccgt attctgctct catcgatgcc
    12901 gggttgcgcg gtgaccgagg ttgagattga tggtgtacta catgagtaca gcaccaaaga
    12961 aggcgttcag gaagatatcc tggaaatcct gctcaacctg aaagggctgg cggtgagagt
    13021 tcagggcaaa gatgaagtta ttcttacctt gaataaatct ggcattggcc ctgtgactgc
    13081 agccgatatc acccacgacg gtgatgtcga aatcgtcaag ccgcagcacg tgatctgcca
    13141 cctgaccgat gagaacgcgt ctattagcat gcgtatcaaa gttcagcgcg gtcgtggtta
    13201 tgtgccggct tctacccgaa ttcattcgga agaagatgag cgcccaatcg gccgtctgct
    13261 ggtcgacgca tgctacagcc ctgtggagcg tattgcctac aatgttgaag cagcgcgtgt
    13321 agaacagcgt accgacctgg acaagctggt catcgaaatg gaaaccaacg gcacaatcga
    13381 tcctgaagag gcgattcgtc gtgcggcaac cattctggct gaacaactgg aagctttcgt
    13441 tgacttacgt gatgtacgtc agcctgaagt gaaagaagag aaaccagagt tcgatccgat
    13501 cctgctgcgc cctgttgacg atctggaatt gactgtccgc tctgctaact gccttaaagc
    13561 agaagctatc cactatatcg gtgatctggt acagcgtacc gaggttgagc tccttaaaac
    13621 gcctaacctt ggtaaaaaat ctcttactga gattaaagac gtgctggctt cccgtggact
    13681 gtctctgggc atgcgcctgg aaaactggcc accggcaagc atcgctgacg agtaaccgga
    13741 tcacaggtta aggttttact gagaaggata aggtcatgcg ccatcgtaag agtggtcgtc
    13801 aactgaaccg caacagcagc catcgccagg ctatgttccg caatatggca ggttcactgg
    13861 ttcgtcatga aatcatcaag acgactctgc ctaaagcgaa agagctgcgc cgcgtagttg
    13921 agccgctgat tactcttgcc aagactgata gcgttgctaa tcgtcgtctg gcattcgccc
    13981 gtactcgtga taacgagatc gtggcaaaac tgtttaacga actgggcccg cgtttcgcga
    14041 gccgtgccgg tggttacact cgtattctga agtgtggctt ccgtgcaggc gacaacgcgc
    14101 cgatggctta catcgagctg gttgatcgtt cagagaaagc agaagctgct gcagagtaat
    14161 ctgaagcaat gtaaaaaaac ccgccccggc gggttttttt atacccgtag tatccccact
    14221 tatctacaat agctgtactc tttttgttca tcccctggag tatttatgtg gttacttgac
    14281 cagtgggcag agcgccatat agcagaagcg caagcgaaag gtgagtttga taacctggca
    14341 ggtagcggcg aaccattgat actggatgat gattctcacg tgccaccgga attacgtgcg
    14401 gggtatcgct tgctgaagaa tgccggttgc ttaccgccag aacttgagca acggagagaa
    14461 gcaattcagc ttctggatat tctcaaaggt atccgtcacg atgatccgca atatcaagag
    14521 gttagccgtc gattgtcatt actggaattg aagctgcgac aagctggatt gagtaccgat
    14581 tttttacgcg gcgattatgc tgacaagttg ttggacaaaa tcaacgataa ctagtggagt
    14641 atgtatgtat cgcattggtg agctggcaaa aatggcggaa gtaacacctg acacgattcg
    14701 ttattacgaa aaacagcaga tgatggagca tgaagtgcgt actgaaggcg ggtttcgcct
    14761 gtataccgaa agcgatctcc agcgattgaa atttatccgc catgccagac aactaggttt
    14821 cagtctggag tcgatccgcg agttgctgtc gatccgcatc gatcctgaac accatacctg
    14881 tcaggagtca aaaggcattg tgcaggaaag attgcaggaa gtcgaagcac ggatagccga
    14941 gttgcagagt atgcagcgtt ccttgcaacg ccttaacgat gcctgttgtg ggaccgccca
    15001 tagcagtgtt tattgctcga ttcttgaagc tcttgaacaa ggggcgagtg gcgttaagag
    15061 tggctgttga ttttttgcac tgacaggatt acactcgcgc cgttaaataa ccaactggag
    15121 tttttatgag ccgatatcag catactaaag ggcagataaa ggataatgcg atagaagcat
    15181 tactacatga tcccttattc cgacagcgag tagagaaaaa taagaaaggg aaaggcagtt
    15241 acatgcgaaa aggaaaacat ggcaatcggg ggaactggga ggccagtggc aagaaagtaa
    15301 atcacttttt taccactggt cttctgcttt caggcgcttg ttaagagcgg ttattctgct
    15361 ctttcagcaa atcacgaatt tctgccagta atacttcttc tttagttggt gcaggtgcgg
    15421 cagctggttc ttcttttttc cgattcagtt tgttgattag cttaatcgcc ataaagatgg
    15481 caaaggccac aatcagaaaa tcaaagacgt tttgaatgaa gacaccgtaa tgcatcacaa
    15541 cagcagggat atccccctgc gcatcgcgta gcgtgacagc aaactgttta aagtcgatcc
    15601 cgccaattaa taagcccaga ggtggcatga tgatatcggc aaccagtgaa gatacaatct
    15661 tcccgaatgc cgcaccgata atgacaccca ccgccaaatc caccacgttc ccgcgcatcg
    15721 caaattcgcg aaattcttta ataatgctca tgttattctc cctatgaagc cgacaaccat
    15781 aagtctaaca aatgttaagc cattttcctg ccaggaatga atttaattaa tccttgataa
    15841 tcaaagggaa ataataaggc gtcattagac gccttattaa ttacaagaag aaagggcttg
    15901 gctggaagag tctttcgacg tcggtaataa actttttatc tgtgaggaac ataattacgt
    15961 gatcgccttg ctcaatgcgc agattgtcat tggcaatcat cacgtcgttt ccacgtacca
    16021 ccgctccaat aatcgttcct ggcggtagct tgatttcgtc aatgactctg ccgacaacgc
    16081 gtgaggtgct ttcatcaccg tgagcaacgg cttcaatagc ttctgctacg ccgcggcgca
    16141 atgaggaaac accaacaata tctgcttttc gcacatggct aagcaacgca gaaatagttg
    16201 cttgttgtgg tgaaattgca atatcgatca cgctcccctg aaccagatcc acataagcgc
    16261 gacgctggat caataccatc acctttttcg cacccatacg tttggcaagc atggcggaca
    16321 tgatattggc ctcgtcatcg ttggtgacgg caataaacag atcaacttga tcgatatgtt
    16381 cttcggccag cagttcttga tccgacgcat caccaaaaaa gacgatcgta ttctgcaact
    16441 tttccgccag ttcggcagcg cgctgctgat tacgttcgat gagtttaacg ctgtaatctt
    16501 tttccagacg acgcgccagc cctgcaccga tattaccgcc accaaccagc atgatccgct
    16561 tatacggttt ttccagtcgc tgtaattcac tcatcaccgc gcggatatgc tgtgaagcgg
    16621 caataaagaa cacttcatca ccagcttcaa caatggtcga accttgtgga cgaatggggc
    16681 gatcgtggcg gaaaattgct gccacacgag tatcgatatg tggcatatgt tcgcgcatgg
    16741 tcgaaagtgc attaccaatc agcgggccgc cataataggc tttaactaca gcgaggctga
    16801 ctttaccctc agcgaagttc accacctgca atgcgcctgg atactcaatc agtcggtaaa
    16861 tattatcgat aaccaactgc tctggtgcga tcagatgatc aatcggtaca gcatctgaat
    16921 gaaatagctt atcggcatcg cgtacgtagt ctggtgagcg gatgcgagcg atgcgattag
    16981 gggtgttgaa aagtgagtag gctacctggc aggcaaccat attggtttca tctgaactgg
    17041 ttacagcaac cagcatatcg gcgtcgtcgg cacctgcctc ccgcaatacg cgtggatgag
    17101 agccatgccc ctgcacgact cgcaggtcaa atttatcctg caaggtccgc agacgctcac
    17161 cgttggtatc gacaacagta atatcgttgt tctcgccaac caggttttcc gccagtgtgc
    17221 cgccaacctg gccggcaccc agaatgataa ttttcatcag tctcgacccg ttatctcatc
    17281 actttttgat tagcttagcg taaaagaagc cgtcgccctc ttcggcacca ggcagatttt
    17341 gtttacccgg ttgctctggt gttcctgttt cgcaaagttc ggcatcagcg gtacgttgca
    17401 aaaaagcttt aatctgcagg ctattctctt ccggtaacac cgaacaggtg gcatagacca
    17461 gggttccacc ggattttaaa tgcggccaaa tggcgtcgag aatttcagac tgcaattgcg
    17521 cgagttccgg gatatcgcga tcgcgacgta accatttaat atccggatgg cgacgaatca
    17581 cccccgttgc tgaacaaggc gcatctaata aaatgcgatc aaactgttgc tcgccacacc
    17641 attgggaagg gtaacggcca tcaccttgtt tcacggtcgc cttcatacca aggcgtttta
    17701 aattgtcgtg aacgcgagag aggcgctgtt cgtcaatatc aaccgcaaca acctgcgctt
    17761 ctggtgccac ctcaaggata tgcgttgttt taccgccggg ggcggcacaa agatccagaa
    17821 tgtgttcacc gttttgtggc gcaagccagg tcatgcaacc ttgtgctgat gcatcctgaa
    17881 cggtaaccca tccgtcttca aaaccaggta gcgcatgaac aggtgcaggt gtttccagcc
    17941 gtacagcatc agggtaatcc gcatgcggga aacctttcat tcctgcttca tccaacaatg
    18001 caagccagct gtcgcgggaa tgatgcgtac gattaacgcg cagccacatt ggcggacgct
    18061 ggttattggc ttcgacgatg gattgccact gctctggata cgctttttgc agacgcttca
    18121 gcaaccagga aggatgcaga taacgtgcat cactggtatt aaactcggct aataactctt
    18181 cttgctgatg ctggaactgg cgtaatacac cgttaatcaa ccctttaagt tgcggacgct
    18241 taattgcgat agcgccttca accgtttcag ccagcgcagc atgaggtgga atgcgggtat
    18301 aaagcagttg atacaaacca accataatca ggtaatgcac agtgcgctgt ttgccggtca
    18361 tcggacgggc cattaactta ttaatcagcc agtctaactg cgaaagcgta cgcagtacgc
    18421 caaagcacaa ctcttgaaga agtgctttgt ctttatcaga aactttttgc tggagcggtg
    18481 gcagaatgtt gcttaatgat tgcccttgct cgacgacttg ttcaacggcc tgggccgcca
    18541 tgctacgtaa attacgttgt tttttcataa gtataaaaat gcccggtaag accgggctta
    18601 gaagagtgga ctatcaggcc agacggttgc ccggaacaaa ccattcccga cgagaattca
    18661 ggaggtcttg cgcgctcatc gctttcttac ccgcaggttg taacgagagc aggttcagga
    18721 tgccatcacc agtcgcaacc tgaatgcctt gtttgttggc ttcaaggatc gttcctggtg
    18781 cagcgttggt tgccgtatca atgaccgacg ctttccagac tttaaccggt tgtccttcaa
    18841 tttccagcca gctcattggc catgggttga aagcgcgaat gcagcgttca agctgcgctg
    18901 ccgaaagcga ccagtcaata cgcgcttctt ctttactcaa cttctcggcg taagtgacaa
    18961 gagtttcgtc ctgaacttct ggtttcgccg tgccgtctgc tagttgtttc aacgtggtga
    19021 ttagcccttg tgggccaagc tctgccagct tgtcgtacag cgtaccactg gtatcttctg
    19081 cagtaatcgg gcaggagagc ttatagagca tatcaccggt gtctaaaccg acatccattt
    19141 gcataatggt cacaccagtt tctgcatcac ccgcccatag tgagcgttgg attggtgcag
    19201 caccgcgcca gcgtggcagc agtgaaccat gaacgttgat acagccaaga cgcggcatct
    19261 ccagcactgc tttaggcaga attaagccat aggcgacgac gaccataaca tcagcctgca
    19321 gatcggcgac cagttgctgg ctttcttgtg gacgcaggga aacaggttga aaaacgggca
    19381 gacctttttc ctcagccaga actttaaccg ggctgggcat cagtttttta ccgcgtcctg
    19441 ccggtcggtc tggctgggtg aacacgccaa cgacgttatg accagaagac aacagcgcgt
    19501 cgagatgacg cgctgcaaag tcaggtgtac ccgcaaaaat aatacgtagt gattctgaca
    19561 cgttagttct tatccttaag cccgggcttt cagacgatct agtttttcaa ctttctgacg
    19621 aatacgttgt tgtttcagcg gtgacagata atccataaac agtttgccga ccaggtgatc
    19681 catctcatgc tgaatacaga tggctaacag cccctctgct tccagttcaa atggtttacc
    19741 gtcgcggtca agggcgcgaa ttttaacttt ctctgcgcgc ggcactaaag cacgttgttc
    19801 agggatcgac aggcaacctt cttcaatgcc tgtttcgccg cttttttcta aaagctccgg
    19861 attgattaac accagccgtt cgtcacggtt ttccgaaaca tcaataacaa tgatacgttg
    19921 atggatatca acctgggttg ccgccaggcc aataccttct tctgcgtaca tcgtctcgaa
    19981 catatcatcg acgatacgct gtatttctgc attcacttct tctaccggtt tagcaacttt
    20041 gcgaagccgc tcgtccggaa tatgtaacac ttgcaaaact gacataaatc tccagagatg
    20101 tgttcagggg ttagaaagat tatttcttct attctagaca aatccccctc tgattgacag
    20161 catcactgac caatcgcaaa gattgctaag gctgcttatg gcagggagat aaggatggtc
    20221 gatacagaaa tttggctgcg tttaatgagt atcagcagct tgtacggcga tgatatggtc
    20281 cgtatagctc actggctggc aaaacagtcg caaattgatg cggttggatt gcagcaaaca
    20341 gggcttacat tgcggcaggc acaacgcttt ctttcatttc cgcgaaagag tatcgaaagc
    20401 tcactttgtt ggttggagca acccaaccat catttaatcc ctgcggacag cgaattttat
    20461 cctcctcaac ttctggtgac gacagattac cccggcgcac tgtttgttga aggagaactg
    20521 cacgcgctgc attcatttca gcttgccgta gtggggagtc gggcgcattc atggtatggc
    20581 gagcgatggg gacggttatt ttgcgaaact ctggcgacgc gtggagtgac aattacgagt
    20641 ggactggcgc gtggaatcga tggtgtggcg cataaagctg ccttacaggt aaatggcgtc
    20701 agcattgctg tattggggaa tggacttaat accattcatc cccgccgcca tgcccgactg
    20761 gctgccagtc tgcttgaaca tggcggagct ctcgtctcgg aatttcccct cgatgttcca
    20821 ccccttgctt acaatttccc acgaagaaat cgcattatca gtggtctaag taaaggtgta
    20881 ctggtggtgg aagcggcttt gcgcagtggt tcgctggtga cagcacgttg tgcgcttgag
    20941 cagaggcgtg aagtttttgc cttgccaggt ccaataggga atccgggaag cgaagggcct
    21001 cactggttaa taaaacaagg tgcgattctt gtgacggaac cggaagaaat tctggaaaac
    21061 ttgcaatttg gattgcactg gttgccagac gcccctgaaa attcatttta ttcaccagat
    21121 cagcaagacg tggcattgcc atttcctgag ctcctggcta acgtaggaga tgaggtaaca
    21181 cctgttgacg tcgtcgctga acgtgccggc caacctgtgc cagaggtagt tactcaacta
    21241 ctcgaactgg agttagcagg atggatcgca gctgtacccg gcggctatgt ccgattgagg
    21301 agggcatgcc atgttcgacg tactaatgta tttgtttgaa acctatattc acactgaagc
    21361 tgagttgcgt gtggatcaag acaaacttga acaggatctt accgacgcag gttttgagcg
    21421 agaagatatc tacaatgccc tgctatggct ggaaaaactt gctgattatc aggaagggtt
    21481 ggcagaaccg atgcaactgg cctcggatcc tctctccatg cgtatttata caccggaaga
    21541 gtgtgaacga ctggatgcca gctgccgtgg gtttctgctt ttccttgagc agattcaggt
    21601 gctcaacctt gaaactcgtg aaatggtgat agagcgagtg ctcgcgctgg ataacgcaga
    21661 gttcgaactg gatgatctga aatgggtgat cctgatggtg ttgttcaaca tcccggggtg
    21721 cgaaaatgcg taccagcaaa tggaagaatt actctttgaa gtgaatgaag gtatgctgca
    21781 ttaatcattt ctttaattca gcataagttg ttatggcaaa atcagcactg ttcacggtgc
    21841 gtaataatga gtcctgccca aagtgcgggg ctgaactggt tattcgatcc gggaaacacg
    21901 gtccgtttct tggatgctca cagtatccgg cgtgtgacta cgtccgtctt ctgaaatctt
    21961 cagcggatgg acatattgtc aaagttctgg aggggcaggt ttgccctgca tgtggcgcaa
    22021 atctggtatt acgccaggga cgctttggta tgtttattgg ttgcagtaac taccctgaat
    22081 gcgaacatac cgaacttatc gataaaccgg acgaaacagc aattacatgc ccccaatgtc
    22141 ggacgggcca tctggtccag cgccgctccc gttatggcaa aacatttcac tcctgtgatc
    22201 gctacccgga gtgtcaattt gccattaact tcaaaccaat agctggagaa tgccctgagt
    22261 gtcattatcc gctactcatc gaaaagaaaa ccgcgcaggg tgtaaaacac ttttgtgcca
    22321 gtaaacaatg tggaaagccg gtttcggcgg aataataacg tgaataataa cctgcaagga
    22381 gacgctatcg cagctgcgat agatgtcctc aatgaagaac gtgtcatcgc ctatccaacg
    22441 gaagccgttt tcggtgttgg gtgcgatcct gatagcgaaa cagcagtgat gagactgttg
    22501 gagttaaaac agcgtccggt tgataagggg ctgattttaa tcgcggcaaa ttacgagcag
    22561 cttaaaccct atattgatga caccatgctg actgatgcgc agcgtgaaac cattttttcc
    22621 cgctggccag gtcctgtcac ctttgtcttt cccgcgcctg cgacaacacc gcgctggttg
    22681 acgggccgct ttgattcgct tgctgtacga gtcaccgacc atccgttggt ggttgctttg
    22741 tgccaggctt atggtaaacc gctggtttct accagtgcca acttgagtgg attgccacct
    22801 tgtcgaacag tagacgaagt tcgcgcacaa tttggcgcgg cgttcccggt tgtgcctggt
    22861 gaaacggggg ggcgtttaaa tccttcagaa atccgcgatg ccctgacggg tgaactgttt
    22921 cgacaggggt aacataatgg aaacctatgc tgtttttggt aatccgatag cccacagcaa
    22981 atcgccattc attcatcagc aatttgctca gcaactgaat attgaacatc cctatgggcg
    23041 cgtgttggca cccatcaatg atttcatcaa cacactgaac gctttctttc gtgctggtgg
    23101 taaaggtgcg aatgtgacgg tgccttttaa agaagaggct tttgccagag cggatgagct
    23161 tactgaacgg gcagcgttgg ctggtgcagt taataccctc aagcggttag aagatggacg
    23221 cctgctgggt gacaataccg atggtgtagg cttgttaagc gatctggaac gtctgtcttt
    23281 tatccgccct ggtttacgta ttctgcttat cggcgctggt ggagcatctc gcggcgtact
    23341 actgccactt ctttccctag actgtgcggt gacaatcact aatcggacgg tatcccgcgc
    23401 ggaagagttg gctaaattgt ttgcgcacac tggcagtatt caggcgttgg gtatggacga
    23461 actggaaggt catgagtttg atctcattat taatgcaaca tccagtggca tcagtggtga
    23521 tattccggcg atcccgtcat cgctcattca tccaggcatt tattgctatg acatgttcta
    23581 tcagaaagga aaaactcctt ttctggcatg gtgtgagcag cgaggctcaa agcgtaatgc
    23641 tgatggttta ggaatgctgg tggcacaggc ggctcatgcc ttccttcttt ggcatggcgt
    23701 tctgcctgat gtagaaccgg tcatcaaact attgcaacag gaattgtcag cgtgaatcag
    23761 gccatccagt ttccggatag ggaagagtgg gacgagaata aaagatgtgt atgttttcct
    23821 gctctcgtga atggtatgca actgacatgt gcgatctctg gcgagagtct ggcgtatcgc
    23881 tttactggag gtacgccaga acagtggtta gcgagttttc gtcagcatcg ctgggacctg
    23941 gaagaagaag cggaaaactt aattcaggaa caaagtgaag atgatcaagg ttgggtctgg
    24001 ttaccttgat ccagatattc gtccttccat ttcacgtagt tatttgcgga atagcgtaac
    24061 ccagccttct cttcatcact taacgggcgg atctgtttga cgggactacc gagatacaga
    24121 tatccgctct ccagccgttt attttgtggg accaggctac ccgcaccaat catcacatca
    24181 tcttctacta ttgcgccatc aagtaaaatt gagcccatcc caaccaaaac tcgattgcca
    24241 atggtgcagc cgtggagcat caccttgtga ccaacagtga catcttcgcc aatggttaat
    24301 gggttgccat ctgggttgta cgaggattta tgagtgacat gcaacatact gccatcctgg
    24361 atattggtgc gtgctccgat ctgtacataa tgtacatctc cacgaatcac aacgagcggc
    24421 cagatcccca catcatcagc cagacgaacg tcaccaatca cgacgctgct atcgtcgatc
    24481 attacgcgct ggccgatttg tggaaaaaga tcgcggtatg ggcgtaaaac atcagacata
    24541 ctcacctcaa caataaatga tttcctaatg actttgggtg cattattagc cttgtgcaag
    24601 tcttttagta tgcaaaaaag caccgttttg tgtgcgaatg cagcaaaaag ggtgaaaaaa
    24661 caacaaacag aaaaaaagat caaaaaagta cttgtgcaaa aaattgggat ccctataatg
    24721 cgcctccgtt gagacgacaa cgtgaaacac ttcacaggat ggtcggaaca acgaagagaa
    24781 aaaattctga aattcagggt tgactctgaa agaggaaagc gtaatatacg ccacctcgcg
    24841 acagtgagct gaaagccgcg tcgcaactgc tctttaacaa tttatcagac aatctgtgtg
    24901 ggcactcgaa gatacggatt cttaacgtcg caagacgaaa aatgaatacc aagtctcaag
    24961 agtgaacacg taattcatta cgaagtttaa ttctttgagc atcaaacttt taaattgaag
    25021 agtttgatca tggctcagat tgaacgctgg cggcaggcct aacacatgca agtcgaacgg
    25081 taacaggaag cagcttgctg cttcgctgac gagtggcgga cgggtgagta atgtctggga
    25141 aactgcctga tggaggggga taactactgg aaacggtagc taataccgca taatgtcgca
    25201 agaccaaaga gggggacctt cgggcctctt gccatcggat gtgcccagat gggattagct
    25261 tgttggtggg gtaacggctc acc
//
LOCUS       DAZPLI010000048        25105 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZPLI010000048 DAZPLI010000000
VERSION     DAZPLI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25105
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.048"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            195..383
                     /gene="bcsR"
                     /locus_tag="ACHUU3_001904"
     CDS             195..383
                     /gene="bcsR"
                     /locus_tag="ACHUU3_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR2081985.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            395..1147
                     /gene="bcsQ"
                     /locus_tag="ACHUU3_001905"
     CDS             395..1147
                     /gene="bcsQ"
                     /locus_tag="ACHUU3_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR2081986.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1144..1308
                     /locus_tag="ACHUU3_001906"
     CDS             1144..1308
                     /locus_tag="ACHUU3_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HFR2081987.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1315..3762
                     /gene="bcsA"
                     /locus_tag="ACHUU3_001907"
                     /pseudo
     CDS             1315..3762
                     /gene="bcsA"
                     /locus_tag="ACHUU3_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            3773..6112
                     /gene="bcsB"
                     /locus_tag="ACHUU3_001908"
     CDS             3773..6112
                     /gene="bcsB"
                     /locus_tag="ACHUU3_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR2081988.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVLRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6119..7225
                     /gene="bcsZ"
                     /locus_tag="ACHUU3_001909"
     CDS             6119..7225
                     /gene="bcsZ"
                     /locus_tag="ACHUU3_001909"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR2081989.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7207..10699
                     /gene="bcsC"
                     /locus_tag="ACHUU3_001910"
                     /pseudo
     CDS             7207..10699
                     /gene="bcsC"
                     /locus_tag="ACHUU3_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            10781..12769
                     /gene="hmsP"
                     /locus_tag="ACHUU3_001911"
     CDS             10781..12769
                     /gene="hmsP"
                     /locus_tag="ACHUU3_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR2081990.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12952..14238
                     /gene="dctA"
                     /locus_tag="ACHUU3_001912"
     CDS             12952..14238
                     /gene="dctA"
                     /locus_tag="ACHUU3_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR2081991.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14458..15954
                     /locus_tag="ACHUU3_001913"
     CDS             14458..15954
                     /locus_tag="ACHUU3_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR2081992.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16050..16979)
                     /gene="kdgK"
                     /locus_tag="ACHUU3_001914"
     CDS             complement(16050..16979)
                     /gene="kdgK"
                     /locus_tag="ACHUU3_001914"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR2081993.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17211..17978
                     /gene="pdeH"
                     /locus_tag="ACHUU3_001915"
     CDS             17211..17978
                     /gene="pdeH"
                     /locus_tag="ACHUU3_001915"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR2081994.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18048..20108
                     /locus_tag="ACHUU3_001916"
     CDS             18048..20108
                     /locus_tag="ACHUU3_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR2081995.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20290..21612)
                     /locus_tag="ACHUU3_001917"
     CDS             complement(20290..21612)
                     /locus_tag="ACHUU3_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2081996.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22023..23036)
                     /gene="yhjD"
                     /locus_tag="ACHUU3_001918"
     CDS             complement(22023..23036)
                     /gene="yhjD"
                     /locus_tag="ACHUU3_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR2081997.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23085..23984)
                     /locus_tag="ACHUU3_001919"
     CDS             complement(23085..23984)
                     /locus_tag="ACHUU3_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR2081998.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24504..>25105
                     /locus_tag="ACHUU3_001920"
     CDS             24504..>25105
                     /locus_tag="ACHUU3_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR2081999.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5893 a         6366 c         6992 g         5854 t
ORIGIN      
        1 tttagcttgt gaatcaattt agaaggtcac aagacgtgtg attttagtct tttcgttttt
       61 aactttttct gttgcgtaac ggaaagtcaa aaagtgagca aattcccgtc tcgccgtaaa
      121 caataaaccg gcgaaatgct cacggttaga attgtttcat gaacggtacg gttatttcat
      181 agggatcaag caaaatgaat aacaatgaac cagatactct gcctgatccc gcgataggct
      241 atatcttcca gaatgatatt gtggcgttaa agcaggcatt ttcactgcct gatattgatt
      301 atgccgatat ttcccaacgc gaacagttgg ccgcggcatt aaaacgctgg ccgttgctgg
      361 cagagtttgc gcaacaaaag taggggattg gtgaatggcc gtactgggat tgcagggggt
      421 gcggggaggc gtggggacaa caaccatcac cgccgcatta gcctggtcat tacaaatgtt
      481 gggagaaaat gtcctggtgg tcgatgcctg cccggacaac ttgttgcgcc tgtcatttaa
      541 tgttgatttt acccaccgtc agggctgggc cagagcgatg ctggatggcc aggactggcg
      601 tgacgctggg ttgcgctaca cctcgcagct cgatttgctg ccttttggtc agttatccat
      661 tgaagaacaa gaaaatccac agcactggca aacccggctg agcgatattt gctccggctt
      721 acagcaacta aaagccagcg ggcgttacca gtggatttta atcgacttac cgcgtgatgc
      781 ctcgcagata acccaccagc tgctgagttt gtgcgatcac tcgctggcaa tcgtcaatgt
      841 ggatgccaac tgccatatcc gactgcatca gcaagcgctg ccggatggcg cacatatttt
      901 gattaatgac ttccgtattg gcagtcaggt tcaggacgat atttaccagc tttggttgca
      961 aagccagcgc cgattactgc cgatgctcat tcatcgtgat gaagcgatgg ctgaatgcct
     1021 ggcggctaag caaccagtag gtgaatatcg cagtgatgcg ctggcggctg aagagatact
     1081 gacgctggcg aactggtgcc tgttgaacta ctccgggctg aaaacgccag tcgggagtgc
     1141 atcatgagta tcctgacccg gtggttgctt atcccgccgg tcaacgcgcg gcttatcggg
     1201 cgttatcgcg attatcgtcg tcacggtgcg tcggctttca gcgcgacgct cggctgtttc
     1261 tggatgatcc tggcctggat ttttattccg ctggagcacc cgcgctagca gcgtattcgc
     1321 gcagaacata aaaacctgta tccgcatatc aacgcctcgc gtccgcgtcc gctggacccg
     1381 gtccgttatc tcattcaaac atgctggtta ctgatcggta catcgcgcaa agaaacgccg
     1441 aaaccgcgca ggcgggcatt ttcaggtctg cagaatattc gtggacgtta ccatcaatag
     1501 atgaacgagc tgcctgagcg cgttagccat aaaacacagc atcttgatga gaaaaaagag
     1561 ctcggtcatt tgagtgccgg ggcgcggcgg ttgatcctcg gtatcatcgt caccttctcg
     1621 ctgattctgg cgttaatctg cgttactcaa ccgtttaacc cgctggcgca gtttatcttc
     1681 ctgatgctgc tgtggggtgt agcgctgatc gtacggcgga tgccggggcg cttctcggcg
     1741 ctaatgttga ttgtgctgtc gctgaccgtt tcttgccgtt atatctggtg gcgttacacc
     1801 tctacgctga actgggacga tccggtcagc ctggtgtgcg ggcttattct gctcttcgct
     1861 gaaacgtacg cgtggattgt gctggtgctc ggctacttcc aggtagtatg gccgctgaat
     1921 cgtcagccgg tgccattgcc gaaggatatg tcgctgtggc cgtcagtgga tatctttgtc
     1981 ccgacttaca acgaaaatct caatgtggtg aaaaatacca tttacgcctc gctgggtatc
     2041 gactggccga aagacaaact gaacatctgg atcctcgatg acggcggcag ggaagagttt
     2101 cgccagtttg cgcaaaacgt gggggtgaaa tatatcgccc gcaccactca tgaacatgcg
     2161 aaagcgggca acatcaacaa tgcgctgaaa tatgccaaag gcgagttcgt gtcgattttc
     2221 gactgcgacc acgtaccaac gcgatcgttc ctgcaaatga ccatgggctg gttcctgaaa
     2281 gagaaacagc tggcgatgat gcagactcca caccacttct tctcgccgga cccgtttgaa
     2341 cgcaacctgg gacgttttcg taaaacgccg aacgaaggca cgctgttcta tggtctggtg
     2401 caggatggca acgatatgtg ggacgccact ttcttctgcg gttcctatgc ggtgattcgt
     2461 cgtaagccgc tggatgaaat tggcggcatt gctgtcgaaa ccgtgactga agatgcgcat
     2521 acttctctgc gtttgcaccg tcgtggctat acctccgcgt atatgcgtat tccgcaggcg
     2581 gcggggctgg cgaccgaaag tctgtcggcg catatcggtc agcgtattcg ctgggcgcgc
     2641 gggatggtac aaatcttccg tctcgataac ccgctcaccg gtaaaggact gaagtttgct
     2701 cagcggctgt gctacgtcaa cgccatgttc cacttcttgt cgggcattcc acggctgatc
     2761 ttcctgactg cgccgctggc gttcctgctg cttcatgcct acatcatcta tgcgccagcg
     2821 ttgatgatcg ccctattcgt gctgccgcat atgatccatg ccagcctgac caactcgaag
     2881 atccagggca aatatcgcca ctctttttgg agtgaaatct acgaaacggt gctggcgtgg
     2941 tatatcgcac caccgacgct ggtggcgctg attaacccgc acaaaggcaa atttaacgtc
     3001 accgccaaag gtggactggt ggaagaagag tacgtcgact gggtgatctc gcggccctac
     3061 atcttccttg ttctactcaa cctggtgggc gttgcggtag gcatctggcg ctacttctat
     3121 ggcccgccaa ccgagatgct caccgtggtc gtcagtatgg tgtgggtgtt ctacaacctg
     3181 attgttcttg gcggcgcagt tgcggtatcg gtagaaagca aacaggtacg ccgatcgcac
     3241 cgcgtggaga tgacgatgcc cgcggcaatt gcccgcgaag atggtcacct cttctcgtgt
     3301 accgttcagg atttctccga cggtggtttg gggatcaaga tcaacggtca ggcgcagatt
     3361 ctggaagggc agaaagtgaa tctgttgctt aaacgcggtc agcaggaata cgtcttcccg
     3421 acccaggtgg cgcgcgtgat gggtaatgaa gttgggctga aattaatgcc gctcaccacc
     3481 cagcaacata tcgattttgt gcagtgtacg tttgcccgtg cggatacatg ggcgctctgg
     3541 caggacagct acccggaaga taagccgctg gaaagtctgc tggatattct gaagctcggc
     3601 ttccgtggct accgccatct ggcggagttt gcgccttctt cggtgaaggg catattccgt
     3661 gtgctgactt ctctggtttc ctgggttgta tcgtttattc cgcgccaccc ggagcggagc
     3721 gaaacggcac aaccatcgga tcaggctttg gctcaacaat gatgataacg cgatgaaaag
     3781 aaaactattc tggatttgtg cagtggctat ggggatgagt gcgttcccct ctttcatgac
     3841 gcaggcgacg ccagcaacgc aaccactgat caatgctgag ccagctgtag ccgcccagac
     3901 ggaacaaaat ccgcaggtgg ggcaagtgat gccgggcgtg cagggcgctg atgcgccagt
     3961 cgtggcgcag aacggtcctt cgcgtgatgt gaagctgacc tttgcgcaaa ttgcgccgcc
     4021 gccgggcagc atggtgctac gtggcattaa cccgaacggc agcattgagt ttggtatgcg
     4081 cagcgatgaa gtggtgacga aggcgatgct caacctcgaa tacacgccat cgccatcgtt
     4141 actgcctgtc cagtcgcagt taaaggttta tctcaatgat gagctgatgg gcgtgctgcc
     4201 agtgaccaaa gaacagttgg gtaaaaaaac gctggcgcaa atgcccatta acccactgtt
     4261 tattaccgac ttcaaccgtg tgcggctgga gtttgtcggc cattatcagg acgtgtgcga
     4321 aaacccggcc agcaccacgc tttggctgga tgttgggcgg agcagtggac tggatctgac
     4381 ctatcagacc ctgaatgtga agagtgacct gtcacacttc ccggtgccat tctttgaccc
     4441 gcgcgataac cgtactaaca ccttgccgat ggtctttgcg ggtgcgccgg atgttgggct
     4501 gcaacaagca tctgccattg tcgcctcgtg gtttggttcg cgttctggct ggcgtgggca
     4561 gaacttcccg gtgctctata accaattgcc ggatcgcaat gccattgtct ttgcaaccaa
     4621 cgacaaacgg ccggacttcc tgcgcgatca tccggcggta aaagccccgg tgattgagat
     4681 gattaaccat ccgcagaatc cttacgtcaa actgctggtg gtgtttggtc gtgacgacaa
     4741 agacctgttg caggcagcga aaggtatcgc tcagggtaac attctgttcc gtggtgaaag
     4801 cgtggtagtg aatgaagtga aaccgctgct accgcgtaag ccgtacgatg cgccgaactg
     4861 ggtacgtacc gatcgtccgg tcacatttgg cgaactgaaa acctatgaag aacagttaca
     4921 atccagcggt cttgagccag cagcgattaa cgtttcgcta aacctgccgc cggatctcta
     4981 cctgatgcgc agtaccggca ttgatatgga tattaattac cgctacacca tgccgccggt
     5041 gaaagacagt tcgcggatgg atatcagcct gaataaccag ttcctgcaat ccttcaacct
     5101 gagcagtaaa caggaggcga accgcctgct gctgcggatt ccggtattac aaggtttgct
     5161 ggatggcaaa acggatgtct ctattccggc gctgaaactg ggcgcgacca accagctgcg
     5221 cttcgacttt gagtatatga acccgatgcc gggcggttcg gtggataact gtattacctt
     5281 ccagccggtg cagaatcatg tggtgattgg tgacgattcc accatcgact tctcgaagta
     5341 ttaccacttc atcccgatgc cggatctacg cgcctttgct aacgcgggct tcccgttcag
     5401 ccggatggcg gatctgtcgc aaaccatcac cgtgatgccg aaaacgccta acgaagcaca
     5461 gatggaaacg ttgctgaata ctgttggttt tatcggcgca cagacgggct tcccggcgat
     5521 taatctgacg gtgaccgatg atggcagcac cattcagggc aaagatgccg acatcatgat
     5581 catcggtggt atcccggaca aattaaaaga cgataagcag atcgacctgt tggtgcaggc
     5641 gaccgaaagc tgggtgaaaa caccgatgcg ccagaccccg ttccccggca ttgtgccgga
     5701 cgagagcgat cgcgcggcag aaacccagtc aacgctgacc tcttccggtg cgatggcggc
     5761 ggtgattggc ttccagtcgc cgtataacga ccagcgcagc gtgattgcgc tgctggcaga
     5821 tagcccacgc ggttatgaaa tgcttaacga tgcggtgaac gatagcggca aacgcgccac
     5881 catgttcggt tcggtcgcgg tgctccgcga gtccggtatc aacagcctac gtgttggcga
     5941 cgtttattac gtaggtcatc tgccgtggtt cgagcgcttg tggtatgcgc tggcaaacca
     6001 tccgattctg ctggcggtgc tggcggcaat cagtgtgata ttgctggcat gggtactgtg
     6061 gcgtctgctg cgaattatta gtcgtcgtcg tcttaacccg gataacgagt aattgaagat
     6121 gaatgtgttg cgtagtggaa tcgtgacgat gctgctgttg gctgccttta gtgttcaggc
     6181 agcctgtacc tggcctgcct gggagcagtt taaaaaggat tacatcagtc aggaagggcg
     6241 cgtcatcgcc cccagcgacg cgcgcaaaat caccacctcc gaagggcaaa gttacggtat
     6301 gttctttgcc ctggcggcta acgaccgtgt agctttcgat aatattctcg actggacgca
     6361 gaacaatctc gctcagggct ctttaaaaga acgtttgccc gcctggctgt ggggcaagaa
     6421 agagaacagt aagtgggaag tgctggacag caattcggcc tccgatggtg atgtctggat
     6481 ggcctggtcg ttgctggagg cggggcgttt gtggaaagag cagcgttata ccgacatcgg
     6541 cagcgcgttg ctaaaacgta tcgcgcggga ggaagtggtg acggtgcctg ggctgggttc
     6601 catgttgtta ccgggcaaag tgggttttgc tgaggataac agctggcgtt ttaaccccag
     6661 ctacctgccg ccgacgctgg cgcagtattt cacccgcttt ggcgcgccgt ggaccacgct
     6721 gcgcgaaacc aatcaacgtt tattgctgga aaccgccccg aaaggttttt cgccagactg
     6781 ggtgcgctat gagaaagaca aaggctggca gctaaaagcc gaaaaaacat tgatcagcag
     6841 ctacgacgct atccgcgttt acatgtgggt aggcatgatg cctgacagcg atccgcaaaa
     6901 agcgcggatg ctcaaccggt ttaaaccgat ggcgacattc actgagaaaa acggttatcc
     6961 gccggaaaaa gtggatgtgg ctacggggaa agcgcagggt aaaggaccga tcggtttttc
     7021 tgccgccatg ctgccctttt tacaaaaccg cgatgcgcag gccgttcagc gccagcgcgt
     7081 ggccgataac tttcccggca gcgatgccta ttacaactat gtgctgaccc tgtttggaca
     7141 aggctgggat caacaccgtt tccgcttctc gacaaaaggt gagttattac ctgactgggg
     7201 ccaggaatgc gcaaattcac actaaacata ttcacgcttt ccctcggtct ggccgtcatg
     7261 ccgatggtcg aggcagcacc aaccgctcag caacagttgc tggagcaagt tcggttaggc
     7321 gaagcgaccc atcgtgaaga tctggtgcaa cagtcgttat atcggctgga acttattgat
     7381 ccgaataacc cggacgtcgt tgccgcccgt ttccgttctt tgttacgtca gggcgatatt
     7441 gatggcgcgc aaaaacagct cgatcggctg tcgcagttag cgccgagttc aaatgcgtat
     7501 aaatcgtcgc ggactacgat gctactttcc acgccggatg gtcgtcaggc actgcaacag
     7561 gcacgattgc aggcgacgac cggtcatgca gaagaagctg tggcgagtta caacaaacta
     7621 ttcaacggtg cgccgccgga aggtgacatt gctgtcgagt actggagtac ggtggcgaaa
     7681 attccggctc gccgtggcga agcgattaat cagttaaaac gcatcaatgc ggatgcaccg
     7741 ggcaatacgg gcctgcaaaa caatctggcg ctattgctgt ttagtagcga tcgccgtgac
     7801 gaaggttttg ccgtcctgga acagatggca aaatcgaacg ccgggcgcga aggggcctct
     7861 aaaatctggt acgggcagat taaagacatg cccgtcagtg atgccagtgt gtcggcgctg
     7921 aaaaaatatc tctcgatctt tagtgatggc gatagcgtgg cggctgcgca atcgcaactg
     7981 gcagaacagc aaaaacagct ggccgatcct gctttccgcg ctcgtgcgca aggtttagcg
     8041 gcggtggact ctggtatggc gggtaaagcc attcccgaac tacaacaggc ggtgcgggcg
     8101 aacccgaaag acagtgaagc tctgggggcg ctgggccagg cgtattctca gaaaggcgat
     8161 cgcgccaatg cagtggcgaa tctggaaaaa gccctcgcac tggacccgca cagcagcaac
     8221 aacgacaaat ggaacagtct gctgaaagta aaccgctact ggctggcgat ccagcagggc
     8281 gatgctgcgc tgaaagccaa taatcctgac cgggcagaac gcctgttcca gcaggcgcgt
     8341 aatgtcgata acaccgacag ttatgcagtg ctggggctgg gcgatgtggc gatggcgcga
     8401 aaagattatc ccgccgccga acgttattat cagcagacct tgcgtatgga cagcggcaac
     8461 actaacgccg tgcgcgggct ggcaaatatt taccgccagc aatcgccaga aaaagctgaa
     8521 gcgtttatcg cctcgctctc tgccagtcag cggcgtagca ttgatgatat cgaacgcagc
     8581 ctgcaaaacg accgtctggc acagcaggca gaggcactgg aaaaccaggg caaatgggcg
     8641 caggcggcag cacttcagcg gcaacgactg gcgctggacc ccggcagcgt atggattact
     8701 taccgacttt cgcaggatct ctggcaggcc ggacaacgca gccaggccga tacgttaatg
     8761 cgcaatctgg cgcagcagaa gtcgaacgac ccagagcagg tttacgctta cgggctgtac
     8821 ctacgcttac gggctgtacc tctctggtca tgaccaggac agagcggcgc tggcgcatat
     8881 caacagcctg ccgcgtgcgc agtggaacag caatattcag gagctggtta atcgactgca
     8941 aagcgatcag gtgctggaaa ccgctaaccg cctgcgagaa agcggcaaag aggcagaagc
     9001 ggaagcgatg ctgcgccagc aaccaccttc cacgcgtatt gacctcacgc tggctgactg
     9061 ggcgcaacaa cgacgtgatt acaccgccgc ccgcgctgca tatcagaatg tcctgacgcg
     9121 ggagccagct aacgccgacg ccattcttgg tctgacggaa gtggatattg ctgccggtga
     9181 caaagcggcg gcacgtagcc agctggcgaa actgcccgct accgataacg cctcgctgaa
     9241 cacacagcgg cgcgtggcgc tggcacaggc gcagcttggc gataccgcag cagcgcagcg
     9301 gacgtttaat aagttgatcc cgcaggcaaa atctcagcca ccgtcgatgg aaagcgcgat
     9361 ggtgctgcgt gatggtgcga agtttgaagc gcaggcgggc gatccaacgc aggcgctgga
     9421 aacctacaaa gacgccatgg tcgcatccgg tgtgactacg acgcgtccgc aggataacga
     9481 cacctttacc cgactgaccc gtaacgacga gaaagatgac tggctgaaac gtggcgtgcg
     9541 cagcgatgcg gcggacctct atcgccagca ggatcttaac gtcacccttg agcacgatta
     9601 ctggggttcg agcggcaccg gtggttactc cgatctgaaa gcgcacacta ccatgttgca
     9661 ggtggatgcg ccgtattctg acgggcggat gttctttcgc agtgatttcg tcaatatgaa
     9721 cgtcggcagt ttctccacta atgccgatgg caaatgggat gacaactggg gcacctgtac
     9781 attacaggac tgtagcggca accgcagcca gtcggattcc ggtgccagcg tggcggtcgg
     9841 ctggcgaaat gacgtctgga gctgggatat cggtaccacg ccgatgggct tcaacgtggt
     9901 ggatgtggtc ggcggcatca gttacagcga tgatatcggg ccgctgggtt acaccgttaa
     9961 cgcccaccgt cggcccatct ccagttcttt gctggccttt ggtgggcaaa aagactcccc
    10021 gagcaatacc gggaaaaaat ggggtggcgt acgtgccgac ggtgtggggc taagtctgag
    10081 ctacgataaa ggtgaagcaa acggcgtctg ggcatcgctt agtggcgacc agttaaccgg
    10141 taaaaatgtc gaagataact ggcgcgtgcg ctggatgacg ggctattact ataaggtcat
    10201 taaccagaac aatcgccgcg tcacaatcgg cctgaacaac atgatctggc attacgacaa
    10261 agatctgagt ggctactcac tcggtcaggg cggttactac agtccgcagg aatacctgtc
    10321 gtttgccata ccggtgatgt ggcgggagcg cacggaaaac tggtcgtggg agctgggggc
    10381 gtctggctcg tggtcgcatt cacgcaccaa aaccatgccg cgttatccgc tgatgaatct
    10441 gatcccgacc gactggcagg aagaagctgc gcggcaatcc aacgatggcg gcagcagtca
    10501 gggcttcggc tacacggcgc gggcattact tgaacgacgt gttacttcca actggtttgt
    10561 tggcacggca attgatatcc agcaggcgaa agattacgca cccagccatt tcctgctcta
    10621 cgtacgttat tccgccgccg gatggcaggg tgacatggat ttaccgccgc agccgctgat
    10681 accttacgcc gactggtaag ttttcagata gcgcctctct taatgccgct gcgatcgggt
    10741 atactcgggc ggcaatctgg gatttccggg gggagacaat ttgcgcgtaa gtcgctcgtt
    10801 aacaatcaag cagatggcaa tggtggcagc cgttgtcctg gtgttcgttt ttattttttg
    10861 caccgttttg ctgttccatc tggtccagca gaatcgctat aacacggcta cgcaactgga
    10921 aagcattgct cgctctgtcc gcgaaccctt atcttcagct attttgaaag gcgatattcc
    10981 cgaagcggaa gctattcttg ccagcattaa accggcaggc gtggtcagcc gtgccgatgt
    11041 agtgctgcct aaccagttcc aggcgctgcg taaaagtttt attccagagc gcccggtgcc
    11101 gataatggtt actcgcctgt ttgagctacc ggttcaaatc tcgctgggcg tttactcgct
    11161 cgaacgtccg gcaaacccgc agccaattgc ctatctggta ctacaggcgg attccttccg
    11221 tatgtataag ttcgtgatga gcaccctctc aacgttagtg accatttact tacttttgtc
    11281 gcttatcctg accgtcgcca tcagctggtg cattaaccgc ctgattttgc atccgttacg
    11341 caatattgct cgcgaactta acgccatccc agcccaggag cttgttggtc accaactggc
    11401 attaccgcgt ctgcatcagg acgatgaaat cggtatgttg gtgcgcagtt acaacctcaa
    11461 ccagcaattg ctgcagcgcc attatgaaga acagaacgaa aatgcgatgc gcttcccggt
    11521 gtcggatttg ccgaacaaag ccttgctgat ggagatgctg gagcaggttg tcgcgcgtaa
    11581 acaaaccacc gcgctgatga tcatcacctg tgaaaccctg cgtgatactg cgggcgtgct
    11641 gaaagaggcg caacgagaaa ttctgctgct gacgctggtg gaaaaactca aatcggtact
    11701 gtcgccacgt atgatcctcg cgcagattag cggttatgac tttgctgtca ttgccaacgg
    11761 tgtacaggaa ccgtggcacg caatcacctt aggtcagcaa gtgctcacta tcatgagcga
    11821 gcgcctgccg attgaacgta ttcaactccg tccgcactgt agcattggcg tggcgatgtt
    11881 ctacggcgat ctcaccgccg aacagcttta cagtcgcgct atttctgcgg catttaccgc
    11941 tcgccataaa ggcaagaatc agattcagtt ctttgatccg cagcagatgg aagccgccca
    12001 gaagcggttg acggaagaga gcgatatcct taatgcactg gaaaatcatc agtttgctat
    12061 ttggttacag ccacaggtcg agatgaccag cggtaaactg gtcagtgcgg aagtgttact
    12121 gcgtatccag caaccggatg gcagttggga cctgccggat ggcttaatcg atcgcattga
    12181 gtgctgtggg ctgatggtta ccgtcggtca ctgggtgctg gaagagtcct gtcgattgct
    12241 tgcagcctgg caagagcgcg gcattatgct gcccttgtcg gtaaacctct ctgcgctgca
    12301 actgatgcac ccaaatatgg tggcggatat gctggaactg ttaacccgct atcgcattca
    12361 gccgggaaca ctgattctgg aagtgacaga aagccgacgt attgacgacc ctcatgctgc
    12421 ggtggcaatc ctccgtccgc tgcgcaatgc cggagttcgg gtggcgctgg atgatttcgg
    12481 catgggctac gcagggctgc gtcagctgca gcatatgaaa tcgttgccaa tcgacgtact
    12541 gaaaatcgac aaaatgtttg ttgaaggctt gccggaagat agcagcatga ttgctgcaat
    12601 tatcatgctg gcgcagagcc tgaacttaca aatgattgcc gaaggcgtgg agactgaagc
    12661 acaacgcgac tggctggcaa aagcgggcgt tggtattgcc cagggcttcc tttttgctca
    12721 cccactccct attgaaatct tcgaagagag ttacctggaa gaaaagtagc taccccaaac
    12781 tgattacaaa actttaaaaa gtgctggttt gtgcgagcca gctcaaactt tttaaccttt
    12841 ttgtttcaat tatgatccag gtacatttct gtgatgttgt ctgggtgtta ttttaaggcc
    12901 gcaggtaccc cataacctta caagacctgt ggttttacta aaggacaccc tatgaaaacc
    12961 tctctgttta aaagccttta ctttcaggtc ctgacagcga tagccattgg tattctcctt
    13021 ggccatttct atcctgaaat aggcgagcaa atgaaaccgc ttggcgacgg cttcgttaag
    13081 ctcattaaga tgatcatcgc tcctgtcatc ttttgtaccg tcgtaacggg cattgcgggc
    13141 atggaaagca tgaaggcggt cggtcgtacc ggcgcagtcg cactgcttta ctttgaaatt
    13201 gtcagtacca tcgcgctgat tattggtctt atcatcgtta acgtcgtgca gcctggtgcc
    13261 ggaatgaacg tcgatccggc aacgcttgat gcgaaagcgg tagcggttta cgccgatcag
    13321 gcgaaagacc agggcattgt cgccttcatt atggatgtca tcccggcgag cgtcattggc
    13381 gcatttgcca gcggtaacat tctgcaggtg ctgctgtttg ccgtactgtt tggttttgcg
    13441 ctccaccgtc tgggcagcaa aggccaactg atttttaacg tcatcgaaag tttctcgcag
    13501 gtcatcttcg gcatcatcaa tatgatcatg cgtctggcac ctattggtgc gttcggggca
    13561 atggcgttta ccatcggtaa atacggcgtc ggcacactgg tgcaactggg gcagctgatt
    13621 atctgtttct atattacctg tatcctgttt gtggtgctag tattgggttc aatcgctaaa
    13681 gcgactggtt tcagtatctt caaatttatc cgctacatcc gtgaagaact gctgattgta
    13741 ctggggactt catcttccga gtcggcgctg ccgcgtatgc tcgacaagat ggagaaactc
    13801 ggctgccgca aatcggtggt ggggctggtc atcccgacag gctactcgtt taaccttgat
    13861 ggcacatcga tatacctgac aatggcggcg gtgtttatcg cccaggccac taacagtcag
    13921 atggatatcg tccaccaaat cacgctgtta atcgtgttgc tgctttcttc taaaggggcg
    13981 gcaggggtaa cgggtagtgg ctttatcgtg ctggcggcga cgctctctgc ggtgggccat
    14041 ttgccggtag cgggtctggc gctgatcctc ggtatcgacc gctttatgtc agaagctcgt
    14101 gcgctgacta acctggtcgg taacggcgta gcgaccattg tcgttgctaa gtgggtgaaa
    14161 gaactggacc acaaaaaact ggacgatgtg ctgaataatc gtgcgccgga tggcaaaacg
    14221 cacgaattat cctcttaatc tcaccactta tgcccgcact cccttccccg agtgcgggtg
    14281 tttgcgcata attgacccct gtgcctgaac ttaccacctg ggtgagttca ggtttatttc
    14341 acttcttccg tgacattttc atgttcttgc ggtctaacac gaagtgtttt taacgtcatt
    14401 tagactgact gacaacgggc agttttttat taccaggata gttgatcagg ggttcacatg
    14461 cagggcacaa aaattcgact tttagcgggc ggtttgctga tgatggccac tgctggctat
    14521 gtgcaggcag atgcgctcca gcctgatcca gcatggcaac aggggacgct ttccaacggt
    14581 ttacagtggc aagtgctgac caccccccag cgtcccagcg atcgtgttga aattcgcctg
    14641 ctggttaata tcggttcgct cgccgaaagt acacaacaga gcggttacag tcacgccatc
    14701 cctcgtattg cgctaacgca aagcggtggc cttgacgcag cacaggcgcg ttcattgtgg
    14761 cagcagggga tcgaccctaa acgcccgatg ccgccggtaa ttgtctctta tgacaccacg
    14821 ctgtttaatc tgagtttgcc caataaccgt aacgatttgc tgaaagaagc gctctcttat
    14881 ctggcaaatg ccactggcaa attgaccatc acaccagaaa ccatcaacca cgcgctgcaa
    14941 agtcaggaca tggtggcaac ctggcctgcc gatactaaag agggctggtg gcgctatcgt
    15001 ctgaaagggt caaccttgtt aggtcacgat cctgccgatc cgctgaaaca acccgttgaa
    15061 gcggaaaaaa ttaaagattt ctatcagaaa tggtacaccc cggatgcaat gacgctactg
    15121 gtggtgggaa acgtggatgc gcgctcggtt gtcgaccaaa tcaacaaaac gtttggcgaa
    15181 ctgaaaggca aacgtgaaac gccagctccg gtgccgacgc tttctccgct gcgtgcggaa
    15241 gcggtgagta ttatgactga cgcggtgcgt caggaccggt tatctatcat gtgggatacg
    15301 ccgtggcagc cgattcgtga atcagccgca ctgctgcgct actggcgtgc ggacctggcc
    15361 cgcgaggcgc tgttctggca tgttcagcaa gcgttaagcg ccagtaacag caaagacatc
    15421 ggtcttggat ttgactgccg tgtgctgtat ctgcgtgcgc agtgtgccat caacatcgaa
    15481 tcaccaaacg acaagctgaa cagcaacctt aatctggtgg cgcgtgaact ggcgaaggtt
    15541 cgcgataaag gtctgccgga agaagagttc aatgcgttag tggcgcaaaa gaaactggag
    15601 ctgcagaaac tgtttgccgc ctatgcgcgg gctgataccg atattctgat gggtcagcgg
    15661 atgcgttcgc tgcaaaatca ggtcgtcgat atcgcgccgg agcagtatca gaaactgcgg
    15721 caggatttcc ttaacagcct gacggtggag atgttaaatc aggatctgcg tcagcagttg
    15781 tcgaatgata tggcgttaat actgctgcag ccgaaaggcg agccggaatt taacatgaaa
    15841 gcgttgcagg cggcctggga tcaaatcatg gccccatcga ctgctgctgc cgccacctct
    15901 gtcgccacgg atgacgtaca tcctgaagtg acggatattc cacctgcaca gtaatgcctg
    15961 atggtgatga gttagcacaa ccatcaggta tttatggatg agctggatag aggggtaaca
    16021 ctttatccag ccttttgcat atgctgcgtt tacgctggca tcgcctcacg cgggataatc
    16081 gcgccgcgat actgaataac ggtacttgcg gtcaggtgcc cgcgtttcgc cgcgttttcc
    16141 gcactgccgc ccgtcaaaca taccgccaga taaccggcgc tgaaagagtc gccagctgcg
    16201 gtggtatcga tcactttttc tttcggcagt ttcaccgccg gaacatccac taacccttcg
    16261 ccagcaatgg acaccaggca agaatccgcc ccgcgtttca ccaccacttc tttcacgccc
    16321 gcgttatggg tgcgcgcaat gacgtcttcc accggctgtt gaccccacag cgcgtcttca
    16381 tcgtccagcg tcaggaaggc gatatccgtg cattccagca tttgttggta cacctgctgt
    16441 gtctcttctt tgctggccca caggcgcgga cgatagttat tgtcgaaaat cacttttccg
    16501 ccgttggcgc ggcattcgcg cagcagggaa agcagctttt cgcggctggt cgggcttaag
    16561 atcgccaggc taatcccgct caggtagaga taatcgaaat tcgccagctc ttcgcaaatc
    16621 gccgcagact gctcactctc cagccagaat ttggcggcgg cttcgttccg ccagtagtag
    16681 aacgtacgct cgccggtgct gtcggtttca atgtagtaaa ggcccggcag acggttttcc
    16741 atccgttggg tcagggaagt atcaacgttc tcgccgtgcc aggcgtccag catttgctgg
    16801 ctaaaactgt ccgttcccag cgccgttacg taatgaacgg ttaatgccgc aggatcgacc
    16861 tgacgggcga tatagacgga agtgttcagg gtatcgccgc cgaaaccgcg cttaacgtcc
    16921 gcgcctttct cggaaagctc aatcatgcat tcgccaatca cggcaatctt tttggacata
    16981 gtcgtgaacc tgatctgtaa aaaattagcg ttagtttgcg ctgtggtggt ttgctggtca
    17041 accatattaa aacagtgttc cattattttt ttgagccaga acgtattcct gaaagattcc
    17101 gttgtggagt gaggaaattt accccggcca ggcggtaaag ttctgccctt gcgcgccgat
    17161 aatctttgtc gagtccgggc agcatcactt ttaaacacag gacatctttg atgataaggc
    17221 aggttatcca gcgaataagc aaccctgaag caagcatcga gagcttgcag gaacggcgtt
    17281 tttggttgca gtgtgagcgt gcttacacct ggcagccgat ctatcaaaca tgcggacggt
    17341 taatggccgt ggagctatta acggtggtca cgcatccctt gaacccttcg caacgcctgc
    17401 cgccggatcg ctattttact gaaatcaccg tcagccatcg gatggaggtt gtgaaagagc
    17461 agattgattt gctggcgcaa aaagccgact tctttgtaga gcacgacctg ctggcatcgg
    17521 tcaatattga tggccctacg ctcatcgccc tgcgtcagca accaaaaatc ctgcgccaaa
    17581 ttgagcgtct tccctggctg cgtttcgaac tggtggaaca catccgcctg ccgaaagatt
    17641 caacctttgc ctcgatgtgt gaatttggcc cgctgtggct ggatgatttt ggcaccggga
    17701 tggcaaattt ctctgcgcta agtgaagtgc gttatgacta catcaaaatc gcgcgagagc
    17761 tgttcgtgat gctgcgtcag tcgccggaag gccgcacact cttttctcag cttttacatc
    17821 taatgaatcg ctattgtcgc ggggtgattg tcgagggcgt agaaacaccg gaagagtggc
    17881 gtgatgttca gaactcgccc gcattcgccg cacaaggctg gtttctttca cgcccggcac
    17941 cgatggaaac gctgaatacg gcggttctgg cgctataagc tgcctcattt tccgctgggc
    18001 tggactatct ttaggactgg cacaggaaga atatgaggaa gcgaacgatg agcaaggcag
    18061 gcaaaataac cgctgcgatt tcaggggctt tcttgttgtt gattgtcgtg gcgatcattt
    18121 tgattgcaac ttttgactgg aatcgactca aaccgaccat caaccagaaa gtctctgcgg
    18181 agttgaatcg tccgttcgct atccgtggcg atctgggcgt ggtgtgggag cggcaaaaac
    18241 aagaaactgg ctggcgcagc tgggtgccgt ggcctcatgt acatgcagaa gacatcattc
    18301 ttggtaatcc accggatatt cccgaagtca cgatggtgca tttgccgcga gtagaggcaa
    18361 cgctggcacc gctggcgctg ctgaccaaaa cggtctggct gccgtggatc aagctcgaaa
    18421 agcccgacgc gcgcctgatt cgcctctccg aaaagaacaa taactggacg tttaatcttg
    18481 ccaacgatga caacaaagac gcgaatgcaa agccgtcggc atggtcgttt cggctggata
    18541 atattctttt cgatcaaggg cggatcgcca ttgatgacaa agtaagcaaa gcggatctgg
    18601 aaatttttgt tgatccgtta ggcaagccgc tgccgttcag cgaagttact ggatcgaaag
    18661 gtaaagcgga taaagaaaag gtgggcgatt acgtttttgg cctgaaggcg cagggacgtt
    18721 ataacggtga accgctcacg ggtacgggaa aaataggcgg tatgctggcg ctgcgtggcg
    18781 aagggacgcc gtttccggta caggctgatt tccgttcagg taatacccgt gttgcttttg
    18841 atggcgtcgt gaatgaccca atgaagatgg gcggtgtcga tttacggctt aaattttctg
    18901 gcgattcact gggtgatctc tatgaactga cgggcgttct gctgcccgat accccgccgt
    18961 ttgaaacgga tggtcggctg gtagcgaaaa tcgacactga aaaatcgtcg gtctttgatt
    19021 atcgcggttt taatgggcga attggcgata gcgatatcca cggttctctg gtctacacca
    19081 ccggaaagcc acgaccaaaa ctggaaggtg atgtcgagtc gcggcaattg cggctggcgg
    19141 acctgggacc gttgattggc gttgattccg ggaaaggggc agaaaagtcg aaacggtctg
    19201 aacagaagaa gggcgaaaaa agcgttcagc ctgcgggcaa agtgctgcct tatgaccgct
    19261 tcgaaaccga taaatgggac gttatggatg ccgatgttcg cttcaaaggg cggcgcattg
    19321 agcatggcag tagcctgccg attagcgatc tttctactca tatcatcctc aaaaatgctg
    19381 acctgcgcct gcaaccgctg aaatttggca tggcgggcgg cagcattgcg gcgaatattc
    19441 atctggaagg cgataaaaag ccgatgcagg ggcgggcaga tattcaggct cgtcgactga
    19501 aactgaaaga actgatgccc gatgtggaac tgatgcagaa gacgctgggg gaaatgaacg
    19561 gtgacgcgga actacgcggt agcggtaact cggtggcggc gcttttaggc aacagtaacg
    19621 gcaacctgaa actgttgatg aatgacgggc tggtgagccg caacctgatg gagattgttg
    19681 ggctgaatgt cggcaactac attgtcggtg cgatatttgg tgacgatgag gtgcgggtga
    19741 actgcgcggc ggcgaatctg aatattgcca acggcgtggc gcgcccgcag atttttgctt
    19801 tcgatactga gaacgcgttg attaatgtta ccggcacggc aagttttgct tcagaacagc
    19861 tggatttgac tattgatccg gagagtaaag ggattcggat tatcacactg cgttcgccgc
    19921 tgtatgtgcg tgggacgttt aaaaatccgc aggctggggt gaaagccgga ccgctgattg
    19981 cccgtggtgc tgttgctgcg gcactggcaa cgctggtaac accgacggcg gcgttactgg
    20041 cactgatctc accttccgaa ggggaggcta atcagtgtcg gacgatattg tcgcagatga
    20101 agaagtgatg ataattgccg gatgtctgac atccggcaat taaacagatt tagatttttg
    20161 gcaaagtgtg ctttgttcat gccggatgcg gcgtgaacgc cttatccggc ctacaaatcc
    20221 ttgtgaattc aatatattgt atggaaattc agtcctgata agcgtagcgc atcaggcttt
    20281 tcacacttat tacaacgact gatgtcgcgt ctcatgggtc agcagcaggg cgattaacgt
    20341 caagccagcc atcgccgcca gatataaccc caccgcacct aacccgtagt tagtctgcaa
    20401 ccaggctgcg atatacggtg caacggaagc cccgagaatc gacgctacgt tgtaagagaa
    20461 tgatgctccg gtgtaacgca cttctgttgg gaacagctct ggtaacagcg cgcccatcgg
    20521 cccgaaggtc agccccatca gacttaaccc cagcagcagg aaggcaaaaa ccagaatcgg
    20581 gttgccagaa ccgagcagcg ggttaaaggc gaacagcgcg aacaggatga tcagcgtggt
    20641 gataattacc atgcttttac ggcgaccaaa ggcatcagcc agtaatccgg cgactggcac
    20701 catcacgcca aaaccaataa ctgccatcat cagcatccac aacacttcgt tacgcggcag
    20761 gccaagccca actggcgcgg cggcggtact gaaggtcata gagtagaccg tcatgatgta
    20821 aaacagcgta taggttgcca gcatgatgaa cgtacccagt acggttacgc gaacatgttt
    20881 ggtcagcagc gtacccagcg ggatcttcac ctgttttttc gctttagcga ctttctcaaa
    20941 caccggcgac tcatgcagcg acacgcgaac atacaggccg ataatgacca gtaccgccga
    21001 gaagataaat ggcacacgcc agccccagct cataaactgc tcgtcggtca gtagccagga
    21061 aagcagcaag aaagtgccgt tggcaaagaa gaagccaatc ggtgcgccca gctgtgggaa
    21121 ggagccatac agtgcacgtt tgcgcggtgg ggcgttttca gtcgccagca gcgccgcgcc
    21181 gccccattca ccgcctaagc ccagaccctg accaaatcga gccagcgcca gcagcagcgg
    21241 ggcgaaaata ccaatcgtgg catagcccgg cagcagacca atcaccacgg ttgaaatccc
    21301 catcgttagc aacgaggcga ccagcgtcgc tttacgccca acgcgatcgc caaaatgacc
    21361 aaaaacggca gagccaatgg ggcgcgcgac gaaggcgatg gcgaaggtgg cgagcgactg
    21421 tagcgttgct gccgttggat cgccctgcgg gaagaagata tgcggaaaca caataacggc
    21481 cgcagtggcg taaatgtaaa agtcgaagaa ctcaatggcc gtgccaatga gagaggcgac
    21541 aaggacttta ttacgcgagt tgatcggcgt gtattcttgc tcgtggtcga gtgttgtggc
    21601 tgttgcttgc ataatctttt cttatttttt ggctaacgaa tagccatatt acgcacagca
    21661 aaagtgatat ttcaatgtgt aacaaatcgc ggaaatggtc aaaaaccagg caaaaagggg
    21721 ataatgttta ggccagtgat ttttcttcta tgaaatgctt cacataattt tataaatagt
    21781 ggataaaact ggtttcaggt taaataaaag ttaccaactg gatttatatg ctggggttat
    21841 gagttggaat gaaattgttt gctcccaaat agatggctga agaggctctg gggaagagtt
    21901 tcaagaggtt atgtgcgcat tatagccgga tgcgatgctg gcgcatctta tccggcctac
    21961 ggggagccag gccgaataaa atcgtatgcc ggataaggcg tttacgccgc atccggccta
    22021 ttttaaggct gcgttttccc tggcattcgc gggtcgtctt tatattcggc ggtggcaatc
    22081 cacgccgcgc aaaacagcgt caaacgggcg aagaagtaga aaaatgccat cagccccagc
    22141 acggaaccaa atgctgcgcc agagggggat ttcatcagcg atggcagggt gtaggtcatc
    22201 acgattttaa tcacttcaaa accaatagca gccagaaatg ttccgcgaat cagggctttt
    22261 ttgcgcggac ggtggcgcgg taaccgccag aagatccaga agaaaagcag atagttggcg
    22321 aagatggaaa tcgccaggcc aatcaaccgc cacgtcggtt tcagccactc aatgctgttc
    22381 aggtgcaggg cgctaataat catttgctgc gccgaaccgg caaccgaggt gatcgaaagc
    22441 gtcacaatca gcgcaatcaa caaaccaatc agcgaaataa aatcacgtag atattttacc
    22501 cagaacttct cctgatcttg cggcgagcgt tcccagacat cgcgcgactg ggcgcgaatc
    22561 gcttcacgca ggttacccat ccagttgatg ccggaataaa gcgccacagc caggccgaca
    22621 agccctacag tcgtacgctg ctgaacggcg gtgttgatgg tgtttttcaa cgtggcggct
    22681 agcgtcggat cgctgatgtt ttgcagaatt ttgtcgaaga tatcctgtag caacatcgga
    22741 tgggaggcca gcacaaagcc ccctgcggca aacgacacca tcaaaatcgg gatcatcgac
    22801 aaaaatgaga aataggtgat agccgcacca aactggttac ccagccgatc gttaaagcgt
    22861 tctgtcgcgc gaatcaggtg cgcaataacc ggttggcgtt ggactttttc ggcagtggtg
    22921 gtgacggttt ccagcgcctg gctaactttg ccgcctttct cgctattatc caactgctta
    22981 atcggctcgt gctccagatc ctgggtggga cgtttgatct cgttttcctg cgtcatccgc
    23041 tcttttgtcc ttctgttgtg gtcattctta aaaagtatag tcggtcagtc caggtactct
    23101 ttcattacgc cagccagcca ctgcataaac aggtttacac gccgggaaag ctcccgacgc
    23161 tgcggataaa ccagcgaaag ggagagcggc tcggcacggt agcctggcaa cacttcaata
    23221 agctgcccgg cacgcagggc ttcgcgcacg gcaatgcgcg ggatctgaat aatccccaga
    23281 ccggcaagac cggcggcgag atagttttcg ctactgttta ccgtcaacat gccgccagac
    23341 ttaaaccact ggacgccatt aacgctggca acctcaaaac ctaacggatg tacacccagg
    23401 tgcggtgtat aacgcaccat tgcgtgtgaa gtcagatcat cggggctttg cggataacca
    23461 aagcgcgtca gatagtgcgg actggcacag ttgaccatgg tcagtttgcc gaggggacgg
    23521 gcgataacgc catcttccgg taacgcgcca gtgcgtatca cgcaatcaaa accatcatga
    23581 agaatatcta ccggacggtt atggctactc agttccagct caattcccgg atgctgatag
    23641 agaaattccg acagacgcgg cagtaacagg tttttcgcga ttcctgacgg gatgtcgatg
    23701 cgtaatttac cgctgatact ggtggcatcc tgttgaaaca gaccgtccag ttcgctgaga
    23761 ttactcaaca catcttttgc ccgttgataa taggtcatgc cttctggcgt gagtttgacc
    23821 cgtcgcgtag tgcgctggag aagctgggtg ccaagttgat gttccagtgc ctgtatctgg
    23881 cgcgaaacac ttccctttgg caaagcaaag aaatccgccg cgcgggaaaa actttccagc
    23941 tccgcgactt tgatgaacaa ctgcattgcg tgaattttat ccatttcttt gaccgattgt
    24001 tgttcacagc gaaacagtaa cgcgcatatc gcattgttta ttgttttttc taacatctaa
    24061 tgccgggttt cacaatgttg tcatgacgaa tgtggcgatt tctcagcagg aaaagggcgg
    24121 aatagcttta tgagaaaggt gaatcccctt aaacagagcg tatttttcag tgattccctt
    24181 taaagtgaat agaggacgta ttggctattt gtgcttattt ttgtctcatt gccatttgtt
    24241 attttggctt gtagtcgcgg aatttcattt ttaattgcat gcgtactaac caccaatata
    24301 aggaaatcct caaaattgtt ttaaatagtg ccctcgacat gtaatttcag gcgtttagct
    24361 gtaactattt atatcggtaa ctattttcta cgcgtcatct ctgtagaatg gccaccgcca
    24421 aatggcgaat tcttattttt tctcgcgggt aatacccttc cactacttaa gtctttttaa
    24481 cgtattcagg tggagattca gttatgcaaa tagtcatgtt tgacaggcag tcaatattta
    24541 ttcatggaat gaaaatcagt ttacagcagc gtattccagg agtgagtatt cagggggcca
    24601 gtcaggcaga cgagttatgg caaaagctgg aaagttaccc tgaagcctta gttatgctcg
    24661 atggcgatca ggatggtgag ttttgctact ggttgctgca aaaaaccgtg gtgcagtttc
    24721 ctgaggttaa ggtgttaatc acggcgacgg attgcaataa acggtggtta caggaagtta
    24781 ttcattttaa tgtgctggcc attgtgcctc gtgattcaac cgtcgagaca tttgcgttgg
    24841 cggtaaatag tgcggcgatg gggatgatgt ttctgcctgg agactggcgg acgacgccgg
    24901 aaaaggacat taaagatctc aaatcgttaa gcgcccgcca gcgggagatt ttaaccatgt
    24961 tagcggcggg cgaatcaaat aaggagatcg gcagagcgct gaatatcagt accggaacgg
    25021 taaaagcgca tctggaatcc ctgtatcgcc gtctggaagt gaaaaatcgc acccaggcgg
    25081 caatgatgtt aaatatctcc tcctg
//
LOCUS       DAZPLI010000049        25075 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZPLI010000049 DAZPLI010000000
VERSION     DAZPLI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25075
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.049"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..807
                     /locus_tag="ACHUU3_001921"
     CDS             <1..807
                     /locus_tag="ACHUU3_001921"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR2082000.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICAGRPDEGIPAVCFKLKEGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            963..2498
                     /gene="gadC"
                     /locus_tag="ACHUU3_001922"
     CDS             963..2498
                     /gene="gadC"
                     /locus_tag="ACHUU3_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR2082001.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2629..3948
                     /locus_tag="ACHUU3_001923"
     CDS             2629..3948
                     /locus_tag="ACHUU3_001923"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR2082002.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4320..5702
                     /gene="dosC"
                     /locus_tag="ACHUU3_001924"
     CDS             4320..5702
                     /gene="dosC"
                     /locus_tag="ACHUU3_001924"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFR2082003.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5727..8126
                     /gene="dosP"
                     /locus_tag="ACHUU3_001925"
                     /gene_synonym="pdeO"
     CDS             5727..8126
                     /gene="dosP"
                     /locus_tag="ACHUU3_001925"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFR2082004.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            8385..8966
                     /gene="ddpX"
                     /locus_tag="ACHUU3_001926"
     CDS             8385..8966
                     /gene="ddpX"
                     /locus_tag="ACHUU3_001926"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFR2082005.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8980..10530
                     /locus_tag="ACHUU3_001927"
     CDS             8980..10530
                     /locus_tag="ACHUU3_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR2082006.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10532..11554
                     /locus_tag="ACHUU3_001928"
     CDS             10532..11554
                     /locus_tag="ACHUU3_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR2082007.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11551..12446
                     /gene="ddpC"
                     /locus_tag="ACHUU3_001929"
                     /pseudo
     CDS             11551..12446
                     /gene="ddpC"
                     /locus_tag="ACHUU3_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
     gene            12443..13429
                     /locus_tag="ACHUU3_001930"
     CDS             12443..13429
                     /locus_tag="ACHUU3_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR2082008.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            13458..14354
                     /locus_tag="ACHUU3_001931"
     CDS             13458..14354
                     /locus_tag="ACHUU3_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFR2082009.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIAIARALSSQPDVIVLDEPT
                     SALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQ
                     QVLTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHG
                     CEVRQSLAIREDGRELRCWRAL"
     gene            complement(14410..14841)
                     /gene="osmC"
                     /locus_tag="ACHUU3_001932"
     CDS             complement(14410..14841)
                     /gene="osmC"
                     /locus_tag="ACHUU3_001932"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR2082010.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            15186..15401
                     /gene="bdm"
                     /locus_tag="ACHUU3_001933"
     CDS             15186..15401
                     /gene="bdm"
                     /locus_tag="ACHUU3_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFR2082011.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEQGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15503..15640
                     /gene="sra"
                     /locus_tag="ACHUU3_001934"
     CDS             15503..15640
                     /gene="sra"
                     /locus_tag="ACHUU3_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFR2082012.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15797..17494
                     /gene="maeA"
                     /locus_tag="ACHUU3_001935"
     CDS             15797..17494
                     /gene="maeA"
                     /locus_tag="ACHUU3_001935"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR2082013.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17628..18630
                     /gene="adhP"
                     /locus_tag="ACHUU3_001936"
                     /pseudo
     CDS             17628..18630
                     /gene="adhP"
                     /locus_tag="ACHUU3_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            18776..19060
                     /locus_tag="ACHUU3_001937"
     CDS             18776..19060
                     /locus_tag="ACHUU3_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR2082014.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(19288..19941)
                     /gene="fdnI"
                     /locus_tag="ACHUU3_001938"
     CDS             complement(19288..19941)
                     /gene="fdnI"
                     /locus_tag="ACHUU3_001938"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR2082015.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19934..20818)
                     /gene="fdnH"
                     /locus_tag="ACHUU3_001939"
     CDS             complement(19934..20818)
                     /gene="fdnH"
                     /locus_tag="ACHUU3_001939"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR2082016.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20831..23878)
                     /gene="fdnG"
                     /locus_tag="ACHUU3_001940"
     CDS             complement(20831..23878)
                     /gene="fdnG"
                     /locus_tag="ACHUU3_001940"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(23291..23293),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR2082017.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            24110..24991
                     /gene="yddG"
                     /locus_tag="ACHUU3_001941"
     CDS             24110..24991
                     /gene="yddG"
                     /locus_tag="ACHUU3_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR2082018.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         6129 a         6380 c         6358 g         6208 t
ORIGIN      
        1 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgccgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca caaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg tcgtgaaggc
      421 tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga tgagatcgcc
      481 aaactggggc cgtatgagtt catctgtgcc ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagaggg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg
      721 gaagactaca aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc
      781 cagcagaaca gctttaaaca tacctgataa cgtttaacgg taacggtgtc ccgaaacgaa
      841 cccgtttcgg gataatttcc aaagtctgtt cactggcatt agcaacggaa aatattgttc
      901 tgaatacgct tcagaacaaa acaggtgcgg ttccgacagg aataccgttt tagggggata
      961 atatggctac atcagtacag acaggtaaag ctaagcagct cacattactc ggcttttttg
     1021 ccataacggc atcgatggta atggctgttt atgaataccc taccttcgca acatcgggct
     1081 tttcattagt cttcttcctg ctattaggcg ggattttatg gtttattccc gtgggacttt
     1141 gtgctgcgga aatggccacc gtcgacggct gggaagaagg tggtgtcttc gcctgggtat
     1201 caaatactct ggggccgaga tggggatttg cagcgatctc atttggctat ctgcaaatcg
     1261 ccattggttt tattccgatg ctctatttcg tgttaggggc actctcctac atcctgaaat
     1321 ggccagcgct gaatgaagac cccattacca aaactattgc agcactcatc attctttggg
     1381 cgctggcatt aacgcagttt ggtggcacga aatacacggc acgaattgct aaagttggct
     1441 tcttcgccgg tatcctgtta cctgcattta ttttgatcgc attagcggct atttatctgc
     1501 actccggtgc ccccgttgct atcgaaatgg attcaaagac cttcttccct gacttctcta
     1561 aagtgggcac cctggttgta tttgttgcct tcattttgag ttatatgggc gtagaagcat
     1621 ccgcaaccca cgtcaatgaa atgagcaacc cagggcgcga ctatccgttg gctatgttac
     1681 tgctgatggt agcggcaatc tgcttaagct ctgttggtgg tttgtctatt gcgatggtca
     1741 ttccgggtaa tgaaatcaac ctctccgcag gggtaatgca aacctttacc gttctgatgt
     1801 cccatgtggc accggaaatt gagtggacgg ttcgcgtgat ctccgcactg ctgttgctgg
     1861 gtgttctggc ggaaatcgcc tcctggattg ttggtccttc tcgcgggatg tatgtcacag
     1921 cgcagaaaaa cctgctgcca gcggcattcg ctaaaatgaa caaaaatggc gtaccggtaa
     1981 cgctggtcat ttcacagctg gtgattactt ctatcgcgtt gatcatcctc accaataccg
     2041 gtggcggtaa caacatgtcc ttcctgatcg cactggcgct gacggtggtg atttatctgt
     2101 gtgcttattt catgctgttt attggctaca ttgtgttggt tcttaaacat cctgacttaa
     2161 aacgcacatt taatatccct ggtggtaaag gggtgaaact ggtcgtggct attgtcggtc
     2221 tgctgacttc aattatggcg tttattgttt ccttcctgcc gccggataac atccagggtg
     2281 actctaccga tatgtatgtt gaattactgg tcgttagttt cctggtggta cttgccctgc
     2341 cctttattct ctatgctgtt catgatcgta aaggcaaagc gaataccggc gtcactctgg
     2401 agccaatcaa cagtcagaac gcaccaaaag gtcacttctt cctgcacccg cgtgcacgtt
     2461 caccacacta tattgtgatg aatgacaaga aacactaacc atgtctgaat ggttataaga
     2521 caagggagcg ataattcatc gctccctttt tcgtgctcgc tgtcgtattg acttaaccgg
     2581 gataagtacg agaatgagcg cacatctgtt taccggaaac cagcatatat ggatatctgc
     2641 tcccgaaacg agaaattagc gattagaaga ccagcgatac tggttgcact ggcactttta
     2701 ctgtgtagtt gtaaaagcac gcctccagag tccatggtga caccaccagc aggttcaaag
     2761 ccaccagcca cgacgcaaca atcgtcacaa ccgatgcgtg gcatctggct ggccacggtg
     2821 tctcgcctcg actggccacc ggtttcctcg gttaacatta gtaaccccac cagccgggcc
     2881 cgtgtacaac aacaggcgat gatcgacaaa ctggatcatc tgcaacgtct cggtataaac
     2941 acggtctttt tccaggttaa gccggacggt accgccctgt ggccatcgaa aattttgcca
     3001 tggtccgatc ttatgaccgg taagattggt gaaaatccgg gttacgatcc gctgcaattt
     3061 atgctcgacg aagcccacaa gcgtgggatg aaagtacacg cctggtttaa cccctatcgc
     3121 gtatcggtta atacgaagcc cggtactatc agggaactga atagcactct gtctcaacaa
     3181 ccggcgagcg tctatgtgca acaccgtgac tggatcagaa cgtctggcga tcgctttgtc
     3241 ctcgacccgg gcatccctga ggttcaggac tggatcacat caatagtcgc agaagtggtt
     3301 tcccgctatc cggtagatgg cgtgcagttt gacgactatt tctatactga gtcaccgggt
     3361 tcacggctaa atgataacga aacgtaccgc aaatacggag gcgcatttgc gtcaaaagca
     3421 gactggcggc gcaacaatac tcagcagtta attgcaaaag tatcgcacac cattaaaagc
     3481 attaagccgg gagtcgaatt tggcgttagc ccggcaggcg tgtggcgtaa ccgatcacac
     3541 gatccgctcg gttccgatac ccgaggcgcg gcagcctatg acgaatccta cgctgacacc
     3601 cgtcgatggg tggaacaagg attgctggat tacattgctc cccaaattta ctggccgttc
     3661 tcacggagtg ccgcgcgtta tgacgtgttg gcaaaatggt gggcggatgt cgttaaaccg
     3721 accaggaccc gcctgtatat cggtatcgcc ttctataaag tgggtgaacc ttcaaagata
     3781 gagccagact ggatgattaa cggcggcgta ccggaactga aaaagcagct cgatcttaac
     3841 gatgcggtac cagaaattag cggcaccatc ttgttccgtg aggactatct gaataaaccg
     3901 cagactcaac aagcggtcag ctatctgcaa agtcgttggg gcagttaaac tcccatgctt
     3961 actttgtaac aagccggtgc tttacccacc ggcttgttac accttgtgaa atatcccact
     4021 agtaatcatg cttacataag tcaaattaac cacgcaatta accacactta gttaaacagc
     4081 tataaatccc taataaaaat gtatgaatat gaaaaaccat acgatattcg ctgtcaccga
     4141 aaaataatta cgatgggcta tactcttctg aagaacagta gcgtagattg aaactgtcct
     4201 aagtcgctta tttcttttca gtatatcttc atatttcagg agaatatggt gcagttagtc
     4261 aatctcgcac gttgcgtttt gattccgtct gatttcccag ccttataagg gtgtgaatta
     4321 tggagatgta ttttaaaaga atgaaagatg agtggaccgg acttgtcgaa caggcagatc
     4381 cactcattcg tgctaaagcc gcggaaattg ccgttgcgca tgctcattat ctgagtatcg
     4441 agttttatcg aattgtccgc atcgacccgc atgccgaaga attcttgagt aatgaacaag
     4501 ttgagcggca gttgaaaagt gcgatggaac gctggattat taacgtgctt tctgcccagg
     4561 ttgacgatgt cgaaaggcta atacaaatcc agcataccgt cgcggaagtg catgcccgca
     4621 taggaattcc ggtagaaatt gtcgagatgg ggtttcgggt gctgaaaaaa atcctctatc
     4681 cggtaatctt ctcttcggat tattccgccg cagaaaaact tcaggtctat catttctcga
     4741 ttaacagtat tgatatcgcg atggaagtga tgacccgcgc gtttaccttt agtgacagta
     4801 gtgcctcaaa ggaagatgaa aattatcgta tcttctcgtt actggaaaac gccgaagaag
     4861 aaaaagaacg gcaaatagcc tcaatacttt catgggaaat agatattatc tataaaatcc
     4921 tgctggattc tgatttaggc agtagtttgc cattaagcca ggctgatttt ggcctgtggt
     4981 ttaaccataa aggtcgacat tattttagtg gcattgccga agtaggccat atctcccgtc
     5041 tcattcagga tttcgacggt attttcaatc aaaccatgcg taacaccagg aatttgaata
     5101 acagaagtct gcgggtgaaa tttttattac agataagaaa taccgtatcg cagattatta
     5161 ccttgctgcg tgaattgttt gaagaagtat cgcgccacga agtcggtatg gatgtactaa
     5221 cgaaattact taaccgccgt ttcctgccga ctatcttcaa acgcgaaatt gcccatgcca
     5281 accggaccgg tacaccgctg tcagtgctga ttattgacgt tgataaattc aaagagatca
     5341 acgatacgtg gggccataac actggcgatg aaattctgcg taaagtctct caggcctttt
     5401 atgacaacgt ccacagtagt gattatgttt ttcgctacgg gggcgatgaa tttatcattg
     5461 ttttgactga agcttctgaa aacgaaacgt tacgtaccgc agaacgtatt cgcagtcggg
     5521 tggagaaaac caaactgaaa gccgcaaacg gcgaggatat tgccctctca ctttccatcg
     5581 gtgccgccat gtttaatggt catcctgact atgagcgcct cattcaaata gccgatgaag
     5641 ctctgtatat cgccaaaaga cgaggtagaa accgtgttga actctggaaa gccagtcttt
     5701 agatgcgcca ggatgcagag gtaatcatga agctaaccga tgcggaaaat gccgccgatg
     5761 gcattttttt ccccgccctt gaacaaaata tgatgggcgc ggtgttaatt aacgaaaatg
     5821 atgaagtgat gtttttcaac cccgccgcag agaagctctg ggggtacaaa cgtgaagaag
     5881 tcattggcaa taacattgat atgctgattc cgcgggattt gcgtcctgcg catcctgaat
     5941 acattcgtca taaccgtgaa ggtggtaaag cgcgcgtcga ggggatgagt cgggagctgc
     6001 agctggagaa aaaagacggc agtaaaatct ggacccgttt tgcgctatcg aaagtgagcg
     6061 ccgaggggaa agtttattac ctggcgctgg tacgggatgc cagcgtagaa atggcgcaaa
     6121 aagaacagac ccgacaattg attattgccg ttgaccatct cgaccgaccg gtgattgtcc
     6181 tcgatccgga acgccatatt gtgcagtgca atcgcgcatt taccgaaatg tttggttact
     6241 gcattagcga agccagcggt atgcagcccg atacactcct gaacattcct gaattccctg
     6301 ccgataaccg cattcgttta caacagttgc tatggaaaac cgcccgcgat caggacgaat
     6361 ttctgctgtt gacacgcacc ggtgaaaaaa tctggattaa agcctctatc agcccggttt
     6421 atgacgtgct cgcgcatctg cagaacctgg taatgacctt ctcggatatc accgaagaac
     6481 ggcagatccg ccagcttgaa ggcaatattc tcgccgccat gtgcagcagc ccgccatttc
     6541 atgaaatggg ggaaatcatt tgtcgtaaca tcgaatctgt tctcaacgaa tcgcatgttt
     6601 cgctgttcgc actgcgcaac gggatgccga tacactgggc gtcatcttcg cacggtgcag
     6661 aagttcaaaa tgcgcaaagc tggtcagcga ccattcgtca gcgtgatggt gcgcctgcgg
     6721 ggatcctgca aattaaaacc tcgtcaggag cagaaaccag cgcctttatc gaacgcgtgg
     6781 cagatatcag ccagcatatg gccgcgctgg cgctggaaca ggaaaaaagc cgtcagcata
     6841 ttgaacaact catccaattt gatccgatga ccggtctgcc aaatcgcaat aacctgcaca
     6901 attacctcga tgaccttgtc gacaaagccg tctctccggt ggtgtatctc atcggtgttg
     6961 accatattca ggatgtgatt gatagccttg gctatgcgtg ggccgatcag gcattgctgg
     7021 aagtggtcaa tcgctttcgt gaaaaactca aaccggatca gtatctctgt cgtatcgaag
     7081 gtacgcagtt tgtcctcgtg agcctggaaa acgacgtcag taacattacc caaatcgccg
     7141 atgagctacg gaatgtggtc agcaagccga taatgattga cgataaacca ttcccgctaa
     7201 ccttgagtat tggcatcagc tacgacgtgg gtaaaaaccg cgattacctg ctctccactg
     7261 ctcacaatgc aatggattat attcgcaaga atggcggtaa cggctggcag ttcttcagcc
     7321 cggcgatgaa cgaaatggta aaagagcgtt tggttttagg cgcagcgctg aaagaagcga
     7381 ttagcaataa ccaactgaaa ctggtttacc agccgcaaat cttcgcagaa acgggtgaac
     7441 tgtacggcat cgaagccctt gctcgctggc acgatcccca gcatggtcat gtaccccctt
     7501 cacggtttat tcctctcgca gaagagattg gtgaaatcga aaatattggg cgctgggtca
     7561 tcgcggaagc ttgccgtcag ttagcagaat ggcgtagcca gaatattcat atcccggcgt
     7621 tatccgtgaa cttgtcggcg ctgcactttc gcagtaatca gctgcctaat caggtgtctg
     7681 atgcaatgca agcctggggt attgacggcc accagctgac ggtagaaatc acggaaagca
     7741 tgatgatgga acacgatacc gaaatcttta agcgcattca gatcctgcgc gatatgggcg
     7801 tgggcttatc ggtagatgat tttggcacgg gcttttccgg attatcccgc ttagtcagtc
     7861 ttccggtaac ggaaatcaaa attgacaaaa gttttgtcga tcgttgtctg acggaaaaac
     7921 gcatccttgc cttacttgaa gccattacca gcattgggca aagcctcaat ttaaccgtcg
     7981 tggcggaagg cgtcgaaacc aaagagcaat ttgagatgct acgcaagatc cactgtcgcg
     8041 ttattcaggg atatttcttt tcccgccccc tacccgccga ggaaattcca ggctggatga
     8101 gcagcgtgtt accgctgaaa atctgacaaa ttcctctcgc ccgcactcgc gggttttcat
     8161 ttaacgtgac actgtcactt aaatactgtg atttcagcta cgattccggg agattccttc
     8221 cttaacttcc cctcttgcac caaaactgca ctacgctgca caaatgttga tcaaaagtta
     8281 aaaaatataa ctttaagcca gcattctggc gcttatcccg gcatggcatg agatctgcat
     8341 aagcggaaag cgcagcaatt tttgtcttaa tacaggaacc gtttatgtcg gataccaccg
     8401 aactggttga tttagccgtg atcttccctg atctggagat cgaattgaaa tacgcctgcg
     8461 ctgataacat cacaggtaaa gctatttacc agcaagcgcg ttgtctgtta cacaaggatg
     8521 cgattaccgc gctggcgaaa agtatcagta tcgcccagct gtcaggctta caactggtga
     8581 tttacgatgc gtatcgccca caacaagcac aggcgatgtt gtggcaagcc tgcccagacc
     8641 cgcaatatgt tgttgatgtg acggtcggtt ctaatcacag ccgtggcacg gcgatcgacc
     8701 tgacgcttcg tgatgagcac gggaacatcc tggatatggg cgcagggttc gatgaaatgc
     8761 acgagcgctc ccatgcctat cacccttccg tcccgcccgc cgctcagcgc aatcgcctgt
     8821 tgctgaatgc gataatgact ggtggcggtt ttgtcggtat ctccagcgaa tggtggcact
     8881 tcgaattacc ccaggcagcg agttaccctc tgctcgctga tcaattctct tgttttatat
     8941 cccctggcac acagcacgtc agttaatcag gaaactgtca tgaagagatc gatatcgttt
     9001 cgtcccacat tgctcgcgct cgtccttgcc acaactttcc cggttgcgca cgccgccgta
     9061 ccgaaagata tgctggtgat tggtaaggcc gccgatccac aaaccctcga cccggcggta
     9121 acaatagata ataacgactg gacagtgacc tacccgtctt atcagcggct ggttcagtac
     9181 aaaacggacg gtgataaagg ctcaaccgac gttgaaggcg atctggcaag tagctggaaa
     9241 gcgtctgacg atcaaaaaga gtggacgttc accctgaagg acaatgctaa atttgccgat
     9301 ggcacacctg tcactgccga agcagtaaaa ctctcttttg agcggttact aaaaatcggc
     9361 caggggccag cagaagcatt tcccaaagat ttaaagattg atactcccga cgaacatacg
     9421 gtgaagttta cccttagcca accattcgca ccgttcctct acacgctagc gaatgacggt
     9481 gcatccatta tcaatccggc ggtcttaaag gaacatgcag cggatgatgc tcgcggcttc
     9541 ctcgcgcaaa ataccgccgg ttccggacca tttatgctga aaagctggca aaaaggtcag
     9601 caattagttc tggtgccaaa tccgcattac cccggcaata aaccgaactt taagcgagta
     9661 tcggtaaaaa ttatcggtga aagtgcctcc cgtcgcctgc agctctcccg tggcgacatt
     9721 gacattgccg atgcgctgcc ggtggatcaa ctcaacgccc tgaagcagga aaataaagtc
     9781 aatgtggcag agtatccgtc actgcgcgtt acctatctgt atctgaataa cagcaaagcg
     9841 cctcttaatc aggcggatct gcgtcgggcc atttcctggt ctaccgatta tcagggcatg
     9901 gttaacggca ttctgagtgg taacggaaaa cagatgcgcg gcccgattcc ggaaggcatg
     9961 tggggctacg atgcgacggc aatgcaatac aaccatgacg aaacgaaagc caaagccgaa
    10021 tgggataaag tgacgagcaa acccaccagc ctgacgtttc tctactccga taacgatccg
    10081 aactgggagc ctattgctct ggcgacacaa tccagtctca acaagctggg catcaatgtg
    10141 aagctggaaa agctggcgaa cgccaccatg cgcgacagag tgggtaaagg tgattacgac
    10201 attgcgattg gcaactggag tccggatttt gccgacccgt atatgtttat gaattactgg
    10261 tttgagtcag acaaaaaagg tctgccgggt aaccgctcgt tctatgaaaa cagtgaggtc
    10321 gataagttac tgcgcaatgc gcttgcgacc accgaccaga cgcagcgtac ccgggactac
    10381 cagcaggcac agaaaatcgt cattgatgac gctgcttatg tgtacctgtt ccagaaaaac
    10441 taccaactgg cgatgaacaa agaggtgaaa ggctttgtgt tcaatcccat gctggaacag
    10501 gtcttcaata tcaataccat gagtaaataa cgtgaccttc tggagtattt tacgccaacg
    10561 ctgctggggg ctggtgctcg tggtggcggg cgtctgcgtg attacgttta ttatctcgca
    10621 tctgatccct ggcgatccgg cgcggttact ggcgggtgac cgcgccagcg atgctatcgt
    10681 ggaaaatatt cgccagcaac tgggactgga ccagccactg tatgtacagt tttaccgcta
    10741 cttcagcgac ctgtttcatg gtgacctggg aacatccatt cgtaccgggc gtccggtgct
    10801 ggaagagttg cgtatatttt tcccggcgac gctggaactg gctttttgtg ccctgctgct
    10861 ggcactcctg attggcatcc cgctgggcat actctctgca gtctggcgaa atcgctggct
    10921 ggatcatctg gtgcgaataa tggccattac cggaatctcc acacctgcgt tctggcttgg
    10981 actgggcgtc attgtgctgt tttatggtca tctgcaaatt cttcccggcg gcggaaggct
    11041 tgatgactgg ctggatccac caacgcacgt taccggcttt tatctgctcg atgcgctgct
    11101 tgaaggcaac ggtgaagtct tcttcaatgc gttgcaacat ctcatcttac cggcattaac
    11161 gctggcgttc gttcacctgg gaattgtcgc tcgccagatc cgctcagcga tgctggaaca
    11221 attgagtgaa gactacattc gtaccgcccg ggccagcggc ttgcccggct ggtatatcgt
    11281 tttatgttat gcgctaccca atgcgttgat cccatcgatt accgtattgg gtctggcgct
    11341 gggcgatttg ttgtatggcg cagtgctcac cgaaaccgtt tttgcctggc ccggaatggg
    11401 tgcatgggta gtaacatcaa tacaggcgct cgacttcccg gcagtgatgg gctttgccgt
    11461 cgtggtttca tttgcttatg tgctggtcaa cctggtggtg gatttgctct atttgtggat
    11521 tgatccgcgt atcggacgtg gaggtggtga atgatgctaa gcgaggaaac gtccgccgta
    11581 cgcccacaaa aacaaacgcg atttaacggt gcaaaactgg tatggatgct gaaaggcagt
    11641 ccgctcaccg tgaccggcgc agtcatcatt gtttaatgct attgatgatg attttttcac
    11701 cgtggcttgc gacgcatgat cccaacgcca ttgatttaac cgcccgcctt ttgccgcctt
    11761 ctgcggcgca ctggtttggc accgatgaag tgggacgcga tctgttcagc cgcgtactgg
    11821 tcggcagtca gcaatcaatt ctcgccggat tagtcgtggt tgccattgcg ggtatgattg
    11881 gttcgctact cggatgtcta tccggtgtgc ttggcggacg cgcagacgcc atcatcatgc
    11941 gcatcatgga cattatgctg tcgattcctt cgttggtact gacaatggca ctggctgccg
    12001 ctctcgggcc gagtttattt aacgccatgc tggcgattgc tattgtgcga attccctttt
    12061 atgtgcgcct ggcgcggggg caagcattag ttgtacgcca gtatacctat gttcaggcgg
    12121 cgaaaacctt tggtgcgtcg cgttggcatc tgatcagctg gcatatttta cgtaactccc
    12181 tgccgccgct gatcgtgcag gcatcgctgg atatcggtag cgcgatttta atggccgcca
    12241 cgttgggatt tattggcctg ggtgctcaac aaccgagtgc tgaatggggc gcgatggtag
    12301 ctaatggtcg caactatgta ctcgatcagt ggtggtattg cgcatttccg ggggcagcga
    12361 ttttgcttac cgccgtcggg tttaatctct ttggtgatgg tattcgcgat ctgcttgacc
    12421 cgaaagcagg aggaaagcag tcatgaccca acccgttctg gacattcaac agctgcattt
    12481 gagtttcccc ggttttaacg gttatgttca cgtgctcaac aatgtgtcct tgcagattaa
    12541 ccgcggtgaa attgtcggtc tggtgggaga atccggctca ggtaaatcag tcaccgcaat
    12601 gctgattatg cgtctgctac cgacgggcag ttattgcgta catcgggggc agatttcact
    12661 gctgggagac gatgttctta acgctcggga gaaacagctt cgtcagtggc gcggcgcacg
    12721 agtggcgatg atctttcagg aaccgatgac cgccctcaat ccgacacgtc gaataggtct
    12781 tcagatgatg gacgtgatcc gccatcatca accaataagt cgtcgggaag ccagagctaa
    12841 agcgattacc ctgctggaag agatgcaaat cccggatgcc gtggaagtta tgtcgcgcta
    12901 tccgtttgag ctttcaggtg gtatgcgcca gcgggtaatg attgcgctgg cattctcctg
    12961 cgagccgcaa ttgattattg ccgacgaacc gactacggcg ctggacgtca cggtacagtt
    13021 gcaggtactg cgtttgctta aacataaagc tcgcgccagt ggaactgcgg tactgttcat
    13081 cagccatgat atggccgtgg tgtcgcaact gtgcgatagc gtttacgtga tgtatgccgg
    13141 aagcgtaatt gaaagcggcg tgacggcaga cgttatccat catccccggc atccgtatac
    13201 cattggtttg ctgcaatgcg caccagaaca tggagtacca cgccagccat tacccgccat
    13261 tccagggacg gtaccaaacc tcacccattt gcctgacggc tgcgcttttc gcgatcgttg
    13321 ctatgcggca ggtgcacagt gtgaaaacgt cccggcgctg acagcgtgtg gtgacaacaa
    13381 ccagcgctgc gcctgttggt atccgcagca ggaggtcata agtatctgac acgttattaa
    13441 cgttacgcga cgtccatatc aatttcccgg cccgtaaaag ctggcttggt aaaactacgg
    13501 aacatgttca tgcgattaat ggtattgatt tacagatccg ccgtggtgaa accttaggga
    13561 tcgtcggtga gtcaggctgc ggcaaaagca ccctcgcaca gcttttaatg ggtatgctgc
    13621 aaccgagcca cgggcaatac atccgttcag gatcacaacg cattatgcag atggtgtttc
    13681 aggacccgct ctcttcgctt aatccgcgct tatcggtgtg gcgcatcatc acagaaccgc
    13741 tctggatagc taagcgtagt agtgaacaac agcggcgagc gttggcaaag gagttggctg
    13801 tgcaggtggg tattcgtccg gagtatctcg accgcctgcc tcatgcgttc tccggcgggc
    13861 agcggcaacg catcgccatt gccattgcca gagcactctc ttcgcagcct gacgtgattg
    13921 tgcttgatga gccaacctcg gcgctggata tctccgtgca ggcgcagatc ctcaatttac
    13981 tggtaacgct acaggaaaat cacgggctga cttatgtgct gatttcacac aatgtctcgg
    14041 tgatacgtca tatgagcgat cgggtggcgg tgatgtatct cgggcagatt gtagaactgg
    14101 gcgacgcgca gcaggtgctg acggcacctg cacatccata cacccgatta ttgctggatt
    14161 ccctccccgc cattgataaa ccgctggagg aagaatgggc attatgtaaa acggatctgc
    14221 cgggaaaccg cacgttgcca caaggctgtt ttttctacga acgttgccct ctggcaaccc
    14281 acggatgtga agtccggcaa tcattagcaa taagggagga cggacgtgag ctccggtgct
    14341 ggcgggcgct gtagagatgc taacaacgca tcaggcattc acgcgcctga cgcgtcatcc
    14401 ggcaatgctt tacgatttca actggtaatc cagcgtaatt tccgctttca gtacctgaga
    14461 aaccgggcat cctgcttttg ctttctggat gatgccgtca aaggtagagg catcaatacc
    14521 cggcaccgca acttcactct tcagtgcgat tttcgtaatc gcaaaatcgg catccacttt
    14581 atccagcgac acatcggcgg tggtatcaat cgatgttggc gtgaatcccg cttcccccag
    14641 cattaatgaa agcgccattg agaaacatgc ggcatgcgct gcgccaatca gttcttcagg
    14701 gttggttcct ttttcgcctt caaaacgcgt gttaaatcca tacggctgtt ggttcagcac
    14761 gccactctgg gtggatactg ttcccttccc gcgtttgata tcgccttccc agtgtgcctg
    14821 acctttctta tggattgtca ttgttgctct cctgtgggct taaaaaggaa attatagact
    14881 acgtgagaaa cagcacgagg ataagcagga tattccgaat aaaatacggc gaaaatatta
    14941 cttctgcact taaattaaat tcccacaata gataaccggg aatatatatc tcaagagata
    15001 ttgcatcaat atcaatcaac tcaccacaat aataatacat atcaatatca tggagttaaa
    15061 atataaaaat catcaaccag gactaatctt aacaacgaag cggcaaatat ttgccatgct
    15121 gaatgtgctt tataaatctg cgatccgtag cagacaccat aaatacacag acacggagaa
    15181 tcactatgtt tacttattat caggcagaaa attcaacagc agaacccgct ctggttaatg
    15241 ccattgagca aggccttcgg gcagagcaag gtgttgtcac tgaagatgac attctcatgg
    15301 agctgaccaa atgggtggaa gcttctgata acgacatcct cagtgatatc taccagcaaa
    15361 cgatcaatta tgtggtcagt ggccagcacc ctacgcttta aggtgctatg cttgatcggc
    15421 aacctaattt aggggtttag cacgtgtttc ttcgctacgg cgatgttgtc cttaaaacta
    15481 gcaacaggat tgaggagtta aaatgaaatc gaaccgtcag gcacgtcata ttcttggact
    15541 ggaccataaa atttctaacc agcgcaaaat agttaccgaa ggtgacaaat ccagcgtggt
    15601 aaataaccca accggcagaa aacgccccgc tgaaaagtaa ttcataacca tcagtcctca
    15661 atgacgatta aacaccattg cctgcgcaat ggtgtttttg tttttatctg ctttatactt
    15721 ggggccgacg ccctggcggt aaagcaaaga cgataaaagc gtgccaggga tggatattca
    15781 aaaaagagtg agtgacatgg aaccaaaaac aaaaaaacag cgttcgcttt atatccctta
    15841 cgctggccct gtactgctgg aatttccgtt gttgaataaa ggcagtgcct tcagcatgga
    15901 agaacgccgt aacttcaacc tgctggggtt actgccggaa gtggtcgaaa ccatcgaaga
    15961 acaagcggaa cgagcatgga tccagtatca gggattcaaa accgaaatcg acaaacacat
    16021 ctacctgcgt aacatccagg acaccaacga aaccctcttc taccgtctgg taaacaatca
    16081 tcttgatgag atgatgcctg ttatttatac cccaaccgtc ggcgcagcct gtgagcgttt
    16141 ttctgagatc taccgccgtt cacgcggcgt gtttatctct taccagaacc gccacaatat
    16201 ggacgatatt ctgcaaaacg taccgaacca taatatcaaa gtgattgtgg tgactgacgg
    16261 tgaacgtatt ctggggcttg gtgaccaggg catcggcggg atgggcattc cgatcggtaa
    16321 actgtcgctc tataccgcct gtggcggcat cagcccggcg tatacccttc cggtggtgct
    16381 ggatgtcgga acgaacaacc aacaactgct taacgatccg ctgtatatgg gctggcgtaa
    16441 tccgcgtatc actgacgacg aatactatga attcgttgat gaatttatcc aggctgtgaa
    16501 acaacgctgg ccagacgtgc tgttgcagtt tgaagacttt gctcaaaaaa atgcgatgcc
    16561 gttacttaac cgctatcgca atgaaatttg ttcttttaac gatgacattc agggcactgc
    16621 ggcggtaaca gtcggcacac tgatcgcagc aagccgagcg gcaggtggtc agttaagcga
    16681 gaaaaaaatc gtcttccttg gcgcaggttc agcgggatgc ggcattgccg aaatgatcat
    16741 cgcccagacc cagcgcgaag gattaagcga ggaagcggcg cggcagaaag tctttatggt
    16801 cgatcgcttt ggcctgctga cggacaagat gccgaacctg ctgcctttcc agaccaaact
    16861 ggtgcagaaa cgcgaaaacc tcagtgactg ggataccgac agcgatgtgc tgtcactgct
    16921 ggatgtggtg cgcaatgtaa aaccagatat tctgattggc gtctcaggac agaccgggct
    16981 gtttacggaa gagatcatcc gtgagatgca taaacactgt ccgcgtccga tcgtgatgcc
    17041 gctgtctaac ccgacgtcac gcgtggaagc cacaccgcag gacattatcg cctggaccga
    17101 aggtaacgcg ctggtcgcca cgggcagccc gtttaatcca gtggtatgga aagataaaat
    17161 ctaccctatc gcccagtgta acaacgcctt tattttcccg ggcatcggcc tgggggttat
    17221 tgcttccggc gcgtcacgta tcaccgatga gatgctgatg tcggcaagtg aaacgcttgc
    17281 acagtattcg ccgctggtgc tgaacggcga aggtctggta ctaccggaac taaaagatat
    17341 tcagaaagtc tcccgtgcaa ttgcgtttgc ggttggcaaa atggcgcagc agcaaggcgt
    17401 ggcggtgaaa acctctgccg aagctttgca acaagccatt gacgataatt tctggcaagc
    17461 cgaataccgc gactaccgcc gtacctccat ctaagcctgc gcccggtagt gaaggctacc
    17521 gggcaatttt cctctccctt tttcagatct catccatact gggtagtggc gaataaatct
    17581 catttgcctc acctgctatg cagaacatca tccgaaaagg aggaactatg aaggctgcag
    17641 ttgttacgaa ggatcatcat gttgacgtta cggataaaac actgcgctca ctgaaacatg
    17701 gcgaagccct gctgaaaatg gagtgttgtg gtgtatgtca taccgatctt catgttaaga
    17761 atggcgattt tggtgacaaa accgacgtaa ttctgggcca tgaaggcatc ggtgtggtgg
    17821 cagaagtggg tccaggtgtc acctcattaa aaccaggcga tcgtgccagc gtggcgtggt
    17881 tctacgaagg atgcggtcat tgcgaatact gtaacagtgg taacgaaacg ctctgccgtt
    17941 cagttaaaaa tgccggatac agcgttgatg gcggaagagt gcatcgtggt cgccgattac
    18001 gcggtaaaag tgccagatgg tctggactcg gcggcggcca gcagcattac ctgtgcgggg
    18061 gtcaccacct acaaagccgt taagctgtca aaaattcgtc ccgggcagtg gattgctatc
    18121 tacggtcttg gcggtctggg taacctcgcc ctgcaatacg caaagaatgt ctttaacgcc
    18181 aaagtgatcg ccattgatgt caatgatgag cagttaaaac tggcaaccga aatgggtgca
    18241 gatttagcga ttaactcacg caccgaagac gccgccaaaa ttgtgcagga gaaaaccggt
    18301 ggcgctcact ctgcggtggt aacagcagta gctaaagctg cgtttaactc ggcagttgat
    18361 gctgtccgtg caggcggtcg tgttgtggct gtcggtctgc cgccggagtc tatgagcctg
    18421 gatatcccac gtcttgtgct ggatggcatt gaagtggtcg gttcgctggt cggcacgcgc
    18481 caggatctaa ccgaagcctt ccagtttgcc gccgaaggca aagtggtgcc gaaagtcgcc
    18541 ctgcgtccgt tagcggacat caacaccatc tttaccgaga tggaagaagg caaaatccgt
    18601 ggccgtatgg tgattgattt ccgtcactaa gaggtctttg ctgcgactcc catgttcggg
    18661 tcgcagcatc gcacactctc caacatgaaa tggctaaatg gattattgtt gcgtaggaag
    18721 gaacaataat gatagtgttc ccagagaagg aacgctactg agagtaaaga cactaatgaa
    18781 aatggccaat catccccgcc cgggggacat tattcaggaa tcactggacg aacttaatgt
    18841 cagcctgcgc gagtttgcca gagcaatgga aattgcgccc tcaacggcaa gccgattgct
    18901 gaccggaaaa gcagctttga cgccagaaat ggcaattaaa ctttccgtgg tgatcggcag
    18961 ttcgccgcaa atgtggctga atctgcaaaa cgcctggagt ctggcagagg cagaaaaaac
    19021 ggtggatgtg tcgcgattac gccgtctggt tacgcaataa aaaatggcac cttataaagc
    19081 gccatttgaa actgatgaca aacgcaacat tgcctgatgc gctacgctta tcaggcctac
    19141 acgttcactt caatctattg tatttgaagg gttttcatgc cggataaggc attcacgacg
    19201 catccgggat gaacaaagcc cacgttgtct acaatatgaa aatggcgcct tttacagcgc
    19261 cattttcagc ttaaagttca aaagagatta tatcccttct tcactctctt ttttcgcttc
    19321 tgccttctcg atttcacgat accagcgcgg atggtgtttc ttcgcccagc gacggctcac
    19381 cttcccttcg atcatccctt taatcgaccc tttcacccag aatgccatat acatatggat
    19441 caggatggcg tggatcagga tgatacccgc agccgcgtgg atcagcaggc tgtagcgaac
    19501 aacctgcatc gggaagtact gcgcaaagta cggacgccag ataatcaccc cggtcaccag
    19561 cagcacgaaa atcatgctca tgatcgacca gaacatcatc ttttgcccgg cgttgtactt
    19621 accaacatcc gccactttgt gctcattgcc ttttaatact tcgacaatgt tcaacagcca
    19681 cggaatatct ttcttatccg ggatgttgtg atgcacaaaa cgcacaaaca taaacatcag
    19741 tgcgacgaaa atcgcaatgc cgaagaacgg gtgcaaaatg cgtcccatct gcggcgtacc
    19801 gaaggtttgc gtcagccatt gcagcgtcgg gaagaagaac gaaatcccgg acagcgccac
    19861 caggaagaag caaatcacca cggtccagtg acaggcgcga tcaataaatt tggtgcgcac
    19921 aatcattttc gacttactca tgatgatcct cctcgtcatc gtccacttcc ttattcgggc
    19981 caataccgat gtagtggaaa atcaacccgg caaaagtggc aataaagcca gccgctgcca
    20041 gcggtttcaa cgcgcctttc cacaggctta ccgaggtgtc gatcttcgga tctttcggca
    20101 gaccgtaata cagctccggc tgatcggcgt gatgcagcac gtacataacg tgcgtaccac
    20161 cgaccccttt cgggttgtag acgccagcat gttcgtaacc acgcgctttc agtttcgcca
    20221 cgcgctcttc cgccagctcc agcatctcct tcttggtgcc gaagtggata gccccggtcg
    20281 gacaggtttt cacacaagcc ggttcctggc cgacgctgac gcgatcgacg cagagcgtgc
    20341 atttatatac ccggttatcc tctttgttga ggcgcggaat attaaacgga cacccggcaa
    20401 tgcagtaacc acagccgatg cagttttccg actggaaatc gacaatcccg ttagcgtact
    20461 gaatgattgc accagcagac gggcacgcct tcaggcagcc gggatcttca cagtgcatac
    20521 agccgtcttt acggatcagc cactccagct tgccgttctg ttcggtttcg ctaaagcgca
    20581 tcaccgtcca ggacttggcg ctcagatcgg cggggttatc gtaaaccccg acgcagtgcc
    20641 ccacttcatc acggatgtcg ttccactccg aacacgccac ctgacaggct ttacagccga
    20701 tacaggtgga aacgtcgata agttttgcga cttctgcttt gtaatcacgc acctgagaag
    20761 gcggcgtgat ggagttagtt gcggaccttt tgataatatc ctgcgtttcc atagccatct
    20821 gttcgccccc ttacgccttc tcgatgttga ctaagaacgc tttatattcc ggcgtttgcg
    20881 agtttgcatc accgacattc ggcgtcagag tgttagcgat ataaccttta cgcgcgacac
    20941 cctcaaagcc ccagtggatt ggaataccca ccgtttcaac ctgctgaccg tttacattca
    21001 gcggtttcag acggcgcgtt accaccgcca ccgcgcggat aaagccacgc ttgcttgaga
    21061 cagtgacacg atcgccatta ttaatgcctt tcgccgccgc cagcgtttcg ctgatttcca
    21121 caaactgttc cggctgagca attgcgttga gcaatgcgtg cttggtccag gtgtggaagt
    21181 gctcggtcag acgataggtc gtacccacat acgggaactg ctctttttta cccatccgca
    21241 gcgcgtcttg ttcatacaga cgaacaaccg ggttagacac cacgttcgga tgcagcgggt
    21301 tagtgcccag cggcgtttca atcggctcgt agtgttccgg gaacggacct tccgccattt
    21361 tgttgatggc aaacaggcgt cccatccctt ccggctgcat gataaacggc ccggttggcg
    21421 tacccggtgc ggcattgccg aagtccggaa tatcgttacc cgtccacttg ctgccgttcc
    21481 actggatcag catccgtttc ggatcccacg gtttaccatt gatatccgcc gaagcacggt
    21541 tgtagagcac gcgacggttg agcggccacg cccaggccca tcccagcgta ttccccagac
    21601 cggatgggtc ggagttatcg cggttagcca tctggttgcc ctgctctgtc cagctaccgg
    21661 tgtagatcca gcaagaagat gcggttgtac cgtcatcacg cagatgcgca aagctactca
    21721 gcaactgacc tttcttcgca atcagcacgc cattagcgtc atagagatct tccagcgcat
    21781 agccgttatt ctctttcgcc acttcgtcag attgcggttc gtgcggctgc ttgtagttcc
    21841 agctcatctt catcagcggt tctacgcctt taccaccttc ggcctggtac agctcgcgca
    21901 gatgatggta gatacccgcc agaatttcgc cgtcgttacg cgcttcgccc ggcgcgtcct
    21961 gacctttcca gtgccactgc aaccagcgac cggagttagc gatagaacca tcttcttcgg
    22021 caaagcaggt cgaaggcaga cggaatactt cagtctgaat agacgccgga tcgacatcgt
    22081 tcgactcgcc gtggttctgc cagaaggtag aggtttcagt caccagcgga tcgataacca
    22141 ccatgtactt cagcttgctc aggcagctca ccactttgtt tttgtccggg aaggacgcaa
    22201 ccgggttaaa gccctggcag aagtaaccgg tgactttgcc ttcgtccatc atgttgaaat
    22261 acttgatgac gtcgtaggtc tggtcccact tcggcagcca gtcatagccc cagttgttct
    22321 ctttctgcgc ggcatcgcca tagaaagatt tcatcaggct aacgaagaac ttcggatagt
    22381 tgctccagta gttcacctga tcagccagcg tcgctttcgg cgtgttcgct tccagatacg
    22441 actgcaaatc aacctgtttt tctgacggaa gcgtcagata acctggcagg ctggtagaga
    22501 gcagacctaa gtcagtcagc ccctgaatgt tggagtgacc acgcaatgcg ttcacgccgc
    22561 caccggccat acccatgtta ccgagcagca actggatcat cgccatagta cggatgttct
    22621 gcgcacccac ggtgtgctgc gtccagccca gcgcgtacag gaaggtggtt gtgcgatccg
    22681 gtgcgctggt ggaggccagc acttcacaca ctttcaggaa gtcggctttt ggcgtaccgc
    22741 agatgttttc aacgacgtcc ggcgtgtagc gggaaacgtg cgctttcagc aggttccaca
    22801 cacagcgcgg atgagtcagt gtttcatcgc gttttgcata gccgttttca tcgaactgat
    22861 agttccagga cgacttatca tactggcgtt tttcagcgtc gtagccgctg aacaggccgt
    22921 cttcgaaagc aaaatcatca cgcaccagca ggctggcgtt ggtgtaatgt ttaacgtatt
    22981 cggcgttgat tttgttgttt tcgatcaggt agcgcaaaac gccagacagg aacgtaatgt
    23041 ccgtaccgga acgaataggt gcgtaaatat ccgccacaga agcggtacgc gtaaaacgag
    23101 gatcgacaac gatcaaggtt gcatcgttgt tgtttttcgc ttccatcgcc cagcggaaac
    23161 cgacgggatg cgcttcagca gcgttaccgc ccattaccat tacgacgtta gcgtttttga
    23221 tatccaccca gtggttggtc atcgcaccgc gaccaaatgt tggagcaaga cttgctaccg
    23281 ttggtccgtg tcagacgcgc gcctggttgt ctaccgccag catcccgagg gagcgggcaa
    23341 atttctgggt cagcatcccg gtttcgttgc tggcaccgga ggcacacagc ataccggtag
    23401 aaagccaacg gtttaccgtt acgccctgct cgttcttttc aataaagtta gcgtcacggt
    23461 cagctttcat cagcttcgca atacgggaga atgcttcttc ccagctaatg cgctgccatt
    23521 tgtcagaacc tggcgcacga tattccgggt agcgcagacg gttttcgctg ttgacgtaat
    23581 ccagcaaacc ggcccctttt gggcacagcg caccacggct taccggatga tccgggtcac
    23641 cttcaatgtg ataaatcgct tctctggcgt ttttcgcgcc atcacccagg ctatacatca
    23701 atagcccgca acctacggaa cagtatgtgc aggtgttacg gatctcttta gcgcgtaata
    23761 atttgtagtt tcgcgcctga gccagtgctt gcttcggggc aaagcccaat gccgctaccg
    23821 ttgttccagc cataccgccc gcgcagattt taaaaaattg tctgcgactg acgtccattg
    23881 ctttcctctt ttttcagggg tattactgcg cgaggaaact aacagtaaaa ttctgaatct
    23941 ttttgcgcga aatcaaaaag aacacgtgta tgccacaaaa taatccgcct gcgcaaatta
    24001 acctacctca atagcggtag aaaaacgcac cactgcctga caggccagtt aaaaaaatgc
    24061 tataaaattc agcttaattt ttaacggcaa gagagacaaa acagcgagca tgacacgaca
    24121 aaaagcaacg ctcatagggc taatagcgat cgtcctgtgg agcacgatgg taggattgat
    24181 tcgcggtgtc agcgaggggc tcggtccggt cggcggcgca gctgctatct attcattaag
    24241 cgggctgctg ttaatcttca cggttggatt tccgcgtatt cggcagattc cgaaaggcta
    24301 tttactcgcc gggagtctgt tattcgtcag ctatgaaatc tgtctggcgc tttccttagg
    24361 gtatgcggcg acccgtcatc aggcgattga agtgggtatg gtgaactatc tgtggcccag
    24421 cctgacaatt ctctttgcca ttctgtttaa tggtcagaaa accaactggc tgattgtacc
    24481 aggattatta ttagccctgg tcggcgtctg ctgggtgtta ggcggtgaga atgggttaca
    24541 ttatgatgaa atcatcaata atatcaccac cagcccattg agttatttcc tggcgtttat
    24601 tggtgcgttt atctgggcag cctattgcac agtaacgaat aaatacgcac gcggatttaa
    24661 cggaattacc gtttttgtcc tgctaacggg agcatgtctg tggatttact attttctcac
    24721 gccacaacca gaaatggtat ttagcacgcc cgtcatgatt aaactcatct ctgcggcatt
    24781 taccttagga tttgcttatg ctgcatggaa tgtcggtata ttgcatggca atgtcaccat
    24841 aatggcggta ggctcgtatt ttacgcctgt actttcctca gcgcttgcag ccgtgctgct
    24901 cagcgccccg ctgtcgttct cgttctggca aggcgcgcta atggtctgcg gcggttccct
    24961 gctctgctgg ctggcgacac gtcgtggtta aaataattgc cgggtctacg gcccggcagt
    25021 taatcagata aattccgaca atattaaaaa ataaatttca aataccagta attca
//
LOCUS       DAZPLI010000050        24593 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZPLI010000050 DAZPLI010000000
VERSION     DAZPLI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.050"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(167..577)
                     /locus_tag="ACHUU3_001942"
     CDS             complement(167..577)
                     /locus_tag="ACHUU3_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR2082019.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
     gene            complement(635..868)
                     /locus_tag="ACHUU3_001943"
     CDS             complement(635..868)
                     /locus_tag="ACHUU3_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFR2082020.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            1257..1802
                     /locus_tag="ACHUU3_001944"
     CDS             1257..1802
                     /locus_tag="ACHUU3_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFR2082021.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1777..3702
                     /locus_tag="ACHUU3_001945"
     CDS             1777..3702
                     /locus_tag="ACHUU3_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR2082022.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3699..3905
                     /locus_tag="ACHUU3_001946"
     CDS             3699..3905
                     /locus_tag="ACHUU3_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFR2082023.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3902..5503
                     /locus_tag="ACHUU3_001947"
     CDS             3902..5503
                     /locus_tag="ACHUU3_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR2082024.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5484..6803
                     /locus_tag="ACHUU3_001948"
     CDS             5484..6803
                     /locus_tag="ACHUU3_001948"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR2082025.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6813..7145
                     /locus_tag="ACHUU3_001949"
     CDS             6813..7145
                     /locus_tag="ACHUU3_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR2082026.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            7201..8226
                     /locus_tag="ACHUU3_001950"
     CDS             7201..8226
                     /locus_tag="ACHUU3_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR2082027.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            8268..8666
                     /locus_tag="ACHUU3_001951"
     CDS             8268..8666
                     /locus_tag="ACHUU3_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR2082028.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8678..9031
                     /locus_tag="ACHUU3_001952"
     CDS             8678..9031
                     /locus_tag="ACHUU3_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR2082029.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            9043..9621
                     /locus_tag="ACHUU3_001953"
     CDS             9043..9621
                     /locus_tag="ACHUU3_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2082030.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9618..10013
                     /locus_tag="ACHUU3_001954"
     CDS             9618..10013
                     /locus_tag="ACHUU3_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR2082031.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            10021..10761
                     /locus_tag="ACHUU3_001955"
     CDS             10021..10761
                     /locus_tag="ACHUU3_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2082032.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10777..11199
                     /locus_tag="ACHUU3_001956"
     CDS             10777..11199
                     /locus_tag="ACHUU3_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR2082033.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            11181..11615
                     /locus_tag="ACHUU3_001957"
     CDS             11181..11615
                     /locus_tag="ACHUU3_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR2082034.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11608..14169
                     /locus_tag="ACHUU3_001958"
     CDS             11608..14169
                     /locus_tag="ACHUU3_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR2082035.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            14166..14495
                     /locus_tag="ACHUU3_001959"
     CDS             14166..14495
                     /locus_tag="ACHUU3_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2082036.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14495..15193
                     /locus_tag="ACHUU3_001960"
     CDS             14495..15193
                     /locus_tag="ACHUU3_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR2082037.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15199..15942
                     /locus_tag="ACHUU3_001961"
     CDS             15199..15942
                     /locus_tag="ACHUU3_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR2082038.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15939..16511
                     /locus_tag="ACHUU3_001962"
     CDS             15939..16511
                     /locus_tag="ACHUU3_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR2082039.1"
                     /translation="MKTGAEAIRAMATQLPAFRQKLSDGWYQVRIAGQDVSTSGLTAQ
                     LHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNIYFSSLDNMVAQGNVMPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16572..20069
                     /locus_tag="ACHUU3_001963"
     CDS             16572..20069
                     /locus_tag="ACHUU3_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFR2082040.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            20140..20656
                     /locus_tag="ACHUU3_001964"
                     /pseudo
     CDS             20140..20656
                     /locus_tag="ACHUU3_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            20721..23804
                     /locus_tag="ACHUU3_001965"
     CDS             20721..23804
                     /locus_tag="ACHUU3_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR2082041.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTLETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAANSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTAYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTSSAGAHQH
                     SQTGPRTNSGSQPTGMFPAGSTQVSGTNQVGISGSLTSGTSQWVGKSSSEGNHTHSLS
                     GTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            23804..24320
                     /locus_tag="ACHUU3_001966"
                     /pseudo
     CDS             23804..24320
                     /locus_tag="ACHUU3_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT         5852 a         6119 c         7455 g         5167 t
ORIGIN      
        1 cgtcgccgta ttcccgtatt aacagagacc gtagcccgac ggggaactcc ttctgcgcga
       61 gtgtgcggga ataatcaaaa acgatgcaca ccggggttac cgggtacaca tatttcatca
      121 tgccagcgag tccggttctg gcacggaaga aaccggacgt tatgatttag tgcggaaata
      181 tttgtgtagt gttctgtatg ttctcagtaa agagtaatga attatcaaag gtatagtaat
      241 accttttgtt ttcgtggata tttgtaatcc atctgaaaac ccctgctgta gcaagatttt
      301 tcctgtattc gtaaaatgat aactctcctg atttgaatcc ttttaaggtg gcttctataa
      361 ggcatttatt ttttgaaaat cttacattta caaccttacc ctgtcctttt attaaaaccg
      421 tattatcgtt ttcaagaaca agatgaatat tctctgtggc taaatagtaa atgtaatgtg
      481 agacattgtg acgttttagt tcagaataaa accagtgata gtttaaatta tttcgcactt
      541 tatcgaatat ttgtttaaaa atggcaacct gagccattgt agtaccttcc atgtgatatg
      601 agggggcgta gtctgcacga ttatctaaat tgcttcaatc tggtctaacc tgttttctga
      661 gcaattcagt aatgtcactc ttttctttgt ttgcttcagg cgaaactctt ttttctgagc
      721 acagtctccg gcggcaggct tcaatgaccc aggctgagaa attcccggac cctttttgaa
      781 caagagcgat gttaatttgt tcaatcattt ggttaggaaa gcggatgttg cgggttgttg
      841 ttctgcgggt tctgttcttc gttgacatga ggttgccctg tattcagtgt cgctgatttg
      901 tattgtctga agttgttttt acgttaagtt gatgcagatc aattaatacg atacctgcgt
      961 cataattgat tatttgacgt ggtttgatgg cgtagatgca cgttgtgaca tgtagatgat
     1021 aattattatc attttgcggg tcctttccgg cgatccgaca ggttacgggg cggaaggcgc
     1081 gcgggttttc gctatttatg acaattttcc ggtttaaggc gtttccgttc ttcttcgccg
     1141 taacctaatg tttttattta aaacaccccc tgaaaagaaa ggaaacgaca ggtgctgaaa
     1201 acgggctttt tggcctctgt cgtttccttt ctctgttttt gtccgtggaa tgaacaatgg
     1261 aagtcaacaa aaagcagctg gctgacattt tcggtgcgag tatccgtacc attcagaact
     1321 ggcaggaaca gggaatgccc gttctgcgag gcggtggcaa gggtaatgag gtgctttatg
     1381 actctgccgc cgttataaga tggtatgccg aaagggatgc tgaaattgag aacgaaaagc
     1441 tgcgccggga agttgaagaa ctgcggcagg ccagcgagac agatctccag ccagggacta
     1501 ttgagtacga acgccatcga cttacgcgtg cgcaggccga cgcacaggag ctgaaaaatg
     1561 ccagagactc cgctgaagtg gtggaaaccg cattctgtac tttcgtgctg tcgcggatcg
     1621 caggtgaaat tgccagtatt ctcgacggga tccccctgtc ggtgcagcgg cgttttccgg
     1681 aactggaaaa ccgacatgtt gatttcctga aacgggatat catcaaagcc atgaacaaag
     1741 cagccgcgct ggatgaactg ataccggggt tgctgagtga atatatcgaa cagtcaggtt
     1801 aacaggctgc ggcattttgt ccgcgccggg cttcgctcac tgttcaggcc ggagccacag
     1861 accgccgttg aatgggcgga tgctaattac tatctcccga aagaatccgc ataccaggaa
     1921 gggcgctggg aaacactgcc ctttcagcgg gccatcatga atgcgatggg cagcgactac
     1981 atccgtgagg tgaatgtggt gaagtctgcc cgtgtcggtt attccaaaat gctgctgggt
     2041 gtttatgcct actttataga gcataagcag cgcaacacac ttatctggtt gccgacggat
     2101 ggtgatgccg agaactttat gaaaacccac gttgagccga ccatccgcga tattccgtcg
     2161 ctgctggcgc tggctccgtg gtatggcaaa aagcaccggg ataacacgct cactatgaag
     2221 cgtttttcca atggtcgtgg cttctggtgc ctgggcggta aagcggcaaa aaactaccgt
     2281 gaaaagtcgg tggatgtggc gggttatgat gaacttgctg cctttgatga ggatattgaa
     2341 caggaaggct ctccgacgtt ccttggcgac aaacgtattg aaggctcggt ctggccaaag
     2401 tccatccgtg gctccacgcc caaagtgaga ggcacctgcc agattgagcg tgcagccagt
     2461 gaatccccgc attttatgcg ttttcatgtt gcctgcccgc actgcgggga ggagcagtat
     2521 cttaaatttg gcgacaaaga gacgccgttt ggcctcaaat ggacgccgga tgacccctcc
     2581 agcgtgtttt atctctgcga gcataatgcc tgcgtcatcc gccagcagga gctggacttt
     2641 actgatgccc gttatatctg cgaaaagacc gggatctgga cccgtgatgg cattctctgg
     2701 ttttcgtcat ccggtgaaga gattgagcca cctgacagtg tgacctttca catctggaca
     2761 gcgtacagcc cgttcaccac ctgggtgcag attgtcaaag actggatgaa aacgaaaggg
     2821 gatacgggaa aacgtaaaac cttcgtaaac accacgctcg gtgagacgtg ggaggcgaaa
     2881 attggcgaac gtccggatgc tgaagtgatg gcagagcgga aagagcatta ttcagcgccc
     2941 gttcctgacc gtgtggctta cctgaccgcc ggtatcgact cccagctgga tcgctacgaa
     3001 atgcgcgtat ggggatgggg gccgggtgag gaaagctggc tgattgatcg gcagattatt
     3061 atgggccgcc acgacgatga acagacgctg ctgcgtgtgg atgaggccat caataaaacc
     3121 tatacccgcc ggaatggtgc agaaatgtcg atatcccgta tctgctggga tattggcggg
     3181 attgacccga ccattgtgta tgaacgctcg aaaaagcatg ggctgttccg ggtgatcccc
     3241 attaaagggg catccgtcta cggtaagccg gtggccagca tgcctcgtaa gcgaaacaaa
     3301 aacggggttt accttaccga aatcggtacg gataccgcga aagagcagat ttataaccgc
     3361 ttcacactga cgccggaagg ggatgaaccg cttcccggtg ccgttcactt cccgaataac
     3421 ccggatattt ttgatctgac cgaagcgcag cagctgactg ctgaagagca ggtcgaaaaa
     3481 tgggtggatg gcaggaaaaa aatactgtgg gacagcaaaa agcgacgcaa tgaggcgctc
     3541 gactgcttcg tttatgcgct ggcggcgctg cgcatcagta tttcccgctg gcagctggat
     3601 ctcagtgcac tgctggcgag cctgcaggaa gaggatggtg cagcaaccaa caagaaaaca
     3661 ctggcagatt acgcccgtgc cttatccgga gaggatgaat gacgcgacag gaagaacttg
     3721 ccgctgcccg tgcggcactg catgacctga tgacaggtaa acgggtggca acggtacaga
     3781 aagacggacg gcgagtggag tttacggcca cttccgtgtc tgacctgaaa aaatacattg
     3841 cggagctgga ggtgcagacc ggcatgacac agcgacgcag gggacctgca ggattttatg
     3901 tatgaaaacg cccaccattc ccacccttct gggaccggac ggcatgacat cgctgcgtga
     3961 atatgccggt tatcacggcg gtggcagcgg atttggtggg cagttgcggg cgtggaaccc
     4021 accgggtgaa agtgtggatg cagccctgct gcccaacttt acccgtggca atgcccgcgc
     4081 agacgatctg gtacgcaata acggctatgc tgccaacgcc atccagctgc atcaggatca
     4141 tatcgtcggg tcttttttcc ggctcagtca tcgcccaagc tggcgctatc tgggcatcgg
     4201 ggaggaagac gcccgtgcct tttcccgcga ggttgaagcg gcatggaaag agtttgccga
     4261 ggatgactgc tgctgcattg acgttgagcg aaaacgcacg tttaccatga tgattcggga
     4321 aggtgtggcc atgcacgcct ttaacggtga actgttcgtt caggccacct gggataccag
     4381 ttcgtcgcgg cttttccgga cacagttccg gatggtcagc ccgaagcgca tcagcaaccc
     4441 gaacaatacc ggcgacagcc ggaactgccg tgccggtgtg cagattaatg acagcggtgc
     4501 ggcgctggga tattacgtca gcgaggacgg ctatcctggc tggatgccgc agaaatggac
     4561 atggataccc cgtgagttac ccggcgggcg cgcctcgttc attcacgttt ttgaacccgt
     4621 ggaggacggg cagacccgcg gtgcaaatgt gttttacagc gtaatggagc agatgaagat
     4681 gctcgacacg ctgcagaaca cgcagctgca gagcgccatt gtgaaggcga tgtatgccgc
     4741 caccattgaa agtgagctgg atacgcagtc agcgatggat tttattctgg gcgcgaacag
     4801 taaggagcag cgggaaaggc tgacgggctg gattggtgaa attgccgcgt attacgccgc
     4861 agcaccggtc cgtctgggag gcgcaaaagt gccgcacctg atgccggggg actcactgaa
     4921 cctgcagacg gctcaggaca cggataacgg ctactccgtg tttgagcagt cactgttgcg
     4981 gtatatcgct gccgggctgg gtgtctcgta tgagcaactt tcccggaatt acgcccagat
     5041 gagctactcc acggcacggg ccagtgcgaa cgagtcgtgg gcgtacttta tggggcggcg
     5101 aaaattcgtc gcatcccgtc aggcgagcca gatgtttctg tgctggctgg aagaggccat
     5161 cgttcgccgc gtggtgacgt taccttcaaa agcgcgcttc agctttcagg aagcccgcag
     5221 tgcctggggg aactgcgact ggataggctc cggtcgtatg gccatcgatg gtctgaaaga
     5281 agttcaggaa gcggtgatgc tgatagaagc cggactgagc acctacgaga aagagtgcgc
     5341 gaaacgcggt gacgactatc aggaaatttt tgcccagcag gtccgtgaaa cgatggagcg
     5401 ccgcgcagct ggtcttaaac cgcccgcctg ggcggctgcg gcatttgaat ccgggctgcg
     5461 acaatcaaca gaggaggaga agagtgacag cagagctgcg taatctcccg catattgcca
     5521 gcatggcttt taatgagccg ctgatgcttg aacccgccta tgcgcgggtt ttcttttgtg
     5581 cgcttgcagg ccagcttggg atcagctgcc tgacggatgc agtatccggc gacagcctga
     5641 ctgccggaga ggcacccgcg gcgctggcgt tatccggtga tgatgacgga ccacgacagg
     5701 cccgcagtta tcaggtaatg aacggcatcg ccgtgctgcc ggtgtccggc acgctggtca
     5761 gccggacgcg ggcgctgcag ccgtactcgg ggatgaccgg ttacaacggc attatcgccc
     5821 gtctgcaaca ggctgccagc gacccgatgg tggacggcat tctgctcgat atggacacac
     5881 cgggcggaat ggtggcggga gcatttgact gcgctgacat catcgcccgt gtgcgtgaca
     5941 taaagccggt atgggcgctg gccaatgaca tgaactgcag cgcaggtcag ctgcttgcca
     6001 gtgccgcctc ccggcgtctg gtcacgcaga ccgcccggac aggctccatc ggcgtcatga
     6061 tggctcacag taattacggc gctgcactgg agaaacaggg cgtggaaatc acgctgattt
     6121 acagcggcag ccataaggtg gatggcaacc cctacagcca tctaccggat gatgtccggg
     6181 aaacattgca gtcccggatg gatgcaaccc gccggatgtt tgcacagaag gtgtcggcat
     6241 ataccggcct gtccgtgcag gctgtgctgg ataccgaggc tgcagtgtac agcggtcagg
     6301 aggccattga tgccggactg gctgatgaac ttgttaacag taccgatgcg atcaccgtca
     6361 tgcgtgatgc actggatgca cgtaaatccc gtctctcagg agggcgaatg accaaagaga
     6421 ctcaatcaac aactgtttca gccactgctt cgcaggctga cgttactggc gtggtgcaag
     6481 cgacggaggg cgagaacgcc agcgcggcgc agccggacgt gaacgcgcag atcaccgcag
     6541 cggttgcggc agaaaacagt cgcattatgg ggatcctcaa ctgtgaggag gctcacggac
     6601 gcgaagaaca ggcacgcgtg ctggccgaaa cccccggtat gaccgtggaa acggcccgcc
     6661 gtattctggc cgcagcacca cagagtgcac agacgcgcag tgacactgcg ctggatcgtc
     6721 tgatgcaggg ggcaccggca ccgctggctg caggtaaccc ggcatctgat gccgttaacg
     6781 atttgctgaa cacaccagtg taagggatgt ttatgacgag caaagaaacc tttacccatt
     6841 accagccgct gggcaatagt gacccggctc ataccgcaac cgcgcccggc ggattgagtg
     6901 cgaaagcgcc tgcaatgacc ccgctgatgc tggacacctc cacccgtaag ctggttgcgt
     6961 gggatggcac caccgacggt gctgccgttg gcattcttgc ggttgctgct gaccagacca
     7021 gcaccacact gacgttctac aagtccggca cgttccgtta tgaggatgtg ctctggccgg
     7081 aggctgccag cgacgagacg aaaaaacgga ccgtgtttgc cggaacggca atcagcatcg
     7141 tttaacttta cccttcatca ctaaaggccg cctgtgcggc tttttttacg ggattttttt
     7201 atgtcgatgt acacaaccgc ccagctgctg gcggcaaatg agcagaaatt taagtttgat
     7261 ccgctgtttc tgcgtctctt tttccgtgag agctatccct tcactacgga gaaagtctat
     7321 ctctcacaaa ttccgggact ggtaaacatg gcgctgtacg tttcgccgat tgtttccggt
     7381 gaggttatcc gttcccgtgg cggctccacc tctgaattta cgccgggata tgtcaaaccc
     7441 aagcatgagg tgaatccgca gatgaccctg cgtcgcctgc cggatgaaga tccgcagaat
     7501 ctggcggacc cggcttaccg ccgccgtcgc atcatcatgc agaacatgcg tgacgaagag
     7561 ctggccattg ctcaggtcga agagatgcag gcagtttctg ccgtgctcaa gggcaaatac
     7621 accatgaccg gtgaagcctt cgatccggtt gaggtggata tgggccgcag tgcggcgaac
     7681 aacatcacac agtccggcgt cacggagtgg agcaagcgtg acaagtccac gtatgacccg
     7741 accgacgata tcgaagccta cgcgctgaac gccagcggtg tggtgaatat catcgtgttt
     7801 gacccgaaag gctgggcgct gttccgttcc ttcaaagccg tcaaggagaa gctggatacc
     7861 cgtcgcggct ctcattccga gctggagaca gcggtaaaag acctgggcaa agcggtgtcc
     7921 tataagggaa tgtatggcga tgtggccatc gtcgtgtatt ccggacagta cgtggaaaac
     7981 ggcgtcaaaa agaacttcct gccggacaac acgatggtgc tggggaacac tcaggctcgc
     8041 ggtctgcgca cctatggctg cattcaggat gcggacgcac agcgcgaagg cattaacgcc
     8101 tctgcccgtt acccgaaaaa ctgggtgacc accggcgatc cggcgcgtga gttcaccatg
     8161 attcagtcag caccgctgat gctgctggct gatcctgatg agttcgtgtc cgttcaactg
     8221 gcgtaatcat ggcccttcgg ggccattttc tctctgtgga ggagttcatg acgaaagatg
     8281 aactgattgc ccgtcttcag gagctgggtg agcaattgaa ccgcgatgtc agtttgacgg
     8341 gaacgaaaga agaactggcg cttcgtgtgg cagagctgga agaagagctt gatgacacgg
     8401 atgaaactgc cggtcaggac acccctctca gccgggaaaa tgtgctgacc gggcatgaaa
     8461 atgaggtggt atcagcgcag ccggataccg tgattcagga tacggctgaa ctggtcacgg
     8521 tcgtggcact ggtgacgctg catactgatg cacttcacgc cacgcgggat aaacctgtgg
     8581 catttgtgct gccgggaacg gcgtttcgtg tctctgccgg tgtggcagcc gaaatgacag
     8641 agcgcggcct ggccagaatg caataacggg aggcgctgtg gctgatttcg ataacctgtt
     8701 cgatgctgcc attgcccgcg ccgatgaaac gatacgcggg tacatgggaa cgtcagccac
     8761 catgacatcc ggtgagcagt caggtgcggt gatacgtggt gtttttgatg accctgaaaa
     8821 tatcagctat gccggacagg gcgtgcgcgt tgaaggctcc agcccgtccc tgtttgtccg
     8881 gactgatgag gtgcggcagc tgcggcgtgg agacacgctg accatcggtg aggaaaattt
     8941 ctgggtagat cgggtttcgc cggatgatgg cggaagttgt catctctggc ttggacgggg
     9001 cgtaccgcct gccgttaacc gtcgccgctg aaagggggat gtatggccat aaaaggtctt
     9061 gagcaggccg ttgaaaacct cagccgtatc agcaaaacgg cggtgcccgg tgccgccgca
     9121 atggccatta accgcgttgc ttcatccgcg atatcgcagt ctgcgtcaca ggttgcccgt
     9181 gagacaaagg tacgccggaa actggtaaag gaaagggcca ggctgaaaag ggccacggtc
     9241 aaaaatccgc aggccagaat caaagttaac cggggggatt tgcccgtaat caagctgggt
     9301 aatgcgcggg ttgtcctgtc ccgccgcagg cgtcataaaa aggggcagcg ttcatccctg
     9361 aaaggtggcg gcagcgtgct tgtggtgggt aaccgtcgta ttcccggcgc gtttattcag
     9421 caactgaaaa acggccggtg gcatgttatg cagcgtgtgg ccgggaaaaa ccgttacccc
     9481 attgatgtgg tgaaaatccc gatggcggtg ccgctgacca cggcgtttaa acagaatatt
     9541 gaacggatac ggcgtgaacg tcttccgaaa gagctgggct atgcgctgca gcatcaactg
     9601 agaatggtaa taaagcgatg aaacatactg aactccgtgc agccgtactg gatgcactgg
     9661 agaagcatga caccggggcg acgctttttg atggtcgccc cgctgttttt gatgaggaag
     9721 attttccggc aattgccgtt tatctcaccg gcgctgaata cacgggcgaa gagctggaca
     9781 gcgatacctg gcaggcggag ctgcatattg aagttttcct gcctgctcag gtgccggatt
     9841 cagagctgga ttcgtggatg gagtcccgga tttatccggt gatgagcgat atcccggcac
     9901 tgtcagattt gatcaccagt atggtggcca gcggctatga ctaccggcgc gacgatgatg
     9961 cgggcctgtg gagttcagcc gatctgactt atgtcattat ctatgaaatg tgaggacgct
    10021 atgcctgtac caaatcctac aatgccggtg aaaggtgccg ggaccaccct gtgggtttat
    10081 aaggggagcg gtgaccctta tgcgaacccg ctttcagacg ttgactggtc gcgtctggca
    10141 aaagttaaag acctgacgcc cggcgaactg accgctgagt cctatgacga cagctatctc
    10201 gatgatgaag atgcagactg gactgcgacc gggcaggggc agaaatctgc cggagatacc
    10261 agcttcacgc tggcgtggat gcccggagag caggggcagc aggcgctgct ggcgtggttt
    10321 aatgaaggtg atacccgtgc ctataaaatc cgcttcccga acggcacggt cgatgtgttc
    10381 cgtggctggg tcagcagtat cggtaaggcg gtgacggcga aggaggtgat tacccgcacg
    10441 gtgaaggtca ccaatgtggg acgtccgtcg atggcagaag atcgcagcac ggtgacggcg
    10501 acgaccggca tgacggtgac acccgccagt gcttccgtag tgaaagggca gagcaccacg
    10561 ctgaccgtgg cattccagcc ggatggcgca accgacaaga gcttccgtgc ggtgtctgcg
    10621 gataaaacaa aagccaccgt gtcggtcagt ggtatgacca tcaccgtgaa aggtgttgct
    10681 gcaggcaagg tcaacattcc ggtcgtatct ggtaatggtg agtttgctgc ggttgcagaa
    10741 atcaacgtca ccgccagtta atccggagag tcagcgatgt tcctgaaaac cgaatcattt
    10801 gaacataacg gtgtgaccgt cacgctttct gaactgtcag ccctgcagcg tattgagcat
    10861 ctcgacctga tgaaacggca ggcagaacag gcggagtcag acagcaaccg gaagtttact
    10921 gtggaagacg ccatcagaac cggcgctttt gtggttgcga tgtccctgtg gcataaccat
    10981 ccgaagaaga cgcagatgcc gtccatgaat gaagccgtta aacagattga gcaggaagtg
    11041 cttaccacct ggcccacaga ggcaatttct catgctgaaa acgtggtgta ccggctgtcc
    11101 ggtatgtatg agtttgtggt gaatgatgct cctgaacagg cagaggacgc cgggcctgca
    11161 gagcctgttt ctgcgggaaa gtgttcgacg gtgagctgag ttttgccctg aaactggcgc
    11221 gtgagatggg gcgacccgac tggcgcgcca tgcttgccgg gatgtcatcc acggagtatg
    11281 ccgactggca ccgcttttac agtacccatt attttcatga tgttctgctg gatatgcact
    11341 tttccgggct gacgtacacc gtgctcagcc tgtttttcag cgatccggag atgcatccgc
    11401 tggatttcag tctgctgaac cggcgcgagg ctgacgaaga gcctgaagat gatgtgctga
    11461 tgcagaaagc ggcagggctt gccggaggtg tccgctttgg cccggacggg aatgaagtta
    11521 tccccgcttc cccggatgtg gcggacatga cggaggatga cgtaatgctg atgacagtat
    11581 cagaagggat cgcaggagga gtccggtatg gctgaaccgg taggcgatct ggtcgttgat
    11641 ttgagtctgg atgcggccag atttgacgag cagatggcca gagtcaggcg tcatttttcc
    11701 ggtacggaaa gtgatgcgaa aaaaacagcg gcagtcgttg aacagtcgct gagccgacag
    11761 gcgctggctg cacagaaagc ggggatttcc gtcgggcagt ataaagccgc catgcgtatg
    11821 ctgcctgcac agttcaccga cgtggccacg cagcttgcag gggggcagaa tccctggctg
    11881 atcctgctgc aacagggtgg tcaggttaag gactccttcg gcgggatgat ccccatgttc
    11941 agggggcttg ccggcgcgat caccctgccg atggtggggg ccacctcgct ggcggtggcg
    12001 accggtgcgc tggcgtatgc ctggtatcag ggcaactcaa ccctgtccga tttcaacaaa
    12061 acgctggtcc tttccggtaa tcaggcggga ctgacggcag atcgtatgct ggtcctgtcc
    12121 agagccgggc aggcggcagg gctgacgttt aaccagacca gcgagtcact cagcgcactg
    12181 gttaaggcgg gggtaagcgg tgaggctcag attgcgtcca tcagccagag tgtggcgcgt
    12241 ttctcctctg catccggcgt ggaggtggac aaggtcgctg aagccttcgg gaagctgacc
    12301 acagacccga cgtcggggct gacggcgatg gcacgccagt tccataacgt gacggcggag
    12361 cagattgcgt atgttgctca gttgcagcgt tccggcgatg aagccggggc attgcaggcg
    12421 gcgaacgagg ccgcaacgaa agggtttgat gaccagaccc gccgcctgaa agagaacatg
    12481 ggcacgctgg agacctgggc agacaggact gcacgtgcat tcaaatccat gtgggatgcg
    12541 gtgctggata ttggtcgtcc tgataccgcg caggagatgc tgattaaggc agaggctgcg
    12601 tttaagaaag cagacgacat ctggaatctg cgcaaggatg attatttcgt taacgatgaa
    12661 gcacgggcgc gttactggga tgatcgtgaa aaaaaacgcc tggagcggga tgcagcccaa
    12721 aagagggtag atcaacagcg tcaacaggac aaaaatgcgc agcagcagag cgataccgaa
    12781 gcgtcacggc tgaaatatac cgaagaggcg cagaaggctt acgaacggct gcagacgccg
    12841 ctggagaaat ataccgcccg tcaggaagaa ctgaacaagg cactgaaaga cggaaaaatc
    12901 ctgcaggcag attacaacac gctgatggcg gcggcgaaaa aggattatga agcgacgctg
    12961 aaaaagccga aacagtccgg cgtgaaggtg tatgcgggcg atcgtcagga agacagtgct
    13021 catgcggccc tgctgacgct tcaggcagaa ctccggacgc tggagaagca tgccggagcg
    13081 aatgagaaaa tcagccagca gcgccgggat ttgtggaagg cagaaagtca gttcgcggta
    13141 ctggaggagg cggcacaacg tcgccagctg tccgcacagg agaaatccct gctggcgcat
    13201 aaagacgaga cgctggagta caaacgccag ctggctgcac ttggtgacaa ggttacgtat
    13261 caggagcacc tgaatgcgct ggcgcagcag gcggataaat tcgcacagca gcaacgggca
    13321 aaacgggcag ccattgatgc gaaaaaccgg gggctgactg accggcaggc agcgcgggaa
    13381 gccacggaac agcgcctgaa ggaacagtat ggcgataatc ctctggcgct gaataacgtc
    13441 atgtcagagc agaaaaagac ctgggcggct gaagaccagc ttcgcgggag ctggatggca
    13501 ggcctgaagt ccggctggag tgagtgggaa gagagcgcca cggacagtat gtcacaggtt
    13561 aaaagtgcag ccacgcagac ctttgatggt attgcacaga atatggcggc gatgctgacc
    13621 ggcagtgaac agaactggcg cagcttcacc cgctccgtgc tgtccatgat gacagaaatt
    13681 ctgcttaagc aggcaatggt ggggattgtc gggagtatcg gcagcgccat tggcggggct
    13741 gttggtggcg gcgcatccgc gtcaggtggt acagccattc aggccgctgc ggcgaaattc
    13801 cattttgcaa ccgggggatt tacgggaacc ggcggcaaat atgagccagc ggggattgtt
    13861 caccgtggtg aatttgtctt cacgaaggag gcaaccagcc ggattggcgt ggggaatctc
    13921 taccggctga tgcgcggcta tgcggaaggt ggttatgtgg gcggtgccgg aagtccggcg
    13981 cagatgcggc gggctgaagg cattaatttt aatcagaaca atcacgtggt gattcagaac
    14041 gacggtacga atggtctgcc aggtccacag atgatgaagg ccgtgtatga catggcccgc
    14101 aagggtgccc gtgatgaaat tcagacacag atgcgtgatg gtggcctgtt ctccggaggt
    14161 ggacgatgaa aaccttccgc tggaaagtga aacccggtat ggatgtggct tcggcccctt
    14221 ctgtaagaaa ggtgcgcttt ggtgatggct attcccagcg agcgcctgcc gggctgaatg
    14281 ccaacctgaa aacgtacagc gtgacgcttt ctgtcccccg tgaggaggcc atggtactgg
    14341 agtcgtttct ggaagagcac gggggctgga aagcctttct gtggacgccg ccttatgagt
    14401 ggcggcagat aaaggtgacc tgcgcaaaat ggtcgtcgcg ggtcagtatg ctgcgtgttg
    14461 agttcagcgc agagtttgaa caggtggtga actgatgcag gatatccggc aggaaacact
    14521 gaatgaatgc acccgtgcgg agcagtcggc cagcgtggtg ctctgggaaa tcgatctgac
    14581 agaggtcggt ggagaacgtt attttttctg taatgagcag aacgaaaaag gtgagccggt
    14641 cacctggcag gggcgacagt atcagccgta tcccattcag gggagtggtt ttgaactgaa
    14701 tggcaaaggc accagtacgc gccccacgct gatggtttct aacctgtacg gtatggtcac
    14761 cgggatggcg gaagatctac agagtctggt cggcggaacg gtggtccggc gtaaggttta
    14821 cgcccgtttt ctggatgcgg tgaacttcgt caacggaaac agtgacgccg atccggagca
    14881 ggaggtgatc agccgctggc gcattgagca gtgcagcgaa ctgagcgcgg tgagtgcctc
    14941 ttttgtactg tccacgccga cggaaacaga tggcgctgtt tttccgggac gtatcatgct
    15001 ggccaacacc tgcacctgga cctatcgcgg cgatgagtgc ggttatcacg gtccggcggt
    15061 cgcggatgaa tatgaccagc caacgtccga catcacgaag gataaatgca gcaaatgcct
    15121 gagtggctgt aagtttcgca ataacgtcgg caactttggc ggcttccttt ccattaacaa
    15181 actttcgcag taaatcccat gacagagaca gaatcagcga ttctggcgca cgcccggcga
    15241 tgtgctccag cggagtcgtg cggcttcgtg gtaagaacgc cggaggggga aagatatttc
    15301 ccctgcgtga atatctccgg tgagccggag gcgtatttcc gtatgtcgcc ggaagactgg
    15361 ctgcaggcag agatgcaggg tgagattgtg gcactggtcc acagccaccc cggtggtctg
    15421 ccctggctga gtgaggccga ccggcggctg caggtgcaga gtgatttgcc gtggtggctg
    15481 gtctgccggg gggcgattca caagttccgc tgtgtgccac atcttaccgg gcggcgcttt
    15541 gagcacgggg tgacggactg ttacacgctg ttccgggatg cttaccatct ggcggggatt
    15601 gagatgccgg attttcatcg tgaggatgac tggtggcgtc acggtcagaa tctctatctg
    15661 gataatctgg aggccacagg gctgtatcag gtgccgttgt cagcggcgca gccgggcgat
    15721 gtgctgctgt gctgttttgg ttcatcggtg ccgaatcatg ccgccattta ctgtggtgac
    15781 ggcgagctgc tgcaccatat tcctgaacaa ctgagcaaac gagagaggta taccgacaaa
    15841 tggcagcgac gcacacactc cctctggcgt caccgggcat ggcacgcatc tgcctttacg
    15901 gggatttgca acgatttggc cgccgcatcg accttcgtgt gaaaacgggt gccgaagcca
    15961 tccgggcgat ggccacacag ctccctgcgt ttcgtcagaa gctgagcgac ggctggtatc
    16021 aggtgcgtat tgccgggcag gatgttagca cgtccggatt aacggcgcag ttacatgagg
    16081 ctctgcctga cggcgctgtg attcatattg ttcccagagt cgccggggcc aagtcaggtg
    16141 gtgtattcca gattgtcctg ggagcagccg ccattgccgg atcattcttt accgccggag
    16201 ccacccttgc agcatggggg gctgccattg gtgccggtgg tatgaccggc attctgtttt
    16261 ctctcggtgc cagtatggtg ctcggcggtg tggctcagat gctggcaccg aaagccagaa
    16321 ctccccgcac acagacaacg gataacggta agcagaacat ctatttctcc tcactggata
    16381 acatggttgc ccagggcaat gttatgcctg ttctgtatgg tgaaatgcgc gtggggtcac
    16441 gtgtggtatc tcaggagatc agcacggcag acgaagggga cggtggtcag gttgtggtga
    16501 ttggtcgctg atgcaaaatg ttttatgtga aatcgcctcc gggcggtttt atcgtttatg
    16561 gagcatgacg aatgggtaaa ggcagcagta aggggcatac cccgcgcgaa gcgaaggaca
    16621 acctgaagtc cacgcagctg ctgagtgtga tcgatgccat cagcgaaggg ccggttgaag
    16681 gtccggtgga tggattaaaa agcgtgctgc tgaacagtac gccggtgctg gacagtgagg
    16741 ggaataccaa catctccggc gtcacggtgg tgttccgggc aggtgagcag gagcagtcac
    16801 cgccggaggg atttgaatcc tccggctccg agacggtgct gggtacggaa gtgaaatatg
    16861 acacgccgat cacccggacc atcacgtcgg caaacattga ccgactgcgt tttaccttcg
    16921 gtgtacaggc actggtggaa accacctcaa agggagacag gaatccgtct gaagttcgcc
    16981 tgctggttca gatacagcgt aacggtggct gggtgacgga aaaagacatc accattaagg
    17041 gcaaaaccac ctcgcagtat ctggcctcgg tggtggtgga taacctgccg ccgcgcccgt
    17101 ttaatatccg gatgcgcaga atgacgccgg acagcaccac agaccagctg cagaacaaaa
    17161 cgctctggtc gtcatacacc gaaatcatcg atgtgaaaca gtgctacccg aacacggcac
    17221 tggtcggcgt acaggtggat tcggagcagt tcggcagcca gcaggtgagc cgtaattatc
    17281 atcttcgcgg gcgcattctg caggtgccgt cgaactataa tccgcagacg cggcaataca
    17341 gcggtatctg ggacggaacg ttaaaaccgg catacagcaa caacatggcc tggtgtctgt
    17401 gggatatgct gacccatccg cgctatggca tggggaaacg tcttggtgcg gcagatgtgg
    17461 ataaatgggc gctgtatgtc atcggccagt actgtgacca gtcggtgccg gacggctttg
    17521 gcagcacgga gccgcgcatc acctgtaatg cctggctgac cacacagcgc aaggcgtggg
    17581 atgtgctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac gggcagacgc
    17641 tgacgttcgt gcaggaccga cagtcggata aggtgtgggc ctataaccgc agtaatgtgg
    17701 tgatgccgga tgatggcgcg ccgttccgct acagtttcag cgccctgaaa gaccgccata
    17761 atgccgttga ggtgaactgg attgacccgg ataacggctg ggagacggcg acagagcttg
    17821 tggaggatac gcaggccatt gtccgttacg gtcgtaacgt cacgaagatg gatgcctttg
    17881 gctgtaccag ccgggggcag gcacaccgcg ccgggctgtg gctgattaaa acggaactgc
    17941 tggagacgca gaccgtggat ttcagcgtgg gtgctgaagg gcttcgtcat gtaccgggcg
    18001 atgtcattga aatctgcgat gatgactatg ccggtatccg caccggcggg cgcgtgctgg
    18061 cggtgaacag ccagacccgg acgctgacgc tcgaccgtga aatcacgctg ccatcctccg
    18121 gcaccacgct gataagcctg gttgacgggc aggggaatcc ggtcagcgtg gaggtccagt
    18181 ccgtcaccga cggcgtgaag gtaaaagtga gccgtgttcc tgacggtgtt gctgaataca
    18241 gcgtgtgggg gctgaagctg ccgacgctgc accagcgatt gttccgctgc gtgagtatcc
    18301 gtgagaacga cgacggcacg tatgccatca ccgccgtgca gcatgtgccg gaaaaagaag
    18361 ccatcgtgga taacggggcg cactttgacg gcgaccagag cggcacggtg aatggtgtca
    18421 cgccgccagc ggtgcagcac ctgactgccg aagtcaccgc agacagcggg gaatatcagg
    18481 tgctggcgcg ctgggacacg ccgaaggtgg tgaagggcgt gagcttcctg ctccgtctga
    18541 ccgtaacagc ggatgacggc agtgagcggc tggtcagcac ggcccggacg acggaaacca
    18601 cttaccgctt cacacaactg gcgctgggga actacaggct gacagtccgg gcagtaaatg
    18661 cgtgggggca gcagggcgat ccggcgtcgg tatcgttccg gattgccgca ccggcagcgc
    18721 tgtcgcggat tgagctgacg ccgggctatt ttcagataac cgccacgccg catcttgcgg
    18781 tttatgatcc gacggtacag tttgagttct ggttctcgga aaagcggatt gcggatatca
    18841 ggcaggttga aaccacagca cgctatcttg gcacggcgct gtactggata gccgccagta
    18901 tcaatatcaa accgggccat gattattact tttatatccg cagtgtgaac accgttggca
    18961 aatcggcatt cgtggaggct gttggtcagc cgagtgatga tgcatccggt tatctggatt
    19021 ttttcaaagg cgagataggg aaaacccatc tggctcagga gctgtggacg cagattgata
    19081 acggtcagct tgcgcctgac ctggctgaaa tcaggacgtc cattacggat gtcagcaatg
    19141 aaatcacgca gaccgtcaat aagaaactgg aagaccagag tgcggcaatt cagcagatac
    19201 agaaggttca ggttgataca aataataacc tgaacagcat gtgggctgtg aagctgcagc
    19261 agatgcagga cggacgcctt tatatcgcgg gtattggtgc cggtattgag aacacccctg
    19321 acggcatgca gagtcaggtg ctgctggcgg cggacaggat tgcgatggtt aatcctgcga
    19381 atggcaacac aaaaccgatg tttgttggtc agggcgatca gatattcatg aacgacgtgt
    19441 tcctgaaacg cctgacggcc cccaccatta ccagcggtgg aaatccatcg gcattttccc
    19501 tgactccgga cggaaagctg accgctaaaa atgcggatat cagtggcagt gtgaatgcga
    19561 acgccgggac gctcaacaat gtcacggtaa atgaaaactg tacgattaag ggcatgctgg
    19621 aggcgactca ggtcagaggt gacttcgtta aagctgtatc caaatcattt ccgaaacagg
    19681 ctggtacgtg gggtaacacg gaaacaccaa acgggacggt tacagtcacc ataagcgatg
    19741 atcataactt tgaccgccag attattattc cgcccattat ttttaacggt atagcgtatg
    19801 acgatccggg gagcggaaat aacccaggag gcacgcgata cacgggttat ggttttgaag
    19861 ttcgcaaaaa cggcgtatta atcgcatcca gagaaactaa aggtgccatt cccggtagtt
    19921 acagtgcagt tattgatatg cctagtggtg gtggtagcgt cactctggag tttaagattt
    19981 tccagaaagg caatcagggg gcaggcaata tcaccgactg tacggtgatt gtgaccaaaa
    20041 aagcggcttc cggcatcagt attcgttgaa atatttataa ccccaataaa tggcgtcagg
    20101 aatgacgcct tttttattgc agaaaagcga gaggtaatta tgcgtaaagt ttgtgcagcc
    20161 attttgtccg cagccatttg cctggccgta tccggtgcgc ctgcatgggc gtctgaacat
    20221 cagtccacgc tgagcgcggg gtatcttcat gcccggacca acgttcccgg cagtgatgat
    20281 ctgaacggga ttaacgtgaa ataccgttat gagtttacgg atacgctggg gctggtgacg
    20341 tcattcagct atgcaggaga caagaatcgc cagctgaccc gttacagcga tacccgctgg
    20401 catgaagatt ccgtgcgtaa ccgctggttc agcgtgatgg cggggccgtc tgtgcgcgtg
    20461 aatgaatggt tcagcgcgta tgcgatggcg ggtgtggctt acagccgtgt gtcgactttt
    20521 tccggggatt atatccgcgt aactgacaat aaggggaaaa cgcacgatgt gctgaccgga
    20581 agtgatgacg gtcgccacag caacacgtct ctggcgcact gacggtttca tcgtgggtgt
    20641 cggttataaa ttctgattag ccaggtaaca cagtgttatg acagcccgcc ggttcaggcg
    20701 ggcttttttg tggggtgaat atggcagtaa agatttcagg tgtactgaaa gacggcacag
    20761 gaaaaccggt acagaactgc acaatccagc tgaaagcaaa acgtaacagc accacggtgg
    20821 tggtgaacac gctggcctca gaaaatccgg atgaagccgg gcgttacagc atggacgttg
    20881 agtacggtca gtacagcgtt attctgttgg tggaaggatt cccgccgtca catgccggga
    20941 ccatcaccgt gtatgaagat tcccgacccg gtacgctgaa tgattttctc ggtgccatga
    21001 cggaggatga tgcccgtccg gaggcactgc gccgttttga gctgatggtg gaagaggtgg
    21061 cgcgtaacgc gtccgcggtg gcacagaaca cggcagccgc gaagaaatca gccagcgatg
    21121 ccagcacatc agcccgtgag gcggcaaccc atgcgactga tgctgcagat tcagcacgtg
    21181 cagccagcac gtcagccgga caggccgctt cgtcggctca gtcagcgtct tccagcgcag
    21241 gaacggcatc aacaaaggcc actgaagcct caaaaagtgc tgccgctgca gagtcctcaa
    21301 aaagcgcggc ggctaccagt gccggtgcgg cgaaaacgtt agaaacgaat gcggcagcgt
    21361 cacaacaatc agcagccact tctgcatcca ccgcgaccac gaaagcgtca gaagctgcca
    21421 cctcagcccg ggatgcgtcg gcttcaaaag aggcggcaaa atcatcagaa acgaacgcat
    21481 catcaagcgc cagtagcgca gcttcctcgg cgacggcggc tgcaaattct gcgaaggcgg
    21541 ccaaaacgtc cgagacgaac gccaggtctt ctgaaacggc agcgggacag agtgcctcag
    21601 ctgcggcagg ctcaaaaaca gcggctgcgt cgtctgccag cgccgcgtca acaagtgccg
    21661 ggcaggcctc agccagtgcc accgccgccg gaaaatcggc agaaagcgcc gcatcatccg
    21721 cttcaacagc cacaacgaag gctggcgaag ccactgagca ggccacggca gcagcgaggt
    21781 cagcttccgc agcgaagaca tccgaaacga acgcgaaagc gtcggaaacc cgtgcagaat
    21841 cctcaaaaac ggctgccgca tcgtccgcca gttcggcggc gtcatcggca tcatcggcgt
    21901 ctgcttcaaa agatgaggcg accagacagg cgtcagcagc gaagggcagc gccacgacgg
    21961 catccacgaa ggcgacagag gctgctggta gtgcgacggc agcagctcag agcaaaagta
    22021 cggcggaatc tgcagcaacg cgcgctgaga cagcggcaaa acgggcagag gatattgcat
    22081 ccgccgtggc gcttgaggat gcgagcacga cgaaaaaggg gatagtacag ctcagtagtg
    22141 cgactaacag cacttccgag tcactggcgg caacaccaaa agccgttaag gccgcgtatg
    22201 acctggctaa cgggaaatac accgcacagg atgcaacgac agcacagaaa gggataatcc
    22261 agctaagcag cgcgaccaac agcacgtctg aaacgctggc ggcaacgcca aaggcagtaa
    22321 aagcagccaa tgacaatgct gagaaacgtc tgcagaaaga tcagaacggt gcggatatcc
    22381 ctggcaaaga cacctttacg aaaaatattg gtgcctgccg tgccttcggt gggtcagtaa
    22441 gcacaacaac aggaaactgg acgactgcac agtttatcga gtggctggat tctcagggag
    22501 catttaacca tccatactgg atgtgcaagg gttcctggtc ttatggcaat aataaaatca
    22561 ttactgatac tggctgcggt aatattcatc tcgccggagc tgtcattgaa gtaatgggga
    22621 taaagtcagc gatgacgatc cgcattacca ctccgaccac atccagcggt ggcggaacaa
    22681 ccaacgccca gtttacctat attaatcacg gaacagatta ttcacctggc tggcgaaggg
    22741 actataactc cagaaataag ccaacggcat cagagatcgg ggcgctaccg tcaggtggaa
    22801 cagcagtatc atcagttaat ctggcttcaa aaggtcgggt agccgcgctg acagacaata
    22861 cgcagggggc agcaggtctt gagttatacg aggtgtataa caacggatat ccaacagcgt
    22921 atggaaatat cattcacctg aaagggatga cagccgttgg cgaaggtgag ttactcatcg
    22981 gctggagtgg tacaagcggt gctcatgctc cggcatttat tcgttcacga cgggatacga
    23041 ccgacgcaaa ctggtcgccg tgggcgcagc tttacacctc ggctcatcct cctgcagagt
    23101 tttatccagt cggtgcacca atcccgtggc catcagatac cgttccgtct ggttatgccc
    23161 tgatgcaggg gcagactttt gacaaatctg cttacccgaa acttgcagcc gcttatccgt
    23221 caggcgtgat ccctgatatg cgtggctgga cgattaaggg caagcccgcc agtggtcgtg
    23281 ccgtattgtc tcaggaacag gatggcatta aatcgcacac ccacagcgcc agcgcatcca
    23341 gtacggattt ggggacgaaa accacatcgt cgtttgatta cggcactaaa tccacgaata
    23401 acaccggggc gcatacgcac agtctgagcg gctctacaag ttcagctggg gcccaccaac
    23461 atagtcagac tgggcccaga acgaatagcg ggagtcaacc tactggaatg tttccggcag
    23521 ggagtactca agttagcggg actaatcagg ttggtatcag tggctcatta accagtggca
    23581 cctcccaatg ggtaggtaaa tcatcttcag aaggaaacca tacacactca ctgtccggca
    23641 cagcagcatc tgcgggtgca cacgcgcata ctgtcggtat tggtgctcat acgcactctg
    23701 ttgcgattgg ttcacatgga cacaccatca ccgttaacgc tgctggtaac gcggaaaaca
    23761 ccgtcaaaaa catcgcattt aactatattg tgaggctcgc ataatggcat tcagaatgag
    23821 tgaacaagca cggaccataa aaatttataa cctgctggcc ggaactaatg aatttattgg
    23881 tgaaggtgac gcatatattc cgcctcatac aggtctgcct gcaaacatcg tggcatctcg
    23941 ttgaagatca tcggggtaaa acggtttatg acgtggcttc cggcgacgcg ttatttattt
    24001 ctgaactcgg ctcattaccg gaaaatgtca cctggttatc cccggaaggg gagtttcaga
    24061 agtggaacgg cacagcctgg gtgaaggata cggaagcaga aaaactgttc cggatccggg
    24121 aggcggaaga aacaaaaaac agcctgatgc aggtagccag tgagcatata gcgccgcttc
    24181 aggatgctgt ggatctggag atcgcaacgg agaaagaaac cttgttgctg gaagcctgga
    24241 aaaagtatcg ggtgttgctg aaccgtgttg atacatcaac tgcaccggat attgaatggc
    24301 cagtagcacc tatagggtaa attcgtaatg attacctaaa tacgttattc ttttgttaaa
    24361 aaagtgattt tgctataggt agggagtatg ttcttggatt aagaataaaa aatcgccatg
    24421 aaagagcttc atggcgagta cataaacgta caaaatgtga tgaaagtaga gtggcgtaag
    24481 ccactatatt aagatatgac aatagcgaag aaaagtaaat aacattttat ggacgtttaa
    24541 ttgttaatta taaaaactag agtgcccgat attgtttatc ttgctttaaa ata
//
LOCUS       DAZPLI010000051        24420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZPLI010000051 DAZPLI010000000
VERSION     DAZPLI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.051"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            38..754
                     /locus_tag="ACHUU3_001967"
     CDS             38..754
                     /locus_tag="ACHUU3_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR2082042.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFLGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            784..1284
                     /locus_tag="ACHUU3_001968"
     CDS             784..1284
                     /locus_tag="ACHUU3_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFR2082043.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1376..4060
                     /locus_tag="ACHUU3_001969"
     CDS             1376..4060
                     /locus_tag="ACHUU3_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR2082044.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4057..5145
                     /locus_tag="ACHUU3_001970"
     CDS             4057..5145
                     /locus_tag="ACHUU3_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR2082045.1"
                     /translation="MRNRLIAAILGLFGTLIGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5228..6525)
                     /gene="exuT"
                     /locus_tag="ACHUU3_001971"
                     /pseudo
     CDS             complement(5228..6525)
                     /gene="exuT"
                     /locus_tag="ACHUU3_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            7008..8420
                     /gene="uxaC"
                     /locus_tag="ACHUU3_001972"
     CDS             7008..8420
                     /gene="uxaC"
                     /locus_tag="ACHUU3_001972"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFR2082046.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8435..9922
                     /gene="uxaA"
                     /locus_tag="ACHUU3_001973"
     CDS             8435..9922
                     /gene="uxaA"
                     /locus_tag="ACHUU3_001973"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR2082047.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10005..10556
                     /locus_tag="ACHUU3_001974"
     CDS             10005..10556
                     /locus_tag="ACHUU3_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR2082048.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10561..11805)
                     /gene="sstT"
                     /locus_tag="ACHUU3_001975"
     CDS             complement(10561..11805)
                     /gene="sstT"
                     /locus_tag="ACHUU3_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR2082049.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12421..13386)
                     /gene="alx"
                     /locus_tag="ACHUU3_001976"
     CDS             complement(12421..13386)
                     /gene="alx"
                     /locus_tag="ACHUU3_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR2082050.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(13669..14655)
                     /locus_tag="ACHUU3_001977"
     CDS             complement(13669..14655)
                     /locus_tag="ACHUU3_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR2082051.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14734..15417)
                     /locus_tag="ACHUU3_001978"
     CDS             complement(14734..15417)
                     /locus_tag="ACHUU3_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR2082052.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(15494..15997)
                     /locus_tag="ACHUU3_001979"
     CDS             complement(15494..15997)
                     /locus_tag="ACHUU3_001979"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR2082053.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            16082..17218
                     /gene="rlmG"
                     /locus_tag="ACHUU3_001980"
     CDS             16082..17218
                     /gene="rlmG"
                     /locus_tag="ACHUU3_001980"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR2082054.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            17503..17817
                     /gene="higB"
                     /locus_tag="ACHUU3_001981"
     CDS             17503..17817
                     /gene="higB"
                     /locus_tag="ACHUU3_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR2082055.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            17814..18230
                     /gene="higA"
                     /locus_tag="ACHUU3_001982"
     CDS             17814..18230
                     /gene="higA"
                     /locus_tag="ACHUU3_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR2082056.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(18275..20293)
                     /gene="fadH"
                     /locus_tag="ACHUU3_001983"
     CDS             complement(18275..20293)
                     /gene="fadH"
                     /locus_tag="ACHUU3_001983"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR2082057.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLNVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(20519..22870)
                     /gene="ygjK"
                     /locus_tag="ACHUU3_001984"
     CDS             complement(20519..22870)
                     /gene="ygjK"
                     /locus_tag="ACHUU3_001984"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR2082058.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22887..23964)
                     /gene="ygjJ"
                     /locus_tag="ACHUU3_001985"
                     /pseudo
     CDS             complement(22887..23964)
                     /gene="ygjJ"
                     /locus_tag="ACHUU3_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(24098..>24420)
                     /locus_tag="ACHUU3_001986"
     CDS             complement(24098..>24420)
                     /locus_tag="ACHUU3_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2082059.1"
                     /translation="ASMLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIA
                     IFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPA
                     SNVD"
BASE COUNT         6170 a         6199 c         6256 g         5795 t
ORIGIN      
        1 caactttttg gaaacaatat tgtaagatca aatttatatg gtggcgaaaa ttaatctctt
       61 atcacggctt acgccgcttt tattcgtgtt cgctcctttt ttggcacagt caaatatgac
      121 tgtttatcct atggctgttt ccattaatag tcagggtgaa ggcaacgttc gcgtaatatc
      181 taaaagtaat gaagttcagt acatcaaggc gacagtattc cgtatcgata atccatcgac
      241 gcctcaggaa aatgaagttg agattaaatc cggtgatgca aatcatctgg ttgttatgcc
      301 acctaaattc gctttaccag ccggtagtag caaaactgtg cgctttgttg caatggaacc
      361 agagcaaaaa gaaaaaaatt atcgcgttaa atttgaagcg gttcccagta ttgatgacgt
      421 tgccacagat aaaaaagatc tctctatgca gttaacagtt aacttaattt gggggattgt
      481 tgttagtgtt ccacctcagc aacctattgc taagttagaa gtaaatgctg cccaaaaatt
      541 agttaatgca gggaatcaac gcttaaagat tttaacgatc gcttattgta aaaataatag
      601 caaagaaaat tgtaagatac agaccgtaaa taaaaacatc ttccttggtc aggaaagaaa
      661 tcttgaaagc atatctggct acgacaaaat cgttgtcaaa tataataact ggatcaccaa
      721 agataatggc gagtttgaac tggcggtcca ttaattatca ttgtaataaa cgaaggaata
      781 gtaatgaaaa aggtatttgc aaaatctctt ctggtcgcag cgatgttttc tgttgcaggc
      841 tccgcattgg cagtacaaaa ggatattact gtaacggcca acgttgatgc cgctctggat
      901 atgacacaga ccgataacac cgcgttgccg aaagcagtag aaatgcaata tctgccgggg
      961 cagggtctgc aatcttacca gttgatgacc aaaatctggt ctaacgacgt taccaaagat
     1021 gtaaaaatgc agttagtttc cccggcgcaa ctggttcaaa gcctggatgc tagcaagatt
     1081 gttccgctga cagtaacctg gggcggtgaa gaaattaaag ctgatgcggc aaccactttt
     1141 accgcaacca aaatctttgc ttccgatgcg ttaactaacg gttctctggc taaaaacctg
     1201 atgtttgctc aaaccaccaa aggtgttctg gagacaggca tctaccgtgg tgtagtaagc
     1261 atttatctgt ctcaggatat ctaatccaga taacagaatt aacacgtaac aaagggaggg
     1321 gtagccctcc cttattttaa ctgttttagt atttgttatg gatttcagga ttgccatgga
     1381 taaaaaatta ctggctcttt tgatcctggc gagtctcagc ccggcagagg cgacattaac
     1441 cgaaattccc gcagggtttg aggttattgc tcagggacag caggagtata tcgaggttta
     1501 tttttcaggg aaaagtctcg gtaaatatta tgcaatggtt aatcttgata ccgtaacatt
     1561 tcttgatcca gcaagtctat ataacaagct ggaactggat gtagacgatc agaaaatcgc
     1621 gcatatagtg aaagaaaaat tatcgcagcc gctagctcgc cacggtgaat tggcttgcgg
     1681 ttatgtacgt actgactcag ggtgtggttt tctgaatacc gatacgctgg aaataatcta
     1741 taatgatgaa gaaagttcgg caacgttgtt tattaatccg caatggaatt cagctttcga
     1801 tgcgaagtca ttatatttaa atccagacaa aaatactgtt aatgctttta tacatcagca
     1861 agacatcaat gttctggcac aggatgatta ccaatcgttg tctattcagg gaaacggtgc
     1921 gctgggaata acagaaaata gttatattgg tgcacactgg aatttcaacg gttatgatgc
     1981 agatgatgtc agtgacagta atgctgacgt cagcgatctc tattatcgtt atgatttttt
     2041 acgtcgttat tatgtgcagg cgggtcgcat ggacaaccgc acactattta atgcacaagg
     2101 cgggaacttt acctttaact ttctgccact cggtgcaatt gatgggatgc gtatcgggtc
     2161 gacactgagc tatttaaacc aggcgcaaag ccagcaagga accccagtaa tggttctgct
     2221 ttcgcgcaat tctcgtgttg acgcttatcg taatgagcaa cttttgggat cgttttatct
     2281 caatagtggt tcgcaattta ttgataccag ttcctttccg ccaggcagct acagcgtggc
     2341 attaaaagtc tacgaaaata accaactcac ccgcaccgag cttgtgccgt ttaccaaaac
     2401 cggcggtctg actgacggaa atgcgcaatg gttcttacag gcaggtaaaa ctacatcaca
     2461 ggtttctgat gatgaaagtt cagcttatca gctaggagta cgcctgccat tacatccgca
     2521 atatgagctc tacgcagggc tggcgaatgc cgatgatgtg agtgctttcg agttaggtaa
     2581 taactggacg gcagatttag gcggggtggg gaatcttgca atcagcgcca gcgtgttccg
     2641 taacgatgac ggcggcaaag gtgatatgca acaggccaac tggagtaatc cgggatggcc
     2701 gacgttgggc ttttatcgga ccaactctga cggcgatgct tgtacaaccg acagcagaga
     2761 gagctataac gccttaagct gttatgaaag tatttccgcg acggtttcac agaattttgt
     2821 cggctggaat atgatgctgg gttatacccg cacacaaaat aacactgatg atagtttgcg
     2881 ttgggataaa cagcagagct ttgaaaataa ctatcttcgc cagacaacag cgcaaagtat
     2941 ctctgaaact gtacaactta gcgcttcccg cgcttttgtg atgcgtgact ggattttgag
     3001 tacgtcagtc ggtgttttcc atcgtaatga caacggtggc gataacgacg acaacggctt
     3061 gtacttatcg ttttcgttat ctgacacgcc aacgatggat agcaataaca acagccattc
     3121 aacaaatgtg tctacggatt atcgttatag cgaacaggat ggcgatcaaa cgtcatggca
     3181 gttatcgcat accttttata acgattcatt cagccataaa gaacttggcg taaccgtcgg
     3241 aggcctgaac accgatacca taaacagcgc ggttaacggg cgttgggatg ggcaatacgg
     3301 aaatgtctac gctaccgtat ctgatagtta tgaccgtaag aatcatgatc atctttcggc
     3361 ctttacgggc acttatagct ctacgctggc tgtcagttgc tatggcgtta atttgggagc
     3421 cagtggtaca gacgatttgc tgggtgcggt actggtggat gtgaaaggct tctctgaaca
     3481 ggatgaagag agtcaggatc tgcaactcga agcgcgggtg gcaggcagcc gaactttgca
     3541 gcttggtcaa agtgacagtg tgttgttccc ttatcctgga tttcagtctg gttttgttga
     3601 ggttaacgac agtagccagg gcaatcagca ggggacaaca aacatcatta acggtgcagg
     3661 gaatcgtgaa ttaatgttgt tgcctggcaa gctgcgctat cgcgaagtgt ctgccagctt
     3721 taactacaac tatatcggtc gtttattact gccggcagcg gtgaaaaaat tcccgattgt
     3781 ggggctgaac agcgccatgt tactggtagc tgaagatgga ggatttacac ttgaaattaa
     3841 cggcagcgaa aaagagctct atctgctttc cgggcagcaa ttccttaagt gtccactgag
     3901 tgttgtaaag aaacgcgcca gcattcgtta cagcggagat gttacttgta gtgtggtgac
     3961 ttattcacaa ttaccggagt cgattcaggt tcaggcacag ttgaaacagc ctaaattacg
     4021 tggaaacgtt cagacggcgc aaagggaggt tgcaccatga gaaatcgatt gattgcggcg
     4081 atattgggct tgtttggcac gctcattggc gttcaggcag ctcctgacgt gaccagtgaa
     4141 attacgtatg atttggcatc tggtagagcg gattattact tctggaagga tgaagcgtcg
     4201 gcaggaaata atggatatat gtggtatgaa tgttcgtatc ctgacctcca acaaacctgt
     4261 acagctaatg gaaatatatc gacagtacaa atctatttaa ctgaacaacg cagtgggatg
     4321 cgttggccgg taaaactcaa aggatttaaa acagccattg taagtagcga tgaagcgccg
     4381 ccaggatgca aggggggcaa agggcttcag acgaatctta aggattctaa tagatcttca
     4441 tgtacagaag atggtcaaca ttattatata tacgatacaa agtttcttac gctctacctt
     4501 gagcagacag agatgaagaa tttgccgatt ggtggcgtct ggaaggggaa agttaaatta
     4561 cattcgaaca gcccggccca ggactatttc gcaaatatta ccctgaatac gctcgacccc
     4621 aaccatattg acgtgttctt cccggagttc gcccacgcca cgccaagggt gcagttagac
     4681 ttgcatccaa caggaagcgt taacggcagc aactacgcgc aagatctgac catgttggac
     4741 atgtgcctgt acgatggttt taacggtaat gccatcagtt atgaaatcat gctcaaagat
     4801 gaagggcgac cagctgcagg gcgcagagac ggtgacttct ctatctatcg tcaaggaggg
     4861 accaccaccg acgagggaga acgcattgat taccgggtca aaatgtacaa cccggaaacc
     4921 ggtgggcaaa ttgatgtgcg caataatgaa aatatggtct ggaacagcat taacctgaaa
     4981 cgtgtgcgtc cggtggtact gcccggtatt cgctatgccg tgatgtgtgt gccgacaccc
     5041 ttgactctgg cagtcgataa attcagcgtg atggataaac aggccgggta ctacatgggc
     5101 aaattgtcag taatctttac gccttccttg ccaaccatca attgaccccg cagaccagaa
     5161 aaaactaaac aacgaagccg cctgataagg cggcttcgtc attacaactt acgttaaacg
     5221 ttaaacgtta atgttgcggt gcgggatcgt tatgtgtttc ctgcgccacc tcaatcgccg
     5281 gtttgttctg caatacggtc cagataacca gcgcacccag caggtcaaat actgccagaa
     5341 ctgcgaacag cgggctgaag ccgatggtgt cagccagtgc accgacaacc agcgcaaaca
     5401 gcgtacttgc cagccatgcg gacatcccgg ttaaaccgtt cgccgtcgcc acttcgttac
     5461 gaccgaacac gtcagaagag agcgtaatca gcgcgccaga cagtgcctgg tgggcaaaac
     5521 caccgataca cagcagcata attgcgacat acgggttggt gaacagaccg atcatacccg
     5581 ggccaatcat cagcacagca cccagcgtta cgaccatctt acgggaaacg atcaggttca
     5641 caccaaacca acgctggaac agcggcggca ggtaaccacc gaggatacaa ccgaggtcag
     5701 caaacagcat cggcatccag gcgaacatcg cgatctcttt caggttaaag ccgtaaactt
     5761 taaacatgaa cagcgggatc cacgcgttaa aagtacccca ggccggttct gccaggaaac
     5821 gcggcagtgc gataccccag aactgacggt tacgcaggat ctgaccaacg gacattttct
     5881 tcgccgtgct cacctggtgc tgggcttcct gaccattaat aatatagtcg cgttcttcat
     5941 cggtcagatg cttctggtcg cgcggatgtt tatagaaaat cagccatgcc atcgcccaga
     6001 taaagctcaa tgcaccggag atgataaatg ccatctgcca gctgtgcatc acgattgcca
     6061 taccaccagc ggcggcgcaa tcatcgcacc aatcgaagaa cctacgttaa agtagcctac
     6121 tgcgatggaa cgctctttcg ccgggaacca ttcggagctg gctttcagac ccgccgggat
     6181 catcgctgct tccgcggcac cgaccgcacc acgagcaaca gccaggccac cccagctacc
     6241 tgccagcgcg gttacaccac agaacacggc ccacagcaca gcaaacattg cataaccgat
     6301 tttcgtaccc agcacatcca gtacataacc tgctaccggt tgcatgaccg tataagcagc
     6361 agaataggct gcgatgatat aggaatactg ttgggtggag atgtttaact cttccatcag
     6421 agttggcgca gctgccgcca cagtgttacg cgtcaggtaa ccaagcacgg tgccgagcgt
     6481 caccagtgcg atcatatacc aacgtaaccc tttaatttta cgcatgtaaa acctcatcgt
     6541 tttgttatat ccgcaggaag cgcggccatc atcccgccag aggctgaaga tgttagttac
     6601 gaccgggtaa ccgaaaaaaa accggcacgc cccgaacgtt gccatgaaag tttcgtgcta
     6661 attcggcttc cgtaccggtt aacccgattc atctaacaaa tgaatgaatc ggcaatttgc
     6721 attccgtcga cttatgattt gcgacggcag aaagataact tgtcatacaa ctttaaaagg
     6781 tgagagccat cacaaatgtg cgaatatttg tagggacatt acctgacgac agcgaggcca
     6841 gtactggcgc ggcctgcagc gagatttatc actttgagag taattttttt aactacgttt
     6901 attgatctaa ctcacgaaaa tatcttcgga ctctggaaat tggtgtgata actttgtcag
     6961 catcgcacca taagcaagct agctcactcg ttgagaggaa gacgaaaatg actccgttta
     7021 tgactgaaga tttcctgtta gataccgaat ttgcccgccg tctgtatcac gactacgcaa
     7081 aagaccagcc gattttcgat taccattgcc atttgccgcc gcagcagatt gcggaagact
     7141 atcgttttaa aaacctgtat gacatctggc tgaaaggcga tcactacaaa tggcgcgcta
     7201 tgcgtaccaa cggtgtggcc gagcgtctgt gtaccggtga tgcatctgac cgtgaaaaat
     7261 ttgacgcctg ggcggcgact gttccgcaca ctatcggcaa cccgttatac cactggacgc
     7321 accttgaact gcgtcgtccg tttggtatca ctggcaaatt gctttctccg tcaactgccg
     7381 atgaaatctg gaacgaatgt aacgaactgc tggcgcagga taacttctcc gcgcgcggca
     7441 tcatgcagca gatgaacgtg aaaatggtcg gcaccaccga tgacccgatc gattctctgg
     7501 agcatcacgc agagatcgcc aaagacggct ctttcaccat caaagtgctg ccgagctggc
     7561 gtccggacaa agccttcaac atcgaacagg cgacctttaa cgactacatg gcgaagctgg
     7621 gcgaagtttc cgataccgac attcgccgct ttgctgacct gcaaactgcc ttgaccaaac
     7681 gtctggatca cttcgccgct cacggctgta aagtgtctga ccatgcgctg gacgtggtga
     7741 tgtttgctga agcgaacgaa gcggaactgg acagcattct ggcgcgccgt ctggctggcg
     7801 aaaccctgag cgagcacgaa gtggcacagt tcaaaactgc ggtactggtg ttccttggtg
     7861 ccgaatatgc acgtcgcggc tgggtacagc agtaccacat tggcgcgctg cgtaataaca
     7921 acctgcgtca gttcaaactg ctggggccgg atgtaggctt tgactccatc aacgaccgtc
     7981 cgatggcgga agagttgtct aagctgctga gcaagcagaa cgaagaaaac ctgctgccga
     8041 aaaccattct ctactgcctg aacccgcgcg ataacgaagt gctgggcacc atgatcggta
     8101 acttccaggg cgaaggtatg ccgggcaaaa tgcagttcgg ttccggctgg tggtttaacg
     8161 atcagaaaga cggtatggaa cgtcagatga cccaactggc gcagctcggt ctgctgagcc
     8221 gctttgtcgg tatgctgact gacagccgta gcttcctgtc atacacccgt cacgaatact
     8281 tccgccgcat tctgtgccag atgatcggtc gctgggtgga agcgggcgaa gcaccggcgg
     8341 acatcaacct gctgggcgag atggtgaaaa atatttgctt taacaatgcg cgtgactact
     8401 tcgccattga actgaactaa ggtctggggt tgatatgcaa tacatcaaga tccatgcgct
     8461 ggataacgtc gcggtcgctt tagcagattt ggctgaaggc acagaagtca gtgtcgataa
     8521 ccagactgtt acgctgcgcc aggatgttgc tcgtggacat aaatttgcgt taacggatat
     8581 cgcaaaaggg gccaacgtca tcaaatatgg cctgccgatt ggttatgcat tggcggatat
     8641 tgcggcgggg gaacacgtcc acgcccacaa tacgcgcacg aatctgagcg atctggatca
     8701 gtatcgctat caacctgatt ttcaggatct gcctgcgcaa gcggcagatc gtgaagtgca
     8761 gatctatcgt cgcgctaacg gcgatgtcgg ggtgcgtaac gagctgtgga tcctgccaac
     8821 cgtgggctgt gtcaacggca tcgcgcggca aatccagaac cgtttcctga aagagaccaa
     8881 caacgccgaa ggtaccgacg gcgtgttcct cttcagccac acctacggct gctcacaact
     8941 gggcgacgat cacatcaaca cccgcaccat gctgcaaaac atggtgcgcc acccgaacgc
     9001 gggcgcagtg ctggtgattg gtctgggctg tgaaaacaac caggttgccg cattccgtga
     9061 aacgttgggc gatatcgatc ctgaacgcgt tcatttcatg atctgccaac agcaggatga
     9121 tgagatcgaa gccgggatcg agcatttgca tcagctgtat aacgtgatgc gcaacgataa
     9181 acgcgagcca ggcaaactca gcgaactgaa gtttggtctg gagtgcggtg gttctgacgg
     9241 tctttctggt attactgcta acccgatgct ggggcgtttc tctgactacg tgattgctaa
     9301 cggcggtact accgtactga ccgaagtgcc ggagatgttt ggcgcagagc agttgctgat
     9361 ggaccattgc cgcgacgaag caacgtttga aaaactggtc accatggtca acgactttaa
     9421 acagtacttt attgcccatg atcagccgat ctacgaaaac ccatcaccgg ggaacaaagc
     9481 gggcggtatc accacgctgg aagacaaatc acttggctgt acccagaaag cgggttccag
     9541 tgtcgtggtt gacgtgctgc gttacggcga gcgtctgaaa acgcctgggc tgaacttgtt
     9601 aagtgcgccg ggtaacgatg ccgtagcgac cagcgccctg gcgggtgcgg gctgccatat
     9661 ggtgctgttc agtactggtc gcggcacgcc atacggtgga tttgtgccga cggtgaaaat
     9721 cgccaccaac agtgaactgg cggcgaagaa aaaacactgg atcgactttg acgcgggtca
     9781 gctgatccac ggtaaagcga tgccgcagtt gctggaagaa tttatcgaca ccatcgttga
     9841 gtttgccaac ggtaagcaaa cttgcaacga gcgtaacgac ttccgcgaac tggcgatctt
     9901 caaaagcggc gtaacgctat aaagtcgaaa tgagttgtta ataaaaaacg gcgtttcata
     9961 atgtgaagcg ccgtttgctt ttccatacct gataaggatt tatcatgacc gcgtattggc
    10021 tggcccaggg cgtgggtgtc atcgcctttc tgattggtat cacaacattt ttcaatcgtg
    10081 acgaacgtcg cttcaaaaag cagctttcgg tctatagcgc cgttattggc gtacattttt
    10141 ttcttctggg cacctatccc gctggtgcca gtgccatcct taatgccatt cgtacattga
    10201 ttaccttacg cacgcgcagc ttatgggtaa tggcgatttt tattgtgctg actggcggaa
    10261 ttggcctcgc gaagttccat catcctgtcg aactattgcc ggttatcggt acgattgtca
    10321 gtacctgggc gctgttccgc tgtaaagggc tcaccatgcg ctgcgtgatg tggttttcaa
    10381 cgtgttgctg ggtgattcac aacttctggg cggggtcgat aggcggcaca atgattgagg
    10441 gtagttttct gcttatgaat ggcttgaata tcattcgttt ctggcggatg cagaaaaggg
    10501 gaattgatcc gtttaaagta gagaaaaccc ctcccgccgt agacgaaagg ggttaaacaa
    10561 ttaattacgc agggcgctat ttgccagacg atcgtcttcc gcctggcaag ctgccgcagt
    10621 gaacagcacg tcagttgaag agttcagcgc ggtttcgcaa gagtcctgca atacgccgat
    10681 gataaagccg acggcaacca cctgcatggc gatatcgttc gaaataccga acatattaca
    10741 ggccagcggg atcagcagca gagaaccccc cgccacgccg gatgcgccac aggcacacag
    10801 agaagccacc acgctcaaca gcagcgccgt gggcagatcg accgggatac ccagcgtatt
    10861 aaccgcagcc agcgtcaaca cggtaatggt gattgctgcg cccgccatat tgatggtggc
    10921 acccagcgga atagaaacgg aataggtatc gcgatccaga ttcagctttt cacacagtgc
    10981 catattcacc ggaatgttcg ccgcagagct gcgggtgaag aaggcataca cgccgctttc
    11041 gcgcaggcac agcagcacca gcgggaacgg gttacgacga attttccacc acaccagcaa
    11101 tgggttaacc accagcgcca ccagtaacat acagccaacc agcacgacca gcagttgcgc
    11161 gtagccccac agtgtggaga aaccggtggt tgccagggta gaagaaacca gaccaaaaat
    11221 accaatcggt gcgaagcgaa tgaccagttt caccataaag gtgacggcat tcgacatatc
    11281 gttgaccagg tttttggtgg tctcgttacc gtgacgcagt gcgaaaccga ggccaattgc
    11341 ccacaccagg atcccgatgt agttaccttt cagcagcgcg tcgatggggt tggaaaccat
    11401 gctcattacc agcccgcgca tcacttctac aatgcctgac ggtggcgaaa tatcacccgc
    11461 gctactggat aagtgcaggg tagaagggaa ggcgaagctg aaaacgactg cggccagagc
    11521 agcagagaag gtacccagca gatagaggaa caaaataggg cggatattgg ttttctgccc
    11581 gtgctggtgg ttagcaatag atgccatcac cagcatcaac accaggatgg gggcaacggc
    11641 tttcagtgcg ccgacgaaca aagtacctaa cagaccaaca gcttccgccg cgggttttga
    11701 tatccatgcc agaagaatcc ccagaacaag gccgaccagg atttgtttta ccaggctgcc
    11761 atgagccaga cgccggaata gccccggtga acgttgcgta gtcatttttc gatcctttca
    11821 ttgtgttatc gcacatccct ggtgcgttct gtcgacgcat ctttcaggat gtaacaaagt
    11881 gtttgcaagg tcgagtataa ggaaagttac agcttcggga agcgaaaaat gctggatttt
    11941 aaagagaagt catattttga aacttaatgg ttacatttgt ttgacataaa cagagggaag
    12001 gactggctac taaggctgat tagataacat atgggcaaaa ctatttggaa tatgagaacg
    12061 gttgcgctcc tttaatgtgc tatgccatat aaagtcactg gcgcagggag cgcgcagggg
    12121 gcggccaatc gccgccgccc cctgcacccc caggctctgg cgaacaaaat cgccgctgcg
    12181 cgattccctc agcttatccc ttgcggctac cgggtcgggc gcaagggaac gtccctgtaa
    12241 gatgcgccct cgccgcgcat ccatgcgcgt cgccccggcc tccagggaac gcttcggcga
    12301 ttttgacgcc accaacaccc gtgcggtctt tttgtaaaga gctacaagcc gattacataa
    12361 ttaatagctc acctttgtct ggaatgagtt atctctagga atggcttaat atacagcgaa
    12421 tcattctccc cgctgcttat catgccgata attcacccag gcgttgataa taaacgtcat
    12481 caccagaatg ccaaacacca cgcccagcga gacggcgatt ggaatatggt agaagtcgac
    12541 aatcagcatc ttgataccga taaacaccag aatcaccgcc aggccatatt tgagcatcga
    12601 gaaacgctct gctacgcccg ccagcaggaa atacatcgca cgcaggccga ggatcgcaaa
    12661 caggtttgag gtcagcacaa tgaacgggtc agtggtcacg gcgaagatag ccggaatgct
    12721 atccacggcg aaaatcacgt cgctcaattc caccagaatc agtaccagca tcagcggtgt
    12781 ggcgtacagc aatccattct tacgcacaaa gaaatgctcg ttgtcgatgg tgtcggtcat
    12841 gcgcaaatga ccgcgtagcc agcgcaccag cggcttgtcg ccaatgcctg attcatcttc
    12901 atgggcgagc gccatcttca cgccggtaaa cagcaggaag gcaccgaaga tatacaggat
    12961 ccagtcgaac tgcgaaatta gccagctgcc agtgaagatc atgatggtac gcagaacaat
    13021 cgccccgagc acgccataca ccagcacgcg gcgttgtaat gccgccggaa cagagaaata
    13081 gctgaacaac atcagccaga caaagacgtt atcgaccgcc agcgatttct caatcagata
    13141 acccgtgaga aaggccagtg cctgtggatc ggcaacggcg cgaccttcag tttgcaccag
    13201 ataccaccag aaagcggcgt taaacagtaa cgacagcgtc acccagacca gcgaccaggc
    13261 cgcagcctgt ttcatggtca tggcatgtgc cccacgacgc ccctgcaaca acaggtcgat
    13321 agccagcata atggcgacaa caacagcgaa tccgccccat agcaacggcg tgccgacagt
    13381 attcatagaa gttccttaca cataaaaaaa cggccaacgt cggaagacgt cagccgctgc
    13441 tttttatgca tagacctcgc cttccggcaa ggtctcactt acaacaaaaa aggaatacgt
    13501 ctgcgtattc ctttcgggtt gcccggtgac cggatgtggt ttttcacaca tcgtaatgac
    13561 gatcgaccgg caatgaagtt actccccttt gcaggtaaca aagtatgaca gaccattcag
    13621 ccagtcaatg gtctgttaca taccttttac actgttttac tttggcaatt atagttttac
    13681 gctatctgcc ggaaaaatca ccccagtctg gcggcggatc tcagtcagca gtttggcggt
    13741 gatgcgactg accgccagcc ctggatgatc caccagatgc tcatccacca gggtagcaaa
    13801 cagctctgct tcgtagagca tggtattaat atgctgcggc tgggtgagat cctgcatctg
    13861 gctgccacgc ggcacgaagc acactttctg gcattcagac agtttttcaa tcaccagcga
    13921 tcccgcttcg ccctgaatct cgctttccag gacagaatca ctgacctttg agtgctgcaa
    13981 cgtaacgctg aaatcaccgt aatccatcac caccacgccg tgggcatcta cgccgcttgc
    14041 caacaaactg gcggttgcct gcacgctttt cggttcaccg aataatgcca ccgccgacgc
    14101 caggcagtaa aagccgatat ccataattga accgttagag aatgccggat taaaggtgtt
    14161 gggattctca ccatccaggt aacgttgata ccgcgaggaa tactggcaat agttgaaaaa
    14221 gactttacgc aatttgccga ctttcggcag cgcctggcgc agcaaatgaa agttcggcag
    14281 gctggcggtt ttaaatgcct caaacagcac cacctgattt tcccgcgcac aggcaatggc
    14341 ggcatccact tccgccagat tcgacgccag tggtttctcg caaatcacat gaattttatg
    14401 gctaaggaaa agttgtgtct gggaaaagtg cagggcattc gggctggcaa tatacaccgc
    14461 gtcaatggca tcgctttccg ccatcgcttc cagcgaggta aacagatgct cgacagaaaa
    14521 atcattggcg aagtgctggg cctgttcaag gctgcgggaa tatacggcgg ttaacttgta
    14581 tttaccgctc tcatgggcgg cctcgacgaa ctggcgagtg atccagttcg taccaatcac
    14641 agcgaaacgt atcataacgc gtggatactc cataaaggga ttctgtcgtc aatgtagcac
    14701 gcctttttca tcttcgacgt gcgcagatac gcattattct ttcgccggac agccatgctc
    14761 actaaacggc ccaatcatga ccgatggccc gagatatttc ggctggcgat ggaggatttt
    14821 ggcgtcaatc accgcgctga cacgagcgag tttctcccgc agttgagtat aaaaaccgtg
    14881 cttcatatta aggtctggcg cgttaaaccc gatagcgtcg atgccgtact gttttgccag
    14941 ccagatggcg cgttggttat gaaattcttg cgagataatg gtgatatggt tttccccaaa
    15001 cacctttttg gcacgcacca ctgaatcaag cgttgagaat ccggcatagt cgcagaagat
    15061 cactttcgcc ggtacgcctt tagcgatcaa agcctgctgc atacctgagg cttcatcgta
    15121 atttttacgt ccgttatcgc cactgactaa cagccatttc acttttccga cgtggtacaa
    15181 cgctgctgcg gtatcaatac gtcgggtaaa gtagcgatta cccggcctcg cccccagcaa
    15241 taaacccacg ttacgcgccg gaactgcgtt gacatcgctc caggtcagct gcttgctggc
    15301 gttgaccatt acgcgatcgg cgataaaaat ggtcgctcct gccacaagta acagtaagca
    15361 ggtaatcttt agagtccggc gagaaaaaca aatacggaga agaaggcggg gctgcatggt
    15421 cgttatcctt aacgaaaggg gggaggtcga ttttaggcgc taaagaaaga aaagataacg
    15481 cctgacatca ttttcagatt ttgtcctgcc caagcgacaa ctgcgttagc cacagccggg
    15541 tgtcgaactc aagctggtgg tactgcggct ccatatggca gcacaactgg taaaacgctt
    15601 tgttatgctc tttctccttc aggtgcgcca gttcgtgcac cacgatcatg cgtaaaaacg
    15661 gttccggcgc gttgcgaaac acagtagcga cgcggatctc tgcttttgct ttcagcttgc
    15721 cgccctgcac acgagaaaca gcggtatgta atccgagcgc gtttttcagt acgtggatct
    15781 tattgtcata catcacttta gtgatcggcg gggcattgcg cagaaactga ttttttagat
    15841 cctgggtata ttgccagagg gctttatcgg tggcgtagtc gtgtgttccc ggatagcgtt
    15901 ttgccagcac atcgcccaga cgctgttcgt taatcagcgt tcgcacctgg gaaagtagct
    15961 gctcgggata accctgaaga taagtaagat tgctcataac cgcccacgaa aaagagaaaa
    16021 agggtatact cacgcaccct tttcaggggc aacgtcgaaa ttttatcatt caggagggcc
    16081 gatgagccac ttagacaacg gtttccgttc actgacacta caacgttttc cggcgacgga
    16141 tgacgttaac ccgctacagg cgtgggaagc ggcggatgaa tatttgctgc aacagttgga
    16201 cgacacagaa atccgcggcc cggtgttgat cctgaatgat gcctttggtg cgttaagctg
    16261 cgcgctggcg gaacataagc cgtacagcat tggcgactca tacatcagtg aactggcgac
    16321 gcgcgagaat ttacgcctca acgggattga tgagtcgagc gtgaagtttc tcgacagcac
    16381 cgccgactac ccgcaacagc cgggtgtggt gctgatcaaa gtgccgaaaa cactggcatt
    16441 gctggaacag caactgcgtg cgctgcgcaa agtggtcacg tcggatacac gtattattgc
    16501 cggtgccaaa gcccgtgaca ttcacacttc cacgctggag ctgttcgaga aagtgctcgg
    16561 cccgaccacc actacgctgg catggaaaaa agcgcgcctg attaattgca ctttcaatga
    16621 accgccgctg gccgatgcgc cgcagaccgt tagctggaag ctggaaggta ctgactggac
    16681 tatccacaac catgcgaatg tcttctcccg caccgggctg gatattggcg cacgcttctt
    16741 tatgcaacat ctgccagaga atctcgaagg tgagattgtc gatctcggtt gcggtaatgg
    16801 cgttattggt ctgacgctgc ttgataaaaa ccctcaggcg aaagtggtgt ttgtcgatga
    16861 atcgccgatg gcggttgctt ccagccgttt gaacgttgaa accaacatgc cagaggcgtt
    16921 agatcgcagc gagtttatga tcaacaacgc gctctccggc gtggagcctt tccgctttaa
    16981 tgctgtgctc tgcaacccgc cgtttcacca acaacatgcg ctgaccgata acgtcgcctg
    17041 ggagatgttc caccatgccc gccgctgcct gaaaatcaac ggcgagctgt atattgttgc
    17101 caaccgtcac ctggattact tccataaact gaagaagatt ttcggcaact gcaccaccat
    17161 cgccacgaat aataaatttg tggtgctgaa agcagtgaag ctggggcgtc gtcggtaaga
    17221 ttgggagtta cctgccggat gcggcgttaa cgccttatcc ggcctacgtt cagcacgtta
    17281 ctcccaggcc ggataagacg cggcaagcgt cgcatccggc aatgtctgcc cagttctgga
    17341 atcgcgcttc cataaaacaa aaaatccctc tcaccatcct ctacattctc ttgtttagcg
    17401 tttttctacg tttattcttc cgtcacacag ataaattcat ccgttgccaa tctgtcaacg
    17461 gatgttatca tattgccaat ttgtcaacgg acgtgatgaa gaatgcacct gataactcaa
    17521 aaagcattga aagatgctgc ggaaaaatac ccgcaacata aaacggagtt ggtggctctg
    17581 gggaacacga ttgctaaggg atatttcaaa aaacctgagt cattaaaagc agtattccca
    17641 tctctggata acttcaaata tctggataag cattatgttt tcaatgttgg gggcaatgaa
    17701 ttacgtgttg tagcaatggt cttttttgaa tcgcaaaagt gctacatacg tgaagttatg
    17761 acgcataaag aatacgattt ctttaccgct gttcatcgta ctaaggggaa aaaatgattg
    17821 cgattgccga catcttgcaa gcaggagaaa agctaactgc ggtggcacct tttctggcgg
    17881 gtattcagaa cgaggaacaa tacacccagg cgctggaact ggtagatcat ctgctgctca
    17941 acgatcctga aaaccccttg ctggatctgg tgtgtgccaa aataaccgcg tgggaagaat
    18001 cagcgcccga atttgcggaa tttaatgcca tggctcaagc catgcctggc ggtatagccg
    18061 tgattcgtac ccttatggat caatatggtt taacccttgc cgatctgccg gaaattggca
    18121 gtaaatctat ggtgtcacgc gttttgagcg ggaagaggaa attaacgctg gaacacgcta
    18181 aaaaattggc aacgcgattc ggcatttctc ccgccttgtt tattgattaa cgcgacgtgc
    18241 cggatgcgtt gcatccggcg cttaaaataa cgatttaaat ctccagcgcc agccgtgttc
    18301 cctgggcaat tgcccgtcgt gcgtccagct ccatagccac atcgcagccg ccaattaaat
    18361 gcacagtttt cccgctatca atcagcggtt gcgccagcgc gcggtttggc tcttgccctg
    18421 cgcagatcac cacattgtcc actgctaata cctgcgtttc gccgttgatc accacatgca
    18481 gcccgtcatc gtcaatcttc tgataactta cgcctgggat cattttcaca ccccgcgaga
    18541 gcagggtggt gcgatggatc cagccggtgg ttttgcctaa cccctgtccc ggtttgctgg
    18601 ctttgcgctg gagcatcaca atttgccgtg ggctacgcgg gatctgcatt ccctgcgggc
    18661 ttaagccacc agcctgttgt aggctgctgt cgatccccca ctcattacag aacctggcga
    18721 tattctggct ggtggattcg cccggctgac ttaaatacat cgccgtatca aaaccaatcc
    18781 cgccacaacc gatgatggca accttgttgc ctaccggcgc tttgtcgcgc agtacattga
    18841 gataactcaa taccttcgga tgatcgatcc cgtcgatagg cggaacgcgc ggcacgatcc
    18901 cactggcgag gatcgtttca tcgaacgcct gtaactgatc cgccgtcacg gtgtgattga
    18961 gtttcagcgt cacgcccgtc acttcgatca tccggtggta atagcgcagc gtttcgtaaa
    19021 actcctcttt gccggggatc tgtttggcga tattaaactg cccgccgatc tcgctgtggg
    19081 catcaaacaa tgttacctga tgcccacgcg ccgccgcgtt aatggcaaac gccagcccag
    19141 caggtcccgc accgaccacc gccagatttt ttttctgcac ggcaggaagg attggcattt
    19201 tggtttcgtg gcaggcgcga ggattcacca ggcacgaggt gactttgcca acgaagatct
    19261 gatcgaggca ggcctgattg cagccaatgc aggtgttgat ctcatcggct cgccccgatt
    19321 gcgcttttga cagcagttcc gcatcagcaa gaaacggtcg tgccatcgat accatatcgg
    19381 catcgccgcg cgagagaata tcgtcggcaa cctgcggatc gttaatccgg ttggtggtta
    19441 ccagcggcag cgagacgtgg cctttcagtt tgcgcgtgac ccagctaaat gcgccgcgcg
    19501 gcacgggcgt ggcaatggtc ggaatacgtg cttcatgcca gccaatgccg gtgttgataa
    19561 tggtcgcgcc cgccgcttca atggcctgcg ccagctctac cgtttcggca aaagtcccgc
    19621 cgtcttcgac caggtcgagc atcgacagtc ggtagataat aatgaagtcg ttgccgacgc
    19681 gttcgcgcac cgcacgcact acttctacgg caaatcgcat ccggttgcgg taatcgccgc
    19741 cccactggtc actacgctga ttggtgcgca gcgtcagaaa ttcgttgatc aaataccctt
    19801 cggaccccat tacctctaca ccgtcgtatc ctgcctcccg tgccagttgc gcgcagtggg
    19861 cgaaatcgtc gatcagttgc aggatctctt catgggtgag ttcgtgagga acgaaacggt
    19921 tgatgggggc ctgcaatgcg gagggagcga ccagatgcgg ttggtagctg tagcgcccgg
    19981 tatgcaaaat ttgcagggct attttgccgc cttcctgatg taccgcttcg gtaatggtgc
    20041 gatggtgtgg gatctggctg gcgtcgttga gcattgcgcc accttccatg ccaacgcctg
    20101 ttaaatctgg tgcaatgccg ccgctgacaa tcagcgccac gccgtgacgg gcgcgttcgg
    20161 cataaaacgc tgccagccgc tcggcaccgt ccgggtattc ctccagcccg gtgtgcattg
    20221 agcccatcaa cacgcggttt tttaacgtgg taaaacctaa atccagcggg gcgaacagcg
    20281 acgggtagct cataaaattg tccagtatgt aaaataattg ttatgtggtc ggatgagttc
    20341 taatttagcc gtcgatggag aaaagggaaa aggggagtgc ggggtttgtg atgggattca
    20401 aaaagaatac gcccggtggt aacgccggat gcaacgcttg ccgcgtttta tccggcctgg
    20461 gggattgcac atgtagggcg gataaggcgt tcacgccgca tccgccagcc gttgcaaatt
    20521 actgcttacg gaaaaaatcg ttatacaaca tatacaaatg agcggcactc caggagaaat
    20581 ttggtgcgcc ttgctgtgcg ccagtcagcg ggttgtaatt ctcctgaatt gggccatcgg
    20641 cggttaatcc tttggcgtgc cggaagaacg tatccgccag cttcagggca tcatcacgat
    20701 aaccgtaacg ctccatccct ttcagaccaa accagaactg atccacccat acgcgcccac
    20761 gccagtagat atcagcgcca aaagccggat tggttaacgc cgccgttccc agcgggacaa
    20821 aggtgttgaa ctctttagga tcgagcatca ccttcaccac cgcgtcggcg ttggcctgcg
    20881 ttgccgcacc gttaaacagc ggcgaccagc cttccggccc tttaccgcgc tcaacaatcg
    20941 gtttgcccgc gcagccgttc gccagtggtt tatcttcaat acgtacgtca tagtagaact
    21001 gggtagtcgg gtcgaacata caggtgttga tgtagtccgc gagctgctgt gccaactggc
    21061 gatagcgttt ggcctcttct ggcttaccga ggatggttgc catctccgcc agataatggt
    21121 tatcgctgta catatagctg gcctgatcca ccgactcctg caatagcgag tagcccagca
    21181 acgttccgtc ctgactgcgg ttttcggcga atttcaccgt ccagtcgcta cgtttgccgc
    21241 cattagcgac atatttatcc agctgttctt tgtcgataaa cccaaagacg gcggcatcat
    21301 cacgccccga ttcccacgat gcagcaacct gtgccggaat ttccagactg tcgtacaggc
    21361 ctttctccac cacgcgggcg tagttgttca gcccagactg cgtctcttct ttatcgcctt
    21421 ttttcaccgt aaacagcatc tcgccgctct cagtgttgtg ggctttgtcg cgggtcgcgc
    21481 catattccgg cacgccgttg ccgttgtgat cgcggttacg taaccaccag tcgtgatagg
    21541 ccaccagttt cgggtacatc tctgccaacc aggctttatc ctgggtgacg ttgtacactt
    21601 ccatcaccga ccaggcggca aggctgggtt tggtattacg ttcgttccag ttgccgccat
    21661 cgccaccacg ctcggggcta aggttccacg ctatcaggtc ggggacaaag cccacatcct
    21721 gcggacgcac gctatcgcca ggctggatct gccaggagaa gaccgcgcgg atattctctt
    21781 tggcgatgtc cggattgaaa tgcgccatcg caaacgcctg cttccaggta tcccacggcc
    21841 aggtctgatt gccggagaac cagcgcccgg tcaccgacgg tgtaacggtg ttaaatttca
    21901 ccgcaccgcc cggtgagagc cagttaccgt tgagcgtttc gatcgctttc accgcgacgc
    21961 gcgtctgttc cggcgtcgca tccggattgg ttaacccttt cttcaggtat tcttcccagc
    22021 gttgctgcga ggcggtgaga taaaacgccg gacgcgccag aatatcgcgg atctgcattt
    22081 gctctttgct aacttcctga gcggtcagca gatgggaata ggtggtgtag agcgtggtcg
    22141 aaccgttgat atgcgcctta ctggtaaagc gattaccgtt gatttcagtc tgcaccggca
    22201 gggatttatg cacctgatat tccgattcgc cggaggtcag cagatcccag gtggcgcgca
    22261 ctttgccaaa ggtgactttc aggccatcac gggtggcgct gattttgcgc tgatagtcag
    22321 ggtattcgcc agcaatggtt ttatcggaaa gcggtttccc ttctttcgct tccagttttt
    22381 ccagcagttc gccgtcccac accagatcca gcggtttatt gctggtgatt ttggtttcca
    22441 gtagtgacgt gcgcggcgtg gcgaagcgca gagtcatttc gacttgcaca tcttttgctg
    22501 tcagtttttg caccagcgca ccgggaatac tgtatgcctc cagcgtaaag tcgactttct
    22561 tgccgtcctg ccagacggtc aggcggtcga aattgctggc cataaagttg atgtactctt
    22621 ccgtcagcag cgcaacgccc ggaaagccgc ccatggtgtt agggccgtct ggcaacagat
    22681 gaccatgcca ggctccgaga tcgaaaaacg gattaaaacg ctggtgatcg tcgtaatcgt
    22741 aatccttcat gtactgcggc gcgccagtac ggttaatcac gtttttataa ttgtcggcgt
    22801 tagcggcatg ggcggaaaaa cttatcagca gagcgcaggt tactggcgtt aaaatagttt
    22861 ttattttcat gaaattacag tccttattac cagtagaaat attgcatcag gaagatttgg
    22921 tcgctgggat cgtgagaggc atttttcatg tagaagcggc tatcaaaagc cgtggcgaaa
    22981 cgtccgccgc cgtattcgta ccagatacca aactgcatga aagaggtggt ttcatcgtca
    23041 gcgtggtcag ggcgatgttt ggcgtaggtg taagcggtgg agagataaac gccctttaac
    23101 gcttcataca tggcgctcac catcatccca ctttcacttc ccggtacgtc cagcccgtcg
    23161 gcgcgtccgg tatggtgcca ggcgcgagcg gcaaagttag gagttagcag gcccgtgaga
    23221 tagagctgtc ctgtgccgtc ggtgatttcc agaccgttca tccaggtgat gccttcggca
    23281 aggtcatact ggatgtaacc gttcatcatt gccggatagg tgtatttgtc gtcatagcga
    23341 tcgtatttgc caaaatgtag ccaggctttg ctttcatcat gatgtccggc aggagtcgcg
    23401 gtgacgctat aacgaaagtt gccgcttaag ttctgtacct tcagcgcagt cataatgtcg
    23461 cgggtattgg gaatgacgta gcccagatcg ggggtgaaat cgccccacca ttgcaggtca
    23521 tccagcgagg aatcctgacg cagactgagc atgatttcgg tgccatcatt ggtacgataa
    23581 ccgccataga aacggttaat tccgccctcg aaaccacccc agctatggtc tggcacccag
    23641 gcattgccgt cgtggtcggc ctgcacggtc cagccttcgc cgtaaatcag gccgaaccag
    23701 gagccgtact tcatctccag gccaccttca atataggtac cgtcgctgta tttatgttta
    23761 ttctcaccct ctaaatccat atatccacca ccccctaatc cctaattcgc cgtaaaaacg
    23821 aaatgaagga gaagatgtcg aggtaattgc gggtgttttt gtgtcgtcat gttctgccgg
    23881 acgtgcttct tctcctgccg cagaaaaggc gtagcaaaac ggcagagcaa gtaatgctct
    23941 gcatggtgca gtgattaatt tcattgtgtt aatccatgag ctaaagaaaa agtacagcct
    24001 tttatcgttg taattataaa aatataaagg cagaaactat tttccctctc ctcatccagg
    24061 aagagggaaa atgcctgctc attttttatt attctgctta atcaacattg ctggctggcg
    24121 tcgcttcaat atggcgctct tcagccgtta atccctttat atatttactg tatttccacc
    24181 acgcaaagcc aaggaagata acaataccgc cgacgttata aaaaatgatg gtcagaatat
    24241 tcgcgccagt cgggaatgtc gaagcgacaa accctacggc aaatatcgca atcagcattg
    24301 aaacaacgat aataccggtg cggcgggagc ccatacggaa atcgcgtggc aggtgatcta
    24361 atttggcgcg taaattcagg taagccagca tgatgaataa cggcggaagc atggacgctg
//
LOCUS       DAZPLI010000052        23670 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZPLI010000052 DAZPLI010000000
VERSION     DAZPLI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23670
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.052"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..163)
                     /locus_tag="ACHUU3_001987"
     CDS             complement(<1..163)
                     /locus_tag="ACHUU3_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR2082060.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFA"
     gene            complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHUU3_001988"
     CDS             complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHUU3_001988"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR2082061.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1684..3492
                     /gene="frdA"
                     /locus_tag="ACHUU3_001989"
     CDS             1684..3492
                     /gene="frdA"
                     /locus_tag="ACHUU3_001989"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR2082062.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3485..4219
                     /gene="frdB"
                     /locus_tag="ACHUU3_001990"
     CDS             3485..4219
                     /gene="frdB"
                     /locus_tag="ACHUU3_001990"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR2082063.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4230..4625
                     /gene="frdC"
                     /locus_tag="ACHUU3_001991"
     CDS             4230..4625
                     /gene="frdC"
                     /locus_tag="ACHUU3_001991"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR2082064.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4636..4995
                     /gene="frdD"
                     /locus_tag="ACHUU3_001992"
     CDS             4636..4995
                     /gene="frdD"
                     /locus_tag="ACHUU3_001992"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR2082065.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5058..6191
                     /locus_tag="ACHUU3_001993"
     CDS             5058..6191
                     /locus_tag="ACHUU3_001993"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR2082066.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6280..6813
                     /gene="blc"
                     /locus_tag="ACHUU3_001994"
     CDS             6280..6813
                     /gene="blc"
                     /locus_tag="ACHUU3_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR2082067.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHUU3_001995"
     CDS             complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHUU3_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR2082068.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHUU3_001996"
     CDS             complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHUU3_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR2082069.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHUU3_001997"
     CDS             complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHUU3_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR2082070.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHUU3_001998"
     CDS             complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHUU3_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR2082071.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8344..9372
                     /gene="epmB"
                     /locus_tag="ACHUU3_001999"
     CDS             8344..9372
                     /gene="epmB"
                     /locus_tag="ACHUU3_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR2082072.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9762..10631
                     /locus_tag="ACHUU3_002000"
     CDS             9762..10631
                     /locus_tag="ACHUU3_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR2082073.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLNAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGTVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(10834..11187)
                     /locus_tag="ACHUU3_002001"
     CDS             complement(10834..11187)
                     /locus_tag="ACHUU3_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR2082074.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAVMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11325..12971)
                     /gene="groL"
                     /locus_tag="ACHUU3_002002"
     CDS             complement(11325..12971)
                     /gene="groL"
                     /locus_tag="ACHUU3_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR2082075.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13015..13308)
                     /locus_tag="ACHUU3_002003"
     CDS             complement(13015..13308)
                     /locus_tag="ACHUU3_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR2082076.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13584..14840
                     /gene="yjeH"
                     /locus_tag="ACHUU3_002004"
     CDS             13584..14840
                     /gene="yjeH"
                     /locus_tag="ACHUU3_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR2082077.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(14862..15332)
                     /locus_tag="ACHUU3_002005"
     CDS             complement(14862..15332)
                     /locus_tag="ACHUU3_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR2082078.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            15669..17105
                     /gene="aspA"
                     /locus_tag="ACHUU3_002006"
     CDS             15669..17105
                     /gene="aspA"
                     /locus_tag="ACHUU3_002006"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR2082079.1"
                     /translation="MSNNIRIEEDLLGTKEVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17223..18524
                     /gene="dcuA"
                     /locus_tag="ACHUU3_002007"
     CDS             17223..18524
                     /gene="dcuA"
                     /locus_tag="ACHUU3_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR2082080.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            18640..18978
                     /gene="cutA"
                     /locus_tag="ACHUU3_002008"
     CDS             18640..18978
                     /gene="cutA"
                     /locus_tag="ACHUU3_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR2082081.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            18954..20651
                     /gene="dsbD"
                     /locus_tag="ACHUU3_002009"
     CDS             18954..20651
                     /gene="dsbD"
                     /locus_tag="ACHUU3_002009"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR2082082.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            20688..21263
                     /locus_tag="ACHUU3_002010"
     CDS             20688..21263
                     /locus_tag="ACHUU3_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR2082083.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            21370..21445
                     /locus_tag="ACHUU3_002011"
     tRNA            21370..21445
                     /locus_tag="ACHUU3_002011"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21403..21405,aa:Phe,seq:gaa)
     gene            21688..21802
                     /locus_tag="ACHUU3_002012"
                     /pseudo
     CDS             21688..21802
                     /locus_tag="ACHUU3_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            22062..22361
                     /locus_tag="ACHUU3_002013"
     CDS             22062..22361
                     /locus_tag="ACHUU3_002013"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFR2082084.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYK"
     gene            complement(22352..22477)
                     /gene="laoB"
                     /locus_tag="ACHUU3_002014"
     CDS             complement(22352..22477)
                     /gene="laoB"
                     /locus_tag="ACHUU3_002014"
                     /inference="COORDINATES: protein motif:HMM:NF038068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arginine responsive protein LaoB"
                     /protein_id="HFR2082085.1"
                     /translation="MVRLWEGEFVSGTASGIGGGEDSIISSPSSSLYQITGTIHL"
     gene            22544..23599
                     /gene="cadC"
                     /locus_tag="ACHUU3_002015"
     CDS             22544..23599
                     /gene="cadC"
                     /locus_tag="ACHUU3_002015"
                     /inference="COORDINATES: protein motif:HMM:NF007540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFR2082086.1"
                     /translation="MVFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVN
                     KSCNSWSSPYQLSYAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVN
                     QRQYRAQQCFMSVKLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQR
                     MQETLQQILPHRGALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEK
                     ALVDIVRHSQHPLDEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKIDESY
                     QAINTGIDLEMSWLNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQ
                     TSVPYVVPYLDKFLASE"
BASE COUNT         5938 a         5998 c         5819 g         5915 t
ORIGIN      
        1 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
       61 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      121 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      181 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      241 aaaaccaata cagaattgat acccggatga tatcaaatat ccagtcttaa attgtggcct
      301 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      361 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      421 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      481 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      541 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      601 cgttaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      661 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      721 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      781 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc
      841 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      901 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      961 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1021 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1081 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1141 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1201 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1261 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg
     1321 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1381 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1441 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1501 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1561 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1621 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1681 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1741 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1801 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1861 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1921 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1981 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2041 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2101 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2161 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2221 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2281 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2341 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2401 tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat
     2461 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2521 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2581 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2641 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2701 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2761 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2821 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     2881 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2941 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3001 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3061 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3121 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3181 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3241 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3301 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg tgatgctgat
     3361 ggcacgactc gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3421 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3481 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3541 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3601 tgcgctgggc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3661 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3721 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3781 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3841 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3901 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3961 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4021 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4081 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4141 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4201 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4261 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4321 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4381 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4441 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4501 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4561 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4621 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4681 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4741 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4801 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4861 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4921 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4981 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5041 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     5101 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5161 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5221 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5281 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5341 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttactgcta
     5401 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     5461 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5521 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     5581 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     5641 tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     5701 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     5761 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     5821 ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct
     5881 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     5941 atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa
     6001 aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     6061 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6121 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     6181 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6241 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6301 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6361 gtgacagtag tgaataattt cgacgctaaa cgctatctcg gtacctggta tgagattgcc
     6421 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagtctgcgt
     6481 gatgacggcg gcctgaatgt catcaataaa ggctataacc ctgacagaga aatgtggcag
     6541 cagagtgaag ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct gaaagtgtca
     6601 ttctttggtc ctttctatgg cggttataac gtcattgcac ttgatcggga ataccgccat
     6661 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6721 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6781 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     6841 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     6901 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     6961 cctaccggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     7021 acagtaataa cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     7081 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     7141 gaattggggc cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     7201 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     7261 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
     7321 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     7381 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     7441 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     7501 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     7561 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     7621 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     7681 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     7741 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     7801 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     7861 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     7921 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     7981 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     8041 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     8101 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     8161 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     8221 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     8281 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     8341 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     8401 gccgatgttg tgaccgatcc tggtgaactt ctgcgtcttt tgaatataga cgcggacgaa
     8461 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     8521 atcgatcgca tggagaaagg caatccggac gatcctattt tgcgtcaggt acttacctcg
     8581 caagatgagt ttgtcgtcgc gtccggattc tccaccgacc cactggaaga acagcacagc
     8641 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     8701 tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
     8761 aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag
     8821 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
     8881 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     8941 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
     9001 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
     9061 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
     9121 gtgaacgata acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg
     9181 atgccgtatt acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt
     9241 gatgacgaag cacggcagat tatgcgtgag ttgctgacat tggtgtcggg atatctggtg
     9301 ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta
     9361 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
     9421 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
     9481 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg
     9541 gaaatttaat ctttgcccgt tgcataacct ttaattattc atatacattg ctgttgttac
     9601 tctttatata gccaacacca ataattaacc tttcttttct gtttatatag agtgagtacg
     9661 gctatttatg ttattttaac aactattact accaagccca tttatctggc ttataaatta
     9721 aaattaatta tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg
     9781 gtgttaatac cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta
     9841 aagaacctcg caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc
     9901 tgattgcgct ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc
     9961 agtatgagca agctcgcgat aaagtcatta agaaaatggc agaaaacatc ccagaaattc
    10021 tggttgatga gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg
    10081 ataatcaagg cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt
    10141 tagtcgtcaa tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata
    10201 atgcggaaat gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat
    10261 acgacgttga ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat
    10321 ggaaacataa cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg
    10381 gcaaagaaca atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa
    10441 tagaggcgat cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg
    10501 tgccatgtca ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg
    10561 atcaatgctt acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac
    10621 aggcgaaata atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg
    10681 ataacgtgat cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc
    10741 tcaagcattc aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca
    10801 ataatacgtt tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc
    10861 gataatctgg ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat
    10921 gccataaatc acgttaccgc ccattacagc cgcctggttg cgcagatcgt ttgctgcgcc
    10981 gcgcatagaa ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt
    11041 cgcagtacca atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg
    11101 accggcagca ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac
    11161 aatccctgct aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac
    11221 tcgcgtcgtc cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac
    11281 atttctgccc gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc
    11341 atgccaccca tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc
    11401 aggtcggtaa ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc
    11461 agagcagaac gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat
    11521 tcttcggttg ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca
    11581 acagacggtt cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc
    11641 agtgcaactt tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta
    11701 gacgctacgc ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca
    11761 cgggtcgcgt gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta
    11821 gcagcaccta ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt
    11881 ttttcacggt cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg
    11941 ccctggattg cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc
    12001 acaacacgtt tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc
    12061 tcttcagaga tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga
    12121 cgatcgccga agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta
    12181 acaaccagag ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg
    12241 cctgctttgg caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg
    12301 tcagccagca ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg
    12361 aagtaaggag acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc
    12421 tgcagaccgg taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct
    12481 tcagcgatca gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca
    12541 atcgctttag agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta
    12601 acggctttgt cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc
    12661 agaccctcag tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca
    12721 gcgtcgttcg ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg
    12781 tcttccagtt cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg
    12841 aaagatttat ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct
    12901 gccagtacgt ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct
    12961 ttagctgcca ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc
    13021 ttcaacaatt gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt
    13081 ctcagatttc acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag
    13141 cggcttcact tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg
    13201 ggtggattta gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac
    13261 ttctttacgc ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc
    13321 tttgagaaag tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt
    13381 gaccagagaa atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg
    13441 atcagcacaa aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat
    13501 cgccgtcata agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt
    13561 tgggcgcaat cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg
    13621 gcattggcct gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag
    13681 ctgcacaggt agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt
    13741 tcccgattgc gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg
    13801 cgcacttcgt cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt
    13861 tatcggtcat tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg
    13921 caatgtttgg ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg
    13981 tgtggtatat cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg
    14041 gacttatcgt cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata
    14101 tccccttccc ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga
    14161 tgttctggtg ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc
    14221 cagagcgtga ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact
    14281 ggggctgtac ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag
    14341 catcgcttcc aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg
    14401 taattggcta tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc
    14461 tggtctggtc gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc
    14521 atatcccgaa taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga
    14581 ttcatgcttt agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta
    14641 tgatttatct gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac
    14701 tggcggtggt tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc
    14761 tctatgcgct gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg
    14821 aaaatggcat aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga
    14881 tcaaggcggt cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca
    14941 ccggtcccgg cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac
    15001 ttcactgtca gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg
    15061 aaaaagcccg gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc
    15121 tccgccgcag ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag
    15181 ccctggttac gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc
    15241 agcaataccc ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa
    15301 aggaaaatgg caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta
    15361 aacccggcta cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa
    15421 atttcaagat gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac
    15481 aaattaataa ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc
    15541 ctcaagcagc atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa
    15601 acattcgaaa ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga
    15661 aggttcacat gtcaaacaac attcgtatcg aagaagatct gttgggtacc aaggaagttc
    15721 cagctgatgc ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca
    15781 acaacaaaat cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg
    15841 cagctatggc aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg
    15901 ccgcatgtga tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct
    15961 accagggcgg cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg
    16021 gtctggaact gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg
    16081 ttaacaaatg tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact
    16141 cttccctgat taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag
    16201 ctgtcgaatt ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga
    16261 tgaccctcgg tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca
    16321 tccaacgtac cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg
    16381 gtctgaacac gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg
    16441 gcttcccatg cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg
    16501 ttatggttca cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc
    16561 tgcgcttgct ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc
    16621 aggcgggctc ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc
    16681 aggtatgctt caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc
    16741 agctgcagtt gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca
    16801 ttctgaccaa cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca
    16861 aagaagtgtg cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt
    16921 tcatcggtca ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg
    16981 tacgtgaagt cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct
    17041 ccgtacagaa tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac
    17101 agtaatcgta cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt
    17161 gtgagcagta acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta
    17221 atatgctagt tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg
    17281 ggggaatagg tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg
    17341 ttaaacccgg taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta
    17401 tttctgccat gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc
    17461 tgcgccgtaa cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa
    17521 tctttgctgg tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga
    17581 aggaacaagg cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg
    17641 cgatcaccgc atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc
    17701 atggcatcag ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc
    17761 tggtgatgtc cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt
    17821 atcgcaagcg tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca
    17881 aatccggtgc aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct
    17941 atgcaatcat caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca
    18001 acgcaatcct gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg
    18061 ataccgacaa catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta
    18121 tcctgggtgt tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag
    18181 ataccgctgg tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg
    18241 cttctgctct gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg
    18301 cactgaacgt ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca
    18361 ttctgccgac ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc
    18421 gtatcggtaa attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc
    18481 tggccgtttg cttcggcttc gtgctgggta gcttcatgct gtaatgacca atcgcggggc
    18541 gttcacgccc cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga
    18601 cctctctctt gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg
    18661 aataccgcgt ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta
    18721 gccgccaaag tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc
    18781 tctctctatt actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa
    18841 actaccgtat ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa
    18901 accccggaac ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc
    18961 aacgcatctt tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg
    19021 acgcgccggg acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc
    19081 aaaaccaaca tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta
    19141 aacagatccg cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg
    19201 tctggcatga agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc
    19261 ccgtaaccat caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg
    19321 ctgatgccgg tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg
    19381 ccaacaacgc agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat
    19441 tgcccttttc cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg
    19501 tgctgccaat gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca
    19561 ctgccagagc attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg
    19621 cgctgggtct ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat
    19681 acgtgctcat tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt
    19741 ttactctaca actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac
    19801 agggcggctc acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac
    19861 catgcaccac cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt
    19921 ggctgggcgg cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa
    19981 ttaccgtctt tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa
    20041 ccgcgtttgg ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg
    20101 atatatgggg attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta
    20161 tcaccagcct acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag
    20221 cggcattggt tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc
    20281 aaactcagac gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc
    20341 tcgttgaagc caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct
    20401 gtaaaaagtt tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca
    20461 cggtcttact tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc
    20521 atcttaatgt ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc
    20581 cacaagcacg cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc
    20641 gccaaccgtg aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag
    20701 atgtactggg agaagccctg aaattattag aattacaagg gattgccaac accacgctgg
    20761 agatggttgc tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca
    20821 aagaggcaat cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc
    20881 gtcagctgat gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg
    20941 cgttatcgga gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta
    21001 cgttttatcc cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg
    21061 cctacgattt cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag
    21121 caaagcagat ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc
    21181 aggcggatgt cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc
    21241 gccagggtgg tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta
    21301 agcagttgaa tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg
    21361 cgccccgttg cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt
    21421 ggttcgattc cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt
    21481 ttcgtttcag gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa
    21541 ccagattctc cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt
    21601 cctgagcgat agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt
    21661 caaatgacgt catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga
    21721 ctgatgagat tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa
    21781 gactaacaat ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac
    21841 acatttttct ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc
    21901 agtaaaatcc tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc
    21961 ttgcattcac caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc
    22021 aattatcatt gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc
    22081 gcgttggcga atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac
    22141 ttacccttga gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag
    22201 tacttagcag ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg
    22261 ttgtgacgca gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc
    22321 ctgtctatat cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt
    22381 acagcgaaga agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg
    22441 ttcctgacac aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg
    22501 aacaatcccc agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt
    22561 gtcgttaggt atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat
    22621 gagcaaatcg cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag
    22681 ttgtaacagc tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt
    22741 ggcgacatca cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat
    22801 tgatgaaccg agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata
    22861 ccgtgctcaa caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat
    22921 gctggataaa cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact
    22981 ggagagttta tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca
    23041 gcaaattttg cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt
    23101 actgcatggc gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca
    23161 atcatcccca gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca
    23221 ttctcaacac cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat
    23281 tgttacactg ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc
    23341 tctggtaaaa ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga
    23401 aatgtcctgg ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg
    23461 ggaagcagct gatgcatatc tcactgcctt taatttacgc ccaggggcaa acacccttta
    23521 ctggattgaa aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa
    23581 atttctcgct tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg
    23641 agtttttgta tgttcccatt ggtataatat
//
LOCUS       DAZPLI010000053        23514 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZPLI010000053 DAZPLI010000000
VERSION     DAZPLI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.053"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..2064
                     /locus_tag="ACHUU3_002016"
     CDS             <1..2064
                     /locus_tag="ACHUU3_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR2082087.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
     gene            2080..3099
                     /locus_tag="ACHUU3_002017"
     CDS             2080..3099
                     /locus_tag="ACHUU3_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFR2082088.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNANLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGNKATIN
                     SKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFMLESNGS
                     PVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATALI
                     NVKYD"
     gene            complement(3181..3519)
                     /gene="rcnB"
                     /locus_tag="ACHUU3_002018"
     CDS             complement(3181..3519)
                     /gene="rcnB"
                     /locus_tag="ACHUU3_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR2082089.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3738..4562)
                     /gene="rcnA"
                     /locus_tag="ACHUU3_002019"
     CDS             complement(3738..4562)
                     /gene="rcnA"
                     /locus_tag="ACHUU3_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFR2082090.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4683..4955
                     /gene="rcnR"
                     /locus_tag="ACHUU3_002020"
     CDS             4683..4955
                     /gene="rcnR"
                     /locus_tag="ACHUU3_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFR2082091.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      5047..5143
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5178..5966
                     /gene="thiM"
                     /locus_tag="ACHUU3_002021"
     CDS             5178..5966
                     /gene="thiM"
                     /locus_tag="ACHUU3_002021"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR2082092.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5963..6763
                     /gene="thiD"
                     /locus_tag="ACHUU3_002022"
     CDS             5963..6763
                     /gene="thiD"
                     /locus_tag="ACHUU3_002022"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR2082093.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6828..7646
                     /locus_tag="ACHUU3_002023"
     CDS             6828..7646
                     /locus_tag="ACHUU3_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFR2082094.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7698..8444
                     /locus_tag="ACHUU3_002024"
     CDS             7698..8444
                     /locus_tag="ACHUU3_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR2082095.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8418..9383)
                     /locus_tag="ACHUU3_002025"
     CDS             complement(8418..9383)
                     /locus_tag="ACHUU3_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR2082096.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9380..10384)
                     /locus_tag="ACHUU3_002026"
     CDS             complement(9380..10384)
                     /locus_tag="ACHUU3_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFR2082097.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10381..11658)
                     /locus_tag="ACHUU3_002027"
     CDS             complement(10381..11658)
                     /locus_tag="ACHUU3_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFR2082098.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11915..12967
                     /gene="fbaB"
                     /locus_tag="ACHUU3_002028"
     CDS             11915..12967
                     /gene="fbaB"
                     /locus_tag="ACHUU3_002028"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR2082099.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13286..14140
                     /gene="gatY"
                     /locus_tag="ACHUU3_002029"
     CDS             13286..14140
                     /gene="gatY"
                     /locus_tag="ACHUU3_002029"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR2082100.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14169..15426
                     /gene="gatZ"
                     /locus_tag="ACHUU3_002030"
                     /pseudo
     CDS             14169..15426
                     /gene="gatZ"
                     /locus_tag="ACHUU3_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15436..15888
                     /gene="gatA"
                     /locus_tag="ACHUU3_002031"
     CDS             15436..15888
                     /gene="gatA"
                     /locus_tag="ACHUU3_002031"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR2082101.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            15919..16203
                     /gene="gatB"
                     /locus_tag="ACHUU3_002032"
     CDS             15919..16203
                     /gene="gatB"
                     /locus_tag="ACHUU3_002032"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR2082102.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            16207..17562
                     /locus_tag="ACHUU3_002033"
     CDS             16207..17562
                     /locus_tag="ACHUU3_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR2082103.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            17610..18650
                     /gene="gatD"
                     /locus_tag="ACHUU3_002034"
     CDS             17610..18650
                     /gene="gatD"
                     /locus_tag="ACHUU3_002034"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR2082104.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            18756..19529
                     /locus_tag="ACHUU3_002035"
     CDS             18756..19529
                     /locus_tag="ACHUU3_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR2082105.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(19611..20510)
                     /gene="yegS"
                     /locus_tag="ACHUU3_002036"
     CDS             complement(19611..20510)
                     /gene="yegS"
                     /locus_tag="ACHUU3_002036"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR2082106.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            20916..21233
                     /gene="yegR"
                     /locus_tag="ACHUU3_002037"
     CDS             20916..21233
                     /gene="yegR"
                     /locus_tag="ACHUU3_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR2082107.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(21563..22924)
                     /gene="yegQ"
                     /locus_tag="ACHUU3_002038"
     CDS             complement(21563..22924)
                     /gene="yegQ"
                     /locus_tag="ACHUU3_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR2082108.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(23071..23403)
                     /locus_tag="ACHUU3_002039"
     CDS             complement(23071..23403)
                     /locus_tag="ACHUU3_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR2082109.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
BASE COUNT         6062 a         5510 c         6025 g         5917 t
ORIGIN      
        1 ggctatgttc caccggaaaa ctgggagcgg ggtattaatg cgttttatac ctcttattat
       61 gtgagtcagt attacagtga ctataaagcg tcgggtaata gtaagagtac atatgtacgt
      121 tttaacagcg ggttaaattt actggggtgg caactgcatt ctgatgccag tttcagtaaa
      181 acaaataaca atccaggggt gtggaaaagc aataccctgt atctggaacg tggatttgcc
      241 caacttctcg gcacgcttcg cgtgggtgat atgtacacat caagcgatat ttttgattct
      301 gttcgcttca gaggtgtgcg gttgtttcgt gatatgcaga tgttgcctaa ctcgaaacaa
      361 aattttacgc cacgggtgca ggggattgct cagagtaacg cgctggtaac tattgaacag
      421 aatggttttg tggtttatca gaaagaggtt cctcctggcc cgtttgcgat tacagatttg
      481 cagttggccg gtggtggagc agatcttgat gtcagcgtga aagaggcgga cggttcggta
      541 accacctatc tggtgcctta tgcagcggtg ccaaatatgc tgcaacccgg cgtgtcgaaa
      601 tatgattttg cggcgggtcg tagccatatt gaaggggcga gcaaacatag tgattttgtc
      661 caggcgggtt atcagtatgg ttttaataac ttattgacgt tgtatggtgg ctcgatggtt
      721 gcgaataatt attacgcgtt cactttgggg actggctgga atacacgcat tggtgccatt
      781 tccgtcgatg ccacgaagtc gcatagtaaa caagacaacg gcgatgtgtt tgacgggcaa
      841 agttatcaaa ttgcctacaa caaatttgtg agccaaacgt cgacgcgttt tggtctggcg
      901 gcctggcgtt attcgtcgcg tgattaccgg acatttaacg atcacgtttg ggcaaacaat
      961 aaagataatt atcgccgtga tgaaaacgat gtctatgaca ttgccgatta ttaccagaat
     1021 gattttggtc gcaaaaatag cttttccgcc aatatgagcc agtcattgcc agaaggttgg
     1081 gggtctgtgt cattaagtac gttatggcga gattactggg ggcgtagcgg cagtagtaag
     1141 gattatcagt tgagttattc caacaacctg cgacggataa gctataccct cgcggcaagc
     1201 caggcttatg acgagaatca tcatgaagag aaacgtttta atatttttat atcgattccc
     1261 tttgattggg gtgatgacgt ttcgacgcct cgtcggcaaa tatatatgtc taactcaacg
     1321 acgtttgatg atcaggggtt tgcctcaaat aatacgggat tatcaggaac agtagggagt
     1381 cgggatcagt tcaattatgg tgtcaacctg agtcatcaac atcagggaaa tgaaacgaca
     1441 gctggagcga atttaacctg gaacgcgccg gtggcaacag tgaatggcag ttatagtcag
     1501 tcgagtactt atcgacaggc tggagccagt gtttcagggg gcattgtcgc ctggtcgggt
     1561 ggcgttaatc tggcgaaccg tctttccgaa acgtttgctg tgatgaatgc gccaggaatt
     1621 aaagatgctt atgtcaatgg gcaaaaatat cgcacaacaa accgtaatgg agtggtggta
     1681 tacgacggaa tgacacctta tcgggaaaat cacctgatgc tggatgtgtc gcaaagcgat
     1741 agcgaagcag aattacgtgg caaccggaaa attgccgcac cttatcgcgg cgcggttgta
     1801 ctggttaatt ttgataccga tcagcgcaag ccctggttta taaaagcgtt aagagcggat
     1861 gggcaaccat taacgtttgg ttatgaagtc aatgatatcc acggtcataa tattggcgtt
     1921 gtcggccagg gaagccagtt atttattcgc accaatgaaa taccgccatc ggttaatgta
     1981 gcaattgata agcaacaagg actttcatgc acaatcacct tcggtaaaga gattgatgaa
     2041 agtagaaatt atatttgcca gtaatagtca ggtggttcta tggaaattcg cataatgcta
     2101 tttatattaa tgatgatggt tatgcctgtg agctatgcgg catgttatag tgagttatct
     2161 gttcagcaca atttggttgt tcagggggat tttgcactta ctcaaacaca aatagcgaca
     2221 tatgagcata attttaatga ttcgtcatgc gtgagtacaa atactatcac ccctatgagt
     2281 tcgtcggata ttattgttgg gctttataac gataccataa aattaaattt acattttgaa
     2341 tggaccaata aaaacaacat cacgttgtca aataatcaga ccagtttcac cagtggttat
     2401 tcagttacgg tgacacctgc ggccagtaat gcaaaagtga atgtttctgc ggggggcggc
     2461 ggttcagtga tgattaatgg tgttgcgaca ttatccagtg cttcatcatc gacacgcggg
     2521 agtgccgcag tacaatttct actgtgttta ttaggtggca agtcatggga tgcatgtgta
     2581 aatagctaca gaaatgcatt ggcacaaaat gctaatctga cattgtcata caacccgata
     2641 accacaacct gcaaaccgga cgatttatta attactttag acagtattcc cgtttcacaa
     2701 ttaccagcca caggtaacaa agcaacaata aatagtaaac aaggggatat tattctgcgt
     2761 tgtaaaaatt tattaggtca acaaaatcaa acatcacgga aaatgcaggt gtatttatca
     2821 agttctgatt tgttagccgg cagtaacacg atcctgaaag gtgcagaaaa taatggagtc
     2881 ggatttatgc ttgaaagcaa tggttctcca gtcacatttt taaatatcac tgataacagc
     2941 aaaggataca caaatttaaa agaaattgcg gcaaagtcaa aacttacaga tacaacggtt
     3001 tcaattccga taactgccag ttactacgtc tacgatacaa acaaagttaa atctggcgca
     3061 ctggaggcaa ccgcattaat caacgtgaaa tacgactaat aacaagcaag acgagcaagt
     3121 ggctaataat aaaaaataac aaggtgtctg catttccccc tcatgatgag ggggcttttt
     3181 ttagcgatga taaaaaatct caccgtcgta ggctttaatg attttaccgt cggtgtcgct
     3241 gatcaacacg tacgcgccac ccatataggt ccagtgcgtc ccggcatcag gcgcgggcag
     3301 gttacgtaac tgccactgct taatgttgta ctcatcggtg cggtacatct ctgggacggt
     3361 atcacctggt ttgaaatgct taaagtcagc aatgaaacta cttaactcat acttagaaat
     3421 tcccgaggta ttggtcgaac ccgcggtggc tggtgcggcc caggcggcac tggtaaccag
     3481 caaaagcaca cccagcaaca tcttattttt aatggtcata aactctccac tttggggatc
     3541 tgtaatatca gtcgcatatc tttcctgcca tacaatataa ctgacaagcg atattttgtt
     3601 agtgatccca tgatttcgta aaaagtgtaa atgaaaggat gccctaaaag catccttagc
     3661 gagcagagag tcagcggaaa tagctgattt aagaatcatc ctgggctgaa gataacatgc
     3721 aagcctaaag gattgagtta tcgcattatg cccatgaagc catgtacgcc catatacaca
     3781 ccgactaagc caatcaacag actggaaaaa tagggggcgc gtttagcgag agtgttaaat
     3841 ccgctccagc gttttgcgac ctgctgaacg ctgattgctg cgccaacgcc tacggtgaca
     3901 agcgttaacg ccaggccaat gctgaaactg acgaccagtg ttgcgcccag tgtcagggct
     3961 ttcaactgaa tgcatatcaa cagcacggta attgctgccg ggcaggggat aaggccaccg
     4021 gttaagccaa atagcaaaat ttgccagttg gtgacctctc taccatcaaa gcgtcgttta
     4081 atgtcattgg catgggctcg tgcatgggca tcctgatact cgccatgttc gtgatggtga
     4141 tgatgtccat ggtcgtggtg atcttcgtga tcgtgatgat gatgctcata atcatactcg
     4201 tgcatattct ccagccagtt gcgttcgccg cgccaggtac gccagaacat ccagaacgcg
     4261 gtgccaataa tgatcactgc ggaaatcagc tggagccacg gttctgctga ttgagcagta
     4321 aagcgcttgc tgatcaccat cccgccaaag gcaattaacc agaccactgc ggtatgcgaa
     4381 atagttgctg ccagtccgag catcaccgct tgtttaatgg tgcctttgat ggcgatgata
     4441 aacgccgcca tcatcgtttt tgagtgccct ggttccaggc catgaagcgc accaagtaag
     4501 atggcgctgg ggatgaagaa ccaggcgttt ccttgctgaa gaagagttgt aaattcggtc
     4561 atgataataa ttcttagtat taattcggca atctgattct actcccccca gtacctgata
     4621 ctacccccca gtagattaat agtgctatga tattttcata ttcttgttaa ccaggtgttg
     4681 ccatgtctca tacaatccgt gataaacaga aactgaaagc gcgtgccagt aagattcagg
     4741 gccaggtcgt ggcgctcaag aaaatgctcg acgagccgca tgaatgcgct gcagttttac
     4801 aacagattgc tgctatccgt ggcgcggtaa acggtctgat gcgggaagtg attaaaggtc
     4861 atctgacgga acacatcgtt caccaggggg atgagctaaa acgtgaagaa gatctggatg
     4921 tcgttctgaa ggtgctggat tcatatatca aataatttat taacgcgatt gtaaaactgc
     4981 cgtttttcct cgtttacaac gcgtgcgctg gacattacca tcctcctctg cgatttatca
     5041 tcgcaaccaa acgactcggg gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc
     5101 tgatctggat aatgccagcg tagggaagtc acggaccacc aggtcattgc ttcttcacgt
     5161 tatggcagga gcaaactatg caagtcgacc tgctgggttc agcgcaatct gcgcacgcgt
     5221 tacacctttt tcaccaacat tcccctcttg tgcactgcat gaccaatgat gtggtgcaaa
     5281 cctttaccgc taataccttg ctggcgctcg gtgcatcgcc agcgatggtt atcgaaaccg
     5341 aagaggccag tcagtttgcg gctatcgcca gtgccttgtt gattaacgtt ggcacactga
     5401 cgcagccacg cgctcaggcg atgcgtgctg ccgttgagca agcaaaaagc tctcaaacac
     5461 cctggacgct tgatccagta gcggttggtg cgctcgatta tcgccgccat ttttgtcatg
     5521 aacttttatc ttttaaaccg gcagcgatac gtggtaatgc ttcggaaatc atggcattag
     5581 ctggcattgc taatggtgga cggggagtgg ataccactga cgccgcagct aacgcgatac
     5641 ccgctgcaca aacactggca cgggaaactg gcgcaatcgt cgtggtcact ggcgaggtgg
     5701 attatgttac cgatggccat cgcgccgttg gcattcacgg tggcgatccg ttaatgacca
     5761 aagtggtagg aactggctgt gcattatcgg cggttgtcgc tgcctgctgt gcgttaccag
     5821 gcgatacgct ggaaaatgtc gcatctgcct gtcactggat gaaacaagcc ggagagcgcg
     5881 cagtcgccag aagcgagggg ctaggcagtt ttgttccaca tttccttgat gcgctctggc
     5941 aattgacgca ggaggtgcag gcatgaaacg aattaacgct ctgacgattg ccggtactga
     6001 tccgagtggt ggtgcgggga ttcaggccga tcttaaaacc ttctcggcac ttggcgctta
     6061 tggttgctca gttattactg cactggtggc gcaaaatacc cgtggcgtac agtcggtgta
     6121 tcgcattgag cctgattttg tcgccgccca gctcgattcg gtgttcagcg atgtgcgaat
     6181 cgataccact aaaatcggta tgttggcgga aaccgatatt gttgaagcgg tggcagaacg
     6241 gttgcaacgt tatcagatcc aaaacgtggt actcgacacc gttatgctgg caaaaagcgg
     6301 cgacccactg ctttcacctt cggcagttgc cacgctacgc agtcgattat tgccacaggt
     6361 ttcattaata acgccaaact tgcccgaagc agccgccttg ctcgacgcgc cacacgcgcg
     6421 caccgaacag gaaatgctgg aacaagggcg atcgctgttg gcgatgggct gtggcgcagt
     6481 gctaatgaaa ggtggtcatc tggatgatga gcaaagcccg gactggctgt ttacccgcga
     6541 gggtgaacaa cggtttaccg caccgcgcat tatgaccaaa aacacccacg gtactggttg
     6601 tacactctct gcggcgttgg ctgcactacg cccgcgccat acaaactggg ctgacaccgt
     6661 acaggaggca aaaagctggc tttcatcggc gttagcccag gccgacacgc tggaagttgg
     6721 tcacggtatt ggtccggttc accacttcca cgcctggtgg tgagcaccgt actatagctg
     6781 ctttggttat tgtcagcctt tggcactgac attcagggat gaaaccaatg cagctaagaa
     6841 ttaccagtcg aaaaaaactc accgctctat tatgcgctct tgggctaatt tccatcgttg
     6901 caatttatcc gcgtcagacg gtgaattttt tctattcgac agcagttcag attacagact
     6961 acatccactt ctacggttat cgcccggtta aatctttcgc tattcgtatt cctgccagtt
     7021 acaccattca cggaatagat gtttcgcgct ggcaggagcg gatcgactgg cagcgtgtgg
     7081 caaaaatgcg cgataacggt attcgcttac aatttgcctt tattaaggcg acggaaggcg
     7141 aaaagctggt ggacccctat ttttcgcgta actggcaact aagccgcgaa aatggcttgc
     7201 tgcgcggggc atatcattat ttctccccgt cggtagcagc accggttcag gtgcgattat
     7261 ttctgcaaac ggtagatttc tcacaaggcg atttccctgc ggtactggac gtagaagagc
     7321 gaggaaaatt atcggcaaaa gaattacgca agagggtaag tcagtggcta aaaatggtcg
     7381 aaaaaagtac ggggaaaaag ccgattattt actcaggagc cgttttttat cacacgaatc
     7441 tggcgggcta tttcaatgaa tatccgtggt gggtggctca ctactatcaa cgtcgtccgg
     7501 acaatgacgg catggcctgg cgcttctggc agcattccga ccgtggacag gtagatggca
     7561 tcaatggtcc ggtggatttt aatgtgttta atggcacggt ggaggagttg cagggattcg
     7621 ttgatgggat taaagaaacg ccttaaataa gaagcagtga tatactccca ccagactccc
     7681 tggcaggaaa aagacaaatg gaacaagcgc atacccagct gattgcccaa ctgaacgaac
     7741 gtattttagc ggcggataac acgccgcttt atattaagtt tgccgaaacg gtaaaaaatg
     7801 ccgtgcgcag cggggtgctg gagcatggca atattttgcc cggtgagcgt gatttaagtc
     7861 agttaaccgg cgtttcgcgc attacggtgc gcaaggcgat gcaggcgctg gaagaagagg
     7921 gcgtggtgac gcgttcgcgc ggttacggca cgcagatcaa caacatcttc gaatactcgt
     7981 tgaaagaagc gcgaggtttt tctcagcagg tggtgttgcg cgggaaaaag cccgatacgc
     8041 tatgggttaa caagcgtgtc gtgaaatgtc ccgaagaagt cgcgcagcag ctggcggtcg
     8101 aagcaggaag tgatgtcttt ttgcttaagc gtattcgcta tgtcgatgaa gaagcggtat
     8161 cgattgagga atcgtgggtt ccggcgcatt taattcatga tgttgatgcc atcgggattt
     8221 cactttatga ctatttccgc agccagcaca tttacccaca acgtacgcgt tcccgcgtta
     8281 gcgcccggat gccggatgcc gagtttcagt cacatattca gttagatagc aaaataccgg
     8341 tgctggtgat caagcaagtg gcgcttgacc aacagcaacg gcctattgag tacagcatta
     8401 gccactgtcg cagcgatcta tacgtttttg tgtgcgagga gtagttcctc gcgcgttggc
     8461 gcacaatcac cgccccgatg cccgacaacc cacgacgcca ctgcattgcc cagtaatacg
     8521 gcatccgcca gtggcagacc agaggccagc ccggcaagta cgccacctgc atgactgtcg
     8581 cccgccccaa tggtgtctac aacttgcgtt ggaaatgccg gaatgcagcc agaagcgtcg
     8641 ttgctgaaat accatgcgcc ttctttatcg agacgaacga tcaacggcgc ggcaaatttc
     8701 tcctgccatt gctcgccaag tgttgttatc tctgcggata aagcaaaacg ttcggcggca
     8761 atctcagcct cttgacgatt gagcgacact aaaggtcgac aggccatgat ccgcgccagt
     8821 aatgcatccg ggatatcgcc aatacgtggg ccaaaatcga taaacggcgt cacgtcttgc
     8881 agctcttcca gccattccac taacaattcg ccgcagggcg aggccagttg ataaccggaa
     8941 aaatagagca ggctgccagg cgcaacggtt aatcgcgcca gccactggcg attccactga
     9001 ttttccacac cgctgaatga cataaaagtg cgttcgccat ccggctcaac cagcgccaga
     9061 caccagccgt tatcaccttc ggcgttatcg atcaggctga ttaagccctc ttttgccatc
     9121 cggttgcgaa taatctccgc ccacacgccc tgaccgagcg gcaaggcatt acccgcttcg
     9181 atgccgaggc gctttaacgc cacggcaata ttcagtgcgc agccgccaac gttaacgctc
     9241 tgctgtttca gttcgatatc gcacccacgc caggggaggg cataagcgtc ggcgatcacg
     9301 tcaatgaccg ccgcgccgac gaccatcacc ggctgacgcg tggttaattc aggcagcagc
     9361 gtgtgtaatc gagcgccgct cataccgcct cccgttgttg acgatatttc gccagcgctg
     9421 tggcatagcg gttgaaatca agctgattta ccgcatccag ttccgccttt aacgcaggat
     9481 cgatagcgtt aacgccatgc aacgcgccgc aaattgccgt cgccatagca ccgatggtgt
     9541 ctgtgtcgcc gccaaggtta gcgcacagga cggcgcagcg attcgggtcg gtttgtgcta
     9601 gttcaaccag cgcaatggcg cacggaacgg actcaatagt gctggtacct gcgccaacga
     9661 cctggtaaag ctgttcgctg gcggattcgg tgccgtcggc attgcgcaca attttcagcg
     9721 caatctccag ccgcgccgcc agtgaggcgc tgaacgtagt gatgcgtttt tgctgtgcat
     9781 gtcgcgcaat tgaaggcagg gaatcaacaa tcgctgacca gctttctccg tcaatggcac
     9841 gagaaatcgc ccatgcgatg actaccgcgc ctgcaaccgc cagatcggat ttatgggtcg
     9901 ggctggacgc cagcgctaca tcatcaataa aggaatcaac atcacgcgcc ggaagcaaac
     9961 aacctaatgg cgagacgcgc atcgctgcgc cgttggtcac gccgttgttt tccagttcag
    10021 caacgggttt accgtcgcga atggcgttaa gcgcaatctt cgaggtcgga cctagtacgt
    10081 ttttgttaaa ggcgtcgaaa cgcagcgccc agtcgagaat attacgccca atcagatccg
    10141 gatcgatctt gccttcacgt tccagtaacg catccgccag acacagcgcc atcgaggtat
    10201 cgtcggtgaa ttcggcgcgg ttaaaataac aggccgcgtt attctccttt ggtccaggaa
    10261 gaaaacggtc aatccagcca aagtgtgctt taacgcggct gcgtggccaa agctcggagg
    10321 gcatccccat cgcatcccct aacgcctgcc cataaagagc accgagaata cgttctgttt
    10381 tcatttaact tccccttgtg tcaacgcaat atcgcgatca tcgaccttga tagccgtaat
    10441 ttcgttgtcg gattcgcgga aaaaaatcat gaacagcacg gcgataatgg caatcatcac
    10501 cgcgccgaaa gtccacatcc cggaccagtt gaaagtcagt ccgtttaccg gttcctgata
    10561 agcgaacatc ttttccatca tcacaccgcc aagacgatag ccgagcaaac tgccgaagcc
    10621 ctggcagcag agcgtgatca gcccctgcgc agcggtacgc atatgcacgg gggctttttt
    10681 atcgacatag atgtaagcgg taacgtagta aaaatcgtaa cttacgccgt gaagcaaaat
    10741 accgaggaac agtaacgcgt aggtgaaata ttcatccgta ctaccgtaaa taaagaagcc
    10801 atagcggatc gcagcggtga ccagaccaag caataatacc tttttgatac caaagcgttt
    10861 agtgaaaaac ggcaatgcca gcataaagaa gatttcagag aactggccga gcgtcatcca
    10921 gccggtggcg tttttcatgc caacttcggt cagataaccg ttggcaaaga tgtaatagaa
    10981 cgccagtggc atcgcaaaca ggaatgaaca gaagaaaaag acgaggaagt ttttatcgcg
    11041 cagcaggatc agcgcatcca ggccgagcat gactttaata tccattttgc cggtgctttt
    11101 tggtggcgtg tcgggcagga aaaacgcaaa cacaccgagc agagcagaac ttccggcggt
    11161 aatcagcagc gggatgttag tcggtgagat atcggcatac cccagtattt gcggcaagaa
    11221 accacatgcc agaccggagg caatccagcc gatagtgccc atcacacgaa tgcgcgggaa
    11281 atcacgctcc acatccggca cgttggcaaa agcgatgctg ttagtcagcg caatggtcgg
    11341 catataggtt agagagtaag ccagcagtaa cggaaagaac ccggcaaaag tggtctgttg
    11401 cgcagcgaaa tacatcagca gcgcgcctgc gaacatcaat accgccagca ctttttgcgc
    11461 cgagaaaaag cggtcagtga tggagccaac cagaattggc gacaggatcg ccgcaatggc
    11521 ggtacaggca tacgaccagc caatttctcc ggcactaaaa ccgcttttac ttaaccacaa
    11581 ccacaatggc acaaaccacg cgccccagat aaaccattca acaaacatca tgaacgacag
    11641 ctttgctgtt gttttcatct tttaattcct tcatgacagg taatgtaagg tacgcgatga
    11701 caataccact taacaatacc ttttaaatac ctttgcggtg aaaacttgac cgcgctaaca
    11761 ttttttctga tgaatcgagc caacagaaaa cgctgaaaaa acatccaaaa gatggaaaaa
    11821 ctcgcccgta acgaccagga ttatgattgc ccgcaaaagg cgggccagga caatccatag
    11881 ccgatatcca atcggaattt acgggagcat agtaatgaca gatattgcgc agttgcttgg
    11941 caaagacgcc gacaaccttt tacagcaccg ttgtatgaca attccttctg accagcttta
    12001 tctccccgga catgactacg tagaccgcgt aatgattgac aataatcgcc cgccagcggt
    12061 gttacgtaat atgcagacgt tgtacaacac cgggcgtctg gctggcacag gatatctttc
    12121 tattctgccg gttgaccagg gcgttgagca ctctgccgga gcttcatttg ctgctaaccc
    12181 gctctacttt gacccgaaaa acattgttga actggcgatc gaagcgggct gtaactgtgt
    12241 ggcgtcaact tacggcgtgt tggcgtcggt atcgcggcgc tatgcgcatc gcattccatt
    12301 tctcgtcaaa cttaatcaca acgagacgct aagttacccg aatacctacg atcaaacgct
    12361 gtatgccagc gtggagcagg ccttcaacat gggcgcggtg gcggttggta cgactatcta
    12421 ttttggctcg gaagagtcac gtcgccagat tgaagaaatt tctgcggcct ttgaacgcgc
    12481 gcacgagctg ggcatggtga ccgtgctgtg ggcctatttg cgaaactccg cctttaagaa
    12541 agatggcgtt gattaccatg tttccgccga cctgaccggt caggcaaacc atctggcggc
    12601 aaccatcggt gcagatatcg tcaaacaaaa aatggcggaa aataacggcg gctataaagc
    12661 aattaattac ggttacaccg acgatcgtgt ttatagcaaa ttgaccagcg aaaacccgat
    12721 tgatctggtg cgttatcagt tagctaactg ctatatgggc cgggctgggt tgataaactc
    12781 cggcggtgct gcgggcggtg aaactgacct cagtgatgca gtgcgtactg cggttatcaa
    12841 caaacgcgct ggcggaatgg ggctgattct tggacgtaaa gcgttcaaga aatcgatggc
    12901 tgacggcgtg aaactgatta acgccgtgca ggacgtttat ctcgatagca aaattactat
    12961 tgcctgatac gcctcatacg aacagcacca ccccgtagga cggataaggc gtttacactg
    13021 catccggcaa cctatgcctg atgcgacgcg gaagcgtctt atcatgccta catagcactg
    13081 ccacgtatgt cggaaaaggc gtttacaccg catccggcat aaaaacacgc gcactttgct
    13141 acggcttccc tatcgggagg ccgttttttt gcctttcact cctcgaacag ttttcatatt
    13201 gtcgttttgt gatcgttatc tcgacattta aaaacaaatc atttcattat attttgaaat
    13261 cgaaaacaag cgacaggata tgaaaatgta cgtggtatcg acaaagcaga tgctgaacaa
    13321 cgcacagcgc ggcggttatg cggttccggc attcaatatt cacaatctcg aaacgatgca
    13381 agtggtggtg gaaaccgctg ccaacctgca tgcgccggtc atcatcgccg gaacgcctgg
    13441 cacattcact tatgctggta cagaaaatct gttggcgctg gtcagcgcga tggcgaagca
    13501 atatcaccat ccactggcaa ttcatctcga ccatcacacg aaatttgacg atatcgctca
    13561 gaaggttcgt tctggcgtgc gctcagtcat gattgacgcc tcgcatttgc cttttgcgca
    13621 aaatatttca cgggtcaaag aggtggtgga tttttgccat cgctttgatg tcagcgtcga
    13681 agcggagctg gggcaacttg gcggccagga agatgatgtg caagtcaatg aagccgatgt
    13741 tttttatacc aaccccgctc aggcgcgtga atttgccgag gcaaccggaa ttgattccct
    13801 ggcggtcgcc atcggcacgg ctcatgggat gtatgccagc gcaccggcgc ttgatttttc
    13861 tcgactggag aacattcgcc agtgggtgaa cttaccgctg gtgctgcatg gcgcgtcagg
    13921 gttatcgact aaggatattc agcaaaccat caaactgggg atatgcaaaa tcaacgttgc
    13981 aacggagctg aaaaatgcct tctcgcaggc gttaaaaaat tacctgaccg agcaccctga
    14041 agcgaccgat ccccgggatt atttgcagtc ggctaaatcc gcaatgcgcg atgtggtgag
    14101 caaagtgatt gccgattgtg gctgcgaggg cagggcataa cgcacctgcc atttaacaag
    14161 gaaaaaacat gaaaacgtta attgcccggc ataaagctgg tgaacatatc ggcatatgtt
    14221 cagtctgttc tgcccatccg ttggttatcg aagcggcgct ggcatttgat cgcaacagca
    14281 cgcgcaaagt gctgattgaa gcaacgtcaa accaggtcaa tcaatttggc ggttataccg
    14341 gaatgacacc ggcagacttt cgcgaatttg tttttacgat tgccgataaa gtcggatttg
    14401 cacgcgaacg cattattctc ggcggcgatc atctggggcc aaactgctgg cagcaagaaa
    14461 atgcggatgc ggcgatggaa aaatccgtcg agctggtaaa agcatatgtt cgtgctggct
    14521 tcagtaaaat tcatcttgat gcgtcaatgt cctgcgcggg ggatcccata ccgttagcac
    14581 cagaaacggt tgcggaacga gctgctttgc tttgctgcgg aaagtgtggc gacagattgc
    14641 cagcgtgagc aactgagcta tgtcattggc actgaagttc cggttccggg cggtgaggcc
    14701 agcgccattc agtcagtaca catcacccat gttgaagatg ccgccaatac tttacgtacg
    14761 catcaaaagg cctttattgc ccgtgggctg acagaggcgt taacacgcgt gattgccatc
    14821 gtggtgcagc cgggcgtgga atttgatcac agcaatatta tccattatca gccgcaggaa
    14881 gcgcagccgc tggcgcaatg gatagaaaac acccgaatgg tttatgaagc acattctacc
    14941 gattaccaga cccggacggc ttattgggaa ttagtccgcg atcactttgc aatattgaaa
    15001 gtcggtcccg cattaacctt tgctttacgc gaggcgatat ttgcactggc acaaattgag
    15061 caggaactta tcgcccctga aaatcgcagc ggttgcctgg cggtaattga agaagtgatg
    15121 ctcgacgaac cgcaatactg gaaaaaatat tatcgtacgg gttttaacga ttcattactg
    15181 gatattcgtt acagcctgtc ggatcgtatt cgttattact ggccgcatag tcggattaaa
    15241 aatagcgtcg aaacgatgat ggtgaatctt gaaggcgtgg acatcccact gggcatgatt
    15301 agtcagtatc ttcccaaaca atttgaacgc attcagtccg gggaattatc agcaataccg
    15361 catcagctga ttatggataa aatttatgat gttttgcgcg cctatcgcta cggctgtgcg
    15421 gaataaggac ggtatatgac taacctgttt gttcgtagcg gaatttcttt tgtcgatcgt
    15481 agcgaagttt taacccatat cggtaatgag atgctcgcca aaggtgtggt tcatgatacc
    15541 tggccacagg cattaattgc cagagaagca gaattcccta ccgggataat gcttgagcag
    15601 cacgccattg caataccgca ttgtgaggcg attcatgcta agtcgtcagc catttatctg
    15661 ttaaggccaa caaataaagt tcattttcag caagcggatg atgataacga cgtggaggta
    15721 tcgttggtta ttgcgttgat tgtggagaat ccgcagcagc aattgaaact tttacgctgt
    15781 ttatttggca agttacaaca gcccgatatc gtcgagacac taatcactct tcctgaaacc
    15841 cagttaaagg aatacttcac aaagtatgtt ttagattcag acgaataaat ccctctgtaa
    15901 caataaaaag gactatttat gaaacgcaag attattgtcg cttgcggagg cgcggttgcg
    15961 acctctacga tggcggcgga agaaattaaa gagttgtgtc agaatcatag tattcctgtt
    16021 gaattaatcc agtgtcgggt taatgaaata gaaacctata tggatggtgt gcatttgata
    16081 tgcaccactg ccaaagtgga tcgtagtttt ggcgatattc cgttagttca cggcatgcct
    16141 tttatttctg gtgtcggtat cgaagcatta caaaataaaa ttctgactat cttacagggg
    16201 tgacctatgt tttcagaagt catgcgttat attctcgacc tcggccctac ggtgatgctt
    16261 ccgattgtca tcattatttt ttctaaaata ttaggcatga aggcaggcga ttgctttaaa
    16321 gcgggtctgc atatcgggat tggctttgtt ggcattggcc ttgtgattgg cttaatgctg
    16381 gattccattg gtccggcggc gaaagcgatg gcggaaaatt tcgacctgaa tctgcatgtg
    16441 gtcgatgtcg gctggccggg ctcttcacca atgacctggg cgtcgcaaat tgcgctggtg
    16501 gcgattccga ttgcgattct ggttaacgtg gcgatgttac tgacccgtat gacgcgggtg
    16561 gtaaatgttg atatctggaa tatctggcat atgaccttca ccggcgcgtt gctgcatctg
    16621 gcaaccggtt catggatgat agggatggca ggtgtggtaa ttcacgcggc gtttgtttat
    16681 aagctcggcg actggtttgc ccgcgatacc cgaaatttct ttgagctgga aggtattgct
    16741 attccgcacg gtacgtcggc gtatatgggg ccgattgcgg tgctggtcga tgctatcatc
    16801 gagaaaatcc caggcgttaa ccgaattaaa tttagcgccg acgatattca gcgcaaattt
    16861 ggtccatttg gcgagcctgt caccgtgggt tttgtgatgg ggctgattat cggcatcctc
    16921 gcgggttacg atgtcaaagg tgtattgcag ctggcggtaa aaacggcggc agttatgctg
    16981 ctgatgccac gggtgattaa acccatcatg gatggtttaa cgcccatcgc taagcaggcc
    17041 cgtagtcgtt tacaggcgaa gttcggcggt caggagttcc tgattggcct ggatcccgca
    17101 ttactgctgg ggcatacggc ggtggtatcg gcaagcctga tttttatccc gctcaccatt
    17161 ttaattgctg tttgtgttcc gggtaatcag gtactgccgt ttggcgatct tgccaccatc
    17221 ggcttcttcg tggcgatggc ggtcgcggtg catcgtggaa atctgttccg caccttaatc
    17281 tcgggtgtca tcattatgag catcaccctg tggatcgcga cgcaaactat tggtttgcac
    17341 acccaactgg cggctaatgc tggggcgtta aaagccgggg gtatggtggc ttcaatggat
    17401 cagggcggtt ctcccattac ctggttactg attcaggttt tctccacgca aaatattccc
    17461 ggtttcatta ttatcggcgc aatttatctt accggtattt tcatgacctg gcgtagagcg
    17521 cgtggtttta ttaaacaaga gaaaaccgtt ctcgcagaat aatttttacc tgagggggag
    17581 gttatccccc ttaacaatca ggagttttta tgaaatcaat ggtgaatgat actgatggta
    17641 tcgtgcgcgt tgcagaaagc gtcattcctg aaattaaaca tcaggatgag gtgcgggtaa
    17701 aaattgccag ctcgggctta tgtggttccg atttacccag gatatttaaa aatggtgcac
    17761 attattatcc aataacgtta ggccatgaat ttagcggcta tattgatgcg gtgggatccg
    17821 gtgttgatga tttacaccct ggcgatgcgg ttgcctgtgt gccgttatta ccctgtttta
    17881 cttatccaga gtgtttgaaa gggttttatt cccagtgcgc aaaatatgat tttattggct
    17941 cgcggcgtga tggtggattt gctgaatata ttgtcgttaa gcgaaaaaat gtctttgctc
    18001 tacccacgga tatgcctatt gaggatgggg cttttattga gccgattacc gttggcctgc
    18061 atgcttttca tttagcgcaa ggttgtgaga ataaaaacgt tattattatt ggtgccggaa
    18121 ccattggcct gctggctatt cagtgcgctg tcgcgctggg agcaaagagt gtgacggcga
    18181 tcgacattag ctcagaaaaa ctggcattgg caaaatcttt cggtgcgatg caaacattta
    18241 acagtagcga aatgagcgcg ccgcaaatgc agagcgtttt acgcgaactg cgctttaatc
    18301 agcttatcct cgagacggct ggcgtaccgc aaactgtcga actggcggta gagattgccg
    18361 gtcctcatgc ccaactggcg ctggtgggca cgttgcatca ggatctgcat ttaacatcgg
    18421 caacgtttgg caaaatattg cgtaaagagc tgacggttat cggcagttgg atgaactatt
    18481 ccagcccttg gccggggcag gagtgggaaa cggcgagccg gttgctgaca gaacgtaagt
    18541 taagcctgga gccattaatc gctcaccgtg gaagctttga aagcttcacc caggcggtgc
    18601 gtgacatcgc tcgtaatgcc atgccgggca aagtgttgct cattccatga aaccgcgggc
    18661 cagcgtgatg ctggcccggt attgtgcaaa acagatcatt caccaatggt cccccttcgt
    18721 ttacactagc cacaattgaa tatgggtaaa tgacgatgaa ttcattcgag cgaaggaata
    18781 agatcatcca attagtgaat gaacagggaa ccgtgcttgt tcaggatctg gcgggagtat
    18841 ttgctgcctc ggaagcgaca atccgtgccg atttgcgctt tctcgaacaa aaaggcgtgg
    18901 ttacgcgctt tcatggcggt gcggcgaaaa taatgtccgg taatagtgaa accgagaccc
    18961 aggaagtcgg gtttaaagag cgatttcagc tcgccagcgc gccaaaaaac agaatagcgc
    19021 aggcggcagt caaaatgatc catgaaggga tgaccgttat tctcgacagc ggaagtacga
    19081 caatgcttat cgccgaagga ttaatgaccg ctaaaaatat cacggtgatt accaacagtc
    19141 tcccggcagc gtttgccctt tccgaaaata aagacattac tctggtcgtc tgtggtggca
    19201 cggtccgcca taaaacgcgc tcgatgcatg gttccattgc cgagcgttca ttgcaggata
    19261 ttaatgccga tttaatgttt gtcggtgctg atggtattga tgcggttaat ggtattacga
    19321 cctttaatga aggttattcg attagcggtg cgatggtcac cgccgctaat aaagtgattg
    19381 ccgttctcga ttcatcgaaa tttaaccgtc gcggttttaa tcaggtactg ccaatcgaaa
    19441 aaattgacat tattattacc gatgatgctg tatcagaggt ggataaactg gcattgcaga
    19501 agacacgggt aaaattaatt acggtgtagt attgttctgt atgccggata aggtatttac
    19561 accgcatccg gcatttcgga ttctccctgg ttcaagagag aatagtttga ttaacgcaat
    19621 aatagacaat ctggtggtaa tcgacaacgc aacgccgccg gaagtatttc aatatgaaag
    19681 ttttgtccgc tcaacggttc gccatcaaga ttaaaagtga tttcgtgtgg ggcttgtata
    19741 tcaaaccacg acgaagcgcc ttcgataata ttcgggttat cttcgtcaga ttttaaggtt
    19801 gatacgagag ccggaagaat ttcatcgccg gtaaaaatgc gcagttgcag caagccatcg
    19861 ttaattaacg cgttcggaca caattgctga ccgccaccgg cctgacgccc gttaccaata
    19921 ccaatgacca gggcgtcacc ttgccagtga aagttttcac cgcggatttc acaacggtcc
    19981 ggttgcagag tatccatgcg cattaagcca tgaatgatgt aagagacgcc acccagcgcg
    20041 gcttttaatt tttccggcgt ttctgtggta atacgcgtcc caaatccgcc tgtcgccata
    20101 ttaataaaac aggtttgttt gttgacctgc gccatatcta tcgcaatggc gttaccggca
    20161 attgccagtt tcagcgcctt atccagtgcc tcaggaatcc ctacactggt ggcaaaatca
    20221 ttggcggttc ctaatggcaa aattcccagc gcgggtatgc catccccctc acactgaatc
    20281 aacgccgtag aaacttcatt aatggtgcca tcgccaccac cggcaatcac cgttgcgacg
    20341 cccaacttcc gggcctcctc tacatatcgt gcggcatcgc ctttctccca ggtgacccgc
    20401 acatggatcg tcattccttc ctcacgcaac agcataattg cttcgcgcaa gggtagattg
    20461 tcagtacttt tgccattaag aatcagtaag ctggcgggaa attctgccat gatcgtatgt
    20521 gcctttatga ttggtctgta gatagtgtag agcagaaaac aaaaggtgga gtcagaacag
    20581 gatgaaagtc ggaggataaa aagtcagttc aagtatggag gattcagtgg ttaccaccag
    20641 gagcttgcaa tggggacagc gaatactaac ggctgccacc gataaatttc aaaaaagagc
    20701 atatacctaa tattcaacta aacagtggca tcttcaatat aatatattaa agcccccatg
    20761 gagttaccct gaagggcctc aatgtccgta attcctactt atgtaggaaa tgttgtacag
    20821 aacatttatt ataatcctat tcaattataa taatcatgcc attattatat ttaaacacta
    20881 gagagtgtcg ttggtattta atgggggaag gtgagatgaa aaagatagct gctatatcat
    20941 taattagtat ttttcttata tctggttgtg ctgtgcataa tgatgaaaca agtatcggta
    21001 aatttggtct tgcatataaa agtaatattc agcgtaaact cgataaccaa tactacaccg
    21061 aagccgaagc ttctttagct aggggcagaa tatctggtgc agaaaatata gtaaaaaatg
    21121 atgcagccca tttctgtgtt actcagggca aaaaaatgca gatagttgac ctgaagacag
    21181 aaggtgcagg attacatggc gtcgctcgtc tgacattcaa atgtggagag tgagaatatt
    21241 ttttggtaag catcaaacat gcgcgttctg gttgtgctta gccggaacct gtgcgagcac
    21301 gatgccgtta cgtgaaaggc atcgtgctat gaagggagat tctatcgatg tggtcaatgg
    21361 aagacggtga ccagggatag ggcttatgca taaaaaataa gcccgtgtaa gggagattac
    21421 acaggctaag gaggtggttc ctggtacagc tagcatttta tgggttatgt ttttcagcga
    21481 aacggatgat aaccttaata aatgcagctg tatgtgatcg gtttctaaga attttccatc
    21541 cgggaaaaat aatcgaaatt aatcacttac cgtgtgggtt acgcgtggtt tccccggaga
    21601 aattacgcat cagcagcgcg taatttagct caagatcctg cgggaccggg agccacacag
    21661 tataaccatc gcctggtgcg accggcatcg cttcaccttt ggcgttttcc atgtgctcaa
    21721 gggtaaagtt aatgttgcct tgcggcgtca tcagctcaag gctgtcgcca acggagaatt
    21781 tatttttcac cgctaccgct gcgaggtccc ccttgcgctc accggtaaac tcaccaacaa
    21841 actgctggcg gtcagaaact gaataaccgt attcgtagtt ctgataatcg tcgtgagtat
    21901 gacgacgcag gaaaccttcg gtatagccac gatgcgccag accttccaga gtttccagca
    21961 ggctggtatc gaacggtttg cccgcagcgg catcatcgat agctttacga taaacctgtg
    22021 cggtgcgtgc gcaatagtag aaagatttag tacggccttc gattttcagc gaatgcacgc
    22081 ccattttggt caggcgttct acatgggcga tggcgcgcag atctttcgag ttcatgatgt
    22141 aagtgccgtg ctcatcttca aacgcggtca tatactcgcc cggacgctgg gcttcttcga
    22201 tcataaacac tttgtcggtt ggcgcgccga tacctagcgt cggctcaaca ttttgcaccg
    22261 gaatcggctc gtacttgtgt acgatgttgc caacgtcatc ttctttccct tcctggacgt
    22321 tgtactccca gcggcaggcg ttggtgcagg taccctggtt cggatcgcgc ttgttgatat
    22381 agccagagag caggcagcga ccggagtagg ccatgcacag cgcaccgtga acgaagatct
    22441 caatctccat atccggcacc tgattgcgga tctcttcaat ctcttccagc gacagctcgc
    22501 gagagaggat cacgcgggtc aggcccattt gctgccagaa tttcaccgtc gcccagttta
    22561 cggcgttagc ctgcaccgag aggtggatcg gcatttcagg gaagtgctca cgcaccagca
    22621 taatcagccc tggatcggac ataatcagcg catccggccc catttccacc accggtttca
    22681 ggtcacggat aaaggttttc agcttggcgt tgtgcggtgc aatgttgacc acgacataaa
    22741 actttttccc cagcgcgtgg gcttcattga tgccgagctg aagattttcg tggttgaatt
    22801 cgttgttgcg cacacgcagg gagtaacgcg gctggcccgc ataaacagca tctgcgccat
    22861 aagcgaaagc gtaacgcata tttttcagcg ttcccgccgg ggaaaggagt tccggtttaa
    22921 acataattct ctcgttctga tgacaggtca gctccgcttc acctgatgaa gcggttaggg
    22981 gagtgccccc actttaaggg cgggcattgt agcgctgtgg ggggatctgg taaagcgccg
    23041 gacgccgaca acccacgccc ggcatcgtaa ttacgtattg tctttcaccg tctggcttgt
    23101 gccattggct ttaacggagg caattccggt ttcacgagat tgcgcggtgg cgtacatctg
    23161 gctggagccg ataatttgat gattggcggc tttcagattg aaatagaatt tgccgttact
    23221 tgccgttttt ttctcatagc gttcttcttg cgggctgttg ctacgcaccg acgcgatgcc
    23281 cttttccgca gaggcttttg atgtatacag ctcgctggtg aggatagtct caccattgcc
    23341 cgcttttagc acaaaccgga actgattatc actgctctta ctgagttcaa accaaccagc
    23401 cataatgact ccttgattga aattgcgtat cgaaaacagc atctaaaggt gctgtttacc
    23461 gattaaagta tgagcgattt tctgatgcct gccagggcgg agagataatg tcag
//
LOCUS       DAZPLI010000054        22971 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZPLI010000054 DAZPLI010000000
VERSION     DAZPLI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22971
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.054"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..674)
                     /gene="focB"
                     /locus_tag="ACHUU3_002040"
     CDS             complement(<1..674)
                     /gene="focB"
                     /locus_tag="ACHUU3_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFR2082110.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(696..2218)
                     /locus_tag="ACHUU3_002041"
                     /pseudo
     CDS             complement(696..2218)
                     /locus_tag="ACHUU3_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
     gene            complement(2248..2661)
                     /gene="hyfJ"
                     /locus_tag="ACHUU3_002042"
     CDS             complement(2248..2661)
                     /gene="hyfJ"
                     /locus_tag="ACHUU3_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR2082111.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(2654..3412)
                     /gene="hyfI"
                     /locus_tag="ACHUU3_002043"
     CDS             complement(2654..3412)
                     /gene="hyfI"
                     /locus_tag="ACHUU3_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR2082112.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3409..3948)
                     /gene="hyfH"
                     /locus_tag="ACHUU3_002044"
     CDS             complement(3409..3948)
                     /gene="hyfH"
                     /locus_tag="ACHUU3_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR2082113.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(3958..5673)
                     /gene="hyfG"
                     /locus_tag="ACHUU3_002045"
     CDS             complement(3958..5673)
                     /gene="hyfG"
                     /locus_tag="ACHUU3_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFR2082114.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(5663..7243)
                     /gene="hyfF"
                     /locus_tag="ACHUU3_002046"
     CDS             complement(5663..7243)
                     /gene="hyfF"
                     /locus_tag="ACHUU3_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR2082115.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7248..7898)
                     /gene="hyfE"
                     /locus_tag="ACHUU3_002047"
     CDS             complement(7248..7898)
                     /gene="hyfE"
                     /locus_tag="ACHUU3_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR2082116.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIMMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(7910..9348)
                     /gene="hyfD"
                     /locus_tag="ACHUU3_002048"
                     /pseudo
     CDS             complement(7910..9348)
                     /gene="hyfD"
                     /locus_tag="ACHUU3_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(9365..10312)
                     /gene="hyfC"
                     /locus_tag="ACHUU3_002049"
     CDS             complement(9365..10312)
                     /gene="hyfC"
                     /locus_tag="ACHUU3_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR2082117.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10323..12341)
                     /gene="hyfB"
                     /locus_tag="ACHUU3_002050"
     CDS             complement(10323..12341)
                     /gene="hyfB"
                     /locus_tag="ACHUU3_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFR2082118.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFITSMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVVCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12341..12958)
                     /gene="hyfA"
                     /locus_tag="ACHUU3_002051"
     CDS             complement(12341..12958)
                     /gene="hyfA"
                     /locus_tag="ACHUU3_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR2082119.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13211..13681)
                     /gene="bcp"
                     /locus_tag="ACHUU3_002052"
     CDS             complement(13211..13681)
                     /gene="bcp"
                     /locus_tag="ACHUU3_002052"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR2082120.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(13681..14253)
                     /locus_tag="ACHUU3_002053"
     CDS             complement(13681..14253)
                     /locus_tag="ACHUU3_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR2082121.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14399..15277
                     /gene="dapA"
                     /locus_tag="ACHUU3_002054"
     CDS             14399..15277
                     /gene="dapA"
                     /locus_tag="ACHUU3_002054"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR2082122.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15294..16328
                     /gene="bamC"
                     /locus_tag="ACHUU3_002055"
     CDS             15294..16328
                     /gene="bamC"
                     /locus_tag="ACHUU3_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR2082123.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            16541..17254
                     /gene="purC"
                     /locus_tag="ACHUU3_002056"
     CDS             16541..17254
                     /gene="purC"
                     /locus_tag="ACHUU3_002056"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR2082124.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17422..18270
                     /locus_tag="ACHUU3_002057"
     CDS             17422..18270
                     /locus_tag="ACHUU3_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR2082125.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18285..20300
                     /gene="tmcA"
                     /locus_tag="ACHUU3_002058"
     CDS             18285..20300
                     /gene="tmcA"
                     /locus_tag="ACHUU3_002058"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR2082126.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRGSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20374..21072
                     /gene="ypfH"
                     /locus_tag="ACHUU3_002059"
     CDS             20374..21072
                     /gene="ypfH"
                     /locus_tag="ACHUU3_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR2082127.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21182..21382)
                     /locus_tag="ACHUU3_002060"
     CDS             complement(21182..21382)
                     /locus_tag="ACHUU3_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR2082128.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21410..22537)
                     /gene="dapE"
                     /locus_tag="ACHUU3_002061"
     CDS             complement(21410..22537)
                     /gene="dapE"
                     /locus_tag="ACHUU3_002061"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR2082129.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(22541..22897)
                     /locus_tag="ACHUU3_002062"
     CDS             complement(22541..22897)
                     /locus_tag="ACHUU3_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR2082130.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         5761 a         6089 c         6158 g         4963 t
ORIGIN      
        1 aaatggcgaa tggcaatggc gaacggaatc acaaacaaat tggcgataca gtgctcaaac
       61 ccactggcga caaacagggt gatgggcaaa atcatggcga cgattttgtc gcataatgaa
      121 cgcccgcaat aactcatcca cagcgccaga caaaccatca gattgcacat aatgccgagg
      181 ctgacagatt cagtaaatgt atgatgcatt ttgccctcgg cgcagtgtaa aaccgcgact
      241 ccccacatcg cgttttcact catcaccagc ccggaaaacc agattaacaa actgaaacag
      301 gcaatacctg ccagattacc gcaggccacc agaagtgcgt taatcagcca agttcgccaa
      361 ctaataacgc cccgactttt tgccatcacc gtcattaccg acgaggtgaa caggctggtg
      421 ccacaaaccg ccagcaaaat aaagccgagt gtaaagcaaa ggccgcccac cagatgggtt
      481 aatgcctgtg aagacggggc atcggcgata acggaaaggt aaaaagtaaa tccaatcgcc
      541 ataaacacgc cagcggacat cgccattaaa aagaccgaca ctttgctgcg ggcaatttta
      601 tgggcagtaa tccgttcagc gattgccgct tttctggcgc tcaactgcaa gtcgaaagag
      661 agtttgtttc tcatgcatac tcctgcaaaa gcagattaca acacctcgcg aaccgagagc
      721 cccagacgct gcattcgtga cagcagcgtg gtgcgcttca tccctaatcg tgtcgccgcg
      781 ccacggggcc cggcaacaat gccattggtt tctcgcaata cctgaacaat gcgctgacgt
      841 tcttcttcgt cattctctgg cgtcgtcggg tgcagcaact gcgccattga actgcgaagc
      901 gctgaatctt catttagcgt cggcagtaaa tggctttgtc ggacattaag atgtaaattc
      961 agactgttac cgcgagtcag cagtaccgct cgctcaatca cgttttccag ctcgcgcacg
     1021 ttgcccggcc agtcccacga catcaactgg cgtagcgcct cggtcgggat ggcgtcaatt
     1081 gaacgattca tatggcgcgc cattttttgc gtgaaatgct ttgctaaaag agggatatct
     1141 tccggacggt cgcgtagcat agataatgtc accgacgccc agattcgaaa ggagctgctc
     1201 gctaagctgg tggttttctt gctgcaaact ttcctgcaaa tcggtcatgc tacgccaggc
     1261 atcggcattg ccaacggcaa tagcgatgcg atcggctatg tgttgtagta gctgacagtt
     1321 ttcctcacta aagagagtgg aagaggtatg cgccagcaac aatgcacccg gtgtatggtt
     1381 gccaaaggta agcggtataa ggagcgcaga ttcgcagccg ttagatgcaa gtaagagtaa
     1441 taacgggtcg cgttgccaga gaaacagatc gtctgcacgg tgcgtcaagg tcggttgtcg
     1501 ggtttgtagc gtttgtgtca gcaatacact gtcgccaggc atattgcgtg gcagacacgc
     1561 acaatgtgag gcagaaagat cgctgcacca caggctaaac ttctcgttct ttcgatgatc
     1621 gcccagtacc atactgactg aagccagacc gaaaaaatga tggatctcac gagcgacgtc
     1681 agcgatcaga tcgtcgagat caagatgtga cagcactgca ttagtaatat caaccagtac
     1741 ccgatgatga tcgcgatctt tctccagcaa acgcagcgcc acctgctgtt cttcatcatt
     1801 gagccatgat tgcagaaccg gggtgattat ccgcgccagc cagttaaaca gtgtgcaatc
     1861 ctggggagta aaacggcagc cgtgggtgcg aataaagcgt agctccgcca ttccactact
     1921 gccgagaagg acggcgtgac gcgaataatc gaccatctcg ccctgatact ctgttgttgc
     1981 cgtcacatcg ctgccatcat catggtagcg gtaatagaag ttgcttttga gaacatgcag
     2041 ttcaataagt tgtaccgggc tgaacggcgg cggcagcggg atgaaggcgc gtaataaaga
     2101 cgcgtaataa agacgcctta ccgtgtttct gcgctaaccg ctccgcgagc attccgttta
     2161 ccgcttcaat tgttattcct tgcggcgggg caaacatcgc ctcgtctgac atagccatca
     2221 acagtcctta ttaatgagaa atccttctca cgcaattttt ctggccatca gatagatggc
     2281 cggttcctgc acaatttcat ccaacagatg aatgaattga atgctacgcg cctgccacac
     2341 agacgtcgcg tcatccgcca gtggcgcgaa tgcccgggtc aacagggcgg tgtggctggc
     2401 gtcaataaca atctcaccaa aagtcatcac ccccagcatc ttacgtcggg cttgctcctc
     2461 ttcgaccagt gcaagccaat cttcgtattc cgtcagtggg caggggaagg cgacattcag
     2521 acaatcaatc acgccaacgt gatgaccgat agccagcgag taatacatta cctgagagct
     2581 gtgttccggc atctcgtcgc tactggcgac aaactttttt cgcagcgtcc agaaaacaat
     2641 ttccccgcac tcttcagtca tgtaaccccc ggagtacgcg aaagagttgc tgaacgatac
     2701 tattgagacg tggatcgtcg gcatcgcgca accaggtgtt aacccgattt cctgtaggat
     2761 cgtcgcttaa atggcgcagg agccgatcgc aaatttctcg cccctgacga tagcccgcca
     2821 gccgccgcgc ttctcgctca atggcgatac gctgtgatgg cgggatctgc ggccacaacg
     2881 gttgcatagt caccccggtg ggatcgcgat aatccaccgc gtgaatcttc tgttgcagca
     2941 aaccgagcgc cacggcgaaa ccgtgaatgg tggcggccgg tgttggcggg cagccgggga
     3001 tccaaacatc aatggggaca atggtgtcgc tgccgcccca gacgctgtag agatcgtgga
     3061 aaataccgcc gccgacaccg cacgcgccgt aggaaacaca aattttatga tcgggggcag
     3121 actcatacgc ccgaagtgca ggcatacgca tcgcccgggt gactgcgcct gtaaataaca
     3181 aaatatcggc gtgacgcggt gatgaaataa ccttaatgcc aaaacgttct gcgtcgaata
     3241 ctggtgtgat ggcggcaaaa atttcgattt cacaggcgtt gcagccgccg caatcgacgc
     3301 gataaacgta agccgagcga cggatatcct gtagcaaatg ctgcttcatc ttttgcgttt
     3361 gctcgtccag cgtgatgggc tggctgacat gttgtgtaag cactggactc atagctgctc
     3421 cttagccacc agcggtacat ctgtatcgtc gttgagcagc gtcgcgcgtt gtttgcactc
     3481 cgggcagacg ctcgcttgcg cccataacat ttcgcggttt tgtggcgcat tttgttgctg
     3541 tgccaacaat tcagcagcca gtgcgacggt tttttgcggg gcaaacgggc gttcgcaacg
     3601 gctgcaacgt tgtagatgga acgtcgcgcg ggtatagaga tcggctttat tggtgacggt
     3661 cagttcaaag ttattggtaa gctggatggc tctggtcggg cacacttctt cacaacgtcc
     3721 gcagtaaata caacgcctca gatagagctg ccaggtgcgc gaattttgct ggtcgtcggt
     3781 ctggatagtc agcgcatttg ccggacaagc acaggcgccg caggcaatac attggctggg
     3841 catcaggtcc ggtttcccgc gaaagcctgg gctgacctcc agtggcgcga agggatattt
     3901 caccgtcgcg gttccggcgc gcataatagt tttcagtaac ttcagcatct tctgtcctta
     3961 cttcagcggc gaacggttac gatcaatgcc gtagcgttcg atctctttat acggcacggt
     4021 ttttgactgg cgcttgcgca catctaccag cgtcacacgg tcggtacagg agtagcaggg
     4081 atcaaggcta ccgataatca gcggtgcgtc agaaacggta ttgccgcgca gcatgtaacg
     4141 caacaccggc cagttggcgt aggtggcggc acggcagcgc cagcggaaca atttttggtt
     4201 atcaccgagc atgctccagt gcacgtcttc accgcgtggc gcttcaacaa agcccagcgc
     4261 gaatgcgtga ggtttatagc taaagccttc ggtcagcagt ggggtatccg gcatgttgtc
     4321 gagggcaaat tccagcattg ccagcgaatc aaacgtctct ttgacacgga ccatcacgcg
     4381 ggagaaaaca tcgccgccgg taaaggtaaa cagtgttttt ggaatattgc cgtagtcggc
     4441 gtaggggtga tcaaagcgca aatcacgggc aaaaccactg ccgcggatca gcggccctac
     4501 agggctataa tcacgggcga tttgtcggtc gagaatgcca atgccctgag tgcgttgttc
     4561 catattcggc gtggcaagca gcatctctac cagctccgac acgtcggcgc gcatctcgcg
     4621 caccagtttc agcgtttgca gacgttgctc tttgagaata tcgcggcgaa caccaccaat
     4681 cagattcaga ccgtaggttt tacgcgaccc ggtcagcaat tccgccattg tcatcgactt
     4741 ttcccgcacg cggaaaaatt gcataaagcc ggtatcaaat ccaacaaaat ggcaggagag
     4801 gccaaggttg agcaaatgac tgtgcagacg ttcgacttcc agcagaatcg agcgaatagt
     4861 atgcgctcgt tgcggcacct caatccccag tgcattttca accgagttgg tataggccac
     4921 actgtgggca aaaccgcaaa tcccacacac gcgatccgat aagaaggtca cttcgttgta
     4981 gcccatccgc gtttctgcca gtttctccat gccgcgatgg acataaaaca ggcggtaatc
     5041 agcatcgaca atttgctcgc catccacaaa caagcggaag tgacccggtt catcggaggt
     5101 gatatgcagc gggccaatag ggatcacccg cgcatcgcta ttgccctcat tgataaacgg
     5161 atacgtttcg gtatcagtcg tcggttcagg gcgcagtcga taatccatcg catctttgcg
     5221 cagcggatgc atatcttccg gccagtcatc gggcaacacc aggcgacgct gatccggcag
     5281 gccaaccgga atcagcccgt acatatcgcg aatttctcgc tcgccccaga ccgcggcagg
     5341 gacgcgcggt gtgactgacg gaaactcccg actgtcggca tcgaccagtg ccttcaccac
     5401 gatccagcat ttttcggccc cttccattga aagggcataa taaaccgcgt aatgaccgtt
     5461 aagtgtccgc tcgtcgttgc caaacaggac tggaagccag ccatcatgtt gataataaag
     5521 ataatgtaca acgtcaggca gcagattgat tttcaccgta atggtgacct gttcaggcgt
     5581 ttgtcgctct tcatccagca ccgcgccggg gaactgcgtt ttcagggcgg cgagaatcgc
     5641 ttcgccacga tttgacgatg aattaacgtt cactgtgttt ctccgaaaca gatgcggtgc
     5701 ctgaaggcaa aaaatcatgc caggtgctac gttgtgcagg caggtcgtgc gtccctgaga
     5761 ggactatagt ggaagcgccc gccaggatcc tgatgacagg ttgtggaata tgcgttccca
     5821 tcgccagcat catgaccagc agaatcacca ttggcgaggt ggtcaaccag ccgagatcac
     5881 cccggttaac ggcctgcggc ggtttcgcca ttaacacccg cgctgccatc cgtaccaggc
     5941 ccgccagcac cagcgttaac agcaataaca gcaggacgat aatcagcagg tgattacgtg
     6001 ccagtccggc ggtaatggtc ataaattcgc taagaaaaat gttgaagggc ggcatccctg
     6061 ccagcgccag cgcaccgccg ccaaacagca cggcggtaaa tggcatgatt ttgagcatcc
     6121 cacagacgac gttgagatcg cgcgtgccgt acttgagcag tacattgccg gaaccgcaga
     6181 acagcagcgt ttttgccaga ctgtggttta agatgtgcag cagcgcggca aaaattccca
     6241 gcggcccgcc aatgcctagc gccaccgcga ccagccccat gttctccacg ctggagtacg
     6301 ccagcagacg cttaatgtcc cgctgtacca gaatgaaaaa tgccgccacg gcaaccgaca
     6361 acatgccgaa gatgagcaac aaccggttgg ggaaatcgct gccgatggct tggcaaataa
     6421 tgatgtaata gcgaatcagc accaacagcg cgcagttcag caatacggcg gagagcaggg
     6481 cgctgaccgg actcggcgct tcactgtgag catccggcag ccaggcgtgc atgggaaata
     6541 gcccggtttt ggtaccaaag ccaattagca caaacacaaa ggccaacagc attaatgttg
     6601 ggtcaagcaa ggacgattgc ttaagaacct cgctccagaa tatcgccatt tctgcctgcg
     6661 gcataacgct ggcggcgttg gcgtatacca gcacggtacc gaacagacca aaagcgacac
     6721 caacagtaca aataatgatg tacttccatg ctgattccag cgatgaacgc tgaccgtaaa
     6781 tgcctaccag aaacgccgag cttaaggtgg tggcttcgat cgccgcccac atcacaatca
     6841 ggttattgct ggtaacaacc agcagcatgg tgaacaaaaa cagatggaag aaaccgtagt
     6901 aatcgcacag cgtaacgggt gaaagctcgc cgtgtgccac ttcatgacgc atgtagccaa
     6961 tcgagtaaac accggtgaga aagccaatca caccaagaat ggcgaggaac aaaccgccca
     7021 gaccatcaat atgaagccac agtcccgcag cgaatatttc tcctgcatca gcggcagttt
     7081 ggaccaccca gagtgccaga atcagcagca gtgtgatccc taagctatgt aatacagtca
     7141 ccgtacaagt cgcagagagc ccccgtttcc ggcaggcaaa acagagcagc gaaaaaagca
     7201 gcggcgtgag caggagtaaa gcgaacatca cagaataact catctcatta tcccttcagc
     7261 gcggtcaagt tgttcacgtc cagcgtgccg tgggtacgcc atatttttct tgccagtaac
     7321 accatcataa tgacggcgaa gatggcgtcg gtagcgatac ctatttccac cagttccggt
     7381 gctcgccagg caagaagcgc cagcaccaga tgggagccgt tttccatcag gcagtaacca
     7441 aaaatttgcc gcaggatatt gcgctggctg acaatgcaca gcaggccaag cagaaaatga
     7501 cctaacgcta ccgccagcgc cggtttcagc ccggtagcca tcggtagctt cacgggctga
     7561 acgacaaatg cgcaaagcag gacaattaac gccgcgagca gtgccatcat tgccggaccg
     7621 aataacgctt tttccgggat gttctgggga atatttcgtg cagcgtaagt catgattaac
     7681 ggtaccagca gcactttggt gataaaggcg ctggcggacc agatcagcag ttgctctgcg
     7741 gcgaacaggc acgagagagt ggcgaaaata gacaccagca ccagcgactg gcaggcgtaa
     7801 aatccgcatg acaggcgata gcttttgacg ctaatcacaa acagcgatgt cagcatcatc
     7861 agtcccgcca gattatttac gatcatagaa ccggtcattc atcatctcct tactgcaacc
     7921 aggtcgcggc gattacgctg gaaatcagcg acatcacaat cagtacaatc aacaccaggc
     7981 gcattgaccc tggcagcggt gcggcatcgg cgacggcctc gctcggtttg cccggcacga
     8041 cgcgaccaaa ccagcgaata aaccaggcga aactggcgac cgattcaatc atcagcagaa
     8101 tcatggcggg cagcaggatc cagtactcca ctgacaacgc aaaaccgacg gcaaacagcg
     8161 ggaatttact gaagaagccg ttgaacggcg gcacaccggt aatcgccagc gctgccacgc
     8221 agaaacccac gcttggcaac ggcagggtgt gcaatacgcc acgcagacgc ggcaacaagc
     8281 gcgtgccgca gctgtaactc agcgcacctg ctacaaggaa aaacaggctt ttagcgaacg
     8341 cgtggttgac gatgtaggcg atgctaccct ccagcgccag ccgcgagccg aagatggaga
     8401 gcgacaagcc gaagaacatc cagccaagtt gagtgatggt cgaccaggcc agcaaccgct
     8461 tcatatcctg ctgtggcaaa tacatcagaa agccataaag aatggtgacc agcgccatga
     8521 ccatgccaac gccgccaatc acatgcggga tattgccgcc gtcgataatg gcgcgggcaa
     8581 aaatgtaaac gcccactttc accatcgatg cggcgtggag ataggcactg atcggtgttg
     8641 gcgcttccat tgcatccggt agccacgctt gcatcggtag ctgggccgat ttcccccacg
     8701 cggcaaacag gatgccgcca taaaccagat aacgtgcgtc gccgtgtaac tcgctcatcg
     8761 cgctaagcgc aaacgttccg gtctgcaaaa acagcgtggc ggcggcaaga tacaacccca
     8821 acgagccgat atgagtgata agtaacgctt ttagtgctga aagctgtgct ttatcgctct
     8881 ggtaataact gatcaacgcc caggagcagc cgcccgtaat ttcaaaaaac aacaactgac
     8941 cgagcagcgt cgaggagagt accagtcccg ccatcgcgcc gataaacacc agcaaaaatg
     9001 cgtaataacg attcgtgccg ttatgcgggt gttcgcgatt tttatccgtc agataacccg
     9061 tggagtagat cgtgaccagc aaaccgagaa acaccaccac aaacagaatc agcgtactca
     9121 tgcgatcaat gaccaagcca aacaatgcca catcccccac gttgaccaac gtcaacgtga
     9181 cggcaacttt atcggcctga taaaacgcgg agatcagcga caacatgcac agcgtggtca
     9241 gtgcggcgaa caaaaccccc attcggcggc ccgacgttgt ggcgaaaacg aaacgaccag
     9301 tgcgccgata aaaggcagca ttaacgtcgt cagagcaaga ttttccatat tccgtcagtg
     9361 ctccttacag accggttaac cagaagaccc atgcaagcgc agcaaggctg aagccaagcc
     9421 aggtcacatg gtgaatgagt aaaaaacgcc cgcgtgccag cgtgttttct gcgattgagg
     9481 ccagtacaaa aatcagcaaa accttgagca gcgtaacgac aagtgaagtc agcaggcagg
     9541 cgagagaaag ttcttgcgcg cgcccaaagg gcagaaacag ggccacaaac agtgctgcca
     9601 tcacgacctg tttcagcccc agcccccatt tcgctagcgc cagcccggca ccggaatatt
     9661 cggtcagcgg gccttcctgt aattcctgtt ctgcttcagc aacatcaaag ggaatttttc
     9721 ccatctcaat gaagcaggca aaaccacagg ccagtaacgc cagtacggtg gttagcggcg
     9781 agttccagcc cgttgccagc gtattgctga tcatctcgat atgcgtggaa cctgctatca
     9841 gcgccaatac cagcagtgag agaatgagca tgggttcgac cagaatgccg agcgtcaact
     9901 cgcgactggc accgactccc gcaaacgggc ttccggtatc cagccctgaa agagcaaaga
     9961 aaaaacgaaa cagggcaaga agatagataa gggtgatcag atcgccgccg cccgcaaaag
    10021 gggaaacggt aataaatagt ggtagggcca tcgccagcac cagcatgctg ctgattaata
    10081 cccacggcat caggcggaac atcagacctg aagatgtcgg cgcatcttcc tggcgtttaa
    10141 acagtttgtg gatatcgcga taatcctgcc agatccccgg cccgcggcgg gagtgcatac
    10201 gcgcgcgtat ctgccgggaa atacccgtaa aaagtggggt tagcatcagc agaatcacgg
    10261 cctgtgctaa cgcaaaaatg accagatatc cgtcgcaaag agtttgtctc atggtgattt
    10321 ccttagacgg caatagcgat tagcagcaca accagtgcgg cgaccacata caggcaatag
    10381 agacgaaagt cgccgctttg cagatgctgt atttctttgg ccagccgttg ggtggcgctc
    10441 acgatggggc ggatcacccg ctcatcccag aagggttctg tgctctgagc cctggcggtg
    10501 acgtgcgcaa gacctttatt tagcctcagc gtagggtcga gctgttgtcg tagacgaaat
    10561 agcgcagaaa agaccacacg cagcggctgc atcacgccat tgcctgacgg ggccatcgca
    10621 ttttcccagc cgtagccgca tgcccaggca tctcctgtgc gacgaaacgc tgcgcggcgc
    10681 gaacgacacc acagccagta aaggccaggc agcaaaggca tcgccagtag taacagcaac
    10741 agtaatgaag gagtgacccg tgtatgaaac gtgccgggta caagtgctat tccgctggcg
    10801 acagtgacgg gaggggtatt ggtaaacgca tgggcaatgt gcattatctt cggtgccagc
    10861 caactggcac taagcgcaat taatacgcag agtgccgcga gcagtagcat ggcgacgatc
    10921 atcgtatttg gcacttcctg tgcctcttca gcgtgtgtgc tgcgcggcgc accacagaaa
    10981 gtaataccgt acattttgac gaagcacact actgccagcc caccagtgac tgccagcatt
    11041 accatagcaa taggacccgc aagttgtagc gctacggctt ccacacgact tagtgagaat
    11101 agcgactgcc aggtgtacca ttcgctgata aaaccattca gcggaggaat ggctgatatc
    11161 gccaggcaac caatcaggca tgctgcggct gtccacggca tccgtttcgc cagtgccccc
    11221 attttttcca tgtcgtgggt atgcaaacgc gaaataatcg cacccgcgcc gagaaatagc
    11281 agccctttga aaagcgcatg gttgagcaga tgaaacagtg cgccgagcag tccaaccacg
    11341 gtgagcagcg ggtcgtgcag tgacagaccg accatcgcca caccgaccgc cagcaaaata
    11401 atgccgacgt tttcgacggt actccaggcc agcagccgtt tgatatcctg ttccgccagt
    11461 gcatacagca cgcccaggag cgcggagatt gcgccgatcg ccatcaccag aatgccccac
    11521 cacagcggca aacccgtttg cgccagcaga tccatcgcca ctttcaggat gccgaaaata
    11581 ccgattttga ccattacgcc agacatcaac gctgaagcgt gcgatggtgc ggcagggtga
    11641 gcgcgcggca accagctgtg caacggcatc atcccggctt tcgcgccaaa accgaaaaag
    11701 gccagcagga acaccgccga cgccagcccc ggagaaagtg aaagcgtgcg gaaactggca
    11761 aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcattat cagcaccgat
    11821 ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga tcgacgtttt atcctgcctg
    11881 gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat gaaccaaaaa
    11941 gcgttgtcca tcaccagcag ggcaaccatc gatgtgatga aaatattcat aaagaagccc
    12001 atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata gagagaacaa
    12061 accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc cattcgcagg
    12121 gttaaatcgg caaattcaaa aggcgtggca aaacgggcga ctaaagttac gcctgcatgt
    12181 aattgcgtaa ttccgctgat gatgccaatc acgccgccta ccagcgatgt gatgccggaa
    12241 agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact ggcaaacaga
    12301 tagaggatca gcgaccaggt taataattgc agggcatcca ttagcgttgc tcctgagtga
    12361 gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc tgtttttctt
    12421 tcatctggcg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc gggcaagcgc
    12481 gaacgcaggc cggaccttct ggcaagaaat cacacaggtc gcatttcacc gcgacggtct
    12541 ggacacccgg ttcccagcgc agcaacgcat gttggggcgg cacgttttct tcgccgtctt
    12601 tgagtgagcc ctcagcctga aaaacatatt gcgcaggggc attcaccgga cggcttcctg
    12661 aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca atacagaggc
    12721 tttcgttgag ttggatcgca tcatccctct gagagatggc attgaccggg cagacctgca
    12781 ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgagtgtt gacgtcttcg
    12841 ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc gaacaggcag
    12901 cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag cggttcatgt
    12961 cggctccttg aaaatgatat gaaacattgc atttcatatt gcataaagga tgccattctg
    13021 attaactcaa tatattcaat aagttatgta ttaactggat tctgaacggt acaaaaatga
    13081 tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg aaagcgagaa
    13141 tgtgatcttg atcacgagaa gtgaggtatg ctggccgcaa gcgcagccag cacggaatgg
    13201 agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg tgattgctgg
    13261 ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg aagctgatgc
    13321 gatgaatgcc atcgtaggtt ttgcccatga aggacttttc accccagacg ccgaattgtt
    13381 cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct ttttccgcaa
    13441 aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg cccgcttttt
    13501 tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg ggggtcatgg
    13561 ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa ttaacttgtt
    13621 ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc agtggattca
    13681 ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa tactgccttg
    13741 tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg cgtccgcaga
    13801 tgcggggctg tgggcggtta tctgaatatg caactgcgca gcccgttcat tttcagcagg
    13861 ttgcgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa gtgctgtgaa
    13921 gcgttcaatt aaatgcgggg agtctgccac atcgacctga acccagacag atgctggcat
    13981 tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca gctcggcacc
    14041 tttcaacggt aacgttgatt caatcagagt aatggcattc catgaaccgg aaagcagcat
    14101 gataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat tacagccgca
    14161 actactgaca tgacgggtga tggtgttcac aattccaggg cgatcggcac ccaatgcagt
    14221 gatcaccaga taatgttgcg atgacagtgt caaactggtt attcctttaa ggggtgagtt
    14281 gttcttaagg aaagcataaa aaaaacatgc atacaacaat cagaacggtt ctgtctgctt
    14341 gcttttaatg ccataccaaa cgtaccattg agacacttgt ttgcacagag gatggcccat
    14401 gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta atgtctgtcg
    14461 ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg cgatcgtttc
    14521 tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg atgtggtgat
    14581 gatgacgctg gagctggctg atgggcgcat tccggtaatt gccgggaccg gcgctaacgc
    14641 tactgcggaa gccattagcc tgacgcagcg cttcaatgac agtggtatcg tcggctgcct
    14701 gacggtaacc ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc atttcaaagc
    14761 catcgctgag catactgacc tgccgcaaat tctgtataat gtgccgtccc gtactggctg
    14821 cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta tcggaatcaa
    14881 agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt cagatgattt
    14941 tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattag gcggtcatgg
    15001 ggttatttcc gttacggcta acgtcgcagc gcgtgatatg gcccagatgt gcaaactggc
    15061 agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc cattacacaa
    15121 caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg aactgggtct
    15181 tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg gtcgtgagac
    15241 ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat ttgatggctt
    15301 actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt ttattactcg
    15361 ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa gcctacctgg
    15421 aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg gtgacctccg
    15481 gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg ctggacattc
    15541 gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc acgggcgata
    15601 ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag gtggttagcg
    15661 tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag acactgacca
    15721 ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt cgttatcaaa
    15781 tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgttg aacctggaac
    15841 aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg gagatgatga
    15901 acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct gcgcaaaatc
    15961 gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta ccaatgctgg
    16021 tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcggcgctg gaaaaagtgg
    16081 gcatgaaagt gaccgacagc acccgttcgc agggcaacat ggccgtaact tataagccgc
    16141 tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca tccggtgact
    16201 ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc gatccgaaag
    16261 gtcatactct gactcagagt cagaacgacg cgctggtagc tgtcttccag gctgcgttta
    16321 gcaagtaaaa atacagggct ggaatcatcc ggcccttttt tctgatatga tacgcaaacg
    16381 tgtgcgtcgg caggaaaacg cgattttagc ggtaattcgc acgaaatttg tttgtcggac
    16441 gtagttcgga taaggcgttc acgccgcatc cgacaaaaca tccggcacac cagacagcaa
    16501 aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc aagctgagtt
    16561 gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt tggtgctcga
    16621 attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg atcgcaaagg
    16681 tatgttgaac aacaagttca actacttcat tatgagcaaa ctggctgaag cgggtatccc
    16741 gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc tggatatggt
    16801 gccggttgag tgtgtcgtgc gtaaccgtgc tgcgggctct ctggtgaaac gtcttggaat
    16861 cgaagaaggt attgagctga acccgccgct gttcgatctg ttcctgaaaa acgatgccat
    16921 gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga gcaaagagaa
    16981 cctggcgcgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa aactgttcga
    17041 tgatgctggt ctgattctgg tcgacttcaa gctggagttt ggtctgtaca aaggtgaagt
    17101 ggtactgggc gatgagttct ccccggacgg cagccgcctg tgggacaaag aaacactgga
    17161 gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgaag cctatgaagc
    17221 cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc tgttcatcat cttgccgtgc
    17281 agctggcacg gcaagacaac cgctctcgga atatatgcat tgaacgctca ttcctccgcc
    17341 atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca tttctataat
    17401 gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga caatgtcgaa
    17461 gacaggcgta acagctccgg cggtccgtca atgggcggtc ccggttttcg cctgccaagc
    17521 ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta tggtgttgat
    17581 ttaaccgggt tgatgaccgg acagccggtt tcccaacagc aatcaacacg gtcaattagc
    17641 ccaaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac ggaagacacc
    17701 tggggacagc aattcgagaa gatgggtaaa acctatcagc aaccgaagct ggtcatgtat
    17761 cgtggaatga cgcgtaccgg ctgcggggcg ggccagtcca ttatgggacc gttctattgc
    17821 ccggcggatg gcacggttta tatcgatctc tccttctatg atgacatgaa agacaaactc
    17881 ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg tcatcatgtg
    17941 cagaaattgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc gacgcaggcg
    18001 gaagtaaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc cggtgtctgg
    18061 gggcataaca tgcagcagca aggcgatctg gaagaggcgc tgaacgcggc gcaggccatc
    18121 ggcgatgacc gtttacaaca gcaaagtcag gggcgagtag taccagacag tttcactcat
    18181 ggtacttctc agcaacgcta cagctggttt aaacgtggtt tcgacagcgg cgatccggca
    18241 caatgcaata cttttggtaa aagcatttaa cttccggggc aaggatggct gaactgactg
    18301 cgcttcacac attaacagcg caaatgaaac gtgaagggat ccgccgcttg ctggtgttga
    18361 gcggggaaga gggttggtgt tttgatcatg cgcttaagtt gcgtgatgcc ttacctggcg
    18421 actggctgtg gatttcgccg cagccagatg ctgaaaacca ctgttctccc tcggcgctac
    18481 aaactttact tgggcgcgag ttccggcatg cggtattcga cgcccgccac ggctttgatg
    18541 ccgctgcctt tgcggcactt agcggaacgt tgaaagcggg aagctggctg gttttgttac
    18601 tccctgtatg ggaagagtgg gaaaaccaac ctgatgccga ctcgctgcgc tggagtgatt
    18661 gccctgaccc tattgcgacg ccgcattttg tccagcatct caaacgcgta cttacggcgg
    18721 ataacgacgc tattctctgg cggcaaaacc agccgttctc gttggcgcat tttacccccc
    18781 gtactgactg gcaccccgcg accggcgcac cacaaccaga acaacagcaa ctcttacagc
    18841 agctactgac catgccgccg ggcgtggcag cggtaacggc tgcgcgtggg cgcggtaagt
    18901 ccgcgctggc agggcaactc atttctcgta ttgcgggcag tgcgattgtc accgcgcccg
    18961 caaaagcggc aacggatgta ctggcacaat ttgcgggcga gaagtttcgc tttattgcgc
    19021 cggatgcctt gttagccagc gatgagcaag ccgactggct ggtggtcgat gaagccgcag
    19081 ccatacctgc gccgttgttg catcaactgg tatcgcgttt tcctcgaacg ttgttaacca
    19141 ctacggtgca gggctacgaa ggtaccggac gtggtttttt gctgaaattt tgcgctcgct
    19201 ttccgcattt acaccgtttt gaactgcaac agccgatccg ctgggcacag ggatgcccgc
    19261 tggaaaaaat ggttagtgag gcactggttt ttaacgatga aaacttcacc catacaccac
    19321 aaggcaatat cgtcatttcc gcatttgaac aaacgttatg gcgaagcgat ccagaaacgc
    19381 cgttaaaggt ttatcagtta ttgtctggtg cgcactaccg gacctcgccg ctggatttac
    19441 gccggatgat ggatgcacca gggcaacatt ttttacaggc ggctggcgaa aacgagattg
    19501 ccggggcgtt gtggctggtg gatgagggcg gattatctca agaactcagt caggcggtat
    19561 gggcaggttt tcgtcgcccg cggggtaatc tggtggccca gtcgctggcg gcgcacggca
    19621 gcaatccact ggcggcgaca ttgcgcggac ggcggggcag ccggattgca gtccatccgg
    19681 cgcgtcagcg cgaaggcgtt gggcaacagc tcattgccag cgctttgcaa tataggcctg
    19741 gcctcgacta tctttcggtg agttttggtt acaccgggga gttatggcgt ttctggcaac
    19801 gctgcggttt tgtgctggtg cgaatgggta atcatcgtga agccagcagc ggttgctata
    19861 cggcgatggc actgttaccg atgagtgatg cgggtaaaca gctggctgaa cgtgagcatt
    19921 accgtttacg tcgcgatgcg caagctctcg cgcagtggaa tggcgaaacg cttcctgttg
    19981 atccactaaa caatgccgtc ctttctgacg acgactggct tgaactggcc ggttttgctt
    20041 tcgctcatcg tccgctatta acgtcgttag gttgcttatt gcgtctgcta caaaccagtg
    20101 aactggcatt accggcgctg cgtgggcgtt tacagaaaaa caccagtgac gcgcagttat
    20161 gtaccacact taaactttca ggccgcaaga tgttactggt tcgccagcgg gaagaggccg
    20221 cgcaggcgct gttcgcactt aatgaggttc gcactgagcg tctgcgcgat cgcataacgc
    20281 aatggcaatt ttttcactga ctccttcaat tttctcccat ggtcattgtg agtcagcggc
    20341 gtaaaattaa tgacatcagt taaggagttc gccatgaaac atgaccattt tgttgttcaa
    20401 agcccggata aacctgcgca acagttgtta ctgcttttcc acggtgtcgg ggataacccg
    20461 gtggcaatgg gggaaatagg cagctggttt gcgccgctat ttcctgatgc gctggtggtg
    20521 agtgtcggag gcgcggagcc gagcggtaat ccggcggggc gtcagtggtt ttcggtgcag
    20581 ggtattacgg aagataatcg ccaggcgcgt gtggatgcca tcatgccgac gtttattgag
    20641 atggtgcgct actggcagaa acagagcgga gtaggggcta acgccacagc gctaatcggt
    20701 ttctctcaag gcgcgattat ggcgctggag agcattaaag ccgaaccggg ccttgcttca
    20761 cgcgtcattg cttttaacgg acgctatgcg agcctgccgg aaactgcgtc tacagccacg
    20821 acaattcacc tgattcatgg cggtgaagat ccggttatcg atctggcgca tgcggttgcg
    20881 gcgcaggaag cgttaatcag tgccggtggt gacgtgacgc tggatatcgt ggaggattta
    20941 ggccatgcga tcgacaatcg cagtatgcaa tttgccctgg atcacttgcg ttataccatt
    21001 ccgaaacatt atttcgacga agcgctaagt ggcggtaagc ctggcgatga tgatgtgatt
    21061 gaaatgatgt aaaagacaga ttacctggcc tgataagacg cgtgagcgtc gcatcaggca
    21121 tcgttcacca ttgccggatg cggcgttaac gccttatccg gcctacggtt gagcactcag
    21181 cttacttctt cggttgatcc tttttcggcc agtcgtcgtc atcgtcccac ttatcgttga
    21241 aatcacgatg cgggggaagc tccggcttgt tggcgagaaa tttcttgtgg tcgacgcgct
    21301 tgagatcttt aatcacgttc agcaggacgc ctaccaaaaa gacaatcacc agaatccacc
    21361 aatatttagc cagccagtcc atgcttattt cctcttgcag aaccactcat caggcgacga
    21421 gctgttccat gatacgttga tacatacggg caagtagctg caggtcggca gcgttcacac
    21481 attcattaat tttatgaata gtggcattga ccggcccgag ttccaccacc tgcgccccca
    21541 tgcgagcaat aaagcgcccg tcggacgttc cgcctgtggt cagtagctgc ggtttaattt
    21601 cattatagtg ctcaaccgcg ttaacgaccg catccaccag tttaccgcgc gcggtcaaaa
    21661 atggctgccc ggaaagccac caatccaccg tatagcgcag ttgatgtttt tcaagcaggg
    21721 caagcacctg cgctttgatc atctcatcag tcagttcggt gctgaagcgg aagttaaact
    21781 gcacaaacag ttcacccgga ataacgttgt tactgcccgt tcccgcctga atattggcaa
    21841 tctgcatact ggtcgccggg aagaattcat tgccctgatc ccactcaata gccactaatt
    21901 cattaaggaa aggtgctgcg cgatgtactg gattgtcagc cagatgtggg taggccacat
    21961 gcccctgcac gccatgaatg gtaaggttgc aggttaacga tccgcgacga ccatttttca
    22021 ccacatcacc aaccacttcg atactcgacg gttcgccgac cagacagtaa tcgagacgct
    22081 cattacgcgc cattaacgct tcgacgactt ttaccgtacc gttgtgggca ctggcttctt
    22141 catcagaggt gatcagaaat gccagtcgtc ccgtatggtt gggatgttgt gcgacaaaac
    22201 gttctgctgc taccaccatc gccgccagcg agcctttcat atctgccgca ccgcgcccga
    22261 ataacatgcc atcacgaatg gtgggttcaa acggcggatt gatccaacga tcggcgtcgc
    22321 caggcggcac cacgtcggta tgcccggcaa aggccaatgt ttcaccttgc ccacgccatg
    22381 cccaaaaatt ctgcgtatcg gcaaagtcca tgcgttcaac ggtaaaaccg atcgcctgca
    22441 aacgatcaat caacaaagcc tggcatcctg cgtcatcagg actcagggaa gggcggcgaa
    22501 taagctgttg tgtcagctca ataaccgggc acgacataga ctacacctca tggaaaaatt
    22561 gctgataact ggaatcactg aaacccagca gcataggctt accgggcgcg cagagcaatg
    22621 gacgtttgat aattgcaggc atttcagtca ttaatgccgc cgctgaggcc gcatcggtga
    22681 ttttattgcg ggtggtttcg tccagtttac gccaggttgt accacgggtg ttgagtaacg
    22741 cctcccaacc taattcgttg ataaaaccgt tcaataattc gctgtccagc ccatcgacgc
    22801 ggtaatcatg aaaacgatag tcgatgttat tggcttccag ccaacggcga gcctttttaa
    22861 tggtgtcaca atttttgata ccgtaaagtg taaccataat gaatcctttt tcgatttatg
    22921 agaataaata ctcatttaag ggtgaaaaca ttttgcctgc aaatgattat c
//
LOCUS       DAZPLI010000055        22592 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZPLI010000055 DAZPLI010000000
VERSION     DAZPLI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22592
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.055"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="ACHUU3_002063"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="ACHUU3_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR2082131.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="ACHUU3_002064"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="ACHUU3_002064"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR2082132.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="ACHUU3_002065"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="ACHUU3_002065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR2082133.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="ACHUU3_002066"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="ACHUU3_002066"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR2082134.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="ACHUU3_002067"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="ACHUU3_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR2082135.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6233..7879
                     /gene="mqo"
                     /locus_tag="ACHUU3_002068"
     CDS             6233..7879
                     /gene="mqo"
                     /locus_tag="ACHUU3_002068"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFR2082136.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8188..8697)
                     /gene="eco"
                     /locus_tag="ACHUU3_002069"
     CDS             complement(8188..8697)
                     /gene="eco"
                     /locus_tag="ACHUU3_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR2082137.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(8811..8975)
                     /gene="yojO"
                     /locus_tag="ACHUU3_002070"
     CDS             complement(8811..8975)
                     /gene="yojO"
                     /locus_tag="ACHUU3_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR2082138.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            9084..9578
                     /gene="napF"
                     /locus_tag="ACHUU3_002071"
     CDS             9084..9578
                     /gene="napF"
                     /locus_tag="ACHUU3_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR2082139.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9568..9831
                     /gene="napD"
                     /locus_tag="ACHUU3_002072"
     CDS             9568..9831
                     /gene="napD"
                     /locus_tag="ACHUU3_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR2082140.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            9828..12314
                     /gene="napA"
                     /locus_tag="ACHUU3_002073"
     CDS             9828..12314
                     /gene="napA"
                     /locus_tag="ACHUU3_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR2082141.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            12321..13016
                     /gene="napG"
                     /locus_tag="ACHUU3_002074"
     CDS             12321..13016
                     /gene="napG"
                     /locus_tag="ACHUU3_002074"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR2082142.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            13003..13866
                     /gene="napH"
                     /locus_tag="ACHUU3_002075"
     CDS             13003..13866
                     /gene="napH"
                     /locus_tag="ACHUU3_002075"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR2082143.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            13863..14312
                     /gene="napB"
                     /locus_tag="ACHUU3_002076"
     CDS             13863..14312
                     /gene="napB"
                     /locus_tag="ACHUU3_002076"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR2082144.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            14322..14924
                     /gene="napC"
                     /locus_tag="ACHUU3_002077"
     CDS             14322..14924
                     /gene="napC"
                     /locus_tag="ACHUU3_002077"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR2082145.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            14937..15560
                     /gene="ccmA"
                     /locus_tag="ACHUU3_002078"
     CDS             14937..15560
                     /gene="ccmA"
                     /locus_tag="ACHUU3_002078"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR2082146.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            15557..16219
                     /gene="ccmB"
                     /locus_tag="ACHUU3_002079"
     CDS             15557..16219
                     /gene="ccmB"
                     /locus_tag="ACHUU3_002079"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR2082147.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            16261..16998
                     /gene="ccmC"
                     /locus_tag="ACHUU3_002080"
     CDS             16261..16998
                     /gene="ccmC"
                     /locus_tag="ACHUU3_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR2082148.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            16995..17204
                     /gene="ccmD"
                     /locus_tag="ACHUU3_002081"
     CDS             16995..17204
                     /gene="ccmD"
                     /locus_tag="ACHUU3_002081"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR2082149.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            17201..17680
                     /gene="ccmE"
                     /locus_tag="ACHUU3_002082"
     CDS             17201..17680
                     /gene="ccmE"
                     /locus_tag="ACHUU3_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR2082150.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            17677..19620
                     /gene="ccmF"
                     /locus_tag="ACHUU3_002083"
     CDS             17677..19620
                     /gene="ccmF"
                     /locus_tag="ACHUU3_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR2082151.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            19617..20174
                     /gene="dsbE"
                     /locus_tag="ACHUU3_002084"
     CDS             19617..20174
                     /gene="dsbE"
                     /locus_tag="ACHUU3_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR2082152.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            20171..21223
                     /gene="ccmH"
                     /locus_tag="ACHUU3_002085"
     CDS             20171..21223
                     /gene="ccmH"
                     /locus_tag="ACHUU3_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR2082153.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(21258..21905)
                     /gene="narP"
                     /locus_tag="ACHUU3_002086"
     CDS             complement(21258..21905)
                     /gene="narP"
                     /locus_tag="ACHUU3_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR2082154.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            22225..>22592
                     /locus_tag="ACHUU3_002087"
     CDS             22225..>22592
                     /locus_tag="ACHUU3_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR2082155.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         5055 a         5670 c         6428 g         5439 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat catatttgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatggc gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaatatt
     6061 ctaaaaatcc cttcatttct aataacccta taattaatca acgaaattat aatgtttcta
     6121 aaattagaat ataatttata aacattattt aaatgttgtt actttagtgt taccgttgat
     6181 gccgcgcaaa tctcacctgc aataaagcga ctaaaagtaa ggcattaaca agatgaaaaa
     6241 agtgactgcc atgctcttct cgatggccgt ggggcttaat gccgtttcga tggcggcaaa
     6301 agcgaaagcg tccgaggagc aggaaactga tgtactgttg attggcggcg gcattatgag
     6361 cgccacgttg gggacctatt tacgcgagct ggagcctgaa tggtcgatga ccatggtgga
     6421 gcgcctggag ggtgtcgcgc aggagagttc gaacggctgg aacaacgccg gaaccgggca
     6481 ttctgcactg atggaactga actacacccc gcaaaacgcc gatggcagta tcagtattga
     6541 aaaagcagtc gccattaacg aagcatttca gatttcccgc cagttctggg cgcaccaggt
     6601 cgagcgcggc gtgctgcgta ctccgcgttc atttatcaat accgttccgc atatgagctt
     6661 tgtctggggc gaggataacg ttaatttcct gcgcgcccgt tacgccgcgt tgcaacaaag
     6721 ctcgctgttt cgcggtatgc gttactctga agatcacgcg cagatcaaag agtgggcacc
     6781 gttagtgatg gaagggcgcg atccgcaaca gaaagtggca gccacgcgta cggaaattgg
     6841 taccgatgtg aactacggcg agatcacccg ccagttaatt gcttccttgc agaagaaatc
     6901 taacttctcg ctgcaactca gcagcgaagt ccgcgcccta aagcgtaatg acgataacac
     6961 ctggaccgtt accgttgccg atctgaaaaa tggcactgca cagaacattc gtgccaaatt
     7021 tgtctttatc ggcgcgggcg gtgcggcgct gaagctgtta caggaatcgg ggattccgga
     7081 agcgaaagac tacgccggtt tcccggtggg cggacagttc cttgtttcgg aaaacccgga
     7141 cgtggttaat caccatctgg cgaaggttta cggtaaagca tccgttggcg caccaccgat
     7201 gtcggttccg catatcgata cccgcgttct ggacggtaaa cgcgtagtgc tgtttgggcc
     7261 atttgccacc ttctcaacca aattcctcaa aaacggttca ttgtgggatc tgatgagttc
     7321 caccaccacc tctaacgtga tgccgatgat gcacgtcggg ctggataatt tcgatctggt
     7381 gaaatacttg gtgagtcagg tgatgttgag tgaagaggat cgttttgaag cgttgaaaga
     7441 gtactatccg caggcgaaaa aagaggactg gcgtttgtgg caagcggggc agcgtgtgca
     7501 gattatcaag cgtgatgccg ataaaggtgg tgtactgcgt ctgggtactg aagtggtcag
     7561 tgaccagcaa gggaccattg ccgcgctgct gggggcatcg ccaggggcgt caaccgctgc
     7621 gccgattatg ttggatctgc tggaaaaagt atttggcgat cgcgtttcca gcccgcaatg
     7681 gcaggctacg ttgaaagcga tcgttccatc ttatggacgc aagctgaacg gtgatgtagc
     7741 ggcaacagaa cgcgagttgc agtacaccag cgaagtgctg ggcctgaaat acgacaagcc
     7801 gcaggcagca gatagtacgc cgaaaccgca gttgaaaccg caacccgttc aaaaagaagt
     7861 ggcggatatt gcgttgtaat gatgtgccac atccggcatg gtatgccgga tgtggcgtaa
     7921 ccgccttatc cgacctacgg ggtggagcac ttcacagcgg tgtaggcctg ataagacgcg
     7981 tcagcgtcgc atcaggcaac cggctcgggc gttagatgtc ggatgcggcg taaccgcctt
     8041 atccaaccta cggggtggag cacttcacag cggtgtaggc ctgataagac gcgccagcgt
     8101 cgcatcaggc aaccggcttg ggcgttagat gtcggatgcg gcgtgaccgc cttatccgac
     8161 ctacggggtg acgcacttca cagcagttta gcgaactacc gcgttgtcaa ttttctcttc
     8221 cgccttccag acgcggtact tcacatctac attgtctggc gtatacacca cgatcggcag
     8281 cttgctgttg taacgcagca ttccagcatc gcccagatac gcggtgacaa atttcttctc
     8341 tttcttgcca tccgggcagg ccatcatcgt ggaaaccggc gagctaactt tatcaaagac
     8401 ataataatca tagccccagc cttccagcgt tttgttttcc agcttcccgc cgagacgatg
     8461 caaattgcaa tcgacttcca gcgtctgacc ggttaacagt tctactttca gggtagattc
     8521 atcttcttgc ggggttaact gaatcacctg acgcttcatc cctttttcag cttgtggata
     8581 aggcgcgatt ttttccagtg gctggacgct ttctgccgcc caggcggaag tggtggcgaa
     8641 agcggcaaac aatactgcag gtagaatggt cttcattttt ttgctttcct tcttcattta
     8701 taggttttca ccgatgatgt cgctgaaggt taacatattc atcagattta ttaatttgct
     8761 ttatgaataa cttaaagaac attcagacaa ttttacctgc tcttttagtg ttaaattctg
     8821 atgagggagc aaaccagcag aaatatctct ggtgaattaa tcgctatata aatatgttta
     8881 taaccatttg aaatgtgagc aaaagcccgt ttttgccacg cttcccgcca ctcttttgat
     8941 cctgctacag gttttacccc gatcggggta tgcatctttg acacatcctt taatatctta
     9001 gcggctataa aaatggctta ttaattatgc ggtttatttg gtcgctctca attttcagag
     9061 cgcgttaatg atggaaggtc aatgtgaaga ttgatgcatc ccgtcggggc atactcactg
     9121 gtcgctggcg caaagccagt aacggtatcc gtccgccctg gtccggtgat gaatctcatt
     9181 ttctgaccca ttgcacccgt tgcgacgcct gtattaatgc ttgtgaaaac aacattctgc
     9241 aacgcggcgc gggcggctat ccgagcgtta atttcaaaaa taacgagtgc agcttctgtt
     9301 acgcctgcgc gcaggcttgc cccgaatcgc tattttctcc gcgccacacc agggcctggg
     9361 atttgcagtt caccattggg gacgcgtgcc tggcgtatca gtcagttgaa tgccgccgct
     9421 gtcaggatag ctgtgagcca atggcaatta tctttcgccc gacgctctcc gggatctacc
     9481 agccgcaact taatagccaa ctttgcaacg gatgtggagc atgtgcagcc agctgtccgg
     9541 tatcagccat aactgcggag tatctccatg cacactaact ggcaagtttg cagcctggtc
     9601 gtgcaggcca aaagcgaacg aatttcagac atcagcaccc aactgaacgc ctttcccggc
     9661 tgtgaagttg ctgtcagcga cgcgccgagc ggtcagttga ttgtggtggt ggaagcagaa
     9721 gacagcgaaa cgctgatcca aaccattgag tcagtacgca acgtagaggg cgtgctggcg
     9781 gtgtcgctgg tttatcacca gcaggaagag caaggtgagg aaacaccatg aaactcagtc
     9841 gtcgtagctt tatgaaagct aacgccgttg cggccgctgc ggcggctgcc ggtctcagcg
     9901 tgccgggcgt tgcccgcgcc gttgttggtc agcaggaagc cattaaatgg gataaagcgc
     9961 cgtgccgttt ctgcggtact ggttgcggcg ttctggtcgg aacgcagcag gggcgtgtgg
    10021 tggcctgtca gggcgacccg gacgcaccgg ttaaccgtgg cctgaactgc attaagggct
    10081 atttcctgcc caagatcatg tacggtaaag accgtttgac gcagccgctg ctgcgtatga
    10141 aaaacggtaa atatgacaaa gaaggcgaat ttaccccaat cacctgggat caggccttcg
    10201 atgtgatgga agagaaattc aaaaccgccc tgaaagaaaa agggccggaa tcgatcggta
    10261 tgttcggttc tggtcagtgg actatctggg aaggttatgc cgcgtccaag ctgtttaaag
    10321 cgggcttccg ttcgaacaac atcgacccga acgcgcgtca ctgtatggcg tcggcagtag
    10381 ttggctttat gcgtaccttt ggtatggatg agccgatggg ctgctatgac gacatcgagc
    10441 aggctgacgc gtttgtgctg tggggcgcaa acatggcgga gatgcacccg atcctctggt
    10501 cgcgcatcac taaccgtcgt ctctctaatc agaacgtcac cgtggcggtg ctttctacct
    10561 accagcatcg tagcttcgag ctggcggata acggcatcat ctttacgcca cagtctgacc
    10621 tggtgatcct gaactacatc gccaactata tcattcaaaa caatgcgata aatcaggact
    10681 tcttcagcaa acacgttaac ctgcgcaaag gggcgacgga catcggctac ggtttacgtc
    10741 cgacccatcc gctggaaaaa gcagcgaaga atccaggttc tgacgcctcc gaaccgatga
    10801 gctttgaaga ttacaaagcc ttcgttgccg agtatacgct ggaaaaaacc gctgaaatga
    10861 ccggcgtgcc gaaagaccag ttagaacaac tggcgcagct gtatgccgat ccgaacaaga
    10921 aagtcatctc ctactggacg atgggcttca accagcatac tcgtggcgtg tgggctaaca
    10981 acctggtcta caacctgcac ctgctgaccg gcaaaatttc ccagccgggt tgcggtccgt
    11041 tctccctgac cgggcaacct tctgcgtgtg gtactgcgcg tgaagtgggc acctttgctc
    11101 accgtctgcc tgcggacatg gtggtgacca acgagaaaca ccgtgatatc tgcgagaaga
    11161 agtggaatat cccgagcggc accattccgg cgaaaatcgg tctgcacgcg gtggcacaag
    11221 accgtgcgct gaaagacggc aaactgaatg tttactggac catgtgtacc aacaacatgc
    11281 aggccgggcc gaacatcaat gaagagcgta tgccgggctg gcgcgatccg cgcaacttca
    11341 tcatcgtctc cgatccgtat ccgacagtca gtgcgctggc cgccgacttg atcctgccga
    11401 ccgcaatgtg ggtagagaaa gagggtgctt acggtaacgc cgaacgccgt actcagttct
    11461 ggcgtcagca ggtacaggca ccgggcgaag cgaaatcgga tctctggcag ttagtccagt
    11521 tctcccgccg cttcaaaact gaagaagtgt ggccggaaga gctgctagcg aagaaaccgg
    11581 aactgcgtgg caaaacgctg tacgaagtgc tttatgccac accggaagtg agcaaattcc
    11641 cggtatccga actggcggaa gatcagctga acgatgaatc ccgcgagctg ggcttctatc
    11701 tgcaaaaagg gctgttcgaa gagtacgcat ggtttggtcg cggtcacggt cacgatctcg
    11761 cgccgttcga tgactaccac aaagcgcgcg gtctgcgctg gccggtggtg aacggtaaag
    11821 aaacgcagtg gcgttacagc gaaggtaacg acccgtacgt gaaagcgggc gaaggctata
    11881 agttctacgg taaaccggat ggtaaagcgg tgatcttcgc gctgccgttc gaaccggcgg
    11941 cggaagcacc ggatgaagag tacgacctgt ggctctctac cggacgcgtt ctggagcact
    12001 ggcacactgg cagtatgact cgccgtgtgc cggaactgca ccgcgccttc ccggaagcgg
    12061 tcttgtttat tcacccgctg gatgcgaaag cgcgcgatct gcgccgtggc gacaaagtga
    12121 aagtggtttc tcgccgtggc gaagtgatct cgattgttga aacgcgcggt cgtaaccgtc
    12181 cgccacaggg cctggtgtac atgccgttct tcgacgccgc acagctggtt aacaaactaa
    12241 cgctggacgc gaccgatccg ctctcgaaag agacggactt caagaagtgc gcagtcaaac
    12301 tggagaaggt gtaagccatt atgtcccggt cagcgaaacc tcaaaatggt cgccgccgct
    12361 ttctgcgcga tgttgttcgc acagcaggcg ggctggctgc cgtgggtgtg gcgctggggt
    12421 tacaacagca aaccgcacgc gcatctggcg tgcggttgcg cccgcccgga gccataaacg
    12481 agaacgcctt tgccagtgcc tgtgtgcgtt gcggtcagtg tgttcaggct tgcccttacg
    12541 acaccttaaa actggcgacg ctggcttctg gcctgtcggc gggcacgcca tattttgtcg
    12601 cacgggatat tccttgcgaa atgtgtgagg acattccgtg cgccaaagtg tgcccgagtg
    12661 gtgcgctgga tcgtgagatc gaatcgatcg acgacgcgcg gatggggctg gcggtgctgg
    12721 tggaccagga aaactgtctc aactttcagg ggctgcgctg cgatgtttgt tatcgcgaat
    12781 gcccgaaaat tgatgaggcc atcaccctgg agctggagcg caacacgcgt accggtaagc
    12841 acgcccgctt tctgccgacg gttcacagcg acgcctgtac cggctgcggt aagtgcgaaa
    12901 aagtgtgcgt gctggaacaa ccggcaatca aggtgttacc gctgtcactg gcgaaagggg
    12961 agttaggtca ccattaccgc ttcggctggc tggaggggaa caatggcaaa tcgtaaacgt
    13021 gacgccgggc gcgaggcgct ggtgaaaaaa ggctggtggc gcagtcaccg ttggctggtg
    13081 ttacgtcgcc tttgtcagtt cttcgtgctg gggatgtttt tgagcggtcc gtggtttggt
    13141 gtgtggatct tacacggcaa ctacagctgt agcctgttat tcgacaccgt tccgctgacc
    13201 gatccgctga tgacgctgca aagtcttgcc agtggtcatc tgcctgccac ggtggcgttg
    13261 accggtgcgg tgataatcac tgtgctctac gccctggcag gaaagcgatt attttgcagc
    13321 tgggtctgcc cgctgaaccc gattaccgac ttagcgaact ggttacgcag gcgctttgac
    13381 ctcaatcagt ctgcgacgat ccctcgccac attcgctacg tgctgttggt ggtcattctg
    13441 ctgggatcag ccttaaccgg cacgctcatc tgggaatgga tcaaccccgt ttccctgatg
    13501 gggcgtagcc tggtaatggg cttcggcagc ggcgcgctgc ttattctcgc gctgttttta
    13561 tttgatctac tggtcgttga acacggctgg tgcgggcaca tttgtccggt tggcgcgctg
    13621 tatggcgtgc tgggtagcaa aggtgtgatt accgttgcgg ccagcgatcg gcagaaatgt
    13681 aaccgctgta tggattgttt tcatgtttgc ccggaaccgc atgtgctacg tgccccggtg
    13741 ctggatgaac aaagcccggt gcaggtcacc agccgcgatt gcatgacgtg cggtcgctgc
    13801 gtggatgtct gttctgagga tgtatttaca ataactacac gatggagttc gggagcgaaa
    13861 tcatgaaaag ccatgacctg aagaaagcgc tgtgtcaatg gacggcgatg ctggccctgg
    13921 tggtaagcgg cgcggtttgg gcggctaatg gcgtcgattt tagccagtcg ccggaagtat
    13981 ccgggacgca ggaaggggcc attcggatgc caaaagagca ggatcggatg ccgctgaact
    14041 atgtgaatca gccgccaatg atcccgcata gcgttgaagg ttatcaggta acgaccaata
    14101 ccaaccgctg cttgcagtgc cacggtgtcg aaagctatcg caccactggc gcgccgcgta
    14161 tcagtcctac tcactttatg gacagcgacg gcaaagtggg cgcggaagtg gctccgcgtc
    14221 gttatttctg tctgcaatgt cacgtaccgc aggccgatac cgcgccaatc gtggggaata
    14281 cctttacccc atcaaaaggt tacgggaaat aagaggttat tatgggaaat tctgaccgta
    14341 agcctggtct gattaagcgc ctgtggaaat ggtggcgtac ccccagccgt ctggcgctgg
    14401 gaacgctgct gttgatcggt tttgtcggcg gcatcgtctt ctggggcggc tttaacaccg
    14461 gaatggaaaa agccaatacc gaagagttct gcattagctg ccacgaaatg cgtaacacgg
    14521 tgtatcagga atacatggat tccgtgcact acaacaaccg tagcggcgtc cgtgcgacct
    14581 gtccggattg tcacgttccg cacgagtttg tgccgaagat gatacgcaag ctcaaagcaa
    14641 gtaaagagct gtatggtaaa atttttggcg ttattgacac gccgcagaaa tttgaagctc
    14701 atcgtctgac aatggcacag aatgagtggc ggcgcatgaa ggacaataac tcgcaggagt
    14761 gccgtaactg tcacaacttc gagtatatgg atacaaccgc ccagaaatcg gttgccgcga
    14821 agatgcatga ccaggcggtg aaagatgggc aaacctgtat tgattgccat aaagggatag
    14881 cgcacaagct gcccgatatg cgtgaagtcg agccaggttt ttaacagggt tattgcgtgg
    14941 gtatgcttga agccagagag ttactttgtg agcgggatga acgaacctta tttagtggct
    15001 tgtcatttac gctgaacgca ggagagtggg tacaaatcac cggtagcaac ggcgcgggga
    15061 agacaacgct tctccgtttg ctgacggggt tgtctcgccc tgacgcaggc gaggttctct
    15121 ggcaagggca gcccttgcat caggtacgcg acagctacca tcaaaacctg ttatggatag
    15181 gccatcagcc ggggatcaaa acccggctga cggcgttaga aaatctgcac ttttatcatc
    15241 gcgatggcga taccgcacaa tgtctggaag ccctggcgca ggccgggctt gccggattcg
    15301 acgatattcc ggtaaatcaa ctttctgccg ggcaacaacg ccgcgtcgct ttagcgcgtc
    15361 tgtggctgac ccgtgccacg ttatggatcc tcgacgagcc ttttaccgcg attgacgtta
    15421 acggcgtcga tcgtctgacc cagcgtatgg cgcagcatac ggagcagggg gggattgtga
    15481 ttctgactac ccaccagccg ctcaacgttg ctgaaagtaa aattcgccgc atttcactga
    15541 cgcaaacgag ggccgcatga tgttctggcg cattttccgt cttgagctgc gtgtagcgtt
    15601 tcgccatagc gccgaaatcg ccaacccgct gtggttcttc ctgattgtaa ttaccctttt
    15661 tccgctcagt atcggtccgg agccgcaact gctggcgcgt attgcaccgg gcattatctg
    15721 ggttgctgcg ctgctttcat ccttgctggc gctggaacga ctgttccgtg acgatttgca
    15781 ggacggcagt cttgaacaat tgatgttgtt gccgttaccc ttgcccgccg ttgtgctggc
    15841 gaaggtgatg gcgcactgga tggtaaccgg tctgccgtta ctcatccttt cgccactggt
    15901 agcaatgcta ctgggaatgg atgtttatgg ctggcaagtg atggcgctga cgctgctgct
    15961 gggaacgcct acgcttggct ttctcggtgc accgggtgtg gcgttgacag tgggacttaa
    16021 gcgcggtggt gtgctgctca gcatactggt gttaccgctg actatcccat tactcatctt
    16081 tgccaccgcc gcgatggacg cggcttccat gcatttgccc gttgacgggt atctggcaat
    16141 tttaggcgcg ttgctggcag gcaccgcgac attaagtcct tttgcgacgg cggcagcgtt
    16201 acgaatcagc attcaataac gctggattac tgatttttgt tcgagtctgg tatcgaaact
    16261 atgtggaaaa cactgcatca actggcgatc ccaccacggc tgtatcaaat ctgtggctgg
    16321 tttataccgt ggctggcaat tgccagtgtg gtcgtgctta ccgtcggctg gatctgggga
    16381 ttcggctttg ctccggctga ttatcagcag ggaaatagct accgcattat ctacctgcat
    16441 gtgcctgcgg cgatctggtc gatgggcatt tatgcatcaa tggcagtggc agcgtttatt
    16501 ggccttgtct ggcagatgaa aatggccaac ctggcggtgg cggcgatggc ccccattggt
    16561 gccgtgttta cctttattgc cctggttacc ggctctgcat ggggaaaacc gatgtggggc
    16621 acctggtggg tatgggatgc acgtctgact tctgaactgg tgctgctgtt tttgtatgtg
    16681 ggtgtgattg ccctgtggca cgccttcgac gaccgccgtc tggcgggccg tgcggcaggt
    16741 atcctggtgc tgattggcgt ggtgaatctg ccgattattc attactccgt ggagtggtgg
    16801 aacaccctgc atcagggatc aacgcggatg cagcaaagta tcgatccggc gatgcgttcg
    16861 ccgctgcgct ggtcgatttt tggcttcctg ctcctgtctg ccacgctgac gctgatgcgg
    16921 atgcgtaatt tgattttgct gatggaaaaa cgccgtccgt gggtgagtga actgatactg
    16981 aaaagaggcc gtaaatgacc cctgcatttg cttcctggaa tgaatttttc gcaatgggcg
    17041 gttacgcctt ttttgtctgg ctggcggtgg tgatgaccgt tattccgctg gtggttttgg
    17101 tcgtgcactc ggtgatgcaa catcgcgcaa ttctgcgtgg tgtggcgcaa cagcgggcgc
    17161 gtgaggcgcg tttacgtgct gcgcaacagc aggaggctgc atgaatattc gccgtaaaaa
    17221 ccgcttgtgg attgcctgtg ccgtgttggc agggctggcg ctgactatcg gtctggtgct
    17281 atatgcgctg cgctcgaata tcgatctctt ttatacgccg ggggaaattc tctacggcaa
    17341 gcgtgaaact cagcaaatgc cggaagtcgg tcagcgtctg cgcgttggcg ggatggtgat
    17401 gctgggtagt gtgcagcgcg atcccaattc gctgaaagtg accttcacta tttacgatgc
    17461 tgaaggctca gtggatgtct cttacgaagg cattttgccg gatctgttcc gtgaagggca
    17521 gggcgttgtg gtgcagggcg agctggaaaa aggcaatcat atcctcgcga aagaagtgct
    17581 ggcgaaacac gacgaaaact acacgccgcc agaagttgag aaagcgatgg aagctaacca
    17641 ccgtcgcccg gcgagtgttt ataaggaccc agcatcatga tgccggaaat tggaaacggg
    17701 ctgctgtgtc tggcgttagg gattgcgctg ctgctgtccg tgtatccgct atggggcgtg
    17761 gcgcgcggag atgcgcgcat gatcgcgtct tcccgcttgt ttgcctggct gctgtttatg
    17821 tctgtggctg gcgcatttct ggtgctggtc aatgctttcg tggtcaacga ctttaccgtc
    17881 acctatgttg ccagctactc caatacccag cttccggtgt ggtatcgcgt ggcggctacc
    17941 tggggcgcgc atgaaggctc gctactgctg tgggtgctgc tgatgagcgg ctggaccttt
    18001 gcggtggcga tttttagtca gcgtattccg ctggatattg tggcccgcgt actggcgata
    18061 atggggatgg tcagcgtcgg ctttttgctg ttcattctct ttacctctaa cccattctca
    18121 cgcacgctgc cgaacttccc gattgaaggg cgcgatctta acccgctgtt acaggacccg
    18181 gggctgatct tccatccgcc tctgctttat atggggtacg tgggtttctc ggtggcgttt
    18241 gcttttgcca ttgcttcttt gctgagcggg cgtctggaca gcacttatgc gcgttttact
    18301 cgcccgtgga cgctggcggc gtggattttc ctgacgctcg gcattgtgct cggttccgca
    18361 tgggcctatt acgaactcgg ctgggggggc tggtggttct gggatccggt agaaaacgcc
    18421 tcgtttatgc cgtggctggt ggggactgcg ctgatgcact cactggcggt cactgaacaa
    18481 cgcgccagct tcaaagcgtg gacattactg ctggcaatca gtgccttctc gttgtgtctg
    18541 ttggggactt tcctcgtgcg ttccggcgtg ctggtatcgg tacacgcgtt tgcgtctgat
    18601 ccggcgcgcg gtatgtttat cctcgccttt atggtgctgg tgattggagg ttcgctgctg
    18661 ctgtttgccg cgcgtggaca caaagttcgc tcacgcgtaa acaatgcgct gtggtcgcgg
    18721 gaatctctgc tgttagcgaa caacgttttg ctggtcgccg cgatgctggt ggtgttgctg
    18781 gggacgctgc tgccgctggt gcacaagcaa ctgggactgg gcagtatttc gattggcgaa
    18841 ccgttcttca acaccatgtt tacctggctg atggtgccgt ttgcgctact gctgggtgtc
    18901 ggtcctctgg tgcgctgggg gcgcgatcgc ccacgtaaaa tccgcaattt attgattatc
    18961 gccttcatct caacgctggt gctgtctctg ctgttgccgt ggctgtttga gagcaaagtt
    19021 gtggcgatga cggtgctcgg cctggcaatg gcctgctgga ttgcggtgct ggcaattgcg
    19081 gaagctgcgc tgcgtatttc acgcggcacg aaaaccacct tcagttattg ggggatggtg
    19141 gctgctcacc tggggctggc agtgacaatt gttggcattg cttttagcca gaactatagc
    19201 gttgagcgtg atgtgcgcat gaagtccggc gatagcgtcg atattcatga atatcgcttc
    19261 accttccgtg atgtcaaaga ggtgactggc ccgaactggc gtggcggtgt ggcgactatc
    19321 ggcgtaacgc gagagggtaa accggaaaca gtgctgtatg cggaaaaacg ttattacaac
    19381 actgccgggt cgatgatgac cgaagcggcg attgacggtg gcatcacgcg tgacctgtac
    19441 gccgcgctcg gtgaagagct ggaaaacggc gcgtgggctg tgcgtcttta ctacaaacca
    19501 tttgttcgct ggatttgggc gggcgggctg atgatggcgt tgggcggact gctgtgtctg
    19561 tttgatcctc gctatcgtaa gcgcgtgagt ccgcaaaaaa ctgcgccgga ggctgtatga
    19621 agcgcaaagt attgttaatt ccgttgatta tcttcctggc gattgccgcg gcgctgctgt
    19681 ggcagctggc gcgtaatgcc gaaggggatg atccgaccaa tctggaatcg gcgctcattg
    19741 gcaagccagt gccgaagttt cgcctcgaat cactggacaa tccggggcag ttttatcagg
    19801 cggatgtgct gactcagggc aaaccagtgc tgcttaacgt ctgggcgacc tggtgtccga
    19861 cctgccgtgc ggaacatcaa tatctgaatc agctttctgc gcagggcatc cgcgtagtcg
    19921 gcatgaacta taaagacgat cgccagaagg caatcagctg gctgaaagag ctgggcaatc
    19981 cttacgcgct aagcctgttt gatggcgatg gcatgttagg gctggatctc ggtgtctatg
    20041 gcgcgccaga aacgttcctt attgacggca acggcatcat tcgctatcgc catgcgggcg
    20101 atctcaatcc tcgcgtctgg gaagaagaga tcaagccgct gtgggagaaa tacagtaagg
    20161 aggccgcaca atgaggtttt tattgggcat gctgatgctg atgatctccg gctcagcgct
    20221 ggcgaccatc gacgtgttgc agtttaaaga tgaagcacag gaacaacagt tccgtcagct
    20281 cactgaagaa ctgcgctgcc cgaaatgcca gaacaacagc attgccgatt ccaactcgat
    20341 gattgccacc gacctgcgcc agaaagtgta tgaactgatg caggaaggta aaagtaaaaa
    20401 agagattgtc gattatatgg tggcgcgtta cggcaacttc gtcacttacg atccgccgtt
    20461 aacgccgctg accgtgctgc tgtgggtgct gccagtagta gctattggca ttggcggttg
    20521 ggtcatatac gcccgctcgc ggcgtcgggt acgcgtggtg ccggaagcgt ttcctgaaca
    20581 aagcgtgccg gaaggtaagc gtgccggata tgtcgcttat ttgccgggca ttgtggtggc
    20641 gttaattgtg gctggcgtca gctactacca gaccggaaat tatcaacagg tgaaaatctg
    20701 gcagcaggcc acggcacagg ctccggcgtt gctggacagg gcgctggatc cgaaagctga
    20761 tccgctcaac gaagaagaga tgtcgcgcct tgcgctgggg atgcgtactc aactgcaaaa
    20821 aaatccggga gatatagacg gctggattat gttgggccgc gttggcatgg cgctgggtaa
    20881 cgccagtatc gccaccgatg catacgctac tgcgtatcgc ctcgatccga aaaacagtga
    20941 tgccgcactg ggttatgctg aagcgttgac acgttcatct gatcccaacg acaaccgcct
    21001 cggcggcgaa ctgctacgtc agctggtgag aacggaccat agcaatatcc gtgtgctaag
    21061 catgtatgcg tttaatgcct ttgagcagca gcgatttggc gaagccgttg ccgcgtggga
    21121 gatgatgttg aaactcttac ctgccaacga tactcgccgt gcggtgattg aacgtagtat
    21181 cgcgcaggcg atgcaacatt tgtcgccgca ggagagtaaa taaaaagagg cccgatggtg
    21241 atgccatcgg gctattttta ttgtgccccg cgttgttgca ggaacagaat ggtggccgcc
    21301 acgcgtgagc ggacattgag tttacgcagc agatttcgaa tatgaacttt tactgtctgc
    21361 tcggaaatat tcaacacaga ggcaatctgt ttatttgaca gcccctgtgc cagctcgtgc
    21421 agaacatcca gctcgcgctc cgtcagcacg ctgaagggat cttcttccgc gccaaacatt
    21481 tcacgttcac gtaagtactg attgacgcgt tcgctaaaga ctttgctgcc tttcgctccg
    21541 gcacgaatcg cttccagcaa cacttccggg tcgctgtctt tcagcagata accgtctgcg
    21601 cctgcgtcta tcagcgcaaa gacatcgctg gaggcatcgg atacggtcag gataataatt
    21661 tgcgcggtaa cgccatccct gcgcaaggca ttgagggtat ccaggccact catacctttc
    21721 atattgagat ccagcaagat cacgtcgata tccagtctat tcgccagatc gatcgcgctc
    21781 gcgccgtcgc ccgcttcggc gaccacttca aagccaggat caagctccag taactgacga
    21841 acaccgcgtc gcataagtgg atgatcatcc acaatcatca cctgaaaagg cgttgcttca
    21901 ggcatagtag tctcctgagg ttttattaga atcattatgt ttgagcgtag cgttggtaac
    21961 gctaccaggc gcgtgaatgt taccccattt tatgtatgga catgtagtga aaactacaac
    22021 cttgccgttc gctttggcat cattttcatt ataaagacgg aattttgcat cagcttctca
    22081 tcaagaaaaa caactaaaaa atcaatcaca atacttgtgc atttctttaa acagttttta
    22141 aaaactgact ccgggtatgg agctatgggt attttctgta cccaatgctt ttaacagcaa
    22201 ttaatttcat aggatgaaag ctcaatgcat caatctggtt ctgtttctct ttgtcgttcc
    22261 gcaatatctg ttctggtggc tacagcgtta tattcaccca tagcattggc atcaacagtt
    22321 gagtatggtg agacagttga tggtgttggc ctggaaaaag atatccagct ggtttatggg
    22381 accgccaata atacgaaaat caatcctggc ggagaacagc atataaaaga atttggtgta
    22441 agtagtaata ctgaaattaa cggcgggtat cagtacattg aaatgaatgg cactgcagaa
    22501 tactcagtat taaatgatgg ttatcaaatt gttcaaatgg gtggcgcggt aaaccagact
    22561 acgctcaata atggagtgct acaggtttat gg
//
LOCUS       DAZPLI010000056        22573 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZPLI010000056 DAZPLI010000000
VERSION     DAZPLI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22573
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.056"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            38..580
                     /locus_tag="ACHUU3_002088"
     CDS             38..580
                     /locus_tag="ACHUU3_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2082156.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            663..1364
                     /locus_tag="ACHUU3_002089"
     CDS             663..1364
                     /locus_tag="ACHUU3_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR2082157.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1389..3988
                     /locus_tag="ACHUU3_002090"
                     /pseudo
     CDS             1389..3988
                     /locus_tag="ACHUU3_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3979..5049
                     /locus_tag="ACHUU3_002091"
     CDS             3979..5049
                     /locus_tag="ACHUU3_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2082158.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5061..5603
                     /locus_tag="ACHUU3_002092"
     CDS             5061..5603
                     /locus_tag="ACHUU3_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2082159.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5611..6126
                     /locus_tag="ACHUU3_002093"
     CDS             5611..6126
                     /locus_tag="ACHUU3_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2082160.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6092..6829
                     /locus_tag="ACHUU3_002094"
     CDS             6092..6829
                     /locus_tag="ACHUU3_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR2082161.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6940..7950
                     /gene="pyrD"
                     /locus_tag="ACHUU3_002095"
     CDS             6940..7950
                     /gene="pyrD"
                     /locus_tag="ACHUU3_002095"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR2082162.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8124..8666
                     /gene="zapC"
                     /locus_tag="ACHUU3_002096"
     CDS             8124..8666
                     /gene="zapC"
                     /locus_tag="ACHUU3_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR2082163.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="ACHUU3_002097"
     CDS             complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="ACHUU3_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR2082164.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10016..12124
                     /gene="rlmKL"
                     /locus_tag="ACHUU3_002098"
     CDS             10016..12124
                     /gene="rlmKL"
                     /locus_tag="ACHUU3_002098"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR2082165.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNLGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12136..14043
                     /locus_tag="ACHUU3_002099"
     CDS             12136..14043
                     /locus_tag="ACHUU3_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR2082166.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14173..15426
                     /gene="pqiA"
                     /locus_tag="ACHUU3_002100"
     CDS             14173..15426
                     /gene="pqiA"
                     /locus_tag="ACHUU3_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR2082167.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15431..17071
                     /gene="pqiB"
                     /locus_tag="ACHUU3_002101"
     CDS             15431..17071
                     /gene="pqiB"
                     /locus_tag="ACHUU3_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR2082168.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17068..17631
                     /gene="pqiC"
                     /locus_tag="ACHUU3_002102"
     CDS             17068..17631
                     /gene="pqiC"
                     /locus_tag="ACHUU3_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR2082169.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17887..18054
                     /gene="rmf"
                     /locus_tag="ACHUU3_002103"
     CDS             17887..18054
                     /gene="rmf"
                     /locus_tag="ACHUU3_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR2082170.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="ACHUU3_002104"
     CDS             complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="ACHUU3_002104"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR2082171.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18711..20471)
                     /locus_tag="ACHUU3_002105"
     CDS             complement(18711..20471)
                     /locus_tag="ACHUU3_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR2082172.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20657..21109
                     /gene="matP"
                     /locus_tag="ACHUU3_002106"
     CDS             20657..21109
                     /gene="matP"
                     /locus_tag="ACHUU3_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR2082173.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="ACHUU3_002107"
     CDS             complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="ACHUU3_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR2082174.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
BASE COUNT         5779 a         5293 c         5925 g         5576 t
ORIGIN      
        1 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
       61 gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata atgctatcac
      121 ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      181 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      241 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      301 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      361 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      421 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggattctgc
      481 tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
      541 tgtgaatgcg gtaacaaatt tccatattaa ctattattaa tagaactcat taattgtttt
      601 attaattagt acccctctag tgttctggag gggatattca tattttttaa gagtgactat
      661 ttatgaaaac ttgcataaca aagggaattg tgaccgtaag tttaacggca atattactca
      721 gctgctcatc cgcatgggcg gcaggtaaag gcgggattgg acttgcagca acacgtcttg
      781 tttattcaga aggtgaagag caaatttcac tgggtgtacg taacaccagt ccggatgttc
      841 cttatcttat tcagtcatgg gtgatgaccc cagataataa aaaatcagca gattttatta
      901 ttacaccacc gctatttgtg ctgaatccgg ctaatgagaa tctgttacgc attatgtaca
      961 ttggtgcgcc gttggcgaaa gacagagaaa cccttttctt cactagcgta cgggcagtcc
     1021 cttcaacaac gaagcgggaa gagggaaata ccctgaagat tgccacacaa agcgtcatca
     1081 aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg gcgaaactgc
     1141 gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat ttcattaccc
     1201 tgacagacct gaaaataggg ggaaaggtag ttaaaaatca aatgatttcc ccctttgata
     1261 aataccaatt ctctctgcca aagggggcca aaaatagcag cgtaacgtat cgaaccatca
     1321 atgattacgg ggcggaaacg ccacaactca actgtaaatc gtaagccgtc ttcagttaag
     1381 agagcgagat gtatagaact catcgacaac acagcctgtt aagctctggt ggagtgccat
     1441 cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa gctgaaacct
     1501 ggttcgatcc tgcttttttc aaagatgatc cctcaatggt ggccgatttg tctcgtttcg
     1561 aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg aatcagacaa
     1621 ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg attgccgcct
     1681 gtttgacgac tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt ccggctttta
     1741 aacaactgga caaacaagcg tgtgtgccat tggcggagat tattccggat gccagcgtaa
     1801 cgtttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc atcaaaagta
     1861 atgcccgtgg ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg ctattactgg
     1921 gatattcgtt tagcggggct aacagtattc atagcagcgc agacagtgat tctggcgaca
     1981 gctattttct gaatttaaac agtggcgtta atttaggccc atggagattg cgcaacaatt
     2041 caacatggag tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc agctatttgc
     2101 agcgggcggt tattccacta aaaggcgaac tgaccgtagg tgatgattat actgcaggcg
     2161 attttttcga tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac aacatgctgc
     2221 cagacagcct gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc aatgcccaga
     2281 taacgattaa gcaaaatggt tacaccattt accaaactta tgtatcgcct ggtgcttttg
     2341 aaattagtga tctctattcc acgtcgtcga gcggtgattt gttagttgaa atcaaagaag
     2401 cggacggcag cgtcaatagc tacagcgtac cgttttccag cgtgccatta ctccagcgtc
     2461 aggggcgaat caaatacgcg gtgacactgg cgaaatacag aaccaatagt aatgaacagc
     2521 aggagagcaa atttgcctag gccacgttgc agtggggcgg accgtgggga acgacatggt
     2581 atggtggtgg acaatatgct gaatattacc gtgccgccat gtttggtctg ggatttaacc
     2641 ttggcgattt cggagcaatt tcgttcgatg cgacccaggc gaagagtacg ctggcagacc
     2701 aaagcgaaca taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc aaccaattgg
     2761 gcactaattt tcaattgatg ggctatcgct attcgacgtc gggtttctac accctttccg
     2821 acaccatgta taaacatatg gatggctacg aatttaatga cggtgatgat gaagatacgc
     2881 cgatgtggtc gcgttattac aatttgtttt acaccaaacg tggcaaactg caggtcaata
     2941 tctcccagca attaggcgag tacggttcgt tttatttaag tggtagccag caaacttact
     3001 ggcataccga tcaacaggat cggctattac agtttggcta caacacgcaa attaaagatc
     3061 tctcgctggg gggttcctgg aactacagta agtcccgtgg tcaacctgat gctgatcagg
     3121 tgtttgcact aaatttttcc ctgccgctca atctgttgct ccccagaagt aatgatagct
     3181 ataccaggaa aaaaattacg cctggatgac ctctaacacc agcatcgata acgaagggca
     3241 cattacacaa aacctgggtt taacggagac actactcgat gacggtaacc tgagctacag
     3301 cgtgcaacag ggatataaca gcgaggggaa aacggctaat ggtagcgcca gcatggacta
     3361 caaaggggcg tttgcagatg cccgagtggg ctacaactac agcgataacg gcagtcaaca
     3421 acaactgaac tacgctcttt caggcagttt agttgcccat tcacagggca ttaccctggg
     3481 gcaatcgctg ggggaaacta acgtgctgat tgcagcacca ggcgcagaga atactcgtgt
     3541 ggcgaacagc accgggctga aaactgactg gcgcggatat accgttgtcc cctatgccac
     3601 ttcttatcgg gaaaatcgaa tcgcacttga tgcggcatcg ttaaaacgta acgtggatct
     3661 tgaaaatgca gtagtcaacg tggttcccac caaaggggcg ttggttctgg cggagttcaa
     3721 tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag cagggtatac cgctgcgttt
     3781 tggcgcgata gcgacgctgg acggcgtaca gactaatagc ggcattattg atgatgatgg
     3841 ctcgctctat atgtccggtt tgcccgctca gggagcgata actgtacgct ggggcgaagc
     3901 tcccgatcaa atttgtcata tcagttacca gcttaccgaa caacaaatta actctgcgat
     3961 tacgcgaatg gatgccatat gcagataatc tttggagaaa aatgcgtgtt attactacga
     4021 ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa ctgctgctta tagcgggcag
     4081 tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt ttggcgttaa aaccctggag
     4141 gaagaagaaa atacggctgg agtagttaaa gacaaatttt atcagtggaa cgaatcgaat
     4201 gattattatg tttcctgtga ttgcgataaa gacaatgtca gaagtggccg atgggcattc
     4261 gccgcggatt caccgttagt ctatttaggc gacaactggt acaaaattaa tgactatctt
     4321 gccgccaaag ttttattgca ggttaaaggc agttctccta ctgcggttcc tttcgaaaac
     4381 gtgggcacag gggcagatac acgatggcat atttgcgatc ccggcggtca acgtttaggt
     4441 ggccaggggg ctagcggtaa tagcggtagc ttttccctga aaatattgca gccgttcgtt
     4501 ggttcggtcg tcattcctcc tatggcgctg gcgcgattat ttgaatgcta caacataccc
     4561 gcaggtgatt cctgcacgac tacaggtaca ccggttttag tgtattacct gtctggtacg
     4621 atcaattcac ttggctcatg ttccgtcaat gccggagaaa caatcgaggt cgatctgggc
     4681 gacgcatttg cggctaactt tcgtgttgta gggcataagc ctcttggggc cagaacggca
     4741 gaacttgcaa ttccagtcag gtgtaacacg ggaaacgcgg ggttagttaa cgtcaacctg
     4801 agtctgacgg caaccacaga ccccagctat ccccaggcga ttaagacgtc acgtcctggc
     4861 gtgggcgtgg tggtgaccga tagccagaac aacattattt cccctgctgg tggaacatta
     4921 ccgctctcta ttcctgatga tgcagacagt atcgcgcgaa tgaatgtcta tccagtcagc
     4981 acgacaggtg taccaccaga aaccgggcga tttgaagcca cggcaacggt gagaataaat
     5041 tttgattaac gcgtgaacgt atgaagaaaa aaacgatatt tcagtgcgtt atcttgttct
     5101 ttagcattct taacatccat gtcgggatgg ctgggcctga acaagttagt atgcatattt
     5161 atgggaatgt ggtcgatcag ggctgtgatg tcgccaccaa aagtgcatta caaaatattc
     5221 atattggtga ttttaatatc agtgattttc aggccgcgaa taccgtaagc actgctgctg
     5281 atttgaatat cgatatcacc ggttgtgccg ctagtattac tggcgcggac gtccttttta
     5341 gcggcgaggc tgacaccctt acgccgacac tgctcaaact aactgacaca ggcggaagcg
     5401 gtggtatggc aacggggatt gccgtgcaaa ttcttgatgc gcaaagtcag caagaaatcc
     5461 cgctcaatca ggtccagcct cttacgccct taaaagccgg ggataacaca ctcaaatatc
     5521 aacttcgtta taagtccaca aaggcgggag caacgggcgg taatgcgacg gcggttctct
     5581 attttgatct ggtttaccag tgaaggagtg atgttgaaac ggataatctg gattctgttc
     5641 ttactgggat taacgggggg ctgtgagcta tttgcccatg atggcacggt caacattagc
     5701 ggatcgtttc gccgtaatac atgcgtgctg gcacaggata gcaagcaaat caatgtgcag
     5761 ttaggcgatg tctcgctgac acgttttttt catgggaatt atggcccgga gaaatctttc
     5821 atcattaatt tgcaggattg cggaacagat gtcagcacgg ttgacgtcac cttttcagga
     5881 acccccgacg gagtgcagag cgaaatgctg tccattgaaa gtggtacgga tgcagccagc
     5941 gggctggcaa ttgcgattct ggatgatgcg aagatattga ttccgcttaa ccaggcgagt
     6001 aaagactaca gcctacatag cggtaaggtg ccattaactt tttatgcgca attgcgacct
     6061 gtcaatagcg atgtgcagtc gggtaaagtg aatgccagcg cgacatttgt acttcattat
     6121 gactaatacg tggaacagat tggcgctctt gattttcgcc gttttatcgc tgctggtggc
     6181 gggtgaattg cgggcaggag tggtggtcgg cggaacgcga tttatctttc cggcagacag
     6241 agaatcgata tctattttac tgactaatac cagtcaggaa tcctggctta ttaacagtaa
     6301 aatcaaccgc ccaacgcgtt gggccggggg ggaagcgtcg acagtgccag caccattact
     6361 ggccgctccg ccacttattc tcctgaagcc cggtactaca ggcacgttgc gcttgctgag
     6421 aacggaaagc gacatcttgc ctgtggatcg cgaaacgcta tttgagttaa gcattgccag
     6481 cgtgccatcc ggcaaagttg aaaatcagag cgtaaaagtg gcgatgcgct cggtatttaa
     6541 actgttctgg cgacccgaag gtttgccggg cgacccgctg gaagcttatc aacaattacg
     6601 ctggacacgg aattcgcagg gtgtacaact cactaaccca acgccttact acattaacct
     6661 gattcaggtg agcgtaaatg gtaaagcgtt aagtaatgcg ggagttgtgc cgcctaaaag
     6721 ccagcgtcag acaagctggt gtcaggcaat cgcaccgtgt catgtcgcct ggcgggcgat
     6781 aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat ctgccctgaa acaggttcgg
     6841 aaaacgtttg cgtttttttt gccgcaggtc aattcccttt tggtccgaac tcgcacataa
     6901 tacgcccccg gtttgcacac cgggaatcca ggagagttca tgtactaccc cttcgttcgt
     6961 aaagcccttt tccagctcga tccagagcgc gctcatgagt ttacttttca gcaattacgc
     7021 cgtattacag gaacgccgtt tgaagcactg gtgcggcaga aagtgcctgc gaaacctgtt
     7081 aactgcatgg gcctgacgtt taaaaatccg cttggtctgg cagccggtct tgataaagac
     7141 ggggagtgca ttgacgcgtt aggcgcgatg ggatttggat cgatcgagat cggtaccgtc
     7201 acgccacgtc cacagccagg taatgacaag ccgcgtctct ttcgtctggt agatgccgaa
     7261 ggtttgatca accgtatggg ctttaataat cttggcgttg ataacctcgt agagaacgta
     7321 aaaaaggccc attatgacgg cgtcctgggt attaacatcg gcaaaaataa agatacgcca
     7381 gtggagcagg gcaaagatga ctatctgatt tgtatggaaa aaatctatgc ctatgcggga
     7441 tatatcgcca tcaatatttc atcgccgaat accccaggat tacgcacgct gcaatatggt
     7501 gaagcgctgg atgatctctt aaccgcgatt aaaaataagc aaaatgattt gcaagcgatg
     7561 caccataaat atgtgccgat cgcagtgaag atcgcgccgg atctttctga agaagaattg
     7621 atccaggttg ccgatagttt agttcgccat aatattgatg gcgttattgc aaccaatacc
     7681 acactcgatc gttctcttgt tcagggaatg aaaaattgcg atcaaaccgg tggcttaagt
     7741 ggtcgtccgc ttcagttaaa aagcaccgaa attattcgcc gcttgtcact ggaattaaac
     7801 ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg ttatcgctgc gcgtgaaaag
     7861 attgctgcgg gtgcctcact ggtgcaaatt tattctggtt ttatttttaa aggtccgccg
     7921 ctgattaaag aaatcgttac ccatatctaa ttatttcttc gaattcctct ttataagata
     7981 atcagggctt tattttcagc cctggttgtt ttatattcat cgctgttgct tatttagaca
     8041 ttttgtactt ttattgttga ggttattaag cgaagcgaca atggattgtg ttgctgcggt
     8101 tttatagggt aggggagagg cagatgcgaa ttaaaccaga cgataactgg cgttggtatt
     8161 acgatgaaga gcacgatcga atgatgctcg atttagccaa tggtatgcta tttcgctcac
     8221 gttttgcgcg caagatgttg actccagatg ccttttctcc cgcaggcttt tgcgttgacg
     8281 acgccgcgct ctatttctct tttgaagaaa agtgccgcga ttttaattta tctaaagaac
     8341 aaaaagccga actggtgctg aatgcactgg tagcaattcg ctatctcaaa ccgcaaatgc
     8401 cgaaaagctg gcattttgtt tcccatggtg aaatgtgggt gcccatgccg ggcgatgcgg
     8461 cctgcgtttg gttgagcgat actcacgagc aggtcaattt gctggtcgtt gaatctggcg
     8521 aaaatgccgc actatgcctg ctggcacaac cctgcgttgt tattgcgggt cgcgcgatgc
     8581 agttgggcga cgccattaaa atcatgaacg acaggctgaa accgcaggtt aatgttgaca
     8641 gcttcagcct cgaacaggca gtctaacgcg ccaacttaag tgcagtcttc ggtacacagc
     8701 tacagcaaag aatggtgcca tcatcgccca ttgctgattt tttcagcggc gtgacttcgc
     8761 cttctaaaag ctgaacacgg caacttccac aaatgcccgc gcggcaagaa taagggatac
     8821 gaattccctg attttctaat tgctccagca acacctgttg gttatttcca cgaaatgcct
     8881 gtccctgcca gtcaatatct acatttgcgt ccggttgttg cgtgatgttg gcggtatcat
     8941 cagcggcagc tgcgccgtaa atttttgccg gagccgttgc cagaatttcc acctcatcgc
     9001 caacgcgaat cacgccgcta ttacgggcaa ttaaattctg accaaaatcg acatcgccgt
     9061 tatcctgggc agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc ggatgttttt
     9121 gccctttttc tgggctgacg gtggtgaaaa tacagcggct acaaggttta accacatcaa
     9181 acaccacatc accaatgcga atcactttcc agcgatcttc ttcccaggct gacgcgccgg
     9241 aaaccaccag attggggcgg aattgctcca tttttacact ggccggacaa cgttgttgga
     9301 gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata gccatcagca aatgacagag
     9361 gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg ccccacccag cgtaattgca
     9421 cttcgcggga gaaaaatcca cttagccatt tgttgatcgc gtctggcgca attcgcgcgg
     9481 taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca gcaaaacgaa
     9541 catatgcgct actgccatct ggtgcggtga gatgcaagcc atcatgcaca ggtgaaggag
     9601 taaaccgtac catctgggga aactggcgag cggtaataaa cgtaccgtca ggttccgtga
     9661 tcataaagat gcgatcgaag gccagaccac tgacatcagc cagagcatgt gtaagaccaa
     9721 tgccgcgcat cgatttaaca ggatgaataa aaagccgggt taatgtcgcc acggtgcggt
     9781 cctcaaatga aaataagccc tcaactttat gacatgacgc gcttattagc tataatgcgc
     9841 aacaattttc ttagcgcctg tcccattagg ctattttatt tgtcagtttg gccctgggtt
     9901 gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc tccgcgctgt ccatgtccag
     9961 actgactgca acaattacgc ctactgcggt aggctcttag agtaaaagtg acgatatgaa
    10021 ttctctgttt gccagtacgg cccgtgggct ggaagagctg ttaaaaactg aactggaaaa
    10081 cctgggggcc gttgaatgcc aggtggttca gggtggggtc catttcaagg gcgacacacg
    10141 gcttgtttac cagagcctga tgtggagccg cctggcctcg cgtattatgt tgccgctggg
    10201 cgagtgtaag gtttacagcg atttagacct ctatctcggt gttcaggcga tcaactggac
    10261 agagatgttt aatcttggcg cgaccttcgc tgtccacttc agtggtttga atgacaccat
    10321 ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg ctttcacgcg
    10381 gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg ttaacgtctg
    10441 gctgcataaa gaaaccgcca gtatcgctct tgatctgagt ggtgatggtt tacatctgcg
    10501 tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa accctggcag ccgctattgt
    10561 gatgcgaacc ggctggcagc caggaacacc gctgctcgat ccgatgtgtg gttccggtac
    10621 gttgctgatt gaagcggcga tgctagcgac cgatcgcgca ccaggcttgc accgtgggcg
    10681 ttggggcttt agcggctggg tgcagcatga tgaagctatc tggcaggaag tgaaagcgga
    10741 agcgcaaact cgtgcccgta aaggcctggc tgagtatagc tctcattttt acggttcgga
    10801 cagcgacgca cgggtgattc aacgtgcgcg cactaacgcc cgtcttgcgg ggattggtga
    10861 actgatcacc tttgaggtga aagatgtcgc gcaactgacc aatccgctgc cgaaagggcc
    10921 gtacggcaca gtgttgagca acccgccata cggggaacgt ctggacagcg aaccggcgct
    10981 gattgcgctg catagcctgc tgggtcggat catgaaaaac cagtttggtg gctggaatct
    11041 ctctttgttt agtgcctcgc cggatctgct aagctgcttg cagctgcgtg cagacaaaca
    11101 gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa aattaccacg ttgccgaaag
    11161 cactccagac agcaaaccgg cgatggtagc ggaagactac gccaaccgcc tgcgtaagaa
    11221 cctcaaaaaa ttcgagaagt gggctcgcca ggaaggaatt gaatgttacc gcctgtatga
    11281 cgccgatcta ccggaatata acgttgccgt tgaccgttat gccgactggg tggtggtgca
    11341 ggagtatgcg ccgccaaaaa ctattgatgc tcacaaagcg cgtcagcgtc tgttcgatat
    11401 tatcgctgca accatttcgg tactggggat tgcgccaaac aaactggtgc tgaaaacccg
    11461 tgaacgccag aagggcaaaa accagtacca gaaactgggc gagaagggcg agtttcttga
    11521 agttaccgaa tataacgctc acttgtgggt gaacctgacg gattatctcg atactggtct
    11581 gttcctcgat caccgtatcg cccgccgtat gctcggtcag atgagcaaag gcaaagattt
    11641 cctcaacctg ttctcttata ccggtagcgc caccgtgcat gcgggattag gcggcgcgcg
    11701 cagcaccacc accgtggata tgtcgcgtac ttatctggag tgggcagaac gcaacctgcg
    11761 tctgaatggc ctgaccgggc gtgcgcatcg cctgattcag gccgattgcc tggcgtggct
    11821 gcgtgaggca aatgaacagt ttgatctgat ctttatcgat ccgccaacct tctctaactc
    11881 aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat atggcgctga tgaaagattt
    11941 gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg aacaacaaac gtggcttccg
    12001 tatggatctc gacggcctgg caaaactggg actgaaagca caagaaatta cgcaaaaaac
    12061 gctctcccag gatttcgccc gtaaccgcca gatccacaac tgctggctga ttaccgcagc
    12121 ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg catggctgtc gttcagcgac
    12181 gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt ttgtctggtg
    12241 ggtcgcaacg gcgcgggcaa atcgacgtta atgaaaatcc tcaaccgtga acaagggctg
    12301 gatgacggtc gcattattta cgagcaagat ttgattgtag ctcgtctgca acaggatccg
    12361 ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga agaacaagcg
    12421 gaatatctga aacgctatca cgatatttcg cgcctggtga tgaacgaccc gagcgagaaa
    12481 aatctcaacg aactggcgaa ggttcaggaa cagttggatc accacaacct gtggcagctg
    12541 gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaatgt tgcgctgtcg
    12601 tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt gagtaatccg
    12661 cgcgtgctgt tgcttgatga accgacaaac cacctggata ttgaaaccat cgactggctg
    12721 gaagggttcc ttaaaacctt caatgggacg attattttca tctcccacga ccgttcgttt
    12781 atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt gacctatcca
    12841 gggaattacg accagtacct gcttgaaaaa gaagaagccc tgcgcgtgga agaactacaa
    12901 aatgccgagt tcgatcgcaa actggcgcag gaagaggtgt ggatccgcca ggggatcaaa
    12961 gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcaatgcg tcgcgagcgt
    13021 ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc cagccgctcc
    13081 ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg ttgacggtaa gcaactggtg
    13141 aaagattttt cggcccaggt tctacgtggc gacaaaattg ccctgattgg tccgaatggg
    13201 tgcggcaaaa ccacgctgct gaaactgatg ctcggtcagc ttcaagcgga cagcgggcgt
    13261 attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc ggaactggat
    13321 cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat ggttaacggc
    13381 aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa acgggcgatg
    13441 acgccggtac gtgcgctttc tggcggtgag cggaaccgct tgctgctggc gcgtttgttc
    13501 ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga tgtcgaaacg
    13561 ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct ggttagccac
    13621 gatcgtcagt ttgtcgataa caccgttaca gaatgttgga tcttcgaagg cggcggtaaa
    13681 attggtcgtt atgtcggcgg ttatcatgat gcccgtggtc agcaagagca gtatgtggcg
    13741 ctcaaacagc ctgcggtgaa aaaaaccgaa gaagccgccg cggcaaaagc agaaactgta
    13801 aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca gctaccgcaa
    13861 ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcaggtggc ggatgcttcc
    13921 ttcttcagtc agccacatga gcagacacaa aaagtgctcg ccgatatggc tgctgcagag
    13981 caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa aaatggtggc
    14041 tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt gcctgatgaa
    14101 ctgggtttat caggcatgaa aaccaacact ttgttagtca tctgtaaaat agcgcatcat
    14161 taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg ctcgcagtgt
    14221 gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg tccccggtgt
    14281 ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtccgaccgc ctatgcgttg
    14341 gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat gaacgttgca
    14401 ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc tgaggactac
    14461 gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt ttgtctgata
    14521 accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga gcaactggca
    14581 cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc tggtgtgctg
    14641 gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag ctttctcccc
    14701 tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg tcgctggtta
    14761 tgggacgaca tcgccccgat gccagaactg cgccagccgc taaaaccagg cgtcacgggg
    14821 atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc tgatgaaccc
    14881 gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcct gcagtggaca
    14941 ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt gcccatcatg
    15001 gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt cattctgtta
    15061 tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc tggccagtat tatggtgcca
    15121 acgttaaaga tgatcgccat cgcgtggctg tgttgggatg ccaaagggca tggcaagcgc
    15181 gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg ctggtcgatg
    15241 attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg tttaatgagt
    15301 atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac aatgttttct
    15361 gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga gcatgaggag
    15421 tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga agaactggtc
    15481 tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc ttttttatca
    15541 ttacagccat cagggactgg aagtgaccct gatcaccgcg aatgcggaag gaattgaagg
    15601 tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa gcgccacact
    15661 ggctgatgat ttgacgcacg ttgaaatcaa agtgcggctg aattctggta tggaaaaatt
    15721 gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg aagggattag
    15781 cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg cgaaaggcag
    15841 caaaatggat aaatacgatt tgctggactc gccaccgttg gccccgcctg atgcgaaagg
    15901 tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag atccggtgct
    15961 gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacgc aaaaacgcaa
    16021 tatcagttat caactgttca tcaatgcacc ttatgaccga ctggtgacca gcaatgttcg
    16081 cttctggaaa gatagtggca ttgcggttga tctgacgtca gcggggatgc gtgtggagat
    16141 gggctcattg acaacgctgc ttagtggcgg tgttagcttt gatgtgccgg aaggtctgga
    16201 tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc agaagagcat
    16261 tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag attcggtacg
    16321 cggtctgcaa ccgggagctc cggtagaatt ccggggtatt cgcctgggta ccgtaagcaa
    16381 agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc gtattccggt
    16441 actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg cggatgttgt
    16501 tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa ccggaaacct
    16561 ggtcactggt gcactgtatg ttgatcttga tttctatcca aatacgcctg caataaccgg
    16621 tattcgtgaa tttaatggtt atcagattat cccgactgtt agcggcggcc tggcgcaaat
    16681 ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga atccgatgat
    16741 tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc tgcaaacgac
    16801 gctggatagc atgaacaaga tcctcgccag ccagtcgatg cagcagttgc cgacggatat
    16861 gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg gctccgcagc
    16921 ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag aactgcaacc
    16981 ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga aggacaaaaa
    17041 agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat tgcagcactg
    17101 tggctggccg gatgcagctc cggcgaaatt aataaaaact attaccagtt acctgtggtg
    17161 cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt agagcaggtc
    17221 actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga tgtgaagtat
    17281 gtgattgcca acaacaactt gtgggccagc ccgttggatc aacaattgcg caacaccctg
    17341 gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc tctgggaagc
    17401 gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga tggcaaggtc
    17461 attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg tccgttccgt
    17521 ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtact ggccagtgtc
    17581 tggagtcagg aagccgcttc tattgcgcaa gagataaagc gtctacctta attataaaga
    17641 tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtt tctaacgtgc
    17701 ggaaaaagtt gttcctcttt acattttttg tacaaccgac atacccgtgt agctcacaaa
    17761 tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt attgcttaac
    17821 tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt atgagggaaa
    17881 cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg tggttatcag
    17941 gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa tcaaaggtca
    18001 caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc ctgattctgt
    18061 ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc tgtctgatta
    18121 taatcagaag gcagaagtat cctggaacag accgactttc aggtcgctgg cggtatagat
    18181 caaacgacca tcaaccagca cttctccatc cgccaggccc ataatcagac gacggttaac
    18241 aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta cctgaccagt
    18301 gaatttcact tcgccaacgc ccagtgcgcg gcctttacct tcgccgccca gccagccgag
    18361 gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca taaccggatc
    18421 gccaataaag tggcatccga agaaccacag atccggattg atatccagtt ctgcttcaac
    18481 ataccctttg tcgaagttac cacccgtttc ggtcattttg accacacggt ccatcatcag
    18541 catattcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgc gaccagaggc
    18601 aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg taagccttat
    18661 tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga tcagttcgga
    18721 ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc ctgttgcgat
    18781 gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc gccatcccac
    18841 accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca gatggtgaat
    18901 ttgccttctt ctaccgcttt cactagttca ctgtgaagac ttaaatggcg aacgttagct
    18961 gtggggataa tgacaccttg tttcccggtt aactcacgtt gctggcaaat agcaaagaag
    19021 ccttcgattt tctcatttaa accaccgacc ggctgggcgc gaccgaactg atcgactgaa
    19081 cctgtgatag cgatactctg attcaccggc acatcggcga gggcgcttat cagggcgcag
    19141 agttcagcca tcgaggcact atctccatca acttcactgt atgactgctc aaatgtcagc
    19201 gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa cgcttgcatg
    19261 atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc gatgtcggtg
    19321 aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc aaaagcgcgt
    19381 ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg ttcgccttcg
    19441 gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc gaggaaacct
    19501 tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa ggtgtcgcca
    19561 tcacacaggg aggcgacctc tttacactgg cggaggatcc actgcgggct aagcggaagt
    19621 gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtaccggcca ggcatccgct
    19681 cccggtgcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca ccactgggtt
    19741 actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat agcctgctct
    19801 gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc taccagaatg
    19861 actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga ctcatcaaac
    19921 gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca cagcagaggt
    19981 tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg atgcaccaga
    20041 ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag ttgctccgct
    20101 tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc tgcactcact
    20161 gcgtgacgta agcggatgga gtggccggaa acctcatagt gaccgcccac cagttggcct
    20221 gcatcgcttt gtagcgtacg cgcggcattg gcaataagat tcagatactc agactcttcc
    20281 ggggccttcg ccagcataaa agaggaggat gctcgcgtat gcagcaactg ctccagcgca
    20341 aattgcagcc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat caaatgtggc
    20401 tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca tgcaagtttc
    20461 gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat accgtaaccg
    20521 gcactacagc acacgtagaa agcaccgaca atactccagg catgggcgtt aaagctcaca
    20581 ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag agttacacgg
    20641 taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg gttggaaatg
    20701 gaagtatctg gtgaagaagc atcgcgaagg ggagttaatc acccgttaca tagaagccag
    20761 tgccgcccag gaagccgtcg atgtgttgct ctcgctggaa aatgaacccg tgctggtaaa
    20821 tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc aaaccattcg
    20881 ggcaagacga aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa aatcgatcga
    20941 tctggaattt attgtctggc aacgtctggc tggtcttgcg cagcgtcgcg gtaaaacgct
    21001 ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat acgcgaataa
    21061 aatgtcttct ctgaagcagg atctgcaggc attgctgggt aaggaataac tgacgaaagt
    21121 cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc taccagacga
    21181 gaacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg atctctacgc
    21241 gacgatccgg agccaggcag tcgatcagtg cagcacgctg tttcacgttg tcacaggtgt
    21301 tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct gccgggatac
    21361 ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga ccctggttgt
    21421 aagcgtcaga accgatgcgg tcggtgtaac ccagaacaac tacggaaccg tctttcggat
    21481 ccaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg
    21541 ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt
    21601 ccggtgccgg agctggagcc ggagcaacta ctggagctgc ttcgccctga ccgaaacggt
    21661 aggaaacccc caggctcagc atgccgttgt ccggacgagt gccgatggtg tgtgcgtcac
    21721 cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg atcgcgtact
    21781 caacaccgcc agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca taaacgttgg
    21841 atttagtgtc tgcacgccat accataccac ccagacgagt gtagatgtcc aggtcgtcag
    21901 tgattgggta acccagttta gcggtcagtt gaacgccctg agctttgtat gcaccgtttt
    21961 caacgctgcc tttgtacggc atacgaccta accagtcgta acccatttca aagccaacat
    22021 acgggttaac ctggtaacca ccaaaagcac cagcgcccag ttggttttca tgggtcgggc
    22081 cattgttgtt gatgaaacca gtgtcatggt actgggacca gcccagttta gcaccagtgt
    22141 accaggtgtt atctttcgga gcggcctgcg ctacggtagc gaaaccagcc agtgccactg
    22201 caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa atacgccatg
    22261 aatatctcca acgagataac acggttaaat ccttcaccgg gggatctgct caattaactc
    22321 taccgatatc ttcggcttat gccgagcacc cctggcgatg taaagtctac aacgtagttg
    22381 aaaacttaca agtgtgaact ccgtcaggca tatgaaaaaa aagtcttgta taaggtatgt
    22441 ttaatctttt ttgtcagcga caatttacag aagaaaatcg cggaaaccgc ttcagacaag
    22501 cctccgcaag gaaaattagt cacgactgaa agcattggct gggcgacaaa aaaagttcca
    22561 ggattaatcc taa
//
LOCUS       DAZPLI010000057        22318 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZPLI010000057 DAZPLI010000000
VERSION     DAZPLI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22318
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.057"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            166..1599
                     /gene="cscA"
                     /locus_tag="ACHUU3_002108"
     CDS             166..1599
                     /gene="cscA"
                     /locus_tag="ACHUU3_002108"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR2082175.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1607..2602)
                     /locus_tag="ACHUU3_002109"
     CDS             complement(1607..2602)
                     /locus_tag="ACHUU3_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR2082176.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2833..3051
                     /locus_tag="ACHUU3_002110"
                     /pseudo
     CDS             2833..3051
                     /locus_tag="ACHUU3_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3069..4397
                     /gene="dsdA"
                     /locus_tag="ACHUU3_002111"
     CDS             3069..4397
                     /gene="dsdA"
                     /locus_tag="ACHUU3_002111"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR2082177.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4505..6043)
                     /gene="emrY"
                     /locus_tag="ACHUU3_002112"
     CDS             complement(4505..6043)
                     /gene="emrY"
                     /locus_tag="ACHUU3_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR2082178.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6043..7206)
                     /gene="emrK"
                     /locus_tag="ACHUU3_002113"
     CDS             complement(6043..7206)
                     /gene="emrK"
                     /locus_tag="ACHUU3_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR2082179.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7622..8236
                     /gene="evgA"
                     /locus_tag="ACHUU3_002114"
     CDS             7622..8236
                     /gene="evgA"
                     /locus_tag="ACHUU3_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR2082180.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8241..11834
                     /gene="evgS"
                     /locus_tag="ACHUU3_002115"
     CDS             8241..11834
                     /gene="evgS"
                     /locus_tag="ACHUU3_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR2082181.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKNN"
     gene            complement(11890..13035)
                     /gene="yfdE"
                     /locus_tag="ACHUU3_002116"
     CDS             complement(11890..13035)
                     /gene="yfdE"
                     /locus_tag="ACHUU3_002116"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR2082182.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13109..14053)
                     /gene="yfdV"
                     /locus_tag="ACHUU3_002117"
     CDS             complement(13109..14053)
                     /gene="yfdV"
                     /locus_tag="ACHUU3_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR2082183.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14123..15817)
                     /gene="oxc"
                     /locus_tag="ACHUU3_002118"
     CDS             complement(14123..15817)
                     /gene="oxc"
                     /locus_tag="ACHUU3_002118"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR2082184.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15871..17121)
                     /gene="frc"
                     /locus_tag="ACHUU3_002119"
     CDS             complement(15871..17121)
                     /gene="frc"
                     /locus_tag="ACHUU3_002119"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR2082185.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17634..18257)
                     /locus_tag="ACHUU3_002120"
     CDS             complement(17634..18257)
                     /locus_tag="ACHUU3_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR2082186.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18554..18829
                     /gene="ypdI"
                     /locus_tag="ACHUU3_002121"
     CDS             18554..18829
                     /gene="ypdI"
                     /locus_tag="ACHUU3_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFR2082187.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18906..19148)
                     /locus_tag="ACHUU3_002122"
     CDS             complement(18906..19148)
                     /locus_tag="ACHUU3_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR2082188.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19501..20421
                     /gene="lpxP"
                     /locus_tag="ACHUU3_002123"
     CDS             19501..20421
                     /gene="lpxP"
                     /locus_tag="ACHUU3_002123"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR2082189.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20777..20848
                     /gene="ypdK"
                     /locus_tag="ACHUU3_002124"
     CDS             20777..20848
                     /gene="ypdK"
                     /locus_tag="ACHUU3_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR2082190.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20913..22151)
                     /gene="alaC"
                     /locus_tag="ACHUU3_002125"
     CDS             complement(20913..22151)
                     /gene="alaC"
                     /locus_tag="ACHUU3_002125"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR2082191.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT         6292 a         5219 c         4859 g         5948 t
ORIGIN      
        1 actttggctg acattttttt cccttaaatt catctgagtt acgcatagtg ataaacctct
       61 ttctcgcaaa atcgtcatgg atttactaaa acatgcatat tcgatcacaa aacgtcatag
      121 ttaacgttaa caattcacca aatttgctta accaggatga ttaaaatgac gcaatctcga
      181 ttgcatgcgg cgcaaaacgc actagcaaaa cttcatgagc accggggtaa cactttctat
      241 ccccattttc acctcgcgcc acctgccgga tggatgaacg atccaaacgg cctgatctgg
      301 tttaacgatc gttatcacgc gttttatcaa catcacccga tgagcgaaca ctggggacca
      361 atgcactggg gacatgccac cagcgacgat atgatccact ggcagcatga gcctattgcg
      421 ctagcgccag gagacgataa tgacaaagac gggtgttttt caggtagtgc tgtcgatgac
      481 aatggtgtcc tctcacttat ctacaccgga cacgtctggc tcgattgtgc aggtaatgac
      541 gatgcaattc gcgaagtaca atgtctggct accagtcggg atggtattca tttcgagaaa
      601 cagggtgtga tcctcactcc accagaagga atcatgcact tccgcgatcc taaagtgtgg
      661 cgtgaagccg acacatggtg gatggtagtc ggggcgaaag atccaggcaa cacggggcag
      721 atcctgcttt atcgcggcag ttcattgcgt gaatggacct tcgatcgcgt actggcccac
      781 gctgatgcgg gtgaaagcta tatgtgggaa tgtccggact ttttcagcct tggcgatcag
      841 cattatctga tgttttcccc gcagggaatg aatgccgagg gatacagtta ccgaaatcgc
      901 tttcaaagtg gcgtaatacc cggaatgtgg tcgccaggac gactttttgc acaatccggg
      961 cattttactg aacttgataa cgggcatgac ttttatgcac cacaaagctt tttagcgaag
     1021 gatggtcggc gtattgttat cggctggatg gatatgtggg aatcgccaat gccctcaaaa
     1081 cgtgaaggat gggcaggctg catgacgctg gcgcgcgagc tatcagagag caatggcaaa
     1141 cttctacaac gcccggttca cgaagctgag tcgttacgcc agcagcatca atctgtctct
     1201 ccccgcacaa tcagcaataa atatgttttg caggaaaacg cgcaagcagt tgagattcag
     1261 ttgcagtggg cgctgaagaa cagtgatgcc gaacattacg gattacagct cggcactgga
     1321 atgcggctgt atattgataa ccaatctgag cgacttgttt tgtggcggta ttacccacac
     1381 gagaatttag acggctaccg tagtattccc ctcccgcagc gtgacacgct cgccctaagg
     1441 atatttatcg atacatcatc cgtggaagta tttattaacg acggggaagc ggtgatgagt
     1501 agtcgaatct atccgcagcc agaagaacgg gaactgtcgc tttatgcctc ccacggagtg
     1561 gctgtgctgc aacatggagc actctggcta ctgggttaac ataatatcag gtggaacaac
     1621 ggatcaacag cggacaaggg atccgcgtca ctcttccccc ttcacgacct tcaataatat
     1681 gcaatgcagc ttcccgcccg ataatgtcat gtggaagctg aattgtggtc agcggcggta
     1741 aaaacagatg cccgacgcca accagattat caaagcccat tacggcgaca tcctgcggga
     1801 tacgtacccc cttcgccagg agaacctgat aagcgacaaa ggctgcacga tcgttaccac
     1861 atatcagaac atcaaaatct ggtttgcccg atttgaagtg ggcattgagt aaacttgcga
     1921 gatcggtgta gtgatcatca cctgttgcca tgtgaaattg tttcacctca gccagatctc
     1981 gtccagcatc acgccaggcc tgctcaaatc cctgccgacg ataccctgtt gccaacgcac
     2041 tttccggtag ccagaagcat aacggttgac gatagcccgc cgcgagcaaa tgctgtgttg
     2101 attcatattg tgcagtgtaa tcatcaggga tataactggg taacgctggg tcatccgcca
     2161 cacagttcgc caatacaata ttttcaccat acagagactc aggcagcgtg atatgtcgca
     2221 gccccattgt agtatagata atgccatccg gacggtgggc aagcagctga cgtgccgcgc
     2281 gggcagcgtc atcttcagaa aaaatattga ttaaaaaact attccagccg aactcgctgg
     2341 cggtttgctc aatggcaagc agaatatcaa cagagaaagg agtggtagcc gtgtcctgcg
     2401 ccagcacggc gagagtcgac ggcttacgtc cttgagcgcg catcttacgg gcggaaagat
     2461 caggaacata attcagggtc tggattgcct gcaatacgcg gtcacgcgtt gcaggacgca
     2521 cagattctgc attatgcatc acccgggaga ctgtcatcat cgacactccc gccaggcgtg
     2581 cgacatcctt taatgaagcc atacccaagc cgtttgccgt aaaacgggca ctgtagcaga
     2641 aacagacgtc actggcgaga tccaacgccc tatcacctga cacagcaata caataaaaaa
     2701 taacaataat tcccggacaa ttgtccccaa ttccgcctct gttctcgcca acgagtaacc
     2761 atataggcaa gtaagtaaaa catgccaact ggcatcgcca acctggagaa gaaaatataa
     2821 atatggatca ttgtgctgcc gctgtatccc gacatcagcc cggaaattat tgcgattgct
     2881 atcggttcag gtgcaattgg ctgcacgatc gttacggact cgcttttctg gctagtgaag
     2941 caatattgcg gcgctacgct caatgaaaca tttaaatact atacgacagc gacatttatc
     3001 gcttcagtcg tcgctctggc gggcacattc ctgctgtcat ttatcatcta agcgcaaaga
     3061 gacgtactat ggaaaacgct aaaatgaact cgctcatcgc ccagtatccg ttggtaaagg
     3121 atctggttgc tcttaaagaa accacctggt ttaatcctgg cacgacctca ttggctgaag
     3181 gtttacctta tgttggcctg accgaacagg atgttcagga cgcccatgcg cgcttatccc
     3241 gttttgcacc ctatctggca aaagcatttg ctgaaactgc tgccactggg gggattattg
     3301 aatcagaact ggttgccatt ccagctatgc aaaaacggct ggaaaaagaa tatcagcaac
     3361 cgatcagcgg gcaactgtta ctgaaaaaag atagccattt gcccatttcc ggctccataa
     3421 aagcacgcgg cgggatttat gaagtcctgg cacacgcaga aaaactggct ctggaagcgg
     3481 ggttgctgac gcttgatgat gactacagca aactgctttc tccggagttt aaacagttct
     3541 ttagccaata cagcattgct gtgggctcaa ccggaaatct ggggttatca atcggcatta
     3601 tgagcgcccg cattggcttt aaggtgacag ttcatatgtc tgctgatgcc cgggcatgga
     3661 aaaaagcgaa actgcgcagc catggcgtta cggtcgtgga atatgagcaa gattatggtg
     3721 ttgccgtcga ggaaggacgt aaagcagcgc agtctgaccc gaactgtttc tttattgatg
     3781 acgaaaattc ccgcacgttg ttccttgggt attccgtcgc tggccagcgt cttaaagcgc
     3841 aatttgccca gcaaggccgt atcgtcgatg ctgataaccc tctgtttgtc tatctgccgt
     3901 gtggtgttgg cggtggtcct ggtggcgtcg cattcgggct taaactggcg tttggcgatc
     3961 atgttcactg cttttttgcc gaaccaacgc actccccttg tatgttgtta ggcgtccata
     4021 caggattaca cgatcagatt tctgttcagg atattggtat cgacaacctt accgcagcgg
     4081 atggccttgc agttggtcgc gcatcaggct ttgtcgggcg ggcaatggag cgtctgctgg
     4141 atggcttcta tacccttagc gatcaaacca tgtatgacat gcttggctgg ctggcgcagg
     4201 aagaaggtat tcgtcttgaa ccttcggcac tggcgggtat ggccggacct cagcgcgtat
     4261 gtgcatcagt aagttaccaa cagatgcacg gtttcagcgc agaacaactg cgtaatacca
     4321 ctcatctggt gtgggcgacg ggaggtggaa tggtgccgga agaagagatg aatcaatatc
     4381 tggcaaaagg ccgttaataa cgtttcaacg cagcatcgca atcctttccc tgggtgagcg
     4441 atgctgccga tggcgcagac ttaagatccc cggtcttacc cgctataacc ccctcctttt
     4501 atcatcaccc aacgcctttc gctgtaaacg gcggtttcgc aaaccaaacc aaaaccgtaa
     4561 gtaagataaa agcaatagcc gccatacgga aaatctcatt tgcagaaata gaaagtgact
     4621 gctgggttat ttcattatta atttcattaa gaactcctga aagcgaacca taatatttat
     4681 ccataatttg tgacgatgaa ttaaacacgg ggttaaattg atcgatggtt gctgtcaact
     4741 gactatggtg taacgattcg cgtcgtcccc acagcgtcat tgtcaacgac gtaccaactg
     4801 atcctgacaa ggtacgaaaa aaattactca tactcgaggc attggcaaat ttattatctg
     4861 gcaagcctga aaacgaaatc gttgttaagg gtaaaaagaa acaggcaacg gcgaatccct
     4921 gaaaaaactg cggcaaaatg atgcctgtaa aatcaatcgt tggcataaat gtcacagaac
     4981 gccagtaata gcaaaccgca tacatcaaaa aactaaatgt cactaacaac cgcatgtcta
     5041 ttttgttgcc ataacgtcct atcaaaggtg aaattaatag tggcatgatg ccgatgggcg
     5101 cataagcaag tccggcccat atcgcattat accccatcgt ttcctggagt aactgcggca
     5161 taaggacgat cgctccagag taaaataaat acgcgcatgt gatactcaca ataccaatgg
     5221 tgaagttacg ggacttaaac aaactgagat caagaatcgg gttctctgag gtcgactccc
     5281 aaatgactaa agagatcaga gaaataactg atactactgt taatattatt attgtactcg
     5341 agttgaacca atccagatcg cgccctttat caagcataat ttgcaagcca ccaacaccga
     5401 gcactaacag ggtcagtcct ggtagattca ttttgaccgg tgaagtctca gtttctcttc
     5461 ctttaagtaa ggttaagcat aatgtcagga cgataatccc catagggaca ttgattaaaa
     5521 atatccaacc ccagctaaag ttatcacaaa tataaccgcc caatatcggc ccacatatcg
     5581 gagcgataat cacggtcatt gaccataatg ccagagcaaa tgttcttttt tctggcggat
     5641 aattccttaa taataaactc tgtgacagtg gaattaacgg ccccgccatt aacccctgaa
     5701 cgactctaaa aaatatcagc acatcaagat tggtcgatag gctacacatt aatgaagaca
     5761 gcgaaaaaaa ggttactgaa agtaaaaata atcttaattc gcctattctt tgtgccaacc
     5821 tgccagtaac agggatcgca atggcatttg ctacaccaaa cgaggtgata acccaggtgc
     5881 cttcgtctgt tgatgctccc agaaagccag atattgtcgg tattgcgacg ttagaaatag
     5941 tggaatccaa catttgcata aatgtcgcta atgacaatgc aatagtgacg caccataacg
     6001 tcccaccggt taatggtgcc ggagttgatt tagtgattgc cattaaagtt gtccattatg
     6061 cgaaataatg ttgctaattt ctttttctat cggactggta tcgataacta aagccttact
     6121 ggtataagcc ggcatggagg tcacggttga agccagctca ggcatctcag caatgtcttc
     6181 gtccttcgta tcaatagttg cagtcatcga taaaccaatg cgcaaggggt gttccatgag
     6241 ttcttttgga tcaagagaaa cttcaaccgg tacacgctga acgattttga tccagttccc
     6301 tgtcgcattt tgtgcaggta ataaggagaa cgcattgccg gttcccatat tgatccctgt
     6361 cacccgacca tgaaacacaa cattttcacc ataaagatcg ctgataatat tgactgattg
     6421 accaatccgt acatccgtga gttgtgtttc tttaaagttg gcattaaccc acatttgacg
     6481 tgccggtacg acagccatta acgattgtcc gggactcact gtttcgccga cctgaacact
     6541 tcgctgggca atatagccag taaccggact ccttatatcc gtacgtttaa gcgccaacca
     6601 ggcttctttg gttgcatccg ccgcttcaac tacttgtggc tgacggttta atggtgtgtt
     6661 cattactaaa gctttattcg ctttataagc ctggatagcg gcattcagtg ccgctttgct
     6721 acttattaac gtatctttgg tatgctccag cgtttctttt gaaataaccc cctgcttcgc
     6781 taaaggcaca cgacggttat aatcttctaa ggattgttga tactgaatac gtgctgaagc
     6841 gacttcggca ctgtattgtt tatcctgtaa gtacagttta ttcgtttgcc gaacaatatt
     6901 tgccagatta tttttagctt tattgagtgc gatagtggca tcagttttat ccagtgaaac
     6961 taaaatgtca ccctgtcgaa cgtagttcgt atctttatga ttaactacag tgacactacc
     7021 tgagacttgt gcagaaattg gatctgcatt ccccgtgaca taggcgtcat ctgtactaat
     7081 catgtcttct aattccattg accaataggc ataggcacct gaaaacgcaa taaataaaac
     7141 tatcaccaat aaagaaaagt atttttttct gttagactgt tttttatttg aattaatctg
     7201 ttccactatt atctctcatt tcgcatagat gaaatttatg aattgaatga aaagagtatt
     7261 cattcttcat atcaaacaca gtgcatgtgt tgcacattat cccaggaacg ttggaggcct
     7321 aatttaaatg aggtgcaaaa aaaacaacag aggactaaac cgtggctttt gcaatacaat
     7381 tcttacgcct gtaggattag tgagaagact tatagtgcta acttgaaact ataaatcatc
     7441 ggtataatcc ctgattttat tgttgacatt tcatttatgc cgactattta tatggtatac
     7501 ttgtcgaatt atcttaaagg aagctcagat tttcttattt ttattgagaa aatgagatga
     7561 cgccttatgt ctgtattact acagggagaa gggagatgct tcattgcaaa gggaataatc
     7621 tatgaacgca ataattattg atgaccatcc tcttgctatc gcagcaattc gtaatttatt
     7681 gatcaaaaac gatattgaaa tcttagcgga gttaactgaa ggcggaagcg ccgttcagcg
     7741 ggtggaaaca cttaagcctg atatcgtcat cattgatgtc gatatccccg gagttaacgg
     7801 tatcctggtg ttagaaacgc tgaggaagcg ccaatatagc ggaattatta ttatcgtctc
     7861 cgctaaaaat gaccattttt acgggaaaca ttgtgctgat gctggcgcta atggtttcgt
     7921 gagtaaaaaa gaaggcatga acaatatcat tgcggctatt gaagctgcaa aaaatggcta
     7981 ctgctatttc cccttctctc tcaaccggtt tgttggaagt ttaacgtccg accagcaaaa
     8041 actcgactcc ttatcgaaac aagaaattag tgtcatgcgg tatattcttg atggcaagga
     8101 taataatgac attgcggaaa aaatgttcat cagcaacaaa actgtcagca cttataaaag
     8161 tcgcctgatg gaaaaattag aatgtaaatc actgatggat ctttacacat tcgcacaacg
     8221 taacaaaatc ggctaaccac atgaagtttt taccctatat ttttcttctc tgttgtggtc
     8281 tttggtcgac cataagtttc gcagacgaag attacatcga atatcgtggc atcagtagta
     8341 acaaccgtgt catacttgat ccagtacgtc tgagcaacaa ggaattacgt tggttagcga
     8401 gcaaaaaaaa tcttgtgatt gcagtacata agtcccaaac ggctacgttg ttgcataccg
     8461 attcgcagca acgagttcgt ggtattaatg ctgattattt aaatctttta aaaagaacgt
     8521 taaatatcaa attaacactc cgggaatacg ccgatcatca aaaagcaatg gacgcgctgg
     8581 aagaaggtga agtcgatata gtgttatcac atttagtagc ttcgccgcct cttaatgatg
     8641 acattgctgc aaccaaacca ctgataatta cctttccggc gctggtaacc acccttcacg
     8701 attcaatgcg accgcttacc tcatcaaaac cagtaaatat tgctcgagta gcaaattatc
     8761 cccctgacga ggtaattcat caatcatttc caaaagcaac aattatctct tttacaaatt
     8821 tatatcaggc attagcatcc gtctcagccg gacagaatga ttactttatt ggtagtaaca
     8881 tcattaccag cagtatgatt tcccgctatt tcactcactc cttaaatgta gtgaaatatt
     8941 ataactcacc gcgtcaatat aattttttct tgaccagaaa agaatctgtc gttcttaatg
     9001 aagtactcaa tcgatttgtt gatgctttga caaatgaagt tcgctatgaa gtatcacaaa
     9061 attggcttga tacaggaaac ctggcctttc tgaacaaacc attagaactc actgaacatg
     9121 aaaaacaatg gattaagcag catcccgatt taaaggtgct ggaaaatcct tactcccctc
     9181 cctattctat gacggatgaa aatggctcgg ttcggggcgt tatgggggat attcttaata
     9241 ttattacttt gcaaacaggt ttaaattttt ctccgatcac cgtttcacac aatatccatg
     9301 ctggaacaca gctgaatccc ggcggatggg atatattacc cggtgctatt tatagtgaag
     9361 atagagaaaa taatgtttta tttgctgaag ccttcataac aacgccttac gtttttgtca
     9421 tgcaaaaagc gcctgacagt gaacaaacat taaaaaaagg aatgaaagtt gccattccat
     9481 attattatga gcttcattcg caattaaaag agatgtatcc ggaggtagag tggataaaag
     9541 tcgataacgc cagcgctgca tttcacaagg tcaaggaagg cgaacttgat gctctggtcg
     9601 cgacacagtt aaattcacgt tacatgatcg accattacta tcctaatgaa ctttatcatt
     9661 ttcttattcc cggcgttccg aatgcatcgc tttcgttcgc ttttcctcgc ggagaaccgg
     9721 aacttaagga tattattaat aaagcactga atgcaatccc cccaagcgaa gttctgcgcc
     9781 tgaccgaaaa atggattaaa atgcccaatg tgaccattga cacatgggac ctctatagcg
     9841 agcaatttta tattgttacg acattatccg ttttattagt tggcagtagc cttttatggg
     9901 gattctacct gttacgctca gttcgtcgtc gtaaagtcat tcagggtgat ttagaaaacc
     9961 aaatatcatt ccgaaaagca ctctcggatt ccttaccgaa tccaacttat gttgtaaact
    10021 ggcaaggtaa tgtcattagt cataatagtg cttttgaaca ttatttcact gcggattact
    10081 acaaaaatgc aatgttacca ttagaaaaca gtgactcccc ctttaaagat gttttttcta
    10141 atgcgcatga agtcacagca gaaacgaaag aaaatcgaac aatatacaca caggtttttg
    10201 aaattgataa tggcatcgag aaaagatgca ttaatcactg gcatacatta tgcaatctgc
    10261 cagccagcga tcatgctgtt tatatttgtg gttggcaaga tattacagag acgcgtgatt
    10321 taattcatgc actcgaagta gagagaaata aagcgatcaa tgcaactgtc gcaaaaagtc
    10381 agtttctggc aacaatgagt cacgaaataa gaacaccaat aagctccatt atgggcttcc
    10441 tggaacttct gtcgggttct ggtcttagca aggagcaacg ggtggaggcg atttcacttg
    10501 cctactccac cggacaatca ctcctcggct taattggtga aatccttgat gtcgacaaaa
    10561 ttgaatcggg taactatcaa cttcaaccac aatgggtcga tatccctact ttagtccaga
    10621 acacttgtca ctctttcggt gcgattgctg caagcaaatc gatcgcatta atttgcagca
    10681 gtacatttcc tgatcattac ctggttaaga tcgaccctca ggcgtttaag caggtcttat
    10741 caaatttact gagcaatgct ctcaaattta ccaccgaggg ggcagtaaaa attacgacct
    10801 ccctggttca cattgatgac aaccacgctg ttatcaaaat gacgattatg gattctggaa
    10861 gtggattatc gcaggaagaa caacaacaac tctttaaacg ctacagccaa acaagtgcag
    10921 gtcgccagca aacaggatct ggcttaggct taatgatctg caaagaatta attaaaaata
    10981 tgcagggcga tttgtcatta gaaagtcatc caggcatagg aacaacattt acgatcacaa
    11041 tcccggtaga aattagccag caagtggcga ctgtcgaggc aaaagcagaa caccccatca
    11101 cactacctga aaagttgagc atattaatcg cggatgatca tccgaccaac aggctattac
    11161 tcaaacgcca gctaaatcta ttaggatatg atgttgatga agccactgat ggtgtgcaag
    11221 cgctacacaa agtcagtatg caacattatg atctgcttat tactgacgtt aatatgccga
    11281 atatggatgg ttttgagttg actcgcaaac tccgtgagca aaattcttcc ttacccatct
    11341 gggggcttac agccaacgca caggctaacg aacgtgaaaa agggttaagt tgcggcatga
    11401 acttatgttt gttcaaaccg ttgaccctgg atgtactgaa aacacattta agtcagttac
    11461 accaggttgc gcatattgca cctcagtatc gccaccttga tatcgaagcc ctgaaaaata
    11521 atacggcgaa cgatctacaa ctgatgcagg agattctcat gactttccag catgaaacgc
    11581 ataaagatct acccgctgcg tttcaagcac tagaagctgg cgataacaga actttccatc
    11641 agtgtattca tcgcatccac ggtgcggcta acatcctgaa tttgcaaaag ttgattaata
    11701 ttagccatca gttagaaata acacctgttt cagatgacag taagcctgaa attcttcagt
    11761 tgctgaactc tgtaaaagaa cacattgcag agctggacca ggagattgct gttttctgtc
    11821 agaaaaataa ctaaatagcg gctcccacaa tgttctaatg tgggagctat ttaccagcac
    11881 atcttacatt tatgatgaga actcctggcg aatttgttcc ccatgctggt cgagcgttgc
    11941 cgctcccggc ataacatgcg ggtccgcgca gccgctgatt tttatcggat tacccggcat
    12001 cattattcct ccggcttcaa tcaacatatt tctcgcctga gtttgtggca aattaatggc
    12061 ctcagccaca cttaataacg gcgcgacggg cacaccaact tcatgtattt tggctaacca
    12121 aacttcagct gcctgcgttt ttaacgttcg ctcaatatat tgtttaagaa tagcctggtt
    12181 ttgtacgcgt aaaatattgc tgctaaatcg gggatcatta accagttccg taagctccag
    12241 tgcctggcat aacgcagaaa aaagcttgtc attaccacaa caaatcgtaa tcggcttatc
    12301 ctgagtatcg aaaacatcaa aaggtgccat gtagggatgg cgatttccca ggcgttgtgg
    12361 tgacttccct gtcgcaatat atgccatcag accatgctcc agaaaactca gcgtggcatc
    12421 aaacatcgct atatcgacat gcgcccctct ctggctcttt tcgcggccat aaagtgcact
    12481 cactattccg ctgaataaat agacaccgcc gcatagatcc gcaagagagg taccaacacg
    12541 cactggcgga gcatcagggt accctgtttc catcattatc ccactcattg cctgaatgat
    12601 ggtatcgtag gcaggagcat cttttagcgg accggtatgt ccgaaacctg acgatgaagc
    12661 atatatgagg cgcgggttga tttcttgtaa cctttcccat gaaaacccca gtttttccat
    12721 tgtacctggg cgaaaattct cagctaatac atcagcttgt ttgagcatat ttataaatat
    12781 acttttatcg tgatcattct ttaaatcaag aaccacactc tctttgccat gattaataaa
    12841 actgtaatag agtgactgtc catccacata gggaccaaat gtgcgggtat catcaccatg
    12901 acctggtggc tcaactttaa ttacccttgc gcccatatta caaagaagtt gagttccgaa
    12961 aggtccatta aggacatgtg tcatatcgat aactaataag ccttcaaacg gccctttgct
    13021 ttcattattt gtcatttgct tatgccttga aaaaagccga aggtgaaaag acacttcggc
    13081 atttatgtaa aatcaataac atgattaatt atgaaaccag tcgactgaca taaatccaca
    13141 agggagccgt gacgacaaag cccagtacgc tcacagccaa tgacgctgta ccagtacggg
    13201 tgtagacatt aaaccggctg gcaataatga ttccggagaa tgccggcggt aatgcgcctg
    13261 ccagtaccat catctgcaaa tgttcgctgt tcaaatgaca tgccatacca acgagaagca
    13321 gcgccagtgg catcagaatc aacttcagga aggtgttata agcaatttcg gcactgaact
    13381 cgaatttatg tgcagccaga gtcaaccccg cagcgaatac cgctacccct gagttagcct
    13441 tcgcaatcag attaaaggtc gggtcccatg ctgccggaat ttttacccca accagcacca
    13501 ggatcgttgc cagaacaggt gcccatacta ctggctcctt tgccgcagaa attaatgcgc
    13561 tcagattact attcttctta ccatccgctc ctgaggaagg attcagcaaa tacagaccaa
    13621 taggaatagt aattgcgtta acaataatag aaataattgc taccactaaa cctgttgata
    13681 cggaatcacc ataaatagga tcgagaactg caaaccccag gaatccaatg gtaggtgaac
    13741 ccgcaattaa tgcacataca gcggcttctg catgggtacg tttaaaaaat ttgtagcaac
    13801 caaaccagga gaaaaagaaa catccgacaa taaccacaag tgatacaagg gtcagacgag
    13861 tgtccgcaaa aatcatttcc ctgtttgccc gggtaataga aacaaataga gccgcaggaa
    13921 gagcgtagtt taataccagt ttattaaatg cgcgggcttg atcttctgaa aaagtttctc
    13981 gtctgccgct aaaataaccc aacagcatga ttacgataat aggcaataaa tcgccaataa
    14041 aaaatgttag cataatagta tttccgataa gggtcccctc accatcgcat aatgagttgg
    14101 caagaggaga cgatgtcagt atttaattac cagcgacttg ttttgggtta agtttggtaa
    14161 tatggccact ttcagttcct gcagcagggt cgatgaccac attaataatg gtcggtttgc
    14221 gcgactgaat accggtggtt aaagcatgac gaagttcatc tgtcgtggtg acgttatagc
    14281 caacgccacg aaacgcatcc attaatttgt cataccttgc atggtgcaac agatccgttg
    14341 gtgatggtgc accagcgcca ctgagatcaa caccgtctcc tctgtagatg ccgccattat
    14401 taaaaataac gatcgtcacc ggcaggttat atcgacaaat cgtttcaatt tccatcccac
    14461 tgaaaccaaa agcactatca ccttcaatgg cgacaaccgg agaaccagaa gtcacgctag
    14521 caccgatggc atagcccata ccgatgccca tgacacccca ggtaccacaa tccagacgac
    14581 gacgtggttt atacatatca ataatatttc gtgcattatc cagggtattt gcaccttcat
    14641 taactaaata aatatcctgg ttctcgcgca atacgtcgcg cacagcactt aatgcattaa
    14701 agtaatttaa tggttgagta tctgtactta atttttcatg cattttttgt gcattttgct
    14761 gcttgtggat atttaaaata tcgcgccata ccagtggagt cgtaaatgtg ttttgtttca
    14821 gttctgccag cataccttgc atactggatg caatatcacc aacgactggc acagcaatgg
    14881 ggcggttgct gtcaatttcc tgcggttcaa tatccagttg aataaactgt gtatctgccg
    14941 cccatccttt tttaccgtgt gccaataacc aattcagtcg tgcaccaaca agcatgacaa
    15001 cgtcagcatt tgccagggca aacgaacgcg cagctgccgc agaaagtgga tgcgtatctt
    15061 caaggatccc tttcgccata gacattggca ggaatggaat ctgagcactt tcaataaatt
    15121 cacgaagctg ttcatcagct tgtgaatacg ccgcgccttt gccaaggata attaatggcc
    15181 gttcagcttt tgctaaaagc gaaattgcgc tagtgactga cttcgggcat ggcaataatg
    15241 ctggcgacgg attttcaact ttaacaatcg tggttaacgc ttcgtctttt tccatcgtcg
    15301 cggccaggac atttgctggc aaatcaagat aaactccgcc agggcgaccc gatacagaga
    15361 cccggatagc gcgtgccaat gcaatgccaa gatcctgcgg ctgattaacg cgaaatgctg
    15421 ctttggcata cggttttgcc gcattcattt ggtccagctc ttcataatca ccttgctgta
    15481 ggtcgacgat cgcgcggtcg ctggagccgc taatcatgat catcggaaaa ccatttaccg
    15541 ttgcgttggc caatgcggtc aaaccattga ggaatcctgg cgcagaaact gtcaggcaga
    15601 tccccggttt ttgggtaaga aaaccgcttg ccgcagcggc atagcctgcc gactgctcat
    15661 gacgaaaacc aatataacga atgccttccg cctgggcatg gcgtgccata tccgtcacag
    15721 gaatacctac aacaccataa atagtgtcaa tattattctg ttttaatgct tcaacgatga
    15781 tatgcatacc atctgtcatt tgaagttgat ctgacatgat taataacctc gaaattttta
    15841 taaattggga gccagaagcc cccgttaata tcagatggcg tggttttgct tcattgcagc
    15901 aatttcatcg tcgctataac ccagctcctg caatacagca gcggtatgtt cacctaatag
    15961 cggcgcagct ttaatatccg gcgtaaaggc agagaatttc attggacaac caacggtcag
    16021 atattttcca cgcaacggtt gttccacttc aacaacactg ccactttggc gcaaagaggg
    16081 atcaagtgaa atttctttca tacttaaaac cggtgcacaa ggaatatcaa actgagtcaa
    16141 ataggccacc gcttcatgtt tatcaatagt gacagtgtat ttttcgattt cagcaaaaat
    16201 atcgaaaata tgtggctgtc gggcatgggc tgtactgtat gccgaatcgg taatccattc
    16261 tggtttgccg atggctttac aggtgttttc ccagttttgc tcctgaatag tgaaataaat
    16321 ataggcgtta ggatcggttt cccagccttt acatttcagg atccagccag gctgaccgcc
    16381 accacctgca ttaccaccgc ggggaactgc atcaccaaat gtaccattcg gatactgcgg
    16441 gtattcttcc agataaccca atttatcgag acgctgctgg tcacgtaatt tcacgcggca
    16501 aaggttcaat acggcatcct gcattgacat ggtgactcgt tgcccacgcc ccgttttttc
    16561 gcgatgcagc aaagcagcaa gtaaaccgat cagcaaatgc attccggtgt tgctgtcacc
    16621 caacgctgca gcgcttacca gcggcggacc atcccaaaaa cccgtagtgg atgccgcgcc
    16681 acccgctgcc tgagcaacgt tttcataggc ttttacattc acataaggcg aacactcatc
    16741 aaaccctttg atcgaaccaa aaatcagacg tggattgatt tcttgaatat gctcccaggt
    16801 gaagcccatg tgatcaatgg cccctggatg aaagttctcg actaagatat cagcttcgcg
    16861 gatcagcttt tccattacct ctttgccttc cgctgttttg gtatttaact caatagaacg
    16921 tttgttactg ttaagcatgg tgaagtaaag cgcatcgata tcaggaatat ctcgcagttg
    16981 gtgacgcgtt acgtcaccaa cgccgggacg ttcaatttta ataacgtcag cgccaaacca
    17041 ggccagcatt tgagtacaag atgggccaga ttgcacaccg gtgaaatcga gaactttaat
    17101 tccttgaagt ggagttgaca tagcaatacc tattagttaa ttaaatatgt gttaatttag
    17161 cgtttattta aggcataacg ccctctataa ataatctgca aaatatcttc gtagcggcac
    17221 tcgatatttt taaattcctg tatcaagtac tgaaaattat ttcatcgcca cttccaattc
    17281 ctttctgcat ggtaagtagt gaggacgaaa agaagaatat gataaatatt tattcgtggt
    17341 aatcagagag agtcgcgaca tttgtgatgt tattcttaca actgtaagaa gcgccggtga
    17401 tttacaagta agagtatgat tacgcttttt aaagcgtacc ataaagcata acataataaa
    17461 tgatttgtga tgacactgga ttattgcggt ttactttgag gaaaataaaa tgcgtgttta
    17521 tagaaccgcg agcgccaaat aattttatga ccacaacctg tcgtattgtg gtcataaaaa
    17581 attgtcggct ggagggagtt aaatccttta cctcaatata ctgcctgtca gcattagttc
    17641 acaaacagtg cttcgctatc gacgatgatt ccatcttccg cacctttaag cgccaggttt
    17701 gcctcgtagt attgcttttt atcaagcagt ttctgcgcat cagcaagtgc attacgagtc
    17761 tgtttaagcg gcatcagata ttggttttcc attacgccaa caccggccag gcgaagctct
    17821 tccattgcgc cctttttatc ccctttcgcc attttttcat tggcaatttt tatcgcggct
    17881 tctttttcgg gtgttgctac ataactctca gaaattccga cagatgcatt aatgacaata
    17941 tattggtcat cattgagatt ggtcttttta cctggcttag cgaactttgc ccattcggta
    18001 ctgtcatctg acagcaaagc ggaagcttca ttggtcagtt cttttgcctt ttccggatcg
    18061 ccatgaaaca gagccaggcg cgctacctgc acgtcacgca tcgcatataa accttgttca
    18121 gagatacgct ctgctgctgc cgttttctgg gtttgctgag tttgctgttg tgccgctacc
    18181 ggtgcattat cagcagccca tacagttgaa gatgccagaa ttgctgtgac catcgtagcc
    18241 ataattaaac gtttcatact tacctcattc tttaacagtt ttagaaaacg ccgccatcat
    18301 attgggtgag catgaaatga gaaatcagag agcaacggtc attttgaaag ggttatctta
    18361 caattgtagt tattatcgca aagcggcatt tagtcttaca gttgtaggaa tatgcttcct
    18421 gtcgcagaga actattctta tataccacaa agagatgtca tttattatat tgatgtaaat
    18481 attgattaat tatatgtaaa tacagccata acatcgtatg gttctcagtg aaccattttc
    18541 aggataactt tctatgaaag taaacttaat acttttcagc ttatttttat tggtctctat
    18601 tatggcatgc aatgtttttg cattttccat ttctggtggt gtaagtgaga ggagctataa
    18661 agagactgaa aaaacatcag cgatgacaac cacacactct acaaaacttc agccatcaca
    18721 ggcgattttg tttaagatga gagaagatgc gccaccatta aacctcacag aagaaatcac
    18781 gcctacttat ccgacaaagg cgaattatct tattcatcct gtgcgatagc gtctgtcaca
    18841 tggcaggtga acaatcatgt ctgatgctga tgtgaacacc tgcccaaatg agccatcctg
    18901 acccactatc gcctgccata ccccaccata aagcgaatca tcaagccaca agccggggca
    18961 agaaacataa atatccccgt caccagttca ttaccgccag ttaaccacat ggtgaagtaa
    19021 taaatgagtg cagcgaggat caccatgccg aagaaagaag agaccgccga taccacgttc
    19081 agtacctgac cgatgtagcc gcttacgcag acaatacaca gggcgagaaa catccataaa
    19141 taaatcatag ctcaacctcc tgccgcgcta aaaaacctgt aacttcagtc actttaatag
    19201 taagacaatc cttaacctct ggcaattttt gctaagcatc aatgaaaaca gcacgttaaa
    19261 tgcaacgcta ttgcgtgcgg aaaatttttt actttgcacg attaataatc agtagctgaa
    19321 agcagtcagc cagtgataaa gtagagccac cgcattcatg ctcctcgtgg ttatgtcctg
    19381 actagtcttt acactcttta caggaaccat tgtcgtacat gatggcccaa ccaattgagt
    19441 gtaccgctgt cgacatggaa tcttctgcga tacaacaatt cgtatctaca gaaggtaact
    19501 atgtttccac aatgcaaatt ttcccgcgag tttctacatc ctcgctactg gctcacatgg
    19561 tttgggcttg gtgtactctg gctttgggta cagcttcctt atcctgttct ctgctttctc
    19621 ggcacgcgta ttggcgcaat ggcgcgacca ttcctgaaac gtcgtgaatc tatcgcccgt
    19681 aaaaacctgg aactttgttt cccgcagcat tctgcggaag aacgcgagaa gatgattgcc
    19741 gaaaactttc gttcactcgg catggcgctg gtagaaaccg gcatggcatg gttctggccc
    19801 gacagtcgcg tacgtaaatg gtttgatgtt gaagggttgg ataaccctaa acgcgcacaa
    19861 atgcaaaatc gcggcgtaat ggttgtcggc gtccatttta tgtcgctgga actgggcggc
    19921 cgcgtgatgg gactgtgcca accaatgatg gctacctatc gtccacataa taatcagctg
    19981 atggaatggg tgcagacccg tgggcgcatg cgctctaaca aagcgatgat cggcagaaat
    20041 aatctgcgcg gcattgtcgg tgcactgaag aaaggtgaag cggtatggtt tgctcccgat
    20101 caggattatg gtcgtaaagg cagctccttc gcgccgttct ttgcggtgga aaatgtcgcc
    20161 acaaccaatg gcacctatgt tctctcccgt ctctctggcg cagccatgtt gaccgtaacg
    20221 atggtaagaa aagcggatta cagcggatat cgtttgttca tcaccccaga gatggaaggc
    20281 tacccgacag atgaaaatca agccgctgcc tatatgaaca agattatcga gaaagagatc
    20341 atgcgcgcac cggagcagta cctctggatc caccgtcgct ttaaaacgcg cccggtggga
    20401 gaatcgtcgt tgtacattta acaaactgct cttactcttt ccctcctcaa atcggccata
    20461 ataacctcat ggtttttatg gcttttttct taaggttttt tccttcgctg aatatctaat
    20521 tattaccgtc agaaattaaa ctgtcgccga tatgagacac ttgtaagtta ctaaaagtaa
    20581 ataagaaaat gcctcttgtc tcgtttcagt tttaggcgta cattaacgcc gtcttgagca
    20641 gtttagctca gaattctggg gtgatgccca cggtaaaata cagggctttt aaggtcgctt
    20701 tgcccggtgt caactcactg tatcaggtgt aatgaagtca ttcaggcgta acagtaatta
    20761 cgcggagaga tgtaaagtga aatatttctt tatgggcatt tcttttatgg tcatcgtttg
    20821 ggccggtact tttgccctga tgatctaaaa gcaaactgtg caatgaaaaa caggagccat
    20881 caggctcctg tttgcatttc tgacgacggt gtttattccg cgttttcgtg aatatgtttg
    20941 ctgctggcgg gtagtaaacc gtcggcacgg aacatcgctt taatccctct gatggcctga
    21001 cgaatacggt cgcggttttc aatcagtgca aagcgaacat gcgtatcacc atagtcgcca
    21061 aagccaatcc ccggcgagac acaaaccttc gcttcgttaa ggagcttctt ggcaaattcc
    21121 agcgatccca tggccgcata tggttccggg attttcgccc agacatacat cgaagccttc
    21181 ggcatttcga ccatccagcc cgcttcatgc agccctttaa ccaatacatc acggcggcgt
    21241 ttgtactgtt cggtaatgtc gcgcacgcac tgttgatcgc cctcgagtgc cgcaatcgcc
    21301 gccacctgca acggcgtaaa agtgccgtaa tcgtgatagc ttttgattcg cgccagggca
    21361 ctgaccagcg ttttgttgcc gaccataaag ccgatacgcc agcccgccat gttgtagctt
    21421 ttcgacagcg taaagaactc gaccgccaca tcgcgtgcac ccggcacctg cataattgac
    21481 ggcgctttcc agccatcgta gacgatatcg gcataggcca ggtcatggac caccagcaca
    21541 tcgtagcgtt tcgccagcgc cacaaccttt tcgaagaact ccagttccac gcattgcgcg
    21601 gttgggttcg acgggaagcc gaggatcatc atcttcggtt tcggataact ttcacgaatg
    21661 gcgcgctcca gttcgttgaa gaaatcgaca ccttccacca gcggcactga gcgtacctgc
    21721 gccccggcaa tcaccgcgcc ataaatatgg atcgggtaac ttggattcgg caccagcacc
    21781 gtgtcaccat gatccagcgt cgccagcatc agatgcgcca ggccctcttt cgaaccaata
    21841 gtgacgatgg cttctgattc cgggtcgatt tcaacgtcgt agcgatcctg ataccagcgg
    21901 gaaatggcgc gacgtaaccg cggaatgccg cgtgaagtgg agtaaccatg cgtgtccggg
    21961 cgctgggcca cagtacataa tttttcgacg atatgcggcg gagtcgcacc gtccgggtta
    22021 cccatgctga aatcgataat atcttcgccg cgccgacgcg cagccatttt cagttcagcg
    22081 gtgatgttaa aaacgtaggg cgggagacga tcaatgcgcg taaagcgacg ttcagggcga
    22141 gtgtcagcca taatttcctc agattaacgt tagcgcccgg accgtccgag cgacgctgcc
    22201 acaatggtgg ctgttttgaa aatagcctga ttaatttctg tctgtccagc gttttagaac
    22261 atttttatca atggctttaa atgaggaact ccacttcaaa aacaggaaat ttttctac
//
LOCUS       DAZPLI010000058        21998 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZPLI010000058 DAZPLI010000000
VERSION     DAZPLI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.058"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..988)
                     /gene="cmtA"
                     /locus_tag="ACHUU3_002126"
     CDS             complement(<1..988)
                     /gene="cmtA"
                     /locus_tag="ACHUU3_002126"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFR2082192.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
     gene            complement(1016..1459)
                     /gene="cmtB"
                     /locus_tag="ACHUU3_002127"
     CDS             complement(1016..1459)
                     /gene="cmtB"
                     /locus_tag="ACHUU3_002127"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFR2082193.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            complement(1773..3764)
                     /gene="tkt"
                     /locus_tag="ACHUU3_002128"
     CDS             complement(1773..3764)
                     /gene="tkt"
                     /locus_tag="ACHUU3_002128"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR2082194.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            4042..4800
                     /gene="loiP"
                     /locus_tag="ACHUU3_002129"
                     /gene_synonym="yggG"
     CDS             4042..4800
                     /gene="loiP"
                     /locus_tag="ACHUU3_002129"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR2082195.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4943..5863)
                     /gene="speB"
                     /locus_tag="ACHUU3_002130"
     CDS             complement(4943..5863)
                     /gene="speB"
                     /locus_tag="ACHUU3_002130"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR2082196.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(6001..7977)
                     /gene="speA"
                     /locus_tag="ACHUU3_002131"
     CDS             complement(6001..7977)
                     /gene="speA"
                     /locus_tag="ACHUU3_002131"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR2082197.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7986..8117)
                     /gene="yqgB"
                     /locus_tag="ACHUU3_002132"
     CDS             complement(7986..8117)
                     /gene="yqgB"
                     /locus_tag="ACHUU3_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR2082198.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(8211..8354)
                     /gene="yqgG"
                     /locus_tag="ACHUU3_002133"
     CDS             complement(8211..8354)
                     /gene="yqgG"
                     /locus_tag="ACHUU3_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR2082199.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(8465..8716)
                     /gene="yqgD"
                     /locus_tag="ACHUU3_002134"
     CDS             complement(8465..8716)
                     /gene="yqgD"
                     /locus_tag="ACHUU3_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR2082200.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            8772..9926
                     /gene="metK"
                     /locus_tag="ACHUU3_002135"
     CDS             8772..9926
                     /gene="metK"
                     /locus_tag="ACHUU3_002135"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR2082201.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            10362..11756
                     /gene="galP"
                     /locus_tag="ACHUU3_002136"
     CDS             10362..11756
                     /gene="galP"
                     /locus_tag="ACHUU3_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR2082202.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11833..12330
                     /locus_tag="ACHUU3_002137"
     CDS             11833..12330
                     /locus_tag="ACHUU3_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR2082203.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            12425..13132
                     /gene="endA"
                     /locus_tag="ACHUU3_002138"
     CDS             12425..13132
                     /gene="endA"
                     /locus_tag="ACHUU3_002138"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR2082204.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            13212..13943
                     /gene="rsmE"
                     /locus_tag="ACHUU3_002139"
     CDS             13212..13943
                     /gene="rsmE"
                     /locus_tag="ACHUU3_002139"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR2082205.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13956..14906
                     /gene="gshB"
                     /locus_tag="ACHUU3_002140"
     CDS             13956..14906
                     /gene="gshB"
                     /locus_tag="ACHUU3_002140"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR2082206.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            15015..15578
                     /locus_tag="ACHUU3_002141"
     CDS             15015..15578
                     /locus_tag="ACHUU3_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR2082207.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            15578..15994
                     /gene="ruvX"
                     /locus_tag="ACHUU3_002142"
     CDS             15578..15994
                     /gene="ruvX"
                     /locus_tag="ACHUU3_002142"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR2082208.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16169..17149)
                     /locus_tag="ACHUU3_002143"
     CDS             complement(16169..17149)
                     /locus_tag="ACHUU3_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR2082209.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            17167..17871
                     /gene="yggS"
                     /locus_tag="ACHUU3_002144"
     CDS             17167..17871
                     /gene="yggS"
                     /locus_tag="ACHUU3_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR2082210.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17889..18455
                     /gene="yggT"
                     /locus_tag="ACHUU3_002145"
     CDS             17889..18455
                     /gene="yggT"
                     /locus_tag="ACHUU3_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR2082211.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            18452..18742
                     /gene="yggU"
                     /locus_tag="ACHUU3_002146"
     CDS             18452..18742
                     /gene="yggU"
                     /locus_tag="ACHUU3_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR2082212.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            18750..19343
                     /locus_tag="ACHUU3_002147"
     CDS             18750..19343
                     /locus_tag="ACHUU3_002147"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR2082213.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            19336..20472
                     /gene="hemW"
                     /locus_tag="ACHUU3_002148"
     CDS             19336..20472
                     /gene="hemW"
                     /locus_tag="ACHUU3_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR2082214.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            20783..21769
                     /locus_tag="ACHUU3_002149"
     CDS             20783..21769
                     /locus_tag="ACHUU3_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR2082215.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
     gene            21814..>21998
                     /locus_tag="ACHUU3_002150"
     CDS             21814..>21998
                     /locus_tag="ACHUU3_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR2082216.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGA"
BASE COUNT         5442 a         5719 c         5597 g         5240 t
ORIGIN      
        1 tagcaaagga caccagggta gctaccgtaa caccggcaat tgtcgccagg aacgagcctt
       61 tcggcgtcag tgccaggtaa gcaaagatag accccggact tgggccagcc accagaccac
      121 cgtccagtaa gttaaacacc caggtgccag acataccgcc cgcaatcatg gcaataatgg
      181 tcagcggctt catcagcaca tacgggaaat acagttcgtg gatcccaccg aggaagtgaa
      241 taatcatcgc cccgggcgca gaacgtttac tcatcccttt accaaacaag gtaaacgcca
      301 gcagcagccc taagccaggc ccaggattgg acgccaccat aaagaagatg gatttaccgt
      361 taaccgaagc ctgttgcatt cccagcggat aatagacgcc ctgatcgatc gcattattga
      421 ggaaaagcac tttcgccggt tcattgatga ctgacagcag aggcagataa cccgcatgta
      481 ccagcgcctc aatacactct ttgacgagag tattggcaat taacaccgcc gggccgataa
      541 cttcaaaacc cagcagacaa aggagcatcc ccgcgatacc taatgagaag ttattgataa
      601 ccatctcaaa accggcaggt atgcgttttt ccagtgcctt atcaacatat tttatgacca
      661 gcccaccgag cggccccata atcattgagc caaggaacat cgggatctct gcgccgacga
      721 tcacaccaat agtacctatt ccgcccatga ccgcgccgcg tttaccgccg accagatgac
      781 caccggtaga accaatcatc acgggcaata aataggtaat catcgggccg acaattttgg
      841 cgaaatgttc gttaggcagc caaccggtgg gaataaataa cgcagtaata aaaccccagg
      901 caataaaagc accaatattg gggatgacca ttgccgtcaa aaagccccca aaagcctgga
      961 cctttgcacg agcagacttg ttttccataa tattatcctg tagaggagag agtaattatc
     1021 cgcggctgat tatgtccgct aattgttttt ctgatgctgc tgttaagagt tgttcgagta
     1081 tttcttcttc gcacagtaat tcacttaacg cctgaatagc gccaatgtgc gaatcggcat
     1141 cggcagcaga aaggccgatg agtaatttta ttggttcgtc attccccgga aaataaacac
     1201 cttgttcaag taatgtcaaa gacatcccgg ttttaagcgc cccacattcc ggtcgcgcat
     1261 gaggcattgc cacgcctggt gcgagaatat aataagggcc attgttaatg gtggaatctt
     1321 taattgcctg aatgtaattc tcgctgatat agtttttatc cagcaatgat accatcgaga
     1381 aatcgatagc ttcctgccaa tcttttgctg aatgtataac tgagattgat gattctggaa
     1441 aatagtcact aagccgcata aattatcctt attttatttt tagggtaccg tccggcggta
     1501 aaaataaaat actcgtgcgg tttaatgaaa gcaattgagc agggaaaccg gtgttttgaa
     1561 aacagtgaat tattaatttc ttatataaca ttgagttata gatataacaa caaatgtcaa
     1621 tacgcatatc gtgatgcata ttacataatt gatatttatt gcatataaat atttgtgatc
     1681 tacaacacgc tttatctatt gcttgtccgc aaacggacat atcaaggtaa taaaaaaggt
     1741 cgccgaagcg acctttttac ccgaaatgct aattacagca gttcttttgc tttcgcaacg
     1801 acgttgtcga cggtgaagcc gaactcttcg aacagcaact ctgccggagc tgactcaccg
     1861 aaggtggtca taccgatgat agcgccattc aggccaacat acttgtacca gtagtcagca
     1921 atacccgctt ctaccgcaac gcgtgcagta accgctttcg gcagtacgga ttcacggtaa
     1981 gccgcatcct gcttgtcgaa tgcatcggta gacggcatgg aaaccacgcg cgccttcacg
     2041 ccttcggcag tcagtttttc gtaggcggca acagccagtt caacttccga accggtcgcg
     2101 atgaagatca gttccggctg accagcacag tctttcagca cataaccacc gcgcgcgatg
     2161 tttgccagtt gctcttcagt acgttcctgc tgcgccagat tctgacggga gaggataagc
     2221 gcggtcggac cgtcctgacg ctcaacaccg tatttccacg cgaccgcgga ttcaacctgg
     2281 tcacacggac gccatgtaga catgttcggg gtcacgcgca gagaagcgac ctgctcaacc
     2341 ggctggtgag tcgggccgtc ttcgcccaga ccgatggagt cgtgggtgta aaccatcacc
     2401 tgacgctgtt tcatcagcgc agccatacgt acagcgttac gtgcgtattc gacaaacatc
     2461 aggaaggtgg aggtgtacgg caggaagcca ccgtgcaggg agataccgtt agcaatcgcg
     2521 gtcataccga actcgcgaac accgtagtgg atgtagttac ccgcagcatc ttcgttgatt
     2581 gctttagaac cagaccacag ggtcaggtta gacggtgcca ggtcagcgga gccgccgagg
     2641 aattccggca acagcgggcc gaacgcttca atagcattct gagacgcttt acggctggcg
     2701 attttcgccg gattagcctg cagtttagcg ataaactctt tcgctttggc gtcgaagtca
     2761 gacggcattt cgcctttcat acggcgggta aattcagccg cttcctgcgg ataagctttc
     2821 gcgtaagccg caaacttctc attccatgca gattctttcg cctggcctgc ttctttcgca
     2881 tcccactgcg catagatttc agacgggatt tcgaacggtg cgtatttcca gcccagctgt
     2941 tcgcgggtca gagcgatttc tgcatcaccc agcggcgcac cgtgggagtc gtgagtaccg
     3001 gctttgttcg gggaaccgaa accgatgatg gttttgcaca tcagcaggga cggtttgtca
     3061 gtcactgcgc gcgcttcttc tactgcgcgt ttgatggatg ctgcgtcatg accgtcgatg
     3121 tcgcgaataa cgtgccagcc gtaagcttcg aaacgcattg cggtgtcgtc ggtgaaccag
     3181 ccttcaacgt gaccatcaat agagataccg ttgtcatcgt agaacgcaat cagtttaccc
     3241 agcttcagcg tacccgccag agagcaaact tcgtgggaga tgccttccat catgcagccg
     3301 tcgcccatga aggcgtaggt gtagtggtcg acaatgtcgt ggcccggacg gttaaactgc
     3361 gccgccagcg ttttttctgc aatcgccata ccgactgcgt tggcaatacc ctgacccagc
     3421 ggaccggtgg tggtttccac accagcggta taacctactt ccgggtggcc cggagtttta
     3481 gagtgcagct gacggaagtt tttcagttct tccatcggca gatcgtaacc ggtgaggtgc
     3541 agcaggctgt agatcagcat ggagccgtgg ccgttggaca gcacgaagcg gtcacggtca
     3601 gcccaggacg gattctgcgg gttgtgtttc aggaaatcac gccacaggac ttcggcaatg
     3661 tcagccatac ccataggggc acccgggtga ccggatttgg ctttctgtac tgcgtccatg
     3721 ctcagcgcac gaatagcatt ggcaagctct ttacgtgagg acattttgac tccagatcgg
     3781 atgatgaagg gcacgccctt aacgacttga cgacagcgcg ttttgggcta cgccggaaaa
     3841 tttgccaaca atttaccgca agccgcgcgt catgtacatg gaacatcctt ttgccgcttc
     3901 agaaatctct ggatcatgct cgcatgttgc gcaatctact cgcccgtccg ctgcgctttt
     3961 ccttatactg agactgagcg tcgattcacc tgcaaacggc gcatttttag aataatcctg
     4021 accttgtgcg gaagagaaaa catgaaaatt cgcgccttat tggtagcaat gagcgtggca
     4081 acggtactga ctggttgcca gaatatggac tccaacggac tgctctcatc aggagcggaa
     4141 gcttttcagg cttacagttt gagtgatgcg caggtgaaaa ccctgagcga tcaggcatgt
     4201 caggagatgg acagcaaggc gacgattgcg ccagccaata gcgaatacgc taaacgtctg
     4261 acaactattg ccaatgcgct aggcaacaat atcaacggtc agccggtgaa ttacaaagtg
     4321 tatatggcga aggatgtgaa cgcctttgca atggctaacg gctgtatccg cgtctatagc
     4381 gggctgatgg atatgatgac agataacgaa gtcgaagcgg tgatcggtca cgagatgggg
     4441 cacgtggcgt taggccatgt gaagaaaggg atgcaggtgg cgcttggtac aaatgccgtg
     4501 cgagtagctg cggcctctgc gggcgggatt gtcggcagtt tatctcaatc acaacttggt
     4561 gatctgggcg agaaattagt caattcgcaa ttctcccagc gccaggaagc agaagccgat
     4621 gattattctt acgatcttct gcgccaacgc ggcatcagcc cggcaggtct tgccaccagc
     4681 tttgaaaaac tggcgaaact ggaagaaggc cgccaaagct caatgtttga cgaccatccg
     4741 gcatccgccg aacgcgccca gcatattcgc gatcgcatga gcgcggatgg gattaagtaa
     4801 agcctggtgg tgtcgaacgt aggtcagata aggcgttcac gccgcatctg acatcaatgg
     4861 cacatttgtt accctgtgcg cattgccgga tgcgatgctg actgcatctt atccggccta
     4921 cgggtggcat cgcatctggt gcttactcgc cctttttcgc cgcttggata tacagcatct
     4981 ccagcgccag cgtcgccgca gccagagcgg tgatttccga ctgatcgtat gccggagcca
     5041 cttccactac gtccatccca acaatgttga gatctttcag gccgcgtacc agtttaatag
     5101 cgcgatcgga ggtcaggccg ccaatcactg gcgtaccggt gcctggtgca aaagcaggat
     5161 ccaggcagtc gatatcaaaa gtcaggtaaa ccggcatatc acccacaatc tgtttcactt
     5221 gggcgataac gtcatccacg ctgcgatcgt tcacctggca ggcgtccagc acggtaaagc
     5281 cgttgtcttt atcaaactcg gtacgaatac caatctgcac ggaatgattc gggtcgatca
     5341 gaccttcttt cggcgcggta tagaacatag tgccgtggtc aaattcacaa ccgttcgcat
     5401 aggtatcggt gtgggcgtca aagtgtacca gcgccatttt gccgaaatgc ttcgcatgag
     5461 cacgcagcag cggcagcgta acaaagtggt caccaccgaa ggtgagcata cgcttaccgg
     5521 cagccagcag cttctcggcg tgcgcctgca gcttttcgct catctcacgg gcatcgccaa
     5581 aggcatatac cagatcgccg cagtccacga cgttcagacg ctcacgcatg tcgaaattcc
     5641 acgggaagcg gttgtgttcc caggccagat tcgtcgaaac ctgacggatc gctgccggac
     5701 cgtggcgacc acccgcacga ccagaagtgg ccatatcgaa cggcacgcca gtaatcaccc
     5761 agtctgcatc gctgtcatac ggctggaagt tcatcggcag gcgtaaaaaa ccaaaggcat
     5821 tggaaaccag tgagttatcg tattgatgac ctaaggtgct catggatgga ctcctattac
     5881 aaagtcgata taaaaaaacc cttccgcgtc gttaggcccg acgaggaagg gttggatttg
     5941 tcacaataaa ttgtggcgga ttatcaccgc taaattaagc ggattcaagt aacacaggac
     6001 ttactcatct tcaagataag tataaccgta caaacctgcc tcgaactctt caaggaactg
     6061 ttgttgcagt tcagcatcaa gatcggtttt cttcacttga tcgcggaact gggttaacag
     6121 cgttttcgga tcgagctgta catattgcag catgtccgcc acggtatcgc cttcgtcaga
     6181 cagttctact tctacgctac cgtcagggaa cacgaacacg tcaaccgctt cggtatcacc
     6241 gaacaggttg tgcatattgc caaggatctc ctgatatgcg ccgaccataa agaaaccgag
     6301 catcggcgga ttctctggat cgtactccgg cattggcatt gtcgtggcaa taccatcacc
     6361 atcaatatag tggtcgatag caccgtcaga gtcacaggta atatccagca gcacagcgcg
     6421 acgttccggc acttgatcca gcccttccag cggcagaacc gggaacaact ggtcgatccc
     6481 ccatgcgtcc ggcattgact ggaacagcga gaaattgacg tacattttgt ccgccatacg
     6541 ttcctgcagt tcgtcgatta tcggacgatg cgcacggttt tgcggatcca gttgcttctg
     6601 cacttcatgg cacatgctca gatagagttg ttctgcccat gcacgttctt gcaggctaaa
     6661 ggtgccggaa gagtagccga tatgaatgtc gtgcagatcc atctggctgt cgtgtaacca
     6721 ttcacgcaaa gaacggcgag ttcccggttc gtgcatttcc tgccaggttt cccacatgct
     6781 ttgcagcgcg cgcggcgcat cttctgcggg cgcggtcggc accgtatatt cgttacgttc
     6841 cacgccgatg atattcgaca ccagcacggt atggtgcgca gtcaccgcac ggcccgattc
     6901 ggtgattacc gtcggatgcg gcagaccatt ttcttcacac gcatcgccaa tcgcccagat
     6961 gatgttgttg gcgtattcat tgaggccata gtttacggag cagtcagact gcgaacgggt
     7021 gccttcatag tccacgccca gaccgccgcc gacgtcgaag cactgaatat tgacgcccag
     7081 cttgtgcagt tccacataga aacgcgcgga ttcacgaacg cccgtggcga tatcgcgaat
     7141 attcgccatc tgcgaaccga ggtggaagtg cagtagttgc aggctgtcga gacgcccggc
     7201 ttcacgcagg gtttcaacca gttgcagtac ctgagtcgca gccaggccga acttcgattt
     7261 ttccccgccg gaggactgcc atttacccga gccctgcgaa gccagacgtg cacgcacgcc
     7321 cagacgagga acgacattca gacgttctgc ttcatccagc acaatggcga tttctgacat
     7381 cttctcaatg accagataga ccttgtgccc catcttctcg ccaattaatg ccaggcggat
     7441 atattcgcgg tctttataac cgttgcagac gatgacgcta cgggtcatgc cagcatgtgc
     7501 cagtactgcc atcaactcgg ctttggaacc ggcttccaga cccagcggtt cgcccgaatg
     7561 aatcagggac tcaatcacgc ggcggtgctg gttaactttg atcggataaa caaggaagta
     7621 atcgccgtta tagccgtagg attccctcgc acgtttgaac gcggcgttaa tggaacgcaa
     7681 acggtgctgc aggatctgtg ggaaacagaa cagtgcaggc agacgctgac cctgtgcttc
     7741 acgagttttc actaactgcg cgagatcgac gcgagcttcc gggacgtccg ggtccgggca
     7801 cacgctgatg tggcccagct cgttaacgtc atagtagtta ttgccccacc aggcaatatt
     7861 gtaagtacgc agcatcttgc tggcttcctg ggagctcatt gcaacctcct gcatggagcg
     7921 tagtacaccg tgttcgcccg ctgacgaagg caaacccata gacatgtcgt cagacatagc
     7981 gaacctcaaa ttattttatt aagtgtaaaa cagttaacga ctatcgcagc ctggaactgc
     8041 gataacaacc cataagcaca tggattttcc agcagtgaat gctgacgctc caactgcgca
     8101 accggtttct ttttcataac attattaagc acataaccga acgtaagtgt gacagttcgg
     8161 cgaaaccacg agaaaactct tgtttttaca agagcgccct tgttcagtcc tcagtaactg
     8221 taaccagctc ttgaatcctg agaagcgccg agatgggtat aacatcggca ggtatgcaaa
     8281 gcagagatgc agagtgcggg gaacgaatct tcaccagaac ggtgagacag gttaagcagc
     8341 agacaacggt tcattatttc gtatcacctc cacggccgcc tgttaagacg aacccacaag
     8401 ccaaaactct gatttcaacc cggctggaag tggcaacacg aaagaaacgt cgtgtgcttt
     8461 ttatttaagc cgcgcgccgc gttttatacc ccacaatggc agaaaattgc aaaagataaa
     8521 tacgcagaat gccggcattg tcaggaaaaa tttccagcca cgtttttaac agaatgagac
     8581 acgattcaaa aaaaagtgga aatcgggtga agaattgacc taaaatagcc atccagatgt
     8641 taatccatcc ataccgatta acactcagac tgccagtgtt tttaacctgc agagtcgtgg
     8701 taggatccgc taccacagaa aatccacaca acagtttgag ctaaccaaat tctctttagg
     8761 tgatattaaa tatggcaaaa caccttttta cgtccgagtc cgtctctgaa gggcatcctg
     8821 acaaaattgc tgaccaaatc tctgatgccg ttttagacgc gatcctcgaa caggatccga
     8881 aagcacgcgt tgcttgcgaa acctacgtaa aaaccggcat ggttttagtt ggcggcgaaa
     8941 tcaccaccag cgcctgggta gacatcgaag agatcacccg taacaccgtt cgcgaaattg
     9001 gctatgtgca ttccgacatg ggctttgacg ctaactcctg tgcggttctg agcgctatcg
     9061 gcaaacagtc tcctgacatc aaccagggcg ttgaccgtgc cgatccgctg gaacagggcg
     9121 cgggtgacca gggtctgatg tttggctacg caactaatga aaccgacgtg ctgatgccag
     9181 cacctatcac ctatgcacac cgtctggtac agcgtcaggc tgaagtgcgt aaaaacggca
     9241 ctctgccgtg gctgcgcccg gacgcgaaaa gccaggtgac tttccagtat gacgacggca
     9301 aaatcgttgg tatcgatgct gtcgtgcttt ccactcagca ctctgaagag atcgaccaga
     9361 aatcgctgca agaagcggta atggaagaga tcatcaagcc gattctgccc gctgaatggc
     9421 tgacttctgc caccaaattc ttcatcaacc cgaccggtcg ttttgttatc ggtggcccga
     9481 tgggtgactg cggtctgact ggtcgtaaaa ttatcgttga tacctacggc ggcatggcac
     9541 gtcacggtgg cggagcattc tctggtaaag atccatcaaa agtggaccgt tccgcagcct
     9601 acgcagcacg ttatgtcgcg aaaaacatcg ttgctgctgg cctggccgat cgttgtgaaa
     9661 ttcaggtttc ctacgcaatc ggcgtggctg aaccgacctc cattatggta gaaactttcg
     9721 gtactgagaa agtgccttct gaacaactga ctctgctggt acgtgagttc ttcgacctgc
     9781 gcccatacgg tctgattcag atgctggatc tgctgcaccc gatctacaaa gaaaccgcag
     9841 catacggtca ctttggtcgt gaacatttcc cgtgggaaaa aaccgacaaa gcgcagctgc
     9901 tgcgcgatgc tgccggtctg aagtaatctt tcttcacctg ctttcaaagg ccagcctcgc
     9961 gctggccttt ttcttttacc tcaccgcggt ggataggcat tcacgccgca tccggcaaaa
    10021 agctgcccgc acaataacat cattcttcct caacacgttt tatcgcaaat tatcatcact
    10081 actgaaaccg attacaccaa taacaccagc caaatatttg taatattttc atattattat
    10141 tcagttttca cagttgttac atttcttttc agtaaagtct taattgcaga taacagcgtt
    10201 taatctatga tgatataact caattatttt catgcactta aaccataact aagataaatg
    10261 ttagtgtaag cgattacact gatgtgattt gcttcacatc tttttacgtc gtactcacct
    10321 atcttaattc acaataaaaa ataaccatat tggagggcat catgcctgac gctaaaaaac
    10381 aggggcggtc aaacaaggca atgacgtttt tcgtctgctt ccttgccgct ctggcgggat
    10441 tactctttgg cctggatatc ggtgtaattg ctggcgcact gccgtttatt gcagatgaat
    10501 tccagattac ttcgcacacg caagaatggg tcgtaagctc catgatgttc ggtgcggcag
    10561 tcggtgcggt gggcagcggc tggctctcct ttaaactcgg gcgcaaaaag agcctgatga
    10621 tcggcgcaat tttgtttgtt gccggttcgc tgttctctgc ggctgcgcca aacgttgaag
    10681 tactgattct ttcccgcgtt ctactggggc tggcggtggg tgtggcctct tataccgcac
    10741 cgctgtacct ctctgaaatt gcgccggaaa aaattcgtgg cagtatgatc tcgatgtatc
    10801 agttgatgat cactatcggg atcctcggtg cttatctttc tgataccgcc ttcagctaca
    10861 ccggtgcatg gcgctggatg ctgggtgtga ttatcatccc ggcaattttg ctgctgattg
    10921 gtgtcttctt cctgccagac agcccacgtt ggtttgccgc caaacgccgt tttgttgatg
    10981 ccgaacgcgt gctgctacgc ctgcgtgaca ccagcgcgga agcgaaacgc gaactggatg
    11041 aaatccgtga aagtttgcag gttaaacaga gtggctgggc gctgtttaaa gagaacagca
    11101 acttccgccg cgcggtgttc cttggcgtac tgttgcaggt aatgcagcaa ttcaccggga
    11161 tgaacgtcat catgtattac gcgccgaaaa tcttcgaact ggcgggttat accaacacca
    11221 cagagcaaat gtgggggaca gtgattgtcg gcctgaccaa cgtacttgcc acctttatcg
    11281 caatcggcct tgttgaccgc tggggacgaa aaccaacgct aacgctgggc ttcctggtga
    11341 tggctgctgg catgggcgta ctcggtacaa tgatgcatat cggtatccac tctccgtcgg
    11401 cgcagtattt cgccatcgcc atgctgctga tgtttattgt cggttttgcc atgagtgccg
    11461 gtccgctgat ttgggtactg tgctccgaaa ttcagccgct gaaaggccgc gattttggca
    11521 tcacctgctc cactgccacc aactggattg ccaacatgat cgttggcgca acgttcctga
    11581 ccatgctcaa cacgctgggt aacgccaaca ccttctgggt gtatgcggct ctgaacgtac
    11641 tgtttatcct gctgacattg tggctggtac cggaaaccaa acacgtttcg ctggaacata
    11701 ttgaacgtaa cctgatgaaa ggtcgtaaac tgcgcgaaat cggcgctcac gattaatctc
    11761 ctcaggcttc ctcccgtcgc ggaggaagcc gcctcttgca gtcatctttt cttcgctcta
    11821 tcctctgccg ctatgaaaac atcccgtctc cctatcgcca tccaacaggc cgttatgcgc
    11881 ggcctgcggg aaaaactcgc ccaggccaac ctgaagctag ggcgtaacta cccggagcca
    11941 aaactctctt acacccagcg cggaacctcc gccggaacag cctggctgga aagctatgaa
    12001 atccgcctca atcccgtttt gctgttggaa aacagtgaag cttttattga agaagtggta
    12061 ccgcacgaac tggcacattt gctggtatgg aaacatttcg gccgcgtagc gccacatggc
    12121 aaagagtgga agtggatgat ggaaagcgtg ctgggtgttc ccgcccgtcg tacgcatcag
    12181 ttcgaactgc aatccgtgcg tcgcaacacc ttcccctacc gctgcaagtg ccaggagcat
    12241 cagcttaccg tacgccgcca taatcgcgta gttcgtggcg aggccgtcta tcgctgtgtt
    12301 cactgcggtg aacagctggt tgcgaaataa ccatctgaac tatcaggaac tttcctgatc
    12361 tggctgattg cataccaaaa caactttcgc tacgttgctg gctcgtttta acacggagta
    12421 agtgatgtac cgttatttgt ctattgctgc ggtggtactg agcgcagcat tttccggccc
    12481 ggcgttggcc gaaggtatca atagtttttc tcaggcgaaa gccgcggcgg taaaagtcca
    12541 cgctgacgcg cccggtacgt tttattgcgg atgtaaaatt aactggcagg gcaaaaaagg
    12601 cgttgttgat ctgcaatcgt gcggctatca ggtgcgcaaa aatgaaaacc gagccagccg
    12661 cgtagagtgg gaacacgtcg ttcccgcctg gcagttcggt caccagcgcc agtgctggca
    12721 ggacggtgga cgtaaaaact gcgctaaaga tccggtctat cgcaagatgg aaagcgatat
    12781 gcataacctg cagccgtcag tcggtgaggt gaatggcgat cgcggcaact ttatgtacag
    12841 ccagtggaat ggcggcgaag gccagtacgg tcaatgcgcc atgaaggtcg atttcaaaga
    12901 aaaagctgcc gaaccaccag cgcgtgcacg cggtgccatt gcgcgcacct acttctatat
    12961 gcgcgaccaa tacaacctga cactctctcg ccagcaaacg cagctgttca acgcatggaa
    13021 caagatgtat ccggttaccg actgggagtg cgagcgcgat gaacgcatcg cgaaggtgca
    13081 aggcaatcat aacccgtatg tgcaacgcgc ttgccaggcg cgaaagagct aacctacact
    13141 agcgggattc tttttgttaa cccctacccc acgcgtacaa ccgcgtgggg agacgacgcg
    13201 gatttttaac tatgcgtatc ccccgcattt atcatcctga accactgacc agccattctc
    13261 acatcgcgct ttgcgaagat gccgccaacc atatcgggcg cgtactgcgc atggggccgg
    13321 ggcaggcgtt gcaattgttt gacggtagca accaggtctt tgacgccgaa attaccagcg
    13381 ccagcaaaaa aagcgtggaa gtgaaggtgc tggaaggcaa gatcgacgat cgcgaatctc
    13441 cgctgcatat tcacctcggt caggtgatgt cgcgtggtga aaaaatggaa tttacgatcc
    13501 agaaatcgat cgaactcggt gtaagcctca ttacgccact tttttctgag cgctgcggcg
    13561 ttaaactgga tagtgaacgt ctgaacaaga agctccagca gtggcagaag attgcaattg
    13621 ctgcctgtga gcagtgtggt cgtaaccggg tgccggaaat ccgtccagcg atggatctgg
    13681 aagcctggtg tgcagagcag gatgaaggac tgaaactgaa tcttcacccg cgcgccagta
    13741 acagcatcaa tacgttgccg ttaccggttg aacgcgtccg cctgctgatt ggcccggaag
    13801 gcggtttatc ggcagatgaa attgccatga ctgcccgcta tcaatttact gatatcctgt
    13861 tgggacctcg cgttttgcgt acagagacaa ctgcgctcac cgccattacc gcgctacaag
    13921 tacgatttgg cgatttgggc taacggagaa gaataatgat caagctcggc atcgtgatgg
    13981 accccatcgc aaacatcaac atcaagaaag attccagttt cgctatgttg ctggaagcac
    14041 agcgtcgtgg ttacgaactt cactatatgg agatgggcga tctgtatctg atcaatggcg
    14101 aagcccgcgc ccatacccgc acgctgaacg tgaagcagaa ctacgaagag tggttttcgt
    14161 tcgtcggtga acaggatctg ccgctggccg atctcgatgt gatcctgatg cgtaaagacc
    14221 cgccgtttga taccgagttt atctacgcga cctatattct ggaacgtgcc gaagagaaag
    14281 ggacgctgat cgttaacaag ccgcagagcc tgcgcgactg taacgagaaa ctgtttaccg
    14341 cctggttctc tgacttaacg ccagaaacgc tggttacgcg caataaagcg cagctaaaag
    14401 cgttctggga gaaacacagc gacatcattc ttaagccgct ggacggtatg ggcggcgcgt
    14461 cgattttccg cgtgaaagaa ggcgatccaa acctcggcgt gattgccgaa accctgactg
    14521 agcatggcac tcgctactgc atggcacaaa attacctgcc agccattaaa gatggcgaca
    14581 aacgcgtgct ggtggtggat ggcgagccgg taccgtactg cctggcgcgt attccgcagg
    14641 ggggcgaaac ccgtggcaat ctggctgccg gtggtcgcgg tgaacctcgt ccgctgacgg
    14701 aaagtgactg gaaaatcgcc cgtcagatcg ggccgacgct gaaagaaaaa gggctgattt
    14761 ttgttggtct ggatatcatc ggcgaccgtc tgactgaaat taacgtcacc agcccaacct
    14821 gtattcgtga gattgaagca gagtttccgg tgtcgatcac cggaatgtta atggatgcca
    14881 tcgaagcacg tttacagcag cagtaaccca ccttagcgag aaggatctcg ttgagactct
    14941 gagtgacagc gcccttcttt ccacgcatac tgggcgctgt tgcttttttg aaccaggaaa
    15001 cagaacctct gacaatgaat ttacagcatc actttcttat tgccatgcct gctctccagg
    15061 atccgatttt ccgtcgttcc gtggtctaca tttgcgaaca taataccaat ggtgcaatgg
    15121 ggatcatcgt caacaagccg ctggaaaatc tcaaaattga agggattctg gaaaagctga
    15181 agatcacgcc ggagccgcgt gatgaatcaa tccgtctgga taaaccggtt atgctcggcg
    15241 gtccgctggc tgaagatcgc gggtttattt tgcatactcc gccctccaat tttgcttcca
    15301 gcattcgcat ttcagacaac acagtgatga ccacttcccg cgatgtgctg gaaacgctcg
    15361 gcaccgataa acaaccgtct gacgtattgg tggctctagg ttatgcctcc tgggaaaaag
    15421 gtcaactgga acaagaaatt ctcgataacg cgtggctaac ggccccggcg gatctgaata
    15481 ttctgttcaa aacgccgatt gccgaccgct ggcgcgaggc ggcaaaactg attggtgtgg
    15541 atattctcac catgcctggt gtggcagggc acgcctgatg agtggaacct tactcgcctt
    15601 cgacttcggc accaaaagca ttggcgtagc ggtcggccaa cgcattaccg gcactgctcg
    15661 ccctttgcct gcaattaaag cacaggacgg tacgccggac tggaacctca tcgaacgttt
    15721 actgaaagag tggcagccgg acgaaatcat cgtcggtttg ccgctgaata tggacggcac
    15781 cgagcaacca ttgactgcca gagcgcgtaa atttgccaac cgtattcatg gtcgtttcgg
    15841 tgttgaagta aagctccatg acgaacgtct tagcaccgtg gaagcccgtt ccggtctgtt
    15901 tgaacagggc ggctatcggg cgctcaacaa aggcaaagtc gactctgcct ctgcggttat
    15961 tattctcgaa agctatttcg agcagggata ttaaggcgat ttaaaagcct ggcggatagt
    16021 cgttaccgcc atcttgccgg atgcggcgtg aacgcctatc cggcctacat gtggcagcga
    16081 ttgtaggtct gataagacgc gcaaacgtct catcagacgt ttattgcctt tgtaggcccg
    16141 ataagcttgc acatcgggca tggcaacgtc acaaacgccc ttcccccact cgctgctgaa
    16201 aactctgctg aaacgttatc atccccacct gctgcccggt ttgaataaca tgcggtaact
    16261 ggtgggtttt cccttcgcga atcaaattcc ccaccgcggg agtgttaatc aataattcaa
    16321 acagcgccac gcgtccttcc tgtttatcca cttccagctt ttgtgacaac acggcccgta
    16381 aactacctgc cagttgatta cgtacgggat ctttttcctg cgccggaaat gaatccacca
    16441 gtcgctcaac tgcctgcgcg gcaccacgcg tatgtaatgt tgccagcacc aaatgcccgg
    16501 tttctgccgc cgtcagtgcc agacggattg tctcgctgtc acgcagctct ccgagcaaaa
    16561 tcacatcagg atcttcccgc aatgcggccc gcaatcccga tgcgaacgtc atacagtgca
    16621 aaccaatttc ccgcttctgg atcaaacatc gctggctggc atagatatat tcaacaggat
    16681 cttccagcgt cagaatatgc gcatcggcat gttgattgag atagccaacc atcgccgcca
    16741 gcgtggtaga tttgctgctc cccgtcgccc ccgtcaccag aatcaggcca ttctcactct
    16801 tgagtaattc cggcaatacc gttggtgcgc caagctgttc gagctgcggg cagtgcgaag
    16861 gtaacagccg taacgccagc gaaatgccat gccgttgcgc aaatgcgctg ccacgcaatc
    16921 gctggttttc cgccagcgac acggcaaaat ccagctggcc attttccagc aatattgccc
    16981 gctgatcgtc atccagccac tcccgcagta gctcttcgac gtccggcgcc tcaaacggcg
    17041 cagcttccat tagcccgcga atgcgccatc gtgcgggcca ggcgctgcac aggtgtagat
    17101 ccgagacgtt atgctttaca ctaagggcca caatttcttc catattcata ctaagatcct
    17161 cggaaaatga acgatattgc gcataacctg gcacaggtcc gggacaaaat ctcagcggct
    17221 gcaacgcgtt gcggccgttc tccagaagaa attacgctgc ttgcagtcag taaaacaaaa
    17281 cctgcgagcg ccatcgcaga agccattgat gccgggcagc gtcaatttgg tgaaaactac
    17341 gttcaggaag gggtagataa aattcgccac tttcaggaac tgggcgtaac aggattagaa
    17401 tggcatttta ttggcccgtt gcagtctaat aaaagccgcc tggtggcaga gcatttcgac
    17461 tggtgtcata ccatcgaccg tttgcgcatt gctacccgcc tcaacgatca gcgcccggca
    17521 gaacttcccc ctcttaacgt actgattcaa attaatatta gtgatgaaaa cagtaagtcc
    17581 gggattcaac tggcagaact ggacgagctg gcagcggcgg tcgctgaact accgcgttta
    17641 cgtctgcgcg ggctgatggc aatccctgcg cctgagtcag aatatgtaag gcagtttgaa
    17701 gttgcacgcc aaatggctgt agcatttgcc ggactgaaaa cgcgctaccc gcatatcgac
    17761 acgctctctc tgggaatgtc ggacgatatg gaagccgcca ttgcggcagg tagcacgatg
    17821 gttcgtatcg gcactgcaat ttttggtgcg cgtgattact ctaaaaaata aggaattaaa
    17881 ggaacgccat gaatacgttg actttcctgc tttcaacggt cattgagctg tataccatgg
    17941 tgctgttatt acgcatctgg atgcagtggg ctcattgtga tttttacaac cccttctcac
    18001 agtttgtagt gaaggtaacg cagccaatta tcgggccact gcgccgcgtt attccggcaa
    18061 tgggccccat tgacagcgcc tcgctgctgg ttgcctatat tctcagtttt atcaaagcca
    18121 tcgtgctgtt taaagtggtg accttcctgc caatcatctg gattgctggt ttactgattc
    18181 tgctgaaaac catcggcctg ctgattttct gggtcctgct ggtgatggcg attatgagct
    18241 gggtaagcca gggtcgtagt ccgattgaat acgtgctgat tcagctggcc gatccgctgc
    18301 tgcgcccgat tcgccgcctg ctaccggcaa tgggtgggat tgatttctcg ccgatgatcc
    18361 tcgttctgct gctgtatgtc atcaatatgg gtgtcgcaga agtattacag gcaaccggaa
    18421 acatgctgct gccggggctg tggatggcgt tatgagtgcc gtaacagtta atgatgacgg
    18481 tctggtttta cggctctata ttcagccgaa agccagccgt gattctattg tcggtttaca
    18541 tggcgacgaa gttaaagtcg ccattaccgc gccgccggtt gacggccagg gcaacagtca
    18601 tctggtgaag tttctcggta agcaattccg ggttgccaaa agccaggtgg tgattgaaaa
    18661 aggcgaactt ggccgccaca aacaaattaa aatcattaat ccgcaacaaa tcccgccaga
    18721 aatcgcggcg ttaattaatt aggtatccta tgcaaaaagt tgtcctcgca accggcaatg
    18781 ccggtaaagt gcgtgagctg gcgtcgctgc ttagcgactt cggtcttgat atcgtggccc
    18841 aaacagacct cggcgtagat tccgctgaag aaaccggcct gacctttatc gaaaacgcga
    18901 ttctgaaagc gcgctatgcg gcaaaagtga ccggtttacc ggcaattgcc gacgactctg
    18961 gtctggcggt agatgcgctg ggtggcgcgc cgggtattta ctccgcgcgt tattccggtg
    19021 aagacgcgac cgatcaaaag aatctgcaaa aactgttgga aacactgaaa gacgtaccgg
    19081 acgaccaacg tcaggcgcgt ttccactgcg tgctggtata tctgcgtcac gcggaagatc
    19141 cgactccgct ggtatgccac ggcagctggc cgggcgtgat tactcgcgaa ccagcgggca
    19201 ctggtggctt tggttatgat ccaatcttct tcgtaccttc cgaagggaaa accgccgccg
    19261 aactgacccg cgaagaaaag agcgccattt cccaccgtgg tcaggcgttg aaactgctgc
    19321 tggacgcttt acgtaatggt taaattaccg ccgctgagtc tctacattca catcccgtgg
    19381 tgcgtgcaga aatgcccgta ctgcgatttc aactctcacg cgttgaaagg agaagtgccg
    19441 cacgacgatt atgttcagca tctgctttgc gatctggaca atgatgtggc ttacgctcag
    19501 ggccgtgaag taaagacaat ctttattggc ggtggtacgc cgagcctgct ttccggcccg
    19561 gcgatgcaaa cgctgctgga cggcgtgcgt gcgcgtttgc cgctggcagc ggatgcagaa
    19621 attactatgg aagcgaaccc tggaacggta gaagccgatc gctttgtcga ttatcagcgt
    19681 gctggtgtga accgcatctc tattggtgtg cagagtttta gcgaagaaaa gctgaaacga
    19741 cttgggcgta ttcatggccc gcaagaagcg aaacgcgcgg caaagctggc gagcgggctg
    19801 gggctgcgta gctttaacct cgatttgatg catggactac cggatcaatc actggaagag
    19861 gcgcttggcg atctacgcca ggccattgaa ctgaatccgc cgcatctttc ctggtatcaa
    19921 ctgaccatcg aacccaatac gctgtttggt tcgcgaccac cggtgctgcc ggacgatgac
    19981 gcgctgtggg atattttcga acaggggcat cagttattaa ccgcagcggg ttatcagcaa
    20041 tatgaaactt ccgcttacgc caaacccggt tatcagtgcc agcacaatct caactactgg
    20101 cgatttggtg actacatcgg tattggctgc ggcgcgcacg gcaaagtcac cttcccggat
    20161 gggcgcattc tgcgtaccac taaaacgcgt catcctcgtg gttttatgca aggaaggtat
    20221 ctggaaagcc agcgtgatgt cgaagccgca gataagccgt ttgagttctt tatgaatcgc
    20281 ttccgtctgc tggaggccgc gccgcgcgtg gagtttattg cgtataccgg gctttgcgaa
    20341 gatgtgattc gcccacagtt agacgaggcg attgcccagg gttatctcac cgaatgtgcg
    20401 gattactggc agataaccga gcacggtaaa ttattcttaa actcacttct tgaattattc
    20461 ctcgctgagt aatcattaat gcgcctgcgg gcgcattaat cacttctttt ccccccgtta
    20521 attaacttcg gcaaaatata tttcttactg aataaacatc gatgatattt attagatata
    20581 tctagtgaat atttactcat catttattga ttataaacat gtacaacctg gcgcacaaat
    20641 ttaatattta atttctgact ttatttcaag ttgtgataaa catagacaag ttgtcagaca
    20701 ggttaaccaa tttgtcgcac aaagcactga taagcacaat tagatatcaa ccaatttcca
    20761 tccttcaaca gggagttttt ttatgaaaat gaggtcattt acccgctcat tagtctgcgc
    20821 atccttgctg gctctggtta gcactggcgt aaacgccgca gaaaaagtga cgttaaagtt
    20881 agcacacaac ctggaaagaa gtcatgttgt acatcagagt tttgaagagc tggcgaaaga
    20941 ggtaaaacag ctgtcagaag gtaatatggt gatccgtatt tatcccagca gccagatggg
    21001 taatgcgcga gaaactatgg aattgttgca aaatggcgca ctggatatga ccaaaggttc
    21061 cgccagtgat ctggagtctt ttgataatat ttatgctatc tataatttgc cgttcttatt
    21121 taaagatcag gctcacttca acaaagttgt ttttggtgaa gttggtaaag aaattatgga
    21181 ctcaacaaaa gacaaaggct tttttgcttt atcagcttat gtagcgggta cacgcagttt
    21241 ctatgcgaaa aaaccgatta ctaagccaga agatcttaaa gggctgaaaa ttcgtgttca
    21301 gccaagtccg accaccatca aaatgattga actgatgggt ggttcaccca ctccaatttc
    21361 atttggcgaa gtttatactg cgatgcagca aggtgtagta gatggcgcag aaaataacgt
    21421 gccttcctgg gtgcagactc gacatattga aattgccaaa gttttttctg aagatgaaca
    21481 cgcctcaatt cctgactttt tggttatctc caccaaaaca tggaataaat taaccccaga
    21541 acagaaacaa atcctcgaaa cagcagcgaa gaaatccgaa gcttatcaac aaaaattgtg
    21601 ggaaaaaatt gacgctgaca cacgtgctca ggcgaaagct atggggggtg aaattgtaaa
    21661 agtggataaa gcaccattcc gtgccgcagt tcagcctctg tttgacgact tcaaaaaaga
    21721 tccaaaacaa gctgctttgt tagagaagtt cgacaacgct gctcaataat attccgggcc
    21781 agtctctggc cctgattttc caagatgctt acaatgatat ttaaccgcct gaaacttgcg
    21841 gtagaccgcg tcattgccgc gttttctgtc gccgtcatgc ttgctctggt tgtatgcgta
    21901 gtctggcaag tatttagccg ctacgtactt aaccagccaa gtacattaac cgatgagctg
    21961 gcacgatttt tgatgatctg ggtagggtta ttaggtgc
//
LOCUS       DAZPLI010000059        21642 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZPLI010000059 DAZPLI010000000
VERSION     DAZPLI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21642
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.059"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..1126)
                     /gene="fsr"
                     /locus_tag="ACHUU3_002151"
     CDS             complement(<1..1126)
                     /gene="fsr"
                     /locus_tag="ACHUU3_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR2082217.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1344..2996
                     /gene="ushA"
                     /locus_tag="ACHUU3_002152"
     CDS             1344..2996
                     /gene="ushA"
                     /locus_tag="ACHUU3_002152"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR2082218.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3033..3512)
                     /gene="ybaK"
                     /locus_tag="ACHUU3_002153"
     CDS             complement(3033..3512)
                     /gene="ybaK"
                     /locus_tag="ACHUU3_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR2082219.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3716..4510)
                     /locus_tag="ACHUU3_002154"
     CDS             complement(3716..4510)
                     /locus_tag="ACHUU3_002154"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR2082220.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4648..4989
                     /locus_tag="ACHUU3_002155"
     CDS             4648..4989
                     /locus_tag="ACHUU3_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR2082221.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5204..7708)
                     /gene="copA"
                     /locus_tag="ACHUU3_002156"
     CDS             complement(5204..7708)
                     /gene="copA"
                     /locus_tag="ACHUU3_002156"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR2082222.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7970..8902
                     /gene="glsA"
                     /locus_tag="ACHUU3_002157"
     CDS             7970..8902
                     /gene="glsA"
                     /locus_tag="ACHUU3_002157"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR2082223.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8905..10197
                     /locus_tag="ACHUU3_002158"
     CDS             8905..10197
                     /locus_tag="ACHUU3_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR2082224.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10228..10325
                     /locus_tag="ACHUU3_002159"
                     /pseudo
     CDS             10228..10325
                     /locus_tag="ACHUU3_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10322..10729
                     /gene="cueR"
                     /locus_tag="ACHUU3_002160"
     CDS             10322..10729
                     /gene="cueR"
                     /locus_tag="ACHUU3_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR2082225.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10730..11188)
                     /locus_tag="ACHUU3_002161"
     CDS             complement(10730..11188)
                     /locus_tag="ACHUU3_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR2082226.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11185..12102)
                     /locus_tag="ACHUU3_002162"
     CDS             complement(11185..12102)
                     /locus_tag="ACHUU3_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR2082227.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12248..12925
                     /gene="fetA"
                     /locus_tag="ACHUU3_002163"
     CDS             12248..12925
                     /gene="fetA"
                     /locus_tag="ACHUU3_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR2082228.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12912..13691
                     /gene="fetB"
                     /locus_tag="ACHUU3_002164"
     CDS             12912..13691
                     /gene="fetB"
                     /locus_tag="ACHUU3_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR2082229.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13754..14608)
                     /gene="cnoX"
                     /locus_tag="ACHUU3_002165"
     CDS             complement(13754..14608)
                     /gene="cnoX"
                     /locus_tag="ACHUU3_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR2082230.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14669..15478)
                     /gene="ybbO"
                     /locus_tag="ACHUU3_002166"
     CDS             complement(14669..15478)
                     /gene="ybbO"
                     /locus_tag="ACHUU3_002166"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR2082231.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15468..16091)
                     /gene="tesA"
                     /locus_tag="ACHUU3_002167"
     CDS             complement(15468..16091)
                     /gene="tesA"
                     /locus_tag="ACHUU3_002167"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFR2082232.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16062..16748
                     /gene="ybbA"
                     /locus_tag="ACHUU3_002168"
     CDS             16062..16748
                     /gene="ybbA"
                     /locus_tag="ACHUU3_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR2082233.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16745..19159
                     /gene="ybbP"
                     /locus_tag="ACHUU3_002169"
     CDS             16745..19159
                     /gene="ybbP"
                     /locus_tag="ACHUU3_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFR2082234.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19393..20487)
                     /gene="mnmH"
                     /locus_tag="ACHUU3_002170"
     CDS             complement(19393..20487)
                     /gene="mnmH"
                     /locus_tag="ACHUU3_002170"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR2082235.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20556..21482)
                     /gene="allS"
                     /locus_tag="ACHUU3_002171"
                     /pseudo
     CDS             complement(20556..21482)
                     /gene="allS"
                     /locus_tag="ACHUU3_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5013 a         5686 c         5800 g         5143 t
ORIGIN      
        1 gatcggcgat aagccccaga actgccgctc ccagacctcc catgccaaaa gcaaaaccga
       61 aaaagagtcc agaaaccata ccgatacgtc ccggaagtag ctcctgagcg tagaccagaa
      121 tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc cccgtccagt
      181 gcaggctggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata gagccccaaa
      241 tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta cctgccgcaa
      301 cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat ccgaattttt
      361 gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat ttcgagaaaa
      421 tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt ggattgatta
      481 tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa cggctaattt
      541 gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg ttgcctttgc
      601 cataaggcgc gataatcacc gccgccagca agggtcccag ggaactgcca aagttgccgc
      661 cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata cgggctacgc
      721 gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgcc gccagcagaa
      781 ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc acttaaggta aagcacatgc
      841 caattggcaa cgaccacggc atcggatatt tatcggtcca gtagccgacc actggttgca
      901 gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata aatgtcagag
      961 aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc atgtcgttca
     1021 gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc ttggtcgttg
     1081 acgcagccgc gcccgccaca ggctggggtt gttcactcat tgccatagga aagtcacttt
     1141 ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact aacatacctg
     1201 tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct ggatgcagat
     1261 ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt tcaacacgtt
     1321 gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg ttagcgctgt
     1381 taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat aaaacctaca
     1441 aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat gaatatggcg
     1501 aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag gttgcggctg
     1561 aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg cccgagtctg
     1621 acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat gacgcgatgg
     1681 cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag gaaaagtggg
     1741 ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag cgcctgttta
     1801 aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg ctgacaaccg
     1861 atgacacagc aaaaattggt aacccggaat acttcactga tatcgaattt cgtaagcccg
     1921 ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca gacattatta
     1981 tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac gcaccgggcg
     2041 atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc ggtggtcact
     2101 cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac gtgccgggta
     2161 cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat gagtggggca
     2221 aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg gttaactacc
     2281 agctgattcc ggtgaacctg aagaagaaag tgacctggga agacgggaaa agcgagcgcg
     2341 tgctgtacac ccctgaaatc gctgaaaacc agcaaatgat ctcgctgtta tcgccgttcc
     2401 agaacaaagg caaagcgcag ttggaagtga aaataggcga aaccaatggt cgtctggaag
     2461 gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt ctggcagccc
     2521 aaatggatcg cactggtgcc gactttgcgg tgatgagtgg aggaggaatt cgtgattcta
     2581 tcgaagcagg cgatatcagc tataaaaacg tactgaaagt gcagccattc ggcaatgtgg
     2641 tggtgtatgc cgacatgatc ggtaaagagg tgattgatta cctgaccgcc gtcgcacaaa
     2701 tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg gcgaaagacg
     2761 gcaaactcaa cgacctgaaa atcaaaggcg aaccggtcga tccggcgaaa acttaccgta
     2821 tggcgacatt aaacttcaat gccaccggcg gtgatggcta tccgcgcctt gataacaaac
     2881 cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat atccagaaaa
     2941 gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg cagtaatccg
     3001 aaagtgccgg atatttgcat ccggcacaat gcttaatcgc ggcgggcgat atcagcaaat
     3061 ttggcatcga ggatcttcgc cagatcgcct gccgccagtt cgatatccag tccgcgcttg
     3121 ccgccagaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat cgtcggcaga
     3181 cgttttttct gccccagtgg gctaatcccc ccaaccaggt atcccgtcga acgctgcgcg
     3241 gccatcggat cggccatctc aactttcttg gcacccagcg cttttgctac ttttttaaga
     3301 tccagttgac ctgcgaccgg cgtaacggcc actgccagat gtttcatatc accgttcact
     3361 gccaccagta gcgttttgta gacttgatcc ggattcaaac ctaatttttt gacgacttca
     3421 tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa cgaaatcttg
     3481 tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta aacaaaaaaa
     3541 cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc taagagtatt
     3601 ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt gacggcataa
     3661 taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca tttttttagc
     3721 gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc gcgaccacat
     3781 atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc tgatgcatca
     3841 gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta ttttgcggag
     3901 gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta tgccagtggg
     3961 tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac atggtaatct
     4021 ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc gcctgcaata
     4081 actggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc gtcgcctgaa
     4141 gaaccatcgc gatttgccac agcggttgag tagaaaagag cgacggagaa atgcccatct
     4201 cctggctaat gtgctgcaga ttttggagtt gctcctcgtt aatgcgctct tccagcgcct
     4261 cgcaagtagg caaattagca aaaggcgtat cgctggtgga aacatccgcc tcgacgatca
     4321 gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc atatcgtggc
     4381 tacccatatg aatactgcca atcagatgaa aatggcgatt gccgggaagg gtgatatcga
     4441 tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt acccggtaca
     4501 acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga gggatggcgc
     4561 aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct tgcatcgcta
     4621 ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc gacgacaccc
     4681 ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa tgagttagca
     4741 gagttgctgc atgttcatcg taatagcgtc agtgcactga tcaataacaa tcgtaaactc
     4801 accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga ttttaggcta
     4861 aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac ccaggaagaa
     4921 ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg tgcaaaaaag
     4981 gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt cacgccgcat
     5041 ccggcatccg agcaccattg cctgatgcga cgcttacccg tcttatcagg cctacaaacc
     5101 tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc atgcgtcgat
     5161 gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc ttcggtttaa
     5221 accgcagcaa ccggttggcg ttactcacta cggtaatcga cgagagcgcc attgccgctc
     5281 cggcaactac cgggttaagc agtgttcccg taaacggcca caaaataccg gcggcgaccg
     5341 gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg ttgcgcagcg
     5401 ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc atcagggtaa
     5461 tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg acatccgcct
     5521 gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga cgtccttcac
     5581 tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca atcacttcat
     5641 caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg gtcaacatca
     5701 ccagacgata tcccgtttta tgcaggcgtt gcagcgccgc cacgctatca ctacgcaacg
     5761 gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc acaggcgttg
     5821 ctccttgcga tgcctgagca gtaatctccg cttcgatagc tttggtacca acctgttgct
     5881 catttaacag cgcctgattg cccagcaata acgcatgacc ttcagcttca ccgctcacgc
     5941 ccagcccgcg caatgtgcgg aaaccgttga cctgcggcag ctgcatatca cctgctttat
     6001 cgaggatcgc ccgtgccagc ggatggctgg aaccttgctc cagtgccgcc gccagacgca
     6061 atgcctgcgc ttcatcaaca tcagcaaacg ttttcactgc gacaacctgc ggcttccctt
     6121 cggtcagcgt cccggtttta tcgaacacga cggtatcgag tgtactggcg cgttgcagcg
     6181 cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg gaaataatcg
     6241 acatcggcgt cgccagcccc agcgcacacg gacaggcgat aatcagtacc gtggtggcaa
     6301 tcaccagggt ataaacaatt tgcggtgccg gaccaaagaa gtaccagatt gccgcactga
     6361 ccagcgcaat caccaccacc acaggtacaa atacggctga gattttatcc gccagctgac
     6421 cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatccgt gacagcgtgg
     6481 tatggctgcc aaccgcactg gcacgaaaca gcacactgcc gtcctgtacc actgtcccgg
     6541 catggacgct ttcgccttcg cctttttgct gtgggattgg ttcgcccgtc agcatcgctt
     6601 catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga tcgccggtcg
     6661 tcaggcgcag taacatccct ggctgaactt ccgccagcgg cacgcttttt tcaccatctg
     6721 cggtgaccac tcgcgccgtc ggtggggtta aatcgagtaa tttctccagc gccttagaag
     6781 aacgctgacg tgcgcgcgct tccagcatat ggccgagatt aatcagaccg ataatcatcg
     6841 cactggcttc atagtaaaga tgccgcgctt ccatcgggaa ccactgcggc cacaggttga
     6901 cgctcatcga atagagccac gccacacccg ttcccagcgc caccagcgta tccatcgtcg
     6961 ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgccg gcgaaaacca
     7021 tcactgccag ggttatcagg ccgataacta accacaagct gcggttgtca gcggtgacca
     7081 tcatgttatc gccgatcatc ccccagacca ttaccgggat acccacagcc agtgcaacaa
     7141 ttgcctgcca gcggaagcgc ttcatcgtag ctacggcggt ttcttgctgg cgctcgcggc
     7201 gtttagcgtc atcttcaatc gcttccgcac cgtagcccgc tttttccacc gcctgcacta
     7261 aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg tttacccgtg
     7321 cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca cagctggcgc
     7381 agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc gctgccggaa
     7441 gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc agcggtttag
     7501 cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg atcagctgtt
     7561 ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgcc tgctcaacat
     7621 ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag gacaggccgt
     7681 ccagggtcag gtcgatagtt tgtgacataa aacactcctt taagacagtt ttgactggct
     7741 gtgataaagg ttaaaccttc cagcaagggg aaggtcaaga aattaataaa ccaggcgggt
     7801 aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac atttatttct
     7861 ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg aaaatcggca
     7921 ccggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga tgttagatgc
     7981 aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac ttaacggcgg
     8041 acaaaatgcc gattacattc cctttctggc gaatgtacca ggtcaactgg cggcagtggc
     8101 tatcgtgacc tgcgatggca acgtctatag tgcgggtgac agtgattacc gctttgcact
     8161 ggaatccatc tctaaagtct gtacgttagc ccttgcgtta gaagatgtcg gcccgcaggc
     8221 ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag ttatcgcctt
     8281 agagttgcat ggcggcaaac cgctgtcgcc actggtaaat gctggcgcta ttgccaccac
     8341 cagcctgatt aacgctgaaa atgttgaaca acgctggcag cgaattttac atatccaaca
     8401 gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg aacaaacaac
     8461 caacttccat aaccgggcca ttgcctggct gctgtactcc gccggatatc tctattgtga
     8521 tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct acgctcatca atactgttga
     8581 actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgttga cgcataaacg
     8641 cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg aagggctgta
     8701 tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg gtgtaggtgg
     8761 cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac caccgctgga
     8821 cgaagaaggc aacagtgttc gcggtcaaaa aatggtggca tcggtcgcta agcaactcgg
     8881 ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg taacaaaccc
     8941 ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggc ggggatcttc
     9001 gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctgggtcgc ctttgctttt
     9061 ggcggtattg tggcgatgtt ttccggttat gcctatgcgc gtctgggggc gagctatccc
     9121 agcaatggcg gtattatcga cttttttcgt cgcggattag gcaacggcgt cttttcgcta
     9181 gcgctctcgt tgctgtacct gttgacgctg gcggtgagca tcgccatggt tgcccgtgct
     9241 tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca ccttattttg
     9301 ctctacgcgt tggggatcat tgcggtgatg acgcttttca actccttaag caaccatgcg
     9361 gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt attgctgatt
     9421 attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc ccccagctcc
     9481 ggtgcgttct tctcctgtat tgggataacc ttccttgcct atgcgggctt tggcatgatg
     9541 gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc gtttctggtg
     9601 gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct tagcgatgta
     9661 tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc ttctccgctg
     9721 ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac ggcttcagcc
     9781 attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag cgaacgcgaa
     9841 ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa cattattgtc
     9901 gtggtgttga ttatgctgat gacggcggcg ctaaatttag gctcactcgc cagtgttgcc
     9961 agcgccacct ttttgatttg ctacctggcg gtgtttgtgg tggcgatccg cctgcgtcat
    10021 gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt gttggtgatc
    10081 gttggcttta tctacagtct gtggtcccag ggtagccgtg cgttgatatg gattattggc
    10141 tcactcttac tcagccttat tgtggcaatg gtcatgaagc gcaataaaac cgtataacat
    10201 ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg cactggcaaa
    10261 aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg ttgcgcgaac
    10321 gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca ttcgcttcta
    10381 tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc gcacctacac
    10441 gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg gctttaacct
    10501 ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca gcgccgacgt
    10561 caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg agctgcaatc
    10621 catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca gcgccgactg
    10681 cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggtgat taagacgaga
    10741 cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatgc gtacctgcac
    10801 cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata tgaccgcgcc
    10861 cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc ccgcgctggt
    10921 ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat ttccaccaca
    10981 gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc caacccagcg
    11041 gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac aacaaataac
    11101 catttccgcc cagcatctcg gctgccagca gcaaaccgcc gagactgagc cagaaaatat
    11161 gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc tgtctttcac
    11221 cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg gcatcatcac
    11281 tactttgctg ttactggagg taccgatctg ctgtaacgct tcggtgtatt tctgcgctac
    11341 gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca ttttggtggc
    11401 gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg ccgactgacg
    11461 ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga ggatttccgc
    11521 ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg ctttcatctg
    11581 cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt caatacgggt
    11641 gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc gtgagttgat
    11701 gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg tacggatgtt
    11761 agtcatggtc aggttgatga tcgccagctc cagattgctg acttcataag ccgcgcgcgg
    11821 cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt tatctttcga
    11881 gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct tgcgaccaat
    11941 gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg tatagcgacc
    12001 aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg cgccgacaat
    12061 gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa cctcctgttg
    12121 taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg attactgaaa
    12181 gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc agaaaaaggg
    12241 aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat atctggcggg
    12301 tgatgcgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat ttaagttaat
    12361 taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt cattgatcag
    12421 cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa agccagaaat
    12481 ctaccgccaa caagtctctt actgcgccca gacaccgacg ctgtttggcg atacggtata
    12541 cgataatctg atctttccct ggcagatccg taaccggcag cctgacccag ccatttttct
    12601 cgattttctc gaacgcttcg ccttgccgga cagcattttg acgaaaaata tcgccgagct
    12661 atctggtggt gaaaaacaac gcatctcact gattcgtaac ctgcaattta tgccgaaggt
    12721 tttattgctg gatgaaatca ccagtgcgct ggatgaaagt aataaacata acgttaatga
    12781 gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga cacacgataa
    12841 agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg gagaaatgca
    12901 ggaagcacgc tatgaactcg cataatatta ctaacgaatc attagcactg gcattaatgc
    12961 tggtggtggt ggcaatctta attagccata aagaaaaact ggcgctggag aaagatattc
    13021 tctggagcgt cggacgagcg ataattcagc tgattattgt cggctatgtg ctgaagtata
    13081 ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc tgctttaatg
    13141 cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc tcatcgttta
    13201 ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctca gggtcgattg
    13261 aatttatccc gatgcaggtg atccctatcg ccgggatgat tgccggtaac gccatggtag
    13321 cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag caacagattc
    13381 aggagaaact gagtcttggt gcgacgccga agcaggcttc agcgatattg attcgcgaca
    13441 gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc ttagtgagtt
    13501 taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag gcgattaaat
    13561 atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc attattgcct
    13621 gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg acgcaattga
    13681 agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg ttcatgtgcc
    13741 gatcaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc gacgccagtg
    13801 catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc tgaccgtctg
    13861 cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct tcattgcgcc
    13921 caacctgatg cagttgcagc gccagttgcg tcgccagtgc ggcatcttct ggattctccg
    13981 ccacctgctg ttgcaactgt tgaatttccg gcgtatcagc cgcctgcttc agcagttcga
    14041 tttgcgccac catcccctgg tagcgggtgt cctgatcctg taacggaatg gttttcagca
    14101 ccgcttctgc atcttcagaa cggttcagcg caatcagcgt ttctgccaac agcaggccga
    14161 tctccccgtt ctgattcgac aactgccagg cgtctttcag caatggcagg gcatcggtga
    14221 aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct tcgcgcggca
    14281 gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg aagccatcta
    14341 ccggttgccc gttctggaac agatacaccg tcggaatcgc gcgcagacca aactgggcgg
    14401 caatcatctg ctccgcatcg cagtccagct tcgccagaat aaattgcccg ttgtactgcg
    14461 ccgcaaggct ttccagaatt ggggttaact gcaaacagtg ctggctacgt tcagaccaaa
    14521 aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg ttagattcgt
    14581 taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat attttctttg
    14641 acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc atcacgcgcc
    14701 ccggcagcag gcgcttaagc accattactg cccaagtcac cagcgttacc ggatagcgca
    14761 tcttcggctt ctcgctaata aaagcatggc gtactttgtc caccaccgct tccggtccca
    14821 acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc gtctggttga
    14881 cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact ttaattccgc
    14941 tgtggcgtag ctccatgcgc agcgcgtctg accatgcctc cagcgcatat ttactggccg
    15001 cgtaagcgcc acgacccggt gtggagatta atcccatcac cgatgatgtc atcacaatac
    15061 gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg tgtgcgccga
    15121 aaaagttggc ggaaaactgc tgttccatct gcgcgcggct gatggtggaa agggggccat
    15181 acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc agggcgatca
    15241 cttcgtcggc tgcgcgatca acactttctg gcgaatccag atcgatcaac acgccggtaa
    15301 atcccatgct gttcatacgc tcaacatcat ccggtttccg gcaacctgcc agcacatgaa
    15361 aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa catccggtaa
    15421 ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta tgagtcatga
    15481 tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg ggcgtcgcgg
    15541 ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc ttccataaaa
    15601 aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc gctaaaggct
    15661 tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa tggttcagcg
    15721 ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg ctgtggctga
    15781 aaaccacgca aaccgtcatt gccgcccagt tcaaccagca cccaacgcgg ctgatgctgt
    15841 ttcagcagag ccggaaggcg cgccagtcct tgttgcgagg tgtcgccgct gatgctggca
    15901 ttaactaccg acgttttact ctgccactta tcattcaaca aggcaggcca ggccgcgctg
    15961 gcagacattc gatacccggc gctcaggcta tcacccagaa tcaataacgt gtccgctgcg
    16021 gcggcacgga aggttaacag ggccaggaac aggaagggca aatgccagcg gaaaacattg
    16081 ttgaagttca tcatcttaag aagtccgtcg gtcaggggga gcatgaactc tccatcctca
    16141 ccggagttga gctggttgtc aaacgtggcg agaccatcgc actggtgggc gagtcgggat
    16201 cgggtaagtc aaccttgctg gcgatcctcg ccgggcttga tgacggcagc agtggcgaag
    16261 tgagtctggt gggacaaccg ctacataata tggacgaaga agcgcgggca aagttgcgcg
    16321 cgaagcacgt cggctttgtt tttcagtcat ttatgttaat tcctaccctt aacgcgctgg
    16381 aaaacgtcga gcttccggct ctgctgcgcg gtgagagtag cgcggaaagt cgtaacgggg
    16441 cgaaagcgtt gctcgaacag ttagggctgg gtaaacgtct ggatcatctt ccggcacagc
    16501 tttccggcgg tgaacagcaa cgagtggcgc tggcacgagc ctttaatggt cgacctgatg
    16561 tgctgtttgc cgacgaaccc accggcaacc ttgaccgcca gacgggcgat aaaattgccg
    16621 acctgctgtt ttccctcaac cgtgaacatg gcaccacgtt gattatggtg acccacgacc
    16681 tgcaactggc ggcacgctgc gaccgctgct tacggctggt gaacgggcag ttgcaggagg
    16741 aagcatgatt gcacgttggt tctggcgcga atggcgttcg ccgtcgctat taattgtctg
    16801 gctggcgcta agcctggcgg tggcctgcgt gctggcgctg ggcaatatca gcgatcgcat
    16861 ggagaagggc ttaagccagc aaagccgtga gtttatggcg ggcgatcggg cgttgcgcag
    16921 ttcacgcgaa gtgccgcaag cgtggctgga ggaagcgcaa aagcgcggcc tgaaagtcgg
    16981 caagcagctg actttcgcca caatgacctt tgcaggcgac acaccgcagc tggcgaacgt
    17041 caaagcggtg gatgatatct acccgatgta tggcgatctg caaactaatc cccctggcct
    17101 gaaaccgcag gcgggcagcg tattgctggc cccacgcctg atggcactgc ttaacctgaa
    17161 aacgggcgac accattgacg tgggcgatgc caccttgcgg attgccggag aagtgattca
    17221 ggaaccggat tccggtttta accccttcca gatggctccg cgtctgatga tgaatctggc
    17281 ggatgtcgat aaaaccggag ccgtgcagcc ggggagtcgg gtcacctggc gttataaatt
    17341 cggcggcaac gagaaccagc tcgacggcta tgataaatgg ttgttacctc agcttaaacc
    17401 cgaacaacgc tggtacggtc tggaacagga cgaaggcgcg ctggggcgat cgatggaacg
    17461 ctcgcaacag ttcctgctgc tttcggcgct tctgaccttg ctgctggcag tggcagcggt
    17521 ggcggtagcg atgaatcatt actgtcgcag tcgctacgat ctggtggcga tcctcaaaac
    17581 gctgggggca gggcgagcgc aactgcgtaa gctaatcgtc ggtcagtggt tgatggtgct
    17641 gacgctttca gccgttaccg gtggggccat aggcctgttg ttcgaaaacg tgttgatggt
    17701 gctgctcaag cccgttctgc ctgctgcact accgccagcc agcctctggc cgtggctgtg
    17761 ggcgcttggc accatgacgg tcatctcgct gctggtgggg ctacgaccat accgcttgtt
    17821 gctggcaacg cagcctttac gcgtattacg taatgatgtg gtagcgaacg tctggccgct
    17881 gaagttttat ctgccgattg tcagtgtggt ggttgtgctg ctgctcgccg gattaatggg
    17941 tggcagcatg ctgctttggg cggtgctggc gggcgcggta gtactggctt tgctgtgcgg
    18001 tgtgctgggc tggatgcagc tgaatgtact tcgccgcatg acgctgaaat cgctacctct
    18061 gcgcctggcg gttagccgcc tgttacgtca gccgtggtca acgttaagcc agctttcggc
    18121 attttcgctc tcctttatgc tgctggcgct gttgctggta ttgcgtggcg atctgctcga
    18181 ccgctggcag cagcagctac cgccagaaag cccgaactac tttttaatta acatcgccac
    18241 agaacaggta gcaccgctaa aagcgttcct cgcggaacat catatagtcc cggaatcatt
    18301 ttatccggta gtgcgggcgc ggctgacggc gattaacgat aagccgacag aaggtaatga
    18361 agatgaggcg cttaatcgcg aactcaatct tacctggcaa aatacgcggc ccgatcataa
    18421 tccgattgtc gccggtaact ggccgccaaa agccgatgaa gtgtcgatgg aagaggggct
    18481 ggcgaaacgc ttaaacgttg ccctcggtga taccgtgact tttatgggcg atacccagga
    18541 gttccgcgct aaagtgacca gcctgcgcaa agtggactgg gaaagtctgc ggcctaattt
    18601 ctattttatt ttcccggaag gggcattaga cgggcaaccg cagagctggc ttaccagttt
    18661 ccgctgggag aatggcaacg gcatgttgac gcaactcaac cgccagttcc cgaccattag
    18721 cctgttagat attggcgcga ttttaaaaca ggtcggtcag gtgctggagc aggtaagtcg
    18781 ggcgctggag gtgatggtgg tgctggtcac cgcctgcggt atgttgctgt tgctggcaca
    18841 ggtgcaggtg ggaatgcgtc agcgtcatca ggagctggtg gtgtggcgca cactcggtgc
    18901 ggggaaaaaa ctgctgcgta ccacgttgtg gtgtgagttc gccatgcttg ggtttgtttc
    18961 tggcctggta gccgcaattg gtgcggaaac ggcgctggca gtgttgcagg cgaaagtgtt
    19021 tgatttcccg tgggagccag actggcgatt gtggattgtt ctgccgtgca gcggtgcact
    19081 gctgctgtcg cttttcggcg gctggctggg tgcgcgactg gttaagggta aggcgctgtt
    19141 caggcagttt gcggggtgat gacaggtgtt agattgcctg atgcgctacg cttatcaggc
    19201 ctacgaagag cgtgcaatat attgattttg caagattgtg taggccggat aaggcgttta
    19261 cgccgcatct ggcatgaaca gtgtgcattt tggcaataaa tatgaaacca actgtcatgt
    19321 cggtttttta tatctgtatt ctggtgcaac cattctgttc tcacgacaat cgccgtttca
    19381 gaataccgcc catcactgcg tcttaaccca ttccgccact tccgcccact caccgcgaaa
    19441 gacaactttt tccgcttttt tctcaagctg atagcgatac atcgggtcgt aatattcttt
    19501 aagtaacggc accagccagg ccagatgacc gtcggtgctg tcggtggtga gttgcgttgt
    19561 cagcgccgta tccagctggg cagcgagttc gttatagcgc tgtaacccca gccgacgctt
    19621 aatagccgaa agtccgtgat gcaggtattc gcaatattcc tgccagccct gttcgtcgcc
    19681 gtacgcgtgg gtaaaatcat gatgcatacg caagaaatac tcttcgttca ggcgctcaag
    19741 acggatctca aacggatctt ctaccaccgc aatcgccgcc tgagtcattc gctcgcgcag
    19801 gcattccggc aggtgattcg aaccgatcat ccggctttcg tcttccagca cccacaggcg
    19861 caaatcctga cgggcgtcgg tttttagcat ttcggcagcc agcaggtttt caaaactcgc
    19921 ctggctaagt tgtggttgta aggtgcgacc aaacgccgaa ccgcgatgac gcgctaaccc
    19981 ttccagatca acaccgttcg gctgttgctg cactaacagc gttttaccgc tgccggtaca
    20041 accgccaatc agcactatcg gtttttgtgc cagttcaata gtcgcctgaa tcgcggcttg
    20101 gcgcagtgcc ttataaccgc cttccaccag cggataatca atccccgctg catacaacca
    20161 gctttgcaca atatgtgagc gctgaccgcc acgggcgcag cagagaatac cttgcggatt
    20221 ttgcaggcac gctgcccgcc aggcgtccat gcgctgctga cgaatttcac ccgccaccag
    20281 tttatgcccc agcgccagcg ccgcgtctga gccttgctgt ttataacaag tgccaacggc
    20341 ggcgcgttca tcgttattca ttaacggcag gttgatagcg gcgggcattg cgccttgctc
    20401 aaactcgata ggggcgcgaa catcaataat gggcgtatca gcaatcagca gggcacgata
    20461 gtcctgttcc gtgtgtctct cttgcatagt taaaagtgaa cctcaaatca gcttgcgcgc
    20521 tattttacgc gccaacgcgc aaggaaactt gatttttaac tgcgtgggtt gccgaaaatt
    20581 tctaaaaatc cgctgatttc cggcctgcgc tgggtaaaca aggtcacaat atcttctacc
    20641 gctttgccgc tgccaaattt gcgccatgcc agactcaatg gcgaaggagg gcgcatcgtt
    20701 gggattaccc ggctgaccag ttgttgatta tcgatcattg actggcaaag tgattttggc
    20761 aaaaaaccaa tgccaacacc cgccagatgg gcggcgattt tcgtttccat atcgggaaca
    20821 ataatctctt tttgccccgg caatcgccag gcgacgcgtt tggttaaggt tcgggcgctg
    20881 tcttcaatat tgaccgccgg aaagcgccgc aactgcgctt ctgttagcgg ctcttcaacg
    20941 ttcgccagcg gatgatccgc cgccatgaca aagcgccatt gcaccgatcc taagggatca
    21001 agactaaagg tatttgccag cgcctcagtt cccgtgaccc cgatagccag cgaaaaacct
    21061 tcgtacaata gcgagtccca gacgcccata tagatttgtc gggagatgtg aaactgggta
    21121 aaggggtaac gctcattcag ccacgccagc aactgggcga cggcctgggg gttatagagc
    21181 aggttgttga tgacaatatt cacctagcgt tccacgccat cattcacctg ttgcagctcg
    21241 ctgggcatac tttccagcca gctcagccag tctctggcct gggaaagtag atgctcgcca
    21301 gccgctgtca acgtcacgct gcgagtcgtg cggaaaaaca gcgccactcc ggtattctct
    21361 tccagaagtt taatgcgata actgatcgtc gccgtggttt tacataatcg ttctgccgct
    21421 tttgaaaaac ttcctgtttc agcaaccgca atgaaagtcc gcaaggtttc tggatcgaac
    21481 atcttcaggt atcccctttt aaatccgcaa gttgcgtgat tttcttatcc tctgatttat
    21541 cagtattttt acatgataac cctgttcaat ttgtggacta aatctagttt tggaaaaata
    21601 ttccaacttt tgtattgatg ttgttctctt aaggttttag at
//
LOCUS       DAZPLI010000060        21500 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZPLI010000060 DAZPLI010000000
VERSION     DAZPLI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21500
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.060"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            147..1844
                     /gene="ypdA"
                     /locus_tag="ACHUU3_002172"
     CDS             147..1844
                     /gene="ypdA"
                     /locus_tag="ACHUU3_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFR2082236.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1859..2593
                     /gene="ypdB"
                     /locus_tag="ACHUU3_002173"
     CDS             1859..2593
                     /gene="ypdB"
                     /locus_tag="ACHUU3_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR2082237.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2606..3463
                     /locus_tag="ACHUU3_002174"
     CDS             2606..3463
                     /locus_tag="ACHUU3_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR2082238.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(3466..5961)
                     /gene="ptsP"
                     /locus_tag="ACHUU3_002175"
                     /pseudo
     CDS             complement(3466..5961)
                     /gene="ptsP"
                     /locus_tag="ACHUU3_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5986..7023)
                     /gene="ypdE"
                     /locus_tag="ACHUU3_002176"
     CDS             complement(5986..7023)
                     /gene="ypdE"
                     /locus_tag="ACHUU3_002176"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR2082239.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(7023..8108)
                     /gene="ypdF"
                     /locus_tag="ACHUU3_002177"
                     /pseudo
     CDS             complement(7023..8108)
                     /gene="ypdF"
                     /locus_tag="ACHUU3_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(8123..9370)
                     /locus_tag="ACHUU3_002178"
     CDS             complement(8123..9370)
                     /locus_tag="ACHUU3_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFR2082240.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMLGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9392..9718)
                     /locus_tag="ACHUU3_002179"
     CDS             complement(9392..9718)
                     /locus_tag="ACHUU3_002179"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR2082241.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9938..10903)
                     /gene="glk"
                     /locus_tag="ACHUU3_002180"
     CDS             complement(9938..10903)
                     /gene="glk"
                     /locus_tag="ACHUU3_002180"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR2082242.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            11107..12363
                     /locus_tag="ACHUU3_002181"
     CDS             11107..12363
                     /locus_tag="ACHUU3_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR2082243.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12478..12804
                     /gene="ypeC"
                     /locus_tag="ACHUU3_002182"
     CDS             12478..12804
                     /gene="ypeC"
                     /locus_tag="ACHUU3_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR2082244.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12945..14183)
                     /locus_tag="ACHUU3_002183"
     CDS             complement(12945..14183)
                     /locus_tag="ACHUU3_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR2082245.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14519..15721
                     /gene="nupC"
                     /locus_tag="ACHUU3_002184"
     CDS             14519..15721
                     /gene="nupC"
                     /locus_tag="ACHUU3_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR2082246.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15771..17959)
                     /locus_tag="ACHUU3_002185"
                     /pseudo
     CDS             complement(15771..17959)
                     /locus_tag="ACHUU3_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            complement(18168..18243)
                     /locus_tag="ACHUU3_002186"
     tRNA            complement(18168..18243)
                     /locus_tag="ACHUU3_002186"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18208..18210),aa:Ala,seq:ggc)
     gene            complement(18283..18358)
                     /locus_tag="ACHUU3_002187"
     tRNA            complement(18283..18358)
                     /locus_tag="ACHUU3_002187"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18323..18325),aa:Ala,seq:ggc)
     gene            18579..18938
                     /locus_tag="ACHUU3_002188"
     CDS             18579..18938
                     /locus_tag="ACHUU3_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR2082247.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18940..19332
                     /locus_tag="ACHUU3_002189"
     CDS             18940..19332
                     /locus_tag="ACHUU3_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR2082248.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(19384..20799)
                     /gene="gltX"
                     /locus_tag="ACHUU3_002190"
     CDS             complement(19384..20799)
                     /gene="gltX"
                     /locus_tag="ACHUU3_002190"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR2082249.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            21058..21133
                     /locus_tag="ACHUU3_002191"
     tRNA            21058..21133
                     /locus_tag="ACHUU3_002191"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21091..21093,aa:Val,seq:tac)
     gene            21178..21253
                     /locus_tag="ACHUU3_002192"
     tRNA            21178..21253
                     /locus_tag="ACHUU3_002192"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21211..21213,aa:Val,seq:tac)
     gene            21300..21375
                     /locus_tag="ACHUU3_002193"
     tRNA            21300..21375
                     /locus_tag="ACHUU3_002193"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21333..21335,aa:Val,seq:tac)
     gene            21380..21455
                     /locus_tag="ACHUU3_002194"
     tRNA            21380..21455
                     /locus_tag="ACHUU3_002194"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21413..21415,aa:Lys,seq:ttt)
BASE COUNT         5307 a         5724 c         5342 g         5127 t
ORIGIN      
        1 agtgcattat caagagatac attgctaata caccatttat ctttcaatcc ccttttaaat
       61 ttatggtttt tcctcaaaaa cggcctgctg gtcatcagcc ttcaggttac ctatcataga
      121 ggtttaatcc ttattcagag tcacccgtgc acgaaatatt caacatgctg ctggcggtct
      181 tcgatcgggc agcgttaatg cttatctgcc tgttctttct catccgtatc cgcctgtttc
      241 gcgaactgtt gcacaagtcg gcgcactccc caaaagaatt gctcgccgtt accgccattt
      301 tctcgctgtt cgccctgttc agcacctggt ccggcgttcc cgtagaaggc tcgctggtga
      361 acgtacgtat tatcgcggtg atgtccgacg ggattctgtt tggcccgtgg gtaggcatca
      421 ttaccggcgt gattgcgggt attcaccggt atttaattga tatcggcggc gtgacggcga
      481 tcccctgctt tatcaccagc attctggcgg gttgtatatc aggctggata aacctgaaaa
      541 tccccaaagc acagcgctgg cgcgtcggta ttctcggcgg catgttgtgt gagacgttga
      601 ccatgattct ggtaattgtc tgggcaccaa ctaccgcgtt ggggatcgat atcgtctcta
      661 aaatcggcat tccaatgatc ctcggtagcg tctgtatcgg ctttattgtg cttctggtgc
      721 aaagcgttga gggcgaaaaa gaggccagcg ccgcgcggca ggccaagctg gcgctggata
      781 tcgccaacaa aacgctaccg ctgtttcgcc atgtcaatag cgagtcatta cgcaaggtct
      841 gcgaaattat ccgcgatgac attcacgccg atgcggtggc gattactaat accgatcatg
      901 tgctggccta tgttggcgtg ggtgaacata actatcagaa tggcgatgac ttcattagcc
      961 cgactacccg tcaggcgatg aattacggaa aaatcatcat taaaaacaat gatgaagccc
     1021 accgcacacc agagattcat tccatgctgg tgatcccatt gtgggagaaa ggggtcgtga
     1081 ccggaacgct gaaaatttac tactgccacg cgcatcagat cacctcgtca ttacaggaaa
     1141 tggcggtcgg tctgtcgcaa atcatctcca cgcaactgga ggtttcacgc gccgagcagc
     1201 tacgtgaaat ggcaaataag gcagagcttc gcgccctgca aagcaaaatt aatccccatt
     1261 ttctgtttaa cgctctgaac gctatttcat cgtcaatccg tctgaatccg gataccgctc
     1321 gccagttgat ctttaatctg tcgcgttatc tgcgctataa cattgaatta aaagacgatg
     1381 agcaaatcga tatcaaaaaa gagctgtatc aaattaaaga ctatattgcc attgagcagg
     1441 cccgctttgg tgacaagctg acggttatct atgatattga tgaagaggtg aattgctgca
     1501 ttcccagcct gctgatccag ccgttggtgg agaacgccat tgtccacggt attcagcctt
     1561 gcaaaggtaa aggcgttgtc accatcagcg ttgcagagtg cggaaatcgg gtacgcattg
     1621 cggtgcgaga taccgggcac ggcatcgatc caaaggtgat tgagcgggtc gaagcgaatg
     1681 aaatgccggg caataaaatt ggcctgctga atgtccatca tcgcgtgaag ttattgtatg
     1741 gcgaggggct gcatatccgc cgcctggagc cggggacgga aattgcgttt tacattccta
     1801 accaacgcac cccagtcgcc tcacaggcta cgttattgct ttgagccgga gtgatattgt
     1861 gaaagtcatc attgttgaag acgaattcct ggcacaacag gaactgagct ggctaattaa
     1921 agagcacagc cagatggaga ttgtcggcac ctttgacgac ggtctggacg tgttgaagtt
     1981 tttgcagcat aaccgcgtcg acgccatttt tctggatatc aatattccgt cgctggatgg
     2041 cgtgttgctg gcgcaaaaca tcagccagtt cgcccataaa ccgtttattg tgttcatcac
     2101 cgcgtggaaa gaacatgcgg tagaagcgtt tgaactggag gcgtttgact acattctcaa
     2161 accgtatcag gagtcacgta ttaccgggat gctgcaaaaa ctggaagcgg cctggcaaca
     2221 acagcagacc agcagtacgc ctgccgcgac ggtaacgcgt gagaatgaca ccattaatct
     2281 ggtgaaagat gagcgaataa tcgtcacgcc aattaacgat atctattacg ccgaagcgca
     2341 cgagaaaatg acctttgtct atacgcggcg tgaatcctac gtaatgccga tgaacattac
     2401 cgaattttgc agcaaactgc cgccgtcgca ttttttccgc tgccatcgct cattttgtgt
     2461 caatctgaac aaaatacgcg aaatcgaacc gtggtttaat aacacctaca ttctgcgact
     2521 gaaagatctg gattttgaag tgccggtcag ccgcagcaaa gtgaaagaat ttcgccagtt
     2581 aatgcatctt taatcgggaa ctttcatgaa agcaccaggc ttgcccgccg atcaacaatt
     2641 tttcgccgat ctgttcagcg gcctggtgct taacccgcaa ctactggggc gcgtctggtt
     2701 tgccagccag cctgcctcgt taccggtggg cagtttatgt attgattttc cccgtctgga
     2761 tatcgtgctg cgcggcgaat acggcaacct gctggaagca aagcagcagc gaatggtgga
     2821 aggagaaatg ctgtttattc cggcacgcgc ggctaattta ccgatcaaca acaaaccggt
     2881 gatgctgtta agcctggtgt tcgcaccgac ctggcttggg ttatcgtttt acgatagccg
     2941 caccacgtcg ttgttgcatc ctgctcgcca gatccagctt cccagcctgc aacgcggtga
     3001 aggtgaagcg atgcttaccg ccctcaccca tcttagccgt tcgccgctgg agcagaatat
     3061 cattcagcca ctggtgttaa gtttgctgca tctttgccgc aacgtggtga atatgccgcc
     3121 gggtaattcg caaccgcgcg gcgattttct ctatcacagc atttgtaact gggttcagga
     3181 taattatgcc cagccgctca cccgcgagag cgtggcgcag ttttttaata tcacgcccaa
     3241 tcatctgtca aaactgtttg ctcagcatgg aacgatgcgt tttatcgagt atgtgcgttg
     3301 ggtgcgaatg gcgaaggcga ggatgatttt gcagaaatat catctgtcaa ttcatgaagt
     3361 ggcacagcgt tgcggttttc cggatagcga ctatttttgt cgcgttttcc ggcgtcagtt
     3421 tggtctgacg ccgggagagt acagcgcccg ttttcagggc taacgtcaga aggttaattc
     3481 tgtttccagc agcgtcagga tactttgtgc atcttgcgcg gcaaacaacg actggcggaa
     3541 gtttttattc accagtttac gcgccagctg cgagaagact ttcacatgat tcatcccttc
     3601 gttagcaccc agcgtcagca tgatcaccag ttcaacttcg cccatttctg actgccagtc
     3661 aaccggtttc gccagccggg caatgctgat actggaatga cggatccact gagatttggt
     3721 gtgcgggatc gctacgccaa aaccaacgcc ggtggtaaca atctcttccc gctgccagac
     3781 atcttcttcc agctcaaacg gatgttcagt gcgcccgtta acgccgaggt tgccgcacag
     3841 gaactggatc gcctgctctt tattgctaaa atcctgatca acaaagatat tctccagcgc
     3901 cagcagtggg cgaacgtctt cttccggcgt aaaggcggtg agtaacgctt caatttcctg
     3961 cgcactgcgg cattcacatg cctgacgcgc cagttcccga cacgcctcgc tatccagttg
     4021 acgaagctgg cttttcaccg ccggaatacg cgggctactc atactcagct catccaggcc
     4081 cagcccaagc agtagcggca gataacggct ttcaccgccc agttcaccgc aaatgcctac
     4141 ccatttgccc cgctgatgcg cagtggtaac tatttgctgc aacatgcgca ggaacgatgg
     4201 cgtaatcggg ttatatagcg gcgatacgcg cgggttatta cgatcgaccg catacagata
     4261 ctgggtcata tcgttggagc cgatactgaa gaaatcgacc tcatcggaga agtggtcgat
     4321 gatgtagcac accgacggca cttccaccat gatcccaagc gtaatcgtct ctgcatgacg
     4381 caggccatcg cgcttaagct caacgatcgc tttttggatc tcgcctttca cccataagat
     4441 ctgatcgagg ctgtgaacca tcgggatcat caactgggcg ttgccgaaac tggcggcgcg
     4501 caaaatggcc cgcagttgag tgcggaacag gccagcaaat tccgggtaaa tacgtaccgc
     4561 gcgatagccg aggaacgggt tctcttcctg gggaatattc agataaggaa tgcttttatc
     4621 gccgccgatg tccatcgtgc ggaagataat cggcttgtcg cccgccgcca gtagcacctg
     4681 ctggtaggct tcaaattgct cctgctcgtc cggcgcgctg tcgcgatcca tatagagcat
     4741 ttcggtacgg aacagcccga caccttccgc gccgttggca aacgcgcctg gcgcttccag
     4801 agcggtgccg atattcgccg caatatcaat acgcttgtta tcacgggaat aggccagctg
     4861 cgcggcagcc tgcgcctgtt gtttttggcg tttatccgcc agcgtctgcg cgacctgata
     4921 ataaccgctc accgcgtcat tcgggttaat cgccagcacg ccgcactggg cgtcaagcac
     4981 tgcaggttgc ccggcataac gggcaatcgc atccagcggc aagccactca gtaccgggat
     5041 cgccgaggca cgggccagaa tcagtgtatg cgaggtgcgc ccggttttct ccagaatcat
     5101 gcccgcaaga tttttcaaat caaggctcaa aaactggctt ggggttaaat cttcagccac
     5161 cagaatggtc ggtttttcca gcaccagatt gttgcgcggc ttcagttccg gctaggtgat
     5221 atgcagcaac tgttcgctga tatcgcgaat gtcgctgacc cgctcgcgca gataatcgct
     5281 ggcagaagca gatagtttgg cgcaaacctg ctccatattg ctgatgatcg ccgcccccag
     5341 tccctgatgc tgttctgtca tcaggcgacg gatattgcct gcaaattcat catcctgaat
     5401 cagcgacaaa tgagcgctga ggatagtttt gctttcgccg tcacgctcac gcaattgctg
     5461 gttcagttgc tcggcaagcg ttgccaggct gtgctccagc cgggtggaat cttgcgcact
     5521 ggcggggatt gcccgataac tgtcgaggct gtcgctttgt aacagggtca gcgtacccac
     5581 gccgacgccg cttgccagca cattgccgta cagtaaatcc gggttaaggc ggcttaatga
     5641 acgcggcagc ggatgcgccg tcagttctgc ctgcgtcggc tgaacgctgt cgctgtcgat
     5701 aaagcgcacc aggatgtact cttccagcac gcgccgcgcc tgctcttcat cgctgccgct
     5761 aatgttcagg ctgcaactgt cattaaataa ggtgccagtt ccaatcaacg ccagcgagct
     5821 tttagcatcg gcctttgcgt tctggcgatg gttgataaag gtgatttcgc tttgccactg
     5881 gctgcactgt tctttaagtt cccacgccgg acgagcatgt agaccgttag gcaaaggaca
     5941 gagaaattga atcgttaaca taaccgctcc gggttaatca ggagatcatc tgaaatccgt
     6001 cagttgaaca accgtctcac gcgtaagacg ttgaataagg gcagataaaa gttgctccat
     6061 ctgcaaaatg tcgcggcaat cggcaatcga tgccgcgcaa tgtccgtggc gggtggcagg
     6121 ccccattacc actgtgggta cgccagtatc ggttaagtgc accgctccac cgtccgtgcc
     6181 gccgttactg aacatatccg cctgcaacgg aacgccaatt tctgccgaca cggtttcaat
     6241 ccacgcggta agttttggcg gcgcaatcag cgatttatcg cttaacacca gcatcggccc
     6301 gttaccaatc tgacgatggt tagccgcgcc ataatcaaag tttttcgccc agcaggcggt
     6361 atcaagcaca atggcgacgt ccggcgacac cgcgcgggta gcagtttgcc cgccgcgtaa
     6421 tcccacctct tcgctggaac ttgccaccag ccacacttcc gcaggcagtt cggcgtcgtg
     6481 cagctcacgt agtaacgtca ccagcagata gcaaccgagg cggtcatcaa aggctttccc
     6541 catcacccgc tggtgaggga gaacctgaaa agttgtatca aaggtgacgc gatcgcctgg
     6601 acgaattccc gcctgcatca cttcgtcata cgagcgcgca ccaatgtcca cgcgcatggc
     6661 gctgacgtca ttcccctgcc ggtcgccgtc aagcaggcct ggaattttgc actcttcacg
     6721 ggtggtgatg cgcaccggct gcagctggcg ggcagccatg cgcacgttgc caaccggcag
     6781 cacatcaatt gccccttcgc gggagatgct gcgcaccata aatcccactt cgtccatatg
     6841 cgcacagatc atcaccttcg gacctgtcga ttcattgagg cggatcagca ccgatcccag
     6901 accatcaaat cgtacttctt tttgcaggcg atccgcttct tccagtagga tctgccgcac
     6961 ttcctgttcc gaggaggcga tcgcatctgc ctcgctcaac gcttttaata gcgataaatc
     7021 cattatgcct ctcccgtgag caacactgtt ttcggcatgg cgtagagcac ttctgcgcct
     7081 tgcggggtaa ccagcacaac atcttcgatg cgcacgcccc cttgccctgg caaataaatc
     7141 cccggctcca cggtcagtaa catgcctggc tgtagcgtcg tggtatcccg cggtgaaaag
     7201 cgtggatctt cgtgtacttc aataccgata gcatgaccgg tgttgtgacc aaaatagtcg
     7261 ccaaatcctg cctcggtaat cacccggcgc gcggcgtcgt caacctgtta gcagcgtacg
     7321 ccagggcgaa tcgcggagat tgccgccagc tgtgcttgca ggacaatctg atagacgtta
     7381 aacagcgggt gagattcggc aattacccct tcgccattca ccagcaaggt gcgcgtcata
     7441 tcagagcagt acccctgata gagcgcacca aaatcaagag tgacaaactc gcctgctgca
     7501 acaatcttgt cgctggcttt gccgtgcggc agcgccccac gccagccgct ggcgacaatg
     7561 gtgtcaaaag aggctttttc tgcgccctgc tggcgcataa accactccag ttcagcggct
     7621 atctcgcgct cgctcatccc cgcctgaata aagcggcgaa tatgctccgc ttcgcgatcg
     7681 gcaatcccac aggcgaggcg gattatctcc acctcctctg gcgttttgat ttgccgcagc
     7741 acatccggcg tggcgctaac cagtttcgca ttgagttcag acttccagcg gtgcgcggtt
     7801 tcccaactca cctgctggcc ttcaaaaccg agcgtttgca actgctcatc ggcaatgatt
     7861 tgattgacga tagtggtaag cgtgttcgtc gcgtcaagca aatgcagctg gtagccttgc
     7921 gcacgggctt ccacctcaac gaaatagcgc gaatctacca gaatgtgcgc actttcacgg
     7981 ctaataacca catagcctga tccggtggag atccccagat gcggctgttt gttctgccgt
     8041 gaggagagaa gcactgcatc cagttgttgc gccttaagcc agtcgcgcag cgaagcgagt
     8101 aatgtcatct ggattttcct tcttacaggc tatcgattaa caatttacct ttacggaaca
     8161 tcatcagacg caggaacacc accatcagcg cagttatgac tgcccccagc gcgatccccg
     8221 ccatatagac gcccaggtta gtgaccagcg gccatgccca aatagcggat tccgggaacc
     8281 attgcactgc gcccagccag acagcggcgg ttgagccgac aatcgcgccg accatatacg
     8341 acggaatggc ggtgatgggg ctttccagcg caaacggaat cgcaccctca ctaatcccca
     8401 taaaggcaag gaacatcgcg gtcttaccct gcggataaag ttgagcgttg aacaggcgtt
     8461 tgccggttaa acgacggtcg ataatggtcg ccagacccag acctatcggc ggaataacga
     8521 tagcgataga acgtgcagtg accggtaata cgtggtcagt ggtaaagctg aaggcaacaa
     8581 aacctgcggc tttgttgatc gggccaccaa ggtcgattgc tgtcgccgcg gcgatcccca
     8641 ttgcgtacat cagcgcgccc ttctcacctg cggcagtcag cacggtacgg ataccgccgt
     8701 tgatccagcc gccaaagggg gtaatgacgt agtacatcgc cagcatgaca aaaatggcgg
     8761 aaagaatcgg tagcaaaaat gtggttttga acgctaacag gaaatccggt aactggattt
     8821 tctggttcat ccacttcaca aggtaacccg cgacgataga aataatcagc gcaccaatga
     8881 aggtggacgg caccggcgaa gaggtcgccc attgcatagt gctgggatcg aagttcagca
     8941 gttgcgtcgg ctgggtagac atcaagccgc cgataaaacc agccgggaac gccagtttgc
     9001 caccgataga gttcgccaca aaagcagcaa acatcggaat ggcaaaacca aacaacacgc
     9061 cgccgaagga ctgcgacagc caggcaaatt tcagtaagga gaggtcgaaa ccggagaatt
     9121 tcccgctatt aagagcatcc atgatgccga tctcagcagg aattttaagc cagctataag
     9181 caatcagctg gctgaaagcg aggatcacac cgcccataat taaggtcggc accatgcgtg
     9241 aaatccctaa catcacatgc tgcgggagtt caccccagaa gctgctttta gaggcctgcg
     9301 gattcttaac tgccgctgcc gcaccggatg cgccaggcac aacggttgca ctgcgttttt
     9361 taatggccat aacaattatc cctgtcgaat attactgttg ttcagaggca atcatctctt
     9421 cgatttcttt gatgatgccc gcagcgtttt taattgcgtc ctgcaaagtg atttcataaa
     9481 cgtcgcgtga ttcgaaacgc tcattatctt ccggggtaac tgccacggag tggatgatga
     9541 tggttgcttc ggcgatatcc tgcgccgtca ggcgattctg gataccgtcc gcgccctggg
     9601 tttcaatttt cacttcataa cctgcttcta ccgccgcttc ttccagcgcc tgagcggcca
     9661 taaaggtgtg agccaggccc attgggcagg cacataaggc aattagtttc ttactcatcg
     9721 ttaattcctc gttcagtgat agttgacgag tgaaattatg aacgtgcaaa acaaatcgcc
     9781 gttaccagac aaataatgca tttactggag aattaatccc gctgtacgga agtgtgacgc
     9841 aggtcggcct tgtgaggggg acacaaagaa aaattgaatt aactgtatga tttaaaagat
     9901 tatcgggaga gttacctccc gatataacag aaaggattta cagaatgtgc cctaaggtct
     9961 ggcgtaaatg tgcaccggaa ccgagaagac ccggattgtc atggacgatg agatacaccg
    10021 gaatatcatg gacatattct ttaaagcgcc ctttatcttc aaatgcggca cggaaaccag
    10081 aggctttgaa gaactcaagg aagcgcggca cgataccgcc agcaatataa accccaccaa
    10141 aggttcccag cgttaatgcc agattgccgc caaaacggcc cataatgacg caaaacagcg
    10201 acaacgcacg gcggcaatcg gtgcagctgt cagccagcgc gcgttcggta atatcttttg
    10261 gcttgagatt ttctggcagg cggttgtcgg ctttaacaat tgcgcgatac aaattcacca
    10321 gcccaggacc agaaagcacg cgttccgccg aaacatgacc aatttccgca cgcaatattt
    10381 cgaggataat ggcctcttct tcactattcg gcgcaaaatc aacgtgaccg ccttcgcccg
    10441 gcaagcttac ccaacgctta tcgacatgga ccagatgcgc aaccccaagc cctgttccgg
    10501 caccgtaaac cgcaataggt ttaccttcga ccggttctgc gccaccaaac tgaatcagat
    10561 gctctttttt cagcatcggg atcgccatcg atacagcggt aaaatcgtta ataatttcca
    10621 gatggctaaa accgagattc tttttcattt cggcaattga gaacgcccag gtatggttgg
    10681 tcattgccac ccagtcaccg gtaattgggc aagcgatggc aatacagccg tctttcacct
    10741 cgaccttatg ttcttcaaga taaacgcgaa tgaccgcttc gaggctgggg taatcaagcc
    10801 ctgaataggt cttagcctgc gagatttcac cactggcaat atcacacaga gcaagacgtg
    10861 cgttggtgcc gcccacatca ccgactaatg catactttgt cattcttcaa ctgctccgct
    10921 aaagtcaaaa taattctttc tcacactgta aatacctggg ggcataacaa caacgccgga
    10981 aaggcaggct ccctgtaaat atcgatctgg gtcacacatt tactttatcg tttcagcacc
    11041 aattgcagcg atgccttttt gcaagctggg caaactaagt atctgacccc gcataaggaa
    11101 tagaacatgc tccatccgcg agccagaacc atgttgttat tatcgctccc cgccgtggca
    11161 attgggattg cgtccagtct tattctgatt gtggtgatga aaatcgcctc ggtattacag
    11221 aatttgctct ggcaacgact gccgggaact ctggggatag cccaggattc acctatctgg
    11281 atcatcggtg tattaacgct aacgggtatt gcggtggggt tggttatccg tttcagccag
    11341 ggtcatgccg gaccagaccc cgcctgtgaa ccgctgatcg gcgcaccagt tccgccctct
    11401 gcgctaccag gacttatcgt agcattaatt ctcggtcttg ctggcggcgt cagcctgggg
    11461 ccggaacatc cgatcatgac cgtcaatatc gcccttgccg tcgccattgg cgctcgtctg
    11521 ttaccgcgcg tcaaccgaat ggagtggact attttagcct ctgccggaac catcggcgcg
    11581 ctgtttggta cacctgttgc ggcggcgttg atattttcgc aaaccttaaa tggcagtagt
    11641 gaagttccgc tatgggatcg tctttttgcg ccgttaatgg cggcagcagc tggtgcactt
    11701 actaccggat tatttttcca tccccatttt tcactgccca ttgcgcatta cggacagatg
    11761 gaaatgaccg atattctcag cggtgcaatt gtcgcggcga ttgccatcgc agcagggatg
    11821 gtcgccgtat ggtgcttacc acggttgcac gcgatgatga atcaaatgaa aaatccggtg
    11881 ctcgtgctgg gtattggcgg atttattctc ggaattctgg gggttattgg tggaccagtt
    11941 tcgctgttta aagggctgga tgagatgcag cagatggtgg caaatcaggc tttcagcacc
    12001 agcgattact ttttgctggc ggtaattaaa cttgccgccc tggtcgttgc tgccgccagt
    12061 ggctttcgcg gtgggcgaat cttcccggca gtgtttgtcg gcgtggcatt agggttgatg
    12121 ctgcatgagc acgttcccgc cgttccagcg gcaataaccg tttcctgcgc tattctcggc
    12181 atcgtgctgg tggtaacacg cgatggctgg ttaagtcttt ttatggcggc agtcgttgta
    12241 cccaatacca cattgctacc gctgctctgt atcgtcatgc ttccggcatg gctgttatta
    12301 gcaggtaagc cgatgatgat ggtcaatcgt ccgaagcaac agccacccca cgataatgtt
    12361 tagcaaaaaa tgctctcctt tatgatttaa gagttatggc gatgatacgg ggctttacct
    12421 ccccgtaata ttgcgttaac aggccgctga cggcttcgat cgtgaaggag aataacaatg
    12481 ttcaggtcac tgtttctggc ggccgccctg atggcattta ccccgcttgc agcaaatgca
    12541 ggtgaaatca ccctactgcc atcgatcaaa ttacaaattg gcgatcgcga tcattacggt
    12601 aattactggg acggtggtca ctggcgagat cgtgattact ggcatcgcaa ttatgaatgg
    12661 cgcaaaaacc gctggtggcg tcatgataat ggctatcatc gtggctggga taagcgtaaa
    12721 gcatatgagc gtggctatcg tgaaggctgg cgcgatcgtg acgatcatcg cggaaaaggc
    12781 cgcggacatg ggcaccgcca ttaaacgcgt cctccaatgg agcacaatgc ctgatgcgac
    12841 gctagcgcgt cttatcaggc ctacaaaccg gctctttaac gcgtagggcg gataaggcgt
    12901 ttacgccgca tccgccaata gtgccagatg cgacgctcat tcaactacaa tcccagcgcc
    12961 gtccccacca acaaccagat attcagcgcc acgaccagca ctacaatcac ccagcctgtc
    13021 tgttttacgc gtttgctgtt caccagatcg cccatcaact tgctgtcact ggtgaaaatc
    13081 agcagtggaa ccagcgccag ggcgatacca aaacttaaca gcacctgact cataaccaga
    13141 atccgtgtcg gatctaatcc catcagaatg acaataaatg acggcaacat ggtgactgta
    13201 cgacgcaccc acagcgggat atggaagcga atgaatccct gcatcaccac ctgccccgcc
    13261 agtgtcccca ccaccgttga ggacagtccg gcagcaacca gacttaaccc aaagaccgtt
    13321 gccgcagcat ggcttaacag cggttgcagc gtcagataag cctcatcaag atcggcaaca
    13381 ccagtatgac cagaaaagtg gaacgccgcc gcagctgtag ccatcatcgc cagattgaca
    13441 aaaccggcaa tcgtcatggc gatagccaca tcccatttgg tggcggaata acgttgttga
    13501 cgcgaaccgc catgtaaatg ctgagtgagc gaagagtgca aataaatcac atgcggcata
    13561 atcgtcgccc ctaacacgcc tgctgccagg aagaccgcct ccgaagtagg taaactcggg
    13621 atcaccattc ctttacccag ctgcgccagg ttaggctggg agaaaatcaa ctcgacaatg
    13681 taagccgcgg caacaaacaa cagtaacccg ccaatcactt tctccagtgg tttttgcccg
    13741 cgacgttgca gcattaaaat caggaaagtc gcgatccccg tcagcaccgc gccctgcaac
    13801 aacgagacac caagaatgag tttaaaaccg atcgccgcac caataaattc cgccaggtcg
    13861 gttgccatcg caataatttc tgcctgaacc caatagaacc acactacggg acgcggatag
    13921 tgatcacgaa tctgctccgc cagatttttt ccggtggcaa tccctagttt ggcagagagg
    13981 atctgaatca gcatcgccat caggttggcc caaacgacaa cccacagtag ctgatagccg
    14041 aagctggcac ccgcctgaat attggtcgca aagttaccgg gatcgatata accaatcgcc
    14101 gcaatgaacg caggtcccat taatgcgagc ctcatcttgc gcgccgcccg tccgctgcta
    14161 ctctcaacgc gatagttcgt catcttgtgc ctctaaaaca tagcctttgc tatgtttcat
    14221 gctatgataa acgagaatga ttatcaaatt catttaaatg gattgtggtg atttctctga
    14281 tagaccagga ttatgactac gaaaagattg atgacttgaa tgttatggtt atgtttaatg
    14341 ttagcacatt tacataactt tcagcttcca tacacaacat agcagaaatg tatgacagat
    14401 cactattttt gaagcctgtc acaggacgtc attatagtgt gtgtcagatc tcgttttcct
    14461 taaccatgtt acatagaatg tgcacggaaa tttaacctgc ctcatatttg gagcaaatat
    14521 ggaccgcgtc cttcattttg tactggcact tgccgttgtt gcgattctcg cactgctggt
    14581 aagcagcgac cgcaaaaaaa ttcgtatccg ttatgttatt caactgcttg ttatcgaagt
    14641 gttactggcg tggttcttcc tgaactccga cgttggttta ggcttcgtga aaggcttctc
    14701 cgaaatgttc gaaaaactgc tcagatttgc caacgaaggg actaacttcg tctttggtag
    14761 catgaatgat caaggcctgg cattcttctt cctgaaagtg ctgtgcccaa tcgtctttat
    14821 ctctgcactg atcggtattc tccagcacat tcgcgtgttg ccggtgatta tccgcgcaat
    14881 tggtttcctg ctctccaaag tcaacggtat gggcaaactg gaatccttta acgccgtcag
    14941 ctccctgatt ctgggtcagt ctgaaaactt tattgcctat aaagatatcc tcggcaaaat
    15001 ctcccgtaat cgtatgtaca ccatggctgc cacggcaatg tccaccgtgt cgatgtccat
    15061 cgttggtgca tacatgacca tgctggaacc gaaatacgtc gttgctgcgc tggtactgaa
    15121 catgttcagc acctttatcg tgctgtcgct gatcaatcct taccgtgttg atgccagtga
    15181 agaaaacatc cagatgtcca acctgcacga aggtcagagc ttcttcgaaa tgctgggtga
    15241 atacattctg gcaggtttca aagttgccat tatcgttgcc gcgatgctga tcggctttat
    15301 cgccctgatc gccgcgctga acgcactgtt tgccaccgtt actggctggt ttggctacag
    15361 catctccttc cagggcatcc tgggctacat cttctatccg attgcatggg tgatgggtgt
    15421 tccttccagt gaagcactgc aagtgggcag tatcatggcg accaaactgg tttccaacga
    15481 gttcgttgcg atgatggatc tgcagaaaat tgcttccacg ctctctccgc gtgctgaagg
    15541 catcatctct gtgttcctgg tttccttcgc taacttctct tcaatcggga ttatcgcagg
    15601 tgcagttaaa ggcctgaatg aagagcaagg taacgtggtt tctcgcttcg gtctgaaact
    15661 ggtttacggc tctaccctgg tgagtgtgct gtctgcgtca atcgcagcac tggtgctgta
    15721 aaaccataca taaaaaagcc ggggatcatt ccccggcttt tttacgcctg ttaatcagct
    15781 aatggctgcg ggcgaccaat caaataccct tgcagatatt gcaccccgag cttatgcaat
    15841 agcgcctgct gctgttgcgt ctcgacaaac tccgcgacca cactcaatga cttcgctttc
    15901 gccagatcgg taattgatct cacaatcatc gcatccagcg tattcgtgac aatatctttc
    15961 acaaagacgc catcaatttt gatgatatca gcctgcaaac gctttaaccg ttcgtagttg
    16021 gcatatccgg tgccaaagtc atcaatcgca atccggaaac caaacttatg cagctgctcg
    16081 atgttgtaca tgctgctttc tgcgttagaa aacgcctgct cctcggtgat ctcaagaatg
    16141 accgcctgcg gggagatgtg ataacgctta aacagacgaa taatccgccc ggcaatattc
    16201 ttttgcagca gcgtgagcgg cattaaattg actgaaaagc gcggtccttt tttgtcgcaa
    16261 gggtgtgttg ccagccactt caacaaggat tccagcactt gcaaatcaaa acgcgcgcta
    16321 aggttaaact gagcaataag gggcagaaac ttatccgggg tcataatgcc gccgtcatat
    16381 ttcagtcgcg cgaggatctc atcataacct tcaccctctt tgttgcgaat tggctgggcg
    16441 tagagcagca aatcaccctg atctaacgcc gtgcgaatgg tattcagcaa tagcacctgt
    16501 ttagtggtct gcccggaaac catctcctct ctgctatcca gcgccagcac atgatgatgt
    16561 gcgcaggatt gctccgccag ccagcttaac tgccccaaca agggttgcag ggtttcctga
    16621 tttccatcaa aacgccccca ggcagcacca tagcccatat ccagcccggt attgttccag
    16681 tgaatttgcc gactattcag gatgttaacc atatgctgga gtcgcccttc cgtttccggc
    16741 ccgctcagca ctaacagcag ttcactaccc ggcaattgat ataacttttc gttttcctgc
    16801 atcaacggca gcagcgtacg gtaaattgat cggatacagt gaacgcgcat cattaagccg
    16861 taatgacgac tcataaactc aagattatca atgcgcaggc agcaaaactc ttgcccgcct
    16921 cttgctccgg cgcttgttcc aacgcacgga agttgggcag gagcgttaac ggatcggtca
    16981 gcgcctgcaa atgccagcgg cgattaagcc attcactgcg atgataaatg cgcaccatat
    17041 agagcaggca aacgctaaag gaaatcagca ccgcaagaat aaatgccagc gaatattcgg
    17101 tttccacccc ttgcaaaaag ttctggttgt aattcagaag gcaaagcgtt gaaacagccc
    17161 aggtgagatt taaaaacgga tagcgaagct taccgacccc gagggtaaag atgatgaaga
    17221 aaacgggtac caggtaaccg gcaataaagt cgttttcata aggtgtgcac aacagaagta
    17281 gaagcacgct taacgctgcc agccagctta aggtaaatgc gcgtttctct ttgcccaacg
    17341 acggcgcgat atccctgcgc cacaatatct gcgcaaagtg gggacttaca atcatgcgag
    17401 tgagatagta gaaaagcatg ttgtaaataa gcacagcggt gaaaaggctt agcaaatcaa
    17461 cgaccgtgaa aatggcatcc gcatcgccga aaaaggtgga tatctttagc gggaaatcaa
    17521 agaagctccc cacaagatac atgctgcatt tgatgccgat cggcgtcacc aaaccaagcc
    17581 agaacagacg ttgccagaca tatcggctgg tcaatccata acgccaacgc gtccccagct
    17641 gccagcgtaa tattgcgcag gcacacaaca cgacaaacgt ctggctaccg agcatgaccg
    17701 cagtctgtaa aaaagagaga tgaaagttcc acagattggt gcaaaacatc ccgcataaaa
    17761 cgggaacgac accgcgccag ccaaagataa acaagattga cagcattacg cacagtggca
    17821 tccacgccag aaagatataa ctggaatgga taatggcaag tggcgaaata aaacgggata
    17881 gctgaataag taccacggtg agcgtaaacg ctagtgtgaa tatcttgata ttttttatca
    17941 ggttatgctc cacaaacatg agtacactac tctcactgat cggggttttc aggcacacaa
    18001 ataataagtt tattcgcatc gcgaagcaaa cagggggcca gcctgttgcc gatttttcat
    18061 ccaatgatgc tttcaccagg cggcactaac ccggacgtag actgtattac aaaagcggca
    18121 aaaagcagag acaaaaaacc cccgctttgc agcgagggtt ggaaatttgg tggagctaag
    18181 cgggatcgaa ccgctgacct cttgcatgcc atgcaagcgc tctcccagct gagctatagc
    18241 cccacgatgc gtttacgtac caaatttgct gggtgcaaaa tttggtggag ctaagcggga
    18301 tcgaaccgct gacctcttgc atgccatgca agcgctctcc cagctgagct atagccccgt
    18361 cacgtaaagc ttgtcgagtt gacgggcggc atcatatgaa ttccgcccga atgtgtcaac
    18421 ggcaaattgc aacgtgtagt ttcaatcgct gaaaaatcag gcaaatgaac aattttggaa
    18481 aaccgctcgc attcagtagt tattcatgtc acggtttcct gtaaagtggt gttataaaat
    18541 gaactactaa tagacccaca tacattcagg gaattgttat gttcaaggag aggatgacac
    18601 cagatgaact tgccagactg accggctata gccgccagac cattaataaa tgggtacgca
    18661 aggagggctg gacaacgtca ccaaaacctg gcgtccaggg tggcaaagcc cgactggttc
    18721 acgtcaatga acaggttcgt gaatatattc gcaatgctga acgtccagaa ggtcagggag
    18781 aagcgcctgc cctttctggt gatgcgccgc ttgaagtatt gctggtgaca ctggcgaaag
    18841 agatgacgcc agttgaacaa aaacagttta catccttgct tctgcgggaa gggattatcg
    18901 gattgttaca acgcttaggg attcgcgata gcaaataata tgaaaagatt acgcaataaa
    18961 atgaccaccg aagaactggc tgaatgcctc ggtgtggcta aacaaactgt taaccgttgg
    19021 atcagagaaa aaggctggaa aacggaaaaa tttcccggcg tgaaaggcgg tcgtgccaga
    19081 cttattctgg tcgatacgca agtttgcgag tttattcaga acacgccagc cttccataac
    19141 acaccaatgt tgatggaagc tgaagagaga attgctgaat acgctcctgg agcacgcgcc
    19201 ccggcgtatc gacaaatcat caacgcgata gataatatga cagacattga gcagggaaaa
    19261 gtcgcgcaat ttttgtcacg tgaaggaatt cgcaatttcc tcgcccgtct cgatattgac
    19321 gaatcagcat aaataaaaaa cggcaggata ttatctcctg ccgtttatct ttttacacgc
    19381 taattactgc tgattttcgc gttcagcaat aaaatccagc gctttgttga tacgctcgat
    19441 actgcgggtc ttaccaattg cgtgaacggt aacatccagt gctggagact gccccgcccc
    19501 ggttacggcg acacgcagcg gcataccaac tttacccata cccacttcca gctcatccgc
    19561 cgtcgcctga atagcgtgat gaacgttttc agcggtccag tcagtaatcg cggccagttt
    19621 gtcacgaacc acttccagcg gctgacgcgc taccggacgc agatgttttt tcgccgcatc
    19681 ggcatcgaac tcagcaaaat cttcgtagaa ataacggcag ctctgtgcca tctctttcag
    19741 cgtcttgcag cgttcgccca gcagtttcac cagatcagcc agctgcgggc cgttacgggt
    19801 atcgatattt tcctgctcaa tgtgccactg taagtgagta gcaacatact ccggcggcag
    19861 cgcgttaatg tagtgatggt tcagccacag cagcttgtcg gtgttgaacg cactggcaga
    19921 tttgctgacg gcattcaaag tgaagtattt gatcatctct tcacgagtga agatttcctg
    19981 atcgccgtgg gaccagccca gacgcaccag atagttcagc agtgcttctg gcaaataacc
    20041 gtcatcacga tactgcatta cgctgactgc cccgtgacgt ttggacagtt ttttaccgtc
    20101 atcgccattg atcatagaaa cgtgcgcgta aaccggcacc ggcgctttca gggccttaag
    20161 aatgttgatc tggcgcggcg tgttgttgat atggtcttcg ccacggataa cgtgggtgat
    20221 ttccatatcc cagtcatcga caaccacaca gaagttatag gttggggaac catcggtacg
    20281 gcggataata agatcgtcca gttcctggtt gctgaactcg atcggaccac ggatctgatc
    20341 gtcaaaaaca acagaacctt cctgcgggtt agcaaaacgt acaacacacg gttcatcatc
    20401 agcatgatgc tcatggctgt ggcggcagcg accgtcataa cgcggcttct cacctttcgc
    20461 catttgctct tcgcgcagcg cttccaggcg ctctttagag caatagcatt tataagcagt
    20521 gccctcttcc agcatctgat cgatcaccgc gttgtagcga tcaaaacgtt tggtctggta
    20581 gtacggacct tcatcccact ccaggctcag ccagttcatg ccatccataa tggcttcgat
    20641 agcttccggc gtggaacgct caagatcggt gtcttcaata cgcagcacga actcaccgcc
    20701 gtggttacgt gcaaaaagcc aggagtaaag agcagtacgc gcgccgccaa cgtgcagata
    20761 gcctgttggg cttggcgcga agcgagtttt gattttcatg aaatggcctt acgtttagaa
    20821 agatgccgac aaccggcaaa tcctggaaaa attgagtggg cgatattcta tcactcccgc
    20881 ctgattcctc aatgtagttc gggtttctac cttacgcatt attgttttcg ttgacaaatt
    20941 gcgcaacacg gtctgttttg cgatcatctt gcttaaattt acgacgaacg aacaatttct
    21001 ttagaaaatg cgttgactca ttttgaactc tccctataat gcggctccac acagcggggg
    21061 tgattagctc agctgggaga gcacctccct tacaaggagg gggtcggcgg ttcgatcccg
    21121 tcatcaccca ccaactactt tatgtagtct ccgccgtgta gcaagaaatt gagaagtggg
    21181 tgattagctc agctgggaga gcacctccct tacaaggagg gggtcggcgg ttcgatcccg
    21241 tcatcaccca ccaatttctc gccagctaaa tttcttgtaa aaatgtgaag taccgaagtg
    21301 ggtgattagc tcagctggga gagcacctcc cttacaagga gggggtcggc ggttcgatcc
    21361 cgtcatcacc caccacttcg ggtcgttagc tcagttggta gagcagttga cttttaatca
    21421 attggtcgca ggttcgaatc ctgcacgacc caccaatgta aaaaagcgcc ctaaaggcgc
    21481 ttttttgcta tctgcgatac
//
LOCUS       DAZPLI010000061        21499 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZPLI010000061 DAZPLI010000000
VERSION     DAZPLI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21499
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.061"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            78..2411
                     /gene="bisC"
                     /locus_tag="ACHUU3_002195"
     CDS             78..2411
                     /gene="bisC"
                     /locus_tag="ACHUU3_002195"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFR2082250.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2380..2820)
                     /locus_tag="ACHUU3_002196"
     CDS             complement(2380..2820)
                     /locus_tag="ACHUU3_002196"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR2082251.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2817..3380)
                     /gene="tag"
                     /locus_tag="ACHUU3_002197"
     CDS             complement(2817..3380)
                     /gene="tag"
                     /locus_tag="ACHUU3_002197"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR2082252.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3538..4236
                     /locus_tag="ACHUU3_002198"
     CDS             3538..4236
                     /locus_tag="ACHUU3_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR2082253.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4465..5673
                     /locus_tag="ACHUU3_002199"
     CDS             4465..5673
                     /locus_tag="ACHUU3_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2082254.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5997..7688
                     /gene="eptB"
                     /locus_tag="ACHUU3_002200"
     CDS             5997..7688
                     /gene="eptB"
                     /locus_tag="ACHUU3_002200"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR2082255.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7780..7856
                     /locus_tag="ACHUU3_002201"
     tRNA            7780..7856
                     /locus_tag="ACHUU3_002201"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7814..7816,aa:Pro,seq:cgg)
     gene            7891..8024
                     /locus_tag="ACHUU3_002202"
     ncRNA           7891..8024
                     /ncRNA_class="other"
                     /locus_tag="ACHUU3_002202"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8769..10376
                     /gene="dppA"
                     /locus_tag="ACHUU3_002203"
     CDS             8769..10376
                     /gene="dppA"
                     /locus_tag="ACHUU3_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR2082256.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10684..11703
                     /gene="dppB"
                     /locus_tag="ACHUU3_002204"
     CDS             10684..11703
                     /gene="dppB"
                     /locus_tag="ACHUU3_002204"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR2082257.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11713..12615
                     /gene="dppC"
                     /locus_tag="ACHUU3_002205"
     CDS             11713..12615
                     /gene="dppC"
                     /locus_tag="ACHUU3_002205"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR2082258.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12626..13609
                     /gene="dppD"
                     /locus_tag="ACHUU3_002206"
     CDS             12626..13609
                     /gene="dppD"
                     /locus_tag="ACHUU3_002206"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR2082259.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13606..14610
                     /gene="dppF"
                     /locus_tag="ACHUU3_002207"
     CDS             13606..14610
                     /gene="dppF"
                     /locus_tag="ACHUU3_002207"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR2082260.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14640..15911)
                     /locus_tag="ACHUU3_002208"
     CDS             complement(14640..15911)
                     /locus_tag="ACHUU3_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR2082261.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            16387..16494
                     /locus_tag="ACHUU3_002209"
     CDS             16387..16494
                     /locus_tag="ACHUU3_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR2082262.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16870..16977
                     /locus_tag="ACHUU3_002210"
     CDS             16870..16977
                     /locus_tag="ACHUU3_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR2082263.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            17353..17460
                     /locus_tag="ACHUU3_002211"
     CDS             17353..17460
                     /locus_tag="ACHUU3_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR2082264.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            17836..17943
                     /locus_tag="ACHUU3_002212"
     CDS             17836..17943
                     /locus_tag="ACHUU3_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR2082265.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(18030..19709)
                     /gene="bcsG"
                     /locus_tag="ACHUU3_002213"
     CDS             complement(18030..19709)
                     /gene="bcsG"
                     /locus_tag="ACHUU3_002213"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR2082266.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(19706..19897)
                     /gene="bcsF"
                     /locus_tag="ACHUU3_002214"
     CDS             complement(19706..19897)
                     /gene="bcsF"
                     /locus_tag="ACHUU3_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR2082267.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(19894..21465)
                     /gene="bcsE"
                     /locus_tag="ACHUU3_002215"
     CDS             complement(19894..21465)
                     /gene="bcsE"
                     /locus_tag="ACHUU3_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR2082268.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT         5197 a         5353 c         5780 g         5169 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttgcca atggtggtaa ccccacgcgg cgttctgcgg
     1201 tgctctcttc catgcagggc agcttgccgg gtggctgcga tgcggtggat aaaatccctg
     1261 ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc atatcaacac aacggtatga
     1321 accgacattt cccggatatt cgttttatct ggtgggcggg cggtgccaac tttactcatc
     1381 atcaggatac caatcgtctg atccgtgcct ggcaaaaacc ggagctggtg gtgatctctg
     1441 aatgcttctg gacggcggcg gcgaaacacg cggatatcgt tctgcctgcg actacctctt
     1501 ttgagcgtaa tgatctcacc atgaccggtg attacagtaa tcagcatctg gtgccgatga
     1561 agcaagtggt gccgccacgc tatgaagcgc gtaatgattt tgatgttttt gccgagttga
     1621 gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga aggaaaaagt cagctgcaat
     1681 ggctggaaac gttttataac gttgcccggc agcgcggggc aagccagcag gttgaattgc
     1741 cgccatttgc tgagttctgg caagccaacc agttaattga gatgccggaa aacccggaca
     1801 gcgagcggtt tattcgcttc gctgattttt gccgcgatcc gctggcgcat ccgttaaaaa
     1861 ccgccagcgg caagattgaa atcttctcac agcgtattgc cgattacggt tacccggatt
     1921 gccctgggca tccaatgtgg ctggagccgg acgaatggca gggcaatgcc gaaccagaac
     1981 agttgcaggt actttctgcc catccggcgc accgcctgca cagccagctg aattacagtt
     2041 ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt caccattcat cctgacgatg
     2101 ctcaggcgcg cggcataacc gaaggggata tggtgcgggt gtggaactcc cgtgggcaga
     2161 tccttgccgg agcggtcatt agcgagggaa ttaaacctgg cgtgatttgc attcacgaag
     2221 gggcatggcc ggatctggat ttaaccgctg acggtatttg taaaaacggc gcagtgaacg
     2281 tgctgaccaa agatctcccc agctcgcggc tggggaatgg ctgtgcgggt aatacggcgc
     2341 tggcatggct ggaaaaatac aacggtccgg aactgacact tacagcgttt gaaccaccgg
     2401 ccagctcata atccaggtgg gtagttgggt ttcatcctgc catgcgcaat cgacaatgtg
     2461 aaaaccctgc gcacggtaaa aatctatcgc cggttgattt ttttgataaa cctccagcat
     2521 cagatgggga tagcgctgct gcacatactg catcagcgcc ttaccaatac cgcgtctgac
     2581 ggccttcggt gcgacaaaca ttgctgccag aaatcggcct tccataatgc tgacaaaacc
     2641 gagaagctta ccgtcctctt cccagaccca gttttgcgcg ttggcaagat aggcatcccg
     2701 caccagcgga atgcagtcac gccagtaatt cgcttttata aagggatgcc cccaggttgt
     2761 actttccagc cacagttcga ggatcgcggg gagttctgaa cgttgcgctt cccgaatcat
     2821 ggtttatttc ccggatagca acagcagcca accacatgat cattcaccag cccacatgcc
     2881 tgcataaagg agtaacagat tgtggtgccg acaaacttaa aaccacgttt tttcagtgct
     2941 ttagataggg cgtcggaggc ggacgtagat gtgggaattt cgctcaacgt tgtggcttgt
     3001 gtcacctgtg gctgatgatt tacaaacgac cagacaaagt cggcaaacgg ttcgccgttc
     3061 tgttccattt gcaggtacgc ccgcgcatta ccaataattg cctgaatttt ccctcgatgg
     3121 cggataatcc cggcgtcctg taccagtctt tcgacatcct cttcctgcat tgctgcgacc
     3181 ttcaccggat cgaactgatg aaagcaggcg cgatagtttt cgcgtttttt gaggacggtg
     3241 atccacgata acccagcctg ctgcccttca aggcagatca tttcgaacag ttttttactg
     3301 tcagtttcag gcacgcccca ttcattatca tggtaggcaa tataaagcgg gtcctgactc
     3361 acccagccgc aacgttccat actttccctc gctatgtacc cgatcgtaaa aatttcagcc
     3421 gcttttcttg acgcatcggt gaaaaagtca atatttaata cagggctact atgcccgata
     3481 tccttcatac aatgacaata atattgccga attcggctct tccctggagt cgtgttaatg
     3541 atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc tggcgagcgt ggtaatcagt
     3601 gccttttttc tcaacacagc ttacgcctgg caacaagaat atatcgttga tacgcaaccc
     3661 ggacattcca cagagcgtta cacctgggat agtgatcatc aacctaatta caacgatatt
     3721 ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac tggaagtcaa tctggcggaa
     3781 gaaactcctg tggatgtgac cagcagtatg agtatgggct ggaattttcc tttatatgaa
     3841 caggttacaa ccggcccggt cgcggcatta cattacgatg gcacaaccac ctcgatgtat
     3901 aacgagtttg gcgacagtac taccacgcag accgatccgt tatggcatgc cagcgtgagt
     3961 accttaggct ggcgtgttga ctcccggctt ggcgatctcc gaccctgggc gcaaatcagc
     4021 tataaccagc aatttggcga gaatatctgg aaggcgcaat caggcctgag ccggatgacg
     4081 gcgacaaacc agaacggcaa ctggctggat gtcaccgtag gcgctgatat gttgctcaat
     4141 caaaatattg ccgcctatgc cgcgctaact caggcagaaa ataccactaa taatagcgac
     4201 tatctgtata cgatgggggt tagcgccaga ttttaacgta acagtcacaa ttgaaaccat
     4261 taaataacaa taattgtggc gatagtgggt gctaacttac caaataataa atttggtgaa
     4321 taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga tggcatttca
     4381 tgccgttttt ccccaggcat aaagtgcact tcgttatggt tgtcggcaga gatttttcct
     4441 ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac ccgctggctg
     4501 acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac ctggagcctg
     4561 tttaatggcg cgctttccgc caagctggat gcgccggtaa gccaggtcgc tttctctttc
     4621 ggcttgttaa gtctggggct ggcaatttcg tcttctgttg cgggcaaatt acaggaacgt
     4681 tttggcgtta aacgcgtcac catggcttcc ggcattttgc tgggattagg cttcttcctg
     4741 accgcgcatt ccaacaacct gatgatgctg tggttaagcg ccggtgtgct ggtcggtctg
     4801 gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt cccggagcgt
     4861 aaaggtctga tctccgcgtt cgctatcggt tcttatggtc tgggtagcct gggtttcaaa
     4921 tttatcgaca cgcagctgct cgaaacggtg ggtctggaaa aaacatttgt gatttgggga
     4981 gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc accaaaacag
     5041 gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga gtcgatgcgt
     5101 aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag cggcctgtac
     5161 gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt ggtttccgca
     5221 gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt gctgggcatt
     5281 ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat ttcgctggtg
     5341 ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc agcgattgcc
     5401 tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt cagtgagttc
     5461 ttcggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt cggtatcggt
     5521 agcatttttg gttcgattat cgcctcactg tttggcggtt tctatgtgac cttctacgtg
     5581 attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca gccagagcag
     5641 aaaatgttgc gtgaggcgca tggctccctt taatgctcag ccgcgagcta ctgggcttca
     5701 gcttccgtgt gcatcaaacg catcaacgaa atgtataaac aatgactcgt aaaatttggg
     5761 ccagttaact ggcccttttt actgcttttt gtaacgctaa agtctctttt caaacttgca
     5821 tttttgtaaa tttgtgcttc atgcacactc tttccccaca ctttttcgct ttgctgtggt
     5881 ctacttattc gcgcgtgtag attttactta tctgactacc tccgcacttt tcccctgccg
     5941 ggcctgaaaa gccgttaagc agggtgttat cacctgtttg tccagggttt gtttgcatga
     6001 gatacatcaa atcgattaca cagcagaagc tgagcttttt gcttgcaatc tatattggcc
     6061 tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac gattttaccg
     6121 tctggaaagg cgtttctgct gttgttgaac tggccgccac cgtactggtg accttctttt
     6181 tactacgtct tctatcgctg tttggccgcc gcagctggcg tattctggcg tcgctggtgg
     6241 tgctcttttc cgcaggtgcc agctattaca tgaccttcct taatgtggtc attggttatg
     6301 gcatcatcgc ttccgtcatg accaccgata tcgacctgtc aaaagaagtt gttggtctga
     6361 actttattct ctggttaatc gccgttagtg cattgcctct tatccttatc tggaataacc
     6421 gctgtcgcta caccttgctc cgacaactgc gaaccccagg gcagcgtatt cgcagcctgg
     6481 cggtcgtcgt actggcgggt attatggttt gggcaccgat tcgtttgctg gatatccagc
     6541 agaagaaagt ggagagggcg accggcgttg atttgccgag ttatggcggt gtcgtagcga
     6601 actcttatct gccatcaaac tggctttctg cgttggggct gtatgcctgg gcgcgggtcg
     6661 atgaatcttc cgataataat tcattgctta atccggcgaa gaagtttaca taccaggctc
     6721 cacaaaacgt tgatgatact tatgtcgtgt ttatcatcgg tgaaaccacg cgttgggacc
     6781 atatgggtat tttcggctat gagcgtaata ccacgccgaa actggcccag gagaaaaatc
     6841 tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa actctcactg cgttgcatgt
     6901 ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac attaaaagaa cagaacattt
     6961 tcgcggttct gaagcagtta ggattcagtt ctgacctcta cgctatgcag agcgaaatgt
     7021 ggttctacag caacacgatg gcggacaaca ttgcttatcg tgagcagatt ggtgcggagc
     7081 cacgtaatcg tggcaagccg gtagatgata tgttgctggt agacgaaatg cagcaatcgc
     7141 tagggcgcaa cccggatggt aagcatctga tcattctgca taccaaaggt tcgcatttta
     7201 actacaccca gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt attggtgttg
     7261 atagcggctg taccaaagcg cagatgatca actcctatga caactcggtg acctatgtgg
     7321 atcactttat ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt gtgttctacg
     7381 cagctgacca cggtgagtca attaatgaac gcgagcacct gcacggcacg ccgcgtgaac
     7441 tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg gatgtcagat aaatatctgg
     7501 aaaatccggc caatgcgcag gcgtttgcgc agctgaaaaa agaagccgac atgaaagtgc
     7561 cacgccgtca cgtagagctg tacgacacca tcatgggttg tcttggctat acttcaccgg
     7621 atggtggaat taacgaaaac aacaactggt gtcacatccc gcagacaaaa gaggcagcgg
     7681 ctaactaaga ggcctgctga ctttctcgcc gatcaaaagg cattttgcta ttaagggatt
     7741 gacgagggcg tatctgcgca gtaagatgcg ccccgcattc ggtgattggc gcagcttggt
     7801 agcgcacttc gttcgggacg aaggggtcgg aggttcgaat cctctatcac cgaccaaatt
     7861 cgaaaagcct gctcagcgag caggcttttt tgcatttgaa gcccatgagg atgagaacct
     7921 ccgggggcgg ggaggttcga ctcgagcgaa gcgagagaac gttgcgccag caacggcccg
     7981 cagggcgagc cacgaagtgg cgagtaatcc tctatcaccg accaaattcg aaaagcctgc
     8041 tcaacgagca ggcttttttg catttgtagc tcatgaggat gagaacctcc gggggcaggg
     8101 aggttcgact cgacgtaggc ctgataagac gcgccagcgt cgcatcaggc aacggcaccg
     8161 catttattgt cggatgcggc gcgaccgcct tattcgacca acacaccaca cattttcctg
     8221 ccaactttgc gccccatatc tcactatcca ttaacggatt tgtgacagaa accattgtct
     8281 ttttttatat ttgcttaaat ctttttttgc gttacttatg gctggcgctg gcattttccg
     8341 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
     8401 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
     8461 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
     8521 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
     8581 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
     8641 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
     8701 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
     8761 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
     8821 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
     8881 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
     8941 taccgcttta taaccgcctg gttgaattta aaatcggcac cactgaagtg atcccgggcc
     9001 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
     9061 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg tgaactgaac gccgatgacg
     9121 tggtgttctc gttcgaccgt cagaaaaacg cgcaaaaccc gtaccataaa gtctctggcg
     9181 gcagctacga atacttcgaa ggcatgggct tgccggagct gatcagtgaa gtgaaaaagg
     9241 tggacgacaa cacagttcag ttcgtactga ctcgcccgga agcgccgttc ctcgctgatc
     9301 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgatgcgatg atgaaagccg
     9361 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
     9421 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
     9481 agatcgatac gctggttttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
     9541 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
     9601 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcctata
     9661 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca ggcactgacc tacgcggtga
     9721 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
     9781 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
     9841 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
     9901 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
     9961 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    10021 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    10081 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    10141 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    10201 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcaga
    10261 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    10321 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    10381 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    10441 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    10501 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    10561 cctctgcgtg cagcgccatc cggcagcaat acttcattcc ctgtccgata agggcgggaa
    10621 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg ggtcactata gagaatccgg
    10681 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    10741 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    10801 ggcgaacgcg ggatctcccc tgagcgtcac gcgcagctgc tggctgaact cggcttagat
    10861 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    10921 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    10981 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    11041 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    11101 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    11161 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    11221 ccgttaaccg gttttatgct gatcgacacc gccatctggg gtgaagatgg caactttatc
    11281 gacgccgttg cccatatgat cttaccggct attgtgctgg gtactatccc gctggcggtt
    11341 atcgttcgta tgacgcgctc ttcgatgctg gaagtgctgg gtgaagatta catccgcacc
    11401 gcgcgcgcca aagggttgac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    11461 atgctgccgg tagtgaccgt tatcggcctg caggtgggaa cattgctggc gggggcgatt
    11521 ctgaccgaaa ccatcttctc gtggcccggt ctgggacgct ggttgattga cgcactgcaa
    11581 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    11641 gtcaacctgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    11701 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    11761 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt tgtcgggctg
    11821 gtttacatcg tcatcgtgct gtttatcacg atctttgcca actggattgc gccttataac
    11881 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagc
    11941 atggcgcact tgctgggcac cgatgacgta ggccgtgatg tgctgtcgcg gctgatgtac
    12001 ggcgcgcgct tgtcgctgct ggttggctgt ctggtggtgg tgttatcgct gattatgggc
    12061 gttattctcg gcctgatcgc cggttacttc ggcggcctgg tcgataacat catcatgcgc
    12121 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    12181 ttcggcccgt cgattggtaa cgccgcgctg gcactgacct tcgttgcctt gccgcactat
    12241 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt taccgcgtct
    12301 cgcgtggcag gtgccggggc gatgcgtcag atgtttatta acatcttccc gaactgcctg
    12361 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggccgcc
    12421 cttggcttcc tcggcatggg agcgcagccg ccaacaccag agtggggcac catgctctcc
    12481 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttcccggg tctggcgatc
    12541 ctgctgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    12601 aaactgaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    12661 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    12721 gggcgaagtg gtcgggattg tgggtgagtc tggctccggt aagtcggtca gttcactggc
    12781 gattatgggg ctgattgatt atccgggccg cgtaatggcg gaaaagctgg tgtttaacgg
    12841 tcaggatttg cagcgtatat cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    12901 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    12961 catggaagcg attaaggtgc atcagggcgg taataagagc actcgccgtc agcgggcgat
    13021 tgacctgctg aatcaggtcg gtattcccga tccggcatcg cgtctggatg tttacccgca
    13081 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    13141 aaaactgctg attgccgatg agccgaccac tgcgctggac gtgaccattc aggcgcaaat
    13201 catcgaactg ttgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    13261 tgatctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggccaggt
    13321 ggtggaaacg ggcgatgcgc acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    13381 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    13441 tgtcgttccc ggcaagtacg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    13501 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg acgggcgcca
    13561 gtccaaatgc cattacccac ttgatgatgc cgggaggccg acactatgag tacgcaagag
    13621 gccaccctgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    13681 aaaggcatgt tcgcgccgga acgtctggtt aaagcgctgg atggcgtttc gtttaacctt
    13741 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    13801 cggttgctga cgatgattga aacgcccacc ggtggcgagc tgtattacca ggggcaggat
    13861 ctgcttaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    13921 cagaaccctt acggttcgct gaatccgcgt aaaaaagtcg ggcaaattct tgaagagccg
    13981 ctgctgatca acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    14041 gcgaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcatatgtt ctccggcggt
    14101 cagcgtcagc gtatcgccat cgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    14161 gatgaaccgg tttccgcgct ggatgtttca gtgcgcgcgc aggtgctgaa tctgatgatg
    14221 gatttgcagc aggagttggg gctgtcttat gtctttatct cccacgacct gtcggtggtg
    14281 gagcacattg ctgatgaagt gatggtgatg tacctgggcc gctgcgtgga gaagggaacg
    14341 aaagaccaaa tcttcaataa cccgcgccat ccgtacactc aggcgctact ttccgcgacg
    14401 ccgcgcctga acccggacga tcgccgcgag cgcatcaagc tcagcggtga actaccaagc
    14461 ccactgaatc caccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    14521 tgcacccagt tgcagccgca gctaaaagac tacggcggtc aactggtagc ttgttttgct
    14581 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    14641 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtacag cagaatcagc
    14701 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    14761 atgaggcacg acacaatacc atataacggg cttcccaact gaaagaacac cagcaccgat
    14821 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    14881 agcacgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    14941 cctttaatgg cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    15001 atattaagca ccgtagaggt gatatgaatg atatgcccag ggatcacctg cgcggccagc
    15061 gccagtgctg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    15121 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    15181 gtacgcagcg cgagccgggt tgccagtact ttatccgctt cccgtttacg ataggcaata
    15241 ttcattgggt taagtacctg aataaatact gcagaaaaga agcaaaatgg aatggtaagc
    15301 aagacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    15361 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    15421 ccggaaatct taaacagtaa tcgttcacca ccagacgcaa tcgccaccag tacggcgaaa
    15481 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    15541 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    15601 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    15661 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    15721 actacccagg tggcaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    15781 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    15841 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg
    15901 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    15961 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    16021 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    16081 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    16141 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agcaatcgta aaatcagcgg
    16201 caagccgaaa gggttccggt gaggcgcaat gttgcggggg ctttatccct ggtggcattg
    16261 gttgctggag agagaaaacc cccgcacgtt gcaggtatgt acctgacaac ataacggggg
    16321 ctaatcttga acctagacca ctcaagaata gccgcgaaac gttgtcatta caacacaggc
    16381 ggctatatga cgctcgcaga gctgggcatg gccttctggc atgatttagc ggctccggtc
    16441 attgctggca ttcttgccag tatgatcgtg aactggctga acaagcggaa gtagcgtgtc
    16501 atgtgggcgt caggctgccg taatggcaat ttgcgcccgg accaggccgc aggagtgaaa
    16561 ctctgcggcc tttttcgtaa gaaaaatgca acggttgcaa gcggataata ttctgcggaa
    16621 tgcgcagcac agagtgaaga tgagacggat tccggaacag aaaaacagtc gtagaatcag
    16681 cgtcaagccg gaaggattcc ggtgaggcgc aatgttgcgg gggcttaatc cctggtggca
    16741 ttggttgctg gagagagaaa acccccgcac gttgcaggta tgcacctgac aacaccacgg
    16801 gggccattct tgcatccaaa caatacaaga atagccgcga aacgttgtca ttacaataca
    16861 ggcggctata tgacgctcgc agaactgggc atggccttct ggcatgattt agcggctccg
    16921 gtcattgctg gtattcttgc cagtatgatc gtgaactggc tgaacaagcg gaagtaacgt
    16981 gccatgcggg cgtcaggctg ccgtaatggc aatttgcgcc cggatcaggc cgcaggggtg
    17041 aaactctgcg gcctttttcg taagaaaaat gcaacggttg caagcggata atattctgcg
    17101 gaatgcgcag cacagagtga agacgtgacg gattccggaa cagaaaaaca gtcgtagaat
    17161 cagcgtcaag ccggaagggt tccggtgagg cgcaatgttg cgggggcttt atccctggtg
    17221 gcattggttg ctggagagag aaaacccccg cacgttgcag gtatgcacct gacaacatca
    17281 cgggggccat tcttgaacct gaacaactca agaatagccg cgaaacgttg tcattacaac
    17341 acaggcggct atatgacgct cgcagaactg ggcatggcct tctggcatga tttagcggct
    17401 ccggtcattg ctggcattct tgccagtatg atcgtgaact ggctgaacaa gcggaagtaa
    17461 cgtgtcatgc gggcgtcagg ctgccgtaat ggcaatttgc gcccggacca ggccgcaggg
    17521 gtgaaactct gcggcctttt tcgtaagaaa aatgcaacga ttgcacgcgg ataatattct
    17581 gcggaatgcg cagcacagag tgaagacgtg acggattccg gaacagaaaa acagtcgtag
    17641 aatcagcggc aagccggaaa ggttccggtg aggcgcaatg ttgcgggggc tttatccctg
    17701 gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg caggtatgca cctgacaaca
    17761 tcacgggggc taatcttgaa cccaaacaac tcaagaatag ccgcgaaacg ttggcattac
    17821 aacacaggcg gctatatgac gctcgcagaa ctgggcatgg ccttctggca tgatttagcg
    17881 gctccggtca ttgctggcat tcttgccagt atgatcgtga actggctgaa taagcggaag
    17941 taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt tgcgcccgga tcaggccgca
    18001 gaggttaaac tctgcggcct ttttcgtgct tactgcgggt aaggcaccca gtcgccgccg
    18061 ttcaggcgaa cgtacggttt atcctggtat tgaataacta ctgcatttga gttctcggag
    18121 accggtgctg tttgtggcaa cccactggtg agttttttcc agtcaacatt gtcttcggtg
    18181 aaaatcttgc catcaagaac gcgaaccacc agatcggaga tagctaggaa gctgctcggt
    18241 tggtcaatga caatcggtgc cccctgatgt ggtgccttca tgccgaagaa tttcacccca
    18301 acggggacgt ctgtgataga cgggctaggg atatcacgta ggccagatac ctgcattctg
    18361 tcgcccttca gcgcgccgcc gtgttccggc accacgacca ccatcacttt acgacccgat
    18421 ttctccagtt cagtaaagaa ggcgtccagt tcatcaaaga atttctgcgc ccgcgctttg
    18481 taatccgctg ttttgctgac ccccggataa tggttgccgt catgcagtgg aagcgtgttg
    18541 tagaacgtgg cactacggct gtttttatct ttttcggtaa cgtccagcca gcggttaagc
    18601 acggcggtat cgtcataaac cggcgaacca tcaaagccca gcaaaataac cggcagattt
    18661 gtttgatcca tcaattcagt ctgcatgccg ccattttcgc gaacttcttt caaaaaaccg
    18721 ccgaactggc cgttatgtcc catcatcagg tgctgggtaa agcccagttt cgaaaggtta
    18781 tcaaacagat agcagtcgtt atttgccggt tgatacagat tagtgtgcga agtctgcccg
    18841 cagctggcgc gcagtaaacg gatcgccgcc gggccactgt aggaggtggc ggagttaaag
    18901 ttcttgaact caatatcgaa atgcgaccac agtggatgcg acatcaaccc ggcggcttct
    18961 atatccgacc aggaaagcga acagatgtta atcaccagta gttcaaatgg ctgagcatca
    19021 gcgggcagcg aagacgggaa ggtcgattta cgtttcgcct ccgcgttata gaaattattc
    19081 agccaggcgt taaggttcgc cgttgttggc ggtgcagttt gtgcgggcat atcacccact
    19141 accgatgcgc cacccgtcgc cgcaacggtt gccgctgcgt taccacccgt cgttgttaca
    19201 gtggtcgtcg gttgtccggc tggccacaag gagaaacttg gtcccgccag ggtaagtacg
    19261 ttcagccata gcagtatggc aaccacaaaa acggtaatgc gaatccattg tgacaggaat
    19321 aaccaggcca ctaataaaac aaaaatggcc ccaatcatct gccagttaat aaagcgtgtg
    19381 acaaggtcga ttaaataatc ggtactgaac cccgccacct gcgaaccctg gctcattatg
    19441 ctttccgggc caggcaacca ggtgtcatgc cagaacaaag caaagccgat cggcagggca
    19501 atccagtggc gcaagcgatg caggctgtag cgcggaatgg gcatcagcag aaacgtggca
    19561 aacaccaaat tgaggagcgg atggaagtta agatatcccg cccacaacag gccgaactta
    19621 accagaaaat agaagttcca gccggaaagg ccgcgccagt attgccagag ggaagaaggc
    19681 atggcggtat tttgcgtaaa ttgagtcatt ttttggttgc cctggctttt tccgtgcggc
    19741 gtaacgtccc ggccggttta acataacgag gtttagcaaa gaataaacgt aaggtgtcgc
    19801 gaatgcgtcg caaagagtgc cgcgccagat agcccagcgg gaaaaatatc agtgcgcaaa
    19861 cgacaataat ttcaatgata tcgctgatgg tcatcatgat gagcgctcca cagcatcatc
    19921 taacagtcgc atgggttctg gtattcgtcg ccagtggcga ccatcgtgct cggcattgat
    19981 gaccggttta gaactttgcg ttaaaggcag cggcatgccc cattgttctg gggcaagcaa
    20041 gcgcatctgc accagctcgg cactgatttg atcatcttca aaccagacca tacggtttga
    20101 gaaaatgtcg ccagtaggca atgggaaaat atgattcaac gcggtatcca gatcgttaat
    20161 ccgacagaat gagagaaaca gcaccagccg attaccgcca atggtcatga tatcgccggt
    20221 acggttaggg cgacacagcg tcagggcttg ttcaacgcgg atacccggta ccggacgtag
    20281 ggcaaccaga acgcctttac cgtgggcagg taatagaggg ttattcatca tgttgttgac
    20341 ggcattacag aacacatccc acttctggaa accacgcagt ttgagcggct gggtcattga
    20401 cagcaaggta gtgatatctt ccggcacata gcgactaaac ttctgccctt gcacgctttc
    20461 gatcatcgtc agacaacggg agagtggcgc attccacgga ataaccatat ttgcaccgca
    20521 ggccaataac aaacgttcat cggtggcgcg caggctagcg gtattttccc gcacgaggat
    20581 tttcatcgca ctaccgcgct ggcgacgcag ggtatgaatg ctgcgggcca gtggctcgat
    20641 ttgcgcattt tgctgtaaag aaaagaccac cgtcgccgcc tgagcggtac gggcttcatt
    20701 gaacaggact tcgttattgt tgaacagttg ccagtgttcc gatagcggcg gcgcaccttc
    20761 cagtacagca acattactca gaatgcgttt ttcgtcgctg cgtggttgga tctccgcctc
    20821 ttcgctttga actaatgtcc agataccatt ttgttgctga acgctaagct gctgacgggc
    20881 gctgacccct ttttcgttgc accagaaggc aatatccagc aaatgttggt caccctgaaa
    20941 acgcaaactg gcaagaccaa aaagtgaacg gtattcctca agcaacaatg aaaattgttt
    21001 atcgttatta tttccgggat taattaccaa aagcgaacaa tggtttaacc tgctccattt
    21061 attcatttta tccaaccatg agcgaagccg ctcggcagga atgttttgcc atgcgttatt
    21121 tgcgcaaaca agaataaaaa ggtaattatg gggatcaata gaacactgca aatcacgggc
    21181 caaatagtat agaccttttt catgatttag cattgaaaat aattttattt tttccggccc
    21241 ttgagaatca tctaattgaa agattttcgc cggatcgctg tccatgctaa tgaccgcgac
    21301 gtgtgcggtt tcagcctggg atgcaattgt ttgattcgcc agactgatag catcttcatg
    21361 gcgatcgacg ttaaaccacc agacgccgcc tgctggcata tgtcgcagct catcccataa
    21421 tgatgagata ccgatagaga atacagggtc cacaatgtcc ctcatgtcac cattttgtct
    21481 gattatcagt ttactagcg
//
LOCUS       DAZPLI010000062        21478 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZPLI010000062 DAZPLI010000000
VERSION     DAZPLI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21478
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.062"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(354..899)
                     /gene="hemG"
                     /locus_tag="ACHUU3_002216"
     CDS             complement(354..899)
                     /gene="hemG"
                     /locus_tag="ACHUU3_002216"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR2082269.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(911..2362)
                     /gene="trkH"
                     /locus_tag="ACHUU3_002217"
     CDS             complement(911..2362)
                     /gene="trkH"
                     /locus_tag="ACHUU3_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR2082270.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2401..3015)
                     /locus_tag="ACHUU3_002218"
     CDS             complement(2401..3015)
                     /locus_tag="ACHUU3_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR2082271.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(3015..4346)
                     /gene="pepQ"
                     /locus_tag="ACHUU3_002219"
     CDS             complement(3015..4346)
                     /gene="pepQ"
                     /locus_tag="ACHUU3_002219"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR2082272.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQTPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4536..6725
                     /gene="fadB"
                     /locus_tag="ACHUU3_002220"
     CDS             4536..6725
                     /gene="fadB"
                     /locus_tag="ACHUU3_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR2082273.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLSMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6735..7898
                     /gene="fadA"
                     /locus_tag="ACHUU3_002221"
     CDS             6735..7898
                     /gene="fadA"
                     /locus_tag="ACHUU3_002221"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR2082274.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(8088..8789)
                     /gene="fre"
                     /locus_tag="ACHUU3_002222"
     CDS             complement(8088..8789)
                     /gene="fre"
                     /locus_tag="ACHUU3_002222"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR2082275.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8836..10329)
                     /gene="ubiD"
                     /locus_tag="ACHUU3_002223"
     CDS             complement(8836..10329)
                     /gene="ubiD"
                     /locus_tag="ACHUU3_002223"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR2082276.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10496..10984
                     /gene="rfaH"
                     /locus_tag="ACHUU3_002224"
     CDS             10496..10984
                     /gene="rfaH"
                     /locus_tag="ACHUU3_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR2082277.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10981..11763)
                     /gene="tatD"
                     /locus_tag="ACHUU3_002225"
     CDS             complement(10981..11763)
                     /gene="tatD"
                     /locus_tag="ACHUU3_002225"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR2082278.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(11805..12581)
                     /gene="tatC"
                     /locus_tag="ACHUU3_002226"
     CDS             complement(11805..12581)
                     /gene="tatC"
                     /locus_tag="ACHUU3_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR2082279.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12584..13099)
                     /gene="tatB"
                     /locus_tag="ACHUU3_002227"
     CDS             complement(12584..13099)
                     /gene="tatB"
                     /locus_tag="ACHUU3_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR2082280.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(13103..13372)
                     /gene="tatA"
                     /locus_tag="ACHUU3_002228"
     CDS             complement(13103..13372)
                     /gene="tatA"
                     /locus_tag="ACHUU3_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR2082281.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13451..15091)
                     /gene="ubiB"
                     /locus_tag="ACHUU3_002229"
     CDS             complement(13451..15091)
                     /gene="ubiB"
                     /locus_tag="ACHUU3_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR2082282.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(15088..15693)
                     /gene="ubiJ"
                     /locus_tag="ACHUU3_002230"
     CDS             complement(15088..15693)
                     /gene="ubiJ"
                     /locus_tag="ACHUU3_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR2082283.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15707..16462)
                     /gene="ubiE"
                     /locus_tag="ACHUU3_002231"
     CDS             complement(15707..16462)
                     /gene="ubiE"
                     /locus_tag="ACHUU3_002231"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR2082284.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16557..17984)
                     /gene="rmuC"
                     /locus_tag="ACHUU3_002232"
     CDS             complement(16557..17984)
                     /gene="rmuC"
                     /locus_tag="ACHUU3_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR2082285.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(18125..18886)
                     /gene="udp"
                     /locus_tag="ACHUU3_002233"
     CDS             complement(18125..18886)
                     /gene="udp"
                     /locus_tag="ACHUU3_002233"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR2082286.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            19148..19960
                     /locus_tag="ACHUU3_002234"
     CDS             19148..19960
                     /locus_tag="ACHUU3_002234"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR2082287.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            20354..21037
                     /gene="tssD"
                     /locus_tag="ACHUU3_002235"
                     /pseudo
     CDS             20354..21037
                     /gene="tssD"
                     /locus_tag="ACHUU3_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065915.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
     gene            21017..>21478
                     /locus_tag="ACHUU3_002236"
     CDS             21017..>21478
                     /locus_tag="ACHUU3_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2082288.1"
                     /translation="MAGASIVAGAVLGRMRLPDLESLEHFGARGAVSGRPFNPELAGG
                     PIENLTTDGVTINREGIAIVEKHIARFGHDPVNEVMINRLKDIEKGKIPPEQVDLNFY
                     THECREYQRYCNLGWETGQPDGDAGYALWNHTGFAPIFPDICYHLTHYKPAA"
BASE COUNT         5271 a         5744 c         5379 g         5084 t
ORIGIN      
        1 aacttcgtaa tgaattacgt gtccactctt gagacttggt attcattttt cgtcttgcga
       61 cgttaagaat ccgtatcttc gagtgcccac acagattgtc tgataaattg ttaaagagca
      121 gtgccgcttc actttttctc agcggcgcgg ggtgtgcata atacgctttc ccgctacaga
      181 gtcaagcatt tatttttgct ttctctgtcg gaattctcag gagaaccccg ctgacccggc
      241 ggcgtgtttg ccgttgttcc gtgtcagtgg tggcgcatta tagggagtta ttccggcctg
      301 acaagaggaa atttaaaata attttctgac cgcgcatttt ttattcttta tgcttatttc
      361 agcgtcggtt tgttggttaa ctgggcgatt tctcgggcga aattcgccac ctgctcccaa
      421 tcggtataga caacttcttt gcgcgtatcc gtttcaccgc ctgacatctt cataattagc
      481 ttgatcataa aacgatcgta ccagcgataa cgtgggtaac gcagcgcccc ggcaatgacc
      541 gcgcagcgat cgggacgcca ttgcgagttc atcagaaact tccgcgcgta gctgttggtc
      601 tgtggagtac gcttctccgg tttgcgcgcc accaggttta cggaataaaa ggcgctcggc
      661 atcgaattca gtcgcgtcgc atgttttttg acaaattcct ggaacgcaga gtggtagtga
      721 ccatagcgaa tagaagcacc aatgaccaca cggtcataat tttcccattg tggttcttca
      781 atgcggtgca cattggcgac atccgtctgg atccccagtt ctttcagttc cgaagccagg
      841 taggaggcaa tctcgcgcgt ttgtccgtcc cttgttgaga aaagaattaa tgttttcacg
      901 tattactcca tcattcacgc cagaaagtcg gggtaaagag caccagcaat gtaaagacct
      961 cgagacgacc aaacagcatg ttggcaatca ggatccattt agccaccggg ttcatactgg
     1021 taaagttatc agcaaccacg ccaagccctg gccccaggtt attcaatgtc gcaacaactg
     1081 aagcaaaggc agaaaagtca tccacgcccg tggcgataat cgccagcata ctgacaataa
     1141 acaccaatgc ataggcggag aaaaatcccc aaacggcttc gaggatacgt tccggcagtg
     1201 cgcgattccc cagcttaatg ctatagacgg cgttcggatg caccagtcgt ttcagctcac
     1261 ggttcccctg cttaaacagc agcaggatgc ggatcacttt caggccaccg cccgttgacc
     1321 cggcgcaacc gccgataaaa gctgaacata aaagcagtac cggcaaaaag agcggccagc
     1381 gggcaatgct gtcagtcgta aacccggcgg ttgtcgccat cgataccacc tggaaaaacg
     1441 cctggttaat tgtcatcagc gccgaactgt agacattatg aaaccacagt acgagtgtac
     1501 aaataaccac cagcgtaaac tgtacgccga taaacatgcg aaattccgga tcgcgccaat
     1561 aaaccttcag actacgccca cttaacagtg aaaagtgcag accgtagtta cagccggaga
     1621 tcagcaggaa gatagcaatg atagtgttaa tagtcgggct gtcgaaataa ccgatactgg
     1681 catcatgtgt cgagaagccg ccaatagcga tagtcgcaaa gctatggccg atggcatcaa
     1741 aggcatccat tccggcaaac cacaacgcca gcgcacaggc gacggtcagc aagacataaa
     1801 tcaaccacag ggttttcgcc gtttccgcaa ttcgcgggcg cattttgtta tctttcagcg
     1861 ggccgggcat ttctgcgcga tagagctgca tcccacccac gccgaggata ggcagtatcg
     1921 caaccgctaa cacgatgatc cccatcccgc caaaccattg cagcatctgg cgataaaaga
     1981 ggatggcatg agggagcgaa tccagcccca ccagcgtagt ggccccggtg gtggtcaggc
     2041 cagagaaaga ttcaaaaaac gcatcggtaa tcgtgaggtt cgggctttcc gagaagataa
     2101 aagggagcgc accgacacta cccagcacgg tccagaacag caccactatc agaaacccct
     2161 cacgggattt cagttcgccc ttctctttac ggttcggcca ccacagcata gaaccaatgg
     2221 cgagggcgac aaaaaaggtc tgggtaaaag cgcggcccgc tccatcccgg tagatgagtg
     2281 ctaccagccc agggataatc atggtccctg aaaataagat gaccagtagt ccaacgattc
     2341 gggtaatggc gcgaaaatgc atctctgccg cttccttagt tctgcaaaac gaagtgggga
     2401 ttattcttca atcgctaaca attgcaatga accacggcta aaatccgcca gctttgcgga
     2461 aaattcagcc actttcgccg ccggaagcgc cacccgcagc agaacgaatg cctgataatc
     2521 actgttgata attttgccgt cacactgccc cagcaacgct tcaatgccgg ttaactgatg
     2581 atattcacat tgcaaagtat attcggttaa tggcgtcttg cgttgggtcg ttagctggcg
     2641 cagcgcctga ttcacgccgc cgccatacgc tttcactaac ccaccggtgc caagcaatat
     2701 gccgccgtag tagcgcacta ccacagcggt aatttccccg acgccgctgc ccattagctg
     2761 ggcgagcatc ggtttacctg ccgttcctgc cggctccccg tcgtcagaga aacccagctg
     2821 ttgagaatca tccggcgcac ccgccaccca cgccacgcaa tggtggcggg catcggggtg
     2881 ttctgcccgc accgattcaa caaacgcttt cgccgcctca acgccatcgg tatgcgccaa
     2941 catcgttatg aagcggcttt ttttgatctc ttcaacgacc gtgaccggtg ccgcaggaat
     3001 taaccagctt tccatcacgc cagtttcaga tcccgggtca tgttttccac gttgttttcg
     3061 tggatcacca cgttgtcttc gatacgaatg ccgccgaacg gtttcagtgc ttcaattttc
     3121 tgccagttga agtgcttgct gaactgccct tcacgccacg gtgccagtag cgattcaatg
     3181 aagtagatac ccggttcgat ggttaacacc atacccggct ggagaatacg ggtgcagcgc
     3241 aggtacggat attttgccgg tgccgcgagg tgtgtaccgc tatcatcctg cataaaaccg
     3301 gctacgtcat gcacctgcag gcccagcgga tgaccgatac catgcggcat aaacggtccg
     3361 gtgagatcgt tttcgaccat cgcttcttca ctcatatcgg tgatgatttg atgtttacgc
     3421 agcagtttgg cgatgcgctg atggaactgg atgtggtaat ccacatagct aacgcctgct
     3481 ttcatggtgg cgatcagcgc cagttgttca tcatttacgt ctttcaccag ctgtgcgtag
     3541 tcgttgtcgc tttttgccga ccaggtacga gtcaggtcag ccgcatagcc gttatattcg
     3601 gccccggcat ccagcaggaa gctgcgcatc tcttccggtg tctgatgatc cagtttggtg
     3661 taatgcagca ccgaagcgtg ttcgttaagc gccacaatgt tgctgtaagg tacgtcggta
     3721 tcacgatgac cggtcgcggt cagataggcg atgttgatat cgaactcgct catgccagaa
     3781 cggaacgctt cttctgccgc acgatgaccg ttgaccgcca ttttctgcgc ttcacgcata
     3841 caggccagtt cgtactcggt tttgaaggag cggtagtaat gcaggtagtc gatcacccct
     3901 ttcgggttga tgttgctggc ctcaataccc agttgcagcg cacgttccgg caccggaccg
     3961 atataaccga tattgccgcg cgcagcgggc agcaggctac caatgccatc ggctttcggc
     4021 agtgcgatca cttccacatc ttcagtccag aaggaggtcg gcagcggttc gacgttgtgc
     4081 cagtaatcaa ccggcagata gaaccacagt ttcggcttgt tcacgccatc caccagcaac
     4141 cagcagtttg gcacctgagt taccggcacc cacgctttga attgcgggtt cactttaaac
     4201 ggatagggat gatcgtcgag aaaaacgttg aacagctcgc cggagtgaat aagtaatgca
     4261 tccagcttga agcgcgccag cgcatcgcga gtccgttctt gtaaggtagc tatatgattt
     4321 ttatagagcg aggccagtga ttccattttt tacccttctg tttttttgac cttaagtctc
     4381 cgcatcttag cacatcgttc atccagagcg tgatttctgc cgagcgtgat cagatcggca
     4441 tttctttaat cttttatttg catattttta acacaaaata cacacttcga tgcatctggt
     4501 acgaccagat caccttgcgg attcaggaga ctgacatgct ttacaaaggc gacaccctgt
     4561 accttgactg gctggaagat ggcattgccg aactggtatt tgatgcccca ggttcagtta
     4621 ataaactcga cactgcgacc gtcgccagcc tcggcgaggc catcggcgtg ctggaacagc
     4681 aatcagatct aaaagggctg ctgctgcgtt cgaacaaagc agcctttatc gtcggtgctg
     4741 atatcaccga atttttgtcc ctgttcctcg ttcctgaaga acagttaagt cagtggctgc
     4801 actttgccaa tagcgtgttt aatcgcctgg aagatctgcc ggtgccgacc attgctgccg
     4861 tcaacggcta tgcgctgggt ggtggctgcg aatgcgtgct ggcgaccgat tatcgtctgg
     4921 cgacgccgga tctgcgcatc ggtctgccgg aaaccaaact gggcatcatg ccgggctttg
     4981 gcggttctgt acgtatgcca cgtatgctgg gcgctgacag tgcgctggaa atcattgccg
     5041 ccggtaaaga tgtcggcgcg gatcaggcgc tgaaaatcgg tctggtggat ggggtagtca
     5101 aagcagaaaa actggttgaa ggcgcaaagg cggttttacg ccaggccatt aacggcgacc
     5161 tcgactggaa agcgaaacgc cagccgaagc tggaaccgct aaaactgagc aagattgaag
     5221 ccaccatgag cttcaccatc gctaaaggaa tggtcgcgca aacggcgggg aaacattatc
     5281 cggcccccat caccgcagta aaaaccattg aagctgcggc ccgttttggt cgtgaagaag
     5341 ccttaaatct ggaaaacaaa agttttgtcc cgctggcgca taccaacgaa gctcgcgcac
     5401 tggtcggcat tttccttaac gatcaatatg taaaaggcaa agcgaagaaa ctcaccaaag
     5461 acgttgaaac cccgaaacag gctgctgtgc tgggcgcagg catcatgggt ggcggtatcg
     5521 cttaccagtc cgcgtggaaa ggcgtgccgg ttgtcatgaa agatatcaac gacaagtcgt
     5581 taaccctcag catgaccgaa gccgcgaaac tgctgaacaa gcagcttgag cgcggcaaga
     5641 tcgatggtct gaaactggct ggcgtgatct ccacgatcca cccaacgctc gactacgccg
     5701 gatttgaccg cgtggatatt gtggtagaag cggttgttga aaacccgaaa gtgaaaaaag
     5761 ccgtactggc agaaaccgaa caaaaagtac gccaggatac cgtgctggcg tctaacactt
     5821 caaccattcc tatcagcgaa ctggccaacg cgctggaacg cccggaaaac ttctgcggga
     5881 tgcacttctt taacccggtc caccgaatgc cgttggtaga aattattcgc ggcgagaaaa
     5941 gctccgacga aaccatcgcg aaagttgtcg cctgggcgag caagatgggc aagacgccga
     6001 ttgtggttaa cgactgcccc ggcttctttg ttaaccgcgt gctgttcccg tatttcgccg
     6061 gtttcagcca gctgctgcgc gacggcgcgg atttccgcaa gatcgacaaa gtgatggaaa
     6121 aacagtttgg ctggccgatg ggcccggcat atctgctgga cgttgtgggc attgataccg
     6181 cgcatcacgc tcaggctgtc atggcagcag gcttcccgca gcggatgcaa aaagattacc
     6241 gcgatgccat cgacgcgctg tttgatgcca accgctttgg tcagaagaac ggcctcggtt
     6301 tctggcgtta taaagaagac agcaaaggta agccgaagaa agaagaagac gccgccgttg
     6361 aagacctgct ggcagaagtg agccagccga agcgcgattt cagcgaagaa gagattatcg
     6421 cccgcatgat gatcccgatg gtcaacgaag tggtgcgctg tctggaagag ggcattatcg
     6481 ccactccggc ggaagcggat atggcgctgg tctacggcct gggcttccct ccgttccacg
     6541 gcggcgcgtt ccgctggctg gacaccctcg gtagcgcaaa atacctcgat atggcacagc
     6601 aatatcagca cctcggcccg ctgtatgaag tgccggaagg tctgcgtaat aaagcgcgtc
     6661 ataacgaacc gtactatcct ccggttgagc cagcccgtcc ggttggcgac ctgaaaacgg
     6721 cttaaggagt cacaatggaa caggttgtca ttgtcgatgc aattcgcacc ccgatgggcc
     6781 gttcgaaggg cggtgctttt cgtaacgtgc gtgcagaaga tctctccgct catttaatgc
     6841 gtagcctgct ggcgcgtaac ccggcgctgg aagcggcggc cctcgacgat atttactggg
     6901 gttgtgtgca gcagacgctg gagcagggtt ttaatatcgc ccgtaacgcg gcgctgctgg
     6961 cagaagtgcc gcactctgtc ccggcggtta ccgtcaatcg cttgtgtggt tcatccatgc
     7021 aggcactgca tgacgcagca cgaatgatca tgactggcga tgcgcaggca tgtctggttg
     7081 gcggcgtgga gcatatgggc catgtgccga tgagtcacgg cgtcgatttt caccccggcc
     7141 tgagccgcaa tgtcgccaaa gcggcgggca tgatgggctt aacggcagaa atgctggcgc
     7201 gtatgcacgg tatcagccgt gaaatgcagg atgcctttgc cgcgcggtca cacgcccgcg
     7261 cctgggccgc cacgcagtcg ggcgcattta aaaatgaaat catcccgacc ggtggtcacg
     7321 atgccgacgg cgtcctgaag cagtttaatt acgacgaagt gattcgcccg gaaaccaccg
     7381 tggaagccct cgccacgctg cgtccggcgt ttgatccagt aagcggtacg gtaacggcgg
     7441 gtacatcttc tgcactttct gatggtgccg cggccatgct ggtgatgagt gaaagccgcg
     7501 cccgtgaatt aggtcttaag ccgcgcgccc gtgtgcgttc gatggcggtc gttggttgtg
     7561 atccgtcgat tatgggttac ggcccggttc ctgcctcgaa gctggcgctg aaaaaagcgg
     7621 ggctttctgc cagcgatatc ggcgtgtttg agatgaacga agcctttgcc gcgcagatcc
     7681 tgccgtgcat taaagatctc gggctaatgg agcagattga cgagaagatc aacctcaatg
     7741 gtggtgcgat cgcgctgggt catccgctgg gttgttccgg tgcgcgtatc agcaccacgt
     7801 tgctgaatct gatggaacac aaagacgttc agtttggtct ggcgacgatg tgtatcggtc
     7861 tgggtcaggg tattgcgacg gtgtttgagc gggtttaacc acttgccgga tgcggcgtga
     7921 acgccttatc cggtctaccg atcctgcacc aatgtaggcc tgataagacg cgccagcgtc
     7981 gcatcaggca tcgtgcaccg attgccggat gcgacataga tctaaatgcc actgaataaa
     8041 gttttagttg ccgttcttcc cgcctgtcag gggcgggttt ttatatctca gataaatgca
     8101 aacgcatcgc caaacagacg atcttcccgc gcattacgct cactgcaaaa caggtcacga
     8161 gcaattttcg ccatctcaaa acgtccggca atatagatat catgctctgc cagcgtacca
     8221 tgatcctgca agaccgccgt taacacggtg ccagtacgcc cacgccagcc cgcttccggt
     8281 tgttcaacca ccggcaccac ttgcagacca ggatgcttca gcgaaagcgc ctcaagctcg
     8341 cagagatcat acagatgttg ctcttcacgc ccaccccagt aaatggtgat atcgcgattg
     8401 ggattacgcg ccaacgccgt caataaaatc gagcgcgcat aggagaaccc ggtgccgccc
     8461 gcaatcaaaa tcatcggacg ctcttcatca tcgcgcagcc acgcttctcc gtggggaata
     8521 tcgaccacga tttgatgatc tttgaggatg cggtccatga ctgctttcgc gtaaaggttg
     8581 atttcagaag cgccaatatg cagctcgata aacccttttt catccggcgt cgaagccatt
     8641 gaaaacggac gtttgtcgcg ctcatccatc actaccatca aatactgacc agcacgaaaa
     8701 gaaaaggccg cgtctggtac gattcggaca cggtatacag tatccgtgat agcttctacc
     8761 gaggtcactt tacagcttaa ggttgtcatg cgctttctct gtcggatcaa ataaataggg
     8821 caaaactaac gcgcatcagg cgcttttacc gttgttaaaa atagccagtt catcccagat
     8881 ggcgtcaata tgcgcgacga catcaggatc ttttttgatg ggacgtcccc attcacgctg
     8941 ggtttccccc ggccatttat tcgtggcatc cagccccatt tttgaaccca gcccggagac
     9001 aggcgaggca aaatccagat aatcaatagg cgtattttct accagaacag tatcccgcgc
     9061 cgggtccata cgggtggtaa tcgcccaaat cacatcgttc cagtcgcgtg cgttgacgtc
     9121 atcatcgcaa acgatcacaa atttagtgta cataaactgg cgtaagaacg accagacgcc
     9181 catcatgacg cgcttcgcgt gtccggcgta ctgttttttg attgtcacta ccgccagacg
     9241 ataagaacag ccttccggtg gcaggtaaaa atcgacaatt tccgggaact gtttttgcag
     9301 aatcggcaca aacacttcgt tcagtgcgac acccagcacc gcgggttcat ccggcggacg
     9361 accagtatag gtggagtggt aaatcgcatc ttcacgctgg gtaatatgcg tcacggtaaa
     9421 taccgggaaa ctatcgactt cgttatagta accggtgtgg tcgccatacg gcccttccgg
     9481 cgcagtttcg ccttgttcga tatacccttc cagcacaatc tccgcactgg cgggcacttc
     9541 aaggtcattg gagatacact tcaccacttc ggtcttggta ccgcgcagca atccggcaaa
     9601 cgcatactct gaaagtgtat ccggaacggg ggtgactgca ccgagaatcg tggcgggatc
     9661 ggcacccagc gccacagaaa ccgggaaacg ttcgcccgga tgcgccgcac accactcctg
     9721 ataatccagc gcgccgccgc gatgcgacag ccagcgcata atcagtttgt ttttaccaat
     9781 cagctgctgg cgataaatgc ccagattctg ccgctcttta tgcgggccgc gcgttacggt
     9841 cagcccccag gtaatcagcg gcgcggcatc ttccggccag caggtcataa tgggaatgcg
     9901 attgagatcg acgtcatcgc cagagacgat tttttgttgg cagggcgcac cacgcagccg
     9961 ctttgtcggc atgttcaata cttgcttaaa ctgcggcagt ttatcaaaca ggtcgcggaa
    10021 accttttggc ggctcgggct ctttcagaaa cgccaataat ttaccaactt cacgcagcgc
    10081 cgaaacatct tcctgcccca tgcccatcgc cacgcgcttt ggcgtaccga acaggttgca
    10141 cagcaccggc attgagtagc ctttagggtt ttcgaacaac agcgcaggcc caccggcacg
    10201 caaagtgcgg tcagcaattt cagtgatttc cagatgcgga tccaccggga gcgtgatacg
    10261 ttttagctca ccctgctgtt caagcagcgt caagaagtcg cgtaaatcgt tatatttcat
    10321 ggcgtccatt gtagcctctt aatctacgcc cattatacgg cgttcatctt tgcgatgctg
    10381 taaatttgtt aaattagcgt gaactctgac ggtataacgc aaaccgggga atataattaa
    10441 cttagcgtaa agcttttgct atccttgcgc cccgattaaa cggataagag tcattatgca
    10501 atcttggtat ttactgtact gcaagcgcgg gcaacttcaa cgtgcccagg aacaccttga
    10561 gagacaggct gtgaattgcc tggcaccgat gatcaccctg gaaaaaatcg tgcgtggaaa
    10621 acgtactgca gtcagtgagc cattgtttcc caactacctg tttgtcgaat ttgatccaga
    10681 agtgattcat accacgacta tcaacgcgac ccgcggtgtc agccacttcg tgcgctttgg
    10741 cgcgtcgcta gcgatagtcc catcggcggt gattcatcag ctatcggtat ataaaccgaa
    10801 agacattgtc gatccggcaa ccccttatcc gggagataag gtgattatta ccgaaggcgc
    10861 gttcgaaggc tttcaggcca ttttcaccga acccgatggt gaggctcgct ccatgctatt
    10921 gcttaatctt attaataaag agattaagca cagtgtgaag aacaccgagt tccgcaaact
    10981 ctaaaacgca atcccaaacc gtgttttgac attagcatcc gtggtggcag ccagccatgc
    11041 ggcatcttct ccacgccagt gcgcaatacg ttgcaaaata tggggcagat gggctggctc
    11101 gttgcgccgg gatgatggct ttggcgtgag atcgcgaggg agcagatacg gcgcatcagt
    11161 ttcgatcagt aatttttccg ccggaatcaa cggcaaaagt tcccgcagct ccagtccgcg
    11221 tcgttcatcg taaacccaac cggtaatgcc gatataaatt ccatgcgcca cgcacgcctg
    11281 catctcttcg cgtgtgccgg taaagcaatg aagaaccgca ccaggcagtt tatccagcca
    11341 cggctccagc aatgtcataa accgctcgtg ggcatcgcga cagtgcataa ataccggcat
    11401 gtttaaatct gcggcaatgc gtagctgggc aacaaaagcg cgttcctgct cttccggcgt
    11461 cgaaaagttg cggttaaagt cgagaccaca ttcaccaatc gccaccactt ctggctgcgc
    11521 ggccagctca ataatcgctt cttcagtcgc agcttgccac tggctgctgt cgtgaggatg
    11581 tacgcccgcc gttgaccaac aggacgaata ctgacgcgcc agcttttgcg cctgctggct
    11641 ttcacgcagg ttagtgccgg tgatgagtag cccattaact cccgcgtcaa aagcgcgcgc
    11701 tacaacatca tcacggtctt tcgcaaattg cgaactggtc aaattaacgc cgatatcaaa
    11761 catcctgtac tccatatgac aaccgccctg acgggcggtt gaatttattc ttcagttttt
    11821 tcgctttctg cttcagcgtc gttttcctct tcccgatttc gccctttacc aacgtaaaag
    11881 cgtgagaaga agacaccgat ttcaaacaga cagtacatcg ggatcgccaa tagcgtttgc
    11941 gagaagacat ccggcggcgt cagcaacatc ccgacaacga atgcaccaac cagcacatac
    12001 gggcgttttt tgcgtaagtc ttctggcgag gtaatcccca tccagcacag cagcacaatt
    12061 gccaccggca cttcaaagga gacaccaaac gccataaaca gcgccataac gaagcttaaa
    12121 tagctggcga tgtcggtgga tacctgcacc ccttccggcg cggtattggc aaggaagcca
    12181 aatgccagcg gaaagaccac aaagtaggcg aatgccatgc cgatataaaa cagcagagag
    12241 ctggaaacca gcagcggcac caccaggcga cgttcatgct tatacagcgc tggggcgata
    12301 aatgcccaca cctgatagag aatcaccggc gctgacagaa tcagcgacac cataaaggtc
    12361 agcttgatcg gcgtaaagaa cggcgaggcc acgtcggtgg cgatcatcgt tgaaccttgc
    12421 ggcaactgct tgatcaatgg cgcggatacc aggtgataga tgtcattggc gaaatagacc
    12481 agacacagga atatcacgat caccgcgata atgcagttca gcagacgctt acgcagctca
    12541 attagatgcg tgataagcgg ttgagtatct tctacagaca tgtttacggt ttatcactcg
    12601 acgaagggga aggtgcagcg gttttcggtt cagcgtccgc agcaggtttt accaccggct
    12661 ctggcgtggt ttctggcttc tgttccggcg aactggcctg cgtttgtgca gcggcaggcg
    12721 ttacgccctc atgcgcagct tcattatctt tcaccaccgg gttatggatg gtgtgcgctt
    12781 catcgctcgc cttttcagga tcgtttgcaa cgtaggaacg cttcatcgac tccgcggcct
    12841 ggcgtagttc atccatcgac gctttcagtt cgggcgtcag gttagtgagg ctcgcctttt
    12901 caaccttttt cagactgtcc tgaaactcct ggagttttaa ctcctgggtc agttcgttct
    12961 gcaccgttgt cgccagtgaa cgcaacgcgc gaatccagcc cgctaccgtt tttaccgcca
    13021 caggcagtcg ttgcggcccc agaacgacga ggccgatgat gaacaccaat agcagttcgc
    13081 taaaaccgat atcaaacacg gattacacct gctctttatc gtggcgcttc gcgtcttctg
    13141 ttttagcctg ttcctgattc gtatccgcct gcttatcggc gatagttttc gcagtaaaat
    13201 cagcatcctg actggtttta tcctgctttg gttcatcatc gctcattgct tttttaaagc
    13261 ctttgatcga cgcaccaaga tcggaaccga tggagccgag ctttttggtg ccaaaaagca
    13321 gtacaacgat gacggcaata atcaataact gccaaatact gataccaccc atacatgttc
    13381 ctctgtggta gatgatgatt aaacaaggcc gcattatacg ttacacgacc cgccttgagc
    13441 gatgaaaaaa tcagcgtgtt ttgcgccaac cgacaaacca ggcgatcaga ccacctgcca
    13501 ttaaccagcc gggcatcagc ccccattcag gtcggctgac caacaagaat gtgccactta
    13561 atactaacgt agcgccaatt ccgagaaaat aacgcgattg tccctgacgt acatgatttg
    13621 actgaagctc gcgggcaatc ttatcaacac tgtgctgtaa atacttgccc tggcgcaaac
    13681 tgtcgtaaac caattcaggc agttctggca ttttttcgac ccagaacggc gctttttctt
    13741 taaatgctct caccagggca ggaataccga cctgatcttt aatccacgac tccaggaaag
    13801 gcttcgctgt tttccataaa tcgagctgcg gataaagctg gcgtcctacc ccttcgacat
    13861 agagcagggt tttctggagt aacaccagtt gcggctgcac ttccatattg aagcgacgcg
    13921 ccgtattaaa cagatttaac agtacatgtc caaacgaaat ttcggccagc ggtttctcaa
    13981 agataggttc acagaccgta cgaatggcaa attcgaactc ttcaacgttg gtatctggtg
    14041 gcacccagcc agaatcgacg tgtagctctg ccactttgcg atagtcgcga ttaaagaagg
    14101 cgataaagtt ttctgccaga tagcgtttat cttctttgtt tagcgagcca acaatcccgc
    14161 aatcaatgcc gatatatttc gggttttccg ggtgttcata gcttacgaaa atgttgccag
    14221 ggtgcatatc ggcatggaaa aagctgtcgc gaaagacctg agtgaagaac acctgcacgc
    14281 cacgttccgc cagcaatttc atgttggtgc catttttctc cagcgtcgca acatcagaca
    14341 ccggaatgcc gtaaatgcgc tccatcacca tcatcccttc actacaatag tcagggtaaa
    14401 cttccgggat gtagagcatc gggctgtctt caaaatttcg ccgaagctga atggcgttgg
    14461 cagattcccg cagcaaattc agttcatcaa tcaaggtctt ttcgtactcg cgcaccactt
    14521 cggttgggcg cagacggcga ccatccggca gcaaacgcgg cacccagcga gccagacggt
    14581 agataagttt cagatccgct ttaataaccg gcaaaatatc cgggcggatg actttaatca
    14641 ccacctcttt accattcgat ttcaatcgcg cggtatgaac ctgggcgata gaagcagaag
    14701 ccagtggctt gatttcaaaa tcgtcaaacc acgcttctac cggcaagccg cccattgcag
    14761 cttcaatctg ctgcttcgcc agcttgccat caaacggagc aactttgtcc tgcaataacg
    14821 ccagctgatc ggcaatatgc ggtggaaaaa gatcgcggcg ggttgataac atttgcccga
    14881 acttgatcca aaccggcccc agttcttgca gggccagtcg tagtcgctca cctaaaagtt
    14941 tgtctttatg ccgatttggc atccagaata atgagtatcg ccatagccgt agcggcaggg
    15001 tgatacgcat tttggggatc agttcatcaa gtccgtagct taaaaaagtg cgaatgatga
    15061 aatataggcg ccgtacttca cctggcgtca tttagcctcc agtttttcca gccgtttggt
    15121 cagggcatca acagcacgct cgacggcagc cgtctcttcc gcaaaccagg ccacttcaag
    15181 cggaccgggt gccatacgcc actcttcagt aatggcttcc gccacataac gttgctggcg
    15241 cttaatgccg tgatgcagga actttgcgcc tccgcgcatg gctttgctga ttccttcagc
    15301 ggcgatatca ccggtataag gggccagcag ttccgcaggg tcgaactctg ccagatctgc
    15361 cagcgcaacg aagttttgca ccacctgaat atcgccctgc acttccagct caccactgcg
    15421 aatcagtgcg gtaagctgct ggcgatcgcg aagtttcggc aacacactgg cgtaggcgat
    15481 aacggtgcag tcagcatcgc ctgcccattc gcccagtaca tcaacctggc gttcgctgaa
    15541 caccagaatc aatgacgtcg aaaagccttt tacctccacg cgcaatactt tacccagcag
    15601 acgcgagcgg gctgttttca gcgcgggtga gcgatacagg aaggtgttga gcagactttc
    15661 aattcctgcc gtcactaaag gtttaaaagg catttccggt ctcctgtcag aacttataac
    15721 cacgatgcag cgccacaacc cctgccgtca gattgtagta gtcgacactt tcgaatccgg
    15781 catcctgcat catggctttc agggtatcct gatcgggatg catacggatg gattctgcca
    15841 gataacggta gctgtcggcg tcgttcgcga ccagtgagcc aatacgcggc agcacatgga
    15901 aggagtatgc atcataggct ttgctcagcg gctcgataat tggcttcgag aactcaagca
    15961 ccagcaggcg gccgccgggt ttcagcacgc gatacattga acgcagtgct ttatctttgt
    16021 cggtgacgtt acgcagacca aacgaaatgg tgatgcaatc aaaggtgtta tccgggaacg
    16081 gcagcgcctc agcgttcgcc tgaacatact caacgttgcc aatcacaccg atattacgca
    16141 gcttctcgcg tcccattttg agcatggatt cattgatatc agcaaggacc actttgccag
    16201 tttctccgac caggcgggag aatttcgctg tcaggtcgcc ggtgccacca gccagatcca
    16261 gcacggtctg cccacggcgt acgccgctgc aatcaatcgt gaatcgcttc cacaaacgat
    16321 gaataccaaa tgacatcaaa tcattcatga catcgtattt tgatgccacg gaatggaaaa
    16381 cgtgggcgac catatccgct ttttgttcct tcgcgacggt ctgaaaacca aagtgcgtcg
    16441 tttcttgtga cttatccacc atctcaatgc ctgctcatca aaaaattgtt ccagaagtgt
    16501 aacagattgg gcgtcgatgc cctagatttc tacccggctt aactacccct aatgggctag
    16561 cgcgactgct gattatattc atcatcgcgt tgataagctt catcattcgg ctgctccgga
    16621 accgaccgaa gtcgatactc ttcattctgg ctcaccgcct gttcagccaa atccggatta
    16681 atctcgcgtt taatttctac tcctaaaccg cgaaacgctt ctgcctgcgc cagcacattt
    16741 ccgcgacctg aagagagttt tttcattgcc tggcggtagt tatcctgcgc tttgtcgaga
    16801 ctttgaccaa tcgcggacat gtcatcgatg aacaaacgca tcttgtcgta cagcttgctg
    16861 gcacgatcgg cgatttgctg ggcgttgcgg ctttgatgct cataacgcca caggttggca
    16921 atagtacgca acgccaccag cagcgtagtc gggctaacca gcatgatatt gtttttcaac
    16981 gcttcggtga tcagctccgg ctggcggtca agcgccagta aaaaagcggg ttcaacggga
    17041 ataaacatca gcacgtaatc cagagttcgc agccccggca gctgttgata atctttgcgt
    17101 cccagcaaac ggatatggtt acgcaccgac gcgatatgtt cctgtagcgc gctttcgcgg
    17161 gtgtagtcgt cttcggcgtt aaaatagcgt tcataggcga ccagcgtcat tttggcgtcg
    17221 atcaccacat cttttccctg cggcagccgc acgatgacat ccggctgcat ccgcgagcgg
    17281 gcgtcatttt cgatgctgac ctgggtttca tattcatacc cttcacgcag accggaagcc
    17341 tccagcaccc gcgtcaatac tacctcgccc cagttgccct gggttttatt gtcgcctttc
    17401 agcgcgcgcg tcaggttgat cgcttcctgg gccatttgcg cgttgagttg ctggagattg
    17461 cgaatttcgt gggtcagggt atggcgttct tgtgcttctt taccgaagct gtcctgaacc
    17521 tgacggcgga aaccgtccag ttgttcacgt agcggcgaca acaggctgtt cagactctga
    17581 cggttttgct catcaacccg gcgattgctg tgctcaaaaa tacggttggc gaggttttca
    17641 aactgctcac tgaggcgctg ctcgctgtta atcatctggc gaattttatc gtcagcatgt
    17701 tgctgtgcgg cttccatccg cgtggttact tcacgcagat cggcctccag agaggtgtta
    17761 atactttgca ggctgcgcac ttcgttattg agtaactcgc actctgcacg ccagtgctcg
    17821 ctttgggtaa tttgttgttt tgccgcgctt aactccgcga ccatctcttc acgttcagct
    17881 aattgctcgg ctttttgctg cgcatgttga taactggcaa acagccagcc aattgccaca
    17941 cccaccaacg caataactgc gtaaaccatg attgagaaat ccacaacgcc tcctgacgca
    18001 acaacttacc cgcacaaaat agaattgtgc tgtacaaacg tccagttgga aaggcatttc
    18061 ctgcgaggcg ctacgcaaaa atacaaaagg ccgaacgcgt cggccttcag acaggagaag
    18121 agaattacag cagacgacgc gccgcttcca ccacgatttt caccgcttgg ctttcagttt
    18181 gtttcatcgt ctcagcattc gggatctctt gctgggtgcg gttaacgata acacccgcta
    18241 ccataccggc acgcaggccc tgacttgcac acatggtcag cagggttgca gattccattt
    18301 catagttcat tatgcccatc gcctgccact cttccataga acctttaaag tgacgaacta
    18361 cgcgaccaga gtaagtatcg taacgttcct gacctgggta gaaggtatca gaagaagctg
    18421 tcacgccaac gtgagttgtc gcgccaatgg atttcgcagc ttcaaccagc gcagtcgtac
    18481 attcgaaatc agcgacagcc gggaattcca gcggtgcgaa gtgcaggctc gcgccatcca
    18541 gacggacaga cgccgtggta accaggacat cacccacatt aatatgcggc tgaatagcgc
    18601 ccgttgtacc gatacgcagg aaggtgcgaa tgcccagctg tgccagctct tcaacagcaa
    18661 tagaggtaga cgggccgccg ataccggtag agcagacgat aacaggttta ccatccagct
    18721 ctgcacgcca ggtagtgaat tcgcggtgag atgccagctt aaccggctta tccatcagcg
    18781 cggcgatctt ttccacacga tccgggtcgc cagggacgat ggcaagcgta gccccttgta
    18841 aatcgttttt agtgaggccg agatgaaaaa catcagactt ggacatatac aactcctctg
    18901 tgaatcggtt tagtcagaag aagcaaaaag acactttacc gaagggttta acattttttc
    18961 gtgatactca tcaccatgac gcaaatgcgt tgcataaatt tacccaccaa aagtgattca
    19021 aatcacataa atcactcttc attctgcagg actgcataaa agatgagccg attcaggcaa
    19081 tgtgatttgt tgcatgctga ctatagttaa cattgcgcaa catttttata agggtacgga
    19141 gaataccatg gcaacaacac aacaatctgg atttgcacct gctgcatcgc ctctcgcttc
    19201 gaccatcgtt cagaccctgg acgacgcgat tgtggcgggc ttcacctcta tcccttcaca
    19261 aggggataac atgcctgctt accatgccag accaaagcaa agcgatggcc cactgccagt
    19321 ggtcattgta gtgcaggaaa tttttggcgt gcatgaacat atccgcgaca tttgtcgccg
    19381 tctggcgctg gaggggtatc tggctatcgc acctgaactt tacttccgcg aaggcgatcc
    19441 gaatgatttt gccgatatcc ccacgttgct tagcggtctg gtagcaaaag tgcctgactc
    19501 gcaggtgctg gccgatctcg atcatgccgc cagttgggct tcccgcaacg gcggcgatgt
    19561 tcatcgttta atgatcaccg gattctgctg gggtggacgt atcacctggc tgtatgccgc
    19621 gcataatcca cagctaaaag ctgcagtggc gtggtacggc aaactgacgg gcgacaagtc
    19681 gctgaattca ccgaaacaac ctgttgatat cgcaaccgat cttaacgcgc cgattctcgg
    19741 cttatatggc ggtcaggata acagcattcc gcaggagagc gttgaaacca tgcgccaggc
    19801 gctgcgggct gctaatgcga aagcagagat tatcgtgtac ccggatgccg ggcatgcatt
    19861 caacgctgat tatcgcccga gctatcatgc cgaatctgcg aaagatggct ggcagcgtat
    19921 gctggaatgg tttaagcagt atggagagaa gcggagttag tttcctctta gtctatattt
    19981 gagatggtga gtaaacgagt gcgttttgct gccatctctg tcccccaaaa aataatcagt
    20041 atgaattgat tggagattac gactccgcta tttgacctta agaaagtcgg caagtcatag
    20101 tcataaatga gtctcttcaa tggaagacac cttagagatt taccatcaac tcccattcac
    20161 aaacaaacat taattattat caacacattc acattaatta taaatcatct cagaccttcc
    20221 tgataaaaag atattaaagt gaaacatttt gtttttcaca ttttagttca tcacttttag
    20281 gcgtaaataa aggtaaaaaa aacaacaata ctatttctct ttcacaaaag aattaacaaa
    20341 aggtttttca aggatgagtg atattattta cctgaccata accggagaac aacaaggatg
    20401 tatttcctct cgttgtggaa cgagcgcatc aataggtaat cgctggcaga ttggccatga
    20461 tgatgaaatc ttcgcgttct cgctctctaa tagtattacg aatacaggca aagggtcata
    20521 attgcatggc cttagtttct gtaaattgat tgacaaaagc tccccgttac ttattaacgc
    20581 tattaacaat aacgagcagt tgttcatgga gtttgacttc tatcggatca ataggtttgg
    20641 tcgatgggag aaatattaca acattcaact ccgcggcgcg ctcttatcag caatcaatca
    20701 cctgtttact gagaacaatc tggatacaga agccatcact gtcagttatt aatatatact
    20761 ctccaggcat ctgattgcta atactgaatt tagttattgg gcatttcccg ataactacaa
    20821 ccgtctgttt ataccacggc caaaaactaa agctggtaat ggtttcaaaa cacttaatag
    20881 taaagcggtt ggcagactgc ttgctgctgt cggcatatac aatgggaata tcgaaggatt
    20941 cagggaaaca gcagacaagt tggggggaga tgcacctgct ggttatgatc aatttatgga
    21001 taacaaaggg ttactcatcg ctggtgcttc aatagttgct ggagcagttc tggggagaat
    21061 gagactccct gatttggaat cacttgagca tttcggagcc agaggagcag tatcaggaag
    21121 accatttaat cccgagcttg ccggtggtcc aatagaaaac ctgacgacag atggcgtaac
    21181 aatcaacagg gaaggtatcg ctattgttga aaaacatata gccagatttg gtcatgatcc
    21241 tgtaaatgaa gtgatgatca atagactgaa agacattgag aaaggaaaaa tccccccaga
    21301 acaagtagat cttaacttct atactcatga gtgtcgggaa tatcagcgtt actgtaatct
    21361 tggttgggaa acaggacaac ctgatggtga cgcaggctat gcactctgga atcatactgg
    21421 atttgcccct atatttccag acatctgtta tcacttaacc cattacaagc ccgctgcc
//
LOCUS       DAZPLI010000063        21314 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZPLI010000063 DAZPLI010000000
VERSION     DAZPLI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21314
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.064"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            263..763
                     /gene="tssB"
                     /locus_tag="ACHUU3_002237"
     CDS             263..763
                     /gene="tssB"
                     /locus_tag="ACHUU3_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFR2082289.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            798..1022
                     /locus_tag="ACHUU3_002238"
     CDS             798..1022
                     /locus_tag="ACHUU3_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2082290.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1073..2548
                     /gene="tssC"
                     /locus_tag="ACHUU3_002239"
                     /pseudo
     CDS             1073..2548
                     /gene="tssC"
                     /locus_tag="ACHUU3_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            2555..2968
                     /gene="tssE"
                     /locus_tag="ACHUU3_002240"
     CDS             2555..2968
                     /gene="tssE"
                     /locus_tag="ACHUU3_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR2082291.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2972..4822
                     /gene="tssF"
                     /locus_tag="ACHUU3_002241"
     CDS             2972..4822
                     /gene="tssF"
                     /locus_tag="ACHUU3_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR2082292.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4786..5868
                     /gene="tssG"
                     /locus_tag="ACHUU3_002242"
     CDS             4786..5868
                     /gene="tssG"
                     /locus_tag="ACHUU3_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFR2082293.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5893..7173
                     /gene="tagH"
                     /locus_tag="ACHUU3_002243"
     CDS             5893..7173
                     /gene="tagH"
                     /locus_tag="ACHUU3_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFR2082294.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7170..7694
                     /gene="tssJ"
                     /locus_tag="ACHUU3_002244"
     CDS             7170..7694
                     /gene="tssJ"
                     /locus_tag="ACHUU3_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFR2082295.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7697..9028
                     /gene="tssK"
                     /locus_tag="ACHUU3_002245"
     CDS             7697..9028
                     /gene="tssK"
                     /locus_tag="ACHUU3_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFR2082296.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHHARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKIFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPCIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9033..9794
                     /gene="icmH"
                     /locus_tag="ACHUU3_002246"
     CDS             9033..9794
                     /gene="icmH"
                     /locus_tag="ACHUU3_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFR2082297.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9803..12598
                     /gene="tssH"
                     /locus_tag="ACHUU3_002247"
                     /gene_synonym="clpV"
     CDS             9803..12598
                     /gene="tssH"
                     /locus_tag="ACHUU3_002247"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFR2082298.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESESESEQDNTGAEPADEADREQPEETAETVSPVQ
                     RLAQLTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWL
                     GDTIKGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYG
                     GRQYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKA
                     HPDVLNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLY
                     PVLADFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGVEVVIEPEVT
                     DEIMSRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTAD
                     VEDAQNDESVTKDETVL"
     gene            12595..13338
                     /gene="vasI"
                     /locus_tag="ACHUU3_002248"
     CDS             12595..13338
                     /gene="vasI"
                     /locus_tag="ACHUU3_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFR2082299.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13343..14755
                     /gene="tssA"
                     /locus_tag="ACHUU3_002249"
     CDS             13343..14755
                     /gene="tssA"
                     /locus_tag="ACHUU3_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFR2082300.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGQLAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14864..18298
                     /gene="tssM"
                     /locus_tag="ACHUU3_002250"
                     /pseudo
     CDS             14864..18298
                     /gene="tssM"
                     /locus_tag="ACHUU3_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            18309..19758
                     /locus_tag="ACHUU3_002251"
                     /pseudo
     CDS             18309..19758
                     /locus_tag="ACHUU3_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19685..20167
                     /locus_tag="ACHUU3_002252"
     CDS             19685..20167
                     /locus_tag="ACHUU3_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR2082301.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            20211..21138
                     /locus_tag="ACHUU3_002253"
                     /pseudo
     CDS             20211..21138
                     /locus_tag="ACHUU3_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21148..>21314
                     /gene="ykfM"
                     /locus_tag="ACHUU3_002254"
     CDS             21148..>21314
                     /gene="ykfM"
                     /locus_tag="ACHUU3_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkfM"
                     /protein_id="HFR2082302.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLIL"
BASE COUNT         5122 a         5722 c         5798 g         4672 t
ORIGIN      
        1 aaactctata tttaaattat tgccataata cacctgacgc gaattgcaca ggttcacaca
       61 atctatttaa cgcttgcaga atacccctct ttacgatcga taaaaagtcc ttttcttatt
      121 tattttgaag cgtaaaatgc ggggtgatga aggagtggtg attttatttt caccgccatt
      181 agggcggcat tgccgatatt tttttgcggt acaaatatgc cgtgttcatg gcctgtacga
      241 gataacatga cggatcactt ttatgagcaa aaaatttgaa ggcagcgtcg cgccccgcga
      301 gcgtattaat atatcgtatg tcccaaaaac agatggccag acggctgaag tggaacttcc
      361 gcttaacatg ctcgttgtcg gcgataccgg aaatacgcag gaaacatccc cactggatga
      421 acgtcaggca gtttccgtta acaagcacaa tttcggtgca gtgatggctg aagcagccat
      481 tggcctcaat tttactgtcc ccgccacgct gaagggaagc acaacagacg atgaactgaa
      541 tgtggcgctg aatatcaaat cactggatga tttttcacca gatagcgtgg cccggcaggt
      601 tccggaagtg aataaattgc tggaactgcg tgaagcactg actgcactga aggggccgat
      661 gggtaacctt ccggcattcc gtacccagct ccaggcattg ctggaaaatg aagagtcccg
      721 cgagcagttg ctcaaagaaa tcggtctggt gagcaataaa taaccaccac tatttcatta
      781 aacagggtta tccaggcatg acggcacaca aaaatatctc cgggacatac cacctctctg
      841 ttgcagacat tctgcaggtg gtttaccagg tatgtttcag cccgtccgtt gaaatcaacc
      901 aggatggtgt ggctgcgctg attacaacac tggatcgccg tatcagtgat ctattggatg
      961 agattatcca tttttgtgag tttcagcaga gcgcgtcgca ctggcagcgc gtacttcact
     1021 gatggcgaaa agccggtttc agaaacacag ctaaaacaga aggattttca gtatgtcttt
     1081 acaggaagag gaactcgttt ccagtcatgc cgggcagcct gagcaggcat cgtcgctgct
     1141 tgatcaaatc atggcacaga cccgtattca gcccggttcg gaaggttatg acgttgccct
     1201 ccagggtgtg acagccttta ttgccagcat cctgcaatcc actgcatctg ccgaaccggt
     1261 caataaactg gccgtggaca gcatgattgc agatattgat gaacgcatca gccgccagat
     1321 ggatgtcatc atccatgcgc ctgcctttca gcaggtcgag tcgttctggc gctcactgaa
     1381 aaccatggtg gatcgcgttg atttccgtga aaacatcaag gtcaacgtgc ttcatgtcac
     1441 caagcaggaa cttctggaag actttgagtt tgcgccagaa attattcagt ccggatttta
     1501 taagcacgtt tattcgtccg gtttcggaca gtttggcggt aaacccatcg ctgccgtgct
     1561 gggcgcttat gagttcaaaa ataccgcgcc tgacatgaaa ctgctgcaat acgtcagtgc
     1621 cgtgggcgca atggcgcacg caccgttcct gtcctccgtt tccccggagt ttatggggct
     1681 gaactcgtgg accgaactgc ctaatatcaa agatctttat gccatctttg aaggtccggc
     1741 ctacaccaaa tggcgcgccc tgcgtgactc ggaagattcg cgctatttag ggcttacggc
     1801 accccgcttc ctgcttcgcc agccctattc accaacagat aaccctgtta agaactttaa
     1861 ctattacgaa gatgtcagcc agaaccacga agattatctg tggggtaata cggcctggat
     1921 gctggcatgc aatatcgcag acagttttgc caaataccgc tgatgtccaa acattatcgg
     1981 tccgcaaagc ggcggcgcag tgaaagatct gccgatgcat ctgttcgaaa cgatggggca
     2041 gattcaggcc aaaatcccaa ccgaggtgct ggtcaccgat cgccgcgaat ttgaactggc
     2101 tgaagagggt tttatcaccc ttaccatgcg taaagactct gataatgcag cctttttctc
     2161 tgcaaactcg gtacaaaaac cgaagcactt tccgggaaaa gatgcagaaa ccaactataa
     2221 gctgggtacg cagcttcctt atctcttcat catcaatcgg ttagcgcact acatcaaggt
     2281 gttgcagcgt gaacaactgg ggtcatggaa agaaaggagc gacttagaaa gagagcttaa
     2341 tacctggatc cgtcagtatg ttgccgacca ggaaaacccg cctgcagacg tgcgcagccg
     2401 caaacctctg cgcgctgcca aagttgaggt tatggatgta gaaggcgaac ccggctggta
     2461 ccaggttgcg ctaagcgtga gacctcattt taaatttatg ggggcaaatt ttgagctttc
     2521 cctggttggc cggttagaca gggagtaaca caccatgata ccggatcggg aaagaaccac
     2581 aggttctctt tttgagcgta tggaagcctc ttcggccaga aaccggcagg gaggcagtat
     2641 tcattcgctc aggcaatcca ttcgccagaa cctacgaaac attcttaata cccggtccgg
     2701 tagttgccgg ggtgcgcctg agctgggcat tgatgaaccg gaaggggctg aaaacttccg
     2761 cgaatccatg agtcgggcca tcgagcaatg tatagaacgc tatgaacccc ggatctcaca
     2821 cgctgaagtg caggcagtgg tttcatccgc ctccagcccg ctggatatga cgtttcatat
     2881 tacagcctgg gtgacattta acgaaacgca tgaggtgctt gaatttgata tggcaccgaa
     2941 tggcagccag cattaccggg tggactgaca aatggaattt gaagaacgct atttccggga
     3001 agaactcgat tacctgcgcc agcttagcaa gctgctggca acggaaaaac cccatctggc
     3061 ccgcttcctg gccgaaaaag atgcagatcc ggatattgaa cgcctgctgg aaggggtggc
     3121 ttttcttacc ggcaatctcc gccagaaaat tgaggatgaa ttcccagaac tgacgcacgg
     3181 gcttattaag atgctatggc ctaattacct gcgtccggtt ccggcaatga cccttattga
     3241 atatacgccg gatatggata agtcttctgt accggtgtta atcccccgta atgagcagtt
     3301 tacaaccaac gccggggaaa tcagagttga tgaagtgctg ccctctgatg ctaaaaagga
     3361 ggagccgcct ccctgtacct tcaccctctg ccgggatatc tggctgctgc ccgttcgcct
     3421 ggagcagatt gaaaaccgca gtacgacccg taatggtgtt atcaacatca ccttttcggt
     3481 cgcaccggga acggacttcc gcacgctgga tctgaacaaa cttcgcttct ggctcggcaa
     3541 tgacgacaac tatacccgtg accagcttta tttatggttc tgcgaatact tgcagggtgc
     3601 cgacctgact gtgggtgaac agcatattcg cctgcctgag tttatgctaa aagctgtcgg
     3661 ttttgagccg caggatgcca tgctgccctg gccgaaaaac gtccacagcg gctaccggat
     3721 ccttcaggag tatttctgtt accccgatgc gtttctcttt tttgatcttt gtggttgtcc
     3781 ggctttgcct gacggattgc aggcggaatt ctttaccctg caactgcgtt tttcgcgccc
     3841 tttgcccgtg gacatccggc tgcgccgcga ttccctgcgc ctgtattgcg cacctgccat
     3901 taatttattt atccaccatg cagaagccat cacgctggac aaccggcggg cagactatcc
     3961 gctggttccc agccgccatt acccacaaca ttacgatgta ttttccgtta acagtgtggt
     4021 gagccaggtc caggatatgt tcaggaaaaa agatctgggg cgtcctgttt cgacgcaggc
     4081 cgcgcgccag tggccagcct ttgaaagttt cagccatcag atagaataca gccggaagcg
     4141 ggaagtggtg tactggcatc accggaccaa aacatccctg ttccatcgcg gctttgatca
     4201 tacccttgcc tttatacatg ctgatggcag ttatccgtca gacgaatctc tgctcagtaa
     4261 tgaagtggtt tcggtatcgc tgacctgtac caaccgtgag cttccgtcac aaattcgttc
     4321 cggcgatatc accggcacaa ccggaaaaaa tgcggctgtt gcttcatttc gcaacattac
     4381 ccgcccgacg caaccactct ggccggtcat tgatggcagc ctgcactggc ccctactctc
     4441 cgccatgaac ctgaattatc tgtcattact ggatacggac gcgctgaagc aggtcatcgc
     4501 caactttgat cgccacgcaa tccatcatcc gcagacggca cggctgtcac aacaaaagct
     4561 ggatgccatt gagcgtctgg agacccgccc cgttgatcgc ctgtttacgg gtattcccgt
     4621 ccgggggctg gcttccacgc tgtatctgca cccggagccg tttgtctgtg aaggggaaat
     4681 gtatctgctc ggtacggtgc tttcgcattt tctgtcgctg tacgccagcg ttaactcatt
     4741 ccacatgctg accgttgtga acacagaaag ccaggagaca tggaaatgga cggaaagaat
     4801 cgggcagcat cctcttatct gagtccggga aatcccccgg cagataaaga gcagaatgat
     4861 ccgctggcac aggttttcca taatgcctgc tcttacaatt tttttgcgat ggcggagctg
     4921 ttgcaccgcc tggcaaaggg tgaaaagggg acgccagaat tatccctgcg cgacgatccg
     4981 gcacaggaaa ccctgcgttt cagcgccgat gccagccttg ccttcccgtg cagtgatatc
     5041 agtgcgctga aacgggatac gtcaggggca ttcaggatga ccaccacctt tatggggtta
     5101 caggggagcc agtcgcctct ccccggttat tacctcgctc acctggcctg gaaagcggta
     5161 catgaacaaa gtccggtagg tgattttctg gatatgttca gccaccgcct gacccagttt
     5221 gtctggcata tctggcgtaa gtaccgctat cacatcagtt ttcgtaacgg cggtgtggat
     5281 gcgttctcgc agcgcatgta ctcccttgtc gggctgggcc accgccagct tcgcgataag
     5341 ctggcgatta atcacagcaa aatgctggcg tattccggga tcctggcaaa tccgggacga
     5401 tccccggaaa ttatctgtgg cctggtgtcc cactgctttg atttgtcaga agtgacactg
     5461 caaaactggc aacgtcgcaa ggtggatatt gaaccggatc agcaaaatag tctggggagc
     5521 tattctctta aaaatggcga aaaactggcg ggaagatcgg tactgggcaa ctttgttctg
     5581 ggtacacgag tgcccgatct cagcggaaaa ttccagctca gtattaccag cctgacccga
     5641 aaacagttcc tctcttttct gccgtccggc gaaaacttcc tgccactgac gatgttcgtg
     5701 tccttcatcc tgcgcgatca gcttgcctgg gacttacatc tggggctggc gccggagcag
     5761 gtgggcgcaa tgcgcctggg cgataacaaa agtgcgctgc tgggctggac cagcttcctc
     5821 ggcacaccgg aagaacgacc ctcagtcacc atcagggtac ggtcgtaaat tatgataatc
     5881 acggagcaga atatgccgga agagaaacta cagacgcttt cattgcaggt cattaacggc
     5941 agtgagctgg aaagcgggcg ggcggcgcgc tgtctgttca cacagcaggg aaatgtcggc
     6001 catggccccg aatgccactg gtcggtacag gatcgtcagc agagcattcc ggcccaggct
     6061 tttaccgtta tcctgcacga tggcacattt tgtctgcgcc cgcagacggc acaactgtgg
     6121 ctgaatcagg caaaagtcac agcaacatca gacctaatac agttgcgcca gggcgatgag
     6181 atccagatcg gacggctgat ggtgagggtt catctgaacc ggggagatat tccccattac
     6241 gatgaggaaa tggccactcc cgaaaccatc gttaccaatc gcgatatgct cacggatacc
     6301 ctgctatcaa cggagggtgc gccacactat ccgggaatga ctcaccggca ccagcttgca
     6361 gacaccgtgg taaacggttt ttctgccgat ccactccagg ctcttcagtc cgaaagcctg
     6421 attaccacgg gcgatccgct ttcaggcatt gcggctgtcc ggccatcggc accgctgtcc
     6481 gatccggcaa gtaatggggg gatcaatact ccgtttatgg atctgccgcc catttatgcc
     6541 agccccggcg atcgtaatga cgacgtctct gcggcagaaa tggcgcaacg ccacgttgcg
     6601 gtcaccccct tactgcgcgg tcttggcggc tcgcttacca tgagcaattc cgacgatgcg
     6661 gatgattttc tggaggaggc cggacgaacg ttacaggccg caataaaagg tctgctcgat
     6721 ttgcagcagc agcgtaacag cctctcagac aaacatttgc gcccgctgga agataacccg
     6781 ctgcgcctga acatggatta cgccaccgcg ctcgacgtaa tgtttgccga aggtaaaagc
     6841 ccggtacatc tggcggctcc cgccgccgtc agtgaaagcc tgcgcaatgt ccgccaccac
     6901 gaagaagcta accgggcagc gattgtggag tcgcttcgtg tcctgctgga tgctttctca
     6961 ccacaaaatc tgctgcgccg ctttgtgcag taccgccgca gccatgaact gcgccagccg
     7021 ctggatgatg ccggagcatg gcaaatgtac agccattatt acgaagaact ggcctccgat
     7081 cgccagcagg ggtttgagat gctgtttaac gaggtctacg cccaggtcta tgaccgggtg
     7141 cttcgtgaaa aacagcggga gccggaagca tgaacaggaa ggcgtttctg gcctgcgtgc
     7201 tgatgtgcgt catattaacc ggctgcgaaa cggcgaaaaa aatcagtgag gtgatcaaaa
     7261 acccggatat ccaggtcggg agcctgaagg agcaaccttc ggaaataacc gtcaccctgc
     7321 tgaccgaacc ggacaccaat accaatgcgg aaggtgaatc agccgccgtg gatgtgcaac
     7381 tggtgtacct gactgacgac tcaaaattgc tggcagccga ctatgaccag atagccagca
     7441 cgccgctacc ggatgtgctg ggtaaaaact atatcgacca ccaggatttc aacctgttgc
     7501 cggacaccat caaaaccctg ccgccggtaa agctggatga gaaaacgcag tttattggtg
     7561 tcgtggccta tttctccgat gaccaggcca ctgaatggaa acaaatcgag acggtggaag
     7621 gaatcggcca ccattaccgc ctgctggtgc atgtacgtca aagcagcatc gaaatgaaaa
     7681 aagaggatga ataattatgg caaccacccg caacaaggtg atgtggcagg aagggatgct
     7741 gatgcgtcca caccatttcc agcagcagca gcgttacaac gactacctgg ataaccagcg
     7801 tttccgggcc atgaatgatt tatcctgggg atttaccgaa ctcaccctca acaatgaact
     7861 gctggcgcag ggtaagatca tgattgacag cgcgtcaggc acactgcccg acggcaccgt
     7921 cttttctatc cccgaccagg acgcactgcc cgatccgctg cacccgcaaa attttccgga
     7981 cgaaagaagc cgcaatatct acctcgctct gccggtcgcc agtgatgtga gaaatgaaat
     8041 cagcgacgga cggcgaatcg ggcgttaccg gctgaattat gccgatgtcc gggatttgca
     8101 ttcagaagaa ggtgacgcgc gaacattgac gctgggtcaa ctgacgccgc gcattatgag
     8161 cggtgcagaa gatatgagcg cctatattac gctcccgctt tgccgtatca gcgatcgcca
     8221 tgccgatggc tccctgacgt tggatgacga ttttatcccc tcctgccaga atattcaggt
     8281 cagtaagaaa ctgcgtgttt atctcaaaga ggtacagggg gccattggcg gacgggcaag
     8341 cgatctggca aaccgtattg gctctccggc gcagagcggc atcgcggatg tggcggaatt
     8401 tatgatgttg cagttactta accgtaacca gacccggttt acccatcacg cccgtcgatc
     8461 ccagctccac ccggaagatt tctaccttga tcttgccggg ttgctgggtg aactgatgac
     8521 ctttaccgag ccgtcgcgcc tgccctgccc gcttgatgtg tatgatcatc atgacctgac
     8581 caaaatattt aaaacgctgt taccggaagt caaacgggcg ctgcataccg tactgtcgcc
     8641 aagagccgtc aatctgccgc tgcatctgcg tgacggtatc tggcaggccg atatccatga
     8701 cacagaactg ctgcaatctg ccacctttgt gctggccgtg gcggcaaata tgccggtcga
     8761 tcagatccag cgccagttta tccagcagtc gaaaatttcc tcgccggaaa aaatccgcaa
     8821 tatggtcagt gtgcagatcc cctgtattcc attgcgtgcc cttatggtgg ctccccgcca
     8881 gcttccttac cattccgggt tcagctattt cgaactcgac aagagtggcc aggcctggac
     8941 agaaatggct gccgccgggg ccgttgcact gcatgtttcc ggcagtttcc cggatctgaa
     9001 catgcaactg tgggcgataa gagggtaaga cgatggatga ggggtcactg tctctgccac
     9061 catttaccgg acacgacgaa aaaagccagc gcaactacca tctcgcgctt cgtggcaaca
     9121 gcctgaatcc gatgatcgac gcggccacgc cgctactcgg tatggtaatg cgcctttcaa
     9181 ccatgaacag ccagacgatg ccggagcatc tttttgctca ggtggtgaca gatgttcagg
     9241 ccgtggagca actgcttcag gagcagggct acgaaccggg cgtgattatc tcgtttcgct
     9301 acatcctctg cacgtttatt gatgaagcgg cgctcggtaa cggctggtca aacaagaacg
     9361 agtggatcaa gcagtcattg ctggtccatt tccacaacga agcctggggt ggcgagaaag
     9421 ttttcatcct gctggagcgc ctgatccgtg aacccaaacg ttaccaggat ctgctggaat
     9481 ttctctggat ctgtttttct ctgggatttc gcgggcgcta caaagtggca gcacaggatc
     9541 agggggaatt tgagcagatc taccgccgtc tgtaccacgt tctgcataaa ctgcgcggcg
     9601 atgccccttt cccgctgtta caccaggaca aaaaaaccca gggcgggcgt tatcagctca
     9661 tcagtcgcct gactgttaaa catatttttt gtggtggcgt tgtcgtgctg gcgttgtttt
     9721 acctgttcta tctgttgcgg ctggacagcc agacgcagga catcctgcac cagctaaaca
     9781 aattacttcg ataaggacgt ttatgatcca gattgatctt cccacgctgg taaaacggct
     9841 gaacctgttc tcccgccagg cgctggagat ggccgcctct gaatgtatga gtcagcaggc
     9901 agcggaaatt accgtcagcc atgtgctcat tcagatgctc gccatgccac gcagtgacct
     9961 gcgggttatt acccgccagg gcgatattgg catggaagag ttgcgccagg cgctgacggt
    10021 agagaactac acaaccgccc gttctgcgga cagctacccg gcgttttccc cgatgctggt
    10081 tgagtggctt aaagagggct ggctgctggc gtcggctgag atgcagcaca gcgaactgcg
    10141 cggcggcgtg ttgctgctgg ccctgctgca ttcgccgctg cgttatatac cgcctgctgc
    10201 cgcccggctg ttgaccggca ttaaccgtga ccgtctgcaa caggactttg tgcagtggac
    10261 acaggagtcg gcggaatcag tcgtgccgga tgcagacggt aaaggcgcag gcacaatgac
    10321 ggacgcctct gacaccctgc ttgcccgcta tgccaaaaac atgaccgcag acgcccgtaa
    10381 cggcaggctt gacccggtac tgtgccgcga ccacgaaatc gacctgatga tcgacattct
    10441 ctgccgccgc cgtaaaaaca acccggtggt ggtgggcgaa gcgggcgtgg gcaaaagcgc
    10501 actgattgaa gggctggcgc tgcgcatcgt ggcaggccag gtgccggaca agctgaaaaa
    10561 caccgatatc atgacccttg acttgggcgc attgcaggcc ggggcgtcgg tgaagggtga
    10621 attcgaaaaa cgtttcaaag ggctgatggc ggaggtcatt tcctccccgg tgccggtcat
    10681 tctgtttatc gacgaagcac ataccctgat tggcgcgggc aaccagcagg gcgggctgga
    10741 tatctccaac ctgctcaaac cggcgctggc gcgcggcgag ctgaaaacca tcgccgccac
    10801 cacctggagc gagtacaaaa aatacttcga aaaagatgcc gccctgtcgc gccgcttcca
    10861 gttggtgaag gtcagcgaac ccaacgctgc cgaagccacc attattctgc gcggtctgtc
    10921 ggcggtctat gaacagtctc acggcgtgct gattgatgat gacgccttgc aggccgctgc
    10981 gacattaagc gagcgttatc tctccgggcg tcagttaccg gacaaagcga ttgatgtgct
    11041 ggataccgcc tgcgcccgtg tggccatcaa cctgtcgtcg ccgccgaagc aaatctcggc
    11101 gctgaccact ctgagccacc agcaggaggc ggaaattcgc cagcttgagc gcgagcttcg
    11161 catcggactg cgtaccgaca catcacggat gaccgaggtg ctggtgcagt atgatgaaac
    11221 gctgacggcg ctggatgaac tggaagcggc ctggcaccag cagcagacgc tggtccggga
    11281 gattatcgcg ctgcgccagc agttactggg cgtggcagag gacgatgcgg cgccgttgcc
    11341 ggacgcagat accgtggagg atacgcagcc agagtcagag tcagagtcag agtcagagtc
    11401 agagtcagag tcagagcagg ataataccgg tgccgaaccg gctgatgagg ccgacagaga
    11461 acaaccggaa gagaccgctg aaacagtttc cccggtacag cggctggccc agctcactgc
    11521 cgaactggac gccctgcata acgaccggtt gctggtctcc ccgcacgtcg ataaaaaaca
    11581 gattgcggcg gtgattgccg aatggaccgg cgtgccgctc aaccgcctgt cgcagaatga
    11641 aatgtcggtc atcaccgacc tgacgaaatg gctgggtgac accatcaaag gccaggacct
    11701 ggcgattgcc agcctgcata agcatctact gaccgcacgc gccgacctgc gtcgtccggg
    11761 acgcccgctc ggtgcgttcc tgctggctgg ccccagcggt gtgggtaaaa ccgaaaccgt
    11821 cctgcaactg gcagagctgc tctacggcgg tcgccagtac ctgaccacca tcaatatgtc
    11881 cgaattccag gagaaacaca ccgtctcgcg gctgattggt tcgcctccgg gctacgttgg
    11941 ctacggtgaa ggcggcgtac tgaccgaagc gattcgccag aaaccctact cggtagtact
    12001 gctcgatgaa gtggaaaaag cgcacccgga tgtgctcaac ctgttctacc aggcgttcga
    12061 taagggcgaa atggcagacg gtgaaggccg cctgattgac tgcaaaaata tcgtcttctt
    12121 cctgacgtcc aacctcggct accaggtaat agtcgagcat gccgatgacc cggaaaccat
    12181 gcaggaagta ctgtatccgg tgctggccga cttcttcaaa cctgccctgc tggcgcgtat
    12241 ggaagtggtg ccgtatctgc cgctgtcgaa agagacgctc gccaccatta tcgccggaaa
    12301 actggcccgt ctggataacg tgctgcgcag tcgctttggt gtagaagtgg tcattgaacc
    12361 ggaagtgacg gacgaaatca tgagccgcgt cacccgcgcg gaaaacggcg cgaggatgct
    12421 ggaatcggtc atcgatggcg acatgctacc gccgctctcg ctgctgctgt tgcagaaaat
    12481 ggcggctaac acggcgattg cccggattcg gttgtcggca gtggacggcg catttacggc
    12541 agacgtggaa gatgctcaga acgacgagtc cgtcacaaag gatgaaacgg ttttatgagc
    12601 aggttgcgcg ttttccggca gacaggatgg tttatcgcgg gcctgatgac gggcttaccc
    12661 gccactgccg cgcccgcaga agcatcgtca atggccggtg tggcggtcgc cgtcgccacc
    12721 acgaccccgc cggatgcgac agccacgtta caggccatgc agtcctgtcg gcgggagtcc
    12781 gcggcgctgg agcggcttga ctgctatgac cgcctgctgg caccgctgtc cccgtcaggg
    12841 tttgacgggg cgctggtcaa agccggtttt gtgggcgagg cctggacccg cgccacagag
    12901 caggaaaaac gccgcgaggg caacaccaca gaactgctgg tgacgcaggt gccgggggag
    12961 cgcccgacgg tggtgataac caccccggct atcggtcatg ttccgccacg cccggtgctg
    13021 atgttcagtt gcgtggacaa tatcacccgg atgcaggtgg cgctgatgca ccctctggat
    13081 gtccatgata tcgccgtcac tctgaatgcc gacaaccggg cattgcgcag ccactggttt
    13141 gtgcgcgaga acggcaccct gctggagtcc agccgggggt tatccgggat tgatgaaatt
    13201 aagcaactgt tcggcgcaaa aacgctgacg gtggataccg gcgcagacaa tgccgccgga
    13261 aagctgacct ttaacattga cgggctggca agggcgattg ctcccctgcg cgatgcctgc
    13321 cactgggcgg gagaataaaa atatggcaat ggacttacgc gatccgaatg tctggatatc
    13381 gcacctgctg gaaaacctgc cggaagagaa actggcatcg gcgctgaaag atgacaaccc
    13441 gaactgggag tatatcgacg gcgaaatcgt caagctggga tcgctggccc acagtcaact
    13501 ggatatcccg gaactccagc gccgggggct ggtcattctg gcttcggaga gtaaggattt
    13561 tcgtctgctg gcgcatctgc taagaaccct gcaacacgcc ggtgatcctc atctcgcatt
    13621 gcgcctgctg gcgctgtatg tggagcatta ctgggcggtg gctgcgccgc agaatatggc
    13681 gcataaaaaa cgctttgcca gccaggtcat taaacgcttt gaaacaggta ttgaagtttt
    13741 ttcacagaac gccgccacag cgcagcgcga tgcgctgtca gggcaactgg caaaactggc
    13801 gcaatgctgg cagtcacata acgccccgga actggcacag gctactgatg atctttttgc
    13861 cctgtaccag cgtgcgttta atcgtgcggc accggctcct gtccccactc cggcggcctc
    13921 cggtagttca ccacagacca ccgccacgtc tgaaagcggc gtgacgcaac ccagtgctcc
    13981 ggctccccaa atcgtcatcg acagtcacga cgacaaagcc tggcgcgaca cgctgttaaa
    14041 agtggcggcc attttatgtg aacgccagcc ggattcaccg cagggctacc gtctgcgccg
    14101 ccatgcgctt tggcagaaca tcaccagtac gccccaggcg gaaagcgatg gccgtactcc
    14161 actggctgcg gtctccgccg atatggtggc agattaccat gctcagctcg gtagtgccga
    14221 tatggcgctg tggcagcagg tggagaaaag cgtactgctg gctccttact ggctggatgg
    14281 tcactgcctt tctgcacaga cggcgctgcg tctgggttac aaacaggtgg cagacgccat
    14341 ccgcgatgag gtcatccgct tccttgagcg tctgccccag cttaccgggc tgctgtttaa
    14401 tgaccacaca ccgtttatca gtgagcagac gaaacaatgg ctggcagctt cgcccgacgc
    14461 caaagttgca ccggttgcgc aaatcggtga ggaatcgaag gcagccagag cctgttttgc
    14521 tgagcagggt ctggaggcgg cgctgcgata tctggacatg ctacccgaag gcgatccccg
    14581 cgatcagttt caccgccagt accttgccgc acagttgacg gaagaggcgg ggctggtaca
    14641 gcttgcgcag cagcagtacc ggatgttgtt caggatggga ttgcagatga tggtggcaga
    14701 ctgggagccg tccctgcttg agcagctaga acaaaaattc acggcagaac aataaaagag
    14761 aacaggagtt tttgtgttca gattaccaac accccgacta ctcagcggac tcaaatcagc
    14821 cctgcgaccg gcgatgcccc ggtttaaagt ctctgctttc tggctgctga tactggcgtg
    14881 gatttttctg ctggtgtgga tctggtggaa aggcccgatg tggacgctgt atgaagaaca
    14941 gtggctcaaa ccactggcga accgctggct ggcaacggcg gcgtggggga ttattgccct
    15001 ggtgtggctt accgtccggg tgatgaagcg cctgcaacag cttgaaaaaa tgcaaaagca
    15061 acagcgcgag gaagccgttg atccgctcag cgtggaactg aacgcccagc agcgttatct
    15121 tgaccgctgg ctgctgcgcc tgcaacgcca tctcgacaac cgccgtttcc tgtggcagtt
    15181 gccgtggtat atggtcatcg gcccggcggg cagtggcaaa acgacactgc tgcgcaaggg
    15241 gtttccgtcc gacattattt atgccccgga gggcgcacgc ggcgcagaac aacgcctgta
    15301 cctcacgccc catgtcggta aacaggcggt gatctttgat atcgacggca cactctgcgc
    15361 tcccgctgat gcggatatcc tgcatcgccg tctgtgggaa catgccctcg gctggctgaa
    15421 agaaaagcgc gcgcgccagc cgctgaacgg gattattctg acactcgatt tacccgatct
    15481 gctgaccgca gacaaacgcc gccgcgagca tctgttacag acgctgcgca gccgcttaca
    15541 ggatatacgc cagcatcttc actgccagtt accggtttac gtggtactga cccggcttga
    15601 tttattacag ggttttgccg ccctgttcca gtcactcaac agacaggatc gcgatgcgat
    15661 tctgggcgtc acgttcaccc gccgtgccca tgaaaatgat gactggcgaa cagagttgaa
    15721 tgctttctgg cagacatggg tggatcgaat gaatctggcg ttgccggatc tgatggtcgc
    15781 tcagactcac acccgcgcgt ctttattcag tttttcccgc cagatgcagg gaagccgtga
    15841 accgctggtg tcactgcttg agggtctgct tgatggcgaa aatatgaacg tgatgctgcg
    15901 tggtgtctat ctcacctctt cgcttcagcg tggacagatg gatgatatat tcacccagtc
    15961 tgccgcccgc cagtaccggc tgggcaataa cccactggcg tcctggcccc tggtggacac
    16021 cgcgccttat ttcacccgca gcctgttccc gcaggcatta ctcgcagagc ctaatctggc
    16081 aacagagagc cgcgcctggc tgatacgttc ccgtcgccgc ctgacggttt tctctgccac
    16141 aggcggcgtg gcagcactgc tgctcatcac cggctggcat cactattaca acggtaacta
    16201 tcagtccggc atcaccgtgc ttaagcaggc caaagccttt atggacgtgc cgcctccgca
    16261 gggggaagat gactttggca acctgcaact gccgctcctg aacccggtac gcgatgccac
    16321 actggcctat ggcgactggg gcgaccgcag ccgtctggcc gatatgggac tgtaccaggg
    16381 acgacgtatc gggccttatg tggaacagac ctatctgcaa ctgctggagc aacgttacct
    16441 gccctcgctg tttaacgggc tggtcaaagc gatgaacgcc gcgccgccgg agagtgaaga
    16501 aaaactcgcg gtgctgcgcg tgatgcgaat gctggaggac aaaagcggac gtaacaatca
    16561 ggtggtgaag cagtatatgg caaaacgctg gagcgaaaag ttccacggtc agcgcgatat
    16621 ccaggcacaa ctgatgtccc atcttgacta cgcgctggct catactgact ggcacgcaga
    16681 gcgtcaggcg ggcgacggtg acgccatcag ccgctggacg ccatatgaca agcccgtggt
    16741 atcagcacag aaagaactga gcaaactgcc tgtctaccag cgggtttacc agagcctgaa
    16801 aacgcgggcg ctgggcgttc ttcctgccga cctcaatctg cgtgaccagg tagggccaac
    16861 ctttgaccag gtgtttacat ctgccgatga caacaaactg gttgttccac agtttatcac
    16921 ccgctacggc ctgcaaagct attttgtaaa acagcgcgat gaactggttg aactgacagc
    16981 gatggattcc tgggtactta acctcacccg caatgtgaaa tacagtgacg ccgaccgtgc
    17041 ggaaatccag caccagttga ccgagcagta tatcagcgac tacaccgcca cctggcgggc
    17101 tgggatggac aatctgaata tccgcaattt tgagtccatc ggacaactga ccggggcgct
    17161 ggagcaggtt atcagcggcg accagccttt gcagcgggcg ctgaccgtgc tgcgtgacag
    17221 cacacagcca ggcgtctttt ctgaaaaact ctctgccaaa gaacgggagg aagccctggc
    17281 agagccggat taccagttac tcacccgcct cgggcatgaa ttcgccccgg aaaacagtac
    17341 cctggcagta cagaaagaca aagaaagcac gatgcaggcc gtgtatcagc aactcaccga
    17401 gttgcaccgc tacctgctgg caatccagaa cgcgcctgta ccagggaaat cggcgctgaa
    17461 agccgtgcag ttacggcttg atcagaacag cagcgatccg atattcgcca cccgccagat
    17521 ggcaaaaacg ctgcctgctc cgctcaaccg ctgggttggc agactgactg actaggcctg
    17581 gcatgtggtg atggtggagg ctgttcatta tatggaagtg gactggcgcg acagcgtggt
    17641 gaaaccgttt aacgagcaac tggcaaataa ctatccgttt aatccgcgtt ctgcacagga
    17701 tgcctcactg gatgccttcg aacgcttctt taaaccggat ggcatactgg ataccttcta
    17761 ccagcagaac ctgaagctgt ttatcgataa tgacctgagt ctggaggatg gcgataacaa
    17821 cgtcattatt cgcgaagata ttattgcgca actggaaact gcgcagaaaa tccgtgacat
    17881 cttcttcagc aaacagaacg gtctgggaac atcctttgcc gtggaaacgg tatcgctttc
    17941 aggcaataaa cgccgcagtg tactgaacct tgacagtcag ttagtcgatt acagccaggg
    18001 ccgtaactat accgcccatc tggtctggcc taacaacatg cgcgaaggca acgaaagtaa
    18061 gctgacgctc atcggcacca gcggcaacgc gccgcgcagt atcagcttca gcgggccgtg
    18121 ggcgcagttc cgcctgttct gggccggaca actgaccgga gtacaggatg gcaactttac
    18181 cgtgcgcttt agcgtggacg gtggcgcgat gacctaccgt gtgcataccg acacggaaga
    18241 taacccgttc agcggtgggt tgttcagcca gtttggtctg tcagacacac tgtactgagg
    18301 aggcagggat gaatagtaac gtactgacac aaactatcgt taccggcagt gacccgcgcg
    18361 ggctgccgga attcagcgcc atccgcgagg aaataaacaa agccagccac ccgtcacagc
    18421 ctgagctgaa ctggaaactg gtggagtcgc tggcgctggc gatttttaaa gccaacggtg
    18481 tggatttaca caccgccacc tactatacgc ttgcccggac acggacacag ggactggcgg
    18541 gattctgcga aggtgcggaa ctgctggcgg caatggtaag ccacgactgg gataagttct
    18601 ggccgcaggg cggcccggcg cgtactgaaa tgctggactg gtttaactcc cgaaccggca
    18661 atattctgcg tcagcaaatc tcctttgcgg aatccgacct accactgatc taccgcacag
    18721 agcgggcatt gcagcttatc tgcgataagc tccagcaggt ggaactgaag cgcgttccgc
    18781 gcgtggagaa tctgctctat tttatgcagc acacgcgtaa acggcttgaa ccccagccga
    18841 agagtaacgc tgagaacgcc gcacagacca cggtcagaac gctgatttat gccccggaaa
    18901 cactggcatc ttccacacca gaagcggtag tgcctcccct gcccggcctg cctgagatga
    18961 aagtggaagt gcgcagtctg acagagaatc ccccacaggc cagtgtgata aagcaaggca
    19021 gtacggtaag agggtttatc gcagggatcg cctgttcagt ggctgtcgcc tcagcattgt
    19081 ggtggtggca ggtctatccg gtgcagcagc aactgttaca ggttaacgac accgctcagg
    19141 gcgcagcaac ggtgtggatg gcctcacctg aactcgaaaa ctatgagcgc aggctgcaac
    19201 aacttcttga tacctccccg gtacagccgc tggaaaccgg gatgcagatg atgcgtgttg
    19261 ccgacagtcg ctggccggaa agcctgcaac agcaacaggc ctcgacacaa tggaatgagg
    19321 cactcaaaac ccgcgcacag agtagcccgc agttgcgtgg ctggttgcag acccgccagg
    19381 acttacatgc ttttgcagat ctagtgatgc agcgcgagaa agagggacta accctttcct
    19441 atatcaaaaa tgtcatctgg caggcggagc ggggactggg gcaggaaacc cccgttgagt
    19501 ctctgttgac gcagtaccat gatgcccgtg cgcagaagca gaatacagat gcgctggaaa
    19561 aacaaattaa tgagcgactc gaaggcgtgt taagccgctg gctgctgctg aagaataacg
    19621 tcatgccaga ggcggcaacc ggtaccacgg ccgaaaaata attatttaat taaaggagat
    19681 tatcatggca aatataagtt atttatcttt gagtggagag acacaagggc taatttcagc
    19741 aggttgctca acgcttgatt ctgtcggaaa caaagcgcag ccagaacata aagatcagat
    19801 catggtttat gcgttaatgc attccatctc aagaagtcaa aatgtcaatc atcatgaatt
    19861 aattatcacc aaaccggttg ataaatcctc accattgctg gcaaaagcac ttagtgacaa
    19921 tgaaaaaatg gcaatatgtg aatttatact ctaccgaacc agtaaagcgg gtatttacca
    19981 gccttattat aaaataaatc tgtcaaaagc ccgaatatca agtattgatt ttgtcacacc
    20041 gcatgctgtc ctggaaaaag aactggaacc acaggagaga attgccttta tttatgaaga
    20101 tatttcctgg gagcatacgt tagccggtac gaatgcaatg agtaaatggc aggatcgtgt
    20161 acagtaactt actgtaataa cataataaat tactattgcg gagagataaa atggctggtt
    20221 taaggaataa caacaacaca cagaatgcac agtgggctga ttatgtgggt gacattcttc
    20281 ggggggctca gccgataaac cagctagtac ctcagcaccc ttatcttaac gacgtcccac
    20341 tcattgatga gttaaggcat cagaacactc atcatgttat tctcttgaca cttgatgttg
    20401 caaaaaaaaa aaaaaaaaaa aatactcagc ccaataacat catttgatta cattcatttt
    20461 atcactacac atccttcagg tataaaagat acactcgcct ggttagttaa cgcgagtaaa
    20521 ttaatgactg agtttgatga caatgggaaa attatattta acattaatgc cttaaagtat
    20581 acaaaagcaa gttactttga aatacttgga gagaaatata taaaaatcac aacatccagc
    20641 ccctggcttt tagaaaagct cggtaaatat attttctctt cacgagcgcc tcaagttctg
    20701 gaactcgcta ttggctggcg aggagcttta tctgaatcaa taaaaggagt taagttctgt
    20761 atctggtttt cagtagcctg gagaaccata gaatttatta tgagttcaga gcgagatctg
    20821 gttaattttc ttggcgattt ttcaatggat gtggccaaag cagttatagc cggtggtgtt
    20881 gcaaccgcta ttggaagcct ggcttctttt gcctgtgtta gctttggctt tccagtaatt
    20941 cttgtcggag gagcaatttt actgacaggg atagtgtgta cagttgtttt aaatgaaatc
    21001 gatgctcaat gccatttatc agaaaaatta aaatatgcaa ttagagatgg actaaaacgg
    21061 caacaggaac ttgataaatg gaaaagggaa aacatgactc catttatgta tgttcttaac
    21121 actccacccg tgatatgagg attaaccatg cgtttacatg ttaaattaaa agagtttctt
    21181 tcgatgtttt ttatggccat attatttttc cctgcattca atgcttcttt gttttttacc
    21241 ggagttaaac ctctatattc aataataaaa tgctccacgg aaatatttta tgattggaga
    21301 atgttgattc tgtg
//
LOCUS       DAZPLI010000064        21005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZPLI010000064 DAZPLI010000000
VERSION     DAZPLI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.065"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..172)
                     /locus_tag="ACHUU3_002255"
     CDS             complement(<1..172)
                     /locus_tag="ACHUU3_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HFR2082303.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            344..1564
                     /gene="coaBC"
                     /locus_tag="ACHUU3_002256"
     CDS             344..1564
                     /gene="coaBC"
                     /locus_tag="ACHUU3_002256"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR2082304.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1542..2000
                     /gene="dut"
                     /locus_tag="ACHUU3_002257"
     CDS             1542..2000
                     /gene="dut"
                     /locus_tag="ACHUU3_002257"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR2082305.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2107..2703
                     /gene="slmA"
                     /locus_tag="ACHUU3_002258"
     CDS             2107..2703
                     /gene="slmA"
                     /locus_tag="ACHUU3_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR2082306.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2740..3381)
                     /gene="pyrE"
                     /locus_tag="ACHUU3_002259"
     CDS             complement(2740..3381)
                     /gene="pyrE"
                     /locus_tag="ACHUU3_002259"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR2082307.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3447..4163)
                     /gene="rph"
                     /locus_tag="ACHUU3_002260"
     CDS             complement(3447..4163)
                     /gene="rph"
                     /locus_tag="ACHUU3_002260"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR2082308.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4290..5153
                     /locus_tag="ACHUU3_002261"
     CDS             4290..5153
                     /locus_tag="ACHUU3_002261"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR2082309.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDVIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5375..6199
                     /gene="dinD"
                     /locus_tag="ACHUU3_002262"
     CDS             5375..6199
                     /gene="dinD"
                     /locus_tag="ACHUU3_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR2082310.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6491..7108
                     /locus_tag="ACHUU3_002263"
     CDS             6491..7108
                     /locus_tag="ACHUU3_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR2082311.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7105..8787)
                     /gene="ligB"
                     /locus_tag="ACHUU3_002264"
     CDS             complement(7105..8787)
                     /gene="ligB"
                     /locus_tag="ACHUU3_002264"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR2082312.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9045..9668
                     /gene="gmk"
                     /locus_tag="ACHUU3_002265"
     CDS             9045..9668
                     /gene="gmk"
                     /locus_tag="ACHUU3_002265"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR2082313.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9723..9998
                     /gene="rpoZ"
                     /locus_tag="ACHUU3_002266"
     CDS             9723..9998
                     /gene="rpoZ"
                     /locus_tag="ACHUU3_002266"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR2082314.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10017..12125
                     /gene="spoT"
                     /locus_tag="ACHUU3_002267"
     CDS             10017..12125
                     /gene="spoT"
                     /locus_tag="ACHUU3_002267"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR2082315.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12132..12821
                     /gene="trmH"
                     /locus_tag="ACHUU3_002268"
     CDS             12132..12821
                     /gene="trmH"
                     /locus_tag="ACHUU3_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR2082316.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12827..14908
                     /gene="recG"
                     /locus_tag="ACHUU3_002269"
     CDS             12827..14908
                     /gene="recG"
                     /locus_tag="ACHUU3_002269"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR2082317.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15077..16282)
                     /gene="gltS"
                     /locus_tag="ACHUU3_002270"
     CDS             complement(15077..16282)
                     /gene="gltS"
                     /locus_tag="ACHUU3_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR2082318.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16562..17953
                     /gene="xanP"
                     /locus_tag="ACHUU3_002271"
     CDS             16562..17953
                     /gene="xanP"
                     /locus_tag="ACHUU3_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR2082319.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18074..19783
                     /locus_tag="ACHUU3_002272"
     CDS             18074..19783
                     /locus_tag="ACHUU3_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR2082320.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKHQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19835..>21005)
                     /gene="yicI"
                     /locus_tag="ACHUU3_002273"
     CDS             complement(19835..>21005)
                     /gene="yicI"
                     /locus_tag="ACHUU3_002273"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR2082321.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5084 a         5716 c         5386 g         4819 t
ORIGIN      
        1 agccataaag agagccgaaa ttctccagca tctcttttgc cagggttaat acatctttac
       61 cgcgcgttcc ggtacgcaga aatagcgcca gcagctcgac atccgttaag gcgctaatac
      121 caaacttcag cattttttcg cgcggcatca acagctgtgc attgtttttc accttcacct
      181 cctttgtggt gctcgcatcc tgtcatagcc gtttcgttaa atcgacggcc agttttcaat
      241 cctggaaagc gcctcgcaaa gtgaatctca ggtgaaggca cgacaacaaa agtattgtga
      301 taaaatcgcc aacttctggt gtcaccctac aggaaaaatc atcatgagcc tggccggtaa
      361 aaaaatcgtt ctcggcgtta gcggcggtat tgctgcctat aaaacccctg aactggtgcg
      421 tcgtttgcgc gatcgcgggg ccgacgtccg cgtagccatg accgaagcgg caaaagcctt
      481 tatcacccca cttagcttgc aggcggtttc tggttatccc gtttccgaca gtctgctgga
      541 cccggcagcc gaagccgcta tgggccatat tgagctgggt aaatgggctg atttagtgat
      601 tctcgcccct gccacggcag atttgattgc ccgtgttgct gccggaatgg cgaatgacct
      661 ggtatcgacg atttgtctgg ctacacctgc gcctgtagcc gtgctccccg ccatgaacca
      721 gcagatgtac cgtgccgctg ccacgcagca taatttagag gtgcttgctt cccgtggttt
      781 gctcatctgg gggccagaca gtggcagtca ggcttgtggt gatatcggtc ctgggcgaat
      841 gctcgatccg ttaaccattg tggatatggc ggtagcgcat ttttcgcccg tcaacgacct
      901 gaaacatctg aacattatga ttaccgccgg cccgacgcgt gaaccgctcg atccggtgcg
      961 ttatatctct aatcacagct ccggcaagat gggttttgct atcgccgccg ccgctgcccg
     1021 tcgtggcgcg aacgtcacgc tggtatcagg cccggtttcg ctaccgacgc caccgtttgt
     1081 taaacgtgtt gatgtgatga ccgcgctgga aatggaagtc gccgtgaatg cttctgtaca
     1141 gcagcaaaat atttttatcg gctgcgccgc cgtggcggat tatcgcgcag ctaccgtggc
     1201 cccagagaaa atcaaaaagc aggccacgca gggtgatgaa ttaacaataa aaatggttaa
     1261 aaaccccgat atcgtcgcag gcgttgccgc actaaaagac catcgaccct acgtcgttgg
     1321 atttgccgcc gaaacaaata atgtggaaga atacgcccgg caaaaacgta tccgtaaaaa
     1381 ccttgatctg atctgcgcga acgatgtttc ccagccaact caaggattta acagcgacaa
     1441 caacgcatta caccttttct ggcaggacgg agataaagtc ttaccgcttg agcgcaaaga
     1501 gctccttggc caattattac tcgacgagat cgtgacccgt tatgatgaaa aaaatcgacg
     1561 ttaagattct ggacccgcgc gttgggaagg aatttccgct cccgacttat gccacctctg
     1621 gctctgccgg acttgacctg cgtgcctgtc tcgacgacgc cgtagaactg gctccgggtg
     1681 acactacgct ggttccgacc gggctggcga ttcatattgc cgatccttca ctggcggcaa
     1741 tgatgctgcc gcgctccgga ttgggacata agcacggtat cgtgcttggt aacctggtag
     1801 gattgatcga ttctgactat cagggccagt tgatgatttc cgtgtggaac cgtggtcagg
     1861 acagcttcac cattcaacct ggcgaacgca tcgcccagat gatttttgtt ccggtagtac
     1921 aggctgaatt taatctggtg gaagatttcg acgccaccga ccgcggtgaa ggcggctttg
     1981 gtcactctgg tcgtcagtaa cacatacgca tccgaataac gtcataacat agccgcaaac
     2041 atttcgtttg cggtcatagc gtgggtgccg cctggcaagt gcttattttc aggggtattt
     2101 tgtaacatgg cagaaaaaca aactgcgaaa aggaaccgtc gcgaggaaat acttcagtct
     2161 ctggcgctga tgctggaatc cagcgatgga agccaacgta tcacgacggc aaaactggcc
     2221 gcctctgtcg gcgtttccga agcggcactg tatcgccact tccccagtaa gacccgcatg
     2281 ttcgatagtc tgattgagtt tatcgaagat agcctgatta ctcgcatcaa cctgattctg
     2341 aaagatgaga aagacaccac agcgcgcctg cgtctgattg tgttgctgct tctcggtttt
     2401 ggtgagcgta atcctggcct gacccgcatc ctcactggtc atgcgctaat gtttgaacag
     2461 gatcgcctgc aagggcgcat caaccagctg ttcgagcgta ttgaagcgca gctgcgccag
     2521 gtattgcgtg aaaagagaat gcgtgagggt gaaggttaca ccaccgatga aaccctgctg
     2581 gcaagccaga tcctggcctt ctgtgaaggt atgctgtcac gttttgtccg cagcgaattt
     2641 aaataccgcc cgacggatga ttttgacgcc cgctggccgc taattgccgc gcagttgcag
     2701 taatatgacg ccggatgact tttcatccgg cgagtttctt taaacgccaa actcttcgcg
     2761 ataggcctta accgccgcca gatgttccgc catttccggc ttctcttcca ggtaagcaat
     2821 caggtctttc agggtgatga tagagatcac tttgcagttg taatcacgct caacttcctg
     2881 aatcgccgaa atctcgctgc gcccccgttc ctgacgatcg agcgaaatca acacgccagc
     2941 aagcgtcgcg ccattggcct gaataatttc catcgactca cgaatcgccg ttccggcggt
     3001 gatcacatca tctaccagca ttacgcgtcc ttgtaacgcg ctaccaacca gattgccgcc
     3061 ttcaccgtgg ttttttgcct ctttgcggtt aaagcagtac ggcaggtcca ggtcatgatg
     3121 ctccgccagc gccacggcgg ttgtggtggc aatcgggatc cctttgtaag cagggccaaa
     3181 cagcagatcg aactcaatgc cggaatccac caatgcttca gcgtaaaaac ggcctaacag
     3241 tgccaggtcg cgcccggtat taaacagccc ggcgttgaag aaataggggc ttttacgccc
     3301 ggatttcagc gtaaactcgc caaactttaa cacctgcttg ctaagcgcaa attcaataaa
     3361 ctggcgctga tatggtttca tgccttcgct cctcatctta cttttctaca gacaaaaaaa
     3421 aggcgactca tcagtcgcct taaaaatcag tttgccagcg ccgccttctg cgtcgctaca
     3481 atggattcga ttccccctcg ggccagagcc aacaaggtga gtaattcttc gtgggtgaac
     3541 ggttcgcctt ctgccgtccc ctgcacttca atgatgcgcc cgtcttcggt catcactacg
     3601 ttcatgtcgg tctctgcggc agagtcttca acgtattcca gatcgcaaac cgcttcgccg
     3661 ttcacaatcc cgacagaaac cgcggctacc atccctttca tcggattggt tttcagctta
     3721 ccgttttcca ccagcttctg tagcgcatct gccagcgcca cgcaggcacc cgtaatcgaa
     3781 gcggtacgag tgccaccatc agcctgaagc acgtcgcagt ccagcgtaat ggtgaactca
     3841 cccagcgctt tcaaatctac tgccgcgcga agcgcacggg cgatcagacg ctggatttcc
     3901 attgtgcgtc caccctgctt acctttcgcc gcttcacgag cgttacgggt gtgggtagaa
     3961 cgcggcagca taccgtactc tgcggtgatc cagccctggc cctgaccttt caggaagcgc
     4021 ggcacgcctt cttcaataga ggcggtacac aacactttgg tatcgccaaa ttcgaccagc
     4081 accgagcctt ctgcatgttt tgtatagtta cgagtcaggg taacgggacg cacctgatta
     4141 ttgctacggc ctgctggacg catattgaaa tctccggctt gaaacaaatg tggctgcgca
     4201 ttatacggac ttccggaggt tattcctatc ctgacaaggc atcgatggct ataatccttc
     4261 cacctctcct tttataaaca ggaacgtcta tgatccgcag tatgaccgcc tacgcccggc
     4321 gtgaaatcaa gggtgaatgg gggagcgcaa cctgggaaat gcgctcggta aaccagcgtt
     4381 atctggaaac ttactttcgt ctgccggagc agttccgtag ccttgaacct gtcgttcgcg
     4441 agcgtattcg ttctcgcctg acgcgcggta aagtggaatg taccctgcgc tatgagccag
     4501 atgttagcgc gcaaggtgag ctgatcctca acgaaaaact ggctaaacag ctggtaactg
     4561 ccgcgaactg ggtaaaaatg cagagtgacg aaggggaaat caacccggtt gatattctgc
     4621 gctggccggg cgtaatggca gcccaggagc aggatctcga cgtgattgcc gctgaaattc
     4681 tcgcggcgtt ggatggtacc ctggacgact ttatcgtcgc acgtgaaacc gaaggtcagg
     4741 cgctgaaagc gttgatcgag cagcgtctgg aaggcgtcac cgccgaagtg gtcaaagtcc
     4801 gcgcccatat gccggaaatc ctgcaatggc agcgtgagcg tctggtcgcg aagctggaag
     4861 atgctcaagt gcaactggaa aacaaccgcc tggagcagga gttggttctg ctggcacaac
     4921 gaattgatgt tgccgaagaa ctggaccgcc tggaagcgca tgtcaaagag acctacaaca
     4981 ttctgaagaa aaaagaagcc gttggtcgtc gtctggactt tatgatgcaa gagttcaacc
     5041 gcgagtcgaa cactcttgcg tcgaaatcta tcaatgccga agtgacgaat tctgccatcg
     5101 agctgaaagt gttgattgag cagatgcgcg agcagattca gaatattgag taactcgtaa
     5161 cactaattct tacctttcag ccttcacaaa tgccgcccac tcaaacagag cggcattttt
     5221 cttccccgca acattcaatt ctgttttgcg tacctgctcc agattttgcg atgttttttt
     5281 gcgcagcgca gtgagaacgt gagatactca caactgtata taaatacagt tatagactta
     5341 ctttctttgc aatcgatatc acatggagtg ggcaatgaac gaacatcatc aaccttttga
     5401 agaaatacgg cattatggca ctgaagggca agaattttgg tcagcgagag aattggcacc
     5461 gttactggat tatcgggact ggcgcaattt tcaaaaggta ctggcccgcg cgactcaagc
     5521 gtgcgaagcc agcaatcaag ctgcatctga ccatttcgtt gaaaccacca aaatggttgt
     5581 attaggttca ggggctcaac gagaacttga agatgttcat ctctctcgct atgcctgtta
     5641 cctggtagta caaaacggcg accctgcgaa accggttatt gcggcagggc aaacttattt
     5701 tgctatccag acccgacggc aggagctggc tgacgatgaa gctttcaggc aacttcgtga
     5761 agacgaaaag cgtctttttc tgagaaatga gttgaaagaa cataacaaac aattagttga
     5821 ggcagcgcag caagctgggg tagcgacagc tatcgatttc gccatatttc agaatcatgg
     5881 ttaccggggg ctgtatggtg gattagatca gaaagccatc catcagcgga aggggctgaa
     5941 aaagagtcag aagatcctgg atcatatggg ttcaacagaa ctggcggcta atctctttcg
     6001 agctacccaa acagaagaaa aactcaagcg ggatggcgta aattcaaaac agcaagctaa
     6061 taccacacac tttgacgtgg gtagcaaggt gaggcaaacc attcaggaac ttggcggaac
     6121 catgcctgag gagttgccga ccccgcaggt cagcatcaat cagttggaaa acagtgtaaa
     6181 aattacagag aagaaatagc agaaactaaa aactcttttt cttaattgag atacccgatg
     6241 cgtaagccaa agtccaggtg cagtgaacac aatggctaaa cattgcacct ttcttttcca
     6301 cccctcaatt ttaacctatt ttttcttatg cattttctca gacaagaagt cagaagaatg
     6361 catctacact acagaaaata gcgatttcac ataactacaa gttatcaatt tcctctcacc
     6421 ttaaaaaatc tcaatcgtga caatacgcac aaatcgctac cctgccagac agatttttag
     6481 ggagagaacc atgctgttac acattttgta tttggttggc attactgccg aagccatgac
     6541 gggggcgctg gcggccggac gacggcgcat ggatacattt ggcgtaatta ttattgcgac
     6601 cgccaccgca attggcggag ggtcagtgcg cgatattctg ctgggccact atccgctcgg
     6661 ctgggtcaaa cacccggaat atgtgattat cgtcgctacc gccgcagtac tcaccactat
     6721 cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg gtgctcgatg cgctcggact
     6781 ggtggtcttt tctatcatcg gcgcacaggt tgcactggat atggggcacg gcccaattat
     6841 tgccgttgtc gcggcggtaa ccaccggcgt gtttggcggc gttttgcgcg atatgttctg
     6901 taaacgcatc ccactggtat tccagaaaga gttgtatgcc ggtgtctcat ttgcctccgc
     6961 cgtgctgtac atcgcgctgc aacactatgt ttctaaccat gatgtggtga ttatctccac
     7021 cctggtattc ggcttttttg cccgcttact ggcgctacgt cttaagctgg gattaccggt
     7081 cttttactac agccacgaag gtcactaagg ttcaaaacct gtgatctgct gggcagccag
     7141 ccaactgccc agcttcttga tttgcgcatt ttccttccat tcaataacct gtctggcgcg
     7201 cccagatcca gtccccggca gccgctgcca gaactgctcc gtgctaaata aaagttgcga
     7261 ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt aacggtattc ccattgccat
     7321 cacccagcga gtaaaaggct gcttacgagc cagattaaac tgatgccata actgcgcact
     7381 tttacttttc gcgatccccg gcgtgttctg taattgctct ggcgttaata aaagccagga
     7441 aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc cagccggcct caccaatgcc
     7501 atccagccca agaacctgtt ttgaccccag ccagactaag cgtgaaatga actgttcctg
     7561 acaaacatca gaagcaaagt agcaggtcaa cgagttaaag cggttttctg gtggtgtcgg
     7621 ttttgtacgt tctgcaccgc gccagaccac atcatcaatg cgaggaatac cctgaccggc
     7681 aaggctgacg aggatctgat caccaggcgc aatatcccac tcctgccagc gcctgacgga
     7741 accaatattc acccgctgga cttttttatc atccagcatg acaggtgcga gtgacgcaac
     7801 caccgatatt ttaccgctct tacccaccgc aaactgaatc gccttcactt cggcaacctg
     7861 agctacaggt tgatatttcc aggccaccaa ccattctgcc tggcctggta gccaatggcg
     7921 ggattcgggt tccttagccg ctcgcacaac cacgccatcg gtgacgaagg gtaatttcgc
     7981 tttccaccac tcattgcgta cgcgcgcaac ttcatcagca tttttaaccg cacgggtata
     8041 cctctgcgtt agagtaaaac ctgcggtagc cagctccttt aaacgatcgg tcattaactg
     8101 cggtccatcc ggccatgccc agacaaaaac agccagtgag tttagcgtgt cgctattccc
     8161 ctggcgcatc atcaggccag caacttttgc gcgggcattt attcccccca tttgttgttg
     8221 gatatgcccc tcgcgctgga gaaatatttc cccctgaagc gtactgttgg ctaaaggccc
     8281 gctaacggtt tgcggcacag cggaaattaa gctaactttc tgcgtccagt cctcgccttt
     8341 caggccatta ccgcgactga ttgctttgtt cagtttcccg tcccgataaa ccagggttac
     8401 cgcaacgcca tcaacttttg gctgcaccca aagatcgcta cgttctcgca tccacagact
     8461 taatgcgttt ttatccacca ttttgcgtac acccgtatgg gcaaccggat gcataaccgc
     8521 accatttaaa ggcggcatca tcacgtcgcg gggctcgctc ccaaagcagc gttgccactg
     8581 cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc acctcacttt tcccttcctt
     8641 ccagtagtca tcgtcccact gttttatttg ctgttgcagg cgggaaattt cttcctgtgc
     8701 tctggctggc gaccaggccg gacagaccgc ccacacagat gattgccagc acaagatact
     8761 tattaatatc gccatccata ctttcatcat cacctccgct gtagatagtc aggcagatat
     8821 acaacgtgat gaaagcaaag ccgagtggca aaaacggagt ctgcgaggac gcttcctgaa
     8881 aatcgtcttt attgcaatga atgacaggca aatgcggaag cagctacgca aaacgcaaca
     8941 actttgcgca aaaagtgtga gcaagggcta cgtcacatgg ccgcgccgtg tataataagc
     9001 tcgtatgtag gctttatttc gctaatcaca tacgaaagat actcatggct caaggcacgc
     9061 tttatattgt ttctgccccc agtggtgcgg gtaaatccag cctgattcag gctttattaa
     9121 aaacccaacc gttgtatgac acccaggttt ctgtttcaca caccacacgc caaccgcgtc
     9181 ctggtgaagt ccacggtgaa cattatttct ttgttaatca tgatgaattt aaagaaatga
     9241 ttagcagaga tgcgttcctc gaacacgcag aagtttttgg taattactat ggcacttcgc
     9301 gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt ttttctcgat atcgactggc
     9361 agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg gagtatcttt attttaccgc
     9421 cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg tcaggacagc gaagaggtca
     9481 ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca ttacgccgaa tatgattatc
     9541 tgattgtgaa tgatgacttc gataccgcgt tgaccgattt gaagaccatt attcgcgccg
     9601 aacgtctgcg catgagccgc caaaagcagc gtcatgacgc tttaatcagc aaattgttgg
     9661 cagactgaac ctgatttcag tatcatgccc agtcatttct tcacctgtgg agctttttaa
     9721 gtatggcacg cgtaactgtt caggacgctg tagagaaaat tggtaaccgt tttgacctgg
     9781 tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg cggaaaggat ccgctggtac
     9841 cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga aatcgaagaa ggtctgatca
     9901 acaaccagat cctcgacgtt cgcgaacgcc aggaacagca agagcaggaa gccgctgaat
     9961 tacaagccgt taccgctatt gctgaaggtc gtcgttaatc acaaagcggg tcgcccttgt
    10021 atctgtttga aagcctgaat caactgattc aaacctacct gccggaagac caaatcaagc
    10081 gtctgcggca ggcgtatctc gttgcacgtg atgctcacga ggggcaaaca cgttcaagcg
    10141 gtgaacccta catcacgcac ccggtagcgg ttgcctgcat tctggccgag atgaaactcg
    10201 actatgaaac gctgatggcg gcgctgctgc atgacgtgat tgaagataca cccgccacct
    10261 accaggatat ggaacagctt tttggtaaaa gcgtcgccga gctggtagag ggggtatcga
    10321 aacttgataa actcaagttc cgcgataaga aagaggcgca ggccgaaaac tttcgcaaga
    10381 tgattatggc gatggtgcag gatatccgcg tcatcctcat caaacttgcc gaccgtaccc
    10441 acaacatgcg cacgctgggc tcacttcgcc cggacaaacg tcgccgcatc gcccgtgaaa
    10501 ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat ccaccacatt aaaaccgaac
    10561 tcgaagagct gggttttgag gcgctgtatc ccaaccgtta tcgcgtaatc aaagaagtgg
    10621 tgaaagccgc gcgcggcaac cgtaaagaga tgatccagaa gattctttct gaaatcgaag
    10681 ggcgtttgca ggaagcggga ataccgtgcc gcgtcagtgg tcgcgaaaag catctttatt
    10741 cgatttactg caaaatggtg ctcaaagagc agcgttttca ctcaatcatg gacatctacg
    10801 ctttccgcgt gatcgtcaat gattccgaca cctgttatcg cgtgctcggg caaatgcata
    10861 gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat cgccattcca aaagcgaacg
    10921 gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg tgtgccggtt gaggtccaga
    10981 tccgtactga agatatggat cagatggcgg agatgggtgt tgccgcgcac tgggcttata
    11041 aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc ccagcgctgg atgcaaagcc
    11101 tgctggagct gcaacagagc gccggtagtt cgtttgaatt tatcgagagc gttaaatccg
    11161 atctcttccc ggatgagatt tacgttttca caccggaagg gcgaattgtc gagctgcctg
    11221 ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac cgatatcggt catgcctgcg
    11281 tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca gccgcttacc agcggtcaaa
    11341 ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc cgcgtggctg aactttgtcg
    11401 ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa aaacctcaag cgtgatgatt
    11461 ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg tggtagccgt aagctgaatg
    11521 aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat gaagctggca acgcttgacg
    11581 atctgctggc agaaatcgga cttggtaacg caatgagcgt ggtggtcgcg aaaaatctgc
    11641 aacatgggga cgcctccatt ccaccggcaa cccaaagcca cggacatctg cccattaaag
    11701 gtgccgatgg cgtgctgatc acctttgcga aatgctgccg ccctattcct ggcgacccga
    11761 ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca ccatgaatcc tgccgtaata
    11821 tccgtggcta ccagaaagag ccagagaagt ttatggctgt ggaatgggat aaagagacgg
    11881 cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa tcatcagggc gcgctggcaa
    11941 acctgacggc ggcaattaac accacgactt cgaatattca aagtttgaat acggaagaga
    12001 aagatggtcg cgtctacagc gcctttattc gtctgaccgc ccgtgaccgt gtgcatctgg
    12061 cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat taaagtcacc cgaaaccgaa
    12121 attaatgttt tatgaaccca acacgttatg cacgcatctg cgaaatgctc gccaggcggc
    12181 agcctgatct gaccgtctgc atggagcagg tccacaaacc tcataacgtt tctgcgatta
    12241 ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc cgtctggcct ggtagccgca
    12301 tgcgcaccat ggcttcggca gcggcgggta gtaacagctg ggtacaggtg aaaacacacc
    12361 gcaccattgg cgatgccgtc gctcatctga aaggccaggg catgcagatt ctggcaaccc
    12421 atctttctga taacgctgtc gatttccgcg aaattgatta cactcgcccg acctgcattt
    12481 tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt ggccctggcg gatcaggaca
    12541 tcatcattcc gatgatcggc atggtgcagt cgctgaatgt ttccgttgcc tcagccctca
    12601 ttctttacga agcccagcgt cagcggcaaa atgcaggcat gtacctgcgt gaaaacagca
    12661 tgttgccgga agcagagcaa caacgcctgt tgtttgaagg cggctatccg gtgctggcga
    12721 aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa tcagcaaggc gagatcgaag
    12781 ctgatgccga ctggtgggct actatgcagg ctgcagggta agtgccatga aaggtcgcct
    12841 gttagatgct gtcccactca gttccctaac gggcgttggc gcagcactta gtaacaaact
    12901 ggcgaaaatc aacctgcata ccgtgcagga tctactctta caccttcccc tgcgctacga
    12961 agatcgcacc catctctacc ccatcggaga actactgccg ggcgtttatg ccacggtgga
    13021 aggcgaagtg ctgaactgca atatctcctt cggcggtcgg cggatgatga cctgccagat
    13081 cagcgacggt tccggcatcc tcaccatgcg ctttttcaat ttcagcgcgg caatgaaaaa
    13141 tagcctggcg acgggccgcc gtgtactggc ttatggcgaa gcaaagcgcg gtaaatatgg
    13201 tgcggagatg atccacccgg aataccgcgt gcagggcgat ctcagcacgc cagaattaca
    13261 ggaaacgctc acgccggttt atccaacaac ggaaggcgta aagcaggcca cgctgcgtaa
    13321 attaaccgac caggcgctgg atctgctcga cacctgcgcc attgaagaac tcctgccgcc
    13381 ggaactgtca caaggaatga tgacgctacc ggaagcgttg cgcactttgc accgcccgcc
    13441 accgacgcta cagcttagcg atctggaaac cgggcagcat ccggcgcaac gtcgtctgat
    13501 tctggaagaa ctgctggcgc acaacctcag catgttagcc ttacgtgccg gagcacagcg
    13561 ttttcatgcc cagccgctga gcgccaatga tgcgctgaaa aataaactcc tcgcagcctt
    13621 accgttcaag ccaacgggcg cacaggcacg cgtagtggcg gagatcgagc gcgatatggc
    13681 gctggatgtg ccgatgatgc gcctggtaca gggcgatgta ggttccggta aaacgctggt
    13741 cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa caggtggcat tgatggcacc
    13801 aaccgaatta ctcgccgaac agcacgccaa taacttccgc aactggtttg aaccgctcgg
    13861 tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa gcgcgactgt ctcagcagga
    13921 agccatcgcc agcggtcagg tgcagatgat tgttggtacg cacgccatct tccaggaaca
    13981 ggtgcagttt aacggtctgg cgctggtgat tatcgacgaa caacatcgct ttggcgtgca
    14041 tcagcgtctg gcgttatggg agaaaggcca gcagcaaggc ttccatccgc atcagttgat
    14101 catgaccgcc acgccgatcc cccgcacgct ggcaatgacg gcgtatgccg atcttgatac
    14161 ctcggtgata gatgaactgc cgccaggccg cacgccagtg actacggtcg ctattcctga
    14221 tactcgccgt accgacatca ttgaccgcgt gcgccacgcc tgcatgactg aaggtcgtca
    14281 ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta ctggaagcgc aggcggcgga
    14341 agctacctgg gaagagttga aactggcgct accagaattg aatgttggcc tggtacacgg
    14401 gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg tttaaacaag gtgagctaca
    14461 tctgctggtt gccactaccg ttattgaagt cggtgttgat gtgcctaacg ccagcctgat
    14521 gattatcgaa aaccctgagc gtctgggtct ggcgcagtta caccagctgc gcgggcgcgt
    14581 aggtcgtggc gcggtggctt ctcactgcgt gctactctac aaaacgccgc tttctaaaac
    14641 ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac ggttttgtga ttgcgcaaaa
    14701 agatctggag attcgcggcc ctggcgaatt gttaggcacg cgtcagacgg gtaatgctga
    14761 atttaaagtg gcggatttac tgcgcgatca ggcgatgatc ccggaagttc agcgcctggc
    14821 acgccatatt cacgaacgtt acccacaaca ggcaaaagcc ctgatagaac gctggatgcc
    14881 ggagacggaa cgttactcga atgcgtaaaa ggcggcagtg agaagaccgc catttcaggt
    14941 taccctacct tcctgccgga tgcgattcat caccctacaa attcaataaa ttatgaatca
    15001 atacgcaggc ttgataagcg aagcgtatca ggcatttgtc tttgttgcgg cggcacttct
    15061 accgccgctt catcggttaa ccggcaaaaa tcggcaacat caaatacaac ttaattacca
    15121 gcgcattgac gatatcgata aagaacgcac cgaccatcgg caccaccaaa aacgccatgt
    15181 gcgacgggcc aaagcgttca gtaatcgcct gcatgttggc gattgccgtt ggcgttgcac
    15241 cgaggccaaa accacagtga cctgcagcca gcactgccgc atcgtagttt ttgcccatca
    15301 tgcgccaggt aacgaagatg gcatacaacg ccatgaagat ggtctgtacc accagaatcg
    15361 ccagcatcgg cagcgccagc gaagccagct cccacagttt cagccccatc aacgccatcg
    15421 ccaggaacaa cgacaagctt acgttaccca gcacggatac cgcacgctca aagacgcggt
    15481 aaaagcccat tattgacaga ccgttgctca gaatcacgcc aacaaacagt acacagacga
    15541 aggtcggcag ttcaaaagca gtgccagcca aaagttgcgc aacaattttc cccaccgtca
    15601 ggcagatagc aatcagcgcg atagtttcaa tcagcaccaa cgaggtgatc atgcgtccca
    15661 catccggctt ttcaaacgcc gtcgggactt cctggtcatc cggaataccg ttcggcgtgg
    15721 tggagtgttt caccagatag cgcgccaccg gaccgccaat caagccgccc agcaccagac
    15781 cgaacgttgc acaggccatc gccacttccg tcgcattggt gaagccataa cgttcaatga
    15841 acaatttact ccacgcagcg cccgtaccgt gaccgccgga aagcgtaata gaaccggcca
    15901 acagccccat cagcggatca agccctaaca ggctagccat accaatgcca atggcatttt
    15961 gcatcaccaa cagaccaaca accacaatca agaagatgcc aaccacacgc ccaccggcac
    16021 gcaaactggc aatgttggcg ttcaggccaa tggtggcgaa gaaagccagc attaacggat
    16081 cgcgcaggga catatcaaag ttgacttccc agcccatgct ttttttcagt actagtagcg
    16141 ccagcgccac caacaaacca cccgcaacag gttccggtat ggtgtatttc ttcaaaaagg
    16201 agacggaatg gaccaactta cgcccgagca gcaacgtcag cgttgcggca acaagcgttg
    16261 ctaaagtatc gagatgaaac atagttactc ctttgtatcc gcatcactct ttcatactcg
    16321 tcatacttca tcgcgcagtg gcaacttcat gaactatgac gggtatcgag ttatgctttc
    16381 ctgggtcaaa ttctcagctg ttaatgaaca aatggcatgg attttaagca gaaaaatacc
    16441 aaaagttata taaaaaagtg catttatctc atttaaatat ttagatgagg atgtggcaaa
    16501 cgtttgcttt tctctttcgg tcagctaaaa tgcccgcttt gctaccacgg gattgttttc
    16561 gatgtctgtt tccaccctcg agtcagaaaa tgcgcaaccg gttgcgcaga ctcaaaacag
    16621 cgaactgatt taccgtcttg aagatcgtcc gccgcttcct caaaccctgt ttgccgcctg
    16681 tcagcatctg ctggcgatgt tcgttgcggt gatcacgcca gcgctattaa tctgccaggc
    16741 gctgggttta ccggcacaag acacgcaaca cattattagt atgtcgctgt ttgcctccgg
    16801 tgtggcatcg attattcaaa ttaaggcctg gggtccggtt ggctccgggc tgttgtctat
    16861 tcagggcacc agcttcaact ttgttgcccc gctgattatg ggcggtaccg cgctgaaaac
    16921 cggtggtgct gatgttccta ccatgatggc ggctttgttc ggcacgttga tgctggcaag
    16981 ttgcaccgag atggtgatct cccgcgttct gcatctggcg cgccgcatta ttacgccgct
    17041 ggtttctggc gttgtggtga tgattatcgg cctgtcgcta attcaggtgg ggctgacctc
    17101 cattggcggc ggttacgcag ccatgagcga taacaccttc ggcgcaccga aaaatctgct
    17161 gctggcaggc gtggttttag ccttaattat cctgcttaac cgtcaacgta acccttactt
    17221 acgcgtggcc tcactggtaa ttgcgatggc ggccggatat gcgctggcgt ggtttatggg
    17281 catgttgcca gaaagcaacg aaccgatgac gcaagaactg attatggtgc caacgccgct
    17341 ctattacggt cttggcattg aatggagtct gctgctgccg ctgatgctgg tctttatgat
    17401 cacttcgctg gaaaccattg gcgatatcac ggcgacctct gacgtttccg aacagccagt
    17461 gtccggtccg ctgtacatga aacgcctgaa aggcggcgtg ctggcaaacg gcctgaactc
    17521 gtttgtttcg gcggtgttta acaccttccc gaactcctgc ttcgggcaaa acaacggagt
    17581 gatccagttg accggtgttg ccagccgcta tgtcggtttt gtcgtcgcgc tgatgttgat
    17641 cgtgctgggt ctgttcccgg cagtgagcgg ttttgtacaa cacattccag aaccggttct
    17701 gggcggcgca acgcttgtaa tgtttggcac catcgccgcc tccggtgtgc gtatcgtttc
    17761 tcgtgagccg ctgaaccgtc gggcgattct gattatcgcg ctgtcgctgg cggttggtct
    17821 gggcgtgtct cagcagccgc tgattttgca gtttgcccct gaatggctga aaaacctgct
    17881 ctcctccggg atcgccgcgg gcggtattac tgccatcgtg ctgaatctga ttttcccacc
    17941 agaaaaacag taattcactg gcggcggtaa gataattacc gccgcagtct cacctcttta
    18001 accattcccg ccttgaggat tgcgcgtaaa tcgtgcataa ctcccttatg tgcatttcac
    18061 gggatggaag accatgaaat ttattgggaa gctgcttctc tacattctca tcgctctgtt
    18121 agtggcgatc gctggcctct attttcttct gcaaacccgc tggggagcag aacatatcag
    18181 cgcatgggtt tccgagaata gcgactatca tctggccttc ggggcgatgg ttcaccgttt
    18241 ttccgcgcca tctcatatcg tgctggagaa tgtcacgttt ggtcgtgatg gccagcccgc
    18301 gaccctggtg gccaaaagtg tcgacattgc gctaagtagt cggcaactga ccgaaccacg
    18361 ccatgtcgat accatcctgc tggaaaacgg gacgctgaat ctcaccgacc agaccgcgcc
    18421 gctaccgttc aaagccgatc gtctgcaact gcgtgatatg gcgtttaata gcccgaatag
    18481 cgaatggaaa ctgagcgcgc agcgggtaaa tggcggcgtg gttccgtggt caccagaagc
    18541 cggtaaagtg ctgggtacga aggcgcagat tcagtttagt gccggatcgc tttcgctcaa
    18601 tgatgttcct gccaccaatg tactgattga aggcagtatt gataacgatc gcgttacgct
    18661 gactaacctg ggtgccgaca tcgcgcgcgg gacattaacc ggaaacgcgc agcgtaacgc
    18721 cgacggcagc tggcaagtgg aaaacctgcg catggcggat atccgtctac aaagcgaaaa
    18781 atcgctaacc gacttctttg cgccattacg ctctgtcccg tcgttgcaga ttggtcgcct
    18841 ggaagtgatc gatgctcgtt tgcaaggtcc ggactgggcg gtgaccgacc tcgatctcag
    18901 cttgcgcaac atgaccttca gtaaagatga ctggcagaca caagaaggca aactgtcgat
    18961 gaacgctagc gagttcattt atggttcgct gcatttattt gacccgatta taaacgcgga
    19021 attttctccg cagggcgtag cgctgcgcca gttcaccagc cgctgggaag ggggtatggt
    19081 cagaacgtca gggaactggc tgcgtgacgg gaaaacgttg atccttgatg atgcggcaat
    19141 tgccggactg gaatatacct tgccgaaaaa ctggcaacag ttgtggatgg aaacgacacc
    19201 cggttggtta aacagcctgc aactgaagag atttagcgcc agccgcaatc tgatcattga
    19261 tatcgaccct gacttcccgt ggcagctcac cgcgctcgat ggttacggtg ccaacctgac
    19321 gctggttacc gatcataaat ggggcgtctg gagtggctcg gcgaatctga atgccgccgc
    19381 cgcgacgttt aatcgtgttg atgtccgtcg tccgtcgctg gcgctaaccg ccaacagcag
    19441 cacggtgaat atcagcgaac tgagtgcatt tactgaaaaa ggcattctgg aagccaccgc
    19501 cagtgtttca caaacgccac aacgtcagac acatatcagc ctgaatggac gcggtgtgcc
    19561 ggtgaatatt ttgcaacagt ggggatggcc tgaattaccg ttgactggcg acggcaatat
    19621 tcagcttacc gccagtggcg atattcaggc caatgtcccg ttgaaaccta cggttagcgg
    19681 gcaattgcat gccgtgaacg ccgcaaagca ccaagtgact caaaccatga atgcgggcgt
    19741 cgtttccagc ggtgaagtta catcggcaga gctggtgcag taaacatcaa tctcgccggg
    19801 tacgactcag acgtacccgg cattcttcaa tagattacaa cgtaattgtc agcgcattcc
    19861 cttgaggcgt aaccaccagc ccctgctcac tttcagcctg cgaaccgcct tgcagaccat
    19921 ttactttcac aatattgcgc aagcacagtg tccagttcct cgcctcgccc gcaccagtca
    19981 cagtaatcgt gttaccagta cgtgttgctt ttagagtaaa gatcaccgaa ccgtcagcag
    20041 cgggcacttc acatacagct tcatgcccgt cttgcagatt aaagagatgg aatgccgtgc
    20101 cttcgtgcca cgcgtaatcg ggacgttgat cgttgttgcc cagcgccaat agggtgttat
    20161 cacgcacata aacgggcaga ctcaggaagc cgtgctgctg tttatgccag cgactaccgt
    20221 cgagctcatt gttgtgccac aggtgtgtcc agcgaccttc cggcaagtag aactgcacct
    20281 cgcccgcctc agtgaacacc ggagcaacca tcacgttgtc gcctagcatg tattgacggt
    20341 caaggtaatc acaagccgga tcgtccggga actccatcat catggcccgc atcatcggcg
    20401 taccccgcgc tttcgcacgc gcagcttcac gatacagata cggcatcatg cggcatttca
    20461 gttgcgtgaa gaagcgcacc acatcacagg actcatcatc gtaggcccac ggcacacgat
    20521 aagatttgct gccatgtaaa cgactatggc tggagagcaa accaaacgcg caccagcgtt
    20581 tgtaaacgtg tgccggcgcg gtattttcaa agccgccgat atcgtggctc cagaagccaa
    20641 aacctgaaag gccaatagac aaaccaccgc gcaggctttc cgccattgat tcgtagttag
    20701 cgtaacaatc gccaccccag tgtaccggga atttctgcgc accgacggag gccgagcggg
    20761 caaacaagac agcttcttcc tcaccaacgg tgtccttgag cacgttccac accagttcgt
    20821 tgtagatgta cgcataatgg ttatgcattt tctgcggatc ggaaccgtca aaccactgaa
    20881 catcagttgg gatacgttcg ccaaagtcgg tcttaaagca atcaacgccc atcgcgacca
    20941 gacctttcag tttgtcggcg taccatttgc aggcatccgg attggtaaag tcataaatcg
    21001 ccaga
//
LOCUS       DAZPLI010000065        20429 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZPLI010000065 DAZPLI010000000
VERSION     DAZPLI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.066"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(50..475)
                     /gene="nudI"
                     /locus_tag="ACHUU3_002274"
     CDS             complement(50..475)
                     /gene="nudI"
                     /locus_tag="ACHUU3_002274"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR2082322.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            754..1296
                     /locus_tag="ACHUU3_002275"
     CDS             754..1296
                     /locus_tag="ACHUU3_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR2082323.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            1396..2598
                     /locus_tag="ACHUU3_002276"
     CDS             1396..2598
                     /locus_tag="ACHUU3_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR2082324.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2818..3600
                     /locus_tag="ACHUU3_002277"
     CDS             2818..3600
                     /locus_tag="ACHUU3_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFR2082325.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            3615..4820
                     /gene="rhmD"
                     /locus_tag="ACHUU3_002278"
     CDS             3615..4820
                     /gene="rhmD"
                     /locus_tag="ACHUU3_002278"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFR2082326.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAVMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4877..6166
                     /locus_tag="ACHUU3_002279"
     CDS             4877..6166
                     /locus_tag="ACHUU3_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2082327.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6184..6987
                     /gene="yfaU"
                     /locus_tag="ACHUU3_002280"
     CDS             6184..6987
                     /gene="yfaU"
                     /locus_tag="ACHUU3_002280"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFR2082328.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(7028..7930)
                     /locus_tag="ACHUU3_002281"
     CDS             complement(7028..7930)
                     /locus_tag="ACHUU3_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR2082329.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8123..9313)
                     /gene="glpC"
                     /locus_tag="ACHUU3_002282"
     CDS             complement(8123..9313)
                     /gene="glpC"
                     /locus_tag="ACHUU3_002282"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR2082330.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(9310..10569)
                     /gene="glpB"
                     /locus_tag="ACHUU3_002283"
     CDS             complement(9310..10569)
                     /gene="glpB"
                     /locus_tag="ACHUU3_002283"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR2082331.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10559..12187)
                     /gene="glpA"
                     /locus_tag="ACHUU3_002284"
     CDS             complement(10559..12187)
                     /gene="glpA"
                     /locus_tag="ACHUU3_002284"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR2082332.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12460..13818
                     /gene="glpT"
                     /locus_tag="ACHUU3_002285"
     CDS             12460..13818
                     /gene="glpT"
                     /locus_tag="ACHUU3_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR2082333.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13823..14899
                     /gene="glpQ"
                     /locus_tag="ACHUU3_002286"
     CDS             13823..14899
                     /gene="glpQ"
                     /locus_tag="ACHUU3_002286"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR2082334.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(14941..15183)
                     /locus_tag="ACHUU3_002287"
                     /pseudo
     CDS             complement(14941..15183)
                     /locus_tag="ACHUU3_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            15178..15282
                     /locus_tag="ACHUU3_002288"
                     /pseudo
     CDS             15178..15282
                     /locus_tag="ACHUU3_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15362..16011
                     /gene="inaA"
                     /locus_tag="ACHUU3_002289"
                     /pseudo
     CDS             15362..16011
                     /gene="inaA"
                     /locus_tag="ACHUU3_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(16065..16319)
                     /gene="yfaE"
                     /locus_tag="ACHUU3_002290"
     CDS             complement(16065..16319)
                     /gene="yfaE"
                     /locus_tag="ACHUU3_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR2082335.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(16319..17449)
                     /gene="nrdB"
                     /locus_tag="ACHUU3_002291"
     CDS             complement(16319..17449)
                     /gene="nrdB"
                     /locus_tag="ACHUU3_002291"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR2082336.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(17683..19968)
                     /gene="nrdA"
                     /locus_tag="ACHUU3_002292"
     CDS             complement(17683..19968)
                     /gene="nrdA"
                     /locus_tag="ACHUU3_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR2082337.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            20096..20239
                     /gene="ypaB"
                     /locus_tag="ACHUU3_002293"
     CDS             20096..20239
                     /gene="ypaB"
                     /locus_tag="ACHUU3_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR2082338.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         4759 a         5004 c         5528 g         5138 t
ORIGIN      
        1 ttaaccacta aattttcaat ctgacagcca gtgatggctg tcatcattgt taaagaagac
       61 ctttcaaacg taacgttttt cgggtggcaa catttaaatc ataatgcacc agatcttcag
      121 gttttaccca cgcgtagtcc tgaaactctt cgtttatttt cacttctcgg ttggcagaaa
      181 cgcagtcaaa aatcaggtaa atcatataaa tctcttcctt gcgaccatct gcatacgtct
      241 tggtgcgaat atcatcgctg aaggtccacg gcgtgatttc tgtcaaaagc agctgttctc
      301 ccagttcttc gcgaatttcg cggcgtagtg cctcttcaat tcgttcgcca gactccacgc
      361 cgccacccga aagcgcccat tgaccgggga aaacgccgcg atcgtcggcc attttgcaca
      421 gcaaataagc accatcattt tgaatcaaag ggcatacaat agtccgttgt cgcacagggg
      481 tattccttat aaataaaaag aaaccatgag aatacggtaa ctcagaaata tgtgaaagtg
      541 acagtaatct cttttgcgat gataactgca cttattatta tttttgtgtg tatcggtaaa
      601 gtgtgtttat tttttcggcg tttaattatt taagaagtat ttagttacaa taagtgacta
      661 gatccctttt ttcgctcatt gaatcctctg tgccaggcag tacaatcccg cagctttggg
      721 aattttgatt aattgcttgt gggaaagggg ataatgaaaa aaattgcgct agcaggtctg
      781 gccggaatgc tgttggtttc tgcatcggtc aatgcaatga gcatcagcgg tcaggcgggt
      841 aaagaataca ccaatattgg tgtcggtttt gggactgaat cgacgggcct ggcgttaagc
      901 ggtaactgga cacataacga cgacgacggt gacgtcgctg gcgtggggct ggggttgaat
      961 ctgcctctcg ggccgttaat ggcgaccgtt ggcggaaaag gcgtttacac caacccgaat
     1021 tacggcgatg aaggttatgc cgcagcggta ggaggtggtt tgcagtggaa aattggcaac
     1081 agcttccgtt tgtttggcga gtattactac tctccggatt cactctccag cggtattaaa
     1141 agttatgagg aagcgaatgc tggcgcgcgt tacaccatta tgcatccagt cagtattgag
     1201 gcgggttatc gctacctgaa tctgtcgggt aaagacggta accgcgacaa cgccgtggct
     1261 gacggcccgt atgttggggt taacgccagt ttctgatccc ttccggcgcg gcattctgtc
     1321 gcgccattca gtttcttact acgccttatc tccctttgcg ttatagtgat tcgataacaa
     1381 aagcaggaga taaccatgtt aaaagtggaa atgttatcca ccggggatga agtgttacac
     1441 gggcaaatcg ttgacactaa cgctgcctgg ctggccgatt ttttctttca tcaggggttg
     1501 ccattatctc gccgcaatac ggtgggggat aaccttgatg acttagtcac cattcttcgc
     1561 gaacgtagtc agcacgccga tgtgctgatc gttaacggcg ggctgggacc gaccagtgat
     1621 gatttaagtg cactcgccgc tgcgacagca aaaggtgaag gcctggtgct gcatgaagcc
     1681 tggctcaaag agatggaacg ctatttccac gaacgtggac gagtaatggc accgagcaac
     1741 cgtaaacaag cggagctgcc tgccagtgct gaatttatca ataacccggt aggcaccgcc
     1801 tgtggttttg ccgtgcggct taatcgttgc ctgatgttct ttactcccgg cgtaccgtca
     1861 gaatttaagg tgatggtcga gcacgaaatc ctgccgcgcc tgcgcgagcg tttttcttta
     1921 ccacagtcgc cggtttgtct gcgtttaact acctttggtc gttcggaaag cgatctggca
     1981 caaagcctgg acactctaca actgccgccg ggtgtaacaa tgggctatcg ctcctcaatg
     2041 ccgatcatcg aactgaaact caccggaccg gcaagcgagc aacaggcgat ggaaaaactg
     2101 tggctggacg ttaaacgtgt tgccggacag agcgtgattt tcgaaggcac cgaaggactg
     2161 cccgcgcaga tcagtcgcga attgcaaaac cgccagttca gcctgacgtt gagcgagcaa
     2221 ttcaccggtg gtttattggc tttgcaactt tctcgcgcag gtgctccatt gctggcgtgt
     2281 gaagtggttc cttcacagga agaaaccctg gcgcaaactg cacactggat tacagaacgg
     2341 cgggccaacc attttgccgg gctggcactg gctgtttcgg gtttcgagaa cgagcatctc
     2401 aactttgcgc tagccacgcc agacggcact ttcgctctgc gtgtgcgttt cagcactaca
     2461 cgctacagcc tggctatccg tcaggaagtg tgcgcaatga tggcactgaa tatgctgcgc
     2521 cgttggttaa acggccagga tatcgccagt gagcatggct ggattgaggt tgttgagtcc
     2581 atgaccttat ctgtctgatt ccgccactct gtgccggttt actgcaccgg cacattcccc
     2641 ctccggcaaa acttgtccaa cctgttgtac tcaataagaa aaaatccgtg cactgattca
     2701 caaatttgtg cgtaatttct ccagtccgct ggacgaggag cgggatttga tgcgcttcac
     2761 aattttactc cccctgagca acgagactga ttccagtatc taagactgga gtccagtatg
     2821 ttggaatcaa gcaaagttcc cgccctgaca cgggctattg atattctcaa tttgattgcc
     2881 cgcattggcc cctgtagtgc tgcgacgatc attgacacat tgggaatccc taaaagcacg
     2941 gcctatttgc tgcttaatga actcaggcgt cagcgttttc ttagccttga tcaccaggaa
     3001 aatttttgtt tgtggaccag gctggtggag ctttctggtc atgcgttaag caagatggat
     3061 ctccgggaac tggctcgacc gcgtttgacg caactgatgg ataccaccgg gctgttatgt
     3121 cacctgggga ttattgataa cggcagcgct tattacattt taaaagtgga gtcatcagcc
     3181 actatcagcg tgcgttccca tgaaggaaaa agcctttcgc tttatcgctc cggaataggt
     3241 aaatgcctgc ttgcctggca acctgcagcg gtacagcaaa gtattatcga agggctggtc
     3301 tgggagcagg cgacacccac aacgattact catccacagc aacttcacga agaactggcg
     3361 cgtattcgcc gtcagggctg gagctatgac aacggcgaag attaccccga tgtccgttgt
     3421 gtcgctgcgc cggtgtttaa cgccaataac gagcttaccg ctgcgatctc ggtggtcggt
     3481 acccgcttac aaatcaatga agaataccgt gattatctgg cgggcaaggc cattgcctgc
     3541 gccagggata tttcacgtct gttgggatgg aaaagtccct ttgacttaca ggcctcataa
     3601 caatggagaa catcatgacc ctaccaaaaa tcaaacaggt tcgcgcctgg tttactggcg
     3661 gtgcgacagc agaaaaaggt gctggcggcg gtgattatca cgaccagggg gcgaatcact
     3721 ggattgacga tcatattgcc accccgatga gtaaataccg cgattacgag cagtcacgcc
     3781 agtcatttgg cattaacgtt cttggcacgt tgattgttga agtcgaagca gaaaacggcc
     3841 agaccggatt cgctgtttcg acagccggtg aaatgggctg ttttattgtc gaaaaacatc
     3901 ttaaccgttt cattgagggt aaatgtgtca gtgatatcaa actgatccat gatcaaatgc
     3961 tcaatgccac cctgtattac tccggctctg gtggcctggt gatgaacacg atttcctgtg
     4021 tcgatctggc tctgtgggat ctgttcggca aagtggtcgg gcttccggtt tataaacttt
     4081 taggcggcgc agttcgtgat gagattcagt tctacgccac aggcgcgcgt ccggatctgg
     4141 caaaagagat gggctttatc ggtggcaaaa tgccgacgca ctgggggcca catgatggcg
     4201 atgcggggat ccgcaaagat gccgttatgg tcgcggatat gcgtgaaaaa tgcggtgagg
     4261 atttctggtt aatgctcgac tgctggatga gtcaggacgt gaactatgcc accaaactgg
     4321 cccacgcttg cgcgccctat aacctgaaat ggatcgaaga gtgcctgccg ccacagcagt
     4381 atgaaggtta tcgcgaactg aaacgcaacg cgccagtcgg aatgatggtc accagcggtg
     4441 agcaccacgg cacactgcaa tcttttcgta cgctttcaga aaccggtatc gacattatgc
     4501 agccggatgt tggctggtgc ggtggtttaa ccacgctggt ggaaattgcc gcaatcgcca
     4561 aatccagggg gcaactggtg gtgccgcacg gttcgtctgt ttactctcac catgcggtga
     4621 tcaccttcac caatacgcca ttcagcgaat tcctgatgac cagcccggat tgttcaacga
     4681 tgcgtccgca gtttgacccg attctgctca atgagccggt tccggtgaat ggtcgtattc
     4741 ataaatcagt gcttgataaa cccggttttg gcgtcgaact caatcgtgac tgcaatttga
     4801 aacgccccta cagccactaa tcactccggg cgttgctgtg acaacggtaa cgcccacata
     4861 ttctaaggag ctttttatga gcaccgcttt gcttgacgcc gtggtgaaga aaaaccgtgt
     4921 gcgtttaatt ccgtttatgt tggcgctgta tgtgctggcg tttctcgacc gttcgaatat
     4981 cggttttgcc aaacagacct accagattga taccgggttg agtaatgaag cttatgcgct
     5041 gggagcaggc attttctttg tggtatatgc gtttctgggt gttccggcga atcttttgat
     5101 gcgcaaactg ggggccagaa cctggattgg tacgacaaca ctgctgtggg gatttctttc
     5161 ggcagccatg gcatgggccg atactgaagc gaaatttctg atagttcgca ctctgcttgg
     5221 tgctgcggag gccggatttt tccctggtat gatttatctc acctcgcaat ggtttccgca
     5281 gcgtaatcgc gccagcatta tggggctgtt ctatatgggc gcaccgctgg cgttaacact
     5341 gggatcaccg ctttccggcg cgctgttgga gatgcatgga tttatggggc atcccggctg
     5401 gttctggatg tttgtgattg aaggattgtt ggcagtcggt gctggggtat tcacattctt
     5461 ttggcttgat gacacaccgg agcaggcacg ttttctgagt aaacaagaaa aaacgttgct
     5521 tatcaatcaa ctggcaagtg aagaacaaca gaaagtgact tctcggctga gcgatgcgct
     5581 gcgtaatggc cgagtctggc aactagcgat tatctacctg accattcagg tggcggttta
     5641 cggattaatt ttcttcctgc cgacccaggt tgcggcattg ctgggaacaa aagtgggctt
     5701 tacagcgtcg gtggtcaccg ccattccgtg ggttgcggcc ttgtttggga cctggcttat
     5761 tccgcgctat tccgataaaa ccggcgaacg gcgtaatgtc gcagcgctga cattactggc
     5821 ggcgggcatt ggtattggtc tgtccgggct gctttctcca gtaatggcga tcgtagcgct
     5881 gtgtgttgca gctatcgggt ttattgccgt gcagccggtg ttctggacga tgccgacaca
     5941 actgctttcc ggtacggcgc tggctgcggg gattggtttt gtaaacctgt ttggtgcagt
     6001 gggcgggttt attgccccga tcctgcgcgt gaaagcagaa acgttatttg ccagcgatgc
     6061 ggcaggatta ctgacactgg cagcggtggc ggtcatcggt tcgctgatta ttttcactct
     6121 gcgtgtaaat cgtactgttg cgcagaccga cgtggcacat cattaaatag gttaaggaac
     6181 acgatgaacg cattattaac caatcccttt aaagaacgtt tacgcaaggg cgaagtgcaa
     6241 attggtctgt ggttaagctc gactacttct tatatggcag aaattgccgc tacttccggt
     6301 tatgactggt tgctgattga cggggagcac gcgccaaaca ccattcagga tctttatcac
     6361 cagctacagg cggtagcgcc ctatgccagc caacccgtga tccgtccggt ggaaggcagt
     6421 aaatcgctga ttaaacaagt tctggatatt ggcgcgcaaa ctctattgat cccgatggtc
     6481 gatactgccg atcaggctcg tcaggtggcg tctgccacgc gctatcctcc ctacggtgag
     6541 cgtggtgttg gggccagtgt ggcacgggct gcgcgctggg gacgcattga gaattacatg
     6601 gcgcaagtca acgattcgct ttgtctgtta gtgcaggtgg aaagtaaaac ggcactggat
     6661 aacctggacg agatcctcga cgtcgaaggg attgatggcg tgtttattgg accagcggat
     6721 ctttctgcgt cgttgggcta cccggataac gccgggcacc cggaagtgca gcgaattatt
     6781 gaaaccagca ttcggcggat ccgtgctgcg ggtaaagcgg ctggttttct ggctgtggct
     6841 cctgatatgg cgcagcaatg cctggcgtgg ggagcgaact ttgtcgctgt tggcgttgac
     6901 acgatgctct acagcgatgc cctggatcaa cgactggcga tgtttaaatc aggcaaaaat
     6961 gggccacgca taaaaggtag ttattgatat caaaggccca tggggatcgg ctgtgggcct
     7021 gtgttaatta gtggttattc gctgccagat cggcttcgct tagctgggtg gccgcgagca
     7081 cctggtcacg atcaatgccc tgttccagca tgcgcaaagc aattttaatg gcttgttcat
     7141 gcataccttc ctgccagcca atttggtgtc cttcctgccg tagccgctca gcaatagtca
     7201 tcaatctctc cttttgtagt ggtgaacgtt cggaaatctc ctcaataaaa cgggtgaaac
     7261 gggaggtatc gccgcattgc agcagataat taaacagtgt ttgtagctgg ctgtcattag
     7321 tgaagccttt aaccaaaagc gtggtgatcc tgtcgaccat accgattaaa tcgcggtcgc
     7381 gaatatgctt ttgaatcagt tcaagcagag ctatacgccg atgttgcatg atctcatcgt
     7441 caggtacgat ggtgatatcc accaacggaa aagcttcggt gtacaactgc cgggcaagtt
     7501 gcggatcgtc aaactcatcc agccagttaa gtgagtacgg gtagggcgat gtttcgccat
     7561 gataaaacag caatggcacc accagcggaa ctctgtcata gcctttatcc aggtgacgct
     7621 gcatggcggc agtggcatag cgcattagcc gaaaagccat attcttttct gcagagcttt
     7681 gatgttcaat cacgcagtag atatagccgt caccgtcgct ggtttccacg gaccacaaaa
     7741 catccgagta gtaagcgcgt aaactttttt caataaaact agtgggttcc aggcgtaagg
     7801 tttgcaggtt gcaaagcttg cgcagtggtt ctggcaaatg tatttcgaga aaatcccgtg
     7861 cggtttcggg tgtgaacata aaggttttaa ataccgcatc atgcggggag gaggttgttg
     7921 attcggtcat ggttgtccgt cacctgatat ttatgatgac gcgacattac ggttatccca
     7981 ggcctttgac atctgccagt ttcagccttt gcgagacgtt ttcagaggaa ttttcactgc
     8041 gttgcaacgc atacattggg cacggaatcg tgtacgcaaa aatgcccggt aaccacatgc
     8101 ttaccgggca tcagaaagga gtttaagcca gcgcctgggc cagtagcgta atcggatgtt
     8161 cgcagcgaag acttgtggac atctcaatct gccatttaca ggtttcgcag tcggtgacca
     8221 ccagatctgc gccgctttct tctatctggc ggaacagtgg tgcgccgatg gcttgtgagg
     8281 tggggtagtt ctcttttttg aaaccgtaag tacccgcaat accgcagcac tgggaatcca
     8341 gcaccgttaa ctcaagcccc gggattttac gcaacagctc cagggtgtag agcgtccagc
     8401 ccattttttc catatggcac ggagtgtgat aaaccacttt cagcggcagc ggtttcagcg
     8461 gtaacgtttt gccttcgtcc agcttgcgcc acagccagcg ggttgccagt tcgatatgat
     8521 cgcgcaagcc tttgttgtcg acattcagca cttccgggta ttcgtcgcgc agggcaaatg
     8581 tacaggttga ggaggtggca atcaccggaa tgccttttac tcccacagct tcgcggatcg
     8641 actctacatt cgtaattgcc tgtttgcgtg ctttatcggt aaagccgttg gcgattagcg
     8701 gtacgccgca gcatttttct ttgctgagca gttgtacacc gatacccatt gcgttgagca
     8761 ctttaattaa atctttacct aactgcggat ggttgtagtt aacgaagcag ccgtgaaaga
     8821 aagcgacctg gtctttatat tgtgcttgct gagccgctat gctgcgatac cagcgacgga
     8881 acgtgccgaa ggagtatttc ggtagcgtgc gacgatgatc gatttttaac gccgcatcaa
     8941 gcagctgccg caccggtttc agcgaggtag cggtgttgac gattggtgcg aacggcgtgg
     9001 aaacgctacc catcaggtcg gtatgactca acacaaaatt acgcagcgac gggcgcgtgg
     9061 tgtcatattt tgcccgcgcg cgctggataa tatcgccaat cttcacatcg gacgggcagg
     9121 cgacttcaca acgtttgcag ttgatgcaat atttcagcgc ctcgtcatac agtgcgccat
     9181 ctttcaaacg cagacgctcg ccatccggcc cggcttgttt tggccctgga taaccgggat
     9241 tcacccggct caccgggcag gcggtggtgc agacggtgca cttaatgcag ttttcgaagc
     9301 tggtgtcgtt cattgttggc ctcctgcgcg ttgggcaatc tgttgagcgg catgtaaagc
     9361 actgacggca caaacaccgc cgccgcatcc ctgggcgatg ggatcaaatc cgcccagcac
     9421 cgaaccgatg gcaaacaggt tctcaatggt ttgccctgcc tgcgacgggc gtagcgtctc
     9481 atcagtggtt acaccgaact gctgccacgg ttgcggcgca aaaaaatcgc ccttatacca
     9541 ttcaccccgc gtggcggttt gtagcacatc aaggccgaga atcggctcgc gaatgccgtt
     9601 acgttccgct accagtccgc cactaaagaa actgccgctg gcgagaaccg cgaaacgtgg
     9661 acgtagcgga atatcggcgt gattacgggt ccagatttca ttcactacgc catttttaca
     9721 ggtcactttt ttcacttcat cgcccggcat ccacacgcca ccctggcgca caaactggcg
     9781 ctgtaactgg ttttgcagac gaatgcccag cacggaaggc ggcagcgttg gcaaaagcat
     9841 cagtgaacaa ggtagttttt cattcaacca acgccacagt ttgtcatcgg ccaggccgaa
     9901 gcaggcgggc atcaggatca tttcgcaggt attggcgaca ggaataagcg catcaagtaa
     9961 cagcggccag ttttcttcat tatcaaggaa acgggcgata ttcaccgcgc gaaattcggt
    10021 ggcgttattg cgcaggacat ccagttccgg cagctctatt tctgcggttt caaccgaaag
    10081 atccagttcg cgcaacgaag ccgccgcaag atgcgcctgg aaatccatca gcccgcttat
    10141 ccccactaca catatcttct tcgcgggtag cggccagacg ggaacttctg gcgaacttaa
    10201 ccaggttgca cgcaaagtgc cgagcggggt aacgcgctga tgggccagtt ccacgctgcc
    10261 ctgcaattgc gcaccgcttt cagcgatcaa tgcctgcgcc tggcaagcga gatcgagcac
    10321 gcgttgtggt tcgagaagac tgtaaggatg ggctggtgcc tgctgacgca aagattccag
    10381 tccactgtga atgtctgtca ccggttgacc atcaggcaga tggctcagca aatccagcga
    10441 accggatgag aaatgcagcg cgctttgacc acgcgtgaca atggcacagc gcaggccgtg
    10501 tttttgcagt tgcaggccac agagtaatcc ggcgaggccg ccgcccataa tgacagtatc
    10561 aaagcgcatc tttctgctcc ttctccagac cacacagtcc ctgataaacc cagcgggtaa
    10621 attcgctttc gcgcagtgca tctccccagg cgatgggttg cacgcctttc cagcgttcgt
    10681 taaggaaggt ggaaagttgc tcgatagatt gcgcggacgt cgtgacgtta aaacgttgca
    10741 gcagtccggc agcgcggcag gcgcagagtt cgccctggca ggtacccatc cccacacggg
    10801 tacgacggcg taaatccagc aggctattaa cgtttaaatt ttctaccgcg tactgcactt
    10861 cacccgctgt taccgcttcg cactcacata ccaggctacg gtgcagacga ccttcgctca
    10921 gccaggcagg cgtgcgatcg ccatgacgat aaaccgcaga accgcgcagc ggggcaggca
    10981 gggagatgac tttacgcaag gtaacttcag cgggttcctg tgaaccagga agtgccagat
    11041 cggcggtcgt acaggggcgc gtgttgccca gtttgcggca cacggcgtcg gtagcccatt
    11101 cagccatcag ccgataggtc atcagtttgc caccggtgat ggtgataaat ccgtccagac
    11161 catcgcgttc agcatggtcg agcagcacga tgccacggct gacgttacgt ccgctcgggt
    11221 cgtcatcgct ggcaaccagc gggcgcacgc cagaataggc ccgcaaaatg cgcgttttcg
    11281 ccatcacggg ggccagtttt tccccttcac gcagcagaat atcaacctct tctgccgtca
    11341 ctcgattatc gtcaatctcg ttgtaatcaa tacgtaaaga ggtggtgcca atcagcgaaa
    11401 tggtatcgcc aggcaccaga atatcggcgt cggaaggttt acggcagcgg ttgatcacat
    11461 gctggttaat gcggtgatcc atgatcagca gcgatccttt cgccgggaac atgcgaatgc
    11521 gcagatcggc atattcggca atgtgttgcc cccagatccc ggcggcatta accacgacag
    11581 gcgcatgaag ggcctgagtt tcgccggtga gatggttacg tacacgaaca ccgcacaccg
    11641 tcgcgccttc acgaatcagt cccgtgactt catgagcggt aaggataacg gcaccgtgtt
    11701 ctttggcatc cagcatgttt gctgcggtca gacgaaatgg atcaacggtg ccatccggaa
    11761 ctttcaccgc gccaatcaat gccgggttaa cggcaggttc gataatgcgc gcttgctgcg
    11821 ggtctatagc ttctgcgctg atccctgctt cttcgcaggc gcgaataaaa gtggcctgga
    11881 aggagaggtc atcttccggc agggtaataa acaggccgtt ggttggttca acgcagtgac
    11941 gtgcaatgcg tttcaggatc tggttttcac taatgcattc gcgggccgat tccgcatcgg
    12001 ttaccgcata gcgcgcaccg ctgtgtagca ggccgtggtt acgcccggtg gcaccggttg
    12061 cgatgtcgtg gcgctcaacc aaaatcacgc gcagcccgcg cagggcacag tcgcgggcaa
    12121 tcccggctcc cgttgcgccg ccgccaatga taatcacgtc acttgattgc gagtcgcgag
    12181 ttttcattgt tatccctctg aagttcgttt tttaccattt agccatagta aaaacatgaa
    12241 ttgtttgatt tcgcgcatat tcgctcatga ttcgaaagtg aaacgtgatt tcatgcgtca
    12301 ttttgaacat tttgtaaatc ttatttaata atgtgtgcgg caattcacat ttaatttatg
    12361 aatgttttct taacatcgcg gcaactcaag aaacggcagg ttctctcact gaatcaggct
    12421 gttaatcata aataagacca cgggccacgg aggctatcaa tgttgagtat ttttaaacca
    12481 gcgccacaca aagcgcgctt acctgccgcg gagatcgatc cgacttatcg tcgattgcgc
    12541 tggcaaattt tcctggggat attctttggc tatgcggctt actatttggt tcgtaagaac
    12601 tttgcgcttg ctatgcctta tctggttgag cagggattct cacgcggtga tttaggtttt
    12661 gccctttcgg ggatctcgat tgcttatgga ttttcgaaat tcatcatggg ttcggtatcg
    12721 gatcgctcga atccgcgcgt tttcctgccc gcaggtttga ttctggcggc ggcagtgatg
    12781 ttgtttatgg gctttgtgcc atgggcgacg tcgagcattg cggtgatgtt tgtactgttg
    12841 ttcctctgcg gttggttcca ggggatgggg tggccgccgt gtggtcgtac tatggtgcac
    12901 tggtggtcgc agaaagaacg tggcggcatt gtgtcagtgt ggaactgtgc gcacaacgtc
    12961 ggtggtggta ttccgccgct gctgttcctg ctggggatgg cctggttcaa tgactggcat
    13021 gcggcgctct atatgcctgc tttctgcgcc attctggtgg cattattcgc ctttgcgatg
    13081 atgcgcgata ccccgcaatc ctgtggcttg ccgccgatcg aagagtacaa aaatgattat
    13141 ccggacgact ataacgaaaa agcggaacag gagctgacgg cgaagcaaat cttcatgcag
    13201 tacgtactgc cgaacaaact gctgtggtat atcgccatcg ccaacgtgtt cgtttatctg
    13261 ctgcgttacg gcatcctcga ctggtcaccg acttatctga aagaggttaa gcatttcgcg
    13321 ctggataaat cctcctgggc ctacttcctt tatgaatatg caggtattcc gggcactctg
    13381 ctgtgcggct ggatgtcgga taaagtcttc cgtggcaacc gtggggcaac cggcgttttc
    13441 tttatgacac tggtgaccat cgcgactatc gtttactgga tgaacccggc aggtaaccca
    13501 accgtcgata tgatttgtat gattgttatc ggcttcctga tctacggtcc tgtgatgctg
    13561 atcggtctgc atgcgctgga actggcaccg aaaaaagcgg caggtacggc agcgggcttt
    13621 accgggctgt ttggttacct gggcggttcg gtggcggcga gcgcgattgt tggctacacc
    13681 gtggacttct tcggctggga tggcggcttt atggtaatga ttggcggcag cattctggcg
    13741 gttatcttgt tgattgttgt gatgattggc gaaaaacgtc gccatgaaca attactgcaa
    13801 aaacgcaacg gaggctaatg gcatgaaatt gaagctgaaa aaccttagca tggcgatcat
    13861 gatgagcact atagtcatgg gaagcagtgc aatggcggcg gacagcaacg aaaaaatagt
    13921 catcgcccat cgcggtgcca gtggatattt gccggagcat acgctgccag caaaagcgat
    13981 ggcgtatgcg cagggagcgg attatctgga acaggatttg gtgatgacca aagacgacca
    14041 tctggttgtt ctgcatgacc attatctcga tcgtgttact gatgttgccg atcgtttccc
    14101 ggatcgggcg cgcaaagacg gtcgttacta cgcgatagat ttcacgctgg atgaaattaa
    14161 gtcgctgaaa tttaccgaag gtttcgatat tgaaaacggt aaaaaagtac agacttatcc
    14221 ggggcgtttc ccaatgggta agtccgactt ccgggggcac acctttgaag aagagattga
    14281 atttgttcag gggttaaatc actctaccgg gaaaaatatc ggtatctatc cagaaatcaa
    14341 agcgccgtgg ttccatcatc aggaagggaa ggatattgcg gcaaaaacgc tggaagtgct
    14401 gaagaaatat ggttacaccg gtaaggacga taaagtttat ttgcaatgtt ttgatgctga
    14461 tgagctgaag cgtattaaga atgagctgga acctaaaatg ggcatggatc tcaatctggt
    14521 acagctgatt gcctataccg actggaatga aacgcagcag aaacagccgg acggaagctg
    14581 ggttaattac agctatgact ggatgtttaa gccaggtgcc atgaagcaag tcgcggaata
    14641 tgccgacggt atcgggccgg attaccatat gttgattgag gaaacatcgc agccgggtaa
    14701 tatcaaactc actggcatgg tgcaagatgc tcagcagaat aaactggtag tgcatcctta
    14761 taccgtgcgg tcggataaac tgcctgaata cactactgat gtgaatcagt tatatgatgc
    14821 tctgtataac aaagcgggtg taaatgggct gtttactgat ttccctgata aggcagtaaa
    14881 attccttaat aaagagtaat gctactgcag gatggcgttc atgcgccatc ctgtctcata
    14941 tcaaattttt ccaatcgcca ctaattgttc catgcacatt tgtagtaaaa acagtggact
    15001 gccttctacc ggcttttctt tagccaacaa cgaaatctgt cgatagaagg ttggttcgtg
    15061 aggaacggaa cacaattcac ctgcaatggt tttcagcgtt aactctggta ataaggcgat
    15121 gcctaatccc tgtcgaataa aattcatcgc tgtatccacc tgattaaatt cacattttat
    15181 ctgctgggcg cactattagt ggtcgtggct ggtggaatct atgcgctgca aatgaagggc
    15241 aaaagcggtg tcacactagt agcggcaaaa gaaattcatt aatacgacac gtttcattaa
    15301 gattttcctc aggtaaccac ctatagtcat tgtcgttaat aaaaatgaca ggcgataggg
    15361 tatggcagtt tcagcaaagt acgacgaatt taatcactgg tgggcaacag agggcgactg
    15421 ggttgaagag cctaactatc gccgtaatgg tatgagcggg gtgcaatgcg tcgagcgcaa
    15481 cggcaaaaag ctgtatgtaa agcgcatgac gcatcatctg tttcattccg tacgttatcc
    15541 gttcggccga ccaacgattg tccgtgaggt tgcggtaatt aaagaactgg aacgggccgg
    15601 tgtcatcgtg ccgaagatcg tttttggtga agcggtgaaa attgagggtg aatggcgcgc
    15661 gttgctggtg actgaagata tggcggggtt catcagcatt gctgactggt atgcccggca
    15721 tgcagtatcg ccctattctg acgaagtacg gcaagccatg ttgaaagcag tggcgctggc
    15781 gtttaagaaa atgcatagcg ttaatcgtca gcatggctgt tgttatgttc gccatatcta
    15841 tgtgaaaaca gaaggtaaag cagaagctgg ttttcttgac ctggagaaaa gtcgtcgccg
    15901 cttgcgtcgc aataaagcga ttaatcatga cttccgccag ctggaaaata tctggagcca
    15961 atccccaaag cggactggga gcaggtgaaa gcgtattact acgccatgta atcgttgtag
    16021 gccagataag acgtataagc gttgtatctg gcactacaac caattcacat ctcgatttca
    16081 atatcgcctt ttgcccggca acaacagggc aaaatttccc ccggctgaat aaaggctaac
    16141 ggttcggcaa tccagtccac ctgacctgcg accagtcggg tgcgacagga gccgcagtaa
    16201 ccttcgcggc actggtactc aaccgcaaca ttgtgggatt ccagcgccgc cagaagggaa
    16261 gggtgttcat cctggcacag cagttgtgtg ccagtgatgc gcagggtaac gcgggccatc
    16321 agagctggaa gttactcaaa tcgtcggtgt ccacttccgc gtcaatctgc ccgaccagat
    16381 aagaactgac ttccacttcc tgcggagcaa cctgcacgtt atcagacacc agccaggtgt
    16441 tgatccacgg gatcgggttg gagcgcgtct ggaacggcag atccaaaccg actgcctgca
    16501 tgcggatatt ggtgatgtat tcaacgtact ggcagagaat atctttattc agaccaatca
    16561 tcgaaccgtc acggaacaga taatccgccc agtctttctc ttgctgtgcc gcctggacaa
    16621 acaggtcata gcactcctgc ttacattctt cggcaatttc cgccatctcc ggatcatccg
    16681 cgccgctgcg cagcagattc agcatatgct gagtaccggt caggtgcagg gcttcatcgc
    16741 gggcaatcag gcgaataatc ttggcgttac cttccatcag ttcgcgttct gcaaaggcaa
    16801 aggaacaagc aaagctgacg tagaagcgga tcgcttccag cgcgttaacg ctcatcaggc
    16861 agagatacag ttttttcttc agctcgcgca ggctaacggt tacggtttta ccgttaacgg
    16921 tgtgggtgcc ttcgcccaga aggtgccagt agctggtcat ttcgatcagt tcatcgtaat
    16981 agctggagat cccttccgca cgtttctgga tctgctcgtt ggtgacgata tcgtcaaaca
    17041 caacagacgg atcgttaacg atattacgaa tgatatgagt ataggaacgg gaatgaatcg
    17101 tttctgagaa cgcccaggtt tcgacccagg tttccagttc cggaatagaa ataagcggca
    17161 atagcgccac gttcgggcta cgaccctgaa tggaatccag cagcgtctga tatttcaggt
    17221 tgctgataaa gatgtgtttt tcgtgctccg gcagcgcctg gtaatctata cggtcgcggg
    17281 agacgtcaac ttcttccgga cgccagaaga aagagagctg cttttcgatc agcttttcga
    17341 agatgtcata tttttgctga tcgtagcgag ccacgttgac cggctgacca aagaacatcg
    17401 gttctttgag ctgatcattt ttcgtctgtg aaaaggtggt atatgccatg agtgtgtcct
    17461 gttgggagtt taatgccgga taaggcattt tacgccgcat cctgtgcctg atgcgacgct
    17521 ggcgcgtctt atcaggccta caaatccgag ccgtaggccg gataaggcgt tcacgctgca
    17581 tccggcaccc ggagcctgat gcgacgctgg cgcgtcttat caggcctaca aaccgagccg
    17641 taggccggat aaggcgttta cgccgcatcc ggcatctcaa tatcagatct tacatgcgcc
    17701 gctttcgcag ccatcgtcct ggattgacgg taccagatcg tcctgcgcgt cttcagcgcc
    17761 gtcacgggtg ttctgataat acagcgtttt gaccccgaat ttgtaggcgg tgagcaggtc
    17821 tttcagcaac tgctgcatcg gcacttttcc tgacgggaaa cgtgacggat cgtagttggt
    17881 gttagcagag atcgactgat cgataaattt ctgcatgata cctaccagtt gcaggtagcc
    17941 atcgttaccc ggcatttccc acagcagctc ataggcgtcg tgcaggtgct cgtagtccgg
    18001 caccacctgg cgcaaaatac cgtctttcga cgctttgatg ctgacgtaac cgcgcggcgg
    18061 ttcaataccg ttagtggcgt tagagatctg cgaagaggtc tcggacggca tcagggcaga
    18121 aagcgtagag ttacgcaggc cgtgcgtttt gattgattca cgcaatgctt cccagtcgta
    18181 atgcagcggc tcattagcga tggtatccag atctttctta taggtatcga tcggcaggat
    18241 ccctttcgcg taagtggttt cgttaaacca cgggcacgcg ccttgctctt tcgccagctc
    18301 attagaggct ttcagcaggt aatactgaat ggcttcgaag gttttatgcg tcaggttgtt
    18361 ggcgctgccg tcggagtagc gtttaccgtg cttcgccagg tagtaagcga agttgatcac
    18421 accaataccc agcgtacgac gacccatcgc tccacgtttg gcggccggga tcgggtaatc
    18481 ctgataatcc agcagcgcgt caagtgcacg aaccgccaga attgccagct cttccagttc
    18541 atccaggtta ttaattgcgc ccaggttgaa agcagacagc gtacacagcg cgatttcacc
    18601 gttctcgtcg ttgacgtcgt tcagcggttt ggtcggcagg gctatctcca ggcacaggtt
    18661 agactgacgc actggcgcga tggccggatc aaacgggcta tgggtattgc agtggtcaac
    18721 gttctgaata tagatacgac cggtagacgc acgttcctgc atcatcagcg agaacagctc
    18781 aacggctttc acacgctgct tgcggatgct gtcgtctttc tcatatttgg tatacagacg
    18841 ttcaaactct tcctgatcgg cgaagaacgc gtcgtacagc cccggtacgt cggacgggct
    18901 gaacagggtg atatcttcac ctttcagcag acgggtatac atcagtttgt tgatttgtac
    18961 cccgtagtcc atatgacgca cgcggttgcc ttccacacca cggttgtttt tcaacaccag
    19021 caggctttcc acttccagat gccacatcgg gtagaacagc gttgccgcac cgccgcgcac
    19081 accgccctga gagcaggatt tcaccgctgt ctggaaatgt ttgtagaacg gaatgcagcc
    19141 ggtatggaac gcttcaccac cgcgaatcgg gctacccagc gcacgaatac gcccggcgtt
    19201 gatgccgatc ccggcacgct gggaaacgta tttaacaatc gcgctggagg tggcgttgat
    19261 ggaatccagg ctgtcaccgc actcgatcag tacgcaggag ctgaactgac gagtcggggt
    19321 acgcacgccg gacatgattg gcgtcggcag cgaaatttta aatgtggaaa ccgcgtcgta
    19381 aaaacgcttc acatattgca ggcgcgtttc acgcgggtag ttcgagaaca ggcacgcggc
    19441 aaccagaata taaaggaact gggcgctctc atagatttcg ccggtcacgc ggttctgtac
    19501 cagatatttg ccttccagct gcttaacggc agcataagag aaggtcatat cacggtcgtg
    19561 atcgataaac gtgtccatct gcttgaactc ttcttccgtg tagtcttcca gcagatgatt
    19621 atcgtatttg cccatctcga ccattttcac cacgtggtcg tacagcgcag gcggctcaaa
    19681 ctggccgtag gcttttttac gcaggtggaa gatcgccagg cgcgcggcga gatactgata
    19741 atccggcgca tcacgggaga tcaggtctgc ggcagccttg ataatggttt cgtggatgtc
    19801 agaggtcttg ataccgtcat aaaactgaat gtgggagcgc agctcgacct gggaaatcga
    19861 aacgttatgc agtccttctg ccgcccaatc cagaacgcga tggattttgt cgagattgat
    19921 gcgctctgtg ctaccgtcgc gctttgtcac cagcagattc tgattcatgt atgtcgtacc
    19981 tgtttttgga attctttccg caatagtttc atgcagacta cctgtagtaa gggaagctgc
    20041 ttagaacact atatataggg ggtatgtttg ggattcactg caagatagtg tgaaaatgac
    20101 cctcttgcaa gtgcataact ttgtggataa ctcaggaagg aaaaagtggc tttcgcgcac
    20161 cttaggtcag acaaggtgtc cgggaaagtc aatgggaaga gaaaaatttg ttaaaaataa
    20221 ctgttcggcg atttcttgaa cggcagaata ggttggataa attgatatag atggtttatt
    20281 aatctttttg ttaatgattg caaaaattga tctaagcacg agtcatcaca taatctgatg
    20341 tatcagttgt ggtcaatata taagcgaatg taagcgattg ttgatgtcga aatggttgtt
    20401 ttctattcgg ttattgttaa atgcgaagc
//
LOCUS       DAZPLI010000066        20427 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZPLI010000066 DAZPLI010000000
VERSION     DAZPLI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.067"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="ACHUU3_002294"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="ACHUU3_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR2082339.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="ACHUU3_002295"
     CDS             433..621
                     /locus_tag="ACHUU3_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR2082340.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="ACHUU3_002296"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="ACHUU3_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR2082341.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="ACHUU3_002297"
     CDS             816..989
                     /locus_tag="ACHUU3_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR2082342.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="ACHUU3_002298"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="ACHUU3_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHUU3_002299"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHUU3_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFR2082343.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFATQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="ACHUU3_002300"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="ACHUU3_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR2082344.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHUU3_002301"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHUU3_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR2082345.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="ACHUU3_002302"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="ACHUU3_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR2082346.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNESDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="ACHUU3_002303"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="ACHUU3_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10407..10952
                     /gene="torD"
                     /locus_tag="ACHUU3_002304"
     CDS             10407..10952
                     /gene="torD"
                     /locus_tag="ACHUU3_002304"
                     /inference="COORDINATES: protein motif:HMM:NF003442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR2082347.1"
                     /translation="MFSRELDDEQLTQISSAQMAEWFSLLKSEPPLTAAVNALENRIA
                     ALTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKRLLVEAGMETSGNFN
                     ESADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTALRQWLPEFAARCRQYDSF
                     GFYAALSQLLLVLVECDHQNR"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHUU3_002305"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHUU3_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR2082348.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHUU3_002306"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHUU3_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR2082349.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            12531..13677
                     /locus_tag="ACHUU3_002307"
                     /pseudo
     CDS             12531..13677
                     /locus_tag="ACHUU3_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13971..15212
                     /gene="agp"
                     /locus_tag="ACHUU3_002308"
     CDS             13971..15212
                     /gene="agp"
                     /locus_tag="ACHUU3_002308"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR2082350.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(15250..15477)
                     /locus_tag="ACHUU3_002309"
     CDS             complement(15250..15477)
                     /locus_tag="ACHUU3_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR2082351.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(15498..16094)
                     /gene="wrbA"
                     /locus_tag="ACHUU3_002310"
     CDS             complement(15498..16094)
                     /gene="wrbA"
                     /locus_tag="ACHUU3_002310"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR2082352.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            16467..16640
                     /locus_tag="ACHUU3_002311"
     CDS             16467..16640
                     /locus_tag="ACHUU3_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR2082353.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(16898..18226)
                     /gene="rutG"
                     /locus_tag="ACHUU3_002312"
     CDS             complement(16898..18226)
                     /gene="rutG"
                     /locus_tag="ACHUU3_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR2082354.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(18247..18741)
                     /gene="rutF"
                     /locus_tag="ACHUU3_002313"
     CDS             complement(18247..18741)
                     /gene="rutF"
                     /locus_tag="ACHUU3_002313"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR2082355.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(18752..19342)
                     /locus_tag="ACHUU3_002314"
     CDS             complement(18752..19342)
                     /locus_tag="ACHUU3_002314"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR2082356.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(19352..20152)
                     /gene="rutD"
                     /locus_tag="ACHUU3_002315"
     CDS             complement(19352..20152)
                     /gene="rutD"
                     /locus_tag="ACHUU3_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR2082357.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPC
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(20160..>20427)
                     /gene="rutC"
                     /locus_tag="ACHUU3_002316"
     CDS             complement(20160..>20427)
                     /gene="rutC"
                     /locus_tag="ACHUU3_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR2082358.1"
                     /translation="NVLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWK
                     NYAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         5106 a         5200 c         5243 g         4878 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggc gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcgag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gtggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgagtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatctc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtttaa ctagggcgtc attatttatt aaattttata gacgctatat atgggtagta
    12541 atatacatgg aattagttgc actgcaaata attatttgaa acaggcctgg aacgatataa
    12601 aaaatgagta cgaaaaaaat caaacatatt caatcacgct ttttgaaaac acactggtgt
    12661 gttttatgcg gttatacaat gaactcagac gtaaagtaaa tgaagaggat actccatgtc
    12721 tggaatgtga atcactagaa aaagaatttg aggaaatgca gaatgataat gatctatcat
    12781 tatttatgag aacattgcgt actaatgata cacaaattta ttcaggggtt tcaggaggta
    12841 ttacatatac tatacaatat gttcaaaatg ttgatattgt tagagtgtcc ttgccgggca
    12901 gaggttcaga gtctatcaca gattttaaag gttattattg gtatggcttt atggaatata
    12961 ttgaaaacat tactgcgtgt gatgatattt tttctgagta ttgtttagat gatgaaaata
    13021 tgagtatcca gccagaacag ataaatacgc cgggaatatc tgatttagat actggtttta
    13081 aaagaacatg agtattcatg ggcgctgggc ggccataaga cgcaaaagca agtatacgaa
    13141 gagtttatta aaattgttga taaacaaacg atcgaggctc ttgttgatac ggcattctat
    13201 aatctcaggg aagatgtaaa gacgttattt ggcgttaatc tacaatctga caacaaaatt
    13261 caggggcagg gtggtttttt atcatggagt tttttgtctt ttaaaaaaga atttattgat
    13321 agttgcttga atgacaaaaa atgcatactg catttacccg aatttatatt taatgataac
    13381 aaggcacgcc ttgttttaga caccgaccca gagcagaagg ttaatgaggt gaaaaatttc
    13441 ctcactgctc tttcagatag catttgctca ttatttattg tttacagcaa tgtggcatca
    13501 atctccttag ggaatgaatc cttttcaaca gatgacgacc ttgagtatgg gtatttaata
    13561 aacactggca atcattatga cgtttacctc cctcctgaac tttttgctca ggcttacgag
    13621 ttaaacaata aggaaaggaa tgcgcaaatt gattttttaa ctcgttatgc aatttaatga
    13681 caaaggcatg tgctaaaaac cattgttatt agcctcactt ctcttattat tggtactatt
    13741 gcctctgtat tagtaacgcc gctgatattc tgtttagcca caggtgcaat tatcagcggc
    13801 gtacgcgagg caggggctaa tcaggcatag tttgcgtcaa accttgcctg tttttgaaga
    13861 tatatataga aaaacaggcg ttcaacaagc cattttgcga acctgttccc ggaaaaaagt
    13921 catatttctg tcacactctt taatgattga taacaaaaga ggtgccagga atgaacaaaa
    13981 cgctaatcgc cgcaactgtg gcagggatag ttttactcgc ttcaaatgct caggcacaaa
    14041 ccgtaccgga aggctatcag ctacagcaag tgctcatgat gagccgccat aacttacgtg
    14101 cgccgctggc gaacaatggc agtgtgctgg agcagtcgac gccgaataaa tggccagaat
    14161 gggacgtccc cggtgggcaa ctcaccacca aaggtggcgt gctcgaagtg tatatgggcc
    14221 attacatgcg tgaatggctg gcagagcagg ggatggtgaa atcgggggaa tgcccgccgc
    14281 cggacaccgt ttatgcctat gccaatagtc tgcaacgtac cgttgcgacc gcacagttct
    14341 ttattaccgg cgcattcccg gggtgtgata ttcctgtgca tcaccaggaa aaaatgggca
    14401 ctatggaccc aacctttaac ccggtgatca ccgatgattc cgccgcattc agtgaacagg
    14461 cggtggcggc aatggagaaa gagctcagca aactccagct taccgacagc taccagctac
    14521 tggaaaaaat cgttaactat aaagattccc ctgcctgtaa agagaaacaa cagtgttcgc
    14581 tggtggatgg caaaaatacc tttagcgcca agtatcaaca agaaccaggt gtttccgggc
    14641 cgctgaaagt cggcaactcg ctggtagatg cgtttacttt gcaatattac gaaggttttc
    14701 cgatggatca ggtggcctgg ggagaaatca aatctgacca gcagtggaag gtgttgtcga
    14761 agcttaaaaa cggctaccag gacagcctgt ttacctcacc ggaagtggcg cgcaatgttg
    14821 cgaaaccgct ggtcagttat atcgacaaag ctctggtcac cgatcgcacc agcgcaccga
    14881 aaattacagt gttggttggg cacgactcca acattgcctc tctgttaacg gcgctggatt
    14941 tcaaaccgta tcagttgcat gaccagaacg aacgcacgcc gattggcggc aaaatcgttt
    15001 tccagcgttg gcatgacagc aaagccaatc gcgatttgat gaaaattgaa tatgtgtatc
    15061 agagtgcgga acagttacgt aatgccgatg cgttaaccct gcaggcacct gctcagcgtg
    15121 tgacgctgga attaagcggt tgcccgatag atgccaacgg tttctgcccg atggataaat
    15181 ttgatagcgt gttgaatgaa gcggtgaaat aacagaaaac tcccccgcga gaagcggggg
    15241 agtcgctggt taaacgtttt tacgttcgat ggtctgttcg ccccaaaaaa gcgaatcttt
    15301 atcggcctta gcaaaggctt tgactaacac ttcatcgcta ccttcttccc aaatcttttc
    15361 tgccattttt tcgtcgtacc cggcgacttc gaaaatggcc tcggctattt ccggcgacgt
    15421 attgcgcaga gatgcccatt caccgacgtg atgagctttc gcttcttgag ttggcatgcg
    15481 tatcctcctg ttgaagatta gccgttaagt ttaactgcca gacctgcgac atattcccct
    15541 tgataacgag caatagacag ttcttcctgg cttggctggc gtgagccgtc accgcctgcg
    15601 atggtggttg cgccgtacgg cgtaccgccg cgaacctgtg aaacgtcaaa taattcctgc
    15661 gctgcgtagc caatggggac aatcaccatg ccgtgatgcg caagggtcgt ccaggttgaa
    15721 gtaatagttt gttcctgacc gccgccagta ccggtggaac taaagacgct cgccagtttt
    15781 ccgtatagtg cgccggaagc ccacaggccg cccgtctggt cgaggaaggt acgcatttga
    15841 ccggacatgt tgccaaagcg ggtaggtgta ccaaaaataa tggcgtcgta atcggccagt
    15901 tcttgcgggg ttgcaaccgg tgcagtttgc gttttaccgc ctgctttttc aaataattgc
    15961 ggcggcatgg tttccggtac acgcttaacg acaacttctg cgccatcgac tttgcttgca
    16021 ccctcagcga ctgcgcgtgc catcgtttca atatgtccgt acatggaata ataaagcacc
    16081 agaactttag ccatttctaa ccactcctcg tgttatctct attccgtagc gattcgctac
    16141 cacttattta aagataagac gtccttttca gagtgcaaat ttcacaacca cttatttgat
    16201 ttataacaac tttcacaagc acgtaatttt gtcgcaaaat gacacatttt tatctcatcg
    16261 cgttttttta atcataagag cggcttatgg ataattattg gagatgatat ctattctcgc
    16321 taagaagctg ttgcaggata ttaccaaacg cgggtctacc cgcgtcagtt cactaagctt
    16381 agtcccacgt agcgaaaata tggcagccgc catacgccgc gttaattcta tgcaatatga
    16441 tgtctatacc cagacggagg tcagtaatgg caaaccatcg aggcggttcc ggcaattttg
    16501 cagaagaccg cgaaagagca tcagaagcag gtaaaaaagg tggacagcac agcgggggta
    16561 atttcaagaa tgacccgcag cgcgcatctg aagcaggtaa aaaaggtggc aagagcagtc
    16621 acggcaaaag cgacaactag ccgggctaat caatgacgaa tgcatttttg tctgtagctc
    16681 gtcaaaaagc catcaccgcc ggttacccgg tggttgatac tgatgacaaa tgtaagcttg
    16741 cctgatgcgc gatgcttatc aggcctacca gaagattgca atatattgaa tttgcactgt
    16801 tttgtaggcc ggataaggcg tttacgccgc atccggcatg aacaatgcgt acgttgtcaa
    16861 caatctgcac cgccgggtaa ccccggcggt tttctgttta tggctcctga tgaacaactt
    16921 ctggcggtgg aacgtcaacc aaccttctac ttaacaacgc attgagtaaa atcgcgccaa
    16981 aggtggctgt accgatccct cccaacgtaa aaccgcccag cgtgagagca aaatcacccg
    17041 cgcccagcac taaggttact gcgaccataa tcaaattacc gttctggctt aaatcgacac
    17101 ggttttgtac ccagatcctt gcgcctgcga cggcaatcag cccgaacaca acaattgatg
    17161 caccaccaat caccgcggcc ggaatggtat ggatcagcgc gccaaatttc ggcgaaaagc
    17221 ccaacagcat ggcgatgacg gcagcagcaa caaacaccag cgtcgagtag acttttgtca
    17281 cggccatcac accgatattt tcagcatagg tggtgacgcc gctaccgccg acagagccgg
    17341 aaagcatcgt tgccagacca tcaccgacga atgctcgccc catatacggg tccatattgc
    17401 gtccggtcat cccggcgacc gccttgagat gacctaagtt ttccgccacc agaatcaccg
    17461 ccacgggcgc aatcagcatc attgcctgac tattaaaagc aggagtggaa aaatgtggca
    17521 aaccgaacca ggcagcatgg ctgacgagag taaaatcgac ggcttttccc agccctaaaa
    17581 cgttggtcat cacgccatac agcagacagg cgacaattaa acctacgaga atcaataacc
    17641 gctggatcat gccacgggta aacaccgcca ccagcccaat acacagcacc gtcattaccg
    17701 ccatccagct atcaaaggcc gaagccgata cacttttcac tgcgataggc gctaagttca
    17761 ggccaatcgc catcaccacc gcacccgtca ccaccggcgg cattagtcgt tcaatccagc
    17821 gtgtaccaat tttcatcacc accaggccaa tgacggtata aaccagccca caggcgataa
    17881 tcccgcccag tgcgatgctg atattcgggt taatgccctg accgttaaag cccgtcgcgg
    17941 caatcaccac gccgacaaaa gccgcgctgg agccgagata actggggacg cgcccgccgg
    18001 taataaagaa aaacagtaac gtgccgatcc ccgacattaa aatggaaaga ttgggatcca
    18061 gccccatcag aatcggcatt aacaccgtcg cgccaaacat cgccaccgcg tgttgaacgc
    18121 ccattactgc cgtctgagca aacggcaatc gttcatccgg cgcgaccacg ccgctctctg
    18181 tagaggtcga ttttaactgc cagtgaggaa aaccgaacat tgccatcagc tgtctcctta
    18241 aggaggttaa caagcagggc gcatcagcgc gtgataactg cgatcgaacc acaccagccc
    18301 gtagggtgtg gtgtgacgat gaatcgcttc gatggcgcaa aacagaatgt cgtgggtgcc
    18361 gacgctcacc acctggctga tgcggcagtc aaacgaaacc agagcctctt ccagttgcgg
    18421 gcatccggtc acacccgtct gccagcgggc ggcggcaaag cggtgttcca tgggcgtttt
    18481 gccgccaaaa agatttgaaa gcggctcctg cccggcgctc agtgtattta cacacagcgt
    18541 tcgattttca ttgaataccg gccagacgga cgccccacga ttcaggcaca ccagtaatgt
    18601 gggcggcgtg tcggtcacgc tgcagacggc gctggcggtg aacccggcgc gcccggatgg
    18661 accgtccgtg gtgatgatat tgaccgccgc gcccatacag gacatcgcat cgcgaaaggt
    18721 ttgttgatca actatgttca tagtttgctc cttacaacag cccgcaggct tcttcaaagg
    18781 acagacgtgg caggcgcgca aaaagtttgc tgctatcgcc atagccgata ttaatcagca
    18841 gattgctctt cagcgtgctg cccgcaaaaa aggcgtcgtc aacgtgttga cggtcaaaac
    18901 ccgacatcgg gccggtatcc agtcccagcg cacggcaagc gacgatcaga taggccgcct
    18961 gcatggagct gttgcgaaac gctgtttctt cggcaagttg tgggctggag gtaaaccaac
    19021 tgcgggcatc accgtgggga aacagtagtg gtaaccgttc ataaaattca ctgtcccagg
    19081 caacgatagc ggtgacgggc gcggtcaggg ttttttgtag attgccgctg gaaagcgccg
    19141 ggtgcagacg ttcttttcct tctacagtgc ggataaacac gatccgtgcc ggagaacagt
    19201 tagctgatgt cggcccccat tttatcgggg cataaagctc ccgtaacgtc tcatcgctga
    19261 cgggtgtctc ccgccagccg ttgtgagtgc gggcatcggt gaacagggtg ctaagcgcac
    19321 ctgggctaac ggcttcgttc atagcaattc cttacagggc ggcttcacgg tgatgtaaca
    19381 ggctggcaag cccgttgagt aacagagcat taaacgtttc ggggtcggtc acgttgcagg
    19441 cgtgtccgcc atagcgcatc accattttct ggctatcggg cagggcggca tgaagctcac
    19501 tggaacatgc tgatggcacc agcagatcat cactggcgca gatgatttgc accgggcagc
    19561 ggatgcgatc cgcatggtga ctaaagtcag cgcgtttgag ggcgttaagt cgacgcagta
    19621 aattattttt gccctgaaaa tgcgccagtg ccagcgcgtc ttctgcctca aggcaaggtg
    19681 cgcgggccgc catccagtcg gcgggataga ggaacaacgg ctgcgcttcc acccatgcct
    19741 gcgcgccgcc gctatacagt aatcgttcgc gaacctgaaa acagcggcgc gtatgggcgt
    19801 ttattcgtag ccagccgtta acgctgatca gcacagttac cgacgcggga taatccagcg
    19861 ccagctgcat tcccaccagc gcaccgagcg catggccgac caccgcgtaa tgctcaatcc
    19921 ctgcggctac cagcgcctga tgcagttccg ctgccatctg ggcgatactg taatcttctg
    19981 ccagcgtgtc gggattattg ccggtgccgc gctggtcgta acagactacc tgatactcct
    20041 gctccagcac cgccagttgc ggtaaccagt aactgccgct accgccaaga cccgaaatca
    20101 acaccactac gggcgcatca gcataagggg gaggtgagag tgaaagtttc atcgcggcct
    20161 cacttggcga tatgcgcaat tgtggcgatt tccaccagcg cgtcaggttt taccagtccg
    20221 cactgaatgc agaatcgcgc cggtttatca cccggaaaaa actcggcgta gatttcgtta
    20281 atcgcggcgt aatttttcca gtcggtaata aagatgctgt tgaaggtcac atccgccatc
    20341 gtgccacccg ccgtctcgat caccttgcgg atagtttcca gaacgtggcg ggtttgcgcc
    20401 tttgggtcat cggcaaacag cacgtta
//
LOCUS       DAZPLI010000067        20259 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZPLI010000067 DAZPLI010000000
VERSION     DAZPLI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20259
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.068"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(230..1879)
                     /gene="pgi"
                     /locus_tag="ACHUU3_002317"
     CDS             complement(230..1879)
                     /gene="pgi"
                     /locus_tag="ACHUU3_002317"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR2082359.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2116..2312
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2404..3753
                     /gene="lysC"
                     /locus_tag="ACHUU3_002318"
     CDS             2404..3753
                     /gene="lysC"
                     /locus_tag="ACHUU3_002318"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR2082360.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            4005..4277
                     /locus_tag="ACHUU3_002319"
     CDS             4005..4277
                     /locus_tag="ACHUU3_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR2082361.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4410..5282)
                     /gene="rluF"
                     /locus_tag="ACHUU3_002320"
     CDS             complement(4410..5282)
                     /gene="rluF"
                     /locus_tag="ACHUU3_002320"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR2082362.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDDERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5630..6467
                     /locus_tag="ACHUU3_002321"
                     /pseudo
     CDS             5630..6467
                     /locus_tag="ACHUU3_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            6537..7343
                     /locus_tag="ACHUU3_002322"
     CDS             6537..7343
                     /locus_tag="ACHUU3_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR2082363.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7353..7760
                     /locus_tag="ACHUU3_002323"
     CDS             7353..7760
                     /locus_tag="ACHUU3_002323"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR2082364.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            7760..8254
                     /locus_tag="ACHUU3_002324"
                     /pseudo
     CDS             7760..8254
                     /locus_tag="ACHUU3_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            8320..9117
                     /locus_tag="ACHUU3_002325"
     CDS             8320..9117
                     /locus_tag="ACHUU3_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR2082365.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            9128..9952
                     /locus_tag="ACHUU3_002326"
     CDS             9128..9952
                     /locus_tag="ACHUU3_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFR2082366.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            10004..11232
                     /gene="sorE"
                     /locus_tag="ACHUU3_002327"
                     /pseudo
     CDS             10004..11232
                     /gene="sorE"
                     /locus_tag="ACHUU3_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            11615..12304
                     /gene="pepE"
                     /locus_tag="ACHUU3_002328"
     CDS             11615..12304
                     /gene="pepE"
                     /locus_tag="ACHUU3_002328"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFR2082367.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12395..14026)
                     /locus_tag="ACHUU3_002329"
     CDS             complement(12395..14026)
                     /locus_tag="ACHUU3_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR2082368.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14246..17929)
                     /gene="metH"
                     /locus_tag="ACHUU3_002330"
     CDS             complement(14246..17929)
                     /gene="metH"
                     /locus_tag="ACHUU3_002330"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR2082369.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18129..18953
                     /gene="iclR"
                     /locus_tag="ACHUU3_002331"
     CDS             18129..18953
                     /gene="iclR"
                     /locus_tag="ACHUU3_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR2082370.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            19270..20259
                     /gene="espL4"
                     /locus_tag="ACHUU3_002332"
                     /pseudo
     CDS             19270..20259
                     /gene="espL4"
                     /locus_tag="ACHUU3_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT         4906 a         5211 c         5204 g         4938 t
ORIGIN      
        1 acgattaaaa atctatttta ggattaacct cacggtatga tttccgttaa atcacagaca
       61 aggggattta tctgataaaa caatgccgac ttattgtcgg cattgttttt aaggttattt
      121 aactcctcat actatgcatt aatgtcgata ttggcatcag gctccggttg ccggatgcgg
      181 cgtaaacgcc ttatccggcc tacatatcgg catcaggcac gcatgatgat taaccgcgcc
      241 acgctttata gcggttaatc agaccattgg tcgagctatc gtggctgctg atttctttgt
      301 catctttcag ctctggcaga atacggttcg ccagttgttt acccaactcc acgccccact
      361 ggtcgaaggt aaagatgttc aggatcacgc cctgagtaaa gattttgtgc tcatacagcg
      421 caatcaacgc acccaggctg aacggagtga tttcgcgcag caggatggag ttggtcgggc
      481 ggttaccttc gaacactttg aacggcacca cgtagtcaag cgttgccgga tctttaccct
      541 gatcgcgata ttcctgctca accacttcgc gggatttacc aaacgccagc gcttcggtct
      601 gggcgaagaa gttagacagc agtttctggt ggtgatcaga gagcgggtta tgggtgatag
      661 ccggggcgat gaaatcgcac ggtaccattt tggttccctg gtggatcagc tggtagaacg
      721 cgtgctgacc gttagtgcct ggttcacccc agataatcgg gccagtctgg taatccacaa
      781 cgttaccgtt acggtcaaca tacttaccgt tggactccat attgccctgc tggaagtacg
      841 ccgcgaaacg gtgcatatac tggtcatacg gcagaatcgc ttcagtttcc gcaccaaaga
      901 aattgttgta ccagatgcca atcagcgcca gcaggacagg caggtttttc tcggcaggcg
      961 tggtggagaa atgcttgtcc atcgcgtgtg cgccagaaag cagttcaacg aagttatcaa
     1021 agccgatgga gagaacaatc gacaggccaa tcgctgacca caaagagtaa cggccgccga
     1081 cccagtccca gaactcgaac atgttggcag tatcaatacc aaactcgcca acggctttgg
     1141 cattggtgga aagcgccgca aagtgttttg caacgtgttt ttcatcacct gccgctttca
     1201 ggaaccagtc acgcgcgcta tgggcgttgg tcatagtttc ctgagtggtg aaggttttag
     1261 atgctaccag gaacagcgtg gtttccgggt ttactttttt cagcacttca gcgatgtgag
     1321 tcccatcgac gttagaaaca aagtgcatgt tcaggtgatt tttgtacgga cgcagtgctt
     1381 cggtcaccat gtacgggccg aggtcagaac caccgatccc gatgttcact acgtcagtga
     1441 ttgctttgcc ggtataaccc ttccactcac cggagataat cgcttccgag aaggttttca
     1501 tcttctccag caccgcgttg acttccggca ttacgtcttt gccatcaacc agaatcgggg
     1561 tattgctacg gttacgcagc gctacgtgca gcacggcgcg gttttcagtg cggttgatct
     1621 tctcgccaga gaacatcgac ttaatcgcgc ccgccagatc gcactctttc gccagatcct
     1681 gtaatttcgc cagcgtctct tcagtgatgc ggtttttgga gtaatccacc agcatctgat
     1741 cgtcgaatgt tgcggagaac ttagaaaaac gatcgccgtc tttagcaaaa agatcggcga
     1801 tcgtaacgtc tttcatttca tcgaagtgtt tctgtagtgc ctgccaggca gcggtctgcg
     1861 ttggattgat gtttttcatt agcaatactc ttctgatttt gagaattgtg actttggaag
     1921 attgtagcgc cagtcacaga aaaatgtgat ggttttagtg ccgttagcgt aatgttgagt
     1981 gtaaaccctt agcgcagtga agcatttatt agctgaacta ctgaccgcca ggagtggatg
     2041 aaaaatccgc atgaccccgt cgttgacaac cgccccgctc accctttatt tataaatgta
     2101 ctacctgcgc tagcgcaggc cagaagaggc gcgttgccca agtaacggtg ttggaggagc
     2161 cagtcctgtg ataacacctg agggggtgca tcgccgaggt gattgaacgg ctggccacgt
     2221 tcatcatcgg ctacaggggc tgaatcccct gggttgtcac cagaagcgtt cgcagtcggg
     2281 cgtttcgcaa gtggtggagc acttctgggt gaaaatagta gcgaagtatc gctctgcgcc
     2341 cacccgtctt ccgctcttcc cttgtgccaa ggctgaaaat ggatcccctg acacgaggta
     2401 gttatgtctg aaattgttgt ctccaaattt ggcggtacca gcgtagctga ttttgacgcc
     2461 atgaaccgca gcgctgatat tgtgctttct gatgccaacg tgcgtttagt agtcctctcg
     2521 gcttctgctg gtatcactaa tctgctggtc gctttagcgg aagggctgga acctggcgag
     2581 cgattcgaaa aactcgacgc tattcgcaac atccagtttg ccattctgga acgtctgcgt
     2641 tacccgaacg ttatccgtga agagattgaa cgtctgctgg agaacattac tgttctggca
     2701 gaagcggcgg cgctggcaac gtctccggcg ctgacagatg agctggtcag ccacggcgag
     2761 ctgatgtcga ccctgctgtt tgtcgaaatc ctgcgcgaac gcgatgttca ggcacagtgg
     2821 tttgatgtac gtaaagtgat gcgtaccaac gaccgatttg gtcgtgcaga gccagatgta
     2881 gccgcgctgg cggaactggc cgcgctgcag ctgctcccac gcctcaatga aggcttagtg
     2941 atcacccagg gatttatcgg tagcgaaaat aaaggtcgta caacgacgct tggccgtgga
     3001 ggcagcgatt atacggcagc cttgctggcg gaggctttac acgcatctcg tgttgatatc
     3061 tggaccgacg tcccgggcat ctacaccacc gatccacgcg tagtttccgc agcaaaacgc
     3121 attgatgaaa tcgcgtttgc cgaagcggca gagatggcaa cttttggtgc aaaagtactg
     3181 catccggcaa cgttgctacc cgcagtacgc agcgatatcc cggtctttgt cggctccagc
     3241 aaagacccac gcgcaggtgg tacgctggtg tgcaataaaa ctgaaaatcc gccgctgttc
     3301 cgcgcgctgg cgcttcgtcg caatcagact ctgctcactt tgcacagcct gaatatgctg
     3361 cattctcgcg gtttcctcgc ggaagttttc ggcatcctcg cgcggcataa tatttcggta
     3421 gacttaatca ccacgtcaga agtgagcgtg gcattaaccc ttgataccac cggttcaacc
     3481 tccactggcg atacgttgct gacgcaatct ctgctgatgg agctttccgc actgtgccgg
     3541 gtggaagtag aagaaggtct ggcgctggtc gcgttgattg gcaatgacct gtcaaaagcc
     3601 tgcggcgttg gcaaagaggt attcggcgta ctggaaccgt tcaacattcg catgatttgt
     3661 tacggcgcat ccagccataa cctgtgcttc ctggtgcccg gcgaagatgc cgagcaggtg
     3721 gtgcaaaaac tgcatagtaa tttgtttgag taaatactgt atggcctgga agctatattt
     3781 cgggccgtat tgattttctt gtcactatgc ttatcaataa acgagcctgt actctgttaa
     3841 ccaacgtctt tatcgagaat aattgccttt aattttttta tctgcatctc taattaatta
     3901 tcgaaagaga taaatagtta agagaaggca aaatgaatat tatcagttct gctcgcaaac
     3961 gaattcccgt acactgaact ctctggctca ctgagggcac catcatggca ctcccccgta
     4021 ttacccaaaa agagatgact gaacgcgagc agcgtgaact taaaacactg ctggatcggg
     4081 cgcggattgc gcatggtcgc gtactgacca actccgaaac caacagcatt aagaaagagt
     4141 acatcgataa attaatggtc gagcgtgagg ctgaagcgaa aaaagcccgc caattgaaga
     4201 aaaagcaggc ttataaaccg gatcctgaag cgtcatttag ctggtcagca aatacgtcga
     4261 cgcgcggcag gcgctaatga acaatcaacg cctgagattt tcactcgtca gtgtgaacac
     4321 aattttcccc tcaccctaac ccttttcccg aaggggcgag gggactgtcc gagcactttt
     4381 gggccgtttt catcagtctg aggcgttaat cagcgcccct tctttttacg ccccggcgag
     4441 gtaaaacgct taccgttgga cgccgggcga ccgccttttt ccgccgtttt ttccatctta
     4501 acgactggac gtttgatgcc cgctgttttc ggtttggcct tcgctttggg cttcgcctcg
     4561 gaagaggaat tttcaatgag cttaaagaga tcgataagct cgtcgtcggt taaatcgcgc
     4621 cattctccca gcggaatgcc gcttaagctg acgttcataa tgcgcgtacg ttccagcttt
     4681 ttcacttcat agccgaaatg ctcgcacatg cgccggatct gacggttcaa cccctgcacc
     4741 agggtaatgc ggaagacaaa cggtgcttct tttttgactt tgcacttttt cgtcaccgtc
     4801 ccgaggatcg gcactcccgc ccccatacca cgaataaatt catcggtaat cggtttatcg
     4861 accgtcacca gatactcttt ctcatgatcg ttacccgcac ggaggatctt attcaccaga
     4921 tcgccgtgat tggtgaggaa aatcagcccc tgggagtctt tatccaggcg gccaatcggg
     4981 aacacgcgtt tgctgtggtt aacgaaatcg acaatgttat cgcgctcgtc atcttcggtg
     5041 gtgctgacaa taccgacggg cttgttcagg gcgataagta ccaaatcttc ggcttcccga
     5101 ggctcaatca actgaccgtt tactttcaca atgtcgccgg gtttcacctg atcaccaatg
     5161 gtggctcgct tgccattaag gaacacattg ccttgctcga tatagcgatc cgcttcgcgg
     5221 cgtgagcaaa ttccgctttc gctgatgtat ttatttaaac ggactgatga gtcgggcagc
     5281 ataaattctc ctgtaaaacg cggaatatac cgtacctctt agcggggaaa aacagggtta
     5341 tgcggcaacg tcgaaattaa atgtgatctg ccacgccttt tatcacaaaa gtgtctccgg
     5401 aagtacgatg caaatttgcc tggcaaaata aggataatca acatattacg aatcgctgca
     5461 cacctgtgct gatgactact acgcagagaa acaaatgtgc aagagaaagc gctatttctc
     5521 cccatgcaaa gagtgaaaaa tcgctttgaa aggccctcat aatggggtta tatcagatca
     5581 agcggtgaga aagcgtattt gcacaaatgt gcagaaccag gaggcggcaa tggaaaacag
     5641 tgacgatatc cgtttgattg tgaagattgc ccaactctat tacgaacagg atatgacgca
     5701 ggcgcaaatc gcgcgcgaac tgggtattta ccgcaccacc atcagccgct tgcttaaacg
     5761 aggccgcgat cagggaattg tcaccatcgc catcaactat gactacaacg aaaatctctg
     5821 gctggagcag caactgaagc aaaagtttgg cttgaaagac gttgtggtgg tgtcgggaaa
     5881 tgatgaggat gaagagactc aactggcgat gatggggtta cacggcgcgc aactgctgga
     5941 tcgcttgctg gagcctggcg atattgtcgg tttttcctag ggtcgcgcgg tgagcgcact
     6001 ggttgaaaac ttgccgcaag cggggcaatc gcggcagtta atctgcgtgc caattattgg
     6061 cggaccgtcc ggtaaactcg aaagccgcta tcacgtaaac acattaacct acagcgtggc
     6121 agcgaagctg aaaggggaat cgcatctcgc ggattttccg gctctgctgg ataacccctt
     6181 aattcgtaat gggatcatgc agtctcagca ctttaaaacc atctctgcct actgggataa
     6241 tctggatgtc gccctggtgg gaattggctc accggccatt cgcgacggcg ctaactggca
     6301 tgcgttttat ggtggtgaag agagtgacga cctgaaagca ggcgcggtat tccattggca
     6361 ttgccatgag tgaagaaaaa tacagcggaa ttgttggtgc actgcgtgga aaatatatta
     6421 attgtctggt aacgaatagc agcacagctg aactattact gaaataaaaa tcttcccgat
     6481 gtttaccgta ataaaaatta cggcgggtcc ctattatcta aaatcaggag tctgttatgc
     6541 aaacgtggtt aaatttgcag gataaaatca ttatcgtcac cggcggcgca tccggtattg
     6601 gtctggcgat tgtagaggaa ttattagcac aaggcgcaaa cgtacagatg gtagatattc
     6661 acggtggcga tggtcaatat gaagttcata aaggctatca gttttggccg accgatattt
     6721 ccagcgccaa agaggtaaat catacggtag cggaaattat ccagcgtttt ggtcgcatcg
     6781 acggtctggt caataacgcc ggggtcaatt tcccgcgtct gctggtcgat gagaaagcgc
     6841 ctgccgggca gtatgaactc aacgaagctg cattcgaaaa aatggtcaat atcaaccaaa
     6901 aaggcgtttt tctgatgtcg caggcggtgg cgcgccagat ggtcaaacaa catgatggcg
     6961 tgattgtgaa tgtttcctca gaaagtggac tggaaggctc agaaggccaa agctgttacg
     7021 ccgctaccaa agccgcgctc aatagcttca cgcgctcctg gtcgaaagag ctgggtaaac
     7081 acggtatccg tgtggtcggt atcgcgccgg ggattctgga aaaaaccggg ctacgcacgc
     7141 cggaatatga agaggcgctg gcgtggacgc gcaatatcac cgtcgagcag ctgcgtgaag
     7201 gctataccaa aaacgccatt cctattgggc gcgccggaag attagcagaa gtcgctgatt
     7261 ttgtttgtta tctgctgtct gaacgcgcca gctacatcac cggagtaacc actaacattg
     7321 cgggcggcaa aacgcgcgga taaggaggct ttatggtcaa cgccattttt tgtgcccacg
     7381 gcaaattagc ttgcgccatg ctggaatcgg ttcagatggt gtacggcgac gccaatgtcg
     7441 aggcggttga atttgtacca ggcgagaacg ccggagacat cgtagcaaag ctggaaaagt
     7501 tagtaagcat tcacaatcaa gatgagtggc tgattgccgt ggatttacag tgcggcagcc
     7561 cgtggaatgc ggcagcgatg cttgcgatgc gcaatcctcg tctgcgggta atcagcggcc
     7621 tttccctgcc gctggcgctc gaactggttg ataaccagga cagcatgaat gttgatgaac
     7681 tgtgtgaaca cctgacgcaa atcgcaaagc aaacctgcgt tgtctggaaa caactggcaa
     7741 cggcagagga agatttctga tgaacattac gctcgcccgc attgatgacc gtctgatcca
     7801 cggccaggtc actaccgtct ggtcgaaagt ggctaacgcc cagcgcatta ttatctgcaa
     7861 tgacgaagtt tataacgatg aagtgcgccg gacattattg cgtcaggctg cgccaccggg
     7921 aatgaaggtt aacgtggtca atattgaaaa agccgtcgcg gtttatcaca acccgcaata
     7981 ttaggacgaa accgtttttt atcttttcac tcgcccacag gatgccttag cgatggttcg
     8041 ccagggggta aaaataggca ccctgaatat tggcggcatg gcctggcggc ccggtaaaaa
     8101 acagttaacc aaagcggttt cattagatga tgatgatatt aacgcatttc atgagttaaa
     8161 taatcttggc gtcataatgg atttacgcgt cgtagcgtca gatccctcaa tcaatattat
     8221 cgacaaaata aacgaacagt taatcgcaaa ttaaataata gcgcctgtgt acatatgctt
     8281 taacaggcag ggatgacgac acttaaaagg tgacaaatca tggaaataag taccctacaa
     8341 attattgcta tatttctttt ttcctgtatt gccggaatgg gcagcgtgct ggatgaattt
     8401 cagactcatc gcccgttaat tgcctgtacg gtgattggtt taattctcgg tgatttaaaa
     8461 accggaataa tactcggtgg tacgctggaa ttgatagctc tcggctggat gaacgtcggc
     8521 gcggcgcaat ctccggattc tgcactcgcc agcataatct ccgccattct ggttatcgtt
     8581 ggtcagcaaa gcatcgccac cggaatcgcc atcgcgttgc ctgtggctgc ggcaggccaa
     8641 gtgctgacgg tgtttgcccg taccatcacc gtagtgttcc agcacgcggc ggataaagca
     8701 gcggaagagg cgcggtttcg cactctcgat attctgcatg tctccgcgct tggcgtgcag
     8761 gcgctgcgcg ttgctattcc ggcactgatt gtctcactgt tcgtcagcgc cgatatggtc
     8821 agcaatatgt taaacgccat tcccgaattt gtgacccgtg gactgcaaat tgctggcggt
     8881 tttatcgtgg tggtcggtta cgccatggtg ctacgtatga tgggcgtgaa atatttgatg
     8941 cctttctttt tcctcggttt cctcgcaggt ggctacctcg atctcagcct gctggcgttc
     9001 ggtggcgtcg gcgtgatcat ggccctggtc tacatccagc taaatccaca gtggcgtaaa
     9061 gctgaacctc aactccaggc caccgcctcc accgcccttg accaacttga cgattaacgg
     9121 agccatcatg gaacagagaa aaattacacg cagcgatctg gtgagcatgt ttctgcgctc
     9181 caacctgcaa caggcgtcct ttaactttga acgtattcac ggactgggct tttgctacga
     9241 catgatcccc gccatcaagc gactttaccc attaaaagag gatcaggttg cggcgctcag
     9301 gcgacacctg gtgttcttca ataccacgcc agccgtatgt ggcccggtca tcggcgtcac
     9361 tgccgccatg gaagaggcgc gggccaacgg cgcggaaatt gatgacggta ccattaacgg
     9421 catcaaagtc ggtctgatgg gaccattggc aggagttggc gatccactgg tctggggaac
     9481 gctgcgcccg attaccgctg cgctcggcgc atctctggca ctttctggca acatcctcgg
     9541 cccgctgctg tttttcttta ttttcaatgc ggtgcgtctg gcgatgaagt ggtatggcct
     9601 acagctcggc tttcgcaaag gggtgaatat cgtcagcgat atgggcggga atgtgctgca
     9661 aaaactcacc gaaggcgcgt cgattctcgg actgtttgtg atgggcgtgc tggtcaccaa
     9721 atggacgtca atcaacgtac cgttggtggt ttcacaaacg catgccgccg atggctccac
     9781 cgtcatcatg accgtgcaga acattctcga ccaactttgc cctggtttgc tggcgctcgg
     9841 tctgacgcta ctgatggttc gtctgctcaa caaaaaaatt aacccggtat ggctgatttt
     9901 cgccctgttt ggcttaggga ttatcggcaa tgcgctgggc ttcctgtcct gattcttcgc
     9961 cccggcacga ctgccggggc catcgctcaa catgaggtgg tttatgaaaa caacagctct
    10021 gcgtctttat ggtaaacgtg atttacgcct ggaaaccttt gaccttcctg aaatgcagga
    10081 ggatgaaatc ctcgcgacgg tggtcactga cagcctgtgc ctctcttcct ggaaagaggc
    10141 aacctgggtg aaaaccataa aaaagtaccc gacgatgtgg cgaccaaccc aatcatcatc
    10201 ggccacgagt tttgcggcga tattctggcc gtgggtaaaa agtggcagca caaattccag
    10261 ccgggtcagc gttatgtgat tcaggccaac ctgaaactcc ctgaccgccc ggactgcccc
    10321 ggctactcct tcccgtgggt gggcggcgaa gccacgcatg tggttattcc caacgaggtc
    10381 atggaacaag attgcctgct ggcatacgac ggcgaaacct attttgaagg ctcgctggtt
    10441 gaaccgcttt cctgcgtgat tggcgcgttc aacgccaact atcatcttca ggaaggtagt
    10501 tataaccaca cgatggggat tcgcccgcaa gggcgcacgc tgatcctcgg cggcaccgga
    10561 ccaatgggat tgttggcgat tgattatgcg ctgcatggac cagttaaccc gtcgctgctc
    10621 gtcattaccg ataccgacaa cgacaaattg agttatgcgc gcaagcacta tccatcagaa
    10681 ccgcaaacac tgattcatta tctcaatgcc accgatgcag catttgatac gttaatggcg
    10741 ctgagtggcg gtcacggctt cgatgatatt ttcgtctttg tgcctaatga aggactggtg
    10801 actctcgcct cttccttgct ggcgacagat ggttgcctga atttcttcgc cggaccgcag
    10861 gataaacatt tcagcgcgcc aattaatttc tacgatgtgc attatgcatt tacccactac
    10921 gtgggcacgt caggcggcaa taccgacgac atgcgcgcgg cggtcaaact gattgaagag
    10981 aaaaaagtgc aggccgcaaa agtggtaaca catattcttg ggctgaatgc cgcgggcgaa
    11041 accacgcttg aattgcctgc cgtcggcggc ggcaaaaagc tggtgtatac cgggaaatac
    11101 ctgccgctga cgtcactcac gcagattcag gatcaagcac tggcggcgat tctggcgcgt
    11161 catcagggga tctggtccgg tgaggcggag caatatctgc tcactcatgc agaggcaatt
    11221 tcccatgatt aatcgcgcta cttaactctg catgtcgctg gctggtcgcc gcggcaactt
    11281 cgcgacctga tgtgcggctg attgctgaag cggcccaatg tgcacgagaa ggataaaaaa
    11341 acatcagcgc cttaaggggc gctcaaattg gtggcaaagt acacattgtt tatgccggat
    11401 gcggaataaa gccttattcg gcctacataa tctcgaaaat tcaatatatt gcagttatct
    11461 ggtaggcctg gtaagcgtag cgcatcaggc aatgctgtgt ttgtcatcag tctcagcgcc
    11521 cggtgcaata cgtgacatcg tgagccatgt cagtaagtgg ttaaattcgc agtggtagca
    11581 tgccttccgt gatttcttac taaccggaga agtaatggaa ctgcttttat tgagtaactc
    11641 gacgctgccg ggtaaagcct ggctggaaca tgcactgccg ctaattgctg aacagttgca
    11701 gggtcgccgc tcagcggtgt ttatcccttt cgctggcgta acgcagacct gggatgatta
    11761 cacagcgaaa acggctgcgg ttctcgctcc gctgggtgtt tctgtcaccg gtattcatag
    11821 cgttgtcgat cccgttgccg cgattgaaaa tgctgagatc gtgattgtcg ggggcgggaa
    11881 tactttccag ttgctgaaac agtgccgcga gcgcgggctg ctggcaccaa ttactgacgt
    11941 ggttaaacgt ggtgcgctgt atattggctg gagcgcaggc gctaaccttg cttgcccaac
    12001 cattcgtacc accaacgata tgccgattgt cgatccgcaa ggtttcgatg cgctaaatct
    12061 gttcccgctg caaatcaacc cgcacttcac caacgcgctg ccggaaggcc ataaaggtga
    12121 aacccgtgag cagcgtattc gcgaactgct ggtcgtcgcg ccagaactga cgattattgg
    12181 tctaccggaa ggtaactgga tcacggtgag taaaggtcac gctacgctgg gtggcccgaa
    12241 caccacttat gtgtttaagg ccggtgaaga agcggtgccg ctggaagcgg gtcaccgttt
    12301 ttaagtgaat tgccggatgc atgatccggc atcttgttca tagtgcctga tgcgacgctt
    12361 gacgcgtctt atcaggccta caggattcac aatattaata ctcgtcccgt ccttcatctt
    12421 catccggctg ttccagcaca ctgtaagcca ccgaacaaaa cagcgagttc agacgctgca
    12481 tatctcccag taagcctaaa tgtagcgaac tggtttcaat gctctgcacg ttttgctgat
    12541 gcaggcgatc gacgtgggcg tgggaatagc ggcgattaag aatgcgaaag cgatgcttgc
    12601 tgcggcgcaa acgacgagcg ctggtgacat cgccagagaa gaacaccgac attgccagct
    12661 ttaaattact gagcaattgc tcatagagcg catccagttc cttcaaccca tcaagcgaaa
    12721 atgcccgccg tgctgccagc gatttatcag caatttcgct gcccatgcgc tcgacgatat
    12781 cggaagcctg ttcaaggttg agcgacattt caataatctc cgcccagcga cgcgactctt
    12841 cttctgccag ttcctctttt ggcattcgcg ccagatacag cttaatggcg gtgtagagaa
    12901 cgttgatatc atctgccagc ttacgcagct ctttctcctg ccgtggctcg ccgtgcatca
    12961 ctttattcag cccttccatc atctgttcca tggcgtcgcc aatgcgcagg gtttcgcgcg
    13021 cggcgttcgc cagagcaagc gtgggcgtat ccagcgcgct gacatccaga tgtttaggcc
    13081 gtagctgggt atccagttcc ggttcatcgc gaataatcgt tttgcaaaac cgtgccatcg
    13141 ggtcaacaaa tggcagcatg accaggcaac gtacaaggtt gtagaagacg tggaaataga
    13201 tcaccagttc cgcttttggc aatgacaact tccccattgt ctctgccagc aaatggacaa
    13261 acggcaggat aatcaggcta cccaccagct taaacagcaa actacccagc gcgacacggc
    13321 gggctgcggc attggcggca ctgttgttga gcatcgccag caggccggaa ccgaggttag
    13381 caccaatcac cagacagagc gccacgggga aggagataat gcctgcggcg gtcagagtcg
    13441 cggtcagcag tacagcagca aggctggagt agctgataat ggcgaacatc gcgccaatca
    13501 gcgcatccag cagaatatcg ccggtcagcg aggcaaagat cacctgaacg ccgtttgcct
    13561 gggtgatcgg cgttacggcc tgcacaatca actccagcgc cagcaaaatc agcccaagac
    13621 caataccgac gcggcccagt tgcccggcgc gtgactgttt gcgtccgagg aaaaaaatca
    13681 cgccgataaa aataagtaac ggtgacagcc aggataagtc gaaggtgaga atacgcgcca
    13741 ttagcgccgt cccgacatct gcacccagca caatcaccag agccggtgcg agggctacca
    13801 gatcctgagc gacgaacgag gtcaccagca tggtggtggc attactgctc tgtaccagtg
    13861 cggtaacgcc gatccccgcg caaaaggcga gcggcttttt ttcgacgctc cggctaagga
    13921 cagtacgcaa acgcgcgccg aagacgcgca ttacgccggt tcgaacaata tgagtccccc
    13981 agaccagcag ggcgacggca gaaagcaggt gaagcagcgt taacacgagt tcaggttctc
    14041 cttatcgtta tatgttcctg aagcaattga atgaccaaag gaccggaaac acgctgactg
    14101 gcatctttac tgtgcctttt gacattcgtt tttccggcta agattattcc agcctgtttc
    14161 cagtataagt gtttcatatt aataaagaga cagggcactc attgagcgcc ctgtttgttg
    14221 taaggaaaag tgacagattt gtgaatcagt ccgcgtcata ccccagattc ggtgccagcc
    14281 agcgctcaac atcggagacg ctcatacctt tacggcgggc atagtcttca acctgatcgc
    14341 gctgaatttg cgccaccgcg tagtacttgc tgtccgggtg gctgaaatac caacccgaaa
    14401 ccgatgcacc aggccacatg gcgaaagatt ctgtgagttt catgccagta tgtttttcca
    14461 cttccagcag ctcccagatg gtggcttttt ccgtatgttc cgggcaggcc gggtagcccg
    14521 gtgccggacg gatgccctgg tagttttcgc ggatcagttc ttcgttgctg aggttctcgt
    14581 tcggcgcata gccccagtag actttacgca cacgctcatg gagatactcc gcaaaggctt
    14641 cggctaaacg gtcggcaagc gctttcacca tgattttgtt gtaatcatcg tgctgcgctt
    14701 caaaggcatc agccagtgcg tcctcttcca gcccgccagt cacggcaaat gcgccgatgt
    14761 aatctgcttt accagaaagc ttcggcgcaa cgaagtcagc gagacagtag ttagcgaatc
    14821 ccgttttttc ggtctgttga cgcagatggt ggctgacgtt gatcacatgg gtgcgcgttt
    14881 cgtcacggta gatttcaatg tcatcgccca cacggtttgc cgggaacaag cccaccacgc
    14941 cacgcggatt cagcgttttc tcggcgctta atttatccag catgtcgttg gcgtctttaa
    15001 acagccgctg cgcctcaacg cccaccactt catcttccag aatgcgcgga tacttcccgg
    15061 ccagcgacca ggtcataaag aacggtgtcc agtcgatgta attacgcaga gtttcgatgc
    15121 tggcttcaac ttcctgcacg ccgagacggt gcgccaccgg cggcgtgtaa gcctgccagt
    15181 caaaagcgaa gtcgttatcg cgcgccgctt ccagcgtgac cggtggtgtg cgcggtttct
    15241 tgcgcccgtg ctgaatacgt acggtttcat actccttgcg ggtacgagcg acaaaatcat
    15301 cgcgctgagt atcggaaagc agcgccgcca ccacaccaac ggtgcgcgag gcattctgca
    15361 catacaccgt cgggccgctg tagttctgct caattttcac cgccgtgtgc gcttttgagg
    15421 tcgtcgcgcc accgatcagt aacggaatgg tgaagccctg acgctccatc tctttcgcca
    15481 cgttaaccat ctcgtccagc gacggcgtga taagccccga aagtccaatc agatcagcat
    15541 tcacttcttt agcggtacgg agaatttttt ccgcaggcac cataacgccg agatcgacaa
    15601 tttcgtagtt gttacattgc agcaccacac caacgatatt tttaccgatg tcgtggacgt
    15661 cgcccttcac ggtggcgatc accatcttgc cgttggtttt gccctgctct ttgctggctt
    15721 caataaacgg ttcgaggtag gccactgcct gtttcatgac gcgcgccgat ttgaccacct
    15781 gtggcaggaa cattttcccc tcgccaaaca gatcgccgac cacgttcatg ccgtccatca
    15841 acgggccttc aatcacttca atcgggcgcg tagcctgctg gcgggcttct tcggtatcct
    15901 gctcgataaa ctcagtaatg cctttgacca gcgagtattc cagacgttta ttcacttccc
    15961 acgagcgcca ctccgcctgc tgggcgttag cggtgtcgtc ggttttgctg ccgcgatatt
    16021 tctcggcaag ctccagtaaa cgttcagtgc catcatcgcg acgattaaga atcacatctt
    16081 ccaccgcgtc gcgcagttca gcgggcaggt cgtcgtaaat cgccagttgc ccggcgttga
    16141 cgatccccat atccatgcca ttgcgaatag cgtagtagag gaacaccgcg tgaatggctt
    16201 cgcgcaccgg ttcgttgcca cggaatgaga aagaaacgtt agatacgccg ccggaaatca
    16261 gcgcgtgcgg cagttcgcgt ttgatgtctt cgcaggcgcc gataaagtcc tgcgcatagt
    16321 tgttgtgctc ttcaatgccg gtggcgaccg cgaagatgtt cgggtcgaag atgatatctt
    16381 ctggcgggaa gccaacctct tcggtgagga ttttgtacgc ccgacggcaa atctcgattt
    16441 tccgtgcgcg ggtatcggcc tgtccctgtt cgtcaaaggc cattaccacc accgctgcac
    16501 cgtaacgacg caacagtttc gcatgatgga taaaggcgtc gacgccctct ttcattgaga
    16561 tagagttaac aatgcctttg ccctgaatac acttcagccc cttttcgatg acctcccatt
    16621 ttgaggagtc gatcataatc ggcacgcgag cgatatccgg ttcaccggca atcagattga
    16681 gaaaacgcac catcgccgct tcggcgtcga gcatcccttc atccatattg atatcgataa
    16741 tctgcgcgcc gttttccacc tgctggcggg ccacatccag tgcctcgctg tatttctctt
    16801 ctttgatcag gcgcttgaac ttagcggaac cggtgacgtt ggtgcgttca cccacgttca
    16861 caaacaggct atcttcgcca atgttcagcg gctccaggcc ggacaaacgg caggctacgg
    16921 ggatttccgg cagtttgcgc ggcgctaatc cttctactgc acgactcatc gctgcaatat
    16981 gttgtggcgt ggtgccacag cagccgccga cgatattgag aaagcccgct tccgcccatt
    17041 cacgtatttg ttttgccatc gtgtcggcgt cgagatcgta ctcaccaaag gcgttgggta
    17101 gcccggcgtt cgggtgcgcg gtgacgtagc attccgcaat ccgtgacagc tcctgcacgt
    17161 actggcgcag ttcatcgggc cccagcgcac agttcaggcc aaaggtcagc gcttcggcgt
    17221 ggcgcaatga gttgtaaaat gcttcggtgg tctgcccgga gagcgtgcgc ccggaggcgt
    17281 cggtgatggt gccagagatc ataatcggca gctcaacgcc cagcgtttca aactccgttt
    17341 ttaccgcaaa taccgccgct ttggcgttaa gggtgtcgaa aacggtttca atcaggatca
    17401 gatccgcacc accttccacc agcgctttgg tggactctcg ataagccgcc accagcccgt
    17461 caaaagtgat attacgaaat gccggatcgt tgacgtccgg agaaatagac gccgtgcggt
    17521 tggttgggcc gagaacaccg gcaacgtagc gcggtttctc cggcgtgcgc gcggtccact
    17581 cgtcagcaca ggcgcgcgcc agtttcgccg ccgcaaagtt gatttccgcc gacagggatt
    17641 ccatctggta atccgccatc gcaatggtcg tggagttgaa ggtgttggtt tcgatgatat
    17701 ccgcgcccgc ttcaaagtag gcgttgtgga tagcggcgat cacttccggt ttactgagta
    17761 ccagcaggtc gttgttgcct ttgaggtcgc atggccagtc ggcaaagcgt tcaccacgaa
    17821 aatcggcttc gttcagtcga tagctctgga tcatggtgcc cataccgccg tccagcacca
    17881 gaatacgttc atttaactgc gcacgcagtt gttccacttt gctgctcaca cttgctcccg
    17941 acacgctcaa cccagattta ataaaaattc aacaaaccat actggcataa acgcatctgt
    18001 ggtaaaagcg accaccacgc aacatgagat ttgttcaaca ttaactcatc ggatcagttc
    18061 agcaactatt gcattaacta acaataaaaa tgaaaatgat ttccacgata cagaaaaagg
    18121 agactgtcat ggtcgcaccc attcccgcga aacgcggcag aaaacccgcc gttgccaccg
    18181 caccagcgac tggacaggtt cagtctttaa cgcgtggcct gaaattactg gagtggattg
    18241 ccgaatccaa tggcagtgtg gcactcacgg aactggcgca acaagccggg ttacccaatt
    18301 ccacgaccca ccgcctgcta accacgatgc aacagcaggg tttcgtgcgt caggttggcg
    18361 aactgggaca ctgggcaatc ggcgcacatg cctttatggt cggcagcagc tttctccaga
    18421 gccgtaattt gttagcgatt gttcacccta tcctgcgcaa tctaatggaa gagtctggcg
    18481 aaacggtcaa tatggcggtg cttgatcaaa gcgatcacga agcgattatt atcgatcagg
    18541 tacagtgtac tcacctgatg cgtatgtccg cacctatcgg cggtaaattg ccgatgcacg
    18601 cttccggtgc aggtaaagcc tttttagccc aactgagcga agaacaggtg acgaagctgc
    18661 tgcaccgcaa agggttacat gcctataccc acgcaacgct ggtgtctcct gtgcatttaa
    18721 aagaagatct cgcccaaacg cgcaaacggg gctattcatt tgacgatgag gaacatgcac
    18781 tggggctacg ttgccttgca gcgtgtattt tcgatgagca ccgtgaaccg tttgccgcaa
    18841 tttctatttc cggaccgatt tcacgtatta ccgatgaccg cgtgaccgag tttggcgcga
    18901 tggtgattaa agcggcgaag gaagtgacgc tggcgtacgg tggaatgcgc tgactttttc
    18961 tggcgggcag aggcaatatt ctgcccatca tacctgagtg gcaatagaat aagggtgtct
    19021 gttaatcgca ttgacgccaa aataacttaa tgtcatacac ttcacacact tttattaact
    19081 cagcattatt tttaaacatc aaaccactta attattaaca aaatcgcaag atctcattcc
    19141 accgaaactt ttacggctgc attatgagat tgagcgattt atgatatttc tttagttgta
    19201 atcatattaa attttttcac tgttctgata gttaaaattt aagacatcaa taaacaatga
    19261 gatatttaaa tgattactcg tattcctcgt agttctttct ctgcaaatat taataataca
    19321 gcccagacaa atgaacacca aaccctgagt gaattgtttt ataaagaact cgaggataaa
    19381 ttttctggca aggagctggc gacgcctcta ttaaaaagct tctcagagaa ctgtagacaa
    19441 aatggtcgtc atatctttag caacaaggat tttgtcatta aattttccac gtccgtctta
    19501 caagctgata agaaagaaat tacgataatt aataaaacga aaacacgaca ctcactcaaa
    19561 ccattgctcc aatatttgaa gaatacctaa tggaaatttt acctcaacgc tcagacactc
    19621 ttgataaaca agaattaaac ctaaaatcag atagaaaaga aaaagaattc ccaagaatta
    19681 aacttaatgg tcaatgttat tttccggggc gaccccaaaa ccgtatagta tgccgacaca
    19741 ttgctgcaca atatattaat gatatttatc agaatgttga ttacaaaccc catcaagatg
    19801 attattcttc agctgaaaaa tttctcactc acttcaacaa aaaatgcaaa aaccagactt
    19861 tggcgttgat ttccagccgt cctgaggggc gttgcgttgc tgcctgcggt gatttcgggc
    19921 tagttatgaa agcatatttt gacaagatgg aatcaaatgg catcagtgtt atggcagcca
    19981 tattactggt ggataaccat gctttgacgg tccggctaag aataaagaac acaactgaag
    20041 gatgtaccca ttacgtggtt tcggtttatg atcctaatgt aactaacgac aaaataagaa
    20101 tcatgagcga aagcaaagag gatattaaac actattctct gatggatttt atgaatgtag
    20161 attatagcct cctgaaatgg tcaaatgatc atgttattaa ccaatctgtt gcaataattc
    20221 cagcacttcc gaaagaacag ctattgatat taaaaggat
//
LOCUS       DAZPLI010000068        20128 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZPLI010000068 DAZPLI010000000
VERSION     DAZPLI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20128
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.069"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            201..872
                     /locus_tag="ACHUU3_002333"
     CDS             201..872
                     /locus_tag="ACHUU3_002333"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFR2082371.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            882..2078
                     /locus_tag="ACHUU3_002334"
     CDS             882..2078
                     /locus_tag="ACHUU3_002334"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR2082372.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIKRGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2078..2638
                     /locus_tag="ACHUU3_002335"
     CDS             2078..2638
                     /locus_tag="ACHUU3_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR2082373.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2640..3413
                     /locus_tag="ACHUU3_002336"
     CDS             2640..3413
                     /locus_tag="ACHUU3_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFR2082374.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3601..3876
                     /gene="frmR"
                     /locus_tag="ACHUU3_002337"
     CDS             3601..3876
                     /gene="frmR"
                     /locus_tag="ACHUU3_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFR2082375.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3911..5020
                     /gene="frmA"
                     /locus_tag="ACHUU3_002338"
     CDS             3911..5020
                     /gene="frmA"
                     /locus_tag="ACHUU3_002338"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFR2082376.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5113..5946
                     /gene="fghA"
                     /locus_tag="ACHUU3_002339"
     CDS             5113..5946
                     /gene="fghA"
                     /locus_tag="ACHUU3_002339"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR2082377.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6172..6711)
                     /locus_tag="ACHUU3_002340"
     CDS             complement(6172..6711)
                     /locus_tag="ACHUU3_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFR2082378.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSKQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6813..8024)
                     /gene="mhpT"
                     /locus_tag="ACHUU3_002341"
     CDS             complement(6813..8024)
                     /gene="mhpT"
                     /locus_tag="ACHUU3_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFR2082379.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8311..9324)
                     /gene="mhpE"
                     /locus_tag="ACHUU3_002342"
     CDS             complement(8311..9324)
                     /gene="mhpE"
                     /locus_tag="ACHUU3_002342"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFR2082380.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9321..10271)
                     /gene="mhpF"
                     /locus_tag="ACHUU3_002343"
     CDS             complement(9321..10271)
                     /gene="mhpF"
                     /locus_tag="ACHUU3_002343"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFR2082381.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10268..11077)
                     /gene="mhpD"
                     /locus_tag="ACHUU3_002344"
     CDS             complement(10268..11077)
                     /gene="mhpD"
                     /locus_tag="ACHUU3_002344"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFR2082382.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11087..11953)
                     /gene="mhpC"
                     /locus_tag="ACHUU3_002345"
     CDS             complement(11087..11953)
                     /gene="mhpC"
                     /locus_tag="ACHUU3_002345"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFR2082383.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11971..12915)
                     /gene="mhpB"
                     /locus_tag="ACHUU3_002346"
     CDS             complement(11971..12915)
                     /gene="mhpB"
                     /locus_tag="ACHUU3_002346"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFR2082384.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12917..14581)
                     /locus_tag="ACHUU3_002347"
     CDS             complement(12917..14581)
                     /locus_tag="ACHUU3_002347"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFR2082385.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLHVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14623..15456
                     /locus_tag="ACHUU3_002348"
                     /pseudo
     CDS             14623..15456
                     /locus_tag="ACHUU3_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
     gene            15533..16615
                     /gene="lacI"
                     /locus_tag="ACHUU3_002349"
     CDS             15533..16615
                     /gene="lacI"
                     /locus_tag="ACHUU3_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFR2082386.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16738..19812
                     /gene="lacZ"
                     /locus_tag="ACHUU3_002350"
     CDS             16738..19812
                     /gene="lacZ"
                     /locus_tag="ACHUU3_002350"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFR2082387.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            19864..>20128
                     /locus_tag="ACHUU3_002351"
     CDS             19864..>20128
                     /locus_tag="ACHUU3_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2082388.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT         4736 a         5518 c         5224 g         4650 t
ORIGIN      
        1 tacataccaa acatacaaac acttaacata tgaattgcat aaaaacatta cccagtcgaa
       61 cctccgggtc tgaaagccct tgcttttcag ttattagcaa catttaacat ttataaatta
      121 acatctggct gcgccagaga ttacgtctct gagtgcatcg ttgtgcttgt agtataataa
      181 ataaaatgga gtgtcattaa gtggataagg ttttagattc agccctcctt tcttcggcaa
      241 ataaaagaaa gggtatcctt gctattggcg cacatcctga cgatatagaa ttaggctgtg
      301 gcgcatcgct tgctcgtctt gcgcaaaaag gaatttatat cgcagcagtg gtgatgacta
      361 ccggcaactc tggtacagat ggaataatcg atcgccatga agaatcgcgc aacgccctaa
      421 agatattagg gtgccaccaa actattcatc ttaattttgc tgacacccgc gctcatttac
      481 agctcaatga tatgatttcc gccctggaag acatcattaa aaatcaaatt ccttctgatg
      541 ttgaaatcat gcgggtatat accatgcatg atgccgaccg ccatcaggat catctcgctg
      601 tttatcaagc ttcaatggtt gcctgccgca ctattccaca aattctcggc tacgaaaccc
      661 caagtacctg gctttcattt atgcctcagg tttttgaatc cgttaaagaa gaatatttca
      721 cagtcaaact tgctgcatta aaaaaacata aaagccagga acgacgtgat tatatgcgtc
      781 atgatcgcct gcgtgcagtt gcacaatttc gcgggcaaca ggtcaatagc gatctgggtg
      841 aaggctttgt aattcataaa atgattcttt gagcaaattg catgaaaacc tggatattta
      901 tctgtatgtc catagcaatg ttgctatggt ttttaagtac gctaagacgt aaacccagtc
      961 aaaagaaagg ctgtattgac gccattatac ctgcgtataa cgaaggcccg tgtctggcgc
     1021 agtcactgga taatctactg cgtaaccctt atttttgccg ggtaatttgc gttaacgacg
     1081 gctccacgga caataccgaa gcggtcatgg cggaagtcaa acgcaaatgg ggcgaccgct
     1141 ttgttgccgt cacgcaaaaa aataccggta aaggtggtgc gctgatgaat ggcctcaatt
     1201 atgccacctg cgaccaggtt tttttaagtg atgccgacac ctatgttccg cccgatcaag
     1261 acggaatggg ctatatgctg gcagaaatta agcgcggtgc tgatgccgta ggcggcattc
     1321 cctctactgc gttgaaaggc gcggggctgt taccgcacat ccgcgcgacc gtaaagttgc
     1381 cgatgattgt tatgaagcgc acgctacagc agctcctggg cggcgcaccg tttattatca
     1441 gcggtgcctg cggaatgttc cgtactgatg tattgcgtaa gttcggtttc tcggatcgta
     1501 ctaaagtcga agaccttgat ctcacctgga cattagtggc aaacggctac cgtattcggc
     1561 aggcgaatcg ctgcatcgta tacccacagg aatgcaacag tccgcgtgag gagtggcgtc
     1621 gctggcggcg ttggattgtg ggctacgcgg tctgtatgcg cctgcataaa aggcttttat
     1681 ttagccgctt cggtatcttc agtatatttc ctatgctgtt ggttgtgatt tatggcgttg
     1741 ggatttatct cactacctgg tttaatgaat tcatcaccac cgggccgcat ggtgtggtgt
     1801 tggcaatgtt tccgcttatc tggatcggcg tagtttgtgt tattggtgct tttagcgcct
     1861 ggtttcatcg ttgctggttg ttggtgcctt tagcgccgct ttccgttgtg tatgtattat
     1921 tagcttatgc catctggatt atctatggac ttattgcctt ttttactgga cgcgaacctc
     1981 agcgcgacaa acccacccgc tattccgcac tggtggaagc gtcaaccgct tattcccaac
     2041 cttctgtcac aggaactgaa aaactatctg aagcttaatg ttaaagagaa aaattattca
     2101 gattgcagac caggtaatta ttgatgaaac tgcaggtgaa gttgttatcg gcgcgaatac
     2161 ccgtatttgt catggtgccg ttattcaggg tccggtagtg attggcgcaa actgcctgat
     2221 aggtaattat gcgtttattc gtcctggcac aataatcagc aatggtgtaa aaattggttt
     2281 tgccaccgaa attaaaaatg cggttattga agcggaagca acgattggtc cgcaatgtta
     2341 tattgccgac tcggtagttg caaaccaggc atatttgggc gcacaagtac gtaccagtaa
     2401 tcatcgtctg gatgaacagc ctgtgtctgt tcgaactcca gagggaatta tcgctaccgg
     2461 atgcgataaa ttaggttgtt atatcgggca gcgttcacgc cttggcgtac aagttattat
     2521 tttgcctggg cgaattattt ccccgaacac gcaacttggc ccgcgcgtga ttgtagaacg
     2581 taatttacct accggaactt attcactccg acaagaactt atccgtacag gagattaaaa
     2641 tgataaaacg gacgttatta gcggcggcca tttttagcgc attgcccgct tatgccgggt
     2701 taacttccat taccgcgggc tacgatttta ccgattattc tggcgatcat ggcaaccgta
     2761 atttagcgta tgctgaactg gtggcgaaag ttgaaaacgc aacgctgctt tttaatcttt
     2821 cccaggggcg tcgtgattat gaaactgaac atttcaatgc cactcgcggt cagggtgcgg
     2881 tctggtataa atggaataac tggctgacaa cccgaacggg tattgccttt gcggataata
     2941 cgccggtctt tgcccgccag gattttcgtc aggatattaa cctggccctg ttgccaaaaa
     3001 cgcttttcac gaccggttat cgctacacta aatattacga tgatgtcgaa gtcgatgcct
     3061 ggcaaggcgg cgtatcactc tatactggcc cggtgatcac cagctaccgc tatacccatt
     3121 atgactccag cgatgcaggt ggtagttata gcaatatgat ttccgtgcgt ctgaatgacc
     3181 cgcgcggcac tggttatacg caactatggc taagccgcgg aacaggcgct tacacctatg
     3241 actggacgcc agaaacacgc tacggcagca tgaagagcgt cagtttgcaa cgtattcaac
     3301 cgctgactga gcaacttaat ctcgggctga cggcaggtaa agtgtggtac gacaccccaa
     3361 ccgatgatta taacggtctg caacttgcag cccatctgac ctggaaattc tgattgcttc
     3421 tgccgcccgc tatccggggc ggccttccct gccgattagc cccactccct ttcctcgttg
     3481 ttttccgacc acattcaccg gataaatttt attctccagc gttatatact atagggaggt
     3541 atgcattgac atatagcata cctccctata gtatattgcg tgcagatgat gaggtgcgaa
     3601 atgcccagta ctccggaaga gaagaaaaag gtccttactc gagttcgtcg tattcggggg
     3661 cagattgatg ctctggaacg gtcgctggag ggtgatgccg aatgccgtgc catactccaa
     3721 cagatcgctg ccgttcgggg cgcggctaac gggctgatgg cagaagtgct tgaaagccat
     3781 atccgggaaa cgtttgaccg aaatgactgc tacagccgcg aagtcagcca atccgttgac
     3841 gacactattg aactggttcg agcctatctt aaatagctga atctattacc atattgagga
     3901 agagcgagag atgaaatcac gtgctgccgt tgcatttgct cccggtaaac cgctggaaat
     3961 cgttgaaatt gacgttgcac caccgaaaaa aggtgaagtg ctgattaaag tcacccatac
     4021 cggcgtttgc cataccgacg catttaccct ctccggggat gacccggaag gtgtattccc
     4081 ggtggttctc ggtcacgaag gggccggcgt tgtggttgaa gtcggtgaag gcgtaaccag
     4141 cgtcaaacct ggcgaccatg tgatcccgct ttacaccgcg gagtgcggcg agtgtgagtt
     4201 ctgtcgttct ggcaaaacta acctctgtgt tgcggttcgc gaaacccagg gtaaaggctt
     4261 gatgccagac ggcaccaccc gtttttctta caacgggcag ccgctttatc actacatggg
     4321 gtgctctaca ttcagtgaat acaccgtggt cgcggaagtg tctctggcca aaattaatcc
     4381 agaagcaaac catgaacacg tctgcctgct gggctgtggc gtgaccaccg gtattggcgc
     4441 ggtgcacaac acagctaaag tccagccagg tgattctgtt gccgtgtttg gtcttggcgc
     4501 gattggtctg gcagtggttc agggcgcgcg tcaggcgaaa gcgggacgga ttatcgctat
     4561 cgataccaac ccgaagaaat tcgatctggc gcgtcgcttc ggtgctaccg actgcattaa
     4621 cccgaatgac tacgacaaac cgattaaaga tgtcctgctg gatatcaaca aatggggtat
     4681 cgaccatacc tttgaatgca tcggtaacgt caacgtgatg cgtgcggcgc tggaaagtgc
     4741 gcaccgcggc tggggtcagt cggtgatcat cggggtggca ggttctggtc aggaaatctc
     4801 cacccgtcca ttccagttgg tcactggtcg cgtatggaaa ggttccgcgt ttggcggcgt
     4861 gaaaggtcgt tcccagttac cgggtatggt tgaagatgcg atgaaaggtg atatcgatct
     4921 ggaaccgttt gtcacgcata ccatgagcct tgatgaaatt aatgacgcct tcgacctgat
     4981 gcatgaaggc aaatccattc gaaccgtaat tcgttactga tttcccgcag gtataccccg
     5041 tccacttcag acggggttct taatactctc cccgggcagc cgtccggggg attaaccgtg
     5101 agataatgac tgatggaact cattgaaaaa catgccagct ttggcggctg gcaaaatgtg
     5161 tatcggcatt attcccaatc actgaaatgt gaaatgaatg tcggcgtcta tctcccacca
     5221 aaagccgcga atgaaaaatt gccggtgctg tactggcttt caggcctgac ctgcaacgag
     5281 cagaatttca ttactaaatc ggggatgcag cgttacgcgg ctgagcacaa cattattgtt
     5341 gttgcgccgg acaccagtcc gcgaggcagt catgtcgcag atgccgaccg ttacgatctc
     5401 gggcaaggtg ccgggtttta cctgaacgca acgcaagcgc cgtggaatga acattacaaa
     5461 atgtatgact atatccgcaa cgagctgccg gatttagtga tgcaacattt tccggcaacg
     5521 accagaaagt ctatctctgg tcattctatg ggcgggctgg gcgcgctggt gctggcgtta
     5581 cgtaacccag atgaatatgt cagcgtctcg gcgttttcgc ccattgtctc cccatcgcaa
     5641 gtaccgtggg gacagcaagc ctttgccgca tatcttggtg aaaataaaga tgcctggttg
     5701 gattacgatc cggtgagtct tatttcacaa ggtcaacgcg ttgcggaaat catggttgat
     5761 caggggttga gtgatgattt ttacgcggaa cagctgcgga ctccaaatct tgaaaagatc
     5821 tgccaggaga tgaatatcaa gacgttaatc cgttatcacg agggttacga ccacagctat
     5881 tattttgtct ccagttttat tggcgagcat attgcctacc acgccaataa actgaatatg
     5941 cgttgataat agtgcacgac tgccggatgc ggcgtgaacg ccttatccgg cctacacttc
     6001 gcccgtaaac cgtaggcctg ataagacgcg caaagcgtcg catcaggcat ggcgcacgac
     6061 tgccggatgc ggcgtgaacg ccttatccgg tctacacttc gcccataaac cgtaggcctg
     6121 ataagacgcg catagcgtcg catcaggcat ctgcgcacga cttatctatg tttaccacat
     6181 caaatcatca ggcactttga agtcggcata cggatcgtct tcatcctgct cttcggcact
     6241 gagcgcgctg tgcaacacaa tactgctggc atcacgctga gcaattttat cggcaacact
     6301 ggcgggaata atggtgtatt caccttcgct attgttatcg accaacaagc gggcaatcgc
     6361 cagacgacca ttaatcaact gcgcctgtgt gagcttatcg acaaaaatct tcttaatcag
     6421 attgccgtca gtgaagttaa aaccaatatc gccattggca atggtgattc ggttcatttc
     6481 aatgagctgc ttcacctggg ctttatattc tttcgccaac gccgcttgtt tttgctgttt
     6541 gctaagctgt ttatcacgct caagctgtgc ctttttattt tcttctaccg cagcccgagc
     6601 ttcacgcgcc tgaacgcgcg attttttcgc cgttctctcg accttcgccg cttttttgct
     6661 ggtgactaat cctgctttga gcaactgctc ttgtaaggta agttttgcca tttttgtttc
     6721 caaacccgct gaataatttt tagcgagtat acctgtaaac gccgtgggtg tattgggggc
     6781 aagtcccttt cgcccctgct taataaggcg cgtcaggcat cggcacacgg ctgcattctt
     6841 gatctccgac tcatcaaaat aaacaccgcc aacccagcaa caagaatacc cggtgcagaa
     6901 gcggccatta cgccgaccgt gccagtgcct aatgccagca ttttcccggc cagtaacgga
     6961 ccgctcatag cccccagacg ccctaccgcc acggctgttc ccacacctgt tgcgcggatc
     7021 tgcgaactgt aaaacaacgg tgccagggca tacaaaacgc tttgcccacc tgtcgcaaac
     7081 aaccccgcga caaatcccgc cagcaacata ccgttaaatg acgacaccgt tccaagcgcc
     7141 agcagcgaag ctaacatgcc gctataaatc agtagcgaca tggttactgg acgcagctta
     7201 tccatcaatg cgcccaacat taacgtcccg cttgccgccc ccatttgcag ggcaaacatc
     7261 acccctgccg cctgcgatgg ctggaatcct tgctccacca aaagtagcgg tagccagttg
     7321 atcaacatat aaaccaccag cagagtgaag aaataacaca accacagcag cagcgtcgcg
     7381 gttgccgttt ctggcgcaaa taaggcacgc agtggtggcg cagcctgttt ttcgccagcg
     7441 aaaaccgctg actccggcag ccagcgcatt aatagcggca ccagaatcaa cggcaccaca
     7501 ccacctaccc aaaacaccgt ttgccatgct aagtttgccc ccgcgaaacc cagtgtcgcc
     7561 gccagcgccg cgccaatggg aacaccgcaa tacatcaggc tcactgccgt cccacgaaaa
     7621 cgtggacccg cggcttcaga tgtcagggcg ataagattcg gcaacgccgc ccccagcccg
     7681 acaccggtca tcagccgcgc aaagaccagt gaggggaaat cccaggcaat cgccgttgcc
     7741 agtgagaaca aaccaaacag cgcaactgag ccaatcaaaa tacgcttacg accataacgg
     7801 tctgccagca tcccgccaac caacgcgccg ggtagcaaac cgagtattcc ggcgctaaat
     7861 atccagccca tttgcatttt atcgagtgcg aaagcctggg cgatgccacc cgccgcaatg
     7921 ccagccgcct gaagatccag cccttccatc agagcgacca aaaaacaaag cccgatggtc
     7981 agcatcaggc gggatgaaga tgatgaaggg gtacgagtcg acataagatg aacctcattg
     8041 cgagtgcagg tgaatcgcga ctgatacatt gccagccgcg taatcagaac tatttaatga
     8101 ctgcctacgg gcgaaatgta gcgaaatgta ggccggataa ggcgttcacg ccgcatccgg
     8161 cagtcgtgcg cagatgcctg atgcgacgct aacgcgtctt atcagaccta caaattctca
     8221 cgccatccgt aggccggaca aggcgttcac gccgcatccg gctgtcgtgc tgggatgggc
     8281 atacaaaatg cacgttatcg gcagtcatca ttatttgttg ttgcgcagat ccagcgccac
     8341 gtcgacgatc atatcctcct ggccgccaac catccggcgt ttgcccagct caacgagaat
     8401 atccaccgca cttaagccat aacgcgccgc cgccgtttca cagtgacgca ggaagctcga
     8461 gtaaacacca gcgtatccca gcgccagcgt ttcgcgatcg actcgtaccg gacgatcctg
     8521 caacggacgc accaggtcgt cggcggcatc cattaacgca tagagatcgg tcccatgctg
     8581 ccagcccagt ttatccgcgg cggcaataaa cacttccagc ggtgcgttac ctgcgcccgc
     8641 gcccattccc gcgaggctgg cgtcgattcg gtcgcagccc tcttccaccg ccgcgataga
     8701 gttcgccacg ccaagactca ggttatggtg agcgtgcatg ccggtttgcg tttctggttt
     8761 caacactgct ttcagggcgc ggaaacggtc acggatatcg ctcatgttca tcgcaccgcc
     8821 agaatccacc acataaatac aggtcgcacc gtagccttcc atcagctttg cctgcttggc
     8881 gagattctcc ggcgtggtca tatggctcat catcagaaaa ccaacggtgt ccattccgag
     8941 ctcgcgggca tactgaatat gctgggcgga aacatcagct tcggtacagt gcgttgccac
     9001 acgaaccacc cgcgcgccag cctgccaggc atttttcaga tcgtgaatag tgccgattcc
     9061 tggcagcaac aacgtcgcga ttttggcgtg cctcaccaca tccgccgccg cttcaatcca
     9121 ttcaaggtcg ctatgtgcgc cgaaaccata gttaaagctg gaaccttgca aaccgtcgcc
     9181 gtgggccact tcaatcgaat ccacgcgggc atcgtccagt gctttggcaa tctggcgaac
     9241 gttttccagc gaatactgat gacgaatggc gtgcataccg tcacgcaatg tgacgtccga
     9301 gatataaagt tttttaccgt tcatgccgct tctcctgcct tgcgcgccag tgactgggcc
     9361 attttttccg ctgtcgccag cgcactggaa gtcataatat cgaggttgcc cgcataggca
     9421 ggcagataat gcgctgcgcc ttcgacttcc agccagaccg ctgttttcag tccggagaat
     9481 tgccccacgc ccggtaaatt gaccggttta tcctgcggga taacttcaaa ctgcacgcgc
     9541 tgtttcaggc gataacccgg tacgtaagcc tgcaccgcct cagccatttc attgattgag
     9601 gcttcgatat catcttgtga agcttcgtcg ctcaatacat acaccgtgtc acgcatcatc
     9661 aacggtggct ctgctgggtt aagcacaata atcgccttcc cttttgccgc gccgcccacc
     9721 acttcaatgg ctcgggaagt ggtttccgta aattcatcga tattggcacg cgtgccaggt
     9781 ccggcagatt tactggcgat agaagcgata atttcggcgt aatgaacacg cgccacgcgt
     9841 gaaactgccg ccaccattgg aatggtggcc tggccgccgc aggtgaccat gttgacgttc
     9901 agttgatcga cgttcgcctc gaggttaacc accggcacgc agtaagggcc gatggcagca
     9961 ggcgtcaggt caattaagcg aatatccggt ttcgcttcgc gtaaagcggc atcgtttttc
    10021 acatgcgcgc ccgcgctggt cgcatcaaat acaatgtcga tatcagcaaa ttcaggcatg
    10081 ttcatcagcc cgatcacccc ttcatgggtg gtggcgacgc ccatacgtct ggcacgcgcc
    10141 agaccgtcgg actgaggatc aatgccgacc atcaccgcca tctccagatg ctgaccgtga
    10201 cgcaaaattt taatcatcag atcggtgcca atgttgccag aaccgataat ggcgacttta
    10261 cgcttactca tgacagactt ccttttgggg ctgcgcttga aaatgtcgca gcaactgaac
    10321 ctatgccttc aatatgggct tcaaaacgat cgcccgcatt caccgccacc atcgggccta
    10381 atgccccggt aagaatgata tctccggcgc gcagcggttc acccagactg gccattttgc
    10441 gtgccagcca gacggcggca ttaagcggat gtcccaggca ttcgctgccg cgcccgctgg
    10501 aaacctcttc gttattacgc gtcatcttca tggcgcagtt tttcaggtct aaccccgccg
    10561 gacgttgcgc cggaccgccg atgacataca ccccgcagga agcgttatct gccacggtat
    10621 cgacaaactg aatcgaccag tcgcgaatgc ggctccccac cacttccagc gccggaagta
    10681 cccattcaat ggcgttatac agttcgtcga aggtgatatc agttgcgggt aaatcgcggt
    10741 tcaacaccag tgcgatctcc gcttcaatgc ggggttgcag aacacgggaa aaaggaatga
    10801 tttcgttatc gccataacac atgtcggcaa ataacgtccc aaaatccggt tgatcaacgc
    10861 ccagttgttg ttgcactttc ggatgcgtca ggcccacttt acgccctacc acgcgacgcc
    10921 cctgcacaac gtcatattgc acatttatgt gctgaatggc gtaggccgct tcagcgtgat
    10981 cgataccaat cagatcgcgc agcggcgcaa tcgcttcgcc ctgctctgcg gcgcggcgta
    11041 aatccgccgc cagttgctca agagtatgct tcgtcatgac cattccttaa gggcgtgcga
    11101 ggaaattcag caccaattga ttgaaagcgt cggcatgttc ccactgcgcc cagtgaccac
    11161 agtcgcggaa gatatgcagt tcagaaccgg caatgccgga cagcagacgc agacccgcat
    11221 ccatcggcac aaagcggtcg ttgcgccccc agacaatcag ggtttgcgct ttgatttccg
    11281 ccagacgtgg gccaaaatcc gggaactgtt tcggattagc ttccaggctc ttaacgaagt
    11341 tttccaggtg atcgcggcgc gacagcatat tattcaggcg cgcttcaaac agggcgtcgg
    11401 tcaaatcgct ggtatcgaaa acgaagatat ccatcatcag cttcaggttt tcgatagtcg
    11461 gctgacgata aagctgattc agtcgcttaa taccttcggt tggcatcggc gtaaacaaac
    11521 tcatgccgcc cgtaccgccg cccatcagca ccagtttgcc gacgcgctcc ggccagttaa
    11581 gggtgaacgc cacagaacta tggccgccca tcgagttgcc cagcaggtgg attttggcga
    11641 tatccagttg atccaccacg cttttcagga ttcgtgcatt aagatccgat cgtgaaccac
    11701 tattaacgat cgaatcgctt ttgccccaac ccggacaatc cagcaggatc acccgatagc
    11761 ccgcctctac cagcggatcg atattgcggc tgaagttcgc ccagccagta gcacccgggc
    11821 cggaaccatg cagcaggaca acggtttcgt cgccttgtcc gcagtcatta aaatggatgc
    11881 gcagcgtttt acccgcttct tctacattca gaaaacggct ggtggcggct tcggtttgtg
    11941 gctgataact catcatcttc tcctgcatat tcagttctct gttctggcgc ttaacgagcc
    12001 aaatccggca atccactccg ggattgggcg gtaataacgc ccttcgctac gccagttgcc
    12061 aaatgcagaa atagcggcaa aagcggcgac ccaggttttg atttcatgtg tcgactttcc
    12121 ggcaatggcg gaaagctctt cgttactgac ggcatccagt tcctgtatgc gtccctgctc
    12181 cagcaaagtc atgaactggt tatcccaaat cgggttgagc ggatgcagag ttctctgatc
    12241 ctcaacaaac ttctcagcgg cgctaatcac cctttgctga cgcaattcgc gctcactggc
    12301 gggtaaatct ttcccgctcc ccaacagacg gtcgcgcata tgggcatcgg ctttcgccag
    12361 ttcgggcacc ggcggctgat gggaaagccc accggaaccc aggaacagca cgcgtttatt
    12421 gagagtgctg gtgaaacgtc caatggcttc acccaacatg cgggtacgct ggaaaccggg
    12481 cagcggcgtg gcgacaccgt tgatgaacac aggcagaact ggcaccttat ccagcccacc
    12541 gagcaggaac tccagcggct gggcgaaccc gtggtccacc tgcatacagt aagaaacggc
    12601 aagatcgatc ccgctcttca tgacagcatg cgcacaggcc tccgccagct ccacaggcac
    12661 gggcagctct cctgccgcac tgccgaaatc accaattgcc gtcgctccaa cgcctaaaca
    12721 gaacggtggc atcacgtcat agaaaaagcc gttgtagtga tctggcgcaa acagcaccac
    12781 cagttcaggg gagaaagccg caatacgctc gcgggcgctg gcaatcacgc cattgacctc
    12841 atcgagtacc tcttgcgccg ggtcgacata ccccaccaac ggcgagtggg aaagacagtg
    12901 aagataagcg tgcatatcag gctacctttt cgacagaaac gtcggcatca gggcgggtca
    12961 gcgtcatcac cgacgccagt ttattcagcg tattgcccag ggtttgcgga atggcggtgg
    13021 cggcaacaaa gcgatccggg cgcatcacca ccagcgaagc attgtgttgc gcgaaccagc
    13081 tacgcagacg accttgcgta tcgcccacgt gtagcacgcc gtcgtggtta tcctgtgcgg
    13141 tatgaatttg cacttccggc accacctgaa tgaagcgtgt gcccaacgcg cgccactgct
    13201 ggatttgctc gtcgctcatc ccccacagtg gattgcatcc ccagccaatt accgcgaagt
    13261 tcgcgccgat cgcgttatcg agcagcgtca cgtcgccgtt ttccagcgtg actttcggct
    13321 gaataaacat cttgccgacc ggagagtgct tcgcttcgcc ctcacgcacc agcgcaccgc
    13381 cgtaatattg cggcatcggc ttgaagcgca tttcgaggaa gtagcgtttt actggcggca
    13441 gataattcaa cagccaggaa acgccgtcac gtaacgtacc ctgccagcgt ttcggcggag
    13501 ccagcacgtt gcccgccgtc acggacaggt caatcatcgc tttggcgtga tcgcgacgtt
    13561 cttgttgata ggtatcgagc agcgcatcgc gggctttccc ctggataacc aacgccagtt
    13621 tccaggcgag gttaaaggcg tcgcgcatac cactgttata gccctgcccc tgccacaccg
    13681 gcatgatgtg cgcggcatcg cccgccagca gtacgcgatc aatacggaaa cgttgcgcca
    13741 gtcgcgcgtt atgggtgtag acacgctggc gaatcaattc aacattgtcc ggattaggca
    13801 gcactttgct taacagcttg cgcatatttt gcggctcacg cagctgctct tcggtttctc
    13861 ccggcatcac cataaattca aagcgacgta ccgcatgagg cagcgcggca gaaacatacg
    13921 ggcgcaccgg atcgcaacac aaatagatat gcggcgtact taacggatcg ttggcgatat
    13981 ctaccacaat ccactgattt ggcgcagttt taccttcaaa cggcacattc agggtgcgac
    14041 gaacaaaact tgccccacca tcacaggcca ccagccactg ggctttgatc gtttcccgct
    14101 gcccttctgc cgttttcagg tgcaaggtca cttcgtcatc ttgctggctg aaggcctcca
    14161 gctcgcggga aaacaagcag cgcacattcg gaaaacgcga caacccttcc agcatcaccg
    14221 catcgacctg tggctgaata aaggcgttac ggcgcggcca gccaaattca tcggtcattg
    14281 gctgaatatc agcaaaacag cggccttttg gggtgagaaa acgcatcgcg tgccacggtg
    14341 tagtgtgcgg cagaacattc tcgaccaggc cgaccgactg catggtgcgc agcgcctcgt
    14401 catcaatacc aatcgcacgc gggtagtcga tcaacttatc gagtttctcc accaccagca
    14461 cgtcaatgcc catctgaccg agatagttcg ccatcatcag cccaaccgga ccggcaccag
    14521 cgatcgccac ctgaacgcta tggttaacag caggctggat gtcagggtgt tgtattgcca
    14581 tttctgcaca acgttgtttt aactatagaa atgtcaatta atatgcagaa caatgagcag
    14641 acggaataca aaaccgtgcg cggcttaacc cgcggtctaa tgttattaaa tatgttaaat
    14701 aaacttgatg gcggtgccag cgtcgggctg ctggcggaac tcagcggcct gcatcgcacc
    14761 actgtgcggc gactgctgga gacgctgcag gaagagggat atgtccgccg tagcccctcc
    14821 gatgacagtt ttcgtctgac catcaaagtg cggcaattaa gcgaaggatt tcgtgacgaa
    14881 cagtggattt ctgcactggc ggccccgctg ctgggcgatc tgttgcgcga agtggtatga
    14941 ccaacagatg tgtccaccct ggatgttgat gcaatggtgg tacgcgaaac cacccaccgt
    15001 ttcagccgct tatcctttca ccgggcaatg gtcgggcgac gtttaccgct cctgaaaacc
    15061 gcctcgggcc tgacctggct ggccttttgc ccggaacaag aacgcaagga attaatcgaa
    15121 atgttagccg cccgccccgg tgatgactat caactggcac gagaaccgtt aaagctgcaa
    15181 gccattctgg cgcgcgcgcg caaagagggt tacggacaga actaccgcgg ctgggatcag
    15241 gaggagaaga tcgcctctat cgccgtaccg ctgcgcagtg aacaacgggt gattggctgt
    15301 ctgaatctgg tgtatatggc gagcgcaatg accattgaac aggcagcgga aaagcatctt
    15361 ccggcgctac aacgggtagc aaaacagatc gaagaagggg ttgaatcgca ggctattctg
    15421 gtggccggac ggcgaagcgg cgtgcattta cgttgacacc atcgaatggc gcaaaacctt
    15481 tcgtggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc
    15541 agtaacgcta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttctcgcgt
    15601 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
    15661 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
    15721 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
    15781 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
    15841 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
    15901 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
    15961 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
    16021 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
    16081 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
    16141 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
    16201 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
    16261 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
    16321 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
    16381 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
    16441 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
    16501 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    16561 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    16621 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    16681 cggctcgtat gttgtgtgaa attgtgagcg gataacaatt tcacacagga aacagctatg
    16741 accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
    16801 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
    16861 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt
    16921 gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag
    16981 gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac
    17041 accaacgtga cctatcccat tacggtcaat ccgccttttg ttcccgcgga gaatccgacg
    17101 ggttgttact cgctcacatt taatattgat gaaagctggc tacaggaagg ccagacgcga
    17161 attatttttg atggtgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt
    17221 tacggccagg acagccgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga
    17281 aaaaaccgcc tcgcggtgat ggtgctgcgc tggagtgacg gtagttatct ggaagatcag
    17341 gatatgtggc gaatgagcgg cattttccgt gacgtctcgt tgctgcacaa accgagcaca
    17401 caaatcagcg atttccatgt tgccactcac tttaatgatg atttcagccg cgcggtactg
    17461 gaggcagaag ttcagatgta cggcgagctg cgcgatgagc tgcgggtgac ggtttctttg
    17521 tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat
    17581 gagcgtggtg gttatgccga tcgcgtcacg ctacgtctga acgtcgaaaa cccggcgctg
    17641 tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgacgacggc
    17701 acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt
    17761 ctgctgctgc tgaacggcaa gccgttgctg attcgcggcg ttaaccgtca cgagcatcat
    17821 cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag
    17881 cagaacaact ttaacgccgt gcgttgttcg cattatccga accatccgct gtggtacacg
    17941 ctgtgcgacc gcttcggctt gtacgtggtg gatgaagcca atattgaaac ccacggcatg
    18001 gtgccaatga atcgtctgac cgatgatccg cgctggttac ccgcgatgag cgaacgcgta
    18061 acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat
    18121 gagtcaggcc acggcgctaa tcacgacgca ctctatcgct ggatcaaatc tgtcgatcca
    18181 tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc
    18241 ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc
    18301 aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac
    18361 gcgatgggta acagtcttgg cggcttcgct aaatactggc aggcgtttcg tcagtacccc
    18421 cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa
    18481 aatggcaatc cgtggtcggc ttacggcggt gattttggcg atacaccgaa cgatcgccag
    18541 ttctgcatga acggtctggt ctttgccgac cgcacgccgc atccagcgct gacagaagca
    18601 aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc
    18661 gaatacctgt tccgtcatag cgataacgag cttctgcact ggtcggtggc gttggatggc
    18721 aagccgctgg caagcggtga aatgcctctg gatgttgctc cacaagataa acagttgatt
    18781 gaactgcctg aactaccgca gccggagagc gccggacaac tctggcttac ggtacacgta
    18841 gtgcaaccga acgcgaccgc atggtcagaa gccggacaca tcagcgcctg gcagcagtgg
    18901 cgtctggcgg aaaacctcag cgtgacactc cccgccgcgc cccacgccat cccgcaactg
    18961 accaccagcg aaacggattt ttgcatcgag ctgggtaata aacgttggca atttaaccgc
    19021 cagtcaggct ttctttcaca gatgtggatt ggcgctgaaa aacaactgct gacgccgctg
    19081 cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc
    19141 attgacccga acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca
    19201 gcgttgttgc agtgcacggc agatacactt gccgacgcgg tgctgattac gaccgctcac
    19261 gcgtggcagc atcagggaaa aaccttattt atcagccgga aaacctaccg gattgatggt
    19321 agtggtcaaa tggcgattac cgttgatgtt gaagtagcga gcgatacacc gcatccggct
    19381 cggattggcc tgacctgcca gctggcgcag gtagcagaac gggtaaactg gctcggatta
    19441 gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg
    19501 ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg
    19561 acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt caatatcagt
    19621 cgctacagcc aacaacaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa
    19681 ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc
    19741 ccgtcagttt cagcggaatt ccagcttagc gccggtagct accattacca gttggtctgg
    19801 tgtcaaaaat aataataacc gggcaggcca tgtctgcccg tatttcgcgt aaggaaatcc
    19861 attatgtact atttaaaaaa cacaaacttt tggatgttcg gtttattctt tttcttttac
    19921 ttttttatca tgggggccta cttcccgttt ttcccgattt ggctacatga catcaaccat
    19981 atcagcaaaa gtgatacggg tattattttt gccgctattt ctctgttctc gctattattc
    20041 caaccgctgt ttggtctgct ttctgacaaa ctcgggctgc gcaaatacct gctgtggatt
    20101 attaccggca tgttagtgat gtttgcgc
//
LOCUS       DAZPLI010000069        20041 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZPLI010000069 DAZPLI010000000
VERSION     DAZPLI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.070"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            79..213
                     /locus_tag="ACHUU3_002352"
     CDS             79..213
                     /locus_tag="ACHUU3_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR2082389.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            192..296
                     /gene="speFL"
                     /locus_tag="ACHUU3_002353"
     CDS             192..296
                     /gene="speFL"
                     /locus_tag="ACHUU3_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR2082390.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            607..2805
                     /gene="speF"
                     /locus_tag="ACHUU3_002354"
     CDS             607..2805
                     /gene="speF"
                     /locus_tag="ACHUU3_002354"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFR2082391.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2802..4121
                     /gene="potE"
                     /locus_tag="ACHUU3_002355"
     CDS             2802..4121
                     /gene="potE"
                     /locus_tag="ACHUU3_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR2082392.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4177..5817)
                     /gene="pgm"
                     /locus_tag="ACHUU3_002356"
     CDS             complement(4177..5817)
                     /gene="pgm"
                     /locus_tag="ACHUU3_002356"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR2082393.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5843..6388)
                     /gene="seqA"
                     /locus_tag="ACHUU3_002357"
     CDS             complement(5843..6388)
                     /gene="seqA"
                     /locus_tag="ACHUU3_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR2082394.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6573..7337
                     /gene="ybfF"
                     /locus_tag="ACHUU3_002358"
     CDS             6573..7337
                     /gene="ybfF"
                     /locus_tag="ACHUU3_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR2082395.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7477..7770
                     /gene="ybfE"
                     /locus_tag="ACHUU3_002359"
     CDS             7477..7770
                     /gene="ybfE"
                     /locus_tag="ACHUU3_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR2082396.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7910..8440
                     /gene="fldA"
                     /locus_tag="ACHUU3_002360"
     CDS             7910..8440
                     /gene="fldA"
                     /locus_tag="ACHUU3_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR2082397.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8650..8736
                     /gene="uof"
                     /locus_tag="ACHUU3_002361"
     CDS             8650..8736
                     /gene="uof"
                     /locus_tag="ACHUU3_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR2082398.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8729..9175
                     /gene="fur"
                     /locus_tag="ACHUU3_002362"
     CDS             8729..9175
                     /gene="fur"
                     /locus_tag="ACHUU3_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR2082399.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9260..9586)
                     /gene="chiQ"
                     /locus_tag="ACHUU3_002363"
     CDS             complement(9260..9586)
                     /gene="chiQ"
                     /locus_tag="ACHUU3_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR2082400.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9636..11042)
                     /gene="chiP"
                     /locus_tag="ACHUU3_002364"
                     /pseudo
     CDS             complement(9636..11042)
                     /gene="chiP"
                     /locus_tag="ACHUU3_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
     gene            complement(11620..13284)
                     /gene="glnS"
                     /locus_tag="ACHUU3_002365"
     CDS             complement(11620..13284)
                     /gene="glnS"
                     /locus_tag="ACHUU3_002365"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR2082401.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13543..15489)
                     /gene="nagE"
                     /locus_tag="ACHUU3_002366"
     CDS             complement(13543..15489)
                     /gene="nagE"
                     /locus_tag="ACHUU3_002366"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR2082402.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            15822..16622
                     /gene="nagB"
                     /locus_tag="ACHUU3_002367"
     CDS             15822..16622
                     /gene="nagB"
                     /locus_tag="ACHUU3_002367"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR2082403.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16682..17830
                     /gene="nagA"
                     /locus_tag="ACHUU3_002368"
     CDS             16682..17830
                     /gene="nagA"
                     /locus_tag="ACHUU3_002368"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR2082404.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            17839..19059
                     /gene="nagC"
                     /locus_tag="ACHUU3_002369"
                     /gene_synonym="nagR"
     CDS             17839..19059
                     /gene="nagC"
                     /locus_tag="ACHUU3_002369"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR2082405.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            19107..19859
                     /gene="nagD"
                     /locus_tag="ACHUU3_002370"
     CDS             19107..19859
                     /gene="nagD"
                     /locus_tag="ACHUU3_002370"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFR2082406.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(19910..20041)
                     /locus_tag="ACHUU3_002371"
                     /pseudo
     CDS             complement(19910..20041)
                     /locus_tag="ACHUU3_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4910 a         5065 c         5033 g         5033 t
ORIGIN      
        1 tcttaacgca aatataaaac acgaaattac aacatttaag taacaaaaca gcatctaata
       61 tggcgatatt cataattaat gatttattat ctggggtatg atcgtttttt gttgatcttc
      121 ttcacagatt atagccattt catggataga ataactctac cttcaactga cacagcaaga
      181 ggtaaaggta aatggaaaat aataaccgct taatgcctca tataaggcgg acaacccata
      241 tcatgaagtt tgcccatcgt aatagttttg actttcattt ctttaatgcc cgctagtctt
      301 ctgactaaag ggcaccccaa cgtacaggtc tccctgactt taagcattac aggttaatac
      361 ctgtattcct cggtgctcat atactgctaa cccttttaaa ctctaaataa ttcgagtcgc
      421 agcacttgca acttgaggta tgacgagtat agccagttac cgggctggtc tgggttattg
      481 catctgcaaa aagcaaacta ctgatttatt tatcagcggt ggagctttgc tttttttccg
      541 gcgtgatcga tttctccttt gagaaattga ggacctgcta ttacctgaaa taaagagatg
      601 aataaaatgt cagaattaaa aattgccgtt agccgttctt gcccggattg tttttccact
      661 catcgtgcat gcgtgaatat tgacgaaagt aattatattg acgttgccgc cattatttta
      721 tcagtcaatg atgttgaacg tggaaaactc gatgaaatag acgctactgg ctatggcatt
      781 cctgttttta ttgcaacgga aaatgaagaa cgtgtaccag cagaatatct gccacgtatt
      841 tctggtgtct tcgagcattg cgaatcacgg aaagagtttt atggacgtca gttagaaacc
      901 gctgctagcc attatgaaac gcaactgcgc ccgcccttct tccgcgcact ggtcgattat
      961 gtcaatcagg gcaatagcgc ttttgactgc ccgggtcatc agggaggtga gtttttccgt
     1021 cgtcatccgg cagggaatca gttcgtggag tacttcggag aaacgctgtt ccgttccgac
     1081 ttatgcaacg cagacgtagc aatgggcgat ctgctgattc acgaaggcgc gccatgcatt
     1141 gcacagcaac atgcggcaaa agtgtttaat gccgataaaa catacttcgt tctaaatggc
     1201 acttcatctt ctaacaaagt ggttttaaac gccctgctaa caccgggtga tctggtgctg
     1261 tttgatcgca ataaccacaa atctaaccac cacggagcgt tgctacaggc tggtgcaaca
     1321 ccggtttatc tggaaacggc acgtaacccg tatggcttta tcggtggcat tgatgcgcac
     1381 tgttttgaag aaagttacct gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa
     1441 gaggctcgtc ctttccgcct cgctgtgatt cagttaggca cctacgacgg tacgatttat
     1501 aacgcccgcc aggtggtgga taaaattggt catctgtgtg actacattct gtttgactcc
     1561 gcatgggttg gctatgagca atttatcccg atgatggctg actgttcgcc gctgttgctg
     1621 gatcttaatg agaacgatcc gggtattctg gttacgcaat ccgtacataa acagcaggct
     1681 ggtttttctc aaacttcaca aatccataaa aaagacagcc acatcaaagg gcaacagcgt
     1741 tatgtaccgc acaaacgcat gaacaacgcc tttatgatgc acgcctccac cagcccgttc
     1801 tatccgctgt ttgccgcact ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt
     1861 aatatgtgga tggattgtgt ggtaaatggc attaatgccc gcaaactgat cctcgataac
     1921 tgtcagcata ttcgtccgtt cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat
     1981 gaaacagcgc aaattgcggt tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg
     2041 cattcttttg aaggctatgc agagaatcaa tactttgtcg atccttgcaa actgttgctg
     2101 acaaccccag gtattgatgc acgtaacggc gaatatgaag cgttcggtgt acccgcgacg
     2161 attcttgcta acttcctgcg cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc
     2221 atcctcttcc tgctgactcc ggcagaagat atggccaaac ttcagcaact tgttgccctg
     2281 ctggtacgct tcgaaaaact gcttgagtcc gacgcgccat tagcagaagt gctgccttcc
     2341 atctacaaac agcatgaaga gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa
     2401 atgcatgatt tgtatgcccg acacaacgtg aaacaactgc aaaaagagat gttccgcaaa
     2461 gcgcacttcc cacacgtcag catgaatccg caagaagcca actacgccta tttacgcggt
     2521 gaagtggagc tggttcgtct gccggatgcc gaaggtcgta tcgctgccga aggggcgctt
     2581 ccttatcctc cgggtgtgct gtgtgttgtt ccgggtgaaa tctggggtgg tgctgttttg
     2641 cgttacttca gcgctctgga agaagggatc aacctgctgc caggttttgc accggagctg
     2701 cagggtgtct atatcgaaga acatgatggt cgtaagcaag tttggtgcta tgtcatcaag
     2761 cctcgtgatg cgcaaagcac cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga
     2821 acaaaatggg cgtcgttcag ttaaccatac tgacgatggt caacatgatg ggctccggta
     2881 tcatcatgct gccgacaaag cttgccgaag ttgggacaat ttcaattatc tcctggctgg
     2941 tgacagccgt aggctcaatg gcactggcat gggcattcgc aaaatgcggt atgttcagcc
     3001 gtaaatcagg tggtatgggc ggttatgccg agtatgcttt cggtaaatcc ggtaacttta
     3061 tggcgaacta tacctacggc gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg
     3121 cggttggtta cggcaccgaa ctgctcggcg cgagtttgtc gccagtgcag attggtcttg
     3181 cgaccatcgg ggtgctgtgg atttgtaccg tggctaactt tggtggtgcg cgcattaccg
     3241 ggcaaatcag tagcattacc gtgtgggggg tcattattcc ggtggtaggc ctgtgcatta
     3301 tcggctggtt ctggtttagc ccgacgcttt acgttgattc ctggaatccg catcatgcac
     3361 cgttcttcag tgcagtaggt tcttccatcg ctatgacgct gtgggctttt ctcggtctgg
     3421 agtctgcgtg tgcgaatact gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg
     3481 tactcggcgg tacgttaggt gcggcggtga tttatatcgt ctccaccaac gtgattgcag
     3541 ggattgtgcc taatatggag ctggcaaatt caacggcacc atttggcctg gccttcgcac
     3601 agatgttcac accagaagtg ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg
     3661 gttcgctgct tggctggcag ttcaccattg cccaggtgtt taagtcttca tctgatgaag
     3721 gctacttccc taaaattttc tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt
     3781 tgaccattgt gattatccag agcgggttgg cgctgatgac gattagcccg tcgctaaaca
     3841 gtcagttcaa cgtgctggtt aacctggccg tggtgaccaa tatcattccg tatattctgt
     3901 caatggcggc attagtcatt attcagaagg tcgctaatgt gcccccctca aaagcgaaag
     3961 ttgcaaactt tgttgctttt gttggcgcga tgtatagctt ttatgcgctg tactcatccg
     4021 gggaagaagc catgctgtac ggttccatcg tgaccttcct tggttggaca ctgtatggtc
     4081 tggtatcacc acgctttgaa ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg
     4141 gcgatcttgc gaccgccctt tttttattaa atgtgtttac gcgtttttca gaacttcgct
     4201 aacaatctca accgcttctt tctcaatctg cttgcgatgt tcttcaccga ggaagctttc
     4261 gcagtagatc ttatatgcgt cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc
     4321 agtcatcact ttcagaccgc cgatagaagc accattgccc ggtgccgccg tcaggcgcgc
     4381 ggtgatcggg tcgcctgcca gggtgctggc gctcaccatt tccggagaca gcttagacag
     4441 cgccgctttt tgtgcggaag tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa
     4501 gcgttttgcc agttcgttgt agtgttcctg cgggttctta ccggtgacag cggtgatttc
     4561 cgccgccagc agacacatga tgatgccgtc tttgtcggtg gaccacggcg tgccgtcgaa
     4621 acgcaggaag gaagcccctg cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag
     4681 accatcaaca aaccatttga aacctaccgg gacttctacc agcttgcggc ccaagtcatt
     4741 gaccacacgg tcgatcatcg cagaagaaac cagcgtttta ccgacagcaa catctttgcc
     4801 ccactgcgga cgatgctgga acaggtagtt gatcgccacc gccaggtagt ggttcggatt
     4861 catcaaacct gccggagtga cgataccgtg acggtcataa tccgggtcgt tagcaaacgc
     4921 cagatcgaac ttatcacgca atgccagcag gcccgccatc gcacactcgg aggagcagtc
     4981 catacggatc gcgccgtctt tatcgaggtg cataaagcgg aaggtttgat cgacctgatc
     5041 gttaacgatg gtcaggttga ggttgtaata ctcgccaatt cgcttccagt attcgatacc
     5101 ggaaccaccc agcggatcaa cgcccagcgt caggcccgtt ttctgaatcg ccgccatatc
     5161 aacgatatcg gccagacctt ccacaaactg ctgcaccaga tcctgctctt tcacatgacc
     5221 ggatgccatc gcttcgtcga gggagatacg cttcacgcct ttcaggccat cggccatcag
     5281 tgcgttggca cgatcttcca ccactttagt gacgttggta tcagccgggc caccatttgg
     5341 cggattgtac ttgataccac catcttccgg cgggttatgg gagggtgtaa tcacgatacc
     5401 gtctgccagc gggccacctt ttttattatg aaccaggatg gcattggaaa cggcaggcgt
     5461 cggagtgaag ccattgtttt cctgcacaat gacatcaacg ccgttcgctg ccagcacttc
     5521 cagtacggaa atgaacgcag gctcggacag ggcgtgagta tctttaccca catagcaagg
     5581 gccagtgatg ccgtttttcg cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg
     5641 cggctcgtta aagctgtggc gcgctgcact gccacggtga ccggaagtac cgaatttcac
     5701 cgcgtgctcc gcattccctg cttctggttt cagtacataa tattgcgccg tcagttgggc
     5761 gacgttaatc aaatcactct gttgtgcagg ttggcctgca cgattgtgga ttgccattgc
     5821 tttgtccttt gtctgcaacg ttttagatag ttccgcaaac cttctcaatc aattccgccg
     5881 ggaattgcat cgactgcatg atgtgttcga tcatgctgca tttacggccg gtgttggtgt
     5941 tggtgatcac ccaatacggc gtgcctggca catgtttcgg cttggtctga ttaccatttt
     6001 tcagcagcgt ttgttcatct gccgcaaagt aaacgcgtgt acgaccgtgc aacgattccg
     6061 ttgcttcggc aaacgcctgg gcgtcaagag aatatagtgt agacaacagc agcataaagc
     6121 gattgaccgc tcgcttttgc tctgcgtatt cgtcagaaag cagaagttca cgcattgcgc
     6181 gaaccttgtc tttaatcgtt ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc
     6241 gaacctcttt cgtcaccgga gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc
     6301 gtaaaatgtc ggatgcgctc tcgccgatat gcttagtgtg gctggcaata tagctgtaga
     6361 gttcatcatc aacttcaatc gttttcatct taatccagta cagtgtctta gctgaataca
     6421 agtcgccaag agtatatgaa taaatcccgg cagcggatag cgtcaagcct ggcttgcaga
     6481 aaaactcgga ataaacctta tttgttgcag ccctgtggca caatggtcaa cataataccc
     6541 taacccgaca acgcgaaaaa aagaactttg ccatgaaatt gaatatccgc gcgcaaactg
     6601 cacaaaacca gcacaataat tctcccatcg ttcttgtcca tggtctgttt ggtagcctcg
     6661 acaaccttgg cgtactggct cgcgatctgg taaacgatca caatatcatc caggttgata
     6721 tgcgtaacca cggtctttca ccgagagatc cggtaatgaa ttacccggcg atggcgcagg
     6781 atcttgttga tactctggat gcacagcaga tcgacaaagc aacatttatc ggtcactcca
     6841 tgggcggtaa agcggtaatg gcacttacgg cactggcccg cgatcgcatc gataaactgg
     6901 tggcgatcga tatcgcaccg gtcgactatc acgtacgccg tcatgatgag attttcgcgg
     6961 ctatcaacgc ggtcagtgaa tcggacgcac aaactcgcca gcaagcagca gcaataatgc
     7021 accagcatct taatgaagaa ggggtgattc agtttctgtt gaaatctttt gttgacgggg
     7081 agtggcgctt taacgtgccg gtattgtggg atcagtatcc gcatattgtg ggttgggaga
     7141 aaatcccggc atgggatcac cctgccctgt ttatccctgg cggcaattct ccgtatgtta
     7201 gcgagcagta ccgtgatgat ttactggcac aatttccaca ggcacgggcg catgtgattg
     7261 caggcgcggg tcactgggtc catgctgaaa aaccggatgc ggtattacgc gctatccgtc
     7321 gctatctcaa tgattaacgc aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg
     7381 tgggcgttgg cgtgactcat cgcgctgatg tattatggcg cgctatccat ccgggcagag
     7441 gcctggctga ttgtttcccc cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt
     7501 acgacattag atctgttcgc gcacgagcgt cgaccgggac gaccgaaaac taatccgctt
     7561 tcgcgcgatg aacagctgcg tattaataaa cgcaaccagc taaaacgcga caaagtacgt
     7621 ggccttaagc gtgtcgaact gaagctgaac gcggaagctg tcgaggcgct gaacgagctg
     7681 gcggagtcgc gcaatatgag ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg
     7741 gcggcgctgc gtagtcaggg catcgtttaa atttccactt tcatgtagca cagtgtgcag
     7801 tcctgctcgt ttgctgattc cgcaagactg cctgttctgc tatgattgcc tttatccgtg
     7861 ggcaattttc cacccccatt tcaataagtt tcaagaggtt atttcactca tggctatcac
     7921 tggcatcttt ttcggcagcg acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa
     7981 acagcttggt aaagacgttg ccgatgtcca tgacattgca aaaagcagca aagaagatct
     8041 ggaagcttat gacattctgc tgctgggcat cccaacctgg tattatggcg aagcgcagtg
     8101 tgactgggat gacttcttcc cgactctcga agagattgat ttcaacggca aactggttgc
     8161 gctgtttggt tgtggtgacc aggaagatta cgccgaatat ttctgcgacg cattgggcac
     8221 catccgcgac atcattgaac cgcgcggtgc aacgatcgtt ggtcactggc caactgcggg
     8281 ctatcatttc gaagcatcaa aaggtctggc tgatgacgac cactttgtcg gtctggctat
     8341 cgacgaagac cgtcagccgg aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat
     8401 ttctgaagag ttgcatctcg acgaaattct caatgcctga tgtgatgcgg cgtagactca
     8461 tgtctacgcc gtattaatag ataatgccaa tcaaaataat tgctacaaat ttgtaacttt
     8521 tgctgttgta cctgtacaat gtcccggtgt tcaagtggcc ttgccgttgt aaatgtaagc
     8581 tgtgccacgt ttttattaac aatatttgcc agggacttgt ggttttcatt taggcgtggc
     8641 aattctataa tgatacgcat tatctcaaga gcaaattctg tcacttcttc taatgaagtg
     8701 aaccgcttag taacaggaca gattccgcat gactgataac aataccgccc taaagaaagc
     8761 tggcctgaaa gtaacgcttc ctcgtttaaa aatcctagaa gttcttcagg agccggacaa
     8821 ccatcacgtc agtgcggaag atttatacaa acgtctgatc gatatgggtg aagaaattgg
     8881 tctggctacg gtatatcgcg tactgaacca gtttgacgac gctggtatcg tcacccgcca
     8941 caattttgaa ggcggtaaat ccgtatttga actgacacag caacatcacc acgatcacct
     9001 gatctgcctc gactgcggca aggttatcga atttagtgat gattccatcg aagcgcgtca
     9061 gcgtgaaatt gccgcaaaac atggcattcg cctgactaac cacagtctct atctttacgg
     9121 tcactgtgcc gaaggcgatt gccgcgaaga tgaacatgcg cacgaaggca aataagccag
     9181 cctgaatgag aaaaagccaa cctgcgggtt ggctttttta tgcaagggaa ataagaaaag
     9241 cgagagttac agctctcact tatttgttat tactgcgaat ttcctgccaa actttatcac
     9301 aatcggcttt caccgcctga tcatttccgg tttgcgttgc acgcagacac tgctgataat
     9361 ccagtacgcg gacactttcc tggtcggcaa aatgctgatg ttgtttctct ttcttcagca
     9421 cgtttaacac gctctggcag gcttcaattt tttctggcga accttgtgcg gtgttgatac
     9481 aggcgctgta tgcctctttc agacgactgt cttcctgtgg cgcggttgat tgcgcacaag
     9541 ctaccagccc cgatgccatt atggcgatga gaatcagttt tttcatagca aattcctcaa
     9601 acgtgcggca ggcacaaaac ctgccgcgtc gggcatcaga agatggtgaa tggtgcgatt
     9661 accataaatt tcacgtcacg ctcatcctgg aagatgttgc cgtaaccacc gccccagctt
     9721 gggatgtcgg agtggttgtc gtattcggtg aagtgcagtt tgaacatcgt gcctttggcg
     9781 cgaccgtcct gaatggtgta gaccgcatcc aggctgtatg cagactcttc aatagtccgg
     9841 tttttgtcgt agtacgcatc cggattgctc tgctaggtcg caggtttagc atcccatgcg
     9901 taaacgtagg aagcgccgat ggcgaagcct ggaagattcc agtttttcag gtcatacatc
     9961 gcaccgaaga agaccgcttt ttcgccgtta gcgttgaagt cggaacggtt atcccaccag
    10021 atatccaggc ggccgttgga ggaagcgtag gttggagtca tacgttgcag gaagtatccc
    10081 tgctgaccgt cagccttaac ccaggtgcct tcgaggcgca aatccactac gtcagccgcc
    10141 cggtaaccaa aggtcaacgc ttgcagccag gcggtgccgt cataaaggtc gttgacgctg
    10201 cgatcgtcaa ctttatcgcg cgtaccatag aactggtagc tggtggttaa cgggctaccg
    10261 gcgatatcaa atttgtagct ggctttggca aaatattgat cgatataccc ttccgcctga
    10321 ccaaatgccg cttccagtac gaagttattt ttgaagtcgt atttcgcccc aagggagtgc
    10381 agataatcaa ctttggtggt tttatcgttc tgataaaact catccatttc cagatgccac
    10441 ggcgctttgt attcgttggt ccacatgtag gagaaactca acgcaccagc atcgccgtaa
    10501 tcaaaattcg ccccggcttc cgccccctga taagtacctg gcataaagct ccagtgcggc
    10561 gctaacagcg tttgaccagt tggctgaatg taacctgccc tcgcccaaac cggaccgtat
    10621 ttaaatttgg ccgcagcttt atacaggctt ataccgcttt tgtcgccgga ccagtcttcg
    10681 tcataggctt tattactttt tgaaaacgcg atttcgttcg ggtgggagct gtcgccgttt
    10741 tccgccattt caatcgccgt aaacgcagca atatcaaggc cgaacatatc agcagcataa
    10801 ccggactgaa aatcgaggtt ggcattccag gtggagtgag aaaggttggt tttgtatttg
    10861 tcgccgtcgg taacatcttt acggtcgcgt tcacgctgcc agtaatatat accgccggtt
    10921 aaggttgaat cgtcgatgaa tccttcggcg cgagcctccg gcattgccat aaagcccgac
    10981 attgctgtaa caccggcgat agccagcgcc agcgtactat gtttgccact aaacgtacgc
    11041 atgggttaat cctctttgac gtataaattg ctgcaccaaa ggtgaacagc gaaaaaataa
    11101 aaattaaaaa agtagcgttg agaaaagtct cgctgactac aggaacagac tatttttcgc
    11161 gacgcgaatt atcaatctct ttctgtaacc agaatgtaag aacatgagca accgcacata
    11221 ttttgatgat ttttgttaca aggcttaagt ttgcggttga aaggcttgca gaagaataaa
    11281 agcgtgaaat tccagcaaga tgaaagcggt tacattttat ttaattatgt tttgttattt
    11341 taaatttatt ttatggataa attgcgaagc ggaattaatt cgcgaagaaa agcagaaaaa
    11401 acgccgctga acgcggcgtt ttggattgtt tgcatatttc ttgccggatg cggcgtgaac
    11461 gccttaaccg gcctacaaaa gcacgcaaac tcaatatatt gcagagatca tgtaggcctg
    11521 ataagcgtag tgcatcaggc aatttagcgt ttgaaactgt ttgtctgttc cttgccggat
    11581 gcgacgtaaa cgccccatcc ggcatagcga aacttaaaat tactcgccta ctttcgccca
    11641 ggtatcacgc agcccaacgg tgcggttaaa taccggtttt tccgccgtag aatggcggct
    11701 atcgaggcag aagtaacctt cacgctcaaa ctggaatgct ttacccgcaa ccgcatcttt
    11761 cagcgacggt tcagcaaagc cctgtttgat caccagcgat tccgggttaa tcaccgacag
    11821 gaaatcatcc gcagcaccag ggttcggcac gctgaacaga cggtcgtaca gacggatttc
    11881 aaccggcagc gcatgtgccg cgctcaccca gtgaataaca cctttgactt tacgaccatc
    11941 tgccggatct ttgcttaagg tgtcggcgtc ataagtacag aagatggtgg tgatattgcc
    12001 ttcggcatct ttctccacgc gttctgcctt aatgacgtaa gcattacgca gacgcacttc
    12061 tttacccagc accagacgtt tgtactgctt gttagcttct tcgcggaaat cggcgcgatc
    12121 gatccaaatc tcaccgctaa acggcacctg gcggctaccc atttccggtt tgttcggatg
    12181 gttcggcatg gtaaccattt cgccttcgcc ctgatagttt tcgataacca gtttcaccgg
    12241 atcgataacc gccattgcgc gcggggcgtt ttcgttgaga tcttcacgga tgcaggattc
    12301 cagcgacgcc atctcaatgg tgttgtcctg cttggtcacg ccgatgcgtt tgcagaactc
    12361 acgaatagaa gcagcagtgt aaccacgacg acgcagaccg gaaatggtcg gcatacgcgg
    12421 gtcatcccag ccttcaacgt gcttgtcggt caccagcagg ttcagcttac gcttggacat
    12481 cacagtgtat tccagattca ggcgcgagaa ttcgtactgg cgcgggtgaa caggaatggt
    12541 gatgttgtcc agcacccagt cgtacaggcg acggttgtcc tggaactcaa gcgtacacag
    12601 agagtgcgta ataccttcca gggcatcgct gatgcagtgg gtgaagtcgt acatcgggta
    12661 gatgcaccac ttgttgccag tctggtggtg ttcagcaaat ttaatgcggt acagcaccgg
    12721 atcgcgcatc acgataaacg gtgaagccat gtcgattttc gcacgcaggc aggctttacc
    12781 ttcttcaaaa ccaccggcac gcattttttc gaacagcgcc aggttctctt caacgctgcg
    12841 gtcgcggtac gggctgtttt tacccggttg cgtcagggtg ccgcggtatt cgcggatctg
    12901 ttccggcgtc agttcatcaa cgtacgccag gcctttattg atcagttcga tcgcataggc
    12961 gtggagctga tcaaaataat cggaggagta acggacgtta ccagaccagt gaaaacctaa
    13021 ccactctacg tcgtttttga tcgactcaac atactcgata tcttctttta ccgggttagt
    13081 gtcgtcgaaa cgcaggttgc actggccttt atagtcctgg gcgatcccga agttcaggca
    13141 gatagatttc gcatggccaa tatgcagata gccattcggc tccggcggga aacgggtgtg
    13201 tactgtggtg tgcttaccac tggccagatc ttcatcgatg atctgacgga taaagttagt
    13261 cgggcgggct tctgcctcac tcatcgtgga ttcctcaaag cgtaaacaac gtataacggc
    13321 gtatgatctt ataagcggga caggctgaca actgttagtt agcgaaaatc gaaaatctca
    13381 ctgagattat ggggatgatt gatgaaaaaa aaagcggcag agaagatctc caccgctttt
    13441 gccagtcccg cgtcattgtt ggatgcgcgt cttatccaac caggactgag cttttgtagg
    13501 cctgataaga cgcgtcaagc gtcgcatcag gcataaagca gattactttt tgatttcata
    13561 cagcggtgtt tgacccgcca caacattgcc ctgagcttta atgatcaagc cactgaaatc
    13621 gtcgatattg ctgcaaacca ccgggctaat catcgaacgg gcgttagcgt tcaggtaatc
    13681 cagatccatt tccagaatcg gttgccctgc gctcacctgc gcgccctctt ccaccagacg
    13741 tttaaagcct ttaccttcca gcgctacggt gtcgataccc atgtggacga caatctccgc
    13801 gcctttttcg gtttctaggc agaacgcgtg gttggtgttg aagattttca cgattgtccc
    13861 tgcggctggt gatacgacga ttttatctgt cggtttcacc gccacaccgt cacccaccgc
    13921 tttgctggcg aatgcttcgt cagaaacctg atccagtgcc acgacatcac cggtaatcgg
    13981 cgataccagc tccgcgatag ataccgcgtt tggtacagcc tgcggttttg ctacaggagc
    14041 ggcagttgcc ggagttgctt cagctgacgc agctgctacc gggccacggg caacgacttt
    14101 cttcatcgca tcgccaatgg attctgcttt cgcgccaaca atcacctgaa tggtctgttt
    14161 gttcagtttt actacaccag aagcacccag acgtttacac atcgcatcgt tgacgcgagc
    14221 tgagtcagcc acggtaaggc gcagacgggt aatacaggcg tcaatcgctt tcaggttgtc
    14281 agtaccgcca accgcagcaa tatagttggt tgccagttga gtcagacctt cttcagtatt
    14341 gctgttagcc tcttcagtta cgatctcgtc ttctttatct tcacgacccg gcgttttcag
    14401 gttgaacatg cggataacca aactgaacac cacgaagtag atagcgaaga agacaacacc
    14461 catcaccagc agcatccaga cgttctggct ggcggccggc aggttataca tcaacgcgta
    14521 gtcgatagcc cccgcagaga aagagaagcc agcatggata cccagcagcg ttgccacaaa
    14581 caggctgata ccggtcagca gtgcgtgcag gaggtacagc agcggagcaa ggaacatgaa
    14641 caggaattcc agcggctcag tcacaccggt caggaacgca gtaacagcaa cagaaagcag
    14701 catcccgcca accatcggac gacgctcttt cggtgctgcg aagtacatcg ccagcgccgc
    14761 acccggcaga ccgaacatca tgatcgggaa gaagccggac atgaacatcc ccgcggtgcc
    14821 gtcaccagcg tagaaacggt tgatgtcgcc gtggaaaacc gtacccgccg cgttggtgaa
    14881 ttcaccaatc tggaaccagg cgatggtgtt cagcacctga tgcagaccgg ttgggatcag
    14941 cagacggttg atgaaaccaa agataccgga acccagcgcg cctgcagaaa cgatccactc
    15001 gccgcctgca tggatagcgt gctgtactgg cggccagacg taaccaaaaa tggccgccag
    15061 caccaggcag aagaagccgg tggcaatcgg cacaaagcgt ttgccgccga agaagctcag
    15121 gaagtccggc agtttaatat cggaccaacg gttataggct gcgccaccaa ccagaccggt
    15181 aatgataccc gccagtacac ccatgttaat ttctgggttg atggtcacca tcgctttggt
    15241 taacacaaag taacctaccg cacccgccag cgccgccgca cctgcgctgt ctttcgacca
    15301 gctggatgcc acaccgatgg cgaagattaa tgcgaggtta tcaaaaatcg caccgcccgc
    15361 ctgggcaata aacgcaacgt taagtaaatc tggctgaccg aatcgcagca acagtgccgc
    15421 caccggcagc accgcgatag ggagctgtaa cgccctaccg agtcgctgga aaaaacctaa
    15481 aatattcatc ttattccccc tacgagaacc ctatttggct cgtttaaagc cgtattttta
    15541 ttttgctgca aattgtactg ccgatgttct gtaatcagat tgttagatca tctgctacag
    15601 agtgtgtgaa aatttaattc gtatcgcaaa ttaaacgcgt gccttttgtg agttttgtca
    15661 ccaaatatcg ttatcatcac tcccttttac tggctaaagc agaaaactta ttttatcatt
    15721 caaaaaatca ggtcggattg acgcctgtct gcgcaaatcc aggttacgct taaagatgcc
    15781 taatccgcca acggcttaca ttttacttat tgaggtgaat aatgagactg atccccctaa
    15841 ctaccgctga acaggtcggc aaatgggctg ctcgccatat cgtcaatcgt atcaatgcgt
    15901 tcaaaccgac tgccgatcgt ccgtttgttc tgggcctgcc gactggcggc acgccgatga
    15961 ccacctataa agcgttagtc gaaatgcata aagcaggcca ggtcagcttt aagcacgttg
    16021 tcaccttcaa catggacgaa tatgtcggtc tgccgaaaga gcatccggaa agctactaca
    16081 gctttatgca ccgtaatttc ttcgatcacg ttgatattcc agcagaaaac atcaaccttc
    16141 tcaacggcaa cgccccggat atcgacgccg aatgccgcca gtatgaagaa aaaatccgtt
    16201 cttacggaaa aattcatctg tttatgggcg gtgtaggtaa cgacggtcat attgcattta
    16261 acgaaccggc gtcttctctg gcttctcgta ctcgtatcaa aaccctgact catgacactc
    16321 gcgtcgcgaa ctctcgtttc tttgataacg atgtcaatca ggtgccaaaa tatgccctga
    16381 ctgtcggtgt tggtacattg ctggatgccg aagaagtgat gattctggtg ctgggtagcc
    16441 agaaagcact ggcactgcag gctgcagttg aaggttgcgt gaaccacatg tggaccatca
    16501 gctgtctgca actgcatcca aaagcgatca tggtgtgcga tgaaccttcc accatggagc
    16561 tgaaagttaa gactttaaga tatttcaatg aattagaagc agaaaatatc aaaggtctgt
    16621 aattgttatc cctgccctgg ctccttgctc agggcaatat tttttaaaat cggggggcag
    16681 aatgtatgca ttaacccagg gccggatctt taccggccac gaatttcttg atgaccacgc
    16741 ggttgttatc gctgatggcc tgattaaaag cgtctgtccg gtagcggaac tgccgccaga
    16801 gatcgaacaa cgttcactga acggggccat tctctccccc ggttttatcg atgtgcagtt
    16861 aaacggctgc ggcggcgtac agtttaacga caccgctgaa gcggtcagcg tggaaacgct
    16921 ggaaatcatg cagaaagcca atgagaaatc aggctgtact aactatctgc cgacgcttat
    16981 caccaccagc gatgagctga tgaaacaggg cgtgcgcgtt atgcgcgagt acctggcaag
    17041 acatccgaat caggcgttag gtctgcatct ggaaggtccg tggctgaatc tggtaaaaaa
    17101 aggcacccat aatccgaatt ttgtgcgtaa gcctgatgcc gcgctggtcg atttcctgtg
    17161 tgaaaacgcc gacgtcatta ccaaagtgac cctggcaccg gaaatggttc ctgcggaagt
    17221 catcagcaaa ctggcaaatg ccgggattgt ggtttctgcc ggtcactcca acgcgacgtt
    17281 gaaagaagcg aaagccggtt tccgcgcggg gattaccttt gccacccatc tgtacaacgc
    17341 gatgccgtat attaccggtc gtgaaccggg cctggcgggc gcgatcctcg acgaagctga
    17401 catttattgc ggtattatcg ctgatggcct gcatgttgat tacgccaaca ttcgtaacgc
    17461 taaacgcctt aaaggcgaca aactgtgtct ggttaccgac gccaccgcgc cagcaggtgc
    17521 caacattgaa cagttcattt ttgcgggtaa aacaatatac taccgtaacg gactttgtgt
    17581 ggatgagaac ggtacgttaa gcggttcatc cttaaccatg attgaaggcg tgcgtaatct
    17641 ggtcgaacat tgcggtatcg cactggatga agtgctgcgt atggcgacgc tctatccggc
    17701 gcgtgcgatt ggcgttgaga aacgtctcgg cacactcgcc gcaggtaaag tagccaacct
    17761 gacctcattc acacctgatt ttaaaatcac caagaccatc gttaacggta acgaggtcgt
    17821 aactcaataa gagaaagtat gacaccaggc ggacaagctc agataggtaa tgttgatctc
    17881 gtaaaacagc ttaacagcgc ggcggtttat cgcctgattg accagtacgg gccaatctcg
    17941 cggattcaga ttgccgagca aagccagctt gcccccgcca gcgtaaccaa aattacgcgt
    18001 cagcttatcg aacgcgggct gatcaaagaa gttgatcagc aggcctccac cgggggccgc
    18061 cgcgctatct ccatcgtcac cgaaacccgc aatttccacg caatcggcgt acggcttggt
    18121 cgtcacgacg ccaccatcac cctgtttgat ctcagcagca aagtgctggc agaagaacat
    18181 tacccgctgc cggaacgtac ccagcaaacg ctggaacatg ccctgttgaa tgccattgct
    18241 cagtttattg atagctacca gcgcaaactg cgcgagctga tcgcgatttc ggtgatcctg
    18301 ccagggcttg ttgacccgga cagcggcaaa attcattaca tgccgcatat tcaggtagaa
    18361 aactgggggc tggtagaagc tctggaagaa cgttttaaag tgacctgttt cgttggtcac
    18421 gatatccgta gtctggcgct ggcggagcac tacttcggtg caagtcagga ttgcgaagac
    18481 tccattctgg tgcgtgtcca tcgcggaacc ggggccggga ttatctctaa cgggcgcatt
    18541 tttattggcc gcaacggtaa cgtcggtgaa attggccata ttcaggtcga accgctgggt
    18601 gaacgctgcc actgcggcaa ctttggctgc ctggaaacta tcgccgccaa cgctgccatt
    18661 gaacaacggg tgttgaatct gttaaagcag ggctaccaga gccgcgtgcc gctggacgac
    18721 tgcaccatca aaactatctg caaagccgcg aacaaaggcg atagtctggc gtcggaagta
    18781 attgagtatg tcggtcgtca tctgggtaaa accatcgcca ttgctatcaa cttatttaat
    18841 ccgcaaaaaa ttgttattgc cggtgaaatc accgaagccg ataaagtgct gctccctgct
    18901 attgaaagct gcattaatac ccaggcgctg aaggcgtttc gtactaatct gccggtggta
    18961 cgttctgagc tggatcaccg ctcggcaatc ggcgcttttg cgctggtaaa acgcgccatg
    19021 ctcaacggta ttttgctcca gcatttgctg gaaaattaat gtgcttttat agtggcgctt
    19081 attgttgtca atattctggg tagtccatga ccattaaaaa tgtaatttgc gatatcgacg
    19141 gcgtgctgat gcacgataac gtcgccgtac cgggtgcagc ggaatttttg cacgggatta
    19201 tggataaagg cctgccgctg gtgttgctga ccaactatcc ttcgcagacc gggcaagatc
    19261 tggcgaaccg ctttgccact gccggtgtcg atgtgccgga tagcgtgttt tatacctctg
    19321 cgatggcgac cgccgatttc ctgcgtcgcc aggaaggcaa gaaagcatat gtggtgggcg
    19381 aaggcgcact gattcatgaa ctgtacaaag ccggtttcac tattaccgat gtgaaccctg
    19441 atttcgtgat tgttggcgaa acgcgttcct acaactggga catgatgcat aaagcggcct
    19501 atttcgtcgc taacggtgca cgctttatcg ccaccaaccc ggacacccac gggcgcggtt
    19561 tttatcccgc ttgtggcgcg ttgtgtgcag ggattgagaa aatctccggg cgcaaaccgt
    19621 tctatgttgg taagcccagc ccgtggatca tccgcgcagc attaaacaaa atgcaggcgc
    19681 attcggaaga aacggtgatt gtcggcgata acttgcgcac cgacattctg gcgggcttcc
    19741 aggcaggtct ggaaacgatt ctggtgcttt ctggtgtttc atcgctcgac gatatcgaca
    19801 gtatgccgtt ccgcccaagc tggatttacc cgtcagtcgc tgaaatcgac gttatctgaa
    19861 taccacccac gtctgcggac gtggttggct ctgatgacaa acgccaaact gcctgatgcg
    19921 ctacgcttat caggcctacg cagctcctgc aatatattga attttcatgc ttttgtaggc
    19981 cggataaggc gttcacgccg catccggcat gaacaaatcg cactttatca gcaatttggc
    20041 c
//
LOCUS       DAZPLI010000070        19908 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZPLI010000070 DAZPLI010000000
VERSION     DAZPLI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.071"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            112..171
                     /gene="ibsE"
                     /locus_tag="ACHUU3_002372"
     CDS             112..171
                     /gene="ibsE"
                     /locus_tag="ACHUU3_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFR2082407.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(291..1724)
                     /gene="hldE"
                     /locus_tag="ACHUU3_002373"
     CDS             complement(291..1724)
                     /gene="hldE"
                     /locus_tag="ACHUU3_002373"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR2082408.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1772..4612)
                     /gene="glnE"
                     /locus_tag="ACHUU3_002374"
     CDS             complement(1772..4612)
                     /gene="glnE"
                     /locus_tag="ACHUU3_002374"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR2082409.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(4635..5936)
                     /gene="ygiF"
                     /locus_tag="ACHUU3_002375"
     CDS             complement(4635..5936)
                     /gene="ygiF"
                     /locus_tag="ACHUU3_002375"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR2082410.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6178..6798
                     /locus_tag="ACHUU3_002376"
     CDS             6178..6798
                     /locus_tag="ACHUU3_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR2082411.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6862..8100
                     /gene="cca"
                     /locus_tag="ACHUU3_002377"
     CDS             6862..8100
                     /gene="cca"
                     /locus_tag="ACHUU3_002377"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR2082412.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8263..9084)
                     /gene="bacA"
                     /locus_tag="ACHUU3_002378"
     CDS             complement(8263..9084)
                     /gene="bacA"
                     /locus_tag="ACHUU3_002378"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR2082413.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9175..9543)
                     /gene="folB"
                     /locus_tag="ACHUU3_002379"
     CDS             complement(9175..9543)
                     /gene="folB"
                     /locus_tag="ACHUU3_002379"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR2082414.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            9648..10265
                     /gene="plsY"
                     /locus_tag="ACHUU3_002380"
     CDS             9648..10265
                     /gene="plsY"
                     /locus_tag="ACHUU3_002380"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR2082415.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10278..11210)
                     /gene="ttdR"
                     /locus_tag="ACHUU3_002381"
                     /gene_synonym="dan"
     CDS             complement(10278..11210)
                     /gene="ttdR"
                     /locus_tag="ACHUU3_002381"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFR2082416.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            11417..12328
                     /gene="ttdA"
                     /locus_tag="ACHUU3_002382"
     CDS             11417..12328
                     /gene="ttdA"
                     /locus_tag="ACHUU3_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFR2082417.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            12325..12930
                     /gene="ttdB"
                     /locus_tag="ACHUU3_002383"
     CDS             12325..12930
                     /gene="ttdB"
                     /locus_tag="ACHUU3_002383"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR2082418.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12979..14442
                     /gene="ttdT"
                     /locus_tag="ACHUU3_002384"
     CDS             12979..14442
                     /gene="ttdT"
                     /locus_tag="ACHUU3_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR2082419.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14485..15498)
                     /gene="tsaD"
                     /locus_tag="ACHUU3_002385"
     CDS             complement(14485..15498)
                     /gene="tsaD"
                     /locus_tag="ACHUU3_002385"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR2082420.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15735..15950
                     /gene="rpsU"
                     /locus_tag="ACHUU3_002386"
     CDS             15735..15950
                     /gene="rpsU"
                     /locus_tag="ACHUU3_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR2082421.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            16061..17806
                     /gene="dnaG"
                     /locus_tag="ACHUU3_002387"
     CDS             16061..17806
                     /gene="dnaG"
                     /locus_tag="ACHUU3_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR2082422.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            18001..19842
                     /gene="rpoD"
                     /locus_tag="ACHUU3_002388"
     CDS             18001..19842
                     /gene="rpoD"
                     /locus_tag="ACHUU3_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR2082423.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4711 a         5560 c         5130 g         4507 t
ORIGIN      
        1 tcagggaaag cgttgcgcgg ggtccggcgc tcatcaacaa tcggggagtc agcaaggggc
       61 tgaaacggga aagcccctcc cgaggaaggg gccttataga aggaaagggt tatgatgaag
      121 ttcgtcatca tactggttgt gttgttactg ttaagtttcc cgacttacta acaactgatc
      181 agagggggaa gaaatcctcc cttacccttg ttcctttact ttaggttgaa aaaacaacag
      241 cgtcaatagg cctgcctgct acaaagcgag atctgtgaac cgctttccgg ttagcctttt
      301 ttatcctgtt ggatcttctt gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc
      361 accagcactt cgccgccgtt ggcccagact tctttactcc cggcgatctc ttctggttta
      421 tagtcgccgc ccttcaccag taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg
      481 tcttcttcaa acgacaccac ccagtcgacc gcttccagtg cgcccagcac aatcatacgc
      541 tgttcgagcg ggttaacggg gcgggaatcc cctttcagcc gtttggtgga ggcatcgctg
      601 ttcacagcga caatcagacg gtcacccagt ttgcgagcat ttgccagata ggagacgtgc
      661 ccggcgtgca ggatgtcaaa gacaccgttg gtcattacca ccttttcgcc gcgtttacgc
      721 gctgccgcta cggccagctt cagttcctct tcggtcatca cgccaaagcc ggtatctgcg
      781 cgcccacgca ctgcattttc cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg
      841 accaccacgc cagccgccgc attggcaaag aagcaggctt cttccagcga attacccgct
      901 gccagcgttg ccgccaggac gccaatcacc gtgtcgcccg caccggtaac gtcatacact
      961 tcctgcgcct gggttggcat atgcagcggc gctttacccg gttgcagcag cgacataccc
     1021 tgttcggaac gggtcactaa cagagccgag agttcgtaat cggcaatcag tttcatgccg
     1081 cgctcaacaa tctcttcttc ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga
     1141 ttcggcgtta acagcgtagc gccgcggtag cgctcaaaat cggtaccttt tggatcaatc
     1201 agcaccggaa cgcccgcttt acgcgccagt tggatcatct gctgtacgct tgccagcgca
     1261 cctttggcgt agtcagaaag caccagcgcg ccaatcgaac tcagcgcctg attaatccgc
     1321 tcgtgcagcg gctgcggatc aacgccttcg aaaccttctt cgaaatccag acggatcagc
     1381 tgttggttgc gggaaagtac ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg
     1441 aagtcgcatt tgacgttgac gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca
     1501 atgcccgtca acccgaccag gcgtgcatta gcaccgagag aagcgatatt catcgccacg
     1561 ttagccgcgc cgcccggacg ttcttcgatg gtattcactt taaccacggg caccggcgct
     1621 tccggcgaga tacggctgga gggaccgtac cagtaacgat ccagcattac atcaccaacc
     1681 accatcactc ctgcacgttc aaactctggc agcgttactt tcattcctgt ctcctgagag
     1741 attcaaaatt tgcgcgcgat aataccatac ttcattcttc caccagccac ttctgccagc
     1801 ttgcccgcac cagatcacgc tctgcggtga agcaatcccc cgacacatgg cccggcaatt
     1861 cctgtaatgc cagatgatga agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca
     1921 tcgcttcctg ctcttccata atgtcgtttt gcgccagcag ctccagaata cgcacgttgt
     1981 ctgaccagcg cgttaacttc ggtttttcat gagcgtagcg caacaccaga tattgggtaa
     2041 taaattcgat atcggtaatt ccgccttcat cagctttgat atcaaagcga tcgcgatgtt
     2101 tattgccgag atgagcgcgc attttctcgc gcatttcccg cacttccgtt tgcagagttc
     2161 taccttcacg cggcagcgtc ataatctcgc gacgcactgc gtcaaagtgc gcggtgagct
     2221 gcggatcgcc gtacactacc cgcgcacgca ccagcgcctg atgttcccac gtccaggcct
     2281 cgtttttctg ataatcggca aatgcttctg tggaggtaac cagcattccc gccgccccgg
     2341 acgggcgcag ccgcgcatcc acttcgtaca aaatgccgga agaggttcgc gtgctgaaca
     2401 ggtgcataat gcgttgcgcc agacgaagat aaaactgtcg tccgtcgatt tcccgctcgc
     2461 cgtcagtcat cgcatccatt gggcaatcat ggaggaagat caggtcaaga tcggaactgt
     2521 agcctaactc ccagccgccc agcttgccgt agccgaccac tgcaaaacca cgcccttcgc
     2581 gatcgttgag gtgattcggc ttaccgtagc gggcaaccat ttgaacccac gcctgctgaa
     2641 cgacggcatc tatcatggct tccgccagcc aggttaagtg atcgctcact ttcatcaccg
     2701 gtagcgtacc agcgatatcc gctgcggcga tgcgtaacag ctgcgcctgt ttgaactgac
     2761 gcagcgcctc aagctgttgc tcttcgtcat cttccggcac gcgcagcaaa tactggcgca
     2821 actcatcgcg gtaggcatcg gtcgccgtcg gctggtaaag agtgtttgga tcgagcaatt
     2881 catccagcaa taatggataa cgcgccagct ggctggcaat catcggcgat gcggcacaca
     2941 gagaaatcaa atggttaagc gccgcaggga attcactgag caattctaaa taggtggtgc
     3001 gggtaacaat ccccaccagc aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac
     3061 gcgcacagac atcacttagc agatgcggca tcagatggtc gagcacctga cgtcctcgcg
     3121 gcccgatggt gcgcttatcc agctctttgc ggaaatcgga aatcagcgtt agcacctgtt
     3181 tgcgatcatc ctcgctaaga tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat
     3241 cctgccacag ctcacgccac tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt
     3301 cgccaatcaa ttcattaaac acccggcgca cattgttcat atgtgcggtc agcgccccgg
     3361 tcagttgcgg ccagtcagca aagtccatcg cccacgccag ccgcgcacgg ttaagctcat
     3421 cagaaggaag cgtctgggtt tgttcgtcgt taatgctttg cagcaggttt tccagacgcc
     3481 gcaggaacag atacgccact cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg
     3541 cagcaatggc gctgagcgtt ggcagtaaag agcacgattg cagcgacggt tcgcgcccgc
     3601 cgcgaatgag ctggaatacc tggacgataa attcaatttc gcgaatacca cccgcgccaa
     3661 gtttgatgtt gtcggtcaaa ccgcgccgac gcacttcacg ggcaatcatt cctttcatgt
     3721 tgcgcagcga ctgaatcacg ctgaaatcga tgtaacgacg gaaaacaaac gggcgtagca
     3781 tcgcacgcaa ctcgttagca tagacgcctt cgctatcgcc cataatccgc gctttgacca
     3841 tcgcgtaacg ctcccagtcg cgcccctgct cctggtaata atcctccaac gcggcaaagc
     3901 tcagcaccag cgggccactt tcgccaaacg gacgcagtcg catatccacg cgatagacga
     3961 agccatcctg cgttggttga tccagcactt taatcagccg ctgccccatg cgggtaaaaa
     4021 actgcgcgtt atccagttcc cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa
     4081 aaatcagatc gatatcagag gagaaattca gttccccgcc gcccagctta cccatgccta
     4141 agatcagcag cggttgcgct tcgccctgcg cattgcacgg cgttccccac tcgcggcagc
     4201 aggcgtcata cagccagtca cgcgccgcaa caatcagcgt ctccgccaga taactgagct
     4261 gctgcaatat gctctcttca gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca
     4321 taatgcgccg ccggaatagc cgcagctcgc gcattaaccc ggcttcgtca ctcacattac
     4381 tgagcgcctc ctgcaaccat gcagcgtaat gctgccattc atcggcctgc ggcggttggc
     4441 tttccagttc cgtcaaccac tctcgatgcg caattatgct gtcctgcaca aaatcactga
     4501 aagtaagtac tgacttcgcc tgtgcactta gtggttcctc ggctaaaggc tctggcagcc
     4561 gctcaacaac ggtctgccag tactgctgta acggtgaaga gagcggcttc ataagcgatt
     4621 ttatccttgc ctgattaacg ttttccgctg tgcaaccaga acggttcctg attgtttgcc
     4681 tcattacgga aatgctcaat ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc
     4741 tgccagttct ccagccaggc atgcgcgacg acaggatcat agtaacccgc cagcaacagt
     4801 attgagtcaa tgtcacgcgt caggcgtggc aactggtcac ggtagagatc gcctaacggc
     4861 tggcagaaaa cgcttttcag ttcagcggca tggtgggaaa gatggatatc ggcaaagcgt
     4921 ttgaaggagt cgctgatttt gccctgcgct ttggcatcta aaaatggctg ccatgctttg
     4981 cttaccaacc attcggtcaa cgccagcttc gccattgccg tttcggtaga gtagaccgcc
     5041 gtcacggcag aaaccgcaga agcaatggtc gcttcgcatt gggtcagcaa atcacgtaag
     5101 tgagtgctcg ctttacgcgg cacaatacca ccgaacagca tcagcgtatg acggaccagg
     5161 ctaatggctg ccagcacctg ttctttcgcc gcatcgttac cgcgtaccca cagttcttca
     5221 tgatactgcc attgcgctaa cgccagctcg agcgccgctt ccagcccctg ttctacatcg
     5281 gcttttgccg caacatgcaa aatggtggtc ggtttgattt cacgcgccgg attgccctgc
     5341 gccagatgat agccacgcgc cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat
     5401 accagttggt tcgccagttt cagcaccgcg cgcgtgtcgc cgctaagcag ttccagttcc
     5461 agctcacaga taggttcagc aaattccccc gctttcacct ccccctgatc gagagcgatt
     5521 tcaattcggc tgccatcgac cgccaccagc catttttcgc gataaaaatc ggtgctgaac
     5581 agcggttgta cgcgggaagc gagatcggca ggcaattcac cgttcggcca gacttccgag
     5641 ggaaactgcg ccaggtcgag cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg
     5701 tgtaagccgc ctgtcactct tcctgcaact ttcatggtca tctcatagcg accgttttcg
     5761 ccacgaatac gtaagcccat atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa
     5821 atattcagca actgcacggg gtcatggtgc tcgccgccca gcgtattgag atggtcacgc
     5881 aacgcctcaa cggcactgtg attaacaata aactttaatt cgatttcctg agccatggcc
     5941 tttacttatc ggttatgtca cataagtgac gatgaacggc gaacttaatg cgatcttttt
     6001 tgtcagtaga tagtattttg cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa
     6061 agtttgaagc agtggcaaga cctgacacag aggattccga ttgatgtcga atgctttgcg
     6121 tagcgacact gatgccacta ctatcgttcc actttccatg acaataacga cagcctgatg
     6181 ccaaaattac gcctgatcgg attaacttta ctcgcactta gcgcgactgc cgtctcacac
     6241 gctgaagaaa cgcgctatgt ttctgacgaa ctgaatacct gggtccgtag cggtccggga
     6301 gatcattatc gcctcgtggg cacggttaac gccggcgagg aagtgacctt attacaaact
     6361 gacgccaaca ccaattatgc ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg
     6421 ttgaaacaac ttagcactga gccaagcctg cgctcccgtg tgccagatct ggaaaatcag
     6481 gtcaaaaccc tgaccgataa actcaccaat atcgataaca cctggaatca gcgcaccgca
     6541 gaaatgcagc aaaaagtggc gcagagcgac agcgtgatca acgggttaaa agaagaaaat
     6601 cagaaactga aaaacgagct gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag
     6661 ctggatgaca aacagcgcac catcatcatg caatggttta tgtatggcgg tggcgtgctg
     6721 gggcttggct tgctgctcgg tctggtactg ccgcacctga tcccaagccg caaacgcaaa
     6781 gatcgctgga tgaactaaat cgccttcttc gttgcactgt cttattatta ggaattgtgt
     6841 atttgacgaa gggatgatgg cgtgaagatt tatctggtcg gtggtgctat tcgggatgca
     6901 ttgttagggc taccggtcaa agacagagat tgggtggtag tcggcagtac gccacaggag
     6961 atgctcgacg cgggctacca gcaggtaggc cgcgattttc ctgtgtttct acatccgcaa
     7021 acgcatgaag agtatgcgct ggcacgtacc gaacggaaat ccggttccgg ttacaccgat
     7081 tttacttgct atgccgcacc ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg
     7141 accattaatg cgctggccca ggacgataac ggcgagatta tcgacccgta caacggtctg
     7201 ggcgatctgc aaaatcgtct gttgcgccat gtttcccccg cttttggcga agatccgtta
     7261 cgcgtactgc gcgtggcgcg ttttgctgcg cgttatgccc acctcggttt tcgtattgcc
     7321 gatgaaactc tgacgctgat gcgcgagatg acccatgcgg gtgaactgga acacctgacg
     7381 cctgaacggg tatggaaaga gacggaaagc gccctcacca cccgcaatcc acaggtgttc
     7441 ttccaggtac tgcgcgattg cggcgcactg cgcgttttat tcccggaaat tgacgcactg
     7501 tttggcgttc cggcccctgc caggtggcat ccggaaatcg acacgggtat tcatacctta
     7561 atgacgctct caatggcggc gatgctgagt ccgcaggtcg atgtccgttt cgcaacttta
     7621 tgccacgatc tcggtaaagg actgacgccg ccagaacttt ggccgcgtca tcatggtcac
     7681 ggtccggcgg gtgtgaaatt agtggaacaa ctctgccagc gtctgcgcgt gccaaatgaa
     7741 attcgcgatt tagccagact ggtggctgag tttcacgatc tcatccacac cttcccaatg
     7801 ctgaacccaa aaactatcgt caaattgttt gattccatcg acgcctggcg taaaccgcag
     7861 cgtgtcgagc aactggcgct gaccagcgag gctgacgtac gcggcagaac cggttttgaa
     7921 tcagcggact acccgcaagg ccgttggttg cgcgaagcct gggaagtggc gcagtcagtg
     7981 ccgacaaaag cggtcgttga agcgggattt aaaggtgtgg agattcgcga ggagctgacc
     8041 cgacggcgga ttgcggcggt agccagttgg aaggaacaac gttgcccaaa gcctgactga
     8101 gtataaaact aaacgttaac acgaaccatc ccctcgcccc ttcggggaga gggttagggt
     8161 gaggggaaca gaccagcacc ggtgcgaaca atcaccccca ccccggccct ctccctacag
     8221 agcgagggag aaaaccaggc ctgctcccga accttttctc aatcaaaaga acacgacgta
     8281 caccgcagcc gccacaataa agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat
     8341 caattgcagg aaggttttaa tcgctatcag cgccaccaca aaagcggtga taaacccaac
     8401 ggcaaacatc gagatatcgc cggttgtcag gaagccccag cttttgtaga gatcgagcgc
     8461 cgttgcgccc atcatcatcg gtaccgccag caggaacgag aactcggaag cagcgtaacg
     8521 gctcacgccc atcagcatcc cacctgaaat ggtggccccg gaacgggaga aacccggcca
     8581 cagcgccaga cactggaaac agccaatcat aaacgcctga cgataggtca tatcatcaag
     8641 acccggcgca cgcggctctt tcggcttcag gcattcggcg gcaatcagca acaaaccgcc
     8701 aacgaccagt gcatacatca catttatcgg gttaaacaat gacttaatcg tgtcgtggaa
     8761 caacagcccc agtaccaccg ccggaatcat ccccagcaaa atgtggatca gcgttaaacg
     8821 acctttgctt tctccttcgt gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa
     8881 cagacgccgc cagaacatca ctactaccgc cagaattgat cctaactgga tcacaacttc
     8941 aaaggttttc gccgtgtcgc cctcgaaccc caacaagtga ccgacaataa tcatatggcc
     9001 cgtgctggat accggcagaa attctgtcaa tccttcgacc acacccaata ttgccgctat
     9061 cagcagcgag tgcatatcgc tcatcaataa acccctaaat tattaaaatg taccgcttgt
     9121 ccggactact gcagtaagac caggttataa ccgtttggtt taacagctgt aaaattaatt
     9181 attttctttc agattattgc cacgctcaat gattacacca acattcgccg cccgcgccat
     9241 tgcgcctggc ttgctgagtt tgatgcgcac ccacggcgag ttgaagcgtg ctaacagcag
     9301 ctccgccacc tcttcagcca cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct
     9361 gaccaccgtt tctgcaatgt cagcgtaact gaggcaatcc gccacatcat cacttttcgc
     9421 cgctttacgg ttatcccatg ccatttcgat atcgaacact aacttctgtt cgatggtctg
     9481 ttcccagtcg taaacaccaa tagtggtgat taccgaaagt tgctctataa atacaatatc
     9541 catcacgtcc tgcctgcttt ttggctaacc ggataccact tccggcgaaa tgtgcgtatt
     9601 atccacagat tcatcgttga acacgaattt tcaaaacgga acagcttatg agtgcaatcg
     9661 cgcctggaat gatcctcatc gcgtacctct gcggctccat ttccagtgcc attctggttt
     9721 gccgcttgtg tgggctgccc gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca
     9781 atgtgttacg tatcggtggc aagggagcag ccgtagcagt actgattttc gacgttctga
     9841 aaggaatgtt gcccgtctgg ggcgcgtatg aattaggtgt cagccccttc tggttaggct
     9901 taattgccat cgccgcctgt cttggacaca tctggcccgt tttcttcgga tttaaaggag
     9961 gaaaaggcgt tgctaccgct tttggtgcca tcgcacccat tggctgggat ctcaccggag
    10021 taatggcggg aacctggtta ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga
    10081 ttgtcagtgc actgattgct ccgttttatg tctggtggtt taagccacaa ttcaccttcc
    10141 cggtttcgat gctctcttgc ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt
    10201 ggcgtcgtca ggagacaaaa atctggacga aattcaaaag aaagcgcgaa aaggatcccg
    10261 agtgatttct ggtggatcta catgacctga tagccttcat cgggcttgcc cagccgttgc
    10321 tggcaccatg ccgccagaaa ttccacgcag acacgtaatt tcatgctgcg atagagcggc
    10381 tcccggtaaa cagcccagat attggcgctt tgtgcatact ccggcaatac ttgcaccagt
    10441 ttgccactct ccagaaacgg caacacatcc cactcggaac gcagcataat ccctttgccc
    10501 tccagcgccc attgcaacac tatctcgccg ctattggagg aaagatgccc gctaaccttc
    10561 accgattttt tctcctgacc gttccccaac tcccatattc cgtgggtcat atcgcgctct
    10621 ttggtcacca gacagtcatg acgacttaat tcctgtaatg attgtggctg cggatatttt
    10681 tgcagatact caggtgctgc gcataatatt cttttatttt tcgttaacag atgcgcaata
    10741 taataatcag gaatttcgtc attaatacga atatccagat caatattatc ctgcactaaa
    10801 tcaatttgcc gatcgaacaa ttcaaaatgc acctgtaact caggataatt gcgcataagt
    10861 tcggtaatgg ctggcgcaat atggctgcgc ccaaaaccaa aactgcaacc aatacgaatc
    10921 atcccctccg ggcgcgtttt gatttgcgtg acgtcatcga ccagtcgctg atattgggtg
    10981 aggatttcca gcgcatgttc gtaacaacgt tggccacttt cagtcagcgc cacgccgcga
    11041 gcggagcggt tcagcagcgt tgttgccagc gtattttcaa ggatttggat ccgcttggtg
    11101 acaaatgcag gcgtctgccc aagcgtcgcc gctgcggcgc taaaactccc gctgtggacg
    11161 atttctacca gtacctgcaa gtctttggct aaaggccagc tattcagcat cgttaattat
    11221 ccgcagttgt gataagcgca gtgtatttcg caaaacattg ccattccctg ctctggcgca
    11281 atattcattc ttattttcgc gttgattcac gaattgttaa ttacatattc acggtggcaa
    11341 aaaatataaa accacatttt tagtggtagt ttgtggcggt gaatttttcc agacaaatac
    11401 aaaaactgga gttgccatga tgagcgaaag taataagcaa caggcagtga ataagttgac
    11461 ggagattgtc gctaacttta ccgccatgat ttctacccga atgcctgatg acgtggtgga
    11521 taaactaaaa cagctaaagg atgccgaaac gtcgtcgatg gggaaaatta tctaccatac
    11581 gatgttcgac aacatgcaaa aagcgattga cctgaatcgt cctgcctgtc aggacaccgg
    11641 ggagattatg ttcttcgtta aagtcggttc ccgcttccca ctgcttggcg agctgcaaag
    11701 catactcaaa caagccgtgg aagaggcaac cgtcaaagcg ccactacgtc acaatgcggt
    11761 agaaattttt gacgaagtaa acaccggcaa aaataccggt agcggcgtac cgtgggtcac
    11821 ctgggacatc atccccgaca atgacgatgc ggaaatcgaa gtttacatgg caggcggcgg
    11881 ctgcacgcta cctggccgct cgaaagtgtt aatgccgtca gaaggctacg aaggcgtggt
    11941 gaaattcgtc ttcgaaaata tctccaccct cgccgtaaac gcctgtccac cggtactggt
    12001 gggcgtgggc atcgccacct cggtggaaac cgccgccgta ctctcgcgta aagccatttt
    12061 gcgcccgatt ggcagccgcc atcccaatcc aaaagcggca gaactggagc tacgcctgga
    12121 agaaggactc aaccgtctgg ggattggtcc acaagggctg actggcaaca gttcagtgat
    12181 gggcgtacat atcgaatctg ccgcccgcca tccgtcaacc atcggcgttg ctgtctctac
    12241 cggctgctgg gcgcatcgtc gcggcacact gctggttcat gccgatctca cctttgaaaa
    12301 tctgtctcac acccggagcg cgttatgaaa aagatcctga caaccccgat caaagctgaa
    12361 gatctgcaag atattcgcgt cgacgatgtg atctacctga ccggtacgct ggtgacctgc
    12421 cgtgacgttt gtcaccgccg cttgatcgaa ctgaaacgtc cgatccctta cgatctcaac
    12481 ggcaaagcga ttttccacgc tggccccatc gtgcgcaaaa acggcgataa atgggagatg
    12541 gtttccgttg gcccgacaac cagtatgcgt atggaaagtt ttgaacgtga atttatcgag
    12601 cagaccggcg tgaaactggt ggttggcaaa ggcggaatgg ggccgctgac cgaagaaggc
    12661 tgccagaaat tcaaggcgct acatgtgatt ttcccggcag gctgcgcggt gctggcggca
    12721 acccaggtgg aagagattga agaagtgcac tggacagagc tcggaatgcc ggagtcgctg
    12781 tgggtctgtc gggtcaaaga gttcggcccg ctgattgtct ctattgatac ccacggcaac
    12841 aacctgatag ccgaaaacaa aaagctgttc gccgaacgcc gcgatcccat cgtggaagag
    12901 atctgcgagc acgtccatta catcaaataa ccctcccgga gaggctcgcc ccctctcctt
    12961 tttcgcaggc ataacacgat gaaaccttcc actgaatggt ggcgatacct tgcgccgctg
    13021 gcggtcatcg ccattattgc tctaattccg gttcccgcag ggctggagag tcatacctgg
    13081 ctctactttg ccgtttttac tggcgtgatc gttggactga tcctcgaacc cgtgccgggt
    13141 gccgtggtgg cgatggtggg tatatccatc atcgccatac tctctccctg gctgctgttc
    13201 agcccggagc agctcgctca gccaggcttt aaattcactg caaaatccct ctcgtgggcc
    13261 gtttccggtt tttctaattc ggttatctgg ctgattttcg ccgcctttat gtttggcaca
    13321 ggctatgaaa aaaccgggct tggacgccgc attgcgctga ttctggtgaa aaagatggga
    13381 catcgcacgc tgtttctcgg ctatgcggtg atgttctccg agctgatcct cgcacctgta
    13441 acaccgtcca actcggcgcg tggtgcgggg attatctatc ccatcatccg taacctgcca
    13501 ccgctctatc aatcacaacc aaacgacagc agttcgcgca gcattggctc gtacatcatg
    13561 tggatgggga ttgttgccga ctgcgtgacc agcgccattt tcttgacggc gatggcacca
    13621 aacttgctgt taattggact gatgaaaagc gcatctcacg ccacgctgag ttggggcgac
    13681 tggttcctcg ggatgttgcc gctcagtatt ttgctggtgc tgctggttcc ctggctggct
    13741 tatgtgctgt acccgccggt actgaagtct ggcgatcagg tgccgcgctg ggcagagacg
    13801 gaactgcagg caatgggccc gctctgttcg cgtgaaaaac ggatgctggg gctgatagta
    13861 ggcgcgctgg tgctgtggat tttcggcggt gattatatcg atgccgcgat ggtcggttac
    13921 agcgtggtgg cactgatgct gcttctgcgc attatcagtt gggacgacat tgtcagtaat
    13981 aaagcggcgt ggaacgtttt cttctggctg gcctcgctta tcaccctcgc taccggactc
    14041 aacaacaccg gttttattag ctggtttggc aaactgttag caggcagctt aagcggttat
    14101 tcgccaacga tggtgatggt ggcgttgatt gtggtgtttt atctgctgcg ctactttttc
    14161 gccagcgcca cggcgtatac ctccgccctc gcaccgatga tgattgccgc tgcgctggcg
    14221 atgccggaaa tcccgctgcc ggtgttctgc ctgatggttg gcgcggcaat tggtctgggg
    14281 agcattctta caccatacgc caccggaccc agtccgattt actacggtag tggttatctg
    14341 ccaacggcgg attactggcg actgggggcg atttttgggc tgatattcct cgtgttgctg
    14401 gtgattaccg gcttactgtg gatgcccgtg gtgttgcttt aagtgaggac ggtgccggaa
    14461 attgctctcc ggcacccaat agttttacgc agccggtaac tccgccagcg gccagcgcgg
    14521 acgcacgcta acgccgagat ccgccgtcgc gcctgcttta aaccgcacca ttccggcata
    14581 ggcgatcatc gcgccgttat cagtacaaaa ctccggacgc gcgtagaaca cttcgccgcg
    14641 gcgttttttc atcatttcag ccagcttcgc ccgtaacgtg cggttagcac tcacgccgcc
    14701 cgccatgacc agtcgcttaa agcccgtctg atccaacgct cgcttacact taatcatcaa
    14761 cgtatcgacc accgcatctt caaaggcgcg ggcgatatca gcacgcgtct ggtcgtcggt
    14821 gccgttgtca cgaatcgtgt tcgccgcaaa ggttttcaga ccagaaaagc tgaaatccag
    14881 ccccggacgg tcggtcatcg gacgcgggaa aacaaagcgc ccggcagtac cctgagccgc
    14941 cattttcgac agtaacggtc cgccaggata atccagcccc agcagcttcg cggttttatc
    15001 aaacgcttca ccggcggcat catcgataga ctcgccgagc agctcgtact gaccaatgcc
    15061 agtcacgctg attaactgcg tatgaccgcc ggacaccagc agcgcgacaa acggaaattc
    15121 cggcgggtta tcttccagca tcggcgctaa cagatgccct tccatatggt gtaccgggat
    15181 tgccggaacg ttccaggcaa acgccagaga acgccccacg gttgcgccaa ccagcagtgc
    15241 gccgactaat ccagggcctg cggtataggc cacagcatca atgtcttttg ccgttaagcc
    15301 agactccttt agcgccgcct ggatcaacgg tacggttttg cgcacgtgat cgcgggaggc
    15361 cagttcaggc acgacgccgc catagtcagc gtgcaatttc acctgactat acaattggtt
    15421 ggctaacaaa cctttttcat cgtcgtaaat ggcgatgccg gtttcatcgc aggaagtttc
    15481 aatacccagt acacgcatga cttgttttac ctcgctttat taccgcgcag tgtaggacca
    15541 atgcgggttg atgtaaaact ttgttcgccc ctggagaaag cctcgtgtat actcctcacc
    15601 cttataaaag tccctttcaa aaaggccgcg gtgctttaca aagcagcagc aattgcagta
    15661 aaattccgca ccattttgaa ataagctggc gttgatgcca gcggcaaacc gaattaatca
    15721 aaggtgagag gcacatgccg gtaattaaag tacgtgaaaa cgagccgttc gacgtagctc
    15781 tgcgtcgctt caagcgttcc tgcgaaaaag caggtgttct ggcggaagtt cgtcgtcgtg
    15841 agttctatga aaaaccgact accgaacgta agcgcgctaa agcttctgca gtgaaacgtc
    15901 acgcgaagaa actggctcgc gaaaacgcac gccgcactcg tctgtactaa ttccccgaga
    15961 gcgttgctct ccgatcagac cgagttgtag ttgtaaggcc gtgcttccga aaggaatgcg
    16021 cggcttattt tcgtttatga attgctaaaa atcggggcct atggctggac gaatcccacg
    16081 cgtattcatt aatgatctgc tggcacgcac tgacatcgtc gatctgatcg atgcccgtgt
    16141 gaagctgaaa aagcagggca agaatttcca cgcgtgttgt ccattccaca acgagaaaac
    16201 cccgtcattc accgttaacg gtgagaaaca gttttaccac tgctttggat gtggcgcgca
    16261 cggcaacgcg atcgacttcc tgatgaacta cgacaagctc gagttcgttg aaacggtcga
    16321 agagctggca gcaatgcaca atcttgaagt gccatttgaa gcaggcagcg gccccagcca
    16381 gatcgagcgc catcagaggc aaacgcttta tcagttgatg gacggtctga atacgtttta
    16441 ccaacaatct ttacaacaac ctgttgccac gtctgcgcgc cagtatctgg aaaaacgcgg
    16501 attaagccac gaggttatcg ctcgctttgc gattggtttt gcgccccccg gctgggacaa
    16561 cgtcctgaag cggtttggcg gcaatccaga aaatcgccag tcattgattg atgcggggat
    16621 gttggtcact aacgatcagg gacgcagtta cgatcgtttc cgcgagcggg tgatgttccc
    16681 cattcgcgat aaacgcggtc gggtgattgg ttttggcggg cgcgtgctgg gcaacgatac
    16741 ccccaaatac ctgaactcgc cggaaacaga cattttccat aaaggccgcc agctttacgg
    16801 tctttatgaa gcgcagcagg ataacgctga acccaatcgt ctgcttgtgg tcgaaggcta
    16861 tatggacgtg gtggcgctgg cgcaatacgg cattaattac gccgttgcgt cgttaggtac
    16921 gtcaaccacc gccgatcaca tacaactgtt gttccgcgcg accaacaatg tcatttgctg
    16981 ttatgacggc gaccgtgcag gccgcgatgc cgcatggcga gcgctggaaa cggcgctgcc
    17041 ttacatgaca gacggccgtc agctacgctt tatgtttttg ccggatggcg aagaccctga
    17101 cacgctggta cgaaaagaag gtaaagaagc gtttgaagcg cggatggagc aggcgatgcc
    17161 gctttccgca tttctgttta acagcctgat gccgcaagtt gatctgagta cccctgacgg
    17221 gcgcgcacgt ttgagtacgc tggcactgcc attgatatcg caagtgccgg gcgaaacgct
    17281 gcgaatatat cttcgtcagg aattaggcaa caaattaggc atacttgatg acagccagct
    17341 tgaacgatta atgccaaaag cggcagagag cggcgtttct cgccctgttc cgcagctaaa
    17401 acgcacgacc atgcgtatac ttatagggtt gctggtgcaa aatccagaat tagcgacgtt
    17461 ggtcccgccg cttgagaatc tggatgaaaa taagctccct ggacttggct tattcagaga
    17521 actggtcaac acttgtctct cccagccagg tctgaccacc gggcaacttt tagagcacta
    17581 tcgtggtaca aataatgctg ccacccttga aaaactgtcg atgtgggacg atatagcaga
    17641 taagaatatt gctgagcaaa ccttcaccga ctcactcaac catatgtttg attcgctgct
    17701 tgaactgcgc caggaagagt taatcgctcg tgagcgcacg catggtttaa gcaacgaaga
    17761 acgcctggag ctctggacat taaaccagga gctggcgaaa aagtgattta acggcttaag
    17821 tgccgaagag cgatcgggaa gcccccgaca gccgcactga gaggcagcgg caaatatata
    17881 agtacgccct cgtaattatc gttggcggta aacaaccgtt ggatttcagc gttaacggct
    17941 gaaggacatc gggtcaatcg cccaacacca acctcatgaa ataagtgtgg ataccgtctt
    18001 atggagcaaa acccgcagtc acagctgaaa cttcttgtca cccgtggtaa ggagcaaggc
    18061 tatctgacct atgccgaggt caatgaccat ctgccggaag atatcgtcga ttcagatcag
    18121 atcgaagaca tcatccaaat gatcaacgac atgggcattc aggtgatgga agaagcaccg
    18181 gatgccgatg atctgatgct ggctgaaaac accgcggacg aagatgctgc cgaagccgcc
    18241 gcgcaggtgc tttccagcgt ggaatctgaa atcgggcgca cgactgaccc ggtacgcatg
    18301 tacatgcgtg aaatgggcac cgttgaactg ttgacccgcg aaggcgaaat tgacatcgct
    18361 aagcgtattg aagacgggat caaccaggtt caatgctccg ttgctgaata tccggaagcg
    18421 atcacctatc tgctggaaca gtacgatcgt gttgaagcag aagaagcgcg tctgtccgat
    18481 ctgatcaccg gctttgttga cccgaacgca gaagaagatc tggcacctac cgccactcac
    18541 gtcggttctg agctttccca ggaagatctg gacgatgacg aagatgaaga cgaagaagat
    18601 ggcgatgacg acagcgccga tgatgacaac agcatcgacc cggaactggc tcgcgaaaaa
    18661 tttgcggaac tacgcgctca gtacgttgta acgcgtgaca ccatcaaagc gaaaggtcgc
    18721 agtcacgctg ccgctcagga agagatcctg aaactgtctg aagtattcaa acagttccgc
    18781 ctggtgccga agcagtttga ctacctggtc aacagcatgc gcgtcatgat ggaccgcgtt
    18841 cgtacgcaag aacgtctgat catgaagctc tgcgttgagc agtgcaaaat gccgaagaaa
    18901 aacttcatta ccctgtttac cggcaacgaa accagcgata cctggttcaa cgcggcaatt
    18961 gcgatgaaca agccgtggtc ggaaaaactg cacgatgtct ctgaagaagt gcatcgcgcc
    19021 ctgcagaaac tgcagcagat tgaagaagaa accggcctga ccatcgagca ggttaaagat
    19081 atcaaccgtc gtatgtccat cggtgaagcg aaagcccgcc gtgcgaagaa agagatggtt
    19141 gaagcgaact tacgtctggt tatttctatc gccaagaaat acaccaaccg tggcttgcag
    19201 ttccttgacc tgattcagga aggcaacatc ggtctgatga aagcggttga taaattcgaa
    19261 taccgccgtg gttacaagtt ctccacctac gcaacctggt ggatccgtca ggcgatcacc
    19321 cgttctatcg cggatcaggc gcgcaccatc cgtattccgg tgcatatgat tgagactatc
    19381 aacaaactca accgtatttc tcgccagatg ctgcaagaga tgggccgtga accgacgccg
    19441 gaagaactgg ctgaacgtat gctgatgccg gaagacaaga tccgcaaagt gctgaagatc
    19501 gccaaagagc caatctccat ggaaacgccg atcggtgatg atgaagattc gcatctgggg
    19561 gatttcatcg aggataccac ccttgagctg ccgctggatt ctgcgaccac cgaaagcctg
    19621 cgtgcggcaa cgcacgacgt gctggctggc ctgaccgcgc gtgaagcgaa agttctgcgt
    19681 atgcgtttcg gtatcgatat gaacaccgac cacacgctgg aagaagtggg taaacagttc
    19741 gacgttaccc gcgaacgtat ccgtcagatc gaagcgaagg cgctgcgcaa actgcgtcac
    19801 ccgagccgtt ctgaagtgtt gcgtagcttc ctggacgatt aatcggtagg ccggatcagg
    19861 cgttacgccg cacccggcac tagtccctct gcacaaacgc cacctttt
//
LOCUS       DAZPLI010000071        19530 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZPLI010000071 DAZPLI010000000
VERSION     DAZPLI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.072"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..245
                     /locus_tag="ACHUU3_002389"
     CDS             <1..245
                     /locus_tag="ACHUU3_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFR2082424.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHUU3_002390"
     CDS             complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHUU3_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR2082425.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHUU3_002391"
     CDS             1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHUU3_002391"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFR2082426.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /gene="otnK"
                     /locus_tag="ACHUU3_002392"
     CDS             2164..3426
                     /gene="otnK"
                     /locus_tag="ACHUU3_002392"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFR2082427.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDDLDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNLQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="ACHUU3_002393"
     CDS             3423..4061
                     /locus_tag="ACHUU3_002393"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR2082428.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="ACHUU3_002394"
     CDS             4066..4842
                     /locus_tag="ACHUU3_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFR2082429.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="ACHUU3_002395"
     CDS             4931..6295
                     /locus_tag="ACHUU3_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFR2082430.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHUU3_002396"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHUU3_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR2082431.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHUU3_002397"
     CDS             complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHUU3_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR2082432.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHUU3_002398"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHUU3_002398"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR2082433.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHUU3_002399"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHUU3_002399"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR2082434.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHUU3_002400"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHUU3_002400"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR2082435.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHUU3_002401"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHUU3_002401"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR2082436.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHUU3_002402"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHUU3_002402"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR2082437.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHUU3_002403"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHUU3_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR2082438.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="ACHUU3_002404"
     CDS             complement(12844..13167)
                     /locus_tag="ACHUU3_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR2082439.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHUU3_002405"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHUU3_002405"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR2082440.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHUU3_002406"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHUU3_002406"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR2082441.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHUU3_002407"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHUU3_002407"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR2082442.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="ACHUU3_002408"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="ACHUU3_002408"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR2082443.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   17469..17741
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=17591..17619
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(17838..18131)
                     /gene="cas2e"
                     /locus_tag="ACHUU3_002409"
     CDS             complement(17838..18131)
                     /gene="cas2e"
                     /locus_tag="ACHUU3_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFR2082444.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18128..19051)
                     /gene="cas1e"
                     /locus_tag="ACHUU3_002410"
                     /pseudo
     CDS             complement(18128..19051)
                     /gene="cas1e"
                     /locus_tag="ACHUU3_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
     gene            complement(19048..>19530)
                     /gene="cas6e"
                     /locus_tag="ACHUU3_002411"
     CDS             complement(19048..>19530)
                     /gene="cas6e"
                     /locus_tag="ACHUU3_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR2082445.1"
                     /translation="RFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTIC
                     KAGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQ
                     QQLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA
                     "
BASE COUNT         4605 a         5269 c         4895 g         4761 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggcc cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgatct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaacctcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gtccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt gtgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgcgcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgcgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgggca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca ggttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgatcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggca gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacacc ctgacgcgcc gcagtattta
    17641 tctgctctgg ccggtttatc cccgctggcg cggggaacac gggtgcgtgt ggctgccagt
    17701 gccggagaac ggcggtttat ccccgctggc gcggggaaca cactaagcat acataactgt
    17761 ttttaaacaa atttattccg catcaacaat ctaccaacta aattaaaaca tttccttatt
    17821 tttaaagaac ggataactta ttgattatca acaggaagaa aagaaaccaa acgtaaccca
    17881 tccaaatcca ccggaatacg tctgttttct ccccaggtct gaaattcaaa acccgactcg
    17941 atattggtcg cccaggccat caccacattt ccgcaaccag ccagttgggt aatttgctgc
    18001 cagatcatct cccgaatacg ttttgatgta tcaccaacat acacaccggc acgcacttcc
    18061 agtagccaga ttgcgagccg tccacgtaag cgcggaggga cattttctgt aacaaccacg
    18121 accatgctca tccgccgcgc ccccggtgac cactatcacc cagcgtttca ggttcaggga
    18181 tggcaggcgg taacatatcc ggcgcgggtt gtggtggttc aatttcacct gcagcaagga
    18241 cttcctcaat taacggtatt aatttgcccg ttaacttagt gctacggaaa atatcgcgac
    18301 aggctaatct gacttcttta tcaggttctg cgggttgcct cgctgctatt tcaaatgcct
    18361 ttggcacaac cgaatcaaat ttaatgatat cggctatgtc ataaacaaat gaaagcggtt
    18421 tgccactatg gataaaccca atagcgggcg catatcccgc ggctaatact gccgcttcag
    18481 atataccgta cagacatgat gtagcagcac tgatgcagcg attcacaaca tcgccttttt
    18541 cccagtcttt aggatcgtat ttgcgaccat tccatttcac accatattgt ttcgccagta
    18601 atgcatacgt ctggcgaacg cgagatccct caattccacg taactgctcc actgaacggc
    18661 gagctggcgg tggttcacga aaacgtaatt catacatttt gcgcaccacc ttcaaccgta
    18721 gatcttccgt taaagccagc tttgcctagt agagtaattt atctgcccgc gcccctccgg
    18781 gttgtccgga ggagtaaacg cgaacgcccg cttcaccgac ccagaccagc agtgttccca
    18841 ccgtggcggc cagatggact gccgcgtggg aaactcttgt tcccggttcg agcataatgc
    18901 aggcgaccga tcccactgga atatgcgtgc ggatcccggt tttgtcgatc agcacgaaag
    18961 cgccgtccag tacgtcgatt tgaccgtact ggaggaagat catggaagtg cgatctttta
    19021 atgggatcgg gctcagcggt acaaacgtca cgcctctgct ccgggtttga tcagcatcag
    19081 accacaaccg aacgcccggc ttttgccata cccctgactg agacgttgca gaaataaccc
    19141 aggagcggtg accgtaagca tccccgtata atccacgcta ctgaactgga tcagttgccg
    19201 ggagttttcc cgccgcagtt gctgttgtcg gtaggcatca accgaagtat ccagcagtgt
    19261 aaaaccgctt ctctccccct gcgctgccag ccagtccagc gcagcctgtt gttgatgcaa
    19321 ccagacatca cttccttcca cctgccccct cacctgccgt ttcgcctcca tcagcaagtc
    19381 gtggcgtttg cccgctttac aaattgttgg attcgcccgc aggttgaaac aaagggattg
    19441 tccggtacgc aactctggcg caaatggtcg gcattcgatg gtgaaaatat cactttccgc
    19501 cgggcgttcc tgcgacagga caaaaaagcg
//
LOCUS       DAZPLI010000072        19270 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZPLI010000072 DAZPLI010000000
VERSION     DAZPLI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19270
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.073"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..251)
                     /locus_tag="ACHUU3_002412"
     CDS             complement(<1..251)
                     /locus_tag="ACHUU3_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2082446.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNAENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPE"
     gene            complement(419..838)
                     /locus_tag="ACHUU3_002413"
     CDS             complement(419..838)
                     /locus_tag="ACHUU3_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2082447.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYVKSLINKDN"
     gene            complement(838..1128)
                     /locus_tag="ACHUU3_002414"
     CDS             complement(838..1128)
                     /locus_tag="ACHUU3_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2082448.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHTALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            complement(1449..1739)
                     /locus_tag="ACHUU3_002415"
     CDS             complement(1449..1739)
                     /locus_tag="ACHUU3_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2082449.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            complement(1739..2625)
                     /locus_tag="ACHUU3_002416"
                     /pseudo
     CDS             complement(1739..2625)
                     /locus_tag="ACHUU3_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(2989..3651)
                     /locus_tag="ACHUU3_002417"
     CDS             complement(2989..3651)
                     /locus_tag="ACHUU3_002417"
                     /inference="COORDINATES: protein motif:HMM:NF017666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFR2082450.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNRLTIRSPFPSAVVVFKKEPRKHEITQMVCCAD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKYIADALRMPLSTSYRIAQR
                     LS"
     gene            complement(4020..4571)
                     /locus_tag="ACHUU3_002418"
     CDS             complement(4020..4571)
                     /locus_tag="ACHUU3_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR2082451.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFADEAVSGSVK
                     ASLRNGFSSLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQMLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            5157..5504
                     /locus_tag="ACHUU3_002419"
                     /pseudo
     CDS             5157..5504
                     /locus_tag="ACHUU3_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            5557..5952
                     /locus_tag="ACHUU3_002420"
     CDS             5557..5952
                     /locus_tag="ACHUU3_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR2082452.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            5993..6736
                     /gene="cmoA"
                     /locus_tag="ACHUU3_002421"
     CDS             5993..6736
                     /gene="cmoA"
                     /locus_tag="ACHUU3_002421"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR2082453.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            6733..7704
                     /gene="cmoB"
                     /locus_tag="ACHUU3_002422"
     CDS             6733..7704
                     /gene="cmoB"
                     /locus_tag="ACHUU3_002422"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR2082454.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(7869..10298)
                     /gene="torZ"
                     /locus_tag="ACHUU3_002423"
     CDS             complement(7869..10298)
                     /gene="torZ"
                     /locus_tag="ACHUU3_002423"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR2082455.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKDGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTTRIQHPMVRKSYLDNPLQPT
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIDFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLRGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(10323..11423)
                     /locus_tag="ACHUU3_002424"
     CDS             complement(10323..11423)
                     /locus_tag="ACHUU3_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR2082456.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(11811..12557)
                     /gene="cutC"
                     /locus_tag="ACHUU3_002425"
     CDS             complement(11811..12557)
                     /gene="cutC"
                     /locus_tag="ACHUU3_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR2082457.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(12571..13137)
                     /locus_tag="ACHUU3_002426"
     CDS             complement(12571..13137)
                     /locus_tag="ACHUU3_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR2082458.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            13353..15089
                     /gene="argS"
                     /locus_tag="ACHUU3_002427"
     CDS             13353..15089
                     /gene="argS"
                     /locus_tag="ACHUU3_002427"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR2082459.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            complement(15131..15175)
                     /gene="yecV"
                     /locus_tag="ACHUU3_002428"
     CDS             complement(15131..15175)
                     /gene="yecV"
                     /locus_tag="ACHUU3_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFR2082460.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            15266..15754
                     /locus_tag="ACHUU3_002429"
     CDS             15266..15754
                     /locus_tag="ACHUU3_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFR2082461.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIENDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(15874..16266)
                     /gene="flhe"
                     /locus_tag="ACHUU3_002430"
     CDS             complement(15874..16266)
                     /gene="flhe"
                     /locus_tag="ACHUU3_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFR2082462.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(16266..18344)
                     /gene="flhA"
                     /locus_tag="ACHUU3_002431"
     CDS             complement(16266..18344)
                     /gene="flhA"
                     /locus_tag="ACHUU3_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFR2082463.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(18337..>19270)
                     /gene="flhB"
                     /locus_tag="ACHUU3_002432"
     CDS             complement(18337..>19270)
                     /gene="flhB"
                     /locus_tag="ACHUU3_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFR2082464.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         4876 a         4818 c         4350 g         5226 t
ORIGIN      
        1 tgctctgggg aaatactgta tatgttcgtt tgctcgataa ccaatctttt catgttgctg
       61 tctgtaattg gtagcatcat tcgaggctcg ttaaactcca ccgactgaat aaagtcagag
      121 atgttttctg cattatcagc catcagcttt agtaatttat caaaattaat tgcgatacct
      181 gcatcaatga agctttgcac catcatagca gtgtcgttat caaccataat ttgcattggg
      241 taattattca tttttaaatc tcctatgaaa cacacctcag acctcttaaa atagccattc
      301 tgaggccagt aaccatgcgg gttacagagg gagttgcact ggctggtaag gtaatttatc
      361 aaattaaggt gcaactctgc ctgtaaagtg cattctaccg tttcttagat ggttattttt
      421 aattgtcttt attaatcaat gacttaacgt agaaatactg tctccgtgaa ataccatatt
      481 tttctgtaat gtatcgctga gattgaccag catttatggc ctgagtgata tcatcacggt
      541 tatagcggtt accacgggat ttatatttgt caccatccac atcttctgag gtattgaagt
      601 cttcaatcag cttaattgca tcgtgaaggt catcttctga ggcataatcg aaggtatgga
      661 taatctcaaa atcaccagta tctgctggtg cattcttcca tctggagaag cgggatttta
      721 attgctggtg tgtgaagggt tcagcattac gagttaaatc tttccatgtt ccgttattct
      781 cacggacaag gtgacctcta tggcgataaa acgcatgata atcaaatgtg catagcatta
      841 aacccccagc ttatccgcca gttgatgaac cttggcactc aacacctgaa tcattgagca
      901 acctgccact gcggactgtg ctttgagtag ttccagttgg cccgttgtga ttctgatggc
      961 cttagaaccc tctgcaacgc ccttgatagc gtcaaacggt gtaagggctg tgtgttcctt
     1021 cagaatctca ccaacaagtg ctgtgtggct catggctggg tatttgtctt tgattgctgc
     1081 cagtctggaa tatgcctctg gcaaaaggcg tgtaactgtg tgtctcatta aattttcctt
     1141 cttaaaatct ttgacttata actaacttta acatatttat aacatattgt caacaactca
     1201 tttacatgtt aatatggttg tagggggctg atacaggtca ttccctttga taaataaggg
     1261 gaattaatgg gctttcttga aacaattgct gaatctcaca gattcgcaag cttaggttgc
     1321 tttgtgtttc tggtgcttct tgtgtatcag cttttaaaaa tgattatcgc cagaagtaca
     1381 cgattcaatt tcagcattaa gcttttcaaa tatgaaatca caattacgat tgatgatggt
     1441 aaaacaaact aacgttttgt gataatattg gtggccttta agttgctggg gtcaccgtct
     1501 tttaatcgta gctggtggat tgaattccag atatcaaaat gatacagcac tgcaaacctc
     1561 aaagcgtcaa tctttaaatc tgtcacacca tcgtaaaaga aataacccct cccttctttc
     1621 tttggtctaa tccggtaaac ttccccgtga gtatccttca taagatgacc ttcacgagaa
     1681 atgctgtaac gctttaacag gtaatctttg tatctctttt caccgtgata ttctttcatt
     1741 acttaattcc tccaccggca aaatacatct tcggtttaat tttttgcttc ttgtcatcct
     1801 gagtttgttt ctttttcggt gaaagataat aatcatcttc actgtcatcg aatgggtcta
     1861 aacccgatac tttgttcttt tgtcttgcaa gctcgaatct ctttttcttc tctgccctct
     1921 taatggcttt ttcacgttca tcctcttcat catcgaaggg gtccaactca tcatcagtgg
     1981 cggggcactg tgtgttttta tggtcactat caacactcac atcagtgatt tctctgtctt
     2041 gtttagaagt ctttttagag cttacttcaa ggtcagattc attcatagag agaaaatcat
     2101 ttaattcatc caaagaaaga aagtcatctt ctttaatatc atcattaagt ccagtctctt
     2161 tattcttatc tgtaatatta ttatctaatg ttttatctaa taacttatct ctctaacacg
     2221 ggtgacattt tgtcactacc cccatgacat tttgtcactg gtagtggtgt cattttgtct
     2281 tactgaggct gcaacttcct gtgatgtagg tcttacaagt aattccggct tatcatccag
     2341 tgtgaataca cgataagtgc tttgacgccc ctgattcttt tgcacctgaa ttaaaccgtg
     2401 tttctcaagt gtctcaatgg ctctgattgc tgttctcttt gatacaccag ccatataagc
     2461 gagatggtca atacttggtg aatatgattt gtatttctct tcatagaagt cacagctatg
     2521 gaccatgacc atataaatca atcttgctgc tgcgttaatt tcttctgaaa gttgagagat
     2581 tcgcacatca aatttataaa actgatttgg gttatattta gacattcata aattacctca
     2641 tacgtgaaaa ggccaaccgg ataaaaccga ttagcctgtg tgattaaaat atctggggga
     2701 ttgatggact gataaagaag tccatgcctg aaacagtacg atgattaaaa cgctgaggta
     2761 taccctgaat gagaggttaa tatacgccaa ccatctgaca cctccccaag tgttaccact
     2821 tcaagacatg aactttttta tcagcccata tagaaactat aacagatgtg attataaatt
     2881 gcaacaatta tttcacagaa tgattactga ttggtcattt attgttcaca gggatacttc
     2941 ttaacatctg aatcacctgt cttgtaaatg accatattta tgagaatttt agctcaacct
     3001 ttgagcaatc ctgtatgacg tgctcagagg cattctgaga gcatctgcaa tgtacttaat
     3061 gggttttcct tgcctgtata gttttatgaa gctgtcgagc gtctcaggtg ggtaaatggt
     3121 gggtcttcct cttagtccac cacggttaat gatggttaac ggtagcaatg tatcagcaca
     3181 gcaaaccatc tgtgtaattt catgcttacg tggttctttt ttaaaaacaa cgactgcact
     3241 tggaaacggt gacctaatag taagcctgtt atgccctgct ggtggaaggt atttaactgc
     3301 acgatgaaga atacggattt catgacagtg gttattgctg taaatgtgcc taagccagta
     3361 gctggtgttg gctctatgcg gtatcaggaa agctactaaa tccgcttcag acgcttttaa
     3421 gaggaacgac ggaatatcgc tgtacggcgg gttacagaag accttttcac taacccatga
     3481 ttgacggctt atatcatcgg tgaatcgtgg taacagcgtg tcatgctcag ttgcagcacc
     3541 atctagactg aaaataaatt cacggtgtaa gttcttaaat aacaaaaatg gagttctcca
     3601 gtaatcagaa cccccatgat gcttcccgat taaaatatgc tcaattgaca taagaaataa
     3661 aaatttttat tgcaatcact cttgacaagg tttactctct tttaagagat acttaacagg
     3721 ttttctaaac ggatatcctt tctgggatat ctatctttta tcttaaaagt ttttacagat
     3781 actttaaaga tatctgtata tattctgtac agtaactttt cagagtctga aaatttttat
     3841 aaaaaatgac tagccctctt tgaatctcat cttccccgaa tcttcgaaaa tatctgtcta
     3901 gttgattcgg tagaatctac ctcatgatgt atacgtgttt agtacccgtc acaccagcta
     3961 tgtgaccccc ttggcaaaaa agccaaatat cagctacatc catgtaacta ttcagtaact
     4021 tatttagcaa gtctgtaata agttgctcta gagattccta gctcttcctg aatctgcaac
     4081 ctcgttttac cttgtgaaat aagcatctgt acagcctctg aagaagcttt tacaggtctt
     4141 cccatatgta ccccttcagc tttagcacgt tcaataccag cctttctacg ctctgctatc
     4201 aagcttcttt caagctgtgc caacgttgac ataatgccta acattgcttc acccatagcg
     4261 cttgataagt caaatccttc tctaaggctg ataactgtca cacctttagc ctgtagcgct
     4321 ttaaccgttg ataacacatc aattgtgtca cgtccaagac ggtctacagc taccacaact
     4381 acggtgtcac cttctcgtgc ataagctaac aaactgctga aaccgtttct taaagaagct
     4441 ttgacagaac cactgacagc ttcatcagcg aaccatttag caatgttgta ccctgcctct
     4501 tcaatttgca tttgctggtt agtgatggat tggtcatctg tactcaccct gcaatatcca
     4561 agtatcgcca taatgttccc cttagaagtc tcaaaagtta gtctcataag tttagctgtc
     4621 tcataatcca aaaacaacct tttgagacta cttgagggtg cattctaccg ttacgttgaa
     4681 agtgtctcaa aaggactcct tatgagactg gttaatagag cgctatgaag attcaggagg
     4741 gtaactataa ccatcacttt ctaactttca cgtaggaaat cgttaagttt cttgatagtt
     4801 acaccatgtg accaactatc tatatatcgt tatatagtcc tgtatatagc actatatgta
     4861 gtgtctggag agggacgact aacacgatac agtttaccct gaaaagagac ttcacagatt
     4921 ctttatatat ttcaataagt tatgctaaaa agagactata ccgatactgc atagaatttg
     4981 tgtaggattg catagatact tggaaaaaca ccgttaaatt gcatagtcat tcacctgata
     5041 ttgtcctgtt tttacaggta gttgattcgg atgttctagg aatcatcctc atagttgatt
     5101 cggttagttg ggcttgtacc aacccctaat caacctcata gtgtatttag accttgatga
     5161 ttttagacag ccgccctgtt catgcagcac gtccacacag tgaagctatt cgcgacgctc
     5221 aacgaaaaaa acctaaagtt ccggtacatg ctgtactgac ggcgacaaat ccgctgattc
     5281 gttttatcgg tagtgatgat atgacgcaaa accgggaatt atttcaggtc tggttacaga
     5341 aattagcgca gtggcatcag accactacgc cttatctttt tttacatacg ccagacattg
     5401 cccaggcccc ggaactggta cataccctgt gggaagactt acgtaaaacg cttccagaga
     5461 tcggagcagt tccggctatt ccacagcaat cttctctttt ctgaatttgc cacctatcat
     5521 agacaggtgc catcggccat tttaaaggga gtttgtatgg taagcgcgct atatgccgtt
     5581 ttaagtgcgt tgttattaat gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac
     5641 cgcgttgcct atggtgacgg tggttttagc gaactgcaaa gcgctattcg cattcatggt
     5701 aacgcggtgg aatatattcc catcgcgatt gtgctgatgc tgtttatgga aatgaatggc
     5761 gcagaaacct ggatggtgca tatttgcggc atcgttttgc ttgctggtcg tctgatgcat
     5821 tattacggtt ttcatcaccg tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg
     5881 tgtgcgctgt tgctgatggt gctggcgaat ctttggtata tgccctggga gttggttttc
     5941 tccctgcgtt agcgcacaat acgccacttt ctttttcccg gatttttacg ttatgtctca
     6001 ccgcgacacg ctattttctg cccctatcgc cagactgggc gactggacct ttgatgaacg
     6061 ggtagctgaa gtcttcccgg atatgatcca gcgttccgtt cccggctatt ccaatattat
     6121 ttccatgatt ggtatgttag ccgagcgctt cgttcaacct ggtacgcagg tttacgatct
     6181 gggttgttct ctgggcgcgg cgacgctctc ggtgcgtcgc aacattcatc atgataattg
     6241 caaaattatt gccatcgaca actccccggc gatgattgaa cgctgcagtc gtcatattga
     6301 cgcctataaa gcccctacgc cagtagacgt cattgaaggt gatattcgcg atatcgccat
     6361 tgaaaacgca tcgatggtgg tgctgaattt taccctgcaa ttcctggaac cttccgagcg
     6421 ccaggcatta ctggataaaa tttatcaagg actgaacccg ggcggcgcgc tggtgctttc
     6481 ggaaaaattc agtttcgaag atgccaaagt tggtgaactg ttgttcaaca tgcaccacga
     6541 ctttaaacgt gccaacggtt acagcgaact ggagatcagc cagaagcgca gcatgctgga
     6601 aaacgtgatg ctgaccgatt ccgtggaaac ccataaagca cgcctgcata aagccggttt
     6661 tgagcatagc gagctgtggt tccagtgctt taactttggt tcactggtgg cattaaaagc
     6721 agaggacgct gcatgatcga ctttggtaac ttttattctc tgattgccaa aaatcatctt
     6781 tcacactggc tcgaaacgct gcccgcgcag attgctaact ggcagcgcga gcagcagcac
     6841 gggctgttta agcagtggtc caacgcggtg gaatttctgc ctgaaattaa accgtatcgt
     6901 ctggatttat tgcatagcgt aaccgccgaa agcgaagagc cactgagcac cgggcaaatt
     6961 aaacgcattg aaacgcttat gcggaacctg atgccatggc gcaaagggcc gttctcactg
     7021 tatggcgtca acatcgatac cgaatggcgt tccgactgga aatgggatcg tgttctgccc
     7081 catctttctg atttaaccgg acgcaccatt ctggatgtcg gctgcggcag cggttatcac
     7141 atgtggcgca tgattggcgc aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg
     7201 tttctgtgcc agtttgaagc ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg
     7261 ttaccgttag gtattgaaca gcttccggca ctgaaagcct ttgataccgt cttttcgatg
     7321 ggcgtgcttt accatcgccg ttcgccgctg gagcatctct ggcagttgaa agatcaactg
     7381 gtgaatgagg gtgaactggt gctggaaacg ctggttattg atggcgatga gaacacggtg
     7441 ctggtaccag gcgatcgtta cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg
     7501 gcgctgaaaa actggctgaa gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc
     7561 gttaccacta ctgaagagca gcgacgcact gaatggatgg ttaccgaatc tctgtccgat
     7621 ttcctcgacc cacatgatcc gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg
     7681 gtgctgattg cgcgcaagcc gtaaaggtct ggtattactg ccggatgcgg cgtgaatgcc
     7741 ttatccggcc tacaaaccct tgctaattca atctattgca gggactatgt aggcctgata
     7801 agcatagcgc atcaggcagt tttacgtttg cataacctca gcgcccgttt ccgggcgcta
     7861 ttcacttctt acgcctgaac agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc
     7921 gtatattttt cgatatacac cagcgcagag ttaccggcac aggcatttgc cagctgcgag
     7981 ctggggatat ccgccgttaa cacgttcgca ctgccgtttt tacacaagcc gttttccaga
     8041 tctggccatg caccttcatg cacgcatacc acgccttttt tgatcccgtc agtcaccacc
     8101 gcgcctgtca aaatctgacc acgtttgttc cagacacgca ccagatcgcc attcgcaata
     8161 ccaaatcgag cagcatcttc ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat
     8221 tttttacgca gttccgcata gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc
     8281 agaagctgca actgcttctc gtcggcggta cccttccact catcaggcgc aagccaggtt
     8341 gggtgtgccg ggcaatcctt atagccaaat ttttccagcg ttttggagta aatctcaatt
     8401 ttgccacttg gcgtacccag cgcatttttc accggatcgg cgcggaaatc accataacga
     8461 acgtactgtt cgttcttctc gctgcggcgc atttcgatca gtttattttg ctgccagaag
     8521 gcattaaaca ttggcatagt gacgcgttgc gcacgggccc ctttctgagc ggcatcatag
     8581 aaaaatttca gccacgccat ttcatcttta ccttcggtat agatctcttt tccgccaggt
     8641 ttgagtaatt ccgcaagatc ggcaaacaca tcaaaatcgt ttcgcgcttc aaattgcgga
     8701 gcaacagcct gcttcatcgg cacaatatgc tggttgctgt aatcaccggt catcgtcaag
     8761 tcattgcgct caaacgatgt ggtgatcggt aatacgatat ctgcgtgttt agcagctgcg
     8821 gtccagtagc attcagaaac gacgatcatc tccggtttct gccatgcttt aatcagacga
     8881 ttagtatcct ggtgatgggt aaagttaccg ccacccgccc accagatcat tttgatattc
     8941 ggatacgttt gctctttacc gttatgctga tatttgccgc caggattttc cagcgcatcg
     9001 acgatacggg caacaggaat cgccctcatt ccgccatcat cagcggcctc gctggcctgt
     9061 ccggcaatcg ccgcagacat ttccggcagc acgccgccaa cacgtgtcgg gttgccacca
     9121 ttggagtaat gataagagaa accaaaaccg ccgcccggtg taccaatctg ccctaacatc
     9181 gccgccagcg tcaccagcat ccagtgtttc tgttcaccat attgctggcg ctgaattccc
     9241 cagcctgcca tcagcattgt gcgattcgcc gccatcagtt cagcgagttt gactatctgc
     9301 gcttccggta cgccagtaat ttccgccgcc caggccgcgc tcttcggcgt attatcgctc
     9361 ttacctctca gatactcttc aaactgcgga tacccggtag tgtatttttc aaggaacact
     9421 ttatcgtgtt tgccttgtgt catcagggta tgcgcaatcc ctaacatcag tgccacgtcg
     9481 gtgcccatat tcggcgcgat ccaggtggca ttatcgccaa agaagtcgat ggtttcggag
     9541 cggataggat caatggcaat cactggtttg ccagattttt tcagctgatg gaagtattcc
     9601 agcccttgct catcggtact gctccaggca atttttaagg tattcagcgg gttcatcccc
     9661 cacagcacca caacctggct gttttccaga atcagcggcc aggaggtctg ctgttcatac
     9721 acctctacag aaccgaccac atgcggcatg atcacctgtg ccgccccggt tgaataatcg
     9781 ccgctatgcc cggaataacc gcccgccagg ttcatataac gttgcagtaa ggtttgcgct
     9841 ttatgcaaca cgccagaaga gcgccagccg tatgatccgg caaaaatagc cgatggtccg
     9901 ttagctttgc gaatgcgatc gtgttgctca tgaatcagtt ttaatgcttg ttcccagctc
     9961 acctgtacat aggtatcttc gccacgacct ttcgtcggtt gcagtggatt atcgagatag
    10021 ctttttctca ccatcggatg ctgaatacgc gtcgtggtgt gtacctgatc cgccgccgta
    10081 gactgtaagg aattcggtat ggttttcgcc agcgcgcctg ttgaagaaac aatcttgccg
    10141 tccttcactt ctacgttcat cgctccccaa cgtcccgcgg tgaggatttt accgtccttc
    10201 tcttctgccc atgcggggag cggtgctgcc gatgtcacca ccagcgctcc agcggcaata
    10261 ccgctgtgtt taataaattc acgtcttgtt aatgtcataa cttcctccct gatcaacgag
    10321 gatcactgtt tctcggtaat atctttggcg ttgtactgga aataccgcgt taaaatgtcc
    10381 agttcgtttt cgctcatgct ggttcgtgcc cccattcctt tggcaatgga cggccacgca
    10441 ttgacggtgt aatggtcggc ggcaataggg gcatgacaac cagcgcaata ggtatcgtca
    10501 agtttttcag cgtattgcca tagcggttta cggtccgcta atccgggatc ggtaagcgca
    10561 ccctgtaaag acgcctgacg ccattgattg ccgtattcgt cagcctgcca ttccccgttt
    10621 accgtgagcg ccttgatacc ttcttcactt aatgtggcta gcgccagccg ttgacctgcc
    10681 gccaggtaga gcgtgttttc actgccctgc atttgataac cctgcaacag aacgatcggc
    10741 tgtttgccac tggcatcaac gacggtgaga tcggtaccag gattcacggt agccagctcg
    10801 cctatgcgag aagttttgaa aggataaata tgtgcgccat tagtcactga agtagcggcc
    10861 tgactttcca gctcatgcgc cgcgttgtca tccattttta tttctggcgg aaaatgggca
    10921 atgcctttat gacaatcgat acaggtttcg ccgccctttt gtgctttgtt atgcattttc
    10981 tgcgcagatt cactttgcga ggcaatatcc atggcatcaa aagaatggca actacggcac
    11041 gttgcagagt cagttgcttt taattctttc catactgttt cggccatttc ctggcgatga
    11101 gtttcgaact tatcgtcact gtctattttg ccgctaacaa attcatgata aatatcttta
    11161 gatgctttta atttagcaaa taaataatcc atccctgact ttggaatatg gcaatctgca
    11221 cattccgcac gtatcccttt ctggttcgaa aagtggacag ttccctgata ttcctcaaaa
    11281 ggtttactca tcgagtggca ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct
    11341 ttttgcgcca gcaataaccc gccgccacca accacaaccg ctatcagcaa aaacaataac
    11401 ccaatgcgtt ttttccctcg catagattcc tctaaataaa ttccagaaca ccgaaatcat
    11461 tgtataatta aatacacaac gaattaaccg cttgcataac tcggcacaac actgcctgaa
    11521 ataatcgata aagaatatga tttattacaa tgtaatcatt aattgctaag gaataaccca
    11581 gttgctattt agaatatata tttatcagtt ttagcaattt aaatcccata atcaatgtga
    11641 atataaaaaa tattattagc acaacgctat gttcactgtc acccgctcaa cagatcacgg
    11701 ttatcgttcg ttttttatac tgctcaggga taaaaaaagg cccctgttga aattgcaggg
    11761 gcctggtacg agcaagcatc atattgggcg acatgatgca acggtaaaaa tcatttggcc
    11821 tgatggcgtt caatgattcc tttcatttca gcaaccgccg ccccgtctac gatataacgc
    11881 gaatactcgt ccgcgtgttc atctgatgac atggacaatc cttgattacg ataacgcatc
    11941 ggtgaggctt gccacgctcc cgcggagcta tggacttcca gcactccggc atcgaggaag
    12001 tggtgcaggt tttctgcacg gactcctgct ccggccataa tgattggagc atcacgatgg
    12061 gcaataagtt ccataatttt tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc
    12121 agtacccggg caatgcccaa ttccgcaaga ttattgagtg tatataaagg gttagcgcac
    12181 atatcgaagg cgcgatgaaa agtcactgcc agcggaccgg cagcagccat tattttttcc
    12241 attcgtggca tatcgacatt cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa
    12301 cctaattcgc ggaccgtgcg cacatcctca agaatggcgg caaactcacc gtcgctgtag
    12361 caaaaatcac caccgcgtgg gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc
    12421 acggatttca gtacccccaa cgacggcgtt aagcccccct cttttggggc tgcgcataat
    12481 tcaactctgt ctgcgccgtt ttgctgcgcc gttagtgcac attccatgct gtaacagcaa
    12541 atttccagta atgccatttt tgctccttaa ttacgctgac tgctcgctgg caacgatctc
    12601 ttcaatggac cacggatgaa acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg
    12661 attcggtaat cgctcatgct caccttttgg tgaactggtt ttaactgaga tccccggtag
    12721 gctgagtcct tcatcagaaa gcaatgccag cgcacgggca ttcagcgttt ctggatcacc
    12781 cagcagaacg atttcaatat gttcccaacc ttcgtgtggg taacgttttt ccccgggcca
    12841 cggtagctcc acaatagaaa actgccagtg cgcaacctgt accggttcat gcaatttaaa
    12901 cagacaaatc ggtctgccat tgatcatatt ttctgacaaa agctcgccac actgttcaaa
    12961 cccgcgacgc cagcgttcag cagtggcgtt ttgatggcaa cgcaaagaaa tgtgatcggc
    13021 agtcagcgga gtgatattca accccagacg gagggaaagt tcatctaatg cgtgggtaaa
    13081 tcgcggtaaa tccgatgcaa tatcctgcag ctcgtcgata gattgccagt tcgccataat
    13141 cactcttcgt ctttcagtaa aagcgttaat ttaccctgtt gccctgcgcc aaccaaccgc
    13201 tgatttcacg ccgcttctga tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct
    13261 gacgaaaaca gccatttgca gtatactccc gccctaattt ctttaactgg tgcgggcaat
    13321 ttttgctcgc ttcatcaatg taaggtattc cggtgaatat tcaggctctt ctctcagaaa
    13381 aagtccgtca ggccatgatt gcggcaggcg cgcctgcgga ttgcgaaccg caggttcgtc
    13441 attcagcaaa agttcagttc ggcgactatc aggctaacgg catgatggca gttgctaaaa
    13501 aactgggtat gacaccgcga caattagcag agcaggtgct gactcatctg gatcttaacg
    13561 gtatcgccag caaagttgag atcgccggtc caggctttat caacattttc cttgatccgg
    13621 cattcctggc tgaacatgtt cagcaggcgc tggcgtccga tcgtctcggt gtttctacgc
    13681 cagaaaaaca gaccattgtg gttgactact ctgcgccaaa cgtggcgaaa gagatgcatg
    13741 tcggtcacct gcgctctacc attattggtg acgcagcagt gcgtactctg gagttcctcg
    13801 gtcacaaagt gattcgcgca aaccacgtcg gcgactgggg cactcagttc ggtatgctga
    13861 ttgcatggct ggaaaagcag cagcaggaaa acgccggtga tgaaatggag ctggctgacc
    13921 ttgaaggttt ctaccgcgat gcgaaaaagc attacgacga agatgaagag tttgccgagc
    13981 gtgcacgtaa ctacgtggta aaactgcaaa gcggtgacga atatttccgc gagatgtggc
    14041 gcaaactggt cgacatcacc atgacgcaga accagatcac ctacgatcgt ctcaacgtga
    14101 cgctgacccg tgatgacgtg atgggcgaaa gcctctacaa cccaatgctg ccaggaattg
    14161 tggcggatct caaagccaaa ggtctggcag tagaaagcga aggggcgacc gtcgtattcc
    14221 ttgatgagtt taaaaacaag gaaggcgaac cgatgggcgt gatcattcag aagaaagatg
    14281 gcggctatct ctacaccacc actgatatcg cctgtgcgaa atatcgttat gaaacactgc
    14341 atgccgatcg cgtgctgtat tacatcgact cccgtcagca tcaacacctg atgcaggcat
    14401 gggcgatcgt ccgtaaagca ggctatgtac cggaatccgt accgctggaa caccacatgt
    14461 tcggcatgat gctgggtaaa gacggcaaac cgttcaaaac ccgcgcgggt ggtacagtga
    14521 aactggccga tctgctggat gaagccctgg aacgtgcacg ccgtctggtg gcagaaaaga
    14581 acccggatat gccagctgac gagctggaaa aactggctaa cgcggttggt attggtgcgg
    14641 tgaaatatgc ggatctctcc aaaaaccgca ccacggacta catcttcgac tgggacaaca
    14701 tgctggcgtt tgagggtaat accgcgccat acatgcagta tgcttacacg cgtgtattgt
    14761 ccgtgttccg taaggcagaa attaacgaag agcaactggc tgcagctccg gtaatcatcc
    14821 gtgaagatcg tgaagcgcaa ctggcagctc gcctgctgca gtttgaagaa accctcaccg
    14881 tggttgcccg tgaaggcacg ccgcatgtga tgtgtgctta cctgtacgat ctggctggtc
    14941 tgttctctgg cttctacgag cactgcccga tcctcagcgc agaaaacgaa gaagtgcgta
    15001 acagccgtct gaaactggca caactgacag cgaagacgct aaagctgggt ctggatacgc
    15061 tgggtattga gacagtagag cgtatgtaat cgatttttcg tgagagtaaa gcctgatcag
    15121 ggttacctga tcaggctttt ttattgccat ctaaatgtat tctgaaaatg gacatgccat
    15181 tgttttctga ctgttggata agaggccaga agcgtaatat ccggccccag ggaaacgata
    15241 acggttgaat ttaaggaata ccgcagtgtt taaatttctt gtattaacat taggcattat
    15301 ctcttgccag gcttacgcag aagatacggt tatagaaaac gaccatgaca tttcagccat
    15361 caaagattgt tggcaaaaaa attcagatga tgatactgac attaacgtga tcaaatcatg
    15421 cctgcgacaa gaatacaatc tcgtcgatgc gcaattaaat aaagcctatg gtgaagctta
    15481 tcgttatata gaacaagtgc cacgcacagg tgtaaaaaaa cctgataccg aacaacttaa
    15541 cttgcttaaa aaatcacagc gagcctggct ggattttagg gacaaagaat gtgaattaat
    15601 cctttcaaat gaggacgttc aggatttaag tgacccttat tctgaatcag aatggctctc
    15661 atgtatgatc atacagacca atacgcgaac tcgccagttg cagctatacc gtaactctga
    15721 agatttttat ccaagccctt tgacaagagg ataattcaca tctttttggc atgttttgtt
    15781 gcaagctatt cctgataaat aattgcaaca agacatcgag cctttttcac tgagttatta
    15841 aacatactcg cgagcgcgta atttttttgt ccttcagcga taattcacaa tcacttcatt
    15901 acgttgtacc tttagcggtg gaattaaccg cccaccgcct ggcacttccc agataaatcg
    15961 caacggttct gctgccgcta taccggaaaa ggccacggtg gttccgctct gcccctctaa
    16021 ttcgacacaa cgagattgcg aacacaagcg aacccgcagt cctgaaggtg tcgggccgat
    16081 aagctgataa cgccacgcta ccagcgtcat caatcctgaa gcaggttgtc gcgtagaaag
    16141 aggcgcagac gacatcgact caccgcgatg atttagcgta attcctacac tacttgcctg
    16201 ccacatcccc tccccggcgg cttgcaccag cagcggaaac aataatattg ctaataaggt
    16261 tctcattatt tgccgccaat tgtcgccgtc atgcggatat gtcggttatc agacagttcc
    16321 agattcgaca ggaccactaa ctgcggcaag ctgcggcgca ggaagcgaga caataatggt
    16381 cgcagcgcgt ggttcaccaa caatactggc ggcgcgccca gcatctcctg acgggatagc
    16441 gcttcctgag tttgcgccag taaacgatcc gccagccctg gctccagtcc tcccccgccc
    16501 tgcagcgcct gtagtaacaa acgttccagc ggtgtatcga ggccaataac atggacttca
    16561 tctttgccag gaaaccactg ctgggtaatc gcccgtccca acgccacgcg cacgacggcg
    16621 gttaattcat gtggatcgct ttggatgggc gcatgttccg ccagcgtttc gagaatggtg
    16681 cgcatatcgc gaatcggtac tttttcatcg aggagatttt gcagcacttt atgcagtgtg
    16741 gtgagcgtga cgacgccagg aacgagatct tccgtcagct ttggcatctc ctgggcgacg
    16801 cgatccaaca gctgttgcgc ctcctgacga ccaaacagct ctgcggcatg ctggctaatg
    16861 aggtggttaa gatgcgttgc taccaccgtg ctggcctcaa ccactgtgta cccctgaatc
    16921 tgcgcctgtt cttttagcgc actttcaatc cagatagcat tcaggccaaa tgccggatcg
    16981 acggtcgcct caccaggtaa cgtcccggca gcggttccag ggttaatcgc cagccagcgc
    17041 cccggataag catcaccact gccaatctcc acgcctttca tcaaaatgcg atagcgggca
    17101 ggttgcagat ccatattgtc gcgaatgtgc accactggcg gcagaaatcc catctcctgg
    17161 gcaaatttct tgcggatact gcgtatacgg cccaacaact caccatcctg ctggaaatcg
    17221 accatcggga tcagtcgata acccacttcc attcccagag aatcttccag ttgtacatcg
    17281 ttccacgtcg cttcgacaac ggtattattc tctgccattt ttaccggttt gggttcggca
    17341 ggcgcttttt gttcgcgtcc gcgtatccac caggccagcc cgagcaatcc ggcagtgaac
    17401 agcaaaaata ccaggttcgg cattccaggc accaggccga gtaaaccgag cacggcggcg
    17461 cttaacaaca taacgcttgg gttactgaaa agctgattca ccatctgctc gccaacatcc
    17521 tgatcggtgc tgacacgcgt aacgatgacc cccgcggcgg tagaaatcac cagcgccgga
    17581 atttgtgcca ccagaccgtc gccaatggtc aatagcgtat aactttccgc cgcgtgtccc
    17641 atgctcatgc catgttgcag cacgccgacc agcaacccgc cgacaacgtt aatgaccatg
    17701 atgaggatcc cggcgatggc atcgccgcga acaaacttac ttgccccgtc cattgagccg
    17761 taaaaatcgg cttcctgagt cacttcggag cggcgttttt tcgcctcatc ttcaccaatc
    17821 aatccagcgt taaggtcggc gtcaatcgcc atctgcttac ccggcatacc atcgagaaca
    17881 aagcgcgcac ccacttctgc gatacgcccg gcacctttgg taatgaccat aaagttgatg
    17941 atcacgagaa tgacaaacac cacgataccg atagcgaaat tgccaccaac gaggaagtga
    18001 ccgaacgctt cgaccacctt ccctgccgcc gccgcgccgg tatgcccttc catcaaaatg
    18061 atacgggttg aagccacgtt aagtgccaga cgcaacaacg tagtaaacaa cagaatggtc
    18121 ggaaacgcag caaactcaag cgtgcgctgg gtaaacatcg ccaccagcaa caccatgatc
    18181 gacaaggcaa tattgaaggt aaacaacagg tcgagtatga atgcgggcag tggcagcacc
    18241 atcatcgaca agatcaacag gatcaaaatc ggtccggcaa ggatctgcca ttgtgtcgat
    18301 ttcaggtttg cgggcaggcg cagcatcgcg gccagattac tcatgggtcg gtttctcgtt
    18361 aataaaatcc agggcttccg gcaccggaag atgagtaggt tgtacagggc gctgtccacc
    18421 agccagacgc cagcgtttca gttgccagac ccaggccagc acttccgcca ccgcggcgta
    18481 cagttgaccc gggatttgtt gaccaatctc cgcatgtcga tacagcgctc gcgccagcgg
    18541 cggcgcttca agcgtcggga cgttattttc agcgccaatt tcacgaatgc gcagcgcgac
    18601 cagccctgca cctttagcga ccactttcgg tgcgctcatt ttgttttcgt catactgcaa
    18661 cgctaccgaa tagtgggtcg ggttattgac aatgacatca gctttcggca catcggccat
    18721 catccgacgc cgtgcagcag ctcgctgcat ctgacggatc cgccctttaa catgagggtc
    18781 accttcgctt tgtttgaact catcacgaat atcctgccgc gacatacgca gctttttcag
    18841 gtggctgaag atttggaaaa agacgtcaaa tcccaccatt ggaatgacac caagcaccac
    18901 cagcagtgcg catagcccaa ccagatccat cgcattaccc atggcggtag tcggagactc
    18961 ggccattaag cgcatcatct gcggccagtg atgccagaga taaaaccccg tcacgctgcc
    19021 aaccaggatg gttttcaaaa ttgccttaag caactccgcg ccagtctgtg ccgagaacat
    19081 ccgtttaatg cccggtagcg ggttgagttt ggaaaacttc ggctgcaagg atttgccgct
    19141 aaataccagc ccccccagca tgaccggaga aatgagcgcc accagcacca cgccgctaat
    19201 cagcggcagc agcgccagca tggcttctct gatcagcaga ataatctgcc cgaggatcag
    19261 attcgggtca
//
LOCUS       DAZPLI010000073        18842 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZPLI010000073 DAZPLI010000000
VERSION     DAZPLI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18842
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.074"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..101)
                     /locus_tag="ACHUU3_002433"
     CDS             complement(<1..101)
                     /locus_tag="ACHUU3_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2082465.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTA"
     gene            complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="ACHUU3_002434"
     CDS             complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="ACHUU3_002434"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR2082466.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2315..2605)
                     /gene="hupB"
                     /locus_tag="ACHUU3_002435"
     CDS             complement(2315..2605)
                     /gene="hupB"
                     /locus_tag="ACHUU3_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR2082467.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2796..5150)
                     /gene="lon"
                     /locus_tag="ACHUU3_002436"
     CDS             complement(2796..5150)
                     /gene="lon"
                     /locus_tag="ACHUU3_002436"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR2082468.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="ACHUU3_002437"
     CDS             complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="ACHUU3_002437"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR2082469.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="ACHUU3_002438"
     CDS             complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="ACHUU3_002438"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR2082470.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7607..8905)
                     /gene="tig"
                     /locus_tag="ACHUU3_002439"
     CDS             complement(7607..8905)
                     /gene="tig"
                     /locus_tag="ACHUU3_002439"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR2082471.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKRALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="ACHUU3_002440"
     CDS             complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="ACHUU3_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR2082472.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGVQDTVFASPPCRGAG
                     SIA"
     gene            9871..10449
                     /locus_tag="ACHUU3_002441"
     CDS             9871..10449
                     /locus_tag="ACHUU3_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR2082473.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10493..11968
                     /gene="ampG"
                     /locus_tag="ACHUU3_002442"
     CDS             10493..11968
                     /gene="ampG"
                     /locus_tag="ACHUU3_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR2082474.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12429..13376
                     /gene="cyoA"
                     /locus_tag="ACHUU3_002443"
     CDS             12429..13376
                     /gene="cyoA"
                     /locus_tag="ACHUU3_002443"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFR2082475.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13398..15389
                     /gene="cyoB"
                     /locus_tag="ACHUU3_002444"
     CDS             13398..15389
                     /gene="cyoB"
                     /locus_tag="ACHUU3_002444"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFR2082476.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15379..15993
                     /locus_tag="ACHUU3_002445"
     CDS             15379..15993
                     /locus_tag="ACHUU3_002445"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR2082477.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15993..16322
                     /locus_tag="ACHUU3_002446"
     CDS             15993..16322
                     /locus_tag="ACHUU3_002446"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR2082478.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16334..17224
                     /gene="cyoE"
                     /locus_tag="ACHUU3_002447"
     CDS             16334..17224
                     /gene="cyoE"
                     /locus_tag="ACHUU3_002447"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR2082479.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17409..17720
                     /locus_tag="ACHUU3_002448"
     CDS             17409..17720
                     /locus_tag="ACHUU3_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR2082480.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17704..18246
                     /locus_tag="ACHUU3_002449"
     CDS             17704..18246
                     /locus_tag="ACHUU3_002449"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR2082481.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNSVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18302..>18842)
                     /locus_tag="ACHUU3_002450"
     CDS             complement(18302..>18842)
                     /locus_tag="ACHUU3_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR2082482.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4213 a         4956 c         4581 g         5092 t
ORIGIN      
        1 gccgtcggtt tcgccacaga agctgccgcc agcgcgctat gagacattcc ggcacaggcc
       61 agggacaggg taatgagcag tgctttaatt ccgtgtttca tactgttttc tccttgtttg
      121 ttaacagtgg aatcacctta acgggagtga aaacagtaaa caaatggcaa tgttcagaaa
      181 tggaaaaggc cgcgaaagcg accttttctt gttacagctg caacgcattt ttttgcgagg
      241 cggcttcagg attattgctg ttccagcgca tcgccaattt tgattttcgc ctctttacgc
      301 aggttactca tcagagcttc aaagacgatt tgtgcgttgt tctgggtgat accctgcacc
      361 atcgcttttt tctgatcttc cggcattgaa ccttgtttca cttcatccag cgccagcaga
      421 accacattac cttgcatatc ggtcgccata ccgtagctcg gtttgtcttt cgctggcagt
      481 ggcagtgcaa acgccgcctg gctaatcggg tcacgaccgg aacggcttaa ggttttcggc
      541 tcgccaaatt tcagaccggc agcctgcata gcttccgccc ctttgccggc tttcaaatca
      601 accagcagtt tctcagcatc cactttcgcc tgttgttcag ctttgttgtg ctgaaccagt
      661 gccttaactt gttcctgaac atctgccaac ggtttcaccg cttccggttt gtgttcgctg
      721 atgcgcagca cgaatgcgcg gtcgccgtct acggtgatga tgtcagagtt gatgcccggc
      781 gcgccgtttt cacctaccag accgccgtta aagatagcgt cggcaaccgg cttgaagttc
      841 aactcttccg gcaggttatc tttgctgaac caacccgtct gagtggcttt aacgccggca
      901 gcttgctctg caccggccag agactcggtg tcgttgcttg ccgcatcgct cactttctgc
      961 tgcagcgcat agtacgcatc gagggctttt tcgtgtttca ctttcgccgc aatgtcgtca
     1021 cgtacttcgt ctaacgattt cactttcgct ggctgaatgt cgtccagacg tacaatcagg
     1081 aaaccgaccg aagatttgat gacaccagac agttggcctt tttctttcag accagcattt
     1141 ttcagttcgt cggggatagt ggcatcttct aaccaaccca tatcgccgcc gttacgggca
     1201 gagataatat cggcagattt ttctttggct aatgcagcaa aatcaccgcc tttattcagc
     1261 tcatcaagta ccgctttcgc ttcatcttca gttttggtct ggatgatgct gtagcgggta
     1321 cgctgcggct gggtgaattg atcctgatgc tgatcgtagt agctctggat atccgcatcg
     1381 ctaaccggtt gctgcatcgt tgcggcatcc agcttgatgt aactcacgcg gaattgttcc
     1441 ggcgtcatga aattgttttt gttttgttcg tagtagctgg caatctcctg ttcggtcaca
     1501 ggctgcttcg ccgccagcgc gttaacatcg atagtcgcct cacgcaccac gcgttgttgc
     1561 gcgaccagtg ccgccagctc gtcggtttca cctttcagca taaaatcggt accggcaaca
     1621 ccgttaatca gctgttgggt agtgagctgg ttacgcagcg cctgggcgta ctgatcggcg
     1681 gtcatcccca tctggttgag gataccgtta tagcggctgt tatcaaattt gccgtcaacc
     1741 tggaaggctg gggtcgcgaa aatcgcctgt ttaacctgct catcactgat acccagtttc
     1801 agctcacgag cgtactgatc caacagcgcc tcgtcgatca gacgattcag cacctgttga
     1861 cgcagggttt tcatatagcc ttcgttcgct gccagctcgg agtattgatc gcccagctgt
     1921 tgctgcatgc gattacgctc gctgttaaag gcgttctcga actgcccacg gctgatttcc
     1981 tggtcattca cttttgcggc gtagttattg cctccgccaa tcaggtaacc actcacgccg
     2041 gtcaatatga acgacacgat aatgataccg aaaataatct tgagcacgag actgtttgca
     2101 gccgtgcgta agctgtccat catggtgtaa caacactccg ctgtagatga ctgtgtacct
     2161 cacgcaacgt atcgatgcgc gcaaaggcct attgtgacaa gaaacggggg caattgtcag
     2221 cccacaagac acagaaagtc accgaataca aataaaaaag gcacatcagt agatgcgccc
     2281 ttgaacttcg tcacatcccc actggggaca acgcttagtt taccgcgtct ttcagtgctt
     2341 tacctgcacg gaagctcggt actttagcgg cagcgatggt gatctcttta ccggtctgcg
     2401 ggttgcggcc agtacgggca gcacgctctt taacggcaaa agtaccaaaa cctaccagtg
     2461 ctacatcatc cccttctttc agagattcag ttacggaagc aataatagca tctaacgcac
     2521 ggccagccgc agctttagag atatcagccc ctgcagcaat cttgtcgatc aattgagatt
     2581 tattcactct tctcttcctc tttataattt atatcgcact tgaatccttc aaggtacgaa
     2641 cgcgcagcag ttatatcagg ccagccatcc ccttacaaca ccccttaata agggcaagcc
     2701 cgatccgcca tctaacttag cgagacaaaa aaaggctggc aagcccgaat tagcctgcca
     2761 gccctgtttt tattagtgca ttttgcgcga ggtcactatt ttgcagtcac aacctgcatg
     2821 ccagacggtt cattttgcag cgccagagtc agaacttcct caatgcgctt cacaggatga
     2881 atgtccagat cggcaattac gttgtcagga atctcttcca gatcgcgttt attttcgaac
     2941 ggaattagca ctgttttaat cccgccgcga tgcgctgcca ggagtttttc tttcaaacca
     3001 ccgatcggca gtacctgacc acgcagagtg atctcaccgg tcattgccac atcggcacga
     3061 accgggttac cggtcaggca agaaaccagc gcggtgcaca tagcaatacc ggcactcgga
     3121 ccatctttcg gcgtcgcacc ttccggtacg tggacgtgga tgtcacgttt ttcgtaaaaa
     3181 tcagggttga tccccagttt ttccgcacgc gcacgaacca ccgttaacgc cgcctgaata
     3241 gactcctgca tcacttcgcc gagcgaaccg gtataggtca gtttgccttt acccggaaca
     3301 catgcggttt caatggtcag caagtcaccg cccacttccg tccacgccag accggttacc
     3361 tgaccgacac ggttttcgtt atcagcgcga ccatagtcga aacgctgaac accgaggtag
     3421 tcatgcaggt tatcgccgtt aatttcgata tgttttaatg acttatcgag cagtaactgc
     3481 ttaaccgctt tgcggcacag tttggagatt tcacgctcca gaccacgcac gcccgcctca
     3541 cgggtgtagt aacgaataat gccgataatg gcgctatcgt cgacggtcag ctcacctttt
     3601 ttcagtgcat tacgttcaat ctgcttcggc agcaggtgac gtttggcgat gttcagtttt
     3661 tcatcttcgg tataaccgga gaggcgaatc acttccatac gatccagcag cggtgccgga
     3721 atgttcatgg agttcgacgt cgcgacaaac atcacgtcgc tgagatcgta atccacttcc
     3781 aggtagtggt cgctgaacgc tacgttctgc tctggatcca gcacttcaag cagtgcagag
     3841 gccggatcgc ctcgcatgtc agaagacatt ttgtcgatct catcgagcag gaacagcggg
     3901 tttttcacgc ccactttcgc cattttctgg atcagtttac ccggcataga accgatgtaa
     3961 gtacggcggt gaccacggat ttccgcttca tcacgcacgc cgcccagcgc catacggaca
     4021 tatttacgcc cggtggcttt ggcaatggac tgaccaagag aggttttacc tacccccggc
     4081 ggccctacca ggcagaggat cggtcccttg attttgttga cacggctttg aaccgcaagg
     4141 tactcaagga tgcgatcttt cacgcgctcc agaccataat ggtcggtatc aaggatttcc
     4201 tgcgcctgac gcaggtcttt tttgaccttg ctgcgcgcat tccacggtac ctgtaccatc
     4261 cagtcgatat aaccacgcac tacggtcgct tctgccgaca tcggagacat cattttcagc
     4321 ttctgcaact ctgcttccgc tttctctttt gcctctttcg gcatcttcgc cgcgtcgatt
     4381 ttgcgcttca gggcttcgtt ttcgtccggc gcgtcgtcca tttcaccgag ttctttctga
     4441 atagctttca tttgctcgtt cagatagtac tcacgctggg atttctccat ctgcttttta
     4501 acgcggttgc gaatgcgttt ctcaacctgc agcagatcga tttccgattc catcattgcc
     4561 atcagatatt ccagacgttc gttaacgtcg gacatctcca gaacggactg tttgtcagcc
     4621 agtttcagcg gcatatgtgc agcaatggta tccgccagac gcgccggatc gtcgatgcta
     4681 ttcagcgacg tcagcacttc tggtgggatt tttttgttca gcttgatgta gccttcgaac
     4741 tggctgattg cagtacgcac cagcacttcc tgttcccgct catcaatggt cggcgactcc
     4801 agatactccg ccttcgcaga aaagtgttcg ccattgtcag agagcgcaga aatacgcgcg
     4861 cgctgtaacc cctcgaccag cactttgacg gtgccgtcag gcagtttcag catctgcaat
     4921 atagaggcca cggtcccgac ggtgaaaaga tcgtttacac ccggctcatc cgttgaagct
     4981 tctttctgcg cgaccagcat aattttttta tcatggtcca tcgccgcttc cagacaacgg
     5041 atagattttt cccgcccgac aaataagggg atgaccatgt gcggataaac caccacatcg
     5101 cgcagcggca atacggggat ttcaatgcgt tcagaacgct caggattcat agagctctct
     5161 cttagtttaa tttccgccag gtaatcagat gacacgactg tgcttcacgc catctattaa
     5221 catgtacgtc agtatatggg gatgtttccc ccacattcaa cgccgagaat agaggaaaaa
     5281 ttaaagggga gataaaatcc cccctttttg gttaactaat tgtatgggaa tggttaatta
     5341 ttcaccagat gcctgttgcg cttccggctt accataaatc agcaacggtt tgctttgacc
     5401 atcaattacc gactcgtcga taaccacttt ttcgacgtct tccatggacg gcagatcgta
     5461 catggtatcg agcagtgcgg cttctacgat ggaacgcagg ccacgggcac cggttttacg
     5521 cgccatcgct ttcttagcga tagcatccag cgcctcgtca cggaattcca gatccacgcc
     5581 ttccagatta aacagcgcct gatactgctt ggtcagggcg tttttcggct ctttgaggat
     5641 ctgaatcaga gcttcttcgc tcagttcatt caacgttgct acaaccggca gacgaccaat
     5701 gaactcaggg ataagaccaa acttgatcag atcttccggt tcaacctgcg ccagcagctc
     5761 gccttcgctt gctttgtcgg acttcgcttt taccgtcgcg ccaaaaccaa tgccggagcc
     5821 ggtttctaca cggtgggaaa tcactttatc cagaccggca aacgcaccgc cacaaataaa
     5881 caggatctta gaggtatcaa cctgcaagaa ttcctgctgc ggatgtttac gcccaccttg
     5941 cggtggaaca gcagctaccg taccttcgat cagtttcaac agtgcctgct gtacgccttc
     6001 accggaaacg tctcgggtaa tggacgggtt gtctgactta cgagaaatct tgtcgatttc
     6061 atcgatgtag acaatcccgc gctgcgcttt ctgtacgtcg taatcgcact tttgcaacag
     6121 cttctgaatg atgttttcaa cgtcttcgcc cacataaccg gcttcggtca gcgtggttgc
     6181 gtcggccatg gtgaacggga cgtccagcag gcgcgccagc gtttcagcca gcagcgtttt
     6241 accggaaccg gtcggaccga tcagcagaat gttacttttg cccaactcga caccattgct
     6301 ggtatcgccg ttgcgcagac gtttgtaatg gttgtatacc gcgaccgcca gcactttttt
     6361 cgcctgttcc tggccgataa cgtaatcgtc caggtggttg cgaatttcat gcggcgtcgg
     6421 tagcgcactg cgttcacgat gcggtgcaac ttctttaatc tcttcgcgaa tgatgtcgtt
     6481 acataaatca acacattcgt cgcagatata cacggatgga ccggcaatca gcttgcgcac
     6541 ttcatgctgg cttttgccgc aaaaagagca atacagcaat ttgcctgagc catctttgcg
     6601 tttatctgtc atgggtcaaa acctcttctt tgttctttgt gccgcacacg acgacgcaaa
     6661 tgccattctc aggcgcaagc cgcttacagc gttgtgccgc cctggataag tatagcggca
     6721 cagttgcgcc tctggcatca attacgatgg gtcagaatcg aatcgaccag accgtattcc
     6781 accgcttcag gggcggaaag gaagcgatcg cgctcggtat cacgttcaat ctgttctaat
     6841 gattgacccg tatgaagcgc cataagttca ttcatgcgcc ctttaacttt cagaatttca
     6901 cgggcatgaa tttcgatatc ggtcgcctgg ccctggtagc cgcccaacgg ttggtgaatc
     6961 atcacgcgcg aattcggcag gcaaaaacgt ttaccttttg cccctgcggt cagcaagaaa
     7021 gcgcccatcg aggccgcctg gcccatacag atggtgctga catcaggctt gataaactgc
     7081 atggtgtcat agatagacat cccggcagtg atcaccccgc ctggggagtt aatgtacaga
     7141 tagatatctt tttctgggtt ttccgcttct aggaacagca tctgcgccac aatcaggttc
     7201 gccatgtggt cttcaacctg gccagtcaga aaaatgacgc gttccttaag tagacgagaa
     7261 tagatatcaa aagagcgctc accgcgtgag gtctgttcaa tgaccatcgg caccagcgcc
     7321 atatggggtg caaagttatc tcgttcgccg ctgtatgaca tttccgtctc ctggataaaa
     7381 ttgaaaaaac ctgctgtacc gattgtaacc ttatggacgg attatcagct agtccctgtg
     7441 ttatgggtac ggcatcacca tatttcaagc ataacaatct tttgttgtta cgctaacacc
     7501 gaaagaggct tttcgcacgg ttccatgcaa aattcatgac aaaaaaaagc ccaccacctg
     7561 gcggtgacgg gcctttgtgc gaatttagcg cgttatgctg cgtaaattac gcctgctggt
     7621 tcatcagctc gttgaaagtg gtttcttttt cagtcacttt cgctttcgcc agtacagctt
     7681 caacagcctg ttcttccaga gcaacattgc gcatgttgtc catcagttct ttgtttttgc
     7741 tgtagaactc gataacttct ttcggatctt cgtacgcaga agccatctct tcgatcaggc
     7801 ctttcacgcg ctcttcgtca gctttcagct cgttggtgcg gataacttcg cccagcagca
     7861 ggccaacaac tacgcggcgt ttagcctgtt cttcgaacag ttcgcgcggc agttccagag
     7921 ctcgtttttc gttgccaccg aaacgctgtg cagcctggcg acgcagaacg tcgatttcgc
     7981 tgtcgatcag cgcagccggt acgtcgatgt cgttagcttt taccagacct tcgatcgcct
     8041 gagacttaac gcggttacgg atggcgctct tcagctcgcg ctccatgttt ttacgcactt
     8101 cagcgcgcag accttctacg gaaccatctt caacgccgaa acgtttgatg aattctgcag
     8161 tcagttccgg cagttcacgc tcttcaactt tcttcaggtt gatagcgaat ttcgctgctt
     8221 tacctttcag attttctgcg tggtattctt ccgggaaggt cacgtcgatg gtgaactctt
     8281 cgccagcttt gtggcctttg ataccgtctt caaagcccgg gatcatacga ccctggccca
     8341 tcgccagtac gaaatcagac gctttaccgc cttcgaactc ttcgccgtct acagaaccgg
     8401 tgaagtcgat ggttacgcgg tcttctgctt caacggcgcc gtctttttct ttccaggtcg
     8461 cctgctgttt acgcagagta tccagcatgc cgtcaacgtc agcgtcggtc acttcaacga
     8521 tcggtttttc aacttcgatc gcttccagac cctgcagttc aacttccgga taaacttcaa
     8581 actctacaga gtaggtgaag tcttcaccca gcttgtattc gcccggaaca taagtcggtg
     8641 cgccagccgg attgattttt tctttaatga tggcgtcaat gaagttacgg ctcatcaggt
     8701 cacccagaac gtcctggcgt acagacgcgc cataacgctg agcaacgata ttcattggca
     8761 ctttgccttt gcggaagccg tcaatacgta cttttttcgc aacgttgacc agctcgcttt
     8821 taacagcggt ctcgatgctg tcagcagcga tagtaatcgt tacacggcgg ccaaggcctt
     8881 gagtggtttc aactgaaact tgcatcttgt tacctcaaaa aatcacagtg ctcggtcaac
     8941 tcttaccccg caagcacagg ttgttggctt cgcggaaccg ggatgttctt ataatcaatc
     9001 acattccctg ttaccagaat catcccgaaa gcattcaaat aggacgcggc attatagcgg
     9061 catcactttt atgagtcgag aacggttatt gcgcgctgtc gcgtattttt tcacaattcc
     9121 cgggcaattt tcgtctaaat gctgggcaaa aagcgcaaaa tccagacaaa acaaaacggc
     9181 ccgcaggccg tttttcataa aatctttagc aacatactgg aaaagcgccg acagttgcaa
     9241 atgcgttttt acgcgatgct tcctgctcca cgacagggag gagaggcaaa gacggtgtcc
     9301 tgcacccctt cccactcctt aatagtgtaa gtatgcagag ccagcgcatg aacggtagta
     9361 gagagttcct ccgctaaagt actgtaaatc attcgatgac gattcagaaa acgttcaccc
     9421 gtaaaacgat cgctgaccag cacaacttta aaatggcttt cagagccggc tgggacattg
     9481 tgacgatagc tttcatccac tacttcgagg aatacgggtt ggaacgccgc ccttaatttt
     9541 tcttctatcc gctcacgtat catcatgaaa tatctcctcc gacaacgctg agatgtcatc
     9601 catcccttta aatactagcc gcttttaccg tttccattgc agcttaacaa caaatattta
     9661 ggtttcgtcc tggtttttcc gtcaagcgta tgaagtttac tgatttaaag attgaaacac
     9721 aggcctcgca tcagcgtttc ttttaccagg ggtgattatt tcgccaacat gatgaattta
     9781 catgccttac ccacttcccc tcgccgttgg caatgttatg atggcgggaa tttttaccaa
     9841 acacgcttaa gagtctctga gagcccgaac atgttcaaaa aaatcctctt cccgttagtt
     9901 gctctgttta tgcttgcagg atgcgcaaaa ccgccaacaa ctattgaagt ttccccgacg
     9961 attacactgc cacagcagga tccaagcctg atgggcgtca ccgtaagcat taatggtgcc
    10021 gatcagcgta ccgatcaggc gctggcaaaa gtcacccgcg ataatcaaat cgttaccctg
    10081 accgcctccc gagatctgcg tttcctgctg caagaagtgc tggaaaaaca gatgaccgcg
    10141 cgtggttaca tggttggtcc gaatggcccg gttaatctgc aaatcatcgt tagccaactg
    10201 tatgctgacg tgtcccaggg caacgtgcgc tacaacatcg cgaccaaagc ggatatcgcc
    10261 atcatcgcta ccgcgcagaa tggcaacaaa atgaccaaaa actatcgtgc cagctacaac
    10321 gttgaaggtg cgttccaggc ctccaacaaa aatatcgccg atgcggttaa cagtgtgctg
    10381 accgatacca tcgctgatat gtctcaggac accagcatcc acgaattcat caagcagaac
    10441 gcgcgttaat ttctgccctc tggcccggtg caaaccgggc ctgtagacgc ccatgtccag
    10501 tcaatattta cgtatttttc aacagccgcg ttcagccata ttgctgatcc tgggttttgc
    10561 ttccgggcta ccgctcgccc tgacatccgg taccttacag gcctggatga cggtcgagaa
    10621 tattgatctc aaaaccattg gtttcttctc tctggtaggc caggcttacg tttttaaatt
    10681 cctctggtca ccgctgatgg accgctacac gcccccattt tttgggcggc ggcgcggttg
    10741 gctgctcgcc acgcaaatcc tgttattagt cgccattgcg gcgatgggtt ttctcgaacc
    10801 tggcacccaa ctccgctgga tggcggcact ggcggtggtg atcgcttttt gctcggcctc
    10861 ccaggatatt gtcttcgatg cgtggaaaac cgatgtgctc ccggcagaag aacgtggtgc
    10921 gggcgcggca atcagcgtgc tgggttaccg tttagggatg ctggtttccg gcggcttagc
    10981 cctgtggctg gcagataaat ggctgggctg gcagggcatg tactggttga tggcggcgct
    11041 gttgatccct tgtattatcg cgacgttgct tgcaccagaa ccgaccgaca ccattcctgt
    11101 gccaaaaacg ctggaacaag cggttgttgc acctctgcga gatttctttg gtcgcaataa
    11161 tgcctggctt attttgcttc ttatcgtgct gtataagctg ggcgacgcat tcgccatgag
    11221 cctgacaacc acgtttttga ttcgcggcgt cgggtttgat gcgggtgaag taggcgtggt
    11281 taacaaaacg cttggcttac tggcgaccat tgtgggcgca ttgtacggtg ggattttgat
    11341 gcagcgcctg tcgctgttcc gggcgctgct gattttcggc attttacaag gtgcgtctaa
    11401 cgctggttac tggctgctgt cgattactga taagcatctc tacagcatgg gcgcagccgt
    11461 ctttttcgaa aacctctgtg gcgggatggg cacatcagcc tttgtcgcgc tgttaatgac
    11521 gctgtgtaat aagtcatttt ccgctactca atttgccctg ctctcagcgc tttccgctgt
    11581 aggacgagtt tatgtcggcc ccgtggcggg ttggtttgtt gaagcacacg gctggtcgac
    11641 attctatcta ttctccgtcg ccgctgccgt accggggctt attttgctgc tggtttgccg
    11701 ccagacgctt gaatatacac gagtaaatga caactttatc tcccgtaccg aatatccgac
    11761 aggttatgcc tttgccatgt ggacactggc ggcgggcgtc agcctgttgg ccgtgtggtt
    11821 actgctgttg acgatggacg cgctggattt gacgcacttc tctttcctgc ctgctctgct
    11881 ggaagtcggg gttttagtcg ccctttctgg cgtcgtgctt ggtggtttgc tggattatct
    11941 ggctctacga aaaacgcatc tgacgtaatc tgtaaatatt atttagctgt agtaatcact
    12001 cgccgtaatt attacggcta aataaacatc agtatcgctt aataatattt gttcttttgt
    12061 gcggcttagc gtttggaaat tattgacgca atttatgaat tctatttttt cttacacgat
    12121 tcagctaatg agtctttatt ttccccccac ccaattgtca ccataatgat aattatttgt
    12181 taaacaattg ttttattcca gattggttat accaattgcc cgtccaaact ccttctgcac
    12241 tcccctttcg ttataacgcc cttttgcaac agcttcttaa aatcaacttg atatgttttg
    12301 caacatatgt aacctgacag ccaaatccaa gtaacaggaa tttaatcatg tttacagtaa
    12361 tgtaacctcc ccgtaaaatg cccacacact ttaaacgcca ccagatcccg tggaattgag
    12421 gtcgttaaat gagactcagg aaatacaata aaagtttggg atggttgtca ttatttgcag
    12481 gcactgtatt gctcagtggc tgtaattctg cgctgttaga tcccaaagga cagattggtc
    12541 tggagcaacg ttcactgata ctgacggcat ttggcctgat gttgattgtc gttattcccg
    12601 caatcttgat ggctgttggt ttcgcctgga agtatcgtgc gagcaataaa gatgctaagt
    12661 acagcccgaa ctggtcacac tccaataaag tggaagctgt ggtctggacg gtacctatct
    12721 taatcatcat cttccttgcg gtactgacct ggaaaaccac tcacgctctt gagcctagca
    12781 agccgctggc acacgacgag aagcccatta ccatcgaagt ggtttccatg gactggaaat
    12841 ggttcttcat ctacccggaa cagggcattg caaccgtgaa tgaaatcgct ttcccggcga
    12901 acactccggt gtacttcaaa gtgacctcca actccgtgat gaactccttc ttcattccgc
    12961 gtctgggtag ccagatttat gccatggccg gtatgcagac tcgcctgcat ctgatcgcca
    13021 acgaacccgg tacttatgac ggtatctccg ccagctacag cgggccgggc ttctcaggca
    13081 tgaagttcaa agctattgca acaccggatc gcgccgaatt cgaccaatgg gtcgcaaaag
    13141 cgaaacagtc gccgaacacc atgtctgaca tggctgcgtt cgaaaaactg gccgcgccta
    13201 gcgaatacaa ccaggtggaa tatttctcca acgtgaaacc agacttgttt gccgatgtga
    13261 ttaacaagtt tatggctcac ggtaagagca tggacatgac ccagccagaa ggtgagcaca
    13321 gtgcacacga aggtatggaa ggcatggaca tgagccacgc ggaatccgcc cattaaaggg
    13381 gttgaggaag aataaagatg ttcggaaaat tatcacttga tgcagtcccg ttccatgaac
    13441 ctatcgtcat ggttacgatc gctggcatta ttttgggagg tctggcgctc gttggcctga
    13501 tcacttactt cggtaagtgg acctacctgt ggaaagagtg gctgacctcc gtcgaccata
    13561 aacgcctcgg tatcatgtat atcatcgtgg cgattgtgat gttgctgcgt ggttttgctg
    13621 acgccattat gatgcgtagc cagcaggctc ttgcctcggc gggcgaagcg ggcttcctgc
    13681 cacctcacca ctacgatcag atcttcaccg cgcacggcgt gattatgatc ttcttcgtgg
    13741 cgatgccttt cgttatcggt ctgatgaacc tggtggttcc gctgcagatc ggcgcgcgtg
    13801 acgttgcgtt cccgttcctc aacaacttaa gcttctggtt tactgttgtt ggtgtgattc
    13861 tggttaacgt ttctctcggc gtgggcgaat ttgcgcagac cggctggctg gcctatccac
    13921 cgctatcggg aatagagtac agtccgggag tcggtgtcga ttactggata tggagtctcc
    13981 agctatccgg tataggtacg acgcttaccg gtatcaactt cttcgttacc attctgaaga
    14041 tgcgcgcacc gggcatgacc atgttcaaga tgccagtatt tacctgggca tcactgtgcg
    14101 cgaacgtact gattattgct tccttcccaa ttctgacggt taccgtcgcg ttgttgaccc
    14161 tggatcgcta tctgggcacc catttcttta ccaacgatat gggtggcaac atgatgatgt
    14221 acatcaacct gatttgggcc tggggccacc cggaagttta catcctgatc ctgcctgttt
    14281 tcggtgtgtt ctccgaaatt gcggcaacct tctcgcgtaa acgtctgttt ggttatacct
    14341 cgctggtatg ggcaaccgtc tgtatcaccg tgctgtcgtt catcgtttgg ctgcaccact
    14401 tctttacgat gggtgcgggc gcgaacgtaa acgccttctt tggtatcacc acaatgatta
    14461 tcgccatccc gaccggggtg aagatcttca actggctgtt caccatgtat cagggccgca
    14521 tcgtgttcca ttctgcgatg ctgtggacca tcggttttat cgtcactttc tcggtgggcg
    14581 ggatgactgg cgtgctgctg gcagtaccgg gcgcggactt cgttctgcat aacagcctgt
    14641 tcctgattgc gcacttccat aacgttatca tcggcggcgt ggtcttcggc tgcttcgcag
    14701 ggatgaccta ctggtggcct aaagcgttcg gtttcaaact gaatgaaacc tggggtaaac
    14761 gcgcgttctg gttctggatc atcggcttct tcgttgcctt tatgccgctg tatgcgctgg
    14821 gcttcatggg catgacccgt cgtttgagcc agcagattga cccgcagttc cacaccatgc
    14881 tgatgattgc agctagcggt gcagtactga ttgcgctggg tattctctgc ctcgttattc
    14941 agatgtacgt ttctattcgc gaccgcgacc agaaccgtga cctgactggc gacccgtggg
    15001 gtggccgtac gctggagtgg gcaacctctt ccccgcctcc gttctataac tttgccgttg
    15061 tgccgcacgt tcacgagcgt gatgcattct gggaaatgaa agagaaaggc gaagcgtata
    15121 aaaagcctga ccactatgaa gaaattcata tgccgaaaaa cagcggtgca ggtatcgtca
    15181 ttgcagcttt ctccaccatc ttcggtttcg ccatgatctg gcatatctgg tggctggcga
    15241 ttgttggctt cgcaggcatg atcatcacct ggatcgtgaa aagcttcgac gaggacgtgg
    15301 attactacgt gccggtggca gaaatcgaaa aactggaaaa ccagcatttc gatgagatta
    15361 ctaaggcagg gctgaaaaat ggcaactgat actttgacgc acgcgactgc ccacgcgcac
    15421 gaacacgggc accacgatgc aggcggaacc aaaatcttcg gattttggat ctacctgatg
    15481 agcgactgca ttctgttctc aatcttgttt gctacctatg ccgttctggt gaacggcacc
    15541 gcaggcggcc cgacaggtaa ggacattttc gaactgccgt tcgttctggt tgaaactttc
    15601 ttgctgttgt tcagctccat cacctacggc atggcggcta tcgccatgta caaaaacaac
    15661 aaaagccagg tcatctcctg gctggcgttg acctggttgt ttggtgccgg atttatcggg
    15721 atggaaatct acgaattcca tcacctgatt gttaacggca tgggtccgga tcgcagcggc
    15781 ttcctgtcag cgttctttgc gctggtcggc acgcacggtc tgcacgtcac ttccggtctt
    15841 atctggatgg cggtgctgat ggtacaaatc gctcgtcgcg gcctgaccag cactaaccgt
    15901 acccgcatca tgtgcctgag cctgttctgg cacttcctgg atgtggtttg gatctgtgtg
    15961 ttcactgttg tttatctgat gggggcgatg taatgagtca ttctaccgat cacagcggcg
    16021 cgtcccatgg cagcgtaaaa acctacatga caggctttat cctgtcgatc attctgacgg
    16081 tgattccgtt ctggatggtg atgacaggag ctgcctctcc ggccgtaatt ctgggaacaa
    16141 tcctggcaat ggcagtggta caggttctgg tgcatctggt gtgcttcctg cacatgaata
    16201 ccaaatcaga tgaaggctgg aacatgacgg cgtttgtctt caccgtgcta atcatcgcta
    16261 tcctggttgt aggctccatc tggattatgt ggaacctcaa ctacaacatg atgatgcact
    16321 aagagcggcg gttatgatgt ttaagcaata cctgcaagta acgaaaccag gcatcatctt
    16381 tggcaacctg atctcggtga ttgggggatt cctgctggcc tcaaagggca gcattgatta
    16441 tcccctgttt atctacacgc tggttggggt gtcactggtt gtggcgtcgg gttgtgtgtt
    16501 taacaactac atcgacaggg atatcgacag aaagatggaa aggacgaaga atcgggtgct
    16561 ggttaaaggc ctgatctctc ctgctgtctc gctggtgtac gccaccttgc taggctttgc
    16621 tggctttatg ctgctgtggt ttggcgcgaa tccgctggcc tgctggctgg gggtgatggg
    16681 ctttgtggtt tatgtcggcg tttatagcct gtacatgaaa cgccactcgg tctacggcac
    16741 attgattggt tcgctctccg gcgctgcgcc gccggtgatt ggctactgtg cagtaaccgg
    16801 cgagttcgat agcggcgcag cgatcctgct ggctatcttc agcctgtggc agatgcctca
    16861 ctcctatgcc atcgccattt tccgctttaa ggattatcag gcggcaaaca ttccggtatt
    16921 gccagtggta aaaggcattt cggtggcgaa gaatcacatc acgctgtata tcatcgcctt
    16981 tgccgttgcc acgctgatgc tctctcttgg cggttacgct gggtataaat atctggtggt
    17041 cgccgcggcg gtgagcgtct ggtggttagg tatggccctg cgcggttata aagttgctga
    17101 tgacaggatc tgggcgcgca agctgttcgg cttctctatc atcgccatca cagccctctc
    17161 ggtgatgatg tccgttgatt ttatggtgcc ggactcgcat acgctgctgg ctgctgtttg
    17221 gtaacacaac ctctctatta aaaaggtgct acggcacctt ttttattgtc tgagatcaga
    17281 gatacggcta ataaccgtat gtcttgacag agtgagcaga tgaacataaa ttaatcatac
    17341 ggttgatagc cgtattatgt atgagatcga ctccctacga tttcattcag ttaatcaaag
    17401 aggtcataat gcctggaaaa acagccactc tacccgatgt cgataaaacg ctgaaaaatg
    17461 cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa acttcgtgaa gctgcgcaag
    17521 ccgttggggt agatttaagc gcttttattt tgagcgctgc tatggaacgc gccgaaagtg
    17581 tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag ctgggaactg atgaaccaac
    17641 tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc gttaatgaaa aggaaaaaca
    17701 gcgatggtcg acaagcatga agagattact ctgcccatag tcctctcctg taattatcag
    17761 tctgatatta cttatcctgg gcaaaaacag tttgattgcg gtaactctgt tatcgataaa
    17821 tttgtacgcg catcgctaaa gaaaagtgtg cgtaatagcg actgtgcggc taaagcactt
    17881 attgacagac aaagtggtga actgatcggc atctgtactt ttacggcata ttcgctggaa
    17941 aaacaacgcg tttctggcgt ccttcagggt tcacaacctt cagaaattgg tgttgtcaga
    18001 ttagtcatgt tgggggtagc acggaagtat caaaagcggg gctttggtca ggacctacta
    18061 tgtgattttt ttgaacatgt aaaaataatt caccaggcat taccaattaa aggggtttat
    18121 cttgatgctg accctgccgc cattaatttt tatgctcgtc tcggctttgt tcagctttca
    18181 gcgacaccaa atgcttttgg tgctgtacct atgtttttgg cgattcagca tattctcgcg
    18241 gcttagatta ttatcgccac ccgccagggc aagttttgcc ttagccgggg gctgagcagc
    18301 gttaccactt ttgttcaatt gcccccgctt tcagcaactc cacatccttg ccgttaatgg
    18361 ttgccgttgc ggcaacgatt gtggtcctta cagttgcaca agattttcgc tcaaattccg
    18421 gtttataaag aataagagac gaattactgg taaatgaacc aaataggtaa tcagtaaagc
    18481 taatattcag ccggtttaaa ccgaaaggcg cgaagccaac cgtttggtta atctccttac
    18541 cggatgggcg acgaatgctg aatgtcacgc gaagattggc cacttcgcct aatttattgg
    18601 tggtggcgaa gaggctatcc atgcaggaaa aacctttatc taaaaaatta tctcgcatcc
    18661 cagaacgggt aaccgtaatc acgggtaacg tatcgtcaac cgtttttctg tcaatcatgt
    18721 cttttcgaca agtagccaga aagtcttccg ggccaccata aatgtggaca cctttatcaa
    18781 gatattcctt cgctttctta caatctacct caacatactg accattggcg tacataatgc
    18841 cc
//
LOCUS       DAZPLI010000074        18513 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZPLI010000074 DAZPLI010000000
VERSION     DAZPLI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18513
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.075"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            76..288
                     /gene="cspH"
                     /locus_tag="ACHUU3_002451"
     CDS             76..288
                     /gene="cspH"
                     /locus_tag="ACHUU3_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR2082483.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            735..1040
                     /locus_tag="ACHUU3_002452"
     CDS             735..1040
                     /locus_tag="ACHUU3_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2082484.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTESTTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTH"
     gene            1147..1791
                     /gene="gfcB"
                     /locus_tag="ACHUU3_002453"
     CDS             1147..1791
                     /gene="gfcB"
                     /locus_tag="ACHUU3_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR2082485.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1788..2534
                     /locus_tag="ACHUU3_002454"
     CDS             1788..2534
                     /locus_tag="ACHUU3_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR2082486.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2534..4630
                     /locus_tag="ACHUU3_002455"
     CDS             2534..4630
                     /locus_tag="ACHUU3_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR2082487.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSQLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4676..5815
                     /locus_tag="ACHUU3_002456"
     CDS             4676..5815
                     /locus_tag="ACHUU3_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR2082488.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5803..6249
                     /gene="etp"
                     /locus_tag="ACHUU3_002457"
                     /gene_synonym="yccY"
     CDS             5803..6249
                     /gene="etp"
                     /locus_tag="ACHUU3_002457"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR2082489.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6269..8449
                     /gene="etk"
                     /locus_tag="ACHUU3_002458"
     CDS             6269..8449
                     /gene="etk"
                     /locus_tag="ACHUU3_002458"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFR2082490.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8564..9862)
                     /gene="appA"
                     /locus_tag="ACHUU3_002459"
     CDS             complement(8564..9862)
                     /gene="appA"
                     /locus_tag="ACHUU3_002459"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR2082491.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="ACHUU3_002460"
     CDS             complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="ACHUU3_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR2082492.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10047..11183)
                     /gene="appB"
                     /locus_tag="ACHUU3_002461"
     CDS             complement(10047..11183)
                     /gene="appB"
                     /locus_tag="ACHUU3_002461"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR2082493.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11195..12739)
                     /gene="appC"
                     /locus_tag="ACHUU3_002462"
     CDS             complement(11195..12739)
                     /gene="appC"
                     /locus_tag="ACHUU3_002462"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR2082494.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12873..13730)
                     /locus_tag="ACHUU3_002463"
     CDS             complement(12873..13730)
                     /locus_tag="ACHUU3_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR2082495.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="ACHUU3_002464"
     CDS             complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="ACHUU3_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR2082496.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(14122..14709)
                     /gene="hyaD"
                     /locus_tag="ACHUU3_002465"
     CDS             complement(14122..14709)
                     /gene="hyaD"
                     /locus_tag="ACHUU3_002465"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR2082497.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGRLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="ACHUU3_002466"
                     /pseudo
     CDS             complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="ACHUU3_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="ACHUU3_002467"
     CDS             complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="ACHUU3_002467"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR2082498.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="ACHUU3_002468"
     CDS             complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="ACHUU3_002468"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR2082499.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4548 a         4914 c         4779 g         4272 t
ORIGIN      
        1 gcggtcttaa atatcgctgg ctttatttta ttaaccccgt atagtgcagc gcagtatttt
       61 cagtaaggaa tttgtttgtc ccgtaaaatg acaggaattg tcaaaacctt tgatcgcaaa
      121 agcggcaaag gattcattat cccctccgac ggtcgtaaag aagttcaggt ccatatttcc
      181 gcattcactc cccgcgacgc agaagtgctt ataccaggat tacgtgtgga attttgccgc
      241 gtaaatggcc tgcgaggacc aacagcggca aatgtttatc tctcttaata tgacgctgct
      301 tgtttaaagc tgactggttt tattggaata gtgcaatagt caacatcata cattataaca
      361 tcagttactt ttccacaaag aaactaatcc atttaagcga atatctctgc acctaatagt
      421 tataaatgcc atttaacagc cagttatagt accacttgaa actatattac caacaataat
      481 taatagcttt cgtaacaatt tccttatctt taatttgtat tttgctaaga tttatcttat
      541 gctcacaagt cgtatttcca gaggggatcg ttaataatct tatatttccc tctgcgcacc
      601 tttcctaccc gcagtatcag tatatagtgc aagcggtatg aggaaaggga gatttcacaa
      661 ggatgttata ctcaaagtag acaatcctga tctcaagcgt acgtattgtc catgcgatga
      721 ataaaggaaa agttatgaaa cacaagcttt ctgcaatcct tatggccttc atgttgacga
      781 cgccagctgc gtttgctgcc tctgaagcaa caaacgggac cgagtcaacg acgggtacta
      841 ctggcaccac aacgaccact accggcgcaa ccacgactgc tactaccact ggtggcgtgg
      901 ctgctggcgc tgtcggcacg gcgacagttg tcggtgtggc aacggctgtt ggtgtggcga
      961 cgttggcagt tgttgcagca aatgacagtg gcgatggcgg ttctcataac acctcaacca
     1021 ccacgtccac tacacactaa tactgcttta acgcgtcgaa attaaagtta aatgataccc
     1081 ggttgcgata ttcgtaccgg gctttagaga tttgcatttt tttaattaac ctttcaagga
     1141 ttaaaggtgc gccctcttat tttatcgatt ttcgcactat ttcttgcggg atgtacgcac
     1201 agccagcaaa gtatggtcga tacatttcgc gccagccttt tcgataatca ggatatcacc
     1261 gtagcggatc agcagatcca ggcgttgcct tattccacga tgtatttacg ccttaatgaa
     1321 gggcaacgaa tctttgtggt actgggatat atagaacaag aacaaagcaa atggttatcc
     1381 caggataacg ccatgctggt tacccacaat ggacgtcttt taaaaaccgt caaacttaat
     1441 aataatctgc tggaagtgac aaattccggg caggaccctc tgcgtaacgc gctggcaata
     1501 aaagatggca gccgctggac gcgcgatatt ctctggagtg aagacaacca ttttcgctct
     1561 gcgaccctga gttctacttt ttcctttgct ggattagaga cgctgaatat tgcgggtcgc
     1621 aatgtgctgt gtaatgtctg gcaggaagag gtgacttcca cgcggccaga aaaacagtgg
     1681 caaaacacat tctgggtcga ttcggctact ggccaggttc gtcaaagtcg gcaaatgtta
     1741 ggcgcagggg ttattcccgt agaaatgacg tttcttaaac ccgcaccatg aataaattac
     1801 agtcgtattt cattgccagc gtactttacg taatgacacc ccatgccttt gcgcaaggaa
     1861 cggtgactat ttatctgcct ggcgaacaac agacactttc cgttggcccc atggaaaatg
     1921 ttgtccagtt ggtgacacaa ccgcaactgc gcgatcgcct ctggtggcct ggtgccttgc
     1981 tgaccgactc tgcggcgaaa gctaaagcac ttaaagacta ccaacatgtc atggcacagc
     2041 ttgcttcctg ggaagcagaa gctgacgacg atgtggcggc aacgattaaa tccgtgcgcc
     2101 agcagttact caatctcaat atcactggtc gcttaccggt caaactggac cctgattttg
     2161 tacgagtgga tgagaacagc aatcccccgt tggtcgggga ctatacgctc tataccgtac
     2221 agcgtccggt gactattact ctgctgggtg cggtttctgg ggccgggcaa ttgccctggc
     2281 aggcaggccg tagcgttacc gattatctgc aagatcaccc ccgccttgca ggcgcggaca
     2341 agaataacgt tatggtgatt acgcctgagg gtgagactgt cgtcgcccct gttgccctgt
     2401 ggaataaaag acatgtggaa ccaccgcctg gcagccagct ttggttgggc ttttctgcgc
     2461 atgtactgcc cgaaaaatat gctgatttaa atgaccagat tgtgtctgtt ttaacgcaac
     2521 gtgttcctga ctgatgaaga agaattctta tcttttaagc tgcctggcca ttgccgtctc
     2581 cagtgcctgt catgctgaag tattaaccta cccggatccg ctgggttcgt cgcaatcaga
     2641 ctttggcggc acaggattgt tgcagatgcc aaatgcgcgc atcgcaccgg aaggtgaatt
     2701 cagcgtcaac taccgggata acgatcaata ccggttctac tccacctccg tggcgctgtt
     2761 cccatggctg gaaggcacca ttcgttatac ggatgtgcgc acacgcaaat atagccagtg
     2821 ggaagatttc agcggcgatc agtcatacaa agacaaatca ttcgatttta aacttcgcct
     2881 gtgggaagaa ggttactggc taccgcaagt ggcgtttggt aaacgtgata ttgctggtac
     2941 gggtctgttt gacggtgagt atctggtggc cagcaagcaa gcggggccat ttgatttcac
     3001 cctcgggatg gcatggggct acgccggtaa tgcgggcaat attaccaacc cgttttgccg
     3061 ggtgagcgat aaatattgtc atcgcgcaga gtctcacgat gcgggcgata tcagctttag
     3121 cgatatcttt cgtggcccgg cttccatctt tggcggcatt gagtatcaaa cgccgtggaa
     3181 tcccctgcgt ctgaaactcg aatacgatgg caacaattac cagaatgatt tcgctggcaa
     3241 actgcctcag gcaagccatt tcaacgtcgg cgcagtttat cgcgctgcca gctgggcaga
     3301 tctcaacctg agttatgaac gcggtaacac gttgatgttt ggcttcacgt tacggaccaa
     3361 tttcaacgat ctgcgccctg ccctgcgcga tacgccaaaa ccggcatatc aacctgcgcc
     3421 tgaatctgaa ggattgcagt acaccacagt agcgaaccaa cttaccgcgc tgaagtacaa
     3481 cgcaggtttt gaagcaccgg aaattcagct gcgcgataag acgctgtata tgtctggtca
     3541 acaatacaaa taccgtgatt ctcgcgaagc ggtcgatcgt gccaaccgga ttctggtgaa
     3601 taacctgccg caaggcgttg agaagattag cgtgacgcaa aagcgcgagc atatggcgat
     3661 ggtgactacc gaaaccgacg tagccagcct gcgcaaacag ctggctggta cagcgcctgg
     3721 tcaatcagag caacttcaac aacaacgtgt tgaagcagaa gatctttctg cctttggtcg
     3781 gggctaccgt attcgtgaag atcgctttag ctactctttc aacccaacac tttcacagtc
     3841 gctgggcggc ccggaagatt tctatatgtt ccagctgggg ctgatgtcca gtgcccgcta
     3901 ctggtttacc gaccacctgc tgcttgatgg cggtattttc accaatattt acaacaacta
     3961 cgacaagttt aagtcttcgc agttgcccgc ggactctacc ctgccccgcg tgcgcacgca
     4021 tatccgtgat tacgttcgca atgacgttta tctcaacaac ttgcaggcga actactttgc
     4081 cgacttaggc aatggtttct atggccaggt gtatggcggt tatctggaaa cgatgtacgc
     4141 cggtgtcggt tccgagctgc tttatcgccc gctagatgcc agctgggcgc tgggtgtgga
     4201 cgttaactac gtgaagcaac gtgactggga caacatgatg cgcttcaccg attattccac
     4261 gccaactggt ttcgtgacgg cttactggaa cccgccgacg ctcaatggcg tactgatgaa
     4321 acttagcgtt gggcaatatc tggcaaaaga taaaggggca acgatcgacg tcgccaaacg
     4381 ctttgacagc ggcgtggcgg taggggtatg ggcggcaatc agtaacgtat ctaaagatga
     4441 ctacggcgaa ggcggcttta gtaaaggttt ttatatctcg attccgttcg acttgatgac
     4501 cattggacct aaccgcaacc gcgcggtggt ttcgtggaca ccattgacgc gtgatggtgg
     4561 acaaatgctg tcacgcaaat accagctcta tccaatgacg gcagagcgag aagtaccggt
     4621 tggacaataa cctgacgtaa ttcataacat tacattgact gataaatata aaccaatgaa
     4681 aaagaacatt tttaagtttt cggtactgac attggctgtt ctgtctttga cagcatgtac
     4741 ccttgttcct ggtcagaatt tatcgaccag taacaaggat gtcattgaac tacctgacaa
     4801 tcagtatgac ctggacaaaa tggtcaatat ctatcccgtc acgccaggat tgattgatca
     4861 gttgcgcgcc aaaccgatta tgtcgcaggc taacccggag ctggagcagc aaatcgcaaa
     4921 ctatgaatat cgtatcggga ttggtgatgt gttgatggtt acggtctggg atcacccgga
     4981 actaaccacc cccgcagggc aataccgtag cgccagtgat accggcaact gggttaacgc
     5041 cgatggtgcg attttctacc cctatattgg tcgcctgaaa gtggccggga aaacacttac
     5101 ccaggtacgc aacgagatta cagcgcgtct cgattcagtc attgaaagcc cgcaagtcga
     5161 tgtcagcgtc gcggcgttcc gatcgcaaaa agcgtacgtc accggcgaag tcagcaaatc
     5221 gggtcagcag ccgattacca atattcctct gaccatcatg gatgcgatta acgccgcggg
     5281 cggtctgacc gcagatgcag actggcgcaa cgtggtgctc acccagaatg gcgtaaagac
     5341 caaagtgaac ctctatgcgc tgatgcagcg tggcgacttg cgccagaaca aactgctgca
     5401 cccgggcgat attttattta ttccgcgcaa cgatgatctg aaagtcttcg tgatgggtga
     5461 agtgggtaaa cagagcacgc tgaaaatgga ccgcagcggt atgaccctgg cggaagcgct
     5521 gggtaacgct gagggtatga atcaggatgt cgctgatgcg acggggattt ttgttattcg
     5581 tgcaacgcaa aacaagcaga atggcaaaat cgccaatatt tatcagctga atgcgaagga
     5641 cgcatcggca atgatactcg gaacggaatt ccagcttgaa ccttacgaca ttgtttatgt
     5701 cactaccgcg ccgctggctc gttggaaccg tgtgatttct ctgctggtac cgacgatttc
     5761 cggggtgcat gacctgacag agacttcacg gtggattcaa acatggccca actaaaattt
     5821 aactcaatcc tggtggtttg taccggcaac atttgccgtt cgcccattgg cgagcgcttg
     5881 ctgcgtaaac gtctgccggg cgtaaaagtc aaatcggcag gtgttcatgg tctgataaaa
     5941 caccctgcag atgcgacagc ggcagatgtt gccgctaatc atggcgtatc tcttgaagga
     6001 catgccggac gtaagctcac tgctgagatg gcacgaaatt acgatctgat tctggcaatg
     6061 gagtcggaac atattgctca ggttaccgca attgctcccg aggtccgcgg gaagacaatg
     6121 ctgtttgggc aatggctgga acagaaagag atcccggatc cctatcgtaa aagtcaggac
     6181 gcatttgaac atgtctacgg tatgttggag cgcgccagtc aggaatgggc gaagcgcctc
     6241 agccgataat ttgagtttat aaaatacgat gacaactaaa aatatgaata cgccaccagg
     6301 cagcactcag gaaaatgaga tcgatctgct tcgtctggtc ggcgagttat gggatcaccg
     6361 taagtttatt atcagcgtga ccgcgttatt cacgctgatc gctgtcgctt actcgctgtt
     6421 aagcacacca atttatcagg cagatactct ggtccaggtt gagcaaaaac agggcaacgc
     6481 cattctcagc ggcctgagcg atatgatccc taactcatcg cccgagtctg caccggagat
     6541 ccaactgctg caatcgcgca tgattctcgg taaaaccatt gctgaactga atctgcgcga
     6601 catagttgag cagaagtatt ttccgattgt gggtcgcggc tgggcgagat taaccaaaga
     6661 aaaaccaggt gagctggcga tcagctggat gcatattcca caactgaatg gtcaggatca
     6721 gcaactgaca ctcacggttg gggaaaacgg ccactataca ctggaaggtg aagagttcac
     6781 cgtcaatggt atggtcggcc agcgtctgga aaaagatggc gttgcgctga ctatcgcgga
     6841 cattaaggcc aaaccaggaa cacagtttgt cctgagccag cgtaccgaac tggaagcgat
     6901 taacgcattg caggaaacct ttaccgttag cgaacgcagt aaagaaagcg ggatgctgga
     6961 acttaccatg actggtgatg atccccagtt gattactcgt attctgaaca gcatcgctaa
     7021 caactatttg caacagaata tcgctcgcca ggcggcgcag gattcacaaa gccttgaatt
     7081 cttacagcgc cagttacctg aagtgcgcag cgagctggac caggcggaag aaaaactcaa
     7141 cgtttatcgc cagcagcgcg attcggttga ccttaacctg gaagccaaag ccgttcttga
     7201 gcagattgtg aacgttgata atcaactcaa tgagctgacc ttccgcgagg cagagatctc
     7261 ccagctgtat aagaaagatc acccaactta tcgtgcgctg ctggaaaaac gccagacgct
     7321 ggagcaagaa cgcaaacgcc tgaataagcg ggtatcggca atgccttcca cccaacagga
     7381 agtgttgcgt ttaagtcgtg acgtagaagc gggccgtgcg gtatatctgc aattacttaa
     7441 ccgccagcag gagttgagta tttcgaaatc cagtgccatt ggtaacgtgc ggattatcga
     7501 cccggcagtc actcagccgc agccagtgaa accgaaaaaa gcgttgaatg tggtgcttgg
     7561 ttttattctt ggcctgttta tttctgtggg tgccgtgctg gcgcgtgcga tgttgcgtcg
     7621 tggtgtagaa gccccggaac aactggaaga gcacggcatc agcgtttatg ccactatccc
     7681 aatgtccgag tggctggata aacgcacccg tctgcgtaag aaaaatttat tttctaatca
     7741 gcagcgccat cgtactaaaa atatcccctt cctggcggtg gataacccgg cggattctgc
     7801 tgtggaagcc gtacgtgcgc tacgaaccag tctgcatttc gctatgatgg agacggagaa
     7861 taacattctg atgatcaccg gtgcgacgcc agacagtggt aaaacgtttg tcagttcaac
     7921 tctggcagcg gtgatcgccc agtccgatca aaaagtgtta tttattgatg ccgacttacg
     7981 ccgtggttat tcgcataacc tgtttaccgt gagtaatgaa catggcttgt cggaatatct
     8041 ggcaggtaaa gatgagctca acaaagtgat ccagcatttt ggcaaaggag gctttgatgt
     8101 gattactcgc ggtcaggtgc cacctaaccc gtctgaactg ctgatgcgcg atcggatgcg
     8161 tcaattactg gaatgggcga acgaccatta cgatctggtg attgtcgata cgccgccgat
     8221 gctggcggtg agtgatgccg cggtcgtggg gcgttctgtt ggcaccagcc tgctggttgc
     8281 gcgttttggc ttgaacaccg ccaaagaggt gagtttgtca atgcagcgtc tggaacaggc
     8341 aggcgtcaat attaaaggcg ctatcctcaa tggtgtgatt aaacgcgcca gcaccgctta
     8401 cagttacggc tataactatt acggttatag ttactccgag aaagagtaaa actgaaagtg
     8461 gccggatgcg gcgtgaacgc cttttccggt ctacgcatta gacagttctt cgtttgtcat
     8521 cagcctcaga gcattcaggt aactgaatgc tcttttttat gcattacaaa ctgcacgccg
     8581 gtatgcgtgc ttcattcacg atttgcgtaa aacctgccaa cgaacacatg ccctgcgcat
     8641 ttcgctcttc acatcctgcc agggtcagtt tcacctctcc gggcggcgta tttaatgaca
     8701 gcggcgtttt atcacgcatc tgctgtaaag tctggaagac cagcgaaacc tgaatccact
     8761 ggctgttatc gcttagccga cgccagcgtt caaacaccag ttcaccacct ggcggcgtgt
     8821 tatccggctg accgggaagc gtccagttga gctccagtgc gccgccgaga tttgccagat
     8881 tagtatcgtg tccggcgata aacagcactg aagtgggtaa tgtcacacca tacgcctgtt
     8941 tttgcggtgg atggggcgtc aacgctgtct tgatcaaatc taataacggg gtggcgcggc
     9001 tgcgggcaac ctctggcgtg cgttgtagca aataaaattg cgcgttatgc aaacttagca
     9061 aggtgttcca ctggtgtgaa tcggtgatcc ttccccaccc cggctccggc attccctgtg
     9121 cttgttgcag gagaaatatc tccgtcagca ttgatgcgag gcttaccgca ccggttaatg
     9181 agacattgtc ggcgctcacc ttgagttccg atggtaatgc ctgcgttaat gaacagcttt
     9241 cgtcctgttt ctcacgttta aggcacaagt ttgattgcgg aaaattaagc acccgttcca
     9301 gttcgcgaaa cgccgtttgc cgatgcccgg taaagtcagc aattgaccct cctgccctgc
     9361 tgaggatcgc gtcagtcacg ttcgcgttat ccagttggca aacgccagtt tttagaggat
     9421 taaataacgg atcgggactg gacgtatcag cctgggtatg tacggttatt gcacagtcag
     9481 gtgccagccc ggcggcgaag gcttcgcctg ttttacgggt acgctcgtcg acatcagcaa
     9541 taatcgcgac ctgaccagac tgcgggcagc cctttttcgc cagcaatccg tcggctacca
     9601 gacgctggcg ttggtaatgt ccgagatagg cgattagctc accaccgcgc ggtgtcagcc
     9661 aacccagttt taccggccag gttggccatg cgtctggggt gacatcctgc atcagttgcg
     9721 tggccttggt tggagcacgc acaccatgac gactgacaat caccacactt tccagcttca
     9781 gctccggctc actctgagcg aatgcagatt gcggggttaa cggaatcaga agagataaaa
     9841 atgggattaa gatcgctttc atcgatatgt ttccgctttt catatctgac attattgatt
     9901 gcctgatgcg atgctaatgc gccgcatcag gcctacgttc gttaactttt cagacgcggg
     9961 tccagccata ccaacacaag ggtggagagc gaacacatca acaaaatgcc gacgaaccaa
    10021 agtaaatacc acattgttcc tgctccttag tacaactcgt tttcgttacg gcggagagtt
    10081 tctgttgtca tgcgtcccca cattttgtag tagctccaga gagtgtagag caacacaatg
    10141 ggcaaaaata tcagcacgat taccaacata atgctcagcg tcagctgact ggaagtgctg
    10201 tcccacaacg tcaggctgga gattggactc acgcttgacg gcatgacaaa ggggaacagc
    10261 gtgatgcctg ccgtgaaaat cacgccaaat tgcatcaatg acgccattaa aaatccccaa
    10321 cccgggcgac cacgataaat cgccatcacc gtcagcaatg ggcagaagaa ccccagcagc
    10381 gggaagatcc acaaaacggg cgattcgacg aaattattca tccaggcacc aggtagcact
    10441 gccaccagtt tcattaacgg attggaagga ccgttagcat cctgggcgag cagtacaaag
    10501 ccatcaatac cgacccacag ccagtaaccc gccagcaaaa agcacagcat caccaacagt
    10561 gcagcgcgtt tggtggccag ctgtgaacgc agatgaatca caccaacagt tttcagttgt
    10621 aaccagacgc caccttgcaa aatcaccatc ccaaggctga gcaatccgca caataaaggg
    10681 aatggcgtca gcagttgcca gaagctaccg agatactcca cgcgtaattg cggtgtgaag
    10741 gcaaacggca cgccgagcaa caagttgccg aaggcgatac cgaagactac cggcggcacc
    10801 agactgccga tgaccagacc ggcgtcccac attttacgcc agcgtgcatc ggcgattttt
    10861 ccgcgataat caaaggccag cgggcggaag aacagtgagc acagcaccag gatcatcgcc
    10921 acataaaagc cggaaaacgc cgctgcatac actctgggcc aggcggcaaa taatgcccca
    10981 ccagcgagga tcaaccagac ctggttgcct tcccagtgtg caccaacgct gtttatcact
    11041 atccggcgtt catcatcatt acgcgccacc agcggcagca gacagccgat ccccatgtca
    11101 aatccgtcgg agatcataaa gaccaccagg atcacgccaa tcagcagcca ccagatgaag
    11161 cgcaatgttt cataatcaaa catgattttc ccctttaccc ctgttgctgc gtcggttgtt
    11221 cactctgcat cgcgctcggc cccagacggg catatttctg catcaggtag acttcggcga
    11281 ttaagaacag ggtgtaaagc cctacgatca tgatcagtga gaaagccagt tgtcctgtgg
    11341 ttaaagcgga gtgcgcggag tatgtcggta agatgtcctg tatcgcccac ggctgacgac
    11401 caaactcggt cataaaccac ccggcttcaa tcgcaatcca cggcaacggc aaactccaga
    11461 gcgccatttt cagcacccag cgatgctggt cgattttgcc acgcagcgtc tggacaagcg
    11521 caatcagcat caccagtagc ggcagggaac cacagcccac catgatgcgg aaactccaga
    11581 ataccggcgc aacctgaggt atcgcgccac gcatcgccgc ctggtactgt gcggctgtga
    11641 catgattcat atccggcgca tagcgggaga gcaacatgcc gtagcccagg tcaccttcca
    11701 gttcccggaa tgcctgcaac acatgcggct cacgattgcc ttgcgatatt tcctgcatta
    11761 acagccaggc catacgtccg cgttgcaagc gtgggtaggt ttcagccatc aaatttttca
    11821 gacccggcac ggttttatct aatgagtgag tggcgaggat ccctaacagc gcgggaattt
    11881 tgatggcaaa ggcgttacgc tcttgatcct gttccggcca ggcaaccaca tggaacggtg
    11941 caggagcagg ttccgtttgc cactcccctt ccatcgccgc cagttttacc ggttgtactt
    12001 gcgcgacttc atacgcagaa ctgtctccga gttgtagcgt gccgataatc gccagagtac
    12061 cgaagacgga accgatggca aacgagcgta atgcaacatc gcgctcccgt ccgcgcagta
    12121 aataccaggc gctgatcgcc ataataaaca tggccccggt cacgtagccc gccattacgg
    12181 tgtgcacaaa tttcacctgg ctgaccgggt taaagaccag ctcgctgaag ctggtcatct
    12241 ccatacgtag ggtgtcgata tcaaaatgcg caccggtcgg gtattgcatc caaccgttgg
    12301 cattcaatat ccacaatgca gagagatttg aaccgaacgc caccagccac gtcaccagca
    12361 ggtgctggta tttattcagc cgctgccagc cgaagaagaa aagcccgaca aaggtggatt
    12421 cgaggaagaa ggccattaat gcttccatcg ccagcggtgc gccaaaaata tcgcccacat
    12481 agttggaata gaatgaccag ttagtaccaa actgaaactc catggtcagg ccggtagcca
    12541 cgccaagagc aaagttgata ccgaagagct taccccagaa gcgcgtcata tcgcggtaga
    12601 ttgttttgcc ggtgaccacg taaatggttt ccatgatagc cagcaaaaaa atcagcccca
    12661 gggtaagggg tacaaataaa aagtgataca gcgcggtcag agcaaactgc cagcgcgata
    12721 aatcaatgac atcccacatg cgcactcctg taggcgaaga gagaaaaata acccgataat
    12781 gttagatctc cctctttatc ctgaaactct acgaccagag gtattcgccg attgcgatat
    12841 tcataagact gaaagcgata cttaccgtct ttttacgtcg gtgcagcttc cgccagccac
    12901 tgacatacct cgctaagccg ctgcgcagag tcgaccaaat cttctggcgc tgccagcacc
    12961 acttccggta ttgggcagat ttcataactt tccagtaaag gaccttttaa ggtgtccgta
    13021 cagcgtaaat gccagacatg gcgtaacccc gtggcgttga tatagctctc gccatagcca
    13081 atggtacgaa tctgaatatt tcccggccca cagagacgtg agagaaaaag ccgatcagcc
    13141 tcgctgatgg gtaattgcgt cagattaatg ctgtgcggct gcgcgtcggg gttacgtaca
    13201 tgcgccagta actcatgcgc caacggtagg ccattcatca ggccatcaat gggcggcggt
    13261 aacagcgaat ctgtcggcaa gagattttgc gtggccgcct gccacaacgc cagcggcgcg
    13321 cagccggcct ccagtttgtc ctccagcaac ttttcgccgc gacgtttgcg cacccgccat
    13381 aatccgcaga agatcgcctc ctgtatttca ctttcgctgt cgtcagcctg ctgaatgcgc
    13441 actgacactt ccccttcccc gagaagcgta ttgataaagt gacgatcgtc ggcgttgaga
    13501 tttaacagat cgactgccag cacctcgccc aacggcggct ggcgttgact tagttgatgt
    13561 tgtaactcgt ttaacagcgc aatcacctgc gggctgtgag cacattgctc cagggccgcc
    13621 atactcggtt catcattcac ctgacagctg atcggcagtg gattcatgct gaaactgtca
    13681 tcgttcggtt gcgttcctgg ccccagcaga tggaaaaaag tttcgctcat gaggcacgct
    13741 cctgttgtgg ttcgacaagc ccacgcatca ggtttatcag ttccgctcac gggtgaatac
    13801 cattcagcac accgcgatag tttccgccgg taaacactaa tgtggcggga aagcgaaaga
    13861 cgccaaagcg atctccgatg gcttcgctct gttcaaggtc agcaatcgcc acctgccatg
    13921 tgtagccggg aaactcgcgc agtaattcgc caatcattac cggattatcg ctgacctctg
    13981 gcgtgcgttt cgggtcactg cttaataaca ccacgccgtc tggcgcttgc gtaagccagt
    14041 cgtcaagacg gaattcacta accggcgtcc agccgcgcgc cagcattcgt tgccacaacg
    14101 catcaaatgg cgtgtcgttg ctcatccctg ctcctccagt gtcatcctgt actggcgcaa
    14161 gcgaacgcct tcgtaatttt ccatcgacag acagtcataa ttgagacagc gcgattcatt
    14221 agccggttgc ggcacaattc cccatgcagc cagctgcgcc agcgccgcct gttccgctgc
    14281 gggcagttgc tcccgtgcca gttcgctcag gcggccgccg tagtcgtcga gcattgcggg
    14341 ttgcagaccg acgagggcaa tatgtgctgg cagatgtccg cggatatctg ccagcgccag
    14401 cacttcggag aaactgttct ggtgcaggct cattttcttc gcgctgagat aagccggaat
    14461 acgttctccg gcataggttc gcagcgttcc aggttccagc ccgtagtcaa tggcatcgag
    14521 aatcaacaga tggctggcgc tttcgacata ccccagcaag ttcagtccct gagtaccgcc
    14581 atcgacaatc tccacatact cgggccagtg gtaatgggca tacaaccgtt ccgccacccg
    14641 cacgccgaaa ccttcatcgg cccacagcag gttgcccagc cccattacca caacgcgttg
    14701 ctcgctcatg aacgctcctt gttacttatt ttgccaaatt tgtggctacg gtagccgttg
    14761 accatggtgg agatcaccgt gtcgtcggac atgatgtctt cacgcagcgc catatagaca
    14821 tgaccgatca caaacgcgcc aatcagccac atccccagcc gatgccagct gtgaatgtcc
    14881 attgagttgc ctcccgtcca gtagaaaaat tccaccacat aacggaacgg cgcaaaaata
    14941 gcgtactggc tgtgttcgct gtacagcgca aagccggtga tgatcataaa gaccgacatc
    15001 aggaaatagc cgaacatcgc cgcctgggcg atggggttat ggcctatatc ggcactcgga
    15061 cgttttgcca gaacagatac cagcggattt cataccacac gccctgccac cagcttttac
    15121 gccataccgg cacgataaac agctcgcggg agtatcgatt gccaacaaaa gcccagtaga
    15181 tccgcatcag caagaccacg gtaaaaacca tcccggcgct gaagtgaatt aacctgatgt
    15241 agcccatata gaacagatac gtcgcctcgc cgctgacgga aggtagcggc ttgccgataa
    15301 agtatccggt gaccatcaac accgccatgc ataacaccgt cagccagtgc cagatgcgga
    15361 ctggcgcttc aaagacatag tggctgacaa cgttgtcgct tttctgttgc atgatgattc
    15421 tccttcgctg ttaacgcacc tgcacggaga tcagctcgct accgtcgtcg cccagcacgt
    15481 gtgttgaaca ggcgaggcac gggtcaaagc tgtgcagagt acgcaggatc tccagcggtt
    15541 gctcggggat cgccattttg gtgttcatca gcgccgcttc ataagcgcca atctgccctt
    15601 tgggatcgcg cgggctggcg ttccaggtgg tcggcaccac gcactggtag agatcaatct
    15661 tgccatcgcg aatggcggcc cagtggccta acgccccgcg cggcgcttcg gtaaagccga
    15721 cgccacggca ctctgtcggc caggttgcag gttcccattt ttccgtggag gcggtggcaa
    15781 gattgccgtt tttcaggtta gtcatcagct tgttgaagaa atattgcaac ttacctgcgg
    15841 cccactgtgc ttcgtgcgcg cggcacaaaa tgcggcccaa cgttgactgg ataccggaaa
    15901 gcggcaggtt cagcgccgac atcatgcgat cgaccgactc aacggtcgca gcatcgcctt
    15961 tgtgataagc gattaacgtg cgtgccagcg gccccacttc catcgcgtta ccgcgccagc
    16021 gcggcgcttt gatccacgag tagcgttcct gttcattcag ctgctgaatg ttggtatcgc
    16081 tgcctttgac atcgccgggg ttgtaccacg ggtcggtaat gccatcgaat ggatgacgcc
    16141 cgacctgatc gttgggataa cgataccagg cgtggtcgac aaactcctgc acctgctgcg
    16201 gatcaaccaa atccactggc agcacattgt tgaagtcgcc gttaatcacc gcgccgccag
    16261 gcatcagcag acttttctca ccaaagtcgt tggcaatatc cgggaacgca ccgtagctga
    16321 ggacacattt atcagaaaga cccgtgccga tttcgctcca cggtttgttg aactgaccga
    16381 tggctaaggc gtcggggatc atcacgttgt taatgaagtc cgccgtgcgg gtgataatcg
    16441 actgcaccag gttcaggcgt tccatattga ccgccccgac cgcgccgctt tcgtcaatgt
    16501 taatggcgca aggcatcccg ccgacaatcc agtttggatg cgggttttta ccgccaaaga
    16561 ccgcgtggat tttgacaatt tcacgctgga aatcgagagc ttcgagatag tgggcaaagc
    16621 ccatcaggtt agcttctggc ggcagtttgt actgcgggtg cccccagtag ccattgcgga
    16681 agatccccaa ctgcccgcct tcaacaaatt ttttcaggcg gttttgtacg tcgaagaaat
    16741 agccagggga tgatttcggc caagaggaga gactttgcgc cagttcggag gttttccgcg
    16801 ggtcggcttt cagcgcatct aacacatcga tccagtccat cccggcaagc tgatagaagt
    16861 gcaccagatg atcgtggcac cagagcgttg ccagcataat gttgcggatg atattggcgt
    16921 tgtccggcac tttaataccg atagcatctt cgatggcgta aaccgaagcc agggcgtgta
    16981 cgccagtaca gacgccgcag atacgttcaa cgaacgccca cgcatcgcgc gggtcgcgcc
    17041 cttgtaggat gatctccagc ccgcgaaaca tggtgccgca ggagacggca ttggtgatca
    17101 cattctgatc gttaatattc acttcgcagc gcatgtggcc ttcgatgcgc gtaatcgggt
    17161 cgaccaccag gcggcgtccg gcattattga tggtgtatcc ctgagtttcg tactgagtgc
    17221 tcatgcctgt ttatcctcat tgcctggctg atgttcggtt tctgtaggtt gctggttatg
    17281 acgtctgcgc tggtcaacgg cgctggcgac tgcgtgcaca ccaacagccg ctgccaccac
    17341 accaagcgcg gttaaaccga cggtatcggc ggtggaatga gtacccattt gcggaatatc
    17401 gaccacgcgg ctgtagaacg aaccgcaatc ccagaaacca ttttccgcac agcccaggca
    17461 gccgtgacca gactggattg ggaaagaaac gccatcattc cagcgtgtgg aggaacaggc
    17521 gttataggtg gtaggccctt tgcagcccat tttgtacagg cagtaacctt tgcgggcagc
    17581 gtcatcatcc caactctgga cgaactctcc ggcgtcgaag tgggcgcggc gatagcattt
    17641 atcgtggatt cgctgaccat agaacatcag cggacggccc attctgtcga catctggcaa
    17701 gcgatcaaag gtcaccatgt aagtaatgat ggcgctcatc acatccggga tcggcgggca
    17761 gccaggtact ttgataatgg gtttgtcggt gatgactttg tcgataggcg ttgcctgcgt
    17821 cggattgggc cacgcggcct gcacgcagcc ccaggacgcg caggttcccc aggcgataat
    17881 cgcgctggct ccggcagcgg cacgtttgag tttctcaata aacggtcgac cgctgctgat
    17941 acagaacatc ccctgctcgc ccagcggcgg attaccttct actgcgagga tatatttgcc
    18001 attgtattgc gtgatgatgt cttcgaagac ttcttccgcc tgggttccgg cggcagccat
    18061 caaagtatcg tcataatcga gggaaatcag ggaaaggatg acgtccttcg ccagtgggtg
    18121 agcggagcgg ataaaagatt cggtacagca ggtgcattcc agaccgtgga tccataccac
    18181 cggaatgcgg ggtttgttct ccagcgccca ggcaatcttt ggtgccattc ccgcgcctaa
    18241 tcccagcgac gtggcagcca gactacaata tttgagaaag ctgcgccggg taacgccctg
    18301 acgccgcatg gcctggtaaa atgtttcctc gttattcata tcgcacgtct cttctccttg
    18361 cgacaccggc aggacaccgg gcgcttatgg tcggcaagcg acgtccctgt agcaaggcaa
    18421 accatcgctt cacgtgagat gctgaaaacg aaagctcatc cttctgcact ggcgcacgtc
    18481 gccagaaagt attgttaata aagcgtagtg aaa
//
LOCUS       DAZPLI010000075        18214 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZPLI010000075 DAZPLI010000000
VERSION     DAZPLI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.076"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            15..1091
                     /locus_tag="ACHUU3_002469"
     CDS             15..1091
                     /locus_tag="ACHUU3_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR2082500.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1484..1825)
                     /locus_tag="ACHUU3_002470"
     CDS             complement(1484..1825)
                     /locus_tag="ACHUU3_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR2082501.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHUU3_002471"
     CDS             complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHUU3_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFR2082502.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHUU3_002472"
     CDS             complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHUU3_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR2082503.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3227..3457
                     /gene="holE"
                     /locus_tag="ACHUU3_002473"
     CDS             3227..3457
                     /gene="holE"
                     /locus_tag="ACHUU3_002473"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR2082504.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3559..4215
                     /gene="yobB"
                     /locus_tag="ACHUU3_002474"
     CDS             3559..4215
                     /gene="yobB"
                     /locus_tag="ACHUU3_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFR2082505.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4239..4901
                     /gene="exoX"
                     /locus_tag="ACHUU3_002475"
     CDS             4239..4901
                     /gene="exoX"
                     /locus_tag="ACHUU3_002475"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR2082506.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHUU3_002476"
     CDS             complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHUU3_002476"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR2082507.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKMTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7167..7826)
                     /locus_tag="ACHUU3_002477"
     CDS             complement(7167..7826)
                     /locus_tag="ACHUU3_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR2082508.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHUU3_002478"
     CDS             complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHUU3_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR2082509.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHUU3_002479"
     CDS             complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHUU3_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFR2082510.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9000..10178
                     /gene="purT"
                     /locus_tag="ACHUU3_002480"
     CDS             9000..10178
                     /gene="purT"
                     /locus_tag="ACHUU3_002480"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR2082511.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHUU3_002481"
                     /gene_synonym="eda"
     CDS             complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHUU3_002481"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR2082512.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHUU3_002482"
     CDS             complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHUU3_002482"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR2082513.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHUU3_002483"
     CDS             complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHUU3_002483"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR2082514.1"
                     /translation="MAVTQTTQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14771..15640
                     /gene="hexR"
                     /locus_tag="ACHUU3_002484"
                     /gene_synonym="yebK"
     CDS             14771..15640
                     /gene="hexR"
                     /locus_tag="ACHUU3_002484"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR2082515.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15768..17210
                     /gene="pyk"
                     /locus_tag="ACHUU3_002485"
     CDS             15768..17210
                     /gene="pyk"
                     /locus_tag="ACHUU3_002485"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR2082516.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17270..18112)
                     /locus_tag="ACHUU3_002486"
     CDS             complement(17270..18112)
                     /locus_tag="ACHUU3_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFR2082517.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4671 a         4574 c         4392 g         4577 t
ORIGIN      
        1 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
       61 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      121 taggtaaaat ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat
      181 acaaaaacta ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      241 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
      301 cttataaaat tcgggcaaat caactggaga caataaaaga aaaattggga gagattttac
      361 tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
      421 aaaacacaat ggcaggatca atgcgttctg tgttgtctga tatgtttcgc gaggccattg
      481 ttgaaggacg tatatctcaa aatccagtca cgccaacaag agcaccgaaa atagtagtta
      541 caagagaacg actgaaacta aagatataca actgcatcag ggaggcagca gatcaacttc
      601 cggcatggtt cccattagct atggatttag cccttgtaac aggacaacgt cgcgaagaca
      661 taacgaatat gcggttcagt gatatttatg atgatcgtct ccacgtcagg caaattaaga
      721 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ctacggcttg
      781 gtacagtagt tgaacgatgc cgcctggtaa gccggggaga ttatctaatc agtgccggga
      841 ttagaaaaaa cagccctgac ggcagcattc acccggatgg cctgacaaaa aaatttgtcg
      901 cagccagaaa attcacaggt atccagttca gtgaaaaccc accaactttt cacgagatca
      961 gaagcctggc tggacgattg tacaaagaaa catgtggaga agaatttgct cagcgtctac
     1021 ttggccacac atcggagaag acaacaaaaa tgtatcttga tgagagagaa aaaacgtact
     1081 tactgctctg attttaacgt aaatggattg ttaaatgtat tttggttgtg atataaccaa
     1141 aaagaccgga atacagaaat tcgagtaaat ttcggggaat ttcgggggag acatttgcaa
     1201 ctaattgatt tcaaatgcaa ttaaaaaaag accgaatacg attcctgtat tcggtccagg
     1261 gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc
     1321 cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa
     1381 taaaaagcgc ggtggtcatc agcttaaatg ttaaaaaccg cccgttctgg tgaaagaact
     1441 gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag
     1501 ctcgttctat aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct
     1561 gaatcaccga aatgggttgg gcattggtct tcccactggc aacttccttt tgtgcgatat
     1621 cgtttaaagg atactgcacg agggtactcg gattaataac atacaaagca ttacccggtc
     1681 ggcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac
     1741 gactgacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg agtaacagaa
     1801 acgccagaat actttttttc atcattttcg taatccatca aaaaggttcg agagtcgcaa
     1861 gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaca
     1921 caaaccaggg tgccgcacga tcttcctcct gcctcatgcg cggtacaaga acataacgat
     1981 tagccagcgc aatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc
     2041 cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc
     2101 ttgccagtac gccgatcacc gcaaaatgcc cgcaccatga aaaacacatc agcgcctgaa
     2161 tagcctggtg tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc
     2221 ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg attagtctga tggattttcg
     2281 ccgttacccc ttcattcagt gtcgcatgcc ccattcccgc cagcagaata aattgcgcgg
     2341 tagtgagcat aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg
     2401 ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag
     2461 cctgccaaat gtttggcgaa aatacatctg tccatcccgt tcccatcaac ccgccctgaa
     2521 cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca
     2581 gaaaacgctt cgccagcaag cgacgaatcg tcaagggggc cagccaggca ccgtacattg
     2641 cgaagccaaa aaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa
     2701 acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca
     2761 ccgaaacaac atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa
     2821 caatcagttg cttttggtcc tgctcatttc gcttcgccgg taatgtttta atattttcgt
     2881 tttttggccc cgtgattttt gcaccactga atcctgtttc cacaccttcc gagaagttta
     2941 aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta
     3001 aatgcgcatg tgcccagacg aaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt
     3061 agcgaaggga gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta
     3121 cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatcctg
     3181 gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct
     3241 ggctaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt
     3301 ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc
     3361 tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa
     3421 tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacaggg ttacactttc
     3481 ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat
     3541 tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt
     3601 aaaacctcac tcaccgaaca agtagctcat catctggagt ttgtgagggc cgccgccagg
     3661 caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcgcga
     3721 cgtgcccttc ctgccccacc cgatctctca ctgttagacc cgctttgtta tgccgcaacg
     3781 acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt
     3841 ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga tctaccatca gagtcatggt
     3901 gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg atgaacaacc ggaaggtatg
     3961 gatatggacc cgacctgttc actgtttacg acagggcaat gtctgggaga acccgacctg
     4021 ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt actcgattgc tgtgctgatg
     4081 gcaaatgccc gtggtaacag tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc
     4141 gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc
     4201 attccattac gctaggcttt ttcggcctgg agtatgccat gttgcgcatt atcgatacag
     4261 aaacctgcgg tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa
     4321 aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaagcga
     4381 tggcgattca tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga
     4441 tcccacacta ttacggcagt gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg
     4501 tactgcctga gatgcccggt gagtggattt gcaccatgaa actggcccgt cgcttgtggc
     4561 caggaatcaa gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc
     4621 cgccgggcct gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta
     4681 tcgatattat gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc
     4741 cgtcgttgat gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg
     4801 ccgaacgcga tccgggctat ctgcgctggt tatttaataa cctggacagc atgagcccgg
     4861 agctgcgttt aacactgaaa cattatctgg aaaatactta atccgcaggc gtagcgggta
     4921 atgttccctg cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt
     4981 taaagcggcc agatttaccg ccatggcctg agtccatgtc ggtacagagc aataaaagat
     5041 ggttatcggt tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca
     5101 cctgagaatc gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt
     5161 tgtcatacgg gctgtagctt ttcatgtact cgtaatattg cggatcctgc gggttacccc
     5221 actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt gttacaacat
     5281 caacaaacgg tacctgggcg ataacgccgt ggaataattc cgggcgttga ttaattgcaa
     5341 cgcccatcaa catgcccccc gcactcccgc ccatcgcata acaaagcgaa ggagagccat
     5401 agcccagttt taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct
     5461 tcagaaattt tccgtcttcg taccattgtt gccccagctc accaccgccg cgaacatgga
     5521 caatggcgta gacaaagcca cgatctaaca aactcaagcg gctaaaactg aaatcggcat
     5581 caatacttgc gccgtaagaa ccatagccat acaccagcaa cgggttgtgt cctttgcgaa
     5641 aatgtttgcg atggtagacc aacgaaaccg gaacttcgac gccatcacgg gcgactatcc
     5701 acaggtgttc actgcggtaa ttcgccgcat caaaaccagg aacttccgtt tgttttaata
     5761 cacgacgctc atcggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag
     5821 aataaccata acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat
     5881 aggctggatc atcaaaggca ataccaatga cttcccgggt cttgcggtta atttggcgca
     5941 aactggttaa cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc
     6001 cttccagcat gatgttttcg catggcggaa ttaactcttc ccactgttgc tcatcacgca
     6061 tacgggtacg gtataagcca aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat
     6121 gctggtagtg atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct
     6181 cggcatcggc catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccaaat
     6241 gaatgactac atagtgcttc gaggtcattt tatgcaggct gacgtaatag gtatcgtctt
     6301 tttcttcgta gatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct
     6361 gataaggcag cagcgtcacc ggatgcttgc gaacatagta gaaagtccag gagtcatttg
     6421 cccagacaaa gctgggttca acgttatcca gcagttccgg gtaccagtta ccagtttcca
     6481 gattacgaaa acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa
     6541 tggtgttatc gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagctgcgc
     6601 gcttattggc atcgagcaat atttcccact catcccactc ttcactgaat gccgattgac
     6661 gctggtagat agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt
     6721 tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc
     6781 gatcctgcaa ggcttgttgt gaggccatca cccgatgacc gtaactattt tcttgttgca
     6841 ggtagtccag gacttctggc tgagaacgcg tatcatcccg cagccagtag taattatcga
     6901 tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt
     6961 tattgttctt tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc
     7021 gaaacagggg agtcattgct tagatgatga caggtaatgg cacggatatc gaatgttatg
     7081 cagacagaga aactcagcct gttcaaatgg ctgtgcggtt ctggatagcc cgaaatagtc
     7141 aacttcaggc tatccagaga gcggaattat tccgccaaag tgcgtttttg ctgttcgaga
     7201 tcgcgttcaa tgccgtcacg aacatcctgg gggattttca gcgcgtcacc cagtgcattc
     7261 aggtaactgc gttccataaa atggtcaata tcaatagccg cgcaactcag gaaatagatt
     7321 tccagcgcct cttcttcatt gcggaccccg gtagccaggc gttgtggatc cagcggttgt
     7381 tcgattgcct gctcaatgag tacacgcccc tgctcttcca cgccggcttc acgcaattgc
     7441 tggtcgattg ccgcacgttc tttggcatca atatgaccat cacttttagc ggcaaagacc
     7501 agcgcaagga tcaaacgttc tgtacgctca tccagcggcg tactttgcgc gccaaactgc
     7561 ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc
     7621 gctaccgctc cgccgccaac cagtaacgcg ttggtgccat atttcgtaag aagtttacgt
     7681 gctgatttat ttgcgaccag cagcccagcc agtccgccta atgcgcctgg cactaacagt
     7741 ttgcccaatc cctgatccgc agacgaggag gtagaagaac tgctttgccc aagcagggat
     7801 tgcagttgat ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta
     7861 gcatagcgga tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg
     7921 ggcggtaggc cgttattcaa agaaattcgc gccaggtaag gtatttactg tatatcaggt
     7981 gataagggta ttttaggtga aagtagtttg tcgttttgag tggcattgtt tgatgctgat
     8041 tgcccgatgc gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg
     8101 acaaggcact cgcgccgcat ccaccaacgg tcaatcgaat tatcagaagc cattaacgcc
     8161 gctgatattc cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac
     8221 ttttaccacg cacggttagc ggtactgacc atttatcatc tttaccctga atgtcctgca
     8281 aactgaccca agccacggga tcggcctgac cgacaatttt ttgatcatct gcccaacgcg
     8341 ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc aattccggca gcatccagac
     8401 cttcacactt tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg
     8461 cgcaggcaga aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca
     8521 tagcacaatg attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg
     8581 ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt
     8641 tgccggtttt cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaaggg
     8701 cttcacgttg ctcgtcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat
     8761 ccgccgtatc aagcattttg tcataagcat cggcttcctt tttgctggta aacgacattt
     8821 tctcttcgcc ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat
     8881 aataactgtg attttataca gtatatttct tttcggttga gaaatcaaca tcagcaataa
     8941 agacacacgc aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta
     9001 tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg
     9061 gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg
     9121 tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata
     9181 tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc
     9241 cggagatcga agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg
     9301 ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg
     9361 cggcagaaga gctgcagctg cccacttcca cttatcgttt tgccgatagc gaaagccttt
     9421 tccgcgaggc ggttgctgac attggctatc cctgcattgt aaaaccggtg atgagctctt
     9481 ccggcaaggg gcagacgttt attcgttctg cagagcaact tgctcaggca tggaagtacg
     9541 ctcagcaagg cggtcgcgcc ggagcgggcc gcgtaattgt tgaaggcgtc gttaagtttg
     9601 acttcgaaat taccttgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg
     9661 taggtcatcg ccaggaagat ggcgactacc gtgaatcctg gcaaccacag caaatgagcc
     9721 cgcttgccct tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt
     9781 atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaggtct
     9841 cccctcgtcc acatgatacc gggatggtga cgttaatttc tcaagatctt tcagagtttg
     9901 ccctgcatgt acgtgccttc ctcggacttc cggttggcgg gatccgtcag tatggtcctg
     9961 cagcttctgc cgttattctg ccacaactga ccagtcagaa tgtcacgttt gataatgtgc
    10021 agaatgccgt aggcgcagat ttgcagattc gtttatttgg taagccggaa attgatggca
    10081 gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg
    10141 cgaagcacgc cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa
    10201 aaatgcccga tcgtagatcg ggcgttttga cttttacagc ttagcgcctt ctacagcttc
    10261 acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag
    10321 ccaggaacca ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg
    10381 agaaatacca cccgtcgggc agaaacggac ctgggagaac ggacccgcga tcgcctgcag
    10441 ggctttcacg ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc
    10501 catacccagc atcagttcgg aaacagtgct gatccccgga attagaggaa tagtcccttc
    10561 ggtagcagct ttcagcagcg gctcggtcag acccgggcta attgcgaact gtgcacccgc
    10621 ttcagtgact tctgccagct gctgtggatt cagcaccgta ccggcaccca caatcgcttc
    10681 aggcacttct ttggcgatag cacggatagc gtcaactgca cactcggtac gcagagtcac
    10741 ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc atcggcactg cgtgttccag
    10801 tttttttacc acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt
    10861 tttccagttt ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt
    10921 gatacaggtt gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc
    10981 acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag
    11041 ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc
    11101 cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc
    11161 tgacggcact ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa
    11221 acaccggtcc aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt
    11281 cgcttttggc ccctgatgac ggacaacaac gacacaatcg cggtccagca aacccgcttc
    11341 aaaggccggc ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg
    11401 gttctcaacc ggcacggcag aggttttcat aaccgcacgg cccaggttac cgcttaacac
    11461 ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag
    11521 tgatttttcc gccccttccc gccagtcctg ttcaccatta ttcagccatg gttcaagggt
    11581 ataacgagac agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt
    11641 gagcagttca cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc
    11701 cggaccgttc gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc
    11761 atcccagtta atctgaatac cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt
    11821 agtggaacca ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat
    11881 catcttaccg atcggcatcc attcattacc attaccggtc atgcgtgtaa cctgacgcgc
    11941 agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg
    12001 caactgcatc cccataaatt ccaccaccat ctggttggtg ttggcagtac cgtagaaagt
    12061 acatgttccc ggcgcatggt aagacgcggc ttctgactcc agtaaggcca tgcggtccac
    12121 tttaccttcg gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc
    12181 catcggtcca gacggcacaa acaccgcagg caaatgacca agcgacaggg ctgccatcgt
    12241 cagacccggg acaatcttgt cgcacacacc gaggaacaga gcaccatcaa acatgttatg
    12301 ggacagcccc accgccgcag acatcgctat cacttcgcgg cttagcagcg acaattccat
    12361 tccatcctgc ccctgggtga caccatcaca catcgccgga acaccgcccg caacctgacc
    12421 aaccgcattc gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg
    12481 gtgcgcggag agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct
    12541 tttcaaagag gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca
    12601 tgccaactgc gaacgatgaa cggtcgacgt tttcgcttgt tctatccggg cgagataagc
    12661 agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt acgcataaca attgtggatt
    12721 cataaaggct cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcattcag
    12781 aggatttatg agtgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt
    12841 gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct
    12901 acaatctgtg caagatcatg ttaccggtaa aataaccata aaggataagc gcagatatta
    12961 ctcaaactca ttccaggaac gaccatcacg ggtaatcatc gccaccgagg caacgggtcc
    13021 ccaggttccg gcctgatacg gtttcggcgc atcattgtcc atcgcccacg cctcagtaat
    13081 ggagtctacc catttccagg cttcttccac ttcatcacga cgtacaaaca gtgcctgaat
    13141 accacgcatg gtttccagca gcagacgttc ataggcatcc gccagatgcg tctgattaaa
    13201 ggtttctgaa tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc
    13261 aggaacttta ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag
    13321 tttattctgc ggcagatcct gccacgactc tttaaacaga ttcagttcag gtgttttgaa
    13381 atagaccacg acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg
    13441 cacaccggcc cagcgccagt tatcaatgtc gacgcggatc gccacgaagg tttctgtatt
    13501 gctgctcttg ttcgcgccct cttcttccag atatcccggc acttttttgc cctgggcgaa
    13561 gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg
    13621 cagggacttc agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagatgg
    13681 cggagacatc gcaatcatgc agagaatttg tagcaggtgg ttctgaatca tgtcacgcat
    13741 ctgaccggct ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt
    13801 aatctcaaca tgatcaatgg tgcgattgtc ccagttattc acaaacaggg agttagcaaa
    13861 acgcagcgcc aacaggttca gcaccgtttc tttaccaaga tagtggtcga tacggtaaac
    13921 ctggcactcc tcgaagtatt cgccaacctg atcattgatt tcctgcgagg tcgccagcga
    13981 cgtccccagc ggtttctcca tgactacgcg tgccggttta gcattcagtt ttgcctcgcc
    14041 aagccctttg caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat
    14101 acgatttttt tgatccagca tcgcgccgag acggttgaat gcagcagtgt cattgacatc
    14161 gagattacaa aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatggtttc
    14221 tttcatgaaa gtttcgagcg cctcgcggac aactttggta tatgccgctt tatcccagtc
    14281 agcacgccct acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg
    14341 atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag
    14401 gtcacaggcc tgggttgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg
    14461 gtacttaagc cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc
    14521 ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg
    14581 gcaaaactga taaaaaaatc atcgttttcc attcggtaaa aatctgacac tgatcatgtt
    14641 atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt aacacggcac acaggcgtaa
    14701 tatctgacaa aactgcattt cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc
    14761 tctttacatc atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc
    14821 agagcgcaaa gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat
    14881 tgctgctatg gcactggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag
    14941 catggacacg cgcggttttc ctgattttaa acttcatctg gcacagagtc tggcgaatgg
    15001 cactccctat gttaatcgca atgtcaatga agatgacagc gttgaatcat acacagggaa
    15061 aatatttgag tccgcaatgg caacgcttga tcatgtccgt cattcactgg ataaatctgc
    15121 catcaaccgc gccgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg
    15181 ctcttcagcc gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt
    15241 ggtgtactcc gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga
    15301 cgtggtggtg ctgatttctc acactggaag aacaaaaaat ctggtcgagc tggcgcagct
    15361 ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct gcgggtaccc cgctcgcccg
    15421 ggaagcaacg ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat
    15481 ggtttctcga cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg
    15541 acgcggtgca aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg
    15601 ttttgataag cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta
    15661 atcttatacg acatccgaat gagattaatt tatcgccatc gcggcgttat ttcattcgga
    15721 tttcatgttc aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc
    15781 ttcgcagaac aaaaatcgtt accacgttag gcccagcaac agatcgcgat aataatcttg
    15841 aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa cttttctcac ggctcgcctg
    15901 aagatcacaa aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg
    15961 tggctattct gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca
    16021 aagttttcct caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag
    16081 gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg
    16141 acatcctgct gctggacgat ggtcgcgtcc agttaaaagt actggaagtt cagggcatga
    16201 aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac
    16261 ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg
    16321 cggcgttgat tggcgtagat tacctggctg tctccttccc acgctgtggc gaagatctga
    16381 actatgcccg tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg
    16441 aacgtgcgga agccgtttgc agccaggatg caatggatga catcatcctc gcctctgacg
    16501 tggtaatggt tgcacgtggc gacctcggtg tggaaattgg cgacccggaa ctggtcggca
    16561 ttcagaaagc gttgatccgt cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc
    16621 agatgatgga gtcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag
    16681 caaacgccgt tctggatggt actgacgctg tgatgctgtc tgcagaaact gccgcagggc
    16741 agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc
    16801 cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcta
    16861 ttgccatgtc agcaatgtac gcagctaacc acctgaaagg cgttacggcg attattacca
    16921 tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctaccaattt
    16981 tcgccatgtc gcgccatgaa cgtacgctga acctgactgc tctctatcgt ggcgttacgc
    17041 cggtgcactt tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc
    17101 tgcgcgataa aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga
    17161 tgagtaccgt gggttctact aataccacgc gtattttaac ggtagagtaa tctcacctgc
    17221 cccggccgct gatggccggg gtatttagca acacataaat taggactact tattcaatcc
    17281 aatcataatc acagccccct gtaaaatgaa tacagggata gcctgaatgg agatctaaat
    17341 agcttgcaag acgtcgcata cccgcttcct ctgcaacact atgtccaata actgccatcg
    17401 gtaagccact atctttggag tattgtgtct gcacccatgt tttaattgaa tcgttaatcg
    17461 taatataata atcagcctga tattccatat attgtattgg atcacaaaaa cagcctgccc
    17521 caatgccaac agttctgact actctggctt tatctccata aaattgaatg ccaggaagat
    17581 tatatatttt caaatttttg gcaaaacgtt tggtaatgtt aatggcactg tcagggtcta
    17641 tagcatatac acagtacatc tcatcagaag caacaatatt tgtaacatcc agttctaact
    17701 gctgagccaa agccttagac attccgaacg taggctctcg atccatgaca tcatggcaac
    17761 gaatgattac caaattattt ttatgcaaaa actccttttt ttgctcaatt atttttaaat
    17821 aactgagttc accgcgagta taatccatta tttttctggc ccatgaaaac tcaagatcgt
    17881 caccctccag atcagcataa gaataaaatg taggctcatg tgtaataatg atattgacac
    17941 cttcaaaaac agcctttctt aaaacatcca ttgaggcaat ccagcaggtc ccaattttat
    18001 taataagtgt cgcctcagca ccaaaaataa ttttatcaac agttggttgc gaaacagcaa
    18061 ccattgaatt taaaaaacaa ttaagatccg cggctgtttt aatatgatgc attcaacatc
    18121 cttcacaggt ttcatcagca ataaaataat ttcaacattt ctgggaaacg ttttgcccat
    18181 gcagactcag agtgggtatc acctccccat aaat
//
LOCUS       DAZPLI010000076        17956 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZPLI010000076 DAZPLI010000000
VERSION     DAZPLI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17956
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.077"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(227..1201)
                     /gene="cysB"
                     /locus_tag="ACHUU3_002487"
     CDS             complement(227..1201)
                     /gene="cysB"
                     /locus_tag="ACHUU3_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR2082518.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1411..4008)
                     /gene="topA"
                     /locus_tag="ACHUU3_002488"
     CDS             complement(1411..4008)
                     /gene="topA"
                     /locus_tag="ACHUU3_002488"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR2082519.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4388..4639
                     /locus_tag="ACHUU3_002489"
     CDS             4388..4639
                     /locus_tag="ACHUU3_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR2082520.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4675..5724)
                     /gene="sohB"
                     /locus_tag="ACHUU3_002490"
     CDS             complement(4675..5724)
                     /gene="sohB"
                     /locus_tag="ACHUU3_002490"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR2082521.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5944..6702
                     /locus_tag="ACHUU3_002491"
     CDS             5944..6702
                     /locus_tag="ACHUU3_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR2082522.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6699..7289
                     /gene="cobO"
                     /locus_tag="ACHUU3_002492"
     CDS             6699..7289
                     /gene="cobO"
                     /locus_tag="ACHUU3_002492"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR2082523.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7329..8201)
                     /gene="rluB"
                     /locus_tag="ACHUU3_002493"
     CDS             complement(7329..8201)
                     /gene="rluB"
                     /locus_tag="ACHUU3_002493"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR2082524.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8414..9528)
                     /locus_tag="ACHUU3_002494"
                     /pseudo
     CDS             complement(8414..9528)
                     /locus_tag="ACHUU3_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9556..10176)
                     /locus_tag="ACHUU3_002495"
     CDS             complement(9556..10176)
                     /locus_tag="ACHUU3_002495"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR2082525.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(10173..11054)
                     /gene="rnm"
                     /locus_tag="ACHUU3_002496"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(10173..11054)
                     /gene="rnm"
                     /locus_tag="ACHUU3_002496"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR2082526.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11192..11236
                     /gene="trpL"
                     /locus_tag="ACHUU3_002497"
     CDS             11192..11236
                     /gene="trpL"
                     /locus_tag="ACHUU3_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR2082527.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11213..11306
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11328..12890
                     /gene="trpE"
                     /locus_tag="ACHUU3_002498"
     CDS             11328..12890
                     /gene="trpE"
                     /locus_tag="ACHUU3_002498"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR2082528.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12890..14485
                     /gene="trpD"
                     /locus_tag="ACHUU3_002499"
     CDS             12890..14485
                     /gene="trpD"
                     /locus_tag="ACHUU3_002499"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR2082529.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14486..15847
                     /gene="trpCF"
                     /locus_tag="ACHUU3_002500"
     CDS             14486..15847
                     /gene="trpCF"
                     /locus_tag="ACHUU3_002500"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR2082530.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15859..17052
                     /gene="trpB"
                     /locus_tag="ACHUU3_002501"
     CDS             15859..17052
                     /gene="trpB"
                     /locus_tag="ACHUU3_002501"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR2082531.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            17052..17858
                     /gene="trpA"
                     /locus_tag="ACHUU3_002502"
     CDS             17052..17858
                     /gene="trpA"
                     /locus_tag="ACHUU3_002502"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR2082532.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4317 a         4749 c         4488 g         4402 t
ORIGIN      
        1 cagcgaattt acgaatcgtc tccagtgaat aattcaatct tttgacctga aatagatatt
       61 tgaaaactgg agtttgcgag gcgggtaatt agacactact ttacaggtaa tttaccctat
      121 aaatatgagg gataaaaata ttgtgcacgg tatagaaata agtaacaaaa gagaatatat
      181 tccggcacct tcgctacata aaaggtgccg aaaataacgc aagaaattat ttttccggca
      241 gttttatatc tttaaacatg acctcaattt cttcattaga gcgcaatgcg acagccgcat
      301 caacgacatc acgcgttaaa tgcggtgcaa aacgctgaat gaaatcatac atataactgc
      361 gcaagaaagt actacggcga aaaccaattt tggttgtact gtggctgaag atatcgtgag
      421 catcaacacg cacaaggtcg ggatcggcga ccggatccac cgccatgctg gcaatgaccc
      481 ctacccccag ccctaaccgg acgtaagttt taatgacgtc agcatccgtt gccgtgaaga
      541 cgatacgcgg cgttaaccct gcgcggttaa aggcagtatc cagttctgaa cgtccggtaa
      601 agccgaaggt atatgtcacc aacggatatt gcgccagttc ttcaatggta atggcttttt
      661 tgcctgccag cgggtgatcc ggagtgacta caatagcccg attccagtgg tagcacggta
      721 acatcactaa atcttcatac agatgcagcg cttctgtggc gatagcgaaa tcagcattgc
      781 ctttagagac ggcatcagca atttgtgtcg gcgagccctg gtgcatatgc aaagaaacgc
      841 ggggataacg ctcaataaag cctttgataa cgtttggtaa cgcgtagcgt gcctgggtat
      901 gcgtggtggc gatatacagt gagcctttat ccggccaggt gtgctctccg gcaaccgatt
      961 ttatggcatc gactttcgac aggacttcgc gagcgatacg aattatttct tgccctgctg
     1021 gcgttacctg cgtcaggtgc ttgccgcttc gggaaaaaat ttgaatgcct agctcgtctt
     1081 ccagcattct gacttgttta ctgatcccgg gttgtgatgt gtaaagtcct tccgctgttg
     1141 atgagacatt caggttatga ttgaccacct caacaatata gcgaagttgt tgtaatttca
     1201 tgttaaacca tccacttaga cgccatcaga acatcgtttt ttcgtcacac ctggcgggtg
     1261 actaatacga tttaataata aaaaaggtgc taactataac cactatatca tttatagcct
     1321 tagtgtatag gtgtgaagaa aaaataatat gcaataaaaa agggccgctt tcgcgaccct
     1381 ttgtttataa aaacctgaca gaattaaagg ttattttttt ccttcaaccc atttgccatc
     1441 aacataaaat gctgaccagc cagtcgcctt tccgtctttt tccgaagaga catattgctg
     1501 tttggtttta cggctaaagc gaaccatggt cttattacct tccggatcct gctgtggcgc
     1561 atcggccaga taacgcagtt tttccggcag acggtcgcgg aagcgataaa gctcttccac
     1621 cagtggcgca cgcgtttcac gcgatttcgg gaaagtgttg gcagccagga aaacacccgc
     1681 agcgccgtca cgcagcacga aataagcatc tgatttttcg cacggcagct caggtaatgg
     1741 caccggatct tctttcggtg gtgccacttc gccgttacgt aaaatcttac gtgtgttttt
     1801 acactcttcg ttggtgcagg ccatgtattt accgaaacgc cccattttca ggtgcatttc
     1861 agagccacat ttttcacact caacgatcgg gccgtcataa cctttaatgc ggaattcgcc
     1921 ctcttcgatc tcgtaaccgt cgcaggttgg gttattacca cagacatgca acttacgttt
     1981 cggatcgatg agatagctgt ccatcgccgt gccgcatttc gggcaacgac gttttgcgcg
     2041 cagcgcgttg gtttcagcgt cttcgccttc cagtacgttc agcacttcgt tttccggcac
     2101 caggttaatg gtggttttgc aacgctcttt cggcggcagc gcataaccag aacagccaag
     2161 gaataccccg gtgctcgctg tgcgaatccc cattttgcga ccacaagtcg ggcagtcaat
     2221 gctggtcaga accatctggt tcgggcgcat accaccctct tccggatctt tttcagcttt
     2281 atctaactgc tgggtgaaat ccgagaagaa gtgatccagt acagctttcc actctgcttc
     2341 gtgatttgcc accaggtcga ggctgttttc catctgtgcg gtgaagtcgt agttcattaa
     2401 ctcgcggaaa ttttcttcaa ggcgatcggt gacgatttcg ccaatttttt ccgcatagaa
     2461 acgacgattt tctactcgca cgtagccacg atcctgaatg gtcgaaatga tcgacgcata
     2521 ggtagacgga cgaccgatac cgcgtttttc cagctcttta accagcgatg cttcactgaa
     2581 acgggctggc ggcttggtaa agtgctgggc tggtgtaagt tcaacgagcg tcagagcatc
     2641 gcctttatta actgctggta agatgcgatc ttcatcgcct ttacgcaacg caggcatcac
     2701 ttttgtccag ccatcaaaac gcaaaatacg accgcgtgct ttcaggcgga aatcgcccgc
     2761 accaatggtc agcgtcgtgg agtcatattt cgctggggtc atctggcagg ctacgaactg
     2821 acgccagatt aactggtaca gtttctgcgc atctgcttcc atatccttca gcgattccgc
     2881 catcacattg acgtcagaag gacgaatcgc ttcgtgcgct tcctgtgagt tttctttgct
     2941 ggcgtactga ttcggacttt ccggcagata tttcttacca aaattatcgc tgatataacc
     3001 acgaaccata tttaccgcgt cctgactcag gttagtggag tcggtacgca tgtaagtgat
     3061 atagcctgct tcatacaaac gctgcgccat catcatggtt tttttcacgc caaatccaag
     3121 acgggtgctg gcagcttgtt gcagcgtaga ggtaataaaa ggagcgccag gtttactggt
     3181 tgtcggtttg tcttcacgtt ccagcacgct gtagcgcgct ttttccagca gacttaccgc
     3241 agcctgagtt tgttctttgt tgaccggacg gaacggtttg tcgttctgat gagtcacctg
     3301 taacgccaac gcttcaccag atggcgtggt cgtgctggca tcgacttccc agaactcttc
     3361 cggcacgaac gctttaattt cacgctcacg ctcgaccacc aggcgaaccg ccaccgactg
     3421 cacacgaccg gcagacagac cacgagcgat ctttttccat agcagcggcg aaaccatata
     3481 ccccaccacg cggtccataa agcgacgcgc ctgctgggca ttaacacgat caatattcag
     3541 ctcacccggt ttgttaaatg cctggcggat cgcgttttta gtaatttcgt taaacaccac
     3601 tcggctatag cgcgcatcat cacccccaat cacttcccgc aggtgccatg caatggcttc
     3661 cccttcgcgg tcaaggtcgg ttgcgagata gatgtggtcg gctttttcag ccagttgttt
     3721 cagttcagag acgaccttct ctttaccagg caacacttca tagtgcgcct cccaattgtg
     3781 ccacgggtca acccccatac ggttgacgag agcgccacgt tcatcctttt taggcttttt
     3841 agccgtcttg gtggaggtag agtcggcact ctttttggca gctgagccac tggtcggcaa
     3901 atcgcggatg tgaccgacgc tggatttcac cacgtagtca ctacccagat acttgttgat
     3961 cgttttggct tttgccgggg actcaacgat gacaagagct ttacccatat tcaccttacc
     4021 taatttgatt cttccaggaa tgcgtcgcac gttgataaac cttccactgg caacgagtta
     4081 ccgatatgga ctcgttctct gcggatatca accccttgtc gtgactctgt tcacgaaaat
     4141 tcaggtgact gagttaacag gtcttggcct acaggcgcta tagcacgacg gaattttggt
     4201 cgaatgtcaa gcaaatctat tgccagattg acgaaagcgt cacactgtac ctgataaaat
     4261 ccgatacgca actttattag catgcaaaat ccaaagttgc cagtaaggcg ctcttgccac
     4321 cagcgtggta tatttgtctg caagccatgc ctggcgtaga ctaatgtcct tgtttaagga
     4381 gtaaataatg aacaaagaga cacaacccat cgaccgcgaa acgctgttga aagaggccaa
     4441 caaaatcatt cgtgagcatg aggatacgct cgcagggatt gaagccaccg gcgtcacgca
     4501 gcgtaatggc gtgctggtat ttatcggcga ctacttttta gatgaacaag gattgccaac
     4561 agccaaaagc accgcggtgt ttaatatgtt caaacacctg gcccatgtgc tttcagaaaa
     4621 atatcatctg gttgattaaa gcaaaacgcg aggtcttagc ctcgcgtttg tcttttacat
     4681 caatggcttt tgaccccgct gccaccagcg tagcaacaat cgatcggcgc tctctgccgc
     4741 gctgccggtg aatcggtcaa tgagtcgttt acgctgcata tagcgtacat taaccacttc
     4801 acggccttcc atcaggctaa gaataacttc atcactggtg ttgatttcat caaccaggcc
     4861 tttctctacc gcctgttgtc cgtaccagtg ttcacccgtt gccacctgtt caatatccag
     4921 agacggacgc atacgcttca caaaatcttt aaataactga tgcgtttcgt tcagctcttc
     4981 gcggaatttc tcccgccctt cttcggtatt ttcacccagc aaggtcagcg tgcgcttata
     5041 ctgcccggcg gtgtgcagtt cgatatcaat atctttgctt ttcaggaagc ggttaaagtt
     5101 gggcatttgc gccaccaccc caatggaacc cacaatagca aacggtgcgg aaacaatttt
     5161 gtccgccaca caggccatca tgtaaccgcc gctggcagcg actttgtcta ccgtaacagt
     5221 taaaggaatg tttttatcac gcagacgctg cagctgcgaa gccgccaacc cgtaaccatg
     5281 caccatgcca ccagggcttt ccagacggag cacaacctga tcctgcggtt tgaatgctgc
     5341 gagtaccgcc gttatctctt cacgtagcga gttcacttca tgggcgtcca tgctgccttt
     5401 aaaatccagc acccagacgc gaggtttact gtcagttgcc acctcgccca gtttggcttt
     5461 cgcttttgct gctttcgctt cttgcttgtg ctttttcttc tgcgctttgt gccactgttt
     5521 ttgctgatgt gagtccatca gcgccgcggc cagttcttct ttcatctcct tatactgttc
     5581 gctgagattg ttgacccgta actcgccacg ctggcgttta ttacgttgag caacattgac
     5641 aataatggcg gcaatcgccg caatcgctag cacaacggta acgattttcg ccaaaaacaa
     5701 accatattca gacaacaatt ccacgagtcc ccaccttggt taaacaacgc tttggctcac
     5761 agtttacaac aggcatgtta agccgtctcg cagagatgtt attgcagcga aagtatgaca
     5821 aagtatccat tttgcggtgt tatcgcccca ggctgattga aatgcccttc tgtttcaggc
     5881 ataaagcccc aaagtcataa agtacactgg cagcgcgaca gacaacgtcg ttgaggagta
     5941 gccatgcatt accagccaaa acaagattta ctcaatgatc gcattatcct ggtgacggga
     6001 gccagcgatg ggattggtcg tgaagccgcg atgacgtatg cacgctatgg tgcgacagtg
     6061 attctgttgg gccgtaatga agaaaaatta cgtcaggtag ccagccacat aaacgaagaa
     6121 actgggcgtc agccacagtg gtttattctc gatttgctga cctgcacgtc cgaaaattgc
     6181 caacaactgg cacagcgcat tgtcgttaat tatccgcgtc tggatggtgt tttgcataat
     6241 gccggattgc tcggcgatgt ttgcccaatg agcgaacaaa atccgcaggt ctggcaggac
     6301 gtcatgcagg tcaacgttaa tgccaccttt atgctcaccc aggcactgct tcctttatta
     6361 ctcaaatcgg acgccggttc actggtcttt acttcatcaa gcgttggacg tcagggacga
     6421 gccaactggg gtgcatatgc agcgtcgaaa tttgccaccg aagggatgat gcaggtactg
     6481 gccgatgaat atcagcagcg cctgcgcgtc aactgcatta acccaggcgg tacgcgcacc
     6541 gcaatgcgcg caagcgcctt cccgaccgaa gatccacaga aacttaaaac acccgctgat
     6601 atcatgccgc tctacctctg gctgatgggc gatgacagcc gccgtaaaac cggcatgacc
     6661 tttgacgccc aaccgggccg taaaccagga atttcccaat gagtgatgaa cgctaccaac
     6721 agcgtcagca gcgagtgaaa gaaaaagtag atgctcgtgt ggcccaggcc caggatgaac
     6781 gcggtattat catcgtcttt accggcaatg gaaaaggcaa aaccaccgcg gcatttggta
     6841 cggcaacacg cgcagttggt cacggaaaaa aagtgggcgt cgtgcagttt attaaaggca
     6901 cctggcctaa tggcgaacgc aatctgctgg agccacatgg cgttgagttt caggtgatgg
     6961 caacgggctt tacctgggat acacaaaacc gcgagtctga taccgccgcc tgccgcgaag
     7021 tctggcaaca tgcaaagcgg atgcttgctg attcctcact ggatatggtt ttgcttgatg
     7081 aactgacgta tatggtggcg tatgactatt tgccactgga agaagtggtg caggcgttaa
     7141 atgaacgtcc acatcaacag acggtgatta tcacgggtcg aggctgtcat cgggatattc
     7201 ttgaactggc ggatacggta agtgagctgc gccccatcaa acatgcgttt gatgccggtg
     7261 ttaaggcgca aatagggatt gattattaat ctgtaaaaat gccggatatg acgtccggca
     7321 tactttgatt aaccgttatt acgcccgcca gaacgacgac cgccgctcac ctgactgtga
     7381 cgtttcaccg cacgacgaat ctgattagcc ttcatgcgac gacggtcttt ttctacagcg
     7441 actttagagc tggtttctgg cggaagctcc accagttcgc gcagatagtt agtctgggcg
     7501 aggtccagct ccgtccagcc accgcgtggc agaccttttg gcagtgggat atcaccgtaa
     7561 cgaacacgga tcaggcggct cacctgcaca ccaaccgctt cccacagacg acgaacttca
     7621 cggttacggc cttcggtcag agtcacgttg taccactggt tgatcccttc gccgccgctg
     7681 aacttgatgg ttttaaaagc tgccggacca tcttccagtt gcacgccacg actcaaatca
     7741 cgcagtttcg cgtcgtcaac ctgaccaaat acacgcacgg cgtattcacg ttcaacttca
     7801 cggcttggat gcattaaacg gtttgccagt tcaccatcgg tggtgaacag cagcagacca
     7861 caggtgttaa cgtccagacg acctacggca atccagcgtg cgccacgcag ctttggcaga
     7921 cggtcaaaca ccgtcggacg cccttccgga tcgttacggg tacacaactc accttccggt
     7981 ttgtaatagg ccagaacgcg acaaatttgt tcagcggact cacgtaccga aatcagatga
     8041 ccatcgatac gaattttcag cccaggggta acttcaacgc gatcgccgag tttagcaatt
     8101 ttgccatcaa cactcacacg accggcttca ataatagatt cgatttcacg gcgggagccg
     8161 tggccagcgc gggccagcac tttctgtaat ttttcgctca tagagcttcc ttttggtgtc
     8221 gccttcccag gcgtctgata tgcttttgtg caacagtgag tcagaactta catctgactc
     8281 actgatagta aagtctgttt gaactcttca ggccttaagt aaacgactcg acgttttaac
     8341 tggcctggct ttgaggcgca acatactaca ctatctttat ggttaaagct tgtttcgatt
     8401 aacatcagct taattaccag ccaccaccgc ctccgccacc cgcgccgcca ccagcaccac
     8461 caccggaata acttcgcccc aacaaactgg ttttggcccc cagactcagg tgcatctgta
     8521 atgaaaacaa aacgccacta cgggcaattt ccggggcgct aacagccgct gtgagtgatg
     8581 gggtgatacg tgctacccat tctcttccaa ggtttgctga gactgcccaa cctagcaacg
     8641 cgtaatccag tttccgtgtt tcccctttcc gccgacgccc actgtgtgtt gctgcctctt
     8701 tacgcgcaag atagttaacg atagcttcac cgtgggcata acgttgctgc ccagtttgtg
     8761 taaactttgg cagaaaaata taccccatgc cagtgagata accggtcagg aaaacagcgc
     8821 taaaataacc cgctggcata taccagtaaa agaatacatc tccgacattg atgtagagag
     8881 aatagaaggc cacgcctgaa caaatcagtg gtaaacaatt cttccgtcct caaggatgcg
     8941 gccatttcga tatgtgtcgc cctcttctcc agtaaagaga tcgatttcgc taaatggatt
     9001 acccgcgata tcagggaact tgagtgaaaa tcgggtctta tagatttcaa acggccagtg
     9061 gttaccagtc acgttccaga ttaacagatc tgaatctccc tcacgcagga aagcattttt
     9121 aacctgataa tgaatttgat aatgatagtc gccttgaggc aagaaacgtt gtttatcgcc
     9181 cacgacaata ctcattaacc cgatatgcca gtcatgatgc cagggttcag gaataccatc
     9241 acgggtgacg ccaacaatat gatagttcag cagtgcagcg tccccatcag gctgcatcca
     9301 gagtcgtggt aaatcacgat aaaatccgtg tttaatctgc tgatttcgcg cctgaaaatg
     9361 aatattttct ttgatgtcga gcaccccatc cggttgcaga gtggcttcaa catcaaagag
     9421 cgaaatccct tcttcataag aagggatctc cgtgttttct gcataactta gagatgatac
     9481 aaatagccct gctatcaaca gcaaaatgca gcgaaacttc cctgccatag tcttccatcc
     9541 gtggaacgca atttgttata agaaaggctt cacatcacct acgccttcac gcaccacgac
     9601 cggcgtatca tcggtaagat caataaccgt tgtcggtttc tgcccgagat agccgccgtg
     9661 aataatcaaa tctacctgtt tttccagacg gtctttaatt tcttcgggat cggattcggt
     9721 aaattcgctg cctggcagca ttagcgaagt ggaaagcatc ggttcgccca gtgcttcaag
     9781 caacgcctgc gcaatcgggt tagaaggcac acgcataccg atggttttgc gtttttcctg
     9841 caacagacgg cgtggcactt ccttcgtccc cttcaggatg aaggtatagt tgcccggcgt
     9901 gttgtttttc attaaacgaa acgcaacgtt atcaacaaat gaataggtcg agagttcaga
     9961 aagatcgcga cacatcaggg taaagttgtg accgtccggc agctggcgaa tacgacaaat
    10021 acgctccatc gcgtttttat cttcaatttt acagccgagc gcatagccgg aatcagttgg
    10081 ataaacaatc accccgcctt tacgcacgat ctccaccgcc tggttgatca gacgttgctg
    10141 tgggttatca ggatgaatat aaaaaaactg gctcataatt ccctctctgt ggtgttctgc
    10201 ggctgttccc atagctgcca gacgccttca acgcctgcgg gcagccagag tttacgacct
    10261 agttcgatcc acgggcatgg ctgatgaaaa tcagatcctt gtgatgccca taaatgatgc
    10321 tgacgcgcaa gggccgccag ctgggtacgt tcattgggcg attgctggca ctgcgcgact
    10381 tccatcgcgt caccgtggtg ttcggcaaaa tgcgcaacca atcttttcag ccatttagca
    10441 ctaagattgt accgcccagg atgagctagc accgccttac cgccagaatg atgaatgaca
    10501 tcaatagctt gttctattgt acaccactgt ggcggaacgt atccggtttt cccgcgcgcc
    10561 agatattttt taaagacatc cgccattgaa ctggctttgc cgcaccccac aaggaaacgc
    10621 gcaaaatgac cgcgcgtcac cgcaccgccc tgcgccagtc gtcgcgcgcc ttccagtgcc
    10681 cctggtattt gcgctttttc aagtctttcg gcaatcagtt gcgcccgctg attgcgccgt
    10741 tctgtctgct gcgcgaggaa ctcacacatt agcggatgag taatatcaat attcagcccg
    10801 acaatatgaa tttcatgatt ttcccagacc gtggaaattt ccacgccggg aataagattc
    10861 agcgccagtc ccgaacgtga aatttcctct cttgctggca cgattgcagc tgtgttgtca
    10921 tggtcggtga tcgccagggt accgacgcgc atctcgactg cacggtgcac caatacttct
    10981 ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcgtaa
    11041 ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac
    11101 agttctggca aatattctga aatcagctgt tgacaattaa tcatcgaact agttaactag
    11161 tacgcaagtt cacgtaaaaa gggtatcgac aatgaaagca attttcgtac tgaaagcttg
    11221 gtggcgcact tcctgaaacg ggcagtgtat tcaccatgcg taaagcaatc agatacccag
    11281 cccgcctaat gagcgggctt ttttttgaac aaaattagag aataacaatg caaacacaaa
    11341 aaccgactct cgaactgcta acctgcgaag gcgcttatcg cgacaacccg actgcgcttt
    11401 ttcaccagtt gtgtgggaat cgtccggcaa cgctgctgct ggaatccgca gatatcgaca
    11461 gcaaagatga tttaaaaagc ctgctactgg tagacagtgc gctgcgcatt acagcattag
    11521 gtgacactgt cacaattcag gcactttccg gcaacggcga agccctgctg gcactactgg
    11581 ataacgccct gcctgcgggt gtggaaaatg aacaatcacc aaactgccgt gtgctgcgct
    11641 tcccccctgt cagtccactg ctggatgaag acgcccgctt atgctccctt tcggtttttg
    11701 acgctttccg tttattgcag aatctgttga atgtaccgaa ggaagaacga gaagcaatgt
    11761 tcttcggcgg cctgttctct tatgaccttg tggcgggatt tgaagattta ccgcaactgt
    11821 cagcggaaaa taactgccct gatttctgtt tttatctcgc tgaaacgctg atggtgattg
    11881 accatcagaa aaaaaacacc cgcattcagg ccagcctgtt tgctccgaat gaagaagaaa
    11941 aacaacgtct cactgctcgc ctgaacgatc ttcgccagca gctgaccgaa gccgcgccgc
    12001 cgctgccggt ggtttccgtg ccgcatatgc gttgtgaatg taatcagagc gatgaagagt
    12061 tcggtggcgt agtgcgtttg ttgcaaaaag cgattcgcgc tggagaaatt ttccaggtgg
    12121 tgccatctcg ccgtttctct ctgccctgcc cgtcaccgct ggcggcctat tacgtgctga
    12181 aaaagagtaa tcccagcccg tacatgtttt ttatgcagga taatgatttc accctgtttg
    12241 gcgcgtcgcc ggaaagttcg ctcaagtatg acgccaccag ccgccagatt gagatctacc
    12301 cgattgccgg aacacgtcca cgcggtcgtc gtgccgatgg ctcgctggac agagacctcg
    12361 acagccgcat cgaactggaa atgcgtaccg atcataaaga gctttctgaa catctgatgc
    12421 tggtggatct cgcccgtaat gatctggcac gcatttgcac ccccggcagc agctacgtcg
    12481 ccgatcttac caaagttgac cgttactctt acgtgatgca cctggtctcc cgcgtggtcg
    12541 gtgagctgcg ccacgatctc gacgccctgc acgcttaccg cgcctgtatg aatatgggga
    12601 cgttaagcgg tgcgccgaaa gtacgcgcca tgcagttaat tgccgaggcg gaaggtcgtc
    12661 gccgcggcag ctacggcggc gcggtaggtt atttcaccgc gcatggcgat ctcgacacct
    12721 gcattgtgat ccgctcggcg ctggtggaaa acggtatcgc caccgtgcaa gccggtgctg
    12781 gcgtagtcct tgattctatt ccgcagtcgg aagccgacga aacccgtaat aaagcccgcg
    12841 ctgtactgcg cgctattgcc accgcgcatc atgcacagga gactttctga tggctgacat
    12901 tctgctgctc gataatatcg actcttttac gtacaacctg gcagatcagt tgcgcagcaa
    12961 tgggcataat gtggtgattt accgcaacca tattccggcg cagaccttaa ttgaacgcct
    13021 ggcgacgatg agcaatccga tactgatgct ttctcctggc cccggtgtgc cgagcgaagc
    13081 cggttgtatg ccggaactcc tcacccgctt gcgtggcaag ctgcccatta ttggcatttg
    13141 cctcggacat caggcaattg tcgaagctta cgggggctat gtcggtcagg cgggcgaaat
    13201 tcttcacggt aaagcgtcga gcattgaaca tgacggtcag gcgatgtttg ccggattaac
    13261 aaacccgctg ccggtggcgc gttatcactc gctggttggc agtaacattc cggccggttt
    13321 aaccatcaac gcccatttta atggcatggt gatggcagta cgtcacgatg cggatcgcgt
    13381 ttgtggattc cagttccatc cggaatccat tctcaccacc cagggcgctc gcctgctgga
    13441 acaaacgctg gcctgggcgc agcagaaact agagccagcc aacacgctgc aaccgattct
    13501 ggaaaaactg tatcaggcgc agacgcttag ccaacaagaa agccaccagc tgttttcagc
    13561 ggtggtgcgt ggcgagctga agccggaaca actggcggcg gcgctggtga gcatgaaaat
    13621 tcgcggtgag cacccgaacg agatcgccgg ggcagcaacc gcgctactgg aaaacgcagc
    13681 gccgttcccg cgcccagatt atctgtttgc tgatatcgtc ggtactggcg gtgacggcag
    13741 caacagtatc aatatttcta ccgccagtgc gtttgtcgcc gcggcctgtg ggctgaaagt
    13801 ggcgaaacac ggcaaccgta gcgtctccag taaatctggt tcgtccgatc tgctggcggc
    13861 gttcggtatt aatcttgata tgaacgccga taaatcgcgc caggcgctgg atgagttagg
    13921 tgtttgtttc ctctttgcgc cgaagtatca caccggattc cgccacgcga tgccggttcg
    13981 ccagcaactg aaaacccgca ccctgttcaa tgtgctgggg ccattgatta acccggcgca
    14041 tccgccgctg gcgttaattg gtgtttatag tccggaactg gtgctgccga ttgccgaaac
    14101 cttgcgcgtg ctggggtatc aacgcgcggc ggtggtgcac agcggcggga tggatgaagt
    14161 ttcattacac gcgccgacaa tcgttgccga gctgcatgac ggcgaaatta agagctatca
    14221 attgaccgct gaagattttg gcctgactcc ctaccaccag gagcaactgg caggcggaac
    14281 accggaagaa aaccgtgaca ttttaacacg cttgttacaa ggtaaaggcg acgccgccca
    14341 tgaagcagcc gtcgctgcga acgtcgccat gttaatgcgc ctgcatggcc atgaagatct
    14401 gcaagccaat gcgcaaaccg ttcttgaggt actgcgcagt ggttccgctt acgacagagt
    14461 taccgcgctg gcggcacgag ggtaaatgat gcaaaccgtt ttagcgaaaa tcgtcgcaga
    14521 caaggcgatt tgggtagaaa cccgcaaaca gcagcaaccg ctggccagtt ttcagaatga
    14581 ggttcagccg agcacgcgac atttttatga tgcgctacag ggtgcacgca cggcgtttat
    14641 tctggagtgc aagaaagcgt cgccgtcaaa aggcgtgatc cgtgatgatt tcgatccggc
    14701 acgcattgcc tccatttata aacattacgc ttcggcaatt tcggtgctga ctgatgagaa
    14761 atattttcag gggagctttg atttcctccc catcgtcagc caaatcgccc cgcagccgat
    14821 tttatgtaaa gacttcatta tcgaccctta ccagatctat ctggcgcgct attaccaggc
    14881 cgatgcctgc ttattaatgc tttcagtact ggatgacgac caatatcgcc agcttgcagc
    14941 cgtcgctcac agtctggaga tgggggtgct gaccgaagtc agtaatgaag aggaactgga
    15001 gcgcgccatt gcattggggg caaaggtcgt tggcatcaac aaccgcgatc tgcgtgattt
    15061 gtcgattgat ctcaaccgta cccgcgagct tgcgccgaaa ctggggcaca acgtgacggt
    15121 aatcagcgaa tccggcatca atacttacgc tcaggtgcgc gagttaagcc acttcgctaa
    15181 cggttttctg attggttcgg cgttgatggc ccatgacgat ttgcacgccg ccgtgcgccg
    15241 ggtgttgctg ggtgagaata aagtatgtgg tctgacgcgt gggcaagatg ctaaagcagc
    15301 ttatgacgcg ggcgcgattt acggtgggtt gattttggtt gcgacatcac cgcgttgcgt
    15361 caacgttgaa caggcgcagg aagtgatggc tgcggcaccg ttgcagtatg ttggcgtgtt
    15421 ccgcaatcac gatattgccg atgtggtgga caaagctaag gtgttatcgc tggcggcagt
    15481 gcaactgcat ggtaatgaag atcagctgta tatcgatacg ctgcgtgaag ctctgccagc
    15541 acacgttgcc atctggaaag cattaagcgt cggtgaaacc ctgcccgccc gcgagcttca
    15601 gcacgttgat aaatatgttt tagacaacgg ccagggtgga agcgggcaac gtttcgactg
    15661 gtcactatta aatggtcaat cgcttggcaa cgttctgctg gcggggggct taggcgcaga
    15721 taactgcgtg gaagcggcac aaaccggctg cgccggactt gattttaatt ctgctgtaga
    15781 gtcgcaaccg ggcatcaaag acgcacgtct tttggcctcg gttttccaga cgctgcgcgc
    15841 atattaagga aaggaacaat gacaacatta cttaacccct attttggtga gtttggcggc
    15901 atgtacgtgc cacaaatcct gatgcctgct ctgcgccagc tggaagaagc ttttgtcagc
    15961 gcgcaaaaag atcctgaatt tcaggcccag ttcaacgacc tactgaaaaa ctatgccggg
    16021 cgtccaaccg cactgaccaa atgccagaac attacggctg gcacgaacac cacgctgtat
    16081 ctgaagcgcg aagatttgct gcacggcggc gcgcataaaa ctaaccaggt gctcggtcag
    16141 gctttactgg cgaagcggat gggtaaaacc gaaatcatcg ccgaaaccgg tgccggtcag
    16201 catggcgtgg cgtcggccct tgccagcgcc ctgctcggcc tgaaatgccg tatttatatg
    16261 ggtgccaaag acgttgaacg ccagtcgcct aacgtttttc gtatgcgctt aatgggtgcg
    16321 gaagtgatcc cggtgcatag cggttccgcg acgctgaaag atgcctgtaa cgaggcgctg
    16381 cgcgactggt ccggtagtta cgaaaccgcg cactatatgc tgggcaccgc agctggcccg
    16441 catccttatc cgaccattgt gcgtgagttt cagcggatga ttggcgaaga aaccaaagcg
    16501 cagattctgg aaagagaagg tcgcctgccg gatgccgtta tcgcctgtgt tggcggcggt
    16561 tcgaatgcca tcggcatgtt tgctgatttc atcaatgaaa ccaacgtcgg cctgattggt
    16621 gtggagccag gtggtcacgg tatcgaaact ggcgagcacg gcgcaccgct aaaacatggt
    16681 cgcgtgggta tctatttcgg tatgaaagcg ccgatgatgc aaaccgaaga cgggcagatt
    16741 gaagaatctt actccatctc cgccggattg gatttcccgt ctgtcggccc acaacacgcg
    16801 tatcttaaca gcactggacg cgctgattac gtgtctatta ccgatgatga agcccttgaa
    16861 gccttcaaga cgctgtgcct acacgaaggg atcatcccgg cgctggaatc ctcccacgcc
    16921 ctggcccatg cgctgaaaat gatgcgcgaa aacccggaaa aagagcagct actggtggtt
    16981 aacctttccg gtcgcggcga taaagacatc ttcaccgttc acgatatttt gaaagcacga
    17041 ggggaaatct gatggaacgc tacgaatctc tgtttgccca gttgaaggag cgcaaagaag
    17101 gcgcattcgt tcctttcgtc acgctcgggg atccgggcat tgagcagtca ttgaaaatta
    17161 tcgatacgct aattgaagcc ggtgctgacg cgctggagtt aggcatcccc ttctccgacc
    17221 cactggcaga tggcccgacg attcaaaacg ccactctgcg cgcctttgcg gcaggtgtga
    17281 ctccggcaca atgttttgaa atgctggcac tgattcgcca gaaacacccg accattccca
    17341 ttggcctgtt gatgtatgcc aatctggtgt ttaacaaagg cattgatgag ttttatgccc
    17401 agtgcgagaa agtcggcgtc gattcggtgc tggttgccga tgtgccaatt gaagagtccg
    17461 cgcccttccg ccaggccgcg ttgcgccata atgtcgcacc tatcttcatc tgcccgccaa
    17521 atgccgatga cgacctgctg cgccagatag cctcttacgg tcgtggttac acctatttgc
    17581 tgtcacgagc gggcgtgacc ggcgcagaaa accgcgccgc gttacccctc aatcatctgg
    17641 ttgcgaagct gaaagagtac aacgctgcac ctccattgca ggggtttggt atttccaccc
    17701 cggatcaggt aaaagcagcg attgatgcag gagctgcggg cgcgatttct ggttcagcca
    17761 ttgttaaaat catcgagcaa catattaatg agccagagaa aatgctggcg gcactgaaag
    17821 tttttgtaca accgatgaaa gtggcgacgc gcagttaatc ccccagccgc cagttccgct
    17881 ggcggcattt taactttctt taacgaagcc ggaaaaatcc taaattcatt taatatttat
    17941 ctttttaccg tttcgc
//
LOCUS       DAZPLI010000077        17899 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZPLI010000077 DAZPLI010000000
VERSION     DAZPLI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17899
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.078"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            183..861
                     /locus_tag="ACHUU3_002503"
                     /pseudo
     CDS             183..861
                     /locus_tag="ACHUU3_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            905..5755
                     /locus_tag="ACHUU3_002504"
     CDS             905..5755
                     /locus_tag="ACHUU3_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFR2082533.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKTLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6123..7778
                     /gene="lldP"
                     /locus_tag="ACHUU3_002505"
     CDS             6123..7778
                     /gene="lldP"
                     /locus_tag="ACHUU3_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR2082534.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7778..8554
                     /gene="lldR"
                     /locus_tag="ACHUU3_002506"
     CDS             7778..8554
                     /gene="lldR"
                     /locus_tag="ACHUU3_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR2082535.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8551..9741
                     /gene="lldD"
                     /locus_tag="ACHUU3_002507"
     CDS             8551..9741
                     /gene="lldD"
                     /locus_tag="ACHUU3_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR2082536.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9925..10398
                     /gene="trmL"
                     /locus_tag="ACHUU3_002508"
     CDS             9925..10398
                     /gene="trmL"
                     /locus_tag="ACHUU3_002508"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR2082537.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10451..11272)
                     /gene="cysE"
                     /locus_tag="ACHUU3_002509"
     CDS             complement(10451..11272)
                     /gene="cysE"
                     /locus_tag="ACHUU3_002509"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR2082538.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11352..12371)
                     /gene="gpsA"
                     /locus_tag="ACHUU3_002510"
     CDS             complement(11352..12371)
                     /gene="gpsA"
                     /locus_tag="ACHUU3_002510"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR2082539.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12371..12838)
                     /gene="secB"
                     /locus_tag="ACHUU3_002511"
     CDS             complement(12371..12838)
                     /gene="secB"
                     /locus_tag="ACHUU3_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR2082540.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12901..13152)
                     /gene="grxC"
                     /locus_tag="ACHUU3_002512"
     CDS             complement(12901..13152)
                     /gene="grxC"
                     /locus_tag="ACHUU3_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR2082541.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13294..13725)
                     /locus_tag="ACHUU3_002513"
     CDS             complement(13294..13725)
                     /locus_tag="ACHUU3_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR2082542.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13970..15514
                     /gene="gpmM"
                     /locus_tag="ACHUU3_002514"
                     /gene_synonym="pgmI"
     CDS             13970..15514
                     /gene="gpmM"
                     /locus_tag="ACHUU3_002514"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR2082543.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15548..16807
                     /gene="envC"
                     /locus_tag="ACHUU3_002515"
     CDS             15548..16807
                     /gene="envC"
                     /locus_tag="ACHUU3_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR2082544.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16811..17770
                     /locus_tag="ACHUU3_002516"
     CDS             16811..17770
                     /locus_tag="ACHUU3_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR2082545.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(17757..>17899)
                     /locus_tag="ACHUU3_002517"
     CDS             complement(17757..>17899)
                     /locus_tag="ACHUU3_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR2082546.1"
                     /translation="MYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHHRITRSAFN
                     LR"
BASE COUNT         4496 a         4629 c         4676 g         4098 t
ORIGIN      
        1 atatattcat acaaacacag acatcttttg aaacgaatga tacattttta tgatgaatgc
       61 ttaaaacaat atttaatcga cacacatcac tgtcattaat gtaaagcaac atagatcacc
      121 aataatacaa tggacaactc taccggcatt gatcgctggt cggaggaaat aaggattttt
      181 acatgaactt gaagaagatt tttttcagtg ctgtcactgt ttcagttctc tgcgcattaa
      241 cgggatgtga ctacattgaa gaaggcaagc cagaaagtag tttattgaaa caacaagaag
      301 aacattaaaa ttgatctact tgaaaaacaa caagcacagc taaagagtca actggaaact
      361 attcaaaaac aacaaactgg cataataagc agtacaaaga ctttaactca cgtaattaaa
      421 tccgttaaag atcaacaaaa cacttttatt tttaccgagt ttaacccagc aaaaacgaag
      481 tatttcatcc tgaacaatgg ttcggtggct ttagcgggtc gagtgttatc cattgacgcc
      541 acagagaatg gcagtgttat tcatatttca ctggtcaact tattaagtac acctatctca
      601 aatattggtt ttaatgcgac atggggtggt gaaaaacctg tcgatgccaa agagtttgcc
      661 cggtggcaac aattactttt caacacatca atgaaatcca cattgaaatt attaccaggt
      721 caatggcaag acattaattt gaccctgaag ggtgtatcgc ccaataacct gggatatctg
      781 aagttagcca tcaacatgga aaatattcag ttcgacaatc ttccctctgc tgaaaatcgg
      841 cagaaaagaa gcaaaaaata aatgcagtta gtacgcttat aacaaggaat agcgagcatc
      901 attaatgaac aaaatattta aagttatctg gaaccctgcg acagggaatt atactgttac
      961 cagcgaaacg gcaaaaagcc gtggcaagaa atctgggcgc agtaagctgt taatttctgc
     1021 gctggttgcg ggtggaatgt tgtcgtcgtt tggggcattg gcgaatgccg ggaatgacaa
     1081 cggccagggt gttgattacg gtagtggatc agctggcgac agctgggttg ctataggcaa
     1141 aggggcgaaa gcaaatactt ttatgaacac cagtggttcc agtactgctg tgggttatga
     1201 cgctatagct gaaggccaat atagctctgc catcgggtca aaaacccatg cgattggtgg
     1261 tgcatcaatg gcctttgggg ttagtgcaat atcagaaggc gatagaagta tagcactggg
     1321 tgcctcttcg tattcattgg gccaatactc aatggccctc ggccgttatt caaaagcatt
     1381 gggtaaattg tctattgcta tgggggactc ttccaaagcg gaaggagcaa acgccattgc
     1441 cctgggaaat gccactaaag ctactgagat tatgagtatt gctcttggcg acaccgccaa
     1501 tgcgtcaaaa gcgtattcaa tggcgctggg agcaagtagc gtcgcatctg aagaaaacgc
     1561 aattgccctg gggcgtagca gtgtagctag cggtactgac agcctcgcat ttggcagaca
     1621 atcacttgcc agcgcagcga acgctattgc gataggtgct gagaccgaag ccgctgaaaa
     1681 tgcaactgct attggcaata atgcgaaggc aaaagggact aatagcatgg caatggggtt
     1741 cggaagcctt gccgataaag tcaatactat cgcattagga aatggcagcc aggctctggc
     1801 agataatgca atcgccatag gccagggcaa caaagctgat ggcgtggatg ccatcgctct
     1861 gggtaatggt agccagtcga gaggcttaaa caccattgcc ttaggcacag ccagtaatgc
     1921 aactggtgat aagagtcttg cgcttggtag taatagcagt gccaacggta ttaactctgt
     1981 cgcgctgggc gcagattcca ttgcggattt agacaatacc gtctctgtcg gcaatagttc
     2041 attaaaacgc aagatcgtta atgtgaaaaa tggcgcgatc aagtctgaca gttacgatgc
     2101 cattaatggt tcacagcttt atgccattag cgactcggta gcaaaaacgc ttggaggagg
     2161 ggctgcagta gatgttgatg acggtactgt tacagcacca acctacaatt taaaaaatgg
     2221 tagcaaaaat aacgtagggg ctgcgctcgc tgtacttgat gaaaacaccc tgcaatggga
     2281 ccaaaccaaa ggcaaataca gcgctgctca tggtactagt agcccaactg ccagcgtaat
     2341 caccgatgtt gcggatggca cgatttcagc ctccagtaag gatgcggtta acggttccca
     2401 actgaaagct accaatgacg atgtcgaagc caacaccgcc aatatcgcta ctaataccag
     2461 caacattgcc acgaatacag caagtattgc caccaatacc accaatatca ccaacctgac
     2521 ggattccgtt ggtgaccttc aggctgatgc cctgctctgg aacgaaacta aaaaggcatt
     2581 cagtgcagct cacggccagg ataccaccag caaaatcacc aacgttaaag atgccgacct
     2641 gacggctgac agcactgatg ctgttaacgg ctctcagctg aaaaccacca acgatgctgt
     2701 ggcgacgaat accaccaata tcgccaataa cacttccaat attgccacta acaccaccaa
     2761 catctctaat ctgactgaga cggtgactaa tcttggtgag gatgcgctga aatgggataa
     2821 ggacaatggt gtattcacgg cagctcatgg caccgagacc accagcaaaa tcaccaacgt
     2881 taaagatggc gacctgacga ctggcagcac cgatgccgtt aacggctctc agctgaaaac
     2941 caccaacgat gccgtggcga cgaataccac caatatcgcc actaacacca ccaacatctc
     3001 taatctgact gagacggtga ctaatcttgg tgaggatgcg ctgaaatggg ataaggacaa
     3061 tggtgtcttc actgcagctc atggcaacaa taccgccagc aaaatcacca atatcctgga
     3121 cggcacagtc actgcaacca gttccgatgc cattaacggt agccagcttt atgacttaag
     3181 cagcaatatc gccacctact tcggcggcaa tgcttctgtg aatactgacg gtgtgtttac
     3241 cggtccaacc tacaaaatcg gtgaaacaaa ttattataac gtcggcgatg cactggctgc
     3301 gattaactcc tcatttagca cgtctctcgg cgatgctctg ctttgggatg ccaccgcagg
     3361 taaattcagt gccaaacacg gtactaatgg tgacgcaagc gtgatcactg atgtcgcaga
     3421 tggtgaaatt tcagactcca gttctgacgc agtaaacggc tcacaactcc acggcgtgag
     3481 cagttatgtt gttgatgcgc tggggggtgg tgccgaagtc aatgcagacg gcaccatcac
     3541 tgcgccgacg tacaccattg ctaatgctga ttacgataat gtcggtgatg ccctgaatgc
     3601 tatcgatacc actcttgacg acgctctgct ctgggatgcg gacgccggtg aaaatggtgc
     3661 atttagcgcc gctcacggaa aagataaaac tgccagtgta atcactaacg tcgctaacgg
     3721 tgcaatctct gctgccagca gcgacgcgat taacggctca caactctata ccaccaataa
     3781 gtacatcgct gatgcgctgg gtggtgacgc agaagtcaac gctgacggca ccatcaccgc
     3841 accgacttac accattgcga acgccgagta caacaacgtc ggtgacgccc tggatgcgct
     3901 tgatgataac gccctgctgt gggatgagac tgccaatggc ggtgctggag cctacaatgc
     3961 cagccatgac ggtaaagcca gcatcatcac taatgtcgct aatggcagta ttagtgagga
     4021 cagtaccgat gcagtgaacg gttctcagtt gaatgcgacg aatatgatga ttgagcagaa
     4081 cacccaaatt atcaatcagc tcgctggtaa caccgacgca acctatatcg aagaaaatgg
     4141 cgctggtatc aactacgttc gtactaacga caacgattta gcctttaacg atgcaagcgc
     4201 ctctggtgtc ggcgctacag ctgtaggtta taacgctgtc gcgtctggtg ccagcagcgt
     4261 agccattggt cagaacagca gcagcaccgt tgataccggt attgcgctgg gtagcagctc
     4321 cgtttccagc cgtgtgattg ccaaaggttc tcgtgacact agcgtaacgg aaaatggcgt
     4381 ggtgattggt tatgacacca ctgacggcga actgctaggt gcattgtcaa ttggtgatga
     4441 cggtaaatac cgccaaatca tcaacgtagc tgatggttca gaagcccatg acgccgttac
     4501 ggttcgccag ttgcagaacg ctattggagc ggtcgccact acgccaacca agtactttca
     4561 cgccaactca acggcagaag actcactggc cgttggtgaa gactcgctgg caatgggtgc
     4621 gaaaactgtc gttaatggta atgcaggtat tggtatcggt ttgaacactc tggttctagc
     4681 tgatgcgatc aacggcattg ctatcggcag taacgcacgc gcaaaccatg caaacagtat
     4741 cgcaatgggt aacggttctc agaccacccg tggtgcacag actggctaca ccgcctacaa
     4801 catggacgca ccgcagaact ctgtaggtga gttttctgtc ggtagtgaag acggtcaacg
     4861 tcagatcaca aacgtcgcag ctggttcagc ggataccgat gcggttaacg tgggtcagtt
     4921 gaaagtcact gatgagcgcg tagcgcaaaa tacccagagc attactaacc tgaacaatca
     4981 ggtcactaat ctggatactc gcgttactaa tatcgaaaac ggtattggcg acattgtcac
     5041 caccggtagc accaagtact tcaagaccaa caccgatggc gtagatgcca acgcccaggg
     5101 taaagatagc gttgctattg gttctggttc cattgctgcc gctgacaaca gcgtcgcact
     5161 gggtaccggt tccgttgcag aggaagaaaa tacaatctct gtaggttctt ctactaacca
     5221 acgccgtatc accaacgtag ctgccggtgt taatgccacc gatgcggtta acgtgtcgca
     5281 gctgaaatct tctgaagcag gcggcgtgcg ctacgacacc aaagctgatg gttctatcga
     5341 ctatagcaat atcaccctcg gtggtggcaa cggcagtacg actcgtatca gcaacgtctc
     5401 cgctggcgtc aacaacaacg acgcggtgaa ctacgcgcag ttgaagcaaa gcgtgcagga
     5461 aacgaagcaa tacaccgatc agcggatggt tgagatggat aacaaactgt ctaaaactga
     5521 aagcaagttg agcggtggta tcgcttctgc aatggcaatg accggtctgc cgcaggctta
     5581 tacaccgggt gccagcatgg cttctattgg tggcggtact tacaacggtg aatcggcagt
     5641 tgctttaggt gtatcgatgg tgagcgccaa tggtcgttgg gtctacaaat tacaaggtag
     5701 taccaatagc cagggtgaat actccgccgc actcggtgcc ggtattcagt ggtaatgatc
     5761 cattaagtta gtgtgactaa gggcaaacgc caaattgcct gatgcgttac gcgcatcagg
     5821 caagataagg cgccccgggt caacaatcta agggcaattc tctgatgagg attgcccttt
     5881 tctttaccag acatctcccc ccacaagaat tggccctacc aattcttcgc ttatctgacc
     5941 tctggttcac aattccccaa ttaaaactca catcaatatt gccaatacat aacatttagt
     6001 taaccattca ttgtcattat ccctacacaa cacaattggc agtgccactt ttacacaacg
     6061 tgtgacaagg agatgagcaa cagactcatt acacgatgtg cgtggactcc aggagacctg
     6121 caatgaatct ctggcaacaa aactacgatc ccgccgggaa tatctggctt tccagtctga
     6181 tagcatcgct tcccatcctg tttttcttct ttgcgctgat taagctcaaa ctgaaaggat
     6241 acgtcgccgc ctcatggacg gtagcaatcg cccttgccgt ggctttgctg ttctataaaa
     6301 tgccggtcgc taacgcgctg gcctcggtgg tttatggctt cttctatggt ttgtggccca
     6361 tcgcgtggat cattattgca gcagtgttcg tctataagat ctcggtgaaa accgggcagt
     6421 ttgacatcat tcgctcgtct attctttcga taacccctga ccagcgtctg caaatgctga
     6481 tcgtcggttt ctgtttcggc gctttccttg aaggagccgc aggctttggc gcaccggtag
     6541 caattaccgc cgcattgctg gtcggcctgg gttttaaacc gctgtacgcc gccggactgt
     6601 gcctgattgt taacaccgcg ccagtggcat ttggcgcgat gggcattcca atcctggttg
     6661 ccggacaagt aacaggcatc gacagctttg agattggcca gatggtgggg cggcagctac
     6721 cgtttatgac cattatcgtg ctgttctgga tcatggcgat tatggacggc tggcgcggta
     6781 tcaaggagac gtggcctgcg gtcgtggttg cgggcggctc gtttgccatc gctcagtacc
     6841 ttagctctaa cttcattggg ccggagctgc cggacattat ctcttcgctg gtatcactgc
     6901 tctgcctgac gctgttcctc aaacgctggc agccagtgcg cgtattccgt tttggtgatt
     6961 tgggggcgtc aaagattgat atgacgctgg cccacaccgg ttacactgcg ggtcaggtgt
     7021 tacgtgcctg gacaccgttc ctgttcctga cagctaccgt aacactgtgg agcatcccgc
     7081 cgtttaaagc cctgttcgca tcgggtggtg cgctgtatga gtgggtgatc aacattccgg
     7141 tgccgtacct cgataaactg gttgcccgta tgccaccagt ggtcagcgag gctacagcct
     7201 atgccgccgt gtttaagttt gactggttct ctgccaccgg caccgccatt ctgtttgctg
     7261 cactgctctc gattgtctgg ctgaagatga aaccgtctga cgctatcagc accttcggca
     7321 gcacgctgaa agaactggct ctacccatct actccatcgg catggtgctg gcgttcgcct
     7381 ttatctcgaa ctattccgga ctgtcatcaa cactggcact ggcactggcg cacaccggtc
     7441 atgcattcac cttcttctcg ccgttcctcg gctggctggg ggtattcctg accgggtcgg
     7501 atacctcatc taacgccctg ttcgccgcgc tgcaagccac cgcagcacaa caaattggcg
     7561 tctctgatct gttgctggtt gccgccaata ccaccggtgg cgtcaccggt aagatgatct
     7621 ccccgcaatc tatcgctatc gcctgtgcgg cggtaggcct ggtgggcaaa gagtctgatt
     7681 tgttccgctt tactgtcaaa cacagcctga tcttcacctg tatggtgggc gtgatcacca
     7741 cgcttcaggc ttatgtctta acgtggatga ttccttaatg attgttttac ccagacgcct
     7801 gtcagacgag gttgccgatc gtgtgcgggc gctgattgat gaaaaaaacc tggaagcggg
     7861 catgaagttg cccgctgagc gccaactggc gatgcaactc ggcgtatcac gtaattcact
     7921 gcgcgaggcg ctggcaaaac tggtgagcga aggcgtgctg ctcagtcgac gcggcggcgg
     7981 gacgtttatt cgctggcgtc atgacacatg gtcggagcaa aacatcgtcc agccgctaaa
     8041 aacgctgatg gccgatgatc cggattacag tttcgatatt ctggaagccc gctacgccat
     8101 tgaagccagc accgcatggc atgcggcaat gcgcgccaca cctggcgaca aagaaaagat
     8161 tcagctttgc tttgaagcaa cgctaagtga agacccggat atcgcctcac aagcggacgt
     8221 tcgttttcat ctggcgattg ccgaagcctc acataacatc gtgctgctgc aaaccatgcg
     8281 cggtttcttc gatgtcctgc aatcctcagt gaagcatagc cgtcagcgga tgtatctggt
     8341 gccaccggtt ttttcacaac tgaccgaaca acatcaggct gtcattgacg ccatttttgc
     8401 tggtgatgcc gacggggcgc gtaaagcaat gatggcgcac cttagttttg ttcacaccac
     8461 catgaaacga ttcgatgaag atcaggctcg ccacgcacgg attacccgcc tgcccggtga
     8521 gcataatgag cattcgaggg agaaaaacgc atgattattt ccgcagccag cgattatcgc
     8581 gccgcagcgc aacgcattct gccgccgttc ctgttccact atatggatgg tggtgcatat
     8641 tctgaataca cgctgcgccg caacgtggaa gatttgtcag aagtggcact gcgccagcgt
     8701 attctgaaaa acatgtccga cttaagcctg gaaacgacgc tgtttaatga gaaattgtcg
     8761 atgccggtag cactggcacc ggtgggtttg tgtggcatgt atgcgcgtcg tggcgaagtt
     8821 caggcagcca aagcggcgga cgcgcatggc attccgttta ctctctcgac ggtttccgtt
     8881 tgcccgattg aagaagtcgc gccagccatc aagcgcccaa tgtggttcca gctttatgta
     8941 ctgcgcgatc gcggctttat gcgtaacgcg ctggagcgag caaaagcagc gggttgttcg
     9001 acgctggttt tcaccgtgga tatgccgaca ccgggcgcac gctaccgtga tgcgcattca
     9061 ggtatgagcg gcccaaatgc ggcaatgcgc cgctacttgc aggcggtgac gcatccgcaa
     9121 tgggcgtggg atgtgggcct gaacggtcgt ccacatgatt taggtaatat ctcagcttat
     9181 ctcggcaaac cgaccggact ggaagattac atcggctggc tggggaataa cttcgatccg
     9241 tccatctcat ggaaagacct tgagtggatc cgcgatttct gggatggccc gatggtgatt
     9301 aaagggatcc tcgatccgga agatgcgcgc gatgcagtac gttttggtgc tgatggaatt
     9361 gtggtttcta accacggtgg ccgccagctg gacggtgtac tctcttccgc tcgtgcgttg
     9421 cccgctattg cagatgcggt gaaaggtgat atcgccattc tggcggatag cggaattcgt
     9481 aacgggcttg atgtcgtgcg tatgattgcg ctcggtgccg acaccgtact gctgggtcgt
     9541 gctttcctgt atgcgctggc aacagcgggc caggcgggtg tagctaacct gctaaatctg
     9601 atcgaaaaag agatgaaagt ggcgatgacg ctgactggcg cgaaatcgat cagtgaaatt
     9661 acgcaagatt cgctggtgca ggggctgggt aaagagttgc ctacggcact ggctccgatg
     9721 gcgaaaggga atgcggcata gtcgtttgcg ccccctcacc ctcagggaga ggggaccgtt
     9781 cggcgctgta tgtactccct cactctgaaa cgacaccgca ctcttttttt ctccctcgcc
     9841 cctccgggga gggccggggt gaggggaaaa ggccgcacgg ttcaccaatt ttctgctatc
     9901 ctgcccccgc actaaggggg cagtatgcta aacatcgtac tttacgaacc agaaattccg
     9961 ccaaatactg gcaacatcat ccgtctttgc gctaataccg gctttcgtct gcatatcatc
    10021 gaaccgatgg gatttgcctg ggacgataag cgcctgcgcc gcgcggggct ggactatcac
    10081 gagtttaccg ccgttacgcg tcatcatgac tatcgcgcgt tcctcgaagc agaaaatccc
    10141 cagcgcctgt ttgccctcac cacgaaaggt acgcctgctc acagcgccgt aagctatcag
    10201 gatggcgatt atctgatgtt cggcccggaa acacgcggcc tgccagcgag cattcttgat
    10261 gccctgcccg ctgaacaaaa aattcgcatt ccgatggtgc cggacagccg cagcatgaat
    10321 ctgtccaatg cggtgtcggt agtggtgtat gaagcctggc ggcagctggg atatccggga
    10381 gcgttgttga gagattagtt actgtatgcc ggatagatga ttacatcgca tccggcacga
    10441 tcacaggaca ttagatccca tccccatact caaatgtatg gttaataccg ttgaaatgct
    10501 ggtccatatc cattgatggc ttatcgctgt ctggtttacc gacaatacga gccggaacgc
    10561 cagcggcggt tgtatgcggc ggcaccggtt gcagcaccac ggaacctgcg ccaatcttcg
    10621 cgccgcgacc aacttcaata ttgccgagga ttttcgcgcc cgcgccaatc atcacacctt
    10681 cacgaatttt cgggtgacgg tcaccaccag atttacccgt accgccaagc gtcacagatt
    10741 gcagaatcga tacgtcgttt tcaattaccg ccgtttcacc cacaacgatg cccgtggcgt
    10801 ggtcgagcat aatgccacgg ccaatttttg ctgccgggtg aatatcgacc tggaacgtca
    10861 cagaaacctg gttttgcaga aagattgcca atgcgcgacg cccctgattc cacaaccagt
    10921 gaccgatgcg ataggcctgc aaggcatgaa aacccttcag gtataacaac ggggttgagt
    10981 atttatcgac tgccgggtcg cgggtacgca ccgcctgaat atcacaggcc gcagaggcga
    11041 tcatttccgg gtcagcggcg taggcttctt ccaccacttc acggatagca atagcaggca
    11101 taattggcga tgacagcttg ttcgccagca tgtagctcag tgcactacca aggttttcgt
    11161 gcttgagtag cgtcgcgtgg taaaaactgg ccagcattgg ctcacagtcc gccagcgttc
    11221 tggcttcggc tttaatattg ttccagacaa tttccagttc ttcacacgac attgcttact
    11281 ccacacgatg agataatgac cggcccgttc tgcgcgggcc gggtcatagc ggtaacaaag
    11341 gttccctggg gttagtggct gctgcgctcg tccttgcgtg cacgacctaa taacgtcaat
    11401 gctgcctcgc gcgcgttttt tccgcaatat aatacttgat aaatttcctc ggttattggc
    11461 atttctacgc cgaagcgatg cgccagttcg cggacttctt tcgtattgcg gtagccttcc
    11521 accacctgac caatcttctc ctgcgcgctt tgtacatcca tgccctgacc gagcatcatg
    11581 ccaaaacggc ggttacgcga ctggttgtcg gtacaggtaa gcaccagatc gcccagcccc
    11641 gccatgccca taaaggtggc aggatcggca cccagcgccg cgccaagacg cgacatttca
    11701 gccagcccac gggtgatcag tgccgtacgc gcattcgcac caaaaccgat accgtcggac
    11761 atccccgcgc caatggcaat gacgtttttc accgcaccgc caagctgcac accgatgaaa
    11821 tccgggttgc tgtaaacgcg gaaacttttg ccacagtgca gcaattgttg gagatcatcg
    11881 gcaaaggtct ggtcggtaga ggccaacgaa atagccgtcg gtaaacctgc cgccagttct
    11941 ttcgcaaacg ttgggccaga gataaccgcc agcggaattt gatcgcctaa ggcctcacgc
    12001 gccacgtcct gtaacagacg tccggtttcc gcttccagcc ctttggtcgc ccacaccaga
    12061 cgcgcatcag gacgcatcag cggtttaatc tggcgcagca cttcaccaaa gacatggctg
    12121 ggtacgacga cgagaatatt acggctggct gccagcgcag tggcgagatc gctttcaaga
    12181 tggagcgtat cgggaaaagg cacatcgggg agaaacgcgg cgttacagcg gtcgcgttca
    12241 agcgttgcga tatgttcagg gtcatggccc cagaggacaa cctcgtggcc atttcttgcc
    12301 agggtaatgg caagagcggt gccgtacgag ccggcaccga tcacagtcat tgaagcatta
    12361 cgttggttca tcaggcatcc tgatgttctt cagtaccttc gccagcctgc tgctgcaaat
    12421 agttcatgaa cagcgcatcg aagttaatcg gcgcaaggtt cagttgcggg aatgtaccgc
    12481 gggataccac gctggtgatg cactcacgag catacgggaa cagaatgttc gggcagtatg
    12541 cgcccaggca atgtgccatc tgggtgcctt cgatacccgc gatggagaaa ataccgccct
    12601 gctgaacttc acacaggaac gcggtttctt cgcccaggga ggccgttacg gtaacacgca
    12661 gtaccacttc gtatacgtca tctgccagtt gggaagatgc cgtatccaga tcaagtttaa
    12721 cttctggttg ccaatctttc tggaaaacgt gcggcgcgtt cggcgcttcg aaagagatat
    12781 ccttggtata aatacgttgg atctggaaag tcatttcagt gttgttttgt tctgacatgt
    12841 gtagaaaacc cttaagtgtt gtccttaaat actgcgcaat gtaatgccaa cgttcacacg
    12901 ttatttcagc aggggatcca gtccaccacg tgcatccaat gcatacaagt catcacagcc
    12961 gccaatgtgc tgtgcgtcaa taaaaatctg gggaaccgtg gtgcgaccgc tgcgtttgat
    13021 catctcttca cgcttggcgg cattgccatc gatcggcaac tcctgaaaac tcacgccctt
    13081 gctgctcagc agtgcttttg cacgatggca atacgggcag gtttctttgg tatagatttc
    13141 aacattggcc ataatttagc tcctgttttt ttaccctgaa atttcatgtt gcagggcggc
    13201 tgcaaaatgg agtgtcccca gaagcttata acaataagtc gctgggggcc taattgcagc
    13261 taacggcctg catcatgaaa gacgacaggt aaattatttg ccgcgcacca gaggcaagtt
    13321 ttcgccagcc cagccagcaa cgccctcttt cagcacgaat acttgcgcaa aaccagcttt
    13381 cgtcagcgcg tttgcaggct cctggcactg catgccagaa ccgtctacca cgataaccgg
    13441 tttgtctttg tgcttctcaa gctcaccaac attgttggct ttgatttcgc tcggcaacag
    13501 attgatagaa cctgcgatat ggcctttacg gaagtcatca cgctgacgta aatccacaac
    13561 cacagcgtct tctttgttga tcagacgcgt agcttcacca cgagtaatca ccttcacttt
    13621 cgaggtcagg ctcttaaacg tagtcacaag aaccgccacc agtaacgcga tccaggcgat
    13681 actcagtatg ggatgacggc caacaaattg cataatttct tgcatggggg gtaacaactc
    13741 ccgacgtagt gattaaaaaa accaggaaag gagtatacct gcgcggtgcg gcaaatacag
    13801 ccagcgcgtt aactggaatg caattttgcg gggcacgacg aaaaaaaagc gcaaatctgg
    13861 catcggcttg ccgcgagcgg tcgtatattt tgatcttcag aggctatttt atcgattcag
    13921 ctgtagtaaa attacgcaaa ttttgactct tgagtatgag gttgtcgcaa tgtcggtttc
    13981 taaaaaacct atggtactgg tgattctgga tggctatggc tatcgtgaag aacagcagga
    14041 taacgccatt tttagtgcta aaaccccggt aatggatgca ctgtgggcca atcgtccgca
    14101 taccctgatc gacgcttccg gtctggaagt cggtctgcct gaccgtcaga tgggtaactc
    14161 cgaagtaggc cacgttaacc tgggtgccgg ccgcatcgtg tatcaggacc tgactcgtct
    14221 ggacgttgaa atcaaagatc gcgctttctt tgctaatcca gtgctgactg gcgcggtaga
    14281 taaagcgaaa aacgcaggta aagcagtaca cattatgggt ctgctctccg caggcggcgt
    14341 acacagccac gaagatcaca tcatggcgat ggtagaactg gcagctgaac gcggcgcaga
    14401 aaaaatctac ctgcacgcat tccttgacgg tcgcgacacc ccgccgcgca gtgctgaatc
    14461 ctcgctgaaa aaatttgaag agaaatttgc tgcgctgggt aaaggccgcg tagcgtccat
    14521 cattggtcgt tactacgcga tggaccgcga taaccgttgg gatcgcgttg aaaaagccta
    14581 tgacctgctg actctggcgc agggtgagtt tcaggccgat actgccgttg ctggtttgca
    14641 ggcagcttat gctcgcgacg aaaacgacga gttcgtgaaa gcgaccgtta ttcgtgcaga
    14701 aggtcagcca gatgcggcaa tggaagacgg cgatgcgctg attttcatga acttccgtgc
    14761 tgaccgcgcg cgtgaaatca ctcgtgcatt cgtgaacgct gatttcgatg gcttcgcgcg
    14821 taagaaagtg gttaacgtcg atttcgtgat gctgaccgaa tacgccgctg acatcaaaac
    14881 tgcggttgct tacccacccg cttccctggt taacaccttc ggcgagtgga tggcgaaaaa
    14941 cgacaaaact cagttgcgta tttccgaaac cgaaaaatat gcccacgtta ctttcttctt
    15001 caacggtggc gtagaagagt cgttcaaagg cgaagatcgc attctgatca actcaccgaa
    15061 agtggctacc tacgatctgc aaccggaaat gagctccgca gagctgaccg aaaaactggt
    15121 tgcggccatc aagagcggca aatacgacac cattatctgt aactatccga acggcgacat
    15181 ggtaggtcac accggggtga tggaagcggc ggttaaagcg gttgaagcac tggatcactg
    15241 cgtggaagaa gtcgcgaaag cggttgagtc cgtgggtgga caactgctga tcaccgctga
    15301 ccacggtaac gctgaacaga tgcgcgatcc ggcaacgggt caggcacaca cggcacacac
    15361 caacctgcca gttccgctga tttacgttgg tgataagaac gtgaaagcgg ttgcaggcgg
    15421 caaactttct gacatcgcgc cgaccatgtt gtcgctgatg ggtatggaaa tcccgcaaga
    15481 gatgactggt aagccgctgt tcatcgtgga ataatccctc cccatgaggg gaaaggcgat
    15541 taataccatg acacgggccg tgaaaccgcg caggtttgca atcaggccca tcatctacgc
    15601 cagcgttctt agcgctggcg tattgttgtg cgccttttcc gcccacgcgg atgagcgtga
    15661 ccaactcaaa tctattcagg ccgatatcgc cacaaaagag cgcgcggtac gccaaaagca
    15721 acaacaacgc gcaagcctgc tcgcacaatt gaaaaagcag gaagaagcga tctctgaagc
    15781 cacccgtaag ctgcgcgaaa cgcaaaacac gctcaatcaa ctcaataaac agattgatga
    15841 gatgaacgcg tcgattgcca aactggagca gcaaaaagcc gcccaggagc gcagcctcgc
    15901 cgcacaactg gatgccgcat tccgtcaggg cgagcatacc ggtattcagc taattctcag
    15961 cggtgaagaa agccagcgtg gacagcgttt acaggcttat ttcggctatc tcaaccaggc
    16021 gcgacaagaa accattgccc agttgaagca aacgcgtgaa gaagtcgcca tgcagcgtgc
    16081 tgaactggaa gagaaacaaa gcgagcaaca aacgctgtta tatgaacagc gcgcccaaca
    16141 ggcgaaactg actcaggctc tgaacgagcg taaaaagacg ctggcggggc tggaatcttc
    16201 catccagcaa ggtcagcagc agctaagtga actgcgtgcc aacgaatccc gtctgcgtaa
    16261 cagtattgcc cgtgcggaag ccgcggcgaa agcgcgtgct gaacgagaag cgcgtgaggc
    16321 tcaggcagtt cgcgacaggc agaaagaagc gacgcgcaaa ggcaccacct acaaaccgac
    16381 cgaaagcgaa aaatcgctga tgtcccgaac tggtggcctg ggtgcgccgc gtggtcaggc
    16441 attctggcca gttcgtggcc ctacgctgca tcgctatggc gagcagctac agggtgaatt
    16501 acgctggaaa ggaatggtta tcggtgcctc tgaaggtact gaagttaaag caattgccga
    16561 cggtcgggtg attctggctg actggctgca aggctacggt ctggtggtgg tggttgagca
    16621 cggtaaaggc gatatgagtc tttacggcta taatcaaagc gcgctggtga gcgttggttc
    16681 gcaggttcgt gcgggccagc caattgcact ggtgggcagc agtggcggtc agggcagacc
    16741 ttcactctat ttcgaaattc gccgccaggg tcaggccgtc aatccacagc cgtggttggg
    16801 aagataagtt ttgtttccat ttcgtcgtaa cgttcttgca tttgccgctc tgttggcgct
    16861 ctcctcccct gtaattgctg gcaaacttgc catcgtcatt gatgattttg ggtatcgccc
    16921 gaacaacgaa aaccaggtgc tggcgatgcc ttccgctatc tccgtcgctg tattacccga
    16981 ttcaccgcac gccagagaga tggcgaccaa agcgcataac agcggtcacg aagtgttgat
    17041 tcatctcccg atggcaccgt tgagtaaaca gccgctggag aaaaatacgc tgcgcccgga
    17101 gatgagcagc gacgaaattg agcgcattat tcgtagtgcg gtcaataacg tgccctatgc
    17161 cgtggggatc aataaccaca tgggcagcaa gatgacctct aacctgtttg gtatgcagaa
    17221 agtgatgcag gcgctggagc gttataatct ttacttcctc gacagcgtaa ccattggtaa
    17281 tacccaggcg atgcgcgccg cgcaaggcac tggcgtgaag gtgatcaaac ggaaggtgtt
    17341 cctcgacgat tcacagaacg aagcggacat ccgtgtgcaa ttcaatcgcg caattgacct
    17401 agcgcgtcgc aacggatcaa ccattgccat tgggcatcct cacccttcaa cggtacgcgt
    17461 gttgcaacag atggtttata acctgccgcc agacattacg ctggtgaaag ccagcagctt
    17521 gctgaatgaa ccgcaggttg atacgtctac accgccgaaa aacactgtgc ccgacacacc
    17581 gcgtaatccg ttccgtggcg tgaagctgtg caaaccgaag aaaccgatag aacctgttta
    17641 tgctaatcgc ttctttgaag tattaagcga aagcatcagc cagagcacgc tgatcgttta
    17701 cttccagcat cagtggcaag gctggggcaa acagcctgaa gcggcgaagc ttaacgctag
    17761 cgtaaattaa aggcgctacg cgtaatgcga tggtgcgacc gcgttttgtc gcgccattgc
    17821 cataagcgga aagaccacag taacgcctga taacccacct ttacgctgcg cacattggta
    17881 atcaggcgct tatacatac
//
LOCUS       DAZPLI010000078        17448 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZPLI010000078 DAZPLI010000000
VERSION     DAZPLI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.079"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..502)
                     /locus_tag="ACHUU3_002518"
     CDS             complement(<1..502)
                     /locus_tag="ACHUU3_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR2082547.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQR"
     gene            complement(688..772)
                     /locus_tag="ACHUU3_002519"
     tRNA            complement(688..772)
                     /locus_tag="ACHUU3_002519"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Leu,seq:caa)
     gene            968..1987
                     /gene="ahr"
                     /locus_tag="ACHUU3_002520"
     CDS             968..1987
                     /gene="ahr"
                     /locus_tag="ACHUU3_002520"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR2082548.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="ACHUU3_002521"
     CDS             complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="ACHUU3_002521"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR2082549.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2771..3802
                     /gene="idnD"
                     /locus_tag="ACHUU3_002522"
     CDS             2771..3802
                     /gene="idnD"
                     /locus_tag="ACHUU3_002522"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFR2082550.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3826..4590
                     /gene="idnO"
                     /locus_tag="ACHUU3_002523"
     CDS             3826..4590
                     /gene="idnO"
                     /locus_tag="ACHUU3_002523"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFR2082551.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4655..5974
                     /gene="idnT"
                     /locus_tag="ACHUU3_002524"
     CDS             4655..5974
                     /gene="idnT"
                     /locus_tag="ACHUU3_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFR2082552.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6041..7039
                     /gene="idnR"
                     /locus_tag="ACHUU3_002525"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6041..7039
                     /gene="idnR"
                     /locus_tag="ACHUU3_002525"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFR2082553.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7117..8619
                     /locus_tag="ACHUU3_002526"
     CDS             7117..8619
                     /locus_tag="ACHUU3_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR2082554.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="ACHUU3_002527"
     CDS             complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="ACHUU3_002527"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR2082555.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="ACHUU3_002528"
     CDS             complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="ACHUU3_002528"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR2082556.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(10983..11033)
                     /gene="ytgA"
                     /locus_tag="ACHUU3_002529"
     CDS             complement(10983..11033)
                     /gene="ytgA"
                     /locus_tag="ACHUU3_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR2082557.1"
                     /translation="MSIIILATISTTQENK"
     gene            11187..12698
                     /gene="pepA"
                     /locus_tag="ACHUU3_002530"
     CDS             11187..12698
                     /gene="pepA"
                     /locus_tag="ACHUU3_002530"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR2082558.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12832..13275
                     /gene="holC"
                     /locus_tag="ACHUU3_002531"
     CDS             12832..13275
                     /gene="holC"
                     /locus_tag="ACHUU3_002531"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR2082559.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13275..16130
                     /gene="valS"
                     /locus_tag="ACHUU3_002532"
     CDS             13275..16130
                     /gene="valS"
                     /locus_tag="ACHUU3_002532"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR2082560.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16186..17382)
                     /locus_tag="ACHUU3_002533"
     CDS             complement(16186..17382)
                     /locus_tag="ACHUU3_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFR2082561.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4483 a         4336 c         4454 g         4175 t
ORIGIN      
        1 tgcgctgttg aagacgagca gccacttcaa gacgtccaga catttctacg gccttaatag
       61 gtgctaataa atctcgagtt tttaactcag cgatattacg ttcaccaagc gctgcaaaaa
      121 gattatcttc cagacttttt agcacacgat gggcatgatc ttcagaccac tttttattgg
      181 tgccatgcca ctcaatcgcg acttctttaa aggttcgtgc tttactctgt tcaaccttat
      241 cacttttctt tttgtctccc ggatcgacgc cattcgcaag cagcttacgt ggctcgtcac
      301 gacgtattct ggcatccgcg agtgtgattt caggataaac cccaagtgcc agcatctttt
      361 gctttccctc ataacggtac tgcaaacgcc agtacttaga accatttgga tggacaagca
      421 gatgcatacc gccaccatca gtcaacttat attgcttatc tgttggctta gctgttctga
      481 ctttgatgtc tgttaatgcc atgcgtattc cctccctgtt ggtacaagca ttatcgaacc
      541 ggaaatacca tcatctgtac caacatatgt tggtagaagt acgttgatat cggttgacct
      601 tgagagagtt aatatagcgg gaaaggtaga taaatactga atttcaggca taaaaaagac
      661 ctcagttgag gtctattgac atacttttgg tgccgaaggc cggactcgaa ccggcacgta
      721 tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga
      781 ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg
      841 ctcgcacctc gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt
      901 cacactccag gcaccccgcc ctgccatgct ctacacttcc cagacaacac cagagaagga
      961 ccaaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt
     1021 ttatgagtac gagcccggtg aattgaagcc acaagatgtt gaagtgcagg tggattactg
     1081 cgggatctgc cattccgatc tgtcgatgat cgacaacgaa tggggatttt cacagtatcc
     1141 gctggttgcc gggcatgagg tgattgggcg cgtggtggcg ctcgggagcg ccgcgcagga
     1201 taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta gctgcgggca
     1261 ttgcgacgcc tgtattagcg gtaatcagat caactgcgag caaggtgcgg tgccaacaat
     1321 tatgaatcgc ggcggttttg ccgagaagtt gcgtgcagac tggcaatggg tgatcccact
     1381 gccggaaaat atcgatatcg agtccgccgg gccgctgctg tgtggtggta tcacggtctt
     1441 taaaccactg ttgatgcacc atatcactgc taccagccgc gttggggtaa ttggtattgg
     1501 cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacagcctt
     1561 tagttcgaat cctgcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa
     1621 tagccgcgat ccgcaggcac tgaaaacact tgcggggcag ttcgatctca ttatcaacac
     1681 cgtcaacgtc agcctcgact ggcagcctta ttttgaggcg ctgacgtatg gcggtaattt
     1741 ccacaccgtc ggtgcggtcc tcacgccgct gtctgttccg gcctttacgt taattgcggg
     1801 cgaccgcagc gtctctggtt cagcaaccgg cacaccttat gagcttcgta agctgatgcg
     1861 ctttgcggcc cgcagcaagg ttgcgccgac caccgaactg ttcccgatgt cgaaaataaa
     1921 cgacgccatc cagcatgtgc gcaacggtaa ggcgcgttac cgcgtggttc tgaaagccga
     1981 tttttgaaaa tcattcgcag cgctgatctg aggcgctacc ccctttcgca catattctgt
     2041 cttgtcgtat cgccagcacg gcctgccgac attgttcggt gacattggca atatcatggt
     2101 tgatatcaat gcgtacaata tcctgttcat ctgcttgtgg acgctccagc gcctcaaact
     2161 gactttttag taacgctacc ggcataaaat gcccagcccg ccgctgcatt cgcgcgagaa
     2221 tagtttcata gtcgccatct aaccagagga aatgaacatg ggggctaccc ttgcgtaaaa
     2281 tatcacgata ctgttttttt aatgatgaac agacaataaa tcctgtttca ttctttttat
     2341 aaagactgta tgaagcatca tttaagcgtt ccagccaggg aagtcgatct tcatcagata
     2401 atggaatacc ctgcgacatt ttatctatat ttttggctgg atgaagatcg tcaccatcaa
     2461 taaatttagc agataataac gcggcaacct tgctaccaat taatgtttta ccactccctg
     2521 aaacgcccat caaaataaag ctttcacccg ccatttaaaa tctcctgaat catcttacct
     2581 gctgcgatac tacgcctggt ggcataataa tttcacgttt ttttatttct gctgataaga
     2641 attacaaggc acatcacgtt atgcgtaaca tagtaatgta acaattttct gacgtgatct
     2701 tcatcacaaa taatgacaat taaaaccgct taaatgcttc cgaggtgtct acctgaccag
     2761 tgaggaattt atgcaagtga aaacacagtc ctgcgttgtt gcggacaaga aaactgttgc
     2821 cgttaccgag cagacgatag attggaataa taatggaaca ttagtacaaa taacccgagg
     2881 tggaatttgc ggttccgatt tacattatta tcaggaagga aaagtaggta atttcatgat
     2941 aaaggcaccg atggtgttag gtcatgaagt tatcggtaaa gttattcata gcgactcatc
     3001 agaattacat gaagggcaaa cggtagccat taatccgtct aaaccgtgcg gtcactgcaa
     3061 atactgcatt gaacataacg agaatcagtg tacagatatg cgtttttttg gcagtgccat
     3121 gtatttccct catgttgatg gtggttttac ccgttataaa atggtcgaaa cgtcgcaatg
     3181 tgtcccttat ccggccaaag ctgacgaaaa ggttatggct tttgccgaac ctttagccgt
     3241 cgcgattcat gccgcacatc aggccgacga gttacagggc aagcgagtat ttgtttccgg
     3301 tgttgggccc attggctgcc tgattgtcag tgctgtgaaa acactggggt ccgcggaaat
     3361 tgtctgtgcc gatgtgagtc cccgttccct ttcgctggcc aaagagatgg gtgcggatgt
     3421 gctcgtaaac ccacaaaacg acgacatgga tcactggaaa gcggaaaaag gccatttcga
     3481 tgtcagcttt gaagtgtccg gtcatccttc atcagtgaat acctgtctgg aggtcactcg
     3541 tgcacgcggc gtaatggtgc aggtagggat gggaggcgcg atggcagaat tcccaatgat
     3601 gacgttgatt ggtaaggaga tttcactcaa aggctctttc cgttttacca gcgaatttaa
     3661 taccgcagtg tcatggctgg cgaatggcgt tatcaatcca ctgcctttac tgagtgctga
     3721 atatctcttc actgacctgg aagaggcgct acgtttcgcc ggtgataaaa cccaggcagc
     3781 aaaagtccag cttgttttct aagagataaa taaaggaata atacaatgaa cgatctattt
     3841 tcactggcag gaaaaaatat cttgattacc ggttcagcac agggcattgg ctatttactg
     3901 gcaaccggcc tgggtaaata tggcgcacaa ataattatta atgatattac tgccgaacgc
     3961 gcagaacttg ctgtagaaaa actccaccag gagggtattc aggccgttgc cgcacctttt
     4021 aatgttactc ataaacatga aattgatgcc gccgttgaac atatcgaaaa ggacatcggc
     4081 cccattgatg tgctggtgaa taacgccggt atccagcgcc gtcatccttt tactgagttc
     4141 cctgaacaag agtggaatga tgtgatcgca gtaaaccaga ccgccgtgtt cctggtatcg
     4201 caagcggtaa ctcgtcacat ggttgaacgc aaggcaggta aagttattaa tatttgctcg
     4261 atgcaaagcg aactgggacg tgacaccatc accccttatg ccgcatcgaa aggggcggta
     4321 aaaatgctca cccgcggcat gtgcgtcgag ctggcgcgcc acaatattca ggtcaacggt
     4381 attgcgccgg gctatttcaa aacagaaatg actaaagcac tggttgagga cgaagccttc
     4441 accgcctggt tgtgcaaacg gacccccgcc gcacgctggg gagatccgca ggaactgatt
     4501 ggtgctgcgg tgttcctttc ttcaaaagcc tctgatttcg taaacggcca cctgttgttt
     4561 gttgatggcg gcatgttagt ggctgtttaa cagccaccac cctacgtgca gcgcgaaagc
     4621 gctgcttttc tggcactaat aagagataaa gactatgcca ttaatcatta ttgcggcagg
     4681 cgtcgcgctg cttcttatcc tgatgatcgg ctttaaagtt aacggcttta ttgccctcgt
     4741 tctggtagct gccgtcgtcg gatttgccga agggatggat gcacaggccg tcctgcactc
     4801 tatacaaaat ggtatcggca gcacgctcgg cgggctggca atgatcctcg gtttcggggc
     4861 catgttaggc aagctgattt ctgatacggg cgcggcacaa cgtatcgcca ctacgctgat
     4921 tgctactttt ggtaaaaaac gcgtgcaatg ggcgctagtg atcaccggtc tggttgtggg
     4981 cctcgccatg ttttttgaag tgggttttgt cctgctgttg ccgttggtat ttaccatcgt
     5041 ggcatcatct ggattacccc tgttgtatgt tggcgtaccg atggtagcag cgctctctgt
     5101 aacccactgt tttctgccgc cacatccagg gcctactgcc atcgcgacta tctttgaggc
     5161 taatctcgga acgactttac tgtatggatt tatcattacc attccgacag ttattgtcgc
     5221 aggaccgctg ttttctaaac tgctaactcg ctttgagaaa gcaccaccgg aaggcttatt
     5281 taatcctcat ctgtttagcg aagaggagat gccctccttc tggaacagta tttttgctgc
     5341 agtgatcccg gtcatcctga tggctatcgc cgccgtttgt gaaattacgt taccgaaaac
     5401 taacaccgtg cgcctcttct ttgaatttgt cggtaaccct gccgttgcgc tgtttattgc
     5461 cattgttatt gcgattttca cactgggccg acgtaatgga cgcaccatcg agcaaatcat
     5521 ggatatcatt ggggattcta taggcgctat cgcgatgatt gtgtttatta tcgctggcgg
     5581 cggcgcgttt aagcaggtat tagtagatag tggtgtcggg cagtatattt cacacttaat
     5641 gaccggaact acgctttcgc cgttattgat gtgctggact gtcgcggcgc tgttgcgtat
     5701 cgctctgggc tctgccaccg tcgcggccat taccaccgcg ggtgtggtgt tgccgattat
     5761 caacgttacc catgccgatc ccgctttaat ggtactggca actggtgcgg gcagcgtgat
     5821 cgcgtcacac gtaaacgacc ctggcttctg gctatttaaa gggtatttta atctgacggt
     5881 tggtgaaacg ttgcgtacct ggacagtgat ggaaaccctt atttctatta tgggtttgct
     5941 gggcgtgtta gccattaacg ccgtattgca ctgaaagaat aaaatattgt gatgtagttt
     6001 cgggggaaga ttgaagattc ccccgacgtt gggagaagtt atgcgcaatc acagaatttc
     6061 tttacaggat atcgctacgc tggctggcgt aacaaaaatg accgtgagtc gttatatccg
     6121 ctcgccgaaa aaggtggcaa aggaaacagg cgagcgcatc gccaaaatta tggaagagat
     6181 caattatatc cctaatcgcg ctcccggcat gttgttgaat gcacaaagtt ataccctcgg
     6241 tatcttgatt ccgtcatttc aaaatcaact cttcgctgac attctcgcgg gaatagaatc
     6301 cgtcacctct gtgcataatt atcagaccct tattgctaat tacaattacg atcgcgattc
     6361 cgaagaagag tcggtgatta acctgctctc ttataacatt gacgggatta ttctttcgga
     6421 aaaatatcac accatcagga cggtgaaatt tctgcgttca gccaccattc cggtcgtgga
     6481 attgatggat gtacagggag aacggctgga tatggaggtc ggttttgata atcggcaggc
     6541 tgcttttgac atggtgtgta ccatgctgga taagcgagtc aggcgcaaaa ttctgtatct
     6601 cggttccaaa gatgacaccc gcgatgaaca gcgttatcag gggtattgcg atgcgatgat
     6661 gctgcataat ctttccccat tacgcatgaa tccacgcgcc atctcatcca ttcatttagg
     6721 aatgcagtta atgcgtgatg cccttagcgc aaacccggat ttagacggtg tattttgcac
     6781 caatgacgat attgcgatgg gtgcgcttct gctgtgccgc gagcgaaacc tcgccgtgcc
     6841 agaacaaatt tccattgcgg gttttcatgg gctggagatt ggcaggcaaa tgatcccaag
     6901 cctcgccagc gtaattactc cccgttttga tattggtcgg atggccgccc aaatgttgct
     6961 gagcaagatt aagaataacg atcacaacca caacactgtt gacctcgggt atcagattta
     7021 tcacggtaac acgctttagg ttattttctc ttcattacac atattctctt tgccggacgt
     7081 tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct gttaattgcc
     7141 cgcacgccgg acacagaact gtttttactg ccgggaatgg ctaaccgtca cgggctgatt
     7201 actggcgcaa cggggacggg taaaactgtc acgttgcaaa aactggcaga gtcattgtcg
     7261 gaaatcggtg ttccggtgtt tatggccgat gtgaaaggcg atctgaccgg catcgcgcag
     7321 gcaggaacgg cgtcggaaaa actgctcgca agactgaaaa atatcggcgt caacgactgg
     7381 caaccgcatg ccaatccggt ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg
     7441 cgggcgacgg tttcggatct ggggccgctg ttgctggcac gactgttgaa tctcaacgat
     7501 gtgcaatctg gcgtgctgaa tattatcttc cgcattgcgg acgatcaggg attgttgctg
     7561 ctcgacttta aagatttgcg ggcgattacc cagtacatcg gcgataacgc caaatccttc
     7621 cagaatcagt acggaaatat cagtagcgca tcggttggtg ccatccagcg cggattactg
     7681 tcgctggaac agcaaggcgc agaacacttc tttggtgagc cgatgctgga tatcaaagac
     7741 tggatgcgca ccgatgccaa cggtaaaggc gttatcaata tcctcagcgc cgagaaactt
     7801 tatcagatgc cgaaactgta cgccgccagc ctgctgtgga tgctttcaga gttatatgaa
     7861 caattgcctg aagcgggcga tctggagaaa ccaaaactgg tgtttttctt cgacgaagca
     7921 catctgctgt ttaacgatgc gccacaggta ctgctggata agattgagca ggtgataagg
     7981 cttattcgct caaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc agatatcccg
     8041 gataacgtgc tagggcagct cggtaatcgc gttcaacacg ctttgcgtgc ttttacgcct
     8101 aaagatcaga aagcagtgaa ggcagcggcg caaaccatgc gggccaatcc ggcgtttgat
     8161 accgaaaagg caatccagga actggggacc ggcgaggcgt taatctcgtt tctcgatgcg
     8221 aaaggaagtc cttcagtggt ggaacgggcg atggtgatcg caccttgttc gcgaatgggg
     8281 ccggtgacgg aagatgagcg taatggcctg attaatcact ccccggtgta tggcaaatat
     8341 gaagatgagg tggaccgcga gtccgcctat gagatgctgc aaaaaggctt tcaggccagt
     8401 accgagcagc aaaataatcc ccccgtgaaa gggaaagagg tggcggtgga tgacggtatt
     8461 cttggtggat tgaaggatat tttgtttggc actaccggac cacgcggcgg gaagaaagat
     8521 ggtgtggtgc aaacaatggc gaaaagcgcc gctcgccagg tgacgaatca gattgtacgc
     8581 gggatgttgg ggagtttgct gggggggaga aaaaggtaag tcagacgtga tctgtgcccc
     8641 tcaccctaac cctctcccca aaggggcgag gggaccgttc gaactcgtat ttgactttgt
     8701 caacaataat aaagggagct ttcgctccct tgttagatta cgattttctc attaacagcc
     8761 acaggctgat taagaagaag ctggcgcttg gcaacagtgc gccgatgatc ggcgggatgc
     8821 cataaaccaa cgtcagcggg ccgaagatct ggtccagtac gtagaagaca aaaccgaagc
     8881 tgataccggt gaccacacgc acgcccatcg gtacgctacg cagtgggcca aagatgaacg
     8941 acagcgccat caacatcatc accgccacgg atagcggctg gaagattttg ctccacatgt
     9001 tgagctgata acgtccggca tcctgaccgc tcgacttcag atacttcaca tagttgtgca
     9061 aaccactgat ggagagtgca tccgggtcca gcgccaccac gcccagtttg tctggcgtga
     9121 ggttggtttt ccaggtgccg ctcaccgtct gcgaaccggt gatctgtttc ggattggtca
     9181 gatcagattc atcaacctgc gacagacgcc agactttatg ttccgggtca aacttcgcgg
     9241 tagcggcata acgtacggat tgcagacgac gattctcgtt aaaggcataa atactgatgc
     9301 cacctaactc ttcgtcacct ttaacccgct caatgtagac gaagttgttg ccatctttcg
     9361 cccataagcc ttgctgagtg gagagcaacg agccgccgta catcgcctgc gcacggtagt
     9421 tacgcgccat ctgctcgccc tgcgacgcga cccattcacc aatcgccatc gtcagcaaga
     9481 ccagcggaat ggcggttttc atcaccgaca gcgccacctg catacgggta aaaccagaag
     9541 cctgcatcac caccagttcg ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa
     9601 gcagagccgc catcgggaag aagatttgca catctttcgg cacgctcagc aaggtataca
     9661 ttcctgcgcc taacgcgtcg taactcccct gcccggcttt tttcagctga tcgacaaact
     9721 tgataatgcc cgacagcgac accagcatga acagcgtcat catgatggtg gtgaaaatag
     9781 ttttaccgat atagcggtca agtacgccaa aaggttgcat cacaccgctc ctttacgcga
     9841 aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa aggttgagaa caatcgccaa
     9901 agccagataa atcaggttaa cggtccacat ccacagcgtc gggtccagct tacctttacc
     9961 gccgttcgat ttcagggagg tctggatcag gaagaaaagt agatacagca gcatggctgg
    10021 cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg ctcaacggta caaccataag
    10081 tgccatcata aacacggtga ataccaacgt gatacgccag ttcagttctg cgcgggcacg
    10141 atcggtgtca gtgttccaca atgtgcgcat gtccatctgg tcggtatcgt tcgggtcgag
    10201 caccaccgcc tggtgaccaa tgatcgcctg ataatcctga aagtccgtaa tgcggaaatc
    10261 gcgtaacagt gcagtgcctt cgaagcgcgt tccctggttg agagtgacga cctgggagcc
    10321 gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc acagaaggac gtgcattacc
    10381 ttttggtcga atttgtgcga ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat
    10441 gaacagcacc gagctgccat tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat
    10501 gccagggttc gctttcgctt ctgctaacac ttcatcctga tgacgcgatg accatggtcc
    10561 cgcccacatc acgttaaccg ccgcgacgat tgccgtgaac accgcaagga tcattgccgc
    10621 tttcaccagc accgctttgc tcagaccgca ggcatgcatt accgtaattt cactttcggt
    10681 atacagtttg cccagcgtca tcagcagccc gaggaacagg cttaatggca ggataagctg
    10741 cgccatttcc ggcacgccca acccgagaag ggagagcacc agattcgccg gaatatcgcc
    10801 gtcaaccgct gcgccgagga tcctcactaa cttttgacag aagaagatca aaagcaagat
    10861 gaagagtatc gccagctggc ttttgagcgt ctcccgcacc agatatctta tgattatcac
    10921 tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt agcttgtttc atggcttaaa
    10981 cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga taattatact caacggattc
    11041 acctctcaga ttttgttctg acgtgccaat gccgtaataa cgttaagatt aacacgaagt
    11101 catcgcaaca gcggacatga gttacgaaag cttgcaattc tatctgtagc caccgccgtt
    11161 gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt aaaaagcggt agcccggaga
    11221 aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg
    11281 cagaacagct cgataaaatc agcgatgggt acatcagcgc cctgctacgt cggggcgaac
    11341 tggaaggaaa accggggcag acactgttgc tgcaccatgt tccgaatgta ctttccgagc
    11401 gaattctcct tattggttgc ggcaaagaac gtgagctgga tgaacgtcag tacaagcagg
    11461 ttattcagaa aaccattaat acgctgaatg atactggctc aatggaagcg gtctgctttc
    11521 tgactgaact gcacgttaaa ggccgtaaca actactggaa agtgcgtcag gctgtcgaga
    11581 ctgcaaaaga gacgctctac agtttcgatc agctgaaaac gaacaagagc gaaccgcgtc
    11641 gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc
    11701 gcgcgatcca gcacggtctg gcgattgccg ccgggattaa agcagcaaaa gatctcggca
    11761 atatgccgcc gaatatctgt aacgccgctt acctcgcttc acaagcgcgc cagctggctg
    11821 acagctacag caagaatgtt atcacccgcg ttatcggcga acagcagatg aaagagctgg
    11881 ggatgcactc ttatctggcg gtcggtcagg gttcgcaaaa cgaatcgctg atgtcggtga
    11941 ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt
    12001 taaccttcga ctccggcggt atctccatca agccttcaga aggcatggat gagatgaagt
    12061 acgatatgtg cggtgcggca gcggtttacg gcgtgatgcg gatggtcgca gagctacaac
    12121 tgcctattaa cgttattggc gtgctggcag gctgtgaaaa catgcctggc gggcgtgcct
    12181 atcgtccggg cgatgtgtta accaccatgt ccggtcaaac cgttgaagtg ctgaacaccg
    12241 atgccgaagg ccgcctggta ctgtgcgacg tgttaactta cgttgaacgt tttgagccgg
    12301 aagcggtgat tgatgtggcg acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcata
    12361 ttactggtct gatggcgaac cataatccgc tggcccatga actgattgcc gcgtctgaac
    12421 aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga gtatcaggaa caactggagt
    12481 ccaattttgc cgatatggcg aacattggcg gtcgtcctgg tggggcgatt accgcaggtt
    12541 gcttcctgtc acgctttacc cgtaagtaca actgggcgca cctggatatt gcaggaaccg
    12601 cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt
    12661 tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt ttgcgtttta ccctcaccct
    12721 aaccctctcc ctaagggaga gggaaccgat cgagcgcaat agaaattcat tgctcatctt
    12781 tcagggtgag gaatgcagca atatttaaat ccaccacaag aagccccata tatgaaaaac
    12841 gcaacgttct accttctgga caatgacacc accgtcgatg gcttaagcgc cgttgagcaa
    12901 ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta agcgcgtgct catcgcctgt
    12961 gaagatgaaa agcaggctta ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc
    13021 tttgtcccgc ataatttagc gggagaagga ccgcgcggcg gtgcgccggt ggagatcgcc
    13081 tggccgcaaa aacgcagcag cagcccgcgc gatatattga ttagtctgcg aacaagcttt
    13141 gcagattttg ccactgcttt cacagaagtg gtagacttcg ttccttatga agattctctg
    13201 aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg
    13261 gcaacctgga aataatggaa aagacatata acccacaaga tatcgaacag ccgctttacg
    13321 agcactggga aaagcagggc tactttaagc ctaatggcga tgaaagccag gaaagtttct
    13381 gcatcatgat cccgccgccg aacgtcaccg gcagtttgca tatgggtcac gccttccagc
    13441 aaaccatcat ggataccatg atccgctatc agcgcatgca gggcaaaaac accctatggc
    13501 aggtcggtac tgaccacgcc gggatcgcta cccagatggt cgttgagcgc aagattgccg
    13561 cagaagaagg taaaacccgt cacgactacg gccgcgaagc tttcatcgac aaaatctggg
    13621 aatggaaagc ggagtccggc ggcaccatta cccgtcagat gcgccgtctc ggcaactctg
    13681 tcgactggga gcgtgaacgc ttcacgatgg acgaaggcct gtccaatgcg gtgaaagaag
    13741 ttttcgttcg tctgtataaa gaagacctga tttaccgtgg caaacgcctg gtaaactggg
    13801 atccgaaact gcgcaccgct atctctgacc tggaagtgga aaaccgcgaa tcgaaaggtt
    13861 cgatgtggca catccgctat ccgctggctg acggcgcgaa aaccgcagac ggtaaagatt
    13921 atctggtggt tgcgactacc cgtccggaaa ccctgttggg cgataccggc gtggccgtta
    13981 acccggaaga tccgcgttac aaagacctga ttggcaaata tgtcattcta ccgctggtta
    14041 accgtcgtat tccgatcgtt ggcgacgaac acgccgacat ggaaaaaggc accggctgcg
    14101 tgaaaatcac tccggcgcac gactttaacg actatgaagt gggtaaacgt cacgcgctgc
    14161 cgatgatcaa catcctgacc tttgacggcg atatccgtga aagcgcccag gtgttcgata
    14221 ccaaaggtaa cgaatctgac gtttattcca gcgaaatccc ggcagagttc cagaaactcg
    14281 agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga cgcactcggc ctgctggaag
    14341 aaattaaacc gcacgacctg accgttcctt acggcgaccg tggcggcgta gttatcgaac
    14401 caatgctgac cgaccagtgg tacgtgcgtg ccgatgtact ggcgaaaccg gcggttgaag
    14461 cggttgagaa cggcgacatt cagttcgtac cgaagcagta cgaaaacatg tacttctcct
    14521 ggatgcgcga tattcaggac tggtgtatct ctcgtcagct gtggtggggt caccgtatcc
    14581 ctgcatggta cgacgaagca ggtaacgttt atgttggccg caacgaagaa gaagtgcgca
    14641 aggaaaataa cctcggtgcc gacgttgccc tgcgtcagga cgaagacgtt ctcgacacct
    14701 ggttctcttc tgcgctgtgg accttctcta ccctaggctg gccggaaaac actgacgcac
    14761 tgcgccagtt ccacccaacc agcgtgatgg tatccggctt cgacatcatt ttcttctgga
    14821 ttgcccgcat gatcatgatg accatgcact tcatcaaaga tgaaaatggc aaaccgcagg
    14881 tgccgttcca caccgtttac atgaccggcc tgattcgtga cgacgaaggc cagaagatgt
    14941 ccaaatccaa gggtaacgtt attgacccgc tggatatggt tgacggtatt tcgctgccgg
    15001 aactgctgga gaaacgtacc ggcaatatga tgcagccgca gctggcggac aaaatccgta
    15061 agcgcaccga gaagcagttc ccgaacggta ttgagccgca cggcaccgat gccctgcgct
    15121 tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa ctgggatatg aagcgcctgg
    15181 aaggttaccg taacttctgt aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca
    15241 cggaaggtca ggattgcggc ttcaacggtg gtgaaatgac gctgtcgctg gcggaccgct
    15301 ggattctggc agagttcaac cagaccatca aagcgtaccg cgaagcgctg gacagcttcc
    15361 gcttcgatat cgctgcaggc attctgtatg agttcacctg gaaccagttc tgtgactggt
    15421 atctggagct gactaagccg gtaatgaacg gtggcaccga agccgaactg cgcggtactc
    15481 gccatacgct ggtgaccgta ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt
    15541 tcatcaccga aaccatctgg cagcgtgtga aagtactttg cggtatcact gccgacacca
    15601 tcatgctgca gccgttcccg cagtacgatg catctcaggt tgatgaagcc gcactggccg
    15661 acaccgaatg gctgaaacag gcgatcgttg cggtacgtaa catccgtgca gaaatgaata
    15721 tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg cagcgcggat gcagaacgtc
    15781 gcgtaaatga aaaccgtggc ttcctgcaaa ccctggcgcg tctggaaagc atcaccgtgc
    15841 tgcctgccga tgacaaaggt ccggtttccg ttacgaagat catcgacggc gcagagctgc
    15901 tgatcccgat ggctggcctc atcaacaaag aagatgagct ggcgcgtctg gcgaaagaag
    15961 tggcgaagat cgaaggcgaa atcagccgta tcgagaacaa gctggcgaat gaaggctttg
    16021 tcgcccgcgc accggaagcg gtcatcgcga aagagcgtga gaagctggaa ggctatgcgg
    16081 aagcgaaagc gaaactgatt gaacagcagg ctgttatcgc cgcgctgtaa tcaaaacaca
    16141 gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa
    16201 agaaggcata attccccgtg aaatactggc taagaatcct ttatctggtt gttcttcttt
    16261 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    16321 aatcatccat attttcagca gtggataagc cagaccgccc gtaatcccgg atatcaccac
    16381 caacgcacac atgcgataaa gcataccgtg gtatgttaaa gttgagcgaa aacgaatacg
    16441 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    16501 ggtgctaaca gcaatcccaa aataatagat taactgcgcg attatcattt tacgttgcat
    16561 ttccataaat tgctgtaaat tctcaatatc atcatttgca tacccagaac tatcatactc
    16621 atttaatatt tgatcgaaga taatataacc agcaacagcg agaaaaggta ataaagccaa
    16681 aaatgcgaga atggcatatt ttatacaata tgtagtatct aatttcacct tgaaacgatg
    16741 tataccgaaa ctggtattac tccagagaaa actcattacc agactatata aagtgccatt
    16801 aaaaatacca atgaaaacaa tacaaactat tgtcatcaga accacagata taataacgct
    16861 acttgaacta ttggcatgta gcatctttgt cgagataaag aaaacagtcc ccatcccaag
    16921 ggccattaaa atcggcaaga aaaaggtcac ccaccagaac cctttcatag agcaattaaa
    16981 actaaatctt acgccgttga gactggtcat caaggcctga taacgcagtc cttttgctgc
    17041 cataaatata agcaaaacca acagtgacaa agtcaaaaca cagccaataa gcggcatatc
    17101 tgctgacact gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac aactaacaaa
    17161 aacattccca ccggtaatcc cataagaaaa tcgttgtccg ttaacttcca tattagcata
    17221 gagataacgc ttacatttca ttaatgccca tggtaaatag atacctagcg ttataatcgt
    17281 taacaacaca ttcaccacac aaataagaaa atatctctca cctgtaccat gaaaaacaaa
    17341 cgaatgggac ggaacatcca tatcattaat aacttgagcc ataaaagttc cctgaattaa
    17401 aaaatccatt gatataattt atatatacag acagtaaaat gttctgcc
//
LOCUS       DAZPLI010000079        17338 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZPLI010000079 DAZPLI010000000
VERSION     DAZPLI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17338
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.080"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            92..1132
                     /locus_tag="ACHUU3_002534"
     CDS             92..1132
                     /locus_tag="ACHUU3_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR2082562.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHUU3_002535"
     CDS             complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHUU3_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR2082563.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHUU3_002536"
     CDS             complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHUU3_002536"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR2082564.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5095..6126
                     /gene="galR"
                     /locus_tag="ACHUU3_002537"
     CDS             5095..6126
                     /gene="galR"
                     /locus_tag="ACHUU3_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR2082565.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHUU3_002538"
     CDS             complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHUU3_002538"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR2082566.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7517..8452
                     /gene="lysR"
                     /locus_tag="ACHUU3_002539"
     CDS             7517..8452
                     /gene="lysR"
                     /locus_tag="ACHUU3_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR2082567.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8439..9131)
                     /gene="ygeA"
                     /locus_tag="ACHUU3_002540"
     CDS             complement(8439..9131)
                     /gene="ygeA"
                     /locus_tag="ACHUU3_002540"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFR2082568.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(9262..10680)
                     /gene="araE"
                     /locus_tag="ACHUU3_002541"
     CDS             complement(9262..10680)
                     /gene="araE"
                     /locus_tag="ACHUU3_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR2082569.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10995..11756)
                     /gene="kduD"
                     /locus_tag="ACHUU3_002542"
     CDS             complement(10995..11756)
                     /gene="kduD"
                     /locus_tag="ACHUU3_002542"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR2082570.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11821..12657)
                     /gene="kduI"
                     /locus_tag="ACHUU3_002543"
     CDS             complement(11821..12657)
                     /gene="kduI"
                     /locus_tag="ACHUU3_002543"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR2082571.1"
                     /translation="MDVRQGIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12944..14125)
                     /locus_tag="ACHUU3_002544"
     CDS             complement(12944..14125)
                     /locus_tag="ACHUU3_002544"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR2082572.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14380..15609
                     /locus_tag="ACHUU3_002545"
     CDS             14380..15609
                     /locus_tag="ACHUU3_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR2082573.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            16069..16701
                     /locus_tag="ACHUU3_002546"
     CDS             16069..16701
                     /locus_tag="ACHUU3_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR2082574.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            17035..>17338
                     /locus_tag="ACHUU3_002547"
     CDS             17035..>17338
                     /locus_tag="ACHUU3_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR2082575.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVTQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT         4595 a         4523 c         3989 g         4231 t
ORIGIN      
        1 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
       61 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
      121 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
      181 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
      241 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
      301 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
      361 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
      421 ccgcgaagcg ctgcatgaca gcctcaagcg cctacacact gattacctcg atctttatca
      481 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
      541 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
      601 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
      661 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
      721 gttaaaccgc agttttgaag taggtctggc agaagtaagc cagtatgaag gggtcgaact
      781 gctggcctat tcgtgcctgg gtttcggcac gctgaccggg aaatatctca atggtgcaaa
      841 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
      901 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctag accctgctca
      961 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     1021 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     1081 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     1141 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     1201 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
     1261 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     1321 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     1381 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     1441 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     1501 atacagcgtg acacggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     1561 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     1621 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     1681 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     1741 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     1801 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     1861 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     1921 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     1981 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     2041 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     2101 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     2161 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     2221 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     2281 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     2341 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     2401 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     2461 agcaagctcc ggcacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     2521 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     2581 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     2641 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     2701 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     2761 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     2821 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     2881 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     2941 gttgatagaa acgacaggcg cgcattcggt cacgccgtag ccttcaagga tgcgcaggcc
     3001 aaatttatcc tgccaaagct gtttggtact ttcttgtaat ttttctgcgc ctgccaccac
     3061 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     3121 cgaggtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
     3181 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
     3241 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
     3301 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     3361 gacgcctttc ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     3421 ctgctgtttt acctgtgcca gacgtggcat cagcagatgc gcgaagatcc atactttgtc
     3481 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     3541 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     3601 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
     3661 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     3721 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     3781 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     3841 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
     3901 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt cgccagcgat
     3961 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
     4021 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     4081 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     4141 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
     4201 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
     4261 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     4321 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     4381 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     4441 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     4501 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     4561 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     4621 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     4681 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     4741 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
     4801 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     4861 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagca
     4921 gtcaatcgca acagcatttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     4981 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
     5041 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
     5101 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     5161 aataattcac ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtct
     5221 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     5281 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     5341 caggtggctt atcacaccgg taatttttta ttgattggca atggttacca caacgaacaa
     5401 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     5461 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     5521 ctgatcaacc gtatcctgcc tggcttcgaa aaccgttgta ttgctctgga cgatcgttac
     5581 ggtgcctggc tggctacgcg tcatttaatt caacaaggtc atacccgcat tggttatctg
     5641 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     5701 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggtgaacc agacgaaagc
     5761 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     5821 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     5881 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
     5941 ccgcgcctga ccaccgtgcg ttacccaatc gtgacaatgg cgacccaggc tgccgaactg
     6001 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
     6061 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     6121 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     6181 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     6241 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     6301 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     6361 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     6421 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     6481 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     6541 ccgcacctga gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc
     6601 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
     6661 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
     6721 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc
     6781 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     6841 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     6901 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     6961 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     7021 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     7081 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     7141 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     7201 cattaatcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     7261 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     7321 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     7381 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     7441 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatata gattaccgga
     7501 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     7561 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
     7621 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
     7681 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
     7741 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
     7801 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
     7861 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
     7921 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
     7981 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
     8041 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
     8101 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
     8161 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
     8221 ggcgggggta ggtgtttcgg tggttaaccc actcaccgca ctggattatg cggcaagcgg
     8281 tttagtggta cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
     8341 gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc
     8401 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
     8461 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga
     8521 accaataaac caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg
     8581 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
     8641 tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg cttccggaat aagacaattg
     8701 atggaaaatt gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
     8761 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca
     8821 atgtgtaaga aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta
     8881 ttggtacata acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc
     8941 agaatgtccc cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa
     9001 tcgacgctat gtagcagcac ttgcgcagag tgaagcccac caagccgctg tttaatgcct
     9061 tcatttatca gacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca
     9121 attgttttca ttcctctctc tcctgatgtg tgttaccgca acaaggctga ataaggaacg
     9181 agacaaacgc ctcaacggcc aggtgcccaa tctctattaa cgaaaaaagg gccggatgta
     9241 cagcacatcc ggcccgtgaa atcagacgcc gatatttctc aacttctcgc ctgccatcag
     9301 tttgcgttcg atatgttcca gcgtgacatt tttggtttcc ggaatgagcc agaaagtgat
     9361 gcccacaaac gcaatgttca gcgcagtgta gagccagaac gtaccggcag caccaatgct
     9421 gtcgagcagc gtcaggaagg tcgcgccgat aatcatattc gacacccagt tcgtggtggt
     9481 cgaacaggta ataccgaaat cgcggcattt cagcggctga atttcagagc acaggatcca
     9541 caccactggc gcggcgctca tcgcataacc ggcaatacac atcatcgtca tgccaacaga
     9601 gagccaggac aagccactgg aagccgtacc gttatcaaac tgcatcaggc aatagcccag
     9661 caccagagtg cctaacgcca tcacgctgaa accaattttc agagccggtt tacgccctgc
     9721 tttatctacc gtaaacaccg caataaaggt ggcgaacata aaggtcagcc ctacgaccag
     9781 agtcgcaatc atctgttgtt ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc
     9841 gtagtacatg atgatgttca taccggtaaa ctgctgcatc gcctgcaaca acataccgag
     9901 aaacacagca cgacggacgt tacggttgat cttaaacagt gcccaaccgc cctgttttaa
     9961 cttcaggctt tcacgaattt cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag
    10021 catacgcaac acttcttccg cctcaatatg acgccccttt tccgccagcc agcgcgggct
    10081 atttggcagg aagaccacca gaataatcag caggactgct ggtaaagcaa gaacccccaa
    10141 cattgcgcgc cagttaccgc tataactgaa cgctgtatcg gataaaaacg ccagcacgat
    10201 gccgagtgtg accatcaact ggtacatact gatcatctta ccgcgaacgt tttcacttgc
    10261 catttcagaa agatacagag gagcggtgta agacgcgatc ccgacagcaa tgcccagcac
    10321 cacccgagcg gcgattaaca tctctacgct ggtcgcaaaa gcggacccta tagaaccgag
    10381 tacaaacagg atggcccccg ccatcaggct gtatttacgc cccaggcgga acgacagcca
    10441 accattaaac agcgcaccaa ttgctgcgcc gagcatcatg ctactaacca cccattcctg
    10501 caaacgactg gtcagcacaa agtgatcggt aatgaacggc aacgctccgg cgattacgcc
    10561 gatatcaaga ccaaataaca atcctgcgac cgcagcagct accgaaacaa acatattcat
    10621 acgccgcgta tcccgcaaag aacgtggcgt taaagcagat tccgtattga tagtaaccat
    10681 tttttcctgc cagcagagag taagacatag tgaaaaaata cgtgaacaac tcacacaggt
    10741 gtcaggtcgg aaacagcata aatatggatt aaattgctgc gacatgtcgt tatgtgatgg
    10801 atattccaat tttcaaatta agttgaatta ttgagattat tattaaccac ctaattttac
    10861 agcagataaa attcataaag ttcattaatt gataattgat atggattatt tcataaccat
    10921 gatatgaatt atgatgacct acaggtataa aaaaccctgc catgaggcag ggtcataaaa
    10981 gtaagaggaa tgaattaacg agccagccaa ccgccatcta cggcaatggt ataaccattc
    11041 acataatctg aagcgctgga ggcaaggaac actatcggcc ccatcaggtc actcggcagt
    11101 ccccaacgac cagctggaat gcggtcgaga atttccgcgc tacgttgttc atctgcccgt
    11161 agttgttgag tattgttggt cgccatgtaa cccggagcta tcgcattaac attaatgttg
    11221 tgtttagccc attcgttcgc catcaatcgc gtcacgccca tcacgccgct ttttgatgcg
    11281 gtataagaag gcacacggat cccgccctgg aaggagagca ttgacgtgat attgataatc
    11341 ttgccgccat tgcgttgcgc gataaagtgt ttcgccgctg cctgagacat gaagaatacg
    11401 ctcttgatat tcaggttcat gacatcgtcc cagtcttttt cgctgaactc gagagcatct
    11461 tcgcggcgaa tcaatccggc gttattcacc aggatatcaa tatgaccaaa ctcagctacc
    11521 gcgcgatcca gcagtgctgg aatgccatca atctttcgca gatcggcggt caggcttaaa
    11581 aaacgacgcc ccagcgccgt gacctgcttg atggtttcag tcggttcaac gatgttaatg
    11641 ccaacaatgt cacagcccgc ttgcgccagc cccaacgcca tcccctggcc cagtccagta
    11701 tcacaaccag tgacgaccgc aactttacct tcgagagaaa atgcacttaa aatcataaca
    11761 ataccttatt cgtttatgcc gacaactagc gcaaatcttt aacggccact atgccgacaa
    11821 ctagcgcaaa tctttaacgg ccacatggtc catatcatca aagacctggt tttcaccaac
    11881 catgccccag ataaaggtat aagctttggt tccgacgccg gaatggatcg accagctggg
    11941 ggagatcacc gcctgctcgt tatgcatcac aatgtgacgc gtttcttgcg gctgccccat
    12001 catgtggaaa acgcaggcgt catcatccat attgaaatag aaataaactt ccatccggcg
    12061 ctcgtgagtg tgacacggca tggtgttcca caagttacct ggagccagct ccgtcagccc
    12121 catactcaat tggcaggttt ccagcacatc cgggacaaaa tatttgttaa ttgtgcgacg
    12181 gttactggtg aggttatcgc ctaacgtgac tggagatact tcgtccggtg tgactttttt
    12241 ggtgggatac gtcgtatgcg cgggtgcgca attgtaataa aactttgccg gagtggcggt
    12301 atcaatactg gcaaagacaa cttcttttgc acctttacca acatacaggg cgtcgcggtg
    12361 accgatttca tagcattggc catcgacagt aatcgtaccg gcaccgccaa tattgataac
    12421 acctaactcg cgacgttcaa ggaaatagct tacgccgagt tgtttaccaa cttccccgcc
    12481 aacggaaacc gttttagtta tcggcataat gccgccaaca ataattcggt caatgtggct
    12541 gtaaaccatg gtgtactcat cggcgacaaa taccttttca accaaaaatt cattgcgcag
    12601 cccttgggta tccagcgttt ttgcgtgcgc actgtggatg ccctgtctta cgtccacatc
    12661 aacctccgat aatcagtgaa gcgtgaaaat agaaaacgat agtgaaacac tgtttcgttt
    12721 tctctataaa gggatgatat gcccatgggc tagggttttc aattaaaaat aaaacaatgt
    12781 ttcattttta agttaaggat taaaaaaagt gcagtgtcga tcacgaatat gtcatgagaa
    12841 tggaaaaatg agggagccca gaaaatgtat gtcgacttgc cagccatcat tattcaacag
    12901 attaatgacg gcagatattt atcggtaagg aggaaatgca gagctactcg tcacgttcaa
    12961 tggtcaatgc cacgccctgg cccccgccga tacaaagcgt tgccagccct ttgcgggcat
    13021 tacgtttcac catttcatga accagagaaa ccaggattcg gcaaccggaa gcgcctatcg
    13081 ggtgaccgag tgcgatcgcg ccaccattga cattgacccg acgctcatcc cactcaagca
    13141 tcttgccaac cgaaagcgcc tgtgcagcaa acgcttcatt agcctcgata agatcgacat
    13201 cagccaactg ccagcctaca cgctccaggc aacggcgggt cgcatacacc ggcgcaattc
    13261 ccatcaatgc cggatctaca ccaacgctgg caaatgcgcg gatgcgggcc agcacgggta
    13321 aattcaacgc tcgtgctttg gcttcgctca tcatcattac cgcagctgcg ccatcgttta
    13381 tggatgatgc attccccgcg gtcacagaac cgagactatc aaatgaagga tttaaacggg
    13441 ctaaaccttc tgcactggcg tcagtgcgtg gctgttcatc ggtatcaaca accaacgtct
    13501 gaccgttact ttggatcatt accgggacga tctcatcttt aaatcgtccg gcgtcaatcg
    13561 ccgctcgcgc tttttgttgc gagctaagtg cgtaagcatc ctgcaactga cggctgatgc
    13621 catattcgcg agccagattt tcggcggtga caccaatatg ataatcattg aaggcatccc
    13681 acaacccatc atgcaccaga ctgtcaacca actggctatt gccaagctgt gcaccggtgc
    13741 ggctatcagt cagaacatgt ggtgcgcggc tcatgttttc ctggccaccg gcgatgacaa
    13801 tatcagcctc gccacactgt atcgcctgag tagccagatg cagtgcttta agtccggaac
    13861 cgcaaacgtc attaatcgtg attgcagaaa cgctattagg aagaccacct ttaatagccg
    13921 attgccttgc cggattctgc cctgcccctg cagtcaacac ctgaccaaga attacttcat
    13981 ccaccgcata tgcaggaacg ccggtacgtt ctattaacgc tttcacgacc agactaccaa
    14041 gttccacggc ggaatgaccc gctaacgcac cacgaaagca gccgataggt gtccgtaacg
    14101 ccccgacaat cacaacgtct ttcatcacaa cctcgcacca attaacagtg caatagtaaa
    14161 tgattgttaa ttacagttat cttaattgtt taaaaaaagt gatttttatc acaaaggaaa
    14221 tatgcctgag cagtagtcag agtcataact ggcacgtaag gtttgcaacc actaacccac
    14281 caatagatgg gtagataagg cgttaatctc ccatacttaa cctggtttat ggtaaattgc
    14341 cctccatttt gtttaatttg tagatgatac gttcagataa tgtctaatat ttggtctaaa
    14401 gaagaaactc tgtggagttt cgcgctctac ggcacagccg ttggtgcagg cacgctcttc
    14461 cttcctattc agttaggttc ggcaggggct gtggtcctgt ttattactgc tctggtcgcc
    14521 tggcctttaa catattggcc acataaagcc ttatgccagt tcatcctctc atcaaaaaca
    14581 tcagcatgtg aagggataac gggcgcggta acacactact atggcaagaa gattggtaat
    14641 ctgattacca cgctgtactt catcgccttt tttgtcgtcg tgttgatata tgcagtggca
    14701 attaccaact cacttacgga acagctggca aagcatatgg ttattgatct tcgcatccgt
    14761 atgttggtga gtctgggtgt tgtattaatt ctgaatctca tttttctgat gggacgtcat
    14821 gccactattc gggtaatggg atttttggta ttcccattga ttgcctattt cttatttctt
    14881 tccatttacc ttgtcggtag ttggcaacct gatctattaa cgacccaggt agagttcaat
    14941 cagaataccc ttcaccagat atggatatcg attcccgtga tggttttcgc ctttagccat
    15001 acgcccatta tttctacgtt tgccatagac agacgtgaaa aatatggcga acacgctatg
    15061 gataaatgca aaaaaattat gaaagtcgct tatctcatca tctgcataag tgtactgttc
    15121 tttgtcttta gctgcctgct ttctattcca ccttcgtata ttgaagcggc taaagaagaa
    15181 ggggtcacca ttttatcggc gctttctatg ctgccgaacg ccccagcatg gttgtcaatt
    15241 tccgggatta ttgtcgcagt agttgcgatg tcgaaatcat tcctgggtac gtactttggc
    15301 gttattgaag gtgccacgga ggtcgtcaaa acaacactac agcaggttgg tgtaaagaaa
    15361 agtcgtgcat ttaaccgcgc actatcaatt atgttggtat cgctgattac cttcattgtt
    15421 tgttgcatta acccgaacgc gatttcgatg atttatgcga tcagcggccc gctcattgcc
    15481 atgatacttt tcatcatgcc tacgttgtca acgtatctga tcccggcgct taaaccctgg
    15541 cgctccatcg gaaatctgat tacgctgatc gtgggtatcc tgtgcgtatc ggtaatgttc
    15601 tttagctaat atccgttgtg gatggttcaa aattgctaca attttgaacc attccatttt
    15661 tcataatatt catttgttat tattccgccc tatcacttct ctcgtcatat cctagaatta
    15721 tcagttagtt aaacacacaa agagatctga acatcttaaa taagactatt taagatgcat
    15781 aacttagatt cgcaagatat cttctggtca ttcaaaaaca atttgcgata tagatattct
    15841 cataccatga gatagttatc tggaccacgt tcgaccatgc aagattaaat cagtgaaaca
    15901 taatcatatt taataacgag aaatgcattt ttaaatgtct taatcagaat tatagcaaat
    15961 acagattaat cgcaattttt cccagcaaaa ttacaaaatc aagatgtatt gacatcatca
    16021 atcaccaatg aaattatgca attatatacg gatagggagg ttcttaacat ggggctttgt
    16081 agtcgttata aaagtcttac atgcaatagt tgctcaatgc attgccaaat aatgccagaa
    16141 gagtcaccgc gtttgcagta ttgcgcaaac tcgtgttttt gtatgtggcc cgaagaaagt
    16201 tcatatttta atcgtggtgt ggtggaaggt atcttaacaa aaaaccacaa tgcgagatta
    16261 agcggatata tttttgtgga tttttcagta agttttttac gtctattcct tgaaaaagac
    16321 tggattgact atcttgcaag tactgacatg gggatagttt tagtcagcga ccgcaatatg
    16381 caatcgttag ctaactactg gcgaaaacac aacaccgcaa tatctgctgt catatataat
    16441 gatgatggtc ttgatgttgc caatgaaaag atcagacaac tatttattgg tcgttattta
    16501 tcatttacca gaggtaacac attaactcag atggaattca ccataatggg gtatatggtt
    16561 tctggttata atccatatca gattgctgaa gtgctggata tggatatccg tagcatctac
    16621 gcgtacaagc aacgaatcga aaagagaatg ggtggtaaaa taaacgaatt atttattcgt
    16681 tcacattcgg tccaacattg atactcaacg accagccaga atcatactct gttcatacgg
    16741 gcatctattg aaatgcccgt attttataaa taagcagtaa ccccaagagt aaacaacata
    16801 gtatttaaaa ccatcacacg aaaaattcag aagcattacg aaatatgaat ttacaaacaa
    16861 tagtggcata aatgttaacc atgttaattt acgtaaagtt ttacgttgca acattaaagc
    16921 ctcatttcaa tcatcatgat aaatataaaa ttaatatata tttatgccgt aaataccaat
    16981 atacttagca aactatgtga tctccatttc gattgattta gtgtttattg acgtatgtac
    17041 tggattatta acgataatat cgagttctgg cctgagcacc gaaaattaat atcggtacat
    17101 aacgccgatc ttaacgtcgt tctgacaacg ccagccagtc gatgtttatc acttctactt
    17161 gaagcttttc ctgatgtggt tacacaacaa gattttttca ccagagtctg ggaagaagaa
    17221 ggtatgcgtg tgcctactaa cacgttatat cagaacatat ccattatcag acgcggattt
    17281 cgcgctgttg gtgatactac ccactcgcta attgcaaccg tgccgagaga ggattcaa
//
LOCUS       DAZPLI010000080        17201 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZPLI010000080 DAZPLI010000000
VERSION     DAZPLI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17201
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.081"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(31..1860)
                     /gene="glmS"
                     /locus_tag="ACHUU3_002548"
     CDS             complement(31..1860)
                     /gene="glmS"
                     /locus_tag="ACHUU3_002548"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR2082576.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="ACHUU3_002549"
     CDS             complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="ACHUU3_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR2082577.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3743..4162)
                     /locus_tag="ACHUU3_002550"
     CDS             complement(3743..4162)
                     /locus_tag="ACHUU3_002550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR2082578.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="ACHUU3_002551"
     CDS             complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="ACHUU3_002551"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR2082579.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="ACHUU3_002552"
     CDS             complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="ACHUU3_002552"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR2082580.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="ACHUU3_002553"
     CDS             complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="ACHUU3_002553"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR2082581.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="ACHUU3_002554"
     CDS             complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="ACHUU3_002554"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR2082582.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="ACHUU3_002555"
     CDS             complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="ACHUU3_002555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR2082583.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="ACHUU3_002556"
     CDS             complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="ACHUU3_002556"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR2082584.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="ACHUU3_002557"
     CDS             complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="ACHUU3_002557"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR2082585.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="ACHUU3_002558"
     CDS             complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="ACHUU3_002558"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR2082586.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="ACHUU3_002559"
     CDS             complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="ACHUU3_002559"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR2082587.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="ACHUU3_002560"
     CDS             complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="ACHUU3_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR2082588.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="ACHUU3_002561"
     CDS             complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="ACHUU3_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR2082589.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="ACHUU3_002562"
     CDS             complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="ACHUU3_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR2082590.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15345..16337
                     /gene="asnA"
                     /locus_tag="ACHUU3_002563"
     CDS             15345..16337
                     /gene="asnA"
                     /locus_tag="ACHUU3_002563"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR2082591.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRRQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(16342..>17201)
                     /gene="viaA"
                     /locus_tag="ACHUU3_002564"
     CDS             complement(16342..>17201)
                     /gene="viaA"
                     /locus_tag="ACHUU3_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR2082592.1"
                     /translation="IVKYGEFLNEQPELKRLAEQLGRSREAKSIPRNDAQMETFRTMV
                     REPATVPEQVDGLQQSDDILRLLPPELATLGITELEYEFYRRLVEKQLLTYRLHGESW
                     REKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGGFNEQCAKAFCLALMRIALAENRRC
                     YIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGGTDLASCFRAIMERLQSREWFDADA
                     VVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVAMSAHGKPGIMRIFDHIWRFDTGMR
                     SRLLRRWRR"
BASE COUNT         4107 a         4574 c         4310 g         4210 t
ORIGIN      
        1 tcggcagccc ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaggtt
       61 acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca gcagctgcag
      121 cggaacggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg
      181 catgttatcg ctacttacaa aacccgcatc ctgatcggcg aagacataca attggccgcc
      241 acgcgcgcga acttcttcaa tgttggattt cagtttttcc agcaattcgt tgttcggtgc
      301 aacgacgata accggcatat cggcatcaat aagcgccagc ggaccgtgtt tcagttcacc
      361 agcagcgtag gcttcagcgt gaatgtagga gatctctttc agcttcaatg cgccttccag
      421 tgcgattggg tactgatcgc cacggcccag gaacagtgcg tgatgtttgt cagagaaatc
      481 ttctgccaga gcttcaatgc gtttgtcctg agatagcatc tgctcaatac ggctcggcag
      541 cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca tccagacctt tcaggcgaga
      601 cagcttcgcc accagcatca acagcacagt taactgagtg gtgaatgctt tagtggacgc
      661 cacgccgatt tccgtacccg cgttggtcat cagcgccaga tcggattcgc gcaccagaga
      721 agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg
      781 caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt
      841 acgacgcacg gcagatttac gatagcggaa ttcagaggcg atttcgacgt cgcacggaat
      901 acctgccagc gattcaaacc agtagcggga aaccataccg gagttataag aagtaccaca
      961 ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc
     1021 gcttaaatca acctgaccgt ggctgatgcg cccggtaagg gtgtttttta tcgcgttcgg
     1081 ctgttcgtag atctctttct gcatgtagtg acggtaaata cctttatcgc ccgcgtcata
     1141 ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt
     1201 taccgaacgg cgagtgattt ccgcaatatc gccctcttca aggaagataa agcgacgggt
     1261 caccggcaac agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaagccaat
     1321 caccagcgga ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat
     1381 cactgtaccg tacgcaccgc gcagctgcgg gatagcacgc agaacagcct cacgcagagt
     1441 cccgccttgt ttcagctccc agttcaccag atgggcaatc acttcggtgt cggtttcaga
     1501 aacgaaggta tagccacgcg cttttaactc ttcacgcagc ggttcatggt tttcgatgat
     1561 gccgttatgc accaccacaa tgtgttcaga aacatgcgga tgcgcgttag cttctgaagg
     1621 ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg ccgccatcca gaggatgttc
     1681 ttccgctgcc tgagccagca tctggacttt accgaggcga cgcaggcggg tcatatgacc
     1741 ttccgcatca acaacggcca gaccggcaga gtcatatccg cggtattcca gacgacgtaa
     1801 accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat
     1861 agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc
     1921 cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat
     1981 gttatctcct catcccagat gaccggatta cccggccaga attacttttt ctttaccgga
     2041 cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg
     2101 acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg
     2161 gccaccagct gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta
     2221 ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt
     2281 tccgcatcgc ccaggtaagt cagatgcccg actttcgagc ctttacccag acgcgctttt
     2341 ttcatctcaa cgaagttacc aacgtgtgca ccttccagca actcagcacc aggacgcaaa
     2401 cgagcaaacg ggccaatagt acaggccgct gccagattcg cgtcttccac aacggtatac
     2461 ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt
     2521 ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca
     2581 tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg cagcataacg
     2641 cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag acgggagagt
     2701 tgcaggcggt tattcacacc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt
     2761 tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgagca
     2821 ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttagc gatcagaatg
     2881 ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg
     2941 ccggtaactt tgccattttc acgggtgata cgtccataac cggtcggatc atccagtttc
     3001 accgtcagca gaccaatgcc accctgcggt ttagcatcac gcaggcgctg gagtgtttcg
     3061 acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa
     3121 ggtgcggcct gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagtta
     3181 aggttgtcgt ctttcagcgc ctgtttaagc agatcgccgc cgtgaccgta caccaagtga
     3241 acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg
     3301 gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca
     3361 aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga
     3421 atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat
     3481 aaattgttca ataacggatt atccccgctg acgaaagtgc cattttcgac catcgtcacg
     3541 ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg
     3601 ctcttatttc gatcaatgaa taaacgggaa aacaggggtg ttttatatca gcaggatcta
     3661 tgtgaacgct cttcaggacg gatcacgcgt aaaaaaaagc cagcctgttt ccagactggc
     3721 ttttgtgctt ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct
     3781 gcgcgatcgc tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc
     3841 tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct
     3901 ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc aggctgcact tcaagaatgc
     3961 cgccagacag atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag
     4021 gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac
     4081 ccgttacctg gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt
     4141 ccaggtggta agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt
     4201 ttccacagct tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta
     4261 ttcgccttcc atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc
     4321 cggagaaccg gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt
     4381 acgagcacgc gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaagatggc
     4441 gatgatgtct ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc
     4501 gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc
     4561 aacggccggg tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag
     4621 gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc
     4681 ctgtacggag gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat
     4741 ctcttccgcc agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga
     4801 tacttccgta ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg
     4861 accttcgtca cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt
     4921 tcccggcggc tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc
     4981 ggtcatttcg tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac
     5041 agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt
     5101 acctacaccc gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag
     5161 gtcgataact ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga
     5221 aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac
     5281 cggttcaccc agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat
     5341 cgggtgttcg aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc
     5401 gatggtacgc acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt
     5461 accattttgc acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac
     5521 gtcaactacg gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg
     5581 aaataacctg tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg
     5641 ctggcctgac gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg
     5701 tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc
     5761 acgccctgat aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc
     5821 ggatcgggtt cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc
     5881 ggtaacggca gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg
     5941 ctgacaatgt aaagcttgtc cagacggcct tcgtcgtaag cctgcaacat cacttttacc
     6001 ggaccgatca gttcggacag ggaagggtta tcccccatgc cggtgacctg ggcaacaaca
     6061 ttgccgccca cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat
     6121 tgaacgcctt tgtcggtcca ggtcttcatt tccgccagta gttttttgaa caggttaatg
     6181 ttcaaaccac cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg
     6241 tcgcggtctt ccaggtaagg gtgcttatat tccagattac cgtgtgcaag gtgaccaatc
     6301 actttgcgca tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc
     6361 attttggaag cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt
     6421 gcgatcttac tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc
     6481 ctgccctaag gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga
     6541 tgcctttcag cttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca
     6601 tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccaattt
     6661 tcgacagttc aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct
     6721 gcgcaacgga catcggcgca tactgtttct gtttcagcag ttcggtcact ttctgaccgt
     6781 ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag aacgctgcca
     6841 gttcacgata ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg
     6901 tctgtgctgc accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac
     6961 cggcgttgaa caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg
     7021 tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag
     7081 aaccggtttt ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac
     7141 gtgcagcacg ctccagcaga cgagagtgga ggtagaaaac gtcgcccggg aatgcttcac
     7201 gtcctggcgg acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca
     7261 ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc
     7321 aaccggcata cggtgccagg tattgcagtg cagcggattc agacgcggtt gctaccacaa
     7381 cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg
     7441 acgctttctg gccgatagcg acatagatac atttgatacc ggaatcgcgc tggttgatga
     7501 tggcatcgat agccagtgcg gttttacctg tctgacggtc accgatgatc aattcacgct
     7561 gaccacgacc gattgggatc atggagtcaa cggctttata accggtctgt accggctgat
     7621 ctacggactg acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt
     7681 gatccagcgg acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca
     7741 ggccacggcc aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg
     7801 caaggtcagc gtacggaccc ataacaaccg cacctacaga gtcgcgctca aggttcagtg
     7861 cgatagcgta acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc
     7921 cgtgaatgcg gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac
     7981 tcacaacatt gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca
     8041 gttgcatgct ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc
     8101 gtacgctgcc atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact
     8161 tatcgatttt gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa
     8221 ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag
     8281 cctcactcac ggcacgaagg tgaataaact gctccagaac atccgggagc gcgttaagac
     8341 gaccattttc agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac
     8401 aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg
     8461 ccatttgttc gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta
     8521 cactttggtg ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa
     8581 taaattcaga catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc
     8641 gctgttagca gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag
     8701 gatagcaact tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttctgc
     8761 ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag
     8821 aatctgcgag cggcgtttgt tcgcctgctc gatgattacc tgggcttccg ctttcgcttt
     8881 tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc
     8941 ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg
     9001 ccatacgtac ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat
     9061 tgttgcgtta agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt
     9121 taaataaaag caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag
     9181 cgatcatcgg gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag
     9241 gaatcagatc aggttgacgc gctgcgcctt ccaggaattt acccccgagg atgccgatac
     9301 cgatcgcagc accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat
     9361 ccatattcag gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg
     9421 gtaaattaat gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg
     9481 aaggcttgca gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac
     9541 tgtgaccacc acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg
     9601 ttaccgaaca gtcgcaaacc gagtgaaact ggtttggaca gcaggcttac cccttcaagg
     9661 attaagttga caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg
     9721 aagccgccga tgcctttcat tttgatgctg tagaacagaa tcaggataaa tacgcccagt
     9781 gccatagaca gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc
     9841 agtacatgtt cagcaatgta cggcagcagg tcgataggca gtaaatccat caggttcatc
     9901 aggaataccc agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg
     9961 tacatgtctt tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac
    10021 ttacctggca caccgctggt cgcctttttg gctacgctac ggaataaaac caggaacaac
    10081 agacccagca ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg
    10141 gggttttgtg gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg
    10201 tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt
    10261 tgttaattac agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca
    10321 gcggcaagaa taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca
    10381 gaactttgaa agcttcgcca aatgcgaatg tccaggccac ccggcctttc gctggtgtat
    10441 acgcctggtg acgccaggca aatatcataa acaaaacgtt aggcagaaag actgccaggc
    10501 ccccgcttat tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg
    10561 ctatcaccac cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg
    10621 acacagacat cacgtttttc actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa
    10681 ctttctttaa ggcttagagt caagcatcaa aaagcggtca aattatacgg tgcgcccccg
    10741 tgatttcaaa caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat
    10801 acaattgcga cttttccgct aaccctgttc gatcatgaaa aactgtaaat aacgcgtaaa
    10861 cactagtgat aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agtatattta
    10921 tttacttggc aaatgatgcc tttgcaagtt tatgatattt cagtctaaaa acagatactg
    10981 ttttaataaa tgacatttac acaacaaaaa ccacccattg acatttttaa taatgtttta
    11041 acagccaatg atggttctta gcgccgattt ttagcagact gatattttca ctaatgactt
    11101 attttctgct taccaaaaaa agccacgtta tcttgttgat acaaaagagt gaacgtggcg
    11161 ttaaatgtaa ccagttatat cagtagaaaa cttggttgtt gttaacagtc taaccggtca
    11221 attttttatg atttttttga taaaaattaa attttatttg ctttaatcac caccagatga
    11281 cgttcgccat ccagagctgg aacctgaagt ttaaccactg attcgacctg atattcttcg
    11341 ggcaacaaag cgatttcatc ttccggcatt tgccctttca gcgcgtagaa acggccttgc
    11401 tcaccaggaa gatggtggca ccagctcacc atatcgttca gagaggcaaa agcgcggcta
    11461 attacgccat caaatggcgg ctctgaagga aactcttcta ccctgctctg tactggctca
    11521 atattctcca gtttaagctc atgttgcacc tgacgaagga aacgcacgcg tttaccaagg
    11581 ctatccaaca gagtgaaatg ggcttcagga cgcacgatag agagtggaat gcctggcagt
    11641 cctggtccgg tgccgacatc gataaaccgt tcaccttgca gatacggtgc caccacaatg
    11701 ctatcgagaa tatggcgtac cagcatctca ttaggatcgc ggaccgaagt caggttgtac
    11761 gctttgttcc atttatgcag catattcacg taggcaataa gctggttttt ctggtgatcg
    11821 gtaagcgaaa tacctgcgtc tttcagcagt aaggagagtt tgttgagcac ggtgattacc
    11881 tgttcttgat gcgttgcctg gtaagcgggt gcttaccagg catttttaat gcgttatgcg
    11941 ctacgacgca gcataccctg ttttttcagc cacaccagca gaatggagat agccgcaggc
    12001 gtgacgccag aaatacgcga agcctggccg atagaggccg gtttgtgatc gttaagtttg
    12061 gcgatcactt cgttagaaag accggatacc tggcggtaat ccagtgtcgc gggtagcagg
    12121 gtgttctcgt tacgcagctg cttttcgatc tcatcttgct ggcgcgcgat ataaccttcg
    12181 tatttaacct gaatctcaac ctgttccgcc gcctgttcgt ctgtcaacgc aggggcaaac
    12241 ggcgtcagcg tggttaattt ttcataagtc atttccggac gacgcagcag atcttcacca
    12301 ctggcttcac gggaaagcgg cgcagtcagg tgagcattca cttcggctgc agcttccgcc
    12361 gacggggtta cccaggtcga tttcagacgc tgacgctcac gctcgatatt ctcaagtttc
    12421 tcgttaaagc gcgcccaacg ttcgtcatcc accaggccca gttcacgacc gatttcagtc
    12481 aaacgcagat ccgcattatc ttcgcgcagc atcagacgat attctgcgcg cgaagtaaac
    12541 atacgatacg gttctttggt tcctaaagtg cacaggtcat caaccagtac gccgagatac
    12601 gcctgagaac gcgccggagc ccaaccttct ttgtcagcag acagacgggc agcgttaaga
    12661 ccgaccagca aaccttgcgc agcggcttct tcgtaaccgg tagtgccgtt aatctgacca
    12721 gcaaagaaca gcccctggat aaacttgctc tccagcgtcg gtttcaggtc gcgtggatcg
    12781 aagaagtcat actcaatggc ataacccgga cgcacgatct tcgcgttttc catcccctgc
    12841 atggagcgga cgatttgcat ctgcacatcg aacggcaggc tggtggagat accgttcgga
    12901 taaatttcgt tagaggtcag cccttccggt tcaaggaaga tctgatgctg atttctgtcg
    12961 gcgaagcgca tgactttgtc ttcgatcgat gggcagtagc gtgggccgac accttcgatc
    13021 acccctgcgt acattgggct acgatcgagg ttactgcgga tcacatcatg ggttttctcg
    13081 ttggtatgag tgatataaca cggcacctgc tggggatgct gggacgcatt gcccataaac
    13141 gagaataccg gcattgggtt atcgccatgc tgttgcgcca gcacactaaa gtcgatagtt
    13201 cgagcatcaa tacgcggtgg tgtcccggtt ttcagacgac caacgcgcag cggcagttca
    13261 cgcaaacggc gagaaagcgg aatggacggc ggatcaccag cacgaccacc gctgtaatta
    13321 tccagaccga tatgaatttt accgtcgagg aacgtcccaa cggtgagcac gacggctttg
    13381 gcacggaact tcagtcccat ttgggtaaca gcaccgacca cgcgatcgtt ttcgacaata
    13441 agatcttcaa ccgcctgctg gaagatcatc aggttcggtt ggttctccag cgccgtacgt
    13501 accgcctgac ggtagagcac acgatccgcc tgagctcggg tagcgcgaac cgccggtcct
    13561 ttgcttgcgt ttagtatcct aaactggata cccgcttgat cgatcgcttt cgccatcaga
    13621 ccgccgagtg catccacttc ttttaccaga tgtcccttcc caataccgcc gatcgccggg
    13681 ttgcagctca tctgccccag agtgtcgata ttgtgtgtca aaagcagagt ctgttgaccc
    13741 atacgcgccg cggccatcgc ggcctcggtg cctgcatgac ccccgccaat gatgatgacg
    13801 tcaaaaggat ccggataaaa catggtgatt gcctcgcata acgcggtatg aaaatggatt
    13861 gaagcccggg ccgtggattc tactcaactt tgtcggcttg agaaagacct gggatcctgg
    13921 gtattaaaaa gaagatctat ttatttagag atctgttcta ttgtgatctc ttattaggat
    13981 cgcactaccc tgtggataac aaggatccgg cttttaagat caacaacctg gaaaggatca
    14041 ttaactgtga atgatcggtg atcctggacc gtataagctg ggatcagaat gaggggttat
    14101 acacaactca aaaactgaac aaccgttgtt ccttggataa ctaccggttg atccaagctt
    14161 cctgacagag ttatccacag tagatcgcac gatctgtata cttatttgag taaattaatc
    14221 cacgatccca gccattcttc tgccggatct tccggaatgt cgtgatcaag aatgttgatc
    14281 ttcagtgttt cgcctgtctg ttttgcaccg gaatttttga gttctgcctc gagtttatcg
    14341 atagccccac aaaaggtgtc atattcacga ctaccaatac cgattgcgcc aaagcggacc
    14401 gcagaaagat cgggcttctg ttcctgcaat gcttcataga aaggagaaag gttgtccgga
    14461 atatctccgg caccgtgggt ggagctgata atcagccaga tccccgaggc agataaatct
    14521 tccaacagcg gaccgtgcag cgtttcggtg gtaaaacccg cctcttccag cttttcagcc
    14581 aggtgttctg ctacatattc ggcaccgccg agggtgctgc cgctgataag agtgatatct
    14641 gccataaacc gccaccttta ttaagagtgg cgtattgtac gctgtgaacg cgttgggatc
    14701 tacctgtgga aaagtatggg attaaaaaag ccgattatgg cttgatggta cgcatgatcg
    14761 ggttttgcag gacgatcaat gtctcggtgg actgaatttc atcaattgtt tggatcttgt
    14821 tgataagtac atgctggaga gcgtcgatcg aacggcacat tacttttata aagatgctgt
    14881 agtggcctgt tgtgtaatag gcttcagtga cttcagcaag gctttccagc tttgccagcg
    14941 cggaagggta gtctttggcg ctctttaata taatgccgat aaagcagcct acgtcataac
    15001 cgagctgctt cgggctgaca tcaatacgcg ccccggtaat gatccccgcc tgcttcattt
    15061 tctctactcg aacgtgaatc gtccccggac tgacgccaaa ttgtttcgcc agttcggcgt
    15121 aagcggtgcg cgcattgccc attaatgctt ccaggatgcc acggtccaga ttgtcgatca
    15181 gataattttc cataggattt tcttatgcag attgatgatt cattctattt tagccttctt
    15241 ttttaatgaa tcaaaagtgt gttaggcttt ttattgaatg attattgcat gtttgtcggt
    15301 ttttgttgct taatcataag caacaggacg caggagtata aaaaatgaaa accgcttaca
    15361 ttgccaaaca acgccaaatt agcttcgtga aatctcactt ttctcgccaa ctggaagaac
    15421 gtctggggct gatcgaagtc caggcgccga ttcttagccg tgtgggggat ggcacgcagg
    15481 ataacttgtc gggctgtgaa aaagcggtgc aggtaaaagt gaaagctctg cctgatgccc
    15541 agttcgaagt ggttcattca ctggcgaagt ggaaacgtca gaccttaggg caacacgact
    15601 tcagcgcggg cgaagggctg tacacgcaca tgaaagccct tcgccccgat gaagaccgtc
    15661 tttctccgtt gcactcggtc tatgttgacc agtgggactg ggaacgcgta atgggcgacg
    15721 gtgagcgtca attctcgact ctgaaaagca cggtagaggc gatctgggcg ggaattaaag
    15781 caaccgaagc tgcggttagc gaagagtttg gcctggcacc gttcctgccg gatcagatcc
    15841 acttcgtaca cagccaggag ttactgtctc gttatccgga tctcgatgcc aaagggcgtg
    15901 agcgggcgat agcgaaagat cttggcgcgg tattccttgt cgggattggc ggcaagctga
    15961 gcgatggtca tcgccacgac gtgcgcgcac cggattatga tgactggagc accccgtcag
    16021 agctgggtca tgcgggtctg aacggcgata ttctggtgtg gaaccctgta ctggaagatg
    16081 cgtttgagct ttcttccatg gggatccgcg tggatgccga cacgttgaag catcagttgg
    16141 cgctgaccgg tgatgaagat cgtcggcagc tggaatggca tcaggcgctg ctgcgcggtg
    16201 aaatgccgca gaccatcggc ggcggtatcg gccagtctcg tttgaccatg ctgctgctgc
    16261 aactgccgca tatcggccag gttcagtgtg gtgtatggcc agctgctgtt cgcgagaacg
    16321 tcccttctct gctgtaataa tttatcgccg ccagcgtctg agcaggcggc ttcgcatccc
    16381 ggtatcaaag cgccagatat gatcgaaaat gcgcatgatg ccgggtttgc cgtgtgccga
    16441 catcgccacg gcatgaaagc gatgctgatg tacccgctgc agctctttca ctttactcgt
    16501 cacgtcgtca ggcaaccgct gagcgataaa atcagaaatc accaccgcat cggcatcaaa
    16561 ccattccctg ctttgcaagc gttccataat ggcgcgaaaa caactggcaa gatcggtgcc
    16621 gccacgaaac tgctggctta aaaaacggat tgcttgttcg atgccttggg ggcctgaaag
    16681 ctcataacgg acgatctcgg tggaaaatag cataatatag cagcgccggt tttcagcgag
    16741 agcaatgcgc atcaaggcca ggcagaacgc tttcgcacac tgttcattaa agccgcccat
    16801 tgagccggaa gtatccacac agacaataaa cggcccgcgc ggctgttcat cgtaatcttt
    16861 atgtaccacc ggacgttcga tcactttttc acgccacgac tcaccgtgca ggcgataggt
    16921 gagcaactgt ttttccacca gccgacggta aaactcatac tccagttccg ttatccctag
    16981 tgtcgccagt tctggcggca gaagacgtaa aatatcatcg ctttgttgca gaccatcaac
    17041 ctgctcagga accgtcgccg gttcgcgcac catggtgcgg aaggtttcca tctgcgcatc
    17101 gttgcgcggt attgatttgg cttcccgaga acgccccagc tgctctgcca ggcgtttcag
    17161 ttccggctgt tcgttaagaa attcaccgta tttcacaatc a
//
LOCUS       DAZPLI010000081        16990 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZPLI010000081 DAZPLI010000000
VERSION     DAZPLI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16990
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.082"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..223)
                     /locus_tag="ACHUU3_002565"
     CDS             complement(<1..223)
                     /locus_tag="ACHUU3_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR2082593.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYESYIANYPEAG"
     gene            complement(233..784)
                     /gene="ybcL"
                     /locus_tag="ACHUU3_002566"
     CDS             complement(233..784)
                     /gene="ybcL"
                     /locus_tag="ACHUU3_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR2082594.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(953..1285)
                     /gene="emrE"
                     /locus_tag="ACHUU3_002567"
     CDS             complement(953..1285)
                     /gene="emrE"
                     /locus_tag="ACHUU3_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR2082595.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            1466..1594
                     /locus_tag="ACHUU3_002568"
                     /pseudo
     CDS             1466..1594
                     /locus_tag="ACHUU3_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1619..1933)
                     /gene="fliE"
                     /locus_tag="ACHUU3_002569"
     CDS             complement(1619..1933)
                     /gene="fliE"
                     /locus_tag="ACHUU3_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR2082596.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2149..3807
                     /gene="fliF"
                     /locus_tag="ACHUU3_002570"
     CDS             2149..3807
                     /gene="fliF"
                     /locus_tag="ACHUU3_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFR2082597.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            3800..4795
                     /gene="fliG"
                     /locus_tag="ACHUU3_002571"
     CDS             3800..4795
                     /gene="fliG"
                     /locus_tag="ACHUU3_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR2082598.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4788..5474
                     /gene="fliH"
                     /locus_tag="ACHUU3_002572"
     CDS             4788..5474
                     /gene="fliH"
                     /locus_tag="ACHUU3_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR2082599.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5474..6847
                     /gene="fliI"
                     /locus_tag="ACHUU3_002573"
     CDS             5474..6847
                     /gene="fliI"
                     /locus_tag="ACHUU3_002573"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR2082600.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6866..7309
                     /gene="fliJ"
                     /locus_tag="ACHUU3_002574"
     CDS             6866..7309
                     /gene="fliJ"
                     /locus_tag="ACHUU3_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFR2082601.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7306..8433
                     /locus_tag="ACHUU3_002575"
     CDS             7306..8433
                     /locus_tag="ACHUU3_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR2082602.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8538..9002
                     /gene="fliL"
                     /locus_tag="ACHUU3_002576"
     CDS             8538..9002
                     /gene="fliL"
                     /locus_tag="ACHUU3_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR2082603.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            9007..10011
                     /gene="fliM"
                     /locus_tag="ACHUU3_002577"
     CDS             9007..10011
                     /gene="fliM"
                     /locus_tag="ACHUU3_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR2082604.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10008..10421
                     /gene="fliN"
                     /locus_tag="ACHUU3_002578"
     CDS             10008..10421
                     /gene="fliN"
                     /locus_tag="ACHUU3_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR2082605.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10424..10789
                     /gene="fliO"
                     /locus_tag="ACHUU3_002579"
     CDS             10424..10789
                     /gene="fliO"
                     /locus_tag="ACHUU3_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR2082606.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            10789..11526
                     /gene="fliP"
                     /locus_tag="ACHUU3_002580"
     CDS             10789..11526
                     /gene="fliP"
                     /locus_tag="ACHUU3_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFR2082607.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11536..11805
                     /gene="fliQ"
                     /locus_tag="ACHUU3_002581"
     CDS             11536..11805
                     /gene="fliQ"
                     /locus_tag="ACHUU3_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR2082608.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11814..12599
                     /gene="fliR"
                     /locus_tag="ACHUU3_002582"
     CDS             11814..12599
                     /gene="fliR"
                     /locus_tag="ACHUU3_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR2082609.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            12884..13507
                     /gene="rcsA"
                     /locus_tag="ACHUU3_002583"
     CDS             12884..13507
                     /gene="rcsA"
                     /locus_tag="ACHUU3_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFR2082610.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13551..13739)
                     /gene="dsrB"
                     /locus_tag="ACHUU3_002584"
     CDS             complement(13551..13739)
                     /gene="dsrB"
                     /locus_tag="ACHUU3_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR2082611.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13902..14129
                     /gene="yodD"
                     /locus_tag="ACHUU3_002585"
     CDS             13902..14129
                     /gene="yodD"
                     /locus_tag="ACHUU3_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFR2082612.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14427..15242
                     /locus_tag="ACHUU3_002586"
     CDS             14427..15242
                     /locus_tag="ACHUU3_002586"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFR2082613.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(15239..16933)
                     /gene="dgcQ"
                     /locus_tag="ACHUU3_002587"
     CDS             complement(15239..16933)
                     /gene="dgcQ"
                     /locus_tag="ACHUU3_002587"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFR2082614.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
BASE COUNT         4241 a         4360 c         4390 g         3999 t
ORIGIN      
        1 gacctgcttc tgggtaattc gcaatataag attcatatcc acagggaaat aatattatct
       61 gattattatc aacagttaga ctatgcgtct cccaattgat aacttttttt ccctgacgga
      121 tacgacatga agctggcata agaggcttaa ccctgtgaat ctcatgatgt ttatgcatcc
      181 gtatttcttc aatctttagg ttagtcctat cttttgccag catactctta tcctacttta
      241 cctcataaac gggtattatc tcagcggttg caattttatt ggcattaagc atataaccaa
      301 ctaacgctcc gctggaatta gaatccacag gaatcttatc agtttttaga gcccatattt
      361 taaactggta atgatggggt ttatctcctt tgggaggaca tgctccacca aatcccgcat
      421 agccaaaatc atttcggcct tggacagcac cagtcggaag ttttgtacca tcacgtcttc
      481 ctgcatcagc gggcaaatat gttaccgttg ctggaatatt agcaacagtc cagtgccacc
      541 atccgctgcc tgtaggcgca tctggatcat acacagttac ggcaaagctt ttggtgcctt
      601 ctggagcacc agaccaggtt aatgagggcg atgtattacc cccctcacac ccaactccag
      661 aaaatacatg agacgttgta agttgctctc ctgtttttat ttcattacta gtgacctgaa
      721 atgctgcggc ctgcgcagaa aatgttatga atgccaatac acttgaaacg ataagttttt
      781 tcataaaaac ctctttgttg tgacctatcg ttattttatt tgatgtgcat tgatctcatt
      841 atgcaaaaag gcgcaatgat cgcgcaaatg caatcataat tataaagttg ccccgaacat
      901 tagccataat atgcacaaat cagattcctg ttatctcaag gatgattatt ttttaatgcg
      961 gtgcgcttcg tgacaacaag ttaatgacta acacaccggc acaaatcaac atcatgccta
     1021 tgatggctgg caggtccagt cgttggccaa aaaatcccca ggaaagcaaa ctaatcagaa
     1081 cgataccgac tcctgaccag atagcataag caatccctgt aggtatataa gccagtgtct
     1141 gagctaataa ccaaaatgat gcacaatagc aaataattgt gccaacagag ggccataacc
     1201 gtgtaaaccc ctctgaaaac ttcattaagg ttgtaccaat gacctctgca agtattgcgc
     1261 caccaagata aatataaggg ttcatagcat attctttcct gttcatactg gagagaattg
     1321 tactacagtt tgaacacaac tcaccgtttt catcctggtg tatgtgtatc cagggggctc
     1381 tggcgtggca agagataaag gcaggaacaa taatgatcag aaggaacccc aggctaacaa
     1441 aagagttcac tcttcctatg agcaaagcga agacaaaaca tgttatcgcg tttacggatc
     1501 gtggatggca gaaaactttc aggatcaggt atccatttta aaccagaaat tgagtgagtt
     1561 tgccccatcc atgccccacg cggtgagatc ggatgtaatt aacaacaggt tacaaaacct
     1621 acacctgcat ggacatcact tcctgataag ccgccaccag cttattacgc acctgaatcc
     1681 ccatttgcat agaaactgag gctttttgca tatcggtcat cacatcgttt aacgccacgc
     1741 cgggttcacc gagagtgaat ttttctgcct gcgtgcgggc ggctgtttgt gtatcgctta
     1801 tgcgatcgag cgcggcgtgc agctgcccgg caaaactaat ggtcggttgt ggcagtgatt
     1861 cctgcgcacg cgcactcatc gccgtagcct gtaactggct gataacccct tcaatcccct
     1921 gtatcgctga cattttcatc tcccggataa tttctggtag caaagcttac cagtaagtca
     1981 ataagacaaa ggcgctaaat agcaacaaaa aaaacgggtt tattggcgga tagaaaaaaa
     2041 cgaaagcaca aataatggca gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt
     2101 gttccacttt gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact
     2161 gcagcccaga caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca
     2221 ttgattgttg ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa
     2281 gcccccgact accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc
     2341 agccaactga cgcaaatgaa tattccttac tgcttcagcg aagccagcgg cgctattgaa
     2401 gttccggcag ataaagttca cgaactgcgt ctgcgcctgg cacaacaagg tttgcccaaa
     2461 ggcggcgcgg tcggtttcga actgcttgat caggaaaagt ttggtatcag ccagttcagc
     2521 gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc
     2581 ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt
     2641 gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat
     2701 gaagggcaaa ttagcgccat tgtgcacctg gtttccagcg ccgttgctgg tctgccgccg
     2761 ggaaacgtca cgctggtgga tcagggcgga catctgttaa cccagtccaa taccagcggg
     2821 cgcgatctta atgacgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcgg
     2881 cgtattgaag caatcctgtc gcctattgtt ggtaacggta atattcacgc ccaggtcacg
     2941 gcgcaactgg acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat
     3001 gaatctcatg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt
     3061 tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca
     3121 atcagcacgc ctccggcaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat
     3181 agcgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt
     3241 cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat
     3301 tacaaaacct tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt
     3361 gaagatctga cccgcgaggc gatgggcttt tctgaaaaac gcggtgactc gctcaatgtc
     3421 gttaactcgc cgttcaatag cagtgacgaa agcggcggag aactgccgtt ctggcaacag
     3481 caagcgttta tcgatcagtt gctggctgcc ggtcgctggt tgctggtact gctggtggcg
     3541 tggctgctgt ggcggaaagc ggtacgcccg cagttaacac gtcgcgccga ggcgatgaaa
     3601 gctgtacagc aacaggcgca ggcccgtgag gaagtggaag atgcggtgga agtccgcctg
     3661 agcaaagacg aacaactcca acaacgacgc gctaaccaac gtctgggggc agaagtcatg
     3721 agccagcgta tccgtgaaat gtctgataac gatccgcgcg tggtggcgct ggtcattcgc
     3781 cagtggataa ataacgatca tgagtaacct gacaggcacc gataaaagcg tcatcctgct
     3841 gatgaccatt ggcgaagacc gggcggcaga ggtgttcaag cacctctccc agcgcgaagt
     3901 gcaaaccctg agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc agctaaccta
     3961 tgtgctggcg gagtttgagc aagaagctga acagtttgcc gcactgaata tcaacgccaa
     4021 cgattatctg cgttcggtat tggtcaaagc tctgggtgag gaacgtgccg ccagcctgct
     4081 ggaagatatt ctcgaaactc gcgataccgc cagcggtatt gaaacgctca actttatgga
     4141 gccgcagagt gccgccgatc tgattcgcga tgagcatccg caaattatcg ccaccattct
     4201 ggtgcatctg aagcgcgcgc aggccgccga tattctggcg ttgttcgatg aacgtctgcg
     4261 ccacgacgtg atgttgcgta tcgccacctt tggcggcgtg cagccagccg cgctggcgga
     4321 actgaccgaa gtactgaatg gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg
     4381 cggcgtgaga acggcagccg aaattatcaa cctgatgaaa actcagcagg aagaagccgt
     4441 tattaccgcc gtgcgcgaat tcgacggcga gctggcgcaa aaaattatcg acgagatgtt
     4501 cctgttcgaa aatctggtgg atgtcgacga tcgcagcatt cagcgtctgt tgcaggaagt
     4561 ggattccgaa tcgctgttga tcgcgctgaa aggggccgag cagccactgc gcgagaaatt
     4621 cctgcgcaat atgtcgcagc gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg
     4681 tccggtgcgt ctgtcgcagg tggaaaacga acagaaagcg attctgctga ttgtgcgccg
     4741 ccttgccgaa actggcgaga tggtaattgg cagcggcgag gatacctatg tctgataatc
     4801 tgccgtggaa aacctggacg ccggacgatc tcgcgccacc acaggcagag tttgtgccca
     4861 tggtcgagcc ggaagaaacc atcattgaag aggccgaacc tagccttgag cagcaactgg
     4921 cgcaactgca aatgcaggcc catgagcaag gttatcaggc ggggattgcc gaaggtcgcc
     4981 agcaaggtca tgagcagggc tatcaggaag gactggccca ggggctggag caaggtctgg
     5041 cagaggcgaa gtctcaacaa gcgccaattc atgcccggat gcagcaactg gtcagcgaat
     5101 ttcaaactac ccttgatgcg cttgatagtg tgattgcttc gcgcctgatg cagatggcgc
     5161 tggaggcggc acgtcaggtg atcggtcaga cgccaacagt ggataactcg gcactgatca
     5221 aacagatcca acagttgttg cagcaagaac cgttattcag cggtaaacca cagctgcgcg
     5281 tgcacccgga tgatctgcaa cgtgtggatg atatgctcgg cgcaacctta agtttgcatg
     5341 gctggcgctt gcggggcgat cccacactcc atcctggcgg ctgtaaagtc tccgccgatg
     5401 aaggcgatct cgacgccagt gtcgccactc gctggcaaga actctgccgt ctggcagcac
     5461 caggagtggt gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataactttga
     5521 agccaaaatg gcgcagttgc ctgcggtacg tcgctatggg cgattaaccc gcgctaccgg
     5581 gctggtgctg gaagccaccg gattacaatt gccgctcggc gcaacctgtg tcattgaacg
     5641 ccagaacggc agcgaaacgc acgaagtaga aagcgaagtc gttggcttta acggtcaacg
     5701 gctgttttta atgccgctgg aggaagtgga aggtgtcctg cccggcgcgc gggtttatgc
     5761 caaaaacatt tcggctgaag gactgcaaag cggcaagcag ttgccgctcg gtccggcgtt
     5821 attaggtcgc gttctggacg gcagcggtaa gccgctcgat ggcctgccct cccccgatac
     5881 gacggaaacc ggtgcgctga ttaccccgcc atttaacccg ttgcaacgta caccgattga
     5941 acatgtgctg gacaccggcg tgcgcccaat caatgccctg cttaccgttg ggcgtgggca
     6001 gcgtatgggg ctgtttgccg ggtccggcgt tggtaaaagt gtgctgctgg ggatgatggc
     6061 acgttacacc cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgtg ggcgcgaagt
     6121 aaaagatttt attgagaata tcctcggtgc tgaagggcgt gcacgctccg tggtgattgc
     6181 cgctccggcg gatgtctctc cgcttctgcg aatgcaaggt gccgcctatg ccacgcgcat
     6241 tgccgaagat tttcgcgatc gtggtcagca tgtgttgctg attatggact ccctcacccg
     6301 ctacgcgatg gcccagcgtg aaattgcgct ggcgattggc gaaccacccg ccaccaaagg
     6361 ttatccaccg tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg gaaatggcat
     6421 tagcggcggc ggctcgatta ccgcgtttta taccgtgctt actgaaggcg atgaccagca
     6481 ggaccccatt gcagactccg cacgggcgat cctcgacggt cacattgtgc tgtctcgccg
     6541 actggcggaa gccgggcact atccggctat cgatattgaa gcgtcgatca gccgcgcaat
     6601 gacggcgttg atcagtgagc aacattacgc gcgagtgcgc accttcaaac agctgttgtc
     6661 gagttttcag cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag gcagcgatcc
     6721 gatgctcgat aaagccatcg ccctgtggcc gcagctggag ggctatttac aacaaggcat
     6781 ttttgaacgt gcggactggg aagcgtctct ccaggggctg gagcgtattt tcccgacagt
     6841 gtcataaccc aggagataac ggtagatggc agaacatggt gcgctggcga ccctgaaaga
     6901 tctggcagaa aaagaggtag aggatgccgc gcgcctgctg ggtgaaatgc gtcgcgggtg
     6961 tcagcaggcg gaagaacagc tcaaaatgct gatcgattat cagaatgaat atcgcaataa
     7021 cctcaacagc gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt
     7081 tatccagacg ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca
     7141 gaaagttgac attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca
     7201 gacactgcag gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa
     7261 aaagatggat gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg
     7321 cccttgatta ccgccgacgt tgacaccacc acattgcctg gcggcaaagc cagcgatgct
     7381 gcacaagatt ttctcgcgtt gttgagcgaa gcattagcaa gcgagacaac taccgacaaa
     7441 gcggcccccc aggtgctggt ggcaacagat aagcccacga caaaaggcga gctgctgatc
     7501 agcgatattg tttccgacgc gcaacaagcg gatttactga tccctgtgga tgaaacactg
     7561 cctgtcatca acgtcgaaca atccacatcg acaccgttaa ccaccgctca tacgatgacg
     7621 ttggctgcgg tggctgacaa aaatacgaca aaagacgaaa aagcggatga tctgaatgaa
     7681 gacctcaccg caagcctgag cgcccttttt gcgatgttgc cgggttttga caatacgccc
     7741 aaagtgactg atgcgccgtc aaccgtgtta ccggcagaga aaccaacgct cttcacaaaa
     7801 ctgacttctg cgcaactcac aacagcacag cctgatgatg cccccggcac gccagctcag
     7861 ccattaacac cgctggtagc agaagcccag agtaaagcgg aagtcatcag cacaccttca
     7921 ccggtgaccg ctgccgccag cccgctaatc actccacacc agacacagcc actgctcacc
     7981 gtcgccgcgc ctgtcttgag tgcaccgctg ggttctcacg aatggcaaca atcattaagc
     8041 cagcatattt cgctgttcac ccgccaggga caacaaagtg cagagttgcg tctgcacccg
     8101 caggatttag gtgaagtgca aatctccctc aaagtggatg ataaccaggc gcaaatccag
     8161 atggtttcgc cgcatcagca cgtacgcgcc gccctggaag cagcgctgcc ggtactgcgt
     8221 acgcagctgg ccgagagtgg cattcagtta gggcaaagca acatcagtgg cgaaagcttt
     8281 agtggtcagc agcaggccgc ttcccaacaa cagcaaagcc aacgcacagt aaaccatgaa
     8341 cctctggcgg gggaagaaga cgatacgctt ccggttcccg tctctttaca agggcgcgta
     8401 acaggcaaca gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt
     8461 cttttccccg ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca
     8521 caggaagacc gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt
     8581 ggctcccgat tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact
     8641 ggcattcgca tcaggttgcc gctgaagaca aagcgcagca tcgcgtcgtg ccctcaccgg
     8701 tcttctatgc gctggatacc tttacggtca atttgggcga tgcggatcgc gtgctttata
     8761 tcggtataac cctgcgactc aaagatgaag ctacccgctc gcggctgagt gagtatttgc
     8821 cggaagtccg tagccgtttg ctgttactgt tttcgcgtca ggatgctgcc gtactggcga
     8881 cagaagaagg caagaaaaac ctgattgccg agattaaaac cacgctttcc accccgcttg
     8941 ttgccgggca accgaaacag gatgtcaccg acgtgatgta taccgctttt attctgcgat
     9001 aacgacatgg gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac
     9061 agcgaagtca aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac
     9121 gatccgaata cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat
     9181 gagcgctttg cccgccattt tcgtatgggg ctgttcaacc tgctgcgtcg tagcccggat
     9241 ataaccgtcg gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg
     9301 ccgaccaacc tgaaccttat ccatctgaaa ccgctgcgcg gcacagggct ggtggtgttt
     9361 tcaccgagtc tggtgtttat cgccgtggat aacctgtttg gcggcaatgg acgcttcccg
     9421 accaaagtgg aaggtcgcga gtttacccat accgaacagc gcgtcatcaa ccgcatgtta
     9481 aagctggcgc ttgaaggcta tagcgacgcc tggaaggcga ttaatccgct ggaagttgag
     9541 tacgtgcgtt cggaaatgca ggtgaaattt accaatatca ccacctcgcc gaacgacatt
     9601 gtggttaaca cgccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgt
     9661 ctgccattca gcatgatcga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac
     9721 tcgcgtaatg aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag
     9781 ctggagctgg tcgccaactt tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg
     9841 aaacccggcg acgtcctgcc gatagaaaaa cccgatggca tcatcgccca tgttgacggc
     9901 gtcccggtgc tgaccagtca gtacggcact ctcaacggtc agtatgcgtt acggatagaa
     9961 catttgatta acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacatga
    10021 ataatccggc cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg
    10081 aacaaaaatc aaccagcagc aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg
    10141 gtgatgtcag cggaacgttg caggatatcg acctgataat ggatattccg gtcaagctga
    10201 ccgtcgagct gggccgtacg cggatgacca tcaaagagct gttgcgtctg acgcaagggt
    10261 ccgtcgtggc gctggacggt ctggcgggcg aaccactgga tattctgatc aacggttatt
    10321 taatcgccca gggcgaagtg gtggtcgttg ccgataaata tggcgtgcgg atcaccgata
    10381 tcattactcc gtctgagcga atgcgtcgcc tgagccgtta gtgatgaata accacgctac
    10441 tgtgcaatct tccacgccgg tttctgctgc gccactgctg caggtcagcg gcgcactcat
    10501 cgccattatt gccctgatcc tcgctgctgc ctggctggta aaacggttgg gatttgcccc
    10561 gaaacgcact ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga
    10621 acgggttgtg gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca
    10681 aatcaatctg ctgcataaac ttcccccttc agcaccaaca gaagagatac cgcagaccga
    10741 ttttcagtcg gtcatgaaaa atttgcttaa gcgtagtggg agatcctgat gcgtcgtttg
    10801 ttgtctgtcg cacctgtcct tctctggctg gttacgcccc tcgccttcgc gcaactgccg
    10861 ggtatcacca gccagccgct gcctggcggt ggacaaagct ggtcgctccc ggtgcagacg
    10921 ctggtgttca tcacctcgtt gacgtttatt ccggcaattt tactgatgat gaccagtttc
    10981 acccgcatca tcattgtttt tggtttattg cgtaacgcgc tgggaacgcc ctccgcgcca
    11041 cctaaccagg tattgctggg gctggcacta tttttgacct tttttattat gtcatcggtg
    11101 atcgacaaaa tttatgtcga tgcgtaccag ccattcagcg aagagaaaat atcaatgcag
    11161 gaggcgctgg aaaaaggggc gcagccgcta cgtgagttta tgctgcgtca gacccgtgag
    11221 gcagatttag ggttgtttgc cagactagcg aataccggcc cgttgcaggg acctgaagcc
    11281 gtgccgatgc gcattttgct cccggcctac gtgaccagcg aattgaaaac cgcatttcag
    11341 ataggcttca cgattttcat tccttttttg attatcgacc tggtgattgc cagcgtgttg
    11401 atggcattgg ggatgatgat ggtccccccc gccaccattg ctctgccctt taaactgatg
    11461 ctgtttgtac tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac
    11521 agctagagag gcaaaatgac acctgaatcg gtcatgatga tggggactga agcgatgaaa
    11581 gtcgcgctgg cactggctgc cccgctgttg ctggtggcgc tggtcacggg ccttatcatc
    11641 agtattttgc aggccgccac gcagatcaac gaaatgacgc tgtcgtttat tccaaaaatc
    11701 atcgccgtat ttattgccat tattattgcc ggaccgtgga tgctcaatct gttgctggat
    11761 tacgtccgca ccttgttcac taacctgccg tatatcatcg ggtaggccgt acaatgatgc
    11821 aggtgacaag cgatcaatgg ctttcctggt taagcctgta cttctggccg ttactgcgcg
    11881 tgctggcgct catttctacc gcaccaattc tgagcgaacg cagcgtgccg aaacgggtaa
    11941 aactggggct ggcaatgatg atcacgttcg tcattgcccc atcattacct gccaacgatg
    12001 ttcctgtttt ttcgttcttt gctctgtggc tggccgtgca gcagatcctg atcggcattg
    12061 cgcttggttt taccatgcaa tttgcctttg ccgctgtgcg aaccgctggc gaaattatcg
    12121 gtctgcaaat ggggctgtca tttgcgacgt ttgtcgatcc ggccagccat ctcaatatgc
    12181 ccgttttagc gcgtatcatg gatatgctgg cgttactgct gttcctgaca tttaacggtc
    12241 atttatggtt gatttcactg ctggtcgata cctttcacac cctgccaatt ggtggcgaac
    12301 cgttgaacag caatgcgttt ctggcactca ccaaagcggg gagtttgatt ttccttaacg
    12361 ggctgatgct ggcgttaccg ctcattactc tgctgctgac cctgaatctg gcattaggtt
    12421 tactgaatcg tatggccccg caattatcca tttttgttat tggatttcca ttaactctga
    12481 ctgtcggtat ctctttaatg gcggcattaa tgccgttaat tgcacctttt tgcgaacatt
    12541 tattcagtga gatgtttaat ttgctggctg atattattag tgaattgcca ttaatataat
    12601 tccgtaacgt ttatcatgtt atcctaagga ttatccgaaa aataatacct acgaacatct
    12661 tccaggatac tcctgcggcg aaatatttgt tttaagctca ctcacatatc gcaacattta
    12721 ctttaagaca attccaggca aattatacaa cactttacgg gatagtaagt ccgcctgaaa
    12781 aatcgcgaga gtggcgcatt aggtgaccca tgttgttccg tttagtcatg atgaaatatt
    12841 caggtaaggg gaattatcgt tacgcattga gtgagggtat gccatgtcaa cgattattat
    12901 ggatttatgt agttacaccc gactaggttt aaccgggtat ctgttgagta gaggggttaa
    12961 aaaaagagaa atcaacgaca ttgaaaccgt tgatgacctt gccatagctt gtgattcaca
    13021 gcgcccttca gtggtgttta ttaatgagga ctgtttcatc catgatgctt ctaacagtca
    13081 gcacatcaag cacatcatta atcaacatcc caatacgtta tttatcgttt ttatggcaat
    13141 tgccaatgtt cattttgatg aatatctatt ggtcagaaaa aatttattga taagttctaa
    13201 atcgattaaa ccggaatctc tcgacgatat ccttggcgat attctgaaaa aagagacaac
    13261 gataacctct tttttaaata tgccgacgtt atcattgagc cgaaccgaat cgagtatgtt
    13321 gcgaatgtgg atggcaggtc agggaaccat tcaaatctct gaccaaatga atatcaaagc
    13381 caagaccgtt tcatcgcata aaggtaatat taaacgtaag atcaaaacac ataataaaca
    13441 ggttatctac catgtcgtcc gactgacgga taatgtgact aatggtattt ttgtcaacat
    13501 gcgctaacac attctgactg gtggtttccc accagtcagg ctgaataaga ttactctgct
    13561 ttctccacaa agataccgtc ctgatgccct gcttcattaa agaaccagat gccgagcggg
    13621 tagtcttcca gcgaaaccag gtacattgtg ccttcactaa actcctcaac tgccagtacc
    13681 acgccaggac gacgcggacc gccatccgtt ttgactgtta cccgatcatt caccttcatc
    13741 gttttcctcc tgtggctttg tgccagtgta gaacaatttc gtttttctgg cagctccagg
    13801 cgcgcgcgag tgctgatttt ctcgacggtc tatacttaag agatgccagg cggacttaac
    13861 gactggcggc aacaacagag taacggttgc gaggaaagat gatgaaaacc gcaaaagagt
    13921 acagcgatac cgcaaaacgt gaggtcagcg tcgatgtcga tgccctgctg gcggcgatca
    13981 atgaaattag cgaaagcgaa gttcatcgca gccagaacga ttctgaacac gttagcgtcg
    14041 atggacgtga atatcataca tggcgtgaat tggcggatgc cttcgaactg gatattcatg
    14101 acttcagcgt ctctgaagtg aatcgttgaa tgcacaataa aaaaatcccg accctgaggg
    14161 ggtcgggatg aaacttgctt aagcaagaag cacttaaaaa attcgttaca ccaggaaatc
    14221 tgatgtgttc atcaccttat ccgcaatttt tttcgctgac aagaaaatat tcgcgatatg
    14281 aataattaac gctacttatg aatagaaatg tgacgtgcgt caccccttgg tggctctggc
    14341 gtcaacggga tcgctcccga aaaaatcatc tttgattatt tgtgctgttt tgagaatcat
    14401 cctggtaagg ggagaactgc acgaccatgt tttcaattca acaaccacta ctggttttta
    14461 gcgatcttga tggcaccctg ctggacagtc atagttatga ctggcaaccg actgccctct
    14521 ggctcagccg tttacgcgaa gcgaatgttc ccgtcattct ctgtagcagt aaaacttcag
    14581 cggaaatgct gtacttgcaa aaaacgttgg ggctacaagg gttaccactg attgccgaaa
    14641 atggcgcagt gatccagctt gctgaacaat ggcaggatat agacggtttt ccacgcatca
    14701 tctcaggtat tagccatggc gaaatcagcc aggttttaaa tacgctacgc gagaaagaac
    14761 attttaaatt cacgactttc gatgatgtcg acgatgcaac catcgccgaa tggacgggat
    14821 taagccgtag ccaggcggcg ctgacgcagc tgcatgaggc gtcggtaacg ctaatctggc
    14881 gcgacagtga cgagcgtatg gcacaattta ccgctcgtct gaacgaactg ggcttacagt
    14941 ttatgcaggg tgcgcgcttc tggcacgtcc tggatgcctc tgccggaaaa gatcaggccg
    15001 ccaactggat tatcgcgacc tatcaacaat tatcaggcaa acgcccaacc acacttggcc
    15061 tgggcgatgg gccaaacgat gcgcccttac tggaggtaat ggattacgcg gtgattgtga
    15121 aagggctaaa ccgtgaaggg gtgcatctgc atgatgagga tccgacccgc gtctggcgaa
    15181 cgcagcgtga aggaccggaa ggctggcgtg aagggctgga ccattttttc tccgctcgtt
    15241 aagcgttatc gctcgcgcat acccgattac gcccggcctg tttagcgaga taaagccgac
    15301 ggtcagccag tgactggagt tgttcaaaat cataatcacc ggtttcctcg ctgctactta
    15361 cccccaacga ggcactgatg cgtatcgtcg tactcttagc aatcaacatc tctttttcat
    15421 ttaacttcag gcgaatacgt tctgcgactt ccgcagcctc cgtcagactc gcgcctggca
    15481 gaatcacaca aaactcctca ccaccgaccc gcccggcaac gtcctgcgca cgcaaggaac
    15541 tgctaattaa tccggcagca tgagaaagaa cacggtcgcc cgcctgatga ccaaagcggt
    15601 cattaatcgc tttaaaatgg tcaaggtcga cctgaatgac agaaaaagga tgttggtgcg
    15661 tctgacacaa tttcgcgagc ggacgggctt tttcaaacag tgcgccacga ttatataaac
    15721 gggttaaggt gtcgtgccac gcctgccact gcaacgagct ttgcagaaca tacatgttgc
    15781 taaccatccg gcgaatcaca taccaggaga tgagtaacat ggtggtaaag agcgcccaca
    15841 gcagggttaa tgcaatgctg atactgccga aatcgccgcg cacgccttcg cttagcgtat
    15901 ggacgcgcac cagcacaccg tcgaaatggt ccagacgttc ccagctaaca tagcgactgt
    15961 ccatacgaat gccgccccgc gtgtcatgtt ccatcgcctg cgccagcaag gccagttcac
    16021 gaggatcaaa aatattccct gttggatggt caggattgga agaggtcaaa aatctcagct
    16081 tactgtcata gagctgatac tcaccatcga ggtttttatc gatggcgttt cttaaaaatt
    16141 gctgcatggt acgcacggga atactcatcc ccagcacgcc ataccagtag ttattactgt
    16201 ctaccggaac actgacggta acctgcggtt cagtattgct ggcgtgttcc ggttgcgaag
    16261 taaaccagcg taccgcgcgg tgacgatttt ctcgttgcga atggccgata aaccagggtt
    16321 gggtgacata gccgtaataa cgcgttggta cattgcgcgt aaacaaggtc ggctgcgtcg
    16381 aaacgtaaaa tccggcacgc gagacataca tcgcctgttc aaccatcgac gaggtgttgt
    16441 gcgccagtcg cagcaagtaa ccaacttcca gtgcagcggt aatttcattg tcgaggcttt
    16501 cattttcgcg agacaggaga ttcccctcgc tgactaatgc atccgatacg ccattgactg
    16561 acagggtgcg tcgtcggttg agttcaattt gccaggcgtg ctcgtcgcga tgctgttcga
    16621 actcggttac agcattacgc agtgaagtga aatccagtgg cgctacgaga gcttcgcgca
    16681 tgccattacg caaaaagatc agtttgtcga cgttatactg caaatgctta tcgagcgcgt
    16741 tggctacgtt ttccagatga ttacgctggc tggagatata ggcatcttcc agcactacca
    16801 cttcacgcca ggtcagcaag gtggaaaaaa gcaataccac gataaagcag agattaacga
    16861 catgaccagg ccccaggcgg cgtgcgagtt ttttcaacca gctctggttt tccatttttg
    16921 tctcgtgctg caccctgacg actcccaacc tgctttttta tgattctggc ataacctggc
    16981 gatagcgtac
//
LOCUS       DAZPLI010000082        16672 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZPLI010000082 DAZPLI010000000
VERSION     DAZPLI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16672
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.083"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            75..1214
                     /gene="arnB"
                     /locus_tag="ACHUU3_002588"
     CDS             75..1214
                     /gene="arnB"
                     /locus_tag="ACHUU3_002588"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR2082615.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1218..2186
                     /gene="arnC"
                     /locus_tag="ACHUU3_002589"
     CDS             1218..2186
                     /gene="arnC"
                     /locus_tag="ACHUU3_002589"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR2082616.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2186..4168
                     /gene="arnA"
                     /locus_tag="ACHUU3_002590"
     CDS             2186..4168
                     /gene="arnA"
                     /locus_tag="ACHUU3_002590"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFR2082617.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4165..5055
                     /gene="arnD"
                     /locus_tag="ACHUU3_002591"
     CDS             4165..5055
                     /gene="arnD"
                     /locus_tag="ACHUU3_002591"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR2082618.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5055..6707
                     /gene="arnT"
                     /locus_tag="ACHUU3_002592"
     CDS             5055..6707
                     /gene="arnT"
                     /locus_tag="ACHUU3_002592"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR2082619.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6704..7039
                     /gene="arnE"
                     /locus_tag="ACHUU3_002593"
     CDS             6704..7039
                     /gene="arnE"
                     /locus_tag="ACHUU3_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR2082620.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7039..7425
                     /gene="arnF"
                     /locus_tag="ACHUU3_002594"
     CDS             7039..7425
                     /gene="arnF"
                     /locus_tag="ACHUU3_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR2082621.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7419..7685)
                     /gene="pmrD"
                     /locus_tag="ACHUU3_002595"
     CDS             complement(7419..7685)
                     /gene="pmrD"
                     /locus_tag="ACHUU3_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR2082622.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(7795..9150)
                     /gene="menE"
                     /locus_tag="ACHUU3_002596"
     CDS             complement(7795..9150)
                     /gene="menE"
                     /locus_tag="ACHUU3_002596"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR2082623.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            complement(9147..10109)
                     /gene="menC"
                     /locus_tag="ACHUU3_002597"
     CDS             complement(9147..10109)
                     /gene="menC"
                     /locus_tag="ACHUU3_002597"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR2082624.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(10109..10966)
                     /gene="menB"
                     /locus_tag="ACHUU3_002598"
     CDS             complement(10109..10966)
                     /gene="menB"
                     /locus_tag="ACHUU3_002598"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR2082625.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10981..11739)
                     /gene="menH"
                     /locus_tag="ACHUU3_002599"
     CDS             complement(10981..11739)
                     /gene="menH"
                     /locus_tag="ACHUU3_002599"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR2082626.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11736..13406)
                     /gene="menD"
                     /locus_tag="ACHUU3_002600"
     CDS             complement(11736..13406)
                     /gene="menD"
                     /locus_tag="ACHUU3_002600"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR2082627.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13495..14790)
                     /gene="menF"
                     /locus_tag="ACHUU3_002601"
     CDS             complement(13495..14790)
                     /gene="menF"
                     /locus_tag="ACHUU3_002601"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR2082628.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14869..15174)
                     /gene="elaB"
                     /locus_tag="ACHUU3_002602"
     CDS             complement(14869..15174)
                     /gene="elaB"
                     /locus_tag="ACHUU3_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR2082629.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15229..15690)
                     /locus_tag="ACHUU3_002603"
     CDS             complement(15229..15690)
                     /locus_tag="ACHUU3_002603"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR2082630.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15755..16672
                     /gene="rbn"
                     /locus_tag="ACHUU3_002604"
     CDS             15755..16672
                     /gene="rbn"
                     /locus_tag="ACHUU3_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR2082631.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLSKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         3848 a         4539 c         4284 g         4001 t
ORIGIN      
        1 aaatggcaaa atatacacaa cactctttat agcaaatata agtggacagg tattcaatgg
       61 cggaaggaaa agcaatgtca gaatttttgc ctttttcgcg accagcaatg ggcgtggagg
      121 aactcgctgc agttaaagag gttctcgaat ccggttggat cacaaccggt ccgaagaatc
      181 aggcgcttga gcaagccttt tgccagttga cgggaaatca gcatgccatc gcggtcagtt
      241 cagccaccgc cggaatgcat atcacgctaa tggcgttgga aattggcaag ggcgatgaag
      301 tgattacgcc ttccctgacc tgggtttcaa ccctcaatat gatttccttg ttgggtgcaa
      361 cgccggtaat ggtggatgtc gaccgcgata cgctgatggt cacgcctgaa gcgatcgagt
      421 cagccattac gccacgcact aaagccatca ttccggtgca ttatgccggt gcgccagcag
      481 atattgacgc cattcgcgcc attggcgaac gttacggcat cgcagttatc gaagatgctg
      541 cccatgccgt cggtacgtat tacaaagggc gacatattgg cgcaaaaggt accgctattt
      601 tttcatttca tgccattaaa aatattacct gtgcggaagg tggcctgatt gtaactgata
      661 atgaaaacct tgcccgccag ctacggatgc tgaaatttca cggtctgggt gtcgatgcct
      721 atgacagaca aacctggggc cgtgcaccgc aggctgaagt cttaacaccg ggctataagt
      781 acaatctgac cgatattaac gccgcgattg ccctgacaca gttagcaaaa ttagagcacc
      841 tcaacacccg tcggcgcgaa attgcccagc aatatcagca agcactggca gctctcccct
      901 ttcagccatt aagccttccc gcctggccgc acgttcacgc ctggcatctg tttattattc
      961 gtgtcgatga acaacgttgt ggtatcagtc gcgatgcgtt gatggaagcg ttaaaagaaa
     1021 gaggcattgg taccgggtta catttccgcg ccgctcacac acaaaaatat tatcgcgagc
     1081 gttttcccac gctgtcgtta ccgaataccg aatggaatag cgaacgcatc tgttctttgc
     1141 cgctgttccc ggatatgact accgccgatg ccgaccgtgt catcacagcc cttcagcaac
     1201 tcgcaggaca ataagccatg tttgaaatcc accctgttaa gaaagtctcg gtggttattc
     1261 ccgtttataa cgagcaggaa agcttaccgg aattaatcag gcgcaccacc acagcctgtg
     1321 aatcgttggg gaaagagtat gagatcctgc tgattgatga cggcagtagc gataattccg
     1381 cgcatatgct ggtcgaagcc tcacaagcgg agaacagcca tattgtgtct attttgctta
     1441 accgcaatta cgggcaacat tcagcgatta tggcgggatt cagtcacgtt actggcgact
     1501 taattattac ccttgatgcc gatctccaga atccgccaga agaaatcccc cgcctggtgg
     1561 caaaagccga tgaaggttac gacgtggtag ggactgtacg ccagaaccgc caggacagct
     1621 ggtttcgtaa aacagcttcg aagatgatta acaggcttat tcagcgcacc accggcaaag
     1681 cgatgggtga ctacggttgt atgctgcgcg cctatcgccg tcatattgtc gatgcgatgt
     1741 tgcactgcca tgaacgtagc acctttatcc cgattctggc gaatatcttc gcccgccgtg
     1801 ccattgaaat tccagtacat catgccgagc gtgagtttgg tgaatccaaa tacagtttta
     1861 tgcgcctgat taatttgatg tacgacctgg tgacctgcct taccacaacg ccgctacgta
     1921 tgctgagtct gctcggcagc attattgcga ttggaggttt tagcattgcg gtgctgctgg
     1981 tgattttacg cctgaccttc ggaccacaat gggcggcaga aggcgtcttt atgctatttg
     2041 ccgtgctgtt tacttttatt ggcgctcagt ttatcggcat gggattgctc ggtgaatata
     2101 tcggcaggat ctacaccgat gtccgcgccc gcccccgcta ttttgttcag caagttatcc
     2161 gtccatccag caaggaaaat gaataatgaa aaccgtcgtt tttgcctacc acgatatggg
     2221 atgcctcggt attgaagctc tgctggctgc cggttacgaa attagcgcca tttttaccca
     2281 taccgataat cccgctgaaa aagcctttta tggttcggtg gctcgtctgg cggcggaaag
     2341 aggcattcca gtttatgcgc cggataacgt taatcatccg ctgtgggtgg aacgcattgc
     2401 ccaactctca ccagaggtga ttttctcttt ttattatcgc catcttattt gcgacgagat
     2461 tttgcagctc gctcccgcag gtgcatttaa tctgcacggt tcgctgttac caaaatatcg
     2521 tggtcgcgcg ccgctgaact gggtgctggt gaacggtgaa acggaaacgg gcgttacatt
     2581 gcaccgaatg gtaaaacgtg ccgatgctgg ggccattgtg gcgcaactgc gcgttgccat
     2641 tgcgccagac gatatcgcta ttacgctgca tcataaattg tgccatgccg cgcgccagct
     2701 actggaacag acattacccg ccattaaaca cggtaatatt ctggaaattg cccagcgcga
     2761 aaacgaagcc acctgttttg gtcacagaac gccagatgac agtttccttg aatggcataa
     2821 accggcatcc gtactgcaca acatggtacg tgccgttgcc gatccgtggc cgggtgcctt
     2881 cagctatgtt ggcaatcaga aattcaccgt ctggtcgtcg cgtgttcatc ctcatgccag
     2941 caaagcacag ccggggagcg tgatttctgt tgcgccactg ctgattgcct gtggcgatgg
     3001 cgcgctggaa atcgtcaccg gacaggcggg cgacggcatt actatgcagg gctcgcaatt
     3061 agcgcagatg ctgggcctgg tgcaaggttc acgcttgaat agccagcctg cctgcaccgc
     3121 ccgacgccgt acccgggtac tcatcctcgg ggtgaatggc tttattggca accatctgac
     3181 agaacgcctg ctgcgcgaag atcattatga agtttacggt ctggatattg gcagcgatgc
     3241 gataagccgt tttctgaatc atccgcattt tcactttgtc gaaggcgata tcagtattca
     3301 ttccgaatgg attgagtatc acgtcaaaaa atgtgatgtc gtcttgccgc tggtggcgat
     3361 agccacgccg attgaatata cccgcaaccc gctgcgcgta tttgaactcg attttgaaga
     3421 gaatctgcgc attatccgct actgcgttaa gtaccgtaag cgaatcatct tcccgtcaac
     3481 ttcagaagtt tatgggatgt gtagcgataa atacttcgat gaggaccatt ctaatttaat
     3541 cgtcggcccg gtgaataaac cacgctggat ttattcggtg tcaaaacaat tacttgatcg
     3601 ggtgatctgg gcctatggcg aaaaagaggg tttacagttc accctcttcc gcccgtttaa
     3661 ctggatggga ccacgactgg ataaccttaa tgcagcgcga attggcagct cccgcgctat
     3721 tacgcaactc attctcaatc tggtagaagg ttcaccaatt aagctaattg atggcggaaa
     3781 acaaaaacgc tgctttactg atatccggga cggtatcgag gcgttgtacc gcattatcga
     3841 aaatgcggga aatcgctgcg acggcgaaat tatcaacatt ggcaatcctg agaacgaagc
     3901 gagcattgag gaactgggcg agatgctgct ggcgagcttc gaaaaacatc cgctgcgcca
     3961 tcacttccca ccgtttgcgg gctttcgtgt tgtcgaaagt agcagctact acggcaaagg
     4021 atatcaggac gtagagcatc gtaaaccgag catccgcaat gcccgccgct gcctggactg
     4081 ggaaccgaaa attgatatgc aggaaaccat cgacgaaacg ctggatttct tcctgcgcac
     4141 cgttgatctt acggataaac catcatgacc aaagtcggct tacgcattga tgtcgatacc
     4201 tttcgtggca cccgtgaagg cgtgccacgt ctgctggaaa tcttgagtaa gcataatatt
     4261 caggccagca tttttttcag cgtcggcccg gacaatatgg gccgccatct ctggcgactg
     4321 gtgaagccac agtttttgtg gaagatgctg cgctcaaacg cggcatcgct ttatggctgg
     4381 gatattttac tggcaggtac ggcctggcca ggtaaagaga ttggtcatgc caatgccgat
     4441 atcattcgtg aagcggctaa acatcacgaa gtcggcctgc acgcctggga tcaccatgcc
     4501 tggcaagccc gtagcggtaa ctgggatcgg caaacaatga tcgacgatat tgcacgcggt
     4561 cttcgcactc tggaagagat tatcggtcaa ccggtaacct gttctgccgc tgcgggctgg
     4621 cgtgccgacc agaaggtgat cgaagcaaaa gaagcgttcc atttgcgcta caacagcgat
     4681 tgtcgtgggg ccatgccgtt ccgtccactg ctcgaatcag gaaaccctgg cactgcgcaa
     4741 attccggtga ccttacccac ctgggatgaa gtgattggtc gggatgtgaa agcagaagat
     4801 tttaacggtt ggttactcaa ccgcatcctg cgagataaag gcacgccggt ttataccatt
     4861 catgcagaag ttgaaggctg cgcttatcag cataattttg tggatctcct caaacgcgca
     4921 gctcaggaag gcgtgacatt ttgcccttta agcgaactgt tatcagagac gttgccgctc
     4981 ggacaagttg ttcgcggaaa tattgccgga cgtgaaggct ggctgggttg ccaacaaatt
     5041 gcgggtagtc gctgatgaaa tcggtacgtt accttatcgg cctcttcgcg tttattgcct
     5101 gctattacct gttaccgatc agcacgcgtc tgctctggca acccgatgaa acgcgttatg
     5161 cggaaatcag tcgagaaatg ctggcatccg gcgactggat tgtgccccat ctgttagggc
     5221 tacgttattt cgaaaaaccc attgccggat actggattaa cagcattggg caatggctat
     5281 ttggcgcgaa taactttggt gtgcgggcag gcgttatctt tgcgaccctg ttaactgccg
     5341 cgctggtgac ctggtttact ctgcgcttat ggcgcgataa acgtctggct ctactcgcca
     5401 cagtaattta tctctcattg tttattgtct atgccatcgg cacttatgcc gtgctcgatc
     5461 cgtttattgc attctggctg gtggcgggaa tgtgcagctt ctggctggca atgcaggcac
     5521 agacgtggaa aggcaaaagc gcaggatttt tactgctggg aatcacctgc ggcatggggg
     5581 tgatgaccaa aggttttctc gcccttgccg tgccggtatt aagcgtgctg ccatgggtag
     5641 cgacgcaaaa acgctggaaa gatctcttta tttacggctg gctggcggtt atcagttgcg
     5701 tactgacggt tctcccctgg ggactggcga tagcgcagcg ggagcctgac ttctggcact
     5761 attttttctg ggttgagcat attcaacgct ttgcactgga tgatgcccaa catagagctc
     5821 cgttctggta ctacgtgccg gtcatcattg ccggtagcct gccgtggctg ggattactcc
     5881 ccggtgcact gtacacaggc tggaaaaacc gcaagcattc cgcaaccgtc tatttgttga
     5941 gctggacgat aatgccgctg ctgtttttct ccgtcgctaa aggtaaattg cccacctata
     6001 ttctttcctg ctttgcatct ctggcaatgc tgatggcgca ttacgctttg ctggcagcaa
     6061 aaaataatcc tctggcgctg cggattaatg gctggattaa catcgctttt ggcttcactg
     6121 gcattattgc cacatttgtg gtctccccgt ggggaccaat gaacacgccg gtgtggcaaa
     6181 ccttcgagag ctataaagtc ttttgtgcct ggtcgatttt ttcgctatgg gcatttttcg
     6241 gctggtacac cttaacaaac gtcgaaaaga cctggtcttt tgccgcgctt tgcccgctgg
     6301 ggctggcgtt gctggtagga ttttcaattc ctgacagagt catggaagga aaacatccgc
     6361 aattttttgt cgagatgaca caagaatcac ttcagccaag ccgctatatt cttactgata
     6421 gcgtcggtgt tgccgcaggt ctggcatgga gcctgcaacg cgatgacatc atcatgtatc
     6481 gccagacagg tgagttgaaa tacggcctta attatccgga tgcgaaaggg agatttgtca
     6541 gcggtgatga gttcgcaaac tggcttaatc aacatcgtca ggaggggatt attacactcg
     6601 tgctttcggt tgaccgcgat gaagatatca acagtctcgc cattccgccc gcagatgcca
     6661 tcgatcgtca ggagcgtctg gtgctgattc agtatcgtcc caaatgatct ggctaacatt
     6721 agtctttgcc agcttgctta gcgttgccgg gcagttgtgt cagaaacagg caacctgctt
     6781 tgtggcgata aacaaacggc gcaaacatat cgtgctgtgg ctgggactgg cgctggcttg
     6841 tcttggtctt gccatggtgc tctggctgct ggtcttgcag aacgtaccgg taggcattgc
     6901 ctacccgatg ttaagtctga attttgtctg ggtgacgctg gctgcagtaa aactgtggca
     6961 cgaaccggta tcgccgcgtc actggtgtgg ggtggcgttc attattggcg gcattgtgat
     7021 cctcgggagt acggtgtaat gggcctgatg tggggattat tcagcgtcat tattgcctca
     7081 gttgcgcaat taagcctggg ttttgcggcg agtcatctgc cgccgatgac gcacttatgg
     7141 gattttatcg cggctctgtt agcttttggc ctggatgccc ggatactgtt actcgggctg
     7201 ctgggttatc tgctttccgt tttttgttgg tataaaacgc tacataaact tgcgctaagc
     7261 aaagcctatg ccttgctcag tatgagttat gtgctggtat ggattgcttc aatggtactt
     7321 cccggctggg aagggacttt ttcgctgaaa gcactactgg gagtagcctg tattatgagc
     7381 gggttgatgc tgattttcct gcccacgaca aaacaacgtt actgagtttt ccctgccact
     7441 ttacactgcc gttcccactc atcggggcta taactgcttg ccgagaggac tttcaccgta
     7501 tgcagctttt cacgattaat acaataaagc gcattctgca aaggcgagag taaatccccc
     7561 actttcacgg cgaagtcact cttcacttcg gcgatcattt ttatcgcgcc gccagcatcg
     7621 cagagcataa gtacatgtct gttatcttgt ttattgcagc acgatttttt gaccagccat
     7681 tccattgcat tatcctgttt gctaagagtt ttcacatcaa tttacagatt aacagataaa
     7741 cctattatca acggcaatgg aatggcaaaa ttgcgaagtg aaactaatga tttcttaatc
     7801 ctggcgttgc acccactctt ttaacgcctg acgtgaaatt ttaataccgc cgtttttcag
     7861 ctccggcggt aaagttagcc agcgcaccgg ttgttgaaaa cgagccagct tatctttcac
     7921 ccattcgcca agatcaacgg tttgctggtc atactccacc accgctaccg gtcgatgacc
     7981 aaactccttg tcggcaacgg ggacgataaa cacctgcaaa accgcaggat gtgcagcaat
     8041 tacgcgctcg acttcttccg gctgaatacc ctctccgcca ctgaagaaca gattgtctaa
     8101 acgtccgaca atggtcagct tgccattatg catctcaccg cgatcgcgcg tagcgtacca
     8161 gccttcgtca ttaaccagtg aaaccagttg cccgttacgc cagtaacctt ctgccatact
     8221 ggcagcccgc agccacactt cattattaac gattttcact tcccgacccg gcagcggcga
     8281 accaacgtct gccaggccgt cggcttcttt cgcacacacc gtggaggcaa actcggtcag
     8341 accatagccg caaaagcaac gaatcccctg ctcgcgcgcc tgttccgtca actcgaccgg
     8401 gatagccgcg ccgccaagta acaccgcttt cagggaaacg gaactgcggt taaccagcaa
     8461 acgccagagt tgtgttggca ccagtgaagc gtgagtacag cctgccagca tttgctccaa
     8521 tggctgttta tcacgcaccg tcatccgcgc accagcgtat aaccagcgcc acataattcc
     8581 ctgaccggag acgtgaaaca gcggtaaaga gagcaaccag tcatcgtgat cgccaaacgg
     8641 aatcagcgat aacacacctt gcgcactggc aagatgggcc tgataagtat ggacagcggc
     8701 tttcggcaaa ccggtagaac ccgaggtcaa cgtcattgag cacagacgcg tcggctgcca
     8761 cgtagcggca tgtgcgcctt caacccgctg aatgtgcagc gacgttaatg ccggaaacgt
     8821 gttttcccca tccggcacca gcgcaaattg cagcgtcaga ttgggcagca attcttcaag
     8881 caacggttgc ggcagctgag ggttcacggg caacacccgc gccccgcatt gcagtaacgc
     8941 cagccaggcg agcagcgttt gcggcgtatt ccacgcccgc aacatcacgc cgctgccctc
     9001 aaccacaccc tgcgccgcaa agcccgtagc taactcatcg acgcgagcac aaagctcgcg
     9061 ccagttgagt tgctcgtcat taagacgtaa ggcgatggct tctccccgca cttgccgcca
     9121 gtgacgccac ggccagtcag agaagatcat aacaactgct ccagtacatc agcgtccact
     9181 aacggcagtg ggctccccgg ccagcgacga acctgctgcg cctgcatcag atccagcgtg
     9241 tccagtcccg gaatggtgtc tggcgttaac caggcagcaa tccgcgccag ttgcgttaga
     9301 cctaagctcg actcaatgga agaactgatc accgccgtca gccccagcgc atgcgccgcc
     9361 tgtacctgct cgcgtacttt ttccagactg cccgtgagcg tgggtttgat aactaccgcg
     9421 cgcacgccct cttcagccac aaaggcaaaa tccggctcgc gcaggctttc atcccaggca
     9481 atggcaatgc cggtttcacg ggcaaacgct cgcgaatcat cgcgggtttt gcacggctct
     9541 tcgagaaacg cgatgcggtg gcgataatcc gggttaacat atttggcaaa ctgctgacct
     9601 ttcagcggtg tccaggcgcg atttgcgtca agacgcaaat gcagatccgg aattgcctcc
     9661 agcaacagat taaccaccat gccgtcgcgc acggcttcgt acaatcccac tttgaccttc
     9721 gccactttct cgcctggcat atcagcaagt ttgaggatca gatcgtcagg atcgccatta
     9781 cacagtggtg ccgcacggta attggccgcc tgcgggagcg tatctgccag ctccgccaat
     9841 gcacagctta cgccaaaggc aacggaaggc atctgcggta gctcgccatc gcctgccagc
     9901 cagttattta cccaagcaag cagcacaatt tgcgcctctt cccaggtttc ctgactgaag
     9961 cccggcagtg gggagatctc cccccaccct tcgcgctcgc cttcacgcag gcaaacatac
    10021 agcccgtcgc gggtttttaa ccgcctgtcg cgcagaacca tccccgcgtc catggggatc
    10081 tgccagcggt atacctgcgc gctacgcatt acggattccg tttaaatttg ctgaaatcag
    10141 gctgacgttt ctggttgaaa gcgttgcgac cttcctgacc ttcttccgtc atgtagaaca
    10201 gcatggtggc gttgcccgcc agctcctgca gacccgcctg accgtcacag tcggcgttca
    10261 gcgcagcttt caggcagcgc agcgccatcg ggctgttttg cagcatttcg cggcaccaac
    10321 ggacggtttc tttttccaga tccgccagcg gtaccacggt gttgacaagg cccatatcca
    10381 gcgcctgttt tgcatcgtac tgacggcaca ggaaccagat ttcacgcgct tttttctgtc
    10441 cgacgatgcg agccatgtag gaagcgcccc agccgccgtc gaaggaaccg actttcgggc
    10501 cagtctgacc gaagatggca ttatctgccg cgatagtcag gtcgcacatc atgtgcagaa
    10561 cgtgaccacc gccgatggag tagccagcca ccatcgcgac aaccggtttc ggacaggtac
    10621 ggatctgacg ctggaagtcc agcacgttca ggtgatgtac gccggaatca tctttatagc
    10681 cgccgtaatc accacgcact ttctggtcac caccggagca gaacgcttta tcgcctgcgc
    10741 cagtcagaat gatcacgccg acgttgtcgt cataacgcgc atctgccagt gcctggatca
    10801 tctcttttac cgtcagagga cggaaggcat tgcgcacctg cggacgatta atggtgattt
    10861 ttgcgatacc gtcggtggat ttttcataac gaatgtcctc aaaaccttcg gagcagtcgt
    10921 gccattcaac cggtgcgtaa agcattgctt catcaggata aatcatattg tgtcctttag
    10981 tcagaaacgc aagatctgcg ccagacttgc gataacgcca gcgggatttt cccgatgcgc
    11041 gttatgtccg gcgcgaggaa tgacatggca gtcggcagcc agttccgccg ccaaggcgcg
    11101 gaatttgctg tcacgttcac cacataaata ataaaacgca aatgtgcggg cgctaaggtt
    11161 agcacgtaaa tcaggctgga cggcgagaga agtcgcctcc agcatggcgg caagggttgc
    11221 gccattattg ttactgcgca gcgccaccag ctcccggcgt tgatcgtcat tgagcgaggc
    11281 aaaaacaggc tgttgatacc agtcggcaaa taccgccgtt aacggttctg tgcgaaaacg
    11341 ctgcgcccat tggcgatcgg aacgctgacg ttccgcgcgt tgttcagcat tttgcaaccc
    11401 aggatgcccg ccttcgacaa caaccccaca aagccccggc atcccctggc aagccgccat
    11461 catcgccacc cgtcccccaa gcgagtaccc caccaaccag aagttaagga tgttgtaact
    11521 aaccagggtt ttacgcagta agtcggtgac atcatcaaat ccatcgacgc taatcgccgc
    11581 cgaaccaccg tgacctggga gataaacata caaccgtgag tagtcggcaa acgcctcgcc
    11641 cacttcttgc cattcgtggc aatcgccgga aaaaccgtgc aaaaacacca gccagggtaa
    11701 acctggtttg ccgtgttttg cctgcgcgtg caggatcata aatggcttac ctgcgccaga
    11761 agttgctgga gcgtttgcgc accatcggtg tcgttaacca ccatttcaat taccgtggtg
    11821 gttggcgtgc gccaggcatc ggcaagtgcc gtttcaagtt cctgccagtt ttgcggacga
    11881 tgatatttca gctcgaacat cgcggcggcg tgctcaaaat ggacgttttg cggcatcaga
    11941 tagaaacgct cgcgctcgct tttcggcgtt ggcagcagcg agaaaatttg cccgccgttg
    12001 ttgttcacca caattaatat cagcggcgcg gaaacctgac gcaataacgc cagcgcgttg
    12061 agatcgtaaa gtgcggagag atcgcccaca atcgccagcg tcggtttgcc gcttgcccgc
    12121 tgaacgccgg cggcagtcga gagcagcccg tcgataccgc tggccccacg gttgctgtac
    12181 accgggtaac ctgccggaag ttgcgaaagc gcatcaatca gacgtaccac caggctgtta
    12241 ccgacaaaca attgcccctg ttcaggcaga tagtcgctga tgcgatgcgc cagttgcgct
    12301 tcgccaaacg catcgcgacg ggcaataacc gcctgcattg cctgttccgc caggcgcggg
    12361 atttcaacgc accagggctg gcgtttttct gccggatgca gctccagcca gtcggcaata
    12421 ttggcaatta agcggcgtcc acgatggtgc gccggatcaa gccgcccttc gatgtcatcc
    12481 acaatccagt actcttcggg ttcacagctt gcctgccatt gcaggagccg tttgcccgtc
    12541 aggctgcttc ccagttgcac cacaatttgc gcctgttgca gttcgctggt cgctttggca
    12601 ttgcctaacc agagatcggc acacggcagc ggctgtccgg tttgcgacag cacgtcgcca
    12661 atcagcggcc agccaagagt ttgcgcccac agtgcaactt ttttgccctc ttccgcactc
    12721 atgcgcccgg caaccaccac gccgcgtttt tgtcgccaga agaaccagtc gcgctgtttt
    12781 tcactttcca gacgaggcgc ttcacgcagc cacggtttgt cgtcctgcca ccagtcgccc
    12841 agccgctgtt gccagctaag cccggtgtcg tccatttcgc catacagcgg ttcggcaaac
    12901 gggcagttga tatggactcc accagaatga agcgtaccga gagcatggtc gatggtagaa
    12961 accagccaac gtgcggggat atcctgggtc gggcgcggca atgaaatact gtgcgtgggg
    13021 tgagaggcga acattcccgg ctgacgaatc gcctgattcg cgccgcagtc aatcagctcc
    13081 ggcgggcgat cggcggttaa caggatcagt ttttctccgg ttaacccagc ttcaatcagt
    13141 gccggataga gatttgctac cgccgtaccg gaggtcacaa tcaccgccac cggctgcttg
    13201 ctgactttcg ccagccccag cgccagatgc cccaacccac gctcatcgaa atgggtgtgg
    13261 tgaatgaatg cggaattctc cgccgccgct aacgttaacg gtgtagaacg cgagccaggg
    13321 gcgatacaga tgtgtctgac gccgtgacgc gttaatgctt ccagaatgac cgccgcccag
    13381 cgtcggttaa atgcgcttac tgacatgagt ttgtccggta tcaatattgc ggctaagtat
    13441 aaggagctac aaaaatagat tattgatatg aatcggtaat gatgcgactc attactattc
    13501 catctgtaat aaagtacgca gccctgccgc tttgttgtcg atttcctgcc actcttgctc
    13561 ggggtcggaa ccacggacaa tgcccgcgcc agcatataat cgcacgacat tgccgctaat
    13621 ttttgctgag cgcagggaaa cgcagaattc gctttgctgt aatgagagat agcccgcaga
    13681 accggcgtac cattctcggg tgaacggttc gtgacgggcg ataaactgtc gcgccagatc
    13741 gcgcggtaag ccagcaactg ctgccgttgg ctgcaactga tgtaaacaga tcacattatc
    13801 cgctttgttg agtgaagtcc agatacagcg gcgaagatgc tgcactttac gcagacgcag
    13861 tacctgcggc ggtaaaacat ccagcgtctg ggtatcggcc tgtaaccgtt ggcagatatc
    13921 ttccaccacc agcatattct cgcgctggtt tttatcatcc gccatcagcc actcacctaa
    13981 ctgctgcgcc tgcttatcat caggatgatt tgctaccgtt cccgccagcg cttcagtacg
    14041 cagcgctttg tcacgccgcc gccataaccg ttccggtgaa gagccaagaa aagcattttc
    14101 gccatcaaag gccatgtaaa aatggtagca attcagattc agtcgacgac tggcagccat
    14161 catcgccgcc gcgttgaccg gacttgcgaa atgcaggtca gttgcccgag cgagcaccac
    14221 tttgtcgagc tcaccttcgg cgatggtttt cgttgccagt tcgattaatt gcgtccagcc
    14281 cgttttgtcc ggccagtgtt gttctcgcgt ggtggttaaa tgtaacccag gcaagggctt
    14341 gatactcacc agtgtggcga taaattctct tgccttaatc gcatcgtgct gaagggagct
    14401 ttcgctgaat agcgtcagcc gcagcgtggc tttaccgcca cagcgtcgcc attccaggcg
    14461 tggtaaaagt aaattgccct gcgacgggtc aaacgcattc agcccccaaa tgcgtaagtc
    14521 ggcgtgttcc gggtgctggc gaagaaaacg ttgtgcctgg tccaacgacg taaaacgggt
    14581 aatcgcgccc aggacggcag cttcttcatc accattacgt tgttgccagt agaattgcgg
    14641 gtatgtttgc tgactggcca gccagctcaa ggcatcaaaa gcgtctttga gagggaaagg
    14701 aatatcgata acccgaatgc cgggtgtcgc cggaatctct tgcgacaaat ggcgcagtag
    14761 attttccagc gccgtagtaa gtgattgcac gcggacctct ccctgttaaa agcctcacat
    14821 tatacggggt actacaaaaa aaatgcagta ccccggtgta gggaggtttt aacggcgtgc
    14881 cagcaacagt cctagtacca gcccaacggc cgcgcccaca ccaattcctt gccagggttt
    14941 ttcgtggacg tagtcatcag cacgataaac tgcctgcttc gcccgatagt aataactgtc
    15001 tgaagcctgg ctaacccgtt ttttcacatc atccagcgct ttttctgcac gcgctttcag
    15061 ctcaacatat ttctgatcgg cgggatcgcc agaggagcgg agcacctctt ccagtgtttc
    15121 actaagcagc gtcaggtcgt catcgatacg tgtatcacca aactgattag acattctcgt
    15181 tctccatttg cgtaaaacct gtcactaact atagacaagg gttgttgatt acgcctgaat
    15241 tacctcgcgt gccatcccaa tgtgtgggat accatcttct tcatagacct ccgtcacagg
    15301 gataaaaccg aaactctggt agaagttttg cagatgcgcc tgcgccccga ggtaaacagg
    15361 cttatcaggc cagtgatgcg tgcaggtgtc gagggtttta ctcatcagtt gctgccccac
    15421 tttttcgcca cgcaacgctt cactgacaat cacccgacca ataacgaccg gctcaagatc
    15481 atcatcactt ttcagaatcc tcgcatatgc caccagttca tcatttttcc aaccgagaat
    15541 atggcgatta tcccccgtca aatcatcacc atcgatatcc tgataagggc aattctgctc
    15601 gacaacaaac accgcgcaac gcagttgtaa taaggcataa agctgggaaa ccgataattc
    15661 agagtgatgc agatcttgcc attcaatcat aatgctctcc ttttagatgt atgccttata
    15721 ctagccggaa tattttttga aacgtgatga actcatggaa ttaatttttt taggtacttc
    15781 agccggtgtg ccaacccgca cgcgcaatgt cacggcaata ttgcttaacc tgcaacatcc
    15841 gacccagagc ggactttggt tgtttgactg cggtgaaggc acccagcatc agctactgca
    15901 taccgccttt aaccctggga aactggacaa gatttttatc agtcaccttc atggcgatca
    15961 tctctttggt ttacccggct tgctgtgcag tcgttctatg tcaggcatta tccaaccctt
    16021 aacgatttat ggtccgcaag gtatccgtga atttgtggaa accgcgctgc ggattagcgg
    16081 ctcatggacc gattatccgc tggaaattgt cgaaattggc gctggcgaaa ttctcgatga
    16141 tggcctgcgc aaagtgaccg cttatccgat ggaacaccca ctggaatgtt atggctatcg
    16201 tattgaagaa catgataaac cgggtgcatt aaatgcccag gcattaaaag ctgctggcgt
    16261 gccgcctggc ccactgtttc aggaattaaa agcgggcaaa acaatcacgc tggaagatgg
    16321 aaggcagatt aacggcgcag attacttagc tgctccagtg ccaggtaaag cgctcgctat
    16381 tttcggcgat accggcccct gcgatgccgc acttgacctg tctaaaggtg tcgatgtcat
    16441 ggtgcacgaa gcgacgctgg atataaccat ggaagccaaa gccaatagtc gcggccatag
    16501 ctctacacgc caggctgcga cactagcccg tgaggctgga gtcggcaagc taatcattac
    16561 ccacgtcagc tcgcgctatg atgacaaagg ttgtcaacac ctgttacgtg aatgcaggtc
    16621 aattttcccg gcgactgaac tggcgaatga tttcaccgtg tttaacgttt aa
//
LOCUS       DAZPLI010000083        16269 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZPLI010000083 DAZPLI010000000
VERSION     DAZPLI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16269
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.084"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(161..976)
                     /gene="yahL"
                     /locus_tag="ACHUU3_002605"
     CDS             complement(161..976)
                     /gene="yahL"
                     /locus_tag="ACHUU3_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFR2082632.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(1219..2268)
                     /gene="yahK"
                     /locus_tag="ACHUU3_002606"
     CDS             complement(1219..2268)
                     /gene="yahK"
                     /locus_tag="ACHUU3_002606"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFR2082633.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(2355..2429)
                     /locus_tag="ACHUU3_002607"
                     /pseudo
     CDS             complement(2355..2429)
                     /locus_tag="ACHUU3_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2645..4027)
                     /locus_tag="ACHUU3_002608"
     CDS             complement(2645..4027)
                     /locus_tag="ACHUU3_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR2082634.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(4037..4987)
                     /locus_tag="ACHUU3_002609"
     CDS             complement(4037..4987)
                     /locus_tag="ACHUU3_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFR2082635.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(5130..6548)
                     /locus_tag="ACHUU3_002610"
     CDS             complement(5130..6548)
                     /locus_tag="ACHUU3_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR2082636.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(6548..8095)
                     /gene="fdrA"
                     /locus_tag="ACHUU3_002611"
     CDS             complement(6548..8095)
                     /gene="fdrA"
                     /locus_tag="ACHUU3_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFR2082637.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(8085..8948)
                     /locus_tag="ACHUU3_002612"
     CDS             complement(8085..8948)
                     /locus_tag="ACHUU3_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR2082638.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(8988..9593)
                     /locus_tag="ACHUU3_002613"
     CDS             complement(8988..9593)
                     /locus_tag="ACHUU3_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFR2082639.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDYTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            9851..10348
                     /locus_tag="ACHUU3_002614"
     CDS             9851..10348
                     /locus_tag="ACHUU3_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR2082640.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            10440..11372
                     /locus_tag="ACHUU3_002615"
     CDS             10440..11372
                     /locus_tag="ACHUU3_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR2082641.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(11414..12502)
                     /locus_tag="ACHUU3_002616"
     CDS             complement(11414..12502)
                     /locus_tag="ACHUU3_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR2082642.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(12644..>16269)
                     /gene="ehaA"
                     /locus_tag="ACHUU3_002617"
     CDS             complement(12644..>16269)
                     /gene="ehaA"
                     /locus_tag="ACHUU3_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFR2082643.1"
                     /translation="GFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIHVKGT
                     SRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNASEKISLGYYDQN
                     SDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKTSEKK
                     ITLNHTDKNATISADIVSDSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGALGITQ
                     NIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYIDVASG
                     AVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFDEFAY
                     TDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSLVTVGKGAHSISTLGFSGGTVDF
                     GALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDDGNSA
                     IKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTSGVNN
                     DGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQTVSL
                     SNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNGSADS
                     LLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSANVLVS
                     HAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAGNVLV
                     VKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLNGIEL
                     IHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDPMPNP
                     EPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGNTYYT
                     DMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSDSWHV
                     GVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQYSWF
                     DNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTWMGVK
                     ADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLHNSKD
                     FSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAMVGIK
                     WQF"
BASE COUNT         3773 a         4391 c         3855 g         4250 t
ORIGIN      
        1 gctggtgctt ttttaagcga agtgtcgccg atcacaacaa aatatcggcg aaaaggcggt
       61 ggaatgccat ttggcatacg gatatacaga tgaaatgatt acctgctttg cgattcactc
      121 tctggtggaa taacaacttg caggtggaac cctttctgaa ctaaagagtg tagcggattt
      181 taacgtgctt atttacagga taatatctgt cacctaattc ctgcactcta ttagatatcc
      241 attcatttaa tcgtgcatca tctctgtatg tttttacgtc cagaacagag agcaataaca
      301 tcaccagtga ttcaggaaaa acagggtaga tattttttaa gtgtaataat aatccatgcg
      361 caagcgcctg cttaatgggc gcgtctgtct ccggaataga cgacatttcg cgaagccatt
      421 tttccacatg catacactct ttccctttag ggaaaagtct cagcaaaggc atatggtgtt
      481 caagaagaat acatttgcaa tctgttcgca ctgaattgat aaactgcact aatttcttaa
      541 gcccggtaaa gcgtccagcc tgcacatttg ctttaatatc aagaaacgca tcatcctggt
      601 catataccca aactgtccct ttttgatttt caagtatgcg aaaaacaaac ttaaccgggt
      661 gatcagggta atccatccag tcataaaatc ctgggaaatc attaaactgc acatttatgg
      721 tgagtgcgtt atcgtctgac cactcacagt ctcgtacggc agccatgaga tgaatgagtt
      781 tgtcgtcatc gactaaatca tcacgacaat cataaggata atcataatat tgtttaatca
      841 aaaccttgaa tatatcgaca caggaaacca gggagctttt gatatcctct ggtaactgat
      901 tgtttttaac cgtattaagt atgctttgtt gcgtatatgg catcaaatta ttgtgcggag
      961 cttttaatga tatcatcgcg atctctctta aataaaaata aacgtcgcag ataataaagc
     1021 gctgattaaa ccaggcacta tcagaaatcg ctcataacta acgtgagaag tattgtgtac
     1081 tggagggagt taatttaaca atttgaattt acgttatagg ttttactgct actttgatat
     1141 tatttcgctc tccttaacta aaaagttaag tggagagcga agttaatatg ttaaaccaca
     1201 gcgtatttat taattttttc aatctgttag tgtgcgatta tcgataacaa aacgatattt
     1261 cacatcacca cgcagcattc gctcataggc ttcattaatt tgatcggccc gaatcatctc
     1321 tatatcagcc acgatgccat gttcggcgca aaaatcgagc atctcctgag tttctggaat
     1381 gccgccaatc atagaatcgg ctatcgcacg gcgtttcatg atcaggttga aaacttccgg
     1441 cgatttatgc ggtgtcgcag gcgcaccaac cagcgtcatg gtgccatcac gcttcagcaa
     1501 ggtggtaaaa tcgtcgagat tatgtggcgc agctactgta ttcaaaatga aatcgaaact
     1561 cttcagatga gccgccatct catcggcatt gcgtgagtta acaacttcat cggcccccag
     1621 ggctttttcc gcttcgcgtt ttgcctcaga agtggtaaat gccaccacat gtgcccccat
     1681 cgcgtgggcc agcttaatcc ccatatgtcc cagaccgccg atgccgacca cgcccacttt
     1741 tttacccggc ccggcctgcc agtgacgtag cggcgagtac gtggtgatcc ctgcacacaa
     1801 caaaggagcc accgccgcca gctgctcttg cgggtgacga atacgcagaa catatcgctc
     1861 atgaacgacg atctgttgtg agtagccgcc cagagtatgg cccggttcgt ccggcgtcgg
     1921 cgagttatag gtgccgatca tgtgatcaca gtagttttcc agcccgtcct cacactcttc
     1981 gcaatgttta caactgtcga caatgcagcc gacaccgacc agatcgcccg gcgcatgttt
     2041 ttctacctga tcaccaacgg ctaccacacg ccccacaatt tcatgacccg gtacgcaggg
     2101 gtaaaccgtc cccgcccact cggaacggac ctggtggaga tcggaatggc agacgccaca
     2161 gtaagcgatt tcgattttga catcattcgg tcccggttca cgccgtgtga tatccatcgg
     2221 ttcaagtggt tgtttagcgg aatatgcacc aacagctttg atcttcattg tgtttactcc
     2281 tgattagcta tgtgtatttg gcgacgcctg atatgtcatc gcggcaaaac gcgtccattg
     2341 aatatagcca atatttaaac cacccgatcc agccccaaca tgccgcgaac ctgaatgaaa
     2401 cagagcagca acgttcccca taaggcgagc gcctataagt gtgatatttg ctatccgtaa
     2461 aagactaaaa tcggcaaaac tgatgatctt ttcacgtaga caaatagtta tctacacaac
     2521 gtcaataagg gcatattcct caaagataat gtccatttct gccgacacta tcatattgag
     2581 gtcgccccac tccctggggc ttaaatctct tacaattcac cgttgcaggc gtttcaaatg
     2641 cgcatcaacc tgccacactc ccccacacca gttgcccttt atggaacgtt gcggttctcg
     2701 gcgagatacg cgccaccgcc tcggcggaac aggaggcgtc caccagcaca aagctggcat
     2761 catcctgcgc ttttggccat acacgctcgc ctttttcatt cagcggcaat acatcgccag
     2821 tggctagaaa cagcgaacgg gagaggttct gttcgttagg acgaatatag agctgcgcgt
     2881 agagattggc tttttccagc atgtcgccca gaccataagg cgaccagtgg tcgataacgc
     2941 tgtcagtgcc agtcatcact tttacgcctt tgtcgtgcaa ctgtttgagc ggcatatgca
     3001 gcgtgccaat cggcaccgtc gaggcgatag aaatttgctg cgccgccatc cgattcgcca
     3061 gcgcatcgac ctgttgttcg ttgagcgtag ccagcgcaaa ggcgtggctg atggtcagct
     3121 tacctttcag ttgcggcgtt ttctctaccg tttcaaccat ataattgatg gctgccacgc
     3181 ccgacggagt ggtttcgtgc aggtgaatat cgacaccttt gtcgtagtcc agcgcaatct
     3241 gaaacatggt gtcgagggat ttttccatcg cgccatcgac actggtcggg tccagcccgc
     3301 cgacgtaatg cgcacccgcc tgcatcgctt cgcgcattaa gggttccgat ttcgacagca
     3361 gcaaaccgtg ctgcgggaag gcgacgattt cacactcgaa gcccgcctga cgccgcgcca
     3421 gcaccgcctg caaattttgc agatttttta ggccggaaac cggttcgata ttgcagtggc
     3481 tgcgggcaat ggtggtgcct ttcgactgca atagatcgat cagtttttcc gcccgctcct
     3541 gcgtatacgg ttgtaactcc ggcagcattt tctgcttgag tttgatcatg tcctggatag
     3601 tggtgcctgc cggacgattg agcgagcgcc acggcccgcc gtagaaggtt ttgtcgagat
     3661 gaatatgcat gtcgcgagtg gtgggtagca tcaacttacc gccagcgtca tagtgcggca
     3721 gcgtggcgtc cggatgctgc ttgttctcgc gcagggcgac aattttgccg tcctgaatct
     3781 ccacggtctg gcgcgccgtg cgggtctgca ccgccacacc gttttcgtag tcaaaaccgg
     3841 tttccagcag cacgttatcg agatagtaat gcgggtcagt gattttcatg gtgttgttcg
     3901 cttcgcagtt tagggtgcca gctaccgccg catgggcgag cggcacatag gtaccaaaca
     3961 gcgcggcggc ggtgaccatc ttaccgctct ggctcagaaa ttcacggcgg ctattgcttt
     4021 ctttcatcct acgtccttac gttttaataa tatgggtgcc cgtttcaccg cgcagcgccg
     4081 caggcaggca ttccggcgtg gtgataatca cttctttgcc gccctgctcc aggaacgtca
     4141 ggctggcgat gatttttggc aacatgctgc cgggagaaaa atgcccttcc tgcatatagc
     4201 gagtcatggt ggcaatatcc acccgatcca gcgcctgctg ctgcggttta ccaaagtgga
     4261 tacagacttt ttcgacgtcg gtggtgatca ccagaatgtc ggcgtgaatt tcacgggcca
     4321 gcagcgcggt agagagatct ttgtcaataa ccgcgtccac gctttggtaa tcccccgctt
     4381 cagtacgcac taccggaatt ccaccgccgc ccgcgccaat tacgacaaaa ccttgttgga
     4441 tcagcgcctt aatggcgggt gcttcgacaa tacgtttcgg ttccggcgag gcgaccacgc
     4501 gacgatagcc ccgtccggca tcttcaacaa aacaccagtc agggtttgcc ttttgtagtt
     4561 tgtcacgctg gctttcactg aagaatgcac caatgggctt ggtgggatgg gcaaaacctg
     4621 gatcgttttt atccacttcc acctgagtta ccacggtgac ggcttttttc tcgccgtgac
     4681 gcgccagtcg gttattcagc gcctgttgga tcagatagcc gatgccgccc tgcgtatctg
     4741 ccacacagtt cgccagcggc gttaagggca gcccttcgcg ctcgtgggca atctccgcac
     4801 ggcgtaaatc cagcccgacc tgcggcccgt taccgtgggt cagcacaatg tcgtaatcgg
     4861 aagccagcat ttccagcacc gtatcggcca cggctttcac cgcctccgcc tgatgctcaa
     4921 tcgactggct ggcgttatct ttgataatgc tgttgccacc aatggcaacg accacaagct
     4981 ctttcatgtt gttatgtccg ctacagaggg tgatgaggtt atgaccaacg ccagactgcc
     5041 tgatgcgcta cgcttatcag gcctacaatt ctcgggccat ccgtaggccg gataaggcgt
     5101 tcacgccgca tccggcataa acatgtaagt cagacgccgt aacgttcgca ccagccgaga
     5161 atggctttct cgaagcaagc gaatggtgga tgcacaatgc ccgcgccaat catgccgacg
     5221 cccgcatctt tatgggcgat ggcggtgttg atcaccggca gaatgccgct gctacccacg
     5281 cgggtgatgt cgattgccga cggcacgccc ataaaaccga gcagcggaat ggtgacgttg
     5341 gggttttcac cgagagtgat ttcacgcatc tgacgggaga aatcgatagc ctcttccacc
     5401 gtgccgccca ccagcgcgac gattgcaggc gcggtcgcca tagcaaatcc accaataccg
     5461 taggtttcgg tgatggcgct gtcgccgata tccagccccg aatcttccgg cttataaccg
     5521 gcaaacatcg ggccgatcac ctgctgcgcc gggccggtaa accattgccc cggcaggccg
     5581 ctgacccgca ggccgaactc gacgccgtta cgcgccatgg tggtgaccac ggtgctgtat
     5641 tcgatgccgt gcgccgcatc catcgccgct ttacacatcg ccatccacgt cgggccggag
     5701 aagtagtcgc tgctggcgac aaactcgaac acttcgcgct gttgctcgac ggaatagcct
     5761 gcctgaatga tccccggcgt cagtgcctga atcagcagcg tcgtcccggc gttattgcgg
     5821 ttatggcact catcgcccat atgcagcgcc tgcgccagca ttaagcgcag atcgatctca
     5881 ccgataattt tcatcgcgtc gcgcagcatt ggcccctgca catcgcgcat ccagttgagg
     5941 cggtcgatca cgctctgatc gttggcaccc atacgcaaaa tcttcgccat ctgctcgctc
     6001 atattggtgt aagcgatgtt gccgtaggtt ttgtttttca cgatgtgcat aaacatcgag
     6061 gccgaggtaa cgcccgccat cgatcccaca cagtcgtgtt cgtggcacgg cgagaaggtg
     6121 atctccccgg aggccgccag ttcagccgcc tcgtcgagat ctttcgccag tccttcgaac
     6181 accagcgctc cggtgaccgc gcctttcatc gcgccgcaca ttttttccca ggtgatcggc
     6241 ggcccggcgt gaagaatggt tttcggcgtc atgcccggca ccacgttaat cgcctgatca
     6301 aaaccaatca gcaccggatg cgactggata atgcgctcca gcgcctgctg gttagcggcg
     6361 gcgattttgt ctgccagcgg cgaatcggca atgttatcca gcgcctgcac cacctgcata
     6421 ttgccctgcc ccggcggcgt ccagtcgagt tgagtcactt ctacatgctg ctttttcagg
     6481 tcatcgctaa acatggcgat gccgacgtta ataacgttca atggttggct aaacagtgac
     6541 tggctcatca ggcttcctcc cctttgcaga taaattcacg cgccagcaat ccggtattgg
     6601 tgctgctgct cgccagaatc actccggcat caagcagcat ctggctttgt tgttctaacg
     6661 atggcgtatc aagatcggta cccagcacat aggcgagaat gatcaactcg cgtccttcgg
     6721 cagcggcgat cgctttcgct tctttgatcg cctcgatggt ggagccgacc ggatcttcat
     6781 gcgatccaaa tccgagcaca aaatccatca cgatcaccgc cacttctgga tcgcgcgcct
     6841 cttcgatcaa gcgactgatg cggttggtgg ggtcaatcat tggatgcggc ttgccgttcg
     6901 tgaagtcgtc atcgccaaag tcgaggaagg tgtgtttgat gctgcggttg atatcttgca
     6961 ggcggaattc cggatcgggc tgaatgttgc tgtagacatc gccatgtttt tccatcaccg
     7021 cgaacatggt ttcgtcgcac agcgtgccgc cgcagaacag gccacgaatg tatttctgct
     7081 gcggttgcag acgcgcacgc acatcagcaa tcaacggctg gttaagcgta tgcaggtcga
     7141 gattttcctg tttcacgccg gagagcatca ccgcttttag cgccgcctct ttgctgccgc
     7201 gggcaaactg tagcccctgc tcatccaccg gcgtttcaac gcgaccgagg aagcagacca
     7261 ccaccggctt gcggcaggca cgtgcgcgtt ccagcacttt gcgggccacc gcaggcgcgg
     7321 gaggttttga aatcagtgcg ataatttcag tttgcggatc gttttccagc atcccgatgg
     7381 cgtcgagcat catcaggccg ccgattttct cgctcaggtc gcgcccgccg gtgccaatca
     7441 gttgtgaaac accgccacca aattcatgaa tgcggacgct cagctcctgg ctgccggtgc
     7501 cggatgcgcc aacaataccg atattgccgc gacgcacggc gttaccaaaa cagagcgccg
     7561 cgccgttaat aatcgccgtg ccgcagtccg gccccatcat cagcagccct ttttcgtggg
     7621 ccaactgctt gagcgccagt tcatcttcaa ctgagacgtt atcggaaaac agcatcacgt
     7681 tgagatcgtt ttgcagcgcc tgacgcgctt cgcgagcggc aaacagaccg ttgaccgaaa
     7741 tcaccgccag gttactttcc gggatatgct ttttggcgct ggcaatagtg gcgtaacgcg
     7801 cctcgtgcga gccgctttgc gctttggtgt tgaacagttc ttcaatctcc accagcaact
     7861 gctcgttgtc cgcacccgat ttaccattga tgacaatcat caggtcgccg tttttcgcct
     7921 gttccagcgt cggcgtcagc agtcctaaat ttttcagcac acctttgttc atttcggtcg
     7981 ccatcgccac aaatgcctgc tcgacgccgt cgagtttatt tgcacgcgtg gagatagaca
     8041 tcagcgagac agaatcaaaa taggtattcg gtttaatgac tattttaact gacataattc
     8101 cctccgtagg tttggctcag cgcacaacca tcggccatgc catacagcat gtcgcagccg
     8161 gaactggagc caatatgttt aattttttct attgcctgag ttgaattccc agggacgcca
     8221 taaataatgt tgtgaataaa gcgatgaata ttttcccggc agcgttgttg taatgcggct
     8281 tccagcgtta tggcacttaa taacgtggtg ttatttctgc cgcgtagcag accgcgataa
     8341 aattcctctt tgtatttttc tgccggatgc ccgggaataa ataaaataag cgccagacct
     8401 gttaaataat catcggctga tggcgtaagg ccaatcccta acccaatcat actggcgacc
     8461 gctgcggcga tattttgctt atcgtttact gtctgaataa ctgcccgtcg tttaatatgt
     8521 aattctttat ttaacgcctg ataaaacgga ttatcgccgc gataattaaa cagggtttca
     8581 ttttccccca ggtgctgatg aataatgtcg tgccactgta gccaggcgat aaggtgaaaa
     8641 ttcacagcgg tcatttgcaa ggttggggac agccaacgcc gacaacgact catctctata
     8701 tgaagatgtt gaccaaccgt aataccttga tcgtcaaact gaaccttatc accatgccgg
     8761 aacagatcat caaagtgagt gagtgccaac cgacaactgt ttggcgcatt gtcgtactct
     8821 tcacaaagca gcgtcagcaa ctgctggcga gccgggagtg cgatatttac cgctctggca
     8881 aagacctgtt caacctgtcc ttgcccccgc tgcgccagaa aatccgcatc cgcagttagc
     8941 gcccacacac accgtttttc tggtgtgttg atagccagcc tcccggttta tgcacctgcg
     9001 gcaatcagta actgcgcaat ctcttcaaag ccccgttccc gcgccagttc cagtggcgtt
     9061 ttgccatatt tatcggtcag atgcgggctg gcaccgtgtt ccagcaataa ctgcacaatc
     9121 gcctgctgtt taataccacc atcattaagc acaatcgctt ccagtagcgg cgtccagcct
     9181 acatggttgg tctggttaac gttaatctcc gtatgcgcca aaagctcttt tacaatactt
     9241 aaatggcctt tttcacaggc aggcgtcagg ccgacaccgc caaaacgggt tacgcaatta
     9301 agatcgggtt tagccggtaa aataattcgt agtagcgtta aatcatcgtt cagacagcta
     9361 attaaaaaag gatttaaaca ggtataatct tgcttattaa tatccgctcc ggcatcaatt
     9421 aatgcctgaa cgcaagcata ttgctgatat aaacttgcca gcgtaattgc cgttttcccc
     9481 tgacgatcgc aggtattaat atcgacaccc agcgcaaggc aggtttttac tttatcgata
     9541 tcaccctgtt gtgcagccaa taaatagtcg gcaggtagat tttttatact cattcacatt
     9601 cccctggttt ttgtggctgt tttcgatgaa ttgagaatag caacgataat ctgataaaag
     9661 aacccgctta aatttaattc gccgtatctc gcctttttgt tgaataatat tcaacaatct
     9721 tttcgcagtg tgcggtagtg caatatttgt ttctttgacc cgataccctt tcactcaatc
     9781 aggttttatt tctctatagt ctgcacatgt aagagtagct ttcgtccatg tctaaccaga
     9841 ggggaacaag atgaacggac tcacggcaac gggtgtcacc gtcggtattt gtgcagggct
     9901 ttggcagtta gtgtcttcac atgtcgggct ctctcaagga tgggaattat taggaaccat
     9961 tggcttcgtc gccttttgca gtttttatgc cgcaggcggc ggtaaatcgg ggtttatcag
    10021 aagtctggcg gttaattatt ccgggatggt gtgggcgttt ttcgccgcgc tgactgctgg
    10081 ttggctggcc tctgtaagtg gactgtccgc gttctgggcg agcgtaatta ctaccgtacc
    10141 cttctcggcg gtagtggtct ggcaagggcg tttctggtta ctgtccttta ttcccggtgg
    10201 ctttctcggc atgacgctgt tttttgccag cgggatgaac tggacggtga cgttactggg
    10261 ttttctggcg ggtaactgcg tggggataat ttcagaatat ggtggacaaa aattaagcga
    10321 agcgacgact aaacgcgacg ggtattaaaa gttattcaaa aatgattatc tatctttaac
    10381 cgatacctct acagtcatag ctggagaaat tcatagtaga taatggcgag aatggtttaa
    10441 tgaactcaat ttttaccgaa gagaatctgt tggcatttac taccgctgcg cgttttggca
    10501 gcttcagtaa agccgcagaa gagttgggtt taaccacttc cgccattagc tacaccatca
    10561 agcgtatgga gacggggctg gatgtggtgc tgttcactcg cagtacccgc agcattgagt
    10621 taacggagtc cgggcgttat tttttccgca aagctaccga cctgctgaat gatttttatg
    10681 ccatcaaacg cagtatcgat actatttcgc agggcataga agcgcgtgtg cgcatttgta
    10741 ttaatcagct gctttatacg cccaaacaca ccgcacgctt gttacaggtg ctgaaaaagc
    10801 agtttcctac ctgccagatt accgtcacca ccgaagtgta taacggcgtc tgggatgcga
    10861 ttattaataa tcaggccaat atcgccatcg gcgcacccga tacactactg gacggcggcg
    10921 gtattgatta cactgaaatt ggcgcgattc gctgggcttt tgctatcgcc ccggatcacc
    10981 cgttagcctt tgtgccggaa cccatcgccg aaagccaact gcgtctctac cctaatatta
    11041 tggtggagga caccgcgcat acgattaata aaaaagtggg ctggttgctg cacgggcagg
    11101 agtcaattct ggtgccagat ttcaacacca aatgtcagtg ccagatttta ggtgaaggaa
    11161 ttggtttttt gccggattac atggtccgtg aggcgatgac gcaatccctg ctggttaccc
    11221 gacaaatcca taatccgcgc caggattcgc gcatgttact ggcgactcag cattctgcta
    11281 cgggtcaggt gacgcagtgg attaaaaaac aatttgcgcc aaatggcata ttaacgggga
    11341 tctatcagga tttactgcat cgggagaatt aatcgctgcc aacctcccga tgcgctgtga
    11401 atagcggttt acatcaacca cctgctttca ttacccattc agagataaat ttattacccg
    11461 gtactggcgg agagtacaaa tagccctgca aaaagtgaac gcctttaccg atcattaaat
    11521 ccgcctgctc ctgggtttct accccttccg ccacgatact cagaccaggc ttacgcgcta
    11581 gttcgacaat attgtccaca atatgaccgg agatttcgtc gacactcgcc atttgcacaa
    11641 atgacttatc gatcttaata aaatcgaccg ggaacgcctg caagtaacga taggtcgcat
    11701 aacccgtacc aaagtcatcc agcgcaaagg taatgttgtg ctgatgaagg ctgtcaaata
    11761 tcgctctggc ttctggcgtt accggaatag ggttacgttc cgttagctcg agaaccagct
    11821 tgattttatc gttacctaat ttcttaacca ggttcagaca ctctttttca aatcccgctg
    11881 ccaaaaaaca acccgccgag acgttgatgc caatatggaa attgtccggc agcaaatgtt
    11941 ttaccggcat cagaatatcc gcagtctgtt tcatcagttg gcgggtcatt atgacaataa
    12001 gaccggatga ctccgccaga ggaataaact gatccggtgg gataattccc gtttgtggat
    12061 gttcccagcg gacaagtacc tcacagcccg tcagtacccc agtctgcgcg cagaaaaccg
    12121 gttggatcca cggtttgaat tcgtggtttt ccaacgccag actgatggct tcaggcgtga
    12181 cgatatggtg acaacggtta tcagggatat aaaaattatt tttatttccc gtgccactga
    12241 tgacttgcgg atgatattga aaaaagatat cccgccagaa ggtaatatca ctctggatgc
    12301 ctagtttttc atacagctgc ttcttttgat gtgaaattgt ctttgcactg cgatttctgt
    12361 actgtgagat ttctgtaacc gacattccct ccattagcag cttgagtaaa agccgttctt
    12421 tttcggacac gataccaggc aacgtcaaat tcaccgttgc cccgaactct tccaaaaaaa
    12481 cactcaaatc atgcgaagcc attgaacaca ccttgattaa atcttgtgcg ccagctaaat
    12541 ctgcgagtct tcactctgac tgataaagct ggctgcaaga aaatgacaca gagagccgaa
    12601 ttatctcccg ttcggctctc tctcctttta ctcattattc agatcagaat tgccacttaa
    12661 tgccaaccat tgcagaggtg tcattatatc ccctatcggc aacctgaacg cccacattcc
    12721 cccagacatt caggttggca tttagctgtc cttccacccc ggtttttatc tcagcaatat
    12781 ttcgggctcc atcctgagtg acggacacgc catccatact ggtgctgaaa tccttactgt
    12841 tatgcagcca gttcacttct acaaacggct ggaactcgcg ggatttaccg tcatccattt
    12901 tatggtggct cttcagccag gtttttacgc caagtcgggt ttgaacattg ccatcaccgt
    12961 tgctatgaac gagggttccg ttgctttcgc ggtgcttatc ggctttgact cccatccagg
    13021 taacctgtgc ttgcggctga acataccatt cattacgcgt tccctggctg ccattaaatt
    13081 cagctaattt gtgtttgtat ccagcttcca gtgaagcggt aaatcctttt gatttatagg
    13141 attcactttg taagtcatcc cctttcactg tgttatcaaa ccagctgtac tgcgcccaac
    13201 tgtcgagata cgcgccatta cgcgactcgt catcggcata ccaggtggca tagaggccgg
    13261 tgctatatcc gttcacactc gcttttgcac gataaccggt tcgcgaggaa atggttttgc
    13321 tgtcgctgtt gccatatccc gccatgaccc caacatgcca gctgtcgctg ccgttttggc
    13381 tccactgcgc gacatcgcct cccagttgca gaacatagcg attgctttgg gttttcagct
    13441 ggccgctgcc atcacgccat ttattatgac caccttcatg gcgcatccac atagtggttt
    13501 gtttctgctc acccgtcacc atgtcggtat agtacgtatt acccagacgc tcatgcagac
    13561 gcgtggtgaa cattgtattc gctgctgcaa ggttcgcaat gtagctaccc gcctccggtc
    13621 gcaggtcatt atccacattc agatccggac ccggcgtagg tgtcgggtta gggttcggct
    13681 ctggattcgg gtttggctcc ggattcggca tcgggtctgg ctccggctgc agttcaggag
    13741 aatcactgcc gctggtcaga taccagttac cactatttgc cccttgtcca cgcgcgagag
    13801 tgtagtcata cgcccccgca acgatacgac cagcctgaac aaattcgccc tcagacttac
    13861 cgtctacatg gataagttcg ataccgttaa gggttttcgc acctgtaccg ccagcgttat
    13921 tcaccgtaac ggcagtcgtg ccgctagtat cgccctcgac aaccaattta tcggttactg
    13981 agtcatcgcc attcagtacc gtattcatta ctagttgacc gttattaccg tggtagttcc
    14041 ccttcaccac taacacattg ccagcttgct gtcctgacat tccggtcatc agcgtaccgt
    14101 tgttggtcag attgccgccc agggtgtaat tcacagacgc agccgctctt ttttcagcgc
    14161 tattattcaa cgccagggta ccgttgttct caacgtttgc gctacccaat ccctgcgcat
    14221 gacttaccag gacattagcc gaattagcaa tgttcacatt cgctgtcagg ttgctgttat
    14281 cgcccgcgat gtccagcgtg ccgccctgaa tattcagctc tccgctcccc gttaacgaac
    14341 cggttgaagt gcccccgttg gtaaccgtca gactgccgcc atttaagctc agcagtgaat
    14401 cggcgctgcc attgagcgtg cccacggtct ggctgtgacc attcatatcc agttcagtct
    14461 ctgccgccag acgcagttca tgggtattac ccaacacgtt atcgttattc aacaaaagcg
    14521 tcccactgcg cagatcggta acaccggtat agtcgttgtc tttgttagaa agcgatacgg
    14581 tctgaccctt ctggctgctg aatgccaggt caccactgcc ggtaatcttt gcgctgagat
    14641 cggcggcatt ctcgctttta ccgttagagc tcagcaccag agcatcgctg tcggtagcgt
    14701 gtaaatcaag ctgggtcagg ccgtaaccga tatacagacc gtcattgttt accccactcg
    14761 tcagacgata gtcgtaagtg ccgacggccg ctttttgccc attctgctga atatcacgtt
    14821 gggcactgct ggagaggatt tgcccatttt tatcctgcaa ttgcagattg cccgcatcgc
    14881 ccagaacttc cgcaccttgc gcatcaacca gcttaatggc gctgttacca tcatcgactt
    14941 cagtgagcga taacgcagag tcaatatcac gagatactga gcggacaacg tcactgtcag
    15001 aaacctgaat cacaccctcg ccgcgcaaat ccagcgtttt actaacgttg accgtccctt
    15061 ctgtcatctg tgcacctgct gtcagggcac caaaatcaac ggttccgccg gagaatccca
    15121 aagtgctaat ggagtgagcg cccttaccca ctgttaccag gcttccctga cccgcagtga
    15181 tcccgcctct ctgcagaaac tcagcattct tttccaggtt aaatgtcgtg ttctggaacg
    15241 ccagtgaacc ctggaaccag tctgtataag caaactcatc gaaggagaat tcgtgattcc
    15301 ccatgtttat ttcaacaata cctctacctt gaagatcgtt atcaaatacc caatctttat
    15361 tggcattgat ttgcagtttg ccatcaacat ccaggtccgc acggccgatg ttcttgtctt
    15421 cagaaataac cgcgacagca ccagaagcga catcaatata tccgctgaat gcatagttat
    15481 tgccggtcag cgccactgtc ccactgtcga tacttattgt accgttgccg gaaatcttat
    15541 tggcaaaggt catgctatcg tcagccttca ggtgtaattt cccgcggttg ttgatttctg
    15601 cggtacctat attttgggtg atccctaaag cgccattttg ctcaattttg actttaccgc
    15661 taaaggcgga gttatccccg gttaagtaag tcgtaccatt gagcgcattg atatagccca
    15721 ggccctcgct gtcactgaca atatccgcgg aaatagtcgc gtttttatcc gtgtggttta
    15781 aggtgatttt cttctcggat gtctttgccc acacaaactc aattttttcg gcatcaataa
    15841 tccctgcagc cttcgctgcg cttccttcct gtgcacctaa cgctaactca gagttggtgc
    15901 ttaaactaat agtcggcgca gagattttac caccttccga aacagcaagt gttgtatcgg
    15961 agttttgatc ataataaccc aggctaattt tctcactggc attaacttca ccgccgttat
    16021 taactgacag gtactgatta ccaccgtaac tgccgtaaac gcccataaag aaggttttag
    16081 aatttacctg gctgccttca ccactgacat ttccctgcaa ataggtcaga cggctggtgc
    16141 ccttaacatg gattttgccg ccgttggaga cattaaaata gccgcgatcg tgtctcatgc
    16201 ctgagtttga ctcatacaaa tcatcagcat ataactcgct gccagcattg gagacattca
    16261 ggaaccccg
//
LOCUS       DAZPLI010000084        16265 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZPLI010000084 DAZPLI010000000
VERSION     DAZPLI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16265
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.085"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(95..1297)
                     /gene="ubiI"
                     /locus_tag="ACHUU3_002618"
                     /gene_synonym="visC"
     CDS             complement(95..1297)
                     /gene="ubiI"
                     /locus_tag="ACHUU3_002618"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR2082644.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(1320..2498)
                     /gene="ubiH"
                     /locus_tag="ACHUU3_002619"
                     /gene_synonym="visB"
     CDS             complement(1320..2498)
                     /gene="ubiH"
                     /locus_tag="ACHUU3_002619"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR2082645.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(2495..3820)
                     /gene="pepP"
                     /locus_tag="ACHUU3_002620"
     CDS             complement(2495..3820)
                     /gene="pepP"
                     /locus_tag="ACHUU3_002620"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR2082646.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNESEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(3846..4424)
                     /gene="ygfB"
                     /locus_tag="ACHUU3_002621"
     CDS             complement(3846..4424)
                     /gene="ygfB"
                     /locus_tag="ACHUU3_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR2082647.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            4592..4921
                     /gene="zapA"
                     /locus_tag="ACHUU3_002622"
     CDS             4592..4921
                     /gene="zapA"
                     /locus_tag="ACHUU3_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR2082648.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            4963..5146
                     /gene="ssrS"
                     /locus_tag="ACHUU3_002623"
     ncRNA           4963..5146
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHUU3_002623"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5167..5769
                     /locus_tag="ACHUU3_002624"
     CDS             5167..5769
                     /locus_tag="ACHUU3_002624"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR2082649.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(5870..5929)
                     /locus_tag="ACHUU3_002625"
     CDS             complement(5870..5929)
                     /locus_tag="ACHUU3_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR2082650.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(6261..6320)
                     /gene="ibsC"
                     /locus_tag="ACHUU3_002626"
     CDS             complement(6261..6320)
                     /gene="ibsC"
                     /locus_tag="ACHUU3_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR2082651.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(6549..7781)
                     /gene="serA"
                     /locus_tag="ACHUU3_002627"
     CDS             complement(6549..7781)
                     /gene="serA"
                     /locus_tag="ACHUU3_002627"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR2082652.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(8037..8696)
                     /gene="rpiA"
                     /locus_tag="ACHUU3_002628"
     CDS             complement(8037..8696)
                     /gene="rpiA"
                     /locus_tag="ACHUU3_002628"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR2082653.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            9126..10019
                     /gene="argP"
                     /locus_tag="ACHUU3_002629"
     CDS             9126..10019
                     /gene="argP"
                     /locus_tag="ACHUU3_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR2082654.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            10222..12366
                     /gene="scpA"
                     /locus_tag="ACHUU3_002630"
     CDS             10222..12366
                     /gene="scpA"
                     /locus_tag="ACHUU3_002630"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR2082655.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            12359..13354
                     /gene="meaB"
                     /locus_tag="ACHUU3_002631"
     CDS             12359..13354
                     /gene="meaB"
                     /locus_tag="ACHUU3_002631"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR2082656.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            13365..14150
                     /gene="scpB"
                     /locus_tag="ACHUU3_002632"
     CDS             13365..14150
                     /gene="scpB"
                     /locus_tag="ACHUU3_002632"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR2082657.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            14174..15652
                     /gene="scpC"
                     /locus_tag="ACHUU3_002633"
     CDS             14174..15652
                     /gene="scpC"
                     /locus_tag="ACHUU3_002633"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFR2082658.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15649..>16265)
                     /locus_tag="ACHUU3_002634"
     CDS             complement(15649..>16265)
                     /locus_tag="ACHUU3_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR2082659.1"
                     /translation="IVIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLF
                     DRQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQM
                     RFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNP
                     ATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         3950 a         4382 c         4139 g         3794 t
ORIGIN      
        1 aaattagttt atttcaaatg aggaaaatct cccggcgaaa aaaccgggag atgaaagtgt
       61 gatgggtatc aaataaacag cagaagagaa atttttaacg cagccattca ggcaaatcgt
      121 ttaatcccat tgcctggcgg ataagttgtg gtttaacgcc aggaagcgtg tcggccagtt
      181 tcaaaccaat atcacgcagc agttttttcg ccggattggt accggaaaac agatcgcgga
      241 atccctgcat accagccagc atcagcgccg cactgtgctt gcggctacgc tcataccggc
      301 gcaggtaaat gtactgcccg atgtctttcc cctgacgatg caaccgtttc agttcagcaa
      361 tcagctctgc agcatccata aagccaagat ttactccctg ccccgccagc gggtgaatgg
      421 tatgcgcggc gtcgcccacc agcaccagac ggtgcgcggc aaactggcgc gcataacgcc
      481 ccgtcagtgg gaacacctga cgcgcgctct caaccttgca taagcccagg cgattatcaa
      541 aagcgatatt taacgcgcga ttaaattcat cttcacttgc ctgctgcatc cgctgcgctt
      601 cctctggcga cagtgaccag acaattgagc aaagatgcgg atcgctaagc ggtaaaaagg
      661 ccagaatgcc ttcgccatgg aaaacctgcc gcgccaccgc atcatgcggt tcttccgtgc
      721 gaatggtcgc taccagcgcg tgatgctgat aatcccagaa agtcagcgga atatctgctt
      781 tgttgcgcaa ccaggaatta gcaccgtccg cgccaatcac cagacgcgcc gttaacatgc
      841 tgccatcttt cagcgtcagg aaagtttcat tttctcccca ggcgacctgc tgtaattctg
      901 cgggggccaa cagagtgata tctgacgact gctgcgcttt gttccacagc gcgtagtgaa
      961 tcactgagtt ttcaacgata tgcccaagat ggctatagcc catgctttga tcgtcaaacg
     1021 aaatgtgacc aaaactgtct ttgtcccaca cttccatacc gtgataacaa ctggccctac
     1081 gagagagaat gtcctgccag acgccaagac gggtgagtaa tttttcgctg gcggcattga
     1141 tagccgaaac gcgcagttgt ggtggtgcat ccgccgccag aggttccggt acgcgctgct
     1201 ccagtacggc aacgcgtaag ccgctcccct gtaagccgca ggcaaccgcc agccccacca
     1261 tgccgccacc aacaatggct acatcaacac tttgcattgt ttattcctta aaaccgcctt
     1321 caacgcgcca cccaaccgag ggtgcgctgc gccagcacat cgcgtgccgg ggtgaataat
     1381 tccatcgtca tcagtccgat gttgcgcccg acaaccagcg gtgcccaacg gttggcaaaa
     1441 agatgtacca ggctgtccgt gacgccgatg gttgcttcgc gatcgctctg tcgacgctgc
     1501 tgatagcggc acaatatgcc gtaatcaccg atgtcttctc cgcgctcatg cgcctgagtc
     1561 agggtttccg caaggctcat gacatctcgc ataccgaggt taaacccttg cccggcaatc
     1621 gggtgcagag tttgcgccgc attgcccacc agcacggtac gatgggtaat agctctggcg
     1681 gcgcgggtta acgccagcgg ataagcactg cgtttaccgg cgtgggtaat ttttccaagt
     1741 cgccagccaa aggccgactg gagttcacgg caaaacttct cgtcgctcca cgacagcacc
     1801 tcttcgcgcc gttccagtgg atgacaccag accagcgaac agcgtccgtc agacatcggc
     1861 aacatcgcca gcgggccatg ttgcgtgaag cgttcaaaag cgcggccttc gtgcgcaacg
     1921 gaagtcgcaa cgttggcaat cacggccagt tgttcgtaag gctcctgctg ccagtcaacg
     1981 ccgcacgcgg tggctaacgc tgaatgggtg ccatcagctg ctaccagcac gcggcccgtc
     2041 agcgtctcgc cactctccag cgtcacttca acgtgactct gagtacgggc aacgttagcc
     2101 acgcgatcag ggcaatgcag cgttacgcca ggtgctttac gcagcaatgc aaacagccgt
     2161 tgcccgacat tgtgcaattc gacaacctgt cccagcgccg ccagttggta atcttctgcg
     2221 gcgagggtga caaatccagc gtgaccacga tcgctgacat gcacggtagt gatggcggtt
     2281 gcgtaatccg ccagagattg ccagacgccg atgcgcgcca gttgctgaca ggtccccgca
     2341 gccagcgcaa tagctcgtcc atcaaagccc ggatgagcat gtgactctgg cgcggtcgct
     2401 tcaatcaaat gtaccggcag cgccccgtga cttaaccggg aaatagccag cgccagcgtc
     2461 gcgcccgcca tgccgccacc gacgatgatt acgctcattg ctttctcgca gctgccatca
     2521 acgcttcgat ttcttccggc tttttcacca cgctggcggt gaggttttcg ttgccggttt
     2581 cggtaatcac aatgtcgtct tcaatacgaa tgccgatacc gcgatattgt tctggcacat
     2641 ccgcatccgg cgcgatatac agaccaggct ctacggtcag taccatcccc ggttccagaa
     2701 tgcgcgagcg atcctgacca taaacgccaa cgtcatggac atccagtcct aaccagtggc
     2761 taaggccatg cataaagaaa ggacgatggg cgttctgagc gatcagttca tcaacatcac
     2821 ctttcaggat gccgagtttt accaggccgc taaccatgat gcgcaccact tcaccagtga
     2881 cttccagaat ggaagttccc ggacgataca ggcgcaggct ggtttcgaga gactccagca
     2941 caatgtcgta gatttcacgc tgggcctggg tgaatttgcc gttgaccggg aaggtgcggg
     3001 taatatcgcc cgcgtaacct ttgtattcac aacccgcgtc aatcaacacc aggtcaccgt
     3061 cgcgcagttc actctcgttt tcggtgtagt gcagaatgca gccgttttca ccgctgccga
     3121 caatggtgtt ataggacgga tagcgcgcac cgtggcggtt aaattcgtgg tgaatttcgc
     3181 cttccagatg gtactcgaac attcccggac ggcatttttc catcgcccgt gtatgtgcca
     3241 tggcggtgat ttctcccgcg cggcggagta cggcaatctc ttctggcgat ttgaacaggc
     3301 gcatttcatg aacaacagga cgccagtcga tcatcgttgc cggtgcggtg agattttgcc
     3361 gcgaaccttt acgcagtttt tccagcgcac tgttcatgat ttcatcagca tatgcatatt
     3421 cgccctgggc atggtaaacc acatccaggc cgttaagtag ttgataaagt tgctgattga
     3481 tttcgctgaa tgccagtgcg cggtcaacgc ccagtttctc tggcgcggca tcctggccta
     3541 agcgacggcc aaaccagatc tccgccgtca ggtcgcgaac gcggttaaac agaacgctgt
     3601 ggttatgagt gtcatcgctt ttaatcagca ccagcaccgc ttccgattcg ttaaagccgg
     3661 tgaagtacca gaagtcactg ttctgacgat aggggtattc gctgtcggcg ctacgtgtta
     3721 cttctggtgc agcaaaaatc agcgcggcgc tgccgggttg catttgctcc accagggcct
     3781 gacggcgacg ctgaaactct tgccgggata tctcactcat aacactctcc ttacgttttt
     3841 tgtttttagt gtagagtcgg tttttgtact tctggcgcgg tcggttgcgg atgagtaaag
     3901 gtgtcgtggc ataacagcgc ggcaacacga acgtattcga tgatctcttc aagcgacatt
     3961 tcaagctctt cctgatcttc gtcttcgtcg taacccagtt gcgcaatgtt acgcagatcg
     4021 tcgatagctt caccggtttc gccggtcact ttatccagct tcggttgcgt aacgccaaga
     4081 ccaagcagga agtgattgac ccaacctgcc aatgcatcag cccgatcgaa aacgctgaca
     4141 tcatcgccat caggcagata aagctgaaaa aggaagccgt catcctgcag ggcatcgctg
     4201 gtggcagagt gcattttacg cagtgcctgt gccagctcat gaccgaaagc catgccttcg
     4261 ttcgtcaggt cgtgaagtag cggcagccat gagctgtcat cgttaccgcc acatatcatc
     4321 ccactgatta aaccatgcat ctcggcaggg gtcagccccg tcccttgttg gttcagatac
     4381 tggttcattt cgttgtaacc aggcatttcg ttctgtatag acataagcat tcgtcgtcaa
     4441 agggaggaat attcatgata tgctaccact ttgggccctg gtggaccaga aaagggcttg
     4501 tctcttctca tcagggtagc tatagtgtcg ccccttcgca gaccatgggt ctaaagacga
     4561 aggcagcgca gtcaatcagc aggaaggtgg catgtctgca caacccgtcg atatccaaat
     4621 ttttggccgt tcactgcgtg tgaactgccc gcctgaccaa agggatgcgt tgaatcaggc
     4681 agcggacgat ctgaaccaac ggttgcaaga tctgaaagaa cgcactagag tcacaaatac
     4741 tgaacagttg gtcttcattg ccgcattgaa tatcagctat gagttagcgc aagaaaaagc
     4801 gaagactcgt gactacgcgg caagtatgga acagcgtatt cggatgctgc agcagaccat
     4861 agaacaagcg ttacttgaac aaggtcgcat caccgaaaaa actaaccaaa actttgaatg
     4921 acacttttcg gtttactgtg gtagagtaac cgtgaagaca aaatttctct gagatgttcg
     4981 caagcgggcc agtcccctga gccgatattt cataccacaa gaatgtggcg ctccgcggtt
     5041 ggtgagcatg ctcggtccgt ccgagaagcc ttaaaactgc gacgacacat tcaccttgaa
     5101 ccaagggttc aagggttaca gcctgcggcg gcatctcgga gattcccttc ttatctggca
     5161 ccagccatga cgcaactacc agaactccca ctgacattat cccgacaaga aatccgcaaa
     5221 atgattcggc aacgtcgtcg tgcgttaacg ccggaacaac agcaggaaat gggtcaacaa
     5281 gccgctaccc ggatgatgac ttatcccccg gtggtgatgg cgcatacggt cgctgtattc
     5341 ctctcttttg atggtgaact cgacacccag ccgctaatag aacaactctg gcgcgccggt
     5401 aagcgcgtat atcttccagt tttgcatccc tttagtgccg gtaatttgct gttcctgaat
     5461 taccatccgc aaagcgaact ggtgatgaac aggttgaaga tccatgagcc aaaactggat
     5521 gtgcgtgacg tgctacccct ttcccgatta gacgtgctga tcacaccgct ggtcgccttt
     5581 gatgagtacg gtcagcgcct gggaatgggc ggtggttttt atgatcggac cttacaaaac
     5641 tggcagcact ataaaacgca accggtgggt tatgcgcatg attgtcagtt ggtggaaaaa
     5701 ctccccgttg aagagtggga tatccctctt cctgcggtgg ttacaccgtc gaaagtctgg
     5761 gagtggtaag ggcgatacac ctgcatcgcc ctgattgaca tcgttgattc tttaacctaa
     5821 tttagtgagt aagggtaagg gaggattgct cctcccctgt gactgactgt taataagctg
     5881 ggaaacttat gagtaacagt acaatcagta tgatggcaag tcgcatcata acccttctcc
     5941 ttcaagccct cgcttcggtg agggctttac cgttacagcc ccatgctgcc cagctatcgt
     6001 aagctctctt taaataacca caacgcattg atctaccttt aaatcttttc ttcgagcgtg
     6061 ctcgaaaaac ccatgcaaaa cagagataat ttttgggaaa aaggatctat tacggcagta
     6121 aacgtgggcg atgaagtggc tatcaaaaag ttcggttcag ggcgatgcac cccgcatcgc
     6181 cctgattgac atcgttgatt ctttgaccta atttagtgag taagggtaag agaggatgac
     6241 tcctcccctg agactgactg ttaataagct ccgaaactta tcagtaacag tacaatcagt
     6301 atgatgacaa gtcgcatcat aacccttctc ctttaagccc tcgcttcggt gagggcttta
     6361 ccgttacagc cccatgctgt cccgccatcg taaatcccca ttaaataaac acaacgcatt
     6421 gatctgagtt tgatttattt tctggagcag actcgcaaag tagaatgcgc aacgcggcaa
     6481 cggtgtggag aagggataaa aaaacgggca agtcagtgac ctgcccgtta attttcagag
     6541 aaggggaatt agtacagcag acgggcgcga atggtacccg gaatagcttt cattgcctgc
     6601 agcgcttttt cggcaacgtc ttcgtcagct tcaatatcaa taactacata acccatctgg
     6661 gcggaagttt gtagatattg cgcggcgatg ttgacgccct gctcggcgaa gattttgttc
     6721 agcgcagtta gcacgcccgg acggttttcg tggatgtgca tcagacgacg accaccatgc
     6781 agtggcagcg agacttccgg gaagttcacc gcagagagcg ttgagccatt gtcagaatac
     6841 ttgatcaatt tacccgcaac ttccaggccg atattctcct gcgcttcctg agtcgaaccg
     6901 ccaatgtgtg gcgtcagaag gacgttgtcg aattcacaca gtggagaggt aaatggatcg
     6961 ctattggtcg ccggttccgt cgggaatacg tcgattgccg cccccgccag atgtttgctc
     7021 gccagcgcat cacacagcgc cggaatatcc accacagtac cgcgcgaggc attaatcagc
     7081 agcgagccgg gcttcattag cgaaatctct ttcgcgccca tcatattttt ggtggacgga
     7141 ttctctggta catgcagact caccacatcg ctcatattca gcaggtcaga aagatgctgt
     7201 acctgagtgg cgttgcccag cggcagtttg ttttcaatat cataaaagta aacatacatt
     7261 cccagcgatt cagccagaat gcccaattgc gtaccaatat gaccgtagcc gatgataccc
     7321 agctttttgc cgcgcgcttc aaaagaaccc gccgccagtt tgttccacac gccacggtgc
     7381 gctttagcat tggcttccgg cacgccgcgc aatagcagca gcagttcgcc aatcaccagc
     7441 tccgcaacag agcgcgtatt tgagaacggt gcgttaaata ccgggatccc gcgctttgcc
     7501 gccgcatcca gatcaacctg gtttgttccg atacagaaac agccaatagc gaccagtttt
     7561 tctgcggcgt tgatcacgtc ttcagtcaga tgggtacggg atcgcaggcc gatgaagtgg
     7621 gcatcgcgga tggattcttt taattgttca tcatccagcg cgcctttgtg aaattcgatg
     7681 ttggtgtaac cagctgcacg aaggctttcc agcgcctttt ggtgcacgcc ttctaccagc
     7741 agaaacttaa tcttgtcttt ctccagcgat acctttgcca tttacccaat cctgtctttt
     7801 gaaatgttgt gtgcggattt gcatccgcct ttcaacatat caaaaaataa tattgcggca
     7861 atatgaacgt ttgcgtcgcg atgttgagga aatatcacgc aaaggtattt cttggttgaa
     7921 tatgctggtt tagaggattt atttagagca atcgacaata gccaagtaaa aacgtgacac
     7981 atgtcaccaa atttaatgaa gagaattttt ttaacggggg aggttccccc gtcagatcat
     8041 ttcacaatgg ttttgacacc gtcaggtgtg ccaatcagcg caacgtccgc gccacggtta
     8101 gcaaacaagc caacagtcac cacgccagga atcgcattta tggcgttttc catcgctatc
     8161 gggtcaagga tttccatgcc gtggacgtcg aggatcacgt tgccgttatc ggtcaccacg
     8221 ccctgacggt attccggacg accgcccagt ttcaccagct ggcgcgccac tgcactacgt
     8281 gccatcggga taacttctac tggcagcggg aatttgccca gaatatcaac ctgcttggaa
     8341 gcgtcagcaa tacagataaa tttttccgca accgaagcaa tgattttttc acgggtcagc
     8401 gccgcgccgc cgcctttgat catttgcatg tggccgttga tttcatctgc gccatcaacg
     8461 tagatgccaa ggctgtcgac ttcgttgaga tcaaaaacgt gaatgccgag gcttttcagt
     8521 ttttcagtgg aagcatctga actggaaacg gccccttcaa tctggccttt cattgtaccg
     8581 agcgcgtcaa taaagtgtgc ggcggtggaa cctgtaccta caccaacaat ggtgccgggc
     8641 tgaacatact gaagtgccgc ccatcctact gcttttttca attcatcctg cgtcatgatc
     8701 gtttcgcctg tggtatgaaa tttcacacgc attatataca aaaaaagcga ttcagacccc
     8761 gttggcaagc cgcgtggtta actcatccat aaaatatcgc gcaatggcgg gcatcccctt
     8821 tcgccccgca aataaagcat acaacggtct gggtatgccg ctccacggtg cgaacaggcg
     8881 caccagttcc ccccgttcgc aagcccctgt ttacaggcaa attgaggcaa taacgccacc
     8941 ccattcaaca caaccagggc acgaactcgc tgagcgaaat gcattagcgc aaatccctga
     9001 tcttttaagc tgcgcgttta accacggata caaatgttac ccgccgacgg cttcggtata
     9061 tgcaacctga cacaaaattg tgtcatagtg caggaaaaag catttaccag gagcagacaa
     9121 cagcaatgaa acgcccggac tacagaacat tacaggcact ggatgcggtg atacgtgaac
     9181 gaggatttga gcgcgcggca caaaagctgt gcattacaca atcagccgtc tcacagcgca
     9241 ttaagcaact ggaaaatatg ttcgggcagc cgctgttggt gcgtaccgta ccgccgcgcc
     9301 cgacggaaca agggcaaaaa ctgctggcac tgctgcgcca ggtggagttg ctggaagaag
     9361 agtggctggg cgatgaacaa accggttcga ctccgctgct gctttcactg gcggtcaacg
     9421 ccgacagtct ggcgacgtgg ttgcttccgg cactggctcc tgtgttggct gattcgccta
     9481 tccgcctcaa cttgcaggta gaagatgaaa cccgtactca ggaacgtctg cgccgcggcg
     9541 aagtggtcgg cgcggtgagt attcaacatc aggcgctgcc gagttgtctt gtcgataaac
     9601 ttggtgcgct cgactatctg ttcgtcagct caaaaccctt tgccgaaaaa tatttcccta
     9661 acggcgtaac gcgttcggca ttactgaaag cacctgtggt cgcctttgac catcttgacg
     9721 atatgcacca ggcctttttg cagcaaaact tcgatctgcc tccaggcagc gtgccctgcc
     9781 atatcgttaa ttcttcagaa gcgttcgtgc aacttgctcg ccagggcacc acctgctgta
     9841 tgatcccgca cctgcaaatc gagaaagaac tagccagcgg tgaactgatt gacttaacgc
     9901 cagggctatt tcaacgccgg atgctctact ggcaccgctt tgctcctgaa agccgcatga
     9961 tgcgtaaagt cactgatgcg ttgctcgatt atggtcacaa agtccttcgt caggattaat
    10021 ccatcaaata atgcctgata gcacatatca ggcgttatcc tcacttcttt ttgtattcct
    10081 tgaatcacat cacaaaatag acaaatctca ggcggcaaaa aacgacgtct gaatgcattt
    10141 ttttgctggc gacaaaccca cgtaaaaagc tcaccgtaag cgcaaatacc ctcattttga
    10201 ttgcgtttta cggagcaaat aatgtctaac gagcaggagt ggcaacagct tgccaacaag
    10261 gaattgagcc gtcgggagaa aactgtcgac tcgctggttc agcaaaccgc ggaagggatc
    10321 gccatcaagc agctgtatac cgaagccgat ctcgataatc tggaggtgac aggtaccctt
    10381 cctggtttgc cgccctacgt tcgtggcccg cgtgccacta tgtataccgc ccaaccgtgg
    10441 accatccgtc agtatgctgg tttttcaaca gcaaaagagt ccaacgcttt ttatcgccgt
    10501 aacctggccg ccgggcaaaa aggtctttcc gttgcgtttg accttgccac ccaccgtggc
    10561 tacgactccg ataacccacg cgtggcgggt gacgtcggca aagcgggcgt cgctatcgac
    10621 accgtggaag atatgaaagt cctgttcgac cagatcccgc tggataaaat gtcggtttcg
    10681 atgaccatga atggcgcagt gctaccagta ctggcgtttt atatcgtcgc cgcagaagag
    10741 caaggtgtta cacctgataa actgaccggc accattcaaa acgatattct caaagagtac
    10801 ctctgccgca acacctatat ttacccacca aaaccgtcaa tgcgcattat cgccgacatc
    10861 atcgcctggt gttccggcaa catgccgcga tttaatacca tcagtatcag cggttaccac
    10921 atgggggaag cgggtgccaa ctgcgtgcag caggtagcat ttacgctcgc cgatgggatt
    10981 gagtacatca aagcagcaat ctctgccgga ctgaaaattg atgacttcgc tcctcgcctg
    11041 tcgttcttct tcggtatcgg catggatctg tttatgaacg tcgccatgtt gcgtgcggca
    11101 cgttatttat ggagcgaagc ggtcagtgga tttggcgcac aggatccgaa atcactggcg
    11161 ctgcgtaccc actgccagac ctcaggctgg agcctgactg aacaggatcc gtataacaac
    11221 gttatccgca ccaccattga agcactggct gcgacgctgg gcggtactca gtcactgcat
    11281 accaacgcct ttgacgaagc gcttggtttg cctaccgatt tctcagcacg cattgcccgc
    11341 aacacccaga tcatcatcca ggaagaatca gaactctgcc gcaccgtcga tccactggcc
    11401 ggatcctatt acattgagtc gctgaccgat caaatcgtca aacaagccag agctattatc
    11461 caacagatcg acgaagccgg tggcatggcg aaagcgatcg aagcaggtct gccaaaacga
    11521 atgatcgaag aggcctcagc gcgcgaacag tcgctgatcg accaaggcaa gcgtgtcatc
    11581 gttggtgtca acaagtacaa actggatcac gaagacgaaa ccgatgtact tgagatcgac
    11641 aacgtgatgg tgcgtaacga gcaaattgct tcgctggaac gcattcgcgc cacccgtgat
    11701 gatgccgccg taaccgccgc gttgaacgcc ctgactcacg ccgcacagca taacgaaaac
    11761 ctgctggctg ccgctgttaa tgccgctcgc gttcgcgcca ccctgggtga aatttccgat
    11821 gcgctggaag tcgctttcga ccgttatctg gtgccaagcc agtgtgttac cggcgtgatt
    11881 gcgcaaagct atcatcagtc tgagaaatcg gcctccgagt tcgatgccat tgttgcgcaa
    11941 acggagcagt tccttgccga caatggtcgt cgcccgcgca ttctgatcgc taagatgggc
    12001 caggatggac acgatcgcgg cgcgaaagtg atcgccagcg cctattccga tctcggtttc
    12061 gacgtagatt taagcccgat gttctctaca cctgaagaga tcgcccgcct ggccgtagaa
    12121 aacgacgttc acgtagtggg cgcatcctca ctggctgccg atcataaaac gctgatcccg
    12181 gaactggtcg aagcgctgaa aaaatgggga cgcgaagata tctgcgtggt cgcgggtggc
    12241 gtcattccgc cgcaggatta cgccttcctg caagagcgcg gcgtggcggc gatttatggt
    12301 ccaggtacac ctatgctcga cagtgtgcgc gacgtactga atctgataag ccagcatcat
    12361 gattaatgaa gccacgctgg cagaaagtat tcgccgctta cgtcagggtg agcgtgccac
    12421 gctcgcccag gccatgacgc tggtggaaag ccgtcaccca cgtcatcagg cgctaagtac
    12481 acagctgctt gacgccatta tgccgttctg tggtaacgcc ctgcgactgg gcgttaccgg
    12541 cacacccggc gcggggaaaa gtacctttct tgaagccttt ggcatgttgt tgattcgtga
    12601 gggattaaag gttgctgtta ttgcggtcga tcccagcagc ccggtcactg gcggtagcat
    12661 tctcggggat aaaacccgca tgaatgacct ggcgcgtgcc gaggcggcgt ttattcgccc
    12721 ggtaccctcc tccggtcatc tgggcggtgc cagtcagcga gcacgggaat taatgctgtt
    12781 atgcgaagcg gcgggttatg acgtagtgat tgtcgaaacg gttggcgtcg ggcagtcgga
    12841 aacggaagtc gcccgcatgg tggactgttt tatctcgttg caaattgccg gtggcggcga
    12901 tgatctgcag ggcattaaaa aagggctgat ggaagtggct gatctgctcg ttatcaacaa
    12961 agacgatggc gataaccata ccaatgtcgc cattgcccgg catatgtacg agagtgccct
    13021 gcatattctg cgacgtaaat acgacgaatg gcagccacgg gttctgactt gtagcgcact
    13081 ggaaaaacgt ggaatcgatg agatctggca cgccatcatc gacttcaaaa ccgcgctaac
    13141 tgccagtggt cgtttacaac aagtgcggca acaacaatcg gtggaatggc tgcgtaagca
    13201 gaccgaagaa gaagtactga atcacctgtt cgcgaatgaa gatttcgatc gctattaccg
    13261 ccagacgctt ttagcggtca aaaacaatac gctctcaccg cgcaccggcc tgcggcagct
    13321 cagtgaattt atccagacgc aatattttga ttaaaggaat ttttatgtct tatcagtatg
    13381 tgaacgttgc cactatcaac aaagtggcgg tcattgagtt taactatgga cgaaaactta
    13441 atgccttaag taaagtcttt attgatgatc ttatgcaggc gttaagcgat ctcaaccggc
    13501 cggaaattcg ctgtatcatt ttgcgggcgc caagtggatc caaagtcttc tccgcaggtc
    13561 acgatattca cgaactgccg tctggcggtc gcgatccgct ctcctatgat gatccattgc
    13621 gtcaaatcac ccgcatgatc caaaaattcc cgaaaccgat catttcgatg gtggaaggta
    13681 gtgtttgggg tggcgcattt gaaatgatca tgagttccga tctgatcatc gccgccagta
    13741 cctcaacctt ctcaatgatg cctgtaaacc tcggcgtccc gtataacctg gtcggcattc
    13801 acaacctgac ccgcgacgcg ggcttccaca ttgtcaaaga gctgattttt accgcttcgc
    13861 caatcaccgc ccagcgcgcg ttggctgtcg gcatcctcaa ccatgttgtg gaagtggaag
    13921 aactggaaga tttcacctta caaatggcgc accacatctc tgagaaagcg ccgttagcca
    13981 ttgctgttat caaagaagag ctgcgtgtac tgggcgaagc gcacaccatg aactccgatg
    14041 aatttgaacg tattcagggg atgcgccgcg cggtttatga cagcgaagat taccaggaag
    14101 ggatgaacgc tttcctcgaa aaacgtaaac ctaatttcgt tggtcattaa tccctgcgaa
    14161 cgaaggagta aaaatggaaa ctcagtggac aaggatgacc gccgatgaag cggcagaaat
    14221 tatccagcat aacgacatgg tggcatttag cggctttacc ccagcgggtt cgccgaaagc
    14281 cttaccgacc gcgattgccc gcagagctaa cgaacagcat gaggccaaaa agccgtatca
    14341 aattcgcctg ctgacggggg cgtcaatcag cgccgccgct gacgatgtac tttctgacgc
    14401 cgatgctgtt tcctggcgtg cgccatatca aacatcgtcc ggtttacgta aaaagatcaa
    14461 tcagggcgcg gtgagtttcg ttgacctgca tttgagcgaa gtggcgcaaa tggtcaatta
    14521 cggtttcttc ggcgacattg atgttgccgt catcgaagca tcggcactgg caccggatgg
    14581 tcgagtctgg ttaaccagcg ggatcggtaa tgcgccgaca tggctgctgc gggcgaagaa
    14641 agtgatcatt gaacttaatc actatcacga tccgcgcgtt gcagaactgg cggatattgt
    14701 gattcctggc gcaccaccgc ggcgcaatag cgtgtcgatc ttccatgcaa tggatcgcgt
    14761 cggtacccgc tatgtgcaaa tcgatccgaa aaagattgtc gccgtcgtgg aaaccaactt
    14821 gcccgacgcc ggtaatatgc tggataagca aaatcccatg tgccagcaga ttgccgataa
    14881 cgtggtcacg ttcttattgc aggaaatggc gcatgggcgt attccgccgg aatttctgcc
    14941 gctgcaaagt ggcgtgggca atatcaataa tgcggtaatg gcgcgtctgg gggaaaaccc
    15001 ggaaattcct ccgttcatga tgtattcgga agtgctacag gaatcggtgg tgcatttact
    15061 ggaaaccggc aaaatcagcg gggccagcgc ctccagcctg acaatctcgg ccgattccct
    15121 gcgcaagatt tacgacaata tggattactt tgccagccgc attgtgttgc gtccgcagga
    15181 gatttccaat aacccggaaa tcatccgtcg tctgggcgtc atcgctctga acgtcggcct
    15241 ggagtttgat atttacgggc atgcaaattc aacgcacgta gccggggtcg atctgatgaa
    15301 cggcatcggc ggcagcggtg attttgaacg caacgcgtat ctgtcgatct ttatggctcc
    15361 gtcgattgct aaagaaggca agatctcaac cgtcgtgcca atgtgcagcc atgttgatca
    15421 cagcgaacac agcgtcaaag tgatcatcac cgaacaaggg atcgccgatc tacgcggtct
    15481 ttccccgctt caacgcgccc gcactatcat tgataattgt gcacatccta tgtatcggga
    15541 ttatctgcat cgctatctgg aaaatgcgcc cggcggacat attcaccacg atcttagcca
    15601 cgtcttcgac ttacaccgta atttaattgc aaccggctcg atgctgggtt aatggtttgt
    15661 cccgtcacga tcttcagcaa tatgctgaag catcgtggcg gtataacgat gatttctgat
    15721 tgaataaaga tattcctgca tatacatcgg cctgtgcggc gcatcaaaat atttcagcgt
    15781 tgccggattc accagtcgcc aggcaaaatg cgggatcacc gtcagaaaat gtccgcgctc
    15841 cacggcgctg attttggcca taaaactgta gggacgataa ataatggtgg gattgatccc
    15901 acaaggtcgc atattcgcat caatcattgc ttcgaagtta gcccggtttt gaaagcgcat
    15961 ttgtagccag ggcagctcct gaagcagctc ttgctcttga cgctcttcat aacggcgaga
    16021 aacgagaaag cccaatcgca gcggtggtaa ttcactgata gtcaggtttt ccagttcctg
    16081 aactcgtgca gatacatgct gtggggagat aataaaatct aactgacggt caaaaagatt
    16141 atcgataacg ccgttttcgc tgaactcaac tggctgggcg gtcacgcctt catacttatc
    16201 tccgagacta atcaactgat cgaaaataat tgtcggataa gtattatcta tgccaatcac
    16261 aattt
//
LOCUS       DAZPLI010000085        16252 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZPLI010000085 DAZPLI010000000
VERSION     DAZPLI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16252
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.086"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..577
                     /locus_tag="ACHUU3_002635"
     CDS             <1..577
                     /locus_tag="ACHUU3_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR2082660.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENCYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            580..1743
                     /locus_tag="ACHUU3_002636"
     CDS             580..1743
                     /locus_tag="ACHUU3_002636"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR2082661.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1827..3452
                     /locus_tag="ACHUU3_002637"
     CDS             1827..3452
                     /locus_tag="ACHUU3_002637"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR2082662.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3569..3844)
                     /gene="yjfN"
                     /locus_tag="ACHUU3_002638"
     CDS             complement(3569..3844)
                     /gene="yjfN"
                     /locus_tag="ACHUU3_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR2082663.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3993..4322)
                     /gene="bsmA"
                     /locus_tag="ACHUU3_002639"
     CDS             complement(3993..4322)
                     /gene="bsmA"
                     /locus_tag="ACHUU3_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR2082664.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4504..5253
                     /gene="yjfP"
                     /locus_tag="ACHUU3_002640"
     CDS             4504..5253
                     /gene="yjfP"
                     /locus_tag="ACHUU3_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR2082665.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5250..6005)
                     /gene="ulaR"
                     /locus_tag="ACHUU3_002641"
     CDS             complement(5250..6005)
                     /gene="ulaR"
                     /locus_tag="ACHUU3_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR2082666.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6113..7177)
                     /gene="ulaG"
                     /locus_tag="ACHUU3_002642"
     CDS             complement(6113..7177)
                     /gene="ulaG"
                     /locus_tag="ACHUU3_002642"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR2082667.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7532..8930
                     /gene="ulaA"
                     /locus_tag="ACHUU3_002643"
                     /pseudo
     CDS             7532..8930
                     /gene="ulaA"
                     /locus_tag="ACHUU3_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            8946..9251
                     /gene="ulaB"
                     /locus_tag="ACHUU3_002644"
     CDS             8946..9251
                     /gene="ulaB"
                     /locus_tag="ACHUU3_002644"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR2082668.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9261..9722
                     /gene="ulaC"
                     /locus_tag="ACHUU3_002645"
     CDS             9261..9722
                     /gene="ulaC"
                     /locus_tag="ACHUU3_002645"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR2082669.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVADVVEPRYYQ
                     AILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDILI
                     TMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9736..10386
                     /gene="ulaD"
                     /locus_tag="ACHUU3_002646"
     CDS             9736..10386
                     /gene="ulaD"
                     /locus_tag="ACHUU3_002646"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR2082670.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10396..11250
                     /gene="ulaE"
                     /locus_tag="ACHUU3_002647"
     CDS             10396..11250
                     /gene="ulaE"
                     /locus_tag="ACHUU3_002647"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR2082671.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            11250..11936
                     /gene="ulaF"
                     /locus_tag="ACHUU3_002648"
     CDS             11250..11936
                     /gene="ulaF"
                     /locus_tag="ACHUU3_002648"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR2082672.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            12033..12584
                     /locus_tag="ACHUU3_002649"
     CDS             12033..12584
                     /locus_tag="ACHUU3_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFR2082673.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12659..12934)
                     /gene="yjfY"
                     /locus_tag="ACHUU3_002650"
     CDS             complement(12659..12934)
                     /gene="yjfY"
                     /locus_tag="ACHUU3_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFR2082674.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            13261..13656
                     /gene="rpsF"
                     /locus_tag="ACHUU3_002651"
     CDS             13261..13656
                     /gene="rpsF"
                     /locus_tag="ACHUU3_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR2082675.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13663..13977
                     /gene="priB"
                     /locus_tag="ACHUU3_002652"
     CDS             13663..13977
                     /gene="priB"
                     /locus_tag="ACHUU3_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR2082676.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13982..14209
                     /gene="rpsR"
                     /locus_tag="ACHUU3_002653"
     CDS             13982..14209
                     /gene="rpsR"
                     /locus_tag="ACHUU3_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR2082677.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14251..14700
                     /gene="rplI"
                     /locus_tag="ACHUU3_002654"
     CDS             14251..14700
                     /gene="rplI"
                     /locus_tag="ACHUU3_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR2082678.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14812..15783)
                     /locus_tag="ACHUU3_002655"
     CDS             complement(14812..15783)
                     /locus_tag="ACHUU3_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR2082679.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(15836..>16252)
                     /locus_tag="ACHUU3_002656"
     CDS             complement(15836..>16252)
                     /locus_tag="ACHUU3_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFR2082680.1"
                     /translation="SWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDM
                     TPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPIN
                     KIIKPMLPFYAVMVLVLAMVTYIPQISMALPKALGY"
BASE COUNT         3818 a         3968 c         4323 g         4143 t
ORIGIN      
        1 tggtagaccg aataatcctt ttgaaccgcg tcgcaatcgc tacgcacaaa aatacttaac
       61 gttggcgcta atgggcggtg ccgctttttt cgtattgaaa ggttgtggcg atagtagcga
      121 tgtcgataac gacggcgacg gaacgtttta cgcgacagtg caggattgta ttgatgacgg
      181 taataatgcc gacatttgcg cgcgtggctg gaacaacgcc aaaacggcat tttatgccga
      241 tgttccgaag aatatgactc agcagaactg tcagtctaag tacgaaaatt gctactacga
      301 caatgttgaa cagagttgga tcccggtcgt ttctggattt ttgttaagcc gggttattcg
      361 taaagatcgc gatgagccgt tcgtctataa cagcggcgga tcctcatttg cttcccgccc
      421 cgtctggcgc aacacttccg gtgattactc ctggcgctcc ggttctggca aaaaagagtc
      481 ttactcttca ggcggcttta ccaccaaaaa agcgtcaacc gtttcgcgcg gcggctatgg
      541 tcgttcttcc agcgcccgtg ggcattgggg aggctaatca tgctgagaca caacgttcct
      601 gtgcggcggg atctggacca aattgccgct gacaacggtt tcgactttca tatcatcgac
      661 aatgaaatct attgggacga gagtcgggct taccgtttta ctctgcgcca gattgaagag
      721 cagatcgaaa aaccaactgc ggaactgcat cagatgtgcc ttgaggtggt ggatcgcgcg
      781 gttaaagacg aagagatcct gacgcaactg gcgatcccgc cgttgtactg ggatgtgatc
      841 gccgaaagct ggcacgccag cgatccttcg ctgtatggcc ggatggattt tgcctggtgt
      901 ggcaatgcgc cggtgaagct gctggagtac aacgccgata cgccgacttc gttgtatgag
      961 tcggcttatt tccagtggtt gtggctggag gatgcccggc gcagcggcat tattccgcgt
     1021 gatgccgatc agtacaatgc cattcaggaa cgcctgattt cacgctttag cgagctttat
     1081 agccgggagc cgttttattt ctgctgctgt caggacaccg atgaagacag gactaccgtg
     1141 ctgtacttgc aggactgcgc ccagcaggca gggcaggagt cgcggtttat ctacattgaa
     1201 gatctcggtt tgggcgtcgg cggtgtactg accgatcttg atgataatgt catccagcgt
     1261 gcatttaagc tgtatccgct ggagtggatg atgcgtgacg ataacggtcc gctgctgtgc
     1321 aagcgtcgcg agcaatgggt ggagccgtta tggaaaagta tcttgagtaa taaagggcta
     1381 atgccgctgc tttggcgctt cttccctggt catcctaacc ttcttgcatc ctggtttgag
     1441 ggtgaaaaat cgcagattgc cgctggcgaa agctatgtgc gtaaaccgat ctactcgcgc
     1501 gaaggcggaa acgtcaccat tttcgacggt cagaataacg tcgttgacca cgctgatggt
     1561 gattacgccg atgaaccgat gatctaccag gcgtttcaac ctctgccgcg atttggcgat
     1621 agctacacac tcatcgatag ctggattgtc gatgatgaag cgtgcggaat ggggatccgt
     1681 gaagataaca cactgatcac caaagacacc tcacgtttcg ttccgcatta cattgctgga
     1741 taagaatgtt ttagcaatct ctttctgtca tgaatccatg gcagtggcca tactaatggt
     1801 gactgccatt gatggaggga gacacagtgc actggcaaac tcacaccgtt tttaatcaac
     1861 ctataccatt aaataacagc aacttatacc tgtctgatgg cgcgctttgc gaagcggtaa
     1921 cgcgtgaagg tgctggctgg gatagcgatt ttctagccag tattggtcag cagttaggaa
     1981 cggctgaatc ccttgaactg gggcggctgg cgaatgtaaa cccgcctgaa ctgttgcgct
     2041 acgatgccca ggggcggcgt ctggacgatg ttcgtttcca tcccgcatgg cacctgctga
     2101 tgcaggtgtt atgtaccaat cgggtgcaca atcttgcctg ggaagaagac gctcgctccg
     2161 gcgcatttgt ggcgcgcgcg gcgcgtttta tgttacatgc gcaggttgag gctgggtcgt
     2221 tatgtccaat aacgatgacc tttgccgcca cgccattgtt gttacagatg ttacccacgc
     2281 cgtttcagga ctggaccacg ccgctgctga gcgatcgcta cgattctcac ttattgccag
     2341 gtgggcaaaa acgcggtttg ttgattggca tgggaatgac ggaaaagcag ggtggttccg
     2401 atgtcatgag caacaccacc cgtgcagagc gtttggaaga tggctcttat cggctggtgg
     2461 ggcataaatg gtttttctcg gttccgcaaa gcgatgcgca tctggtgctg gcgcagacca
     2521 cgggcggtct gtcctgtttt tttgtgccgc gctttttgcc tgacgggcaa cgcaacgcga
     2581 ttcgcctcga acggctgaaa gataagctgg gtaatcgctc taacgccagt tgtgaagtgg
     2641 agtttcagga tgccattggc tggttattgg ggcaggaagg ggaaggaatt cgtctgatcc
     2701 tgaaaatggg ggggatgacg cgttttgatt gcgccctggg tagccatgcc atgatgcgcc
     2761 gtgcgttttc gctggcgatt tatcatgcac atcaacgcca tgtttttggt aatccattga
     2821 tccaacagcc ccttatgcgt catgtcttaa gtcgcatggc acttcagctt gaagggcaaa
     2881 cggcgttgct gtttcgtctt gcgcgagcgt gggaccggcg cgccgatgcc aaagaagccc
     2941 tgtgggcgcg tttatttacg cctgcggcaa aatttgtgat ctgcaaacga ggtatgccgt
     3001 ttgtggccga agcgatggag gtgctgggcg gcattggtta ttgcgaggag agcgagctgc
     3061 cgcggcttta ccgggagatg ccggtaaaca gtatttggga aggttccggc aatattatgt
     3121 gcctggatgt gttgcgcgtt ctcaataagc aagcgggcgt atacgactta ttgtcggaag
     3181 catttgtgga agtgaaaggg caggatcgct attttgatcg cgcggttcgt cgtttacagc
     3241 agcagctgcg taagccagct gaagaactgg ggcgagagat tactcatcag ctattcctgc
     3301 tgggctgcgg tgcgcaaatg ttgaaatatg cttctccgcc aatggcgcag gcgtggtgtc
     3361 aggtgatgtt agatacgcgc ggcggcgtac ggttgtcaga gcagatccag aatgatttat
     3421 tgctgcgggc gacgggggga gtgtgtttgt aaacgtatac gactgatgcg acgctggttt
     3481 cgattaactc aatgaaatat gtgaaaattg taggccggac aaggcgctcg cgccgcatcc
     3541 ggcattgttc atttgctgta gatgacactc atgcatacag tatcgcctgt acgcgccagt
     3601 tatcaggctg ctggtcttcg tacatggtaa taatgcggta ccatgacgca ccttgttcat
     3661 cggcctttaa cgccacgata cgttccacgt cctgcggatc ccctgaaata ttgctaactg
     3721 agatctgacc tatttcattc aggcccgtca cgcagtcagc actggcgaat tctgcagact
     3781 gtgccgtggc gctcgcagga taagtcacta attgcagcga gggtgaggca agtaattgtt
     3841 tcatcgtcag ctccattttt cgtttcccca catgttgcag ggcattccat cgcggtaata
     3901 aacgcccagg cttccagccg gtaatttatt gtgcacaggt aaacgcatag ggacgcaaag
     3961 caatgtaaag ctgactatgt aagttgtctg atttatttac ggtacaaaat ggcttgtgaa
     4021 taccactgtc ctgtcacgat ggtttcgtcc accatgacga caacgtaata atcagctttt
     4081 gcagcgacag cttttgctct gatttctgct aatgcgtcat ccggagaacc ccgaaccatc
     4141 gtgcttacgc tacctattcg ctgtaaccct tgcgtctggt cgcggcgaat ctcttgcgga
     4201 tggtccgtta ctggcggtgc tggctgtggc gtaccttgca gtgcgctaca ggcacttaac
     4261 atcaacacca ataataaact ggcaaaccgg taaataacgc tattacgttt cctgctaacc
     4321 atagtgtagt gccttattaa tttaactttg ggggaatgtt cccgaattgt tacctggtac
     4381 gcgattttcg aatgaaaacg tgacgaaagc gtaacccaca tctcaacatt atgaactaat
     4441 accgccagca gaaacttgtg ctaaggattt cttcatgtca taccagacaa aaaggagaga
     4501 cagatgattg aaatagaatc acgcgagctg gcagatattc ccgttcttca tgcttatcct
     4561 gtcgggcaaa aagatacccc gttaccgtgc gtaatttttt atcacggctt tacttcatcc
     4621 agcctggtgt atagctattt tgccgttgcg ctggcgcagg ctggtttgcg ggtgatcatg
     4681 ccggatgcgc ccgatcacgg tagccgtttt agtggcgacg aagcgcggcg gttaaatcaa
     4741 ttctggcaaa tcttgctaca aagtatgcag gaattcacta ctttacgtgc ggcaatagcc
     4801 gaagaaaact ggctgcttga tgaccgtctg gcagtcggtg gcgcgtcgat gggcgcgatg
     4861 acggcactgg gaattaccgc tcgccacccc acggtgaaat gtaccgccag catgatggga
     4921 tcgggctatt ttacatcgct cgcccgttca ctgtttccac cgctgatacc tgaaacggca
     4981 gcacagcaga atgaattcaa taacattatc gcgccgctgg cagagtggga agcgacaaac
     5041 cacctggaac aacttgggga cagacctcta ctgctgtggc atggcctcga cgatgacgtt
     5101 gtgcctgccg acgaatcact acgtttgcag caggccttaa gcgagacggg acgggataaa
     5161 ctgctaacct gttcatggca gccaggcgtg cgtcaccgca ttacgcctga ggcgttagat
     5221 gctgccgtga catttttccg ccagcatctt taaacacgca gaatgctgac cccttgcgct
     5281 tccagttgtt gcaggatttc cgggttagca tttttgccgg tgataagcat atcgatttga
     5341 tcggcacggc taaaaagcat tcccgcgcgt tcgccaatct tactgctatc aaccagtacc
     5401 accagtttcc ctaccacgct cagcatcttc tgctctgcca ttgctgtcag catatcggtt
     5461 ttatacaacc cttctgcggt cagccctttt ccgctggtaa acatccagtg cccggcatag
     5521 agactgtttt cgctgccctg cgggcttaaa gtgatggact gacttttgtt gtactgtcca
     5581 cccataatga tcacgctgtc atgttcttga tcgatcaggt aatttgccag cggtagataa
     5641 ttagtgatga tttgcactgg cttgccacac atttcccgcc caagcagaaa cgcggtggag
     5701 ccgcagttga tgactacgct ttcgcccgga ttaaccagct gcgaggccgc tttagcaata
     5761 cgtacttttt catcgtgatt ctgcgcctga tgcagattca tcggcgtcca gcgcgggcgc
     5821 tgttgggtaa tagcttctgc gccattgcgc acctttttca gtttgccgcg ttcgtcaagt
     5881 ttattgatat cgcgtcgcgc agtggcaggc gaaattccca gacgctcaac gactttctca
     5941 acggtcacaa agcccaattg tgcgagcatt tccaggagga tttgatgtct ttgtgcttca
     6001 gtcatgagct attccgataa aatttgattt ttttagatga tatttgaaat agctaggaaa
     6061 tactacgccg gatagcgcga acttctccac gctatccggt ataaacgaca gattacagga
     6121 atgacttgaa cggcagatcc ggttcaatag tgaagcaatc atcgaaaccg cgcggatagt
     6181 ggtactcgaa gttgtcttta tccagcggcc aggtaaattt gccgccaacc tgccagataa
     6241 acggcttgaa gccatacttc agacgatctt ttttcatctc ccacagcacg cggatctctt
     6301 gcggatcggc ctggaagttt gaccagatgt cgtggtggaa cgggataacc actttcgcat
     6361 tcagggcttc acccatacgc agcatatcgg cgctggtcat tttgtcggta ataccgcgcg
     6421 ggttctcacc atacgatcct aacgccacat cgatctgatg ttcgttaccg tgcttcgcat
     6481 aatagttaga gtagtgggaa tcgccactgt gatataggga accgccaggg gttttgaaca
     6541 ggtagtttac tgcgcgatcg tccatgccat ctggcagtac gccagccgct ttttgatcgg
     6601 caggcagggt gatcagtgca gtacggtcga aagcgtcaag cgcatgaatt tcaatgtctt
     6661 tcacttttac aacatcgccc ggtttgacca cgatgcaacg ctctttcggt acgccccagc
     6721 caatccacaa atcaacgcag gttttcggtc caatgaatgg tacgtcatca gcacagttct
     6781 gcatcacggc agcagcgacg ttaacgtcga tatgatcgtt gtgatcgtga gtcgccagta
     6841 ccgcgtcgat ctggcgaatc gcaaacggat caagaacaaa cggggtggtg cgtaggtttg
     6901 gctgcagttt tttcacgcca gccatgcgct gcatctggtg accctgtttc attaacgggt
     6961 taccgtgact ttgtttgcca gtgccgcacc agaaatcaac gcaaacgttg gtgccacctt
     7021 ctgatttcaa ccagatcccg gtgcagccta gccaccacat cgcaaatgtg ccaggggcga
     7081 cctgctcttg ttcaatttct tcattcagcc agctacccca ctccgggaaa gtgctcagga
     7141 tccaggattc tcgggtgata cttttcactt tactcatcgc catttacctt catgatagtt
     7201 caattcgaat caatatgtga ttggttttga ttaatcctga cactattttt tcaggaaggc
     7261 aatgaccatt ttttgacttt ttgccaggga agttgttgtt gattttgagt gtggaaagat
     7321 ttaatggaat gtgtagttaa attaactaaa tgaatttaaa taaataattg tttcgttgtg
     7381 tgaatcccac tctatccgtg tggaattatt tgcgggtcgc gtcacattta atcataaata
     7441 atcttgttgt gattactttt gaaaattaga gtgagtgcac aacattccgg gtgtgcggaa
     7501 tacccggtta cctcttcttc aggagatcgt tatggagatc ctctacaaca tctttaccgt
     7561 gttttttaac caggtcatga ccaatgcccc gttgttgctg ggtattgtga cctgtctggg
     7621 ctacatccta ctgcgcaaaa gtgtcagcgt tattattaaa ggcacgatta aaaccataat
     7681 tggtttcatg ttgttgcagg cagggtccgg catcctcacc agcaccttca aaccggtggt
     7741 ggcgaaaatg tccgaagtct acggcattaa acggcgcaat ttccgatact tacgcgtcga
     7801 tgatggcaac catcgaccgc atgggcgatg cctatagctg ggtaggttac gcggtgttgt
     7861 tagcgctggc gctgaacatc tgttacgtgc tgctgcgtcg cattaccggt attcgtacca
     7921 tcatgctgac tggtcatatc atgttccagc aggcggggct gattgccgtt acgttgttta
     7981 ttttcggcta ctccatgtgg acaaccatta tctgcacggc gattctggtt tcgctctact
     8041 ggggcattac ctccaacatg atgtacaagc cgactcagga agtgacggac ggctgcggtt
     8101 tctccatcgg tcaccagcaa cagtttgcat catggactgc ctataaagtt gcgccgttcc
     8161 tcggcaaaaa agaggaaagc gttgaagacc tcaaactgcc aggctggctg aatattttcc
     8221 acgacaacat cgtctccacg gcgattgtca tgaccatctt ctttggtgcc attctgctct
     8281 ccttcggtat cgacaccgtg caggcgatgg caggcaaagt gcactggacg gtctatatcc
     8341 tgcaaaccgg tttttccttc gcggtggcga tcttcatcat cactcagggt gtgcgcatgt
     8401 ttgtggcgga actctctgaa gcgtttaacg gtatctccca gcgcctgatc cctggtgcgg
     8461 ttctggcgat tgactgtgcg gctatttata gcttcgcgcc gaacgccgtg gtctggggct
     8521 ttatgtgggg caccatcggt cagctgattg cggttggcat cctggtcgcc tgcggttcct
     8581 cgatcctgat tatcccaggc tttatcccga tgttcttctc caacgccacc atcggcgtgt
     8641 tcgctaacca cttcggcggc tggcgtgcgg cgctgaagat ttgtctggtg atggggatga
     8701 tcgaaatctt tggttgcgtc tgggcggtga aacttaccgg tatgagtgcc tggatgggga
     8761 tggcggactg gtcgattctg gcaccgccga tgatgcaggg cttcttctcc atcggtatcg
     8821 cctttatggc cgtcatcatt gtaattgcac tggcttatat gttcttcgct ggccgcgcac
     8881 tgcgcgcaga agaagatgca gaaaaacaac tggcagaaca gtctgcttaa taaggagttt
     8941 tgattatgac cgtacgtatt ctggctgtgt gtggcaacgg acaaggcagt tccatgatca
     9001 tgaggatgaa agtggaccag tttttaaccc aatcaaacat tgaccacacg gtaaatagct
     9061 gcgcggttgg cgaatacaaa agcgagttga gcggcgcgga tatcatcatc gcttctacgc
     9121 acattgcggg cgaaatcacc gtgaccggca acaaatacgt ggttggcgtg cgcaacatgc
     9181 tctctcctgc cgactttggc ccgaaactgc tggaagtgat caaagcgcat ttcccgcagg
     9241 atgtgaagta aggacgcgcc atgaaattac gtgattcgct ggcggagaat aaatccatcc
     9301 gcctgcaggc tgaagctgag acatggcagg acgcagtaaa aatcggcgtt gacctgctgg
     9361 tggcggatgt tgtcgagccg cgttactacc aggcgattct ggatgcggtt gaacagcacg
     9421 gtccgtactt tgtgctcgca ccaggcctgg caatgccgca cggtcgcccg gaagagggcg
     9481 ttaagaaaac cggttttgcg ctggtgacgc tgaaaaagcc gctggagttc aaccacgaag
     9541 ataacgaccc ggtggatatc ctcatcacca tggcggcggt cgatgccaac acgcaccagg
     9601 aagtgggcat catgcagatc gtcaacctgt ttgaagatga agaaaatttc gaccgtttac
     9661 gtgcctgccg taccgagcag gaagtactgg atctcattga ccgcaccaac gcggcagctt
     9721 aagaaggaat tgaagatgtc attaccgatg ttgcaagtcg cgctggacaa ccagactatg
     9781 gatagcgcct acgaaaccac tcgcctgatt gccgaagaag tcgacattat cgaagtgggt
     9841 accattctgt gcgtgggcga aggcgtgcgt gcggttcgcg atctgaaagc gctctacccg
     9901 cacaaaatcg tgctggcaga cgccaaaatt gccgatgcgg gcaaaattct ttctcgtatg
     9961 tgcttcgaag ccaacgctga ctgggtgacc gtgatttgtt gtgcggatat caacaccgcc
    10021 aaaggtgcgc tggacgtagc aaaagagttt aacggcgacg tgcagatcga actgaccggt
    10081 tactggacct gggaacaggc gcaacagtgg cgcgacgcag gtattcagca ggtggtttat
    10141 caccgcagcc gtgacgcgca ggccgctggc gtggcgtggg gtgaagccga tatcactgcg
    10201 atcaaacgtc tttccgatat gggcttcaaa gtcaccgtca ccggtggcct ggcgctggaa
    10261 gatctgccgc tgttcaaagg cattccaatt catgtgttta tcgccggtcg cagcatccgt
    10321 gatgccgctt ctccggttga agccgcgcgt cagttcaaac gttccatcgc cgaactgtgg
    10381 ggctaaggag cggatatgtt gtccaaacaa atcccgcttg gcatctatga aaaagcgctc
    10441 cccgccgggg agtgctggct ggaacgcctg cgactggcaa aaacgttggg cttcgatttt
    10501 gtcgagatgt cggtagatga aactgacgag cgcctgtcgc gcctcgactg gagccgcgag
    10561 cagcgtctgg cgctggtgaa tgcgattgtt gaaaccggcg tgcgtgtgcc gtccatgtgc
    10621 ctttctgccc atcgtcgttt cccgttgggc agcgaagatg acgcggtgcg ggcgcagggg
    10681 ctggagatta tgcgtaaagc catccagttc gcccaggacg ttggcattcg cgtgatccag
    10741 ctggcgggct atgacgttta ctatcaggaa gccaataacg aaacgcgtcg tcgtttccgt
    10801 gacggcctga aagagagcgt tgagatggcg agccgcgcgc aggtgacgct ggcgatggag
    10861 atcatggatt atccgttgat gaactccatc agcaaggcgc tgggttacgc gcactatctc
    10921 aacaacccgt ggttccagct ctacccggat atcggcaacc tgtcggcgtg ggacaacgat
    10981 gtgcagatgg agttgcaggc cggaatcggg catatcgtcg cggtgcatgt gaaagacacc
    11041 aaaccgggcg tcttcaaaaa cgtgccattt ggcgaaggtg tggtggattt cgaacgttgc
    11101 ttcgaaacgc tcaaacagag tggctattgc gggccgtacc tgattgagat gtggagcgaa
    11161 acggcggaag acccggcggc agaagtggtg aaagcgcgtg attgggtgaa agcgcgcatg
    11221 gccaaagcag gcacggtgga ggcggcataa tgcaaaagct aaaacagcag gtgtttgaag
    11281 ccaacatgga tctgccgcgc tatgggctgg tgacctttac ctggggcaac gtcagcgcta
    11341 tcgaccgcga acgcgggctg gtggtgatca agcccagcgg cgttgcctac gaaaccatga
    11401 aagcagacga tatggtggtg gtcgatatga gcggcaaggt ggtggaaggg gagtatcgcc
    11461 cgtcttccga caccgcgacg catctcgaac tctaccgtcg ttacccgtcg cttggcggca
    11521 ttgtccatac ccactccact catgctactg catgggcgca ggcggggctg gcgatcccgg
    11581 cgttaggcac tacgcacgcc gactacttct ttggcgacat cccgtgtacg cgcggattaa
    11641 gcgaagaaga ggtgcagggc gagtatgaac tgaacaccgg caaagtgatt atcgaaacgc
    11701 tgggtgacgc cgagccgctg catacgccgg ggattgtggt gtatcagcac ggtccgttcg
    11761 cctgggggaa agatgcccac gacgcggtgc ataacgcggt ggtgatggaa gaagtggcga
    11821 aaatggcgtg gattgcgcgc agtattaacc cacaactgaa tcacatcgac agcttcctca
    11881 tgaataaaca cttcatgcgt aagcacggtc ctaacgccta ttacgggcag aagtaagcag
    11941 tgcgccccgg aattattacc tcctgcggga tgtaattggt ctgattccgg ggccgcttca
    12001 gttaaaaaat cgtataaaca acaggtgttt ttatggctat caatggattg tttgtcagaa
    12061 cggatccgca acgtcgccgc tatggcgttg cgttacttgt gggtgtactt tcaggaatta
    12121 tttcagcatt tgttaaatgg ggagcggaag tccccttgcc accgcgcaca ttttccggtg
    12181 gtcgcgatga atttaacccg ccgtatatct ttctacggga ttatttaggt atcgacccaa
    12241 cccaaactgt gtatacattc tcggaacata ttatcaaccc ggtgatggtg acgcatatca
    12301 tcttctcact ggtttttgcg atagggtatt gtattgtcgc ggaaattttc ccgaaagtga
    12361 aattatggca ggggatatta gcagggctta ttgtgacagt ggcagtccac ggtatatttt
    12421 gcccggcgct gaatttaacg ccgccgctca ctcaattgcc ttttgacgaa tatgcctctg
    12481 aaatcctggg gcatattttc tggttctgga taatagaaat tatgcgccgc gacttacgta
    12541 atcgcattac tcatgagcca gatccggaag tcgcaatccg gtgaataacc gcataaaaaa
    12601 gccccctgcg aagggggcaa agcaaactat ggcaatgttt cgttggttat acctggtgct
    12661 agcgataaat atccgcgctg gcgtgcatat tgccgttact ccccggttcc cgcatcagaa
    12721 ttacgtggta gtacgttgcg ccctgcgcat ctgtctcttc atttaatgcc tgacgcgctt
    12781 cgctttcagt ggcgaaatta tgattgatat aaatcacgcc taagctttgc acatcatcca
    12841 tgtttctggc ctggtggtta ttaatttcaa tggctgccca tgtatttgca cttagcaaaa
    12901 gcacagccag aagggctaaa actcgactga acatagatac ctcctcgacg gctgactttg
    12961 tgtgctctcc tctgtgatga tcttctgatt taattttaat caatgataaa gaagttgatg
    13021 gtgaccattt ctgatgcagt tgttcaaaaa aacaccatga tgaagtgtga tgaactccaa
    13081 atcagcggtt tagaggttaa ttgcgaaagg ggagatttat ttcggctctg cccttgagtt
    13141 tagcggggca tacaagtact ataacgcgtc atttttcagc cgacctttaa cacgttcctt
    13201 gcctccccgg gattcggctg acccagacag gaggctgaat aatccgtaag gagcaattcg
    13261 atgcgtcatt acgaaatcgt ttttatggtc catcctgacc agagcgaaca ggttccgggc
    13321 atgatcgagc gctacactgc tgccatcact ggtgcagaag gcaagatcca ccgtctggaa
    13381 gactggggcc gccgtcagct ggcttacccg atcaacaaac tgcacaaagc acactacgtt
    13441 ctgatgaacg ttgaagctcc gcaggaagtg atcgatgagc tggaaactac cttccgcttc
    13501 aacgatgccg ttatccgcag catggttatg cgtaccaagc acgctgttac cgaagcatct
    13561 ccgatggtta aagcgaaaga cgagcgccgt gagcgtcgcg atgatttcgc aaacgaaacc
    13621 gctgatgatg ctgaagctgg ggattctgaa gagtaatttc tgatgaccaa ccgtctggtg
    13681 ttgtccggca ccgtgtgcag gactcccctt cgaaaggtca gtccatcagg aattcctcac
    13741 tgccagttcg tgcttgagca tcgttctgtg caggaggaag ccggttttca ccggcaggcg
    13801 tggtgccaaa tgcccgttat tgttagcgga cacgaaaacc aggccattac tcacagtata
    13861 acggtcggca gtcgcataac cgttcagggg ttcatttcat gccacaaggc aaagaacgga
    13921 ctgagcaaaa tggttttgca tgccgagcag attgaattga tagattctgg agactagcca
    13981 tatggcacgt tatttccgtc gtcgcaagtt ctgccgtttc accgcggaag gcgttcaaga
    14041 gatcgactat aaagatatcg ctacgctgaa aaactacatc accgaaagcg gtaagattgt
    14101 cccaagccgt atcaccggta cccgtgcaaa ataccagcgt cagctggctc gcgctatcaa
    14161 acgcgctcgc tacctgtccc tgctgccgta cactgatcgc catcagtaat cggtcacggt
    14221 ccattaatac gactttgaga ggataaggta atgcaagtta ttctgcttga taaagtagca
    14281 aacctgggta gcctgggtga tcaggtaaac gttaaagcgg gctatgctcg taacttcctg
    14341 gtaccgcagg gtaaagctgt tccagctacc aagaaaaaca ttgaattctt cgaagcacgt
    14401 cgcgctgaac tggaagctaa actggctgaa gttctggcag ctgctaatgc tcgcgctgag
    14461 aaaatcaatg cactggaaac tgttaccatc gcgtctaaag ctggcgacga aggtaaactg
    14521 ttcggttcca tcggtactcg cgacatcgct gacgctgtaa ctgcagctgg cgttgaagtg
    14581 gctaagagcg aagttcgtct gccgaacggc gttctgcgta ccactggcga gcacgaagtg
    14641 agcttccagg ttcacagcga agtattcgcg aaagtgatcg taaacgtagt agctgaataa
    14701 ttcgttattc aacgagacgt aaaaacgccg accagtggtc ggcgttttgc ttttgtagcg
    14761 agcacttgca gatatcgata agggccacaa taagcagccc tacgaagata attacttagc
    14821 tgcctgaata tcagccagca ttggggcaag ggctgggttg tccttagcga agtttgcgta
    14881 cattggctgt actttctcga taaacggagc tttatctacc tggtagactt tcacgcccat
    14941 tttatccagt tttgcaaatt cttgtttctc ttcctcgctc cacaagtctt tcatgtagct
    15001 cattgattct ttagctgctt ttaatataac tgcctgattt tccgggctaa gtttatcaaa
    15061 cgcagcattg ctcatcagaa ccacatcggg aaccatagtg tgttcgtcaa ggctgaagga
    15121 ttttgccact tcaccgtgac gcatggtggt cagcgccatc acgctgtttt ctgccatatc
    15181 gaccacaccc tgctgtaagg ctgtatacaa ctcgccgtaa tccagtggcg tcgggttacc
    15241 gcccatgact ttgatcattt caactgcgcc agggcttggc tggacacgga ctttcatgcc
    15301 tttcagatcg tctggtttca gtactggttt attgccatag aaactgcggg cgcctccatc
    15361 ataaaaagtc agcccgaaat aacctttgct tttcgttgag tcgaggattt ttctcccgat
    15421 atcgccctgc atgacctgat aataatgatc acgatcgcgg aacaaataag gcaggctaaa
    15481 taatttatag gaattttcaa atgcttccat ttctgccgca ttggttttta ccagtggaat
    15541 agcgccagaa cgaatcagct ccattgtttc tcgttgagta cccaatgttc catttggata
    15601 aatacgaata gtaatatcac cgttagagta ctctttgttc tttttcgcaa agaactccat
    15661 cgctttatga acaggatgag acttatcctg attatgactc agttttaatg tcactgccca
    15721 ggactggctt gcggataaca taagaacagc accgataatg ccagcggata cttttatttt
    15781 catagatgtt ccttttctaa taaatatggc ggcaggtgcc gccagtgaat gcgcatcagt
    15841 agcccagtgc ttttggcaat gccatactga tttgcggaat ataggtcacc attgccagca
    15901 ccaataccat cacggcatag aatggcagca taggtttgat aattttatta atcggtaatt
    15961 tagatacact acaaccgaca aataacacac tgcctacagg cggagtacaa ataccgatac
    16021 acatgttcag taccatgaca ataccaaagt gaatcgggtc catgcccagt tcggtgacaa
    16081 caggtaaaaa tatcggcgta aaaatcagta ccgccggtgt catatccata aaagtgccga
    16141 taatcaacag gataatattg ataataagca ggattattat tgggttgtca gaaatagtgt
    16201 taagcaaatc gagaatcagg aagggaacgt cagcgtttga cattgcccat ga
//
LOCUS       DAZPLI010000086        16048 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZPLI010000086 DAZPLI010000000
VERSION     DAZPLI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.087"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(36..1859)
                     /gene="typA"
                     /locus_tag="ACHUU3_002657"
     CDS             complement(36..1859)
                     /gene="typA"
                     /locus_tag="ACHUU3_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR2082681.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            2232..3641
                     /gene="glnA"
                     /locus_tag="ACHUU3_002658"
     CDS             2232..3641
                     /gene="glnA"
                     /locus_tag="ACHUU3_002658"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR2082682.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            3815..4864
                     /gene="glnL"
                     /locus_tag="ACHUU3_002659"
     CDS             3815..4864
                     /gene="glnL"
                     /locus_tag="ACHUU3_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR2082683.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            4876..6285
                     /gene="glnG"
                     /locus_tag="ACHUU3_002660"
     CDS             4876..6285
                     /gene="glnG"
                     /locus_tag="ACHUU3_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR2082684.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            6397..6507
                     /locus_tag="ACHUU3_002661"
     CDS             6397..6507
                     /locus_tag="ACHUU3_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR2082685.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(6697..8070)
                     /gene="hemN"
                     /locus_tag="ACHUU3_002662"
     CDS             complement(6697..8070)
                     /gene="hemN"
                     /locus_tag="ACHUU3_002662"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR2082686.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8259..8768)
                     /gene="yihI"
                     /locus_tag="ACHUU3_002663"
     CDS             complement(8259..8768)
                     /gene="yihI"
                     /locus_tag="ACHUU3_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR2082687.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(8887..9053)
                     /locus_tag="ACHUU3_002664"
                     /pseudo
     CDS             complement(8887..9053)
                     /locus_tag="ACHUU3_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9350..9982
                     /gene="yihA"
                     /locus_tag="ACHUU3_002665"
     CDS             9350..9982
                     /gene="yihA"
                     /locus_tag="ACHUU3_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR2082688.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(10364..13150)
                     /gene="polA"
                     /locus_tag="ACHUU3_002666"
     CDS             complement(10364..13150)
                     /gene="polA"
                     /locus_tag="ACHUU3_002666"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR2082689.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            13514..14446
                     /locus_tag="ACHUU3_002667"
     CDS             13514..14446
                     /locus_tag="ACHUU3_002667"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR2082690.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(14487..15917)
                     /locus_tag="ACHUU3_002668"
     CDS             complement(14487..15917)
                     /locus_tag="ACHUU3_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR2082691.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         3804 a         4097 c         3941 g         4206 t
ORIGIN      
        1 agcgatggca ggttttttat aactaaaaaa cgaaattaat cgtctttcgg tgcgcggttg
       61 gcgcgacgac gatcgttttc cgtcaggtga cgtttacgaa tacggataga ggtcggagtc
      121 acttctacca gttcgtcatc atcgatgaac tccagagctt gttccagagt catgcggata
      181 ggcggaacca gaacaacagc ttcgtcagta ccggaagcac gcatgttggt cagtttctta
      241 ccggtcaggc agtttacagt caggtcgtta gagcggctgt gaataccgat aatctgacct
      301 tcgtaaactt ctgcaccgtg accgaggaac agcttaccgc ggtcctgcag accgaacagt
      361 gcgaacgcga ccgctttacc ctgaccgtta gagatcagta cgccgttctg acgctgaccc
      421 acttcacccg gacgtacgtc gtcgtagtgg ctgaaggtgg agtacagcag accagtaccg
      481 gaagtcatgg tcatgaactc agaacggaag ccaatcagac cacggcttgg gatcacgtag
      541 tcgagacgta cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc tttacgttcg
      601 cccagcgcct gcattacaga accctgatgc tgttcttcaa cgtccagagt cacgttttca
      661 tacggctctt gtttacgacc gtcgatttca cggaagatta cttttggacg ggataccgcc
      721 agttcgaaac cttcacgacg catgttttcg atcagaacag acaggtgcag ttcgccacga
      781 ccagaaacgc ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc aacgttgtgt
      841 accagttctt tgttcagacg atccaggatc tgacgagacg ttacgaactt accttcttta
      901 ccgcagaacg gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc atcaacggag
      961 agtgccggca gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat gttcagttcg
     1021 ccaaggcccg tgatcgccac gatatcgcca gcttccgcca gatcggtttc gatacgttcc
     1081 agaccgaggt ggcccagcac tttaccgact ttcgcgttgc gggttttgcc ttcgctatcg
     1141 atgatagtga cctgctggtt cggcttcact ttaccgcgct tgatgcggcc aatgccgata
     1201 acgccaacat agctgttgta atcgagctga gaaatctgca tctggaacgg accgtcaagg
     1261 tcaacgtccg gcgcaggaac gtggtcaaca atcgcctggt acagcggggt catgtcttcc
     1321 gccatatctt cgtggtccag acccgcgata ccgttcagcg cagaagcgta aacgatcggg
     1381 aagtccagct gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac ctgatccaca
     1441 acccaatcag gacgcgcgcc agggcggtca actttgttga taacaacaat cggcttcagg
     1501 ccgtaagcaa acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc gtcaaatgcg
     1561 tcaaccacca gcagcactga gtctaccatg gacattacac gttcaacttc accaccgaag
     1621 tcggcgtgcc ccggggtatc aacgatgttg atacggtaat cattccattt gatagcggtg
     1681 tttttcgcga ggatggtaat cccacgctct ttctccaaat cgttggagtc catcacgcgc
     1741 tcttgggttt cggcacgaga gtcgaacgta ccggattgtt ggagcagctt gtctaccagg
     1801 gtggttttac catggtctac gtgcgcgatg atggcgatat tacgcaattt ttcgatcaca
     1861 actttgcctc aggcattaga aatagcgcgt tattgtacac ggattaatcg cactacaaaa
     1921 caggatcaca aacatcctcc gcaaacaagt attgcagagt ccctttgtga tcgctttcac
     1981 ggagcataaa aagggttatc caaaggtcat tgcaccaaca tggtgcttaa tgtttccatt
     2041 gaagcactat attggtgcaa cattcacatc gtggtgcagc ccttttgcac gatggtgcgc
     2101 atgataacgc cttttagggg caatttaaaa gttggcacag atttcgcttt atctttttta
     2161 cggcgacacg gccaaaataa ttgcagattt cgttaccacg acgaccatga ccaatccagg
     2221 agagttaaag tatgtccgct gaacacgtac tgacgatgct gaacgagcac gaagtgaagt
     2281 ttgttgattt gcgcttcacc gatactaaag gtaaagaaca gcacgtcact atccctgctc
     2341 atcaggtgaa tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc tcaattggcg
     2401 gctggaaagg cattaacgag tccgacatgg tgctgatgcc agacgcatcc accgcagtga
     2461 ttgacccgtt cttcgccgac tccaccctga ttatccgttg cgacatcctt gaacctggca
     2521 ccctgcaagg ctatgaccgt gacccgcgct ccattgcgaa gcgcgccgaa gattacctgc
     2581 gttccactgg cattgccgac accgtactgt tcgggccaga acctgaattc ttcctgttcg
     2641 atgacatccg tttcggatca tctatctccg gttcccacgt tgctatcgac gatatcgaag
     2701 gcgcatggaa ctcctccacc caatacgaag gtggtaacaa aggtcaccgt ccggcagtga
     2761 aaggcggtta cttcccggtt ccaccggtag actcggctca ggatattcgt tctgaaatgt
     2821 gtctggtgat ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa gtagcgactg
     2881 ctggtcagaa cgaagtggct acccgcttca ataccatgac caaaaaagct gacgaaattc
     2941 agatctacaa atatgttgtg cacaacgtag cgcaccgctt cggtaaaacc gcgaccttta
     3001 tgccaaaacc gatgttcggt gataacggct ccggtatgca ctgccacatg tctctgtcta
     3061 aaaacggcgt taacctgttc gcaggcgaca aatacgcagg tctgtctgag caggcgctgt
     3121 actacattgg cggcgtaatc aaacacgcta aagcgattaa cgccctggca aacccgacca
     3181 ccaactctta taagcgtctg gtcccgggct atgaagcacc ggtaatgctg gcttactctg
     3241 cgcgtaaccg ttctgcgtct atccgtattc cggtggtttc ttctccgaaa gcacgtcgta
     3301 tcgaagtacg tttcccggat ccggcagcta acccgtacct gtgctttgct gccctgctga
     3361 tggccggtct tgatggtatc aagaacaaga tccatccggg cgaagccatg gacaaaaacc
     3421 tgtatgacct gccgccagaa gaagcgaaag agatcccaca ggttgcaggc tctctggaag
     3481 aagcactgaa cgaactggat ctggaccgcg agttcctgaa agccggtggc gtgttcactg
     3541 acgaagcaat tgatgcgtac atcgctctac gtcgcgaaga agatgaccgc gtgcgtatga
     3601 ctccgcatcc ggtagagttt gagctgtact acagcgtcta atagttgaag ttgtactacc
     3661 cggcgcaaca acgccgggat ttagttgccg tggaaacttt cagcccatct ctgcatgggc
     3721 ttttttctcc gtcaattctc tgatgcttcg cgctttttat ccgtaaaaag ctataatgca
     3781 ctaaaatggt gcaacctgtt caggagactg ctttatggca acaggcacgc agcccgatgc
     3841 tgggcagatc ctcaactcgc tgattaacag tattttgtta attgatgaca atctggcgat
     3901 ccattacgcc aaccccgccg cgcaacaact gctcgcccaa agctcccgca aattgtttgg
     3961 tacaccgtta ccggaactgt tgagctactt ctcattaaat atcgagctga tgcaagaaag
     4021 tctggaggcg gggcaaggtt ttaccgataa cgaagtgacg ctggtcatcg acgggcgctc
     4081 gcatatcctt tctgtgacgg cccagcgtat gccggacggc atgatcctgc tggagatggc
     4141 tccgatggat aaccagcgcc gcttaagtca ggaacagcta cagcacgccc agcaggttgc
     4201 tgcccgtgat ttagtgcgcg gcctggcaca tgagattaaa aatccgcttg gaggtttacg
     4261 tggcgcggcg cagctgctca gcaaagcgtt acctgaccca tcactactcg aatataccaa
     4321 agtgattatc gaacaggcgg accggctgcg aaatctggtc gaccgtctgt tggggccgca
     4381 gctgcccggt acgcgcgtta ccgaaagtat tcacaaagta gctgaacgcg tggtgacgct
     4441 ggtgtcgatg gaactgccgg acaacgtgcg gttgattcgt gattacgacc ccagcctgcc
     4501 ggaactggcg cacgacccgg atcaaattga acaggtcttg ctgaatattg tgcgcaatgc
     4561 gctacaggcg ctggggccgg aaggcggtga aatcattctg cgtacccgca ccgcgtttca
     4621 actgacctta cacggcgagc gctaccggct ggcggcgcgg attgatgtgg aagataacgg
     4681 gccgggcatt ccgcctcatt tgcaggatac gctgttttac ccgatggtca gcggccgcga
     4741 aggtggcacc gggcttggct tatccatcgc ccgtaatttg attgatcagc attcaggcaa
     4801 aattgaattt accagttggc caggtcatac cgagttctcg gtttacctgc ctatcaggaa
     4861 ataaaggtga cgtttatgca acgagggata gtctgggtag tcgatgacga tagttccatc
     4921 cgttgggtgc ttgaacgtgc gctcgctgga gcgggtttaa cctgtacgac atttgagaac
     4981 ggcgcggaag tactggaggc gctggcgagc aaaacgccgg atgtgctgct ttcagatatc
     5041 cgtatgccgg gaatggacgg gctggcgctg ctcaagcaga ttaaacagcg ccatccaatg
     5101 cttccggtca tcattatgac cgcacattcc gatctggatg ctgccgtcag cgcctatcaa
     5161 caaggggcgt ttgattatct gcccaaaccg tttgatatcg acgaagcagt ggcgctggtt
     5221 gagcgcgcta tcagtcatta ccaggaacag cagcagccgc gtaatattca gcttaacggc
     5281 ccaacgaccg atatcatcgg cgaagcgcca gccatgcagg acgtgttccg tattatcggt
     5341 cggctttcgc gttcttctat tagcgtgctg attaacggcg aatccggcac cggtaaagaa
     5401 ctggtcgctc atgccctgca tcgccacagt ccgcgcgcca aagcgccgtt tatcgcgctg
     5461 aatatggcag ctatcccaaa agatttgatc gaatcagaac tgtttggcca cgagaaaggc
     5521 gcgtttactg gcgcgaatac cattcgtcag gggcgttttg aacaggccga tggcggtaca
     5581 ttattcctcg acgaaattgg tgatatgccg ctggatgtgc agacgcgttt gctgcgcgtg
     5641 ctggcagacg gtcagtttta ccgcgttggc ggctatgcgc cggtgaaagt ggatgtgcgt
     5701 attatcgctg ccactcacca gaatctcgaa caacgagtgc aggaaggtaa gttccgtgag
     5761 gatctgttcc accgcctgaa cgttatccgc gttcatctgc cgccgctgcg cgaacgtcgg
     5821 gaagatattc cccgtctggc gcgccatttt ttacaggttg ccgcgcgcga actgggcgta
     5881 gaagcgaagt tactgcatcc ggaaaccgaa gctgctctga cgcgtctggc gtggccaggc
     5941 aacgtgcgcc agctggaaaa cacctgccgc tggctaacgg tgatggccgc cgggcaggaa
     6001 gtgttgattc aggatttgcc cggcgaactg tttgaatcaa cggttgcgga gagtacttcg
     6061 caaatgcaac cggacagctg ggcaacgctt cttgcgcagt gggcagacag agcgctgcgt
     6121 tccggtcatc aaaatctgct ttccgaagcg cagccagagc tggagcggac gttactgacg
     6181 accgcgttgc gacatacgca ggggcataaa caggaagcgg cgcggctact cggctggggc
     6241 cgcaacaccc tgacgcgtaa gttaaaagag ctggggatgg agtgattcac agcttgtgtg
     6301 taaagattga ttattgagcg caaattgctg gtattttacg ctttactgtt ccgataagtt
     6361 cagtatgatc ttgcccggaa aacggggaga gtcattatgc tggaatcaat aattaatctg
     6421 gtatcgagtg gcgcagttga cagccacaca ccgcaaactg ccgttgctgc cgtgctgtgt
     6481 gccgcaatga ttgggctgtt tagttgagtt tcagattgct gatgaagtgc tctttgttca
     6541 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag caatattttc aacaggtttt
     6601 gtaggcctga taagcgtagc gcatcaggca atcttgttct cagcacattg atggcgcttt
     6661 cgttttctac tttgtaaacg aagcgccatt cactatttaa atcacccgtg agaactgctg
     6721 catccgcgct ttctggcgca gataggtatc aaagcacatg caaatgttgc ggatcagcaa
     6781 gcgacctttc gccgtcacct gtattccctt ctcatccaca tccaccagcc catcttttgc
     6841 taacggggcg agcagcttga gatcttccgc aaagtaatca gcgaagtgca aatcccactg
     6901 tttctcaata ggggcgtaat ccagacggaa gttgcagatg agcgacttaa tcacatcgcg
     6961 gcgaatacag tcatcacgcg ttagcgcaat accacgccac agcgcattgc cttgttcatc
     7021 cacttgctga tagtactgct tcaactcttt ctggttctgc gcgtagcagt cgccaatcat
     7081 gctgatggcg gaaacgccca tccccagcag atcggtatcg ccctgagtgg tgtagccctg
     7141 gaagttacga tgcagcacgc cttcacgctg ggccaccgcc agctcgtcat ccggacgggc
     7201 aaagtgatcc ataccgataa actgatagcc cgattgcgtc aggaaggcga tggtttcctg
     7261 caggatatcg agtttttgct gcggactcgg caggtcagca tctttgattt tgcgctgagc
     7321 agcaaaaatg gtcggcagat gcgcgtagtt aaagacactc agacgatcgg ggttcagctc
     7381 cgccacacgt ttcagggtaa aggcgaaact ctccggcgtc tgtttcggca ggccgtaaat
     7441 caggtcgatg ttggtggagg taaaaccaat ctcacgcgca tggttaagca gtgcaaagat
     7501 gaactcttca tcctgctcgc ggttaaccag acgttgcact tctttgttga agtcctgcac
     7561 gcccatgctc aggcgattaa agccttcggc gcgtaaatga tcgagtacat ccagttcgat
     7621 ttcccgcgga tcgacttcga tcgaaatctc cgcatcggca ttgaactgga agttttcgcg
     7681 cagcagcttc atcaggcggc tgatttgcgc tttattcaga tacgtcggcg ttccgccgcc
     7741 ccagtgcaat tggctgacgt gacgcccggc gaacagcggt gcacgatgga cgatttcttg
     7801 ctccagcgcg tccagatact gatcggcctt gtgctgctgg cgagtaacaa tcttattgca
     7861 accgcagaag taacaaagct tatggcagaa cgggatatgt acgtagagag ataatggacg
     7921 ctcaggatag cgcgccacgg cttgtaaaaa cgcctgttcg ccgaagtctt ctgaaaactc
     7981 cagcgcggtc gggtacgagg tgtatcgtgg cccggaatag ttatatttct ggatcagggc
     8041 cagatcccag tcgatttgct gtacagacat gctcactcct tccgatggcg tctctggcgg
     8101 ctacggcgta ccggcggatg cccgtttcgg gtcatcagcc ggttgcgcag ccacttttgt
     8161 cgccgcgaca accgctgtag tttaacgaat aaccacacca gataacatat aaccggaagg
     8221 gttatcagca gaacgggaag gcccacggtg gcaatccgtt agttaccccg cagcagacgc
     8281 atcatgtctt cttgcttctc gtcttcttcc tcttcttcgt catcgtcata agagaggccg
     8341 agtttctgca tcaactcatc aatacgatcc agtttagcat ccacccagga ttgctcttcg
     8401 gcgctcaggg tttcgcctgc ttccagacgt tccagcagcg catccagacg ctcatccgtt
     8461 tccagtaatt ccaactccgc ctgcggtgaa agcataggtt tctcactctt cggtttgtgc
     8521 tgtttggtga ctttttcagc cacgcccaat ggaatagggg ttttactgcc aatacgtgga
     8581 tcttttggtg cgttctggcc tttgctgcct gacgtggtgt ttccgcccgc tgcgcggctg
     8641 cccggcgcat gaccacgacg ttttttctga cgcttgcgat cacgagcttc ctgatccagc
     8701 tcctcgcgtg tttttcgacg cgcttttgca tgacctttgc tgcgtgagtt tgaagatgat
     8761 ggtttcataa tggttcgtct tttagccgtt ttattcagta tagatttgcg gcggaatcta
     8821 acagaaagca agcaaagaaa aaaggcgaca gattactctg tcgccttttt tcctgactca
     8881 taacccttaa cgggtcttac aatccttgca ggccgccaac acaccctgtt tttcctttag
     8941 ccggaacaat ccgtgttgat tccatttccg tttaattacg tctccggacg tttgtcttcc
     9001 tgacaatcct ctgtcttcgc ctcctggcgc tcctgaccct catcctgagt cattgttcct
     9061 gttagcgtcc tcgctctatg ggtgctactt tacgcctttg ctttaaacaa acaatcaacc
     9121 gccatcagta tttgcacatt ttcagagttt tatataaaag caattcactg atttatatag
     9181 cttatttgac atgcatcgca gaattttctc tgctatttcg cataaagact atcggcaatg
     9241 tctcacaaag cgtatggctt ttacttacaa cgctcacgcc gaaaaatccg ccttttatta
     9301 tggttcaggt ataatcgcca caaagctttg actgacggag acgaccgctt tgactaattt
     9361 gaattatcaa cagacgcatt ttgtgatgag tgcgcctgat attcgccacc taccttccga
     9421 taccggaatt gaagtggctt ttgcaggccg ttccaacgca ggtaaatcca gcgcgctgaa
     9481 cacgctgact aaccagaaaa gcctggctcg tacctcaaaa accccagggc gcacccagct
     9541 tatcaacctg tttgaagtgg ctgacggcaa gcgtctggtt gacttgcctg ggtacggtta
     9601 tgcggaagtc ccggaagaga tgaagcgcaa atggcagcgt gcgctcggcg aatacctcga
     9661 aaaacgtcag agcctgcaag gtctggtggt gctaatggat attcgccatc cgctgaaaga
     9721 tttggatcag cagatgattg agtgggcggt agacagcaat atcgccgttc tggtgctgct
     9781 gaccaaagcg gacaaactgg caagcggcgc acgtaaagcg caattgaata tggtgcgtga
     9841 agctgtactg gcgtttaacg gtgatgtgca ggttgaaacg ttttcttcgt tgaagaaaca
     9901 aggcgtggac aagctgcggc agaaactgga tacctggttt agcgagatgc agcctgtaga
     9961 agaaacgcag gacggcgaat aattttcttg ccttaatgct tgtgccggat gtggcgtatc
    10021 cggcccgtaa attcaataga ttacaattct accgttggcc tgataaacat agcgcatcag
    10081 gcattacgga tcttttcttt cgcccaataa aaaacgcccc agtcattact gactggggcg
    10141 gctaaaatat tcagccaaat ccgattacgt gaagtaaaag gtctgaaaga tagaacatct
    10201 tacctctgta ccctacgcga ctaactttac tcttttttgt tcagttcaga aagcactttt
    10261 tgtaattttt tttcagttgt tgcataggaa tttctaatag ccatcacaaa acgtgacagc
    10321 ttatgttgct tacttacgaa aaaagggcat gttcaggcga atcttagtgc gcctgatccc
    10381 agttttcgcc actccccact tccaccagca acggcacatc cagacgggta cagttttcca
    10441 tcagttgatg aatctgcttc gcgacggcat caacatcatc tttatgaact tcaaatacca
    10501 gttcatcgtg tacctgcatg atcatacgta cacgcggttg ctcagcctgt aaccacgcat
    10561 caacggcaat catcgcccgt ttgataatgt cggcggcggt tccctgcatt ggcgcgttaa
    10621 tggctgcacg ttcagccgct gcacgacgag caccattgct ggatttgata tccggcagat
    10681 acagacggcg tccgtccagc gtttcaacgt agccctgctc tttcgcctga gcacgggtgc
    10741 gttccatata ctccagcacg ccagggtagc gttcgaagta aaggtccatg tacttctgcg
    10801 cttctttacg tggaatgttc aattgccgcg ccagaccgaa agcactcatg ccataaatca
    10861 gaccaaagtt gatcgctttc gcgctacggc gttgctcgct ggtgacggtt tccagtggca
    10921 aaccaaacac ttctgccgcc gttgcccggt ggatatcttt tccttccgcg aatgcggtca
    10981 gcaagccttt gtcacgcgaa agatgcgcca taatgcgcag ttcaatctgc gagtagtccg
    11041 ctgagacaat cacataatcc tctggcgcaa taaacgcctg gcggatacga cgaccttctt
    11101 cgttacgcac cggaatgttt tgcaggttag gatcggttga cgataaacgt cccgttgcag
    11161 ttactgcctg gtgataagag gtatgcacac gcccggtttt cgggttgatc atcagcggca
    11221 gcttgtcggt gtaggtcgat ttcagcttcg ccagaccacg atactccaga atcacttttg
    11281 gcaacggata gtccagcgcc agttcttcca gtacctcttc cgacgttgac ggcgcgccac
    11341 ccggcgtttt cttcagcggt ttaatgccct gtttttcaaa cagaatggtt tgtaactgct
    11401 tggtggaaga aaggttaaat tcctcacctg caatttcatg cgctttcttt tccagctcag
    11461 ccagacgaag ggtgagctct tcagaatgat tgtgcagcac tttcggatcg atcttcacac
    11521 cgttacgttc aatgcgtgaa agcaccggca ccagcggcat ttcgatattc tcgaagacgt
    11581 tcaacggccc tttgtgtttt tgcagatctg gccacatttt cagatgcaac tgcaaggtga
    11641 catccgcatc ttcggcggcg taacgtccgg cctcttcgag ggcaatctgg ttaaaggtca
    11701 gttgattttt gcctttaccc gcaatctctt caaaagtgat agttttgtgc ttcaaccaac
    11761 gttccgcgag gctgtccata tcgtgacgcc cggcaacgct attgagaatg taggactcca
    11821 gcatggtatc aaacgcaatc ccacgcagct caatgccgta attcgccaga ataccgcgat
    11881 cgtatttcag gttttgcccg accttcagcg ccttttcatc ttccagcagc ggttttagca
    11941 actcgagtgc acgctcgcga gagatttgat cgggcgcatc aagataatca tgagcaaccg
    12001 gaatatatgc cgctacgcct ggctcgatag caaaagaaag cccgaccagg ttagcagaga
    12061 tgttatcaag gctgtcggtt tcggtatcaa atgcaaatac cggcgctttt tccagcttcg
    12121 caatccacgc tttcagtgtt tcttcatcaa ggatggtgac gtagttgtca taagaaatca
    12181 ccgttgccgt cacttctggc gcttcgtctg caacactggt ttcctgcggc ttcgcggctg
    12241 gttttgcccc tttggcctgt aaccatttgc ccgcttcgac atcagcagtc cagcgtttga
    12301 actcatactt tttgaacagc cccaacaact cttccgctgc cggttgctgc acttccagtt
    12361 gttcacaggt cagctccagt tcaacgtcgg ttttaatcgt cgccagctgg tatgagagat
    12421 aagcaacttc tttgttttgc tcgagcttcg ctgccattgt tttcgcgcca cggaagctca
    12481 acccagcaat tttttctggc tcggcataca gcgtatccag cccgccaaga ccttgcagca
    12541 atgcctgcgc ggttttttca ccgacgcccg gtacgccagg aatgttatcg gaggagtcac
    12601 ccatcagcgc caggaaatcg atgatcagtt ctggcggcac gccgtactta ttcaccacct
    12661 cttccggtcc gaggatggta ttcgtcatgg tgttgataag cgtaatattt ggcgtcacca
    12721 gctgcgccat atctttatcg ccagtgctga tcagcaccgg acgcccggct ttttcggctt
    12781 cgcgcgccag agtaccgata acgtcgtccg cttctacgcc agaaaccgcc agcagcggca
    12841 gtcccatcgc tttaaccatc gcgtgcaggg gttcgatttg tgctcgcaga tcgtccggca
    12901 ttggcgggcg atgtgatttg taatgttcaa acagttcatc acgaaaggtt tttcccttgg
    12961 cgtcaaagac caccgctgca tgcgtcggtt tatattgcat gatcagactg cgcagcatgt
    13021 tgaggacacc atacatcgca ccggtcggct cgcctgcgct gttagtcagc gggggaaacg
    13081 cgtgatatgc gcgataaaga taagatgaac catctacaag gataagtgga ttttggggga
    13141 tctgaaccat aatgtccgtg cctgtttatc agattatggg taaaggatgc cacagacagg
    13201 atgaaaacat gagtttttca ccgcttttcg tgaaaatctt ttgtagatct tctggatcgc
    13261 tcgcaaatcg tcatgtggat aactttgtgc ataaattatt tacatcgctt ttaatttatt
    13321 atcatgcttt ttacagcatg atgtttgtct ttgtttttca tgcagttaaa aataaagcca
    13381 tccctaataa ttgagataat gggatttttt ctaatgtgga tagatatgga ttatttttct
    13441 ccttaaggat catccgttat ttgggtcgtt ttctgctaaa atctgcgcct tgcgcttctt
    13501 tagcgcactg tctatggcta accttttgaa taaattcatt atgacgagaa tactcgctgc
    13561 gataaccctt ttgctgagta tcgtattgac cattttggtc actatttttt gttccgtccc
    13621 gatcatcatt gccgggattg taaaactatt gctacctgtg ccagtcattt ggcgaaaggt
    13681 ttcgcgtttt tgtgatttta tgatgtattg ctggtgtgaa ggtctggcgg tattactgca
    13741 tctgaaccca cacctgcaat gggaagttca cgggctggaa gggttaagta agaagaactg
    13801 gtatctgctt atttgtaacc accgtagttg ggcagatatt gtcgtactgt gcgtgctgtt
    13861 tcgtaagcac attccgatga ataaatattt cctcaagcaa cagctggcct gggtgccatt
    13921 tcttggcctg gcgtgctggg cgctggatat gccgtttatg aagcgttatt cccgcgctta
    13981 tttattacgt catccggagc gacgtggtaa agatgttgaa accactcggc gttcttgtga
    14041 aaagtttcgc ctgcatccca ccaccattgt caatttcgtt gaaggctccc gtttcacgca
    14101 agaaaaacat cagcaaaccc actccacttt tcaaaacttg ttgccaccaa aggcggcagg
    14161 cattgcgatg gcgctaaatg tactgggtaa acaattcgat aaactgctta atgttacgct
    14221 gtgttatccg gacaataacc gtcagccgtt cttcgatatg ttaagcggta aactgacgcg
    14281 gattgtcgta catgtggatt tacagcctat tgccgatgag ttacatggcg actacatcaa
    14341 tgacaaaagc tttaagcgcc attttcagca atggctgaac tcattgtggc aggaaaaaga
    14401 ccggcttctg acgtctctca tgtcatcaca gcgtcagaat aagtaagttt tactcgcctc
    14461 tgaatgagca gaggcgagtg agtattttaa tgaacaaaac gtccggcacg agacataaat
    14521 tcttcttcgc tcatctcctg tccgttaaac acaactttac ccggtgtata acgcagctgc
    14581 agcgaggcgg tattgttgtc aatcgtgatt aactgaaaca tctgacccaa tgtcatcatc
    14641 ccgctgattt gtttatcagc ctgcttttgc gctttttccg catccattcc ttcagataaa
    14701 ttaagctgtt ttgcggtttc tgtgacgaca ttaagcggta acttcacatc aaaattgagc
    14761 aatttgatat ctttgtttgt ggatgatgaa gatttggctg ggtcggcaat actgatatcc
    14821 agattggcat ttagttcgcc gagtgcgttc ttccatctta ccggttgttt aatggtcggc
    14881 tcactttttt gtaataacgg taaatactct ttgaacaatg cagcattcac ttcttgcaga
    14941 gcaacttcat cgtttgctaa ctcagggtgt acagcaagct gcttctgcat agcaatgtta
    15001 tactggatga taaattggcg cactgctgag ggatcaatag attcagcaat caatgaccac
    15061 tcaccactac cgagttgctg attccctttt gttaacttat caagttcgta ggtcagattg
    15121 acattgatat aatctttgtc tttaacgcga tccagcatgg tttttgcaac gaaagcatcg
    15181 atttgggcaa cgtcttcccc gtgattgata atgttaatct gattaaattt cgactcgctt
    15241 tcaccaaccg gaaatttctt ttcttctaat cgcgccagag aatcaatggt aaaacttttt
    15301 gccgttactt tgttcattgg tgataattgc agaacaatat cttcgacttt gccttcaata
    15361 tccaggttag acaatgaact accatcacca ttaaaagtaa actggccctc accaaaagaa
    15421 aactttccat actctgcagg ggcaacatct aatgtcgtag agaatgagcc accaaacgcg
    15481 aatcgcaatg tcgcttccac gggcgattta tttttcgcgg cgataaatag cggttgtgtc
    15541 agttcattat tcactaagtt cacttttgcc gctgccagtg ctgggatgac attaccatgc
    15601 attaacattg tgatgggcaa cggaccgtgc tcaacatcca catcaaatac aactttctgg
    15661 cctggcttga tattgagatc gggtgcaccg ttatcgaatg tcatttgcat ttgaaaacga
    15721 gaattaaaaa aacctttgtc aaaattttta taacttaacg tcatatcgta ggcatgttca
    15781 cctttctttt tagcatcgtt aaaatcttta ataaactttt cgacaccagg ctgaatttgc
    15841 gtacctgtgt accatgtgcc accaccccag attacggcta acgcaacaat aacacctgta
    15901 gctgacttac gtatcatata aatattccct gataaaacag caaaacttta aatatccgct
    15961 tatgctgaaa taatgtccag cggcaggacg cattatcaga atgtatttat atttattaaa
    16021 taataaaaaa aaccgtgaat attcacgg
//
LOCUS       DAZPLI010000087        15964 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZPLI010000087 DAZPLI010000000
VERSION     DAZPLI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15964
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.088"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..1848)
                     /gene="traD"
                     /locus_tag="ACHUU3_002669"
     CDS             complement(<1..1848)
                     /gene="traD"
                     /locus_tag="ACHUU3_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFR2082692.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLI
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGDPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQA"
     gene            complement(1899..2636)
                     /locus_tag="ACHUU3_002670"
     CDS             complement(1899..2636)
                     /locus_tag="ACHUU3_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2082693.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILEKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVLCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQGENITWSLSSQS
                     PADKYDLINKTSRVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRAAGGYHHRHTGYTWSVSVN"
     gene            complement(2839..3570)
                     /gene="traT"
                     /locus_tag="ACHUU3_002671"
     CDS             complement(2839..3570)
                     /gene="traT"
                     /locus_tag="ACHUU3_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFR2082694.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSSSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(3602..4099)
                     /locus_tag="ACHUU3_002672"
     CDS             complement(3602..4099)
                     /locus_tag="ACHUU3_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein"
                     /protein_id="HFR2082695.1"
                     /translation="MIMRCLAHIALVTVIQFIACYLVGWGKAETVFMLFFIVLWQGLF
                     IWLFSQIRKKRNVSDEFKFSKGVWYITMPVSSLLSPLLSLMVFIIGTLYELRRVSGCI
                     SIKKWGQNQLKNQYDGSEKLDFGGIEQPPTTYYNPSTGYPMHGGFDSAGNTFGTRWQD
                     YYDRQ"
     gene            complement(4118..6937)
                     /gene="traG"
                     /locus_tag="ACHUU3_002673"
     CDS             complement(4118..6937)
                     /gene="traG"
                     /locus_tag="ACHUU3_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFR2082696.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVQVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASGDPYTLIFSRPSPLRGVYDSNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TMNALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNSLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRSTRASAGMY
                     GDAHAEWGVKPKILGVGGGLGVRGGGRAGIDWSDDDAHQASSGSRASNDARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSV
                     QERMPGADSPQELMKRAKEYQDKYK"
     gene            complement(6934..8307)
                     /gene="traH"
                     /locus_tag="ACHUU3_002674"
     CDS             complement(6934..8307)
                     /gene="traH"
                     /locus_tag="ACHUU3_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFR2082697.1"
                     /translation="MLRIKPLFILCAALLTAIPTASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNI
                     NIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEGG
                     TAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGF
                     ISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYD
                     EAVIEHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(8294..8686)
                     /gene="trbF"
                     /locus_tag="ACHUU3_002675"
     CDS             complement(8294..8686)
                     /gene="trbF"
                     /locus_tag="ACHUU3_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFR2082698.1"
                     /translation="MTENKKNQEFKIRKIKRNIERSCDNAIKYFELFVVFFVAGLLLW
                     HGMHAFFSAGIDSWKADPELNNLIYMWNILMYAIPYTLYALAAGFLVTSFLSPMYELI
                     FGNIMIFLLKRRMRRENTLREGSNNASH"
     gene            complement(8640..8954)
                     /gene="trbJ"
                     /locus_tag="ACHUU3_002676"
     CDS             complement(8640..8954)
                     /gene="trbJ"
                     /locus_tag="ACHUU3_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFR2082699.1"
                     /translation="MRNKQVVLLIAGISGVATGIIVSLNIPFIRQGLFYPASPAEIVV
                     SLCLTFSVSVVFFVGAIVGWISVSEIYYSCMACLNESSEISEETYDDRKQKESGIQNQ
                     KN"
     gene            complement(8944..9489)
                     /gene="trbB"
                     /locus_tag="ACHUU3_002677"
     CDS             complement(8944..9489)
                     /gene="trbB"
                     /locus_tag="ACHUU3_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFR2082700.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQMYGEEKGAK"
     gene            complement(9476..9760)
                     /gene="traQ"
                     /locus_tag="ACHUU3_002678"
     CDS             complement(9476..9760)
                     /gene="traQ"
                     /locus_tag="ACHUU3_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFR2082701.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            9841..10176
                     /locus_tag="ACHUU3_002679"
     CDS             9841..10176
                     /locus_tag="ACHUU3_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFR2082702.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSLPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYVYFCELFRSHWIAVWFILWPVVINLTIGFGIYDK
                     LAPVISLMK"
     gene            complement(10157..10498)
                     /gene="trbA"
                     /locus_tag="ACHUU3_002680"
     CDS             complement(10157..10498)
                     /gene="trbA"
                     /locus_tag="ACHUU3_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFR2082703.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSAIWSG
                     VMWFFIISLVK"
     gene            complement(10512..11255)
                     /gene="traF"
                     /locus_tag="ACHUU3_002681"
     CDS             complement(10512..11255)
                     /gene="traF"
                     /locus_tag="ACHUU3_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFR2082704.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLVHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(11248..11505)
                     /locus_tag="ACHUU3_002682"
     CDS             complement(11248..11505)
                     /locus_tag="ACHUU3_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFR2082705.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTAGEML
                     AACFVIFYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(11532..13340)
                     /gene="traN"
                     /locus_tag="ACHUU3_002683"
     CDS             complement(11532..13340)
                     /gene="traN"
                     /locus_tag="ACHUU3_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFR2082706.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRTYLQK
                     SVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFRRHTAMK
                     LRLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQSC
                     WAYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGK
                     VMVCGGDVFCLDGECDKAQSGKSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQA
                     KFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGV
                     CLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGGAKHPDCRGITVDELQKIQFDRLDF
                     TNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(13337..13975)
                     /gene="trbC"
                     /locus_tag="ACHUU3_002684"
     CDS             complement(13337..13975)
                     /gene="trbC"
                     /locus_tag="ACHUU3_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFR2082707.1"
                     /translation="MKLSMKSLAALLMMLNGAVMASENVNTPENRQFLKQQENLSRQL
                     REKSDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(13984..14976)
                     /gene="traU"
                     /locus_tag="ACHUU3_002685"
     CDS             complement(13984..14976)
                     /gene="traU"
                     /locus_tag="ACHUU3_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFR2082708.1"
                     /translation="MKRSLWLLMLFLLAGHVPSASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKIPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(14973..15605)
                     /gene="traW"
                     /locus_tag="ACHUU3_002686"
     CDS             complement(14973..15605)
                     /gene="traW"
                     /locus_tag="ACHUU3_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFR2082709.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKALDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEESRK"
     gene            complement(15602..>15964)
                     /gene="trbI"
                     /locus_tag="ACHUU3_002687"
     CDS             complement(15602..>15964)
                     /gene="trbI"
                     /locus_tag="ACHUU3_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFR2082710.1"
                     /translation="DVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNAPVTAAFNM
                     KQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVSPAVVQGAP
                     DITREIQQDIARRMRAEP"
BASE COUNT         3837 a         4371 c         3723 g         4033 t
ORIGIN      
        1 agcctgagtc acatcttctc cggaggcaac ctccggttca aagaggctgg ccatctgacg
       61 accttctgct tcccttgcgg caagtacggc actcagacgg ttctccattt ccgggttgat
      121 gtcacgcgga ataaactccg gagcgacctt cggtcgtgcc tgatatttca gagagagttt
      181 gactgccgga tacggtccgg gcagagtgac atagcaggtc agatccggca gagactgaat
      241 gtcggaataa ctgaccagcg tctgacgctc catatcctta ccggtcgata ccccgtcacg
      301 taccgggtca gcaccgtagg aatactgctc gctggctttc aggtgctctt tctcaccaat
      361 ctcaccggca gcgaactctg caatcttatg gctcggggaa cggaaaaaag cacgggtgtt
      421 catgacgtca aacagcgtgg ccgccgcttt ctcaccgtag atatcttcca gctgggcata
      481 ggactggata ccaaacacat aacagccacc gaacttacgg gcttccggca ggatctccac
      541 cagatccggc agtttgtgta acgtgggtaa ctcatcacag aaaaaccaca cacgacggtt
      601 acggttttct cccattgcca gcagaccacg aatggcaatg gacagccaca tggagatcac
      661 cggcttcagg gaggcatggg tgtcggcgtt cgacgaaata aacagccagc cgtttttctg
      721 atcttcccgg acaccacgca tccagtcacg gatggtgaag ggatcaccgt tatgctcaat
      781 cccctgcagg taacggatgg ctttcacgta gttggtcagc acagcacgga tggaaatcgc
      841 cgttttctca attttctctt ccaccaggtt ggccgccggt gaattacgca ggaaggtacg
      901 cagtttttca attttgatgg aaagcagtgt gtccaccagt ttgctgtagc tgcggttggg
      961 gtcattacgc atcaggtacg ccgcttccgc aaaaatggta cgtcctgaac cctgccagaa
     1021 cgggtcctct tttgtaccca tagggatcag agtatttgcg gtattatcaa aatccggctg
     1081 tgtcaggcac tccttccaca aatcccatgc ggcacagcgg gcatccagcg gattcaggat
     1141 tttatcgatg gagggatcat agtaactttt aacaaattcc cctgaacggt cataaatcac
     1201 caccatatca ccgcgctgac gggcatagtt ggccagacga cggataacct ccgacttacc
     1261 ggccccgacc gtgccgtgca gacagaagtt ctggatttcg gaatcccgga taatcggcag
     1321 gtcgccaatc cggatatcgg agtccttgcc gtcttttttc agcatccggg caacgtcttt
     1381 cggattgtct gtcagctgac gaccacctgt gacttcattt tcgctctgtt gtttaccctg
     1441 acgccccaga atccaggaga caacaaagaa ggtgatcagg caaataacca gtgccacaac
     1501 tgtggccaga acgaatgcgg accatagctg ctctccgcac cagatcatat atttatcatg
     1561 cagtacctgg gcagcgttca tccggaaggt tttgccgtaa tactggatct catatactgg
     1621 ctgggacttg attaaatccc gcatgccttc cagcgtggta caccaccagt aaatacagcc
     1681 gttcacaaac gtctgccagc ttatttttac ccataaaatc agaccaacga gtatccagaa
     1741 aaaaataaac aggcagtaaa gcatgatatt ggcgatctgg ctgaacatgc ggatacgcat
     1801 ggacgcaatc tgaccgccct gggtcatatc ctttgcgtta aaactcatga tgaaactccg
     1861 ggaaaatctg gaacagggca gaaaaggatg atgtggggtt aattcacaga aacagaccag
     1921 gtgtaccctg tatgccggtg gtgatacccg cctgcagccc tgccggaaga gaccttctct
     1981 gtaacaatgt cagtcagtcg caatacttca gaccagtcac tcccgtgcgg aaactggatc
     2041 tgaataaaaa ggcgtacccg ttcacggtga tccagaactg tgtccggttc ataaaaacgg
     2101 ccactgtcag gagaaacacg ggaggtttta ttgatgagat catacttatc ggcaggagac
     2161 tgcgatgaca gagaccaggt aatattttcg ccctgatgac catcaatacg actgctcccc
     2221 ggcgtccatg caatttcctg cgcactcgcc acgatgatct ccctggagac ttctgccagc
     2281 tttctgggtg tacagagtac cggtgccagc acacacaggg acaacaaatt acagctgacc
     2341 gcctccggtt taagcaaatt gctcccggat ttttcaggaa aagatatttt ttccagtatt
     2401 tcagttaatt ccttatgcct gagagaatta ataaagtaca tgataagcat catgagaaac
     2461 aatataacaa taagccctcc gataaccact aaaaggtcag tatcaatatt attgctgtac
     2521 agatagtctt tagtggtcac tgtattatcc tgtatcaccg gggggtattc agttacaggc
     2581 ttctcagtaa attgattatt ctcaccaaca tattcagcga tattgtctga tgtcataatg
     2641 actttctccg gaagatcccg gcttcgccgg caacacttca cggtcgcaca aagaatttgc
     2701 acaaccgctc agtgttttca tatttatttc ggaaacgccg ggaggtgaca aaatttattt
     2761 tgttacatcc cggggttgga caacgagcaa cgcgaggcgt cagcaaggag gccggtcaga
     2821 tcagcctcct gacgaggttc agagaatatt tgcgattgat ttggccagct ggtcttcgag
     2881 aacaggcttc gcctcttcaa atttcaggtt aaccttgttc gcatttgaga ccacacgggt
     2941 ctggtattta tgctggttac ctgtttcagt actggtctga attttcgcac cggatgtgcc
     3001 ctgacgcagg gcagcaacat tatccgttgt caccgttgcc ttagtacgct ctgcaatctg
     3061 cacatccgtg atcatggtat agttcacatc ttccaccatc gcatctgcag ccatacccac
     3121 cagaccggca gcaaggccca caccgagtgt ggcaccggca gaactggaat tatatccggt
     3181 gataccggca cctaacgctg caccaactgc tgcgccttca taaccacggt tcagccatcc
     3241 ctgagactcc cgcagatcca tcttatcggc cttcagcaca ttcgcctgaa tccagtagta
     3301 ggctttatcc ggagaagtca ccacctgata tccttttgct ttcacagcat cagcaatttt
     3361 gccctgcagc ccactcatgt ctttatcaga cgtgtttttg atctgcagaa ataccgtgcg
     3421 ttcgctggcg ggttcaagcc agatggtctc actcatctga gtcttcacct caaggttacg
     3481 cttcttgatt gctgtgctca tcgcaccaca ccctgaaagg gccagagtgg aactgaccag
     3541 tgcaaccatc atcaattttt ttgttttcat caaattacct tttttacaaa ctacagtttt
     3601 cttattgcct gtcataataa tcctgccaac gagtcccaaa agtatttccc gctgagtcaa
     3661 agcctccatg catagggtat ccggttgaag ggttatagta ggtcgttggg ggctgttcaa
     3721 tgcccccgaa gtctaacttt tcactcccat catattggtt tttgagttga ttttgacccc
     3781 atttttttat gcttatgcaa ccactaactc gccttaattc gtatagtgta ccaatgataa
     3841 aaaccatcag gcttaataac ggagagagta aggaagatac tggcatcgtt atataccaaa
     3901 cacctttact aaacttaaac tcatccgaca cattccgttt ttttcgtatt tgagagaaca
     3961 accatataaa cagtccctgc catagcacta tgaagaacag cataaaaact gtttcggcct
     4021 tcccccaacc aaccagatag caggcgataa actgtatcac cgttaccaga gcaatatgag
     4081 ctaaacatct cattatcatc tcccaaacac agcattatta cttatactta tcctgatact
     4141 cctttgccct tttcatcagt tcctgtggac tgtcagcccc gggcattctt tcctgaaccg
     4201 acttttcttc attatattta ttattctgag ataatgcctc tgttttatgg tgattctgta
     4261 gttcagaata ctgtcctctt acaatatttt cctcgccacg tatgctgttc tgagtatcac
     4321 ctatattccc tttatattct gtgaccatat tatcaacctg atgttttaca tcattacgga
     4381 tattactgtc ctgcgttctt tgctcaataa tggcctgatg tccctgatgg tcagcaataa
     4441 tatcttgact gccaccaccc gaaggtacgc tctccattcc tttaccaata tcgccacggg
     4501 attcccgcca ggcattatca acaccaggct gtacctgttc ctgcacaaaa gaccaggcca
     4561 tcgcccgacg acgttctgca atctccggtg aactggtatt tgtcagaata gcttctgcat
     4621 cctgtggcgc gtgtttcatt acatactgtg caaactgttg cgacaagtct tcactcatct
     4681 gcccactcat gctttcagta cgggatgcca tttcagcata ttcatggctg cgggtcatat
     4741 tcgtcgtgta ctggtcgtaa ctctgcttcg ctgaattcag ggctgcagac aactgatcca
     4801 cacgggaatc agcattattg tcagtatggc tgccggactc actgaccttg cgactggtaa
     4861 agtaatcgct ggcctcttta aagtcttgtg tagctctggc atcgatatca tgacgagcat
     4921 cattgctggc ccgtgaacca ctgctggcct gatgcgcatc atcatcactc cagtcaatgc
     4981 cagcccgacc accgcctcta acgcctaatc ctccaccaac accaagaatt ttaggtttaa
     5041 ccccccattc agcatgagca tctccataca tcccggcaga agctctcgta cttctggatg
     5101 ccagttcctg agtcgcctgc tcattactga tattatgcgc cttcgcatag ctttccactg
     5161 cactgcgcat acggctggcc atcatggagt cctgtgcgct catcgtgctg tcagcaccac
     5221 ctgtgacaga atcactgctc ccccggttag aaccaaactg actgagcgag ttccaggcac
     5281 tggcaatact gctgctgaac ccatgcaggg cgctttctgc tctgttcgac gcctcccggg
     5341 ccatttcctg ttgtgccgcc gcaatctgac gcgttgcatt gatgccgacc ggtaaccggg
     5401 acatcgcccc gctcgcatcc atcaccatat taccgtcacg ggtctgtgtg gcagttgcgc
     5461 cactgccggt ctggtacatc atctgaccaa acgacgtggt gctgttggtg ctccagctga
     5521 agccgttcac gttttccgtc tgcatgttgc cgtaggagta attaccgtca accacactgc
     5581 ctgctgcact ggccgtcgga ctgatagagg aagaggcgaa gtggctgtac acgctggaaa
     5641 aacctgcgcc aaggcctttt accatcatcc atgacaacgg ggggatcatg gctgaaagat
     5701 acccggcagt agaggccagg tcagagtatt tcagctgtat ctgagagagt tcagacagca
     5761 cgaccggcgc accattcatc ttcgcataga atgtcatggc actgttcaga atggcataca
     5821 acaacggcca ggtctgcagc cacatcaggg caaacacata gcctttcagg acggacagtg
     5881 tcagtttgtt gaagacagct gccagtacca gaagcgggaa aataccaatg gcaattcccg
     5941 tcaggatagt ctgtaccatt ggcaggtttc gcattgtcac atgaccgata gagacatgcg
     6001 ccagacgttg cttctccatc gatgacgtgg tggccagatt caccaggcta gcggtgtcac
     6061 cattacgggc ggcattactg gtgataccct ctttcagggc attcatggtg acgttctggc
     6121 gcataatctg gctggcagac tgactggagc cgtagaaata actgtaactg tcactgacca
     6181 gttgtctgaa taacaggtcc gggtccggtc tgccgccaaa tatctgctgc acataataat
     6241 gccaggtctt gcctcccgtc tttgtatcga gattcagcct gtctttcagc gtgaccgacg
     6301 catctttaca ggttatgaaa ttattattgc tgtcataaac gccccgtagc gggctgggcc
     6361 gggaaaaaat cagcgtgtag gggtcaccgg aggccatcag gtcttccagc gtgtatttgt
     6421 ggttcaggta aatatccccc agcacgcagt tctggacgta gtcctgaaac agattgatga
     6481 tttccggatt ccgggacaga aaatcggtgc ttttcacaat caggttcgcc ccgaacagca
     6541 tgcctgtctt gctgtaggtg gcgctgtcag gctgggtgaa tatcatctcg taactggcca
     6601 ccattgcatg cccgatacgg gtcgtcagtg aaagtggcat cgccagtccg accggcacat
     6661 tatccacccg gtgaacctgg accaggtcac tgttatcaat aatctgcacc gatgtacgga
     6721 cattaaccag caggctgata agcacaaaca cggccaccca gcccagcaaa tccatcacgt
     6781 tgtgccgctg tacccacatc acggccaccg cgagaacaga caatgtgagc gcaatttttt
     6841 cgatggaatc ccacgtccgg gtccccataa aggcggcaat ggcgttcagg ttattgcgta
     6901 accactcacc accggcaatc acatagactt cattcacagc gtgctccctc cgaagtgata
     6961 gttgttctgg taacgactga gcatgcgggc ggaaagctgc tgacgcatgt agctcatctg
     7021 acgatcgaca accagcagcg catcctgctg tacctgcacc tgtgactgaa acgccgcaat
     7081 ctgccgggtg gcatcattca tgttgtcgtt aatatgctcg ataaccgctt cgtcataatt
     7141 tcccgtggcc accatcgccc gggcctgctg tatcagctcc tgaatgtact gcagcaggat
     7201 gtcgtaaccg atatagtccg tcagctggta aatcatgctg ttggaaacac cgagcatctg
     7261 cgggtcaacc agatatttga agacggggat ggtggtactg gaaataaagc ctttttcctt
     7321 gtcatccaga ggcgtgtcac tgaccgcctt gttctgaatg ctggccagaa gtttagtaat
     7381 ctgagacttc agtgcattat cccggctgat ggtcaccggt gtgtctgcca ccactttcag
     7441 gcatttatca gaatcgttgc agtgataaat ttttgctgtg ccgccttcca tcatggcccg
     7501 gataattgag cggtcagtgg ttctggctga caagggtgtg atttcgccgt taggaccaaa
     7561 caccagtgag ccggtcagcg tcatcacaaa ctctttcagc tctttgttgc cgtcaaacat
     7621 tctgtttttg gaaagcgcat tccacatgat gttgatgttt ttggtcaccc gctccttatc
     7681 cttgtcgctg gctttatccc tgacactgtc agatttcccg ccaacggtac atccctgccg
     7741 ggaagccgcc cagtcagcaa aaatattgct ctcaccggca atgtcctgac agactttctg
     7801 ctgggacacc tgcgtccggg ggaagagccc gccgataatc ccctgtgccg cctggcagga
     7861 actgaggttc atactgttaa tgtcgcttgc cattttctgc aggaagtctt ttgcggtttt
     7921 gatttccggc accgttgtct gcagggcaag gtcaaaaaag taaccggcag cgttgctcat
     7981 aatctgctta acaaaacgct gcaactgatc gccattaata aaactgaacg agccgagata
     8041 ggcgtcgatg ccgccacatc cggcattgat atccggcagc gtcatggaaa tcagctggat
     8101 atttttgacc tgggtgcggg catacaggga gccaccgtaa gcataaccgg cggcctgccc
     8161 ctgccagacg cccggctgtg tggtattgga ggcgaagccc agcttgttaa agaactgatt
     8221 catgtcgctg ttcacatccg ctgacgctgt gggtatggcc gtcagtaagg cggcgcaaag
     8281 aataaaaaga ggtttaatgc gaagcattat tgctgccctc ccttaatgtg ttctcccggc
     8341 gcatacggcg tttcagcagg aatatcatga tatttccgaa tattaattcg tacattgggg
     8401 acagaaaaga tgtaacaaga aacccggctg caagcgcata taacgtatac ggaatggcat
     8461 acatcaggat gttccacata taaatcaaat tatttaattc cgggtcagct ttccacgaat
     8521 caataccggc gctgaaaaaa gcatgcatac cgtgccataa aagcaatccg gcaacaaaga
     8581 aaaccacaaa aagctcgaag tattttatgg cgttatcaca actgcgctca atattccgtt
     8641 taatttttct gattttgaat tcctgattct ttttgttttc tgtcatcata agtttcctct
     8701 gatatttctg atgattcatt cagacaggcc atgcagctgt aatatatttc agatactgaa
     8761 atccatccca caattgcccc cacaaaaaac acaacagaaa cagaaaaggt aagacacagc
     8821 gaaacgacaa tttctgcagg gctggcggga taaaaaagtc cctgacggat aaaggggata
     8881 ttcaggctga cgattattcc cgtcgcgacg ccggatattc cggcaatgag taacactacc
     8941 tgcttatttc gcaccttttt cctctccgta catctgcaga acggtatcca tccgcgccat
     9001 aaaactggcg gcatccgttg ccccctgcaa aagcggtaat gcctcaagtg tgttgacgtt
     9061 gaccaggaag gtggtcggtg tggccaccgg aatattcggg aagaaggtct gcatcacgtc
     9121 cggtggcacc ggtaatgctt ccggaaaggc ggtatccccc tgaccatcca gggtgtaggg
     9181 aaaaacggaa aagccgtact gctgtgccag ctgtttcagt accgggtcaa actggtgaca
     9241 gtaagggcaa tgcccctgca taaacagcac caccttccag tcggccaggt taacctgcct
     9301 gccgttgctg aggcggaacc agcggggagc cggcttttct gttctggctg atgtgtctgc
     9361 tgccggttgt gcaggctgca cggccatacc ctgcggattc cagagtcgtt caatttcatc
     9421 ccgggtggag gcctgcacac ctgtggccat catcaacaaa atgaccggca gtaatttagt
     9481 gagagacatg tccgccctcc tgctgccctt ccatcatggc ctgttgcctg gcttccagtg
     9541 cctcacgctc ttccaggctg acacgtgcaa tccatgcatc cagcacctgg tatgcgccga
     9601 acagcacaag gatggccgca atcaactccg ccagaaaaaa agccatccag ggtttgctgt
     9661 aaacaagacg ggcgacgata tgaaaccaga cacccaggga aaatacccac attcccgtaa
     9721 tatcaagccg gggtaaagag aatctgcgtt tacttatcat gttaaactcc ggttattatt
     9781 tacatgagag tattcatgtc tgtaatattc gcgtttgtgt gatattaatc agaggtacaa
     9841 atgaaaaagt tatcttttaa agaaaaactg gaaatagtga gaaacatcat aagagagtca
     9901 ttatcaggta atgcggcttt tgctttactg gtgtattcat tattgcactc cctccccatt
     9961 aaaacctttg ctgatttatt tgtgacgata ctgttattta tagcgattat cgctgttgtg
    10021 atatggctat ttctaatcat ctatgtttat ttttgtgagt tattcagaag tcactggata
    10081 gcagtctggt ttatcctctg gcctgtggta atcaacctca ctattggatt tggcatttat
    10141 gacaaattag caccagtcat ttcactaatg aaataataaa aaaccacatc actccagacc
    10201 atatagctga caacataata caaacaaaca tccttctgaa actgtcaaac ttcgtgataa
    10261 ccggtatggt actgactgcc attgaaacaa ccggtacggc acactgattc ataaccgcac
    10321 cacaaagaaa caagaaatta tctggcagca tacatacact tatgacattt actacaaaga
    10381 agacaaacga gctcagcaga taaactcctt tattatttgg tcctgtaaag aaataaccaa
    10441 caacccccac gataaacagg ctggcacctg taaaaaaaca caaatcccct gtatgcatat
    10501 ataacctttg gttaaaaatt cggtttaaaa tcttcagaaa cgttcaggaa ctgttttgcc
    10561 aggtcgtcct gtgaaataaa gccgtatgat aacgggcgaa cactgccctg tttcgggtca
    10621 accagcatca tggccgggaa atatttcaca ccgaggcgct gcgcctgccc ctggtcagtc
    10681 cgggaatccg gcaacagcgg attaatcacg ccatccacgg aaacgggaat aacactcaga
    10741 ccatacgtat cccggaagcc attaatgacc tgcgccagct gcccgtcgat ggggtcctgc
    10801 ccccggtaaa aaaacatgat gccgtagtgt tcagccagtt tcgcaatggc ctgtcgctgc
    10861 tgcgcctggt ctgccgccag ctgattccgt accgtgccgt tgtaatggct gtactgcagg
    10921 ttatagtcca gttcgggatg taccagcatg gcctttttag cactcatggt gaaaagcccg
    10981 gcctgctgag tccagtaatt ctgcagccgg aaatatttaa caaaattatc cacaccggga
    11041 tacagaatgg cttcgtacag cgcccgcttt gttgccgtct gcagcgcggc cagtttctgc
    11101 ataatatccg gctcctgacg gggggctgca ggtacaggtt tattttcttt ttccttcgga
    11161 tttattttct cgttatacca ctgccagcct gcatctttcc ctgacgccgg aaaaataaag
    11221 cagcagagta acagtggcag taatgcttta ttcatcttca ttcctttcat cattttttct
    11281 ttcatcatct ttctgcaggc cagtcagggc agaaaaacaa aaatacagaa taaacaggag
    11341 cacaccggag taaaaaataa caaaacaagc agcaagcatc tcacctgcag tggtttcttt
    11401 taccgtatcc cacgaccaga taattaccgg ggaaatgaca atcgctgtaa tcaaaagccg
    11461 gataagaaaa tcaataaaac ggttcgacgt gaatatgact ttcatcattt tccccggtta
    11521 ttttccctac cttactgtcc tgcctgtttc agctggtcag caatctgctc tttcactttc
    11581 tgcgtcagaa caccactgtc cgggattttc tggttattca tcaggtcttc gtagaagttg
    11641 gtgaaatcca gtctgtcgaa ctgaattttc tgcagctcat caaccgtaat cccccgacag
    11701 tcaggatgct tcgcaccgcc aaaactgata cgcagctgtc cgttgcgccc ctgctgctgg
    11761 actatctgcg ccagcttcga atcaaactga cagtaactgc gttttttctc caggcagaca
    11821 cccagcactt tcttcgaaca gaactctccg acactgacgg tcagtttgtt tgattttgct
    11881 ttagccaggg ctttttcatc actgctgcat ttggccagcc cgatatcctg tccccagccg
    11941 ctgtccttac agcagttgct gtatccggct gcagccttct tacaaaattt cgcctgaccg
    12001 gtgaaagcac ggacatccac cccgttcagc gccgcaacat ccttacctgc tgcggccagt
    12061 gccgccagct gagaaacggc ttcggcaaaa tcattactct tcccgctctg agccttatca
    12121 cattcgccat cgagacagaa gacgtctccc ccacagacca tcactttccc ggatgttttt
    12181 gactcacagg agtacgtggc atattcatgc agacaggcac cttcttcgga gtaaaaggcg
    12241 cactgatgtg acaccagggt acaggcagga ttgtcggtgt aggtctgaca ggttcccttg
    12301 tctgctgact gtgtgacata cgtgtcccgg tatgcccagc agctctggta catgctgtat
    12361 gactgaccgt ctttcaccag cgtcctgttc ccgccagcct ccgtacattc tgtttttgtc
    12421 agtttcccca gctccttgct gaacgggcag ctttcagacc agacaacata cggggtgtac
    12481 gaggcttttt ttaccctgac aaccagacgc agcttcattg ccgtatgacg tctgaatttt
    12541 gtattcccga cccggacggg gtgagatgac gtaaaagcca caccggcagt cagtgttttc
    12601 tgtactggtg taaatgaggc atctgctgag tatttggcat tccagctcag cggggttccc
    12661 agtactgtaa ttgtcatagg tacactcttc tgcaggtaag tacgactcca gctgtaatgc
    12721 gcatcgacaa tttcaccggt aacgggagag gtaatggaga caacatactg accattcact
    12781 tcacgtgcag gtagctgact catttcatac tccagcgtcc gggtttccca tgtggtactc
    12841 ccctgcagct ccatacgggc agttcgtgtg cagtactgct ccacctgtaa atcccgctca
    12901 caggtgtaat tcgtgtattc actcctgcta atctcctgcg cactgcactg ctgcccggtg
    12961 tttcccacaa tgctgtcagc ccggttcacc acatccctgc cggtctggat aaacggtgca
    13021 tccggtgaaa gaatgtcttt cggcttgttc atgaatgact ctgtaatggt ttttccggtt
    13081 tcaccggtcg cccattccgt ggtgccgtca tttttcaggc cgccatcccc gccggcagtc
    13141 acgccaccgt agtattttgt ctcgtccgga ttcgcgttat agccggggat actctcctgt
    13201 ggcttaaagc cctgaatact gctgcttccc tgtcctttga tctggtgagc aaaatcagag
    13261 ccggcccggt aatcactgtt gctgtcagcc tgtgccatgc cggcaaccag agccagtatc
    13321 agaggtaaaa tacgtttcat ttcccggaat ctcctttccc tgccagtaaa tcatgagcca
    13381 cctgccggca gtcacccgtg gcggccactt tctccagcgc ctggccgacc cgcaggtttc
    13441 cccggatgat gtcgtatccc tgactgcaga acaccaccag tgccggtaca ctgcgaatgc
    13501 cgtactgtga aaacagcgtc gggtcgatct gaacaccatc agtcgcgccg tctttcacca
    13561 gggacaacac agcttcagcg gtggtcttca ggtcattgtt caccatgccc cgcagtgtgg
    13621 cgggtatacc gtagtgccgg gtttcgccca gcattcgttt cagcccctct tcgggaatgg
    13681 aaaacgacac aaaatacagg gcaccctgcc ggggtttccc gtcctgcgaa gcctgctgtt
    13741 tacgaaccag ctcatccagg aaatggttat ctgaacgctg aagggggttt tccagcacct
    13801 gcttctccgc ccaggctttc agctgatggt cagatttttc acgcagttgt ctgcttaaat
    13861 tttcctgctg cttcaggaac tggcggtttt caggagtgtt cacgttttct gacgccataa
    13921 ccgccccgtt cagcatcatc agcagtgctg ccagagattt catactcagc ttcatcattc
    13981 acctcacagg aagacacagt tacgtttacg ccacatcagg tagccgaagt ttttctttgt
    14041 attgggcgga tttttcccgg tttcccagcg ggtcacactg cgcccgaacg ggtggcactg
    14101 tccgctgtcc ggatacatat tcaccatctg ataacgccag cgttctttgg gcaggattgg
    14161 ggacggatat tcattacaga cggcattatt tttcccgatg gtttccataa tcatgccctg
    14221 acggtgcagt ttgaacgcca tgcgttcact gaccagcagg gaggactgca acggactgga
    14281 ctcattactc acccagccat tgaacggata catgctgccc tgtgaaccgg cacaccagaa
    14341 cagaacatcg agaggcatat taaaggcgct ggcaatggca tctgctgcac aggccccctg
    14401 tgctatcgga ttggcaaaga tgacagcttc cggattgaga atggtggtca ggctgctgtc
    14461 cgtccaggtg gggtcaattt cagaaagata agcgatatcc aggtcaccac cttccagaca
    14521 gcccagcgat gtgatgatgt tcagccagta cgtcagcggg tatttgtacc agtgaacgtg
    14581 atagaacgcc ccgttcacct gtttctcatc ctttttcgcc gttccctgtg ccgttttacc
    14641 aaaagccggc aggctgaagc ccaggttaac catgcatccc ggtgaccggg tgacgtccgt
    14701 cagcgccatc ggctcccagt aaccaatggc cagcccgata cgcctgaaca gcggcggcgg
    14761 tgccggacaa atctgaatgg gcatcgacgg gttcgccgtg tcggggatct taccctgact
    14821 gactttgata ctgcccagcg agagcgggaa aatacagctc cagcagatat ccgtgatcgg
    14881 gtttacaaaa cgcccctcac aggcagaatc cgcagacgcc gaaggaacat gaccggcgag
    14941 aaggaataac atcagcagcc acaggcttct cttcattttc tgctctcctc tgccggaata
    15001 aattccacct tcaggaagcg atcgccggga acggcgctca cccgtgccgg gacctgatcg
    15061 atgccgaggc gctggcagag cacaccgttc tggtcaaagt aaacacggct gtccagcgct
    15121 ttctgcatct ccgggatact tccctgcacc aggataattt tgctctccag tgtgggcggc
    15181 gtctggcgtt tcatccaggc cacctgcgcc ggatcatcgc cattgatgaa atacagcgtc
    15241 tggttaaaag gcacatactg cagcggattc atcacttcac cctgacgggc aaacacccgc
    15301 ccttcgttat cccggatatc cgcagccagt ctgacggacg ggtcaaacaa ccggctgctg
    15361 tatttctccg tgcgtccgat accgggcacg gcagggggcc gcagactgtt gcggatcacc
    15421 cgctccttaa aggcatccat tttccggccc atctcaccgg actgctccag tgccgtcagg
    15481 cgctgcatga tcaccgtcag catgtccggc tcctttaccg gccacagatc gccccaggtg
    15541 ccaagatcgg cggcggccac actctgcccc catatcagca gggcaatcag cccccggcat
    15601 ctcatggttc cgccctcatt cgccgggcaa tatcctgctg gatttcccgg gtgatatccg
    15661 gagccccctg taccacggca ggcgacacca gaatgaccgc gtggtgtttc tgctgccacg
    15721 cctgcaggct ggcttccagt gccgtgttaa aacgcgctga aagggctttt gactgtgctt
    15781 ctgacagttg tttctgactg gcgctgtcaa aaaacgcatc cacggtctgt ttcatgttga
    15841 aggcggcagt caccggtgcg ttcagtctga caatgccgta actgacggct gcgttcagta
    15901 acaccattgc caggcatccc ggcaccgtcc accaccagtg gctgcgtcgg ggggctgtaa
    15961 catc
//
LOCUS       DAZPLI010000088        15961 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZPLI010000088 DAZPLI010000000
VERSION     DAZPLI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.089"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHUU3_002688"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHUU3_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR2082711.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
     gene            complement(783..1751)
                     /gene="astE"
                     /locus_tag="ACHUU3_002689"
     CDS             complement(783..1751)
                     /gene="astE"
                     /locus_tag="ACHUU3_002689"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR2082712.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1744..3087)
                     /gene="astB"
                     /locus_tag="ACHUU3_002690"
     CDS             complement(1744..3087)
                     /gene="astB"
                     /locus_tag="ACHUU3_002690"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFR2082713.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3084..4562)
                     /gene="astD"
                     /locus_tag="ACHUU3_002691"
                     /pseudo
     CDS             complement(3084..4562)
                     /gene="astD"
                     /locus_tag="ACHUU3_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4559..5593)
                     /gene="astA"
                     /locus_tag="ACHUU3_002692"
     CDS             complement(4559..5593)
                     /gene="astA"
                     /locus_tag="ACHUU3_002692"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR2082714.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5590..6810)
                     /gene="astC"
                     /locus_tag="ACHUU3_002693"
     CDS             complement(5590..6810)
                     /gene="astC"
                     /locus_tag="ACHUU3_002693"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFR2082715.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7256..8062
                     /gene="xthA"
                     /locus_tag="ACHUU3_002694"
     CDS             7256..8062
                     /gene="xthA"
                     /locus_tag="ACHUU3_002694"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR2082716.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8229..8939
                     /locus_tag="ACHUU3_002695"
     CDS             8229..8939
                     /locus_tag="ACHUU3_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR2082717.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8944..9621
                     /gene="ydjY"
                     /locus_tag="ACHUU3_002696"
     CDS             8944..9621
                     /gene="ydjY"
                     /locus_tag="ACHUU3_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR2082718.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9636..10343
                     /locus_tag="ACHUU3_002697"
     CDS             9636..10343
                     /locus_tag="ACHUU3_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR2082719.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10343..10891
                     /locus_tag="ACHUU3_002698"
     CDS             10343..10891
                     /locus_tag="ACHUU3_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR2082720.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10901..12067
                     /locus_tag="ACHUU3_002699"
     CDS             10901..12067
                     /locus_tag="ACHUU3_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR2082721.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12040..13576
                     /locus_tag="ACHUU3_002700"
                     /pseudo
     CDS             12040..13576
                     /locus_tag="ACHUU3_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter permease"
     gene            13576..14229
                     /locus_tag="ACHUU3_002701"
     CDS             13576..14229
                     /locus_tag="ACHUU3_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR2082722.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14296..15603
                     /gene="ynjE"
                     /locus_tag="ACHUU3_002702"
     CDS             14296..15603
                     /gene="ynjE"
                     /locus_tag="ACHUU3_002702"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFR2082723.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRYDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15612..>15961)
                     /locus_tag="ACHUU3_002703"
     CDS             complement(15612..>15961)
                     /locus_tag="ACHUU3_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR2082724.1"
                     /translation="FYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAAK
                     HQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRV
                     WSGYLTLKSLQRQ"
BASE COUNT         3699 a         4343 c         4164 g         3755 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
      721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
      781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
      841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
      901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
      961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
     1021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
     1081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc
     1141 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
     1201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
     1261 cgttgcggta atacaccgaa ctgaggatgc aaggagccac gaattgcggt atgtagatca
     1321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
     1381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
     1441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg atccccgagg
     1501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
     1561 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
     1621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
     1681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
     1741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
     1801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
     1861 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
     1921 cgtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatactac
     1981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac
     2041 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
     2101 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
     2161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
     2221 aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta actgtgactg
     2281 gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg caatcacgtc
     2341 attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
     2401 gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg
     2461 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
     2521 cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
     2581 cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa aaatagcttt
     2641 taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc
     2701 aacggtgaga tgcactttgc catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
     2761 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgtgc
     2821 aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat
     2881 aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggtttt
     2941 cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa aacggtgacg
     3001 ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc
     3061 gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
     3121 ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag
     3181 taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat
     3241 ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc cacccgcgcc
     3301 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg
     3361 aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc
     3421 aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc
     3481 cccggcgtca gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg
     3541 cccatcgctt ccagttgctg ctaggcagta accacctgct gtgcggcctg ttcagaaatc
     3601 agcccgccaa taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg
     3661 acggcaacca gacgagcaag aaacgcatcg ccctgtgctc cgcttttgag caataaacgg
     3721 cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca
     3781 gccgagtcga tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg
     3841 gcgagaattt tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg
     3901 ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
     3961 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
     4021 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
     4081 gccagcaatg ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca
     4141 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca
     4201 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
     4261 atcgccgtca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
     4321 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga
     4381 cgttcagcca atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc
     4441 tgttcgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
     4501 ttacgcttca cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc
     4561 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt
     4621 tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg
     4681 ccagcaccac gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc
     4741 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc
     4801 ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac
     4861 gaaaaccttc tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga
     4921 tgacgtcctg ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
     4981 ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc gacgcggcta aaatccatcg
     5041 aaaagaagcg tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc
     5101 cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa
     5161 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
     5221 gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt
     5281 tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc gacccgatag ttgtaccagg
     5341 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacggtg cctgtctcgc
     5401 tatcttccag cacgaacaca tagccctgct cgcttttggg cagttcgcct tgccaggttt
     5461 tgattgccct ttcgatacgc gcggaaagcg tggcttcatt ggcgggaagc gacgtcaggc
     5521 cgccgcccgt tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac
     5581 ggatgaccat catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc
     5641 cagtccggtc gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt
     5701 gccacccgca atcagtacca taacgcctgc tttcgccgcc tcctgagaga tctgtttcgc
     5761 ttgtccggcg taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc
     5821 actgaacaaa ccgtagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
     5881 ctgtttaacg ccattaagca tctctggtgt gttgatgagc tccagcactt tgcccgccac
     5941 cgccgaggcc agcggattgc cgccataggt ggtgccatga gtgccaacgg tcatcacgct
     6001 ggcgcactct tcggttgtta acaacgcacc gaccgggaaa ccgccgccca gcgctttggc
     6061 ggtagttaac agatcaggtg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
     6121 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
     6181 acgcagacct tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg
     6241 ttcgacaatc accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt
     6301 atatgcagca tgacggatat ctggtggcag tggcgcaaaa tcctgtgaat aggctggctg
     6361 cccacccgca ctgacagtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
     6421 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
     6481 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat
     6541 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
     6601 cttactcgcc tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt
     6661 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga
     6721 accttcgcca cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc
     6781 aaagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgctatc
     6841 tatatgttaa caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
     6901 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
     6961 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
     7021 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
     7081 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
     7141 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     7201 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     7261 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
     7321 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     7381 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     7441 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     7501 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
     7561 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     7621 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
     7681 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
     7741 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
     7801 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
     7861 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7921 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7981 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
     8041 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     8101 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     8161 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     8221 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
     8281 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     8341 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     8401 ccaccctctt tgtgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
     8461 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     8521 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
     8581 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
     8641 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct
     8701 tctggcctta tacccttatt tcggcactca cgaccctgcc cggtattgtt atttataccg
     8761 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
     8821 tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
     8881 atgtggatct gtctgcttcg cgccgcagcc cgcttactca cccaaaaaat gaaggataga
     8941 acgatgtcgc aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg
     9001 gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac
     9061 gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg
     9121 ttggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
     9181 gatggctcca acggacataa atcgctgttt atggcttatg cgaccccgaa agcattttat
     9241 gaagccctga aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga taataaagaa
     9301 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
     9361 gcgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt
     9421 ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc
     9481 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggtgga aaaacgcggc
     9541 gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt
     9601 acctttaaaa tcaccgaata aagccaggat aaaggatgat gaagatgcaa tcgcgtaaaa
     9661 tctggtacta ccgtataacc ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc
     9721 tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc
     9781 aacagggtat agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct
     9841 tgttgatgat tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca
     9901 atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg
     9961 gcgcggcgct gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa
    10021 ccggcaaaac cgtgcttgac agtatggacg gctttttcac tcgctacggc aaacacacca
    10081 tcctggtctg tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccgatc
    10141 tgacttcaat acgttttcgc tcgtttttta tcgccaccgg gcttggtcaa ttaccgacga
    10201 ctattgttta ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg
    10261 gactgtttat tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg
    10321 agcgccagaa gaggaatgac tgatgggttt accgccgctt agcaaaattc ctttcatttt
    10381 acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg
    10441 gtgggggcgg atcccgttta tcttttattt ggtgtcaatg tttgttgggt ggctggagcg
    10501 caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat
    10561 gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa gtcgccgagc gtaccggcag
    10621 cagcgataaa ctgctggcag tggctgactg gcggcaaagc ccgctcttta gcgatgaaga
    10681 acggctggcg ctggagtacg ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc
    10741 cctgcgtacc cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt
    10801 gatcggcctg caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg
    10861 gctgtgccgt attccggaaa aacgttctta aggagagaag atgcgccatt gtgggtggtt
    10921 gctgggattg ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaattaa
    10981 aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa
    11041 ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat
    11101 tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg
    11161 tcgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt
    11221 aaaagaggcc aatttattac aaacgggctg ggcggagact ctgcccaact ggcgctatgt
    11281 cgacacacag ctgccggtgc gggaagattt ttcagtgccg acacaaggtg cggaatcgcc
    11341 ctggggcggc gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca
    11401 aacgccgcaa gccttactgg agtttgctaa agccaatccc ggcacggtta cttatccgcg
    11461 cccaccggac tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgccaga
    11521 tcccgccgca ttaaaagaag cgccggacga tgcgactttc gcccgtgtca ctgcgccctt
    11581 gtggcaatat cttgatgcac tgcatccgta tttgtggcgc gaaggaaagg atttcccgcc
    11641 ttcgcccgcg cggatggatg ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt
    11701 taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat ttgccggcaa gcagttacag
    11761 ttttggcttt cgcgagggga tgattggcaa cgtgcatttc gtcaccattc cagccaacgc
    11821 gaatgccagt gctgcggcga aggtagttgc caatttcctg ctctcacccg atgcgcaact
    11881 gcgtaaagca gatcccgttg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc
    11941 tgacgggcag cgcgaaatat tgcaatcaag aatgccgcag gatctgccgc cggtactggc
    12001 tgaaccgcac gcaggatggg tgaatgcact ggaacaagaa tggttacgcc gttacggtac
    12061 gcattaatct ttttgctgtg ggcgatggtg gcggtgattt atgcaccgct gatcccggca
    12121 gctctcacgc tgatatcgcc tgcgttgtcg ttgacacact ggcaggcgtt atttgccgat
    12181 ccacagttac cgcatgcatt actggcaacg ctggtatcga caaccatcgc ggcggtcggg
    12241 gcattgttga ttgccctgct ggtgattgtg gcgctatggc ctgggccgaa atggcagcgt
    12301 atgtgcgccc gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc
    12361 ctgctgctct ttgctgacgg agggctgcta tatgactatt tcccgtattt cactccgcca
    12421 atggaccgat ttggcatcgg tctgggcctc acccttgcag tgaaagaaag cgcatttctg
    12481 ctgtggatct tagcggcagt attgagcgaa aaacggctgt tgcagcaggt cattgtgctg
    12541 gattcgctgg gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg
    12601 ccttcgctgg caatggcgat gctggcgatt gttgcctggt cgctttcggt cgtggatgtg
    12661 gcgattattc ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta
    12721 actcagggcg acgcggatca acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg
    12781 ttactcgccg cctacgtttt gctgagctat ctgctatggc gcagctggcg gcgcactatt
    12841 ccccgcgtag atggcattcg caatgcctgc cacaccttta ttaccaggca ctacgctggc
    12901 gagtttttta cccttaaccg gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca
    12961 gtcgacgatc aatagtgaag cactcatcaa cagtctgaca atggggctgg tagcaacatt
    13021 catcgctttg ctcctgttac tgctgtggct ggaatggggg ccacagcgtc gccagttgtg
    13081 gctatggttg cccattctat tgcccgatct gccactggtg gcgggccagt acacgctggc
    13141 gctatggctg aacctagatg gaagctggac ggcggtggtc tgggggcatc tgctgtgggt
    13201 aatgccgtgg atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat
    13261 tttgattgcg caaacactgg gctggtcgcg ggccaaaatc ttcttttacg tgaaatgccc
    13321 actcatgttg cgccctgtgc tgattgcctt cgcggtggga ttttcagtca gtattgcgca
    13381 gtatatgccg acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaggcggt
    13441 ggcattaagc agcggcggca gcaacggtat tctcgccgcc caggctttat ggcaactgtt
    13501 gttaccgctt attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag
    13561 acaaggactc cgctaatgct ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc
    13621 ttgctgacaa acgttaactt tacggttgat aaaggtgaca tcgtcacgtt aatggggccg
    13681 tctggctgtg gaaaatccac tctgttttca tggatgattg gtgcactggc tggacagttt
    13741 tcttgtacag gtgagctatg gctcaatgag caacggattg acatgctacc caccgcacag
    13801 cgtcagattg gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa
    13861 aatttactgc tggcgctact gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa
    13921 gacgcgcttg atcgagcggg actggctgaa acttatcacc aggaccctgc gacattatca
    13981 ggcggtcagc gagcgcgggt ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta
    14041 ctcctggatg agccattcag ccgtcttgat gtggctctgc gcgataattt tcgccagtgg
    14101 gtgttcagcg aagttcgcga actggcgatc cccgtcgttc aggtaacgca cgatctccag
    14161 gatgttcctg ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa
    14221 ctgcgataac gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca
    14281 atgtcgggat tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg
    14341 ctggcttgcg cttcttcgtg ggccgctgaa ctggcgaagc ctcttacact tgaccagctt
    14401 caacaacaaa atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca
    14461 caaaccttaa atggcccttc tggtcatgaa cctgccgcct taaacctctc tgccagctgg
    14521 ctcgacaaaa tgagcaccga acagctcaac gcgtggatca agcaacataa cctgaaagcc
    14581 gatgccccgg tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg
    14641 caaaaagcag gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt
    14701 ctacaaaaac tgccgcactt tgagcagctg gtttatccgc aatggctgca cgacctgcaa
    14761 caaggtaaag aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg
    14821 ggcgctccta agctttacct tatcagccat attcccggtg ctgactacat cgataccaac
    14881 gaagtggaaa gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg
    14941 gcaaaacacg gcattcgcta tgacaccacg gtcattctgt acgggcgtga cgtatacgct
    15001 ggagcgcgtg tggcgcaaat tatgctctac gcaggcgtga aagatgtgcg tctgctggat
    15061 ggcggctggc aaacctggtc cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa
    15121 gtgaaagcgg aaccggattt cggcgtgaag atcccggcac aaccgcagtt gatgctcgat
    15181 atggaacagg cccgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg
    15241 tggccagaat ttatcggtac gaccagcggt tacagctata ttaaaccaaa aggtgaaata
    15301 gccggagcac gttggggaca cgctggtagc gactcgacgc atatggaaga ttttcataac
    15361 ccggatggca ccatgcgcag cgccgatgat attaccgcta tgtggaaagc gtggaatatc
    15421 aaaccagagc agcaagtttc attctactgc ggcaccggct ggcgcgcgtc cgaaaccttt
    15481 atgtacgcac gcgccatggg ttggaagaat gtttccgtgt atgacggcgg ctggtacgaa
    15541 tggagcagcg atccaaaaaa tccggtagca accggtgaac gcggcccgga cagtagcaaa
    15601 taacattgca attactgacg ctggagcgac ttcagcgtca gatacccact ccacacccgc
    15661 gtaaaggtcg tcatccagca caacgcaccg aagatccacg caaaccacgc aaaccatgca
    15721 gggaataaac atcccagcac aaacagtaag atcgtttctg tgccttcggt taatcccccc
    15781 agatagtaaa acgacttatg tgcataaccg gggttatcaa tctgatgttt tgctgccagt
    15841 gcagcaaagg cgagaaaact gctgcccgtg ccgataaacg caaacaacaa ccagccgcct
    15901 gccagcgcat tttgctccgg tgcagcaaga ataaagccaa acggcaccag cgcgtaaaag
    15961 a
//
LOCUS       DAZPLI010000089        15576 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZPLI010000089 DAZPLI010000000
VERSION     DAZPLI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15576
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.090"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            46..144
                     /locus_tag="ACHUU3_002704"
     CDS             46..144
                     /locus_tag="ACHUU3_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR2082725.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            147..221
                     /gene="yoaJ"
                     /locus_tag="ACHUU3_002705"
     CDS             147..221
                     /gene="yoaJ"
                     /locus_tag="ACHUU3_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR2082726.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(280..528)
                     /locus_tag="ACHUU3_002706"
     CDS             complement(280..528)
                     /locus_tag="ACHUU3_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR2082727.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(676..858)
                     /locus_tag="ACHUU3_002707"
     CDS             complement(676..858)
                     /locus_tag="ACHUU3_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFR2082728.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(862..1221)
                     /gene="yeaR"
                     /locus_tag="ACHUU3_002708"
     CDS             complement(862..1221)
                     /gene="yeaR"
                     /locus_tag="ACHUU3_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFR2082729.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(1394..2032)
                     /gene="leuE"
                     /locus_tag="ACHUU3_002709"
     CDS             complement(1394..2032)
                     /gene="leuE"
                     /locus_tag="ACHUU3_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFR2082730.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(2159..3082)
                     /gene="dmlR"
                     /locus_tag="ACHUU3_002710"
     CDS             complement(2159..3082)
                     /gene="dmlR"
                     /locus_tag="ACHUU3_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFR2082731.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNKGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            3185..4270
                     /gene="dmlA"
                     /locus_tag="ACHUU3_002711"
     CDS             3185..4270
                     /gene="dmlA"
                     /locus_tag="ACHUU3_002711"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFR2082732.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            4521..6131
                     /gene="yeaV"
                     /locus_tag="ACHUU3_002712"
     CDS             4521..6131
                     /gene="yeaV"
                     /locus_tag="ACHUU3_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFR2082733.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            6163..7287
                     /gene="yeaW"
                     /locus_tag="ACHUU3_002713"
     CDS             6163..7287
                     /gene="yeaW"
                     /locus_tag="ACHUU3_002713"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFR2082734.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            7343..8308
                     /gene="yeaX"
                     /locus_tag="ACHUU3_002714"
     CDS             7343..8308
                     /gene="yeaX"
                     /locus_tag="ACHUU3_002714"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR2082735.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGISITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8362..9477)
                     /gene="rnd"
                     /locus_tag="ACHUU3_002715"
     CDS             complement(8362..9477)
                     /gene="rnd"
                     /locus_tag="ACHUU3_002715"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR2082736.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(9559..11244)
                     /gene="fadD"
                     /locus_tag="ACHUU3_002716"
     CDS             complement(9559..11244)
                     /gene="fadD"
                     /locus_tag="ACHUU3_002716"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR2082737.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(11449..12030)
                     /gene="yeaY"
                     /locus_tag="ACHUU3_002717"
     CDS             complement(11449..12030)
                     /gene="yeaY"
                     /locus_tag="ACHUU3_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR2082738.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(12070..12765)
                     /gene="tsaB"
                     /locus_tag="ACHUU3_002718"
     CDS             complement(12070..12765)
                     /gene="tsaB"
                     /locus_tag="ACHUU3_002718"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR2082739.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(12823..14733)
                     /locus_tag="ACHUU3_002719"
     CDS             complement(12823..14733)
                     /locus_tag="ACHUU3_002719"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR2082740.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            14865..15209
                     /locus_tag="ACHUU3_002720"
     CDS             14865..15209
                     /locus_tag="ACHUU3_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR2082741.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         3831 a         4065 c         3787 g         3893 t
ORIGIN      
        1 gccacattgc tgaggcgtat tcacatcctt ttgattggtg ataacatgcg aatcggtatt
       61 atttttccgg ttgtaatctt tattacagcg gtcgtatttt tagcatggtt ttttattggc
      121 ggctatgctg ccccgggggc ataaagatga aaaaaacaac gattattatg atgggtgtgg
      181 cgattattgt cgtactcggc actgagctgg gatggtggta acgtcacctc taaaaaatag
      241 caaaggctgc ctgtgtgcag cctttgtgca atttaagcgt taacttttaa tcttcctgta
      301 gataaatagc acgacaatcg caccaataac ggcaaccacg aagctgccaa aattgaagcc
      361 atcgacttta ccaaagccaa acagcgtgct gatccatccg ccgactacgg caccgactat
      421 ccccagcagg atagtcataa agaatccacc accatcttta cctggcatga tccacttcgc
      481 cagaataccg gcaataagcc caaaaataat ccatgacaga atgcccattg tttcctcact
      541 tatctgtttt gcattagcgg gttagtcgct gataaaaagc atagcacaac atcgggaggg
      601 caagatttgt gacgagcatc acggaggatt tttttgcgat ggcgcagaaa ttgcgccatc
      661 aacgatcagt gataattacc aaccacaaac atcatgttcg ttttccgtgt cataagaacg
      721 cacggtattc accagatctt ttatcacttc agccgccact tctggcacca gcaaagtcat
      781 cggcgtctct gtttcataat cgacagaaac gccattactg ttattggtga cggtcacggt
      841 atacgttgct ttgcccatga ttcatttccc gttatgaatg actttccgtt gttgcgcacc
      901 ttccatcagg acttcaggag ccacgaagaa gtcaatgttg aaataagtat cgtcagtcat
      961 ggcttcaatg ttgtgccact tttctggagg gaacacctca aactgccccg cttcgataag
     1021 gatcacctga tcaggctctg cactgtgttc atcagcgtag ccgagatatt tgaccgcccc
     1081 atgcataacg gaaaggcgtg ggtaaacccc cgggcgcgtt cctttatcaa gatgacgttc
     1141 gaatattccg gcaggcgcag tttgtttatt ccagaaaggc gttgagcgcg tatgaatata
     1201 attctgtggg atttgcagca tccttttccc tccttcggtg aatgcgctga aaacggttta
     1261 ttccagccgt ttcagggtac gcctgataat ttgcatttta aataccattt attggttact
     1321 ttttagcacc atatcagaga aaaatcaggg aggattacaa gtgggaagcc cgcgacaacg
     1381 cgggctgaat gtatcaggat tgcagcgtcg ccagtcgggc ggcgaaaccc acgaacatca
     1441 aaccaatcag tgagttgcca actttagcca gtttcttttt ggtacgtatg tactgcgtga
     1501 caaaagcacc agaaataatc aggaagctca aatagcagaa actcaccagt tccagcgtcg
     1561 tcgccagaat aaagaatgaa attcccgtat gtggggcatt aacatcgata aactgtacga
     1621 aaaacgacac atagaacaaa atggctttcg gattagtcag gctcaaaatt aacgcgcgtt
     1681 taaaaatggc cccgtattgg ggctcatcgg atttggcctc gttattttta cccttcaggg
     1741 tcgcgtagag aattttactc cccagataga gcaaataaaa cgcaccaaga taacgtacaa
     1801 tgttgaataa tatcggggtg gtcttaatta atgtcgccac tccagcccat gccagaaaca
     1861 tcaatactgc atcgccaata aatacaccgc aggccgcaag ataaccgcct ttcataccgc
     1921 tactgacgct atttttgagt acaaacaggg tatttggccc tggcaccaac acaataaaaa
     1981 tggccccaac cagataggtc cagtaattca gaaccccgta ttcagcgaac acattaacct
     2041 ctttaattat ctttcgatca tgcgcgatta aaggtgaata tgctaaccaa tctgtagcgg
     2101 cttagaaagg agaaaatcag gttttaaccc gatatcaacc cgataattga ataattaatc
     2161 aggcatgctc cagtgaaaaa ttcgggtagt gctcggcaaa atactggcgt aaaaattcta
     2221 tcgttatccg cactttcgct gacgtcgcca gccttgaaac ataaacggcc cagacgttcg
     2281 ctggctggta atattccggt agcacttgca ctaaatgacc actggcaatg ttttcgctaa
     2341 catcccacca ggagcgcaga gcaatcccct gcccatcgag acaccactga tggacaattt
     2401 ctccatgatt agacgacagt gggccggtga ctttaatcgc atggtggcct tctttattgc
     2461 gtaattgcca gacaccaaaa ggatgatcgc gctctttaat gaccaggcac ggcaatgcgg
     2521 aaagatcggt taaatgtttc ggcgcgccat gttgcgcaat aaattccggc gacgcgcaga
     2581 gaatgcggta attagtcgcc agtttgcggg cgattaaatt aggggcgatg tcatcgccaa
     2641 tgcgaatatc gagatcaaca cctttattga ccaaatcgac caaccggtct tccacatcaa
     2701 aacgtaattc cagttgcgga tacgccttag ccagcgctga taacgccgga gccaccacct
     2761 gtcgtccaaa accaaagctg ctgataatac gcagcatccc ctgcggcacc tgacgcacgt
     2821 cagaaagttc gtccatcatc tgaccgacat cctgcaaaat ccgctgcgcc cattcataaa
     2881 tccgctctcc ttcttcggta atggtgacgc ggcgggtggt gcggtgtaac aacaccacgt
     2941 ttagcgtttg ctccagcaag gcgatgcgct tgctgacgaa cgccggtgaa acgcccagtt
     3001 cttccgccac ggcggcaaaa ccagcccggc gagccaccag cataaagacg cgcaaatcgt
     3061 tcagcagcgg taaattattc atgattcgtg ttttatgttt caccagttac ggggattaat
     3121 tccttttcag tcaattatag gatggtgatg ttgtcaattt tgatggtcag gaagtgagaa
     3181 cccaatgatg aaaacgatgc gtattgctgc gatcccggga gatgggattg gcaaagaagt
     3241 ccttcctgaa gggattcgcg tattacaggc tgccgctgag cgctggggct tcgacttgag
     3301 ttttgagcaa atggagtggg cgagctgcga gtattacagc catcacggta aaatgatgcc
     3361 ggacgactgg catgagcaac ttagccgttt cgacgccatc tattttggtg ccgtcggctg
     3421 gccggatacc gttccggacc atatttcgtt gtggggttcg ctgctgaaat ttcgtcgtga
     3481 attcgaccag tacgtcaacc tgcgcccggt tcgtctcttt cctggcgttc cctgcccgct
     3541 ggcgggaaaa cagcctggcg acatcgattt ttacgtggtc agggaaaaca ccgaaggcga
     3601 atattcctcg ctcggcggta gagtgaatga aggtacagag catgaagtcg tcattcagga
     3661 atcggtattt acgcgtcgtg gtgtcgatcg cattttgcgt tatgccttcg aacttgcgca
     3721 aagccgccca cgtaagacgc taacttctgc cactaaatca aacggtttag ccatcagcat
     3781 gccgtactgg gatgagcgag tggaagcaat ggccgagaat tacccggaga tccgctggga
     3841 caagcagcat attgatattc tctgcgcgcg ttttgtgatg cagccggaac gcttcgatgt
     3901 ggtggtggcg tccaatttgt ttggcgatat cctttccgat cttggcccgg cctgcaccgg
     3961 caccattggc attgccccat ccgccaacct gaatccggaa cgcactttcc cgtcgctctt
     4021 cgagcctgtc cacggttccg cgccggatat ctacgggaaa aatattgcta accctatcgc
     4081 cacaatttgg gccggtgcga tgatgctcga ttttctcggc aatggcgatg agcgtttcca
     4141 gcaagcgcat aacggtattc tggcagcgat tgaagaagtg attgctcacg ggccgaaaac
     4201 accggatatg aaaggcagtg ccaccacgcc acaggttgcc gacgcgattt gcaaaattat
     4261 tttgcgttaa ggtcaaacca gtttatttga accgcgtcac tgacgcggtt tttttattcg
     4321 ttctttgcag ttaataacct gcgtcatttc accttttatt gtttccgttt cgtgttttat
     4381 ggctttccgt attcttaatt gtttaattta tgtaacatgc aaataatcaa tttgttaaac
     4441 acaacataca actcattacc ctgcaaataa ttacaactgg caaaagccag agacgggctt
     4501 ttatgcccag gagatgactc atgatgagca atgttaagaa aaaagatgtg ccgctgataa
     4561 gcatcagcct ggtggccatt cttttcatcg cagctgcatt aagccttttc ccacaacaat
     4621 cggccgacgc ggccaacaca atatacactt ttgttacgcg tacgttaggt tccgccgtac
     4681 aggtattggt tttgctggca atgggactgg tgatttattt agccaccagt aaatacggca
     4741 atattcgtct tggcgaagga aaaccggaat acagcacgct ctcctggctg tttatgttta
     4801 tttgtgccgg tttaggttct tctacgcttt attggggggt tgctgaatgg gcctattatt
     4861 atcaaacgcc tggattaaat atcgcaccac gttcacaaca ggcactcgaa tttagcgtcc
     4921 cttactcctt cttccactgg ggcatcagca cctgggcaac ttatacgctg gcctcattaa
     4981 tcatggctta tcactttcat gtgcgtaaaa acaaaggtct gagtctttcc ggcattattg
     5041 ccgcgattac cggcgttcgc ccgcaaggcc catggggaaa actggttgat ttgatgtttc
     5101 tgatcgccac tgtcggcgca ctgactattt cccttgttgt caccgcagca acctttactc
     5161 gtggactttc cgcgctgacc ggtttacccg ataacttcac cgtgcaggca tttgtgatcc
     5221 tgctttccgg cggcattttt tgcctaagct cgtggattgg tatcaacaac ggtttgcaac
     5281 gtctgagcaa aatggttggc tggggcgcgt tcctgctgcc attactggtg ctgattgtcg
     5341 gtccaaccga atttattacc aacagcatca tcaatgccat cggcctgacc acgcaaaact
     5401 tcctgcaaat gagcttattc accgatccgc ttggcgatgg ttcatttacc cgcaactgga
     5461 ccgttttcta ctggctgtgg tggatctcat acacccctgg cgtagcaatg tttgtcaccc
     5521 gcgtttcccg cggtcgtaag attaaagaag ttatctgggg actgatcctc ggcagcaccg
     5581 tcggttgctg gttcttcttc ggcgtaatgg aaagctatgc cattcatcag tttatcaatg
     5641 gcgtaatcaa cgtcccacag gtgctggaaa cactgggcgg cgaaacagct gtacagcaag
     5701 ttctgatgtc gttgccagcc ggtaaattgt tcctcgccgc atacctgggc gtgatgatta
     5761 ttttccttgc ctcgcatatg gatgcagtgg cctacaccat ggcggcgacc agtacgcgta
     5821 atctccagga aggtgacgat cctgaccgtg ggctgcgtct tttctggtgc gtggtgatca
     5881 ctctgatccc gctttccatc ttgtttaccg gtgcttcgct ggaaacgatg aaaaccaccg
     5941 tcgtgctcac agcccttccc ttcctcgtca ttttactggt gaaagtcggc ggatttattc
     6001 gctggctgaa acaggattat gccgacattc cggctcatca agttgaacat tatctcccgc
     6061 agacaccggt tgaagccctg gaaaaaacac cagtgctccc tgcgggaacc gtattcaaag
     6121 gcgacaactg agcgccatca tcctaacgat aaaggtatcc ctatgagcaa tctgagccct
     6181 gactttgtac tacccgaaaa tttttgcgct aacccgcaag aggcgtggac cattcctgcc
     6241 cgtttttata ccgatcagaa cgcgtttgaa cacgaaaaag agaacgtctt cgccaaaagc
     6301 tggatttgcg tcgctcacag cagcgaactg gcgaatgcca atgattatgt gacgcgtgag
     6361 atcattggcg aaagcatcgt gctggtacgc ggtcgtgata aggttttgcg cgcgttctat
     6421 aacgtgtgtc cgcaccgtgg tcatcagttg ttgagcggtg aaggaaaagc aaaaaatgtg
     6481 attacctgcc catatcacgc atgggcattc aaactcgatg gcaatctggc ccatgcacgt
     6541 aactgcgaaa acgtcgccaa tttcgatagc gacaaagcgc aactggttcc ggtgcgtctg
     6601 gaagaatatg ccggattcgt cttcatcaac atggacccca acgccaccag cgtagaagat
     6661 cagttacccg gtctgggcgc gaaagtgctg gaagcctgcc cggaagtcca cgatctgaaa
     6721 ctggcggccc gctttaccac ccgcacgcct gccaactgga agaacattgt cgataactat
     6781 ctcgagtgct atcactgtgg tccggcgcat ccaggtttct ccgactccgt acaggttgat
     6841 cgctactggc acaccatgca cggtaactgg acgctgcaat acggtttcgc caaaccgtcc
     6901 gaacagtcgt ttaaatttga agagggtacg gatgcggcat tccacggttt ctggctgtgg
     6961 ccgtgcacga tgctgaacgt caccccgatc aaagggatga tgacggtcat ttatgaattc
     7021 ccggtggatt ctgaaactac cctgcaaaac tacgatattt acttcaccaa tgaagagtta
     7081 accgacgagc aaaaatcgct gattgagtgg tatcgcgatg tgttccgtcc ggaagattta
     7141 cgtctggttg aaagcgtaca gaaagggctg aaatcgcgtg gctatcgtgg tcaggggcgc
     7201 atcatggccg acagtagcgg tagtggcatt tccgaacatg gtatcgccca tttccataat
     7261 ctgctggcgc aggtgtttaa ggactaatga catcggcggc ggtattttcc gccgctgggc
     7321 tgatttttga tggagtacag caatgtcaga ctatcaaatg tttgaagtac aggtgagtca
     7381 ggtggaaccg cttaccgaac aggtgaaacg cttcacgctg gtggcaaccg atggcaaacc
     7441 gctgcctgcg ttcaccggag gaagtcacat cattgtgcag atgagcgatg gtgataacca
     7501 gtacagcaat gcgtattcac tactgagttc gccgcatgac acctcttgtt atcagattgc
     7561 cgttcggctg gaggaaaact cgcgcggcgg ttcccgcttt ttgcatcagc aggtaaaagt
     7621 gggcgatcgg ttaacgattt caacgcctaa taacctgttt gcgctaattc cctcagccag
     7681 aaagcatctg tttatcgcgg gcggtattag tatcacccct ttcctgtcgc acatggcaga
     7741 gttgcaacac agcgacgtcg actggcagct acattactgc tcgcgaaatc cagaaagttg
     7801 cgcatttcgt gatgagctag tccagcatcc gcaggctgag aaagtccatt tgcatcattc
     7861 atcaaccgga acacgactgg aattagcgcg attattggcg gatatcgaac ctggcacaca
     7921 cgtttatacc tgtggccccg aggcgctaaa tgaagcggta agaagtgaag ctgcgcgtct
     7981 ggacatcgcc gccgatacgc tgcactttga gcaatttgct atcgaagaca aaaccggcga
     8041 tgcctttacc ctggtgcttg cccgttccgg aaaagagttt gtggtgccgg aagagatgac
     8101 tattttgcag gttattgaaa ataataaagc cgcgaaagtg gaatgtttat gtcgtgaagg
     8161 ggtatgcgga acctgcgaaa cagcaatact ggaaggtgaa gctgaccatc gggatcagta
     8221 ttttagcgat gaagagcgtg ccagccagca aagtatgttg atctgttgtt cgcgtgcgaa
     8281 gggtaaacgc ctggtgttgg atttgtagtt tgccaaagcc ggatgtggcg ctgagcgcgc
     8341 ccagtccggc ttcggaagat tttactgcgg atattcctgc aataaattgt gtaatgcttc
     8401 cgccatcagc tcaccacgcc agccggaaat cagctccggc aaattgttct gcggtttcag
     8461 tttccagtgc cagttcagca gttggttgat ttgccgacgc gatgccagca attcggcgct
     8521 gatcttatgc gtttcgctca cgtcagtaat cagcgactta atcgctttaa acgctttacg
     8581 ataacccggc atgtccatca ggttaagcat cggctgcggt aaggcctctt ccggcaatgt
     8641 ctgcgctttt tccaccagcg ccagcagcgt tttaccgtga aagcggattt cgctaccgga
     8701 taaacccagg ctgtccagtt cgcctaaact tcccggcata taacgcgcga ccgaccacaa
     8761 atgctcttca cgcacgacaa agttcaccgc cagatcacgc tcacgagcct tgcgcagtcg
     8821 ccagtcggct aacagttgca gacaggccag ttggcgtgtg cgtaattgcc aggcattggt
     8881 gatatcacgc caggcatctt ccggcgcaac gacttcctga cgacgcattt gcatcaggcg
     8941 gcattcatcc agcgccgcag gtagccagcc ggaggcctcc gtttctacca taagcttggc
     9001 ggtgatcggt aacagatacc agacatccgc cgctgcgtat tcacactgac gttcggtcag
     9061 cggtctggcc agccagtcgg tgcgcgattc actcttgtcc agcgtaacgc cggaatactc
     9121 ttccaccatg gaagcgaaac cccatgacat cgggcgtccg cagaaggcag caaggatttg
     9181 cgtgtcaatc aggggttgtg gtaattcgcc aaagacattg aggaacactt ccagatcttc
     9241 actgcctgca tggagaaatt ttgtgatgga cggatcgcgc aggatcgctt tcagcggtga
     9301 ccagtcggtg atcccgagag gatcaattag cgccagatgc tcaccatcga aaagttgaat
     9361 caaccccagc tgcgggtaat aagtgcgcgt acgaacaaat tcagtatcct gggctatcgc
     9421 cggaaagaca cggacggctt cacacaaaga agccagcgca tcgtccgtgg taatcatttg
     9481 gtaattcaaa ttgtattctc tttagtgggc gtcaaaaaaa acgccggatt aaccggcgtc
     9541 tgacgactga cttaacgctc aggctttatt gtccactttg ccgcgcgctt cgtcacgtaa
     9601 ttctcgtcgc aaaatttttc cgacgttaga tttcggtaac tcatcacgaa actccaccag
     9661 cttcggtact ttgtagcccg tgagctgacg gcagcaaaag gtcaccagtg actcttcggt
     9721 aagcgatgga tcttttttca ctacgaagat tttcaccgct tcaccactgg agccggaagg
     9781 tacgccaaca gccgcgactt cctgtacgcc aggatgctgc atgacgacat cttcaatctc
     9841 gttgggatag acgttaaaac cggaaaccag aatcatgtct tttttacgat cgacaatgcg
     9901 caggaaccct tcttcatcca tcaccgcgat gtcgccggtg tgtaaccagc catttttgat
     9961 gatctcatcg gtagcatcgg gacgctgcca gtaacccagc atcacctgcg gtcctttgac
    10021 acaaagctca cccggttgac ccggtggtac ttcattatcg tcgtcatcca ccagtttggc
    10081 ttccgtcgac ggcaccggca aaccgatgct accactatga taatcaatat catatgggtt
    10141 aacgctgacc agcggcgcac actcggtaag accatagcct tccagcagat actgtcctgt
    10201 cagtttcacc caacgctctg ccaccacttg ctgcactggc atcccaccgc ctgcggaaag
    10261 atgcagactg gagaaatcca gctgctggaa ctctttattg ttcagcaacg cattgaacaa
    10321 ggtgttaacg ccagtgatag cggtaaacgg atatttcgct aactctttta ccaaccctgg
    10381 aatatcgcgc gggttagtga taagcaggtt ctgcccaccc agttcgataa acagcaggca
    10441 gttaatggtc agggcaaaaa tgtgatacag cggcagtgcc gttactacca gttctttacc
    10501 tggatgcagc aacggcccat aggtggcgtt gacctgttcc aggttcgcca gcatattgcg
    10561 gtgagtcagc atcgcgcctt tcgccacacc tgtggtgccg ccggtgtatt gcaggaaggc
    10621 taaatcttcc ggcaccagtt cgggtttgac gtactgcatc cggtagccgt tatgcagtgc
    10681 gctacgaaat gaaatggcat ctggcagatg gtatttcggc accaaacgct tgatgtattt
    10741 aacaacgaaa ttgactaccg tgccttttgc cgtagatagc tgatcgccca tacgggtcag
    10801 aattacgtgc tgaacggcgg ttttatcaac cactttttcc agtgtgtgag caaagttaga
    10861 cacgataaca atcgccgatg cgccgctatc gttaagctga tgctcaagct cacgcggggt
    10921 atacaacggg ttaacgttta cgacgatcat cccggcacgc aaaatgccaa acagcgccac
    10981 cggatattgc aataaattag gcatcatcaa cgcaacgcga tcgcctttct tcagccccaa
    11041 cccttgttgc aaataagcgg caaacgcgcg actgcgttct tccagcttgc ggaaggtcat
    11101 tacctccccc atattcacaa acgcaggctg atcggcgtag cggacgaccg actgctcaaa
    11161 catatctatc agagattgat aacggtcagg gttgatctcc gtcggaacat ccgcgggata
    11221 acggttaagc caaaccttct tcaattcttc acctctaaaa tgcgtgttcg tcgtcatcgc
    11281 aaccccaagt gataaacatg ttgttaacat aacattaact catcttacca gcttgataat
    11341 tagacaacga aaaggttgcg aagcgcgtca ctatttattt ttatagttgc cgtatgaacg
    11401 cagaaacagc ggaccagccg ctgtttcttt ttctttaaaa acaacttgtt actcggttac
    11461 aattgtttca actcttgcag ggccaggatt ataccaaccc catccgccgt aaccataggg
    11521 ccagccgcgt ccaccataaa accatggatc aattggctgc ggcggcataa tcacctgttg
    11581 agtgatatgc cagcgtttgt aacctgttac ctgcataacc ataaatttat atggtgcatt
    11641 atttactttg ccatccactg tgccagtgat tggcccgaca accgtcacca gttgtccacg
    11701 aaaatcgacc ggatcaagaa aaccgttcac atcggcataa atgcgcccgc gtgaaggttc
    11761 tcccaaggtt ggtcttgctc cgctgtccag cggtacggta gcaatttcca ggcgggtttt
    11821 cccttgctgg ttttgtacca caaccacttt gccgccaaag cgcgcctcct gaccaacata
    11881 taaatctggc gcattcatca cgcgtaccag atcttgctgt ggtgttggac tgctgccttt
    11941 aatggcgtcc ggcacagtga cacaaccgct cagcattagc gcaaacgtac ctgccagtat
    12001 gcctttgata acattctttt gaaccgccat gatgcgactc ctttttttct cagggcatac
    12061 tcttaagatt cattctttgc ccggaagttt cttccatgcg acgttgttac gtaaataaac
    12121 cggttcggca tgttccaccg ccaccgtttt accctcggca aacatctgac acgcaatcgg
    12181 cagcatatct tccgccgctg gcagtaaaac ttctccgtcg cgcaaaacca gcccgctctc
    12241 tttacggaga tccggccagg cttgccagcc cgtccctacc gttacccatt cgccggaaag
    12301 ctgttgcatt cgttcatgaa cgagttcggg tttgagtacg gcttcggttt cttcaccgcg
    12361 ccagataccg ttttcatcac gctgatattc ggcccagtaa acttcgccca ttcgcgcgtc
    12421 aatggctgcc agcacgcggg ttgcgccgtt tttgcgccac gccccctgcg ccatcgtcat
    12481 tagcgtagag acaccaatca tcggtaactc cgcgccaagc gccagccctt gcgcaatacc
    12541 aataccaatg cgcacgccag taaagctacc ggggccgcgc ccgtaagcca gagcgtttat
    12601 gtcagtcagg gaagttccgc tggtggtcag gatatcctgc accatcggta agattcgttg
    12661 agtatgttca cgagggcaaa gctcaaaatg agcgttgaca ttaccgtcgt tccacagagc
    12721 gacagagcag acctctgtcg cggtatcgat agccagaatt cgcatgggtc ttcgtgctta
    12781 gatcaataaa aaggcgcgca tcataccata ctccgtaaca aattacctgg aggatggtat
    12841 cgcaaggaaa cgaaccgcac gggcaatgtc acgggtgcgt ggcgcgggcg gcagactggc
    12901 gagaaacgtc gcgccgtaag gacgcatcac cagccgattg tcacaaatta ccagcacgcc
    12961 gcgatcgtcg gcgtcgcgaa tcagtcgccc tactccctgc ttgagagtaa tgaccgcatc
    13021 cggcagctgc acttcatcga acgggtcgcc accgcgcaaa cgacaatctt ccatgcgcgc
    13081 ttttaacagt agatcatccg gcgaggtaaa cggcaatttg tcgataatta ccaatgacaa
    13141 tgtatcgcca cgcacgtcca ccccttccca gaaactactg gttgccacaa gaagcgcgtt
    13201 accggcgctg acaaattgct gcaacagttg ccctttgctg gtttccccct gcaacaaaac
    13261 gggaagcgtc atggtagcgc ggaactgctc ggccagatcg cgcatcatgg catgcgaggt
    13321 acaaagcata aaacaacgac cgttgttagc ttcgatgatc ggtcgcagca ttgccgccag
    13381 ttgccgtgca caacccggtt gattggtttg cggcaggttg cgcggcacac agagtaacgc
    13441 ctggcggctg taatcaaacg ggcttggcaa tagcaacgac tccgcctgtt ctatcccaag
    13501 ccgcgaggtg aaatgatgca gatcgtcgtt caccgacagc gttgctgagg tgaagatcca
    13561 gctaccgggc ttttgcgcca ttaactcttt gaatttatcc gccacgctga gcggcgtgag
    13621 agccagagta aaatggcgcg aagtgcattc gtaccagtag ctgtagcccg gctgattgat
    13681 ctcttttagc cgcttcagcc gtgtgcgata caacgtggcg cgctcaaatg ccgcatccag
    13741 caaggcggaa cgccccagtg acagtttcgc cacgtcataa caaagttcca gggtgtcatc
    13801 gagcagtaaa aatgcccgct gaatttgcgg attagctaac agctcacgca ggttaccgcg
    13861 ataacctggc tcaccgagtt gcagacgaaa atcctgtgca ctctgggcaa gacgatcagc
    13921 gcacttttgt aactgctggg tgtcttttaa ttcggtgcgg taggcgatgg tgatgtcttt
    13981 tgccaggtcg agcagttgtc gactggagag tgactgacca aaatactggc tggcaatgtc
    14041 cggtagctgg tgggcttcgt cgaagatcat gacgtccgct tcagggatca actcgccaaa
    14101 tccactctct ttaaccacca tatccgccag aaagagatga tggtttacca ccaccacatc
    14161 ggcgtccatc gcttttttac gtgctttgac cacaaagcaa tctttataca tcgggcagtc
    14221 gctgccaaga cagttgtcgt tggtgctggt gaccagcggc cacgcctgtg aatcttccgc
    14281 cacgctgacg caggtgctga tatcaccatc gactgtttga ttagaccagg agcgcagcag
    14341 gatcacatcg cttaagattt gtaccggcag atcgcccccc gccagcgcct gctgttcgag
    14401 acgttcgagg cagaggtagt ttgagcgccc tttcagcagc gccacgttgc ccgtatattt
    14461 caatgccttt gagactgttg gcaaatcgcg gctgtagagc tgatcctgca acgcttttga
    14521 gccggtcgag ataatgactt tctttttcgc ccgcagcgca ggagccaggt aagcgtaggt
    14581 tttgcccgta ccggttcctg cttccaccac cagcggctgg cctttttcta tcgcctgggt
    14641 gacggctacc gccatctgtc gctgtggttc tcgcggctta aagcctggta tcgctttcgc
    14701 cagctgaccg tctggtgcaa aatcgtccgt cacactaccc cctgttgatt tgaacaggga
    14761 ttatgtcagg atgagggcgc tttcgccagt tgaagtggtg acggcaacct cacattgtgg
    14821 cagtctttgc agcacgaaaa tggaagtata atgaggacaa aatgatgact atcgttcgta
    14881 tcgatgctga agcccgctgg tctgatgtag taatccacaa caacacgctc tactacactg
    14941 gtgtaccgga aaacctcgac gccgatgcct ttgagcaaac cgccaacacg ctggcacaga
    15001 ttgacgccgt gctggaaaaa cagggcagca ataaatcgag cattctggat gccaccattt
    15061 tcctggccga taaaaacgac ttcgcggcga tgaataaagc gtgggatgcc tgggttgtcg
    15121 cgggtcatgc gccagtgcgc tgcacggtac aagcgggttt gatgaacccg aagtataaag
    15181 ttgaaattaa gattgtggct gcggtgtaag ctttatcgaa gcaaaataag tcagacgata
    15241 atttatcgat aatactggtc ggttttacat aaatcgacca gagaataaga tattactgtt
    15301 gatgcttctg ctaattcatt atttatattt ataatttcaa ttttatctat aaaactaact
    15361 atataaataa acataaccat gccgttgtgt tcgccctttg attagcaact ctggttttct
    15421 ttcttagctg aacaatctgg ttatagcgtg cggactgacc gttaattttc tccttcttac
    15481 tggataacac accatgagct gctaccttta aaacacctga atgtcaacag gttaactcgt
    15541 gccatatcgt tataatccct ttgatattag atgcaa
//
LOCUS       DAZPLI010000090        15511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZPLI010000090 DAZPLI010000000
VERSION     DAZPLI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.091"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            202..474
                     /gene="asr"
                     /locus_tag="ACHUU3_002721"
     CDS             202..474
                     /gene="asr"
                     /locus_tag="ACHUU3_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR2082742.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            567..650
                     /locus_tag="ACHUU3_002722"
     CDS             567..650
                     /locus_tag="ACHUU3_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2082743.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            750..1571
                     /locus_tag="ACHUU3_002723"
     CDS             750..1571
                     /locus_tag="ACHUU3_002723"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR2082744.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHUU3_002724"
     CDS             complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHUU3_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR2082745.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHUU3_002725"
     CDS             complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHUU3_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR2082746.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHUU3_002726"
     CDS             2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHUU3_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR2082747.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHUU3_002727"
     CDS             complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHUU3_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR2082748.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHUU3_002728"
     CDS             complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHUU3_002728"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR2082749.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHUU3_002729"
     CDS             7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHUU3_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR2082750.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8347..9729
                     /locus_tag="ACHUU3_002730"
     CDS             8347..9729
                     /locus_tag="ACHUU3_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR2082751.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9766..10488
                     /gene="folM"
                     /locus_tag="ACHUU3_002731"
     CDS             9766..10488
                     /gene="folM"
                     /locus_tag="ACHUU3_002731"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR2082752.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10485..10820)
                     /locus_tag="ACHUU3_002732"
     CDS             complement(10485..10820)
                     /locus_tag="ACHUU3_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR2082753.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10949..11668
                     /gene="rstA"
                     /locus_tag="ACHUU3_002733"
     CDS             10949..11668
                     /gene="rstA"
                     /locus_tag="ACHUU3_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR2082754.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11672..12973
                     /gene="rstB"
                     /locus_tag="ACHUU3_002734"
     CDS             11672..12973
                     /gene="rstB"
                     /locus_tag="ACHUU3_002734"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR2082755.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13049..13978
                     /gene="tus"
                     /locus_tag="ACHUU3_002735"
     CDS             13049..13978
                     /gene="tus"
                     /locus_tag="ACHUU3_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR2082756.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHUU3_002736"
     CDS             complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHUU3_002736"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR2082757.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
BASE COUNT         3812 a         4021 c         3793 g         3885 t
ORIGIN      
        1 ggttatcgct cgccacggga atatctgcgg cagcgggctt gtaatgggtt aagtgataac
       61 agatgtctgg aaatataggg gcaaatccac tgccattccc agggatatag ttatttcaac
      121 ggccccgcgg tggggttaaa tgaaaaaaca aattgagggt atgacaatga aaaagtatta
      181 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
      241 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat
      301 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
      361 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
      421 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
      481 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc gggtaacgtt
      541 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
      601 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
      661 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
      721 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
      781 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
      841 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
      901 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
      961 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acacctccaa
     1021 agggtaaagc cgataaagca gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct
     1081 atgagatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
     1141 atggggatgg ttggattgta cctcccgcag ccgcgccgtg ggacttcgga ttgattgtgc
     1201 tacgtaatcc cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc
     1261 ttactgccgc attaaaatcg gctggccgta aagtaactca ggcgggatac cctgaagatc
     1321 atctcgatac gttgtacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
     1381 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata
     1441 ccaatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
     1501 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
     1561 cgcaaaaata atgttcaaac gctgcccgac tgtgcgggca gcgtgttcat caggcaagtt
     1621 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
     1681 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
     1741 atgcataagc gacagacaag tcgatccctt taacggcctg agaaagcgca ctaaaggcag
     1801 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
     1861 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
     1921 cccattcaaa ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct
     1981 gattttatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
     2041 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
     2101 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
     2161 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
     2221 catttcattg acagcgtacc ggtaatttct gtagcaatag ccaggcctaa taaaatccaa
     2281 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
     2341 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
     2401 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
     2461 ggtaaatgtt gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
     2521 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
     2581 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
     2641 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
     2701 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
     2761 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
     2821 cattatttat tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgagtgc
     2881 cgcgtgtact ggcggtgtcg attttgatga ccatcatcgt gatggcgatg gtgttgctat
     2941 tagcttatct gggttccgcg ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact
     3001 ctattatgac gccgctgcaa gctcttgagc cgttgttgca acgcgtaggg attgacgtct
     3061 cagttgacca gctggcgcat tacattgatc cgaacgcggc gatgacgttg ctcaccaact
     3121 tattgacgca gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt
     3181 ttatgctgct cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg
     3241 ttgaagggat ggcggcgatt caacgtgcaa ttgacagtgt ttctcattat ctggtgctga
     3301 aaacagccat cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg
     3361 ttcgcttcgc ttttgtctgg ggattgctgg cctttgcgct taattacatc cctaatattg
     3421 gttcagttct cgcggcaatc ccccctatcg ctcaggtact ggtgtttaat ggcttctacg
     3481 aagcgttgct ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg
     3541 agccgcgcat catgggacgt gggctggggc tttccacatt ggtggtattt ttgtcgttga
     3601 ttttttgggg atggttgtta ggaccggtgg gtatgctgct ttccgtgccg ttaacaatta
     3661 ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg
     3721 atctcaataa agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt
     3781 gcatccacgc tggctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc
     3841 gggttttgca caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc
     3901 gctttccaca cttccagcac aggcatacca gcaataggac tcttcggatc atcctgcgcc
     3961 gccgggttaa ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca
     4021 tcgttgatct cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat
     4081 acgttcatat gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata
     4141 ccacgagcgc gcagtttctc ggtaatttca gcgacaggat attgcgcctg cgcgactgcc
     4201 atgccgtacc ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct
     4261 tctgcggtga tttcgcggtg ctcgcccact tcctgatcat cgccagtaga agagccgtcg
     4321 gtgccgaaac cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg
     4381 taagaaagga tagcccccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg
     4441 ctgagcataa agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc
     4501 accactggca tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca
     4561 atggcggtca ttattagcaa tgccagcact tgcaggccga cgctgtccgt gcgaacaaat
     4621 acaatcagca gcaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc
     4681 agcatcaacg gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgaaccc
     4741 gtgaacgtta ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc
     4801 agaatcggtg ccattcccgc gtcatgatgc agatagctgt taaagccaac cagcactgcc
     4861 gccagaccca cgaagctgtg caggatcgcc accagttctg gcatttcggt catttcaact
     4921 ttcttcgcca gacggatacc aattgcccca ccaatgacca tcgccagcaa gatccagcca
     4981 acattacccg tatccggtcc aaaaatggtt gcgattaacg caatcgccat cccggcgata
     5041 ccgaagttgt taccctggcg agacgtttca tgtttcgaaa gaccggccag actgaagata
     5101 aacaggatcg cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc
     5161 ttaatttttg cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt
     5221 aatgctggct ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat
     5281 ctgcaacagt gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg
     5341 tgtatgcagc gcgtgcgata cattccacac cacgtaataa ccgacaacgc aggccagcgc
     5401 gaaaacggtg aagtgcccaa gaaactcttt cggcgcaacg cttgccagcc aaccgaaaag
     5461 gatgattgcc agcgccatca acgcgtattt acgccacggt gagcaggcac atttttcctc
     5521 agttttcact tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg
     5581 cggtgccggc caggtaattt cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc
     5641 atcaaaatca acagtgatat tgccgtcttt ctctttgcac aacagtttca gcagattaac
     5701 gaggttagta ccgtaaagct gcgaggattg cgtcggcaga cggcccggaa gatcggtata
     5761 accaatcact ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca
     5821 gttgccaccg ttttgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac
     5881 catttcacgg gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat
     5941 gatatcgacc tcttttgcct gggcggcaaa gagttccatt tccgctttaa tgaacgcgtc
     6001 cgacatcact ttggcatagc catcgccgct gcccgcttcc tctttaaaat ccagctcgag
     6061 gaattccgcg cccatacttt gaacttgttc tttcacttcc ggacgggtgt cgaatgcacg
     6121 cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc
     6181 aatcaccatc acttttgccg gtggcacttt cccggccgca gtaatttgcc cggtaaagaa
     6241 gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga
     6301 gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc acagaatcca tcgccatcac
     6361 ggtcacgtta cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa
     6421 actcaccagc gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac
     6481 cttcagaatg atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg
     6541 cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt
     6601 aaaacccagt ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt
     6661 ggttaaccgt tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga
     6721 tgatggtttg cctgtgtcag gagccacaca agccgctcat gtacgagcta aatgttactc
     6781 cgttaaaata aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta
     6841 gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag
     6901 tgggataaac accttagaac gccggataaa gactgataat tgtcttcgac ggtcgggtaa
     6961 aacgagacat cgccccggca cgattcacta cttaacatta aattaactta tacaattcag
     7021 ttgcttcagt agtaatgatg ctgatacggc tgttttttaa gcatagacgg tcatttgagc
     7081 aggattaaaa ttggcttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt
     7141 aaatgcaata atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag
     7201 gcgaaggatt atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc
     7261 tacggcattc tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa
     7321 accttttgac cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc
     7381 cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga
     7441 ttttggtaac agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa
     7501 agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc
     7561 tgttctgatt gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga
     7621 agtcaatgat gccatcacca aagcggcaaa agcgaaaggt gcctactctt tctacatcgt
     7681 tcgtcaaatc gatgccaacc aaggcggcaa ccagcgtatt actgcattca tctataaaaa
     7741 agatgctaag aaacgtatcg tccagagccc ggatgtgatc ccggcagatt ccgaagcagg
     7801 acgtgcagct ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc taggtgttgc
     7861 gactaccgca tcaccaagtt ctgaagtcgg tcgtttcttt gaaacccagt catcaaaagg
     7921 cgggcgttac accgtcatgc tcccggatgg cactaaagtc gaagaactga acaaagcgac
     7981 cgcagcgatg atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac
     8041 cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat
     8101 cacccgccag tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgtataaata
     8161 actattcagt tactcctata aggcggcttg atgccgcctt tcgcattttt agtcacaaat
     8221 ttccccgcga ccattgcatg cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa
     8281 actccatttt tatgcgtttt gccctaatat ttattcttct tatcacgttt taatcactgg
     8341 atatcgatgg aaaagaaact gggactgagc gcactcaccg cgctggtatt aagctcaatg
     8401 ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg cagttgccag cccggcagca
     8461 ctgctcatcg gctggggtat tactggcgct ggcattttat tgctggcctt tgccatgctg
     8521 atcctcacgc gcattcgtcc cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg
     8581 tttggcgagc taatcggctt ttgttccgca tggggatact ggctgtgcgc agtcatcgcc
     8641 aacgtctcct atctggtgat cgttttttcc gcgttaagct tttttacgga cacgccggaa
     8701 ttgcgcctgt tcggcgatgg taatacctgg caatcgatcg tcggtgcatc agctttattg
     8761 tggattgttc actttttgat cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg
     8821 gcgacactgg caaaattgtt gccgctgggt ctgtttgttg tgctggcgat gatgatgttc
     8881 aaactggata ccttcaagct cgacttcacc ggacttgcac ttggcgtacc cgtttgggaa
     8941 caggtgaaaa acaccatgct gatcaccctg tgggttttca ttggtgtgga aggtgccgtg
     9001 gttgtttctg cgcgtgcgcg taataaacgt gatgttggca aagcgacact gctggcggtt
     9061 ctctccgctc tgggcgttta cttgctggta acgctgcttt cgctgggtgt ggtggcacgt
     9121 cctgaactgg ctgaaattcg taacccgtca atggccggtc tgatggtgga aatgatggga
     9181 ccatggggcg aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta
     9241 agctggacca tcatggcggc agaagttccg ttcctggctg ccactcataa agcattccca
     9301 cgcatttttg cgcgccagaa cgcacaagct gcgccatccg cctcactgtg gttgaccaac
     9361 atctgcgtgc aaatttgtct ggtactcatc tggctgaccg gttccgatta caacacgttg
     9421 ctgaccatcg cctcagaaat gattctggtg ccctatttcc tggtcggcgc gttcttgctg
     9481 aaaatcgcaa cccgtccact gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta
     9541 tggttattgt acgcctctgg tccaatgcac ttgctgttat ccgttgttct gtatgcgccg
     9601 gggctgctgg ttttcctgta tgcgcgcaaa acgcatacac atgataatgt actgaaccgt
     9661 caggaaatgg tgctgattgg catgctgtta attgcctcag ttccggcgac ctggatgctg
     9721 gtgggataac gttcatccca tcgttggata gcaaaggaga taacgatggg taaagcccag
     9781 cccttgccaa tattaattac tggcggaggt cgtcgcatcg gcctcgccct cgcctggcat
     9841 ttcattaatc aaaagcaacc ggtgattgtc agctatcgga cgcactatcc agccattgat
     9901 ggactgatta aggcaggtgc gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg
     9961 atggcgtttg ccgatgaagt actaaaaagc ccccatggtc tgcgtgctat tttgcataac
    10021 gccagtgcgt ggatggcgga aaaaccgggt gcgccactgg ccgacgtact ggcttgcatg
    10081 atgcagatcc acgttaatac cccatacctg ctcaaccatg cgctggaaag attactgcgt
    10141 gggcacggac acgccgccag cgatatcatt cactttaccg attatgtggt ggagcgcggt
    10201 agcgacaagc atattgcgta tgctgcaagc aaagcggcac tggataatat gacccgctcg
    10261 tttgcccgca agctggcacc ggaagtgaaa gtgaactcta ttgctccatc gctgatcctg
    10321 tttaatgaac atgatgatgc cgaatatcga caacaggcgc tgaataaatc actgatgaaa
    10381 accgcgcctg gcgagaaaga agtgatcgac ctggtcgatt acttacttac cagttgcttt
    10441 gtcaccggac gcagtttccc acttgatggc ggtcgtcatc tgcgttaatg cagctttatc
    10501 cagcatataa tcaacaccca tgcgcttatc ccccaacacg aaacagaacc aacaaaagcg
    10561 gcgggcaagc gcatcattcc ggtgaaatac cacagcgaca ccaggtaaac aaaataggga
    10621 ataatcgacc acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg
    10681 gcaacaatat aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata
    10741 taataatttt tcgtttttgc taaaacacca atcaacagca ctaccagcgc accgagcgcg
    10801 gctttgatta ccagccccat ttttttacct taacacttcc ataacaagtc atcagtagaa
    10861 tacctgatga aaacttgttt agaaacgatt gatagtaagt aaaaacagcg cggtgtattg
    10921 tgacgttttt atatctaccg tgaatgttat gaacactatc gtatttgtgg aagatgatgc
    10981 ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat gatatgcagg ttaccgtaga
    11041 gccgcgcggc gaccaggccg aagaaaccat tttgcgagaa aatccggatt tggtgttact
    11101 cgacatcatg ctaccaggca aggacggcat gaccatttgt cgtgatttac gcgcaaagtg
    11161 gtctggaccg attgttcttc taacctctct cgatagcgat atgaaccaca tcctggcact
    11221 ggaaatgggt gcctgcgact atattctcaa aacgacgccc cctgctgttt tgctagcgcg
    11281 tttacgtttg catttgcgtc agaatgagca agccacactg accaaaggtc ttcaggaaac
    11341 gtctctgact ccctacaaag ccctgcattt cggcacgttg accatcgatc ccatcaaccg
    11401 cgtagtcacc ctggctaaca ctgaaatctc gctctcgaca gctgatttcg aattattgtg
    11461 ggaattagct acccatgccg ggcaaatcat ggaccgcgat gcattgctga aaaatttacg
    11521 cggcgtcagt tatgacggac tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa
    11581 aaaactgctc gataacgccg cagaacctta tcgcattaaa actgtgcgta acaaaggcta
    11641 tctttttgcg cctcatgcat gggaataagc gatgaaaaaa ctgtttatcc agttttacct
    11701 gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt gggctggtgt acaaatttac
    11761 cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg aacagttcac tgtatctgat
    11821 gcgcagcgaa ttgcgtgaga tccccccaca cgactggggt aagacgctga aagagatgga
    11881 tttaaatctc tctttcgatc tgcgtgtcga gccactgagt aaataccatc ttgatgatat
    11941 ttccatgcac cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt acacgttttt
    12001 gcagcgtatc ccgcgcagcc actacgtgct ggcagttggt cctgttcctt atctttatta
    12061 tctccatcag atgcgattgc tggatatcgc cctgatcact tttattgcta tttccctcgc
    12121 ctttccggtg tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc
    12181 ggcgcaacga tttggcgatg ggcatctcaa tgaacgtatc cactttgatg agggttcgag
    12241 ctttgaacga cttggtatcg catttaacca gatggcggac aatatcaacg ccttgattgc
    12301 cagcaaaaaa cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct
    12361 gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg
    12421 tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg
    12481 cccacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct
    12541 ggcagacatt caggcagtaa cgcccgataa aacggtacgg attaaaacgc tcgcgcaagg
    12601 ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa
    12661 cgccctgcgg tactgccatt caacggttga aaccagcctg ctactgtcgg ggaatagagc
    12721 gacattaatt gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt
    12781 tgaacctttt gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg
    12841 gctggcaatt gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag
    12901 cgaactgggt ggtgctcgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt
    12961 tacctctgcc tgacactacg cgcacgatgg tcaagtcacc accactgtgc tataaaataa
    13021 gtatgttgta actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa
    13081 cactaccttt cgccagatgg aacaagagct ggctgcattt gccgctcatc ttgagcaaca
    13141 caagctattg gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa
    13201 tccgcttaat cgtattgagg taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt
    13261 gcgtcatttc cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc
    13321 tgtccgtctg cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt
    13381 cagtcatatt cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga
    13441 atcagaactc cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat
    13501 cacccttaat gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg
    13561 ttgggctaat aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga
    13621 aaaaagcctg aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa
    13681 actggagcga gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa
    13741 acgtccggtg aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt
    13801 ccaacacgcc tgccctacac cgctgattgc actgattaat cgggataatg gcgcgggcgt
    13861 gccggacgtt ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc
    13921 acaggcgcag ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cggattaacg
    13981 cccggctttc atactgccga ccatctgttc tggccgtacc cagctgtcaa actcggcttc
    14041 gctaagatac cccagcgcaa gggccgcagc ttttaaggtc agcccttctt tatgcgcttt
    14101 tttggcgatc tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat
    14161 cagcgattca ttgagtaatt gattgattcg ctcacgattc ggttcaatac ccactgcgca
    14221 gtgtttgtta aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattgtg
    14281 gatcaccatt ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat
    14341 cgccacgtcg ttccccatta cctgacagca gagcatggtt aatgcttcac actgggttgg
    14401 gttcactttc cccggcatga ttgagctgcc cggctcattt tccgggattg agatttcacc
    14461 aattccgcag cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga
    14521 cgcagccaac cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgcaagcgc
    14581 ttcaaatttg ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc
    14641 tacgcgacgc gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccgag
    14701 agccagttcc gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat
    14761 cgctacccag ccggaaatct cctgccccag tgttagcggc gtggcgtcct gcaagtgggt
    14821 tcgaccgatt ttgacgatat cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag
    14881 ggttttaacc tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc
    14941 cgtcggaaag acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt
    15001 acgttccatc ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt
    15061 catgttcatg ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc
    15121 gtcatgctgt cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc
    15181 agacaacaag cctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc
    15241 atgaatcagt gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg
    15301 agtttgtgcg ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc
    15361 gctgcgtact gtattcatgg cctgctcctc acctgattaa ttttttcttt ctgttttgcc
    15421 ttcgttaagc aacttttagc tcacttatta tttactattt gataacaaat gtttggtctt
    15481 tcgtgccatg taaaaaaacc gccccgaagg g
//
LOCUS       DAZPLI010000091        15499 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZPLI010000091 DAZPLI010000000
VERSION     DAZPLI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15499
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.092"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="ACHUU3_002737"
     CDS             complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="ACHUU3_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR2082758.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1598..4654
                     /locus_tag="ACHUU3_002738"
     CDS             1598..4654
                     /locus_tag="ACHUU3_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR2082759.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4651..5061
                     /gene="menI"
                     /locus_tag="ACHUU3_002739"
     CDS             4651..5061
                     /gene="menI"
                     /locus_tag="ACHUU3_002739"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR2082760.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5161..5349
                     /locus_tag="ACHUU3_002740"
     CDS             5161..5349
                     /locus_tag="ACHUU3_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR2082761.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            5897..6265
                     /gene="sufA"
                     /locus_tag="ACHUU3_002741"
     CDS             5897..6265
                     /gene="sufA"
                     /locus_tag="ACHUU3_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR2082762.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6274..7761
                     /gene="sufB"
                     /locus_tag="ACHUU3_002742"
     CDS             6274..7761
                     /gene="sufB"
                     /locus_tag="ACHUU3_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR2082763.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7771..8517
                     /gene="sufC"
                     /locus_tag="ACHUU3_002743"
     CDS             7771..8517
                     /gene="sufC"
                     /locus_tag="ACHUU3_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR2082764.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8492..9763
                     /gene="sufD"
                     /locus_tag="ACHUU3_002744"
     CDS             8492..9763
                     /gene="sufD"
                     /locus_tag="ACHUU3_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR2082765.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFASLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9760..10980
                     /gene="sufS"
                     /locus_tag="ACHUU3_002745"
     CDS             9760..10980
                     /gene="sufS"
                     /locus_tag="ACHUU3_002745"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR2082766.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            10993..11409
                     /gene="sufE"
                     /locus_tag="ACHUU3_002746"
     CDS             10993..11409
                     /gene="sufE"
                     /locus_tag="ACHUU3_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR2082767.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11558..12562
                     /gene="ldtE"
                     /locus_tag="ACHUU3_002747"
     CDS             11558..12562
                     /gene="ldtE"
                     /locus_tag="ACHUU3_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR2082768.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="ACHUU3_002748"
     CDS             complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="ACHUU3_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR2082769.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(13171..14583)
                     /gene="pykF"
                     /locus_tag="ACHUU3_002749"
     CDS             complement(13171..14583)
                     /gene="pykF"
                     /locus_tag="ACHUU3_002749"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR2082770.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(14591..14785)
                     /locus_tag="ACHUU3_002750"
     CDS             complement(14591..14785)
                     /locus_tag="ACHUU3_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2082771.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            15140..15349
                     /gene="fumD"
                     /locus_tag="ACHUU3_002751"
     CDS             15140..15349
                     /gene="fumD"
                     /locus_tag="ACHUU3_002751"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR2082772.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         3917 a         3744 c         4096 g         3742 t
ORIGIN      
        1 agctgaatgg cggtaagcag gaaaatatgg ggaagagcaa gcaaatatca attagcggtg
       61 attaacaatc aggcggcaga gccgcctgat gagaaattac ttattcggtt tctcgatttc
      121 gccaagttct tcgagaattt cttccggttg gtccgtcggc ggcgggactt cttccaccca
      181 cgcggcaaac agacgccagg aaacggctaa cagaaccgga ccaataaaca gaccgatcat
      241 cccgaaagca atcaaaccac caataacgcc agagaggatc aggatcagcg gtaaatcggc
      301 acccatgcga attaacattg ggcggatgac gttatccagc gtgccaacca caccgctcca
      361 cactaacaat accgttcccc aggtggtatc gccggtccag tagagccaga taatcgccgg
      421 aatcagtacc ggcaacgggc caagctggac aaggcaggag aggatcatta acaccgttag
      481 caacgttgca taaggtacgc cggatacggc gaggccgata ctgccaagca ctgcctgtac
      541 taacgccgtc accaccacac ccagcgccac cgcgcggata gcctgtgccg ccagcagcac
      601 ggcggcatct ccgcgaacgc ctgccagacg ggttgcaaaa tggcgaatgc cttgtgccac
      661 ctgttcaccg cgccagtaca gcagggcact gaagagaagc atcagcgcac aatgcaccat
      721 aaagcgcccg atatgcgccg cctgcccaac gaaccaggtg gtggtggtgc caatataagg
      781 gcggactttc gccatgatcg ccgtaccccc catatccagc aagttgtgcc agcctgcata
      841 cagcttcgcg ccaatcaccg gaatggtatt aagccacgcc agatcgggta acgtcatgtc
      901 gccgctggaa atggctttaa ttagcgggcc gctgccgtcg acgatactgt taaccagcaa
      961 agcaataggg atgataaaca ccatcactaa taacagcgtc atcaccagaa cggcgagaga
     1021 gcggcggcca aacatgatct tttgcaaacg taacaatacc ggccaggtgg cgataaccac
     1081 cgtaccggcc catgcaaagc cgagaataaa gggttgaaca atccacagac atgccacaat
     1141 catgatggct aaaaacagca ccgaaagcag aatttgtgcg acatccctgg gctgacgaac
     1201 atttaccata actacttttc accttttttg tgcgtcaaat cgtcgacgcg acgtgaacac
     1261 gcgaaactca cctgcataat gatgagcaat ttcagcggtt tttaacaggc cgattctgcc
     1321 cttaattctg atgggcgtgt tagaaaaaaa tgtggtacaa caactcaggc aacacgcaaa
     1381 cgatttactc gtcgtatttc aagttgcatg cgctgcggct acgttcgttc accccagtca
     1441 cttactattg taagctcctg gagttcactc gcttgccgcc ttcctgcaac tcgaaatcca
     1501 tagagtaaat aaacatcacc tttcagtgcg ctattgcagc cgcctgaaag aatagtgtat
     1561 cgacctacat cacagaccgc aggaaagggt caatataatg attccacaga tttcccaggc
     1621 acccggcgtc gttcaactgg tgcttaattt tttgcaagag ctggagcaac aaggttttac
     1681 cggcgatacg gcgacaagtt atgccgatcg tctgacaatg tcgaccgaca acagtattta
     1741 ccaacttctc cccgatgcgg tggtatttcc gcgttcaacc gcagatgtgg cgctgatcgc
     1801 ccgtcttgcc gcgcaggaac gctattcatc gctgatcttt accccccgcg gcggcggcac
     1861 cggcactaac ggtcaggcgc tcaaccaggg gattattgtt gatatgtccc gccatatgaa
     1921 ccgcatcatc gaaattaacc ctgaagaggg ctgggtgcgc gttgaggccg gggtgataaa
     1981 agatcaactc aatcagtacc tgaaaccgtt cggctacttt tttgcaccag aactttcgac
     2041 cagcaaccgg gcaacgctcg gcgggatgat caataccgat gcatccggtc agggatcgct
     2101 ggtctatggc aaaacgtcag atcacgtact tggcgtacgc gcggtgttgt tggggggcga
     2161 tattctcgat acgcaacctt tacccgtcga actggcggaa acgctgggta aaaccaatac
     2221 cacaatcggg cgaatttata acacggttta tcaacgttgc cgtctgcaac gccagttaat
     2281 catcgacaac ttccccaaac ttaaccgctt tcttaccggt tacgatctgc gtcatgtctt
     2341 taacgatgag atgaccgagt tcgacctgac gcgcattctg acgggttcag aagggacgct
     2401 ggcctttatt accgaagcgc ggctggatat tacgcccttg cctaaagtgc gccgtctggt
     2461 gaacgtcaaa tatgactctt ttgactccgc gctgcgtaac gcgccgttta tggttgaggc
     2521 gcgggcgctt tcggtagaga cggtggactc aaaagtgctg aatctggcgc gggaagatat
     2581 tgtctggcat tccgtcagcg agttgattac cgatgtgcct gacaaagaga tgctcgggct
     2641 gaacattgtg gaatttgctg gtgatgatga ggcgctgatt gacgagcgag ttaatgcact
     2701 ctgtgtgcgg cttgatgagc tgattgccag ccaacaggca ggtgtgattg gctggcaggt
     2761 gtgccgcgag ctggcggggg ttgaacgtat ctatgcaatg cgcaaaaaag ccgttggtct
     2821 gcttggcaat gccaaaggtg ccgctaagcc aattccgttt gctgaggata cctgcgtacc
     2881 gccggaacat ctggccgatt atattgctga atttcgcgcg ctgctcgaca gccacggctt
     2941 aagctacggt atgttcggtc acgtcgacgc aggtgttttg cacgtccgtc cggcactgga
     3001 tatgtgcgat ccgcaacaag agattttgat gaagcaaatc tctgatgacg tggtggcgct
     3061 gactgcgaaa tacggtggtt tgttgtgggg cgagcacggc aaaggttttc gcgctgaata
     3121 cagcccggcg tttttcggtg aggaactttt tgcagaactg cgcaaagtga aagcggcatt
     3181 tgacccgcat aaccgactca acccagggaa gatttgcccg ccagaaggtc tcgatgcgcc
     3241 gatgatgaaa gtggacgcgg tgaagcgcgg tacattcgat cggcagatcc ccattgcggt
     3301 acgccagcag tggcgcggtg cgatggagtg taacggcaac ggtttatgct tcaactttga
     3361 tgcccgtagt ccgatgtgtc cgtcgatgaa gatcacccag aaccggattc attcaccgaa
     3421 agggcgcgca acgctggtgc gtgaatggct gcgtttgttg gcggatcgcg gcgttgatcc
     3481 actcaaactg gaacaagaac tgcctgaatc cggcgtcagt ttacggacgt taattgcccg
     3541 cacgcgcaat agctggcatg cgaataaagg cgaatatgac ttctcacacg aagtcaaaga
     3601 ggcgatgtcg ggctgtctgg cctgtaaagc gtgttcgacc cagtgcccca tcaaaattga
     3661 tgtgccggag tttcgctctc gtttcctgca gctctatcac acccgttatt tacgcccgct
     3721 gcgcgaccac ctcgtcgcta cggtcgagag ctacgcaccg ctgatggcac gcgcgccgaa
     3781 aacctttaac ttcttcatta accagccgct ggtgcgcaaa ctctcggaaa aacatatcgg
     3841 catggttgat ttgccactgc tgtcggtccc ctcgctacaa caacaaatgg tggggcatcg
     3901 ctcggcaaac atgacgctgg aacagcttga agctctcaat gcagagcaga aagcgcgcac
     3961 ggtgttggtg gtgcaggacc cctttaccag ctattacgat gcgcaagtgg tggcggattt
     4021 tgtccgtctg gttgaaaaat taggtttcca gcctgtgtta ctgccatttt cgccaaatgg
     4081 caaagcccag catatcaaag gctttcttaa tcgttttgcg aagacggcga aaaagacggc
     4141 ggatttcctc aaccgtatgg ctaagcttgg gatgccaatg gtgggcgtcg atccggcgct
     4201 ggtactttgt tatcgcgatg aatataaact ggcgctgggc gaggaacgtg gcgagtttaa
     4261 cgtcttactt gcgaatgaat ggctggcaag cgcacttgag tcacatccag tggctacagt
     4321 cagcggtgaa tcatggtatt tctttggtca ctgtaccgag gttaccgctt tgccgggcgc
     4381 gccagcacaa tgggccgcga tatttgcccg ttttggcgcg aaactggaaa atgtcagcgt
     4441 gggttgctgc ggcatggcag ggacttacgg acatgaagcg aaaaaccatg aaaattcgct
     4501 cgggatctat gagttatcct ggcatcaggc tatgcagcga ctgccgcgta accgctgtct
     4561 ggcgaccgga tattcctgcc gtagccaggt aaaacgggtt gaaggcacgg gggtacgcca
     4621 tcctgtgcag gctttactgg agattattaa atgatatgga aacggaaaat caccctggaa
     4681 gcactgaatg ctatgggtga aggaaacatg gtggggttcc tggatattcg ctttgaacat
     4741 attggtgatg acacccttga agcgacaatg ccagtagact cgcggacaaa gcagcctttc
     4801 gggttgctgc atggaggagc atccgtggta ctggccgaaa gtatcggttc cgttgccggt
     4861 tatttatgta ccgaaggtga gcaaaaagtg gttggtctgg aaatcaatgc taatcacgtc
     4921 cgctcggcac gagaagggcg ggtgcgcggc atatgtaaac cgttgcatct cggttcgcgt
     4981 caccaggtct ggcagattga aatcttcgat gagaaagggc gtttgtgctg ttcgtcacga
     5041 ttgacgaccg ccattttgtg acgttgtcac cagaaaagtg tgacgcatat tgcttttggt
     5101 cattatctgc acagtaaacg gtggtatact gtgcacgtct tgtctaaaag cgaggatgct
     5161 atgtctactc aacttgatcc cacccagctg gccattgaat ttttacgccg tgaccagagc
     5221 aatctttcac ctgcccaata tctcaaacgt ctgaaacaac tagaacttga atttgctgac
     5281 ctgcttactc tctcttctac tgaattaaaa gaagaaatct actttgcctg gcgtctgggc
     5341 gtgcattgat cctgacttaa tgccgcaagt tcgccaaatc gacctggatt aacctccgtc
     5401 caggttcgat gaccttctta atactgctta tctaccgcct gatattgttc aaaaataata
     5461 ctaatcgcag tttgtgttaa aacggcgggt tagctttatg agattattct tatcgcccct
     5521 tcaagagcta agccactgag agtgccggag ataagcgccg gatggggtag aaacccttaa
     5581 gcctgtgtcg cacagactta agggttttct tatttctgaa cgctgcactt ttttacctat
     5641 ctcttaaata gtcattttca atacaacttt ttctttttca tcacagcctt aatgctcctt
     5701 agataataac cattatctaa caatgagata cctaattctt agcgtgcctg ttaacccaca
     5761 catcagggtc tatgcttatt aaaagcaggc aaaaagcatg ttttacggta aagcccctgc
     5821 gtttgctggg ttgaactgat aatcattatc actagcatgc tgttatacgc tgaaagcgat
     5881 gaagtgaggt aaatcgatgg acatgcattc aggaaccttt aacccacaag atttcgcctg
     5941 gcaaggctta acgctgacac ccgcagcggc gatacacatc cgtgagctgg tggcaaagca
     6001 gccgggtatg gtcggcgtgc gcttaggcgt gaagcaaacg ggctgcgcgg gctttggcta
     6061 tgtgctcgac agtgttagcg agccggacaa agacgatctg ctgtttgaac acgacggcgc
     6121 gaagctgttt gtcccgctgc aagcgatgcc gtttattgat ggcacggaag tcgatttcgt
     6181 tcgtgaagga cttaatcaga tattcaaatt tcacaaccct aaagcccaga atgaatgtgg
     6241 ctgtggcgaa agctttgggg tataggcggt actatgtctc gtaatactga agcaactgac
     6301 gatgtcaaaa cctggaccgg cggcccgctg aattataaag aaggattctt cacccagtta
     6361 gccaccgatg agctggcaaa ggggataaac gaagaggtgg tgcgcgcaat ttcggcgaag
     6421 cgtaatgagc cggagtggat gctggagttt cgtctaaacg cctatcgcgc atggctggag
     6481 atggaagaac cgcactggtt gaaagcgcac tacgacaagc tgaattatca ggattacagt
     6541 tactactcag caccatcgtg cggtaattgt gacgacactt gcgcgtctga acctggcgcg
     6601 gtgcagcaaa ctggcgcgaa cgccttttta agtaaagagg tggaggcggc gtttgagcag
     6661 ttgggcgttc ccgtgcggga aggcaaagag gtggcggtgg atgccatttt cgactctgtt
     6721 tcggttgcca ctacttatcg cgaaaaactg gcggagcagg gaattatttt ctgttccttt
     6781 ggtgaggcga tccacgatca tccggaactg gtgcgtaaat atctcggcac tgtggtgccg
     6841 ggaaatgaca acttctttgc cgcgcttaat gcggcggtag cctctgacgg tacgtttatt
     6901 tatgtgccta aaggcgtgcg ctgcccgatg gaactttcca cctattttcg cattaacgcg
     6961 gaaaaaaccg ggcagtttga gcgcaccatt ctggtggccg acgaagacag ctacgtcagc
     7021 tacattgaag gctgttccgc tccggtgcgt gacagctatc agttacacgc ggcggtggtg
     7081 gaagtcatca tccataaaaa cgccgaggtg aaatattcca cggtacaaaa ctggttccct
     7141 ggcgataaca acaccggcgg tattctcaat ttcgtcacca agcgtgcttt gtgcgaaggc
     7201 gaaaacagca aaatgtcatg gacgcaatca gaaaccgggt cagcgattac gtggaaatat
     7261 cccagctgca ttttgcgcgg cgataactcc attggtgagt tttactcagt ggcgctgacc
     7321 agcggtcatc agcaagcgga taccggcacc aagatgatcc acatcggtaa aaacaccaaa
     7381 tcgaccatta tctcgaaagg gatctctgcc ggacatagtc agaacagtta tcgcggctta
     7441 gtgaaaatca tgccgacggc aaccaatgcg cgcaatttca ctcagtgcga ctcaatgctg
     7501 attggcgcta attgtggggc gcataccttc ccgtatgtcg aatgtcgcaa taacagcgca
     7561 caactggagc acgaggcaac gacatcacgt attggtgaag atcaactgtt ttactgcctg
     7621 caacgcggga ttagcgaaga agacgccatc tcgatgattg ttaacggttt ctgcaaagac
     7681 gtgttctcag aactgccgct ggaatttgcc gttgaagcac aaaaactcct cgccatcagt
     7741 cttgaacaca gcgtcggata aggaataaac atgttaagta ttaaagattt acacgtcagc
     7801 gtggaagata aagctatcct gcgtggatta agcctcgacg ttcgtcccgg cgaagttcac
     7861 gccattatgg ggccaaacgg ttcgggcaaa agtaccttat cggcaacgct tgccgggcga
     7921 gaagattatg aagtgacggg cggcacggtt gagttcaaag gcaaagattt gcttgcgctg
     7981 tcgccggaag atcgtgcagg cgaaggcatc tttatggcct tccagtatcc ggtggagatt
     8041 cccggcgtca gtaaccagtt tttcctgcaa acggcactta atgcggtgcg cagctatcgc
     8101 ggccaggaaa cgctcgaccg ctttgatttt caggatttga tggaagagaa aatcgctctc
     8161 ctgaagatgc cggaagattt attaacccgt tcggtaaacg ttggtttttc cggcggcgag
     8221 aaaaagcgca acgatatttt gcaaatggcg gtgctggaac cggagttatg cattcttgat
     8281 gaatcggact ccgggctgga tattgacgca ttaaaagtgg ttgccgatgg cgtgaactcg
     8341 ctgcgtgacg gcaagcgctc attcatcatt gttacgcact accaacgcat tctcgactac
     8401 atcaagcctg attacgtcca tgtgctatat cagggacgaa ttgtgaaatc cggcgatttc
     8461 acgttggtca aacaactgga ggagcagggt tatggctggc ttaccgaaca gcagtaatgc
     8521 gctgcaacag tggcatcact tgtttgaagc tgaagggaca aaacgctcac cgcaagcaca
     8581 gcagcattta caacaattgc tgcgtaccgg actgccgaca cgtaaacatg aaaactggaa
     8641 atatacgccg ctggaagggc tgaccaatag tcagtttgtc agcattgcgg gagatatatc
     8701 cccacagcag cgtgatgcct tagcgttaac gttagacgcc gtgcggctgg tgtttgtcga
     8761 tggacgttac gtgcccgcgc tgagcgatgc aattgaaggc agcggatatg aagtgagcat
     8821 taacgacgac cgtcaggggt tacccgacgc tattcaggcg gaagtgtttc tgcatttgac
     8881 ggaaagcctg gcacaaagcg tgacgcatat cgccgtgaag cgcggtcaac ggccggcaaa
     8941 gccattgctg ttaatgcata tcacccaggg cgtggcaggt gaagaggtga acactgccca
     9001 ttaccggcat catctggatc tggcggaagg tgccgaagca acggtgatcg aacattttgc
     9061 cagccttaat gatgctcgtc attttaccgg ggcacggttc actatcaatg tcgcagcgaa
     9121 cgcccacttg cagcatatca agctggcgtt tgaaaacccg ctcagtcacc actttgctca
     9181 taacgatttg ttgctggctg aggatgccac cgcatttagc catagtttcc tgctgggtgg
     9241 cgcagtgtta cgacacaaca ccagtacgca actcaatggc gaaaacagca cgctgcggat
     9301 caatagcctg gcgatgccgg tgaaaaacga ggtgtgtgat acccgtacct ggctggaaca
     9361 caataaaggt ttttgtaaca gccgacagtt gcacaaaact atcgtcagcg acaaaggccg
     9421 cgcggtattt aacggtttga tcaacgtcgc gcagcacgcc atcaaaacgg atggtcagat
     9481 gaccaacaat aatctgctga tgggcaaaat ggcggaagtg gatacaaaac cgcagctgga
     9541 aatctatgca gatgatgtga aatgcagcca cggcgcgacg gtggggcgta ttgatgatga
     9601 acagatgttc tatctgcgct cgcgcgggat caatcagcag gatgcccagc agatgatcat
     9661 ttacgccttt gctgccgaac tgacggaagc actgcgtgat gaggggctta aacagcaggt
     9721 gctggcccga atcggtcaac ggctgccagg aggtgcaaga tgactttttc cgtcgacaaa
     9781 gtgcgggccg actttccggt gctttctcgt gaggtgaacg gtttgccgct ggcttatctc
     9841 gacagcgccg ccagtgcgca gaaaccaagc caggtgattg acgccgaggc tgagttttat
     9901 cgtcacggct acgcggcggt gcatcgcggt attcatacct taagcgccca ggcgaccgag
     9961 aaaatggaga acgtgcgcaa gcgggcatcg ctgtttatta atgcccgttc ggcggaagag
    10021 ctggtgttcg tccgcggcac gacggaaggg atcaatctgg tcgccaatag ttggggcaac
    10081 agcaacgtgc gggcgggcga taacatcatc atcagtcaga tggagcacca cgctaacatt
    10141 gttccctggc agatgctttg cgcacgcgtt ggcgcagagc tgcgtgtgat ccctctcaac
    10201 cccgacggta cgctgcaact ggagacgctg cctacgctgt ttgatgagaa aactcgcctg
    10261 ctggcaatta ctcatgtctc caacgtgctg ggaacagaaa atccgctggc ggaaataatc
    10321 acgcttgcgc accagcatgg cgcaaaagtg ctggtggatg gcgctcaggc ggtgatgcat
    10381 catccggtgg atgttcaggc gctggattgc gacttttacg tgttctccgg gcataaactg
    10441 tatggcccca ccggaattgg cattctttat gtgaaagaag ccttgttgca ggagatgccg
    10501 ccgtgggaag ggggcggttc tatgatcgcc accgtcagcc tgagtgaagg cactacctgg
    10561 accaaagcac catggcggtt tgaagccggt acacccaata ccgggggcat tattggtctt
    10621 ggcgcggcgc tggagtatgt ttcggcgctg gggcttaata acatagccga gtatgaacag
    10681 aatctgatgc attatgcgct atcacagctg gaatctgtac cggatctcac tctctatggc
    10741 ccacaaaaca ggcttggcgt tattgctttt aatctcggta aacaccacgc ctatgatgtt
    10801 ggcagttttc tcgataatta cggcattgct gtgcgtaccg gacatcactg cgctatgcca
    10861 ttaatggcct attacaacgt ccctgcgatg tgtcgggcgt cgctggccat gtataacacc
    10921 catgaagaag tggatcgtct ggtgaccggc ctgcaacgta ttcaccgttt gctgggataa
    10981 cagggaggca ctatggcttt attgccggat aaagaaaaat tgctgcgtaa ttttttacgc
    11041 tgcgccaact gggaagagaa atatctctac attattgagc tgggccagcg tctgactgaa
    11101 ttacgcgacg aagaccgaag cccacaaaat agcattcagg gctgccagag tcaggtgtgg
    11161 attgtcatgc gccagaatgc gcacggaatt attgaattgc agggcgacag cgatgcggcg
    11221 attgtgaaag ggcttattgc ggtagtcttt attctctacg atcagatgac gccgcaggat
    11281 attgtcagtt tcgatgtgcg tccgtggttt gaaaaaatgg cactcaccca acatctcacc
    11341 ccatctcgtt cacaaggtct ggaagcgatg attcgcgcaa ttcgcgccaa agccgctgca
    11401 cttagctaaa ctaaagggac agctttcatc cggttggctc acattgccgg atggtcaatg
    11461 tttacactga cctgcacagg agtgttttgg tttcaggtga acataagcgc catccgacgt
    11521 ttcagcgtga gtctccggca aatacaggag gtttacaatg aaacgcgcgt ctttgcttac
    11581 actcacgctt atcggcgctt ttagcgccat ccaggctgcc tgggcggttg attatccgct
    11641 accaccaacc ggaagccgac tggttgggca aaatcaaacg tatacggtgc aagaagggga
    11701 taaaaacctt caggctattg cccgacgttt tgatactgcg gcaatgttga tccttgaagc
    11761 caataacact atcgtcccgg tgccaaaacc tggtacgacg ataactattc cttcacaact
    11821 gttattacct gatgcaccgc gtcaggggat tatcgttaac cttgcagagc tgcgccttta
    11881 ttattatccg ccgggagaaa atattgtgca ggtttatcca ataggtattg gattgcaggg
    11941 gctggaaacg ccggtgatgg aaacgcgtgt tgggcagaaa atccctaacc caacctggac
    12001 gcctacggca ggcattcgtc agcgttcgct ggagcgtggc attaaattac cgccagtcgt
    12061 tcctgccgga ccaaataacc cgctaggacg ttacgcactg cgcctcgcgc atggtaatgg
    12121 cgaatacctc attcatggca ccagtgcgcc ggacagcgtt ggtttgcgcg tcagttcagg
    12181 gtgtattcgc atgaatgcac cggatattaa agccttgttc tccagcgtgc ggacgggaac
    12241 gccggtgaaa gtgatcaacg aaccggtgaa atattccgtg gaacctaacg ggatgcgtta
    12301 tgttgaagta catcgaccac tatcagcaga agaacagcaa aacgttcaga caatgccata
    12361 cacactgcca gcaggcttta cgcaatttaa agacaataag gctgtagatc agaagttagt
    12421 cgataaagcg ttgtatcgtc gggcagggta tccggtttcg gtgagcagtg gagcaactcc
    12481 cgcagccagc aatgcgcctt cagtagagtc agcgcagaat ggtgaaccag agcaagggaa
    12541 tatgttacgc gcgacgcagt agtggtaaat agcagacaaa aaaatggcgc acaatgtgcg
    12601 ccatttttca cttcacaggt actattactt gcggtattta gtagccatgt tgtccagacg
    12661 ctggttagca cgagctgcgt catctttagc agcctgaacg tcggaacgca ttgcgttcac
    12721 gtcgttgctc agctggtcaa ctttagcgtt cagagtctga acgtcagaag acagctgatc
    12781 gattttagcg ttgctggagc aacctgccag cagagtagaa cccaggatta ccgcgcccag
    12841 taccagttta gtagctttca ttattaatac cctctagatt gagttaatct ccatgtagcg
    12901 ttacaagtat tacacaaagt tttttatgtt gagaatattt ttttgatggg aaggcactta
    12961 tttttgatcg ttcgctcaaa gaagcatcga acgctgggtt ttagataaaa aaactgagag
    13021 aaaacaggat gcttccatcg gattcatctt agataaagtg agattatata gtgaaatccg
    13081 atttgatttt ttattgcttc tggttatcga ttaaataaaa aagcgcccat cagggcgctt
    13141 cgatatacaa attaattcac aaaagcaata ttacaggacg tgaacagatg cggtgttagt
    13201 agtgccgctc ggtaccagtg caccagaaac cataactacg acgtcacctt tgtgtgccag
    13261 accgctctgc agagccagtt ctttacccag acggtagaaa tcatcagtag aagtgatctc
    13321 tttaaccagc tgcggcacaa cgcctttgct cagtaccaac tgatgagccg ttttttcgtt
    13381 ggtggtcagt gccagtatgg tggcatccgg gaagtattta cgtactgcgc gagcagattt
    13441 accgccctgg gtagcaacca cgatcagcgg agcatccagt ttttcagcag tttcaacggc
    13501 accacggcat accgcttcgg taatgcgcag tttacggttg tcattgttga actcgagacg
    13561 gctgttcatc acgcggtcgg tacgttcgca gatggtcgcc atgatagaaa ccgcttccag
    13621 cgggtattta ccttttgcgg attcaccaga cagcatcact gcgtcagtac cgtcgaggat
    13681 ggcgtttgca acgtcaccgg cttctgcgcg agtcgggcgt gggtttttga tcatggaatc
    13741 cagcatctgg gtcgcagtga taacgacttt acgtgcacgg atacattttt cgatcatcat
    13801 cttctgggcg aagataactt cttctaccgg aatttctaca cccaggtcgc cacgcgcaac
    13861 catgatgccg tcagaggctt cgaggatttc gtcgaagttg ttgaggcctt cctggttttc
    13921 gattttggag atgatgtgga tgttttcgcc gccgtgcgct ttcaggtgct cacggatttc
    13981 gataacgtca gaacgcttac gaataaagga agcagcaaca aagtctacgc cttgttcgca
    14041 accaaagatc aggtcctgtt tgtctttttc agccagtgct ggcagggcaa tggaaacgcc
    14101 aggcaggttc acacctttgt tttcgcccag gtcgccgttg ttcagcactt tacagataac
    14161 tttgttacct tcaatggcag taacttccat accgatcaga ccatcgtcaa ccagtacggt
    14221 gttgccaaca gacaggtcag tagtgaaacc ttcatacgtt accgcaacca tttcgctgtt
    14281 gccgataaca gatttatcag tggtgaaagt aaaggtctga cccgctttca gagaaacgtc
    14341 gttaccgcct tccagtttca tggtgcggat ttccggacct ttggtatcaa gcaggatagc
    14401 ggcggtttta ccagttttgc tcatcacgtt gcgcagattc tgaatgcgct gaccgtgttc
    14461 tgcatagtca ccatgagaga agttcagacg cataacgttc atgccagcgt ccagcatttt
    14521 agctaacatc tcttcagatt cggttttcgg tccgatggtg caaacaattt tggtcttttt
    14581 catgacagtc ttagtcttta agttgagaag gatgggagaa acttgctttc tgggcgcact
    14641 gccggaaagc gaatccggta ttacgaaagt ggtggtgcct tgctctaagg ataggtgaca
    14701 tcgaaaaagc gtgcagagga atgtgtgctt gtgtctcagc ccaacgtgag tgcctgtttt
    14761 tgagtcgttg agttctacag tccaaagtgc tgaaaccatt caagagtcaa ttggcgcgca
    14821 ttatacgctg aaattaatcc aaaaggaaaa caaaaacagc gtttcaaaaa atatacaact
    14881 tgttagacaa taaattgtta tcaaagattt aacgttccag ggaaaaggtt acgcaaaaac
    14941 ttcgctgttt gcgcaacgtc tggtgccgaa gttgtcagtg tgaggataag tgaaagttca
    15001 tccggtgtat aaatatatca tcgctcaatt gctacagtta aaataattta tcgcgttatt
    15061 tgtctgattt tgtttgattt ttgtttaatt ggttagattg atatatgctg atagttggca
    15121 tgataaatag gtataaagaa tgggaaatcg gacaaaagag gatgaactgt accgagaaat
    15181 gtgcagagtt gtcggtaaag ttgtgctgga aatgcgcgat ctggggcagg aaccaaagca
    15241 tattgttatc gcaggcgtat tacgtactgc attagccaat aaacgcattc agcgcagtga
    15301 gctggaaaag caggcgatgg aaaccgtcat taacgcactg gtaaaatgac cattcaggat
    15361 gaggcggtaa gcttcatcct gatattcttg cttcagttgc ggtccagtta atcattctaa
    15421 ataacaactg ctgaaattat acgaattgcg gtatgtattt taatctataa cgataatatt
    15481 atttttattt tctgcaatg
//
LOCUS       DAZPLI010000092        15409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZPLI010000092 DAZPLI010000000
VERSION     DAZPLI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.093"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..454
                     /locus_tag="ACHUU3_002752"
     CDS             <1..454
                     /locus_tag="ACHUU3_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR2082773.1"
                     /translation="GLCMDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRIS
                     LPTTISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPP
                     PFHEIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            670..759
                     /locus_tag="ACHUU3_002753"
                     /pseudo
     CDS             670..759
                     /locus_tag="ACHUU3_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(760..1818)
                     /gene="modC"
                     /locus_tag="ACHUU3_002754"
     CDS             complement(760..1818)
                     /gene="modC"
                     /locus_tag="ACHUU3_002754"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR2082774.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1821..2510)
                     /gene="modB"
                     /locus_tag="ACHUU3_002755"
     CDS             complement(1821..2510)
                     /gene="modB"
                     /locus_tag="ACHUU3_002755"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR2082775.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2510..3283)
                     /gene="modA"
                     /locus_tag="ACHUU3_002756"
     CDS             complement(2510..3283)
                     /gene="modA"
                     /locus_tag="ACHUU3_002756"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR2082776.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3449..3598)
                     /gene="acrZ"
                     /locus_tag="ACHUU3_002757"
     CDS             complement(3449..3598)
                     /gene="acrZ"
                     /locus_tag="ACHUU3_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR2082777.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3727..4515
                     /gene="modE"
                     /locus_tag="ACHUU3_002758"
     CDS             3727..4515
                     /gene="modE"
                     /locus_tag="ACHUU3_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR2082778.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4583..6055
                     /gene="modF"
                     /locus_tag="ACHUU3_002759"
     CDS             4583..6055
                     /gene="modF"
                     /locus_tag="ACHUU3_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR2082779.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6316..7332
                     /gene="galE"
                     /locus_tag="ACHUU3_002760"
     CDS             6316..7332
                     /gene="galE"
                     /locus_tag="ACHUU3_002760"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR2082780.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7342..8388
                     /gene="galT"
                     /locus_tag="ACHUU3_002761"
     CDS             7342..8388
                     /gene="galT"
                     /locus_tag="ACHUU3_002761"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR2082781.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8392..9540
                     /gene="galK"
                     /locus_tag="ACHUU3_002762"
     CDS             8392..9540
                     /gene="galK"
                     /locus_tag="ACHUU3_002762"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR2082782.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9534..10574
                     /gene="galM"
                     /locus_tag="ACHUU3_002763"
     CDS             9534..10574
                     /gene="galM"
                     /locus_tag="ACHUU3_002763"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR2082783.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10777..11529
                     /gene="gpmA"
                     /locus_tag="ACHUU3_002764"
     CDS             10777..11529
                     /gene="gpmA"
                     /locus_tag="ACHUU3_002764"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR2082784.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11688..12740)
                     /gene="aroG"
                     /locus_tag="ACHUU3_002765"
     CDS             complement(11688..12740)
                     /gene="aroG"
                     /locus_tag="ACHUU3_002765"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR2082785.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13056..13436
                     /locus_tag="ACHUU3_002766"
     CDS             13056..13436
                     /locus_tag="ACHUU3_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR2082786.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13550..14491
                     /gene="zitB"
                     /locus_tag="ACHUU3_002767"
     CDS             13550..14491
                     /gene="zitB"
                     /locus_tag="ACHUU3_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR2082787.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14488..15207)
                     /gene="pnuC"
                     /locus_tag="ACHUU3_002768"
     CDS             complement(14488..15207)
                     /gene="pnuC"
                     /locus_tag="ACHUU3_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR2082788.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15245..>15409)
                     /locus_tag="ACHUU3_002769"
     CDS             complement(15245..>15409)
                     /locus_tag="ACHUU3_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="3 different subfamilies; catalyzes the formation of
                     quinolinate from iminoaspartate and dihydroxyacetone
                     phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR2082789.1"
                     /translation="SCAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNR
                     MLDFAATLRG"
BASE COUNT         3916 a         4045 c         3851 g         3597 t
ORIGIN      
        1 cggcctttgc atggatcttg cactggtaac aggacagcgc gaaggagatc tggcggctat
       61 gagatgggag gatataagag atggcaggct atacgtggaa caacaaaaaa caggggcaaa
      121 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      181 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      241 gacaatcgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      301 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      361 cggtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      421 tgacggacga gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaaca
      481 ccacttgtaa ttaattgatt ataaatgatt aattcctaat gggcatttgc ttcatgaatg
      541 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      601 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      661 aaattcttaa atgacgcggt aaaagcgcgc gccaacattg tgattggtga taacaccacc
      721 gacagcattg cccagttcat ttatagccac ctgatttaat caggcggtta tcgacacact
      781 tttaatttgc gcgtacagcc acaggccagg tttgatcgcc agttcatccc tggcccacgg
      841 gctgatacgc gcccacagcg ttttaccgcc gacttccagc tccacttcca cctggccgtt
      901 gtcgtcataa ctattaacaa cttttgcccg caatacgtta cgaatgctgg tttgctgcgg
      961 cggttgtaaa accagagaaa catcggaagc ctgaatgcgg atacgtagcg cagcttgcag
     1021 cggttcgtcc agcttattga cccacaaatg ctgatcgccc agcgccagcg cggtcatcgc
     1081 gtaatgcgga tgatgctcaa gtaccgtcac tttcagaatg ctactttgtt gctctttcgg
     1141 cagccacgga ttcatcacgc tactgcccca cacttcctcc agcgcgccaa aggctttcac
     1201 ctgaccgttt tccagtacca tcactctgtc tgccagatgg aggatctcat ccagcgaatg
     1261 gctgacatac aacatcggaa tgttgatttc ccgtgtcagc cgttgcagat aaggcaacag
     1321 ttcgcgttta cgcggaatat ccagtgacgc cagcggttca tccagcaaca gcaattccgg
     1381 tgctgtcagc aaagcccgac caatcgccac gcgctgtttt tcgcctccgg acaggctgcc
     1441 tggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc accagcttat cgaactgatc
     1501 gaccatactt ttcgacatgc cgtagcgcag attgccacgc actttgtaat gcgggaacag
     1561 ccgcgcatcc tgaaaaacat agccaacgcg acgcttttcc ggcgtcaggc agataccttt
     1621 ttcggcatca tttagtaccc gcccattgag gacaatccgc cctttttgcg ggcgcgtcag
     1681 tccactgatg gcgttaatca gcgaagtttt tccggcaccg gagacgccaa agatagcagt
     1741 gatgccattg gcgggcagtg tttcattaat ggtcaggcaa tggttgccca acgtctggga
     1801 aaaattcagc tccagcatga ttagcgcccc gcccgttcac ggctgattct ggccagccat
     1861 tctgaaatca acagggagat catcgccagc gcaatagaaa taatgcacag tctcgccgcg
     1921 ccactttccc cgccgggggt ctggatcagg gtatacatgg cagagggaat ggttcgcgtt
     1981 tcgccaggaa tgttcgacac aaaggtgatg gttgcaccaa actcaccgag agaacgagca
     2041 aaagccagta ccgtaccaac aataattccc ggtaaggtca gcggtaacgt gatagtaaag
     2101 aaaacgcgcc agcgcccggc acctagtgtt cttgcggcct gttcgagttt gacatcaacc
     2161 ccttccagcg ccagacgaat tgcccgcacc atcagcggaa acgacatgac ggcggcagcg
     2221 agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa accagtcata cagacgttca
     2281 ccgataaatc cgcgccgtcc catcgaaact aataataagt aaccgacgac cacgggcggt
     2341 aacaccagcg gtagatgcag tacgctgtcg agcagagctt tgcccggaaa cgtgcaacgc
     2401 accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa acagcacagc cagggaagaa
     2461 actttcaggc ttaataaaac tgcctgccat tctggatcgg tcagtatcat tacttggttg
     2521 taaatccgta acgtttaaag atttccgctg cctgcggtcc cttcagataa tcataaaatg
     2581 ctttcactgt cgcattgtta tgcccttcca caacagcaac cggatattcc acttttttat
     2641 gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt gctgacaact gcgtcagaac
     2701 cgtaaacaat gcccaacggc gcttcattgc gttcgaccaa cgccagcgcg ccacgaacat
     2761 cttccgccgg agccagtttc ggggagagcg tatcccatgc gcccagtttt tgcagtgctt
     2821 cttttgcata aatgccagca gggacatgtt ccggatcgcc aaccgcgagg cgaccgccat
     2881 tcagcagcga agtccagtta gttgtgctgt cgatgatgaa atctttctgc tcgctggctt
     2941 tcggtgctac aacgaccagg ctattgccga gcagtgtctg gcgcgaagcc gtatcgatcg
     3001 cttttttatc aaccgcataa tccatccatt tctgatcggc agagataaac agatccgcag
     3061 gcgctcccgc ttcaatctga cgggccagag tagatgacga agcgaaagaa gaaaccacat
     3121 ccacgccttt ttctttttta tactgcgtag caatgtcctg cattgcgtta gtcagtgatg
     3181 cggcggcgaa caccgtgatt ttcccttcat ctgccagtgt attgccagca acagcgaaag
     3241 agagtgccgc cccggcaaac aagttcaacc atttacgagc cattggtaac cccttaatgt
     3301 aacgttatat aggtgacaat ataacggcta gctcagaatt ttcccagcag ttattgctaa
     3361 cctttgtgag gtagataaga aaaaatatcg gctggaaaag caggaagttg agaaaaagaa
     3421 atgcccgact aagcgggcat tcagggaatc aatgattttg tccgggctgg tcttttttac
     3481 caacaccaga aaagatgttg aatacttcac caagaccgta aatcagaccc aggatgatgg
     3541 ccatcacgac aggtaccatg attacggcga ataccagact ttttaataac tctaacatgg
     3601 tcaactccag atatagtcat gagactattc taaccgctaa gcacagaaaa gcactcccct
     3661 tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt ttgccaggac
     3721 attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt attcgccgac
     3781 ccgcgccgga tttcgctact aaaacacatt gcgctttccg gttccattag ccagggagcg
     3841 aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat gaatcagtta
     3901 agcgagcata ttctggttga gcgcgcaaca ggcggtaaag gtggcggcgg cgcagtactg
     3961 acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat ccagcaaaaa
     4021 gcctttgatg tgttaagtga cgatgacgcc ctgccgctga acagcctgct ggccgcgatc
     4081 tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat caccgcccgc
     4141 gatcatgatg acattcaaca gcatgttgat gtcttactgg ctgacggaaa aacacgcctg
     4201 aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg caaagaagtg
     4261 ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt cgcacaaaac
     4321 gccgacaacc aattaccggg tattattagt catattgagc gcggcgcaga gcagtgcgaa
     4381 gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt aaatgaagcg
     4441 acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag cgtgattatc
     4501 gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct gtcagtaatc
     4561 gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca cgtttcgtct
     4621 tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg atagttgggc
     4681 gtttgtcggt tcgaatggaa gcgggaaatc ggccctggcc cgcgcgctgg cgggggaact
     4741 tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc tctccttcga
     4801 gcaattgcaa aaactcgtca gcgacgaatg gcagcggaat aacaccgata tgctcggccc
     4861 tggcgaagat gacaccggac gcactacggc tgaaatcatt caggatgaag taaaggatgc
     4921 accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg accgacgctt
     4981 taaatacctt tccactggcg agacgcgaaa aaccctgctg tgtcaggcgc tgatgtcgga
     5041 gcctgacttg ttgattcttg atgagtcgtt cgatggcctg gatgttgcct cacgtcagca
     5101 gctggctgag cgactcgcct cgttacatca gtccggtatt actctggtac tggtgctcaa
     5161 tcgcttcgat gagatcccgg agtttgtcca gtttgctggc gtgctggcgg attgcacgtt
     5221 agcggaaact ggcgctaaag aggaactgct ccaacaagca ctcgtcgcgc aactggcgca
     5281 tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag cacgtcacgc
     5341 cttacccgcc aacgaaccgc gcattgtgct gaacaatggc gtggtttctt ataacgatcg
     5401 ccccattctt aataacctta gctggcaggt gaatccaggt gaacactggc aaattgtcgg
     5461 gccaaatggt gcaggaaaat cgacgttatt aagcctggtt actggcgatc atccgcaagg
     5521 ttacagcaac gatttgacgc ttttcggacg acgtcgcggc agcggcgaaa ccatctggga
     5581 tatcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc gggtcagcac
     5641 taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt atcaggccgt
     5701 ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca ttgataaacg
     5761 tacggctgac gctccgttcc atagtctttc ctggggacag cagcgtctgg cgctgatcgt
     5821 ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac aggggcttga
     5881 tccgctcaat cgccagctta tccgccgttt tgttgatgtg ctgattagcg aaggtgaaac
     5941 gcaattgtta tttgtttcgc accacgctga agatgcgcct gcctgtatta cccatcgcct
     6001 tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat attgagtcgg
     6061 tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt tcgacgcatg
     6121 caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa accctctgtt
     6181 ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg attccactaa
     6241 tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca taccataagc
     6301 ctaatggagc gaattatgag agttctggtt accggtggta gcggttacat tggaagtcat
     6361 acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa cctctgtaac
     6421 agtaagcgca gcgtactgcc tgttatcgag cgcttaggcg gcaaacatcc aacgtttgtt
     6481 gaaggcgata ttcgtaacga agcgttgatg accgagatac tgcacgatca cgctatcgac
     6541 accgtgatcc acttcgccgg gctgaaagcc gtcggcgaat cggtacaaaa accgctggaa
     6601 tattacgaca acaatgtcaa cggcactctg cgcctgatta gcgccatgcg cgccgctaac
     6661 gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc caaaattcca
     6721 tacgttgaaa gcttcccgac cggcacaccg caaagccctt atggcaaaag caaattgatg
     6781 gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg actggagcat tgccctgctg
     6841 cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga tccgcaaggc
     6901 attccgaata acctgatgcc atacatcgcc caggttgctg taggccgtcg cgactcgctg
     6961 gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga ttacatccac
     7021 gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa caagccaggc
     7081 gtacacatct acaacctcgg tgctggcgta ggcagcagcg tgctggacgt ggttaatgcc
     7141 ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg cgaaggcgac
     7201 cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg gcgcgtaacg
     7261 cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca tccacaggga
     7321 tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc acatcgccgc
     7381 tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa gcgcccctgg
     7441 cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc agattgcttc
     7501 ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc ccgattacac cgggacttac
     7561 gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc agaaagcaac
     7621 gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg cttttcaccg
     7681 gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat cgtcaaaacc
     7741 tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt ctttgaaaac
     7801 aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg ggcaaatagc
     7861 ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt tgccgaacag
     7921 aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag ccgtaccgtt
     7981 gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc gttcgaaacg
     8041 ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc ccagcgcagc
     8101 gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt ccagtgctcc
     8161 ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa tcaacactgg
     8221 cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg taaatttatg
     8281 gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca ggcagcagag
     8341 cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga aatgagtctg
     8401 aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac tcacaccatt
     8461 caggcgcctg gccgcgtgaa tttgattggt gaacacaccg actacaacga cggtttcgtt
     8521 ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga tgaccgtaaa
     8581 gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct caatgcgccc
     8641 attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt gaaacatctg
     8701 caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa tgtgccgcag
     8761 ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt attgcagcag
     8821 ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga agcagaaaac
     8881 cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact cggcaagaaa
     8941 gatcatgcct tgctgattga ctgccgctca ctggggacta aagcagtttc catgccgaaa
     9001 ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg cagcgaatac
     9061 aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc agctctgcgc
     9121 gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat cgtggcgaaa
     9181 cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag cgcgctggag
     9241 caaggcgatc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc tatgcgcgat
     9301 gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa agcagtgatt
     9361 ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat cgtcgcgctg
     9421 atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata tgaagcaaaa
     9481 acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg acagtgctga
     9541 acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact ttgcgtaaca
     9601 acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttca gctcgtattc
     9661 cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagcccg gaatgctatc
     9721 aggatcaggc cgcgtttctg ggggcctcta ttggtcgtta tgccaaccgc atcgccaata
     9781 gccgttatac ctttgacggt gaaacggtga cgctttcgcc aagtcagggc gttaaccagc
     9841 tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac cagaacgatc
     9901 gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg ggtaatctcg
     9961 gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact tatcgcgcca
    10021 cagttgataa atcttgcccg gtgaatatga ctaatcacgt ctatttcaat cttgacggcg
    10081 agcagtctga cgtgcgcaat cacaagttgc agattctggc gggcgaatat ctgccggttg
    10141 atgaaggcgg cattccgcac gacggcctga aatctgtcgc cggaacctct tttgatttcc
    10201 gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc aaagtgaaag
    10261 gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg gcggcgcatg
    10321 tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccc gctcttcaat
    10381 tctactccgg caacttcctc ggcggcacac cgtcgcgggg aaccgaacct tacgccgact
    10441 ggcaaggact ggcgttggaa agcgagtttc tgccggacag cccgaaccac cctgaatggc
    10501 cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg gaatatcagt
    10561 ttattgctga gtaatgttaa gccctcgtca tgagggcttt atcttcatat tgttcaaatc
    10621 accagcaaac accgacatat ttgcaactca atattcacaa caaccttaca ctgcgccact
    10681 attttcgcta tggttatgcg taagcattgc tgttgcttcg tcacggcaat ataatgagaa
    10741 ttattatcat aaaaagataa tttgaggagt aagtatatgg ctgtaactaa gctggttctg
    10801 gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt tcaccggttg gtacgacgtg
    10861 gatctgtctg agaaaggcgt aagcgaagca aaagcagcag gtaagctgct gaaagaggaa
    10921 ggttacagct ttgactttgc ttacacttct gtgctgaaac gcgctatcca taccctgtgg
    10981 aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg agaaatcctg gaaactgaac
    11041 gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg aaaccgccga aaagtatggc
    11101 gacgagcagg tgaaacaatg gcgtcgtggt tttgccgtga ctccgccgga actgactaaa
    11161 gatgatgagc gttatccggg tcacgatccg cgttacgcga aactgagcga gaaagagctg
    11221 ccgctgactg aaagcctggc gctgaccatt gaccgcgtga tcccttactg gaatgaaact
    11281 attctaccgc gtatgaagag cggtgagcgc gtgatcatcg ctgcacacgg taactcttta
    11341 cgtgcgctgg tgaaatatct tgataacatg agtgaagaag agattcttga gctgaatatc
    11401 ccgaccggcg tgccgctggt gtatgagttc gacgagaatt tcaaaccgct gaaacgctat
    11461 tatctgggta atgctgacga gatcgcagcg aaagcagcgg cggttgctaa ccagggtaaa
    11521 gcgaagtaaa cgtcattcgt ttaaaaatga gaaagccgac tgcaagtgag tcggcttttt
    11581 tgttgctaac aatggagcac attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg
    11641 tgattcatcg gatacgccac tctgacggcg catccgacaa ttaaacctta cccgcgacgc
    11701 gcttttactg cattcgccag ttgacgtaac agagcatcgg tatcttccca gccaatgcag
    11761 gcatcggtga tgctcttacc gtaggccagc ggctccccgc tatcgaggct ctgattgcct
    11821 tccaccagat ggctttccac catcacgcca ataatggcct tttcgccacc ggcaatctgc
    11881 tggcaaacgt cagcacaaac atccatctgc tttttgaatt gtttggacga gttagcatgg
    11941 ctgaaatcga tcatcacctg tgctggcagg cctgctttgt tcagcccttc tttcacttca
    12001 gcaacgtgct tcgcgctgta gttaggctct ttaccgccgc gcagaatgat atggcaatca
    12061 ccgttaccgc tggtattcac aatcgccgaa tgcccccatt tagttacgga caggaagcag
    12121 tgcggcgcac cggcggcatt aatggcatcg atagccactt taatcgtacc gtctgtgcca
    12181 tttttaaaac ctaccggaca agaaagacca gacgccagtt cgcggtgcac ctgcgattcc
    12241 gtggtacgtg caccaattgc gccccagctc atcaggtcag cgagatactg aggagtgatc
    12301 atatcgagaa actcacccgc cgctggcaga ccgctgtcgt taatatcaag cagcaattta
    12361 cgggctatac gcagaccgtc gttgatctgg aagctgttat ccatatgcgg atcgttaatc
    12421 aaccctttcc agcccaccgt ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc
    12481 agctcatctt tcagctcttc acgcagcgcc agcaagcgag tggcatactc tttagccgcg
    12541 acaggatcat gaattgagca cgggccaatc accaccaaca ggcgatcatc attacctttc
    12601 aggatcttat ggatcgcttt tcgggcatgg gcaaccgtat tcgcggcatt ttcagttacg
    12661 gggaattttt ccagcaatgc gacaggagga agtaactctt tgatttcttt gatgcgtaaa
    12721 tcgtcgttct gataattcat gtctgttcca gtgttgccat acttatctta gtgaatgcaa
    12781 tacttccaat ctatatcttc cgtcagaatg tgtaaacggg gttttacact atgaacggat
    12841 taatcctgga attagcaaga aaaacgccag attgtcgcga aaaacgagat ctctcctaca
    12901 atttctaact gtaactcctt tgtttgttaa ttatttcaag attctctgct gcgtttcata
    12961 aactggctga aatcttaaac caatgcctta tattcacctg caaatgcact gttggaagag
    13021 gttatccgac ataacgacca taacaggagc atcctatgaa aatgacaaaa ctggccacat
    13081 tatttctgac tgccactcta agccttgcca gcggtgccgc actggctgcc gatagcggag
    13141 cgcaaactaa taacggccag gcaaacgccg cagctgatgc gggccaggta gcccctgacg
    13201 cccgtgaaaa tgtcgcgcca aataacgtcg acaataacgg ggtaaatacc ggttctggcg
    13261 gcacaatgct gcattcggat ggttcttcaa tgaacaatga cggaatgacc aaagatgaag
    13321 agcacaagaa caccatgtgc aaagatggtc gatgtccgga tatcaataaa aaggtacaaa
    13381 tcggtgatgg catcaataat gatgtcgata ccaaaaccga cggtaccacg cagtaaccct
    13441 gcaaaatagc caataatggg agagcggagt ctctcccttt taatttttca cttctaaaag
    13501 cagtatgatg tcataaagtt aagtgagaat aattaaggga tgactcgtca tggcgcactc
    13561 acactcacac acgtcctcac acctgccaga agataataat gctcgtcgct tgttgtatgc
    13621 tttcggcgtg actgccgggt ttatgctggt agaagtcgtt ggtggttttc tttctggttc
    13681 tctggcattg ctggccgatg cgggtcatat gttgaccgat actgccgccc tgcttttcgc
    13741 cctactcgcc gtgcaatttt cacgtcgtcc tcccaccatt cgccacactt tcggttggct
    13801 aagactgacc acgctcgcgg cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt
    13861 gattgtctgg gaggcgatag aacgcttccg cacgccgcgt ccggtcgagg gcggcatgat
    13921 gatggcaatt gccgtggctg ggttgctggc aaatatactt tctttctggt tacttcatca
    13981 cggcagcgaa gagaaaaacc tcaacgtacg agcagcggca ttgcatgtgc tcggggacct
    14041 gttgggttcg gttggggcta ttattgccgc gttgattatt atctggaccg gctggacacc
    14101 tgctgacccc attctctcga tagtggtgtc gcttctggtt ctgcgtagcg cgtggcgact
    14161 gttgaaagat agcgtgaatg aattacttga aggtgcaccg gtatcgctgg atatcgctga
    14221 actgaagcgt cgtatgtgcc gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt
    14281 atggatggta ggcgagaagc cggtgataac gctgcatgtg caggtgatcc cgccacacga
    14341 tcacgatgcc ttgttggatc agatccaaca ctacctgatg gatcactatc agattgagca
    14401 cgccaccatt cagatggaat atcaaccttg tcatgggcca gactgccacc tgaatgaagg
    14461 ggtgtctggc cattcacatc atcaccatta atgggacagc gcgcgtgagc ctctttcacg
    14521 tgcgctgttg atccacatcc gactgccgtt gagcgcaata aaggtcagga tgatgtactc
    14581 cagagacatg gcgtaaacgc cctgaagtgc aaaaataacg acgctaatca cgttaataat
    14641 cacccacaac agccagtttt ccacatactt acgcgtcatc agaatcattg ccacgataga
    14701 taacaccatc atgcatgaat cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac
    14761 aacctgtaat cctaatgctt gcatgatcat gactgccacg cgggtcaaaa atgcaaacac
    14821 cggattgata aataccgtca tcagaccaat cgaaacaacg caaaccgcca accagctgag
    14881 cgccttcggc aatggtaacc agcgaatttt caactccgcc tcgttctgac tggtttgtcg
    14941 cgaccacgca taccaaccgt aaatattcgc ggcaaagaaa aacacctgta atagcaggct
    15001 ggcatacagc tgaatctgaa agaaaataat gccaaacaag gtgacgttga tcaggccaaa
    15061 gaagtagttg ctgatcttct ccagactggc aaggccaata cacagcaacc cggcgatcgt
    15121 gcctaccgct tcgatccatg agagatcata accgcctgcc cctattggta tatgtaccag
    15181 gatattctgc acactaaaaa aatccatctt tcccccaaag cgtaacgtta cgccttatta
    15241 ttcgttatcc acgtagtgta gccgcaaaat ccagcatacg attgagcggc accagcgccc
    15301 tctctcgcag cctttcatca agaacctcgt gattgcttcc ttcctgttct aatgcctctg
    15361 cgatggactg aaggccattc atggccatcc acggacaatg cgcgcagct
//
LOCUS       DAZPLI010000093        15386 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZPLI010000093 DAZPLI010000000
VERSION     DAZPLI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.094"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            107..2641
                     /gene="mrcB"
                     /locus_tag="ACHUU3_002770"
     CDS             107..2641
                     /gene="mrcB"
                     /locus_tag="ACHUU3_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR2082790.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            2861..5050
                     /gene="fhuA"
                     /locus_tag="ACHUU3_002771"
     CDS             2861..5050
                     /gene="fhuA"
                     /locus_tag="ACHUU3_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR2082791.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            5101..5898
                     /gene="fhuC"
                     /locus_tag="ACHUU3_002772"
     CDS             5101..5898
                     /gene="fhuC"
                     /locus_tag="ACHUU3_002772"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR2082792.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5898..6788
                     /gene="fhuD"
                     /locus_tag="ACHUU3_002773"
     CDS             5898..6788
                     /gene="fhuD"
                     /locus_tag="ACHUU3_002773"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR2082793.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6785..8767
                     /gene="fhuB"
                     /locus_tag="ACHUU3_002774"
     CDS             6785..8767
                     /gene="fhuB"
                     /locus_tag="ACHUU3_002774"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR2082794.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8925..10205)
                     /gene="hemL"
                     /locus_tag="ACHUU3_002775"
     CDS             complement(8925..10205)
                     /gene="hemL"
                     /locus_tag="ACHUU3_002775"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR2082795.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            10371..10418
                     /gene="yadX"
                     /locus_tag="ACHUU3_002776"
     CDS             10371..10418
                     /gene="yadX"
                     /locus_tag="ACHUU3_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR2082796.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            10430..11851
                     /gene="clcA"
                     /locus_tag="ACHUU3_002777"
     CDS             10430..11851
                     /gene="clcA"
                     /locus_tag="ACHUU3_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR2082797.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            11875..11940
                     /gene="yadW"
                     /locus_tag="ACHUU3_002778"
     CDS             11875..11940
                     /gene="yadW"
                     /locus_tag="ACHUU3_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR2082798.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            11933..12277
                     /gene="erpA"
                     /locus_tag="ACHUU3_002779"
     CDS             11933..12277
                     /gene="erpA"
                     /locus_tag="ACHUU3_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR2082799.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(12324..12947)
                     /locus_tag="ACHUU3_002780"
     CDS             complement(12324..12947)
                     /locus_tag="ACHUU3_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR2082800.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(12985..13785)
                     /gene="btuF"
                     /locus_tag="ACHUU3_002781"
     CDS             complement(12985..13785)
                     /gene="btuF"
                     /locus_tag="ACHUU3_002781"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR2082801.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(13778..14476)
                     /gene="mtnN"
                     /locus_tag="ACHUU3_002782"
     CDS             complement(13778..14476)
                     /gene="mtnN"
                     /locus_tag="ACHUU3_002782"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR2082802.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            14560..>15386
                     /gene="dgt"
                     /locus_tag="ACHUU3_002783"
     CDS             14560..>15386
                     /gene="dgt"
                     /locus_tag="ACHUU3_002783"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR2082803.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVA"
BASE COUNT         3478 a         3866 c         4299 g         3743 t
ORIGIN      
        1 ggagagggga ccgatcgagc acaaattttg agagatatct tcttctgtct tgtaacagaa
       61 gaacagaaaa tcgggctttt gcgcctgaat attgcggaga aaaagcatgg ccgggaatga
      121 ccgcgagcca attggacgca aagggaaacc gacgcgtccg gtcaaacaaa aggtaagccg
      181 tcgtcgttac gaagatgacg atgattacga cgattatgat gactatgagg atgaagaacc
      241 gatgccgcgc aaaggtaagg gcaaaggcaa agggcgtaag cctcgtggca aacgcggctg
      301 gctatggctg ctgttaaaac tggctatcgt ttttgccgtg ctgatcgcca tttacggcgt
      361 ttatctcgat caaaaaattc gtagccgtat tgatggcaag gtctggcaac tgcccgcggc
      421 agtttatggc cgaatggtca accttgaacc agatatgacc atcagcaaga acgaaatggt
      481 gaagctgctg gaggcaacac agtatcgtca ggtgtcgaaa atgacccgtc ctggcgaatt
      541 taccgtgcag gccaacagca ttgagatgat ccgccgtccg tttgatttcc cggacagcaa
      601 agaagggcag gtgcgtgcgc gtctgacctt tgatggcgat catctggcga cgatcgtcaa
      661 tatggagaac aaccgtcagt tcggtttctt ccgtcttgat ccgcgcctga tcaccatgat
      721 ctcttcgcca aacggtgagc agcgtctgtt tgtgccgcgc agtggtttcc cggacttgct
      781 ggtggatact ttgctggcga cagaagaccg tcatttctac gagcatgatg ggatcagtct
      841 ctactccatc ggacgtgcgg tgctggcaaa cctgaccgcc ggacgcacgg tgcagggagc
      901 gagtacgctg acgcaacagc tggtgaaaaa cctgttcctc tccagcgagc gttcttactg
      961 gcgtaaagcg aacgaagctt acatggcgct gatcatggac gcgcgttaca gcaaagaccg
     1021 tattcttgag ctgtatatga acgaggtgta tctcggtcag agcggcgaca acgaaatccg
     1081 cggcttcccg cttgcgagct tgtattactt tggtcgcccg gtagaagagc tcagcctcga
     1141 ccagcaggcg ctgttagtcg gtatggtgaa aggggcgtcc atctacaacc cgtggcgtaa
     1201 cccgaaactg gcgctggagc gacgtaatct ggtgctgcgt ttgctacaac aacagcagat
     1261 tatcgatggt gaactgtatg aaatgctcag cgcgcgtcct ctgggcgtac agccgcgcgg
     1321 tggggtaatc tctccacagc cagcctttat gcaactggtg cgtcaggagc tgcaggcgaa
     1381 attaggcgat aaggtaaaag atctctccgg cgtgaagatc ttcactacct ttgactcggt
     1441 ggcccaggac gcggcagaaa aagccgccgt ggaaggcatt ccggcactga agaaacagcg
     1501 taagttgagc gatcttgaaa ctgcaattgt ggtcgtcgac cgctttagtg gtgaagttcg
     1561 tgcgatggtg ggaggttctg agccgcagtt tgcgggctac aaccgtgcga tgcaggcgcg
     1621 tcgttcgatt ggttcccttg caaaaccagc gacttatctg acggctttaa gccagccgaa
     1681 aatctatcgc ctgaacacgt ggattgccga tgcgccaatc gcgctgcgtc agccgaatgg
     1741 ccaggtctgg tcaccgcaga acgatgaccg tcgttatagc gaaagcggca gagtgatgct
     1801 ggtggatgcg ttgacccgtt cgatgaacgt gccgacggta aatctgggga tggcgctggg
     1861 gctgcctgca gttacggaga cctggatcaa actgggcgtg ccgaaagatc agctgcatcc
     1921 ggttccggcg atgctgctgg gggcgttgaa cttaacgcca atcgaagtgg cgcaggcatt
     1981 ccagaccatc gccagcggcg gtaaccgtgc accgctttct gcactgcgtt cggtaatcgc
     2041 ggaagatggc aaggtgctgt atcagagctt cccgcaggca gaacgcgctg ttccggcgca
     2101 ggcggcgtat ctgacactat ggaccatgca gcaggtggta caacgtggta cgggtcgtca
     2161 acttggggcg aaatacccga acctgcatct ggcagggaaa acagggacta ccaacaataa
     2221 cgtagatacc tggtttgcgg gcattgacgg cagcacggtg accatcacct gggtaggccg
     2281 tgataacaac cagccgacca aactgtatgg tgccagcggg gcaatgtcta tttatcagcg
     2341 ttatctggct aaccagacgc caacgccgct gaatcttgtt ccgccagaag atattgcaga
     2401 tatgggcgtg gactacgacg gcaactttgt ttgcagcggt ggcatgcgtg tcttgccggt
     2461 ctggaccagc gatccgcaat cgctgtgcca gcagagcgag atgcagcagc agccgtcagg
     2521 caatccgttt gatcagtctt ctcagccgca gcaacagccg caacagcaac ctgctcagca
     2581 agagcagaaa gacagcgacg gtgtagccgg ttggatcaag gatatgtttg gtagtaatta
     2641 acatctaagc gtgaaatacc ggatggcgaa ttgccatccg gtaaaataac atcccaccta
     2701 aagtattaac cctttctttt catctggttg tttattaacc cttcaggaac gctcagattg
     2761 cgtaccgctt gcgaacccgc cagcgtttcg aatattatct tatctttata ataatcattc
     2821 tcgtttacgt tatcattcac tttacatcag agatatacca atggcgcgtt ccaaaactgc
     2881 tcagccaaaa cactcactgc gtaaaatcgc agttgtagta gccacagcgg ttagcggcat
     2941 gtctgtttat gcacaggcag cggttgaacc gaaagaagac actatcaccg ttaccgctgc
     3001 acctgcgccg caagaaagcg catgggggcc tgctgcaact attgcggcga agcactctgc
     3061 tactgcgact aaaacggata caccaattga aaaaacgccg cagtctattt cggttgtgac
     3121 aaatgaagag atgcagatgc gtcaatttca gtctgtaaaa gaagcattag gttatacacc
     3181 tggcgttacg gttagcagtc gtggtgcttc taatacatat gattttgtta tcattcgtgg
     3241 tttctcatct gttggtctga atcaaaataa ttaccttgat ggactaaaac ttcagggcaa
     3301 cttttataac gatgctgtga ttgatcctta catgcttgag agggttgaac tgatgcgtgg
     3361 gccgacgtct gttctctatg gaaaaagtaa tcctggtggg attatttcta tggtgagtaa
     3421 gcgtccgacc actgaacccc tgaaagaaat tcagtttaaa atggggacgg ataatctgtt
     3481 tcagaccgga ttcgatttca gtgatgcact ggatgataac ggtgagttct cttaccgttt
     3541 gacgggcctc gctcgctcaa caaacgaaca gcagaaaaac tctgaatctc agcgttatac
     3601 cattgctcca tcattctcat ggcgtccaga cgacaaaacc aattttactt tcctgtccta
     3661 tttccagaat gaaccagaaa cgggttatta cggttggttg ccgaaagagg ggaccgttga
     3721 gccattgcct aatggtaagc gtctaccgac tgacttcaat gaaggtgcat cgaataatac
     3781 atactcccgt aaccagaaaa tggtgggata tagttttgaa catggtttta atgacacctt
     3841 caccgtgcgt cagaatctgc gtttcagtga aatgaaaacc tcacagaaaa gtgtttatgg
     3901 cacagggatt gctggtgatg gtcataccct aaaccgcggg acagtggtgg ataatgagcg
     3961 tctacaaaac tttagcgttg atacccaact tgaaagtaag tttgctacag gtgacgtcga
     4021 gcatactttg ctgacagggg tagacttcat gcggatgcgt aatgatatca atgccagctt
     4081 tggatccgca ccatccatcg atctttataa caaatatcac cctgaatact ttgcatttgg
     4141 tagcgcagag ccataccaga tgaatgaaag caaacaaaca ggtatttatg ttcaggatca
     4201 ggcggaatgg aataaatggg tattcactct ggggggacgc tacgattggt ctaagcaagc
     4261 gaccactgtt cgtgaaaact cttatacgcc gactgaaggt tatattgagc gcaatgatca
     4321 tcagttcacc tggcgcggtg gtgtaaatta cttattcgat aatggtattt caccttactt
     4381 tagctatagc cagtcctttg aaccgagtgc tttcgatctg tggagcaacc cgcgcatttc
     4441 ctataagcca tcgaaaggtg aacagtatga agctggcgta aaatatgttc cgaatgatat
     4501 gccggtcgtt gttacgggcg cagtctatca attgacgaaa acaaataacc tgacagcaga
     4561 cccaacaaac ccattagcgc aagtcccagc aggtgagatt cgcgctcgtg gtgtggaact
     4621 tgaagcaaaa gcggcgttaa atgccaatat taacttgacg gcttcttata cctacaccga
     4681 tgcggaatac accaaagaca ccaatctcaa aggtaaaact ccagaacaag taccggagca
     4741 tatggcatct ctctgggggg attatacctt caatgaaggg ccgctttctg gtttaacatt
     4801 gggaacaggt ggtcgtttta ttggttccag ctatggtgat ccggcaaaca cttttaaagt
     4861 gggtagcgca gctgtaatgg atgctgttgt aaaatatgat ctggcacgct ttggtatggc
     4921 gggatccagc cttgctgtga acgtcaacaa tttgctcgat cgtgagtatg ttgccagttg
     4981 cttccagact tatggctgct tctggggcgc agaacgtcag gtcgttgcaa ccgcaacctt
     5041 ccgtttctaa tttctctttt ggggcacgga tttccgtgcc catttcacaa gttggctgtt
     5101 atgcaggaat acacgaatca ttccgatacc acttttgcac tgcgtaatat ctcctttcgt
     5161 gtgcccgggc gcacgctttt gcatccgctg tcgttaacct ttcctgccgg gaaagtgacc
     5221 ggtctgattg gtcacaacgg ttctggtaaa tccactctgc tcaaaatgct tggtcgtcat
     5281 cagcctccgt cagaagggga gattcttctt gatgcccagc cgctggaaag ctggagcagc
     5341 aaagcgtttg cccgcaaagt ggcttatttg ccgcagcagc ttcctccggc agaagggatg
     5401 accgtgcgtg aactggtggc gattggtcgt tacccgtggc atggcgcgct ggggcgcttt
     5461 ggtgcggcag atcgcgaaaa ggtcgaggaa gctatctcgc tggttggctt aaaaccgctg
     5521 gcgcatcggc tggtcgatag tctctctggc ggcgaacgtc agcgggcgtg gatcgccatg
     5581 ctggtggcgc aggatagccg ttgtctgttg ctcgacgaac cgacttcggc gctggatatc
     5641 gcccaccagg ttgatgtgct ggcgctggtg caccgtttaa gtcaggaacg tggcctgacg
     5701 gtcattgccg tgttgcacga tatcaatatg gcggcacgct actgtgatta tctggtcgcc
     5761 ctgcgcagtg gtgaaatgat tgctcaggga acgcctgcgg aaattatgcg cggcgaaacc
     5821 ctcgaaatga tttatggcat cccgatgggt attttgccgc atccggcggg tgctgcacct
     5881 gtgagttttg tttattgatg agcggcttac ctcttatttc gcgccgtcga ctgttaacgg
     5941 cgatggcgct ttctccgttg ttatggcaga tgaataccgc ccacgcggcg gctattgacc
     6001 ccaatcgtat tgtggcgctg gagtggttgc cggtggaatt actgctggcg ctcggcatcg
     6061 tgccttacgg cgtggcggat accatcaact atcgcctgtg ggtcagcgaa ccaccattgc
     6121 cggactcagt gattgacgtc ggtttgcgta cagaacctaa ccttgaactg ctgaccgaaa
     6181 tgaaaccgtc gtttatggtc tggtcggcag gatatggccc ttcatcagaa atgctggcac
     6241 gtattgcgcc gggtcgcgga tttaacttca gtgacggcaa acagccgttg gcgatggcgc
     6301 gtaaatcgct gacggaaatg gcagatttac ttaacctgca aagcgcagcg gaaacgcatt
     6361 tagcgcaata cgaagacttt atccgcagca tgaaaccccg ctttgtgaag cgtggcgcac
     6421 gcccgttact gctgacgacg cttatcgatc cgcgccatat gctggtcttc ggtccaaaca
     6481 gcttgttcca ggaaattctc gatgagtacg gcatcccaaa tgcctggcaa ggggaaacca
     6541 acttctgggg cagtaccgcc gtcagtatcg atcgtctggc agcgtataaa gacgttgatg
     6601 tgctctgttt tgatcacgac aacagcaaag acatggatgc gctaatggca accccgctgt
     6661 ggcaggccat gccgtttgtc cgcgccggac gctttcagcg cgtacctgca gtctggtttt
     6721 atggtgcgac actctcggca atgcactttg tgcgcgttct ggataacgcc atcggaggta
     6781 aagcgtgagt aaacgaattg cgctttttcc ggcgttattg ctggcgctgt tagtgattgt
     6841 cgctacggcg ctcacctgga tgaacttctc gcaggcgctg ccgcgtagcc agtgggcgca
     6901 ggctgcctgg tcgccagaca tcgatgtcat cgagcagatg atttttcact acagcttgtt
     6961 gccgcgtctg gcgatttcgc tgctggtggg cgcgggcctg gggctggtgg gcgtgctgtt
     7021 tcagcaagtg ctgcgtaacc cgctggcgga gccgacgacg cttggcgttg ctacaggcgc
     7081 gcaactggga attaccgtca ccacactttg ggcgatccca ggtgctatgg cgagccagtt
     7141 tgccgcactg gtgggcgctt gtgtcgttgg cttaatcgtc tttggcgtcg cgtgggggaa
     7201 acggctttcg ccggttacgc ttattctcgc ggggctggtg gtgagccttt attgcggggc
     7261 aattaatcag ctactggtta tcttccatca cgaccagctg caaagcatgt tcctgtggag
     7321 caccggaacg ctgacgcaaa ccgactgggg cggcgttgag cgtttatggc cgcagttgct
     7381 gggcggcgtg atgttgacgt tattgctact tcgcccgtta accctgatgg ggcttgatga
     7441 tggcgtggcg cgcaatctcg ggctggcctt gtcgctcgcg cgtctggcgg cgctgtcgct
     7501 ggcgatagtc atcagtgcgc tgctggtgaa tgcggtgggg attatcggct ttatcggttt
     7561 gttcgcgccg ctgctggcga aaatgctggg ggcgcggcgt ttgttgccac ggttgatgct
     7621 ggcgtcgctg attggggcgc tgatcctctg gctttccgat caaatcatcc tctggctgac
     7681 tcgcgtgtgg atggaagtgt ccaccggttc ggtcactgcg ttgatcggtg cgccgctgct
     7741 actgtggctg ttgccgcgtt tacgcagcat tagcgcgccg gatatgaagg tcaacgatcg
     7801 tgtcgcggct gaacgccaac atgtgctggc gtttgccctc gcgggcggcg tgctgctgtt
     7861 gatggctgtg gtggtggcgc tgtcgtttgg tcgtgatgcg cacggctgga cgtgggcgag
     7921 cggggcgttg ctcgaggatt tgatgccctg gcgctggccg cgaattatgg cggcgctgtt
     7981 tgcgggcgtc atgctggcgg tggcgggctg tattattcag cggctgaccg gaaacccgat
     8041 ggcaagcccg gaagtgctgg ggattagctc tggcgcggcg tttggcgtgg tgttaatgct
     8101 gtttctggtg ccgggtaatg cctttggctg gctgttaccc gcgggcagta tcggggcggc
     8161 ggtgacgctg ttgatcatta tgatcgccgc cggccgcggt ggattttccc cacaccgtat
     8221 gttactggcg gggatggcgt taagtaccgc attcaccatg cttttgatga tgttgcaggc
     8281 gagcggtgac ccgcgaatgg cgcaagtgct gacctggatt tccggttcga cctacaacgc
     8341 gaccgatgcg caggtctggc gtaccggaat tgtgatggtg attttgctgg cgattacccc
     8401 gctgtaccgc cgctggctga ccattttacc gctgggtggt gataccgcac gagccgtagg
     8461 aatggcgctg acgccgacgc gaattgcgct gctgctgtta gcggcttgcc tgacggcgac
     8521 cgcgacgatg actattggac cgttgagttt tgttggttta atggcaccgc atattgcgcg
     8581 gatgatgggc tttcgacgga cgatgccaca catcgtaatt tcggcgctgg tgggtggttt
     8641 actgctggta ttcgctgact ggtgtgggcg gatggtgctg tttccattcc agatcccggc
     8701 ggggctgctg tcgaccttta tcggcgcgcc atattttatc tatttgctga gaaagcagag
     8761 ccgttaattt ttccatataa cggccctgta cgcctggacg gataaagcgt ttacgcgaca
     8821 acggatgccc gatgcgacgc tggcgcgtct tatcgggcct acaaacggcc ccgaatcgta
     8881 ggtcggataa ggcgttcacg ccgcatccga caaaccatgc tggatcacaa cttcgcaaac
     8941 acccgacgtg cagcatcgat ggtgttattg atatcttcca tgctgtgcgc cacggacata
     9001 aagcccgctt caaacgctga cggtgccagg taaacacctt cgtccagcat catatggaag
     9061 aaacgcttaa agcgttccac gtcacaggcc atcacatcct gatagcacgt cacggactcg
     9121 gcgtcggtaa agaaaatacc gaacatgccg ccaacgtggt taacgaccag cggaattccg
     9181 gcttcttctg ccgcttccag cagaccttct gccagacgtg ttgtcagctc atccaacgtt
     9241 tcgtgaacgc ccggttgcgc gacttcattc agacaggcga aaccggctgc catcgcaatt
     9301 gggttaccgg aaagcgtacc cgcctgatag accggacccg tcggggccag cgcatccatt
     9361 acatcacgac gaccaccgaa tgcgcctacc ggcattccac cgccgatgat tttgcccagg
     9421 caggtgagat ccggttccac accgtaataa tcctgtgcgc cagccagtgc cacgcggaag
     9481 ccggtcatta cttcatcgat gatcagcaat gcgccaaatt cgtcgcacag cgcacgcaga
     9541 cctggcagga agtctggcag cggtggaaca cagttcatat tgcctgccac cggctcgatg
     9601 ataatacagg caatctcttg cgggtattgc tcaaacgcgg cgcgtacaga agccagatcg
     9661 ttataagtac aggttaaggt atgtttggcg aaatctgccg gaacgcccgg cgagtttggc
     9721 tggcctaacg tgagtgcgcc agaaccggct ttcaccagca ggcagtcagc gtgaccgtgg
     9781 taacaccctt caaatttaat gattttgtcg cgaccggtaa aaccacgggc cagacggatg
     9841 gcgctcatcg tcgcctcggt gccggagttc accatgcgca ccatatccat ggtcgggacc
     9901 aattcggtca ccagttgcgc cattttcact tccatttcgg ttggtgcacc aaagcttaaa
     9961 ccacgctcgg cggcttcaat cacggcattg cggattgccg gatggttatg gcccagcacc
    10021 atcggccccc aggaaccgac ataatcgata taggctttgc catcaacatc gtacagataa
    10081 gcgccgtccg ctttttcgat aaacagtgga gtgccgccca cgccagtaaa ggcgcgaaca
    10141 ggggagttca caccgccagg gatcagctcg cgcgctgcgc tgtaaagatt ttcagactta
    10201 ctcatggagg gttcctgatt cgtagaaaaa gtgaatggct gctattctat gttattcata
    10261 acaagttaaa tactcgtcaa acatcaggct gcttgtactg gtgcaatcct gatttcttag
    10321 agtataaaag ttttgtgcat ttgaaacatt acgctttgca aaggattttc atggaacgtg
    10381 cgagtaaaat gccgtcatct tatttgtatg accaataagt gatcattgga tgaaaactga
    10441 tactccctct ttagaaacac cgcaggccgc gcgcctgcga cgcagacaac tgattcgcca
    10501 acttcttgag cgcgataaaa ccccgttagc cattttgttt atggcggcag tcgtcggcac
    10561 gcttgttggg ctggcagcgg ttgcttttga caaaggtgtc gcctggttgc agaaccaacg
    10621 tatgggggcg ctggtacata ctgctgataa ttatccgctt ctgttaaccg tcgcttttct
    10681 ctgttcggcg gtgctggcga tgtttggcta ctttttggtg cgcaaatacg cgccggaagc
    10741 aggtggttcg gggatcccgg aaattgaagg ggcgctggaa gatcaacgtc ccgttcgctg
    10801 gtggcgtgta ttgccggtga agttctttgg cgggctgggg acactcggcg gaggcatggt
    10861 gttggggcgc gaagggccaa ccgtgcagat cggcggtaac attggccgta tggtgcttga
    10921 tattttccgc ctgaaaggtg acgaagctcg ccatacgctg ctggcaaccg gtgctgctgc
    10981 ggggctggct gcggccttta acgcgccgct ggcgggtatt ttgtttatta tcgaagagat
    11041 gcgtccgcag tttcgctata cgttaatttc gataaaagcg gtatttattg gcgtcattat
    11101 gtcgaccatt atgtaccgga tttttaatca tgaagttgcg ttgattgacg tcggtaaact
    11161 ttctgatgcg ccgcttaata cgctgtggct ttatctgatc ctcggtatta tttttggcat
    11221 tttcggccct atttttaata aatgggtgct ggggatgcag gatttgctgc accgtgtgca
    11281 cggcggcaat attaccaaat gggtactgat gggcggtgcg attggcggtc tgtgtggatt
    11341 gctggggttt gtggcaccag caacgtcggg cggcggtttt aacctgattc ctatcgctac
    11401 cgcggggaat ttcagcatgg gaatgctggt gtttatcttc gtcgctcggg tcattaccac
    11461 cttactctgc ttctcttccg gcgcgccggg cggtattttt gccccgatgc tggcgctggg
    11521 tactgtgctg ggaaccgctt tcggaatggt tgccgttgag ctgtttccgc aatatcacct
    11581 tgaggcgggg acgtttgcta ttgccggaat gggggcatta ctggcggcat ctattcgcgc
    11641 gccgttaacg gggatcattc tggttctgga gatgaccgat aactaccagc tcattttgcc
    11701 aatgattatt accggtcttg gcgcaacact attagcgcaa tttaccggcg ggaaaccgct
    11761 atactcggcg attcttgcgc gcacgctggc aaaacaggaa gctgagcaac tggcgcgaag
    11821 caaggccgca tcagccagcg agaatacttg aacgaaatac cagggtatta gataatggcg
    11881 attattattg ggttagaatt tgcccaattg ccgatgtcgt ttggagcaaa atatgagtga
    11941 tgacgtagca ctgccgctgg agtttaccga cgcagcagcc aacaaagtta aaagcctgat
    12001 cgctgacgaa gataacccga atctgaaatt acgcgtgtat atcacaggtg gcggttgcag
    12061 cggcttccag tatggtttca cctttgatga tcaggtgaac gaaggcgata tgaccatcga
    12121 aaaacagggc gttggcttgg tggttgatcc gatgagcctg caatatctgg tcggcggttc
    12181 cgttgactat accgaaggac tggaaggttc tcgtttcatc gtgaccaacc cgaacgcgaa
    12241 aagcacctgc ggttgcggtt cttcctttag catctaatct gttgttcatg tgccggataa
    12301 actatccggc gcatcttctc tcttcaacgc ccattctcat ccagcgcaaa cgtcggtagc
    12361 ttaagatgcc aacgaatagc cgccagccga atcaatagcg tcacgaccat gcccatcata
    12421 ctggctgttt ccagtggtac ggaaaatgtg taataagccg tagcgtggac aataccgccg
    12481 ataatacagg cagttgcgta gatttctgta cgtaaaatca tggggatctc gcgggccagt
    12541 acatcacgaa tgatcccgcc gccaacgcca gtaatgacga ccatacaaac cgcgattaac
    12601 ggaccgactt ccgcattaaa ggctttattc acgccaatgc cgacaaacac cgccagacca
    12661 acggcgtcca gcaccggcaa catccatttt ggtaagcgtc ttggctggcg caccagcacg
    12721 atggtcagca tgctggttac cattgcaacg accagatcgg tgggatcttt cacccaaaat
    12781 accgggccgt gatccagcgc catgtcgcga attgtcccgc cgcctacagc ggtaaccacg
    12841 cccagtacca gaacaccaaa agggtccata cgcaattttc cggctaacaa aacgccggag
    12901 atggcaaata ccgctgtgcc gactatatcc agccaataga cgagcattgt tcaatcccca
    12961 ctgagcacct aaaaaggtca ggcgctaatc tacctgtgaa agcgcattac agagctgttg
    13021 tgcagcgagg ataatacgtg ggcttgcacg ttcaaaccag tcactcgtga gaggaataac
    13081 gggaattttg agctgttcac cccagtattg tttgatttta ggaatttggt ccggtccgcc
    13141 tgtaatgaca atcgcctgtg gcgagcgtgc taacacctgt tcgcggctaa cttgcggcca
    13201 gggaacccgg ctgtctttaa agatgttttc tccgccacaa acttcgagta cctggttttg
    13261 aatcgactct tttccactgg taaatggcgg attaatgccg aattgcagaa aaacacgttt
    13321 tttaggttta tcagcatatt gcgctttcaa ttgcgcgtac tgatccagca gggattgcgc
    13381 ggcttgttcg gccttatccg gttgcggact ccagggggcc aattgacgta acgcattggc
    13441 aatttgttca atgcttgtcg catcgaccca catcactttt attcccagcg aagccagctg
    13501 gtcaacctgc cgctcggcat tacctccacg ccaggcaatc accagatcgg gtttcagcgc
    13561 gacaatgcgt tccagattca tcccctgcca ggtggaaacc tgctcaatct tttgcgcttg
    13621 tggaggatag tcggaatagc tgctgacccc aaccggcgtg atcccggcgg caaaggcaag
    13681 ttcagtgttg gcgggagaaa gcgtgatgac gcgcggcgcg gcgttgagcc acagtggcgc
    13741 aagaaaagac agggcgacca gcgccctgaa cagtgactta gccatgtgcc agtttctgca
    13801 ccagtgactc aaccatcagg ctggactgtt tagcggcaac agccaggaac tcatcgaagc
    13861 taagatgaga ctgttgatcg gccacgtcgg agatggcgcg tacgacaaca aacgggacgt
    13921 tgaaattgtg gcagacatgg gcgattgccg tcgcttccat ctctacagca atggcctgtg
    13981 ggaagttgtg gcggattttc gccagaccaa cagaaccgtt gatgaaagcg tcgccgctaa
    14041 caatcaggcc acgtacagcg ttaagattca gttcggcaat gcaggcctca gcggcagcga
    14101 tcagtttatc gtcagcttta aagcctgccg gacagcctgg taactgaccg tattcataac
    14161 caaatgccgt gacatccgcg tcgtgataac gcgcttcgtc cgagacaacg atatcgccca
    14221 ctttcagcgt tggtgccagg ccaccggcag aaccggtgtt aataatcaca tctggcttgc
    14281 agtgttccaa cagcagagtg gcacccagcg ccgcagcgac tttaccgatg cccgatttca
    14341 gaagcgcaac ctcggttcca ttcagttggc cggtatagat ttcgcaaccg ccgagactga
    14401 tagtttgacg gttttcgatt ttgtcacgca gcagcgtaac ttcttcttcc attgcaccaa
    14461 tgatgccgat tttcatagat ttactcgcga taagcccgat ttgaaagcat agtttaccat
    14521 gcgcttacgg ggaagcgtat ttctcacgcg ggagaggaca tggcacagat tgatttccga
    14581 aaaaaaataa actggcatcg tcgttaccgt tcaccgcagg gcgttaaaac cgaacatgag
    14641 atcctgcgga tcttcgagag cgatcgcggg cgtatcatca actctccggc aattcgtcgt
    14701 ctgcaacaaa agacccaggt ttttccactg gagcgcaatg ccgccgtgcg cacgcgtctt
    14761 acccactcga tggaagtcca gcaggtggga cgctacatcg ccaaagaaat tttaagccgt
    14821 ctgaaagagc ttaaattgct ggaagcatac ggcctggatg aactgaccgg cccctttgaa
    14881 agcattgttg agatgtcatg cctgatgcac gatatcggca atccgccgtt tggtcatttt
    14941 ggcgaagcag cgataaatga ctggtttcgc caacgtttgc acccggaaga tgccgaaagc
    15001 cagcctctga ctgacgatcg ctgcagcgtg gcggcactac gtttacggga cggggaagaa
    15061 cagcttaacg agctgcggcg caagattcgt caggacttat gtcattttga ggggaatgca
    15121 caaggcattc gtctggtgca tacattgatg cggatgaatc tcacttgggc acaggttggc
    15181 ggtattttaa aatatacccg tccggcgtgg tggcgtggcg aaacgcctga gacacatcac
    15241 tatttaatga aaaagccggg ttattatctt tctgaagaag cctatattgc ccggttgcgt
    15301 aaagaactta atttggcgct ttacagtcgt tttccattaa cgtggattat ggaagctgcc
    15361 gacgacatct cctattgtgt ggcaga
//
LOCUS       DAZPLI010000094        15342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZPLI010000094 DAZPLI010000000
VERSION     DAZPLI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15342
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.095"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..1015
                     /locus_tag="ACHUU3_002784"
     CDS             <1..1015
                     /locus_tag="ACHUU3_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR2082804.1"
                     /translation="KISSDGCLKKEQMVVSAIASTPQLPMLGEKRLVSSVTKEDLLFV
                     RRDLLTGYQKLSNGKISSIKGRSVVTVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPL
                     KKSKIEPDPLTRDEFIRFIEACRHQQTKNLWIIAVYTGIRHGELVSLAWEDIDLKART
                     ITIRRNYTKLGEFTPPKTDAGTGRTIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREY
                     GRSTVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYA
                     CWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNKRLSESVPCMPHK
                     KVG"
     gene            1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHUU3_002785"
     CDS             1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHUU3_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFR2082805.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1797..1904
                     /locus_tag="ACHUU3_002786"
     CDS             1797..1904
                     /locus_tag="ACHUU3_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR2082806.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHUU3_002787"
     CDS             complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHUU3_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR2082807.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHUU3_002788"
     CDS             complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHUU3_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR2082808.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="ACHUU3_002789"
     CDS             complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="ACHUU3_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFR2082809.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="ACHUU3_002790"
     CDS             complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="ACHUU3_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR2082810.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="ACHUU3_002791"
     CDS             complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="ACHUU3_002791"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR2082811.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8366..10081
                     /gene="dld"
                     /locus_tag="ACHUU3_002792"
     CDS             8366..10081
                     /gene="dld"
                     /locus_tag="ACHUU3_002792"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR2082812.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="ACHUU3_002793"
     CDS             complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="ACHUU3_002793"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR2082813.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11226..11813)
                     /locus_tag="ACHUU3_002794"
     CDS             complement(11226..11813)
                     /locus_tag="ACHUU3_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR2082814.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11983..12561
                     /locus_tag="ACHUU3_002795"
     CDS             11983..12561
                     /locus_tag="ACHUU3_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR2082815.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12691..13452)
                     /locus_tag="ACHUU3_002796"
     CDS             complement(12691..13452)
                     /locus_tag="ACHUU3_002796"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR2082816.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="ACHUU3_002797"
     CDS             complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="ACHUU3_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFR2082817.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15165..15248
                     /gene="yohP"
                     /locus_tag="ACHUU3_002798"
     CDS             15165..15248
                     /gene="yohP"
                     /locus_tag="ACHUU3_002798"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFR2082818.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3744 a         4178 c         3838 g         3582 t
ORIGIN      
        1 aaaaatatca tcagatggct gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag
       61 tacacctcag ttgccaatgt taggtgaaaa aagactggtt tcatctgtaa caaaagagga
      121 tttgcttttc gtaaggagag atctgttgac cggttatcaa aagctttcta acggaaaaat
      181 ttcttccata aaaggacgct cagtggtcac agtaaactac tatatgacaa ccatagctgg
      241 aatgtttcaa tttgcaacag ataatggtta tacatcagga aatccattta acggtctgac
      301 accattaaaa aagtccaaga tagaaccaga tcccctcacc cgtgacgaat ttattcgttt
      361 tattgaggct tgccgtcatc aacaaacaaa aaacctgtgg attatcgctg tatacacggg
      421 tattcgtcac ggggagctgg tatcactggc ctgggaagat atagacctta aagcgaggac
      481 tataaccatc cgccggaatt atacaaaact aggcgaattc actccaccaa aaactgatgc
      541 tggtaccgga agaacgattc atctagttca accagctatt gatgctctta aaagccaagc
      601 ggaaatgacc atgcttggaa aacagcattc tgtagaggta aagcagaggg agtatgggag
      661 aagtactgtg cataaatgca cttttgtttt tagtcctcag gtaataaaac agcggcagtt
      721 ttccggaccg cactataagg ttgactccat cagggagtca tggacaagta tcttaaaacg
      781 cgcaggtctg agacacagaa aatcgtacca atccaggcat acttatgcat gctggtcact
      841 tgccgctgga gctaatccta gttttatcgc aagccagatg ggccacacaa acgcacaaat
      901 ggtattcaat gtttacggag catggatgaa agacaacaat cacgaacaga tagaactcct
      961 taacaaaaga ctatctgaaa gtgtcccatg tatgccccat aagaaagtgg ggtaaaataa
     1021 aaacttgtaa aatcagttag tttaccctta atccctgtca cgttacgcgc gtggcagagg
     1081 cgttacgggt tgctgaaacc gcaacggaca gacggcggtc atcgactgtt caacgatgcc
     1141 gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa
     1201 gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa
     1261 gaaacattac tgacctacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa
     1321 gagcgcggtc aggattaccc cgcccagaca ctcaccacac atctgtttat tcctctgcgc
     1381 cgacggcttc agtgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta
     1441 ctgatcaact acatcgccat ttgtctggct tcggcacgta aaaaacaggg taaagatgcg
     1501 ctggtggttg gctggaatat tcaggatacc acccgtctgt ggctggaggg ctggattgcc
     1561 agtcaacaag gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa
     1621 ctattcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag
     1681 cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg catttaatga
     1741 ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag tgcaatatgc
     1801 gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc ggaattggtg
     1861 gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct
     1921 ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc cctggatcac
     1981 atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc
     2041 taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc
     2101 cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacgactcat
     2161 tcccatacct ttcgcaactt ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt
     2221 cgcctgcagg acgggcagca caccgtaaag gatcaaggcg ataatcgctg gttgcagacc
     2281 aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag tctgtccaac
     2341 ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag tgacagcaat
     2401 tccggcacca gtgccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg
     2461 agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca gttgtgggaa
     2521 caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagtgcca caaacaaagc
     2581 aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc tccagcagat
     2641 cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc
     2701 atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct
     2761 caccttctgc ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcaggc
     2821 gcacacccag ttcgctgcgc ccaaaaaact ggcggacaaa atcgtttgcc ggacgagtca
     2881 gcatcgtcag cggattgccc tgctgcacca cttcaccgtg atccatcaat accagatgtt
     2941 ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatggta cgccccagca
     3001 aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagtgcgc
     3061 caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca
     3121 cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgtaaa tttgactcca
     3181 gccccagtag cgccattaat tcgtcgatac gatcgtcaat cctcgcccgc gaccattttt
     3241 gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgggggaac aggccaatag
     3301 attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag cgaatttctt
     3361 ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc attttcaggg
     3421 tggtggattt gccggagcca gatgtgccaa tcagcaccga aaaactccct tcctgaaaat
     3481 tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca tggccaaatt
     3541 caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc gtcggtaagc
     3601 accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc gctgcttagc
     3661 agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc
     3721 atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgccagt
     3781 ggtaactgaa catgcaggaa tcgctgcgcc ccgctcatgc ccatcgctct ggcgctctcc
     3841 agcacatcgc gcgggatctg gttcaaaccg actaccacgc cgcgcaccag cggcagcaag
     3901 gcatagagca ccagcgcaat cagtgcgggt gtcattccgg ttcctgctat gccgagcgtc
     3961 cccagccacg gaaaggccgt aaccagcgcg gcaagcggcg caatcaacag gccaaagagc
     4021 gccaccgaag gcacggtctg aatgacattg agcagagaaa aaattgcccc ctgccgagca
     4081 gtggaaaagt agcaccagat gcccaacggc acaccaatca ctaacgcagg cagcaccgca
     4141 ccaaacagca acgtcagatg ttgtgccagc gcgtcgtcaa acacatcctg acggttggcg
     4201 tattctttca ttagtgagag atcgttaagc gtgccggagt acagcaacca cagcggaata
     4261 atggcaatct gcatatgcaa caaccagcgc cacagcggat gcgtggagat tcggcggatg
     4321 gcatcactac aggccagcaa tgccagcgcc gcggccagcc agaaaccact gccgaggctg
     4381 gtacgcgcca gcgcactgcc attttgcgcc agttgggtcg ccgcctttcc tgctccccac
     4441 accagcaata cgaagacgaa ttgcgccaga atgagtgcaa aaatgctgcc ttttttagcg
     4501 ggaataaaac aggctgtcag ccaggcgcaa ccaacgccca ccagcatcca gatcgtttgc
     4561 ggccacagtt gccagagatg acgcccttca cccgagacta aacgattagg cgcgtaactg
     4621 ataaacggca gcgccgctgc gattgccgtc agcaacagca gcagcgccag aacaggatta
     4681 atacgtagat aagtcacggg aaattacttc gtccaccctt tttgtttcag gtagtcggca
     4741 gccacttttt tggcatccag tccttccact gcaatgctgg cattcagttg ctgcaatgtt
     4801 tttgcatcga ggctggcgaa gactggctgt agccactgtg ccatttgcgg atactctttc
     4861 agcaccgact cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc tttcggatcg
     4921 cttaaggttt gcagccccag cgccgcgacc ggaccgtcag tgccgtaagc cattgcggca
     4981 ttaacgccag aagtttgctg ggcagcggct ttaatcgtta ccgccgtgtc gccgccagcc
     5041 agtgacagta actgatcctg atcgagttta aagtcgtagg ctttttcaaa cgcgggtaac
     5101 gcatcggcgc gttcgataaa ctctgccgag gctgccagtt tgaaggtgcc gccctctttc
     5161 agataacgac tcaggtcggc aagcgaagtg agtttgtttt tctctgccac atcctgacgc
     5221 acggcgatgg tccaggtgtt attcgctggt gcgggcgtca gccagattaa cttgttttgc
     5281 tctgcatcga gttttttgac tttctcgtaa ccttgctgcg cgtttttcca tgccgcatcg
     5341 ttttcatctt taaagaagaa agcgccatta ccggtatatt ccggatagat atccagttca
     5401 ccggaagtaa tcgccccgcg caccacagga gtcgttccga gttgcacttt attcaccgtc
     5461 ggtacgccgt ggctttccag cacctgcaag atgatgttgc cgagtagcgc gccttcggta
     5521 tcgatttttg aaccgacttt aacgggggaa gccgcctgta gcggcaggct cacggctgcc
     5581 aacataacca gtgaacctgc ccagaccttt gagagtggca tgatgctttc ctcattcttt
     5641 tactgttgtt ttcagcgaat taagagaaaa gcatagttga taatgacaga gttagcctga
     5701 gcggaaggat tcagttgcag aatcagataa atacttaaag ggcatatttt gcccggcgtt
     5761 tcttgccgga tgcggcgtaa acgccttatc cggcctacaa aggtcgaaaa ttcagcaagt
     5821 taagatatga acatcaggca aagggcgcaa acgtcgcgcc cttactaaag cattacagca
     5881 actcaaactc gcctttctta acgcgtgcgg aatcagtgcc gataaagaca ttgaacttgc
     5941 caggttcggc gtcatatttc atctgttgat tccagaactt cagcgcctcg atatcaatcg
     6001 ggaagctgac agtctgagtt tcgcccggtt tcagggtgat tttctcaaag cctttcagct
     6061 gcttcactgg acggctcatg gaagccgtca catcctgcaa gtacatctgt actaccgtgg
     6121 ccccttcgcg tttaccggtg ttcgtcacct gcacgctggc ggtcactttg ccgtcacgct
     6181 tcatggtcgg cgcagaaagt ttcacatcag agacggtgaa agtggtatag ctcagaccat
     6241 agccgaacgg ataaagcgcc ccgttagctt catcaaaata acgcgaagtg tatttgttcg
     6301 gcttgtcggc attatacgga cgaccggtat tcagatggct gtagtacacc gggatctgcc
     6361 cgacagaacg cgggaaggac attggcagct tgccggacgg gttgtaatcg ccaaacaata
     6421 catcggcaat tgcattaccg ccttcagtcc ccgcaaacca ggtttccaga atcgcatcag
     6481 cctgctgatc ttctttcacc agcgccagcg gacgcccgtt catcagcacc agcaccagcg
     6541 gtttaccggt ggctttcagc gccgcaatca agtcacgttg gctttgcgga atagtgatat
     6601 cggtacggct ggaggcctcg tgcgccatcc cctgtgcttc accgaccaca gccaccacca
     6661 catcagattg tttcgccgtc tgcaccgctt catcaatcat ctcttgcggc gagcgcggat
     6721 cgactttgac cgcttcttca tactgattca ggaaatcgat aatgcctttg tcactggtaa
     6781 cgttcgcccc tttggcatac agcactttac cgttttcacc gacggcattt ttaatcccgg
     6841 tcagtacggt cacggattga tcggcaacac cggctgcgga ccagctgccc atcacgtcac
     6901 gtttactgtc cgccagtggc ccaaccaccg caatggtggc cgattttttc agcggtaacg
     6961 tttcgagacg gtttttcagc aacaccaggc tttcgcgcgc cacttcacgc gcttctttac
     7021 ggtgcaggcg gctttcggca ttggtatcca ccgggtcaga ctctttcggc cccaaatggc
     7081 tgtatgggtc gttaaacaat cccatatcat atttaacgtt cagtacatgg cgggcagcat
     7141 cgtccagctc ttccatcgtc actttgccgg atttgatcaa cccaggcaga tacttcgagt
     7201 aatactcgtc gctcatactc atgttgattc cggatttcag cgccacgcgc accgcatctt
     7261 ccgggtctgc cgccgtgcca tgtttaatca gctctttgat tgcaccgtga tcggaaacgg
     7321 tgatgccttt aaagccccac tggtcgcgca gaacatcttt cagcagccag gaatcggagg
     7381 tggctggcgt gccgttcagc gagttcagcg ccaccatcac cgcgccgctg cctgcgtcca
     7441 gccccgcttt gtacggcggc atataatcat taaacaggcg ctgcggactc atatcgacgg
     7501 tgttgtactc tttaccgcct tctaccgcgc cgtatgcggc aaagtgtttg acgctggtca
     7561 tcaccgagta gcgatctgcc gggcttttac cctgcatcgc ttccaccatg gttttaccca
     7621 ttgttgaggt gagatacgta tcttcgccaa aaccttcgga agcacgtccc cagcgcggat
     7681 cgcgcgagac atcgaccatc ggtgcccagg tcatattcag gccatcatct gccgcttcat
     7741 aagcagagac acgtccgacc gttttcactg catcgaggtt aaaagacgag gccagaccga
     7801 ggctaatcgg gaacaccgtg cgctgaccgt gcagcacgtc gtaagcaaag aaaagaggaa
     7861 ttttcaggcg gcttaattcc atcacctgat cctgcatggc gcggatatcc tgacgggtta
     7921 cggtgttgaa aatcgcccca acctgaccgt ctttgatcat ctcgcggatc gcctctttcg
     7981 ggttatccgg accgacgctg attaagcgca gctgaccaat tttctcatca actgtcattt
     8041 tcttaagcag ttcggtgacg aacgcatccc gcgcttcggg cgttaatgga tggttgccga
     8101 ataaatcatc cgccagtgct ggctgcaggg ccagactcac cgcgattcct actgaacata
     8161 gccatttcat atggattttt cttcctctgt tgcccgacga ggcagcgaaa atggtgcaaa
     8221 aaccgtagtt tgccataagc atgatggaga gagaaaaaga atgctcagtt tattgtctga
     8281 attttcaaaa tattcactcg ctgaattgtc atacaaggcg ctattctagt ttgtgatatt
     8341 ttttcgccac cacaaggagt ggaaaatgtc ttccatgaca acaactgata ataaagcctt
     8401 tttgaatgaa cttgcccgtc tggtcggtca ttcacacctg ctcaccgatc ccgcaaaaac
     8461 ggcccgctat cgcaagggct tccgttctgg tcagggcgac gcgctggctg tcgttttccc
     8521 tggctcacta ctggaattgt ggcgggtgct gaaagcctgt gtcaccgccg acaaaattat
     8581 tctgatgcag gctgccaata caggcctgac cgaaggatcg acgccaaacg gtaacgacta
     8641 tgatcgcgat atcgtgatca tcagcaccct gcgtctcgac aagctgcacg ttcttggcaa
     8701 gggcgaacag gtgctggcct atccgggcac cacgctctat tcgctggaaa aagccctcaa
     8761 accgctggga cgcgaaccgc actcagtgat tggatcatcg tgtataggcg catcggtcat
     8821 cggcggtatt tgtaacaact ccggcggctc gctggtgcaa cgtggcccgg cgtataccga
     8881 aatgtcgtta ttcgcgcgta taaatgaaga cggcaaactg acgctggtga accatctggg
     8941 gattgatctg ggcgaaacgc cggagcagat ccttagcaag ctggatgatg atcgcatcaa
     9001 agatgacgat gtgcgtcacg atggtcgtca cgcccacgat tatgactatg tccaccgcgt
     9061 tcgtgatatt gaagccgaca cgcccgcacg ttataacgcc gatcctgatc ggttatttga
     9121 atcttctggt tgcgccggga agctggcggt ctttgctgta cgtctagata ccttcgaagc
     9181 ggaaaaaaat cagcaggtgt tttatatcgg caccaaccag ccggaagtgc tgaccgaaat
     9241 ccgccgtcat attctggcta acttcgaaaa tctgccggtt gccggggaat atatacaccg
     9301 ggatatctac gatattgcgg aaaaatacgg caaagacacc ttcctgatga ttgataagtt
     9361 aggcaccgac aagatgccgt tcttctttaa tctcaaggga cgcaccgatg cgatgctgga
     9421 gaaagtgaaa ttcttccgtc cgcattttac tgaccgtgcg atgcaaaaat tcggtcacct
     9481 gttccccagc catttaccgc cgcgcatgaa aaactggcgc gataaatacg agcatcatct
     9541 gctgttaaaa atggcgggcg atggcgtggg cgaagccaaa tcgtggctgg tggattattt
     9601 caaacaggcc gaaggcgatt tctttgtctg tacgccggag gaaggcagca aagcgttttt
     9661 acaccgtttc gccgctgcgg gcgcagcaat tcgttatcag gcggtgcatt ccgatgaagt
     9721 cgaagacatt ctggcgttgg atatcgctct gcggcgtaac gacaccgagt ggtatgagca
     9781 tttaccgccg gagatcgaca gccagctggt gcacaagctc tattacggcc attttatgtg
     9841 ctatgtcttc catcaggatt acatagtgaa aaaaggcgtg gatgtgcatg cgttgaaaga
     9901 acagatgctg gaactgctac agcagcgtgg cgcgcaatac cctgccgagc ataacgtcgg
     9961 tcatttgtat aaagcaccgg agacgttgca gaagttttat cgcgagaacg atccgaccaa
    10021 cagtatgaat ccggggatcg gtaaaaccag taagcggaaa aactggcagg aagtggagta
    10081 aaaattacgg atggcatagt atcgccatcc gtcattcact taatcgttct gtgccgtctg
    10141 ccccgccgcc gccatttggg cggctttttg ttttttatag ctcaacgctg ctgctggaac
    10201 aggcatcact ttaccggttt caatccaggt acgcaggcgg ctggcatcgg caaaatgggt
    10261 atatttgcca aacgcatcca tcactaccag cgccaccggt ttattattga taaccgtacg
    10321 catcaccaga caatggcccg ccgcattggt aaaaccggtt ttggttaact gaatattcca
    10381 gttatcgcga tacaccagat ggttagtatt gcggaacggc agcgtatacg tcgggttaga
    10441 gaaggttgcc atatcttccc gggtagtact taactgcccg atcaacggat attgtttgct
    10501 ggcaatgagc agtttggtta agtcacgggc agttgaaacg ttatgcaccg acaatccggt
    10561 aggttcaaca aagcgcgtgt tgttcattcc gagcgatttc gctttcgcat tcattgcctt
    10621 aataaaggct ttgtaaccac cgggataatg gtgtgcaagg cttgccgccg cgcggttttc
    10681 tgaagacatc agcgccagca acagcatatc tttacggctg atttcgctat tcagtcgtac
    10741 gcgcgaatag acccctttca tctccggcgt ctggctgata tccactttta gtttttcatc
    10801 cagcggcagt cgtgcatcca gcacaaccat cgcggtcatt aatttgctga tagacgcaat
    10861 cggacgcacc agatccgggt ggttcgagta gatcactttg ttggtattca gatcaacaat
    10921 catcgcgcta ccggaggcaa tttcaggttg tgaagcggtg gtagcggctg ccgttttcgc
    10981 aacggcctgc ggtgccaaag gcacagccag catcagggcc aggctaaata aagaaactcg
    11041 aaatttcggc atgatgagca ttcagatagt ggttcacgcg cacaggttgc gcaccgccgg
    11101 agtaaggatt tactgaggct agcgacgcca tcataacgag caaaaagtgc gatcgtcaaa
    11161 ggagaatcgt gaggaaatgc tgcattgctg acatttacgc cagcaatgca acgtcaaaga
    11221 actttctaga acaaacgata accgtagccc catagtataa cggttagggc gagcagcact
    11281 tccagtacca gcacgccaat cgccagcgtc gaactagaga agctaaggcc ttcctctttg
    11341 ttgatattca ggaagctcgg aataccaagg taaagcaggt agccggtgta aaacagcgcc
    11401 accgtgccga ccagcgcaca caaccagacc agtggataaa gcgccaccag accgcttaaa
    11461 aacagcggag ttgcaacgta gcccgcgaag accatgcagt gcgcaagtga cggacgctgc
    11521 ggataattac gcgccatcca ccagatgacc cgccccatta ccgcaacccc agccagcata
    11581 acgccataaa acaggacagc cagcgccagt ccggtaaacc aggataactt caggatagtg
    11641 ccatcgccaa aattccagcc aatctgtgta gtgccaatga aggcgcaaat caccggaatc
    11701 gccgccatca gcaaaacgtg gtgggtgtaa tggtgagaaa tcgtttcgtt ttcgcgatta
    11761 atcacctgca tttcacgatc gggatgggaa aacagtcccc agacatggct cataccgccc
    11821 ccttgttgtg agttcatgaa cctgacagtt caagtataag tcagcttgtg attatttttt
    11881 gttcgctcat gtaatttgca ccgtctgatg tacgtccttt ttcagcgtga atgatttcca
    11941 gggtgtatgc ttacaggcaa ccaaagggag acagactggc ctatggatct caatacactc
    12001 atctcacaat atggttatgc cgcgctggtg atcggtagcc tggcggaagg tgaaaccgtg
    12061 actttgctgg gaggcgttgc ggcacatcag gggctattaa agttcccgct ggtggtactt
    12121 tctgtggcgc ttggcggcat gattggcgac caggtgcttt atctgtgcgg gcggcggttt
    12181 ggcggcaagc tgttacgccg tttctcgaaa catcaggata aaattgagcg ggcgcagaaa
    12241 cttatccaac gccatccgta tctgtttgtc attggtacgc gctttatgta tggctttcgg
    12301 gtgattggcc cgacgctgat tggtgccagc cagctgccgc cgaaaatctt tctgccgctg
    12361 aatattctcg gcgcatttgc ctgggcgttg atttttacca ctattggtta cgctggtggt
    12421 caggtgattg cgccgtggtt gcacaatctc gaccagcatt tgaagcactg ggtctggttg
    12481 attctggtcg tggttctggt ggtgggcgtg cgctggtggc tgaaacggcg cgggaagaaa
    12541 aagccggatc atcaggcgta aaaccattgc cctgggtaag gcgttcgcgc cgcatccgac
    12601 atccggtgca ccatgcctga tgcgacgctt gtgcgtctta tcatgcctac caaaatttta
    12661 cccattgaaa taaccacgca cctcactgcg ctattctggg ttgaactgcg gattcgccaa
    12721 cataaagccg ccatccacta tcaacgactg cccggtggtg taatttgcgc cctctgagca
    12781 cagccacgct accaggctgg caatctcatg cgttgcgcca aaacgccgca gaggaatcga
    12841 aggctccgcg tcgggcttca cgtcgctgtc atccatgcca ttcattggcg tggcgatcgc
    12901 cccaggcgcg actgcgttca ccaaaatctt atgcctgacc agctccagcg ccatcgtttt
    12961 ggttaacccg ccgagcgcat gtttagcggc tgtgtaagcg ctggcatccg gcagcggcgt
    13021 atgttcatgt accgacgtaa tgttgatgat acgaccgccc tgcccttgtt tcaccatctg
    13081 gcgggccgca atttgtgagc ataaaaatgc accatcgaca tcaacggtaa aaatcttgcg
    13141 ccactcatcg aaagccatat caagaaacgg cgctttggtc attgcgcccg cattattcac
    13201 cagcacatca atacgcccca gccgttgaat gagtttctcc agcgcctgcg caccttctgg
    13261 cagtttaccg agctccagct gcacgatctc cgcacgtacg ccgtggctaa ctacctcacg
    13321 cgcggtatct tttgcccctt cttcatctga gtgccaggta atgccaatat caaacccctg
    13381 ctgcgccagt aataacgcgc actctttgcc gatccccgaa tcggaggcgg taataatcgc
    13441 aacctgtgcc atcaagttct ccacttaacg ctgaataaac gttaagtata gaaggcgcat
    13501 atcatcagcg tttgtaaccc ccgcccaacg caccagtgag ttgaatagaa gcatccagcc
    13561 actgcccttg caataacagg ccattggccc gctcacgcag cgcggggatt ctggcttcgc
    13621 tgacgcggga accagcaatg atgcccgcgt taaagcgcgc ctgcgcaaga cctaccacac
    13681 gcaaggcatc gcgctcaatt tgcgcctgat gctggttttt ctccgcaagt gtctgaacct
    13741 gactggctgc ccgcgccacg tcattcaccg cttcaaccac cgctttgttg tagctggcga
    13801 tagacaagtt gctttcggct tttgcgatat tgagattggc gttaagacga ccactatcga
    13861 aaatgggtag cgtcaggcct gccgtaacgc ccatttgctg cgcggaatga cggaacagat
    13921 cgcttaagtg caacgcatcc tgttgcagga aggccatcag gttgatgtca ggataaaatg
    13981 ccgcttttgc cgcatcaatg gtgcttagcg atgactcaac gtaccagtgc gccgcctgca
    14041 aatctgcccg ccgggccagt aaggagtacc ccagttcatc aggaagctgg cttgccactt
    14101 tcggcaacgc gaccggttta agcttcaatg actttgtctg gttatttgta agtgcgctta
    14161 accgtgcctc aataattttc attttccccg cgacatcgtt gagctgctgc cgggttttgc
    14221 tggcattaat atcggtttcc acaccttcaa ctgaagaagt aatcccgttc tgatatagct
    14281 ggcgatcagt cgcgataatg gtgttctgct ctttttctat ttgctgcaag accgtgttta
    14341 acgccgcctg ggtttgccac tcccagtaca ggcgggctac gctgccagcc agcaattggc
    14401 gggtttgctc gcgttccgcc gcccgtgctt taaccgtacc caggcgggca gtaacctccg
    14461 cccgattctt tccccagata tcgagatgcc agcccgccgt taagccaaaa gtaccgttgg
    14521 tgtaccacgg gccggtcgta cctgcggccg gatcgttcag agcaaacggc cccattaagc
    14581 cttctgccga cattttttgc cgctccatat ccgccgaaaa gtcgatctgc ggaccatcct
    14641 gagtggcaac tgccttcgcc tgggcttcag ctagctgaat gcgctgttca gccacctgca
    14701 tatccggtgc gttctgtagt gcattgttaa ttaatgaagt gagctgatta tcgtgatact
    14761 ccagccacca ttggctgtct ggccaaccat ttttcagcgc cgtgggtaat gcggtgtcaa
    14821 cttgtgcagc gggcgtttgc tggcttaacg cctggcgggt ttcatgcata ggcgcacacc
    14881 cggccagcat cagtaacagc ggaaaacagg cgatggctgg ataaaaggaa tcacgattca
    14941 tgggggaata atcaggtaag aaacggtgcg cggagattac cgtgtgttgc gatatatttt
    15001 ttagtttcgc gtggcaatac atcagtagca ataaaacgac atacccagaa aaatatagac
    15061 taagtaaatg atatcttccg atttatctta atcgtttatg gataacggca aagggcttcg
    15121 ttttttccta tacttattca gcactcacaa ataaaggaac gccaatgaaa attatactct
    15181 gggctgtatt gattattttc ctgattgggc tactggtggt gactggcgta tttaagatga
    15241 tattttaaaa ttaattaatg tcatcaggtc cgaaaataac gagaatattt cagtctctca
    15301 tcctgttgcg ctcctgtcat gtgcattgct tcatataatc ac
//
LOCUS       DAZPLI010000095        15170 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZPLI010000095 DAZPLI010000000
VERSION     DAZPLI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15170
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.096"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHUU3_002799"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHUU3_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR2082819.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHUU3_002800"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHUU3_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR2082820.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHUU3_002801"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHUU3_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR2082821.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHUU3_002802"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHUU3_002802"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR2082822.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="ACHUU3_002803"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="ACHUU3_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR2082823.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="ACHUU3_002804"
     CDS             complement(8214..9107)
                     /locus_tag="ACHUU3_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR2082824.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHUU3_002805"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHUU3_002805"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR2082825.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHUU3_002806"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHUU3_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR2082826.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHUU3_002807"
     CDS             complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHUU3_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR2082827.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHUU3_002808"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHUU3_002808"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR2082828.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHUU3_002809"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHUU3_002809"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR2082829.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..>15170)
                     /locus_tag="ACHUU3_002810"
     CDS             complement(14275..>15170)
                     /locus_tag="ACHUU3_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFR2082830.1"
                     /translation="FSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQV
                     YMLDVGQGLAMVIARNGKAILYDTGRAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEH
                     LDHRGGLDSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGN
                     NHSCVVKVDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLI
                     QRVNGKVALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISS
                     LREQILPRWYHQWFGVPVDNG"
BASE COUNT         3558 a         4284 c         3741 g         3587 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttccag
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaggcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgtt atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggccc gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg
//
LOCUS       DAZPLI010000096        15054 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZPLI010000096 DAZPLI010000000
VERSION     DAZPLI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15054
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.097"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            180..422
                     /gene="yjbE"
                     /locus_tag="ACHUU3_002811"
     CDS             180..422
                     /gene="yjbE"
                     /locus_tag="ACHUU3_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR2082831.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            536..1174
                     /locus_tag="ACHUU3_002812"
     CDS             536..1174
                     /locus_tag="ACHUU3_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR2082832.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1171..1908
                     /locus_tag="ACHUU3_002813"
     CDS             1171..1908
                     /locus_tag="ACHUU3_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR2082833.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1908..4004
                     /locus_tag="ACHUU3_002814"
     CDS             1908..4004
                     /locus_tag="ACHUU3_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR2082834.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4051..4329)
                     /locus_tag="ACHUU3_002815"
     CDS             complement(4051..4329)
                     /locus_tag="ACHUU3_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2082835.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4330..4473
                     /locus_tag="ACHUU3_002816"
     CDS             4330..4473
                     /locus_tag="ACHUU3_002816"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2082836.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            4599..5009
                     /gene="psiE"
                     /locus_tag="ACHUU3_002817"
     CDS             4599..5009
                     /gene="psiE"
                     /locus_tag="ACHUU3_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR2082837.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5053..6528)
                     /gene="xylE"
                     /locus_tag="ACHUU3_002818"
     CDS             complement(5053..6528)
                     /gene="xylE"
                     /locus_tag="ACHUU3_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR2082838.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6900..7790)
                     /gene="malG"
                     /locus_tag="ACHUU3_002819"
     CDS             complement(6900..7790)
                     /gene="malG"
                     /locus_tag="ACHUU3_002819"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR2082839.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7805..9349)
                     /gene="malF"
                     /locus_tag="ACHUU3_002820"
     CDS             complement(7805..9349)
                     /gene="malF"
                     /locus_tag="ACHUU3_002820"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR2082840.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9503..10693)
                     /gene="malE"
                     /locus_tag="ACHUU3_002821"
     CDS             complement(9503..10693)
                     /gene="malE"
                     /locus_tag="ACHUU3_002821"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR2082841.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11058..12173
                     /gene="malK"
                     /locus_tag="ACHUU3_002822"
     CDS             11058..12173
                     /gene="malK"
                     /locus_tag="ACHUU3_002822"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR2082842.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLNIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12245..13585
                     /gene="lamB"
                     /locus_tag="ACHUU3_002823"
     CDS             12245..13585
                     /gene="lamB"
                     /locus_tag="ACHUU3_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR2082843.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13737..14657
                     /gene="malM"
                     /locus_tag="ACHUU3_002824"
     CDS             13737..14657
                     /gene="malM"
                     /locus_tag="ACHUU3_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR2082844.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14886..>15054
                     /locus_tag="ACHUU3_002825"
     CDS             14886..>15054
                     /locus_tag="ACHUU3_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2082845.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVTGLNRDN
                     FIYISDIFLNIK"
BASE COUNT         3758 a         3843 c         3932 g         3521 t
ORIGIN      
        1 atgattttta ttgttattta aatataagtt gaaacttata tttgatattc attccaatta
       61 tcctaaaaca ccatcgctaa ttccccgcgc cgtaattcgc atgctttagt tgtgtatact
      121 cgatcccgcc cgaaatgttt ttgggtaaat ctccattcat tcaatgaagg gaaattgtta
      181 tgaaaaaagt tctgtatggc atttttgcca tatctgcgct tgcggcgact tctgtgtggg
      241 ctgcacctgt acaggtgggc gaagcggcag gggcggcagc aacgtcggtt tcggcgggga
      301 gttcctccgc gaccagcgtc agcaccgtaa gctcggcggt gggtgtcgcg ctcgcggcaa
      361 ccggtggcgg tgatggttct aataccggga ccacaacaac cacaaccacc agtacccagt
      421 aataaagtat gtatccccaa aataattcga gtcattgcat ctgtggcttg aaatatgaag
      481 ggattaacca taaccacact ccggtgtggt tattctgccc ctctggagaa gagtcgtgaa
      541 gcgacctgca ctcattctta tctgcctgtt attacaggcc tgttcagcca cgactaaaga
      601 gctgggcaat tcactgtggg acagtctgtt cggcacgcca ggcgtacagc tgacggacga
      661 tgatattcaa aatatgccct acgccagcca gtacatgcag cttaatggcg ggccgcaatt
      721 gtttgtggtg ctggccttcg ctgaagacgg acaacaaaaa tgggtcactc aggatcaagc
      781 cactcttgtc acacaacatg gtcgtctggt gaagactttg cttggcggcg acaacctgat
      841 tgaagtgaat aaccttgccg ccgacccgct gattaaaccc gcacaaattg ttgatggtgc
      901 aacatggacg cgcacgatgg gctggaccga gtaccagcag gtacgctacg ccaccgcacg
      961 ctcagtcttc aaatgggatg gcaccgatac cgtcaaggtc ggcagcgatg aaaccctggt
     1021 tcgcgtgctg gacgaagaag tctccaccga ccaggcgcgc tggcataacc gctactggat
     1081 cgacagcgaa gggcaaattc gccagtcgga acagtatctc ggcgcggatt atttcccggt
     1141 gaaaaccact ctcatcaagg cggcaaaaca atgattaaac aaactattgt cgcgttgctt
     1201 ttgagtgtgg gagcgtcatc ggtcttcgcg gcaggaaccg tcaaggtgtt cagcaatggc
     1261 agcagtgagg ccaaaacgct aacgggcgca gagcatttaa tcgatctggt agggcaacct
     1321 cgactggcaa acagctggtg gcctggtgcg gtgattagcg aagagctggc aacggcggca
     1381 gcattgcgtc agcagcaggc gttgctgaca cggctggcag aacagggcgc agattccagc
     1441 accgacgatg ccgccgccat taacgcctta cgccagcaaa tccaggcatt ggaagtgaca
     1501 ggcaggcaaa aaatcaatct tgatcccgat atcgtccgcg ttgccgaacg cggtaacccg
     1561 ccgttgcagg gcaactacac gctgtgggtc ggaccaccgc cgtccacggt cacgttgttc
     1621 gggcttatca gccatcctgg caatcagcca ttcactccag gtcgcgacgt ggcgagctat
     1681 ctctctgacc agagcctgct cagcggtgcg gatcgcagct acgcgtgggt cgtttacccg
     1741 gacggacgca cgcaaaaagc gccggtggct tactggaaca agcgtcacgt agagccgatg
     1801 cccggcagca ttatttatgt tggcctcgca gactccgtct ggagtgagac gcctgatgcc
     1861 cttaacgccg acattcttca gactctgacg cagcggatac cccaataatg aaaaaaagac
     1921 atctgcttag cttactggcg ctgggcatta gcacagcttg ctacggcgaa acatatcctg
     1981 cgcccattgg tccgtcgcag tcggatttcg gtggcgtagg attattacaa actcccaccg
     2041 cgcgtatggc acgggaaggg gagttgagtc tgaactatcg cgataacgat cagtaccgtt
     2101 attactcagc ttcagtgcaa ctcttcccgt ggctggaaac aacgctgcgt tacaccgacg
     2161 tgcgcacccg gcagtacagc agcgtcgaag cgttctctgg cgatcaaacg tataaagata
     2221 aagccttcga tctcaaactg cgtttgtggg aagagagtta ctggctgccg caagtggcgg
     2281 ttggtgcgcg ggatatcggc ggtacggggc tgtttgatgc ggaatatctt gttgccagca
     2341 aagcctgggg gccgttcgat tttacgctcg gcctgggctg gggatatctg ggcaccagcg
     2401 gtaatgtgaa aaatccgctc tgttcagcca gtgataaata ttgctatcgc gataacagct
     2461 acaaacaagc gggatctatc gacggcagtc agatgttcca cggcccggcc tcactgtttg
     2521 gcggcgtgga ataccagacg ccctggcaac cgctgcgcct gaaactggag tatgaaggta
     2581 ataattatca gcaggatttt gccgggaagc tggagcaaaa aagtaagttt aacgtcgggg
     2641 cgatttatcg cgttaccgat tgggccgacg ttaaccttag ctatgaacgt ggcaacacct
     2701 ttatgtttgg cgtcacgctg cgcaccaact ttaacgatct gcgcccgtcc tacatcgata
     2761 acgcccgccc gcaatatcaa ccgcagccgc aggatgccat tttgcagcac tcggtggtgg
     2821 cgaatcagtt aacgttgctg aaatacaatg ccggacttgc cgatccgcag atccaggcga
     2881 aaggcgatac gctgtatgtt accggcgagc aggtgaaata tcgtgattcg cgcgaaggga
     2941 tcatccgtgc taatcggatc gtgatgaacg atctgccgga tgggatcaaa acgatccgca
     3001 ttacggaaaa tcgccttaac atgccgcagg tgacgacgga aaccgatgtc gccagcctga
     3061 aaaatcatct cgccggagag ccgttgggcc acgaaacgac gctggcgcaa aaacgcgtcg
     3121 agccagtggt tccgcagtcc accgagcagg gctggtatat cgacaaatca cgctttgatt
     3181 tccatatcga tccggtgctg aaccagtcgg tcggtggccc ggaaaacttt tacatgtatc
     3241 agctgggcgt gatgggaacg gcagatttgt ggctgacgga ccatctgctg accatcggca
     3301 gcctgtttgc aaatcttgcc aacaactacg acaagtttaa ctacaccaat ccgccacagg
     3361 actcgcactt accgcgcgtt cgtacccatg tgcgcgagta tgtgcagaac gatgcctatg
     3421 tgaataacct gcaagccaac tacttccagc atctgggcaa cggcttctac ggtcaggtct
     3481 atggaggtta tctcgaaacc atgtttggcg gtgcgggggc tgaagtgttg tatcgtccgc
     3541 tggacagcaa ctgggcgttt ggtctggatg ccaactacgt taaacagcgc gactggcgta
     3601 gtgcaaaaga tatgatgaaa ttcaccgact acagcgtgaa aaccggacat ctgaccgcct
     3661 actggacgcc atctttcgcc caggacgtgt tagtgaaagc cagcgtcggg cagtatctgg
     3721 caggggataa aggcggcacg ctggagatcg ccaaacgctt tgatagcggc gtggtggtgg
     3781 gtggctatgc cacgatcacg aatgtttcaa aagaggagta cggcgaaggg gacttcacca
     3841 aaggcgtgta tgtctcggta ccgttggatc tcttctcgtc tggcccgaca cgcagccgtg
     3901 cggcgattgg ctggacgccg ctgacgcgtg acggtggtca gcaacttggg cgtaagttcc
     3961 agttgtatga tatgaccagc gaccgtagcg tcaatttccg ctaagtcatg ggaaaggtgc
     4021 cagttttcgc aatcactggc accctcattc ttaatggcct gttgagccat caggaacgca
     4081 gttcaccccc ggtttgcgat ctggtgtgta aaggcgaaaa cattctgtct tcctgtccgt
     4141 cgatatcggt tgcaggtctg gttgcctgag cgactgggcc atcattgggc tggagacatt
     4201 gctttgcggt aggtcggctt tatcagcagt accgagcgga ccagcataag caggaagaac
     4261 agagactgat aacatcaaag cagcaaaata aggcttcatt tttaccacct ttatcaggtt
     4321 acgtttcatt tgttccagag gaacattgtc gatttttcgc gcattgctgg tggctgggaa
     4381 tcacctgaat gggtgatttt tgaattaccg gcttgggtgc ggtttgtctt gccggatgcg
     4441 ccgccaggcg cggcttatcc ggcctacggg taggtatatc cggctttggt ggaggcgcgc
     4501 tccaaatcca ggttgaacaa aacatacaca aaaaatatag atctccgtca catttttgcg
     4561 ttatacagga agctcgccac tgtgaaggag gtactgctat gacgtcactc tctcgtccgc
     4621 gcgtggagtt tatctccacc attttgcaga ccgtactcaa tcttggcctg ctgtgcctcg
     4681 gcctgatttt ggttgtcttc ctcggcaaag aaacggtgca tctggcagat gtgctgttcg
     4741 cgccagaaca aaccagcaaa tatgagctgg tagaaggact ggtggtttac tttctctatt
     4801 tcgaatttat cgcgctgatt gtgaagtact ttcagtccgg ttttcacttc ccgctacgtt
     4861 actttgtcta tattgggatc accgccattg tgcgcttgat catcgtcgat cataaatcgc
     4921 cactggatgt gttgatctac tcggctgcga tcctgttact ggtgatcact ctgtggctgt
     4981 gtaattcgaa acggctgaag cgggagtaaa aagaaagcac gccgaactgg cgcggcgtgc
     5041 tggacaggaa gattacagcg tagcagtttg ttgtgttttc ttcgtttccg gttcccagag
     5101 ggcttccagc tcctcaaggg ttttaccttt ggtttccggg acaaatttcc acataaatag
     5161 tgctgccaga acgcccatac acccgtaaat ccagtaggag aaaccgttgt ggaaatgggc
     5221 caccagccag gagtttttgt ccatcatcgg gaaggtccag gagacgaagt agttcgccag
     5281 ccactgggcc gccaccgcga ttgccagcgc tttaccacga atagcattcg ggaagatttc
     5341 cgacagcagt acccagcata ccggacccca ggacatggca aaggcagcaa catagaacag
     5401 catcgacagt agcgccacaa tacccggtgc ctgagtgtaa aacgcggtac cgaggctaaa
     5461 cataccgatt gccattccga gtgcgccgat aatttgcagt ggcttacgac caaatttatc
     5521 caccgtcata attgccagaa cggtgaaggt gaggttgata actccgacaa taatggtctg
     5581 caacagcgcg atatccgtgc tggcccccag cgttttgaac acttccggcg cgtagtacag
     5641 caccacattg atgccgacaa attgctggaa gatggagagc ataacgccga tgacaatcac
     5701 gcccacgcca aacatcagca gacgaccacc ggttttgcgg ccatgatcca gggagtgttt
     5761 aatttcctgt actgcctgag ttgcaagcgt gttgcccata attttgcgca ggatactttc
     5821 cgcctgttct tgcttgccgc gtgacatcag ccagcgagga ctttctggca cggtatacag
     5881 cagcattaag aacagcagtg cagggataca ttccgaggca aacatataac gccagccgtc
     5941 agtattcagc cagctggcat caccggaacg ggcaataaaa tagtttacgc agtaaactaa
     6001 aagttgcccg aaaataatcg caaactggtt aaatgaaacc agttttccgc gaatatgagc
     6061 tggagccagt tccgcaatat acattggtga aagcattgag gctaaaccaa cgccaatacc
     6121 gccaataata cgataaataa caaattccgg gacataacct gccagataaa taggtactgt
     6181 gttgtccggg tttatagagg taaaaccaag ttctggccag gcagaaccta caccagaaat
     6241 aaaaaacagg acagcagcaa tcttaagtga atcacgacgg ccgaagcggt tactgcaata
     6301 accaccgagg gcaccgccga tgatgcaacc aatcagagcg ctggctacgc aaaatcctaa
     6361 cagggagttg gcagcggatt cacttaagtt ttgtggagca acaaagacgg tattgagtga
     6421 ctcaacagta ccggaaataa cggcggtgtc gtagccaaat aataaaccac ctaatgtagc
     6481 gactaaggta atcgaaaata tataactgga attatactgg gtattcattc agacctgcct
     6541 tagaccattc tgatctttta tcagtgcatt gatgttggaa ttgtcaggca gttaaggcaa
     6601 ttatcatttt ttacaatgcg tttacgtaat gtcttttctg tgatcttaat tgtgataatt
     6661 atcaaaaatt gatgaaaaca atactattgc cgtgactcag agcacgaaag agaattatcg
     6721 taagtgggaa aacaaataac gtaaaaataa taagctctat gatggaaata ttaaccggcg
     6781 aacgattcag attgcagacg aaagaaaaaa aggcgctccg tggagcgccg aataacagtc
     6841 acaagttggg ataacgtaag ttgagggtgc agcggcataa cattggcaga acaacatctt
     6901 taacctttca caccacctgc cgtcaggccg ttgaccagcc agcgttgagc cagcaagaag
     6961 acgatggtga tcggtaatgc agacatcacg gcagcggcgg caaagtcacc ccacaggtag
     7021 ttttgcgggt tgaggtattg ctgcatcccc acggccaggg tgtagctgtt tacgtcacgc
     7081 agtaacagcg acgcgaccgg aacttcagta atggcagcga taaacgacag gatgaatacc
     7141 accgccagaa tcggtactga cagcggcaac aggacaaggc ggaaggcctg ccacggtgtc
     7201 gcaccatcca gcgcagccgc ttcttccagc gaactatcga tggtttcgaa atagcctttg
     7261 atggtccaga catgcagcgc aatcccaccc agatacgcga aaattacgcc gccgtgagta
     7321 ttcaggccaa tgaatggaat gtactcaccc agacgatcaa acaacgcata caacgcgacc
     7381 agtgaaagta ctgccgggaa catctggaaa atcagcattc ctttcagcag cgtcgctttg
     7441 cctggaaagc gcatacgggc gaaagcgtag gcgcaggtgg tagagagcgc gacaatgcca
     7501 atcgcggaaa tcccggcgac ctttaccgag ttccatagcc acagcagtac cgggaatggc
     7561 ggtggcgtaa tgcgaccatc agcctgttcc acgctaaaac ctaaagccag tttccagtga
     7621 tcccaggaga tttgctccgg gatcaggctg ccgatcgcaa agttcccctg gcgcagtgag
     7681 atagcgacga ccatcagcag cgggaacata atcgccgcga taaaaagtag cagtagcagg
     7741 tgagtaataa acaaacgtgc tttttgcgat ttcggttgga ccattgccat ttttgttatc
     7801 tcccttaatc aaacttcatt cgcgtggctt tcaggttcac tatcgccagc gcacccacca
     7861 gcaggaagag cagcgtggca attgctgcag ccagaccgaa gtcctgaccc ccgccgcctt
     7921 caaaagcgat acggtaggtg tagttaacaa gcaggtcggt ataaccggct ggcgtggtcg
     7981 tgccaagacg atccgggccg ccgttggtta acagttgaat cagcacgaag ttgttaaagt
     8041 taaaggcgaa gctggcgatc atcagcggcg tcagcggttt aatcagcagc ggcagcgtaa
     8101 tcttaaagaa gttctggaac ggacctgcgc catccattgc tgaggcttca tacaaatcgt
     8161 ccggaatcgc tttcagcaag cccatgcaga ggatcatcat gtacggataa cccagccagg
     8221 tattgacgat aattagcatc gtgcgggcgg tggtcggatc gctgaaccag gcgggcttca
     8281 cgccaaacag cgcgctcaac atcatgttga tttcaccgaa gctctggtta aacaaccctt
     8341 tgaaaatcaa gattgaaatg aatgatggca ccgcgtaggg cagaatcagc agaacgcgat
     8401 agaccgcttt gccgcgcaat gcttcccact gcaccagaca tgccagaacc atgccgaccg
     8461 ccaccgttaa aaagacagtg atcagcgaga acaccacggt ccagacgaaa atggcgagga
     8521 acggtttctg aatgccttcg tcggtgaaga cgcgggtaaa gtttttccag ccagtagtca
     8581 cggtgtaacc ggggcttagc ttttcatcac cccagttgcc gtcggcggta atggactggt
     8641 aaaagccaat ttggttattc ggacgatatt tcacgccgct ctgattattc gtcaacgtgc
     8701 cgtcaccgtc gagtgtgtag agcggctgcg tgccagaaaa ctggcgcagg gagctcatca
     8761 tcactttgtt gccatccggc agaatggcgg taatgtcact cagcgcctga cgattctggg
     8821 taatcacgcg tagattcgcg cgttcgcctt cgggctgggc ggtcgtttct ttcagttgca
     8881 gtttttgttc gccgccaaat ttaaacgcgt cggagaggta atttttgccg atttcgccgt
     8941 cactgagcgc cagttgccac tcatcgcccg ccgggtaaag accaaagtta taggttttgc
     9001 ctgcttgcca ggagcgatct aacaacactt cctgcgcaca ttcaaaagtc agctggttag
     9061 tgctgctgta gttggtgaag gcaatggcga tggtgcagac cagagggaag aggacgaata
     9121 atcccattcc agccattccc gggtaaacat agcgccaggc gtaggcttta cgattggcga
     9181 aaatatacag ccccgctgaa ctcaatatca gcgtggtaat ggcgaacagg tattcccctt
     9241 gtgcgtacat taaaacaaca aggtaaccca ccagcaggcc gagcagacct agcactgacc
     9301 atttcagcgc gtcgctttgc caccaatgtt tctttttaat gacatccatg gggttcttcc
     9361 tcattccagg acggataagg ctttcacgcc ttatccgaca acaactgcct gatgcgacac
     9421 tgatgcgtct tatcaggcct acatgcgttt cagttttgta ggccggacaa ggcgttcacg
     9481 ccgcatccgg catttcacag cattacttgg tgatacgagt ctgcgcgtct ttcagggctt
     9541 catcgacagt ctgacgaccg ctggcggcgt tgatcaccgc agtacgcacg gcataccaga
     9601 aagcggacat ctgcgggatg ttcggcatga tttcaccttt ctgggcgttt tccatggtgg
     9661 cggcaatacg tggatctttc gccaactctt cctcgtaaga cttcagcgcc actgcaccca
     9721 gcggtttgtc tttattgacc gcttccagac cttcgtcagt cagcagatag ttttcgagga
     9781 actctttcgc cagctctttg ttcggactgg cggcgttaat accggcgctc agcacgccaa
     9841 cgaacggttt agatggttga cccttgaagg tcggcagtac cgttacacca taattcactt
     9901 tgctggtgtc gatgttggac catgcccacg ggccgttgat ggtcatcgct gtttcgcctt
     9961 tattaaaggc agcttctgcg atggagtaat cggtgtctgc attcatgtgt ttgtttttaa
    10021 taaggtcaac caggaaggtc agacccgctt tcgcgccagc gttatccacg gccacgtctt
    10081 taatatcgta cttgccgttt tcatacttga acgcataacc cccgtcagca gcaatcagcg
    10141 gccaggtgaa gtacggttct tgcaggttga acatcagcgc gctcttacct ttcgctttca
    10201 gttctttatc cagcgccggg atctcttccc aggtttttgg tgggttcggc agcagatctt
    10261 tgttataaat cagcgataac gcttcaacag cgatcgggta agcaatcagc ttgccgttgt
    10321 aacgtacggc atcccaggta aacggataca gcttgtcctg gaacgctttg tccggggtga
    10381 tttcagccaa caggccagat tgagcgtagc caccaaaacg gtcgtgcgcc cagaagataa
    10441 tgtcagggcc atcgccagtt gccgcaacct gagggaattt ctcttccagt ttatccggat
    10501 gctcaacggt gactttaatt ccggtatctt tctcgaattt cttaccgact tcagcgagac
    10561 cgttatagcc tttatcgccg ttaatccaga ttaccagttt accttcttcg attttggcga
    10621 gagccgaggc ggaaaacatc atcgtcgtta atgcggataa tgcgaggatg cgtgcacctg
    10681 tttttatttt cataatctat ggtccttgtt ggtgaagtgc tcgtgaaaac acctaaacgg
    10741 actctagttt ctttatacgg caacctcttt ccatcctcct tgcccctacg ccccaccgtt
    10801 gctttgtgtg atctctgtta cagaattggc ggtaatgtgg agatgcgcac ataaaatcgc
    10861 cacgattttt gcaagcaaca tcacgaaatt ccttacatga cctcggttta gttcacagaa
    10921 gccgtgttct catcctcccg cctcctcccc cataaaaaag ccagggggtg gaggatttaa
    10981 gccatctcct gatgacgcat agtcagccca tcatgaatgt tgctgtcgat gacaggttgt
    11041 tacaaaggga gaagggcatg gcgagcgtac agctgcaaaa tgtaacgaaa gcctggggcg
    11101 aggtcgtggt atcgaaagat atcaatctca atatccatga aggtgaattc gtggtgtttg
    11161 tcggaccgtc tggctgcggt aaatcgactt tactgcgcat gattgccggg cttgagacga
    11221 tcaccagcgg cgacctgttc atcggtgaga aacggatgaa tgacactccg ccagcagaac
    11281 gcggcgttgg tatggtgttt cagtcttacg cgctctatcc ccacctgtca gtagcagaaa
    11341 acatgtcatt tggcctgaaa ctggctggcg caaaaaaaga ggtgattaac caacgcgtca
    11401 accaggtggc ggaagtgctg caactggcgc atttgctgga tcgcaaaccg aaagcgctct
    11461 ccggtggtca gcgtcagcgt gtggcgattg gccgtacgct ggtggccgag ccaagcgtat
    11521 ttttgctcga tgaaccgctc tccaacctcg atgctgcact gcgtgtgcaa atgcgtatcg
    11581 aaatctcccg tctgcataaa cgcctgggcc gcacaatgat ttacgtcacc cacgatcagg
    11641 tcgaagcgat gacgctggcc gacaaaatcg tggtgctgga cgccggtcgc gtggcgcagg
    11701 ttgggaaacc gctggagctg taccactatc cggcagaccg ttttgtcgcc ggatttatcg
    11761 gttcgccaaa gatgaacttc ctgccggtaa aagtgaccgc caccgcaatc gatcaagtgc
    11821 aggtggagct gccgatgcca aatcgtcagc aagtctggct gccagttgaa agccgtgatg
    11881 tccaggttgg agccaatatg tcgctgggta ttcgcccgga acatctactg ccgagtgata
    11941 tcgctgacgt catccttgag ggtgaagttc aggtcgtcga gcaactcggc aacgaaaccc
    12001 aaatccatat ccagatccct tccattcgtc aaaacctggt gtaccgccag aacgacgtgg
    12061 tgttggtaga agaaggtgcc acattcgcta tcggcctgcc gccagagcgt tgccatctgt
    12121 tccgtgagga tggcactgca tgtcgtcgac tgcataagga gccgggcgtt taagcacccc
    12181 acaaaacaca caaagcctgt cacaggtgat gtgaaaaaag aaaagcaatg actcaggaga
    12241 tagaatgatg attactctgc gcaaacttcc tctggcggtt gccgtcgcag cgggcgtaat
    12301 gtctgctcag gcaatggctg ttgatttcca cggctatgca cgttccggta ttggctggac
    12361 aggtagcggc ggtgaacaac agtgtttcca gactaccggt gctcaaagta aataccgtct
    12421 tggcaacgaa tgtgaaactt atgctgaatt aaaattgggt caggaagtgt ggaaagaggg
    12481 cgataagagc ttctatttcg acactaacgt ggcctattcc gtcgcgcaac agaatgactg
    12541 ggaagctacc gatccggcct tccgtgaagc aaacgtgcag ggtaaaaacc tgatcgaatg
    12601 gctgccaggt tccaccatct gggcaggtaa gcgcttctac caacgtcatg acgttcatat
    12661 gatcgacttc tactactggg atatttctgg tcctggtgcc ggtctggaaa acatcgatgt
    12721 tggcttcggt aaactctctc tggcagcaac ccgctcctct gaagctggtg gttcttcctc
    12781 ttttgccagc aacaatattt atgactatac caacgaaacc gcgaacgacg ttttcgatgt
    12841 gcgtttagcg cagatggaaa tcaacccggg cggcacatta gaactgggtg tcgactacgg
    12901 tcgtgccaac ctgcgtgata actatcgtct ggttgatggc gcatcgaaag acggctggtt
    12961 attcactgct gaacatactc agagtgtcct gaagggcttt aacaagtttg ttgttcagta
    13021 cgctactgac tcgatgacct cgcagggtaa aggtctgtcg cagggttctg gcgtcgcgtt
    13081 tgataacgaa aaatttgcct acaatatcaa caacaacggt cacatgctgc gtatcctcga
    13141 ccacggtgcg atctccatgg gcgacaactg ggacatgatg tacgtgggta tgtaccagga
    13201 tatcaactgg gataacgaca acggcaccaa gtggtggacc gtcggtattc gcccgatgta
    13261 caagtggacg ccaatcatga gcaccgtgat ggaaatcggc tacgacaacg tcgaatccca
    13321 gcgcaccggc gacaagaaca atcagtacaa aattaccctc gcacaacaat ggcaggctgg
    13381 cgacagcatc tggtcacgcc cggctattcg tgtcttcgca acctacgcca agtgggatga
    13441 gaaatggggt tacgactaca acggcgatag caaggttaac ccgaactacg gcaaagccgt
    13501 tcctgctgat ttcaacggcg gcagcttcgg tcgtggcgac agcgacgagt ggaccttcgg
    13561 tgcccagatg gaaatctggt ggtaatagca aaacctgggc cggataaggc gtttacgccg
    13621 catccggcat aaaaacaggt tgtcattatc tgaaaggggc gaaagcccct ctgattatcg
    13681 ggtttagcgc gctattgcct ggctaccgct gagctccaga ttttgaggtg aaaacaatga
    13741 aaatgaataa aagtctcatc gccctctgtt tatcagcagg gttactggca agcgcgcctg
    13801 gaattagcct tgccgatgtt aactacgtac cgcaaaacac cagcgacgcg ccagccattc
    13861 catctgccgc gctgcaacaa ctcacctgga caccggtcga tcaatctaaa acccagacca
    13921 cccaactggc gaccggcggc caacaactga acgttcccgg catcagtggt ccggttgctg
    13981 cgtacagcgt cccggcaaac attggcgaac tgaccctgac gctgaccagc gaagtgaaca
    14041 aacaaaccag cgtttttgcg ccgaacgtgc tgattcttga tcagaacatg accccatcag
    14101 ccttcttccc cagcagttat ttcacctacc aggaaccagg cgtgatgagt gcagatcggc
    14161 tggaaggcgt tatgcgcctg acaccggcgt tggggcagca aaaactttat gttctggtct
    14221 ttaccacgga aaaagatctc cagcagacga cccaactgct cgacccggct aaagcctatg
    14281 ccaaaggcgt cggtaactcg atcccggata ttcccgatcc ggttgctcgt cataccagcg
    14341 atggcttact gaaactgaaa gtcaaaacga actccagctc cagcgtgttg gtaggaccac
    14401 tatttggttc ttccgctcca gctccggtta cggtaggcaa cacggcggca ccagctgtgg
    14461 ctgcacctgc tccggtgccg gtgaagaaaa gcgagccgat gctcaacgac acggaaagtt
    14521 attttaatac cgcgatcaaa aacgctgtcg cgaaaggtga tgttgataag gcgttaaaac
    14581 tgcttgatga agctgaacgc ctgggatcga catctgcccg ttccaccttt atcagcagtg
    14641 taaaaggcaa ggggtaatta cgccccacag tgctgatttt gcaacaactg gtgcgtctcc
    14701 tggcgcacct ttttttatgc ttccttcctg ggatatgagc gattttttat agtacttcac
    14761 ttcttcttca ctaagaatgt ccattatctc aatgccttat cagagattct tttcctttcg
    14821 ccggtagtgt ctggacgctc aggctacttt tccaggttat tttatttctg tattacagag
    14881 gttttatgat aggtcatatc acaaattctg gcggcaataa ctctttgatg aaacatgatg
    14941 tggttcaagg aaataatata gtagatcttg atttactacg taatttcaat ggggtgacag
    15001 gtttaaacag agataacttt atttatatca gtgatatatt tttaaatata aaac
//
LOCUS       DAZPLI010000097        14886 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZPLI010000097 DAZPLI010000000
VERSION     DAZPLI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.098"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            261..1559
                     /gene="kgtP"
                     /locus_tag="ACHUU3_002826"
     CDS             261..1559
                     /gene="kgtP"
                     /locus_tag="ACHUU3_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR2082846.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1556..1879)
                     /locus_tag="ACHUU3_002827"
     CDS             complement(1556..1879)
                     /locus_tag="ACHUU3_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR2082847.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1925..3280)
                     /gene="pssA"
                     /locus_tag="ACHUU3_002828"
     CDS             complement(1925..3280)
                     /gene="pssA"
                     /locus_tag="ACHUU3_002828"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR2082848.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3394..6054)
                     /gene="pat"
                     /locus_tag="ACHUU3_002829"
     CDS             complement(3394..6054)
                     /gene="pat"
                     /locus_tag="ACHUU3_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR2082849.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6086..6784)
                     /gene="tapT"
                     /locus_tag="ACHUU3_002830"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6086..6784)
                     /gene="tapT"
                     /locus_tag="ACHUU3_002830"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR2082850.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6853..7272)
                     /gene="trxC"
                     /locus_tag="ACHUU3_002831"
     CDS             complement(6853..7272)
                     /gene="trxC"
                     /locus_tag="ACHUU3_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR2082851.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7479..8516
                     /locus_tag="ACHUU3_002832"
     CDS             7479..8516
                     /locus_tag="ACHUU3_002832"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR2082852.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8564..9253)
                     /gene="ung"
                     /locus_tag="ACHUU3_002833"
     CDS             complement(8564..9253)
                     /gene="ung"
                     /locus_tag="ACHUU3_002833"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR2082853.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9558..9941
                     /gene="grcA"
                     /locus_tag="ACHUU3_002834"
     CDS             9558..9941
                     /gene="grcA"
                     /locus_tag="ACHUU3_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR2082854.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(9997..10584)
                     /gene="eamB"
                     /locus_tag="ACHUU3_002835"
     CDS             complement(9997..10584)
                     /gene="eamB"
                     /locus_tag="ACHUU3_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR2082855.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10687..11568
                     /locus_tag="ACHUU3_002836"
     CDS             10687..11568
                     /locus_tag="ACHUU3_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR2082856.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11777..13111)
                     /gene="srmB"
                     /locus_tag="ACHUU3_002837"
     CDS             complement(11777..13111)
                     /gene="srmB"
                     /locus_tag="ACHUU3_002837"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR2082857.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13243..13980
                     /gene="trmN"
                     /locus_tag="ACHUU3_002838"
     CDS             13243..13980
                     /gene="trmN"
                     /locus_tag="ACHUU3_002838"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR2082858.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13965..>14886)
                     /locus_tag="ACHUU3_002839"
     CDS             complement(13965..>14886)
                     /locus_tag="ACHUU3_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFR2082859.1"
                     /translation="VANLEFNQFPPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFM
                     PDFDERGELAPRDIVARAIDHEMKRLGADCMVLDISHKPADFIRQHFPMIYEKLLGLG
                     IDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLE
                     CLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVG
                     IVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKES
                     RGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3588 a         3768 c         3899 g         3631 t
ORIGIN      
        1 gatgcgacac tgcgcgtctt atactcccac atatgccaga ttcagcaacg gatacggttt
       61 ccccaacttg cccacttcca tacgtgtcct ccttaccaga aatttatcct taagctcctc
      121 aataaccatt ttcctgctaa ctaaattcat ggttaaggtt gcacaatgat atgcaacaaa
      181 tgtataacat ttcctttacc aaaaaaaata aacaaaagcg accgacaaaa gcatcggatt
      241 acggcaggag acataatggc atggctgaaa gtactgtaac ggcagacagc aaactgacaa
      301 gtagtgatac tcgtcgccgc atttgggcaa ttgtgggggc ctcttcaggt aatctggtcg
      361 agtggttcga tttctatgtc tactcgttct gttcactcta cttcgcccac atcttcttcc
      421 cttccgggaa cacgacgact caactactac aaacagcagg tgtttttgct gcgggattcc
      481 tgatgcgccc aataggcggt tggctatttg gccgcatagc cgataaacat ggtcgcaaaa
      541 aatcgatgct gctatcggtg tgtatgatgt gtttcggctc actggtaatc gcctgtttgc
      601 ctggttatga aacaatcggt acgtgggctc cggcattatt gcttctcgcg cgtttatttc
      661 agggattatc cgttggcgga gaatatggca ccagcgccac ctatatgagt gaagttgccg
      721 ttgaagggcg caaaggtttt tacgcatcat ttcagtatgt gacgttgatc ggcggacaac
      781 tgctagccct actggttgtc gtggttttac aacacaccat ggaagacgct gcactcagag
      841 agtggggatg gcgtattcct ttcgcgttag gagctgtgtt agctgttgtg gcgttgtggt
      901 tacgtcgtca gttagatgaa acttcgcaac aagaaacgcg cgctttaaaa gaagctggat
      961 ctctgaaagg attatggcgc aatcgccgtg cattcatcat ggttctcggt tttaccgctg
     1021 cgggctccct ttgtttctat accttcacta cttatatgca gaagtatctg gtaaatactg
     1081 cgggaatgca tgtcaacgtg gcgagtggca ttatgactgc cgcattgttt gtattcatgc
     1141 ttattcaacc actcattggc gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat
     1201 gtttcggttc gctggcagcc atttttaccg ttcctattct ctcagcattg caaaacgttt
     1261 cctcgcctta tgccgctttt ggtctggtga tgtgtgccct gctgatagtg agtttttata
     1321 catcaatcag tggaatactg aaggctgaga tgttcccggc acaggttcgc gcattaggcg
     1381 ttggcctgtc atatgcggtc gctaatgcta tatttggtgg ttcggcggag tacgtagcgt
     1441 tgtcgctgaa atcaatagga atggaaacag ccttcttctg gtatgtgacc ttgatggccg
     1501 tggtggcgtt tctggtttct ttgatgctac atcgcaaagg gaaggggatg cgtctttagt
     1561 gacgggtcag ttgccagacg gtatagccgg tgcttgcacc ggcgacatcc caggccaaat
     1621 ccttccagct ccagccgctc ccttcggggc ggctatccca aagctctttt gacgccccca
     1681 aactgacaga gaacatcaat ccaaacatgg cactgcgatc ccggctcatc ccctgatgct
     1741 gtgaatattc atttccggcg gcggaaagca tcgccgaggc gataaagtgt tgagctttat
     1801 cctgcccgct ccagctatcg ttagccatat ggctgcatcc agaaagtaac aatagcgaac
     1861 agacaaaaag aatacgcaaa acgtggcctc caaaaaacaa accccgtaca ggacggggtt
     1921 gtgattacag gatgcggcta attaatcggt cgatgcggat acggcgcaaa cggcggatga
     1981 gtttacgaac cttcaccgga taatcggcaa tactttgcag atcgcgatag tgcttaacga
     2041 tggtggtatg ctcgcggatc agctccagtt ctttctctcg ctgcggcgca agcttcagtt
     2101 gtggatcgtg gatcaaaatg gcgttttcca gatccagacg ccaggcgcgc gggttcaggt
     2161 tattaccggt gatcaacatc cacttatcat caacccacat ccctttcagg tgataggtgt
     2221 tgtcgtcatc tttccataac cgaaccacta gctggtcagt attgacgtaa tactgcaaac
     2281 ggctcaggaa acgacgcaga ttgatctcat agagataagg caatgcgcca attatcttga
     2341 aaggttcatc ttccggaatg tagaagtcgt tcgcggtttt atcaccaaca ataatttcga
     2401 cctttttccc ttcgcgcagc aactggataa tattgcgcac aaggattgct ggcaggttga
     2461 agtatggcgt acagatggtt agtttctgct cggcacaagg cataagatgg aaaatggtct
     2521 tgttcaacag actcgatttc cccagcccca ctagcggcgt tacggaaagc tgatcgttgt
     2581 cggcatcgcc ctggaaatga taagcggcat cacgcagctc ctggcggaac agacgaatat
     2641 cgttcttgat ttccgggctt tttggccgat taacatcatc cagacgatta acgccgcggc
     2701 cattcataat attctgtgta acccattcaa acataatgtc tgacatctta cggttacgga
     2761 taagatgata acggtcgtag cgatatttat cgtgctgatg caggtaaaca tcgttcaggc
     2821 tggcaccgct ataaagtacg ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa
     2881 gggcttcacg ggtattgatt ggaacgccat aaaccggaac atctacgccc gaattttcct
     2941 gcgccatgcg gcagtaccag tcagcgttag tgttagatgc cgcagcgcca atgcgtccac
     3001 gttgtgcacg atgccagtcg accagcaccc gcacatccag ttccggacgc tgccttttag
     3061 cttcatacaa cgcgttcaga atgcctttgc caccgtcatc ctgttcgaga tacagggcga
     3121 caatgcaaat gcgctgcttc gcgctggcta ttttttccag cagcgtctcc cggaagtcgg
     3181 cgggagcgta aaagaaatcg acatcatcaa ctgattgaga aatcttgggt agttgggcaa
     3241 ggtgttgttg atgtttatta cgcttaaatt ttgacaacat cacagtgcat ttcttctctg
     3301 ttcattgaag ggtcctctgt gcaatgcaga cgacataagc gggcaataat aacaccagtc
     3361 ccgattaagt ggtcaacatt tccagtacct tactcatgat tcctcgcgtt gggcaagatt
     3421 tagcgtaagc ccaacgatcc cctcttcgag ctggatatca acgttaaacc cgagcttgcg
     3481 ggctagcgcc accatgccac gattgtttgg catcgtaata ccattcagac gttgtagtcc
     3541 gtgatctcgc gtataggtaa tcaacttttc cattaagcgt cgacctaagc ctaacccttt
     3601 gagatccgag cgaaccagca cagcaaattc ggcatcgatg ttatcaggat cggaaatcgc
     3661 acgcgtgacg ccgaggatct cttccgtttg atcaatacgt cgtaccgcta caaacgccat
     3721 ttcccgatcg tagtcgatct gtgtcatgtt ggctaaatct tcatgggtaa attcgttgat
     3781 ctcgctaaag tagcggtaat aaagatcttc tttggtgact cgcgaaatga attgctgaag
     3841 ttgtggctca tcttctggca aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc
     3901 tacccattct tccagctgat gcggataagg gcgcactgcc agccgactct cgttatcgcc
     3961 ttcaaacggc gagatatcca gcgtgacatc cagcgcggta aattcactgc cagaagccag
     4021 caaaggatga atatccagac gctgaatttc cgggcaatcg acaatcaagt tggaaacctg
     4081 caccagaagc tggctcaagc ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat
     4141 ctttttactt ttgatcccct gaataaccag atagcgggcc aggttcatgt tcagcggcgg
     4201 cagtgcgacg acggcttgat cttcaggacg ccactccaca ccgccttcac ccagcatgat
     4261 caacggcccg aaaaccggat cgtgctcaac cacaacccgc aactcctgag cgccagcacg
     4321 gttagccata ctttgcacca acaggccgtg gacccgcgcc tgtggccagg ccatttttac
     4381 gcgatcgaaa atagcgttcg ccgcttgctg gacttcattg gctgtacgca ggtaaagcat
     4441 gacgccctga acttccgatt tatgtggaat atccggcgaa cgcaatttca gcgccaccgg
     4501 ataaccaatc tgttcggcaa tatgcaccgc ttcggtgcta tcgctggcaa tccaggtagg
     4561 gagcgtgttc atgccatacg cttgcaggat gggctgaact tcatgggtat cgagcgacgt
     4621 agccccttcg gcaatcgcct gttgcaacag aagatgcgct tctgcggtat tggaagtcag
     4681 attgctgggc aacgtcggcg tttcgcgtag ttgcttctga ttacgccggt actccaccat
     4741 atgcataaaa gcagtgatgg ttccttccgg ggtacggtag gtcggcagcc cggcttcgct
     4801 gaataaacgt cgtgcctctt gcgaggagtg ctcgccgcac cagttcgtca gcaaagagac
     4861 atatttgctg cggggatgat gctttaccgc ttcaattaat acttgcgcgc tttctgttgc
     4921 gggagcagcg gcgctgggcg aatgaataac catcagcgca tcaaaatcct ggctgtggag
     4981 cagaatgtcc agcgttttga cataatgctc gctgctggcg tcatcgcgca gatcgagagg
     5041 gttagagact gccacatgtt ctggcaatgc atcgcgcaat ttctggcagg tttcttcgct
     5101 tagcgttgcc agcttgccat tgcgtgacca taaggcatcc agcgccagcg cggcaggcgc
     5161 agcaccgttg ctgataatca tcaaccggtc gccacgcagc gggcgcatat ggctaagggt
     5221 ttccaccgcc gaaaacagtt cgtgggtatc ctgtacccgc aacaaaccgg cacgctgaat
     5281 agccgcatcc catgccgggt ccattcctgc cgtcgtgttg agcagtcgct gtgccgccgg
     5341 gctacgtccg cttttaatca ccagaatcgg tttattacgc gaggcactac gggctgccga
     5401 aacaaagcgt cgcgcgtcgc ttaactgttc gagatagagc aggatggcgc tggttttact
     5461 gtcgcgcgcc agatagtcga gcaattcatc aacgtcgata tccaggctgt cgccgagcgc
     5521 aataaagtag gaaaagccca tctcacgctg ttgcgcccag tcgaggatgg tgttggagac
     5581 ggctgccgat tgtgaaataa acgccagctt gccgcgttta atcggcacag gcgaaaagct
     5641 ggcattcaga ccttgccagg gagccagtaa acccagactg tttggtccaa gcaggcgcat
     5701 gttatggcgc agggcgcagg cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat
     5761 aatgcaggtt ttacagcctt tctcgccgag ctcttccaga agagcaagat tacggctggc
     5821 attggtacat aaaaccgcaa ggtcgggtgt aaagggcaag ctggcaatat ccggccaggc
     5881 caacacaccc aacaccgctt tccaggctgg cgtcaccggg agtaccggtc cgttaaagct
     5941 tcccgccagc aggttacgca tcatcaggta acctgcgcga ttgggtttca tcgacgcgcc
     6001 aattaccgct atcgattttg gtcgcagtag tgcttccagt cctcgctgac tcataccggt
     6061 ctcccgcaga agtgaccgaa tgattttaaa cgctttctaa ctgttctgct gtgatgctac
     6121 ccagatgttg cgtttttcct gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta
     6181 accctgctgc cgccccggta tcgccggcca tatctaacag tgcgatggct acctcggcag
     6241 tacaatattg gcctgcagcc tgggcttcac gcaggcgata ggcagaaagc cgggaaagat
     6301 cgacggaaat gacgggaaga ttatccagat acggactttt acgaaacatc ttgcgagctt
     6361 ccggccaggt accatcgagc atgataaaca gcggtggctt accggcaggt ggtgtgaaga
     6421 tcacttcccg ttgctcatca gcatacgagg cgggaaagac caccattggc tgataatccg
     6481 ggttttgtac cagatccagc aaatcctgcg agggttcggt acgcgaccat tgaaacgcaa
     6541 cggtatcagg caaaatatca gcaatcagac gcccggtatt actgggcttc attggctcgg
     6601 tgtcgaacat cagcaaacag aagcgacttt ttgcttgtgc tggggtaatt gtcgaacaga
     6661 gacataattt ctctggcaaa agacagcgtt ggcagcgatg aacgcgatta ccgcgggcaa
     6721 gaaaaggacg tgttgcgcgc gcaatacgct cggcgcgtaa ctggagaaca gcgttttcgg
     6781 tcataagaga gcgtcgaaaa aacgccattg tcgcagagga gaaaacgggg cacaagatgc
     6841 accccggtaa gattaaagag attcgttcag ccagctatcg aacggcgctt tcggtactgc
     6901 gccattaagc atgtcgacaa cctgaccgtt tttgaaaatc atgatcgtcg gtatactacg
     6961 aattccaaag cgactgctca attcacgttc agcttcggta ttcactttca caaagcgcac
     7021 tttaccgcta cgctcttgcg cgacatcttc aaaaattggt gcgaaattac ggcaggggcc
     7081 gcaccacggt gcccagaagt cgatcaccac aggtagatca tccttcagca atttgtcgag
     7141 tgtttcaccg gtcgcattaa tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca
     7201 ttttgccgca tcttcgatcc gatcgtcggg aatgcgattg atggcctgac aatgggtaca
     7261 aacggtattc ataactaacc tcgggatgag taaggagact tatcggcaac gacgccggta
     7321 tgtttctaat atgttacata ttatcgggga cattgttcca gacaacaatc tgttttattc
     7381 aataggtatg atagtcgcag gctttggtac gaaatcatgg cgaaccgcgc ggacttcggg
     7441 taatctgcgc gcttcgcgca gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta
     7501 aaagcggcaa ggtcaaagtg atgtatgtcc gcagtgatga tgattctgat aaacgtaccc
     7561 acaacccgcg taccgggaaa gggggcgggc gtccaggaaa atctcgtgct gacggtggcc
     7621 gtcgccccgc ccgcgatgac aaacagagtc agccccgtga ccgcaagtgg gaagattcgc
     7681 cgtggcgcac ggtttcccgc gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg
     7741 gcatcagtgg taaaagtttt attgatccgg aagtgttgcg tcgtcagcgt gcggaagaaa
     7801 cccgcgtcta cggcgaaaat gcctgtcagg cactgttcca gagccgtccg gaagcaattg
     7861 ttcgcgcctg gtttatccag agcgtaacgc cacgttttaa agaagccttg cgctggatgg
     7921 cggcaaaccg caaagcgtac catgtggtgg atgaagctga attgacaaaa gcgtcaggca
     7981 cggaacatca cggcggcgtt tgcttcctga tcaaaaagcg taacggtaca accgtgcagc
     8041 agtgggtaag ccaggcaggc gcgcaggatt gcgttctggc actggaaaat gaatctaacc
     8101 cgcataacct gggtggcatg atgcgcagct gcgcgcactt cggcgtgaaa ggtgtcgtgg
     8161 tgcaggatgc ggcactgctg gagtcggggg cggctatccg taccgcagaa ggcggcgcag
     8221 agcacgttca gccgattact ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg
     8281 caggttacac cgtagtgacc acttccagcg agcagggtaa accgctgttc aaaactagtc
     8341 tgccagcgaa aatggtactg gtgctgggtc aggaatatga agggttaccg gatgccgcac
     8401 gcgatccgaa cgatctgcgc gtgaagattg acggtacggg caacgttgcc gggctgaata
     8461 tctctgtcgc aaccggcgtt ttgcttggtg aatggtggcg tcagaataaa gcctgataaa
     8521 tcagccgggt ggcaactctg ccatccggca tttccccgca aatttactca ctctctgccg
     8581 gtaatactgg catccagtca atcggcgtct cgccacgttg ttccagccac tgatttgcca
     8641 gcacaaaatg gttgcagcca aagaatccac gatgcgccga aagcggcgac ggatgcggtg
     8701 ctttcagtac atgatggcgt tgcttatcta taatcgcccc tttcttttgc gcatgtgatc
     8761 cccacaacaa aaacaccacg ccttcgcggt gctggttaat caggctgatg actttatcag
     8821 taaacgtctc ccaaccgagg ctggcgtggg aatgcgcctg acctgcgcga accgtcaaca
     8881 cagtattgag cagcagaacg ccctgacgcg cccagctttc gagatagcca tgattagggc
     8941 gggtgaagcc cggaatagta ttttccagct ctttatacat attcaataat gacggaggaa
     9001 tggcaatgcc gggacgaacg gaaaatgcca gaccatgcgc ctgtcccggt ccgtgataag
     9061 gatcctggcc gagaatcacc actttaacgt cacccaactc ggtaaagcgg aacgcgttaa
     9121 agacatcttt ttgtggtgga tagatagtga cgccggactg ccgctcgctg gcgacggtct
     9181 gaagggtatt aagaaaataa ggttgttgct tctcttcagc cagcacgtca tgccaggtta
     9241 attcgttagc catctcgctc tcctgcgaat cttcaatccg cctagcttaa ctgcttcttt
     9301 ctacagaaca aaatcatgct cattttttag acttaaacga gtgcaaaaaa atgtaaattg
     9361 gtaaaatttc acacccgcat aagttgatgt aaaacaataa aatccattta cgaccacaat
     9421 cgatgtgtgg tttttattga tttaaatcaa agaatcaagg gtggttgagg agtatatata
     9481 cactcaagca acaatggttt taccaattgg ccgcgacagg ctgaacaaat caaataattt
     9541 tgccggggag gcatcacatg attacaggta tccagattac taaagccgct aacgacgatc
     9601 tgctgaactc tttctggctg ctggacagcg aaaaaggcga agcacgttgc atcgttgcaa
     9661 aagcaggtta tgcagaagat gaagtggttg cagtaagcaa actgggtgac attgaatacc
     9721 gtgaagttcc agtagaagtg aaaccagaag ttcgcgttga aggtggtcaa cacctgaacg
     9781 ttaacgttct gcgtcgtgaa actctggaag atgcagttaa gcatccggaa aaatatccgc
     9841 agctgaccat ccgtgtatcc ggttatgcag ttcgctttaa ctctctgact ccggaacagc
     9901 agcgcgacgt tatcgctcgt acctttactg aaagcctgta aggcttaagt agcaagttac
     9961 taagcggaag agggcgacct cttccgcttt ttttcgttaa tagaaaatgc gtaccgcgca
    10021 atagaccagt aacagggcaa gcacgatatt taactggcga ccatactggc gaaacaatcg
    10081 ctgaaacaga tgccccgcca gcgcccagca cacattgcca aacgtcccaa tcatcgccag
    10141 caaaacgctg acgccaacta cccagcttag cgcctgtgtt tgcggcagaa caaacgtcga
    10201 cagtgccgta acaccgtaca aaatgatttt gacgttcaca aactgcaaag caaagctggc
    10261 ccaaaagctg attggttttg cctgaagtcc gtcttccttt gttgggctgg tggcgatttt
    10321 ccacgccagc cagacaatat atgccgcccc cgcccaactc aaaaggtgta ccgctgccgg
    10381 gtcaatcact gccagtgaaa atgaaatgcc cgcacacagt aacatcacaa tcaaaaatcc
    10441 cagactcatc cctgccagca cgcgggtact ttgacgaaat ccatgcgacg tagcagagct
    10501 aagggcgaga atattgttcg gtcctggcgt catagcggta atcagggtgt aagtccaaaa
    10561 agcacttaaa agggtcggtg tcacttgtta tctcctctgt tttggtagac agtacccgac
    10621 acggaacatt taaaaaaatg aatgttttta tattctagat tcgatatttt cgataggttt
    10681 ggggttatgg atctgcgccg ttttattacg cttaaaaccg tggtggaaga gggttccttt
    10741 ttgcgagctt cgcaaaaatt gtgctgtaca caatcgacgg tgacttttca tattcagcag
    10801 cttgagcagg agttttcagt ccagttattt gagaaaattg gccgacggat gtgccttacc
    10861 cgcgaaggaa aaaagttgct gccgcacatt tatgaactta cccgagtgat ggatacgctt
    10921 cgtgaggctg ccaaaaagga gtcagatccg gacggggaac tacgcgttgt ttcgggcgaa
    10981 acgttgctct cttaccgaat gccgcaggtg ttgcagcgtt ttcggcaacg cgcaccaaaa
    11041 gtgcgtttat cgttgcagtc gctgaactgt tacgtgatcc gtgatgcgct actgaatgat
    11101 gaggccgatg tcggcgtctt ttatcgcgta gggaatgatg atgccctgaa ccgacgagag
    11161 ttgggtgaac aatcgctggt gctggtggct tcaccacaaa ttgtagatgt cgattttact
    11221 gagccgggaa gacataacgc ctgtagcttt attatcaatg aaccacaatg tgtcttccgg
    11281 cagatatttg agagcacgct gcgtcagcgg cggatcacgg tggaaaacac cattgagctt
    11341 ataagtatcg aaagcatcaa gcgttgtgtc gcggcgaata tcggagtcag ctacctgccg
    11401 cgttttgcgg tggcaaaaga gttggagtgc ggagaattaa ttgaattgcc ttttggcgaa
    11461 cagtcgcaga ccattacggc catgtgcgca caccatgctg gaaaagcggt tagcccggcg
    11521 atgcacactt ttattcagtg tgttgaagag agttttgtgg ctggataaaa ataatgtcgg
    11581 gattaaaccc ggcatttaac ttgtcgacaa agtgcgcttt attcatgccg gatgcggcgt
    11641 gaacgcctta tccggcctac aagatcgtgc aaattcaata tattgcatga gccacgtagg
    11701 cctgataagc gtagcgcatc aggcagtttt gcgtttgcca gcacttttaa taccaggctt
    11761 aaacccggca ttgagattac tcttctgtcg tttgcggtgg cacgcccgtt ccgcttggtt
    11821 tacggcgctt accaatattt ttggtgtcgc gatggcgttt tttcacccgc ggtttctctt
    11881 tctctttagc ttttttcttc tcggcgcgtt ttgccagtac tttcttcgat ggcttgccgg
    11941 tctgcttctc gcttggcgca cgcgttttcg ggcgtaactc atcaataacg cgagctttaa
    12001 tcggctcttc aatgtaacgg ccaaccttgc ccagcagcaa atggtcatgg gcttccacca
    12061 gcgaaattgc ggtgccttta cgaccggcgc gcgcggtacg tccgatacgg tgcaaataag
    12121 tatcgccact gcgcggcata tcgaagttaa agacgtggct gacgtcagga atgtcgatac
    12181 cgcgcgcggc aacatcggtt gcaaccagta cgtttacgcg accttcggtc aaacgcttga
    12241 tcgcttcgtt acgcttgcct tgtaccatct caccttcgag atagcagttg ttgatgcccg
    12301 cttcgcgcag ccagtttgcc agctcatgca cacgttcacg cttacgcaca aacacaattg
    12361 agcgggtcgc ttccggctgt tttaacagat gcaccagcaa tgcggtttta tgttccaggt
    12421 catcggcgcg gtaataccac tgatgaattt ttttgcgctc acgggtggac ggattggcag
    12481 aaacctccac cggatcttcc agcagacgct cggcaaagtc ctgaatcgca tcgccttcca
    12541 gcgttgccga aaagagcagg gtctgtttac gccagcgcgt ttcgccagca atatgttcga
    12601 tatcctgagc gaagcccata tccagcatac ggtctgcttc gtcgaggatc agcgtttcaa
    12661 ccgcgcggca atcgaagttc tcttctttta tgtattgcag cagacgtccg gtcgtggcga
    12721 ccacgatgtc ctgattttcg ctgaacactt ccgcgtggtt catataggct acgccgccgg
    12781 tgatggtggc gatatccaga tgcgtatgtt tcgccagttc gcgggcatga tcggccacct
    12841 gcatcgccag ctcgcgagtt ggggtgagga tcaaaatacg cggcggaccg gatttcttac
    12901 gtgggaaatc gagcaggtgc tgcaacgctg gcagcagata cgccgccgtt ttaccggtgc
    12961 ctgtcggcgc agaaccgagt acatcacggc catcgagcgc aggcggaatg gcggcagcct
    13021 gaatggcggt cgggcgagtg aaacctttat cctggagggc ttccagcagg ctttcgtcga
    13081 gttcaagttc ggaaaaagtc gttacagtca tgttctacct ctgtgtgggg cgctgattat
    13141 agacgttacg gctgcaatct tcatctgttt gtatggatat cgcttctcgg gtattgttct
    13201 cacccggtgc tatcgccgtt tttcctgcaa ggttttattt tcatgtcaca gtctacatcc
    13261 gtgcttcgtc gtaatggatt tacttttaaa cagttttttg ttgctcacga tcgctgtgcg
    13321 atgaaagtgg gaacggatgg cattttgctg ggcgcatggg caccggtggc tggggtgaaa
    13381 cgttgccttg atatcggcgc gggtagcggg ttgctggcac tgatgctggc gcagcgaacc
    13441 agtgacagcg tgataattga tgccgttgag ctggaaagtg aagctgccgc gcaggcgcag
    13501 gaaaatatca accagtcccc gtgggcagag cggattaacg tccatacggc ggatattcag
    13561 cagtggctca cacagcagac agtacgcttc gatttaatca tcagtaaccc accttactat
    13621 cagcagggag tagagtgcgc gacacctcaa cgggaacagg ctcgctatac cactacgctt
    13681 gatcacccat cgctgctgac ctgtgcggcg gagtgcatta ccgaagaggg atttttctgc
    13741 gtggtgctgc cagagcaggt tggtaatggt tttactgaac tggcattaag catgggctgg
    13801 catttacgtt tgcggacgga tgtggcggaa aacgaagcgc gactgccgca tcgggtgctg
    13861 ctggcattct ccccgcaggc gggagaatgc tttagcgatc gcttagtcat tcgtgggcca
    13921 gaccagaact attccgaagc gtatacggcg ctgacccagg ctttttatct gtttatgtaa
    13981 tgattgccgg ggggaaggat cgatggaccg gaatgcgtga gcagttccgg gtaatccagc
    14041 gtaaaatgca ggccacgact ctccttgcgc atcatcgcac agcgaacaat caactcggca
    14101 acctgtacca gattacgcag ctccagcaaa ttattcgaca cgcggaagtg ggcgtaatat
    14161 tcgtctattt cctgttgcaa catattgata cgccgcaggg cgcgctcaag acgttttgtt
    14221 gtgcgcacaa tgccgacgta atcccacata aacagccgta gctcgtgcca gttatgctga
    14281 attactaccc gttcgtcagg gttctcaacg cggctttcat cccacggtgg taacgtactg
    14341 acgccgtggg catcaggcat acgtctggtg atatcttctg ccgccgacca gccatagacc
    14401 agacactcca gcaatgaatt cgaggccatg cggttagcgc cgtgtaagcc ggtataactc
    14461 acctcgccaa tggcatacaa gccctcgacg tccgtacgcc catgatcatc aaccattaca
    14521 ccaccgcagg tatagtgtgc agcaggcaca atcggtaccg gttcttgtgt gagatcaatc
    14581 cccagcccga gcaatttttc ataaatcatc gggaaatgct ggcgaataaa atcggcgggc
    14641 ttatggctga tgtcgaggac catacaatct gcgccgaggc gtttcatttc atggtcaatg
    14701 gcgcgggcga caatatcgcg cggggccagt tcgccgcgct catcaaaatc gggcataaaa
    14761 cgcgtgccat ccgggcgctt gagataagcg ccttcgccgc gcagtgcttc cgttaacagg
    14821 aaattgcgtg cctgtgggtg atacagtgcg gtagggggga actgattaaa ttcgagattg
    14881 gcaacc
//
LOCUS       DAZPLI010000098        14621 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZPLI010000098 DAZPLI010000000
VERSION     DAZPLI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.099"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            73..1608
                     /gene="lsrA"
                     /locus_tag="ACHUU3_002840"
                     /pseudo
     CDS             73..1608
                     /gene="lsrA"
                     /locus_tag="ACHUU3_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            1602..1778
                     /locus_tag="ACHUU3_002841"
                     /pseudo
     CDS             1602..1778
                     /locus_tag="ACHUU3_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(1786..1875)
                     /locus_tag="ACHUU3_002842"
                     /pseudo
     CDS             complement(1786..1875)
                     /locus_tag="ACHUU3_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2082..3530)
                     /gene="uxaB"
                     /locus_tag="ACHUU3_002843"
     CDS             complement(2082..3530)
                     /gene="uxaB"
                     /locus_tag="ACHUU3_002843"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR2082860.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3757..4707)
                     /locus_tag="ACHUU3_002844"
     CDS             complement(3757..4707)
                     /locus_tag="ACHUU3_002844"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR2082861.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4846..5205)
                     /locus_tag="ACHUU3_002845"
     CDS             complement(4846..5205)
                     /locus_tag="ACHUU3_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFR2082862.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(5205..6131)
                     /gene="glsB"
                     /locus_tag="ACHUU3_002846"
     CDS             complement(5205..6131)
                     /gene="glsB"
                     /locus_tag="ACHUU3_002846"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR2082863.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(6195..7583)
                     /gene="sad"
                     /locus_tag="ACHUU3_002847"
     CDS             complement(6195..7583)
                     /gene="sad"
                     /locus_tag="ACHUU3_002847"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFR2082864.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            7684..8565
                     /locus_tag="ACHUU3_002848"
     CDS             7684..8565
                     /locus_tag="ACHUU3_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR2082865.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            8642..9757
                     /gene="yneK"
                     /locus_tag="ACHUU3_002849"
     CDS             8642..9757
                     /gene="yneK"
                     /locus_tag="ACHUU3_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR2082866.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            9907..11097
                     /gene="ydeA"
                     /locus_tag="ACHUU3_002850"
     CDS             9907..11097
                     /gene="ydeA"
                     /locus_tag="ACHUU3_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR2082867.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(11122..11787)
                     /gene="marC"
                     /locus_tag="ACHUU3_002851"
     CDS             complement(11122..11787)
                     /gene="marC"
                     /locus_tag="ACHUU3_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR2082868.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            11999..12433
                     /gene="marR"
                     /locus_tag="ACHUU3_002852"
     CDS             11999..12433
                     /gene="marR"
                     /locus_tag="ACHUU3_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR2082869.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            12453..12836
                     /gene="marA"
                     /locus_tag="ACHUU3_002853"
     CDS             12453..12836
                     /gene="marA"
                     /locus_tag="ACHUU3_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR2082870.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            12868..13086
                     /gene="marB"
                     /locus_tag="ACHUU3_002854"
     CDS             12868..13086
                     /gene="marB"
                     /locus_tag="ACHUU3_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR2082871.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(13143..14582)
                     /locus_tag="ACHUU3_002855"
     CDS             complement(13143..14582)
                     /locus_tag="ACHUU3_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR2082872.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         3786 a         3745 c         3392 g         3698 t
ORIGIN      
        1 taaggttatg aacaaattaa aagcagaaat acatttgttc aaaactcacc tgcaaaactg
       61 aacgggggaa atatgcaaac gagtgatacc cgcgcgttac cgctactttg cgcccgctcg
      121 gtttataaac agtattcagg ggtcaatgtc ctgaaaggca tcgattttac gttgcatcaa
      181 ggggaggtcc acgccctgct cggcggcaat ggtgccggta aatcgacgtt aatgaagatt
      241 attgccggta ttacccctgc tgatagcggt acgctggaga ttgggggcaa caactacgtc
      301 agattaacgc cagttcatgc tcatcagctg ggtatttatc tcgttcccca ggaaccgctg
      361 cttttcccaa gcctgtcgat aaaagaaaac atcctgtttg ggctggcaaa aaaacagctc
      421 tccatgcaga aaatgaagaa cttgctggcg gcgctgggct gctagtttga tctgcatagt
      481 ctggcaggat cgctggatgt cgccgatcgc caaatggtgg aaatcctccg cggcctgatg
      541 cgcgactcgc ggattctgat cctcgatgaa cctaccgcct cgcttacccc tgcggaaacc
      601 gaacgcttgt ttcgtcgctt gcaagagctg cttgctactg gcgtgggtat tgtttttatc
      661 tcgcataagc tgccggaaat tcgccagatt gccgatcgaa ttagcgtgat gcgcgacgga
      721 accatcgcct taagcggcaa aaccagcgaa ctgtctaccg atgacattat tcaggccatc
      781 acgccagtgg tacgggaaaa atcgctctct gccagccaaa aattatggct ggagttacct
      841 ggcaaccgcc cacaacatgc cgccggaacg tcggtgctga cactagaaaa tctgaccggc
      901 gaaggtttca ggaatgtcag cctgacgctc aatgccggag aaattctggg tctggccggg
      961 ctggtggggg ccggacgcac agaactggcc gagacgctct atggtctgcg tactttgcgt
     1021 ggcggacgca ttatgctgaa tggtaaagag atcaataaat tatccactgg agaacgttta
     1081 ctgcgcggtc tggtttatct gccggaagat cgccagtcat ccggactgaa tctcgatgct
     1141 tcgctggcct ggaacgtctg cgcccttact cataaccttc gtggattctg ggcgaaaacc
     1201 gcgaaagata atgccaccct ggaacgttat cgtcgggcgc tgaatattaa atttaaccaa
     1261 ccggaacaag ctgcacggac attatccggt ggcaaccagc aaaaaatcct gattgccaaa
     1321 tgcctggaag cttcgccgca agcattgatt gtcgatgagc cgacgcgcgg cgtggatgtc
     1381 tcggcccgta atgatatcta ccagctgttg cgcagcatcg ccgcacagaa tgtggcagtg
     1441 ctgcttatct cctctgatct ggaagagatc gaactgatgg cagatcgtgt gtatgtgatg
     1501 catcagggcg aaattaccca ctctgcactg accgggcgcg atattaatgt cgagactatt
     1561 atgcgcgttg ccttcggcga tagtcagcgt caggaggcgt catgctgaag tttattcaga
     1621 acaaccgtga aatcacggca ctgctggcgg tggtgctgct gtttgtatta cccggttttc
     1681 tcgaccgcca gtatttaagt gtgcaaacgc tgaccatggt ttatagcagc gcgcaaatcc
     1741 tgatcctgct ggcaatgggc gcgacgctgg taatgcttat gcgcaaagta gtaagcgcga
     1801 tgatatcttg gataatactg agccgtgcca gacaaaaata cggcgcagcc agtgttgttg
     1861 tggacgaaca atcatggtta tcttcaggcg tgaaaaagtc gccctattaa acgtgattac
     1921 gatcacattc tcaagacgct acttacaaat tacctacaag cttacaacat taacaggcgc
     1981 tgcatgtggc atctgttaat gataaaggca cataggatgt tgtaactaat atagtgaaat
     2041 aagaaacccg gtcaaaaccg ggttcagaag taacggtgtt attagcacaa cggacgtaca
     2101 gcttcgcgca tccctttttc aagaatcgca tccaggtcat tagcaacctg ctcgaccaga
     2161 ccaggcactt gcgtcaggtc ctgctcccag tggtcttttt ctgccaacac aatcgctacc
     2221 agttcctggg taccgataac atggtcacga tgctggctcc agagttgctg ataacgttcc
     2281 agccagtgtg catcgtcctg taccggatat gtttctccgt tacgctcacc acgatagaac
     2341 gcaatcaatg ccgctaatgc gaaagtaagg cgcgccggaa gtgtgccgtt tgccttctgc
     2401 cctgccagca gctgcggcag gatgcgggtg cggaacttgg tcataccgtt gagcgcgata
     2461 gacagcagct gatgcttata cgggttacgg aaacgcccgg tgactgcact ggcgaaagat
     2521 tccagttcat cacgaggcaa atccagtacc ggaataattt cttcgtagat ggctttttca
     2581 acgaatgcgc aaatttcagc atcgttcatc gcttcaccta cggtatccag ccctgcctgg
     2641 aacgccaccg gcaccagcgc ggtgtgcgca ccgttgagga ttgccacttt gcgttcttta
     2701 tacggtttaa tatcgtcaac aatcagcacg ttgagcggat atttgtccag gcgcagctca
     2761 gtcgctaagg atttcggccc ctgaatcaca aacaggtaaa agtgttcagc ggtgtcgagg
     2821 aaaccatcgt gataacccag ttcttcttcc agtttagcca cttcatcgcg cggataaccg
     2881 gtaacgatac ggtccaccag cgtagaacag aagctgttag cctgatccag ccattgaata
     2941 aatgcttctg gcagtgccca ctcttgcgca tagcgcagca ccagttcacg caacgcgtcg
     3001 ccattgtagt cgatcaactc acacgggatg atgatccaac ctttatccag cgcaccgttg
     3061 aaatggctaa agcgttcgaa cagcagacgg gtcagttttg ccggatagct tatcgctggc
     3121 gcgtcatcga atttatcgcc cgcatggtag ctgatccccg cttcggtggt gttagagaaa
     3181 acaaagcgca tttccgggtt gtgcgccagt ttcaggaatt catcgtattc actgtagacg
     3241 ctgatttcac gattaaccga gcgaatcaga cgcgcgtcgc tgaccgcttc ccccttctca
     3301 ttcaggccac ggataatggt ggtatacaga ccatcctgcg tgctcagtga cggcgggaat
     3361 gaagtttcaa tcggacgaac aacgaccacg ccagaattca gatcggtgtg ctcattcagg
     3421 agatcgattt gccagtcaac aaaggcgcgc aggaagttac cttcaccaaa ctgaatgata
     3481 cgttctggat actgtgcacc gggaaaatcg cgacgattta gtgttttcac aatgggttcc
     3541 cttctgatta gtcatacaac ctgtttgaat tggtacgaca ggttagcaaa ctttaatacg
     3601 ccgaacccct gttttgatca actcctgatg attaatgagc agttttatga gaaaagtgtg
     3661 gcgcggatca tggtttaatc gaggaaatac gccttttcct ggatcataaa gtggtagaac
     3721 acattgcatt caaatcgcgc ggaatgaata aagatgtcag acaacttcct caccgtaacg
     3781 catagtgctg gtacggttgc gcccatcttt cttcgagcga tacagacacg tatcggcttc
     3841 gaccatcaat ttattaaaat catcggtcag cgttcggtac gatgcgcgac cactaccgac
     3901 gccaatactt accgtgagat aaagcgtttt ttgttgccag gtgaatggtt gcagttcaac
     3961 gcctttacgg attttttccg ccattagcag accatcgaca ggattcaccg acggcactgc
     4021 aacagcaaat tcttcgccgc ccatgcgcgc caccagcccc ttatcaccga caatcttctg
     4081 aatatgccgg gcaaacacgc ttaacacttt atcgccacat tcatgtccat agttatcgtt
     4141 aatgcttttg aaatagtcga tatcaagcag catgaccgtc agaggcacga tcaacacgcc
     4201 cgttgagaat tgctcactga accagtccgc aagcaaaggc cagaaatcca gactatcaat
     4261 actgaccgaa ccaatcgccc cgacaatagc gcataataat gcggcaatca gacagtaatt
     4321 aaatagccgt aaggcactta ctggctcata cttatttttc cccagacgct tatcccgcac
     4381 gaccagtaag gcgacggtaa caataaacat catattggag aaattgataa ccagtgaaac
     4441 cagtccccat tcggtggtta tggcatcata aaccagcatc gccacataac tgatcgcata
     4501 gtaatgcagt cgattaagcc agacatagcg ggcaaacccc cccgccatta cgccattcag
     4561 cggccagaaa agagaaagtt cgtgaaccag ccgtaactct gcaccaataa aatagaacag
     4621 cgtggttagc gcaaaaatag cgatgctatt acgtagcaga tgaccttcct ggaacaaacg
     4681 gaaagttgaa atggggtgta catgcattaa ttatctgtcc atacaattgc gggtggcaat
     4741 tatctcaacg taataagtaa attctcataa aggatagcat gttcgctaac agcggcggca
     4801 aataaaggta ccctgggaat gctattttat cgttttccgc aattctcatg gctgattcat
     4861 ctgtacaacc agccagtgat agattaccgc cacgatataa cgttgttgtt cttcgcttaa
     4921 cgaaacaccc tgcggaatgt tatgccactt agccagacaa ccgcgacagc aggtcgcggt
     4981 ggcgtgctga gcgataaaca ccggatgccc gcgcatgggg gtttgcttac cgtcattagc
     5041 cggtaatgcc ggagcaagac gtttagcgac aaaatccgcc gcatgttgtt cgatgactgg
     5101 cgcgcctttc tccaggcaat actgacgctc tttcatgccc agacgaaagc gagagcgaaa
     5161 ttttgaacgg gataaacgcg cgaagagtgg atcgagagac tgcattaata aaccgaacgc
     5221 cctaactgtt tcgtcaattg ttcaaggacg gcgatacccg caagcgagtt acctgcatca
     5281 tccagttccg gactccagac tgcgatggcc atttcatgcg gaacaatcgc cacaataccg
     5341 ccaccaacgc cagatttcgc cggtagtccc acccgccagg caaactcccc cgcgttctgg
     5401 tacataccac tggtcgccat cagcgcgtta atttgccgcg cctgcattgg cgtcactaca
     5461 ggttcatcaa tatgaatagc tttcccctga ttagccagaa agacaaacgt ccgagccagc
     5521 tctacacagc tcattttcag agcgcagtaa tgaaagtagt tttgcagaac ggttgtcacg
     5581 tcatgatgga aattgccaaa cgacttcatc agccaggcga tagccgcatt tcgcgcggaa
     5641 tgttcaaatt cggaacgcgc taccaccgta tcgtatgaaa tatcagacac accgcttaag
     5701 ccgcgcacga cttccagcat acgttgccgt ggtgcgctta atcgcccttg caacatatcg
     5761 cagaccacca gcgcaccagc attaataaac ggattacgcg gaataccctg ttccatttcc
     5821 agttgcacta aagaattgaa cggtgatcca gacggatctt tgccgacgcg ttgccagatt
     5881 tcctcttcgg agtaatgacg catggcgaca acgagactca gcactttgga aatagactga
     5941 atggaaaaac gttcttgcgc gtctccggcc tgaaaaagct gtccgtcaac ggtacagata
     6001 gcaatgccca atcgggaacc gtctactgta gccagcgccg gaatataatc cgcgacttta
     6061 ccctgaccaa tgagcggccg cacttgccgc aagatgtttt ctaaaattgc attatccatg
     6121 gcgactgcca ctttctactc ctggaccgca ggtctgaaaa gacctgcgag tatatcagag
     6181 ctgaatatgt cgcgtcagat ccggtctttc cacaccgtct ggatattaca gaattcgtgt
     6241 aagccgaaat gggaaagctc acgaccaaag ccactctttt tcacgccacc aaaggccact
     6301 cgcgcgtcgc tggcacaata accattgata aacaccccac cgcattccag acgtgccgcc
     6361 atctgtctgg cctgtgtttc gtcggtggta aaaatggtcg ctgaaaggcc gaactcacta
     6421 tcattagcca gttccagcgc atgttctgca tctttcgcaa cggtgattgc cgcaacgggg
     6481 ccaaacattt cttcccgaaa cgcggtcatt tctggggtaa cattcgccag aaccgttggc
     6541 ggatagtagt tacctgcccc agccatcttt tccccgccca gtaacaaacg cgcaccctgc
     6601 gccagggttt tctctacctg atgatgcagc tcatcacgta aatcaaaacg agccattggt
     6661 ccgagagtgt tctcttcgtc acggggatcg cccattttca aggctgccgc agctgccaca
     6721 aaacgttcgg taaatgcaga agcaattccc tcttcgataa taaagcgttt tgccgctgca
     6781 catacctgtc cggtattctg ataacgtccg gctaccgccg ctttaaccgc cagttccaga
     6841 tcggcatcgt taagcacaat aaacggatcc gaaccgccca gttccagtac gcattttttc
     6901 agtgccgctc cagcctgtgc gccaatagcc gctccagcac gaacacttcc ggtcaccgtg
     6961 acagcagcaa tgcgcgagtc tttaatcatc tgactgacac cgtcgttgtc ggcattcagc
     7021 cagccatata cgccttgcgg gattcccgca tctttaaaca cctgggcaat gagctgagca
     7081 catcccatca cattcggcgc atgtttaagt aagtagccgt tacctgcaag aatgatggga
     7141 acagcgccac gcatcacctg ccataacgga aaattccacg gcataatcgc cagaatcgtc
     7201 cccaacggtc gatactcaat aaccgcctgc tgattttcca ccagtgtagg ttccgccttc
     7261 agcattgccg gaccatgttc tgcataccag tcacacaaat tcgccgattt cgccacttca
     7321 gcgcgcgcct ggttgattgg tttgcccatt tcgcgggtga tcatttgcgc catttcttcg
     7381 ctacgggcgc gcagagcctt accgatatca cgcatttttt cagcacgata atctatattt
     7441 gtctcgcgcc agtcgcgaaa gcctgctgcc gccagctcaa gtgcgttttc gatatcgtta
     7501 gcgccagccc acggcagcac agaaagttgt tcacccgtgg caggatttat tgaaattgca
     7561 tgagttgccg gagtaatggt catcggggta tctcctttat gagtcatggt atgaagatac
     7621 gcagatttac tcttgcttta aaatgaataa tattaagcca cttattcacg aatcgagaat
     7681 gttatggatc tgacccaact ggagatgttc aacgcggttg ccgaagctgg cagcattacc
     7741 caggctgcag caaaagtgca tcgcgtaccg tcgaatctca ctacccgtct acgccagctg
     7801 gaaacagaac tgggggtaga tctgtttatt cgcgagaatc agcgtttacg tctctctcct
     7861 gccgggcata actttttacg ctatagccaa caaattctcg cgttagtgga tgaagcgcgg
     7921 agcgttgtcg ctggcgatga gccgcaaggt ttattttctc ttggttcgct ggaaagcacc
     7981 gcagcagtgc gcattccggc cacgctggcg gaattcaacc atcgttatcc caaaattcag
     8041 ttttcacttt ccaccggccc ttccggcact atgctggaag gagtgctgga gggaaaactg
     8101 aatgcggcat ttattgatgg gcccattaac catactgcca ttgacgggat accggtatac
     8161 cgcgaggagc tgatgatcgt cacgccacaa ggtcatgcgc cagtaatccg cgccagtcag
     8221 gttaacggca gtaacattta tgccttccgc accaattgtt cgtatcgtcg ccacttcgag
     8281 agctggtttc atgctgacgg tgccgctccg ggaactattc acgagatgga gtcgtatcac
     8341 gggatgttgg cctgtgtggt cgcaggagca ggcattgcgc ttattccacg ctctatgctg
     8401 gaaagtatgc cggggcatca ccaggttgaa gcgtggccgt tagctgagca atggcgttgg
     8461 ttaacaacct ggctggtctg gcgtcgcggt gcgaaaacgc gtccgcttga ggcatttatt
     8521 caactactgg atgtgtctga ctcggcaaaa cagagttacc aatgaactat tttgatagtt
     8581 cttgcggcta atatgctcta tatagtgatg ttccgatgac ttatgactat atggggcaaa
     8641 tatggttacg ccagtaagca tcagcaatta catatctctt cctgatgatt ttcccgttcg
     8701 taatattgca ccacaggtaa aagaagtttt aaaggacttt attgatgcgc taagtacaat
     8761 aatatgtgat gaagagtggc gcacgtcttt aaacatcaac tcagccacaa aaaagatatt
     8821 taataacctt gataacttat catatattca gaggacttct ttccggggta acgacacgct
     8881 atacaatgaa aaggttcagt ttaaactcac ttatcccgtc aagaatggaa gacacaaaga
     8941 aaatattgaa tttcaggtag taataaattt aagccctatt tatttagata attttcgcca
     9001 tgatggagaa attaatattt tttgcgctcc caacccaaag cctgtcacta tggggcgcgt
     9061 atttcagacc ggcgtcgagc gtgtgctctt tctttttctg aatgatttta ttgaacaatt
     9121 tccaatgttc aaccctggcg ttccgatcaa aatagcgcat acaccacata ttgaacccct
     9181 gcctccggat caccataccg ctgcagatta cttacgccag tttgatttgc ttgtcctgaa
     9241 ttttatctct cgcggtaatt ttgtcatact cccccgatta tggaataact ctgaggttca
     9301 cagatggttc gtcaataaag atcctaactt gatcaccgcc attctcgaca taacggacag
     9361 cgaattaaaa gaggatttat tgcaaagcct gatggattca ttaggttcta acaaacatgt
     9421 actacccgaa gtctgcatct gctttttatt ccttttagca gaacaagaat ctcctcattt
     9481 tcaggacttg tttttatttt tcgccaatat gttattgcac tatcaccaat ttatgaatcc
     9541 caatgaaagt gatttgaatg acgtgttgat gccagcatca ttaagtgatg ataaaattat
     9601 caagcatatg gcgcgcagga ccctcgaact gtttgtaaaa aatgaaacac cgccaaaagt
     9661 tactcacgaa gacctggtga aaaacaggcc tcgctcccct gtcagaccac ctatacccgc
     9721 aacctccaaa acgccagacc tccctgaacg tcattaaacc gtgatgttac cgactctctg
     9781 acgcggaaca gaatcagcgt cagagaaacg gaaaacgcga tccagatcac aaatacattg
     9841 tattcacatc attaaccgtt ttaagatcat ttcatcactt tttcgcaact cacccgataa
     9901 tctgttatga caacaaacac tgtttcccgc aaagtggcgt ggctacgggt cgttacgctg
     9961 gcagtcgccg ccttcatctt caacaccacc gaatttgtcc ctgttggcct gctctctgac
    10021 attgcgcaaa gttttcacat gcaaaccgct caggtcggca tcatgttgac catttacgca
    10081 tgggtagtag cgctaatgtc attgcctttt atgttaatga ccagtcaggt tgaacggcgc
    10141 aaattactga tctgcctgtt tgtggtgttt attgccagcc acgtactgtc gtttttatcg
    10201 tggagcttta ccgttctggt gatcagtcgc attggtgtgg cttttgcaca tgcgattttc
    10261 tggtcgatta cggcgtctct ggcgatccgt atggctccgg ccgggaagcg ggcacaggcc
    10321 ttgagtttaa ttgccaccgg tacagcactg gcgatggtct taggtttacc tctcgggcgc
    10381 attgtggggc agtatttcgg ttggcgaatg accttcttcg cgattggtat tggggcgctt
    10441 atcacccttt tgtgcctgat taagttactt cccttactgc ccagtgagca ttccggttca
    10501 ttgaaaagcc tcccgctatt gttccgccgc ccggcattga tgagcattta tttgttaact
    10561 gtggtggttg tcaccgccca ttacacggca tacagctata tcgagccttt tgtgcaaaac
    10621 attgcgggat tcagcgccaa ctttgccacg gcattactgt tattactcgg tggtgcgggc
    10681 attattggca gcgtgatttt cggtaaactg ggtaatcagt atgcgtctgc gttggtgagt
    10741 acggcgattg cgctgttgct ggtgtgtctg gcactgctgc tacctgcggc gaacagtgaa
    10801 atacacctcg gggtgctgag tattttctgg gggatcgcga tgatgatcat cgggcttggt
    10861 atgcaggtta aagtgctggc gctggcacca gatgctaccg acgtcgcgat ggcgctattc
    10921 tccggcatat ttaatattgg aatcggggcg ggtgcgttgg taggtaatca ggtgagtttg
    10981 cactggtcaa tgtcgatgat tggttatgtg ggcgcggtgc ctgcttttgc cgcgttaata
    11041 tggtcaatca ttatatttcg ccgctggcca gtgacactcg aagaacagac gcaatagttg
    11101 aaaggcccat tcgggccttt tttaatggta cgttttaatg atttccagga tgccgttaat
    11161 aataaactgc acacccatac ataccagcag gaatcccatc agacgggaga tcgcttcaat
    11221 gccacccttg cccaccagcc gcataattgc gccggagctg cgtaggcttc cccacaaaat
    11281 aaccgccacc aggaaaaaga tcagcggcgg cgcaaccatc agtacccaat cagcgaaggt
    11341 tgaactctga cgcactgtgg acgccgagct aataatcatc gctatggttc ccggaccagc
    11401 ggtacttggc attgctagcg gcacaaaggc aatattggca ctgggttcat cttccagctc
    11461 ttccgacttg cttttcgcct ccggtgaatc aatcgctttc tgttgcggaa agagcatccg
    11521 aaaaccgata aacgcgacga ttaagccgcc tgcaattcgc agaccgggaa tcgaaatgcc
    11581 aaatgtatcc atcaccagtt gcccggcgta atacgccacc atcatgatgg caaatacgta
    11641 caccgaggcc atcaacgact gacgattacg ttcggcactg ttcatgttgc ctgccaggcc
    11701 aagaaatagc gcgacagttg ttaatgggtt agctaacggc agcaacacca ccagccccag
    11761 gccaattgct ttaaacaaat ctaacattgg tggttgttat cctgtgtatc tgggttatca
    11821 gcgaaaagta taaggggtaa acaaggataa agtgtcactc tttagctaac cttgcatcgc
    11881 attgaccaaa acttgaaccg atttagcaaa acgtggcatc ggtcaattca ttcatttgac
    11941 ttatacttgc ctgggcaata ttatcctctg caactaatta cttgccaggg caactaatgt
    12001 gaaaagtacc agcgatctgt tcaatgaaat tattccattg ggtcgcttaa tccatatggt
    12061 taatcagaag aaagatcgtc tgcttaacga gtatctgtct ccgctggata ttaccgcggc
    12121 acagtttaag gtgctctgct ctatccgctg cgcggcgtgt attactccgg ttgaactgaa
    12181 aaaagtgttg tcggtcgacc tgggagcact gacccgtatg ctggatcgcc tggtctgtaa
    12241 aggctgggtt gaaaggttgc cgaacccgaa tgataagcgc ggcgtactgg taaaacttac
    12301 caccagcggc gcggcaatat gcgaacaatg ccatcaatta gttggccagg acctgcatca
    12361 agaattaaca aaaaacctga cggcggacga agttgcaaca cttgagcatt tgcttaagaa
    12421 agtcctgccg taaacaaaaa agaggtatga cgatgtccag acgcaatact gacgctatta
    12481 ccattcatag cattttggac tggatcgagg acaacctgga atcgccactg tcactggaga
    12541 aagtgtcaga gcgttcgggt tactccaaat ggcacctgca acggatgttt aaaaaagaaa
    12601 ccggtcattc attaggccaa tacatccgca gccgtaagat gacggaaatc gcgcaaaagc
    12661 tgaaggaaag taacgagccg atactctatc tggcagaacg atatggcttc gagtcgcaac
    12721 aaactctgac ccgaaccttc aaaaattact ttgatgttcc gccgcataaa taccggatga
    12781 ccaatatgca gggcgaatcg cgctttttac atccattaaa tcattacaat agctagttga
    12841 taacgtgaca acgtcactga ggcaatcatg aaaccacttt catccgcaat agcagctgcg
    12901 cttattctct tttccgcgca gggcgttgcg gaacaaacca cgcagccagt tgttacttct
    12961 tgtgccaatg tcgtggttgt tcccccatcg caggaacaac caccgtttga tttaaatcac
    13021 atgggtactg gcagtgataa gtcggatgcg ctcggcgtgc cctattataa tcaacacgct
    13081 atgtagttat tgtcaggctc tgatcacaga gcctgacatc tccgtaatat cttcctcaag
    13141 tctcaaatat tttcgccatt gttggcgatg agatcccgat accagtaaaa cgacgcttta
    13201 cgtatccgtt ccagcgttcc cttcccttcg ttgtctttat cgacataaat cattccgtag
    13261 cgctttttca tttcccctgt tccggcagaa accaggtcaa tgcagcccca cggggtgtag
    13321 ccaattaagt caacaccatc ttcaactacg gcttttttca tttcccgaat atgggaagca
    13381 aagtaatcaa tgcgatagtg atcgttcacc gtgccgtcag cttgtctctg gtcaaccgca
    13441 ccaaatccat tttcgacaat aaacagcggc aactggaaat gatcccagaa ccagttgagt
    13501 gaataacgta gccctgccgg atcaatttgc catccccatt cggatgtatc gatataaggg
    13561 ttgctgacca aatctcgtgg ttcaacataa tccagttgcg ggttatcggg agaaaattgt
    13621 gtcgtaaaag acatgtagta gctaaaaccg ataaagtcga cacatccact ggcaagaatc
    13681 gcgttatctt ctggtgtgat atcgaggttg aatcctttcc tggcaaagta attcagcata
    13741 tgttgcggat aatatccacg agcatgaaca tcagtaaacc agtaacgacg atgcatcgct
    13801 ttcgtggcca tcatcatatc gttgggtgca cacgtcagag gatagatggg gcacatggcg
    13861 atcatacagc caatgttaaa ttcaggattg atcgattttc cagtctgtac cgccagggca
    13921 ctggcaacta actcgtaatg taccgcctgg tacattattt gttcgcgctc attgatatct
    13981 tcctctggcg aatacaagat accggaatta gtaaatggac acaggctttc gctgaaattc
    14041 acctgattat tgatttcgtt aaacgtcatc cagtacttta ctttttcttt atagcgcgtg
    14101 aagaccgttg atgcgaagcg gataaaaaag tcgatcagct tacggtttcg ccagccacca
    14161 tattttgtca ccagatgata aggcatctca aaatgcgaaa gcgtcaccac aggttccatt
    14221 ccttgcttca ggcattcatc gaacagatca tcataaaatt gtaaaccctc ttcattcggc
    14281 tcctgttcgt caccttgcgg aaagattcgt gtccaggcaa tggaagttcg aaagcatttg
    14341 aatcccatct cggcaaataa ctgaatatct gttttatagc gatgataaaa atcaattgct
    14401 tcatgattgg gataattaag cccgtcgata acgccttctg tcacttcacg cggcaccccg
    14461 tgagcgccag cagtcatcac atcagcgata ctgatgcctt ttcctccttc attccagcca
    14521 ccttccaact gatgcgcggc taccgcgcca ccccataaaa aacctttttt aaatcctgac
    14581 atacacaact ccttaactaa aatagattac caccagactt c
//
LOCUS       DAZPLI010000099        14368 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZPLI010000099 DAZPLI010000000
VERSION     DAZPLI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.100"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..696)
                     /locus_tag="ACHUU3_002856"
     CDS             complement(<1..696)
                     /locus_tag="ACHUU3_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR2082873.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            952..1107
                     /gene="rseD"
                     /locus_tag="ACHUU3_002857"
     CDS             952..1107
                     /gene="rseD"
                     /locus_tag="ACHUU3_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR2082874.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1104..1679
                     /gene="rpoE"
                     /locus_tag="ACHUU3_002858"
     CDS             1104..1679
                     /gene="rpoE"
                     /locus_tag="ACHUU3_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR2082875.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1712..2362
                     /gene="rseA"
                     /locus_tag="ACHUU3_002859"
     CDS             1712..2362
                     /gene="rseA"
                     /locus_tag="ACHUU3_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR2082876.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2362..3318
                     /gene="rseB"
                     /locus_tag="ACHUU3_002860"
     CDS             2362..3318
                     /gene="rseB"
                     /locus_tag="ACHUU3_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR2082877.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3315..3794
                     /gene="rseC"
                     /locus_tag="ACHUU3_002861"
     CDS             3315..3794
                     /gene="rseC"
                     /locus_tag="ACHUU3_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR2082878.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3992..5791
                     /gene="lepA"
                     /locus_tag="ACHUU3_002862"
     CDS             3992..5791
                     /gene="lepA"
                     /locus_tag="ACHUU3_002862"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR2082879.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5807..6781
                     /gene="lepB"
                     /locus_tag="ACHUU3_002863"
     CDS             5807..6781
                     /gene="lepB"
                     /locus_tag="ACHUU3_002863"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR2082880.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7054..7734
                     /gene="rnc"
                     /locus_tag="ACHUU3_002864"
     CDS             7054..7734
                     /gene="rnc"
                     /locus_tag="ACHUU3_002864"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR2082881.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7731..8636
                     /gene="era"
                     /locus_tag="ACHUU3_002865"
     CDS             7731..8636
                     /gene="era"
                     /locus_tag="ACHUU3_002865"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR2082882.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8648..9376
                     /gene="recO"
                     /locus_tag="ACHUU3_002866"
     CDS             8648..9376
                     /gene="recO"
                     /locus_tag="ACHUU3_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR2082883.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9388..10119
                     /gene="pdxJ"
                     /locus_tag="ACHUU3_002867"
     CDS             9388..10119
                     /gene="pdxJ"
                     /locus_tag="ACHUU3_002867"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR2082884.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10119..10499
                     /gene="acpS"
                     /locus_tag="ACHUU3_002868"
     CDS             10119..10499
                     /gene="acpS"
                     /locus_tag="ACHUU3_002868"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR2082885.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10721..10801
                     /gene="shoB"
                     /locus_tag="ACHUU3_002869"
     CDS             10721..10801
                     /gene="shoB"
                     /locus_tag="ACHUU3_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR2082886.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="ACHUU3_002870"
     CDS             complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="ACHUU3_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR2082887.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            11470..12672
                     /locus_tag="ACHUU3_002871"
     CDS             11470..12672
                     /locus_tag="ACHUU3_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR2082888.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSSRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVYEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQLRDPMQPFSKRSLDKLVKTYSPDGAVKGIKHDGTVKGKDGAFNTMCRKFLKSN
                     VIALIDRN"
     gene            complement(12845..14032)
                     /locus_tag="ACHUU3_002872"
     CDS             complement(12845..14032)
                     /locus_tag="ACHUU3_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR2082889.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(14032..>14368)
                     /locus_tag="ACHUU3_002873"
     CDS             complement(14032..>14368)
                     /locus_tag="ACHUU3_002873"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2082890.1"
                     /translation="KPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCAD
                     LNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVH
                     WPDGVAPPF"
BASE COUNT         3622 a         3606 c         3706 g         3434 t
ORIGIN      
        1 gcccgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta
       61 ctgatacacc ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc
      121 caccgtttct ttatttcggt tccataccca cgcgcctaca acccgtcgcg tgcccggcag
      181 gccaattttg tcagagacaa tcagatcaac tgcgttgctg cgctccagca cacgaatatt
      241 cggatggttc tgcgccttgc tcaccagcgt ggattgtact tctctaccgg tggcgtcggc
      301 ggcatgaaga atacgacggt gactatgtcc accttcacgg gtcagatggt agctttcttc
      361 gccattcggt tgaacgtggg tatcaaacaa aaccccctgg tcaattaacc attgcacaca
      421 ggaacgagca ttgctggcga caaattcaac tgcatggcga tcgcaaatac cagccccggc
      481 aatcaatgtg tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat
      541 accgccctgg gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac
      601 ctgatgctgg tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc taccaataat
      661 caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca
      721 ttgtttggtc agcatagcat catgttgtgc gaataaacac ctgctatttt aatatttgtt
      781 acagtggcta aacacgttga cgcagggcgg cgagaaaaag agaagttact ggctggtgga
      841 ggattaggtg gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt
      901 tctacacaga taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt
      961 ctacagcatg acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta
     1021 accctttgct tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt
     1081 ggtttgggga gactttacct cggatgagcg agcagttaac ggaccaggtc ctggttgaac
     1141 gggtccagaa gggagatcag aaagccttta acttactggt agtgcgctat cagcataaag
     1201 tggcgagtct ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag
     1261 cttttattaa agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat
     1321 ggctgtatcg gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc
     1381 caccttccag tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga
     1441 aagaaatttc gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc
     1501 gaactattga gtccctcccg gaagatttgc gcatggcaat aaccttgcgg gagctggatg
     1561 gcctgagcta tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac
     1621 gtatcttccg agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac
     1681 gatagcggga tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat
     1741 ggatggcgaa acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca
     1801 gaaaacctgg gaaagctatc acttaatccg tgattcaatg cggggtgata ctcccgaggt
     1861 gctccatttc gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca
     1921 accggcgaca ttgatcccgg aagcccagcc tgcgccgcat caatggcaca aaatgccatt
     1981 ctggcagaaa gtacgtccgt gggcggcaca gcttacccaa atgggcgtag cagcatgcgt
     2041 atcgcttgca gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca
     2101 gcccgaaacg ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct
     2161 gggagtacct tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg
     2221 tcgtcgcatt aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca
     2281 gcttcagttt gagcaggcgc aaacccagca agccgctgta caggtgccag gacttcaaac
     2341 tttaggaacg caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt
     2401 agcctgttat tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag
     2461 atgaacctgg ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag
     2521 ggtgttgagt ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg
     2581 ttgcaaatgg atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt
     2641 gaaccgggac ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctgccatcg
     2701 cttatctata ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc
     2761 acgcgtattg ctgatcgtct ttgcgaagtc attcgcgtgg ttgcccgaga tggtacacgc
     2821 tacagctaca tcgtgtggat ggacactgaa tcgaaattac cgatgagggt tgatctcctt
     2881 gatcgcgatg gtgaaacgct ggaacaattt cgcgtgattt cttttaacgt caatcaggat
     2941 atcagcagca gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct
     3001 gtaggtgaaa aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa
     3061 gtttccagta gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc
     3121 tattccgacg gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc
     3181 gatcagatgt tgcgcaccgg acgcagaacc gtcagtacaa gcgtacgtga taacgccgaa
     3241 atcaccattg tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag
     3301 ttcggggcag cgcaatgatc aaagagtggg ctaccgtcgt ctcctggcaa aacgggcagg
     3361 cgctggtcag ttgtgatgtt aaagcctcat gcagcagctg cgcttcacgc gccggttgcg
     3421 gtagccgcgt gttgaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg
     3481 atgaaccgtt agtgccgggg caaaaagtgg aattggggat cgccgaaggc agcctgctta
     3541 gctccgcatt actggtttat atgtcgccgc tggtgggatt attccttatc gcttcgctat
     3601 ttcagctact ctttgcttct gacgttgcag cattatgtgg tgcgatcctt ggcggtattg
     3661 gcgggtttct gattgcgcgc ggttactcgc gtaagtttgc tgcccgggca gaatggcaac
     3721 cgatcatatt aagcgtggca ttgccgcctg accttgtgcg atttgaaaca tcttcggaag
     3781 acgcgagcca gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt
     3841 ccagatacca caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat
     3901 gcggcgtttc tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta
     3961 accgggcatt aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat
     4021 agctcacatt gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg
     4081 cctgtctgac cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg
     4141 tggcattacc atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac
     4201 ctatcagctt aactttatcg acaccccggg ccacgtagac ttctcctatg aagtttcccg
     4261 ttcgctggct gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtggaagc
     4321 gcaaaccctg gcaaactgct acaccgccat ggaaatggat ctcgaagtgg tgccagtact
     4381 gaacaagatt gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat
     4441 cgtcggcatc gacgccactg acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtgca
     4501 ggacgttctc gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggccc
     4561 gttgcaggca ctaattatcg actcatggtt cgacaactac ctgggcgttg tttcacttat
     4621 ccgtattaaa aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca
     4681 gacctataac gccgaccgtc tgggcatctt cacgccgaaa caggttgacc gcactgaact
     4741 gaaatgtggc gaagtaggct ggctggtatg tgcgattaaa gatatccacg gcgctccagt
     4801 cggcgatacc ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa
     4861 agtcaaaccg caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt
     4921 ccgtgacgcg ctgggtaaac tcagcctgaa tgatgcctca ctgttctatg agccggaaag
     4981 ctccagcgcg ctgggctttg gtttccgctg cggcttcctt ggcctgctgc acatggagat
     5041 catccaggaa cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt
     5101 agtgtatgaa gttgaaacca cgtcaaggga agttatctac gtcgacagcc catccaagct
     5161 gcctgcggta aataacattt acgaactgcg cgagccgatt gcagagtgtc acatgctgct
     5221 gccgcaggca tatctcggca acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac
     5281 caatatggtt taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt
     5341 ggtgctcgac ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta
     5401 caacttcaaa cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga
     5461 acgtgttgat gcgttggcgc tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga
     5521 gttggtggag aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc
     5581 agcgattggt acgcacatta ttgcgcgatc caccgtgaaa cagctgcgta aaaacgtact
     5641 ggctaaatgt tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga
     5701 aggtaagaaa cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc
     5761 cattctgcac gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt
     5821 tgccctgatt ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt
     5881 ctttttcgca cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggtgactc
     5941 actggataaa gcaacgttga aaaaggttgc accgaagcct ggctggctgg aaaccggagc
     6001 ttctgttttt ccggtgctgg ctatcgtatt gattgtacgt tcgtttattt atgaaccgtt
     6061 ccagatcccg tcaggttcga tgatgccgac tctgttaatc ggtgatttta ttctggtaga
     6121 gaagtttgct tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca
     6181 tccgaaacgc ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat
     6241 caagcgcgcg gtgggtttac cgggcgataa agtcacttac gatccggtct caaaagagct
     6301 gacgattcaa ccgggatgca gttccggcca ggcgtgtgaa aacgcgctgc cggtcaccta
     6361 ctcaaacgtg gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc
     6421 gaccagcgga ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc
     6481 cgagcgtaaa gagacactgg gtgatgtgac gcaccgaatt ctgacagtgc cgattgcgca
     6541 ggaccaggtg gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc
     6601 gccgggacaa tacttcatga tgggcgacaa ccgcgacaac agtgcggaca gccgttactg
     6661 gggctttgtg cctgaagcga atctggtcgg tcgggcaacg gctatctgga tgagtttcga
     6721 taagcaagaa ggcgaatggc cgacaggtgt gcgcttaagt cgcattggcg gcatccatta
     6781 atagcccatc ttcgttcacg ttgtcgccgt tatggcgaca acgtgaatta tttatgagat
     6841 aaatctcccg tggctaacga catcccccgt cgttgtgtat agaatattcc cccgaagttt
     6901 aaggttggca cctccaggtt gccacggcac acgaaacagc gttggtcccc atataccggt
     6961 aaactgaaac tgcagcgaag cagttagcag agccatgtat atcaggtctg tttcgtgtgc
     7021 tgaattgttg acgcatttat ttattggtat cgcatgaacc ccatcgtaat taatcggctt
     7081 caacggaagc tgggctacac ttttaatcat caggaactgt tgcagcaggc attaactcat
     7141 cgtagtgcca gcagcaaaca taacgagcgt ttagaatttt taggcgactc tattctgagc
     7201 tacgttatcg ccaatgcgct ttatcaccgt ttccctcgtg tggatgaagg cgatatgagc
     7261 cggatgcgcg ccacgctggt ccgtggcaat acgctggcgg aactggcgcg cgaatttgag
     7321 ttaggcgagt gcttacgttt agggccaggt gaacttaaaa gcggtggatt tcgtcgtgag
     7381 tcaattctcg ccgataccgt cgaagcatta attggtggcg tattcctcga cagtgatatt
     7441 caaaccgtcg agaaattaat cctcaactgg tatcaaactc gtttggacga aattagccca
     7501 ggcgataaac aaaaagatcc gaaaacgcgc ttgcaagaat atttgcaggg tcgccatctg
     7561 ccgctgccga cttatctggt agtccaggta cgtggcgaag cgcacgatca ggaatttact
     7621 atccactgcc aggtcagcgg cctgagtgaa ccggtggttg gcacaggttc aagccgtcgt
     7681 aaggctgagc aggctgccgc cgaacaggcg ttgaaaaaac tggagctgga atgagcatcg
     7741 ataaaagtta ctgcggattt attgccatcg tcggacgtcc gaacgttggc aaatccacat
     7801 tgttgaacaa actgctgggg cagaagatct ccatcacctc ccgcaaagcg caaacaactc
     7861 gtcaccgcat tgtggggatc catactgaag gcgcgtatca ggcgatctac gttgataccc
     7921 ctggcctgca tatggaagaa aaacgcgcta ttaaccgcct gatgaacaaa gcggcgagca
     7981 gctctattgg cgacgttgag ctggtgattt ttgtcgttga aggcacccgc tggacgccgg
     8041 acgacgaaat ggtgctcaac aaactgcgcg atggcaaagc gccggtgatc ctcgcggtga
     8101 acaaagtgga taacgtgcag gaaaaagccg atctgctgcc gcacttacag ttcctggcaa
     8161 gtcagatgaa cttcctcgat atcgtgccga tctctgctga aaccgggctg aatgtcgaca
     8221 ctatcgcagc aatcgtgcgt aagcatctgc ctgaagcgac ccatcacttc ccggaagatt
     8281 acatcaccga tcgctcacag cgttttatgg cgtctgaaat catccgcgaa aaactgatgc
     8341 gtttcctcgg cgctgaactg ccgtactccg tgaccgtgga gatcgaacgt ttcgtctcta
     8401 acgaacgcgg tggttatgac atcaatggtt tgattctcgt tgagcgtgaa gggcagaaga
     8461 agatggttat tggcaacaaa ggggccaaga tcaaaaccat cgggattgaa gcgcgtaaag
     8521 acatgcagga aatgttcgaa gcgcctgtcc accttgaact gtgggtaaaa gtgaaatccg
     8581 gttgggccga cgacgaacgc gcactgcgca gtctcggtta cgttgacgat ctttaagagt
     8641 cactccgatg gaaggctggc agcgcgcatt tgtcctgcat agtcgcccgt ggagcgaaac
     8701 cagcctgatg ctggacgtct tcacggagga atcgggtcgc gtgcgtctgg ttgccaaagg
     8761 cgcacgctct aaacgctcta ctctgaaagg tgcattacag cctttcaccc ctctcttgct
     8821 acgtttcggc gggcgtggcg aagtcaaaac gctgcgcagt gctgaagctg tctcgctggc
     8881 gctgccatta agcggtatca cgctttacag cggtctgtac atcaacgaac ttctctcccg
     8941 cgtactggaa tacgagacgc gcttctctga actctttttc gattacttgc actgcattca
     9001 gtctcttgca ggtgccactg gtacgccaga acccgcgctg cgccgctttg aactggcact
     9061 tctcgggcat ctgggctatg gcgtcaattt tacccattgc gcgggtagcg gcgagccggt
     9121 agatgacacc atgacgtatc gttatcgcga agaaaaaggg tttatcgcaa gcgtcgttat
     9181 cgacaataaa acgttcaccg gaaggcagtt aaaagcgtta aacgcgcggg aatttcctga
     9241 cgcagacaca ctacgcgccg cgaaacgctt tacccgcatg gcgcttaagc cgtatcttgg
     9301 cggtaaacca ttaaagagca gggaactgtt ccggcagttt atgcctaagc gaacggtgaa
     9361 aacacattat gaatgatgag gattgtcatg gctgaattac tgttaggcgt caacattgac
     9421 catattgcaa cgctgcgtaa cgcacgcggt actgcttacc cggatccggt gcaggcggcg
     9481 tttattgccg agcaggcggg agcggacggc attaccgtgc atttacgtga agatcgccgc
     9541 cacattactg accgcgatgt gcgcatcctg cggcagacgc tggatacccg aatgaatctg
     9601 gagatggcag tgaccgaaga gatgctggcg atcgccgttg agacgaagcc acatttttgc
     9661 tgcctggtac cggaaaagcg tcaggaagta acaaccgaag gcggcctgga cgttgcaggg
     9721 cagcgtgaaa aaatgcgcga tgcctgcaaa cgtctgacag atgccgggat tcagatttct
     9781 ctctttatcg acgccgatga agagcagatc aaagctgcgg cagaggttgg cgcaccgttt
     9841 atcgagatcc acaccggttg ctatgctgat gccaaaactg acgccgaaca ggcgcaagag
     9901 ctggtgcgta tcgccaaagc cgcgaccttt gccgctagtc tcggtctgaa agttaacgcc
     9961 ggacacggtc tgacctatca caacgtgaaa gccattgccg ccatccctga gatgcatgaa
    10021 ctgaatatcg gtcatgccat tattggccgt gcagtgatga ccggactgaa agatgcggtg
    10081 gcagaaatga agcgtctgat gctggaagcg cgtggctaat ggcaatatta ggtttaggca
    10141 cggatatcgt agagatcgct cgcatagaag cagtgatcgc ccgatccggt gagcgcctgg
    10201 cacgccgcgt attaagcgat aacgaatggg caatctggaa aacgcaccac cagccggtgc
    10261 gttttctggc gaagcgtttt gctgtgaaag aagccgcagc aaaagcgttt ggcaccggga
    10321 tccgcaatgg tctggcgttt aatcaatttg aagtattcaa tgatgagctc ggcaaaccac
    10381 ggctacggct atggggtgag gcattaaaac tggcggaaaa gctcggcgtt gtaaatatgc
    10441 atgtaacgct ggcagatgaa cgccactatg cttgtgccac ggtaattatt gaaagttaaa
    10501 ataataccgt tctctttaaa aagtaaaacc cccgaactgg taaggaacgg gggttttact
    10561 ttttaacttt aattttgtgc agcatgcacc ctcaacaaca gcgattatgg atcagtgtac
    10621 ttatctttta caagcaactt gcaatcttta gcataaaaac tcaagccttt acgaagaaag
    10681 caatactgaa cggctgcatt ccttgtttaa ggaaaaacga atgactgatt gccgatacct
    10741 gattaaacgg gtcatcaaaa tcatcattgc tgttttacag ctgatccttc tgttcttata
    10801 acacaaggaa acgtacttaa ggtgcgtccg gtgaaccaat cggacgcgcc tttaataact
    10861 ataaataagt gtcttggcag atactatata aattaactta gtgaatgatt atgctaatgt
    10921 catcaattaa ataaatataa tggcgttaag gcttcccagt aatataatta atactctact
    10981 tccagagtag aatattaaat tttatccgcg tggtgcatca gcacaaattt atcccacaac
    11041 tgttcttctg tctcgacatg cgccggatct ttcacaatag tattggggat cgggcacacc
    11101 ttctggcagg ttggtgtctc gtagtgccct acgcattcgg tacacttatc gctgttaatc
    11161 tcgtagatat gatctcccat tgaaatcgcc tcattcgggc attcgggttc acacatatca
    11221 caattaatac agcgtttagt aattagtaaa gacatttcaa tggattaccg ttaaatcatt
    11281 ttaaaatcag taagttgtat cgagtttgta tgctttactg tcattaactt actgtatgtt
    11341 gatccagtgt atttaacctt gataaactca gtccagcaac acaaaaccgc aacacattgc
    11401 attttgtccc gtagaaaaga cttgtatgtg tgagcttgtt ttctgcgcct acgcagataa
    11461 ggattgagaa tgccgcgcac tgtaacacat aatccggata gccccaataa tgacgatgtt
    11521 ttagccgcat ctgaaaaatg ggacgcctgt aaacccccct ataccagcgc acacatgaaa
    11581 atctgtgttg ctgccgccaa aatcatcctc gctgcttccg gcgtggctcg ccgttccaaa
    11641 tacgaaaaag agaactatct ccgtatcgat ttcagcaaag ccggtaaggt tacattttac
    11701 gccgagtttc caaaaaagat gggcctcaaa ggtaaaaagc tcggcgagtg gccggagctc
    11761 gctatccagc tggcgcgcga aaaagcacta ggtatggctg acggtggact gcgggcagag
    11821 tccgtacatg cagcgctgga aatgtaccgg gatgacctca aagccaaagt agcccggcag
    11881 aagctgagcc cggacagttt cacaacctac ggggtgcgta tcgaccggat taaagcaacg
    11941 ttcggcgagc gtgaggtgtt cagcgacgta acatacaatc ggctggtgga agtgctggac
    12001 gagtggatcg ccactcgctc gaacaataac gccctggagt tgtttgccga gctccgtcgg
    12061 ttctggaagt tctgcgcacc tactctttgc aacggccgca atgttgccgc cagtctgcca
    12121 gatgattatg tttcctcccg cgtacagaaa cctaccccca cacggctttt taccgatatt
    12181 gaatcaatcg cccgactctg gctcaatgtt gctgcctgca cctctgtaca ccagaagaat
    12241 gctgttcgct tcatgatcat cactggtgtt cgtccgatta atgtccataa cctgcgctgg
    12301 gactacgttt acgaggaggc tggtgaaatt gtttatccgg aaggggttat cggcatgcga
    12361 ggggctatga aaacacaaaa ggctttccgc ctgccgataa cgcctgagat ccggcggatt
    12421 atcgacgagc agaaagcctg gcgtgattca gttcctgagt gcaacaggga ttatgtattt
    12481 ttgcagctac gtgatccaat gcagccattt tcaaaacgat cactggataa gctggtgaaa
    12541 acatacagcc cggacggggc tgtaaaagga ataaaacatg atgggactgt taaagggaaa
    12601 gacggtgcat ttaatacgat gtgccgtaaa ttccttaaga gcaatgttat tgccttgatc
    12661 gataggaatt aaaaccccaa aaagattaaa aaaacaccac aaaacggatg tttcttcaac
    12721 accacttttg ctcatatgaa cggaaccgac gattaaactg gatggctctg attgattcag
    12781 ggtatgaatg gcggtttttt gctccgtttc cctcaaaatg gacgcaactt tccctctgcg
    12841 gctctcagcc gcaccaccgc atccgggcca gcggctcatg catcaggacc tgctctgcca
    12901 gacggtagcc ccgcttcagc cccgtaaaac gcatctgact cccgcacagc acgcacttca
    12961 gcgggtcaac cttcagtaac ctctgataca tccctctcca ggtgatttgc atcgccgttt
    13021 ttctcactgt ctccgttatg atgtacacca cttcttccag taaccgccgt ttcgccggac
    13081 tcaaaaaacc gtagtacctc accatacgga accccttatc cgccacatgc caggagaacc
    13141 tttccatgaa ctcatctcca ctcatcaaca ggtattcttc ccgttttgtt cggtgactgt
    13201 tgtaacgcag accgatttca tcctgaccgg cataatgctc cagacgactc atcggcaccg
    13261 gtggcttttt caggtaagag ccaaagtaca ccgccacatg gggggcatta tccatcaccc
    13321 gggatacgtt gacattccag ccacggcggt aatgcgtgtc caggaagcga ttccattccc
    13381 gtttactgct tccttctgct gccagcgcat ccggcatcac caggtcaggg tatttccgtg
    13441 acagcaaccg tgttatccgg tagcgccaca tgctcatcac cttacgggcg taaaaatgaa
    13501 gatttttcca ggtgtggccc gacgtcacac caccggcagt tgtcgataaa tggatatgcg
    13561 gatgccactg ctggtcacgc ccccatgtgt ggatcaccgt gaatatcccc ggctccacat
    13621 ctgcctgatg gcagatttcc agtatcacat ccgctgcaat gcggctcatc tctgtcagta
    13681 accaccggtt gtggaacacc agggaccagt actggcaggg aagtgtgaac acaatatgct
    13741 gccacgggca gtcggggacc aggctcagca gatactgtat ccactgtgcg ccagccttca
    13801 ccccgcagtg cgggcaggag cggcttttac accggaagca gacctttttt gtatggcaac
    13861 agtccggtga tgaacagcac cactgtgtat accccatcag tgtggtcccg cacgccatga
    13921 ttttggtcac cgactcaatc accaccggac gtactgccct ttccggctgc ttctccagcc
    13981 agttaagcca gtggtttccc tgctgaaaga tatcggcaaa acggggaagc atcagaaggg
    14041 cggggcgact ccgtccggcc agtgaaccgt gccacactcc gggcagtaca taccgccggc
    14101 gctgataccg gaaagaatgg tcgcaaattc ccgctccgtg cagcgggcga tttccggata
    14161 cccttcgtca tcaacacgta caaaccagaa gaccagcttt ttgtttcccg catccacaaa
    14221 gaacggaata ttcaggtctg cgcagcattc aacggcatcg tcaaaactat caaagcgcag
    14281 aacttctgcg tcttcttcgt caaaaaaatc atcttcgtga agcttcacga catagcgggg
    14341 aagtttgctt ctttgagagg cgggttta
//
LOCUS       DAZPLI010000100        14297 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZPLI010000100 DAZPLI010000000
VERSION     DAZPLI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14297
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.101"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            19..1236
                     /gene="rtcB"
                     /locus_tag="ACHUU3_002874"
     CDS             19..1236
                     /gene="rtcB"
                     /locus_tag="ACHUU3_002874"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR2082891.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRDLAY
                     FMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEEINC
                     HHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCSCSH
                     GAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSDLVE
                     VIYTLRQVVCVKG"
     gene            1240..2256
                     /gene="rtcA"
                     /locus_tag="ACHUU3_002875"
     CDS             1240..2256
                     /gene="rtcA"
                     /locus_tag="ACHUU3_002875"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR2082892.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2299..5004)
                     /gene="malT"
                     /locus_tag="ACHUU3_002876"
     CDS             complement(2299..5004)
                     /gene="malT"
                     /locus_tag="ACHUU3_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFR2082893.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5615..8008
                     /gene="malP"
                     /locus_tag="ACHUU3_002877"
     CDS             5615..8008
                     /gene="malP"
                     /locus_tag="ACHUU3_002877"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR2082894.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLVGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8018..10102
                     /gene="malQ"
                     /locus_tag="ACHUU3_002878"
     CDS             8018..10102
                     /gene="malQ"
                     /locus_tag="ACHUU3_002878"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR2082895.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10147..11463)
                     /gene="gntT"
                     /locus_tag="ACHUU3_002879"
     CDS             complement(10147..11463)
                     /gene="gntT"
                     /locus_tag="ACHUU3_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR2082896.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11824..12399)
                     /gene="nfuA"
                     /locus_tag="ACHUU3_002880"
     CDS             complement(11824..12399)
                     /gene="nfuA"
                     /locus_tag="ACHUU3_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR2082897.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12458..13141)
                     /gene="gntX"
                     /locus_tag="ACHUU3_002881"
     CDS             complement(12458..13141)
                     /gene="gntX"
                     /locus_tag="ACHUU3_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR2082898.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13179..13949
                     /gene="bioH"
                     /locus_tag="ACHUU3_002882"
     CDS             13179..13949
                     /gene="bioH"
                     /locus_tag="ACHUU3_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR2082899.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13978..>14297)
                     /locus_tag="ACHUU3_002883"
     CDS             complement(13978..>14297)
                     /locus_tag="ACHUU3_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR2082900.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3440 a         3565 c         3992 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgggtaac cactttattg aaatctgcct tgatgagtcg
      541 gagcaggtgt ggattatgct gcactccggt tcacgcggaa ttggtaacgc catcgggact
      601 tactttatcg atctggcgca aaaagagatg caggaccaac ttgagacgtt gccgtcgcgt
      661 gatctggcgt actttatgga aggtacggaa tactttgatg attacctaag agccgtggcc
      721 tgggcgcagc ttttcgccag ccttaaccgc gatgcgatga tggaaaacgt ggtaacggca
      781 ttgcagagca ttacgcagaa aacggtcaga cagccacaaa cgctggcgat ggaagagatc
      841 aactgtcacc acaactatgt gcaaaaagaa cagcactttg gtgaagagat ctacgtgacg
      901 cgtaaaggcg cggtgtttgc gcgtgctggt caatatggaa ttattccagg gtcgatggga
      961 gcaaaaagct ttatcgtccg tgggctggga aatgaagaat ctttctgttc gtgcagccac
     1021 ggtgccgggc gggtaatgag ccgtactaaa gcgaaaaaac tgttcagcgt ggaagatcaa
     1081 attcgtgcca ccgcgcatgt ggaatgccgt aaagatgccg aagtgatcga cgaaatcccg
     1141 atggcgtata aagatattga tgcggtgatg gcggcacaaa gcgatctggt ggaagttatc
     1201 tataccctgc gtcaggtggt gtgcgtaaaa ggataaatga tgaaaaggat gattgcgctg
     1261 gatggcgcac agggcgaagg tggcgggcag atcctgcgct cggcgctgag cctgtcgatg
     1321 ataaccggcc agccatttac catcaccagc attcgtgccg ggcgggcgaa accggggctg
     1381 ttgcgccagc atctgaccgc ggtaaaagcg gctgcggaaa tttgtagggc aacggtggaa
     1441 ggcgcggagc tggggtcgca gcgtctggtc ttccggcttg gcaccgtgcg cggcggcgag
     1501 taccgctttg ctatcggtag tgccggaagc tgtacgctgg tgctgcaaac ggtgctgccc
     1561 gcgctgtggt ttgccgatgg accttcgcgt gttgaagtga gcggaggcac cgataacccg
     1621 tcggccccgc ctgcggattt tatccgccgg gtgctggagc cgctgctggc gaaaatagga
     1681 attcatcagc aaaccacgct gttacgtcac ggtttttatc ctgccggagg cggcgtggtg
     1741 gcaacggaag tctcgcccgt ggcatcgttt aacaccttgc aacttggcga gcgcgggaac
     1801 attgtgcaga tgcgtggaga agttctatta gctggtgtgc cgcgccatgt tgctgagcgt
     1861 gaaatcgcta cactggcggg gagtttttcc ctgcatgaac agaatattca taacctgccg
     1921 cgcgaccagg ggccgggtaa taccgtctcg ctggaagtcg aaagtgaaaa tatcactgaa
     1981 cgctttttcg tcgtcggtga aaagcgcgtc agtgccgagg tggtagccgc gcagttagtg
     2041 aaagaggtga aacgctacct ggcaagcccg gcggtagtgg gggaatatct cgccgaccag
     2101 ctggtgctac cgatggcgct ggcgggcgcg ggagaattta cggtcgccca tccttcatgc
     2161 catctgctga ccaatatcgc cgtggtggag cgtttcttgc cggtgcggtt tggtttgata
     2221 gaaacagatg gcgtaacgcg ggtgagtatt gaatgatgta aagatgtaaa ccggatccaa
     2281 tgcattatcc ggctaaactc acaccccata tcccatcatc ttcagcagtt tctgcgcgtg
     2341 ttgtaccgcg gcctgacggt gggcgacgcc gagtttttgg tacagattgc ggatatgcgt
     2401 cttgatggtg gttgccgcca cttccagctc gcccgcaatt tgctcattgc tgtaaccgga
     2461 gtagatcagc cccaggacct gccattcacg ttgggtcaac gggctggtgc ggatcagttc
     2521 tggcacttcc ggatgattca gcagacgttc aacgaaattc tcatcgaaat gggcgaattt
     2581 atgccgatga tgttgattga tttctcgcag aatacgctgc gcgcgatgct gttccagttc
     2641 cggcagcgta ttgagttgaa tcagctggcg cagttgttgc gccatcgctt cgccttcgat
     2701 gacaaaatga ctgataaatc cggtgcgatt cgccagtttt aatgcgtcca gcaacacgcg
     2761 ctgggcgtca cttttacgtc cggcctgcca gtacagttga ttaagcagca acaggttacg
     2821 gttgagatcg ctcatcaacc gcagactgcg ggcattttca ttgagttctt cgagaacaat
     2881 ttccgcaggt tcaaactcgc ccagcaagat ttgtgcgcgg gcaatgttgc gccattgacc
     2941 ttgcaggaag tggttgttcg caaactccgg tttagccgta tgacgcaacc agttggcagc
     3001 ggcggcttta tcgccggtca tttgccagta aatcacccgg actttgttgg cgttagagat
     3061 ccagtcgctg tgatatttgc cattccccag caggttttcc agacggttca gctggctacg
     3121 ggcgttatct aaatcaccac gggccagcga gcattgaatc aacattgcca ggcactgaag
     3181 ctgttgctgt ggctgataag acgacaagac ttcaatcccg ctacgcgccg acgcttcggc
     3241 ttcatccagc cgtgcccagg cccataacag ttgtgctcgg atgcgcacca gaaactcatg
     3301 catcggcagc tgttccagat gctgctcgtt gatcagctgg aacgtttttt cctgtgtttc
     3361 ccacgcggtt tgcaggaacc cttgggcaaa cagaatttca ctttgctgga ttaaactcca
     3421 taaggcgtag tgccagacgt cgtgctggcg tgccatctgt tcggtttgct gcattagcgc
     3481 cagtgagcgg gtcaattcgc ctttgcagtg cagcacttca cccagcaccg aggttgctac
     3541 aatgcggcta tagaaccagc ccggtggcag ctcttccagt gccagttttg ccagccgttc
     3601 cgcttcatcc ggattaccat cgttaatcgc cacctgggcg cgcagagcgt taaattctgc
     3661 gtgcatggtg ccttctctga tgtccttgat ttcatgttca gcacgggcta gcagggtgtt
     3721 aacttcgcca tagcgatgtt ggctttgcat tagccacgcc tgcaataaca ctaactgcgg
     3781 attttccagc aaactgtccc acggcagggc cttaagcgac tcttccagca gcgacagttc
     3841 gctatggttg aacaaactcc aggcgtgatt aagcagaata tcgcgcagca tcagcgcatc
     3901 gcctgccgcc agcgcatgat gaatcgcttc gctgggaaat ccctgggcca tccagctttc
     3961 tgcggcggca cggtggattt ccggcagctc cgccgccagt tcccactggc agcgctggcg
     4021 caggaagttg ccaaacagcg ggtgatagca gaaccactcg ccggtatcat ccatccgctg
     4081 taaaaacagc ccctgacgct caatctcttc gagacgcatt tgcccgtttt cttcgccagt
     4141 cacacgggtg atgagggcat cgttcattga gcgcaaaatg gcgcttttca acagaaaatg
     4201 gcgcgttgcg agatcgacgt tatccaaaac ctcatcgacc agataatccg aaagatggct
     4261 ggcattgatt cccgccaggc ggcgtgccga cttatgggct gagtgggtat tctgccgggc
     4321 ggagagggcg attagctgta gtgccgtcgc ccaaccggaa acgtcatcgc aaatccaact
     4381 gctttctgcg gcttcaatcg gcgatgacag acggcaatca aaaaactgct tcgcttcctg
     4441 atgggtaaat gccagttgct gactgccaat ttccagcagt tggtcacgaa cacgcagatt
     4501 ggcaatgccc agttgcggaa ggttgcgtga caacaccacc agggtgagat tttctggttg
     4561 atggcgaata aagaagcgca ttgactcgtg gatcactgga ttagtgatca gatgatagtc
     4621 atcgatgacc agataaagtg ggctatgcca ttccgccagc tcaatgaaaa gctgggcgaa
     4681 gagtgacgtc aggctggcat attgccgttt ttgcgccatc gtctcacata tcgcacagtg
     4741 accgttggtt gcctgctgca cggcggcaat gagatagctg gcgaaacgct cttgctggtt
     4801 atcaccttca tccagcgagt accagccgat atcgtttttg cctgccgccc actgggaaat
     4861 gagggtggtc tttccgtagc ccgcaggact cgtgatcagc gccagccgga agttgttcgc
     4921 gccggaaagt ttagccagca ggcgctcacg aaccacggta tggtcgagtc gaaccggacg
     4981 acttagtttt gacggaatca gcatagttaa tcacttcact gtggaaaatg aggaaatatt
     5041 atttttttgc gcttcgtaat taatggttat aaggtcggcc agaaaccttt ctaatgcaag
     5101 cgatgacgtt tttttatgtg tctgaatttg cactgtgtca caattccaaa tctttattaa
     5161 caactcacct aaaacgacgc tgatccagcg tgaatactgg tttcccttat gttcatcaga
     5221 ttcatttaag caagggtttc ttcttcattc ctgatgaaag tgccatctaa aaagatgatc
     5281 ttaataaatc tattaagaat gagatggagc acattggata ttttacttgt gaaactgttt
     5341 cactccttta cttaatttat agagttacct tccgcttttt gaaaatacgc aacggccatt
     5401 ttttgcactt agatacagat tttctgcgct gtattgcatt gatttgctgc taatcctgtg
     5461 gtttgcacta gctttaagtg gttgagatca catttccttg ctcatccccg caactcctcc
     5521 ctgcctaatc ccccgcagga tgaggaaggt caacatcgag cctggcaaac tagcgataac
     5581 gttgtgttga aaatctaaga aaagtggaac tcctatgtca caacctattt ttaacgataa
     5641 gcaatttcag gaagcgcttt cacgtcagtg gcagcgttat ggcttaaatt ctgcggctga
     5701 aatgactcct cgccagtggt ggctagcagt gagtgaagca ctggccgaaa tgctgcgtgc
     5761 tcagccattc gccaagccgg tggcgaatca gcgacatgtt aactacatct caatggagtt
     5821 tttgattggt cgcctgacgg gcaacaacct gttgaatctc ggctggtatc aggatgtaca
     5881 ggattcgttg aaggcttatg acatcaatct gacggacctg ctggaagaag agatcgaccc
     5941 ggcgctgggt aacggtggtc tgggacgtct ggcggcgtgc ttcctcgact caatggcaac
     6001 tgtcggtcag tctgcgacgg gttacggtct gaactatcaa tatggtttgt tccgccagtc
     6061 ttttgtcgat ggcaaacagg ttgaagcgcc ggatgactgg catcgcagta actacccgtg
     6121 gttccgccac aacgaagcac tggatgtgca ggtagggatt ggcggtaaag tgacgaaaga
     6181 cggacgctgg gagccggagt ttaccattac cggtcaagcg tgggatctcc ccgttgtcgg
     6241 ctatcgtaat ggcgtggcgc agccgctgcg tctgtggcag gcgacgcacg cgcatccgtt
     6301 tgatctgact aaatttaacg acggtgattt cctgcgtgcc gaacagcagg gcatcaatgc
     6361 ggaaaaactg accaaagttc tctatccaaa cgacaaccat actgccggta aaaagctgcg
     6421 cttgatgcag cagtacttcc agtgtgcctg ttcggtagcg gatattttgc gtcgccatca
     6481 tctggtgggg cgtaaactgc acgaactggc ggattacgaa gttattcagc tgaacgatac
     6541 ccacccaact atcgcgattc cagaactgct gcgcgtgctg atcgatgagc accagatgag
     6601 ctgggatgac gcttgggcca ttaccagcaa aactttcgct tacaccaacc ataccctgat
     6661 gccagaagcg ctggaacgct gggatgtgaa actggtgaaa ggcttactgc cgcgccatat
     6721 gcagattatt aacgaaatta atactcgctt taaaacgctg gtggagaaaa cctggccggg
     6781 cgatgaaaaa gtgtgggcca aactggcggt ggtgcacgat aaacaagtgc atatggcgaa
     6841 cctgtgtgtg gttggcggtt tcgcggtgaa cggtgttgcg gcgctgcact cggatctggt
     6901 ggtgaaagat ctgttcccgg aatatcacca gctatggccg aacaaattcc ataacgtcac
     6961 caacggtatt accccacgtc gctggatcaa acagtgcaac cctgcactgg cggctctgtt
     7021 ggataaatca ctgcaaaaag agtgggctaa cgatctcgat cagctgatca atctggaaaa
     7081 attcgctgat gatgcgaaat tccgtcagca atatcgcgag atcaagcagg cgaataaagt
     7141 ccgtctggcg gagtttgtga aagttcgtac cggtattgag atcaatccac aggcgatttt
     7201 cgatattcag atcaaacgtc tgcatgagta caaacgccag cacctgaatc tgctgcatat
     7261 tctggcgttg tacaaagaaa ttcgtgaaaa cccgcaggct gatcgcgtac cgcgcgtctt
     7321 cctcttcggc gcgaaagcgg caccgggcta ctacctggcg aagaatatta tctttgcgat
     7381 caacaaagtg gctgacgtga tcaacaacga tccactggtt ggcgataagt tgaaggtggt
     7441 gttcctgccg gattattgcg tttcggcggc ggaaaaactg atcccggcgg cggatatctc
     7501 cgaacaaatt tcgactgcag gtaaagaagc ttccggtacc ggcaatatga aactggcgct
     7561 caatggtgcg cttactgtcg gtacgctgga tggtgcgaac gttgaaatcg ccgagaaagt
     7621 cggtgaagaa aatatcttta tttttggtca taccgtggaa caagtgaagg caattctggc
     7681 caaaggctac gacccggtga aatggcggaa gaaagataag gtgctggacg cagtattgaa
     7741 agagctggaa agcggtaaat acagcgacgg cgataagcat gccttcgacc agatgctgca
     7801 cagtatcggc aaacaaggcg gcgatccgta tctggtgatg gcggatttcg cagcctatgt
     7861 tgaggcacaa aagcaggtgg atgtgctgta ccgcgaccag gaggcctgga ctcgcgcggc
     7921 gatcctcaat accgcccgct gcggtatgtt tagctcggat cgctctattc gcgattatca
     7981 ggctcgtatc tggcaggcaa aacgctaagg aagctcgatg gaaagcaaac gtctggataa
     8041 tgccgcgctg gcggcgggga ttagccccaa ttacatcaat gcccacggta aaccgcagtc
     8101 gattagcgcc gaaaccaaac ggcgtttgct tgacgcgatg catcaacgta ccgccacgaa
     8161 agtggcggta acgccagtcc cgaatgtcat ggtttatacc agcggcaaaa aaatgccgat
     8221 ggtggtggag ggcagcggcg aatatagctg gctgctgacc accgaagaag gaacacagta
     8281 caaaggccat gtaacggggg gcaaagcgtt caatctaccg acgaagctgc cggaaggtta
     8341 tcacacgctg acactcaccc aggacgacca gcgcgcgcat tgccgggtga ttgtcgcccc
     8401 gaaacgctgt tacgaaccgc aggcgttgct gaataaacaa aagctgtggg gtgcctgcgt
     8461 tcagctttat acgctgcgat cggaaaaaaa ctggggtatt ggggattttg gcgatctcaa
     8521 agcgatgctg gtggatgtgg caaaacgtgg cgggtcgttc atcggcctga acccgattca
     8581 tgcgctctat ccggcaaatc cggagagtgc cagcccatac agcccgtctt ctcgccgttg
     8641 gctgaatgtg atttatatcg acgttaaagc cgtcgaagat ttccatctta gcgaagaggc
     8701 tcaggcctgg tggcagttgc cgaccacgca acagacgctg caacaagcgc gggatgccga
     8761 ctgggtcgat tactccacgg ttaccgccct aaaaatgaca gcattacgaa tggcgtggaa
     8821 aggtttcgcg caacgtgatg atgagcagat ggccgcgttt cgccagtttg ttgcagatca
     8881 aggcgacagc ctgttctggc aggcagcctt tgatgcgcta catgcccagc aagtgaaaga
     8941 ggacgaaatg cgctggggct ggcctgcatg gccagagatg tatcagaacg tggattcacc
     9001 agaagtgcgt cagttctgcg aagaacatcg taatgacgtc gatttttatc tctggttgca
     9061 gtggctggct tacagccagt ttgccgcctg ctgggagata agccagggct atgaaatgcc
     9121 gattggcttg tatcgtgatc tggcggttgg cgtagcggaa ggtggggcgg aaacctggtg
     9181 tgaccgtgaa ctatattgcc tgaaagcgtc ggttggcgcg ccgccggata tcctcggccc
     9241 gttggggcag aactggggat taccgccaat ggacccgcat atcatcaccg cgcgtgccta
     9301 tgaaccgttt atcgagctgt tgcgtgccaa tatgcaaaac tgcggcgcat tacgaattga
     9361 ccatgtgatg tcgatgctgc gtttgtggtg gataccgtat ggcgagacgg cagatcaggg
     9421 cgcgtatgtt cactatccgg tggatgatct gctctcgatt ctggcactcg aaagtaaacg
     9481 tcatcgctgt atggtgattg gtgaagatct cggtaccgta ccggtagaga ttgtcggtaa
     9541 gctgcgcagc agcggtgtgt actcctacaa agtgctctat ttcgaaaacg accacgagaa
     9601 gacgttccgt gcaccgaaag cgtatccgga gcagtcgatg gcggttgcgg cgacacatga
     9661 cctgcctacg ctacgcggtt actgggagag cggggatcta acgctgggca aaaccctggg
     9721 gctgtatccg gatgaagtgg tactgcgcgg tctgtatcag gatcgcgaac tggcgaagca
     9781 agggctgctg gatgcactgc ataaatatgg ttgtctgcca aaacgtgccg ggcataaggc
     9841 atcgttgatg tcgatgacgc cgacgctgaa ccgtggtttg cagcgctaca ttgccgacag
     9901 taacagtgct ctgttaggac tacagccgga agactggctg gatatggccg aaccggtgaa
     9961 tattcctggc accagttacc agtataaaaa ctggcgacgc aagctttccg caacgcttga
    10021 gtcgatgttt gccgatgatg gcgtgaacaa gttgctgaag gatttggaca gacggcgcag
    10081 agctgcagcg aagaagaagt agagtgcggt tgatgccgga tgcgatgcat ccggcagtca
    10141 gtgttatcaa atcaccatat tcagcagcag acagcctacc agaccgcaca ccgagataat
    10201 ggtttccagc atcgaccagg atttgatggt ctcgccgata gtcaggttaa agtactcttt
    10261 gaacagccag aagcccggat cgttcacatg agagaaaatc acactaccgg aacccaccgc
    10321 aataaccatc agctctgggc taacacccgt cgttgcaatc agcggtgccg cgataccacc
    10381 cgcagtgatt gccgcaacgg ttgcggaacc cagcgcgata cgcagtacgg cagcaatcga
    10441 ccaggccatc agcagcggag aaatgttggt ttcgtgcatc atggaagcaa tgtatttgtc
    10501 cacgccgctg tctaccagca cctgcttgaa cgcaccgcca ccaccgatga tcaacagcat
    10561 catcgcaatg attttgatgg aagaaaccag cgtgtcgtta atctgatcca ttgaacgacc
    10621 acggttcaga ccaaaggtga acatcgcaat cagcacggca atcagcgttg ccattaccgg
    10681 gtcaccgagg aactccgcta ccggcaggaa agcgtgacct ttcggcagga tcatttcggc
    10741 aatcgcacgc atcgccatca gcacaaccgg caccagagaa gtccagacgc tgacgccaaa
    10801 gctcggcatc tcttcttcgc tgaaggtttt cgcgctgtag agaccttccg ggattggctt
    10861 atcgatacct ttcaacacgc gagcgtaaac cggaccggcg agaatcacgg tcgggattgc
    10921 cagaatagta ccgtacagca gggttttacc catatcggca ttgaaaatgg tggcaatcgc
    10981 agtcgggccc ggatgcggtg gcaggaagcc gtgggtcaca gacagtgcag ccgccattgg
    11041 tacaccaaca tacagcagtg gaatattcgc ggaagccgcg atggtaaaca ccagcggcag
    11101 catcagcacg aagcccactt catagaacag ggcaaaacca acggtaaaac cggttaacac
    11161 caccgcccac tggatgtgct ttttaccaaa tttggcaatc agcgtggtgg cgatacgttg
    11221 tgcgccaccg cagtctgcca gcattttgcc cagcattgcg ccaaaaccca tgatcagggc
    11281 aaggctaccg agcgtcccgc cgacaccgac tttgatggag tcaataactt tatccagcgg
    11341 cattccttgc attaatccaa cagcaagcgc cacgaggacg agagcgatga agccgttcat
    11401 tttgaagcgg atcatcagga gcaacaacaa gattacaccg atagcaacaa tgactaatgg
    11461 catgatttac ctggcctttc atttgttatg ggtaacgtca attttctgac gacaaactct
    11521 aattatccca atcgggaaca gagatattgc ggcaccacga ctgataccca ctaaaactaa
    11581 ttattgtagt cagatgtcag gagtatgttt ggtacccatg tgaatgatac gggtaacatt
    11641 tggcgtttga gaatcaccag agcggggtaa atttaaatta tgagaggttg gtcatattat
    11701 cgcggggaaa cgaagggagg gatttgccaa agcaatgctg tgccaacgtc tggcacatgt
    11761 tcaacgtagg cccgaaatga cgctttagcg tcgcatcggg caatctacaa aagaggggat
    11821 aacttagtag taggagtgtt cgccgcgctg gtgttcggtg agatcgcgca cacctttcag
    11881 ctccgggaat tcgttcagca gctgcttctc gatcccttct ttcagcgtca catcgaccat
    11941 ggaacaaccg ttacagccgc cgccaaattg cagaatggcg taaccgtctt cggtgatttc
    12001 catcagcgaa acgcgaccac cgtgaccagc aagctgtggg ttgatctgcg actgcagcat
    12061 atactccacg cgctccatca gcggtgcatc gtctgccact ttacgcattt tggcgttcgg
    12121 ggctttcagc gttaactgag aacccaactg gtcggtaaca aagtcgatct ctgcatcttc
    12181 caggtatggt gcgcttaact catcaacata cgcggtcagc aggtcaaatt tcagggcagt
    12241 gtcggtggct tccacggcat ccggcggaca ataagaaaca ccacattcag cgttaggcgt
    12301 gccagggtta atcacaaata cgcggatttg tgtcccttct tcctgatttg ccagcagttt
    12361 ggcaaagtgc gcttgtgcag catcggaaat acggatcata gtaatggcct aatagttgac
    12421 tattttagtt ggttataata cgcccatcat cgaggctcta caaggttcga caaaggcacc
    12481 agacctggac agtcgccgca ccattgcgta aaagcaactg cgcaatctct gcgacggtac
    12541 ttccggtggt aacgacatca tccacaatca ccatatggcg accttgcacg ggcaattcaa
    12601 gacgaaaggc atttttcagg ttgcgcttgc gcagccgggc actgagaaaa tgctgggtcg
    12661 cagtggcccg tgtacgtgtg acggcttcgc tatcccattg gcagtgcaac cagcgtgata
    12721 acggctgaca cagcaaatcg ctctgattaa atccccgacg ccagtgacgc cgctgccata
    12781 acggaacgct gatgatgcga tccggcaatt gcaacccggt ggtgcgacga gcgtgtaaga
    12841 cttccaatag taacagacgt gacagggcgc tggcgatttc actgcgccgg gaaaatttaa
    12901 gctggtggat gagcagactt aacggcggcg catagtcggc aaccgtgacc agtctttgcc
    12961 agggcggcgg tttttgcagg cagcgaccgc agggaagatg ggagtgtgtg gcgggtaatc
    13021 cacattgtgg gcataacgtt ttatctgtgc gggtggcgcg tgaacagacc gaacaaatcc
    13081 cccaatgacc taacgccagt ggcattcggc atagccagca taatcccggt actgttagca
    13141 tatgttcatc cttgtaagtc aaaagagaac aatagcggat gaataacatt tggtggcaga
    13201 ccaaaggtca ggggaatgtt catcttgtgc tgctgcacgg atggggactg aatgccgaag
    13261 tgtggcgttg cattgacgag gaacttagct cgcattttac gctgcacctt gttgatctgc
    13321 ccggcttcgg gcgcagccag ggatttggtg cgctgtcact tgctgatatg gccgaagccg
    13381 tgctgcaaca ggcacctgat aaagccattt ggttaggctg gagtctgggc gggctggtgg
    13441 caagccagat tgcgttaacc catcccgagc gtgttcaggc gctggtcacc gtggcgtcgt
    13501 caccttgttt tagtgctcgt gacgattggc cggggataaa accggacgtg ctggcgggat
    13561 ttcagcagca actcagtgat gattttcagc gtacagtgga gcggttcctg gcgttacaaa
    13621 ccatggggac tgaaacggcg cgccaggatg cgcgggcgtt gaagaaaacc gttctggcgt
    13681 taccgatgcc ggaggttgac gtgcttaatg gcgggctgga aatcctgaaa acggttgatc
    13741 tccgtcagcc gctgcaaaac gtgcccatgc cgtttttgcg attgtatggc tatctcgatg
    13801 gtctggtgcc gcgcaaagtg gtgccgatgc tggataaact ctggcctcgc agcaaatcat
    13861 atatcttcgc caaagcggcc catgcgccgt ttatttcgca tccggtcgag ttttgtcacc
    13921 tgctggtggc gttgaagcag agggtgtagg caacttttga aatggcgaga cagaaattta
    13981 ctggctcgcc gcagccaact cttcttctga caccccggta aagcgcatga tgtccgcaag
    14041 agggactccg gattcaagca ttattttggc tatatgcagg gctttggatt gttccccctc
    14101 ctgccgcaat ctttccgcaa ttgtcattaa actctccttg tgtttcggtg aacgttcggc
    14161 aacgccgtcg ataaaatcgt taaaacgtac cgcgtcgccg gtttgcagta tgtaattaaa
    14221 cagccctttg atttgtctgt cattagcgta tccactactt aataagcagg ccatttgctc
    14281 taccagcccc atcaggt
//
LOCUS       DAZPLI010000101        14267 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZPLI010000101 DAZPLI010000000
VERSION     DAZPLI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14267
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.102"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..153
                     /locus_tag="ACHUU3_002884"
     CDS             <1..153
                     /locus_tag="ACHUU3_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR2082901.1"
                     /translation="RRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEGTSGSNRYGND
                     PKFGSN"
     gene            complement(204..1096)
                     /gene="yhaJ"
                     /locus_tag="ACHUU3_002885"
                     /pseudo
     CDS             complement(204..1096)
                     /gene="yhaJ"
                     /locus_tag="ACHUU3_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            1201..1902
                     /locus_tag="ACHUU3_002886"
     CDS             1201..1902
                     /locus_tag="ACHUU3_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR2082902.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1925..2089
                     /gene="yhaL"
                     /locus_tag="ACHUU3_002887"
     CDS             1925..2089
                     /gene="yhaL"
                     /locus_tag="ACHUU3_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR2082903.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2223..3533)
                     /gene="cyuA"
                     /locus_tag="ACHUU3_002888"
     CDS             complement(2223..3533)
                     /gene="cyuA"
                     /locus_tag="ACHUU3_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR2082904.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3561..4892)
                     /locus_tag="ACHUU3_002889"
     CDS             complement(3561..4892)
                     /locus_tag="ACHUU3_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR2082905.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGAFYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5167..6531)
                     /gene="tdcG"
                     /locus_tag="ACHUU3_002890"
     CDS             complement(5167..6531)
                     /gene="tdcG"
                     /locus_tag="ACHUU3_002890"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR2082906.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6603..6992)
                     /locus_tag="ACHUU3_002891"
     CDS             complement(6603..6992)
                     /locus_tag="ACHUU3_002891"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR2082907.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7006..9300)
                     /gene="tdcE"
                     /locus_tag="ACHUU3_002892"
     CDS             complement(7006..9300)
                     /gene="tdcE"
                     /locus_tag="ACHUU3_002892"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR2082908.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9334..10542)
                     /gene="tdcD"
                     /locus_tag="ACHUU3_002893"
     CDS             complement(9334..10542)
                     /gene="tdcD"
                     /locus_tag="ACHUU3_002893"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR2082909.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10568..11899)
                     /gene="tdcC"
                     /locus_tag="ACHUU3_002894"
     CDS             complement(10568..11899)
                     /gene="tdcC"
                     /locus_tag="ACHUU3_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR2082910.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11921..12910)
                     /gene="tdcB"
                     /locus_tag="ACHUU3_002895"
     CDS             complement(11921..12910)
                     /gene="tdcB"
                     /locus_tag="ACHUU3_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR2082911.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13009..13947)
                     /gene="tdcA"
                     /locus_tag="ACHUU3_002896"
     CDS             complement(13009..13947)
                     /gene="tdcA"
                     /locus_tag="ACHUU3_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR2082912.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3720 a         3821 c         3329 g         3397 t
ORIGIN      
        1 cgtcgcttac acgataccga ccgttcaggt gcctgggcct tgctcttttt tgttccgttt
       61 attggctggc tcgtgcttct ggtttttttc tgcacagaag gtacttctgg cagcaatcgt
      121 tacggaaacg atccgaagtt tggttcaaat taatcttaga attggggcga tatttcgccc
      181 ctttttatta acaataataa atattatttt ccgctaaaaa gtttgggaat ttcccgcaga
      241 caccaggatt tcgcttcccc catactgtca cgacgccagg ccataataat atcgatctca
      301 ctggtcgatt ccgggctgac gacacgcaac cgcccttccg caatatcttt ttcgaccatc
      361 ggatacggca tcgtcgccac gccaagcccc gccagtaatg cctgacgttt atcttcaatc
      421 gtgctcaccg ttaagcgcgg ctgtttgtcc agcagttgta cggtcaacac cgggcgctca
      481 cgagcggtat ccgctaccgc aattccacga tatttcacgc gcgtcacttc agataacggt
      541 tccggctcct gatgaatcgg gtgatcaggc gcggcaacgt agacgttcat taacgtatag
      601 agcttgcgcg agttgatctc cgacgaggaa cgaaaatgca tatccggcgc gataacaata
      661 tccgcccgcc cctgctccag tcgttcctac gcccccgcca gcacttcggt gatgattgcc
      721 agttgggtat tggcttttgc cgccagtttg tcgattaacg ggaaaaaggc aggtgtcggt
      781 accagcgctt cggtcacaat ggtgagatgc gtttcccaac cgcgcgcgag ggcttccgca
      841 tcggtagtca gtttatctgc ggcttccagc aaaacgcgcc ctcgctccag caacatcctc
      901 ccgacattgg tgaatttggt gcgatggccc gagcggtcaa acagcaccac atccagctct
      961 tcttccagtt tttgcatggt gtagctaagt gcggaaggca cgcgtcccag ctcatccgcc
     1021 gccgccgcaa aactgccccg gcgatcgcat ccataacccg tagtgcttcc agcgttaatg
     1081 ccctttcttt ggccatttcg ttctcattca ggaaatttga acataccggg cagaatatct
     1141 ggctaacaat gtagcgtcca gccccttacc ataaaaggaa gtaaagagag gtcaagaatt
     1201 atgattacta cccgaactgc caggcagtgt ggacaagcag actacggatg gttgcaggcc
     1261 cggtatactt tttcctttgg acactacttc gacccgaaat tgttaggcta tgcctccctg
     1321 cgtgtgctta accaggaagt gctggcccca ggtgccgcct ttcagccgcg aacctatccc
     1381 aaagtcgata ttttaaatgt gattctggat ggagaagcag agtatcgcga cagcgaaggc
     1441 aatcatgttc aggccagcgc cggtgaggcg ttgctgctct ctacccagcc gggtgtcagc
     1501 tacagcgaac acaatctcag caaagacaaa ccgttaacac gaatgcagct ttggctggac
     1561 gcctgcccgc agcgagagaa tccgctgatt caaaaactgg cgcttaatat gggcaagcag
     1621 caattaatcg cctcgccaga gggtacgatg ggaagcctgc aattacgcca gcaagtgtgg
     1681 ctgcaccata tcgtgctcga caaaggcgaa agtgcgaatt tccagttgca tgggccacgc
     1741 gcgtatttgc aatcgattca cgggaaattt catgcgctta cgcatcatga agagaaagcg
     1801 gcgctgacct gcggtgatgg ggcgtttatt cgtgacgagg ctaacattac gctggttgcc
     1861 gattccccac tgcgcgcttt gctgatagat ttgcctgtct agttgttttt acagggagat
     1921 gatgatgagt aaaaaatcgg ccaaaaagcg ccagccagtg aagcccgtgg tggcgaaaga
     1981 acctgctcgc accgccaaaa attttggcta tgaagagatg ttgagcgagc tggaagccat
     2041 cgtcgcggat gctgaaacgc gtttagccga ggatgaagct accgcgtaaa gacaggttgc
     2101 cggatgcggc gaaaacgcct tatccggcct acaaaatcca acaacattaa cgaattaaac
     2161 aacccgtagg ccggataagg tgcgccagca tcgcatccgg cacaatactc aaaacctggc
     2221 gtttatctgg ccttgctcgc cataatctcg ataatctgcc gatccgtttg ctgcatcgaa
     2281 tggcttgcta acgcacacag gttggcaatc gactgctcaa catcatgcgc cacgatccct
     2341 tcattgccgg tcacggcggt atcatccagc gccattaaca ccgctttcca cgcagccgaa
     2401 gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac catcgcaaat catgccgctg
     2461 acatcgccga tcatactgct gatcgccatc gagatagttt cataacgccc atccaccagc
     2521 catgccatcc cggcggcggc ccccattgct gcggtcgttg cggcacaaag tgcagacaaa
     2581 cgcggtaact ggttatggat gtaaattgcg ctcaaatgag aaagcatcag cgcacgcgcc
     2641 agtcgttcat catccgctcc gaagtgttct gctacaacca ccacaggcat tgttgcggtg
     2701 atcccctggt tacccgagcc ggagttactc atagccggaa gcgtagcgcc gcccatacgc
     2761 gcatcggatg ccgcgctggt acgaatcaca atggatgaag agagatcttt cgccagcaaa
     2821 ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca gcccccactt accgctcaaa
     2881 ccttcctgcg ataacgcaca atttagcttc gcggaatcga gaataaagcg gatcgccgca
     2941 aacgggactt cattgacgaa cttcaggatc tcagccagcg tcgttctgga aagcaccgtc
     3001 agcggagact cttgctcgcc ctctgccaca cacgcctgct gggtaaacac cacaccattg
     3061 tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga tggtgacaca cgcccacttc
     3121 tcaccgttcc agactttggc gcgtgagaag aggatttcat tgcaaggttc ctggatctta
     3181 acggagactt tccccgccgc cagcagtgct ttggcatcgg caattgcctg cgcagttgcg
     3241 tctttcagca cttccagccc ggcgttggca tttccaccta acgcccccag cgccgccgca
     3301 atcggcagcc ccaccattcc cgtgccggga acggtgacgc ccagaccgtt cttcatcaga
     3361 tttggcgaaa cccaggcttc tacacgttca accggacctt ccagttctgc cgcagcaacc
     3421 gccgccgcca gcgccagtga aatcggttca gtacatccca gcgccggttt tacttcctcc
     3481 tgaacggcga ggatgtaacg ctgccataac ggatttaaag tcgaatcaaa catattaaaa
     3541 accttaaaat ttcaggtaaa tcaagaaaat gccaggaacg gagaaacaca aagcaacaaa
     3601 ccagtgacga taatcaggta cagagacatc cctttgtatt tgtgcaatgc cggtactttg
     3661 taaaccagcc acgccgggat caggcaccct accatgccga aaatcgggct acagatagag
     3721 gtgaagctca acaccggtgc gttcagtacg atagcgctcc aggccagcaa aatggcgaaa
     3781 atcatgatgc cgcgctgaac aagattttcg ttaatcttct cggcaggcat cttgcgacgc
     3841 aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc ctaaatagac gccaaagaat
     3901 gcagtcatga cagcaaagat attgagaatg acgctgacca ctttcaccca cgctgcgccg
     3961 tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa tattctgctc gtaggctttt
     4021 accgcttcgt catgccccat cgccagcgtg aacgacacgg cgtagaaaaa gacggtgaca
     4081 aacaaaatgc caaacgcgat attcattgcc cgcaatgctt tatgccgcgc cacttcaata
     4141 gatttttccc gcgagcgata agagatcacc atcgggctta acgtctggat aaacaaaatc
     4201 gacgtcaggg taaacggcag cgtaataatg gcgtttttca ccagcagccc cagcggcggt
     4261 agcgaaccga cgttatacag atgccacatt cccaccatcg acacgcccag cgccgcgacc
     4321 accagcagct tggtcagcac catgccggtc gaaattttga ataacaattt ctcgccgcgt
     4381 gaagagatcg ccaccagaat gcaaatcagt accagaccat aaaagggact gtctgacagc
     4441 aacccttccg tcacgccgaa ggtatgcaga taggaagcac tatcgttggt gatggcggtg
     4501 gaatagacga acatccaaat caccagcatt acgaaataga acgcacctaa caggatgccc
     4561 cagtttttac ctaaataacc gctaatgaca ctcgggtaat ctttacattc tggtgattct
     4621 gccagcgtat taataaacaa ccgctgaaac agatacattg ccgggtaacc aatcaccgat
     4681 gagagcaaaa atacccacaa tcccatcaaa ccgacctgca ccgggagaaa aacaatcccc
     4741 gcgccaatcg ccatcccgat actcataatc acccagccgg tgtcagtact gtcgaatttg
     4801 atcgcttctc gccactcgct ctcactcatt ccagcacgac ccgccggggt cgaagcgtct
     4861 gcaatgacgc ctttattcga tgcaatttcc ataatttctc gctcaatatt ttgtagggct
     4921 tattattttt ttccgagccg catcaaggcg atatgcggtc tacgtttttg caggcgagtt
     4981 attagaataa agaaatgata cgcgcgaggt cagagaaaat cttcacaatc aagactttga
     5041 aataacggag atggataaga attttctact aaattaatcg caagagaata ttcattctct
     5101 atttgcggca gatcacaaaa aaaaggtgca catttgtgca cccaaggatg aaagctgaca
     5161 gcaatgtcag ccgcagacca ctttaatggc cagtcctccg cgtgatgttt cgcggtattt
     5221 atcgttcata tctttgccgg tttcatacat cgtctcgatc actttatcga gtgaaacacg
     5281 cggtgccgag gtgcggcgca tcgccatcca cgcggcgttt actgctttca cggcattaat
     5341 ggcattacgt tcaatgcacg ggatttgtac ctgtccggca accggatcgc aggtcagccc
     5401 aaggttatgc tccatcgcga tttccgccgc attgcatacc tgcgccggac tgccgcccag
     5461 tagttcagtt aaccctgccg ccgccattga acaggccacg ccaatctccc cctgacagcc
     5521 gacctccgcg ccagagatgg aggcgttcat tttatacagc gcgccaatag cccccgctgc
     5581 cagaaaatag cgggcaattg accgctcgtt taccggacga cggaacttat cgtaataagc
     5641 cagcacggcc ggaataatgc cgcacgcacc gttagtcggt gccgttacca cgcgcccgcc
     5701 agctgcgttt tcttcactaa ccgccagcgc gtacatgttg atccagtcga tgacattcat
     5761 cggatcgtta gagatgttat cgctagaaac cagctgacga cgcagcgcta cggcacgacg
     5821 cggcacattg agcggaccag gcagcacgcc ttcagtgttc atcccgcgtt caataccgtc
     5881 atgcatcact tgccagatac gggcaaatcc tgcgtcaatt tccgctttgc tgcgcagcgc
     5941 cagctcgttg tgcatcatca ggccagatat agacaggccg ttgtaatcac acattttcag
     6001 cagttcacct gctgagtgga aatcgtaagg tacggacgtt tcgacatcgt gcgacaggcc
     6061 gaagtgttct tcttcgacaa taaacccgcc gccgacagag taataggttt tacttaatag
     6121 cgcttcctgt cccttccagg cagtgatccg cattccgttc tcatggcgag gcaacatttc
     6181 gggatggaag ataatgttct ttgctacagg aaaatcaaca atatgcgcac cagatgccac
     6241 tggcagccgc ccgctgcgcg ttactaactc tataaatgca gggatctcat caatgacaac
     6301 atcctgcgga ctgtttcctg ccagtcccat gataatggcg acatccgtgg catggccttt
     6361 gcccgtcagt gacaacgacc catacagatc gaccacaata tggctcgtcg cggttaataa
     6421 gccgctactt tccagccgat caataaaact ttttccggca ttcattggcc ccacggtatg
     6481 cgaactggag ggaccaatcc caattttgaa aatatcgaat gcactaatca tatccacacc
     6541 ctcggattgc cgttcagtga agtggagcgg aacgaccttg cgaccgtccc gctcacgagg
     6601 ctttacgcac tacgtactgc gatagcttca atttccagct tcacatcttt cggcaaacgc
     6661 gcaacctgca cacagctccg ggtcgggtag gtcgcctgat gctcatcgaa gaactgctta
     6721 tagacttcat tgatggtggc aaaatcattc agatcggtga tgaacacggt catcttgatg
     6781 atatcgccca cgctcagccc ggcagcaacc acgatcgctt tgacgttttc gaggcttaaa
     6841 cgcgcctgat cttgcacatc agccgggatc tcaccggtct gcgggcaaac cggaatttgc
     6901 ccggaggtga agaccatgct gcctaaatca acgccctgaa cataagggcc gattgcgcct
     6961 ggggcacgtt gcgtttcgat aatctttttc atacatcctc cggcgtcaga gcgcctgggt
     7021 aaaagtacgt gaaataacat cctgttgctg ttcacgggtc agtgcgttga agcgcacggc
     7081 gtagccagag acacggattg tcaggttagg atatttttcc gggtgctcga tggcatccag
     7141 cagcatttcc cgattcatta cgttgacgtt gaggtgttga ccgccttcga catccgcttc
     7201 gtggtggaaa tacccatcca gcaggccgac aaggttggtt ttacgtactg gatcttcttt
     7261 gcccagcgcc gcaggaacga ttgagaaggt gtacgagatc ccatctttgg cgtaggtgaa
     7321 cggcagtttc gccaccgacg tcaatgaggc cacggcacct ttgcggtcac gaccatgcat
     7381 cgggttagcg cccggcgcga acggtgttcc ggcgcgacga ccgtccggcg tattaccggt
     7441 tttctggccg tacaccacgt tagaagtgat agtcagaatc gactgggtag ggacggcgtt
     7501 gcgataggtt ggcagcgctt taattttctt cataaagcgt tcaaccaggt cgcaggcaat
     7561 gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat tcaccgtcga tttcaaagtc
     7621 caccgccagg ccgttttcgt cacggattgg tttcacgcgg gcatatttga tggcagacag
     7681 ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc atagtgcgat agacatcacg
     7741 atcgtgcagc gccatcagcg aagcttcgta gctgtacttg tcgtgcatgt agtgaatgat
     7801 attcagcgcg ctgatgtact gcaccgccag ccagtccatg aagtgatcga ggctgtccat
     7861 cactttgtcg taatccagca cgtcgtccat cagcggtgct gttttcggcc cgacctgaat
     7921 cttcagcttc tcgtccaccc cgccgttaat tgcgtacagc agtgttttcg ccaggttagc
     7981 gcgtgcacca aagaactgca tttgcttacc aatcaccatt gggctgacgc aacaagcaat
     8041 tgcgtaatcg tcgctgttga agtcagtacg catcagatcg tcgttttcat actgcaagga
     8101 agaggtgacg atcgacacct gcgcggcata ttttttgaag gcaatcggta attcttccga
     8161 ccaaagaatg gtcaggttag gttccggtgc cggccccata gtgtgcaggg tgtgcaaata
     8221 gcggaaggag tttttggtca ccagcgtacg accgtccagc cccatcccgc cgatcacttc
     8281 cgtcgcccag attgggtcgc cggagaacag cgaatcaaat tccggcgtac gcaagaagcg
     8341 caccatacgg atcttcataa tgaagtgatc gatcagttcc tgtgcctgct gctcattgag
     8401 tacgcctgct ttaaagtcgc gctcaatgta gatatcgagg aacgacgccg tgcgccccag
     8461 cgacatcgcg ccgccgttct gtgatttcac tgccgccaga taagcgaagt agagccactg
     8521 caccgcttcc tgcgcattct gcgccgggcg agagatatca aagccatatt tcgccgccat
     8581 ttcctgaatc tgcaacagcg catgacgatg ctctgccagc tcctcacgca gacggatggt
     8641 ggcttccaga tcttcgcctt tttccagacg ggactggaga tcggcaaatt gcagttcgcg
     8701 ttcacgcacc agataactga tgccatacag cgctacgcgg cgatagtcac cgataatgcg
     8761 cccacggcca tagccatctg gtaaaccggt cagcacgcca gatttacggc agcgcagcat
     8821 atccggtgag taaacatcaa atacgccctg gttatgggtt ttacgcagat cggtaaacag
     8881 atattcaaat tcactgtcca tttcacggcc ataggcgtgg aatgaacttt taatcatatt
     8941 aatgccaccg aacggatgca gcgcgcgctt taatggcgca tccgtttgca gaccaacaat
     9001 tttttccagc ggctggttaa tatatcccgc atcatgagcg gtaattgtgg tggcaatatt
     9061 ggtatcgaaa tcaaccggcg cgtgggttgc gttttcgata cggatgcctt ccattacttt
     9121 ttcccacaat tccgtggtgg caggcgtcgc ttcggcgagg aaagattcat cgccttcata
     9181 cggtgtatag ttatgttgaa taaaatcgcg gacattaatt tcgtttttcc agtccgtacc
     9241 tttaaagcca agccatgcgt cggcgtacag cttatcgctg gtatcaatat ctatcttcat
     9301 gaaaaataat ctctctacaa tacttcaact aaattatgca aattctgcgg gcgcgttaac
     9361 tttgcctaaa tgaatggcat ccaaagcaat cattttttct tcgttagtcg gaataacggc
     9421 acaaatgaca cgcgcatttt cactggaaac aattcgctct ccaaaagagt tagagcgatt
     9481 attcatttct gtatcaatca ctacgcctaa tacagccaaa tgttccatga ccagacgacg
     9541 aattaaactt gaattctctc ctattccgcc ggtgaatata attccatcca ggcgacgtaa
     9601 tgaagctgcg tgtccggcaa tatgacgggc aattcggtga acaaaggttt taattgccag
     9661 ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga acacgtaaat ccgaagaaag
     9721 accggaaata cctaataatc ccgactcttt attcactacg cgttccaggt cacccaggct
     9781 ctggttggtt tggctggcga cccaggacat cgcaccaaag tcgacatcgc cactgcgggt
     9841 tcccatcatc aagccttcca gcggcgtcat tcccattgaa gtatcaacac tctgaccgtt
     9901 gcgaaccgcg cagattgacg cgccattgcc aagatgcgcc acaaccaggc cggagtcatc
     9961 ttccgccaga ttcagcagag aatgtgcgcg ctgggaaaca tagcggtgcg acgtgccgtg
    10021 gaaaccataa cggcgtacac ctaactcttc ataatatttc cacggcaggc cgtataaata
    10081 agcttccgga gccatcgtct ggtggaaact ggtatcaaat accgccacct gagttacgcc
    10141 cggaaataat tgctgcgccg attcaatacc acttaaattg gcgtaattat gcaggggtgc
    10201 cagtggagaa acgcgacgga tattatcaat gacttcatcg gtaataatgg cggactcggt
    10261 aaaaatactg ccgccgtgag cgatgcggtg gccaattaag gccacactgt catttaaatt
    10321 ccgtttttcc agttcaaatg caattgcctt caatgcacct tcgtagctgt ggtgagccag
    10381 cggtgctggc tctcccccat ttacggataa gaatgcattt tccgagttaa taccgtcggc
    10441 aatacctgac attaatactt cacagtcgct ggcatcgagc acggaaaact taatcgaaga
    10501 cgaaccacag ttaataacca aaacaaccgg aaattcattc atctcttttc tcatcctgag
    10561 ttacggatta aaacagtttg tatacgatgt tcaggatggt cagcagacca atcacggtaa
    10621 caaacacgtt atccagacga ccacggtatt tcgccagaga cggcgcttta cggatggcat
    10681 acatcggcaa caggcacagc agggatgcga taatcggtgc gcccatggct tcaatcaggt
    10741 cgaggatgtt cgggttggcg taggcaacaa cccaggtgga gcccatgatg aagatcatgc
    10801 tgatagtgtt cagtttaccc agcgacactt tagttttgtc gcctttataa ccaaacttca
    10861 ggaccaggcc attcagacct tccagcgttc ccagatagtg accgaagaaa gatttgaaga
    10921 tagccacgag tgcgatgatg gaagccgcat attccagtgt aatcgcgaac gttgttttgg
    10981 taccggtcat ggacgcaaag tggttagcca gataagaaag cactggaata ttctgcgctt
    11041 tggcttccgc catgttggcc ggagacagcg taaataggca gctaaaggca aagaacatca
    11101 ccactgcaac catcagcatg ctggcacgag aaatgatttg ggaacattta cgttcggtga
    11161 agtcgcgacc gaagtctttc tcatactctt cacgtttaga aaccacgaag gaagagacga
    11221 ttggcgagaa attaaaggag aaaaccatga tggaaatccc cagccagacg gtgatcagga
    11281 taccgtcatg accggttaac gacagcgaac cgaggtcaac ctggtcgata actgcagagt
    11341 tccagtaagg gatcagcgac agagaaatca gcaccaggct ggcgataaac ggccatacca
    11401 ggtagctcat cactttaacc atcagatcct taccaaacca gatgacgaaa gccatcagca
    11461 gcagcaggaa cagcgccaca aagccgcgat tcagcggtgc aaagccgagc tggttttccc
    11521 agaacgtcat aaaggtattg gtaatagtaa cgccataaat ccacagcagt gggcaaatcg
    11581 cgaagaagta caggaacgtg ataaccacgc cgccagtttt accaaaatgc tcttccaccg
    11641 tttccgtaat gttgccggaa gggttagagc cagaaagaca tagacgcgcc agcgcccggt
    11701 ggcaataaaa tgcgatgggg tatgccaata ccaacatcag aagaatcggg atcagtccgc
    11761 caaaacctgc gcggataggg aagaacagca ccccggcgcc gattgccgta ccaaacaagc
    11821 ctaacgtcca tgtggtatct gatttacgcc aggacgattg ttttgtctgg ctggatacaa
    11881 tgctatctga agtactcata tcctatcctc aacgaattaa ttaagcgtca acgaaaccgg
    11941 tgatttgaga gacgcgagaa agatcgatat tgccgccgga aataatactg acggtttttc
    12001 tgttttgaat atattggtct aatttaccgc ttaataatgc agcacatgcc agagcgcctg
    12061 cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat catactgttt ctgatttcgt
    12121 cttcgctgac cagcacgatg tcatcgacta attcacgaac gatttcgtaa gttaaattac
    12181 ccgggcggga gacatcacaa ccatccgcca gggtgccggt agttcggtgc gtggttattt
    12241 ctccggagtg gaaagaagcc gccatgccgt gaacgttttc agactgtacg ccaataacac
    12301 gaatggtcgg gttaatagat ttaattgcca ccgcaatacc agcaattaaa ccgccaccac
    12361 caattggcac aatcacgtta tcgacatcat agagatcttc cataatttcc agaccaatcg
    12421 ttccctggcc agcaatcact ttcggatcat cgtaaggtgg gataaaaata cggccttcca
    12481 tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa gttatcacca tgcagaacga
    12541 cttctgcgga gtagtcgcac gttgccgcta ctttggattt tggcgcacct tttggcatca
    12601 ccactttacc gtcgataccc agcatcgcgc aggagagcga aaccccttgc gcatggttgc
    12661 ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc ggtcagtgaa cttaatttat
    12721 taaatgcgcc acgaatttta aatgaacccg tacgctgcat attttcaaac ttcaggaata
    12781 tttcaccttt gcaacgttca ctaaaatagt tggagcgagg catgcctgtt ttataaattc
    12841 gcccagccag tcgttgtttc gcttcaataa tatcatcaat agcaatcggc agatcgtatg
    12901 taatatgcat tataaaacct cttcgcctga attaaatatg taggtaaccg taaccgacac
    12961 ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa acaaataact aaccaacttc
    13021 tattaattgc cttcgtctac acccattata agatgaatac tctttggcta attccaccaa
    13081 aaccgatgct gcttttttaa tacgataatt tttcgaccat accgcggcat attgtgccac
    13141 aggtaatgtt tcttcaaccg gaatagtaat aaattgatta gaaccaaaag gtgacgtcat
    13201 atcacaagga attacagtta agaaatcagc attgagaaca agattataaa ttgtcacgac
    13261 tgagtcggtt ttaacgatgt tttcaatact gatgccattt ctttgtaacg tagtaagcag
    13321 ttcgctgtag taccccatat tagtttgtgg caacacccac tgttcgttct tcaacgactc
    13381 cagcgtggtg gtgccggtgc atgttcggga cttactggct accagcacaa actcggactc
    13441 gaacagcggc tcaacatgta aatcctgaag cttcatttct gcacttaacg taccaatcgc
    13501 aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa gacagttgcg cttcatacat
    13561 agaaacctgc gctttcggga acacctcttt gaacttgttg atcatccctg acataaaagt
    13621 aaaaccaatc aatgaaggaa aaccaaatga gacttccacc accgcctcag aagacatacc
    13681 gcttatctca ttaaccatat ttttcatttc acgggtaatg gattcggaac gggagagtaa
    13741 caattgaccg gcaggtgtta atgttacacc ggtatttttc cgcaccacta attccacacc
    13801 aaaataatct tcaatatcgt taatgatttt actgacggcc ggttgagtta accctaattc
    13861 ttttgcagcc gagccgatag agccacttct aatgacttcc tgaaagacta ccaggtgctg
    13921 cgttttcgga agaagaatag tgctcataac gacctacgtt aattacctca ttgacggcat
    13981 gaagtgtatc aaaatgaaat gaacaggata tgtgcgacca ctcacaaatt aactttcaat
    14041 actttccagg gtatcgtcat tacaattaaa tacctttaaa tatcaacaag ttaaagcata
    14101 aaaatcagca taaaccctga tttttgtcaa aaaaaatgac ggggataacc atattttatg
    14161 gcgataacat cattcgttat gtggttaata caaaaaaggg gctgagacca tatttcaagc
    14221 aattaccgga taattaccag gcaaaatttg agttaaattt aatgagc
//
LOCUS       DAZPLI010000102        14225 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZPLI010000102 DAZPLI010000000
VERSION     DAZPLI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14225
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.103"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            132..1151
                     /locus_tag="ACHUU3_002897"
     CDS             132..1151
                     /locus_tag="ACHUU3_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR2082913.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
     gene            complement(1425..1634)
                     /locus_tag="ACHUU3_002898"
                     /pseudo
     CDS             complement(1425..1634)
                     /locus_tag="ACHUU3_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(1836..2480)
                     /gene="cheZ"
                     /locus_tag="ACHUU3_002899"
     CDS             complement(1836..2480)
                     /gene="cheZ"
                     /locus_tag="ACHUU3_002899"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR2082914.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(2491..2880)
                     /gene="cheY"
                     /locus_tag="ACHUU3_002900"
     CDS             complement(2491..2880)
                     /gene="cheY"
                     /locus_tag="ACHUU3_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR2082915.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(2895..3944)
                     /gene="cheB"
                     /locus_tag="ACHUU3_002901"
     CDS             complement(2895..3944)
                     /gene="cheB"
                     /locus_tag="ACHUU3_002901"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR2082916.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(3947..4807)
                     /gene="cheR"
                     /locus_tag="ACHUU3_002902"
     CDS             complement(3947..4807)
                     /gene="cheR"
                     /locus_tag="ACHUU3_002902"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR2082917.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(4826..6427)
                     /gene="tap"
                     /locus_tag="ACHUU3_002903"
     CDS             complement(4826..6427)
                     /gene="tap"
                     /locus_tag="ACHUU3_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR2082918.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(6473..8134)
                     /gene="tar"
                     /locus_tag="ACHUU3_002904"
     CDS             complement(6473..8134)
                     /gene="tar"
                     /locus_tag="ACHUU3_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR2082919.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(8279..8782)
                     /gene="cheW"
                     /locus_tag="ACHUU3_002905"
     CDS             complement(8279..8782)
                     /gene="cheW"
                     /locus_tag="ACHUU3_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR2082920.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     NGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(8803..10767)
                     /gene="cheA"
                     /locus_tag="ACHUU3_002906"
     CDS             complement(8803..10767)
                     /gene="cheA"
                     /locus_tag="ACHUU3_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR2082921.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(10772..11698)
                     /gene="motB"
                     /locus_tag="ACHUU3_002907"
     CDS             complement(10772..11698)
                     /gene="motB"
                     /locus_tag="ACHUU3_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR2082922.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(11695..12582)
                     /gene="motA"
                     /locus_tag="ACHUU3_002908"
     CDS             complement(11695..12582)
                     /gene="motA"
                     /locus_tag="ACHUU3_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR2082923.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(12708..13286)
                     /gene="flhC"
                     /locus_tag="ACHUU3_002909"
     CDS             complement(12708..13286)
                     /gene="flhC"
                     /locus_tag="ACHUU3_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR2082924.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(13289..13639)
                     /gene="flhD"
                     /locus_tag="ACHUU3_002910"
     CDS             complement(13289..13639)
                     /gene="flhD"
                     /locus_tag="ACHUU3_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR2082925.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         3403 a         3846 c         3479 g         3497 t
ORIGIN      
        1 cacacaatta aacacggagc agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
       61 ctcgcagatt tccggcatca tagtgcccaa tacgggatgc ataaagctcc ctcagactgg
      121 agactccaca aatgaaatat acaccggttg gcgttgatat cgcaaaacat gtcattcaga
      181 ttcacttcat caatgagcac acaggtgaag tggttgataa acagttgcgt agacaggatt
      241 ttctgacgtt cttcggcaac cgtgagccat gcctgattgg tatggaggcc tgtggaggtt
      301 ctcagcactg ggcacgggaa ctgacaaaac ttggtcataa agtccggttg ttgcaggccc
      361 gcttcgttaa ggcattcgtc atgggcaata agaatgatgt gatggatgcc cgggctatct
      421 ggatggcggt tcagcagccg ggtaaagaaa tcgccgtaaa aacagaagaa cagcagtcgg
      481 tactggttct gcaccgtacc cgcatgcaac tggtgaagtt ccggaccgca caaattaatg
      541 ccctgcacgg gacgttactg gagtttggtg aaaccatcca caaaggccgg gcagcgatgg
      601 agcgggagtt ccccgaagca ctggaacgga tgaaagagag actgccaccg tatctcatta
      661 tggttctgga aaaccagtac aaccgactga atgagctgga ctcactgata gaggatattg
      721 aaaaacagct taccagcgtg gcgaggcaga atgaaacctg taagcggttg ctggatattc
      781 ctggcgttgg accacttatt gcgacggcag cggtggccac catgggggaa gcatcagcgt
      841 ttaaatcggg gcgagagttc gccgcatatg ttggtctggt tccaaaacaa actggctccg
      901 gagggaaagt acgtctgctg gggataagca aacgtggtga cacttatctc aggacattat
      961 ttatccacgg tgcaagagcg gtggcattag tagctaaaga gcctggcccg tggataaccg
     1021 aactgaaaaa acgtcgtcca gccagtgtgg caatcgtcgc catggcaaac aagctggcac
     1081 gaacagtatg ggcgataacc gcccatgacc gtaagtatga caggaaccac gtcagtatca
     1141 gaccatatta atcgctgata ccattaaaca atgaactctt aacaaaaggg tgaatgctga
     1201 aaggttgcta tggcggccag agtgatgaca aagacaggta agaccgtgac tcactaaacc
     1261 tgaacagtat tttgggcttg aagtccgccg tgaaaataag gggggagtcg gcgaattaca
     1321 taggggctcg cagcgttacg gctgcaataa agccggatat aaagctgcaa cctacccgtc
     1381 atgtcaaaac aatggatgcc ttgcaaacgg gatgcgttca tataaatact gtgatcaaaa
     1441 tgcagcccag cggagagcat gcccgacaat cgacgggcca gcgacacacc gccaaaccag
     1501 ataacacaca cgcccactaa caaaatcagc aacgaggtca gttcacggga acgcgggatt
     1561 tgcccctctt cccgcgcttt ttctagtcgg tgaggtgtgg gggcttctgt tttgtcgtcg
     1621 ctctcgtcag acacgtcgcc aatcctggaa agagtattaa gcttaaatga tgccagagcg
     1681 caaagcgttc aatggtttga gtaaggggca aaacaggcgg gatttagggc ttttgctgcc
     1741 acacatcaag catagtgtgc gtttgtcgga tgcggcgtca tcgccttatc agaccgcctg
     1801 atatgacgtg gtcacgccac atcaggcaat acaaatcaaa atccaagact atccaacaaa
     1861 tcgtccacct gatcctgact ggctaccacg ccgactttgc tggtatcgac ctgaggtcca
     1921 ttaagcaaac tctggttttc acgttttgga cgcgactcct gttccgggat gttttccaac
     1981 agcaccatca gcaactggcg ttcgatctcc tgaatgacat ccatcatccg cttaatgacc
     2041 agcccggtga gatcctgaaa atcctgcgcc atcatgattt ccagcagttg cgcgttagta
     2101 aagctggtat gcgcgggtac atctgccaga aattgtcgtg tatctgttac cagttcacgg
     2161 gcatcggcaa ggtcaatcgg atcggcaaac cagtcatccc aacgttgggt taacgctttt
     2221 gctgatttct ccatttgatc ctgatgcggt tgtgacgcct caacactgtt cagcgcccgc
     2281 tccgcagcct gggcggtcat ctgcacaaca tagtacaaac gatcgcgcgc atcggggatg
     2341 gcttccgccg cttcggcaat ggcctgatcc agccccagtt cccgcaaact gtcgcgcagc
     2401 atacgcgtca ggctgccgat gcgcgcaatg atatcgccag ctgaatgctc gtcagcaggt
     2461 ttgattgatg gttgcatcat agtcgcatcc tcacatgccc agtttctcaa agattttgtt
     2521 gagtttttcc tccagcgtcg cggcggtaaa tggcttcacc acatagccac tggcccccgc
     2581 ttgcgccgca gcaatgatgt tctctttctt cgcttctgca gtcaccatta acactggcaa
     2641 tgccgacatc gcgccatccg cacgaattgt tttcagcaat tccaggccat ccatattggg
     2701 catgttccag tcggagataa caaatccata accgcctgcc tgcaacttat tgagagcgtc
     2761 gacgccatct tccgcttcct caacattatt gaatcccagc tctttcagca ggttacgcac
     2821 tatgcgtcgc atggtggaaa agtcatccac aaccaaaaat ttaagttctt tatccgccat
     2881 ttcacactcc tgatttaaat acgtatcgcc tgtccggcac taatttttgc caacatttgc
     2941 tggcttacct ggctaagatc gaccacttcg cagacaccac ccatattgat ggcctcgcgc
     3001 ggcatgccga acaccacgca acttgcttcg ttttgcgcaa gggtccatgc ccccgcctga
     3061 cgcatcgcca acattcccgc cgcgccgtcg ttgcccatac cggtcaggat caccccaacc
     3121 gcattacgcc ccgcctgttt ggcgacagaa tggaacaaca catctaccga aggccgatga
     3181 cggttaaccg ccgggccgtc gtgaattttg atttgataat ttgcgccact acgcgccagt
     3241 tccatatgcc gatcgcccgg cgcaatatag gcatgccccg gcaagacacg ttctccgtct
     3301 tcggcttctt taaccccgat ctggcaaagc ttattaagtc tgtcggcaaa agagcgggtg
     3361 aaaccgggcg gcatatgttg ggtaattaac aatgccgggc tggaaagcgg caacggttgc
     3421 agtacgtgac gaattgcctc agttccaccc gttgaagcac caatcgcaat cagtttttca
     3481 gaactcaaca acggacccgc cttcagcgtt gtcggtgctg acaatggctt atgtgctgca
     3541 aggctcgcct ttgctgccgt acgcaccttt tcagcaatca tttcgctata cgccagcatt
     3601 ccttcgcgaa tacccagttg cggtttggtg acaaaatcta ttgcccccag ctccagcgcg
     3661 cgcagcgtga cttctgatcc tttgccggtc aaggaagaaa ccatcacaac gggcattgga
     3721 cgcaaacgca ttaatttttc gaggaaatcc agtccgtcca tccgcggcat ttcaacatcc
     3781 agcgtcagca catcgggatt gaatttctta atcaagtcac gcgcgaccag cggatcaggc
     3841 gcggtcgcca ccatttccat gtcgctatgg ctgttgatga tttctgtcat gatctggcgc
     3901 atcagtgccg aatcatcgac agataacacc ctgattttgc tcatcgttaa tccttactta
     3961 gcgcatacac cgtctgacca cgcagcgtga agcggcgctc aaggtggcta aagttttcag
     4021 agtgacccgc aaacagcaat ccgtcgggtt taaggagcgg aacaaagcgg cgcaaaatct
     4081 cctgctgggt attttgatcg aagtagatca tgacgttacg acagaagatc gcatcaaacg
     4141 gccccggcac ggtgtactgt ttcgccagta gattcagcgg ggcaaaatca acatagttcg
     4201 ccagctcctg acgcacgcgt accagccctt catgcggtcc cgtccctcgc atgaaatacc
     4261 gttgcagttg ctgcggcgtc aggtttttca actcttcatg gcgataaata ccgcttctgg
     4321 ctttttccag cacttcggtg tcgatatcac tggcaaacac tttccagcgt ccgggcgcgg
     4381 tgcccaatgt gtcagccagc gtcatcgcaa tgctgtacgg ctcttcgccg gtcgaagccg
     4441 ccgcgctcca tacgcgatac tcgccagaac ggcgacgtgc gtgatccgcg agcagaggga
     4501 aatggtgtgc ctcacggaaa aatgccgtca gattcgtggt cagcgaattg ataaacgcct
     4561 gccactcacc gctgtgctga ttagattcca gcaagttcag ataatgaccg aaatccgtca
     4621 gtcccagcga acgcaaacga cgaaccagtc ggttgtaaac catgtcgcgt ttatggtcag
     4681 ccagaacgat cccggctcgt tgatagatca attgacttat ccgccgaaaa tgcgcgtcgg
     4741 aaagcgccag gcgctcggtc atctgtaaca ataaagacgt ttgcccacag ggcagagatg
     4801 aagtcatagc gccttctcaa tcacttcagg ataccactgg cgcaatttgt aactgcgccg
     4861 actcatgtcg tgctacttca tgttcttcaa gggtaaatac cgccacacgc gacgaaagat
     4921 ggtcggcctg attcgccagt tgttccgttg ccaccgccgc ttcttctacc agcgaggcgt
     4981 tctgctgagt cacctgatcc atctggctga cagcctgtgc aacctgctca atcccccgct
     5041 gttgttcttc cgacgcagag gcaatttctc ccataatgtc gttcacgcgg gtcaccgaac
     5101 tgacaatatc gatcatggtc gcggcggcgt tattcaccag tttcgaaccc tgctggacac
     5161 gattgactga ctcttcgatc agccctttga tctcttttgc tgcctgcgcg ctacggctgg
     5221 caagattgcg cacttcacct gccactaccg caaaaccacg cccctgctct ccggcgcgag
     5281 ccgcttccac tgccgcattc agggccagaa tattggtctg gaaagcaatt ccgtcgataa
     5341 cgctgataat gtcgccaatt ttttgcgagc tggtggcgat ctcctgcatg gtgtgagtca
     5401 tggtactgac ctgaacaccg cccgcctgcg ccgttgtcgc ggcatttttt gccagttccg
     5461 acgcctgtcg tgcgttatcg gcgttttgcc ctaccgtggc ggttaattgc tccatactgg
     5521 cggccgtttg tgccagcgat gccgcctgct gttcggtacg acttgagaga tcgttattgc
     5581 ctgcgacaat ctccgcgata ccaatgtgca tctcctggct tcccttacgc acatcactta
     5641 ccgtcccacg caaagcctgc tgcatggtct tcagactggc aaaaatggcg gtgatctcat
     5701 tacgaccata taccgcaatc ggacgcgcca gattacccgc agcaatgctg tcaaaatggc
     5761 taccgataat ggccagtggt tgaacaatca tcttgcgcgt ccaccacagc gcactgctga
     5821 tgtagatcgc cgcaacaata atcatgctga taaacaccag tgtcgaaatc tgatagttac
     5881 gctggctttg cgcactggcg gcttccagca catggttgat ctccagctgc caggcctcaa
     5941 agttaacgtc aaacgcattc tgtgatccct gcaccggcgc agtgaggaaa tccgaaagtt
     6001 gattactttc aagccaggtc gcctgatgtt cgagatcgtt gtgccagcgg gcaaaacttt
     6061 tctccgtctc tttttgcaat gccctgacgt gctcgttgcc cgcagtcatc gccataaaac
     6121 ttttaaacag cgtggtggat tgcgtcagac tggcgcgcgc cgtcgtcatc aacgttttaa
     6181 tgtcatccgc cggatagcta agtgccgtca gagtgcccgc tttgttcagc gcggtactgg
     6241 cctgtaacat taccgcccga gtttgcgcta atgccgcacg ttgctgattg ctctgctcga
     6301 cctgattcag tcgttgcaaa tcgtcgcgaa atgcccaaaa agacatgccg ttactgccaa
     6361 tctgcaagat cccgcagaga atcaaaatta aaaacagcgt ggtcgaaatt cgaatacgat
     6421 taaacatcaa cgctccccat caggcggcaa tgaccgcgtt agtaaatact cgtcaaaatg
     6481 tttcccagtt tggatcttgt tcagcaattc gcagtcgtgg ctgtgccggt ggttgctcac
     6541 tggcaggacg ggatggtgtt tgcggtttat tggtgagtgg gctggctgcc agacggaacg
     6601 cggaaaccgc ttgcgttaaa cgactcgcct gttcttccag cgcagcggcg gcggcagctg
     6661 attcctgcac cagcgatgcg ttctgttgcg tgacgcgatc catttccgaa accgccaatg
     6721 cgacttgatc gatgccacgg ctctgttcat ccgatgccga tgcaatctcg cccataatgt
     6781 cagtcacgcg agtgacagca ttgacgatat tgttcattgt ttccccggcg ctttcgacca
     6841 gcaccgaacc ggtatcaacg cgtgcgacgg agtcttcaat gagggctttg atctcttttg
     6901 ccgcctgggc gctgcgactg gcaagattac gcacttcacc cgccaccacg gcaaaaccac
     6961 ggccctgttc acccgcacgc gcggcttcaa ccgcggcatt cagcgcgagg atattagtct
     7021 ggaaggcaat accgtcgata acgctgataa tgtcggcaat tttcttcgaa ctatcggcga
     7081 tctcatgcat cgttttcact acgccatcca ccactttgcc gccgtgctgg gcggtgtcgg
     7141 aggcactttg cgccagttgc gaggcctggc gggcgttatc ggcgttttgc ttcactgtcg
     7201 cggtgagctg ctccatgctg gcggcagttt cttccagcgc ggatgcctgc tgttcagtac
     7261 gggaggaaag atcggtgttg cccgccgcaa tttcacgggt accggcatag atggcatctg
     7321 aaccttcgcg gacatgagtg acggtgtcag tcaaagagcg ttgcatatgt gaaacgctct
     7381 gcgccaggtc gcccatttca ctgcgcccgt caatggtcag ggtattcgct aggttaccac
     7441 cggcgatttc gcgaatgtga gcaataattt ttgccagcgg agtaagcaac atacggcgaa
     7501 tgccgtacca cgccaccagc agaatcaata ccaccaccag cgcgataacc gccagttgcc
     7561 actgggcaaa tcggtaatca tctgcgttgt cagtgacgat atcgcgatac agtttttcac
     7621 tgctgagggc gtactgagca aacgcttcgc ccattgcatt ttgcattccc tgggttggct
     7681 gagcgaaata agctccagta ttgccataat ctagataatc aatcagttca gttaacgctg
     7741 tgtaatagtt tttatatttt tcatcaatat tacgactggt agcgaccatt tcaggtaacg
     7801 gtgccatgct tttgaatttt ttataatgcg tcgctgcctg cgccaatgtt ttcctggcgc
     7861 tatcgagcaa ttcaactttg gcgttacttt gttgattgga ggaatccatc atcatccgca
     7921 ccgctgaacg actcaggtta atgcgcgttt gcagcattaa atcccaggtt gacgtcagct
     7981 cgccctgctg ttcccgcaat tgattggaaa ccacaaagct cttctggcta tggtgaaggg
     8041 aagaaaaaaa cagactgccg gaaataagct gtaacagtgc gaataccccc agcaccatta
     8101 ccaacagcgt gactacgcgg atacggttaa tcataaggca ccttcctgaa aacaagttga
     8161 tctcgttatc ggcaagaagg ggggaaactt tattgctgat gccacccgcc gcgaaattga
     8221 aataaaaaac ccgatgcgca gatcatcggg ttcatttcaa ttgaggaaat cgggagaatt
     8281 acgccacttc tgacgccgcg ctatctaaca gcgccatctc ttcgctgttc agcagttttt
     8341 cgatgttcac cagaatcaac atacggtcgc ccagtgcgcc cagtccagtg agatattctg
     8401 ttgaaagcgt cacggcaaac tctggtgccg gacgaatttg ctccgccgtc aatgaaagca
     8461 cgtctgaaac gccgttaacc acgatgccga ccacccgctg tccgagattc aggacgataa
     8521 ctaccgtgtt gtcgttatag tccacatcca cctggctgaa cttaattcgt aagtcaacaa
     8581 tcggcacaat aacgccgcgc agattcgtga cgcctttgat aaacgctggc gtgttcgcaa
     8641 tccgtgttac ctgatcgtag ccacggatct cctgcacttt caggatatca ataccgtact
     8701 cttcatcacc aagggtaaat accagaaatt cctggcctga cggctcgctg gccagctttg
     8761 ttacattcgt cataccggtc atattgttac ctttttactc attcaggcgg cggtgttcgc
     8821 catacgttgt tcgcggttta tcgcctgcaa ggcggagaca tcaacaatca gtgccacgct
     8881 gccgtcgcca agaatggtcg cagcagaaat gccggggact ttgcgatagt tactttcaag
     8941 gtttttaacc acaacctggt gttgaccaat taattgatcc accagcaagg cgtagcggcg
     9001 accgccactt tgtaagatca caacaattcc ctgggtggct tcggttttcg cgcccgcgac
     9061 gttgaacact ttccacagtt cgacgatggg cagatattca ccccgcactt ccagcacccg
     9121 ctcgccgccg gccagtggat ggagatcggc ttcacggggt tgcagtgatt ccataacagc
     9181 attcagcggc agaatgaaaa cttcatcccc aacgcgtacg gacatgccgt cgaggatggc
     9241 cagcgtcagc ggcagtaaaa tgcggatcgt agtgccagtc ccctgcttcg actggatttc
     9301 gacatgaccg cccatctcct ggatattacg tttaacgacg tccatgccga cgccgcgccc
     9361 ggagacgtcg gtgacctgct ctgccatgga gaagcctggt gcaaatatca gcatcgcgac
     9421 ttcgtcgtcg ctcatgtttt cgctgacagt caaaccttgc gaggccgctt ttgccagaat
     9481 tcgctcacgg tttagccccg ccccatcgtc ggtcacttca atgcaaatgt tgccgccctg
     9541 atgttcggca gacagaatta aatttccgac gctgttttta cctgcggcga gccgtttttc
     9601 tggcagttca ataccgtgat cgaggctatt gcgtaccagg tgggtcagcg ggtcgataat
     9661 gcgttctatc aggcttttgt cgagttcagt agaactgccc accagcgtca gttctacctg
     9721 cttgccgagt tttcccgcca gatcacgcac cagccgggga tagcgactaa aaacatattc
     9781 catcggcatc atgcgaatcg acatcactga ttcctgcaaa tcacgggcgt tacgttgtaa
     9841 ctgccccatg ctggttatca aatcaccatg attaaccggg tccagttcgc tggaacgctg
     9901 ggcaagcatg gactgggtga taaccagctc gccgacgagg ttaattaatt gatcaacctt
     9961 ttctaccgct acacggatgc tggtggattc attgctgcgc gtcgtttttt cccgctccac
    10021 gcggccggtt ggcgcttgtt cggctgccag tttaagcact ggtggggtgg atatttttgg
    10081 cgagacttct actgtttcaa aggtaatctg atcggcttca atcacaaaac agagtaccgc
    10141 tgtgatgtca tcttcggcga tgtcgcccgg taatattgcc gagagcgaat ccgccccttt
    10201 caccacgtca gttaacgttg tcagatgtcc cagttcttct tccagcaggt cgacttcccc
    10261 ggccttcagg cgcgaaagga taattcgtcg cggcgactga ctgcgactct gctcatcttg
    10321 cggttcactt ttggcaacca cacttaatcg ggtcactgcg gatggcgttt cgcctttcgc
    10381 ttctaatgcc agttgacgca aggcctggca gatataatcg aagctggcgg catccggctc
    10441 ttgcgactgt ttataagcgt cgagctgttc ttgcatgatg tccttcgttt ccaaaaacag
    10501 attgataatg tcggtgttga gttgcatctc acctcgtctg gcttcatcga gcaggttttc
    10561 catcagatgc gtggtttcct gcaaaacgct gaagccaaaa gttcctgccc ctcctttgat
    10621 cgagtgggca gcccgaaaga tggcattcaa ttgttcggca tctggcgctt ccggctgcaa
    10681 aaccagcaga tgctgctcca tgtcagccaa cagttcgtcc gcttcatcaa aaaatgtctg
    10741 ataaaaatcg cttatatcca tgctcacgct gtcacctcgg ttcggctgat ggcattgtgg
    10801 gaacactgac ctgtggtgca acctcaggtt tttccagggc gcttactggc tcattctggc
    10861 tttcggcgtt ttcatgcaaa atggcctgtt cggcttgttt gttcagtacc agcaggctga
    10921 tgcgacggtt gacggcatca tcaggtccgc gatcgcttaa gcgcatcgtt gccgccatgc
    10981 cgacgacacg taacactttg ccgctatcca accctccgac catcagttcg cggcgggatg
    11041 cattggcccg atcggcagaa agctcccagt tgctatatcc tttctcaccg ctggcgtagg
    11101 ggaaatcatc ggtatgacct gaaaggctaa tacggttggg aataccgttc agtacaggcg
    11161 caatggcacg cagaatgtcg cgcatatagg gttcgacatc ggcactgccg gttctaaaca
    11221 tcgggcgatt ctggctatcg atgatctgaa tacgtagacc ttcctggacc agatcgattt
    11281 tgagatgggg acgtaacgcc cgcagtttcg gatcggactc tatcaactgg tcgagatcac
    11341 cccgcaattt ccgcaatcga ctttgctcca tgcgtttttt cagatcttcg atgttcggct
    11401 gcttattcac ttccccctgg ctttgggtgt aatcatcacc accgccggga attgggcttt
    11461 cactattaga aatgcgatcg ccgcccgtaa ccgcagtcgc cagtggagtc cggaagtact
    11521 ccgcaatctg aatcagctct tttgggctgg agatggagat cagccacatc accagaaaaa
    11581 aggccatcat cgcagtcata aagtcggcat aagcaatctt ccacgatcca tgtgctgccc
    11641 cgtggctttt ggctttgcgt cgtttgacga caataatcgg atgcgcttga ttcttcatgc
    11701 ttcctcggtt gtcgtctgtt gttgcggatt tttcaccgca cgcacatgct cttccagttc
    11761 aataaacgac ggacgttcgc tggaatagag cgttttgcga ccaaactcaa cggcgatagg
    11821 cggtgcgtaa ccgttcagat tagaaagcag agtgactttg acgcactgca tcattttgct
    11881 ggtttcggcg cttttctgac gtaaaacact cgctaatggg gaaataaatc cgtaagccaa
    11941 taaaatgccg aggaaagtcc ccaccatcgc atgtgcgata agcgcaccca gctcggcggc
    12001 aggacgatcg gctgaaccta acgcgtgaac gccccccatt acagccgcga caataccaaa
    12061 cgccggaagt gagtccccga ccagcgccag actgttcgcc gggacttctg cctcgctttc
    12121 gtgcgtctca atctcttcat ccatcagggc ttcgatttcg aaggtgttca tatgaccgct
    12181 gataatcaga cgcagataat cgacgataaa atcaagcatg acgctatccg cgaggatgcg
    12241 tgggtagctg gcgaagatct cgctctcacg gggattttca atatcacgtt ccagcgaaaa
    12301 catccccatc tgccgcgatt tcgccatcaa ccgataaagc agagccagca gatccatata
    12361 cattgctttg gtgtatttgg agcgacgaaa cagcaacggc agcgccttca gcgtgccttt
    12421 aatcgctttg ccattattgc cgacgataaa cgacccaatc cctgcaccgg caataatcac
    12481 cagttcagcg ggttgataga gtgctccaag gcttccaccg gtcatcaaat aaccgccgaa
    12541 aactgtaccg agaacaacca ggtaacctaa taagataagc acgacatcat ccttccactg
    12601 ttgaccatga caggatgttc agtcgtcagg cgttaacgcg cgattggggc aaaaaaagca
    12661 gcggtacgtc gttaccgctg ctggaatgtt gcgcctcacc gtatcagtta aacagcctgt
    12721 actctctgtt catccagcag ttgtgggata atatcggcag gattctggga aagtttacgt
    12781 ctttttactg cccgggatgg cggttgacat aagctgcagg caaagctgcc aacaggctgg
    12841 tgagcgtggg taataaaatt gccgccgcag cagttgcagc tggaaagttg cagtaatcca
    12901 ctttcaacaa accgcaccaa tgtccaggca cgggttaatg ccagcagtgg tccttcttct
    12961 gcttgtgggc actgttcaag gtataaacgg taggctttga tcaccgcatc gacgccatta
    13021 cacaaaccgg ttttcagtaa aaactgccat gcattacaga acatcgaagc atgaacgttt
    13081 tgttcccagg tcataaacca gtcggttgag aatggcagca tgcctttcgg cggtgggctt
    13141 ccgcgcagtt ctttataaag ttttatcagg cgtccgcgac ttaactgtgt ttcgctttcc
    13201 agcatctgca aacgagcgcc cagggtaatc aattccattg ccagctgaat atcccgcgct
    13261 tcctgaacaa tgcttttttc actcatgatc aggccctttt cttgcgcagc gcttcttcag
    13321 gctgattaac atcattcagc aagcgtgttg agagcatgat gccggtatga atttgctgga
    13381 gatcgtcaac gcgggaatct tgcgtcaact gagtaatcgt ctggtggctg tcaaaacgga
    13441 agtgacaaac cagttgattg gtttctgcca gcttaaccat ttgcggaaga gtcagtgccg
    13501 ctaacgttgt cgccatttct tcatttatgc cgagacgaaa catagcggac gctttgtcct
    13561 gaacaatcaa acgctgtgca agtagtaaat atgacaagtt gatgtcataa atgtgtttca
    13621 gcaactcgga ggtatgcatt attcccaccc agaataacca actttatttt tatgcggttt
    13681 caccgcaccc cgtgatgtcg ccgggaagcc ccggtaaaaa ataattagca ttagaatagt
    13741 tgcgataagc tgcaataagc agaaccacct ttttggttta atatgtcctt acaaatagaa
    13801 atgggtcttt acacttatct aagatttttc ctaaatcgac gcaactgtac tcgtcactac
    13861 acgcacatac aacggagggg ggctgcgatt ttcaataatg tgtgatgcag atcacacaaa
    13921 acactcaatt acttaacata aatgtataag tcatactttt gttttgggtg tatttcaata
    13981 tgttaaaaag tttttcgcta cgctagcaag caaaaatgaa acaggaataa tcgaaatggg
    14041 atgttgcgca cagttaaaat aaatcaccgt aaataatcat ctgctataaa taatcacttt
    14101 catgcaatac cagataagct atttttaaac agacacttac cgcacaacaa actaattaaa
    14161 tacaaagaaa ggcaacaatc aacttaatgt taatgaaatg ttttacatat attaaccact
    14221 tgttt
//
LOCUS       DAZPLI010000103        13989 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZPLI010000103 DAZPLI010000000
VERSION     DAZPLI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13989
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.104"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..1765)
                     /locus_tag="ACHUU3_002911"
     CDS             complement(<1..1765)
                     /locus_tag="ACHUU3_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR2082926.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTMVVNTVASENPD
                     EAGRYSMDVEHGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVRPEA
                     LRRFEQMVEEVSRNASAVAQNTAAAKKSASEAATHATDAAASARAASTSAGQAASSAQ
                     SASSSAGTASTKAREAAKSAAAESSKSAAATSASAAKTSETNAAASQKSAATSASTAT
                     TKASEAATSARGAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKTSETNAKSS
                     ETAAGQSASAAAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSASTATTKAG
                     KATEQATAAARSASAAKTSETNAKTSADNAASSKAAAASSASSAASSASSASASKDEA
                     TRQASAAKGSATTATTKASEAAGSATAASQSKVAAESAATRAETAAKRAEDIASAVAL
                     EDASTTKKGIVQLSSATNSTSESLAATPKAVKAVMGETNKKAPLNSPALTGTPTTPTA
                     RQGTNNTQIASTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQ
                     PKDATLTALAGLTTAAGKFPYF"
     gene            complement(1917..2516)
                     /locus_tag="ACHUU3_002912"
     CDS             complement(1917..2516)
                     /locus_tag="ACHUU3_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR2082927.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMVGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYEGSGSGDWRTDAFIVGIGYRI"
     gene            complement(2584..6035)
                     /locus_tag="ACHUU3_002913"
                     /pseudo
     CDS             complement(2584..6035)
                     /locus_tag="ACHUU3_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515772.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(6379..7059)
                     /locus_tag="ACHUU3_002914"
     CDS             complement(6379..7059)
                     /locus_tag="ACHUU3_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR2082928.1"
                     /translation="MATTNAFSLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGYDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            complement(6957..7700)
                     /locus_tag="ACHUU3_002915"
     CDS             complement(6957..7700)
                     /locus_tag="ACHUU3_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR2082929.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            complement(7711..8409)
                     /locus_tag="ACHUU3_002916"
     CDS             complement(7711..8409)
                     /locus_tag="ACHUU3_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR2082930.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            complement(8409..8738)
                     /locus_tag="ACHUU3_002917"
     CDS             complement(8409..8738)
                     /locus_tag="ACHUU3_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2082931.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(8735..11308)
                     /locus_tag="ACHUU3_002918"
     CDS             complement(8735..11308)
                     /locus_tag="ACHUU3_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR2082932.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLADAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTSSFRANLQGAWDDRENARLGLAAATLQSDMEKAGELAARD
                     RAERESSQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMASAK
                     KDYESTQKKPSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDLWK
                     AENQYVVLKEAATKRQLSEQEKSLLAHEKETLEYKRQLAELGDKIEHQKRLNELAQQA
                     ARFEQQQSAKQAAISAKARGLTDRQAQRESEEQRLREVYGDNPAALAKATSALKNTWS
                     AEEQLRGSWMAGMKSGWGEWAESATDSFSQVKNAATQTFDGIAQNMAAMLIGSEQNWR
                     GFTRSVLSMLTEIFLKQAMVGIVGSIGSAIGGAFGGGASASTGTAIQAAAANFHFATG
                     GFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMR
                     RAEGINFNQNNHVVIQNDGPNGRAGPQLMKAVYEMARKGAQDELRLQLRDGGMLSGSG
                     G"
     gene            complement(11289..11702)
                     /locus_tag="ACHUU3_002919"
     CDS             complement(11289..11702)
                     /locus_tag="ACHUU3_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR2082933.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            complement(11729..12160)
                     /locus_tag="ACHUU3_002920"
     CDS             complement(11729..12160)
                     /locus_tag="ACHUU3_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR2082934.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            complement(12174..12926)
                     /locus_tag="ACHUU3_002921"
     CDS             complement(12174..12926)
                     /locus_tag="ACHUU3_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2082935.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKADAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VSAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(12934..13329)
                     /locus_tag="ACHUU3_002922"
     CDS             complement(12934..13329)
                     /locus_tag="ACHUU3_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR2082936.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(13326..13901)
                     /locus_tag="ACHUU3_002923"
     CDS             complement(13326..13901)
                     /locus_tag="ACHUU3_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2082937.1"
                     /translation="MAIKGLDQAIDNLNRVRKNAIPAASAMTINRVATTAINQSSSQV
                     ARETRVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEEGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPKELAYALKQQLRIAIKR"
     gene            complement(13916..>13989)
                     /locus_tag="ACHUU3_002924"
     CDS             complement(13916..>13989)
                     /locus_tag="ACHUU3_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR2082938.1"
                     /translation="PDDGGSCYLWLNRGQPPAVNRRR"
BASE COUNT         2804 a         4375 c         3483 g         3327 t
ORIGIN      
        1 taaaatacgg aaacttgcct gccgcagtag taagtccggc cagcgccgtc agggtggcat
       61 ctttcggttg cttacccgca agcgcgttag tcatggtggt cgcaaaattc gggtcgttgc
      121 ccagcgccgc agccagctcg ttcagcgtgt tcagtgcatc aggtgacgaa tctacaaggg
      181 cggcaatcgc agccataacg aaagccgtgc ttgcgatttg ggtattattc gttccctgtc
      241 gcgcagttgg tgttgttggc gttccggtca gtgcaggact atttaagggc gctttcttgt
      301 tcgtttcacc cattaccgcc ttaaccgctt ttggcgttgc cgccagtgac tcggaagtgc
      361 tgttggtcgc actgctgagc tgtactatcc cctttttcgt cgtgctcgca tcctccagcg
      421 ccacggcgga tgcaatatcc tctgcccgtt ttgctgctgt ctcggcgcgc gttgccgcgg
      481 attcagcagc aactttgctc tgagatgcag ccgtcgcact gcctgccgcc tctgatgctt
      541 tcgttgttgc tgtcgtggca ctacctttcg ctgctgacgc ttgtctggtc gcctcatctt
      601 ttgaagcaga cgcagatgat gccgatgacg ccgctgaact ggctgacgat gcggctgctg
      661 ccttagagga agcagcattg tctgctgaag tctttgcatt tgtttcagag gtttttgctg
      721 cagaagcaga cctcgctgct gcagtggctt gctcagtggc tttgccagcc ttcgttgtgg
      781 ctgttgaagc ggatgatgcg gcgctttctg ccgattttcc ggcggcggtg gcactggctg
      841 aggcctgccc ggcacttgtt gacgcggcac tggcagataa tgcagccgct gtttttgaac
      901 ctgccgcagc tgaggcgctc tgtcccgctg ctgtttcaga agacttagcg ttcgtctcgg
      961 acgtttttgc cgccttcgcg gaatttcctg ccgccgttgc cgaggaagct gcactactgg
     1021 cacttgatga tgcattcgtt tctgaagatt tcgctgcctc ttttgaggcc gccgcacccc
     1081 gtgccgaggt ggcagcttct gacgccttcg tggtcgctgt ggatgcagaa gtggctgccg
     1141 atttttgtga tgctgcggca ttcgtttctg acgttttcgc ggcactggcg ctggtagctg
     1201 ccgcgctttt tgatgactct gcagcagcac tttttgctgc ttcacgggcc tttgtcgatg
     1261 ccgttcctgc gctggaagac gctgactgag ccgacgacgc ggcctgtccg gctgacgtgc
     1321 tggcggcacg tgctgaggct gcagcatcgg ttgcatgagt tgccgcctcg ctggctgatt
     1381 ttttcgcggc tgccgtattc tgtgcaaccg cggaggcgtt acgtgacacc tcttccacca
     1441 tctgctcaaa gcggcgcagt gcctccggtc ggacatcatc ctccgtcatg gcaccgagaa
     1501 aatcattcag cgtacctggt ctggaacctt catagacggt aatggtcccg gcatgtgaag
     1561 gcggaaaacc ttcaaccagc agggtgacgc tgtactgacc atgctcaaca tccatgctgt
     1621 aacgtccggc ttcatccgga ttttcagagg ccaccgtgtt caccaccatc gtgctgctgg
     1681 ttcgtctggc cttcagcaca atggtgcagt tctgtactgg ttttcctgtg ccatctttaa
     1741 gcacgccaga aatttttact gtcatacttt tccaccaata aaaaaagccc gcagcagtga
     1801 cgccacgggc ttcaggacag tgtaacttta cgtttcctca aacgcagttc accccataag
     1861 gtggatgaac ctgcgtatca taacaatatt tacagaagat aaatcggcgt ctgttgtcag
     1921 atacggtatc cgataccaac aataaatgca tccgttcgcc agtcaccact accggaacct
     1981 tcataagcaa ggtcaatggt cacggattcg gtcgggttaa actgcacgcc agccccccac
     2041 gccagagacg tgttgctgtg gcgaccgtca tcacttccgg tcagcacatc gtgcgttttc
     2101 cccttgttgt cagttacgcg gagataatcc ccggagaaag tcgacacacg gctgtaagcc
     2161 acacccacca tcgcatacgc gctgaaccat tcattcacgc gtacagacgg ccccgccatc
     2221 acgctgaacc agcggttacg cacggaatct tcatgccagc gggtatcgct gtagtgcgtt
     2281 ttttgctcat cctcagcatt ggcataactg aaggacgtaa tcagccccag cgcgtccgta
     2341 aactcataac ggtatttcac gttaatcccg ttcagattat cgctgccggg agcgttcgta
     2401 cgggcatgaa gataccccgc gctcagtgtg gactgatgtt cagacgccca tgcaggcgca
     2461 ccggatacgg acagacagat ggctgcggac aaaatggctg cacaaacttt acgcataatt
     2521 acctctcgct tttctgcaat aaaaaaggcg ccatttctgg cgcccgtata tgggttataa
     2581 aattcagctg atactgatgc ctgcggtggc tttcttcatc accacaacca gcaaatcgct
     2641 gatacttgct gtgggatacc agttattcac cagccatgct gacaccgaaa actccagcgt
     2701 catgtggccg cgaccagctg gcatatcaat aacaccactg taaatcagcg tattatccag
     2761 cgcggtacgg ttataaattt cagcaccgtt tttccgtact atcaggcggc atgacgaata
     2821 aatatcgtta ttctcccgct catgtttagc gccgctgaat gccaccgccg gaataacaat
     2881 ttgcctgtca aaaggctgat cgtcataaat cctgacggta atggtccctg atggccaccg
     2941 ctccggtgcc cgggagtccc gcggaaaagc cttacccact gttttgacta tatcgccttc
     3001 aatctggttg gctgacagtt tccccttaat ctgacagttc tcattaattg tgacattgtt
     3061 gagcgtcccg gcgttcgcat tcacactgcc actgatatct gcatttttag cggtcagttt
     3121 tccgtccggt gtcagggaaa atgccggagg attaccgccg ctggtaatgg tgggagccgt
     3181 caggcgtttc aggaacacgt cgttcatgaa tatctgatcg ccctgaccaa caaacatcgg
     3241 ctttgtgttg ccattcgcag gattaatcat cgcaatcctg tctgccgcca gcagcacctg
     3301 actctgcatt cctgctggcg tattctcaat accggcaccg atacccgcaa tataaaggcg
     3361 tccgtccttc atctgttgca gcttcacagc ccacatgctg ttcagattat tatttgtatc
     3421 aacctgaacc ttctgtatct gctggattgc cgcactctgg tctttcagtt tcttattgac
     3481 agtctgcgtg atttcattgc tgacatccgt aatggacgtc ctgatttcag ccaggtcagg
     3541 cgcaagctga ccgttatcaa tctgcgtcca cagctcctga gccagatgtg ttttccctat
     3601 ctctcctttg aaaaaatcca agtaaccttc cgcatcatcg ctcgcccgac cgacagcctc
     3661 cacgaatgcc gatttgccaa cggtgttcac actgcgaacg taaaaataat aatcatggcc
     3721 cggtttgata ttgatactgg cggctatcca gtacagcgcc gtgccaagat agcgggcgct
     3781 ggtttcaacc tgcctgatat ccgcaatccg cttttccgag aaccagaact caaactgtac
     3841 cgtcgggtca taaacggcaa gatgcggcgt tgcggttatc tgaaaatagc ccggcgtcag
     3901 ctcaatccgc gacggtgctg ccggtgcggc aatccggaag gtggtggtgg caggttcacc
     3961 ctgctggcca tagctgttta tcgcccgcac cgtcagggtg tattccccga gcggcaggcc
     4021 gctgaaacgg tgctccgtgt ctgcggtgat ggcggtggtc accagtctgg catccgttcc
     4081 cttaccactg gtcaggcgca gactgaagcg cacacccttc accacccgcg gcgtgtccca
     4141 tttcgcctgc gccagatact ggccgtcagg tgctgcactg ccggtgggat gacgctgttc
     4201 agggaacctg actgcggctc aaagcgggca ccgttatcca cgatggcttc tttttccagt
     4261 acgtgctgca ccgccgtgat ggcaaaggtg ccgtccgtgt tttcccggac ggagacacag
     4321 cggaacagga gacggcgcag tgacggcagg gagagtcccc acaccccgta tgtctccaca
     4381 ccatcaggca gggtactgac ctgtatccgg tccggcgcgg ggtgttcggt gatgtccaca
     4441 ctcaccggct taccgctgcc gttaatcagg ttcaccgtgg cggcaccggt ctccggaagt
     4501 gtcacttcac ggtccagcgt cagagtgcgg gtggcagcat caatggacag gacacgtccg
     4561 ccggtcaggg tcccggcata gtcgttatca cagatttcaa tgatgtcacc gggtgtgtgc
     4621 cgcagcccct gagacccgag cgtgaaatcc accgtctgcg tttccagcag ttcggtcttt
     4681 atcacccaca gtccggcacg gtgggcctga ccgcggctgg tacagccgaa cgcgtccatc
     4741 ttcagcaggt tgcgtccgta gcgcagtatg gcttccgggt cttccaccag ttccgtggag
     4801 gtctgccagc cgttctgcgg gtcggtataa ttcacctcca ccgccgtgtg ccggtccttc
     4861 agggcactga agctgtagcg gaaacccacg ccgttatcat ccaccaccac atcgctgttg
     4921 gtgtacggcc acaccacatc cgacgggcgg tcctgaacaa acgtcagcgt ctggccgttc
     4981 cataccggca tacagcgcat cgccgagcag aaatcactga gcacgtccca tgccttacgc
     5041 tgttgtgcca ggtacgcatt gaaggtcatc cgcggctcgg tctccccgaa actatccggg
     5101 accgtctggt cgcagtactg cccgatggca tacagcgccc acttgtccac atccgccgcc
     5161 cccagacgtt ttcccatgcc gtagcgcggg tgagtcagca tgtcccacag acaccaggcc
     5221 ggattgttgc tgtatgccgg tttcaggcta ccgtcccaga taccactgta cgtgcgtttt
     5281 tccgggtcat agtttgacgg cacctggatg atgcgaccgc ggatatggta gttcaccgtc
     5341 atctgctggc cgccgaactg ctccgcatcc acctgcagcc ccacaatggc cgtgttcggg
     5401 tagcactgtt tcacatcgat gatttcggtg tatgacgacc acagcgtctt attctgcagc
     5461 tggtccgtgg tgctgtccgc tgtctcccgg accatccgga tgttaaaagg acggggaggc
     5521 agattatcca gaatcaccga cgccagaaac tgcgaggtgg tcttgccgtt aatggtgaca
     5581 tccttttctg tcacccagtt accgttacgc tgtaactgaa tcagcaggcg gacggatgtc
     5641 gggtttcggt caccctttga cgtggtctgc accagtgact gcaccccgaa ggtaacccgc
     5701 aggcggtcaa tgttcgcgga cgtaatggtg cgcgtcaccg gttttgcctt cgtcacttcc
     5761 acgcccagtc cggtttcagc accggaggac tcaaagcctt ccggtggtgt ctgctcctgc
     5821 tccccggcgc gtcagaccgc cgtcacaccg tgtatcacgg gattaccgtc cgtgtccgtc
     5881 agcggggttt tgttcaccag gatactctgc agtcccttca ccggaccttc tatcagtccc
     5941 tcaccaatcg catcaatcac gctcatcatc tgcgtggatt tgagattatc cttcgcctca
     6001 cgaggcgtgt gtgccttacc gccacctttt cccatacagc cttcccctga ataaattaac
     6061 cgccacttgc cattccgtac agaagtcgga tatccttcgc ccgaaaagca tgaaacacat
     6121 ttctgccatg ctaaagagaa accccggtat cagcagatac cggggttttc tttcatgccc
     6181 accgataatc ctgttggtta aaaccggtaa tggcataaaa attctgaata tcttcacatt
     6241 ttcacaaact gactgtggcg cgtataattt ctctgcgtta atttttttgt cgtgatataa
     6301 gaataattcc ttacacttaa tcttcgtaac tctcccgcag ttcctgtccg cgatcactgc
     6361 gggatttttt tattcttttt acccctgccg cccgataacc acgacctttc cgcccccgcc
     6421 ttcatcacgg gtgctgatgt cctgggatat acggcgggag ccaaccagca tttccccgta
     6481 aggcaccggc atcgggttcc cctgggcaat catgttatcc agtgaggaaa agtacgtgtt
     6541 ctgtctgccg ttatccgttg cgcggtaatc cggtgttttt gccttcgggg ccagcatctg
     6601 ggccacaccg cccagaatca tgctggctcc aagtgaaaac agcatcgtgg tggcagaaaa
     6661 accaccggct gccagggctg aaccccataa cgccattgat gccccggcag tgaagaaaga
     6721 gcccacgatg gctgccgccc ccagcacaat ctgcagtcca ccctttccgg ccccggccag
     6781 tcgcggcaca atgtggatga ccgttccctc acccagctgt tcgtgaagac gggcgtacac
     6841 cgcctccggt gccgtgtcat aaccggcaat acgtatctgg taccagcctt cgttcatctg
     6901 acggcgaaag cccggcatct gcatcgacag ggcgcgaatg gcttccgctg ccgtgttcac
     6961 atacaggctg aggcggcggc caaatcgttg taaatccccg tgaaggcaga tgcgtgccag
     7021 tggcggtgac gccagactga atgcgttcgt cgttgccatt tttcggaata cctctcccgt
     7081 ttactcagtt gttcaggcag atggtgaagc agctcaccgt tgccgcagta aatggcggca
     7141 tggttcggta ccgaagcacc aaagcagcac agcagaatat cgcccgcctg tgcagaggac
     7201 aggggcaccc ggtaaaagcc ggtgaccgcc atattgtcca ggtaaaggtt ctgaccgttg
     7261 cgccaccagt catcctcgcg atgaaaatcc ggcatttcag tccccgccag atgataagca
     7321 tcccggaaca gcgtgtaaca gtccgtcacc ccgtgctcaa agcgccgtcc tgtcagatgt
     7381 ggcacacagc ggaatttgtg aatgtcaccc cggcagacca accaccaggg cagtgcgctt
     7441 tttatctgca accgccggtc agcctcgctc agccagggca gcccaccggg atgactgtgg
     7501 accagtgcca caatctcccc ctgcatctct gcccgcagcc agtcttccgg tgcgatacga
     7561 aaatacgcct ccggctctgc ggaaatattc acacaaggga tataccgctc cccctccggc
     7621 gtgcttatca cgaagccgca cgactccgca ggcgcacacc gccgggcatg tgccagaatc
     7681 gctgattcag tctgtgtcat aaaccgggat ttactgcgaa agtttattaa tggaaaggaa
     7741 accgccaaaa ttgccgacat tcctgcgcag ttcacacccg cgcatgcact tgctgcatct
     7801 gtccttacgg atatccgtgg tggggttgtc gaactcatcc gccaccgcag gaccgttata
     7861 cccgcattca tctccccggt aatcccacat acaggtgttc gccagcatga tgcgaccggg
     7921 aaacagcgcc ccgtccgtct cggtcggtgt agccagcaca aacgaggccg tcatggctgt
     7981 cagctgcgac atctgctcca ccacccagcg gtcactcagc tcctgctccg ggtccgcctc
     8041 cggattgccc gcaacgaaat tcaccgcatc cagaaaacgg gcatacaccc ggcggcggac
     8101 caccgtggcc cccaccagac tctgcaggtc ttccgccatc ccggtgacca gaccgaacag
     8161 attggacacc gtcagcgacg gtctggcact gctgccccgg ccgttcatct caaagccgct
     8221 gccgtcaatc gggtatgcct catacttacg cccctgccag gtgaccggct cccctttttc
     8281 attcagctca ttacagaaaa aataccgctc accaccctgt accgtcaggt cgatttccca
     8341 gagtaccacc cgcggtgact gctctgactt aaccgactcg ttcagacttt cttcgcgaat
     8401 atcctgcatc agttcaccac ctgcttaaac tccgcgctga actcaacgcg caacatcccg
     8461 acccgcgcag accacccggc acaggtcacc tttatctgcc ggtatgcata gggtggcttc
     8521 cacaaaaatg ccttccagcc accgtgctct gccaggaacg cttccagatg ccgggcctcc
     8581 tcccgggtca cggaaagcgt caccctgtat gttttcaggt cagcattcag ccctgccgcc
     8641 atacgctgtg agtacccgtc accaaaacgc acttcacgca ccgatggctg cgagttcacc
     8701 tccatatccg gcttcacttt ccagcgaaag gttttcatcc accgctccct gataacatac
     8761 cgccatcacg caactgcagc cggagttcat cctgtgcccc cttgcgggcc atctcataca
     8821 ccgctttcat cagctgcggc cctgcccgcc cgttggggcc gtcgttctga atcaccacgt
     8881 gattgttctg attaaaatta atgccttccg cccgccgcat ctgcgccgga cttccggcac
     8941 cgccgacata accaccttcc gcatagcccc gcatcaggcg gtacagattg ccgacaccaa
     9001 tccggctggt cgcctccttc gtgaagacaa actccccgcg atgaacaatc cccgcaggtt
     9061 catatttacc ccccgtcccc gtaaatcccc cggtcgcgaa atggaagttc gccgccgcag
     9121 cctgaatggc cgtccccgtg gaggcagacg caccaccacc gaaagcaccg ccaatggcgc
     9181 tgccgatact cccgactatc cccaccatcg cctgcttcag aaaaatctct gtcagcatgg
     9241 acagcacaga acgggtgaaa ccacgccagt tctgttcgct gccgatcagc atcgctgcca
     9301 tattctgtgc aataccgtca aaggtctgcg tggccgcgtt tttaacctgc gaaaaactgt
     9361 ccgtcgcact ttccgcccac tcgccccagc cggacttcat cccggccatc cagcttccac
     9421 gaagctgctc ctccgcagac caggtgttct tcagtgcaga tgtggccttc gccagcgcag
     9481 ccggattatc accgtacacc tcacgaaggc gctgctcttc cgactcccgc tgcgcctgac
     9541 ggtcggtgag tccgcgggct tttgcgctga ttgccgcctg cttcgcgctc tgctgctgtt
     9601 caaaccgcgc cgcctgctgt gccagctcat tcagccgttt ctggtgttca atcttgtcgc
     9661 ccagctcagc cagctggcgt ttgtactcca gcgtttcttt ctcatgggcc agcagggatt
     9721 tttcctgctc agataactgc cgtttcgtgg cggcctcttt caggaccaca tactgatttt
     9781 ccgctttcca taaatcacgg cgctgctggc tgattttttc attcgcaccg ctgtgcttct
     9841 ccagcgtcct gagctcagtt tcaagcgcca gcagggctgc atgcgcctgg tcttcctggc
     9901 gctcaccggc agacaccttc acaccggacg gctttttctg cgtcgactca taatcctttt
     9961 ttgccgacgc catcagcgtg ttgtaatccg cctgcaggat tttcccgtct ttcagggcct
    10021 tattcagctc ttcctgccgg gcggtatatt tctccagtgg cgtcagcagg cgctcatacg
    10081 ccttctgcgc ctctccggta tacttcagct gtgacgactc acgctcagcc ctgtcccttg
    10141 ccgccagttc accggctttt tccatatccg actgcagcgt tgccgctgcc agacccagac
    10201 gggcattttc ccggtcatcc catgcgccct gaaggttggc ccggaaagag gaggttttac
    10261 cgcggcgctg gctccgactc tggtaccact gccatttttt atccgcctca tcaaatgcct
    10321 tctgcgcact ggcgagcata tccgctgagg attccggacg accgatatcc agaatggcat
    10381 cccacatcga tttgaatgcc ttccctgttt tatccgccca ggtctccagt gttcccatgt
    10441 tttctttcag gcgacgggtc tgctcatcaa agcctttcgt ggcgatatcg ttcgccgcct
    10501 gcaatgcccc ggcctcgtct ccggaacgct gcagctgtgc aacatacgca atctgctctg
    10561 ccgtcacgtt acggaactgg cgcgccatcg ccatcagtcc cgacgtcggg tcagtggtca
    10621 gcttcccgaa ggcttcagcg actttatcca cctccacacc ggatgcagac gcaaaacgcg
    10681 cgacactctg gttgatggca tcaaactgtt caccaccacg cacaccggca ttcaccaggg
    10741 ctgccagtga ctctctcgcc tggttaaacg tcagccctgc tgcctgcccg gctcttgaga
    10801 gagtcagcat acgatcggca gtcagtccgg actgattacc ggaaagaacc agggttttat
    10861 taaacgctga aagcgtggaa tctccctggt accaggcgta caccagtgca cctgtcgcca
    10921 ccgccagcga ggtgaccccg accatcggca gggtgatcgc atcggcaagc cccctgaaca
    10981 tggggatcat cccgccgaag gagtccttca cctgaccgcc ctgttgcagc aggatcagcc
    11041 agggattctg accaccggca agctgcgtgg cgatatccgt aaactgtgcg ggcagggttc
    11101 gcatggccgc tttatactgc ccgacggaaa tcccggcttt ttgtgcagcc agcgcctggc
    11161 ggctcaggcc ctgttcaaca gcactggcgg tttttctggc gtcggtatcc agacctgaaa
    11221 aatgacgcct tacccggctc atctgctcat cgaaacggac agcatccaga ctcaggtcaa
    11281 taacaagatc accaaccggc tgggacatat ctcacacctc ccggaatccc cgctgaagcc
    11341 atcattaatg cggcatcatc caccatgaca tccgccacat ccgcagacga taaaatatcg
    11401 cgccctccgt ccccaccgaa ccggacgcct ccggcaagtc ctgccgcttt ctgcatcagc
    11461 attttgtcct catccggcct ctccacctgc tcttcctcat gccgggggac aagcagactg
    11521 aaatcagagg gatgcatatc cggatcgcaa aaaaacaggc tgagtacagc gtacgtcagc
    11581 ccggaaaaat gcatatccag ctgggtatcc tgaaaataat gcgtgcggta aaaacggcgc
    11641 cagtcggcat attcggtgga tgtcatcccg gcaagcatgg cgcgccagtc gggtctcccc
    11701 atctcacgcg ccagtctgag ggcaaagttc agctcgccgt cgaagacttt cccgcagaaa
    11761 aatcatcatc agtcagcgtg ttatttttcg ccacttcagt aatatcagta tccggacgaa
    11821 cagcttcgat catcccggac aggcacaaca ccacgtcttc cgcccgggca atggcatcgg
    11881 caggccaggt ggtgagcact tcctgctcta tcttcatcac ggcctcattc attgacggtg
    11941 actgcgtttt ctgtggatgg ttatgccaca gggacatcgc caccagaaac gcgccggttc
    12001 tgacgagatc ttccacgctt acctgcaggt tgccgctgga ttctgcctgt tctgcacgcc
    12061 gtttcaggag ggcaagatgc tcgatacgct gcagcgcaga caattcggaa agcgtgacag
    12121 acacaccgtt atattcaaat tgttctgttt tcagaaacat gtattacctc cgtttaccct
    12181 gcagcgcccg cttcagtaac ggtgacttca gccactgcgg cgaactgacc atttccgctc
    12241 accacaggga tctgcacctt acctgtcgcc acgccgttta ccgtaattgt catatctttc
    12301 acactaatgg tggctttcga cggatcggcg gaaaccgctc tgaacgtctt gtcggttgca
    12361 ctttccggct caaaagaaac cgtcagggtg gttgttttcc cttttgccac cgtaccggat
    12421 gtcggcgtca ccttaatcgc actgaccggc gtaattttgc tgcgttcttc cgctacagaa
    12481 ggtttaccca cgttagtgac tttcaccgtg cgggtgatca cttctttcgc cgtcacggcc
    12541 ttaccgatac tgctgaccca gccacgaaac acatccaccg tgccattcgg aaaacggatt
    12601 ttataggccc ggacatcgcc gctttcaaac cagcctataa gccctttctg gccttcctct
    12661 cccggtttcc aggccagcgt aaaactggta tcacctgcag atttctgccc ctgcccggtc
    12721 gcgctccagt ccgcgtcttc atcatccagg tagttatcat cataggattc agccgtcatc
    12781 tcgcccggcg tcagatcctt caccttagcc agtcgctgcc agtcatcgtc tgacaacggg
    12841 tttgcataag catcagcctt gccgttgtaa acccacagag tggtaccggc accttttacc
    12901 ggctccaggg gatttggtgt tgccatatcg tcctcacatc tcgtatgtaa tggaataagt
    12961 cagatccgca gagctccata acgccatatc gtcatcacga cgatactcat agccctgcgt
    13021 aaccatcgtg gtaatcagtc ctgccagtgc agggatcgca gtcatcgccg gataaatccg
    13081 gctttccatc cactgatcga gctccgaatc aggtacctgt gccggtaaaa acacctcaat
    13141 atgcagtgtg gcccgccagg tatctgcatc cagctcttca ccggtatact ctgcatccgt
    13201 cagataaacc gcgatcgcag gaaaatcctc ttcgtcaaaa acaacggggc gaccatcaaa
    13261 cagcgtcgcc ccgtgttcat gctgctcgag tgcatccagc actgcggcac gaatgtcagt
    13321 gtgtttcatc gttttatcgc aatcctcagt tgttgtttca gcgcgtatgc cagttcttta
    13381 ggcaggcgtt cacgccggat acggtcaaca ttctcatcaa atgcctgttt cagtggggcc
    13441 gccatcggga ttttcaccac ctgaatggga aggcgattac gctttttcct tcccttgtcg
    13501 tcattgccct cctcatatct ggcctgggga agacgttgca taacatgcca gcgcccatta
    13561 tttaatcgct ggataaatgc ccgctgataa cgatgctgac cggctttgag tatgctgttc
    13621 ggacgacgcc ccagcattct gatccccagc ttaatcacag ggagatcacc gcggttaacg
    13681 ataattctgg cattcggatt tctgaccgtc gcccgtttca gtctggaccg ttcctttacc
    13741 agtttccgtc tcaccctggt ttcccgggca acctgtgacg aagactgatt aatcgccgtt
    13801 gtggccacgc ggttaatggt cattgcagaa gcagccggaa tggcgttttt acgaacccgg
    13861 ttcagattgt caatcgcctg atcaagccct tttatcgcca taattcaccc tgcgtttatc
    13921 gtcgccggtt aactgcgggt ggttgcccac ggttgagcca gagataacag ctgcccccgt
    13981 catccggag
//
LOCUS       DAZPLI010000104        13902 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZPLI010000104 DAZPLI010000000
VERSION     DAZPLI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13902
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.105"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            241..1494
                     /gene="kch"
                     /locus_tag="ACHUU3_002925"
     CDS             241..1494
                     /gene="kch"
                     /locus_tag="ACHUU3_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR2082939.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1549..1722)
                     /locus_tag="ACHUU3_002926"
     CDS             complement(1549..1722)
                     /locus_tag="ACHUU3_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR2082940.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1865..3325
                     /gene="cls"
                     /locus_tag="ACHUU3_002927"
     CDS             1865..3325
                     /gene="cls"
                     /locus_tag="ACHUU3_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR2082941.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3360..3689
                     /locus_tag="ACHUU3_002928"
     CDS             3360..3689
                     /locus_tag="ACHUU3_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR2082942.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3742..4746)
                     /gene="oppF"
                     /locus_tag="ACHUU3_002929"
     CDS             complement(3742..4746)
                     /gene="oppF"
                     /locus_tag="ACHUU3_002929"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR2082943.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4743..5756)
                     /gene="oppD"
                     /locus_tag="ACHUU3_002930"
     CDS             complement(4743..5756)
                     /gene="oppD"
                     /locus_tag="ACHUU3_002930"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR2082944.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5768..6676)
                     /gene="oppC"
                     /locus_tag="ACHUU3_002931"
     CDS             complement(5768..6676)
                     /gene="oppC"
                     /locus_tag="ACHUU3_002931"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR2082945.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6691..7611)
                     /gene="oppB"
                     /locus_tag="ACHUU3_002932"
     CDS             complement(6691..7611)
                     /gene="oppB"
                     /locus_tag="ACHUU3_002932"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR2082946.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7697..9328)
                     /gene="oppA"
                     /locus_tag="ACHUU3_002933"
     CDS             complement(7697..9328)
                     /gene="oppA"
                     /locus_tag="ACHUU3_002933"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR2082947.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10066..10713)
                     /gene="ychE"
                     /locus_tag="ACHUU3_002934"
     CDS             complement(10066..10713)
                     /gene="ychE"
                     /locus_tag="ACHUU3_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR2082948.1"
                     /translation="MIQTFLDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11191..13866
                     /gene="adhE"
                     /locus_tag="ACHUU3_002935"
                     /gene_synonym="adhC"
     CDS             11191..13866
                     /gene="adhE"
                     /locus_tag="ACHUU3_002935"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR2082949.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3655 a         3436 c         3232 g         3579 t
ORIGIN      
        1 ttatagccgg tcagtgcatt cactaccaca agcagataat aactttatct gtatttcttt
       61 atatgatgtg agcatattca ggaaaaatga cctttcctga ggtacagttg ctcgctcaaa
      121 tcctttgatt actgcggata aagtcatcta ctattgttta acacattcac gcgctgcatg
      181 tatgttcaga aaatgtgcac gctaagataa ttaaacaaaa ttgtggttac agaggtatca
      241 gtgagtcact gggctacatt caaacaaaca gcaacaaatt tatgggtgac ccttcggcac
      301 gacattctcg cgctggccgt ctttttaaat ggattgctta tttttaaaac aatctatggt
      361 atgtcggtca atttgcttga tattttccat atcaaagcat tttcagagtt ggatctctcc
      421 ttgctggcaa acgccccact atttatgctc ggcgtctttc ttgtcctgaa ctccattggc
      481 ttactgttcc gggcaaagct cgcatgggca atcagtatca ttttgttgtt gatagcgcta
      541 atttacaccc tgcattttta tccctggctg aaatttagta ttggattttg catttttacg
      601 ctggtgtttt tgctgatact gcgcaaagac ttctcccaca gtagcgccgc agccgggaca
      661 atttttgcat ttattagttt cacgacatta ctgttttact caacctacgg tgcgctttat
      721 ttaagcgaag gttttaatcc gcgaatagaa agtttgatga ccgcgttcta tttttcgata
      781 gaaaccatgt caaccgtcgg ctatggcgat attgtccctg tttctgaatc agcacgattg
      841 ttcactattt cggtcattat ttccggcatt accgtttttg ccacatctat gacctcaatt
      901 tttggtccgc ttatccgcgg gggattcaac aaacttgtaa aaggaaacaa tcatacaatg
      961 catcgtaaag atcattttat tgtttgcgga cattcgattc tcgccatcaa tacgatcctg
     1021 caactgaatc aacgcggaca aaacgtaacg gttatcagca acttgcctga agatgatatc
     1081 aagcaacttg agcaacgctt aggcgataac gctgatgtta tccccggtga cagtaatgac
     1141 agttcagtat taaagaaagc gggaatcgat cgttgccggg ccattctggc gctgagtgat
     1201 aacgatgcag ataacgcgtt tgttgtactc tcggcaaaag atatgagcag tgatgtcaaa
     1261 acagttctcg ccgtcagtga tagcaaaaac ctgaataaga ttaagatggt acatccggat
     1321 atcattctct caccgcaact gtttggcagc gaaattctgg cgcgagtttt aaatggtgaa
     1381 gagattaata atgatatgct cgtttcaatg ttgttgaact ccggtcatgg cattttcagc
     1441 gataacgatg aacaagaaac gaaagctgac agtaaagaat cagtgcaaaa atagagttta
     1501 aagacgaaag agtaaactta ttgggcaccg caatggtgcc cagtatattt agatattgta
     1561 gatcgcaaac aataacgagt tacgctgttt gtttagaatg cacttcctga tgctgtggat
     1621 acgcatattt cggcgcgatt gcccctcaag ccaacgcgat ttacgtcggc tagcctgacg
     1681 ctgcatgcgc cagcgcccca cttccgttct actccgcttc atgttaacta ctctatgcaa
     1741 taacagaatg gtcattatac ccccaccccc gacgctgacc agtgcttttc ccgcgttttt
     1801 atttgccaga tgaatccata tgcgtaaact cataacaatg cgctttcaaa aggatttcta
     1861 atctatgaca accgtttata cgttggtgag ttggttggcc attctgggat actggttgct
     1921 cattgcaggc gtaactttac gcattctaat gaaacgacgc gcagttccct ccgcgatggc
     1981 ctggctgttg attatttaca ttctgccgtt agtcggaatt attgcctatc ttgccgttgg
     2041 cgagctccat ttaggcaaac gccgcgctga gcgcgccaga gcgatgtggc cttccaccgc
     2101 aaaatggctt aacgacctta aagcctgtaa gcatatcttc gccgaagaaa atagcagtgt
     2161 cgctgcgcca ttattcaagc tttgcgagcg tcgtcagggg atcgctgggg tcaaagggaa
     2221 tcagctacaa ctgatgaccg agtcagatga tgtgatgcag gcgttaatcc gcgacatcca
     2281 gctcgcgcgc cataatattg agatggtgtt ttatatctgg cagcccggcg gcatggcgga
     2341 ccaggtggct gaatcattaa tggcggctgc acggcgtggt attcattgcc gattgatgct
     2401 cgactccgcc gggagtgtgg cttttttccg cagcccgtgg cccgagctaa tgcgtaatgc
     2461 cggtattgaa gtggtcgaag ccttaaaggt caatctgatg cgtgtgtttt tacgccgtat
     2521 ggacctgcgc caacatcgca agatgatcat gatcgataat tacatcgcgt acaccggcag
     2581 catgaatatg gtcgatcctc gctacttcaa acaagatgcg ggcgtagggc aatggattga
     2641 tctgatggcg cgtatggaag gccccatcgc caccgcgatg gggattattt attcctgcga
     2701 ctgggagatt gaaaccggaa aacgtattct gccgccacca ccagatgtca atattatgcc
     2761 gtttgaacag gccagcggtc acaccattca cacaattgct tctggccccg gctttccgga
     2821 agatctcatt caccaggcat tattgactgc ggcttattcg gcgcgtgaat atttgatcat
     2881 gacaacgccc tactttgtgc caagcgatga tttacttcat gcgatttgca cggcggcgca
     2941 gcgcggggtg gatgtcagta ttatccttcc gcgaaaaaat gactcgatgc tggtcggctg
     3001 ggccagtcgc gcattcttta cggaactgct tgctgctggg gttaaaattt atcagtttga
     3061 aggcgggtta ctgcatacca agagcgtgct ggtcgatggc gaactaagtc tggttggcac
     3121 cgttaacctt gatatgcgta gtctgtggct gaatttcgag attaccctgg caatcgacga
     3181 taaaggtttt ggtgcagacc ttgccgccgt tcaggacgat tatatttcgc gttcacgtct
     3241 gctcgatgcc cgtttatggc taaaacgtcc attatggcaa cgtgtcgccg agcgactgtt
     3301 ttacttcttc agcccgttgc tgtaaaacgt cgccaacaga ggttaaacag gtagtgacta
     3361 tggatatgga tctaaacaat cgcctgactg aagatgaaac gcttgagcag gcctacgata
     3421 tttttctcga actggctgcc gacaatctcg acccagctga tgttctgctg ttcaatcttc
     3481 agtttgaaga gcgcggcggc gcggagttat tcgatccggc ggaagactgg caggaacatg
     3541 ttgatttcga tttgaacccg gacttttttg ccgaagtggt gattggtctg gcggacagtg
     3601 aggatggtga aatcaacgat gttttcgccc gcattctgtt atgccgcgaa aaagatcaca
     3661 aactttgcca tatcatctgg cgggaataaa taatcgcttt cggcgtgaaa aagggctgac
     3721 aactgtcagc ccttattgtg tttaaagcgg atcgactttc aggcaagaaa cggcgtgtct
     3781 gaaactccct tccagcacag gacgtgtttt ggcgcactcc ggaccggcaa tcgggcaaca
     3841 ggtacggaaa acacaaccgg aaggcgggtt gatcggcgac ggtaattccc cttccagtaa
     3901 ctggatggtt ttgttcttct ccagatccgg atcaggtatg gggactgccg acatcaatgc
     3961 cctggtgtaa ggatgtagtg gattgtggta gacctcatca taggtcccca gttctaccgc
     4021 atggccgaga tacatcacca acacacgatc ggaaatgtgt tttaccacgg ccaggtcatg
     4081 agcgataaaa attaatgaca atcccatctc acgttgcagc tgctggagca ggttgaccac
     4141 ctgcgcctga attgacacgt ccagcgccga aatcggctca tcgcagataa tcagcttcgg
     4201 ttcaagaata agagcacgag caatcccgat acgctggcac tgcccaccgg agaactcatg
     4261 cggatagcgg ttaatcaggt taggcaataa cccgactttc agcatcatcg ccttcacgcg
     4321 ctcgcgaact tcctggcgtg acattttcgg atgataagta cgcagtggtt cagcgatgat
     4381 ctcgccgatg gtcatacgcg ggtttagcga tgccaacgga tcctggaaaa tcatctgaat
     4441 atcactgcga acggcacgcc attcatcggg cttcatgccc agcaactctt tacctaacca
     4501 ggcaacatga ccgtcggtcg ctttaaccag gccaatgatg gcgcgagcaa aggtggactt
     4561 accgcatccc gattccccta ccacacctaa tgtttcccct tcatacaggc gaagcgtgac
     4621 accatcgacg gctttgagag ttttcggcgg ttgccagaac cactgtttgc catctttgat
     4681 ttcaaagtgc actttcagat cggcgatttc gaggaggact tttcttcctt cggttacagc
     4741 attcataaca gttcctccac cggtttaaag caagcacgca gacggccagg cgtaaactct
     4801 tccagcggcg gtgcgctact acaaatttcc atcgcatgcg gacaacgtgg ctggaacggg
     4861 caacctttcg gtaatcgcag caggtttggc ggattaccag ggatggtcaa cattgtttca
     4921 ccttccgcat cgagacgcgg caccgcgttg agcaaaccga tagaataagg atgaacgggt
     4981 tgataaaaga catcgcgcgc gttgccatat tccatcgtgc gcccggcgta cattaccagc
     5041 actttgtcgc agatccccgc caccacgcca agatcgtggg taatcataat gatggcggta
     5101 ttaaattccc gcttcagttc atttaatagc gtcatgatct gcgcctgtac ggtgacgtcc
     5161 agcgcagtag tgggttcatc cgcaatcagc agcttaggtc gacatagcaa tgccatcgca
     5221 atcataactc gctgacgcat gccaccagaa aattcgtgcg ggtacatttt catgcgttta
     5281 cgtgcttccg gcatttttac cgcatcgagc atccgcaccg actcttcaaa cgcctcagct
     5341 ttgctcatgt tcttatgcag catcagcact tccatcaact gctcaccgac gcgcatatag
     5401 ggattcaacg aagtcattgg gtcctgaaaa atcattgaga tttgttcagc gcgcagttta
     5461 ttgagttcac gctctggcaa attgaggatt tcacgcccat tgaaggttgc cgatccgcca
     5521 atacgtccgt tggcagccaa caggcccatc aacgcaaatg cagtttgcga tttacccgaa
     5581 ccagactcac ctacaatgcc cagcgtttct ccggcacgta gagaaaaatt caaatcattg
     5641 accgccgtga catcgccgtc cggggtacta aaggtgacac gcaaatcttt cacgttcagc
     5701 agtgcgtcag cctgttgttg tgcgagcggc acagttgcag tttcaattac gctcatggct
     5761 gcactcctta acgatctttc gggtcgagag catcacgcaa gccatcgccg ataaagttga
     5821 aacaaaacag cgtcaccacg aggaatcccg ctgggaacaa caataaccat ggagagactt
     5881 ccatcgagtt cgcgccatca ctcagcaatg caccccagct gcttaacggc tcttgcgtac
     5941 ccaaccccag gaagctaagg aaagattcaa agaggatcat gctgggcacc agaagtgatg
     6001 cgtagaccac caccacaccg agtacattcg gcacaatgtg gcgaataaca atgcccgatg
     6061 tcgatacacc gccaacttgt gccgcctcaa taaactcttt gcgcttcaga ctcagggttt
     6121 gtccacgcac aatacgagcc atatccagcc aggaaaccat gccaatcgcc acgaaaatca
     6181 gcaggatgtt ttgaccgaaa aaggtcacaa gcaaaatgac gaagaacatg aatgggaagg
     6241 agttgaggat ttccagcaga cgcatcatta ccgaatccac tttaccgccc agataaccgg
     6301 aaagcgaacc gtaaagtgtc cccacgacca ctgccaccag tgccgcagca acaccgacca
     6361 tgagtgagat acgcccgcca atcgcaacgc gcacaagcag gtcgcgaccg gatgagtcag
     6421 taccaaagta gtgaccggac tccatatccg gggcgctgga catcatcgcc cagtcagtat
     6481 cgtcataggc aaactgcgaa agcatcggtg ccaggattac aaataacgcg atcagcacca
     6541 gcactatcag actggcaacc gccgcacggt tatgcataaa acgtcgacgt gcgtcctgcc
     6601 acaagctgcg cccttcgacc tccagctttt cactgaaatt ttccagcgtc tcgctgtttt
     6661 tcttacttaa catcatcgcg agctccagta tcagtaacgg attttcgggt cgataaccgc
     6721 atatagcaca tcgacaatgg cattaaacaa aatggttaaa gcaccaacca ggatggtcag
     6781 gcttaacact aaggaatagt cacggttcaa tgcgccatta acgaacagtt gtccaatccc
     6841 cggcaaacca taaatggttt cgataaccat agaaccggta ataatgccga caaatgcggg
     6901 gcccatataa gagagcacgg gtaacagagc aggttttaat gcgtggcgta aaatgatccg
     6961 ccgcataggt aaccctttcg cccgggcagt acgaataaag ttggagtgta atacttcaat
     7021 catagagcct cgggtaatac gcgcaatact ggcgatataa gcgagtgaca acgccaccat
     7081 cggcaatatc atgaatttaa gcgccccacc attccagcca ccgcccggca gccaatgcaa
     7141 aatgatcgca aatatcatga ctaataatgg cgcaaccaca aaactgggga taacaacccc
     7201 ggtcattgcc agccccatca cggtatagtc ccatttggtg ttttgtttta atgcgacaat
     7261 aacgccagca ctaacaccca gtattaccgc aaggaaaaat gcggcggctc ccagtttggc
     7321 agaaacggga aaactggatg caaccaggtc attgaccgaa taatctttat atttaaacga
     7381 tggaccgaaa tcaccgtgcg ccagttgttt caggtagctg aaatactgtg tcatgatcgg
     7441 atcattaaga tgatatttcg cttcgatatt ggccatcact tccggcggta aagtacgttc
     7501 gccggtaaaa gggcttcccg gcgcgaggcg catcataaag aacgaaatag taataagaat
     7561 aaatagcgtc ggaatcgctt ccagacagcg acgtagaata aattttaaca ttgccctacc
     7621 ttctggcctg tgccttctgt aatgcgataa aaatcagaca ccgtggagca ggacactcct
     7681 gccccacgta ttgccattag tgcttcacaa tgtacatatt ccgggtgtag gtattatcca
     7741 gcggatcttt gccggtatag ccaccaaccc acggtttcac cagacgcgca ttcacgtagt
     7801 aataaacagg aacaatggcc gaatccttat ccagctgttg ttctgcttta gtgtacagag
     7861 ctgtgcgctg cgcctcgtca gtcactttca gcgtttccgc cataatgctg tcaaaggccg
     7921 ggctcttata atgcgcggta ttcatcgagc tgttcgacag catggtgttc aggaaggaag
     7981 ttggttcgtt gtagtctgca caccagcctg cacgggccac atcaaaagtg ccctggtgac
     8041 gggtgtcgag gaacgttttc cactcctggt taaccagttt gacgtttaca ccaatgtttt
     8101 tcttccacaa tgaagaggca gcaatcgcca gctttttatg cagatcagag gtgttataca
     8161 gcaggttgat ggtcaacggt ttgtctgcgg tataacccgc ttcagccagc agttttttcg
     8221 cttcttcgtt acgtttttcc tggctccagc caaaccattc tggctgagtc aattttgcgc
     8281 catcagtata cggtggagtg taaccatagg cgggcatgtc gccctgcgct ttcactttat
     8341 taacaatgat atcgcggtcc atacctagtt tcagtgcggt acgcacacgc acatcgttga
     8401 atggcggttt ctggttgtta atttcgtaat agtaagtgca caggtatgga tcaacgtgaa
     8461 cttcgtccgg gatctctttt ttcagcttct ggaacaattc gatcggcatg ttgttatacg
     8521 tcatgtcgat ttcaccacta cggtagcggt tgacatcggt aacttcagaa gcaataggca
     8581 aataggttac ctggttaata acggttttcg cgttgttcca gtaggtcggg ctgcgttcaa
     8641 gaacgattcg ttcgtttacg acccaatctt ttaaggtata ggcaccgttg gtgacgatat
     8701 taccaggctg ggtccatttt tcgccgaatt tctcgatagc ggcttttggc accggtgaag
     8761 ttgatgggtg aacaagtaat ttatagaagt acggaacggg ttcacttaag gtgacttcta
     8821 atgtgtgatc atcaatagct ttcacgccga gatcggtaat cggttttttc ccttcaagaa
     8881 tttcatcaat accggcgata tgcccatatt gcagataact ggcatacgga gaagcagtgt
     8941 tcggatcaac agaacgttgc cagctataca caaagtcttg tgctgtgact ggcgtgctat
     9001 cagaccattt cgcatcttta cgcaaatgga aggtccagac tttcgcgtct ttattatccc
     9061 aggattcagc gacgccaggt gctggatgac cgtcaagatc gctgaccagt aagccttcaa
     9121 acaggtctcg gctgatatta gactccggaa caccttcaat tttgtgcgga tctaatgact
     9181 gaacttctga accattgtta cgtaccagtg tttgtttttc cgccagtgtg acgcctgcgg
     9241 gtacatcagc tgccagcgcg acattccctg ccattagcgc agccagaacg ccagctgcta
     9301 ctaaacttct cttggtgatg ttggtcattg ttttttggac tccctcatta taattactgg
     9361 tattaaccag catgtgtaat cccggattgg gaccctgcac aggctcggag actttctgct
     9421 gtcaggttac tttatcgtta cgtattacta tcaccgactt tatttattct ggtgattcag
     9481 acgatcacct tatgtcgatt cgtactgtgt ctcccctgtc gagacacatt attctttcgc
     9541 gacgctcttt aattgttaat tattctcatt accaataaag aatcgtctgg cggttgcccg
     9601 gaaagtacca aatgcggttc ttactcgcca atacattttg caaaattgtt aagcaattct
     9661 cttttattga ccgtatcaga atctatagct tgcatccatg ccttaacaaa catctttcaa
     9721 ttcaatcaaa tagagattgg ctaagcatgt ctatcttttc tgattacagt tccagtagtg
     9781 aaatgcacaa taatttaaca attgattatt atttagcact ttcctctacc aaaggcagtg
     9841 gaattactaa tatcatttca attattcttc agcaagccca ggattatgat gtggcgaaaa
     9901 taacatgatg gtgaggagaa gcagttaagg aaatccttat aactaactgt aatttgagaa
     9961 gaatattttt acctattcct tttgcttttg ataactgcgc gggaacaaag taaaaataat
    10021 gacgaagtaa acggagcatg gcagctccgt ttcatttgaa aggaattaat taagcaggcc
    10081 ggggaaaatc cccttaatac cagtaacgat aaattcaatc cccaatgcca tcagcaatag
    10141 ccccataata cgcgtaatca cgttgatgcc ggtctggcgt aaaacccgta ccagccacgg
    10201 tgccatgcgg aacaatcccc agcaacataa agcgaacaat gcaatagcca caaagaaacc
    10261 aaacagatag ctaatgctgt gataacgcgt accccagacg atggtagaac tgatcgcccc
    10321 tggccccgcc atcaacggca acgccaatgg caccacacca atgctttcac gtaccgcggt
    10381 ttctgatttt tcttgcttgt tctgtttatc ctcgccaagc ttgccgctga tcatcgacat
    10441 cgctatcgtc accaccagga tgccccctgc gatacggaac gaatcaattg atataccaaa
    10501 aagttgcaga atcgtgtcgc cgagaaaaag cgagatccac aagataatgg ccacagacag
    10561 gttggctgta aggttagttt tgtttcgcgc tgccgctgtc tgataactgg tcatgctgat
    10621 aaagacggga ataatcccta ccgggttgac cagcgcaaat aacccgatga aaaatttgaa
    10681 gtaaacggga aaatccaaaa aggtctgaat cacggttagc tccgaagcaa aagccggata
    10741 atgttagcca taaattaagg ttgaaaagac gcgctgacaa tacgcctttt gacagcattt
    10801 ttcacctcct aactacttaa aattgctatc attcgttatt gttatctagt tgtgcaaaac
    10861 atgctaatgt agccaccaaa tcatactaca atttattaac tgttagctat aatggcgaaa
    10921 agcgatgctg aaaggtgtca gcttagcaaa aatttgattt ggatcacgta atcagtaccc
    10981 agaagtgagt aatcttgctt acgccaccta gaagtgacgc attagagata ataactctaa
    11041 tgtttaaact cttttagtaa atcacagtga gtgtgagcgc gagtaagctt ttgattttca
    11101 taggttaagc aaatcatcac cgcactgact atactctcgt attcgagcag atgatttact
    11161 aaaaaagttt aacattatca ggagagcatt atggctgtta ctaatgtcgc tgaacttaac
    11221 gcactcgtag agcgtgtaaa aaaagcccag cgtgaatatg ccagtttcac tcaagagcaa
    11281 gtagacaaaa tcttccgcgc cgccgctctg gctgctgcag atgctcgaat cccactcgcg
    11341 aaaatggccg ttgccgaatc cggcatgggt atcgtcgaag ataaagtgat caaaaaccac
    11401 tttgcttctg aatatatcta caacgcctat aaagatgaaa aaacctgtgg tgttctgtct
    11461 gaagacgaca cttttggtac catcactatc gctgaaccaa tcggtattat ttgcggtatc
    11521 gttccgacca ctaacccgac ttcaactgct atcttcaaat cgctgatcag tctgaagacc
    11581 cgtaacgcca ttatcttctc cccgcacccg cgtgcaaaag atgccaccaa caaagcggct
    11641 gatatcgttc tgcaggctgc tatcgctgcc ggtgctccga aagatctgat cggctggatc
    11701 gatcaacctt ctgttgaact gtctaacgca ctgatgcacc acccagacat caacctgatc
    11761 ctcgcgactg gtggtccggg catggttaaa gccgcataca gctccggtaa accagctatc
    11821 ggtgtaggcg cgggcaacac tccagttgtt atcgatgaaa ctgctgatat caaacgtgca
    11881 gttgcatctg tactgatgtc caaaaccttc gacaacggcg taatctgtgc ttctgaacag
    11941 tctgttgttg ttgttgactc tgtttatgac gctgtacgtg aacgttttgc aacccacggc
    12001 ggctatctgt tgcagggtaa agagctgaaa gctgttcagg atgttatcct gaaaaacggt
    12061 gcgctgaacg cggctatcgt tggtcagcca gcctataaaa ttgctgaact ggcaggcttc
    12121 tctgtaccag aaaacaccaa gattctgatc ggtgaagtga ccgttgttga tgaaagcgaa
    12181 ccgttcgcac atgaaaaact gtccccgact ctggcaatgt accgcgctaa agatttcgaa
    12241 gacgcggtag aaaaagcaga gaaactggtt gctatgggcg gtatcggtca tacctcttgc
    12301 ctgtacactg accaggataa ccaaccggct cgcgtttctt acttcggtca gaaaatgaaa
    12361 acggctcgta tcctgattaa caccccagcg tctcagggtg gtatcggtga cctgtataac
    12421 ttcaaactcg caccttccct gactctgggt tgtggttcct gggggggtaa ctccatctct
    12481 gaaaacgttg gtccgaaaca cctgatcaac aagaaaaccg ttgctaagcg agctgaaaac
    12541 atgttgtggc acaaacttcc gaaatctatc tacttccgcc gtggctccct gccaatcgcg
    12601 ctggatgaag tgattactga tggccacaaa cgtgcgctca tcgtgactga ccgcttcctg
    12661 ttcaacaatg gttatgctga tcagatcact tccgtactga aagcagcagg cgttgaaact
    12721 gaagtcttct tcgaagtaga agcggacccg accctgagca tcgttcgtaa aggtgcagaa
    12781 ctggcaaact ccttcaaacc agacgtgatt atcgcgctgg gtggtggttc cccgatggac
    12841 gccgcgaaga ttatgtgggt tatgtacgaa catccggaaa ctcacttcga agagctggcg
    12901 ctgcgcttta tggatatccg taaacgtatc tacaagttcc cgaaaatggg cgtgaaagcg
    12961 aaaatgatcg ctgtcaccac cacttctggt acaggttctg aagtcactcc gtttgcggtt
    13021 gtaactgacg acgctactgg tcagaaatat ccgctggcag actatgcgct gactccggat
    13081 atggcgattg tcgacgccaa cctggttatg gacatgccga agtccctgtg tgctttcggt
    13141 ggtctggacg cagtaactca cgccatggaa gcttatgttt ctgtactggc atctgagttc
    13201 tctgatggtc aggctctgca ggcactgaaa ctgctgaaag aatatctgcc agcgtcctac
    13261 cacgaagggt ctaaaaatcc ggtagcgcgt gaacgtgttc acagtgcagc aactatcgcg
    13321 ggtatcgcat ttgcgaacgc cttcctgggt gtatgtcact caatggcgca caaactgggt
    13381 tcccagttcc atattccgca cggtctggca aacgccctgc tgatttgtaa cgttattcgc
    13441 tacaatgcga acgacaaccc gaccaagcag actgcattca gccagtatga ccgtccgcag
    13501 gctcgccgtc gttatgctga aattgccgac cacttgggtc tgagcgcacc gggcgaccgt
    13561 actgctgcta agatcgagaa actgctggca tggctggaaa cgctgaaagc tgaactgggt
    13621 attccgaaat ctatccgtga agctggcgtt caggaagcag acttcctggc gaacgtggat
    13681 aaactgtctg aagatgcatt cgatgaccag tgcaccggcg ctaacccgcg ttacccgctg
    13741 atctccgagc tgaaacagat cctgctggat acctactacg gtcgtgatta tgtagaaggt
    13801 gaaactgcag cgaaaaaaga agccgctccg gctaaagctg agaaaaaagc gaaaaaatcc
    13861 gcttaatcag tagcgctgtc tggcaatata aacggcccct tc
//
LOCUS       DAZPLI010000105        13886 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZPLI010000105 DAZPLI010000000
VERSION     DAZPLI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.106"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="ACHUU3_002936"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="ACHUU3_002936"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR2082950.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="ACHUU3_002937"
     CDS             2273..2662
                     /locus_tag="ACHUU3_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR2082951.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="ACHUU3_002938"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="ACHUU3_002938"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR2082952.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHUU3_002939"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHUU3_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR2082953.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="ACHUU3_002940"
     CDS             complement(4321..4521)
                     /locus_tag="ACHUU3_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR2082954.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="ACHUU3_002941"
     CDS             4687..5232
                     /locus_tag="ACHUU3_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR2082955.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="ACHUU3_002942"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="ACHUU3_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHUU3_002943"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHUU3_002943"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR2082956.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="ACHUU3_002944"
     CDS             complement(6529..7353)
                     /locus_tag="ACHUU3_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR2082957.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQILSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHUU3_002945"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHUU3_002945"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR2082958.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHUU3_002946"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHUU3_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR2082959.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHUU3_002947"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHUU3_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR2082960.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHUU3_002948"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHUU3_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR2082961.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /locus_tag="ACHUU3_002949"
     CDS             complement(11316..11969)
                     /locus_tag="ACHUU3_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR2082962.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHUU3_002950"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHUU3_002950"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR2082963.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHUU3_002951"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHUU3_002951"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR2082964.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3361 a         3519 c         3694 g         3312 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagacggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaaggatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagag
//
LOCUS       DAZPLI010000106        13855 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZPLI010000106 DAZPLI010000000
VERSION     DAZPLI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13855
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.107"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(1..738)
                     /gene="dsbG"
                     /locus_tag="ACHUU3_002952"
                     /pseudo
     CDS             complement(1..738)
                     /gene="dsbG"
                     /locus_tag="ACHUU3_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
     gene            1110..1673
                     /gene="ahpC"
                     /locus_tag="ACHUU3_002953"
     CDS             1110..1673
                     /gene="ahpC"
                     /locus_tag="ACHUU3_002953"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR2082965.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1844..3409
                     /gene="ahpF"
                     /locus_tag="ACHUU3_002954"
     CDS             1844..3409
                     /gene="ahpF"
                     /locus_tag="ACHUU3_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR2082966.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3530..3958)
                     /gene="uspG"
                     /locus_tag="ACHUU3_002955"
     CDS             complement(3530..3958)
                     /gene="uspG"
                     /locus_tag="ACHUU3_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR2082967.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4179..5417
                     /locus_tag="ACHUU3_002956"
     CDS             4179..5417
                     /locus_tag="ACHUU3_002956"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFR2082968.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5421..5510)
                     /gene="yldA"
                     /locus_tag="ACHUU3_002957"
     CDS             complement(5421..5510)
                     /gene="yldA"
                     /locus_tag="ACHUU3_002957"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR2082969.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(5648..6058)
                     /gene="rnk"
                     /locus_tag="ACHUU3_002958"
     CDS             complement(5648..6058)
                     /gene="rnk"
                     /locus_tag="ACHUU3_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR2082970.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6288..7094)
                     /gene="rna"
                     /locus_tag="ACHUU3_002959"
     CDS             complement(6288..7094)
                     /gene="rna"
                     /locus_tag="ACHUU3_002959"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR2082971.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            complement(7208..8671)
                     /gene="citT"
                     /locus_tag="ACHUU3_002960"
     CDS             complement(7208..8671)
                     /gene="citT"
                     /locus_tag="ACHUU3_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFR2082972.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8722..9600)
                     /gene="citG"
                     /locus_tag="ACHUU3_002961"
     CDS             complement(8722..9600)
                     /gene="citG"
                     /locus_tag="ACHUU3_002961"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR2082973.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9575..10126)
                     /gene="citX"
                     /locus_tag="ACHUU3_002962"
     CDS             complement(9575..10126)
                     /gene="citX"
                     /locus_tag="ACHUU3_002962"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFR2082974.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10130..11662)
                     /gene="citF"
                     /locus_tag="ACHUU3_002963"
     CDS             complement(10130..11662)
                     /gene="citF"
                     /locus_tag="ACHUU3_002963"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFR2082975.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANQEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            complement(11673..12581)
                     /gene="citE"
                     /locus_tag="ACHUU3_002964"
     CDS             complement(11673..12581)
                     /gene="citE"
                     /locus_tag="ACHUU3_002964"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR2082976.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12578..12874)
                     /gene="citD"
                     /locus_tag="ACHUU3_002965"
     CDS             complement(12578..12874)
                     /gene="citD"
                     /locus_tag="ACHUU3_002965"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR2082977.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12889..>13855)
                     /gene="citC"
                     /locus_tag="ACHUU3_002966"
     CDS             complement(12889..>13855)
                     /gene="citC"
                     /locus_tag="ACHUU3_002966"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR2082978.1"
                     /translation="VDTTVEVFITVTRDEKLITCGGIAGNIIKCVAISESVRGEGLAL
                     TLATELINLAYERHSTHLFIYTKTEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRY
                     AESLKKFRHPGNKIGCIVMNANPFTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYED
                     RLDLVLKGTADIPRLTVHRGSEYIISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLA
                     PALGVTHRFVGTEPFCRVTAQYNQDMRYWLETPTISAPPIELVEIERLRYQEMPISAS
                     RVRQLLAKNDLTAIAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3401 a         3507 c         3688 g         3259 t
ORIGIN      
        1 ataatgatat taagcgtttt ctgatcgggc aaccccacgg cctgttgcag cgtattttcc
       61 ttactcatgt aatagatagc cggcgtgaca tttgccccca gatcgtccat cagtttctca
      121 ttgtcactta acactttcat ttgctctgta cttacgtttg caggcacgtt tagcttaagc
      181 ttgccaccag aggcttcata ttgttgccag gtttttgcgg gatctttgga ggcaagaatt
      241 gccgctgctg tcgccgggct ttctggcttg ataaccccaa ccaacaatgt tcttaattgc
      301 actttgccag aatctaccca cgggcgcgcc tgctgccaga actgtttaca atatgggcag
      361 aacggatcgg cgaagacgta gacaatgacc ggcgcatctt ttttaccgtc gaggatccag
      421 tgggattgtt ccatccgttg ccacatttcg cgtccggctg gtgcgtaaat ttctttttcg
      481 ataagtgtgt tactcaggtt ttcacctttc tcgttgtaca tgtaaccaga gatagcgtgc
      541 ttaccatcgg gagtcaggta ggtagatggt gacgcccata tcctgatact ttccgagata
      601 acctttcatt cctccggggg catcgaacgt tttgatgatt gtaatgccct gtttttcaat
      661 cgcttttact ggggaaggaa gttcctctgc gaaggcgatt gcaggaagca gagccagtaa
      721 aagtatcttt tttaacatta atttgtcctt ttcagtcagt gcaaaagtcg agtaaaaggc
      781 ataacctatc actgtcatag gtaagagctt agatcaggtg attgcccttt gtttatgagg
      841 gtgttgtaat ccatgtcgtt gttgcatttg taagggcaac acctcagcct gcaggcaggc
      901 gctgaagata ccaaagggta gttcagatta cacggtcacc tggaaagggg gccattttac
      961 tttttatcgc cgctggcggt gcaaagttca caaagttgtc ttacgaaggt tgtaaggtaa
     1021 aacttatcga tttgataatg gaaacgcatt agccgaatcg gcaaaaattg gttaccttac
     1081 atctcatcga aaacacggag gaagtataga tgtccttgat taacaccaaa attaaacctt
     1141 ttaaaaacca ggcattcaaa aacggcgaat tcatcgaaat caccgaaaaa gataccgaag
     1201 gccgctggag cgtcttcttc ttctacccgg ctgactttac tttcgtatgc ccgaccgaac
     1261 tgggtgacgt tgctgaccac tacgaagaac tgcagaaact gggcgtagac gtatacgcag
     1321 tatctaccga tactcacttc acccacaaag catggcacag cagctctgaa accatcgcta
     1381 aaatcaaata tgcgatgatc ggcgacccga ctggcgccct gacccgtaac ttcgacaaca
     1441 tgcgtgaaga tgaaggtctg gctgaccgtg cgaccttcgt tgttgacccg cagggtatca
     1501 tccaggcaat cgaagttacc gctgaaggca ttggccgtga cgcgtctgat ctgctgcgta
     1561 aaatcaaagc agctcagtac gtagcttctc acccaggtga agtttgcccg gctaaatgga
     1621 aagaaggtga agcaactctg gctccgtctc tggacctggt tggtaaaatc taaatttcct
     1681 tcgtctttcg cgctaccaac tccggcgcga aatacttcgg aaattcacct aattcttcgg
     1741 gtgctgcggc acccgatttt cttccccgca ccatgatgca agctgcatcc aggtagccgt
     1801 agaggccgct tgcatgatga tgtttaaagc ccaggagata aacatgctcg acacaaatat
     1861 gaaaactcaa ctcaaggctt accttgagaa attgaccaag cctgttgagt taattgccac
     1921 gctggatgac agcgctaaat cggcagaaat caaggaactg ttggctgaaa tcgcagaact
     1981 gtcagacaaa gtcaccttta aagaagataa cagcttgccg gtgcgtaagc cgtctttcct
     2041 gatcaccaac ccaggttcca accaggggcc acgttttgca ggctccccgc tgggccacga
     2101 gttcacctcg ctggtactgg cgttgctgtg gaccggtggt catccgtcga aagaagcgca
     2161 gtctctgctg gagcagattc gccatattga cggtgatttt gaattcgaaa cctattactc
     2221 gctctcttgc cataactgcc cggacgtggt gcaggcgctg aacctgatga gcgtactgaa
     2281 cccgcgcatc aagcacactg caattgacgg cggcaccttc cagaacgaaa tcaccgatcg
     2341 caacgtgatg ggcgttccgg cagtgttcgt aaacgggaaa gagtttggtc agggccgcat
     2401 gacgttgact gaaatcgttg ccaaaattga tacgggcgcg gaaaaacgtg cggcagaaga
     2461 gctgaacaag cgtgatgctt atgacgtatt aatcgtcggt tccggcccgg cgggtgcagc
     2521 ggcagcaatt tactccgcac gtaaaggcat ccgtaccggt ctgatgggcg aacgttttgg
     2581 tggtcagatc ctcgataccg ttgatatcga aaactacatt tctgtaccga agactgaagg
     2641 gcagaagctg gcaggcgcac tgaaagttca cgttgatgaa tacgacgttg atgtgatcga
     2701 cagccagagc gccagcaaac tgatcccagc agcagttgaa ggtggtctgc atcagattga
     2761 aacagcttct ggcgcggtac tgaaagcacg cagcattatc gtggcgaccg gtgcaaaatg
     2821 gcgcaacatg aacgttccgg gcgaagatca gtatcgcacc aaaggcgtga cctactgccc
     2881 gcactgcgac ggcccgctgt ttaaaggtaa acgcgtagcg gttatcggcg gcggtaactc
     2941 cggcgtggaa gcggcaattg acctggcggg tatcgttgag cacgtaacgc tgctggaatt
     3001 tgcgccagaa atgaaagccg accaggttct gcaggacaaa ctgcgcagcc tgaaaaacgt
     3061 cgacattatt ctgaatgcgc aaaccacgga agtgaaaggc gacggcagca aagtcgttgg
     3121 tctggaatat cgagatcgtg tcagcggcga tattcacaac atcgaactgg ccggtatttt
     3181 cgtgcagatt ggtctgctgc cgaacaccaa ctggctcgaa ggcgcagtcg aacgtaaccg
     3241 catgggtgag attatcattg atgcgaaatg cgaaaccaac gtcaaaggcg tattcgcagc
     3301 gggtgactgt acgacggttc cgtacaagca gatcatcatc gccactggcg aaggtgccaa
     3361 agcctctttg agtgcttttg actacctgat tcgcaccaaa actgcataag aagaagtaag
     3421 attcacctgc aattgcttag ccgccggggt caaacctggc ggctttttta tggcattaaa
     3481 aagccctgcc gggatgttcc aggcagggcg gcataaatac ttttatctgt tagcgaatga
     3541 ccagtacagg cagattggcg tggcggatca cgctcgaggc gttagaacct aacagatggg
     3601 tcgaaatcga tgggttgcga gaaccgataa ctacaacatc agcccccagt tcttctgcca
     3661 actcattgac ttcatcccgc acgctaccaa aacggacatg ttgtttaatg cgggaaggat
     3721 cgatggtgaa gtggctgacc atcgtttgca gacgttcttc tgcttcatgt tgcagatgct
     3781 cttcaaaacg acgcacatca gcggcaaaac ggtgcaggct caggctggct gacccgggta
     3841 gtacgtgaag tagatgaata actccgtcat cctgggcgag gaattcagcg tggcgaacag
     3901 ctttgtcgct caattccatt tcaaatacat caactggcat aatgattgtc ttatacataa
     3961 ccctttctcc ctgttaatca tgaacaaatc attcgccatg attataatat ttatccctga
     4021 tatttgtctg gttctttttc cttacgaacg gtttctgtga ttaatatatt ctcactgaac
     4081 accaggaatt ctcccaaaac ctgcggtacc gcccgttttc ccgctgtgat agctaccctt
     4141 aaagactgac tcttttttga actgtctctg gaggttgcat gaaagcattg acttatcacg
     4201 gcccacatca cgttcaggta gaaaatgttc ccgatccggg cattgaacag gctgatgata
     4261 ttattctgcg tattacggca acggcgatct gtggctctga cctccatctt tatcgaggca
     4321 aaatacccca ggttaaacat ggcgatattt tgggtcatga atttatgggg gaagtcgttg
     4381 aaaccggaaa ggacgtaaaa aatttgcaaa aaggcgaccg ggtggtaatt ccgtttgtca
     4441 ttgcttgtgg cgactgtttt ttctgtcgaa tgcagcaata tgccgcctgc gaaaatacca
     4501 atgcgggtaa aggcgctgcg ctcaataaaa aacagatacc agctcccgcg gcattgtttg
     4561 gttatagtca cctgtatggt ggcgttcctg gtggacaggc ggaatatgtc cgcgtcccta
     4621 aagggaatgt ggggccgttt aaagtaccgc ctttgctttc agatgataaa gcccttttcc
     4681 tttctgatat tctgccaacg gcatggcagg cagcaaaaaa tgcgcagatc caacaaggtt
     4741 caagcgttgc agtctatggt gccggtccag tgggattgtt gacaatcgcc tgtgcacggt
     4801 tgctcggtgc ggaacagatt tttgttgttg atcatcatcc ctaccgcttg cgtttcgccg
     4861 ctgaccgcta cggcgcgatc ccgattaatt tcgatgaaga tagcgatccg gcacagtcaa
     4921 ttattgaaca aacggcaggt caccggggcg tggatgcagt aatagacgcc gtcggttttg
     4981 aagcgaaagg cagcaccacg gaaacggtgc tgactaacct gaaactggag ggcagcagcg
     5041 gtaaagcgtt gcgccagtgt attgcggcgg tcaggcgtgg cggcattgtt agcgtaccgg
     5101 gcgtctacgc tggatttatt cacggtttcc tgtttggcga cgcctttgat aaagggttga
     5161 cgtttaaaat gggacagacc cacgttcacg catggctggg tgaattatta ccgttaattg
     5221 agaaaggatt actgaaacca gaagaaattg ttacccacta tatgccgttt gaagaggccg
     5281 cccggggata tgagattttc gaaaaacgtg aagaggagtg ccgtaaggtg attctggtac
     5341 ccggtgcaca aagcgcagag gcggcgcaga aggcggtttc aggtctggtg aatgcgatgc
     5401 cggggggaac aatatgatcg tcaggagtgg ttttcgaggt aaaggacagc catgacgata
     5461 atcgccgcca taatcagaaa tcctatcagg atgtaaaatg cttctgccat ggttattccc
     5521 acaaacgaaa cggaataatt ttgcagcaaa agtgaacagt gagaaccagg aaaaattgct
     5581 gattttgcgt aaagaggatg cgagtgcatc ctctgggcaa agcgagttat cgcttgtgca
     5641 gatgggatta aagcaggtag tcgccagcag cttctggctg gtattcgagt tccagcactt
     5701 caaggtgggt tgcaacgccg cccggaagtt cccagtgaat ggaatcgcca acgcgcagcc
     5761 ccagcagtgc ggcacctacc ggagccataa cggaaagctg agtattgcta tcggtcattt
     5821 ttgccggata caccagcgtg cgcacacgca cttcgccatc gctaagattg cggaatttaa
     5881 cccggctgtt cattgtcacc acgtcgtgtg gcatctcttc tggcgaacac atttgggcgc
     5941 gatccaactc tgcgtttaac gcgtcggcga ttggcaaacc agcataggcg ggttgctcca
     6001 gcagaatatc gatgcgttcg gcatccaggt cgttaatgat gatagttggt ctggacattt
     6061 ttactccatg tcgtcggtac tgcgagtgtc gcagataaac atacccaaaa gaaaaccctc
     6121 accgtcaggc ggcgagggtt taactcacat gatgatactg actgttgctc actctttgaa
     6181 gtgatttgcg tcacattcag ggaattcctc aatgcagcgc attatgtata attcttaatc
     6241 gtcacggttt atggaagacg aattagcgtg ttttgtaaat cagatgatta ataacccact
     6301 ttatcaatca caaaggtttt gccacagttg cctgggtgtg gttgtggcaa gaatgagttt
     6361 gcagaaagcg gagcgttgat ggcgtcagct ttgatcgaga tctgaatttc agtcaaatac
     6421 gcagggttac cctggcacgt tagcttaact gctttcacgt tctcttttcc ccagcttttg
     6481 gcaaaggcgg cgtcaaagtc acggcggctc actgttttac cgtaattatc cgcaaggaat
     6541 tttccggctt cgctctcttt aatttcttga ttcaggcgta ccatcgtacc gaagtatgcg
     6601 tccggatcaa aaccaaagca ggcaccgtgt ttggcatatt cgtagcgttc caggcaggaa
     6661 cgtccgccag ctcctggcat gacttcactt agtttagcgg ccgtttccag tgataacccg
     6721 gtttccggcg atgaacacat ccggctggcg tgcgcttctg gtagattcgg gattgggcga
     6781 gtagcgcaac cgaagcgcat ccagcggcgt tcatcaacac cacgggcagc aaccgattta
     6841 ggcaatcctg gccacagacc atgtacggtc agaaaatcag ctttgttggt cgtttcggtt
     6901 tgtaggcgac attcatctcg ttcgttacga tttcgatcgt gttgactctg gcaaaatccg
     6961 gtttgccagg agagggccag gacatagcga tcaaaatcgc catactgttt tgcctgcaac
     7021 gctacggcgt tggcagaaga gaagggaagc agagaaaccg cgagcaacgc ggcgttacgc
     7081 cagaatgctt tcataatggg tgtggaactc atacatacac tgaatactat ctattaaatc
     7141 ataaaaaagc ccgccaggtg ggcttacctg gcgggcgtga tgatttattc agcgtttggc
     7201 gaacgtatta gttccacatg gcgagaatcg gccagccaac caacagcaac atagagatgt
     7261 aaatcacccc gaagattgcg ccaagacgcc agtaatcttt tgatttcaca tagccacagc
     7321 cgtaaataat caccccagga ccggttgcat acggcgtcag acagcccatg ataccgatag
     7381 acagcaccag caggatacac agttgttcca ttggtacgcc cggaatacct ttaccgacgg
     7441 ccagaataac cgacagcatg gttgcggtgt gcgcagacag gctggcaaac aggtagtgtg
     7501 caaagtagaa caccagaacc agtacaatca ccgttgcgtt tggtgagaat ccttccaggt
     7561 gcgtactcat ggtaccggcg aaccagtcaa taaagccaga acgagtcagg ccgttagcca
     7621 tcacaaccag agttgccagg ttgaccagcg tgttccatgc gctgttatag cgggtaatat
     7681 ctttccaagg cacaacgtgc agggccagca ttagcgaaac tgccagcaga ccaactgcag
     7741 tagcattaat gacttcactg ccaaaaaccc acaaacctaa gctgagcaat acaaggccaa
     7801 tcagtgtcca ctctctgcgt gtcagcgcac ccatggtttt cagttcatca cccgcccagg
     7861 ttgccacttc ttcactgtgt gtgatttccg gtttgtacag cacgtaggaa agccacggcg
     7921 caatgataag caagataacc ccaaccggca ggaagcagag gaaccactgc aaccagctaa
     7981 tctggatacc ggcaattttg ctgacgaact ccagacccag cacgtttggt gccgcaccgg
     8041 tgacaaacat ggacgaactc agactggtac taatgaccat catccacatc aaatagccgc
     8101 caatacgacg cgcggacgga tcgttgggga atgatttaaa cagcggcggc aggtttttaa
     8161 tgaccggaaa aaccgtaccc ccggtacgcg cggtgttgga cggtgtaaac ggtgccagca
     8221 gaatgtcgat aatgacaatc gcataaccca acgtcagcgt gcgtttgccc atgaatttca
     8281 ccaggaaaag ggcaatgcga cgacctaacc cggaaacttc ataccctaat gcaaaaataa
     8341 atgcgccaaa taccagccat accgtggtgc tggaaaaacc agccagaccc catttcagcg
     8401 cctgtttttg cgcattaaac gctgggtcag ctaattcttt ggcatcaaag agcaggtaat
     8461 tactgccaat aacgcaaata gtaaccgcaa taaaactgat cgctgttgcc ggaattggct
     8521 cgaggatcat gccgacaatc attgccacaa acacagcgaa gtaatgccat gcctgtggtg
     8581 gcataccgtc ggggacaggg ataagaaaca tgacacccat caccaccagt ggggccaata
     8641 gtttccatat attatctttt gctaaagaca tacgggttct ccgaaaatta atatttccaa
     8701 atttatcaag tgcttaaata attaaatctg tgctaaaaac caggtaagga tcagcaggtc
     8761 ggcactgccg cctggactga gatttcgttc gatacactcc ctgtcgaact gccggagata
     8821 atcgagatcg gcgggggttc gaatgccccc tttttgcaat aatgtttgcg cctcgcgctg
     8881 tagccagcgc aggcccccct cgccaccgcg cgatgcaacg ttggtatcgc cgttgatcgc
     8941 catcagtagg agcaaggtat cgagcaatgc cagttcagga tctaacccct gatccagcag
     9001 agtgaggtaa tgcggcaagg cgtgattgat caccagtgga taacccgctt cggcttcacc
     9061 gcgtgcgccg gtaaggccaa gctgctggta caaccgttga cctgccgtca gttgtgaatt
     9121 attggtacgc agttctcgat cggtcaggcc acggcagaaa cttgccgccg tagaacaaac
     9181 ggttgttggc gttaccggtt ggttgagttg aagcaaacgg ccaattgccg cacatagtag
     9241 ccctaaagaa aaaatgctgc ctttatgcgt gtttacgccc gcagtggcgc ggaacatatc
     9301 accttcgcaa gccataccaa ttgggcgtaa tccgtggagt accgcttctg gtgctatttc
     9361 cgcactacag gcaccaaatt caatgaaacg gggtagccag ccctgaatcg ccagcgcgct
     9421 gcggtggaaa tcttccagcg ccatatcttt atgtgcaccg cagttaatcc gatccacgag
     9481 gcctggtttc ggtgacagat tgacttcagt cagcatggcg cgccagccca gcagggcgta
     9541 ctcatcgatc aatgacgtcg caagctttgt ggttttagtt gacgttgcag gcatcgacat
     9601 cgttcagcag tgcctccatg cggttgagta aatcggtcag ttgatgggtt tttccacgcg
     9661 cgcagacggc tgcgctttgc tcgcacaaca ggcagcggcg aggcggcagt gaatagtcgc
     9721 ggcgggagag aatttcgcct tcgggcgtca ggacatcgat atcccataac cgcccgagag
     9781 gatgactatg ttcaagctca atggtggcga gcttgaggtc gcgagccggg gcggcaatgc
     9841 tcaacatgcc ctccggcccg ctggcggaaa ccagtgcagc ctgctcctga atttgccagc
     9901 cctgttttgc ggctaaggca cgcaaggctg tcacgccatg attaaaaatt cggcgtgtga
     9961 cctcgctgtc tttaatcggc ccaggcgcaa ccacggtaaa ggagaccagt ggaacaggat
    10021 ggcgcttgag ccagacgtgt tgccgtgctt gcctttcatc ccggctgacg agcagctcgg
    10081 gaattgacac cgcatggtgg ctggcgagtt caggaagcag gtgcatggct tattccttca
    10141 cctgatgcac aacatcgatc accgagccat cgcggtaacg cacaacggca acgacgcggt
    10201 cggtgaattc aatcggctgt ggttcaccgg tcagcagacg cgcgcgttcg cgcagccact
    10261 caatggaaac cactttaatg cccgcttcct gcagacgttc tgccagttcc ggacgtgccg
    10321 ggttaactgc gataccgtgg tctgtgacca gaatatcgac actggagcct ggggtgatgc
    10381 aggtcagcac gttatccacc agagtcggaa tacgaccgcg taccagcggc gcgacgatga
    10441 tggaaagcgc agaggcaatc gcggtatcgc agtgaccacc ggaagcacca cgcagtacgc
    10501 cgtcagagcc ggtcagcacg ttaacgttga actgggtgtc aatttccagc gcgctcagta
    10561 ccaccacgtc gagacgatca accgatgcgc ctttcgaacc ccagttagcg tactggttgg
    10621 cgctgatttc gatgtgattg gggttacggg ccagcgattg cgcagcatgg ctgtcaaagc
    10681 tctgcacatc cagcagtttg cggatcagac ctttttcgtg cagatcaacc atcgtcgcgg
    10741 taataccgcc gagggcgaag tcggcgcgaa tatcgcggct acgcattttg tcttccagga
    10801 aacgggttac cgccagcgat gcgccgccgg tgccggtttg catggagaaa ccttctttga
    10861 agtagccaga gttgacaatc acatccgcag cgctacgggc aataagcagt tcgcgcgggt
    10921 tagtggtcat acgggtcgcg ccagcgccga tttttgcagc atcgccaacg cggtcaactt
    10981 tgacgatcaa atcaacctga tcttgctcaa tgcttgccgg attatgcgga taaggcagca
    11041 gttcttcggt aagcatcaca acctgttttg cgttgtcggc atcaaccatt gcatagccga
    11101 gggagccgca gcaggcttta ccggtgtaac cgttggcatt accgaattca tcacaggacg
    11161 ggacgccgag gaaagccacg tcgatattca gttcgccgct ctgtaccaga tgcacacgtc
    11221 cgccgtgaga gtggatttgc accggttccg caagcagacc acgggaaatt tcttctgcca
    11281 gtggaccgcg caggccggag gtataaatgc gggtaaccac gccctggcga atgtgttcta
    11341 ccagtggcgc atggcaatca ctcagggagc tggacgccag ggtcaggttt ttaaagccca
    11401 tcttagcgat gacgtccatc accatattga cggtcaggtc accgccacgg aaagcgtgat
    11461 ggaaggaaac cgtcatgccg tcctgtaaac cagagcgacg aatcgcttct tcctggttgg
    11521 cgcacagttt gcgatcgcgc gctttttcag cctggtaggt ttgctttggt gagttctgga
    11581 aagcggcaag atcgcattca gcgcgacgat tccaggccgc tacccgttct tgtcgttgag
    11641 attgttcaat tttctgcgtc attttgattg ccttattctt cgcggatgcc ggaaagttct
    11701 gcacgggaga gcaccagacg ggcacgatcg ataaccggac cgtccaccat cttgccgttc
    11761 agggaaacca cgccgaggcc ttcgcgagcg gcggcttcag cggcttctac gacgcggcgg
    11821 gcgtgatcca cttctttctg ggtcggtgcg tagaggttgt gcaacagatc aatctgacgc
    11881 gggttgatca gcgatttgcc gtcaaagccc agctgtttga tgtgggcggc ttcttgcagg
    11941 aatccggctt cgttgttagc gtcggaatag acggtatcga acgcctgaat acccgcagag
    12001 cgcgcggcct gcaaaatgga acagcgtgcg aacagcagtt cagttccttc cggggagcgt
    12061 tctgtacgca ggttgcgcac atagtcttct gcaccgaggg cgataccgat caaacgctcg
    12121 gaagcgtgag cgatttccac tgcgcgggta atccccagcg gagattcaat cgccgccagc
    12181 aggccggtgc tgccgggttc acgaccacag gctttttcga tacgcaggat ctctttttcg
    12241 atatccagaa catcctgagc ggtatcggtt ttcggcagac gcacaacgtc cgcaccaccg
    12301 cgaacgacgg cttccaggtc gttgacaccc cattcggaat ccagcgcgtt gacacgcaca
    12361 atggtttcaa tatcgcgata cagcggatgt tgcagcgcgt ggtaaaccat gcggcgggcg
    12421 gtgtcttttt cacgcaatgc tacggagtct tcgaggtcaa acatcagggc atcagccggg
    12481 tagatgaagg agttgctgac catcgcggca ttggcaccag gcacaaacaa catgctgcgg
    12541 cgggtgcgag ttttacgttg ttgcagcgaa gcggaaatca ttggcaatcc tcccatggca
    12601 gagccgggat accgctggcg cgtgccagca gggcttccag tcgtgcacgt aaaatgcagt
    12661 ccagtgcgcc tttgtcatcg acattcagct gtacgccgcg cacgttgtag cgggcgagaa
    12721 cgtccagaat ggtggtgcga attgcatcgc caaactgttt ctcaacgctg ctattgattt
    12781 gcaggtcgat atcctgcgta tcgagtgggg cgatgcgtat catcacatcc ccagactcaa
    12841 gggtgcctgc aacggcgggc tggtttattt tcatttttca cctgtttctc atgcgggggt
    12901 cttttgacga gctgccgcgt cctggcggga gtgctcaagc aggttctgca aataatgcag
    12961 cgtgactgca gggaccagcg gcgcgatagc cgtgagatcg tttttcgcca gcagttgacg
    13021 tacccgggaa gcggatatcg gcatctcctg gtaacgcagc cgctcaattt caaccagttc
    13081 gatgggcggt gcggagatag tcggcgtttc cagccagtag cgcatatcct ggttgtactg
    13141 ggcggtaacg cgacaaaagg gttcagtacc gacaaagcgg tgagttacac ccagcgcggg
    13201 agcgaggtac tgacggaaaa tcttcagatc aatttcggtg taacaatggt taatgacgct
    13261 ctgttcttta atgaagtagc aagggaacgt agcgcgggag atgatgtatt cggagccgcg
    13321 atgcacagtc aggcgtggaa tatcggcggt gccttttaac accagatcca accggtcttc
    13381 ataggggaag cgtgaagaat cttctttgac taaaaacaga tgcaaccagt cgcactgtgc
    13441 cgcagcttgt tgaatcagat aacggtgacc attcgtgaag ggattggcgt tcatcacaat
    13501 gcagccaatc ttgttccctg gatgacgaaa ttttttcagc gattcggcat agcgtttcag
    13561 ccgcgtggcg ctgttttcca tcagcaccat cacgccgggt acgctggtca gcgtggaaaa
    13621 accgcactgg cggaacagcg cctcgtattc ggttttggta taaataaaca gatgcgtgct
    13681 gtgccgctca taggcgaggt ttatcaattc agtggctaat gtcagcgcca gtccttcacc
    13741 gcgaacggat tcactgatag caacgcattt aataatattt ccggcaattc caccgcacgt
    13801 gataagcttt tcatcgcggg ttacggtaat aaatacttcg actgtggtgt caacg
//
LOCUS       DAZPLI010000107        13551 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZPLI010000107 DAZPLI010000000
VERSION     DAZPLI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13551
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.108"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            46..1812
                     /gene="adeD"
                     /locus_tag="ACHUU3_002967"
     CDS             46..1812
                     /gene="adeD"
                     /locus_tag="ACHUU3_002967"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR2082979.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(1857..3248)
                     /gene="uhpT"
                     /locus_tag="ACHUU3_002968"
     CDS             complement(1857..3248)
                     /gene="uhpT"
                     /locus_tag="ACHUU3_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR2082980.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3386..4705)
                     /gene="uhpC"
                     /locus_tag="ACHUU3_002969"
     CDS             complement(3386..4705)
                     /gene="uhpC"
                     /locus_tag="ACHUU3_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR2082981.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4715..6217)
                     /gene="uhpB"
                     /locus_tag="ACHUU3_002970"
     CDS             complement(4715..6217)
                     /gene="uhpB"
                     /locus_tag="ACHUU3_002970"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR2082982.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6217..6807)
                     /gene="uhpA"
                     /locus_tag="ACHUU3_002971"
     CDS             complement(6217..6807)
                     /gene="uhpA"
                     /locus_tag="ACHUU3_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR2082983.1"
                     /translation="MITVALIDDHLIVRSGFARLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6882..7172)
                     /gene="ilvN"
                     /locus_tag="ACHUU3_002972"
     CDS             complement(6882..7172)
                     /gene="ilvN"
                     /locus_tag="ACHUU3_002972"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR2082984.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7176..8864)
                     /gene="ilvB"
                     /locus_tag="ACHUU3_002973"
     CDS             complement(7176..8864)
                     /gene="ilvB"
                     /locus_tag="ACHUU3_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR2082985.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVEE"
     gene            complement(8970..9068)
                     /gene="ivbL"
                     /locus_tag="ACHUU3_002974"
     CDS             complement(8970..9068)
                     /gene="ivbL"
                     /locus_tag="ACHUU3_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR2082986.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9633..9722
                     /gene="tisB"
                     /locus_tag="ACHUU3_002975"
     CDS             9633..9722
                     /gene="tisB"
                     /locus_tag="ACHUU3_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR2082987.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9846..9920)
                     /gene="ysdE"
                     /locus_tag="ACHUU3_002976"
     CDS             complement(9846..9920)
                     /gene="ysdE"
                     /locus_tag="ACHUU3_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR2082988.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            10002..11186
                     /gene="emrD"
                     /locus_tag="ACHUU3_002977"
     CDS             10002..11186
                     /gene="emrD"
                     /locus_tag="ACHUU3_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR2082989.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11194..11691)
                     /locus_tag="ACHUU3_002978"
     CDS             complement(11194..11691)
                     /locus_tag="ACHUU3_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR2082990.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11688..12050)
                     /locus_tag="ACHUU3_002979"
     CDS             complement(11688..12050)
                     /locus_tag="ACHUU3_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR2082991.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12040..12387)
                     /locus_tag="ACHUU3_002980"
     CDS             complement(12040..12387)
                     /locus_tag="ACHUU3_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR2082992.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12496..12945
                     /gene="yidI"
                     /locus_tag="ACHUU3_002981"
     CDS             12496..12945
                     /gene="yidI"
                     /locus_tag="ACHUU3_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR2082993.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(12992..13150)
                     /locus_tag="ACHUU3_002982"
                     /pseudo
     CDS             complement(12992..13150)
                     /locus_tag="ACHUU3_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13194..>13551)
                     /locus_tag="ACHUU3_002983"
     CDS             complement(13194..>13551)
                     /locus_tag="ACHUU3_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR2082994.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPA
                     YTSQRCVCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQS
                     GRSLKQEPTEMIQATA"
BASE COUNT         3207 a         3776 c         3537 g         3031 t
ORIGIN      
        1 tatgataaat aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattctatt
       61 aaccataaat ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt
      121 ggcgacgctg ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatggc
      181 ggagaaattt ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa
      241 tacgctgatg ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg
      301 tttattgatg ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc
      361 gctaccctgc cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg
      421 atgggagaag ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag
      481 tacttacagg tcagctcttg cgtacccgcc ctggaaggct gcgatgttaa cggtgccagt
      541 tttacccttg aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa
      601 atgatggact accctggcgt aattagcggg cagaatgcgc tgctcgataa actggatgca
      661 tttcgccacc tgacgctgga cggtcactgc ccgggtttgg gtggtaaaga acttaacgcc
      721 tatattactg cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg
      781 aaattacaac tcggcatgtc gttgatgatc cgcgaagggt ccgctgcccg caatctcaac
      841 gcgctggcac cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac
      901 cgtaacccgt gggagatcgc ccatgaagga cacatcgatg ccttaattcg ccgcctgatc
      961 gaacaacaca atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc
     1021 cactttggtc tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg
     1081 ttgagcgatg cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga gccgattgat
     1141 gcgcaaacct tacaggcgga agagtcggcg agactggcac aatccgctcc gccatatggc
     1201 aacaccattg cccgccagcc agtttccgcc agcgactttg ccctgcaatt tacgcccgga
     1261 aaacgctatc gggtcattga cgtcatccat aacgaattga ttacgcactc ccactccagc
     1321 gtctacagcg aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt
     1381 tacgggcaac ggctggctcc ggcttgtggt ttgcttggcg gctttggact gaatgaaggt
     1441 gcgctggctg cgacggtcag ccatgacagc cataatattg tggtgatcgg tcgcagtgcc
     1501 gaagagatgg cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta
     1561 cgtaacggcc aggtccaaag tcatctgccg ttgcccattg ccggcctgat gagcaccgac
     1621 acggcgcagt cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt
     1681 ccgttacccg atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg
     1741 ctaaaactaa ccagtcaggg gctatttgat gtcgagaagt ttgccttcac tacgctggaa
     1801 gtcacggaat aataaaaaag cccggcgtca tgccgggcaa aagtcaccag ttacgtttat
     1861 gccactgtca actgctgaat ttttttctcg cggcggattt tgcgttcttc catcaccgcc
     1921 actatcgcca tcaggcagat acaaccaatc gcggcgatat caagtgcggc gaaggtgcct
     1981 gcccagccgg taaggccgaa taccggcgtc ccatcggcaa tcattcccag acctaacttg
     2041 gcaaagctgt caccaatcag gtaagcaaag gtgcctttaa taccatcggc agcgccaatc
     2101 gcttttttag gtacaaagcc aacagcagcc acaccaatca acaattgcgg gccaaagacc
     2161 aggaaaccca acgcaaagag agaagccaga tagatatatt cgttactggc atgttgatac
     2221 acaccgagcg tggcgataat cagcgccagc gcgatgcagg ccaccaggcc acggcgaccg
     2281 ttcgctaggt cagagagcca gccccacagc agcgtaccga ccagcgcacc agcttcaaac
     2341 agcgtaaagc cctgaatcgc caccgcttta gagagtttca gttcctggaa cgcgtatacg
     2401 gttgaccact ggtcgatacc aatacgtacc acatagagga aaatgttggc gaagcacagc
     2461 agccagatca ctttgttttt cagcacatac tcaacaaaga tctgccactt ggtcatatcg
     2521 gtagattctg tctctttgtc ctcttcgctg atctcctcgc cgaacagttc ttcagctttg
     2581 ccgaggccat aagattccgg ggagtcgctg ccgtaacgca ggccgataaa accgacaatc
     2641 agcgcgataa tcgacgggaa gataaacatg ccgatgacat ggccatcgaa caggtaattt
     2701 gccccgaaca gcgccacgcc tgctgcgcct gcaccgccaa ggttgtgaga aatattccag
     2761 aaaccgagga atgtcccgcg tttacgacgc ggcgtccatt tggtgatggt ggagtaactg
     2821 cacgaaccgc cggtactctg gaaaaagccg cttaaggcgt agaaggcaat catcaggaac
     2881 aggctaaccg agccgctgcc catactggca ctgaagccca gcatacaaat ggcagacagg
     2941 atcagcatga acggcaggaa ttgtttggtg tttttgccgt cggcgtagta ggaaaccagc
     3001 gttttaccca cgccataagt gatggagaaa cccaggccga tcatccccag ctgcgtcatg
     3061 ctcaacccgt aggtcgagat catgtcgttc tgcgcgatgt taaagttctt gcgaatcagg
     3121 tacatcgtca ggtagccgat aaagaccacc aggtaggatt gcatgaacgg tttgaaccac
     3181 attttgcgcc gcacttcgag cggaaggtcc agggtcggct tgcgaacctg gtttaagaaa
     3241 gccagcatgg gttactcctg aaatgaatac ctgcgtgagg catgcattgt aaaaatcagc
     3301 agcaaatcag gcctgagata gcgtccaggc aaaacctgga aaatttctta gttttgccgg
     3361 atatgaagtg aaaaggtgag atgcatcacg cttcgcgcgg tgtctgggcg ttcaaaaagg
     3421 gcagtaacag cagtgcggaa atcccggcgg cgatagagat aaccacaaaa aatccgctcc
     3481 agtgccaggt atcgagtact ttcgccagcg gccaaccagc aagcgacgcc cccagataag
     3541 caaacaagcc gacaaacccc gtcgccgccc ctgccgcctc tttgtgggaa cactctgccg
     3601 ccgccatacc gattaacatc tgtgggccaa agacaaaaaa accaatggtg aagaagcagg
     3661 ttgcctgcat cacgtagctg gcaaatggca tcagccacag ggagccgact gaaagcaaaa
     3721 ttccggcggc gaaaatcaaa ttcatcggcc ctcggttgcc gttaaacaat ttgtccgagc
     3781 cccaacccgc taccagcgca ccgataaatc cacccagttc aaacatcgtc actgccgtat
     3841 tcgccgtgac cagatcgacg cccagcgtct cggacatata caaattgccc cagtcgttga
     3901 tcgccgcccg gaccacatag accagcacat agcaaaacga aagcagccag atatacggat
     3961 tcagcaacac atatttggtg aggatctctt tacgcgtcag ccctgcccct tcttgttgtt
     4021 gagctatctc cagcgcgtcg tgtcgccatt caccgaccgc cggtaaacct aacgcctgcg
     4081 ggcgatcgcg cagccgccag cagagaaaaa ttcccacgac gatcgccata caaccagcaa
     4141 tcatcatccc ggcacgccag ccgtaatgta gtgcagccgc tgccatcaca atgggaatga
     4201 gtgcgccgcc gacgttatgc gccgtgttcc ataatgccca ccaaccaccg cgctcggtac
     4261 gtgaatacca ggccgttaac agacgcgcac acaccggtga accccagccc tggaaaaagg
     4321 cgttcagcac ccagagtacg gcaaacgccc atagcgacgt cgagaagcca aacagaatgt
     4381 tgataatgcc cgtggcgata agccctatcc ccataaaata acgggcattt gagcgatcgc
     4441 tgacaatgcc ggagacaaac ttcgacacgc cataggtaat gtaaaacagg gtcgctaaca
     4501 ggccgatatc gctacggctg agcacgccgt tagcaaggat ttctggtacg gcggcgttaa
     4561 aacttttccg cgtgaagtaa aacagcgcgt aacccagcca gatggtcagc agaatatgcc
     4621 gacgccagta gcgatagcgg gcatcaattt catatttatc agccattaat ggcgcatcgg
     4681 caggcgcttt cagaaacggc aacatcatca aaccttagac atagcgttga ggtagagaaa
     4741 cgctgacacg cgtgccgtgc agacaggaaa tgtgtaatgt gccacccagc gccgttacgc
     4801 gctcgcgcat tccggtgagg ccaaaacctt gttgcccgga acccggcggc aaaccgctgc
     4861 catcgtcttc aataaccagc atcaaccgtt catcctgctg ccagccttgc agggtgaccg
     4921 cgctggcatc agcatgtttc acaatgttgt tcagcccttc ctggcagaca cgaaacagcg
     4981 tcacgcgctg gttttcgctt aacgctgatt catcgattcg ccattcgaga tggctgacaa
     5041 taccgcgccc ttccagctcc atttcccgca tcagtgagcg gatggcctgc tccagggtga
     5101 gatcatccaa ctggcgcgga cgtaaccgac ccaacaaacg gcgcaccgcg tcgtaaacgc
     5161 ccagcgatag ttgttcgatg agttgcccgc tctgcttcac gctggcgtta tctgccgcca
     5221 gccgctgaac aatgcccgcc tgagtacgaa tagcagtgat ggtctggccg atatcatcat
     5281 gcagctcacg cgccacatca cggcgcacgc tctcttcggt ttccagcaac cgttcagcca
     5341 gatgctgatt gcgcgccagt tccttttgca gcgactggtt aagttcacgc aaccgctgga
     5401 tgccagcgcc aagcaacaac cctgtcagac tttgcaccag cagcgagagc aataaatcca
     5461 ccggatgatc gcgccaggtt tgactggcga tcagcgcgat ggcgttcatc aacgtcgcaa
     5521 tcagcgcccc ttgccaacca tagtgccagg ccagcgcgat aatcggcagc accagacaga
     5581 atggcgtaaa gcgcgacagt tcgtccggca atcccaactg gagccagaga ctgataacaa
     5641 acagcagcaa gtaccagacc agatgtcgcc cgcgccagtt gattggctga gaaaccagtg
     5701 acgggccgag cggcagccag gtgttattgg cgagatagtg ccagaacacc agacatatcg
     5761 gggccagcgt caggccgcca gttaaagtca gcaacagcgc attccacgac tctttgccgt
     5821 gccaaagcca gggcagcgac tgcaacaacg ccgccgccgt taacgccgcc ccctgtaaca
     5881 gcaaggtgcg ccagtcacgc tgatggcgat agcgcgagat cagcgctacg ggcagtaacg
     5941 tcagtaaact accgatcatc aataacggaa aatgggttaa accgaccgcc tgcgttagcc
     6001 agtaaatcag cagccactcc gcacccagca atacgggcca gtatccgcgc gggcattgca
     6061 gcattagccc cagacgcaga ccaaacggaa ataacagcac cgccatatca gggcgctcaa
     6121 ccagatgcag gctgatactc cacaggcaaa accatgcggc agagaagata aaaaagcagg
     6181 caataacggt aattaagcgg gagaacaacg tcttcatcac cagccatcaa acatgcggcg
     6241 cgccagctct acgtcgttac tgacgcccag tttttccatc agattggcgc gatggacgtg
     6301 taccgttttc ggtgacaagc ccagttcggc ggcaatctct ttcaccgcca ttccttgcgc
     6361 cagtttttcc gccacctggc gttcacgttt ggttagcggg tcctgacgac cggatgccag
     6421 tttaatggca atatccggcg tcagataaca gccacccgtg gcaaccgtat gcaccgcagc
     6481 aatgagttca tccgggctac agcgtttgga aagaaagccg cgtgcccccg cgttaagcgc
     6541 ctgctcaacc agcgcaggac tgtcgtgaac ggagagcata atcgtcgcca tacctttcgg
     6601 cagctggctt agcagctcca gaccggagat atcgggcatg gagatatcgc aaatacacac
     6661 ctgcacaccg cgccccggca gccccgccag cgcctcgcgc cccgaaccaa actcggcaac
     6721 tacctgcaaa tcaggttcca gccccagcag ccgcgcaaag ccggagcgga cgatgaggtg
     6781 atcgtctata agggcaacgg tgatcatggt cttgtcctgg cgggtaaaaa aaaacgcgct
     6841 taccttaacg ataagcgcga tgttgttcaa gccttgagcg gttactgaaa aaacaccgcg
     6901 atcttgttaa acatcgtcgg atcggactga ttacgctgca ctttcacgac atcttccagc
     6961 ttatcgattt ggcttatcat ctgctccaga cgctggtcgt cattgaccag tagccagata
     7021 tggcttttgt cgctgtcctg aatcggcaga caaagaatgc cttcaacgtt aaaagcgcgg
     7081 cgggcaaaaa ggccacaaac gtgggtcatt acgcccggat ggttgcgaac ggtgagctcc
     7141 agaattacgt tgtcatgagt tgtgttttgc atggcttatt cctccaccat ttcagtattc
     7201 gccgcacctg gcggcaccat cgggtaaact ttttcttcgg catcaatgcg cacatggatc
     7261 agcgccgggc tagggcgatt gatgatttcc tgcaatgcag cctgcggatc ggcttcgtta
     7321 ttcaaatcac aggtttcgag gccgaatccg gcggcaatct gcataaagtt gattttgccc
     7381 ggataggtgg cggcaaaaac gccttgctcg tagaacagac tctgttgctg atgcaccagc
     7441 cccagcgctt cgttgttcat cagaatgatt ttgacatcca gctgattttc actggcggtc
     7501 gccatctcct gaatattcat catcaggctg ccgtcgccgg agaaacacaa cactttgcga
     7561 tccgggttcg ccagcgccgc gccaatcgcc gcaggcaggc caaaacccat cgtgcccagc
     7621 ccaccggagg tcagccactg gcgtgggcga ttgagcggat aagcttgcgc ggtccacatc
     7681 tgatgctgac ccacatcggt ggtgataatc gcattgtcat cgacacaggc ggcaacggcg
     7741 ttgatcaggc cgtaatggct taatggatcg cacgctttcg ggattggaca cgggaattcg
     7801 cgctgcaaat ccgctaccaa ctggtgccac tctgcacgcg gttgcgcttc cacctgcggg
     7861 atcaattgcg ccagcacgtc atcaacatcc gcctgaatcg ccacatgcgg ctgcttgatt
     7921 ttacccagct ctgcgcgatc gatatcgaca tgaataattt tggcattcgg acagaactgc
     7981 tcggttttgc caatcgcccg gtcatcaaaa cgcgcaccga gcacaatcaa caaatccgcc
     8041 tcctgcaaaa tatagttggt actgcgcacg ccgtgcatcc ccagcatacc cagcgacaac
     8101 ggatgcgctt ttggcagcat gcccagcgcc attaaagtca tggtggtagg cagttgcgct
     8161 ttctccgcca gttcacgcac ccgcgcaggc gcattgatca caccaccacc cagataaagc
     8221 accgggcgtt tggcagcgtt aatcattgta gctacgtcac gaatgctttc ttcgctaaag
     8281 gcgggtgcag cggctttttc tgccaccgcg ggctgagctt caatctcaaa aaccgccgtt
     8341 tgtacatcct taggaatgtc tatccacacc gggccagggc ggcctgattg cgcaatgcgg
     8401 aaggcatcgc tcatgacctg cgggagttct tcgatatgtc tgaccagata gttgtgtttg
     8461 gtgatgggga tagagatgcc gtaggtgtcg acttcctgga aggcgtcggt gccgatcatc
     8521 gaggcgggaa cctgaccagt gatgcaaatc agcgggatgg agtccagccg cgcatcggca
     8581 atggcggtca ccaggttagt cgcacccggt ccgctacagg ccatacagac cgccggttta
     8641 ccgtcggtgc gcgccattcc ctgagcgata aagcccgcgc cctgttcatg acgggccaga
     8701 atatggcgga tttgcgtgct ttggcttaag gcatcgtaaa caggcaggat agaaccgccc
     8761 ggaatgcctg tcacaatctt aatgccctgc tgttccagga aatgaacgat aaattctgcg
     8821 ccggtaaagc gcttacgcgt caatgttgtg cccgaacttg ccatgctcca gtccttttct
     8881 tctgggccga ctttccggga ggtgcttaaa cgaaaaaccc cgcccggttt gcgccgacgg
     8941 ggttttggaa tcgtgtgttg ttccagtccc tacggcgcat tgccgacgac caccaccaca
     9001 cgcacgacga ccactgcggc ggatggcgca gttggtagta gttttgcgtt gaacatggaa
     9061 gtagtcattg gggaccttgt tggttttgtg tttaacaata tttatacaag cacagcttta
     9121 caggggagac aatggaaaat ttttcagcaa gggaaaattg aggggttgat cacgttttgt
     9181 actgaattgc agataacaaa aaaccccgcc ggagcgaggt ttcgtcagtc gcccgcggct
     9241 ggtaaccgca aagcacactg tattatgtca acacagaaag tatacgtgtt ccgcgcagaa
     9301 cgcgcaattt cggcacgaat tttgacgtat ttagtgcata gttgagtatc gatcacagtt
     9361 tgcgttttgt ccaaatatta ctgtttattt atacagtaaa cttctataat atcactgtac
     9421 gcaatgtgtt atgcgggggc cgcatcgtta cccggcgcac taagtcctgg ctgaaacggg
     9481 tggtgccgtc agcgccttaa ccccgcgtga gcacactgtg ttatgtcaac aagcacaaag
     9541 tttctccttg agataccgcg tgcacaacag ctggcaacag gcagcggaaa ggtacgtcag
     9601 ctggcagtgc tcctgaacca caggagacgc gtatgagcct ggtggatatc gccattctta
     9661 tcctcaaact cattgttgca gcactgcaac tgcttgatgc tgttctgaaa tacctgaagt
     9721 aattcagatt caagtcgcac caaaggggag cgggtaaccg ctcccctttt atatttagcg
     9781 tgcgggttgg tgtcggatgc gatgctgacg tatcttatcc gccctaccat ctctcccggc
     9841 aacatttatt gccgcttttg tttacatatt ctgccgctaa acaactcccc attcctggcg
     9901 tatatctggc taacattcat caatgtgata gattcctctc ccgcatttat gggaatgcgt
     9961 agtgacttac tctaattatt tttataaaag catccgtgat aatgaaaagg catagaaacg
    10021 tcaatttgtt attgatgttg gtattactcg tggccgtcgg tcagatggcg caaaccattt
    10081 atattccagc tattgccgat atggcgcgcg atctcaacgt ccgtgaaggg gcggtgcaga
    10141 gcgtaatggg cgcttatctg ctgacttacg gtgtctcaca gctgttttat ggcccgattt
    10201 ccgaccgtgt gggtcgccga ccggtgatcc tcgtcggaat gtccattttt atgctggcaa
    10261 cgctggtcgc ggtcacgacc tccagtttga cggtattgat tgccgccagc gcgatgcagg
    10321 ggatgggcac cggcgttggc ggcgtaatgg cgcgtacttt gccgcgagat ttatatgaac
    10381 ggacacagtt gcgccatgct aacagcctgt taaacatggg gattctcgtc agtccgttgc
    10441 tcgcaccgct aatcggcggt ctgctggata cgatgtggaa ctggcgcgcc tgttatctct
    10501 ttttgttggt tctttgtgct ggtgtgacct tcagtatggc ccgctggatg ccggaaacgc
    10561 gtccggtcga tgcaccgcgc acgcgcctgc ttaccagtta taaaacgctt ttcggtaaca
    10621 gcggttttaa ctgttatttg ctgatgctga ttggcggtct ggccgggatt gccgcctttg
    10681 aagcctgctc cggcgtgctg atgggcgcgg tgttagggct gagcagtatg acggtcagta
    10741 ttttgtttat tctgccgatt ccggcagcgt tttttggcgc atggtttgcc ggacgtccca
    10801 ataaacgctt ctccacgtta atgtggcagt cggttatctg ctgcctgctg gctggcttgc
    10861 tgatgtggat ccccgactgg tttggcgtga tgaatgtctg gacgctgctc gttcccgccg
    10921 cgctgttctt tttcggtgcc gggatgctgt ttcctctggc gaccagcggc gcgatggagc
    10981 cgttcccctt cctggcgggc acggctggcg cgctggtcgg cggtctgcaa aacattggtt
    11041 ccggcgtgct ggcgtcgctc tctgcgatgt tgccgcaaac cggtcagggc agcctggggt
    11101 tgttgatgac cttaatggga ttgttgatcg tgctgtgctg gctaccgctg gcgacgcgga
    11161 tgtcgcatca ggggcagccc gtttaagcgc acgtcaccgc agcatcgtca tcagctccat
    11221 gggagaacga tgctgcttta tcagatcacg catcacccgc atatgcggtg cggagtaaga
    11281 ataaaacgcc tgatagcccg cacaaagcac gctgtctggc gtctcctgcc gagcgtgcgg
    11341 gcacaggcga tgccagtcgc acgcctggca gtcacagcgc ggttgcgggt tttcgctgcc
    11401 acaccatttt tgtaaggtgg cgtcaaaaag ctcaatcgag atacgactga tatctgcccg
    11461 cacccaacac tcgaacaggg caattaaaaa tttcccccac agctctgctg tcaccgaagc
    11521 ggccgtcaga tcgccccggt catcaaattc aagataggga atgagctgta cggagcggat
    11581 gccccgctgt ttgaaattgt gataaaaatc ggcaggttgc agagcatcct tgtactctac
    11641 aaccagttta tgcctgtcct cttcagcatc aatgacctga cttcctgtca tgcgactctc
    11701 tcaataaata cgataagttg atgtatatgc gttacagcaa acagtattgc gagagataac
    11761 accgcgaggg agatcaaaaa tttgtcacgg actacgtgaa attgggaaaa atcgctattc
    11821 gtgacatcca ttaaattgcg attacgggtg tagtgccaga ggatcagcgc cacgatggcg
    11881 agaatgccaa tcgaaatcca gaataacatg cccgcctggt gccagttgtg tttgatagcc
    11941 aacgccatca acgcgccgta gcccagcatg gtacgaaacc acgccagtga tgtgcgctcc
    12001 ggctgcagtc ctgggtcggc aatgcgtctg gctttgcggc tatccggcat acaacaccag
    12061 tcccataacg atcaccgcca cgaccatcaa tattaagcta atgattaata agctgttggt
    12121 gtagggcagg tcctctttca gacgcatcgc tttttcattg cgtaaccagc gcaggtagcc
    12181 atacattgct aacccgccgg aaaacagaca caacaacaac gccagcagtt cacgaatgac
    12241 gggcgtggcg aaatctggcg caagttgatc aagcccaacc cccgccgcca gaaatcctag
    12301 tgcggtacgg atccacgcca gaaaagtacg ctcattagcc agcgagaagc ggtaatcggg
    12361 cgcttctcca aggcgggaaa ttttcattgt tgctcctttg caatttcact aactctctac
    12421 ctttagcggt tttttttaag aatttcgtcc gcccgtttcc tgatatgctt ttgccatccg
    12481 ctacaaggag gtcagatggg tatcatagcg caaaataaaa tcagttcatt gggcatgctg
    12541 ttcggtgcca tcgctttaat gatggggatt atccactttt cattcggccc tttttccgca
    12601 ccaccgccaa cgcttgaaag catcgtggcg gacaaaacgg ctgaaattaa aagaggtctt
    12661 ctcgccggaa taaaaggcga aaaaattacc accgtggaga aaaaagagga cgtagatgtc
    12721 gataaaatcc tcgatcaaag cggtatcgca ctggccattg ctgcgctact ttgcgcattt
    12781 gttggcggta tgcgtaagga aaatcgctgg ggcattcgcg gcgcgctggt ttttggcggc
    12841 ggaacgctgg ctttccatac cttgcttttt ggtatcggca tcgtgtgcag cattttgctg
    12901 atattcctta tcttttcctt cctgaccggc ggttcgctgg tttaagcagc ctgaataatg
    12961 cccgccgggt gatcatcagc gggcattatc gtcagaactt ctgttttttc tcttccacct
    13021 tcaccccttg tgtcggtagg ccggtgtcat agtcacgtac caccggcgaa tagccatctt
    13081 gtgggcgtgg acgaaatgcc cccatccagc gcggccgtgc atctttacgc cacggacgca
    13141 ggctccattg ttgacatcct ccacgccctg aaggacgtgg acccctgcta cgttcaggct
    13201 gtcgcctgaa tcatttcggt gggttcctgc ttcaacgagc ggcctgactg caccatctct
    13261 ccacaggcaa gaacggcgtg ccccgccgct aaaatgttac gagcgccatt tacatcggcg
    13321 ttcgctgtat atccacatac ctggcatctg aatttacttt gtgacaggcg attttctttc
    13381 gctgtatgac cacagcacac gcaacgctgg cttgtgtacg ctggcggaac agcaagcacc
    13441 tgaccaccac gccagagctg cttgtacgca agctggcggc gcatttcata ccagccctga
    13501 tccagtatcg aacggtttaa acctgatttt gcccggacat tgcgccccgg c
//
LOCUS       DAZPLI010000108        13531 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZPLI010000108 DAZPLI010000000
VERSION     DAZPLI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13531
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.109"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(84..806)
                     /gene="baeR"
                     /locus_tag="ACHUU3_002984"
     CDS             complement(84..806)
                     /gene="baeR"
                     /locus_tag="ACHUU3_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR2082995.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(803..2206)
                     /gene="baeS"
                     /locus_tag="ACHUU3_002985"
     CDS             complement(803..2206)
                     /gene="baeS"
                     /locus_tag="ACHUU3_002985"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR2082996.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(2203..3618)
                     /locus_tag="ACHUU3_002986"
     CDS             complement(2203..3618)
                     /locus_tag="ACHUU3_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2082997.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(3619..6696)
                     /gene="mdtC"
                     /locus_tag="ACHUU3_002987"
     CDS             complement(3619..6696)
                     /gene="mdtC"
                     /locus_tag="ACHUU3_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR2082998.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(6697..9819)
                     /gene="mdtB"
                     /locus_tag="ACHUU3_002988"
     CDS             complement(6697..9819)
                     /gene="mdtB"
                     /locus_tag="ACHUU3_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFR2082999.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(9819..11060)
                     /gene="mdtA"
                     /locus_tag="ACHUU3_002989"
     CDS             complement(9819..11060)
                     /gene="mdtA"
                     /locus_tag="ACHUU3_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFR2083000.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            11339..11395
                     /locus_tag="ACHUU3_002990"
     CDS             11339..11395
                     /locus_tag="ACHUU3_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR2083001.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            11666..11722
                     /locus_tag="ACHUU3_002991"
     CDS             11666..11722
                     /locus_tag="ACHUU3_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR2083002.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            11995..12054
                     /gene="ibsA"
                     /locus_tag="ACHUU3_002992"
     CDS             11995..12054
                     /gene="ibsA"
                     /locus_tag="ACHUU3_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFR2083003.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            12275..12934
                     /locus_tag="ACHUU3_002993"
     CDS             12275..12934
                     /locus_tag="ACHUU3_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR2083004.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            12931..>13531
                     /locus_tag="ACHUU3_002994"
     CDS             12931..>13531
                     /locus_tag="ACHUU3_002994"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFR2083005.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSP"
BASE COUNT         3165 a         3714 c         3679 g         2973 t
ORIGIN      
        1 tcagtttgct gaccatgcgt gggataatgt tgccggatct ggtctgaacg ccatctccgg
       61 ctaacaaagt catgtcgcta aaactaaacg atgcggcagg cgtcggcttc ccagcggtaa
      121 ccaacgccgt aaacggcgcg gataaatgac tgttcggcgt cgagagattc cagcttgcgg
      181 cgcaggtttt taatgtggct gtcaatggtg cggtcggtta ctacgcggta gtcgtcataa
      241 agatgattga gcaattgctc gcgggagaac acttttcctg gttcatgaga gagcgttttc
      301 aacagacgaa attccgcagg cgtcaggtca agcattttac cgcgccatga agcctgaaaa
      361 cgaccttcgt cgataatcaa cgggctttcg gcatcctgtt gctgcaattc gcgctgcggt
      421 ttgcaacggc gcaaaatggt tttgacgcgc gctaccactt cccgtgggct gtacggcttg
      481 cagatgtagt catctgcgcc aatctccagt ccgagcaggc ggtcgatctc ttcgattttt
      541 gccgtcacca tcacgatagg aatgtcagaa aaacgacgaa tttcccggca cagcgtcaag
      601 ccatcggtgc cagggagcat cagatccaac aggatcagat ccggtggtgt ctggcgcaca
      661 tacggcagta cctgatcgcc gtggctgata agcgtcggcg catagctcgc agcacgcaga
      721 taatcaatga gcaactgccc cagcttcggt tcatcttcca cgatcaaaat acgcggtgtg
      781 ttttcgtcga ttggtaactc ggtcatactt ctctctgtaa atcccgttcc agcggtaact
      841 ctactgtaat gcttaccccg ccaaaaggcg aatgggcagc aataatgcgg ccattatgtg
      901 cttcaacaat gttcaggcaa atcgccagcc ccagcccgga accaccgctg gcgcggttgc
      961 gggaaccttc ggtgcgataa aaacgttcaa ataatttttg tagctgctcg tcactgacac
     1021 ctggcgcact gtcggcaaag gtcaggcgca ccgttttgtc gtgctgctcg gcagagattt
     1081 tcaggctgcc gccgctgtca gtgtagcgca ggctgttttc aagtaaatta ttgaataact
     1141 gcattaaacg gtcgcgatcg ccaaatacgg taatactgtc tggcagggaa aattgcagtt
     1201 tcaggccacg gctggcgaat cgttcgcgaa atgcgccgcc cgccacttcc agcagtggga
     1261 tcaaatctac cggtgctttt tgataggcga gggcgccttc atcagacatc gacaactgat
     1321 ggagatcgtc aaccagtttg gtcagtgtac cgacctccgc ctgtaaagac gccaccgtct
     1381 ccggcgtgaa tttgcgcacg ccatcctgaa tagcttccag ttcaccgcgc agcaccgcta
     1441 atggcgtacg cagttcgtga gaaatatcgg ccataaaatc gcgccgcatt tgctggtttt
     1501 tctccagtgt gctggcaagc tggttgaagt cttgcgccag tttgcccagt tcatcttcac
     1561 tggtgggcgt tacgcgggta gtgaaatcgc ccgccgccag tttgtgcgtg ccatcgacaa
     1621 gtcgttttac cggtgccagt aaaccgcgcg ccagcagaaa agtggcaagt gccgcgagta
     1681 acgttgccag ggcgacaatc aaccagctgg tttgccgctg ttgtttatcg aaattgatat
     1741 cagtattgcg cgttaaccgc tcaacggggg aggcgatcac cgcgccaact tccgcaccgt
     1801 tgaccagaat gggtcgccgt gtaccgtcag gtggaatcgg cgctcgcgga ccaaccagca
     1861 ctttgttgtt ttgatcaacc acccagaact gggtacgcca gccgtgcggt ggcatacccg
     1921 ggccgggttt atcttccgaa ttatcgtgtt caaatgaacg caggatctga aagacaaagc
     1981 gatcattgtt gcgcaggaag cgccagttgc catgctgcgc atactgctcg ccaagcgcat
     2041 cacttaacag ttgtaatcgc tgttcattac catgcttgat gtaatcaata aagccacgct
     2101 caaaactgat acgcaccgcc cagtgcatac tgatcagcaa aacaatgcag gtggcgaaaa
     2161 tcgccagaaa cagtttgccg gtaataccag gtcgccagaa cttcattgcg cgctcctttt
     2221 tcgccgcgaa atagctacat tttgatgcgt atcgttcggc actctggcaa agatgaacgc
     2281 cggaagggcg atgataaacg ccatgctaag ccaggtgtac ataaagacgg tttgtgtggt
     2341 gccgctgtcg acgctgacat gctgcgaacc aaaaagtccc agcaacagcc cggcgatggt
     2401 gacgccgata ctcatcgaca attgcataat catcgacagc agactgttgc cgctgctcgc
     2461 cagattgtcc gggagatctt tcagcgtcag ggtgttcatg gaggagaaac gggtcgagtt
     2521 gaccatccct tgtaaaaaca ggacgaacgg caaaacgtag taccagccca acagagcggt
     2581 agtcataaac aacagggtga tcagcgacag acccagcgtg gtcgctacca gtacccgacg
     2641 ataaccaaag cgattcacca cctgaaccac aattcgcttc attcccatgc tgccaagtac
     2701 catcgggatc atcatcagtc cggcatgaaa cggcgagaaa ccgaggccaa tttgcaggaa
     2761 aaccggtgtc ataaagggca acatgccact gccaatacgt ccggcaaagc tccccgccag
     2821 gcccagcgaa aaggtacgag tgcggaacag tttcagactg aacagggcgc ggttgttatt
     2881 tctggcgtgc agcagataaa gcaccagtgc caccacgcca actgcgacca ggccagcaat
     2941 cgccagcggc gataaacctg tacctttact gccgtccagc gccagggtta atacagccat
     3001 gccaaccgcc agcaataaaa agccggaaag atcaaagcgc cgcgtctgca tggtgtagtt
     3061 cggcattaac agcaatgtgg cgatcgcacc gataatcccc accggaatgt tgatcaaaaa
     3121 gatccagtgc cacgacgcgt actccaccag cagaccgccg agcgccggac cgagcagcgg
     3181 accgacctga ccgggtaacg tgacaaaggt catcgccgcc atatattgct cgcgcggtac
     3241 gattttcatc accgtcaacc tgccgaccgg caccatcatc gcaccgccaa cgccctgtaa
     3301 cgcgcgtgcc agcaacagtt cgttcagcgt gccggaaagc gcgcaaaaca gtgaaccgag
     3361 agtaaacagc acgatggcgg taaagaaaat attgcgcacg ccgactttgt ccgccagcca
     3421 gccgctggcg ggcagcatca ccgccacggt cagcacataa gagacaatga ccatgtgcat
     3481 atgcaacgga ctttccccga ggctttgcgc cattgaggga agggcggtgt taacgatggt
     3541 ggtgtccagc gactgcataa agaagccgaa agccacaatc cacaattgcc agcgggtgct
     3601 gtcgggaagt tctgtcattt actcggttac cgtttgttta ggtttacgcg aaaaacgcag
     3661 ccgcagacgg tcgaaaaaga gatacaccac cggcgtggta tacagcgtca gaagctggct
     3721 cattaccagt ccaccgacaa tggtgatccc cagggggtgc cgcagctccg agccgtcacc
     3781 gcccgacaac accagcggca gcgcgccaaa cagcgccgcc agggtagtca tcataatcgg
     3841 gcgaaaacgc agcagacagg cctggaaaat agcttcctgc ggcgtcaggt taccgtgccg
     3901 ttgggcttcg agcgcaaaat cgaccatcat aatggcgttt ttctttacga tgccgattaa
     3961 tagcatgatc cctatcaggg ctattaggct gaacggggca ttgaacagct ccagcgccag
     4021 cagcgcacca acgcccgccg agggcagggt ggagagaatc gtcagtgggt gtacgtaact
     4081 ctcgtaaagg atacccagca cgatatacac cgtggcgatg gcggcgataa tcaggatcac
     4141 ctgtgagttc atcgtctcct ggaacacctg cgctgttccg gcaaaactgc cgcgcaccgt
     4201 cgaaggcaca ccaagctggg tcattgcgcg atcgatcgcc gcactggcgt ccgagagcga
     4261 ttttccggtc ggcaggttga acgagatggt cgaggccgcc gataatccct gatgattcac
     4321 cgacagcgga gcgttcgccg gttgccattt agcgaaatac gacagcggga tcgctttgcc
     4381 ttcgttattg ataacgaaca ttttttccag cgcactgatg tcctgggtat agcgcggatc
     4441 cacttccatc accactttat actggttcat cggctggtaa atggtcgaga tttgccgctg
     4501 accgaaggcg ttatttaaca ggctgttggc ggcctgcacg tcgattccca gccgtgccat
     4561 ggtgtcgcgg tcgtaaacca gattcatctc cgcgccgtta tcctgctgat cggagttcac
     4621 gtccgccagt tccggcaacg tcgccagttt tttgcggatt ttcggctccc attcgcgcag
     4681 tgccgccagg tcgtcggata acaacgtgta ctggtagctg gcgttcgact gacgcccacc
     4741 aacgcgaata tcctgtaccg ccatcaggaa cagattcgcc cccggttctt tcgccagttt
     4801 tacgcgcaga cggtcgataa tttgctgcgc cgtttcgctg cgttcgtcgc gtggcttgag
     4861 ggtgataaac atcatcccgc tgttcactcg cgaaccgcct gtaaagccgg tgacattatc
     4921 cactgccgga tcgtcacgga taattttcat gaaatcctgc aacttaccgc gcatcgcctg
     4981 aaacgaaata ctctgatccg cctgaatccc gcccatcaac acgccagtgt cctgctccgg
     5041 gaagaaggtt ttcgggatcg agatatacag ccagatattc agcgcaatgg tgccaagcag
     5101 caccacgccc accagacggg tatgattgag cacccatttc agtgacttgc cgtagccttg
     5161 ttgcagggct accaacatgc gaccaaaacc acgcagtcgc ttttgctcgc gcggcttgct
     5221 ggctttcagc atccagccac acatcattgg cgttaatgtc agagaaacca gcaacgaaat
     5281 accaatggcg acagaaagcg tcacggcaaa ctcgcgtaac agtcggcccg gcaatccgcc
     5341 catcaacagc agcggcagga acaccgccac cagtgacaga ctcatcgaca gcaccgtaaa
     5401 accgacttcg cgagtacctt gcagtgcggc ttgcaacggt ttcattcccg cttccagatg
     5461 acgtgcaatg ttttccagca ccacgatggc gtcatccacc acgaaaccag tagcgatggt
     5521 gagcgccatt aacgaaaggt tattgagact gaatccgcac aggtacatcg ccgcaaacgt
     5581 accaatcagc gaaaccggca ccgaaacggc gggaataata gtggcgcgac ccgagcgcag
     5641 gaataaaaac accaccagaa tcaccagcgc caccgagata atcagcgttt gctcgacttc
     5701 ttccagcgag gcgcgaatgg tgggggagcg atcctgggca atttgcagat caatcgccgc
     5761 cggaatggtt tcctgcaact ccggtaattt tgcccggatg ctgtcaaccg tctggataat
     5821 attggcttcc ggcagtttgc ggatcatcag taaaatagcc ggtttggcgt tggtcatccc
     5881 ggcgttgcgc acatcctgca ctgagtcggt caccgtcgcc acatcgccca gacgaaccgc
     5941 gccgccgttg ttgtagtgaa taatcaacgg ctgatattca gcggcggttt ttagctcatc
     6001 attggtctgg atctgccagc ggtgagtgcc atcttccagc gcgccctgcg gtttacgcac
     6061 gttggcattg ctgatggcgg tgcgtacgtc gtccagcgac acgccctgat taaacagcgc
     6121 ctgcggattc agcccgacgc gtacggcggg cagtgagctg cctccgacat cgacatcacc
     6181 aacaccgtcg atttgcgaaa tcgtcggagc cagctgcgtc gaggcgaaat cgtacagttc
     6241 accctgcgaa taagtatcgg atgtcagcgt gaggatcata attggcgcat ccgacgggtt
     6301 cgctttgcga taggtcggac ggctgggcat cccactgggc agcaaacttt gtgcagcgtt
     6361 gatcgccgcc tgcacatcac gcgctgcgcc gttgatgtcc cggtcaaaat caaactgcaa
     6421 aataatacgt gtgctgccga gcgaactgct ggaggtcatt tcgctgaccc cggcaatgcg
     6481 cccaagcgag cgctccagcg gcgtggcaac ggaagacgcc attgtttctg gtgacgcacc
     6541 gggcagcgag gcgctgacca taatcaccgg aaaatcgacc tgcggcagcg gggcgaccgg
     6601 cagcatacgg aagcccagta tgccgcacag ggtaatggcg accgacagta aaatcgtcgc
     6661 caccgggcgg taaatgaaga gggcaaaaaa cttcacttac gcctcctctt catggcgagc
     6721 aaagcgactt ttggtccaca atgccaggcg gtcgaacagc agataaatca ccggcgtggt
     6781 gaacagcgtc agcacctggc tgacaatcag accgccgacc atgccgatac ctaacggacg
     6841 acgcagttcc gcgccgaccc cggtactcaa catcagcggc agcgcgccaa gcagagccgc
     6901 cagggtggtc atcaggatcg gacgaaaacg caacagacaa gcctggtaga ttgcatcgcg
     6961 cggcgacatg ccttgctcgc gctcagcagc cagcgcgaag tcgatcatca tgatggcgtt
     7021 cttcttcaca ataccgatca gcaaaataat gccgataatc gcaatcacat ccagttcgct
     7081 accggcaatc attaatgcca gcagcgcgcc gacccctgcg gtgggtagcg tcgagagaat
     7141 ggtgatcggg tgaataaagc tctcgtacag aatgccgagc acgatataca tcgccaccac
     7201 cgccgcgaca atcagccaga cagtgctgcc cagcgccgac tggaaggcga gggtgctgcc
     7261 ctggaattgc gtggtgatat ccaccggcag attcagcgtc ttttcggtgt ccataatctc
     7321 ctgcaccgca tcgcccagcg aatagttatc cggcacgtta aaggagatgg tcgttaccgg
     7381 gaactgatcc agatggttga tggaaagcgg cgcaaaacgc tgttcaactt tggcaattga
     7441 gcttaacggc accacgccgc cgtcgctact ggtcaggcga atggtatcca gcgccgcgag
     7501 gcccggggta atttcggtgt tgtgctccag cactacgcga tactggttag cctgagtata
     7561 aatagtggaa atcagtcgct gaccaaacgc gttgtacagg gcgttatcga catccgccat
     7621 gctgatcccc agacggctgg cgctgtcgcg atcaacattg acatacgcca ccagcccttg
     7681 gtcctgccag tcgctggaga catcagaaag ctgtggcagt tgctggagtt tttccatcaa
     7741 ctgtggcacc caggtactga gcgcatccag tgaggtggcc tgcaaggtaa actggtactg
     7801 ggtgcggctg acctgagtat caatagtcag atcctgcgtt ggttgcagga agagatcgac
     7861 gcccggcact ttatctaccg ccgtttgcag acgggcgatg actttttgca cccgatcatc
     7921 acgttcatcc aacggtttga ggttgatttg taaacgtgca ctattcagcg acgggttagt
     7981 gccatcaacg ccaacaaatg aggtcaggct ttgcactgcc ggatcctgca aaatcacgtc
     8041 cgcgacctgg cgttgtcgct gggccatatt ggtaaaggag ctggattgcg gtgcctgcaa
     8101 agtgccctga ataatgccat tgtcctgtac cgggaagaaa cctttcggaa tgaacaccca
     8161 cagcagcacg ctaagcagca gcgtgctgag tgccacgctt aaggtcagcc acggatgatt
     8221 cagcactttc gccagtccac gaccataggc ggcgattatc ctgtcgaaca ttttttccga
     8281 ggcacgggag aagcggttct gtttacgcaa cgactcctgg ctgagcatcc gcgcgcacat
     8341 catcggtgtc agggtcagcg acaccaccgc tgagatcaaa atcgctaccg ccagggtaat
     8401 agcaaattcg cggaacagtc gcccgacgat atcgcccata aacagcagtg ggatcaacac
     8461 cgcaatcagt gagaaggtca gcgagataat ggtaaagccg atttcacctg cgcccttgag
     8521 cgccgccgcc aacggttttt cgcctttttc gatatagcgg gaaatgtttt cgatcaccac
     8581 gatggcgtca tcgaccacga atccggtggc gatagttaac gccatcagtg tcaggttatt
     8641 gattgaaaaa tcgagaaaca ccataaccgc gaaagtgccg attaacgaca gcggtacagc
     8701 aacaccggga atgatggtcg ccggaatatt gcgcaaaaac aggtagataa tcatgactac
     8761 cagcgcgata gccatcatca attcaaactg agtatcatcg acggatgcgc ggatattggt
     8821 ggtgcgatcg gaaagcactg tcaccttcac cgatttcggc agactctcag tgagctgtgg
     8881 cagcatctgc cgaatgctgt cggcggtgga gataatgtta gcaccgggct ggcgctgaac
     8941 attcatcaca atggcctgtt ctttgttcgc ccacgcgccg agccagctgt tttctgcacc
     9001 ttgctctaca gttgcgacat cgcccagacg aattggcgcg ccgttctggt aggcgatgat
     9061 tagctggcga tactcttcgg cggattgcat ctggtcgttc gcggaaagcg tgactgcacg
     9121 ggaagggccg tcgaggctac cttttgccga gttaacgtta gcgccggtaa tggcggtgcg
     9181 cacggtttcg ctggtcaggc cgagggcggc aatcgcctga gcgttaagtt tgacgcgaac
     9241 agccggacgc tgaccgccgg aaagcgtcac caggccgacg ccggaaatct gcgagatttt
     9301 ctgcgcgacg cgggtttcca ccatatcttc cacttgcgtc atcggcatgg cggttgaggt
     9361 gacggcgagc gtcatgatcg gcggatctgc cgggttcact ttgctgtaaa ccggcgggtt
     9421 aggcagatcg ctcggcaaca agttggtcgc agcgttaatc gcggcctgca cttcctgctc
     9481 ggcgacatcg agcggtaatg ttagctggaa ctgcaaagtg ataactgacg caccgccgga
     9541 actttgcgac gacatctgtt tcaggccaga catctgcccg aactggcgtt cgagcggcgc
     9601 ggtaacggca gaggtcatga catccgggct ggcacctggg tagagcgtga ccacctgaat
     9661 ggtcggatag tccacttccg gcagcgccga aacgggcagg gcgcgataac cgataatccc
     9721 ggcgagtaag atcgccacca tcagcagcgt ggtggcgaca ggacgcataa taaacaggcg
     9781 cgacgggccg cctgtgctgc tcgggggtaa cacctgcatc aggagcgtgc tccttttttc
     9841 gcgtattcgc ggctggtggc tttctcttcc ggagtggtgg cgctctgggc ttccaccact
     9901 tccactttcg ccccttcggt caggcgatca atgccgtctg tcaccacgcg atcgcccgca
     9961 gaaatacctg cacggatcac cactttctga ctgtcctgaa tgcccggcgt caccagatgt
    10021 ttgctgacct tgttttcgct attcagcacc cagacaaaat ggccttcatt gcccatttgc
    10081 agggcggctg ttgggatcac tacggcgttt tgttcggtgt cgactaacat gcgcgcgtta
    10141 acaaactgat tgggaaacag cgcatcatcc tgattattaa agcgtgcttt cactttaatc
    10201 gtaccggttg tggcatcgat ttggttatcg aggcttaaca gcgtgccttc acttaatttc
    10261 ttcgaattgg tgcgatccca ggcttctacc accagcggtt ttccggcttt ttgcgcctgc
    10321 actacagtag cgatatcgct ttccggcagg gtaaagacta aatcgatagg atgcgtctgg
    10381 gtgatcacca caattccggt ggtatcacca ctggagattt ggttaccaac atcaacctgc
    10441 ttgagaccaa cgcgaccatc gactggtgcg gtgatgcggc tccagtcgag ttgcaactgc
    10501 gcgctggcga cgcttgcttc atcagcctta atggtgcctt cggtttcact gaccagcgcc
    10561 tgttgggcat ccagctcctg acgggagacg agattggttt ttgccagttg ctgataacgc
    10621 gccagatcac ggcgggcgtt agcaagcgtg gctttatctt ttgccagttg gccctgggtt
    10681 tgtgctaatg caactttgaa ctggctgggg tcaatttctg ccagtaaatc gcctgctttg
    10741 acctgctggc cttcctggaa atgtaacgcc atcagttgac cgtccacgcg gctgcgcacc
    10801 gtaacagtat tagcggcgat aatggtgcca agcccggtga ggtaacgcgg aactgcctgt
    10861 tctacggcgg tcgccgcctg aaccggggct aatgggccgg aacgcatacc acggcgacca
    10921 cccgctggcg attgctgcgc ttgtttcgtc gcccctgggg ctgcactctg ggagtcattg
    10981 cggccttgcc agaaccagaa tgcggcgatg gcggcgataa ccaccacgat tacccaacgg
    11041 gatttataac tgcctttcat cgttaagagt ttctcttcct gaaacgtttc gcggaatgat
    11101 actagtgtag ccagccaata agggagaaaa atggaggaat tatgaaacac tgtcgggaat
    11161 cgtctgaatg acgggcacat ttgcgagcac gcatccagta ataacacagg aaactatttt
    11221 atctacgcgt tagcgataga ctgcttgcat ggcgaaagga ggtaagccgc cgatttcagc
    11281 gggacgctga aacgggaaag cccctcccga ggaaggggcc ataaataagg aaagggtcat
    11341 gatgaagcta ctcatcatcg tggtgctctt agtcattagc tggccagctt actaaggcta
    11401 ccagggcggg ggaaaccccg ctctaccctc actcctgaaa gtatgccttc acgataagat
    11461 tgtcaatctg caggctttgc agtctgcgat cctgccagca aatattcttt gcgagacgtt
    11521 acgcaataat cacactggaa actattttat ccgcaagtca gcgatagact gcatacaggg
    11581 cgaaaggagg taagccgacg atttcagcgg gacgctgaaa cgggaaagcc cctcccgagg
    11641 aaggggcctt aataaggaaa gggtcatgat gaagctactc atcatcgtgg tgctcttagt
    11701 cataagcttc cccgcttact aagactacca gggcggggga aaccccgctc taccctcact
    11761 cctgaaatta tgccttcacg ataagattgt caatccgcag gctttgtagt ctgcgatcct
    11821 gccagcaaat attctttgcg agtcgttacg caataatcac agatgaaact attttgttca
    11881 cgcgttagcg atagactgca ttcagggcga aaggaggtaa gccgacgatt tcagcgggac
    11941 gctgaaacgg gaaaccctct cccggagaag agggctttta ataaggaaag ggttatgatg
    12001 aagcacgtca tcatactggt gatactctta gtgattagct tccaggctta ctaagaacac
    12061 cagggggagg gggaaacctc tccctaaccc tcactcctga aattatgtca ctccgccctt
    12121 agtgtcaatc gacggctctc cgttcttgcg aggcgaatag caaaattttc tgcgccgacg
    12181 ggcctaattc tgaaagtttc ttcgtttgct cttatttcat tattgcaatc ggtatgatac
    12241 cggacctcga taattctcta agaaaggatt tcttatgagc gaacaaatca cgtttgccac
    12301 cagcgatttt gccagtaacc cggaaccacg ttgcccctgc attttgctgc tggatgtttc
    12361 tggctcgatg agcggcagac ctattaatga acttaacgcc ggattggtta cctttcgtga
    12421 tgaactgctt gctgatccgc tggctttaaa aagagtggaa ctcggcattg tgacgttcgg
    12481 cccggtgcat gtggaacaac cctttaccag cgccgctaac tttttcccgc ccatcctgtt
    12541 tgcccagggg gatacgccta tgggggccgc cattaccaaa gccctggata tggttgaaga
    12601 gaggaaacgt gagtaccgcg caaatggtat ttcgtattat cgcccgtgga ttttcctgat
    12661 caccgatggc gctccaaccg atgagtggca ggctgccgcc aacaaagtgt ttcaggggga
    12721 agaagataag aagtttgcct tcttttccat cggtgttcag ggggcggata tgaagactct
    12781 ggcacaaatc agcgttcgtc agcctttgcc actgcaggga ttacaattcc gtgaactgtt
    12841 tagctggtta tccagttcgc tgcgttcggt ttcccgctcc acgccgggaa cggaagttgt
    12901 gctggaagcg ccaaaaggct ggacgtcagt gtgagctggc gtctggttta tgcatccgct
    12961 gttggaacat cgcacattag tgctgatttg ccttgtcagg atgcctgcca gatgcaggtt
    13021 gtctggttaa acgaccagca accattactg gtgatgttcc ttgctgatgg cgcaggtagc
    13081 gtttcgcagg gaggagaagg cgcgacgctc gccgtcaatg aagcgatggc gtttatggtg
    13141 caaaaagtac aggacggtga attggggctt aatgatgtcc tcgccacaaa tatggtactg
    13201 actattcgtc aacgactttt tgccgaggca gaagccaaag cattagcagt tcgcgatttt
    13261 gcctgcacgt ttctgggatt gatttcatcg cctgacggca ccttgattat gcagattggc
    13321 gatggcggcg tggtggtaga tttgggtcac ggtctgcaat tgcctctcac gccgatggtg
    13381 ggtgaatatg ccaacatgac gcacttcatt accgacgaag atgccgtttc cagactggaa
    13441 actttcacca gcactgggcg tgcgcataaa gttgcagcat ttacggatgg tattcagcgg
    13501 ctggcgttaa atatgctgga taattctcct c
//
LOCUS       DAZPLI010000109        13335 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZPLI010000109 DAZPLI010000000
VERSION     DAZPLI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13335
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.110"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..312)
                     /locus_tag="ACHUU3_002995"
     CDS             complement(<1..312)
                     /locus_tag="ACHUU3_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFR2083006.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHUU3_002996"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHUU3_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR2083007.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHUU3_002997"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHUU3_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR2083008.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="ACHUU3_002998"
     CDS             complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="ACHUU3_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR2083009.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="ACHUU3_002999"
     CDS             complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="ACHUU3_002999"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR2083010.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="ACHUU3_003000"
     CDS             complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="ACHUU3_003000"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR2083011.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="ACHUU3_003001"
     CDS             complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="ACHUU3_003001"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR2083012.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="ACHUU3_003002"
     CDS             complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="ACHUU3_003002"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR2083013.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8292..9365)
                     /gene="lpfD-O113"
                     /locus_tag="ACHUU3_003003"
     CDS             complement(8292..9365)
                     /gene="lpfD-O113"
                     /locus_tag="ACHUU3_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR2083014.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(9376..11898)
                     /gene="lpfC-O113"
                     /locus_tag="ACHUU3_003004"
     CDS             complement(9376..11898)
                     /gene="lpfC-O113"
                     /locus_tag="ACHUU3_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR2083015.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSCVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(11923..12654)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUU3_003005"
     CDS             complement(11923..12654)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUU3_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFR2083016.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(12702..13274)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUU3_003006"
     CDS             complement(12702..13274)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUU3_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR2083017.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSLVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT         3505 a         3399 c         3006 g         3425 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccgaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc ccaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atggttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa
     3361 atatgaccat gctcgcagtt attaactttg tgtaatttta ggaatttata aagttatata
     3421 taacaaatcc caataattaa gttattggga tttgtctggt gaattatttg tcgctatctt
     3481 ttcccgccag cagtttatcc agctcatcac cgcctacatg acggaaatcc tgccccttca
     3541 cgtagtagaa gataaactca caaatattct ggcagcggtc gccaatacgt tcgatagaac
     3601 gcgcgcagaa cagcgcagta agtacgctcg gaatggtacg cgaatcttcc atcatgtagg
     3661 tcatcagttg acgaacaata ccttcgtatt cctgatcgac ttttttatct tcacgataaa
     3721 tacgtaccgc ttcgtcaatg tccatccgcg cgaacgcgtc cagcacgtcg tgcagcatct
     3781 ggatggtatg acggcccagc gactccagac ttaccagcaa cggctgatgc tgctgggaga
     3841 atttctccag cgcagtacgg cagattttgt ccgccacgtc gccaatacgc tccagctcgg
     3901 caatggtttt actgatcacc ataaccagtc gcaggtcgct cgccgtcggc tgacgtttgg
     3961 cgataatgcg cacgcaggct tcatcgatcg ccacttccat catgttgacg ttcttgtcgc
     4021 cttcgatcac gcgcttcgcc agatcgctgt cctggttatg catcgcggtg attgcatcag
     4081 aaagctgctg ctccaccatg ccgcccatgg tcatcacctg cgtgcggata ctttccagtt
     4141 cggcgttgaa ctggccggaa atatgtttat taagattgag actgtccata acgcactcct
     4201 gaatcaaccg taacgaccgg tgatgtagtc ttctgtttgt ttcttcgctg gcttggtgaa
     4261 cagatcgtcc gtgttgctga actcaatcaa ttcgcccagg tacataaacg ccgtgtggtc
     4321 ggaacaacgc gcagcctgct gcatgttgtg ggtaacgatc accacggtgt aatcctgctt
     4381 cagttcggtg atcagctctt caatacgccc ggtagagatg gggtcgagcg ccgaacacgg
     4441 ttcgtcgagc agcagcactt ccgggcgaat ggcgatacca cgcgcaatac acagacgctg
     4501 ttgctgacca ccagagagag agtaaccgct ctggtgcaat ttatctttgg tttcgttcca
     4561 caatgcggct ttggtcaatg cccactgcac gcgctcgtcc atgtcggcac gggagagctt
     4621 ctcaaacaga cgaacaccaa aagcgatgtt gtcgtagatg gacatcggga acggcgtcgg
     4681 tttctggaac accatgccca ctttcgcacg cagcagtgcg atatcctgag agttggtcag
     4741 gatgttgtcg ccatcaagca gaatttcacc ttctgcgcgc tgctccggat acagttcaaa
     4801 cattttgttg aaggtacgca gcagcgtcga tttaccgcag ccggacggcc cgataaacgc
     4861 cgttacctgg tttttagcga tatccaggtt gatgtttttc agggcatgga atttgccgta
     4921 gtagaagttc aaattacgaa cctgaatttt actcggggca gtttcaacca tactcattgc
     4981 aatcttttcc tcatcgtacc tgatgctttt cgctaatcag gcttaccgta ggccgggtaa
     5041 ggcgttcacg ccgcatccgg caaagtgtta accgtgttta ttcttcgcaa aaacaacgcg
     5101 cgccagaatg ttcagcagca ggacgcacag ggtaatgatc aataccccgg cccaggccag
     5161 ttgttgccat tccgcaaacg ggctcatcgc aaacttaaag atcgtcaccg gcaggttggc
     5221 gatcggctgc atcatgtccg tgctccagaa ctggttggag agcgcggtaa acagcagcgg
     5281 cgcggtttca ccggcaatac gggcaatcgc cagcaggata ccggtcataa tcccggacac
     5341 cgacgctttc agcgtaatcg cggagatcat cttccacttc ggtgtaccca gcgcataagc
     5401 cgcttcacgc aggctgtccg gcaccagttt cagcatgttc tcggtggtgc ggataacgat
     5461 cggcacctgc aacagcgcca gcgcaattac gcccgcccag ccggagaagt gctccatctg
     5521 tgccaccacg atggtgtaga caaacagacc aaccacaatc gacggcgcgg agagcagaat
     5581 gtcgttaatg aagcgaatca cttctgccag ccaggattta cgaccatatt ccgccagata
     5641 aatccccgcc ataatgccca gcggcgtacc gaataccgtg gcccacaaaa ttaacagccc
     5701 gctacccgcc agggcgttcg ccagaccacc accttccgta ttgggcggcg gcgtcatttc
     5761 agtgaacagc gccagcgaca taccgtcgat accgcgagtg atggtggaca ttaaaatcca
     5821 gatcagccag aacaggccga aggccatcgt cgccatcgag agcgtcagcg caatacggtt
     5881 tttgaggcgg cgacgcgcct gcattttgcg gcgagattca gccagcgccg cagtggtttg
     5941 catttcaacc atagccatta gcgtgccccc tcattcttag ccaggcgcat aatcataaac
     6001 ttcgatgcgg cgaggacgat gaaggtaatc acaaacagga tcaggcccag ttccatcagt
     6061 gcggcaacgt gcagaccgga ttccgcttcc gcaaattcgt tcgccaacgc agaggtgata
     6121 ctattgcccg gcatgtacag cgaggcgctg tcgagctggt aggtgttacc gatgataaag
     6181 gtcaccgcca tggtttcacc gagcgcgcgg cccagcccca gcatgatgcc gccgataaca
     6241 ccatttttgg tgaacggaag aacgatacgc cagataactt cccaggtggt gcagccaata
     6301 ccgtaggccg actctttcat catcaccggg gtttgttcga acacatcacg cattaccgcc
     6361 gcaatgtacg gaataatcat gatggcgagg atcacgcctg ccgcgaggat accgatacca
     6421 aatgcggggc cagagaacag cgcgccaaca atcgggatat tcgacatgat attgccgacc
     6481 ggctcctgaa agtaaacggc gaacagcggc gcaaagataa acaggcccca catgccgtaa
     6541 acgatacttg gaatggctgc cagcagctca atggcgatac ccagcgggcg tttcagccag
     6601 ccaggcgcaa gctcagtcag gaacagggcg ataccgaaac tcaccgggac ggcgatcagc
     6661 agcgcgataa acgaagtcac caacgtaccg tagatcggca ccagcgcccc gtagatatcg
     6721 ttcggtgcat cccactcttt ggtccatagg aaagccagac caaatttctg aatgctcggc
     6781 caggaggaga tgatcagaga gacaataatg ccacccaaca tcaatagcac aatcagcgcc
     6841 gccagtttta ccagcacgct gaaaattatg tcgccctttt tacccggtgg gttaaaagca
     6901 ggcttggttg cagccataag ttactcttca gttaaacgcg tttacaaagt cattttgtaa
     6961 tgccggatgc ggcgtaaaac accgtacccg gcctagagtt ttattagtac agcggcttac
     7021 cgctactgtc tttaatattg gtcttccacg cagcgcgaac ctgttcaact acactatccg
     7081 gcaggctggc gtaatccagg tcgttcgcct gtttagcccc ggttttgtac gcccagtcga
     7141 agaatttcag cacttctgtg ccttgttctg gtttcttctg atctttgtgg atcagaatga
     7201 acgtggtaga ggtaataggc catgcatctt cgcctttctg gttggtcaga tcctgagcga
     7261 aggttttgct ccagtctgca ccttttgctg cattagcgaa gttttcttcg gtcggactaa
     7321 ccggtttacc atcagcggag atcagtttgg tgtacgccag gttgttctgc ttcgcgtaag
     7381 catattcaac ataaccaatt gcacccggca gacgctgaac gaacgcggcg ataccgtcgt
     7441 tacctttacc gcccagaccg atcggccatt ttacggtaga gccagtacca acgttgtttt
     7501 tccactcttc gttcactttc gccaggtagc tggtgaagac gaaggaagtc ccggagccat
     7561 ctgcgcggcg tactacagca atgttttgtg aaggcagttt cagacccgga ttcagtttgg
     7621 cgatggtttc atcatcccac ttcttgattt tgcccaggta gatgtcgccg agggttttac
     7681 catccagcac cagttcgcca gacttcagcc ctggaatgtt aaccgccagc accacgccgc
     7741 caatcacggt cgggaactgg aacagacctt cctgagccag tttttcgtca gacagcggcg
     7801 cgtcagaggc accaaaatca acggtattag cgataatctg ttttacgcca ccggaagaac
     7861 cgataccctg gtagttaact ttattaccga tttctttctg gtaagtgtca gcccatttgg
     7921 catacaccgg cgcagggaag gttgcacctg cacctgtcag gcttgcttct gcaaacacag
     7981 agaaagcgct catcgataag gtcgcggcga caacagttgc gacggtggta cgcataactt
     8041 tcataatgtc tcctgggagg attcataaag cattgtttgt tggctacgag aagcaaaata
     8101 ggacaaacag gtgacagtta tatgtaagga atatgacatt tttatgacag aaaggaatta
     8161 acaaataact tactgatttt aaatgtgaag atgaaaatcc cacatgtcat ctttttgtca
     8221 tgatgcattt aaaagtgata aagaaatggg tgagatgaca acgatgtgat gaacaatcgt
     8281 tgtcagacct attagcgcac aatctcaaca gttatcgtcg ctgttgcagt aaattgcccc
     8341 ggggctgggc gagctccagt aaggctcgca ggagcagctg aaaaagttac actacctgat
     8401 ttttcaccat acactgttga tttacccatg tctgttggca actcaccacc gttcactttg
     8461 atttccttat tatttttgtc gtaaattttt atccccacgt tagagtttga agttacgacc
     8521 attgaattgt ccgtattggg ttcggtagca taaaaagacg tactgatatc atcggtattc
     8581 agtatcccca ggttttcaca ctcaatagtg actgttttag taattttatg tgttgttacc
     8641 gcacttccaa caacagatga aaaatctgca gctggtattt ctttaaaatc aaaataaact
     8701 ggctgtaaat tatttatttc gcaactttgc ggagctgtaa tatcgccccg aatataaaca
     8761 tcagccatcg gctgtgagta atccaccggg gtgtcacggc taattgttcc atacaatgtc
     8821 actatctttg tttcaggaat aatcaatttt cctgcaaatg tcttcttgat ataaaaatat
     8881 actatcgctt tacccccact ggatatactt aaaggttcaa tgcggttgca attgtacttt
     8941 gtattaccag acgggttgtt agcatgatat tcaaaaggtg cattcacata cccaacccca
     9001 agaatatcta tagcaatccc aacatcaagg ttcggattaa gatcataata attaatatta
     9061 gagtgaaccc cagcataagt taatgcctgg tttatttttg acgtataata gatatagggg
     9121 aattctccta cattaccagt atcacaatga cagcgtccag gatatgagga tccattagtt
     9181 acagtggtat taaatgtcgt tccaacctta ttttcgttcg cggtaataag atatcccgag
     9241 aaatcaagcg tacggtgaaa tacgccatta tcaggggtac agataccttt aggagctgca
     9301 agtgctacac tacttaacga gagcataaac acagcaaagc accactgttt gatatacttg
     9361 ttcataaaaa tccttttaat gacatgtcac accctgttca attaatccgc tctcactttc
     9421 tggtgtcgaa agtgaataat tgaagatgca ttttgttgag gcgttttctc cccaggataa
     9481 cgtaacctgt cctttttctg gtagaccaga aagataaagt tcgccacggt caccgacaat
     9541 tcctgaatct gccaaacttg catcttgtat cgcagcaatg gctccaaaag gtaccggttt
     9601 accatttgca tttaacactc tgaagagaac gcgataaccc acatgagtca caaactccgc
     9661 tttaaccact gcaccacgcg tgggaaccat cgtaacgacg gtattatcaa gttctatatt
     9721 cgacttcgag aaatagttat cgctgattgc tacccgattt tcatcataag ggtttagctg
     9781 tgtcttgacc gtatagccgc gccagtccgt cgcagcaccg cgcatattat ctatttctaa
     9841 cccggcggcc ccaggtgctt taacaagagc aatagtttca cctaactcct gacttaacgt
     9901 taccccttcg ctgtgtgcca gaattccccc tctgatacca taattcagcc tctgggaatt
     9961 ttggctgtag ttatagctgc cgttcaccgt gccataagag ccatcatagg tcagggacat
    10021 attaccgcca acctcatcat catgactgtt ataggactgc attaaattcc atgaaaggtt
    10081 atttctttcc agcgccgtac cgctgagtcc gagttgatgt gagctaccgg aattatcgtt
    10141 ttgcgaaaca gaataggtgg cgtaactatt accccaccag cccaataaag gaacagataa
    10201 agagagcatg tagatctgct cactggtgtt atcactggca tggttgttcc ccgtcccctg
    10261 tctggcagta ttggaataac tccaggagac gttccacgag agacgcttcc agctactgct
    10321 gtagccaaat tgcattaatc gttcgacatc atcggtatgc cagtattgtt gccggcttaa
    10381 tgtcaggtat aaattgccat aatctctccc caacgactgc gttagcgtcc cctccacgca
    10441 actacgtcgg ttacctgttt cccaaaagtc ttcagggctg ttgcgccgcg atgcccactc
    10501 attgagggta tagtagccct cggtggagta acgatagcca accaagcgca agctagtgtc
    10561 tgtgtcgtca aatcgttttg aatagagaaa gcgatatgac tgacccgttt ctgtatcatc
    10621 atggctaaaa ttcgcacgag catgtgtcac atcgaaagaa attgcgccca aagcgccaag
    10681 atctttaccg acacccagag ctgcagaacc ataattttca gcagcctgca tacctccata
    10741 taaagtgatc ccgtgggaaa aaccatgaag tatttgcgcc tgaaggacgt ccggtgtaaa
    10801 tccgtcttca tcgcgcaatt cacccacgct gacatcaaca tctgtctgac cttcacgttt
    10861 aagaatcgct aatgaagcat aaggttgggt gaatcgttgt tcgctaccgt cttcttcttt
    10921 gatggttacg tccaaatcgc cacctgtact ggcggtgttg agatcggtga tctcaaaagc
    10981 accttgtgga acggcagact gataaatcac gtagccattc tgccgaatga tgacggtggc
    11041 gttggacttt gcaatcccac gaaccacagg agcaaagcca ttctggctgg cgggtagcat
    11101 atcgttatca gtatacaaac gcgcgccgcg aatttgcgta ctgtcgaaaa tatcgctcgc
    11161 cgtccacgta tcaccaatca taatctggct tcttaacgcg gcaatatcgc gctgtatcca
    11221 ggtactaatg gagttgtatt ccgcacggcc atcgctggtg tttaaagtac tgtaattacg
    11281 tagccgccac ggtccaatat tcagtccatt gcgcagattg agataactgg tatctgtctc
    11341 ttgtgaacca taatctgaac gtagtgtatg tgaaccagaa aaatcgtagt tgatggtgag
    11401 cgcgttaatc ccctcatccc aacgcgtggg gctgatgtaa tcataagcca tcgcgtccat
    11461 gtagatttgg ggaatggata tattcagctg caatttatca ctgtcaaact gccaggatga
    11521 gccttcaata cgtggagcag gatcgaggca aaccgtctca tccacatcat tgaacgattt
    11581 gagcgcatca acttttatgc caaaggagga cagtgttttt ttaatggcaa taaagcaagc
    11641 gtgcagatca gcatctggat tatcctgaac aaatgtcaca tcctgacgtt caaacatttt
    11701 accgttgata aaaatatcaa cgctataaac acctgggggc atcaggtttg cccgattaaa
    11761 ctggcgaata tcgactccgg attgatggtc gatttccaga agcgccggat tgaatacact
    11821 gacctcggcc atcaggtttt gcgagaaaat cagacacgtc cctgccgtga ggccaaccac
    11881 tattctggtc gtcatcataa tacgcctatc cttatccctt gcttacagcg tcatttttat
    11941 cgggtcactg actgcgccgt aatcattaat aaatcgccag gtcatttggc ctcccgcaga
    12001 attagcaggt aaaggtaacg tgagcgtttc aaaaggacgg atcatattga ggggcacatc
    12061 atggccatca acgatgacat ccatcacact gatatgaatt gggttgggat tctctgctat
    12121 cagatttcgc ccttcccggc gccatttcac tttcggcgca gcatcttcgg gaataaggcg
    12181 gatatttgct ggtcgccaga acagcttgat acgcgttttt actgctattt ccagcgagtt
    12241 cgtcgctttg ttgctcgctg atggcggaat atttttaata ttcagccaga agactgactc
    12301 gcgatcatca ggcaatgaca cacctttggt gtgaataatg cgtaatattt tttcttgcga
    12361 aggttctaat cgtgatactg gcggaataac ggtaaacggc gctttgcttt tatcctcttt
    12421 attaaatgga tctacccaag cctggatgag ataaggtatt ttttcttctt tgctaaatac
    12481 agaaatagat ttatcagcat tattacttaa gtaaaccacc cgcgtaccac caatgacaac
    12541 gcctgcataa gttaatgctg gttggcctaa aaaaacgctg agtataatcg cggtaaataa
    12601 cttacatgaa actgttgttt taaagagcct gattaaacaa ctttttctgg acataacgac
    12661 tccctggcca aagccaactg aataaaaagg cccttgataa attatttctt atattcgaca
    12721 gtaaattgtg aatcagcgtt cgccgtacca gccgttacgg tcgcattggt tgccacatag
    12781 cgtgcaataa acttcatttc agccttgcct tcagcatcga tagcggtata agcagagtcg
    12841 ttataaagtt taaccagcga attatcggac tggttaaaaa tacgaatacc aacgccggtt
    12901 gccgcaatcg cttgtcctgt accggtaaaa tcaccagggt tgcctgctgt aagcggcgta
    12961 cccactttca ggtcgccgtc gccgccaggc gcttctgtac catcaaaacg aacggctgct
    13021 tgagtgtacg ttgccgggca attttcaagt ttaatactga acttctgtgg cgacgccaga
    13081 ctacctacac ctgaaaatgc atttttactg actttaccca gtgggacaat ttgatcggct
    13141 gtatcggagg taacttcaca agtagtgtcg acaatttcac cgacgaagtg aacaacacca
    13201 tcttctgcca gtgcatgccc ggccagcatt agagatgcca gagtgaatgc accgcctata
    13261 atattacgct tcataaaaac tccattgata taatatatcc tttaccatga atcatcactt
    13321 atattaataa gtgta
//
LOCUS       DAZPLI010000110        13262 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZPLI010000110 DAZPLI010000000
VERSION     DAZPLI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.111"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..843)
                     /gene="adrA"
                     /locus_tag="ACHUU3_003007"
                     /gene_synonym="dgcC"
     CDS             complement(<1..843)
                     /gene="adrA"
                     /locus_tag="ACHUU3_003007"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR2083018.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(945..1265)
                     /gene="psiF"
                     /locus_tag="ACHUU3_003008"
     CDS             complement(945..1265)
                     /gene="psiF"
                     /locus_tag="ACHUU3_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR2083019.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1384..2799)
                     /gene="phoA"
                     /locus_tag="ACHUU3_003009"
     CDS             complement(1384..2799)
                     /gene="phoA"
                     /locus_tag="ACHUU3_003009"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR2083020.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2900..3160)
                     /gene="iraP"
                     /locus_tag="ACHUU3_003010"
     CDS             complement(2900..3160)
                     /gene="iraP"
                     /locus_tag="ACHUU3_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR2083021.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(3342..3545)
                     /locus_tag="ACHUU3_003011"
     CDS             complement(3342..3545)
                     /locus_tag="ACHUU3_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083022.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            3623..4717
                     /gene="ddlA"
                     /locus_tag="ACHUU3_003012"
     CDS             3623..4717
                     /gene="ddlA"
                     /locus_tag="ACHUU3_003012"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR2083023.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(4741..4953)
                     /locus_tag="ACHUU3_003013"
     CDS             complement(4741..4953)
                     /locus_tag="ACHUU3_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR2083024.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            5213..5521
                     /locus_tag="ACHUU3_003014"
     CDS             5213..5521
                     /locus_tag="ACHUU3_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR2083025.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(5580..6674)
                     /gene="yaiW"
                     /locus_tag="ACHUU3_003015"
     CDS             complement(5580..6674)
                     /gene="yaiW"
                     /locus_tag="ACHUU3_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFR2083026.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(6687..7907)
                     /gene="sbmA"
                     /locus_tag="ACHUU3_003016"
     CDS             complement(6687..7907)
                     /gene="sbmA"
                     /locus_tag="ACHUU3_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR2083027.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            8259..9416
                     /gene="ampH"
                     /locus_tag="ACHUU3_003017"
     CDS             8259..9416
                     /gene="ampH"
                     /locus_tag="ACHUU3_003017"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR2083028.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(9417..10040)
                     /gene="iprA"
                     /locus_tag="ACHUU3_003018"
     CDS             complement(9417..10040)
                     /gene="iprA"
                     /locus_tag="ACHUU3_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFR2083029.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(10128..13016)
                     /locus_tag="ACHUU3_003019"
     CDS             complement(10128..13016)
                     /locus_tag="ACHUU3_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR2083030.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         3370 a         3314 c         3153 g         3425 t
ORIGIN      
        1 accgcgcagg gtaatttgta actgtcgggt aagcgccaca atcgcttcat cgcccacatc
       61 atgaccccag gtatcgttga tgctcttgaa atggtcgata tcgataatca gtaacgttgc
      121 atcgcgatta tgccgccgac agttatcaaa ttcattgcgt aacatagttt cccaatgacg
      181 tcggttatac acgcccgtca tgccgtcgcg agtactcatg acctgcaacc tgcgtttatg
      241 ttccgccagt ttggttgccg tctggtagct gacccagcca aacagcagag gataaacgac
      301 aataatggga agggagagcc accattccag cggcgcacta ttgaacgaca cggtaatgcc
      361 cgtaagctcg agggtgacaa ggcaggaaac caccatcaac accagacccg cgacaaacag
      421 acgggggccg cctgccccca tcaaattcag acacataatc atcaacatag cggtggaagg
      481 cagcacgttt acgcccatta cgcctaccca cattcccgct aatactgcat cggtttttaa
      541 gttgtaaatt tcccggctaa gcggatcgac ggccctgctc gctatctgcc aggctaaatg
      601 cggccagacg aacgcccagc cgaccaacac cagccaccac cagcccggcg gcgggtgcga
      661 aaccagcgtt gaagcaatcg gtaagaacat gccagccagg ccaaccgcac ggggtagtct
      721 gacgcgacgg gcgaagcgca gcccggaccg ctgatgttcg ttttgaggag ttaaaggagg
      781 ttcctcccca tgcgccgccg cttttttgaa aaagttttca tcattcatta tttttgggaa
      841 cattcagaaa caattttccc aaattataga gacggggcta aaggcagaag tatgaaattt
      901 cgggtgagcc actttcgtag ctcacccgtt agcactatca ggtatcaggc ggctttcttg
      961 aggcaggcac tcataaactt attacgatca tcacctttca gagattgttg cgttgcttga
     1021 ttattacatt cgcgcatctt ttgctgctgt ggcgtcaaac ttttttcacc aggcgcagac
     1081 ttgctgttct tcaggcaatc actcatgtag gtcttacgag catccccttt caacgcctgc
     1141 gccgtcgcct gctgattaca ggaggtcata cgctgttgtt gtggggttaa ggttctctcg
     1201 gcagcgccga cggtggttaa aaaaaccaga ccaaaaagca aggtaaccag taatgttatt
     1261 ttcatagcac catccctctt catgttttaa ccatgagcgt ttgcgcccgt gatctgccat
     1321 taagtctggt tgctaacagc aaaaaaacca cccggcagcg aaaattcact gccgggcgcg
     1381 gttttatttc agccccagag cggctttcat ggtgtagaag agatcggtct ggtcggtcag
     1441 tccaacaaca ttggcggcat gcggaccata cgccgcaata cgcaactgac tgccggtatg
     1501 ttcttgtgaa tcctcttcgg aattcccgta actcatcacc atcactgcgc catctttagt
     1561 atttagcgcc tgagtgaggc ccggcgcttt ggtatccggc gcaacaacct ggctggcgtg
     1621 ggcgtgatca gcggtgacta tgaccagcgt gttaccctcc tttttagcga attccagcgc
     1681 ccgttgtacg gcttcatcga gatcgaccgt ctcgccaatt tgcccacaag gattcgcagc
     1741 gtgatcctgt ttatcgattg acgcaccttc aacttgcagg aaaaatcctt tctcattttt
     1801 actcaacaat tcaatggttt tgtcggtcat ctgtgccagg gttggtacac tgtcattacg
     1861 ttgcggatta ggcgtacagg tgactgcggg cttatcgata ttgccgtggt acgttgcttt
     1921 cggccctagc cagcgcactg gcatattgcc gtcagcaaac aggccaagca ggggtttttg
     1981 ctgattcgct tccgtcaccg aattcagtga ggcagcatcg ttcaccaact gataaccacg
     2041 cgcctgtgcc tgttcacgca gcgtttttcc ctgccattca ccagcggttg ccgtttcagc
     2101 aaaggttttt gcgccgccgc caagcgtaac gtcggcacga gcgttaagca gctgttcggt
     2161 aatcgatcct tttccgcctt tttccagagc gttacccgga catttttcac tggtcgcgct
     2221 cggaccgtag catttgcgcg aagtcacatg tgccaccagc gcagcgggcg tggcatcctg
     2281 caactctgcg gtagaaacgt taccggtcgc cagacctgcg gcttttgcca tttccagaat
     2341 cgttgggtga tctttttcgt gaatatcgac gcccagcgcg ccgttatagg ttttgacacc
     2401 ggttgaccag gcggttgctg atgcagccga gtcggtgacg tagtccggtt tgccagtttt
     2461 tttattcagc gcatagtgag tgtattgccc ggtaagcggt aaggcatcga tacctttaaa
     2521 aaagccgccc gcaccttcgg cataattacg tgcggcagta atttccgaat cccccatccc
     2581 atcgccaatc agcaaaataa tattttttgc aggtttatcg ctaagagaat cacgcagagc
     2641 ggcggtctga tcactcgtta agcggcgagc accgccgggt gcagtaatat cgccctgagc
     2701 agcccggttt tccagaacag gcatttctgg tgtccgggct tttgtcacag gggtaaacag
     2761 taacggtaag agtgccagtg caatagtgct ttgtttcact ttattttctc catgtacaaa
     2821 tacattaaaa aataaaaaca aagcgactat aagtctcggc cgtgacaact ttatgacagc
     2881 tgttgaaaag attaactttt tactgacgag gatgcttcaa taacttcttt acgtaatcgc
     2941 gcagcagctc cgtatcgtcg tcaggaatgc tggcatcggg ctttacctcg tacagcgccc
     3001 cctctacctg atcaatcaac cgctgttggt cattttgcgc catattgcga agcattgcag
     3061 tgacgataat ctccaaagct tctacctgcg cacacagttc tttcgactct tcttcttttt
     3121 gggcaagctt aaataacaac tcagcaatga gatttttcat gtctgtattt ccttatccaa
     3181 agtatggaga agttaacatt atgtttcaca acttcatagt gtctggaagt attattttta
     3241 tgacaaagga aatactttgc gcaaatattt tttgtttgat taccagcgaa acgtttctct
     3301 tatttaaata attacccaca caaaatatag gcttttaatt atcaaccttt attataaata
     3361 aaagttatga aatatattct cttcgttaac ttataattaa gagaaaaaaa ctcaccgcat
     3421 tgccatttaa gccaggaata aattagcttc ccgttcatca tttttgtgat ctcgcgcaca
     3481 tctttgagat aacgcacttt cttcaccagt acgttgcacg ctttgtcagc agaagattgc
     3541 aacaatcttt cagaaggccg cgaatccccc gttttgtcgt ggtaatgtat gcactttgtg
     3601 ttagacggga taggttttta agatggaaaa actgcgggta ggaatcgttt ttggtggtaa
     3661 atcagcggaa catgaagtgt ctctgcaatc ggcaaaaaac attgtcgatg ccattgataa
     3721 aagtcgcttc gacgttgtgc tgctgggcat tgataaacaa gggcaatggc acgtcagcga
     3781 tgccagcaat tatctgctaa atgcagacga tcctgcccat attgcgttgc gcccttcggc
     3841 gaccagcctt gcgcaggtgc caggtaaaca tgagcatcag cttatcgacg cgcaaaacgg
     3901 tcagccgttg ccgacggtgg atgtcatttt cccgattgtc cacggtacgc tgggcgaaga
     3961 tggttccttg cagggaatgc tgcgggtcgc caatttaccg tttgtaggtt ctgatgttct
     4021 ggcttcagca gcctgtatgg ataaagatgt caccaaacgt ctgctacgcg atgccgggct
     4081 gaacattgcg ccatttatta ccctgacgcg cgctaatcgt cacaacatca gttttgccga
     4141 agtggagtct aaactggggt taccactgtt tgtaaaaccg gctaatcagg gctcttctgt
     4201 tggtgtcagc aaagtaacca gtgaagaaca gtacgcaatt gccgtcgatc tggcgttcga
     4261 gttcgatcat aaagtgatcg ttgagcaagg gatcaaaggt cgtgagatcg aatgcgcagt
     4321 tctgggcaac gacaatccgc aagccagcac ctgtggcgag atcgtactca ccagcgattt
     4381 ctatgcctac gacaccaagt acattgacga agatggcgcg aaagtggtag ttccggcagc
     4441 cattgcacca gagatcaacg ataagatccg ggcgattgcc gttcaggctt atcagacgtt
     4501 gggatgcgca ggcatggcgc gtgtagacgt gtttttaacc ccagagaacg aagtggtgat
     4561 caacgagatc aacacactgc ctggcttcac caacatcagt atgtatccga agctgtggca
     4621 agccagcggt ctgggttaca ccgatctgat cacacgtttg attgagctgg cgctggagcg
     4681 tcacgctgcg gataacgcat tgaaaaccac aatgtaatat tttaacgccc gataatattc
     4741 ttattgggcg ttttcagttt cttcatcgcg acgacgaata atcaaccccg ccagccagaa
     4801 actaatcacc cacgtcacca gaccgacggc ataggtctgc caaccttttg cttcaaatcc
     4861 cagtaacccc accacgccat taagaatgaa tataaggcca atggcgaacg catagtagtg
     4921 ccagtcacgg cggattttta caggcagatt catggcagct ccgttaacga aaaacaacat
     4981 ccttgcatat tctgtaacaa ccgtctgtgg tgtagacaac attttcgtaa ttgttaataa
     5041 atttaactta aggttgccat tttgtaactc acaacgaaga ttcacgcttc gggaatattc
     5101 ccaacatcaa tttaccatga ttctgatatt gacaatctaa atgacctgtc agacttgcca
     5161 taagccgctg gtatcacacc agaagagaag cagatgactt ccggaggttt ttatggctga
     5221 tttcaccctg tcaaaatcgc tgtttagcgg aaaatatcgc aatgcctctt caacgcctgg
     5281 caacattgcc tatgcgttgt ttgtgctgtt ttgcttttgg gctggggcgc aattgctgaa
     5341 cctgttagtg catgcgcccg gcgtctatga gcgtttaatg caggtccagg aaacaggtcg
     5401 cccacgggtg gaaattggtt taggtgtcgg caccattttc gggctgatcc cgtttttagt
     5461 aggctgcctc atttttgcag tggtggcgct atggctgcac tggcgacatc gccgccagta
     5521 agatttacgt tgccagatac ggcctaaaac gccttatctg gcatgacatt cctgtttgat
     5581 cactgtttca tacaacgcgc ccgccgttcg tctacgcgct tcgcaaacca ggccgtagtc
     5641 aggttgcggg tgattttcgg gctttccagt tgaatgccag gcaacatttc gcggggtagt
     5701 gatttacccg ttttcgcttc ggcaagttga taaactttct tgtacagcgc cgtctcctca
     5761 aaagagaagc tgtcaccttt ctctaactga cggcgaattt cgctgtcgct catccccagt
     5821 tttcccgcca gtttgcgcgt tgccagttcg gttttcccgg gttctttact gtcgtaacga
     5881 attaaatcac cgtccagtgc cagcttcacg ccgctggcct tactgactgc gttctgaaac
     5941 gcggcattac ggctggcgta ccagccagcg ttaaaatcag caaagcggta tatcggtgcg
     6001 ctatagctgg cgggataatt cagtaaatgg taagtaccaa accacaaccc gccgcggcga
     6061 ctgaagactt cctgacgaac tgtaccgtcc attttccacg gatacccttt ggtatgctgt
     6121 tcggcaaaag caatgctgac ctgcatcggc ccaccggtgc gcaccggatt aagcgaacca
     6181 aacagcgtct gccccattgg taccatgctg atcagatcgt caaaaatcgc gctcaattgc
     6241 ttttctgtac gcaccgaatc taaccgttcg ctataacttt tgccgtttgg cgatttgatt
     6301 tttagcgcgg tatgaaccag aaaagcagga atgtgcatcc gttcagcacg acggtcaatc
     6361 tcttgccagg cgatcttgct taagcccgga acggccggat ctgcctgata atttgactcc
     6421 tgctgcgcca ccgccagcac cgagcagacg ttttccagcg tcggtgccag cccctgactt
     6481 tcaaaggtga tggcgagatc tttcgcccag gcatcgcggt ctttcacgct cgcaggcatc
     6541 ttctgccgca cgacgctcgc tacatctatc gctttttcgc cctttttcaa cggttgcgga
     6601 gcctgactac tacaccccgc taaaaccaga accgccaaca atgacagcga agatagatga
     6661 ttgacgcgcg acattacttc tcctttttag ctcaaggtat gggttacttc ctgaattttg
     6721 tcaccatcca gctcatgttc aaagctgcgc agacgtttgt agatagacat caactcaacc
     6781 agtgtggtcc atgagttaat caggtactgg aaagcaccgc gaacctgacc aaaaacgttg
     6841 gtaatctgcg tcatcaggcc gagcgtaatc gtaccggcaa caattgacgg aaacagcaag
     6901 aacaaaccga aaacgttatc gacctgcaaa tagagaatgc gggcgatgtt gaaatacata
     6961 tagtgaaaat agaggcgaaa atagtttttc cgtacggcgc taaacagctc gcgtaccgta
     7021 ggcggcgtcg cgcgcgtggc atcgtcttca ccataaacca gctctttacg gtaggcggcc
     7081 tctactcgct ggtttttaaa ctccagcccc ggcagtttga tccctaccac tgccagccat
     7141 ccggtcccca tcagcgacca gacgatagcg gcaatcacca gaccatacgg aatgtgcccg
     7201 ataatcggca gctccggcac atgcgcggag agcgttacca gcaccggcag gaaggcgatc
     7261 aacgtcatga tggcgttgat aaaactgacg cccatattct ccagcgttga agcaaaacgc
     7321 atggtgtctt cctgcacacg ctgtgcggca ccttcgatat gacgcagttg ttgccagttc
     7381 gccatgtaat attcgttcat cgccgtacgc cagcggaaca catagtgact gacaaagaag
     7441 ttgttcagta cactgatcac caccgcgatc agcgcaatcc ccagaaagac gcccacttcg
     7501 cggtaaaatt gttcgatagt gactttatgc ggcgaactta gcgcggtttg aatcaaatca
     7561 tagaacggtg cataccaggc gttgacggcg accccgactt ccaccaaaaa ccaggtgacg
     7621 aagatgatca gtgcagtacc gagtattgac cagtattgcc aacggtgcgg gctgtagata
     7681 aaccagaaaa atgcaaaaag tcctacgcaa acaatgtagt aagcgtaaaa aatcaggaaa
     7741 tccaacgacc agaaacgcgc ggcgctaatc gggatctgcc cggaagcgcc ggtgatacgc
     7801 gccacccagt ctcccccacc ggcttgccag aagataacgg caatcaatgc ccaaacaaag
     7861 gccgagagaa aaaacgctcc cggctttggg aaaaaagact taaacatatg cactcctgct
     7921 aacttcttat cgttttaaca actgctgtgt attaaccgca tgaccactac aaagcgtcag
     7981 ccaaatgaaa ccgcccattg tgacccatag caaggcgctt agttcgccag cctgaccata
     8041 agaattgttt cgacggattt cacaaaccaa agattgttta cataagcgga ttaacacgcg
     8101 caggatcaca aatgcaggca ttggtgagca ggaaaaagat cgtgtttacg cgccgttacg
     8161 tttttaacac tattattgca ctaattcgcc ctttgcaatc catcaatgag tagtataaat
     8221 acgctcagtt accttcattc aatctatgga caccaccgtt gaaacgtagt ctgctttttt
     8281 ctgccgtgct gtgtgcggcg tcattgacct ccgtccatgc ggcgcagccg atcactgaac
     8341 cggagtttgc ctctgatatt gtcgatcgtt atgccgatca tattttttac ggcagcggtg
     8401 caacggggat ggcgctggtt gttatcgacg gtaaccagcg cgtctttcgt agttatggcg
     8461 aaacgcgacc tggtaataac gttcgcccgc agctggattc ggtcgtgcgt attgcttccc
     8521 tcaccaaact aatgaccagt gaaatgctgg tgaaattgct cgaccagggg accgtaaagt
     8581 taaacgatcc gctaagtaaa tatgctccgc caggcgcacg cgtgccgacc tacaatggga
     8641 cgcccattac cctagtgaat ctggcaaccc ataccagcgc cctgccccgt gaacagcccg
     8701 gtggcgcggc acatcgtccg gtgtttgtct ggccaacgcg cgagcaacgc tggaaatacc
     8761 tttctacggc gaagctgaaa gccgcgccag gcagtcaggc ggcgtactct aaccttgcgt
     8821 tcgacctgct ggcagatgca ctggcgaatg cgtccggcaa gccttatacc cagttgtttg
     8881 aagagcagat tacccgtcca ttggggatga aagacaccac ctacaccccc tcaccggatc
     8941 agtgccgccg tttaatggtt gctgagcgcg gtgccagtcc gtgcaataac acgctggcgg
     9001 caattggcag cggcggcgtc tattccacgc ctggcgatat gatgcgctgg atgcagcagt
     9061 atttgtcatc tgatttttat cagcgcagta accaggcaga tcgcatgcag acgttgattt
     9121 atcagcgcgc gcagtttacg aaagtgattg gcatggatgt ccccggcaaa gcggatgcgc
     9181 ttggtttagg ctgggtatat atggccccga aagagggtcg tccggggatt attcagaaga
     9241 caggcggtgg tggcggtttc attacctata tggcgatgat cccgcagaag aatattggcg
     9301 cgtttgtggt ggtcactcgt tcgccgttga cgcgctttaa aaatatgagc gatggcatca
     9361 atgatctggt gaccgagcta agcgggaata aaccgttggt tatccccgca tcctgattaa
     9421 tactctgaag gcaaacggtt gatagcgacc agtttgcctt tattcatttc gatatagccg
     9481 cctttacgca aagcagcgag aacttctgcg acgacagaac gcgatatgcg tgtacgttga
     9541 tggatataat tcatcacacc aatacgtgat cgcaattctt cattccagtc aatcattgat
     9601 aataatgttg cgcggatttg ttcgtaggaa ttatgcccaa tgagctgcac gtcgcgtaat
     9661 tccagaattc tattttgcca ggctaaccag taaaaagcgt ctcgccagag ttgattttgt
     9721 tcaataagcg taatggtttg tttggctggt agatgatatc ccgtacaatt tccttctgat
     9781 attaatttgt atggtatatc gtttttcatt aaaccatcag ccagccccat aatataaggg
     9841 gcctgggtaa taccgataag tacgttttct tctctacgca gagagataac tccctctaaa
     9901 ataacgaaag tatcttcgtt attgacatca ctggaaaata taacttgctt ttcattatta
     9961 aactcgaagc gcgtaccgta tctggacaaa catttatcga gcttaccaaa ttcctgaaga
    10021 ggtttaacta cagataacat ttgcgcgtcc tttgcagtaa tgcccgtcaa atccttgacg
    10081 ggcattattt agattaaatt accagtattt cttcggagtg aagaatatta ccaggtatat
    10141 ttaacaccca cgttcgcgga ccagtcttga tctacgtcac caccaccgag gtagttagca
    10201 tcggtatagg cgctgaagtt cttggtgaag ctgaactgag tacccagacc aacacgtacc
    10261 gcagaccctt cagtaccgtt atcgatggaa tcaccgttca catcgttatc gttgttagag
    10321 tcgtcgtaga cgtaagccag tttgaagtac ggagtcagag cctgatcttc gctgtaggtg
    10381 aaggtataac ctgcatctac acccagttca taacgcatgc tgtcgtaaga ctgaccgtca
    10441 actttcatgt cgttgctcag ctggtagtca tcaccagact ggaacagacc agaaacgctg
    10501 ccgtaaggag tcacgtaacc agcatcaccc agtttgaagt cgtaaccggc tttcaagccg
    10561 aagccccaag cgtcggagtt ggtgctaccg tcaacgtaag taccgttgct catggttgca
    10621 gacaggtcgt tgttgaagtg agagtagctc aagctaccat caacaaagac gttgttcgcg
    10681 aagtgagcag aagagtagat gtaggcagtc tggctgtctt gatctacctg accagaacgg
    10741 tcattcatgt cacctttagc gaagcctgca gccgcaccga cgatccactt agcgttgtta
    10801 ccgtcaactt tggtatcaac accgaccatg atgccgttaa catcctgatc atagttgatg
    10861 gtgccgttgt cgccgttgaa gttaccaccg aagtagctta cccatgcgcc gccgttatca
    10921 gccaggccat gacgagagtt ggtcagacga gtaccaacgg tgtcttgttc caggttccag
    10981 atattggtgt tcgcagacgg gatgctcagc gccatgttag cgtagtcggt cagctccatc
    11041 tgttgcagaa caacggtgtt accgcgctgt tcagcctgat aggtgtatgc acccaggtca
    11101 gctttgttag cagcagagaa ggtcgcgttg gttttggtat catcgatata gatgagctgc
    11161 ttacctgcgt aatcagcaat cgcaccagcg ccagtggagt tgtcgatacg aactttgtag
    11221 ttaccggttg cagctacaac gttgtctgca gagctgttgt caacttcgtc gccattgtta
    11281 atgtcgttgt taccattaac ggtcaagtga ccatcagagt tcatcgcaat aacaccataa
    11341 ccgtagttag ctttgctggt gtcgttggta cgatcgttga cgaggtctgc attcaggatg
    11401 taatcattac tatgaatgtc aaacacacct gcgtaaacat taccgttgtt atctttaacg
    11461 ttgccactgg tcagttccag agtatcagtt gcaaatacgc tggagatgtc attcatgttg
    11521 gtgtttacat ccagcacacc accgttagta acggtaatgg tattggcata cagcggtgca
    11581 tatgcccaag ctgaagcagt atcttcaccg aagttaacgg ttgagaaggt atcaatggtc
    11641 aggtgatcgg tgttaacttc accgttaact tccattgaag aaccaccgtt cagagtgatg
    11701 gtgtcagaag tcagagaaga atctgcactc acgttaacct gagaaccgct gttgatcgcc
    11761 agggtatcga tcagggaagt tttcgtggtt tcccaatcag aaccgttgtt cagaacaaca
    11821 tcgaatacgc cgctctggaa tgcgttagcg ttatcaacac gattccagcc atcttcagag
    11881 taagaagcgt acgggttcag gctgtttggt gcaacgtcgt agaaggcttc gtcagcaaca
    11941 acaacgtcat aatcaatatt cgctgaacca acccatttgg tgttgttcag agtcaggttc
    12001 aggtgatcga cgttctgatc gtcagttgcc caaccgccat gagtgtaagc agtggtagag
    12061 ttgtcaatca ggtgacccgt cgcgtcaaag ttagcgttcc aggtactgtc aaatacaacg
    12121 tcaccaagca actgagagtt gttgaacgta acgttgttga ccatacgata atcagaagtg
    12181 tcatcatcga tgaaggctaa cgcaacatca tcagcaggag atgcgccatt gccgtagtca
    12241 cttggttcaa cagaattgcc gaaatgacca cgatctgatt gttcgtcttc agtccatgaa
    12301 ccagaagtta cagtagagtc attaacaacg atgttgttgt cccactgatg accttcggta
    12361 ttgtaggtcg cattaccgag gtcgtgttga accaaagtaa tacctgcaac atgggagttg
    12421 ttgctgatgt tgatgttact ttcaacatcc agagtcacag cggtaccaag accagcggtt
    12481 accaaacgat cataatctgt cttagatgtt ttaccgtctg catccagata agtatcggtg
    12541 aagtagaagg cgtcaaaatc atcgtcaata gtagaattag cgatgttcag agtgaagatg
    12601 tcgccatcaa cccagtcgtg gccagtataa tcgctaccgt tggtccagac gtagtcgcca
    12661 taaccaacct ggtaagatgt aaccatacca tgaacaattg agttggagat gttcagtgta
    12721 ttaccggtat ctgtgccata atcgtggttc agataataag tagaaattac accatttaca
    12781 gtgtcgccgc tgaaagtggt atcaaagtaa ccatcagctg cgtggtcata tacataacca
    12841 ccgtaagtca gataatcgtg atcaatttta gtgctgtcac cccaattgta gatggtggct
    12901 gcgtcatcgt gatacccgaa ggtgttataa gtagctgcat tagcctgaga ggtgatagcc
    12961 agagtgcaag ccaatgctaa ttgtgatact acaagtttct ttttccagga gtgcatttgt
    13021 catccctcct cagggacgta ataagttgat ccatcaatta tcaatgcata gaaaacatat
    13081 tatgtttcgc gctaacgatt atgctgcgtt acagacgaat ggttcaatta ttctttcccc
    13141 taagtccgaa tgtggacata tataatttat ggagaaaaac acgcatcaaa ttgataaaag
    13201 gacgatattt caacatgttg cagataatgt tatttttgat acatacgcgt tataaatatc
    13261 aa
//
LOCUS       DAZPLI010000111        13262 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZPLI010000111 DAZPLI010000000
VERSION     DAZPLI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.112"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="ACHUU3_003020"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="ACHUU3_003020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR2083031.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHUU3_003021"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHUU3_003021"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR2083032.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHUU3_003022"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHUU3_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR2083033.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="ACHUU3_003023"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="ACHUU3_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR2083034.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="ACHUU3_003024"
     CDS             complement(4036..5568)
                     /locus_tag="ACHUU3_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR2083035.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="ACHUU3_003025"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="ACHUU3_003025"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR2083036.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="ACHUU3_003026"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="ACHUU3_003026"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR2083037.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="ACHUU3_003027"
     CDS             complement(8663..10399)
                     /locus_tag="ACHUU3_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR2083038.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSASWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHUU3_003028"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHUU3_003028"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR2083039.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="ACHUU3_003029"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="ACHUU3_003029"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR2083040.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="ACHUU3_003030"
     CDS             complement(12121..12855)
                     /locus_tag="ACHUU3_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR2083041.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13262
                     /locus_tag="ACHUU3_003031"
     CDS             13056..>13262
                     /locus_tag="ACHUU3_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR2083042.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVV"
BASE COUNT         3366 a         3371 c         3489 g         3036 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 accgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgagg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatccggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc cgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact ggcactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacta
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
    13261 tg
//
LOCUS       DAZPLI010000112        13210 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZPLI010000112 DAZPLI010000000
VERSION     DAZPLI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13210
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.113"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..807
                     /locus_tag="ACHUU3_003032"
     CDS             <1..807
                     /locus_tag="ACHUU3_003032"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR2083043.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            1177..2001
                     /gene="gadX"
                     /locus_tag="ACHUU3_003033"
     CDS             1177..2001
                     /gene="gadX"
                     /locus_tag="ACHUU3_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR2083044.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            2369..3097
                     /gene="gadW"
                     /locus_tag="ACHUU3_003034"
     CDS             2369..3097
                     /gene="gadW"
                     /locus_tag="ACHUU3_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR2083045.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(3460..6573)
                     /gene="mdtF"
                     /locus_tag="ACHUU3_003035"
     CDS             complement(3460..6573)
                     /gene="mdtF"
                     /locus_tag="ACHUU3_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFR2083046.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(6598..7707)
                     /gene="mdtE"
                     /locus_tag="ACHUU3_003036"
     CDS             complement(6598..7707)
                     /gene="mdtE"
                     /locus_tag="ACHUU3_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFR2083047.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7815..8093
                     /locus_tag="ACHUU3_003037"
     CDS             7815..8093
                     /locus_tag="ACHUU3_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083048.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(8094..8621)
                     /gene="gadE"
                     /locus_tag="ACHUU3_003038"
     CDS             complement(8094..8621)
                     /gene="gadE"
                     /locus_tag="ACHUU3_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR2083049.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(9420..9992)
                     /gene="hdeD"
                     /locus_tag="ACHUU3_003039"
     CDS             complement(9420..9992)
                     /gene="hdeD"
                     /locus_tag="ACHUU3_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR2083050.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            10247..10579
                     /gene="hdeA"
                     /locus_tag="ACHUU3_003040"
     CDS             10247..10579
                     /gene="hdeA"
                     /locus_tag="ACHUU3_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR2083051.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10695..11021
                     /gene="hdeB"
                     /locus_tag="ACHUU3_003041"
     CDS             10695..11021
                     /gene="hdeB"
                     /locus_tag="ACHUU3_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR2083052.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            11085..11732
                     /locus_tag="ACHUU3_003042"
     CDS             11085..11732
                     /locus_tag="ACHUU3_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFR2083053.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(11774..12304)
                     /locus_tag="ACHUU3_003043"
     CDS             complement(11774..12304)
                     /locus_tag="ACHUU3_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR2083054.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(12460..13026)
                     /gene="slp"
                     /locus_tag="ACHUU3_003044"
     CDS             complement(12460..13026)
                     /gene="slp"
                     /locus_tag="ACHUU3_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR2083055.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3599 a         2983 c         3030 g         3598 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg
      721 gaagactaca aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc
      781 cagcagaaca gctttaaaca cacctgataa cataacgttg taaaaaccga atgcccagcc
      841 tttaaaaaaa cagctgggca ttcggttgct tattaatcgc aataatatat tggctgtcta
      901 ttcatcgtgt tgataagata tatgttatat gaatgtttat taaatgtagc gattgccttt
      961 aatgtcacga aaagggatta tttgcttgct attaatttca ctgcgttctt aaacacattt
     1021 ttgtattata aatccagtca tcctgcccgt agaacatcac acattatcat cctgttctcc
     1081 cgcttcgttt aaatttattt atcaatcaat ttgacttaag agggcggcgt gctacattaa
     1141 ttaacagtaa tatgtttatg taatattaag tcaactatgc aatcattaca tgggaattgt
     1201 ctaattgcgt acgcaagaca taaatatatt ctcaccatgg ttaatggtga atatcgctat
     1261 tttaatggcg gtgacctggt ttttgcggat gcaagccaaa ttcgagtaga taagtgtgtt
     1321 gaaaattttg tattagtatc aagggatacg ctttcattat ttctgccgat gctcaaggag
     1381 gaggcattaa atcttcatgc acataaaaaa atttcttcat tactcgttca tcactgtagc
     1441 agagatattc ctgtttttca ggaagttgcg caactatcgc agaataagaa tcttcgctat
     1501 gcagaaatgc tacgtaaaag agcattaatc tttgcgttgt tatctgtttt tcttgaggat
     1561 gagcacttta taccgctgct tttgaacgtt ttgcaaccga acatgcgaac acgagtttgt
     1621 acggttatca ataataatat cgcccatgag tggacactag cccgaatcgc cagcgagctg
     1681 ttgatgagtc caagcctgtt aaagaaaaaa ttgcgcgaag aagagacatc atattcacag
     1741 ttgcttactg agtgtagaat gcaacgtgct ttgcaactta ttgttataca tggcttttca
     1801 attaagcgag tcgcagtgtc ctgtggatat cacagcgtgt cgtatttcat ttacgtcttt
     1861 cgaaattatt atgggatgac gcccacagag tatcaggagc gatcggcgca gggattgccg
     1921 aaccgtgact cggcggcaag tattgttgcg caaggaaatt tttacggcac tgaccgttct
     1981 gcggaaggaa taagattata gaattttact cagacataaa aaaaacccgg cataggggac
     2041 cgggaagagg atagtctgcc gtctccagac taataaaccg ttataacact ccctgttggc
     2101 acgggaaact ttgtgctctc agtaagttaa atataacttt tactggaaat aagatcagcc
     2161 atttttttat aaacataagc tatacgctgt gcgaaaatat attcgtgctg catttactta
     2221 ttatcaatta actgttatgc aaaactactt tgtggataaa ttttggtcct accaaatctg
     2281 gcagtttttt cgctaagaaa cagtctggca acatttcatt agtatactga aattgaaata
     2341 atcgcagtat gaaatataag ggataatcat gactcatgtc tgctcggtga tcctcattcg
     2401 tcgttcattc gatatttatc atgaacagca taaaatatcg ctgcataacg agagtatcgt
     2461 gctgctggag aaaaatttgg cagacgattt tgcgttttgt tcaccggata cgcgacgact
     2521 ggatatcgat gagctgacag tttgccatta cttacaaaat attcgtcagc taccacgcaa
     2581 tttagggtta catagcaaag accgtctatt aattaaccag tcacccccca tgccgctggt
     2641 gacggcgatt tttgatagct tcaatgaatc cggggtaaat tcaccgatac tgagcaatat
     2701 gctctacctt tcctgtttat cgatgttttc tcataagaaa gaactgatcc ccttactttt
     2761 caatagcatc agcactgttt caggaaaagt tgaacgcctt attagctttg atatcgccaa
     2821 acgttggtat ctgcgcgata tcgcggaaag aatgtatacc agcgagagtc tcataaaaaa
     2881 aaagttgcag gatgaaaata cctgtttcag taaaatatta ctcgcttcca ggatgtcgat
     2941 ggccagacga ttactcgagt tacgtcaaat tcctctgcat actattgcag aaaaatgtgg
     3001 ctatagcagt acgtcgtact ttataaacac atttcgacaa tattatggtg taacgccaca
     3061 tcagtttgcg caacattcgc caggtacctt ttcctgacat attttgcatt tgaatattgg
     3121 tcaggatctc acacctgctt catgtgaaac tcttccctga tgatttctgc cgggctaccg
     3181 gctagttctc tttcgcagtt tgccaggcgg tataggtatt ttcaggagga aaagactcgg
     3241 catgtttggg attattaagc tgacaattca tacgattacg ggaatgtggg tcagtattgt
     3301 gctgttcaaa ttgatgacga atggctggag tggtttctac tttcagtgct gtgtactaag
     3361 cctggtattc ctgacggtta gctggttgtt gagtggtgaa tggttagcag gaaagagtaa
     3421 ggctgaacct tcacgttcaa ccttactctc atttacacgt tacgcttttt taaagcgggc
     3481 aaagagatgt tccactacaa cgaaaaagac cggaacgaag taaattgcca gcactgttgc
     3541 ggcaaacatc ccgcccatca cgccggtacc taccgcgttt tgcgcgccag aaccggcacc
     3601 atgactgata accagcggca gcacgccgag aataaaggcg agagaggtca tcaggattgg
     3661 gcgtaaacgc atccgcgccg cttcgatgat tgcctctatc ggcgttttcc cttctttctg
     3721 catcatctca acggcaaatt cgacaatcag gatggcgttc ttggcggaaa gcccgatggt
     3781 ggtcagcaaa ccaacctgga agtagacgtc attacttaag ccgcgcagat cggtggccag
     3841 taatgcgcca acgacgccta acggaacaac caacatcacc gagaacggaa ttgaccagct
     3901 ctcatagagt gcggcgaggg cgaggaacac cacgaccagt gaaatggcat acagtgcagg
     3961 agcctggttt gaggataacg cttcctgata cgacagtccg gtccatgagt agccgacgcc
     4021 tgccggaagt ttagcgacca ggtctgccat aaatttcata gcgtcaccgg tacttttccc
     4081 ggccgccgct tcacctaaaa tctccattga cgggatgccg ttgtagcgtt ccaatcgcgg
     4141 tgaaccatag gtccattcgg tagacgagta ggcagaaagc ggtgccatcg tgccagaggc
     4201 gttgcgtaca taccattggt tgatgttatc cggcaacata cggaacggcg tgcctgcctg
     4261 gacatacact tttttcaccc gcccctggtt gaggaagtcg ttcacgtagc tgctgccgaa
     4321 ggcggtggaa attgtctggt tgatatcaga cagcgccaca cccattgcct cagctttcgc
     4381 ggcgttgacg ttcactttga acatcggcgt atcttccagg ccgttcgggc gtaccccggt
     4441 gacctggtca ggcgattgcg ctgccagtga taacagctcg tttcgtgcct gggttagttt
     4501 ttcgtgcccc aggttaccgt tgtccagcag ttccatatca aaacctgacg cggtacccag
     4561 ttcagccacc gcgggtaagt tgaacgggaa gacgacggct ttattgatac tgcttaacgc
     4621 aatcattgcc cgctgaatga tcgcggtaac cgagttttcc tcaccgacac gttcagacca
     4681 cggcttgaga ctgataaacg ccaggccgtt gttttgcccc tggccgctga agccaaagcc
     4741 gccaacggta aacaccgact gaacattatc tttctctttt gtaagataat aatccgtcac
     4801 ctgttgcagc actttcgtgg tgttaaccat ggtggcaccg gaaggtaact gcgcggtggt
     4861 cataaatacc ccctgatcct cttctggtaa gaaagaggtc ggtgtacgca ggaacagcac
     4921 cgccatcccg gcgcaaatca gcaggtagac caccatgtag cgaccggtac aacgcaacag
     4981 cgagcgggtg ctatcggtat agtgctgagt tgatttttca aacagcgtgt tgaagcgtgc
     5041 gaacagggcg ttaggtttgt gaccgccttc cggcgcggct ttcagaatgg tggcgcacag
     5101 ggcaggggtc aggctcattg ccacaaatac tgaaagcagc atggaggaga tcagcgtgat
     5161 ggagaactgg cggtagatct ccccggttgc gccgctcata aaggccatcg gcataaacac
     5221 tgcggaaaga acaacagcaa taccgaccag cgcacgttgg atctgcccca tcgatttatg
     5281 cgtcgcttcc ttcggtggta gcttatcttc cgcaatgaca cgctcgacgt tctccaccac
     5341 cacgatggcg tcatccacca gtaaccctat cgccagcacc atcccgaaca tagttaacgt
     5401 gttgatggtg aaaccgaccg ccgacaagat cgcaaacgtc ccgagaataa ccaccggtac
     5461 ggcaatcgtc gggatgattg tggcacggaa attctgcaaa aacagataca tgaccaggaa
     5521 gacgaggatg atagcctcaa ccagtgtttt aaaaacttcc tgaatagaaa tttcgataaa
     5581 cggcgtggtg tcgtaaggat aaaccgtctt cagacttgcg gggaaatagg ctgataagcg
     5641 gttcagctcc tctttgactg cccgcgaggt atccagggcg tttgctccgg tagccagttt
     5701 gatggcgatc ccggcagcag gtttgccgtt atagcgcgcc acggtggaat aatcttccgc
     5761 tccaagttcg acgcgggcga catcacgcag cagcacttgc gaaccatctt gctgaacttt
     5821 caacaggatt ttgccaaatt cttccggcgt ttgcagacgc gtctgcacaa tgatcgaggc
     5881 gtttagttgc tggtctgccg cctgtggcat gccacccagt tgaccaccgg aaatctggtt
     5941 gttttgcacc ttaatctggg aaataacatc ggaaggtacc agattgtatt tattgagttt
     6001 ttgcgggtcc agccagatac gcatggcgta ctcggaacca aagagctgta cgctaccaac
     6061 gcccgcggta cggcttagcg ggtctttgat attagacgct acatagtccg cgatatcgta
     6121 ctggttgagg ctgccgttat cagaaataaa cgccgctacc atcagaatat tactgctcga
     6181 cttatcaacg ctaatcccct gctgctgcac cgcttcaggt aatgaaggca tagcgagttg
     6241 cagtttattt tgcacctgaa cctgtgcgat atcaggagat gtcccagtct cgaaggtcag
     6301 agtgatagag gcattacccg ccgcatcact ggttgaagac atgtacatca ggccatcaag
     6361 cccattcata ttttgctcaa tcacctgagt gaccgagtct tctaccgttt gcgcatcggc
     6421 acctggatag gtagcgctga cggtaatggt cggtggcgca atctgcggat actgcgcaac
     6481 cggtaagttc atgatcgcca gaccacctgc aagcatcata ataatggcaa gtacccaggc
     6541 aaaaaccggg cgatcaataa aatagttagc catgaaagtc cccttaagcc tgcaacgtta
     6601 ttgtttcgat tcggtgctgg cgttttcctg gctggaggaa attgctcgtg ctttgatacc
     6661 cggacgaatg cgttgcaaac cggaaacgat cacccgatcg ccagcctgca agccagaggt
     6721 gacaacccac tggtcgccga tggctttgct ggcttcaatt tcgcgtagct gcacaacatc
     6781 gtctttatcc agaatgagcg ccgttgcttt accctgggcg ttgtgagtga cgccttcctg
     6841 cggcaccagt aatacattct ggcggctacc ttcatccact aatgccgtga cgtacatgcc
     6901 aggcagcaag tcaccatttg ggttggggaa aatcgcccgt aacgtcacgg agcccgtggt
     6961 ttcatccacc gtcgggtcgg agaatttcag tgtgccggtc tggctgtagc gtttaccatt
     7021 ttccagattg agctgtactg gcgtactgcc ctgaacctgt ttgatttgcc cactggcgac
     7081 ctcttctttc atgcgcaaga aatcctgcac cgactgcgtg agatcgacat aaatcgggtc
     7141 cagacgttgt acggtaacca gcgaatctgc ctgattagcg gtaacgagtg cgccgacggt
     7201 caccgacgat ttcccgctga cgcccgtaat cggcgaggtg acattcgcgt attgcagatt
     7261 gatcgtcgcc tgttcaacag ccgctttggc gacggtgaca ttggcttctg cttcattcag
     7321 ctgggtgcgc gcggtgtcgt aatcctgacg gctaacgtag ttggtcttca gcaacgatgc
     7381 ctggcggtta aaggtgatgc gggcattgct ggcggtagag agcgctttcg ccagcgagcc
     7441 tttggcggag tttagctcgg cctgtaaagg tgcaggatca atctgataca gcgaatcgcc
     7501 ctggttcact ttatcgcctt cgataaagtt gcgtttaatg ataataccgc ccacctgggg
     7561 acgtatctcg gcaacttcat aaggaacggt tctaccgggc aattcgctca acacattgac
     7621 cgaaccgggg gagagtgtga cgacaccgac ctcaggcgtc atggcggcgg cgttttccgc
     7681 cgatttgtca tcgcaggcgg tgagcatcgc gccgcagaat aacaacggta ttaacagctt
     7741 tcttcttctg ttcattttag tccctgaaaa ttcttgagaa ataacggttc tttaaattgc
     7801 gcgcgaggag agatatgagg gatttacaga ccagcggcat agtggggttg tcagccagta
     7861 aggtgggata caggcacagt gatcgacatg gtgaggtcaa cgacaaaaaa aatgttgccg
     7921 ttctgccaac agttcctgcc agcattcggg caacgaaggg ttctactggt ggatacacat
     7981 acctggggaa taaagtcgtt ttagcctctc ctttagtaat caccgggggt aatgaaatca
     8041 gcatctgcat ccctcgtcat gccagccatc aatttcagtt gcttatgtcc tgactaaaaa
     8101 taagatgtga tacccagggt gacgatgtcg ctcatacgtc ttagtttcag cttgtacata
     8161 atgttgaatt tctgaatctt taatgtttgt tgcgaaatat tgagcgtcga cgtgatattg
     8221 ctttcatttt tatttttcag aatcagttgc ataattttga actgattatg tgaaaaacat
     8281 aattgtgaat tcttatgggg caagtgttta ccataagtaa tctcaagaag atttttgata
     8341 ctttctttgc ggctaacaaa ataaataggc gctttagctt ttagtttact gatgtgataa
     8401 ggtgccaaaa caataattct ctcggcatct aatttctcca gaaatttaat cgcttcttca
     8461 tcaaggatat gattgtgata ggtatcaatg ataaccttga agggtttatt gcctactttt
     8521 ttgatctctg acagggaatt gatttttatc atttcgtgat tatctttcaa ctgccaaaag
     8581 ccctgtaaaa gaaaagaatc tttcgtcatg agaaaaatca taacttgctc cttagccgtt
     8641 atcgtttcga acatgtcatc cttttcatta tttacatcct tgtccgaatc gttgttcaat
     8701 atagtaaacg cctataagaa ctttaccctt cgtgaactaa tacgtctatt aacactaatc
     8761 aatacttcag atttatccat cacgcactcc atatattgat cctaagtaag attcctggtt
     8821 gttatcagct tgtaaatagt aaacaaaaag ttaacgtttg cggattactg cccaagaata
     8881 agttatcaat aaccagtttg tgatctctga agaatattac taaagttaaa atgtaatccg
     8941 atttaaatat cgagtctcct tgtttcgact taagctggca attggattgc cagctttctt
     9001 ttaacgcatt gaaattacct gagtaaataa cgcattcatg tcttcaggtt tttatatagc
     9061 gtctggagac acggatattt atgcaatgta atcaacgcca gttgcaaaaa aatgaacccg
     9121 gattacttat ttaaccgccc atagcatcaa cgcttacgga actctttgtt cactttccca
     9181 cagcactttt aaaacattaa acctgactac ggaaaatatc agccatgata atgtttgaaa
     9241 tggctaattt gccatggagt gaaaaaaatt agatgaaatt cagtaggttg aaataatcac
     9301 tagcaggtaa ttatttcaat gatagtgcgc aattgatcta caacactccg tagcggagag
     9361 agtattaatc ggatcatagt catatcaagt gactatgatc cgggtgacaa ccgggataat
     9421 tattgctgct taacgaacaa actggcgaag ctgaacaggc tggcggcgct aaatatcagt
     9481 tcaattccca ccagtgtgga aaccagcgtt acagacacca tcggcgttgc accaaggaat
     9541 atccaggcaa tgacgatatc cagcacacca ataacgagct gtagccagct gcctttcatt
     9601 gaacgctgac gataccaact catcaggcga ataacccctg caacacagaa caaaccggca
     9661 ataaatgccg caatggcaaa aatgcccagc tccggtgcgc ggatgaagaa atagccgatc
     9721 aataaatagg cgactgcgac gaggaaaccg gataataccg gccagaaatt atgactgcgg
     9781 ttgctgaata acccgacaat aagcgcaatg cccgagcaga ttaataatgc acccactact
     9841 gtgcttaaaa tatcgccaga gacgaacggg aaactgatac acagcaaccc gacgataaac
     9901 agtagcacgg caataaactg gattgctctg cgatgttttt taagcatctc cagatcaaac
     9961 ttcaaaattg ttgccttatc tatatataac atagaaccac cctataaaat taagaagaaa
    10021 atcccctgct atcaatctat gccaaaaacg cgtctaagaa tgtagtcgat ttaataaaaa
    10081 tttcctaatt acagtatctg atgcatctgt aactcattgt attgaaataa aaatatctga
    10141 ttttgatatt ttatatcaac atgacatata cagaaaacca ggttataacc tcagtgtcga
    10201 aattgattcg tgacggctct ttcactttat agttgaggat attacgatga aaaaagtatt
    10261 aggcgttatt cttggtggtc tgcttcttct gccagttgtg agcaatgcag cggatgcgca
    10321 aaaagcagct gataacaaaa aaccggtcaa ctcctggacc tgtgaagatt tcctggctgt
    10381 ggacgaatcc ttccagccaa ctgcagttgg ttttgctgaa gcgctgaaca acaaagataa
    10441 accagaagat gcggttttag atgttcaggg tattgcaacc gtaaccccag ctatcgttca
    10501 ggcttgtact caggataaac aagccaactt taaagataaa gttaaaggcg aatgggacaa
    10561 aattaagaaa gatatgtaat tccgggaatg cgttacatcg tacttccttg catattgaac
    10621 aggccggaat atcttcttta aaagcagcta ttcctcctgt tcatatataa tctctatatt
    10681 gaatgggtta caaaatgaat atttcatctc tccgtaaagc gtttattttt atgggcgctg
    10741 tagcggcttt gtcactggtg aacgcacaat ctgcgctggc agccaatgaa tccgctaaag
    10801 atatgacctg ccaggaattt attgatctga atccaaaagc aatgaccccg gttgcatggt
    10861 ggatgctgca tgaagaaaca gtatataaag gtggcgatac cgttacttta aatgaaaccg
    10921 atctcactca aattcctaaa gtgatcgaat actgtaagaa aaacccgcag aaaaatttgt
    10981 ataccttcaa aaatcaagca tctaatgact tgccgaatta atgaggtaca agtaaaaagg
    11041 agtagcaagt tgagccatct tgctgctcct ttttgcattt ttatatgaca gcagaattta
    11101 ttatacgtct tatactcgcg gcaattgcct gtggcgctat tggcatggaa aggcaaatgc
    11161 gcggcaaagg agcagggtta cgcacacatg tattaattgg catgggaagc gccctgttta
    11221 tgattgtttc gaaatatggt tttgctgacg tgctgtcttt agatcacgtc ggactcgacc
    11281 ccagccgtat tgctgctcag gtggtgacgg gcgtcgggtt tatcggtgca ggtaacattc
    11341 tcgttcgtaa ccaaaatatt gtaggtctga cgacggcagc ggatatctgg gtgaccgccg
    11401 ccataggtat ggttattggc agcggtatgt acgaactggg tatttatggt tcagtgatga
    11461 ccttgttggt gctggaagtc ttccatcaat taaccttccg cctgatgaac aaaaattacc
    11521 atttacagct gacattagtg aacggtaata tggtatcaat gctcgactgg tttaagcagc
    11581 aaaaaataaa aactgattta gtttcgttgc aggaaaatga agatcatgaa gtggtcgcca
    11641 ttgatatcca gcttcatgcc acaacgtcga tagaagacct gttgcgatta ttaaaaggta
    11701 tggcaggggt gaaaggcgtt tcaattagct aatctgtgtg gcagcgtagc cagactcacc
    11761 gtaagcctga aattcacacc agataatcaa tatgctgatg gcgtaccagc tcattgatcc
    11821 gcttaagatt gaggatcatc atgatattgt gtttatggca gtagaacgtt tttagcgata
    11881 ttttcagttt ctccagaata tcttcagttg tcatttgatt cgcaatgcag ctcagaacct
    11941 gatgttgctt gcgcgaaagt gacacattcg cttcaggcac agagcagtaa ctgccattga
    12001 tgagtgcgac aaaatcaatg atagtgatag tccgcggaac aaaggtcacc ggtactttcc
    12061 ccagacagcg tttaatatta aagggggaga aaacaatgac atggacaggt tttaatcgtt
    12121 caatttgagt gagaaaatta gaatgaaata cattattcat taatgtatca ataatgacga
    12181 aggcattctg atgcaacatt acgtcgatct cttcttcgtt ctgtatatga acgacattac
    12241 ctttactcag aatgtttttc atcgcggtga agaacatcgt atccctggta attataagaa
    12301 acataaccga ctcctggtgc ggacattaac tttataatca atttgccaaa ggtaattaac
    12361 aggacagggc ttataataat aatagttagc ttactgtata ttaattttat gtaatacatg
    12421 atgcagatgc tgagaaagca tctgcatctt tcggtggtgt tatttgacca gctcaggtgt
    12481 tacctgactc accgcattgg tgtagtaagg cgcaccccag cccggttccg gccagaatgc
    12541 accatagccg taatcccaca ggttataggt ggtattaacc acttctctca aatgccacac
    12601 ctggatgccc tgcatattca cttccaggaa gttatacggg actttattga taaagccagg
    12661 ttgttcaccc tgaatggtac cgaggatggt aacaaagtga ttacgatagt tcactgggtc
    12721 aaggaagccg ctttgtctgg cgagcagtcg gccctgatag ttggcttcaa tatcaggttt
    12781 cgcatagcta tccaacggta atacagcgat ttctaacaac gtatccgttt tgccattgat
    12841 aacgttgata accttcccac caaagcgcgc ttgttgacca acatataacc ccggctggtt
    12901 atgaacagca acaaaacttt tttgaatatc aggttggtta ttgcctttga tattttgcgg
    12961 aattgaacta catgcggcaa gcaaaaatga aaggctgagg atgagtgcac cttttgtcat
    13021 gttcatgtta ctatccttat caacaaatat taaaaccata atagatattt ctttatagtt
    13081 ttcatctgat tctgaggcga tatgcctgat agtaacgtaa acctgtctga tgtttatatc
    13141 ccttttgttt agttcaattc cattgatttt ggggcgtggc atgagtatcc atcgccccat
    13201 aatataatcc
//
LOCUS       DAZPLI010000113        13123 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZPLI010000113 DAZPLI010000000
VERSION     DAZPLI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13123
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.114"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            633..1379
                     /gene="mipA"
                     /locus_tag="ACHUU3_003045"
     CDS             633..1379
                     /gene="mipA"
                     /locus_tag="ACHUU3_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR2083056.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            1469..2323
                     /gene="yeaE"
                     /locus_tag="ACHUU3_003046"
     CDS             1469..2323
                     /gene="yeaE"
                     /locus_tag="ACHUU3_003046"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFR2083057.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(2374..3258)
                     /locus_tag="ACHUU3_003047"
     CDS             complement(2374..3258)
                     /locus_tag="ACHUU3_003047"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR2083058.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(3342..4337)
                     /gene="gapA"
                     /locus_tag="ACHUU3_003048"
     CDS             complement(3342..4337)
                     /gene="gapA"
                     /locus_tag="ACHUU3_003048"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR2083059.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            4679..5092
                     /gene="msrB"
                     /locus_tag="ACHUU3_003049"
     CDS             4679..5092
                     /gene="msrB"
                     /locus_tag="ACHUU3_003049"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR2083060.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            5134..5406
                     /locus_tag="ACHUU3_003050"
     CDS             5134..5406
                     /locus_tag="ACHUU3_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR2083061.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(5567..6208)
                     /gene="pncA"
                     /locus_tag="ACHUU3_003051"
     CDS             complement(5567..6208)
                     /gene="pncA"
                     /locus_tag="ACHUU3_003051"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR2083062.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(6219..7235)
                     /gene="ansA"
                     /locus_tag="ACHUU3_003052"
     CDS             complement(6219..7235)
                     /gene="ansA"
                     /locus_tag="ACHUU3_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR2083063.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(7402..9258)
                     /gene="sppA"
                     /locus_tag="ACHUU3_003053"
     CDS             complement(7402..9258)
                     /gene="sppA"
                     /locus_tag="ACHUU3_003053"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR2083064.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            9419..9970
                     /locus_tag="ACHUU3_003054"
     CDS             9419..9970
                     /locus_tag="ACHUU3_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR2083065.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            10087..11130
                     /gene="selD"
                     /locus_tag="ACHUU3_003055"
     CDS             10087..11130
                     /gene="selD"
                     /locus_tag="ACHUU3_003055"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR2083066.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            11135..13096
                     /gene="topB"
                     /locus_tag="ACHUU3_003056"
     CDS             11135..13096
                     /gene="topB"
                     /locus_tag="ACHUU3_003056"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR2083067.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         3309 a         3220 c         3448 g         3146 t
ORIGIN      
        1 accagaagtt tgtgaaggat actggctatg agctacatcc aggtgtaggc atcgatacaa
       61 gtatctacag ggatgaatgg aatggtacta aggtgatatg cgaatcggtg ttagtagatg
      121 atgacggtac aaatctgtaa ctaaattagt taagaaatca taaagatagt agtacttgca
      181 aatgttccca ttgatgaagt tatgcgtcct ttcgtttttt agcacaggtt aagagccgtt
      241 catatgaata gtagaggctc ccggaagagg taaactgaac gacgtctctt gctaaaacac
      301 agcaactctc atgccatttt atcgacgatg agaacaacgt gcccggtgat acaccttcaa
      361 aattaagcgt agatggcaaa tggaaaactt gcctgcacgg ctttcttaat ttcaatggca
      421 tatcaacaac tcatccataa attttgcata attaatgtaa agacaaggct cgccagtaac
      481 gctaaattca tttggctgta agcgcggtgt catccgcgtc aggaaaatta aacagttact
      541 ttaaaaaatg aaaacgtaaa aaggttgggt ttcgatgtat tgacgggtaa actttgtcgc
      601 ccgctaaaca tttgtttaag gaatgattaa ttgtgaccaa actcaaactt ctggcacttg
      661 gagtgcttat cgcaacgtct gcaggcgtag cgcacgctga aggtaaattt tccctgggcg
      721 caggcgtcgg tgtcgttgaa cacccatata aagattacga taccgatgtt tacccagtac
      781 cggtaatcaa ctatgaaggc gataacttct ggttccgtgg cttaggtggt ggttactacc
      841 tgtggaatga cgcaacggac aaactttcaa ttaccgctta ttggtcgccg ctttacttca
      901 aagcgaaaga cagtggcgat caccaaatgc gtcacctgga tgaccgtaag agcaccatga
      961 tggctggtct gtcttatgct cactttaccc agtacggtta cctgcgtacc accctggctg
     1021 gcgataccct ggataacagc aacggcattg tctgggatat ggcctggttg tatcgttaca
     1081 ccaacggcgg cctgacagtg accccgggta tcggtgtgca gtggaacagc gaaaaccaga
     1141 acgaatacta ttatggcgta tcgcgcaaag agtccgctcg cagcggtctg cgtggctata
     1201 acccgaacga cagctggagc ccttacctgg agctgagcgc cagctacaac ttcctcggcg
     1261 actggagtgt ttacggtacc gcgcgctata cccgtctgtc tgatgaagtt actgacagcc
     1321 cgatggtgga taaatcctgg actggcctga tttctaccgg gatcacctac aaattctgat
     1381 aatgcatctt tccagggcat tttgtgcata aaaacagggc gtcacgccct gttttgcatt
     1441 acggtggtgc aatacctggg aggaaacaat gcaacaaaaa atgattcaat ttagtggcga
     1501 tgtctcactg ccagccatag ggcagggaac atggtatatg ggcgaagatg ccagtcagcg
     1561 caaaacagaa gttgctgcac tacgcgcggg cattgagctc ggtttaaccc tcattgatac
     1621 cgccgaaatg tatgccgatg gcggtgccga aaaggtggtt ggggaagcat taaccggtct
     1681 gcgtgataac gtctttctcg tctctaaagt ctatccgtgg aatgctggcg ggcaaaaagc
     1741 gataaatgca tgcgaagcca gtttacgccg tctcaatact gattatctcg atctctactt
     1801 attacacagg tctggcagtt tcgcttttga agagactgtt gcagcgatgg aaaaattgat
     1861 cgcccaggga aaaatccgcc gctggggcgt ttctaacctt gattatgctg atatgcagga
     1921 actctggcag ctgccggggg gaaatcagtg tgccactaat caggtgcttt accatctcgg
     1981 ttcacgaggg attgagtacg atctactccc ctggtgccag caacagcaga tgccggtgat
     2041 ggcttacagt ccattagccc aggccgggcg gttgcgcaat ggactgttaa aaaacgcggt
     2101 agtcaacgaa attgcacatg ctcacaatat cagctcggca caagtattgt tggcgtgggt
     2161 aatcagccat cagggtgtga tggcgattcc aaaagcggcc acgattgccc atgttcaaca
     2221 gaatgcggct gtgcttgagg tcgaactttc gtcagcggaa ttaaccatgc tggataaagc
     2281 gtatccggca ccaaaaggaa aaactgcgct ggatatggtg tgatgttgat acgtgccgga
     2341 tgcatcgcac ccggcacaca acattaacgc aaattaacgt ttcgcaacgc gaatggattg
     2401 cgccagatgt gcaggtttct ctttggtgac tttttgcgtt tctgaagcgt aagccgtttc
     2461 tacacaaaca aatgttttgt agccatcatc cggcatatcg cccatgctaa ttgaaagcgc
     2521 cggtcccggg ttccagccta caacgttcag atgatgctgg tggcctacgg cgataatacg
     2581 attcagcgct tcatcattaa tcacgctgca atcttgcgga ttcagataca cgcgatcggt
     2641 acggtcaggg aaggtctgaa taccgtcggt cagtacatct tctttcgcgt cattcacttt
     2701 atcaatgaag cgatcgccca gcccactgac gcttacctta gcgatatcac ccacgttaaa
     2761 gtaggtatgc agggcagagg tggtttcaaa ttcgccatgt gattcaagat cgatttcaca
     2821 ggttttaccc acgcggaaat gcgctaacag cgtaaagtcg tgcggacaga attttttcgt
     2881 ctcttcgctt tgcgtcaatt caaaagtcag cgctacgcca tcagcatctt cattatgtga
     2941 tttcagcgtc cacggcaggt tgcgggcaaa accgtgcgca ggcagacctt gttgtgccgc
     3001 cggaccaaac cacggccagc aaaccggtac gccaccgcga atagcgacgc catttttgaa
     3061 cggtgtgttg ttgctcaacc acagaacttc ttcttcaccc gcaggtttcc acgagagaag
     3121 gtgtgcgccc tgtaatgcaa aagaggcttt tacttgggga tgatcgacca caatgaggtc
     3181 cagttcatcc agtttacgac gggagaggac aggggagatt tgttcgatga ccggaagggc
     3241 aaaaattttc ttaatcatga cgcagtcctt taacttcatt ttatcaggta aaaaaaagag
     3301 cgaccgaagt cgctcttttt agatcacagt ttcatctcaa cttatttgga gatgtgagcg
     3361 atcaggtcca gaactttgtt ggagtaaccg gtttcgttgt cgtaccagga taccagtttc
     3421 acgaagttgt cgttcagagc gataccagct ttagcatcga acacggaagt gcaaacttcg
     3481 ccgttgaaat cggtagatac tacgtcatct tcggtgtagc ccagaacgcc tttcatttcg
     3541 ccttcagcag cggctttaac ggcagctttg atctgctcgt aagttgcagc tttttccaga
     3601 cgaacggtca ggtcaactac ggatacgttc ggggtcggaa cgcggaacgc cataccagtc
     3661 agtttgccat tcagttctgg cagtacttta cctacagctt tagcagcacc ggtagaggac
     3721 gggatgatgt tctgggaagc gccgcggccg ccgcgccagt ctttgtgaga cgggccatca
     3781 acggttttct gagtagcggt agtagcgtga acggtggtca tcagaccttc gatgatgccg
     3841 aagttatcgt tgataacttt agccagcgga gccaggcagt tggtggtgca ggaagcgttg
     3901 gaaacgatgt cctggccagc atatttgtcg aagttagcgc ctttaacgaa catcggagtg
     3961 ttgtctttag acggaccagt cataaccact ttcttcgcac cagcggtgat gtgtttacga
     4021 gcagtttcgt cagtcaggaa cagaccagtt gcttcagcga caacgtcaac accaacttcg
     4081 tcccatttca ggttagccgg atcacgttca gcggtaacac ggattttttt accgttaacg
     4141 atcagatgac cgtctttcac ttcaacggta ccgtcgaaac ggccgtgagt ggagtcatat
     4201 ttcagcatgt atgccatgta atcagcgtct aacaggtcgt tgattgcaac gatctcgatg
     4261 tcagaacgtt tctgagcagc acggaaaaca atgcgaccga tacggccaaa accgttgata
     4321 cctactttga tagtcatata ttccaccagc tatttgttag tgaataaaag gttgcctgta
     4381 aaattacaaa aaccttacgc agcgtcaagc ggaatcgtgt caatcattgc gacaaatcaa
     4441 tcctgtgcct aagcattacg cgactgactc gcctcactct tcctttgggc ttgaaaccac
     4501 atggggtcgg cgccccgaat tttaaagggc aattacgata aaaatgtgat tttcatcacg
     4561 atttaggtgc agccatttcg tcgagatcaa gtttaggaca aaattttgcc agttggtgtt
     4621 aatgttttgt tagaatcagt cacgtaatgt gagcacgatt aaagtgagat gtgagcaaat
     4681 ggctaataaa ccttcggcag aagaactgaa aaaaaatttg tccgagatgc agttttacgt
     4741 gacgcagaat catgggacag agccgccatt tacgggtcgt ttactgcata acaagcgtga
     4801 cggcgtatat cactgtttga tctgcgatgc cccgctgttt cattcccaaa ccaagtatga
     4861 ttccggctgt ggttggccca gtttctacga accggtgagt gaagaatcca ttcgttatat
     4921 caaagacttg tcacatggaa tgcagcgcat agaaattcgt tgcggtaact gtgatgccca
     4981 tctggggcat gtcttccctg acgggccgca gccaacgggc gaacgttatt gtgttaactc
     5041 tgcttcttta cgcttcactg atggcgaaaa cggcgaagaa atcaacggtt gaaaaaacga
     5101 ttcagcaact tattccacag gagcggaaaa attatgaatc ttgatgacat tatcaacagc
     5161 atgacgcctg aggtatacca gcgtttgtcg accgccgttg aactggggaa atggcccgat
     5221 ggcgttgcgt tgaccgagga acaaaaagcg aactgcctgc aactggtgat gctgtggcaa
     5281 gctcgccata ataccgaagc acagcatatg acaattgaca caaatggtca gatggtcatg
     5341 aagagcaaac agcagttaaa agaagatttt ggtatctcag caaagcctat tgctatgttt
     5401 aagtgaatga gttatctagt atttaagcct gttattaagg gagggcaaat atacttgtta
     5461 ggacgtcgtg ttttagctgc aagtaggtta aatgcaacac tgacaagtat tatctggcct
     5521 acaatgtcat tgaggaaatg taggccagag atgaattcaa ctaatattac ccctgtgtct
     5581 cttcccagtc tgccagcgta tatagcgttg ccccagctgc tgacatctcc ataaacgcgt
     5641 gcgcactgtc ctggggctgg atattcacgc cacgacaacc atcggtaatc acatttacct
     5701 tataacctaa ctgtaacgcg tccagcacgg taaacttcac gcaatagtca gtagccaggc
     5761 ccataacgat caattcatcg atttcatgat cgcgtaacca gtcatcgagc gaggttttct
     5821 gccgacggcc gttatcaaaa aaggcactgt aactgtcaac taaaggattt tcgcctttat
     5881 ggaacaccgc tgcgatcgct ttttggttca gtaacggatg taattgtgcg ccttcactgt
     5941 tctgcacaca gtgatctggc cagaaggttt gtggcaaacc gtcgagttgg cctggcgtat
     6001 aaggctctac accgtgctga ctggcaaaac tgccgtgatt cgccgggtgc cagtcctgac
     6061 tggcgataac cgcttcaccg cgcgactggc accagtcaat caggcggtta gcgacatcca
     6121 ccgtactgtc accttccggc acggcgagcg cgccaccagc acagaaatca ttttgtaaat
     6181 cgaccagtaa cagggcgcga gggggcatta cagtctcctt aatcatccgg cgtcagttcg
     6241 ccgcgcaggt tttggctcat ggccttgcga atggtttcag tatccagttc ctggctcagc
     6301 aggtaatgca gtttggttag cgtggcttct acagtcatat ctgcaccgcc aataacgccg
     6361 gcatgggcga gggcgttacc ggtggcataa ccacccatat tcactttacc ggacatacat
     6421 tgtgtcaggt tgaccaccac aataccgcga tcgctggctt cttgtaattc ctgcaggaag
     6481 gctttgtttt gtggcgcatt acccacgcca taggagcgca gaatcaatgc tttcaccggt
     6541 tggcgcagaa aattgcgcac gacgtcagca gaaatccctg gataaatcgt cactacgcca
     6601 attggttgtg gcgtgattgg atgaacgatt aattcccctt caccgtgcgg ggcgggtggc
     6661 gtattcaaac gacgaatatg gataccagct tccagtaacg gaggaaggtt tggagaggca
     6721 aacgcatcaa aaccatcggc atgggctttg gtagtgcggt tgccgcgata caatcggtta
     6781 ttgaaaaaga gcgttacttc gttgatcgga taattcgccg caacgtacaa cgcattcagc
     6841 agattaattt gtccgtcaga gcgtaactca gccagcggga tttgtgaccc tgtcacaatg
     6901 accggtttac cgagattctc gagcatgaac gacagcgcag aggcagtata cgccatcgtg
     6961 tcggtgccgt gcaggatgac aaaaccatca tagtcgtcat agtgcgcttt aatatcttca
     7021 gcaatatgct gccagtcttc tggcgtcata tcagaagaat ccatcagcgg cgtatattca
     7081 tgaatggtga aatctggcat ctccgggcga tggaattccg gcatcagcgc cagttggcgt
     7141 tgtagatgac ctgacaccgg aatataaccc tgctcggaac gctgcatccc gatggtcccg
     7201 cccgtgtagg cgacgtaaat tgatttcttt tgcatgatat tgatatgaat gatatcgaaa
     7261 gagcaaaagt atatacgtga ggcaggagga gcgcacctga tactatgcgg ataaggaaat
     7321 atggggcgag acagcgggaa gaggattcgt cgacgtaaaa aataatgtgg ccgacgatct
     7381 gtcggccact cagtacaaga cttaacgcac gttggcgcag gtcaggcaaa acgcataacg
     7441 gttttgtggg tcgttaaacg cggccagctt gtcgctttca cttttaacgg tagaggctac
     7501 cgaggcaagc ggtgcaggta acatggcctg gaacgcatct ggcaacattg cccggacaga
     7561 accagacatg ttgtccatca ctttgtcgaa gaaggtcggt tcatcaacgt agtattccag
     7621 atgccactgt ttcacttttg ccagctctgc tgctttggcg accgcatcat cgaaatcccc
     7681 gagactatcg accagcccgt tagcttttgc atcctgaccg gtccagacgt ggccctgggc
     7741 gattttatca atctgctccg gcgtcgaatg acgcgcatca gcaaccagcg tgataaagcg
     7801 tttatagcca ttctcaatgc ttaattgcat catctgctgc gcttccggcg gcagtgccct
     7861 ggtgatagaa acatccgcca gcggtgaagt tgagacacca tcagtatgaa caccaatcga
     7921 atccagacta ttttctacgg tggtgatcac gccgaagata ccgatagaac cggtcagggt
     7981 gctggggtta gccacaatgt aattagctgg cgtggaaatc cagtaaccac cagatgccgc
     8041 catgccgccc atcgatacaa ccacaggctt acccgctgcc cgggctgctg ccagttcagc
     8101 gcgaatcact tcagacgcgg taacgctgcc gcctgggcta ttaacacgca ggacaatcgc
     8161 tttcactttc gggtcaaggc gagcgtcgcg gatttgtgcc gcagtggtat caccgccaac
     8221 attcccctga gtttcctcgc catccataat tgcgccatta gcgaagacga caccgatgct
     8281 gtcaccggta tctgccggcg ttttcaatgc gtaatcgtaa taactgatgg cgcgataatt
     8341 tttatcagtc ttactccagc cgaactcttt ggttagagct ttttcgattt cggcactcga
     8401 tgccagtgca tcgaccagct tgttttccag tgcatatttc gcggtatcgc caccggtttt
     8461 ggttaaaccc tcaagcaacc cttgcgcgcc agggaatacc tgctgagcag ggatctgccg
     8521 gttagcggca acagtattca gatagttttg ccacagctca ccaatccagc ggctgtcagc
     8581 ttcgcgggct gccggtgaca tgtcatcacg aataaacggt tcaacggcag atttatacgt
     8641 acccacgcgg aacacatggg tggaaacttt cagcttatcc agcaacgatt tgtagtacag
     8701 accgttggta gcaaaaccgt gcaaatccac cacgccttgc ggtgacagcc aaattttatt
     8761 ggcgaaactg gcgagataat attgcccctg gctgtagttc tcgccaacgg cataaaccgg
     8821 tttcccgctg tcacgaaact ctttcagggc tttgccgatg tactgcatag acggctggtc
     8881 gccgcctgcg aagtttttca gatccatcac gataccggtg atattgcggt cgtccttcgc
     8941 ctggcgaatg gtgttgacga tatcaaacag tgagttttcc tgcagacgat cggaactggc
     9001 accaagcagc tggcggctta atttactaaa ccgctgagaa ctgtcgggtt tatcgacgat
     9061 cacaccagaa atgtccagca gcagtgcgcc acgactggcc gtttctttcg aatcaccacc
     9121 actgacctgc atccagatcc ccacaccaac cagtacgagg aaaataaaga acaggttaag
     9181 taccatttca cggacgaaat tcagcagacg ccacgtccat ttaaaaaatc cggcaataaa
     9241 tcgccaaagg gttcgcatgt attctcccaa cttaaggtca cacctgtcac aggcgcaata
     9301 gatgtggtta tcgtaatgac ccaaccggca cttgtcagca ggaattgcgg actgtgctgt
     9361 aacaaaatcc actctcgtgt taattttgtg aataaatatc acgacaggag ttaatcaaat
     9421 ggatgcactc gaactattga tcaatcgccg tagcgcctcc cgcctggctg aacccgcgcc
     9481 aacgggtgaa caactgcaaa acatcctgcg tgcgggtatg cgtgcgccgg accataagtc
     9541 catgcaaccg tggcattttt ttgtgattga aggggaaggg cgcgagcgtt tcagcgccgt
     9601 actggaacag ggggcgattg ctgccggtag tgatgacaaa gctatcgaca aagcccgtaa
     9661 tgcgccgttc cgcgcaccgc tcatcatcac ggtggtggcg aaatgcgaag aaaatcataa
     9721 agtcccgcga tgggaacagg aaatgtctgc cggatgcgcg gtcatggcga tgcaaatggc
     9781 agccgttgcc cagggatttg gcggcatctg gcgcagtggc gcattaactg aaagtccggt
     9841 agtgcgtgaa gcattcggtt gccgtgagca ggataaaatt gtcggttttc tctacctcgg
     9901 cacgccgcag ttgaaagcat ctacgtcgat taacgtcccg gacccgacgc cgtttgtaac
     9961 ttatttctga taattgtcgc gaaactgtct ggattgtgag caaaggcgca ggaattcaga
    10021 cactctcact tatcacttca cggaatgagg gttaccatac ccggattgca ttgacaggag
    10081 atgtccatga gcgagaactc gattcgtttg acccaataca gccacggagc tggttgcggc
    10141 tgtaaaattt ccccaaaagt gttggaaacc atcctgcaca gtgagcaggc gaagtttgtt
    10201 gatccgaatt tgcttgtggg taatgagacc cgcgacgatg cggcggtgta cgatctgggc
    10261 aatggcacca gcgttatcag taccaccgac ttctttatgc cgatcgttga taatcctttc
    10321 gattttggcc gcattgcggc gactaacgcc atcagcgata tcttcgcgat ggggggcaaa
    10381 ccgattatgg cgattgcgat cctcggctgg ccgattaaca aactttcccc ggaaattgcc
    10441 cgcgaagtaa ccgaaggtgg acgctatgca tgccgtcagg cgggtattgc gctggctggc
    10501 ggtcactcca tcgatgcgcc ggagccgatt tttggtctgg cggtaacggg gatcgtaccg
    10561 accgagcggg tgaagaaaaa cagtaccgca caagccggat gcaaactgtt cctgacgaaa
    10621 ccgctgggga tcggcgttct taccacggct gagaaaaaat cactgctgaa accagaacat
    10681 cagggactag cgacggaagt gatgtgccgg atgaacatcg caggcgcgtc ctttgccaac
    10741 atcgaaggcg taaaagcgat gaccgacgtt acgggttttg gtctgctggg ccatttgagc
    10801 gaaatgtgtc agggcgcagg tgtgcaggcg cgcgttgact atgacgcgat cccaaaattg
    10861 ccgggggttg aagagtacat taagttgggc gcagtgcctg gcggcaccga acgtaacttt
    10921 gccagctacg gtcatctgat gggtgaaatg ccgcgtgaag tgcgcgattt gctgtgcgat
    10981 ccgcaaactt ctggcggttt gctgctggcg gtcatgccgg aagcagaaaa tgaggtcaaa
    11041 gctacagccg ccgagtttgg cattgaactg acggcaattg gcgaactggt gccagcgcgc
    11101 ggcggtcgtg ccatggttga gattcgttaa ttcaatgcgg ttgtttattg ccgaaaaacc
    11161 gagtctggcg cgcgccattg ctgatgtcct gcccaaaccg caccggaaag gcgatggctt
    11221 tatcgagtgc ggtaatggtc aggtggtgac ctggtgtatc ggtcacctgc ttgagcaggc
    11281 gcagccagac gcctacgaca gccgctatgc gcgctggaat cttgcggatt tgccgattgt
    11341 cccggaaaag tggcaattac agccccgacc ctccgtgacc aaacaactta acgtcatcaa
    11401 acgcttcctg catgaagcca gcgaaatcgt tcacgccggg gacccggatc gtgaagggca
    11461 actgctggtg gatgaagtgc tggactatct gcaactggca ccggaaaagc gccagcaggt
    11521 gcagcgttgc ttgataaacg acctgaaccc gcaggcggtt gagcgggcgc tcgaccgtct
    11581 tcggtccaac agtgagtttg tgccattgtg cgtttctgcg ctggcgcgag cgcgtgccga
    11641 ctggctgtac ggcatcaata tgacccgtgc gtataccatt ctcggtcgca atgccggtta
    11701 tcagggcgta ctttccgtgg gacgcgtgca gacacccgtg ctcgggctgg tggtgcgccg
    11761 cgatgaagag attgaaaact tcgtggcgaa agacttcttt gaagtcaaag cacatatcgt
    11821 gacacctgcc gatgagcggt ttaccgctat ctggcaaccg agcgaagcgt gtgaaccgta
    11881 ccaggatgaa gaagggcgct tgttacatcg tccactggcg gagcatgtgg ttaaccgcat
    11941 tagtggtcaa ccagcaattg tcaccagcta taacgataaa cgggaatcag aatccgcgcc
    12001 gctgcctttt tcgctttcgg cgttgcagat tgaagcggca aaacgctttg gtctgagcgc
    12061 gcagaacgtg cttgatatct gccagaagct gtacgaaacg cacaagctaa tcacttatcc
    12121 gcgttccgat tgtcgctatt tgccagaaga acattttgcc ggacgccacg cggtgatgaa
    12181 tgccatcagc gttcatgcac cagatctgtt gctgcagcca gtggtagatc cagatatacg
    12241 caaccgctgt tgggatgaca aaaaggtcga tgcacaccac gccatcatcc cgaccgcacg
    12301 gagttcagcc atcaacctga cggagaacga agcgaaggtc tataacctga ttgcccgtca
    12361 gtacttgatg cagttctgcc cggatgtggt gttccgcaag tgtgttatcg aactggacat
    12421 tgccaaaggc aaatttgtcg ctaaagcgcg ttttcttgct gaagcaggct ggcgcacgct
    12481 gttaggcagc aaagagcgcg atgaagaaaa cgacggtacg ccattgcctg tggtggcgaa
    12541 aggcgatgag ttgctgtgtg aaaaaggtga agtggtagag cggcaaaccc agccgccgcg
    12601 ccattttacc gatgcaacac tgctttcggc gatgaccggg atcgcgcgct ttgtgcagga
    12661 caaagatctg aaaaagatcc ttcgtgcgac cgatggtctg gggacagaag caacccgtgc
    12721 cgggattatt gaactgctgt tcaagcgtgg ttttctgacc aaaaaagggc gctatatcca
    12781 ctctaccgac gccggaaaag cgttattcca ttcgctgcca gaaatggcga cgcgaccgga
    12841 catgaccgcg cactgggaat cggtgctgac gcaaatcagc gaaaagcagt gtcgctatca
    12901 ggactttatg cagccgctgg tggggacgct atatcagctt attgatcaag ccaaacgtac
    12961 gccggtgcgg cagtttcgcg gcattgtggc tccgggcagt ggtggcagtg ctgataagaa
    13021 aaaggctgca ccgcgtaaac gtagtgcgaa aaaaagtccg ccagcagatg aagtcggaag
    13081 cggggcgata gcgtaagcga gtgaatcttt cgtgctattc gag
//
LOCUS       DAZPLI010000114        13047 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZPLI010000114 DAZPLI010000000
VERSION     DAZPLI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13047
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.115"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            75..374
                     /locus_tag="ACHUU3_003057"
     CDS             75..374
                     /locus_tag="ACHUU3_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083068.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(421..1308)
                     /gene="rarD"
                     /locus_tag="ACHUU3_003058"
     CDS             complement(421..1308)
                     /gene="rarD"
                     /locus_tag="ACHUU3_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR2083069.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1360..1827)
                     /gene="yigI"
                     /locus_tag="ACHUU3_003059"
     CDS             complement(1360..1827)
                     /gene="yigI"
                     /locus_tag="ACHUU3_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR2083070.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1992..2861
                     /gene="pldA"
                     /locus_tag="ACHUU3_003060"
     CDS             1992..2861
                     /gene="pldA"
                     /locus_tag="ACHUU3_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR2083071.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2994..4823
                     /gene="recQ"
                     /locus_tag="ACHUU3_003061"
     CDS             2994..4823
                     /gene="recQ"
                     /locus_tag="ACHUU3_003061"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR2083072.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4887..5504
                     /gene="rhtC"
                     /locus_tag="ACHUU3_003062"
     CDS             4887..5504
                     /gene="rhtC"
                     /locus_tag="ACHUU3_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR2083073.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5566..6186)
                     /gene="rhtB"
                     /locus_tag="ACHUU3_003063"
     CDS             complement(5566..6186)
                     /gene="rhtB"
                     /locus_tag="ACHUU3_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR2083074.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6297..7319
                     /gene="pldB"
                     /locus_tag="ACHUU3_003064"
     CDS             6297..7319
                     /gene="pldB"
                     /locus_tag="ACHUU3_003064"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR2083075.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7327..8127
                     /gene="yigL"
                     /locus_tag="ACHUU3_003065"
     CDS             7327..8127
                     /gene="yigL"
                     /locus_tag="ACHUU3_003065"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR2083076.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPYYLRKLYLS"
     gene            8203..9102
                     /gene="bioP"
                     /locus_tag="ACHUU3_003066"
     CDS             8203..9102
                     /gene="bioP"
                     /locus_tag="ACHUU3_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR2083077.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8990..9943)
                     /gene="metR"
                     /locus_tag="ACHUU3_003067"
     CDS             complement(8990..9943)
                     /gene="metR"
                     /locus_tag="ACHUU3_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR2083078.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10179..12440
                     /gene="metE"
                     /locus_tag="ACHUU3_003068"
     CDS             10179..12440
                     /gene="metE"
                     /locus_tag="ACHUU3_003068"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR2083079.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12562..12684)
                     /locus_tag="ACHUU3_003069"
     CDS             complement(12562..12684)
                     /locus_tag="ACHUU3_003069"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083080.1"
                     /translation="MRKNDSVISVSERAQIAKWLPVMEAIRLLAKCALFMPDAA"
     gene            complement(12725..12970)
                     /locus_tag="ACHUU3_003070"
     CDS             complement(12725..12970)
                     /locus_tag="ACHUU3_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR2083081.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3085 a         3250 c         3479 g         3233 t
ORIGIN      
        1 gggtatattt taattaaata taattgaaat gtatttttat tctataaaca atatcttggg
       61 tgaatggatt ctatatggga aatggtaact tttggcagtt gttcttgggt aaagaacctt
      121 acagcaaaaa atctgatttt gttaaggcat ggttattgtg gtgggctatt accatccttg
      181 ttctatttat tttaagcctg actacgacat taaatttaag aagtataggt atagggttaa
      241 gttttgggtt ttgtcaaatc cttggtagat atacgatgtc tctaggatgg agtaagtact
      301 ggggagtttt gggggtattt cctgtaacaa atattgttct ttttcttatc ttaatttttt
      361 tcaaaaaaag ataaataagt cagtgggtag gctctttaaa cggcctaccc atgttaattc
      421 ttaacttttt ctacgctgcg tataaatcgc atccatcaca aaaattgcca gcgccaccca
      481 aataaaggcg aaagtcacca tcttatcggc acccggtttt tcaccataaa acgtcacagc
      541 cagcaggaac atcagcgtcg ggccaatgta ctggaaaaag cctaacgttg agagacgcaa
      601 gcgcgtggca gcggcggtaa aacacaacag cggtacggta gtgacaatac cggcggcgat
      661 cagcagtaaa ttcagcgaca tcgggttttg ccccatatgg ctggttgagc tgtcggcaat
      721 agcaaacagg taaattgccg ccacgggcag cagccacatg gtttcgatta acatgccggt
      781 ttgcgcttca acggcaatct tcttgcgtac cagaccgtag aaggcaaaac taaatgccag
      841 tcccagcgcg ataataggta gcgaaccaaa agtccacagc tggactaaca cgccacatat
      901 cgccagaatc accgccagcc attgcatccg gcggaatcgc tcgccgagga aaatcatccc
      961 cagcacaatg ttcaccagcg ggttaataaa gtaaccaagg ctcgcttcca gcatatggtg
     1021 attgttcacc gcccagataa acagtagcca gttgccacca atcagcacgg cagagactgc
     1081 cagcataaaa attttctgtg gcgtctgaat cagcgttttt aaataggacc actggcggca
     1141 aatgctcatc agcaccacca taaagaaaaa cgaccagatc acgcgatgcg tcaggatttc
     1201 atcggcgggc acgtagtaaa tcaacttgaa gtacgctggc gctatacccc aaataaaata
     1261 agcggcaaga gcgagtaata cgccctgccg cgtttgtttt gcatccatcg ggaatactca
     1321 tttttaattg gtaacagcag tttacctgct tttatgtctt caacctacca tataggtggc
     1381 ggtggcactg gcaatataaa gctgttcttc attgtgtaat tcaacgcggg cgacggcgac
     1441 tttattgcct gcacgcaaca ggctactggt agcagtaaaa cgctcgcccc tgcctgggcg
     1501 cagataatca acgcgaagat caatggtccc catccgcgat agccgctggc gtagttcatc
     1561 ttcactgatg gtttcgtggc gggttaaggt acttcccacg cacaccagac cggcggcgac
     1621 atccagcgcc gacgcaatga ccccgccgtg caaaatgctt tgcgcccagt tgcccaccat
     1681 cattggctga tttttaaagg ccagctgtgc gaactctttt tcgtaacgct ccagttccat
     1741 ccccaatgcg cggttaaatg gcatgtgata aacaaacatc tcacccacta atttcagggc
     1801 ttgttcagcg gtcagtacgg cagacatatc atccttacac ttcattggtt aatgaaatgt
     1861 tgattttatg cttctttgtt gttggtttct actttaggaa gggataacta acggctatgg
     1921 agttaagtat gtaaaatagc ccgcagaaaa atattcacct tatcaataat tcgttacgga
     1981 gaacacgacc gatgcggact ctgcagggct ggttgttgcc ggtgtttatg ttgcctatgg
     2041 cagtatatgc acaagaggca acggtgaaag aggtgcatga cgcgccagcg gtgcgtggca
     2101 gtattatcgc caatatgctg caggagcatg acaatccgtt cacgctctat ccttatgaca
     2161 ccaactacct catttacacc caaaccagcg atctgaataa agaagcgatt gccagttacg
     2221 actgggcgga aaatgcgcgt aaggatgaag taaagtttca gttgagcctg gcatttccgc
     2281 tgtggcgtgg gattttaggc ccgaactcgg tgttgggtgc gtcttatacg caaaaatcct
     2341 ggtggcaact gtccaatagc gaagagtctt caccgtttcg tgaaaccaac tacgaaccgc
     2401 aattgttcct cggttttgcc accgattact gttttgcagg ttggacgctg cgcgatgtgg
     2461 agatggggta taaccacgac tctaacgggc gttccgaccc gacctcccgc agctggaacc
     2521 gcctttatac tcgcctgatg gcagaaaacg gtaactggct ggtagaagtg aagccgtggt
     2581 atgtggtggg taatactgac gataacccgg atatcaccaa atatatgggt tactaccagc
     2641 ttaaaatcgg ctatcacctc ggtgatgcgg tgttgagtgc gaaaggacag tacaactgga
     2701 ataccggtta cggcggcgcg gagttaggct taagttaccc gatcaccaaa catgtgcgcc
     2761 tttatactca ggtttacagc ggctatggcg aatcgctcat cgactataac ttcaaccaga
     2821 cccgtgtcgg tgtaggagtt atgctaaacg acttgttttg atgaacggtt gagtggttgg
     2881 caaatctgga acccagcatc caggattacc ctctcagaga ctaaaagcat tgcagtttct
     2941 cgcgcaggcg ctgaaaatag cgcctgtttt tatttcaggc aatcggggtg aatgtggcgc
     3001 aggcggaagt gttgaatctg gagtccggag ctaaacaggt tttacaagaa acctttggct
     3061 accaacagtt tcgccccggc caggaagaaa ttatcgacac tgtgctttcc ggtcgcgatt
     3121 gcctggtcgt catgcccacc ggtggcggaa aatccctttg ctatcaaatt cctgccttat
     3181 tgctaaacgg ccttaccgtg gttgtttcac cgctgatttc gttgatgaaa gatcaggtgg
     3241 atcaactgca agccaacggc gtggcggcgg cgtgccttaa ctcgacgcaa acccgcgagc
     3301 agcaacttga agtgatgaca ggctgccgca ccgggcaaat tcgtctgctt tatatcgccc
     3361 cggaacgcct gatgctggat aactttcttg agcatctggc gcactggaat ccggtgttat
     3421 tagccgttga tgaagcgcac tgtatctccc aatggggcca cgatttccgc ccggaatatg
     3481 ccgcgcttgg tcagttacgc cagcgtttcc cgaccctgcc gtttatggcg ctgactgcca
     3541 cagccgacga caccacgcgc caggatatcg tgcgcttgct ggggctgaac gatccgctga
     3601 ttcaaatcag cagttttgac cgtccgaata ttcgctacat gctgatggag aaattcaaac
     3661 cgctcgatca gttgatgcgc tacgtgcagg aacagcgcgg taagtccggc attatctact
     3721 gcaacagccg ggcgaaagta gaagacaccg ctgcgcgcct gcaaagcaag ggtattagcg
     3781 cggcggccta tcatgccggg ctggaaaata atgtccgcgc cgacgtgcag gagaaattcc
     3841 agcgcgatga cctgcaaatt gtggtggcga cggtggcgtt cggcatgggc atcaataaac
     3901 caaacgttcg cttcgtggtc cactttgata ttccgcgcaa tatcgaatcc tattatcagg
     3961 aaaccggtcg cgccgggcgt gatggcctgc ctgcggaagc gatgctgttt tacgatccgg
     4021 ctgatatggc gtggctgcgc cgttgtctgg aagagaagcc gcaggggcag ttgcaggata
     4081 ttgagcgcca caaactcaat gcgatgggcg cgtttgccga agcgcaaact tgccgtcgtc
     4141 tggtattgct gaactatttt ggcgaagggc gtcaggagcc gtgcgggaac tgcgatatct
     4201 gcctcgatcc gccgaaacag tacgacggtt caaccgatgc tcagattgcc ctttccacca
     4261 ttggtcgtgt gaatcagcgg tttgggatgg gttatgtggt ggaagtgatt cgtggtgcta
     4321 ataaccagcg tatccgcgac tatggtcatg acaaactgaa agtctatggc atgggccgtg
     4381 ataaaagcca tgaacattgg gtgagcgtga tccgccagct gattcacctc ggcctggtga
     4441 cgcaaaatat tgcccagcat tctgccctac aactgacaga ggccgcgcgc ccggtgctgc
     4501 gcggcgaatc ctctttgcaa cttgccgtgc cgcgtatcgt ggcgctcaaa ccgaaagcga
     4561 tgcagaaatc gttcggcggc aactatgatc gcaaactgtt cgccaaatta cgcaaactgc
     4621 gtaaatcgat tgccgatgaa agtaatgtcc cgccgtacgt ggtgtttaac gacgcaacct
     4681 tgattgagat ggctgaacag atgccgatca ccgccagcga aatgctcagc gttaacggcg
     4741 ttgggatgcg caagctggaa cgctttggta aaccgtttat ggcgctgatt cgtgcgcatg
     4801 tcgatggcga cgacgaagag tagtcagcag cataaaaaag tgccaggatg aagactccgt
     4861 aaacgtttcc cccgcgagtc aaatgtatgt tgatgttatt tctcaccgtc gccatggtgc
     4921 acattgtggc gcttatgagc cccggtcccg atttcttttt tgtctctcag accgctgtca
     4981 gtcgttcccg taaagaagcg atgatgggcg tgctgggcat tacctgcggt gtaatggttt
     5041 gggctgggat tgcgttgctt ggcctgcatt tgattatcga aaaaatggcc tggctgcata
     5101 cgcttatggt tggcggtggc ctgtatctct gctggatggg ttaccagatg ctgcgcggcg
     5161 cgctgaaaaa agaggcggtt tctgcacctg cgccacaggt cgagctggcg aaaagtgggc
     5221 gcagtttcct gaaaggttta ctgaccaatc tcgctaatcc gaaagcgatt atctactttg
     5281 gctcggtgtt ctcattgttt gtcggtgata acgttggcac taccgcgcgc tggggcattt
     5341 ttgcgctgat cattgtcgaa acgctggcgt ggtttatcgt cgttgccagc ctgtttgccc
     5401 tgccgcaaat gcgccgtggt tatcaacgtc tggcgaagtg gattgatggt tttgccgggg
     5461 cgttatttgc cggatttggc attcatttga ttatttcgcg gtgatgccag acgcgtcttc
     5521 agagtaagtc ggataaggcg tttacgccgc atccgacatt atttttcacg catgcctcgc
     5581 cgatgctaac agcgctccca ccagcataaa caacgagccg aaaatcttat tcagcgcctt
     5641 catctgcttt ggtcctttaa tccatagagc aatccgttga gcaagggtgg cgtaaccgat
     5701 catcacaata atatcgacca caatagtggt gacgccgagc acgatatact gcatcagttg
     5761 cggctgttgc ggcatgatga attgcggaaa tagcgccgcc agaaacacaa tacttttggg
     5821 attggtgaga ttcacaaaaa ctgcgcgctg gaacaaatgt cgacgcgatt gagtagaggc
     5881 cagcgattta aggtcaattg caccagcggc gcgccactgc tggattccca gccaaatcaa
     5941 gtaagccgcg cctgcccact tcaacacttc aaacgcaatc actgagcggg aaaatagcgt
     6001 ccccaacccc acgccaacca gcacaatatg aatcgccagt ccggtctgaa gcccagcaat
     6061 agacgccacc gcgccgcgat aaccgtggtt gagcgaggtg gtcatagtgt tgattgcacc
     6121 agagcctggc gacaggctta aaatgatcga tgtcagcagg taggcaaacc accattctaa
     6181 ggtcatgatg aactcccggt gtgtctattt ttgtgccaca atacgctact gtcgcagcgt
     6241 tgtgtcaggc acgctaaaaa aaacgatttt acgtggttta agaggcagat tacccgatgt
     6301 ttcagcagca aaaagactgg gaaacaagag aaaacgcgtt tgctgctttt accatgggac
     6361 cgctgactga tttctggcgt cagcgtgatg aagcagagtt tactggtgtg gatgacattc
     6421 cggtgcgctt tgtccgtttt cgcgcacagc accatgaccg ggtggtagtc atctgcccgg
     6481 ggcgtattga gagctacgta aaatatgcgg aactggccta tgacctgttc catttggggt
     6541 ttgatgtctt aatcatcgac catcgcgggc agggacgttc cggtcgcctg ttagccgatc
     6601 cgcatctcgg gcatgttaat cgctttaatg attatgttga tgatctggcg gcattctggc
     6661 agcaggaggt tcagcccggt ccgtggcgta aacgctatat actggcacat tcgatgggcg
     6721 gtgcgatctc cacattattt ctgcaacgcc atccaggtgt atgtgacgcc attgcgctaa
     6781 ctgcgccaat gtttgggatc gtgattcgta tgccgtcatt tatggcacgg cagatcctca
     6841 actgggccga agcgcatcca cgtttccgtg atggctatgc aataggcacc gggcgctggc
     6901 gcgcgttgcc gtttgctatc aacgtactga cccacagcag acagcgatat cgacgtaact
     6961 tacgcttcta tgctgatgac ccaacgattc gcgtcggtgg gccgacctac cattgggtac
     7021 gcgaaagtat tctggctggc gaacaggtgt tagccggtgc gggtgatgac gccacgccaa
     7081 cgcttctctt gcaggctgaa gaggaacgcg tggtggataa ccgcatgcat gaccgttttt
     7141 gtgaactccg caccgccgcg ggccatcctg tcgaaggagg acgaccgttg gtaattaaag
     7201 gtgcttacca tgagatcctt tttgaaaagg acgcaatgcg ctcagtcgcg ctccacgcca
     7261 ttgttgattt tttcaacagg cataactcac ccagcggaaa ccgctctaca gaggtttaaa
     7321 tttcttatgt accaggttgt tgcgtctgat ttagatggca cgttactttc tcccgaccat
     7381 acgttatccc cttacgccaa agaaaccctg aagctgctca ccgcgcgcgg catcaacttt
     7441 gtgtttgcga ccggtcgtca ccacgttgat gtggggcaaa ttcgcgataa tctggagatc
     7501 aagtcgtaca tgatcacctc caacggcgcg cgcgttcacg atctggatgg caatctgatt
     7561 tttgctcata acctggatcg cgacattgcc agcgatctgt ttggcgtagt caacgacaat
     7621 ctggacatca ttactaacgt ttatcgcgac gacgaatggt ttatgaatcg ccatcgcccg
     7681 gaagagatgc gcttttttaa agaagcggtg ttcaaatatg cgctgtatga gcctggatta
     7741 ctggagccgg aaggcgtcag caaagtgttc ttcacctgcg attcccatga acaactgctg
     7801 ccgctggagc aggcgattaa cgctcgttgg ggcgatcgcg tcaacgttag tttctctacc
     7861 ttaacctgtc tggaagtgat ggcgggcggc gtttcaaaag gccatgcgct ggaagcggtg
     7921 gcgaagaaac tgggctacag cctgaaggat tgtattgcgt ttggtgacgg gatgaacgat
     7981 gccgaaatgc tgtcgatggc ggggaaaggc tgcattatgg gcagtgcgca ccagcgtctg
     8041 aaagaccttc atcccgagct ggaagtgatt ggtactaatg ccgacgacgc ggtgccgtat
     8101 tatctgcgta aactctattt atcgtaatcg ttctttattt ggtcagttgt caacctgata
     8161 cttcgctaca atggatactc gttaatcaaa gagttttcca ttgtggcgct acttatcatc
     8221 accacgattc tgtgggcctt ctcctttagc ttttatggcg agtaccttgc ggggcacgtc
     8281 gatagctatt ttgcggtgct ggtgcgcgtt ggcctggcgg cactcgtttt tctgccgttt
     8341 ctgcgtaccc gtggcaatag cctgaaaacg gtcggcctgt atatgctggt gggcgcgatg
     8401 cagctcggcg tgatgtatat gctgagtttc cgcgcttatc tctacctgac ggtctccgaa
     8461 ttgttgctgt tcaccgtact gacgccgctc tacatcacgc tgatttatga catcatgagt
     8521 aagcgccgtc tgcgctgggg ctatgctttt agcgccttgc tggcggtgat tggtgccggg
     8581 attattcgct atgatcaggt taccgaccat ttctggactg gcttgttgct ggtgcaactc
     8641 tccaatatca cttttgccat cggcatggtg ggttacaaac gcctgatgga aactcgcccg
     8701 atgccgcagc ataacgcctt tgcgtggttc tatcttggcg cgttcctggt ggcggtaatt
     8761 gcatggttct tgctgggaaa tgcgcagaaa atgccgcaaa ccacgttgca atggggcatt
     8821 ctggtgtttc ttggcgtggt ggcttccggg attggctact ttatgtggaa ctacggcgcg
     8881 acgcaggtgg acgccggaac gctgggcatt atgaataata tgcacgttcc ggcagggctg
     8941 ctggtaaacc tggctatctg gcaccaacag ccgcactggc caacgttcat tacaggcgcg
     9001 ctggtgatcc tggcctcact gtgggtgcat cgtaagtggg tcgctccgcg ctcttcacaa
     9061 acggcagatg atcgcaggcg tgattgcgcg ctgagcgaat aaatgcttct gtaatcggct
     9121 gacgctgctc gccatcgcgc acggcggcgt acagtcggct ccacaagcct tcgcccaggg
     9181 ttttggtcac caccagaccc tggcgctcaa aactctctac tacccaatgc ggtagcgcgg
     9241 caatacccat ccgcgcggca accatctgaa tcaacaataa ggtgttatcg acgcttttca
     9301 gtgacgggct gacgcctgcc ggctgaagaa aatgccgcca gacatccagt cgactacgct
     9361 gcaccggata aatcaacagc gtctcgctgg cgagatcttc cggtgtaatt cgcgttttcg
     9421 ccgccagtgg atggtcaggt gctaacacca gacgcacttc atagtcgaac atcggcgaat
     9481 aatgcaggcc actgcgcggc aggatatcgg aggtcatcac cagatccagc tcgccctgtt
     9541 gcaacgcagg ctgcgggtca aacgtcacgc ccgatttaaa atccatctct acctgcggcc
     9601 agttcttatg gaaattttct aacgcgggcg tcagccactg aatacagcta tggcactcaa
     9661 tggcaatgcg cagacgcgtc tgctgcggtt cattgcaggc ttgcagtgcc tggctgattt
     9721 gcggcagcac ctggtttgcc agttgcaaca ggatttctcc ctgcggtgta aagcgtagcg
     9781 gctggctctt acgcacaaat agccggaagc caaggcgttg ttccagatcg ctaaactggt
     9841 gagacagggc ggattgcgtc tgatgcaacg tcgccgcagc ggctgcgaga gagccgcagt
     9901 tccgcaacgc ttgtagcgtt ttcaggtgtt ttacttcgat catgaaagtc cttcacttcg
     9961 gcatgaataa tttgcgcttg aggaatatac agtaaccgcc aattatggat gtgtaaacat
    10021 ctggacggct aaaatccttc gtcttttaaa tttatggtgc gttggctgcg tttctccacc
    10081 ccggtcactt acttcagtaa gctcccgggg atgaataaac ttgccgcctt ccctaaattc
    10141 aaaatccata ggatttacat ataattagag gaagaaaaat gacaatattg aatcacaccc
    10201 tcggtttccc tcgcgttggc ctgcgtcgcg agctgaaaaa agcgcaagaa agttattggg
    10261 cggggaactc cacgcgtgaa gaactgctga cagtagggcg tgaactgcgt gctcgtcact
    10321 gggatcaaca aaagcaagcg ggtatcgacc tgctgccggt gggcgatttt gcctggtacg
    10381 atcatgtact gaccaccagt ttgctgctgg gtaatgttcc gccacgtcat cagaacaaag
    10441 acggttcggt agatatcgac accctgttcc gtattggtcg tggacgtgcg ccgactggcg
    10501 aacctgcggc ggcagcggaa atgaccaaat ggtttaacac caactatcac tacatggtgc
    10561 cggagttcgt taaaggccaa cagttcaaac tgacctggac gcagctgctg gaggaagtgg
    10621 acgaggcgct ggcgctgggc cacaacgtta agcctgtgct gctggggccg gttacttacc
    10681 tgtggctggg gaaagtgaaa ggtgaacagt ttgaccgcct tagcctgctg aacgacattc
    10741 tgccggttta tcagcaagtg ctggcagaac tggcgaaacg cggcatcgag tgggtacaga
    10801 ttgatgaacc cgcgctggta ctggaactac cacaggcgtg gctggacgca tacaaacccg
    10861 cttacgacgc gctccaggga caggtgaaac tgctgctgac cacctatttt gaaggcgtaa
    10921 cgccaaatct cgacacgatt actgcgctgc ctgttcaggg tctgcatgtt gatctcgtac
    10981 atggtaaaga tgacgttgct gaactgcaca agcgcctgcc ttctgactgg ttgctgtctg
    11041 cgggtctgat caatggtcgt aacgtctggc gcgccgatct taccgagaaa tatgcgcaaa
    11101 ttaaggacat tgtcggcaaa cgtgatttgt gggtggcatc ttcctgctcg ttgctgcaca
    11161 gccccatcga cctgagcgtg gaaacgcgtc ttgatgcaga agtgaaaagc tggtttgcct
    11221 tcgccctaca aaaatgccat gaactagcac tgctgcgcga tgcgctgaac agtggtgaca
    11281 cggcagctct ggcagagtgg agcgccccaa ttcaggcgcg tcgtcactct actcgtgtac
    11341 ataatccggc ggtagaaaag cgtctggcgg cgatcaccgc ccaggacagc cagcgtgcga
    11401 atgtctatga agtgcgtgct gaagcccagc gtgcacgttt taaactgcca gcgtggccga
    11461 ccaccacgat tggttccttc ccgcaaacca cggaaattcg taccctgcgt ctggatttca
    11521 aaaagggcaa tctcgacgcc aataactacc gcacgggcat tgcggaacat atcaggcagg
    11581 ccattgttga acaggaacgt ttgggactgg atgtgctggt acatggcgag gccgagcgta
    11641 atgacatggt ggaatacttt ggcgagcacc tcgacgggtt tgtctttacg caaaacggtt
    11701 gggtacagag ctacggttcc cgctgcgtga agccaccgat tgttattggt gacgttagtc
    11761 gcccggcacc gattaccgtg gagtgggcga agtatgcgca atctctgact gataaaccgg
    11821 tgaaagggat gctgactggc ccggtgacta ttctctgctg gtcgttcccg cgtgaagatg
    11881 tcagccgtga aaccatcgcc aaacagattg cgctggcgct gcgtgatgaa gtggccgatc
    11941 tggaagccgc tggaattggc atcatccaga ttgacgaacc ggctctgcgc gaaggtttac
    12001 cgctgcgtcg tagcgactgg gatgcgtatc tccagtgggg cgtagaggcc ttccgtatca
    12061 acgccgccgt ggcgaaagat gacacacaaa tccacactca catgtgttat tgcgagttca
    12121 acgacatcat ggattcgatt gcggcactgg acgcagacgt catcaccatc gaaacctcgc
    12181 gttctgacat ggagttgctg gagtcgttcg aagagtttga ttatccgaat gaaatcggtc
    12241 caggcgtcta tgacattcac tcgccaaacg taccgagcgt ggaatggatt gaagccttgc
    12301 tgaagaaagc ggcaaaacgc attccggcag agcgcctgtg ggttaacccg gactgtggcc
    12361 tgaaaacgcg cggctggcca gaaacccgcg cggcactggc gaacatggtg caggcggcgc
    12421 agaatttgcg tcggggatga tgaatattaa cgagtcactg taaattgcag tacgatgaaa
    12481 gaggcaaaac tgcctgatac taggcctaca taatttatgc aatttattga atttttgggt
    12541 tttgttggcc ggacaaggcg ttcacgccgc atccggcatg aacaacgcgc actttgccaa
    12601 taatctgatg gcttccatca ccggaagcca ttttgctatc tgcgcccgtt cagatacaga
    12661 aataaccgaa tcatttttac gcaaaatata atcctgagcg atttgttcag taatcaacgc
    12721 tacgttattt attctcactt ttcacgaaat tgtatccctt ttcagtaacg tggtagtgag
    12781 gcatagactt atgtccgcta tcctcaataa taaccaaacc ctcgttagct aatttgttaa
    12841 caatgttaac aacattaccg aatcctggaa tttgccttat ggatagtgtg cgatcgagat
    12901 tcatccagtt ccagtttata tctttctcag ccataatttt cagtacgtta atttcagttt
    12961 cagttatcat agtcaagtgc atcctggtgg tataagtcat taagttctcc cttaagttta
    13021 taatcttcta gagtagcagt atgcgta
//
LOCUS       DAZPLI010000115        12996 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZPLI010000115 DAZPLI010000000
VERSION     DAZPLI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.116"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..189
                     /locus_tag="ACHUU3_003071"
     CDS             <1..189
                     /locus_tag="ACHUU3_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083082.1"
                     /translation="FHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQ
                     AGCPTVYPLALASTSVKN"
     gene            189..1391
                     /locus_tag="ACHUU3_003072"
     CDS             189..1391
                     /locus_tag="ACHUU3_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFR2083083.1"
                     /translation="MNLSANAQATLLLTSDFSRAAASEYKPLSNSEWGKFALWLKHQR
                     ISPAELLVPQPQEKLTGWSDPRISQERILGLLARGHSLVLAVDKWQRAGLWILTRGDA
                     DYPVRLKNRLRTDAPPVLFGCGNKALLQAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQ
                     GICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLIAGNLVLISP
                     FYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQ
                     DMQSANSRLVENGASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYA
                     VMPAYTPVDFYQLFVAELAILAKESISIERLASCTGLTIEQISVWLNRAEEEGRVIRL
                     GEGHYQFR"
     gene            complement(1437..2030)
                     /gene="yhgN"
                     /locus_tag="ACHUU3_003073"
     CDS             complement(1437..2030)
                     /gene="yhgN"
                     /locus_tag="ACHUU3_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR2083084.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWIKG"
     gene            2222..3325
                     /gene="asd"
                     /locus_tag="ACHUU3_003074"
     CDS             2222..3325
                     /gene="asd"
                     /locus_tag="ACHUU3_003074"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR2083085.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3598..5784
                     /gene="glgB"
                     /locus_tag="ACHUU3_003075"
     CDS             3598..5784
                     /gene="glgB"
                     /locus_tag="ACHUU3_003075"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR2083086.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5781..7754
                     /gene="glgX"
                     /locus_tag="ACHUU3_003076"
     CDS             5781..7754
                     /gene="glgX"
                     /locus_tag="ACHUU3_003076"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR2083087.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7772..9067
                     /gene="glgC"
                     /locus_tag="ACHUU3_003077"
     CDS             7772..9067
                     /gene="glgC"
                     /locus_tag="ACHUU3_003077"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR2083088.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9067..10500
                     /gene="glgA"
                     /locus_tag="ACHUU3_003078"
     CDS             9067..10500
                     /gene="glgA"
                     /locus_tag="ACHUU3_003078"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR2083089.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10519..12966
                     /gene="glgP"
                     /locus_tag="ACHUU3_003079"
     CDS             10519..12966
                     /gene="glgP"
                     /locus_tag="ACHUU3_003079"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR2083090.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3039 a         3225 c         3575 g         3157 t
ORIGIN      
        1 ttccaccagt gtcaaaatct cgacggggcg tttgttatta cccctccttt gatgcctggt
       61 ccggcgttgc tggttgacga tatcgtggat tctgcatgga cgctgacagt tctgacagca
      121 ctgttacgcc aggcaggttg cccgacggtt tatcctcttg cccttgcttc tacctcggta
      181 aaaaattgat gaatctttca gccaatgcac aagcaactct cctgctaacc agcgattttt
      241 ctcgcgcggc ggcgagtgag tataaacctc ttagtaatag tgaatggggg aagtttgcat
      301 tatggctgaa gcaccaacgt atcagtcccg ccgagcttct ggtgccgcaa ccgcaagaga
      361 aacttacagg ctggagcgat ccgcgtattt ctcaggagcg tattcttggc ttgctggcgc
      421 gtggtcatag tctggtgttg gcggtagata agtggcaacg cgccggttta tggatcttaa
      481 cccgcggaga tgctgattat cccgttcgct tgaaaaaccg attgcgaacg gatgcacctc
      541 ccgttttatt tggctgcggg aataaagcat tactgcaagc ggaaggtatg gcgattgttg
      601 gctcgcgaga tgctccgact gacgatttgc gctataccca acaactggcc gcgaaactgg
      661 cccaacaggg gatttgcgtt atctctggtg gtgcgcgagg tattgatgaa tgtgcaatgg
      721 cgtcggcact ggaggccggg ggaactgccg ttggcgtatt agctgatagc ttgttaaaaa
      781 cgagtacgtt agtgaaatgg cgtgaagggc ttatagcagg caacctggtg ttgatttcgc
      841 cgttttaccc agaggtacgt ttcaccgtcg gcaatgcgat ggcgcgaaat aaatatattt
      901 attgccttgc tgaaagcgca atggttgtac gtgcgggaat gaccggtgga acgataaccg
      961 gggcgatgga ggcattaaaa catcagtggc tgcctgtgca ggttaaacca aatcaggata
     1021 tgcaatcagc caattcacga ttagtagaaa atggggcgtc atggagtgct gaacaggctg
     1081 agaatgtgac gatcagactg ccagacgttc ctgggctgat gtatgacaga gcactccgta
     1141 acgctcaacc agaactgttt tcgctgcatg aagatgacgc aaattacgca gtaatgcccg
     1201 cgtatacgcc tgtcgatttt tatcaactct ttgtggcgga actggcgatc cttgcaaagg
     1261 aatcgataag tattgaaagg ctggcgtctt gtactggttt aaccatcgaa caaattagtg
     1321 tgtggctgaa ccgcgcagaa gaagagggaa gggttatccg attgggcgaa ggtcattatc
     1381 agttcaggta ataaaacagg tgccgcgtct ggttgtgtgc agcacctgtt tgaaccttac
     1441 ccctttatcc acattcgaat gccgtcgagg aacatctggg ttgccatcat caccagaatc
     1501 aatcccatca ggcgttcaag tgcgttcacc cctttctcgc ccagcagacg taaaaatagc
     1561 gaagactgta gcaggatgac aaaggtgccg ccccaggcca gcagcagagc aatcaccaga
     1621 tgtcccatct gattcgggta ctgatgagac aacaacatca gcgtggcgag aatagtcggc
     1681 ccggcgacta acggaattgc caacggcacg ataaatggct cttcacctgc cggaagcccg
     1741 ctgctatttc ctgaagcgct ggggaaaatc atcttaatgg cgattagaaa cagaatgatg
     1801 ccgccagaaa tggagacggt ttctgctcgc aggctgagaa atgccagaat tttctcaccc
     1861 gcaaacagga acaccagcat taccaggaga gcaataagca actctcgcac catgattgcc
     1921 cgccgtcttt tcggttcagt atgtttcagt acggacatga aaataggcag gtttccgagc
     1981 ggatccataa tcaggatcaa taaaactgct gcagaaatga tttcattcat aactcaaatt
     2041 ccctgataat tgccgcggac tttctgcgtg ctaacaaagc aggataagtc gcattactga
     2101 tggcttcgct atcattgatt aatttcactt gcgactttgg ctgctttttg tatggtgaaa
     2161 gatgtgccaa gaggagaccg gcacatttat acagcacaca tctttgcagg aaaaaacgct
     2221 tatgaaaaat gttggtttta tcggctggcg cggtatggtc ggctccgttc tcatgcaacg
     2281 catggttgaa gagcgcgact tcgacgccat tcgccctgtc ttcttttcta cttctcagct
     2341 tggccaggct gcgccgtctt ttggcggaac cactggcaca cttcaggatg cctttgatct
     2401 ggaggcgcta aaggccctcg atatcattgt gacctgtcag ggcggcgatt ataccaacga
     2461 aatctatcca aagcttcgtg aaagcggatg gcaaggttac tggattgacg cagcatcgtc
     2521 tctgcgcatg aaagatgacg ccatcatcat tcttgacccc gtcaatcagg acgtcattac
     2581 cgacggatta aataatggca tcaggacttt tgttggcggt aactgtaccg taagcctgat
     2641 gttgatgtcg ttgggtggtt tattcgccaa tgatcttgtt gattgggtgt ccgttgcaac
     2701 ctaccaggcc gcttccggcg gtggtgcgcg acatatgcgt gagttattaa cccagatggg
     2761 ccatctgtat ggccatgtgg cagatgaact cgcgaccccg tcctctgcta ttctcgatat
     2821 cgaacgcaaa gtcacaacct taacccgtag cggtgagctg ccggtggata actttggcgt
     2881 gccgctggcg ggtagcctga ttccgtggat cgacaaacag ctcgataacg gtcagagccg
     2941 cgaagagtgg aaagggcagg cggaaaccaa caagatcctc aacacatctt ccgtaattcc
     3001 ggtagatggt ttatgtgtgc gtgtcggggc attgcgctgc cacagccagg cattcactat
     3061 taaattgaaa aaagatgtgt ctattccgac cgtcgaagaa ctgctggctg cgcacaatcc
     3121 gtgggcgaaa gtcgttccga acgatcggga aatcactatg cgtgagctaa ccccagctgc
     3181 cgttaccggc acgctgacca cgccggtagg ccgcctgcgt aagctgaata tgggaccaga
     3241 gttcctgtca gcctttaccg tgggcgacca gctgctgtgg ggggccgcgg agccgctgcg
     3301 tcggatgctt cgtcaactgg cgtaatcttt attcattaaa tctggggcgc gatgccgccc
     3361 ctgttagtgc gtaatacagg agtaagcgca gatgtttcat gatttaccgg gagttaaata
     3421 gagcattggc tattctttaa gggtggctga atacatgagt attcacagcc ttacctgaag
     3481 tgaggacgac gcagagagga tgcacagagt gctgcgccgt tcaggtcaaa aaaatgtcac
     3541 aaccagaagt caaaaatcca attggatggg gtgacacaat aaaacaggaa gacaagcatg
     3601 tccgatcgta tcgatagaga cgtgattaac gcgctaattg caggccattt tgcggatcct
     3661 ttttccgtac tgggaatgca taaaaccacc gcgggactgg aagtccgtgc ccttttaccc
     3721 gacgctaccg atgtgtgggt gattgaaccg aaaaccgggc gcaaactcgc aaaactggag
     3781 tgtctcgact cacggggatt ctttagcggc gtcattccgc gacgtaagaa ttttttccgc
     3841 tatcagttgg ctgttgtctg gcatggtcag caaaacctga ttgatgatcc ttaccgtttt
     3901 ggtccgctaa tccaggaaat ggatgcctgg ctattatctg aaggtactca cctgcgcccg
     3961 tatgaaacct taggcgcgca tgcagatact atggatggcg tcacaggtac gcgtttttct
     4021 gtctgggctc caaacgcccg tcgggtctcg gtggttgggc aattcaacta ctgggacggt
     4081 cgccgtcacc cgatgcgcct gcgtaaagag agcggcattt gggaactgtt tatccctggg
     4141 gcgcataacg gtcagctcta taaatacgag atgattgatg ccaatggcaa cttgcgtctg
     4201 aagtccgacc cttatgcctt cgaagcgcaa atgcgcccgg aaaccgcgtc tcttatttgc
     4261 gggctgccgg aaaaggttgt acagactgaa gagcgcaaaa aagcgaatca gtttgatgcg
     4321 ccaatctcta tttatgaagt tcacctgggc tcctggcgtc gccacaccga taacaatttc
     4381 tggttgagct accgcgagct ggccgatcaa ctggtgcctt atgctaaatg gatgggcttt
     4441 acccacctcg aactactgcc cattaacgag catcccttcg atggcagttg gggttatcag
     4501 ccaaccggcc tgtatgcacc aacccgccgt tttggtactc gcgacgactt ccgttatttc
     4561 attgatgccg cacacgcagc gggtctgaac gtgattctcg actgggtgcc aggccacttc
     4621 ccgaccgatg actttgcgct tgccgaattt gatggcacga acttgtatga acacagcgat
     4681 ccgcgcgaag gctatcatca ggactggaat acgctgatct acaactatgg tcgccgtgaa
     4741 gtcagtaact tcctcgtcgg taacgcgctt tactggattg aacgttttgg tattgatgcg
     4801 ctgcgcgtcg atgcggtggc gtcaatgatt tatcgcgact acagccgtaa agagggggag
     4861 tggatcccga acgaatttgg cgggcgcgag aatcttgaag cgattgaatt cttgcgtaat
     4921 accaaccgta ttcttggtga gcaggtttcc ggtgcggtga caatggctga ggagtctacc
     4981 gatttccctg gcgtttctcg tccgcaggat atgggcggtc tgggcttctg gtacaagtgg
     5041 aacctcggct ggatgcatga caccctggac tacatgaagc tcgacccggt ttatcgtcag
     5101 tatcatcacg ataaactcac cttcgggatt ctctacaact acactgaaaa cttcgtcctg
     5161 ccgttgtcgc atgatgaagt ggtccacggt aaaaaatcga ttctcgaccg catgccgggc
     5221 gacgcatggc agaaattcgc taacctgcgc gcctactatg gctggatgtg ggcattcccg
     5281 ggcaagaaac tgctgttcat ggggaacgaa tttgcccaag gccgtgagtg gaaccatgac
     5341 gccagcctcg actggcatct gttggaaggc ggcgataact ggcaccacgg tgtccagcgt
     5401 ctggtgcgcg atctgaacct cacctaccgc caccataaag caatgcatga actggatttt
     5461 gacccgtacg gctttgaatg gctggtggtg gatgacaaag aacgctcggt gctgatcttt
     5521 gtgcgtcgcg ataaagaggg taacgaaatt atcgttgcca gtaactttac gccggtgccg
     5581 cgtcatgatt atcgcttcgg cattaatcag ccaggcaaat ggcgtgaaat cctcaatacc
     5641 gattccatgc actatcacgg cagtaatgca ggcaatggcg gcacggtaca cagcgatgag
     5701 attgccagcc acggtcgtca gcattcacta agcctgacac taccaccgct ggccactatc
     5761 tggctggttc gggaggcaga atgacacgac tcgccattgg caaacccgct cccctcggcg
     5821 cgcattacga cggtcagggc gtcaacttca cacttttctc cgctcatgcc gagcgggtag
     5881 agctgtgtgt ctttgacgcc aatggccagg aacatcgcta tgacttgcca gggcacagtg
     5941 gcgacatttg gcacggttat ctgccggatg cgcgcccggg tttgcgttat ggttatcgcg
     6001 ttcatggccc ctggcaaccc gccgaggggc atcgctttaa cccggcgaag ttgttgattg
     6061 atccttgcgc gcggcaaatt gaaggggaat tcaaagataa cccgctgctg cacgccggtc
     6121 ataatgaacc tgactatcgc gacaacgccg ccattgcgcc gaaatgcgta gtggtggttg
     6181 atcactatga ctgggaagat gatgccccgc cgcgcacgcc gtggggcagc accatcattt
     6241 atgaagccca tgttaaagga ttaacgtacc tgcacccgga gatcccggtc gagatccgtg
     6301 gcacttataa agccctcggg catccggtga tgatcaacta tttgaaacag ttgggcatta
     6361 ccgcgctgga actgctgccg gtggcgcagt ttgccagtga accacgtctg caacgcatgg
     6421 ggctaagtaa ctactggggt tacaacccgg tggcgatgtt tgcgctgcat ccggcgtatg
     6481 cctgctcgcc agaaacggcg ctgcatgagt ttcgcgatgc aatcaaagca ctgcataaag
     6541 cgggtatcga agtcattctt gatatcgtgc tcaaccatag tgcggaactg gacctcgacg
     6601 gcccgttatt ctcgctgcgt gggatcgaca accgtagcta ttattggata agagaagacg
     6661 gcgattatca caactggacc ggttgtggca acacgctcaa tttgagtcat ccggcggtgg
     6721 tggattatgc ctgcgcctgc ctgcgttatt gggtagaaac ctgtcacgtc gatggtttcc
     6781 gctttgatct ggcggcagtc atgggccgta cgccagagtt ccgtcaggat gcgccgttgt
     6841 ttaccgctat ccagaactgc ccggtgctct cgcaggtgaa gttaattgct gaaccgtggg
     6901 atatcgccct tggtggttat caggtgggaa atttcccgcc gctgtttgcc gagtggaacg
     6961 atcatttccg cgatgctgcc cgtcgtttct ggctacatta tgatttgcct ctgggggcgt
     7021 ttgccgggcg ttttgctgcc tccagcgatg tttttaaacg taatggtcgt ctgccgagtg
     7081 ccgcgattaa tctcgtcact gcgcatgacg gttttacgct tcgcgactgc gtttgcttca
     7141 accataaaca caatgaagca aacggagaag aaaatcgcga cgggaccaac aacaattaca
     7201 gtaacaatca tggtaaagaa gggttaggcg gttctcttga cctggttgaa cggcggcgcg
     7261 acagtattca cgccctgtta acaacgttgt tgctctccca gggtacgccg atgttactgg
     7321 ccggtgacga acacggtcac agccagcatg gcaataacaa tgcctactgt caggataacc
     7381 aattaacctg gttggactgg tcgcaggcaa gcagtggttt aaccgcattt accgccgcgt
     7441 taatccatct gcgcaagcgc attcccgctt tggtggagaa tcgctggtgg gaagaaggcg
     7501 acggcaatgt ccgttggcta aatcgatatg ctcaaccttt aagcacggat gagtggcaaa
     7561 acgggccgaa acagctgcaa attctgctct cggatcgctt tttgatcgca attaacgcca
     7621 cgcttgaggt aacagagatt gttttacctg ctggggagtg gcacgccatt cccccattcg
     7681 ctggagagga taacccagtg attacggctg tctggcaggg acctgcacac ggattgtgtg
     7741 tgttccagag atgataaaaa aggagttagt catggttagt ttagagaaga acgatcactt
     7801 aatgttggcg cgccagctgc cattgaaatc tgttgccctg atactggcgg gaggacgtgg
     7861 tacccgcctg aaggatttaa ccaataagcg agcaaaaccg gccgtacact tcggcggtaa
     7921 gttccgcatt attgactttg cgctgtctaa ctgcatcaac tctgggatcc gtcgtatggg
     7981 cgtgatcacc cagtaccagt cccacactct ggtgcagcac attcagcgcg gctggtcatt
     8041 cttcaatgaa gaaatgaacg agtttgtcga tctgctgcca gcacagcaga gaatgaaagg
     8101 ggaaaactgg tatcgcggca ccgcagatgc ggtcacccaa aacctcgaca ttatccgccg
     8161 ttataaagcg gaatacgtgg tgatcctggc gggcgaccat atctacaagc aagactactc
     8221 gcgtatgctt atcgatcacg tcgaaaaagg cgcacgttgc accgttgctt gtatgccagt
     8281 accgattgaa gaagcctccg catttggcgt tatggcggtt gatgagaacg ataaaattat
     8341 cgaattcgtt gaaaaacctg ctaacccacc gtcaatgccg aacgatccga gcaaatctct
     8401 ggcgagtatg ggtatctacg tctttgacgc cgactatctg tatgaactgc tggaagaaga
     8461 cgatcgtgat gagaactcca gccacgactt tggcaaagat ttgattccca agatcaccga
     8521 agccggtctg gcctatgcgc acccgttccc gctctcttgc gtacaatccg acccggatgc
     8581 cgagccgtac tggcgcgatg tgggtacgct ggaagcttac tggaaagcga acctcgatct
     8641 ggcctctgtg gtgccggaac tggatatgta cgatcgcaat tggccaattc gcacctacaa
     8701 tgaatcatta ccgccagcga aattcgtgca ggatcgctcc ggtagccacg ggatgaccct
     8761 taactcactg gtatccggcg gctgtgtgat ctccggttcg gtggtggtgc agtccgttct
     8821 gttctcgcgc gttcgcgtga attcattctg caacattgat tccgccgtat tgttaccgga
     8881 agtatgggta ggtcgctcgt gccgtctgcg ccgctgcgtc atcgatcgtg cttgtgttat
     8941 tccggaaggc atggtgattg gtgaaaacgc agaggaagat gcacgtcgtt tctatcgttc
     9001 agaagaaggc atcgtgctgg taacgcgcga aatgctacgg aagttagggc ataaacagga
     9061 gcgataatgc aggttttaca tgtatgttca gagatgttcc cgctgcttaa aaccggcggt
     9121 ctggctgatg ttattggggc attacccgca gcacaaatcg cagacggcgt tgacgctcgc
     9181 gtactgttgc ctgcatttcc cgatattcgc cgtggcgtga ccgatgcgca ggtagtatcc
     9241 cgtcgtgata ccttcgccgg acatatcacg ctgttgttcg gtcattacaa cggggttggc
     9301 atatacctga ttgacgcgcc gcatctctat gatcgtccgg gaagcccgta tcacgatacc
     9361 aacttatttg cctataccga caacgtattg cgttttgcgc ttctggggtg ggttggggca
     9421 gaaatggcca gcgggcttga cccattctgg cgtcctgatg tggtgcatgc gcacgactgg
     9481 catgcaggcc ttgcgcctgc gtatctggcg gcgcgcgggc gtcctgcgaa gtcggtgttt
     9541 actgtgcaca acctggccta tcaaggcatg ttttatgcac atcacatgaa tgacatccaa
     9601 ttgccatggt cattctttaa tattcatggg ctggaattca acggacaaat ctctttcctg
     9661 aaggccggtc tgtactatgc cgatcacatt acggcggtca gtccaaccta cgctcgcgag
     9721 atcaccgaac cgcagtttgc ctacggtatg gaaggtctgt tgcaacagcg tcatcgtgaa
     9781 gggcgtcttt ccggcgtact gaacggcgtg gacgagaaaa tctggagtcc agagacggac
     9841 ttactgttgg cctcgcgcta cacccgcgat acgttggaag ataaagcgga aaataagcgc
     9901 cagttacaaa tcgcaatggg gcttaaggtt gacgataaag tgccgctttt tgcagtggtg
     9961 agccgtctga ccagccagaa aggtctcgac ctggtgctgg aagccttacc gggtcttctg
    10021 gagcagggcg ggcagctggc gctactcggc gcgggcgatc cggtgctgca ggaaggtttc
    10081 cttgcggcgg cagcggaata ccccggtcag gtgggcgttc agattggcta tcacgaagca
    10141 ttttcgcatc gcattatggg cggcgcggac gtcattctgg tgcccagccg ttttgaaccg
    10201 tgcggcttaa cgcaacttta tggattgaag tacggtacgc tgccgttagt gcggcgcacc
    10261 ggtgggcttg ctgatacggt ttctgactgt tctcttgaga accttgcaga tggcgtcgcc
    10321 agtgggtttg tctttgaaga taataatgcc tggtcgctgt tacgggctat tcggcgtgct
    10381 tttgtactgt ggtcccgtcc ttcactgtgg cggtttgtgc aacgtcaggc tatggcaatg
    10441 gattttagct ggcaggtcgc ggcgaagtcg taccgtgagc tttactatcg cttgaaatag
    10501 ttttcaggaa acgcctatat gaatgctccg tttacatatt catcgcccac gcttagcgta
    10561 gaagctctta agcactctat cgcttacaag ctgatgttta cgattggaaa ggacccggtc
    10621 gtcgccaata aacatgaatg gctgaacgca acgttatttg ctgtgcgcga tcgtctcgtg
    10681 gagcgctggt tacgttcaaa ccgtgcccag ttgtcgcaag aaacacgtca ggtttactac
    10741 ctgtcgatgg agtttttgat tggccgtacg ctctccaacg ccatgttgtc gctaggaatt
    10801 tacgaagatg tacagggcgc actggaagcg atggggttaa atctcgaaga gctgattgat
    10861 gaagaaaatg acccaggcct cggtaacggt ggcctgggac gtctggcggc ttgcttcctt
    10921 gattctctgg cgacgttagg gttgccgggg cgcggttacg gcatccgcta tgactacggt
    10981 atgttcaagc agaacatcgt taacggtagc cagaaagagt cgccagacta ctggctggaa
    11041 tacggtaacc cgtgggaatt caaacgccac aacacgcgct ataaagtccg ttttggcggt
    11101 cgcattcagc aggaaggtaa aaaaacgcgc tggattgaaa ccgaagagat tctgggagtc
    11161 gcttacgatc agataatccc tggttacgac accgacgcga ccaacacgct gcgtttgtgg
    11221 agtgcgcaag ccagtagcga aattaacctc ggtaaattca accagggtga ctacttcgcg
    11281 gcagtggaag ataaaaacca ctccgagaac gtatctcgcg tactgtatcc ggatgactcc
    11341 acctactccg ggcgtgagct gcgcctgcgt caggaatact tcctggtttc ctcgaccatt
    11401 caggacattt taagccgcca ttatcagttg cataaaacct acgataacct ggcggataaa
    11461 atcgcgattc atctcaatga tacccatccg gtactgtcga ttcctgagat gatgcgtctg
    11521 ctgatcgatg agcaccaatt tagctgggac gacgcgtttg aggtgtgttg tcaggtcttc
    11581 tcctacacta accacacgct gatgagcgag gcgctggaaa cctggccggt tgatatgctg
    11641 ggtaaaattc tgccgcgtca cctgcagatc atctttgaaa tcaacgacta tttcctgaaa
    11701 accttgcagg aacagtatcc gaacgatacc gatctgctgg gacgggcgtc gatcattgat
    11761 gaatccaacg gtcgtcgtgt gcgtatggcc tggctggcgg ttgttgtgag ccacaaagtt
    11821 aacggtgtat cggaactgca ctctaatctg atggtgcaat cgttgtttgc cgactttgcg
    11881 aaaatcttcc cgggtcgttt caccaacgtc accaacggtg tgacgccgcg tcgctggctg
    11941 gcggtagcga acccatcgct ttcagccgtg ctggacgaac acctgggccg taactggcgc
    12001 accgacctta gcctgcttaa tgagctgcaa caacactgtg atttcccaat ggttaatcac
    12061 gctgtgcatc aggcgaagct ggagaacaaa aagcgtctgg cagagtatat cgcccagcag
    12121 ctgaatgtgg tggtgaatcc aaaggcgttg ttcgatgtac aaatcaaacg tattcacgaa
    12181 tacaaacgtc aattgatgaa tgtgttgcat gtgattaccc gctataaccg catcaaggcc
    12241 gacccggatg cgaagtgggt accgcgcgtg aatatttttg gcggtaaggc ggcttcggcc
    12301 tattacatgg cgaagcacat tattcatttg atcaatgacg tagcgaaagt gatcaacaac
    12361 gatccgcaga ttggcgataa gctgaaagtc gtgttcatcc cgaactacag cgttagcctg
    12421 gcgcagttga tcattccggc ggcagatctg tctgaacaga tttcgctggc agggacggaa
    12481 gcttccggca ccagtaacat gaagtttgcg cttaacggtg cgctgactat cggtacgttg
    12541 gacggtgcga atgtcgagat gctggatcat gtcggtgctg acaatatctt tatttttggt
    12601 aacacagcgg aagaagtgga agaactgcgt cgtcagggct acaaaccgcg tgaatactac
    12661 gagaaagatg aggagctgca tcaggtgctg acgcaaatcg gcagcggtgt attcagtccg
    12721 gaagatccgg gtcgctatcg cgatctggtt gattcgctga tcaacttcgg cgatcactat
    12781 caggttctgg cggattatcg cagctatgtc gattgtcagg ataaagtcga cgaactctac
    12841 gagcttcagg aagagtggac cgcaaaagcg atgctgaaca ttgccaatat gggctacttc
    12901 tcttctgacc gtactatcaa agagtacgcc gatcatatct ggcatatcga tccggtgaga
    12961 ttgtaagttc accaataaat agaacggggc caaagg
//
LOCUS       DAZPLI010000116        12941 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZPLI010000116 DAZPLI010000000
VERSION     DAZPLI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12941
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.117"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..243)
                     /locus_tag="ACHUU3_003080"
     CDS             complement(<1..243)
                     /locus_tag="ACHUU3_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR2083091.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
     gene            complement(253..1629)
                     /gene="hpaX"
                     /locus_tag="ACHUU3_003081"
     CDS             complement(253..1629)
                     /gene="hpaX"
                     /locus_tag="ACHUU3_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR2083092.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            complement(1804..2592)
                     /gene="hpaI"
                     /locus_tag="ACHUU3_003082"
     CDS             complement(1804..2592)
                     /gene="hpaI"
                     /locus_tag="ACHUU3_003082"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR2083093.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            complement(2603..3406)
                     /gene="hpaH"
                     /locus_tag="ACHUU3_003083"
     CDS             complement(2603..3406)
                     /gene="hpaH"
                     /locus_tag="ACHUU3_003083"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR2083094.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            complement(3517..3897)
                     /locus_tag="ACHUU3_003084"
     CDS             complement(3517..3897)
                     /locus_tag="ACHUU3_003084"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR2083095.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            complement(3907..4758)
                     /gene="hpaD"
                     /locus_tag="ACHUU3_003085"
     CDS             complement(3907..4758)
                     /gene="hpaD"
                     /locus_tag="ACHUU3_003085"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR2083096.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            complement(4760..6226)
                     /gene="hpaE"
                     /locus_tag="ACHUU3_003086"
     CDS             complement(4760..6226)
                     /gene="hpaE"
                     /locus_tag="ACHUU3_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR2083097.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            complement(6223..7512)
                     /gene="hpaG"
                     /locus_tag="ACHUU3_003087"
     CDS             complement(6223..7512)
                     /gene="hpaG"
                     /locus_tag="ACHUU3_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR2083098.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            7784..8230
                     /gene="hpaR"
                     /locus_tag="ACHUU3_003088"
     CDS             7784..8230
                     /gene="hpaR"
                     /locus_tag="ACHUU3_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR2083099.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            8349..10004
                     /gene="tsr"
                     /locus_tag="ACHUU3_003089"
                     /pseudo
     CDS             8349..10004
                     /gene="tsr"
                     /locus_tag="ACHUU3_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(10053..11414)
                     /locus_tag="ACHUU3_003090"
     CDS             complement(10053..11414)
                     /locus_tag="ACHUU3_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2083100.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLMRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(11629..12543)
                     /locus_tag="ACHUU3_003091"
     CDS             complement(11629..12543)
                     /locus_tag="ACHUU3_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR2083101.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            12682..>12941
                     /gene="lgoD"
                     /locus_tag="ACHUU3_003092"
     CDS             12682..>12941
                     /gene="lgoD"
                     /locus_tag="ACHUU3_003092"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFR2083102.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAV"
BASE COUNT         2999 a         3377 c         3537 g         3028 t
ORIGIN      
        1 tgacggcggc gtcaggacaa acaggggcgc ttccaccgag tagcgatgat cgtcaagctg
       61 tagctcaatc tgtccgctat tgaggaaatg catctgaaag tactgttcgt ggcgatgtgg
      121 cagcatatgg cggccaaaaa atgccgccat gcgggcgaac gactgataat gcacatcgtc
      181 cgtgcccagg ctttcatcgt actctttgct gatatcaata ttggcaatct gacggtcaca
      241 catcgtcgtt ccttacaggg tcgctcgcgg acgggaggac tgcattggaa tcgcccagat
      301 aatccccgca ccaatcacca gcagcgcggc aacaaaccac aatccactgt taaagctgcc
      361 ggtcaaatct ttcaaccagc cgatcataaa cggacttaac gctgaaccaa tgttgccagt
      421 ggcgttgatc accgcaatac cgatcgctcg tgcccgcagg ctgatggact gatccggtgt
      481 tgtccagaaa atcgccattg cgctgaatga tccggtcgaa gccataatga tccccagcat
      541 ctggatcatg ttgtgatcag ttgccgaagc cagtaaccaa cctgcggcag cgaacaaata
      601 aggaagggcg gtgtgatgcc ttcgttcctg gcggcgatct gagtgacggc tccagtagac
      661 catcccgaga atggtacaaa tctgcggtac ggcggccagc atgccgatgg tgatattact
      721 gctgccctga ttaaaacttt gcaggatctg cggtgtccag atgctgatcg cactaagtgt
      781 gttggtcagg cagaaatacg ccagggtata catcatcacc accggagtga agatctcccg
      841 ccacatactg cgttgttgca tggcgtggtg gctgatggct ccctctggct gaaccagcgt
      901 cagacgatcg ttatccatca tctcttgcag gcattttttg tcttctttcg tcagccactt
      961 agctttgtcc ggtgagtcat caagccagaa ccacaccatg acgccgagta ataccgacgg
     1021 gaagccttcc agcaaaaaca gccactgcca gccttttaat gccatgacgc catccagcga
     1081 caaaatgtag ccggaaacga tcgagcccaa cgctgtcgtt accggcattg ctaccataaa
     1141 caaggcgttg gcacgggcgc ggaaataggc cggaaaccag aaggttaaat acagcagaat
     1201 accaggcaga aagccggctt cggtaatgcc aaccagtata cgcagtacgt acaagctggt
     1261 ggggccagtg gcaaacatgg tggcagtaga ggcgatgccc cagagcacca tgatggtggc
     1321 gatccagcgc cgcgcaccga caatactcag cataatgttg ctgggaatgc cgaagatgac
     1381 ataagcggca tagaacaggg tggtagcgag gccaaacatg gtggcgctca gaccgaggtc
     1441 gcgtcccatc gtgagtccgg caaagccgat gttgatgcga tcaaggaacg agaagataaa
     1501 cagcacgaac agaaagacgc tcaggcggcg aaatagcttc ttaataaccg cctgttgtcc
     1561 ggcagtcagc gctttatgct gattcgccgg atcgatactc tccggtatgg caggtgaggt
     1621 gtcgctcatt tttcttcctc taatgggatt tttgtagggt acgacaaggt ttatggcgga
     1681 tgcggcgtaa cgccttatcc ggtccgcgat ttggtacggc tttttgccgg atgcggcgta
     1741 aacgccttat ccggcctacg gtttggtgcg agtgtaggcc ggataagcgc agcgcatccg
     1801 gcattaatac acgccgggct tcacggcggt ggcctgcgca ccaaaccgtg ctgccagcgc
     1861 ttcggcggcg cgggcgagca gggtggtgtc aacgccgacg gcgacaaaca gcgcgcccag
     1921 ttccagatag cgttttgcca gttgctcatt ggcgatcagg atccccggcg ctttgcccgc
     1981 ttcgcggatc tgcacgatcg cctgctcaat ggcggcctgt acttccgggt gctgcggatt
     2041 accggcataa cccatatcgg cgctcagatc cgccgggccg ataaacacgc cgtcgacgcc
     2101 ttccacgtcc agaatctgcg gtaagttctt cattgcctca cgcgtttcga tctgcaccag
     2161 cacgcacatt tgatcgttgg ctttttgcag gtaatcagga atacgattcc agcgcgaggc
     2221 gcgggccagc gcactgccca caccgcgaat accggcgggg ggataacggg tggcgcgtac
     2281 cgcttcacgg gcttcgtcag cgttttgtac catcggcacc agcaaggttt gtgtgccgac
     2341 gtccagcagt tgtttgattt gcaccggatc gttccacgac ggacgtacta ccggctggct
     2401 ggggtagggc gcaatcgcct gtagctgggt gagcacggtt tgcacgttat tcggcgtgtg
     2461 ctcaccgtcg atcaacaacc agtcgaatcc tgctccggcc agtaactctg cgctgtagct
     2521 gctactcagc cccagccata atccgatctg cggacggcct gctttcagcg ccgctttaaa
     2581 gctgttttcc atcgttctct ccttaaacaa agcggcagct aatggaaccc atgttgccgt
     2641 aatcgacgtg gaaggtgtcg cccttacgcg ccggaaccgg gcgggtgaac gaaccaccga
     2701 gaatgatttg cccggcttcc agttgtacgt catacggtgc gagtttgttc gccagccagg
     2761 ccacgccgtt tgccggatga ttcagcacgc cagcggcgac gccggtttct tcaatcacgc
     2821 cattgcgata catcagggcg gagatccaac gcagatccag ttcgtctggt ttgatcggac
     2881 gaccgccgag gatcaccccg gcgttggcgg cgttatcaga aatggtgtcg aacactttgc
     2941 gcgggcgctg ggtttccgga tcgatgttgt ggcagcgagc gtcgatcagc tccagcgccg
     3001 ggatcacgta gtccgtggcg ttgtagacgt cgaacagcgt gcagtttggc ccacgcagcg
     3061 gttttgccag cacgaaagcc agctccactt caatgcgcgg cacgataaag cgatcggtcg
     3121 ggatatcgct gccatcgtgg aagaacatgt catccagcag cgcgccgtaa tccggttcgc
     3181 tgatctgcga gcttgcctgc atcgctttcg aggtcaggcc gattttgtgg cctttcagcg
     3241 tgcgaccttc ggcgattttc aggcgaaccc attcacgctg caccgcgtaa gcgtcttcga
     3301 tggtgatctc cgggtaatcc agcgagatcg cgcggatctg ttcgcgctgt ttttctgcct
     3361 gatccagacg ctgggcgatc agggtgtggg tgtgtttatc gaacatagcg atatcctgtg
     3421 gcattgccgg atgcggctgc gccttatccg gcctacggtg tacggtttgg ggttttgtag
     3481 gcccggtaag cgcagcgcca ccgggcaatc tgcgcgctac ttaaacaatg cgtgcacgtt
     3541 gttttgttta aaattcagcg tcggatgcag ctcttcaatc tcaaacgaca acgccagcag
     3601 gcggctctcc atcagggcgg cgaagtgcgt tttaatcagt tcaaacagca tttcacccgc
     3661 ctgctggcgg ctttccaggc tgcgacctgc gccgattttc aacgtcatat gcacgaaggc
     3721 ataatcacgc tgcccgtcgg ccatctgcca ggtatcgacc caatgcacgc ggctgcgaat
     3781 acccgccagc ggaaaaatac ccgtggctgc cagcgtcgga ttcactttgg cgaacaaccc
     3841 cggcaggtcg gcttcttcgc ggatgttgtc actgcattta acgataaagt gcggcataaa
     3901 acgtccttac gcgggaagcg ggaaaacagc gttaacctga ccggtgccag agcttgggaa
     3961 tagctcggtg ataaactcca ccttgccgtc gtatttatcc cagccgagca tccccagcag
     4021 catcaccgtg tcgtgcatat tgccttcgcc gtagcagtag tcggcgtact ccggcagcat
     4081 attgcagaac tctttgaact ggccttcgcg ccacaacttc accacgcgct cgtccatctg
     4141 gcggtcgaac tcgcgggtgt agctgttcat cccttcttcc gcacgctgat cgtcaataaa
     4201 gcggtgcgat aacgaaccgc tggcaaggac cgccacggtg ccgtcgtact gttcgatcgc
     4261 tttcagaatc gcttcgccca gcttgcggct gtcggcaaaa tcgtgaaccg tgcagaaagc
     4321 cgaaatggag accactttga agtgcttgtc ttcattcatg tagcgcatcg gcaccagcgt
     4381 gccgtattcc agtttcaggc tgggaatgtt gtgcgctttt gcccgcacgc cgagctttaa
     4441 ggcttcatcg gcaataagct gccccaactc cgggttgccc tcgtagttgt aggtcatatc
     4501 gcggataaaa tgcggcagct cgttgctggt gtagacgcct tcaaaatggt ctgcacagtt
     4561 gatgtgataa gcactgttga ccagccagtg ggtatcgaaa acgataatgg tatcgacgcc
     4621 catttcccgg caacgcttgc tgatctcttt atgcccgtcg atcgcgccct ggcggcaacc
     4681 gtggtttttc cctggcagtt cagagtgata catcgacggt acgtgcgtga tcttggcggc
     4741 taacgctaac ttacccatat cagactcccc atttcggaat tggatggtcg cccatggaaa
     4801 tgcagacgtt cttcatttcc gcgaacactt cgaagctgta ctcaccgcct tcacgcccgg
     4861 tgccggaggc ttttacgccg ccaaatggct ggcgcaggtc acgcacgttc tgggtgttga
     4921 cgaacaccat gccagcttca atgccacgcg ccaggcgtaa cactttgctg acatcctgtg
     4981 tccagatgta cgacgcgagg ccgtactcca cgtcgtttgc cagacgtaag ccttcggctt
     5041 cgtctttaaa cggcagcagg caggcgaccg gcccgaaaat ctcttcctgg gcgactcgca
     5101 tacggttatc aacgtctgcc agcacggttg ggcgcaggaa gttgccgcct ttcaggtgcg
     5161 caggcaggtc ggacggttta tccgggccgc ccgccagcag ggttgcgcct tcttcaatgc
     5221 cgagacggat atagccggag actttttccc agtgctgctg gctaataagc gcgccaacct
     5281 gggtattcgg atcgttcgga tcgcccacgc gcagacggtt ggcgcgttcg gcaaagcgtt
     5341 taacgaattc cgggtagatg ctttgctgaa taaagatgcg cgaaccggcg gtgcagcgtt
     5401 cgccgttgat cgagaagatg gtgaacaggg cggcgtccag cgcgcgttca atatcggcat
     5461 cttcaaaaat cagcaccggc gatttaccgc ccagttccat ggagtatttt ttcagcccgg
     5521 cgtttttcat gatgttgcgc ccggtggccg taccgccggt gaacgacacg gcacgtacgt
     5581 catgatgacg aaccagcgca tcccctgcgg ttgcgccgta gccctgtacc acgttcagca
     5641 cgcctgccgg aatatcggct tccagcgcca gctcacccag gctgtcagcg gtcagcgggg
     5701 agagttccga cattttcagt accgcggtat tgcccagcgc cagacacggc gcgaccttcc
     5761 atgtggcggt cataaacggt acgttccacg gcgataccag cgcacaaacg cccaccggct
     5821 gcaccagcgt gtagttgagc atcttgtcgt caaccggata ggtcttgccg ttcatctgct
     5881 ggcagacttc cgcgaaaaat tcaaagttgt gggaagcgcg tgggatcaac acatttttgg
     5941 tctgatggat cggcaggccg gtgtccgcgg tttccatcgc ggcgatctct ggcacgttct
     6001 ggtcgatcag atcgcccaga cggcgcatca ggcgcgcacg ctctttcatc ggcagattgg
     6061 cccatttcgg gaacgcctct ttcgctgccg ctaccgcctg attgatctcc gcttcaccgc
     6121 cagaggccac atccgccagc acttcacccg ttgccggatt ggtggtctgg aagtagtcgt
     6181 tacctgcaac atttttgccg ttgatccaat gatttacttt tttcatttcg ctgtatcctc
     6241 actcacaatt cggttcacca ggcggcccac gccttctact tccaccacta cttcatcgcc
     6301 aggcaccacg tcagataagc ctttgggtgt gccggtggcg atcatgtcgc ccggattcag
     6361 ggtcataaat tcacttaagt aggcgatcag gaagggcacg ctgaagatca gatcggcggt
     6421 ggtgccttgc tggcgtaact cgccgttgac gaaggtgcga agggtcagat tatgcgggtc
     6481 cgggatcgcc tctttcggca cgatggttga aagcatcggc gtcagtccgt cgcggctttt
     6541 tacccgcagg ttagggcggt agtagttttc cagatagtca cgaatggcgt agtcgttaca
     6601 cacggtgtag cccgcgacat aatccatggc atcggcttcg ctgacgttac gcgcctgttt
     6661 gccaataact accaccagct ccgcttcata gtgcatgtat tcaatattgt ttggacgcac
     6721 ggaggtctgg ttatcgccag tgagggtatt tggcgctttc aggaacacca gcggttcttc
     6781 cggtggctta aattccagtt cgctggcgtg gtcggcgtag ttcaggccga gggcaaacag
     6841 cgtgccgtgc gggtgtggct gcgttgggaa gctcttgtgc gtggtcactt cacgttcgtc
     6901 cactaccgga ttttccagcg gcgggaaacc ttctgcgaga acacgaacgc gatcgcctgg
     6961 ctgtatttcc acgcgcgcct gtggcgtgcc gagcagaatg gcgtcgcctg gattcagcgt
     7021 ggcaaattcg ctcagggcgc tcagcaactg cgcggcgtta cgttgtaaat cggcggtatt
     7081 ccagtgatcg gcaggacgcc cgttgatctc ggtatagatg gtcagattat cgacattgct
     7141 gagagccacg gtttcgccaa tggggcagaa tccatcacga cattttgctt tgattgccgg
     7201 gcggtaaaag ctctcttccg gcaggctgac gtcgttagcc agcgcatatc cggcgatgta
     7261 ctctgccgca tcttcttcac gtactttcgt cgccgttttt cccacaatca gcgcaacagt
     7321 cgcaccgctc agtacctttt caccctgtgg aaagggaatc ggttcaccgc aaccaatcac
     7381 cgtattgcgc ggtttaataa accagaccgc agttttaggc ggggctttgt agggggattg
     7441 ctggaacgct tcctgccatg catcaagttg gctgcgatgg ttcaacgcta cggcgaagat
     7501 agtgcctttc attaccactc ctcggattcg atcatcttaa tcattaatat gttaatgatt
     7561 gcttttattc ccgattgcgt agtttggcaa aagtaagtga tgtaaatgtg atctaattaa
     7621 caaactattt acatttaaag ataataaaat attaaatatc agtaagttaa taaaatatca
     7681 tcaattttga taaaatcagg aattgcaggg gcagcgcagt cccggtaacg tgacgattaa
     7741 ctgtttctat attaatgatt ttgaattttt caggggaaag ataatgcacg actcactaac
     7801 cattgcgttg ctccaggcgc gagaagcggc gatgagttat tttcgcccaa tcgttaaacg
     7861 gcataatttg actgagcaac agtggcgcat cgtgcgtatt ctggcggaaa gcccatcaat
     7921 ggattttcac gatctggcgt atcgcgcctg cattttgcgc ccaagcctga ccgggatcct
     7981 cacgcggatg gaacgcgacg gtttagtgtt gcgattaaag ccgatcaatg atcagcggaa
     8041 gctctatatc tcgctaacca aagaggggca ggcgctgtat aaccgcgctc agacacagat
     8101 tgaagaagcc tatcggcaga ttgaagctca gtttactgcc gagaaaatgc aacagttaac
     8161 ccatctgtta gaggaattta ttgctctggg caattcccga caagaagcca tcccgggcga
     8221 taatgaataa ctttttagta tctgataacc agcgcctggt ttttccatgg atggtgggtt
     8281 acaccttttc ataaagtttt tgctttccag gccgaaaatc ttgcatcggt ccacaggaaa
     8341 gagaaaccat gttaaaacgt atcaagatcg tgaccagctt actgctggtt ttggccgttt
     8401 ttggcctttt acaactgaca tcaggcggtc tgttctttaa tgccttaaag aatgacaaag
     8461 aaaatttcac tgttttacaa accattcgcc agcagcaatc cacgctgaat ggcagctggg
     8521 tcgcgttgtt gcagacgcgt aacaccctca accgcgcggg tatccgctac atgatggatc
     8581 agaacaatat tggtagcggt tcaaccgttg ctgagctgat gcagagtgcc agtatttcgc
     8641 tgaaacaggc ggaaaaaaac tgggcggatt acgaagcgtt gccgcgtgac ccgcgtcaga
     8701 gcaccgccgc agcggcagag atcaaacgta attacgatat ttatcacaat gcgctggcgg
     8761 agctgatcca actgttaggt gcaggcaaaa tcaacgagtt ctttgatcag ccgacccagg
     8821 gatatcagga cggtttcgag aagcagtatg tggcttacat ggagcaaaac gatcggctct
     8881 atgatatcgc cgtcagcgat aacaatgcct cctacagcca ggcgatgtgg attctggtgg
     8941 gcgtgatgat cgtcgtactg gcggtcatct tcgccgtctg gttcggtatt aaagcctcgc
     9001 tggtagcgcc aatgaatcgc ctgattgaca gcattcgtca tattgcaggc ggcgatctgg
     9061 tgaaaccgat tgaggtggat ggctctaatg agatggggca actggcagag agtttgcgcc
     9121 atatgcaggg agagctgatg cgtaccgtcg gtgatgtgcg caacggggcc aatgccatct
     9181 atagcggtgc cagcgaaatc gccaccggca ataacgatct ctcttcgcgc accgagcaac
     9241 aggccgcttc gctggaagag acggcagcca gcatggagta actgaccgca acggtgaagc
     9301 agaacgccga aaatgcgcgc caggccagcc atctggcgtt aagtgcttct gaaacggcgc
     9361 aacgcggcgg caaagtggta gataacgtgg tgcagactat gcgcgatatc tccaccagtt
     9421 cgcagaaaat cgccgatatt atcagcgtaa ttgacggcat tgccttccag accaatattc
     9481 tggctttgaa cgcggcggtt gaggctgcgc gtgcgggtga gcaagggcgc ggttttgcgg
     9541 tggtcgcggg agaagtgcgt aatctggccc agcgcagcgc ccaggcggct cgtgaaatta
     9601 aaagcctgat tgaagactcg gtggggaaag tggatgttgg ctctacgctg gtcgaaagcg
     9661 ccggggaaac aatggcggag attgtcagcg ccgtgacccg cgtgacggac attatgggcg
     9721 aaattgcttc tgcttctgat gagcagagcc gtggtatcga tcaggttggc ttagcggttg
     9781 ctgagatgga ccgggtaact caacagaacg ccgcgctggt ggaagagtct gccgctgccg
     9841 ccgccgcgct ggaagagcag gccagtcgcc tgaccgaagc agtggcagtg ttccggattc
     9901 agcaacagca gcgtgaaaca tcggctgtgg taaaaaccgt gacgccagct gcgccgcgta
     9961 aaatggccgt ggcagatagc gaggagaact gggaaacatt ttaatcgcca tgaaaatgcc
    10021 cgataagcaa aatgttatcg ggcataagga gattaatctt tacgtgggtc gttgatcggc
    10081 tgacgaacca ggaagatgta cgccagtgcc cccgctgcgg tgacgcaacc gcagatgatt
    10141 agtgccagac ggaatgagtg ggtggtatca acaataaaac cagtaatgat cggcgcaaaa
    10201 gaggcgcaga tgaagctggc aaagttctgg atactgccca ccgacgcagt catgcgagaa
    10261 gcaactgcga cgtggatcaa gccccagcag gatgttccgg caaagtgaat acagaacagt
    10321 gccatgccaa tcagcagaac cgctgtcatg gatgttgtcg cttgcggtac gaccagcgta
    10381 aaggcggcag aacagaacat cccggcaata atgcagatct tacggctttt aatcggagcc
    10441 attccccctt tgaccagcca gtcagtaacg taaccgttga ccagcatccc ggcagcccca
    10501 aacaggaaag ggatagccgc catcaaccct gtgcttttta aatccaggtt ataggctgtt
    10561 tgcaggtaac caggaagcca ggccagatac agccacgcag tgtagttgat gccactgaat
    10621 ccgagcatca ttccccacat tgtacggtta cggaacaggc tgcgccattc ggcaaaactg
    10681 agcggatctc ggcgggcatt gacgctacct gcattgagat aagcttgttc aacggcagtc
    10741 agttctacgt gctcgcggtt gcgatagagc atataccagc cgatggcgag aaaaatcccc
    10801 agtacaccaa tggtaataaa catcccgcgc cagcccatca ccagcatcat cgccgccaga
    10861 atcggtgggc ttacggcaac gccaatggta gaagctgcgt tgaagaagcc catcgggcgt
    10921 ccgcgctctt tgatgttgaa ccagtcgtta atgactttta caccgcatgg gttcatcggc
    10981 gcttcaccaa tccccatacc gatacgcatc aacacgaact gcgtaaagtt gtgcaccatg
    11041 ccagacattg cctggaacag tgaccagaag aacatcccca gtcccagcat caggcgtggg
    11101 cctttacgat ccaatagtgg gccgcaagga agttgcgcaa tcccgtaagc gagtgaaaac
    11161 acggagagca aagcgccgat ttcggtggca cttaatccca attcttcacg aatcgttaaa
    11221 tttgctaccg acagcgaact gcggtcgaga taattgatta ccgccgcaaa aaataataac
    11281 aacatggcgg tggtttgaat gcgtttaatt cgggtactgc gttgaactaa tccatctggt
    11341 ggcacgggaa tatctgccga cgaagatgga gtaaatgaag aacgcggatc gagggtgata
    11401 ttttcttttt ccacgccgga ctcctgtatt tataatttta attgtttacg taattgtgaa
    11461 ttacgcaagg ttgacattaa cggcaggtta aagttttagg actttactac cgctcacaaa
    11521 aacattttta gtcgactgat tgtgagcata ggtaagcggg aatttcccgg tatttatctt
    11581 ttgataaaac cgtgcacccg ctaatagttt gctgttttta atccgtactt aatgagcata
    11641 acgcgtgttc tcattaatgg atcgaatcat tgattgttta gctgaattca aatggttacg
    11701 gagcgcaagg gtggcatcaa ggtcactgcg gcaaatcaga gcgctgagaa tggtcatatg
    11761 ctcatcgacg gcaatgatat tgcgctgttt aagatcgctt tcgtcccatt gatagtgaaa
    11821 atgaaagatg actgagatga tctcaagcga ttgatcaaaa aagatattgt cggcagctga
    11881 aagcagtaag gagtgaaaat ctctgtcaag ttgcgagaac atgcgaaagc tattgccgat
    11941 gttgtcacgc agtagccgat gacgttcgag catggttttc gcctgcagcc agcggggatc
    12001 atgatcaggc aggttgagaa agtgctgaag tgaatgtgtt tcgagcattt cgcgtagctc
    12061 aaacagctgc tcggcgtagg attgatcgaa ttgcttcatg ctccactggc cgcgcttttc
    12121 gctgtgaata agattataac ggccgaattt taaaagatat tctcttacta caaccgggct
    12181 gacacccgcg gcccgagcca gttgcagttc agaaaacgtt tcccctgggc gtaactggcg
    12241 ctggtttatc atcgtgaaaa aagcctgctc aaacactttg ttttgttcac tcattgaagc
    12301 ggtagtacag gcgaaaccgt catcatggtc aggtttacgc gcaataacat agtcgtttcc
    12361 aacctgcgtc aagacgccgc attcgcgtaa gtggctgaga atatgacgca ctgtggtgcg
    12421 gctgatgttg tacatttccg ccagcgcact ttgcgatgga agtggggaag ggatatgacc
    12481 ccgcgccata tcatcaatga cctggttaat cacattgtgg cgtaaatttt gtgaacgact
    12541 catattttcc tccttgtgag tccattaaaa cccgatttaa aacattttta tgcgtgtgtt
    12601 ttcacaaaac agattttccg ttccgctgcc atttttcatt ttcattgata tctaaggcaa
    12661 caaaaaatgg agagccgaat catgtctacg atgaatgttt taatttgcca gcagccgaaa
    12721 gaattagtct ggaaacaacg cgagatacct attccgggtg acaatgaagc attaataaaa
    12781 attaagtctg tcgggatttg cggtaccgat attcatgcct ggggtggaaa tcaaccattt
    12841 tttagttatc cacgtgtttt aggccatgaa atatgtgggg agattgttgg gctgggtaaa
    12901 aatattgctg atcttaaaaa tggtcagcaa gttgctgtga t
//
LOCUS       DAZPLI010000117        12912 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZPLI010000117 DAZPLI010000000
VERSION     DAZPLI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12912
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.118"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            1..332
                     /locus_tag="ACHUU3_003093"
                     /pseudo
     CDS             1..332
                     /locus_tag="ACHUU3_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
     gene            655..2043
                     /gene="narU"
                     /locus_tag="ACHUU3_003094"
     CDS             655..2043
                     /gene="narU"
                     /locus_tag="ACHUU3_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR2083103.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2125..5865
                     /gene="narZ"
                     /locus_tag="ACHUU3_003095"
     CDS             2125..5865
                     /gene="narZ"
                     /locus_tag="ACHUU3_003095"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFR2083104.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKQLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5862..7406
                     /gene="narH"
                     /locus_tag="ACHUU3_003096"
     CDS             5862..7406
                     /gene="narH"
                     /locus_tag="ACHUU3_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR2083105.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7406..8101
                     /gene="narW"
                     /locus_tag="ACHUU3_003097"
     CDS             7406..8101
                     /gene="narW"
                     /locus_tag="ACHUU3_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR2083106.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            8098..8778
                     /gene="narI"
                     /locus_tag="ACHUU3_003098"
     CDS             8098..8778
                     /gene="narI"
                     /locus_tag="ACHUU3_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR2083107.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8857..9750
                     /locus_tag="ACHUU3_003099"
     CDS             8857..9750
                     /locus_tag="ACHUU3_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFR2083108.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9846..10691)
                     /gene="nhoA"
                     /locus_tag="ACHUU3_003100"
     CDS             complement(9846..10691)
                     /gene="nhoA"
                     /locus_tag="ACHUU3_003100"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFR2083109.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10864..11433
                     /locus_tag="ACHUU3_003101"
     CDS             10864..11433
                     /locus_tag="ACHUU3_003101"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFR2083110.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11437..11664)
                     /gene="pptA"
                     /locus_tag="ACHUU3_003102"
     CDS             complement(11437..11664)
                     /gene="pptA"
                     /locus_tag="ACHUU3_003102"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFR2083111.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(11764..12899)
                     /locus_tag="ACHUU3_003103"
                     /pseudo
     CDS             complement(11764..12899)
                     /locus_tag="ACHUU3_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3092 a         3292 c         3279 g         3249 t
ORIGIN      
        1 gctcatgtta tttaatttgt tttccccaac acttaagctt aatactggcc tggcaattct
       61 ttcgcctggt gcatttgaag ttcactctga cggaatagat gtggataacg aattgtttca
      121 ctatcctatt aaaaatagca tataccccat ataatataca cacttacaag acagaggaag
      181 ttgtaaacca gaggaatata aaagttaaaa acatgacctt agatgaaata aacaatactt
      241 actgtaataa cgattattac aatcaggcaa taagagagga accgatagac cttctggaca
      301 gatcgttttc ctccagttca tggccttttt agtgaataaa tcatcactaa ttctataagt
      361 gtctggtctt gttgataata tagttaatat gtaacccttt agattcacga tctgaagggt
      421 tgaaacatga catacgttca aagattaacc ccgtttgaag gttagcttgt tagttacatt
      481 tagtaacaca tatctggcta cttaatcccc attctgcccc cgcggttacg ctttcttatc
      541 tattctttca atcagcactt cacccaattc atccttaatt ctaataactg gctattgcgc
      601 cttacctcct gttttgtctt ttttcattac caatgtgtgc atgtgaggaa caatatggca
      661 ctgcaaaatg agaaaaatag tcgttatctt ttacgcgact ggaaaccaga aaatccggcc
      721 ttctgggaaa ataaaggaaa acatattgct cgaagaaacc tctggatatc agtcagttgt
      781 ctacttcttg ccttctgtgt ctggatgcta tttagcgcag ttaccgttaa tctcaataaa
      841 atcggtttta attttactac cgatcaactc tttttattaa ccgcattacc ctccgtttct
      901 ggcgcattat tgcgtgttcc ctactccttt atggtgccta tattcggtgg acgccgatgg
      961 acggttttta gtactgcaat cctgattatt ccttgcgtct ggctcggaat tgccgtgcaa
     1021 aatccgaata ctccttttgg gatatttatc gttatcgctt tgctatgcgg ttttgcaggt
     1081 gcaaactttg cttcgagcat gggcaatatc agtttcttct ttccaaaagc caaacaaggg
     1141 agcgctcttg ggattaatgg cggattagga aacttaggtg taagtgtaat gcagctggtt
     1201 gcaccgctgg tcatttttgt acctgtattt gcctttctcg gcgtcaatgg cgtaccgcag
     1261 gccgacggtt cggtgatgtc gctggcgaat gccgcatgga tttgggtacc gctactggcg
     1321 attgccacga tcgccgcctg gtcagggatg aatgatatcg ccagttcacg cgcctcaatt
     1381 gacgaccagc tccctgtctt acaacgcctg catctctggc tgctgagcct gctttacctt
     1441 gccaccttcg gttcgtttat cggtttttct gcgggttttg ccatgctggc aaaaacccag
     1501 ttcccggatg tgaatattct gcgcctggcg ttctttggcc catttatcgg tgccatcgcg
     1561 cggtcggttg gtggtgctat ttccgataaa ttcggcggcg tgcgggtgac gttgatcaac
     1621 tttattttta tggcgatttt cagtgccctg ctgttcctta ccttaccggg cacaggctcc
     1681 ggtaatttca tcgcctttta cgccgtattt atggggctgt ttctgactgc gggtctggga
     1741 agtggttcta ctttccagat gatcgccgtc atctttcgcc agataaccat ttatcgggtg
     1801 aagatgaaag gcggtagtga tgagcaagct cagaaagaag ccatcaccga aacggcggcg
     1861 gctctgggct ttatctcagc cattggcgca gtgggcggct tttttattcc gcaggcgttt
     1921 ggcatgtcgc tcaatatgac cggctctccg gtcggcgcga tgaaagtgtt tttaatcttc
     1981 tacatcgttt gtgtgctgct gacctggctg gtttatggtc ggcggaagtt cagccaaaaa
     2041 taagagatcg atgcctgatg tacaaccgtg tcgcatccga caaggcatca gatcccaatt
     2101 cattcctgga gcaggagtca tgtcatgagt aaacttttgg atcgctttcg ctacttcaaa
     2161 caaaagggcg aaacctttgc cgatggtcac ggacaggtga tgcatagcaa ccgcgactgg
     2221 gaggacagct atcgccagcg ttggcagttc gacaaaatcg tgcgttccac ccacggtgtt
     2281 aactgtacag gctcctgtag ctggaaaatc tacgttaaaa atggtctggt gacctgggaa
     2341 atccaacaga ccgactaccc gcgcactcgc cctgaccagc ccaatcatga acctcgcggc
     2401 tgcccgcgtg gcgcaagtta ctcctggtat ctctacagcg ctaaccgcct gaaatacccg
     2461 ctcattcgta aacaactgat tgaactgtgg cgtgaagctc tcaagcaaca cagcgatccg
     2521 gtactggcgt gggcatcgat tatgaacgat ccgcaaaagt gcttaagcta caaacaagtg
     2581 cgtgggcgcg gcgggtttat ccgctccaac tggcaggaac ttaaccaact gattgccgcc
     2641 gctaacgtct ggaccatcaa aatctatggt ccggatcgcg ttgccggttt ctcgccgatc
     2701 ccggcgatgt cgatggtgtc atacgctgcc ggaacgcgtt atctgtcgct gttaggcggc
     2761 acctgtttaa gcttctacga ctggtattgc gacctgccgc ccgcctcgcc gatgacctgg
     2821 ggcgaacaaa ccgacgtgcc ggaatctgcc gactggtata actccagtta catcatcgcc
     2881 tgggggtcta acgtaccgca gacacgtacg ccggacgccc acttctttac cgaagtacgt
     2941 tacaaaggca ccaaaactat cgccattacc cctgactact ctgaagtggc caaattgtgc
     3001 gaccagtggc tggcaccgaa acaaggcact gatagcgccc tggcgatggc aatgggccat
     3061 gtgattttaa aagagtttca tctcgataat cccagcgact actttatcaa ctactgccgc
     3121 cgctatagcg acatgccgat gctggtaatg ctggaacctc gcgacgatgg tagctacgta
     3181 cccgggcgca tgatccgcgc gtctgacctg gtagatggac taggcgaaag caataatccg
     3241 cagtggaaaa ccgtggcggt taataccgca ggtgaattgg tagtgccgaa tggttcgatt
     3301 ggtttccgct ggggagaaaa aggcaaatgg aatctggaat ccattgccgc cggtaaggaa
     3361 accgaattgt cgttaaccct gctcggtcaa catgacgctg ttgcaggcgt ggccttcccc
     3421 tactttggcg gcattgaaaa tccgcatttt cgcagcgtaa aacacaatcc ggtgctggtg
     3481 cgccaattgc ccgttaaaaa cctgacgtta gtcgatggca acacctgtcc ggtggtcagc
     3541 gtttatgatt tggtgctggc gaattacggc ctcgatcgcg ggctggaaga tgaaaacagc
     3601 gcgaaagatt acgctgaaat caaaccgtac accccagcct ggggtgagca aattaccggc
     3661 gtaccgcgcc agtatattga aaccatcgcc cgcgaatttg ccgataccgc ccataaaacg
     3721 catggacgct caatgattat cctcggcgct ggcgtcaacc actggtatca catggatatg
     3781 aactaccgtg ggatgatcaa tatgctgatc ttctgcggtt gtgtcgggca aagcggcggc
     3841 ggctgggcgc actatgtcgg tcaggaaaaa ctgcgcccac aaaccggctg gttgccgctg
     3901 gcctttgcgc tcgactggaa ccgcccaccg cgccagatga acagtacctc gtttttctac
     3961 aatcattcca gccaatggcg ctatgaaaaa gtctctgctc aggagttact ttcaccgctt
     4021 gccgatgcca gtaagtacag cggtcatctg attgatttca acgttcgcgc cgaacgcatg
     4081 ggctggctac cttctgcgcc gcagctgggg cgtaacccgc tcgggattaa agctgaagcc
     4141 gacaaggccg gattatcccc cacagaattt accgcccagg cgctgaaatc gggcgattta
     4201 cgtatggcct gcgaacaacc agatagcggc agcaatcatc cgcgtaattt gtttgtctgg
     4261 cgctctaacc tgcttggctc ctccggcaaa ggccacgagt atatgcagaa gtatctgctg
     4321 gggaccgaaa gcgggattca gggcgaggaa ctcggtgcca gcgacgggat caaaccggaa
     4381 gaagtcgagt ggcaaactgc ggcgattgaa ggcaagctcg acctgctggt gacgctcgac
     4441 ttccgcatgt ccagtacctg cctgttctcc gatatcgttc tgcccaccgc cacctggtac
     4501 gaaaaagacg atatgaacac ctcggatatg catccgttta ttcatccgct ttctgcggcg
     4561 gtcgatccgg cgtgggaatc acgcagcgac tgggaaatct acaaaggtat cgccaaagca
     4621 ttttcgcaag tgtgcgtggg tcatcttggc aaagaaaccg acgtggtatt acaaccccta
     4681 ctgcatgact ctccggcaga gctctcacag ccgtgtgaag tgctcgactg gcgcaaaggc
     4741 gaatgcgatc tgattccagg caaaaccgcg ccgaatattg tggcggtgga gcgcgactac
     4801 cctgctacgt atgaacgctt tacctcgctc gggccattga tggacaaact tggcaatggt
     4861 ggtaaaggga tttcgtggaa tacccaggat gaaatcgatt tcctcggcaa actcaattac
     4921 accaagcgtg atggcccggc acaggggcgt ccgctgattg acaccgccat tgacgcttca
     4981 gaagtgattc tggcactggc accggaaacc aacggtcatg ttgcagtcaa agcgtggcag
     5041 gcgctgggcg agatcaccgg gcgcgaacat acccatctgg cgctgcacaa agaggacgag
     5101 aagattcgct ttcgcgatat tcaggcgcag ccgcgtaaaa ttatctccag ccccacatgg
     5161 tccggtctgg aaagcgatca cgtctcctat aatgcgggat acaccaacgt tcatgagtta
     5221 attccgtggc gcacgctgtc gggacgccag cagctctatc aggatcatcc gtggatgcgt
     5281 gcttttggtg aaagcctggt ggcttatcgc ccgcctatcg acacccgtag cgtcagtgag
     5341 atgcgccaga tcccgccaaa cggcttcccg gaaaaagcac ttaacttcct gacgccgcac
     5401 cagaaatggg gcattcactc aacctacagt gaaaacctgc taatgctgac gctctctcgc
     5461 ggtggaccga ttgtctggat cagcgaaacc gatgcccgag aactgaccat tgtcgataac
     5521 gactgggtgg aagtctttaa cgccaacggc gcgctgacgg ctcgtgcggt ggtcagccaa
     5581 cgtgtaccgc cgggtatgac catgatgtat cacgcccagg aacgcattat gaatattcct
     5641 ggttcggaag taactggcat gcgcggcggc attcataact cggtcacccg tgtttgcccg
     5701 aaaccgacgc atatgattgg cggttacgcg cagctggcct ggggctttaa ctactacggc
     5761 accgtcggct cgaaccgcga cgaattcatc atgatccgca agatgaagaa cgttaactgg
     5821 ctggatgatg aaggtcgcga tcaggtacag gaggcgaaaa aatgaaaata cgttcgcaag
     5881 tcggcatggt gcttaacctc gacaaatgta tcggttgcca tacctgttcg gtgacctgta
     5941 aaaacgtctg gaccggacgc gaaggcatgg agtacgcatg gtttaacaac gtcgaaacca
     6001 aaccgggcat tggttatccg aaaaactggg aagatcagga agagtggcaa ggcggctggg
     6061 tccgcgatgt gaatggcaag atacgcccgc gtctgggcag caagatgggc gtaataacca
     6121 aaatcttcgc caacccggtg gtgccgcaga ttgatgatta ctacgaacct ttcaccttcg
     6181 actacgaaca tttgcatagc gcaccggaag gcaaacatat ccctactgct cgcccgcgtt
     6241 cgctgattga cggtaagcgg atggacaaag tgatctgggg gccaaactgg gaagaactgc
     6301 tgggcggcga gtttgaaaaa cgtgcccgcg accgcaactt cgaggccatg caaaaggaga
     6361 tgtacgggca gtttaaaaac accttcatga tgtacctgcc gcgcctgtgc gaacactgcc
     6421 tcaatcccag ttgcgtggcg acctgcccaa gcggcgctat ctacaaacgc gaagaagacg
     6481 gcattgtgct gattgatcag gataaatgcc gtggctggcg tttgtgcatt agcggttgtc
     6541 cgtacaaaaa aatctacttc aactggaaaa gcggcaagtc agaaaaatgt atcttctgtt
     6601 atccgcgaat tgagtcaggc caacccaccg tgtgctcaga aacctgcgtg ggtcgcatcc
     6661 ggtatctggg cgtgctgctt tacgacgccg accgcattga ggaagcggcg agcaccgagc
     6721 gcgaagttga cctctatgaa cgtcagtgcg aagtgttcct cgatccacac gatccctcag
     6781 tgatcgagga agccctgaaa caaggtattc cacaaaacgt gattgaagct gcccagcgtt
     6841 cgcctgtcta caaaatggcg atggactgga aactggcgct accgctgcac cctgaatatc
     6901 gcaccctgcc aatggtctgg tacgttcctc cgctgtcgcc gattcagtcc tacgcagatg
     6961 cgggcggttt gccgaaaagc gaaggcgtgc tgcccgccat cgaaagcctg cgtattccgg
     7021 tgcaatatct cgccaatatg ttgagtgccg gcgataccgg tccggtactg cgggcgctga
     7081 aacggatgat ggcgatgcgc cactatatgc gttcacaaac cgtggaaggc gttactgata
     7141 ctcgtgccat cgacgaagta ggcctgagcg tcgcccaggt cgaagaaatg tatcgctatc
     7201 tcgccattgc caactatgaa gatcgttttg ttatcccgac cagccatcgg gaaatggcgg
     7261 gcgatgcctt cgcagaacgc aacggctgcg gttttacctt tggcgacggt tgccacggtt
     7321 cggacagtaa attcaacctg ttcaacagta gccgtatcga tgccatcaac atcaccgaag
     7381 tgcgcgacaa agcggagggc gaataatgca gatcctcaaa gtgatcggcc tgttgatgga
     7441 gtatccggat gagctgttgt gggaatgcaa ggaggacgcg ctggcgttga tccgccgcga
     7501 cgcgccgatg cttacggatt tcacccacaa cctgcttaac gcgccgctgc tggataaaca
     7561 ggccgaatgg tgcgaagtgt ttgaccgcgg gcgcaccacg tcgctgctgc tgttcgaaca
     7621 tgttcatgcc gagtcccgcg atcgcggcca ggcaatggtg gacctgctgg cggagtatga
     7681 aaaggtcggc ctgcagctgg attgtcggga actgcccgat tatctaccgc tgtatctgga
     7741 gtatttaagc gtactgcctg acgatcaggc gaaagaagga ttgctcaatg ttgcgccgat
     7801 cctcgccctg cttggcggtc gcttaaaaca acgcgaggca ccgtggtacg cgttgtttga
     7861 tgctctgctg caactggcgg gaagtatact ttcaagtgac agtgtcacaa aacaagtaaa
     7921 cagcgaagag cgcgatgaca cccgccaggc gcttgatgca gtgtgggaag aggaacaggt
     7981 gaagtttatt gaagataacg ccacggcgtg tgacagctcg ccgcttaatc aatatcagcg
     8041 acgctttagc caggatgtcg cgccgcagta tgtcgacatc agtgcgggag gtgggaaatg
     8101 attcagtatc tgaacgtctt tttttacgat atctacccgt acatttgtgc gacggtgttt
     8161 ttcctcggca gctggctgcg ctacgactac gggcagtaca cctggcgcgc ctcctcaagt
     8221 caaatgctcg ataaacgcgg gatggtgata tggtcgaatc tgttccatat cggcattttg
     8281 gggattttct tcgggcactt gttcggcatg ttaacgccgc actggatgta cgcgtggttt
     8341 ttgccagtgg cagcgaaaca actcatggcg atggtgctcg gcggtatttg cggtgtgttg
     8401 acgttgattg gcggcgcagg actgctgtgg cgcagtctga caaatcagcg cgtgcgtgcc
     8461 acttccacta cgccggatat catcatcatg agcattttgc tgatccagtg tctgttgggg
     8521 ctaagtacca taccgttttc agcacagtat cctgatggta gcgaaatgat gaagctggtg
     8581 ggttgggcgc aaagcattgt cactttccgt ggtggatcgt cagagatgct caacggcgta
     8641 gcgttcgtct tccgcctgca tctggtgttg ggaatgacca tcttcctgct cttcccgttc
     8701 acccgactgg tgcatgtgtg gagcgcgccg tttgagtact ttactcgtcg atatcaaatt
     8761 gtgcgttcgc ggcgataatt tcaccctggc cggatagccc ttcgctctcc ggccacttgc
     8821 aggctatgat agttctccag tcacaggaga acgttaatga aaccgcaggt gtaccacgtc
     8881 gatgctttta cctctcaacc atttcgcggc aattctgccg gtgttgtttt ccccgccgat
     8941 aatcttagcg aagcacaaat gcagcttatc gcccgcgagt taggccattc ggaaactgct
     9001 tttctgctgc acagcgacga cagcgatgtg cgcatccgct actttacgcc aacggttgaa
     9061 gtgccgattt gcggtcacgc gacagtagct gcgcactatg tacgtgcgaa ggtgttaggt
     9121 ttaggaaatt gcacgatctg gcaaacatcg ctggcaggaa aacatcgcgt gactatcgaa
     9181 aagcacaacg atgattatcg tatttcgctg gaacaaggta cgccgggctt tgaactgccg
     9241 ctggagggtg aagtacgcgc ggcgattatc aacgcattgc atcttactga agacgatatt
     9301 ctgccgggtt tgccgatcca ggtcgcgacg accgggcatt ctaaagtgat gatcccattg
     9361 aagccggaag tggatatcga cgctctttcg cctgacctta atgcgctgac cgctatcagt
     9421 aaacagattg gctgcaatgg tttcttcccg ttccagatcc gtccaggcaa aaacgaaacc
     9481 gatggtcgta tgttctcgcc tgcgattggt attgtggaag atccggtgac tggcaatgcc
     9541 aatggcccga tgggtgcatg gttggtacat cacaacgtat tgccccacga tggcaacgtg
     9601 ttgtgtgtta aaggccatca ggggcgcgca ttggggcgtg acggcatgat tgaagtgacg
     9661 gtgacaattc gtgataacca accggaaaaa gtcaccattt caggcacagc ggtgattttg
     9721 tttcatgccg aatgggcaat tgaaatctga aatcgttgcc tgatgcgctt cgcctgtcag
     9781 gtctacaacc gggctaatgt atgccggata aggcgtttac gccgcatccg gcaacctgac
     9841 ataaattatt ttcccgcctc cggatgcgta tcaaacgccg ccatcaccag cgctaactca
     9901 tccacagtaa agccatgttt cgcatcatcc acgcccagac caaactgttc ctgcatcacg
     9961 gcatataacg acgccacatc cggtagattt cgctgctcca ccgcgtgccc attttcataa
    10021 tgggtaaaat gaaaattggt cagtgtcagc ttgccgccgt ccggcaaatg gcggcacatc
    10081 agcaaatgat ggcgaaaatg cgactgcggc cagtgcgccg accagaaatt gcccatcaca
    10141 taatcgcttt gttgctgctc gcagagatcg aagcggtaca tcggctgcca atgctgatga
    10201 tgattaaact gcaacaccca atcatcaccc tcctgcaaca accgatactc tccgtgtggc
    10261 gtggtctgca cgagatcgga aactaaacga atcggcgcgg ttagcgtctg cccaccgaaa
    10321 ccgacatcag caatccattt ttccccttcc agttccacca acagcaaacg atgggtgcgc
    10381 ggcggtaatg ctggcggatt tgataacact acgcgcccta acaagctgcg aacgttaaac
    10441 cccagctcgc gtaacacccg ctcaaacacg ccattctgct caaaacagta accgccacga
    10501 cgggctatca ccagtttctc ttccagcgat tgatcatcaa gctgtatttc cctcggcagc
    10561 aaaacgtcaa ggttttcaaa cggaatggtg caattgtgtt tcaggtgcaa tgcacgaagc
    10621 gtatcaatat tgaccgcagc agctcccgac cagttaatac gggcaaaata gtgattcaga
    10681 atgggcgtca tcaggcgttt cccttagtgg ttttctcaac ttataacctg gaatagacgc
    10741 cgcaccggct attttgtgcg atcgcggaca tgcatcacgt ttgcaaatcg ccaatcccgt
    10801 taatttcgtt acttattgcg ttacggacta cgcttaaaaa aaccttcttc gcgaggcgat
    10861 accatgagcc gattcatccc catagaatta caccatgcca gccgtctgtt aaatcatggc
    10921 ccaactgtct tgatcaccag tttcgatgag caatcccagc ggcgtaacat tatggctgca
    10981 gcctggtcaa tgccggtgga gtttgaacct ccacgcgtgg caattgtggt agataaatcg
    11041 acatggacca gggagttgat tgaacgtaac ggtaagtttg gcatcgttat cccgggcgtt
    11101 gcagcaacta actggacgtg ggcggtggga agtgtgtcgg tgcgtgaaga agataaattt
    11161 aattgctatg gcattccggt ggtgaaaggt ccggtgcttg gtttgccact tgttgaagag
    11221 aaatgcctgg cgtggatgga gtgccgcctg ctacctgcga cttctgcgca acaaaaatac
    11281 gacacgctgt ttggcgaggt ggtatcagca gcggcagacg cacgggtatt tgtcgaaggc
    11341 cgctggcagt ttgatgatga taagctcaat acgttgcatc atttaggtgc tggcacgttt
    11401 gttactagcg gcaagcgtgt tacggcaggt taagcattac atgctgtaac cgggtttctt
    11461 tatcaaagcc tccatttggg gctcgatttc ggcatcccag atagcttgcc aagattctgg
    11521 ttgaatctgc tgtagagcaa tgcttatcga gctatcttta ctgttcagat ggcgaataat
    11581 aacgtcggta atatctgcag caagtgctgc tttttgttgt tcgtccagtt cacgcggaaa
    11641 gcatttaatg tcgatgtgcg gcatgattac gctccttttg aaagatgtca gtaaaattaa
    11701 cacacattgt gaatgtcatg tgtccggtaa agcgtttata cgacaataat gcccagggca
    11761 agattacgaa agcccgctcc ccgtaaggac tgacgccagg tagtttctgc ccatggctgc
    11821 ttttcgcatc ttacgtctta accctgcctt gaatacctta tcattcgtca aaatattaat
    11881 agcaatgtgc cgtatccctg aaaataattc tgctgcattt cctcttctta ttttgcagtc
    11941 gtcttcattc attaccacgt ccagacgcca atgcagctta ttctccacgt gccagtggtt
    12001 tcggattgct gtggcgaact tctctgcggt taaatcagca gaactgatat aatatctgac
    12061 cgtcatttct ggctctttct tttgttctgc tattatggac cgaaaggaga ctgccacgca
    12121 taatttcttc agccctttcc attcaaacgt gaaatcaata agttcatcag ggacatcgca
    12181 aacaatatga agacggattt cttctctgcc gtgactcttt tcactaattg cgtaactgtc
    12241 attctctgga ttatttaatt ctttcagcgg aaatttttcc tcaaaggctt tatttagccg
    12301 cccctggttt ccttttaccg cgaataaata atcacctccc tgtttttgta tcttctctgc
    12361 aatatctttc tggcaaccca tcgcatcagt tgtgatgatt tttcctttaa tatccagcat
    12421 gttaagaagt tcaggaatgg ctgtaatctc attggatttc tcatccgtct taatctgccc
    12481 gagaatcaga ctgtgcattg ttgagaacgc actaatgaca tgaattgctc ccctgcggca
    12541 ctcttgtcat aagagtgccg gagcgttttt ccatcaattg caatgacgtc tttatcatct
    12601 gaagaatggc agtcacgcat ccagttaata aagcactcgt gaaattttgc aggactgata
    12661 caggatacaa ctctggcgat ggtatcgtga acaggaatac cattttcaaa atcaccatat
    12721 tgttttaaaa aatcgagatg tgtttcccca aaatcctcta tatcttccca gccttctgca
    12781 ccagaaataa cggcacaaat agtcaacagt agaatatccg ataatttatg ttccactttc
    12841 caggcttgtt tttaatcggg gataatagaa atatgttcca tcaatttttt aagttccatt
    12901 ttgttctcct ta
//
LOCUS       DAZPLI010000118        12563 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZPLI010000118 DAZPLI010000000
VERSION     DAZPLI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12563
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.119"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..833)
                     /gene="gap"
                     /locus_tag="ACHUU3_003104"
     CDS             complement(<1..833)
                     /gene="gap"
                     /locus_tag="ACHUU3_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR2083112.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IV"
     gene            1022..1552
                     /gene="cybB"
                     /locus_tag="ACHUU3_003105"
     CDS             1022..1552
                     /gene="cybB"
                     /locus_tag="ACHUU3_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR2083113.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1797..1970
                     /locus_tag="ACHUU3_003106"
     CDS             1797..1970
                     /locus_tag="ACHUU3_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083114.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            1996..2064
                     /gene="yncP"
                     /locus_tag="ACHUU3_003107"
     CDS             1996..2064
                     /gene="yncP"
                     /locus_tag="ACHUU3_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR2083115.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="ACHUU3_003108"
     CDS             complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="ACHUU3_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR2083116.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            2590..4230
                     /gene="trg"
                     /locus_tag="ACHUU3_003109"
     CDS             2590..4230
                     /gene="trg"
                     /locus_tag="ACHUU3_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR2083117.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(4268..5191)
                     /locus_tag="ACHUU3_003110"
     CDS             complement(4268..5191)
                     /locus_tag="ACHUU3_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR2083118.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            5408..6709
                     /locus_tag="ACHUU3_003111"
     CDS             5408..6709
                     /locus_tag="ACHUU3_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR2083119.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLY"
     gene            6976..8631
                     /gene="opgD"
                     /locus_tag="ACHUU3_003112"
                     /gene_synonym="mdoD"
     CDS             6976..8631
                     /gene="opgD"
                     /locus_tag="ACHUU3_003112"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR2083120.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            8771..8995
                     /locus_tag="ACHUU3_003113"
     CDS             8771..8995
                     /locus_tag="ACHUU3_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR2083121.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            9058..9594
                     /gene="rimL"
                     /locus_tag="ACHUU3_003114"
     CDS             9058..9594
                     /gene="rimL"
                     /locus_tag="ACHUU3_003114"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR2083122.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(9589..10569)
                     /gene="ydcK"
                     /locus_tag="ACHUU3_003115"
     CDS             complement(9589..10569)
                     /gene="ydcK"
                     /locus_tag="ACHUU3_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR2083123.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            10693..11685
                     /gene="tehA"
                     /locus_tag="ACHUU3_003116"
     CDS             10693..11685
                     /gene="tehA"
                     /locus_tag="ACHUU3_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR2083124.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            11682..12275
                     /gene="tehB"
                     /locus_tag="ACHUU3_003117"
     CDS             11682..12275
                     /gene="tehB"
                     /locus_tag="ACHUU3_003117"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR2083125.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3208 a         2927 c         3146 g         3282 t
ORIGIN      
        1 actatttctt catcggtata accaaatgac tcgttattgg tggttgcttg tttaagtgcg
       61 ttattcacct cttcggcagt cacttttttt ccgagaatcg ataccagttc agtgaccgaa
      121 cctgttttca ccggcacgcg ttgcgcatga cctttcagtt tgccgctcag ttccgggatc
      181 accagaccaa tggcttttgc cgcccccgta gtgtggggaa tgatattttc tgccgctgcg
      241 cgtgaagcac gtaaatcttt accacgtggg ccatccacca gtgactgggt gccagtatag
      301 gcatgaatgg tcgtcatcgt gccgacttct atcccgaaac tgtcatgcaa ggctttggcc
      361 atcggcgcaa gacagttagt ggtgcatgac gccacggaaa caatggtgtc gttgccatcc
      421 agagtgtcgt cattgacgtt ataaacgata gttttcattt caccggcagg ggcggaaatc
      481 aacaccttct tcgcaccagc atcaagatgt gcctgcgatt tctcggcgga ggtataaaag
      541 ccagtacatt cgacaatgat ttctgcacct ttcgctttcc acggaatatt tttagcctct
      601 ttttcggcgt aaaccgcgat acttttccca tcaacgataa gtgaatcttc cgtaaaatca
      661 acgctccagg ggaatggtcc gtagtttgaa tcatgtttca gcaggtaggc gagaattttt
      721 ggggaagtga gatcattaat agcgacaacg tctatgttgc ttttgacttc aagtaatcga
      781 cgcaacacca gtcgaccgat acgaccaaaa ccgttaatac caactttact catggttttc
      841 tcctgtcagg aacattcgga tgaaaattga tcctttccag gcttagacca ggattgtggg
      901 atgggcaatc tccattctta cagtgaaacg taacgtactg aaaacgggtg aacaatattt
      961 aatgaaattt tgagaaaagc ccgttatgtt aacggaaaat tatgttaaag caggaaatgt
     1021 tatggaaaat aaatactcaa ggttacaaat cagcattcac tggctggtct ttttgctggt
     1081 tatcgcagcg tattgcgcaa tggagtttcg tggtttcttc ccacgtagcg atcggccact
     1141 catcaacatg attcatgttt cctgcggcat ctcaattctc gtgctgatgg tcgttcgtct
     1201 tttgttaagg ctgaaatacc caaccccgcc gattatacct aagccaaaac cgatgatgac
     1261 gggactggcg catttgggac atttggtgat gtatctcctg tttattgcgc tgccagtgat
     1321 tggtttggtg atgatgtata accggggcaa cccgtggttt gcgtttggtt tgacgatgcc
     1381 ttacgcttca gaggccaatt tcgaacgggt tgatagctta aagtcgtggc atgagacgct
     1441 ggcgaatctg ggatattttg tcatcgggtt gcacgctgcg gctgcactgg cacaccacta
     1501 tttctggaag gacaacacac ttctacgcat gatgccgcgt aaacgttcct gaaggatatt
     1561 tcaagaaaat gcctgtacta aaaccgaccc gtggtacagg cgaagaatac gggtctacat
     1621 cggaagcgcc tattatattt atttgtatga caaataaaac cataatcctt gcccatacgt
     1681 ccatctggct tatttttaat caattcaccc gatctttgat ctcatcaacg gtatcaaaat
     1741 aaaccagcgt ataacgttca aatcgaacat aagattcgat aaaccatgga ggttatatga
     1801 aaaaactggc acttattttg tttatgggaa cgcttgtttc cttttatgcc gatgccgggc
     1861 gcaaaccctg ttctggttcg aaagggggga tctcacactg tacggcaggc ggcaaatttg
     1921 tctgtaatga tggttctatt agtgcatcga aaaaaacatg cactaactga agtgtaaaag
     1981 gggtgccatg agaaaatgaa tctgctagtc agatgcgcgg ggaaaatccc cgcgcttgcc
     2041 cttacctgga cgtgcaggcc atgagcgcag caacctcctt atcaccgtcc cggaaccgca
     2101 gttcgtagag cgtttgtcgg gtcaggagtg tgaatgtcag aatcgtaatg cagataatga
     2161 gcagacacac taccagaggg ttgtgcttca tagcctttct ccttgccgga tggcgggtaa
     2221 gaggctaaga tctgaattgc taggttcatt cgttggcctc ggttgataga aatatcggtc
     2281 ggggccttcg tctttctgat tcccggttag cctgaaaaca gaaagtctca ggcacccgca
     2341 ggcatcctat gaggtttcct tagggacgaa aataatcact tcacgaaatt gcgtgctgtt
     2401 ttccagaatt tttcgtcatt cgggttagcc agtttagcca ttcgttactc tcttcattcc
     2461 aatagcatta attttctctg caataattgt tgtaaaaatg tgacgcaaag aggtttttgg
     2521 tcataagtaa ttaccgtcaa gtgccgatga ctttctatca ggagtaaacc tggacgagag
     2581 acaacggtaa tgaatacaac tccctcacag cgattaggtt ttttgcatca catcaggctg
     2641 gttccgttat ttgcctgcat tcttggcggt atcttagttc tattcgcatt aagttctgct
     2701 ctggctggct atttcctctg gcaggccgat cgcgatcagc gtgatgttac tgcggagatt
     2761 gagattcgga ccgggttagc gaacagttca gattttttgc gttcagcccg gatcaatatg
     2821 attcaggccg gggctgcgag ttgtattgcg gaaatggaag caatgaagcg aaatattgcg
     2881 caagccgaat cggagattaa acagtcgcag caagggtatc gtgcttatca gaatcggccg
     2941 gtgaaaacac ctgctgatga agccctcgac actgaattaa atcaacgctt tcaggcttat
     3001 atcacgggta tgcaacctat gttgaaatat gccaaaaatg gcatgtttga agcgattatc
     3061 aatcatgaaa gtgagcagat ccgaccgctg gataatgctt ataccgatat tttgaacaaa
     3121 gccgttaaga tacgtagcac cagagccaac caactggcgg aactggccca tcagcgcacc
     3181 cgcttgggtg ggatgttcat gattggcgcg tttgtgcttg ccctggtcat gacgctgata
     3241 acatttatgg tgctacgtcg gatcgtcatt cgtccactgc aacatgccgc acaacggatt
     3301 gaaaaaatcg ctagtggcga tctgacgatg aaggatgaac cggcgggtcg taatgaaatc
     3361 ggtcgcttaa atcgtcattt acagcaaatg cagcattcac tggggatgac agtagggact
     3421 gttcgacagg gtgcggaaga gatttatcgt ggcaccagcg aaatttcagc tggcaatgcg
     3481 gacctgtcat ctcgcaccga agaacaagcg gcggctatcg aacaaactgc cgccagcatg
     3541 gagcaactca ctgcgacggt gaaacagaat gcggataacg cgcatcatgc cagcaaactg
     3601 gcgcaagagg cttctattaa agccagcgat ggcgggcaga cggtttccgg tgtagtaaaa
     3661 acgatgggcg ctatctccac gagttcgaag aaaatttctg agatcaccgc cgtcatcaac
     3721 agtattgctt tccagacgaa tattctggca ctgaatgctg ccgttgaagc cgcgcgagcg
     3781 ggtgagcaag ggcgtggatt tgccgttgtc gccagcgaag tacggacact cgcaagtcgc
     3841 agcgctcagg cggcgaaaga gattgaaggc ttgatcagtg aatcagtcag gttaattgac
     3901 ctggggtcgg atgaggtggc aacggccggg aaaaccatga gcactattgt tgatgccgtc
     3961 gcgagtgtca cacatatcat gcaggaaatc gccgccgcct cggatgaaca aagtagaggc
     4021 ataacgcagg ttagccaggc gatttctgaa atggataagg tgacgcaaca gaatgcttct
     4081 ctggtagaag aggcctcagc ggcggcggtg tcccttgaag aacaggcggc acgattaact
     4141 gaggcggtgg atgtattccg tctgcacaaa cattctgtgt cggcagaacc tcgcggagcg
     4201 ggtgaaccag ttagtttcgc tacggtgtga aaatgttcaa ggagggatcg acagatccct
     4261 tcacctttca gaacggcatt gattttcgaa tagcgttaat catcaactgg caaccagaag
     4321 agaacgtcgc atctacgcgg gtcagtattc caatcggttc gcctgcacca tgtcccggaa
     4381 caggcagggc caccagcgtg gcatgacgca ggtcgtcttt tacagcgcca gaagggacaa
     4441 accacacgta atcgtattca accgtaagtt gacgagatag cgaagcagac agcgtttcga
     4501 tacaacccga aggaatttta cagccctggc tctgcactaa tgcatctgaa tgctggcgtg
     4561 gcgcagtgcc ttctggtgat acaacgaccg gccattccag cacccggctt agcgttacgt
     4621 tctcctgaag tagcgggtga ttagggcgga caaccagctt cagcgattca agaaacagca
     4681 gttcgtaatt aagcccggtc atcagttcag gatctgacat ccgaccaatg ccgatatcga
     4741 tttccccggt ttttaaaccc gccagaatca tagggttact cattgtcgca acttgcaagg
     4801 tcgtctcttt ttgttgctga tgaaactgac ctataaccga aggtaatatc cccagtgccg
     4861 cagtaggtag tgcaccaacc ctgacgacat cattattaag accttcttta cgatgaagcg
     4921 actgtccggc agtgttgatg gcgtcaagaa ctctgactgc atgcgttaaa aattgttcgc
     4981 cgggtaaggt aagttgcgcc ccctgacgac cacgctcaaa caagcgagcg cccgtcagct
     5041 gctccagttc attcaatgtc ttagagagcg caggttgact caaattaagg gtttcagccg
     5101 cgcgccccaa agttccttgt tgtgcgacag ctacgaatgt atgaaggtgg cgcaaacgga
     5161 tgcgctgact aaacagacta tttttttcca taagcgatgt taaaaacgaa gcggtgtcgc
     5221 tgacaagtga agttgtttga ttatgataac ttgattgcaa aatattatta acaattaaag
     5281 caattatgtt acagcaaaat ggataatatt gatgttttcg cggcgagatc acagtttgta
     5341 aattcttccc gccagagtga atgcggttac ctacactcca gattactgac cactggaggc
     5401 agacactatg gcgaacagca tcacggcgga tgagattcgg gaacagtttt cgcaggcaat
     5461 gtcagccatg taccagcaag aagttccgca atatggcacg ctgctggaac tggtagctga
     5521 tgtgaatctg gctgtgctgg aaaacaatcc tcaactgcac gaaaaaatgg taaatgcaga
     5581 cgagctggcg cgactgaatg ttgaacgtca tggggcgatt cgcgttggga ctgcacaaga
     5641 gcttgctact cttcggcgga tgtttgccat tatggggatg tacccggtga gctattacga
     5701 tctctcgcag gcaggggtgc cggtacattc gacagcattt cggcccattg atgatgcttc
     5761 tctggcgcgt aatcccttcc gcgtttttac ctccttactc cgccttgagc ttatcgagaa
     5821 cgaaattttg cgccagaaag cggcggagat tctgcgtcag cgcgatatct tcaccccacg
     5881 ttgtcgacaa ctgttagagg aatatgagca gcagggcggt tttaacgaaa cacaggcaca
     5941 ggagtttgtg caggaagccc tggaaacgtt tcgctggcac cagtcagcaa cggtagatga
     6001 agaaacctat cgcgccttgc acaacgaaca tcggttgatt gctgatgtgg tctgttttcc
     6061 tggatgccat atcaaccacc tgacgccacg tacgctggat attgaccggg tgcagtcgat
     6121 gatgcctgaa tgcggaattg aacccaaaat tctcatcgag gggccgccgc gccgcgaggt
     6181 accgatttta ctacgccaga ccagctttaa agcactggaa gagacggtgt tgtttgcggg
     6241 gcagaaacag ggcacgcata ccgcgcgctt tggtgaaatt gagcagcgtg gcgtggcatt
     6301 aacgccgaaa gggcgacaac tgtatgatga tcttctgcgt aacgctggaa ccgggcagga
     6361 taatctcact caccaaatgc atttacagga aaccttccgc acttttcctg acagtgagtt
     6421 tttaatgcgt cagcaagggt tggcatggtt ccggtaccgt ctgacgcctt cgggtgaggc
     6481 gcatcgtcag gcgattcatc ctggagacga tccacagccc ttaattgaac gtggttgggt
     6541 agtggcgcaa cccatcacct atgaagattt cttgcccgtt agcgcggcgg ggatcttcca
     6601 gtcaaatctg ggtaatgaaa cgcagacacg cagtcacggt aatgccagtc gcgaagcatt
     6661 tgagcaggcg ttgggttgtc cggttttgga tgagttccag ctttactagg aagcggaaga
     6721 acgcagtaaa cgtcgctgtg gtttgcttta aattctgacc atccgccttt gcaaaaattt
     6781 gcctgatttt acaaacgaat caggctcatc ccatcgacat aaaaaaaatg ccgatttatg
     6841 catattctct cagttcaaca attggattat taataaatat tgtctagagt gagcggtcat
     6901 aaataagcac tttcttgccg ctgaaaacga ccagcgcggg accattcaca acaccagaag
     6961 gactcacttt caggtatgga tcgtagacga tttattaaag gttcaatggc tatggccgcc
     7021 gtgtgcggta ccagcggcat tgcttctctt ttttctcagg cggcattcgc ggcagattct
     7081 gatattgccg acgggcaaac ccagcgtttt gacttctcca ttctacagtc aatggcgcac
     7141 gacttagcgc aaacagcgtg gcgtggtgcg cctcgtccgt tacctgacac gctggcgaca
     7201 atgacgccgc aggcttataa cagtattcaa tacgacgccg aaaaatcgct ctggcataac
     7261 gttgagaacc gtcaactgga cgctcagttc ttccatatgg gaatgggatt ccgtcgccgc
     7321 gttcgtatgt tttctgtaga tccagcaaca catctggcgc gtgaaattca ctttcgcccg
     7381 gagttgttca aatacaacga tgcaggtgtt gataccaaac aattagaagg gcaaagcgat
     7441 ctcggctttg ccggttttcg cgtgtttaaa gcccccgaac tggcgcgccg tgatgtagta
     7501 tcatttctcg gcgcgagtta tttccgcgcc gttgatgata catatcaata cggtttgtcg
     7561 gcccgcggcc tggcgatcga cacttacacc gacagtaaag aagagttccc cgactttacc
     7621 gccttctggt ttgatacggt aaaaccgggg gcaactacct ttaccgttta tgcgttgctc
     7681 gatagcgcca gtattactgg tgcctataag ttcactatcc attgtgagaa aagtcaggtg
     7741 attatggatg tggaaaatca cctgtatgcg cgcaaagaca ttaaacagct gggcattgcg
     7801 ccgatgacca gtatgttcag ctgcggtact aatgaacgtc ggatgtgcga caccattcat
     7861 ccgcaaattc atgactctga tcgtctgtcc atgtggcggg gcaacggcga gtggatttgc
     7921 cgtccgctga acaatccgca aaaattgcag ttcaatgctt acaccgacaa caacccgaaa
     7981 gggtttggtt tattgcaact ggatcgtgac ttctcccatt atcaggacat tatgggctgg
     8041 tataacaaac gcccaagttt gtgggtggaa ccgcgtaaca agtggggtaa gggcaccatc
     8101 ggcctgatgg aaatcccaac aacgggcgaa acgctggata acattgtctg cttctggcag
     8161 ccagaaaaag ctgtaaaagc aggtgatgag tttgcattcc agtatcgtct gtactggagt
     8221 gcgcaaccgc ctgttcattg cccattagcg cgcgttatgg cgacgcgtac cggcatgggc
     8281 ggtttcccgg aaggttgggc gccaggtgaa cactatcccg aaaaatgggc gcgtcgtttt
     8341 gccgtcgatt tcgttggtgg tgatctgaaa gctgccgcgc caaaaggcat tgagccggtg
     8401 attacgcttt ccagtgggga agcgaagcaa atcgaaattc tctatattga acccatcgat
     8461 ggttatcgta ttcagtttga ctggtatccg acttcggact ctactgatcc ggtcgatatg
     8521 cggatgtatc tacgttgtca gggggacgct atcagtgaaa catggctgta tcagtatttc
     8581 ccgccagcgc cggataaacg tcagtatgtt gacgaccgcg tgatgagtta atcgtttttt
     8641 cttcggcacc ttcttcggga ggtgccgtct ggttaaacac gatcccgctc gcatttttcc
     8701 ctaagttaaa tgagtaatct gatggtgtgt atttcagata caccttgtca gccactaaca
     8761 gggagtgcgt atgtttccag aataccgaga tttaatatcc cgtctgaaaa acgaaaatcc
     8821 tcgctttatg tccttgttcg ataaacacaa taaacttgat catgaaattg ccagaaagga
     8881 aggttccgac ggtcgagggt acaatgcgga agtggtccgc atgaaaaaac aaaagctaca
     8941 gttaaaagat gagatgctca aaatcctgca gcaggagagc gtcaaagagg tgtaaacttt
     9001 cctaagccgc cagccaggcg gcttttttaa caacagcatg gattgactgg agataagatg
     9061 actgaaacga taaaagtaag cgaatcactt gaattacatg ctgttgcgga aagtcacgtc
     9121 acacctcttt atcagttaat ctgtaaaaat aaaacctggt tacagcagtc gctaaactgg
     9181 ccgcagttcg ttcagagtga agaggacacg cgaaaaacgg tgcagggtaa tgtgatgttg
     9241 catcaacgcg gctatgccaa aatgttcatg attttcaaag aagatgaact tatcggcgtt
     9301 atctcgttta atcgtattga accactgaat aaaactgctg aaatcggcta ctggctggac
     9361 gaatctcatc aggggcaggg gatcatttct caggcgctgc aggcattgat tcatcattac
     9421 gctcagtctg gtgaacttag acgcttcgtg atcaaatgtc gggtggacaa tccgcaaagc
     9481 aaccaggtcg ctttgcgcaa tggttttatc cttgaaggtt gcctgaaaca ggctgagttc
     9541 ctgaatgatg cctatgatga tgtgaactta tacgcgcgta ttatcgattc ataataaccc
     9601 tgccagcggc gttcgcgtaa tgcgctcctc gccgttaatg acttttggac cacggacatg
     9661 aaccgtgtcg ccatcaaagg cttcaacgac ggcgtggtcg gttaactcca catggttttc
     9721 aattatcacc gcacctgaaa tacggctctc accttgtatt acaacgtgct cgtcgagcag
     9781 aatcggtcca ccacgtacca cggcatttcc gccaatcaag acatgatgtt ttaacacaca
     9841 attaccttcc acaatggcat attccgccac ctgcgaactg taatgaatcg tcggaatggc
     9901 atcttcttct atgccagctt tcacctgcgc gtgaccgtag actttagcgc aatcgcatag
     9961 ccagacgttg ttctcttcat taccttcaac actggcaaaa tcaaaaactt cggcgcgatg
    10021 ttcaataaaa gcataccgga cgacggcatc gccataaatt tgtgcctggt ggacaatacg
    10081 cgaggcgcta acccttgcgc gatcataaat ttggagcagg agttgatggt cgggcgttaa
    10141 gccttgtgcg gcgacgatca tagaatgttg atcaattaag gcgtgaccaa atattctaca
    10201 ttgcccgtaa actaatgagt cacgaatggt gacactgtca cttatatatg caccctggct
    10261 gatttcacta ttatcgatcc agacgttatc cgttgcgtaa acctctcccc acagcacgct
    10321 ggtgccggtg atacgcgtat taccagaaat caccgttccg ccgaacgcaa tagcattctg
    10381 gtcgtaaatc cagcaatttc cttcctgtgc taacaccgtt tcgcgatcga tccagccgcc
    10441 agcggttcct gctatcacat cgttaaaatc gctaatggcg ataatctgcc gtaataacac
    10501 acttttttta gtgccatctt cctgataact aaaggctcgc tgttcttcac ttagacgata
    10561 tttgcgcata gcgttttctc acaggtgact tactataacc gtagcaaatt ctgcggctct
    10621 ggctatgctc gagaaaattc cataaaatgc atttcaaata tactttataa attaaacaaa
    10681 atgagtaaga agatgcagag cgataaagtg ctcaatttgc cggcaggcta ctttggtatt
    10741 gtgttgggga cgatagggat gggatttgcc tggcgctatg ccagccaggt ttggcaggtc
    10801 agccactggt taggggatgg gctggtgatt ctggcgatga tcatctgggg attattaact
    10861 agcgcattta ttacccgact catacgcttt ccgcatagcg tgctggcgga agttcgccat
    10921 ccagtgctga gcagttttgt gagtttgttt ccggcaacga cgatgctggt ggcgattggt
    10981 tttgttccgt ggtttcgccc actggcggtg tgcctgttca gttttggtgt cgtggttcag
    11041 ttggcttatg ccgcctggca aactgcggga ttatggcgcg gatctcaccc tgaagaagct
    11101 accacgcctg gactgtatct gccgacagtt gccaacaact ttatcagcgc aatggcctgt
    11161 ggtgcgttgg gctacaccga cgccggtctg gtgtttttag gcgcaggcgt tttctcatgg
    11221 ctaagtcttg aaccggtgat cttgcagcgt ctgcgcagtt cgggagaatt acccacggca
    11281 ctgcggacat cactcggcat tcagctcgct cctgcgctgg tggcctgtag tgcctggttg
    11341 agcgtcaacg gcggcgaggg tgatacgctg gcgaaaatgc tttttggtta tggactgctg
    11401 caactgctgt ttatgctacg tctgatgcca tggtatctct cccagccatt taatgcttca
    11461 ttctggagtt tctcgttcgg cgtatctgca ctggcaacca ccggtttgca tctggggagt
    11521 ggcagcgaaa atggattttt ccatacgctg gcggtaccgc tgtttatctt taccaatttt
    11581 attattgcaa tactgctcat ccgtactttt gcgcttctga tgcagggaaa attgttagtc
    11641 agaaccgagc gcgccgtttt aatgaaagca gaggacaaag aatgatcatt cgtgacgaaa
    11701 actattttac tgataaatat gaattaaccc gcacacactc tgaagtactg gaagcggtga
    11761 aagtggttaa accgggtaaa acgctggatc tgggctgtgg caatggtcgt aacagtcttt
    11821 acctggcagc caatggttat gatgttgacg catgggataa aaatgccatg agtatcgcca
    11881 atgtagagcg cattaaatcc attgaaaatc tggataattt acacacccga gtcgttgatc
    11941 tgaataacct cacatttgat agacagtacg attttattct ttcgactgtg gtactgatgt
    12001 tccttgaggc taaaaccatc ccagggctga ttgccaatat gcaacgttgc actaaacctg
    12061 gtggttacaa cctgattgtg gcggcgatgg ataccgctga ttatccatgt accgtcggct
    12121 tcccgtttgc cttcaaagag ggagaattac gtcgatatta cgaaggctgg gagagggtga
    12181 aatacaatga agacgtcggc gagctgcacc gcaccgacgc caacggtaat cgtattaaac
    12241 tgcgtttcgc cacgatgctg gcacgtaaaa aatgacccgc taagaataag gcgcgtgaaa
    12301 attccccacg ctgagatgat ttactgttct tcttttcggt aagcatattt tttatcgaag
    12361 ggatgtgaaa ttaatcacag tagtcgaagt tttttagcag cttaacttac tgaaatttaa
    12421 ctactgatga ttgacttagc ccctttttcg gcattgacta tgtcgtctga aaaggggctg
    12481 aaaaatttat tttcaccaac actttttttg ccacaacacg aagcggcgct ttttgctata
    12541 acttaaaaag taatataatc atc
//
LOCUS       DAZPLI010000119        12529 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZPLI010000119 DAZPLI010000000
VERSION     DAZPLI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12529
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.120"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(50..202)
                     /locus_tag="ACHUU3_003118"
     CDS             complement(50..202)
                     /locus_tag="ACHUU3_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR2083126.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(467..1201)
                     /gene="cysH"
                     /locus_tag="ACHUU3_003119"
     CDS             complement(467..1201)
                     /gene="cysH"
                     /locus_tag="ACHUU3_003119"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR2083127.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1276..2988)
                     /gene="cysI"
                     /locus_tag="ACHUU3_003120"
     CDS             complement(1276..2988)
                     /gene="cysI"
                     /locus_tag="ACHUU3_003120"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR2083128.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2988..4787)
                     /gene="cysJ"
                     /locus_tag="ACHUU3_003121"
     CDS             complement(2988..4787)
                     /gene="cysJ"
                     /locus_tag="ACHUU3_003121"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR2083129.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5106..5468
                     /gene="queD"
                     /locus_tag="ACHUU3_003122"
     CDS             5106..5468
                     /gene="queD"
                     /locus_tag="ACHUU3_003122"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR2083130.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5546..6817
                     /locus_tag="ACHUU3_003123"
     CDS             5546..6817
                     /locus_tag="ACHUU3_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR2083131.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6808..7068
                     /locus_tag="ACHUU3_003124"
     CDS             6808..7068
                     /locus_tag="ACHUU3_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR2083132.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7085..7660
                     /locus_tag="ACHUU3_003125"
     CDS             7085..7660
                     /locus_tag="ACHUU3_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFR2083133.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7808..8668)
                     /locus_tag="ACHUU3_003126"
     CDS             complement(7808..8668)
                     /locus_tag="ACHUU3_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR2083134.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8665..9444)
                     /locus_tag="ACHUU3_003127"
     CDS             complement(8665..9444)
                     /locus_tag="ACHUU3_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR2083135.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9422..10372)
                     /locus_tag="ACHUU3_003128"
                     /pseudo
     CDS             complement(9422..10372)
                     /locus_tag="ACHUU3_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10359..11792)
                     /locus_tag="ACHUU3_003129"
     CDS             complement(10359..11792)
                     /locus_tag="ACHUU3_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR2083136.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGINLAG"
     gene            complement(11862..12497)
                     /locus_tag="ACHUU3_003130"
                     /pseudo
     CDS             complement(11862..12497)
                     /locus_tag="ACHUU3_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
BASE COUNT         2868 a         3448 c         3159 g         3054 t
ORIGIN      
        1 gaccaaacca tcacaattca gtacgcgggg ttttaccccc gcgtggtggc tacttcttcg
       61 attcgtaagc gagaactgcc ttaaactcca taccacgttc tctgatactc aactcacaca
      121 acgagtcgcc taccatcagc gtgaatccca gtactgtaaa acacagcact atgattgcca
      181 caagggcata ttttgtcagc atgtcttgcc tccctgcaaa gaagagacta acattcgaat
      241 tgtttgggtt cgaacgctgg cctcaggttg atagaaatat cgcctggggc ttttgtccat
      301 ctggaacctc gcgaatgctt aacgccagac agcctcaagc acccgacgcc attctatacc
      361 tgataattct tccagcggcg ttcacgttta aaatcaggaa tactgctcgc aaaataaaaa
      421 cgcccccatc atttctgaca gaggcgttta atttatccgg caatatttac ccttcgtgca
      481 atccacattc cctttttaag ccaaagaaac gtgtttcttc ttccgacatg ccaggttccc
      541 atttacgggt tgtatgggta tcaccgaccg ataaatatcc ttcatcccat aatgggtgat
      601 atttcaggcc atgtttttgc aggtactgat aaatagttcg gttatcccag tcgataatcg
      661 gcagcacttt aaatacgcca cgctgaattg ccagcaccgg taaattggcg cgactgccgg
      721 attgttcacg gcgcaggcca gcaaaccagg tttgcgcgtt aagttctttc agagcccggt
      781 tcatcggttc gactttgttg atgtcattgt acttttcaat gccttcaacg ccctgctccc
      841 acagttttcc gtagcgtgct tcctgccagg ctgcgctttc ggtagcacgg tacactttca
      901 ggttgagctt gagtttgtcc gttaactcgt caataaagcg gtaggtttcc gggaacaagt
      961 aacccgtatc ggtgaggatc accggaatat ccgggcgaat ttgattcacc agatgcaggc
     1021 tcaccgccgc ctgaatgcca aagctggaag aaagtacata ttcaccgggc agattatcca
     1081 gcgcccaggc tacgcggcct tcagcgtcca gtttttccag ttcggcgtta gtttccgcca
     1141 gcgccagaat acgatctacc ttcggcagtt cgttcagggc gtttagatcg agtttggaca
     1201 tagattcctc actgtttgcc ttgcctgatg cgacgctcac gcgttcttat caggcctaca
     1261 agaccgggct gatggttaat cccacaaatc gcgcgccgga tcgagcaccg ggcgaatgat
     1321 gcccgcacgc accgtaaagt cgccgaagcc ttcacccgct tcgcgctctt tcgcccagcg
     1381 ccctatcagt tcatcaagcg acgccaggat ttccggctcg gtgatgtttt ctttatacat
     1441 ccgtgggata cgtgtcccaa tgcggttgcc gccaagatgc aggttgtagc gacccggcgc
     1501 tttacccacc aggcccactt ccgccagcat cgcgcgacca caaccgttcg ggcagcctgt
     1561 tacacgcatc acgatatgct catcgctgac accatgtttc gccattaaat tatcgatgtt
     1621 gtcgataaaa gacggcagga aacgctctgc ttccgccatc gccagcgggc aagtcgggaa
     1681 tgacacgcag gccatcgagt tttcacgctg cggcgtgacg gcattcatta acccgctctc
     1741 tttggcgatc ttctcgatct tcgctttctc gctttccggt acaccggtga tgatcagatt
     1801 ctggttcgcc gtaatgcgga aatcgccttt gtggatcttc gcgatctcca gcaggccggt
     1861 tttcagcgga cgccccggat aatcaaggat gcgaccattt tcgataaaca gcgtcaggtg
     1921 ccagttatca tcaatgccct taacccagcc aatacgatcg cctcgtccgg tgaactcata
     1981 tggacggatc ggttcaaatt tgatccccgc gcgacgctcc acttccgctt taaacgtctc
     2041 aacccccacg cgctccagcg tgtatttggt tttggcattt ttacgatcgg ttcggttacc
     2101 ccagtcacgc tgagttgtca cgacggcttc ggccaccgcc agcgtatgct ccagcggcag
     2161 atagccaaac tcgctcgccg tgcgggcgta ggttttcttg ttgccgtgtt cgatagaaag
     2221 cccaccgccc accagcaggt tgaagcctac cagcttgccg ttttcggcga ttgccacgaa
     2281 gttcatgtcg ttggcatgca gatcgatatc gttctgcggc gggatcacta ccgtggtttt
     2341 gaacttacgc ggcaggtagg tctgaccaag gatcggttct tcatcagtag tggcgacttt
     2401 ttcctggtcg agccagatct ccgcatacgc gcgggtacga ggcaacagat gctcagaaat
     2461 cttcttcgcc cactcgtacg cttccgcgtg cagctgcgac tcgtaagggt tcgaggtgca
     2521 gagtacgtta cggttcatgt cgttagctgt cgccagcgca tcaagaccga ccgagtgcag
     2581 catctggtgc accggtttga cgttcttttt cagaatgccg tggaactgaa acgtctggcg
     2641 gttggtcagg cgaatgctgc catagatggt gttttcaccg gcaaatttgt cgatcgcctg
     2701 ccactgttta gtggtaatca ccccacccgg cagacgacag cgaagcagca tcgcgtggcg
     2761 cggctccagc ttctgttcag cacgttcggc acggatgtcg cggtcatcct gctgatacat
     2821 gccgtggaag cggatcagca ggaagttgtc gcccttaaag ccgccggtca gaccatcgtt
     2881 taaatcttcc gcaatggtgc cgcgcaggta gttgctttca agcttcatgc gctcggcgtc
     2941 tgtcagtttt ccttcgacca ctaaaggccc tggatgtttt tcgctcatta gtagacatct
     3001 cgctgataac ggcgctctac gcgcagctca cttaaaaatt catccgccgc ttcggtgtcc
     3061 atgccaccaa attcggcaat cacttccaga agtgcctgct caacgtcttt cgccatgcga
     3121 ttagcgtcgc cgcagacata aatgtgggca ccgtcattga tccagcgcca cagctccgcg
     3181 ccctgttcgc gcagtttgtc ttgtacataa actttttctt tttggtcgcg cgaccaggca
     3241 agatcgatac gcgtcagcac gccctctttg acgtagcgct gccactccac ctggtagagg
     3301 aaatcttccg taaagtgcgg gttgccaaag aacagccagt ttttacccgg cgcttcgtcg
     3361 gcggcgcgtt gctgcataaa ggcacggaac ggcgcgatgc cggtaccggg gccaatcata
     3421 atcactggcg tttgcgggtt agcgggcagg cggaaattgt cgttatgttc gataaatacg
     3481 cggacttcgc cctcttcttc cacgcgatcc gcgaggaagc tggaggcacc accggcacgg
     3541 gcgcggcctt ccacgtcgta acgcaccaca ccaacggtga cgtgtacttc gttctcgact
     3601 tccgcctgcg aggaggcgat ggaatacagg cgcggggtca gcgggcgcag caggttaatc
     3661 agcgcttcgg catccagttg cgccggagag aaacgcacca tgtcgacaat cggcgtggtc
     3721 gcggcgtaat gctgtaactt cgctttatcg cccaccagcg gcagcagcgt ttcgctgcgg
     3781 gtaagcgtgg catagttctc aacaatgttg gcggtgttga cggtcagttc gaagtgccac
     3841 tgtagcgctt cgttcagagg caacgttttg ccctcgacgg tgacaggttc atcgcctttc
     3901 agccacagca gttcgacaag ttctttcacc agtgccgggt cgttctgata ccagacaccc
     3961 agcgcgtcac ccggctggta acgcaggcct gaatcgccta agtcaatttc gatatgacga
     4021 acgtcttttt cagagttacg cccggtaatt ttctggttaa ccgaaaggct cgccaccagc
     4081 ggcgcgtctt tgctgtacgg gctggtgtgg atttcattta ccgcgccagt agcgacggat
     4141 tgcgaaggtg ccgcgacagg cgcacgcgat ttaagcgcat caaccacgcg ggcgcgccac
     4201 tcgctggcag cagcctggta ttcaacatcg gcatcgacac ggtcgagcag gcgttcacca
     4261 cccagttccg ccagcttgct gtcgaaatct ttcccggact ggcagaaaaa ttcataagag
     4321 ctatcgccga ggctaaacac ggcaaacgcg gtgttttcca gctttggcgc ttttttggag
     4381 aacaggaact tatgcagcgc gacggcttct tccggcggtt ccccttcccc ttgcgttgac
     4441 gtcactacga tgagcagttt ttcgctggcg atttgtttga atttatagtc gcccgcgttc
     4501 accagcttaa cgttcagttt tgctgctaat aaatcatcgc gtaatgcttc agcaacccgg
     4561 cgcgcattgc cggtttgcga ggcggagata atagttatac ccggcatttc tgcggctggc
     4621 gctggcgtcg ctgcaagcgc agcaggctgc tgattgagta cgccccagaa atagccagaa
     4681 acccaggcaa gctgggtagg cgttaaatcg gtcgtggccg cctgaaggcg tgccagttgc
     4741 tccgggttca acggaagcaa cgcggaaggt gggacctgtg tcgtcatgcg tcgttatgtt
     4801 ccagtaagca aagctgtttc tgcgccctgt cagcgcccat aaaacagaag agaagataag
     4861 gttaacgggg tgaacggtgt ggattaaaga cgggatagcg ataactaata accaaatcga
     4921 ctaacctgtt ttagcaatag tctttaacaa caaaatagat taaccaaccc aatgaaaaac
     4981 aaatgaattt agccaatcat taagataaat cagcgatttt gcgcaacaaa gtcgttttag
     5041 ataatgcgaa aaaacagcct ttccggtact ctacggcggt tttatcgtcc tgtagagaaa
     5101 ttatgatgtc caccacgtta tttaaagatt tcaccttcga agccgctcac cgcttaccac
     5161 acgtcccgga agggcataaa tgtggtcgcc tgcacgggca ttcctttatg gtgcgactgg
     5221 aaattaccgg ggaagtcgat ccgcatacgg gctggattat cgatttcgct gaactgaaag
     5281 cggcgtttaa gccaacttat gagcgcctcg atcaccatta tctcaatgat attccaggtc
     5341 tggaaaaccc aaccagcgaa gttttagcaa aatggatttg ggatcaggtt aaacccgttg
     5401 tgccgctgtt aagtgcggtg atggtaaaag aaacctgcac cgcagggtgt atctatcgcg
     5461 gcgaatgata agagtgtgtc ggcggtcaat ttcccttaag taacgctatg ttagggtgtt
     5521 gtgttctgga tatctggggc atgacatgga agacgactgc gacattatta ttattggtgc
     5581 cggtattgca ggcaccgctt gcgcgttacg ctgcgcgcga gcgggtttat ccgttttgtt
     5641 actggaacgc gctgaaatcc ccggcagcaa aaatctttcc ggcgggcggt tatataccca
     5701 tgcactcgcg gaactcctcc cgcaatttca tctgaccgcg cctcttgaac gacgcatcac
     5761 tcacgaaagc ctttccctgt taacgcccga tggcgtaacg acgttttcca gcttacagcc
     5821 cggcggtgaa tcctggagtg tattacgtgc acggttcgat ccgtggctgg ttgccgaagc
     5881 cgaaaaagaa ggtgtcgaat gcatccccgg agcgacggtg gatgcactgt atgaagaaaa
     5941 cggcagagtc tgtggcgtta tttgtggtga cgatattctc cgcgcccgtt atgtggtgct
     6001 ggcagaaggt gccaacagcg tcctggctga acgtcacggg ttagtgactc gtcctgctgg
     6061 cgaagcgatg gcgttgggga tcaaagaagt gctgtcgctg gaaacatccg ctattgaaga
     6121 acgttttcat ctggagaata acgaaggcgc agcgttgctg ttcagcggcg gaatctgtga
     6181 tgacttaccc ggcggcgcat ttctttatac taatcaacaa acgctctcgt tagggattgt
     6241 ttgcccgctc tcttccctta cgcaaagtcg tgttccggca agcgagctgc tgactcgctt
     6301 taaagcgcat ccggcagtgc gcccgcttat caaaaacacg gaatcactgg agtatggtgc
     6361 gcatctggag ccagaaggtg gcttgcacag tatgccggtg caatacgccg gtaacggctg
     6421 gctgctggtg ggcgatgcgt tgcgcagttg cgtcaatacc ggaatttccg tgcgcggcat
     6481 ggatatggcg ctgactggcg cacaggcggc ggcacaaacg ctaataagcg cctgccagca
     6541 ccgcgagccg caaaatctgt ttgcgcttta tcatcacaac gtagagcgca gcctgctatg
     6601 ggatgttcta caacgttatc agcatgttcc ggcgcttttg caacgccctg gctggtatcg
     6661 ggcgtggcct gcgttaatgc aggatatttc ccgcgattta tgggatcagg gtgataaacc
     6721 tgttccaccg ctgcgccagt tattctggcg tcatttacgt cgtcatggcc tgtggcatct
     6781 ggcgggcgat gttatcagga gtctgcgatg tctgtagccc ataatctctg gcgcgttgct
     6841 gatgcgccgc acattgttcc ggctgactcc gttgagcgtc agatggtaca acggttgatt
     6901 aacgcctgtc cggcaggtct tttttcgctc acaccggaag gtgacttacg cgttgattat
     6961 cacggctgcc tggagtgtgg cacctgccgt ttgctgtgcg acgaatccac actacaacag
     7021 tggcgctatc cgccttccgg attcggcatc acctaccgct ttggataaat aataaggata
     7081 atttatgccc ctcttacacc tactccgcca gaatccggtg attgctgccg ttaaagataa
     7141 tgccagcctg caactggcta tcgattctga atgccagttt atttccgtgt tgtacggcaa
     7201 tatctgcacc atcagtaata tcgttaaaaa gattaagaac gccgggaaat atgcttttat
     7261 tcatgttgat ttgctggaag gcgcgtcaaa taaagaagtc gttattcagt ttttgaaact
     7321 ggtgaccgag gcggacggca ttatcagtac caaagcctca atgttgaaag cggcaagagc
     7381 ggaaggtttt ttctgtattc atcgcctgtt tattgttgat tcaatttcgt ttcacaacat
     7441 tgataagcaa gttgcgcaat cgaatccgga ttgtattgag atcctgccag gctgtatgcc
     7501 caaagtgctg ggctgggtga cagagaaaat ccgccaaccg ctgattgccg gtgggctggt
     7561 gtgcgatgaa gaagatgcgc gtaatgcgat taacgcgggt gtcgtggcgc tttccaccac
     7621 gaataccggg gtctggacgt tagcgaaaaa attactttga cgggataatc gtaaccaatt
     7681 gaatttggtt tgatttgtag gccgcacgcc acatccgaca ttcagcgcct gatgcgacgc
     7741 ttgacgcgtc ttatcaggcc tacaagtccc caggccagaa ccgtaggcag gatcatccgg
     7801 caacacgtta ttggcaatct gcgtgaatgt ttgtcaccag tgcctccagc accactttcc
     7861 agtcatcaac cacgccaaca tctgcctgtg aaaataccgc agcgctggcg tcatgattaa
     7921 tcgccaccac aaacttgcta ttgcgtacgc ccgccattaa cgctgctgcg ccagaagcgc
     7981 caacaacaat acacacctca ggtgccagca gatgtccgga aataccaatc actttttcag
     8041 catcaacgcc accgttcatc actcgcgccc gactgtagcc cacttcggct cccagctttt
     8101 ctgccagcat agcgatttcc tggttatctg cctctccgcc ctgccccacc accagcaccc
     8161 gtctggcctc agctaatggg tcgcgagtaa catttttcag atcctctgtg ctaaccagcc
     8221 agtccggaag cgcacccggg acaatgttca gttgttgcat tccagagggc aacgtggcgt
     8281 ttttcgctgc tcccgcctga cgcgccaggg aaaggcatag cgggcgtttt tctgtttgca
     8341 gcgtcgccgt tagcgcattt ccccagtggg atttgcgcac gcttacggtt ggaatatcta
     8401 acgaagtcac ctggcagatg cttgcaccat gtaaacgcca ggccagccgg gtcgacagtt
     8461 catcgccaaa cgttcccggc ggaaagagaa caacgtccgc aggcgttcgc tgccactgtt
     8521 ccaccaacgc atccagcacc tgttccgcca ctacaggctg aggttcaatc tgccagtgcg
     8581 ccagggtaca accgctgaaa tcctgcgccg ccagccagct tgctattgcg gcattctcct
     8641 gatttattgt cacaattgcg atattcatgg ctgcatcctt tggcgtaaat agtcctgcca
     8701 tagcttttga gctttttctg ctaccgtttg cccatcaatc agcgtcgctc cgcgtcgttg
     8761 ctctgctctt gccagttgca gacactgcat cgctggcatt tcggcagcaa cagttttacg
     8821 gataatttct gctttccccg cagccattcg ctggcgcatt cccggtactg gcaaggccac
     8881 ttcaccgcac tgacgcacgg caatcaccgc aggcaagcga acccggcaac aacgcaaccc
     8941 atgttcagta cgctgttcga gggtgataaa cagcgcgtcg agagtgaaac gctccacctg
     9001 ggtgaagcag ggccagccca gcatttccgc cagcaaaaat ggcgtttgcc cattctgccc
     9061 ttcgctgctt tggcagccgg taattatcag atccagcgga ttctgatgct gccattcggc
     9121 aatatggcga gcgacaaatt ccggcgcaaa gcgcagatct gccgccgtct ccagcaatac
     9181 agcttcctca aacccaagag ccatgagata gcgcagccag tgcaatgccc gttcatcccc
     9241 catgctcaac gcggttaaag acatcggcgt accgtttttt ctctgcgcca acagcagcgc
     9301 ggcagcggcc tgttcatcag caccgagtaa acttcgcagt agcgaaatat ccggtccgct
     9361 tttaccctga gccgccgcct gccactcttt ttccgccagc attccggcat ccggttcggc
     9421 tttaaacgct aacagaatgt tcattcgcac ctcctacatt tcccgccgcc accagcggga
     9481 gggctttagt ttctggtgcc catagccagg tcacaacaaa atcaaccaac aacactgtcg
     9541 ccaggagcaa taaggtgact tgcattcccc actgcgccag cacccacggc agcaggccag
     9601 tacttaccgc cgcgccaagt cgactcatgg cggtggcaaa accgacgccc agcgagcgaa
     9661 tgtcagtagg aaaactttcc gcaggcaaaa tgcccaccag attactgact gccgaaatgg
     9721 tggtgctgaa gagaacaaaa agcagcagcg ttaatgaact gccggaaggc aaacaggcca
     9781 tcactaccag cgttgccgcc agcagcaaaa aacttcccag caaaaatttg cgatgtgcca
     9841 gcaggtgcgt cagaactaat cccagcagcg cgcccacaat taacaacgca ttaagcatca
     9901 ggctggcagt cagcgcatct tccagaccaa tagtctgggc gatagttggc agccaggtat
     9961 aaatcacaaa ccatgggatt acgaggcaga caaagaagac gctgttaaac gccgtgcgcc
    10021 gccagtaacg cgaagagaac aaggttttga tgtgtttatg ggtcgccgtt accacttcat
    10081 cgcccagtaa aacatgggga ccaaaatagc gatgcacgat agcgtgagct tctgcaaaac
    10141 gcccctggcg cagtagccag cgtggtgatt ctggcgttcc ccagcgtaat aacgtaatca
    10201 acaacgcggg cagagctgcc gatgccagta gccagcgcca ggcctccggg ttttcggaaa
    10261 taaagtgatg tccggcaata cttgccagca catagcctac ggtccacacc acgctgaatg
    10321 cgcccagcaa aataccgcga tggcggcgcg gggaaaattc agccagcaag gttaatgcca
    10381 ttaggatcga actgcttttt cagcccttcc agcaacgccc acgcgctgcc gtgttccagt
    10441 ttgctccagt gaacgcgatg tttaccgata ccgtggtggt gcaccatcga accgccgagg
    10501 cgaatggttt cttcacagat gatcttgttg agcggattgt ggtacttgtc gatttcctct
    10561 tccggcttac agtcaacgac gttgtaatcg tagacgaagt acatgttggt gccgttctga
    10621 tagctatgag aggaatgacc gcccagcatg gtgatgtcgt cggcgtgcgg gaactcagta
    10681 cgaatacggt taataacgct ttcgtagatt tcgtggatgc agctccagca gccggacact
    10741 tcagtggtaa agcccatgtt gccggttttg aggatctgca cacgttcggc agccacttta
    10801 tccggtcccc agttcaggtt gttaaaccag gtttcgatca gcttgctgtc cacgcgctgg
    10861 cattgcgggt agcgggcaac gatttccgca atcccttcgc ccgtcgcctt cgcaatgcga
    10921 gggttacctt cagccataaa gatcagcacg cattttccgt ctgcaaaatg ggtgaagtgt
    10981 tgggtgccat cttcagcgtc atacaaacga gcgatcgacg gacgataccc ttccaccatg
    11041 acttcacgca ggatgttgaa gtcggttttc atgtcttcca ggatatagcc gtagaagagg
    11101 ttgttttccg gggtgaattt aaagattttc actgttactt cagtgatata gcaaagtgca
    11161 ccttcgttac cgatgatgat gtggcgaatg tccgggccag ccgcgcggcg tggcacgttt
    11221 ttaatgcgtg tgacggtgcc atcagccaat actgcttcca gaccaacgac catatcttcg
    11281 attgcgccgt agagtgtgga gaactgcccg atactgcggg ttgctaccaa gccgcccatc
    11341 tgcgccagcg gctttgactg cggagaatgc cccgtggtgt aacctttttc acgcaacgca
    11401 ttttccagca cctccagcgg aacgccacat tgcgccgtcg cctgcatatt ctcaatatca
    11461 atattaatga tttgattcat ggcggagccg tcaagcacca ccgagttttc cacaacggtt
    11521 tccagcccac cttcagtggc ggaagcgccg gtacgcggca caccgttaat tttgtgcgca
    11581 ttcataaaat tcagcacacg agatacttgc tctgtggaac cgagttttac gaccgctgcc
    11641 ggaatcggca aagtataaat gccatgaata tccggaaatt tacgaaaacg gtcaatactg
    11701 ttcttcttta atactgtttc atcggtaatt acgcgatctg caccaacaat ttcctttagt
    11761 tggtcgacaa tcgctgcgcg agataaagac atagtaattc cttcctgata ataaaaatgg
    11821 aataatcatt aataagagaa aacagaaacc accgttatgg attagcgcac taaataacca
    11881 ccattgacca ctaataaatg cccattgaca taatttgaag ccggacttgc gaggaataca
    11941 gctgcgccca ttaaatcctg agtatcgccc caacggtttg ccggaatatg atcaagaacg
    12001 cgctgattgg tttctggatt actgcgtgtc gccagcgtaa tatctgttgc ataatagcca
    12061 ggagcaatac catttacctg aatattatat tgacctagtt catcacaata agctttggtg
    12121 aatccggcaa gagcatgttt agtggcagaa tatgcaggtg accattgtcc acctaagtaa
    12181 gagaacaatg aacagatatt aataattttg ccgcttttct gcgggatcat aatttttgca
    12241 gcttcatagc ttaactcgaa tgcggcggtc aggttcacat caatcatcgg atcccagtcg
    12301 gcacgaccga agtccagcac cttattcagc ttacaaatac cggcattgtt taccagaata
    12361 tcaactgtac cgaaacgctc acagcaagca gcgataatct tctgcggcgc gccttctgcg
    12421 gtgatatcca cctgcatgaa gtccacctca acaccctgtt tttcaaacat ttcctttgtt
    12481 tcgccgttat ctttcaatca tttcctttgt ttcgccgtta tctttgacg
//
LOCUS       DAZPLI010000120        12223 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZPLI010000120 DAZPLI010000000
VERSION     DAZPLI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12223
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.121"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..510
                     /gene="csgD"
                     /locus_tag="ACHUU3_003131"
     CDS             <1..510
                     /gene="csgD"
                     /locus_tag="ACHUU3_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR2083137.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
     gene            515..904
                     /gene="csgE"
                     /locus_tag="ACHUU3_003132"
     CDS             515..904
                     /gene="csgE"
                     /locus_tag="ACHUU3_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR2083138.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            929..1345
                     /gene="csgF"
                     /locus_tag="ACHUU3_003133"
     CDS             929..1345
                     /gene="csgF"
                     /locus_tag="ACHUU3_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR2083139.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1372..2205
                     /gene="csgG"
                     /locus_tag="ACHUU3_003134"
     CDS             1372..2205
                     /gene="csgG"
                     /locus_tag="ACHUU3_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFR2083140.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPQAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2270..2761)
                     /locus_tag="ACHUU3_003135"
     CDS             complement(2270..2761)
                     /locus_tag="ACHUU3_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR2083141.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="ACHUU3_003136"
     CDS             complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="ACHUU3_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR2083142.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="ACHUU3_003137"
     CDS             complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="ACHUU3_003137"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR2083143.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="ACHUU3_003138"
     CDS             complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="ACHUU3_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFR2083144.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5405..5492
                     /locus_tag="ACHUU3_003139"
     tRNA            5405..5492
                     /locus_tag="ACHUU3_003139"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5439..5441,aa:Ser,seq:gga)
     gene            5537..5673
                     /locus_tag="ACHUU3_003140"
     ncRNA           5537..5673
                     /ncRNA_class="other"
                     /locus_tag="ACHUU3_003140"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5764..6450)
                     /locus_tag="ACHUU3_003141"
     CDS             complement(5764..6450)
                     /locus_tag="ACHUU3_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR2083145.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="ACHUU3_003142"
     CDS             complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="ACHUU3_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR2083146.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="ACHUU3_003143"
     CDS             complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="ACHUU3_003143"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR2083147.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="ACHUU3_003144"
     CDS             complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="ACHUU3_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR2083148.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10902..11732)
                     /gene="efeU"
                     /locus_tag="ACHUU3_003145"
     CDS             complement(10902..11732)
                     /gene="efeU"
                     /locus_tag="ACHUU3_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR2083149.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         3227 a         3167 c         2826 g         3003 t
ORIGIN      
        1 gacgatatat cttcaggctc tattattctt ctggatatga tggaagcgga taaaaagctt
       61 atccattatt ggcaggatac tttgagcagg aaaaacaaca atatcaaaat attgttgcta
      121 aatacgcctg aagattaccc gtaccgcgac attgaaaact ggcctcatat caacggcgtt
      181 ttttatgcca tggaggatca agaacgtgtt gtcaatgggt tgcaaggcgt cctgcgcggc
      241 gaatgctact ttacgcaaaa gcttgccagc tacttgatta cgcattcagg taactatcgt
      301 tataacagca cggaatcagc cctccttact catcgggaaa aagagatcct gaataaactg
      361 cgtatcggcg cgtctaataa cgagatcgct cgttcgttgt tcatcagcga aaatacggtt
      421 aaaacgcatc tttataatct tttcaagaag atagccgtaa aaaaccggac acaagcggtt
      481 tcctgggcaa acgataacct caggcgataa agccatgaaa cgttatttac gctggattgt
      541 ggcggcagaa tttctgttcg ccgcagggaa tcttcacgcc gttgaggtag aagtcccggg
      601 attgctaact gaccatactg tttcatctat tggccatgat ttttaccgag cctttagtga
      661 taaatgggaa agtgactata cgggtaactt aacgattaat gaaaggccca gtgcacgatg
      721 gggaagctgg atcactataa cggtcaatca ggacgttatt ttccagactt ttttatttcc
      781 gttgaaaaga gacttcgaga aaactgtcgt ctttgcactg attcaaactg aagaagcact
      841 aaatcgtcgc cagataaatc aggcgttatt aagtacgggc gatttggcgc atgatgaatt
      901 ctaaataaaa aattgttcgg aggctgcaat gcgtgtcaaa catgcagtag ttctactcat
      961 gcttatttcg ccattaagtt gggctggaac catgactttc cagttccgta atccaaactt
     1021 tggtggtaac ccaaataatg gcgctttttt attaaatagc gctcaggccc aaaactctta
     1081 taaagatccg agctataacg atgactttgg tattgaaaca ccctcagcgt tagataactt
     1141 tactcaggcc atccagtcac aaattttagg tgggctactg tcgaatatta ataccggtaa
     1201 accgggccgc atggtgacca acgattatat tgttgatatt gctaaccgcg atggtcaatt
     1261 gcagttgaac gtgacagatc gtaaaaccgg acaaacctcg accatccagg tttcgggttt
     1321 acaaaataac tcaaccgatt tttaagcccc agcttcataa ggaaaataat catgcagcgc
     1381 ttatttcttt tggttgccgt catgttactg agcggatgct taaccgcccc gcctaaagaa
     1441 gccgccagac cgacattaat gcctcaagct cagagctaca aagatttgac ccatctgcca
     1501 gcgccgacgg gtaagatctt tgtttcggta tacaacattc aggacgaaac cgggcaattt
     1561 aaaccctacc cggcaagtaa cttctccact gctgttccgc aaagcgccac ggcaatgctg
     1621 gtcacggcat tgaaagattc tcgctggttt ataccgctgg agcgtcagag tttacaaaac
     1681 ctgcttaacg agcgcaagat tattcgtgcg gcacaagaaa acggcacagt tgccattaat
     1741 aaccgaatcc cgctgcaatc tttaacggcg gcaaatatca tggttgaagg ttcgattatc
     1801 ggttatgaaa gcaacgttaa atcgggcggt gttggggcaa gatattttgg gatcggtgca
     1861 gacacgcaat accagctcga tcagattgcc gtgaacctgc gtgtcgtcaa tgtgagtacc
     1921 ggtgagatcc tctcctcggt gaacaccagt aagacgatac tttcctatga agttcaggcc
     1981 ggggttttcc gctttattga ctaccagcgc ttgctggaag gggaagtggg ttacacctca
     2041 aacgaacccg ttatgctgtg tctgatgtcg gctatcgaaa caggggtcat tttcctgatt
     2101 aatgatggta tcgaccgtgg tctgtgggat ttgcagaaca aagcagaacg gcagaatgac
     2161 attctggtta aataccgcca tatgtcggtt ccaccagaat cctgacagat aaaaatgccg
     2221 gaattaataa tccggcattt tactgtttaa gcgaaagcgt gagtgcccct cacgctttat
     2281 ttttgatttc ctgctcacgg tgagccgtct ttgcactacg cgccgccagc cacaggccgc
     2341 tgtttttcat tgcgtaacca aataccagcc caagcgccag cgaaggtaac accagtttcc
     2401 agtcaccctg cccggcaaac gtcgcgcatg cgcctataaa cgtccccgga acaaatgaaa
     2461 gcagcagctg tttggcctga atacacatca gaaacgccac aataccggtt atgacataac
     2521 cgagaatttc cagatgtggt gccagtgcac taccgtaaat aatgaccatc gcccacacca
     2581 cgccactaag cagggttgca gcggagatcg ccagcccttt cagcccacct tgcgggcagg
     2641 caaagtaggc cgtacagccc aagaagcccg cccagcttag taagcccagg gaaacagcca
     2701 cccatcccca gataccggag agaatgcctg ttgtgattgc aatagagaga agtatgttca
     2761 tgccgcgcat gatagcagaa aacgcggcca tgaatgagat cgagcacaca ttttatgcaa
     2821 caacaaatta tctgttgcag ttatgagtga cgatgatcac atttactctt cagaatcttc
     2881 ttccagctcg tcccacattg cactaatggc atcacgggtt agcggtgcca tggtgcgcca
     2941 gaaaggcgtg gttgcatggg cctccacttt gccgaggaac gcaccacacc agggtaacag
     3001 atactcactg aacagtgttt ccagtgcttc gctctcatct tccgttgact gatcttccag
     3061 ccaggaagcc gcaagcagca atgtgccgat gtgatcggct ggcgtatccg ccagtggcat
     3121 ccctcgttcg gaaagaaaag cgcgcacttc cgcttccgtt acgtcctcaa cccatgcgct
     3181 acgatatggc ggcactgcac attcatcgcc gataaacaac gcattgtaat cggcagagac
     3241 ttgcgccata tcacagcttt tctgtaaacg cgtcagtaac tcatcctgct ccagtggcca
     3301 gttcgcagcc agtttcccct cacgaatcag ggtaaacagc ggcaccagta aaggatcttg
     3361 cggttggcgg taatagagcg aacccagcac acgacagagg atagaaaact cgttcattaa
     3421 tatatttcca ttactagtaa ttaaaggtct gcaaattccg caatcggtgc cataccgcga
     3481 gattcaagga agttcagtaa gcggcgcgga gaaacattca aaatgcgctc tggcggaaaa
     3541 tctaccgcgt cgaggatttt aagacactct tcaaattccc ccatggtaaa cgcggtgtga
     3601 gaatccgagc ctaatgccac ccaacctcca gcatcacgta ccgctgcggc taccgcacga
     3661 cagttgtctt cactgccctt acgtgagtgt aaaaatgaag aattattgat ttccagcgcc
     3721 acctgatgtt tcgcggctgc ttcagcaacg gctttcacat ctatttcata tttgggattt
     3781 ccgggatggc ttattatatg cacattgccg ctggcgatag tggcgatcat cgcttgtgta
     3841 ttggtcgctt tgtcatgtgg tgcaaaaacc ggctcatgaa aaccggcaat aatcagatcc
     3901 agcgagtcaa acattttacc gctgcaatca atttcaccat caacattttt aatgttagct
     3961 tcgatgccgc gcaggatccc taccccatca accactcgcg gccagatacg catgttaatg
     4021 aagtgccagt gatgcggagc atcttccata tccgggccat gatcggtgat cgcaaaaagt
     4081 ttaatgccct tttgtttggc ctgggcaatg taatcactca atgtgctata tgcatgtgtg
     4141 ctggcaacgg tatgcatatg aaggtcgacg ggatacataa ctctctcctg tattcaattc
     4201 ccgccaagga tagcaggaat cctgatgctt tattagtagc cgcgtgcgcg gtcgacttgc
     4261 ccgcagaccc tctccccttt ttcgagctgg gcaatggtgc gagaaatgta ctccacagct
     4321 tcagcgggac gggtaatcgc ggcgacatgt ggtgttatcg tcacgcgtgg atgttgccag
     4381 agcggacttt caggcggtaa gggttcacga ttaaaaacat ccaacattgc gcctttaact
     4441 ttgccgctat ccagcgccgc gagcaggtca tcttccacaa catgaacacc acgcgccagg
     4501 ttgaggagat acgcgccatc cggtaatttt tcgagtaatt gttgattaat aatgccgacg
     4561 gtttcagggg tattcggtaa caaattaatc aatacccgac attggctcag aaatgcagac
     4621 agttcttccc gtccggcaaa gctttgcacg ccaggccacg atttacgggt tcgactccag
     4681 caacgcagcg gaaagcgcca ggtttgcaga ctctgagcaa ctttactgcc cagtacgcct
     4741 gcgcccaaaa tgccgatggt aaaatcttcc cgatgatatt caggcagcgg ttgccaatgc
     4801 gaactatttt gctggatgcg ataatcgtca aaacgtcgaa accaatgcag cacctgactg
     4861 acagcatatt cctgcatttg ctcgcccata ccggtatctt ccaggcgaaa aagtggaaca
     4921 gaagggttca gcatttcagg gtgtgcctgt agcttgctca aaatagaatc aacaccggcc
     4981 ccgagtgcga acaccgcttt aagatcgcgc cctgccagca tttcaacagg aggatgccag
     5041 actaaagcat aatcagcaga gtcattatct ccgcttttcc atgctctgac tcttgcctga
     5101 ggaatagctt tgcgcagtgc ctcaatccac cattgggtat cgaacgttgg gtgataaaag
     5161 atgatatcca tactgactcc cgaaaagcgt tgtgcgaaat ttattcgcac ttatcgttat
     5221 gatctacaaa ggccaccagc ataacaaatc cgtggtcggt ggcaaaaaaa gcagatttcg
     5281 cttattaaaa ccacacattg attgaagttt gaataaacgc gcgatttttt caaaaagttt
     5341 gttgacctca ggtcatgatt tccctaaatt agcgcccgtt ccagcaagac aggaacgaca
     5401 atttggtgag gtgtccgagt ggctgaagga gcacgcctgg aaagtgtgta tacggcaacg
     5461 tatcgggggt tcgaatcccc ccctcaccgc catatttaaa gaagagctcg tacgaaagta
     5521 cgggcttttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccggggttcg
     5581 acaactggcg acagccagtt ggacagaccg tgaacgcagt gagcgggctg cccgcagggc
     5641 gagcgaagcg agtcaatccc cccctcaccg ccatctttaa agaagagctc gtacgaaggt
     5701 acgggctttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccgggatact
     5761 tagttaatgc accggttcac tctttgtcgc cgcgactaat gggcgaattt tgtcacgagt
     5821 ctcccagacc ggaggaattt cctccaattt agtgacagaa gctcgtacaa aaggcacccg
     5881 tgcaagagtc ataattgtat ccaaccaacg gtcacgaata atgcgatccg ccctttcatg
     5941 actcgggtca tccagctcaa taacacagac tattttattg ttcttctttg aaaccagaac
     6001 ataatccatt gccatgccat tataaattgc aaagaaacgc tttctttctt cttcgctaaa
     6061 ctcaggttgt tctggaaagt tgatcaagcg ccccagagag acctggcaat ggatatgaca
     6121 ataatgtcca aaccattttt ccagattatc gaaatacatt ttttcacaac gcgtgattaa
     6181 ccactctttt ctttccaata aaataccttc ggcaaccatt ccctgaaata actcatctcg
     6241 ctcttgccaa cgagagtcac gataggtttt ctgcggtata tagctccttg tcgacgcctt
     6301 agcggcaggg gaagttgcag atgaagggac gggttgcgat ttagcgatga ttaccgatga
     6361 ggcaactttt cggagtgtca attttctggc aacagcaaaa ccggcgataa atgcaattgt
     6421 caaacaagtt acaaaagcca taaagctcat aattaatttt cacctatcca tgttgattat
     6481 atttcattac caatgttact cgctgcagcg tgttacatgc caacaatgca acgatcaata
     6541 tatagttagt atcatgtttt gattattaca accatcccta tttagccatt gcggcttgcc
     6601 ttccacaaaa aaataacaac acacaatatt agcaaagaat taattaagat taaccttaca
     6661 tttataatat tatccctaaa gaataaatca gaggaatata ttcggtgtta aacagtcagt
     6721 caaagtatag cccattgttg attaaacctc ataagcaaaa ttggcaccag atctcctgta
     6781 caggttgaat ggccgaaaaa agataggcag atgctcattt ttttggcttc tggaagaaaa
     6841 actctccgcg ctggcagtat tacactggtc tgatgctatg acgaaaaatg cctggtaaga
     6901 gtatctaaac cagactccag ttcagcttat caacacgctc aaagtcgccc ccctgaacgc
     6961 taacctgaag tgaaaatctg ttgaaataaa acaaagcccg gttcgcccgg gctctgcacc
     7021 gataacacac ttaactgtag gcatgcagcg tacgttggca aagtgccgaa cgtacgcagt
     7081 cctctttacc gaaccggacg atcccaacca tttcatcttc ttcgaaacgt tccagcgcgt
     7141 cacttaatcc ggagcacacg ccgcgaggca aatcgcattg cgtgatatca ccgttgacga
     7201 taaccgtcac gttctccccg aggcgggtta aaaacatttt catttgcgcg gcagtcacat
     7261 tctgcgcctc gtcaagaatg acgactgcat tttcaaaggt acgtccacgc atataggcga
     7321 acggcgcaat ttccaccttc cctatttccg gtcgcaggca gtactgcata aaggaagccc
     7381 ctaagcgccg gaccagcacg tcgtagaccg ggcgaaaata gggagcaaac ttttctgcga
     7441 tatctccagg taagaagcca agatcttcat cggcttgcag aactggacgg gtgacgataa
     7501 tcctgtcgac atccttatgt atcagggcct ctgccgcttt tgctgcgctg atccaggttt
     7561 ttccgccccc ggcttcgccc gtggcgaata tcagctgctt actctcaata gccttcagat
     7621 agtgcaattg cgcttcattt cgcgcgagga tgggcgaagt atcgcgactg tcgcgggcca
     7681 taccaatggc ttctacgccg cccatctgca caagcgaggt gaccgattct tcttcacgct
     7741 gcttatggct gcgcgaatcc cgtctcagca cacgttttgc ctcgcgacga gctttgatca
     7801 ctgctttttg tcttcccata gagagcacct tgagttgttt gtattcatca cacgcgccgt
     7861 tggcagcgcg attatgcgca cgaacatcag agggttggct tccttgtaag ccatagtttg
     7921 cttttggata aaatgccgaa aaacggctac gcgcaccgtt tacggcgtcg gtaacacatg
     7981 aaaagaaagg atgaggttga aaatgcaaag tgacgagatg actaccggag gagaaaactc
     8041 cgcgagtggt ggcgcgttga ttatctaaaa catgtccagt acaggacgtt accatccgcg
     8101 atctccatag tgactgacta tcactgccgg gaacttccgc tgctacttaa taagtacaac
     8161 agatctcgca tttattgcaa caatatattt acttatattt aactataaaa cactatttca
     8221 gtgacattag tttctactgg aaagatgaca gagtgatgac agtgatgaaa aaagctgtgt
     8281 gctttcagca ggatttgcat caggcatcag tgcacgatcg ccggatgcgg cgtgaacgcc
     8341 ttatccagcc taccaaaccc gcactgattg taggcatgat aagacgcgca agcgtcgcat
     8401 caggcatctg cgcacaaatg ccttatccgc ctaaaaacat taaacccgca ataacgcgct
     8461 tccgagataa tcgttcgcgt ccttcactcc cggcagcgaa aaaaaataac cgccgccgat
     8521 aggtttaacg tattcctcca gcgcttcgcc attgagcctt ttttgtactg tcaggaagcc
     8581 tttttccaga tcgtgttggt agcagacaaa cagcaacccc atatccagtt gtccggagtt
     8641 ggtgaccccc aacgaataac tgtagccgcg acgcagcatc aggctggact cactctccgc
     8701 cgtgcgggga ttcgccagcc ggatatggct gtccagcgca atcaccttcc cttccgggtc
     8761 gctggcgtaa tcaggcacat catgctcatg ctgcattccc agcggcgcac cggtttgctt
     8821 atcacggcca aaaatcgtct gctgttcttt cagcggcgtt ctgtcccaaa attccactcg
     8881 aaactgaatc aagcgtactg cctgatagct gccaccgatt gtccacgcag gctcctgctg
     8941 atctgccgtt acccacacca ctttttgcat caacttatca ttctggctat cgggattggc
     9001 agtgccgtct ttgaaaccca gcaaattaat cggcgtctct ttgcctttac tacgcgacac
     9061 gtgatcggaa ataaaccctt cccgcttcca gcgcacactg agcaaatccg gcgtgtgttt
     9121 gatgatatcg cgcagcgcat ggataaccgt gtcctgggtg ttggcgcaaa tctgtagcaa
     9181 cacatcacca tgacataacg ccgcatccag cgagtcgttg gggaaacgcg tcatcttctg
     9241 cagctttttt ggcatctgtg gcgcaaggcc aaagcgctca tcaaacaatg agtgacccac
     9301 cgataacgtg atggtgagat tatcgggcgc aatgtagccg ccaagaatgc cggaatcgag
     9361 tggtggcagg cgcggatttg gcgtttctgg tgctgctccg ccctgagtca gaaaagcaaa
     9421 acgctgagtc aacaagcgaa acaaccgctc aagatcggct ttatcgctgg caagcacatc
     9481 aaacgccacc agcatcattg cggcctgttg tggcgtcagg atccctgcct gatgctcacc
     9541 ataaaacggc tgtttctcat tgcgtgcatc cggtgaaagc gtgcccggtg cactttgcgt
     9601 tttttgtgca tgagcgaccg gacaacttcc cgccagcgcc agcgcaccaa tccctttcag
     9661 taaacgtcgg cgtgacggtt cattcacgcc gttttcatct ttatactgca taacgcttaa
     9721 tccagtccca gcacaccgcg aagttgcgcc agatcttccg ccagcgcagt aatcggtcct
     9781 ttcagtgcat tccggtcggc atcggtcaat ttgtcgtagg tttcaaaacc gtctttagta
     9841 cggtatttcg ccagaatggt atcgaccttt ttaaagttgg catcgacttt tgccagcagt
     9901 tccgggttgg ctttttgcag ttgtggacgc agcaaatcga caattttctg cgagccttca
     9961 acgttagcct ggaaatccca cagatcggtg tggctgtagc gatcttcttc accgctaatt
    10021 ttgctggctg ccacttcctc aatcagtccg gctgcgccgc cgaccacttt tgaaggtggg
    10081 aaagccagtt cactgatgcg tttttgcaaa tcgaccacat ctgtataaag ctgctcagcg
    10141 tactgatcca tccctttggt ggtgttgtcg ccaaacaatg ctttttccag acggtggaaa
    10201 ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat cttcacgggc gtcaatgctg
    10261 ccatccagat cggagaacag ttcagcaatc ggttcaatgc gctcatagtg ctggcgcgtc
    10321 ggcgcataca gtgctttcgc tttttcgata tcgcctgctt taatcgcgtc ggtaaaggct
    10381 ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat acgctttata tgcagtaatt
    10441 gcaccaccaa gacttaacag cgcatcactt tgcgccgcat ccgccgttgc ctcacctttg
    10501 acgatcaact tccctttcgg gttagtcagc agaccgcagg tcatatcgta ttcgccaggc
    10561 tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga tattttcccg ctcttccacc
    10621 accatcacgc ctttgaggat ctcccactcc agcgccttct ggctatggtt ctgaataatg
    10681 aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt cgcactgctt atccgtcacg
    10741 gtcactttga cctgcggcac atcagcggcg ttagccataa aagcagaaga aaacagcgca
    10801 gccacgctca actgcaatgc gttacggcgg aagttaatgg tcatgacgag actatccctt
    10861 taaagtatgt tgtaactaag caagagtttg cgtcgtattt gttacgcgga gcgagacgct
    10921 gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg ccgggatgag ataaataaac
    10981 cagacggcga cttcgctgac gctcggtgct tcctgatagc caaaaatccc ttccatcagc
    11041 gtgccaaaca gcgagtgagt tgagagcact gcgctcatat cgaaggcgat ttcctgaaag
    11101 tggttccaca atccggcttc atgaaatgcg cgaatggcac cagcagccag ccctgcggcg
    11161 acgaagagaa taaacaggct ggtccattta aaaaatgcac caagattgag gcgaataccg
    11221 ccccagtaga gcaggaagcc tagcaccacg gcagtagcaa gaccgagcat tgcacccagc
    11281 ggcggccaga tcccgacatc ttgttgaaat gccgccagca ggaaaaagac cgactccagc
    11341 ccttcccttg caacggcaaa aaagaccatc atcaccagcg cccagccatg atgatttcca
    11401 cgctgcaatg cgctatcgac tgcctgctcc agttgtactt tgacgttgcg cgacactttg
    11461 cgcatccaga aaaccatcca ggtaaggatc accacggcga tcaccgccac gataccttca
    11521 aacagttcct gttctttttg cggaaattcg ccggtggttt cgttaatgaa gatacccaag
    11581 cccaggcaca acgcagcggc aagcaacacg ccaatccaca tcacgccaat ccatcggcct
    11641 cgctgggtac gcttaagata gctggcaatc aaactgacaa tcagcgcggc ttcaagtcct
    11701 tcgcgcaaca taatgagaaa cggaacaaac atgccaccca cccttaaacg tttttctgag
    11761 tcaatccatg caaagaaaag taaatcgcag tgatagcgat tatcattacg gcaaagaaaa
    11821 atacaagcgg aatgtttata aaatgatgtt ggaatgtaaa caaagagagg taataccagg
    11881 ggatataaac gattattcca ggtacaaaga tgggattgtt tacaacgagc catgtgttct
    11941 tgccagatgc cgcgtaaacg ccttatccag ccgacaaaac tgtccagact caatgcatca
    12001 cagcttccat gtaaaactga taagcgggtg tatcaagcaa tttcgtccat ccgcgattat
    12061 caaatctaac tttttgcccg gaacataata acagcgcggg tgttggtggc gtcaaaatcg
    12121 ctgaaacgtt ccccgaaggc cggggcagcc cacatggatg tgggctgagg gcgcgtctta
    12181 caggacgtta cctcgcgccc gacccggtag ccgaagggga taa
//
LOCUS       DAZPLI010000121        12080 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZPLI010000121 DAZPLI010000000
VERSION     DAZPLI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12080
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.122"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHUU3_003146"
     CDS             complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHUU3_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFR2083150.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPL
                     FLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAICQ
                     SPEAHPDKDQRWLAAAVAGIFYLLAGLFG"
     gene            1006..1542
                     /gene="sutR"
                     /locus_tag="ACHUU3_003147"
     CDS             1006..1542
                     /gene="sutR"
                     /locus_tag="ACHUU3_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFR2083151.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHUU3_003148"
     CDS             1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHUU3_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFR2083152.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3668..3898)
                     /locus_tag="ACHUU3_003149"
     CDS             complement(3668..3898)
                     /locus_tag="ACHUU3_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFR2083153.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4120..4274
                     /locus_tag="ACHUU3_003150"
                     /pseudo
     CDS             4120..4274
                     /locus_tag="ACHUU3_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            4320..4736
                     /gene="hicB"
                     /locus_tag="ACHUU3_003151"
     CDS             4320..4736
                     /gene="hicB"
                     /locus_tag="ACHUU3_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFR2083154.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            4815..6221
                     /locus_tag="ACHUU3_003152"
                     /pseudo
     CDS             4815..6221
                     /locus_tag="ACHUU3_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            6466..7611
                     /gene="ydcS"
                     /locus_tag="ACHUU3_003153"
     CDS             6466..7611
                     /gene="ydcS"
                     /locus_tag="ACHUU3_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFR2083155.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7629..8642
                     /locus_tag="ACHUU3_003154"
     CDS             7629..8642
                     /locus_tag="ACHUU3_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR2083156.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            8643..9543
                     /locus_tag="ACHUU3_003155"
                     /pseudo
     CDS             8643..9543
                     /locus_tag="ACHUU3_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            9533..10327
                     /locus_tag="ACHUU3_003156"
     CDS             9533..10327
                     /locus_tag="ACHUU3_003156"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR2083157.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10349..11773
                     /gene="patD"
                     /locus_tag="ACHUU3_003157"
     CDS             10349..11773
                     /gene="patD"
                     /locus_tag="ACHUU3_003157"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR2083158.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            11983..>12080
                     /locus_tag="ACHUU3_003158"
     CDS             11983..>12080
                     /locus_tag="ACHUU3_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HFR2083159.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNK"
BASE COUNT         2946 a         2914 c         3223 g         2997 t
ORIGIN      
        1 ccaaacagac ctgcgagcaa atagaaaatg cctgcaacgg cagctgccag ccaacgttga
       61 tctttatccg gatgcgcttc cgggctttgg caaatagccg cggtgattgc cgcaataccg
      121 acggaataaa cgccgaaagg ggaaaaaacc agtgccagca atccagtaaa tacaattaat
      181 ggcgaaacag gagccgaata tccagctgct ttcattgctg cgatacccgg tgcgttttgc
      241 gatgccatcg tcaccagaaa aagggggagt gcaacgctca ggctgtgagc aaacgaaaaa
      301 tcaggggtaa tataagtggg gagaacgggt ttaaagacaa catcagttgt gacaacgtca
      361 ccttgcgcga taacgatcac gatcccaata atcatcgcgg caattaccgc atagcgcggc
      421 gcaacggcct tggttgccag ccataccagc aacatacttc cacacaacgt aaattgaccg
      481 tccagactgg caaacgcctg taaaccaaag cgtaataaaa tcccggcaag cattgccgcc
      541 gcaagcgagt gcggaataat gcgcatcaga cgagcaaaga gtcccgttat gccgcagagg
      601 actattaacg cgttggtgac aataaaaacg ccgatggctt cgttaagtgt tagtccctgc
      661 aatccggtga ccaacaaagc cgcgccaggc gttgaccatg cggtgagaac aggtacgcga
      721 taccataatg tcagagtcag cgtactgacg cccattgcca gccccagcgc cgtcatccag
      781 ccagagattt gtgcagtggt ggctccggcg acaatcgctg cttgccagat tattgccgct
      841 gaactggcgt agccaattaa taccgccaga aaccccgcca gtagcgtggg tggaggaata
      901 gaaaacagac gcatagtcac ctcgtgcgtt atagcgtaca ggcaccgtag catttgtccg
      961 ttataacgca caagtgataa acttccgttt tgccggagga gtcgcatgga aaatctcgct
     1021 cgctttttat ccactacact taaacaacta cgccagcagc gcggctggag tctttcgcga
     1081 ctggcagaag cgacgggcgt ttctaaagca atgttagggc agattgagcg taatgagtcc
     1141 agtccgacgg tcgcgacatt atggaaaatt gccaccgggt tgaacgtacc attttcaaca
     1201 tttatttctc cgccgcagtc cgctatgcct tcagtttatg atccacaaca gcaggcgatg
     1261 gtgatcactt cgctgtttcc ttatgatcca caactctgct ttgaacactt ctcgattcag
     1321 atggcatctg gtgcaataag cgaatcgacg ccccatgaga agggggtaat cgaacatgtc
     1381 gtcgtcattg atggacaact tgatctgtgc gttgatgggg aatggcagac ccttaattgt
     1441 ggggaaggcg ttcgatttgc tgcagacgtc acgcatatct atcgtaatgg tggggagcaa
     1501 accgtacatt ttcattccct catccattac ccgcgcagtt aagcgggaaa actatttcgc
     1561 aacgtcgcgc ttgtggctaa aatagccgcc atttttcagc tactggataa gaatgtgacc
     1621 gtatcttctc atcgacttga actgttaagc ccggcacgcg atgccgccat tgcccgcgaa
     1681 gctattttgc acggtgccga tgctgtttat atcggcggcc ctggttttgg tgcccgtcat
     1741 aatgccagta atagcctgaa agatattgcc gagctggtgc cgtttgccca tcgttatggt
     1801 gcaaaaattt tcgtcacgct taacaccatt ttgcatgatg atgagctgga acccgcgcaa
     1861 cggctgatta ctgacctcta ccagaccggt gtcgatgcgc tgattgttca ggatatgggg
     1921 attctggaac ttgatattcc gccgattgaa ctgcacgcca gtacgcagtg cgacattcgt
     1981 acagttgaaa aagcgaagtt cctctctgat gttggcttca cgcagattgt gctggcgcga
     2041 gagctgaatc ttgatcagat ccgcgcgatt caccaggcta cggacgcgac cattgaattc
     2101 tttattcatg gggcactgtg cgtggcctat tcgggtcagt gctacatttc tcatgcgcaa
     2161 acagggcgta gcgccaaccg tggcgattgc tcgcaggcgt gccgtttgcc atacacattg
     2221 aaagacgatc aggggcgggt ggtttcctat gaaaaacatc tgctgtcgat gaaagataac
     2281 gatcagactg ctaacctcgg cgcgctgatt gatgctggcg tacgctcctt caagattgaa
     2341 gggcgttaca aagatatgag ctacgtgaag aatatcaccg cccattatcg ccagatgctg
     2401 gatgccatta ttgaagaacg tggcgatctg acgcgtgctt catcaggtcg tactgaacat
     2461 ttctttgttc catcgacgga aaagactttc caccgtggta gcacagatta ttttgtgaat
     2521 gcccgtaaag gcgatattgg cgcgttcgat tcgccgaaat ttatcggcct gccggtgggt
     2581 gaagtattga aagtggcgaa agatcatctc aatgttgccg tcaccgagcc actggcaaat
     2641 ggtgatggcc tgaacgtgat gattaaacgt gaagtcatcg gttttcgtgc caatacggtc
     2701 gagaaaaccg gagaaaatca gtaccgcgtc tggcccaatg aaatgccagc agatttgcac
     2761 aaaattcgtc cacatcaccc actaaaccgt aatcttgatc ataactggca gcaggcactg
     2821 acaaaaacct ctagcgaacg tcgggtggcg gtagacattg aactgggcgg ctggcaggaa
     2881 caactgattc tgaccctcac cagtgaagag ggtgtcagca tcacgcatac gctggacggg
     2941 cagttcgacg aagccaataa cgccgaaaaa gcaatgaaca atctgaagga tggtctggca
     3001 aaactggggc aaaccctcta ttacgcccgc gatgtgcaaa ttaatttgcc gggggcgctg
     3061 tttgtaccaa acagtctgtt aaaccagttc cgccgtgaag ctgctgacat gctggatgct
     3121 gcgcgtcttg ccagttacca gcgcggcagc cgtaaaccgg ttgctgatcc tgcgccggtt
     3181 tttccgcaaa cgcatctgag tttcctcgcg aacgtataca accagaaagc gcgtgaattt
     3241 tatcatcgct atggtgtgca gctgattgac gcggcgtatg aggcacatga agagaagggc
     3301 gaagtcccgg tgatgatcac caagcattgt ctgcgctttg cctttaatct gtgcccgaaa
     3361 caggcgaaag gcaatatcaa aagctggaag gcgacgccaa tgcaactggt taacggcgat
     3421 gaagtattaa cgctaaagtt tgattgccgc ccatgcgaga tgcacgtcat tggcaaaatc
     3481 aaaaatcaca tactgaaaat gccgttaccg ggaagcgtag tggcatccgt aagtccggat
     3541 gagctgctga aaacattgcc taagcgaaaa gggtaaaacg ccagttttct ggttactcac
     3601 aactcattga atctgcatga tattgcctgc cgggtaaggc gtcacgccgc atccggcatc
     3661 aaacgactca acgttgtccc gcttctggtt tgcgcgattt ttgccagtaa aaatgctcgc
     3721 gcaaaccttc cgccgactct tccgccacag cacgcaattc atcgctgtcc gcttcatgac
     3781 gcagctgatg atccacattc tttacccaca caaattcatg tcctttgtgc cctgccatga
     3841 gttgtcctga gaacagcgca cacgttaata agacaaccga taacgccttc gtaaacatcc
     3901 tgccaccttt ttcttacctt ttgccgctat gatgccgatc gtttcttgag gttattattc
     3961 agttttgcaa attagcgcaa agaaattctg gaatcttcct tcctgatttt gcattgcatt
     4021 ctgccgttgc ggcgatttag tgctattttc gagcaaatta cacatagggg taaacactaa
     4081 tgtttacttt attgatatac tcagcggcag ggaggcgatg tgaaacaaag cgagttcaga
     4141 cgttggctcg aatctcaggg cgtcgatgta gcgaatggcc gcaaccattt gaaactcagg
     4201 tttcatggga ggcgcagtgt catgccgcgt cactcctgcg taaagcaatc ctgaaacaac
     4261 tcggtttgag ttaatcgcca attaaaaagg ttaatgacat gcgagagaca gtcgaaatta
     4321 tgcgttatcc cgtcactctt acacccgcgc cggaaggcgg ttatatggtt tcttttgtgg
     4381 atatccctga agcgttgacg cagggcgaaa ctgtcgctga agcgatggaa gcggcaaaag
     4441 atgctttact gaccgcattt gatttttatt ttgaagataa cgagcttatc ccgttacctt
     4501 cgccattaaa tagtcatgat cactttattg aagtaccttt gagcgtcgcc tctaaagtat
     4561 tgctgttaaa tgctttttta cagtcagaaa tcactcagca agagttagcc aggcgaattg
     4621 gcaaacctaa acaggagatt actcgcctat ttaacttgca ccatgcgaca aaaatcgacg
     4681 ccgtccagct tgcggcaaag gcgcttggca aagagttatc gctggtgatg gtttaattac
     4741 agttaacgaa aagttgtcat ttttaacaac tgatatagac tgccgaatca tctgcacata
     4801 attacgattc gataatgaaa aaataccagc agcttgcaga acaattacgc gagcagattg
     4861 cgtcgggtat ctggcaaccc ggcgatcgtt tgccttcgtt gcgtgaccag gtggcgcttt
     4921 caggcatgag ctttatgact gtcagccatg cctatcagtt gctcgaaagt cagggatata
     4981 ttatcgcacg accgcagtcg ggttattacg ttgcgccaca ggcaataaaa atgccgaaag
     5041 cgccagtcat tccagtcact tgagatgaag cagtcgatat caacacttat atttttgata
     5101 tgttgcaggc cagtcgcgaa ccgtcggtcg ttccgtttgc ctcggccttt cccgacccgc
     5161 gacttttccc cctccaacaa ctaaaccgct cgctggcgca ggtaagcaaa accgccacag
     5221 cgatgagcgt gattgaaaac ttaccgccag gaaacgcaga actgcgtcag gctattgctc
     5281 gtcgctatgc cttacagggc atcaccattt ctcctgatga aattgtcatt actgccgggg
     5341 cgttagaggc attaaacctc agtttgcaag cggtaactga accgggcgat tgggtgatag
     5401 tagagaatcc ttgtttctac ggtgcgttgc aggcgctgga gcggctacgg ctgaaggcgt
     5461 tatcggtggc gacggatgtt aaagaaggga tcgatcttca ggcgctggaa ctggcgttgc
     5521 aggattatcc ggtgaaagcg tgctggctga tgactaatag ccagaaccca ctcggattta
     5581 ccttaacgcc gcaaaaaaaa gcacaactgg tggcgttact caatcagtac aacgtaacgc
     5641 tgattgaaga tgacgtttac agcgaacttt attttggacg ggaaaaaccg ctgcctgcga
     5701 aagcgtggga tcgccacgat ggcgttttgc attgctcttc gttttcgaaa tgtctggtgc
     5761 ctggttttcg tattggttgg gtcgccgccg gaaaacatgc acgtaaaatt caacgcttgc
     5821 agttgatgag tacgctttcc accagctcac cgatgcaact tgcgctggtg gattaccttt
     5881 ccacgcgccg atacgacgcc catcttcgtc gcctgcgtcg ccagcttgcg gaacgtaaac
     5941 aacgtgcctg gcaggcactg ctgcgttatc tgcctgcgga agttaaaatt catcatagtg
     6001 acagcggtta ttttctctgg ctggagctcc ccgagccgtt agatgccggc gaattaagcc
     6061 tggcggcact gacgcatcat atcagtattg cgccgggtaa aatgttttct accggtgaaa
     6121 actggtcacg tttttttcgt tttaataccg cgtggcagtg gggagaacgt gaagaacagg
     6181 cggtaaaaca attaggcaaa cttattcaag aacggctgta atagcgttta atttaattcc
     6241 tcttagattg ggtaatatga atttcgaata gcagtcatat ttcctaaccc cttgtctata
     6301 ctccagaaga taaccttaca gacggcataa tgcgcggtag ctcacaacct gattaaattt
     6361 tctcagggac gaaggtgtgc ctgcaagccg ccgtctatgg ttaaacaagg agatattttt
     6421 acggcacggc ggctgaacaa ttaattacga caggagtaag accttatgag caagacattt
     6481 gcccgcagca gcctgtgtgc gctcaccatg acaataatga ccgctcacgc cgccgaacca
     6541 cctaccaatt tagataaacc ggaagggcga ctggatatta tcgcctggcc gggatacatc
     6601 gaacgcggac aaactgataa acaatacgac tgggtaacgc aatttgaaaa agagacaggc
     6661 tgcgcggtga atgtgaaaac cgccgcgact tccgatgaaa tggtcagtct gatgaccaaa
     6721 gggggttacg atctggttac ggcatccggc gatgcctcgc tgcgtttgat tatgggcaaa
     6781 cgcgtgcagc cgattaatac cgccttgatt cccaactgga aaacgctcga tccgcgcgtg
     6841 gttaaaggcg actggtttaa tgttggcggc aaagtttacg gcacacctta ccaatggggg
     6901 ccgaacctgc tgatgtacaa cactaaaacc ttcccgacgc cgccggatag ctggcaagtg
     6961 gtttttgttg agcaaaatct gccggacggc aagagcaata aaggccgcgt tcaggcttat
     7021 gatggcccta tctacattgc ggacgctgcg ttgttcgtta aagccactca gccgcagttg
     7081 ggcatcagcg atccgtatca actaaccgaa gaacagtacc aggcggtgct gaaagtgctg
     7141 cgcgctcaac acagtttgat ccatcgctac tggcatgaca ctaccgtgca aatgagcgat
     7201 ttcaaaaacg agggtgtggt tgcttccagt gcctggccct atcaggccaa cgccctgaaa
     7261 gccgaaggcc agcctgttgc taccgttttc ccgaaggagg gtgttaccgg ttgggctgat
     7321 accaccatgc tgcatagcga agcgaaacat ccggtttgcg cctacaaatg gatgaactgg
     7381 tcattaacgc caaaagtgca gggcgatgtg gcggcctggt ttggctcgtt accggtagtg
     7441 ccggaagggt gtaaagccag tccgttatta ggcgaaaaag gttgtgaaac caacggtttt
     7501 aactatttcg acaaaatcgc cttctggaaa acgcctatag cagaaggggg caagtttgtt
     7561 ccctacagtc gctggacgca ggattacatt gccattatgg gcggtcgcta acttcgctgg
     7621 ggtgctttat gacgtacgca gtggagtttg acaacgtctc gcggttgtac ggtgacgtgc
     7681 gcgcagtaga tggcgtcagt attgcgataa aagatggtga gttcttctct atgctggggc
     7741 cgtccggctc cggcaaaacc acctgcctgc gcctgattgc tggcttcgaa cagctttccg
     7801 gcggggctat ctctatcttt ggtaaacccg ccagcaatct gccaccgtgg gagcgggacg
     7861 tgaatactgt ctttcaggac tacgcgctat ttccgcatat gtcgattctt gacaatgtcg
     7921 cctatgggct gatggtcaaa ggcgtgaata aaaagcagcg gcacgcaatg gcgcaagagg
     7981 cgctggagaa agtggcgttg gggtttgtac agcaacgtaa accgtcacaa ctttctggtg
     8041 gtcagcgcca gcgggttgct atcgccagag cattggtgaa tgaaccgcgc gtattgctgt
     8101 tggatgaacc gctcggcgca ctggatctca aattgcgtga gcagatgcag ctggaactga
     8161 aaaaactgca acagtctctc ggtatcactt ttatcttcgt tacccacgat cagggcgaag
     8221 cgttatcgat gtccgatcgt gtggcggttt tcaataatgg acgcattgag caggtcgatt
     8281 ccccgcgcga tctctatatg cgcccgcgca cgccgtttgt tgccgggttc gttggtacat
     8341 cgaatgtttt tgatggactg atggcagaga aactttgtgg catgacggga agcttcgccc
     8401 tgcgaccgga acatatccgc ctcaacaccc ctggtgaact gcaggccaat ggcacgatcc
     8461 aggcggtgca atatcagggc gcggcaactc gttttgaact gaaattgaac ggcggtgaaa
     8521 aactgcttgt gagtcaggcc aatatgacag gcgaagaact gcctgccacg ctcacgcccg
     8581 gacaacaggt gatggtttcc tggtcgcgtg atgtgatggt gccgctggtt gaggagaggt
     8641 gaatggcgat gaatgtattg caatcacctt cacgtccagg tctggggctg tttttactgc
     8701 tgcttggccc gctaatgtgg tttggcattg tctatttcgg ctcgctgctg acactgttat
     8761 ggcagggatt ttatactttt gacgatttca ccatgtcggt aacgccggaa ctgacgctgg
     8821 cgaatatccg tgcgctgttt aatccggcga attacgacat cattctccgc acgctgacta
     8881 tggctgtggc agtcactatc gccagcgcca ttctggcttt tccaatggcg tggtatatgg
     8941 cgcgctatac cagcgggaaa atgaaagcgt ttttatattg cggtaatgtt gccgatgtgg
     9001 gcgagctaca ttgttaaagc ctatgcctgg acgttattgc tggcaaaaga tggcgtggct
     9061 cagtggtttt tacaacatct tgggctggaa ccactgctga ctgcgttcct tacattacct
     9121 gcggtgggcg gaaatacgct gtcaacttcc gggctggggc gctttctggt gtttctctat
     9181 atctggttgc cgttcatgat cctgcccgtt caggcggcgc ttgagcgttt gccgccgtca
     9241 ttgttgcagg cgtcggctga tctcggcgca cgtccacgac aaacctttcg ctatgtggtg
     9301 ctgccgctgg caatcccggg tattgccgct ggctctatct ttaccttctc actcacactg
     9361 ggcgatttta tcgtcccgca gctggttggt cctccaggat attttatcgg caatatggtt
     9421 tattcccagc agggggcgat tggcaatatg ccgatggcgg cggcattcac cctggtgccg
     9481 attattctca tcgcactgta cctggcgttc gtgaaacgtc tgggagcgtt cgatgcactc
     9541 tgaacgcgca ccgtttttcc tcaaactggc ggcctggggc ggcgttgttt tcctacattt
     9601 tcccatcctg ataatcgccg cctatgcgtt taacactgaa gatgcggcgt ttagttttcc
     9661 accgcagggc ctgacgctgc gctggtttag cgtggcagca cagcgtagtg atattcttga
     9721 tgccgtgaca ctgtcactta aagtggcggc gctggcgaca ttaattgcgc tggtgttagg
     9781 gacgctggca gctgccgcgc tgtggcgacg agactttttc ggcaaaaacg ccatttcgct
     9841 gttactgctg ctgcccattg cgctgccggg cagcgtcact ggtctggcgt tattaaccgc
     9901 ctttaaaacc atcaatctgg agccgggatt tttcaccatc gtggtcggtc atgcgacttt
     9961 ttgtgtagtt gtggtgttta acaatgtcat cgcccgtttt cgccgcacct cctggagtct
    10021 ggttgaggcg tcaatggatc ttggggccaa tggctggcaa accttccgct acgtagtgtt
    10081 gccgaatctc agttcggcgt tactggcagg aggaatgctg gcgtttgcct tgtcgttcga
    10141 tgaaatcatc gttacgacct ttacggcagg tcatgaacga acgttactgt tgtggttgct
    10201 caatcagctt gggcgaccgc gtgatgtacc ggtaactaac gtggtggcac tgctggttat
    10261 gttggtaaca accttgccga tcctgggggc ctggtggcta acccgcgaag gcgacaatgg
    10321 tcaataacca ctgatacagg aatatgctat gcaacataag ttactgatta acggagaact
    10381 ggttagcggc gaaggggaaa aacagcctgt ctataatccg gcaacggggg acgttttact
    10441 ggaaattgcc gaggcatccg cagagcaggt cgatgctgct gtgcgcgcgg cagatgcagc
    10501 atttgccgaa tgggggcaaa ccacgccgaa agtgcgtgcg gaatgtctgc tgaaactggc
    10561 tgatgttatc gaagaaaatg gtcaggtttt tgccgaactg gagtcccgta attgtggcaa
    10621 accgctgcat agtgcgttca atgatgaaat cccggcgatt gtcgatgttt ttcgcttttt
    10681 cgcgggtgcg gcgcgctgtc tgaatggtct ggcggcaggt gaatatcttg aaggtcatac
    10741 ttcgatgatc cgtcgcgatc cgttgggggt cgtggcttct atcgcaccgt ggaattatcc
    10801 gctgatgatg gccgcgtgga aacttgctcc ggcgctggcg gcagggaact gcgtagtgct
    10861 taaaccatca gaaattaccc cgctgaccgc gttgaagttg gcagagctgg cgaaagatat
    10921 cttcccggca ggcgtgatta acatactgtt tggcagaggc aaaacggtgg gtgatccgct
    10981 gaccggtcat cccaaagtgc ggatggtgtc gctgacgggc tctatcgcca ccggcgagca
    11041 catcatcagc cataccgcgt cgtccattaa gcgtactcat atggaacttg gtggcaaagc
    11101 gccagtgatt gtttttgatg atgcggatat tgaagcagtg gtcgaaggtg tacgtacatt
    11161 tggctattac aatgctggac aggattgtac tgcggcttgt cggatctacg cgcaaaaagg
    11221 catttacgat acgctggtgg aaaaactggg tgctgcggtg gcaacgttaa aatctggtgc
    11281 gccggatgac gagtctacgg agcttggacc tttaagctcg ctggcgcatc tcgaacgcgt
    11341 cagcaaggca gtagaagagg cgaaagcgac agggcacatc aaagtgatca ctggcggtga
    11401 aaagcgcaag ggtaatggct attactatgc gccgacgctg ctggctggcg cattacagga
    11461 cgatgccatc gtgcaaaaag aggtatttgg tccagtagtg agtgttacgc ccttcgacaa
    11521 cgaagaacag gtggtgaact gggcgaatga cagccagtac ggacttgcat cttcggtatg
    11581 gacgaaagat gtgggcaggg cgcatcgcgt cagcgcacgg ctgcaatatg gttgtacctg
    11641 ggtcaatacc catttcatgc tggtaagtga aatgccgcac ggtgggcaga aactttctgg
    11701 ttacggcaag gatatgtcac tttatgggct ggaggattac accgtcgtcc gccacgtcat
    11761 ggttaaacat taagttagat gttagtggct gttataggac ggccactatc gttttaggtt
    11821 ctgagtttaa tggcggttaa gtattagaat gattacatta ataactagcg taaaaattag
    11881 gctaaatgtt tgatggcgat ttttcatcct gtcacagttg attagcataa ggctttagag
    11941 tatcgttatt aaactaataa tttataagta gaagtattaa caatgaaagt tcaattgtac
    12001 aaattcacgg aagataaaaa taaaaccctt accttcagat ggacgaaaaa acactttgag
    12061 ttttgtatgg ataataaaat
//
LOCUS       DAZPLI010000122        11925 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZPLI010000122 DAZPLI010000000
VERSION     DAZPLI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11925
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.123"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            154..720
                     /locus_tag="ACHUU3_003159"
     CDS             154..720
                     /locus_tag="ACHUU3_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2083160.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            876..2908
                     /locus_tag="ACHUU3_003160"
                     /pseudo
     CDS             876..2908
                     /locus_tag="ACHUU3_003160"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            2885..3229
                     /locus_tag="ACHUU3_003161"
     CDS             2885..3229
                     /locus_tag="ACHUU3_003161"
                     /inference="COORDINATES: protein motif:HMM:NF025319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC C-terminal domain-containing protein"
                     /protein_id="HFR2083161.1"
                     /translation="MNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGKDVNVIIRNA
                     SGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQHCSLHLPEHM
                     EDTANRLILPCH"
     gene            3244..3975
                     /locus_tag="ACHUU3_003162"
     CDS             3244..3975
                     /locus_tag="ACHUU3_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR2083162.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3972..5033
                     /locus_tag="ACHUU3_003163"
     CDS             3972..5033
                     /locus_tag="ACHUU3_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2083163.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVTPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5185..6231
                     /locus_tag="ACHUU3_003164"
     CDS             5185..6231
                     /locus_tag="ACHUU3_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR2083164.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6228..7019)
                     /gene="nei"
                     /locus_tag="ACHUU3_003165"
     CDS             complement(6228..7019)
                     /gene="nei"
                     /locus_tag="ACHUU3_003165"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR2083165.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7055..7789)
                     /gene="pxpA"
                     /locus_tag="ACHUU3_003166"
     CDS             complement(7055..7789)
                     /gene="pxpA"
                     /locus_tag="ACHUU3_003166"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR2083166.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7779..8711)
                     /gene="pxpC"
                     /locus_tag="ACHUU3_003167"
     CDS             complement(7779..8711)
                     /gene="pxpC"
                     /locus_tag="ACHUU3_003167"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFR2083167.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(8705..9361)
                     /gene="pxpB"
                     /locus_tag="ACHUU3_003168"
     CDS             complement(8705..9361)
                     /gene="pxpB"
                     /locus_tag="ACHUU3_003168"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR2083168.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9384..10127)
                     /gene="ybgI"
                     /locus_tag="ACHUU3_003169"
     CDS             complement(9384..10127)
                     /gene="ybgI"
                     /locus_tag="ACHUU3_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR2083169.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10398..11879
                     /gene="dtpD"
                     /locus_tag="ACHUU3_003170"
     CDS             10398..11879
                     /gene="dtpD"
                     /locus_tag="ACHUU3_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR2083170.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         2874 a         3175 c         2901 g         2975 t
ORIGIN      
        1 tattcatcaa acatttaatt cataccatca attaattact tttacattta ataaaattaa
       61 taatattttt aagaaaaact aaaaacattc gacaatcaaa taagatattc ttgcaccgat
      121 atactttttt cttttctgtt aaaggactca gttatgttta aaggacaaaa aacattggcc
      181 gcactggccg tatctctgct gttcacagca cctgtttatg cggctgatga aggatccggt
      241 gaaattcact ttaaaggtga agttattgaa gcaccgtgtg aaatacatca ggatgatatt
      301 gataaagagg ttgaactcgg tcaggtgacc accagccaca ttaatcagtc acatcacagc
      361 gatgccgttg ctgtcgacct gcgcttagtc aactgtgatc tggaaaactc cagcaacggt
      421 tccggtggca agatttctaa agttgcagtc acctttgata gctcagcgaa aaccaccggc
      481 gcagatccga ttctcaacaa caccagcacg ggtgaagcca ccggcgtcgg cgtacgttta
      541 atgaataaag atcaaagcaa tatcgtttta ggcaccgcta ctccagatat cgacctggct
      601 ccgacctcca gcgaacaaac gctgaatttc tttgcctgga tggaacaaat tgatcaggct
      661 acacctgtaa cgccaggcgc agttacggcg aatgcaacct acgtgctgga ttataaataa
      721 gtattacttt ggcggcgcta tcgccgcctt tcataaaata tttatcaggt atggacaccg
      781 tgaatattta tcgactctct tttgtatcct gcctggtcgt ggcgatgcct tgcgcattgg
      841 cggtcgaatt caaccttaat gttctcgata aatcgatgcg cgaccgcatt gatatttcat
      901 tattaaagga aaaaggagtc attgctcccg gtgagtattt tgttagcgtt gcggtaaata
      961 ataaccaaat cagtaacggg caaaagatta actggcacaa aaatgacgat aaaaccattc
     1021 cgtgcatcaa tgatttactg gtcgataaat ttggcttaaa acctgaagtc cgtcagtcgt
     1081 taccattgat aaatcagtgc gtcgatttta gctcccgacc tgaaatgctc ttcaatttcg
     1141 atcaagccaa tcagcaactg aatattagta ttccgcaagc ctggctggcg tggcactcag
     1201 aaaactggac cccaccctcc acatggaaag aaggtgtcgc cggtatcctg atggattaca
     1261 acttgtttgc cagcagctac cgcccacagg acggcagcag cagcactaac ctgaacgcct
     1321 acggtaccac cggaattaac gccggggcat ggcgcttacg tagtgattat cagttgaatc
     1381 agactgatag cgatgataac catgaacagt caggcggaat atcgcgcacc tatctttttc
     1441 gtccattacc gcaattaggc tctaaattaa ccctcggcga aacggatttt agttccaata
     1501 ttttcgacgg tttttcttat accggcgcgg cactggcaag tgacgagcga atgttgccat
     1561 gggaactacg cggctacgcc ccacaaatta gcggtattgc acagaccaat gccacggtga
     1621 cgatcagtca atcaggccgc gtcatttacc agaaaaaagt cccaccaggc ccatttatca
     1681 ttgacgacct taatcagtct gttcagggca cactggatgt caaagtgacg gaagaagatg
     1741 gtcgggtgaa caatttccag gtttcggcag catcgacgcc cttcctgact cgtcagggac
     1801 aggttcgcta taaactggcc gcgggtcagc cacggccctc catgtcacat caaactgaaa
     1861 atgaaacctt ttttagcaat gaagtttcct gggggatgct gtcaaacacc tcgctgtacg
     1921 gcggcctgct gctttctggt gatgactacc attctgccgc aatgggtatt gggcaaaata
     1981 tgctgtggct tggtgcgctg tcgtttgatg tcacgtgggc cagtagccat tttgatactc
     2041 agcaggacga gcggggctta agctaccgtt ttaattacag caaacaagtg gatgccacta
     2101 acagcacgat ttccctcgcc gcttatcgtt tctccgatcg tcattttcac agctacgcca
     2161 actatctgga tcacaaatac aacgacagcg atgcgcagga cgaaaaacag acgatcagct
     2221 tattgtgggc caaccgatta ccccactaaa cctcaatctt tacgccaacc tgctacatca
     2281 aacctggtgg aatgcagacg cctccacgac cgccaacatc acggcaggtt ttaatgttga
     2341 tattggtgac tggagagata tctctatttc gacgtcattc aatacaaccc attacgaaga
     2401 taaagatcac gacaaccaga tttacctgtc gatttcgctc cccttcggta acggtggtcg
     2461 ggttggctat gacatgcaaa acagtagcca cagcaccaca caccgcatgt cgtggaacga
     2521 tacgctggat gaacgtaata gctggggcat gtctgccgga ctgcaatccg accgtcctga
     2581 caatggagcc caggtgagcg gtaactatca gcacctgagt tcagcgggtg agtgggatat
     2641 ttctggtacc tatgccgcca atgattacag ttccgtcagc agcagctgga gcggttcttt
     2701 caccgcaacc caatatggtg cagcgtttca tcgccgcagc tccaccaatg aaccacgcct
     2761 gatggtcagc accgatggcg tggcagatat tccggttcag ggcaatctcg actacaccaa
     2821 ccattttggc attgcggtgg tgccgttgat ttccagttac cagccttcca ccgtggcggt
     2881 gaacatgaat gacttacccg acggcgtaac agttgcagaa aacgttatca aagaaacgtg
     2941 gattgaaggc gcgataggtt acaaatcact ggcttcccgt tccggtaaag acgttaacgt
     3001 catcattcgc aacgccagcg gtcagtttcc tcccctcgga gcggatatcc gccaggatga
     3061 cagcggcatt agcgtgggga tggttggcga ggaaggacat gcctggttaa gcggagtcgc
     3121 tgaaaatcaa aagtttaccg tggtctgggg tgatagccag cattgctcgc tccatcttcc
     3181 tgaacatatg gaagacaccg caaatcgcct gattttacct tgtcattaat taaggaaatg
     3241 aaaatgacat ttatgaaagg actacccctt ttgttgttgg ttgccagtct gtgcagccac
     3301 gctgcactac aacccgatcg cactcgtatt gtgtttaacg ctaatgataa agctaccagt
     3361 ctgcgggtgg ataatcgtag cgataaactc ccctacctcg cttattcctg gctcgaaaat
     3421 gaaaaagggg agaaaagcga tgatcttctg gtcgccctgc cgccgataca acgtctggaa
     3481 ccgaaagcaa caacgcaggt acggatagta aagcaagcct cgaccacaaa attaccgggc
     3541 gatcgcgaaa cgctattttt ctacaatatg cgtgaaattc ctcccgcccc ggaaaaaaac
     3601 agcgaccatg ccgttctgca agtcgccatt cagagtcgta tcaaggtgtt ctggcgaccg
     3661 gctgcattac gcaagaaagc aggagaaaag gtcgaactgc agttacaggt cagccagcag
     3721 ggcaaccaac tgacactgaa aaatcctacc gcgtattatc tgaccattgc ctacctggga
     3781 cgaaatgaaa aaggcgttct ccccggcttc aaaaccgtga tggttgcgcc ttttagtacg
     3841 gtcaacacaa acaccggaaa ttatagcggc agccagtttt atctcggtta tatggatgat
     3901 tacggcgcgt tacgcatgac gacgctcaac tgcagcggac aatgccattt acaagccgtg
     3961 gaggcgaaga aatgagtgct ggcaagggag tgttgctcgt catctgtctg ttatttctgc
     4021 cgttgaagtc tgcgcttgcc ctgaactgct actttggttc atccggtggc tcggtagaaa
     4081 aatcagaagc gattcaacct tttgctgtac caggcaatgc caaactcggc gataagattt
     4141 gggaatctga cgatattaaa attcccgtct attgtgacaa caataccaac ggcaattttg
     4201 aaagtgagca tgtttatgcc tgggtaaccc cctacccggg ggtgcaggat cgctattatc
     4261 aactcggcgt aacttacaat ggcgttgatt atgacgccaa ccagggaaaa agccgcatcg
     4321 acactaacca gtgtattgac agtaagaaca ttgacattta tacccctgag cagattatcg
     4381 cgatgggatg gcagaacaaa atttgctcgg gtgatcccgc taatattcac atgtcgcgta
     4441 cctttctcgc tcgcatgcgg ttatacgtca aaatacgaga aatgccgccg catgattatc
     4501 aaagtacgct tagcgactat atcgtcgtgc aatttgacgg tgccggtagc gttaacgaag
     4561 accctactgc ccaaaacctg aaatatcata ttactggtct ggaaaacatt cgcgtgctgg
     4621 attgcagcgt caatttttcc atttcaccgg aaacacaggt gattgatttt ggtaaattta
     4681 atttgctgga tattcgtcgc cacacaatgt cgaaaacatt cagtattaag acgacaaaat
     4741 cacaaaatga ccaatgcacc gacggattta aagtcagttc ctccttttat accgaagaaa
     4801 cgttggttga agaagataaa gcattgttga ttggtaatgg tctgaagcta cgtttgctgg
     4861 atgaaaacgc ctcgccttat accttcaata aatatgctga atacgccgat ttcaccagcg
     4921 acatgttggt ctacgaaaaa acctatacag ctgaactttc gtccattgca ggtaccccca
     4981 tcgaggctgg gcccttcgat acagtggtgc tttttaaaat taactataac tgatttgcca
     5041 tgatgccctt tgcagccgga aagggcatct ctttcagaaa attgctgatt ttcccctttt
     5101 ccttgattgc tgttattttt tcctggcccg gctttcttta tgctgtatcg atacaccaaa
     5161 gagaaaataa tgagggagcg aaggatgcca gttttgcagt ggggaatgtt atgtgtgcta
     5221 tcacttctcc tttctattgg tttcctcgcg gtacatctcc cggcggcgct attacttggg
     5281 ccgatgatcg ccgggatcat cttcagtatg cgcggaatta ctctgcaact tccccgctcc
     5341 gcttttctcg ccgcgcaggc cattcttggc tgcatgattg cgcaaaacct cactggttcg
     5401 attctcacca cgctggccgc caactggccg atcgtgcttg ctattttact ggtgacgctg
     5461 ctttccagcg ccatcgtggg ttggttattg gtgcgctata gctcactgcc tggaaatacc
     5521 ggtgcctggg gttcttctcc aggcggcgca gcggcaatgg tcgctatggc gcaagattac
     5581 ggcgcagata ttcgcctggt tgcatttatg caatatctgc gagtgctgtt cgtcgcgggg
     5641 gccgctgttc tggtcacccg catgatgctg ggtgataacg ctgaagcggt taaccagcaa
     5701 atcgtctggt tcccgccagt gagcattaat ctcctgctta cgattttgct cgcagtcgtt
     5761 gccggtacgg cgggatgcat gctgcgtctt ccttccggca cgatgctcat cccgatgctg
     5821 gcgggcgcag tgctccagtc tggtcagctc atcaccatcg aactaccgga atggctgctg
     5881 gcgatggcgt atatggcaat tggctggcgg attggtcttg gtttcgataa acaaatctta
     5941 ctgcgggcac tacgcccgct accgcaaatc ctgctgtcga tttttgctct gctggctatt
     6001 tgtgcgggta tggcgtgggg gctgacccgg tttatgcaca ttgattttat gactgcctac
     6061 ctcgccacca gtcccggcgg gcttgatacc gtggcggtca tcgccgcagg gagcaatgcc
     6121 gatatggcgc ttattatggc gatgcaaacc ctgcgcctgt tcagtatttt gctgacgggg
     6181 ccagccattg cgcggtttat ttcaacctat gcgccgaagc ggtcggccta gtgctggcag
     6241 ccagggcacc agtaaaacgg gcgagatgac agcgtggttt tctcaatgat gctgccacag
     6301 cgttcgcacg gttcgccatc acgatgaaaa accttaaagc gaaacagcgc cccatgatgc
     6361 ttattctcat ccacctgccc cctcgtagcg taggaaaatc gaggaatatc cagtaacgcg
     6421 tgtgcgagtg catccagttg cgccgcattg agatctttcg ctttatgatt tccagtcaac
     6481 ccaacctgcc agaggatctc cacccgcaaa taattgccaa gcccagccag aaacgcctga
     6541 tcgagcagta atcctgcaaa ctgacggtta cgaaagcgcg gtgacaataa ccgttccttt
     6601 accacctccg gcgtcagatt cggatccagc acatcgggac caacgcgttg taaaaacgga
     6661 tgcgtggtca gttgttccgg cctcaacatc tcaatatccg aggcgctata aagcagaatg
     6721 gttttgtcag ccgtttgcag ttttacccgc aatactcgcg tggtctgcgg ctcttcgccg
     6781 gtatcaacca cgcgccagac gccgtaaagc tgattatggc tgtagagcgt taagtcgttg
     6841 gaaaaatgag ttaacaacgc cttaccacgc gtttccacat gggtaacgtg ttgaccgata
     6901 agttgtgatt gataagtttt taactgcggg aaggcaaacc agacatcagt tagtggtttg
     6961 cctttgatcg ccgcctccag gttatccgct gcacggcgga tctccgggcc ttcaggcatg
     7021 gtgatatcct tttttcattc tttattaatc ggggttatgc tgcgacaaca atctcctttt
     7081 cggcaaatgt agagcgtagt cggcgggcga acgccagtgc gtgctcgccg tcgccatgca
     7141 ggcagactgt ttgcgccgtg accgttgccc attcgccggt gatacttttg actctgccgt
     7201 gttgcaccat ctctagcgtt tgcgccaacg cctgttcttc gttttcaatc aacgcgcctc
     7261 gctgacttcg cggcaccagc gagccgtcag cctgataacc gcgatcggca aacacttcct
     7321 cgcgcgttgt caggccatat tgcttgcctg cacgaatcag ctcgcttcct gccagcccaa
     7381 cgagaatcaa tgccggatcg caagcgtata ccgctctggc gatggcgtct gccagttgtg
     7441 cttctttcgc cgcctggttg tacaacatgc cgtgcggttt gacatgacgc attacgccgc
     7501 cttgcgcacg ggcaatcgtt gccagcgcgc caatttgata cagcgtctgg gcgtaaacgg
     7561 tttctggcgg cagctgcatg gcgctgcgac caaaattttc cctgtcggga aaactcgggt
     7621 gagcgccaat cgcgacacca ttttttattg cttcacgcac gcaagcctgc atgatttgcg
     7681 catcgcctgc atgaaatcca caggcaatat tggcagagga aaccagcgtt aatagctctg
     7741 cgtcgctggc gcagccttcg cccagatcgg cgttcaggtc aattttcatt gtgcagccgc
     7801 cacactaatt gttcgaaata acgttgctga tcttgccgcg ctttcagtgc ctcttccagt
     7861 gaacactgga caaaatgaat cggctgaccg agcggaattt gcgccagatg gtacatatca
     7921 gcctcaatga tacaggcaat acgcgggtaa ccatcggtgg tctgtgcgtc gttcatcaac
     7981 acaatcggct gcccgttatg tggcacctgc accacgcccg gtaacaaacc gtgagataac
     8041 agttcgcgat cggtggtgcg ttttaaaatt tgcccctgta agcgatagcc catgcggtta
     8101 ctttgcgagc taagctgcca gggcgaacgc cagaatgcat cctgcgaggc gcgatcgaac
     8161 tcatgatatt ccggccccgg caaggcgcga atgcggttgc cccacagcag ctgtttaacg
     8221 ccctgcgctt ccataaaatc acgcttcgat ttgccaatcg ggagtcggtc accatccttc
     8281 agtaaacggc cttccagccc gccaatcccc actttgagat cggtgctgca tgaccccatt
     8341 accggcggaa catcaatacc acccgcgacc gccagataac tgcgcatccc gtgctgcggg
     8401 cgtttaagcg ttaaacgctg gcctgctttc atcggcaatc gccagccggt ccagacggca
     8461 ttatcatcca gccgcgcttc gcaaccggca cccgtcagag caaaccaccc atcagtttcg
     8521 aactcaaccg ttaactgacc gagcgtgatc tccagcgcgg gggcattggc gtcattaccc
     8581 accagcaggt tagcaatgcg taacgcaggc atatccagtg cgccgcagtg gctgattccc
     8641 gactggcgaa aaccgtgacg accgccatcc tgcacagtgg tatacatgcc cgcacgaata
     8701 atcttcagca tactccctcc ttctgcggta caaagcgcac gctgtctccc ggacgtaata
     8761 agatgggttc gtcacgcgcc ggatcaaaca ggctgagtga ggtatgacca atcaactgcc
     8821 agccacccgg cgttgccagc ggataaacac cagtctgcgg cccgccgatc ccgacagaac
     8881 ctgccggaac gagcaagcgc ggttcagcgc gccgtggcgt gtgtagttgt tccggcaaac
     8941 tcccgagata cgggaagccc ggttgaaaac ctaaaaacca gaccacgtat tccacggagg
     9001 agtgcaattc aacaacctgt ttttcgctaa acccgcaatg cgccgcgacc accgccaaat
     9061 ccggtccgcc tgcaccaccg taaaccaccg gaatttcaat aaagcgagac tcaggttcca
     9121 gggcctcgct ctcctcccac cagcgttgca aacgctctat ggcatccagc gccagcgact
     9181 caggattacg caaaatcacc gtgatattgt tcatgccggg aatggcttca accacattcg
     9241 gcatatccac cagacgctgc gccagccgcc agatccgttt ctggctagcc agcgtcaccg
     9301 gcggttccag ttccagcact accgccgttt cacctatcag ataacaacgc gctcgttgca
     9361 ctttcgatcc ctctgattat tcgttatgca ggattaggaa tatcaataaa ggtcacatca
     9421 agatcggtat tttcattcag ccactcgctc aatgcgcgaa taccaccacg ttcagtggca
     9481 tggtgacctg cagcataaaa atgcaacccc tgctcgcggg ctgaatgaat ggtctgttca
     9541 gaaacttcgc cagtaataaa agcatccacg ccaaaacgcg cggcgctatc gataaaactt
     9601 tgcccgccgc ccgtgcacca ggcgacgcgc tggaccacct caggtccggt atcgccacac
     9661 cataacggct tacgtcccag acgcgcttca atccaggagg ctagttccag tcccggcaca
     9721 ggcatggtca gctctcccca cggcaccaac ggctcaattt cgcccatgac cgtgatcccc
     9781 agtaacgccg ccagctgtgc gttattgccc agctcaggat gtacgtcaag cggcaaatgc
     9841 cagccataca ggttgatatc attcgccagc aacgttttta aacggttacg cttcattccg
     9901 cgaatgaccg gagactctcc tttccagaag tagccatgat gcacaatgac cgcatccgcg
     9961 cccagacgca ctgcctcatc gagcaaagcc tggctggcgg tgacaccggt aacaattttt
    10021 tgcaccgtct ctttgccttc tacctgcaaa ccattcggcg cgtaatcact aatcgccgcg
    10081 ctgttcagtt tttcgttaat cagttgttcc agttcggtgt ttttcatctt cgcgccttcc
    10141 gtattctgat agctacaccg ttaacatagc gttacctcgc ctgttcgtcg atagccaatc
    10201 ttttgacata ttaagaaaca aacccgacgc caaaagacgc aaattgttta tatcatcacg
    10261 cttcagctaa tgtatattta tgcaatcaga cagcatattg cataaataat cagatctgta
    10321 tcaaagtttt aactttaagg aaacacaagc acgccgcgca actattacgt gacgttgcag
    10381 tcaattgtgg aattattatg aataaacacg catcacaacc gcgcactatt tactatgtcg
    10441 ttgcgctgca aatctgggaa tattttagct tttacggcat gcgtgccctg ctgattctct
    10501 atctcaccaa tcaactaaaa tacaacgata ctcacgccta cgagttattt agcgcctact
    10561 gttcgctggt gtatgtcacg ccaatcctcg gtggcttttt ggcggataaa gttctcggca
    10621 atcgcatggc ggtgatgctg ggggcgttgt tgatggcgat cggtcatgtg gtgctgggtg
    10681 ccagtgagat ccatccgtca ttcctctatc tgtccctggc gattatcgtt tgtggctatg
    10741 gtctgtttaa atccaatgtc agttgcctgc tcggcgagct gtatgagcca accgatccgc
    10801 gtcgtgatgg cggtttctcg ctgatgtatg cagcgggtaa cgtggggtct attatcgcac
    10861 ctatcgcctg tggttacgcc caggaagagt acagctgggc gatgggcttt ggcctggcgg
    10921 cggtgggtat gatcgcgggt ctggtcattt tcttatgtgg caatcgtcat ttcacacata
    10981 cccgcggcgt taataaaaaa gtactgcgtg caacaaactt tctcctgcct aactggggat
    11041 ggctgctggt tctgctggtg gcaacgcctg cgctgattac cgtactgttc tggaaagagt
    11101 ggtcggtata cgccttaatt gtcgcgacta ttattggcct gggtgtactg gcaaaaattt
    11161 atcgcaaagc agaaaaccag aaacagcgta aggagctggg gctgattgtg acgctcacct
    11221 tcttcagtat gttgttctgg gccttcgcac aacagggcgg cagctcaatt agcctttata
    11281 tcgaccgctt cgttaaccgc gatatgtttg gttataccgt tccgactgct atgttccagt
    11341 cgattaatgc cttcgcagtt atgctatgcg gtgtgttcct ggcgtgggtg gttaaagaaa
    11401 gcgtcgcagg taatcgtacc gtgcgcatct ggggaaaatt tgctcttggc cttggcctga
    11461 tgagcgccgg attctgcatt ctgaccttaa gcgcccgctg gtccgcaatg tatggtcact
    11521 cttctctgcc actgatggtg ttaggcctcg cggtgatggg ctttgcggaa ctgtttatcg
    11581 acccggttgc catgtcgcaa attacgcgca ttgaaattcc gggcgtgacc ggcgtattaa
    11641 ccggcatcta catgctgctt tctggcgcga tcgcgaacta tctggcgggc gtgattgccg
    11701 atcagacatc gcaggcttcg tttgatgctt ccggggcgat caactactcc atcaatgcat
    11761 atattgaagt atttgatcaa attacctggg gcgcactggc gtgtgtagga ctggtactga
    11821 tgatttggct gtatcaggcg ctgaaattca gaaaccgcgc gctggcgctg gagtcataat
    11881 aggccagcac cgatgcctga tgcgacgctt gacgcgtctt atcag
//
LOCUS       DAZPLI010000123        11779 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZPLI010000123 DAZPLI010000000
VERSION     DAZPLI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.124"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..611)
                     /locus_tag="ACHUU3_003171"
     CDS             complement(<1..611)
                     /locus_tag="ACHUU3_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR2083171.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
     gene            complement(734..2380)
                     /gene="fumB"
                     /locus_tag="ACHUU3_003172"
     CDS             complement(734..2380)
                     /gene="fumB"
                     /locus_tag="ACHUU3_003172"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR2083172.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="ACHUU3_003173"
     CDS             complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="ACHUU3_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR2083173.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="ACHUU3_003174"
     CDS             complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="ACHUU3_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR2083174.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5085..6716)
                     /locus_tag="ACHUU3_003175"
     CDS             complement(5085..6716)
                     /locus_tag="ACHUU3_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR2083175.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6897..7127
                     /gene="yjdI"
                     /locus_tag="ACHUU3_003176"
     CDS             6897..7127
                     /gene="yjdI"
                     /locus_tag="ACHUU3_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR2083176.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7139..7411
                     /locus_tag="ACHUU3_003177"
     CDS             7139..7411
                     /locus_tag="ACHUU3_003177"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR2083177.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7638..7934
                     /gene="ghoS"
                     /locus_tag="ACHUU3_003178"
     CDS             7638..7934
                     /gene="ghoS"
                     /locus_tag="ACHUU3_003178"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR2083178.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7962..8135
                     /gene="ghoT"
                     /locus_tag="ACHUU3_003179"
     CDS             7962..8135
                     /gene="ghoT"
                     /locus_tag="ACHUU3_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFR2083179.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="ACHUU3_003180"
     CDS             complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="ACHUU3_003180"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR2083180.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="ACHUU3_003181"
     CDS             complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="ACHUU3_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFR2083181.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11524..>11779)
                     /locus_tag="ACHUU3_003182"
     CDS             complement(11524..>11779)
                     /locus_tag="ACHUU3_003182"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR2083182.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3043 a         2858 c         2894 g         2984 t
ORIGIN      
        1 aagcgccgta gctggcggca atgaaagcga gcgaggaata tcaccgtcgt taatgcgcca
       61 agcagcgcgc agaacatatc ggattgcgtg tcccactggt cgccctgggt gccgagaaaa
      121 tcatccgctc cctggcccat tgccagcgcc gcccaccact cgattaattc atacatcgcg
      181 cttatcgcca gcgccacgca gcagactagg aacgccacca ttttacgtcc gcgcacgtac
      241 atcccgcgca cgagaatttc tcgtgccacc agtgcaggca ccagcccctg gaaaaaatgc
      301 cccagcttgt catacggatt acggctcaac cccagccatt cctgcacctc aaaaccgatg
      361 gggactttcg cgtaggtgta ttgcccgccg accatcagga tgatggcgtg aaagaaaatg
      421 agcgtataga gcagcggcgt taacggataa cgtttagcgg tggcaagcag tagcggcacg
      481 acaataatca ccggtgtcac ttccatcagc caggtgagtt tgtcatgggc cgaaatgccg
      541 gtataaatca ggattaacgt tagcgccagt gcgccggtgt taagaattaa cggtttgagt
      601 gtgcgggtca tggtgagttc gcaagtcagg gaaaatactg actattcacc agcaagcatg
      661 aaattacaat cgcaaaagag gtggcccagg gggatcacct ggcagcatgc tgccaggcgc
      721 tgggccgaag aggttactta gtgcagttcg cgcactgttt gttgacgatt tgctggaaga
      781 agtcgttacc tttgtcatcg accaggataa acgccgggaa atcttctact tcgattttcc
      841 agatagcttc catacccagc tccggataag cgacgcattc cagatgcttg atgctctgct
      901 gcgccagtac cgccgccgga ccgccgatgc taccgaggta gaagccgccg tgtttatgac
      961 acgcgtcggt aacctgctgg ctgcggttgc ctttcgccag catgatcatg ctgccgccgt
     1021 gggattgcag cagatccaca taggagtcca tacgacctgc ggtggttggg ccaagtgaac
     1081 ctgatggata accggcaggg gttttcgccg gacccgcgta gtagatcggg tgatctttga
     1141 tgtactgcgg aagttcttta ccggcgtcaa tcagctcttt cagcttggcg tgtgcaatat
     1201 ctcgacccac aataatggtg ccagtgagcg acaaacgagt ggaaaccgga tattgcgaaa
     1261 gctgggcgag gatctctttc atcgggcggt taaggtcgac cttcaccgct tcgccttcac
     1321 cggcctgacg cagttcttgt ggaatgtact gacctggatt gtgttccagt ttttcgatcc
     1381 agataccttc gcggttgatt ttcgctttaa tgttacggtc agcggagcag gagacgccca
     1441 tgcctaccgg gcaggatgcg ccgtgacgtg gcagacggat aacgcgaatg tcgtgcgcga
     1501 agtatttacc gccaaactgc gcgccaagac cgagtttctg ggcctcttcc agcagttcct
     1561 gttccagctg gacatcgcgg aacgcctgac catgttcgtt cccttccgtc ggcagttcat
     1621 cgtaatagtg agcgcttgct aatttgacgg ttttcaggtt ggtttccgca gacgtaccgc
     1681 caatcacaaa cgcgatatgg tacggcgggc aggctgcagt accgagggta cgcattttct
     1741 cgacgaggaa gtttttcagt ttgccgggag tcagcagggc tttggtttcc tggtagagat
     1801 acgttttgtt ggcagagccg ccgcctttcg caacgcaaag gaatttgtac tcatcgccat
     1861 ctaccgcgta caggtcgatt tgcgcaggca ggttagtgcc ggtgttgacc tctttgtaca
     1921 tgtccagcgc cgcgttttgc gagtagcgca ggttatcttc gatataggtg ttatagacgc
     1981 ctttcgacag cgcttcttca tcaccgccac cggtccacac gcgctggcct tttttaccga
     2041 cgatgatcgc ggtgccggta tcctggcagg tcggcagcac gcctttggcg gcgatttcgg
     2101 agtttcttaa gaattgcagt gccacgtact tgtcgttttc gctggcttct ggatcgtgaa
     2161 gaatagccgc aacctgtttc tggtgtgccg gacggagcat gaaagaagcg tcgtgaaagg
     2221 cttgctgcgc cagcagggtc agggcttctg gttccacttt caggatggtt tcgccgtcga
     2281 agtcggcaac gctaacgtaa tcggaagtga gtagatagta ttcggtattg tctttcccca
     2341 tcgggaaagg tgcctggtag ataaagggtt tgtttgacat agcttccagc ctgtaactct
     2401 gtatttgtta ttcgaaaatg gcgtgccgcg tagtcacggg cggcacgcaa agtgcattta
     2461 taagaacccg tacatcgcgg cgaagatcca gccgaagacg cacgatacgc tcacaccaat
     2521 caaccccggc agaataaagc tgtggttgat gacgaagcga ccgatgtggg tggtgccgga
     2581 acggtcaaac tgaatcgctg ccagatcgct cggataagtc ggcaggatgt aataaccgta
     2641 gcaagccggt gctgaagcca cgatgtatgc cggatcaacg ccgatcgcca gcgcgaccgg
     2701 aacaatcgcc gccagcgccg cagcctgaga gtttacaaac ttggaaacca gcagcagaac
     2761 aatggcatag gcccacggat actctttcac catttcaccc agtacgccct gaatttcaga
     2821 catatgcgca ccgaacatgg tttctgccat ccatgcgata ccgtacaccg ccacgatggc
     2881 gatcataccg gaacggaaga cttcgttttt tgagatagac gcgggattgg ttttggtcag
     2941 gataataatc agcgccccgg tcagcagcat aaacatctga ataaccagta ccatcgacag
     3001 cggtttgccg ccgaaggatg gacgcaggtc cgaatcagca ccaagaaggg cgactacagc
     3061 gattgccccg aggaaaatcc acattgccag ccagttgctt ttcggcagtt ttttgtccag
     3121 cagcgtcgcg gtatcaccgt aaacatactc acggttttcc ggtacggaga tgaatttctg
     3181 gaactcttcg tctttatcca gatctttacc acggaaccag ctgaagatac cgatcgccag
     3241 gataccgatt aacgtcgatg gaatggtgat tgccagcagg tcgaggaact caagatggcg
     3301 accatcaaag gtgacattac ccagcattgc aaccagagac acgaccgcaa ccgacaccgg
     3361 actggcgatg atccccatct gtgcaccgat agaacttgcc gccatcggac gttccggacg
     3421 gatgttgttc ttaatggcga cgtcgtagat gatcggcaga atggtgtaaa ccacatgacc
     3481 cgtaccgcaa agaatggtca gcgtacaggt cacaaacggc gcgacaattg agacatattt
     3541 cgggttgcgg cgcagcagct tctcggcaat ttgcagcatg acatcaagac cgcccgaagc
     3601 ttgcaaggtc gccgatgccg ccaccaccgc aatgataacc agcatgacat caaccggtgg
     3661 tttacctggc tgaaggtgga agacgaagac cagaatgacc agaccgatac cgcctaataa
     3721 acccagcgcg ataccaccct ttctggcacc ataaaacaga catatcagta ttatgataag
     3781 ttggatagta aataacatgt gtgaaccctc gcgataatcc tatttaaatt tttgctgaat
     3841 agatcacagt cacgttctgt tttgtatgaa ctgttttcag agcctgacta aatatccgtt
     3901 tggtcgttac cggctttagc aaatacctca cagtgaatat tggctgatta aagcagcggc
     3961 aaatttatgc actgtgataa gcggcttttt cagagagaaa gactctctgg cgatggggtt
     4021 atagtttgta ctgcgcagat gttaatgttt tgtttgctgt tatcttattg atatttatcg
     4081 gtctattttt tgttctcagg gcagatgtca ttaggtttat agattaatct gatctaccca
     4141 tttgtgggta aaaatacaca taatgcgggt gacataatag ttaattaact tttgttagcg
     4201 ttttgaaatt aaaaacaccg ttcacctgaa gagatattaa tttttagcga tgatgcaggg
     4261 ataattatat ttgatctggc acaagtttta ctgatgaagg atgtaacttg tgccaggggt
     4321 attttgcatt acggtaataa ttattactta cagataacca gcgatccgtt attggcaata
     4381 ttgtttcagt agtgagtagt gttctgcctg aatacggtaa cggtaaactg gacgccccgt
     4441 gacgccataa tggatactgg tgaacaagat gtggcagttg accagccaga tgaggtattt
     4501 acggcaggaa acacgcgaaa tgttaacctc gttggctagc tcgtcggttg aaaattcata
     4561 gtcctgatgc gcgtcaatcc actggcacag tgtgcgtaac gtctgcggcg ttaagccttt
     4621 tggcaagcga cgaggatcct gttcgttgga gctgctgccg tggattagct gatcaagctc
     4681 ggcctggtca taatactgat gtttttccag cgccattttc ttttgccgcc agtcggtgag
     4741 cgcctcttca aagcgggaag cctggaaggg tttgatcagg taatccacga caccgtaatg
     4801 cagcgaatct ttaatggttg ccgcatcggc tgcggaggag atgacaatca catcactttt
     4861 gcaacgcgcg ttatgcagga caggcagtaa atcgagcccg ttctcttttt gcatatagat
     4921 atcgagcaat atcaggtcga taggcgtatc gctattgaag ataatctctt tggctttctc
     4981 cagcgtcgag gctgttccac agcattgaaa gcctgggatt tgtgctacgt atcggcgatt
     5041 cagctccgcg accattgcgt cgtcatcgat aattaataca ttgatcatct gttcgacctc
     5101 tccccgtccc agggtatctg gacaaaaaat tgtgtgaaaa tcccgggttc cgattccacg
     5161 gcgatgctgc cgccgagatt ttctacctgt tgtttgacaa gtgctaaacc gacgcctcgc
     5221 tcgcttcctt ttgtcgagac acctttgtca aaaatgtgat cgattttgtc gggtgcgatc
     5281 cccggtccat catcgttaac ttcgcagtgc agccagccgt gacggtagtg caatgttacg
     5341 ctaatttcgc caccgggttc cggccctaat gcttccagcg cgttttctat cagatttccc
     5401 aatgtggtaa tcagcgtcgc gacctggtcc tcactgccgc tgtctggcag ctggctttca
     5461 ctgtttaaaa tcagcgtatg gcctaaatcg gtcgcgcgat taatcttgct gattaaaaaa
     5521 ccagcgataa ccggagattt gatcttaccc agcagagagc caatctcttc ctgatagtta
     5581 ttggctgttt ttagaatgta atcttccaac tgcttataac tcttcagatg caataatccg
     5641 agaatcacgt gcaatttatt cataaattcg tgggatcgtt cacgaagtgc gtcggcatag
     5701 ttgaccagac cgtcgagtcg ctgcatcagt ttacgtactt cggttttgtc cctgaaggtt
     5761 gaaatggcac cgatgataac gccattactg cgcaccggaa cggtgttgat cagtaatagc
     5821 cggtctttaa tcgtaatctc ttcgtcgcgg cgcggcgtac cgtcgcgtaa cacttccgag
     5881 acatctacca cctgtgacca tgagtggctg agcgtcgaca gtttctcatc gtcctgcgac
     5941 ttacggtaat tcagcaattc ttgtgcggca tcgttgatca gcgtgacctc gccgcgatcg
     6001 tccacggcaa cgacgccttc tttgatagac tgcaacatgg cctggcgttg ctcaaacagc
     6061 gtggagattt cgtagggttc caggccgaaa aggatttttt tcagtacctt aaccagaatg
     6121 caggtgccaa tcagtccgac cagcatgcca aataataccg accagataat gctccagcga
     6181 ctgtcattga tttgttgggt cacgcggctt aactcaaggc cgatcgccac cacgccaatt
     6241 tgtttatgat tttcatcgta gatgggggta aatacgcgta aagcctgcgc cagaaaaccg
     6301 cgattgatag cgacattttc tttgccattc agcgctttaa ggatgtcatc acctttaaat
     6361 ggctgaccaa tacgctgggc ttcaggatgc gagtagcgaa gactttgcat atcggtaacg
     6421 acaataaaca gcagatcgtt gcgtttgcgc acggcttccg cgatggcctg gatgccgctc
     6481 tcctgcggtt ttttctgcaa gccctgacgg atttccggcg agttggcgag ggtacgcgcc
     6541 actgccagtg ccttgttggc tagcccgtct cgcgtcatat cactgatttg cgagaagtaa
     6601 atcagatgca ccaccaatag caccgagaac agtaccgcac tgaccattaa gatcactgtg
     6661 gtactcaatt tcatcggacg tttgcgtaac atgcggtagg gcaatgaatg tctcatcagc
     6721 ttccttgtgt gacaaatttc ttaaacatta tctctgatga ggcgggtaat tcaaagggag
     6781 taagaatgat tggctatata ggggaagaga ttctggcaac ggaaactgcc agtgctgtat
     6841 gaagattcca gggctatgct tatagcgata atcatactga tgagagaggg aaagtcatgg
     6901 atcaggcgct actggacgag ggttatcgct gttataccgg cgaaaagatc gatgtctatt
     6961 tcaacactgc gatatgtcag cattctggca attgcgtacg tggcaacggc aagttattta
     7021 atctcaaacg aaagccgtgg atcatgccgg atgaagtcga cgtcgccact gtggttaaag
     7081 tgattgatac gtgcccgagc ggcgcgctga aataccgtca taaataagcg agggtaaaat
     7141 ggaaatacgc gaaggccaca ataaatttta cattaatgac gaacaaggca agcaaatcgc
     7201 tgaaattgtc tttgtgccga ctggagagaa tttagcgatt atcgaacata ccgatgtcga
     7261 tgaaagcctg aaagggcagg ggattggtaa acagctggtt gcaaaagtcg tggaaaaaat
     7321 gcgtcgggaa aaacgaaaaa ttatcccact atgcccattt gcgaaacacg aatttgataa
     7381 aacgcgggag tatgatgata ttcgcagttg atgggagagt acagagtcac gatatttttc
     7441 atgctctccg cggagtgatg caggagagca tctgaagggt agggggatgc acaaagaatg
     7501 ggcagagagc gcgttttttt gtcccaagtc atccccttta ctgagcaaaa aaaagaatat
     7561 ctcctatatg agaatcatca atcggggtta tcaagttttg cgtccccaga gcattcaata
     7621 ttgataggag tcatattatg gaaggtaaaa acaagttcaa tacttatgtt gtttcttttg
     7681 attatccatc atcttattcc tcagtgttct taaggttaag atcattgatg tatgatatga
     7741 atttctcttc tatcgtggct gatgaatatg ggataccacg acaattgaat gaaaattcct
     7801 tcgcaataac gacatcgtta gccgcaagtg aaatcgaaga tttaatcagg cttaaatgct
     7861 tagacttacc ggatattgat tttgacctca acattatgac agttgatgac tatttccgtc
     7921 agttttacaa gtagccagga aggataagaa gaacataaaa aatggcacta ttctctaaaa
     7981 tattaatttt ttatgtgatt ggtgtgaaca tatcctttgt cattatctgg tttatctcac
     8041 atgagaaaac acatattcgt ttacttagtg cattcctggt cggaataacc tggccaatga
     8101 gtctgcctgt ggcattactt ttttctctct tttaggagct gtagttgagt ggacccgctg
     8161 gttatcgtga aacgaagcgt ctctcctcaa aactggattt gcggaacgac gaacgaagca
     8221 gtcgcctgac tgcttcgttc attaaagtga aatttatttc tgtgggcgca tcgccgggaa
     8281 gagaataacg tcgcgaatag tatgactgtt tgtaaacagc ataatcattc ggtcgatacc
     8341 aatacccaga ccagcggttg gcggcagacc atattccagc gcagttacgt aatcttcgtc
     8401 atagaacatg gcttcgtcgt cacctgcagc tttagcgtta acctgttcct ggaaacgttc
     8461 agcctgatct tctgcgtcgt ttaattcgct aaaaccatta ccgatttcac gaccaccgat
     8521 gaagaattca aaacggtcgg tgatttccgg gttaacatca ttacggcgcg ccagcggtga
     8581 cacttctgcc ggatattccg taataaaggt tggctgaatc agatgtgctt ctgccacttc
     8641 atcaaagatc tccgtgataa tacgtcccag cccccagctt ttctctaccg taattccgat
     8701 agattcagct aatgctttag cagcatcaaa attatccagg tcggccatat cggtttctgg
     8761 acgatatttt ttgattgctt cgcgcatggt gagtttttca aacggtttgc cgaaatcaaa
     8821 cacatgctcg ccataagtga ctttagtggt acccagaacc tcttgtgcca gggtgcggaa
     8881 cagtgactct gtcagttcaa tcaaatcgtg gtaatccgca tacgccatgt agagttccat
     8941 cattgtgaac tcaggattat ggcgaacaga aataccttca ttacggaagt tacggttgat
     9001 ttcgaatacc cgttcaaaac cgcctacaac cagacgtttc agatacagct ccggcgcgat
     9061 acgcagatac atatctaaat ccagagcatt atgatgggta ataaacgggc gagcagatgc
     9121 cccacctgga attacctgca tcatcggggt ttctacttcc ataaagccgc gcgcgaccat
     9181 gaactgacgg atagcggcca gaatttttga acggacaaca aacgtttgac gggatttatc
     9241 gttagcgatg aggtccagat aacgttgacg ataacggact tcctgatcct gcagaccatg
     9301 gaatttatct ggtaaaggac gtagtgcttt agtcagcagg cgcaactcag tacagtgaat
     9361 ggaaagctca cccgtttgcg tcttaaacag cgtaccgcgg gcaccgataa tgtcacccag
     9421 atcccatttt ttaaactgat cgttataaac accttctggc aggctatctc ttgcaacgta
     9481 cagttgaata cggccaccga catcctgcaa cgttacaaag gaggctttcc ccatgatacg
     9541 acgggtcatc attcggccag caaccgagac ttcaatgttt aaggattcca gttcctggtt
     9601 attcttcgca tcaaactctt cgtgcaactg gtcagaggta tggtcgcggc gaaaatcatt
     9661 gggaaacgcc acaccttgct gacgtagtgc cgccagtttt tcgcggcgat ttctcagttc
     9721 atcgttaaaa tcaatagcct cattggctcc ccgtgtttct tgttcagaca ttttggttcc
     9781 tctaaatcca gctttcaatt tagcgtagat aaagagacag atcggtacac tgtattatct
     9841 gcctcgacta ttaataactc agtttatctt atcgaaacta acgcttacct caagttgata
     9901 tcactaataa ataaccatca aaatggttta aatgacttat taatctcgat ttactagtgt
     9961 gattaactga aagttaattt attcataacc aattgttttt acgacagtta atcgttgctc
    10021 tcctgtatca tattcgtcgg cgtgctaaac agaaaacggg tagcaaaggc caatacgacg
    10081 atgatcgcga cacaagccaa cgtccactct cccatctgag aaaagaatcg ctggtaagct
    10141 gcaattacca taccgctaat ttgcgactct gtcgtctgct gtgccacgac gcctgccagc
    10201 cagttggcga ccgcgcctgt tgccagcata taaataccgg ttaatacgcc agacattttc
    10261 agacgcgtga tttgcgcaat cgccaccggg tcaataaaga gttctgcaaa gcccattagc
    10321 gccaacccgg atatcatcac gcccattgac gcttgaccgt cagccgctgc atgtcgggca
    10381 tcaaatgcca acaacataaa gccacaagcc atcagcagta agccaaaggc aaacttcagc
    10441 cagacgcgca atgttgagtt gccgcggctt tctggcgacg ccagccaggc cagtacaacc
    10501 ccagcgagca tcactgcaat tgcattcacc gactggaata gtgctgtagg tacttcaata
    10561 ttgaatgcct gacgattcac aaagcgatcg ataaacaagc tgatggtact gccgccctgt
    10621 tgtgccagta cccagaacaa cgtcccgaca aacatcaaca atacaatttg ccaaagagca
    10681 cggcggtgtt cggggaattt gatcatcatg cgggcaatga tttgtgcggc aatgaggcaa
    10741 acgatcgcca gcaaatatcc ggaccagtcg ttctccagca gcagggtaaa aaatactggg
    10801 gctaaacaga gcatcaccac taaccagctc catactggta aggcaaattt gacgctagtg
    10861 agcgcttttt tatccatact acgcgtggac tggaaatgac gatgaccgct taagaaaatc
    10921 aacaaaccga taaacatgcc gccacccgca agggcaaagc caacatgcca tccataccac
    10981 tgagcagcca ggccgcaggc gattggggcg gcgatagaac cgatattgcc cgcagcatac
    11041 agcagcgaaa aaccgccatc acgtcgatga tcgttctcgt cgtagagctc gccaagcaaa
    11101 cagctgatgt ttgatttgaa taaaccgtag ccacaaataa tgattgccag cgccagatac
    11161 aggctaaagg ttgaatttgt atcaatgccc agcaccacat ggccaagggt cattaacagc
    11221 gcgccggcaa tcactgcagt gcggttgccg agcaggcggt cggcaagcca gccgccgaga
    11281 ataggggtaa cgtaaaccag agaagcatat gcgctgaaca ggctgatggc atggttatca
    11341 tcaaaaccaa gctgatgggt gagatagaga atgagtaagg cacgcatgcc gtaaaaactg
    11401 aagtactccc agatttggat cgccacgata tagtatatcg cgcgcggctg tgagggtgtt
    11461 ttcatgtgtt ctccttatga gcaaaaaagg gaagtggcaa gccacttccc ttgtacgagc
    11521 taattatttt ttgctttctt ctttcaatac cttaacggta tagcggccat cagcctgacg
    11581 gtatgcaccg tgaatatcgg tttcaaatcc cggatagtga gcgccgattt cacacagcat
    11641 ctgcaggaac tccagaaccg gacggctttc ttcggtgatc atttcacccg gcattaccag
    11701 aggaactccc ggcggatacg gaaggatcat attggcgtta atacgaccta ccatttcgtc
    11761 gaggtaaact tcttcggtc
//
LOCUS       DAZPLI010000124        11542 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZPLI010000124 DAZPLI010000000
VERSION     DAZPLI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.125"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            460..1386
                     /locus_tag="ACHUU3_003183"
     CDS             460..1386
                     /locus_tag="ACHUU3_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFR2083183.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFERAEQTPGIKADWLV
                     RMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQARTLPDMLSSDAG
                     PLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(1336..2529)
                     /locus_tag="ACHUU3_003184"
     CDS             complement(1336..2529)
                     /locus_tag="ACHUU3_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2083184.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2665..4389
                     /gene="iucA"
                     /locus_tag="ACHUU3_003185"
     CDS             2665..4389
                     /gene="iucA"
                     /locus_tag="ACHUU3_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR2083185.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4390..5337
                     /gene="iucB"
                     /locus_tag="ACHUU3_003186"
     CDS             4390..5337
                     /gene="iucB"
                     /locus_tag="ACHUU3_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR2083186.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5337..7079
                     /gene="iucC"
                     /locus_tag="ACHUU3_003187"
     CDS             5337..7079
                     /gene="iucC"
                     /locus_tag="ACHUU3_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR2083187.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7076..8413
                     /gene="iucD"
                     /locus_tag="ACHUU3_003188"
     CDS             7076..8413
                     /gene="iucD"
                     /locus_tag="ACHUU3_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR2083188.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8419..10614
                     /gene="iutA"
                     /locus_tag="ACHUU3_003189"
     CDS             8419..10614
                     /gene="iutA"
                     /locus_tag="ACHUU3_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR2083189.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11165..11308)
                     /locus_tag="ACHUU3_003190"
     CDS             complement(11165..11308)
                     /locus_tag="ACHUU3_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083190.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2926 a         2896 c         2950 g         2770 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcataccctt acccacaagt tgaaataata
      421 gtattatatt gaaaagacag agattatttc agaggtgtta tggaaaactg ggttattcag
      481 gagctaaaat cactggatat aggggactca agacttgata aaagagtaaa acatatcctc
      541 aatagcctca gtcgttcacc agaagaaagt attcctgttt cctgccgtac ctggagtgaa
      601 acaaaggcag cttatcgttg tttttcaaac gataaagtct ctgctaataa aattatggct
      661 cctcacaaag aaaatatcac tgaaagagct cagcagttta aaagagtttt agttttacag
      721 gatacaacag aacttaatta ttctggtcag aaagaaaaac aaggagttgg tcacaaaaaa
      781 cataaagatg aaagaaactt atttcttcat cctcaactcg tcatcagtga gtcaggtgtt
      841 tgcctcggtg tttatgatga ctatcagtgg ttcagggatg aattaaaaac cagtaaaaat
      901 acccgacaag aaatctgcaa tgatttactt cataaaaaac atgtcagtga aaaagaaacc
      961 tggcgttggg ttgaaggata taataaagca acagaactgg ctcgccaatg ccctgatacc
     1021 catgtattgt caatttctga tcgtgaaggc gatttttatg acctgtttga acgcgcagag
     1081 cagactcccg gaataaaagc tgactggctt gtcagaatga aatttaataa cagagcaacg
     1141 ctgaatatta atggtaaacg ggatcatagg ttgttgcacg aaagaatcat ggagattaca
     1201 ccgcagcaac tggttgaatt cactatacct gatgggcggg ggcagcaagc cagaacactg
     1261 cctgacatgc tttcatcaga cgcaggtcct ctctcatatc ctctgattta ctccagtaag
     1321 gtgatgatca tatctttatt tgatagtacg gtaatgccag ataaatacag acacaatggt
     1381 aaataatata gttgcaaata atgctgccat aatcagcgcg attgcgacac ctgttttcga
     1441 taccagcggc accagcaaag cgcacagtcc atagccaagt gtgtgactgg tagcgagcca
     1501 gcccgcgcct gcgccatcag ccagcttatc attcagtaga agttgataag cgggtgttgc
     1561 cagagctgct ccaaatgaca gcactgcaca gcccgggtaa aacaaccata gctgttcgga
     1621 gagcatgata gccagaccac cactcatcaa cactccggca ctcaggagca gggccaccgg
     1681 agtcaggcgc tgcggacgga gtaccccgaa ctgcgcgata agcgcagcta ctgcggacag
     1741 acccaacagc caggccacct gttggctaat ggcggtggta tccgtgacga actggcgagt
     1801 aagggcaggc gaaagtccaa gctgcatcat gctcaccgcc gcagccagta atattgcgca
     1861 aagcagataa ggcagacaat cccgcttcag actgacgctc ttgcactccg gtgtgggctg
     1921 tggtggtgtg ccgggcaacc gcagcagcat cagcagcgcc agcactggtg ctgccatcag
     1981 tagccccagt ggcgccagag gatgaatggc cagcattgct gccgcgcaca gcggcccgaa
     2041 tagcctgccg caactcaggc cggagctgat ggttgccaga gcggccatgc gattcccttc
     2101 tccagctctc tgcaatgccc agacctgaca ggctggcacc attgccgaca cggtcagccc
     2161 gtaggcgatc cgggcgatga ttaatattcc cagtcccacc gctgttgtta tggcgctggt
     2221 agccatcagc acgcttccca gccctagcag ggtaaagctt atgccatatc ctcccagagc
     2281 ccacaacacc acaggtttac tgccagtacg ggaaatttgc tttccccacc acggggacga
     2341 tggcagaaac agcattgagc caagcatcag cagggcagcc catacagaca gactcagatt
     2401 tgtctgtatg accagaacag ggagcacaac cagcagaccg ttctgtccga taccgagaag
     2461 cccggcactg aacgcaagtg gccagcagga cagtgttttt tggggcgtat cttcgatccc
     2521 tggtgacatt ataatgtttc aactccatgt attaattgtg tttatttgta aaattaattt
     2581 atctgacaat aacatttatt gttgataatg agaatcatta tcgacataat tgttattatt
     2641 ttactgtgtg ggagctgttt gactatgacc ctgccctctg aaaaaccagc cacagatgtg
     2701 gctgcgcagt gcttcctgaa tgcactgatt cgtgaaacca cagactggaa actgacagaa
     2761 tacccgccag acgaattgct catcccgctg gatgagcaga aatcgctcca tttcagagtg
     2821 gcttatttct ccccaaccca gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca
     2881 tcaggcagtt atcctgtcga ctttaccact ctctcccggc ttattattga taagctacgc
     2941 catcaactgt ttctgcccgt tcccctctgc gaaactttcc accagcgcgt gctggaaagc
     3001 catgtccata cgcaacaggc aattgatgcc cgtcatgact gggccgctct gcgtgaaaaa
     3061 gcgttgaatt ttggcgaggc tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg
     3121 cctaagtctc atgaaccgtt taaccgacgg gaggctgaac gctacctgcc tgatatggca
     3181 ccccacttcc cactgcggtg gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg
     3241 catctcaacc ttcaacagcg gctgacgcga tttgccgcag agaatgcgcc tcagttactc
     3301 aacgaattaa gtgacaacca atggctgttc ccgttgcacc cgtggcaggg ggaatatctt
     3361 ttgcagcagg ggtggtgcca ggcacttgtt gctaaagggc tgattaaaga cttaggtgag
     3421 gccggcacgt cgtggctgcc gaccacctct tcccgttccc tctactgtgc caccagccgc
     3481 gatatgatca agttctccct gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg
     3541 aaagaagtga agcgtgggat gcgcctggca cgcctggctc aaaccgacgg ctggcagatg
     3601 ctacaggtcc gcttcccgac tttccgggta atgcaggagg atggctgggc cgggctgctc
     3661 gatcttaacg gcaacatcat gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg
     3721 gatcagccga aaagccagac taacgtactg gtctccctga ctcaggccgc acctgatggc
     3781 ggtgattcgc tgctggtttc ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg
     3841 caacaggccg cccatgcatg ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt
     3901 acggctgaag cggattacgg cctggtgctg ctggcgcatc agcaaaatat tcttgtccag
     3961 atgcttgggg atctgccggt cggatttatt taccgtgact gtcagggcag cgcttttatg
     4021 cctcacgcga cagactggct cgattctatt ggcgaggcgc aggcggaaaa tattttcacc
     4081 catgagcagt tgctgcgcta tttcccttat tacctgctgg ttaactccac ttttgctgtg
     4141 accgctgcgc tgggggctgc cgggctggac agcgaatcga atctgatggc tcgtgtacga
     4201 gcatcgctgg ctgaagtgcg tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg
     4261 gaaagtccgt actggaacgt aaaaggtaac tttttctgtt atctgaacga tcataacgag
     4321 aacaccattg ttgacccttc ggtgatctat ttcgatttcg ctaacccgct gcaggctcag
     4381 gaggtctgaa tgtctgaggc aaacattatt cacagcagat atggactgcg ctgtgaaaaa
     4441 ctcgacaagc ccctgaatct tggctgggga ctggacaata gcgcggtgtt gcactgtccc
     4501 ggggagctgc cgacagggtg gctgtgcgat gcgctggatc agatatttat cgccgcacca
     4561 caactgtcag cagtggctct cccctgggcc gaatggcgtg aggagccaca ggcgctgacg
     4621 ctttttgggc aggtaaaaag cgacattatc catcgtactg ccttctggca gttaccgtta
     4681 tggttgagtt caccggcaaa ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag
     4741 atttatttcc cgcagcgccc accccgtcca cagggtgagg tttaccgccg ttacgatcca
     4801 cgtattcgca ggatgctgag tttccgcatt gccgatcccg tttctgatgc agaacgtttc
     4861 acccgctgga tgaatgatcc gcgcgttgag tatttctggg agcaaagtgg ctcactggag
     4921 gtacagaccg cctatctgga gcgccagctc accggtaaac atgcattccc gctgattggg
     4981 tgcttcgacg atcggccatt cagctatttc gaaatctact gggcggcgga agaccgcatt
     5041 ggtcgtcact attcgtggca gccctttgac cgtggcctgc atctgctggt tggtgaacag
     5101 caatggcgcg gggcccacta tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg
     5161 ctggatgagc cccgtacgca gcgcacagta ctggagccac gcaccgataa ccagcgcctg
     5221 ttccgccatc ttgagcctgc gggataccgg acaattaaag agttcgactt cccacataag
     5281 cgctcgcgca tggtgatggc ggatcgtcat cacttcttca cggaggtcgg tctgtaatga
     5341 atcacaagga ctgggatttt gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg
     5401 agtatgagca ggttttccac gcggaatctc agggcgatga ccactactgc attaacctgc
     5461 cgggagcaca atggcgcttc atcgctgaac gtggtatctg gggctggctg tggattgatg
     5521 cccaaacgtt gcgctgcacg gacgaaccag tactggcgca gacgctgctg atgcagctaa
     5581 agccggtgct gtcaatgagt gatgccactg ttgcagagca tatgcaggat ttgtatgcca
     5641 cgctactggg cgacctgcaa ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga
     5701 ttaatcttga tgccgatcgt ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta
     5761 ataaaggtcg ccgtggctgg ggcaaagagg cgctggaacg atatgcgcca gagtatacca
     5821 acaccttcag actgcactgg ctggcggtaa aacgtgaaca tatgatctgg cgctgcgata
     5881 acgatctgga tattcagcag ttgttgactg ccgcaatgga tccgcaggag tttacacgct
     5941 tcagtcaggt ctggcaggaa aacgggctgg atcacaactg gctaccgctg ccggtacatc
     6001 cgtggcagtg gcagcaaaaa atcgccacag acttcatcgc tgattttgtc gaaggcagga
     6061 tggtgtctct cggcgagttt ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga
     6121 ccaacgccag ccgccgggga gggctggata tcaagctgcc gctgaccatc tacaacacct
     6181 catgctaccg gggaatccct ggcagatata tcgctgccgg accactggct tcacgctggc
     6241 tgcaacaggt ttttgcgacc gacgccaccc tggtgcaaag cggcgcagtg atccttggtg
     6301 aaccggctgc aggctatgtg tcccatgaag gctatgccgc gcttgcccgg gctccctatc
     6361 gctaccagga aatgcttggt gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg
     6421 atgaaagtcc tgttctgatg gcaacactga tggagtgcga cgaaaacaat cagccgctgg
     6481 caggcgcata tatcgaccgc tccggactgg acgctgaaac ctggcttacg caattgttcc
     6541 gggtggttgt ggttccgctg tatcacctgc tttgccgcta cggtgtcgcg cttattgcac
     6601 acggacaaaa tataacgctc gccatgaaag agggggtacc acagcgtgtt ctgctgaaag
     6661 acttccaggg cgatatgcgg ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc
     6721 aggaggtgcg tgatgccaca tcccgtctga gtgcggacta cttaatccat gatttgcaga
     6781 cgggtcactt cgtgacagta ctgcgtttta tttcgccact gatggttcgt cttggcgtac
     6841 ctgaaaggcg attttatcaa ctgctggcag cagtgttgag tgattacatg aacaaacatc
     6901 cacaaatggc agagcgtttt gcgcttttct cactcttcag gccacaaatc attcgcgtgg
     6961 tgctgaaccc ggtaaaactg acctggccgg acctggatgg cggcagtcgc atgctgccga
     7021 attaccttga gaatctgcaa aatccgctgt ggctggtaac tcaggaatat gaatcatgaa
     7081 aaaaagtgtc gattttattg gtgtcgggac agggccattt aatctcagca ttgctgcatt
     7141 gtcacatcag atcgaagaac tgaactgtct cttctttgac gaacatcctc atttttcctg
     7201 gcatccgggt atgctggtac cggattgtca tatgcagacc gtctttctga aagatctggt
     7261 cagtgcagtt gcacctacaa atccctacag ctttgttaac tatctggtga agcacaaaaa
     7321 gttctatcgc ttccttacaa gcaggctacg tacagtatcc cgtgaagagt tttctgacta
     7381 tctccgctgg gctgctgaag atatgaataa cctgtatttc agtcataccg ttgaaaatat
     7441 tgatttcgac aaaaaaagtc gattgtttct ggttcagacc agtcggggag aatattttgc
     7501 ccgcaatatc tgccttggta caggaaaaca accttattta ccaccctgtg tgaagcatgt
     7561 gacacaatcc tgtttccatg ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa
     7621 acggataacc gtggttggtg gaggacagag tggtgcagac ctgttcctta atgcattacg
     7681 cggggaatgg ggagaagcgg cggaaataaa ctgggtctca cggcgtaata attttaacgc
     7741 actggatgag gctgcttttg ctgatgagta ttttacacct gaatatattt caggcttctc
     7801 cggactgaag gaagatattc gccatcagtt actggatgag cagaaaatga catcggatgg
     7861 catcactgcc gattctttac tgaccattta tcgtgagttg taccaccgtt ttgaagttct
     7921 gagaaaacca agaaatatcc gtctgctacc cagtcgctcg gtaacaaccc tggaaagtag
     7981 tggtccgggc tggaggttat tgatggaaca tcatctggat cagggcagag agagcctgga
     8041 aagtgatgtg gtgattttcg ccacaggtta ccgttctgca ttgccacaaa tacttccctc
     8101 actgatgccc ttgatcacca tgcacgataa gaacaccttt aaagtgcgtg atgacttcac
     8161 tctggaatgg agtggcccga aagaaaataa catctttgcg gtcaacgcca gcatgcaaac
     8221 tcatggcatc gccgaacccc agctcagcct gatggcctgg cgatctgcac gtattcttaa
     8281 tcgcgtactg gggcgtgatt tattcgatct cagtatgccg cccgcactga ttcagtggcg
     8341 cagcggcagc cggaaaaaac cgcagccgga ggctgcttct ttaactcact atacagcaaa
     8401 tattcaggaa taacgatgat gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt
     8461 cttgtcgtgc tgtcaccagc tgtcgcccag caaaacgatg ataatgagat catagtgtct
     8521 gccagccgca gcaatcgaac tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat
     8581 gccgaactgg agcagcagat tcagggcggt aaagagctga aagacgcact ggctcagtta
     8641 atccccggcc ttgatgtcag cagccagagt cgaaccaact acggtatgaa catgcgtggc
     8701 cgcccgctgg ttgtcctgat tgacggtgtg cgcctcaact cttcacgttc cgacagccga
     8761 caactggact ctgtcgatcc ttttaatatc gaccatattg aagtgatctc cggcgcgacg
     8821 gccctgtacg gtggcgggag taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag
     8881 ccggaaacca tgatggagtt tgaggctggc acaaaaagtg gctttaacag cagtaaagat
     8941 cacgatgagc gcattgccgg tgctgtctcc ggcggaaatg accatatctc cggacgtctt
     9001 tccgtggcat atcagaaatt tggcggctgg tttgacggta acggcgatgc caccctgctt
     9061 gataacaccc agaccggcct gcagcactcc aatcggctgg acatcatggg aaccggtacg
     9121 ctgaacatcg atgaatcccg gcagcttcaa ctgataacgc agtactataa aagtcaggga
     9181 gacgacaatt acgggcttaa tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca
     9241 ccatacgtca gtaaggggct gaattctgac cgcattcccg gcactgagcg gcatttgatc
     9301 agcctgcagt actctgacag tgatttcctg ggacaggaac tggtcggtca ggtttactac
     9361 cgcgatgagt cgttgcggta ctacccgttc ccgacggtaa atgcgaataa acaggcgaca
     9421 gctttctcct cgtcacagca ggataccgac cagtacggca tgaaactgac tctgaacagc
     9481 caactgatgg acggctggca aatcacctgg gggctggatg ctgagcatga gcgctttacc
     9541 tccaaccaga tgttcttcga tctggctcag gcaagtgctt ccggagggct gaacaaccat
     9601 aagatttaca ccaccgggcg ctatccgtca tatgacatca ccaatctggc ggccttcctg
     9661 caatccagct atgacattaa tgatattttt accgttagcg gtggcgtacg ctatcagtat
     9721 actgagaaca gggtagatga tttcatcgac tacacgcagc aacagaagat tgctgccggg
     9781 aaggcgatat ctgccgacgc cattcctggt ggttcggtag attacgataa ctttctgttc
     9841 aatgctggtc tgctgatgca catcaccgaa cgtcagcagg catggttcaa tttttcccag
     9901 ggggtggcat tgccggatcc ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg
     9961 aacggccatc tttccctgac aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg
    10021 aaagtcgatt cttatgaact gggctggcgc tttaccggtg acaacctgcg gactcaaatc
    10081 gcggcatatt actcgctttc caataagagc gtggaaagga ataaagatct gaccatcagt
    10141 gtgaaggacg acaggcgccg tatttacggc gtggaaggtg cggtggacta cctgatcccg
    10201 gatactgact ggagtaccgg tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac
    10261 ggtcaatggc aaaaatatga cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt
    10321 aactgggcgc cggaaccgtg gagtctgcgt gtacagagca ccacttcttt cgacgtaagc
    10381 gatgcagagg gtaacgatat taatggttac actaccgtcg attttatcag tagttggcag
    10441 cttccggtgg gaacactcag cttcagcgtt gagaacctct tcgaccgtga ctataccact
    10501 gtctggggac agcgtgcacc tctgtactac tgcccgggtt acggccctgc ttcactgtac
    10561 gactacaaag gccggggccg aacctttggt ctgaactact cagtgctgtt ctgacgggga
    10621 tgcttttgca acaagggtat gctgtaaaca acatggtcct tggtggccat gttgtttaca
    10681 gccagtataa tatacagcct taacaagata ttccgtctcc ggaatggact gttcaccact
    10741 gaataacatt atttctgttt tatggatctt tgactgaaat ttatcgcata tcctcatttc
    10801 attgtccgtc tgaaaactaa ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc
    10861 ctgaaattcc tgagaggatg atctgcgccc aatatcctga gccggaactt tgctgcaatg
    10921 aaggccaacg aaaaatgggg gagtatgtta tagagtactt gctacagggt aaaaagaggt
    10981 acctgtcgct ggttctccgt tcagtttaac cgggagattt tctcctgcgc ctgtcataaa
    11041 gtccggtaat ggagattgtt aatacgatgg tgcacgatgc attgtcggtg cccggcccgg
    11101 aggatgcccc gtaactacat acggatcagg gttggcaata tcgaatggca ggctataggc
    11161 aaagttaaag ccccatggag tagcacaaaa tatgccacac aaaggaaacg gtctgaataa
    11221 cgcagtgatg gagaacttct tcggcacact gcaaaatcgc atcgtggtcg atcgctaatt
    11281 cgccgtgaat cactgaacga tgttccatgc gaacagatat tcgatcatat cgaaatatta
    11341 aacattaccc ctccccgcat tgggcaaaaa aaaaacaaaa aacgatcaat tagtattgat
    11401 tcacataatc tctcatcatt aatgagaaca ttgtcacata tttacaatat agaactgtta
    11461 cttttagtaa gctgttagta accaactgtt actaataatt ttaattctta atactttgtt
    11521 cattcaataa atgagttgag ag
//
LOCUS       DAZPLI010000125        11522 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZPLI010000125 DAZPLI010000000
VERSION     DAZPLI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11522
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.126"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..479)
                     /gene="sulA"
                     /locus_tag="ACHUU3_003191"
     CDS             complement(<1..479)
                     /gene="sulA"
                     /locus_tag="ACHUU3_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR2083191.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     "
     gene            698..1327
                     /gene="sxy"
                     /locus_tag="ACHUU3_003192"
     CDS             698..1327
                     /gene="sxy"
                     /locus_tag="ACHUU3_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFR2083192.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(1290..3452)
                     /gene="yccS"
                     /locus_tag="ACHUU3_003193"
     CDS             complement(1290..3452)
                     /gene="yccS"
                     /locus_tag="ACHUU3_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR2083193.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(3462..3908)
                     /locus_tag="ACHUU3_003194"
     CDS             complement(3462..3908)
                     /locus_tag="ACHUU3_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR2083194.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            4031..6085
                     /gene="helD"
                     /locus_tag="ACHUU3_003195"
     CDS             4031..6085
                     /gene="helD"
                     /locus_tag="ACHUU3_003195"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR2083195.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(6117..6575)
                     /gene="mgsA"
                     /locus_tag="ACHUU3_003196"
     CDS             complement(6117..6575)
                     /gene="mgsA"
                     /locus_tag="ACHUU3_003196"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR2083196.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(6671..7333)
                     /locus_tag="ACHUU3_003197"
     CDS             complement(6671..7333)
                     /locus_tag="ACHUU3_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR2083197.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            7506..7919
                     /locus_tag="ACHUU3_003198"
     CDS             7506..7919
                     /locus_tag="ACHUU3_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR2083198.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(7964..8281)
                     /gene="hspQ"
                     /locus_tag="ACHUU3_003199"
     CDS             complement(7964..8281)
                     /gene="hspQ"
                     /locus_tag="ACHUU3_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR2083199.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(8339..9529)
                     /gene="rlmI"
                     /locus_tag="ACHUU3_003200"
                     /pseudo
     CDS             complement(8339..9529)
                     /gene="rlmI"
                     /locus_tag="ACHUU3_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            9624..9902
                     /gene="yccX"
                     /locus_tag="ACHUU3_003201"
     CDS             9624..9902
                     /gene="yccX"
                     /locus_tag="ACHUU3_003201"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR2083200.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(9899..10228)
                     /gene="tusE"
                     /locus_tag="ACHUU3_003202"
     CDS             complement(9899..10228)
                     /gene="tusE"
                     /locus_tag="ACHUU3_003202"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR2083201.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(10319..10978)
                     /gene="yccA"
                     /locus_tag="ACHUU3_003203"
     CDS             complement(10319..10978)
                     /gene="yccA"
                     /locus_tag="ACHUU3_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR2083202.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(11185..11272)
                     /locus_tag="ACHUU3_003204"
     tRNA            complement(11185..11272)
                     /locus_tag="ACHUU3_003204"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11236..11238),aa:Ser,seq:tga)
BASE COUNT         3036 a         2835 c         2968 g         2683 t
ORIGIN      
        1 ccggaaagtt gtctcgtggc gtgagaggat gcgcttaccg gacgcataat aaaccccata
       61 gcgttacctt catttgccgc atcaacaagt tcagcatgct cttcttcagt caaatcatct
      121 gccaaccaac cgatcaccac actgtaattg cccgtgcgta aagcgcgaac cattgactcc
      181 acagtgtggc aaggggagag ctggctaatc tgcattactt tcgttaaggg tagcccagat
      241 gcctgaaccc attcccgact cagtttttgt tgcggtgtta accagagttg ccagcgcgat
      301 tgctgaccga gttgctgtaa caatggcaac agtagaagtt gcgtcatcat gggctgatct
      361 tcgcgataga caacttcact gataagcccg gctgtagtgt tttccgtaga gacacgcgca
      421 attttacttg ctgcggatga gaacgacgaa gaacgatgtg catagcctga agtgtacata
      481 atcaatccag cccctgtgag ttactgtatg gatgtacagt acatccagtg acaacaaaga
      541 tcaaccctat tttcggaaag agcctcgcaa attttgtcgt tggtgacggg aaaacataaa
      601 ttaatcttgc cccttaagaa taagttgcct attttcgtag ttaacggatc cgttaatgtg
      661 aatcattctt ttatgttatg attttaaaag gaattttatg aaaagcctct cctataagcg
      721 gatctataaa tcacaagaat acctggcaac gttgggcaca attgaatacc gatcattgtt
      781 tggcagttac agcctgaccg ttgacgacac ggtgtttgcg atggtttctg atggtgagtt
      841 gtatcttcgg gcttgtgagc aaagtgcaca gtactgtgta aaacatccgc ctgtctggct
      901 gacatataaa aagtgtggcc gatccgttac cctcaattac tatcgggttg atgaaagtct
      961 atggcgaaat caactgaagc tggtgcgtct gtcgaaatat tctctcgatg cagcgctgaa
     1021 agagaaaagc acgcgcaata cccgggaaag actgaaagat ttgcccaata tgtcttttca
     1081 tctggaagcg attctcgggg aggtggggat taaggatgta cgggcgttac gtatacttgg
     1141 ggcaaaaatg tgttggttgc gactgcggca gcaaaacagt ctggtgacag aaaagattct
     1201 gtttatgctt gaaggtgcca ttatcggcat tcatgaagct gcgctcccgg tggcacgccg
     1261 ccaggagctt gcagaatggg ctgactctct tacgccgaaa caggagtttc ctgcggaact
     1321 tgagtaatct ggcgttgcag acgaccaatc tcaggcagta atgcaatcaa taatccaact
     1381 tgttgtacga ccagaggttc tttgctgtct gcccgtggtt caagttgctg catccgctgt
     1441 ttcaggctag ctaatgcctc attgacgcgt tcttcatcag caggttgatg atgtaacgcg
     1501 tcatcaacat agcaaactgc gtcatcaaga aacgccagaa tttcaggatt agttaactgc
     1561 tcccggtgag caccgagggc tgagatatag ctggtaaacg tatggttaag gcacagcaac
     1621 cgaaacgcgg cttcgcgaat ttgcggggta acgttcggct cgctggacat atttgatacc
     1681 accgacgcca gctcagcatc acggttgtgt gcatcgcggc gggcaatacg atacgccaga
     1741 cggttatcac gcccctgatg gtattgctcc agtatggcat cgagataccg acagttggcc
     1801 tctgtggcgc gttcgagcat gcgcggcaga ttgcgaaact gccagtcagg ccagatgtag
     1861 ctcactgccg cccacgcaat ggcacaacca atcagcgtat cgattacgcg aggtaacgct
     1921 acttcaaaac cttcacccag taagttaaaa cacagtagca ccaaaagtgt gatgaacatc
     1981 gttgcatgag cgtattgcac gttacggaag gcaaaaaaga gcacgccggt aataaccagc
     2041 agcaccagct gcccttccag tgatggcaca aaccacagca caggaatgcc aatggcgata
     2101 cctaccagcg taccaataat ccttaacttc aggcggtggc gcgtggcgtt atagtttggc
     2161 tggcagacaa acaaacttgt cagcaagatc caatacccgt gatgcattcc ggttatctga
     2221 atgatggcgt agccgaagca caacaccagc gacattctta ccgcatgacg gaagagggcg
     2281 gattccggcg tgaagtgacg gctaagacgc agccagatat cactcaaccc gtgcgggctg
     2341 tcatcagcga gctcattttc gtcattatta tggggtagtg cctgggcctg ttctgattca
     2401 attgttgcca gttgggcatc aatggcgcgt aaattgttca gcaaaaatcc cagtgttttg
     2461 agtaaatcgg cgggtgcgcc gttatcgcgc atccgctcca gcgcagcatc aatatgcgta
     2521 aaagcgcgct caaaatgcgg atcatgttga taaggctgac gcaacaaaat acagcgtgac
     2581 agttgctggc acgcctggcc ctgcatcgac atcagccgct gaaaacggaa cagcacgtcg
     2641 ctgtggcgaa aatgttcacg caatgtttga tactgaatat gagaagagct ggcacgctcg
     2701 tgaatatcct gtgcgacaaa gtaataatgc agcgtgcgac gcgttccccg ttgaccacga
     2761 tcgccacgta agcgggtcag cagcgagagt ttcgtctgat tcaatgtcgc catcagcaga
     2821 ccgttggcga gagccaaatc gtacagcggt gcctggcttt catcttcaat atcaggatca
     2881 aacatgcgcg acttgagctc aagataacgc gccagttgtt catagcaacg cgccaggttg
     2941 tcctgcagcg ggcggaccgg gaacagcaga tgaccaataa gtgtcaggac gttgtaccag
     3001 acggcaccgg ccagcagata catcggctgc tgataccagt gctcatacag tgatgttccc
     3061 aacatagtgt aaatggcgat cagcaatgca ccgaaggcaa ttgttgcata gcgttgaccc
     3121 agaccgccga gcaaaatgaa gccgctggta gagagcgtta agccaatcgc aaatagccag
     3181 ggccagggaa acagcaattc tactgaggcc gaggcgataa aaaagcagaa cagcgtaatg
     3241 atgaggttac gcaaacgtcc cgccagtcgg tcatcgagat cggtcagcgc cgctgccacc
     3301 atccccagcg ttagcggaat cgtcagtttt acatcaccca gccaccacgg aaacgctgtg
     3361 gttccacaaa gcgcaataaa aatacgcgcg taatacagcc aggcgctgtt ccaggtatag
     3421 cgtttgagca aaggacttag cataaaggcc atattagtgg attattcaaa acgacgacgt
     3481 gcattggctt cacgcgcagc ttgcgctgtt tctaccgata ccacgcgacg accaaccggc
     3541 catagtgcaa tagcggcaat tttaaagttc gcaatgccga caggaatgcc aatgattgaa
     3601 atacattgtg cgatgcccgt tgcaatgtgc atcaggcata accaccagcc aaagaaaatc
     3661 agccagaaaa tattcaatac cgtaccgcca gtattcagca gcacattttt gccagccggg
     3721 ttcagttcat cgacatgaat agcttcattg ccataaggca ccagagatag tttagtgatc
     3781 tcccagcagg atcgtgtcag cggtaaggta aaaatcagca caatactgac cagagtcgcc
     3841 aacagccagc ccagagtggt ggcaaatccg ccaagcacaa agttcagaat gttcaaaacg
     3901 gtacgcataa aacctcgctt tactgtggtt ttcagtaatg ggcggcaatt gtaacgtttt
     3961 tttgggctgg agcacgtttt ctctgacggt tacactgata agaaataatt tcgtgtggat
     4021 ctacagagtc atggaactga aagcgacaac gcttggaaaa cgtctggcac agcaccctta
     4081 cgatcgggcg gtgatcctca atgccgggat taaagtctcc ggcgatcgcc acgaatacct
     4141 tattcctttc aatcaattac tggcgattca ctgtaagcgc ggtctggtat ggggcgagct
     4201 ggaatttgta ctgccggacg aaaaagtggt gcgtctgcac ggcaccgaat ggggcgagac
     4261 gcagcgtttt taccatcatc ttgatgctca ctggcggcgg tggagtggcg agatgagcga
     4321 aattgcgtct ggtgttttac gccagcaact ggatttgatt gccacgcgca ctggagaaaa
     4381 taaatggctg acgcgtgagc aaacctctgg cgttcagcaa caaatccgcc aggctttgtc
     4441 ggcgttgccg ttgccggtta accgactgga agaattcgat aactgccgtg aggcgtggcg
     4501 taaatgtcag gcctggttga aagatattga aagcgctcgg ttgcagcata accaggcgta
     4561 taccgaagcc atgcttaccg agtatgcgga ttttttccgc caggtcgagt cttcaccgct
     4621 gaatccggcg caggcccggg cagtcgttaa tggcgagcat tctctgttag tgctggcagg
     4681 tgcaggaagc ggaaaaacgt cggtgctggt ggcccgtgca ggctggttgc tggcgcgtgg
     4741 tgaagcgtcc cctgagcaaa ttttattgct ggcgtttggt cgcaaagccg ctgaagagat
     4801 ggacgagcgg attcgcgaac ggctacatac cgaagacatt accgcacgca cgtttcatgc
     4861 gctggcgctg catattattc agcagggcag caaaaaagtt ccgatagtca gcaaactgga
     4921 aaatgatacc gctgcccgtc atgaactctt tattgctgag tggcgcaagc aatgcagcga
     4981 aaagaaagcg caggcgaagg gctggcggca atggctgacg gaagaaatgc agtggtcagt
     5041 gccagaaggt aacttctggg atgatgaaaa attacagcgt cgccttgcct ctcgcctcga
     5101 tcgctgggta agtctgatgc ggatgcacgg tggtgcacag gcagaaatga ttgccagtgc
     5161 acccgaagag attcgcgatc tgttcagtaa acgtatcaag ttgatggccc cgttattaaa
     5221 agcctggaaa ggtgcgctga aggcagaaaa cgctgtcgat ttttcgggcc ttattcatca
     5281 ggcgattgtg attctggaga aaggtcgctt tatcagcccg tggaagcata ttctggttga
     5341 tgaatttcag gatatctcgc cgcagcgggc agcgttgtta gcggcattac gcaagcaaaa
     5401 cagtcagacg acgttgttcg ctgttggtga tgactggcag gcgatttacc gattcagcgg
     5461 tgcgcaaatg tcgctcacca ccgctttcca tgaaaacttt ggtgaaggcg aacgttgtga
     5521 tttagacacg acttaccgtt ttaacagtcg tatcggtaag gtggcaaacc ggtttattca
     5581 gcagaaccca ggccagctga aaaagccgct aaacagctta accaatggag acaaaaaagc
     5641 cgtcacgtta ttggatgaga gtcaacttga cgctttgctg gataagctct ctggttatgc
     5701 caaaccggaa gagcgcattc tgatcctggc gcgttaccat cacatgaggc ctgccagcct
     5761 ggaaaaagcg gcaacacgct ggccgaagtt gcaaatcgac tttatgacca ttcatgccag
     5821 caaagggcaa caggcggatt acgtcatcat cgttggcttg caggagggaa gtgatggttt
     5881 tccggctgcg gcgcgggagt cgattatgga agaggcgcta ctgccaccgg ttgaggattt
     5941 cccggacgct gaagaacggc ggttaatgta cgtggcgctg acccgggcac gccatcgggt
     6001 atgggcactg tttaacaaag agaatccctc tccctttgtg gaaatactga aaaatctgga
     6061 tgtgccggtg gcgagaaaac cgtaagaaac aggtggcgtt tgccacctgt gcaatattac
     6121 ttcagacggt ccgcgagata acgctgataa tcggggatta gaatatcgac cgcgtcgttg
     6181 aaatgcggcg actggattat gaagtctgcc gttgccacgt tggtggcgac cggaatgttc
     6241 cataccgtcg ccagacgcag caaggctttc acgtcaggat cgtgcggcac ggcatttagt
     6301 ggatcccaga agaaaatcaa tacatcaatt ttcccttctg agatcaatgc gccaacctgc
     6361 tggtcacccc ccattgggcc actcaacatc gcgttgacgt tcatgccggt cgcgcgggaa
     6421 attaagttac cggtagtgcc tgttgcatac agtacgtgtt gttccagtaa cggttgatgc
     6481 cgttccaccc agctcatcag catttgtttg cagtgatcgt gtgccaccag cgcaatatgt
     6541 ttccgcgcag gtaaagtgcg agtcgtcagt tccataatgt acatccgtag ttaactttcc
     6601 tacagattac tgtaagcact tatcgctgca agataaagac cgaaaaagcc tgcgcacagg
     6661 cacaaaaatc tcaggaagat ggttgttttt ccgcccactg caggaaagta tttcgcgttt
     6721 gtgggtcagc cagtttaaac caatacttca gccgttgttc tgtgagcacc tgagactgcg
     6781 gtggaatagc atccagctcg gaaacacccg aaagcagcgt actgtcatct gccatcatcg
     6841 tggcaaattg cggtagtgaa gcgcgtttcg cggatttgtt atagcgttca acctcgacct
     6901 cataatcaat cgtttttgcc gttgaggtaa tggcgagaat atccagcttt accggaatcg
     6961 gcgtcgcatc gccatccaac aattcaaggc gcggcgcggc atcaaaatgg ttagcttccc
     7021 gctcattctc caggcgaggc aggcgaaaat tgacctggtt gatgagctgg gtattaaaac
     7081 tgaccaccag cggtggggag atatacagcc gttcttcact attggaaaga tgaatcgtct
     7141 tctcaacgcg aaacactaac tgatgcggtc cattatccag ttcaatgctg tccgcaccgc
     7201 gcagcagaga gctggaaacc ttcttgccgt cgagtaccag aagatcgaca tcggttgaaa
     7261 gccgcagcgt ggtggcaaaa acggataccg gcaaacataa tgcaattaag gtggtcacga
     7321 tgccggtttt cataggtagc tcctgtcaaa agaccgcccg aataataatg tgtcaaaatt
     7381 tttctttaat catacttact aacatataga catatttccc gcttttgctc tcattcattc
     7441 gtattagctg catggttggc atgtcggctt tgtcgtacac tttgttaaac agtctggagg
     7501 aaataatgaa agaaaccgat attgcaggca ttttaacgtc cactcacacc attgcgctgg
     7561 tgggggcaag cgacaaaccc gatcgtccca gctatcgcgt gatgaaatat ctactcgacc
     7621 aggggtatca cgtcattccg gtttcgccaa aagttgccgg caaaacgctg ctgggccagc
     7681 aggggtatgg cacgctggca gacgttccgg aaaaggtcga tatggtcgat gttttccgta
     7741 attcagaagc ggcgtggggc gtggcacagg aagctattgc cattggcgcg aaaaccctct
     7801 ggatgcaact gggggtgatt aatgagcaag cggcagtact ggcacgggat gccgggttga
     7861 atgtggtgat ggatcgctgc ccggctattg agatccctcg cctgggcctg gccaaataaa
     7921 aaatccccgg aaggcaaaaa ccttccgggg attcgttcag ggatcagtta cgcagacgcg
     7981 gggcctggag ttgtttgcgg atggtctgcg ccagctcatc catggatggc tgttcaggat
     8041 gttcatcctg caattcgcta cttagctggg cttcagccag gtaggtatga accggtaggc
     8101 cgttatcgtc ctccatcact acgtgatacc acggagcggc gcgaagctcg tcatttaccg
     8161 ccagctcatc aggcgacggt tcagagagcg aataaaccgg gtcgatatcc acgaccactc
     8221 cgagataacc taacagggaa tggcggacct gctggccgat accgaatttg ctggcaatca
     8281 tagtcacctc ccgggaaatc tttacctttg atataagggt aaaattccac atttcaagtt
     8341 acatgacgcg acaggcaaac cctttcagat atagcccttc cggataggta gcgatcaccg
     8401 gatgatcggc tgcctgacgg aactgctcta taaattgtac atcacggccg gcatcaattg
     8461 cggcatccgc gatgattttc tgaaataaat cgctggtcat cagaccggaa caggagaaag
     8521 tcaggagaat accgccttca ttcagcaact gaatcgccag catgttgata tctttatagc
     8581 cacgacacgc gcccatcaac tggcttttat tctcaacaaa cttcggcggg tccatcacga
     8641 taacgtcaaa tttttcaccg cgatcgcgat aagtacgcag caatttaaag acatcatcac
     8701 ggacaaactc agccttgctc agatccagtt tgttcagctc aacgttctgc cgtgcaatat
     8761 ccagcgcttc ctaggaggta tcaacgctga caacctggct gcaaccgccc atcagtgccg
     8821 atacggcgaa accaccggta taggagaaac agttcagcac acgtttattt tcaacgtagc
     8881 ggcgggtagc caggcggctg tctcgctggt ccaggtagta gcccgttttg tgtccgtgct
     8941 gaatatccac cagcagcttc atgccgtgtt cttcaatcgg cagcagggca ggcggcaact
     9001 cgccggtgac gaggccctgg gtcagctcca tcccttcttt tttacgtacc gcaacatcgc
     9061 tgcgatcgta aatcgcacat tccgggtaca gcgtttgcag ggcactaatt aatgccgcgc
     9121 gctgatattc tgccccagca ctcagcagtt gcagcaccag aaaattaccg aaacgatcga
     9181 tagtaatacc cggcaggcca tcagattctc cggcgattaa acgatagctg tcgaggccat
     9241 ctttttgcgc cagtcagtca cgccattttt gtgcttgttg caaacggcgg gaaaaaaaag
     9301 caatgtcgat agactcagac gggtcaaacg tccagacgcg cgcccggatt tgcgaagctg
     9361 gcgaataagc gccgcgtgct aaccattttc cctgatgatc aacaatatcg atggtttcac
     9421 cgaggctggc tttaccttcc atgcgggcaa cggccccgga aaagacccac ggatggcgac
     9481 gaagtaatga tttttcgcgc cctttggcta acactaaacg tacactcata atttactttt
     9541 ctgtcgatgc aaagaaatgg gcgtcattgt ccggagtttt ccatggattt gcaacgcgtc
     9601 cattaaggat aaggagagca gcaatgtcga aagtctgcat aattgcctgg gtttacgggc
     9661 gagttcaggg cgtaggattt cgctacacca cacagtacga agcgaaaaga ctgggtttaa
     9721 ccgggtacgc caaaaatctt gacgatggca gcgtggaagt ggttgcctgc ggtgaagaag
     9781 ggcaggtgga aaaattaatg cagtggttaa agagtggcgg cccgcgttct gcgcgtgtag
     9841 aacgggtgct cagcgagccg catcatccct cgggggaatt aaccgatttt cgtatccgtt
     9901 aaatacattt taccggtttt ggcaggccag caattttggt ggcttgctta gccggacctt
     9961 tcggaaacag tcggtataaa tagcggctgt tacctttttc ttcgccaaat ttattcgcca
    10021 tcgcttttac cagcatacga atcgccggag aagtattgaa ttccagatag aaatcacgca
    10081 caaaacgcac cacttcccag tgttccggtg acagcgaaat cccttcgttc tctgcaatca
    10141 ccactgccag cggctcactc cactggctgc tttctttgag atagccttcg gtatccgttt
    10201 ctatctcttt accttcgaag atcagcataa ttattactac cttaatcaga ctggcggcag
    10261 tgtaacaaaa aacaaagccc cgcataaagc gaggctatga aagtgttagc gggtgagatt
    10321 aatcgcggct ggcgaagccc agaatgctca gcaggctgac gaagatgttg tacagcgaaa
    10381 catacaggct aaccgtggca cgaatatagt tcgtttcacc gccatgaacg atgttgctgg
    10441 tttcaaacaa aatagcgcca gaggagatca gaatgaagac cgcgctgatc gccagatgca
    10501 gagcaggcag ctgcaggaag atattagcaa ccataccaat cagcaccacc acaatacccg
    10561 ccatcagcat accgccgagg aacgacatat ctttgcgggt ggtcagcaca tatgcagagc
    10621 agcagaagaa cactaacgcc gttccgccca gtgccatagc gattacgtca cccattccgg
    10681 cagacagata ggtgttcaga ataggtccga ggatataacc cagaaaaccg gtaaaggcga
    10741 atgcggagat aatcccggtc ggcttattcg ccgttttata ggtcaggaac atcaaaccat
    10801 acatacccac cagcgtcaga atcagacccg gagatggcag catcagcacc gtactggcag
    10861 ttgcggtaat cgccgaaaag gccagcgtca ggctcagcag aaaataggta ttacgcagca
    10921 ccttatgggt gctaagcagt gatgtacggt catgtgaaga actaacaata cgatccattg
    10981 agtcactctc tatgacagat gtaattaatt aagcagcata atgataatgc gtaagggcac
    11041 ccagaagttt tacccatctt tacgcatttg atctggaaca ggtttaacag cggattatca
    11101 ggtcattaag caaatataac gccctgagaa tttcgacagg caaaagaaaa aggggttagc
    11161 atttagctaa ccccttatct tatttggcgg aagcgcagag attcgaactc tggaaccctt
    11221 tcgggtcgcc ggttttcaag accggtgcct tcaaccgctc ggccacactt ccggaatgac
    11281 gcgcactata aacatcccga tgcggcgtgt aaacccctaa tttgtttgtt tgcctgaaaa
    11341 acagccaaaa gtgcattgat agcgtgaaat aacagcagat tgatcatttc atcaccatga
    11401 attccttctc ttttactcgt ttagcaaccg gctaaacatc cccaccgccc ggcaaaaaga
    11461 aaaataggtc catttttatc gctaaaagat aaatccacac agtttgtatt gttttgtgca
    11521 aa
//
LOCUS       DAZPLI010000126        11428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZPLI010000126 DAZPLI010000000
VERSION     DAZPLI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.127"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHUU3_003205"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHUU3_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR2083203.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHUU3_003206"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHUU3_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR2083204.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHUU3_003207"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHUU3_003207"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR2083205.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="ACHUU3_003208"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="ACHUU3_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR2083206.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="ACHUU3_003209"
     CDS             complement(3928..5214)
                     /locus_tag="ACHUU3_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR2083207.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="ACHUU3_003210"
     CDS             complement(5235..6026)
                     /locus_tag="ACHUU3_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR2083208.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="ACHUU3_003211"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="ACHUU3_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR2083209.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHUU3_003212"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHUU3_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR2083210.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="ACHUU3_003213"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="ACHUU3_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR2083211.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="ACHUU3_003214"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="ACHUU3_003214"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR2083212.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="ACHUU3_003215"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="ACHUU3_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR2083213.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="ACHUU3_003216"
     CDS             complement(9882..10364)
                     /locus_tag="ACHUU3_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR2083214.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHUU3_003217"
     CDS             complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHUU3_003217"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFR2083215.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2805 a         2748 c         3059 g         2816 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgtt ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcactggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccaa ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat tatcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggcggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctacc aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtca
//
LOCUS       DAZPLI010000127        11293 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZPLI010000127 DAZPLI010000000
VERSION     DAZPLI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11293
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.128"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..741
                     /gene="hdhA"
                     /locus_tag="ACHUU3_003218"
     CDS             <1..741
                     /gene="hdhA"
                     /locus_tag="ACHUU3_003218"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR2083216.1"
                     /translation="DGKCAIITGAGAGIGKEIAITFATAGASVVVSDINADAANHVVD
                     EIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPKPFDMPMADF
                     RRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAASHL
                     VRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRLGQPQDIANA
                     ALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            962..1552
                     /gene="uidR"
                     /locus_tag="ACHUU3_003219"
     CDS             962..1552
                     /gene="uidR"
                     /locus_tag="ACHUU3_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR2083217.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1941..3752
                     /gene="uidA"
                     /locus_tag="ACHUU3_003220"
     CDS             1941..3752
                     /gene="uidA"
                     /locus_tag="ACHUU3_003220"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR2083218.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            3749..5122
                     /gene="uidB"
                     /locus_tag="ACHUU3_003221"
     CDS             3749..5122
                     /gene="uidB"
                     /locus_tag="ACHUU3_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFR2083219.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            5161..6471
                     /gene="uidC"
                     /locus_tag="ACHUU3_003222"
     CDS             5161..6471
                     /gene="uidC"
                     /locus_tag="ACHUU3_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR2083220.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(6652..8160)
                     /locus_tag="ACHUU3_003223"
     CDS             complement(6652..8160)
                     /locus_tag="ACHUU3_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR2083221.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8261..9436)
                     /gene="manA"
                     /locus_tag="ACHUU3_003224"
     CDS             complement(8261..9436)
                     /gene="manA"
                     /locus_tag="ACHUU3_003224"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR2083222.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            9635..11281
                     /gene="fumB"
                     /locus_tag="ACHUU3_003225"
     CDS             9635..11281
                     /gene="fumB"
                     /locus_tag="ACHUU3_003225"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR2083223.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
BASE COUNT         2812 a         2650 c         2916 g         2915 t
ORIGIN      
        1 gacggaaaat gcgccatcat cacaggtgcg ggtgcaggta ttggtaaaga aatcgccatt
       61 acattcgcga cagctggcgc atctgtggtg gtcagtgata ttaacgccga cgcagctaac
      121 catgttgtag acgaaattca acaactgggt ggtcaggcat ttgcctgccg ttgtgatatt
      181 acttccgaac aggaactctc tgcactggca gactttgcta tcagtaagct gggtaaagtt
      241 gatattctgg ttaacaacgc cggtggcggt ggacctaaac cgtttgatat gccaatggcg
      301 gattttcgcc gtgcttatga actgaatgtg ttttcttttt tccatctgtc acaacttgtt
      361 gcgccagaaa tggaaaaaaa tggcggtggc gttattctga ccatcacttc tatggcggca
      421 gaaaataaaa atataaacat gacttcctat gcatcatcta aagctgcagc cagtcatctg
      481 gtcagaaata tggcgtttga cctgggtgaa aaaaatattc gggtaaatgg cattgcgccg
      541 ggggcaatat taaccgatgc cctgaaatcc gttattacac cagaaattga acaaaaaatg
      601 ttacagcaca cgccgatcag acgtctgggc caaccgcaag atattgctaa cgcagcgctg
      661 ttcctttgct cgcctgctgc gagctgggta agcggacaaa ttctcaccgt ctccggtggt
      721 ggggtacagg agctcaatta atacactaac ggaccggtaa acaaccgtgc gtgttgttta
      781 ccgggataaa ctcatcaacg tctctgctaa ataactggca gccaaatcac ggctattggt
      841 taaccaattt cagagtgaaa agtatacgaa tagagtgtgc cttcgcacta ttcaacagca
      901 atgataggcg ctcacctgac aacgcggtaa actagttatt cacgctaact ataatggttt
      961 aatgatggat aacatgcaga ctgaagcaca accgacacgg acccggatcc tcaatgctgc
     1021 cagagagatt ttttcagaaa atggatttca cagtgcctcg atgaaagcca tctgtaaatc
     1081 ttgcgccatt agtcccggga cgctctatca ccatttcatc tccaaagaag ccttgattca
     1141 ggcgattatc ttacaggacc aggagagggc gctggctcgt ttccgggaac cgattaaagg
     1201 gattcatttt gttgactata tggtcgagtc tattgtctct ctcacccatg aagcctttgg
     1261 gcaacgggcg ctggtggtcg aaattatggc ggaagggatg cgtaacccac aggtcgccgc
     1321 catgcttaaa aataagcata tgacgatcac ggaatttgtt gcccagcgga tgcgtgatgc
     1381 ccagcaaaaa ggcgagataa gcccagacat caacacgtca atgacttcac gtttactgct
     1441 ggatctgacc tacggtgtac tggccgatat cgaagcggaa gatctggcgc gtgaagcgtc
     1501 gtttgctcag ggattacgcg cgatgattgg cggtatctta accgcatcct gatcctctca
     1561 tttcgcgggc tggtgataac tgtgcccgcg tttcatactt taatttccct gtacaaaata
     1621 caatctagcc gggatcggag aattcccccc caaaaatatt caccgtagcc atatgtcatg
     1681 agagtttatc gttcccaata tgctcgaacg aacgttcggt tgcttatttt atggcttctg
     1741 tcaacgccgt tttaaagatt aatgcgatca atatcacgct gtgggtattg cagtttttgg
     1801 ttttttgatc gcggtgtcag ttctttttat ttccatttct cttccatggg tttctcacag
     1861 ataactgtgt gcaacacaga attggttaac taatcagatt aaaggttgac cagtattttt
     1921 atcttaatga ggagtccctt atgttacgtc ctgtagaaac cccaacccgt gaaatcaaaa
     1981 aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt
     2041 ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc
     2101 agttcgccga tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct
     2161 ttataccgaa aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt
     2221 acggcaaagt gtgggtcaat aatcaggaag tgatggagca tcagggcggc tatacgccat
     2281 ttgaagccga tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc accgtttgtg
     2341 tgaacaacga actgaactgg cagactatcc cgccgggaat ggtgattacc gacgaaaacg
     2401 gcaagaaaaa gcagtcttac ttccatgatt tctttaacta tgccgggatc catcgcagcg
     2461 taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg
     2521 cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg
     2581 ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt
     2641 tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg
     2701 tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag
     2761 tggcagtgaa gggcgaacag ttcctgatta accacaaacc gttctacttt actggctttg
     2821 gtcgtcatga agatgcggac ttgcgtggca aaggattcga taacgtgctg atggtgcacg
     2881 accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg
     2941 ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg
     3001 ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac
     3061 tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag
     3121 agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac
     3181 cggatacccg tccgcaaggt gcacgggaat atttcgcgcc actggcggaa gcaacgcgta
     3241 aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg
     3301 ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc
     3361 aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg
     3421 agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc
     3481 actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc
     3541 accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt
     3601 ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa gaaggggatc ttcacccgcg
     3661 accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg
     3721 gtgaaaaacc gcagcaggga ggcaaacaat gaatcaacaa ctctcctggc gcaccatcgt
     3781 cggctacagc ctcggtgacg tcgccaataa cttcgccttc gcaatggggg tgctcttcct
     3841 gttgagttac tacaccgacg tcgctggcgt cggtgccgct gcggcgggca ccatgctgtt
     3901 actggtgcgg gtattcgatg ccttcgccga cgtctttgcc ggacgagtgg tggacagtgt
     3961 gaatacccgc tggggaaaat tccgcccgtt tttactcttc ggtactgcgc cgttaatgat
     4021 cttcagcgtg ctggtattct gggtaccgac cgactggagc catagccgca aagtagtgta
     4081 tgcatatttg acctacatgg gcctcgggct ttgctacagc ctggtgaata ttccttatgg
     4141 ttcacttgct accgcgatga cccaacaacc acaatcccgc gcccgtctgg gcgcggctcg
     4201 tgggattgcc gcttcattga cctttgtctg cctggcattt ctgatagggc cgagcattaa
     4261 gaactccagc ccggaagaga tggtgtcggt ataccatttc tggacgattg tgctggcgat
     4321 tgccggaatg gtgctttact tcatctgctt caaatcgacg cgtgagaatg tggtacgtat
     4381 cgttgcgcag ccgtcattga agatcagttt gcaaaccctg aaacggaatc gcccgctgtt
     4441 tatgttgtgc atcggtgcgc tgtgtgtgct gatttcgacc tttgcggtca gcgcctcgtc
     4501 gttgttctac gtgcgctatg tgttaaatga taccgggctg ttcactgtgc tggtactggt
     4561 gcaaaacctg gttggtactg tggcatcggc accgcttgtg ccggggatgg tcgcgaggat
     4621 cggtaaaaag aataccttcc tgattggcgc tttgctggga acctgcggtt atctgctatt
     4681 cttctgggtt tccgtctggt cactgccggt ggcgttggtt gcgttggcca tcgcttcaat
     4741 tggtcagggc gttactatga cagtgatgtg ggcactggaa gctgataccg tagaatacgg
     4801 tgaatacctg accggcgtgc gaattgaagg gctcacctat tcactattct catttacccg
     4861 taaatgcggt caggcaatcg gaggttcaat tcctgccttt attttggggt taagcggata
     4921 tattgccaat caggtgcaaa cgccggaagt tattatgggc atccgcacat caattgcctt
     4981 agtaccttgc ggatttatgc tactggcatt cgttattatc tggttttatc cgcttacgga
     5041 taaaaaattc aaagaaatcg tggttgaaat tgataatcgt aaaaaagtgc agcagcaatt
     5101 aatcagcgat atcactaatt aataatcaat aaaaataatc agaacatcaa aggtgcaact
     5161 atgagaaaaa tagtggccat ggccgttatt tgcctgacgg ctgcctctgg ccttatctct
     5221 gcttatgcgg cgcaactggc tgacgatgag gcgggactac gcatcagact gaaaaacgaa
     5281 ttgcgcaggg cggataagcc cagtgctggc gcgggaagag atatttacgc atgggtacag
     5341 ggaggattgc tcgatttcaa tagtggttat tattccaata ttattggcgt tgaaggcggg
     5401 gcatattatg tttataaatt aggtgctcgt gctgatatga gtacccggtg gtatcttgat
     5461 ggtgataaaa gttttggctt tgccctaggg gcagtaaaaa taaaacccag tgaaaatagc
     5521 ctgcttaaat caggtcgctt cgggacggat tatagttatg gtagcttacc ttatcgtatt
     5581 ccgttaatgg ctggcagttc gcaacgtaca ttaccgacag tttctgaagg agcattaggt
     5641 tattgggctt taacaccaaa tattgatctg tggggaatgt ggcgttcacg agtattttta
     5701 tggactgatt caacaaccgg tattcgtgat gaaggggtgt ataacagcca gacgggaaaa
     5761 tacgataaac atcgcgcacg ttctttttta gccgccagtt ggcatgatga taccagtcgc
     5821 tattctctgg gggcatcggt acagaaagat gtttccaatc agatacaaag tattctcgag
     5881 aaaagcatac cgctcgaccc gaattatacg ttgaaagggg agttgctcgg cttttacgcg
     5941 cagctcgaag gtttaagtcg taataccagc cagcccaatg aaacggcgtt ggttagtgga
     6001 caattgacct ggaatgcgcc gtggggaagc gtatttggca gtggtggtta tttgcgccat
     6061 gcaatgaatg gtgccgtggt ggataccgac attggctatc ccttttcatt aagtcttgat
     6121 cgtaaccgtg aaggaatgca gtcctggcaa ttgggcgtca actatcgttt aacgccgcaa
     6181 tttacactga catttgcaca gattgtgact cgcggctatg aatccagtaa acgagatgtg
     6241 cggattgaag gcgcgggtat cttaggtggt atgaactatc gggtcagcga agggccgtta
     6301 caagggatga atttctttct tgctgccgat aaagggcggg aaaagcgcga tggcagtacg
     6361 ctgggcgatc gcctgaatta ctgggatgtg aaaatgagta ttcagtatga ctttatgctg
     6421 aagtcagaaa taacgccgga gagaaaaacc tccggcgttt cagattgttg acaaagtgcg
     6481 cgttttttat gccggatgcg gcgtaaacgc cttatccagc ctacaaaaac tcataaattc
     6541 aaagtgttgc aggaaaaggt aggcctgata agcgtagcgc atcaggcaat ctctggtttg
     6601 ttttcagatg aaaacgccga agtgaaaatt ctccggcgtt ttggccgtga attactgctg
     6661 cggaattgcc ggtacagccg gaacgttaag agctggcatc gcaaacatac caacgaaatc
     6721 ttccagtggc attttctgcc cgtttaacgt tatctgaccg ttagtatatt gcaggctggt
     6781 ggtgatggta ttgtcctgca aggtggtcag acggaacatc tgccccattg ctgatgcacc
     6841 ttcaacttgc tgtttcgcca gttttttcgc ttgatcttcc tgataacctt ccagcttcgc
     6901 tacctgagtc ataaactcag ttgccatatc caccggaatg gtcagtttcg catccagaga
     6961 tttaaccgaa cgatctactt cctgcgccag cgtttgcggc gcttctttag tcgttgccgg
     7021 atctttcagg aacagcgaca gattcagcgc actttcaccc tgactgtttt tccagcttag
     7081 cggcgcgata gtaatcaccg gatcgccttt caacatcagc ggcagggcgc taaagaaggc
     7141 ttccgtcact ttctcctgat aaagttcggg attgttggca atttctggct gtgccagcag
     7201 cgcctgagtt tgcgcgttat attgctggct aaactgatgc caggcttcac catcaatctg
     7261 gccgactttt aaagtcagct tgccgctgcc cagatcctga ttctgtacct tcaggctgtt
     7321 tagcgagtaa tccagttggc tattgatcgt tttaccgtca ttgaccagat ccgatttacc
     7381 gctgatctcc atgccttcca gcagtgccag ttctttgcct tccactgaaa tggtcatttt
     7441 ttccagtgac agtttttggt ttcctacgcg ctcaccaaaa cttgccagcg tgctggaacc
     7501 gtcggttttc agattattaa aggtcaactg cactttctgg ttgtattcgt taactgcgtc
     7561 tatccgacca ctttgcgcct ccccggaaag ggagatggct ttgccgtctc tgtcagcatt
     7621 taactggaac tcgccgccgc taaaggcgac tttttcatcc ttttgctcgt aattcagtgg
     7681 cttgagcgaa atatcggaac tggaatcacc gctgtaacca atgcgcgagt taatctcaaa
     7741 aggcgtttca ccttttgcca tatcaaacag tggtttgctt acttcgttat taaccagcgt
     7801 ggtttgaatt gatgccatcg acgggatcag gttcagtttt ttaagctggg caagcgggaa
     7861 gggaccatga tcaaccgatt cgttgaagat gacgctctga ccgcttttaa tccacggatt
     7921 ttctttcccg gcaatgggtt tcaccaacag ttgcaactgg ctgctgaata cgccgcgatg
     7981 atagttttga taactcactt ccaggttgga ttcaggagct gtcagtttga gttgcgcgtt
     8041 cgcctgcgcg accatgtctt cgagatgggt ttcaatcttc ttgcctgtat accatgcgcc
     8101 gcctgtccag actacgccta gcgcaacaat gacgcctacc gctaccagcg atttattcat
     8161 aatgattatc cataaaatga aatcaggcgg actggccgcc tgaaggtgtt ataagccttt
     8221 aataagctta gcaagagatg ttaatttttt cagtaagctc ttacagcttg ttgtaaacac
     8281 gcgctaaacg gccgtggcct ttgacagtca ccggtgattc gttggcggca ataaacgctg
     8341 attcacccgg tttaagctgt aactgctgag aacctttcca caacgttgca tcgccttcga
     8401 cgcagaacaa aatggcggca ctctgctggc taatggtggt ttctttatca ctaaggtcat
     8461 gcagcgagaa ggcaaaatca tccactggaa tcgggaagtc cagttctgca ccttgtttca
     8521 ccggctgggt caacaactgg ttagccggtt tggcttcgaa tttcacattg gcaaccagtt
     8581 ccggaatatc aatgtactta ggcgtcagac ccgcacgcag cacgttatcg gagttcgcca
     8641 tcacttccag cgccacgcct tgcaggtaag cgtgcggtgt ttcagcgaac aggaacatcg
     8701 cttcgccagg attcaatttc accacattca gtaacagcgg ggagaacagg ccactgtctt
     8761 ccgggtaaaa ttcagaaatt aaacgaatcg tttgccacgg ttcaccctgc tgactgtcga
     8821 gcgccgattt taaaatcgcc agcgcgcggg atttttcttc accctgcata ttcaacaggc
     8881 tggcgaacag ttcgcttaaa cgttcggcat taggctgttg taaaaagtga gcaattgccg
     8941 gatgtgcacc tgcgactggc tggagtaggg agacaatctc ggaaaactca cgaaacgcgt
     9001 tcatcgcaag gaaaggcgtc agcgcaaaaa ccagctccgg cttgtggtta ggatctttat
     9061 agttacgctc ggcggcatcc atcgggatac ctgcggcatt ttctttggca aaaccgattt
     9121 cagaattgcg tttgtttgga tgaacctgaa tggagagcgg ctgtgctgcg cataatactt
     9181 tgaacaggaa aggcagttcg ccaaagcgtt tggcaacggc ctctccgagc agagtcgatt
     9241 tatcactctc aatcacatca cgcagtgaaa cgatatctcc ggcggcattc tgcactcgtg
     9301 aactgctttt cggatgtgcg cccatccaca gctcggccat cggctggctg gacggatttt
     9361 ccataccata aagttcagtc aacgccgttt tgctgcccca ggcatagttt tgcactgagt
     9421 taatgagttt ttgcatgatc aatccctgtt ttaatgtgga aattaatccc actattaaag
     9481 caagaatcct acgggaagta acctggagcc gcaaaaagtc gtactagtct cagtttttgt
     9541 taaaaaagtg tgtaggatat tgttactcgc ttttaacagg gcaacggaac acccgcccag
     9601 agcataacca aaccaggcag taagtgagag aacaatgtca aacaaaccct ttcattatca
     9661 ggctcctttt ccactcaaaa aagatgatac tgagtattac ctgctaacca gcgaacacgt
     9721 tagcgtatct gaatttgaag ggcaggagat tttgaaagtc gcacccgaag cgttaactct
     9781 gttggcgcgt caggcgtttc atgatgcgtc atttatgctg cgtccggctc accaacaaca
     9841 ggtggccgac attctgcgtg acccggaagc cagcgaaaat gataaatatg tggcgctgca
     9901 attcctgcgc aactccgaca tcgcggcgaa aggtgttctg ccaacctgtc aggataccgg
     9961 cactgcgatt attgttggta aaaaagggca gcgtgtatgg accggtggct gtgatgaagc
    10021 ggcgctggcg cgcggtgtct ataacactta tatcgaagat aatttgcgct actcgcaaaa
    10081 cgcgccgctg gatatgtata aagaggtgaa taccggcacc aatctgccag cgcagatcga
    10141 tctttatgcc gttgatggcg acgagtacaa attcctctgt atcgccaaag gtggtggttc
    10201 ggcaaacaag acgtatctct atcaggaaac caaagcgtta ctaacgccgg ggaaactgaa
    10261 aaattacctg gttgagaaga tgcgcacgct gggtacggcg gcctgtcctc cgtatcatat
    10321 tgcgttcgta attggtggaa cttctgcaga aactaacctt aaaacagtga aactggcttc
    10381 cgctaaatac tatgacgaac tgccaacgga agggaatgag cacggtcagg cattccgcga
    10441 tgtggaactg gaaaaagaat tgctgatcga agcgcaaaat ctcggtctgg gtgcgcagtt
    10501 tggtggtaaa tacttcgctc acgacatacg cgtgattcgc ctgccacgtc acggcgcatc
    10561 ctgtccggtt ggtatgggtg tttcctgctc tgctgaccgt aatatcaaag cgaagatcaa
    10621 ccgtcagggg atctggatcg aaaaactgga acataatcca ggtaaatata tccctgaaga
    10681 gctgcgtaaa gcgggagaag gcgaagcggt gcgcgttgac cttaaccgtc cgatgaaaga
    10741 gatcctcgca cagttgtcgc agtatcccgt ttctacacgc ttatcgctta acggcacgat
    10801 tatcgtcggt cgtgatattg ctcacgccaa actgaaagag cggatggata acggtgaagg
    10861 gctgccgcag tacattaaag atcatccgat ttactacgcg ggtccggcca aaacgccgga
    10921 aggttatgcc tccggttctc ttggcccaac gaccgccgga cggatggatt cttatgtcga
    10981 tcaactgcaa gcgcagggcg gaagtatgat catgctggcg aaaggcaacc gcagccagca
    11041 ggtgacggat gcctgtaaaa aacacggcgg cttctacctt ggcagtatcg gtggtccggc
    11101 cgctgtattg gcgcagggaa gtattaagag cctggaatgt gttgaatatc cggaactggg
    11161 aatggaagcc atctggaaaa ttgaagtgga agatttcccg gcgtttatcc ttgtggatga
    11221 taaaggaaat gacttcttcc agcagataca actcacacag tgcactcgct gtgtgaaata
    11281 aacagagccg ccc
//
LOCUS       DAZPLI010000128        11242 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZPLI010000128 DAZPLI010000000
VERSION     DAZPLI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.129"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..309
                     /locus_tag="ACHUU3_003226"
     CDS             <1..309
                     /locus_tag="ACHUU3_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR2083224.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN
                     "
     gene            508..789
                     /gene="ppiC"
                     /locus_tag="ACHUU3_003227"
     CDS             508..789
                     /gene="ppiC"
                     /locus_tag="ACHUU3_003227"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR2083225.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="ACHUU3_003228"
     CDS             complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="ACHUU3_003228"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR2083226.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2501..3394
                     /gene="ilvY"
                     /locus_tag="ACHUU3_003229"
     CDS             2501..3394
                     /gene="ilvY"
                     /locus_tag="ACHUU3_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR2083227.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="ACHUU3_003230"
     CDS             complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="ACHUU3_003230"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR2083228.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="ACHUU3_003231"
     CDS             complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="ACHUU3_003231"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR2083229.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="ACHUU3_003232"
     CDS             complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="ACHUU3_003232"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR2083230.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="ACHUU3_003233"
     CDS             complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="ACHUU3_003233"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR2083231.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8117..9763)
                     /gene="ilvG"
                     /locus_tag="ACHUU3_003234"
     CDS             complement(8117..9763)
                     /gene="ilvG"
                     /locus_tag="ACHUU3_003234"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR2083232.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9766..9816)
                     /gene="ilvX"
                     /locus_tag="ACHUU3_003235"
     CDS             complement(9766..9816)
                     /gene="ilvX"
                     /locus_tag="ACHUU3_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR2083233.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="ACHUU3_003236"
     CDS             complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="ACHUU3_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR2083234.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10354..>11242
                     /locus_tag="ACHUU3_003237"
     CDS             10354..>11242
                     /locus_tag="ACHUU3_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFR2083235.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGRHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNG"
BASE COUNT         2555 a         3090 c         2950 g         2647 t
ORIGIN      
        1 gtcgtgctca acggaaaaat taatggcaac gcatgtatcg tcgttccatt atcaaccact
       61 cgcgatcatg acaagttaaa tcgtggaatg catgttgaaa tagccagtaa tgttatcaat
      121 gacctgcaat tctttgatca gcagattcgt tgggccaaag ccgacctagt acaacaagtc
      181 agtcgaaacc gattaaacag agcaaggact tacagagggt atcttaatca atgtttgcca
      241 catgagttgg ttgcagacat acagagggcg gtcatcaagt caatcaatgc catatcgctc
      301 ataaattgac aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa
      361 gttttctccc cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta
      421 ccagcccctc cctgactaac aattacgtta gcatctgcgg atcatgctaa aatcgccgcc
      481 tgacaattat tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt
      541 gtaaaagaag agaaactggc tctggatctt ctcgagcaga ttaagaacgg tgccgatttc
      601 ggcaagctgg cgaagaaaca ctccatttgc ccatcaggca aacgcggcgg tgatttaggt
      661 gaattccgcc agggtcagat ggttccggcg tttgataaag ttgtgttctc ttgtccggta
      721 ctggagccaa ccggcccgct gcacacccag ttcggttacc acatcattaa ggtgctgtac
      781 cgtaactaat agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg
      841 tgggagaggg tcggggtgag ggcatcagcg cgcacttaac ccgcaactgc aatacgtttc
      901 atatccgtca tatagccgcg cagtttctta cctacctgct caatcgcatg gcagcgaatc
      961 gcttcgttca catcacgcag ttgcgcgtta tctaccgcac cttccggaat agctttaccc
     1021 agatcgcccg gttgcagctc tgccataaac ggtttcagca gcggcacaca agcgtaagag
     1081 aacagatagt taccgtactc ggcggtatca gagataacca cgttcatttc gtacagacgc
     1141 ttacgggcga tggtgttggc aatcagcggc agttcgtgca gtgattcata gtaagcagat
     1201 tcttcgatga tgccggaatc aaccatggtt tcaaacgcca gctcaacgcc cgctttcacc
     1261 atcgcgatca tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac
     1321 tgcggcgcgg tttcgaaggc ggttttgccg gtctcttcac gccaggtcag cagtttctta
     1381 tcatcgttgg cccagtccgc catcataccg gaggagaatt cgccggagat gatgtcgtcc
     1441 atatgtttct ggaacagggg tgccatgatc tctttcagct gttcagacag cgcgtaagca
     1501 cgcagtttcg ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc
     1561 gcttcggtga tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg
     1621 ccctcttcca ccagcttgtc gaagcacagc agagagccag cctgcaacat accgcacagg
     1681 atggtttgct cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg
     1741 cccgcacggt gaccaccggt tgctgccgcc catgctttag cgatcgccat gccttcgcct
     1801 ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac
     1861 tcttcacgta cttcggtgcc agggcatttc ggcgcaacca tgactacggt aatgtcttta
     1921 cggatctgct cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg
     1981 tctttcatca gtggctgtac ggtgcgcact acatcagagt gctgcttgtc tggcgtcagg
     2041 ttaaccacca gatccgcctg cgggatcagt tcttcgtaag taccaacttt aaaaccgttt
     2101 tcggtcgctt tacgccagga cgcgcgcttc tcggcaatcg cttctttacg cagagcgtag
     2161 gagatatcga gaccagaatc acgcatgttc aggccctggt tcagcccctg tgcgccacag
     2221 ccgacgataa ccactttttt accctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc
     2281 cccataaagc gacatttgcc cagctgtgcc agctgctggc gcaggttcag tgtattgaag
     2341 tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg
     2401 agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca
     2461 cattgcaatt tctgcaacgt caacatcgag ggctgcgcca ttggatttac gcgatctgaa
     2521 aaccttcctg catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt
     2581 tagcccatcc acgctctcac ggcagattca gcgcctggaa gaagatcttg gtcagccgct
     2641 atttgtgcgc gataaccgca cggtaacgct gacggaagcg ggcgaagagc tgcgcgtttt
     2701 cgcccagcaa acgctgttgc agtatcagca attgcgccac accatcgatc agcaagggcc
     2761 gtcgctctct ggcgaattac atatcttctg ctcggtgacc gctgcctaca gccatctgcc
     2821 gccgattctg gatcgcttcc gcgcggaaca cccgtcggtg gagatcaaac tcaccactgg
     2881 tgatgcggca gatgcaatgg aaaaagtggt cacgggagaa gcggatctgg cgattgccgg
     2941 gaaaccggag acgctacccg gcgctgtggc gttttcgatg ctggagaatc tggcagtagt
     3001 gctgattgcc cccgcgctgc cctgcccggt gcgtaatcag gtatctgtcg aaaagcccga
     3061 ctggtcaacg gtgccgttta ttatggccga tcaggggccg gtacgccgcc gcattgaact
     3121 gtggtttcga cgcaataaaa tcagcaaccc gatgatttac gccacggttg gcgggcatga
     3181 agcgatggta tcgatggtgg cgctcggctg tggtgtggcg ctgttgccgg aagtggtgct
     3241 ggaaaacagc ccggagcctg tacgcaaccg ggtaatgatt ttagatcgca gcgatgagaa
     3301 aacgccgttt gaactgggcg tatgtgcaca aaaaaagcgg ctgcatgagc cgctgattga
     3361 ggcgttctgg aggattttgc cgaaccacaa atgacgttgt cgcgcgggta ggcctgataa
     3421 gcgaagcgct atcaggcatt tttccctaac ccgccaaaaa gaacctgaac gccggattat
     3481 tggtttcgtc gtggcaatcg tagcccagct catttagccg ggtttcgaaa tccggttcat
     3541 ggtcgccgag ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt
     3601 ggaacaaaga aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg
     3661 gtgattccgg gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc
     3721 cgaccatata gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt
     3781 agccgccgtc gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc
     3841 gcacgccgac aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga
     3901 ccgatcgccc gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg
     3961 tcaccgccaa caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca
     4021 ggccgtggaa gttcacgttg gcaccggaaa gaatatgcgc caggcgttcg ccgcgaatgt
     4081 tgtgctgagc gatatatttt ttcattcccg ccagcgccaa cgcgccagag ggttccgcca
     4141 ctgcgcgcac atcttcgaat aaatccttca tcgccgcaca gatcgcatca ctatcgacgg
     4201 tgatgatgtc gtcgagatac tcctggcata aacggaaggt ttcgtcgccg atgcgtttta
     4261 ccgcgacacc ttcggcgaac agtcccacgc gcggcagatc aaccggatga cccgcatcca
     4321 gcgctgcttt caggcaggcg gagtcttccg cttctacggc gatcactttg atttgtggca
     4381 tcagttgttt gatcagcacc gcaacgcccg ccgccagacc gccgccgccg actggcacaa
     4441 atacgcggtc gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg
     4501 caatcaccat cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt
     4561 cgatcgcttt agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga
     4621 atccacgtac cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct
     4681 tcacgcctaa ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag
     4741 tgatcacgcc gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc
     4801 gcagcttaaa gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa
     4861 gacgcgacga cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg
     4921 gcgcgcgcag cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg
     4981 agtcagccat tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct
     5041 ggttgccagg ctggcgtagg cgcgcagggc aaaggaaacc tgacgttcac ggtttttcgg
     5101 cgtccaggct ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc
     5161 gcttacctgt aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc
     5221 aatcagacca atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc
     5281 agaggtgccg ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat
     5341 tgatttcagg aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc
     5401 ttcatagcgg atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac
     5461 cgcgtcgtcc tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc
     5521 gacgcccgct gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag
     5581 gccgccgtct ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc
     5641 atcgagagaa tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg
     5701 gcctgcgcgg aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc
     5761 cagagtttgc ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc
     5821 gcgatccagt tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg
     5881 gtatttttgg gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc
     5941 gatatcactc atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac
     6001 cgtgttggta gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt
     6061 gctagcaata ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa
     6121 ttcaacaatg cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag
     6181 cagcgagccg ttgcctggct gcgacaaacc cagcgcttcg gtcaggcagt tcattgagtt
     6241 agcggtaaac atcccggagc aggaaccgca ggttgggcag gcggaacgtt caacctgatc
     6301 gctctgggag tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag
     6361 cttgatgatc tgctcggaca gtttggtttt cccggcttcc atcgggccgc cggaaacaaa
     6421 gatcaccgga atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca
     6481 gttagagatg cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc
     6541 agcgatcagt tcgcgagatg gcagtgaata aagcatcccc ccgtggccca tagcaatccc
     6601 atcatccacc gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc
     6661 ggcgaccagt ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga
     6721 gttcacaacc gcgataatcg gcttgccaaa atcggcatcg gtcattccgg tggcgcgcca
     6781 cagcgcacga gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg
     6841 catactttat ttactcccag tgtctgtctc gtaaatggga cggtaagtgc cgtcccattt
     6901 tttgtattta ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga
     6961 agaggccgaa gaaggcttgc tgaatgcatt tggtaaccgg gccgcaacgg ccttcaccca
     7021 cctgaatacc gtctacactg cgcactggcg tgatttctgc cgccgtaccg gacataaaca
     7081 cttcatccgc caggtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca
     7141 gctctttcgc cagtttgatg atggcatcac gggtaatacc cggcagcgcg gatgaggtaa
     7201 acggtggggt gaacagcacg ccgtctttca cttcaaacag gttttcacct gcgccttcag
     7261 agatgtaacc gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc
     7321 tacccaccag cagggaagag aggtagttac caccggcttt tgccgccgtc gggatggtgt
     7381 ttggtgctgc gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg
     7441 cgcccagata cgctccccac gggaaagcag cgataatcac gtcggttgag tatcccgctg
     7501 gcgggtttac tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg
     7561 tgagattatt tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgcg
     7621 aaaccgggaa gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc
     7681 ggaatacaac cggtcctttg tgcgagtcgt agcaacggat gccttcaaaa accgaagtgc
     7741 catagtgcag cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc
     7801 cattgaacca aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag
     7861 gcgcggattt gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc
     7921 agtttattta actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg
     7981 atatttatat tttgtgcatc gctggcggcg gccatattca ttgagcagac gtggaaacca
     8041 cgatgacgca ccacgcgtaa aacacgttct aaggtttctg gattgaagcg agccgataca
     8101 ttgacctgat gttgcatcat gataatttct ccaacatttc tgaattactg gcgccaggcg
     8161 gcaccagcgg ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat
     8221 cactgttcag catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc
     8281 cagggatgcc gaaggcgctg gctaacatga ggaaatcggg gttatcagta agggtggttt
     8341 cgctgtatcg ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt
     8401 tatcgagtaa gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca
     8461 cattcatcat gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga
     8521 cttgtgcgcc aaccgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga
     8581 tgaaattttc cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca
     8641 catctgtggt cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg
     8701 gcgcgtagat agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg
     8761 cgcagtattg ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat
     8821 ttaaatcacc ctgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga
     8881 tatccatatg gataacactg gcgtgtggtg cgaaagtatt cagtttgccg gtcacccggt
     8941 catcaaaacg tgcgcccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg
     9001 ctttgatgcc gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc
     9061 ccagcccttt cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag
     9121 ccggaactgc ctgcgccata cccacgccac cgccaacgta cagcatcggt ttttgcgctt
     9181 ttgccagcat ctggcgcgct tgctcaactt cggcatgtgg gaaagtcact tcgttttcaa
     9241 cggtggtgaa ccacggttcc aggtcaccgc tggctaactg gatatctttt gggatatcga
     9301 ccagaaccgg accaggacga cctgagcagg caacgtcgaa tgcttcagcc atgatgcgcg
     9361 gcaactcttc cagcgactgc accagaaagc tgtgcttggt acaggctaac gacaatccta
     9421 ggacatccac ttcctgaaat gcgtcagtgc cgataaacgg tgcggacact tgaccggtga
     9481 tggcaacaac agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg
     9541 cgcccggacc agacgtggcg atacatacgc cagttttgcc ggtagcacgg gcataaccga
     9601 tagccgccat tgccgcaccc tgctcatgtc ggcatagcaa gtgctccacg ccgccgtcat
     9661 acaatgcatc gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac
     9721 cctgtgcccg caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct
     9781 gaatcttgag aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt
     9841 aaggtcaaaa aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat
     9901 ctctaagcct ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg
     9961 ctaatcacga ctaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct
    10021 tgttcgaagg aatgcctaaa gagttaccat agattttgcg aatagcacaa gatatttttg
    10081 ggcctatttt tatgatagct ttaataattt caatagaagt tgttgttttt taaaaagaaa
    10141 atagtgagtg aaaaaaattc aatttcagta taaatgttgt tcccttcctc gtagttcatc
    10201 ctttgtccgc tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg
    10261 cacaaataaa caggaatcag ccactaaaat tcacgaatta gcgggctaca gcagcgatat
    10321 cttatgcaca taatggtgac tcaaggaggg cttatgtcac tgtcaattgt tcatacccgc
    10381 gcagccctgg gagtaaatgc gcccccgatc actgttgagg tacatatcag taaaggtcta
    10441 cccggcttaa cgatggtggg cttaccagaa acaactgtaa aagaagctcg cgatcgcgtg
    10501 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt
    10561 gctccagccg atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg
    10621 ctggcggcct cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa
    10681 ctggctctta caggcgctct gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct
    10741 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
    10801 aacggtgaag gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt
    10861 aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat
    10921 ctcagtgatg ttgtcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc
    10981 aggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
    11041 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta
    11101 agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct
    11161 catcacagtg cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa
    11221 atttcgttgg cgcataacgg cg
//
LOCUS       DAZPLI010000129        11135 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZPLI010000129 DAZPLI010000000
VERSION     DAZPLI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11135
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.130"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..428
                     /locus_tag="ACHUU3_003238"
     CDS             <1..428
                     /locus_tag="ACHUU3_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR2083236.1"
                     /translation="FHDCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIY
                     NMSAGNNVKVAADAFLEIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDKSIEIL
                     LLSCFAASDQKAIQRYIPQSQDGEDFRSHLQYKLPLSAH"
     gene            639..962
                     /locus_tag="ACHUU3_003239"
     CDS             639..962
                     /locus_tag="ACHUU3_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR2083237.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            959..1789
                     /gene="amiD"
                     /locus_tag="ACHUU3_003240"
     CDS             959..1789
                     /gene="amiD"
                     /locus_tag="ACHUU3_003240"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR2083238.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1786..2799)
                     /locus_tag="ACHUU3_003241"
     CDS             complement(1786..2799)
                     /locus_tag="ACHUU3_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR2083239.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2898..4328)
                     /locus_tag="ACHUU3_003242"
     CDS             complement(2898..4328)
                     /locus_tag="ACHUU3_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR2083240.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(4339..5340)
                     /gene="ltaE"
                     /locus_tag="ACHUU3_003243"
     CDS             complement(4339..5340)
                     /gene="ltaE"
                     /locus_tag="ACHUU3_003243"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR2083241.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5377..7095)
                     /gene="poxB"
                     /locus_tag="ACHUU3_003244"
     CDS             complement(5377..7095)
                     /gene="poxB"
                     /locus_tag="ACHUU3_003244"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR2083242.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(7228..8196)
                     /gene="hcr"
                     /locus_tag="ACHUU3_003245"
     CDS             complement(7228..8196)
                     /gene="hcr"
                     /locus_tag="ACHUU3_003245"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR2083243.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(8208..9860)
                     /gene="hcp"
                     /locus_tag="ACHUU3_003246"
                     /gene_synonym="priS"
     CDS             complement(8208..9860)
                     /gene="hcp"
                     /locus_tag="ACHUU3_003246"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR2083244.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(10004..10903)
                     /gene="lysO"
                     /locus_tag="ACHUU3_003247"
     CDS             complement(10004..10903)
                     /gene="lysO"
                     /locus_tag="ACHUU3_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR2083245.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         2710 a         2927 c         2941 g         2557 t
ORIGIN      
        1 tttttcatga ctgtagtatg catggtgtca agataaaacc atggttgcca gtaaaatgga
       61 caaaagagtt aataaatgat tatttgtatg gctgtttact atctctgtac tctatctgtg
      121 caagggatat atataatatg agcgccggga ataacgtgaa ggttgctgcg gatgctttcc
      181 ttgaaattat tttctctctt aagaataaat attgcattaa actattaagc gctcaggaac
      241 gggcgtttat ttacgaattt gcaagaatga tcttcgctta tattaatgac aaaagcattg
      301 agatcttatt actttcttgt tttgcggcgt ctgaccaaaa agcaatccag aggtatatac
      361 ctcagtcaca agatggggaa gactttagga gtcatttaca atataagttg cctctttccg
      421 ctcattaatg ttgttggtgc aacataataa gacaggtaag cggtacccta taatgaaacc
      481 aatatcgggt tatgcaacgg ataatcgtaa agacttaagt gggtgttttt gacacaatga
      541 gatgatttcc taaactgatc aaaaccttct tcccaacatg gcatatcagt tcgataatgc
      601 tttagagatc gaaggcttaa caaacaagga gatcgatcat gcaattttct acaaccccaa
      661 ctctggaagg ccagaccatc gttgaatatt gcggtgtggt gaccggcgaa gcgattttag
      721 gtgccaatat tttccgtgat ttctttgccg gtatccgcga tatcgttggc ggacgttccg
      781 gtgcgtatga aaaagaactg cgtaaagcac gggagatcgc ctttgaggaa ttaggctccc
      841 aggcgcgggc gctgggggcc gatgccgtcg tcggtattga tatcgactac gaaacggtcg
      901 ggcaaaacgg cagtatgctg atggttagcg tcagcggtac ggcggtgaaa acgcgtcgat
      961 gagaagagtc ttctggctgg tcgctgccgc tctgttattg gcagggtgtg caggcgaaaa
     1021 aggcattgtc gagaaagagg gatatcagct tgatacccga cgccaggcgc aggcggcgta
     1081 tccgcgcatt aaagtgctgg tgatccacta caccgcagat gattttgata gctcgctggc
     1141 gacactgacc gataagcagg tcagctcgca ttatctggtc cctgcggtac caccgcgata
     1201 caacggtaaa ccgcgcatct ggcaactggt gccggaacaa gaactggcct ggcatgcggg
     1261 gattagcgcc tggcgcgggg caacgcgcct taacgacacc tctattggca ttgagctgga
     1321 aaaccgtggc tggcaaaaat cggccggagt gaaatatttt gccccgtttg aaccgacaca
     1381 gattcaggcg cttattccac tggcgaaaga tattattgcc cgttatcaca tcaagccgga
     1441 aaacgtagtg gcacatgcgg atatcgcacc gcagcgcaaa gacgatccgg ggccattatt
     1501 tccctggcag caactggcgc agcaggggat tggtgcctgg ccggatgcgc agcgggttaa
     1561 cttttacctt gccgggcgcg cgccgcacac accggtagat accgcgtcat tgctggagct
     1621 tttggcgcgc tacggttatg acgttaaacc tgatatgaca ccgcgcgagc agcggcgcgt
     1681 gattatggca ttccagatgc atttccgccc gacgttatat aacggcgaag cggatgcaga
     1741 aactcaggcg attgccgaag cattgctgga gaaatacggg caggattagc gcggcagttt
     1801 tccgtggtcg cgtagccagg cggcagtttt ctcgatacct tcatctaggg tgatgatcgg
     1861 ctgataacct aactcttcct gcgcacgcgt aatatccagc gtaaagtcaa aattcaactt
     1921 ggagacgccg tagtgggtca gcggcggctc ttttgctgac ttgcggccta aacgctccat
     1981 gctgcgggcg atcatatcca gcatcgggta ggggacggaa cgaatacgac agtcaatatt
     2041 caactcgtcg atcagcttct gcacgatgct gcgcagtgtg cgatgctcgc cgttggtgat
     2101 attgtacaca cgcccggaag gtagcttatc gcaggcttcc tggcttgcca gccacattgc
     2161 gtgcacggta ttttcatagt aggtcatatc gaccagcgca ctgccgccat gcggtaacag
     2221 aatactgccg tagtggtgca tcatatgcgc cagacgggga ataaagactt tatcgtgcgg
     2281 tccgaacaga ctttgtgggc gcagaatagt aaagcgcgtt tgtggattcg cctgcgaaag
     2341 catattgatc acttcttcgc tggctgcttt gctgcgggca aactcgttgg cgaagcggtg
     2401 agggcgaaaa tcttctttaa tatcgcgatg gtggtgataa tcgaagtaca gggaggggga
     2461 agagatatga ataaagttac gcacacccca ggcgacagcc cattcaccca ggcggcgagt
     2521 ggcgcgaacg ttagccagat cgaaagcctg ttgtgtcccc cagggtgagg taaagctgga
     2581 gcagtgccac agcgtatcaa tgcccgcgag catcacttta gcttgtgatg aaaccagctc
     2641 ggtcagatcc gccggaacaa actctgcgcc cattttttcc agcaatttgc ccattgcctc
     2701 gttgcgaccg gtcgctcgca cgctgatgcc tttctggcat aaaaactcta ccgcgtttcg
     2761 acctaagccg ctggtggcgc cggtaaccag taccttcata tcaatccact gttgttgaga
     2821 aaatgacgtg cgcattcttc cgtgatttcc cccatgatgc aatgggaaac atgaaagaat
     2881 aacgcaggtt ttgtcgatta atctgtgctt tgttctgcca gtctggcgat ttgttttgcc
     2941 attccgcgaa aaataaacag atgcgcgggg atcatcaata accagtaaaa cagccccggc
     3001 ataccgtgcg gatgccagaa agcgcggaca tcgatagtac gatagtcgcc tttatcttcc
     3061 aggctaaaac acagtcgtcc cagccccgac gctttcatgc caaataacaa cgtaagttgt
     3121 ttttccggtt caacgacaat cactttccag ctatccaccg catcgccagt ctgtaaatat
     3181 tcgcgctccg ggcggccttt cgccagctta tgaccgatcg cgcggtccat caacgcccgt
     3241 gtctgccaca aaatattgcc aaagaaataa cgctctttac cgccgatttg gttcactacc
     3301 tgccataaag cagcaaggct ggcggacgtt ttaacggtaa accccgcctg tttggcaaaa
     3361 taaccgtact ccggtcgcca gcgggcaaag gcctgagcgt cgtagcccca gtcgctggag
     3421 ttgaccagtt tttcctcctc tttcaacgtg ctacgtaccg cgtcatcgaa agcgatcagc
     3481 cgttgtggga tgagtgcacg tagcgcggta tcatccgcca gcagatcgtg tttcagcccc
     3541 tgaatcaacg ccctggcggt ggtgggcggt acggaagtaa tcacattgag aaaccacacc
     3601 gaaatccagc gggtggggag ggggatgggg atcaaccagc ggcgcttacc gctcaccgcc
     3661 ataaaatgtt caaactgttg ctgataactg agcacctctg gtccggcggc ttcgaagatg
     3721 cggtgttcgc tggccggatg atctaacaac gccaccagat agtgcagcaa gttttccagc
     3781 gcgatgggcg tggtgcgtga acgtacccag cgtggcggcg ttaacactgg caggttgtag
     3841 accatatcgc gcatgacttc gaacgccgct gaacctgcgc caacgataat tccggcccga
     3901 agttcggtca caggtacatt cgcttcacga agaatgtccg ccgtagcctg acgagcacgc
     3961 agatgatccg actgctcatg tggcggggcc tgcaacgaac tgagaaagat taattgctta
     4021 actggtactt cacgtagcgc atcgcggacg ttgagagcca cctggcgctc ctgagcgata
     4081 aaatcgccgc cttcgcccat gctgtgcacc agaaaataga cggtatcgat atcctgcaac
     4141 agggccggaa ggttatccgg ccagctgaga tcgactttat ggcaactgac gtttgccagt
     4201 tgcagctttg caagcctgtc gacatgacgt gccgccgcca ggatctgatg cccttgctgg
     4261 ctgagtgtgc gcaccagatg ctgaccaatg tagccactgg caccgagaac taaaatgcgt
     4321 tgcggcacgt ctctctcctt aacgcgccag gaatgcgcgc cagtgggcgg cgacttccgc
     4381 cagttgttcg cgcgagacgt caagatgcgt caccaggcgg acaatcggcg aggcgttaat
     4441 cagcacgttt ctcgctttca tgtattcgcc taacgcggca gcattttctt ccccgacgcg
     4501 aacaaacagc atattagtgt cctgacgcat cacatccgcg cctgcttcac gcagttgctc
     4561 cgccatccag gcggcgttgt cgtgatcttc ctgcaaccgt gcgacgttat ttttcagggc
     4621 atacatcccg gcggcagcca gaatcccgga ctgacgcatc ccgccaccgg ccattttccg
     4681 ccagcggatg gcacgtttaa tgtaatcacg attaccgacg agtaatgaac cgactggcgt
     4741 cccaagacct ttcgacaggc aaatggtgaa cgaatcacaa tattgcgtga tctctttcag
     4801 ttcgcagccg taagccacca cggcattaaa gatgcgcgca ccgtcaacat gcagcgccag
     4861 attgcgcttg cgggtaaatt cccatgcttc tttcaggtat tcacgcggca gcactttgcc
     4921 gttgtgggtg ttttctagac tgagtaattt ggtgcgggcg aaatggatat cgtcgggttt
     4981 gattttcatc gccactttat ccagcggtag cgtgccgtcg gcagccgcgt cgatgggttg
     5041 cggttgaata ctgcccagca ccgctgcgcc accggcttca aacagataat tatgcgcggc
     5101 ctgaccgaca atatactctt cgccgcgttc gcagtgactg agcagagcga ccaggttagc
     5161 ctgggtgccg gtcggcagaa aaatggcggc ttctttaccg gaaagctctg cggcgtagtc
     5221 ctgcagagca ttaacggtag ggtcgtctcc gtaaacgtcg tccccaaccg gggcggccat
     5281 cattgcttcg agcatggcgc ggctcggtcg ggtaacggta tcactgcgta aatcaatcat
     5341 ggcatgtcct tattatgacg ggaaatgcca ccctttttac cttagccagt tcgttttcgc
     5401 cagttcgatc acttcatcgc cgcgtccgct gatgattgcg cgcaacatat acaagctaaa
     5461 tcctttggcc tgttcgagtt tgatctgcgg tggaatggct aactcttctt tggcgaccac
     5521 cacatccacc agcaccggac cgtcgatgga gaaggcgcgt tgcagggctt catcaacttc
     5581 agacgctttt tctacacgga tacccgtaat gccgcacgct tcggcaatgc gggcaaagtt
     5641 tgtgtcgtgt agttcggtgc cgtcagtcaa atagccacca gctttcatct ccatcgccac
     5701 aaagcccagc acgctgttgt taaagacgac aattttcact ggcagtttca tctgcactac
     5761 tgagaggaaa tcgcccatca acatgctaaa accgccatcg ccgcacatgg cgaccacctg
     5821 acgttctggc tctgtcgcct gcgcacccag cgcctgcggc atggcgttag ccatcgaacc
     5881 gtggttaaac gaacctaaca ggcgacgctt gccgttcatt tttagataac gtgccgccca
     5941 caccgttggc gtaccaacgt cacaggtgaa aatagcgtca tcggcggcaa aatgactaat
     6001 ttgctgcgcc agatattgcg ggtgaatggc tttctcgctc ggtttagcta aatcgtccag
     6061 ccctttgcgg gcgtcgcggt aatcttccag cgctttatcc agaaacttgc gatcggcttt
     6121 ttcttccacc aatggaagca atgcacgcag agtcgacttg atatcgccga ccagtgccat
     6181 atccaccttg ctgtgagcgc cgatgctggc tgggttgata tcaatctgaa tgattttggc
     6241 atcggtcggg tagaaggcgc ggtagggaaa ttgcgtgccg agtagcacta acgtgtcggc
     6301 gttcatcatg gtatggaaac ctgacgagaa gccgattaac ccggtcattc caacatcata
     6361 cggattatcg tattcgacat gttctttacc gcgcagggca tgaacaatag gcgctttaat
     6421 tttcccggca aactcaacta actctttatg cgcccccgcg cagccgctgc cacacatcag
     6481 ggcgatattg ctggaataac gcagcagttg cgccagtttg cgtaactctt cttcttccgg
     6541 cgtcacgact ggttgtggcg catgatacca gtgcgtggtt gccccttctg gcgcaggttt
     6601 taacgccacg tcgcctggta acacgacaac cgaaacgcca cggttaagca ccgctttgcg
     6661 catggcaatc gccagtactt gtgggatctg ctccgggctg gaaaccagct cgcaatagtg
     6721 actacattcg cggaatagct cttgtgggtg ggtttcctgg aaatagccgc tgccaatttc
     6781 gctggaggga atatgagcgg caatcgccag taccggaacg tgattgcggt ggcaatcgaa
     6841 caggccgttg attaagtgca ggttgccggg gccgcacgat ccggcgcaga ccgccagttc
     6901 tccgctaagt tgtgcttcag cgccagcggc aaaggccgcc acttcttcgt ggcgggtgga
     6961 catccactcg atggtgccca tgcgattaag actgtcacta agaccgttca gagagtcgcc
     7021 tgtgactccc cagatgcgtt tcacccctgc cgattcgagt gttttggcga tataagctgc
     7081 aaccgtttgt ttcatggttc tccatctcct gaatgtgata acggtaacaa gtttagttca
     7141 tctgacggag ggggaaggga tgggagagaa aggaggcact aacggttaaa tagcccgatg
     7201 aaaggaatat catcgggcat aaggcgatta tgcgagaacc aaatccccct gcggatggca
     7261 ggagcaggcc agtacgtaac cttcagcgat ttcggcgtcg gtcagcgtca ttgtactgct
     7321 caccgtatat tcaccggaaa ccacttttgt cttacagcag ccgcaaacac ccgcacggca
     7381 ggcagcgaca accggaacgt tattgctttc cagcgcctcc agtagcgtgg tgccaaccgg
     7441 ggcgtaaaat tctcgtgccg gttgcagttt ggtgaatttc agaccgctgg tcgccgcttc
     7501 tgctactggg gtgaagaatt tctctttaaa gaaacgcgtc acgccgagcg ctttcacttc
     7561 ctgctctacc caatccatat acggagccgg gccgcaggtc atcacggtac gtgaagctaa
     7621 gtcaggtaca cctgccagca gttcgcgagt gagacgacca gcgataaagc cttcggtaac
     7681 gttattttct gccaccagcg ttaccggata gttacgccac tcatcggcga aaataacatc
     7741 ctgcggcgta cgcacgttgt agatcacccg cacatcggcc tgtggacggt tcttcgcaag
     7801 ccagcgacgc atcgacataa tcggcgtgac gccgcagcct gccgccagca acaggaattt
     7861 atcttctgct ttatcgtcgc aggtaaattc ccccatcgcg tccgaaagcc agagataatc
     7921 accgcgtttt acatcgcgcg tcagccactg ggagccgaca ccgtcatcaa tccgccgcac
     7981 ggtcagggtg atatattcac tcacgcctgg cgtggaggaa atggtgtaag cacgcagcgt
     8041 ttccgctgag tttcgcacgc tgaccagtgc atattgcccg gcgcgatatg ggtagtaatc
     8101 gtggcaaatc agggaaatcg tccacacatc cggcgtttct tgcgtaatgt gatgaacctg
     8161 catccgccac gggcattgat tcgttggcat cgtcatcgac aaactcctta cgcgctcaac
     8221 agttgcttca tgtcttcttc aacagtggtg atagaacgca ggccgaattt ctcgttcagc
     8281 accgccagca ggtctggtgt caggaaacca ggtgcagtcg ggccggtgac gatatttttc
     8341 acgcccagag aaagcagcgt cagcagaatg acgatcgctt tctgttcaaa ccaggagagc
     8401 accagcgaca gcggcagatc gttcacacca cagcccagtt tctctgccag agtgacagcc
     8461 agaataatcg ctgagtaagc atcgttacat tgacctgcat ctaccagacg cggcagacct
     8521 tcgatatcgc caaactcaag tttgttaaag cgatatttac cacaggcgag ggtcaggatc
     8581 aggcagtcat ccggcacgct ggtggcgaaa tcggtgaagt agtggcgctc gccgcgtgcg
     8641 ccgtcacagc caccaagcag gaagatatga cgcagttttt cacggctcac cagatcaatc
     8701 agcgtatcag cagcgccaag cagcgtctgg cgaccaaaac ccacggtgat aaggtgcgga
     8761 atttcgctgt acgggaagcc tgccatctgt tgcgcctggg tgataaccgc agagaaatca
     8821 tcaccgtcca gatgacgcac gccaggccag ccaacaatgc tgcgggtcca gatacgatcg
     8881 tcataagcgc ctacggttgg gtcgatgatg cagttcgagg tcatcacgat ggggccaggg
     8941 aaacgagcga actccacttg ctgattctgc cagccgctgc cgtagttacc gaccagatgc
     9001 ttgaatttac gcagctccgg gtagccatgc gcaggcagca tttcgccgtg ggtgtagaca
     9061 ttaacgcccg tgccttcggt ctgttccagc aggttgtaga gatctttgag atcgtgaccg
     9121 gaaatcagaa tgcatttacc cgccgtcgct ttgacgttga cctgggttgg cgtcgggtga
     9181 ccgtatttac cggtttcgcc tgcatccaga atgctcatca ctttgaagtt catctggccg
     9241 atttccattg aacactcaag aagcgcgttc atatcggcag gccaggtccc cagccacgcc
     9301 atgattttat ggtactgggc ataaatatcg ttgtcgtatt gaccgagaac atgcgcgtgt
     9361 tccatatagg ccgccgcacc tttcaggcca tacaggcaca gcagacgcag gccgagaatg
     9421 ttttcgccaa tcgccgcttt atctttgtta ggggtaaatt ctgctgcctg acgttgcagc
     9481 tcgccgagat catcgctcac cagttgcagg tcagccatcg ggttatcgac gcgcgcgttg
     9541 gcatctacag ccaggcattg tgctttcagc gcctcgcgca aggcaatcgc ttcacgagcg
     9601 tagccgacaa tacgcggaga atcgaagtta acgttggtca gggttgagaa aaaggcacgt
     9661 ggcgcgaagc tgtcaacatc gtggttgatg atgccgtatt cacacgcttt taccgcccag
     9721 gcagaaagcc cttgcagcgc cgcgatgagt aaatcctgaa ggtcagaagt ttccgccgtt
     9781 ttaccacaca tcccctgcgc gtatgagcag ccgtttcctg ccggagtacg gatagtttgt
     9841 tcacattgca cacaaaacat gatcacacct tttaaagtta tatttaatat acatgtttaa
     9901 ggttaagacg cttaacgcgg ggataaaagg gatttttcat gcaactttaa gggagattga
     9961 tttagcgcaa ttttggcggc agggatctac cgccagagag gtattacgca gagaaaaagg
    10021 cgatgaggat cggcactaac aggctaagaa taaaaccgtg aacaattgcc gccgggacca
    10081 tatccagccc gccagtacgt tgaagaacgg gcagggtgaa atccattgat gtggcaccgc
    10141 ataagcccag tgcagtagag cggctgcggc gaatcagccc agggatcaac ataatagcaa
    10201 tcagttcacg ggccagatca ttaaaaaacg ccgcgctccc gattaccgga ccaaaagatt
    10261 cggtcaataa aataccggaa agagaatacc agccgaaacc ggaggccatt gccagcgcgg
    10321 tattgatggg gagatcaaga ataaaggcgt taattaaacc accaattaat gaactgacaa
    10381 ccaccaccac ggcgacaatc attccccggc gattaaggac aatctgcttt aaggtcatgc
    10441 cattattgcg caactgaata ccaacgagga aaagtagcaa aattaacgtg tattcactgg
    10501 cttcggtcgc gtgttgtaag aaagccagtc cactcagacc aatggcaaaa ccaatcacta
    10561 ctacgccgca cagttttagc gactccagcg ccatcgcaat acgcgacggg agtttttctt
    10621 gctgatggtg gttgcgccac ggcaggcctc gctccagcca catcagggcg gcaatattac
    10681 acagtaaaat aacggtaata ctgacggcag aataatgcag aatcgccaac aggttactgg
    10741 cgaggttatc gagaaacgcc agactgatac ccataaaaaa gagaataagg taaaccatcc
    10801 agcttaatag ctgattaata acttttaacg cagcttgttg gcgaagcgga atgaggtaac
    10861 ctacaatcag gggaaccaga atgattaaca gcccagaaaa catgaaaacc cagtccttgc
    10921 aaagatgaag tcgaaatgcg cgatgacaca ctactgaaag cggaaggacg agtaaagttg
    10981 caattaaaag gaaatgttat gcataaggag cagtagagta ttcgttttca tttaaagata
    11041 ttcttgcgct ttaattacaa actgcaccga tgttggtggc gtcaaaatcg ccgaggcgtt
    11101 ccctgaaggc cggggcagcc cacatggatg tgggc
//
LOCUS       DAZPLI010000130        10853 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZPLI010000130 DAZPLI010000000
VERSION     DAZPLI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.131"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            80..421
                     /locus_tag="ACHUU3_003248"
     CDS             80..421
                     /locus_tag="ACHUU3_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR2083246.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(412..1338)
                     /locus_tag="ACHUU3_003249"
     CDS             complement(412..1338)
                     /locus_tag="ACHUU3_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR2083247.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGWEGAPLYQI"
     gene            1428..2426
                     /locus_tag="ACHUU3_003250"
     CDS             1428..2426
                     /locus_tag="ACHUU3_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR2083248.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2423..2641)
                     /locus_tag="ACHUU3_003251"
     CDS             complement(2423..2641)
                     /locus_tag="ACHUU3_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR2083249.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2643..4658)
                     /gene="ligA"
                     /locus_tag="ACHUU3_003252"
     CDS             complement(2643..4658)
                     /gene="ligA"
                     /locus_tag="ACHUU3_003252"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR2083250.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4729..5715)
                     /gene="zipA"
                     /locus_tag="ACHUU3_003253"
     CDS             complement(4729..5715)
                     /gene="zipA"
                     /locus_tag="ACHUU3_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR2083251.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            5945..6706
                     /gene="cysZ"
                     /locus_tag="ACHUU3_003254"
     CDS             5945..6706
                     /gene="cysZ"
                     /locus_tag="ACHUU3_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR2083252.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLNVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6891..7862
                     /gene="cysK"
                     /locus_tag="ACHUU3_003255"
     CDS             6891..7862
                     /gene="cysK"
                     /locus_tag="ACHUU3_003255"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR2083253.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8246..8503
                     /gene="ptsH"
                     /locus_tag="ACHUU3_003256"
     CDS             8246..8503
                     /gene="ptsH"
                     /locus_tag="ACHUU3_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR2083254.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8548..10275
                     /gene="ptsI"
                     /locus_tag="ACHUU3_003257"
     CDS             8548..10275
                     /gene="ptsI"
                     /locus_tag="ACHUU3_003257"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR2083255.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10316..10825
                     /gene="crr"
                     /locus_tag="ACHUU3_003258"
     CDS             10316..10825
                     /gene="crr"
                     /locus_tag="ACHUU3_003258"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR2083256.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         2642 a         2797 c         2691 g         2723 t
ORIGIN      
        1 gatttgtaag attgattttt cttctcaagt tccttgttct tctgccgata ttacctcgta
       61 cagtgacgag gactgaacaa tggccactat ctcttcaact tcaattcctt ccatacaaac
      121 acagagtagt aacagggcat cacaaggtag cgatgttgct tcaaaaatcg cgcggataag
      181 tcagcaaatc atcaaactca ctcaacaaat taaagagatt gtagacacta gcggcagtgc
      241 tgaagacaaa caaaaacagg ccgaattgat tcaacagcaa atcaccctac tggaaacaca
      301 actggcgcag ttgcagagac agcaggcaga aaaggcgcag gagaaagagc agcgtttgtc
      361 tctgaatgta tcccttctaa atcctgttga aaatacgact catatcgata tctatatctg
      421 atacaacgga gccccttccc aaccagccat tagcgcattg ccctggtctc gtaccacgtc
      481 ccataacgct tcagcggcgg tggaaagtga acggttcttc ctgcgcacca gcatcaactg
      541 gcgttcaaca accggcgtaa tgcgctttac caccagtggg ctaccttcag gcaatggtag
      601 cgccagtgcg ggaagaatac tgatgccgat gcctgcggct accatcggga acagcgtcgc
      661 cggatgtcca atctcctgca caatattcgc ctgaatacca ttgcgcgcca gcgctgcgtc
      721 aatcagcggt cggctgcctg acgcgtaatc ctgcaacacc aatttcgctc cctgtaatgc
      781 ctgccagggt acataatctt ccacggccaa agcgctatcg cgatggcaaa gcagaaagaa
      841 aggctcggaa agaatcgctt cacattgcag atcgcccact gggcctggat cgatgacgat
      901 gcaaaaatcg acatctccct ggcgaatact ttccatcacc cactgctgcg gacgatcgtg
      961 caggacaaac tgaatatctg gatagcggcg atggctttca gcaatgcatt gcggaataag
     1021 atgcgcggaa atggtctggc tggcagcgac ccgaactttt ccgctcagtt gttgccccat
     1081 acgcccggta tcgcgcaacg tgctgttaag ttcatccagc agtcgctcaa gacgcaaagc
     1141 caactgctgc cctgcatctg taagcaccac ttcacgcgtg gttctgtcca gcaggcgaac
     1201 accagtatga ttttccagct ccttcacact gtgacttact gccgactggc tcaagccgat
     1261 acgctctcct gcacgactaa aacttttctc ctgcgctact gtgacgaaaa cctttaattg
     1321 ttttaaagaa taattcatct gttttcttca tgaatggatg caataaatca attttatttc
     1381 tcaaactgga agaagcacaa tagaaccatc gatcatctgg agtctttatg aaactttttc
     1441 gtatcctcga tcctttcacc ttaaccctga tcacggtggt gttgctggcc tctttctttc
     1501 cggccagagg cgatttcgtc cccttctttg aaaatctgac caccgcagct attgccctgc
     1561 tgttctttat gcacggcgcg aagttgtcgc gtgaggcgat tattgctggc ggtggtcact
     1621 ggcgactgca tttgtgggta atgtgcagca ccttcgtgct gtttccgatt ctgggtgtac
     1681 tgtttgcctg gtggaaaccg gtaaatgtcg acccgatgct ctactccggt tttctctact
     1741 tgtgcattct cccggctacc gtgcagtctg caatcgcctt cacgtcaatg gcgggcggta
     1801 acgtcgcggc ggcggtttgt tctgcgtcag catccagcct gctggggatt ttcctttcac
     1861 cattgctagt tggtctggtg atgaatgttc acggtgcagg gggcagcctt gagcaggtcg
     1921 gtaaaattat gctgcaactg ctgctgccgt ttgtgttggg gcatctttcc cggccgtgga
     1981 ttggtgactg ggtgtcgcgc aataaaaaat ggattgcgaa aactgaccag acgtccattc
     2041 tgttggtggt ttatacagcg ttcagcgaag ccgtcgttaa tggtatctgg cataaagttg
     2101 gctggggatc attgctgttt atcgtggtgg tcagctgcgt tcttctggct atcgtgattg
     2161 tagttaacgt ctttatggca cgccgactgg gcttcaataa ggcagatgaa attactatcg
     2221 tcttttgtgg ttcgaaaaag agtctggcaa atggcatccc gatggcaaac attctgttcc
     2281 ccacatcggt gatcggtatg atggtgctgc ccctgatgat tttccatcag atccaattga
     2341 tggtctgtgc ggtgctggcg cgtcgataca aacgccagac cgaacagtta caggcgcagc
     2401 aggaaagcag cgccgataaa gcttaaagcg gacgcttcag gggctggacc agttgcgtca
     2461 gcccctcggt tttaatcagc agcgtgattt gcattagctc accgagcttc cctgccggga
     2521 actcatcttt gcgggcaaac cacagcagat actcctccgg caggtcgatt aagcgacgcc
     2581 ctttgtattt gccaaacggc attatcgtat tggctatttc aatcagctgc tctttttcca
     2641 tctcagctac ccagcaaacg cagcatttcc gcttcgtcga tgacttcaat gcccagttcc
     2701 tgcgccttcg ccagtttaga tcctgcagct tcacccgcta tcaccagatc ggttttcttc
     2761 gacacgctgc ccgcgacttt cgcgcccagt tcgaccagtc gagctttagc gtcatcacgc
     2821 gacatctggc ttaagctgcc cgtaagcacc acggttttac cagcaaacgg gctgtcaatc
     2881 tcttccgcgt tgataacgat cggcgcaggc cagtgaacac cttccgccaa cagctcgctg
     2941 atgacattgc ggttgctttc ttcggcaaag aagttgtgaa cgtgggatgc aacgacaatg
     3001 ccaacatcag gcaccttttg cagctcttca atcgaagcgg cttccagcac ttccagcgtg
     3061 ccgaaatatg ccgccagacc tgctgcggtg gcctcgccga cttcacggat gccaagtgca
     3121 tagaggaagc gagcaaaggt ggtttctttc gctttttcca gcgcgttaac cacgttttgt
     3181 gccgattttg gccccatacg ctccagtccg gtcagtttgc ctgcggtgag tttgaacaga
     3241 tctgccggag tgtggacata ttctttttca accagctgat cgatgatttt gtcgcccatt
     3301 ccgtcaacat ccatcgcacg gcgggaaaca aagtgtttca gcgactcttt acgctgcgca
     3361 ccgcaaatca ggccaccggt acagcgggca accgcttcac cttccacacg ctcaacgtca
     3421 gaaccacata ccggacaatg cgtcgggaat acaacctcac gggtatcttc cgggcgttca
     3481 gaaagcacga cgttaaccac ctgcgggatc acgtcgccag cgcggcgaat caccacttta
     3541 tcgccaatgc gtaaaccaag acgttcgatt tcatccgcat tgtgtaaggt tgcgttactc
     3601 accagcacgc cagcaacatg gacaggttcc agacgcgcaa caggcgtaat cgcgccagta
     3661 cgcccaacct gaaactcgac gtcacgcaca aaggtcatct gctcctgcgc cgggaattta
     3721 aacgctaccg cccagcgcgg ggcacgcgcg acaaagccaa gctgctcctg ctgttccagt
     3781 gagttgacct taatcaccac gccgtcgata tcaaagccca gcgtcgggcg gtcttcttcc
     3841 actttgtgat agaacgccag cacttcttcc gccgattcac aaagcgttac ccgatcgctg
     3901 accggcaacc cccacttttt aaattgcagt aaacggccaa gatgagtatc cggcagctcg
     3961 ccaccttcca gaacaccaac gccatagcag aaaaaagtga gcggtcgctt cgctgtaata
     4021 cgcggatcaa gctgacgcag tgaaccagct gccgcattac gtgggttagc aaaaactttc
     4081 ccgcccgtgc gtcgcgcatc ttcgttaatc ttttcgaacc ccgcctgcgg caggaacact
     4141 tcaccacgca cttccagacg cgccgggata ttctctccgt gcagcttcag cggaatggcg
     4201 cgaatagtac gcacattaga cgtgatatct tccccggtgg tgccatcgcc acgggtcgcg
     4261 gcactgacta aaacgccatt ttcatacaga atactgacgg caaggccatc cagcttcagc
     4321 tcacagcacc aggtgacttt ctcgttgttt ttcagacggt cctgcacacg tttgttgaaa
     4381 gcaagaaagc tttcttcatc aaaaacgtta tccagtgaca gcattggtac ttcatggcgt
     4441 atctggctga aagccgccag cggcgcagca cctacacgtt gggtaggcga atcaggcgta
     4501 atcagttctg gatgtttggt ttccagctcg cgcagttcgc gcatcagcct gtcgtattca
     4561 gcgtcgggaa tttccggcgc atccatcaca tgataaagat attcatgatg gcgaagcgtc
     4621 gttcgcagtt ctgtcagttg ttgttcgatt gattccatat cgcaccatca atgctaaaaa
     4681 cccccgacaa gcgggggttc gaagaggagt taatttgcct taagtgtatc aggcgttggc
     4741 gtctttgact tcgcggatga tgtcctggta ctcgcgcaat ttctgcggag tcatcatacg
     4801 gcgctgatcg tcaagcacga caccgcccac ttcatcggca atatgctgcg cagattgcag
     4861 catcagcttg aagttctgca gctcgtcacc gtaagacggt acctgcataa agatagtgac
     4921 acccggagta gtgaaatcct tcatttcagg atcaaaggtt cccggtttca ccatattcgc
     4981 caggctgaat aacgccgggc cgctgccatc cgggctaaga tgacgatggt aaatattcat
     5041 atcgccaaaa atgaagcccg cttgttgaat gctgttaaga agcagttcac cgtttagctc
     5101 gctaccgtga tgcgccgcga cgttcatgat aatcaccgct tctttgcgct tcggtttatc
     5161 cataactgga gccggttccg ctacaggctc aggctgtggt gccgctacgg gttctgcagg
     5221 ctggaaagcc tgttgtgccg gttgcggtgc tgaatgcaca ggctgcggcg ctggcgcgac
     5281 ctgtggcgaa actggctgct gcaacggctg ttcaggctgc ggctgatagg caggctgctg
     5341 catcggctgc gctggacgcg gagcatgttg cggcggtacc tgcgcttcag gcggctgctg
     5401 gatcggttga cgcggctgcg cagacgcata aggcggttgg tactggtgtt gcggcgacgg
     5461 acgagcagcc tcatgctcct gagcgttagc cggggcatga ttcacgcggt gaacacgaac
     5521 ctcaccaacg ccctcatcat cttcgacatc ctcgtcataa gaatcgtcgt cacgttttga
     5581 cttcattcgt tttaatggcc gatcgcggaa catagaagat cgttctttac ggctggtcca
     5641 gaaaccatgt accagtaaag cgattatggc gatcgcgcca acaatgatta atatcagacg
     5701 caaatcctgc atcattatat tctctgttgt tctaacacct tgccaccacg gcaaacattt
     5761 actcactaag agtatttgcc gattacctca agtgcaagtg cactattaac tttcacagca
     5821 caaagataga tgaaatcgtg ctttttgctg ttttttcgaa catatcctaa ctgtccattg
     5881 cgcaattacc cggtaaaata cgcagaattt tcctgggatt ggtcaaaagg agctcatcct
     5941 gattatggtt tcatcattca catctgcccc acgcagcggt ttttactatt ttgcgcaagg
     6001 ctggaagctc gtctcgcaac ctgggattcg gcgtttcgtt attttaccgc tgctggtcaa
     6061 tattttgttg atggggggcg cattctggtg gctctttaca cagctcaatg tctggatccc
     6121 gactctcatg agttacgttc cggactggct acaatggctg agttatctgt tgtggcctct
     6181 ggctgtcatc tctgtgctgt tagtgtttgg ctatttcttc tccacgattg ctaactggat
     6241 tgccgctccg tttaacggtt tattggctga acaactggaa gcacgattga ctggcgctac
     6301 accgccagat accgggattt tcggtatcat gaaagatgtg ccgcgaatca tgaaacgcga
     6361 atggcaaaaa tttgcctggt atctgccgcg cgcaattgta ttactaattc tttacttcat
     6421 ccctggtatt gggcaaaccg tcgcgccggt actgtggttc ctgtttagcg cctggatgtt
     6481 agccatccag tattgcgatt accccttcga taaccacaaa gtgccgttta aagagatgcg
     6541 caccgccctg cgcacacgca aaatcaccaa tatgcagttt ggtgctttaa ccagcctgtt
     6601 tacgatgatc ccgctgctta atctgttcat catgcccgtt gccgtttgtg gcgcgacggc
     6661 gatgtgggtc gattgctatc gcgataaaca cgcgatgtgg cggtaacaat ctaccggtta
     6721 ttttgtaaac cgtttgtgtg aaacaggggt ggcttatgcc gccccttatt ccatcttgca
     6781 tgtcattatt tcccttctgt atatagatat gctaaatcct tacttccgca tattctctga
     6841 gcgggtatgc tacctgttgt atcccaattt catacagtta aggacaggcc atgagtaaga
     6901 tttttgaaga taactcgctg actatcggtc acacgccgct ggttcgcctg aatcgcatcg
     6961 gtaacggacg cattctggcg aaggtggaat ctcgtaaccc cagcttcagc gttaagtgcc
     7021 gtatcggtgc caacatgatt tgggatgccg aaaagcgcgg cgtgctgaaa ccaggcgttg
     7081 aactggttga accgaccagc ggtaataccg ggattgcact ggcctatgta gctgccgctc
     7141 gcggttacaa actcaccctg accatgccag aaaccatgag tattgaacgc cgcaagctgc
     7201 tgaaagcgtt aggtgcaaac ctggtgctga cggaaggtgc taaaggcatg aaaggcgcaa
     7261 tccaaaaagc agaagaaatt gtcgccagca atccagagaa atacctgctg ctgcaacaat
     7321 tcagcaatcc ggcaaaccct gaaattcacg aaaaaaccac cggtccggag atctgggaag
     7381 ataccgacgg tcaggttgat gtatttattg ctggcgttgg gactggcggt acgctgactg
     7441 gcgtcagccg ctacattaaa ggcaccaaag gcaagaccga tcttatctct gtcgccgttg
     7501 agccaaccga ttctccagtt atcgcccagg cgctggcagg tgaagagatt aaacctggcc
     7561 cgcataaaat tcagggtatt ggcgctggtt ttatcccggc taacctcgat ctcaagctgg
     7621 tcgataaagt cattggcatc accaatgaag aagccatttc taccgcccgt cgtctaatgg
     7681 aagaagaagg tattcttgca ggtatctctt ctggagcagc tgttgccgcg gcgttgaaac
     7741 tacaagaaga tgaaagcttt accaataaga atattgtggt tattctacca tcatcgggtg
     7801 agcgttattt aagcaccgca ttgtttgccg atctcttcac tgagaaagaa ttgcaacagt
     7861 aatgccagct tgttaaaaat gcgtaaaaaa gcaccttttt aggtgctttt ttgtggcctg
     7921 cttcaaactt tcgcccctcc tggcattgat tcagcctgtc ggaactggta tttaaccaga
     7981 ctaattattt tgatgcgcga aattaatcgt tacaggaaaa gccaaagctg aatcgatttt
     8041 atgatttggt tcaattcttc ctttaacggc ataatgttta atgacgtacg aaacgtcagc
     8101 ggtcaacacc cgccagcaat ggactgtatt gcgctcttcg tgcgtcgcgt ctgttaaaaa
     8161 ctggcgctaa caatacaggc taaagtcgaa ccgccaggct agactttagt tccacaacac
     8221 taaacctata agttggggaa atacaatgtt ccagcaagaa gttaccatta ccgctccgaa
     8281 cggtctgcac acccgccctg ctgcccagtt tgtaaaagaa gctaagggct tcacttctga
     8341 aattactgtg acttccaacg gcaaaagcgc cagcgcgaaa agcctgttta aactgcagac
     8401 tctgggcctg actcaaggta ccgttgtgac tatctccgca gaaggcgaag acgagcagaa
     8461 agcggttgaa catctggtta aactgatggc ggaactcgag taatttcccg ggttctttta
     8521 aaaatcagtc acaagtaagg tagggttatg atttcaggca ttttagcatc cccgggtatc
     8581 gctttcggta aagctctgct tctgaaagaa gacgaaattg tcattgaccg gaaaaaaatt
     8641 tctgccgacc aggttgatca ggaagttgaa cgttttctga gcggtcgtgc caaggcatca
     8701 gcccagctgg aaacgatcaa aacgaaagct ggtgaaacgt tcggtgaaga aaaagaagcc
     8761 atctttgaag ggcatattat gctgctcgaa gatgaggagc tggagcagga aatcatagcc
     8821 ctgattaaag ataaacacat gacagctgac gcagctgctc atgaagttat cgaaggtcag
     8881 gcttctgccc tggaagagct ggatgatgaa tacctgaaag aacgtgcggc tgacgtacgt
     8941 gatatcggta agcgcctgct gcgcaacatc ctgggcctga agattatcga cctgagcgcc
     9001 attcaggatg aagtcattct ggttgccgct gacctgacgc cgtccgaaac cgcacagctg
     9061 aacctgaaga aggtgctggg tttcatcacc gacgcgggtg gccgtacttc ccacacctct
     9121 atcatggcgc gttctctgga actgcctgct atcgtgggta ccggtagcgt cacctctcag
     9181 gtgaaaaatg acgactatct gattctggat gccgtaaata atcaggttta cgtcaatcca
     9241 accaacgaag ttattgataa aatgcgcgct gttcaggagc aagtggcttc tgaaaaagca
     9301 gagcttgcta aactgaaaga tctgccagct attacgctgg acggtcacca ggtagaagta
     9361 tgcgctaaca ttggtacggt tcgtgacgtt gaaggtgcag agcgtaacgg cgctgaaggc
     9421 gttggtctgt atcgtactga gttcctgttc atggaccgcg acgcgctgcc cactgaagaa
     9481 gaacagtttg ctgcttacaa agcagtggct gaagcgtgtg gctcgcaggc ggttatcgtt
     9541 cgtaccatgg acatcggcgg cgacaaagag ctgccataca tgaacttccc gaaagaagag
     9601 aacccgttcc tcggctggcg cgctatccgt atcgcgatgg atcgtaaaga gatcctgcgc
     9661 gatcagctcc gcgctatcct gcgtgcctcg gctttcggta aattgcgcat tatgttcccg
     9721 atgatcatct ctgttgaaga agtgcgtgca ctgcgcaaag agatcgaaat ctacaaacag
     9781 gaactgcgcg acgaaggtaa agcgtttgac gagtcaattg aaatcggcgt aatggtggaa
     9841 acaccggctg ccgcaacaat tgcacgtcat ttagccaaag aagttgattt ctttagtatc
     9901 ggcaccaatg atttaacgca gtacactctg gcagttgacc gtggtaatga tatgatttca
     9961 cacctttacc agccaatgtc accgtccgtg ctgaacttga tcaagcaagt tattgatgct
    10021 tctcatgctg aaggcaaatg gactggcatg tgtggtgagc ttgctggcga tgaacgtgct
    10081 acacttctgt tgctggggat gggtctggac gaattctcta tgagcgccat ttctatcccg
    10141 cgcattaaga agattatccg taacacgaac ttcgaagatg cgaaggtgtt agcagagcag
    10201 gctcttgctc aaccgacaac ggacgagtta atgacgctgg ttaacaagtt cattgaagaa
    10261 aaaacaatct gctaatccac gagatgcggc ccaatttact gcttaggaga agatcatggg
    10321 tttgttcgat aaactgaaat ctctggtttc cgacgacaag aaggataccg gaactattga
    10381 gatcattgct ccgctctctg gcgagatcgt caatatcgaa gacgtgccgg atgtcgtttt
    10441 tgcggaaaaa atcgttggtg atggtattgc tatcaaacca acgggtaaca aaatggtcgc
    10501 gcctgtagac ggtaccattg gtaaaatctt tgaaaccaat cacgcgttct ctatcgaatc
    10561 tgatagcggc gttgaactgt tcgtccactt cggtatcgac accgttgaac tgaaaggcga
    10621 aggcttcaag cgtattgctg aagaaggtca gcgcgtgaaa gttggcgata ctgtcattga
    10681 atttgatctg ccgctgctgg aagagaaagc caagtctacc ctgactccgg ttgttatctc
    10741 caacatggac gaaatcaaag aactgatcaa actgtccggt agcgtaaccg tgggtgaaac
    10801 cccggttatc cgcatcaaga agtaattctt gccgcagtga aaaatggcgc cca
//
LOCUS       DAZPLI010000131        10848 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZPLI010000131 DAZPLI010000000
VERSION     DAZPLI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10848
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.132"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..2180)
                     /gene="traC"
                     /locus_tag="ACHUU3_003259"
     CDS             complement(<1..2180)
                     /gene="traC"
                     /locus_tag="ACHUU3_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFR2083257.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGVPFCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAATQFPLPEGMNLPLTLRHYR
                     VFFSYCSPSKKKSRADILEMENLVKIIRASLQGASIATQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNRKVGEFIEKGYRTARRHTGA"
     gene            complement(2306..2668)
                     /locus_tag="ACHUU3_003260"
     CDS             complement(2306..2668)
                     /locus_tag="ACHUU3_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083258.1"
                     /translation="MKSIYLESVLAFIFVGVMAMLICGLFYNDYLEQQPATPEQLTEI
                     TQDTPCAAEAFKEAIKSDTSDYQPEPLSLGKAKELASACRERNEMAEVKRVRENERNK
                     IREKQLQALNDAHSVKER"
     gene            complement(2699..2910)
                     /locus_tag="ACHUU3_003261"
                     /pseudo
     CDS             complement(2699..2910)
                     /locus_tag="ACHUU3_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195235.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2990..3466)
                     /locus_tag="ACHUU3_003262"
     CDS             complement(2990..3466)
                     /locus_tag="ACHUU3_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083259.1"
                     /translation="MHKSENEKIDFILDEHERIFRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRRVLSRQELLWLMSLTEDCPDM
                     HQELLNCLLSGKKLTGLDKREIRSLWWEKMDAMQESATRQREQDTIRKFTEGNKSE"
     gene            complement(3459..3680)
                     /gene="traR"
                     /locus_tag="ACHUU3_003263"
     CDS             complement(3459..3680)
                     /gene="traR"
                     /locus_tag="ACHUU3_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFR2083260.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(3815..4330)
                     /gene="traV"
                     /locus_tag="ACHUU3_003264"
     CDS             complement(3815..4330)
                     /gene="traV"
                     /locus_tag="ACHUU3_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFR2083261.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTTSVQAVAATTPSRSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVVPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(4327..4578)
                     /gene="trbG"
                     /locus_tag="ACHUU3_003265"
     CDS             complement(4327..4578)
                     /gene="trbG"
                     /locus_tag="ACHUU3_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HFR2083262.1"
                     /translation="MNNLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRNTVFENPDIVKTCTVRDLKNDFMNCDEKGEGQ"
     gene            complement(4571..4891)
                     /locus_tag="ACHUU3_003266"
     CDS             complement(4571..4891)
                     /locus_tag="ACHUU3_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFR2083263.1"
                     /translation="MNMRNINVITARVVPDKNVSDDFMHAVLSNCTTKIVLPAPKVFS
                     SESLPHNFNMAAVGVMKKGMSMSLFSSPGHIVVAGQSGGGKSVPAKELIDRIIQKYKE
                     PFDE"
     gene            complement(4878..5468)
                     /gene="traP"
                     /locus_tag="ACHUU3_003267"
     CDS             complement(4878..5468)
                     /gene="traP"
                     /locus_tag="ACHUU3_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFR2083264.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTAFWATLALMYVSLAAITGKYPVRPGKMKCVRVGTADEHLK
                     EVYTEDTSLSEKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(5458..6885)
                     /gene="traB"
                     /locus_tag="ACHUU3_003268"
     CDS             complement(5458..6885)
                     /gene="traB"
                     /locus_tag="ACHUU3_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFR2083265.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGALYLSDVDMAGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALEQSQSTAEEGNPESPVPNPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(6885..7613)
                     /gene="traK"
                     /locus_tag="ACHUU3_003269"
     CDS             complement(6885..7613)
                     /gene="traK"
                     /locus_tag="ACHUU3_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFR2083266.1"
                     /translation="MRKNNTAIIFGSLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(7600..8166)
                     /gene="traE"
                     /locus_tag="ACHUU3_003270"
     CDS             complement(7600..8166)
                     /gene="traE"
                     /locus_tag="ACHUU3_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFR2083267.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(8188..8499)
                     /gene="traL"
                     /locus_tag="ACHUU3_003271"
     CDS             complement(8188..8499)
                     /gene="traL"
                     /locus_tag="ACHUU3_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFR2083268.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFALPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSFWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(8514..8873)
                     /gene="traA"
                     /locus_tag="ACHUU3_003272"
     CDS             complement(8514..8873)
                     /gene="traA"
                     /locus_tag="ACHUU3_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFR2083269.1"
                     /translation="MNTVLSVQGASAPVKKKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVG
                     FAIISVFIAVGMSVVGL"
     gene            complement(8906..9301)
                     /gene="traY"
                     /locus_tag="ACHUU3_003273"
     CDS             complement(8906..9301)
                     /gene="traY"
                     /locus_tag="ACHUU3_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-bindin protein
                     TraY"
                     /protein_id="HFR2083270.1"
                     /translation="MKRFGTRSATGKMVKLKLPVDVEGLLIEASNRSGRSRSFEAVIR
                     LKDHLHRYPKFNRAGNIYGKSLVKYLTMRLDDETNQLLIAAKNRSGWCKTDEAADRVI
                     DHLIKFPDFYNSEIFREADKEEDITFNTL"
     gene            complement(9400..10089)
                     /gene="traJ"
                     /locus_tag="ACHUU3_003274"
     CDS             complement(9400..10089)
                     /gene="traJ"
                     /locus_tag="ACHUU3_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator
                     TraJ"
                     /protein_id="HFR2083271.1"
                     /translation="MYPMDRIQQKHARQIDLLENLTAVIQDYPNPACIRDETGKFIFC
                     NTLFHESFLTQDQSAEKWLLSQRDFCELISVTEMEAYRNEHTHLNLVEDVFIQNRFWT
                     ISIQSFLNGHRNIILWQFYDAAHVRHKDSYNQKTIVSDDIRNIIRRMSDDSSVSSYVN
                     DVFYLYSTGISHNAIARILNISISTSKKHASLICDYFSVSNKDELIILLYNKKFIHYL
                     YEKAMCIINTR"
     gene            complement(10276..10659)
                     /gene="traM"
                     /locus_tag="ACHUU3_003275"
     CDS             complement(10276..10659)
                     /gene="traM"
                     /locus_tag="ACHUU3_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFR2083272.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVHEAQMERKESAFSQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDDE"
BASE COUNT         2738 a         2855 c         2383 g         2872 t
ORIGIN      
        1 gcaccggtat gacggcgggc ggtacggtaa cctttctcaa taaactcacc gaccttgcga
       61 tttttgaagt ccagcagacg ccagccttca tcaataacgt tcagtttctt gaggttacgc
      121 ggcgtgcggt acatcctgtt ctcgatgtag ataatcaggg agaacatcac cgcaaccagc
      181 agtgacggac ggtcttccag tccgcccagc tccagcacca ccattttcgc atcatcccgc
      241 agggacggct catcggagtt aaaatactgg ccgtaagtgc cgttggcagt gtactggtca
      301 agcagcacaa tcatttcgtc cagacggctg cggatggtcg gtgacccggc atactgctcg
      361 ctgtcactgg cgtttttcag gaaatccacc acgtcatcaa tacgtgccct gttctctttg
      421 gccagccagg aggccctgac cgcctgcagc agcagacctt catgcacttc atccaggtta
      481 ccgttggggc tggccatcac tgacaactgg tcacggacac gctccgctga ctggtcaata
      541 tcggtgatgt tcgcaaacgg gttaaaacgc agggtttcac cgtccagata tacgccaccc
      601 atgttctcac acagggactt gtagccatcc cccatgtcga acaccacggc aaagcccccg
      661 gagtccagca cgctgcggat aagtggctgt atcagcccgg ttttaccggc cccggaggtg
      721 ccacagaccg ccatgttgta gttggtgtta ttcatccccc ggaagaaaat atcgataaac
      781 gccagctggt tacggtaggt gggtgccagc agacctgccg gtgtcagggg gttatccgcc
      841 actaacggca tcaggttggc aacattaaag ctctctgcgc gctgaaccac tccggcctct
      901 ttcagctgtt taaacaaccc tttcccggcc ataaagggca gacaggtcag gaaattgcgc
      961 atgtggttaa agcgcggtga aatcagctca aaaccgtttt tacggaagct gttcaggatg
     1021 tcctgctcca cttccagcgc cgtttcattg ttgtccttgc agaatgctgt gatgttgagg
     1081 aagtaggaca cgacagaaga ctgaccggag cccagacgct gacgcagttc cccccactcc
     1141 ttcgcctctt tctccacgga cggaaaccat ttggcatagg aggtcttcga ctttttctcc
     1201 aggtccatgt acttcaggtt ggcttcgctg tgggttttca cctggtcttc caccaccaga
     1261 gtcagcgtca ggatgaacgg acaggagatg gacagttccg ggttcagcag gttgctgtag
     1321 ttgtcggcca tattccacag gaaggcgatt tccgggttac gggccagatg gaaattcagg
     1381 atgcgggccg tgctgttcct gccgttctca cgcaggccca gcgtcaggta atcagcccgg
     1441 actttcaggt caaaactgtc ctccacacac tgataattca gatcagaata cgggtccagc
     1501 tgacgtcttt tcgggtacag ggagtccggg ttatgattaa tcatctcccc gacaatatcg
     1561 ataaaggcct gtgcatccac cgtctgtgtg gcgatactgg ccccctgtaa cgacgcccgg
     1621 atgattttca ccaggttttc catttccaga atgtcggccc ggcttttttt ctttgaggga
     1681 gaacagtacg agaagaatac ccggtaatgc cgcagggtca ggggcagatt catcccctcc
     1741 ggcagcggaa actgtgtcgc tgccgcgttc atataatagg cccgggtaat ggcgttaaac
     1801 cgttcagcct gttcacctga ccaggagaac tcacgcagcc cgtattcaat tctgtcacca
     1861 accagctggc tggacatcag atggatgcag aacggcaccc cgcgcggcag tttggtgcgc
     1921 agcatgtggt cgagcgcctc cacaatggac tcattcgccc cattgatggg aatggcttcc
     1981 agcataaagc ccatcgtggt gtcattcatg aacaggccgg actcctggtt gtaatcacgg
     2041 tacggcagca gacggctgaa ctgcgggaag ctcatttcgc ccagaacttc attggccttt
     2101 gcggattcgt caggcagttt cagtgctgtg acgagggagt taaccgcctg agtgacggcc
     2161 tcaagtgggt tattactcat atgagtattc cttagtgcgt ttaaaaaagg aaaatatcag
     2221 tctgtggccg tggatatatc acgcagtgcc attcctgaaa ccacagcaca aataagagat
     2281 ggaggctttt aacaggaaac caggattaac gttcctttac tgaatgagca tcatttaatg
     2341 cctgaagttg tttttcacgt atcttatttc gctcattttc tcttacccgt ttcacctctg
     2401 ccatttcgtt tctttctcta cacgctgatg caagctcttt tgccttacca agacttaaag
     2461 gttcaggctg ataatcagat gtgtcagact taatagcttc tttaaatgct tctgctgcac
     2521 agggggtatc ctgtgtaatc tctgtcagtt gttctggtgt tgcaggttgt tgctcaaggt
     2581 aatcattgta aaaaagacca catatcaaca tagccataac gccaacaaat ataaaagcca
     2641 gaacagattc taaatatatc gacttcattt cattttcctt tgaagcagta agcactcatt
     2701 accccccatt cagtgtatcc atcacgttgt tcaaaggttt atttgtttta acctcaatac
     2761 attcccctgt atttccaaag cccctgcctc cgtgtgcttc gattgtccga agaacctgca
     2821 cgcatacatc cgtatttggc atggggtaac attccagctt ccgccaccgt taatattttt
     2881 tatcagcaca agaaaaataa aggtatgcat tctttattac ctccgcttat atattaatca
     2941 ctgaatatcc tttcagttaa cttatgaggt cataaaacac tgcagattct cattcggatt
     3001 tattcccttc agtaaacttc cgaatagtat cctgttcacg ctgacgggtg gcagactcct
     3061 gcattgcatc catcttttcc caccagagac tcctgatttc cctcttgtcc agaccggtca
     3121 gttttttacc ggacagcagg cagttaagca gttcctggtg catatccgga cagtcttcag
     3181 tcagactcat aagccagagc aattcctgac gactgagtac cctgcgtgat tcctccggta
     3241 cgctgtaata attaaaaata cggctgaata ccccacaaca gcaggcaacg gacaggcaca
     3301 gggatttcag atacaggcca gaaacacaga atgtggcaac accacaaaga atagctataa
     3361 ctaaaaagcc cttacccagt gccatacaca aagaaccctg ttttctgaat atccgctcat
     3421 gctcatccag aataaaatct attttttcat tttctgattt atgcatagtg tttcctctgc
     3481 ctttcctgat acgcctgaca ttcaacgcac aacgtcacac cgggaaatat tttccgccgg
     3541 gcttccggaa taggatttcc gcatgcttca cagagataaa caggaatacc atgaacattt
     3601 attttctggc gtatccggtt tattcctgtc atggtcagtt gttctgtcac tgaatatgct
     3661 tcatcggctt catcactcac ggaatattcc tcccgacatg ttctgacacg ctaccgccca
     3721 tcccttttca cgcacagggc actcgtttta caggcagatt ttgaatacag aaagcccgcc
     3781 ggcattgccg gctgatatac aacttcaggg ccagttaatt aatacgtggt tttccccacg
     3841 cagacggttt aataacaaaa aacacactgg atggctgatg ataaacatcc tgattatcaa
     3901 tataaggcgc tatccataat gcagccgtct gctcacccgt tcttagcgga cggggaggtg
     3961 ttaccggcac aactgaactg accggaacaa ccgttttcac ttcccgggca gcggtaaaca
     4021 gcgggcgtgg tgccagcagt ttctgctccg gatgtgctgt caccgcaggc ctgctgcggg
     4081 acggcgttgt cgccgcaacg gcctgcaccg acgtggtccg gaagttccct tcagccaggc
     4141 gcggcagtga tgccgcaacc ggctttgctt ctgaggactg ctccagtttt ttggccttct
     4201 cattagcctg ctccatcgtc atacaggtat cggatgtggt ggcgttacac tcaaattccg
     4261 tactggttcc ggcacagccg gaaagtaata aggtccccag cagaggaata aataaagaaa
     4321 tctgtttcat tgtccctcac ctttctcatc gcagttcata aaatcattct tcaaatccct
     4381 gaccgtacag gtttttacaa tgtcaggatt ttcaaaaacc gtattccgtg atgatttata
     4441 aacatatgcc acaatgcgtt ttccacccgc atcaggttct ggtgcactga cttcacataa
     4501 cggaggtgtt attccggact catacagaac cggataattt atttttttga cgctgccatc
     4561 agaaaccaga ttattcatcg aagggttcct tatatttctg aataattctg tcgattagtt
     4621 ctttcgccgg aacagatttt ccgccaccgg attgtccggc aaccacaata tgccccggag
     4681 aagaaaataa gctcatcgac attcccttct tcatcacacc gacagcagcc atattaaaat
     4741 tgtgcggcaa tgattcagaa ctgaacacct tcggagcagg aagaactatt tttgtggtgc
     4801 agttactgag aacagcatgc ataaaatcat ctgatacgtt tttatccggc acaacgcggg
     4861 ctgtaataac attaatatta cgcatgttca tttttatctc cgttgcttct gtttgttcgg
     4921 tcatcaggga aataaacatg gcatttcctg attttttccg acagagaagt atcttccgta
     4981 taaacttcct tcagatgttc atctgcggtt cccacccgga cacatttcat ttttccgggt
     5041 cgcaccggat atttcccggt gatcgctgcc agcgagacat acatcagtgc cagtgttgcc
     5101 cagaatgcgg tgaccagttg catcagtgac cggtcagtct ccctgacata atctgcagca
     5161 tcagccgcgc ggtaatggca gatatcctgt atttctgatc tgcctgaaga atcagaggaa
     5221 gaaaaccagc attcactgac cgggaactgt ccggcaggtg ctgtttgtct tacgaagtta
     5281 atctccttaa ccagtaattt ccccggcgtg gtgttgtcct tccataaaac aaacagagcc
     5341 atcagggcca ttgtggccag tggcagaatg acggtgtact tcagacacca gaacagtcct
     5401 cgcagaaccc gggcgacaac ataccgggcg gcatggcacg cctgacggga cgacatatta
     5461 tttgccatcg ttgcctcctc cctggctgcc ggacataaac tgctgcggat tcagattgcc
     5521 cagctgcttc agcatctggt cggtgttaaa accgttcagg tggctttctg ccgagggagg
     5581 attcggcaca gggctttccg ggttaccctc ttccgctgta ctctgtgact gctccagcgc
     5641 catttcctcc acggttttca gctggaagcc gtcctggaac accactgtca cttcgttgcc
     5701 cgcaccaatc ggtatcaccg ggtgatactg ttcggcacgt ttgatgtagt agtcactgag
     5761 cgtctgtgcg gctttcgatg cgccgccacc gatacccatt ttaaggacat cgccggcccc
     5821 gtaagcagca gtggcaccca gcccgacagc cggctgggag gcgcgctcca ttccctgacc
     5881 gataccgtca acaaatcccg cgccccatgc ccagccgagg attttgccgt tacgcatcac
     5941 cacttcgccc ttgataccgt ttttaccccg gaagctgaca tgccccttaa tcggcatatc
     6001 aatagttttg ccgtccttca ggcagctaat attgcgggtg cgtacaatgg cacgctcact
     6061 ggacacatcc ccccaggctt ccagacccac aaaacatccc gttgcgtcat acgtcttgct
     6121 gttcggcatt tccaccagac cggtgatacg cagctgcatc ggcaccgtgg attcattacc
     6181 ggtgactgag gcattggcat ccgccccttc aatcagcatg gctttcgcaa aacttcctga
     6241 cggaatgtac ggcagcgatg gtccctgttt tccctcatta cgggtaaaca ccttacgctg
     6301 tatccggtta ggcaccggca cagactggta cgtcacctgt ggtggtggcg tgacaccatt
     6361 cccgggataa aacgccgtcg gcggtggaac agcaacgctg ccctgcggcg ggaaggatac
     6421 gggggtgttt cctggctgtg gttcgccttc cggcccgggc ggtgaggctg gcatctgcgg
     6481 tacaggctca ccggtcgcgg tgacgggatt tgcacccagg gcttttacct gctctgccag
     6541 ggcagcatta tcctgtccca gcttttcaat acgacgctgg tcatcaccgc gctgtttgtt
     6601 caggacatcc agttcacgac ggatttcctc atactgcttc tgcatctgcg ctgccgtcac
     6661 ctgcatctct gtggtggcat gctgacgcac cttgtcatca aaggtcgtat ccaccacacc
     6721 ggtcatatcc ggcacaggct cctgttcagc caccgcctca ccgttaccgg ccatgtccac
     6781 atcagacaga tacagtgcac caccaatcgc ggaggctgca ccgacaacca caatccccag
     6841 ccacaggtac tgcttgcgtt tcacaatggt attgatactg gccatcagtt gccctccccg
     6901 tcacggatga catacacatc catgcgcgca cctgccagta actggctggc aggctggctg
     6961 aacatcacgg cacgggttcc cggctgccag aaatcacttt cccggatatt cagggcagac
     7021 agcgttttat tttccacggc aaagcggacc atcttcaggt gattccccgt ccatacggca
     7081 tctgccactg aagacagccc cgccggggcc tgcagggttt cctttgtcac aggaacctga
     7141 taccagcctg caggtaattt tccgccacgg acagcctggc tgatggtcac aagcagggat
     7201 tcgtaaggcg tggacgtttc ccacgcaccg gcttcttctc cggttccgcg caggtcactg
     7261 accagctgaa tggtacgccc cgcgccttca cggggaacgg cctgaatgga aagattcaga
     7321 ccacgttctg tttccagaat gaacgtaaag ggctttttgt tgacggtggc aaccaccaca
     7381 ccgccggagg cggtctgctc attattggtc agggcaccat ccagactgtt cacggcgata
     7441 atccggtcac cgggaacggt aaacagattc gggctggtat tgctgatggc aatactggcc
     7501 tgaccaccgt tcaccattgg caccacggtg ggggccagcg taccgtttgc ggccatcacg
     7561 ctgcaggaaa aaaacaggct gccgaatatt attgccgtat tattttttct catcgtctgt
     7621 ttccccgaaa ttatccagcc aggtcacccc gttttcccgc ttcagaataa ggatgtaatg
     7681 tttgatatca gtgaaaggtt ttgaatcacc aatccaggtt ttaagcacac cacgaatttc
     7741 cacacggcca tactgaggcc atacacggac actggtctgg aaaaaggctg agttcacgtt
     7801 atcgttttta atacgcttcg cttcttcagc cagaataact ttcatctggt tctgtgcgcc
     7861 cgggcggata tattgcagaa gcgcctgatg tgaggcatcg acggtttctg atgaaacatt
     7921 cagacggagg gcaataaatg acagcgccat ctgctgtaaa taagaggcgt cggcactgtt
     7981 ctgtgacacg gcaaagggaa cattaaatcc cattggggtc acggcagtgc gttgctcatt
     8041 ctgaagacgg tagttattca ccccctgaat gacgttaaca gagaggctga gaacaataag
     8101 cactgacata aatataaagg cgatggccat tacacgactg gtacttaaac gggcaccgtg
     8161 ttccatataa caatcctggt atcagttcta tttaatccac tgccggaaac acgaatcggg
     8221 aacattatga aaaataccgc gcaacagggc tgttggcata taccagtaaa tcaggtcacg
     8281 taaccagaaa ctgccccgcc cttttttcag ttttttaatc ccgaaataaa ccagaaccgc
     8341 tgcaccaata ccgaacagat atttcgatgt tgtgataccc cagccaatac agattgcggc
     8401 ggggatcagt tcatccagtg gcagggcaaa ccagcggctc tggttggtca gtgtttccgg
     8461 gaaacgatat ttcttaagtt tattctcgtc tcccgacatc gttttatttc ctgtcagagg
     8521 ccaacgacgc tcatacccac agcaataaat acagagatga tggcaaagcc gaccaggaac
     8581 ttgacgtttt tggtcatcat gtacatgacc gcaccgacca gcacctcagc cagaacaacc
     8641 catttaacga tactggagtc cttaccgaag gtcgccttaa ccgtatcgtt acctttagcc
     8701 atcaggtcgc ctttaccagc agccatagct aactcaggga acaacatcat cagaacagca
     8761 accgggatca ctgcacgaac caggcgaagc atattcagac gagtgaattt agaaaaaaac
     8821 gacttctttt tgacgggcgc agaagcaccc tgaacactta aaacagtatt cataagttac
     8881 ctcaatttcg gataaatgaa taaaactaga gtgtattaaa tgttatatct tcctctttat
     8941 ctgcctccct gaatatctcc gagttataaa aatcaggaaa cttgatcaaa tgatcaataa
     9001 ctctgtctgc agcctcatct gttttacacc atccactacg attttttgct gcaataagta
     9061 gctggttagt ttcatcatcc agacgcattg tcagatactt aaccagcgac ttaccataga
     9121 tattccctgc cctgttaaac tttggatagc ggtgaagatg atctttaagt cttattactg
     9181 cctcaaacga tcgacttctt ccgcttctgt tacttgcctc aattaataga ccttccacat
     9241 ctacaggtaa ttttagtttt accatcttac ctgttgcaga acgtgtacca aatcttttca
     9301 ataacacctc ccgctgttta tcttctggtt actacttatg tttgcagaat aatcagaaaa
     9361 ggtaaataga cggaaagtct atattttgtt aacactttat taacgcgtat ttatgataca
     9421 catagccttc tcgtacaaat aatgaataaa ctttttattg tagagtaaga tgattaactc
     9481 atctttatta gaaacagaga agtagtcgca tatcagagac gcgtgtttct ttgatgtgga
     9541 gatggatatg tttaatattc ttgctatagc attatgactg attccggtgc tatataagta
     9601 aaagacatca tttacatatg atgatacaga agaatcatca ctcattcttc tgattatatt
     9661 tctgatatca tcactgacaa tcgttttttg attataactg tctttatgac gaacatgagc
     9721 agcatcataa aattgccaca gaatgatatt tctgtgtcca ttaagaaatg actgaataga
     9781 tattgtccag aatctgttct gaataaaaac atcctctaca agattaagat gcgtatgctc
     9841 attcctgtat gcttccatct ctgtgacaga gatcaattca caaaaatctc tctgcgacag
     9901 aagccatttt tcagcacttt gatcttgtgt aagaaatgac tcatgaaaca gcgtattgca
     9961 aaaaataaat tttccagttt cgtccctgat acaggctgga tttggataat cctgaataac
    10021 tgccgtcaga ttttccagca gatctatttg acgagcatgt ttttgttgaa tacgatccat
    10081 cggatacata ggaacctcct cacaaaggat tctatggaca gtcgatgcag ggaggagaga
    10141 actccctgca tcggcgattt tcaaattcaa attttaacgt ggcattaacc acgtaattaa
    10201 tatactaatt gtctttcgta ctgtcacctt tttaaatcat aaaaactctg atatttgaac
    10261 gaagtcaaat ttcttttatt catcatcatt ttttggaaaa aatcgttcca tctcagatga
    10321 taccttctcc ctgatatctt caaccatatt ggcatattca aactttggat ttccggagac
    10381 atgaggactg agagactcaa tacccaaaat tttcgctact gatgattgtg tttttacaac
    10441 gcattcaaga agcaatttat taaactcagt ctgactaaat gcagactctt tacgctccat
    10501 ctgagcctca tgtacacgaa gccccagttc aagaagcatt gaagctgttg ctgaaaaact
    10561 gacatctttt tcccttgccc cttcctgtcg acgcttctca ataatcgcat ttattttttc
    10621 ataggcatca ttgctgatat acaggttcac cttagccatt agagattacc ttttttgaac
    10681 ataaaataaa gttaatttcg aaacactaaa attcataatg caaacaggga cgcaccgcta
    10741 gcagcgcccc tagcggtatc ctataaaaaa acacaccgcg ccgctagcag cacccctaat
    10801 ataaaataat gttttttata aaaatagtca gtaccacccc tacaaagc
//
LOCUS       DAZPLI010000132        10835 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZPLI010000132 DAZPLI010000000
VERSION     DAZPLI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10835
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.133"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..279)
                     /locus_tag="ACHUU3_003276"
     CDS             complement(<1..279)
                     /locus_tag="ACHUU3_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR2083273.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSR"
     gene            complement(446..1186)
                     /locus_tag="ACHUU3_003277"
     CDS             complement(446..1186)
                     /locus_tag="ACHUU3_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR2083274.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1279..1914)
                     /gene="argO"
                     /locus_tag="ACHUU3_003278"
     CDS             complement(1279..1914)
                     /gene="argO"
                     /locus_tag="ACHUU3_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR2083275.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2053..2913)
                     /gene="mscS"
                     /locus_tag="ACHUU3_003279"
     CDS             complement(2053..2913)
                     /gene="mscS"
                     /locus_tag="ACHUU3_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR2083276.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3104..4183)
                     /gene="fbaA"
                     /locus_tag="ACHUU3_003280"
     CDS             complement(3104..4183)
                     /gene="fbaA"
                     /locus_tag="ACHUU3_003280"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR2083277.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4398..5561)
                     /gene="pgk"
                     /locus_tag="ACHUU3_003281"
     CDS             complement(4398..5561)
                     /gene="pgk"
                     /locus_tag="ACHUU3_003281"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR2083278.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5611..6630)
                     /gene="epd"
                     /locus_tag="ACHUU3_003282"
     CDS             complement(5611..6630)
                     /gene="epd"
                     /locus_tag="ACHUU3_003282"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR2083279.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7002..7433
                     /locus_tag="ACHUU3_003283"
     CDS             7002..7433
                     /locus_tag="ACHUU3_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFR2083280.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7456..8034
                     /locus_tag="ACHUU3_003284"
     CDS             7456..8034
                     /locus_tag="ACHUU3_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR2083281.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8035..8742
                     /locus_tag="ACHUU3_003285"
     CDS             8035..8742
                     /locus_tag="ACHUU3_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFR2083282.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8730..9407
                     /locus_tag="ACHUU3_003286"
     CDS             8730..9407
                     /locus_tag="ACHUU3_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR2083283.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9401..10078
                     /locus_tag="ACHUU3_003287"
     CDS             9401..10078
                     /locus_tag="ACHUU3_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR2083284.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10050..10763)
                     /locus_tag="ACHUU3_003288"
     CDS             complement(10050..10763)
                     /locus_tag="ACHUU3_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR2083285.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10760..>10835)
                     /locus_tag="ACHUU3_003289"
     CDS             complement(10760..>10835)
                     /locus_tag="ACHUU3_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR2083286.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2580 a         2880 c         2699 g         2676 t
ORIGIN      
        1 tcgtgaacga cgccctgagt tatggatttc attatcaatc gcagaaagtc gttgataaac
       61 cgggaacagt ttttgataca gttcctgacc ggctttattg aggctgatat tattatcttt
      121 gcgggtaaat agcgtatagc caatttgttc ttccagcgcg gcaatgcttt taccaaatgg
      181 cgaagccgtc atatggattt tttctgccgc ccgggcaata ttattggttt gcgctaataa
      241 aatgaaattg cgcatctttt tcgaaataaa aatgtccatc ccccctcccc gcatccttgt
      301 ccgcttaacc gtttcagtca gcctatcctt gatgaaaccg cgagcaaaga taggtgatta
      361 cgtcatggtt ttacagaaaa ttacagaaaa aggaggcaat atcgggtaaa ggcattagcc
      421 cgacgaatac gtcgggctac aagtattatt gtgctgcagg tgttttagcg ggttgttgat
      481 ccacaggttc taactggaag accacatcga cctgatcatc aaactgaata gcggcctgct
      541 cgtaagtttc ctgggcagac accggcgcgg catcggcttt catcatccgc accattgggc
      601 tgggctgata gttggaaaca tggtagcgca cgctatatac cggccccagt ttacgatgaa
      661 agccgttcgc cagttcctgc gcctgatgaa tcgcgttatc aatcgctgcc ttacgcgctt
      721 tgtctttata ggcatccggc tgcgccacgc ccagcgacac agaacgaatt tcgttaagac
      781 ccgccttcag cgcgccatcc agcaaggaat tcagtttgtc taactgacgg agcgtgactt
      841 ccaccgttct cacagcgcgg tagcctttca ggatactttt accatcctga taatcataat
      901 ctggctgggt gcgtaagttc gctgagctga tatctttttt cgcgatctga ttgagttcaa
      961 ggaaggaaat gtattgtgcg acgcgctcat ctgcctgttt cttggctgta gcggcatcct
     1021 tcgcggccac gttaacttca atcgcaagag tggcaatgtc tggcaccgca tccacacttg
     1081 ccgtaccgga ggtgacaata tgcggtccat ccggcaattc gttagcctgc gctgccatcc
     1141 cgctaatacc cattaatgcc gccagggcga taactttgaa cttcacgtta gtctctccgt
     1201 gttaattgac ttgcccaaaa ttcaggacct ctggaggcaa gcttagcgcc tctgttttat
     1261 ttttccatca gatagcgcct aactgaacaa ggcttgtgca tgagcaatac cgtctctcgc
     1321 cagctgcaag gctataaacc acatgacaca tcccactacc agattgataa tgcgctgtga
     1381 ttttgccgtg cgcaggcgcg gtgccagcca ggctgcgaga atagccagac caaagaacca
     1441 caggaaagag gcgctaattg tcccgagcgc aaaccaacgt tttggttcga cgtcaagctg
     1501 cccgccaagg ctgcccagta caacaaaagt atccaggtaa acatgcggat tcagccaggt
     1561 cactgccagc atggtggcga taattttcca tctgccttgc ttcaggactt cggcactagc
     1621 taactcaata ttactactca tcgctgtttt aaaagcgcca aaaccatacc acagcaagaa
     1681 ggctacgccg ccccaggtga ccagcgccag caaccacggc gactgcatca ataacgcgct
     1741 gccaccaaaa atcccggcgc aaatcaggac caaatcgctg atagcacaaa gtaaggcaat
     1801 cataatgtgg tactgacgac gaatgccctg attcatcaca aaagcatttt gtggaccgag
     1861 cggcagaatc atagctgccc caagagcaat accttgaaag taataagaaa acacgttgga
     1921 tattccgaat taagtatctg atgcagagac tatatcgcgg gattgttatt agcggaaatt
     1981 gataatatta attggcaatc agaataacta ataagagaga ggccagagag tattatctgg
     2041 cctcagcgtt gattacgcag ctttgtcttc tttcacccgc ttaaagttca catccatttg
     2101 cgggtacggg aagctgatac cggcggcatc aaattcacgt ttaatacgct ccagcacatc
     2161 ccagtacacg ttttgcagat cgccgctgtt gctccagacg cggaccacga aattaatcga
     2221 cgatgcacca agttcgttca gacgcacagt catttcgcga tctttcaaaa tgcgatcttc
     2281 agactggata atattggtca ggatctgctt aacctgatcg atatcggaat catacgccac
     2341 gccaataata aattcgttac ggcgaactgg ctcgcgagag aagttaataa tatttccggc
     2401 aataatttta ccgttcggaa taacgataat tttaccgtct gcagtacgca tggtggtgga
     2461 gaaaatctgc acactcagca cagtaccggc tacgccgccg agatcaacat attctccggc
     2521 acggaacggg cggaacatga caagtaacac gccagcggcc aggttagaaa gtgacccctg
     2581 caaagccaga ccaacagcta agcctgcggc accgagtaca gcaatgactg acgctgtttg
     2641 tacacccacg cgtcccaggg cagcgattag cgtaaaggcg ataataccgt aacggactaa
     2701 tgcagaaaga aaatcagcaa cagtggcatc gattttacgg gagatcatca ggcgattcac
     2761 cgcgttggaa atcatccgcg cgataatcaa accaacgatg atgatcgcga gtgccgccac
     2821 gatgtttact gcataactta gcagcagcgc ctggttagct accagccagc ttcccgcgcc
     2881 gtttatgcta tcgacaacat tcaaatcttc cattcaatat tccttttcaa ctgactcaaa
     2941 atggagaaat acactccgcc gccttatgac gggcagtctg acaagggtaa acaataaagc
     3001 ataattttgc caaatagatc acagatttct catctgaatc accttgcttt tggcgaaaaa
     3061 aagacccgca gagcgggcct tgagataagc agaaaggaat atcttacaga acgtcgatcg
     3121 cgttcagttc ctggaatgct ttttccagac gagcgatcat cgaagtctga ccggcacgca
     3181 gccatacgcg cggatcgtag tatttcttgt tcggctgatc ttcgcctttc gggttaccca
     3241 actgcccctg cagataagct tcgttcgctt tgtagtagtt cagaacgcct tcccaggttg
     3301 cccattgggt atcggtatcg atgttcattt ttactacgcc gtagcttacg gagtctttga
     3361 tttcctgagc agtagaaccg gaaccaccgt ggaatacgaa gttcaggctg ttgtgcggca
     3421 ggttgtgttt cttggaaaca tattcctgag aatcacgcag gatggtcgga gtcagaacca
     3481 cgttacccgg cttgtaaaca ccgtgtacgt taccgaagga cgcagcgatg gtgaaacgcg
     3541 ggctgatttt gctcagttcg gtgtatgcgt aatcaacgtc ttccggctgg gtgtacagtg
     3601 cagaagcgtc catgtggctg ttgtccacgc cgtcttcttc accaccggtg caacccagtt
     3661 cgatttccag agtcatgccg attttggaca tgcgctccag gtatttagag cagatttcga
     3721 tgttctcttg cagagattct tcagacaggt cgatcatgtg agaagagaac agcggcttac
     3781 cggtagctgc gaagtgtttt tcacccgcgt ccagcagacc gtcgatccac ggcagcagtt
     3841 tcttcgcgca gtggtcagtg tgcaggataa ccggaacacc ataatgttca gccatctggt
     3901 gaacgtgatg cgcaccagag atcgcgccca ggatagcagc accctgcgga acgtcagatt
     3961 tcacgccttt accagcgata aaggaagcac caccgttgga gaactgaacg ataaccggcg
     4021 ctttaacttt agcagcggtt tccagtacgg cgttgatgga gtcagtaccg acgcagttta
     4081 ctgctggcag tgcgaagttg ttttcttttg ctacctggaa aactttctgt acgtcatcac
     4141 cagtgattac gccaggtttt acgaaatcaa aaatcttaga catgtctctt gtcctgtatc
     4201 gtcgggcctt atactcatca tacttcaagt tgcatgtgct gcgtctgcgt tcgctcaccc
     4261 cagtcactta cttatgtaag ctcctgggga ttcactctct tgtcgccttc ctgcaactcg
     4321 aattatttag agtatgaaaa aatgcgtgct ctaaaagcgc gctgaaacaa gggcaggttt
     4381 ccctgccctg tgatttttta cttcttagcg cgctcttcga gcatcgctac tgcaggcagt
     4441 actttacctt ccacgaattc gaggaatgcg ccgccgccag tggagatgta ggagattttg
     4501 tcagcaatgc cgaacaggtc gattgctgcc agagtgtcgc cgccgccagc gatggagaac
     4561 gcttcgctgt ctgcgatagc gttagccacg atttcagtac ctttgcggaa gttcgggaat
     4621 tcgaacacgc caaccggacc gttccacaga atggttttcg cattcttcag gatttcagcc
     4681 agttcctgag cggaagcatc accgatatcc aggatctgct cgtcagcttt cacatcgtta
     4741 acagatttca gggtagccga tgcagtttca gagaactcgg ttgctacgcg aacatcagac
     4801 ggaaccggga tgttgcaggt ggtcagcaga cgtttagctt cgtcaaccag gtcagcttcg
     4861 tacagggatt tacccacatc gtggccttgt gccgcgataa aggtgttagc gataccacca
     4921 ccaacaatca gctggtcagc gattttagac agggagtcca gaacggtcag tttggtagat
     4981 actttagaac caccaacgat agccaccatc gggcgagcag gttctttcag tgctttaccc
     5041 agcgcgtcca gttcagctgc caacagcggg cctgcgcacg caacatcagc gaatttaccg
     5101 ataccgtgag tagaagcctg cgcgcggtga gcagtaccga atgcgtccat tacgaacacg
     5161 tcacacagtg cagcgtattt tttggacagg gtttcgtcgt ctttcttctc gcctttgttg
     5221 aagcgaacgt tttccagaac aaccagttca ccttcagcaa cgtcaacgcc gtcgaggtaa
     5281 tctttaacca gacgaaccgg gttagacagt ttgtctttca ggtagttaac aaccggcagc
     5341 agagagaatt cttcgttgta ctcgccttcg gtaggacgac ccaggtggga agttaccatc
     5401 actttcgcgc cttgtttcag ggccagttcg atggtcggca gagaagcacg gatacgcgcg
     5461 tcgctggtta ctttcccgtc ttttactggt acgttcagat ccgcacggat aaatacacgt
     5521 ttcccagcaa gatccagatc ggtcatctta attacagaca tggtgaatcc tctcgttgat
     5581 tctaaaagtt ttgcagacgc tgcttgcgtc ttacctgaaa gcaacagtag ccatagctaa
     5641 cgtcgtgtcg agcattcggt tagcaaagcc ccattcgtta tcgcaccaga ccaacgtttt
     5701 gatcaggtgt gcgccactga cccgggtttg ggtgccatcg acaatggcac tgtgcggatc
     5761 gtggttaaaa tctacagaga ccaacggcaa ttccgtatag tcaactatac catgaaatgc
     5821 accttgtgct gctttttgca gcaacaggtt gacttcattg gcttttacag gtttcttcac
     5881 cgtcacgctt aaatcgattg ccgtcacatt tatggttggc acacgtaccg caatcgcttc
     5941 aaagcgatcg ttaaattgcg gaaaaaatcg tgtgataccg gcggccagtt tagtatcgac
     6001 cggaatgatc gactggctgg ctgcccgggt gcgacgcagg tcaggatggt atgcatcaat
     6061 aacctgttga tcgtgcatgg cggagtgaat tgtggtcaca gtgccggact caataccgta
     6121 cgcatcatct aacaatttga tgacgggaat tatgcaattc gtggtacagg aagcgttaga
     6181 aacgatgcgg tgttccgcac gaagttgatc ctgattgacg ccgtaaacaa cggtcgcgtc
     6241 gagatcgtta ctgccaggat gtgaaaagag cacttttttg gccccggcgg caatatgcgc
     6301 ttcgccatgc tcgcgggagc catatacgcc ggtgcagtcg aggactacat caacgccaag
     6361 ttcacgccag gggagcgatt gcagtgaacg ttcatgcaat acgcggatgg cgtcatcacc
     6421 aacaaaaagt tgatcgcgtt cctgtcgtac ttcccatgca aaacggccat ggctggtgtc
     6481 atatttcaac aaatgcgcca tgcccgcagc atccgccagt tcgttgattg ccaccacggt
     6541 aatttccgcc cggcgtccgg attcatacaa agcacgaacc acattacgcc cgatgcgacc
     6601 gaagccattt atcgctacgc gtacggtcat agatctcctg caaggttttc cctgagcaaa
     6661 tttgccagac agagtaatcc agcaaatcgt ccggggaaac cttacctgtc gcaaactgcg
     6721 actgattggt taattgtcga acatttaatc gactgaaacg cttcagctag gataagcgaa
     6781 acgtggaata aaaggaatga ttgtccagtc aaagaaaaca attatctgac ttacgtcacg
     6841 ttttcagccc cgcccctgtg gaatttattc cactttgcag aatcacgaat aagcgtagca
     6901 aaatcttttc gttgtgccgt cacaaaccct gacttatatc agaatatgac tgatgcagcg
     6961 tcgatattat ccgcaatacg tccgagtttc ggagtactcc aatgatgaca ttgccctacc
     7021 catattcttc ccccgcacgc ttttatgcac tgcgcctcct ccccgggcag gaagtttttt
     7081 cccagctaca cgcttttgca cagcagcatc aacttcatgc cgcctggatt gcaggctgta
     7141 ccggtagcct gactgacgtc gccttgcgct atgccgggca agaaggcacc acgctcctta
     7201 acggtacatt cgaagtgatc tcactaaacg gcacgctgga acagagtggt gagcatcttc
     7261 atctctgcgt ttccgatcca cacggcacga tgcttggcgg tcatatgatg cccggctgta
     7321 ccgtgcggac cacgcttgaa ctggtgatcg gtagccttga ggaactggcg tttagccgcc
     7381 aaccttgcgc gctttctggt tacgacgagt tgcatatttc ccccgttaaa taactacttt
     7441 caaagaggtt ttcttatggc gcgtagccac ttttccagtc aggcactggt gttgatcgtt
     7501 atatctatcg ccatcaatat gatcggcggg caactggcca gcatggtcaa attacctata
     7561 tttctcgact ccatcggcac acttattagc gcggtactgc ttggtccggt gatcggaatg
     7621 ctgaccggct tactgaccaa tttactctgg ggattgctga ccgacccgat cgccgccgca
     7681 tttgcacccg tcgcgatggt catcggcctg gtcgccggtt ggctggcgcg agcgggttgg
     7741 tttcgcaccc tgccaaaagt cgttgtcagc ggcgtgatta ttacgcttgc ggtaacggtc
     7801 gtcgctgtac cgctgcgtac tgcgctgttc ggcggtgtca ccggtagcgg agccgatttg
     7861 ttcgttgcct ggatgcactc gatggggcaa aacctggtgg agtcggtggc gattacggtg
     7921 ataggcgcga acctggtcga taaaattctt acagcggtga ttgtctggct tttactgcgc
     7981 cagctgccga ttcgcaccac gcgccatttt ccggcaatgg ctgccgtgcg ctaaatgcat
     8041 ccatttacct cattaacact gtgggcattg gcggcctgca ccacgcttat cctgcccgcg
     8101 cagaccattt tgccgatcta tagcgcggcg accttttttt gtcttattgc gctaaaggcc
     8161 acgcggcggc gggcaaaata tgtcgcctgg ctgatgttct cgctgggagc cgggctgtgg
     8221 ctggtacacg gtggctggct aacagaatgg ctaagcggca cgccgcgttc gccggagcgt
     8281 tggacgcatg ctattacgct ctggttgcgg attctggcga ttgtttcaac ctcacagttg
     8341 tggatgcagt acgtccccgt gcaatgtttt atccgcgctc tgtttgcttc tcgcctgccg
     8401 ccaggcgtcg cttatctgtt tgccggaccg ttattggtcg ttgagcagtt aaaacggcaa
     8461 ttagcgatta ttcatgaagc gcagcgtgcc cgcggcgtgc cgctggatga gggctggtat
     8521 cagcgtttac gtgcgatgcc cgcgttaatt attctgctaa cacacaatgc gcttaacgat
     8581 ctggctgtcc gtggtgcggc gctggatatg cgggcatttc ggatccacaa ccgacgaaca
     8641 acgttgtggg caccagccga tagcacccta caacgcgtag cgcgttacac catgatcttg
     8701 ctgatgctaa cagaattcgg agcgtggata tggttacgtt agaacagttc cgttattgcc
     8761 cgacacactc aacgcatcct ccgttctgtt atgactttca ctatgtcaaa ccggggatgg
     8821 tcgccatctt tggtgacaac ggcagtggca aaagtacgct ggcacagctg atggcgggct
     8881 ggtatccgga ctttctgcca ggagagataa caggcacggg gacgctcctc ggtacgccca
     8941 ttgggcatct cccgcttaat gaacaatccg ccaccatcca gctcgtccag caatcgccct
     9001 atttgcagct ctccggctgt acctttagcg tggaagaaga agttgccttt gggccagaaa
     9061 atctgtgtct tgctgaaaca gagattatgg cgcggattga tgccgccctg actctgaccg
     9121 agtgccagcc gttgcgccat cgtcacccgg caacactctc cggtggagag acacaacgcg
     9181 tggtcatcgc ctgcgccatc gcgatgcagc caaagctctt aattcttgat gaagccttta
     9241 gtcgcctgac gccgcaggcc agcgaaatgc tgttgcagcg cttacaacac tgggcgcttg
     9301 aacgtggttc gctgattatt ctttttgaac gtcatcgcac acctttcctg aactactgcc
     9361 agcaagcgtg gcaattgcaa aacggagcgc tccagccact atgttaacgt taaatcagat
     9421 atcttatcgc tggcctagcg cagcaacgga ttgtctttgc gacatttctc tgcaactcaa
     9481 acaaggtgaa tggttggcgc tgaccggcga caatggcgcg ggaaaatcga cgctgttacg
     9541 agtaatggca ggtcttctta cgcccacagc cggtacggtt atgctgcaac aacaggcgat
     9601 gaaaaacctg aaaaaccgcc agcgcgcggc aaaggttggc gtactgtttc aggaagcgga
     9661 aaaccaactt tttcacagca ccgtcgctga cgagatcgca tttggtttga aactgcaaaa
     9721 atgcccggcg gatgaaatca ctcaacgtac ccatgctgcc ctgcaatgtt gtcagttagc
     9781 agacactgcc aactcgcacc cgttggatct gcatagtgcg cagcgacgga tggtcgccgt
     9841 cgcctgcctg gaagcactct ctccgccact attactgctg gacgagccga gtcgggattt
     9901 tgacgaaaat tggttgagcg tgtttgaaag ctggctggag aagtgccgcc agcgaggcac
     9961 gagcgtggta gcaatcagtc atgacgccgc atttacccgg cgtcatttct ctcgtgtggt
    10021 gcgactggaa gatggtgtaa tcaggaacgt taatccacca gacgatattc acccgtagcg
    10081 gtcatcatta aggtcaaatt tgcaggcaga ctctcttcca gcacccggcg aacattcggc
    10141 ccgtcggtac ggtcataaaa ggcttctgca tctgccagcg atacccctcc cgccagcttg
    10201 cgccccacca gacgctcccg caacgcagta gctggcgcgt tgataaagat cgagaaatca
    10261 caaaattgcg caagctggca ccacttctcg tcgtccagca acaaccagtt tccttcgacg
    10321 atgacgagtg gtgcggtaac gtgcaacgca tcttcaacag gatcatgctt ttgtcgatcg
    10381 tactgcggcc acgtacaatc cccttccacc acctggcgca gattttccgc cagtttcgca
    10441 acgtcaaatg tctctggtgc gcctttgaag gggcgcaatt gatgcgcgtc cagccagcta
    10501 ttgtaatggt gaaaaccatc catcgggagc gtctgaatag cgggcagctc cgggtcttgt
    10561 tgcacgagat attcccagaa ggtggttagg gtagatttac ccgtccccgg cggagcgcaa
    10621 agaaaaacca ccgtacgccg ctgcggattt accgtctgta aggccgccag catacgcagc
    10681 aacggtttat gcacgcgttc tatctcttca tcatggtact gcgcttgcac gttcagccca
    10741 ttcaccgtta attcaatttt cacggcgtta attcctttaa ggtggtcgtt actgccagcg
    10801 tcagcgccgt tttgaccatt ccgctaaaac gcgcc
//
LOCUS       DAZPLI010000133        10686 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZPLI010000133 DAZPLI010000000
VERSION     DAZPLI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.134"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..784)
                     /locus_tag="ACHUU3_003290"
     CDS             complement(<1..784)
                     /locus_tag="ACHUU3_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR2083287.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="ACHUU3_003291"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="ACHUU3_003291"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR2083288.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="ACHUU3_003292"
     CDS             1860..2747
                     /locus_tag="ACHUU3_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR2083289.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASVVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="ACHUU3_003293"
     CDS             2780..3790
                     /locus_tag="ACHUU3_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFR2083290.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDVVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="ACHUU3_003294"
     CDS             3860..5293
                     /locus_tag="ACHUU3_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2083291.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="ACHUU3_003295"
     CDS             5392..6741
                     /locus_tag="ACHUU3_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2083292.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="ACHUU3_003296"
     CDS             6731..7618
                     /locus_tag="ACHUU3_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR2083293.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="ACHUU3_003297"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="ACHUU3_003297"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR2083294.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="ACHUU3_003298"
     CDS             8352..9224
                     /locus_tag="ACHUU3_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR2083295.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="ACHUU3_003299"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="ACHUU3_003299"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR2083296.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="ACHUU3_003300"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="ACHUU3_003300"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR2083297.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2462 a         2530 c         2855 g         2839 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagc attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgtcgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgttgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccgag taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatt gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacagggg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tctcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggt
//
LOCUS       DAZPLI010000134        10685 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZPLI010000134 DAZPLI010000000
VERSION     DAZPLI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10685
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.135"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            319..894
                     /gene="ssuE"
                     /locus_tag="ACHUU3_003301"
     CDS             319..894
                     /gene="ssuE"
                     /locus_tag="ACHUU3_003301"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR2083298.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            887..1846
                     /gene="ssuA"
                     /locus_tag="ACHUU3_003302"
     CDS             887..1846
                     /gene="ssuA"
                     /locus_tag="ACHUU3_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR2083299.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            1843..2990
                     /gene="ssuD"
                     /locus_tag="ACHUU3_003303"
                     /pseudo
     CDS             1843..2990
                     /gene="ssuD"
                     /locus_tag="ACHUU3_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            3002..3793
                     /gene="ssuC"
                     /locus_tag="ACHUU3_003304"
     CDS             3002..3793
                     /gene="ssuC"
                     /locus_tag="ACHUU3_003304"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR2083300.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3790..4557
                     /gene="ssuB"
                     /locus_tag="ACHUU3_003305"
     CDS             3790..4557
                     /gene="ssuB"
                     /locus_tag="ACHUU3_003305"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR2083301.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4764..7376)
                     /gene="pepN"
                     /locus_tag="ACHUU3_003306"
     CDS             complement(4764..7376)
                     /gene="pepN"
                     /locus_tag="ACHUU3_003306"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR2083302.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7642..8844
                     /gene="pncB"
                     /locus_tag="ACHUU3_003307"
     CDS             7642..8844
                     /gene="pncB"
                     /locus_tag="ACHUU3_003307"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR2083303.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVKFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9014..10414
                     /gene="asnS"
                     /locus_tag="ACHUU3_003308"
     CDS             9014..10414
                     /gene="asnS"
                     /locus_tag="ACHUU3_003308"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR2083304.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2446 a         2728 c         2848 g         2663 t
ORIGIN      
        1 ctttaaatga cattagactt aataataaca ataattgacc gccatttaaa tcaagcatac
       61 taaatccaat aagtgacaaa tctttaattt atagatttca tgaatattta aatattaact
      121 gaacgagtaa ttcattgatt caacatctaa tgactatcca acaaataaat atgcgtgata
      181 tataaagccc tttctttagt ttattttcag aaaaagatac acaaaattta tatttggcat
      241 tttcttgtct ctccgacaga ctgaccgaat tgttatcaat gttaacaaaa aaagaacaat
      301 tggttataag gagagagtat gcgtgtcatc accctggcgg gtagtcctcg ctttccttct
      361 cgctccagct ccttgctgga atatgcgcgg gaaaaactaa atggcctgga tgtagaggtt
      421 tatcactgga atctgcaaaa cttcgccccg gaagatctgc tttacgctcg tttcgatagt
      481 ccggcactca agaccttcac cgaacagctg caacaggctg atggactgat agtcgctaca
      541 cctgtgtata aagccgccta ttccggcgcg ctgaaaaccc tgctcgacct gctgccagag
      601 cgcgctttgc aaggcaaagt ggtgctaccg ctggcgacgg gcggtacggt ggctcatctg
      661 ctggcggtcg attatgccct taagccagtt ttaagtgcga tgaaagccca ggagctcctg
      721 cacggcgtgt ttgccgatga ctcacaagtt attgattacc atcacaaacc ccagttcacg
      781 ccaaatctgc aaacccgtct tgataccgcg ctagaaactt tctggcaggc attgcaccgc
      841 cgcgatgttc aggttcctga ccttctgtct ctgcgaggta atgtccatgc gtaacatcat
      901 taaactggcg ctggcgggat tgcttagcgt ctctactttt gcggttgctg cagaatcctc
      961 gcctgaagcg ttacgtatag gctatcagaa aggcagtatt ggtatggtat tggcaaaaag
     1021 ccaccagtta ctggaaaaac gctatccgca atcaaaaatc tcctgggtgg agttccccgc
     1081 gggtccgcaa atgttggaag cgttaaacgt tggcagtatt gatctcggca gtaccgggga
     1141 tattccgcca atctttgccc aggctgccgg ggctgatttg atatatgtgg gcgtcgagcc
     1201 accgaagccc aaagccgaag tgattctggt ggcagaaaac agcccgatca aaaccgtagc
     1261 cgatcttaaa ggtcacaaag ttgcctttca gaaaggttcc agttcacaca accttttact
     1321 gcgtgcactg cgtcaggccg gacttaagtt tactgacatc cagcccactt atctgacgcc
     1381 agctgatgcc cgcgccgcgt tccagcaagg taacgttgac gcctgggcta tctgggatcc
     1441 ctactactcc gctgcgttat tacagggcgg cgtgcgggtg ctgaaagacg gcaccgatct
     1501 caatcaaact ggatcgtttt atctggcagc tcgcccctat gcagaaaaaa acggcgcttt
     1561 tattcagggc gtactggcaa cctttagtga ggccgatgcg ttaacccgca gccagcgcga
     1621 gcaaagcatc gctttactgg caaaaacgat gggcttaccg gcaccggtga ttgcctcgta
     1681 tctggatcat cgtcctccca ccaccatcaa accggtgagc gctgaagttg ccgccttaca
     1741 gcagcaaacg gcagatctgt tttatgaaaa ccgtctggtg tcgaaaaaag ttgatattcg
     1801 ccagcgcatc tggcagccca ctcacctgga aggaaaacaa ttatgagtct gaatatgttc
     1861 tggtttttac cgacccatgg tgacgggcat tatcttggaa cggaagaagg ttcacgcccg
     1921 gttgatcacg gctatctgca acaaattgcg caagcggcgg atcgtctggg ctataccggt
     1981 gtgctgatcc caacggggcg atcgtgtgaa gatgcgtggc tggtggcggc gtcgatgatc
     2041 ccggtgacgc agcggctgaa gtttcttgtc gccctgcgtc ccagcgtaac ctcacctacc
     2101 gttgccgctc gccaggccgc cacgcttgac cgtctctcaa atggtcgtgc gttgtttaat
     2161 ctggtcacag gcagcgatcc acaagagttg gcaggcgacg gcgtgttcct tgatcatagc
     2221 gagcgctacg aagcctcggc ggaatttacc caggtctggc ggggcgctta ttgctggggg
     2281 aaagcgtcga tttcaacggt aaacatattc atgtgcgggg agcaaaactg ctcttcccgc
     2341 cgattcaaca gccgtatcct ccgctttact ttggcggatc gtcagatgtc gcccaggagc
     2401 tggcggcaga acaagttgat ctctacctca cctggggcga accgccagag ctggtaaaag
     2461 agaagatcga acaagtgcgg gcgaaagctg ccgcgcatgg acgcaaaatt cgtttcggta
     2521 ttcgtctgca tgtgattgtt cgtgaaacta acgacgaagc gtggcaggcc gccgagcggt
     2581 taatctcgca tcttgatgat gaaactatcg ccaaagcaca ggccgcattc gcccggacgg
     2641 attccgtagg acaacagcga atggcggcgc tacataacgg caagcgcgac aatctggaga
     2701 tcagccccaa tttatgggcg ggcgttggct tagtgcgcgg cggtgccgga acggcgctgg
     2761 tgggcgatgg tcctacggtc gccgcgcgaa tcaacgaata cgccgcgctt ggcatcgaca
     2821 gctttgtgct ttcgggctac ccgcatctgg aagaagcgta tcgggttggc gagttgctgt
     2881 tcccacatct ggatgtcgcc attccggaaa ttccccagcc gcagccgctg aatccgcaag
     2941 gcgaagcggt ggcgaatgat tttatccccc gtaaagtcgc gcaaagctaa ggaacacgat
     3001 gatggcaacg ccagtgaaga agtggttatt gcgcgttgcc ccctggtttt taccggtggg
     3061 catcgtggcg gtgtggcaac tggcctcctc ggttggctgg ctgtcgacgc gtattttgcc
     3121 ttcaccggaa ggagtggtga cggcgttctg gacgctctcc gccagcggcg aattgtggca
     3181 gcatctggcg atcagttcct ggcgggcgct gattggcttt tcaattggcg gatcgctggg
     3241 cttgattctg ggactgatta gcgggctgtc acgctgggga gaacggctgc tggatacctc
     3301 aattcagatg ttgcgcaacg taccgcatct ggcgctgatc ccgctggtga ttttgtggtt
     3361 tggcattgat gaatccgcaa aaatctttct ggtggcgctc ggcacgctat tccccattta
     3421 tatcaacacc tggcatggca tccgtaatat cgatcgcggt ctggtggaaa tggcgcgtag
     3481 ctatggatta tccggcatac cgctgtttat ccatgtgatc ctgcctggtg ccctgccctc
     3541 aattatggtc ggcgtgcgtt ttgcgttggg cctgatgtgg ctgacgctga ttgttgccga
     3601 aaccatttct gccaattcag gcattggtta tctggcgatg aatgcgcggg agtttttgca
     3661 aacggacgtg gtggtagtcg ctattattct ttacgccctg ctcggcaaac ttgctgacgt
     3721 cagcgcgcag ctgttagagc gcctctggtt gcgctggaac ccggcttatc atttgaagga
     3781 ggccacggta tgaatactgc tcgtctgaac cagggcacac cattgttgct caatgcagta
     3841 agcaaacatt acgcggaaaa tatcgtcctg aaccaactgg atttacatat tccggcaggt
     3901 cagtttgtgg cggtggtggg ccgcagcggt ggtggcaaaa gtaccctgct gcgcctgctg
     3961 gcaggtctgg aaacgccaac cgcaggcgat gtgttagcgg gcaccacacc gctggctgag
     4021 attcaggaag acacgcgaat gatgtttcag gatgcgcgtc tgctgccgtg gaaatcggtg
     4081 attgataacg ttgggttagg ccttaaaggc cagtggcgcg atgccgcgcg tcgggcgttg
     4141 gctgcagtag gactggagaa tcgcgccggg gaatggcctg ccgcactttc tggcgggcag
     4201 aaacagcgtg tggcgctggc aagagcgttg attcatcgac cgggtttatt gttgcttgat
     4261 gaaccgctcg gggcactgga cgccttaacg cgactcgaga tgcaggattt gattgtgtca
     4321 ctttggcagc agcacggctt taccgtactg ttggtgacgc atgatgtgag cgaagcggta
     4381 gcaatggctg accgggtgtt gttaattgaa gagggaaaaa ttggtctgga tttgacagtg
     4441 gatatccccc gtccgcgtcg attagggtcg gtgcgattag cggagctgga agcggaagta
     4501 ttgcaacggg taatgcggcg tgggcattct gaacaactga ttcggcgtca tggttaatat
     4561 gccggatgtg gcgcacaaaa ccagcatttt caacggactt gtaggcctga taagacacgt
     4621 caagcatcgc atcaggcagt tgggcacggc tgccggatgc ggcgtgaacg ccttatccag
     4681 cctacaaaac cagcaatttc aacggagttg taggcctgat aagacgcgtc aagcgtcgca
     4741 tcaggcattt tggctcaaaa gtatcaagcc agtgctttgg tgatcttctc gtacagatcg
     4801 ccagagagat tttccagccc tttcaactgt tccagcgccg cgcgcatttt ctcctgacgt
     4861 ttggcatcat aacgtttcag gcgaatcagc ggttcaatca gacgtgaagc tacctgcggg
     4921 ttacggctgt tgaggtcggt aagcatttcc accaggaact ggtaaccgct gccatcttcg
     4981 gcatggaacg ctgccgggtt gctgcccgca aacgcaccaa tcaacgaacg aatacggttc
     5041 gggttgctca tggtaaatga gcgatgctgc aacagaccac gcaccgtctc cagcacattc
     5101 gccgccgggc tggtggcttg caggataaac catttatcca tcaccagacc gtcctgatgc
     5161 cacttatcgt cgtactcctg catcagcgcg tcacggcaag gcagctgtgc agcaaccgcc
     5221 gcagaaagcg ctgccagcgc atcggtcata ttgtttgctt catggtattg cttgctcacc
     5281 agcacgtcag ccagatgcgt ttcaccaaaa gcaaggaagc gcaggcaggc atttcgcaga
     5341 gtgcgttttg caatatcttc atgctcaaca cggtactcgc tctggtaatt cgcgttgtaa
     5401 atagccagca gctcatccgc cagttcagtc gccagagtac gagtgagtgc ttcgcgtact
     5461 tcggcaatag caatcgggtc gatgatatcg aacagttcag ccatttcatt gacagaaggc
     5521 agcgtcagga tttctgccgc cagcgccgga tcaatcttct catcgagcag caccgcgcgg
     5581 aaagcgtcag ccacatgtac cggcagagac agcggctgcc cttgctgatg acgggcgacg
     5641 ttcagcttga tgtaggttgc cagcaaactt tgcgccgcat cccaacggga gaaatcatta
     5701 cgcgcatgac gcatcaggaa ggtcagttgc tgatcactcc acttatattc cagtttcact
     5761 ggcgcagaga attcgcacag cagcgcaggc accggctgga agtagacatt atcaaaaaca
     5821 aaggtctgtt ccgcctgggt gacgttcagc acggaattca ccggatgacc gcctttctgc
     5881 aacgggatca ctttgccttc gttgtcatac aattcgatgg caaacggaat atgcagcggc
     5941 tgtttttctg cctgatccgg cgtggctggc gtgcgctggc tgatggtcag ggtgtactgc
     6001 tcagtttccg gattgtagtc gtctttgacg gtcacaatcg gcgtaccgga ctggctgtac
     6061 caacggcgga aatgggagag atcaacattc gacgcatctt ccatcgcctg cacaaagtcg
     6121 tcacaggtcg ctgcgctacc atcatgacgc tcgaaataga gttgcatccc tttctggaag
     6181 ttttcttcgc ccagcagggt gtggatcata cggatcactt ccgcgccctt ctcgtaaacg
     6241 gtcagggtgt agaagttgtt catctcaatg accatgtccg ggcggattgg gtgcgccatc
     6301 gggctggcgt cttccgcaaa ctgcaatccg cgcatggtgc gcacattatt gatgcggtta
     6361 actgcgcggg aaccaaggtc agagctgaat tcctgatcgc ggaagacggt taaaccttct
     6421 ttcaggctga gctggaacca gtcgcggcag gtcactcggt taccggtcca gttatggaaa
     6481 tattcatggc cgataacgcg ttcaatatcg aggtaatctt tgtcggtggc ggtgtcggtg
     6541 cgggccagca catatttgga gttaaagata ttcagaccct tattctccat tgcgcccata
     6601 ttgaagaaat ccaccgcgac gatcatatag atgtcgaggt catactccag gccgaagcgt
     6661 tcttcatccc atttcattga gtttttcagc gaggtcatcg cccacggcgc gcgatcaagg
     6721 ttgccgcgat cgacgtacag ctccagcgcc acttcgcgac cagaacgcgt ggtaaaggtg
     6781 tcgcgcagta catcaaagtc gcctgccacc agcgcaaaca ggtagcacgg tttcgggaac
     6841 gggtcctgcc actgtaccca atggcgtccg ttttccagtt caccttgcgc aacgcggtta
     6901 ccgttggaaa gcaggaaggg atatttggtt ttatcggcaa taattttggt ggtaaaacgc
     6961 gccagcacgt ccgggcggtc gagataatac gtaatatggc ggaaaccttc ggcttcacac
     7021 tgggtgcaaa gcgcatcgcc tgactgataa agcccttcca gcgcggtatt cgccgccggg
     7081 cttatttcat taacgatctt aagcgtaaaa tgctccggca aattactgat gaccagtgcg
     7141 ccctcttctt ctttccaggc ggtccacggc tcatcattaa tatgaacaga aaccagtttg
     7201 aggtcttcgc cgttgagacg aagtggagca tctgatgcac catgacggac agcctggctg
     7261 accgcggtaa cgaccgtctt ttgcgcgtcg aggtcaaagg tcaagtcaat atcagtaatc
     7321 tggtaatccg gcgcacgata atcgtgacgg tatttggctt gtggctgttg agtcataaat
     7381 aacctttagc atcttttata gagtctggtg ttcagtctat tcctgttgcg taaatcgcgc
     7441 tatgcagaat cttcatcttt tcaggtacaa acgcctttat tgctacattt ttataacata
     7501 caccgcgtaa tgccatcgac cagaaaggtg gcatatggtg tgatcggggt tcaataaatt
     7561 gcgaaacaag gtatactcca gcagttcctg aagatgttta ttgtactaaa cgctcctgta
     7621 cgaggacgct actgcgcacc tatgacacaa ttcgcttctc ctgttctgca ctcgttgctg
     7681 gatacagatg cttataagtt gcatatgcag caagccgtgt ttcatcacta ttacgatgtg
     7741 catgtcgcgg cggagtttcg ttgccgaggt gacgatctgc tgggtattta tgccgatgct
     7801 attcgtgaac agattcaggc gatgcagcac ctgcgcctgc aggatgatga atatcagtgg
     7861 ctctctgccc tgcctttctt taaggccgac tatcttaact ggttacgcga gttccgcttt
     7921 aacccggaac aagtcaccgt gtccaacgat aatggcaagc tggatattcg cttaagcggt
     7981 ccgtggcgtg aagtcatcct ctgggaagtt cctttgctgg cggttatcag tgaaatggta
     8041 catcgctatc gctcaccgca ggccgacgtt gcgcaagccc tcgacacgct ggaaagcaaa
     8101 ttagtcgact tctcggcgtt aaccgccggt cttgatatgt cgcgcttcca tctgatggat
     8161 tttggcaccc gtcgccgttt ttctcgcgaa gtacaagaaa ccatcgttaa gcgtctgcaa
     8221 caggaatcct ggttcgtggg caccagcaac tacgatctgg cgcgtcggct ttccctcacg
     8281 ccgatgggaa cacaggcaca cgaatggttc caggcacatc agcaaatcag cccggatcta
     8341 gccaacagcc agcgagctgc acttgctgcc tggctggaag agtatcccga ccaacttggc
     8401 attgcattaa ccgactgcat cactatggat gctttcctgc gtgatttcgg tgtcaagttc
     8461 gctagtcggt atcagggcct gcgtcatgac tctggcgacc cggttgaatg gggtgaaaaa
     8521 gccattgcac attatgaaaa gctgggaatt gatccacaga gtaaaacgct ggttttctct
     8581 gacaatctgg atttacgcaa agcggttgag ctataccgcc acttctcttc ccgcgtgcaa
     8641 ttaagttttg gtattgggac tcgcctgacc tgcgatatcc cccaggtaaa acccctgaat
     8701 attgtgatta agttggtaga gtgtaacggt aaaccggtgg cgaaactttc tgacagccct
     8761 ggcaaaacta tctgccatga taaagcgttt gttcgggcgc tgcgcaaagc gttcgacctt
     8821 ccgcatatta aaaaagccag ttaatatcat cagggagcta atcggctccc tttttttacc
     8881 tttaattccg aaatctttcg ctgcatttgc gaattctgct tgtctgattg cagatgccag
     8941 gtaacatagg tatccccccc atttacgggt ggacaagtgt ttattttttc cgactattaa
     9001 cagagagaat attatgagcg ttgtgcctgt agccgacgta ctccagggcc gcgtagccgt
     9061 tgacagcgaa gtcaccgtac gcggatgggt acgtacccgc cgagattcaa aagctggcat
     9121 ctccttcctc gccgtttatg acggttcctg ctttgatcct gtacaggctg tcatcaataa
     9181 ttctctgccc aattacaatg aagacgtcct gcgtctgacc accggctgct cggtcattgt
     9241 gacgggtaaa gtcgtggcat cgccgggcca ggggcaacaa tttgaaattc aggccagcaa
     9301 ggttgaagtt gctggttggg ttgaagatcc agacacttac ccgatggcgg caaaacgcca
     9361 cagcattgag tatctgcgtg aagtcgctca cctgcgtccg cgcacaaacc tgattggtgc
     9421 cgtcgcgcgc gttcgccata cgctggcgca ggcgctgcat cgcttcttta acgagcaggg
     9481 attcttctgg gtttcaacgc cactgattac cgcatctgat accgaaggtg caggcgaaat
     9541 gttccgcgtt tctacgctgg atctggaaaa cctgccgcgt aacgatcagg gcaaagtgga
     9601 tttcgacaaa gacttctttg gtaaagagtc tttcctgacc gtatctggcc agttgaacgg
     9661 cgaaacctac gcttgcgcat tatccaaaat ttataccttc ggcccgactt tccgtgctga
     9721 aaactccaac accagccgtc acctggcgga attctggatg ctggagccgg aagtggcgtt
     9781 tgctaacctg aacgatatcg cgggtctggc tgaagccatg ctgaaatatg tcttcaaagc
     9841 ggttctcgaa gaacgcgcag acgacatgaa attcttcgct gaacgcgtag ataaagatgc
     9901 cgtttcacgt ctggaacgct tcattgaggc cgatttcgcg caggtggatt acaccgacgc
     9961 agtgaccatt ctcgaaaact gcggcaggaa gtttgaaaac ccggtttact ggggcgtcga
    10021 tctctcttct gagcatgagc gttatctggc ggaagaacac tttaaagcac cggtagtggt
    10081 taaaaactat ccgaaagata ttaaagcgtt ctatatgcgc ctcaacgaag acggtaaaac
    10141 cgttgcggct atggacgttc tggctccggg catcggtgag atcattggtg gctcccagcg
    10201 tgaagaacgt ctggacgttc tggacgagcg tatgctggaa atgggcctga ataaagaaga
    10261 ttactggtgg tatcgcgatc tgcgtcgcta cggtactgtt ccgcattcag gtttcggtct
    10321 tggttttgaa cgcctgattg cttacgtaac tggcgtgcaa aacgtgcgtg atgtgattcc
    10381 gttcccacgt actccgcgca acgccagctt ctgattttct tcctcaaaga gccagcgcct
    10441 gctggctctt ttttatcctt tctgtcaagt tatctgtttg ttaagtcaag caatctattt
    10501 gcaaccccgc cataacgatc atcctgttac ggaatattac attgcaacat ttacgcacaa
    10561 aaaataaccc gcattcttat tgcggattag ttttttctta gctgatagca caattttcat
    10621 actatttttt ggcattctgg atgtctgaaa gaggattttg tgccaggtcg ataaagtttc
    10681 catca
//
LOCUS       DAZPLI010000135        10537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZPLI010000135 DAZPLI010000000
VERSION     DAZPLI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10537
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.136"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..656
                     /locus_tag="ACHUU3_003309"
     CDS             <1..656
                     /locus_tag="ACHUU3_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR2083305.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLAIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            614..1291
                     /gene="bglJ"
                     /locus_tag="ACHUU3_003310"
     CDS             614..1291
                     /gene="bglJ"
                     /locus_tag="ACHUU3_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR2083306.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1329..2117)
                     /gene="fhuF"
                     /locus_tag="ACHUU3_003311"
     CDS             complement(1329..2117)
                     /gene="fhuF"
                     /locus_tag="ACHUU3_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR2083307.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2258..2494
                     /locus_tag="ACHUU3_003312"
     CDS             2258..2494
                     /locus_tag="ACHUU3_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR2083308.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            complement(2533..2619)
                     /locus_tag="ACHUU3_003313"
     tRNA            complement(2533..2619)
                     /locus_tag="ACHUU3_003313"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2583..2585),aa:Leu,seq:cag)
     gene            complement(2887..3918)
                     /gene="rsmC"
                     /locus_tag="ACHUU3_003314"
     CDS             complement(2887..3918)
                     /gene="rsmC"
                     /locus_tag="ACHUU3_003314"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR2083309.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4021..4434
                     /gene="holD"
                     /locus_tag="ACHUU3_003315"
     CDS             4021..4434
                     /gene="holD"
                     /locus_tag="ACHUU3_003315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR2083310.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4403..4849
                     /gene="rimI"
                     /locus_tag="ACHUU3_003316"
     CDS             4403..4849
                     /gene="rimI"
                     /locus_tag="ACHUU3_003316"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR2083311.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            4864..5541
                     /gene="yjjG"
                     /locus_tag="ACHUU3_003317"
     CDS             4864..5541
                     /gene="yjjG"
                     /locus_tag="ACHUU3_003317"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR2083312.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5632..7221
                     /gene="prfC"
                     /locus_tag="ACHUU3_003318"
     CDS             5632..7221
                     /gene="prfC"
                     /locus_tag="ACHUU3_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR2083313.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7614..8219
                     /gene="osmY"
                     /locus_tag="ACHUU3_003319"
     CDS             7614..8219
                     /gene="osmY"
                     /locus_tag="ACHUU3_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR2083314.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8346..8507
                     /locus_tag="ACHUU3_003320"
     CDS             8346..8507
                     /locus_tag="ACHUU3_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR2083315.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8629..9702
                     /locus_tag="ACHUU3_003321"
     CDS             8629..9702
                     /locus_tag="ACHUU3_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR2083316.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            9699..10481
                     /locus_tag="ACHUU3_003322"
     CDS             9699..10481
                     /locus_tag="ACHUU3_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR2083317.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         2566 a         2628 c         2819 g         2524 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc aattcacagc aacgctgaaa
      541 tgtatgcatg gataaatagc gcgcagggtg caagagaact taacttgcct tctgtttatg
      601 gagatgccgc agaatggaac acagccgaat taagaagaga aatgtcgcac tcatagaaaa
      661 atgcgtcatg agcagtatcg gtattgagag tttattcaga aagtttgcgg gtaaccctta
      721 taagctccat acctatacca gtcaggagtc atttcaggat gccatgtcgc ggatctcatt
      781 tgcggcggtc attttttctt tttctgccat gagaagtgag cgcagagagg gattatcttg
      841 cctgactgaa ctggcgatta agtttccgcg tacccggcgt ttagttattg cggatgatga
      901 tattgaagct cggctgattg gttcattgtc gccatcaccg ctggacggtg tattaagtaa
      961 agcgtcaacg ctggagattt ttcatcagga actctttttg tcattaaatg gtgtacgtca
     1021 ggcgaccgac cgactgaaca atcagtggta cattaaccaa agccggacgc taagcccgac
     1081 ggagagagaa atattgcgct ttatgtcgcg tggctactca atgacacaaa ttgccgagca
     1141 gcttaaacgc aatatcaaaa cgatccgtgc acataaattt aatgtgatgt cgaaactggg
     1201 cgtcagttct gatgcagggt tgttggaggc cgcagatatt ctgttatgta tgcggcattg
     1261 cgaagcaagt aatgtgttgc atccctatta atccgcatga tgccgggttt acttccccgg
     1321 cagtgctttc atttcagcgt acaatcgcca cattgctgca catccggtaa gcgataacgc
     1381 tggcagcagg ttcggcgcac cagcaggcca tcgcgcagca cgacggtacg ccagagtgga
     1441 ttatcttcac cgttcgtgag cgttttctca aaaaagaggg catggcgcag cgattcaacc
     1501 gtcgcctcgc cgagcagttg cttcatctca gtgagatacc agttgatcaa ataaccggta
     1561 ttactccaga taagtttgcc gttgatctct ccggtcgctt ctagtgcctg cacaaccgga
     1621 accagcgcct ggctgattaa cgtttccatt cgctgctgcg gcgaatgtgg tgttgcgttt
     1681 ttatcttcac acacatcgac ccagaaacag gcgacgcgtc cggtttcgtg aaactcagca
     1741 tggaaatgtt ccggcgacac atctaatgcc ttttcctgcg tcagtagcgc cagcattaat
     1801 ggtggcacca tcaggccgat atacaattgt gcccatagtg agatcagcgg tttgttctcg
     1861 cggatcatcg ttggttggtt gcgatagata tgatcggaat agaccgcgag tagagaactt
     1921 agcgcattcg gtgatgacca ttgcgccagc gtcatggcgt taagcggagc gggttcatcc
     1981 aggcggataa actcgagcaa atgttcacga tgtttggcga tcgtcgcacg tacggcttgt
     2041 gcaagcccag catcctgcgg ctggagatgc gttcgccaga tgatatcttc atagagcggt
     2101 gcggaacgat aggccataat cgggatagta atctaaatga taatgattgc taatcctagc
     2161 gataggttta cccgatagca agggatttct ctggcttgca aatgataaaa attatcatat
     2221 gatattggtt atcattatca atgaaagaga tgaaaccatg ttgcaacgta cgctgggcag
     2281 tggctgggga gtgttgctgc cgggattgct gattgcaggg ctgatgtatg cggatttatc
     2341 gccggatcag tggcggattg tcattctgat gggattagta ttgacgccgg tgatgctgta
     2401 tcacaaacag ttacggcatt acgttttgct accatcgtgc ctggcgctta ttgctgccat
     2461 catgctgatg ataatgaatt tgaatcaggg atgaaaaatc aaggaagaaa caagaaagga
     2521 agtaaagata attggtgcga ggggggggac ttgaaccccc acgtccgtaa ggacactaac
     2581 acctgaagct agcgcgtcta ccaattccgc caccttcgca taccatcaat tcttaaaaag
     2641 aattgctacc acggaggcgc attctagtgg ttttcagctt ttcgtcaata gttaattatc
     2701 gccggaggtg taattgctgg aaaaatgtcc atcaggaaac tagcgtgcag gtttggtagg
     2761 catgcggggg cagatgccag atgcgacgct ggcgcgtctt atctggccta cgaagggcta
     2821 acgtgcaggt tttgtaggtc ggataaggcg ttcacgccgc atccgacacg gtattcggcg
     2881 agataattaa cctttcttcg cctggcgggt cataatggcg cgatacacct tgaaacgccc
     2941 ggtttgcgca atcacttcat ggaagccaaa tgtctcatcc agcacgtccg ggtaaggcag
     3001 gaaggcgttc gctacaattc gcagctcgcc gccgctatta agatgacgca ccgcgccgcg
     3061 aatcagcgtt tgcgccgcat ccaggctggt ttgcatccca tcgtggaacg gcgggttgga
     3121 gatgatcata tcaaaacgac ctttcacctc ggaaaagacg ttgctggcaa agacttcacc
     3181 ttcaacacag ttggccgcaa gtgttgcgcg gctggcttct accgctggcg cagagacatc
     3241 gcacaaggtg agacgaattt tcggcgaatg gcgcgcaaag gcaactgaaa gcacccccgc
     3301 gccacagccg acatccagca ctttaccttt cgtgtgcgga gttaacgtcg agagcagcaa
     3361 ctggctaccg acatccagac cgtcgcggct aaacacgcca ggcagcgttt tgaccgtcag
     3421 gccatcgacg ctgtattcgc cccagaattt atcggcatca aataccggct gtttttccag
     3481 acgaccaaaa tagaggccac agcgacgagc gctgtcgact ttattcaacg gcgcataatc
     3541 tgccagcatc tgctcggcgc tgcgcacgcc gctgcggttc tcgccaacga caaaaatatc
     3601 tgtccccact ggcagcagag aaagtaaatt catcaactgg aactgggctt ccggtttgtt
     3661 cttcggccag tagtaaatca gcgtatcgca atctgcgacg tcatccaccg tggcgaccag
     3721 actgaaacgg gcgttatccc ccatctggcg gcttaatacc tgccagtggt ggaattgctg
     3781 ggtatgagca cggctggccg cggtatctaa acgcgcgggc aggtcatcct gtaagtctcc
     3841 ggcaaacaga atacggcttt gttcgaaatc atcactgtga cgcagcaaga cttcacttgc
     3901 cggggtaaat gcagacatgg aatgctcctc aattgatact ggcggcgatt atagccatat
     3961 gttggcgcgg tatcgacgaa tttgctatat ttgcgcccct gacaacagga gcgattcgct
     4021 atgacatccc gacgagactg gcagttacag caactgggca ttacccagtg gtcgctgcgt
     4081 cgccctggcg cgttgcaggg ggagattgcc attgcgatcc cggcacacgt ccgtctggtg
     4141 atggtggcaa acgatcttcc cgccctgacc gatcctttag tgagcgatgt tctgcgcgca
     4201 ttaaccgtca gccccgatca agtgctgcaa ctgacgccag aaaaaatcgc gatgctgccg
     4261 caaggcagtc gctgcaacag ctggcggttg ggtactgacg aaccgctatc actggaaggc
     4321 gctcaggtgg catcaccggc gctcaccgaa ttacgggcaa acccaacggc acgcgccgcg
     4381 ttatggcaac agatttgcac atatgaacac gatttcttcc ctcgaaacga ctgatttacc
     4441 agcggcttac cacattgaac aacgcgccca cgcctttccg tggagtgaaa aaacctttgc
     4501 cagcaaccag ggcgagcgtt atctcaactt tcagttaacg caaaacggca aaatggcggc
     4561 gtttgcgatt acgcaagtgg tgctggatga agctacattg ttcaatattg cggtcgatcc
     4621 tgactatcag cgtcagggat tgggaagggt gctgctggaa catctgatcg acgaactgga
     4681 aaaacgcggc gtggcgacac tatggctgga agtccgtgcc tcaaatgctg ccgccattgc
     4741 cctgtacgaa agtttaggct ttaacgaggc gacgattcgc cgcaattact accccaccac
     4801 ggacggtcgc gaagacgcca tcatcatggc gttgccaatc agtatgtaag acaaggtgga
     4861 ataatgaagt gggactggat tttctttgat gccgatgaaa cgctgtttac ctttgactca
     4921 ttcaccggcc tgcagcggat gtttcttgat tacagcgtca cctttaccgc tgaagatttt
     4981 caggactatc aggccgttaa caagccactg tgggtggatt atcaaaacgg cgcgatcact
     5041 tcattacagc ttcagcacgg gcgttttgag agctgggccg aacggctgaa agttgaagca
     5101 ggcttgctta acgatgcctt tattaatgcg atggcggaaa tctgcacgcc gctgccgggc
     5161 gcggtttctc tgcttaacgc cattcgtggc aacgccaaaa tcggcatcat caccaacggc
     5221 tttagcgcct tgcagcaagt gcgtctggaa cgcacgggcc tgcgtgatta cttcgatttg
     5281 ctggtgattt ccgaagaagt tggcgttgcc aaaccgaata agaaaatttt cgattatgcg
     5341 ctggaacagg cgggcaatcc tgaccgttca cgcgtgctga tggttggcga cactgccgag
     5401 tccgatattc tcggtggcat caacgccggg cttgcgacct gctggctgaa tgcgcacaat
     5461 cgcgagcaac cagaaggcat cgcgcccacc tggaccgttt cttcgttgca cgaactggag
     5521 cagctcctgt gtaaacactg attgcctccc ccccgttgat gggtaaaata gccgcaattt
     5581 ttcgttttca acaagcgcgg cgcgatgccg cttactcaag aagaaagaat tatgacgttg
     5641 tctccttatt tgcaagaggt ggcgaagcgc cgcacttttg ccattatttc tcacccggat
     5701 gccggtaaga ctaccatcac cgagaaggtg ctgctgttcg gacaggccat tcagaccgcc
     5761 ggtacagtaa aaggccgtgg ttccaaccag cacgctaagt cggactggat ggagatggaa
     5821 aagcagcgtg ggatctccat tactacgtct gtgatgcagt ttccgtatca cgattgcctg
     5881 gttaacctgc tcgacacccc ggggcacgaa gacttctcgg aagataccta tcgtaccctg
     5941 acggcggtgg actgctgcct gatggttatc gacgctgcaa aaggtgttga agatcgtacc
     6001 cgtaagctga tggaagttac ccgtctgcgc gacacgccga tcctcacctt tatgaacaaa
     6061 cttgaccgtg atatccgcga cccgatggaa ctgctcgatg aagttgagaa cgagctgaaa
     6121 atcggctgcg caccgatcac ctggccgatt ggctgcggca agctgtttaa aggcgtttac
     6181 cacctttata aagacgaaac ctatctctat cagagcggta aaggccacac gattcaggaa
     6241 gtccgtattg ttaaagggct gaataacccg gatctcgacg ctgcggttgg tgaagatctg
     6301 gcacagcagc tacgtgacga actggaactg gtgaaaggcg cgtctaacga gttcgacaaa
     6361 gaattgttcc ttgcgggcga aatcactccg gtattcttcg gtactgcgct gggtaacttc
     6421 ggcgttgatc atatgctgga tggcctggtg gagtgggctc ctgcgccgat gccgcgtcag
     6481 actgataccc gtaccgtaga ggcgagcgaa gacaaattta ccggcttcgt atttaaaatt
     6541 caggccaaca tggacccgaa acaccgcgac cgcgtggcgt ttatgcgcgt agtatccggt
     6601 aaatatgaaa aaggcatgaa gctgcgccag gtgcgtactg cgaaagatgt ggttatctcc
     6661 gacgcgctga cctttatggc gggcgaccgt tcgcacgttg aagaagcgta tccgggcgat
     6721 atcctcggcc tgcacaacca cggcactatt cagatcggcg acacctttac ccagggtgag
     6781 atgatgaagt tcaccggtat tccgaacttc gcaccagaac tgttccgtcg tatccgcctg
     6841 aaagatccgc tgaagcaaaa acagctgctc aaagggctgg tacagctttc cgaagagggc
     6901 gcggtgcagg tgttccgtcc gatctccaac aacgatttga tcgttggtgc tgttggtgtg
     6961 ctgcagtttg atgtggtggt atcgcgcctg aagagcgaat acagcgttga agcagtatat
     7021 gaatcagtca acgttgccac tgcccgctgg gtagaatgtg cggacgcgaa gaaattcgaa
     7081 gagttcaagc gtaagaacga aagccaactg gcgcttgatg gcggcgataa cctcgcttac
     7141 atcgctacca gcatggtcaa cctgcgcctg gcgcaggaac gttatcccga cgttcagttc
     7201 caccagaccc gcgagcatta attcttgcct ccagggcgcg gtagccgctg cgccctgtca
     7261 atttcccttc cttattagcc gcttacggaa tgttcttaaa acattcactt ttgcttatgt
     7321 tttcgctgat atcccgagcg gtttcaaaat tgtgatctat atttaacaaa gtaatgacat
     7381 ttctgacggc gttaaatacc gttcaatgcg tagatatcag tatctaaagc cgtcgattgt
     7441 cattctaccg atattaataa ctgattcaga ggctgtaatg gtcgttattc atcacccatc
     7501 gcttttgtga tggcgaccat tgacttctgt agagggtgaa gtctctccct attcagcaat
     7561 gcaacctcgt gttgccaggc tcaaattacg agcaaacata caggaataaa tcgatgacta
     7621 tgacaagact gaagatttcg aaaactctgc tggctgtaat gttgacctct gccgtcgcga
     7681 ccggctctgc ctacgcggaa aacaacgcac agactaccaa tgaaagcgca gggcaaaaag
     7741 tcgatagctc tatgaataaa gtcggtaatt tcatggatga cagcgccatc accgcgaaag
     7801 tgaaggcggc gctggtggat catgacaaca tcaagagcac cgatatctct gtcaaaaccg
     7861 atcaaaaagt cgtgaccctg agcggtttcg ttgaaagcca ggctcaggcc gaagaggcag
     7921 tgaaagtggc gaaaggcgtt gaaggggtga cctctgtcag cgacaaactg cacgttcgcg
     7981 acgctaaaga aggctctgtg aagggctacg cgggtgacac cgccaccacc agtgaaatca
     8041 aagccaaact gctggcggac gatatcgtcc cttcccgtca tgtgaaagtt gaaaccaccg
     8101 acggcgtggt tcagctctcc ggtaccgtcg attcccaggc acagagcgac cgtgctgaaa
     8161 gtatcgccaa agcggtagat ggtgtgaaaa gcgttaaaaa tgatctgaaa actaagtaat
     8221 tcgtcgtaat tcgtcctccc gaactttgtc gggaggcgcg atgtgcacca cactaaaaat
     8281 atcgcattga gtgattttca cgctcatatc aagcggtgac attaactatg gtaaaggaga
     8341 cgcttatgtt tcgttggggc atcatatttc tggttatcgc gttaatcgcc gccgcacttg
     8401 ggtttggtgg tctggccggt accgctgcag gcgcagctaa aattgtcttt gtcgtcggga
     8461 ttattctgtt cctggtgagt ttgttcatgg gccgaaaacg accctagatt tcagaccatt
     8521 aagaaaattt tatccaaagc cagtccagcg gactggcttt tgcggtttta gcgaataata
     8581 aattgcgtta ctttgtcatt ctttcacaac aaaaacagga aggcagaggt ggggcagcga
     8641 atacctgtaa cgcttggtaa tattgcgccg ttgtcgctaa ggccgttcca gcctggacga
     8701 atagctctgg tgtgcgaagg cggcggacag cgtggaattt tcacggctgg cgtgctggat
     8761 gagtttatgc gcgcgcagtt taatcctttc gatctttatc tcggcacatc tgccggggcg
     8821 cagaacctct cggcgtttat ctgcaatcag cccggttacg cgcgcaaagt catcatgcgc
     8881 tataccacaa aacgcgaatt tttcgatcca ttgcgctttg tccgtggagg aaatcttatc
     8941 gatctcgact ggctggtgga ggccaccgca agccagatgc cgttgcaaat ggataccgcc
     9001 gcgcggttgt ttgacagcgg caaatcgttt tatatgtgcg cctgtcgtca ggatgactac
     9061 gcgcctaatt actttttacc aaccaaacaa aactggctgg atgtgattcg cgcctccagt
     9121 gcgatacctg gcttttatcg tagcggagtg tcgctggaag gcattaacta cctggatggc
     9181 gggatcagtg atgcgattcc ggttaaagag gcggcaaggc agggcgctaa aacgttggtc
     9241 gtcattcgca ctgtgccgtc acaaatgtac tacacgccgc agtggttcaa acgcatggaa
     9301 cgctggctgg gtgacagtag cctgcagccg ctggtcaatc tggtgcagca tcatgaaacc
     9361 agctatcgtg acattcagca atttattgag aaaccaccgg gcaagctgcg gatattcgaa
     9421 atttatccgc cgaagccatt acatagtatc gcgcttggca gtcggattcc ggcgctgcgt
     9481 gaagactata aacttgggcg tttatgcggt cgttatttcc tcgccacggt tggcaagcta
     9541 ttaactgaaa aagcgccgct tacccgccat ctggtgccag tggtgacgcc ggaatcgatc
     9601 gttattccgc ctgcgccagt cgccaacgat acgctggttg ccgaagtgag cgatgctccg
     9661 caggcgaacg acccgacatt taacaatgag gatctggctt gatttgccgt tttatcgata
     9721 cccactgcca ttttgatttc ccgccgttta gtggcgatga agaggccagc ctgcaacgcg
     9781 cggcacaagc gggcgtaggc aagatcattg ttccggcgac agaggcggaa aattttgccc
     9841 gcgtgcaggc gttagcggaa aaatatcaac cgctgtatgc cgcattgggg ttgcatccgg
     9901 ggatgttgga aaaacatagc gatgtgtctc ttgatcagct acagcaggcg ctggaaaggc
     9961 gtccggcgaa ggtggtggcg gtgggggaga tcggtctgga tctcttcggc gacgatccgc
    10021 aatttgagag gcagcagtgg ttactcgacg aacagctgaa actggcgaaa cgctacgatc
    10081 tgccggtgat ccttcattcc cggcgtatac acgacaaact ggcgatgcat cttaaacgtc
    10141 acgatttacc gtgcactggc gtggttcacg gtttttccgg cagcctgcaa caggccgagc
    10201 ggtttgtaca gctgggctac aaaatcggcg taggcggtac tatcacctat ccacgcgcca
    10261 gtaaaacccg cgatgtcatc gcaaaattac cgctggcatc gttgttgctg gaaaccgacg
    10321 cgccggatat gccgctcaac ggctttcagg ggcagcctaa ccgaccggaa caggctgtgc
    10381 gggtgtttga cgtgctttgc gagttgcgcc cggaaccaga agatgagatt gccgaagtgt
    10441 tgcttaataa tacgtatgcg ttgtttagcg ttagtgggta gcctaccctt cgtaggcagc
    10501 atccggcagt cggcgcataa tgcctgatgc gacgctt
//
LOCUS       DAZPLI010000136        10532 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZPLI010000136 DAZPLI010000000
VERSION     DAZPLI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.137"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(71..1099)
                     /gene="malI"
                     /locus_tag="ACHUU3_003323"
     CDS             complement(71..1099)
                     /gene="malI"
                     /locus_tag="ACHUU3_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR2083318.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1274..2866
                     /gene="malX"
                     /locus_tag="ACHUU3_003324"
     CDS             1274..2866
                     /gene="malX"
                     /locus_tag="ACHUU3_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR2083319.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2876..4048
                     /gene="malY"
                     /locus_tag="ACHUU3_003325"
     CDS             2876..4048
                     /gene="malY"
                     /locus_tag="ACHUU3_003325"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR2083320.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4152..5153
                     /gene="add"
                     /locus_tag="ACHUU3_003326"
     CDS             4152..5153
                     /gene="add"
                     /locus_tag="ACHUU3_003326"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR2083321.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5189..6229)
                     /locus_tag="ACHUU3_003327"
                     /pseudo
     CDS             complement(5189..6229)
                     /locus_tag="ACHUU3_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            6473..6598
                     /gene="blr"
                     /locus_tag="ACHUU3_003328"
     CDS             6473..6598
                     /gene="blr"
                     /locus_tag="ACHUU3_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR2083322.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6863..7078
                     /gene="ydgT"
                     /locus_tag="ACHUU3_003329"
     CDS             6863..7078
                     /gene="ydgT"
                     /locus_tag="ACHUU3_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR2083323.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7164..7604
                     /locus_tag="ACHUU3_003330"
     CDS             7164..7604
                     /locus_tag="ACHUU3_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR2083324.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7681..8262
                     /gene="rsxA"
                     /locus_tag="ACHUU3_003331"
     CDS             7681..8262
                     /gene="rsxA"
                     /locus_tag="ACHUU3_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR2083325.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8262..8840
                     /gene="rsxB"
                     /locus_tag="ACHUU3_003332"
     CDS             8262..8840
                     /gene="rsxB"
                     /locus_tag="ACHUU3_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR2083326.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8833..>10532
                     /gene="rsxC"
                     /locus_tag="ACHUU3_003333"
     CDS             8833..>10532
                     /gene="rsxC"
                     /locus_tag="ACHUU3_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR2083327.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     "
BASE COUNT         2406 a         2723 c         2694 g         2709 t
ORIGIN      
        1 ctgtgtgcca cattttttat cattaacctt gaggaaagga cactaccagg tatagcagat
       61 aaagagagga ttatttcgct gcaatgagcc gggcgggaat aataagattg cgtgaatgcg
      121 tctcttcatg ggtgattttt tgcatcatgc gatccgcaag tgtggtacca agttcccgcg
      181 caggcgtgct ggcccaggta acgggtatat catcaagtgt ggttggtgtc gcatcggtaa
      241 atgccgccag cgaaacttgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc
      301 gccctgcttt cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca
      361 cagcactgat ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct
      421 ggctggatgt gcactccagc acccaatcgc tgtgaaacgg caggccaaat tttagtagag
      481 ttgcacaata gcctcccacc cgttctgccc gggttaatga ggaactttgc cctcccagcc
      541 aggcgatccg ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca
      601 tgttgtccgg gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca
      661 ccgggatagc tttttcttct gccatccgtc gcaggtcatc gctgcttcct gcagccccgg
      721 caattaccac accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct
      781 cgccgtcttt accgccgtga agcaaaaaaa ccatccgtcc ctgcgcttcc agagcttccg
      841 tcaatccggc cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa
      901 tgacgccgct ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct
      961 cttcaatggc ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccactaa
     1021 gcaccagcga aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta
     1081 tttttttggc ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt
     1141 ttacctttta tttatcttat acccgctatt atcgttgcgt aatgtgattt atgcttcact
     1201 aaaatttgat aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac
     1261 gaggagtcgt tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca
     1321 gcagttaggc aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct
     1381 cggcattggt agttctctta gtagccacga tgtcatcacc ctgatcccgg tcctgggcaa
     1441 ccccgtgttg caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt
     1501 cctgccagtc atgttctgta tcgccatccc gctgggtctg gcacgcgaaa acaaaggcgt
     1561 agcggcattc gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt
     1621 gaccaataaa ggcattctgc caaccacgga cgccgcggtt ctgaaagcca ataacatcca
     1681 gagcattctt gggatccagt cgatcgacac cgggatcctc ggtgcggtga tcgccggtat
     1741 tatcgtctgg atgctgcatg agcgattcca taatatccgc ctgccggatg cgcttgcatt
     1801 cttcggcggt acgcgcttcg taccaatcat ctcctcgctg gtgatgggcc ttgtcggcct
     1861 ggtgattcca ttagtctggc cgattttcgc gatgggtatt agcggcttgg gccatatgat
     1921 aaacagcgcg ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc
     1981 gtttggtctg catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca
     2041 ggaagtctgc ggtcaaaccg tcagcggcgc attgaccatc ttccaggcgc aattgagttg
     2101 cccgaccact cacggttttt ctgaaagcgc cacgcgtttc ctttcgcaag gcaaaatgcc
     2161 tgcgtttctc ggcggtctgc caggtgcagc gttagcgatg tatcactgcg cgcgcccgga
     2221 aaatcgccat aaaattaaag ggctgctgat ttctggcctg atcgcctgtg tcgttggcgg
     2281 cacgaccgaa ccgctggaat tcctgttcct gttcgtggcg ccagttctgt atgtcatcca
     2341 cgcgctgtta accggcctcg gcttcactgt catgtctgtg ctcggcgtca ccatcggtaa
     2401 taccgacggc aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa
     2461 gtggtacatg gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg
     2521 tttcgctatc acccgcttca atctgaaaac cccggggcgc gatagcgaag tggccagttc
     2581 aatcgaaaaa gccgttgccg gtgcgccggg taaatcaggt tacaacgttc cggcaatcct
     2641 cgaagcctta ggcggtgccg acaatattgt tagcctcgat aactgcatta cccgtctgcg
     2701 tttgtctgtg aaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat
     2761 tggcgtagta caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc
     2821 agtaaaagat gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt
     2881 cgatttttca aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc
     2941 tgaccgtttc ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac
     3001 tgccccctgc attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag
     3061 ccgctggaaa aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta
     3121 caccgcaatt gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga
     3181 actgattcgt cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga
     3241 cgcattttac aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa
     3301 gcaggctgac ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga
     3361 atgtaaaatt atgctcctgt gtagcccaca gaatcctacc gggaaagtgt ggacgtgcga
     3421 tgagctggag atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga
     3481 aatccatatg gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg
     3541 gggagactgg gcgttgctga cgtcgggatc gaaaagtttc aatattcccg cgctgaccgg
     3601 tgcttacggg attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg
     3661 acgtgatggg ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca
     3721 gcaaggtgca ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat
     3781 ctcagataaa atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta
     3841 tctggcctgg cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct
     3901 tatcgagcaa gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg
     3961 ttttgtccgt ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt
     4021 aataaacgcc atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa atcgttgcgc
     4081 aatcgtggat ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa
     4141 agagtgcgac catgattgat accaccctgc cattaactga tatccatcgc caccttgatg
     4201 gcaacattcg tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg
     4261 cacaatccct ggaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg
     4321 tgagctttct gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc
     4381 gccgcgtggc atttgaaaac attgaagatg cagcccgtaa cggcctgcac tatgtcgagc
     4441 tgcgtttttc accaggctac atggcaatgg cacatcagct gcctgtagcg ggtgttgtcg
     4501 aagcggtgat cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta
     4561 tcggcattat gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt
     4621 tagcccaccg tgaccagatt accgcacttg atttagctgg tgatgaactt ggattcccgg
     4681 gaagtctgtt cctttctcac ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc
     4741 atgcaggcga agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg
     4801 agcgtattgg acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg
     4861 agcaacaaat tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag
     4921 agctggctgc acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca
     4981 ctgacgatcc cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag
     5041 ctgctgggtt atcccgtgag caaatccgcc aggcacagat taatggtctg gaaatggctt
     5101 tcctcagcgc agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat
     5161 ggatggtgca atttgcacca tccatttttc acgcaaggca caacgtcgcg cgatgtttgg
     5221 cggattcgat gcccagctca atcaactcca ttacctggat tgcctagctt gccggaaccg
     5281 gattttcacc atcgccattt aacgcatcac gaatcgccgc atagtaagcc ggataattcc
     5341 caggcaccgt caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc
     5401 catcacgcat atcgtagccc cagtcttcct gcggcagacg ctcgccattt ttcagacgtt
     5461 cttcctgtgg atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc
     5521 gtgctgactc agcggctgcc agcatggtac cgtgtaaaat gactcgccgc tgtggatagg
     5581 acaagatggc gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta
     5641 ccgtcatgct gaccggtaaa ccaaatagcg taatggcctg atcaagaaga tgcggtgcta
     5701 aatcgtacca gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgcacctgcg
     5761 gacggaagcg gtcaaaatga gactcaaagt aagcaacttc acccagcacg ccttccgcga
     5821 gcaaaccttt tagcgtcaag aaatcgctat cccaacgacg gttatggaat acagacagca
     5881 cacgccccag gctttttgcc agcgcatcca gctctcgcgc ttgtgacagt gtcacggtaa
     5941 agggtttatc aacgaccaca tgtttacccg cctcaagcgc cgctttggct aacgggaaat
     6001 gggtatcgtt gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct
     6061 tcggctcaga gacaaccgta accgttggcc agtcggcttt tacttttgtt tcatcactgc
     6121 tggagattac cgccagttcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt
     6181 tgctcgcata accataccca atcaacccaa cacggatgtt gtcgctcatg tcattgcctc
     6241 tcattgaagt atgatggcta tttgacacta tcctttaccc acgctcaaca gtttaataac
     6301 ctgccagcaa taagggatgt tgtttaactt aagtcaaaaa aaatagcgaa ttttccaacg
     6361 acaaaagcta aatatcgcaa aaacctcagt aaaaatcttg ctggagctat tattgctaag
     6421 taacatttac cccctgaagt taatggatca atcaagagag atgtgggctg taatgaatcg
     6481 tcttattgaa ttaacaggtt ggatcgttct tgtcgtttca gtcattcttc ttggcgtggc
     6541 gagtcacatt gacaactatc agccacctga acagagtgct tcggtacaac acaagtaagc
     6601 tctgcacttg tggagcgaca tgctgcccgt ccgggtgcat gttttcactt gtcggatatt
     6661 aaaccaggaa tttattatct tgttcgatgt tgttggtgat tgtcagggat agtaaagcgc
     6721 gttactcttt tcagagcagt ttttcatccc tgaatatttg ccatcagcgc attcttatta
     6781 ttgtaataac acaagcgttc acttttggtg aaattttatc atcactctac ccctttggta
     6841 ttaattattt tatggacctt ttatgactgt tcaggactac ttattaaaat ttcgcaaaat
     6901 cagttcactc gaaagtctgg aaaaactcta cgaccatctt aattacaccc tgacggacga
     6961 tcaggaactg atcaatatgt atcgtgctgc cgatcaccgt cgcgcagagc tggtttctgg
     7021 cgggcgtttg tttgacctcg gccaggtacc gaagtccgtc tggcactatg tccaataaag
     7081 aaagtagcga tcggttataa aagccttata ataacgcccc taaaattaga tttgcctgtt
     7141 tgcgtcgcta ttctggagaa cctatgacca ccacaacgcc acaacgtatt ggaggctggt
     7201 tgcttggccc gttggcttgg cttttagtcg ctctgttgag tacgacgctg gcgttgttgc
     7261 tgtataccgc tgcgttatct tctccgcaaa catttcaaac gctcggcgga caagcactga
     7321 cgacacaaat cttatggggc gtttctttta ttaccgctat cgccatgtgg tattacacac
     7381 tgtggttgac catcgctttc tttaaacgtc ggcgttgtgt tcctaaacac tatattatct
     7441 ggctgcttat ttccgtattg ctggcagtaa aagcattcgc tttttcaccc gtggaagatg
     7501 gcatagccgt tcgccagttg ctgtttactt tgttggcaac cgcattgatc gtcccctatt
     7561 tcaaacgctc gtcgcgggta aaagcgacgt ttgtgaatcc gtaataacct tacagttaac
     7621 ctgttgtcgc ctgctctgga ttaacggata ataggcggct tttttatttc aggccgaaaa
     7681 atgactgact acctgttact gtttgtcgga actgtactgg tcaataactt tgtactggtc
     7741 aagtttctcg gtctctgtcc gtttatgggg gtttccaaaa aactggaaac cgcgatgggc
     7801 atggggctgg caacaacgtt tgtgatgacg ctggcgtcta tttgcgcctg gcttatcgat
     7861 acgtggattt tgatcccact taatctgatt tacctgcgca ccctggcatt tattctggtg
     7921 attgctgtgg tcgtgcagtt caccgagatg gtggtgcgca aaaccagccc ggtgctttac
     7981 cgtctgctgg ggattttttt gccgcttatc accaccaact gtgcagtgct cggcgtggcg
     8041 ttgctgaata tcaatctcgg gcacaatttc ttgcagtcgg cgctgtacgg tttttccgcc
     8101 gctgtcggtt tctcgctggt gatggtgctc ttcgccgcca tccgcgaacg ccttgctgtg
     8161 gctgatgtcc cggcaccttt tcgcggtaat gccattgcgt taattaccgc aggtcttatg
     8221 tctctggcct ttatgggctt taatggtttg gtgaagttgt aatgaatgct atctggattg
     8281 ccgttgccgc cgtgagcctg ctgggcctgg cgtttggcgc cattctgggt tatgcctccc
     8341 gccgttttgc ggtggaagac gatccggtcg ttgagaaaat tgacgaaatc ttaccgcaga
     8401 gccagtgtgg tcagtgcggt tatcccggct gtcgccccta cgcggaagcc atcagctgta
     8461 acggtgaaaa aatcaaccgt tgcgccccag gtggcgaagc tgtgatgcta aaaattgccg
     8521 agttgcttaa tgtcgagccg cagccgctgg atggcgaagc gcaagagcta acgcctgcgc
     8581 ggatggtggc ggttattgat gaaaataact gtattggctg cactaaatgt attcaggctt
     8641 gtccggtaga cgccatcgtt ggcgctaccc gtgccatgca tacggtaatg agtgatctct
     8701 gtacgggctg caatttatgt gttgatccgt gcccgacgca ctgcatctcg ttgcaaccgg
     8761 tcgcagaaac acctgactcc tggaaatggg atctgaacac cattcccgtg cgtatcattc
     8821 ccgtggaaca ccatgcttaa gttattctct gcattcagaa aaaataaaat ctgggatttc
     8881 aacggcggca tccatccacc ggagatgaaa acccagtcca acggtacacc cctgcgccag
     8941 gtaccactgg cgcagcgttt tgttattcca ctgaaacagc atattggcgc tgaaggtgag
     9001 ttgtgcgtta gcgtcggcga taaagtattg cgcggccagc cgcttacccg tggtcgcggc
     9061 aaaatgctgc ctgttcacgc gcccacctcg ggtaccgtta cggctattgc gccccactct
     9121 acggctcatc cttcagcttt agctgaatta agcgtgatta ttgatgccga tggtgaagac
     9181 tgctggatcc cgcgcgacgg ctgggccgat tatcgcactc gcagtcgcga agagttaatc
     9241 gagcgcatac atcagtttgg tgttgccggg ctgggcggtg caggattccc gacaggcgtt
     9301 aaattgcagg gtggcggaga taagattgaa acgttgatta tcaacgcggc tgagtgcgag
     9361 ccgtacatta ccgccgatga ccgtttgatg caggattgcg cggctcaggt cgtagagggt
     9421 attcgcattc ttgcgcatat tctgcagcca cgcgaaattc ttatcggcat tgaagataac
     9481 aaaccgcagg cgatttccat gctgcgcgcg gtgctggcgg actctaacga tatttctctg
     9541 cgggtgattc caaccaaata tccttctggc ggtgctaaac aattaaccta cattctgacc
     9601 gggaagcagg ttccacatgg cgggcgttca tccgatatcg gcgtattaat gcaaaacgtc
     9661 ggcactgctt atgcagtgaa acgtgccgtt attgatggcg agccgattac cgagcgtgtt
     9721 gtaaccctga ctggcgaagc aatcgctcgc ccgggcaacg tctgggcacg gctggggacg
     9781 ccagtgcgtc atttattgaa tgatgccgga ttctgcccct ctgccgatca aatggtgatt
     9841 atgggtggcc cgctaatggg ctttaccttg ccatggctgg atgtcccggt cgtaaagatt
     9901 accaactgtc tgttggctcc ctctgccaat gaacttggcg aaccacagga agaacaaagc
     9961 tgcatccggt gtagcgcctg tgctgacgcc tgccctgcgg atcttttgcc gcaacagttg
    10021 tactggttca gcaaaggtca gcaacacgat aaagctacca cgcataacat tgctgattgc
    10081 attgaatgtg gggcttgcgc gtgggtttgc ccgagcaata ttcccctggt gcaatatttc
    10141 cgtcaggaaa aagctgaaat tgcggctatt cgtcaggaag aaaagcgcgc cgcagaagcc
    10201 aaagcgcgtt tcgaagcgcg ccaggctcgt ctggagcgcg aaaaagcggc tcgccttgaa
    10261 cgacataaga gcgcagccgt tcaacctgca gccaaagata aagatgcgat tgctgccgct
    10321 ctggcgcggg tgaaagagaa acaggcccag gctacacagc ctattgtgat taaagcgggc
    10381 gaacgcccgg ataacagtgc aattattgca gcacgggaag cccgtaaagc gcaagccaga
    10441 gcgaaacagg cagaactgca gcaaactaac gacgcagcaa ccgttgctga tccacgtaaa
    10501 actgccgttg aagcagctat cgcccgcgcc aa
//
LOCUS       DAZPLI010000137        10308 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZPLI010000137 DAZPLI010000000
VERSION     DAZPLI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.138"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(11..2440)
                     /gene="hrpB"
                     /locus_tag="ACHUU3_003334"
     CDS             complement(11..2440)
                     /gene="hrpB"
                     /locus_tag="ACHUU3_003334"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR2083328.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            2514..3044
                     /gene="thpR"
                     /locus_tag="ACHUU3_003335"
     CDS             2514..3044
                     /gene="thpR"
                     /locus_tag="ACHUU3_003335"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR2083329.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            3059..3763
                     /gene="sfsA"
                     /locus_tag="ACHUU3_003336"
     CDS             3059..3763
                     /gene="sfsA"
                     /locus_tag="ACHUU3_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR2083330.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            3941..4396
                     /gene="dksA"
                     /locus_tag="ACHUU3_003337"
     CDS             3941..4396
                     /gene="dksA"
                     /locus_tag="ACHUU3_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR2083331.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            4463..5359
                     /gene="gluQRS"
                     /locus_tag="ACHUU3_003338"
     CDS             4463..5359
                     /gene="gluQRS"
                     /locus_tag="ACHUU3_003338"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR2083332.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            5419..6816
                     /gene="pcnB"
                     /locus_tag="ACHUU3_003339"
     CDS             5419..6816
                     /gene="pcnB"
                     /locus_tag="ACHUU3_003339"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR2083333.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            6813..7292
                     /gene="folK"
                     /locus_tag="ACHUU3_003340"
     CDS             6813..7292
                     /gene="folK"
                     /locus_tag="ACHUU3_003340"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR2083334.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            7662..8246
                     /locus_tag="ACHUU3_003341"
     CDS             7662..8246
                     /locus_tag="ACHUU3_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2083335.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            8351..9091
                     /locus_tag="ACHUU3_003342"
     CDS             8351..9091
                     /locus_tag="ACHUU3_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR2083336.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            9126..10308
                     /gene="htrE"
                     /locus_tag="ACHUU3_003343"
                     /pseudo
     CDS             9126..10308
                     /gene="htrE"
                     /locus_tag="ACHUU3_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT         2573 a         2667 c         2517 g         2551 t
ORIGIN      
        1 aaaaccaacc ttacgaatac tttttcgtcc gtcgcgtcgg tgccgtgttt gccgggtcgt
       61 ccggccagac gtgtttggga taacgccctt tcatctcttt ttgcacctca cggtacgctc
      121 ctttccagaa ggcgctcaaa tctcgtgtga tttgtagtgg cctttgggcg ggtgaaagca
      181 actccagcac cagcggcacg cccccctggg cgatagtcgg attagtggcc tcaccaaaca
      241 tctcctgcat tctcaccgcc agtgcgggcg ggttatcttc atgataacga atggcgatcc
      301 ggcttcccgt cggcacagtg taatgcgcag gcaattcact atccagacgt tgctgcattc
      361 cccaatcaag taatccgcgt agtgcctgat aaatgtccag tgatttcagg ccgcgtagtg
      421 aatgtacgcc tgtcatatgt ggcagcagcc acgtttccag cgctgccaat aaactttcat
      481 catcaaccgc tggccagtca tattccggca accactttgc ggcgcataac aaacgcaagc
      541 gtagctgttc cgcttccgcc gtccagttga gcacgcttaa acctttatca cggatgccat
      601 taagcatcgc ctgatgcaac tcgtcttctg acggtttcgc cagcggctgc actttcaccg
      661 tcaactgacc gatttgtagc cgacgccagg ctttcagcgt accttgcgcg tcatcccatt
      721 ccacggtgtc agattgctgc accagctgcg gacagcgttg tactaattca tcaatatcga
      781 ccggtagcgc cagtaaaatc cgcgcatccg gcgaggcgct gccctgcaat aataacggtg
      841 cgatcaacca ttcgtggcgg cttagcgcgt cgtcggcatc gagcatcgct cccatgccgt
      901 ttgccagttg atagcgtcca tcttgcccac ggcgatgagc aatgcgatcg gcaaacgccc
      961 cggcaagtag cggcgcgata agcgaactgt ctgcctcacc gccgcgtacg tttaagcgtt
     1021 ttaacagttg ctgactacgt tgctgccagg ctggttgatt gcgcgaaaac gccacgccca
     1081 ggtcactatt gcccatcagt ggcggctctt cgagaatggc ggcaattttt gccgcggtag
     1141 cagcttcgtc gtcgttcttc gcgctaacca gcattgccgc taaacgcgga tcgttaccca
     1201 gcgctgccat tttttgccct tgcgcactaa gccgttcacc gtccagcgcc cccagcattt
     1261 gtaacagacg tttcgcggcc attagattca ctactggcgg ttgatccagc cagctcatct
     1321 gcgccggatc gctgcatccc cattgcagta attccatcag caaaccggaa agatcgcttt
     1381 gtaagatctc cggttcactt tgcgcggcag cgcgttctgc ttgttctttg gcgattaaat
     1441 gcaggcagat acccggctcc agacgcccgg cgcgcccggc acgctgcgtc atggatgcct
     1501 ggctaacgcg ttgagtaatc agtcgcgtaa gccccgtgcg cggctcaaaa cgcgccacac
     1561 gctcctgggc acaatccacc accagacgaa taccttcaat ggttaaactg gtttcagcaa
     1621 tattggtcgc cagcaccact ttgcgcatcc cttgcggtgc cgggaggatc gcttttcgct
     1681 gatcgttcag cgacaacgcg ccatacagcg ggcagagcaa cacatcactg ccgatgcgcg
     1741 aagccagttg ttcctgcaca cgctgaattt ctccgacgcc aggtaaaaat aacagtaatg
     1801 atccgctttc ctgacgcagc atttcggcgg tggctaccgc aacggcttcg tcaaaacgct
     1861 gatgcgcggg cagcggtaaa taatggcgtt caaccggaaa cgagcgccct tctgagatga
     1921 cgacgggcgc ttctggcagc atttgctgca agcggtcgtt atccagcgta gccgacataa
     1981 tcagcagttt aaggtcatca cgcagacctt gttgcacatc gagtaacagc gccaacgcca
     2041 aatccgcctg caagctgcgc tcatgaaatt catcaaggat caccaatcca acaccgctca
     2101 actccgggtc acgctggatc atccgcgtca gcacgccttc ggtaactact tccagacggg
     2161 tatttggccc gacgcagttt tgcgcacgca tccggtagcc aacggtatcg cctggctttt
     2221 cgttaagcag ctccgccagc cgttgcgcga cgttacgcgc cgccagacga cgcggctcca
     2281 gcaggataat tttcccgtta atgccgggat gcgccagcag ttgcagcggc agccaggttg
     2341 atttcccggc cccggtcggc gcacttaata atacctgcgg cgcacaatcg agagcggtaa
     2401 gtaattcagg taagacggca gcaacgggca acgacgacac aaaacgctcc agagggttaa
     2461 cattcttcgc gccacattgt agcatcgcgg taattcataa ccgagtgcct cacatgtctg
     2521 aaccgcaacg tctgttcttt gctatcgact tacctgcaga aatccgcgaa cagattatcc
     2581 actggcgcgc cacacacttc ccacctgagg cgggacgtcc ggtcgccgcc gataatttgc
     2641 atctgactct ggcattttta ggcgaagtga gcgccgagaa agagaaggcg ctttctcttt
     2701 tagccggacg gattcgtcaa cctggtttca cactcacgct tgatgacgcc ggacaatggc
     2761 tgcgttcgcg tgtggtgtgg ttagggatgc gtcagccgcc acgcggctta atccagctgg
     2821 cgaatatgct ccgttcacag gctgcccgca gcggttgttt tcaaagcaat cgtccgtttc
     2881 atccacatat taccttattg cgcgacgcca gcgaggcggt gacaatcccg ccgccaggtt
     2941 ttaactggtc ttatgcggtg acggagttca ccctttacgc ctcctcgttt gcccgtggac
     3001 gcacacgcta cacgccgcta aaacgctggg cgctaacgca ataacaagga ttgtcgcaat
     3061 ggaattctct ccccctctac agcgcgcgac gctaattcag cgttacaaac gttttttagc
     3121 cgatgtgatc acacccgatg gtcgcgaatt aacgctacac tgcccgaata cgggtgcgat
     3181 gaccggttgt gcaacgcgtg gcgataccgt ctggtattcg acttcagaca acaccaaacg
     3241 gaaataccca cacacctggg aactaactca aagccagagc ggcgcattta tttgcgtcaa
     3301 tacgctttgg gctaacaggt tgacgaaaga ggctatcctt aatgaatcaa tttcagaact
     3361 gtcaggctat agctcgctga aaagcgaagt aaaatacggc gcagaacgca gccgtattga
     3421 ctttatgttg caggcggatt cgcgtccaga ctgctatatt gaagtgaaat cggttacgtt
     3481 agcggagaac gaacagggat attttcccga tgcggtcact gaacgaggtc agaaacacct
     3541 tcgggagttg atgaacgtag cggctgaagg ccagcgtgcg gttatctttt tcgccgtgct
     3601 gcattcagcc attacacggt tttcacccgc gcgccacatc gatgagaaat acgcgcaact
     3661 attgtcagaa gctcaacaga ggggggtaga aattctggct tacaaagcgg aaatttctgc
     3721 tgaaggcatg gctcttaaaa aatcactgtc ggttacattg tagtaaagta agtaactggt
     3781 taatttacat tctggtcgcg tgcgcaaata cgcttttcct cacacagttg tcaagtgtta
     3841 cgtttagata attgctatcc ggaaaagcat ctgctattta tagcggcctc atttttcccc
     3901 cgaacatggg gatcgatagt gcgtgttaag gagaagcaac atgcaagaag ggcaaaaccg
     3961 taaaacatcg tccctgagta ttctcgccat cgctggggtg gaaccatatc aggagaagcc
     4021 gggcgaagag tatatgaatg aagcccagct ggcgcacttc cgtcgtattc tggaagcatg
     4081 gcgtaatcaa ctcagggatg aagtcgatcg caccgttaca catatgcagg atgaagcagc
     4141 caacttcccg gacccggtag accgtgcagc ccaggaagaa gagttcagcc tcgaactgcg
     4201 taaccgcgat cgcgagcgta agctgatcaa aaagatcgag aagacgctga aaaaagtgga
     4261 agacgaagat ttcggctact gcgaatcctg cggtgttgaa attggtattc gccgtctgga
     4321 agcgcgcccg acagccgatc tgtgcatcga ctgcaaaacg ctggctgaaa ttcgcgaaaa
     4381 acagatggct ggctaattac agccgttcca tcacgtttac cacaggcggg aaatgctccc
     4441 gccttatttt ttgttcaaag agatgacaga cacacagtat attggccgct tcgccccctc
     4501 tccttccggc gagcttcatt ttggctctct gatcgccgcg ctcggcagct atttgcaggc
     4561 tcgcgcccgg caaggtcgct ggctggtacg catagaagat atcgacccgc ctcgtgaagt
     4621 tcccggtgcc gcagaaacta tcctgcgcca gctggaacat tacggtctgc actgggacgg
     4681 cgatgttctc tggcaatcgc aacgtcacga cgcctatcgt gaagcactcg cctggttaca
     4741 tgaacaagga ctaagttatt actgcacctg tacgcgtgcg cgtattcaaa gcattggcgg
     4801 tatttacgac ggtcattgcc gggtgttgca tcatggacca gacaatgccg cagtgcgtat
     4861 ccgccagcag catccggtca cgcaatttac tgaccagctg cgcggcatta ttcacgccga
     4921 cgaaaaactg gcacgggaag attttatcat tcatcgccgt gatgggttgt tcgcctacaa
     4981 cctggctgtt gtggttgatg atcatttcca gggcgttaca gaaatagtgc gtggggctga
     5041 tctgattgaa ccaacagtaa ggcaaatctc gctgtaccag ctttttggct ggaaagtgcc
     5101 agattacatt catctgccgc tggcgcttaa tccacaaggc gctaaacttt ccaagcagaa
     5161 tcatgcgcct gcgttgccga aaggcgatcc acgtccggta ctaatcgcgg cacttcaatt
     5221 tctgggtcag caggcagaag cacactggca ggatttcagc gtcgaacaaa tccttcagtc
     5281 agcagtcaaa aactggacgc tgaccgccgt gcccgagtcg gcaattgtaa attcaacatt
     5341 ctcaaatgcg tcatgctgag ctatgattag ccgctatttt tttgtcctga atgatgtttg
     5401 acactaccga ggtgtactat ttttacccga gtcgctaatt tttgccgcaa ggtgctaagc
     5461 cgcgaggaaa gcgaggctga acaggcagtc gcccgtccac aggtgacggt gatcccgcgt
     5521 gagcagcatg ctatttcccg caaagatatc agtgaaaatg ccctgaaggt aatgtacagg
     5581 ctcaacaaag cgggatacga agcctggctg gttggcggcg gcgtgcgcga cctgttactt
     5641 ggcaaaaagc cgaaagattt tgacgtaacc actaacgcca cgcctgagca ggtgcgcaaa
     5701 ctgttccgta actgccgcct ggtgggtcgc cgtttccgtc tggctcatgt aatgtttggc
     5761 ccggagatta tcgaagttgc gaccttccgt ggacaccacg aaggtaacgt cagcgaccgc
     5821 acgacctccc aacgcgggca aaacggcatg ttgctgcgcg acaacatttt cggctccatc
     5881 gaagaagacg cccagcgccg cgatttcact atcaacagcc tctattacag cgtagcggat
     5941 tttaccgtcc gtgattacgt tggcggtatg aaggatctga aggacggcgt tatccgtctg
     6001 attggtaacc cggaaacgcg ctaccgtgaa gatccggtac ggatgctgcg cgcggtacgt
     6061 tttgccgcca aattgggtat gcgcatcagc ccggaaaccg cagaaccgat ccctcgcctc
     6121 gctaccctgc tgaacgatat cccaccggca cgcctgtttg aagaatcgct taaactgcta
     6181 caagcgggct acggttacga aacctataag ctgttgtgtg aatatcatct gttccagccg
     6241 ctgttcccga ccattacccg ctacttcacg gaaaatggcg acagcccgat ggagcggatc
     6301 attgaacagg tgctgaagaa taccgatacg cgtatccata acgatatgcg cgtgaacccg
     6361 gcgttcttgt ttgccgccat gttctggtac ccactgctgg agacggcaca gaagatcgcc
     6421 caggaaagcg gcctgaccta tcacgacgct ttcgcgctgg cgatgaacga cgtgctggac
     6481 gaagcctgcc gttcactggc aatcccgaaa cgcctgacga cgttaacccg cgatatctgg
     6541 cagttgcagt tgcgtatgtc ccgacgtcag ggtaaacgcg catggaaact gctggagcat
     6601 cctaagttcc gtgcggctta tgacctgttg gccttgcgag ctgaagttga acgtaacgct
     6661 gaactgcagc gtctggtgaa atggtggggt gagttccagg tttccgcgcc accagatcaa
     6721 aaagggatgc ttaacgagtt ggatgaagag ccgtcaccgc gtcgccgtac tcgtcgtcca
     6781 cgcaaacgcg caccgcgtcg tgagggtacc gcatgacagt ggcgtatatt gccataggca
     6841 gcaatctggc ctctccgctg gagcaggtca atgctgccct gaaagcatta ggcgatatcc
     6901 ctgaaagcca cattcttgcc gtttcttcgt tttaccgcac tccaccgctg gggccgcaag
     6961 atcaacccga ttacctcaac gccgccgtgg cgcttgaaac ttcgctcgcc ccggaagagt
     7021 tactcaacca tacacagcgc atagaactgc aacagggtcg tgttcgtaaa gctgaacgct
     7081 ggggaccacg cacgctggat ctcgatatca tgctgtttgg taatgaagtg ataaataccg
     7141 aacgcctgac cgttccgcac tacgatatga agaatcgtgg atttatgctg tggccgctgt
     7201 ttgagattgc accggagctg gcgtttcctg atggggagac gttgcgtgaa gtcttacata
     7261 caagagcatt tgacgaatta agcaaatggt aatttattta tattacgaat ataaatattt
     7321 tctgaaaatc atacatcctc tctctcctta ataaaaattt tagtttaaac atttttgcag
     7381 aaacataata taaattcccg cagataaata tcaatcccat cgccataccc taaccaattg
     7441 cacttgtgaa tttatttttg cttaattttg aattaagaaa atttaatttg ttcaaaagcc
     7501 cttaaagtgc acaataggcg tcctaatgaa caatggttgt aaattttggt aacaaatatc
     7561 attgctcatt aatatctagc atctgcttat aacatttgtg tcatgaatag tcattcttca
     7621 aaccagtagt ctgactaatg gaattttatt caaggatttt tatgtctaaa aaattaggtt
     7681 ttgccctgtc tggtcttatg ctggcaatgg ttgcaggtac tgcttctgct gatatggatg
     7741 gtggtcagtt aaatatcagt ggtctggttg ttgataacac ctgtgaaacc cgcgttgacg
     7801 gcggtaataa agatggtctg atcctgctgc aaactgcaac tgttggtgaa atcgatgctg
     7861 gcgtgctgaa tgacaccgtt ggcgctaaag ccaaaccttt cagcatcacc gttgactgca
     7921 gcaaagcaaa tccgaatcca ggtagtacag ctaaaatgac cttcggttct gttttctttg
     7981 gtaatagcaa aggaacgctg aacaacgaca tgtctattaa caacccgtct gacggcgtga
     8041 atatcgctct gcacaatatt gatggttcca ctatcaaaca ggttcaaatt aacaaccctg
     8101 gcgatgtgta tactaaagcc ctggatgcaa cgacaaaatc tgctgtttat gattttaaag
     8161 cgtcttacgt tcgtgccgtc gcagaccaaa cagcaactgc tggttatgta aaaactaaca
     8221 ctgcatacac cattacttat cagtaagtat tcaccaatta aattgattga atgaatatac
     8281 agggaatagt gttaataatt tctattttta tattattccc tgttttaatt aactctgtca
     8341 gggatggttt atgtttttta acaccaaaca tacaacagct ttatgcttcg taacctgtat
     8401 ggcttttagt tcatcgtcta ttgcggacat tgtcatttcg ggtactcgcg taatatataa
     8461 aagcgatcaa aaaagtgtca acgtacgtct ggaaaataaa gggaataacc cgttgcttgt
     8521 ccagagttgg ttagatactg gcgataacaa cgctgagcct ggcagtatta cagtcccttt
     8581 tactgccacg ccgccagtat cgcgtattga tgccaaacgt gggcaaacaa tcaaattaat
     8641 gtacacagcc agcacctcac tgcctaaaga cagagagagc gtgttctggt ttaacgtact
     8701 ggaagttcca ccaaaaccag atgcagaaaa ggtcgcgaat caaagtctgc tgcaactggc
     8761 atttcgcaca cgtataaaac ttttctatcg cccggatgga ttgaagggaa atccctctga
     8821 agccccgtta gccctgaagt ggttctggtc aggttcagaa ggtaaggcgt cattgcgagt
     8881 gaccaatcca accccttact acgtctcttt tagcagtggt gatttagaag ctagcggtaa
     8941 acgctatccg attgatgtga aaatgattgc accatttagt gatgaggtca tgaaagtcac
     9001 tggccttaat ggcaaagcaa gttctgcaaa agtacatttt tacgccatta atgattttgg
     9061 tggcgccatt gaaggtaatg ccagtctgta atcaggcaag gatataattc cgcaggaagc
     9121 ataacgtgac tataaaacca actaaaaatt atcataatca tctgacccgt atcgctacgt
     9181 tttgcgcgct gctgtattgc aatagtgctt tctgtgctga acttgttgaa tatgaccata
     9241 ccttcctgat ggggcagaat gcctcaaata ttgatctcag ccggtatagt gaaggtaacc
     9301 ctgctatacc gggtatgtac gacgtcagtg tttatgtcaa cgatcaacct attattaacc
     9361 aaagcattac atttattgaa attgaaggaa aaaaaacgca caagcctgta tcacacttaa
     9421 gaatttattg cagtttcata taaattctcc cgatataaat aacgaaaaag ccgttctgct
     9481 tgccagggat gaaacactcg gcaattgcct taatttgacg gaaattatcc ctcaggcttc
     9541 tgttcgttat gacgttaacg agcaacgtct ggatatagac gttcctcagg catgggtaat
     9601 gaaaaattac caaaactatg ttgatccatc gttatgggaa aatggcatta atgcggccat
     9661 gttgtcatac aacctcaacg gatatcatag tgaaactcct ggtcgaagaa atgacagcat
     9721 ttatgctgct tttaacggtg ggatgaattt aggtgcatgg cgactgcgtg cctcaggcaa
     9781 ctacaactgg atgaccgatt ctggcagtaa ttatgatttt aagaatcggt atatacagcg
     9841 tgatatcgcc tcgctgcgtt ctcaactcat tcttggtgag tcttatacaa cgggcgaaac
     9901 ctttgattct gtcagtatcc gcggcattcg tttatacagc gacagccgca tgttgcctcc
     9961 gacattagcg agttttgcgc ctatcattca tggcgttgct aataccaacg ccaaagtcac
    10021 tattacgcaa ggtggctata agatttatga aacgactgtg ccgccaggcg ctttcgtcat
    10081 tgatgatctg agtccgtcag gttacggcag cgatcttatt gttactgtcg aagaatccga
    10141 tggctcaaag cggacattct cgcaaccttt ttcatcggtc gttcaaatgt tacgccctgg
    10201 cgttggacgt tgggatatta gcggcggcca ggtcttaaaa gatgatattc aggatgagcc
    10261 taatttattt caggccagct actactacgg tctgaataac tatctaac
//
LOCUS       DAZPLI010000138        10268 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZPLI010000138 DAZPLI010000000
VERSION     DAZPLI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10268
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.139"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="ACHUU3_003344"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="ACHUU3_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR2083337.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHUU3_003345"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHUU3_003345"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR2083338.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHUU3_003346"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHUU3_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFR2083339.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHUU3_003347"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHUU3_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR2083340.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHUU3_003348"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHUU3_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHUU3_003349"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHUU3_003349"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR2083341.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHUU3_003350"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHUU3_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR2083342.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHUU3_003351"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHUU3_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR2083343.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="ACHUU3_003352"
     CDS             complement(9457..10149)
                     /locus_tag="ACHUU3_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR2083344.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2499 a         2474 c         2720 g         2575 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaa
//
LOCUS       DAZPLI010000139        10158 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZPLI010000139 DAZPLI010000000
VERSION     DAZPLI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10158
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.140"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..1031
                     /locus_tag="ACHUU3_003353"
     CDS             <1..1031
                     /locus_tag="ACHUU3_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HFR2083345.1"
                     /translation="TIRTHAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCA
                     GAIASPQILQRSGVGNAELLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYP
                     ALQWWNQPKIGAEWLFGGTGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGS
                     NAVKEHGFQCHVGSMRSPSRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITR
                     EIMHQPTLDQYRGREISPGTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDG
                     EGRVHGLEGLRVVDASIMPQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVAN
                     GMPVRAKK"
     gene            1244..1912
                     /gene="ykgH"
                     /locus_tag="ACHUU3_003354"
     CDS             1244..1912
                     /gene="ykgH"
                     /locus_tag="ACHUU3_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFR2083346.1"
                     /translation="MSEQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYVNK
                     DNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQRITTFFHTSPNVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQIPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(2155..2850)
                     /locus_tag="ACHUU3_003355"
     CDS             complement(2155..2850)
                     /locus_tag="ACHUU3_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR2083347.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(2843..4270)
                     /locus_tag="ACHUU3_003356"
     CDS             complement(2843..4270)
                     /locus_tag="ACHUU3_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR2083348.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(4281..5000)
                     /locus_tag="ACHUU3_003357"
     CDS             complement(4281..5000)
                     /locus_tag="ACHUU3_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR2083349.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(5528..6382)
                     /gene="rclR"
                     /locus_tag="ACHUU3_003358"
     CDS             complement(5528..6382)
                     /gene="rclR"
                     /locus_tag="ACHUU3_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFR2083350.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            6608..7933
                     /gene="rclA"
                     /locus_tag="ACHUU3_003359"
     CDS             6608..7933
                     /gene="rclA"
                     /locus_tag="ACHUU3_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFR2083351.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            8042..8278
                     /gene="rclB"
                     /locus_tag="ACHUU3_003360"
     CDS             8042..8278
                     /gene="rclB"
                     /locus_tag="ACHUU3_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFR2083352.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            8290..8883
                     /gene="rclC"
                     /locus_tag="ACHUU3_003361"
     CDS             8290..8883
                     /gene="rclC"
                     /locus_tag="ACHUU3_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFR2083353.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(9043..9912)
                     /locus_tag="ACHUU3_003362"
     CDS             complement(9043..9912)
                     /locus_tag="ACHUU3_003362"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR2083354.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         2772 a         2436 c         2244 g         2706 t
ORIGIN      
        1 tgaccattcg tactcacgct atgaccgatc acatcatttt tgacggcaaa cgcgcggtgg
       61 gcgtcgaatg gctggaaggc gacagcacca tcccaacccg cgcaacggcc aacaaagaag
      121 tgctgttatg tgcaggcgcg attgcctcac cgcagatcct gcaacgctcc ggcgtcggca
      181 acgctgaact gctggcggag tttgatattc cgctggtgca tgaattaccc ggcgtcggcg
      241 aaaatcttca ggatcatctg gagatgtatc tgcaatatga gtgcaaagaa ccggtttccc
      301 tctaccctgc cctgcagtgg tggaaccagc cgaaaatcgg tgcggagtgg ctgtttggcg
      361 gcactggcgt tggtgccagc aaccactttg aagcaggcgg atttattcgc agccgagagg
      421 aatttgcgtg gccgaatatt cagtaccatt tcctgccagt agcgattaac tataacggct
      481 cgaatgcggt gaaggagcac ggtttccagt gccacgtcgg ctcaatgcgc tcgccaagcc
      541 gtgggcatgt gcggattaaa tcccgcgacc cgcaccagca tccggcaatt ctgtttaact
      601 acatgtcaca cgagcaggac tggcaggagt tccgcgacgc aattcgcatc acccgggaga
      661 tcatgcatca accgacgctg gatcagtatc gtggccgcga aatcagcccc ggcacggaat
      721 gtcagacgga tgagcagctc gatgagttcg tgcgtaatca cgccgaaacc gccttccatc
      781 cgtgcggtac ctgcaaaatg ggttacgacg agatgtccgt ggttgacggc gaaggccgcg
      841 tgcatgggct ggaaggcctg cgcgtggtgg atgcgtcaat tatgccgcag attatcaccg
      901 ggaatttgaa cgccacgaca attatgattg gcgagaaaat agcggatatg attcgtggac
      961 aggaagcgct gccgaggagc acggcgggat attttgtggc aaatgggatg ccggtgagag
     1021 cgaaaaaatg agtcgtgatg tgaactaacg caggaaccga tcagattgag agttaccgtt
     1081 ccagagaggg ggaccgaatc cttatataaa cactgaggta actctcatgc ttcatatcca
     1141 gtattcatga tgctggtttt ttgctgatat ttaccccgaa cattcctgat acagactatt
     1201 aatacaacct tatctgcggc agattttatt ttctggatat attatgagtg aacaaatcaa
     1261 acaggatatc gatctgattg agattttatt ttatctgaag aaaaagattc gtgttatcct
     1321 ttttattata gctatctgta tggcaatggt gcttttattt ctgtatgtca ataaagataa
     1381 tataaaagtg agttacagcc taaaaataaa ccagacaaca ccaggcatac ttgttaactg
     1441 tgatagcaat aataactttg cctgtcagac cacaatgact gaagatgtta tacagcgaat
     1501 tactacattt tttcacacca gcccaaatgt caagaacaga gaaataaagc tggaatggtc
     1561 aggagataag agagatttac caactgctga agcggaaata tctcgcgtgc aggcgtctat
     1621 tatcaaatgg tatgcgtcag aataccataa tggcaggcaa gttctcgatg agatacaaat
     1681 accttcagca attaacagtg agctttatac aaaaatgata tacctgacca ggaactggtc
     1741 attgtatccg aacggtgatg gctgtgtaac tataagctca ccagaaataa aaaataaata
     1801 ccctgctgcc atttgcctgg ctctgggatt ttttctaagc attgtaattt ctgtaatgtt
     1861 ttgccttgtc aaaaaaatgg tagatgaata ccaacaaaac tctgggcagt aataaacagc
     1921 attctgatag agagtgcctg tataaagctg aagtgacaat gaccgggaag ggaacccgtt
     1981 ttcaggaatg gatgcctgtc tttttcactg taaatatcct ggcattacta atgccaggat
     2041 atttacagaa gagcagcata accatgacgg aacggggtta agtcaggttg tccttaacgc
     2101 tattttcaac ctcgtatgcc ttcttcaggt ttatgttcag atctcatatc tctctcagca
     2161 atcctcaata atcagataca ccgctttcac cggaccatga accccgacga ctttgataag
     2221 ctcaatatcc gccgttgagc tgggaccact gatgatgtta atacatgacg gcattcgttc
     2281 accggcctgc gctttctgat gcaatttttc tgcgagttgc gctacacgcg gcaggattgt
     2341 gcttttacgc aggataaaaa gagaagattc cgggagcagg ctcaatgaac gcccgcgctc
     2401 gacggcggaa aaaagaacca cgcctcccga ttcggttaaa ccatattcag catacacgac
     2461 acccacttta gcctgctctg cctgcgagat attctcggca cctttcgccg gatcccaaac
     2521 aacggcattg cattcctgct gcaaacgttc actaatcccc aattcctcca gcctcgtgtc
     2581 accgctaatc acgaccgact gatctcccag ctctttacac agacgtattg cagcttctgc
     2641 cgccttcgcc tcgctggtca gctcacagcg cgtcaacata acatcgctgg caaactgaat
     2701 aaacgcgtca cagcgctgct gttggttaag ttgggtaagc cgctcgttag catagttgtt
     2761 aagcggcgca tcttctgctt gcggttcaag tcgcagcggg cgacccagtg cctgagcaac
     2821 gttattcaaa aattcgctcc gattatccat tctttttctc ctgcgcctga tgtttcttaa
     2881 accaactacg gaaactctct ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc
     2941 taatcgcgcc aaatttgagt ggtgttttgc cgccattgat aaaccagctt gccgcatgag
     3001 cgccggccat catcccgact ttccacaatc ctggatgact attggcataa gcgaacattt
     3061 ttatcgcccg ttgctctgct tttgcggtga tccctttttc agccatcacc cgacgatgac
     3121 gcaaaatcag ttttgacagc ggaatacgca ccggacacac gctgtcacaa gcagtgcata
     3181 aagagcaggc gtagggtaaa tctttaaaat ctttatagcc gccaagtagc ggagaaatca
     3241 ccgcaccaat tggccctgga taaatagagc catatccatg accgccaata tggcgatatg
     3301 ccggacaagt attcatacaa gccccgcagc gaatacagcg cagcacatcc cgaaattcag
     3361 aggccagcac ctcagaacgc ccgttatcga caataaccag atgaaactct tcaggaccat
     3421 caacgtgccc cgcttcgcgc ggtcctgtca gccaggtgtt gtatcccgtc aaacgtgcac
     3481 caacggcact gcgcgccagc atggtgatca atacatctac ctcggcaaac gtgggggcaa
     3541 tacgctccat tcccatcact gcaatatgcg ttttaggcag cgtggtacac attcgcgcat
     3601 taccttcatt ggtcactagg cataccgaac ctgtctctgc caccgcgaaa ttacagccgg
     3661 taatacctat ttcagcactg aggaaatctt cgcggatttt ttgccggatg aataaggtca
     3721 tcgcttcagg cgtttccggc ccctcatagc ccagacgttc gtgtagcact cgacggatct
     3781 gatggcgatc tttatgaatt gccgggacca caacatgaga tggcggatct tgatccagct
     3841 ggagaatata ttcacccaga tcggtttcaa tcacctgaat gccagcatcc tgcaacacat
     3901 gattgacacc aatctcttcg gtcaccatcg atttagattt caccaccttc cgggcatttt
     3961 tgcgttgggc aacctgtaaa atgtagcggg tagcgtcttc tttggttttt gcaaaccaga
     4021 cgtgaccgcc gttttgcgtc actttttctg agagctggta cagataagcg tcgagattac
     4081 tcagaacatg atcacgtatc tgggcggccc gatcgcgcca ctcctcccag tgccccaatt
     4141 catcgaccat tttttgccga tttgccccaa tacgctgctg cgcgtttgcc accgctttgc
     4201 gcatgatcgg atcttcaatt tgctgacgga tgcgtgtctt aaaatctgta ttactggttt
     4261 tgatcgacat ctttatatcc tcagcggctc atcaacactt cagcaatatg catcactttg
     4321 actttctgcc cttcccgttg taatcgccca ctgatgttca gcaggcaact cacgtcagca
     4381 ccaattaaat actcagggcg gacttccatc aggtgcacaa ccttttcttt caccatctcg
     4441 ccggatattt cggccatttt gaccgagaac gtgccgccaa atccgcagca ggtatcctgt
     4501 tcagcaaagg tcaacagctc cagtccacgc acatttttca gcagcgtaag tggctcgtcc
     4561 ttcactccca gcttacgggc caggctacaa gatgggtgat acacagctct cccttgcaaa
     4621 ctggcaccta catcgactac ccctaattta ttaacaataa aagaggtgag atcctgcata
     4681 cgcgcggcaa ccttttcggc acgtgatgcc cattcgggtt catccgccag atacgtcggg
     4741 taacttttta cggcataggt gcaagagcca gccggtgaaa taatgggatc gtcgttatcc
     4801 tccagtgcgg cgatcagatt tttcatccct ggaatcgctt ctttgatata accgctattg
     4861 atcgcaggct gaccgcagca tccctgtttc tccgggaaat ttacgcgaca gccgagtttt
     4921 tccagtagca gcacggagtc tcgtgccatt cttgatttca gggcgtcacc aatacaggtg
     4981 acaaagaaat tgacattcac aactaatact ccattacttc atgcccattt atgcccgatg
     5041 gttcccctcc ttccggggga tcaatcatca ataaatatgg cgttcttttt taattggcta
     5101 gcaacatacc acccctcatt tgtgtgacaa atgataatct ttcatccaaa cttcaaatgc
     5161 aataactaat tttatttctt tcggcatcgc tcacatttta tggggaaatc gacgttcatt
     5221 tttggcataa gaaatccatt taaaaaggct acatccctta tacctattca gagctactta
     5281 ttcaggggtt attgagatat acactgatgg tgtacaaaaa aatcaccgac aataatcacg
     5341 tatcaagtaa tagtcggggt tttatttaat tgaagcaatt tagtttgcat ttttatttaa
     5401 aaactgagct agagtccatc agaaaaaaat atataaagcc caaaccaccg ttggaaatag
     5461 aaatagttga aaggctcaca actcccggaa tgcggattat ctttgccctg atttctggcg
     5521 gttttattca gggtgcaagc tgtctgaccc tttcccgata ttctcccgga gtacaaccaa
     5581 actcgcggac aaacgccttg tgaaaagatg attcactggc atagcctact gactcagcga
     5641 tcaccacaac agggagcatt tcccgggaaa acatctgggc cgctatttgt agacgcaact
     5701 ttgttaatac agccagcggc gtggttccgg aaacatcacg gaaaagctgg gcaaaacttg
     5761 cccgagacat gtgggcgatg ctggccagcg attcgacggt ccaggcgtgt cctggcattt
     5821 ccagcatttg ctgtattacc gcaccaagac gtggatgcag aagcaaactg agaatgtttt
     5881 tctctgtatt aacctgtgca atccactcac gcaccgcgag ggtaaagaat gtagcgcaga
     5941 tctggctaca cagtgcatcc actcccggca tcgccgatct ggattcctgt tgtaaaaacg
     6001 ggatcgcctc cctcagccag ttatattcca cgctgtggtt aaccggtgct aaaaacagcg
     6061 tttccggcaa agacgttaaa aaataacgcg ccgaatgttg caaccgaaga gtgccgcaga
     6121 caatacaggt cgattcatta tcgacatgac tcaggcgatg agctgaattt tgtggtagca
     6181 ggaccacatt tcccgggcgt aatgtaaaaa tctctcccgt cggcatttcc agcttcgccg
     6241 ctccttgcgt taacgcatgc caacgaataa ccgataattc cccggcacca tgttgaagct
     6301 gccagtcact tcctaacacg caattcttat cgatcgttcc ttgtggagcg ttaagcatca
     6361 acaaacggct aagggcatcc atatcagact cctgagtggt ttttgattac aaacaccaac
     6421 aatcttagta aataactaat ctaataccat caaatggtta atcatgattt ttgttttgtg
     6481 attctgatta agaaaaagaa aataccagac gataagacaa aatatcaagc gttgtgaaga
     6541 aatggtattt gctcttttgc gtctatagtc atgatgtcaa atgaacgtgt ttcgacagga
     6601 aatcatcatg aataaatatc aggcagttat tattggtttt ggcaaggctg gaaaaacatt
     6661 agccgtcacg ctggcaaaag caggttggcg agtggctctc atcgaacaat caaatgcaat
     6721 gtatggcggg acctgtatta atatcggctg catcccaacc aaaacattgg ttcatgacgc
     6781 acagcagcac acagattttg tccgtgccat acagcgtaaa aatgaagtgg ttaatttttt
     6841 acgtaataag aattttcata atcttgcgga tatgcccaat atcgacgtga tcgacggcca
     6901 ggcggagttt atcaataatc atagcctgcg tgttcatcgg cctgagggaa atctggaaat
     6961 tcatggcgag aaaattttta ttaataccgg tgcacaaacc gtggttccgc caattcctgg
     7021 aattaccacc acgcctggtg tatatgacag caccggatta cttaatctaa aagaattgcc
     7081 tgggcattta ggtattttgg gcggcggata tattggcgtt gagttcgcct ctatgttcgc
     7141 taattttggc agcaaggtaa ccattttaga agcagcttcg ctgtttttgc ctcgggaaga
     7201 tcgggatatt gctgataata tcgcgacgat tttacgcgat cagggcgtcg atattatcct
     7261 caatgcccat gtggagcgaa tcagtcacca tgaaaatcaa gtgcaagtgc atagcgagca
     7321 cgcccaactg gcggtggatg cactgttaat agcttccggt cgtcaaccgg ctaccgcttc
     7381 gttacatcca gaaaatgccg gtatcgcagt aaacgagcgc ggggcaattg tcgttgacaa
     7441 gcaattacat accaccgcag acaatatttg ggcgatggga gatgttaccg gcgggctgca
     7501 atttacttat atttcactgg atgattaccg cattgtgcgt gatgagttgc tgggagaagg
     7561 cagacgtagt actgatgatc ggaaaaatgt gccttattcc gtattcatga caccgccact
     7621 gtccagggtt ggtatgacag aagaacaggc cagagagagt ggtgctgata ttcaggtggt
     7681 gacattgcct gtagctgcaa ttccgcgtgc cagagtgatg aatgatactc gtggggtatt
     7741 aaaagcgatt gttgataata aaacccaacg tatattaggg gcatcactgc tgtgtgttga
     7801 ctcccacgag atgatcaata tagtgaaaat ggtgatggat gccgggctgc cttatagcat
     7861 attacgcgat cagatattta ctcatccgtc gatgagcgaa tcactcaatg atctattttc
     7921 attagtcaaa taaactcaaa atcagacgcc agaacaaata ttctggcgtc tcagagaaaa
     7981 gaatcttatt aattctctgt tactctataa cttcttaatc acttcattga tggtatttta
     8041 tatgtttaaa aaatctgttt tatttgcaac actattatct ggcgttatgg aattttccac
     8101 caatgcagat gataaaataa ttctgaaaca tatcagcgtc tcgtcagtat cagcatcacc
     8161 gacagttctg gaggatacca ttgctgatat agccagaaaa tataatgctt catcctggaa
     8221 agtcacatcg atgcgaattg ataataattc aaccgcaaca gcagtattgt ataaataagg
     8281 atgttcacaa tggaaaaata cctgcacctg ttaagtcggg gagataaaat tggcctgtca
     8341 ttgattcgtc tgagtattgc gattgttttt atgtggattg ggttattaaa gtttgtccct
     8401 tacgaggcag acagcattac accattcgtc gcaaacagtc cactaatgtc gttcttttat
     8461 gaacacccgg aagactataa acagtatctg actcacgaag gcgaatacaa accagaagca
     8521 agggcatggc aatcggccaa taatacctat ggtttttcca acggtcttgg cgtcgtggag
     8581 gtgattattg ctctgctggt tttggctaat cctgtcaatc gctggttagg tttattggga
     8641 ggactgatgg catataccac gccgctggta acactctcat ttttaatcac caccccggag
     8701 gcatgggtac ccgcattggg tgacgctcat catggtttcc cttatttatc cggtgctggt
     8761 cgcttggtat tgaaagatac tctgatgctg gcaggtgctg tcatgataat ggcagattcg
     8821 gcgcgggaaa ttcttaaaca acgcagtaat gaatccagtt caacgttaaa aactgaatat
     8881 tgataatccg aacactgtct gttgttcact ttttcaggcg tcaggccccc tttctgatga
     8941 tgaagcctga cgccatatta ccggagatat gacagagtga agatcaatat cactcccctg
     9001 ttaaaagatt cttgtcgtct caggtaaaat atatgattaa tatcaatcat gtatttttac
     9061 agcaagacaa ccacgaacaa attcaggatt aaaatgtttt accgactccc caacataacc
     9121 caaatcaagc gaactaattt gtgccatttc tttctctgtc agtgagaaat cccagatagc
     9181 aaaattttct tcgatacgct gttgatgtgt cgatttcgga ataacgacca ctccccgttg
     9241 aatattccaa cgcagaatga cttgagaaat cgatttttga tgggcatctg caatactctg
     9301 aagcagatta ttttcaaagg gtttatgtcg tccaccacct aacggagccc atgcttcagg
     9361 ctgcacgtta taatacttca tggttgccag cgcctctggt tgggcaaaat agggatgcaa
     9421 ctcgacctgg ttgaccatcg gtttaactct taccgtttca caaaagttcg ccagaacatg
     9481 aggatagaaa ttggaaaccc caattgcttt taatttgccc tcttcatagg catcttcgag
     9541 tgcacgccac acactgaaat aatcgcgcat agcctggtgc aataaataga ggtcgaagta
     9601 ctctagtccc gattttttta atgatgcttc aatacctgct gctgccgtat cctgattcaa
     9661 catatcctgc ccccacaatt tagacgttat aaataactca tctcgggtac ataaaccttc
     9721 agatatagct tcacgaacgg cttcaccgac ggcatcttca ttaccgtata ccgctgcagt
     9781 gtcgatgagt cgataacccg tgcggatagc gttcaacact gactgtttgc acacgttttt
     9841 atcggtaacc tgaaaaacac caaatcccat catcggcatt ttcagattgt tacttaatac
     9901 ggaaaattcc actgtcagtc cctccattag gcatgaacaa tgagtctacg ttaaaacata
     9961 acctcaaatt agtatgtgga ttttgatatc acttatgcaa aaaattcatt aataatgtag
    10021 gactgaaacc tctctatttt cggggacaac gaagcagacg ctaccagtgc ttttgccttc
    10081 gaccttgctg tttttgatac acttagggcc ctgggcataa cgaaaatgtg cgatatcacg
    10141 aagaattaat ggagaatg
//
LOCUS       DAZPLI010000140        10016 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZPLI010000140 DAZPLI010000000
VERSION     DAZPLI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10016
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.141"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(268..343)
                     /locus_tag="ACHUU3_003363"
     tRNA            complement(268..343)
                     /locus_tag="ACHUU3_003363"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(308..310),aa:Asn,seq:gtt)
     gene            complement(444..1241)
                     /gene="mtfA"
                     /locus_tag="ACHUU3_003364"
     CDS             complement(444..1241)
                     /gene="mtfA"
                     /locus_tag="ACHUU3_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR2083355.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            1335..1424
                     /locus_tag="ACHUU3_003365"
     tRNA            1335..1424
                     /locus_tag="ACHUU3_003365"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1369..1371,aa:Ser,seq:cga)
     gene            complement(1994..2524)
                     /locus_tag="ACHUU3_003366"
                     /pseudo
     CDS             complement(1994..2524)
                     /locus_tag="ACHUU3_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(2867..3517)
                     /gene="zinT"
                     /locus_tag="ACHUU3_003367"
     CDS             complement(2867..3517)
                     /gene="zinT"
                     /locus_tag="ACHUU3_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR2083356.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(3774..4409)
                     /gene="msrQ"
                     /locus_tag="ACHUU3_003368"
     CDS             complement(3774..4409)
                     /gene="msrQ"
                     /locus_tag="ACHUU3_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR2083357.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            complement(4410..5414)
                     /gene="msrP"
                     /locus_tag="ACHUU3_003369"
     CDS             complement(4410..5414)
                     /gene="msrP"
                     /locus_tag="ACHUU3_003369"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFR2083358.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(5523..5936)
                     /gene="hiuH"
                     /locus_tag="ACHUU3_003370"
     CDS             complement(5523..5936)
                     /gene="hiuH"
                     /locus_tag="ACHUU3_003370"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR2083359.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            6069..6740
                     /gene="hprR"
                     /locus_tag="ACHUU3_003371"
     CDS             6069..6740
                     /gene="hprR"
                     /locus_tag="ACHUU3_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR2083360.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            6740..8098
                     /gene="hprS"
                     /locus_tag="ACHUU3_003372"
     CDS             6740..8098
                     /gene="hprS"
                     /locus_tag="ACHUU3_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR2083361.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(8206..9057)
                     /gene="hchA"
                     /locus_tag="ACHUU3_003373"
     CDS             complement(8206..9057)
                     /gene="hchA"
                     /locus_tag="ACHUU3_003373"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR2083362.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            9204..9614
                     /locus_tag="ACHUU3_003374"
     CDS             9204..9614
                     /locus_tag="ACHUU3_003374"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083363.1"
                     /translation="MLNIIHAKFSLLSNVVASGLNDFRPLSKVRGGGERFSAVKYCGT
                     DEYLYRGLVEKRLFHALFLQIFLYGKRVCYGLIHKEMHLGVGLKSRWLMLLCQEGQGK
                     VLYRYALAIACAADGDEMPIKKPEFDEGRTQASV"
     gene            complement(9648..>10016)
                     /locus_tag="ACHUU3_003375"
     CDS             complement(9648..>10016)
                     /locus_tag="ACHUU3_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR2083364.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
BASE COUNT         2825 a         2066 c         2297 g         2828 t
ORIGIN      
        1 cagcgaaaaa atgtaaatac tgatagcaat acgattgaaa attaataact tttcgttagg
       61 cagttttggg tgtgagttgc aagaggggag actactgaat aactcaagtt ttataatcga
      121 ggggaaaatg gtgatggcgt tcatagcaaa acgccctcaa ccataaaggt cgagggcgct
      181 taaggtgtta aaaacccgct atccgttaaa aaacaatgtt caactaaggt cagtgacatt
      241 gcgctaaaaa agcgaatttt tagaatttgg ctcctctgac tggactcgaa ccagtgacat
      301 acggattaac agtccgccgt tctaccgact gaactacaga ggaatcgtgt gaacggggca
      361 catattactt agcggtacct tgtctgtcaa cactaaattt cataagtcat ttcaattggt
      421 taattaatct gcaaagttgt taattaatga acattcgtcg ccgaaaacga gtctgtatca
      481 ttagcgtgat gcagtctctg caaaggatct tgttgataaa attggcagaa acgttgccac
      541 aatgaaggga aacgaggagc aaaaagttct ggggcgctaa agaaatattc agaaagtacg
      601 gcaaaacatt cagcaggatc gctggctgca taagcatcaa tgctcgccgc attctcgcca
      661 accaattcga tttcttcctg aatgttgttc attgcagcat gaagatcgtg ttcccagcca
      721 gcaacctcac gcaacggaat aaagggaact ccgctggcgc gatcgccgtt acgggtgtcc
      781 agcttatgag cgacttcatg aataatcagg ttaaaaccag atgcatcaaa agaatcttgt
      841 atatccaacc agttcaaaac gataggccct tgctgccagc tctgacctga ctgaacaata
      901 cgttggttat gcaccagacc gatatcgtct tcccattcat catcgaccac aaatggcgca
      961 ggataaatta agacttcatg aaaaccatcc agccattcca gtcctaactc cagaacgggt
     1021 aggcaaaata gaagtgctat ccggcagctt cttaatgaat ccagttcaaa gccctgtaaa
     1081 ggaacaagcc gcttttgctg taaaaaacgt tcggcaagag tgactaattt gctttgttcc
     1141 tgttccgtca gacccgttaa aagggggatc gatagtgctt cctgccaggg aagggcagtt
     1201 tgatgtgctg attcttgtac tttccagggc cacttaatca tcgttttgct cgcaaactcg
     1261 tcacttgaac aaaattgcac ggacagggac tgttaaaatg ccaaatttcc tggcatcatg
     1321 gcaaccatct gaacggagag atgccggagc ggctgaacgg accggtctcg aaaaccggag
     1381 taggggcaac tctaccgggg gttcaaatcc ccctctctcc gccactttat caatgactta
     1441 tctcctgact tcccgccttg cttttcctaa acagaacaat tgtagaatat tcttgaaggg
     1501 ttagatcgtc actgttttct gttcgatact gtgacattca gcacttgatt cgctatggat
     1561 ctgacaggaa gctttcgagc gaaaatgtgc agttattcag tcgttttctt atcgctcacc
     1621 attattcttt tagacattca tcctatgaaa ctgccgcaaa gttggtggtg ggaactgaag
     1681 ttgcgtagag aaggggtcaa taattgagtc aaaaaaggaa cgcggccgtc agattaaagc
     1741 cgcgggacaa agtccataat ttccaagagt tgttgaattt cccagctttt ttgaaagcct
     1801 ttgtatcata ctaacaagta aaaaaggttt aaataccaaa attaacctgt atttttgata
     1861 agtcttacca ttgtacgatt tacacagttt ctgctcctcc ttgttgatag tagaaaggat
     1921 gcattgtgca cttgcgtaaa catttatgac gcgttgatgt acatggttca catttccttg
     1981 tcaggggatc gacttatttg cggcgatgca tcattcgtaa cagagtatta tctttctgaa
     2041 tgtagtgatg aaagagtgct gcgccagcgt gagctgcgat taaaaagtaa cctatatttg
     2101 cctaggtttc gtgaatattt tttatcagtg ctttaatttc gctgtttggg gtaacaaagg
     2161 gagacacatt gaaaccaagg aaactccacg attttccact gtaagccatc aaagcaatcc
     2221 ccagcagagg aagtgcaagg aaggttatat aaagcatgat gtgcattagc gaagccgctt
     2281 tcatttgcca ggcaggtggc ggtggtacaa tagaaggatc actataacgg tgttttataa
     2341 tcaggcgtga aaacattaac acccaaacga atatacccgc attgtaatgt gtttctcgca
     2401 tcagcagata agtactacta cctttaggaa accagccacg gagttccatc gcggcatagg
     2461 ttattgcaac gaaaagcagc gttatccaat gtaaataaat ttgagttttt gagaatcggt
     2521 tcatttttgt agctcctgta atttttacag tcaagctatc ccacgaacct taaaaattcc
     2581 ttatacagat gtggttttaa gaatttgtta tttaaaaaat tgatagtgca cattgtaacc
     2641 agccaatata agcaatggcg tgagcacagg tatctttctc tgttggccgt attgtgtatg
     2701 gaatccgtta ttggttaact caatgcgggt tagcttatta atatacagac acgtcatagt
     2761 gatgagcgtg tctgtagcta gcttgcgttc agtggcgttt gtggatggac cagtggcagt
     2821 tatgatgact tactaaagcg gcatcgaggc gttatcatga gaatactcaa tgagacatca
     2881 tttcctcgac cacttcttcg ctactcaact gatatggata atacgttggc cagttttcca
     2941 tttcatttaa taaaaattgc tgtgaatcat tacccataaa aatgtgaaaa tgagatgatt
     3001 ttcgtggtgc aattatatga tcgctaaatt gtatatattt aggggctttg ctttcaggat
     3061 ctttacattc gaataagtaa cgaacgcctt tcttgcctga tttataggtg agtattttgt
     3121 atccatcgta atcatattta caggatgttg tttcattatt tctatggaat tcaacaatgc
     3181 cgtcctcaat gccaatcatc tcgatatctg ttgcataacc tttgtgataa taatctttaa
     3241 tttcagcaaa tgttttaatt ttatctgcat ccgctttctt ctgaaagacg gggtcaagtt
     3301 tgccactttg cagtaaagga taaacggatt gccagactcc atcccagtca ctgagcgttc
     3361 ggttttgtac attggcatca tcaaaaacac cattagcagc tttttgttcg acctctgtta
     3421 agggtttgcc gtgtgagtga tgaccatgcg aaaaggcagg agcgctaaca ataaagacac
     3481 ctaaagcaac agccagtttg tgaagacgaa ttgccaaaac agtttcctcc agagtcattg
     3541 tatatgatgt gtaatgttat attgtaacat atggcaaaat gcaacaactc ttaggagatg
     3601 agagcatagt tttctcaggt gtacttatct atcttattga aataaaatga attatgttga
     3661 taatttgagg ttgatgacaa acgaaacata acctgatgtg atctgcgcag gccggacagg
     3721 gcgctcgcgc cacatccggc agggataacg cgtactttgt cggcaatatg aagttataca
     3781 gataatttgt tgtgaacctg cttgcgtaac cgcttaaaaa gggaacgcaa cttcttatac
     3841 cgtaaggcta aaagcagtac agccagccca gcgtagatga gcggctgcgg tgaaataatc
     3901 ttcactgacc acagataatg tattggggcc aggatcgcga caagatagac gaaattatgc
     3961 aactgttgcc agtgcttgcc gagttttcgc tgcatcgcct gggttgaagt aaacgctaaa
     4021 gcaagcaaaa taacccagct gataattcct aacgttaaat aaggccgggt aattaactcc
     4081 ttgcctaata acgccagatt attcacgccc agctccagca atgcgtaact ggttaaatgc
     4141 agtgtcgccc aggcaaagca ccataagcct aacaggcggc gagtgcgtat caataacggc
     4201 tgttttgcgt agcgtgccag aggtgtgatt aacaaggtcg ccagcaaaaa tttcagtgca
     4261 gtgcgaccgg taaaatgctg aatatctttc accggatcgg cacccagtcc accgtgattg
     4321 atcgcccaga ccagccagag aaacggcaac aatccggcaa gatgcaggct aactttcagc
     4381 catgtcacct gttttgctgt cagacgcatt cagaaattct cccgcaaatc caggccacga
     4441 tacagcgatg ccacctggtc ggcgtaacca ttaaacagta gcgttggctg gcgctgaaca
     4501 tcgaggatgc cgcctgaacc aataaatcgt tcggtagcct gtgaccagcg cgggtgatca
     4561 acatgcggat taacgttggc gtaaaaaccg tattcgtcag gcgctgccag attccaggtg
     4621 gttggcggac gctcgcgggt cagcttaata ctgactatcg atttaatccc tttaaagcca
     4681 tatttccacg gcacaatcag tcgtaccggc gcgccatttt gtggcggtaa cgccttgcca
     4741 taaacaccca cggtcatcag tgtgagcgga tgcattgctt cgtcgagacg caatccttcg
     4801 acataaggat atttcagccc gccgccgata aagcggtcct gctggcctgg catttgttcc
     4861 ggtgcataaa ttgtttcgaa agcgacatac ttcgcattgc tggtgggttc ggcaagcgcc
     4921 agcaatttgt gcagcggaaa accaatccac ggcaccacca tcgaccatgc ctccacgcag
     4981 cgcatacgat aaatacgctc ttccagcggg aagcgacggg ttaaatcatc gtgatcgagg
     5041 gtcaatggtt ttgccacttc gccgctgatt ttcagtgtcc atggatcggt tttcaggcta
     5101 ccagcattag cggcgggatc ggctttatcc agcccgaatt catagaagtt gttataaccg
     5161 gagactttat ctactggcgt cagtggcaga ttattttgcc aggcggtagg cttgctgaac
     5221 tccagcggtt ttccggcggg ggcgggcggg cgatcgttcc ctttaaacca gctaagcaga
     5281 tcggcatgcg cagcgtgagg caaagaaagt gcagctgcgc tgatgcccag tgcttttaac
     5341 acctgtcgac gcttcataaa gaataccgac tcggccgtaa catctgattc ttttaaaaat
     5401 tgattctttt tcataagcca tcacactttt ttgtcgttag ccagcaagag ccgtttactg
     5461 ttaatctgtc agggagtgtc ctccctaaca gtagcagaga tctgctttta catatttatg
     5521 cattaactgc cacgataggt tgaataccca tattggctaa gtaataaagg cacatgataa
     5581 tgctcgttca ctttattaat atgaaattca acggggatct cagggaagaa actttcaaga
     5641 ttttgtttct tgaaatagtc cccggtttta aatacgacac ggtaatcgcc cgtagttgca
     5701 gtttgctcgg gccacagtgc cttaattcgt ccatccttat ctgttttggc ggtattaagt
     5761 tgtaaccagc cgttgtccgc tttcttttca agagtgactg tcacgtcggc agcgggtttt
     5821 ccggtttgct ggttcaaaat gtgcacgcta agaatgtttt gttgtgcggc ataaaccaaa
     5881 gaaggtaatg aaaatgctgc cgttactacg gagagtacta aataacgctt taacatgttt
     5941 atatccttgt catgtgaatg agttttgagg atagcgaaaa agcggccagg gtaaccttac
     6001 agcagcatta caattttgta atgaactagg ctgtttctat aacatatgat ttatggcata
     6061 ttattttcat gaagatttta cttattgaag ataatcaaag gacccaggag tgggtaacgc
     6121 aggggctttc cgaagcgggt tatgtcattg atgccgtttc tgatggcaga gatgggcttt
     6181 atcttgcgct gaaggatgat tatgcattga tcattctgga tattatgctt ccgggtatgg
     6241 atggctggca gatcttacaa acgttaagaa cagcaaagca aacccctgtt atttgcctta
     6301 ctgcaaggga ttctgtcgat gacagagtca gagggctgga cagtggagca aatgattatc
     6361 tggtaaaacc tttttcattt tctgagttgc tggcaagggt tcgggcacaa ttaaggcaac
     6421 atcacgcttt gaattcaaca ttagaaatca gtggcttaag aatggactct gtaagtcaaa
     6481 gtgtgagcag gaacaatatc agtattacac tgacgcgcaa ggagtttcag ttactttggc
     6541 tactggcctc cagagctggc gaaattatac ccagaacggt tattgcgagt gaaatttggg
     6601 gaatcaactt tgatagtgat accaatacgg tggatgtcgc cattcgcagg ctccgcgcaa
     6661 aagttgatga tccttttcct gaaaagctaa tcgccacaat ccgggggatg ggctattcat
     6721 tcgtagcggt aaaaaaataa tgaaaagatt atctataacc gtccgtttaa ccttgctttt
     6781 tatattgctg ctgtctgttg ctggcgctgg aattgtctgg actctctata atggcctggc
     6841 aagtgagttg aaatggcgcg atgatacaac actcattaac cggacagcac agatcaagca
     6901 gttgttaatt gatggggtaa atccagatac gttacctgta tactttaacc ggatgatgga
     6961 tgttagtcag gatatcttga tcattcatgg tgatggcatc aataaaattg ttaaccggac
     7021 aaatgtcagt gatgacatgt taaataacat acctgctagt gagacaatca gcgcagctgg
     7081 catttacaga agcattatta atgatactga gattgatgct ttacgaatta atattgatga
     7141 agtttcgcca tcattaacgg ttactgtggc taaattggct tcagccagac ataacatgct
     7201 tgaacagtat aaaattaata gcattataat ttgcattgtc gccattgtac tttgctcagt
     7261 attaagtccg ctgttaatca gaacgggatt acgagagatc aaaaagttga gtggtgtaac
     7321 ggaagcgctg aattataacg atagccggga gcctgttgag gttagcgcat taccgagaga
     7381 actaaaacct cttgggcagg cgttgaataa aatgcatcat gctttagtca aagattttga
     7441 gcgtctaagt caatttgctg acgatctcgc tcatgaactt agaacgccca ttaatgcatt
     7501 actgggtcag aatcaggtta cgctcagtca aaccagaagt atcgctgaat atcaaaaaac
     7561 aattgccggt aacattgaag agctggaaaa tatttcgcgg ttaacagaga acatactgtt
     7621 tcttgcccgg gcagataaaa acaatgtttt ggtgaaactg gactcgcttt ctctcaataa
     7681 ggaagtcgaa aatttgttgg attatcttga atacctttca gacgagaaag agatttgctt
     7741 taaggtcgag tgcaatcagc aaatctttgc ggataaaatt ttactgcaac gaatgttatc
     7801 gaatcttatt gttaatgcca ttagatattc accagaaaaa tcgcgtattc atataaccag
     7861 ttttcttgat accaacggct atcttaatat tgatgtcgcc agtcctggaa cgaaaattca
     7921 tgagcctgaa aaactcttcc gtagattttg gcggggagat aattcgcgtc attccgtagg
     7981 tcagggactt ggtctttctt tagtcaaagc gattgccgaa ttacatgggg gaagtgctac
     8041 gtatcactat ctcaataagc ataatgtgtt ccggattatg ttaccgcaaa gaaattaaca
     8101 tagggcttca gtacgccata aatcaaccat gaaaaaacgc agccacgtcg tgagtattaa
     8161 cgtggctgcg attgattatg cgtttacatt caaacgtaac agggattaat ccgcataagc
     8221 tgccagcatt tcctgcgccg ccagtttacc caacgcattc gctgcgaaag gactgtcgcc
     8281 ggtgagaact ttacggtcct tatgtactcg cccggtgatg tcgtcattaa taatgttcat
     8341 gcccattttc ttcagctctt cgccgaagta ccaggtgaga tgacccggca tatagcctat
     8401 ctctggcgtt tgtttgtctg cggcgtctgg gaatgcgcaa atggaataac cattcagtgg
     8461 gttatcgccg tggcgaagcg ccagaaaagc cgccgggccg tggcaaaggg agataacaaa
     8521 acggtcattt ttgattgccc actgcaaagc agcagctacg tcctggcttt ctggtaaacc
     8581 aataagtgcg ccatgaccac caggaacaaa gattgctgca tattcgctat cagcgttgag
     8641 gctggcgaca acatccgcga gtttcttcgg attgcggaac aacgatttat gctgctcaaa
     8701 gaatggcatt actttttcat ctttgtgcgg catagcccag tattcaaact tggtcatcag
     8761 accggaaatg gtcgccactt cgaattcgaa accagcagca tggagatgat acaacggcag
     8821 caacgtttca atcggatggt taccggtcga gaacagtttt ccgttatcgg ttggcaaata
     8881 acgttcgtcc gcggcgatca ccagaatttt atgcttaccg cgatacggtt ttggatagtc
     8941 cacgccatca agatcagaga cgggactggt atattggcta agcgaatatt ctgaagggaa
     9001 gaatgcatta tcttcagcaa tatcgacctg cggattttta cttgtttgaa cagtcatagt
     9061 gtattcctta ttgttgctta gttagggtag tcactatatt tgcgactgag ctggtcgcta
     9121 tttttaacca gtctggaaaa ttgagtcaat atatcgaaag cggtagtcgg cgggtgtaag
     9181 tatgtaatga tgatgctgca ctggtgttga atattattca cgccaaattt tccttattga
     9241 gcaacgtcgt cgcaagtggc ctcaatgatt ttagaccatt atcaaaggta cggggcggcg
     9301 gggagagatt tagtgctgtg aaatattgtg gtactgatga atatttatat cgcggattgg
     9361 tagagaaaag attatttcat gcactatttt tgcaaatatt tctatatggg aagcgtgttt
     9421 gttacggatt aattcataaa gaaatgcatt tgggtgttgg tttgaagagt cgctggttga
     9481 tgttattgtg ccaggagggg cagggtaagg tgctgtatcg atatgcattg gcaattgcgt
     9541 gtgccgcaga tggagatgaa atgccaataa aaaagcctga attcgatgag ggacgaactc
     9601 aggctagtgt ttagttttac agtaacacgc cgcagcacgt tggctgatta gaactggtag
     9661 actaagccca caccaacaac gtcatctgtt gcgatgccat tgttgtcata gaaatcgtca
     9721 tctttatcca acaagtttat tttatagtca acatatgttg agaagttctt attgaaatag
     9781 taggtcgctc caatttcaac atagttgacc agatcctgat caacatactc ttgatgacca
     9841 ttgttgttat agtcgttacc gccgaggtct ttgccttttg aataaacata tgcaagagat
     9901 gggcgcagac caaaatcgaa ctgatactgt gctacagctt caaagttctg ggttttattt
     9961 gcgataccca cattaccata tggtgtcata ttgcgggttt cagcatacat cattgc
//
LOCUS       DAZPLI010000141         9990 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZPLI010000141 DAZPLI010000000
VERSION     DAZPLI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9990
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.142"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            2..895
                     /locus_tag="ACHUU3_003376"
     CDS             2..895
                     /locus_tag="ACHUU3_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR2083365.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            1030..2250
                     /gene="mlc"
                     /locus_tag="ACHUU3_003377"
                     /gene_synonym="dgsA"
     CDS             1030..2250
                     /gene="mlc"
                     /locus_tag="ACHUU3_003377"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR2083366.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            2375..3070
                     /gene="bioD"
                     /locus_tag="ACHUU3_003378"
     CDS             2375..3070
                     /gene="bioD"
                     /locus_tag="ACHUU3_003378"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR2083367.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(3023..4315)
                     /gene="clcB"
                     /locus_tag="ACHUU3_003379"
     CDS             complement(3023..4315)
                     /gene="clcB"
                     /locus_tag="ACHUU3_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFR2083368.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(4474..5088)
                     /gene="dmsD"
                     /locus_tag="ACHUU3_003380"
     CDS             complement(4474..5088)
                     /gene="dmsD"
                     /locus_tag="ACHUU3_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR2083369.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(5131..5985)
                     /gene="ynfH"
                     /locus_tag="ACHUU3_003381"
     CDS             complement(5131..5985)
                     /gene="ynfH"
                     /locus_tag="ACHUU3_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR2083370.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(5989..6603)
                     /locus_tag="ACHUU3_003382"
                     /pseudo
     CDS             complement(5989..6603)
                     /locus_tag="ACHUU3_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
     gene            complement(6614..9037)
                     /gene="ynfF"
                     /locus_tag="ACHUU3_003383"
     CDS             complement(6614..9037)
                     /gene="ynfF"
                     /locus_tag="ACHUU3_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFR2083371.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVEYDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPAYTPDFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(9098..>9990)
                     /locus_tag="ACHUU3_003384"
     CDS             complement(9098..>9990)
                     /locus_tag="ACHUU3_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HFR2083372.1"
                     /translation="ADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPI
                     YWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRK
                     DPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYAS
                     TFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIAT
                     GDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLR
                     PSPLAKGNPQHTNLVEIEKI"
BASE COUNT         2449 a         2553 c         2593 g         2395 t
ORIGIN      
        1 tatgaatatt gaacttcgtc atctgcgtta ctttgttgct gttgcggaag agctgcattt
       61 cgggcgcgcc gctgcccgcc tgaatatttc gcaaccaccg ctaagtcagc agattcaggc
      121 actggagcaa caaattggtg cccgactgct ggcacgaact aatcgcagtg tattgctgac
      181 ggcagcagga aaacagtttc ttgcagatag tcggcaaatc ctgtctatgg tagatgacgc
      241 tgccgctcgc gctgaaagac tgcatcaggg tgaagcgggg gagttgcgca ttggttttac
      301 ttcgtccgct ccttttattc gggcggtgtc cgatacgtta tcgctgtttc gccgtgatta
      361 tcccgatgtc catttacaaa cccgcgaaat gaacactcgt gagcaaatcg ctccgctcat
      421 tgaaggaacg ctggatatgg gattgctgcg taacacagcg ttaccggaga cgcttgaaca
      481 cgcagtcatc gtccatgaac cgcttatggc gatgatcccg cacgatcatc ccctggcaaa
      541 taacccgaat gtaacgctgg ctgaactggc gaaagaaccc ttcgtctttt ttgatccgca
      601 cgtcgggaca gggctgtatg acgatattct cgggctgatg cgacgttacc atttgacgcc
      661 cgtcatcact caggaggtgg gcgaggcaat gaccatcatc ggtctggttt ccgccggtct
      721 gggtgtttca attttgcctg cttcatttaa acgtgttcag ctcaacgaaa tgcgctgggt
      781 gccgattgct gaagaggatg cggtttctga aatgtggttg gtctggccga aacatcatga
      841 acaaagtcca gcttcgcgta actttcgtat tcatctgctg aatgctctca ggtgagggaa
      901 atttcagcga aaaagcccga aaaatgtgct gttaatcaca tgcctaagta aaaatttgac
      961 gacacgtatt gaagtgcttc accatagcct acagattatt tcggagcgcg aaaatatagg
     1021 gagtatgcgg tggttgctga aaaccagctt gggcacattg atcaaataaa gcagaccaac
     1081 gcgggcgcgg tttatcgcct gattgatcag cttggtccag tctcgcgtat cgatctttcc
     1141 cgtctggcgc agctggctcc tgccagtatc actaaaattg tccgtgagat gctcgaagca
     1201 cacctggtgc aagagctgga aatcaaagaa gcggggaacc gtggccgtcc ggcggtgggg
     1261 ctggtggttg aaactgaagc ctggcactat ctttctctgc gcattagtcg cggggagatt
     1321 ttccttgctc tgcgcgatct gagcagcaaa ctggtggtgg aagagtcgca ggaactggcg
     1381 ttaaaagatg acttgccatt gctggatcgt atcatttccc atatcgatca gttttttatc
     1441 cgccaccaga aaaaacttga gcgtctaact tcgattgcca taaccctgcc gggaattatt
     1501 gatacggaaa atggtattgt acatcgcatg ccgttctacg aggatgtaaa agagatgccg
     1561 ctcggcgagg cgctggagca gcataccggc gtaccggttt atattcagca tgatatcagc
     1621 gcatggacga tggcagaggc cttgtttggt gcctcacgcg gggcgcgcga tgtgattcag
     1681 gtggttatcg atcacaacgt gggggcgggc gtcattaccg atggtcatct gctacacgca
     1741 ggcagcagta gtctcgtgga aataggccac acacaggtcg acccatatgg taaacgctgt
     1801 tattgcggga accacggctg cctcgaaacc atcgccagtg tggacagtat tcttgagctg
     1861 gcacagctgc gtctcaatca atccatgagc tcgatgttac atgggcaacc gttaaccgtg
     1921 gactcattgt gtcaggcggc attgcgcggc gatctactgg caaaagacat cattaccggg
     1981 gtgggcgcgc atgtcgggcg cattcttgcc atcatggtga atttatttaa cccacaaaaa
     2041 atactgattg gctcaccgtt aagtaaagcg gcagatatcc tcttccctgt catctcggac
     2101 agcatacgtc agcaggccct tcctgcgtat agtcagcaca ttagcgttga gagtactcag
     2161 ttttctaacc agggcacgat ggcaggcgct gcgctggtaa aagacgcgat gtataacggt
     2221 tctttgttga ttcgtctgtt gcagggttaa cattttttaa ctgttctacc aaaatttgcg
     2281 ctatctcaat ttgggccagg aaagcataac ttagactttc aaggttaatt attttcctag
     2341 tttatatttg tgaagcataa cggtggagtt agtgatgctg gagcgtttct ttattaccgg
     2401 tacagacact tctgtaggga aaacggtggt ttcccgcgca ttgctacaag cgttagcctc
     2461 ccagggaaaa acagttgcgg gatataaacc cgtagcgaag gggagcaaag agacacccga
     2521 agggctgcgt aataaagatg ccctggtgtt gcagagtgtt tcaaccatcg aactgcctta
     2581 tgaagcagtt aatcctatcg cgttaagcga agaagaaagt agcgtggcgc acagttgccc
     2641 aatcaattac accctcattt caaacggcct ggcaaacctg accgataaag tcgatcatgt
     2701 cgtggtagaa gggactggcg gctggcgcag tctgatgaat gatttgcgtc cactctctga
     2761 atgggtggtg caggaacagt tgccggtgtt gatggttgtc ggtattcagg aaggttgcat
     2821 taaccatgca ctgctaacag ctcaggcgat tgccaacgac gggctgccgc tcattggctg
     2881 ggtggctaac cgaatcaacc caggactggc gcattatgcg gaagtcattg atgtgctggg
     2941 taaaaaactt ccggcaccgc tcattggtga actgccttat ctgccgcgcg ccgaacagcg
     3001 agaactgggg caatacatcc gcttagctat gctgcgcagt gtgctggcgg tagatagagt
     3061 cacggtgtaa cgtccgcgaa attaccgacg caatgacgca ggcaatcaat aaaccgggga
     3121 gtagctgata ctccccggtc atttcacata tcatcaacgt cgacataatc ggcgcgtgcg
     3181 tggttgccgc caacagtgtc gccatcccgg tcaatccgag taaaagcgta atttcttcgc
     3241 catcagggaa ccataatccc aggctacgac catacaacat gccaatggca agaccgataa
     3301 atagcgtcgg tgtaaagaca cctccgggtg cgccggaacc gctactcgcc agcacggcac
     3361 acagtttaca aaggaagatc ccggcaatga tcattaacag tggtggggcg gttaaaaagg
     3421 attgtacggt gctatagccg ttgccccaca ctgcaggtgt gaacagggaa agcagaccca
     3481 cgatcaaccc gcctagtgcc agttgccagg gcggcgcaag tttgagactc acaaatccac
     3541 gatgacaggc gttcattaac gttaacaaca gtggtccaca cagacctgcc agcacacctg
     3601 tactgataat cagcgcatag tcactagcct gaaccgttac tgagagttgt acgctgtaga
     3661 gtaacgcgtc gctatgatta ataagattgc taaccagcaa cgccacgacg gcggaaataa
     3721 tcaccgggcc gagagaggcc aacatcatag tgccaaacag cacttcggct ataaataaac
     3781 tgccagcaag cggggcgcga taggccgcag ccattcccgc cgcggcccca caggcgatcc
     3841 ataatttcca ttcctggcgt ggcgtaaaac gttgggcaaa acaggaggcg gcaagggcag
     3901 ctaaaagaat catcgcacct tcgcgaccaa ttgcactgcc gctggttact accagcagcg
     3961 aggcaagcga tttaaccagg cttgctgcgt aatcgaactg tccatcggtt tgcaacgctt
     4021 ccatgtaatc ggtcggcgca tgagggcgtt gttgggtaaa tttctgccag cccatcagca
     4081 acaaacccgc cgccagtccg ccgagcgccg gagttagcag ccgtcgccag ggggaaaggt
     4141 ttgtcgctgc attgaccaga ctgccggagt cattattgag gaacaaccac tccagtagca
     4201 gcatcgcatg acgaaacccg gcaacggcaa aggccgcgag aataccgacg actgttgcga
     4261 taagcagacg gcggaacata gtgcgcagat ccggatagat gtgtagatga tgcatgagca
     4321 gaaatgggca atgaaacacg ggcgactatt ttgcggcaaa agaggccaac cgcaaaagca
     4381 gtgcgtgctt taagagataa aaaaacgtga cattgtaact tattattgcc gaatcgcgcg
     4441 taatcgtcac catccggcaa tattacggtg atcctatcga aagagtggtt taaccgcgac
     4501 aggaatcaac agttgtgatt gccattgcgc cagcgttaag cgagccagtt cacccagtgc
     4561 acggtagaaa gggtgttctg ctttttcgat aaaaacatca agaaaacgtg ttgaccacgg
     4621 aaaaaggtgc catgccagca attcttcgca ttctgtctgg cgaccattct ccgccaacca
     4681 cgcagccatc aacagcagtg aaccaaaatg atcttccggt tcgttttgct tcatttcaaa
     4741 ctgaatgcct ttctcgcgca tccactgacg aagtgccaat gttgaatcgc caaacagcac
     4801 agattcgcga tccagccaga ccgatcccca cggcggagac ggcagtgccc acgggccgac
     4861 aaacaaacgc tgccaggcct gggcgtgagt ctcttcacac tgtgtctgaa aagccgtcac
     4921 cagcggcgct aatgacgctt ctggtagagg ccactgagtt tcccagccgt cactggtgag
     4981 taccgcaacc agaggtgcgg cttccgcact ctctggagcg taataaaaca gcgcgcccaa
     5041 cacgcgcgcc gcgacagaaa aattatcttg ctgtgaaaaa tgggtcattc ctaacatcct
     5101 gaaatgtgcg ggttgccccg caccaatttg ttaacctgca atagccattc ctactgtcat
     5161 gtgcaggcca taaaagagca cacgcccgat catctcacca acaagtatga ggattagccc
     5221 cagaacaagt ccggcaacgt gaggttcacg gcgacgaatg agcggacaaa gccagcatcc
     5281 aagacctgcg cataacaata ccacacgcca gacctgtaag gaggcgtaat ctggtaccag
     5341 tgcgctggct tgttgcacgg atgagtgaat agacgccaga gacaaacctt gcagcacaat
     5401 caccccggca caagcgatca gcgcgagaac gctaatgatg gcaaatggtg tggtattaaa
     5461 tgtaacgcgg gctgcccgca gaattgcggc agcaagtatt ggaccgctca acaataccgt
     5521 caggaagaaa gccagcgtag tgtaaccgtt atgccaggtt ggcacagtgt cgatttgata
     5581 cacgcaggtc atcatccaga cgaaaatgac gccgagcgcc atgctgacaa gtaaccagag
     5641 tttccccaac gcttgcggca ttttaccgat gaccgccacc agccaccata gtccgccaac
     5701 ggcaaaaaat atggaaccgg cagcaatctc gttactcaga ccagaagctc cgattcgatt
     5761 cagcgagtta aacgcccgca gtggcgatcc caggtgcatg acagaggcca taaacccgat
     5821 gcccatcaac agccagagga aaaacatgcc acggacaata cgctgacggt ctgcgtcatt
     5881 tttggcggcg aaccagccaa ttccgctaac aatcagcgcg cctactacgc attgacccaa
     5941 tacggtaaag ataaccagtg gccactcatg ccatccattt cccattttaa cttcctccgg
     6001 attagccaga taaccagtgg tatcgctggt cgggcggctg ttggcgttag gtttgataac
     6061 gatattgggt tttgtgaaat gcgcgcgcgg cagtggcgcg acggcagcca gagtgccgtg
     6121 cttctggcgc agctcttcaa ttggaccgaa ctcaagtgca cgcagcgggc aggattcgac
     6181 acatatgggt tgtttcccct cggcgacgcg cgaataacaa ccatcgcact tcgtcatgtg
     6241 ccctttttca gcattgtact gtggcgcgcc gtacggacaa gccatgtggc agtagcggca
     6301 gccgatacaa acatcttcat caaccaccac aaagccatct tcacgcttat gcattgcgcc
     6361 gctggggcag acttttgtac atgcggggtc atcgcaatgg ttacaggaga tggagagata
     6421 ataagcgaac acgttctggt gccagacgcc attatcttcc tgccagtcgc cgccagcata
     6481 ttcataaata cggcggaaac tgacttccgg gccaagatct ttaaaatctt tgcacgccag
     6541 ttcgcaggtt ttacagccag tacagcggct ggaatcgata aaaaatccat attgtgtggt
     6601 cacgggctac tccttaaacc ttttcgatct ggacaagatt gctgtgcgac gggtttccct
     6661 ttgccagcgg tgaagggcgg tgagaggtca gaatattgat actgccgccg tgatcgaccc
     6721 ggtcaccaaa catatccgct ttaagccaag caccttgccc gatggcggta acgccaggca
     6781 gaatacgcgg agtcactttt gcggcaatca gcatttctcc attattgtta aatacccgca
     6841 cggtatcgcc atgacggata ccgcgtgcct gagcatcaat ggggttgatc cacacctctt
     6901 gtgggcaggc ctgctgtaac acatcaatat tgccgtagct ggagtgggta cgcgctttgt
     6961 aatggaagcc cgttaactgc agtggatagg ttttccgcag gggatcgtcc cagccatcaa
     7021 aatctggggt atacgcagga aggggatgaa taacttcatc ttttttcaat tcccaggtat
     7081 ccgcaatctt cgccagtcgt tcagaataaa tttcgatttt ccccgaaggt gttttcaacg
     7141 ggtttgcctg tggatcttca cggaatgcgc ggaaagcgac gtagtgttct tccgggcatt
     7201 ttttcttaaa gatcccggtc gttttcatct cctcgtagtc gggcatctca gggttacgtt
     7261 ccttcgtttt cgcatggaga tatttgatcc attcatgctg actgcgacct tcagtaaagg
     7321 tttgataaac gtctggtcct aagcgtttgg cgacttcact cagcatccag tagatgggtt
     7381 tgcgttcaaa ttttgctgag gttgcgggtt gggcgaggat cacatagccc atattccctg
     7441 cagattcatg agagataagg tcttcttgct ctgttggcat caggtcgggc aacaggatat
     7501 cgcaatactt agccgaggcc gtcatgaagt ggtcaatgcc aacaatcatc tcgcacttgc
     7561 tgtcatcctg aagcacctca tgggtgtgat tgatgtcgcc atgttgattg atcaatgtgt
     7621 tactggcgta gcaccataaa aacttgatgg ggacatccag tttttctttt ccacgaacac
     7681 catcacgggt cgcggtcatt tccgtaccat ggtcgatggc atcagtccat gtaaagacgg
     7741 aaatctgcgt tttaacagga ttctcgagca tcgggaacca ttctaccccc agatcccagc
     7801 taccttcgcg tacgcctgag ttgccgccgt ttatgccgac gttaccggtg agaacggaaa
     7861 gcatggcaat agcgcgggat gtttgttcgc cgttggagtg tcgttgtggc ccccaaccct
     7921 gacaaatata agcaggtttt gctgaaccga tctctcgtgc caactggata attttttctg
     7981 ccgggatgct ggtgattttt gctgcccatt ccggcgtttt agctatgccg tcaggccctt
     8041 cgcccagaat ataggcttta taatgcgcgt tacgtggtgc gttggcgggc agcgtttttt
     8101 catcgtaacc aacacaatat ttgtcgagaa atggctgatc gaccatgttt tcagtaatca
     8161 gtacccaggc aatcgcacag gccagtgcgc catcggtgcc agggcgaatg ggcagccatt
     8221 catcttcacg cccggcagct gtgtcgttat aacgtggatc gatgacgatc atgcgtgcgt
     8281 ttgaacgttc gcgggcttgc tcgacgtagt aagtgacacc accaccgctc atccgcgttt
     8341 ctgccgggtt atttccgaac ataacgacca gtttcgtatt ggcgatatca tccgggctgt
     8401 tgccatcatt ggcaccgaac atataactca ttgcggcact gatctgtgcg gtactgtagc
     8461 tgccatagcg actgagaaaa ccaccgcaag agttcatcag acggtacggg acgtttgagt
     8521 tggtgatgtt tccgccatct acacctgttc cgtacaggac atgtacagct tcattgccgt
     8581 aatcttttag gatccgccga agattatcac tgatggtatc cagggcttcg tcccaactta
     8641 tccgttcaaa tttaccttca ccgcgcttgc cgacgcgctt catgggatat ttcaacctat
     8701 caggatgatt catccgtcgg cggatagagc gcccgcgtaa acacgcgcga acctgatgat
     8761 taccgtagac gtcgtcacct gtcgtatcat actccaccca gtacacggtg tcatctttca
     8821 catgcaaacg taacagacag cggctcccgc agttaacggt gcaggaactc cagaccgctt
     8881 tctcttctac cggagcctct gccgcccgaa ccatttggga aaatggcaga gtgaaagcac
     8941 tgcttgccag cgcaaggctg ccaagtgcgg aggttttcat cagacttcta cggctgattt
     9001 cagccttcat gagcgcctct gtggtatgga ttttcatcat tactcactta ttgcttttca
     9061 aacaaaatgt catgccagaa tttatggttg tagtgggtta tattttttcg atctcgacca
     9121 gattagtgtg ctgcgggttt cccttcgcca gtggtgaagg gcgcagagtg gttagcgtat
     9181 tcacacagcc gccatggtcg attttatcgc cagacatatt ggcctcgtgc caggctccct
     9241 ggcccatagc gctaacccca gggagaatgc gtggtgttac tttggctggt agccgaactt
     9301 cgccacgatg gttaaacacc cgcaccatat cgccggtggc aatcccacgt ttctgtgcat
     9361 ctatagggtt gatccacact tcctgacggc aggctgcctt caggacatca atgttgccgt
     9421 aggtcgagtg agtacgggat ttgtaatgga aaccaaacag ttgcagtggg aaggtactac
     9481 gttcagggga gtcccagcct tcaaaggttg aggcataaac tggcagtggg cttatcactt
     9541 catctttttc cagttcccag gtacgggcaa tttccgccag cttgctggaa taaatttcaa
     9601 tcttaccgga aggcgtttta agtggatttg cctcggggtc gtcacgaaat gctttgtagg
     9661 cgacaaaatg accattggga tctttacgct tatagatacc catttttttc agttcgtcgt
     9721 aagacggtaa cgccggatct ttggcaagca ttttggcgta cagatgttgt aaccattgtt
     9781 cctgcgtgcg accttcagtg aacttttgat agacgtcagg tccaagacgt ttcgcgactt
     9841 cactcaggat ccagtaaatc ggtttgcgtt cgaatttttc gctggtgaca ggctggagga
     9901 aaatgagata tcccatgtta ccggcgtagt cgttaggaat aatatcttcc tgctcaacgg
     9961 tcatcaggtc tggcagcaga atgtcggcat
//
LOCUS       DAZPLI010000142         9949 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZPLI010000142 DAZPLI010000000
VERSION     DAZPLI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9949
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.143"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(124..714)
                     /locus_tag="ACHUU3_003385"
     CDS             complement(124..714)
                     /locus_tag="ACHUU3_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR2083373.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRIADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGAAQQSPEHRS"
     gene            complement(752..961)
                     /gene="hha"
                     /locus_tag="ACHUU3_003386"
     CDS             complement(752..961)
                     /gene="hha"
                     /locus_tag="ACHUU3_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR2083374.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRNSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            complement(1007..1468)
                     /locus_tag="ACHUU3_003387"
     CDS             complement(1007..1468)
                     /locus_tag="ACHUU3_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR2083375.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLINIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            complement(1713..1925)
                     /locus_tag="ACHUU3_003388"
     CDS             complement(1713..1925)
                     /locus_tag="ACHUU3_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR2083376.1"
                     /translation="MNGTGRDSQNSGVWRYRQVGRRAVRLEVRGDWPGAAEAWLRAAQ
                     SAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            complement(2054..2614)
                     /locus_tag="ACHUU3_003389"
     CDS             complement(2054..2614)
                     /locus_tag="ACHUU3_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR2083377.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELTAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAERLDKIRRQNRIKAELQAVLDEQ"
     gene            complement(2717..3577)
                     /locus_tag="ACHUU3_003390"
     CDS             complement(2717..3577)
                     /locus_tag="ACHUU3_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR2083378.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLIVIAGQDSVNPPEQGKALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            complement(3636..4382)
                     /gene="traX"
                     /locus_tag="ACHUU3_003391"
     CDS             complement(3636..4382)
                     /gene="traX"
                     /locus_tag="ACHUU3_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFR2083379.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWFQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSYRLYRAEDRAERLALLACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            complement(4402..9672)
                     /gene="traI"
                     /locus_tag="ACHUU3_003392"
                     /pseudo
     CDS             complement(4402..9672)
                     /gene="traI"
                     /locus_tag="ACHUU3_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            complement(9672..>9949)
                     /locus_tag="ACHUU3_003393"
     CDS             complement(9672..>9949)
                     /locus_tag="ACHUU3_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFR2083380.1"
                     /translation="SSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISESG
                     EVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF"
BASE COUNT         2070 a         3112 c         2342 g         2425 t
ORIGIN      
        1 tactgtcgtt taatgtgatt taaactgtga aatagtatgg ttttcagtta tggaaacgcc
       61 gggagcgggg aaaacttgct ttttcccgtt tccggggttg gacaactgag caacgcgaag
      121 gcgtcagcta cgatgttccg gtgactgctg agcagcccct gttgtattca gccattctcc
      181 ggtcatctgc agcagttttc tggcatcgtg gcttctgagc aacggaatac cagcctgcct
      241 cagaacctct tccagaagaa tatcccggcg tctgcgttcc ggtcgtaaat ggctggcatc
      301 atccagctct actgctgcaa caatactgaa cgaacgccgc tcaacgataa ccacatcaac
      361 atgccactga gacaccatcc tgaataactg ccaccactgg cgtgatcgtg gccggatatt
      421 cccgttcagc tggacgatat ccgcgatccg tacctgcgga cacagatacc accgcttcat
      481 atccacaatt ctgaacagtc ctttcataaa actgacttcc ctttctgtca gcagcgtctt
      541 ctgacgttgc cagtattcac cgccggacag tattcgctct gcatcaccgg tccgtatacc
      601 tgccgcatgc aattgcatca gcaaaggaga atgctgattt acacttctga ggataatccg
      661 gataagtaac gctgcaataa tcagaacaat aagaataaaa ataatcagct tcattgttta
      721 tctgctcctg gtaattaatt atgctgaatt attaccttac atatttccat atctcttttg
      781 gtatcctgtc atataacttt ccggtgatta attccgctaa ccggtggtct gctgctgaat
      841 taaatgattc acgttcactg tttaataaac tgtttctgtt tttatgaata attttttcca
      901 gcgtattgac tgatgaacat cgccgtaact gatacagcca ttcctgtttt gttttcgcca
      961 tttttattcc cgctgagaac agatgaatga taagattgcc atcgctttat ttacggtgtc
     1021 tccatatcca gggaggaacc ggatcagcat ctgaccagag cccacgtttt tgttgtctgg
     1081 cttcgttctg aaggacaggc agcacgggat ctgtgttgta ctgctcatat acccaggcag
     1141 ccccggcccg gaccataaac tgattaacat tcattccgtc tggtgcataa acctgcccga
     1201 gaatacgacc ataacgatct cgctgaaaat atgtgacggt aaccgttttt cctgcaacca
     1261 gtgatttcat catgtccgtt gaccaccggc cataatcctg tttcttttcc ggtgcatcaa
     1321 tatttatcag cctgatccgc actgccttcc gtgagtccat gacctcgatg gtatcaccgt
     1381 ccagtacacg aacaacccgt ccatgaatat ctgcacacaa taccggccat gaaaagataa
     1441 ataataccag tggaatatat ttcctcatgt tattctccat atcagataaa tggcacctgt
     1501 atttcaggta atcctgaata tcgcagcaca cactgaataa caccttattt tgtgaaatta
     1561 atcacaaaat ataaaatcac gacttaatat ggaaaaaact ctgtgctact cagaactgtc
     1621 cggtcatact tcccgaccgg atattaaaaa aagcaaatct catgcattat tcagccttct
     1681 gaatccctgg agtaatgaag gctgcttttt cgttacaccc ttccccggca gcgccggtgg
     1741 cagtgttcag ccctttttcg ggcaaactgc tgccagtctg tccggggagc agactgcgca
     1801 gcccgtagcc atgcctcagc ggctcccggc cagtcccccc tgacttccag ccggacagcc
     1861 cggcgtccga cctgccggta gcgccatacc ccggagtttt gcgaatcacg ccctgttccg
     1921 ttcattttcc ttcccccttt aatccaggcc ggtgaatgtg ttataaattt ttataacctt
     1981 cttctgccgg gtatggaaga aggtttcaga gaagccttat ttttccgcct cctttagtgt
     2041 gaaggaggct tttttattgt tcatcaagca cggcctgaag ttctgccttt atccggttct
     2101 ggcggcggat tttatccaga cgctctgccg catacgcttc ttcctcctga gaaatatgct
     2161 ccgtcacata cccttccgtg tcataccggc aggcaccggc tttcatggca cacagatagc
     2221 tttctgaacg ggtgatggcc ttcagcgccc tgcgcagttt tttatgcgag agcgggatat
     2281 tccgctgcgc cacgtcttcc agtaacacgt cccggatacc gcaggccaga agtcggggtg
     2341 tgtcaccgtc aaataatccc ggccaccagg gtttcagggt gttcacggcc tcatccagcg
     2401 agggcaggtt cagataaatg gacagcgcct gtctggcctg cgcttttttt gctgtcagct
     2461 ctgcttcccg ggcagccttc tccgccagtt tctgcttttt caccttccat tttggtggcg
     2521 tggtgacatt gatgatggtt ttccggctcc gggtcggtgt ttccccttcc gttttccgtt
     2581 tcagtgtcag taccggtcgt ttctgctctg tcatgattgc cctgaccttt gtgaaaatat
     2641 gtcctccagg tcgttgaaac tactcactat gatattaagc tgacctcaac gacctctgac
     2701 atattaatac cgacaattaa agatgttttt taaaccattg cgtctgtact gccgccacac
     2761 gttcaaaaaa cgcgccttca taaatatcat aatggcatgc gtcagcttcc tcgtataatt
     2821 ccttagtacc agaagcaaca gcatcatata aggcttttcc ctgttctggt ggattaacac
     2881 tatcctggcc ggcaataaca atcaaaactg ggcactgtac tttggctgcg ctctctgccg
     2941 gtttatattg caacgtctcc ataaccgtaa ggaaaggtat ttttatatcc atttcaggat
     3001 actgaccttt taccttctcg aagaatacct ttgattcatt atcactcagt acccgggtaa
     3061 cccccacaaa catctctttc ccggtgtttt tcttcttctc tgccatttta ttcagcgtgg
     3121 acagaaaaga cgctctctcc gattcattca tctctccggt aaccagaaca tcaccgtcag
     3181 caaaagccag ctggctgaca atacatttta cgcgttggtc ctgcgcagct gcactgaaga
     3241 catgcccccc tcccagcgat gttccccata acccaatacg ctgattatca atacattcct
     3301 gcttttctgc ccaattgata actgaaataa tatcttcggt ctgcattgca ggaaccagac
     3361 gccctcgttc tccatcactc tcaccgaatc ctcgataatc aaaagtaatc gttgcgaatc
     3421 ctgcttctgt gaaggcattc gcaaagcaag ggagcagaac attcctgata ccgcaaaacc
     3481 catggcataa gataatcaaa ggatgtttta tatttccttc aggaacacga aatgtcaggg
     3541 caataccttc agagagtttg tgatccgtaa ttttcataaa tatcctgtgt tatgttgttt
     3601 attaataatc agaatcagcg tcagtatcat accagtcaca acgccagaac gcccagcaca
     3661 gccagatgac aggcgtagaa taccgggaaa aaatccccgg gccagaaacg gggtaatgat
     3721 tttcctgcac acgacaccag accaacggtc agcaccgtca ttacaagacc ggccaccgcc
     3781 gctgcatcac tggaggcaag gttaagtgcc ggaataacgg ccagcaggca tgcaagcagc
     3841 gccagacgtt ccgctctgtc ttccgcccgg tacagtcggt aactgaccgc cagcatcagc
     3901 agcccggcaa tgccgtaact ggtgccggac aagggccccc acagggccat cagaagaatg
     3961 gccgctgctg tacgccaccc actgcgcgtt tcacaccacg tcagcacctg tgccgccact
     4021 gcaaaggcaa acaggatatt cccctcatac cagggaaagc ctgccagata atatgcgaac
     4081 tgggcaataa ttccccatcc ccacagccgg ttaatggccg gttgccggat atgcgcatga
     4141 cgggacagat tcagccccca caccagggca aacagcggaa aggctcctcg cccggccaga
     4201 aacatccatt cctgcttcag ctggaatatc agattgatgt ggtccagtac catcagcacc
     4261 agtgccaccg ttttgatgat atcccgctgt ccgggtgacc agaccagaaa tgactgaaac
     4321 caggtatcgg tccgtgcagc cagtgaatcg ttacgtgtcg tgttcgtgtt atccgttgtc
     4381 atcagtgttt atcttcctgt atcagtctcc gcccagggtt ttctctttct gcaggtcacg
     4441 gaccatatcc cgctccatct gctgcagacg ctcctgcagc agattttccc gggcaacctc
     4501 ccggaccgct tcccgctccc gttgtgattc acgcagcaca ctctccggca gcgcggcttc
     4561 ccgttcagtt atggcagacc ggtcacgctc ctgtccggca atctccctga cagcctgctc
     4621 tgttttgccg tccggcaggt caggtttgtt ctctgccatt ttacggacaa tttcatctgc
     4681 gcggcgttcc gtttcacggc ggatggcctc ttcagcctgc cgctgtgcca gcacttcagc
     4741 tgtaaccggt tcaccatttc cggcccccgg ctggacgctg ttgtccggga tatcccccca
     4801 cacgcgacca ccggtgatgg caccgggatt ccacggacga ccttcaccgg caattcttac
     4861 caccacaccg ctgtcgggat tatcacgggc aatccggaca ccctcctgca tattatcagc
     4921 cagcaggctt tctccgttac ggctgccctg cagggccacg aactgcgcat ccccgctgcc
     4981 ttttacccga ccttcaccgc tgaatccccg cagcccgttt ccgtcatccg tggtcagcgg
     5041 gttcagccag ataccggcgg acctgccgtt acggtcaaac gccggcagtg ccacatacgg
     5101 ttgcggatat ttacgtcccg gagcaataaa ccgtgcagga ctgtctcccc cggccagccc
     5161 tgcctgacgg agaacggcac ggcctgccgc cacgtcccgc agttcccgtg ccgtactgaa
     5221 cagccgctgc gcattcatga cctcccggtc cggtttcggc tccagcacat cgtgggcagt
     5281 tcctttctgt acggcattgt taatggcatc cgtccagccc tgacggttat cggtgtacac
     5341 ctgcacatgc tgcttcatac gcgacagggc cacgtaggct gactcaaagc cggccatctg
     5401 tttccggtta ccttccgtgc cttcaagcgc aatggcaaag gtttcacttg ccccctgcgc
     5461 accgtgggcg gtgatggcat aggccaggtc aatatgttgc tctgcccgct cctgaccggg
     5521 gcgaatcacc cgggtctgct gtccgtccga cagcgtgaca ctgtcaccgg aaaccgctgt
     5581 caccgtccag acgctgttgg ccacataacc gcgctcccgg tcactcttcg tgaagcgcat
     5641 ccggtcaccg gttcccaccc ggatggtgtc cggagtgtac agtgtgacgc cttcagccac
     5701 cgcctcccgg ggcgaaatca gccgcgtgtt accttccgca tcctgcaggg ttatcagccc
     5761 gtcatccttg ctgataccgg caatccggtg atacacatta tccaccaggg caagtgcgtc
     5821 ccgatgtgtc tcccaggtgg agagacgacg caactccccg tcgcgtatat tcgctgtgtt
     5881 caggacaggc accatgacct gctcctgccc cagctcaccg gccttttccc gtgcatcatg
     5941 aatcatgctg ttcagcacgc gccggtcctc attcaggtgc gtgacaataa gcgtctgctc
     6001 ccgtgctttc ggtgtcctgc cggtatagtc gcgaacaatg gcttcataca gtgtcatggg
     6061 gacatccggg aaagcctcgc ctttcagcat cgacttctgc tgcgcttctg ccagtttcgc
     6121 ttcctgtctg tgactgaact ccgtcacgga gtgctccggt gcccatgcgc cctccagacg
     6181 tggcacctga gacggtttca cactctcaag cccggacagt gccctttcca catcccggtt
     6241 aatcaggctg tataccgcct cccgcagttc cggcgtctga cgcacaatct ccttcatgat
     6301 ggccacatcg gcagcactgc gcgtctgctg gagacggaaa ggctgaccgg gcgcgatggc
     6361 ctgcagctgg tccgtgtcac cgctggccac agcacgaccg ccaccggccg caatcagggc
     6421 gtatgcccgt gccatgtcgg tattgcccac cattgagctc tcatcgagca gaaacagcgt
     6481 gttgctgaaa tccggcgttt ctccgctgcg ctgctgcagc tgcgtgtcat gcagaaagga
     6541 cgccagtgtc tgtgcatcca cgccggcgct gcgcatctca ccgaccgcac ggtgcgtggg
     6601 ccccagcccc acaacccggg gacgctcact ctccggcagc atgttcacgg ctgacatcac
     6661 cgcccggaac tgagtggtct tacccacacc ggcatacccc tgtaccaccg tgaaacggtc
     6721 ggacgtttcc agtatcatac gggtggcggc acgctgtccc gacgttaacg tctccatgag
     6781 ttcgccgggt actctctcca tcagcggcgt gaccgcctcc ttgccttcga gaatatggcg
     6841 aagaatgctc ttttctgcct catacgacgc acgggaaacc agcaggcctg tgccatagcc
     6901 ttttgccaca tccacataca gtaagtcacc gcgttttatc tgcgcattga tttcccctcc
     6961 cagttcagta aaaccggttc cttctgcagc aaacgacttc gcctctgtca gcaggtccac
     7021 catgctgaag gccaggtttt tactttccag cacaggaagg gccagatgaa tggcctgctg
     7081 tgccggcgtg tgaagcccgg ctttctgcag actgatagcg gtttccagca atgtctcacc
     7141 ggcacgcgcc tttatctgct cagaaaccac cgtaaaggac ggatggcggg cgagtttttc
     7201 cgcagtacgg gtttcatcca gtgaggaata cagccggaca tcacgaccgc tgcgggccag
     7261 accgttcaga gtggcgttgt ccattgccat ctgtgtgacg gaggcaaaca ccgtcgcact
     7321 gtcactgacg gaatgcccgg gcgtttccac ccagccgttc tccagcttca gtgccgtgaa
     7381 cggtgaatca gccacaggca gggtggccgg ttcagcccgc cccggcacaa caaccgtcat
     7441 cgcatcttca ctgacggatg ccacctgcag gcggtcaccg ccggagacgc gaagtccggg
     7501 aattttccct gtcaccctca gtcgctcgcc gtctgccacc ggcatttttt ccggccggaa
     7561 cagcgaccag ctgctgtcca gggaggaaat acgcaccacc tgcgtttcgc cctgcgcatc
     7621 ccgcagggtc aggctgtgac tctgcgccgt cacccggtcg ataacatagc ggtcatgact
     7681 gcgtgtctcc gggttccact gctccatcac catccccggt cggtacatat cccgcagata
     7741 acggctccgg ctgtccagcc agaccggtga cagggcggtc atggtcacct ccgggtgtcc
     7801 gagcacgccc tgtgttttca gctcactgcg aatggcctgt gtcagtatag cctgttcccg
     7861 taccccgctg acctgtgcca cgctctcttc tccggctttc acgctggccg caaaatctcc
     7921 ggccagccgg tcatagcgga cattccggtc cggttcactg atgatggttg ccggtcgctg
     7981 ttctccgccc tgccagcgat acgtgttcac cccggcatcc ttcatggcca tcagcgcact
     8041 gcctgtaccg gttcgctgcc cgctgtcggt tatcaggacc tgtacgttat gacgtgcggc
     8101 accgtccagc agggttaacg tctctttcag ggagagtttt tcgccctggt caacgataac
     8161 ggtactgccc ggtgtgaagg ccataccctc cagcagctga cgacgtccgg ttatcagctc
     8221 accggacagc cgttcatcct gcttcaggtt catctgcgag cgacggtcag cagcgataat
     8281 ctgcacctcc cgcccctgct cccgggccat catgaccagt tcagccaccc gctcacgctg
     8341 cccggctgca ccgccctgcc cggacacaat ggccagcgac ggacggtcct gtgccagaac
     8401 actgaccgca tcgctgtaac cagccgtccg ggggacactt ttctccggat gtacggtcac
     8461 ccggttctgt ttcatgatgt cacgactgag tgcccggacg gacagctcat cgagcacatg
     8521 aatcccggat gtgaacagcc ccttctcacg gtcgaggggg ataagctgct cacggctgat
     8581 ggcctcatcg ataccggcgc gtgcccgttc aatcacacca ttttccggcg gcagtatgcc
     8641 gaccgtcctg gccagcacgt ccgtatacgt gaactgcact tttcgttcac ttaatccggc
     8701 aatcgcctgt gtcaccgcct gctgcacatc cggcccgtcc tgtgaagcag gcccgggcgc
     8761 ctgcgtgcgg atctccgcac gctgatccgc cgcgtcacga tatgcccgga tgtcgaaccc
     8821 ggtttccttc agcgtctgca tccattcagc cattctgatt tccggatcga cgtgctgttt
     8881 ggattttcgc gtatccagtg ccgccacatc ccgggatttc agcgaggcgt cttcaccgac
     8941 ggcctccctg atagcctgtg aacgactgga aaaggcctcc accggtacgc ccggcatctc
     9001 ccacataccg tgcttaccga ccacttcggt ttcataaccc agcgcctcaa cgcgctgacg
     9061 taactcactc taatatattt tcccgaacgc aatccggttc gctagcacgt tctcggaaaa
     9121 tccggttttc cccactttgt cactgctcag tgtcttccac tcgccgttat gctgcgtgac
     9181 attagccacc actacatgcg tgtgtaactg tgggtcctga tcgcgactgg tgtcatggtt
     9241 aaacagtgcc atcaccagat taccggtcag caccgtttct gactgtccgt ccgtcatcac
     9301 ccgtgtggaa gccagcgcct ccacctgacg gacagcaaaa tccacggcct ggttatgtgc
     9361 atcaataaga cgtttatccc ccccgagcat ggccatcatg gagacacttt tgggggcgga
     9421 gaaggtcagg tcgtagccgg gacgatgctt attactgcca tcctgcatgc ggcttaaatc
     9481 cgccccgtcc ggcagcctgc cctccagaag acgggtaaaa acatccttat cgacgctgcc
     9541 ctgcagccca agctgttcag ccccctgacc agcccagcgt tctcccatgc tgcccagcac
     9601 atagtaatta tccttgtcgg tgtaatagtt tccggcactg cctgccgatt taacaacgct
     9661 gaaactcaac atcagaaatc atctcccggc tcaacatcct ccccgcgctc ccggtgcaca
     9721 ttaatgttca cctcttcacg acgctgcata tgctgctgga tgtccggatg attttcctgt
     9781 tgccatgcct cataagcggc catatccacc acttcaccgg attcactgat cccgggtggc
     9841 agttgctgtt ccatctcttc ttccggtttc attttcagct cctgctcgat cccccctgcc
     9901 ggaacactca cacctgcatc tgacttctta tcattgatga cagaagaca
//
LOCUS       DAZPLI010000143         9739 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZPLI010000143 DAZPLI010000000
VERSION     DAZPLI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.144"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            39..7104
                     /gene="yeeJ"
                     /locus_tag="ACHUU3_003394"
                     /pseudo
     CDS             39..7104
                     /gene="yeeJ"
                     /locus_tag="ACHUU3_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
     gene            complement(7366..8418)
                     /locus_tag="ACHUU3_003395"
     CDS             complement(7366..8418)
                     /locus_tag="ACHUU3_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFR2083381.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            8733..>9739
                     /gene="shiA"
                     /locus_tag="ACHUU3_003396"
     CDS             8733..>9739
                     /gene="shiA"
                     /locus_tag="ACHUU3_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFR2083382.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
BASE COUNT         2682 a         2359 c         2341 g         2357 t
ORIGIN      
        1 agggcgcatt tatgttagct cgttcaggga aggtaagcat ggctacgaag aagagaagtg
       61 gagaagaaat aaatgaccga caaatattat gcgggatggg aattaaacta cgccgcttaa
      121 ctgcgggtat ctgtctgata actcaacttg cgttccctat ggctgcggca gcacaaggtg
      181 tggtaaacgc cgcaacccaa caaccagttc ctgcacaaat tgccattgca aatgccaata
      241 cggtgcccta cacccttgga gcgctggaat cggcccaaag cgttgccgaa cgtttcggta
      301 tttcggtggc tgagttacgc aaactcaacc agtttcgtac gtttgctcga ggttttgata
      361 atgtccgcca gggtgatgaa ctggatgtcc cggcacaagt tagtgaaaaa aaattaaccc
      421 cgccgccggg taatagcagt gacaacctcg agcaacagat agccagtact tcacagcaaa
      481 tcgggtctct gctcgccgaa gatatgaaca gcgagcaagc ggcaaatatg gcgcgtggat
      541 gggcctcttc tcaggcttca ggcgcaatga cagactggtt aagccgcttc ggtaccgcaa
      601 gaatcacgct gggcgtggat gaagatttta gcctgaagaa ctcccagttc gattttctcc
      661 atccgtggta tgaaacgcct gataatctct ttttcagtca gcatactctc catcgtactg
      721 acgagcgtac gcagattaac aacggcttag gttggcgtca tttcactccc acatggatgt
      781 cgggcatcaa cttctttttc gaccacgatc ttagccgtta ccactcccgc gccggcattg
      841 gcgcggagta ctggcgcgac tatctaaaat taagcagtaa cggctatttg cgactgacca
      901 actggcgcag cgcacctgaa ctggacaacg attataaagc acgcccggcc aatggctggg
      961 atgtacgcgc agaaagctgg ctacccgcct ggccgcacct tggcggtaaa ctggtctatg
     1021 aacagtatta tggcgatgaa gtggccctgt tcgataaaga cgatcggcaa agtaatcctc
     1081 atgccataac cgctggactt aactataccc ccttcccgct gatgaccttc agcgcggagc
     1141 aacgccaggg taaacagggc gaaaatgaca cccgttttgc cgtcgatttt acctggcaac
     1201 ctggcagcgc aatgcagaaa cagcttgacc cgaatgaagt cgctgcacgg cgtagccttg
     1261 caggcagccg ttatgatctg gtggatcgca acaacaatat cgttctggaa tatcgcaaaa
     1321 aagaactggt tcgcctaacc ctgacagacc ccgtgacagg gaagtcagga gaagtgaaat
     1381 cactggtttc gtcgctacaa accaaatatg ccctgaaagg ctataacgtc gaagccaccg
     1441 cactggaagc tgccggtggc aaagtggtca caacgggtaa agatattctg gttaccctgc
     1501 cggcttaccg gttcaccagt acgccagaaa ccgataacac ctggccgatt gaagtcaccg
     1561 ccgaagatgt caaaggcaat ttgtcgaatc gtgaacagag catggtggtc gttcaggcac
     1621 ctacgctaag ccagaaagat tcctcggtat cgttaagtac ccaaacattg aacgcggatt
     1681 cccattcaac cgccacactg acttttattg cgcatgatgc agcaggtaat cctgttgtcg
     1741 ggctggtgct ctcgacgcgt cacgaaggtg ttcaggacat caccctttct gactggaaag
     1801 ataatggtga cggaagctat acccagatcc tgaccacagg agcgatgtct ggcacgctga
     1861 cgctgatgcc acagctgaat ggtgtggatg cggctaaagc ccccgccgtg gtgaatatca
     1921 tttctgtttc gtcatcccga actcactcgt caattaagat tgataaggac cgttatctct
     1981 ccggcaatcc tatcgaggtg acggtagaac tgagagatga aaatgacaaa cctgttaagg
     2041 aacaaaaaca gcaactgaat aacgcagtca gcatcgacaa cgtgaaacca ggagtcacta
     2101 cagactggaa agaaaccgca gatggcgtct ataaggcgac ctataccgcc tataccaaag
     2161 gcagtggact tactgcgaag ctattggaat gaagatttgc ataccgctgg atttatcatc
     2221 gacgccaacc cgcagtcagc gaaaattgcg acattatctg ccagcaataa tggtgtactc
     2281 gccaatgaga atgcagcaaa caccgtctcg gtcaatgtcg ctgatgaagg aagcaaccca
     2341 atcaatgatc ataccgtcac gtttgcggta ttaagcggat cggcaacttc cttcaacaat
     2401 caaaacaccg caaaaacgga tgttaatggt ctggcgactt ttgatctgaa aagtagtaag
     2461 caggaagaca acacggttga agtcaccctt gaaaatggcg tgaaacaaac gttaatcgtc
     2521 agttttgtcg gcgactcgag taccgcgcag gttgatctgc agaagtcgaa aaatgaagtg
     2581 gttgctgacg gcaatgacag cgccacaatg accgcgaccg tccgggatgc aaaaggcaac
     2641 ctgctcaatg acgtcatggt cactttcaat gttaattcag cagaggcgaa actgagccaa
     2701 accgaagtga atagccacga cgggatcgcc acagctacgc tgaccagttt gaaaaatggt
     2761 gattataggg ttacggcctc tgtgagctct ggttcccagg ctaatcaaca ggtgaatttt
     2821 atcggtgatc aaagtactgc tgccctgacc ctcagtgtgc cttcaggtga tatcaccgtc
     2881 accaacacag ctccgcaaca tatgactgca accttgcagg ataaaaatgg caacccacta
     2941 aaagataaag aaatcacctt ctctgtgcca aacgacgtcg caagtaagtt ctcgattagc
     3001 aacggaggaa aaggcatgac ggatagtaac ggggttgcaa tcgcctccct gaccggcacg
     3061 ttagcgggca cgcatatgat catggctcgt ctggctaaca gcaatgtcag cgatgcacag
     3121 ccaatgacgt ttgtggcgga taaagacaga gcggttgtcg ttttgcaaac atcgaaagcg
     3181 gaaatcattg ggaatggcgt ggatgagaca actctgacag caacagtgaa agatccgtcg
     3241 aatcatccgg tggcggggat aacggtaaac ttcaccatgc cacaggacgt tgcggcaaac
     3301 tttacccttg aaaataacgg tattgccatc actcaggcca atggggaagc gcatgtcacg
     3361 ctgaaaggta aaaaagcggg cacgcatacg gttaccgcaa cgctgggtaa taacaatacc
     3421 agtgattcgc agccggtaac atttgtggcg gacaaagcct cggctcaggt tgtcctgcag
     3481 atatcaaaag atgagatcac aggtaatggc gtcgatagcg caacgctaac tgcaacggtt
     3541 aaagatcagt tcgacaatga ggtgaataat cttccggtaa cattcagctc agcctcttca
     3601 ggactcaccc tgaccccggg agtaagtaat accaatgagt ctggcatcgc gcaggccact
     3661 ctcgcaggcg ttgcctttgg tgagcagacg gtcactgcat cactggctaa taatggtgcc
     3721 agcgacaaca aaactgtgca ttttattggc gacacagcgg cggcaaaaat tatcgagttg
     3781 acgcctgtcc cagacagcat aatcgccggt accccgcaga acagctccgg cagcgccatc
     3841 accgccacag tcgttgataa taatggcttt ccggtgaaag gtgtgactgt gaacttcacc
     3901 agcagaacaa actctgccga aatgacgaat ggcggccaag ccgtaacgaa cgaacagggt
     3961 aaggctaccg tcacttatac caatacccgc tcctcgatag aatcaggagc gagaccggat
     4021 accgttgagg ccagtctgga aaatggtagc tccacgctta gcacatcaat taatgtcaac
     4081 gctgatgcgt ctacggcaca tctcacctgg ctacaggcac tttttgatac agtctccgca
     4141 ggcgagacaa ccagtctgta tattgaggtg aaggataatt acggcaacgg tgtcccccag
     4201 catcaggtga ccctcagcgt ttcaccaagt gaaggcgtga cccccagtaa taacgctata
     4261 tatactacca accacgacgg caatttttac gcaagcttta ccgctacaaa agccggggtt
     4321 tatcaagtga cggcaaccct cgaaaatggc gattcgatgc aacaaacagt gacctatgtg
     4381 ccgaacgtca cgaatgccga aatcacgctg gcaacctcga aggatccggt gattgccgac
     4441 aataacgatc tcacgacact aacagcaaca gtcgctgata cagagggcaa tgcgatagcc
     4501 aacactgaag taacatttac tctgccggaa gatgttaagg cgaacttcac gctgagcgat
     4561 ggcggtaaag cgattactga tgctgaaggc aaagcgaaag tcacgctgaa aggtacaaaa
     4621 gcaggcgctc atactgttac agcatcgata actggcggta agagtgagca gttggtggtg
     4681 aactttactg cggatacgct cactgcgcag gttaatctta acgttaccga ggacaatttt
     4741 atcgctaata acgtcgggat gaccaggctg caggcaacag tgactgatgg aaacggcaac
     4801 ccgttagcca atgaggcggt gacattcacg ctaccggcag atgtgagcgc aagctttact
     4861 ctcggacaag gcggttccgc cattactgac atcaacggca aggctgaagt tacactgagc
     4921 ggtacaaaat ccggcaccta ccccgtgaca gttagcgtga acaattatgg tgtcagtgat
     4981 acgaaacagg tgactttgat tgccgatgct ggtaccgcaa cactagcctc cttaacctct
     5041 gtatactcat tcgtcgtcag cacgaccgag ggcgcgacca tgactgcaag cgtcactgac
     5101 gctaacggca acccggtaga aggcataaaa gttaatttcc gcggaacctc cgtcacgcta
     5161 agcagcacca gcgttgaaac ggatgatcgg ggtttcactg aaattcttgt gacaagcacc
     5221 gaagtcggac tgaaaacagt ttcagcctct ctggcagata aacctactga agtcatatcg
     5281 cgattactga atgcaaaagc agatattaat tctgcaacga ttaccagtct ggagatacct
     5341 gaaggtcagg tcatggtcgc acaagacgta gcagttaaag ctcacgtcaa cgaccagttt
     5401 ggcaacccga ttcttaatga atctgtaaca ttcagtgcag agccaccaga gcacatgacc
     5461 atcagccaaa atattgtctc tactgatacg catggtatag ccgaggtctc catgacgccc
     5521 gaaagaaacg gttcgtatat ggtgaaagca tccctggcga atggagcctc acttgagaaa
     5581 caactggagg ctattgatga aaaactgaca ctctcggcgt ccagcccgct tatcggtgtc
     5641 aattccccaa caggtgcaac tctgacggca acgctaactt ctgcaaatgg cactccagtg
     5701 gagggtcagg tcatcaactt tagcgtaacg ccagaaggtg cgacgttaag tggcggaaaa
     5761 gtgagaacta actcttctgg tcaggctcca gtcgttctga ccagcaataa agtcggtaca
     5821 tatacggtga ctgcatcgtt ccataacggc gtaacaatac agacacagac aaccgtgaaa
     5881 gtcactggca actcaagcac cgcccatgtt actagcttta tcgctgatcc atcgactatc
     5941 gccgccacca acagtgattt aagtacctta aaggcaacgg ttgaggatgg cagtggtaac
     6001 ctgatcgaag gtctcactgt gtactttgcc ttaaaaagcg gctctgccac attaacgtca
     6061 ttaacagcgg tgaccgatca aaacggaatc gcgacaacaa gcgtgaaagg agcgatgaca
     6121 ggtagcgtca cggtaagcgc agtcacgacc gctggtggaa tgcaaacagt agatataacg
     6181 ctggtggcag gcccagcaga cgcctcaaag tcagtcctta agaacaatcg gtcatcattg
     6241 aaaggagact ttaccgatag tgctgagcta catcttgttc tgcacgatat atcaggcaat
     6301 ccgatcaaag tttctgaagg gctggaattt gtacagtcag gtaccaacgt gccctatgtg
     6361 caagttagtg caattgacta cagtaaaaat ttctcaggcg agtacaaagc cactgttaca
     6421 ggaggcggag agggtatcac aacgctgatc cctgtattga atggtgttca tcaagctggt
     6481 ctgagtacca caatacaatt cactcgcgca gaagacaaaa taatgagcgg tacagtatca
     6541 gtcaatggta ctgacctacc gacaactaca ttcccttcgc aggggttcac cggggcgtat
     6601 tatcagttga ataatgacaa ctttgcccca ggaaaaacgg cggctgatta tgagttttca
     6661 agctctgcct cctgggtcga tgttgatgct accggtaaag tgacatttaa aaatgtcggc
     6721 agcaattcgg aaaggattac ggcgacacca aaatcaggag gccctagcta tgtatacgaa
     6781 atccgtgtga agagttggtg ggtgaacgcc ggcgaggctt tcatgatata cagccttgct
     6841 gaaaattttt gcagcagcaa tggctacacg ctccccagag caaactattt aaaccacagt
     6901 agttcccgag gcatcgggtc actgtacagt gaatggggag atatggggca ttacacgact
     6961 gaagctggtt ttcaatcaaa tatgtattgg tcatctagtc ccgcaaactc aagcgaacaa
     7021 tacgtagttt ccctggcaac aggtgatcaa agcgtatttg aaaagcttgg gtttgcttat
     7081 gcgacatgtt ataaaaacct ctgattttcc gttataattt cttaaagagt gctccattcg
     7141 gagctctctt ttttacattc cctctataga gcttgccctt ctctcacaga aaatgaataa
     7201 ataaaaatgc ggcaccgcca gaattgcgtt cgatgtgact atgatcgtat ccggtgagta
     7261 ctgctttgat tcaaaggctg gttttctgtg atgattgacc agcctcagag tattgatgac
     7321 accaccgttc ataacagatc ggcattacta tgcataagta ctgtctcata ttctcgccaa
     7381 aacacttttt aatgcctttg cgaattcatc cagacttata ttgtcaattt tttgcccaac
     7441 gacgactgac tccgagacat cggccattgc gggccatcgc gttttgtaat cagcataaat
     7501 tgcaacaaca ggggtattat aggcacttgc catatgtaat attgaggtgt caggagttat
     7561 tgcgatataa gcatctttta tgattgctgc tgaacgttta atcgagggtg gtaaggataa
     7621 acggtagaca ttattataac aatccactaa tgccctggct ttatcttcac ctttgggtcc
     7681 atgaacgatc actattggta tatctgtttc actttgaatt tttgctatta gattttctgc
     7741 aatcgataat gaaaatgtac gttcctgcga gctaccttct aaattgaacg caatgtaaga
     7801 tcctaacgat cgcatttcct cgcgcacctc atcgagtaca tcctcactta gtggcaattc
     7861 atatattggc ctgactgctg gaaaacccgc ctcacgcatc ataaatgccc atgtcatagg
     7921 tacgggagcc cggagtttct gatccatacg ggacgcgttc ttgcacaaag gggagtagca
     7981 attcatggtt ataccaacaa cctgaaaatt cgtttttgct ttcaactgac tgataaataa
     8041 catcgttttc aggttctttt tacgcatccc ctcaatacaa agatccggcg taccgtattg
     8101 ctgtgttatg ttctttgcta aatcttttat ttcttttaat gttgcgtgat cctgcatagt
     8161 cattgtgact aatgttaatt tagtctgttc aagtttaagc gcattaaaga cttctaaatt
     8221 aattgtcgac gttacaatta aaagatgctt aattttatgc aattcaagcg cccgaataac
     8281 aggaaagatg gccatagcat cgccaatctg atcgggaata tggatgacaa caaagtctgt
     8341 tttttcaata ttgaaattat aagctttata atcgtagtaa ctaaatgcaa tacgtctcaa
     8401 caatgatgct aaaaacatac ctaacctcgc ctccctactg gttataatgc aatgcagtct
     8461 atcagactca tcagggtgcc attttgtgca tatgcggact tttatgtttc atatctctaa
     8521 cctgtgggtc ctctgcttaa tccttaaaca acaccagcaa ctcctgcgct ttcatcttcc
     8581 atcgaatttt tcatgttgcc gctaatcagc cataaaatca tttgcagatg cgctctgtcg
     8641 aggtagtctc ataaggttcg tttatagatc gacggcaatg tgagttacct tttccatact
     8701 aattataaaa agacagtaca aacaggatca ttatggactc cacgctcatc tccactcgtc
     8761 ccgatgaagg gacgctttcg ttaagtcgcg cccgacgagc tgcgttaggc agcttcgctg
     8821 gtgccgtcgt cgactggtat gattttttac tctatggcat caccgccgca ctggtgttta
     8881 atcgcgagtt tttcccgcaa gtaagcccgg cgatgggaac gctcgccgca tttgctacct
     8941 ttggcgtcgg attccttttc cgtccgctcg gcggtgtcat tttcggtcac tttggcgacc
     9001 gactgggacg taagcgcatg ttaatgctga ccgtctggat gatgggcatc gcgacagcct
     9061 tgattggtat tcttccttca ttctcgacca ttgggtggtg ggcacctatt ttgctggtga
     9121 cactgcgtgt cattcaggga tttgccgtcg gcggcgaatg gggaggcgcg gcgttgcttt
     9181 ccgttgaaag tgcaccgaaa aataaaaaag ccttttacag tagcggtgta caagttggct
     9241 acggtgtagg tttactgctt tcaaccggac tggtttcatt gatcagtatg atgacgactg
     9301 acgaacagtt tttaagctgg ggctggcgca ttcctttcct gtttagcatc gtactggtac
     9361 tgggagcatt gtgggtgcgc aatggcatgg aggagtccgc ggaatttgaa caacagcaac
     9421 attatcaagc tgccgcgaaa aaacgcatcc cggttatcga agcgctgtta cgacatcccg
     9481 gtgctttcct gaagattatt gcgctacgac tgtgcgaatt gctgacgatg tacatcgtta
     9541 ctgcctttgc acttaattat tcaacccaga atatggggct accgcgcgaa cttttcctta
     9601 atattggttt gctggtaggt ggattaagct gcctgacaat tccctgtttt gcctggcttg
     9661 ccgatcgttt tggtcgccgt agggtttata tcacaggtgc gttaatcgga acgttgagcg
     9721 catttccttt ctttatggc
//
LOCUS       DAZPLI010000144         9737 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZPLI010000144 DAZPLI010000000
VERSION     DAZPLI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9737
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.145"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..56)
                     /locus_tag="ACHUU3_003397"
     CDS             complement(<1..56)
                     /locus_tag="ACHUU3_003397"
                     /inference="COORDINATES: protein motif:HMM:NF015750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR2083383.1"
                     /translation="MASVSISCPSCSATDGVVR"
     gene            114..2315
                     /locus_tag="ACHUU3_003398"
                     /pseudo
     CDS             114..2315
                     /locus_tag="ACHUU3_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            2308..3444
                     /locus_tag="ACHUU3_003399"
     CDS             2308..3444
                     /locus_tag="ACHUU3_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR2083384.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3441..5441
                     /locus_tag="ACHUU3_003400"
     CDS             3441..5441
                     /locus_tag="ACHUU3_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFR2083385.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5566..6027
                     /locus_tag="ACHUU3_003401"
     CDS             5566..6027
                     /locus_tag="ACHUU3_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR2083386.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(6068..6538)
                     /locus_tag="ACHUU3_003402"
     CDS             complement(6068..6538)
                     /locus_tag="ACHUU3_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR2083387.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6585..7304)
                     /gene="btsR"
                     /locus_tag="ACHUU3_003403"
                     /gene_synonym="yehT"
     CDS             complement(6585..7304)
                     /gene="btsR"
                     /locus_tag="ACHUU3_003403"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR2083388.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7301..8986)
                     /gene="btsS"
                     /locus_tag="ACHUU3_003404"
     CDS             complement(7301..8986)
                     /gene="btsS"
                     /locus_tag="ACHUU3_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR2083389.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9208..9273
                     /locus_tag="ACHUU3_003405"
                     /pseudo
     CDS             9208..9273
                     /locus_tag="ACHUU3_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9501..>9737
                     /locus_tag="ACHUU3_003406"
     CDS             9501..>9737
                     /locus_tag="ACHUU3_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR2083390.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEW"
BASE COUNT         2395 a         2566 c         2493 g         2283 t
ORIGIN      
        1 cgcaccaccc cgtcagtagc tgaacaggag ggacagctga tagaaacaga agccactgga
       61 gcacctcaaa aacaccatca tacactaaat cagtaagttg gcagcatcac cgccagcggc
      121 cacgatacgt attgattgaa ggcccggctg attttaatga ccgggtagac gaactgtttt
      181 tagcccacca gcttccggta gctatttaca gttattgcca gtatcaggac ggtgcagccc
      241 ccgggcgtgg tgcctggacg ccatttgctg aattttcgcc ggagtggcag gcgctacaag
      301 ccgcacgtcg tattcaggca caaacttact tcatcgattt gccttgctgg gcgcagagtg
      361 aagaagtgga cgattcgcct gatacgcaag aggaaagcca ggccttgctg ctgcgtgcca
      421 cccgcatgga taacagcgac accctgtggg atcacttgtt cgaagatgaa agccagcaaa
      481 ctgcattacc ctctgcactg gcgcactatt ttgcccaact gcggggcgat tcccccggcg
      541 atgcactcaa tcgtcagcgc gaagccttta tggcccgctg gattggatgg gcgatgcagc
      601 aaaacaatgg cgacgtgtta gtcgtctgcg gtggctggca cgctcctgca ctggcaaaaa
      661 tgtgacgcga atgcccgcag gacattaaca cgccggaatt gccctcgctg gcagatgccg
      721 ttacaggttg ttatctcacc ccctacagtg aaaagcgcct tgatgtgctg gcaggatacc
      781 tttccggtat gcctgccccg gtctggcaaa gctggtactg gcagtggggc ttacagcagg
      841 cgggagaaca actgctaaaa acggttctta cccgtttgcg ccagcacaaa ttgcctgctt
      901 cgaccgcgga tatggctgcc gctcatctgc atgcgatggc gctggcacag ttgcgcggac
      961 ataaactacc gttacgcact gactggctgg atgccatagc aggctcgctg attaaagaag
     1021 ccctgaacgc gccgttgccg tggagctatc gcggagttat tcatcccgac accgatccga
     1081 ttctgctaac gttgatagac acattagcgg gtgacggatt cggtaaactt gccccttcta
     1141 cgccacaacc gcctctgcca aaagatgtca cctgcgaact ggaacgtacc gcaatctctc
     1201 ttccggcgga gcttacctta aatcgcttta accccaatgg gctagcgcaa agtcaggtgt
     1261 tacatcggct ggcaatactg gagatcccag ggattgtacg ccagcaggga agcacactga
     1321 cacttgcagg caacggtgaa gaacgctgga aattaacccg cccgcttagc cagcatgcgg
     1381 cattgattga ggccgcctgc tttggtgcca cactccagga agccgcacgc cataaattag
     1441 aagccgatat gctggacgcg ggtggaatcg gcagtatcac cacatgtctt agccaggcgg
     1501 cgttagcggg tctggcgtcc ttcagtcaac aattactgga gcaactcaca ttattaatcg
     1561 cccaggaaaa tcaatttgcc gaaatgggcc aggcgctgga agtgctatat gccttatggc
     1621 ggctggatga aattagcggt atgcaaggcg cgcagatatt acaaacgacg ttatgcgcgg
     1681 ctatcgatcg cacgctgtgg ctgtgtgaat ctaacggcag accagatgaa aaggagtttc
     1741 acgctcacct gcatagctgg caggcgcttt gccatatcct gcgcgatcta catagcggcg
     1801 ttaatttacc cggcgttttt ctttctgcgg cggtagcctt actggagcgc cgcagtcagg
     1861 cgattcatgt cccggcgctg gatcgcggcg cggctcttgg cgcactgatg cgtctggaac
     1921 atcccaacgc cagtgccgaa gcggcgctga cgatgctggc gcagttatcc ccggcacaat
     1981 ccggcgaggc gctgcacggt ttgctggcgc tcgcccgtca tcaactggcc tgccaaccag
     2041 catttatcgc cggtttcagt agtcatttaa atcaactgag tgacgacgat tttattaacg
     2101 ccctgcccga tttgcgcgcg gcgatggcct ggctaccgcc acgagaacgc gggacactgg
     2161 cgcatcaggt gcttgagcat taccagcttg cgcagcttcc tgtttcagcg ctgcaaatgc
     2221 cgttgcattg tcctccgcaa gccattgcac atcatcaaca actcgaacag caggcactgg
     2281 catcgctgca aaactgggga gttttccatg tctgaactga acgatcttct gaccacccgt
     2341 gagctacaac gctggcgatt aattcttggc gaagcggcag aaacgacgct ttgtgggctg
     2401 gatgacaacg cccggcagat agaccacgcg ctggaatggc tgtatgggcg cgatcctgaa
     2461 cggctccagc gtggtgaacg ctccggtgga ttaggtggct caaatctcac cactcctgag
     2521 tggatcaaca gtattcacac gctgtttccg caacaggtga ttgagcggct ggaaagcgat
     2581 gccgtactgc gctacggcat tgaagatgtg gtgacgaatc tcgacatgct ggaacgtatg
     2641 cagccttctg aaagcctgct acgcgccgtt ttgcacacca aacatctgat gaatcccgaa
     2701 gtactggctg ccgcccgccg gatagtgcgc caggttgttg aagaaattat ggctcgactg
     2761 gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc gccgtcgccg ctcatttatt
     2821 ccactggcgc gaaactttga tttcaaaagt actctgcgcg ctaacctgca acactggcac
     2881 ccgcaacacg gcaagttgta tatcgaatcc ccccgcttta acagccgcat taaacgccaa
     2941 agcgaacaat ggcaactggt cttactggtt gatcaaagcg gatcgatggt ggactcggtg
     3001 atccactctg cggtgatggc ggcctgtttg tggcagttac ccggcattcg tacccatctg
     3061 gtggcgtttg acacaagcgt ggttgatctc acggcagacg ttgccgatcc ggtagagtta
     3121 ttaatgaaag tacagttggg cggcgggacc aatatcgcca gtgccgtgga gtatggtcgg
     3181 caacttattg aacaaccagc aaaaagcgtc attatcctcg tgagcgattt ttacgaaggg
     3241 ggttcatcat cattgctgac gcatcaggtg aaaaagtgtg tccagagcgg catcaaagtg
     3301 ctgggactgg ctgcgctcga tagcaccgca acaccttgct atgaccgcga tacggcccag
     3361 gcgctggtta atgtcggcgc acaaatagcc gccatgacgc cgggcgagct ggcatcatgg
     3421 cttgcggaga atcttcagtc atgaattcac tacgtccgga attattagaa ctgacaccgc
     3481 aggccctgac ggcgttaagc aatgccggtt ttgttaagcg cagtcttaag gaactggaaa
     3541 atggcaacgt cccggagatc agccatgaga acgacgcttt aatcgccacc ttcagtgacg
     3601 gtgtccgtac ccagctggcg aacggccagg cactgaaaga ggctcagtgc agttgcgggg
     3661 ccaacggtat gtgccgtcat cgcgtgatgc tggtgttaag ttatcaacga ctttgtgcca
     3721 ccactcagtc tacggaaaaa gaagaagagt gggatccggc aatctggctg gaagaactgg
     3781 ctacccttcc cgatgctacc cgcaaacgcg cacaggcgct ggtcgctaaa ggcatcacca
     3841 ttgagttgtt ctgtgcgccg ggtgaaattc cctctgcccg cttaccgatg agcgatatgc
     3901 gtttttattc ccgcagcagt attcgtttcg cccgttgtga ttgtattgaa agcacacttt
     3961 gcgaacatgt cgtactggca gtacaggcct ttgtccaggc aaaagcgcag caagcggaat
     4021 tgactcattt aatctggcag atgcgcagcg aacacgtcac atcatctgac gatccgtttg
     4081 ccagcgaaga aggcaaaacg tgtcgtcaat atgttcaaca actaagccag gcattatggc
     4141 tgagcggcat cagccagccg cttattcact acgaggccgc tttcagccgc gcgcagcaag
     4201 cagcggaacg ctgcagctgg cgatgggtga gtgaatcgct acggcaactg cgcgccagcg
     4261 ttgatgcctt ccacgcccgc gccagccact atcatgccgg agaatgctta cgtcagcttg
     4321 cggcattaaa cagtcgatta aattgcgcac aagagatggc ccggcgcgac agtattggtg
     4381 aagttcctcc tgtgccgtgg cgcacggtcg ttggctctgg cattgccgga gaagcaaagc
     4441 ttgatcatct gcggctggtg tctttaggta tgcgttgctg gcaggatatt gagcattatg
     4501 gtttacgcat ctggtttacc gatcccgaca ccggcagtat tttgcacctt tcgcgcagtt
     4561 ggccgcgaag tgaacaggaa aactcaccgg cagctacgcg tcggctgttt agttttcagg
     4621 ctggcgcact agcgggcggg caaattgttt cacaagcagc aaaacgcagt gccgatggcg
     4681 agctgctgtt agctacccgc aaccgcttaa gcagcgttgt gccgctgtcg cctgatgcct
     4741 ggcaaatgtt gagcgcgccg ttacgccagc cgggcattgt ggctttgcgg gaatatttac
     4801 gccagcgtcc ccccgcctgc atacggcctc ttaatcaggt cgataactta tttattctgc
     4861 cggtcgctga gtgtatttcg ctcggttggg atagcagccg ccagacgctg gatgcgcagg
     4921 taatcagcgg cgaaggtgaa gataatctcc tgacgttatc ccttccggca tctgcctgtt
     4981 ctccttttgc cgttgaacgc atggcggcgc ttttgcaaca aacagacgac cccgtgagtc
     5041 tggtttctgg ctttgtcagc tttgtagagg ggcaattgac actggaacca cgggtgatga
     5101 tgacaaaaac ccgcgcctgg gcgctggacg cagaaactgc gcctgtggca ccgctacctt
     5161 ctgccagcgt tttgcctgtg ccgtctaccg ctcatcagtt gctgatgcgc tgccaggcgt
     5221 tacttattca actgctccat aacggctggc gctatcagga acagagcgct attagtcagg
     5281 cagagttgct ggcgaatgac ctctccgcgg tcggttttta tcggctggca catgtgttgg
     5341 gacaatttcg taatacagaa agcgaggcac gggtagaagc aatgaataac ggtgttttac
     5401 tttgcgaaca attattcccc atgcttcagc aacaaggatg aaatagtgct ttttactaag
     5461 agttctactc cagttccgga ctgctcacgc cacggtatta ggcatatcct atatagcccc
     5521 tggtgagagt caccagttcc ttgattaaat aaaatggagt tttacatgaa ggctttcaat
     5581 aagctgtttt ccctcgttgt tgcatctgtt ctggttttct ctcttgctgg ctgcggtgac
     5641 aaagaagaat cgaagaaatt cagcgccaat ctgaacggca ctgaaattgc cattacctat
     5701 gtctacaaag gtgacaaggt gcttaagcaa tcttctgaaa ccaaaattca atttgcctcc
     5761 attggtgcaa ccaccaaaga agatgctgcc aagacacttg agccgttaag cgccaaatac
     5821 aaaaacatcg cgggtgttga agaaaaatta acctataccg atacctacgc gcaggaaaac
     5881 gtgactatcg atatggaaaa agtggatttt aaagccctgc agggtatttc aggaatcaac
     5941 gtttctgctg aagatgccaa aaaaggtatc actatggcgc aaatggaact ggtgatgaaa
     6001 gccgctggtt ttaaagaagt gaaataatct gtcggcggcc atgtttcgca tggccgccat
     6061 acccgtctta gcttttcttc acatgctggc gcgctgccag tccacgcaga aaataacgta
     6121 aaaattgatc gccgcattcg cggaagtttt tatgatccgg tgcgcgcatc atcgctgtaa
     6181 tttccggcat cgaaacgcgg aactgctgtt cggtgaggat cgccagaatg tcatcggttt
     6241 tcagcgaaaa cgcgatgcgt aattttttca gcacgatgtt gttattaatg cgacgttccg
     6301 gctccagtgc cggagcagac tcatccttgc cgcgtttttc ataaatcagg ccattgagga
     6361 atgacgacaa aacaatgtcc ggacaacgct gaaaaccctc ttcgtcttct ttacgtagcc
     6421 agacggcgat ctgttccgcg gtggcttcga cattacccag cgccagaata cgcaccaggt
     6481 cattattatt ggctttcaaa atgtagcgca cgctgcgcag aatatcgtta cttagcatga
     6541 ggccttcagg tgttgatgag gcaaaaagcc attttagcag tcttttacag gccaatcgcc
     6601 tcttttaagc ttttcagata acggcggctg accggcacgg ttaagccatt acgcaaaatc
     6661 aactcggcct ggccgttatc ttccagacga atctcctgta aatgcgcgag gttaaccaga
     6721 tactgacgat ggcagcgcag tagtggtgta cgactttcca gggtacgtaa tgtcaattcg
     6781 gtaaagccct ctttcccttc gtggctggta acgtagacac cgctcatccg actgctgaca
     6841 aatgccacat ctttcatttg cagcaaataa atccgactat gccccgtaca agggataaat
     6901 ttcagcgcct gttgattttc cggtaacagc gaaacatcct gcttgctgcg ctcctgacgc
     6961 aatcgcgcca gcgttttctc cagtcgcgct tcatcaattg gcttcagcag ataatcaaag
     7021 gcatgttctt caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa aacaatatac
     7081 gggcgatgtt ccgggtcaag catccccacc atttccagac cactgatgcg cggcatctgg
     7141 atatcgagaa acagcacatc cgggcgcagt ttatgcaccg cgccgatccc ttccacggcg
     7201 tttgaacact ctccaacgat ttcaatatcg ctctgctcct gcaaaaatac acgcaggttc
     7261 tcccgtgcta acggttcatc atcgacaatt aagactttaa tcatgcctcg tccctccatg
     7321 gtagtcgtaa cgttattcgg gtgtaactat caggctcaca ggcgacgctt attccatagt
     7381 catcgccaaa ccgttcacgt aaacgcttat ccaccagatt catccccagc ccactggcat
     7441 tggttaccgg ttgatacaaa ccggcattgt cttcgatctc cagcatcaaa tgttgcccct
     7501 cacgtcgggc gctgattgcc actcgccctg tatccagcag ttgtgatgtc ccatgtttaa
     7561 tggcgttttc cactatcggt tgcagggtaa acgcgggcaa ttgctgctgg gataattctt
     7621 gcggaatagc aatgttgacc tgcaaccgcg actggaagcg cgccttttca atttgcagat
     7681 aagcgttcac atgttcaatt tcgtcggcga gagtaacaaa ctccgaaggc cgctttaagt
     7741 ttttgcggaa aaaagtggaa agatactgca ccagctggct ggcctgttcg ctgtcgcggc
     7801 ggatcaccgc tttaatggtg ttaagcgcat taaacaaaaa atgaggattc acctgggcgt
     7861 gaagcagttt gatttctgac tgggtgagca tcgctttttg ccgctcatat tgcccggcaa
     7921 gaatctgcgc cgaaagcagt tgcgcaatcc cctcgcccag cgtgcggttt attgaactga
     7981 ataaacggtt tttggcttca tacaatttga tggtgcccat cacgcgctga ttttcgccac
     8041 gcaacggaat taccagcgtc gaccccagtt tgcattgcgg atgcaaagag caacgataag
     8101 gtacttcgtt gccatcagcg tagaccactt caccggtttc aatcgctttt aaggtgtaag
     8161 gcgaagaaat cggtttgcca ggtaaatggt ggtcgtcacc aattccggta aaggccagca
     8221 atttctctcg atcggtaatc gcgactgcac caatatccag ctcctgatac agcacctgag
     8281 ccactttcat gctgttcact tcgttaaacc cctgtcgcaa aatgccttcc gtcgaggctg
     8341 ccactttcag cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc gcgcgtttat
     8401 cgagcaatat acgcataaac agcgccgcgc cgacggtatt ggtgaccatc attggcgcag
     8461 caatattact caccagacgc accgcatctt cataaggtcg ggcgatcgcc aggatgatca
     8521 gcatttgcac catttcagcg acgaacgtga cggcaccggc ggtaatgggg ttaaagactt
     8581 tatcagtgcg cccgcgacgg atcaggatgc tgtgtaccag gccgccgagc aatccttcaa
     8641 cgatggttga gatcatgcaa cttagcgcgg tcatgccccc catcgaatat cgatgtaagc
     8701 cgccggtcag accaaccagc ccaccgacga ccggaccgcc gagtaagccg cccattaccg
     8761 cgcctatcgc acgggtattg gcaatagaat cgtcaatgtg caacccaaac caggtgccca
     8821 tgatgcagaa gatggaaaag acgatgtagc agagaaattt atgcggcaga cgaaccgtga
     8881 cctgcattaa cggtatgaat aatggcgttt tactcattaa ccacgcaatg actaaaaaaa
     8941 cgcacatctg ctgaagcagc agcaacacca gattaaaatc gtacataccc gcaaaccaca
     9001 cttcccttta aaacgcgtaa catacattgc ctgcgtttaa ctttctttga actcttgcag
     9061 aaaaatgaga attcgtgagt acgatcactc aaaatcgcct ggcaaaaata aaatcaccct
     9121 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
     9181 cgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
     9241 ctttgtgata ttaaccctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
     9301 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
     9361 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
     9421 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
     9481 cacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
     9541 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
     9601 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
     9661 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
     9721 agtctgctga cgagtgg
//
LOCUS       DAZPLI010000145         9673 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZPLI010000145 DAZPLI010000000
VERSION     DAZPLI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9673
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.146"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..1709
                     /locus_tag="ACHUU3_003407"
     CDS             <1..1709
                     /locus_tag="ACHUU3_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HFR2083391.1"
                     /translation="MRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTKL
                     SFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPWG
                     GVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGEDG
                     NGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQE
                     MNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTMT
                     LLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQS
                     TIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGDE
                     LSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDALY
                     LDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNEM
                     GELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGMQ
                     VK"
     gene            1710..3359
                     /locus_tag="ACHUU3_003408"
     CDS             1710..3359
                     /locus_tag="ACHUU3_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR2083392.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPMQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVTGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3364..4140
                     /locus_tag="ACHUU3_003409"
     CDS             3364..4140
                     /locus_tag="ACHUU3_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR2083393.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4214..5398)
                     /gene="atoB"
                     /locus_tag="ACHUU3_003410"
     CDS             complement(4214..5398)
                     /gene="atoB"
                     /locus_tag="ACHUU3_003410"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFR2083394.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5429..6751)
                     /locus_tag="ACHUU3_003411"
     CDS             complement(5429..6751)
                     /locus_tag="ACHUU3_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFR2083395.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6748..7398)
                     /gene="atoA"
                     /locus_tag="ACHUU3_003412"
     CDS             complement(6748..7398)
                     /gene="atoA"
                     /locus_tag="ACHUU3_003412"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFR2083396.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7398..8060)
                     /gene="atoD"
                     /locus_tag="ACHUU3_003413"
     CDS             complement(7398..8060)
                     /gene="atoD"
                     /locus_tag="ACHUU3_003413"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFR2083397.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8256..9641)
                     /gene="atoC"
                     /locus_tag="ACHUU3_003414"
     CDS             complement(8256..9641)
                     /gene="atoC"
                     /locus_tag="ACHUU3_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFR2083398.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
BASE COUNT         2355 a         2484 c         2604 g         2230 t
ORIGIN      
        1 agatgcgcca gacgctgacg ctgcacaaag gggcgaatta tatttcgctg gcgcagaaca
       61 ttcagcaatc tggcttgtta agcgcagaac tgcaacaaaa tgggcaagtg caggacagca
      121 ttagcacaaa actgtctttt gtggataaca gctggcccgt tgaacagcag aaaaatgtca
      181 tgctcggcgg tggcgagaac gcgctgatgt tgcccgagca ggctagcaat atccggctac
      241 aaagtagcga aacgccgcag gagattttcc gcaacaatct tgatgcgtta gtcgatggac
      301 catggggtgg cgtgatcaac accggtagcc gtctgatccc gctcagtctc gcctggcgtt
      361 cgcttgccga tcatcaaagt gccgccgcta acgacattcg tcagatgatt caggataacc
      421 gtctgcggct gatgcaactg gcggggcccg gagcgcgctt tacctggtgg ggtgaagatg
      481 gcaatggtga cgccttcctt acggcatggg catggtacgc cgactggcag gccagccagg
      541 cgctcggcgt aacgcaacaa ccggaatact ggcagcatat gctcgacagt tatgccgagc
      601 aggcggataa catgccgtta ttgcatcggg cgctggtgct ggcgtgggca caggagatga
      661 atctgccgtg caaaacgttg ttgaaagggt tggatgaagc tatcgcccgg cgcggaacta
      721 aagatgaaga tttctctgag gaagacatcc gcgatatcaa tgacagcctg atcctcgata
      781 caccggaatc tccactggca gatgcggtgg caaacgtctt aaccatgacg ttgctgaaaa
      841 aagcgcagtt gaagtccacg gtgatgccac aggttcagca atatgcgtgg gataaagcgg
      901 caaacagcaa tcagccgctg gcgcacacgg ttgtgctgct caatagcggt ggtgacgcta
      961 cccagacggc cgctatttta agtggtttga ccgctgagca atccactatt gagcgcgcgc
     1021 tggccatgaa ctggctggcg aaatatatgg cgacaatgcc tccagttgtt ttgcctgcgc
     1081 ctgcgggcgc atgggctaaa cataagttaa ctggaggggg cgaagactgg cgttgggttg
     1141 gtcagggtgt gccggacatt ctctcttttg gtgacgaatt atcgccgcaa aatgtgcagg
     1201 tccgctggcg tgaaccggca aaaacggctc aacaaagtaa cattccggtg accgttgaac
     1261 gccagttgta tcggcttatc cccggtgaag aagagatgag ctttactctg cagccggtga
     1321 ccagcaatga gattgacagc gatgcgctgt atctcgatga aattacgctt accagcgagc
     1381 aggatgcagt tctgcgctat ggtcaggtgg aagtaccgct cccgccggga gccgacgttg
     1441 agcgcacaac atggggcatt tcagtcaata aacccaacgc cgcgaaacag caggggcaat
     1501 tgctggaaaa agcgcgtaat gaaatgggcg aactggccta tatggtgccg gtgaaagaac
     1561 tgacgggaac ggtcactttc cgccatttgc tgcgcttctc gcaaaaaggg caattcgttc
     1621 tgcctcctgc tcgttatgtg cgttcctatg cacctgcgca gcaaagtgtt gcggcaggga
     1681 gtgaatggac cgggatgcag gtgaaataag tgaactggcg cagaatcgtc tggctattgg
     1741 cgttggttac tctgccaacg cttgcggagg agacgccgtt acaactggtg ctgcgcggtg
     1801 cgcagcacga tcaactgtat cagctgtcgt catcaggcgt gaccaaagtc tcagcattac
     1861 ctgactcgct caccacgccg ttagggagtt tgtggaaact gtatgtttac gcctggctgg
     1921 aggataccca tcaaccggag cagccctacc agtgtcgcgg aaactccccg gaagaggttt
     1981 attgttgtca ggctggggag agcataacgc gcgataccgc gctggtacgt tcctgtgggc
     2041 tctattttgc gccgcagcgg ttgcatattg gcgcagatgt gtgggggcaa tactggcaac
     2101 agcgtcaggc acctgcgtgg ttagcgtctc ttacaacgct gaagccagaa acctcggtaa
     2161 cggttaaatc gctgctcgat tcattagcga cgttacctgc gcaaaacaaa gctcaggaag
     2221 tcttactgga tgtggtgctg gacgaggcga aaatcggtgt cgcatccatg ctcggtagtc
     2281 gggtacgggt gaaaacctgg agctggtttg ccgatgataa acaggaaatc cgccagggtg
     2341 gttttgctgg ctggttaacc gatggtacac cgctgtgggt tactggcagt ggcaccagca
     2401 aaacggtact aacccgttat gcgacggtgc ttaatcgggt tttgccagtg ccgatgcagg
     2461 ttgccagtgg gcaatgtgta gaggtggagc tgtttgcccg ctatccgctg aagaaaatca
     2521 cagcggaaaa gagtacgacg gcagtaaaac ccggcgtact taatgggcgc tatcgtgtga
     2581 cgtttactaa tggtaaccac attacgtttg tcagccacgg tgagacgacc ttactgagcg
     2641 aaaagggcaa actgaaattg cagtcgcatc tcgatcgtga agagtacgtt gccagagttc
     2701 tggatcgcga ggcgaaaagt acgccacctg aggcagcgaa agccatgacc gtggcgatac
     2761 gtactttttt acagcaaaac gccaatcggg aaggtgattg cctgaccatc cccgacagca
     2821 gcgccacgca gcgtgtttct gcttcgccag cgaccacggg ggcgcgaacg atgaccgctt
     2881 ggacgcagga tctcatctat gcaggcgatc cggttcatta tcatggcagt cgcgctaccg
     2941 aagggacact ttcctggcgg caagccacgg cccaggcggg gcagggggag cgttacgacc
     3001 agatactcgc gtttgcttat cccgacaaca gccttagccg ctggggagct ccacgctcaa
     3061 cctgccagtt attacccaaa gcgaaagcct ggctggcgaa aaaaatgccg cagtggcggc
     3121 gtatattaca aggcgagacg gggtacaacg aaccagacgt gtttgcggtc tgtcgtttag
     3181 tcacaggttt cccctatacc gatcggcagc agaaacggct gtttattcgt aacttcttta
     3241 cgcttcagga tcggctggat ttaacccatg agtatctgca cctggccttt gatggttatc
     3301 ccaccgggct tgatgagaac tatatcgaaa cgctgacccg acaattatta atggactaat
     3361 gctatgcgaa agatttttct tccgttgtta ctggtggcac tttcgcctgt cgctcacagt
     3421 gaaggtgtgc aggaagtcga gattgacgca ccgctctctg gctggcatcc ggcagagggc
     3481 gaagatgcca gtttttcaca atcgatcaat tacccggcgt cgtccgtcaa catggcagat
     3541 gatcaaaata tctcagcgca gatccgcggc aaaataaaga attatgctgc ggcgggtaaa
     3601 gttcagcagg gccggctggt ggtcaacggt gccagtatgc cgcagagaat tgaatccgat
     3661 ggttcatttg cacgccctta tattttcact gaaggcagca acagcgtgca ggtcatcagc
     3721 ccggatgggc aaagccggca aaaaatgcag ttttactcaa cgtcgggtat aggaacgatt
     3781 cgtgcacgtt tacggctggt tctctcgtgg gatacggaca ataccgatct cgatctccat
     3841 gttgtcacgc ccgatggcga acacgcctgg tacggtaaca ccgtgctgaa aaacagtggt
     3901 gcactggata tggatgtcac gacggggtac ggacccgaga ttttcgccat gccagcacca
     3961 gttcacgggc gctatcaggt gtatatcaac tactatggtg ggcgcagcga aacggaattg
     4021 acgaccgccc aactgacgct gatcaccgat gaaggatcgg tcaatgagaa acaggaaaca
     4081 tttattgtac cgatgcgtaa tgctggcgag ctgacgctgg tgaaaagttt tgactggtga
     4141 ttaatggggt taccttcgcc gtcgatgttc aagaaaacgc ccgataactt tcgctatcgg
     4201 gtgtttttat tgattaattc aaccgttcaa tcaccatcgc aattccctgg ccgccaccaa
     4261 tgcacagtgt tgccagcccc agcgttttat cgcgtgcctg catggcatgt aatagtgtga
     4321 ccagaatacg agcaccactg gcaccgatag gatgcccgag cgcgatggcc ccgccgttga
     4381 cattcacttt ctcagaatca aagcccaggg ttttcccaac ggcgaggaac tgtgcagcaa
     4441 atgcttcatt agcttcgatg agatcaatgt ctgccagttg cagccccgcc agttgtaacg
     4501 ctttttgcgt ggcaggtact ggccccatac ccatcaatgc gggaggcacg ccaccgctgg
     4561 cataactttt aatgcgagcc aggggggtaa ggcctgctgc cagcgccgca gattcttcca
     4621 taatcaccag agcggcagca ccgtcgttaa taccagacgc gttcccagcg gtgactgttc
     4681 ctgctttatc gaaggccggg cgcaatgcac ctaacgcttc agccgttgaa ttcgctttcg
     4741 ggaattcgtc ttgactgaag acgaaggttt tcttccgagt gacaacattt accgggacga
     4801 tttcggctgt aaaagcaccg gactcaattg cggctgccgc tttacgctgt gaatgtagcg
     4861 ccagttcatc ctgcatttca cgggtaattc cgtactcttt agccacgttt tcggcggtaa
     4921 tccccatatg ataaccatgg gtggcgcaca tcaggccatc gcgcaggatt acgtcataaa
     4981 cctgtccgtc tccaagacga taaccagagc gtgcttttgc atcgagtaag tagggcgcta
     5041 aactcatatt ttccataccc cccgccacaa tgctcttcgc ctgacctgcc tgaattgcct
     5101 gggcggcaag cgccacactt ttaagacccg aaccacacac tttattgacc gtgaatccgc
     5161 acaccgtttc tgccagcccg ctttttaaca gtgcctgacg tgccggattt tgccccaacc
     5221 cggcttgcaa cacgttaccc ataatcactt catcaatgtg tagtgaatcg atttttgcac
     5281 gttcaatggc ggctttaatt actgtcgccc ccaggtcgat ggcgctggtg gaagcgagtg
     5341 aaccgttaaa actaccgata gcagtacgta ccgcactgac gatgacacaa tttttcattt
     5401 tatattcctt ctgtctgtag ggttgccgtc agaacagcgt taaaccaatg acgaaaatga
     5461 cgccggagaa gagcagggca gtgatgcaat agcccatgat gtcgcggaca ccgagtccgg
     5521 cgattgccag tgctggcagc gcccagaatg gttgttccat gttcatccat tgctcgccgt
     5581 aggcgatcgc cattaccgat ttaccgagat cagcgcccag cgcctgggct gcgggtatca
     5641 cgaaaggtcc ctgaataacc cagtgaccgc cgccagacgg aacggcgaag ttaatcagtg
     5701 cagaactaaa aaaggtcatt accgggaagg tgtctttgtt cgcaacattg atgaagaatt
     5761 cggtaatgag tccacccaga ccagaatgct ccatcatcag ttggatccca gcgtagaagg
     5821 ggaattgcac cagaataccg gcagtactgc gtgctgccgc gctgatagca cgcatataag
     5881 ccattggcgt tttatgtagc aacagacccg caatcataaa catcaggttg acggtattga
     5941 tggtgatgtt gaagccatgt tcgctgaagt acatcgccag gtaagcgata ccgagtgcgc
     6001 cgatgatcaa cgtcagaatg cggctttctt ccagtcgctc ggatggtggg gcatctttcg
     6061 gtagctgctt ttgaaaatca gcctcttcca tgagtagttc cggatcgata ctcaccacgt
     6121 cagacggttt tggcatcatc atgcgggtga taaatggcat caccacaatc aacgtcacag
     6181 tgatgaaaat gttaaaacca ctgaacagag tatcgcccac cgggatcagc ccggcgatat
     6241 gctcaaccgg gttgcccggt gttgcagcca acagaggcat tgatccagag aagccgccac
     6301 cccaggtgag aaaaccaatg taggcgcagg caatgagcaa cggataatca gaaccgggga
     6361 ctcgccgggc gacttcacgg gcaaacattg cgccgacaac caaaccaaat ccccagttga
     6421 tgacacaagc gactgaaccg aagaaagtga ccagcatgac gccctgtacg ggcgtctttg
     6481 cggcggaggc ggcagtacgc agcaaacttt tcaccggagc agagctggca agggcatgac
     6541 cggtaacgat gataagcgcc atctgcatac caaacgccag caagttccag aaaccgtcac
     6601 cccacatttt caccatgctg atcggcgttt gtggtgttaa ccaaagcgcg atcacgaatg
     6661 ttagcaatgt cagcaacatg gcaaagatca gtggatcggg aagccatcgg ctgacaaaac
     6721 gcgtcataaa acgcgatatg cgaccaatca taaatcaccc cgttgcgtat tcagatcggc
     6781 ggcgacttca aaccgagctt ctgttttggc acgcacggtg gctaaatcac acccgtcggc
     6841 aatttcggtg agccacattt tgccgtcaat aaaacgaaag acagccagtt cagtaaccag
     6901 catatgcacc gcatgttgcg cagtgagtgg catggtgcag ctgcgcaaaa tttttgctga
     6961 accatctttg gcgcaatgtt ccatggcgat gatcactttg cgcgacccgg tcaccagatc
     7021 catcgcgcca cccataccgg gcaccatttt cccaggcact acccagttcg cgaggtttgc
     7081 ttcttcgtct acttgcaaac cgccgagcac gcaggcatca atatgaccgc cacggattag
     7141 cgcaaatgac atggcgctat caaacatggc tgcaccgggt aaaacaccgc acggttgccc
     7201 gccagcgttc accagatctg gatgcgctgt cgtgaccggg cctaaaccga ggaagccgtt
     7261 ttccgattgc agagtgatat gaataccctc cggtaaataa ttggcgacca ttgtgggtaa
     7321 accgatccct aagttaacga tgtcaccatc acgaagctct tgcgccacac ggcgcgcaat
     7381 acgttgtttc gcatccatta tttgctctcc tgtgaaacga tgatgtggtc gataacggca
     7441 ccaggggtga caatatggtc aggttgcagc tcgccggttt cgaccagttc atctggctct
     7501 accagcgtga tatcagccgc aagggctatc agggggttaa agttgcgggc gctaagttga
     7561 taggtcaggt tgccaagtgt gtcgcaacga tgagcgcgaa ttagcgccag gtcggcgcgc
     7621 agtgggcgtt cgagcagcca ggttttaccg tcgagtgtca gtgtctgttt gccttcctct
     7681 acgacggtgc cgacacccgt tggggtgaga aaaccaccaa gtccagctcc accacagcga
     7741 atttgctcga ttagcgtacc ttgcggcacc agaacgacgt ccatctcacc agatatcatg
     7801 cgccgacctg tttccgggtt ggtgccgata tgtgaagcaa tcactttgcg gactcgacca
     7861 ttgacgatga gcggaccgat gccggtatca acaaacgcgg tatcattggc tatcaatgtc
     7921 aggtcgcgaa caccagattc cagtaatgct tcaaccaggc gggatggagt gccaatcccc
     7981 ataaatccgc ccaccatgat ggtcatgccg tcacgaaaga agccggtggc gtcttgtaat
     8041 gtcatcaatt ttgttttcat tttttatccc tcttgcatac accgttgtgg gtacagtcag
     8101 gcaatagcaa ggggagtgcc agaatgagat tattggattt agttatttaa ctaattgaat
     8161 atgaagggat aaattggatg gggatttata aggagaatga gcggctatgc agaaaattgc
     8221 gcactgtgca aatttctgca tagcaagttt tggtgttata catccgccgg atcgataccg
     8281 tattcctgga gtttatacat caatgcacgg cgactgatgc ccagcattaa agcagtgcgg
     8341 gttcggtttc cttcttgttg ttccagcact tccataatga tgcgtttttc gacgcgttta
     8401 atttcctctt ttaaattacg ctcaccgaca gaggctgttt ttacctcgcc agcattacag
     8461 actggctgac gaatctgtgg cggaagatcc tcagaaaaaa tgatcgggcc tgaattcatc
     8521 acgacggcgc gttcaataac gttagaaagc tctcgaatat ttcccggcca tgaccaggcg
     8581 gtaagcagtg acattgccat cggatcgatg tcgataatat cgcgctgatt ctcactactg
     8641 aatttttgca aaaagtgatt agctaacagg gaaatatctt cccggcgatc gcgcagaggc
     8701 ggcagtatta aatgaataac gttaaggcga taaaagagat cttcacggaa ggtgccttct
     8761 tttaccattg cctgcaagtc acggttggtg gcagcaatga tgcggatatc aacttttatg
     8821 gtctgatggc cgccaatccg ttcaaattcc cgttcctgta gaatgcgtag taatttggct
     8881 tgtagtacca gcggcatttc gccaatttcg tcgaggagca gagtaccttc gttggctcgt
     8941 tcaaataatc cctgacgcaa ggtttgtgca ccagtaaatg cacctttttc atgaccaaac
     9001 agttcacttt cgagcaacga ttccggcagc gccgcgcagt tgactttaat gaacggcccc
     9061 tttgcccgcc gcgaattgta gtgaatcgct ctggcaatca actctttccc ggtgccgctt
     9121 tcaccgctaa tcaagacgct ggcctgagaa agggcaattt tggcggtgtc tttgcagatg
     9181 tccatcatcg ccgggctgtt ggtgagaatg tgcccccatt gccagctggc gctcagtgcc
     9241 tggtgcagat gacggatctc ttttttcatt gactggagtt gtaaagcgcg ctgaacgatt
     9301 aaattcaact catcgagatc aaacggttta ataacatagt cgaaggctcc gcagcgtagc
     9361 gcttcgacgg cggtttccac ttccgcatag gccgtcatca gaataacggg tgtccgggtc
     9421 tcatggctgc gcatctcctt tagtgccttg atgccgtcca tctctggcat gcggatatcc
     9481 atcaacacca catcagggtg aatatcggca aacaggtgta atgctgtgcg tccgttgttc
     9541 gcacaatgtg tttcgaatcc ttgtagtgca aaagcggtgc tcagcatacg gcgaacatta
     9601 tcttcatcat ccacaataag gatgcgatta atagcagtca tacagtctga tttccctgcg
     9661 ggttgatcgg taa
//
LOCUS       DAZPLI010000146         9659 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZPLI010000146 DAZPLI010000000
VERSION     DAZPLI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9659
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.147"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(264..818)
                     /locus_tag="ACHUU3_003415"
     CDS             complement(264..818)
                     /locus_tag="ACHUU3_003415"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR2083399.1"
                     /translation="MNPKITWRLIPNSKHYYVSENGDVYNDKTKQYIATKNPSNGYKK
                     VSINGKSLYVHVLVCTLFNGAKPIGGRYTVDHIDRDKMNNKAKNLRWVTQKVNNSNRK
                     HKGTRTQKLTLKQWLMIVGEYLKGQLSQYAITKWFLNQTGINICPQNIGKLFKGTTYS
                     KWYTTYLTNKQRQRIQSLTIQRKP"
     gene            complement(873..1175)
                     /locus_tag="ACHUU3_003416"
     CDS             complement(873..1175)
                     /locus_tag="ACHUU3_003416"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083400.1"
                     /translation="MKNDFDLVQYFDDYTIVGDMDYHYFYCVKYQEGSLEAHYERIGD
                     PEVLGTITWNGNYLIDGLPMVMFVLAAEDEHKECLNRLLIVCEKARDEYRLNATGL"
     gene            complement(1165..1350)
                     /locus_tag="ACHUU3_003417"
     CDS             complement(1165..1350)
                     /locus_tag="ACHUU3_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083401.1"
                     /translation="MKKVTLKERKHHVVTARLNDDQVLVLNQLISSGKVQKDSLSACV
                     QYLINQYMILQGGGNEE"
     gene            complement(1789..2163)
                     /locus_tag="ACHUU3_003418"
     CDS             complement(1789..2163)
                     /locus_tag="ACHUU3_003418"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083402.1"
                     /translation="MRKKENKAKKEKFRQFFPENYDIGISLNEENQLRLFSKRNDSQD
                     ESLCKYEFSNKIKVQYIFLPYSSEIQVITDEFPLTEAGCQECTQAFKHPISMELIEEI
                     KEAKCSVTGLVIYSKPILKLIS"
     gene            complement(2486..4420)
                     /locus_tag="ACHUU3_003419"
     CDS             complement(2486..4420)
                     /locus_tag="ACHUU3_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR2083403.1"
                     /translation="MLRPICYERVSSIQQVDMGKGLDDQRSSLTAYLDKNADKFTDER
                     IFITDAGLSAFKNANISPESQLGIFLQDVGNRKYGVGDALVVLSLDRLSRRSSWAEST
                     IQYIVNSGIEIHDISTNLVLRVDDPMSKIHMELIQHRSHNESLMKSVRAKLAWDNKIT
                     RAVQNGEVISNRMPNWLRNVDNKYQVIPEQAELIVKCFMWYREGLSTGEIVKRIGRKW
                     QMVTVSRLIRDRRLLGEHERYNGEVVPNVYPKIIDEELFLTANRMMDRVMLEKKKPAE
                     DLLLEPEVVREIFKLYETGLGSGAIVKRLPKGWSTVNVLRVLRDKKVVTMKIIDNLTF
                     ERANEKLSRNGIANRIRKDITIAQDDYITNLFPKMLKCGHCGGNIAIHYNHVRTKYVI
                     CRNREEKKICDAKSIQYMRIEKNILQLVKNVDFNKLMMEGKSDSDSLLASLREELSTL
                     QKEAETYQVKINERKAAGKKASLPLMTGLTEVQDRIEEIESEILNSKNIKEIPVFDFD
                     INKVLDPLNVELRAKLRKEVRLVLKSVKYWVFEKRIFVQLEYFNDVLSHTLVIDNKRG
                     GGEVLHEIVISETSGERVYTVNEEGKTVFIASVTSGTDMWSLAISRTKILDDVGLYLN
                     LLKREKFEIIVNEDMIDWMD"
     gene            4500..6434
                     /gene="yeaG"
                     /locus_tag="ACHUU3_003420"
     CDS             4500..6434
                     /gene="yeaG"
                     /locus_tag="ACHUU3_003420"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR2083404.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            6547..7830
                     /locus_tag="ACHUU3_003421"
     CDS             6547..7830
                     /locus_tag="ACHUU3_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFR2083405.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKQPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            7977..9452
                     /locus_tag="ACHUU3_003422"
     CDS             7977..9452
                     /locus_tag="ACHUU3_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFR2083406.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT         2713 a         2121 c         1894 g         2931 t
ORIGIN      
        1 tcttatctct cactttaagt ttagctactg agtccttcat gatgttcttg atgtcttcgc
       61 atgggatatc tttatatagc ttgtactggc taattagtgc ttttatgttt ctgttatcaa
      121 aagttgaata ggtttttgcc atgataaata atcctgtata aattaattaa tcgtgacccg
      181 tagtgattac aggccattta ataagtttgg cttcattgcc attgagtttt gaatagatgc
      241 cagtactgga atactggcat tttttatggt ttacgttgaa tggttaggct ttgtattctt
      301 tgtctttgct tattcgtaag atatgttgtg taccattttg aataggtagt acctttgaat
      361 aacttaccta tattctgagg gcaaatatta ataccagttt gatttaaaaa ccatttagtg
      421 attgcatatt gagataattg tcctttaagg tactctccaa caatcattaa ccattgcttt
      481 agagtaagct tttgagtacg agtaccttta tgctttctat tggaattatt aaccttctga
      541 gttacccaac gtaaattttt tgctttgtta ttcattttat cgcggtcgat atgatctaca
      601 gtgtatcttc ctccaatggg tttagcacca ttgaataaag tacaaaccaa aacatgtaca
      661 tatagtgatt tgccattaat agaaactttt ttgtatccat tagatgggtt tttcgtagct
      721 atatactgtt ttgttttatc attgtaaaca tcgccatttt cagatacgta gtaatgtttt
      781 gaatttggaa ttaatctcca agtgatttta gggttcatat caaacgtcct tgttgttatc
      841 gcgtcactcc atgtgatcgc gtctgctact acttagaggc cagtagcatt tagcctatat
      901 tcatccctcg ccttctcaca tacgattagc aaacggttta aacattcctt atgttcatct
      961 tcagcagcca gtacaaacat taccattggt aatccgtcga ttaggtaatt tccattccat
     1021 gtaatagtac ctaatacttc tggatcacca attcgttcat aatgtgcttc taatgaacct
     1081 tcctgatact tcacacaata gaaatagtga taatccatat cccctactat tgtgtaatca
     1141 tcaaaatatt gtactaaatc aaagtcattc ttcattacca ccgccctgta aaatcatgta
     1201 ctgattaatt aagtactgaa cacaggctga taatgaatct ttctgtacct tcccactact
     1261 aattagctgg ttaagtacaa gtacttgatc gtcattaaga cgtgcggtta ctacatgatg
     1321 tttgcgttcc tttaaagtta cttttttcat ttctatatcc ttataggtgt atttgtatac
     1381 tgtatttata aatccaaatt aaaaagatac ctctctcgca agagaggcaa tcttaattaa
     1441 aaaggaaatt tattgtcgct atattattta tgttcaaagc tggagcggaa ggcaaaacgc
     1501 attatcgttc tgatactatt agtatagcac caaaactttt agcgtttcat ttttcttact
     1561 acgtatttat ttatcgattt gatttttaat ggggaaagca atagtactaa aatcatagat
     1621 aaaaacatga gcactatata tattgggatt atcagaaaga tgtaaagtac aaagcctact
     1681 atctcgtaaa gtaatttagt aaaccattcc attttgaagt acccccattc acattcaact
     1741 ttatagtatt tagtgaggga gtacttaaaa cgtaacaatt tggatgtgtt agctaataag
     1801 ttttagtata ggtttcgaat aaatgacaag tccagtaact gaacattttg cttcttttat
     1861 ctcttcaatt agttccattg atattggatg tttgaaagct tgagtacact cctgacagcc
     1921 tgcctccgtt agcggaaact catctgttat aacttgaatt tcagaagaat acggcaaaaa
     1981 aatgtactga actttaattt tgttggaaaa ttcgtacttg cataagctct catcttgaga
     2041 atcattccgc tttgaaaaaa gccttaattg attctcttca ttcaatgaga tcccaatatc
     2101 ataattctca gggaaaaatt gacggaattt ttcttttttt gctttatttt ctttcttacg
     2161 cattttggca atcctctctt tagaattcat ctcatggttt catcgctcct attcgttagt
     2221 gaatgacgct atggtactgg tttaacgttg aaaagtcaaa aaagttaatt ttacccttga
     2281 ctattctaac acacgctgat ataaatgatt tgctgtattt tgcacggaaa tcttaaaccc
     2341 atcgggtgcg tagttatatc caaaattcat aatccctctt ttattgctct atctacaatg
     2401 ccgttagatt tcgacatcgt agatatattg ttcaaatatt aactcgcata attcattcta
     2461 caagaccata aattctcatt agcttttaat ccatccaatc aatcatatct tcattaacaa
     2521 tgatctcaaa tttttcacgc ttgagaagat taagatacaa tccaacatca tcaagtattt
     2581 tggtacggct aatggcaagc gaccacatat cagtaccaga tgtaacggag gcaatgaaaa
     2641 cggttttgcc ctcttcattg acagtgtaga cacgctcgcc gcttgtttca gagattacga
     2701 tctcatgtag tacttcaccc ccgccccgct tgttatcaat cacaagcgta tgggatagga
     2761 catcattgaa gtattccaac tgtacaaata tacgcttttc aaaaacccag tacttaacag
     2821 atttcagtac taatcttact tctttccgta actttgcacg taactcgaca ttcaatggat
     2881 caagtacttt attaatatcg aaatcaaata cagggatttc tttgatgttc tttgaattta
     2941 atatctcaga ttctatttct tcaattctat cctgtacctc agttaaaccc gtcattaatg
     3001 gtaacgaggc tttcttacct gctgccttac gttcattaat tttaacctga taggtttctg
     3061 cctctttctg taaagtactt aactcttctc gcaatgatgc aagcaatgaa tctgaatccg
     3121 acttaccttc catcatcaat ttattgaagt ctacattttt gacaagctgt agaatgttct
     3181 tttcaattct catgtactgg atagatttag catcgcagat cttcttttct tccctgttac
     3241 gacaaataac atacttagtt cgtacatgat tatagtgaat tgcaatattg cccccacagt
     3301 gaccgcattt cagcatcttc gggaagaggt tagtaatgta atcatcctga gcaatggtaa
     3361 tgtccttacg gatacgatta gctataccat tccgcgataa cttctcatta gcccgttcaa
     3421 aggtgaggtt atcgataatc ttcatggtaa ctactttctt atcacgtagt accctcagta
     3481 cattgacggt actccaccct ttcggcaagc gttttacaat cgcccctgaa ccaaggcctg
     3541 tctcataaag tttaaaaatc tctcggacaa cttctggttc aagtaataga tcctctgctg
     3601 gcttcttctt ctccaacata acgcgatcca tcattctatt cgctgtcagg aacaactctt
     3661 cgtcgatgat tttcgggtaa acgtttggta ctacctcacc gttgtaacgt tcatgctcgc
     3721 ccaacaaacg gcgatcacgt atcagcctgg acacagtgac catctgccac tttctaccga
     3781 tacgtttaac aatctcgcca gtactcaacc cttccctata ccacatgaaa catttaacaa
     3841 tcagttctgc ctgttccggt ataacttggt acttgttatc tacatttctc aaccaattag
     3901 gcatacggtt ggagattact tccccgttct gtactgcacg tgtgattttg ttatcccatg
     3961 ccaattttgc acgtaccgat ttcatcaatg actcattgtg tgaacggtgc tgtatcagtt
     4021 ccatgtgaat ttttgacata ggatcatcaa cccgtaacac aaggtttgta gagatatcgt
     4081 gaatctcaat cccactattc acgatgtact ggatagtact ttcggcccag cttgatcgac
     4141 gcgatagacg atccagcgat aaaaccacaa gtgcgtcacc tacgccgtac ttgcggttgc
     4201 ccacatcttg caaaaatatc ccaagctgag actctggaga gatattggca tttttgaatg
     4261 cggaaagccc cgcatcagta atgaaaatcc tttcgtcagt gaacttatca gcatttttat
     4321 ctaagtatgc agtcaatgat gatcgttggt cgtccaaacc tttacccata tcaacttgct
     4381 gaatgcttga tactcgctca taacaaatag gacggagcat attttttatc ccgttgaaga
     4441 attacgctaa aagtgctgat tcacaaatgt tcccagagta tacaaaagga cggcatacca
     4501 tgaatatatt cgatcactat cgccagcgat atgaagctgc caaggacgaa gagttcacac
     4561 tgcaggagtt tcttaccact tgtcggcaag atcgcagtgc ttatgccaac gcggctgagc
     4621 ggctattgat ggctatcggt gagcctgtca tggtcgatac agcccaggaa cccagacttt
     4681 ctcgactctt ttctaaccgg gtcattgcac gttatccggc atttgaagag ttttacggca
     4741 tggaagacgc gattgaacag attgtctctt atctgaaaca cgcggctcag gggctggaag
     4801 agaagaaaca aatcctgtat ctgctggggc ctgtgggtgg gggtaaatca tcgcttgctg
     4861 agcgactgaa atcattaatg cagctcgtac cgatttatgt attgagcgcc aacggtgagc
     4921 gtagcccggt caacgatcat ccgttctgtc ttttcaatcc gcaggaagat gcgcagattc
     4981 tggaaaaaga gtatggcatt cctcgccgct atctcggcac catcatgtcg ccgtgggcgg
     5041 caaaacgcct gcatgaattt ggtggcgata tcactaagtt ccgggtagtg aaggtctggc
     5101 cgtcaattct gcaacaaatt gctatcgcca aaacagaacc cggtgatgag aacaaccagg
     5161 acatctccgc gctggtaggg aaagtcgata ttcgtaaact cgaacactac gcgcagaatg
     5221 acccggacgc ctacggctat tccggtgcgc tgtgccgcgc caatcagggg atcatggaat
     5281 ttgtcgagat gtttaaagca ccgattaaag tgctgcatcc gctgttaacc gctactcagg
     5341 aaggtaacta caacgggacg gaaggtatct ccgccctgcc gttcaacggg attattctcg
     5401 cccactcgaa cgaatccgaa tgggtcactt tccgtaataa caaaaacaac gaagccttcc
     5461 tcgaccgtgt ttacatcgtg aaggtgccgt attgcttacg catttccgaa gagatcaaaa
     5521 tctacgagaa attgcttaat cacagtgaat tgactcacgc cccatgcgcc cctggcacgc
     5581 tggaaacact gtcacgtttt tccattcttt cgcgcctgaa agagccagaa aactccagca
     5641 tttattcaaa gatgcgggtt tatgatggcg aaagtctgaa agacaccgat cccaaagcca
     5701 agtcgtatca ggaatatcgt gactacgccg gtgtcgatga agggatgaaa ggtctgtcga
     5761 cgcgttttgc gtttaagatc ctctcccgcg tgttcaactt cgatcatgta gaagtggcgg
     5821 caaacccggt ccatctgttc tacgtcctag aacagcagat cgagcgcgag cagttcccgc
     5881 aagagcaggc agaacgctat ctggagttcc tgaaaggtta tctgatcccg aaatatgccg
     5941 agtttatcgg caaagagatc cagacggcct accttgaatc ctattccgaa tatgggcaaa
     6001 acattttcga ccgttatgtt acctacgcgg atttctggat tcaggatcag gagtatcgcg
     6061 atccggatac cgggcagctg tttgaccgcg agtctcttaa cgccgagctg gagaaaatcg
     6121 agaaaccggc ggggatcagt aatccaaaag atttccgcaa cgagattgtt aacttcgtac
     6181 tgcgcgccag agcgaataac agcggacgca atccgaactg gaccagctat gaaaaactgc
     6241 gcacggttat cgagaagaaa atgttctcca ataccgagga gctgttgccg gttatctcgt
     6301 ttaacgccaa aacgtcaacc gacgagcaga agaaacacga cgactttgtc gaccgtatga
     6361 tggaaaaagg ctacacccgt aaacaggtgc gtttactgtg cgaatggtat ttgcgcgtac
     6421 gtaaatcgtc ttaacaaccc tggcccggta cacatgctac cgggcctata acgacagcga
     6481 accgtgggcc agagaaatgg caacacaggc gtagcataca gttggcaaat gtagtacggg
     6541 gggcatatga ccgggtttat tgaccggcgt ctgaacggca aaaacaaaag catggtgaat
     6601 cgccagcgtt ttttacgccg ttataaagcg caaattaaac agtcgatctc cgaggccatt
     6661 aataagcgtt cggtgactga cgtcgacagc ggcgaatccg tatccattcc cacggaagat
     6721 attagcgaac cgatgtttca tcaggggcgt ggcggcctgc gccaccgcgt acatccgggc
     6781 aatgaccatt tcgtccagaa cgaccgaatt gaacgtcccc agggtggcgg cggaggttcc
     6841 ggcagtggtc agggccaggc cagccaggat ggtgaaggtc aggatgaatt tgtctttcag
     6901 atttcgaaag atgagtatct tgatctgctc tttgaagatt tggccttacc gaatctgaaa
     6961 caaaaccaac aacgccagct gaccgaatat aaaacgcatc gggcgggtta taccgctaac
     7021 ggcgttccag ccaatatcag cgttgtgcgt tccttgcaga actcactggc gcgacgcaca
     7081 gccatgacgg cgggcaagcg acgggaactt catgcgctgg aagagaattt ggccatcatc
     7141 agcaacagtg aacctgcgca actgctggaa gaggaacgtc tgcgcaaaga aattgcagaa
     7201 ttacgtgcca aaattgaacg cgtccctttt attgacacct tcgatttacg ttacaagaac
     7261 tacgagaagc agcccgatcc ctccagccag gcagtgatgt tttgcctgat ggacgtttcc
     7321 ggttcaatgg atcaatccac taaagatatg gctaagcgtt tttatattct gctgtatctg
     7381 ttcctcagca gaacgtataa gaacgtggaa gtcgtataca tccgccatca tacccaggcg
     7441 aaagaagtcg atgaacatga gtttttctac tcgcaggaaa caggcggcac cattgtttcc
     7501 agcgccctga aactgatgga tgaggtagtg aaagagcgtt ataacccggc acagtggaat
     7561 atttacgctg cacaagcatc ggacggcgat aactgggccg atgactctcc gctttgccat
     7621 gaaatcctgg cgaaaaaatt attacctgtt gttcgttatt acagctatat cgaaattacc
     7681 cgtcgtgcac atcagacatt gtggcgagaa tatgagcatc tgcaatctac tttcgacaac
     7741 tttgcgatgc agcacatccg cgaccaggat gatatttatc cggtgttccg tgaactgttt
     7801 cataaacaaa atgcaacagc taaagactaa aactatcagc caggtcatta tcgcctggct
     7861 gatttttagc ttactgtaaa ttatctcatt tattacatac attagcttac aatcgcttta
     7921 aatatgacag cataaccttt acataattta gttccagaaa acaatcattc ggaaaaatga
     7981 ttcagtcaac acgtatttcc atggggttat tctttaaata ttttttatcg ttaacgaaaa
     8041 ttgatcctgg tcaaaactat atatctctgc catcaataaa atccagcact cacattgctc
     8101 tcctttttat ggtttctatg ggtacacaaa aattaaaagc tcaaagcttt tttattttca
     8161 gtttattgct gacgttaatt ttattttgca ttactacctt atataacgaa aacacaaatg
     8221 taaaactcat cccacagatg aattacctga tggttgttgt ggctttgttt ttccttaacg
     8281 ccgtcatttt tcttttcatg ttaatgaaat atttcactaa caaacaaatt ttaccaacac
     8341 tcattttaag ccttgcattt ttaagtggcc ttatctattt agttgaaacc attgtaatta
     8401 tccataaacc aattaacggc agtacactga tccagacaaa gtcgaatgat gtttctattt
     8461 tctatatttt ccgccaactc agttttattt gtttaacctc gctggcactc ttttgttatg
     8521 gaaaagacaa catccttgac aacaataaga aaaaaacggg aatcctgttg ctggcgctga
     8581 ttcctttttt agtttttccc cttctggcac acaatctgag cagttataac gctgactatt
     8641 ctttgtatgt cgtcgactac tgtccggaca accatactgc gacctgggga atcaactata
     8701 caaaaatatt ggtttgtctg tgggcatttt tactgttctt tattatcatg cgtacacgat
     8761 tagccagcga actatggccg ttaatagcat tattatgtct ggcatcgcta tgctgcaact
     8821 tacttctact gactctggat gagtataatt acaccatctg gtatatcagt cgcgggattg
     8881 aagtttccag taaactgttt gttgtgtctt ttctgattta taacattttt cagaaactgc
     8941 aactctccag caaactggca gttcatgatg tgctgaccaa tatttataat cggcgctact
     9001 ttttcaacag cgtagagtca ttattgtcga gacctgttgt taaggacttc tgtgtcatgc
     9061 tggttgatat taatcagttc aaacgcatca atgcccaatg gggacatcgt gtgggtgata
     9121 aagtgctggt ttcaattgtc gatattatcc agcaaagcat ccgccccgat gatattttag
     9181 cgcgactgga gggtgaggtg tttggcttgc tatttaccga actcaatagt gcccaggcaa
     9241 aaatcattgc ggaacgtatg cgtaaaaatg tcgaactcct gaccggcttt agtaacagat
     9301 atgatgttcc tgaacaaatg accatcagta ttggcacggt tttttcaacg ggtgacacgc
     9361 gtaatatctc gcttgtcatg acggaagcag ataaagcctt acgcgaagcg aaaagcgagg
     9421 ggggcaacaa agtgattatt catcatattt aagtgcaaaa atattcagag ccatgctttt
     9481 tgcgtggctt ttgcatacaa tttattacta ttacccctaa atttcccctc cccagcggcg
     9541 cagagatgag tataattagc gcccctgtgc caggccgcaa tcgaacttta tctggttttc
     9601 tcgtttcact aaccgaagga gtgccattta tcatgaaatt gcaccataga atgctccgg
//
LOCUS       DAZPLI010000147         9648 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZPLI010000147 DAZPLI010000000
VERSION     DAZPLI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9648
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.148"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..670)
                     /gene="ydcF"
                     /locus_tag="ACHUU3_003423"
     CDS             complement(<1..670)
                     /gene="ydcF"
                     /locus_tag="ACHUU3_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR2083407.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDS"
     gene            complement(942..4844)
                     /gene="hrpA"
                     /locus_tag="ACHUU3_003424"
     CDS             complement(942..4844)
                     /gene="hrpA"
                     /locus_tag="ACHUU3_003424"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR2083408.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5045..5650
                     /gene="azoR"
                     /locus_tag="ACHUU3_003425"
     CDS             5045..5650
                     /gene="azoR"
                     /locus_tag="ACHUU3_003425"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR2083409.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5701..7017)
                     /locus_tag="ACHUU3_003426"
     CDS             complement(5701..7017)
                     /locus_tag="ACHUU3_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFR2083410.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7007..8764)
                     /locus_tag="ACHUU3_003427"
     CDS             complement(7007..8764)
                     /locus_tag="ACHUU3_003427"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR2083411.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8780..>9648)
                     /locus_tag="ACHUU3_003428"
     CDS             complement(8780..>9648)
                     /locus_tag="ACHUU3_003428"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR2083412.1"
                     /translation="ALLILATLINRFLLWRLPERKGGEVTLRIRTWWGIVICFSLVIS
                     GPRWMTLAFFALISFLALKEYCMLISVHFPRWLYWVIPLNYLLIGFNCFELFLLFIPL
                     AGFLILATWRVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPSMNIQGGALLV
                     LFLLALTASNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMIASMIIGPLLT
                     PLNTLQAILAGLLIGISGFCGDVVMSAIKRDVGVKDSGKLLPGHGGILDRIDSLIFTA
                     PVFFYFIRYCCY"
BASE COUNT         2448 a         2630 c         2274 g         2296 t
ORIGIN      
        1 cgctatcatc gcgtaaacgc ggcagctcgc cagtgagtag tgagagataa cgctcaaccg
       61 gccataatcc ttgtaactga ttaataaaga ttacactgtc tgcgttattt cctaactgag
      121 gaacgaatcc gggataactt aaccagcgtg gtgcatcggg attgtcccca gtcatacggc
      181 ggaacgtcgc catcgtgcgc cgctgcatgg tggggtcctg aacaacgata gccgtatgaa
      241 ctcgttctac ggcctgattc aatagcgcga tgctaaagcg tgcgttttca ccgcagtttg
      301 ttgactggtc ttcaatccag attttttcat gcggaatgtg ccagaactga tgagcgatat
      361 ccgcctggat ggtcgcttct gctctgccag tggtgcggat agtgttgtag tgcggatgct
      421 gtgcgatggc gctatacaaa aaagttgtcg agtgaccgat accaccacta atcagtaaag
      481 gaatttgctg atcgcgagca atcttacatg ccgcgtcgat agtcggcata accgcattgc
      541 ctgcaaggat cacgcaatcg gcctgatacg gcacctcacc ggagaaatca tcctgcgcca
      601 gccactgtcc gataacattt atggcatcta tagttgccgg cgaaagcgtc ggaaacgggg
      661 taatgttcat ggttcctcct tatcgttccc ttaagaatac tgcgtgagat aatagtgaga
      721 agcggagcac tctgaaagga ggaagtcgta tttccttaac cgccactccg caagcctgac
      781 atatctttta aggaaatgct gtagataaac attattcatc ttgcagcaga gaattgtatt
      841 tttctgaatt cctgaatttt tacccttacc gttttagaat taataaaaaa cccggcgcta
      901 aatgcttacc gggttttttc tttatcaggc aaatagcagg gttaaccgct aatctgctcc
      961 atcgcctgca aaatacgctt atctgaaatc ggataaggcg taccaagttg ttgagcgaag
     1021 taactaacac gcaactcttc tatcatccaa cggatctctt tcacgtcttc atcctcacga
     1081 cgtgcaggcg gcagtttgtt gatccattgc tgccacgcct gctggacgtt ttcgactttc
     1141 agcatctgag cacggtcgcg atgtggatca accgccagtt tttccagccg tttttcaatc
     1201 gcctgcaaat atcgcagcgt gtcgcccagc cgtttgaagc cgttaccagt gacaaaaccg
     1261 cgatatacca gcccgcccat ctgcgcttta atgtcagaaa gccccagcgc catcgtcata
     1321 tccacccgcc ccttcagacg tttgttgata ttgaacactg ccgtaaggat ttgctcgacc
     1381 tgcttcgcaa tatccaccac cgtgtcgttc agttcggcac gcactttttc atgcagcgca
     1441 gcaaagcctt cttccgtcca gaccgggcca ccattggcgt cgatcaattt atccacaccg
     1501 caggagatac aatcgtcgat cagctccagc actttgccat acgggttaaa gtacagcccc
     1561 agcttggctt tgtttggtaa cttctcatgt aaatacttga tcggcgaggg aatattcagc
     1621 agcagtagac ggcgaagacc gttccacatt acctgttttt gctccagcgg attatcaaac
     1681 agtttgatcg ccacactgtc gcgttcatcc actagcgctg gccacgcctt cactttgtag
     1741 ttgccacgct tctgttcgta gctttccggc agctgaccaa aactccagat atgcagtccg
     1801 ctctgctcga taccgtcatc cgccaccgca gacagcgttt cctgcacttt gcctttcagc
     1861 gcatctttca gatcttgtag cgagcgccct tcttttagct tcttgttttt gtcatccacc
     1921 acgcggaagg tgattttcag gtgatcgggc acctgatccc agtgccagtc ttcgcggtca
     1981 acggtaacgc cggtcatccg ccgtaactcg cgctcaaggc tgtcgagcaa cggtaactcc
     2041 agcggtgtga cgcggcctaa aaacgcttcg gcgtagtttg gtgcaggcac aaaattacgg
     2101 cgtaccggtt tcggcaacga tttaatcaga gcaatcacca attcgcggcg cagaccgggg
     2161 atctgccatt caaacccgct ttcctcaacc tggttaagta acggcagcgg aatatgtacg
     2221 gtcacaccgt ccgcgtccgc gcctggctca aactgatagc tcaaacgcag cttgagattg
     2281 ccctgatgcc agaagttcgg gtaatccagc tttctgattt tttccgcccc ttctttgatc
     2341 aacatgctct tttcaaagtt gagcaaatca ggcgtttcgc ggctgacttt tttccaccag
     2401 ctatcgaagt gacgagcgga gattacatcg tggctgatgc gctggtcgta gaactcaaac
     2461 agcgtttcgt catcaaccag aatatcgcgg cgacgtgatt tgtgttccag ctcttccact
     2521 tcggcccgca gtttcaggtt ttcacggaag aatgcgtgac gcgtctgcca gtcaccttcc
     2581 accagagcgt ggcgaataaa gagttcacga cataacgccg aatcgatctg gctgtagttg
     2641 accttgcgcg cagcaacaat cggcaaacca taaacagtga ctttttccgt tgccatcacc
     2701 gcgccctgcg cccgttccca gtgcggttcg ctgtaggtgc gtttaatcaa atgctgagca
     2761 acgggttcta cccattccgg gtcgatacgc gcagcaatgc gcccccacag gcggctggtt
     2821 tctaccagtt ccgccaccat tacccatttc ggcggctttt tgaataaacc agaaccgggg
     2881 aagatggaga aacgcgcgtt acgtgcaccg gtatattcct gtttatcggc atctttcatg
     2941 ccgatatggg aaagcaaacc ggtcagtaag gcgatgtgaa tttcacgata ctccgccggt
     3001 tcgctgttga ccggaatgcc aagttctttc accacctggc gcaactgggt gtagatatcc
     3061 tgccattcgc gcacgcgcag atagttgagg taatcggtac gacacaggcg acggaaggcg
     3121 ttggaagaaa gcgccttttg ctgctcgcca agataattcc acagatttac aaacgcgagg
     3181 aagtcggatt ctttgtcgtg gaagcgacga tgtttttcgt ccgatgcctg ctgtttgtcc
     3241 atcggacgtt cgcgcggatc ctgaatggag agcgcggacg tgataatcat cgcctcacgc
     3301 acgcagccat gtttttgcgc ttccagcacc atacgcgcca gacgtgggtc gacaggcaac
     3361 tgcgagagct ggcgaccgag cggcgtcagt ttataggcgc tggcctgttc atccgtggtg
     3421 atcgcgccca gttcttcgag cagacgcacg ccatcctgga tattgcgttt atccggtgct
     3481 tcgacaaacg ggaacgcagc gatatcgccc agccccagcg cggtcatctg caaaataacc
     3541 gaggccaggt tggtacgcag aatctccgga tcggtaaact ccgggcgcga gaggaaatcg
     3601 tcttcggaat agagacgaat acagatccct tcggacacac gaccacagcg gcctttacgc
     3661 tgattggcag acgcctgcga aatgggttca atcggcaaac gctgcacttt ggtgcgatag
     3721 ctgtagcggc tgatacgcgc tgtaccgggg tcgatgacgt atttaatccc cggtacggtc
     3781 agcgaggttt ccgcgacgtt ggtcgccagc acaatgcgcc gtccgctgtg cgactggaac
     3841 acgcggttct gttcgctgtt cgaaagccgc gcataaagcg gcaagatttc ggtatggcgt
     3901 aagttcagct tgttcagcgc atcggcggta tcgcgaattt cccgctcacc actcataaag
     3961 atcagaatgt cgccagggct ttcctgactc agttcgtcta cggcgtcaaa aatcgcctgc
     4021 aactgatcgc gctcggtgtc atcggcttct tcaacaatcg gtcgatagcg cacttccacc
     4081 ggataggtcc gaccggagac ttcgataatc ggcgcattat taaagtggcg cgaaaagcgt
     4141 tccgggtcga tagtcgcgga agtgataatg atttttaggt caggacgccg cggcagcaac
     4201 tctttcaaat agccgagcag aaaatcgata ttcaggctgc gttcgtgcgc ttcgtcaata
     4261 atgatggtgt cgtactgcat cagcaggcgg tcttgctgga tctccgccag caggataccg
     4321 tcggtcatca gcttgaccat cgtgttatca cttacgtgat cgctgaaacg cactttgtaa
     4381 ccgatgcaac cgcccggctc cgttttcagc tcttccgcaa tacggttcgc caccgttctt
     4441 gccgccaggc gacgcggctg ggtatggccg atcagccctt taatcccgcg ccccagctcc
     4501 atacagattt tcggtaactg agtcgtttta ccagaacccg tttccccggc gacgatcacc
     4561 acctggtgat cacgaatcgc ttcgagaatg tcctgttttt tctgactaac cggtaaattg
     4621 tcaggataag taatttccgg tcgtgccgct tcacgcagca ggactttccc tgccgcctgg
     4681 tcaatctctt tcgccatctc ctggaaaatg gcctgttgtg catcaggatt tttaaccttc
     4741 ttcacgccgt gcagacggcg agaaaaacgc agtctgtcac gcagcatcag cgaatccagc
     4801 cgctgctgca aggccgtaaa ggtcaatttt tgttgttctg tcataacgtt agtgggcagt
     4861 agtctgcctg attaattttc tttgaaatga tagatataga gtaccacatc acggctttct
     4921 atgcgttgtt caataaattc gaacataggc ttcgatttat tgcgctatct ctgtggcagg
     4981 attgtgaata aagtgtcaac aagcaacggg gcatcgccca ttcaaacatc tataaggaaa
     5041 caccatgagc aaggtattag ttcttaaatc cagcatcctg gcagggtact ctcagtctaa
     5101 tcagttgtcc gattattttg ttgaacaatg gcgcgaaaag cactccgctg atgaaatcac
     5161 cgttcgcgac ctggctgcaa atccgattcc ggtactggat ggcgaactgg ttggtgcact
     5221 gcgtccgagc gatgcgccgc tgactccgcg tcagcaggaa gctctggcgc tttccgatga
     5281 gctgattgcc gagctgaaag cccacgacgt tatcgttatt gcggcaccga tgtataactt
     5341 caacatctca actcagttga aaaactattt tgacctggtt gcacgcgcag gcgttacttt
     5401 ccgctatacc gagaacggtc cggaaggtct ggtaacgggt aaaaaagcca tcgttattac
     5461 cagccgcggt gggatccaca aagatggacc aacggacctg gtgacgccgt atctgtccac
     5521 gttcctcggc tttatcggca ttactgatgt gaaatttgtc ttcgccgaag ggatcgcata
     5581 cggtccggaa atggcagcga aagcgcagtc tgacgcgaaa gcagccatcg acagcattgt
     5641 tgctgcataa ccggacacga tcttgatgtg gactgtagtc cagtccacat cccaatccac
     5701 ttacctgttt tcccataatt tcagcatcgc tttgtgatct tccttaagca caatatgcga
     5761 gcgtctggct cgaataaagc taatcgcttc atcaacggtt ttacagtatc cgtaacataa
     5821 cagccatgcc gccactacca gcgcactgcg cgataatccc agtgcgcaat ggaccagaac
     5881 gctgccttgc tcttcgcgta atgtttccag catcgccacg gcctgtcgga gctccccctc
     5941 ttccggaacc accagatcta gcatcggtac acagaaatag agtcgatctt ttgtcgctcg
     6001 tccacgaggg aattcaaagg tgacgtccag aaccgcattc tgtgccggaa tatgtcgtgg
     6061 aaacgcccct aaataaacac cagcagtcat tttgctcacc ggctccaggc gacgacaaaa
     6121 ccaacgcata gatagccaca tcccgatgcg ccagggcaat gtcagccagt aaacggcggg
     6181 cgggagtttc ccctgactat ctttccctgt tgttatcgcg ccaagcccac cgtacccacg
     6241 gccaataatg agtagcgata atactggcca acataaccag actgaccacc ataactgaac
     6301 catcatcatt agctccatca acacaatgca cgcgcacgcg cctacgacat atggcagtgc
     6361 tattttgatt cgacgttgat caggtttctg ataattccac cgacggtcga cgggcaccat
     6421 ccagtcaatc aacataccta ccgccagccc tgtgatgaca tcaataaaat gatgctgcca
     6481 tgtcgtcagc gtcgaaatgg cgatgagtaa aaaccatccg ccgcagactt tacgccacct
     6541 cacagccaga tgctgacgaa agtgacgcca aagtagccag cagagaataa tatgcagcga
     6601 aggagactgg ttataaggca gatcaaacag ttcaagttgc gaaaatagcc atcccgtcac
     6661 cccactcact tcaggacgga taaaactaaa cttcagcgga tagagcaaaa aaccgcagca
     6721 ggccattacc gttgccagaa taagccggtg gacaagtcgg cgctgttcga atgtcgtgct
     6781 acaaacgaac agcgaaaatc catataaaag atccagactc cagtaaggaa caatagtcca
     6841 gggaagaaaa gggatcgccg tttcccaacc gaatacctga ctgggaatat catggctatt
     6901 actgtcctga accgcggtga actgattaag agatccatag gtgaagaaga aaaacggggc
     6961 cagcaacaat aaccagccag ctccttgtag caatacgttc cggcgttcag ttatcacgac
     7021 gcaccgccat cgaaaccgta aaaatgcccc actcatcaat ccgttgtgtg catttatcaa
     7081 atccagcatc atgcacgagc aaatccatct ccccttgcgt acgcacgcgc attacccaca
     7141 gtttaccatc tttatgactg gttaacaccc cggcaatcag ctccagttgt gggtgccacg
     7201 gctgcccggt gtagatgaga atgccacccg gttcgatggc attggcaaga cctgcgagtg
     7261 agttttttac ctgctcattt tcgggaaaca gctcatacag tccagagaca atcgccagcg
     7321 taggccgcgg agttaacgcg ctgagttcct ccgggttaaa cgcatcgccc tgttcaaaac
     7381 gcacccgccc agacattccc cgttgggcaa tcatctcttg cccctgtgca acatttaact
     7441 cactgtaatc acgtaataaa atatcgctta cggcaggctc gtttgccagt gcatccagta
     7501 catagcgccc atgccctgcg gcaatgtcaa ccacgcggac ggctaaacct ttgacgtgga
     7561 gatcggcaac ggcttgttta atcagtattt gtaaatgagt tttgcgctgg cgaataccgc
     7621 gccagccaac actgttcagg tagattttgt cgattaagcg cccgaatgcg ttactgcctt
     7681 gcggttgatt ttgatagaca taatccagcg aactgcctga atcaaagcca gtgctcattc
     7741 cgagatgcag gcccgcagag tgcgtcccga acaatttcat cgctttacgc ataaagcgat
     7801 acgccaaatc aaccggcgat aatggcacgg gtccacctga aaggaggcgc cagcgatccg
     7861 ctgatggtcc agtgcggtct tcatgctgat aatcaaattt ttgtgacttg ttagcatata
     7921 aacgacgaat aaagctttgc attttttcaa atgcctgcgc cctgttctct tcacccaacg
     7981 tgtcgtgata aaagcctggc agcagatgca gctctttcag agggctacgt aatctctgat
     8041 aaaaatcaat ctgcggttgg cgatgcacca catagtcatc gcctgatatc agaagttgcg
     8101 tggggagcgt aatcgccgcc gcatctctaa taatacgttc agacgtttta tagagatcga
     8161 gcaagatatt gacggcaatc gcccgtgtga tcagcggatc attattgaaa ctcgccaccc
     8221 gttgccgatc gtgggtcaaa tagcgtcttt tcacatagga attaataaaa aacagaccac
     8281 gcagacgatg ccatagcgtc agcgcaggac gtgccagcgg cacatacaat ttaaccttaa
     8341 aggccggaga agccagcacc agcccgcgaa ttgcaggcgc ataatcatga acccatgtgg
     8401 caaccagcac tgcgccgacg ctttgcgcga tcacaaccac ctcttccagt ccaacctggc
     8461 tgtcgctggc agcaaaacgg acaaattcat cgacatcctg cactgaacgc gcaagagatg
     8521 ggctataacc acgcggcccc gaagtttttc catgcccccg ggcatcccat gcataaaaag
     8581 cagtatctgg catcgccagt tcatcaacga tatgttgtag acgaccagaa tgttcatggc
     8641 cacgatgaaa taagacgatg acctttttcg cccctggctg taaagcgggc cagtgccgat
     8701 aaaacaacgc tgtattatca ctggtggtaa aaaaatgttc cccagagatg cgtgaatttt
     8761 ccattttctc tattttcctt cagtaacagc agtaacgaat aaaataaaaa aataccggag
     8821 cggtgaaaat taatgagtca atcctgtcaa ggattccgcc atgtccaggt aatagtttcc
     8881 cgctatcttt aacaccaaca tctcgtttga tggctgacat cactacatcg ccgcaaaaac
     8941 cactaatacc aattaacaaa cccgctaata ttgcctgtaa tgtatttagc ggtgtcagta
     9001 acggtccaat gatcatcgac gctatcatgg tggtgattac gccgccgacc agaccttcca
     9061 gcgtttttcc cgggctgact ttggggacca cttttcttct gccgcaggat ttcccccata
     9121 aatactgtgc aatatcgttt gacgcggtta atgccaaaag aaataacacc agtaaggccc
     9181 cgccctggat attcatggat ggcaacatta ataaccaggc ggcatgactc aaggcgaaaa
     9241 cggtcattat ccagccccaa aaaatggcgc tcacggtatg cagaaaaccg gaggggtcac
     9301 ccacaaaaac tcgccaggtg gctaatatca gaaacccagc caaaggtata aataacagaa
     9361 acagctcaaa gcaattaaaa ccgataagca aatagttaag aggaataacc caatataacc
     9421 accgcggaaa atgtacggat ataagcatac aatattcttt caatgccaga aaacttatca
     9481 gcgcaaaaaa cgccaacgtc atccagcgtg gacctgaaat caccagtgaa aaacaaatga
     9541 cgatgcccca ccaggtacga atacgtaatg taacctcacc cccttttctc tccggtaacc
     9601 gccatagcag aaaacggttt atcagcgttg ctaaaattaa caacgcga
//
LOCUS       DAZPLI010000148         9619 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZPLI010000148 DAZPLI010000000
VERSION     DAZPLI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9619
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.149"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(29..544)
                     /gene="ompX"
                     /locus_tag="ACHUU3_003429"
     CDS             complement(29..544)
                     /gene="ompX"
                     /locus_tag="ACHUU3_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR2083413.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(597..662)
                     /locus_tag="ACHUU3_003430"
     CDS             complement(597..662)
                     /locus_tag="ACHUU3_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083414.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            897..1784
                     /gene="rhtA"
                     /locus_tag="ACHUU3_003431"
     CDS             897..1784
                     /gene="rhtA"
                     /locus_tag="ACHUU3_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR2083415.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            2083..2586
                     /gene="dps"
                     /locus_tag="ACHUU3_003432"
                     /gene_synonym="pexB"
     CDS             2083..2586
                     /gene="dps"
                     /locus_tag="ACHUU3_003432"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR2083416.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            2990..3736
                     /gene="glnH"
                     /locus_tag="ACHUU3_003433"
     CDS             2990..3736
                     /gene="glnH"
                     /locus_tag="ACHUU3_003433"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR2083417.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            3875..4534
                     /gene="glnP"
                     /locus_tag="ACHUU3_003434"
     CDS             3875..4534
                     /gene="glnP"
                     /locus_tag="ACHUU3_003434"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR2083418.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            4531..5253
                     /gene="glnQ"
                     /locus_tag="ACHUU3_003435"
     CDS             4531..5253
                     /gene="glnQ"
                     /locus_tag="ACHUU3_003435"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR2083419.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            5370..7595
                     /gene="ybiO"
                     /locus_tag="ACHUU3_003436"
     CDS             5370..7595
                     /gene="ybiO"
                     /locus_tag="ACHUU3_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFR2083420.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(7592..8518)
                     /gene="rlmF"
                     /locus_tag="ACHUU3_003437"
     CDS             complement(7592..8518)
                     /gene="rlmF"
                     /locus_tag="ACHUU3_003437"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR2083421.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            8794..9054
                     /gene="mcbA"
                     /locus_tag="ACHUU3_003438"
     CDS             8794..9054
                     /gene="mcbA"
                     /locus_tag="ACHUU3_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR2083422.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            9319..>9619
                     /locus_tag="ACHUU3_003439"
     CDS             9319..>9619
                     /locus_tag="ACHUU3_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR2083423.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         2303 a         2425 c         2407 g         2484 t
ORIGIN      
        1 aggcggattt tttatatcac caaagtgatt agaagcggta accaacaccg gcaatccagg
       61 tgcctacgtc aacgctacga atacggctct gctcgtaaga gaagtccaga gcaacgtttt
      121 ccatcgggtt gaactgcaga ccagcaccgt aggagaaacc gtagtcgctg gtgtcgtgtt
      181 tgtaggtcgg gtattcagtg gtctggaatt taccataacc cacccccact acaccgtaga
      241 tgcttgccca gtcgttaatg cggtaagccg gaccagcagt gatgccgtag tactggtttt
      301 tgttgtagtc accagagctt gcagtacggc ttttctcggt gtaagtgaaa gaaccgatca
      361 cacccagcgg gctgttgtct tcttcatagc ggtatttcag gttgaaaccg cccattttgt
      421 tcatttggcc ctgagcgtcg ctctgtgcgt aaccgccagt tacggtagaa gtcgcagcta
      481 cggaagtacc tgcggtgaaa gccagaactg cggccagtgc tgaaagacat gcaatttttt
      541 tcataaccac ctcaaatgtg attcaaataa gtcctaagtt ttaaatatat caaaaattaa
      601 tgggaaactc ttcgcgattt gtgatgtcta acgggccatt tcatgtaaca gaacgtttcc
      661 atacaccgct atccatctaa atttaaatca ctttttcaga gaactgcgta agtattacgc
      721 atgttttccc tgtcattcat ccagattatt cctaatcacc agactaatga ttccatcaat
      781 cctggcgcat tttagtcaaa acgggggaaa attttttcaa caaatgctca accagcattg
      841 ggtatatcca gtacactcca cgctttactt aagtctagat atttgtggga gaaaggatgc
      901 ctggttcatt acgtaaaatg ccggtctggt taccaatagt catattgctc gttgccatgg
      961 cgtctattca gggtggagcc tcgttagcta agtcactttt tcctctggtg ggcgcaccgg
     1021 gtgtcactgc gctgcgtctg gcattaggaa cgctgatcct catcgcgttc tttaagccat
     1081 ggcgactgcg ctttgccaaa gagcaacggt taccgctgtt gttttacggc gtttcgctgg
     1141 gtgggatgaa ttatcttttt tatctttcta ttcagacagt accgctgggt attgcggtgg
     1201 cgctggagtt caccggacca ctggcggtgg cgctgttctc ttctcgtcgc ccggtagatt
     1261 tcgtctgggt tgtgctggcg gttcttggtc tgtggttcct gctaccgctg gggcaagacg
     1321 tttcccatgt cgatttaacc ggctgtgcgc tggcactggg ggccggggct tgttgggcta
     1381 tttacatttt aagtgggcaa cgcgcaggag cggaacatgg ccctgcgacg gtggcaattg
     1441 gttcgttgat tgcagcgtta attttcgtgc caattggagc gcttcaggct ggtgaagcac
     1501 tctggcactg gtcggttatt ccattgggtc tggctgtcgc tattctctcg accgctctgc
     1561 cttattcgct ggaaatgatt gccctcaccc gtttgccaac acggacattt ggtacgctga
     1621 tgagcatgga accggcgctg gctgccgttt ccgggatgat tttcctcgga gaaacactga
     1681 cacccataca gctgctggcg ctcggcgcta tcatcgccgc ttcaatgggg tctacgctga
     1741 cagtacgcaa agagagcaaa ataaaagaat tagacattaa ttaaatttac atttctgcat
     1801 ggttatgcat aaccatgcag aatttctcgc tacttttcct ctacaccgtc tttatatatc
     1861 gaattatgca aaagcatatt tattccgaaa attcctggcg agcagataaa taagaattgt
     1921 tcttatcaat atatctaact cattgaatct ttattagttt tgtttttcac gcttgttacc
     1981 actattagtg tgataggaac agccagaata gcggaacaca tagccggtgc tatacttagt
     2041 ctcgttaatt actgggacat aacatcaaga ggatatgaaa ttatgagtac cgctaaatta
     2101 gttaaatcaa aagcgaccaa tctgctttat acccgcaacg atgtctccga cagcgagaaa
     2161 aaagcaacag tagagttgct gaatcgccag gttatccagt ttattgatct ttctttgatt
     2221 accaaacaag cgcactggaa catgcgcggc gctaacttca ttgccgtaca tgaaatgctg
     2281 gatggcttcc gcaccgcact gatcgatcat ctggatacca tggcagaacg tgcagtgcag
     2341 ctgggcggtg tagctctggg gaccactcaa gttatcaaca gcaaaacccc gctgaaaagt
     2401 tacccgctgg acatccacaa cgttcaggat cacctgaaag aactggctga ccgttacgca
     2461 atcgtcgcta atgacgtacg caaagcgatt ggcgaagcga aagatgacga caccgcagat
     2521 atcctgaccg ccgcgtctcg cgacctggat aaattcctgt ggtttatcga gtctaacatc
     2581 gaataaatcc atcgctgatg gtgcagaact ttagtacccg ataaaagcgg cttcctgaca
     2641 ggaggccgtt ttgttttgca gcccacctca acgcacttat ttagtgcatc catctgctat
     2701 ctccagctga ttaagtaaat tttttgtatc cacatcatca cacaatcgtt acataaagat
     2761 tgttttttca tcaggtttta cgctaaataa tcactgtgtt gagtgcacaa ttttagcgca
     2821 ccagattggt gccccagaat ggtgcatctt cagggtattg ccctataaat cgtgcatcac
     2881 gtttttgccg catctcgaaa aatcaaggag ttgcaaaact ggcacgattt tttcatatat
     2941 gtgaatgtca cgcaggggat cgtcccgtgg atagaaaaaa ggaaatgcta tgaagtctgt
     3001 attaaaagtt tcactggctg cactgaccct ggcttttgcg gtttcttctc atgccgcgga
     3061 taaaaaatta gttgtcgcga cggataccgc cttcgttccg tttgaattta aacagggcga
     3121 taaatatgtg ggctttgacg ttgatctgtg ggctgccatc gctaaagagc tgaagctgga
     3181 ttacgaactg aagccgatgg atttcagtgg gatcattccg gcactgcaaa ccaaaaacgt
     3241 cgatctggcg ctggcgggca ttaccatcac cgacgagcgt aaaaaagcga tcgatttctc
     3301 tgacggctac tacaaaagcg gcctgttagt gatggtgaaa gctaacaata acgatgtgaa
     3361 aagcgtgaaa gatctcgacg ggaaagtggt tgctgttaag agcggtactg gctccgttga
     3421 ttacgcgaaa gcaaacatca aaactaaaga tctgcgtcag ttcccgaaca tcgataacgc
     3481 ctatatggaa ctgggcacca accgcgcaga cgccgttctg cacgatacgc caaacattct
     3541 gtacttcatc aaaaccgccg gtaacggtca gttcaaagcg gtaggtgact ctctggaagc
     3601 gcagcaatac ggtattgcgt tcccgaaagg tagcgacgag ctgcgtgaca aagtcaacgg
     3661 cgcgttgaaa accctgcgcg agaacggaac ttacaacgaa atctacaaaa aatggttcgg
     3721 tactgaaccg aaataataac gctacacctg taaagcgcac tggcagttcc ctctcccctc
     3781 tggggagagg attagggtga ggggcgcaaa cccgctccgg ggccattaat taccctgaat
     3841 ttgattattt acaccacggt aacaggaaca acatatgcag tttgactgga gtgccatctg
     3901 gcctgccatt ccgcttctga ttgaaggtgc caaaatgacc ctgtggattt cggtcctcgg
     3961 tctggctggc ggtctggtaa tcggattgct ggcaggtttt gcacgcacct tcggtggttg
     4021 gatagccaac cacgtcgcgc tggtctttat tgaggtgatc cgcggcacac ctatcgtcgt
     4081 ccaggtgatg tttatttatt tcgccctgcc gatggcgttt aacgacttac gcatcgaccc
     4141 atttactgcg gcggtggtca ccatcatgat caactccggc gcgtacattg cggaaatcac
     4201 gcgtggtgcg gtgctgtcta ttcacaaagg ttttcgtgaa gcaggactgg cgctcggtct
     4261 ttcacgttgg gaaaccattc gctacgtcat tttaccgctg gcactgcgtc gtatgctgcc
     4321 gccgctgggt aaccagtgga tcatcagcat taaagacacc tcgctgttta ttgtgatcgg
     4381 cgtggcggaa ctgacccgtc aggggcaaga aattattgcc ggtaacttcc gcgcccttga
     4441 gatctggagc gccgtggcgg tgttctatct gattattacc ctggtgctaa gctttatttt
     4501 gcgtcgtctg gaaagaagga tgaaaatcct gtgattgaat ttaaaaacgt ctccaagcac
     4561 tttggcccaa cccaggtgct tcacaatatc gatttgaaca ttgcccaggg cgaagtcgtg
     4621 gtgattatcg ggccgtccgg ttccggtaaa tcgaccctgc tgcgctgcat caacaaactg
     4681 gaagaaatca cctccggcga tctgattgtc gatggcctga aggttaacga tccgaaagtt
     4741 gacgagcgcc tgattcgcca ggaagcaggt atggtgttcc agcagtttta cctcttcccg
     4801 catctgacgg cgctggaaaa cgtcatgttt ggtccgctac gcgtgcgtgg cgcgaacaaa
     4861 gaagaggcgg aaaaactggc gcgtgagctg ctggcgaaag tcggtctggc agaacgtgca
     4921 catcactacc cttccgaact ttctggtggt caacagcagc gtgtggcgat tgcccgcgcg
     4981 ctggcggtga agccgaaaat gatgctgttt gatgaaccga cttccgctct tgacccggaa
     5041 ctgcgccatg aagtgctgaa ggttatgcag gatctggctg aagaagggat gacgatggtg
     5101 atcgtgaccc acgaaatcgg ttttgccgag aaagtagctt cgcggctgat ctttatcgac
     5161 aaaggccgga ttgcggaaga tggcgatccg caggtattga tcaagaaccc gccgagccag
     5221 cgcttgcagg aatttttgca gcacgtctct taacttctct gcctcccttt cacccgaaag
     5281 ggaggcacac cagattcctc tcatttaaaa tcgcccctcc cccagcatct atacttatct
     5341 ttttgctctg ttttctcact ggaggagtca tgcggtggat cctgttcatc ctcttctgcc
     5401 tgctgggcgc acctgcccac gcggtatcca tacccggcgt tacaaccaca acgacaacgg
     5461 actcaacgac tgaaccggcc ccggaaccgg atatcgaaca aaaaaaagcg gcctatggcg
     5521 cactggcgga tgtgctggat aacgacacct cgcgtaaaga attgatcgac cagttgcgta
     5581 ctgtcgccgc gacgccccct gcggaaccgg tcccgaagat cgtgccgccg acgctggttg
     5641 aagagcaaac cgtgctgcaa aaggtcaccg aagtcagccg ccattatggt gaagcccttt
     5701 ccgcccgctt cgggcaactt tatcgcaata tcaccggctc cccgcataag ccatttaatc
     5761 cacaaacctt cagcaatgcg ctgagccatt tttcaatgtt agcggtatta gtgtttggtt
     5821 tttactggct gattcgcctg tgcgcactgc cgctgtatcg caaaatgggt cagtgggcga
     5881 ggcaaaaaaa tcgtgagcgc agcaactggt tgcagcttcc ggcgatgatt atcggggcgt
     5941 ttattatcga cctgctgtta ctggccctga cattatttgt cggccaggta ttaagcgaca
     6001 acctgaatgc aggcagtcgc accatcgctt tccaacaaag tttgtttctc aacgcctttg
     6061 ccctgattga atttttcaaa gccgtactac gcctgatttt ttgcccaaac gtggcggagc
     6121 tgcgcccgtt cacgattcag gacgagagcg cccgttactg gagtcgtcgc ctgagctggt
     6181 taagcagttt gataggttat ggcctgattg tggccgtgcc gattatctct aatcaggtga
     6241 atgtacagat aggtgcgctg gcgaacgtca tcattatgct gtgcatgacc gtctgggcgt
     6301 tgtacctgat ctttcgtaat aaaaaagaga ttacccagca tttgctcaac tttgcggagc
     6361 attcgctggc ctttttcagc ctgtttatcc gcgcctttgc gctggtgtgg cactggctgg
     6421 caagcgccta ttttatcgtg ctgtttttct tttcgttgtt cgatccgggc aacagcctga
     6481 aatttatgat gggtgcaacg gtgcgcagcc tggcgattat tggtatcgca gcgtttgttt
     6541 ccggtatgtt ttcccgctgg ctggcaaaaa ccatcactct ctcgccacat actcagcgta
     6601 actatccaga gttgcaaaaa cggctgaatg gctggctttc ggcggcgctg aaaacggcgc
     6661 gtattctgac cgtctgcgtg gcggtaatgt tgctgttgag cgcatgggga ttgttcgatt
     6721 tctggaactg gctgcaaaac ggcgcggggc agaaaaccgt agatatcctg atccgtatcg
     6781 cacttattct ttttttctcg gcggttggct ggacggtgct cgccagtttg atcgaaaacc
     6841 ggctggcttc ggatattcat ggccgcccgc tacccagcgc ccgtacgcgt accctgctga
     6901 cgctgttccg taacgcgctg gcggtgatta tcagtaccat caccatcatg attgtgttgt
     6961 cggaaatcgg cgtcaatatc gcgccattgc tggcaggtgc cggggcatta ggtctggcta
     7021 tctcgtttgg ttcgcaaacg ctggtgaaag atatcattac cggggtgttt attcagtttg
     7081 aaaacggcat gaacactgga gatttggtga ctatcgggcc gttgaccggc acagtggaac
     7141 ggatgtcgat tcgctccgtg ggcgtgcgcc aggataccgg ggcgtatcac atcattccgt
     7201 ggtcttcgat aaccaccttt gctaacttcg tccgcggtat tggttcggta gtggcaaact
     7261 acgatgttga tcgccatgaa gatgctgata aagccaatca ggcactgaaa gatgcggtag
     7321 cggaattaat ggaaaacgaa gaaattcgcg ggctgattat tggtgaaccg aattttgccg
     7381 ggattgtcgg cttaagcaat accgcgttta cactgcgtgt ttcgttcacc acgctgccac
     7441 tcaaacagtg gacggtacgc tttgccctcg acagccaggt gaaaaaacat ttcgacctgg
     7501 cgggcgttcg cgcgccagtg cagacttatc aggtgctgcc tactccgggc gcgaccccgg
     7561 ctgaaccgtt accgccgggg gagccaacgc tttaacgctg gcgattgaca aaacggcggc
     7621 gctgctcgtc gttcataaag gtccaggcaa taaagcgact ctgcttttgc ccctgggcca
     7681 tctctttttt taccaccttc accgcgccca cgtcggtcag agcacgatac aacggcggta
     7741 agttttcacc acgagatacc agcgaggtaa accacatcac ctgcttcgcg aagcctttgc
     7801 tctcttcaat catctttttg ataaaggcga cttcaccacc ttcacaccac aactcctgtt
     7861 gctgaccacc aaagttcagt gcatcgtctt tgttcagccc caggttacgg cgtttacgct
     7921 cactacctgc ccgcgctgca gcggcggaat cgtggaatgg cgggttacac aaggtcgcgt
     7981 cgtattgctc gtttttatgg atgatgccgt taaaaattgc cccactctct ttttgccgac
     8041 gcagacgaat ggcgcggtta agccccggat tagcactgat aatcgcctgc gcactgctta
     8101 acgcctggct gctggtttca ctaccggtaa aacgccagcc atattcatgt acgccaatca
     8161 gcggataaat acagttcgca ccaacgccga tatccagaat gctggcattc gctggaattg
     8221 ttccgcttgc ttcagccagt aaatcggcaa ggtgatgaat ataatccgcc cggcccggta
     8281 ctggtgggca gagaaaaccg tcggggatat cccagttcgc tacggcgtaa aaatgggcca
     8341 gcaacgcctt attgagcgcc ttcaccgcca gcggattggc aaagtctacg ctttgctccc
     8401 cggcgggtgt aagcgtgagg aattgcctga gttcaggatt gacctgacaa agcgtggcga
     8461 gatcgtagcg gctgtgatga cggttgcgcg gatgcaaccc cggtttctgg gcggacatag
     8521 cattctcctt taagcagcgg cgtaagatac ccgcataacg cgacagggta aatatctggc
     8581 tgcgtttatc attccccaaa accttaacat ggctatggtt tatggcaatt catcataaaa
     8641 atcagcatta tgcatgtcat taatttgtct tattttgtaa aaatatatcg ttcaaaaaat
     8701 aagcacatct ctgtcaattt tttgcgtcat ttgcatcgcg caggcgacct acacttatca
     8761 ggcactaccc gttgtattta tgaggatgtc attatgaaaa agtgcctcac actactgatt
     8821 gccaccgtcc tgagcggaat ctctctcacg gcttatgccg cgcaaccgat gagtaacctg
     8881 gacagcggtc agctacggcc cgccggtacc gtttcggcaa ctggcgcatc aaacctaagc
     8941 gatctggagg ataaactggc agaaaaagcg cgcgaacaag gcgcgaaagg ttatgtcatt
     9001 aattccgcag gcggaaatga ccagatgttc ggtactgcaa ccatctacaa ataaccccac
     9061 caacgtaact gcattcgcct ctggatgcag ttttcgcctg ttttgtaatc aaaaaacatc
     9121 aaatatgaac tcaatgtaaa taaatgtatt tctttttcgc gcaatgggtg atagaaaatc
     9181 gcgccaagtg ataatgctta tcaaaattat tatcacttgc acgaacacta tcacgggatt
     9241 aacagtggca tcgcatccgc agagatgctt tctcgtggca gtgaaaattt caacatataa
     9301 gaaaaagtca cctgcaaaat ggaaaacaat cgcaatttcc ctgccagaca atttcattcg
     9361 ctcacgttct ttgccggtct ttgtattggc atcacgcctg tggctcaggc actcgccgcc
     9421 gaagggcaaa ctaacgcgga tgacacgctg gttgtcgaag catcaacgcc ttcgctttat
     9481 gcgccacaac aatctgccga tccgaaattc tcgcgtccgg tagcggatac tacccgcacg
     9541 atgacggtga tttctgaaca agtgattaaa gatcagggcg caaccaacct taccgacgcg
     9601 ctcaaaaacg cccccggcg
//
LOCUS       DAZPLI010000149         9567 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZPLI010000149 DAZPLI010000000
VERSION     DAZPLI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9567
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.150"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            417..1394
                     /gene="glaH"
                     /locus_tag="ACHUU3_003440"
     CDS             417..1394
                     /gene="glaH"
                     /locus_tag="ACHUU3_003440"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR2083424.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1414..2682
                     /gene="lhgO"
                     /locus_tag="ACHUU3_003441"
     CDS             1414..2682
                     /gene="lhgO"
                     /locus_tag="ACHUU3_003441"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR2083425.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2705..4153
                     /gene="gabD"
                     /locus_tag="ACHUU3_003442"
     CDS             2705..4153
                     /gene="gabD"
                     /locus_tag="ACHUU3_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR2083426.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4167..5447
                     /gene="gabT"
                     /locus_tag="ACHUU3_003443"
     CDS             4167..5447
                     /gene="gabT"
                     /locus_tag="ACHUU3_003443"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFR2083427.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5758..7158
                     /gene="gabP"
                     /locus_tag="ACHUU3_003444"
     CDS             5758..7158
                     /gene="gabP"
                     /locus_tag="ACHUU3_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR2083428.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7179..7841
                     /gene="csiR"
                     /locus_tag="ACHUU3_003445"
     CDS             7179..7841
                     /gene="csiR"
                     /locus_tag="ACHUU3_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR2083429.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7842..8291)
                     /gene="kbp"
                     /locus_tag="ACHUU3_003446"
     CDS             complement(7842..8291)
                     /gene="kbp"
                     /locus_tag="ACHUU3_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR2083430.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8375..8533)
                     /locus_tag="ACHUU3_003447"
     CDS             complement(8375..8533)
                     /locus_tag="ACHUU3_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR2083431.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8716..9015
                     /locus_tag="ACHUU3_003448"
     CDS             8716..9015
                     /locus_tag="ACHUU3_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR2083432.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            9025..9549
                     /gene="ygaP"
                     /locus_tag="ACHUU3_003449"
     CDS             9025..9549
                     /gene="ygaP"
                     /locus_tag="ACHUU3_003449"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR2083433.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
BASE COUNT         2203 a         2457 c         2624 g         2283 t
ORIGIN      
        1 actctaaata tcaatattcc tgggcgtagc gctcttcttt tagggaaaac aggagaaccg
       61 ccgaactatc tctatttata atttaatgtt atatctgccc cgataaaacg gggcagataa
      121 tatgtttagt ttactaacgg tcattttgca gtgaagccat ttactgtttt ttatcgacca
      181 gataatctgt tctctaatgt taactccccc taacctgttg ctttagttat tcatttcctg
      241 tctcactttg ccttaatact ctacgttaaa tgttactaat ttgttgcttt tgatcacaat
      301 aagaaaacaa tatgtcgctt ttgtgcgcat ttttcagaaa tgtagatatt tttagattat
      361 ggctacgaaa tgagcatcgc catgtcaccc tacatctcat aagaggatcg cttctgatga
      421 atgcactgac cgccgtacaa aataacgctg tcgattcagg ccaggactat agcggattca
      481 ctctcatccc gtcggcgcaa tccccgcgtc tgctggaact caccttcacc gaacagacga
      541 ccaaacagtt tctcgagcag gttgccgaat ggcccgtaca ggcgctggag tacaaatcgt
      601 ttctgcgttt tcgggtaggc aaaattcttg acgatctgtg tgcgaatcag ctgcaaccat
      661 tgctgttgaa gaccctgcta aaccgtgctg aaggtgcgct gttgatcaat gcggtgggta
      721 tcgatgatgt ggcgcaggca gatgagatgg tgaagctggc aacggcggta gcgcatctga
      781 ttggtcgctc aaatttcgat gcgatgagcg gtcagtatta cgcgcgtttc gtggtgaaaa
      841 atgtcgataa ctcagacagc tatctgcgtc agccgcaccg cgtgatggaa ctgcacaacg
      901 acggcacgta tgtcgaagag atcaccgatt acgtgctgat gatgaaaatc gatgagcaaa
      961 acatgcaggg tggaaattcg ttgctgctgc atctcgatga ctgggaacat ctggaccact
     1021 atttccgcca tccactggcg cgtcgcccga tgcgctttgc cgcgccgccg agcaaaaacg
     1081 tcagcaaaga tgttttccat ccagtgttcg acgttgatca acagggtcgc ccggtgatgc
     1141 gctatatcga ccagttcgtc cagccaaaag attttgaaga aggcgtgtgg ttgagcgagc
     1201 tttccgatgc cattgaaacc agcaaaggca ttctttctgt acccgttccc gttggcaaat
     1261 tcctgttgat aaacaacctg ttctggctgc acggtcgcga ccgctttact ccgcacccgg
     1321 atctgcgccg tgaactgatg cgtcagcgtg gctatttcgc ttacgccact caccactacc
     1381 agacgcatca gtaagcgcaa aggaattgag cggatgtatg attttgtgat tattggcggc
     1441 ggcatcatcg gcatgtcgac cgccatgcaa ctgattgatg tttatccgga tgcccgcatt
     1501 gcgttgctgg aaaaagagtc cggcccggcc tgtcaccaga cgggccacaa cagcggcgtg
     1561 atccatgccg gggtctatta cacgcccggc agcctgaagg cgcagttttg cctggcgggg
     1621 aaccgcgcca ccaaagcctt ttgcgatcaa aacggcattc gctatgacaa ctgcggcaag
     1681 atgctggtcg ccacgtctca gctcgaaatg gaacggatgc gcgccttatg ggaacgcaca
     1741 gcggcgaacg gtatcgagcg cgagtggata aacgccgtgg aactgcgcga gcgcgaaccg
     1801 aatatcaccg ggcttggtgg catttttgtg ccgtccagcg gcattgtcag ctaccgcgaa
     1861 gtcacagcgg caatggcaaa aatcttccag gccagaggcg gcgagattat ttataacgcc
     1921 gaagtcagcg ccctcagtga gcataaaaac ggcgtggtga tacgcacccg tcagggcggt
     1981 gaatatgaag catcgacgct gattagctgt tccgggctga tggctgaccg gctggtgaaa
     2041 atgctcggcc tcgaaccggg ctttatcatc tgcccgttcc gtggtgagta tttccgcctt
     2101 gcgccggagc ataaccagat tgttaaccac ctgatttacc ccattcccga ccccgcgatg
     2161 ccatttttgg gcgtgcatct cacccgcatg atcgacggca gcgtgaccgt cgggccaaac
     2221 gcggtgctgg ctttcaaacg cgaaggctat cgcaagcgcg acttctcatt tagtgacacg
     2281 ctggagattt tgagctcgtc ggggattcgc cgggtgctgc aaaaccatct acgctcagga
     2341 ctgggcgaga tgaaaaactc gctgtgcaaa agcggctacc tgcggctggt gcaaaagtat
     2401 tgtccccggc tttcgttaag cgatctccag ccctggcccg ccggtgtgcg ggcgcaggca
     2461 gtatcgccgg acggcaagct gattgacgat tttctgtttg tcaccacccc acgcacgatc
     2521 cacacctgca atgcgccctc cccggcagcg acatcagcaa ttcctattgg tgcgcatatt
     2581 gtcagcaagg tacaaacgct gttggcaagc cagagtaacc ccggacgcac gctgcgagcg
     2641 gcacgtagtg tggatgcctt acacgccgca tttaatcaat aacctttgaa aacaggacgt
     2701 aacaatgaaa cttaacgaca gtaacttatt ccgccagcag gcgttgatta acggggaatg
     2761 gctggacgcc aacaatggcg ggatcatcga cgtcaccaat ccggcgaacg gcgacaagct
     2821 gggtagcgtg ccgaaaatgg gcgctgatga aacccgcgcc gctatcgacg ccgccaaccg
     2881 cgccctgccc gcctggcgtg cgctcaccgc caaagaacgc gccaacattc tgcgcaactg
     2941 gttcaatttg atgatggagc atcaggacga tttagcgcgc ctgatgaccc tcgaacaggg
     3001 caaaccactg gccgaagcga aaggcgaaat cagctacgct gcctctttta ttgagtggtt
     3061 tgccgaagaa ggcaaacgta tttatggcga taccattccc ggtcatcagg ccgataagcg
     3121 cctgattgtt atcaagcagc cgattggcgt taccgcagct atcacgccgt ggaacttccc
     3181 ggcggcgatg attacccgta aagccggtcc ggcgctggcg gcaggctgca ccatggtgct
     3241 gaagcccgcc agtcagacac cgttctctgc gctggcgctg gcggagctgg cgattcgcgc
     3301 gggcattccg gctggcgtgt ttaacgtagt caccggttcg gcaggcgcgg tcggtaatga
     3361 actgaccagc aacccgctgg tacgcaaact gtcgtttacc ggttcgaccg aaattggccg
     3421 ccagttaatg gaacagtgcg cgaaagacat caaaaaagtg tcgctggagc tgggcggcaa
     3481 cgcgccgttt atcgtctttg acgatgccga tctcgataaa gccgtggaag gcgcgctggc
     3541 ctcgaaattc cgcaacgccg ggcaaacctg cgtctgcgcc aaccgtttat acgtgcagga
     3601 cggcgtgtat gaccgctttg ccgaaaaatt gcagcaggcg gtgagcaaac tgcacatcgg
     3661 cgacgggctg gataaaggcg tcaccatcgg gccgctgatc gatgaaaaag cggtagcaaa
     3721 agtggaagag catattgccg atgcgctgga gaaaggtgcg cgtgtggttt gcggcggtaa
     3781 agcggatgaa cgcggcggca acttcttcca gccgaccatt ctggtggacg tgccggccaa
     3841 cgccaaagtg tcgaaagaag agacgttcgg ccccctcgcc ccgctgttcc gctttaaaga
     3901 tgaagccgat gtgattgcgc aagccaatga caccgagttt ggccttgccg cctatttcta
     3961 cgcccgtgat ttaagccgcg tcttccgcgt gggcgaagcg ctggagtacg gcatcgtcgg
     4021 catcaatacc ggcattattt ccaatgaagt ggccccgttc ggcggcatca aagcctcggg
     4081 tctgggtcgt gaaggttcga agtatggcat cgaagattac ttagaaatca aatatatgtg
     4141 catcggtctt taactggaga atgcgaatga acagcaataa agagttaatg cagcgccgca
     4201 gtcaggcgat tccccgtggc gttgggcaaa ttcacccgat tttcgctgac cgcgcggaaa
     4261 actgccgggt gtgggacgtt gaaggccgtg agtatcttga tttcgcgggc gggattgcgg
     4321 tgctcaatac cgggcacctg catccgaagg tggtggccgc agtggaagcg cagttgaaaa
     4381 aactgtcgca cacctgcttc caggtgctgg cttacgagcc gtatctggag ctgtgcgaga
     4441 ttatgaatca gaaggtgccg ggcgatttcg ccaagaaaac gctgctggtt acgaccggtt
     4501 ccgaagcggt ggaaaacgcg gtaaaaatcg cccgcgccgc caccaaacgt agcggcacca
     4561 tcgcttttag cggcgcgtat cacgggcgca cgcattacac gctggcgctg accggcaagg
     4621 tgaatccgta ctctgcgggc atggggctga tgccgggtca tgtttatcgc gcgctttatc
     4681 cttgcccgct gcacggcata agcgaggatg acgctatcgc cagcatccac cggatcttca
     4741 aaaatgatgc cgcgccggaa gatatcgccg ccatcgtgat tgagccggtt cagggcgaag
     4801 gcggtttcta cgccgcgtcg ccagccttta tgcagcgttt acgcgctctg tgtgacgagc
     4861 acgggatcat gctgattgcc gatgaagtgc agagcggcgc ggggcgtacc ggcacgctgt
     4921 ttgcgatgga gcagatgggc gtggcgccgg atcttaccac ctttgcgaaa tcgatcgcag
     4981 gcggtttccc gctggcgggc gtcaccgggc gcgcggaagt gatggacgct gttgcaccgg
     5041 gcggtctggg cggcacctat gccggtaacc cgattgcctg cgtggctgcg ctggaagtgt
     5101 tgaaggtgtt cgagcatgaa aatctgctgc aaaaagccaa cgatctgggg cagaagctaa
     5161 aagacggatt gctggcgata gccgaaaaac acccggagat cggcgacgta cgcgggctgg
     5221 gagcgatgat tgccatcgag ctgtttgaag atggcgatca cagcaaaccg gacgcaaaac
     5281 tcaccgccga gatcgtggca cgcgcccgcg ataagggcct gattcttctc tcctgcggcc
     5341 cgtattacaa cgtgctgcgc atccttgtac cgctcaccat tgaagacgct cagatccgtc
     5401 agggtctgga gatcatcagc cagtgttttg ctgaggcgaa gcagtagcta tgtccgtttg
     5461 taagagttaa ccgttgctaa gggaatgata acgatgcagt aaaaaataat aacgatgagg
     5521 cgaacgacaa agttaaaaac gactgcggac agtcccctcg cccctctgga gagagggtta
     5581 gggtgagggg taaaacacgc ggctaaaccc tcccctggcc ctctccctaa gggagaggga
     5641 gaaaaccctc ccgctttcgt ttttaagatt ttgttgaccg tctcaattaa acgcgtgacc
     5701 cggcgcggga aatgtcgggg cgctctccca agtgacacac tttcgagagg attcaggatg
     5761 gggcaatcat cgcaaccaca tgagttaggc ggcgggctga aatcacgcca cgtcaccatg
     5821 ttgtctattg ccggtgttat cggcgcaagt ctgtttgtcg gttccagcgt cgccatcgcc
     5881 gaagcgggcc cggcggtatt actggcctat ctgttcgccg ggctactggt ggttatgatt
     5941 atgcggatgt tggcggaaat ggcggttgcc acgcccgata ccggttcgtt ttccacctat
     6001 gccgataaag ccattggtcg ctgggcgggc tataccatcg gctggttgta ctggtggttt
     6061 tgggtactgg ttatcccgct ggaagccaat atcgccgcta tgatcctgca ctcgtgggtt
     6121 ccaggcattc ccatctggtt attttccctc gtcattaccc ttgccttaac tggcagtaat
     6181 ttattaagcg ttaaaaacta cggcgaattt gagttctggc tggcgctgtg caaagtcatc
     6241 gctatcctgg cctttatttt ccttggtgca gtcgcaatta gcggttttta cccttatgcc
     6301 gaagtgagcg ggatctcaag attgtgggat agcggcggct ttatgcccaa cggtttcggt
     6361 gcggtattaa gcgcgatgtt gatcaccatg ttctcgttta tgggcgcaga aattgtcacc
     6421 attgccgccg cggaatccga cacgccggaa aaacatattg tccgcgccac caactcggtt
     6481 atctggcgta tttctatctt ctatttgtgc tctatttttg tcgtcgtggc attaataccg
     6541 tggaatatgc cggggctgaa agccgttgga tcttatcgct cggttctgga attgctcaat
     6601 attccccatg cgaaattaat catggactgt gtgatattac tttccgtaac cagttgcctg
     6661 aactcggccc tgtataccgc ctcaaggatg ctctactcct taagccgtcg cggtgatgct
     6721 cccgcggtaa tgggtaaaat caaccgcagt aaaaccccgt acgtggcggt gttactctcc
     6781 accggcgcgg cattcttaac ggtggtggtg aactattacg cgcctgcgaa ggtatttaaa
     6841 tttctgatcg acagctccgg cgctatcgcc ctgctggttt atttagtcat cgccgtttca
     6901 cagttgcgga tgcgcaaaat tctgcgagcg gaaggaagcg aaattcgctt gcgaatgtgg
     6961 ctttatccct ggctcacctg gctggtcatc ggctttatta cctttgtgtt ggtagtgatg
     7021 ctattccgtc cggcgcaaca gttagaagtg atctccaccg gcttattagc gatagggatt
     7081 atctgtaccg tgccaattat ggctcgctgg aaaaagctgg tattgtggca aaaaacaccc
     7141 gttcataata cgcgctgata atgatcagga gtcacaccat gaccattacg tctctggatg
     7201 gctatcgctg gctgaagaac gatattattc gcggtaattt tcaaccggat gaaaaattac
     7261 ggatgagttt gctgacatcg cgttatgcac ttggcgttgg gccgttgcgg gaagcacttt
     7321 ctcaactggt ggcggaacgg ctggtcacgg tggtgaatca aaaagggtat cgggtggcgt
     7381 ctatgtcaga gcaggagctg ctcgatattt tcgatgcccg cgccaatatg gaagcgatgt
     7441 tagtgagtct ggcgattgcc cgcggtggcg atgagtggga ggcagacgtt ctcgcaaaag
     7501 cgcatctgct cagtaagctg gaagcctgtg acgccagcga gaaaatgctt gatgagtggg
     7561 atctgcgtca tcaggcgttt catacagcaa ttgtggcggg ctgtggttct cactatttgc
     7621 tgcaaatgcg tgaacggttg tttgatctgg cggcgcgcta tcgatttatc tggctgcggc
     7681 gaacggtgct ttcggtagaa atgctggagg ataaacacga tcagcaccag accctgactg
     7741 cggcggtact ggcgcgagat accgcgcgcg ccagtgagtt aatgcgccag catttactga
     7801 cgccaattcc cattatccag caggcgatgg ctggcaatta actactcttc cggaatacgc
     7861 aacacttgcc ccggataaat tttatccggg ctttttagca tcggtttatt cgcttcgaag
     7921 attttattgt acagattagc gttaccgtag acctgtttgg aaatggcact cagagtgtcg
     7981 ccagacttaa cggtataaaa ctggctggca gtggctggtg tcgccgtttt cacctgatca
     8041 tcaacactgg caataccgga aatattcccc accgcaacaa ggattttctc cttcgcctcc
     8101 tgactcaggc cgtcaccagt gaccgtcgct ttgccgtcgg caatttgaat gttcacttta
     8161 tcagcatcag gtataccggt tttgctcaga tgctcctgca ccttcttcgc ctgatcgtct
     8221 ttatcgtgct gacctgtaac cgcgtcccag agtttttctc cggcatcttt cacaaaattg
     8281 aacagaccca tagctacctc attcgttaac ggaaacactc agaaagtgta gcagaggaac
     8341 gcggcgcgtt aacgccgcgg gggtgagcac aggtttaatc gcgggtttgc acccagaacg
     8401 cgtgaatcaa accggggata tacccaagca gtgtcagcag aatattaata atgaacgccc
     8461 aaccgaaccc tttaccgagc agcacgccga gcggcggtag aatgatggtg atgacgattc
     8521 tccagaaacc catatgtact ccctataaga aaattactca ttgtttaaaa agagatttta
     8581 tctcttaagc gtagtaattt tagcggagcc tgccagtttc agcttacgct tttactctca
     8641 tttacgctgc tgaggctggt atatgttgca tttttagttt agagcttact taatttagaa
     8701 aatacttaaa taaatatgac tgaactcgcg caattacagg ccagtgccga acaggcagcg
     8761 gccttattga aagcaatgag ccatcctaaa cgattgctga ttctgtgcat gcttagcggt
     8821 tcccccggca ccagcgcggg agagctgacg cgcattaccg gactgagtgc ctctgcgaca
     8881 tcacagcatc tcgctcgtat gcgggacgaa gggcttatcg acagccaacg ggatgcccaa
     8941 cgcattctat attccattaa aaatgaggcg gtaaatgcca ttatcgccac cctgaaaaat
     9001 gtctattgtc cgtaaggagt cactatggct ttgacaacca tttcgccgca tgatgcacaa
     9061 gaattaatcg cacgcggcgc aaagttaatc gatattcgtg acgctgatga atatcttcgt
     9121 gaacatattc ctgaagcaga tctggctcca ttatccgtgc tggaacagtc aggtcttcca
     9181 cctaaattac gtcgcgagca aattatattc cactgccagg caggtaaacg caccagtaat
     9241 aacgccgata aattagcagc gattgccgcc cccgcagaaa tatttttact cgaagatggg
     9301 attgatggct ggaaaagagc tggattgcca gtagcggtaa ataaatctca acccttgccg
     9361 ttaatgcgcc aggtgcagat cgctgcgggt ggtttaatat taatcggcgt tgtactgggt
     9421 tataccgtaa atagcggttt cttcttatta agtggctttg ttggtgccgg gttactgttt
     9481 gcaggaatca gcggtttttg tggaatggca aggttgttag ataagatgcc gtggaaccaa
     9541 cgagcttgag aagcgacgcc ggacgcg
//
LOCUS       DAZPLI010000150         9501 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZPLI010000150 DAZPLI010000000
VERSION     DAZPLI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.151"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..108
                     /locus_tag="ACHUU3_003450"
     CDS             <1..108
                     /locus_tag="ACHUU3_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR2083434.1"
                     /translation="HRTIIAFAVDDNKVFIAGVYHGGQIYETDFLSSAP"
     gene            complement(149..1231)
                     /locus_tag="ACHUU3_003451"
     CDS             complement(149..1231)
                     /locus_tag="ACHUU3_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFR2083435.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3060)
                     /locus_tag="ACHUU3_003452"
     CDS             complement(1228..3060)
                     /locus_tag="ACHUU3_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR2083436.1"
                     /translation="MARMFDTLGFYIMNVQHFDFFTDNEFSNLERYQTCKAMAKGYAG
                     NYKTGQSRLMHARSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAE
                     RTGELIAQFPAEDAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDF
                     SHASVIDPACGGGAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLV
                     LLESVLMPLCVKVKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKY
                     SRSLFGHANLYGLFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYA
                     LDFVTDRDGVFDDVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKG
                     GATWLLPRVKDDACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPL
                     VWAESITSAGFRFSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAI
                     LPQGFIDETGGVVVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVS
                     AYELNSIPLPSLEQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="ACHUU3_003453"
     CDS             complement(3275..3631)
                     /locus_tag="ACHUU3_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083437.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="ACHUU3_003454"
     CDS             complement(3689..4111)
                     /locus_tag="ACHUU3_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR2083438.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="ACHUU3_003455"
     CDS             complement(4127..4852)
                     /locus_tag="ACHUU3_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFR2083439.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="ACHUU3_003456"
     CDS             complement(4874..5620)
                     /locus_tag="ACHUU3_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR2083440.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="ACHUU3_003457"
     CDS             complement(5627..6691)
                     /locus_tag="ACHUU3_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFR2083441.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="ACHUU3_003458"
     CDS             complement(6763..7188)
                     /locus_tag="ACHUU3_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR2083442.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..7448)
                     /locus_tag="ACHUU3_003459"
     CDS             complement(7185..7448)
                     /locus_tag="ACHUU3_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR2083443.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7560..8060
                     /locus_tag="ACHUU3_003460"
     CDS             7560..8060
                     /locus_tag="ACHUU3_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR2083444.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8080..8274
                     /locus_tag="ACHUU3_003461"
     CDS             8080..8274
                     /locus_tag="ACHUU3_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFR2083445.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8274..8564
                     /locus_tag="ACHUU3_003462"
     CDS             8274..8564
                     /locus_tag="ACHUU3_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR2083446.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8844..8996
                     /locus_tag="ACHUU3_003463"
     CDS             8844..8996
                     /locus_tag="ACHUU3_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR2083447.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            9008..9373
                     /locus_tag="ACHUU3_003464"
     CDS             9008..9373
                     /locus_tag="ACHUU3_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001320327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083448.1"
                     /translation="MEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARKIKTA
                     FVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRRGLDG
                     SYDHDVLQVLRTMVTSLGI"
BASE COUNT         2443 a         2491 c         2017 g         2550 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatt tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ctggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggca ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgtt accaatgcat aattaatagc actgttattt
     7501 ttaaaagtaa atagtgatgc tatttcaaag tgattagcaa tcttattaga attggatgta
     7561 tgaaaagaaa atcattgtca gacaccgacc tcaacgccgc cagaagactg aaagaaatct
     7621 ggatggcaaa gaaatctcag ttgggattga ctcaggaacg tgcggcggaa attatggggt
     7681 tttcaacaca aggggcggta agtcactacc taaatggtca aaccccacta aaccttgagg
     7741 ctgttctaaa gtttgctggc ttactgaaag ttccccctga gtcaatcagg ccagacatgg
     7801 tagatttgct gcaaattgca aggatgtatt gtcgagaacc acagcaagat aacatagtcg
     7861 cattaccagc agacacagaa caaacagagg atgaactacc gtttgccgta gagccgatgg
     7921 aacgagattt aatccatacg ttcagagctt ttccgaaaga agatcaagaa cacatgcttc
     7981 aggagatgaa agataaaaaa gaatcaatgg atcgtacggt tgcgcggtgg ttagctgcac
     8041 aaaaaggccg tcgcgcctaa atcagaggag atatgtaaaa tgagtacagt cctttcccca
     8101 atggtttcag aatttgagac agttgagcaa gaaaatagtt acaacgaatg gctgcgggcc
     8161 aaagtagcag caagcctcgc ggatccccgc ccggcgattc cgcatgatga ggtaatggcg
     8221 gaaatggaac aaattattgc ccaaattgct gctgcaactc acagaggtga ataatgttac
     8281 ccgttttatg gctaccatct gctcgtgatg atttgcgcca gatcgtagcc tacatagcta
     8341 aagaaaatcc cgcggcagca cgcagaatga aaattcgaat tgaaacatcc gttttgccgc
     8401 tcacagagca tccttacctg tatcctccaa gcgagcgagt tcttggtctt cgtgaaattg
     8461 taactcatcc taactacatc atcctttacc gagtaacagc atcaagcata gaagtagtaa
     8521 atattgtgca ttctcgaaga cagtatccag gcaaaaacag ttaattattc ccgccgacag
     8581 aaccacctta gggtggtttt ttcttgcccc aacaaaaaac aatagcgatg ctatttacat
     8641 caaaaaaaat aatgatgcta ttgtttcagg cgtcaaccca ccccgcccca cagaatgcag
     8701 ggcaatactt cgagttacca gacagtggtc aggggttaag tagccagccc gaggcgtaag
     8761 aacatgacgg cagggttcaa tctttaacta tgcagcaggt tttttgttcc gccaccccgg
     8821 cgttaagggg aaatgaggtc agcatggata ctatcgatct tggcaacaac gaatctctgg
     8881 tgtgcggcgt gttccctaac caggatggaa cgtttaccgc catgacgtat accaaaagca
     8941 aaacgtttaa aaccgaatct ggcgcgcgtt gctggttagc cagaaacact gactgatgag
     9001 gctgacgatg gaatttaaag atttaccgtc tgatgtacag aaaacagcag ctcatacatt
     9061 gcattctgtg ctgcgagaaa tcgggaaaga tattgcaagc gagccagcaa aagatctggc
     9121 ccggaaaatc aagaccgctt tcgttgagct ttataatgtt ggcactgact ctgaaactgt
     9181 cgagaccaag accgtaagtt caccaatatt ctcacttgag ccaagcgtat tatcaggtga
     9241 aatatgcacc gagatttcca gcgaactgct tccggtaata cgagaagcaa ttcgccgtcg
     9301 cggtttggat ggaagttatg atcatgacgt tctgcaagtt ctcaggacaa tggtgacttc
     9361 actggggatt tgatccctgc gtcctctaag cttttgatat agcggtcctc caagaagcgg
     9421 taaattttgt cgaaatcatc ttttccctct ggtttattca gaggattaca ggacccgtaa
     9481 gtagcggcat acatttcaac c
//
LOCUS       DAZPLI010000151         9496 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZPLI010000151 DAZPLI010000000
VERSION     DAZPLI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.152"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            122..952
                     /locus_tag="ACHUU3_003465"
     CDS             122..952
                     /locus_tag="ACHUU3_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HFR2083449.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHUU3_003466"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHUU3_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR2083450.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2081..3541)
                     /locus_tag="ACHUU3_003467"
     CDS             complement(2081..3541)
                     /locus_tag="ACHUU3_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR2083451.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHUU3_003468"
     CDS             complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHUU3_003468"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR2083452.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5146..6489
                     /gene="gntP"
                     /locus_tag="ACHUU3_003469"
     CDS             5146..6489
                     /gene="gntP"
                     /locus_tag="ACHUU3_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR2083453.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDTATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGQLELVNSVVGLIIVLIISMVA"
     gene            complement(6653..7555)
                     /gene="fimH"
                     /locus_tag="ACHUU3_003470"
     CDS             complement(6653..7555)
                     /gene="fimH"
                     /locus_tag="ACHUU3_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR2083454.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7575..8078)
                     /gene="fimG"
                     /locus_tag="ACHUU3_003471"
     CDS             complement(7575..8078)
                     /gene="fimG"
                     /locus_tag="ACHUU3_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFR2083455.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8091..8621)
                     /gene="fimF"
                     /locus_tag="ACHUU3_003472"
     CDS             complement(8091..8621)
                     /gene="fimF"
                     /locus_tag="ACHUU3_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFR2083456.1"
                     /translation="MRNKPFYLLCAFLWLAVSRALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8631..>9496)
                     /gene="fimD"
                     /locus_tag="ACHUU3_003473"
     CDS             complement(8631..>9496)
                     /gene="fimD"
                     /locus_tag="ACHUU3_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR2083457.1"
                     /translation="KSYCLNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLA
                     GVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQL
                     YYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYAT
                     EYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPL
                     PFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQ
                     QLLTQLSAECR"
BASE COUNT         2259 a         2484 c         2376 g         2377 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaatttt acggcacccg ttaatctcaa actataccat
     1981 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     2041 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     2101 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     2161 cattctgcgg cagatcatcg gcaaaaatgc cgctcaggcc cagcagcgct ttcacgcggt
     2221 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcaaccacgt
     2281 caatggcatt accctgttca tccacgccct gggtgtagcg catccagcca gccacgccca
     2341 gtgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccgga tccagaagac
     2401 gctgcggtaa cttctggctg ccgtccattg caatctgcca ggtacggtga cgcagagacg
     2461 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtctgta ccttccggca
     2521 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
     2581 gattagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagga
     2641 aagagtggct gccgttcagc atacgcagtt tcatcatttc gaacggcaca acgtctgcaa
     2701 cgaactgtgc gcccacttta tcccagtccg gacgaccatt aacgaagtta tcttcaatca
     2761 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
     2821 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
     2881 ggaaggtgac gttctcttca atccatgccg ccagctgcgg atcacgtgcc tgagccagcc
     2941 ccagaaccgc gaccttcgcc acatgaccgt tttcacgcac gttgtcgcag gacatcaccg
     3001 taaacgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     3061 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     3121 gctgaccgct tgccgcatcg gcgcaataac ctttctctgt gaccgtcagg gagacaatcg
     3181 ccgtctgcgg acgcgccatc gcgttgagaa taccttcaca accgtcgatt tccggatgca
     3241 gggcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcca
     3301 cggtgtacag cagttgctgt tttttcaggt tttcaatcag cacacggtcg ttgcctggca
     3361 tcaggttaac ttcgcagata ccccagtcgc tgtcggtact ttccagcaga tgatgggtgt
     3421 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     3481 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     3541 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
     3601 gcgcagccat gccggtgggt atcaacggct aaagaaagcg cgctggatcg ccagttcgac
     3661 accgcgaact tcagccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
     3721 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     3781 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     3841 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     3901 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     3961 atcgttgtca gcacgcacgc cgtaggaacc tgtgcacatg gtgaaaccgt tcgccatgct
     4021 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
     4081 gaggatcggg cgcggcggat cgtccgggtg aacagccata cggacgccaa cttcttcggc
     4141 aaccggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
     4201 atctttatac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
     4261 cggcagaccg gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
     4321 gaagcgttca gcggcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     4381 cttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     4441 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     4501 gtagcacacg gtgcgaatgc cgcactgcgc caggttgcgc agagtctgct gatagttagc
     4561 gatccactgt tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gcacgctttc
     4621 tacgacagac cataccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     4681 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     4741 cgcctgacgg acatcagcta gagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
     4801 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgt ggagccccgg
     4861 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
     4921 tggcgtataa gttctacctt ttggtaagac atgtcaaccc gaaatctgaa attggttaac
     4981 cacatcgcaa gaatttcact ctcacggttg ttgctgcaaa ttgcagcaaa gggtaatcga
     5041 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
     5101 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
     5161 attctctggg tggtattcgg cattggcctg atgctggtac tgaatttgaa gttcaaaatc
     5221 aattcaatgg tggctttgct agtggcggcg ctgtccgtcg ggatgctggc gggcatggat
     5281 ttgatgtcgc tgctgcacac aatgaaagcg ggcttcggca acacgctggg ggaactggct
     5341 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggcgcac
     5401 cagatagcgc atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtaatt
     5461 atcatcggcc tgattttcgg tctggcgatg ttctatgaag tggcctttat catgttagcg
     5521 ccgctggtta ttgtcattgc cgccgaagct aaaatcccgt ttctgaaact ggcgatcccg
     5581 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
     5641 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
     5701 atcccaagtg tcatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
     5761 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
     5821 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
     5881 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat cggttcctcg
     5941 ccgattgcaa tgtttattgc gatggtggtt gcattcgtac tcttcggcac cgcgcgcggg
     6001 cacgatatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagcat tgcaatggtg
     6061 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggt
     6121 gacaccatcg gcatgttgat gtcccacggc aatatctcgc cctacatcat ggcatggctg
     6181 atcaccgtac tgattcgtct ggcgacgggt cagggtgtgg tttcagcgat gaccgccgcc
     6241 gggattatca gtgctgcaat ccttgataca gcaactggtc agctggttgg cgtgaatccg
     6301 gcgctgctgg tgctagcgac ggctgcgggt tccaacaccc tcacccacat taatgatgcc
     6361 tcattctggc tgttcaaagg ttactttgac ctgtcagtaa aagacacgtt gaaaacctgg
     6421 ggtcagctgg agctggtcaa ctccgtggtt gggctgatta ttgtgttgat tatcagcatg
     6481 gtagcgtaaa agagcaccag agcctgccat tggcaggctc tggtcggatt gtcggcaaag
     6541 tgcgctttgt ttattcttat ctggcctaca aagggctaac gtgcgggttt gtagatagcg
     6601 tagcgcatca ggcaatattg cgtacctgca ttagcaatgt cctgtgattt ctttattgat
     6661 aaacaaaagt cacgccaata atcgattgca cattccctgc agtcacctgc cctccagtac
     6721 gtgcgtaatt tgccgttaat cccagactta ccgccgaagt ccctactgct cctaacgata
     6781 ccgtgttatt cgctggaata atcgtaccgt tgcgcgtcaa ctgtacgccg acgccctgcg
     6841 ctggtgaaaa cgaggcggta ttggtgaaaa tcgaattgcc cgcatctgcg gttgtgccgg
     6901 agaggtaata tcccaggttt tggcttttcg cacaataaac ggtaagagga attggcactg
     6961 aaccagggta gtccggcaga gtaacggtga catcacgagc agaaacatcg cagccgccag
     7021 tgggcaccac cacatcatta ttggcgtaaa tattccacac aaactgaaaa tcatcgctgt
     7081 tatagttgtt ggtctgtcgc aaaataagca cggcaattaa tgaaccagct ttaatcgcca
     7141 ccccgccagc actgctcaca ggcgtcaaat aaagcgccac cggccacggc ttatccgttc
     7201 tcgaattata aacaacccgc ggcgtttcgc tggtggtagg aaatggatag ctactgccac
     7261 tatattttac ggtcccggaa aaattagata acacgccgcc ataagccgag cctcgttgca
     7321 gtgtgacata gtctgtaatg gtttccggat aatcgttatg gcaaaagatt tgcgtcgaaa
     7381 gatccacgac caggttttgc cccacattca cggcaggcgc aaggtttaca taaacattag
     7441 cgctgccacc gccaataggg atagcggtgc cattggcggt tttacaggcg aatgaccagg
     7501 catttaccga ccagcccatc agcagtacag caaacagggt aataactcgt ttcattacaa
     7561 tcatctcttc gggttcagct gtaggtatag gtgatgctaa tcactgcctg aatggttccc
     7621 tgagtggctc cgccatttac cgtcaatgct ctgacctgta acgggaagtg cgctgattgt
     7681 gaggaatcat ccacctgaac tgttttggtt gcgccactat tcaatgtgtt gccactgtca
     7741 tcctgtagct ctaactggat gttttgcgcg gtcccctggt ttttataata tccggtactg
     7801 tcggctgccc cgctgaagct ggcagtgacc ctcgacgttc ccaccggaca attagtcaac
     7861 tcaagcgcaa catcatgcca ggccgatgcc gccccggcag acataagact gaaagaataa
     7921 agatcgccga gatctaccgt ggcattggtg gtggaaaccg tgcacggttt ggcgacgacc
     7981 ttaccgttca ccgtgatggt gacatcggct gcctgtatcg tcgcacttgc gagcgccaac
     8041 attgccgcca ataaataccc acgtttgcgc catttcatga gcatctccag ttactgatat
     8101 tcaagagtga aggtagccgt ggcattgata tgccccgcag tgacaggcac ctgtgtcgcc
     8161 attagccggg cgtaaaaatt cagcgtattt ggtttacccg gcgtcagggt cgtccacgaa
     8221 agcgcggacg atggggcatt aaggggtatc tgattttgtt gctcattcag aagctgtatt
     8281 cccagtcccg cagccgctga caccgtattt tcaagtgcaa gcaggttggc attgtggcta
     8341 tctgcaacac cggtaaaccc aacttttacg gcagaaacgg cattaccaca gggtgacagc
     8401 aaaatacgaa atggaacgac aggagtcgtc gcgccaatgt tgttaaattg cttcgccgcg
     8461 ttttccatca gatcaacagt aaaattggtt gattcagcgg ccacactaca gccgttatct
     8521 ctgacatagc cgcggatagt aatcgtgcta tccgcagcca aagcgcgact caccgccaac
     8581 cacaaaaaag cgcacagaag ataaaaaggt ttgtttctca tcacgccccc ttaacgacat
     8641 tcagctgata gctgggttaa taactgctgc tgactctctg gtggcagttg ataattggcg
     8701 acacagtgag cattttcctc ttctccccat ttcacctgaa cttttcccgc taaaggcatt
     8761 ccgctgaggt aaacctgacc attatccgca acaatgccgc tactctggct actctctgat
     8821 gtcaccatcg ccccaaacgg cagcggctta ttattgtggg ttagcgtcat gagcagtttt
     8881 atcccaacgc gcgctttaaa ctctgctcgc acgatcgccc cacgagtggg aacaacgtta
     8941 gcgaccgcgt tatctaaatc gacgttatca gccagggtat tggtatccag cgctactctg
     9001 ttttcccgat attcagtggc ataaggcagc acggcataac cgcgccagtc ggtacgcacc
     9061 cccgtctggt tttcgacttt tgcatctttt gcgccaggcg ctttaacaag caccaccgta
     9121 tcgtttaacg gctgccccag cgttacgcca ttggcatgag ccagtacccc accgctgact
     9181 ccgtaataga gctgcttaat atcatcgcta tggctgtaac cgatgttggc attgccgtaa
     9241 ccaccgcgat aattcagcgt ggcgtagcct gtacttccgc tattaccatc acctcccccg
     9301 gcgtaaccgg tttgcacgct atagctgagg ttgttgtctt ccagcaacgt accgtataca
     9361 ccggccagat tggtcatccg accgttgaga tcgtgtgaca tgctgtaact ggcactggca
     9421 tgtcgccact gagatttact gtcagaacgc agccagtggc tgaaaggaat attgacgtta
     9481 aggcagtatg actttc
//
LOCUS       DAZPLI010000152         9476 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZPLI010000152 DAZPLI010000000
VERSION     DAZPLI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.153"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..173
                     /locus_tag="ACHUU3_003474"
     CDS             <1..173
                     /locus_tag="ACHUU3_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR2083458.1"
                     /translation="RNRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRF
                     GMTPGEYRRKFR"
     gene            complement(345..674)
                     /locus_tag="ACHUU3_003475"
     CDS             complement(345..674)
                     /locus_tag="ACHUU3_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR2083459.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(679..864)
                     /locus_tag="ACHUU3_003476"
     CDS             complement(679..864)
                     /locus_tag="ACHUU3_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR2083460.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(861..3500)
                     /locus_tag="ACHUU3_003477"
     CDS             complement(861..3500)
                     /locus_tag="ACHUU3_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR2083461.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3708..4697
                     /gene="ldhA"
                     /locus_tag="ACHUU3_003478"
     CDS             3708..4697
                     /gene="ldhA"
                     /locus_tag="ACHUU3_003478"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR2083462.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4808..5230
                     /gene="hslJ"
                     /locus_tag="ACHUU3_003479"
     CDS             4808..5230
                     /gene="hslJ"
                     /locus_tag="ACHUU3_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR2083463.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5227..5493)
                     /locus_tag="ACHUU3_003480"
     CDS             complement(5227..5493)
                     /locus_tag="ACHUU3_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR2083464.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5767..9291
                     /gene="nifJ"
                     /locus_tag="ACHUU3_003481"
     CDS             5767..9291
                     /gene="nifJ"
                     /locus_tag="ACHUU3_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR2083465.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2365 a         2467 c         2312 g         2332 t
ORIGIN      
        1 ttcgtaaccg tcgtctcgat ttttgtgcag atgcgattcg ccatgccgca gatgatgaaa
       61 aactggcagg catcggcttt cattggggat tttctgacca gagtcatttt tcaacggtat
      121 ttaagcaacg ctttgggatg acgccaggcg agtatcgacg taaattccgt taactaattc
      181 gcgttgtgtg tactgcaacg ccaattagtt aaaacagata tttgactatg catttttcag
      241 ggcaaaaggc aatttgacag gagtttttga atattcacct tgtttcagga aaatagatca
      301 atcgctttct cctgtagcgt tatcccgata ttgcggtttt gctattaaaa ttttcgcacc
      361 catttaccgt ttatcccttg tacatattcc cccggtttgg cccgagctac gagcttttgc
      421 ccggcgagtt tcgcgatatc atctaccgac acattatttt gctttgccag ttgttggtaa
      481 ctcgctttac gttcggcatt aatgtcggtt actaatttct ctgtttcagc atccgttttc
      541 agcgcaacca gataaccata aaatgtttca cctacccgcc cctgagttct ggcttcatcc
      601 agagtcaatg ccattacatt gctgcttacc agcccaacaa gaaacgcaca aagaagtaat
      661 gttttcttca tcatcgcctc agaaaagatc gctacgagtt tcaagcagct cttcgacatc
      721 tttgtctgcc ttgatgatga tctcatgctc aattttaacg ttcatattga tagtgatcgg
      781 ttccttgggc gcagcgactt caatacgcgg cgtacagcca accagcataa aagatgacgt
      841 caacgcagcc agtaaaattt tcattgtttt tcctcacact cttttccttg cggacagtca
      901 tttcccggca gacgtgcgtt ctgctccaac catgcctgga gattatcgcc aaagcgtaaa
      961 ctgcgccaca gcgtaaaaat attctcttca tgatggtaat taaggtttac cgtaccgctt
     1021 ttaccatcaa cgcgactggt acctgtaatg ttggcctgca tggttaataa accgagatta
     1081 tctaagttaa tttttgtcga tgaccggcta atttccatat agcgcaacca gttaattgct
     1141 gaacccgcag tcatattgtc tttcaccacc gcatctgcgg tgtctttatc cagccgcaag
     1201 gtcattggcc cgctatttgc cagccaacca tctttcacta tccatttttc gttgtttagc
     1261 cataacggca atgcaccact aaatgccccg gacatggcga attgtttcgg attgacggcg
     1321 ctaaccagtt cgctggatga gagattattc agccgcaaca gcgccgggtc atgttgcggc
     1381 atacgtaatt gtttcatcag tacgttaccg cctaacacat cgacgctaac atcagtcaac
     1441 agcaagggtt cttcttcagt ccacggataa cgcccttgca aatcagccgt aatatttttc
     1501 gctgtcacca gattaatcac ttcggcaatt cgcaacgtaa cggggccgcg agtccccaga
     1561 tgccaggctc catcggcaaa acggaaaggc aggacaaaat cgacaccgtt aacctgatta
     1621 tctggcatcc aggcactacc gcctttcaac acgccgtgtc ctcccgcgcg gaatccttgt
     1681 tcaggcgcag cagaaaatgc aacctgagca tacagttccc catcgcgtaa gttcatcttc
     1741 cagtcgggtg gcaccagcgg ctggaataca gtcagtgatt gttttggcca ccaggcgtta
     1801 ccgcgcagac gaataccgtc ccagcgacca tttacccgaa ccgggccaat ctcaccagca
     1861 tgtaaatcgc ctttaaagag gaaatacgtc ggatcgcgcc catcgacgct aaattttaag
     1921 gttgatggcg gtaacacact gccgccagtg aacagcgttt gcccggcgtc cagtgacagc
     1981 gcgccgctga aggagggatg ctgtgcgtca cgtacccaga cgacgggctt gtcgagaatt
     2041 aatcgcggct tttctaccgt catcgtaccg tattgtaact ggtcgaaacc ggtggagagg
     2101 tcggtcagcg taatcgtgct gtcatgccat tcaccttttc ctgcgacatc ccaggtggca
     2161 tttatcggtg taaaactgcc ttttccccag tagcgccact gccagcggcc cgcgtcaggg
     2221 agaaaatcat tcgccagccc atccatatgc agcacgaaat cgcccagttc atattcatgc
     2281 gcctgcaaaa tggcctgcaa acgtccgtca acaccacgtt gggtgacttt tacacccgct
     2341 aaaggccagc ggatttcatc gatgtccagc gaatcgatga ctcttccctt cgaacgtaat
     2401 aacgcgcctg gctcaaaggc cagcgttggg tcagtcagac ttccacttag ctgcgcgggt
     2461 aagcgggcat ataaaatgag gtccgcctgt ttcgcttcac cggtcagctg cagaggcagc
     2521 tggctgttat ccatgcttaa ttttcctggg ccaaaattta gcaccgcgtt gcctttaccc
     2581 gcttgcccct gggtcagcac actcagtcgt ccgctgatca gagcgttctc aagccctgcc
     2641 tgccagttgt cgactttgac acccagtcgg ccactcaaag gaaaacctgc atacggccaa
     2701 ctccagcgac catcgcttac ggtcaattgt tgacgagtaa tttgccacgg caaatcgagc
     2761 aacggatcgc cgttatcccg tgccagcaca atcaattgcc cgctattttc ctgccagtcc
     2821 agctctgcat ccaccagtga cggttcctgc ggcaagttta acgtcgcagt agcatgccca
     2881 cttacaggaa gtccatccgg cacgagcggc atagtaaatt cccccaccag tttaaccggc
     2941 ggctgatttt caaacgcgat gacatccagt tcgctgactg taagttgttg ccctttcagc
     3001 tggccttgaa atttaacttt ttcgccctga taacgcagtt gctgaatatc agaggttaat
     3061 gcgagagaga gttttccctg ccattcctgc cagggagaaa aaatcagttt atcgatattg
     3121 atccaggtgt taggcagcat ggactgccac tgcgcgaggg tttttggagc agctggcgat
     3181 tgctccgtct gcggcaattt cgccagacaa gcagaatcaa gttctaccat gccgacgttc
     3241 aataaccagc ggctgggatg tgaaaggctg gcgttggtga tatgcgcaag ctgacaatct
     3301 cccaccagat aacggagatc gggaatgatt aaacctttac gcgtaatgcg tgggctttca
     3361 tctaatgcaa tacgtgtccc gagcggtagc cagatgcccg ccagcgtggg aacccacagc
     3421 ccgagcgtca tcagcagcgt caacggcaca agaataatca gtaataacag cgcgagaacg
     3481 gctttatatt tacccagcat gggtagttaa tatcctgatt tagcgaaaaa ttaagcattc
     3541 aatacgggta ttgtggcatg tttaaccgtt cagttgaagg ttgcgcctac actaagcatt
     3601 gttgttgatg aatttttcaa tatcgccata gctttcaatt atatttgaaa ttttgtaaaa
     3661 tatttttagt agcttaaatg tgattcaaca tcactggaga aagtcttatg aaactcgccg
     3721 tttatagcac aaaacagtac gacaagaagt acctgcaaca ggtgaacgag tcctttggct
     3781 ttgagctgga attttttgac tttctgctga cggaaaaaac cgctaaaact gccaatggct
     3841 gcgaagcggt atgtattttc gtaaacgatg acggcagccg cccggtgctg gaagagctga
     3901 aaaagcacgg cgttaaatat atcgccctgc gctgtgccgg tttcaataac gtcgaccttg
     3961 acgcggcaaa agaactgggg ctgaaagtag tccgtgttcc agcctatgat ccagaggccg
     4021 ttgctgaaca cgccatcggt atgatgatga cgctgaaccg ccgtattcac cgcgcgtatc
     4081 agcgtacccg tgacgctaac ttctctctgg aaggtctgac cggctttact atgtatggca
     4141 aaacggcagg cgttatcggt accggtaaaa tcggtgtggc gatgctgcgc attctgaaag
     4201 gttttggtat gcgtctgctg gcgttcgatc cgtatccaag tgcagcggcg ctggaactcg
     4261 gtgtggagta tgtcgatctg ccaaccctgt tctctgaatc agacgttatc tctctgcact
     4321 gcccgctgac accggaaaac taccatctgt tgaacgaagc cgccttcgat cagatgaaaa
     4381 atggcgtgat gatcgtcaat accagtcgcg gtgcattgat tgattctcag gcagcaattg
     4441 aagcgctgaa aaatcagaaa attgggtcgt tgggtatgga cgtctatgag aacgaacgcg
     4501 atctattctt tgaagataaa tccaacgacg tgatccagga tgacgtattc cgtcgcctgt
     4561 ctgcctgcca caacgtgctg tttaccgggc accaggcatt cctgacagca gaagctctga
     4621 ccagtatttc tcagactacg ctgcaaaact taagcaatct ggaaaaaggc gaaacctgcc
     4681 cgaacgaact ggtttaatct tgccgctccc ctgcattcca ggggagctta ttcagataat
     4741 ccccaaagac ctttcatctt ctattcttaa aatagccctg agtcagaaac tgtaattgag
     4801 aaccacaatg aagaaagtag ccgcgtttgt tgcgctaagc ctgctgatgg cgggatgtgt
     4861 aagtaatgac aaaattgctg ttacgccaga acagctacag catcatcgct ttgtgctgga
     4921 aagcgtaaac ggtaagcccg tgaccagcga taaaaatccg ccagaaatca gctttggtga
     4981 aaaaatgatg atttccggca gcatgtgtaa ccgctttagc ggtgaaggca aactgtctaa
     5041 tggtgaactg acagccaaag ggctggcaat gacccgtatg atgtgcgcta acccgcagct
     5101 taatgaactc gataacacca ttagcgaaat gctgaaagaa ggtgcacaag tggatctgac
     5161 cgcgaaccag ttaacgctgg cgaccgcgaa acagacatta acttataagc tggcggattt
     5221 aatgaattaa tagctgccac agctcccggc ggcaagtgac tgttcgctac agcgtttgcc
     5281 gttgggtaat gcacacatcc caatcgccgt accatccagt tgacgggcaa cagaaagcga
     5341 accgccgatc attgcacaat ttgcttctcc actactggac atcgacgctt ttaaacctgg
     5401 cgctacgtgc gccgcagtcg cctgctgaac aggttcacta ctacacgccg acaacaataa
     5461 agcggcacac cctacccaaa acgctgctcg catctcttct tcctctgatc ttcaagccaa
     5521 acgacaccgc cataaataat aggcagcaca gagggcggcg tcgagagctg tcctgcgcgt
     5581 tgccccgcat ttttactttt ttatggctat ttttttgccc tctgtttgat caaaacattc
     5641 attacgctga tgtgggggac acaaaagcga aaatgcagaa gaaagccatt tgctaaaatt
     5701 gaaggattac tactgggcgc gcagcaattt cgtgcgcccc tcatttgcgc aatgtaaggg
     5761 tgtcatatga ttactattga tggtaatggc gcggttgctt cggtggcgtt tcgcaccagt
     5821 gaagttatcg ccatctaccc tattaccccc agttccacga tggcagaaca ggctgatgcc
     5881 tgggccggaa acggcttgaa gaacgtttgg ggagacacac catgcgtggt tgaaatgcag
     5941 tcggaagcgg gtgctatcgc taccgtgcat ggcgctttgc agacgggtgc cctttcaaca
     6001 tcgtttacgt catcgcaggg tttgctgctg atgatcccga cactgtacaa actggcaggc
     6061 gaactcacac cgtttgtcct gcatgtagcg gcacgtaccg ttgccacaca tgcactctct
     6121 atttttggcg atcattccga cgtcatggcg gtgcgccaga cgggttgcgc gatgttgtgt
     6181 gcggcaaacg tccaggaagc gcaagacttt gctctcattt cgcacatcgc gacgctgaaa
     6241 agccgcgtgc catttattca tttctttgat ggtttccgca cgtcccacga aatcaacaaa
     6301 attgtcccgc tggccgatga cacgattctt gatctcatgc cgcaggccga aattgatgct
     6361 catcgcgccc gggcgctcaa cccggaacat ccggtgatcc gtggtacgtc cgccaatcct
     6421 gacacttatt tccagtctcg cgaagccacc aacccctggt acaacgcagt ctatgaccat
     6481 gttgaacagg cgatgaatga tttctctgcc gcgacaggtc gtcagtatca gccgtttgaa
     6541 tattacgggc atccgcaagc tgaacgggtg attatcctga tgggctcagc cattggcacc
     6601 tgtgaagaag tggttgatga attgctaacc cgtggcgaaa aagtcggcgt gctgaaagtt
     6661 cgcctgtacc gccccttctc agccaaacac ttgctacaaa ctctaccggg atccgtacgc
     6721 agcgtggcgg tactggacag aaccaaagaa cccggtgccc aggcagaacc gctctatctg
     6781 gatgtgatga ccgcactggc agaagccttt aataatggca agcgcgaaac tctgccccgt
     6841 gtcattggtg ggcgctatgg tctttcatcc aaagaatttg gcccggactg tgtactggcg
     6901 gtatttgccg agctcaacgc ggctaaaccg aaagcgcgct ttacggttgg tatttacgat
     6961 gatgtaacca atctgtccct gccgttgccg gaaaacaccc agccaaactc ggcgaaactt
     7021 gaagccttgt tttatggcct tggtagtgat ggcagcgttt ccgcgaccaa aaacaatatc
     7081 aagattatcg gtaattccac gccgtggtac gcacagggct attttgttta cgactccaaa
     7141 aaagcgggcg gcctgacggt ttctcacctt cgcgtgagcg aacagccgat tcgttccgct
     7201 tatctcattt cccaggctga ttttgttggc tgccaccagt tgcagtttat cgataaatat
     7261 cagatggctg agcgtttaaa acctggcggc attttcctgc tcaacacgcc gtacagcgca
     7321 gatgaagtgt ggtcgcgctt gccgcaagaa gttcaggccg tgttaaacca gaaaaaagcg
     7381 cgcttctatg tgattaacgc ggcgaaaatc gcccgcaaat gtggcctggc ggcccgtatt
     7441 aataccgtca tgcagatggc ttttttccat ctgacgcaaa ttctgcctgg cgatagcgcc
     7501 ctcgcagaat tgcagggtgc gattgccaaa agttacagta gcaaaggcca ggatctggtg
     7561 gaacgcaact ggcaggctct ggcgctggcg cgtgaatccg tagaagaagt tccgttgcaa
     7621 ccggtaaatc cgcacagcgc caatcgaccg ccagtggttt ccgatgccgc ccctgatttc
     7681 gtgaaaaccg taaccgcagc gatgctcgca gggcttggtg atgccctccc cgtttcggcg
     7741 ctgccgccag acggcacctg gccgatgggc accactcgct gggaaaaacg caatatcgcc
     7801 gaagagatcc ccatctggaa agaggaactc tgtacccaat gtaaccactg cgtcgcagct
     7861 tgcccacact cagctattcg cgcaaaagtg gtaccacccg aagcgatgga aaacgcccct
     7921 gccagcctgc attcgctgga tgtgaaatcg cgtgatatgc gcgggcagaa atatgtcttg
     7981 caggtggctc cggaagattg caccggttgt aacctgtgcg tcgaagtttg cccggcgaaa
     8041 gaccgtcaga atccagagat taaagccatc aatatgatgt cgcgcctgga acatgtcgaa
     8101 gaagagaaaa tcaattacga tttcttcctc aacctgccag aaatcgaccg tagcaaactg
     8161 gaacgtattg atattcgtac atcgcagctg attacaccgc tgtttgaata ttccggtgct
     8221 tgctccggtt gtggcgagac gccgtatatt aaattgctga ctcagctcta tggcgaccgg
     8281 atgttgatcg ctaacgccac cggctgttct tcaatttatg gcggtaacct gccctctaca
     8341 ccgtatacca ccgatgccaa cggtcgtggg ccggcatggg cgaactctct atttgaagat
     8401 aatgccgaat ttggccttgg tttccgcctg acggtcgatc aacaccgtgt ccgcgtgctg
     8461 cgtctgctgg atcaatttgc cgataaaatc ccggcggaat tactgacggc gttgaaatca
     8521 gacgctacgc cagaggttcg tcgagaacag gtcgcagctt tacgccagca actcaacgat
     8581 gttgccgaag cacatgaact gctacgtgat gcagatgcac tggtggaaaa atcaatctgg
     8641 ctgattggtg gtgatggctg ggcttacgat atcggctttg gcggtctgga tcatgtattg
     8701 agtttgacgg aaaacgtcaa cattctggtg ctggatacgc aatgctattc caacaccggt
     8761 ggtcaggcgt cgaaagcgac accgctgggt gcagtaacta aatttggcga gcacggcaaa
     8821 cgtaaagcgc gtaaagatct tggcgtcagt atgatgatgt acggtcatgt ttatgtggcg
     8881 cagatttctc tcggcgcgca gctgaaccag acggtgaaag cgattcagga agcggaagcg
     8941 tatccgggac catcgctgat cattgcttat agcccgtgtg aagagcatgg ttacgatctg
     9001 gcactcagcc atgaccagat gcgccaactc acagctaccg gcttctggcc gctatatcgc
     9061 ttcgatcctc gtcgtgccga tgaaggcaaa ctgccgctgg cgctggattc acgcccgccg
     9121 tcagaagcac tggaagaaac gttacttcac gagcaacgtt tccgtcgtct gaattcgcag
     9181 cagccagaag tggcagaaca gttatggaaa gatgctgcag ctgatttgca aaaacgctat
     9241 gacttcctgg cacaaatggc cggaaaagcg gaaaaaagca acaccgatta aatgctctgg
     9301 ataaggatta cccaattcta aaaaaaagcc cggacgactg ttcgggcttg tctttttata
     9361 tgttggaaaa tcagtggcaa tgcaatggcc caacagaaaa taaaatctgt gacaataaag
     9421 gcatataacc cgcgcagaat aacgtataga aaattaaaag tataattttt attttg
//
LOCUS       DAZPLI010000153         9401 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZPLI010000153 DAZPLI010000000
VERSION     DAZPLI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.154"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            205..1071
                     /gene="folD"
                     /locus_tag="ACHUU3_003482"
     CDS             205..1071
                     /gene="folD"
                     /locus_tag="ACHUU3_003482"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR2083466.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1073..1285
                     /gene="ybcJ"
                     /locus_tag="ACHUU3_003483"
     CDS             1073..1285
                     /gene="ybcJ"
                     /locus_tag="ACHUU3_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR2083467.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1393..1914
                     /locus_tag="ACHUU3_003484"
     CDS             1393..1914
                     /locus_tag="ACHUU3_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR2083468.1"
                     /translation="MPTVITHAAVPLCIGLGLSSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSM
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSCYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="ACHUU3_003485"
     CDS             complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="ACHUU3_003485"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR2083469.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3509..4003
                     /gene="ppiB"
                     /locus_tag="ACHUU3_003486"
     CDS             3509..4003
                     /gene="ppiB"
                     /locus_tag="ACHUU3_003486"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR2083470.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCCEGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4006..4728
                     /gene="lpxH"
                     /locus_tag="ACHUU3_003487"
     CDS             4006..4728
                     /gene="lpxH"
                     /locus_tag="ACHUU3_003487"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR2083471.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4846..5355
                     /gene="purE"
                     /locus_tag="ACHUU3_003488"
     CDS             4846..5355
                     /gene="purE"
                     /locus_tag="ACHUU3_003488"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR2083472.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5352..6419
                     /gene="purK"
                     /locus_tag="ACHUU3_003489"
     CDS             5352..6419
                     /gene="purK"
                     /locus_tag="ACHUU3_003489"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR2083473.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6556..7449)
                     /locus_tag="ACHUU3_003490"
     CDS             complement(6556..7449)
                     /locus_tag="ACHUU3_003490"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR2083474.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7446..8099)
                     /locus_tag="ACHUU3_003491"
     CDS             complement(7446..8099)
                     /locus_tag="ACHUU3_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR2083475.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8110..9369)
                     /locus_tag="ACHUU3_003492"
     CDS             complement(8110..9369)
                     /locus_tag="ACHUU3_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR2083476.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2092 a         2666 c         2493 g         2150 t
ORIGIN      
        1 tagacaagga aataagtcag ttccagattt acaacgccat catggacgaa aaatgaagct
       61 ttcaatctca gcgacggtgc gccccacctt cgcaagaggt cgcttcacgc gataaatctg
      121 aaacgaaacc tgacagcgcg ccccgcttct gacaaaatag gcgcatcccc ttcgacctac
      181 gtaacagatg gaatcctctc tctgatggca gcaaagatta ttgacggtaa aacgattgcg
      241 cagcaggtgc gctctgaagt tgctcaaaaa gttcaggcgc gtattgcagc cggactgcgg
      301 gcaccaggac tggccgttgt gctggtgggt agtaaccctg catcgcaaat ttatgtcgca
      361 agcaaacgca aggcttgtga agaagtcggg ttcgtctccc gctcttatga cctcccggaa
      421 accaccagcg aagcggagct gttggagctt atcgatgcgc tgaatgccga caacaccatc
      481 gatggcattc tggttcaact gccgttaccg gcgggtattg ataacgtcaa agtgctggaa
      541 cgtattcacc cggacaaaga cgtagacggt ttccatcctt ataacgtcgg tcgcctgtgc
      601 cagcgcgcgc cgcgtctgcg cccctgcacc cctcgcggca tcgtcacgct gcttgagcgt
      661 tacaacattg ataccttcgg cctcaacgcc gtggtgattg gcgcatcgaa tatcgtcggt
      721 cgtccaatga gcatggaact gttgctcgca ggttgcacca ctaccgtgac tcaccgcttc
      781 actaaaaatc tgcgtcacca cgtagaaaat gccgatctac tgatcgttgc cgtcggcaag
      841 ccgggcttta ttcctggtga ctggatcaaa gaaggcgcaa ttgtgattga tgtcggcatc
      901 aaccgtctgg aaaatggcaa agttgtgggc gacgtcgtgt ttgaagacgc ggctaaacgc
      961 gcctcataca ttacgcctgt tccgggcggc gttggcccga tgacggttgc cacgctgatt
     1021 gaaaacacgc tacaggcgtg cgttgaatat catgatccac agggtgagta acatggcgac
     1081 attttcttta ggtaaacatc cgcacgttga gctgtgcgac ttgctgaaac tggaaggctg
     1141 gagcgaaagc ggcgcgcagg cgaaaatcgc gattgccgaa ggccaggtga aagccgacgg
     1201 cgcggttgaa acgcgcaaac gctgcaaaat cgtcgccggt cagacagtga gttttgcagg
     1261 tcacagcgta caggttgttg cctgatccct ttcccggcct gtatcaacag gccggttctc
     1321 ttttcagact tttgccctct acgccccgtt catccatgct gatagcatcg acattttgcc
     1381 gaagcgacca tcatgccaac cgttattacg cacgccgccg ttcccctatg cattggttta
     1441 gggttgagtt cgaaagtcat ccctccgcgt ctgttatttg ccggaatcat cctggcgatg
     1501 ctgcccgacg ccgacgtatt gtcgtttaaa tttggcgttg cttacggcaa tgtttttggt
     1561 catcgcgggt ttacccattc gctggtgttt gcgtttgttg tcccgctgtt atgtgtgctg
     1621 attggacgcc gatggttcag ggctgggctg attcgctgct ggctgttttt aaccgtctcg
     1681 ttgctatcgc acagcatgct ggattcggta accactggcg gtaagggtgt tggctggctg
     1741 tggccgtggt cagatgaacg ctttttcgct ccctggcagg tgattaaagt cgcgccgttt
     1801 gcattgtctt gttacaccac gccgtacggg catcaggtga ttatttctga attgatgtgg
     1861 gtgtggttgc cggggatgct actgatggga atgttgtggt ggcgcagacg ataaccggat
     1921 gcgaaaactc gcatccggca atagcgcaat tacttacgac gccaggtggt cccttgcggg
     1981 ccatcttcca gaacgatccc catctcgtta agacggtcac gcgccgcatc tgccgccgcc
     2041 cagtctttcg ctttacgggc atccagacgc tgttgaatta acgcttcaat ctcagccact
     2101 tcgctgtcgt ctgcctgcgc gccgctttgc aggaacgctt ccggttcttg ctccagcagg
     2161 cctaatacag cggaaagttt acgcaggtga gatgccattg cattcgccgc tgccatatct
     2221 tctgctttca gacggtttac ttcacgcgcc atatcaaaca gtacggaata ggcttccggg
     2281 gtgttgaaat cgtcgtccat cgcttcaata aagcgcgctt caaacgcttc gccaccggca
     2341 ggagcaacgg ttttttctgt gccgcgcagg gcagtgtaga gacgctccag cgccgcacgc
     2401 gcctgcttca ggttctcttc gctgtagttc agctggctgc gatagtggcc cgacatcagg
     2461 aagtaacgca cggtttcagc atcgtagtat ttcagcacat cgcgcacggt aaagaagtta
     2521 cccagcgatt tggacatctt ctcgcggtca accatcacca ttccggagtg catccagtag
     2581 ttaacatact gaccatcatg ggcacaggtg gactgcgcga tttcattctc gtggtgcggg
     2641 aacatcaggt cagaaccgcc gccgtggata tcaaagtggt tacccagctg cttgcagttc
     2701 attgccgaac attcaatgtg ccagcctgga cggcccgcgc cccacggaga cggccagctc
     2761 ggttcgccct ctttcgacat cttccacaga acgaagtcca tcgggttgcg tttgtcgtcg
     2821 accacgtcaa cgcgcgcgcc tgcctgcagc tggtcgagat cctgacgcga cagcacacca
     2881 taagtcggat cggtcgggac gtcgaacatc acgtcgccgt tgtccgccgc ataagcgtga
     2941 cctttggcga tcagttgttc agtgagttca ataatttctg cgatatggtg cgtcgcgcgc
     3001 ggctccatat ccgggcgcag aatgttcaaa gcatcaaaat ctttgtgcat ttcggcgatc
     3061 atgcgatcca ccagcgccac aaagctttcg ccattttcat tggcacgttt gatgattttg
     3121 tcgtcgatat cggtaatgtt gcgcacatac ttcagtttat agccgaggaa acgcagatag
     3181 cgcgcaacca cgtcaaaagc aacaaaggta cgcccgtgac cgatatgaca gagatcgtaa
     3241 acggtgattc cacacacata catgccgact tccccggcgt gaataggctt aaattcctct
     3301 ttttggcgtg tcagagtatt gaagattttt agcatcgaag attccgttta gacatgtgtg
     3361 ggtaattgag ttgcgtataa tacccatatt tcccgcgcga atcagcatac attgcgtgat
     3421 gatccgatcg tgcggttatg ctataacacc accctatata tgacccgaac tgggttgaag
     3481 caccaatcaa acggaacagg atgcaaaaat ggttactttc cacaccaatc acggcgatat
     3541 tgtcatcaaa acttttgacg ataaagcacc tgaaacagtt aaaaacttcc tggactactg
     3601 ctgcgaaggt ttttacaaca acaccatttt ccaccgtgtt atcaacggct ttatgattca
     3661 gggcggcggt tttgaaccgg gcatgaaaca aaaagccacc aaagaaccga tcaaaaacga
     3721 agccaacaac ggtctgaaaa atacccgtgg tacgctggca atggcccgta ctcaggctcc
     3781 gcactctgca actgcacagt tcttcatcaa cgtggttgat aacgacttcc tgaacttctc
     3841 tggcgaaagc ctgcaaggtt ggggctactg cgtgtttgct gaagtggttg aaggcatgga
     3901 cgtggtagac aaaatcaaag gtgttgcaac cggtcgtagc ggtatgcacc aggacgtgcc
     3961 aaaagaagac gttatcattg aaagcgtgac cgttagcgag taatcgtggc gacactcttt
     4021 attgcagatc ttcatctctg cgtggaagaa ccggcgatca ccgccggttt tctgcgtttt
     4081 ttagcggggg aagctcgcaa ggccgacgcg ctgtatattc ttggcgatct gtttgaagcg
     4141 tggattggcg acgacgagcc caatccactc catcgccaga tggcggcagc gatcaaagcg
     4201 gtgtccgatt ctggcgttcc ctgttatttc attcatggca accgtgattt tctgctcggc
     4261 aaacgctttg cccgtgaaag tggcatgacg ttattgccgg aagaaaaggt gctcgaactt
     4321 tatggccgcc gggtgttgat tatgcatggc gacacgctgt gcaccgatga cgcgggttat
     4381 caggcttttc gcgccaaagt ccacaaaccc tggctgcaaa tgctattcct cgccctgccg
     4441 ttgtttgtgc gcaaacgcat tgccgcgcga atgcgagcga acagtaaaga agccaacagc
     4501 agtaaatcgc tggcgatcat ggacgttaac caaaacgcgg tagtcagtgc gatggaaaaa
     4561 catcaggtgc aatggctgat ccacggacat acccatcgcc cggcggtaca tgaacttatc
     4621 gccaaccagc aacctgcttt tcgcgtggta ctcggtgcct ggcatacgga aggttcgatg
     4681 gtgaaagtca cggcggatga cgttgagctg attcattttc cgttttaaaa aaaccgcaac
     4741 tttgctgatt tcacagccac gcaaccgttt tccttgctct ctttccgtgc tattctctgt
     4801 gccctctaaa gccgagagtt gtgcaccaca ggagttttaa gacgcatgtc ttcccgcaat
     4861 aatccggcgc gtgtcgccat cgtgatgggg tccaaaagcg actgggctac catgcagttc
     4921 gccgccgaaa tcttcgaaat cctgaatgtc ccgcaccacg ttgaagtggt ttctgctcac
     4981 cgcacccccg ataaactgtt cagcttcgcc gaaagcgccg aagagaacgg ttatcaggtg
     5041 attattgcgg gcgcaggtgg cgcagcgcac ctgccaggca tgattgccgc caaaacgctg
     5101 gtgccggtgc tgggcgtgcc agtacagagc gccgcactga gcggtgtcga tagcctctac
     5161 tccatcgtac aaatgccgcg cggcattccg gtgggtacgc tggcgattgg taaagctggc
     5221 gcggcaaacg cggcattact ggcagcacaa attctcgcga ctcacgataa agagctacac
     5281 cagcgtctga atgactggcg caaagcacaa actgatgaag tgctggaaaa cccggacccg
     5341 cgaggtacgg catgaaacag gtttgcgtcc tcggtaacgg gcagttaggc cgtatgctgc
     5401 gtcaggcagg cgaaccgtta ggcattgctg tctggccagt cgggctggac gctgaaccgg
     5461 cggcggtgcc ttttcaacaa agcgtgatta ccgctgagat cgaacgctgg ccggaaaccg
     5521 cattaacccg cgagctggcg cgccatccgg cctttgtgaa ccgcgatgtg ttcccgatta
     5581 ttgctgaccg tctgactcag aagcagcttt tcgataagct ccacctgccg accgcgccgt
     5641 ggcagttact tgccgatcgc agcgagtggc ctgcggtgtt tgatcgttta ggtgaactgg
     5701 cgattgttaa gcgtcgcact ggtggctatg acggtcgcgg tcaatggcgt ttacgcgcca
     5761 atgaaaccga acagttaccg gcagagtgtt acggcgaatg tattgtcgag cagggcatta
     5821 acttctctgg tgaagtgtcg ctggttggcg cgcgcggctt tgatggcagc accgtgtttt
     5881 atacgctgac gcataacctg catcaggacg gtattttgcg caccagcgtc gcttttccgc
     5941 aggccaacgc acagcagcag gcgcaagccg aagagatgct gtcggcgatt atgcaggagc
     6001 tgggctatgt gggcgtgatg gcgatggagt gttttgtcac cccgcaaggt ctgctgatca
     6061 acgaactggc tccgcgtgtg cataacagcg gtcactggac acaaaatggt gccagcatca
     6121 gccagtttga gctgcatctg cgggcgatta ccgatctgcc gttaccgcaa ccagtagtga
     6181 ataatccgtc ggtgatgatc aatctgattg gtagcgatgt gaattatgac tggctgaaat
     6241 tgccgctggt gcatctgcac tggtacgaca aagaagtccg tccggggcgt aaagtggggc
     6301 atctgaattt gaccgacagc gacacatcgc gtctgactgc gacgctggaa gccttaatcc
     6361 cgctgctgcc gccagagtat gccagcggcg tgatttgggc gcagagtaag ttcagttaag
     6421 caggtgctca tttgattgcc ggatgcggcg taaacgcctt atccggctta ccgctccggt
     6481 acccattgta ggcctgataa gacgcgtcaa gcgtcgcatc aggcatggtg caccgattgc
     6541 cggatgctct catcactaca gcgaaataca ggtccccgct tcgcccgcca gcgtctcttc
     6601 aattcgcgat aacgccccaa tccacgcggg tttaccacgg cttctgacat aaccactcac
     6661 cgccgttacc ttcggcccca tcgaaccatc ggctttggca aatggcgcta actcatccgg
     6721 tgtggcatgg cgaatggcac gttgctgcgg cgttccccag ttttcatata ccgcatcagc
     6781 atcggtgagg atcaccagtc catcggcatt aatctgctcg gcgagcaacg cagcggcgag
     6841 atctttatca atcactgctt cactccctgc cccatcttcc gtcacaggca cacctccgcc
     6901 gccactgcaa atcaccacat gcccctcttt gagcaacaac tcgatggctt cgctgtcgag
     6961 aattttacgc ggttgcggag acgccaccac ccggcgcaaa tatttaccat cacgtttcat
     7021 ctgccagccg taagccgctt ccagtgcctc ttgttcttct ggctgataaa ccggaccaat
     7081 aaatttctct ggctgcaaaa acgccggatc atcaggcgaa acctcaatgc gcgtcagcac
     7141 cgtcgtaaca ggcggcatct gcggctgtgc gctcaagctc tgcgccagca tatagccaat
     7201 catcccctgg ctttccgcca ccagcacatc cagcggatac ggttctacct ctttccacgc
     7261 cagattctgt aacgccagca gccccacctg cggcccgttg ccgtgaacaa tcgccagccg
     7321 ataagaacgg gccaggcgtg ccagcgcggg tacagcactg gcgatattgc gatattgatt
     7381 ttccgccgtc agcgcctcac cgcgctggag taaggcgttg cccccaagag ctacaaccag
     7441 tgttttcatg gttttccttg taataattgt tggccaagcc agaaccccag cagcgtatct
     7501 gcccctgacg tatgccccag cgccagcagc gaatcaatcg caacagcggt acgtttcggg
     7561 caactcagag catggacaaa gtgcaggagt ggcgaggcga aatatccttg cgcggcataa
     7621 cgtaaataac tgacgctcac cgctgtggta acgagttgaa gattgtcgga ataggcaaaa
     7681 aactggcgta actcagcagg cagcggatcg cctgccgcca acgccagtgg cccgaaaagc
     7741 ccggtttcct ccgcgcgttg catccatgca gctgcaagcg gttgaggatg cgcaggcggc
     7801 gtaatacgca gcaaacaata acgctgtggc tgtttaaccg tgaaacgccc gaggcgaatc
     7861 ccttgagcca caacctgtgg tcgttcattg ccgcataatc caccgcataa cgcatcgaat
     7921 tgcgcgcggc gaagcaccca tcctccgggg ccgaaaccac taccctgacg atgcagcgtt
     7981 aacagttcgc cgctttccgt catcaggtta atcgcccgtc gccacactcc cgctaaccgc
     8041 caggactggc gataattcgg tgcactacgg ctggcaagca gaggatggat gatcgtcatt
     8101 gcgcgttcct caaccaatcc ccatgctttc agccagtgct tccagcgcct gttcaaaaca
     8161 cgccagcggt gcccgcacag tgcctgcgcc aatctgcccg atccccgcct ctttatgggc
     8221 gataccggta ttgatgagtg gcgtaatgtc ggtttctacc acgcgacgaa tgtccagtcc
     8281 caggcacgcg ccctgaaaat cccagcctgg gatctgcaac tgcatattgc gctcaaggta
     8341 aatttccgcc atctcttcag ataccgctct ggccgcttcc atgccacccg caccgacaaa
     8401 gcgcgttacg ccaggcgctg cgatcattgc cgcacctccg ataccaaagg tttcggtaat
     8461 cgcgctatcg cccatatccg ggttcgcctg ctcctgcgag aagccggtga aaaacagacc
     8521 ttgcggagtg tttacaggcg cagtaaacca gcgttcgccc agcccgctta cccgaatccc
     8581 gaacatattg ccgttgcggg tcattgccgt gacgatgctg cctgcgcgga tcatcgcgcc
     8641 agcatccatc gccgccttgc agtaagccat cgcgaggttg aggaagaact gatcggtcac
     8701 gctgaggaaa tccatcactt cggcgatgtg ctgtttatca tgatcgaggc gagcaatttg
     8761 tggggccagc gcacgcatta acagtgcaga ggaagcaata ttgcgttgat ggaactcatc
     8821 gcccatcgta atgccctgcg ccatcatcgc cgtgagatcg ataccgcgct ccatgcgccc
     8881 cagcgccgcg cttaatactg gcattaacac atcgcgcatc cagcgatggc gagtcaggac
     8941 atcttcgccg taagcgccaa aacgcatcac tttgccgata ccttcgttga ggttgcagta
     9001 cgcccggttg ccgtcggtca cgttctcgac caccagcatc ggcatactgg cagaagtaat
     9061 accgcccatt ggaccgacgg cattcacatg gtgacaagga atgaagttca cttccccctg
     9121 ctccagtatt gccagcgcct gcgcttcatc tttcgcccag ccttcgaaca gacatgcgcc
     9181 cacgcacgcc cctttcatgg gtccggtcat ctcctgccag cgcattggcg gcccggcgtg
     9241 aagcagtgtt ttgccctcgt ttagttcgct gataagtgaa gaagccggtt gcacatccag
     9301 ccagtgtgga cgagcgcgac gaatttgttc gataaccgca gcattggctt gcgccactga
     9361 tgtaaacatg ggaacccctt attgcaaacg ttctaataaa c
//
LOCUS       DAZPLI010000154         9212 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZPLI010000154 DAZPLI010000000
VERSION     DAZPLI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.155"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            73..732
                     /locus_tag="ACHUU3_003493"
     CDS             73..732
                     /locus_tag="ACHUU3_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFR2083477.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            836..1810
                     /gene="ghrB"
                     /locus_tag="ACHUU3_003494"
     CDS             836..1810
                     /gene="ghrB"
                     /locus_tag="ACHUU3_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR2083478.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1860..2570)
                     /locus_tag="ACHUU3_003495"
     CDS             complement(1860..2570)
                     /locus_tag="ACHUU3_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR2083479.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3004..3294
                     /locus_tag="ACHUU3_003496"
     CDS             3004..3294
                     /locus_tag="ACHUU3_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFR2083480.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3575..3787
                     /gene="cspA"
                     /locus_tag="ACHUU3_003497"
     CDS             3575..3787
                     /gene="cspA"
                     /locus_tag="ACHUU3_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR2083481.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            3927..3998
                     /locus_tag="ACHUU3_003498"
     CDS             3927..3998
                     /locus_tag="ACHUU3_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083482.1"
                     /translation="MRNAVRKAGITSRQRLLLFQIAG"
     gene            complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="ACHUU3_003499"
     CDS             complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="ACHUU3_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR2083483.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="ACHUU3_003500"
     CDS             complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="ACHUU3_003500"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR2083484.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="ACHUU3_003501"
     CDS             complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="ACHUU3_003501"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR2083485.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(7538..7837)
                     /locus_tag="ACHUU3_003502"
     CDS             complement(7538..7837)
                     /locus_tag="ACHUU3_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR2083486.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            8012..9007
                     /gene="wecH"
                     /locus_tag="ACHUU3_003503"
     CDS             8012..9007
                     /gene="wecH"
                     /locus_tag="ACHUU3_003503"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR2083487.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(9049..>9212)
                     /locus_tag="ACHUU3_003504"
     CDS             complement(9049..>9212)
                     /locus_tag="ACHUU3_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR2083488.1"
                     /translation="GLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKET
                     QVTQEEYSE"
BASE COUNT         2286 a         2328 c         2268 g         2330 t
ORIGIN      
        1 tcatgttcac gcggcataat ctcccgccac ggaacccgtg gcaaagaata aaaaggttat
       61 taaggattaa caatgaagaa acgtgtttat cttattgccg ccgtagtgag tggcgctctg
      121 gcggtatctg gctgcacaac taacccttac accggcgaac gcgaagcagg caaatctgct
      181 atcggcgcag gtctgggctc tctcgtgggc gcgggtattg gtgcgctctc ttcttcgaag
      241 aaagatcgcg gtaaaggcgc gctgattggc gcagcagcag gcgcagctct gggcggcggc
      301 gtgggttatt acatggatgt gcaggaagcg aagctgcgtg acaaaatgcg cggcactggt
      361 gttagcgtaa cccgcagcgg ggataacatt atcctcaata tgccgaacaa tgtgaccttc
      421 gacagcagca gcgcgaccct gaaaccggca ggcgctaaca ccctgaccgg cgtggcaatg
      481 gtgctgacag agtatccgaa aacggcggtt aacgtgattg gttataccga cagcacgggg
      541 ggtcacgacc tgaacatgcg tctctcccag cagcgtgcgg attccgttgc cagcgcgttg
      601 atcacccagg gcgtggacgc cagccgcatc cgtactcagg gccttggccc ggctaaccca
      661 atcgccagca acagcaccgc agaaggtaag gcgcaaaacc gccgtgtaga aattacctta
      721 agcccgctgt aatccctttc atgccaggtg atgcagaaat cacctggcat tcttcagttt
      781 tccttcatca tatttcaggc taaggtgatc gccttatcag tgaatggaga gaagcatgaa
      841 gccgtccgtt atcctctaca aagccttacc tgatgattta ctgcaacgcc tgcaagagca
      901 tttcaccgtt caccaggtgg caaacctcag cccacaaacc gtcgaacaaa atgcagcaat
      961 ttttgccgaa gctgaaggtt tactgggttc aaacgagaat gttgatgccg cattgctgga
     1021 aaaaatgccg aaactgcatg ccacatcaac gatctccgtc ggctatgaca attttgatgt
     1081 cgatgcgctt accgcccgaa aaattctgct gatgcacacg ccaaccgtat taacagaaac
     1141 cgtcgccgat acgctgatgg cgctggtgtt gtctaccgct cgtcgggttg tggaggtagc
     1201 agaacgggta aaagcaggcg aatggaccgc gagcataggc ccggactggt acggcactga
     1261 cgttcaccat aaaacactgg gcattgtcgg gatgggacgg atcggtatgg cgctggcaca
     1321 acgtgcgcac tttggcttca acatgcccat cctctataac gcgcgccgcc accataaaga
     1381 agcagaagaa cgcttcaacg cccgctactg cgatttggat acactgttac aagagtcaga
     1441 tttcgtttgc ctgatcctgc cgttaactga tgagacgcat catctgtttg gcgcagaaca
     1501 attcgccaaa atgaaatcct ccgccatttt cattaatgcc ggacgtggcc cggtggttga
     1561 cgaaaatgca ctgatcgcag cattacagaa aggggaaatt cacgccgccg ggcttgatgt
     1621 cttcgaacaa gagccactgt ccgtagattc gccgttgctc tcaatggcca acgtcgtcgc
     1681 agtaccgcat attggatctg ccacccatga gacgcgttat ggcatggccg cctgtgccgt
     1741 ggataattta attgatgcgt tacaaggaaa ggttgagaag aactgtgtga atcctcacgt
     1801 cgcggactaa gccgcgactg cgtgaagtaa agcccgataa tcgctcgggc ttttactctt
     1861 tattgggttg cagtaactgc tgtagtccag gcctgattaa acgcctgatg ttgtgccggt
     1921 aatggcgcaa tcagtttgtt atattcactt gcctgctgtg aagtcgggaa ctggatgcca
     1981 tttgcgacaa agctcacctg agtaccttgc tgagcaatgt agtctccaac catcaccagt
     2041 tgttgcgtga aagtttgtgc cgccggaatc aacggttgca atgcatcagc tggcgtcgtt
     2101 accactttgg tgaatgcctg atcaaatacc ggtttcaggt catcgctctg ttttagcgca
     2161 gagtgcgcag catcggcctg caacttcgca ttttgcagtt gttgcgccag cacacccagc
     2221 gaaccattca tttcacgcag cggaccgctt tgcgtaacgt aatcctgcgg tacgcgaatt
     2281 gcgttaacgc tgtctacgac cgggcgcaga ccagaatcca tcgcctgatt cacctgctga
     2341 gaataaccat acagaatcgc gtaatcagag acaaaaggac caaactgttt tttctgatcg
     2401 gcagtcaggg ttggtagacg ttcaccgcta cgcatcactg tattctgcag gaagtcgata
     2461 aacgctttac gctgatcgcc ttctttatca aaacacccac tcaggctaac taccattaat
     2521 aacgccgcaa gaggcgcaaa ccagcgagag caggactttc ctgtcgccat tttcttactc
     2581 ctttcaccca aaaaaagcgc acaacgacac acgcgtgtct gacgtgacaa ggatagtcca
     2641 ggacaggctt gcaggatacc ctttctacgt aatcttcctg gtaaaatcat ggagaaaatg
     2701 aaacaaggtt aatttgttgt ttcattgtta aaaaatgata gtgccgttgc cttttttgta
     2761 gccagagatt tacgccttcg cagaaggtta gattttcgaa atctttacaa aacaggagtg
     2821 gtaagagata tatcggcatt tatcagatcg ttaggtggct ataagtcacg ctaaatgaca
     2881 ggctgaatcg aatcatagcc agagcatgcc ctgacttcac cccgctgtgt ctgcttttcc
     2941 cgactattct taatgagctt cgatgcaatt cacgatcccg cagtgtgatt tgaggagttt
     3001 tcaatggaat ataaagatcc aatgcatgag ctgttgagca gcctggaaca gattgttttt
     3061 aaagatgaaa cgcagaaaat taccctgacg cacagaacaa cgtcctgtac cgaaattgag
     3121 cagttacgaa aagggacagg gttaaaaatc gatgatttcg cccgggtttt gggcgtatca
     3181 gtcgccatgg taaaggaatg ggaatccaga cgcgtgaagc cttcaagtgc cgaactaaaa
     3241 ttgatgcgtt tgattcaagc caacccggca ttaagtaagc agttgatgga atagactttt
     3301 atccacttta ttgctgttta cggtcctgat gacaggaccg ttttccaacc gattaatcat
     3361 aaatatgaaa aataattgtt gcatcacccg ccaatgcgtg gcttaatgca catcaacggt
     3421 ttgacgtaca gaccattaaa gcagtgtagt aaggcaagtc ccttcaagag ttatcgttga
     3481 tacccctcgt agtgcacatt cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc
     3541 gaaaggcaca cttaattatt aaaggtaata cactatgtcc ggtaaaatga ctggtatcgt
     3601 aaaatggttc aacgctgaca aaggcttcgg cttcatcact cctgacgatg gctctaaaga
     3661 tgtgttcgta cacttctctg ctatccagaa cgatggttac aaatctctgg acgaaggtca
     3721 gaaagtgtcc ttcaccatcg aaagcggcgc taaaggcccg gcagctggta acgtaaccag
     3781 cctgtaatct ctgcttaaaa gcacagaatc taagatccct gccatttggc ggggattttt
     3841 ttatttgttt ttcaggaaat aaataatcga tcgcgtaata aaatttagta ttatttttgt
     3901 gaagaataaa tttgggtgca atgagaatgc gcaacgccgt aagaaaggcg ggaataactt
     3961 cccgccaaag actcttactc tttcaaattg caggctaaaa atgccgccag ctcataactc
     4021 tcctgtttaa tatgcaattc acacagtgaa tctcttatca tccaggtgaa aaataaaagc
     4081 gtgaaacaaa tcactattaa agaaagtaat ctatatttct gcggcattcc cagcctcctg
     4141 tgttgatttc caacgagtaa gaggctaacc ttatggtgtt caggcatagg gtagcctcac
     4201 tttgatttat agtcaggtgg ggcttttctc tgtctgcctt tcggtgaata cctgagacaa
     4261 acagtctcaa gcacccgtgg ctattctagc ttaataagtt tgtttcttct ccttgatata
     4321 atcctaaaaa aacctcataa aattaatata tgagataatc tttattcaac agaagattat
     4381 taaaggttgc tgtattattt agcgataata aaaaaagcct gccagatggc aggcttttta
     4441 ataacggcgt tattactgca acagcgaaat atccgcaacg cgcaggaaca gttcgcgcag
     4501 tttctccagc atggtcagac ggttgagacg caattctttg tcatcaacca tgaccatcac
     4561 tttatcgaag aaagcatcaa ccggctcacg cagctctgcc agttcgacca gcgcatcctg
     4621 gtaacgacct tccgcaaagt acggctccag cttgtcacgt agcaccacaa cctgcatcgc
     4681 cagtttaatt tcttccggct ctttcagggt agaggcattc acgcggtcgc tcagcacttc
     4741 gtcagatttc gccagaatgt tagatacacg tttgttcgcc gccgccagtg cagcagctgc
     4801 atccagggta cggaaatgcg ataccgcttt catacgggca tcgaaatcag ccggacgagt
     4861 cggacgacgc gccagtaccg cctggatggt gtcaacggtg taaccttcgt cctgatacca
     4921 ggcgcggaag cgaccgagca taaagtcgat aacatcatca actacgttgg cgttagtcag
     4981 cttatcgcca tacagacgca ctgcttcttc ggtcagcgtt tgcagatcaa ggttgaggtt
     5041 cttctcaacg ataattcgca gcacgccaag tgcggcacga cgcagcgcaa acgggtcttt
     5101 gtcgcctttc ggatgctgac cgataccgaa gatacccgcc agagtatcca tcttatcagc
     5161 aatcgccagc gcacaagcta ccgggttgga cggcaggtca tcaccggcaa agcgcggctg
     5221 atactgctca ttcagcgcga cggctacgtc ttccgcttcg ccatcgtgac gcgcgtagtg
     5281 catccccata acgccttggg tgtcggtgaa ctcgaagacc atgttggtca tcaggtcaca
     5341 cttagacagc aggcccgcac gggttgcgtg gttaacgtca gcgccaatct gttcagcaat
     5401 ccagccagcc agcgcctgga tgcggtcagt tttgtcacgc agcgtaccca gctgttgctg
     5461 gaacagcacg gtttgcaggc gcggcaggtt atcttccaga cgttttttac ggtcggtatt
     5521 gaagaagaac tcggcatccg ccagacgtgg acgaacgact ttctcgttac cagagataat
     5581 ttgctgcgga tctttcgatt cgatgttggc aacgaagatg aagttcggca gcagtttgcc
     5641 atcgttcgca tacaccggga agtatttctg gtcacctttc atggtgtaaa ccagggcttc
     5701 agccggcacc gcgaggaatt tctcttcgaa ttttgcggtc agaacgaccg gccactccac
     5761 cagcgaagcc acttcttcca gcaggctttc gcttaagtca gcgttaccgc caatcttacg
     5821 cgctgcttct tcggcatcgg ctttaatctt cgctttacgt tcttcgtaat cggcgatgac
     5881 tttcccgcgc tcacgcagaa tttccggata ctgatcggcg ttgtcgatgg tgaattccgg
     5941 ttcgcccata aagcggtggc cgcgaatcac gcgatcggac tgaatgccca gaatggttgc
     6001 cggaatgact ttgtcgccca gcagcagggt cacggtgtgc accggacgca cgaagtgcac
     6061 gtcgcttgcg ccccaacgca tcagtttcgg gatcggcagt ttcgccagcg aagtcgcaac
     6121 catattcggc agtagtgctt cagtgctttc gcccttcaca tgggcgcgat acagcagcca
     6181 ttcgccttta tcggtagtca gacgctcagc ctggtcaacg gtaataccgc aaccacgcgc
     6241 ccaaccttct gccgctttgc tcggtttgcc ttcagcgtcg aacgcctggg caatcgccgg
     6301 gccgcgtttt tcgatttcac gatctggttg cgcttccgcc aggttagcta ctttcaacgc
     6361 cagacgacgc ggagcagcaa accattgaac ggtgccgtgt gcgaggccag cgttatccag
     6421 ctccgcagta aagttcgcag caaaggactc agccaggctg cgcagtgctt ttggtggcag
     6481 ctcttcagtg ccgatttcca ccagaaaagt tttctcagac atagccgcct cttacttatc
     6541 tttgttgcac atcgggaagc cgagggcttc acgggaagcg tagtatgctt ctgccactgc
     6601 tttggtcagg gtgcgaatgc gcagaatgta gcgctgacgc tcagtgacgg agatggcttt
     6661 acgcgcatcc agcaggttga agctgtgggc ggctttcaga atacgctcgt aggctggcag
     6721 cggcagcgga ttttccagcg ccagcagctg ctgcgcttct ttctcgtact gctcgaagca
     6781 ggtgaacagg aaatccacat ccgcgtattc gaagttgtaa gtggactgct ccacttcgtt
     6841 ctgatggaac acgtcgccgt aggtggtttt acccagcggg ccgtcgctcc agaccaggtc
     6901 gtaaacgctg tctacgccct gaatgtacat ggccagacgt tccagaccgt aggtgatctc
     6961 gccggtaacc ggtttacact ccagaccacc aacctgctgg aagtaagtga actgcgtcac
     7021 ttccatgccg tttagccaca cttcccagcc cagtccccag gcacccagag tcgggttttc
     7081 ccagttatct tccacgaaac ggatgtcgtg aatagtcggg tccatgccca gctctttcag
     7141 agaaccgagg tacagctcct gaatattgtc cggcgatggc ttaatgacca cctggaactg
     7201 atagtagtgc tgtaaacggt tggggttttc gccgtagcga ccatcggtcg ggcgacgaga
     7261 aggctgaaca taagcagccg ccatcggttc tggccccagc gcgcgcagac aggtcattgg
     7321 gtgagaggtt cccgcgccga cttccatgtc caatggttga acaatggtgc agccctggcg
     7381 agcccagtaa tcctgtaagg tcaggatcaa gccctggaag gtcctggtat caaacttttg
     7441 catattattt cgtgctggat acgtgtggat ttaaaggaag ggatcagtat acccgctgga
     7501 tggaagatat acagtacgaa acgggaaaaa gcagggctta acgcatggaa agatgcaaaa
     7561 aatggccatc tgcgtcaaaa gaacagacaa actgctcttt acggaaggta taaccgcgca
     7621 tttcatagct tccctggaac tgttcgaagg cggtgacgtc gattttttgc tcgccggtgt
     7681 tataacgttt tgccgcctga tccttgcata acgcttccat attcaacgaa cgcagaggat
     7741 caacctttac ccgttgtgct ttctgcacgg gtgacggtgt agaccaaccc actagcacca
     7801 taagcgccat tgccggaaag aaagcgttca tcatcattaa attaccgtct ggtttgcagg
     7861 tgagtcttat tatttatatc ggtatagtgg ctgatatctt acgaatttcc ttaagcctct
     7921 ataagctgta gtgataaaac tcagattatt ccagtgcgcc aaccaaatga tttatcggtc
     7981 agactttttt taaccagata cagagacaca gatgcagccc aaaatttact ggattgataa
     8041 cctgcgaggg atagcgtgtt taatggtggt gatgattcac accactacct ggtatgtgac
     8101 caatgctcat agtgttagcc ccgtcacatg ggatatcgcc aatgttctga attctgcctc
     8161 tcgtgtcagc gtgccgctat ttttcatgat ttccggctat ctcttttttg gcgaacgcag
     8221 cgcccagccg cgccatttct tgcgtatcgg cttatgtctg attttttata gcgcaatcgc
     8281 actgctctac attgcgctgt ttacctccat caatatggag ttagcgctga aaaacctgct
     8341 gcaaaagcca gtgttttacc acttgtggtt tttcttcgcg attgcggtga tttatctggt
     8401 ttcaccgctg attcaggtga agaacgtcgg cggaaaaatg ttgctggtgc taatggcggt
     8461 gattggcatt atcgctaacc caaacacagt gccgcagaaa attgacggtt ttgaatggct
     8521 gccaattaac ttatatatca atggcgatac tttttactac attctgtatg gcatgttggg
     8581 ccgcgctata gggatgatgg acacacagca taaagcactg tcgtgggtga gcgccgcgct
     8641 gtttgcgacg ggggttttta ttatctctcg cgggacatta tatgaattgc agtggcgcgg
     8701 aaattttgcc gatacctggt atctttactg tgggccgatg gtttttatct gcgcaatcgc
     8761 gctattgact ctggttaaaa acacgctgga tacgcgtacc attcgcggac ttggcttaat
     8821 ctcccgccat tcattgggta tatacggatt ccacgccttg attatccatg cgctgcgcac
     8881 ccggggaatt gagcttaaaa attggccaat actggatatt atttggatct tttgcgcgac
     8941 gttggcagcg agtttgttac tttctatgct ggtacaacga atcgacagaa acagattagt
     9001 gagttaagta aaagcccgtt cacattggac tgaccgggct tacgtgaatt attcgctgta
     9061 ttcttcctgg gtaacctgtg tttcttttgg cgggtttatt ccggcatcac gaatattctt
     9121 ctgcaccgct actgcaccaa acaagcttaa gaagaaagcc agtccataaa aacctttttc
     9181 actgagtaat aatgtcgcat tccacagacc ta
//
LOCUS       DAZPLI010000155         8836 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZPLI010000155 DAZPLI010000000
VERSION     DAZPLI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8836
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.156"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..1782)
                     /gene="atoS"
                     /locus_tag="ACHUU3_003505"
     CDS             complement(<1..1782)
                     /gene="atoS"
                     /locus_tag="ACHUU3_003505"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFR2083489.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATF"
     gene            1949..4798
                     /gene="rcsC"
                     /locus_tag="ACHUU3_003506"
     CDS             1949..4798
                     /gene="rcsC"
                     /locus_tag="ACHUU3_003506"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR2083490.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(4998..5648)
                     /gene="rcsB"
                     /locus_tag="ACHUU3_003507"
     CDS             complement(4998..5648)
                     /gene="rcsB"
                     /locus_tag="ACHUU3_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR2083491.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(5665..8337)
                     /gene="rcsD"
                     /locus_tag="ACHUU3_003508"
     CDS             complement(5665..8337)
                     /gene="rcsD"
                     /locus_tag="ACHUU3_003508"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR2083492.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         2168 a         2049 c         2259 g         2360 t
ORIGIN      
        1 gaaggttgcg ccgtagcccg gcaaactggc gacgcgaata tcaccctgat gggcattaat
       61 gatgcgttga cttaacgcca gaccaagccc ggttcctgag gctttggtgg tgaaaaaggg
      121 atcgaagatc tttttttgca gcgagagatc aatgccacag ccgttgtcct ctatcgaaat
      181 ggcctgttgt gagtcgctgt attgccaggt ttgaatgcga attttccctc gtgcgctgat
      241 agcctggacg gcattgatca ggatattcag tagtacctgt ttgagcagtt cacgatcggc
      301 gttaatcggg cttaattcat tatccagttc gcttatgaag tcgacccgcg cttgtacgcc
      361 ggcggtttgt accagtacca gagtttcttc aaccaatgca ttgaggctga cttgttgcca
      421 ttgactgtgg cgtggacgtg aaaattcgag caattgctga ataactttgt taattgaatc
      481 gatttctttg agtactacgg acagatattc ctgatgtatt gggtcactgg tttgttggcg
      541 caagatctgt acataaccac gaatagccgt taacggatta cgtacttcat gcgcgacgcc
      601 agccatcagc tcacccagtg tggcgaggcg ttctgcttgc gccatgcggc gctgggtttc
      661 tttgcgggca gttaaatcag agaaaatcac caaagcacct atcatttcac cgtgcgtgtt
      721 atgaatacga ctggtagtga cactgagttc aatggtgcgg tcacgacctg gaaaactgat
      781 ctccagcgcc acatgttcgg tgccatgttc cagcgtatcc agtactggac tgtagaactg
      841 agtattgtcg aacaacatgg agtaaggctg ccctaccagt tcatggcgtt gatagccagt
      901 gataacttct gctgctgggt tcatggtggt tacatcaccc tggcggtcaa tggcaatgac
      961 gccatcggca gcgttttcaa taatcagatc gttaagtgtc cgcgtttcac gcagtgcctg
     1021 ggcgaggtta ttaacactct gactgatttg ccccatttca ccgggcaatt gtggtaatcg
     1081 agtgggaata ttttgtgcca gagtcgagag gccatcggtg atgatatcaa tattggcgct
     1141 caggcgacgg gagaaaagga caatcaatag caggcttatc agcaaatcgg cggtgagcac
     1201 aatgataatc ctcacatcca ttttccaggc ctggcggcga atatcttcgg ttaattcatt
     1261 ggcccagata tagccgagga tttcaccatt acgctcaatg gggagcattg aattcaaaat
     1321 atcgccgcgc acctgcctgc ctgaataaac caaaggggta tttgtacgca tgacttcgcg
     1381 accagggtga tctgcggcaa tggtaacgcc gacattattc tgatatagcg ctgaaggcgc
     1441 gtaggtgatt atcgcatcca gcattttgtt gtaataacca gcacctatgc cagggaaggc
     1501 gtgagtgata ttttcggtaa tgggggcaag ttctgcattt aatgcgcgga tacgctcctc
     1561 acgtggtaag tcgatgtaga gatcatagcg atcgcctagt gcctgattaa gcaggttgac
     1621 cacggcagaa agttttttct ctttttcaga taagactgct gaacgtcctt ccgtttctac
     1681 gatataacca atagtaagcg ttgggacaat gaccatcagg attgccatca ggatcatttg
     1741 attgcgtaag cggcgtggat aaatccactt catataatgc atgctgaaat ccactagtct
     1801 tgtccggtat atgacgatta tcagaggtta aggtgatgat ttctcggcgg tgtatcatat
     1861 tccagagaag agagaacatt gcggtaacac gcttttaccg ctaccttaac cacactccat
     1921 cggtcacctg aggcggagct tcgccccttt gaaatacctt gcttcttttc gtacaaccct
     1981 gaaagcctcg cgctacatgt tcagagcatt ggcgttagtg ctctggctgt tgattgcttt
     2041 ttcatccgtt ttttacatcg ttaatgcgtt acatcagcga gaatcggaaa ttcgtcagga
     2101 atttaatctg agttccgatc aggctcagcg ctttattcaa cgcacctctg atgtgatgaa
     2161 agagctgaag tacatcgccg aaaatcgctt atcggcagaa aacggtgtgc tttccccgcg
     2221 tggacgagaa acgcaggcgg atgtgcctgc gtttgaaccg ctgtttgccg actccgattg
     2281 ttccgcaatg agtaacacct ggcgaggttc tctggagtca ttggcgtggt ttatgcgcta
     2341 ctggcgcgat aatttttctg cggcttacga tctaaaccgg gtatttttaa tcggcagcga
     2401 taacctctgc atggccaatt tcggtctgcg tgatatgcca gtggaacgcg ataccgcgtt
     2461 gaaagctttg catgaacgca tcaataaata tcgaaatgca ccacaagatg atagcggcag
     2521 taacctctac tggatcagcg aaggtccgcg ccctggcgtc gggtattttt acgcgttgac
     2581 gccagtttat ctggcgaacc ggttgcaggc gcttttgggt gtcgagcaga ccatccggat
     2641 ggagaacttt ttcttaccgg gtacgttgcc gatgggggtt accattcttg atgaaaatgg
     2701 tcataccctg atttcgctta ccggaccaga aagtaaaatt aagggcgatc ctcgctggat
     2761 gcaggaacgc tcctggtttg gctatacgga agggttccgg gagctggtgc tgaagaaaaa
     2821 tctgccaccc tcatcgctaa gcatcgtgta ttcggtgccg gttgataagg tgctggaacg
     2881 cattcgcatg ttgatcctta acgcaatttt gctgaatgtg cttgccggag ctgcattgtt
     2941 tactctcgca cggatgtacg agcgacgtat tttcattccg gcggaaagcg acgccctgcg
     3001 actggaagaa catgagcagt tcaatcgcaa gattgtcgcc tccgcgccag tgggtatctg
     3061 cattttgcgt accgctgatg gcgtcaatat tttaagtaac gaactggcgc atacctatct
     3121 caatatgctt acgcatgagg accgccaacg actgacgcaa attatctgtg ggcagcaggt
     3181 caattttgtt gatgtcctga ccagcaacaa taccaatctg caaatcagct tcgtccattc
     3241 gcgctatcgt aatgaaaacg tggccatttg tgtgctggtg gatgtttctt cgcgcgtgaa
     3301 gatggaagag tcgttgcagg agatggcaca agcagcggaa caggcgagcc agtcaaaatc
     3361 gatgttcctt gccaccgtca gtcatgagct gcgaacgccg ctgtatggca ttatcggtaa
     3421 cctggatctg ttgcaaacca aagagttacc gaaaggcgtc gatcggctgg tgacggcaat
     3481 gaacaactct tccagcctgt tgttgaaaat tatcagcgat attctcgatt tctcgaagat
     3541 tgaatcggaa cagttgaaga tcgaaccgcg tgagttttca ccgcgtgaag tgatgaacca
     3601 catcaccgcc aactatttac cgctggtggt acgcaagcag ttaggcttgt actgctttat
     3661 tgaaccggat gtgccagtgg ccttaaatgg cgacccgatg cgtttacagc aggtcatctc
     3721 caacctgttg agtaacgcca taaaattcac cgataccggc tgtatagttt tgcatgtccg
     3781 cgctgatggc gattatctct ctatccgtgt tcgcgatacc ggcgtgggga ttccggcgaa
     3841 agaagtggtg cgcttgtttg atcccttctt ccaggtcgga acgggcgtac agcgaaactt
     3901 ccaggggacc ggtctgggtc tggcgatttg tgaaaaactg atcagcatga tggacggcga
     3961 tatctcggta gattcagaac cgggaatggg cagccagttt accgtgcgta ttccgttgta
     4021 cggcgctcag tacccgcaga aaaaaggcgt ggaagggttg agtggtaaac gctgctggct
     4081 ggcggtccgc aatgcgtcgc tctgtcagtt cctggaaacc agtttgcagc gcagcggcat
     4141 cgtcgttaca acatacgaag ggcaggaacc gactcccgaa gatgtgttaa ttactgacga
     4201 ggtagtgagt aaaaaatggc agggtagagc ggtagtgacc ttctgtcgtc gccatattgg
     4261 tattccgctg gagaaagcgc caggggagtg ggtacacagt gtggctgccc cgcatgagct
     4321 accggcattg ttggcgcgta tttatttgat cgagatggag agcgacgatc ctgctaacgc
     4381 tctgccgtcg acggacaaag cggtcagcga taatgacgat atgatgattc tggtcgtgga
     4441 tgatcatccg attaaccgac gtctgctggc agatcagttg ggatcgttgg gctatcaatg
     4501 taaaaccgcg aatgatggtg tcgatgcgct taatgtactt agcaagaatc atattgatat
     4561 cgtgcttagc gacgtcaaca tgccaaatat ggatggttac cgcttgacgc aacgcattcg
     4621 tcagttggga ctgacgttgc cggtaatcgg agtaactgct aatgcgttgg ctgaagagaa
     4681 gcagcggtgt ctggagtccg gtatggacag ctgcctgtcg aagccggtaa cgctggatgt
     4741 gataaaacag acgctgacgg tatatgccga gagggtcagg aaatcgcggg aatcgtagga
     4801 cggataaggt gtttatgccg catctggcat tcagtgcaaa tgccagatgc gacgctgacg
     4861 cgtcttatct ggcctacttt gatgtaatct gttgaaataa tgggaatcgt aggacgcata
     4921 aggcgtttac gccgcattcg gcaatcggtg caaatgccag ataagacact aacgcgtctt
     4981 atctggccta caggtgatta gtctttatct gccggactta acgtcactga agagagataa
     5041 ttcagcaggg cgatatcgtt ctcgacaccc agcttcatca tcgcagattt cttctggcta
     5101 ctgatggttt taatactgcg gttcagcttt ttagcgatct cggtcaccag gaagccttcc
     5161 gcaaacaggc gcagaacttc actctctttt ggcgagagac gcttgtcacc gtaaccacca
     5221 gcactgattt tttccaacag gcgagaaacg ctttccgggg taaatttctt ccctttctgc
     5281 agcgcggcga gagctttcgg cagatcggtc ggtgcacctt gtttcagcac gatcccttcg
     5341 atatccagat ccaataccgc actaagaatc gccgggttgt tgttcatagt cagaacaatg
     5401 atcgacaggc ttgggaaatg gcgcttgatg tacttgatta aggtaatgcc atcgccgtac
     5461 ttatcgccag gcatggagag atcggtaatc aacacatgcg catccagttt cggcaggttg
     5521 ttgatcagtg ctgtagagtc ttcaaattcg ccgacaacat tcacccactc aatttgctca
     5581 agtgatttgc gaataccgaa caagactatc ggatggtcat cggcaataat tacgttcata
     5641 ttgttcatgt aataggctac cttgctacag caagctcttg acataactgt caatgtcgct
     5701 gatgtatttt tctattcctg gaacatcctt ctcacgaatc agatgttcca gcgtttcaca
     5761 taactgcttg ccgggtacca gatttagcat ggcaaatacg cctttaagac gatgagccgt
     5821 ttgggctaac gcagcaaagt cactggttgc tgcttcagta tacagcctct taacatcatc
     5881 cggtactgtg tctacaaaga gcgcataata gccgctggca tggagttgcg cattttcatc
     5941 tccgcccaga ggcgattctg tcacctcttc ctgcgccagt tgcacttcaa ttaattgtaa
     6001 gaccgcttcc tgcatagcgt tgctcatatt gaagttgacg cacaattgac caggcccaat
     6061 ttcccgtacg ccagactcat catcgcttaa aagcaagcca gaggcagtaa gattagacgg
     6121 attatccgtt aaaaagatat cataatcttg actaattaat ctttcatcgg gtgtgataca
     6181 ggttgcaccc caattttcta actggcgagt gacaatattc cgaatttctg ccgaagtaac
     6241 atccaccatt acgcagacat catccagtaa acgctcttct tcctcttcaa cttccgggcc
     6301 agcggcgagc attttgatat gcacagagta gcgtgtacca agcccatccc gcgttttgat
     6361 gtttaaatga ccgcccagtt tacgtgccag ttgatcgctc agccagaatg ccagcgggtc
     6421 cgcctttcca taacgatcgt tttgggtctg gttgataaac gggaagtgca aattatccat
     6481 ttcatgaata cttacgcctt ctccggtgtc cagaatgcgg aatgtcaggc ggtcttcaga
     6541 ggactcatcc tgatcaacct caagggtgat ttttcccaat tgcgttgagg tcacggcata
     6601 ttgcatcagt agcagcaaaa tacgtcgtaa ggcatcgcga tcgccgcggc gcatatcgtg
     6661 tgctttcaga tgattgttaa tcagcagttg cagaccttta cgcttgatgg caggcaacac
     6721 tgaaggcaca acttcatcaa ttaaatcctg cacggagaac agcaccgtct cacttttcca
     6781 gctatcgtcc gcaagcatgt tcgctaactg tatttcatcg accagccgca ccagcacatc
     6841 tgcctgattc gccagttgtt tgctttccgg ggcgttgagt ttagccgcgc tctccgccag
     6901 ggactgtgca ggttctttca gcgcatcgcc aatgtttttc ataaaggtca tccggccctg
     6961 ctggtttttc tcatacagac gctgcgcctg cttgagtttc ttgtttacca gcacttcgcg
     7021 atcctgatcg cgaataatga aaatttgtgt gcgcggcgcg acctggctgc ggaacatgcg
     7081 gatctcatac agctcgttat tgatcgtcgc ctgaataatc ccctgatgct gttccgccat
     7141 ggtggtgatg ttttgcagat tcaaatgcgg cagcaaatga tcggcaattt tgttacttat
     7201 gacagtgcgg ttcgattcct gatcgtgaac cagcaggccg agcggcagca gtgagactat
     7261 ctcttcattg attgcccgta aaatgcgcaa ttcgttattg accgccgtgt tgggtagact
     7321 ttctgtactg cgactggaga aatggcggaa tgtggtatag ccaaataacg ccagcgccag
     7381 caaaccgatg ttcagcagca gtggcagcag aatgttttgc aacgtatcca gcaataaggt
     7441 gccataagga acctgccaga ccaggcgcat atcggtagag ttgagtgccg aggagatttc
     7501 aatcttcgta ctgttaaagt ggatactgac gctatccgtt ccttctttct cattatcatt
     7561 gtctcccgtt gccgtcgtgt ctggctcaag gcggaaactg tccagcggca tacccggtgg
     7621 gatcaaatca ttaatcggca gatcaaaagc cacgaccgtt gccagatgtc ccggctggtt
     7681 gaacgtggta cgcaaggtaa agtaatgacc gttctgccag gccaggcggc gcatgttaga
     7741 aaaactttcg cgttcatcga gggcgttggc ctgttgcaac atttctgcac gacgtgaatc
     7801 aacaatgtca ctgacggtcg attctttaaa tccggaggtg agatctttga ggggcagggt
     7861 tgagatcagt accagactgt tatcctgacc attcaggtaa tacatcgacc acggtacatt
     7921 ttctgcaccc cacaatgtat ccagataagt ggacatccgc tgagtcatct caagcgttga
     7981 gttgtcgtga gagccaaaga ttaacgcttc cgttttgcgg cgtggtttct ccagatagta
     8041 gacatcctgt ttcaggcgcg tctcttgtaa accttcgccg gaggaggggg aggtcgtcgc
     8101 ggcaatgttg tcgtagatct gccaggtcac gtaacgccag gtatcgacgc gcttttggat
     8161 tgcgtgggta atgtcgacaa tctggtaact tttatctttc agccaggcgt taacggcgct
     8221 ttgtaccatt acccccatcg tcaccagtaa cacaatgatc aacagtaaaa agaagcgggt
     8281 aatgctcccc ggtaggagtg aaaagcgggt cgtggccgtt gtctctttct gacgcattcg
     8341 tgtttatgac ctgttaaaac ttcgcgaagt gatagggcag tataaagggt acagtgtgaa
     8401 attccagact cttacctgat tgatgattct ctattctccc gtgatattta tcatcctgca
     8461 ttgggggaaa tagatgtaca agcgccattt tgctgtacaa ataacctaca aaaagcccaa
     8521 cgaaattaat tgcaaataaa cgaagattgc tggaaattat gctgatgtta attatttgtg
     8581 aaatagttga caagagttat agtttttctg tggtagcaca gaataatgaa agtgtgtaaa
     8641 gaagggtaaa aaaaaccgga tgcgaggcat ccggttgaaa taggggtaaa cagacattca
     8701 gaaatgaatg acggtaataa ataaagttaa tgatgatagc gggagatatt ctagttgcga
     8761 gtgaaggttt tgttttgaca ttcagtgctg tcaaatactt aagaataagt tattgatttt
     8821 aaccttgaat tattat
//
LOCUS       DAZPLI010000156         8695 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZPLI010000156 DAZPLI010000000
VERSION     DAZPLI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.157"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..134
                     /gene="yhaV"
                     /locus_tag="ACHUU3_003509"
     CDS             <1..134
                     /gene="yhaV"
                     /locus_tag="ACHUU3_003509"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR2083493.1"
                     /translation="NDENTLRTYGKKTNAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(189..998)
                     /gene="agaR"
                     /locus_tag="ACHUU3_003510"
     CDS             complement(189..998)
                     /gene="agaR"
                     /locus_tag="ACHUU3_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFR2083494.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            1247..2527
                     /gene="kbaZ"
                     /locus_tag="ACHUU3_003511"
     CDS             1247..2527
                     /gene="kbaZ"
                     /locus_tag="ACHUU3_003511"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFR2083495.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCNMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGDAHETLSELAVTTPDAARATLEAHRHAFKKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            2550..3023
                     /gene="agaV"
                     /locus_tag="ACHUU3_003512"
     CDS             2550..3023
                     /gene="agaV"
                     /locus_tag="ACHUU3_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFR2083496.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            3034..3813
                     /gene="agaW"
                     /locus_tag="ACHUU3_003513"
     CDS             3034..3813
                     /gene="agaW"
                     /locus_tag="ACHUU3_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFR2083497.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPSAQKEEFEDGI"
     gene            3803..4681
                     /gene="agaE"
                     /locus_tag="ACHUU3_003514"
     CDS             3803..4681
                     /gene="agaE"
                     /locus_tag="ACHUU3_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFR2083498.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            4699..5133
                     /gene="agaF"
                     /locus_tag="ACHUU3_003515"
     CDS             4699..5133
                     /gene="agaF"
                     /locus_tag="ACHUU3_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFR2083499.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTCLVDELGRCHEECPVEEGI"
     gene            5130..6263
                     /gene="nagA"
                     /locus_tag="ACHUU3_003516"
     CDS             5130..6263
                     /gene="nagA"
                     /locus_tag="ACHUU3_003516"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR2083500.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLCITDGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVFDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAAAWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            6614..7768
                     /locus_tag="ACHUU3_003517"
     CDS             6614..7768
                     /locus_tag="ACHUU3_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFR2083501.1"
                     /translation="MPENYTPAAAATDTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYTPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDVIAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            7781..8641
                     /gene="kbaY"
                     /locus_tag="ACHUU3_003518"
     CDS             7781..8641
                     /gene="kbaY"
                     /locus_tag="ACHUU3_003518"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFR2083502.1"
                     /translation="MSIISTKYLLQDAQANDYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KCFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2005 a         2325 c         2314 g         2051 t
ORIGIN      
        1 tgaacgatga aaacactctg cgcacctacg gtaaaaaaac aaatgcctat accgtattca
       61 gcaaaatgtt aaaaagagga catcctcctg ccgactggga aaccctcacc cgagagacag
      121 aagaaaccca ttgatggtgt tcacaatgcc cgccgaacgc attcgtgttg accagcgggc
      181 accctttctc actccccgac cagaatcact tcaaccccag cctttcgcag tccttccaga
      241 ctatccgcag gaatgccttc atcaacaatg atcatgtcga tacgttgagt atcaatgatc
      301 ttatgtaaac tggagcggtt gaacttactg gaatcggtga ccacgatgat ccgttccgca
      361 acttcgcaca tccggcggtt taaacgggct tcatcttcat tatgtgtgct gacgccgcgc
      421 tccagatcga tcgcatctac accaagaaac agcatatcga agtggtaatt ttgcagcgat
      481 tgctcagcct gatcgccgta aaaagattgc gactgacggc gcaaatgccc gccggtcatc
      541 agcagctcaa cgccttcggc ttccagcaac gcattagcca cgttcatacc gttggtcatc
      601 gcaattacgt cagtgtgctt gcgcatcaga cgagcaatct caaaagtggt ggtcccggaa
      661 tcgaggatca cccgatgacc tggctgaatc aactcaacgg cagctttcgc aacgctgcgt
      721 ttcatcgcgg tgttcagtgc gcttttatct tccactgatg gctcgactga cggcgtcgtg
      781 ctatcgcaga tcaacgcgcc accataggca cgcacagcga tcccctgctt ttccagaaac
      841 gccagatcgt tgcggatcgt cacagtagat acgccataca atgccgacag atcgttaacc
      901 tgcacactcc cttgctgtcg caggcgctga atgatctgtt ctcgtcgctc gctggtgcct
      961 gtcactcgct tctcacctga agcgtcggta ttactcatag taagtccttt cgtaaaactt
     1021 tcgtttcatt tcgttttgcc tattaacgcc tttctattaa gcaaatgcaa gcccaccttg
     1081 cccattgacg caagctactc tcgtttcagt aactttcatt atgtttcttt tgtgaatcag
     1141 atcagaaaac tattatcttt cgttttattt ttatctcacc atggcgcagt atcaactgaa
     1201 acaaaacgaa agattaatat cgcagtaatc tgaactggag aggaaagtga aacatctgac
     1261 agaaatggtg agacagcaca aagcgggcaa aacaaatgga atttatgccg tttgttccgc
     1321 acatccgctg gtgctggagg ctgcaatccg ctacgccagt gcaaaccaaa cgccgttact
     1381 gattgaagca acctccaatc aggtagacca gttcggcggt tataccggaa tgacgcccgc
     1441 cgattttcgc ggctttgttt gtcagctcgc cgactcgttg aatttcccgc aggatgcgtt
     1501 gattctgggt ggtgaccatc tggggccaaa ccgctggcaa aacctgccag ccgctcaggc
     1561 aatggccaat gccgatgatt tgattaaaag ctacgttgcg gcaggattca aaaaaatcca
     1621 ccttgattgc aacatgtcct gtcaggacga tccaattccc ttaactgatg acatcgtggc
     1681 tgaacgcgcc gcccgtctgg cgaaagtggc ggaagaaacc tgtcgcgaac actttggcga
     1741 agccgatctg gagtatgtca ttggtaccga agtgccggta cctggcgacg cgcatgaaac
     1801 cttaagcgag ctggcggtca ccacgccgga tgccgcccgc gccacgctgg aagcccatcg
     1861 tcacgccttt aaaaagcagg gtctgaatgc catctggcca cgcatcattg ccctggtggt
     1921 tcaacccggc gtcgaattcg atcacaccaa cgttattgat tatcagcccg ccaaagcgac
     1981 cgccttaagc cagatggtcg aaaactacga aacgctgata ttcgaagcgc actctacaga
     2041 ttatcaaacg ccgcaatcgc tgcgccagct ggtgattgat cactttgcca ttctgaaagt
     2101 tggcccggcg ctgaccttcg ccctgcgtga agccctgttt tctctggcgg cgattgaaga
     2161 agaactggtg ccagcaaaag cctgttccgg tctgcgtcag gtgctggaaa acgtgatgct
     2221 cgaccggccg gaatactggc aaagccacta ccacggtgac ggcaacgcgc gtcgtctggc
     2281 gcgtggttat agctactcgg atcgcgtgcg ctattactgg ccggacagcc agattgatga
     2341 cgctttcgct catctggttc gtaatctggc ggattcacca attccgctac cgctcatcag
     2401 ccagtatctg ccgctgcaat acgtgaaagt tcgctccggc gagctgcaac cgacgccacg
     2461 ggaactcatt atcaaccata ttcaggacat cctggcgcag taccacacag cctgtgaagg
     2521 ccaataagca aaacaaagag gaacacgcta tgccaaatat tgttttaagc cggattgatg
     2581 aacgcttgat tcacggtcag gtcggcgttc agtgggtcgg atttgcgggg gcaaatctgg
     2641 tgctggtagc caacgatgag gttgccgaag atccggtaca acaaaacctg atggaaatgg
     2701 tactggcaga agggatcgcc gtacgtttct ggacgctgca aaaagttatc gacaacattc
     2761 atcgcgccgc cgatcgacag aaaatcctgc tggtttgtaa aacacccgcc gatttcctga
     2821 cgctggtgaa aggtggcgtt ccggtgaatc gcattaacgt tggcaatatg cactacgcca
     2881 atggcaaaca acaaatcgcc aaaacggttt ctgtggatgc gggcgatatc gcagcattta
     2941 acgacctgaa agccgctggg gtggaatgct tcgttcaggg cgtcccgaca gagcctgctg
     3001 tggacctctt taaattactt tgagggattc atcatggaaa tcagtctgtt gcaggcattc
     3061 gcgttgggca ttatcgcctt tatcgctggc ctggatatgt ttaacggcct aacccatatg
     3121 caccgcccgg tggtcctcgg cccgttggtc gggctggtac ttggcgatct gcataccgga
     3181 attttaaccg gcggtacgct ggaactggtg tggatggggc tggccccgct ggcgggcgca
     3241 cagccgccta acgtgattat cggtactatc gtcggcacgg cgtttgccat tactactggc
     3301 gtgaaacccg atgtcgcagt cggtgtcgcc gtacctttcg ctgtcgcagt acagatgggg
     3361 attaccttcc tgttctcggt gatgtctggc gtgatgtctc gctgcgaccg gatggcggag
     3421 aacgccgata ctcgcggcat tgaacgcgtg aactatctgg cactgctggc actcggcact
     3481 ttctattttc tctgcgcttt cctgcctatc tacttcggcg cggaacatgc aaaaaccatc
     3541 attgatgtcc tgccgcaacg attaatcgac ggcctcggcg tcgcgggcgg catcatgcca
     3601 gcaatcggtt ttgccgtgct gctgaaaatc atgatgaaaa acgtctacat cccctacttc
     3661 atcctgggct ttgttgccgc agcctggctc aagttaccgg tgctggctat tgctgccgct
     3721 gcgctggcga tggcgctgat cgacctgctg cgtaaatctc ctgaaccgac tcaaccttcg
     3781 gcacagaaag aggaatttga agatggcatc taatcaaacc accctgccga acgtcagtga
     3841 aaacgaagaa acattgctga ctggcgtcaa tgaaaacgtg tatgaagatc agagcattgg
     3901 cgcggagctg acgaaaaaag atatcaaccg cgtcgcctgg cgttccatgt tgttacaagc
     3961 gtcttttaac tacgaacgta tgcaggcttc tggctggttg tacggtctgc tgcctgctct
     4021 gaaaaagatc cacactaata aacgcgacct ggcgcgcgcc atgaaggggc atatgggttt
     4081 cttcaatacc catccgtttc tggtgacgtt tgttatcggc attatccttg cgatggagcg
     4141 ttctaagcag gacgttaaca gtattcagag caccaaaatt gccgtcggtg cgccgcttgg
     4201 cgggattggc gatgcaatgt tctggctgac gctactgccg atttgtggcg ggataggcgc
     4261 aagcctcgcg ctacaaggct ctattcttgg cgcagtcgtc tttattgtgc tgttcaacgt
     4321 ggtacacctg gggctgcgtt ttggtctggc gcattatgct taccgcatgg gcgtggcggc
     4381 gattccactc attaaagcga ataccaaaaa agtcggccat gctgcgtcaa tcgttggaat
     4441 gacggtaatc ggcgcactgg tggcaaccta tgttcgttta agcaccacgc tggaaatcac
     4501 agcgggcgac gcagtggtta agttacagac tgatgttatt gacaaactga tgcctgcctt
     4561 cttaccgctg gtctacaccc tgaccatgtt ctggctggta cgccgcggct ggagtccgct
     4621 gcgcctgatt gcagtgaccg tggttctcgg catcgtcggt aaattctgcc atttccttta
     4681 aatacaatga ggtattagat gttaagtatt attttgacag ggcatggcgg gtttgccagt
     4741 ggcatggaaa aagcaatgaa gcaaatcctc ggcgatcaat cgcagtttat tgccatcgat
     4801 tttcccgaaa cctcaagcac cgcgctgctt acatcgcagc ttgaagaggc catcgcccaa
     4861 ctggattgtg aagacggcat tgtttttctg acggatttgc tgggcggcac gccgtttcgc
     4921 gtagcttcaa cgctggcgat gcaaaaaccg ggctgtgaag tgattaccgg caccaatttg
     4981 caactgctac tggagatggt gctggagcgc gaagggttaa gcggtgaaga gttccgtgtg
     5041 caggcgctgg agtgtggaca ccgtgggctg acctgtctgg tggacgagtt aggccgctgc
     5101 catgaggaat gtccggtcga ggaaggaata tgacacacgt tctgcgcgcc agaaggctgc
     5161 tgactgaaga gggatggctc gatgaccatc agttgtgtat tactgacggt gtcatcgcag
     5221 caatcgaacc gattccagtg ggcgtgactg aacgcgatgc ggaactgctc tgccctgctt
     5281 acatcgacac ccatgtacac ggtggtgcgg gcgttgatgt aatggatgac gcgccggatg
     5341 tgttcgacaa gctggcaatg cacaaggcac gcgaaggtgt cggcagttgg ttaccgacca
     5401 ccgtaaccgc gccgcttaat accattcatg cggcgctgaa acgtattgct caacgttgcc
     5461 aacgcggcgg acctggtgcg caagtgctgg ggagttatct cgaaggaccg tacttcacgc
     5521 cgcagaataa aggcgcgcat ccgccggagt tgtttcgcga gcttgaaatt gccgagctgg
     5581 atcagttgat tgccgtttct cagcacacct tacgtgtggt agcgctggca ccggaaaaag
     5641 aaggggcatt gcaggccatc cgccatctta aacagcaaaa cgtaagggtg atgctggggc
     5701 atagcgcggc ggcctggcaa caaacccgcg ccgcatttga tgctggtgcc gacggcctgg
     5761 tgcattgcta taacgggatg acaggtttac atcaccgcga accgggaatg gttggcgcgg
     5821 gattaacgga caagcgcgcc tggctggaac tgatagccga tggtcatcat gtgcatccgg
     5881 cggcaatgtc gctgtgttgt tgctgtgcga aagagagaat cgtactgatc accgacgcga
     5941 tgcaggccgc tgggatgccg gatggtcgct atacgttatg tggtgaagaa gtgcagatgc
     6001 acggtggcgt tgtccgtacc gcgtccggtg ggctggcggg cagtacgcta tctgtcgatg
     6061 cagcagtgcg caacatggtc gagttaacgg gcgtaacgcc tgcggaagcc atccatatgg
     6121 cgtcgctgca tccggcgcga atgctgggtg ttgatggtgt tctgggatcg cttaaaccgg
     6181 gcaaacgcgc cagcgtcgtt gcgctggata gcgggctgca tgtgcaacaa atctggattc
     6241 agggtcaatt agcttcgttt tgatggtttg ctccattatt gggccttcac ttcccccgta
     6301 aggcctttct ttttctttcg ttttgatctg tgcagcggtg ccggatgcga cgctaacgcg
     6361 tctgatccga cctacggtac gccgcatccg gcaatcggtg aacatagcga cgtcattacg
     6421 caattcttta atcttccctt gtcttttctt tcaacgatca cagatttcgt tttatttccc
     6481 ttccctccat tgaactttcg gtttcttttc tatagatttt aatcaacgaa agacatcacc
     6541 aagtgaaatg aaacgaaagg caagtgaaag cgacaacgcc cgacgtcaag ttcatcagac
     6601 taaggattga gttatgccag aaaattacac ccctgctgcc gccgcaaccg atacatggac
     6661 tgaagaagag atccgccatc agcctcgcgc atggatccgt tcactcacca acatcgacgc
     6721 gctacgttcc gcgctcaata acttccttga accgttactg cgcaaagaga atctgcggat
     6781 catcctgacc ggagccggaa cgtcggcatt tatcggtgac atcatcgcgc cgtggctcgc
     6841 cagccatacc ggtaaaaact tcagcgccgt accgaccacc gatctggtca ccaatccgat
     6901 ggactacctg aatccagctc atccgctgct gttgatctcc ttcggtcgat ccggcaacag
     6961 cccggaaagc gtcgccgccg tggaactggc aaatcaattt gtacccgagt gctatcacct
     7021 gccgatcacc tgcaacgaag cgggcgctct ttaccaaaac gcgatcaaca gcgataacgc
     7081 gtttgccctg ctgatgcccg cagaaacgca cgatcgcggc tttgcgatga ccagcagcat
     7141 taccaccatg atggccagct gcctcgcggt tttcgcacct gagacgatca acagccaaac
     7201 cttccgcgat gtggcggatc gttgccaggc gatcctgacc tcactgggtg atttcagcga
     7261 aggtgtgttt ggttatacac cgtggaaacg gatcgtttat ctcggcagcg gtggcttaca
     7321 gggcgcagca cgcgagtcgg cgctgaaagt gctggaactg actgcgggta aactggcggc
     7381 cttctatgat tccccaaccg gattccgtca tggcccgaaa tcgctggtcg ataacgaaac
     7441 gctggtggtg gtatttgtct caagccaccc ttacacccgt cagtatgatc ttgatctgct
     7501 ggctgaactt cgccgtgaca accaggcaat gcgcgtaatc gccatcgccg cggaaagcac
     7561 cgacgttatc gccgctggcc cgcatatcat cctgccgcca tcccgtcact ttatcgacgt
     7621 tgagcaggca ttttgcttcc tgatgtacgc ccagacgttt gcactgatgc agtcgctgca
     7681 catgggcaat acgccggata ccccatcagc cagtggcacc gttaaccgcg tggtgcaagg
     7741 cgtaatcatt catccgtggc aggcataaga ggatcgcatt atgagcatta tctccaccaa
     7801 atatctgtta caggacgccc aggccaatga ctacgcggtg cctgccttta acattcataa
     7861 cgccgagacg atccaggcga tcctcgaagt gtgcagtgaa atgcgatcgc cggtgatcct
     7921 cgccggaacg ccggggacct ttaaacatat cgcgctggaa gagatctacg ccctgtgtag
     7981 cgcctattcc acaacctaca acatgccact ggcgctgcat ctcgaccacc acgaatcgct
     8041 ggatgatatt cgccgtaaag tccacgcagg cgtgcgcagt gcgatgatcg acggcagcca
     8101 cttcccgttt gccgagaacg taaagctggt gaaatcggtt gtcgacttct gccactccca
     8161 ggattgcagc gtggaagcag aactgggccg cctgggcggt gttgaagatg acatgagcgt
     8221 tgacgccgaa agtgcattcc tgaccgatcc acaagaagct aaatgctttg tcgaactgac
     8281 tggcgtcgac agcctggcgg tagcgattgg tacggcgcac ggcttataca gcaaaacgcc
     8341 gaagattgat ttccagcggc tggcggaaat tcgtgaagtg gtggatgttc ctctggtgct
     8401 gcatggtgcc agcgatgttc cggatgaatt tgtccgtcgc actattgaac ttggcgtcac
     8461 aaaagtgaac gttgccacag aattaaaaat agccttcgct ggcgcggtta aagcctggtt
     8521 tgcggaaaat ccgcagggta atgatcctcg ttattatatg cgcgtcggaa tggatgcgat
     8581 gaaagaagtt gtcagaaata aaattaatgt ctgtggttca gcgaatcgaa tttcagcata
     8641 atcattagtt ttttatgatt tatcccaatg tacttcccga ttaatatcgg ggagt
//
LOCUS       DAZPLI010000157         8673 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZPLI010000157 DAZPLI010000000
VERSION     DAZPLI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8673
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.158"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(297..560)
                     /gene="rpsT"
                     /locus_tag="ACHUU3_003519"
     CDS             complement(297..560)
                     /gene="rpsT"
                     /locus_tag="ACHUU3_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR2083503.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            663..881
                     /locus_tag="ACHUU3_003520"
     CDS             663..881
                     /locus_tag="ACHUU3_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR2083504.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            889..1830
                     /gene="ribF"
                     /locus_tag="ACHUU3_003521"
     CDS             889..1830
                     /gene="ribF"
                     /locus_tag="ACHUU3_003521"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR2083505.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1873..4689
                     /gene="ileS"
                     /locus_tag="ACHUU3_003522"
     CDS             1873..4689
                     /gene="ileS"
                     /locus_tag="ACHUU3_003522"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR2083506.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4689..5183
                     /gene="lspA"
                     /locus_tag="ACHUU3_003523"
     CDS             4689..5183
                     /gene="lspA"
                     /locus_tag="ACHUU3_003523"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR2083507.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            5308..5757
                     /gene="fkpB"
                     /locus_tag="ACHUU3_003524"
     CDS             5308..5757
                     /gene="fkpB"
                     /locus_tag="ACHUU3_003524"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR2083508.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5759..6709
                     /gene="ispH"
                     /locus_tag="ACHUU3_003525"
                     /gene_synonym="lytB"
     CDS             5759..6709
                     /gene="ispH"
                     /locus_tag="ACHUU3_003525"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR2083509.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6775..7689
                     /gene="rihC"
                     /locus_tag="ACHUU3_003526"
     CDS             6775..7689
                     /gene="rihC"
                     /locus_tag="ACHUU3_003526"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR2083510.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7856..>8673
                     /gene="dapB"
                     /locus_tag="ACHUU3_003527"
     CDS             7856..>8673
                     /gene="dapB"
                     /locus_tag="ACHUU3_003527"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR2083511.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNN"
BASE COUNT         1979 a         2164 c         2464 g         2066 t
ORIGIN      
        1 aattcaaaaa tagagtggtc ttaagtttaa tacctcaatg tgtatcacaa tatccacatt
       61 ctttgtaggg gagtctggag attgagtaga tattcttgtt cagaatgtat cagccgatgg
      121 ttctacgatt cttaagccac gaagagttca gaaagtgtaa cggcatgtct gcaaaagcca
      181 gcatcttttg actatctggc aaccggtagt gtgttctctc acgcatcata aaagcagcag
      241 gcataaaaaa aacccgcttg cgcgggcttt ttcacaaagc ttcagcaaat tggcgattaa
      301 gccagtttgt tgatctgtgc agtcaggtta gccttatgac gtgcagcttt gtttttgtgg
      361 atcagacctt tagcagcctg acggtccacg atcggttgca tttcgttaaa tgctttctgt
      421 gcagcagctt tgtcgccagc ttcgatagct gcgtatactt tcttgatgaa agtacgcatc
      481 atagagcgac ggcttgcgtt gtgcttacga gccttttcag actgaatggc gcgcttctta
      541 gctgatttga tattagccaa ggtccaactc ccaaatgtgt tctatatgga caattcaaag
      601 gccgaggaat atgccctttt agccttcttt tgtcaatgga tttgtgcaaa taagcgccgt
      661 taatgtgccg gcactcgtta cgtagtgatg gcgcaggatt ctaccagctt gcggggtgtg
      721 aatacagctt ttccgcgata aaaattgcag caggcggtca ctttcttccc gtgatttgcg
      781 ccatggcaat gaaaagccac ttctttctga tttcggtact caatcgccgg ttaaccttga
      841 ccgctgtaca aggtatactc ggacgatttt cactgttttg agccagacat gaagctgata
      901 cgcggcatac ataatctcag ccaggccccg caagaagggt gtgtgctgac tattggtaat
      961 ttcgacggcg tgcatcgcgg tcatcgcgcg ctgttacagg gcttgcagga agaagggcgc
     1021 aagcgcaact taccggtgat ggtgatgctt tttgaacctc aaccactgga actgtttgct
     1081 accgataaag ccccggcaag actgacccgg ctgcgggaaa aactgcgtta ccttgcagag
     1141 tgtggcgttg attacgtgct gtgcgtgcgt ttcgacaggc gtttcgcggc gttaaccgcg
     1201 caaaatttca tcagcgatct tctggtgaag catttgcgcg taaaatttct tgccgtaggg
     1261 gatgatttcc gctttggcgc tggtcgtgaa ggcgatttct tgttattaca gaaagctggc
     1321 atggaatacg gcttcgatat cactagtacg caaacttttt gcgaaggtgg cgtgcgtatt
     1381 agcagcaccg ctgtgcgtca ggcgctggcg gatgacaatc tggctctggc agaaagttta
     1441 ctggggcacc cgtttgccat ctccgggcgt gtagtccacg gtgatgaatt agggcgcact
     1501 ataggtttcc cgacggcgaa tgtaccgcta cgccgtcagg tttccccggt gaaaggggtt
     1561 tatgcggtag aagtgctggg cctcggtgaa aagccgttac ccggcgtggc aaacatcgga
     1621 acacgcccaa cggttgccgg tattcgccag cagctggaag tgcatttgtt agatgttgca
     1681 atggaccttt acggtcgcca tatacaagta gtgctgcgta aaaaaatacg caatgagcag
     1741 cgatttgcgt cgctggacga actgaaagcg cagattgcgc gtgatgaatt aaccgcccgc
     1801 gaattttttg ggctaacaaa accggcttaa gcctgttatg taatcaaacc gaaacacgga
     1861 accgagaatc tgatgagtga ctataaatca accctgaatt tgccggaaac agggttcccg
     1921 atgcgtggcg atctcgccaa gcgcgaaccg ggaatgctgg cgcgttggac tgatgatgat
     1981 ctgtacggca tcatccgtgc ggctaaaaaa ggcaaaaaaa ccttcattct gcatgatggc
     2041 cctccttatg cgaatggcag cattcatatt ggtcactcgg ttaacaagat tctgaaagac
     2101 attatcgtga agtccaaagg gctttccggt tatgactcgc cgtatgtgcc tggctgggac
     2161 tgccacggtc tgccgatcga gctgaaagta gagcaagaat acggtaagcc gggtgagaaa
     2221 ttcaccgccg ccgagttccg cgccaagtgc cgcgaatacg cggcgaccca ggttgacggt
     2281 caacgcaaag actttatccg tctgggcgtg ctgggcgact ggtcgcaccc gtacctgacc
     2341 atggacttca aaactgaagc caacatcatc cgcgcgctgg gcaaaatcat cggcaacggt
     2401 cacctgcaca aaggcgcgaa gccagttcac tggtgcgttg actgccgttc tgcgctggcg
     2461 gaagcggaag ttgagtatta cgacaaaact tctccgtcca tcgacgttgc ttttcaggcg
     2521 gtcgatcagg atgcactgaa agcaaaattt gccgtaagca acgttaacgg cccaatctcg
     2581 ctggtgatct ggaccactac gccgtggact ctgcctgcga accgcgcaat ctctattgca
     2641 cctgatttcg actatgcgct ggtgcagatc gacggtcagg ccgtgattct ggcgaaagat
     2701 ctggttgaaa gcgtaatgca gcgtatcggc gtgaccgatt acaccattct cggcacggta
     2761 aaaggtgcgg agcttgagtt gctgcgcttt acccatccgt ttatgggctt cgacgttccg
     2821 gcaatcctcg gcgatcacgt taccctagat gccggtaccg gtgccgttca caccgcgcct
     2881 ggccacggcc cggacgacta tgtgatcggt cagaaatacg gcctggaaac cgctaacccg
     2941 gttggcccgg acggcactta tctgccgggc acttatccga cgctggatgg cgtgaacgtc
     3001 ttcaaagcga acgacatcgt cgttgcgctg ctgcaggaaa aaggcgcgct gctgcacgtt
     3061 gagaaaatgc agcacagcta tccgtgctgc tggcgtcaca aaacgccgat catcttccgc
     3121 gcgacgccgc agtggttcgt cagcatggat cagaaaggtc tgcgtgcgca gtcactgaaa
     3181 gagatcaaag gcgtgcagtg gatcccggac tggggccagg cgcgtatcga gtcgatggtt
     3241 gctaaccgtc ctgactggtg tatctcccgt cagcgcacct ggggcgtacc gatgtcactg
     3301 ttcgtgcaca aagacacgga agagctgcat ccgcgtactc tcgaactaat ggaagaagtg
     3361 gcaaaacgcg ttgaagttga cggcatccag gcgtggtggg atcttgatgc gaaagagatc
     3421 ctcggcgatg aagctgatca gtacgtgaaa gtgccggaca cattggatgt atggtttgac
     3481 tccggatcta cccactcttc tgttgttgac gtgcgtccgg aatttgccgg tcacgcagcg
     3541 gacatgtatc tggaaggttc tgaccagcac cgtggttggt tcatgtcttc cctaatgatc
     3601 tccaccgcga tgaaaggcaa agcgccgtat cgtcaggtac tgacccacgg ctttaccgtg
     3661 gatggtcagg gtcgcaagat gtctaaatcc atcggcaata ccgtttcgcc gcaggatgtg
     3721 atgaacaaac tgggcgcgga tattctgcgt ctgtgggtgg catcaaccga ctacaccggt
     3781 gaaatggccg tttctgacga gatcctgaaa cgtgctgccg atagctatcg tcgtatccgt
     3841 aacaccgcgc gcttcctgct ggcaaacctg aacggttttg atccagcaaa agatatggtg
     3901 aaaccggaag agatggtggt actggatcgc tgggccgtag gttgtgcgaa agcggcacag
     3961 gaagacatcc tcaaggcgta cgaagcatac gatttccacg aagtggtaca gcgtctgatg
     4021 cgcttctgct ccgttgagat gggttccttc tacctcgaca tcatcaaaga ccgtcagtac
     4081 accgccaaag cggacagtgt ggcgcgtcgt agctgccaga ctgcgctgta tcacatcgca
     4141 gaagcgctgg tgcgctggat ggcaccaatc ctctccttca ccgctgatga agtgtggggc
     4201 tacctgccgg gcgaacgtga aaaatacgtc ttcaccggtg agtggtacga aggcctgttt
     4261 ggcctggcag acagtgaagc gatgaacgat gcgttctggg acgagctgtt gaaagtgcgt
     4321 ggcgaagtga acaaagtcat tgagcaagcg cgtgccgaca agaaagtggg tggctcgctg
     4381 gaagcggcag taaccttgta tgcagaaccg gaactgtcgg cgaaactgac cgcgctgggc
     4441 gatgaattac gatttgtcct gttgacctcc ggcgctaccg ttgcagacta taacgacgca
     4501 cctgctgatg ctcagcagag cgaagtactc aaagggctga aagtcgcgtt gagtaaagcc
     4561 gaaggtgaga agtgcccacg ctgctggcac tacacccagg atgtcggcaa ggtggcggaa
     4621 cacgcagaaa tctgcggccg ctgtgtcagc aacgtcgccg gtgacggtga aaaacgtaag
     4681 tttgcctgat gagtcaatcg atctgttcaa cagggctacg ctggctgtgg ctggtggtag
     4741 tcgtgctgat tatcgatctg ggcagcaaat acctgatcct ccagaacttt gctctggggg
     4801 atacggtccc gctgttcccg tcgcttaatc tgcattatgc gcgtaactat ggcgcggcgt
     4861 ttagtttcct tgccgatagc ggcggctggc agcgttggtt ctttgccggt attgcgattg
     4921 gtattagcgt gatcctggca gtgatgatgt atcgctcgaa ggccacgcag aagctaaaca
     4981 atatcgctta cgcgctgatt attggcggcg cgctgggcaa cctgttcgac cgcctgtggc
     5041 acggcttcgt tgtcgatatg atcgacttct acgtcggcga ctggcacttc gccaccttca
     5101 accttgccga tactgccatc tgtgtcggtg cggcactgat tgtgctggaa ggttttttgc
     5161 cttctagagc gaaaaaacaa taataaaccc tgccggatgc gatgctgacg catcttatcc
     5221 ggcctacaga ttgctgcgaa atcgtaggcc ggataaggcg tttacgccgc atccggcaaa
     5281 aatcctcaaa tataagagca aacctgcatg tctgaatctg tacagagcaa tagcgccgtc
     5341 ctggtgcact tcacgctaaa actcgacgat ggcaccaccg ccgagtctac ccgcaacaac
     5401 ggtaaaccgg cgctgttccg cctgggtgat gcttctcttt ctgaagggct ggagcaacac
     5461 ctgttggggc tgaaagtggg cgataaaacc accttctcgt tggagccaga tgcggcgttt
     5521 ggcgtgccgt caccggacct gattcagtac ttctcccgcc gtgaatttat ggatgcaggc
     5581 gagccagaaa ttggcgcaat catgcttttt accgcaatgg atggcagtga gatgcctggc
     5641 gtgatccgcg aaattaacgg cgactccatt accgttgatt tcaaccatcc gctggccggg
     5701 cagaccgttc attttgatat tgaagtgctg gaaatcgatc cggcattgga ggcgtaacat
     5761 gcagatcctg ttggccaacc cgcgtggttt ttgtgccggg gtagaccgcg ctatcagcat
     5821 tgttgaaaac gcgctggcca tttacggcgc accgatatat gtccgtcacg aagtggtaca
     5881 taaccgctat gtggtcgata gcttgcgtga gcgtggggct atctttattg agcagattag
     5941 cgaagtaccg gacggcgcga tcctgatttt ctccgcacac ggtgtttctc aggcggtacg
     6001 taacgaagca aaaagccgcg atttgacggt gtttgatgcc acctgtccgc tggtgaccaa
     6061 agtgcatatg gaagtcgccc gcgccagccg tcgtggcgaa gagtcgattc tcatcggcca
     6121 cgccgggcat ccggaagtgg aaggtacaat gggccagtac agtaacccgg aagggggaat
     6181 gtatctggtc gaatcgccgg acgatgtgtg gaaactgacg gtcaaaaacg aagagaagct
     6241 ctcctttatg acccagacca cgctgtcggt ggatgacacg tctgatgtga tcgacgcgct
     6301 gcgtaaacgc ttcccgaaaa ttgtcggtcc gcgcaaagat gacatctgct acgccacgac
     6361 taaccgtcag gaagcggtac gcgccctggc agaacaggcg gaagttgtgt tggtggtcgg
     6421 ttcgaaaaac tcctccaact ccaaccgtct ggcggagctg gcccagcgta tgggcaaacg
     6481 cgcgtttttg attgacgatg cgaaagatat ccaggaagag tgggtgaaag aggttaaatg
     6541 cgtcggcgtg actgcgggcg catcggctcc ggatattctg gtgcaaaatg tggtggcacg
     6601 tttgcagcag ctgggcggtg gtgaagccat tccgctggaa ggccgtgaag aaaacattgt
     6661 tttcgaagtg ccgaaagagc tgcgtgtcga tattcgtgaa gtcgattaag tcattagcag
     6721 cctaagttat gcgaaaatgc cggtcttgtt accggcattt tttatggaga aaacatgcgt
     6781 ttacctatct tcctcgatac tgaccccggc attgacgatg ccgtcgccat tgccgccgcg
     6841 atttttgccc ccgaactcga cctgcaactg atgaccaccg tcgcgggtaa tgtctcggtt
     6901 gagaaaacca cccgcaacgc cctgcaactg ctgcatttct ggaatgcgga gattccgctc
     6961 gcccagggag ccgctgtgcc gctggtacgc gcaccgcgtg atgcagcatc tgtgcacggc
     7021 gaatcgggaa tggctggcta cgactttgtt gagcacaacc gaaagccaat cgggatacca
     7081 gcgtttctgg cgattcgtga tgccctgatg cgtgcaccag agcctgttac cctggtggcc
     7141 attggcccgt taaccaatat tgcgctgtta ctttcacaat gcccggaatg taaaccgtat
     7201 attcgtcgtc tggtgatcat gggcggttct tctggtcgcg gcaactgtac accaaatgcc
     7261 gagtttaata tcgctgccga tccagaagcg gctgcctgtg tcttccgtag cggaatcgaa
     7321 atcgtcatgt gcggtctgga tgtcaccaat caggcaatat taacccctga ctatctcgct
     7381 actttgccgg agttgaatcg caccggaaaa atgcttcatg cactgtttag ccactaccgt
     7441 agcggcagta tgcaaagcgg tctgcgaatg cacgatctct gcgccatcgc ctggctggtg
     7501 cgcccggact tattcaccct caagccgtgt tttgtcgcgg tagaaactca aggcgaattt
     7561 acctcaggca cgacggtggt tgatatcgac ggttgcctgg gcaaaccagc caatgtaaag
     7621 gtggcattgg atctggatgt gaaaggcttc cagcagtggg tggctgaggt gctggctctg
     7681 gcgtcgtaac ctgtcacatg ttatcggcat gcagtcattc atcgactcat gcctttcact
     7741 gatatccctc cctgtttatc attaatttct aattatcagc gtttttggct ggcggcgtag
     7801 cgatgcgctg gttactctga aaacggtcta tgcaaattaa caaaagagaa tagctatgca
     7861 tgatgcaaac atccgcgttg ccatcgcggg agccgggggg cgtatgggcc gccagttgat
     7921 tcaggcggcg ctggcattag aaggcgtgca gctgggcgcg gcgctggagc gcgaaggatc
     7981 ttctttactg ggcagcgacg ccggtgagct ggctggtgca gggaaaacag gcgttaccgt
     8041 gcaaagcagc ctcgatgcgg taaaagatga ttttgatgtg tttatcgatt ttacccgtcc
     8101 ggaaggtacg ctgaaccatc tcgctttttg tcgccagcat ggcaaaggga tggtgatcgg
     8161 cactacgggg tttgacgaag ccggtaaaca ggcgattcgt gacgccgccg ccgatattgc
     8221 gattgtcttt gccgccaatt ttagcgttgg cgttaacgtc atgcttaagc tgctggagaa
     8281 agcagccaaa gtgatgggtg actacaccga tatcgaaatt attgaagcac atcatagaca
     8341 taaagttgat gcgccgtcag gcaccgcact ggcaatggga gaggcgatcg cccacgccct
     8401 tgataaagat ctgaaagatt gcgcggtcta cagtcgtgaa ggccacaccg gtgaacgtgt
     8461 gcctggcacc attggttttg ccaccgtgcg tgcaggtgac atcgttggtg aacataccgc
     8521 gatgtttgcc gatattggcg agcgtctgga gatcacccat aaggcgtcca gccgtatgac
     8581 atttgctaac ggcgcggtaa gatcggcttt gtggttgagt ggtaaggaaa atggtctttt
     8641 tgatatgcga gatgtactta atctcaataa ttt
//
LOCUS       DAZPLI010000158         8668 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZPLI010000158 DAZPLI010000000
VERSION     DAZPLI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8668
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.159"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(301..507)
                     /locus_tag="ACHUU3_003528"
     CDS             complement(301..507)
                     /locus_tag="ACHUU3_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR2083512.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            820..909
                     /gene="kdpF"
                     /locus_tag="ACHUU3_003529"
     CDS             820..909
                     /gene="kdpF"
                     /locus_tag="ACHUU3_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR2083513.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            909..2582
                     /gene="kdpA"
                     /locus_tag="ACHUU3_003530"
     CDS             909..2582
                     /gene="kdpA"
                     /locus_tag="ACHUU3_003530"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR2083514.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLIALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2605..4653
                     /gene="kdpB"
                     /locus_tag="ACHUU3_003531"
     CDS             2605..4653
                     /gene="kdpB"
                     /locus_tag="ACHUU3_003531"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFR2083515.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4662..5234
                     /gene="kdpC"
                     /locus_tag="ACHUU3_003532"
     CDS             4662..5234
                     /gene="kdpC"
                     /locus_tag="ACHUU3_003532"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR2083516.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5227..7911
                     /gene="kdpD"
                     /locus_tag="ACHUU3_003533"
     CDS             5227..7911
                     /gene="kdpD"
                     /locus_tag="ACHUU3_003533"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFR2083517.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7908..8585
                     /gene="kdpE"
                     /locus_tag="ACHUU3_003534"
     CDS             7908..8585
                     /gene="kdpE"
                     /locus_tag="ACHUU3_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFR2083518.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
BASE COUNT         1820 a         2248 c         2491 g         2109 t
ORIGIN      
        1 acaatgctac cgccatactg cgtcatgtcg ccctgacgcg ccgccggttt tccgctcatt
       61 tctcatcctt gttttatcgg gttatttgtc cgctgacagg caagacccta cagatttata
      121 agaaagcctt aagtgagata tccgtcagat agaaacaatt aggaaaatat ttatatatat
      181 ttcatgaata tatgtttcga ttggtataac ggtgtctaaa tattcctctg aacatcttcc
      241 tgatacaaaa aaccgccagc gtttccgctg gcggttttca atgtattcag aactcagcaa
      301 tcagtaaaaa tcaccggttg ccttttcggc ctgatccatc cacaccggtt tatcgctggt
      361 tttcgaccag acgcggtgca ggtagctgta aaagcgggcg cggtctttcc agaaaagcat
      421 gaaaggcagc gccagaacgc ccgctgcaac cgcatatgta cggcgtaaaa agataagcca
      481 tgcaggatat tctctgtaga gttccatact tttctccccc ttttgtatct acccggtgaa
      541 tagcaccgga aaaatgaatt tgtttatctg atgaaaatag taccgccttt tgtgtaattt
      601 tactactcat ccgaccactt atttttgctt attgatggtt tatttacatt cactctgtaa
      661 ttaagttaca caaaagttaa attaatacta aacattagtt aaatcatggc ttttgccatt
      721 tttatacttt ttttacaccc cgcccgcaga tttttgcgaa atctttgcag ccagaattct
      781 actcttccgg tatcactttt aggccactgg aggtgcactg tgagtgcagg cgtgatagcc
      841 ggcgtgttgc tggtgttttt attactgggt tatttggttt atgccctgat caatgcggag
      901 gcgttctgat ggctgcgcaa gggttcttac tgattgccac gtttttactg gtattaatgg
      961 tgctggcgcg tcctttaggc agcgggctgg cgcggctgat taatgacatt cctcttccag
     1021 gtacagcggg cgttgaacgt atactctttc gtttacctgg cgtctctgac catgagatga
     1081 actggaagca atatctttgt gccattctcg gcctgaacat gctggggctg gcggtgctgt
     1141 tttttatgtt gctcggtcag cactatctgc cgcttaatcc acagcagttg ccggggctgt
     1201 cgtgggatct ggcgctgaat accgccgtca gctttgtcac gaataccaac tggcaatctt
     1261 atagcggtga aaccacgttg agctatttca gccagatggc aggcttaacg gtacaaaact
     1321 ttctttctgc cgccagcggg attgcggtga tttttgcctt catccgtgcg tttactcgcc
     1381 agagcatgag cacactcggg aatgcctggg tcgatctgct acgcatcacg ttatgggtgc
     1441 tagtccctgt ggcgttgttg atcgcactgt tttttattca acaaggtgcg ctgcaaaact
     1501 ttctgcccta tcaggcagtg aataccgttg aaggagcgca acagctgtta cccatgggac
     1561 cagtagcttc tcaggaagcg atcaagatgc tcggtactaa cggcggtggc ttctttaatg
     1621 ccaactcgtc gcatccgttt gaaaacccaa ccgcactgac caacttcgtg cagatgctgg
     1681 cgatcttctt gatcccaaca gcgctgtgct ttgcctttgg tgaagtgacg ggcgatcgcc
     1741 gccaggggcg catgttgctg tgggcgatgt cagtgatttt tgtcatctgc gtaggcgtgg
     1801 ttatgtgggc agaagttcag ggtaatcctc atctgctggc gctcggcgca gacagcagca
     1861 tcaatatgga aggtaaagag agccgtttcg gcgtgctggt cagtagcctg tttgcggtcg
     1921 tgactacggc ggcgtcctgt ggcgcggtga ttgcgatgca tgattcgttt accgctctcg
     1981 gcggcatggt gccgatgtgg ctaatgcaaa ttggtgaagt ggtgttcggc ggtgtcggtt
     2041 ctggtcttta cggcatgatg ctgtttgtcc tgctggcggt gtttattgcc gggctgatga
     2101 ttggtcgtac accggaatat ctgggtaaaa aaatcgacgt acgcgagatg aaactgattg
     2161 cactggcgat tctggttact ccaacactgg tactgatggg cgcggcgttg gcgatgatga
     2221 ccgacgccgg acgtagcgcc atgcttaacc ctggcccgca tggttttagc gaagtgctgt
     2281 acgccgtgtc gtccgccgct aacaacaacg gcagcgcctt tgccggatta agcgccaact
     2341 ctccgttctg gaactgttta ctggcgttct gcatgtttgt cggtcgcttc ggggtgatta
     2401 tcccggtgat ggcaattgcc ggttcgctgg tgagtaaaaa gagccaaccc gccagctccg
     2461 gcacgctgcc aacgcacggc ccgctgtttg ttggcctgtt aatcggcacc gtgttgctgg
     2521 ttggcgcact gacctttatc cctgccctgg cgcttggtcc ggtggcggaa tatctctcct
     2581 gatgatattg agcgagcact gaatatgagt cgtaaacaac tggcgctatt cgaaccaaca
     2641 cttgtcgttc aggcgctgaa agaagcggtg aaaaaattaa acccgcaggc gcaatggcgc
     2701 aatccggtga tgtttatcgt ctggatcggc agtctgctga ccacctgtat tagcatcgcg
     2761 atggcaagcg gtgcgatgcc tggcaatgcg ctgtttagcg cggcaattag cggctggctg
     2821 tgggtcaccg tactgttcgc taatttcgcc gaggcgctgg cagaaggccg cagtaaagcg
     2881 caggccaaca gtcttaaagg ggtgaaaaaa actgcctttg cccgcaagct gcgtgagccg
     2941 aaatatggcg ctgcggcgga caaagttcct gccgatcaac tgcgtaaagg tgacatcgtg
     3001 ctggtagaag ctggcgatat tatcccctgc gatggtgaag tcattgaagg cggcgcatcg
     3061 gtcgatgaaa gcgccatcac cggggaatcg gcaccggtga tccgtgaatc cggcggcgat
     3121 tttgcctccg tcaccggcgg cacgcgtatt ctttctgact ggctggtgat tgagtgtagc
     3181 gttaaccccg gtgagacatt tctggatcgg atgatcgcga tggtggaagg cgcacagcga
     3241 cgcaaaacac cgaacgagat tgccctgact atcctgctga ttgctctgac catcgtcttt
     3301 ttactggcaa ccgccacgct gtggccgttt tccgcgtggg gcggtaatgc cgtgagtgta
     3361 acggtactgg tggcgctgct ggtctgtctg atcccaacca ctattggcgg cctgttgtca
     3421 gcgatcggcg tcgccgggat gagtcggatg ctgggcgcga atgtgattgc caccagcgga
     3481 cgtgcagttg aagcggcagg tgacgttgac gttctgctac tggataaaac cggcaccatc
     3541 acactcggta accgtcaggc gtcggagttt atccccgcgc agggcgtgga tgaaaaaacg
     3601 ctggctgacg ccgcacaact ggcttcgctg gctgatgaaa cgccggaagg tcgcagtatt
     3661 gtgatcctcg ccaagcaacg ttttaacctg cgcgagcgcg atgtgcagtc gctccatgcc
     3721 acctttgtac cgtttactgc gcaaagccgg atgagcggga tcaacatcga caaccgcatg
     3781 atccgtaaag gttctgtcga tgccattcgc cgccatattg aggctaacgg cggtcacttc
     3841 cctgccgatg ttgatcaaaa agtcgatcag gttgcgcgtc agggagccac gccgctggtg
     3901 gtggtggaag gttctcgtgt gctgggcgtt attgcgctga aagatatcgt caaaggcggt
     3961 attaaagagc gcttcgccca gctgcgcaaa atgggcatta aaacggtgat gattaccggc
     4021 gataaccgtc tgactgccgc cgcgatagct gcggaagcgg gtgtcgatga ttttctcgcc
     4081 gaagcaacac cggaggccaa gctggcattg atccgtcagt atcaggcgga aggtcgtttg
     4141 gtagcgatga ccggcgacgg caccaacgat gctccggcgc tggcgcaggc agatgtcgcg
     4201 gtggcgatga actccggcac ccaggcggcg aaagaggcgg gcaatatggt ggatctcgac
     4261 tctaacccga ccaagttgat cgaggtggtg cacattggca aacagatgct gatgacccgt
     4321 ggctcgctga ccaccttcag cattgccaac gatgtggcga aatacttcgc cattattccg
     4381 gcggcgttcg cggcaacata tccgcagtta aatgcgctga acatcatgcg cctgcattcg
     4441 cccgactccg ccattctcag tgcggtcatt ttcaacgcct tgattatcgt ctttttgatt
     4501 cctctggcgt taaaaggcgt gagttataaa ccgcttaccg cttctgccat gttgcgccgt
     4561 aacttatgga tttacggtct gggtgggctg ctggtgccgt ttattggtat caaagtcatt
     4621 gatttactgc tgaccgtttg cggtctggtg tgaggtttac catgagtgga ttacgtccgg
     4681 cattatcgac atttctcttt ctgttattga ttactggcgg cgtttacccg ctgctgacca
     4741 ccgcactggg gcaatggtgg tttccctggc aggccaacgg ttcgttgatc cgtgaaggtg
     4801 atacggtgcg tggctcggca ttaatcgggc agaattttac cggcaacggc tattttcatg
     4861 gtcgcccgtc agcaacggct gaaatgccct ataatccaca ggcttctggc gggagcaatc
     4921 tggcggtcag taaccctgag ctggataaac taatagccgc acgcgttgct gcattacggg
     4981 ccgctaaccc ggatgccagc gcgagcattc cggttgaact ggtgacggca tcggcaagcg
     5041 ggctggacaa taatatcacc ccgcaagcgg cggcctggca gatcccacgc atcgcgaaag
     5101 cgcgtaatct cagcgttgaa cagctcacgc agctgatcgc aaaatacagc caacaaccgc
     5161 tggtgaaata tatcggccag ccggtcgtca acattgtcga actcaatctg gcgctggata
     5221 agcttgatga ataacgaacc cttacgtccc gaccccgatc gtctgctgga acaaactgcc
     5281 gcgccgcatc gggggaagct gaaagttttc ttcggtgcct gtgcaggcgt cgggaagacc
     5341 tgggcgatgc tggcagaagc ccagcgactg cgggcgcaag ggctggatat tgtggttggc
     5401 gtggtagaaa cccacgggcg aaaagatacc gccgcaatgc tggaagggct ggctgttctg
     5461 ccgccaaaac gcctggcgta ccgtgggcgg cacatctgcg agtttgacct cgatgccgcc
     5521 ctcgcccgcc gtccggcgct gatcttaatg gacgaactgg cgcacagtaa tgcgccaggt
     5581 tctcgtcacc ctaaacgctg gcaggacatc gaagagctgc tggaagcagg cattgatgtt
     5641 ttcactaccg tcaacgttca gcatctggaa agtctgaatg atgtggtcag cggcgtcacc
     5701 ggaattcagg tacgggaaac cgtgcccgat ccttttttcg atgccgccga cgacgtggtt
     5761 ctggtggact tgcccccgga cgatctgcgc cagcggctga aagaaggcaa agtctatatt
     5821 gccgggcagg cggagcgcgc cattgaacat tttttccgca aaggcaatct gatcgccctg
     5881 cgcgaactgg cactgcgccg tactgccgat cgcgttgatg agcaaatgcg cgcctggcgg
     5941 gggcatccta gcgaagagaa agtctggcac acgcgcgacg cgatcctttt atgcatcggc
     6001 cataacaccg gtagtgagaa gctggtccgc gcggcggcgc ggctggcgtc acggctgggt
     6061 agcgtctggc acgcggtgta tgttgaaacc cctgccctgc atcgcttacc ggagaaaaaa
     6121 cgtcgtgcaa ttctcagcgc cttacgtctg gcgcaggaac tgggcgcgga gacggcaaca
     6181 ctttctgatc cagcggaaga gaaagcggta gtgcgttatg cccgtgaaca taatctcggc
     6241 aagattattc tcggtcgccc ggcctcgcgc cgctggtggc gtcgggaaac gtttgctgac
     6301 cgactggcgc gcatcgcccc cgatctcgat caggtgctgg tcgcgcttga tgaaccaccc
     6361 gcccgcacga ttaacaacgc gccggatagc cgctctttta aagacaagtg gcgggtacag
     6421 attcagggat gcgtggttgc cgccgcgtta tgcgccgtta tcaccttaat tgccatgcag
     6481 tggctgatgg cgtttgatgc cgccaacctg gtgatgctgt atctgcttgg cgtggtggtg
     6541 gtggcgctat tttatggacg ctggccttcg gtggttgcca ccgtcattaa tgtggtgagt
     6601 ttcgatctct tttttatcgc cccacgcggc acgctcgccg tctctgatgt gcaatatctg
     6661 ctgaccttcg cggtgatgtt aaccgtcggg ctggtgatcg ggaaccttac tgctggcgtg
     6721 cgttatcagg cgcgggtagc ccgttaccgc gagcaacgca cacggcactt atatgaaatg
     6781 tcgaaagctc tggcggtggg ccgcagtccg caggacatcg ccgccaccag cgaacaattt
     6841 attgcctcca cgtttcatgc ccgcagtcag gtgttgttgc ccgatgacaa cggtaaattg
     6901 cagccgttaa cacatccgca aggaatgacg ccatgggacg atgccatcgc gcagtggagt
     6961 tttgataaag gcctgcctgc gggcgcgggc accgacacgt tacccggtgt accgtaccag
     7021 attttgccgc taaaaagcgg cgagaaaacc tacgggctgg tggtggtgga gccggggaat
     7081 ctgcgccagt tgatgatccc ggaacagcag cgcctgctgg agacgtttac gctgttagtc
     7141 gccaatgccc ttgagcggct gacgctaacc gccagcgaag aacaggcgcg gatggcaagc
     7201 gaacgtgaac agatccgcaa cgccctgctg gcagcgcttt cgcatgattt acgcacgccg
     7261 cttacggtgc tgtttggtca ggcagaaatc ttaacgctcg atctggcaag cgaaggatca
     7321 ccccacgccc gccaggccag cgagattcgt cagcatgtgc tgaacactac ccgactggtg
     7381 aataatctac tggatatggc gcgaattcag tccggcggct ttaatttgaa gaaagagtgg
     7441 ttaacgctgg aagaagtagt cggcagcgcg ctgcaaatgc tggaaccggg tttatcgtcg
     7501 cccatcaatc tttctctgcc agaaccgctg accttaatcc acgttgacgg gccactcttt
     7561 gaacgggtgc tgattaatct gctggagaac gcggtgaaat atgcgggtgc gcaggccgaa
     7621 attggtatcg atgcccactt tgagggcgaa aatctacaac tggatgtctg ggataacggc
     7681 cccggtcttc cgccaggcca ggagcagacg atatttgata agtttgctcg cgggaataaa
     7741 gagtcggcgg tgccgggtgt cgggcttggg ttggcaattt gtcgggcgat tgtggatgta
     7801 cacggcggca ccattaccgc gttcaaccga ccggaaggtg gtgcctgttt tcgtgttaca
     7861 cttccccagc aaaccgctcc tgaacttgaa gactttcatg aggatatgtg acaaacgttc
     7921 tgattgttga agatgaacag gctattcgtc gctttctgcg cacggcgctg gagggcgacg
     7981 ggatgcgcgt ctttgaggcc gaaactctgc aacgcggctt gctggaagcg gcaacccgta
     8041 agccagattt gattattctc gatctcggcc tgcccgatgg tgatgggatt gagtttatcc
     8101 gcgacctgcg ccagtggagc ccggtgccgg tgattgtgct ttccgcacgc agcgaagaga
     8161 gcgacaagat cgccgcgctg gatgccggag cggatgatta tctgagtaag ccgtttggca
     8221 ttggcgaatt gcaggcccgt ctgcgcgtcg ctttacgccg ccactccgcc accgccgcgc
     8281 ccgatccgct ggtgaaattt tccgatgtta ccgtcgattt agccgcccgc gtgattcacc
     8341 ggggtgagga agaggtgcat ctcacaccaa ttgagttccg cctgctggcg gtgctgctca
     8401 acaatgccgg aaaagtactc acccagcgcc agctccttaa ccaggtgtgg gggccaaacg
     8461 cggtcgaaca cagtcactat ttgcgtattt atatgggaca tctgcgacaa aaactggaac
     8521 aggatccggc ccggcctcgc cacttcatta ctgaaaccgg tattggatat cggtttatgc
     8581 tttgaattgt tattcaataa attcgcttaa atagtgatta atttattatt aaacctgcgc
     8641 cgaatattat tatttggcag gtttaatt
//
LOCUS       DAZPLI010000159         8633 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZPLI010000159 DAZPLI010000000
VERSION     DAZPLI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.160"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHUU3_003535"
     CDS             complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHUU3_003535"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR2083519.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHUU3_003536"
     CDS             complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHUU3_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR2083520.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4609..5766
                     /gene="mdoC"
                     /locus_tag="ACHUU3_003537"
     CDS             4609..5766
                     /gene="mdoC"
                     /locus_tag="ACHUU3_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR2083521.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="ACHUU3_003538"
     CDS             complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="ACHUU3_003538"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR2083522.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="ACHUU3_003539"
     CDS             complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="ACHUU3_003539"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR2083523.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7825..8136)
                     /gene="ymdA"
                     /locus_tag="ACHUU3_003540"
     CDS             complement(7825..8136)
                     /gene="ymdA"
                     /locus_tag="ACHUU3_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR2083524.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="ACHUU3_003541"
     CDS             complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="ACHUU3_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR2083525.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2155 a         2247 c         2051 g         2180 t
ORIGIN      
        1 cataacgccg gtatttttat tggctcgttg tttttgccgg atgcgacgtg aacgctttac
       61 ctaacccaca aaagcttgca aaatcaataa attgcatgaa cgatgtaggc ctgataagcg
      121 tagcgcatca ggcaactaca tttttattgc gaagccgcat ccggtttacg caatgccagt
      181 ggattgagct ttatcccttc gtaataactc acccatgaag aatatctctc aggggaattc
      241 cagacgcgga aatgcagacg ggccatcgtc accggatcgc ttagcagcac aaggcgacga
      301 tcgcgattca gcttctctgg cgtctcgttc agcgcctgtt caacgtggcg gtcacgggcg
      361 atttccagca ccttactggc gcggtgacgc gcggtcgcca ttgcggttgc cagagcgtta
      421 aatgacgggt taaacactgc gtgcataaag ccatcgtcaa gggagcgttg gcgattcatc
      481 tcaaggaacc gatcggtatc aaccagcacc tgcggcggtg aatactcttc cgggatcagg
      541 aacagtttcc agcgtttggt gcgcagacca acggtggcac ggctggaaat caccgaaaca
      601 aacggtgaca ggatcaacga gaagacaatc ggtgccagcc agaacaggaa acgcagatcc
      661 agccacgcca tcccaacagc ccacactaac cctaacagca gctgtgagcc gtggcgtttg
      721 aacgcttcac cccaggaagt ggagtcatca tcacgctgcg gtgaattcca caccacttcc
      781 cagccaagga acgcgctgac gacgaagacc gtatggaaca gcatgcgtac cggagccagc
      841 agcacggaaa acagcacttc cagcagcaac gataatgtaa cgcgccagaa gccgccgtat
      901 tctttcgttc ctttgcacca gataagcaaa atgctcaata acttcggcag gaacaacagc
      961 accatggtcg aagcaaaaag tgcaatcgcc agctcaggac gccactgcgg ccacaccggg
     1021 aacaactgcc gtggttgcag gaagtattgc ggttcggtca acgcatgtac tacctgcaat
     1081 gcagtagaga gcgcgaggaa cataaaccac agcggagcgg agagataaga catcacgccc
     1141 gtcaggaaca ccgcacggtg aaccgggtgc atacccttca ccaggaacag acggaagttc
     1201 atcaggttac cgtggcacca tcggcggtca cgttttagct catcaagcaa gttaggcggc
     1261 aattcttcat aagaacccgg gagatcgtaa gcaatccaga ccccccaacc tgcacggcgc
     1321 atcaacgccg cttccacgaa gtcatgtgac aggattgaac cggcaaagga accttcgccc
     1381 ggcagcggag ccagtgcgca gtgctcgata aacggtttca cgcggataat cgcgttatgt
     1441 ccccagtagt gcgactcgcc aagttgccag aagtgcaaac cggctgtaaa tagtggccca
     1501 tacacgcggg tcgcgaactg ctgacagcgc gcatacagcg tatccatacc ggacgctttc
     1561 ggcgacgact gaatgatccc ggcgttcggg ttggcttcca tcaggcgcac cagcccgcac
     1621 aaacaatcac cggtcattac cgagtcagca tccagcacca ccatgtagct gtactggctg
     1681 ccccagcgac ggcagaagtc atcgatatta ccgcttttac gcttcacgcg gcgacggcgg
     1741 cggcgataga aaatctgacc ttcgccaccg acttcagcga taagctccat ccaggctttt
     1801 tgctctgcga cgcagatatc cgggttataa ctgtcactaa gaatgtagac atcaaagtgt
     1861 ttggcattcc cggtggcttt tactgattcc cacgttgcac gcaggccagc aaaaacacgg
     1921 ttcacgtctt cgttacagat aggcatgatc aacgccgtgc gatgctccgg gtttaatggt
     1981 tcatcgccaa ctgttgacgc agatatactg tatttatcgc gaccaataag cagttgcagg
     2041 aagcccatta acgccgtcca gaatccggcg gacacccaac agaacagtac cgcaaagagg
     2101 atcaggatac cggtttgcag catataaggc agaagctgca taaaggaaac ccacaaatcc
     2161 tgaccaacca tatccatagg attaatcagc gcccaaccct gataaggaag aatggtcttc
     2221 atataccagg tcgcgacgac agtttgcgcg agcgtcagga tcaacagaat gtaacggcgg
     2281 atggtaccga cggtacgcca cttttgctcg ctctcctgct cttctttggt caaacgagcc
     2341 agatagcgcg gcgtgacatc gcgtccacgc aggcgatccc agaaacggcc taccgggttg
     2401 gtacgccacg ggtcgggaaa catcgaggag cgttttgctt ctggcatcgc cttcagctga
     2461 tcgcgccctt cgtcgtcttt aattaactgt ccatcagcaa gtgaatctgg ccaggcttgt
     2521 tccagacgcg cctttaccga gccttgcggg gaatcatcct cccgcgccca ggtgcggtgt
     2581 tcggcatcca gcgcctgatg aacggcgcgg atatcagtct tcggcaatgc cgctttctcg
     2641 cttgcggcga tgggcattgc gtcaatgtac tcagttgtct tattcattgg caggtaactg
     2701 gtagctccag gtttcactca acgtctgatc ggcattcacc agcgcagcac gcatttcagt
     2761 ggttttcttg gcatctttca ctttcacacg catcaccaga cgccagcctt tggtaaccgg
     2821 gttatagcgc accgtgcttt caactatctc accattatca ccaatgctgg tttgcgctgt
     2881 gaccggggta tcctctggca gttttttcat ctctgcgccg gtaaaatcga ccacaaaggc
     2941 gatagtaccg tcaggctggc gaatcaggtt cgactgcttc acatcccccg ttgaacgacg
     3001 cgtttgttgc acccatgcgt tatctggcgc atgcagtttg tcttcatcac ggctgaaggt
     3061 gatggtgtat ttaaagttca tctctttacc cggctccggc agctgatccg gcgtccagta
     3121 agcgacgatg ttatcgttgg tttcatcgtt ggttggaatt tccaccagct caacgctgcc
     3181 tttaccccac tcccctttcg gagtcaccca tgcgcttgga cgaagatcgt aacgatcatc
     3241 gagatcttca aagcgggaga aatcacgacc gcgctgcaac agaccgaagc cttgcgggtt
     3301 ttccatcgag aagctgctaa ccgctaaatg tttcgggtta ttcaacggac gccagatcca
     3361 ttcgccatta ccggcatgga tagagagacc gttagagtcg tgcaactccg gacgatagtt
     3421 atttgcaggc gacggttggt tcggcccaaa caggaacata ctggttaacg gtgcaacccc
     3481 cagtttgccg actttatcgc gcagatagat tttcgactgc acatccacaa ccgtgtcacg
     3541 ccctggcata accacgaatt tgtaagcacc tgtcgcgcgc ggcgagtcaa gcaatgcata
     3601 aatggttaaa cgtttatcag tcggttttgg acgctcgatc cagaactctt tgaagcgtgg
     3661 aaattcttcc cccgatggca aggcggtatc aattgccagg ccgcgggcag aaaggccata
     3721 aacctgacct gcaccaatca cgcggaaata gctggccccg agcatgctga cgatttcatc
     3781 gtttttatct ttgctgttga tcgggtaaag cactttaaaa ccggcaaaac caaggtcttt
     3841 taccgtgtct ttgtcatgct gaacatcgcc gaaagtgaaa taatccgggc tgtatttgat
     3901 tcgtttgact gcggtggcag tcacttcatt tattttgacc ggggtatcga agtacatacc
     3961 ctgatggtag aactcgagtt tgaatggggt cttcagattg ttccagtacg ctttgtcatg
     4021 attaaactgg atctgctgat agtccgcgta tttcatatcg cggaaaacgg agggcaagtt
     4081 gcttttgggc gcctcatagc ctttcccggc taaggattga gcttgctttg cgacatcatc
     4141 aatactgaaa gcccagcttg aagatgtata cagggttaac attactgcag cactcaacca
     4201 acgcattttc atcatttgta gtttatgttt cataattagt aagcacttcc ccctttgtgt
     4261 gcttatatcg atccgatcta ttttaatgga taaccagcca ttccgacaac agaatcccgg
     4321 tatcgttcag cacgctggct catggattaa gcacctgatt gcaaccgttt tcactttccg
     4381 tgcttctcct aaagacagaa gcatcgactt cgtgtagggt ttgccagcaa ttttatttat
     4441 caaccgtctt acagacgctt ccattctggt tagtggtagc gatgatgcta tgcataaaaa
     4501 ccttgaagcc tgcgcctggc gcggtcaggt cggtgtcgct atggcaacaa gcctaaaggc
     4561 ataacccgac agggataaga cgaaaaatcc gagattagtt aaccatatat gaatccagta
     4621 cccgcgcaac gtgaatattt cctcgactcc atccgcgcct ggctgatgtt gttagggatc
     4681 ccttttcata tttctttaat ctattcgagc catacatggc atgtgaatag cgccgaaccg
     4741 tcattgtggc tgaccctttt taatgacttc atccactcgt tccgcatgca ggtatttttc
     4801 gttatatccg gctacttttc ctacatgctt tttttacgct atcccttgaa aaaatggtgg
     4861 aaagtacgtg tcgaacgtgt aggtatcccg atgttaacag ccatcccctt actgacattg
     4921 ccgcaattta ttatgctgca atacgtcaaa gggaaagcgg aaagttggcc tgggctgtca
     4981 ttgtatgaca aatataatac gttggcctgg gaattaatat cacacctgtg gtttttactg
     5041 gtgttagtag tcatgacgac gctgtgcgta tggatattta aacgcatcag aaataattta
     5101 gaaaattctg ataaaacgaa taaaaaattc tcgatggtaa aactatcggt gattttttta
     5161 tgcctcggca tcggctatgc ggtaataaga agaacgattt ttattgtgta tccgcccatt
     5221 ctgagtaatg gcatgttcaa ttttattgtc atgcaaacgc tattttattt gccgttcttt
     5281 atcctcggtg cactggcttt cattttccct catcttaaag ccttgtttac cacgccgtct
     5341 cgtggctgta cccttgcagc agcattggca tttgtcgctt atttactcaa ccagcgctat
     5401 ggcagtggcg atgcctggat gtacgaaacc gagtcggtga tcaccatggt cctcggtctg
     5461 tggatggtga atgtggtctt ctcctttggc caccgtttgc ttaacttcca gtcagcgcgg
     5521 gtgacttact ttgttaacgc atcgctattt atctatctgg ttcaccaccc gttaacgctg
     5581 tttttcggcg catacattac accgcacatc acctccaact ggcttggttt tctctgtggc
     5641 ctgatattcg tagtagggat tgcgataatt ctgtatgaaa ttcatttacg catcccgtta
     5701 ctgaagtttt tgttttctgg taaaccggtt gttaagcgtg agaacgataa agcaccagcc
     5761 cgttaagcca catttacaat aaccattcca cgggcaatat cgacgccagt ctgaccataa
     5821 cccgcttcca gaaactggtg gcgggttctt ttttgagaat aatctctttc tttgcatgac
     5881 gatcaaccca gttgatccgt ccccacctgt ccagacggag ctgccaggcc gcatcatact
     5941 ggctctgaat aaagcgttta tcaattaact gtgccagcgt ttcgctctct atcacgaagc
     6001 ccatttcagt attgagcaat gttgaacgcg gatcgaaatt gaaagagccg ataaacaccg
     6061 ttttaccatc gatgctaaaa gttttagcat gcaggctggc tccggaatta ccggttatgc
     6121 cgcgatcgtg taacgtacta ctttgttcac gcgtcggctt gagttcatat aattccacgc
     6181 catagcggag caattttttg cgccagcgcg cgtatccggc atggacgaca gcaacatcgt
     6241 tagcggcaag agaattggtt aggatcgcaa tctttacccc ttttctcacc atccgtaaga
     6301 gttgcgccac acctgcgcgt gtcggtacaa aataggaaga gataatatcg atgcgttcac
     6361 tgggtgagcc catgatatcg gacaggcgct gcggtaacag tgaatgccgt tttgccttgc
     6421 cctccccttt cgccggatca tcacttaata aacgtgtttt cgcccagata agcggcaacg
     6481 ttccatcaac cagatgattt ataaacggac tggattccat ttggcgtaag taacgatgcg
     6541 tcatggcatc gttatgccag gaggcgggta actcgatgcg atccgccatt tcaccttccg
     6601 ggacatccag cacctgctgt aagggtgaaa ccgatttgca ataccagtag cgggcgaaat
     6661 catcggcaac gtcctctacc acgggtccta ttgccataac atctaaatcc gaaaaaagtg
     6721 gctcttcccc tgccccaaaa taggcatcac caatatttcg tcctcccacc agggtaacca
     6781 cgccatcgac agtgaaactt ttattgtgca tacggcgatt gagacgggaa aagtcggtga
     6841 tataaccaag cggacgcagc aagcgaaacg agaaaggatt aaaaagccgg acttcaatgc
     6901 ggggatggct gtcaagcaag cgtaaaatat cgtcaagtcc gggcgtattg ttgtcgtcca
     6961 gcaacaaacg gacgcgaacg ccacgctttg ctgcggctaa cagggcggaa aacagtaacc
     7021 gacccgacat atcgtcctgc cagatgtaat actgaacatc cagcgtatgc tctgccattt
     7081 cggccagacg ataccgggcg gcaaacgcat ccagactttt ctccagaggg aaaagaccac
     7141 actgaccggg atgttgcgaa cacagtggca gcaccgcgct cgccagccgg ggcaaatcat
     7201 tcatctcctt gttgggtaag gagtctttcg tagaggtggg cgttttcttc atcataacag
     7261 acaaagtata cctgttcggg taaagcgtga cgggtaataa attctgaaac ggttttaact
     7321 gcgatttcag ccgctgccgc acgagggtaa ccataaaccc cagtactgat tgcaggaaaa
     7381 gccactgacg tatagctgtt tgccgccacc agtcgcaggc tattcagata ggcatcctgc
     7441 aaaagctggt cttcgttttg ttcaccacca cgccagactg gcccgacggt gtgtactacg
     7501 gctttagcgg gaagatcgcc tgcaagcgta ataacggcat gccccgtagg gcaatcgccc
     7561 tgctgttgcc tgacttttaa acaggcatcc agcagggccg gacccgctgc gcgatgaatg
     7621 gccccatcga cgccgccgcc tcccattaat gacggattag ccgcattcac aatcacatca
     7681 acggccagtt tggtaatatc accctgcaca acatgaatac gcgttttcat aacgcctcct
     7741 tacaccttct tgtttcttaa gtgtacagca ggctttttgg ttaaatcgca tttttctgct
     7801 cagcaacgtg cgttgttcag aaacttagtt atattcgatg ttcattacag ccagcttttt
     7861 ctgtttatca agatactgca cttttactga ggctatttgc gcgtttttaa cattaccgct
     7921 cattaacgct ttgctggaat aggttttgcc cggaacagaa ccattctgtg ggcagttcat
     7981 ttctacgggt gatgactggg tgctgacgtc acatgccggt tccacaattt ggccataaaa
     8041 atggatcaca cccccttgcg cggtgcttcc tgcgatggca ataaaaggaa aaaacaaact
     8101 gccgagcata agagagttta aaaaaggtct gaacatttat tctatttcct caatgattag
     8161 tcatccttga gggttgtgtt atccatactt tcaggttggc tgattcgcag cagaccattc
     8221 tctccagatt catcttatgc tcgatatttc aacaaattaa gacttttctg aagagggcgg
     8281 ccattgttgt gataaatgaa gtgcctgtcc atcagaaaca gtaacaacta ttttcacccg
     8341 atcttccggg gaaatattta aactcaactt cgtcaaagca ataggttgat tagcaggcaa
     8401 tgagagggtc ttttcttgct tcgtctgact ttgccctgaa ctgccttcgc gcagggacaa
     8461 tatttgtact ctgcacagac aagattgagt aagagtgact tcaggaataa tggtatacac
     8521 atccccttgc tgggtcgtat taaaggttat ctggctggaa agtgccgcaa ggagtaataa
     8581 cgcattcata ttcttctccc gaaaaaaaca gggcttgcgc cctgtttctg taa
//
LOCUS       DAZPLI010000160         8543 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZPLI010000160 DAZPLI010000000
VERSION     DAZPLI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8543
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.161"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="ACHUU3_003542"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="ACHUU3_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR2083526.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHUU3_003543"
     CDS             complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHUU3_003543"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFR2083527.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2464..2744
                     /locus_tag="ACHUU3_003544"
                     /pseudo
     CDS             2464..2744
                     /locus_tag="ACHUU3_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHUU3_003545"
     CDS             complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHUU3_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR2083528.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHUU3_003546"
     CDS             3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHUU3_003546"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR2083529.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6490..6846)
                     /locus_tag="ACHUU3_003547"
     CDS             complement(6490..6846)
                     /locus_tag="ACHUU3_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR2083530.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6850..7266)
                     /locus_tag="ACHUU3_003548"
     CDS             complement(6850..7266)
                     /locus_tag="ACHUU3_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR2083531.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHUU3_003549"
     CDS             complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHUU3_003549"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR2083532.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         2156 a         2137 c         2098 g         2152 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     1741 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     1801 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     1861 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     1921 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     1981 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     2041 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     2101 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     2161 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     2221 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     2281 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagctc acaaaattaa
     2341 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     2401 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     2461 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcagcttct tagtgccgta
     2521 atgatgtttc tggccagcac atcaacatcc gttttcgtcg gtctgattat gtcgggcttg
     2581 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
     2641 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
     2701 agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta gaagcctcca
     2761 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
     2821 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
     2881 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
     2941 gattgccacc ctgcggctgc tgaggctgac cccaaccgcc ctgcggctga ccaccaccga
     3001 tattaccacc tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
     3061 caacgttcac cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac
     3121 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca
     3181 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgttct ttcatctcgc
     3241 cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
     3301 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
     3361 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa
     3421 cgcgcgattg taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa
     3481 caaacctggc cagacattgt tacacaacac tccggataat gcattccaat actgtatatt
     3541 cattcaggtc aatttgtgtc ataattaacc gtttgtgatc gccggtagca ccatgccacc
     3601 gggcaaaaaa gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc
     3661 gcacccataa tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga
     3721 ccgggctttc gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc
     3781 agcgccgtta cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa
     3841 agccggacgt cgatcatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga
     3901 cgtctcataa cccgcgttct acggtgggga caatcaccga aatccacgac tatttgcgtt
     3961 tgttgttcgc ccgcgtcggc gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc
     4021 aaaccgtcag ccagatggtg gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc
     4081 tgctcgcgcc aatcattaaa gagcgcaaag gcgaacacac caaaacgctg gagaacctgg
     4141 caagccaggg ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc
     4201 cgaaactgga actgcaaaag aaacacacta ttgaagtggt ggttgatcgc ttcaaggtgc
     4261 gtgacgatct tacccaacgt cttgcggagt cgtttgaaac cgcgctggag ctttccggtg
     4321 gtacagcggt agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca
     4381 acttcgcctg cccaatttgc ggctacagta tgcgtgaact ggagccgcga ctgttttcgt
     4441 ttaacaaccc ggcaggtgcc tgcccgacct gtgacggcct tggcgtacag caatatttcg
     4501 atcctgaccg cgtgatccaa aatcccgagc tgtcactggc tggcggtgcg atccgtggct
     4561 gggatcgccg caacttctat tatttccaga tgctgaaatc gctggcagat cactataagt
     4621 tcgacgtcga agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg
     4681 gttctggcaa agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccattc
     4741 gtcgtcatcc gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacggaat
     4801 ccagtgcggt acgtgaagaa ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg
     4861 aaggaacgcg tctgcgtcgg gaagcgcgcc acgtgtatgt cgagaatacg ccgctgcctg
     4921 ctatctccga catgagcatc ggtcatgcga tggaattctt caacaatctc aaactcgcag
     4981 gtcagcgggc gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc
     5041 tcgttaacgt cggcctgaat tacctgacac tttcccgctc ggcagaaaca ctttccggcg
     5101 gtgaagccca gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt
     5161 atgtactgga cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgttgggta
     5221 cgcttatcca tctgcgcgat ctcggtaata ccgtgattgt ggtggagcat gacgaagacg
     5281 caattcgcgc cgctgaccat gtgatcgata tcggtcctgg tgcgggtgtt cacggcggtg
     5341 aagtggtcgc agaaggtccg ctggaagcga ttatggcggt gcctgaatcg ttgaccgggc
     5401 agtacatgag cggtaaacgc aagattgaag tgccgaagaa acgcgttccg gcgaatccag
     5461 aaaaagtgct gaagctgaca ggtgcacgcg gcaacaacct gaaggacgtg acgctcacgc
     5521 tgccagtcgg tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga
     5581 ttaacgacac actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac
     5641 cggcaccgta tcgcgatatt caggggctgg agcatttcga taaagtgatc gatatcgacc
     5701 aaagcccaat tggtcgtact ccacgttcta acccggcgac ctataccggc gtgtttacac
     5761 ctgtgcgcga actgtttgcg ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac
     5821 gtttcagctt taacgtccgt ggcgggcgct gcgaagcctg tcagggcgac ggtgtgatca
     5881 aagtggagat gcacttcctg ccggatatct acgtgccgtg cgatcagtgc aaaggtaaac
     5941 gctataaccg tgaaacgctg gaaattaagt acaaaggcaa aaccatccac gaagtgctgg
     6001 atatgaccat cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc
     6061 tgcaaacgtt gatggacgtt ggtctgacgt acattcgcct ggggcagtcc gcaaccacac
     6121 tttctggtgg tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg
     6181 ggcagacgct gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc
     6241 aactcctcga cgtgctgcat aaactgcgcg atcagggcaa taccattgtg gtgattgagc
     6301 acaatctcga cgtgatcaaa accgctgact ggattgtcga cctgggacca gaaggcggca
     6361 gtggcggcgg cgaaatcctc gtctccggta cgccagaaac cgtcgcggag tgcgaagctt
     6421 cgcatacggc acgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc
     6481 cttttccttt cagagttgta ccagcaattt acgcttttct tccggtagta aattcactgc
     6541 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag
     6601 atacacggtg ctccagtgcg ctttattcag atggcggcta ggacgcacat cgctgtgctg
     6661 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
     6721 tacttctttc accatcgcaa acagtacatc ttccactttg atctgcgtcg ctttccagtc
     6781 gttatgcacg ctctgttctg cgcctggttt tgccatgcaa tattgtagca actccgaaat
     6841 ggtcattttt tactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
     6901 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgtaacgg
     6961 caatacaagc gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc
     7021 ctcatagtca tgctcataat ttccgttgtc gggaacggtg cggaggaaaa aacgctccat
     7081 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
     7141 cagcaacaga tgcagtaagc cgatgttaac gcgcggcata tcagccagct gattcagaat
     7201 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
     7261 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
     7321 ataaaggtgt gattaaaaaa acagcattcg ggagagcatc acgctctccc gctctgtcag
     7381 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
     7441 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta
     7501 atatcgttat cggaatcgcc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     7561 tttgtatttt gccctggttt atcgcccgca aagattaccg gattcatgtt ggtggcagga
     7621 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttcgtcgg gctacgacca
     7681 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     7741 tcttttggaa tgctgaattc atcccagcca ttattcattt tttcccagaa cacaggattt
     7801 ttcagataat cttcgctttc tggcgagaag gtttttttgc cgcgccagaa ccccggactg
     7861 gaaaaaagta ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg
     7921 ctattttcaa tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca
     7981 acgttagtcc cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     8041 agagcgaaca ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct
     8101 aaatagtcag tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat
     8161 aacgccagct gccggggaaa ggaaggatta tctgcggttt ttgtgatgaa aagcaggatg
     8221 attatattta acccagtaaa tacgttcctg tctttttttg tataaaaaat gtttattact
     8281 cgatgtaagg atacattaat tttacgttta ttatgatgaa tcctctgcaa atgagcgaaa
     8341 tttgaaagaa catttatctg ctgtacgatt taatgaactt aatgatacag cattacaaaa
     8401 acaatcgaag tttataaaga tgattcctga ttgaccagaa ccctatccta caatggtgac
     8461 taattttatt actcccgatg gagatgatga tatgaatata tcatatgtga atagcaacaa
     8521 aacgacatct ttacctgttg agc
//
LOCUS       DAZPLI010000161         8507 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZPLI010000161 DAZPLI010000000
VERSION     DAZPLI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.162"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHUU3_003550"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHUU3_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR2083533.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHUU3_003551"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHUU3_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR2083534.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHUU3_003552"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHUU3_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR2083535.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLTNWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="ACHUU3_003553"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="ACHUU3_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR2083536.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="ACHUU3_003554"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="ACHUU3_003554"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR2083537.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHUU3_003555"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHUU3_003555"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR2083538.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="ACHUU3_003556"
     CDS             complement(6574..7338)
                     /locus_tag="ACHUU3_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR2083539.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHUU3_003557"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHUU3_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR2083540.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
BASE COUNT         2048 a         2387 c         2197 g         1875 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg acgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggcg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caggagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacatt
//
LOCUS       DAZPLI010000162         8505 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZPLI010000162 DAZPLI010000000
VERSION     DAZPLI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8505
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.163"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..188
                     /locus_tag="ACHUU3_003558"
     CDS             <1..188
                     /locus_tag="ACHUU3_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083541.1"
                     /translation="MLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYALF
                     PIFTASWRATRIKNIGI"
     gene            411..908
                     /gene="ubiC"
                     /locus_tag="ACHUU3_003559"
     CDS             411..908
                     /gene="ubiC"
                     /locus_tag="ACHUU3_003559"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR2083542.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            921..1793
                     /gene="ubiA"
                     /locus_tag="ACHUU3_003560"
     CDS             921..1793
                     /gene="ubiA"
                     /locus_tag="ACHUU3_003560"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR2083543.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1948..4371)
                     /gene="plsB"
                     /locus_tag="ACHUU3_003561"
     CDS             complement(1948..4371)
                     /gene="plsB"
                     /locus_tag="ACHUU3_003561"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR2083544.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4542..4910
                     /gene="dgkA"
                     /locus_tag="ACHUU3_003562"
     CDS             4542..4910
                     /gene="dgkA"
                     /locus_tag="ACHUU3_003562"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR2083545.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5020..5628
                     /gene="lexA"
                     /locus_tag="ACHUU3_003563"
     CDS             5020..5628
                     /gene="lexA"
                     /locus_tag="ACHUU3_003563"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR2083546.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5701..7026
                     /gene="dinF"
                     /locus_tag="ACHUU3_003564"
     CDS             5701..7026
                     /gene="dinF"
                     /locus_tag="ACHUU3_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR2083547.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7142..7351
                     /locus_tag="ACHUU3_003565"
     CDS             7142..7351
                     /locus_tag="ACHUU3_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR2083548.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7393..7908)
                     /gene="zur"
                     /locus_tag="ACHUU3_003566"
     CDS             complement(7393..7908)
                     /gene="zur"
                     /locus_tag="ACHUU3_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR2083549.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8226..8480
                     /gene="yjbL"
                     /locus_tag="ACHUU3_003567"
     CDS             8226..8480
                     /gene="yjbL"
                     /locus_tag="ACHUU3_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR2083550.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
BASE COUNT         1988 a         2033 c         2287 g         2197 t
ORIGIN      
        1 atatgttgaa tcctacaaaa gataccaatt ggaattccac gtgtatctat aaatccagac
       61 ataagatgtt gcctattaat cttactcagg aaacactttt tagctccaaa tctcatggta
      121 aatatgcgct tttcccaatt tttactgcga gttggcgagc tactcgtata aagaatatag
      181 gtatttaagt aaaggaaaac atcaccgttc ctggcatcat gggcggtgat gcccctacgg
      241 ttgccctcgc cagcacgggc atcggcaaag cgtaaggttc aacagcgttt taccacttca
      301 tgcgattgtt gcgtttttgt tgcgcattag atcacttaat ttgctttaca tctcccgtaa
      361 acacttttct gcgatacaat gcctttacgt tatgtaacgg agagttcggc atgtcacacc
      421 ccgcgttaac gcaactgcgt gcgctgcgct attttaaaga gatccctgcc ctggattcgc
      481 aactgctcga ctggctgttg ctggaagatt ccatgacaaa acgttttgaa cagcagggaa
      541 aaacggtgag cgtgacgatg atccgcgaag ggtttgtcga gcagaatgaa atccccgaag
      601 aactgccgct gctgccgaaa gagtctcgtt actggttacg tgaaattttg ttatgtgccg
      661 atggtgaacc gtggcttgcc ggtcgtaccg tcgttcctgt gtcaacgtta agcgggccgg
      721 agctggcgtt acaaaaattg ggtaaaacgc cgctaggacg ctatctgttc acatcatcga
      781 cattaacccg ggactttatt gagataggcc gtgatgccgg actgtggggg cgacgttccc
      841 gcctgcgatt aagcggtaaa ccgctgttgc taacagaact gtttttaccg gcgtcaccgt
      901 tgtactaaga ggaaaaaaat atggagtgga gtctgacgca gaataagctg ctggcgtttc
      961 atcgcttaat gcgtacggat aagccaattg gcgcgttact gctgctctgg ccaacattat
     1021 gggcgttgtg ggtggcgaca ccgggcgttc cccagctctg gatcctggcg gtgtttgtcg
     1081 cgggtgtctg gctgatgcgc gctgccggat gtgtggtgaa tgattatgct gaccgcaagt
     1141 ttgatggtca tgttaagcgc acggcgaacc gaccgcttcc cagcggcgcg gtaacggaga
     1201 aagaggcgcg cgcgctgttt gtagtgctgg tattgatttc atttttactg gtgctgacgc
     1261 tgaatacgat gaccattctg ttgtcgattg ccgctctggc gttggcgtgg gtgtacccgt
     1321 ttatgaagcg gtatacccat ctcccgcaag tggtgctggg cgcggcgttt ggctggtcga
     1381 ttccaatggc ttttgccgct gtgagtgagt cggtgccatt gagttgctgg ttaatgttcc
     1441 tcgccaatat tctctgggcg gtggcttacg acacgcagta tgcaatggtt gaccgtgacg
     1501 atgatgtgaa gattggtatt aaatccaccg caatcctgtt cggccaatac gataaattga
     1561 ttattggtat tttgcagatt ggcgtactgg cactgatggc gatcatcggt gagttaaatg
     1621 gcttaggctg gggatattac tggtcaattc tggtggctgg cgcgctgttt gtttatcaac
     1681 aaaaactgat tgccaaccgc gagcgtgaag cctgctttaa agcatttatg aataataact
     1741 atgttggtct ggtactgttt ttaggactgg caatgagtta ctggcatttc tgatgatgta
     1801 aaaaagccgg atgatcatcc ggctttcttc tgggttgcct gatgcgctgc gcttctcagg
     1861 cctacacaac acatcgcaat ttattgaatt tgcagattat ggaaggccgg ataaggcgtt
     1921 ttcgccgcat ccggcaattc tctctgatta cccttcgccc tgcgtcgcac tctcaatcgt
     1981 caaacgcacg tctgatgtaa tcaactccgc cagcaactga taaaccttca tcgtttctgc
     2041 cggttcggcg tcgccgctat cgctgatata cccttcatca cgcagcgtca gcaccagaga
     2101 actgaacacc gccttgtcga agaactccgg cgcgttgatg ccgtgcagca cggagagacg
     2161 ttgcgcaacg gtgcggctct ctttctccag cgtaccgcgg ttgatcgacg ggttggcact
     2221 caacaaccag aaggtgatgg cataacgttg cagcgtttcg cgcgcgcctg cggccagcag
     2281 ctgtaggggg cgagaatgcg ccgggttgat atgcaactca tcatcttgca gggtaatcag
     2341 cccctgacgt tgcatctcat ttgccagcgc atcaataacg tccggcaact cgtcgcgatc
     2401 ccagcgcagg aacagctccg ctttcagcat tgggtaaagc acattgacgt gctccatcaa
     2461 tacgtcgcgg gagatgtggc gatgctgggt gacaattgcc gccatcagcg aaggcagcac
     2521 caacatatgc gcaatgttgt tgcgatagta ggtcatcagc accgcttgct cgcgcggcag
     2581 aatgatgatg tcgccgatag tgtctttctc gacttcaaac ttgttcattt gcagcgcgtg
     2641 atcgataagc tcgctggcgc tggctgaagg aacggtagag tccgtggagt aaggtacgtt
     2701 gcgcatcaga tccaggtagc agttgagttg ctcggttaac tgctcgcggg tgagtgagcg
     2761 ctgacgtgat gccagaagcg cagtacaaca caggttcatg gcgtttgccg cgcctgcgtt
     2821 gttaatgcgt accatcagat cggcagcaat attattgacc gtcggcgtta accaggccgg
     2881 acgcaccgct tcgatgggat cgatagattc acgccagtct ggtacgtgct ggttaaggta
     2941 ggtcatcaac ggcattggtt caccgaagtt gacgtaaccc tgaccgagat tacgcagctt
     3001 gcttaaaccg cgcagcatct gcggcaggct ctctttctct ttcgtcgcgc cgcgcagttc
     3061 tttggcgtaa gtacccactt ccatgacgtg ctcataaccg atatagatcg gaatcagcgt
     3121 aatcggacgc gtgccgccac gcagcatcgc ctgaatggtc atcgacagcg taccggtttt
     3181 cggatccagc aaacgccccg tgcgggaacg accgccttcc acgaagtact cgacggaata
     3241 accacggctg aacagttcgc cgagatactc acggaaaacg gtggaataaa gtttattgcc
     3301 tttaaaagta cggcgaataa agaacgcccc cagacggcgg aaaatcggcc cggccggcca
     3361 gaagttcagg ttgatcccgg cggcgatatg cggcggcacc agcccctggt ggtacagcac
     3421 gtaagaaagt agcaggtagt ccatgtgact gcggtggcaa ggcacataca ccagctcatg
     3481 accgtcgtgg gccagctggc gaacgcgctc ggcgttatgg acgttgatgc cctgataaag
     3541 tcggttccag gtgaagccca gaatgcggtc agtcaggcgg atcatctcgt aagagaaatt
     3601 cgccgcaatc tcttccatca gcgcaatcgc gttctgctgc gctttttcat gggagatttt
     3661 tttgctgcgc gcttcatctt ctaccgcttt ggcaatggcg cgggaggcga gcagcttatt
     3721 aaacagatcc tgacgagcag gcagacgtgg gcctacggca gccagacgtt gacgggcaaa
     3781 gtgcatacgc gccacgcgcg ccagtttctg tgcgatagtt ttatccgtgc cgtgttcatc
     3841 cgccatacgg cgcagcgaaa ctgacggcga gaaacgcaca aaactgtcgc gaccgagcca
     3901 cagtacggcg aaaaatttct gtacgccgtt aagcatacgc agcggcgggt ttacttcgcc
     3961 tttttcacgc cccggcgcgc gaccaaacat caccgatact ggcaccatct gcacatccag
     4021 atttgggttg ctacggtgca aatcgagata gtcgtggaac agcttaatag actcttcttt
     4081 cggcgtgtaa taggtgaata cacgcggccc gccgtgaatg aacacatagc gcggcagtag
     4141 cgtgccgtcg atttctagcg gctctaacgg gtcaggcaag tcatgagcca gacactgggc
     4201 gcgtaacgtc agcaaatctg ctttcgagtt gtacggtaaa acgtacataa ttggacgaga
     4261 ggtatccagc cccagttccg gggcaggatc tgccggaata gacttgcttt ttaccaggat
     4321 gcttaatggt aaattcagta atttgtagta aattcgtggc cagccggaca taaacgatgt
     4381 aaagcctctg gttaataatg caaatgcgcg gcaaggatag cagaaagtca tgggaaattc
     4441 tgtggtatcc gctcatgttt cgcgcggcgt tacgcaaacc cgaatcatcg gatttaacgg
     4501 tacactgata ttgacgctca taatgtaaaa aggttctttc aatggccaat aataccactg
     4561 gattcacccg aattatcaaa gctgctggct attcctggaa aggtttacgc gctgcatgga
     4621 tcaacgaagc ggcattccgt caggaaggcg tagcggtatt gttggcggtg gtcatcgcct
     4681 gctggctgga tgtggacgca attacccgcg tgctgcttat cagctccgtg atgctggtga
     4741 tgattgtgga aattctcaat agcgccatcg aagcagtggt tgaccgaatt ggctctgaat
     4801 accatgagct ttccggacgc gctaaagata tgggatccgc cgcggtgctg attgcgatta
     4861 tcgtcgccgt gataacctgg tgcatcctgt tatggtcgca ttttggataa cccttccaga
     4921 tttcgataaa tctctggttt attgtgcagt ttatggttcc aaaatcgcct tttgctgtat
     4981 atactcacag cataactgta tatacaccca gggggcggaa tgaaagcgtt aacggccagg
     5041 caacaagagg tgtttgatct catccgtgat cacatcagcc agacaggtat gccgccgacg
     5101 cgtgcggaaa tcgcgcagcg tttggggttc cgttccccaa acgcggctga agaacatctg
     5161 aaggcgctgg cacgcaaagg cgttattgaa attgtttccg gcgcatcacg cgggattcgt
     5221 ctgttgcagg aagaggaaga agggttgccg ctggtaggtc gtgtggctgc cggtgaaccg
     5281 cttctggcgc agcagcatat tgaaggtcat tatcaggtcg atccttcctt gttcaagccg
     5341 aatgctgatt tcctgctgcg cgtcagcggg atgtcgatga aagatatcgg cattatggat
     5401 ggtgacttgc tggcagtgca taaaactcag gatgtacgta acggtcaggt cgttgtcgca
     5461 cgtattgatg acgaagttac cgttaagcgc ctgaaaaaac agggtaataa agtcgaactg
     5521 ttgccagaaa atagcgagtt taaaccaatt gtcgttgacc ttcgtcagca gagcttcacc
     5581 attgaagggc tggcggttgg ggttattcgc aacggcgact ggctgtaaca tatctctgag
     5641 accgcgatgc cgccaggcgt cgcggtttgt ttttcatctc tcttcatcag gcttgtctgc
     5701 atggcattcc tcacttcatc tgataaagca ctctggcatc tcgccttacc catgattttc
     5761 tccaatatca ccgttccgtt gctgggactg gtcgatacgg cggtaattgg tcatcttgat
     5821 agcccggttt atttgggcgg cgtggcggtt ggtgcaacgg cgaccagctt tctctttatg
     5881 ctgttgctgt ttttacgcat gagcaccacc gggctgactg cgcaggctta tggtgccaaa
     5941 aatcctcagg cattagcccg tgcgctggtg caaccgttgc tgttggcgtt gggggctggg
     6001 gcgttaattg cgctgctgcg tacaccgatt atcgatctgg ctctgcatat tgttggcggt
     6061 agtgaagcag tcctggaaca ggcgcggcgc tttcttgaaa tccgctggtt aagcgcaccg
     6121 gcgtcgctgg cgaatctggt attactcggt tggttactcg gcgtgcaata tgcccgtgcg
     6181 ccagtaattt tgttagtggt cggcaatatc ctcaacattg tgctggatgt ctggctagtg
     6241 atggggctgc atatgaacgt gcagggcgcg gctctggcga cggttattgc ggaatatgca
     6301 acattgctga ttggtctgct aatggtgcgt aaaatcctca aactacgcgg aatttccggc
     6361 gaaatgtcga aaactgcctg gcgaggaaac ttccgtcgct tgctggcgct taaccgcgat
     6421 atcatgctgc gttcgctgtt gttgcaactc tgtttcggcg cgatcaccgt acttggcgcg
     6481 cgactgggga gtgacattat cgctgtaaac gcggttctga tgacgctact cacctttacc
     6541 gcctatgcgc tggatggttt tgcctacgcg gttgaagcac attccggtca ggcatacggt
     6601 gcgcgcgacg gtagccagtt gctggatgtc tggcgggcag cgtgccgcca gtcggggatc
     6661 gtagcgttac tattttcggt ggtttatttg ctggcagggg aacacatcat tgcgttgctg
     6721 acgtcgttaa cccagattca gcagctggct gaccgctatc ttatctggca ggtgattttg
     6781 ccgttggttg gcgtctggtg ttatctgctg gacggcatgt ttataggcgc aacgcgtgcc
     6841 gccgaaatgc gtaacagtat ggcggtggcc gccgcaggtt ttgcgctgac gctccttacg
     6901 ctgccgtggc tgggtaatca tggtttgtgg ctggcattaa ccgtctttct ggcgttgcgc
     6961 gggctttctc tggcggctat ctggcggcgt cactggcgta acggtacctg gtttgccgca
     7021 acgtgacggt taaaaattct gaataaatta tcctaagcca aattgctgac tacacttaat
     7081 ctcacgttca gaagaaaagt gaacgtactc tcattcacaa cctaacgatg aggtcttgat
     7141 tatgaataaa gatgaagccg gcggtaactg gaaacagttt aaaggtaaag tgaaagagca
     7201 atggggcaaa ctgaccgatg atgatatgac gatcattgaa ggtaaacgtg atcaactggt
     7261 cggtaaaatc caggaacgtt atggttatca gaaagatcag gcagaaaaag aggtcgtgga
     7321 ttgggaaacc cgcaatgaat atcgctggta attaatccct cctgcccgac gtgtacaagg
     7381 atgtacgccc tcttaacgcg gtttcttttt cacctgcaca gagtgatcat gctggcactg
     7441 ttcaggatga cgacacgctt ccacttccac gcatgccgca cagagcccat gtgcttcaat
     7501 cacattatgc cgcagggcaa accccatttt tgccgccagc gtatgcataa tgtcttccac
     7561 gccttctgca cactcttctt tcaccgcgcc gcagcgatcg catataaaca tggctgacgt
     7621 atgcgtgggc tggtcgaaca gatgacagag cacataactg ttggtggatt ccaccttatg
     7681 cacaaaacct tgctcaagca gaaaatccag cgcgcgataa accgttggcg gcttggcttg
     7741 cggttcagct tcgcgcagta aatcaagcag atcataagcg ctgatagcgc catcctgcag
     7801 actcatcagg cgcaacactt ccaggcgctg tggggtcagg cgcacattac gctgcgcgca
     7861 gattttttca gcctgcgcta ataactcctg cgttgtggtc ttttccatcg ggcacctcaa
     7921 agtgggggtt aagaatgtcc ttactttacc atgttccagg aaaaacaccg agaaccctta
     7981 ttgttgccgt aatgttgatt ttctgttttg taggtaaggt gttatgttgc cttgtcgtac
     8041 cattatcaac acgataataa ttaataattc atttttaaat aaattgcttc aagttaactc
     8101 gctatttatt taactgggtt taaacgcata cctgtagttc agaaattatg agcgattttt
     8161 gatagtgacc agatggcaaa agaattataa ggaggacacg gtacgacaaa aaaggtagat
     8221 gaatcatgtt gaagataata cccggtgcaa ctggatactt taataaaaca ctcaattcaa
     8281 atcaattcga taatgaagat gcaatcaaag ataaattaga taatagaggt tcaatcaaag
     8341 gtaaattaaa taatatatat ggtaaatcta ttgattatgc agcgctgcgt catcgggata
     8401 taattattgc aaaaatagat ttgtttattc aacgaatcac gcataattta tggcatgcac
     8461 gtaaaaaaat gtgtttttaa ttgagcaaat aaatgatctc aaaat
//
LOCUS       DAZPLI010000163         8503 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZPLI010000163 DAZPLI010000000
VERSION     DAZPLI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8503
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.164"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(240..316)
                     /locus_tag="ACHUU3_003568"
     tRNA            complement(240..316)
                     /locus_tag="ACHUU3_003568"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(280..282),aa:Asp,seq:gtc)
     gene            complement(449..1180)
                     /gene="dnaQ"
                     /locus_tag="ACHUU3_003569"
     CDS             complement(449..1180)
                     /gene="dnaQ"
                     /locus_tag="ACHUU3_003569"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR2083551.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1245..1712
                     /gene="rnhA"
                     /locus_tag="ACHUU3_003570"
     CDS             1245..1712
                     /gene="rnhA"
                     /locus_tag="ACHUU3_003570"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR2083552.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1709..2431)
                     /locus_tag="ACHUU3_003571"
     CDS             complement(1709..2431)
                     /locus_tag="ACHUU3_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR2083553.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2465..3220
                     /gene="gloB"
                     /locus_tag="ACHUU3_003572"
     CDS             2465..3220
                     /gene="gloB"
                     /locus_tag="ACHUU3_003572"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR2083554.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3292..4650
                     /gene="mltD"
                     /locus_tag="ACHUU3_003573"
     CDS             3292..4650
                     /gene="mltD"
                     /locus_tag="ACHUU3_003573"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR2083555.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4699..5322)
                     /locus_tag="ACHUU3_003574"
     CDS             complement(4699..5322)
                     /locus_tag="ACHUU3_003574"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR2083556.1"
                     /translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(5326..6126)
                     /locus_tag="ACHUU3_003575"
     CDS             complement(5326..6126)
                     /locus_tag="ACHUU3_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR2083557.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6367..7281
                     /gene="yafC"
                     /locus_tag="ACHUU3_003576"
     CDS             6367..7281
                     /gene="yafC"
                     /locus_tag="ACHUU3_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR2083558.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7278..8081)
                     /gene="dkgB"
                     /locus_tag="ACHUU3_003577"
     CDS             complement(7278..8081)
                     /gene="dkgB"
                     /locus_tag="ACHUU3_003577"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR2083559.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8244..8320)
                     /locus_tag="ACHUU3_003578"
     tRNA            complement(8244..8320)
                     /locus_tag="ACHUU3_003578"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8284..8286),aa:Asp,seq:gtc)
BASE COUNT         2293 a         2032 c         2038 g         2140 t
ORIGIN      
        1 ataacagtat atataaattc attaaatcaa tagaaaaatg aaaatcataa cattcaaata
       61 tatttaaatt gaatgattcg atagtatgat taaatattaa tgatatggtc taccatatgt
      121 gattaacaca tttacagcat aacaaaacca tagaccgaag aaatagcggg atttaaatgc
      181 aaaaaaaccg caaaattaaa aatcttgcgg ttctctgaac tcattttcat gagtgaattt
      241 ggcggaacgg acgggactcg aacccgcgcc cccctgcgtg acaggcaggt attctaaccg
      301 actgaactac cgctccgcgt tgtgttccgt tgggaacgag gcgaatatta cggattgcct
      361 cacccttcgt caacggtttt tctcatcttt tgaatcgttt gctgcaaaaa tcgcccaagc
      421 cgctattttt agcgcctttt acaggtattt atgcccgcca gaggcagctt ccgcccttct
      481 tctccaccag atcaagacgg gcttcatgag ctgcaagctc ttcatctgtc gcaaaaacaa
      541 cgcgtaactt acttgcctga cgtacaatgc gctgaattgt tgcttcacct tgttgctgct
      601 gtgtctctcc ttccatcgca aaagccatcg acgtttgacc accggtcatc gccagataaa
      661 cttccgcaag gatctgtgca tcgagtaatg ccccgtgtag cgttcgttta ctgttatcta
      721 tttcgtagcg ggcacataac gcatcgaggc tgttgcgctt accaggaaac attttcctcg
      781 ccaccgcaag gctatcggtg accttacaga aggtattggt cttcggaata tcgcgcttaa
      841 gcaacgaaaa ctcgtagtcc ataaagccga tatcgaacgc tgcgttatgg atcaccaact
      901 ccgcgccgcg gatatagtcc atgaactcat cggctacttc ggcaaacgtg ggcttatcga
      961 gcaaaaattc atcggcaata ccatgtacgc caaaggcttc cggatccacc agccgatcgg
     1021 gtttgagata aacatggaag ttattgcccg tcaggcgacg gttcaccact tcaacggcac
     1081 caatctcaat gatcttgtgg ccttcatagt gcgcaccaat ctggttcata ccggtggttt
     1141 cggtatcgag aacgatctgg cgtgtaattg cagtgctcat agcggtcatt tatgtcagac
     1201 ttatcgtttt acagttcgat tcaattacag gaagtctacc agagatgctt aaacaggtag
     1261 aaattttcac cgatggttcg tgtctgggca atccaggacc tgggggttac ggcgctattt
     1321 tacgctatcg cggacgcgag aaaaccttta gcgcaggcta cacccgcacc accaacaacc
     1381 gtatggagtt gatggccgct attgtcgcgc tggaggcgtt aaaagaacat tgcgaagtca
     1441 ttttgagtac cgacagccag tatgtccgcc agggtatcac ccagtggatc cataactgga
     1501 aaaaacgtgg ctggaaaacc gcagacaaaa aaccagtaaa aaatgtcgat ctctggcaac
     1561 gtcttgatgc tgccctaggg caacatcaaa tcaaatggga atgggttaaa ggccatgccg
     1621 gacacccgga aaacgaacgc tgtgatgaac tggctcgtgc tgcggcaatg aatcccacac
     1681 tggaagatac gggctaccaa gtcgaagttt aagcttgtgg tttacgacat tgccgggtgg
     1741 ctccaaccgc ctggcgaatt cgtggcttgt ttttactctg tttcatcggg tttagcgtta
     1801 aaggaatagt ccgtttccgg gcaacaataa gttgtaagca accaagcgca ggaatatgcg
     1861 cattcaatag ttttcctccg tgtttgttcc acgggagaac gtggaaacgg ctggcgtgca
     1921 gcacttcaaa attcaacaaa gagagccagt ccagttgccg catcagagta aacatccggc
     1981 tgttataggg cgaggttttt cgcaataccg gcacaagttt gcgtaatccc attaaactaa
     2041 tgggattgaa gccactaatc accagccagc catcatcaat caatacccga tcggtttcac
     2101 gcaataaacg atgcggatcg gtgcaccacg gcaatgtatg tgccagtaga caaacatcaa
     2161 cggatttatc ggcaaaagga agatgaagtg gatccgcctg cacctggacg ggcattcctt
     2221 gcgcagaaac attcacttga tgagaaaccg cgcacgcttc gcaattgatt tctgcgctta
     2281 aattgccaat cttaagcaga tgaaaaccat acattttagt gaaccacggg ttgagctggc
     2341 gctccagcgc cttgcgataa agctctcccc agggcaaatc gccccagcga tcaggagcca
     2401 cgacagtttg agggactctt gccggtttca tcacaacctt ccgtttcaca ctaagaggta
     2461 atctatgaat cttaacagta ttcccgcctt tgatgacaat tacatctggg ttttgaatga
     2521 tgaagcaggt cgctgcctga ttgtcgatcc cggagacgca gagccagtat taaacgccat
     2581 tgccgccaat aactggcaac cggaggccat atttctcacc caccatcatc acgatcacgt
     2641 tggcggcgta aaagaactgg tggaaaagtt tccgcaaatt gtggtgtatg gtccacaaga
     2701 gacacaagat aagggaacaa cacaggtagt caaagatggc gaaactgcct tcgttttggg
     2761 gcatgaattt agtgtaattg ccacaccggg tcacacttta ggacatatct gttacttcag
     2821 taaaccttat ctattttgcg gcgacacgct gttttctggt gggtgtggta ggttgtttga
     2881 agggacagca tcacaaatgt atcaatcact taataagtta agtgcgttac ctgacgatac
     2941 attggtatgt tgtgctcatg aatatacgtt atcaaatatg aagtttgctt tgagtattct
     3001 tccgcacgat ttgtccataa atgattatta tcgtaaagtt aaggagttac gggcaaaaaa
     3061 tcaaataaca ctacccgtaa ttctgaaaaa tgagcggcaa attaatgttt ttttaagaac
     3121 ggaagatatt gatttaatta atgtaattaa tgaagaaaca ttattgcaac aacctgaaga
     3181 gcgttttgca tggttaaggt caaagaaaga taggttctga taaaactttc ttgtcatcgg
     3241 ctccgttcgc cgttatgatc ggtcgtcttt taagcaacta ttgacacaca catgaaggca
     3301 aaagcgatat tactcgcctc tgtcctgctc gtgggttgcc agagtaccgg caacgttcaa
     3361 cagcacgcac agagcctttc tgcagctggt caaggggaag cagcaaagtt tacaagtcag
     3421 gcacgatgga tggacgatgg gacgtctatc gcgccagatg gtgacttgtg ggctttcatt
     3481 ggcgacgagc taaagatggg aattccggaa aatgaccgga ttcgcgaaca gaaacagaaa
     3541 tatttacgca ataagagcta tctccacgat gtaactttac gggcagagcc gtatatgtac
     3601 tggatagcag ggcaagttaa aaaacgtaac atgcctatgg aactggtact actacccata
     3661 gtggagagcg cttttgatcc tcacgcaacg tctggcgcca atgccgcagg catctggcag
     3721 atcattccga gcacggggcg caattatggt ttgaaacaga cccgcaatta tgacgcgcgt
     3781 cgcgatgttg ttgcttcaac aactgccgcg ctgaatatga tgcagcgtct gaacaaaatg
     3841 tttgacggcg actggcttct gaccgtagcg gcttataaca gcggcgaagg tcgggtcatg
     3901 aaggcaatta aaacgaacaa agcgcgtggg aaatccacgg acttctggtc gttaccgttg
     3961 ccgcaggaaa cgaagcagta cgtgcctaaa atgctggcat tgagtgatat tctcaaaaac
     4021 agcaagcgtt atggcgtacg tctgccaacg accgatgaaa gccgtgctct ggcgcgtgtg
     4081 cacctgagta gcccggttga aatggcgaag gttgcagata tggcggggat ttccgtcagc
     4141 aagctgaaga cattcaacgc tggcgtgaaa ggctccacgc tgggcgcaag tgggccgcag
     4201 tacgtgatgg tgccaaagaa gcatgcagat caactgcgtg aatctctcgc ttcaggcgaa
     4261 attgctgctg tacagtcgac gctggttgcc gacaatacgc cgcttaacag ccgtgtttac
     4321 accgtacgct ctggcgacac gctttcaagc atcgcttcac gtctcggcgt aagcaccaaa
     4381 gatttgcagc agtggaacaa actgcgcgga tctaagctga agccaggcca aagtttgacg
     4441 attggtgcag gcagtagcgc acagcggttg gcaaacaaca gcgatagcat tacgtatcgt
     4501 gtgcgcaaag gcgattcgct ttcaagcatt gctaaacgcc acggcgtgaa catcaaagat
     4561 gtgatgcgct ggaacagcga cactgcgaat ctgcaaccag gcgataagct gacgttgttt
     4621 gtgaaaaaca acaacatgcc agattcctga caaaccagat aataaaaaag gcaccgattc
     4681 ccccggtgcc ttttttattt atgccgcttt atgtgcttct accatgatga tatcactggt
     4741 gaaagagcca tcagtctgca aggaaaaata cgttctcacc tctgtcgatg cgctctgttg
     4801 gtaaatgcga atagcgtcta ctaacgcttc tggcgtacgc attctcacga cccatgaaga
     4861 aaattccagc ggtaacttat ctgtaattaa attatcaact atcagattgg cttcattgat
     4921 taacgtcaac cacgcaccgc tggcgtagtt tcgtacgtga gaggtatcgc gtaatgcttc
     4981 taccgcctgt aaccagatgt cgcgcactgg gtgacccgga gacattacat ccatcacaat
     5041 cagcctaccg ccaggtttca atatcctatt cacttctcgc agtgctgcac caacatcatg
     5101 ccagtgatgg gcagaataac ggctgataac aatatcaaat gcgttatcgg caaatggcag
     5161 acttgcggca tatccctggc gggtggtgat atttttcagt tgccgggctt cggcagcttg
     5221 tgccacgaca tccagcatgt gggcagataa gtcatacgcc accaccgcgc tcacgttttg
     5281 cgcagcgcca aacgttggtc tgccctgcgg cagacctgac ataccttatt tatcaggctt
     5341 gccgggactg aattcaacga gtagcggatt gtgatcggaa gcgcgcgtaa ccagtacaga
     5401 agcttcactg acgttcagac cacggtagaa aacaaaatcg agcgggcgac caaacgcccg
     5461 acggcgctga tcatcggtaa aacgcacctg gcgcagcgac atttcccgcg caaagcgata
     5521 taacgcgttc atccttctac ggctccaggc attgaaatct cccgccataa tgaccgggcc
     5581 gctgtggtga gctatctgat caccaatagg aagtaactgc ttactataga catccacgcc
     5641 cagactgaag ttgacggcgt gtatattaac caccatcaac aggcgggtgt caggtaatgg
     5701 atagaccgtc accagtgccg acttcgccag acgcaaaatg ggttctcgtt cacgtaacgg
     5761 gcagcaatac actggatgtg ccgccgaaag ggtcattacg ccagaaggat gttgtggcag
     5821 cacgaaagcg ggtacctgat cggcggcaag atagttagcg gtcgcaaact gtaccaactc
     5881 tggcgttgtt tgcgcttcct gcaataacac cagatgtgcg tctttgccgt aattctttaa
     5941 taccgacaac cattcagcgc gttgctgttt gtatatgttc cacaccagga tccgaatacg
     6001 ctcttcggta cttaacggtt ctccgggtgg taatgcctgg ccgatgctcg caaaagaccc
     6061 cggcggtaag atcctctccg caggttgtcc ggcaacatag cgcatggcat aggtgttttt
     6121 tcgcactttt gactttgaaa cctctattac gtcaaccctt ccgcactcgg gagggttcta
     6181 ctgttatagg gattttagct cactctttca acgagcaatt gtatattgtt atgtaagcaa
     6241 gtgcttacac cggtcagaaa tttgctcatt attgagaaca ttaaatcgga tgacatatca
     6301 cgcatcctga tggtttaatg acaaataaat tcaaaatatc tcatctattt tgcaacagga
     6361 gcaaaaatga aagccacgtc ggaagaactc gccatttttg tttcggtcgt cgaaagcggc
     6421 agctttagcc gggcagcgga acaattaggg caagcaaact cagcggtaag ccgggcggtg
     6481 aaaaagctgg aaatgaaact tggcgttagc ctgcttaatc ggaccacgcg gcaacttagc
     6541 ctgacggaag aaggcgagcg ttattttcgt cgcgtacagt caattttgca ggagatggca
     6601 gcggcagaat cagaaattat ggagacgcgt aatacaccgc gtggactgtt acggatcgat
     6661 gccgcaactc cagtggtgct gcactttctg atgccgttaa ttaagccttt ccgtgaacgc
     6721 tatccggaag tcactttatc gctagtctcc tccgaaacga ttattaattt gatcgaaaga
     6781 aaagtggatg tcgcgatacg cgctggtacg ttaacggact ccagcttacg tgccaggccg
     6841 ttatttaaca gttatcgaaa aattatcgcc tcccccgatt atatttcccg ctacgggaag
     6901 ccagaaacga tcgacgattt aaagcaacat gtttgcctgg gattcactga acccgcttcc
     6961 ctcaatacct ggccgatagc ctgtagtgat ggacaattac atgaggtgaa gtacggtttg
     7021 tcatccaata gtggggaaac actgaaacag ctttgcctga gtggcaacgg gattgcgtgt
     7081 ttgtccgact acatgatcga caaagaaatc gctcgcggag aattggtgga gttaatggca
     7141 gataaagtgt tgccagtgga aatgccattc agtgccgtct attacagcga ccgtgcggta
     7201 agtacgcgca tccgggcttt tatcgatttc cttagcgagc atgtaaaaac agctcccgga
     7261 ggagctgtca gagaggctta atcccattca ggagccagac cttccgggct aaccaggcgg
     7321 tcgttgcaat ccagtgcggc gatcgctttt ttatcttcgg catcaagctg taaattttgt
     7381 gccttaagat tactttccag gtttttacgt ttagtagaag aaggaattac tgagtaacct
     7441 tcccccatag cccacgccag aatcacttgt gccggagtcg cattgtgttt agctgcaata
     7501 cgagcaataa cctcatcttt cagtgcctta ccataagcca gcgtcatata ggaagtgata
     7561 tgaataccgt gctgtttagc ccaggcaacc actttacggt tttgcagata aggagagagt
     7621 tcaatctggt tggtagcgat gttttcagcg ccaacagcag caatcgcttt ttccatcaac
     7681 gggatcgtga agttggaaat accgatctca cgtgtcagac cttgtttttt ggcctccagc
     7741 agcgcctgca taaactcttc aacagagact tcatcgtttg gtgacggcca gtggattagc
     7801 gtcagatcaa cataatcggt acgcaatttt tgcaggctct cttttagact cgggatcaat
     7861 ttgtctttgc tgagattttc aatccagatt ttagtggtga tgtagagttc atgacgtggc
     7921 acgccacttt ctgcaatcgc ctgacctact gcggcttcgt tatcatagat ttgtgcagta
     7981 tcaattgcgc gataaccaag ttcaagcgcc gttttcacag atgaaataac aacgtcgtct
     8041 ttgagacgga aagtacctaa accaaatgca gggatagcca taatatgcct cttttaatgc
     8101 tattttcgtt aactgaggtg attatgggct tcagactgtt gcggaaaaag agcataaaat
     8161 gcagaggatt tttgcgattc tggcaataat agatatacag acgaaaaaaa acctcgagcg
     8221 ttaactcgag gcttcttaat aagtggcgga acggacggga ctcgaacccg cgaccccctg
     8281 cgtgacaggc aggtattcta accgactgaa ctaccgctcc accgaattct tttacaacca
     8341 ccggttttat gaccggctta ctgcttaatt tgatgcctgg cagtttatga atcacttcgt
     8401 gattcaccct tcgggccgtt gcttcgcaac gttcaaaagc tttcgctttt gtcctactct
     8461 cttatcgagt agtgaactgc ctcaaaggtc agtgccattc ttc
//
LOCUS       DAZPLI010000164         8410 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZPLI010000164 DAZPLI010000000
VERSION     DAZPLI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8410
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.165"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            118..378
                     /gene="repA"
                     /locus_tag="ACHUU3_003579"
     CDS             118..378
                     /gene="repA"
                     /locus_tag="ACHUU3_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR2083560.1"
                     /translation="MSQIENAVTSSPKRIYRKGNPLTGAEKQRISVSRKKGTHKAINV
                     FIQSELKDDLTQLCKDSGLTQKEMIEHWILKEKAAMTDANRK"
     gene            complement(419..550)
                     /locus_tag="ACHUU3_003580"
     CDS             complement(419..550)
                     /locus_tag="ACHUU3_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HFR2083561.1"
                     /translation="MFFVAKHRKIIGVFAFLAPVNPHQNQFPATLRRGQPQNSLNDQ"
     gene            611..685
                     /gene="tap"
                     /locus_tag="ACHUU3_003581"
     CDS             611..685
                     /gene="tap"
                     /locus_tag="ACHUU3_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR2083562.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            678..1535
                     /gene="repA"
                     /locus_tag="ACHUU3_003582"
     CDS             678..1535
                     /gene="repA"
                     /locus_tag="ACHUU3_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR2083563.1"
                     /translation="MTDLHQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSVLFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDAGRERQDIVTLVKRQLTREIAEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            2448..2585
                     /locus_tag="ACHUU3_003583"
                     /pseudo
     CDS             2448..2585
                     /locus_tag="ACHUU3_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            2605..2868
                     /locus_tag="ACHUU3_003584"
     CDS             2605..2868
                     /locus_tag="ACHUU3_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HFR2083564.1"
                     /translation="MNSTTHYENANFLRELAESLPRIFPEGSTDKSALLQRLANEELA
                     RAEYDEQIRAKVAAARADKRPGMSSVQLRQQLQGRYQELRNEL"
     gene            2858..3157
                     /locus_tag="ACHUU3_003585"
     CDS             2858..3157
                     /locus_tag="ACHUU3_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR2083565.1"
                     /translation="MSYEIEWRPGAIEDITALFEYLAENASLWDAGNVTERLITATDR
                     LADYPRLYETDERYGEGVRRISLLGQHVLYEVNDKARRVNILAVVGQRQNPRFIL"
     gene            3515..3790
                     /locus_tag="ACHUU3_003586"
     CDS             3515..3790
                     /locus_tag="ACHUU3_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFR2083566.1"
                     /translation="MKYSKPISSPSCFGERISTELEFVRKICRAEGETEADDDKLVDE
                     FRRLAEHPGGSDLIYYPRDDREDSHEGIVKEVKEWRAKNGKPGFKQG"
     gene            complement(3868..5055)
                     /locus_tag="ACHUU3_003587"
     CDS             complement(3868..5055)
                     /locus_tag="ACHUU3_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFR2083567.1"
                     /translation="MLLGSQFVFNIGFYAVVPFLALFLRDDMLLSGGLIGLILGLRTF
                     SQQGMFILGGTLADRYGAKAIILAGCVVRVAGFLLLACGASLWPIILGACLTGVGGAL
                     FSPSIEALLARAGTHSQANGKRSRAEWFALFAVCGELGAVIGPVAGGVLSGIGFRHIA
                     LAGAGIFLLALAVLFFGLPADGHTTTTRRRVPWWTPLRQPRFVAFILAYSSWLLSYNQ
                     LYLALPVEIQRSGGREQDLAPLFMLASLLIITLQLPLARFARRMGAVRILPVGFLLLS
                     ASFASVALFAAAPPAESWLRLMPAAGFVTLLTLGQMLLVPAAKDLIPLFAEESTLGAH
                     YGALATAGGCAVLAGNLLLGHLLDQALIPSPQAVYPWLLLALFPLCSAVALRAICRPL
                     AAT"
     gene            complement(5112..5873)
                     /locus_tag="ACHUU3_003588"
     CDS             complement(5112..5873)
                     /locus_tag="ACHUU3_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR2083568.1"
                     /translation="MSICAENITWKAGKKVIVNNVSLRVPRGETVGLLGPNGCGKSSL
                     LRVLAGLRRPDAGRVTLDGQDIARMAKKQLARRVAYVEQHGMTEANMRVRDVVRLGRI
                     PHHSPFSNWRAQDDEAIAAALQRVAMLEKSEQGWLSLSGGERQRVHIARALAQSPSEI
                     LLDEPTNHLDIHHQMQLMQLISELPVTSIVAIHDLNHAAMFCDSLIVMQQGQILASGT
                     PEEILSEALLWDVFRVKTKIEISPYHGKKHIHFIV"
     gene            complement(5873..6910)
                     /locus_tag="ACHUU3_003589"
     CDS             complement(5873..6910)
                     /locus_tag="ACHUU3_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFR2083569.1"
                     /translation="MSVAAIETRRSILLTGWCVLAAIVLALVIAVGVSVGELAIPLQN
                     VFYAISNRTGLTAEPLNRIYESVIWDFRLSRALVAACCGAGLAICGVVLQSLLKNALA
                     EPYVLGVSAGASTGAVSIVVLGLGAGAISLSAGAFAGAFAAFAFVALLTNGARGGNER
                     TILAGVAASQLFNAITAYTISTSASAQQARDVMFWLLGSFSGVRWPEFQLVIVVVLAG
                     LAVCLWYARALDAFTFGDDAAASLGIAVPRVRLILFTTAALITATIVSMAGSIGFVGL
                     VVPHVMRFFFGPLHRTLLIASALAGAILMVLADIASRLLIAPQSLPVGVVTALVGVPF
                     FAVIIYRSRNK"
     gene            complement(6910..7908)
                     /locus_tag="ACHUU3_003590"
     CDS             complement(6910..7908)
                     /locus_tag="ACHUU3_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR2083570.1"
                     /translation="MKKVLCALGLMFTAVSSALATTYPLTIENCGYQETFTRPPERVV
                     ALGQNTVEILLLLGLQKQVVASAFWPTSVLPQLAEQNAKIKTLTVEIPSLESVLAQNP
                     DFVPAQLPLLLGPESKVARREDLATVGVNSYVSPGMCATKKATGDMYGSRQKLWDMTW
                     LYQEISDFSRIFNVEDRGQALIADFKKREADLRQEFGKSKKDLSFVFWFSSASPSADA
                     YVGGKNSASGFIASVLGGHNAITSETEWPTVSWESIITANPDVIVVASLDRNRWALDK
                     AEEKIKFLKSDPAVSQLEAVKKGHIVVMDGQAMNPTIRTLYGAEQVGEQLRKMGLN"
     gene            complement(8058..8216)
                     /locus_tag="ACHUU3_003591"
                     /pseudo
     CDS             complement(8058..8216)
                     /locus_tag="ACHUU3_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(8259..>8410)
                     /locus_tag="ACHUU3_003592"
     CDS             complement(8259..>8410)
                     /locus_tag="ACHUU3_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR2083571.1"
                     /translation="RPPVLNEEQKQKVLERIKSGASISAIAREFRTTRQTILRVRAKQ
                     QTVTV"
BASE COUNT         2028 a         2324 c         2329 g         1729 t
ORIGIN      
        1 aatccccgtt gatttgtgcg ccaacacaga tcttcgtcac aattctcaag tcgctgattt
       61 caaaaaactg tagtatcctc tgcgaaacga tccctgtttg agaattgagg aggcgagatg
      121 tcgcagatcg aaaatgcagt aacttcctca ccgaaacgca tttacagaaa aggtaatccg
      181 ttaaccggag ccgagaaaca acgcatatct gtttcccgga aaaaggggac acataaagcg
      241 attaatgttt tcatccagag tgaactcaaa gatgatttga cacagctttg taaggattcg
      301 ggcttaactc aaaaagaaat gatagagcac tggattttga aagaaaaagc tgcgatgact
      361 gatgcgaata gaaagtaact aagttacttt cttgaacatt tgaattgcgg atgctagatt
      421 actgatcgtt taaggaattt tgtggctggc cacgccgtaa ggtggcaggg aactggttct
      481 gatgtggatt tacaggagcc agaaaagcaa aaaccccgat aatctttcta tgtttggcga
      541 cgaagaagat taccggggct aacaagaaac cgtatagcca acaattcagc tatgcgggga
      601 gtatagtcat atgcccggaa aagttcaaga cttctttctg tgctcgctcc ttctgcgcat
      661 tgtaagtgca ggatggtgtg actgatcttc accaaacgta ttaccgccag gtaaagaacc
      721 cgaatccggt gtttacaccc cgtaaaggtg ccggaacgct gaagttctgc gaaaaactga
      781 tggaaaaggc ggtgggcttc acctcccgtt ttgatttcgc cattcatgtg gcgcatgccc
      841 gttcgcgtgg tttgcgtcgg cgcatgccac cggtgctgcg tcgacgggct attgatgcgc
      901 tgctgcaggg actctgtttt cactatgatc cgctggccaa ccgcgtccag tgctccatca
      961 ccacgctggc cattgagtgc ggactggcga cagagtccgg tgcaggaaaa ctctccatca
     1021 cccgtgccac ccgtgccctg acgttcctgt cagagctggg actgattacc taccagacgg
     1081 aatatgaccc gcttatcggg tgctacattc cgaccgatat cacgttcaca tctgtactgt
     1141 ttgctgccct cgatgtatca gaggaggcag tggccgccgc gcgccgcagc cgtgtggaat
     1201 gggaaaacag acaacgcaaa aagcaggggc tggataccct gggtatggat gaactgatag
     1261 cgaaagcctg gcgttttgtc cgtgagcgtt tccgcagtta ccagacagag cttaagtccc
     1321 ggggaataaa gcgtgcccgt gcgcgtcgtg atgcgggcag ggaacgtcag gatatcgtca
     1381 ccctggtgaa acgacagctg acgcgtgaaa tcgcggaagg gcgcttcact gccagtcgtg
     1441 aggcggtaaa acgtgaagtg gagcgtcgtg tgaaggagcg catgattctg tcacgtaacc
     1501 gcaattacag ccggctggcc acagcttctc cctgaaagtg acctcctcag aataatccgg
     1561 cccgcgccgg aggcattcgc acgcctgaag cccgtcagtg cacaaaaaat cagcaccaca
     1621 tacaaaaaac aacctcacca tccaccttct ggtgcatccg gttccccctg tttttgagac
     1681 aaaacacgcc tcacagacgg ggaattttgc ttatccacat taaactgcaa gggacttccc
     1741 gataaggtta caaccgttca tctcataaag cgccagccgc cagtcttaca gggtgcaatg
     1801 tatcttttaa acacctgttt atatctcctt taaactactt aattacattc atttaaaaag
     1861 aaaacttatt cagtgcctgt cctgtggaca gacagatatg cacctcccac cgcaagcggc
     1921 gggccccgac cggagccact ttagttacaa cacacaaaca caaccaccag aaaaaactcc
     1981 ggtccggcgc agaactgaaa ccacaaagcc cctccctcat aactgaaaag cgccgccgcc
     2041 ccgcccttcg ggcgggaaca acatcgcttt taattatgaa tgttgtaact atactccgtc
     2101 accgctgtca gtcttctcgc tggaagtatt gagtacacgc tcgtaagcgg ccctgacggc
     2161 ccgctaacgc ggagatacgc cccgacttcg ggtaaaccct cgtcgggacc actccgaccg
     2221 cgcacagaag ctctatcatg gctgaaaacg ggtatggctt agcagggctg gggatgggta
     2281 aggtgaaatc tatcaatcag taccggctta cgccgggctt cggcggtttt actccggtat
     2341 cacatgtaac cacagcgtgc cgccttacat gccgctggcg cggcataagc atatgttggt
     2401 ggctgtgtct gaaaggttat atactctgca taaaacctga caacacgcag attgccaggc
     2461 aaatgagcat tatgcatccg gttatcgcct gcctgtgaaa caatatcacc ggggcgaata
     2521 tcgatactct gactcgttat acccatgcgt gcggcatcaa aaaacctgca attgcccttt
     2581 attgaatgaa agggggagtc aattatgaat agcaccacac attacgaaaa cgccaatttc
     2641 ctgcgcgagc tggccgaaag cctgcctcgt atttttccag aaggcagtac agacaagtct
     2701 gcactattgc aacgcctcgc taatgaagaa ctggcacggg cggaatacga cgaacagatc
     2761 cgagcgaaag ttgccgctgc tcgcgccgat aagcggccgg gaatgagttc cgtgcaactg
     2821 cgccagcaac tacagggacg ttatcaggaa ctgcgcaatg agttatgaaa tagaatggcg
     2881 accaggtgcg attgaggata taacggcgtt gtttgaatat ctggccgaga atgcatctct
     2941 ttgggatgca gggaacgtga cagagcgtct gatcactgca accgatcgtc tggcagatta
     3001 ccccaggctg tatgaaacag atgaacgcta tggcgaaggg gttcgccgta ttagcttact
     3061 tgggcagcat gtactttatg aagtgaatga taaggcccgt cgtgtcaata tcctggcggt
     3121 tgtaggccag cgtcagaatc cccgattcat tctttgagtg cccggcttga tgcgggctta
     3181 ccaggaatca cggtttggga gccgcctgga gaaatgaaat tttcccatgc taagagtatt
     3241 gagttgaact agtagaattg acaattcaaa aactgagaac atgaagaaac cctatacgtc
     3301 tgtcatactc gctcgtaagc ggccctgaag gctcgcttac tcgacatccg ttagttataa
     3361 cgtcagaaga attatcgagc tttactcaag atcttcttcg tcaatctcca gccatccacg
     3421 ataagctgac tctgtatctt tcacatcgtt ttgagtcata tcagcattgc gtagaaaatt
     3481 aaaagctgca gcacaaaatt gatcttccag gtatttgaaa tactccaaac ctatatccag
     3541 ccccagctgc tttggcgaga gaatatcaac tgaactggag ttcgtcagaa aaatctgccg
     3601 ggctgaaggt gaaactgagg ctgatgacga taaattagtg gatgaatttc gtcgattagc
     3661 tgaacaccct ggtggttctg atcttattta ttatccaagg gatgaccgtg aggacagtca
     3721 tgaaggtatc gtaaaagaag tgaaggaatg gcgagccaaa aatggcaagc cggggtttaa
     3781 acagggctga ggatctttgc taaaggatta gctgaaaata tggatactag ctgttcactt
     3841 ccagagcggg ctgttacccg ctctgcgtca tgttgccgcc agcgggcgac agatcgcccg
     3901 cagggcgacg gcgctgcaca gcggaaacag cgccagcaga agccagggat aaaccgcctg
     3961 cggcgacgga atgagcgcct ggtcaagtaa atgaccgagc agtaaattcc cggctaacac
     4021 cgcgcaccct cctgcagtgg cgagcgctcc gtagtgggca cccagcgtcg actcctcggc
     4081 gaacagcggg atcagatcct tggctgcggg cactaacagc atctggccga gggtgagcag
     4141 cgtgacaaaa ccagccgccg gcattaaccg cagccaactt tcggcgggtg gggcggcggc
     4201 gaataacgcc acgctggcaa aggaagccga cagcagcaga aagcccaccg gcaggatgcg
     4261 cacggcgccc attcgccggg cgaagcgggc gagagggagc tggagggtga taatcaacag
     4321 cgaggccagc ataaagagcg gcgccagatc ctgctcgcgg ccgccggaac gttggatctc
     4381 caccggcagg gcgaggtaca gttggttgta gctcaacagc catgagctgt aggcgagaat
     4441 aaacgcgaca aagcgcggct ggcgcaaagg cgtccaccat gggactcgtc tgcgcgttgt
     4501 tgtcgtgtgc ccgtcggcgg gcagaccgaa aaagagcacc gccagcgcca gaagaaatat
     4561 tcccgcgccg gcgagggcga tatgccgaaa gccgatcccg ctcagcacgc ctccggccac
     4621 cgggccgatc accgctccca gttcaccgca taccgcaaac agcgcaaacc actcggcgcg
     4681 gctgcgtttc ccgttggcct ggctgtgggt accggcccgc gccagcaggg cctctatcga
     4741 cggggagaac agggcgccgc cgacgccggt caggcaggcg ccaaggataa tgggccacag
     4801 cgacgccccg cacgccagca ggagaaaacc ggcgacgcga acgacgcagc cggcgagaat
     4861 gatcgcctta gcgccatagc ggtcggccag cgtcccgccg aggataaaca tcccctgttg
     4921 ggagaaggta cgcagcccga ggatcaggcc gataagtccg cctgagagca gcatgtcgtc
     4981 gcgcaggaag agggcgagga aaggaacgac cgcgtaaaag ccaatattaa aaacgaactg
     5041 gcttcccagt aacaccggag gccagagggt tgccgcaggg cgcagggaga aaagaggcat
     5101 atccgcccaa cctaaacgat gaaatgaatg tgctttttgc cgtggtaagg ggagatctcg
     5161 attttggttt tcacccggaa aacgtcccac agcagcgctt cggacaaaat ctcctccggc
     5221 gtcccgctgg cgaggatctg cccctgctgc atcacgatca gcgaatcgca gaacatcgcg
     5281 gcatggttaa gatcgtgaat ggccacaatg ctggttaccg gcagctcgct gatcaactgc
     5341 attaactgca tctgatggtg tatatccaga tggttggtcg gctcatccag caggatttcg
     5401 ctcgggctct gcgccagcgc gcgggcgata tgcacccgct gccgctcgcc gccggagagg
     5461 cttaaccatc cctgttcgct tttctccagc atcgctaccc gctgcagcgc ggcggcaatc
     5521 gcttcgtcat cctgagctct ccagtttgag aacggagagt ggtggggaat gcgtcccagg
     5581 cgcacgacgt cgcgcacccg catattggcc tcggtcatgc cgtgttgctc gacgtaagcc
     5641 acgcggcggg cgagctgctt tttcgccatc cgggcgatat cctggccgtc gagggtgacg
     5701 cgacctgcat ccgggcggcg caggcccgcc agaacgcgca gcagcgagga tttgccgcag
     5761 ccgttgggcc ccagcagtcc gaccgtttcg ccgcgcggca cccgcagcga gacattattg
     5821 acgatgacct ttttgcctgc cttccaggtg atattttcag cgcaaatact catcacttat
     5881 tccttgagcg gtagataatc acggcaaaga aaggcacccc aaccagggcg gttactaccc
     5941 caacgggcag actttgcggg gcaatcagca ggcgcgacgc aatgtccgcc agtaccatca
     6001 atatcgcccc tgccagcgcg ctggcgatca gcaaagttcg gtgcagtggg ccaaagaaga
     6061 aacgcatcac gtgcggcacc accaggccaa caaagccgat tgagccggcc atgctgacga
     6121 tggtggcggt gatcagcgcc gcagtggtga agaggatcag gcgcacgcgc ggcacagcaa
     6181 tccccagcga ggcggcggca tcgtcgccga aggtgaacgc gtccagcgcc cgggcatacc
     6241 acaggcagac cgccagaccc gcgagcacca ccacgatgac caactggaat tcgggccagc
     6301 ggacgccgct gaagctgccc agcagccaga acatcacatc gcgcgcctgc tgtgcgctgg
     6361 cggaggtgct gatggtatag gccgtgatgg cgttaaataa ctgcgaggcg gcgacgcccg
     6421 caagaatcgt acgctcattg ccgccgcgcg cgccgttagt cagcaaggcg acaaaggcga
     6481 aggcggcgaa ggccccggca aaggcgcctg ccgagaggga aattgcgcca gcgccaaggc
     6541 ccagcaccac gatcgacacc gcgccggtgg aggccccggc ggagacgccg aggacgtagg
     6601 gttcggcgag ggcattcttc agcaagcttt gcagcacgac accgcagata gcgaggccgg
     6661 cgccgcagca ggcggcgacc agcgcgcggc tgaggcggaa atcccagata acgctctcat
     6721 agatacggtt gagcggctca gcggttaagc cggttctgtt gctgatagca taaaagacat
     6781 tctgcagcgg gatggccagc tcgccaacgc tgacgcccac agcgatcacc agcgccagca
     6841 cgatcgctgc cagtacacac cagccggtga gcagtatgct gcggcgcgtc tcaatcgcgg
     6901 cgacgctcat cagttcagcc ccatttttct cagctgctcg cccacctgct ccgcgccgta
     6961 aagcgtgcga atggtcggat tcatcgcctg accgtccatg accacgatat ggcctttttt
     7021 caccgcctcc agctggctga cggcgggatc gcttttgagg aatttgatct tttcctcggc
     7081 cttatccagc gcccagcggt tacgatccag gctggcgacc acgatcacat ccgggttggc
     7141 ggtaataatg ctctcccagc tcaccgtggg ccactcggtt tcggaggtaa tcgcattatg
     7201 tccgcccagc acgctggcga taaatccgga ggcgctgttt ttgccgccga cataggcatc
     7261 ggccgaaggt gaggcgctgg agaaccagaa gacgaaggag agatcctttt tgcttttacc
     7321 aaactcctgg cgcaggtcgg cttcacgttt tttgaaatcg gcaatcagcg cctgaccacg
     7381 atcttcgacg ttgaaaatgc gggaaaaatc gctaatttcc tgatacagcc aggtcatatc
     7441 ccacagcttt tgccggctgc cgtacatatc gccggtggcc tttttggtgg cgcacatccc
     7501 cggtgagacg tagctgttca cgccgacggt ggcgagatct tcacgcctgg cgactttgct
     7561 ttccggcccc agcagcagcg gcaactgggc gggaacgaaa tcaggatttt gcgccagcac
     7621 cgattccagg ctcggaattt ccaccgtcaa ggttttaatc ttcgcgtttt gctcagccag
     7681 ctgcggtaaa acgctggtcg gccagaaggc gctggcgacc acctgctttt gcagcccgag
     7741 cagcagcaga atttcgaccg tattttgccc cagcgcgacc acgcgctccg gcggccgggt
     7801 gaaggtctcc tgatagccgc agttttcaat cgtcaggggg taggttgttg ccagcgcgga
     7861 actgacggcg gtgaacatca agcctaacgc gcaaaggacc ttcttcatta aacgctatcc
     7921 ttgtaaacag gcgagtggat gaccagcaaa tgggagtaat gcaaatgaaa ctcattattg
     7981 gggcggagtt ttatacaatg ccatatttac agtgtcaatg tgttcttgcc cccttatttc
     8041 cagacagggg ttgtatctta agttaacgtt acccactgac gtcgatattc tcgcggagag
     8101 cgataaccca acgcactgtg cggatggttt tcattgtaat gttcgatcgc cactgcaaga
     8161 ttatgcaatg ccgttcttac attcggtttc ggcatgaacg cgatgtagtc ttccttgggg
     8221 tccgcttgga aaacggaatc ccacgctaag actattttct atacagtaac agtttgctgt
     8281 ttcgctctaa ctctcagaat tgtttgccgt gtggttcgga attccctggc aatagcactt
     8341 atacttgccc cagacttaat gcgttcgagt accttctgtt tttgttcttc atttaatacc
     8401 ggtgggcgac
//
LOCUS       DAZPLI010000165         8409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZPLI010000165 DAZPLI010000000
VERSION     DAZPLI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.166"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            115..726
                     /gene="ruvA"
                     /locus_tag="ACHUU3_003593"
     CDS             115..726
                     /gene="ruvA"
                     /locus_tag="ACHUU3_003593"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR2083572.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            735..1745
                     /gene="ruvB"
                     /locus_tag="ACHUU3_003594"
     CDS             735..1745
                     /gene="ruvB"
                     /locus_tag="ACHUU3_003594"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR2083573.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1892..2677)
                     /gene="znuB"
                     /locus_tag="ACHUU3_003595"
     CDS             complement(1892..2677)
                     /gene="znuB"
                     /locus_tag="ACHUU3_003595"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR2083574.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2674..3429)
                     /gene="znuC"
                     /locus_tag="ACHUU3_003596"
     CDS             complement(2674..3429)
                     /gene="znuC"
                     /locus_tag="ACHUU3_003596"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR2083575.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3508..4440
                     /gene="znuA"
                     /locus_tag="ACHUU3_003597"
     CDS             3508..4440
                     /gene="znuA"
                     /locus_tag="ACHUU3_003597"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR2083576.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4456..5778
                     /gene="mepM"
                     /locus_tag="ACHUU3_003598"
     CDS             4456..5778
                     /gene="mepM"
                     /locus_tag="ACHUU3_003598"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR2083577.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5898..6869
                     /gene="lpxM"
                     /locus_tag="ACHUU3_003599"
                     /gene_synonym="msbB"
     CDS             5898..6869
                     /gene="lpxM"
                     /locus_tag="ACHUU3_003599"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR2083578.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(6957..8409)
                     /locus_tag="ACHUU3_003600"
                     /pseudo
     CDS             complement(6957..8409)
                     /locus_tag="ACHUU3_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
BASE COUNT         2262 a         1983 c         2085 g         2079 t
ORIGIN      
        1 catttttcag ttcatcgaga cacctcgcaa gttttcttca tccttcgctg gatatctatc
       61 cagcattttt ttatcataca gcattatctt tgattcatta cgcaggagcg tcatgtgata
      121 ggcagactca gaggcatcat cattgaaaaa caacccccgc tggtgttaat tgaagtgggc
      181 ggcgtaggct atgaagtgca tatgccgatg acctgttttt atgaactccc tgaagcgggt
      241 caggaagcga tcgttttcac ccactttgtg gtgcgtgaag acgcgcaact gctgtacggt
      301 tttaacaata aacaagagcg cacgttgttt aaagagttaa tcaaaaccaa cggcgtcggc
      361 ccgaagttgg cgctggcgat cctctccgga atgtcagcgc agcagttcgt taatgccgtt
      421 gaacgtgaag aagtgggagc actggtgaaa ctgccgggca ttggtaaaaa aaccgccgag
      481 cgtttaattg tcgaaatgaa agaccgattt aaaggtttgc acggcgatct ctttacgcca
      541 gccgccgatc tggtgctcac gtcgcctgcc agcccggcaa ccgacgatgc tgaacaggaa
      601 gcggttgccg cgctggtggc gctgggctat aaaccacaag aagcaagccg catggtgagc
      661 aaaatcgctc gcactgacgc cagcagtgaa acattaattc gcgaagccct acgcgccgcg
      721 ttatgaggta aaggatgatt gaagcagacc gtctgatttc tgccggtacc actttgccgg
      781 aagatggggc agatcgcgcc attcgcccta aattactgga agagtatgtt ggtcagccgc
      841 aggttcgttc acagatggag attttcatca aagcagcgaa actgcgcggc gatgccctcg
      901 atcatctgtt gatttttggt cctccggggt tgggtaaaac tacgcttgcc aacattgtcg
      961 ccaatgaaat gggcgttaat ttacgcacga cttctggtcc ggtgctggaa aaggcgggcg
     1021 atctggcagc gatgctcact aaccttgaac cgcatgacgt gctgtttatt gatgagatcc
     1081 accgtctatc gccagttgtt gaagaagtgc tgtacccggc aatggaagac taccaactgg
     1141 atatcatgat tggtgaaggt ccggcggcac gctccattaa aattgatttg ccgccgttta
     1201 ccctgattgg tgcaaccacg cgcgcaggtt cgctgacatc accgttgcgc gaccgttttg
     1261 gtattgtgca acgtctggag ttttatcagg tgccggatct gcaatatatc gtcagtcgca
     1321 gcgcacgctt tatggggctt gagatgagtg atgacggcgc gctggaagtt gctcgtcgcg
     1381 ctcgcggtac gccacgtatt gccaaccgtc tgctgcgtcg agtgcgtgat ttcgccgaag
     1441 tgaagcacga tggcaccatc tcggcagata tcgctgctca ggcgctggat atgttgaatg
     1501 tcgatgctga aggtttcgat tatatggacc gcaaattgtt gctggcggta atcgataagt
     1561 tctttggtgg gccggtaggt ctggataacc tggcggcagc cattggcgaa gaacgtgaaa
     1621 ccattgagga tgtgctggag ccttatctga ttcagcaagg ctttttgcag cgtacaccgc
     1681 gtgggcgtat ggcgacgacg cgggcgtgga atcactttgg cataacgccg ccagaaatgc
     1741 cgtaagtcgg attggtcgct cgcaccgcat ccgacattgg cagtgtgcct gatgcgacgc
     1801 tattcgcgtc ttatccggcc taccagagat agcgaaccgt aggtcggata aggcgctcgc
     1861 gccgcatccg acaaatgtgt tcagcgacag attagctggc ctgctttttc atcatactga
     1921 taataaataa cagtgcagca catagcacta ccgacggacc cgccggcgta tcgtaaaacg
     1981 cggaaaaggt taaaccgcca gttactgcca ccatccccac caaaacagcg acaccagcca
     2041 tctgttccgg cgtacgggca aagcgacgcg cagtagctgc aggaataatc agcaacgaag
     2101 taataatcaa cgcaccgaca aatttcatcg ctacaccaat cgtcaatgcc gtcaccagca
     2161 tcaacaacaa tttcacgcgc tgtaatttca caccatcaac aaacgccaga tccgggctaa
     2221 tcgtcatcga cagcaaattg cgccattgcc agaacaaaat agccaccacg atgaccacgc
     2281 caatcgcaat agagatgaga tcttctggcg tcactgccag caaatcaccg aacaggtaag
     2341 ccatcaaatc aacacgaata ttagacatca gactaacgac caccaggccc agcgacaggg
     2401 cactgtgcgc cataatccct aataacgtgt cgatcgccag ctgtggacgc ttctccagcc
     2461 ataccagacc gcccgccagc agcagcgtaa cggcaatcac cgcatagaat ggattcacgt
     2521 ccagcaacaa accaaacgcg acaccaagta atgaggcatg agccagcgta tcaccgaaat
     2581 aagacatacg acgccagact acaaacgaac ccagcggacc cgcggcacag gcgagcatga
     2641 tcccggctaa ccaaccggga aataataatt caatcatgag cgatcatttc cccgacgcaa
     2701 aacaattcgt ccctgtaaat cgtgacgatg gttatgatga tggcgataga tacccagttg
     2761 ttcagcacca cgaggaccaa acattgaaat aaactccgga tgcagggaaa caacttccgg
     2821 tgtgccggaa caacaaatgt ggtgattcag gcacagcact tcatcggttt ttgccatcac
     2881 cagatgcaga tcgtgagaaa ccattaaaac gccacaatcc agttcacgac gcagttggtc
     2941 aataaggtca tataacgcca cctggccatt tacatccacg ccttgagtgg gttcatccag
     3001 cactaataat tgcgggcgat ttaacaatgc tcgcgctaac agtacacgct gcgtttcgcc
     3061 accagagagc ttttgcatcg gtgcgttaat caggtgcccg gcctggacac gtttcagtgc
     3121 aggcaaaata tcttctttat gtgtaccagg gcgtaagcgt aaaaaacggt ttacggtcag
     3181 tggcaacgtg gtgtcgagat acagcttctg cggtacatag ccgatgcgca gttttccgtt
     3241 gcgcttgata accccttcat cgggtgttac cagcccgagc actacccgta ccagtgtcga
     3301 cttacctgcg ccatttggcc caagtaaagt caaaattttt ccaggtttaa gttccagcga
     3361 cacatcagag aggacgcggc gttggccaaa agaaaccgag acattttcca aggaaaccag
     3421 acttgtcatg ttaattttag tcttgcagta gtcatgaaat gttataatat cacacttctc
     3481 atattcatta cgatgattgg tcgcattatg ttacataaaa aaacgcttct tttcgcagca
     3541 ttatccgccg ctctctgggg aggtgcaaca caggccgcag atgccgccgt tgtcgcttcg
     3601 cttaaacccg ttgggttcat cgcttctgcc attgctgatg gggtaacaga aacagaggtt
     3661 ttacttcctg acggcgcttc agaacatgat tattcactgc gcccatcgga tgtaaaacgc
     3721 ttacagaacg cggacttagt cgtttgggtt ggcccggaga tggaagcgtt tatgcaaaaa
     3781 ccggtaagca aattaccagg agcgaagcag gtaacgattg cgcagcttga agatgtgaaa
     3841 ccgctactga tgaaaagtat tcacggcgat gatgatgatc acgaccacgc ggaaaaaagt
     3901 gacgaagatc accatcacgg cgatttcaac atgcatcttt ggctttcccc agagatagcg
     3961 cgggctacag cggttgcaat ccatggaaaa ttagtggaac ttatgccgca aagtcgagcc
     4021 aaacttgacg ccaacctgaa ggattttgag gcacaattag cctcaaccga aacgcaggtt
     4081 ggtaacgagc tcgcgccgct caaggggaaa ggttatttcg tttttcacga tgcttacggc
     4141 tatttcgaaa aacagttcgg actgacaccg cttggtcatt ttaccgttaa ccctgagatt
     4201 caacctggcg cgcagcgttt acatgaaata agaacacagt tggttgagca aaaagcaacc
     4261 tgcgtttttg ctgagccaca gttcaggcca gcggtcgttg agagcgtcgc acgagggaca
     4321 tccgttcgta tgggaacgtt ggatcccctg gggacgaata tcaaactggg taaaacaagc
     4381 tattcagaat tcctgagtca attagccaac cagtatgcga gctgcctgaa aggagattaa
     4441 tgaggaagtg attacgtgca acagatagcc cgctctgtcg ccctggcgtt taataattta
     4501 ccgcgaccac accgcgttat gttggggtcg ctcaccgttc ttactctggc cgtcgctgtc
     4561 tggcggccct atgtttatca ccgcgatgcc acgccaattg tcaaaaccat tgagctggaa
     4621 cagaacgaaa ttcgttcgct cttacctgaa gccagtgagc cgattgatca agctgcacaa
     4681 gaagatgaag ccattcccca ggacgaactg gatgacaaaa tcgccggtga agcgggcgtg
     4741 catgaatatg ttgtttccac tggcgatacg ctaagcagca ttctcaatca gtatggtatt
     4801 gatatgggtg atatcaccca actggctgcg gccgacaaag aattgcgtaa cctgaaaatc
     4861 ggtcaacaac tctcctggac attaaccgcg gacggcgaac tgcagcgcct cacctgggaa
     4921 gtgtctcgtc gtgaaacccg aacctatgac cgtactgccg ctaacggttt taaaatgacc
     4981 agcgaaatgc agcaaggaga gtgggttaac aatctgctga aaggtaccgt cggggggagc
     5041 tttgttgcca gcgccagaaa cgccggttta accagcgccg aagtgagcgc agtgattaaa
     5101 gccatgcagt ggcaaatgga tttccgcaaa ctgaaaaaag gcgatgaatt tgcggtgtta
     5161 atgtctcgag aaatgcttga tggtaaacgt gagcaaagcc agctgctggg cgtacgtttg
     5221 cgttcagaag gtaaagatta ttacgctatc cgcgctgaag atggcaaatt ctacgatcgt
     5281 aacggtactg gtctggcgaa aggattcttg cgattcccga cggcgaaaca gttccgtatc
     5341 tcgtctaact ttaacccgcg tcgtactaat ccggtgaccg gtcgcgttgc agcacacaga
     5401 ggtgttgatt tcgccatgcc gcagggtacg ccagtgcttt cagtgggtga cggtgaagtg
     5461 gtggttgcca aacgcagtgg cgcagcaggt tattatgtgg ctattcgtca tggtcgcagc
     5521 tacaccacgc gttatatgca cttgcgcaag attctggtga aaccgggaca gaaggtgaaa
     5581 cgtggcgacc gtatcgcgct ttccggtaat accggacgtt caaccgggcc gcatctgcac
     5641 tatgaagtat ggataaacca gcaggccgta aacccgctga cggcaaaact gccgcgtacc
     5701 gaagggctga ccggctccga tcgtcgcgaa ttcctggcgc aggccaaaga gattgtgccg
     5761 cagctacggt ttgattaatt aacatccatt cgcagccggt acgcagtcag tatcggcttt
     5821 ttttatttgg tgcggggcaa gttgcgccgc tacactatca ccagattgat ttttgcctta
     5881 tccgaaactg gaaaagcatg gaaacgaaaa aaaataatag cgaatacatt cctgagtttg
     5941 ataaatcctt tcgccacccg cgctactggg gagcatggct gggtgtagca gcgatggcgg
     6001 gtatcgcttt aacgccgcca aagttccgtg atcccattct ggcacggctg ggacgttttg
     6061 ccggacgact gggaaaaagc tcacgccgtc gtgcgttaat caatctgtcg ctctgctttc
     6121 cagaacgtag tgaagctgaa cgcgaagcga ttgttgatga gatgtttgcc accgcgccgc
     6181 aagcgatggc aatgatggct gagttggcaa tacgcggacc ggagaaaatt cagccgcgcg
     6241 ttgactggca agggctggag atcatcgaag agatgaggcg taataacgag aaagttatct
     6301 ttctggtgcc gcacggttgg gccgtcgata ttcctgccat gctgatggcc tcgcaagggc
     6361 agaaaatggc agcgatgttc cataatcagg gcaacccggt ttttgattat gtctggaaca
     6421 cggtgcgtcg tcgctttggc ggtcgtctgc atgcgagaaa tgacggtatt aaaccattca
     6481 tccagtcggt acgtcagggg tactggggat attatttacc cgatcaggat catggcccag
     6541 agcacagcga atttgtggat ttctttgcca cctataaagc gacgttgccc gcgattggtc
     6601 gtttgatgaa agtgtgccgt gcgcgcgttg ttccgctgtt tccgatttat gatggcaaga
     6661 cgcaccgtct gacgattcag gtgcgcccac cgatggatga tctgttagag gcggatgatc
     6721 atacgattgc gcggcggatg aatgaagaag tcgagatttt tgttggtccg cgaccagaac
     6781 aatacacctg gatattaaaa ttgctgaaaa ctcgcaaacc gggcgaaatc caaccgtata
     6841 agcgtaaaga tctttatcct atcaaataaa aaaagcctct caaattattg agaggctgta
     6901 ctcgataaaa aatctcaatg agtgttccca ctattttgag tttttaggtt agtacgctat
     6961 tgagttaatc gtagcgtgat aatttcaaaa ggtttgaact caagattgca atattgattg
     7021 caataaaaac tgtcatctaa cttattttct tgaagatcga catattgaac gtagtgataa
     7081 ttacatactc acgaaagcat ggctgaaatt tctaatccat gactctcata taatcttacg
     7141 attataccgt cgccgtcttc agctaatttt attgtttcga tgataacgct gttagtatcg
     7201 atagcaaaaa atgatagtga agggggtaat tcacccgggt gcggtttgac agggacgacc
     7261 tgtaagggat gattcattat ataggcttga ttaataacac cacccacacg gtaatctcca
     7321 tgatgaggaa ataaggagaa tatgaatttt tgtttaccaa tatctgcaaa attattaatc
     7381 aggaaattat cataattgtc tgtcttaaca acttccccag gtttatgctg cgaacgcagc
     7441 aaagttaatt caagcgtctg gtctttgaca cgatagccat atttgttttt tgctattaaa
     7501 gctatgccag tttctcgatc agaaaggtcg acccagtgat gtgctggtat ttcatctttg
     7561 gcaaaagcga acgaatcatc gctatgcgtt ggtctgtcaa ttacaccgaa ctgaatttga
     7621 cattgtgcaa gagatgtttg tactgacacc gggaatttta ctttgagaga aacattaggt
     7681 tcgtgccaat caataaacgt ttggacatca acgcgtggac tatcctgtgt cagggtaaag
     7741 gctaaacgaa gataagagga attgtagtga taaatcagcg tcatcgtagt tttaggcccg
     7801 ctgatcacag ttgtcatttt ttcagcgagt aaaacatatg attccccatc acgataattt
     7861 gaagcaaaat cccaggccgc gaaaaaacct gcatcttcgt ggtatgcacg gatctcactg
     7921 tgcatatttt cagtaatgta ttctttacca tatcttttat catatagcga aattaaatca
     7981 ccgctttctg aaaaaagaag tttaatgtaa ttattttcaa tactttgaga ttctgccttc
     8041 agaccattta cgatcttatt ctttggaaca atcacagcat aaccataaga gtttactcgt
     8101 gctttgagcc agttgttgtt tatattgatc cattcgttac gagcaaaaca ggtggtatta
     8161 aacaaaatgt gcggatcttt ataagaagaa gtgtcaattt tacttaataa agtagactgc
     8221 gcatcagaaa taattttctc ggtttttgct tctagtttaa gatattcctt ttcagtttca
     8281 tgataaactc ttgaaataca tgagccggga aggatgtcat ggaattgcaa ggtcagcatc
     8341 gctttccata tttcatcaaa ctcactgcga agaattgatg tcatattatt agtaatagtt
     8401 ataaaaaat
//
LOCUS       DAZPLI010000166         8356 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZPLI010000166 DAZPLI010000000
VERSION     DAZPLI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8356
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.167"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            16..1152
                     /locus_tag="ACHUU3_003601"
     CDS             16..1152
                     /locus_tag="ACHUU3_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFR2083579.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
     gene            complement(1194..1964)
                     /gene="yafV"
                     /locus_tag="ACHUU3_003602"
     CDS             complement(1194..1964)
                     /gene="yafV"
                     /locus_tag="ACHUU3_003602"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR2083580.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            2145..2591
                     /gene="ivy"
                     /locus_tag="ACHUU3_003603"
     CDS             2145..2591
                     /gene="ivy"
                     /locus_tag="ACHUU3_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR2083581.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2634..5078)
                     /gene="fadE"
                     /locus_tag="ACHUU3_003604"
     CDS             complement(2634..5078)
                     /gene="fadE"
                     /locus_tag="ACHUU3_003604"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR2083582.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            5318..5896
                     /gene="lpcA"
                     /locus_tag="ACHUU3_003605"
     CDS             5318..5896
                     /gene="lpcA"
                     /locus_tag="ACHUU3_003605"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR2083583.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            6102..6869
                     /locus_tag="ACHUU3_003606"
     CDS             6102..6869
                     /locus_tag="ACHUU3_003606"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR2083584.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(6840..7580)
                     /gene="dpaA"
                     /locus_tag="ACHUU3_003607"
     CDS             complement(6840..7580)
                     /gene="dpaA"
                     /locus_tag="ACHUU3_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR2083585.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(7736..8014)
                     /gene="yafQ"
                     /locus_tag="ACHUU3_003608"
     CDS             complement(7736..8014)
                     /gene="yafQ"
                     /locus_tag="ACHUU3_003608"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFR2083586.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            complement(8017..8277)
                     /gene="dinJ"
                     /locus_tag="ACHUU3_003609"
     CDS             complement(8017..8277)
                     /gene="dinJ"
                     /locus_tag="ACHUU3_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFR2083587.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
BASE COUNT         2126 a         2140 c         2086 g         2004 t
ORIGIN      
        1 aataaggaga acaaaatgga acttaaaaaa ctgatggaac atatttctat tatccccgat
       61 tacagacaag cctggaaagt agaacataaa ttgtcagata ttctactgtt gactatttgt
      121 gccgtcattt ctggtgcaga gggttgggaa gatatagagg attttggtga aacacatctc
      181 gattttttga agcaatatgg tgattttgaa aatggtattc ctgttcacga tactattgcc
      241 agagttgtat cctgtatcag tcctgcaaaa tttcacgagt gctttattaa ctggatgcgt
      301 gactgccatt cttcagatga taaagacgtc attgcaattg atggaaaaac gctccggcac
      361 tcttatgaca agagtcgccg caagggagcg attcatgtca ttagtgcgtt ctcaacaatg
      421 cacagtctgg ttctcgggca gattaagacg gatgagaaat ccaatgagat tacagccatt
      481 cctgaacttc ttaacatgct ggatattaaa ggaaaaatca tcacaactga tgcgatggga
      541 tgccagaaag atattgcaga gaagatacaa aaacagggcg gtgattattt attcgcggta
      601 aaaggaaacc aggggcggct taataaagcc tttgaggaaa aatttccgct gaaagaatta
      661 aataatccag agcatgacag ttacgcaatc agtgaaaaga gtcacggcag agaagaaatc
      721 cgtcttcata ttgtttgcga tgtccctgat gaacttattg atttcacgtt tgaatggaaa
      781 gggctgaaga aattatgcgt ggcagtctcc tttcggtcca taatagcaga acaaaagaaa
      841 gagccagaaa tgacggtcag atattatatc agttctgctg atttaaccgc agagaagttc
      901 gccacagcaa tccgaaacca ctggcacgtg gagaataagc tgcactggcg tctggacgtg
      961 gtaatgaatg aagacgactg caaaataaga agaggaaatg cagcagaatt attttcaggg
     1021 atacggcaca tcgctattaa tattttgacg aatgataagg tattcaaggc agggttaaga
     1081 cgtaagatgc gaaaagcagc tatggacaga aactatctgg cgtcagtcct tgcggagagc
     1141 gggctttcgt agtcttaccc cgactctccc ccagccttaa acacaacccc cactcaccgc
     1201 aacctaaact catccgcatc ctgccatgcc ggaaactttt ctctatattc ccgcaatgcc
     1261 atcatcgaca gctccgcatc aatgcgcgtt gcctgatgcg cgtcggcagt agcgataatc
     1321 tcgccttgtg gattaatcac ccggctgtca ccgcgataat ggcagccgtt gccatcgctg
     1381 ccgacgcgat tgcatcccgc cacatacgcc tgattctcaa tcgcgcgggc cgtcagcaat
     1441 gcctgccagt gcagagagcg cggagcaggc cagttggcaa cgtagatggc gaggtcataa
     1501 tcgttgagat tgcgcgacca caccggaaaa cgtaagtcgt agcacaccaa cggcaaaata
     1561 cgccagccgc gccattccac aatcactcgc gcattgcccg ctttataatg tagatgctca
     1621 tctgccatgc ggaacagatg acgcttatca taaaaatgta ccgtgccgcc cggctcaacc
     1681 agcaaaaagc ggttaaccga accagactcc gtttgtaatg caacactgcc tgcaatcagc
     1741 gcattgcact gctgcgcctt agctgtcatc cagtccacta cgtcatcttg tgctagcgac
     1801 gaagctgccg cttccatggc aaagccgctg gtaaacatct tcggtagaac gatcacatcg
     1861 cgcccggtaa taccttccag ttgacgatca aaatgacgca ggttggcagg gccatccatc
     1921 cacaccagtg gttgctgcaa aagcgtaatc ttcaaaccag gcacggtgta caactccttt
     1981 atgcgaaggg ttttataact ttaacacctt atcaggcagt tgccttagcg cagaataaat
     2041 tgataacaaa tgctgatatt ggaaatatct gatttgcaaa ttatcgtgtt atcgccaggc
     2101 tttagaaggt taataacatg ggcaggataa gctcgggagg aatgatgttt aagacaataa
     2161 cgacagtcgc cgctctggtc atcgccacca gtgcaatggc gcaggatgat ttaaccatta
     2221 gcagccttgc aaagggcgaa accaccaaag ctgcatttaa tcagatggta caagggcata
     2281 agctgcctgc ctgggtgatg aaaggcggta cttatactcc cgcacaaacc gtaacgttgg
     2341 gagatgatac gtatcaggtg atgagcgcgt gcaaaccgca tgactgtggc tcgcaacgta
     2401 tcgctgtgat gtggtccgag aaatctaatc agatgacggg gctgttctcg actattgatg
     2461 agaaaacgtc gcaagagaaa ctcacctggc tgaatgtgaa cgatgcgctt tcgattgatg
     2521 gtaaaacggt gctgttcgcg gcgttgaccg gcagcctgga aaaccatccg gatggcttta
     2581 attttaaata attagcggat aaagaaacgg agcctttcgg ctccgttatt caattacgcg
     2641 gcttcaactt tccgcacttt ctccggcaac tttaccggct tcgtcgccag ctcttccgga
     2701 tcaaagtcat caacgttaat actgcgcaga cggctttctt cagctttcac cagaatagcg
     2761 gcttcatctt tatcaatcag ccccttcgcc agcgcgttgt gcgccagttc atccagacgg
     2821 gtaaacggca ggtttttacc cagctcttta cagatccgct gatgaattgg gtcggcggca
     2881 atcacatcca ccagcgcctc ttccagcaag ccaaccggat tatgctcgct cggcgtcagg
     2941 tactgaccgc gaccaatgcg ggaacgggtg gcgttcggca cttgtaaaat cttcgccact
     3001 ttatgatcca gcttgtcaga aggtgccaga taatgacgtc cggtcgggaa gatcaccaca
     3061 ttcagcagcc cggcaaccac gcggttcggg aagttttgca gtaaatcatc catcgcctgt
     3121 tcagcctgat acagcgcatc ttgtacgccc cagtgcacca gcggcaggtc ggcttcatta
     3181 cggccttcgt cgtcataacg cttcagcacg gcagaggcga ggtagagctg gcttaaaata
     3241 tcccccagac gggccgagat gcgctcgcga cgtttcaggc tgccgtccag cactgccatc
     3301 gagacatcag aaagcagggc gaggttggcg ctcaggcggt tcaggtgctg atagtagcgt
     3361 ttagtggcat cgccggttgg cgtgctgctg gttaaaccgc gcgtcaggcc cagccagaag
     3421 ctgcgaactt tgttgctacc gacgtgaccg atatgtttga acaacagttt atcgaacgcg
     3481 ttgacgtcat tgttcttcgc cgcttccatc tcttccagca cgtacggatg gcaacgaatc
     3541 gctccttgtc cgaagatcat catgctgcgg gtcagaatgt tagccccttc aacggtgatg
     3601 gcaatcggtg cgccctggta agcacgcgcc aggaagttgc tttgcccgag cataatgcct
     3661 ttgccaccag tgatatccat cgcatcaata atcgactgct gcccgcggtg ggtacagtga
     3721 tacttaacga tagccgacag cacggcaggt ttttcgccga gcataatgcc gtaggtaatc
     3781 agcgatgccg cagcatccat cacgtaggca ttaccggcaa tacgcgccag cggctcttca
     3841 atcccttcca tcttaccgat agagattttg aactgacggc gaatgtgagc atacgcgccc
     3901 gttgccagcg ctaccgattt cacgccgccg gttgagttgg aaggcagggt gatgccgcgg
     3961 cctaccgaga ggcactccac cagcatccgc cagccttgcc cggccatttt cggcccaccg
     4021 atgatgtaat cgatcggtac gaagacatct ttaccgcgcg tcggtccgtt ctggaacggt
     4081 acgttcagcg ggaagtggcg acgaccaatt tccacgcccg gcgtggtggt cgggatcagc
     4141 gcacaggtaa tacctaaatc ttctgcgcca cccagcagct tttctgggtc ggagagttta
     4201 aacgccagcc caagcacagt cgcaatcggt gccagcgtaa tatagcgttt gttccaggtc
     4261 agacgcatcc ccagcacctg ctggccctgc cattcgccca tgcagacaat cccggtgtcc
     4321 ggaatcgcgc ccgcatcgga acccgcttcc gggcttgtca gtgcaaagca agggatctcc
     4381 tgaccacgcg ccagacgcgg cagatagtga tctttctgct cgtcagtgcc gtaatgttgc
     4441 agcagctcgc ccgggcctaa tgagttcggc acgccgacgg taatcgccag gatcccgctc
     4501 acgccggaga gtttttgcag cacgcgagac tgggcataag ctgagaactc cagtccgccg
     4561 tactcttttt tgataatcat cgcgaagaag cgatgctctt taaggtacgc ccacaactcc
     4621 ggcggcagat ccgccagctc atgagtaatc tggaaatcat tcgccatccg gcaggcttct
     4681 tctaccgggc cgtcgagaaa cgcttgctct tcggcggtca ggcgcggctg cggatagtta
     4741 tgcagctttt tccagtccgg cttgccctgg aacaagtcgc cctcccacca ggtggtgccc
     4801 gcatcaatcg cttctttctc agtgcgcgac atcggcggca tcaccttacg gaaaccgcga
     4861 aataccggcg cggaaatcat cgacttacgc ataggcgcaa agttaaatgg cacgaggata
     4921 atggccagag gcaccagtac ccacgctgac cacagaccag caacgccgag ggcggctgtc
     4981 caggcgagca aaatcagact gctgataaat aagctcacgc ggtgatagaa caacgcgccg
     5041 agcaggacaa ccgtagcgag aatactcaaa atcatcataa cgaaaagccc cttacttgta
     5101 ggaggtctga ccacttgtga tgatatggtt gtagtggatg taaaaacatt tagcaatgtg
     5161 tttacaatat aattacaaca aagctcacat tgttgctgtt tttatccgca cttcaggtca
     5221 aaaagtcctg gtcatagcac ctgcccgtac ttctcgcttt tggcggtatc cggtacactg
     5281 cattttgtct attacattta tgctgaagga tatcctcatg taccaggatc ttattcgtaa
     5341 cgaactgaac gaagcggcgg aaacgctggc taacttttta aaagatgacg ccaatattca
     5401 cgccattcag cgcgcggcgg tcctgttagc agacagcttt aaagccggtg gcaaagtgct
     5461 ttcctgcggc aacggcggtt cccattgcga cgctatgcac tttgccgaag agttgaccgg
     5521 tcgctaccgt gaaaaccgtc cgggctaccc ggcgattgct atttctgacg ttagtcatat
     5581 ttcctgcgtc ggtaatgatt tcggtttcaa tgatattttc tcccgctacg ttgaagcggt
     5641 aggtcgcgaa ggcgatgtac tgctggggat ctccacctcc ggtaactctg caaacgtgat
     5701 caaagcgatc gcagcggcgc gtgagaaggg gatgaaagtg atcaccctga ccggtaaaga
     5761 cggcggtaaa atggctggca cggcggatat cgaaattcgc gttccgcact ttggttatgc
     5821 cgaccgcatt caggagattc acattaaagt gatccatatc ctgatccagt taattgaaaa
     5881 agagatggtt aagtaagtct ggcgtaggcc ggataaggcg tttacgccgc atccggcact
     5941 tgtgcgctga tgcctgatgc gacgctgatg cgtcttatca tgcctacaaa tctgtacgcg
     6001 aaccgtaggc cgaataagac gtttacgccg catccggcct gaaaattctt aaatcaatct
     6061 tcgccggggg ccatgcgctc ccgctgttgt ggaggttacc catgtgcgaa ttgctcggga
     6121 tgagcgccaa cgtccctacc gatatctgct ttagtttcac cggtctggtg cagcgtggtg
     6181 gtggaaccgg gccacataaa gatggctggg gcattacctt ttacgaaggt aaaggctgtc
     6241 gcacatttaa agatccacaa cccagcttta attcccccat cgccaaactt gtccaggact
     6301 acccgataaa atcctgttcg gtggtggctc atattcgcca ggccaatcgg ggcgaggtgg
     6361 cgctggaaaa tactcacccg tttacccgtg agttatgggg gcgtaactgg acttatgccc
     6421 ataacggaca actgacgggc tacaaatcac tggaaaccgg caacttccgc ccggtcggtg
     6481 aaaccgatag cgaaaaagcc ttctgctggc tcctgcataa attaacgcag cgttacccgc
     6541 gcacaccggg caacatggcg gcagtgttta aatatatcgc ctcactggcg gatgaactgc
     6601 ggcagaaggg cgttttcaac atgctgcttt ctgacgggcg ctatgtgatg gcgtattgct
     6661 cgactaattt acactggatc acccgccgcg cgccgtttgg cgtggcaacg ctgctggatc
     6721 aggatgtgga aatcgacttc agctcgcaga ccacaccgaa tgatgtggtc acggtgattg
     6781 caacacagcc gctgacgggc aatgaaacct ggcaaaagat tatgccaggc gaatggcgct
     6841 tattttgcct cggggagcgt gtagtttgat gccagttgtg gctgcacaac ttcgtggctt
     6901 aacggcttgc tgaccacgta acgaccattg accacagaaa cggtcggtgg cttacgggtt
     6961 tgctcaaagt agtcgtaacc cggcttcagt tgctcccaga agtccttaaa gttggaatat
     7021 ttatggcgct tcatattggc gtcggtcatg cggaacgggt aaatactcac ttgcacgctc
     7081 ggctgaccaa acaccagcgc accagtaacg aactggaaga tctcatcaat accctgattg
     7141 gtcattgcgt agcagccgat ggaaacacaa tcgccgtgga tcatcaggta tttcccttca
     7201 taaccatgcg cacggtcata ggcattgggg aaaccaatat taatcgcttt gtagtaacgg
     7261 ctgtctggtt ttaactgatt acgctggacg ctataaaacc cttccgggct tttgaaatcg
     7321 ccctgacgct gttttggccc taagccgccg gaatatttac agattttata actgtcgagc
     7381 agctgatatt gctcgcccat tttgacgtag agatcgagcg tacgttcttc cttgaagatc
     7441 tggatgtaga caggggatcc catcaactgc tgcttatact ctttgctcac tggcgtggtc
     7501 gagctactgc tgcccagcaa accggcaaac gaaacgcacg ggatcaacaa catcgcaaga
     7561 attaatgcga ttttacgcat actgcttatt ccttgataaa acggttacac acgccaggac
     7621 ggcaaaatag atcccaaatc ggaatagtct ggatttggaa ggctcacatt atcaccaaaa
     7681 gagttttacg caagcctgtc ggcgcagggt ttacaaattt catcggcagc gtgctttacc
     7741 caaagagcgc cgcgtgagtt ccagttctct caaatcgtaa aagtttatcg gtaagtttgt
     7801 aaatcaggat ccagtccggt tcgacatgag catcgcgata acctttccat gaaccttgca
     7861 gcgggtggtc tttataaaca gctggaagcg gtaaagcatt attgataaga agcgtcataa
     7921 gatatttcaa tttattcata tccttatgac gcttttgtgc aagttttaca tcctttgaaa
     7981 attgtcccga gtattcaata tccctttgaa tcatatttaa attcctaatt tatcaaataa
     8041 atcatcagcg tctttggcct tatgaacatc aacgccagct tcgctgtttt tgattgattg
     8101 aatggttaat tgattaggct cgcgtaaatc aaacggcaat gccttttcac gcgcgacctt
     8161 tgtgagggtt atgcgaacca ggtcagagat ggtcagcccc atcccggcca gtacgtccgc
     8221 cgcctgattc ttcagatctt catcgattcg ggcgcgaaca aacgcgttag cagccattgg
     8281 gggatctcct gtgctgtata tttattcagc ttgaattgta gctcaaatga gcaacagcga
     8341 caatccatga gtaatt
//
LOCUS       DAZPLI010000167         8325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZPLI010000167 DAZPLI010000000
VERSION     DAZPLI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8325
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.168"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..706
                     /locus_tag="ACHUU3_003610"
     CDS             <1..706
                     /locus_tag="ACHUU3_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR2083588.1"
                     /translation="WVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNKD
                     ATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHDD
                     NSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKRL
                     ADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKILT
                     DTSKLIYDLFGVKSEK"
     gene            1069..2106
                     /gene="dusA"
                     /locus_tag="ACHUU3_003611"
     CDS             1069..2106
                     /gene="dusA"
                     /locus_tag="ACHUU3_003611"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR2083589.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2240..2482
                     /gene="pspG"
                     /locus_tag="ACHUU3_003612"
     CDS             2240..2482
                     /gene="pspG"
                     /locus_tag="ACHUU3_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR2083590.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2648..3631)
                     /locus_tag="ACHUU3_003613"
     CDS             complement(2648..3631)
                     /locus_tag="ACHUU3_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR2083591.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3714..5129
                     /gene="dnaB"
                     /locus_tag="ACHUU3_003614"
     CDS             3714..5129
                     /gene="dnaB"
                     /locus_tag="ACHUU3_003614"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR2083592.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5182..6261
                     /gene="alr"
                     /locus_tag="ACHUU3_003615"
     CDS             5182..6261
                     /gene="alr"
                     /locus_tag="ACHUU3_003615"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR2083593.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6514..7707
                     /gene="tyrB"
                     /locus_tag="ACHUU3_003616"
     CDS             6514..7707
                     /gene="tyrB"
                     /locus_tag="ACHUU3_003616"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR2083594.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(8211..8325)
                     /locus_tag="ACHUU3_003617"
                     /pseudo
     CDS             complement(8211..8325)
                     /locus_tag="ACHUU3_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2060 a         1951 c         2108 g         2206 t
ORIGIN      
        1 gtgggtcaat aagtatattg acgattgcac tgatgaggat ttaaacgatc gtgactttat
       61 tgcatcagtt gttgaccggg ctatttttca tttcgcgatt aatagtatat gtaatcctgg
      121 ggataataaa gatgcgactc ccattgaaca atgtactttt gatgtagaaa ctaagaatgg
      181 ccttccctcc acggttcagc tattttatga ggaatctaag gataatgaac ctttagcgaa
      241 tatacatttt caagcaatag gttctggttt tttaacgttt gttaatgcct gccaggaaca
      301 tgatgacaac agcttaaaat tatttgcttc gctgttaatt tcactttcat attctagtgc
      361 ctacgcagat ttatcagaaa cagtgtatat taatgaaaat aatgagagct acctgaaagc
      421 tcagtttgaa aaattatctc aacgtgatat gaagaagtac ctgggagaga tgaagcgtct
      481 ggctgatggg ggagaaatga attttgatgg ctatctggat aagatgtcac atctggtgaa
      541 tgaaggaacg ctcgatcctg atattttaag caaaatgcga gatgctgcac cacaattaat
      601 tagcttcgcg aagtcgtttg acccaacctc aaaggaagag attaaaatac ttacagacac
      661 ttctaaatta atttatgatt tgttcggggt taaatcggag aaataatatg tgaagttctt
      721 cgatagtatg gaaggcatta tataaaagga cccaatattt attgggttct tttttctcta
      781 tcaatgctat tagcagggag atatatcacc agagtttaat gtgtgatttt ttatttatcg
      841 tcgaacctgg attgtttatc attggcctta acaaagttaa cggctaataa gattatttcc
      901 atcacttcgt gagagcttca tgccttgaga ggatctcaat tttcttttgc aatgagacag
      961 gcgcttcctg ttgttatggt atagtacccc gctattgagc ctcctgaata gtgatgctga
     1021 ataacataac cccatgatat atcgataaaa taatctctac atttgaaaat gcacggtaat
     1081 tctgaaatgc aaaaaatcaa ccaaaccagc gcaatgcctg aaaaaactga cgttcactgg
     1141 agtggtcggt ttagcgttgc accaatgctc gactggacgg acagacattg ccgctatttc
     1201 ttgcgtctgc tttcccgcaa tacgttgctg tataccgaaa tggtgaccac aggggcgatt
     1261 attcacggta aaggtgatta cctggcgtac agtgaagaag aacatccggt agcgttgcaa
     1321 ctcggcggta gcgatccggc ggcgctggca cagtgtgcga agctggcaga agcgcgtgga
     1381 tatgatgaga tcaacctgaa tgtcggctgc ccgtctgacc gggtgcagaa cggcatgttt
     1441 ggtgcgtgtc tgatgggtaa tgcgcagctg gttgccgact gcgtgaaagc gatgcgcgat
     1501 gtggtgtcga ttccagtgac ggtgaaaacg cgtattggca tcgatgacca ggacagctat
     1561 gaatttctct gcgatttcat caacaccgtt tccggcaaag gcgagtgtga gatgtttatc
     1621 atccacgcac gtaaagcctg gctttcgggg ttaagcccga aagaaaaccg tgaaatcccg
     1681 ccgctcgatt atccgcgtgt gtatcaactg aagcgtgact ttccgcatct gacaatgtcg
     1741 attaacggtg gtatcaagtc gctggaagag gccaaagcac acctgcaaca tatggatggc
     1801 gtgatggtcg ggcgcgaggc gtatcagaat ccgggtattc tggcggcggt agaccgggag
     1861 atctttggtt cctcggatac cgatgccgat ccggtggcgg tagtgcgcgc catgtatccg
     1921 tacattgagc gtgaactcag ccaggggacg tatctcggcc atattacccg gcatatgttg
     1981 ggcttgttcc agggtattcc tggcgcgcgg cagtggcggc gttatttaag tgaaaatgcc
     2041 cataaagcgg gtgcagacat taatgtgctg gaacacgcgc tcaaactggt ggcggataag
     2101 cgttaacttt tcaccaaaaa gtagtcaaat tcaccacgcc ctgcgcaccg tcgcggggcg
     2161 ttttgctgtt aaatcaatag attatttttg gcatgattct tgtaatgcca gcaagagatt
     2221 tcatatttgg gagagcatca tgctggaact actttttgtg attggctttt ttgtcatgct
     2281 gatggtcacc ggcgtttcgt tgctgggcat tatcgccgcg ctggttgtgg cgacggccat
     2341 tatgttcctc ggcggtatgc tggcattgat gattaagttg ctgccgtggt tactactggc
     2401 gattgcggtg gtgtgggtta ttaaggcgat taaagcacca aaagtgccga aatatcagcg
     2461 ttatgaccgc tggcgttact aagggattgt gcggatgatc acaacctaag gttttatcct
     2521 tagaacaaaa taggaattga taatcaaatc tgtcactatt gcgcctctaa cagattcatc
     2581 gtgctgtacc ctacatacag ccgaactata aaaagaaagg gcttcccagg tggaagccct
     2641 atttctttta tggaatcaac aggctggaac cttgcgtcgc ccggctttcc agaatctcat
     2701 gcgcacgctg cgcatccttc agcggatatt tctgctgctc ggcgacatcg accttaatca
     2761 caccgctggc aatcaaagag aacagttcat tactggcctc ggttaattcc tcccgcgtgg
     2821 tgatatagcc ttgcagggaa gggcgtgtca catacaacga gcctttttga ttgagaatgc
     2881 ctaagttcac accggtaacc gcacctgatg agttgccaaa actgaccatt aagccgcggc
     2941 gttgcaggca atccagcgac cgttcccagg tgtctctgcc cacggaatcg tacaccacgc
     3001 gcactttctt accgccggtg atctctttta accgctcgac cagatcctct tcacgatagt
     3061 taataacctg ccacgcgccc gcttttagcg cgctctgcgc tttttgcgcg gttcctacgg
     3121 tgccgataag tttcgcgccc agggcttttg cccactggca ggcaattaag ccaacgccgc
     3181 cagccgctgc gtggaacagg aactgttcat cgggtttaat ttcataggtt ttgcgcagca
     3241 gataataaac cgttaagcct ttcaggaagg atgccgcagc ttgctcaaaa gaaattgccg
     3301 caggcagaat cgccgcttta tccgcattaa tgttatgcac agagctgtaa gcgcctaacg
     3361 ccgactgcgc atagactaca cgatcgcctg ccttaatatg ctttacacca ctgccgactt
     3421 tactcacgat gcctgccgct tcggtaccta atccgctggg tagtgatggc ggcgggtaaa
     3481 ggccgctgcg gatatacgtg tcgataaaat tgatgccgat ggctttattt tcgacctgga
     3541 tttcattctc tgccggatcg gcaggagtga actctacggc ttgaagtact tccgggccac
     3601 cgtgcttgtg aaattcaatt cgtgttgcca tgtgtcctcc agaacgtatc gtcagggtct
     3661 gcttcatatg gtaaagtttc gacccattct ttatctcggt aactccattc actatggcag
     3721 gaaataaacc cttcaacaaa cagcaggctg aaccccgcga acgcgatcca caagttgccg
     3781 ggctgaaagt gcctccgcac tcgatcgaag cggagcagtc ggtgttgggc ggtttaatgc
     3841 tggataacga acgctgggac gatgtagccg agcgtgtggt ggcagacgat ttttacaccc
     3901 gcccacaccg ccatatcttt actgaaatgg cgcgtttgca ggaaagcggt agtcctatcg
     3961 atctgattac ccttgcggaa tcgctggaac gccaggggca actcgatagc gtcggtggtt
     4021 ttgcttatct ggcagagctg tcaaaaaata cgccaagtgc ggcgaacatc agtgcttatg
     4081 ctgacatcgt gcgtgaacgt gccgttgtcc gtgagatgat ctcggttgcg aatgagattg
     4141 ctgaagccgg ttttgatccg caggggcgta ccagcgaaga tctgctggac cttgctgaat
     4201 cccgcgtctt taaaattgcc gaaagtcgtg caaacaaaga cgaagggccg aagaacatcg
     4261 ccgatgtgct cgacgcaacc gtggcgcgta ttgagcagtt gtttcagcag ccacacgatg
     4321 gcgttaccgg agtaaacacc ggttatgacg atctcaacaa aaaaaccgct ggcttgcagc
     4381 cgtcggattt gatcatcgtc gccgcgcgtc cgtcgatggg taaaacaaca tttgcgatga
     4441 acctcgtcga aaacgcggcg atgttgcagg ataaaccagt acttatcttc tcgctggaga
     4501 tgccttcaga acagattatg atgcgttctc tggcgtcgct gtcgcgcgtt gaccagacta
     4561 aaatccgtac cgggcagctc gatgatgaag actgggcgcg catttccggc accatgggta
     4621 ttttgctcga aaaacgcaat atctatatcg atgactcctc cggcctgacg ccaacggaag
     4681 tgcgttcccg cgcacgccgt attgcccgtg aacacggcgg catcgggctt atcatgatcg
     4741 actacctgca actgatgcgc gtaccggcgc tttccgataa ccgtacgctg gaaattgcag
     4801 aaatctctcg ctcgctgaaa gcactggcga aagaactgaa cgtgccggtg gtggcgctgt
     4861 cccagttgaa ccgttctctg gaacaacgtg ccgacaaacg cccggtcaac tccgacctgc
     4921 gtgaatctgg ctctatcgag caggatgcgg acttgattat gtttatctat cgtgatgagg
     4981 tgtatcacga aaacagtgat ttaaaaggca tcgcggaaat tattatcggt aaacaacgta
     5041 acggcccaat cgggacggta cgcctgacct ttaacggtca atggtcgcgc ttcgacaact
     5101 atgcggggcc gcagtacgac gacgaataat aattatttta tgaattaggt aattaaagca
     5161 aacacttatc aaggaacaca aatgcaagcg gcaactgttg tgattaaccg ccgcgctctg
     5221 cgacacaacc tgcaacgtct tcgtgaactg gcccctgcca gtaaaatggt tgcggtggtg
     5281 aaagcgaacg cttatggtca cggtcttctt gagaccgcgc gaacgctccc cgatgctgac
     5341 gcctttggcg tagcccgtct cgaagaagct ctgcgactgc gtgcgggggg aatcaccaaa
     5401 cctgtactgt tactcgaagg cttttttgat gccagagatc tgccgacgat ttctgcgcaa
     5461 cattttcata ccgccgtgca taacgaagaa cagctggctg cgctggaaga ggctagcctg
     5521 gacgagccgg ttaccgtctg gatgaaactc gataccggta tgcaccgtct gggcgtaagg
     5581 ccggaacagg ctgaggcgtt ttatcatcgc ctgacacagt gcaaaaacgt tcgtcagccg
     5641 gtgaatatcg tcagccattt tgcgcgcgcg gatgaaccaa aatgtggcgc aaccgagaaa
     5701 caactcgcta tctttaatac cttttgcgaa ggcaaacctg gtcaacgttc cattgccgcg
     5761 tcgggtggca ttctgctgtg gccacaatcg cattttgact gggtgcgccc gggcatcatt
     5821 ctttatggcg tctcgccgct ggaagatcgc tccatcggtg ccgattttgg ctgtcagcca
     5881 gtgatgtcac taacctccag cctgattgcc gtgcgtgagc ataaagccgg agagcctgtt
     5941 ggttatggtg gaacctgggt aagcgaacgt gatacccgtc ttggcgtagt cgcgatgggc
     6001 tatggcgatg gttatccgcg cgccgctcca tccggtacgc cagtgctggt gaacggtcgc
     6061 gaagtaccga ttgtcggacg cgtggcgatg gatatgatct gcgtagactt aggtccacag
     6121 gcgcaggaca aagccgggga tccggtcatt ttatggggcg aaggtttgcc cgtagaacgt
     6181 atcgctgaaa tgacaaaagt gagcgcttac gaacttatta cgcgcctgac ttcaagggtc
     6241 gcgatgaaat acgtggatta atcgttctgt aatatttgat tgtctgtgcc ggatgcggcg
     6301 tgaatgcctt atccggccaa taaaatccta aaaattcaat aagttgatgt tctttcatgc
     6361 tcttataaag gtcgtgcctc tggcggatgt tcgtttgtca tgagtctcac tctgttgcta
     6421 attgccgttc gctcctgaac atccactcga tcttcgcctt cttccggttt attgtgtttt
     6481 aaccacctgc ccgtaaacct ggagaaccat cgcgtgtttc aaaaagttga cgcctacgct
     6541 ggcgacccga ttcttacgct tatggagcgt tttaaagaag accctcgcag cgacaaagtg
     6601 aatttaagta tcggtctgta ctacaacgaa gacggaatta ttccacaact gaaagccgtg
     6661 gcggaggcgg aagcgcgcct gaatgcgcag ccccatggcg cttcgcttta tttaccgatg
     6721 gaagggctta acagctatcg ccatgccatt gcgccgctgc tgtttggtgc ggaccatccg
     6781 gtactgcaac aacagcgcgt agcaaccatt caaacccttg gcggctcagg ggcattgaaa
     6841 gtgggcgcgg atttcctgaa acgctacttc ccggaatcag gcgtctgggt cagcgatcct
     6901 acctgggaaa accacgtagc aatattcgcc ggggctggat tcgaagtgag tacttacccc
     6961 tggtatgacg aagcgactaa cagcgtgcgc tttaatgacc tgttggcgac gctgaaaaca
     7021 ttacctgccc gcagtattgt gttgctgcat ccatgttgcc acaacccaac gggtgccgat
     7081 ctcactaatg atcagtggga tgcggtgatt gaaattctca aagcccgcga gcttatccca
     7141 ttcctcgata ttgcctatca aggatttagt gccggtatgg aagaggatgc ctacgctatt
     7201 cgcgccattg ccagcgctgg attacccgct ctggtgagca attcgttctc gaaaattttc
     7261 tccctttacg gcgagcgcgt cggcggactt tctgttctgt gtgaagatgc cgaagccgct
     7321 ggccgcgtac tggggcaatt gaaagcaaca gttcgccgca actactccag cccgccgaat
     7381 tttggtgcgc aggtggtggc tgcagtgctg aatgacgagg cattgaaagc cagctggctg
     7441 gcggaagtag aagagatgcg tactcgcatt ctggcaatgc gtcaggaatt ggtgaaggta
     7501 ttaagcacag agatgccaga acgcaatttc gattatctgc ttaatcagcg cggcatgttc
     7561 agttataccg gtttaagtgc cgctcaggtt gaccgactac gtgaagaatt tggtgtctat
     7621 ctcatcgcca gcggtcgcat gtgtgtcgcc gggttaaata cggcaaatgt acaacgtgtg
     7681 gcaaaggcgt ttgctgcggt gatgtaatgc aggaaagcag gctggagtta cccagcctgc
     7741 agtgaaatta aactgtcgtc gctttcacta tttctttgta gatgattttt ttgatgccgt
     7801 tgttctacgt gagagataat aaaacgttgt cagttctttt attgttaagt ttatcccaat
     7861 tatctggaat tcctttccct attttttcgt gtggagtaat aatttctgca catctaacca
     7921 ttattaaatt taataataag tatcgggttt gttacgatag aatatttcta tatcctcaag
     7981 aatatacttt gccttattgc ctgtgacttt ctgagattca attatagtat cgttaaattc
     8041 taatgttaaa gagaactctt tttttccgct ttgtgtaagt ttaaccttat attgattacc
     8101 aatatcaaat aaaaaggtcc tgtaatgaga ataatcacct ttaacctcct cggcaaaaca
     8161 gcaaagcgtg caaatataca taaagtcttt cttacccctg attttgttaa ctaaatcgat
     8221 aaatgcgtga aaaactttac ttgcaataga acttgatact tcttctccag gtctacaaaa
     8281 atcttttgca atttttaact gtgtttctat gtataaaaag aaatc
//
LOCUS       DAZPLI010000168         8304 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZPLI010000168 DAZPLI010000000
VERSION     DAZPLI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8304
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.169"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..196
                     /locus_tag="ACHUU3_003618"
     CDS             <1..196
                     /locus_tag="ACHUU3_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083595.1"
                     /translation="TIDLNIEDSSSEEFLFDNSLEKDENSSDNCFEVEKYNAYEAYNS
                     ENLYFTREEYTYDYDLLNAI"
     gene            complement(810..1186)
                     /gene="rnpB"
                     /locus_tag="ACHUU3_003619"
     ncRNA           complement(810..1186)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHUU3_003619"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1219..2364)
                     /gene="garK"
                     /locus_tag="ACHUU3_003620"
     CDS             complement(1219..2364)
                     /gene="garK"
                     /locus_tag="ACHUU3_003620"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR2083596.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2461..3351)
                     /gene="garR"
                     /locus_tag="ACHUU3_003621"
     CDS             complement(2461..3351)
                     /gene="garR"
                     /locus_tag="ACHUU3_003621"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR2083597.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3381..4151)
                     /gene="garL"
                     /locus_tag="ACHUU3_003622"
     CDS             complement(3381..4151)
                     /gene="garL"
                     /locus_tag="ACHUU3_003622"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFR2083598.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(4167..5501)
                     /gene="garP"
                     /locus_tag="ACHUU3_003623"
     CDS             complement(4167..5501)
                     /gene="garP"
                     /locus_tag="ACHUU3_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR2083599.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5876..7447
                     /gene="garD"
                     /locus_tag="ACHUU3_003624"
     CDS             5876..7447
                     /gene="garD"
                     /locus_tag="ACHUU3_003624"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR2083600.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7596..7973
                     /locus_tag="ACHUU3_003625"
     CDS             7596..7973
                     /locus_tag="ACHUU3_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
                     /protein_id="HFR2083601.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNNPQKTRPFNIQHSTFNIQHSTFN
                     IQQGKKLVAGMDVNIDDEIGDDE"
     gene            7973..>8304
                     /gene="yhaV"
                     /locus_tag="ACHUU3_003626"
     CDS             7973..>8304
                     /gene="yhaV"
                     /locus_tag="ACHUU3_003626"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR2083602.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT         2131 a         2196 c         1992 g         1985 t
ORIGIN      
        1 cactatagat ttaaacatag aagactctag ctcagaggaa ttcctttttg ataatagcct
       61 cgagaaagac gaaaattctt cggacaattg ctttgaggtc gaaaaatata acgcatatga
      121 agcatataac tcagagaatc tatattttac cagagaggaa tatacgtatg attacgacct
      181 tttaaatgca atatagacat aataacttct tacaaaatct ataattaata ccgtagatat
      241 tcattcatct taccctgtgc accaaaagca aaaactcact gacgcgggtt tgaaactgat
      301 ggcaaccgca aaaatgcctg atgcgctacg cttatcaggc ctacgccatc tctacaatat
      361 attgaatttg cgtgcttttg taggcaggat aaggcgttca cgcagcatcc ggcatgaata
      421 aagcgcactc aacaatctga aacccgccgg aacggtttat tacgtacatc aggtgaaact
      481 gaccgataag ccgctttctt ttgggtatag tgtcgtggac agtcattcat ctttctgccc
      541 ctccaaaagc aaaaacccgc cgaagcgggt ttttacgtaa atcaggtgaa actgaccgat
      601 aagccgcttt cttttgggta tagcgtcgtg gacagtcatt catctttctg cccctccaaa
      661 agcaaaaacc cgccgaagcg gttttttacg taaaccaggt gaaactgacc gataagccgc
      721 tttcttttgg gtatagcgtc gtggacagtc attcatcttt ccgctcctcc aaaagcaaaa
      781 acccgccgaa gcgggttttt acgtaaatca ggtgaaactg accgataagc cgggttctgt
      841 cgtggacagt cattcatcta ggccagcaat cgctcactgg ctcaagcagc ctacccgggt
      901 tcagtacggg ccgtacctta tgaaccccta tttggccttg ctccgggtgg agtttaccgt
      961 gccacggact gttaccagcc gcgcggtgcg ctcttaccgc accctttcac ccttacctga
     1021 tcccgcttgc gcgggccatc ggcggtttgc tctctgttgc actggtcgtg ggtttccccc
     1081 ccaggcgtta cctggcaccc tgccctatgg agcccggact ttcctcccct ccgcccgtct
     1141 cccccgaaga ggacgacgac gaagcggcga ctgtctggtc agcttcggcg cgcagtatag
     1201 agggtttgcg cgcccttgtc accccgcgtt gcgcattcca atcgccagtg tcgcggcgat
     1261 attacgtgaa gcacggcaga tattgtcata agccccgcgg aatgcttcgt ccaacgtacc
     1321 tatgctggtc aatacgctga agaccgcatc gatgccatgc tgatgtacaa cgccaacatc
     1381 atcggtcagg ctacccgcaa tgccaatcac cggtttatgg tacttcttcg ccacgtttgc
     1441 aacgccaatc ggtaccttgc cgtgaatact ctggctgtca atacgccctt caccggtgat
     1501 caccagcgta caatcgtgaa tatgttcttc cagattcagc gccgtagtga cgatttcaat
     1561 accacttttc agttccgcac caagaaacgc catcagcgcc gcgcccatac cgcccgcagc
     1621 tcctgcgccg gggacatctt tcacatcaac atgcagcgct tttttaatga catcggcata
     1681 gtgagagagg ttattgtcca gctcaacaat catcgcttca ctggctccct tttgtgggcc
     1741 aaagatgcgc gatgcgccgt tatcgcccac cagcggattg gtgacatcac aagcgacgcg
     1801 aatgacgcaa tcttttaagc gcggatcgag gccggaaata tcaatatcat tcagagtatt
     1861 aagactaccg ccgccaaaac caatttcatt gccgttggcg tcgcataatt tcgcccccag
     1921 cgcctgtacc atgcctgcgc cgccatcatt tgtagcgctg ccgccaatgc cgataataat
     1981 gtttgttgca ctgctctcca gcgcctgcag gattaactcg cctgtgccgc gtgaagtggt
     2041 tacgagtgga tcgcgttttt ccgcaggtac cagctccagc ccactggccg ccgccatttc
     2101 aataaacgcg gttttgccat cgccggagat cccccaactg gcattcactt tctcgcccag
     2161 cggccctgta acccaggcgt gacgttcagc cccctgggtg gctgcaatca tcgcttccac
     2221 cgttccttca ccaccgtcgg caaccggaac agaaacatac tgtgcatcag gaaaaatttc
     2281 ccgaaatcct ttttctatcg cctgcgcaac ctcgctggca gataaacttt ctttataaga
     2341 gtctggggcg attacgattt tcatacctat gcctgttacc acatgacgcc ggagggcgtt
     2401 tctcttattc ggcctagatt ccaggcccgg attgcaatac accatccggg cacgacgtca
     2461 ttaacgagta acttcgactt tcgccagttt ttcgtagtag cacgccaggg cgctatgatc
     2521 cgccgttcct aaaccatctg cacgcagtgc ctgcatcatc tccataaccg cagctgtgag
     2581 cagcagttgt gcgccgacgc cgtgagaagt atccagcgca ttcgccagat ccttaatatg
     2641 cagatcaatg cggaagcccg gcttgaagtt gcggtccatc accatcggcg ctttggcatc
     2701 cagcacggta ctgcccgcca gcccaccgcg aattgcctga taaaccaggt ccgggttaac
     2761 gcccgctttg gttgccagcg ttaacgcttc tgacatcgca gcaatattca gcgccacaat
     2821 gacctgattt gccagtttgg tgacgttacc tgcaccgatt tccccggtat gcaccacgga
     2881 acccgccatc gctttcatca aatcatagta tttgtcgaaa atagccttgt cgccgcccac
     2941 catcactgac agcgtaccgt cgatggcttt cggttcaccg ccgctcaccg gagcatccag
     3001 catatcaatg cctttcgctt tcagcgcttc gctgatttca cggcttgcca gcggtgcgat
     3061 agaactcata tcgatcaata ccgtacctgg cttcgcgcct tcaataatgc cattctcacc
     3121 cagcgccacc tctttcacat gaggggagtt tggcagcatg gttatgatga cgtcgcactg
     3181 ttcagcgatc gctttagccg tagacgctgt ttctgcacct gcagcaatca cgtcagcaat
     3241 agcttctggg ttacggtcag caaccaccag cgagtaacct gctttcagaa ggtttttact
     3301 cattggttta cccataatcc ccaggccaat aaaaccaact ttcatagtca tatcaaccat
     3361 ctctcttgtt gcggtggtgg ttatttttta aaggtatcag ccagtttctg agtggcagag
     3421 cggaagacgc caagatcgct gccgacagcc acaaacgtcg cgccccattc cagataacga
     3481 cgcgcatcgg cttcgaccgg cgcgagaata ccgctgggtt tgccgtgcgc gctggcacgg
     3541 ttaaaaatat gctgaattgc tttttgtaca tccggatgcg aggcattgcc gagatggccc
     3601 aatgccgcag ccagatcgct ggggccgacg aagataccgt ctacaccttc ggtagcggca
     3661 atagcatcga cgttatctac gccctgctga ctttctatct ggaccagaat agtaatgttc
     3721 ttgttcgact gagcgaaata atccgccacg gtgccaaaca tattggcgcg gtgagaaacg
     3781 gagacgccgc gaatgccttc cggcgggtaa cgagttgatg ccaccgcctg ctctgcttcc
     3841 tcttttgttt ctacaaaagg aatcaggaag ttatagaaac cgatatccag aagacgctta
     3901 ataattaccg gctcgttggt cggcactcgc actactggcg cgctggcgct gcctttcagg
     3961 gccattaact gcggaataaa cgtggaaata tcgtttggcg catgttcgcc atccagcacc
     4021 agccagtcaa acccagccaa accaagaact tcagtgctaa tcgggttaga gagtgctgac
     4081 cagcaaccaa tttgtacctg tttcgcagcc agtgcggctt tgaatttatt cgggaaaaca
     4141 tcgttattca tcgcttatac ctttgcttat ttctgcaatt ccatacgttt aatgtcgcca
     4201 actacgaaga ggtagcagac catcgccatc agcgctgaac atcccacgaa aaccagtgct
     4261 gcattgaagg agtgcagttc acttaccagg tagccaatca ccagtggagt gacaatggag
     4321 gcaacattgc caaagacgtt aaagacgccg ccacagaggc caacaatctc tttcggcgcg
     4381 gtgtcagaaa tcaccggcca gcccagagca ccaaatcctt tgccaaaaaa agccagcgcc
     4441 atcagcatga ccaccagcgt ggtgttgttg gtgtagttac ataagatgat ggtggaaacc
     4501 agcaacattc ccagcacaat cggtagctta cgtgccaggg tcagggataa accgcgtttg
     4561 atcagataat ccgagaagac acctcccagc acgccgcccg caaaaccaca cagtgccgga
     4621 atcgaggcga ccagacccac tttcagaatc gacatgcctt tttcctgcac cagataaatc
     4681 gggaaccagg tgaggaagaa ccaggtgatg gtgttgataa aatattgtcc gaaaaatacg
     4741 cccaacatca tgcggttaga gagcaattgc ttgatgtaat gcagtttggg accgcttgct
     4801 gccgcgctgc ccggcttttt gtggtccata tcgaccaccg cgccattttc agagataaac
     4861 ttcagctctt ccgcagacat acgtgggtga tctgtcgggt tatgaatcaa cttgatccac
     4921 agcgccgtca gcacaaaacc aatccccccc ataacggtga agacgtgctc ccagccccaa
     4981 gcgaaagtca gccagccaag cagcggcgaa aagagcgcca gcgagaaata ttgcgccgag
     5041 ttaaagatgg cggaggcagt accacgttct ttcgtcggga accaggcggc gacaatacgg
     5101 gcgttcgccg ggaatgatgg cgcttccgag aaccccagca taaagcgcat aaagaacatg
     5161 gagatccctg cccaggccag cgggaacata tcaacaaagc cttgcaggaa ggtgaacagc
     5221 gaccagaaaa agaggctgta ggtgtaaact tttttcgagc caaacttatc aagcaaccag
     5281 ccgccgggga tttgcatcag caagtaggcc cagccaaaag cagagaagat gtaacccatc
     5341 gaaaccgcac ttaactgcaa ctcttttgcc acttcggtac cagcaataga cagcgttgca
     5401 cgatcggcgt agttaacggc ggtaacaata aaaataatca gtaatattaa atagctggta
     5461 tgcacgcctt tcttttttac gtcaacggtg tccagaatca ttttatttac ctcgggtact
     5521 tatgctgatt tttattatta tggggaaggt gttatttatg agttttattt atgccgttac
     5581 gacaatgaac tcgggaatta gtataagcag cgggagaata ataatcattg tgcaaatgct
     5641 aatttaatca gtactattta aatattattt tgagcatatg cacataaggt tgcgcgctaa
     5701 agcacagatt tgcgctttac cttaccgggc ggcactgcaa tccctgaaat gattgacatt
     5761 gatcacattt ctgcgtttaa actcctgaca ttcttatttc acccaatgaa gtcatttatt
     5821 tttaaatgag accagatcct cattttaata acccctggct ggagaatatt gcacaatggc
     5881 caacatcgaa atcagacaag aaacgccaac tgcgttttat ataaaagttc acgacacaga
     5941 taatgtggca attattgtta atgataatgg cctgaaagca ggaacgcgtt ttccggatgg
     6001 gctggaatta attgaacata ttccccaggg gcataaagtc gcattgctgg acattccagc
     6061 taatggtgaa attattcgtt atggcgaagt gattggttac gccgtgcgcg caatcccacg
     6121 cggaagctgg atcgacgaat caatggttgt actaccggaa gcgccgccgt tacacacgct
     6181 gccactggca accaaagtcc cggccccctt accgccgctg gaaggataca cctttgaggg
     6241 ctatcgcaat gccgatggca gcgtgggcac caaaaacctg ctcggcatca ccaccagcgt
     6301 ccactgtgtg gcaggcgtgg tggactatgt agtaaaaatc attgaacgcg atctgctacc
     6361 gaaatacccg aacgtcgatg gcgtggtggg gctgaatcat ttgtacggct gtggcgtggc
     6421 gattaacgca ccggcggcag ttgtgcctat tcgtaccatc cacaatattt cgctgaaccc
     6481 taactttggc ggcgaagtaa tggtgattgg tctgggttgt gaaaagttgc agcctgagcg
     6541 cctgctggtc ggcacggatg atgtgcaagc tattccagta gaaagcgcca gcattgtcag
     6601 tttgcaggat gaaaagcatg tcggctttca gtccatggtc gaggatattt tgcaggtcgc
     6661 cgaacgccat ctacaaaaac tgaatcaacg gcagcgagaa acctgcccgg cttcagaact
     6721 ggtcgttggt gtgcagtgcg gtggcagcga tgcgttttct ggtgtaacgg caaacccggc
     6781 ggttggctat gcgtctgatc tactggtgcg ctgcggcgca acggtgatgt tttcagaagt
     6841 aacggaagtg cgtgacgcga tccatctgct gacaccacgc gcagtgaacg aagaggtcgg
     6901 caaacggctg ctggaggaga tggagtggta cgataactat ctcaatatgg gaaaaaccga
     6961 ccgcagcgcc aacccttcgc cgggcaacaa gaaaggcggt ctggcaaatg tggtggagaa
     7021 ggcactcggc tccattgcta aatcgggtaa aagtgcaatt gttgaagtgc tgtcgcccgg
     7081 tcaacgcccg actaaacgcg gattaattta cgccgcgacg ccagccagcg attttgtctg
     7141 tggcacgcaa caggtggctt cgggtatcac cgtacaagtg tttacgaccg gccgtggtac
     7201 gccgtacggc ctgatggcgg tacccgtaat taaaatggca acccgcaccg agctggcgaa
     7261 ccgctggttt gatttaatgg atattaacgc gggcactatc gctaccggcg aagagagcat
     7321 tgaagaggtg ggctggaagt tgttccactt tattctcgac gtcgccagcg ggaagaagaa
     7381 aaccttctcg gatcaatggg ggctgcataa ccagctggcg gtgtttaacc cggcaccggt
     7441 gacctgattt cttttggaac gtccctcgca aaacatggcc ttagtgccat gtttttattg
     7501 tttaaagccc ccacgtccat taataatgca tttgcattac cttagttcaa gcttataatt
     7561 tgagcagaaa acaggctgta aaaggacaat gaatcatgcc cgctaatgct cgctctcacg
     7621 ctgtactgac cactgaatca aaggtcacga tacgcggaca aacaactatc cccgcgccag
     7681 tgcgtgaggc cttaaaactg aagccaggcc aggacagcat tcattacgaa attctgcctg
     7741 gtgggcaagt atttatgtgc cgactgggag atgaacagga ggatcatact atgaatgcat
     7801 ttttgcgttt tctggataca gatatccaga acaacccgca aaaaactcgt ccattcaaca
     7861 ttcaacattc aacattcaac attcaacatt caacattcaa cattcaacaa ggaaagaaac
     7921 ttgtcgctgg catggacgtc aacattgatg atgagattgg cgacgacgaa taatggattt
     7981 tccacaaagg gttaatggtt gggcgctata tgctcatccc tgttttcagg aaacctacga
     8041 cgctttagtt gccgaagtcg agacattaaa gggaaaagat cctgaaaatt atcagagaaa
     8101 agccgccaca aagttattgg cggtagtcca taaagtgatt gaggagcata tcacggtcaa
     8161 tccatcatca ccggcattcc gtcatggcaa gtcgttaggc tctgggaaaa ataaagactg
     8221 gtcacgggta aaatttggtg caggtcgtta tcgtctcttc tttcgttata gtgaaaaaga
     8281 gaaagtcatc attctgggat ggat
//
LOCUS       DAZPLI010000169         8298 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZPLI010000169 DAZPLI010000000
VERSION     DAZPLI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.170"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(42..110)
                     /gene="ykiE"
                     /locus_tag="ACHUU3_003627"
     CDS             complement(42..110)
                     /gene="ykiE"
                     /locus_tag="ACHUU3_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFR2083603.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            247..768
                     /gene="fecI"
                     /locus_tag="ACHUU3_003628"
     CDS             247..768
                     /gene="fecI"
                     /locus_tag="ACHUU3_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFR2083604.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            765..1718
                     /gene="fecR"
                     /locus_tag="ACHUU3_003629"
     CDS             765..1718
                     /gene="fecR"
                     /locus_tag="ACHUU3_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFR2083605.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1805..4129
                     /gene="fecA"
                     /locus_tag="ACHUU3_003630"
     CDS             1805..4129
                     /gene="fecA"
                     /locus_tag="ACHUU3_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFR2083606.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            4174..5076
                     /gene="fecB"
                     /locus_tag="ACHUU3_003631"
     CDS             4174..5076
                     /gene="fecB"
                     /locus_tag="ACHUU3_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFR2083607.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5073..6071
                     /gene="fecC"
                     /locus_tag="ACHUU3_003632"
     CDS             5073..6071
                     /gene="fecC"
                     /locus_tag="ACHUU3_003632"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFR2083608.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6068..7024
                     /gene="fecD"
                     /locus_tag="ACHUU3_003633"
     CDS             6068..7024
                     /gene="fecD"
                     /locus_tag="ACHUU3_003633"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFR2083609.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7025..7792
                     /gene="fecE"
                     /locus_tag="ACHUU3_003634"
     CDS             7025..7792
                     /gene="fecE"
                     /locus_tag="ACHUU3_003634"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFR2083610.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
BASE COUNT         1802 a         2379 c         2316 g         1801 t
ORIGIN      
        1 cagcattacc gaaatcccta tcctttaggg cagtgaggat gtcaataata gcagaccaca
       61 tcatgagtca ttctgaccga cacatgccag aagcagaggc tccatcccat tttataccta
      121 ccttataaca cttagaaaaa caacatgtta aaaatgtcta ttggaaacaa ttttatttcc
      181 aattgtaatg ataactattc tcatattaat atggctacgt gataattaac ttttgatgca
      241 ctccgcatgt ctgaccgcgc cactaccaca gcttccttaa cattcgagtc gctttatggc
      301 acacatcacg gctggttgaa aagctggctg acgcgcaaac tccagtctgc ttttgatgca
      361 gatgacattg cccaggacac ttttttgcgg gtaatggtca gcgaaacgct ctcgacgatc
      421 cgcgatcctc gctccttcct ctgcactatc gccaaacgcg tgatggtgga cctgtttcgc
      481 cgaaacgcgc tggaaaaagc gtatctggag atgctggcgc ttatgccgga ggggggagcg
      541 ccttcacctg aggaacgcga aagccaactc gagaccctac aactcctcga cagcatgctg
      601 gacgggctaa acggcaaaac acgtgaagcg tttctgcttt cgcaactgga tggtctgaca
      661 tacagcgaga ttgcgcacaa actcggtgtt tccatcagct ccgtgaaaaa atacgtggcg
      721 aaagccgtcg agcactgcct gctgttccgt ctggagtatg ggttatgaat cctttgttaa
      781 ccgattcccg ccgtcaggcg ctgcgttcag cttcccactg gtatgccgtg ctaagcggcg
      841 agcgcgtcag cccacaacag gaagcgcgct ggcaacagtg gtatgaacag gatcaggata
      901 accagtgggc ctggcagcag gttgaaaacc tgcgcaacca gcttggcggt gtgcctggcg
      961 acgttgccag ccgggcgttg cacgataccc gcctcacccg ccgtcacgtg atgaaaggat
     1021 tactgctgtt gctcggcgct ggcggaggct ggcagctctg gcagtcggaa accggcgaag
     1081 gtctgcgggc agattaccgc accgccaaag gcacagtcag ccgtcagcaa ctggaagatg
     1141 gctccctgct cacgctgaat acccaaagcg cggcggatat gcgttttgat gcgcatcagc
     1201 gcaccgtccg gctctggtac ggtgaaatcg ccattactac cgcgaaagat gccctgcaac
     1261 gccccttccg tgtcctgacc cgtcagggcc agctcactgc tttagggaca gaatttaccg
     1321 tccgccagca ggataatttc acgcagcttg acgtgcagca gcacgccgtg gaagtgcttc
     1381 tcgccagtgc ccccgcgcaa aaacgcatcg tgaacgctgg tgaaagcctg cagttcagcg
     1441 cctctgagtt tggcgcagtg aaaccgctgg atgacgagag tacaagctgg acgaaggaca
     1501 tcctgagctt cagcgataaa ccgctgggtg aggtgatagc cacgctaacc cgttaccgca
     1561 acggcgtgct gcgctgcgat cccgccgttg ccgggctgcg cctgagcggg acgttcccgc
     1621 tgaaaaatac cgatgcgatc ctgaacgtta tcgcgcaaac gcttcccgtt aaaattcagt
     1681 ctattacgcg gtactggata aacatttcac cactgtaagg aaaataattc ttatttcgat
     1741 tgtccttttt acccttctcg ttcgactcat agctgaacac aacaaaaatg atgatgggga
     1801 aggtatgacg ccgttacgcg tttttcgtaa aacaacacct ttggttaacg ccattcgcct
     1861 gagcctgctg ccgctggccg gtctctcgtt ttccgctttt gctgcacagg ttaatatcgc
     1921 accgggatcg ctcgataaag cgctcaatca gtatgccgca cacagcggat ttaccctctc
     1981 ggttgacgcc agcctgacgc gcggcaagca gagcaacggc ctgcacggcg attacgacgt
     2041 cgagagcggc ctgcaacaac tgctggacgg cagcggactg caggtaaaac cgctgggaaa
     2101 taacagctgg acgctggagc ccgcgcccgc accaaaagaa gatgccctga ccgtggtcgg
     2161 cgactggctg ggtgatgcgc gtgaaaacga cgtatttgaa catgctggcg cgcgtgacgt
     2221 gatccgccgt gaggatttcg ccaaaaccgg cgcaaccacc atgcgtgagg tacttaaccg
     2281 catccctggc gtcagcgcgc cggaaaacaa cggcaccggc agccacgacc tggcgatgaa
     2341 ctttggcatc cggggcctga acccgcgcct cgccagccgc tcgaccgtcc tgatggacgg
     2401 catccccgtc cccttcgccc cttacggtca gccgcagctt tcactggctc ccgtttcgct
     2461 cggcaacatg gatgccattg acgtggtacg cggtggtggt gcggtgcgtt acggaccgca
     2521 gagcgtgggc ggcgtggtga actttgttac ccgtgccatt ccgcaggact ttggtatcga
     2581 ggcgggcgtg gaaggtcagc tcagcccaac ctcttcacaa aacaacccga aagagacgca
     2641 caacctgatg gtgggcggca cagcggacaa cggttttggc accgcgctgc tctactccgg
     2701 cacgcgcggc agtgactggc gcgagcacag cgccacccgc atcgacgacc tgatgctgaa
     2761 aagcaaatat gcgccggatg aggtgcacac cttcaacagc ctgctgcaat attacgacgg
     2821 tgaagccgac atgcccggtg gcctgtctcg cgcggattac gacgccgatc gctggcaatc
     2881 cacccgcccg tatgaccgct tctggggtcg tcgcaagctg gcgagcctgg gctaccagtt
     2941 ccagccagac agccagcata aattcaacat tcaggggttc tacacccaaa ccctgcgcag
     3001 cggctacctg gagcaaggca aacgcatcac cctctcgccg cgtaactact gggtgcgcgg
     3061 tattgagcca cgctacagcc agatctttat gatcggccct tccgcgcacg aagtgggcgt
     3121 gggctatcgc tatgtgaatg aatcaacgca tgaaatgcgt tactacaccg ccaccagcag
     3181 cgggcagttg ccgtccggct caagccctta cgaccgcgat acgcgttccg gcaccgaggc
     3241 gcacgcctgg tatctggatg acaaaatcga catcggcaac tggaccatca cgccgggtat
     3301 gcgtttcgaa catatcgagt cataccagaa caacgccatc acaggcacgc acgaagaagt
     3361 gagctataac gcaccgcttc cggcgttgaa cgtgctctat cacctgactg acagctggaa
     3421 tctttatgca aacactgaag gctcgttcgg caccgtacag tacagccaga ttggcaaggc
     3481 tgtgcaaagc ggcaatgtgg aaccggaaaa agcgcgaacc tgggaactcg gtacccgcta
     3541 cgacgacggc gcgctgacgg cggaaatggg gctgttcctg attaacttta acaatcagta
     3601 cgactccaac cagaccaacg acaccgtcac tgcacgtggc aaaacgcgcc ataccgggct
     3661 ggaaacgcag gcacgttacg atctgggtac gctaacgcca acgcttgata acgtttccat
     3721 ctacgccagc tatgcgtatg tgaacgcgga aatccgcgag aaaggcgaca cctacggcaa
     3781 tctggtacca ttctccccga aacataaagg cacgctgggc gtggactaca agccaggaaa
     3841 ctggatgttc aatctgaaca gcgatttcca gtccagccag tttgcggata acgccaatac
     3901 ggtgaaagag agcgccgacg gcagtaccgg ccgcattccc ggcttcatgc tctggggcgc
     3961 acgcgtggtg tatgactttg gcccgcagat ggcagatctg aacctggcat tcggtgtgaa
     4021 aaacatcttc gaccaggact acttcatccg ctcttatgac gacaacaaca aaggcatcta
     4081 tgcaggccag ccgcgcacgc tgtatatgca gggggcgttg aagttctgat gtgttaacgc
     4141 ccggcttgcc gggcttttag ctggaatgtg attatgttgg catttatccg ttttcttttt
     4201 gcaggcctgc tgctggtgat cagccacgcc tttgccgcca cggttcagga cgaacacggc
     4261 acgtttacac tcgaaaaaac gccacaacgg attgtggtgc tggaactctc gttcgccgat
     4321 gcgctggccg ccgtggacgt cagcccgatc ggtattgccg acgataacga tgcaaaacgc
     4381 atcctgcccg aagtgcgtgc gcacctgaaa ccgtggcagt ccgtcggaac gcgcgcgcag
     4441 ccgagcctgg aagccattgc cgctctgaaa ccagacctga tcattgccga cagcagtcgc
     4501 catgcggggg tttacatcgc cttgcagcaa atcgcgtcgg tactgctgct taagtcccgc
     4561 aacgaaacct acgctgaaaa tttgcaatct gcggctatca tcggcgaaat ggtgggtaaa
     4621 aagcgagaga tgcaggcacg tctggaacaa cataaagaga ggatggcgca gtgggccagc
     4681 cagcttccca aagggacacg cgtggccttt ggcacatcac gcgaacagca attcaacctg
     4741 catactcagg agacctggac cggcagcgtg ctggcctctc tggggctgaa cgttcccgct
     4801 gcgatggcgg gtgcgtccat gccgtccatc ggcctggagc aactgctggc ggtcaatcct
     4861 gcctggctgc tggttgccca ctatcgcgaa gagagcattg ttaaacgctg gcaacaagat
     4921 ccgctctggc agatgttaac cgccgcgcag aagcagcagg ttgcttcggt cgacagtaac
     4981 acctgggcgc ggatgcgcgg tatttttgct gcagagcgta ttgccgctga cacggtaaaa
     5041 atcttccacc atcagccgct taccgttgtg aaatgaccgc gataaaacac ccggtgctgc
     5101 tgtgggggct tcccgttgca gcacttatta ttattttctg gctgagtctg ttttgctact
     5161 cggccattcc tgtttccgga gcagatgcaa cccgcgccct gctgcctgga cacacgccaa
     5221 cgctaccaga agcgctggtg caaaaccttc gtttgccacg aagcctggtc gccgttctga
     5281 tcggcgcaag cctggcgctc gcgggcacgc tgctgcaaac cctgacccac aacccaatgg
     5341 cctctccttc actgctcggc attaacagcg gcgcggcgct ggctatggcg cttaccagcg
     5401 cgctgagtcc gacgccgatt gcaggctatt ctctgtcgtt catcgcggca tgcgggggcg
     5461 gcgtgagctg gctgctggtc atgaccgcag gaggcggatt tcgtcatacc catgacagaa
     5521 acaaactgat cctcgcgggt atcgcgctgt cggccttttg tatgggcctt acccgcatca
     5581 ccctgctgct ggccgaagat catgcttacg gcatctttta ctggctggct ggcggagtgt
     5641 cccacgcccg ctggcaggat gtctggcagc tcttgccggt ggtggtcact gcagtccctg
     5701 tcgtgttgct gctggcgaat caactgaacc tgctcaacct cagcgacagc accgcccata
     5761 cgctgggagt gaacctgacg aggctacgtt tggtcatcaa tatgttagtg ctgcttctgg
     5821 ttggtgcgtg cgtcagcgtg gcaggtccgg tggcgtttat cggtctgctg gtgccacatc
     5881 tggcgcgctt ctgggcaggc ttcgatcagc gcaacgtact gccagtgagc atgctgctgg
     5941 gggccacgct gatgctgctg gcagatgtac tcgcacgcgc gctggccttc cccggagatc
     6001 tgcccgcagg cgcagtgctg gcgctgattg gcagcccttg ctttgtctgg cttgtgagga
     6061 ggcgaggatg aaaattgcgc tggttatttt catcaccctt gccctggcgg gctgtgcgct
     6121 gttatcactc catatgggag tgatccccgt gccgtggcgc gcgctgctga ccgactggca
     6181 ggccggacgc gagcattatt atgtattgat ggagtaccga ctgccgcgct tgctgctggc
     6241 actgtttgtc ggtgcagccc tcgccgtggc gggcgtgctg atacagggga ttgtgcgcaa
     6301 ccctctggca tcaccggata ttctcggcgt taaccatgcc gccagcctgg cctctgtggg
     6361 ggctctactt cttatgccgt cactgcccgt gatggtgctg ccgctgctgg cctttgcggg
     6421 cggcatggcg gggttgatat tgctgaagat gctggcaaag acccaccagc cgatgaagct
     6481 ggcactcacc ggcgtggcgc tttctgcatg ctgggccagc ctgacggatt atctgatgct
     6541 ctcgcgcccg caggatgtga acaacgccct gctgtggctg accggcagct tatggggccg
     6601 tgactggagc tttgtgaaga ttgccatccc gctgatgatt ttatttctgc cgctgagcct
     6661 gagtttttgc cgcgatctcg acctccttgc actcggcgat gcgcgcgcca ccacgctcgg
     6721 tgtgtcggtg ccctataccc gattctgggc tttgttacta gctgtcgcca tgacatctac
     6781 cggcgtggcc gcctgcggcc cgattagctt tattggtctc gtggtgccgc atatgatgcg
     6841 tagcatcacc ggtggacgtc accgcagact gctgcctgtt tcggccctga caggtgcgtt
     6901 gctgttggtg gttgccgatc tgctggcgag aattattcat cccccactgg agctcccggt
     6961 tggcgtgctg accgccatta tcggtgcgcc gtggtttgtc tggttgcttg tgagaatgcg
     7021 ataaatgact ttacgaactg aaaatctgac ggtcaattac gggacagaca aggtacttaa
     7081 cgacgtttca ctctcactgc caacggggaa gatcaccgcc ctgatcggtc ctaacggttg
     7141 cgggaaatcg acgctgttaa actgtttttc gcggctttta atgccgcagt ctggcaccgt
     7201 atttctcggc gataatccca taaatatgct ctcatcgcgc cagttggccc gcaggctttc
     7261 gctgctgcct cagcaccatt taacgccaga ggggatcaca gtccaggagc tggtttcgta
     7321 tggtcgtaat ccctggctgt cactctgggg gcgtctctcc gctgaagaca atgcacgagt
     7381 taatgtcgcc atgaaccaga cccggatcaa tcatcttgcc gttcgtcggt taaccgagct
     7441 ttccggcggt cagcgccagc gcgcatttct ggcaatggtc ctggcccaga atacgcccgt
     7501 tgtattactt gatgagccaa ccacctatct tgatatcaat caccaggtgg acctgatgcg
     7561 gttgatgggc gaactccgga ctcaggggaa aacggtggtc gctgtgctgc acgaccttaa
     7621 tcaggctagc cggtactgcg atcaactggt ggtaatggca aacggacatg ttatggcgca
     7681 aggcacacca gaagaggtga tgaccccagg attgctgaga acagtattca gcgtggaagc
     7741 ggaaatacac cccgagccgg tatctggcag gccgatgtgc ctaatgaggt agattgcaca
     7801 ggccgtaaga accaaaccac gactgaatga aactggactg gcgccagcaa gcctgttcag
     7861 actggggctg aacttttccg gactctgaaa gattaccaat actcatcgtc catccgcttg
     7921 ctttaggctg acaggttcat aatcaacgca aaccagagct gtacaggctt gggcgcggct
     7981 ttcaaaccag tcgtgatcac ggcaatcaat tttgaactct gcttaacgga catttctgta
     8041 taacccttac ggcaacgaaa aacgcgaagt taaaatttta gaaacccaaa aacgtgacat
     8101 gactaagttt agatttcagg ggggggagat caaaaaattt cgctctgtgc cagagcggac
     8161 attcacggag ctggttcatt accaatgagg ttgggctttt gaggataaat caatgatcag
     8221 acgccaacgt aaatcaaaag caaccctgga acggaattgc taatccagtt tctgaccatc
     8281 gatttttcta aaaagtgt
//
LOCUS       DAZPLI010000170         8185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZPLI010000170 DAZPLI010000000
VERSION     DAZPLI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8185
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.171"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..496
                     /gene="radC"
                     /locus_tag="ACHUU3_003635"
     CDS             <1..496
                     /gene="radC"
                     /locus_tag="ACHUU3_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR2083611.1"
                     /translation="LTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            713..949
                     /gene="rpmB"
                     /locus_tag="ACHUU3_003636"
     CDS             713..949
                     /gene="rpmB"
                     /locus_tag="ACHUU3_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR2083612.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            970..1137
                     /gene="rpmG"
                     /locus_tag="ACHUU3_003637"
     CDS             970..1137
                     /gene="rpmG"
                     /locus_tag="ACHUU3_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR2083613.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1235..2044
                     /gene="mutM"
                     /locus_tag="ACHUU3_003638"
     CDS             1235..2044
                     /gene="mutM"
                     /locus_tag="ACHUU3_003638"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR2083614.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="ACHUU3_003639"
     CDS             complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="ACHUU3_003639"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR2083615.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="ACHUU3_003640"
     CDS             complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="ACHUU3_003640"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR2083616.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4260..5318
                     /gene="rfaQ"
                     /locus_tag="ACHUU3_003641"
     CDS             4260..5318
                     /gene="rfaQ"
                     /locus_tag="ACHUU3_003641"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR2083617.1"
                     /translation="MDKPFLRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5315..6439
                     /locus_tag="ACHUU3_003642"
     CDS             5315..6439
                     /locus_tag="ACHUU3_003642"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR2083618.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PANQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6432..7229
                     /gene="rfaP"
                     /locus_tag="ACHUU3_003643"
     CDS             6432..7229
                     /gene="rfaP"
                     /locus_tag="ACHUU3_003643"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR2083619.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7245..>8185
                     /gene="waaO"
                     /locus_tag="ACHUU3_003644"
                     /gene_synonym="rfaI"
     CDS             7245..>8185
                     /gene="waaO"
                     /locus_tag="ACHUU3_003644"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HFR2083620.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPFMRPINSNYARYCAK"
BASE COUNT         2103 a         1887 c         2069 g         2126 t
ORIGIN      
        1 gttaacctct gaatatgaac aatttagtgg cgttcatgga attggcgtag cgaaatttgc
       61 ccagttaaag gggattgctg aactggcgcg gcgttactac aacgtgcgga tgcgtgaaga
      121 aagcccttta ctcagcccgg agatgacgcg ggaattttta caaagccagc tcacgggtga
      181 ggagcgggag atctttatgg tgatctttct cgactcacaa caccgggtta taacgcatag
      241 ccgtcttttt tccggcacgc taaaccatgt tgaagtccat cctcgggaaa ttatccggga
      301 agcgataaaa ataaacgcct cggcgctgat ccttgcacat aatcaccctt cgggttgtgc
      361 tgaacccagt aaagcggata aactcattac tgaacggata ataaagagtt gtcagttcat
      421 ggatttacgc gtgctcgacc atatcgtgat tgggcgtgga gagtatgttt cttttgccga
      481 acgcggctgg atttaacccg ctatgcgcga tccttcggga tctttgtctg ttcgggactt
      541 gagcacatcg ctgagtcagc gtatactacg ccacctttga gaatctcggg tttggcattt
      601 gggcctggca atcgagagtt cacatagaac tgcgatgacc gggctgtaaa gcctgacgag
      661 gcgccaatac cccatacgaa gctcgagcta atttgatttt tggagaatag acatgtcccg
      721 agtctgccaa gttactggca agcgtccggt gaccggtaac aaccgttccc acgcactgaa
      781 cgcgactaaa cgccgtttcc tgccgaacct gcactctcac cgtttctggg ttgagagcga
      841 aaagcgtttt gtcaccctgc gcgtatctgc taaaggtatg cgtgtaatcg ataaaaaagg
      901 catcgataca gttctggctg aactgcgtgc ccgtggcgaa aagtactaag tacttagagg
      961 aaataaatca tggctaaagg tattcgtgag aaaatcaagc tggtttcttc tgctggtact
     1021 ggtcacttct ataccactac gaagaacaaa cgtactaagc cggaaaaact ggaactgaaa
     1081 aaattcgatc cagttgttcg ccagcacgtg atctacaaag aagcgaaaat caaataattc
     1141 tcgctttgat gtaacaaaaa acctcgctcc ggcggggttt tttgttatct gcttgccccc
     1201 atattgattg catctgttca ttcctggaga tgctatgcct gaattacccg aagttgaaac
     1261 cagccgtcgc ggcatagaac cgcatctcgt tggtgcaacc attcttcatg cggtggtgcg
     1321 caacggacgc ttgcgctggc cggtttcaga agagatctac cgtttaagcg accaaccagt
     1381 gcttagcgtg cagcggcggg ctaaatatct gctgctggag ctgcctgagg gctggattat
     1441 cattcatttg gggatgtctg gcagcctgcg catccttcca gaagaacttc cccctgaaaa
     1501 gcatgaccat gtggatttgg tgatgagcaa cggcaaagtg ctgcgctaca ccgatccgcg
     1561 ccgctttggt gcctggctgt ggaccaaaga gctggaaggg cacaacgtgc tggctcatct
     1621 tggaccggag ccgcttagcg acgatttcaa tggtgagtat ctgcatcaga agtgcgcgaa
     1681 gaaaaaaacg gcgattaaac cgtggctgat ggataacaag ctggtggtag gggtagggaa
     1741 tatctatgcc agcgaatcac tgtttgcggc ggggatccat ccggatcggc tggcgtcatc
     1801 actgtcgctg gcagagtgtg aattgttagc tcgggtgatt aaagcggtgt tgctgcgttc
     1861 gattgagcag ggtggtacaa cgctgaaaga ttttctgcaa agtgatggta aaccgggcta
     1921 tttcgcgcag gaattgcagg tttacgggcg aaaaggtgag ccgtgtcggg tgtgcggtac
     1981 gccgattgtg gcgactaaac atgcgcagcg ggcaacgttt tattgtcggc agtgccagaa
     2041 gtaattcata cgcgccggat ggcataccat ccggcataaa cgctacgcta acttcgccac
     2101 cagcgcctga tggacattct ccggcaggaa atgggtgaca tcgccctgat ggcgcgccac
     2161 ctctttcacc aacgatgaag agataaacga ccactctttc gacggcatca gaaacacact
     2221 ttccagttcc ggcattaagt ggcgattcat atgcgccagc tgcatttcat attcaaaatc
     2281 cgccaccgca cgcaggccac gaatcagcac cgtagcgtgt tgattacggg cgaagttcgc
     2341 catcaaatca ctaaacccaa ccacttccac gttccccaga tgcgcggttg cctgctgtgt
     2401 cagcgccaca cgctcttcca gggtaaacat cggtttttta ctggggctgg cggcaatcgc
     2461 cagaataacg tgatcgaaca tttgcgtggc gcgcgtcacg atatcgatat gaccattggt
     2521 aatgggatcg aaagtacccg gataaatcgc ccgtttttgc ataacaacct caatgcgttt
     2581 tcggtggcag gtaaggttcc agcagttgaa gcagacgctg tagcgcgccc tggttttgat
     2641 acagtacttc aacggcatga cggccataga aactacggta atcggcgtcg gtgagtaaag
     2701 aggaaacctc ttttgcaagc gtagtggcat cggtaacggt aatcagcccg cttgcctgct
     2761 ccagccgcgc gcaaatgtct ttgaaattaa aagtatgcgg ccccatcaat accggaatag
     2821 cgtgtgcggc agcttccaga ggattatgcc ctccacgttc aaccagtgaa ccgccaacaa
     2881 aggcgagatc ggcaatgccg tacagtaaca tcaactcgcc catcgtatcg ccaaccacaa
     2941 cctgcgtgct ggtggagggg acttcccctg aagagcgtgt gatatagctt agtccagcct
     3001 ggcggacaag gttaatcgca tccgggaagc gttctggatg acggggtacc aggatgagca
     3061 ataaattcgg gaattgctgt aataatgcct gatgtgcggc gatcaccaca ctctcttcac
     3121 cttcgtgagt gctggtggca atccataccg ggcggtgtgg tgcccactgg cggcgcagcg
     3181 tcacggcttt agcagccaac tgcggcgtta cagaaatatc gaatttcagg ctaccggtaa
     3241 cggttacctg gttatttttt gcgcccagcg ccacaaaacg tgcaccatct tcttcatttt
     3301 gcgcagcaat cagcgtaata cgacgcagta agcggcggac gaatttaccc agtttggcat
     3361 aacctgcggc cgagcgggca gagagtcgcg cgttagcgat caccagcgga attttacgtt
     3421 tatgtagcgc cgcaatcagg ttaggccata gttcggtttc cataatcaac accagtttgg
     3481 ggtcgacttt attcaggaaa cggttgagcg catcgggcag gtcatacggc agataaacgt
     3541 gctgaacatc cttcccgaaa gccgattgta cgcgctccga accggttggc gtcatggttg
     3601 taacggtaat cggtaaatca ggataacgat ggcgcagcgc gcgcaccaac gggatcgccg
     3661 ccagagtttc accgacggag acggagtgca gcataatgcc gcctggtttt agcggatggc
     3721 ggtaaaaacc gtaacgttca ccccagcgtt ttcgataggc cggagcctta cgtccgcgca
     3781 cccagagccg tatccagatc agcggctgaa taaggtagag aagggcggtg taaagcaatt
     3841 cgagcatagt aaatagctga cttatggatg tgctggggat tctatgtatt tagctgtggc
     3901 tttaccatta cttttcccgt ttttgactta aatagcaatt tacccgccaa ttcttgctga
     3961 acatctatac tttttttgta tttttatgcg attcattgat ttctgctgta cgtgcaggaa
     4021 ggaatcgcag agcaacagtg aaagaatatg gttgctaatg attttatttg attgatttct
     4081 ttattgaaaa tcaacaatgt gcttaaaaag cattcgggtc ggattgccgc gaaagaagtg
     4141 atacactagc gcgtcaatat cagtgcggta tcagtattca ggtagctgtt gagcctgggg
     4201 cggtagcgtg ctttttctgc ttcaccttaa tcggataatc tcaacaaaaa gagttacttg
     4261 tggataagcc atttctaaga attttgctca ttaagatgcg ttttcatggg gatatgttat
     4321 taactactcc cgtcattagt tcgctgaaaa aaaattaccc tgacgcaaaa atcgatgtgc
     4381 tgctttatca ggacaccacc ccgatcctgt ctgaaaatcc agagattaac gcgctctacg
     4441 gcataaaaaa taaaaaagca aaagcctcag aaaaaattgc caactttttt catctcatca
     4501 aggtattacg tgccaataag tatgacctta tcgtcaatct taccgatcaa tggatggttg
     4561 ctatactggt tcgcttatta aatgcccgtg tgaaaatttc ccaggattat catcatcggc
     4621 agtctgcttt ttggcgtaaa agtttcaccc atttagtgcc gttgcagggt ggaaatgtgg
     4681 tggaaagtaa cttatccgtg ctgacaccat tgggacttga ttcgttggtg aagcagacaa
     4741 ccatgagtta cccgcctgca agctggaaac gtatgcgtcg cgaacttgat cacgctggtg
     4801 ttggacaaaa ttatgtggtt atccaaccta cggcgcgaca aatcttcaaa tgctgggaca
     4861 acgccaagtt ttccgctgtg attgatgcct tacatgctcg tggttatgaa gtcgttctga
     4921 cgtccggccc agataaagac gatctggcct gcgtcaatga aattgcgcag ggatgccaga
     4981 cgccaccagt aacggcgctg gctggaaagg tgaccttccc ggaacttggt gcgttaatcg
     5041 atcatgcgca gctgtttatt ggcgttgatt ccgcaccggc gcatattgcc gctgcagtta
     5101 atacgccgct gatatcgctg tttggtgcga cagaccatat tttctggcgt ccttggtcaa
     5161 ataacatgat tcaattctgg gcgggagatt accgggaaat gccaacgcgc gatcagcgtg
     5221 accgaaatga gatgtatctt tcggttattc cggcggcaga tgtcattgct gctgtcgata
     5281 aattactgcc ctcctccacg acaggtacgt cgttatgatc gttgcttttt gtttatataa
     5341 atattttccc tttggcggtt tgcagcgcga ttttatgcgt attgctcaga cagtcgccgc
     5401 ccgaggtcat catgttcggg tttataccca gtcgtgggaa ggcgaatgcc ctgatgtatt
     5461 tgaactgatc aaagtgccgg ttaaatcgca taccaatcac gggcgcaatg cggagtattt
     5521 tgcctgggtg caaaaacatt tacgcgaaca tcccgtcgat aaagtcgttg gattcaacaa
     5581 aatgccgggg ctggacgttt attatgccgc tgatgtttgt tatgccgaga aagtcgcgca
     5641 ggaaaaaggc tttttctatc acctgacgtc acgttatcgc cattatgccg cctttgagcg
     5701 ggcaaccttc gaacagggca agccgacaca gctgctgatg ctgacagata agcaaatcgc
     5761 cgatttccag aaacattatc agactgaagc ggagcgtttt catattctgc caccggggat
     5821 ttatcctgat cgtaaatata gccagcagcc agctaatagc cgtgaaatct tccgtaagaa
     5881 gaatggaata accgaacaac aatatttatt gttgcaggtc ggttcagact tcacgcgtaa
     5941 aggtgtcgat cgttccattg aagcacttgc ttcgttaccg gattcgctgc gccacaacac
     6001 attgctatat gttgttggac aggataagcc gcgaaaattt gaggcgctgg cagaaaaacg
     6061 cggcgtgcgc agtaatgttc acttcttctc ggggcgcaac gatgtctcgg aattaatggc
     6121 ggcggcggat ttattactgc atcctgccaa ccaggaagca gcgggaattg tgctactgga
     6181 agcgattact gcaggattac cggtactaac aacagccgtt tgtggctatg cgcattatat
     6241 tgtcgacgcc aattgcggcg aggctattgc tgagccattc cgccaggaaa cattgaatga
     6301 gattttacgc aaagcgttaa cgcaatcttc attgcgccag gcctgggcgg aaaatgcgcg
     6361 acattatgct gatacacaag atttatacag tctgccagag aaagcggcgg atatcataac
     6421 gggtggtctg gatggttgaa cttaaagagc cgtttgccac gttatggcgc ggtaaagatc
     6481 cttttgagga agttaaaacc ttgcagggtg aggtttttcg tgaactggaa actcgccgca
     6541 ctctgcgatt tgaaatggcg ggcaaaagct attttctcaa atggcatcgc ggcacgaccc
     6601 tgaaagagat aatcaaaaat ttactctcat tgcggatgcc agtattaggc gcagaccgcg
     6661 aatggaatgc gattcatcga ctgcgggatg tcggcgttga tactatgtat ggggtggcat
     6721 ttggcgaaaa aggtattaat ccgctgagca gaacctcgtt tattattacc gaagatctga
     6781 ccccaaccat cagtctggaa gattactgtg ctgactgggc gaccaaccca ccagatgttc
     6841 gcgtaaagcg tatgcttatt aagcgtgtcg cgacgatggt gcgcgatatg catgctgtgg
     6901 gcattaacca ccgtgactgt tatatctgtc atttcctgct gcacttgcct ttttccggta
     6961 aggaagagga gttaaaaatt tcggtaattg acctgcaccg ggcgcagctt cgcacgcgcg
     7021 ttccacgtcg ttggcgcgat aaagatctta ttgggcttta tttttcttcg atgaatatcg
     7081 gcctgactca gcgggatatc tggcggttta tgaaagtgta ttttgtcgcc ccgcttaaag
     7141 acattctcaa gcaggaacaa ggactgctgt cgcaagcaga agcaaaagcc acaaaaatca
     7201 gggaaagaac gattcgaaaa tcgttgtaat ttccggagag aaatatgagt gcccactatt
     7261 ttaatccaca agagatgatc aataagacaa tcatcttcga tgaaaggcca gcggcgtcag
     7321 tggcatcatc attccatgtt gcttatggca ttgataaaaa ctttcttttt ggttgtggtg
     7381 tttcaatcac gtcagttttg ttacataaca acgacgtgag ttttgttttc cacgttttta
     7441 ttgatgatat ccctgaagcc gatatccagc gtttagccca attggcgaaa agctatcgta
     7501 cctgtatcca gatccatctg gtaaattgtg aacggcttaa ggcattaccg acgaccaaaa
     7561 attggtctat tgccatgtat ttccgttttg taattgcaga ttactttatt gatcaacaag
     7621 ataagatcct ttacctggat gctgatatcg cctgtcaggg aaacttaaag ccgctgataa
     7681 caatggatct tgccaataac gttgctgctg ttgttactga acgcgatgct aactggtggt
     7741 cgttacgggg tcaaagtctg cagtgtaatg aacttgaaaa gggttacttt aattcaggtg
     7801 tcctgttaat taatacacta gcgtgggcgc aggagtccgt ttctgctaaa gcgatgtcga
     7861 tgcttgctga taaagccgtc gtttcccgtt taacctatat ggatcaagat atacttaatc
     7921 ttatcctgtc agggaaagtt aaattcattg atgctaaata caatactcaa tttagtttaa
     7981 attatgaatt aaaaaaatca tttgtttgtc caattaatga tgaaaccgta ttaattcatt
     8041 atgtcggccc gacaaaaccc tggcattact gggccggtta tccaagtgcg caacctttta
     8101 tcaacgccaa agaagcatcg ccctggaaaa atgaaccgtt tatgcggcca attaattcaa
     8161 actatgctcg ttattgcgcc aagca
//
LOCUS       DAZPLI010000171         8182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZPLI010000171 DAZPLI010000000
VERSION     DAZPLI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.172"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHUU3_003645"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHUU3_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFR2083621.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHUU3_003646"
     CDS             complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHUU3_003646"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR2083622.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHUU3_003647"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHUU3_003647"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR2083623.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8182
                     /locus_tag="ACHUU3_003648"
     CDS             6679..>8182
                     /locus_tag="ACHUU3_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFR2083624.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1979 a         2031 c         2321 g         1851 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact cggcggttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 ccacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cg
//
LOCUS       DAZPLI010000172         8156 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZPLI010000172 DAZPLI010000000
VERSION     DAZPLI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.173"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..254
                     /locus_tag="ACHUU3_003649"
     CDS             <1..254
                     /locus_tag="ACHUU3_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR2083625.1"
                     /translation="RGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRY
                     FLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(303..1559)
                     /gene="nupG"
                     /locus_tag="ACHUU3_003650"
     CDS             complement(303..1559)
                     /gene="nupG"
                     /locus_tag="ACHUU3_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR2083626.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="ACHUU3_003651"
     CDS             complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="ACHUU3_003651"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR2083627.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(2905..3180)
                     /locus_tag="ACHUU3_003652"
     CDS             complement(2905..3180)
                     /locus_tag="ACHUU3_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR2083628.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="ACHUU3_003653"
     CDS             complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="ACHUU3_003653"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR2083629.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4421..5140
                     /gene="trmB"
                     /locus_tag="ACHUU3_003654"
     CDS             4421..5140
                     /gene="trmB"
                     /locus_tag="ACHUU3_003654"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR2083630.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5140..5466
                     /locus_tag="ACHUU3_003655"
     CDS             5140..5466
                     /locus_tag="ACHUU3_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR2083631.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5650..6369
                     /locus_tag="ACHUU3_003656"
     CDS             5650..6369
                     /locus_tag="ACHUU3_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR2083632.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6545..7591
                     /gene="ansB"
                     /locus_tag="ACHUU3_003657"
     CDS             6545..7591
                     /gene="ansB"
                     /locus_tag="ACHUU3_003657"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR2083633.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            7708..>8156
                     /locus_tag="ACHUU3_003658"
     CDS             7708..>8156
                     /locus_tag="ACHUU3_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR2083634.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFN"
BASE COUNT         2087 a         1987 c         2161 g         1921 t
ORIGIN      
        1 ttcgcggtga agtggagttg gtgcggattc gtgatgccga agggcgaatt gcggcagaag
       61 gggcgttgcc ttatccacct ggcgtgcttt gcgtggttcc cggggaagtc tggggtgggg
      121 cggttcaacg ttatttcctt gcgctggaag aaggcgtgaa tttgttgccg ggattttcgc
      181 cggagttgca aggtgtctat agcgaaaccg atgcggatgg catgaaacga ttgtacggtt
      241 atgtgttgaa gtaagaataa aaaaacgggt caccttctgg cgacccgttt ttctttgcgt
      301 aattagtggc taaccgtctg tgtgcctgtc gggacacgaa cgtgtttata tttgaacatc
      361 gccatgaacg cgaaggccag aaccacggaa taaccagcga aaatcaacca tacggtctgc
      421 cagtcggtaa tgccgttttg ggtgtacatc tcaacaactt taccgctcac gatgccgccg
      481 aggatacagc cgaagccgtt agtcatcatc aggaacatcc cttgtgcact ggcgcgaatt
      541 gccgggctaa cttctttttc gacaaacacc gaaccagaga tgttgaagaa gtcgaatgcg
      601 caaccgtaaa cgatcatcga cagtaccagc agtacagtac cgaacggagt cgggtcgccg
      661 taagcaaaca gcgcaaaacg caggatccac gccacaatac tgatcatcat tacgttctta
      721 ataccgtagc ggcttaagaa gaacgggatg gtcagaatga acagggtttc agagatctgc
      781 gaaatcgaca tgatgattga cgcatgctgc acgatgaagc tgctggcaaa catcggatct
      841 ttgtcgaagc tgtgcaggaa ggtattaccg aacatgttgg taatctgcag ttccgcgccc
      901 agcagcattg agaagatgaa gaagattgcc atacgcttgt ttttaaacag cgcgaatgca
      961 tcgaggccca gcagggttgt ccagctctga ttcgcttgct gtttagcaac cggaatatgc
     1021 ggcagagtca gggtaaacag aaccagaatg gcggaaagtg ctgcgccaat atacagctgc
     1081 atgtggctta attcgaagcc agacaggctc accacccaca ttgccatgat aaagccgatg
     1141 gtgccccaga tacggattgg cgggaagtca gtaacgatat ccatcccggc attttgcagg
     1201 cgatagtaag agatggtgtt gattaaccca agcgttggca tataagcaaa cgagttaatc
     1261 aatatcacaa ggaacatcgc ttccggcgtc gtgacctgtg ccgccatgaa cagcgtgata
     1321 gcgccaatgg tgtggcaaat ggcatatacc catttcgcac ttaaccattt gtcggccaca
     1381 atccccagca gcgcaggcat aaataccgcg gcgataccca gtgagctata aactgcgcca
     1441 atagaagcac cgtcaaactt cagggtaaca aacatatagg agccgagggt cgtcagccaa
     1501 cttccccaca gacagaactg cagaaaagag aggattttca gctgcagctt aagattcatg
     1561 ttaatttcct cacattgtga tgcggatgag tgtttgaaaa agcacatttg accgtgcgag
     1621 atgatgggat tctatcaact tcgcggactg gttttttgtt acctgtggca aataattgca
     1681 aacattccca atttgcaaaa cgaaattgtg atgtggataa catttttgcc cctgagcatc
     1741 gtcaggggcg gttaatggaa ttatcggcgg cggtaggatt tttgcgcggt attcacttta
     1801 taaagataac ggcgagattc cgcagaggga tggcgggtcg tcagcgtctg ataaacatcg
     1861 cccggcgtca tggtgttaat aatattggca gcctgaatct tatcattcga aaagactcgc
     1921 agcacgctgc ctgcgccgcc gttataggcg gtgatgacgg cataacgccg cgatgttggg
     1981 ttatcaattc cgccgagata aacattgttc agcatcgcca gatacgcggt gccggtatca
     2041 atattgctgg caggatcaaa caagaaactg cggctcggcg tgccggattt cccctgcgag
     2101 cggaacacat ctttcccggc agtatgttgt accacctgca ttaatcccag cgcatcggaa
     2161 cggctgaccg catacgggtt aaaggaagat tcggtctgca taattgccag aatcagcgac
     2221 tcatcaacgc catatttccg tgacgcctgg cggaccatgc cgagatattt gtgcgcacgt
     2281 ttatcaaggt ggttcggtac catgttaatg gtgacgctgt agatgatacg cagcccgtta
     2341 ctgcggcttt gcagacggtt tttcagcaga taatccgcga agttgcttgc gcgaccttcc
     2401 cagcgaatcg gctgcccggt gttgtccacc acctgaccgt aaaggaaagg ttctttcgaa
     2461 atcgtaatat catcaacgtc ggaatagaga tcgaccgaac tcggatcgtc acccatcagc
     2521 aacgttttga taattgcccg gcgcaaatgc gcggcaggtt ctgtcccggc gatagtttcg
     2581 atagtaatcg taccgtcatc gaagttgatg tggctgcggg tctgatattg atcggtgtat
     2641 ttcacgtagt ccttaggacc ggcgatcacc acctctttga atccccagat gttctcaata
     2701 ttgtgggcaa attgccccat cagaatatca aaaccgttgg tatctttgac ccaggcttcg
     2761 ttataggtat cgcctttttt ggtcgtcgaa caggagatga gcaacggcgc aatcaaagcc
     2821 agcgcgagat attttttcat cattccgggt gcgtgttgtg tttgttttta tgcaagttgc
     2881 cggaggcgtg ctccggcact gtttttattt tttatcttcc ggcgtatagc cctcgatatg
     2941 cacctcttta ccctcgaaca ggaagttgac catctcctgc tcaagcagct tgcggtgctc
     3001 ggcattcatc atgttgagtt tcttttcatt aatcagcatg gtttgcttgt gctgccactg
     3061 cgcccaggct tctttggaga tctcgttata gatgcgtttt cccagctcgc cggggtacag
     3121 ctgaaaatcc tgaccttctg cttcacgttg caggaaagta caaaaaatcg ttctgctcat
     3181 aaatcatcct ctttatcgac tcacgcgcta aaccggcgcg ccagtgcgta actgctgtaa
     3241 caaacgctcc acgggagccg ccaggccaac tgacggcggt tgcgctaagt tataccagag
     3301 cgcattgcct tcatccatgc agccggtgaa tgacgacacg ggaagccaca taggcacaat
     3361 atctaagtgg aaatggctga aggtatgccg aaacgcggtc agttgcgtca ggttatcggc
     3421 agcaatctgc cgttgcgcca gccactgccg caaactttct tcgtcggcaa actgcgggaa
     3481 acagtataaa ccgccccaca atccgctcgg cggacgctgc gccagcaata cttcatcttc
     3541 gtgctgtaac agcaaaaagt agccggtgcg ctccggcagc gtctgtttcg gttttttgcc
     3601 cggataaagc gaccagctat tattggcggc ggcaatacat ccgttttgta gcggacagag
     3661 cgaacatttc ggcttcgagc gcgtacaaat catcgcgccc aaatccatca tcgcctgatt
     3721 aaaccgttcc acgccgaccg cgggcgtcac ctgctcgctc aaactccata atttattctc
     3781 gacctctttt ttcccaggcc agccgcttac agcatagcag cgcgccagca cgcgtttgac
     3841 gttaccgtcg agaatcggaa agtgcttacc cagagaaagc gagagaatcg cgcctgcggt
     3901 ggaacgcccg acgcccggta acgccgcgac ttcttcaaag gtttccggga atttaccgcc
     3961 gtgtaaggtc gccacttgtt gtgccgcttt atgcagattg cgcgcgcggg cgtaatagcc
     4021 aagcccggtc cacaagtgga gaacttcgtc gagcggcgca ttggcgagat cggtcaccgt
     4081 cgggaagcgc gccataaagc gttcaaaata ggggataacg gtcgcaacct gagtttgttg
     4141 caacatcact tctgagagcc atactttgta gggcgtcttg tcaatttgcc agggcagcgt
     4201 ttttcgcccg tatttatcgt accagtccag aacctgggct gaaaattgcg acgcttgcat
     4261 ggtcaccgaa ttcactgttg ttgggggcaa gattgcagca tagcgacggc agggtgtaaa
     4321 ccggaacttt ccgcagccac ggccttatca tgcttgcatc cggcaactaa ctttggataa
     4381 tgcccgtttt cagaacactt tcacaagcga ctaaatcttt atgaaaaacg acgtcatttc
     4441 accggaattt gatgaaaacg gccgcccact gcgccgtatc cgtagttttg tgcgccgcca
     4501 ggggcgactg accaaaggcc aggaacatgc gctggaaaac tactggccgg tgatgggcgt
     4561 tgagttcagc gaagatatgc tggatttccc cgcgcttttt ggccgtgaag cgccggtgac
     4621 gcttgagatt ggttttggca tgggggcgtc gctggtggca atggctaaag atcgccctga
     4681 gcaggacttc ctcggcattg aagtgcattc accgggcgtt ggtgcgtgcc tggcttctgc
     4741 gcatgaagaa ggtttaagca acctgcgcgt gatgtgtcac gatgcggttg aagtgctgca
     4801 taaaatgatt cctgacaatt cattgcgcat ggtgcagctc tttttccctg acccgtggca
     4861 caaagcgcgc cataataaac gccgtatcgt acaggtgccg tttgccgaac tggtaaaaag
     4921 caaactgcag ctggggggtg tattccatat ggcgaccgac tgggaacctt atgcggaaca
     4981 tatgcttgaa gtgatgtctt ctattgacgg ttataaaaac ctgtcagaga gcaatgatta
     5041 cgtgccgcgt ccggcatcac gtccggtgac gaaatttgaa caacgtggtc atcgtcttgg
     5101 tcacggagta tgggacttaa tgttcgagag ggtgaaataa tggcaaagaa ccgtagccgt
     5161 cgtctgcgta aaaaaatgca catcgacgaa ttccaggaat taggattttc ggtggcatgg
     5221 cgattcccgg aaggtacatc ggaagaacag attgataaaa ccgttgatga ttttattaac
     5281 gaggttatcg aaccgaacaa actggccttt gacggcagcg gttatctggc ctgggaaggt
     5341 ctgatctgca tgcaggaaat cggcaaatgc accgaagaac atcaggcgat tgtgcgtaag
     5401 tggctggaag agcgcaaact ggatgaggta cgcaccagcg aacttttcga cgtttggtgg
     5461 gactaagaaa gcatacgggc gatgacaaat gcaaaactgc ctgatgcgct acgcttatca
     5521 ggcctggaaa gatgcacgat cgagtaggcg ggataaggtg tttacgccgc atccggcatg
     5581 gaaaacgcgt actttgttat caatctgggg ccagcaaatg ctggcctgat tttttcttga
     5641 gggaagacta tgatgcgcaa aatgctgctg gcggcagcac tttcagtgac ggcaatgacc
     5701 gctcacgccg actaccagtg cagcgtcacg ccgcgtgacg atgtgattgt cagcccgcaa
     5761 accgtgcagg tgaagggcga aaacggcaat ctggtgatca cgccagacgg caacgtgatg
     5821 tataacggta agcaatattc cctgaatgcc gcccagcgcg agcaggcgaa ggattatcag
     5881 gctgaactac gcagcacgct gccgtggatt gatgaaggcg cgaaaagccg cgtcgaaaaa
     5941 gcccgtattg cgctggataa aattatcgtt caggagatgg gcgaaagcag caaaatgcgc
     6001 agccgtctga ccaaacttga tgcgcagctg aaagagcaga tgaaccgcat tatcgaaacg
     6061 cgcagcgatg gcctgacgtt tcactataaa gccattgatc aggttcgcgc cgaaggccag
     6121 caattagtga atcaggcaat gggtggaatt ttacaggaca gcattaatga aatgggcgcg
     6181 aaagcggtgc tgaaaagcgg cggtaaccca ttacagaatg tgctgggaag cctggggggg
     6241 ctgcaatcct caatccaaac agagtggaaa aagcaggaaa aagatttcca gcagtttggc
     6301 aaagatgttt gtagccgcgt tgtgactctg gaagatagcc gcaaagccct ggtcgggaat
     6361 ttaaaataat cctctatttt aagacggcat aatacttttt tatgccgttt aattcttcgt
     6421 tttgttacct gcctctaact ttgtagatct ccaaaatata ttcacgttgt aaattgttta
     6481 acgtcaaatt tcccatacag agctaaggga taatgcgtag cgttcacgta actggaggaa
     6541 tgaaatggag tttttcaaaa agacggcact tgccgcactg gttatgggtt ttagtggtgc
     6601 agcattggca ttacccaata tcaccatttt agcaaccggc gggaccattg ccggtggtgg
     6661 tgactccgca accaaatcta actacacagc gggtaaagtt ggcgtagaaa atctggttaa
     6721 tgcggtgccg caactaaaag acattgcgaa cgttaaaggc gagcaggtag tgaatatcgg
     6781 ctcccaggac atgaacgata atgtctggct gacactggcg aaaaaaatta acaccgactg
     6841 cgataaaacc gacggcttcg tcattaccca cggtaccgac acgatggaag aaaccgctta
     6901 cttcctcgac ctgacggtga aatgcgacaa accggtggtg atggtcggcg caatgcgccc
     6961 gtccacgtcc atgagcgcag acggtccatt caacctgtat aacgcggtag tgaccgcagc
     7021 tgataaagcc tccgctaatc gtggcgtgct ggtggtgatg aacgacaccg tactggacgg
     7081 tcgcgatgtc accaaaacca acaccaccga cgtagcgacc ttcaagtctg ttaactacgg
     7141 tcctctggga tacattcaca acggtaagat tgactaccaa cgtaccccgg cacgtaagca
     7201 caccagcgat acgccattcg atgtctctaa gctgaatgag ctgccgaaag tcggcatcgt
     7261 ttataactac gctaacgcat ccgatcttcc ggctaaagca ctggtagatg cgggctatga
     7321 tggcatcgtt agcgctggtg tgggtaatgg taacctgtat aaatccgtgt tcgacaccct
     7381 ggcaaccgcc gcgaaaaacg gcactgcagt agtgcgttct tcccgcgtac cgacgggtgc
     7441 taccactcag gatgctgaag tggatgatgc gaaatacggc ttcgtcgcct ctggcacgct
     7501 gaacccgcaa aaagcgcgcg tcctgctgca gctggctctg acgcaaacca aagatccgca
     7561 gcagatccag cagatcttca atcagtacta atcacctcgc cccggtatcg tgccggggct
     7621 ttttcacttc agacttacgt ccattgccaa ttttcattac cctaatgata atcaccggaa
     7681 taaattattc cgcgtgaggt ttttcgggtg aaaaagcaat ggattgttgg cacggcactg
     7741 cttatgttga tgactggtaa tgcctgggca gatggcgaac cgccaactga aaatatctta
     7801 aaagatcaat tcaaaaagca gtatcacggc attctcaagc ttgatgctat caccttaaaa
     7861 aatcttgatg ctaagggtaa tcaggccacc tggtcagcgg aaggcgatgt ctcttccagt
     7921 gacgatctct atacctgggt cggtcagttg gcagattacg aactgctcga acagacctgg
     7981 acgaaagata aaccggtaaa attctcggcg atgttaacca gtaaaggaac gcccgcgtct
     8041 ggctggtcgg tgaactttta ctcttttcag gcggcagcca gcgatcgtgg gcgggtggtc
     8101 gacgatatca aaacgaataa taaatatctg atcgtgaata gcgaagattt caatta
//
LOCUS       DAZPLI010000173         8043 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZPLI010000173 DAZPLI010000000
VERSION     DAZPLI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8043
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.174"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            321..458
                     /locus_tag="ACHUU3_003659"
                     /pseudo
     CDS             321..458
                     /locus_tag="ACHUU3_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904967.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            812..1054
                     /gene="yiiF"
                     /locus_tag="ACHUU3_003660"
     CDS             812..1054
                     /gene="yiiF"
                     /locus_tag="ACHUU3_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR2083635.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="ACHUU3_003661"
     CDS             complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="ACHUU3_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR2083636.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="ACHUU3_003662"
     CDS             complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="ACHUU3_003662"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR2083637.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="ACHUU3_003663"
     CDS             complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="ACHUU3_003663"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR2083638.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="ACHUU3_003664"
     CDS             complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="ACHUU3_003664"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(6320..6322),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR2083639.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7101..7934
                     /gene="fdhD"
                     /locus_tag="ACHUU3_003665"
     CDS             7101..7934
                     /gene="fdhD"
                     /locus_tag="ACHUU3_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR2083640.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1864 a         2066 c         2088 g         2025 t
ORIGIN      
        1 atttacatca ttccctgtct atgccggtaa tattgaataa gaactctata aatttttatt
       61 cttcgttggc accatacgtg tcttgctaca tcactgttca ggagcattta aatattattt
      121 gtagagctga gtggcaatta tagcagataa tgcttaactc atcatcttag ttcaggtttg
      181 agcgattttt gatagtcaaa acctcctctg gtgccatact tttctcataa atatttttaa
      241 aatctatagc tcacaaacat tatgcaaaga taatgggctg gcatcattgt cagtatcatt
      301 catttactgc tctgtatgtt atgaatcatt atgttgtatg tttgctctta aaccgtggga
      361 aggaggctat aatggttgcc aatattaatc tcattaaaca agaaagttat tctgttgtta
      421 atttagaaaa acaactttcc cgaactgtta ccaacgaaat tataacgatg agcaagtaaa
      481 gaccggttgg tggcagtact gaccggtctg tgatgaaaga ttaatttcac cttaaatcct
      541 ggtgctgtta ggatgctgac gtttttcggc tacaccattc agattatgca aataaatatc
      601 cctgaatata atatattcat agcacccata actcatcctg ggcgataact aaaccatttt
      661 tgagtaataa atgaaacaac tgcaacgctc ttattatttc tggaaaacgc ctcgcaaaaa
      721 taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact tttctctcca
      781 tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta tagatatggg
      841 cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc tcgaggtgca
      901 gcgtaatctt cctcgtgcgg acctattaag ggaagcggta gatcaatacc tgataaatca
      961 atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg aagatggtgt
     1021 cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt caatcagatt
     1081 gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
     1141 agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc agcgcatcag
     1201 gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt tggagggaag
     1261 aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa tacgagagat
     1321 ggtgtcgtca caccctatga gctgtaatcc gggcgacctg cgccacccgg aaaacgtcag
     1381 agattactct ccttcacccg gaaacaggaa cgggttaatg gaactacggg cgtagccttc
     1441 ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca tctgccacgg cttcaacttt
     1501 cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc agctttcggc
     1561 tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc tctgctcgca
     1621 gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc agtgcaggta
     1681 acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag agccacatac
     1741 cgggcaatat tgacgttgtt cgccgtattc agcgcgggct ttgccgggga tcagattggc
     1801 catctgcgcc cagtagagcg acagcgcagc ccagataaac ggcgctttat cgctgctgac
     1861 ggacgagaaa tcagaggcaa acagcgcgct ggccatatct tccagctcct gagtcgatgc
     1921 cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt tcagctcagc
     1981 aattagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt gaatatccag
     2041 cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca tctccagcgg
     2101 atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc gcaggtaatc
     2161 acccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt tgtataaatt
     2221 cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct cgctcgaacc
     2281 cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt tgtcttgcgg
     2341 acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac ccatccttcc
     2401 accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac catgataacc
     2461 acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat caccgggatt
     2521 gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag cagcagaacc
     2581 aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg cccggtgtca
     2641 cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat atcatcccga
     2701 ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc aaacataacc
     2761 acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat ttgcatcaac
     2821 cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat gaagcagaag
     2881 gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt gtcacgtcgt
     2941 ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca tccgcacggt
     3001 tcggaccgac acccacgtag tggaagatac tggccgcaaa ggtagccgca aagccaacag
     3061 ccgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt tccgggttct
     3121 ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac atgacgtgtg
     3181 taccaccgac gcctgccgga tcgtacagac ccgcattgtc gtaaccgcgg gttttcagct
     3241 cagcaacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag tgaatcgcgc
     3301 ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg tcaacgcaca
     3361 gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg aacggacagc
     3421 ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg ataccgttgg
     3481 catactgaat aattgcacct tccgccgggc acgctttcag gcagcctgga tcggaacagt
     3541 gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc acttccgaga
     3601 agcgcatcac cgtccacgat ttggcgctta aatcattggg gttgtcgtac accccaatgt
     3661 tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga caggctttac
     3721 agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag tcccgcgcct
     3781 gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttgc gattgataag
     3841 ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta aactccggcg
     3901 tctgcgtgtt cgcatcacca acgaatggcg ttaacgtatt ggcaataaag ccttttttag
     3961 ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct ttgccgttcg
     4021 ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag ccacggttgg
     4081 aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc gactccccga
     4141 tttccacaaa ctgctctggt tgcaaaatcg cgttcaacag cgcgtgtttg gtccagtagt
     4201 ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg gctttaccca
     4261 atgcttcggc gtcgtcttta aagattcgcg cagccgggtt cgagataacg tttggatgca
     4321 gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac ggaccttccg
     4381 ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata aacggcccga
     4441 cgccgctgcc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc cacttagtgc
     4501 cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga tctgcggagg
     4561 cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc agcgtgttac
     4621 cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc ggcgtccagc
     4681 taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat tgggcgaacg
     4741 agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg gtaatatcgg
     4801 ccagcgcctt accgttgctc tccatcgcca cttcttccga cttcggctca tgcggaatgg
     4861 cgtagttcca ggtcatgttc agcacctgat ccgggtttgc gccaccctgt tcggcataca
     4921 tcttgcgcaa gcgcaggaag ataccggaga ggatctcgcc atcagtcagc gcaatccctg
     4981 gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg attgaaccgt
     5041 tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc ttcgacgagt
     5101 caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg ttcagcgggt
     5161 cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt ttgttcggga
     5221 atgaggcaac agggttaaag ccctggcaga tatagccatt gaccttgccc tctttcatca
     5281 tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag tcaaagcccc
     5341 agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag acgaagaatt
     5401 tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc gtgttggcgg
     5461 taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa cctggcaggc
     5521 tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg cgcagggcgt
     5581 taacgccgcc gcctgccatc cccatattgc cgagcagcag ctggatcatc gccatcgtac
     5641 gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg aacgaggcag
     5701 ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac gcgtcttttg
     5761 gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc tgtttcagca
     5821 agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag ccgttttcgt
     5881 ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtca tagccggtga
     5941 acaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg gtataggctt
     6001 cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg cctgacagga
     6061 aagcaatgtc agtaccggaa cgaatagggg catagtagtc agccaccgcc gccgtacgcg
     6121 taaagcgagg atcgatcaca atcagcttcg cgccattgtg aattttggct tccatcgccc
     6181 agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg acgaggttgg
     6241 cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac
     6301 ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc atacccagcg
     6361 cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa gcacacagca
     6421 tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca atgtagttag
     6481 catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc caactgattt
     6541 gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg ctttcggagt
     6601 ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg accgggtgat
     6661 ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg tcaccgaggc
     6721 tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg gtttcgcggg
     6781 tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca aaacccagtg
     6841 ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc cttctgctga
     6901 cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc gcttgctgcg
     6961 tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc cagaattggc
     7021 tcaatttccc tccatccagg aagggtttgt aacagaatac cataatgttg gtgtgtgtgt
     7081 tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa aatgtgacaa
     7141 atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa cacccacggt
     7201 tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt tcgcatgtgg
     7261 tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg ctttccgagg
     7321 ggataataga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc tgtaatggtc
     7381 ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag cgccgccggg
     7441 cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat gacatcggaa
     7501 aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg gatgatgcat
     7561 tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact cacgccgctg
     7621 cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt cgccatgtgg
     7681 cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag caaggtgcgg
     7741 tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg tgcggcgtag
     7801 agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct gagcgctgta
     7861 atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc catccgcagc
     7921 gtttgagcaa ttaattaaaa taagtcctgc gatatatatt aaattgcagg acttattcat
     7981 ttcgtgaatt ttattatttt atttataaat aaaacgcgtg tagtgacgtt ttcttttttc
     8041 att
//
LOCUS       DAZPLI010000174         7983 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZPLI010000174 DAZPLI010000000
VERSION     DAZPLI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7983
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.175"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..643
                     /locus_tag="ACHUU3_003666"
     CDS             <1..643
                     /locus_tag="ACHUU3_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR2083641.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            894..2456
                     /gene="hpaB"
                     /locus_tag="ACHUU3_003667"
     CDS             894..2456
                     /gene="hpaB"
                     /locus_tag="ACHUU3_003667"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFR2083642.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2474..2986
                     /locus_tag="ACHUU3_003668"
     CDS             2474..2986
                     /locus_tag="ACHUU3_003668"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR2083643.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3379..5529
                     /gene="btsT"
                     /locus_tag="ACHUU3_003669"
     CDS             3379..5529
                     /gene="btsT"
                     /locus_tag="ACHUU3_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR2083644.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5579..5782
                     /locus_tag="ACHUU3_003670"
     CDS             5579..5782
                     /locus_tag="ACHUU3_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR2083645.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHUU3_003671"
     CDS             5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHUU3_003671"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR2083646.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6827..7723)
                     /locus_tag="ACHUU3_003672"
                     /pseudo
     CDS             complement(6827..7723)
                     /locus_tag="ACHUU3_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT         1835 a         2177 c         2099 g         1872 t
ORIGIN      
        1 tcatgcgttt attacggagt ctgacgccga cggtcatgtg ttgacggtac gggaagatct
       61 gatctggccc ctgctggaag ttctttatcc aggcactcgg gaaaccttcg gcctgccggg
      121 gatttgcctg tcactggcag ataaacccga cgaactggcg gcgctggaac actattggca
      181 actgatagag cgggaatcgg tggaacaact gcctggacgg gaacacaccc tgacgctact
      241 ggcacaggca gtgttcaccc tactgctgcg taatgcaaaa ctcgacgacc atgccgccag
      301 cgggatgcgc ggagaattaa aactgttcca gcgttttaat atgctgattg aaagccattt
      361 tcatcagcac tggacagtac cggattacgc taacgaactg catatcaccg aatcacgcct
      421 cacggacatc tgccgccgct ttgccaaccg tccgccaaaa cggttgattt tcgacaggca
      481 gctgcgagaa gccaagcggc tgctgctgtt ttctgataac gccgtgaaca atattgcctg
      541 gcaactcggt tttaaggatc cagcttattt tgcgcgcttt tttaatcgct tagtcggttg
      601 ctcgcccagt gcttatcgtg ccaaaaaagt acctgtgacg tgaaaatccc ttagccttaa
      661 cgggaaacca ggcaccacct gctattcccc tttctccgcc tggagcaagg ggatttagta
      721 acgtcaaaaa tcgctaaaag cgaccgcgat cacaaaaccg tacccgacct gaaaagtacc
      781 agccattgtc ccaaaagtgt cttcccgtaa tcgcccctgc aagcttcaat tgaataacaa
      841 aaacaacaca caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac
      901 cagaagattt ccgcgccagt acccaacgtc cgttcaccgg ggaagagtat ctgaaaagcc
      961 tgcaggatgg tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc actactcatc
     1021 cggcatttcg taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg
     1081 agatgcagga ctctctgtgc tggaacaccg acaccggcag cggcggctat acccataaat
     1141 tcttccgcgt ggcgaaaagt gccgacgacc tgcgccagca acgtgacgcc atcgctgagt
     1201 ggtcacgcct gagctatggc tggatgggcc gtaccccaga ctacaaagct gctttcggtt
     1261 gcgcactggg cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt
     1321 acacccgtat tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg
     1381 atcgtcattt gccgaccgat aaagtgaaag acgtttacat caagctggaa aaagagactg
     1441 acgccgggat tatcgttagc ggtgcgaaag tggttgccac caactcggcg ctgactcact
     1501 acaacatgat tggcttcggc tcggcacaag taatgggcga aaacccggac ttcgcactga
     1561 tgttcgttgc gccaatggat gccaatggcg tcaaattaat ctcccgcgcc tcttatgaga
     1621 tggtcgcggg tgctaccggc tcgccgtacg actacccgct ctccagccgc ttcgatgaga
     1681 acgatgcgat tctggtgatg gataacgtgc tgatcccatg ggaaaacgta ctgatctacc
     1741 gcgatttcga tcgctgccgt cgctggacaa tggaaggcgg ttttgcccgt atgtatccgc
     1801 tgcaagcctg tgtgcgcctg gcagtgaaac tcgacttcat tacagcactg ctgaaaaaat
     1861 cactcgaatg taccggcacc ctggagttcc gtggtgtaca ggccgatctc ggtgaagtgg
     1921 tggcgtggcg caacaccttc tgggcattga gtgactcgat gtgttctgaa gcgacgccgt
     1981 gggtcaacgg ggcttattta ccggatcatg ccgcactgca aacctatcgc gtactggcac
     2041 caatggccta cgcgaagatc aaaaacatta tcgaacgcaa cgttaccagt ggcctgatct
     2101 atctcccttc cagtgcccgt gacctgaaca atccgcagat cgaccagtat ctggcgaagt
     2161 atgtgcgcgg ttcgaatggt atggatcacg tccagcgcat caagatcctc aaactgatgt
     2221 gggacgccat tggcagcgag tttggtggtc gccacgaact gtatgaaatc aactactccg
     2281 gtagccagga tgagattcgc ctacagtgtc tgcgccaggc acaaagctcc ggcaatatgg
     2341 acaagatgat ggcgatggtt gatcgctgcc tgtcggaata cgaccagaac ggctggactg
     2401 tgccgcactt gcacaacaac gacgatatca acatgctgga taagctgctg aaataacgca
     2461 gcaggaggtt aagatgcaat tagatgaaca gcgcctgcgc tttcgtgacg cgatggccag
     2521 cctgtcggca gcggtaaata ttatcaccac cgagggcgac gccggacaat gcgggattac
     2581 ggcaacggcc gtctgctcgg tcacggatac accaccatcg ctgatggtgt gcattaacgc
     2641 caacagtgcg atgaacccgg tttttcaggg caacggtaag ttgtgcgtca acgtcctcaa
     2701 ccatgagcag gaactgatgg cacgccactt cgcgggcatg acaggcatgg cgatggaaga
     2761 gcgttttagc ctctcatgct ggcaaaaagg tccgctggcg cagccggtgc tgaaaggttc
     2821 gctggccagt cttgaaggtg agatccgcga tgtgcaggca attggcacac atctggtgta
     2881 tctggtggag attaaaaaca tcatcctcag tgcagaaggt cacggactta tctactttaa
     2941 acgccgtttc catccggtga tgctggaaat ggaagctgcg atttaagctc cagccccttc
     3001 acgcattcgt cgaaggggca tttttattgt gatctcaatc acatatccac ccgatgaata
     3061 attcttgccc aaccgctcac cgccattcct gaccgcttac gtccctaaaa taaccactca
     3121 gttatttacc ttactttacg cgcgcgtaac tctggcaaca tcactgcagg atagcggtca
     3181 atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc ggcctatgat taacaaatta
     3241 ctctatgaat tttggtttgc aggaaggcag caagtgagtg aatccccggg agcttacaac
     3301 agtaagtgac aggggtgaac gaacgcaact gccgcacctg taagccaaaa gacgacgagt
     3361 aaaatgccag gttttactat ggatacgaaa aaactattca agcacatacc ctgggtgatt
     3421 ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc
     3481 agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta tcgctactac
     3541 agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt
     3601 attaacaacg acggtctgaa ctacgttccg accaaccgtt acgtgttgtt tggtcaccac
     3661 ttcgccgcta tcgccggtgc tggtccgctg gtcggtccgg ttctcgccgc acagatgggc
     3721 cacctgcctg gcacgctgtg gctgctggcg ggggtagtac tggccggtgc ggttcaggac
     3781 tttatggtgc tgtttatctc ctctcgccgt aacggcgcat ctctcggtga gatgataaaa
     3841 gaagagatgg gaccagtacc ggggactatc gcgctgtttg gctgtttctt aatcatgatc
     3901 atcatccttg ccgtcctggc gctgattgtg gttaaagccc tggccgaaag tccgtggggt
     3961 gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta tgggtatcta catgcgcttt
     4021 atccgtccgg ggcgtgtggg tgaagtctct gtcattggta tcgtgctgct ggttgcctct
     4081 atctacttcg gtggcgtgat tgctcacgat ccgtactggg gtccggcact gacctttaaa
     4141 gacaccacca ttaccttcgc gctgattggc tatgcgtttg tttccgcatt gctgccggtg
     4201 tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc
     4261 ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc gatgacccag
     4321 tacattgacg gtactggccc gctgtggaaa ggtgctctgt tcccgttctt gttcattacc
     4381 atcgcctgtg gtgcggtatc tggcttccac gcactgatct cttccggtac gacgccgaaa
     4441 ctgctggcta atgaaaccga cgcgcgtttc attggctacg gcgcaatgct gatggagtcc
     4501 ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg aaccgggtct ttacttcgcg
     4561 atgaacaccc cgcctgctgg ccttggcatc accatgccta acctgcatga aatgggtggc
     4621 gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc
     4681 gtcagctctt ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt
     4741 ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccaa cgctggcggt aggtatcgct
     4801 cacgtgttcc acaaagtgct gccgatggct gacatgggct tctggtatca cttcggtatt
     4861 ctgttcgaag ccctgttcat cctgaccgcg ctggatgcgg gtacccgttc tggccgtttt
     4921 atgctgcaag acctgctggg taacttcatc ccgttcctga aaaaaaccga ctctctggtt
     4981 gccggtatca tcggtactgc gggctgtgtg ggtctgtggg gctacctgct gtatcagggc
     5041 gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat ctccaaccag
     5101 atgctggcag ccgtagcgct ggtactgggc accgttgtgc tgattaagat gaagcgcacc
     5161 caatacatct gggtaactgt tgttccggct gtatggctgc ttatctgcac cacctgggcg
     5221 ctgggtctga aactgttcag caccaacccg cagatggaag gcttcttcta catggcaagc
     5281 cagtacaaag agaagattgc taacggtact gacctgacgg cgcagcagat tgccaacatg
     5341 aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga gtattctgtt cctgattgtg
     5401 gtgtacagca tcatcttcta cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa
     5461 cgtactgaca aagaaactcc gtacgttcca atcccggaag gcggcgtgaa gatctcttcg
     5521 caccactaac cgtgtttagc cccgcttcgg cggggctttg ttcttatcag agtgaactat
     5581 gtttggtaac ttaggacagg caaaaaaata tctcggccag gcggcgaaga tgttgattgg
     5641 cattccagac tacgacaact acgtcgagca tatgaagacc aaccatcccg acaagccgta
     5701 catgagctat gaagaattct tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa
     5761 aggcggtatg cgctgttgtt aatggagaga gcatgaaccc gattgcagtt accctactca
     5821 ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca tattcttaac gaacaacacg
     5881 gctacgagat tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat gatcaactga
     5941 ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct
     6001 ccaacgagct ggaagacgca ctactcgacc tgctggataa tctcgacaag ggcaatattc
     6061 agttcgaccg tctggtcatt gaatgcaccg gcatggccga tcccggcccg attattcaga
     6121 ccttcttctc ccatgagatt ttatgccagc gctacctgct ggacggggtg attgcgctgg
     6181 tggatgcggt acatgccgat gagcagatga accagttcac catcgcccag tcccaggttg
     6241 gctacgccga ccgtattctg ctgacgaaaa ccgacgtcgc aggcgaagca gagaaactgc
     6301 gtgaacgcct ggcgcgcatc aacgcccgcg caccggtcta caccgttacc cacggcgata
     6361 tcgacctggg ccttctgttc aacaccaacg gttttatgct ggaagaaaac gtcgtcagca
     6421 ccaaaccgcg tttccacttt atcgcggaca aacaaaacga tatttcgtcg attgtggtgg
     6481 aactggatta cccggtagat atcagcgaag tttcccgggt gatggaaaac ctgctgctgg
     6541 aatcggcgga taaactgctg cgttacaaag ggatgctgtg gattgacggc gaacctaacc
     6601 gcctgctgtt ccagggcgtc cagcgcctct acagcgccga ctgggaccgc ccgtggggcg
     6661 atgaaaaacc gcatagcacg atggtgttta tcgggattca gttgccggag gatgagattc
     6721 gggcggcgtt tgcgggactc aaaaagtaac cagccctcca acatcacaat acctgagact
     6781 ttatcaccct catattcctc cagaatatga gggcattcgg gcttaattat ctctgaacgt
     6841 gcaacacttc ctgcgactcc ttatgcgcct ccacatcctg cttcgttaac cagagcgact
     6901 tacgaccaaa attttcgtac attttcagct gatcttcata gtgctgatca actgttccat
     6961 cgggagcaat aaacccactc tgaccgggtg cgaccacatc ccaggcaagc accggacgat
     7021 cgcttgtcgt tggtgagaaa acaatcatat cgttttctgt tccacggttt tgatactccg
     7081 cctgatgacg cgtttcttcc gctgcggcct gcggtacacc aaagaaatta tttgcacgga
     7141 acgttaaggc cattgcaggt gttttccagt tactcacatt attgccatag cgtttggaaa
     7201 gagtctccca ggtatcttcc agcgcagcca acacaacctc ctgctgtggt ttcccagcaa
     7261 acagatcaac cgcctgtggg attggtgatt tgtctccctg caccgcctca tacaaaattt
     7321 ttgctccaac acttatattc agcgaaccag ttgggccgtc ctgggttgtt tcgtagccac
     7381 tggcgctgta ccacttatca aatggcatag gtacggcagc cactacggta cgcttcaaca
     7441 tactggtcag ccaaacgttc aggatggcag agcctggctg ctgccaggtt ttaccatcat
     7501 cattaagcaa attgatgcca tcccaacgtg ttaatgtatc caccaactga cggcgcggat
     7561 cgctctgtgt caaaccagat gtcgctgctt gcagagtagg taaaaaaagc ctcaggttaa
     7621 gatcctgacg actggtttgg cgaataacat cccatgcctg atcagcagtt aagcgtggct
     7681 tttgctcaag cagtcggtcg atctccgtaa cgcgatctgc cccgttactt tattcgtacc
     7741 ccttataatg gggtgttagc cagccagacc cggcatgatt actgccccca gtcgtccatg
     7801 atccgggggg tgatgtcacc gggtctggtg gggcgctggt aaccgctaat aggggtcagg
     7861 tcaggcactt ttgccgggac cgtctgtaac gtggatgccg gtacctgctc cccgtggtta
     7921 tctggttaac ccatatacaa gggagacaga atgaccgaat ccagcgatta cgaatccgtc
     7981 cag
//
LOCUS       DAZPLI010000175         7967 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZPLI010000175 DAZPLI010000000
VERSION     DAZPLI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7967
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.176"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(152..487)
                     /locus_tag="ACHUU3_003673"
     CDS             complement(152..487)
                     /locus_tag="ACHUU3_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR2083647.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="ACHUU3_003674"
     CDS             complement(491..835)
                     /locus_tag="ACHUU3_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR2083648.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHUU3_003675"
     CDS             complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHUU3_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR2083649.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHUU3_003676"
     CDS             1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHUU3_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR2083650.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="ACHUU3_003677"
     CDS             1257..1376
                     /locus_tag="ACHUU3_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR2083651.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHUU3_003678"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHUU3_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR2083652.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHUU3_003679"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHUU3_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR2083653.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHUU3_003680"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHUU3_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR2083654.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="ACHUU3_003681"
     CDS             4176..4544
                     /locus_tag="ACHUU3_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR2083655.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHUU3_003682"
     CDS             complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHUU3_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR2083656.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="ACHUU3_003683"
     CDS             5295..5588
                     /locus_tag="ACHUU3_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR2083657.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="ACHUU3_003684"
     CDS             5660..6319
                     /locus_tag="ACHUU3_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR2083658.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="ACHUU3_003685"
     CDS             6411..6857
                     /locus_tag="ACHUU3_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR2083659.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..7966)
                     /gene="hlyE"
                     /locus_tag="ACHUU3_003686"
     CDS             complement(7064..7966)
                     /gene="hlyE"
                     /locus_tag="ACHUU3_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFR2083660.1"
                     /translation="MVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKEL
                     SRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYN
                     EKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKS
                     SYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQSF
                     FTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAK
                     KMINTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         2049 a         1789 c         1836 g         2293 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcacctt ggttagaggc
     2281 gcgcaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ccccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     7501 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt
     7561 gtgagcttac cagcagggat ttttgcgctt cattcagctt cgtgatgccg tcatccagta
     7621 ccttaatgag aatgtctttc tgggcggatg ctttcttctc attgtactca tcaaatagca
     7681 aaatatacgc agcgagcaat tgcgtcgcaa caccacacca ttcatacact gtttgggttg
     7741 cttcaaaata cttatcctgg ctatccataa gtaaggtttt aatatcgccg actaaaacgg
     7801 aggctgcctg tgaatactcc tgtttaaagc gacttaactc ttttatggtt tcatcaaagg
     7861 tctgccaggg gatgacctga tcgagatatt tattataaag atctaatgct ccatctgcgg
     7921 tttcgattgc gtttttaact acttctaccg ttttatctgc aacgatt
//
LOCUS       DAZPLI010000176         7951 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZPLI010000176 DAZPLI010000000
VERSION     DAZPLI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7951
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.177"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..365
                     /locus_tag="ACHUU3_003687"
     CDS             <1..365
                     /locus_tag="ACHUU3_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HFR2083661.1"
                     /translation="ITGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERL
                     GERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFL
                     LRKDIMRRLTQDVCEEIE"
     gene            complement(422..2800)
                     /gene="ppsA"
                     /locus_tag="ACHUU3_003688"
     CDS             complement(422..2800)
                     /gene="ppsA"
                     /locus_tag="ACHUU3_003688"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR2083662.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            3133..3966
                     /gene="ppsR"
                     /locus_tag="ACHUU3_003689"
     CDS             3133..3966
                     /gene="ppsR"
                     /locus_tag="ACHUU3_003689"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR2083663.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4123..5169
                     /gene="aroH"
                     /locus_tag="ACHUU3_003690"
     CDS             4123..5169
                     /gene="aroH"
                     /locus_tag="ACHUU3_003690"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR2083664.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            5301..5492
                     /gene="hemP"
                     /locus_tag="ACHUU3_003691"
     CDS             5301..5492
                     /gene="hemP"
                     /locus_tag="ACHUU3_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR2083665.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5496..6932)
                     /gene="selO"
                     /locus_tag="ACHUU3_003692"
     CDS             complement(5496..6932)
                     /gene="selO"
                     /locus_tag="ACHUU3_003692"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR2083666.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(6995..7708)
                     /gene="ydiV"
                     /locus_tag="ACHUU3_003693"
     CDS             complement(6995..7708)
                     /gene="ydiV"
                     /locus_tag="ACHUU3_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR2083667.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         2034 a         2086 c         1888 g         1943 t
ORIGIN      
        1 aaattaccgg acgcaaaaaa gatattattg tccgcggcgg cgaaaatatt agcagccgtg
       61 aagtagaaga tattttattg cagcatccta aaattcacga tgcctgtgtg gttgcaatgc
      121 ccgatgaacg tttaggtgaa cgatcatgcg cttatgtcgt gctgaaagcg ccgcatcatt
      181 cattatcgct ggaagaggtg gtggcttttt ttagccgtaa acgggtcgca aaatataaat
      241 atcctgaaca tatcgtggta atcgaaaaac taccgcgtac tgcctcaggt aaaatacaaa
      301 agtttttgtt aagaaaagat attatgcggc ggttaactca ggatgtctgt gaagagattg
      361 aataagtttc atcttcgggg atcacataac cccggcgact gaacgccgcc ggggatttat
      421 tttatttctt cagttcagcc aagcttaacc aggtttgcac cacggtgtcc gggttcagag
      481 acaggctatc gatcccctct tccatcaacc atgcggcaaa gtcttcgtgg tcggacggac
      541 cctgaccgca aatcccgaca tatttgccct gtttcttcgc ggcacggata gccatcgaca
      601 gcagtgcttt caccgcatcg ttgcgctcat cgaacaattc agacaccacg ccggagtcac
      661 ggtccagacc gagcgccagc tgcgtcatat cgtttgagcc aattgagaag ccgtcgaaat
      721 attcgaggaa ctgctcggcc agcaaggcgt tggacgggat ttcacacatc atgatgattt
      781 tcagcccgtt ctcaccacgc ttcagcccct gacgcgccag ttcctcaacc accgctttcg
      841 cctgatctac ggtacgcacg aacgggatca tgatctcaac gttggtcagc cccatgtcgt
      901 tgcgcacacg tttcactgct tcgcactcca gcgcgaagca gtcgcggaag ctgtcggaaa
      961 catagcgtcc cgcgccacgg aagccgagca tcgggttctc ttcatctggc tcgtaacgct
     1021 caccaccgac caggttggca tattcgttcg atttaaaatc agagagacgg acaatgacgc
     1081 gcttcggata aaacgcggca cccagcgtcg cgatcccttc agtcagacga ccaacgtaaa
     1141 attcacgcgg agaatcaaaa cctttcatca tctcgcggat ttcattttgc aactgcggtt
     1201 cctgatcgtc aaactcaagc agtgcgcgtg ggtggacgcc aatcatacgg ttgatgataa
     1261 attccagacg cgcaaggccc acgccttcgt tcggcaggca ggcgaagtcg aaagcacggt
     1321 ccgggttacc gacgttcatc atcactttca acggcagatc cggcatcatt tctacgctgg
     1381 agcttttcac gctaaattcc agcaactccg catagacgta accggtatca ccttcggcac
     1441 aagaaacagt gacgttctca ccgtctttca tccgttctgt tgcatcacca cagcccacta
     1501 ccgccggaat gcccagttca cgagcgatga tcgccgcgtg acaggtacga ccgccacggt
     1561 tggtgacgat ggcagatgct ttcttcatga tcggttccca gtccgggtcg gtcatgtcag
     1621 tgaccagcac gtcaccaggt tcgatgcggt tcatttcgct gatctcatgg atgactttca
     1681 ccggacccgc gccgatgcga tgaccgatag cacggccttc ggcgataatc ttaccctgtg
     1741 aatgcagcgt ataacgctcc atgacctgac cgcgtgagcg cacggtttcc ggacgcgcct
     1801 gcacaatgaa cagtttgcca gtgtggccat ctttcgccca ctcaatatcc atcgggcgac
     1861 cgtagtgttt ctcaatttgt acggcctgtt ttgccagttc ctgcacttct tcgttggtca
     1921 gcgagaagat gtcacgctgt tcctgcggta cgtcttcgat tttaacctgc ttgccgtgct
     1981 cctgggtcgg cgcgtaaacc atgcggattt ttttcgaccc catggtgcga cgcacgatag
     2041 ccgggcgatt cgccgccagt gtcggtttat ggacgtaaaa ctcatccggg ttaaccgcac
     2101 cctgcacgac catttcccca aggccccatg cggaagtgat aaacaccacc tggtcaaagc
     2161 cggattcggt atcaatggag aacatcacgc cagaagatgc gaggtcggag cgcaccatcc
     2221 gctgaacacc ggcagagagc gccacgccac ggtgatcgta accctggtgc acacgataag
     2281 agatggcgcg atcgttaaac agagaagcaa atacatgctt cactgccacg agaacggcgt
     2341 caaaaccctg aacgttgagg aaggtttcct gctgaccggc aaaagaagcg tccggcatat
     2401 cttctgcggt ggcggaggag cgcaccgcaa aagaggcgtt ttcgtcatcg gcggaaagct
     2461 gtgcataggc ttcgcggatg gcgttttcca gctcaggctg gaagggagtg tcgataatcc
     2521 actggcggat ttgcgcgccc gctttcgcaa gctgagtaac atcgtcaata tccgttttat
     2581 ccagcagttc ataaatgcgc tggtttacgc cgctttggtc cagaaactgg ttaaacgcgt
     2641 cggcggttgt ggcgaaacca ttcggaacgg aaacacccat tccggaaagg ttagtaatca
     2701 tttcacccag ggaggcattt ttgcccccaa ccctgtctac atcattcatg ccgagttggt
     2761 tataccaaag caccagcggt gacgagccat tgttggacat cgaacaatcc ttttgtgata
     2821 aatgaacggt ttgagaaaca catttctgcg catttatctt tgcatattta accggatgaa
     2881 aaaaacggtg aatcgttcaa gcaaatatat ttttttactt tttaagactg atcccagcgt
     2941 tgcgcaaatc tgctctcttc gacaatttcc acataaacca tcggtataaa cgaaaccata
     3001 aaaatgaaat gctgttttca taaaaaataa aattgaaggt tcattttata aaccagacat
     3061 aacgtcctac gcttctgtgc gtttttaatt tatgctttca tagaattatg tctgcatcac
     3121 gggaagaaca aaatggataa tgctgttgat cgccacgttt tttatatttc tgatggtacg
     3181 gcaataactg cggaggtatt aggacacgca gtaatgtcac aatttcccgt cactatcagc
     3241 agcattacgc tgccgtttgt cgaaaatgag agccgtgcac gggcagtgaa ggatcagatt
     3301 gacgcaattt atcaccagac aggtgtgcgc ccgctggtct tctactccat cgtgttgccg
     3361 gagattcgcg ccatcatctt gcaaagtgaa ggcttttgcc aggatatcgt tcaggcgctg
     3421 gttgccccgc tacaacaaga gatgaaactg gatccaacgc cgattgctca tcgtacccat
     3481 ggccttaacc ctaataatct caataaatat gatgcgcgca ttgcggcgat tgattacacc
     3541 ctcgcccacg atgacggcat ttcgttgcgc aatctggatc aggctcaggt gatcctgctc
     3601 ggtgtttctc gctgtggtaa aacccccacc agtctttatc tggcaatgca atttggtatc
     3661 cgcgcggcaa actacccctt tattgccgac gatatggata atctggtgct acccgcgtcg
     3721 ctcaaaccgc ttcagcataa attgttcggc ctgactatcg acccggaacg tctggcggcg
     3781 attcgcgagg aacgtcggga gaacagtcgc tatgcctcgc ttcgtcagtg caggatggaa
     3841 gtcgcggaag tggaagcgtt gtaccgtaaa aatcagatcc cgtggattaa cagtaccaat
     3901 tattcggtag aagagattgc caccaagatc ctcgatatca tgggccttag tcgccgaatg
     3961 tactagagaa ctagtgcatt agcttatttt tttgttatga tgctaaccac ccggcgaggt
     4021 gtgacacacc tcgcacttga aatcagcagc gattggttta tcgtgatgcg catcacttcc
     4081 cggcagtcct gccgtagaag caacaaattt ctgagacttg taatgaacag aactgacgaa
     4141 ctccgtactg cgcgtattga gagcctggta acgcccgccg aactcgcgct acggtatccc
     4201 gtaacgcctg gcgccgccac ccatgtcacc gactcccgcc gcagaattga aaaaatactc
     4261 aatggtgaag ataagcgact gttggtcatt attggcccct gctcgatcca cgatctcacc
     4321 gctgcaatgg agtacgcccc ccgtctgcag tcgctgcgca accagtacca gtcacggctg
     4381 gaaatcgtaa tgcgcaccta ttttgaaaaa ccacgaactg ttgtcggctg gaaaggacta
     4441 atctccgatc cagatttaaa cggcagctat cgggtaaatc acggtctgga gctggcgcgc
     4501 aaattacttt tacaggtaaa tgagctgggc gtcccaaccg cgactgagtt cctcgatatg
     4561 gtgaccggtc agtttattgc tgatttaatc agttggggcg cgattggcgc acgtactacc
     4621 gaaagtcaga tccaccgtga aatggcttcg gcactctcct gtccggtagg ttttaaaaat
     4681 ggtaccgatg gcaatacgcg gattgctgtg gatgctatcc gcgcagcccg cgccagccat
     4741 atgttcctct cgccagacaa aaatggtcag atgaccatct atcagaccag cggcaacccg
     4801 tatggccaca ttattatgcg tggcggcaaa aaaccgaatt atcatgccga tgatatcgcc
     4861 gcagcctgcg atacgctgca cgagtttgat ttacctgaac atctggtggt ggatttcagc
     4921 cacggtaact gccagaagca gcaccgtcgc cagttagaag tttgtgagga tatttgtcag
     4981 caaatccgca atggctctac ggcgattgct ggaattatgg cggaaagttt cctgctcgaa
     5041 ggaacgcaaa aaatcgtcgg cagtcagccg ctcacttacg gtcaatccat taccgacccg
     5101 tgtctgggct gggaagatac cgaacgcctg gtcgaaaaac tcgcctctgc ggtagatacc
     5161 cgcttctgaa tgcgtgccca ttcctgacgg aatgggcatt tctgcgcaac ttgttgtctt
     5221 ctcaacaaat tactgcttgc tctggcctgc cataatattg ataataagaa tcattgttat
     5281 atcaattatt attaatcttt atgcgttata cggatagcag aaaactcacg cctgaaacgg
     5341 acgccaatca caaggccgct tccccgcagc ccatccggcg aattaccagc cagacactgt
     5401 taggtccgga tggcaaactg attatcgatc atgacgggca agaatatctg ctccgtaaaa
     5461 ctcaggctgg caagctgctg ttaactaagt agcctttaac tcgagcagct gacttccagc
     5521 cgtttacccc agtcaggtgg acgactgaca tagtcatcat ctctatcgct gaaaggattt
     5581 cgcaacgccc catgcaggcg gtgcaattcc gtcatatcac ccttttctgc cgcttcaatc
     5641 gcccgttgcg ccaaccagtt gcgcaatacc agagcggggt taacgctttg catcaactgc
     5701 tgacgctcac tatcgctaac ctcgtcttgt tgcaaacgcc cccgataacg ggcaaaccag
     5761 ccatcaaatg ccgcacgatc aataaactca tcacgtagcg gtgacgccgt gctgtgctgc
     5821 tcggtcagac tcagcatgcg gaatgtgcgg gtataatcgc tgcgctctcg cgccatcaga
     5881 ctgaataatt cattcagtag cgcgttatcc tctttttgct ccgtgatgaa gcccagtttc
     5941 tgccgcatcc gttgtccata atgcgtcaac aaaacctgct gatagctgtc cagggcctca
     6001 ttcagggcat ctacggcaac aaatggtgac aatgtctgcg ccagacgctg taaattccac
     6061 aacgcgacag caggttgatt atcaaaacta taacgccctt gatgatccga gtgattacaa
     6121 ataaaaccgg gttcgtaatc atcaagaaaa ccaaacggcc cgtaatcaag cgtcagtccc
     6181 agcagcgaca tgttgtcggt attcatcacc ccatgtgcaa agccgaccgt ctgccattgg
     6241 gcaattaacg acgcggtacg tgcgacaaca tcgctaaacc agagacggta tttgtcctca
     6301 tcatcttcaa gatgtgacca gtaatgacga atagcaaagt cagctaactg acgaaccttt
     6361 tccgactcgc ggcggtagta aaaatgttcg aaatgaccaa agcgcagatg acttggtgcc
     6421 acacgcatca gcatcgcgcc tggctccgcc gtttcccgat acactggcga atcgctggtg
     6481 acgatactta acgcgcgggt cgtcggaatg cccagataat gcatcgcctc actggcgaga
     6541 ctttctcgta tcgtcgaacg taaaaccgcc cgtccatcac ccattcgcga ataaggcgtc
     6601 aggccagcac ctttcagatg ccagtccatt gtagtgccat cagcaagcag ttgttcgccg
     6661 agtaaaatgc cgcgcccatc acccagttgg cccgcccaga cgccgaactg atgaccactg
     6721 taaacctgcg ccagtggtga cataccaggc agtagggctt cgccgcccca gacacctgcg
     6781 ccatttttaa acagcgacga tggaatactc agcgtgttag ccagttcggt attatgccaa
     6841 atcagccggg cattatttaa aggcgtaggg gaaagtgctg tataggtttc tggcaattca
     6901 tcgcgccagc gggtaacaaa agacagggtc atagatcctc ctgtttgata gtgtagacgg
     6961 ttactctcgt cttaaacacc agcaaacaga agggttatcg ctgaaccaac gtcgttatct
     7021 gatttatggg aacggcaggc cacaggcatc cctggagagc atggaaatcg aagggggtaa
     7081 tctgtgccaa tatttctgcc gtatcaatgc ctcccgcaat gatgcaattg caacaagggg
     7141 aaatttgcgc ctggatcgca cgaataaacg gttcgaagga gcggtgcgtg atttgttgct
     7201 gaataaaact tttatccagc atgacgcggg tgaaaagacc atcaaaaacc gctttcattg
     7261 tgctattgcc tgctcccaaa ttacctaaca caaggggata cacttgcgat aaggataaaa
     7321 gaccgcgatt atcttttcct tcattgagat gcgggtaatt ctcattaatc aacagttcaa
     7381 taaacggata ttttaatagt tcgcctgcgt aattatccct ctctaataat aatgtagcca
     7441 cttgtggcgt taaattaagc caggcaaaga gtttatgttg aataaaaaaa tgttggcatg
     7501 attttagtaa ttccaactgc tctgaaaata attgccaatg ttgctcctcg gtaagttgcg
     7561 ctataacccg actcgtcggg atacgcaccg tgccatcttc actggagaaa tgggtaatta
     7621 actcaacgcc gacaaggact tgctggttat ctcttatcgg caggaaatag caatcagaat
     7681 gataaagatt ctccaaaaaa atcttcatgg taaccgtccc tctcgaagga tgtttttcag
     7741 tatccggcta aaagggatga ggccataaga cataacaatt acagaaggag taactttcat
     7801 ttgttccatg ttaaccactt tttcaggggt ccttttttta gattatcctg attataaacg
     7861 aataatccat ttacggaatt tttgtctgca aaatactact gtattcagga gtaaatggac
     7921 ggcgatgtat catcgccgtc catttttcga c
//
LOCUS       DAZPLI010000177         7774 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZPLI010000177 DAZPLI010000000
VERSION     DAZPLI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.178"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..1780)
                     /locus_tag="ACHUU3_003694"
     CDS             complement(<1..1780)
                     /locus_tag="ACHUU3_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HFR2083668.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPERLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHUU3_003695"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHUU3_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR2083669.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHUU3_003696"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHUU3_003696"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR2083670.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHUU3_003697"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHUU3_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR2083671.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHUU3_003698"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHUU3_003698"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR2083672.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="ACHUU3_003699"
     CDS             complement(5968..7005)
                     /locus_tag="ACHUU3_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR2083673.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7339..>7774
                     /gene="yhhY"
                     /locus_tag="ACHUU3_003700"
     CDS             7339..>7774
                     /gene="yhhY"
                     /locus_tag="ACHUU3_003700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR2083674.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKY"
BASE COUNT         1976 a         2102 c         1872 g         1824 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc gttcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ctgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt acatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaaa gccagcaccc
     7141 ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg ttctccgcga
     7201 gggtcttcct gatcgcgaga caataataat cattctcatt cgcacttgtc caacactttt
     7261 tgcaaaaaaa atgcatttga ctcgcatttg aaagtcaatg atgttgaaag ggacatttac
     7321 cccaaagagg acaaaggaat gagtgagata gtaatacgcc acgcagaaac acgggattac
     7381 gaggccatca ggcagattca cgcccagccg gaggtgtatt gcaacacact acaggtgcct
     7441 catccttccg atcatatgtg gcaggagcga ctcgccgatc gtcccggcat caagcaactc
     7501 gtcgcctgta ttgatggaga cgtcgtgggc catctcacca ttgacgtgca acagcaccca
     7561 cgccgcagtc atgttgccga ttttggtatc tgtgtcgact ctcgttggaa gaaccgcggc
     7621 gtcgccagcg ccctgatgcg agagatgatt gaaatgtgcg acaactggtt gcgggtagat
     7681 cgcattgaac taaccgtgtt tgtcgataac gccccggcaa ttaaggtcta taaaaaatac
     7741 ggctttgaaa ttgaagggac tggtaagaag tacg
//
LOCUS       DAZPLI010000178         7611 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZPLI010000178 DAZPLI010000000
VERSION     DAZPLI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.179"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            171..701
                     /locus_tag="ACHUU3_003701"
     CDS             171..701
                     /locus_tag="ACHUU3_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR2083675.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGDWFNDGNPTWDDGDGEDLYLKEV
                     THWMQLPEPPQEVNRG"
     gene            837..1502
                     /locus_tag="ACHUU3_003702"
     CDS             837..1502
                     /locus_tag="ACHUU3_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083676.1"
                     /translation="MLLKMVNEARKLYGEPSVRNNKFIEKIEDELEGETYTKSVGRKN
                     GADIDVISMTIKQALRVAARESKAVRRTLVDKLESMQEAHIKSGKSASGLVEYRQART
                     LKMTVEAVTNLFDLMPNLAPEAKQTAAASIINPLVGFNAIPLPAIEEHYYSAGEVAEQ
                     LGVTANKIGRIANANNLKTEQYGKFFLDKSAHSSKQVETFRYNEAGVHKIEELIEGER
                     KAA"
     gene            1824..2447
                     /locus_tag="ACHUU3_003703"
     CDS             1824..2447
                     /locus_tag="ACHUU3_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFR2083677.1"
                     /translation="MASQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            2532..2750
                     /locus_tag="ACHUU3_003704"
     CDS             2532..2750
                     /locus_tag="ACHUU3_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR2083678.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            2728..3798
                     /locus_tag="ACHUU3_003705"
     CDS             2728..3798
                     /locus_tag="ACHUU3_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR2083679.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTKPVAATRAAKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            3933..5216
                     /locus_tag="ACHUU3_003706"
     CDS             3933..5216
                     /locus_tag="ACHUU3_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR2083680.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(5450..>7611)
                     /locus_tag="ACHUU3_003707"
     CDS             complement(5450..>7611)
                     /locus_tag="ACHUU3_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR2083681.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSS
                     VWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYE
                     IDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENV
                     IAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFG
                     AGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASE
                     LDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVC
                     SKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAG
                     NVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLAN
                     IAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIH
                     EDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
BASE COUNT         2093 a         1863 c         1896 g         1759 t
ORIGIN      
        1 cgctacgcga taagcaggcg ttacttgaag cctcagaaaa gcgcatagca gaactggagg
       61 cacagacagt taccgttaaa gaggttggag atgcttgata gttttgcgct cgttgcactg
      121 gttggcattg tagcgttgat ttgggtgaca tccgggaatc ggaggcataa gtgaacgaca
      181 aagaactgat taaagaaatc aaagagcgta tcagcagttt ggatgtgcga gacaatgttg
      241 aacgccgtgc ttatgaaatt gctctggcat cgctgacagc agagccggtg gcgtggaagg
      301 taaccttcac gcaaattgac cgtgaatata acacgttcac tggtatgtat tctgacaaag
      361 cagaagtcga acggtgggtg cggctgcata aagcatgtaa ttttcgggca gatataacac
      421 cgctttatac cgcccagcca gtgccggtaa ctccggatgc ctggataagc tgtagtgagc
      481 gaatgccaga aatgggagag cgacaatgct atgtgttagc agctgacttt aaaaacaact
      541 acccaccaaa catccccaac actcaggtcg gcgtatatgg cgactggttt aatgatggca
      601 atcccacttg ggatgacggt gatggcgaag acctgtatct caaagaggta acccactgga
      661 tgcagctacc agaaccgccg caggaggtta accgtggcta acctgcaact tgccgtcaaa
      721 ggtgaataac aatcctcgca ctcgcgggga tttcttttat acgatatagg ggaaaaggtg
      781 tggttaacaa tattgcaaat cactcttcgt ttccggctgt aacaatcgac agccagatgc
      841 tgttgaaaat ggtcaatgaa gctcgcaagt tatatggaga gccatcagta cgtaacaaca
      901 aattcatcga gaaaattgag gatgaattgg aaggcgagac ctacacaaaa agtgtaggtc
      961 ggaaaaacgg ggctgacatt gatgttatct ccatgactat caagcaggcg cttcgtgttg
     1021 ctgctcgcga atctaaagca gttcgccgaa cacttgtaga caaacttgaa agtatgcagg
     1081 aagcgcacat taaaagcggt aaatcagcga gtggacttgt tgagtatcgt caagcgcgaa
     1141 cactgaaaat gacggttgaa gctgttacca atctgttcga tttgatgcca aatcttgcgc
     1201 cggaagcaaa gcagactgct gcggcaagca taatcaaccc gctcgttggt tttaatgcaa
     1261 tacctcttcc ggcaatagaa gagcattact actcagcagg ggaggtcgca gagcagctcg
     1321 gagtaacggc aaacaagatt ggtcgcattg ctaacgcaaa caacctcaaa actgagcagt
     1381 acgggaagtt cttcctggat aaatctgcgc attccagcaa gcaggttgaa acatttcggt
     1441 acaacgaggc tggagtgcac aaaatagagg aactcatcga aggagagcga aaggctgcat
     1501 gattttgaca agatagtttt ccccaaatgt ggggaaaagc ccgaatggcg cggcttacag
     1561 caagataagg cctacatgat ttgacaacac cgcattaacg gggctatatt ccgcttcatg
     1621 gtgctgaaca caccttgcaa agcggaaacc gcacccgtca gtcatgcgga ttttttatgt
     1681 ccatttttca gatatggtcg ggtagcgcgt ataccgaaaa acagccgaaa ggttaaggat
     1741 acgggccgac tttgcacggt gttcaagtac ctgaccgccc tgctgaacac agggctatct
     1801 gaacaaatgc aaaggacata aaaatggcca gtcaactcat ccccgtattc aacggcacta
     1861 tatccaacga aacaactctt ctcgttaatg cccgtgattt acacgaattt ttggaagtac
     1921 gtcgtgactt ctctacatgg attaaaaatc gcataacaga atacggcttt gccgttgatg
     1981 tcgattacat tttggttcac caaaacaggg ggatcaaagg acgtggaggc gatcgccgta
     2041 gcaaagacta tcacctcacc ctcgatacag ccaaagaaac ggcgatggtc gagcgtaacg
     2101 aaaaaggccg ccagatacgc cgatacttca tcgagtgcga aaagaaactt cgcagcatgc
     2161 aaccagcgca gcaattcaca gacgaagaaa tcatcctcct ttgctatatg caggtacaga
     2221 tggagaatgc gcaggacatc tgcaaacgtc tgtacccgat attgaaggaa ctgaactcat
     2281 catacacgag caagctgtat gacatcgcgt ttgaaacttt ttacacggtg acgaaaaaca
     2341 gagatgtact gctaagggag gcgacacgac ttgaccaagc aagcgccctt ttcgaacggg
     2401 caaaaccaat gctgaaaagc cttcgggcga gacaattcga attttaatca tcaaaggagc
     2461 ttcggctcct tttttgttgg agaaaataaa ccaatactcg ctcccttgcg agtaattgcg
     2521 gagactttgc gatgtacttg acacttcagg agtggaacgc acgccagcga cgcccaagga
     2581 gccttgaaac agttcgtcga tgggtacgcg agtgcaggat attccctcct ccggttaagg
     2641 atggaagaga gtatttgttc cacgaatcag cggtaaaggt tgacttaaat cgaccagtaa
     2701 caggtagcct tttgaagagg atcagaaatg ggaagaaggc gaagtcatga gcgccgggat
     2761 ttacccccta acctttatat aagaaacaat ggatattact gctacaggga cccaaggacg
     2821 ggtaaagagt ttggattagg ccgagacagg cgaatcgcaa tcactgaagc tatacaggcc
     2881 aacattgagt tattttcagg acacaaacac aagcctctga cagcgagaat caacagtgat
     2941 aattctgtta cgttacattc atggcttgat cgctacgaaa aaatcctcgc cagcagagga
     3001 atcaagcaga agacactcat aaattacatg agcaaaatta aagcaataag gagggggctg
     3061 cctgatgctc cacttgaaga catcaccaca aaagaaattg cggcaatgct caatggatac
     3121 atagacgagg gcaaggcggc atcagccaag ttaatcagat caacactgag cgatgcattc
     3181 cgagaggcaa tagctgaagg ccatataaca acaaaaccgg tcgctgccac tcgcgcagca
     3241 aaatcagagg taaggagatc aagacttacg gctgacgaat acctgaaaat ttatcaaaca
     3301 gcagaatcat caccatgttg gctcagactt gcaatggaac tggctgttgt taccgggcaa
     3361 cgagttggtg atttatgcga aatgaagtgg tctgatatcg tagatggata tctttatgtc
     3421 gagcaaagca aaacaggcgt aaaaattgcc atccctacaa cattgcatgt tgatgctctc
     3481 gggatatcaa tgaaggaaac acttgataaa tgcaaagaga ttcttggcgg agaaaccata
     3541 attgcatcta ctcgtcgtga accgctttca tccggcacag tatcaaggta ttttatgcgc
     3601 gcacgaaaag catcaggtct ttccttcgaa ggggatccac ctacctttca cgagttgcgc
     3661 agtttgtctg caagactcta tgagaagcag ataagcgata agtttgctca acatcttctc
     3721 gggcacaagt cggacaccat ggcatcacag tatcgtgatg acagaggcag ggagtgggac
     3781 aaaattgaaa tcaaataatg attttatttt gactgataat gacctgttcg ttgcaacaaa
     3841 ttgataagcg atgctttttt ataatgccaa cttagtataa aaaagcaggc ttcaacggat
     3901 tcatttttct atttcatagc ccggagcaac ctgtgaacac attttcagtt tcccgtctgg
     3961 cgctggcatt ggcttttggc gtgacgctga ccgcctgtag ctcaaccccg cccgatcaac
     4021 gtccttctga tcaaaccgcg cctggtacct cttctcgccc gattctgtcg gcaaaagaag
     4081 cgcagaattt cgatgctcaa cactattttg catccctgac accaggtgct gcagcgtgga
     4141 atccttcccc gattaccctg cctgcgcaac ctgactttgt tgtcggcccg gcgggcactc
     4201 aaggtgtaac gcataccacg attcaggcgg cggtagatgc ggcaattatc aagcgtacca
     4261 acaagcgcca gtatattgcc gtgatgcctg gtgagtatca gggaacggta tatgtccctg
     4321 ccgctccggg tggaattact ctgtacggta caggtgaaaa accgattgat gtgaagattg
     4381 ggctttccct tgatggtggc atgagccctg ccgactggcg tcacgacgtc aacccgcgcg
     4441 gcaaatatat gccaggtaaa ccagcgtggt atatgtacga tagctgccag agcaaacgca
     4501 gcgacagtat cggtgttctc tgctctgcgg tcttctggtc acaaaacaat ggcctgcaac
     4561 tgcaaaatct gaccatcgaa aacacgctgg gcgatagcgt agatgcaggt aaccatccgg
     4621 cggtggcact gcgtactgat ggtgaccagg tacagattaa caacgttaac attctcggtc
     4681 gtcagaacac cttctttgtc accaacagcg gtgtgcagaa ccgtctggaa acgaatcgtc
     4741 agccgcgtac gctggtgacc aacagctaca ttgaagggga tgtggatatc gtttctggtc
     4801 gcggcgcagt ggtgttcgat aacaccgaat tccgcgtggt gaactcacgt actcagcaag
     4861 aagcgtatgt gtttgcaccg gctacgctgt ccaacatgta ctacggtttc ctcgccgtaa
     4921 acagccgttt caatgctttc ggtgatggtg tggcgcaact gggccgctcg ctggatgttg
     4981 atgccaatac caacggtcag gtggtgatcc gtgatagcgc catcaacgaa ggttttaaca
     5041 cggctaaacc gtgggccgat gcggtgatct ctaatcgtcc gtttgcgggt aataccggca
     5101 gcgtagatga taacgacgaa atacagcgca atctgaatga cactaactac aaccgcatgt
     5161 gggaatacaa taaccgcggc gtgggtagta aagtggttgc agaggcgaag aagtaagagc
     5221 aattaactat ttgccggatg cggcgtaaac gccttatccg gcctacggtt cgatgcgatt
     5281 tgtaggtcgg ataagatgcg caagcatcgc atccgacaat aagtgccgga tgctgcgtaa
     5341 atgccttatc tggcctacag attcgatgcg attcgtaggt cggataagat gcgcaagcat
     5401 cgcatccgac aataagtgcc gaatgcgacc tacattcaca tggcgctttt tacatctgac
     5461 ggtttttatt gaagttaatc aaactacccg ccttgataat ctcgcgctct tcagcagtca
     5521 gactttccat atagagcgta atttccgtta ccggcgcatc ttcatggatc acataacctt
     5581 taaacgtcgt acccggatta tccagcgccg ctttaatgcc agggatgtaa atgtaatccc
     5641 ccacttcaaa ggttggcact tccgccatct gcagaggtaa catcccccag ttgatgacgt
     5701 tagaacggta gcgtttagtc gcgtactcct cggcaatatt cgccagaccg ccaatcacac
     5761 gctggcagct cgccgcctgt tcacgcgcag aaccatcgcc tggtttcacc gcatagacca
     5821 tgctgccaat ttcagtttgc agcggatcaa tatgctcctg accagcaatc tgcttaatgc
     5881 gcgcaaacac ctctgtcagc tcgctgacat tccccgccag acgctgattt tccagctcag
     5941 cagtcgcttt acttctgcca acataaccgg gatcacggcg agacagggta aactccgcca
     6001 gaccaatcgg atttgaacga taagaagagg tttcaccgga aggaatcagt tcgtcggtgg
     6061 tggtcacttc gtcgaggatc ttcgagcaca ctttcaggac gatattgtca gtcagcgcac
     6121 ccaattccgg ccagtcttta atgttcggcc cgtaaatcag cggttgctga gttgcccctt
     6181 tcacaaagcc ctgataaaca cggtttttat acggcgttac atcgaaggcg tactccggca
     6241 cgttgtccca gcaatcaagt tcgctggcag aggttaaata gccaccgttt gccgcagtcg
     6301 cagcgataga acgagcgtcc atcaacgcca ccgctgacat ctgcccatta gctggcttag
     6361 agccttcgcg gttcgggaag ttacgcgtgg tgtggcgaat actcaaaccg ttgttgattg
     6421 gcgtatcgcc cgcgccaaag catgggccgc agaacgcggt tctgatgatt gcgcctgcgc
     6481 caatcaaatc tgctaccaca ccttttttgg cgagatccat aaacaccggc tgtgatgacg
     6541 ggtaaactgc cagcgagaag gtgtcattgc cacaggattg accgcgcagt gcattcgccg
     6601 ccgcgatgac gttttcgtag ttaccgccag aacagcccgc gataatcccc tgctgcactt
     6661 tcaggcgacc attttccact ttatccagca gcgagagttt ggctttaccg tgcgccacgc
     6721 gttcggactc aatttcaatc tcacgcagaa tgtcggtcag gttctggttc agtgtgtcga
     6781 tttcatacac gttgctcggg tggaacggca gcgcaatcat tggtttgatg gcgcttaaat
     6841 caacgctgat gcagccatcg tagtacgcca tcggttgagg gttaagctgg cagtaatcct
     6901 ggccgcgacc gtgcagcgcc agccagttat gcacttcttc atcagtttgc cagacggagc
     6961 ttaaacaggt ggtttccgtg gtcatcacgt caacgctgtt acggaaatcg gtagagagcg
     7021 cgctaacgcc cggtccaacg aactccatga ctttgttttt gacgtaaccg tttttgaaca
     7081 ccgcgccaat gatagccagc gccacatcct gtggccccac atacggcgca ggttttccgg
     7141 tcagatgcac cgcaaccacg cccggatagt cgatatccca ggtgtcatta agcagctgtt
     7201 ttaccaactc accgccaccc tcaccgactg ccattgtccc taatgcaccg taacgggtgt
     7261 ggctgtctga cccgaggatc attttgcccc cgcctgccat catctcacgc atatattgat
     7321 ggatgaccgc aatatgcgga ggcacaaaaa taccgccata acgctgagct gccgataaac
     7381 caaaaacatg gtcatcaccg ttaatggtgc cgccgacggc gcagagtgag ttatggcagt
     7441 tggtcagcac atacggcagc gggaaacgtt ccattccgga cgctttagcg gtctgtacaa
     7501 taccaacaaa ggtaatatcg tgagaggcta atgaatcaaa cttaatttta agtttatcca
     7561 tatttccgga cgtattatga gaagagagaa tagaccaggc aatagtgcct t
//
LOCUS       DAZPLI010000179         7491 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZPLI010000179 DAZPLI010000000
VERSION     DAZPLI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.180"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(16..522)
                     /gene="mug"
                     /locus_tag="ACHUU3_003708"
     CDS             complement(16..522)
                     /gene="mug"
                     /locus_tag="ACHUU3_003708"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFR2083682.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            647..722
                     /locus_tag="ACHUU3_003709"
     tRNA            647..722
                     /locus_tag="ACHUU3_003709"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:680..682,aa:Ile,seq:cat)
     gene            complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="ACHUU3_003710"
                     /gene_synonym="yqjH"
     CDS             complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="ACHUU3_003710"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFR2083683.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1828..2451
                     /gene="nfeR"
                     /locus_tag="ACHUU3_003711"
                     /gene_synonym="yqjI"
     CDS             1828..2451
                     /gene="nfeR"
                     /locus_tag="ACHUU3_003711"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR2083684.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(2558..4078)
                     /gene="aer"
                     /locus_tag="ACHUU3_003712"
     CDS             complement(2558..4078)
                     /gene="aer"
                     /locus_tag="ACHUU3_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFR2083685.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4496..5875
                     /gene="ygjG"
                     /locus_tag="ACHUU3_003713"
     CDS             4496..5875
                     /gene="ygjG"
                     /locus_tag="ACHUU3_003713"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR2083686.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5917..6249)
                     /locus_tag="ACHUU3_003714"
     CDS             complement(5917..6249)
                     /locus_tag="ACHUU3_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFR2083687.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6468..7451
                     /gene="ebgR"
                     /locus_tag="ACHUU3_003715"
     CDS             6468..7451
                     /gene="ebgR"
                     /locus_tag="ACHUU3_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR2083688.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1823 a         2031 c         1869 g         1768 t
ORIGIN      
        1 tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct cgcgatacgc
       61 ttcaaccagt ttctctagtg aaacgcgact taaaccgctg ggatttggca gcacccaaat
      121 ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgcgct ggctgaatcc
      181 ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat cttcaatttt
      241 ttcaatcagc ttacgcccac ctgcgtgcag ctcctgcttt gaaacttcat tggcttgcac
      301 cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat gctgtgcctc
      361 ctgcggcttc aactgacggt cggtaaaccc ggcctgatat atcaccttcc agaagcgatt
      421 tgctggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt tgataccgca
      481 aaacacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc ctttctaaca
      541 tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc aggcgcacgg
      601 taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc ccttagctca
      661 gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac
      721 cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat atcagttact
      781 ttgcgtgcca gtaagccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc
      841 ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg
      901 cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt
      961 cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgcacgctaa
     1021 ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccaag cggcggcgca
     1081 gtgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca
     1141 ccagcgaacc gcgcggacct gcaaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc
     1201 agccgctggc gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt
     1261 cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc
     1321 cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc
     1381 tgtgatcgtc aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgccg aggacaatgc
     1441 gctgaaaacc ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg aagcgcagat
     1501 cattgcgaac gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg
     1561 taatcagata tatctaaata attttcgtaa atgataatga ttgttaatca tgataaatgc
     1621 aagcgatttg tagaactgat atgtctatag tctgataaga cgaaccgcct cttctcaggc
     1681 atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt
     1741 ccacatttaa aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata
     1801 tatctaatca agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat
     1861 gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc
     1921 ggacacggtc accagcatga acacggtcaa tgctgcggtg gtcgccacgg tcgcggcggc
     1981 ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc
     2041 tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg
     2101 aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg
     2161 ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc
     2221 gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg
     2281 cgttgcgttg gtgctgcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt
     2341 aaagcggtgc tggatttacg cgtcaaccag agcgatatca ctgatgcaca aataaaaaag
     2401 atcattgcgg tgatcgacca cgccgctttt gatattaccc aactggatta atcaccgatt
     2461 gccggatgcg gcgtacccta cacaccccgc aggcctgata agatgcgcca gcatcgcatc
     2521 aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc gtcaccgcgt
     2581 cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc tcttccacca
     2641 gctcagcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc aggctggaaa
     2701 gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg gtgacgtttt
     2761 tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc tgcgaaccgg
     2821 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattagcgc
     2881 tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg ccctgttcgc
     2941 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
     3001 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
     3061 tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg gcggtgatag
     3121 aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
     3181 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
     3241 catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc tggcttgaga
     3301 catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
     3361 ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc gccaccttca
     3421 gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa caagcgcttg
     3481 ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca acaaaccaaa
     3541 gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg
     3601 gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta tgaatacgct
     3661 tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca atctcttcat
     3721 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
     3781 ccgcattggc ccgcacccaa taatggtcgc catttttgcg gcgatttttc acgatgccgc
     3841 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
     3901 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta aagccgctca
     3961 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
     4021 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
     4081 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
     4141 tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta aagcacaaat
     4201 ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
     4261 cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat catcaaatcc
     4321 atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat taaatattgc
     4381 acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
     4441 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
     4501 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
     4561 gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
     4621 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
     4681 ttacggagcc gtagagtggc aagcgggaag tttaaatacg cttgtcgaca cccagggaca
     4741 ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt
     4801 tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct
     4861 cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa
     4921 atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa
     4981 agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg
     5041 taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgcc
     5101 gttactgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct
     5161 taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg
     5221 tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta agctatgcga
     5281 tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa
     5341 gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg ccaaagcgct
     5401 cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt tttcagttct
     5461 gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc
     5521 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgatcaaaa
     5581 aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca
     5641 ggaagcgcgt ggtaaaggga tgttgatggc gattgagttt gttgataacg aaatcggcta
     5701 taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc
     5761 caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg aactggtgat
     5821 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca
     5881 aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta cgcccgcagg
     5941 catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt ccgcacacaa
     6001 cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac
     6061 caccgttttt cccatcagct cctcttcgct gtagtacggc accaggctgg tcacggtttg
     6121 cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg
     6181 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
     6241 ggtttccatt gcttcctccc ttcaagtaaa attttactaa actatagcaa aattttttct
     6301 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtaag ccgctgtgtt
     6361 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
     6421 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacattaa
     6481 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
     6541 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
     6601 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
     6661 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
     6721 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
     6781 aacacaacgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
     6841 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
     6901 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
     6961 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
     7021 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
     7081 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
     7141 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
     7201 ttgctatcgg cgtactgcgg gcaattcatg aacgtggcct gaacatccca caggatattt
     7261 cgcttttcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
     7321 tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc
     7381 gcgatggtcg cgcgctgccg ctgctagtct tcgttcccag caaattaaaa ctgcgcggca
     7441 cgacccgtta aatcccctta cacactgtcc ggcaatcatt tttgccggac a
//
LOCUS       DAZPLI010000180         7477 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZPLI010000180 DAZPLI010000000
VERSION     DAZPLI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7477
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.181"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            38..250
                     /locus_tag="ACHUU3_003716"
     CDS             38..250
                     /locus_tag="ACHUU3_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083689.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            283..501
                     /locus_tag="ACHUU3_003717"
     CDS             283..501
                     /locus_tag="ACHUU3_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR2083690.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            572..889
                     /locus_tag="ACHUU3_003718"
                     /pseudo
     CDS             572..889
                     /locus_tag="ACHUU3_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHUU3_003719"
     CDS             complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHUU3_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR2083691.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1800..1889
                     /locus_tag="ACHUU3_003720"
                     /pseudo
     CDS             1800..1889
                     /locus_tag="ACHUU3_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1917..2660
                     /locus_tag="ACHUU3_003721"
     CDS             1917..2660
                     /locus_tag="ACHUU3_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR2083692.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2690..3229
                     /locus_tag="ACHUU3_003722"
     CDS             2690..3229
                     /locus_tag="ACHUU3_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR2083693.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3334..3732
                     /gene="yciA"
                     /locus_tag="ACHUU3_003723"
     CDS             3334..3732
                     /gene="yciA"
                     /locus_tag="ACHUU3_003723"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR2083694.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHUU3_003724"
     CDS             complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHUU3_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR2083695.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4582..6102
                     /locus_tag="ACHUU3_003725"
     CDS             4582..6102
                     /locus_tag="ACHUU3_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR2083696.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6135..6905)
                     /locus_tag="ACHUU3_003726"
     CDS             complement(6135..6905)
                     /locus_tag="ACHUU3_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR2083697.1"
                     /translation="MFDTKQIRYSGKDGIYFHLRDNVESFLALTATEQMDYDSPMMVT
                     TQAMQSGQTITDNVQRAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQK
                     QIITIVCRDGIAITDSIITQFKASKEHTIANGLRIQMTFQGVEFRSIVGQTDVSAATG
                     KTATTNDGGATSKKNTGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSV
                     KNGESTFSDAANEQASKVLKPGNALRKYSVNPNKKGTY"
     gene            complement(7079..>7477)
                     /locus_tag="ACHUU3_003727"
     CDS             complement(7079..>7477)
                     /locus_tag="ACHUU3_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR2083698.1"
                     /translation="DVINAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNSL
                     AAVLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTV
                     LDGKVIDERTTSHINRMQEDTLISSAYPEE"
BASE COUNT         1980 a         1679 c         1728 g         2090 t
ORIGIN      
        1 tgtaaataaa atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat
       61 taacactgaa aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac
      121 tggcgacgag aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc
      181 aaattattgc tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca
      241 caacgattaa tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga
      301 aaagaatatc cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta
      361 ttgatgatac cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca
      421 gttactgaat tgtataccga cgttgtatgg gcgtcgttca acacactgca caataaaatt
      481 aatccgtata aggaaaactg atatggcaac tttgacaaaa aaagaacggg catggttgaa
      541 cgaattacag gaagttcttg atcgctgtcc tgtggtgtca tcctgccgcg attgcgttct
      601 tagtccgtgg ctattgcacc accatcacgc gaaagggaca gctaagcgcg gcgggatggt
      661 taagccagca acattaaccg ttgcatttaa aaaagcccgg gattctgtgg attacaactg
      721 gcgtgctaat ggcaccccac cctctttcca tgagcagaga tctttatcag agcgattgtt
      781 cagagagcag ggggttgata ccaaaatttt gctaggccat tcgaatcaaa aaatgaccga
      841 tatttacaac gatgtacgcg gtaaggagtg gaaaaaactg gtcatttgat aaccagtttt
      901 gcagaggttt tgcagagaaa attaaaagcg gtacccggca gagaacataa acacccacgg
      961 atccagtcgt accgagtctt ttacggtagt aacaccagat ttgtacttag ccgtggtatc
     1021 gatatccatg taccacactg acatgttaac caaccagtca cggttaatca gataatcaac
     1081 ccccacctgc ccggcagctc cccagtaatc tttcagactg agatcggaaa gccccgcctc
     1141 tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac ctgccccaac
     1201 ataaggacgg aatttgctgc cggcatcacc aaaataccac tgcgccatca gtgttggcgg
     1261 cagatgatga acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg
     1321 cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaggcc
     1381 cagttgcgtg ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc
     1441 ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc
     1501 aaaggcactg ccagagagaa gagttgttac tgccaaagcc gccactgtta actttttcat
     1561 atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa
     1621 tgatcctaca tagatcacat tacatatgat aattcaccct ttattgatct ggattaattt
     1681 agaagttgca ttgagaatcc tggttaacgt gatttaaatc aatttttaga tattttcgct
     1741 acgcaaatat taactcgttc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat
     1801 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct
     1861 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     1921 ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga
     1981 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     2041 cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg
     2101 ggttgttcga cctggttcag aatatgtcac cggaacaaca acaaattttg ctgcaggctt
     2161 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat
     2221 taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca
     2281 gtgcgccgat attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga
     2341 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac
     2401 cggtgatgct ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc
     2461 tgacttgggc gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     2521 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     2581 tggcgaacac cttgagcaac ctgatttcag ccatattgct catctatctg ttccgcctgt
     2641 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     2701 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     2761 ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt
     2821 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     2881 gacgctgttc ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct
     2941 gtttgcggga gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     3001 gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc
     3061 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     3121 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     3181 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     3241 gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg
     3301 cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag
     3361 ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc
     3421 tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc aaaagaaatt
     3481 gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg
     3541 gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc
     3601 attaatattg aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa
     3661 gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta
     3721 cctgttgagt aatagtcaaa agcctccggt cggaggcttt tgactttcta cttactgaat
     3781 ttcggtggtg ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc
     3841 cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg
     3901 cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac
     3961 tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg
     4021 gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc
     4081 cgtacttgat gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc
     4141 tacgggtttg acatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg
     4201 cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat
     4261 cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg
     4321 cggttcgaga tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag
     4381 ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca
     4441 gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgtattgta
     4501 gctattcggc attgtagcat tagccataac gtttataagt tcatctttaa atgataatca
     4561 attgcatggg agtttatcac cgtgagaacc gtaataacat acttgagatt ctcatcagcc
     4621 atacaaggcg ctgaaggtgc agattcaacc agacgacaaa acgacctgtt caagcagtgg
     4681 ctgaagaaga atggcgatgc tcaaatagta gcgtctttca gtgatgaagg gttgagtggt
     4741 tacaaaggta aacaccttac tggtcagttt ggtgacatgt tagcccgtat tgaggctggg
     4801 gagtttccag aaggtacaat tctgctagtt gagagtatcg accgtatagg ccgattagag
     4861 catcttgaaa cagaagcctt gatgaaccgc atcttaggca acgggataga gattcacaca
     4921 ttgcaagatg gccttatcta cacaaaggat gctttagccg atgaccttgg cataagcatc
     4981 atccagcgtg tcaaagccta catagcgcat cagaaatcta agcagaagtc tttccgtgtt
     5041 tctcagaaat gggggcaacg tgcaaaactt gcccttgctg gtgaacaacg tcttacaaag
     5101 atggttcctg gatggattga ccctgagact tttaaactca atgaacacgc tgagactgtg
     5161 agattgatat tcaaactgct gctagatggt gaaagtctgc ataacattgc acgtcacctt
     5221 caaagcaacg gtataaagtc gtttagtcgc cgtaaagatg ctaatgggtt ctctgttcac
     5281 tctgtacgca ccattctaag gtcagagaca acaataggca cgttaccagc atcacaacgt
     5341 aatgaccgcc ccgctatacc gaactactac gaaggtgttg tagatatacc aacgttcaat
     5401 aaagctcaag agattctcga caagaatcgt aaaggccgta cacctgcaag tgacaaccca
     5461 ctaacgatta acatcttcaa aggtctgttt aggtgtcagt gtggggctag tgtccatcct
     5521 accggaacaa agaataagta tgctggggtc tacaggtgca ataaccactt agacggtcgc
     5581 tgtgatgttc caccgttgaa gcgtaaaccg tttgaccgat ggatgattga taattttctg
     5641 gggatgattg acgtggggaa tgatggagaa tcagagagaa agattgcagc gttacagcat
     5701 gaggttgaaa ttgtcacagc cagaatcaag aaagctaccg ccctacttct tgagatggat
     5761 gatattgatg aactaaaaat tcagcttaag gaactgaacc agaagcgcac agaactacag
     5821 accacgattg ataacatgag gcgtaaggct tcactcactg ataaggaact accccagctt
     5881 aaagatattg accttatgac taaagcgggt cgtgtcgagt gtcagttgat tttgtccaag
     5941 catctaaaag ggcttacatt gggtaaggat tcggtaactg taacgctaca gaacgacact
     6001 gaaatcacca tacctacaaa cccgttaccg ctaaatgatg ggtcaccaat ctttgaaatt
     6061 gctgataaag agctactgga tattgacgct tatcaactgt aattttcagg ggctatgaag
     6121 ccccttttta tgtctcaata agtgcccttc ttgttagggt tcacagagta ctttcgcaga
     6181 gcattacccg gcttaagcac ctttgaagct tgctcattag cggcatctga gaaggtgctc
     6241 tcaccatttt taacgctaac gctcttagag cacgttacac gggcttttag cgcttcatct
     6301 gataactcac ttgccgaata gccaaaaagc tctttacagg tcagcatagg gcttccaagg
     6361 ctcgttgtag tgtttccggt gttcttttta ctggtagcac cgccatcatt cgtggtagcg
     6421 gtctttccag tagcagccga aacatcagtc tgacctacga ttgaacgaaa ctctacacct
     6481 tggaaggtca tctgaatacg aagaccgtta gcaatggtat gctctttact cgctttaaac
     6541 tgagtaatga tactatcagt gatagctatt ccatctctac aaacaatagt gatgatttgc
     6601 ttctggtctc gccattgttc aaccgtagaa ataaaggttt ctacaagttg cccctgtctg
     6661 gttaatagaa ctgaacccgt cgaatcaacc actacaacac cgctaataga gatggtctta
     6721 ggtgctctct gaacattatc agtgatagtt tgccccgatt gcatagcctg agttgtcacc
     6781 atcatcggtg agtcatagtc catctgttca gtagctgtta aggcaagaaa agattccaca
     6841 ttatctctaa ggtgaaaata gataccatct ttacctgagt atctaatttg ctttgtgtca
     6901 aacattgttg ttattcctat tctgttaagc gtgagctacg tgaaatctaa tttttgatgt
     6961 tggtatctgt tcagaagatt tcacgtagca ttcgactatt catagttgaa tcgtcatact
     7021 agataaagat taatagattg ccatgtgtgg caataaatat tagtagctct aacattgttt
     7081 attcctctgg ataagctgag ctaatcaagg tatcttcctg catacgatta atatgcgatg
     7141 tggttctttc atcaataacc tttccatcaa gtaccgtctg agttgtgata ttgattgtct
     7201 gaggctgaat atttactgac ggtttgataa cctttaaatc ttgtgtgaat gtttccactc
     7261 gacctttcag attatttaat gcttcaggtg aaaactgtgg aacatctttt gatttgccct
     7321 gaaccatagc ccataaaaca gcggctaaag agttcatgtc accagtcaat gcttggtctg
     7381 tgggttccac ctttgacctt acaactttat caattggatt atctgactta ctcatctgac
     7441 gttcatagaa cggtttgaca atggcattaa tcacatc
//
LOCUS       DAZPLI010000181         7415 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZPLI010000181 DAZPLI010000000
VERSION     DAZPLI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7415
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.182"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(75..1583)
                     /gene="putP"
                     /locus_tag="ACHUU3_003728"
     CDS             complement(75..1583)
                     /gene="putP"
                     /locus_tag="ACHUU3_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFR2083699.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            2005..5967
                     /gene="putA"
                     /locus_tag="ACHUU3_003729"
     CDS             2005..5967
                     /gene="putA"
                     /locus_tag="ACHUU3_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR2083700.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(6007..6645)
                     /gene="rutR"
                     /locus_tag="ACHUU3_003730"
     CDS             complement(6007..6645)
                     /gene="rutR"
                     /locus_tag="ACHUU3_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR2083701.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            6933..>7415
                     /locus_tag="ACHUU3_003731"
     CDS             6933..>7415
                     /locus_tag="ACHUU3_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR2083702.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         1771 a         2055 c         1943 g         1646 t
ORIGIN      
        1 tttatatggc gaggcacatt aagggagcgc aacccgctgc tgaagccaaa caaaaccgcc
       61 gcaggctaag tcccttagtt ttcctgcaac cgtgacggcg gagccgaatg atagtgcgca
      121 tcggcctcgg caaagcgttt ttgcatcgcc gctgacggcg ctttacccag caaactaaac
      181 actacaatcc cgatactgcc gaagatgaag cccggaataa tttcgtacag tcccagccag
      241 ccgaactgct tccagacgat aactgtcagc gcaccgatga tcatccccgc cagcgcaccg
      301 ttgcgcgtca tgcgtgacca catcaccgag aacagcacca ccggaccaaa cgccgcgcca
      361 aagcctgccc acgcgtagct cactaagccc agcacgcggt tttccgggtt tgccgccagc
      421 gcaatcgcca ccagcgccac caccagcacc atcacacgtc ctacccacac cagctctttc
      481 tggctggcct gtttacgcag aaacgctttg tataaatctt cggtaatcgc actggagcac
      541 accagcagct ggcaacttaa ggttgacatt accgccgcca gaatcgccga cagcagaatc
      601 ccggcaatcc acgggttaaa cagaatttgc gccagttcga taaacacgcg ttcagcgttc
      661 tggtttaccg cacctgctac cgccggatgc tcgttaaagt aagcgatacc aaagaagcca
      721 acagccaccg cccctgccag gcaaaggatc atccaggtca tactgatacg acgcgcatgg
      781 acaatgctgt ggtgagaatc tgccgccata aaacgcgcca ggatgtgcgg ctgcccgaag
      841 taacccagcc cccaacccat caatgagata atggcaacaa agttcagccc tttgagcata
      901 tccacgtttt cgatgctctt ttgtttgatc acttccagcg agtcaccaaa gccaccgaca
      961 ctgataatga cgataaccgg cgtcaggatc agggcaaaaa tcatcaggct ggcctgtaca
     1021 gtgtcagtcc agctcaccgc gaggaaaccg ccaataaagg tgtaaaggat cgtcgacgca
     1081 gctcccgcca acagagccgt ttcgtaactc atgccaaagg tactttcaaa cagacgcgcg
     1141 cctgccacaa tgcccgaagc gcaataaatg gtgaagaaca gcaaaataac cagcgcggag
     1201 ataatgcgca aaatgcggct tttatcttca aaacgcccgg tgaaataatc cggcaatgtt
     1261 aaggcgttat tattgtattc ggtatgcaca cgcaaccgcc cggccaccag cttccagttg
     1321 atccacgcgc ctaatgtcag gccaatggcg atccagcttt cggaaatccc ggaaagaaaa
     1381 accgcgccag gcaaccccat taacagccag ccgctcatat ccgacgcacc cgccgataat
     1441 gccgtcacga atggcccaag actacgaccg cccagaatat agtcgtcaaa gtttttcgtt
     1501 gatcgccagg cgataaaccc aatcaatatc atgccaaaga tatagacaca aaatgtcacc
     1561 aacatcggtg tgctaatagc catctaaagt ctccaaaaaa ttattatcgg caatgtcgaa
     1621 acttgccgtt atatctgcca ccggaacggg gtaacagagt ttatgtttta ccagggcgac
     1681 cgtatcctgc cggaagcgct ggttattcac aatcgattta acacaccatt tacattaaat
     1741 tttagtgctc agcgacacta tttttcatca ggttgcactc tctcacattt tttgcggttg
     1801 cacctttcaa aaaggttaac tgccgcagag aaaaagtctg agttattttt taatcactgt
     1861 catatcgatt tcttttatta acatttcatt catttttaag cttgctacgc aagtcacatt
     1921 taacatggtt gcacaaagtt gcaacatcat ggatatttca cgataacgtt aagttgcacc
     1981 tttcagaaca acaggagtaa tggcatggga accaccacca tgggggttaa gctggacgac
     2041 gcgacgcgtg agcgtattaa gtctgccgcg acacgtatcg atcgcacacc acactggtta
     2101 attaagcagg cgattttttc ttatctcgaa caactggaaa acagcgatac tctgccggag
     2161 ctacctgcgc tgctttctgg cgcggccaat gagagcgatg aagcaccgac tccggcagag
     2221 gaaccacacc agccattcct cgactttgcc gagcaaatat tgccccagtc ggtttcccgc
     2281 gccgcgatca ccgcggccta tcgccgcccg gaaaccgaag cggtttctat gctgctggaa
     2341 caagcccgcc tgccgcagcc agttgctgaa caggcgcaca aactggcgta tcaactggcc
     2401 gataaactgc gtaatcaaaa aaatgccagt ggtcgtgcag gaatggtcca ggggttattg
     2461 caggagtttt cgctgtcatc gcaggaaggc gtggcgctga tgtgtctggc agaagcgttg
     2521 ttgcgtattc ccgacaaagc cacccgcgac gcgttaattc gcgacaaaat cagcaacggt
     2581 aactggcagt cacacattgg tcgtagcccg tcactgtttg ttaatgccgc tacctggggg
     2641 ctgctgttta ctggcaaact ggtttccacc cataacgaag ccagcctctc ccgctcgctg
     2701 aaccgcatta tcggtaaaag cggtgaaccg ctgatccgca aaggtgtgga tatggcgatg
     2761 cgcctgatgg gcgagcagtt cgtcacgggt gaaaccatcg cggaagcgtt agccaatgcc
     2821 cgcaagctgg aagagaaagg tttccgttac tcttacgata tgctgggcga agctgcactg
     2881 actgccgcag acgcacaggc gtatatggtt tcctatcagc aggcgattca cgccattggt
     2941 aaagcgtcta acggtcgtgg catctatgaa ggtccgggca tttcaatcaa actgtcggcg
     3001 ctgcatccgc gctacagccg cgcccagtat gaccgggtaa tggaagagct ttacccgcgt
     3061 ctgaaatcac tcaccctgct ggcgcgtcag tatgatattg gtattaacat cgatgccgaa
     3121 gaggccgatc gtctggagat ctccctcgat ctgctggaaa atctctgttt cgagccggaa
     3181 ctggcaggct ggaacggcat cggttttgtt attcaggctt atcaaaaacg ctgcccgttg
     3241 gtgatcgatt acctggttga tctcgccacc cgcagccgtc gccgtctgat gattcgcctg
     3301 gtgaaaggcg cgtactggga tagtgaaatc aagcgtgcgc agatggacgg ccttgaaggc
     3361 tatccggttt atacccgcaa ggtgtatacc gacgtttctt atctcgcctg tgcgaaaaag
     3421 ctgctggcgg tgccgaatct aatctacccg cagttcgcga cgcacaacgc ccatacgctg
     3481 gcagcgattt atcaactggc aggtcagaac tactatccgg gtcagtacga gttccagtgc
     3541 ctgcatggta tgggcgagcc actgtacgag caggtcaccg ggaaagttgc cgacggtaaa
     3601 cttaaccgac cgtgtcgtat ttatgccccg gttggcacac atgaaacgct gctggcgtat
     3661 ctggtgcgtc gcctgctgga aaacggtgct aacacctcgt ttgttaaccg tattgccgac
     3721 acctctttgc cactggatga actggtcgcc gatccggtca ctgctgtaga aaaactggcg
     3781 caacaggaag ggcaaactgg attaccgcat ccgaaaattc ccctgccgcg cgatctttac
     3841 ggtcacgggc gcgacaactc ggcagggctg gatctcgcta acgaacaccg tctggcctcg
     3901 ctctcctctg ccctgctcaa tagtgcactg caaaaatggc aggccttgcc aatgctggaa
     3961 caaccggtag cggcaggtga gatgtcgccc gttattaacc ctgcggaacc gaaagatatt
     4021 gtgggctttg tgcgtgaagc cacgccacgt gaagtagaac aggcgctgga aagtgcggtt
     4081 aataacgcgc caatctggtt tgccacgcct ccggctgaac gcgcggcgat tttgcatcgc
     4141 gctgccgtgc taatggaaag ccagatgcag caactgattg gtattctggt gcgtgaagcc
     4201 ggaaaaacct tcagtaacgc cattgccgaa gtgcgcgaag cggtcgattt tctccactac
     4261 tacgccggac aggtgcggga tgatttcgct aacgaaaccc accgtccatt agggcctgtg
     4321 gtgtgtatca gtccgtggaa cttcccgctg gctattttca ccgggcagat cgctgccgca
     4381 ctggcggcag gtaacagcgt gctggcaaaa ccggcagaac aaacgccgct gattgccgcg
     4441 caagggatcg ccattttgct ggaagcgggt gtaccccctg gcgtggtgca attgctgcca
     4501 ggtcaggggg aaaccgtggg cgcgcaactg acgggtgatg atcgcgtgcg cggggtgatg
     4561 tttaccggtt caaccgaagt cgctacgtta ctgcagcgca atatcgccag ccgcctggac
     4621 gctcagggtc gccctattcc gctcatcgct gaaaccggcg gcatgaacgc gatgattgtc
     4681 gattcttcag cactgaccga acaagtcgtc gtggatgtgc tggcctcggc gttcgacagt
     4741 gcgggtcagc gttgttcggc actgcgcgtg ctgtgcctgc aagatgagat tgccgaccac
     4801 acgttgaaaa tgctgcgcgg tgcaatggcc gaatgccgga tgggtaatcc gggtcgcctg
     4861 accaccgata tcggtccggt gattgatagc gaagcgaaag ccaatattga gcgccatatt
     4921 cagaccatgc gtagcaaagg ccgtccggtg ttccaggcgg tgcgggaaaa cagcgaagat
     4981 gcccgtgaat ggcaaagcgg cacctttgtc gccccgacgc tgatcgaact ggatgacttt
     5041 gccgaattgc acaaagaggt ctttggtccg gtgctgcatg tggtgcgtta caaccgtaac
     5101 cagctaccag agctgatcga gcagattaac gcttccggct atggtctgac gcttggtgtc
     5161 catacgcgta ttgatgaaac catcgcccag gtcactggct cggcccatgt tggtaacctg
     5221 tatgttaacc gtaatatggt gggcgcagtg gttggtgtgc agccgttcgg cggcgaaggg
     5281 ttgtccggta ccgggccgaa agcaggcggt ccgctctatc tctaccgtct gctggcgaat
     5341 cgcccggaaa gtgcgctggc agtgacgctc gcgcgtcagg atgcagagta tccggtcgat
     5401 gcacagttga aagccgcatt gactcagccg ctaaatgcac tgcgggaatg ggcagcaaat
     5461 cgtccagaat tgcaggcgtt atgtacgcaa tatggcgagc tggcgcaggc aggaacacaa
     5521 cgattgctgc cggggccgac gggtgaacgc aacacctgga cgctgctgcc gcgtgagcgc
     5581 gtgttgtgta ttgccgatga tgagcaggat gcgctgactc agctcgccgc cgtgctggcg
     5641 gtgggcagcc aggtactgtg gccggatgac gcgctgcatc gtcagctagt aaaggcattg
     5701 ccatcggcag tcagcgaacg tattcaactg gcgaaagcgg aaaatataac cgctcaaccc
     5761 tttgatgcgg tgatcttcca cggtgattcg gatcagcttc gcgcattgtg tgaagccgtt
     5821 gctgcgcggg atggcgcaat tgtttcggtg cagggttttg cccgtggcga aagcaatatc
     5881 cttctggaac ggctgtatat cgaacgttcg ctgagtgtga ataccgctgc cgctggcggt
     5941 aacgccagtt taatgacgat cggttaaaca ggtagccgga ggatgttaca acctcctccg
     6001 gcatctttaa cgtggtcgaa tcccctcaat aataatccgc tgcacgtttt caaccgtttg
     6061 attgaaaaat atctcatcgc gcaacgtcgc gcctgtcacc gcctccacct gaggggcgaa
     6121 atcggcgtaa tgttgagtgg aagcccaaat catgaaaatc aaatgctgcg gatcaatcgg
     6181 cgcgagtttg ccgcttttga cccaaccggc aatcagcgca gatttctcat caattaatgc
     6241 cttcaaatcg cccgtcagtt catccattaa cagcggcgcg cctgccagca tctccataca
     6301 gaacaggcgc gaagcctgcg gataatcgcg ggagacttcc agcttcagac ggatgtactc
     6361 ttttatcgcc gccagcggag cgaaatcttc acgaaacgct tttaacggtg ccagccagat
     6421 atcgagaatc tgccgcagca cggcaatata cagcgcctct tttgacggaa agtaatacag
     6481 cagattggtt tttgaaacac ccgccaactc tgcgatctgc tccagccttg tgccgtgaaa
     6541 accgaattgt gaaaaagtgt ccagtgctgc gctaagaatc gcttttttct tcgcgcttac
     6601 tgcgcgcgaa cgtttacccg ttgttttcac tgcgccttgc gtcatgcgct ctcccctctt
     6661 gtagattggc aacaggatag caaaaccccc gcccggcctc gaccttctgc actctcatcg
     6721 cgcacagatg catgttttat gtgcaactgt tttgaccgtt tagtccactt tttatcagat
     6781 attttagata acgcaatatt aacatattaa taacattaac tttttaaaaa ctggcatccg
     6841 ctttgcaaac aagccaatac gcggctgaaa aggatatgca ggatgcagcg ccccgtctga
     6901 ctttcacttt acgagacgaa gagaggttga tgatgaaaat tggcgtattc gtacctattg
     6961 gcaacaacgg ctggctcatt tcgacccacg cgccgcagta catgccgacc tttgaactga
     7021 ataaagccat tgtgcagaaa gcggagcatt accatttcga tttcgccctg tcgatgatca
     7081 aactgcgtgg ctttggcggc aaaactgagt tctgggatca caaccttgag tcgttcacct
     7141 tgatggcggg tctggcagcc gtgacctcgc gcattcagat ttacgccact gctgccacct
     7201 taacgttacc tccggcaatc gtcgcccgta tggccgcaac catcgactcc atctctggcg
     7261 ggcgttttgg cgtcaacctc gtgactggct ggcaaaagcc cgtgtatgag cagatgggta
     7321 tctggcctgg cgatgactat ttctcccgtc gttacgacta tctcaccgaa tatgttcagg
     7381 tgctgcgcga cctgtggggc acggggaaaa gcgat
//
LOCUS       DAZPLI010000182         7392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZPLI010000182 DAZPLI010000000
VERSION     DAZPLI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.183"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(39..1298)
                     /gene="rho"
                     /locus_tag="ACHUU3_003732"
     CDS             complement(39..1298)
                     /gene="rho"
                     /locus_tag="ACHUU3_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR2083703.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(1383..1484)
                     /gene="rhoL"
                     /locus_tag="ACHUU3_003733"
     CDS             complement(1383..1484)
                     /gene="rhoL"
                     /locus_tag="ACHUU3_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR2083704.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(1625..1954)
                     /gene="trxA"
                     /locus_tag="ACHUU3_003734"
     CDS             complement(1625..1954)
                     /gene="trxA"
                     /locus_tag="ACHUU3_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR2083705.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            2085..3350
                     /gene="rhlB"
                     /locus_tag="ACHUU3_003735"
     CDS             2085..3350
                     /gene="rhlB"
                     /locus_tag="ACHUU3_003735"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR2083706.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            3484..4968
                     /gene="gppA"
                     /locus_tag="ACHUU3_003736"
     CDS             3484..4968
                     /gene="gppA"
                     /locus_tag="ACHUU3_003736"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR2083707.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(5015..7036)
                     /gene="rep"
                     /locus_tag="ACHUU3_003737"
     CDS             complement(5015..7036)
                     /gene="rep"
                     /locus_tag="ACHUU3_003737"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR2083708.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            7253..>7392
                     /locus_tag="ACHUU3_003738"
     CDS             7253..>7392
                     /locus_tag="ACHUU3_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083709.1"
                     /translation="MAINFSPKVGEILDCNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLV"
BASE COUNT         1753 a         1892 c         1902 g         1845 t
ORIGIN      
        1 gccacgtaaa cacgtggcgt ttttggcata agacaaattt atgagcgttt catcatttcg
       61 aagaaatcgt cattggtctt ggtcatggcc agtttattaa tgaggaattc cattgcatcg
      121 atttcgccca tcgggtgaat gattttgcgc aggatccaca ttttctgcag ttcttcctga
      181 gtagtgagca gttcttcttt acgggtaccg gaacggttgt agtcgatagc cgggaagacg
      241 cgtttttcag cgatcttacg agagaggtgc agttccatgt tgcctgtacc tttaaactct
      301 tcgtagataa cttcgtccat tttagaaccg gtatcgataa gcgccgtcgc gataatagtc
      361 aggctgccgc cctcttccac gttacgcgcc gcaccaaaga agcgtttcgg acgatgcagg
      421 gcgttggcat ccacaccacc ggtcaacact ttacctgacg ccggaacaac ggtgttgtaa
      481 gcgcgcgcca gacgagtgat ggagtcgagc agaatgataa cgtctttctt gtgctcaacc
      541 aggcgtttgg ccttctcgat caccatttcc gcaacctgaa cgtggcgaga tgcgggttcg
      601 tcaaaggtag aagcaacaac ttcacctttt accagacgct gcatctcggt tacttcttcc
      661 ggacgttcgt cgatcagcag aaccatcagc acacaatccg ggtggttgta agcaatgctc
      721 tgggcaatgt tctgcagcag catggtttta ccggctttcg gcggtgccac aatcagacca
      781 cgctgaccac gaccgatagg tgatgccaga tccagtacgc gagcagttaa atcttcagta
      841 gaaccgttac cacgttccat acgcagacga gaatttgcgt gcagcggggt taagttctca
      901 aagaggattt tgttgcgggc gttttcaggt ttgtcgaagt taacttcgtt aactttcagc
      961 agcgcaaaat agcgttcacc ttctttcggc gggcgaatct taccagagat ggtatcacca
     1021 gtgcggaggt tgaaacggcg gatttggcta ggggaaacgt agatgtcatc aggaccggcg
     1081 aggtaggagc tgtctgcgga acggaggaaa ccaaatccat cctgcaatat ctccagtacg
     1141 ccatcaccaa agatatcttc gccactcttt gcgtgctgct tcaggatggc aaaaataatg
     1201 tcctgcttac gcatgcgagc caggttttcc agccccatat tttcgccgag agtgatcagc
     1261 tcagaaaccg gcgtattctt taattcggta agattcatag tggtgtgagt tcttaaactt
     1321 ggggtaaatc tcgaacttaa tgttgtgaat ggtatggcag gatcatccat gcctgtttac
     1381 ggtcatcgac tcatgtcttt tcgctgcctg gtcacaggag agtacgcaga actgaaacga
     1441 caagacggat tgagtgacga gccagaaatc tgttcacttc gcatttaaga taaaacggga
     1501 agcgttggaa attacaagat tcaaacttaa taaggtatgt ttaatacgaa gtcaacacta
     1561 agttagcatg actcacgccg ggcgtccagt ttttagcgac ggggcacccg aacatgaaat
     1621 tcccttacgc caggttagcg tcgaggaact ctttcaactg acctttagac agtgcgccca
     1681 ctttggttgc cgccacttca ccgtttttga acagcagcag agtcgggata ccacggatgc
     1741 catatttcgg cgcagtgcca gggttttgat cgatgttcag ttttgcaacg gtcagtttgc
     1801 cctgatattc gtcagcgatt tcatccagaa tcggggcgat cattttgcac ggaccgcacc
     1861 actctgccca gaaatcgacg aggatcgccc cgtccgcttt gagtacatcc gtgtcaaaac
     1921 tgtcgtcagt caggtgaata attttatcgc tcatatataa ctccacagga ataagcctgg
     1981 cgtgttggtt tcgttgttgg tgtaacatta accaactaaa ggttgacttt atttcaccgg
     2041 atacgctttc gtaaagcaat agtaagctga tattctacca cactatgagc aaaacacatt
     2101 taacagaaca gaagttttcc gacttcgccc tgcatccgaa ggttgtagaa gcccttgaaa
     2161 aaaaagggtt tcataactgt acgcccattc aggcactggc ccttccgctg acgctggcgg
     2221 gtcgtgacgt agccgggcag gcgcaaaccg gtaccgggaa aacgatggcg tttcttacgt
     2281 caacgtttca ttatcttctc tctcatcctg cgattgccga tcgcaaggtg aatcaaccgc
     2341 gtgccttaat tatggcaccg acgcgtgaac ttgccgtgca gatccatgcc gacgcagaac
     2401 cgctggcgga agctactggc ctgaagctgg gtctggctta cggtggtgat ggctacgaca
     2461 aacagctgaa agtgctggaa agcggcgttg acattctgat tggcaccacg ggtcgtttaa
     2521 ttgactacgc caagcagaac cacattaacc tcggtgccat tcaggtagtg gttctggatg
     2581 aagccgatcg catgtacgat ctgggcttta ttaaagatat ccgctggctg ttccgccgta
     2641 tgccgcctgc aaaccagcgc ctcaacatgc tgttctccgc cacgctttcg taccgggtac
     2701 gtgaactggc gttcgagcag atgaacaatg ccgaatatat tgaagtggaa ccggaacaga
     2761 aaacgggcca ccgtattaaa gaagagcttt tctacccttc taacgaagaa aaaatgcgtt
     2821 tgctgcaaac gctgatcgaa gaagagtggc cagacagagc gattattttc gccaatacca
     2881 aacaccgttg tgaagagatc tggggccacc tggcggcaga tggtcatcgt gtcggtttgt
     2941 tgacaggcga tgtcgcgcag aaaaaacgtc tgcgtattct tgatgaattt acccgtggcg
     3001 atctggatat tctggttgcc accgacgttg ccgcgcgtgg tttgcatatt ccggcagtga
     3061 cgcacgtctt taactacgat ttacccgatg actgtgaaga ttacgttcac cgtattggtc
     3121 gtacaggtcg cgcaggcgca agcggtcact ctatcagcct ggcgtgtgaa gagtatgcat
     3181 tgaatttgcc tgctattgaa acctatattg gtcactcaat tccggtaagc aaatacaatc
     3241 cggacgcatt gatgaccgat ctgccaaaac cgctgcgcct cacgcgccca cgtacaggca
     3301 atggtccgcg tcgtactggc gctccgcgta atcgtcgtcg ttcaggttaa ataaaatgtc
     3361 atactcaaaa tcattcaaac tgtatcaaga tggcaagaga atgaatcccg atgagcatac
     3421 tgtagtaagt gattcaggtg aaagaatgca gccaacacag agacagattg aaggatgaag
     3481 agtatgggtt ccacctcgtc gctgtatgca gccattgatc tcggttcgaa tagttttcat
     3541 atgctggttg tgcgcgaggt ggctggaagc atccagacgc tgacgcgaat aaaacgcaaa
     3601 gtgcgtctgg ctgctggcct gaatagcgaa aatgccctgt ctaatgaagc aatggagcgc
     3661 ggttggcaat gtctgcgcct gtttgctgaa cgtctgcaag atatccctcc ctcgcaaatt
     3721 cgcgttgtcg ctacggcgac attacgtctt gccgtcaatg cgggtgattt tattgccaat
     3781 gcgcaggaaa tcctcggttg tccggtacag gtgatcagcg gtgaagagga agcacgtctg
     3841 atttatcagg gcgttgctca cacaactggc ggtgccgatc agcgcctggt ggtggatata
     3901 ggcggtgcca gtactgaact ggtaaccggc acgggtgcac aaaccacctc gttgttcagc
     3961 ctgtcgatgg gctgcgtcac ctggctggaa cgctattttg ccgatcgtaa tctggggcag
     4021 gaaaattttg atgctgcaga aaaagcggca cgcgaagtgt tacgtccggt tgccgatgaa
     4081 ttacggtatc acggctggaa agtgtgcgtt ggtgcttccg gcaccgtgca ggcgttacag
     4141 gaaatcatga tggcacaggg gatggatgaa cgcattaccc tggaaaagtt gcagcaattg
     4201 aaacagcgag ccattcattg cggtcggctg gaggaactgg agattgacgg gctgacgctg
     4261 gaacgtgcgt tagtgttccc gagtggtctg gcgatcctga tcgccatttt taccgaactg
     4321 aatattcagt gtatgaccct ggcgggcggt gcgctgcgtg aaggcctggt ctacggcatg
     4381 ttgcatctgg ccgtcgagca ggatattcgc agccgtacgc tgcgtaatat tcagcgccgc
     4441 tttatgatcg atattgatca ggcgcagcgc gtagccaaag ttgcggctaa cttcttcgat
     4501 caggtggaaa aggaatggca tcttgaagca ataagccgcg atttgctcat cagcgcctgt
     4561 cagcttcatg aaatcggcct gagcgttgac tttaaacaag cgccgcaaca cgctgcttat
     4621 ctggtgcgta atctggatct tcccggtttt acccccgcac agaaaaaatt gctggcgacg
     4681 ctactgctca accagactaa tccggtcgat ctctcatcgc tgcatcagca aaatgccgta
     4741 ccgccgcgcg tcgcagaaca actctgccgt ttactccgcc tggccatcat ttttgccagc
     4801 cgtcgccgtg acgatctcgt gccagagatg acattacagg ctaaccatga actgttgacc
     4861 ttgacgcttc cgcaaggttg gctaacccaa catccgctgg gtaaagagat tattgctcag
     4921 gaaagccagt ggcagagcta tgtccactgg ccgctggaag tgcattaatg agtaagtgcc
     4981 ggatgcgatg ctgacgcatc ttttccggcc ttgattattt ccctcgtttt gccgccatca
     5041 tcgctttcag attcgccaga tggctttgcc ctttctgcat ccgttcttcg gcgctgacca
     5101 ctttgcgctc ctgttcccaa atcagatcat cctgcggcag ctccagcaaa aagcggctcg
     5161 gctccgggcg caccagctcg ccgtactgac ggcgttcttt acacagcgta aaggtcaatt
     5221 ccttctgggc gcgggtaatg ccgacatagg ccagccgccg ctcctcatcg atattatctt
     5281 catcgatgct gctctggtgc ggcaaaaacc cttcttccat accgaccatg tagacataag
     5341 gaaactccag cccttttgac gcgtgcagag tcatcagttg cacctgatcc agctcttctt
     5401 cactctcacc acgctccatc atgtcgcgca aagtaaagcg cgtcaccacc tgggtgagcg
     5461 tcatcggctc atccagttcg ctgccttcca gcatctccgt catccagcta aacagttggt
     5521 tgacgttctt catgcgcatt tcggcggctt tcgggctggg cgatgtttcg tacagccagg
     5581 attcataatc catgccgtgg atcagatcgc gcaccgcggc aatcggctcc cgctccgcca
     5641 gacgctggat ttctgccaac cagtgagtaa agcgggtcaa tgcttcataa ccacgtccgc
     5701 taagcgtctg actcaggccc atatcaaagc tggcggtaaa catgctttta ttgcgcgtca
     5761 tcgcccactc acccagcttt ttcagcgtcg ccgggccaat ctctctcttc ggcgtgttaa
     5821 cgatacgcag aaatgcgctg tcatcgtccg ggttagtcag cacgcgcaga taagccagca
     5881 agtccttgat ttcaggacga gagaaaaacg acgtaccacc agaaattttg tacgggatgc
     5941 ggttttgcat caggaatttt tcaaacactc gcgactgatg gttaccgcga taaagaatag
     6001 cgtaatcttt gtactgcgtt ttattgacga agtgatgcgc aatcagttca ccagtgacgc
     6061 gctcggcttc atgttcttcg ttattcgcgc ttaaaacttt aagctctgtg ccgtaaccca
     6121 gttcggagaa cagacgcttc tcaaagacgt gcgggttatt ggcgatcagg atgttcgccg
     6181 ctttcaaaat acgtccggaa gagcggtagt tctgctcaag cttaatcacc ttcagcgccg
     6241 gaaaatcctg actcagcagc accaggtttt gtggacgtgc gccgcgccag gagtagatcg
     6301 actggtcgtc atcgcccacc acggtaaagc gcgcgcggct gcccaccagc agtttcacca
     6361 gttcatactg gctggtgttg gtgtcctgat actcatccac cagcagatag cgaattttgt
     6421 tctgccagcg ctcgcggact tcttcattgc gttgcagcag caacgtcggc aataaaatca
     6481 gatcatcgaa gtcgagaaca ttgcacgctt tcaggtgtgc atcatacagc ccataacaat
     6541 gggcaaaaat acggtcccgc tcgccaatcg cactggctgc cgcctgggac ggtgttttga
     6601 gatcgttctt ccagttagag atggtcgaaa tcaactgttg caggagaact ttgtcatctt
     6661 caatcagccc ttcggtcaac tctttgagca acgccagctg atcggtatcg tcaaacagcg
     6721 aaaagttcgc cttcatcccc agcgccgcat actcacgctt gatgatatcc agccccagcg
     6781 tgtggaaggt tgagatcatc agcccacgcg cctctttgcg acccagcgtc tgtccgacac
     6841 gctctttcat ctcgcgcgcc gccttattgg taaaggtcac cgccgcgata tgtcgcgcct
     6901 gataaccgca accgcggatc agatgggcga ttttattggt gatcacacga gttttaccgg
     6961 aacccgcgcc cgccagcacc aggcaggggc cggtaacgaa ttcgacagct tgttgttggc
     7021 cggggtttag acgcataggt gtattgctca atcttcgaac ggggggagga ttgtagcaga
     7081 atcgcatttg aatgtgaccg cgtgtaagcc gctaaccgat tggctgcgga tgtttaaatc
     7141 agagagggaa gaccgaaacc ggagttcatc gtgaatccat tagacttaga aaatatcggg
     7201 tcgtaacgtt gtgttattac tttttcagag tttactaagg agaggtgtac tcgtggcgat
     7261 taatttcagt cctaaggttg gtgaaatact ggattgcaac tttggtaact atcctgttag
     7321 ccagaatgga cagttcagca ccacgtatta cgatggccgc attcctcctg aaatgataaa
     7381 aaacaggtta gt
//
LOCUS       DAZPLI010000183         7366 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZPLI010000183 DAZPLI010000000
VERSION     DAZPLI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7366
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.184"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..959)
                     /locus_tag="ACHUU3_003739"
     CDS             complement(<1..959)
                     /locus_tag="ACHUU3_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR2083710.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEE"
     gene            complement(1560..1808)
                     /locus_tag="ACHUU3_003740"
     CDS             complement(1560..1808)
                     /locus_tag="ACHUU3_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR2083711.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2071..2166)
                     /locus_tag="ACHUU3_003741"
                     /pseudo
     CDS             complement(2071..2166)
                     /locus_tag="ACHUU3_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2163..2729)
                     /locus_tag="ACHUU3_003742"
     CDS             complement(2163..2729)
                     /locus_tag="ACHUU3_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR2083712.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            3039..4811
                     /gene="aspS"
                     /locus_tag="ACHUU3_003743"
     CDS             3039..4811
                     /gene="aspS"
                     /locus_tag="ACHUU3_003743"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR2083713.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            4929..5381
                     /gene="nudB"
                     /locus_tag="ACHUU3_003744"
     CDS             4929..5381
                     /gene="nudB"
                     /locus_tag="ACHUU3_003744"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR2083714.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            5410..6150
                     /locus_tag="ACHUU3_003745"
     CDS             5410..6150
                     /locus_tag="ACHUU3_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR2083715.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            6185..6706
                     /gene="ruvC"
                     /locus_tag="ACHUU3_003746"
     CDS             6185..6706
                     /gene="ruvC"
                     /locus_tag="ACHUU3_003746"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR2083716.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(6708..7310)
                     /locus_tag="ACHUU3_003747"
     CDS             complement(6708..7310)
                     /locus_tag="ACHUU3_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR2083717.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1875 a         1646 c         1931 g         1914 t
ORIGIN      
        1 tgctcttcat gttcaaaagc atgccatatc tgtgatacat cagattgttt gttttccggg
       61 aaccatgctg tcacggcatc agccaggggg cgatggagtg tattctgttg tccgtcactc
      121 atggagaagt aaatctgcgg gccgtggtag tccggcgaag aggttaatct ttgcagggat
      181 tgcagagcat gggatgacaa tgggttatca ctaatatcta ttgaacattc attcctcaga
      241 ttaagaatgc tttccgggat atgacttatc tgatttatgt caaaataacc gttccttaaa
      301 ttttccggca ggtatggaat atcggttaac atattgtcga ttgcccatat ggagtgaatg
      361 ttgtgaggta gaggggggag ttcactaaga agattcccta ttgcgctaat ctcttgtaaa
      421 gaaaatggta aaggtggtaa aactgccagc ccattacatg atacatctaa tgtttccagt
      481 agttctggta aaacaggaag agaacataat tgattatttg agacatgaag ctccttcaaa
      541 gatatgggga gtgtgggtag tgtgattagt tgattgtggg acgcattcaa taatttaagt
      601 ccttgaggca aagcaggcag ttcaataagt ctgttatagc tgacattaag ctgtgtaagg
      661 gacgcaggca atggggagat taagcttaaa ttatttttac ttatattaat tgatttaatt
      721 cccgggggga tttcaggtaa tgttgtcagg cctaattcag acaagtccag gttcgtctct
      781 tggttttgta gacatgatac tagtcgctga aaagcgatgt ctcgttgttc ttcttgtatg
      841 cggttatttt tccattcagt ccaatgggtt agataatttt catatgcacg gctagtgtcg
      901 attgaataag tggagaacga atttgaaatt aatctgtgat tgttatttgt cgggagcata
      961 aatatcaggg cgtattcttt atgttgagag gtgttggatt cttttttatc tgctttgcaa
     1021 tgttattggt tcccttcagc agtagacagg aacttctgga ggaggcggat atatagtttg
     1081 tgtaagcata ttaactccat atgttatata ttgaagaact tctgctttac actatcctaa
     1141 actggatcgg tttaatgtaa aaaaacgata ctttttgggg gagggggcaa tttttcctta
     1201 aggtatgaaa aaaggagctg aagctatata atagctatta atgccactgg tatcagtgaa
     1261 tcatgtatat aaaaaactgt gttaaggagg ctgtccatta gcggtttttt attcttctgg
     1321 aaccgcggca tctttgcgta tctttttgtc ttctcaccgt ctggtcggtg ttttgctgag
     1381 actgctaatt tcctgtcttt gctagtgctg tactggtact gcccaatcat gattggcggg
     1441 ggcgggcgga gttgagaccg cagacacgtc gtatgcaaga acgtgctgcg gctggctggt
     1501 gaacttccga tagtgcgagt attgaatgat ttccagccgt tactgatttt acgtgctaat
     1561 tagtgaacaa accactcgtc agcagactcc caggtatctt tcagagtttc ctgaacaaaa
     1621 gttttagctg aatctttatc ggcggtgcgc gtaacagaaa ggccatcgtt gctggtggct
     1681 tttacgatca cctctacatc gtcataacgt ttactgatgc gtcgggttaa ttcttccttt
     1741 aacgcatcca cagcaccggt tggcatttta gtcattttct ctttggctat gcagatttca
     1801 atacgcataa aaatccctct acactgtgtt tttatacagt attattttta actgtatgga
     1861 taaacagtgt caagagtgag ggaaagtgat tttttggggc atgcatgggg caaaaatatt
     1921 tgttgtgggg cattttgggg catgattggg acactttgtt acatatgaac tttgccgatt
     1981 ttcatatggc atggcaatcc aacgctttga ttattctaaa gaaaacacat gctcttgggc
     2041 gttctttagt gatttttaaa aatcccgtat cacgcagtta aagtggcggg catactcttc
     2101 aaggctggtg atccccaacc gcacccattt aggatgcgac cattgcggta gaccgatgta
     2161 aatcataacg cgtgaaggat ctcttccacg ctacgcacac gggcgatgcg cgggtagata
     2221 tgattaatgc tgttattgtg ttgctcggcg ctggcggcac tacaggcgtc ttcggcaatc
     2281 accagattaa agccaagttc ccaggcattg cgggcggtgg attcaacacc gatattggtc
     2341 gagatcccac ataacactat tgtatcgata cctcggcggc gcagttgcaa ctccagatcc
     2401 gtaccgtaaa acgcacccca ctgacgtttg atgatttcga tatcactgtc agttgcacct
     2461 aatgcagctg gatgttgcca ccagttttcg ggcaacactt tagcggggga gggggcatca
     2521 accggctgtt ttaatgcttc ggcgtaatcg gcagaccagc caactcgcac cagaaacacg
     2581 ggctgaccgc tggcgcgaaa tttcgccgcc agcttcccgg cgcgattaac tacctcatcg
     2641 gcagtatgtg gaccaccggc aaaaggtaag atgccttctt gtaaatcgat caccaccaac
     2701 gcagtggttt tagcattaag ttcaagcatg ctgactcctg aaaaaacaaa atcgtgcctc
     2761 acaccttaat gcgattagcc gatggcggaa cgacaaattt tgttaatttt tatgagttcg
     2821 ggcaacacct ttatagcaaa ctggcgtaga gtctgatttc aggcccgata taaagtggta
     2881 acgaggcgtt acagcgcggg ccaggctctt acggtgcggc agaatttcca gtataatagc
     2941 cgcctttttt cattcagttg tgacatacag ctaacgctgc gacttggtca cctgcgcagc
     3001 agggggcatc cgccggcagc taagttaagg gatatctcat gcgtacagaa tattgtggac
     3061 agctccgttt gtcccacgtg gggcagcagg tgactctgtg tggttgggtc aaccgtcgtc
     3121 gtgatcttgg tagcctgatc ttcatcgata tgcgcgaccg cgaaggtatc gtgcaggtat
     3181 ttttcgatcc ggatcgtgcg gacgcgttaa agctggcctc tgaactgcgt aatgagttct
     3241 gcattcaggt tacgggcacc gtacgtgcgc gtgacgaaaa aaatattaac cgcgatatgg
     3301 cgaccggcga aattgaagtg ctggcgtcct cgctgactat catcaaccgc gcagatgttc
     3361 tgccgcttga ctctaaccac gtcaacaccg aagaagcgcg tctgaaatac cgctacctcg
     3421 acctgcgtcg cccggaaatg gcgcagcgcc tgaaaacccg cgctaaaatc accagcctgg
     3481 tgcgccgttt tatggatgac cacggcttcc tcgacatcga aactccgatg ctgaccaaag
     3541 ccacgccgga aggcgcgcgt gactacttgg tgccttctcg tgtgcacaaa ggtaaattct
     3601 acgcactgcc gcaatcccca cagttgttca aacagctgct gatgatgtcc ggttttgacc
     3661 gttactatca gatcgttaaa tgcttccgtg acgaagacct gcgcgctgat cgtcagcctg
     3721 aatttactca gatcgatgtg gaaacttctt tcatgaccgc gccgcaagtg cgtgaagtga
     3781 tggaagcgct ggtgcgtcat ttgtggctgg aagtgaaggg cgtggatctg ggcgatttcc
     3841 cggtaatgac ctttgcggaa gcagaacgcc gttatggttc tgataaaccg gatctgcgta
     3901 acccgatgga actgaccgac gttgccgatc tgctgaaatc tgttgagttt gcagtatttg
     3961 cggggccggc gaacgatccg aaaggccgtg ttgccgctct acgtgttccg ggtggcgcat
     4021 cgctgacccg taagcagatc gacgaatacg gtaacttcgt taaaatctac ggcgcgaaag
     4081 gtctggctta catcaaagtt aacgaacgcg cgaaaggtct ggaaggtatc aacagcccgg
     4141 tagcgaagtt ccttaatgca gaaatcatcg aagccatcct ggatcgtact gccgcgcaag
     4201 atggcgatat gattttcttc ggtgccgaca acaagaaaat tgttgccgac gcgatgggcg
     4261 cactgcgcct gaaagtgggt aaagaccttg gtctgaccga cgaaagcaaa tgggcaccgc
     4321 tgtgggttat cgacttcccg atgtttgaag acgacggtga aggcggcctg acggcaatgc
     4381 accatccgtt cacctcaccg aaagacatga ctgctgcaga actgaaagct gcaccggaaa
     4441 atgcggtggc gaacgcttac gatatggtca tcaatggtta cgaagtgggc ggtggttcag
     4501 tacgtatcca taatggtgat atgcagcaga cggtgtttgg tattctgggt atcaacgaag
     4561 aggaacagcg cgagaaattc ggcttcctgc tcgacgccct gaaatacggt actccgccgc
     4621 acgcaggtct ggcattcggt cttgaccgtc tgaccatgct gctgactggc accgacaata
     4681 tccgtgacgt tatcgccttc ccgaaaacca cggcggcagc gtgtctgatg actgaagcac
     4741 cgagctttgc taacccgact gtactggctg agctgagcat tcaggttgtg aagaaggctg
     4801 agaataactg atatgactca aatacacgaa atcattcgcg ttgcatcaag gcggcaactg
     4861 agtgaactcc catgagcata gataactatg tgaatgggat gagcgaaggc agtcaacgaa
     4921 gaggcagcgt gaaggataaa gtgtataagc gtcccgtttc gatcttagtg gtcatctatg
     4981 cacaagatac gaaacgggtg ctgatgttgc agcggcgtga cgatcccgat ttctggcagt
     5041 cggtaaccgg cagcgtggaa gagggtgaaa ccgcgccgca agctgccatg cgcgaagtaa
     5101 aggaagaggt caccattgat gttgtcgctg aacaactgac cttaattgac tgtcagcgca
     5161 cggtagagtt tgaaattttt tcacatttac gtcatcgcta tgcgccgggc gtgacgcgta
     5221 atacggaatc atggttctgt cttgcgctcc cgcacgaacg ccaggtagtc ttcactgaac
     5281 atctggctta caagtggctt gatgcgcctg ctgccgcggc gctcactaag tcctggagca
     5341 accggcaggc gattgaacag tttgtaatta acgctgcctg aacaggcagg gctatctgga
     5401 gatattttta tggcaggtca tagtaaatgg gccaacacca gacatcgtaa agctgcgcag
     5461 gatgctaagc gcggtaaaat cttcactaaa atcattcgtg agctggtaac cgcggctaag
     5521 ctgggcggtg gcgatccgga cgctaacccg cgtctgcgtg cggcggtgga taaagcactg
     5581 tctaacaaca tgacccgtga cacactgaac cgcgcaattg cacgtggtgt gggcggtgat
     5641 gatgatgcaa acatggaaac catcatctac gaaggttacg gtcctggcgg cacggcaatc
     5701 atgattgaat gtctgtctga caaccgcaac cgtaccgttg ctgaagtgcg tcatgcattt
     5761 agcaaatgtg gcggtaacct cggtactgat ggttccgttg cctatctgtt cagcaaaaaa
     5821 ggcgtgatct ccttcgagaa aggcgatgaa gacaccatta tggaagcagc actggaagca
     5881 ggtgctgaag acgttgtgac ctatgatgac ggcgcgattg acgtctacac tgcatgggaa
     5941 gaaatgggta aagtgcgcga cgctctggaa gcggcaggtc tgaaagcaga cagcgcggaa
     6001 gtttccatga tcccgtccac caaagctgat atggatgcag aaaccgcacc gaaactgatg
     6061 cgtctgatcg atatgctgga agattgcgac gacgtgcagg aagtctacca taacggtgaa
     6121 atctctgatg aggttgcagc aactctctga tggggcctgc taaacagcaa aacggagacg
     6181 cgtgatggct attattctcg gcattgaccc gggttcgcgc gtgatcggct atggtgtcat
     6241 ccgccaggta ggcaggcaac tgtcctacct gggtagcgga tgcatacgca ccaaagtgga
     6301 tgatttaccg tctcgtctga agctcatcta tgcgggcgtg acggaaatca tcacccagtt
     6361 ccagccagat tatttcgcca ttgaacaagt ctttatggcc aaaaatgccg actcggctct
     6421 gaaactgggg caggcgcgcg gcgtggcgat tgtggcggcg gtgaatcagg aattgccggt
     6481 atttgaatac gcggcacgtc aggtaaagca aacggtggta ggtattggta gtgccgaaaa
     6541 aagccaggtg cagcatatgg tccgcacctt gctgaaactg cccgctaatc cacaggcgga
     6601 tgccgccgat gcgttagcta tcgcgattac ccattgtcac gtcagccaga atgcaatgca
     6661 gatgagcgaa tcgcggctga acctggcgag agggcgactg cgttaagtta taccgcctcg
     6721 gtcagttcag gctgaggcgt ttcaactccc tccgcgtgaa tcaacaccag acccggatga
     6781 tgccacaaac tggctggcgt gacggtttta cgctcccacg gaatagaacc taagaaccag
     6841 aatttccaga aagtgagttt tgcattcgga ttgctattta acagttctcc aaacgtttct
     6901 atttcaccca ccggaatgca tagcgcttct ttataaatat caaaaacaaa ttttgaacag
     6961 aactggcgcg aagattcgta tttaaaaccg gtgtggtaaa gtttgcgtag ccgggaggga
     7021 acctgttcaa caattcgttg tttttgttgt tctgtcagcc cagcgtctaa tcgctttata
     7081 gcatagcgtt gattagacga acgtttaata aaacgagata gcgtggtgat ggttgaaagg
     7141 ggaacccggc tttctgcaac caggaagtct tcaccgttat gaccgataat gatcccgacg
     7201 tgattactcc agcaatttga tgcagctgat atttgaccaa ataaggcagc acctatacat
     7261 gtaaagacga tatctccaat ttcatattca gcagggtaat taatattcac gttgtcattc
     7321 cattgaaata gatacgaata tacgtaatag cataaaaaat ttattt
//
LOCUS       DAZPLI010000184         7280 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZPLI010000184 DAZPLI010000000
VERSION     DAZPLI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7280
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.185"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(1..4518)
                     /locus_tag="ACHUU3_003748"
                     /pseudo
     CDS             complement(1..4518)
                     /locus_tag="ACHUU3_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(4538..4999)
                     /locus_tag="ACHUU3_003749"
     CDS             complement(4538..4999)
                     /locus_tag="ACHUU3_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFR2083718.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5027..6929)
                     /locus_tag="ACHUU3_003750"
                     /pseudo
     CDS             complement(5027..6929)
                     /locus_tag="ACHUU3_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
BASE COUNT         1381 a         2214 c         1962 g         1723 t
ORIGIN      
        1 ttatttgact acgtgataaa cgaaccccat ccaggaaacc cgtatgccca ctaacacgac
       61 gaccatcgcc aatatagtcc atcaaactgc tgattttaga acttgcagcc aatcccagcg
      121 gatcaaccca cccacaagga ttcggcacat accggtaagt ctgctccccg ccctgcaacc
      181 ccagcggatc actcaccagg tacattccgg tttccggctc gtaataccgg aaccgattat
      241 agcacagccc ggattccgca tcctgccact gtcctgcata cagcaggccg gggtcggctt
      301 ccgggtccag ttccccgcgc gggtccgggt agccggtgtc tgcgggcagg ctgagtgcca
      361 gcccccacag gctggtctgc cccggtcgcc agaccgtctt tccttcactg ttaaagagca
      421 tcagcgggcg gcccgtcagg tcgctcaccg catgattcgt ccgcgtcacc cactgcgggg
      481 ccaccgacgg atgcgcctgc cgtacccggc tgaactgctg gcttatcagc tcaaagccgt
      541 taaacaccag gtgccttacg ctgtacagtt tatcatcgcg gtattcccgg atttccgcaa
      601 tactgtcgcc gtcccacaga tacgtgaagc ggattttctt ccggtcgcag cgtttctctg
      661 ttcgtctgcc gctggcgtcg tggcggtatt cccacagctc accctgtgcg ctgcaatacc
      721 cggtcagctg gtcccggctg tcccaccgga accgctctgt ttccggtcgg tagccgtcac
      781 ggtgtctggt gcggctgacc atccggcctg cggcgtcata gtcatactgc gtgtttccgg
      841 cctgtttcag ccggtggcct ccggcatact ggtcagtctc ttcacttatc cggtgcctgc
      901 ccgcagactg cgccttcagg tagccgcagc tgtcatagcc ataggactca tcgccttcgc
      961 catagcccag cccggtgcct gaggctgtca ccgccgtgac ctggccgttc cggtccagcc
     1021 gatactcccg ccgtgtttcc cgtccgtaat cttcccggct ggtgacgcct gacacgttac
     1081 ccgcgctgtc gtaacggtac tcccgtacca gtgagggaac cagcccgtcc catgtggctt
     1141 ctgccggaaa aaatccggcg cgctgactga caggctgacc ggctgcattc cagccctgtt
     1201 cacaggcaaa accccggtta ctgtgccgga gcacttcacg tcccgcctca tcacggctga
     1261 acgtcagcgt ctcatccccc ggcagaatga cctctgtcag ttctccggcg gcgttgtagc
     1321 ggtacgtggt tttcagcagg gattcaccgg ggtgtctgtc gcgttcatct tccggtggca
     1381 ggatacagcg gatgacggtg tgttcatcgg ggtagttgta ttcaatggcc cgtcggccct
     1441 gagtggtgac attcacccgc ccgcgttcgt catagccggt gcgctgtgat gtgccgtccg
     1501 ggctgaagat acccgtcagc ctgccggatt tgtcataggc atactgcgtt ttgccttccg
     1561 gcgagatgat ttctgccagg cggcctgcgg cgtcccggct gtagaggatg gtttcctcca
     1621 ggccgttaac cctggcggta cagaggccat cggcgtcata gcggtaacgc cagaccacgc
     1681 cgccccagtc acgctcttcc gtcacccggc cgcagttatc cagaacataa agatatgact
     1741 caccctgcgg gttgatgact tctgtcagtt gcagcgtgtc tttatcgtag cggtactgcc
     1801 agcggtggcc ttcaccgtcc gttcgcgcca ccggaaaatc gaacggaccg tattccatcg
     1861 tccagacaac accgttgccg tccgtccagg ccagcaggtt gccgtggcgg tcatagcgaa
     1921 atccttccgc gccggttcca gcacgttcaa tgccgctcag tttatggtgc tgctgccact
     1981 gtaaccgcac tgactgaccg ccggggccgc gcatcttcac tggcaggtcg tgaccgctgt
     2041 attcggtgtg gtgttcacgg cccatcacgt cggtgagtgt ttccggccgg ttgtggtggt
     2101 cgtactctat gcccagacgc cgttcatcct ggcggaatat tcccgacagc agcccctgag
     2161 cggtgtatgc cagccgggtc tcttcgccct gtggcgacac cagacttacc ggcaggccgc
     2221 gttcatcaca ctgctgtgtc cacgtctgcc cggcagggtc ggtgatgttg ctgagcaggc
     2281 cgcgctcatc atagcggtac tgccactccg cgccgtctgc cgccgtgtaa cggcttaact
     2341 gaccgtgtgc ggcgttgtat tcaaatttct ccacccgccc ggcaggggag tggcgggcca
     2401 gcaggtgatg gaattcatcc cattcataca tggtggtgct gccgtccggc gcttcttccc
     2461 gcagaatatt gccgtcagga tcatagtaat aacggtgaat gcccttgccg tccgtcacgg
     2521 tggtgcagcc gtcaccatag tccagcgtgg cgttgtaata gccctccgcc ccggtgacat
     2581 tcacgcaccg gccctgttca tcgtaggtga aacggctcca cgtctggtcg ttatcggacc
     2641 agcggatgat ccggtccgca gcgtcgtact cataaaacag gtggtagtcc agccgcgcat
     2701 ccgcttctgt cagccgcccc cgcgcatcct gttcgtaggt cgccagcgtc tgtatgtcgt
     2761 tatccgtgcg gtgaatggct ttcaggtagc cgctggcgtg gtacgtcagt ttaagccgga
     2821 tcccgtcggt gtgactgaca cccgtgatcc ggtactggct gtcgcgctca aacgtgatgc
     2881 ggttgctggc ggtgtcgctc atcccgccca gcatgtacat ccggtcattg gggctgtgtg
     2941 ccaggcggtc atcgctggta tcgtcagtca ccgggtggcg cagacgcatt cccggaaagg
     3001 cgtcataaaa gaagcggctg ctcagtgcat ccctgtcgaa cagctccaga taatcatcgc
     3061 ggcggcgcag aatgtactgg gcgtgatacg ggttaaccgc cgtatcactg tcaccgtcga
     3121 aggcaaatct cagcgtggct ccctggctga tgatatccac ccggtttccc tgttcacgca
     3181 cccaggcgta ttcgctccag gagtcgctcc agcccacgcc ggtcaggcca tgcagcggca
     3241 gtacagaact gtggaaacgg gtgaaggaga gggggagggt ctggcccagg gtgaagtcgg
     3301 tacgttcgtc gcagtatgcg ccggttaccg gatcgacagg atctgttacc catctgatga
     3361 ctttattggc aatacttttt aacgacttac ctgttttccc cagcaacgcg ccgagctttg
     3421 cgcccagcag cttcccgctt ttccccagca gtttcccagt ttgccgagtc tcgccagatt
     3481 cttcagtttt cccagtagtc ccgctggcgg cagaagatag ctggctatcg ttattccccc
     3541 caggatcatt ctctgccacg gcgggaactc cggctggata tccagatatg tgacctgctc
     3601 accgccaata aagacgtccg gcgaacctcc cacgatcgcc gcactgcact ccagtttgtc
     3661 gcccttgcgc gcggcaggct gattgttgat aaagacgttg ctgctgccct gggctatcct
     3721 cggtggtttg ttctccttgt cgcagagcgc cgtgtcctca ccggcttccg ggaaatccac
     3781 atcttttttg atttcgccac gcccgcccac catggtggca aactccatta ataccggacc
     3841 ggcgggaaac agaagcgatg cgccggcgct gacgcgatcc ataaaaccgg cattgctgtc
     3901 cgtcagggtg ataacagcgt tcccccaggc cttcgccagt ggtactgctt tttccgctgc
     3961 cgattcaagc aggtcagtgg caatatcacc ccatgagggt tccccctgtg ggacttcagg
     4021 tattgctcca ggtgggggag gagggggaag ggtgaccgct gcacgtgcgg ccagctcgcc
     4081 gttaattttt acgttttcag aaccggtatt gatggcgccg tccgtgtcac aaaagctgtt
     4141 tatccatgat tcaagctggt tctgtttctc ctccacataa tcactcgcct tatagctggc
     4201 atacatcagc gcaggtacca gaaccagcgc ataagggccg gtggcacagg cggccataat
     4261 caggcaacct tctgcgatgg tcactatcgc ccccagtacg gcccctgcca gcgcccccaa
     4321 aaaactcttg tgctgaattt catcgcccag cctggcggcg ggggggccag acttttttga
     4381 tgccaacatc tgtgcaatgg ctttgtcgtt ggctgcccgt ttcatctggc tggcgaccat
     4441 cgcctgctgt gacagcattc gcattgccag cgtaccgcct gcggtcgctc cctgtggccc
     4501 gcctggtcct tcactcatgt gttatccctc gtttatgtca gcgcggctgg tccttttgga
     4561 tgtcattctc cggtggcaac ggggtgaagc tctttatcac ctccagcagc atcgtctttt
     4621 gactgttctt cagggataag gaggtgctga aagtgaaggt cagcagcgta tctccacgcc
     4681 gctggaatac ggctatctgg ctaatccgcc cctgattccc ctcatcttcc ggaccgttcc
     4741 aggaataatc cagcagccag gccggggcct ctgccagagt gatttcccgg caggcatgtt
     4801 cggtgtaccc atcaagtttg ccctggtaca gcgccagcgt ttcctggtac agctctggtg
     4861 tcagggttcc ctgtggaacg gcttcgcggg taatgaccag gttgatgcca ctgttatctg
     4921 tggacaccag gacctgcatt gactgatcct gccattgaga cggcagttga atgtggcctt
     4981 cattaaaagt gaactgcata aaatactccc tgataatctg agtgaatcag ttgatgaaaa
     5041 cagaaccgcc cacgatggtg atgccatcct tcgacagcct gattaccgtc gattccccga
     5101 cctgtagctg aatgctatcc ggcgtcagcg caattttgct ttcctccacg gccagcaaaa
     5161 tttccttaat cgccgtataa gcctgtatac cgtcgccttc gccaccattg tttacagcgt
     5221 caacatgcac cgaaccggca atcgtgcctc tgtcctgaaa gacatcctca gataaagacc
     5281 ctttcagtac tccggtgatc ttgtggcctt tttccaccgt aagaaggcgg ttaccttcct
     5341 ggatcgtgac cgtctggtca ttttttacgg tttcgcggtg atcgtgtttt acggtggtgg
     5401 tacggtcgtt gagcacttcg gtatccatgt tcttctgcgc gtggatgtag acctgttctc
     5461 tccctgtcgc atcatcaaac ttcagctcat taaatccgct gcccatgtac gttttggagc
     5521 ggatggtcat ctgcgtcttt gttcccggca ggctgccggg gggggcggtt ttcctggtgg
     5581 taggtgcgcc ccataatgat cggctggtcc ggatcgccgt tgaggaagtc tacaatcact
     5641 tcctgaccca cacgcggtat cgccaggtgg ccaaaaccgg tgcctgccca cgcctgtgcc
     5701 acacggatcc agcacgaact gtcttggtct gccgggttat aacggtccca gttgaacttc
     5761 acccgcacgc gaccatgttc gtcgcagaag atttcctcgc ctgccggtcc tgtcacgaca
     5821 gcgctctgcg ggccgtcgac cagcggtttc agcaacggct gtggtcgcca tgttctgtct
     5881 gccgggatca ccgcaaaatg gttctccagc gccgtccccg ctccctgccg tcctggcacc
     5941 gcctgtggct gttcgccgtg cagttcactt gccaccacct gccattcccg gttcaggttc
     6001 gcctgcggat gccccgtcag cacaattcgt cgtcccggcc atatctccgg cgagcgactc
     6061 attccccgcg cggtttctgc attgtttcgc cagccgtcca tctgccagcg ggcaaagttc
     6121 tgcccgtgag cacccttgaa acgtccgggg tagtcataca cttcatactg cgtgcgctgg
     6181 tagtcctggt gctggccttc ctgttcaaaa cgtccggccc agccggggcg tttaaaggtg
     6241 tagtctttgg tcaccacgga agaagggcgg atttgtgcgc tgtagcggaa ctggctgatg
     6301 cagagggtgc tcacctcggt acgggtgttc gggttccatg ggatttcaaa agattcgggc
     6361 agatggcgga ctgtgtcgca cagcaccagg ctctggtcgg tacttttgta agcatgctcc
     6421 tcataaaaga agatgccttc ctccgccgcc atccggcaca ggaaatcgta atcagtctca
     6481 ccgtactgga cacaaaactc acgggaagga tgcggctcgc tgaacagcgg actccattcg
     6541 gtcaccccgt tttcctgcaa catcgtgccg aggatgcttt tgatgtcctc gttctggaag
     6601 atacggaagt tctggcgcag accggcacgc cacagcggcg ggtgcacctt catgctgtac
     6661 agcatctggt ttttgtcgtt ctccccgagt tcaaaccagg tcaccacgcc tttcacccgg
     6721 cgctgtactt catcgccctg ccatatcgtc aggtaggcca ttttgtccag cacctgcgca
     6781 aattcaaggg agagaaactg ctggctgacc agggagagat cgagggaaaa gagcgaagag
     6841 agtgactggt tcagatgaaa ggaaaccacc gcaaaagcgt ccggcggcag gccgtccact
     6901 tccagtgtga aacgtaatcc ggttgacata gcaacctcca gtaaaatgaa taatgatccg
     6961 taccgtacaa ctgcggataa tgtgataagc gggacgactt cagacgttat tagggaagga
     7021 acagaaaatg ctatggttat gtgagaattt tgtttaaata ttactttaca gaaacgggtg
     7081 tctgttcagt caaaaaaata atcacaagct caacctcttg tttcttggcg ttattcagga
     7141 caggtacgca tatcacaacg agtaacaaaa acgcaaagtt aaaccaatat tcataatata
     7201 tttttattca cagcaataaa taatccaatt aacattatta aaatgaatga ttgcataaga
     7261 aatatatgct accgctccac
//
LOCUS       DAZPLI010000185         7172 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZPLI010000185 DAZPLI010000000
VERSION     DAZPLI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.186"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..502
                     /locus_tag="ACHUU3_003751"
     CDS             <1..502
                     /locus_tag="ACHUU3_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR2083719.1"
                     /translation="FSFKDTLLMRMNDLNNDLVNYSHDNTRTSVGARLAMYEVGLKTY
                     SPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDLIDTLSTRGIPGVVLTILAF
                     SAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFVTIILLCAYFKAQSD
                     QCLLDK"
     gene            complement(572..1543)
                     /gene="rfaC"
                     /locus_tag="ACHUU3_003752"
     CDS             complement(572..1543)
                     /gene="rfaC"
                     /locus_tag="ACHUU3_003752"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR2083720.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(1547..2593)
                     /gene="rfaF"
                     /locus_tag="ACHUU3_003753"
     CDS             complement(1547..2593)
                     /gene="rfaF"
                     /locus_tag="ACHUU3_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR2083721.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(2603..3535)
                     /gene="rfaD"
                     /locus_tag="ACHUU3_003754"
     CDS             complement(2603..3535)
                     /gene="rfaD"
                     /locus_tag="ACHUU3_003754"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR2083722.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            3749..4945
                     /gene="kbl"
                     /locus_tag="ACHUU3_003755"
     CDS             3749..4945
                     /gene="kbl"
                     /locus_tag="ACHUU3_003755"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR2083723.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            4955..5980
                     /gene="tdh"
                     /locus_tag="ACHUU3_003756"
     CDS             4955..5980
                     /gene="tdh"
                     /locus_tag="ACHUU3_003756"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR2083724.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            6219..7121
                     /locus_tag="ACHUU3_003757"
     CDS             6219..7121
                     /locus_tag="ACHUU3_003757"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR2083725.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMW"
BASE COUNT         1759 a         1738 c         1804 g         1871 t
ORIGIN      
        1 attttctttt aaagatacat tgcttatgag aatgaatgac ttaaataacg atttagttaa
       61 ttattcgcat gataacacca gaacttcagt cggtgcccgt ctggcaatgt atgaagttgg
      121 cttaaaaaca tattctccaa taggacaatc actggaaaaa cgagcggaaa aaatacatga
      181 actagaagaa aaagagccta gattgagtgg cgctttaccc tatatagatt ctcatttgca
      241 taacgatctc atagatacgt tatcaacgcg tggtattcct ggagttgtat taacaatttt
      301 agccttttca gcaatactca tatatgcctt aagaactgct aaagaacctt atattttaat
      361 cttgcttttt tcactactgg tagtaggcct aagtgatgta atactctttt ctaaaccggt
      421 tccgactgct gtgtttgtca ccataatatt gctttgtgct tattttaaag cacaatcaga
      481 ccaatgttta ttagataaat aatgaatatg tgaaataaaa tcagcaagga ggctgatttt
      541 atttcaaaat tatctattaa ggggttgtaa tttaaatcat agcagcgttt tcttcaatga
      601 atcgcttaac agcatttgct gtcaattgag acaactcatt ccccggagcc ctacaaacca
      661 tctgattctt cccatatcca ccaattaatc ccggatcggt tggtccataa accgtgatat
      721 tgggtctatc cagtgccgcc gttaaatggc ttaaccccgt atccaccgac actacaaatt
      781 tagccccggc cagcacgcgg gcaacgcctt ccagactcat cttcggcaat acttcaacat
      841 aagcaaatcc ttccgccagt cgtttcgccc gctcttcctc atgcggcgcg ccccacggaa
      901 gtttaatccg tattcctgaa tcagccagta aaccaatcaa ttctcgccag tgttcttccg
      961 gccagtgttt atcatcacgg gtcgtcgcat gaagaaatac ggcatattcg ccagcatctg
     1021 taggcaggtt cgtcaggaaa tgctgtgcaa tagcataatc gccctgggtt tgcggtttgc
     1081 tatagcccag acttttggca aacagttcgc gggtgcgttc tacggcgtgc tgctgttttg
     1141 caatatgatg cttacgattg taaaacagac tggctaaagg ttcgcgagcg gtttgccagt
     1201 ccatgccatg ctttacgcca tgcgccagac gcgtcaccag cgccgcgctt tttaccagcc
     1261 cctgagcgtc gataactgcg tcatagttct ctgcttgtag cgcttcacga aacgctttgc
     1321 gttccgcttt tatgggggcc gagaaccagg ctttacgcca gcgacgtatt gccacaggaa
     1381 taactcgctc aacggcggcg tgccaggaag gaatctgtgc gaacccttct tccaccaccc
     1441 agtcaaactt aatccctggg attgcctgct gggcatcagt gagtgcgggc aacgtatgga
     1501 gaacatcgcc catcgacgat gttttaacga tcaaaacccg catccgtcag gcttcctctt
     1561 gtaacaatag cgcgttgagt tcttccagta cgcgctgggg agtaatgtcg attaagctct
     1621 ggtgataacc ctccgcagcg tcacctttac gcactttgtg atagccggta atcaggcgga
     1681 tcacgcgcgc tttatgggat agcggcggtg tgaagtccgg gctactcgga ccatacaggg
     1741 caaccagcgg acgattgagc gccgccgcaa cgtgcatcag gccagaatcg ttagtgacaa
     1801 tggctttaca ggctgcaatc aggataaccg cttgatcaag ctgtgtttcc cccgccaggt
     1861 tccgacacca tgcctgctgc tcggtattca aagcggcaag aatctcattg cccgcttcat
     1921 gatctttcgc cgagccaaac agaaccacct gataaccttc atcaatcagc tgctttgcca
     1981 gttccgcata gtggtagtgt ggccagcgtt ttgccggacc aaactccgca cccgggcaaa
     2041 aaccaatcat cggacgttct gatgaaagcg aaaattgatt acaggtatat gatttttcac
     2101 cttcgctcac ctgcaattgc ggccataaca acggttttgg tagatcttgt gcggtacgca
     2161 tcacgccttt gtcataggcc agcgcgacat agcgttccac cattagcggc caggcttctt
     2221 tatcgagcac gcgtaaatcg ttgagtaaac cgtagcgcat ctcgccgcgc cagccggtgc
     2281 gatgaggaat acccgcgaag aaaggcacta atgcagattt gaaggagttg ggtaagacgt
     2341 aggcgcggtc gtagcgcttt tcacgcaggc tatgacccag tttgcggcgt tcgccgattt
     2401 ccagcgctcc gtgaccgaga ggcataggaa tagcttcgtt aacttccggc atccgcgata
     2461 ataatggacg gcaccatgcc ggtgccatca catcgattat cgcctgggga tagcgcgcct
     2521 ggagcgtgcg atagagactt tgcgacatca tcatgtcgcc aacccaagac gggccgatca
     2581 ccagtatttt catgcagagc tcttatgcgt cgcgattcag ccaggccatg tattccgtta
     2641 caccttcagc aacggttttg aacggtttgt cgtaacccgc cgcgcgcaga tttgtcagat
     2701 ctgcctgagt gaacgcctgg tagcggcctt tcagcttatc cgggaacgga atgtattcga
     2761 tctggccttt cttgtgataa gccagcgtag catcagctac agcctggaag gattccgcac
     2821 gaccagtacc gaggttgaag atgccggaaa cgccattttc caggaaccac agattcacat
     2881 cagccacgtc gcccacatag acgaagtcgc gtttgaagtt ctcgctacct tcaaacagct
     2941 tcggtgattc accgttgtta agctgggtgt tgagatggaa agcgacgctc gccatgctgc
     3001 ctttatggcc ttcacgcggt ccataaacgt tgaaatagcg gaagccaaca atctgcgagt
     3061 tagcttcagg caggatttgg cgaacatatt catcaaacag gaattttgag taaccgtaga
     3121 cgttcaacgg tttttcgtac tcgcgggatt caataaagtc ggaggtgcgt ccgccgtagg
     3181 tggctgcgga agaggcatac aggaacggga tttcgcgctc cagacagtag tgcagcagct
     3241 ctttggagta ttgatagttg ttatccatca tatacttgcc atcccactcg gtggtggaag
     3301 agcacgcgcc ttcgtggaaa atcgcttcga catcgccgaa ctcttcgcca gccataatct
     3361 ggatcaggaa gtcttcctta tccatatagt ccgcgatatc cagatccacc aggttcacaa
     3421 acttggtgcc gtctttcagg ttgtccacca ccagaatatc ggtgatgcct ttatcattca
     3481 gggccttaac gatgttgctg ccgataaagc ccgcgccgcc ggtaacgatg atcataactg
     3541 taaccttcga attatggagt cagagacaat ctcagacacg aatacttcta tcatatcact
     3601 acacggcgtt ggcttcagcc attcgccaaa agtgagattg atggacgtta tttatcctga
     3661 attttgcaga agtgttaacg cgtcatctcg tcgcgaccta taagtttggg taatatgtgc
     3721 tggaatttgc cctgtctgga gaatcgcaat gcgtggagaa ttttatcagc agttaaccaa
     3781 cgatctggaa accgcacggg cggaagggtt gtttaaagaa gagcgcatta ttacgtctgc
     3841 gcagcaagca gatatcactg tggctgatgg aagccacgtc attaactttt gtgccaataa
     3901 ctatctcggg ctggcgaatt atccggatct gattgcggcg gcaaaggcgg gaatggattc
     3961 tcacggtttc ggcatggctt cggtgcgttt tatttgcggc actcaggaca gccataaaga
     4021 gcttgaacaa aaactggcgg cattcctggg gatggaagat gcgattctct actcttcctg
     4081 ctttgatgct aacggtggcc tgtttgaaac gctgctgggc gcggaagacg ccattatctc
     4141 cgacgcactg aaccacgcgt ctattattga tggtgtgcgt ctgtgtaaag ctaaacgcta
     4201 tcgctatgcc aacaacgata tgcaggagct ggaagcacgt ctgaaagaag cgcgtgaagc
     4261 cggtgcgcgt catgtgctga ttgccaccga tggtgtgttc tcgatggacg gcgtgattgc
     4321 caacctgaag ggcgtttgcg atctcgcaga taaatatgat gccctggtga tggtagacga
     4381 ctcccacgcg gtcggttttg tcggtgaaaa tggtcgtggt tcccatgaat actgcgatgt
     4441 gatgggccgg gtcgatatta tcaccggcac gctcggtaaa gcgctgggcg gggcttctgg
     4501 tggttatacc gcggcgcgca aagaagtggt tgagtggctg cgccagcgtt ctcgtccgta
     4561 cctgttttcc aactcgctgg caccggccat tgttgccgca tccatcaaag tactggagat
     4621 ggtcgaagcg ggtagcgagc tgcgtgaccg tctgtgggcg aacgcgcgtc agttccgtga
     4681 gcaaatgtcg gcggcgggct ttaccctggc gggagccgat cacgccatta ttccggtcat
     4741 gcttggtgat gcggtagtgg cgcagaaatt tgcccgtgag ctgcaaaaag agggcattta
     4801 cgtcaccggt ttcttctatc cggtcgttcc gaaaggtcag gcgcgtattc gtacccagat
     4861 gtctgcggcg catacccctg agcaaattac gcgtgcagta gaagcattca cgcgtattgg
     4921 taaacaactg ggcgttattg cctgaggatg tgagatgaaa gcgttatcca aactgaaagc
     4981 ggaagagggc atctggatga ccgacgttcc tgtaccggaa ctcgggcata acgatctgct
     5041 gattaaaatc cgtaaaacag ccatctgcgg gactgacgtt cacatctata actgggatga
     5101 gtggtcgcaa aaaaccatcc cggtgccgat ggtcgtgggc catgaatatg tcggtgaagt
     5161 ggtaggtatt ggtcaggaag tgaagggctt caagattggc gatcgcgttt ctggcgaagg
     5221 ccatatcacc tgcggtcact gtcgcaactg tcgtggcggt cgtacccatc tgtgccgcaa
     5281 cacgatcggc gtcggcgtta accgcccggg ctgcttcgcc gaatatctgg tgattccggc
     5341 gttcaacgcc ttcaaaattc ccgacaatat ctccgacgac ctggcttcca tttttgatcc
     5401 cttcggtaac gccgtgcata ccgcgctgtc gttcgatctg gttggcgagg atgtgctggt
     5461 ctctggtgca ggcccgattg gtattatggc agcggcggtg gcgaaacacg ttggtgcacg
     5521 caatgtggtg atcactgatg ttaacgaata tcgtctcgaa ctggcgcgca aaatgggtat
     5581 cacccgtgcg gtcaacgtcg ctaaagaaaa ccttaatgat gtgatggcag aactgggcat
     5641 gaccgaaggc tttgatgtcg gtctggaaat gtccggtgcg ccgccagcgt ttcgtaccat
     5701 gctcgacacc atgaaccacg gcggtcgcat tgcgatgctg ggtattccac cgtccgatat
     5761 gtctatcgac tggaccaaag taatctttaa aggcttgttc attaaaggta tttacggtcg
     5821 tgagatgttt gaaacctggt acaagatggc ggcgctgatt cagtcaggtc tggatctctc
     5881 gccgatcatt acccatcgtt tctctatcga tgatttccag aagggctttg acgctatgcg
     5941 ttcgggccag tccgggaaag taattctgag ctgggattaa cacgaacaag ggctggtatt
     6001 ccagcccttt tgcctgagga taatccgtta aatatgtaaa atcctgtcag tgtaataaag
     6061 atatcgtaat tgcgctgatc tcttatatag ctgctctcat tatctctcta ccctgaagtg
     6121 actctctcac ctgtaaaaat aatatctcac aggcttaata gtttcttaat acaaagcctg
     6181 taaaacgtca ggataacttc agaggtcgtc ggtaatttat gatgaacagc accaataaac
     6241 ttagtgttat tattccgtta tataatgcgg gcgatgattt ccgcacttgt atggaatctt
     6301 taattacgca aacctggact gctctggaaa tcattattat taacgatggt tcaacggata
     6361 attctgttga aatagcaaag cattacgcag aaaactatcc gcacgttcgt ttgttgcatc
     6421 aggcgaatgc tggcgcatcg gtggcgcgta atcgtgggat tgaagtggca acgggcaaat
     6481 atgtcgcttt tgtcgatgct gacgatgaag tctatcccac catgtacgaa acgctgatga
     6541 ccatggcgtt agaggacgac ctcgacgtgg cgcagtgcaa cgctgactgg tgttttcgtg
     6601 aaacgggaga aacctggaaa tccatcccca gcgatcgcct tcgctcaacc ggcgtattaa
     6661 ccggcccgga ctggctgcgg atggggcttt cttcgcgccg ttggactcac gttgtctgga
     6721 tgggggttta tcgccgcgat gttattgtta aaaataacat taaatttatt gccggattac
     6781 atcatcagga tattgtctgg acaacagaat ttatgtttaa tgcgctgcgt gcgcgatata
     6841 ccgagcaatc gttatataaa tattatctgc ataatacgtc agtgagtcgg ttgcatagac
     6901 aagggaacaa aaaccttaat tatcaacgtc actatattaa gattactcgt ctgctagaga
     6961 aattaaatcg aaattatgcc gacaaaatta cgatttatcc ggaatttcat cagcaaataa
     7021 cttacgaagc attgcgtgtt tgccatgcgg tgcgcaaaga gccggatatt attacccgcc
     7081 aacggatgat tgccgagata tttacttccg gtatgtggta atgactccaa cttattgata
     7141 gtgttttatg ttcagataat gcccgatgac tt
//
LOCUS       DAZPLI010000186         7057 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZPLI010000186 DAZPLI010000000
VERSION     DAZPLI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7057
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.187"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            108..605
                     /gene="ftnA"
                     /locus_tag="ACHUU3_003758"
     CDS             108..605
                     /gene="ftnA"
                     /locus_tag="ACHUU3_003758"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR2083726.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(643..882)
                     /locus_tag="ACHUU3_003759"
     CDS             complement(643..882)
                     /locus_tag="ACHUU3_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR2083727.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            1073..2284
                     /gene="tyrP"
                     /locus_tag="ACHUU3_003760"
     CDS             1073..2284
                     /gene="tyrP"
                     /locus_tag="ACHUU3_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR2083728.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2346..3011)
                     /gene="yecA"
                     /locus_tag="ACHUU3_003761"
     CDS             complement(2346..3011)
                     /gene="yecA"
                     /locus_tag="ACHUU3_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR2083729.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3207..3293)
                     /locus_tag="ACHUU3_003762"
     tRNA            complement(3207..3293)
                     /locus_tag="ACHUU3_003762"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3257..3259),aa:Leu,seq:taa)
     gene            complement(3306..3379)
                     /locus_tag="ACHUU3_003763"
     tRNA            complement(3306..3379)
                     /locus_tag="ACHUU3_003763"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3345..3347),aa:Cys,seq:gca)
     gene            complement(3434..3509)
                     /locus_tag="ACHUU3_003764"
     tRNA            complement(3434..3509)
                     /locus_tag="ACHUU3_003764"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3474..3476),aa:Gly,seq:gcc)
     gene            complement(3661..4209)
                     /gene="pgsA"
                     /locus_tag="ACHUU3_003765"
     CDS             complement(3661..4209)
                     /gene="pgsA"
                     /locus_tag="ACHUU3_003765"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR2083730.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4266..6098)
                     /gene="uvrC"
                     /locus_tag="ACHUU3_003766"
     CDS             complement(4266..6098)
                     /gene="uvrC"
                     /locus_tag="ACHUU3_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR2083731.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6095..6751)
                     /gene="uvrY"
                     /locus_tag="ACHUU3_003767"
     CDS             complement(6095..6751)
                     /gene="uvrY"
                     /locus_tag="ACHUU3_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR2083732.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         1721 a         1813 c         1667 g         1856 t
ORIGIN      
        1 attcgtattt ttaataattg aagtttatat tttacctttt gcaaataata aaataacaaa
       61 ttatagtgac gccacagcaa caaatataac ctttgtggag cactatcatg ctgaaaccag
      121 aaatgattga aaaacttaat gagcagatga acctggaact gtactcttca ctgctttatc
      181 agcaaatgag cgcctggtgc agctatcata ccttcgaagg tgctgccgcg ttcctgcgcc
      241 gtcacgccca ggaagagatg acgcatatgc agcgtctgtt tgattacctg actgataccg
      301 gcaatttacc gcgtattaat accgttgaat ctccgtttgc tgaatattcc tcacttgatg
      361 aattattcca ggaaacctat aaacacgaac aattaatcac ccagaaaatt aacgaactgg
      421 ctcatgctgc aatgaccaat caggactacc caacatttaa tttcctgcag tggtatgttt
      481 ctgagcagca tgaagaagag aaactgttca aatcgattat tgataaatta agcctggcag
      541 gcaaaagcgg cgaaggtctg tattttatcg acaaagaact ctctaccctc gacacacaaa
      601 actaatgctc tccggcggca ggtttactgc cgccgtttca aatcagtgac ggcaaatctt
      661 actctgatcg gtcgaaaaac cgtctttcga aggtataaat ttgccttttg ctgccagaaa
      721 cgctaccagc tctcccgctg tcatcccttc tgccgagcag gtgtgaaaac gtgcctgttc
      781 accaaaacgc gctttaatcg cagcttccag actggcatgc gtatattgct cgcctgattc
      841 aatcatcata tttaacactt catgaccgtg aatagagtcc atcgttcctc ctcaaaaaaa
      901 gcctagcgta gcgattgccg cttatgaaga ctttgcgcca gcgcaggact gaatgctttt
      961 tattgtacat ttatatttac actatatgta acgtcggttt gacgaagcag ccgttatgcc
     1021 ttaacctgcg ccgcagatat cactcataaa gatcgtcagg acagaagaaa gcgtgaaaaa
     1081 cagaaccctg ggaagtgttt ttatcgtggc gggaaccaca attggcgcag gcatgctggc
     1141 aatgccgctg gctgcggccg gtgttggttt tagcgttacg ttaatcttgt tgattgggct
     1201 ttgggcgttg atgtgctaca cggcgctatt actgctggag gtgtaccagc atgttccggc
     1261 agataccggt ctgggcacgc tggcaaaacg ctatctggga cgctacggtc aatggctgac
     1321 gggcttcagt atgatgttct taatgtatgc tctgactgcg gcatacatca gcggtgtcgg
     1381 tgaattgttg gcctccagca tcagcgactg gacaggtatt tcaatgtcgg caaccgctgg
     1441 cgtgctgttg ttcacttttg ttgccggtgg cgtggtttgt gttggaacat cgctggtcga
     1501 tttatttaac cgttttctgt tcagcgccaa aattattttt ctggtggtga tgctggtatt
     1561 gctgctaccg catattcaca aagtgaatct tttaaccctg ccgttgcaac aggggctggc
     1621 tctgtcagca atcccggtga tttttacctc atttggtttt cacggtagcg tgccgagtat
     1681 tgtcagctat atggatggca acgttcgtaa gctacgctgg gtgtttataa ccggtagtgc
     1741 gatccccctg gtggcatata ttttctggca ggtggcaacg cttggcagca ttgattcaac
     1801 aacctttatg ggattgctgg ctaatcatgc tggattaaac gggctgttac aggcgttacg
     1861 cgaaatggtg gcctctccgc atgttgagct ggcagtgcat ttatttgctg atttagccct
     1921 cgccacgtca tttctcggcg ttgcgttagg cttatttgat tatctggctg atttatttca
     1981 gcgttcaaat accgttggtg gacggttgca aactggtgca attacctttc tgccgccgtt
     2041 ggcgtttgca ctgttttatc cacgaggatt tgtgatggcg ctgggttacg ccggtgtggc
     2101 gctggcggta ctggcattga ttatcccttc gctgttgacc tggcaaagca gaaagcacaa
     2161 tcctcaggcg ggttaccggg tcaaaggtgg tcgtccggcg ctggtggtgg tgtttctctg
     2221 tggtattgct gtgattggcg tgcaattttt gattgcggca gggttgttac cagaagtggg
     2281 gtgatcagat agcctcaaat tccttattgg gtgccagaat taacgctgac acccaatttg
     2341 gcctcttaat gcaggcagca ctgcttaaat ttcttaccac taccgcacgg gcaaggatcg
     2401 ttacgccccg gtttctcttc tgctttgatc ggttgctgaa cagctttttc ctgcggatgc
     2461 gccatccagt acgcatgtag atcaagcgcc gccagtcgaa tggcatctac gctctcttca
     2521 aacgcttctg gcgacatctt ttctacccgc tcgaagtttt cctcagtacc gtgcagcgca
     2581 atcgcctcca gcgctggttt taacgaatcg ggcaacgttg accagtcaga aagtgccacg
     2641 ccccgcatat agccaaagca ccactcctca acaatcgtca gctcgctgcc atcaacttct
     2701 cgcaagccga ataacggctc aaactgctcc gggaattcgt tcagacgctc tgcggtatcg
     2761 gccatatgtt gaaaagccag gttcataaag cgcgtcatct ctttctctga cgcccagcgc
     2821 ggcacatagt cagccccacc ccacacggca accagccact gttccggttc aatctcttgc
     2881 ggagaactca acaccgccgt caataaaccg tccagctccg ccacatcaag gatggcgtgg
     2941 ttagtgttgt atttggtcag aatatcgtcc agccattcca actcactttc gtttaacggt
     3001 cccgttttca tacgcttttc cttgtggatc tcaactcgcc agcacctatc ttacatgccg
     3061 gtccgtatca gagatacttt ttgagtggct ttgccggtga ttaaaaatta aggagggtgt
     3121 aacgacaagt tgcaggcaca aaaaaaccac ccgaaggtgg tttcacgaca ctgcttattg
     3181 ctttgatttt attcttatct ttcccatggt acccggagcg ggacttgaac ccgcacagcg
     3241 cgaacgccga gggattttaa atcccttgtg tctaccgatt ccaccatccg ggctcgggaa
     3301 gaaagtggag gcgcgttccg gagtcgaacc ggactagacg gatttgcaat ccgctacata
     3361 accgctttgt taacgcgcca aattcttcag gcctttcagc cagacatccg cttgacgccg
     3421 atgtctttta aactggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg
     3481 tcgtgctcta ccaactgagc tattcccgca ttcatcaagc aatcagttaa tcacttgatt
     3541 ttattatcgt ctggcaatca gtgccgccgt tcgatgcgtt gcattctact tacctggcgc
     3601 gatgagtcaa cgatattttt caccactttt gatcgtttgc tgaaaattac gccgaaacga
     3661 tcactgatca agcaaatctg cacgcgcagc gctcaaatat tgcaacattg accacagagt
     3721 cagtaccgca gccacaaaga aaagtgcaat accggcgtac tcaacccaaa tgttcggacg
     3781 ccacagcagc catgccaacg ccaccatctg ggcagtggtt ttcactttcc caatccagga
     3841 gacagccacg ctactgcgtt tacccaactc cgccatccat tcgcgtagcg cagaaataat
     3901 aatttcacgg gcgatcatcg ttgctgccgg taaggtcacc caccagctgt ggtaatgctc
     3961 ggttaccagc accatggcga tagccacgag aactttatct gctacagggt caaggaaagc
     4021 accaaaccgg gtactctggt tccagcggcg tgccagaaaa ccatcgaacc agtcagtcac
     4081 cgccgcgacg cagaaaatga gcgcggcggc aaacggcgac caggtgacag gcagataaaa
     4141 gaccaataca aagaatggga taaggatgac acggaacagt gtaagcaacg tagggatatt
     4201 aaattgcata atgacgggta actatctgtt gtcagtaaga ttacccctat gttgctacag
     4261 agacatcaat gtttcaacga ccagaagatc ttttctgcca gaccttgcga aatacccggc
     4321 acttttgcaa tttcctcgac gctggcgtta cgtaaacctt gcaaaccgcc catatatttc
     4381 aacaacattt gccgacgttt tggcccgacg ccttcaatgg tttccaggga actggtattt
     4441 ttgaccttcg cccgtttttt acggtgcccg ccaatcgcgt gatcatgtga ttcatcgcga
     4501 atatgctgga taacatgcag cgcgggagaa tctggcggca aactgaatcc ctcaccttcc
     4561 ggctcaaaga acagcgtttc cagcccagcc ttacgatctg ctcctttggc aacgccaagt
     4621 agcagcggat gatttttatc ccatgagaca tccagttcgg cgaagacatt tttcgcctgc
     4681 gcaagctggc ctttgccgcc gtcgataagg atcacatccg ggatcttact gtcgtcaatg
     4741 gctttaccat aacgccgacg cagcacctga ttcatcgccg cataatcatc gcccggcgtg
     4801 atgcctgtaa tgttatagcg ccgatactcc gcacgcagcg ggccgttagc atcaaacacc
     4861 acacaggaag cgacggtttg ttcgcccatg gtatggctga tgtcaaagca ctccatccgc
     4921 ttcacttccg gcaatttcaa cacgctggca agcgcggtca gtcgctggtg aacggtagat
     4981 tgctgcgaaa gtttgctggt taaggccgtc gccgcattgg tgcgcgcgag tttcagataa
     5041 cgcgccctat cgccgcgagg tttggtttga acattaatct tgcgtcccgc cagttctgaa
     5101 agggaatcgg cgagcagcgt tttatcgcta agattaaaat cgagcaggat ctcacccggt
     5161 aaggtgcgca tctggctgcc ttgtaaatag aactggccta cgaaggtttc taccacctcg
     5221 ctcagttccg taccgccagg cactttcggg aaatagctgc ggctgccgag cactttgccc
     5281 tgacgaatga acaatacgtg gacacaagcc atgcccgcat cgaacgccac accaataacg
     5341 tcgaggtcgt cgccggtatt ggaaacgaat tgtttttcgg tgacgcgtcg caccgcctga
     5401 atttggtcgc gaatacgtgc agcttcttca aactccagat tctggctggc agtttccata
     5461 cgactaatga gttgcgtaag cacctgatca tctttgccag acaaaaacag gcgcacatac
     5521 tcaacctgct gagcgtattc ttcttcactc accagtcctt caacgcacgg tcccagacag
     5581 cgccctatct gatattgcag acacggacgc gagcgattgc gataaacact attttcgcac
     5641 tggcgaatgg ggaaaatctt ttgcagtagc gccagtgttt cacgtacggc atagccattc
     5701 gggaacgggc cgaaatattc acctttggca tgcttcgcac cacgatgcat cgccagacgc
     5761 gggtgggtat cgccactcag gaagataaaa ggataagatt tatcatcgcg tagcaaaacg
     5821 ttgtaacgcg gctgatagag tttgatgtag ttgtgttcca gcaacagcgc ttcggtttct
     5881 gtatgcgtaa ccgttacatc aatttgctgg atctgggcga ccagcgcttc ggttttgcgc
     5941 gaagcgaggt tgctgcggaa atagctggaa agccgttttt tcaggtcttt cgctttgccg
     6001 acatagataa ccgtaccacc agcatcgtac atgcgataaa cgcctggctg gctggttacg
     6061 gtttttaaaa acgcttttgc gtcaaactga tcactcactg acttgataat gtctccgcat
     6121 tacacagacc atggcgaatt gccaggtgag tcagctcaac atcgccatga atgtttagtt
     6181 tactgaacat acgatagcgg tagctgttca ccgttttcgg actgagattg agctgttctg
     6241 agatctcatt gaccttctgg cccttggtga tcatcagcat aatctgcaat tcacgttcag
     6301 acaaactggc aaatgggctt tctgtttttt ctggttcgat ctggcttaac gccatttgtt
     6361 gagcgatatc agaagcaatg taacgctgcc ctgaatagac agaacgaatc gcactcacga
     6421 cttcctgcgg agccgcgcct ttgctgaggt agcccgcagc accggcctgc atgactttcg
     6481 ctggtaaagg gttttctgta tggacggtaa gcatgatgat tttgacatca gctgtggaac
     6541 gcgcgatttt acgcgtcgcc tcaagaccgc caatgcccgg catactcatg tccattagca
     6601 ccacgtcaac ggcatttgtc cggcaccact taacggcgtc ttcaccgcac gatgcctcac
     6661 cgacgacttt tatacccttt atatcttcca gaatgcgtcg tatccctgcg cgcaccagtt
     6721 cgtggtcatc aacaagtaga acgttgatca aaggaatatc tccagaaata gggataacgc
     6781 tactgatagt tagtcattcg tatattagcg gtttttattg caactttgaa acgttaaaaa
     6841 tggtgggtat tcgatttttc tctcgttttt gtaaattcga ttgtccacag ttatggtcac
     6901 gcccgtcacc aacggcttcc gggatcgttt cacagtaagc ccggcgatga aatgtgacaa
     6961 aaattgactt tattcagcaa aaatgaaaat cagcccgctg aatatgtaac attaattaac
     7021 ccgaacaata cgggcaaaaa acattcatga taaaaat
//
LOCUS       DAZPLI010000187         7020 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZPLI010000187 DAZPLI010000000
VERSION     DAZPLI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7020
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.188"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..815)
                     /locus_tag="ACHUU3_003768"
     CDS             complement(<1..815)
                     /locus_tag="ACHUU3_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR2083733.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACHUU3_003769"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACHUU3_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACHUU3_003770"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACHUU3_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1397..1873)
                     /locus_tag="ACHUU3_003771"
     CDS             complement(1397..1873)
                     /locus_tag="ACHUU3_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR2083734.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="ACHUU3_003772"
     CDS             complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="ACHUU3_003772"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR2083735.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3308..4348
                     /gene="bioB"
                     /locus_tag="ACHUU3_003773"
     CDS             3308..4348
                     /gene="bioB"
                     /locus_tag="ACHUU3_003773"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR2083736.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4345..5499
                     /gene="bioF"
                     /locus_tag="ACHUU3_003774"
     CDS             4345..5499
                     /gene="bioF"
                     /locus_tag="ACHUU3_003774"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR2083737.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5486..6241
                     /gene="bioC"
                     /locus_tag="ACHUU3_003775"
     CDS             5486..6241
                     /gene="bioC"
                     /locus_tag="ACHUU3_003775"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR2083738.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6234..6902
                     /gene="bioD"
                     /locus_tag="ACHUU3_003776"
     CDS             6234..6902
                     /gene="bioD"
                     /locus_tag="ACHUU3_003776"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR2083739.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENAATGKYIN
                     LALL"
BASE COUNT         1702 a         1753 c         1948 g         1617 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg
     1321 gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg
     1381 ccatctggca gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga
     1441 aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta
     1501 tatccagcgc gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc
     1561 catcgtaccc ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta
     1621 ccagaccaga gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta
     1681 cccagtgcca ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac
     1741 ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt
     1801 aacccatgcc gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac
     1861 tgatgagttt catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg
     1921 cggacttctc gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg
     1981 gtcagacgct gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg
     2041 ccaaaaggcc ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc
     2101 accggatgag tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt
     2161 tcggcatcac gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt
     2221 tgctgccagt cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc
     2281 ggattgccca taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt
     2341 tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct
     2401 ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg
     2461 gtacgaccaa atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat
     2521 attttgcgga ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg
     2581 acaatcggct caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca
     2641 aagcccacca tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca
     2701 aacaagtttt ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac
     2761 accgacatcg cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg
     2821 cgcgcttcgc ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct
     2881 acggaaccgg agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt
     2941 ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg
     3001 gcatcaattt gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc
     3061 gcccaccagg acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa
     3121 ccttcggcgc tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc
     3181 cagatatggc gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa
     3241 ttgaaaagat ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag
     3301 aagccccatg gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa
     3361 accgttgctg gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg
     3421 tcaggtccag gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa
     3481 atactgcccg caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt
     3541 tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat
     3601 gggcgcggcg tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca
     3661 gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca
     3721 ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc
     3781 ggagttttac ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga
     3841 aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac
     3901 ggtaaaagat cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag
     3961 cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt
     4021 cgatgccttt gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta
     4081 cgtgcgcctt tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat
     4141 ggctggcgca aactcgattt tctacggttg caaactgctg accacgccga atccggaaga
     4201 agataaagac ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct
     4261 ggcaggggat aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga
     4321 cgaatattac aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat
     4381 gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc
     4441 tggctggtgg cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta
     4501 agccatcatc cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt
     4561 agcggcggtt ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa
     4621 ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat
     4681 caggcagtca ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc
     4741 catgcctcct tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat
     4801 aacgatgtcg ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg
     4861 gtgacagaag gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag
     4921 gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc
     4981 ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg
     5041 acttttggca aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg
     5101 gattatctgc tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag
     5161 gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc
     5221 gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag tacaggattt gccgtttacg
     5281 cttgctgatt catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta
     5341 caactggcag aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc
     5401 gtacccgctg gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat
     5461 atcgaccgtc tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc
     5521 atttggtcgg gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga
     5581 cgtcttactg gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg
     5641 gccaggctgg atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct
     5701 ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg
     5761 agatatcgaa tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc
     5821 ggtgcagtgg tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc
     5881 cggaggcgtg gtggcgttta ccacgctggt gcagggatcg ttacccgaac tgcatcaggc
     5941 gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga
     6001 acagtcgctg aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga
     6061 tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg
     6121 gcgcgacccg cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca
     6181 acaacagggg cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta
     6241 aacgttattt tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac
     6301 ttttacaagc cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg
     6361 gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca
     6421 gcagcctgca gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc
     6481 cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat
     6541 tacgcgcgct tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta
     6601 cgccgctttc tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga
     6661 tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa
     6721 tacaacacgc cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa
     6781 aacgtcacgc tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag
     6841 agatcccctg gcttgcagaa aatgcggcaa ccggaaagta cataaacctt gccttgttgt
     6901 agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga ctgcaattta
     6961 accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat gcggcgaatg
//
LOCUS       DAZPLI010000188         6998 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZPLI010000188 DAZPLI010000000
VERSION     DAZPLI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.189"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            503..1492
                     /gene="araF"
                     /locus_tag="ACHUU3_003777"
     CDS             503..1492
                     /gene="araF"
                     /locus_tag="ACHUU3_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFR2083740.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1562..3076
                     /gene="araG"
                     /locus_tag="ACHUU3_003778"
     CDS             1562..3076
                     /gene="araG"
                     /locus_tag="ACHUU3_003778"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR2083741.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3091..4077
                     /gene="araH"
                     /locus_tag="ACHUU3_003779"
     CDS             3091..4077
                     /gene="araH"
                     /locus_tag="ACHUU3_003779"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR2083742.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4244..5044
                     /gene="otsB"
                     /locus_tag="ACHUU3_003780"
     CDS             4244..5044
                     /gene="otsB"
                     /locus_tag="ACHUU3_003780"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR2083743.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5019..6443
                     /gene="otsA"
                     /locus_tag="ACHUU3_003781"
     CDS             5019..6443
                     /gene="otsA"
                     /locus_tag="ACHUU3_003781"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFR2083744.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6450..6878)
                     /gene="uspC"
                     /locus_tag="ACHUU3_003782"
     CDS             complement(6450..6878)
                     /gene="uspC"
                     /locus_tag="ACHUU3_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFR2083745.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1770 a         1629 c         1817 g         1782 t
ORIGIN      
        1 atgattttat aaaacgtttt ttcatttttg cgagatggct ctcattatac gtgttctgtt
       61 aattaatgag cacagtgata attaatggcg aaatgaatga cgtgcatttt ccacatcttt
      121 gagttgcggt tattacacca tttcaaaaac tcaacgccag gtaatgcggc ctattgactg
      181 gttaaaaaga agacatcccg catgggtacc aaagacaaca aggatttcca ggctaatctt
      241 atggattaat ctgctgtgca ttcgacaatt tgtctgacaa attggctttc ccttatgtct
      301 tttcccgcta aatttatgca cgttctcact gtaattctgc gatgtgatat tgctctccta
      361 tggagaatta atttctcgct aaaactatgt caacacggtc acttatcttt tagataaaag
      421 gtaatgcttt gttttccgat taatttaacg aaagtcattc gtttttgccc tacacaaaac
      481 gacactaaag ctggagagaa ccatgcacaa atttactaaa gccctggcag ccattggtct
      541 ggcagccgtt atgtcacaat ccgctatggc ggagaacctg aagctcggtt ttctggtgaa
      601 gcaaccggaa gagccgtggt tccagaccga atggaagttt gccgataaag ccgggaagga
      661 tttagggttt gaggttatta agattgccgt gccggatggc gaaaaaacat tgaacgcgat
      721 cgacagcctg gctgccagtg gcgcaaaagg tttcgttatt tgtactccgg accccaaact
      781 cggctctgcc atcgtcgcga aagcgcgtgg ctacgatatg aaagtcattg ccgtggatga
      841 ccagtttgtt aacgccaaag gtaagccaat ggataccgtt ccgctggtga tgatggcggc
      901 gactaaaatt ggcgaacgtc agggtcagga actgtataaa gagatgcaga aacgtggctg
      961 ggatgtcaaa gaaagcgcgg tgatggcgat taccgccaac gaactggata cggcacgtcg
     1021 ccgtacaacg ggttctatgg atgcgctgaa agcggccgga ttcccggaaa aacaaattta
     1081 tcaggtacct accaaatcta acgacatccc gggggcattt gacgctgcca actcaatgct
     1141 ggttcaacat ccggaagtta aacattggct gatcgtcggt atgaacgaca gcaccgtgct
     1201 gggcggcgta cgcgcgacgg aaggtcaggg ctttaaagct gccgatatca tcggcattgg
     1261 cattaacggt gtggatgcgt tgagcgaact gtctaaagca caggcaaccg gcttctacgg
     1321 ttccctgctg ccaagcccgg acgtacatgg ctataaatcc agcgaaatgc tttacaactg
     1381 ggtagcaaaa gacgttgaac cgccaaaatt taccgaagtt accgacgtgg tgctgatcac
     1441 gcgtgacaac tttaaagaag aactggagaa aaaaggttta ggcggtaagt aatttgccgg
     1501 aaaaattccc ctctgcatga tgcagagggg gtgtgaacga ccagtgattc acggagacgt
     1561 tatgcaacag tctaccccgt atctctcatt tcgcggcatc ggtaaaacgt ttcccggcgt
     1621 taaggcgctg acggatatta gttttgactg ctatgccggt caggttcatg cgttgatggg
     1681 tgaaaatggc gcaggaaaat caactctctt aaaaatcctc agcggcaact atgcgccaac
     1741 cacgggttct gtagtgatta atgggcagga aatgtccttt tccgacacga ccgcagcact
     1801 taacgcgggt gtggcgatta tttaccagga actgcatctc gtgccggaaa tgaccgtcgc
     1861 ggaaaacatc tatctcggcc agctgccgca taaaggcggc attgtgaatc gctcattgct
     1921 gaattatgag gcgggtttac aacttaaaca tcttggtatg gatattgacc cggacacgcc
     1981 gctgaaatat ctctccattg gtcagtggca gatggttgaa atcgccaagg cgctggcgcg
     2041 taacgctaaa attatcgcct ttgatgagcc aaccagctcc ctttctgccc gcgaaatcga
     2101 caatcttttc cgcgttattc gtgaactgcg aaaagagggg cgggtgatct tatacgtttc
     2161 tcaccgtatg gaagaaatat ttgccctcag cgatgccatc actgtcttta aagatggacg
     2221 ttatgtcaaa acctttaccg atatgcagca ggttgaccac gacgcgctgg tgcaggcgat
     2281 ggtcgggcgc gacattggcg atatctacgg ctggcaacct cgtagttatg gcgaggagcg
     2341 cctgcgtctt gatgctgtga aagcaccagg cgtgcgtacg ccaataagtc tggcggttcg
     2401 cagtggtgaa attgttgggc tgtttggtct ggtaggggcg gggcgtagcg aattaatgaa
     2461 aggcttgttt ggcgggacgc aaatcaccgc cggtcaggtt tatatcgacc aacagccgat
     2521 cgatattcgt aaaccgagcc acgccattgc cgcaggcatg atgctctgcc cggaagatcg
     2581 caaagcggaa ggcattattc ccgtgcactc cgttcgcgac aatatcaaca tcagtgccag
     2641 acgcaaacat gtgctcggcg gttgtgtaat caacaacggt tgggaagaaa acaatgccga
     2701 tcagcacatt cgttcgctca acatcaaaac gccgggcgct gagcaactga tcatgaatct
     2761 ctcaggcgga aatcagcaaa aagccattct gggccgctgg ttatcggaag agatgaaggt
     2821 cattttgctg gatgaaccta cgcgcagcat tgatgttggt gctaagcacg aaatatataa
     2881 cgtaatttat gcgctggcgg cgcagggtgt ggcggtgctg tttgcctcca gcgacttacc
     2941 tgaagtcctc ggcgttgccg accggattgt ggtgatgcgg gaaggtgaaa tcgccggtga
     3001 attgttacac gagcaggcag atgagcgtca ggcactgagc cttgcgatgc ctaaagtcag
     3061 ccaggcagtt gcctgagtaa ggagagtatg atgtcttctg tttctacatc gggttctggc
     3121 gcacctaagt cgtcattcag cttcgggcgt atttgggatc agtacggcat gctggtggtg
     3181 tttgcggtgc tctttatcgc ctgtgccatt tttgtcccaa attttgccac cttcattaat
     3241 atgaaagggt tgggcctggc aatttccatg tcggggatgg tggcgtgtgg catgttgttc
     3301 tgccttgctt ccggtgactt tgacctttct gtcgcctccg taattgcctg tgcgggtgtc
     3361 accacggcgg tggttatcaa cctgactgaa agcctgtgga ttggcgtggc agcggggttg
     3421 ctgcttggca ttctctgtgg cctggtcaat ggctttgtta tcgccaaact gaaaataaat
     3481 gctctgatca caacactggc aacgatgcag attgttcgag gtctggcgta catcatttca
     3541 gacggtaaag cggtcggtat cgaagatgaa agcttctttg cccttggtta cgctaactgg
     3601 ttcggtctgc ctgcgccaat ctggctcacc gtcgcgtgtc tgattatctt tggtttgttg
     3661 ctgaataaaa ccacctttgg tcgtaacacc ctggcgattg gcgggaacga agaggctgcg
     3721 cgtctggcgg gtgtaccggt tgttcgcacc aaaattatta tctttgttct ctctggcctg
     3781 gtatctgcga tagccggaat tattctggct tcacgtatga ctagtgggca gccaatgacg
     3841 tcgattggtt atgagcttat tgttatctcc gcctgcgttt taggtggcgt ttctctgaaa
     3901 ggtggcatcg gaaaaatctc atatgtggtg gcgggtatct taattttagg caccgtggaa
     3961 aacgccatga acctgcttaa tatttctcct ttcgcgcagt acgtggttcg cggcttaatc
     4021 ctgctggcag cggtgatctt cgaccgttac aagcaaaaag cgaaacgcac tgtctgatgc
     4081 ttttttccac aacaatttaa cgttttttcc caccacagcc agccgccaca acggttggct
     4141 gttcttcatt gcaaatggcg acccccgtca cactgtctat acttacatgt ctgtaaagcg
     4201 cgttctgcgc aaaacaataa gaaaagagaa ggaggagagc cgggtgacag aaccgttaac
     4261 cgaaacccct gaactatccg cgaaatatgc ctggtttttt gatcttgatg gaacgctggc
     4321 ggaaatcaaa ccgcatcccg atcaggtcgt cgtgcctgac aatattctgc aaggactaca
     4381 gctactggca accgcaagtg atggtgcatt ggcattgata tcagggcgct caatggtgga
     4441 gcttgacgca ctggcaaaac cttatcgctt cccgttagcg ggcgtgcatg gggcggagcg
     4501 ccgtgacatc aatggtaaaa cacatatcgt tcatctgccg gatgcgattg cgcgtgatat
     4561 tagcgtgcaa ctgcatacag tcatcgctca gtatcccggc gcggagctgg aggcgaaagg
     4621 gatggctttt gcgctgcatt atcgtcaggc tccgcagcat gaagacgcat taatgacatt
     4681 agcgcaacgt attactcaga tctggccaca aatggcgtta cagcagggaa agtgtgttgt
     4741 cgagatcaaa ccgagaggta ccagtaaagg tgaggcaatt gcagctttta tgcaggaagc
     4801 tccctttatc gggcgaacgc ccgtatttct gggcgatgat ttaaccgatg aatctggctt
     4861 cgcagtcgtt aaccgactgg gcggaatgtc agtaaaaatt ggcacaggtg caactcaggc
     4921 atcatggcga ctggcgggtg tgccggatgt ctggagctgg cttgaaatga taaccaccgc
     4981 attacaacaa aaaagagaaa ataacaggag tgatgactat gagtcgttta gtcgtagtat
     5041 ctaaccggat tgcaccacca gacgagcacg ccgccagtgc cggtggcctt gccgttggca
     5101 tactgggggc actgaaagcc gcaggcggac tgtggtttgg ctggagtggt gaaacaggga
     5161 atgaggatca gccgctaaaa aaggtgaaaa aaggtaacat tacgtgggcc tcttttaacc
     5221 tcagcgaaca ggaccttgac gaatactaca accaattctc caatgccgtt ctctggcccg
     5281 cttttcatta tcggctcgat ctggtgcaat ttcagcgtcc tgcctgggac ggctatctac
     5341 gcgtaaatgc gttgctggca gataaattac tgccgctgtt gcaagacgat gacattatct
     5401 ggatccacga ttatcacctg ttgccatttg cgcatgaatt acgcaaacgg ggagtgaata
     5461 atcgcattgg tttctttctg catattcctt tcccgacacc ggaaatcttc aacgcgctgc
     5521 cgacatatga caccttgctt gaacagcttt gtgattatga tttgctgggt ttccagacag
     5581 aaaacgatcg tctggcgttc ctggattgtc tttctaacct gacccgcgtc acgacacgta
     5641 gcgcaaaaag ccatacagcc tggggcaaag catttcgaac agaagtctac ccgatcggca
     5701 ttgaaccgaa agaaatagcc aaacaggctg ccgggccact gccgccaaaa ctggcgcaac
     5761 ttaaagcgga actgaaaaac gtacaaaata tcttttctgt cgaacggctg gattattcca
     5821 aaggtttgcc agagcgtttt ctcgcctatg aagcgttgct ggaaaaatat ccgcagcatc
     5881 atggtaaaat tcgttatacc cagattgcac caacgtcgcg tggtgatgtg caagcctatc
     5941 aggatattcg tcatcagctc gaaaatgaag ctggacgaat taatggtaaa tacgggcaat
     6001 taggctggac gccgctttat tatttgaatc agcattttga ccgtaaatta ctgatgaaaa
     6061 tattccgcta ctctgacgtg ggcttagtga cgccactgcg tgacgggatg aacctggtag
     6121 caaaagagta tgttgctgct caggacccag ccaacccggg cgttcttgtt ctttcgcaat
     6181 ttgcgggagc ggcaaacgag ttaacgtcgg cgttaattgt taacccctac gatcgtgacg
     6241 aagttgcagc tgcgctggat cgtgcattga ctatgtcgct ggcggaacgt atttcccgtc
     6301 atgcagaaat gctggacgtt atcgtgaaaa acgatattaa ccactggcag gagtgcttca
     6361 ttagcgacct aaagcagata gttccgcgaa gcgcggaaag ccagcagcgc gataaagttg
     6421 ctacctttcc aaagcttgcg taggagcggt taatctcccg taagtggaac taacagcaca
     6481 tcgacctcac ttgaggcaat cactcttttc gccgagcagg atgctcgcga aaagaaactg
     6541 tgattgtgat taccgcaaat caccaaatcg aaatgatgtt tgtgacatac ttccagaata
     6601 tgttcgctta actcaccgta ggcaataaac gtcttgtcaa cgggataccc cgcatcctga
     6661 attaacttat caagaaagct ttgcgtttct tcttgcatca cgctgcgtaa atcttcaagc
     6721 atcggcgcgg ctaattgatt gtacatttcc gggtcggaag cgagagtaat taaactgatg
     6781 tgtcccttta ctggcctggc gatagatacc gcttttgcca gcagttgctg actttccggt
     6841 gtaaccgcaa cagcgacaag aatattgcta tagctcataa cctgttcctt attctgtgaa
     6901 cttcaggtga cattaaagct ttactcctaa tggagaaacg acgcaatccc aactcggtca
     6961 aacaggtaaa tattagtctg attattagta aagacaat
//
LOCUS       DAZPLI010000189         6972 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZPLI010000189 DAZPLI010000000
VERSION     DAZPLI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6972
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.190"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            361..1131
                     /locus_tag="ACHUU3_003783"
     CDS             361..1131
                     /locus_tag="ACHUU3_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR2083746.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1122..1595
                     /locus_tag="ACHUU3_003784"
     CDS             1122..1595
                     /locus_tag="ACHUU3_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR2083747.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1703..2242
                     /gene="dnaT"
                     /locus_tag="ACHUU3_003785"
     CDS             1703..2242
                     /gene="dnaT"
                     /locus_tag="ACHUU3_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR2083748.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2245..2982
                     /gene="dnaC"
                     /locus_tag="ACHUU3_003786"
     CDS             2245..2982
                     /gene="dnaC"
                     /locus_tag="ACHUU3_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR2083749.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3031..3525
                     /gene="yjjA"
                     /locus_tag="ACHUU3_003787"
     CDS             3031..3525
                     /gene="yjjA"
                     /locus_tag="ACHUU3_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR2083750.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3779..6070
                     /gene="opgB"
                     /locus_tag="ACHUU3_003788"
     CDS             3779..6070
                     /gene="opgB"
                     /locus_tag="ACHUU3_003788"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR2083751.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6209..>6972)
                     /gene="lgoD"
                     /locus_tag="ACHUU3_003789"
     CDS             complement(6209..>6972)
                     /gene="lgoD"
                     /locus_tag="ACHUU3_003789"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFR2083752.1"
                     /translation="PYVACQQCPACKSGRTNCCEKISVIGVHQDGGFSEYLSVPVANI
                     LPADGIDPQAAALIEPFAISAHAVRRAAIAPGEQVLVVGAGPIGLGAAAIAKADGAQV
                     VVADTSPARREHVATRLELPLLDPSAEDFDAQLRAQFGGSLAQKVIDATGNQHAMNNT
                     VNLIRHGGTVVFVGLFKGELQFSDPEFHKKETTMMGSRNATPEDFAKVGRLMAEGKIT
                     ADMMLTHRYPFATLAETYERDVINNRELIKGVITF"
BASE COUNT         1734 a         1828 c         1748 g         1662 t
ORIGIN      
        1 cacattcatt taatttataa ttaaatgata tcccttcccg cagaatgaaa aatcacacaa
       61 ccaatattta ttcaatgaaa tctgatggtt ttaacttgtt aacattaaaa aacgtgcccg
      121 gttatctatt atcctgattc gtaaaggcca aacaataaat tcattatagt gataatattc
      181 atgtgatagt ttgtttgcgg cgagagataa ttcgcttttt atcaccgagc ttgtcccgat
      241 aaccaggatt aatcagcgca attttgatct taacctaccg caaaacaacc gtgcttcatg
      301 atacaaatgg attctcatta tctgaacaat acgcagcacg tctatgacaa agggcgagtt
      361 atgcaaactg agcaacagcg agccgtaaca cggctttgta tccagtgtgg attatttctt
      421 ttgcaacatg gtgcggaaag cgcgttggtt gatgagcttt cctcacgact gggtcgggcg
      481 ttggggatgg acagcgtcga aagttctatc tcttcgaacg ccatagtgct gacaaccatt
      541 aaagatgggc aatgcctgac atcgacacgt aaaaatcacg atcgcggcat taatatgcat
      601 gtggtgactg aagtccagca cattgtgatt ctggcggagc accatctgct ggattacaaa
      661 ggcgtagaga aacgatttag ccaaattcag ccattacgtt acccaagatg gctggtagcc
      721 ttaatggttg gcctttcttg cgcctgtttc tgcaaactca ataaaggtgg ctgggatggt
      781 gccgtcatca ccttctttgc cagtacggcc gcgatgtata tccgccagct gctggcacaa
      841 cgtcatcttc atccgcagat caacttttgc ctcaccgctt tcgccgccac caccatttcc
      901 ggattgcttt tgcaacttcc cactttcagc aatacaccca ctatcgcgat ggccgccagc
      961 gttctgctgc tggtgccggg atttccgttg attaatgccg tcgccgatat gtttaaaggc
     1021 cacatcaata ccggactggc acgctgggcg atcgccagtc tgctgacact ggctacctgc
     1081 gtcggcgtgg tgatggcact gacgatttgg gggctacgcg gatgggtgtg atcgagtttc
     1141 tgtttgcatt ggcgcaggat atgatcctcg ccgccattcc agcggtcggc tttgcgatgg
     1201 tgttcaacgt tcccgttcgg gcattacgct ggtgtgcgct gcttggcgcg attggtcatg
     1261 gttcacgaat gatcttaatg accagcgggc tgaatattga gtggtcaacc tttatggcct
     1321 ccatgctggt cggcaccatt ggtattcaat ggtcgcgctg gtatctggcg catccgaaag
     1381 tgtttaccgt ggcggccgtt atccctatgt tcccgggcat atcggcttat accgcaatga
     1441 tttcggcggt aaaaatcagc cagttaggtt acagcgaacc gttgatgatt accctgttaa
     1501 ccaactttct tacagcttca tcgattgttg gtgcgttatc catcggtctt tccattcctg
     1561 gattatggtt gtaccgcaag cgccctcgcg tataaaattg cctcgcttaa agcacacgga
     1621 tgagagacag cctcctctcc tccgtgtgtt actataaaag ttatctccct tctcgttcat
     1681 cgttccatat ttgagaaaca gtatgtcttc cagagttttg accccggacg tcgttggtat
     1741 tgacgccctg gtacacgatc accaaaccgt tctggcaaaa gctgaaggcg gtgtggttgc
     1801 cgtatttgct aacaatgccc cggcgtttta tgccgtcacg cctgcacgcc tggctgaact
     1861 gctggcgctg gaagaaaagc tggcgcgtcc gggaagcgat gtcgctctgg acgatcaact
     1921 ctatcaggaa ccgcaagccg ctcccgttgc tgtacccatg gggaaattcg ccatgtatcc
     1981 ggactggcaa cccgatgccg attttatccg cctggcggcg ctatggggtg tggcactaag
     2041 agagccggtg accaccgaag aactggcctc attcattgcc tactggcagg cggaaggtaa
     2101 agtctttcac catgtgcagt ggcaacaaaa actggcgcgc agcctgcaaa tcggtcgtgc
     2161 cagcaacggc ggactgccga aacgagatgt gaatacggtc agcgaacctg acagtcaaat
     2221 tccaccagga ttcagagggt aacgatgaaa aacgttggcg acctgatgca acgcctgcaa
     2281 aaaatgatgc ctgcccatat caaacctgca ttcaaaacgg gtgaagaact actggcctgg
     2341 cagaaagaac aaggagcgat ccgctccgcc gctctcgaac gtgaaaatcg ggcgatgaaa
     2401 atgcagcgca cctttaaccg ctccggtatt cggccactgc atcagaactg ctcctttgag
     2461 aactatcgcg ttgagtgtga agggcagatg aatgcgttaa gcaaagcgcg ccagtacgtc
     2521 gaagagtttg acggcaacat cgccagcttc atcttttctg gtaagccagg aaccggcaaa
     2581 aaccatctgg cggcggcaat ctgcaacgag ctgctgctac gcggtaaatc cgtattgatc
     2641 atcaccgtgg ccgatattat gtcggcgatg aaagatacct tcaggaatag cggtaccagt
     2701 gaagaacaac tgcttaacga tctgagcaac gttgatctgc tggtgatcga tgagatcggc
     2761 gtgcagaccg aatcgaaata cgaaaaagtg atcatcaacc agatcgtcga tcgccgctct
     2821 tcttccaaac gcccaaccgg gatgctgacc aacagtaata tggaagagat gaccaagttg
     2881 ctgggcgaac gcgtgatgga ccgtatgcgc ctgggtaaca gtttgtgggt gatcttcaac
     2941 tgggatagct accgtagccg ggtaacaggt aaagagtatt aatccccgtt tctggcggta
     3001 tactgcgaca caattccacc ttgagtaatg atgatgaaaa ctgttaaaca tcttctgtgc
     3061 tgtgccattg ccgccagcgc attaatttcc accggggtgc atgctgcgtc ctggaaagat
     3121 gcgctctcca gcgccgccag cgaacttggc aaccaaaaca gcacgacaca ggaaggcggt
     3181 tggtcgctcg cgtcattaac taacttgctt agcagcggga accaggcctt aagcgcagat
     3241 aacatgaaca acgccgcagg cattctgcaa tactgcgcga agcaaaagct ggcgtcggta
     3301 accgatgccg aaaacatcaa gaaccaggtg ctggaaaagc tgggcctgaa cagtgaagag
     3361 caaaaagaag acaccaacta tctggatggc attcagggtt tgctgaaaac caaagatggt
     3421 cagcaactca atctggataa catcggaacg actccgctgg cagaaaaggt gaaaaccaaa
     3481 gcctgcgatc tggtgttaaa gcaggggctg aacttcattt cctgaaccgc gaaacgccgc
     3541 gttttagctg ttacccggct gacgcggctc tctcctgccc ttttccctgc tcctggacgg
     3601 ttttccccct gttacacggg aagcgaccgc gatcaaaaaa gtctgtttat aaactaattc
     3661 ctaacaacat cgcatttttt tgacactaga attgcagcaa tatggcgacg ccattacatt
     3721 gtgcttctca aaaagcttaa acgttggcta aacggcccgg tttagtgagg atcgcccgtt
     3781 gtcagaacta ctctctttcg ccctttttct cgcctctgtg ctgatttacg catggaaagc
     3841 gggacgtaac acctggtggt ttgcagccac attaacggtg ctggggctat ttgtcgtttt
     3901 aaatatcacc ctgtttgcca gcgactattt tactggcgat ggtattaacg acgcggttct
     3961 ctatacctta accaacagcc tgaccggtgc tggcgtcagc aaatacatcc tgccgggtat
     4021 cggcattgtg ctggggctga cagcggtgtt cggtgcgctg ggctggatcc tgcgccgtcg
     4081 tcgccatcat ccgcaccatt ttggttacag cctgctggcg ctcttactgg cgctgggttc
     4141 ggtggacgcc agcccggcat ttcgtcagat aacggaactg gtgaaatccc agtcacgcga
     4201 tggcgacccg gactttgcgg cttattataa agagccgtcg aaaactatcc ctgacccgaa
     4261 actcaacctg gtttatatct acggcgaaag tctcgagcgg acctattttg ataacgaagc
     4321 tttcccggat ctcacgcctg aactgggcgc gttgaaaaat gaaggcctgg atttcagcca
     4381 cacgcagcag ctgccaggaa cggattacac gattgcgggc atggtggctt ctcagtgcgg
     4441 cataccgctg tttgccccct ttgaaggcaa cgcctccgcc tctgtctcca gcttcttccc
     4501 gcagaacatc tgtctgggcg atatcctgaa aaactcgggt tatcagaact atttcgtgca
     4561 gggcgcgaat ctgcgttttg ccggtaaaga tgtgttcctg aagtcgcatg gcttcgacca
     4621 cttatacggc tcagaagagc tgaaaagcgt ggtggccgac ccgcactatc gcaacgactg
     4681 gggattctac gacgataccg ttctcgatga agcgtggaaa aagtttgaag agctttcccg
     4741 ctcaggtcag cgattctcac tgtttaccct gacagtcgat acccatcacc cggatggttt
     4801 tatctcgcgt acctgtaacc gcaaaaaata tgattttgac ggtaagccga accagtcatt
     4861 cagcgcggta agttgcagcc aggagaatat cgcggcgttt atcaacaaaa tcaaagcgtc
     4921 accgtggttt aaagataccg tcatcgtcgt ctcttctgac catttagcga tgaacaacac
     4981 ggcgtggaaa tacctcaata agcaggatcg caataacctg ttttttgtca ttcgtggcga
     5041 caagccgcag caagagacgc tggcagtgaa gcgtaacacg atggataacg gcgcgacggt
     5101 gctggacatt ctcggtggcg ataactatct cggacttggt cgtagcagtt tatccgggca
     5161 gtcgatgtcg gaaatcttcc tcaatatcaa agagaaaaca ttggcgtgga agccggatat
     5221 catccgcctg tggaaattcc ctaaagagat gaaagagttc accatcgacc agcagaaaaa
     5281 catgattgcc ttctcgggta gccatttccg tttgccgctg ctgttgcggg tttcagacaa
     5341 acgcgtggaa ccgctgccgg aaagcgaata ctcagcaccg ctgcgtttcc agctggccga
     5401 tttcgctcca cgcgacaatt tcgtctgggt tgaccgttgc tataagatgg cacaactctg
     5461 ggctccggaa ctggcactct ccaccgactg gtgtgtctcg caagggcagc ttggcggtca
     5521 gcaaattgtt cagcatgttg acaaaacaac atggcagggc aaaacggcat ttaaagatac
     5581 ggtcatcgac atggcgcgtt ataaaggcaa tgtcgatacg ctgaaaattg ttgataacga
     5641 tattcgctac aaagccgaca gtttcatctt caacgtcgcc ggtgcgccag aagaggtgaa
     5701 acagtttagt gggatttccc gtccagagtc gtggggccgc tggtccaacg cgcagctggg
     5761 cgatgaagta aaaatcgagt acaagcatcc gctgccaaag aaatttgacc tggtgattac
     5821 cgccaaagca tacggcaata acgccagccg tcctattccg gtacgcgtag gcaatgaaga
     5881 acaaaccctt gtgctgggca atgaagtgac caccaccaca ctgcatttcg ataacccaac
     5941 cgatgccgac acactggtaa ttgtgccgcc ggaacctgtc tcaaccaacg aagggaatat
     6001 cctcggacac tcgccgcgta agctcgggat cggcatggtg gaaattaaag tggtggaacg
     6061 tgaaggataa ttcctgactg atgtgtaggt cgcataaggt gcgacctaca caccttctgc
     6121 ggcctacaaa acatcattga gaaacagaaa aaaacagcgc ccaacacttc cccggacgct
     6181 gcatggcaac tttaataatc tcgccttatc agaaagtaat tacaccctta atcaactcac
     6241 gattgttaat cacatcgcgc tcgtaggttt ctgccagcgt ggcgaacgga tagcgatggg
     6301 ttaacatcat gtcagcagtg atttttcctt ccgccatcag tcgaccgact ttagcaaaat
     6361 cttccggcgt ggcgttgcgg ctacccatca tcgtcgtttc ttttttatgg aattccggat
     6421 cggagaactg caactcacct ttaaacaggc cgacaaatac caccgtgccg ccgtgacgaa
     6481 tcaaattcac ggtgttattc atcgcatgtt gattacctgt cgcgtcgatc actttctgcg
     6541 ccagcgaacc accaaactgc gcccgtagct gcgcgtcaaa atcttcggct gacgggtcca
     6601 gtaaaggtaa ttccagacgc gttgccacat gttcacggcg cgccggactg gtatccgcca
     6661 ccaccacctg tgcgccatcg gctttagcga ttgccgccgc gcccagaccg attggccccg
     6721 ccccgaccac cagcacctgc tcgccgggag caatggctgc gcgacgcacc gcatgagcgc
     6781 taatagcgaa aggttcaatc aatgctgccg cctgcgggtc aataccgtct gcgggcaaaa
     6841 tgttcgccac cggcacgctc aaatactcac taaaaccgcc atcctgatgc acgccaatga
     6901 ctgaaatttt ttcacagcaa ttggtacgcc cgcttttaca cgccgggcat tgctgacagg
     6961 caacataagg ga
//
LOCUS       DAZPLI010000190         6933 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZPLI010000190 DAZPLI010000000
VERSION     DAZPLI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.191"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..214)
                     /gene="cas6e"
                     /locus_tag="ACHUU3_003790"
     CDS             complement(<1..214)
                     /gene="cas6e"
                     /locus_tag="ACHUU3_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR2083753.1"
                     /translation="MAVWGTHRQSGAVAQQGGTMYLSRITLHTGQLSPAQLLHLVDRG
                     EYVMHQWLWDLFPGGKERQFLYRREEL"
     gene            complement(139..885)
                     /gene="cas5e"
                     /locus_tag="ACHUU3_003791"
     CDS             complement(139..885)
                     /gene="cas5e"
                     /locus_tag="ACHUU3_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFR2083754.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(896..1951)
                     /gene="cas7e"
                     /locus_tag="ACHUU3_003792"
     CDS             complement(896..1951)
                     /gene="cas7e"
                     /locus_tag="ACHUU3_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFR2083755.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRVFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(1963..2499)
                     /gene="casB"
                     /locus_tag="ACHUU3_003793"
                     /gene_synonym="cse2"
     CDS             complement(1963..2499)
                     /gene="casB"
                     /locus_tag="ACHUU3_003793"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFR2083756.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(2496..4058)
                     /gene="casA"
                     /locus_tag="ACHUU3_003794"
                     /gene_synonym="cse1"
     CDS             complement(2496..4058)
                     /gene="casA"
                     /locus_tag="ACHUU3_003794"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFR2083757.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDISAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFI
                     QDFEALTGDKVPVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWINVMPQDEADLPLPKKFDD
                     LVFPWLGPTRISELAGAVVTDEQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVLLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVDLERIMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(4156..6855)
                     /locus_tag="ACHUU3_003795"
     CDS             complement(4156..6855)
                     /locus_tag="ACHUU3_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFR2083758.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGL
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKE
                     DSSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT         1696 a         1871 c         1627 g         1739 t
ORIGIN      
        1 ggagttcttc ccgacgataa agaaattgcc tttctttgcc gccagggaaa agatcccaca
       61 gccactgatg catcacatat tcaccgcgat ccaccagatg cagcaactgc gcaggcgaaa
      121 gctggccggt atgtaaggtt attcttgaga ggtacatggt tcctccttgc tgagccacgg
      181 cccctgattg acggtgcgtt ccccaaacag ccactgctga cgatttaaag gaacatcccg
      241 gcggcgtaat attttgctcg cgaccaggcc gtcgtgtttc ccttcccacc agcattcatc
      301 ctgaagtttc gggagtgaga ctttcagttt atggaaatga tcctgatact gttggtatgc
      361 attacataag gcatcacacg cgttgccttc gagcagtaac ggcgcaagag gtaatgccag
      421 cggatgactt tttcgcccca gataaagcgg aaaaactgga tggcgtaaac cgtcctgcaa
      481 ctgttcgagg ctgtaaggcg catcggctgt tgttgccacc gccaccatcc accaggcatc
      541 ggtgtagtag tcgcgccggg agataatcgc gctcaaaagc tcagggtcgc tcaactcttc
      601 acgacggctg aaataacgtg ctttacgcac ctcttttggc atctggaccg tgtgataatc
      661 ccgtgcccag cgcgggttac ggctggcgca aaccaccagt gaatagtggc gattaaacgc
      721 gtttaagcgt tcggtatcat cacgccgaat ccctaccccg gcagccagta gccccagcaa
      781 tgctgagcgc gaaggaagtt catgggtatg acgaacttca cctggagcat cgacacccca
      841 ggacgccatc ggcccatgaa gctgaaaaat cagatattgg ctcataagcc gccccttacg
      901 cgcagataaa gtccagcacg tccttcatgc ttccctgttt gttcatcacg tcaaaacttg
      961 cgcattcggt cttctgttcg tagaccgtat tcatgttttc gcgaagcgtt gtaatacgct
     1021 gcaccgccac atctaactga cgtgtgccat taatgggttc atagaaagcc gccgccagag
     1081 agcgtggttg atcggtgcct ttttctgcca gcgcccagga agcataggca cggctggcaa
     1141 agctattttg tttaccggtt ggggaaactt taagcgcggc ttccgtaaaa acgcgtatgg
     1201 tctgattagc taacgcttcg tcaccgccga ggttttctac cagcagatct ttatcaatgc
     1261 agatataggt gtagaaaagt gctgaaccga atccggtttc tccgagatgc ccggcaccgg
     1321 catcttcaga ggcctggcgc aaatcatcaa cggcggtgaa aaaatcatct tcgacaatcg
     1381 tttcactgac accaaacgca tgcgcgacct ggcaggcggc ttcaacatta aactcaggtt
     1441 tattcgccag catacgacca aacatagcga tatctaccgc tatacggtct ttacgtaaca
     1501 acgcgagatc ttcctctttt ggcgcgcgct tttcttctgc cagttgatga gcaagcgctt
     1561 ttacggcgtc aaattctgcc gggctgatat gaactaattg ttcagtttcg gcgttagtga
     1621 gcggatcttt tggttttttg tcatttttag ctttcccaag ataatcagca atttttgccg
     1681 cccattcgat ggctttttta tcttcaatcc ctttctcaat caggatagtt gccgcctcac
     1741 gcgcaatacg cccactgcga ataccaatat ggcccgccag tgcctgttca aaaagtgcag
     1801 aagtgcgcca cgcacgtttc agactttgcg aggaaacgcg cagacgcgtt gctccaccca
     1861 gcaccacagt ttttggcgca ccggtgtcat cacggttaag gttggcagca gggtaagcgg
     1921 ttaacacatg aagctgaata aatgttgtca tgagatagtc ctttattgat tttgttcgtt
     1981 atcggcgtcg ccagcctggt aatattccag cgcccagcgg atacggataa attcggttgg
     2041 ccgctgctgg cggcggtgat gattgagcag gtcgtcgctc tcctggcacc agcggaaaat
     2101 gccatctgcc agggagtcaa gattgacgga accgtttaat aatctgacgg cgcggcgtaa
     2161 ctggcgcagt aattcatccg gtgtttttac tgctgacagg cgagtaaaac gcccctttga
     2221 cattaccgcc gccagttgcg cggcaaaagg ctgccgttca tcaatggctt taacattggc
     2281 gctaactgcg gctatcagcg ccagtgcggt gatacgccac tcaggttcat cctgccactt
     2341 tatttgtctg ttcttcagaa acaggcgaaa tccatccgtc agacaaacat cattaaccgt
     2401 cgtactacgc cgcagactgg cacgttcgcc gcgtttctcc tgcaattcct cgtgccattt
     2461 gcgcagcgtg gctttgtgct cctcttttac aatactcatt cagcagcctc ctgctttttc
     2521 tccctggcgg ctttagcact ttgcttctcc gccgatgttg taaaatattt cttgcgcgcg
     2581 gtcatgatgc gttccaaatc aacgggctca taaggattgg tgaatacacg ctcgtcaaaa
     2641 tcctgacgcg cgaataacca aatttcctta ttccatttgc tgagtaattc atccgcatcc
     2701 tgaccttctt caatttggcg cactaacctc aggaagcgat gctgagtttt gttccagaag
     2761 tcgatatcca caaaactaaa atcacccctt gcaccttttg gatcggagaa ccatgcttct
     2821 ttcaatgcac tccgtaacag acttagaatc cgtgaagccg tttgcgcagc cagccgcagt
     2881 ttcggtatct ggccttcttt tttattgagc agcagcggga aatgatgttc gtaccaacag
     2941 cgcgctttca tgttgtcgaa atcatagcca aatccccaca ggcccacttt tgcctgcttg
     3001 agactgctgg cattaaagag tttcaccacc agcgcgggaa gttccgtatt gttttctgac
     3061 ttacccgttt cgataaggcc taaccagtcg cgccagatta aaccgcccgg ttgtggtttg
     3121 accgagtaaa actcgccgcc ctctttaagt agtacacggt aaggcgttaa gggatgctgc
     3181 cacatggcat agttagcgcc gtaatttttg gtcgtcatca ggctcagaag cgcgtcgctc
     3241 tgctcaccgc aaatatcgca gttgccgact gtggtggtat taaaatcaat acgaatacgg
     3301 cgaggcattc cccagtacgc ctggagttta ttgacctgct catcggttac caccgcaccg
     3361 gccagttcgc tgatgcgcgt cgggccaagc caggggaaaa ccagatcgtc aaattttttg
     3421 ggtagcggta aatcggcttc atcctgcggc atgacattga tccacagttt gcgccacaag
     3481 ggagtttgtt gattaccctg atattcctgt aattcaatca gagtggtcat cggcccaccg
     3541 ccgcgtaaac cggtgcggta gcctttgcca cctgacggcg cattcaactg tagggagaac
     3601 agagctaacg cagagcaatg tgggcacagg tattccgtta caccacgctt gataaagtgg
     3661 tctttattaa acttcgttgt ttgagcgccg ggaatctcag gcagtagcga agcgaccgga
     3721 actttatctc cggtgagcgc ctcgaaatcc tgtataaatg aaggggaatc tggcccaaac
     3781 tggaaagcat gttctaatga cagcaatgct tcccgtagct tttcagcttc cagtccgtct
     3841 tcccagatat catcccaacg acgatgatct tttggcgcga agctggtttg tagtagcccc
     3901 agcaaaaact gccataccgc accctggaga tctgcccgcg gcgcagaaat atcgacaaca
     3961 ttttcatccg ccagatcgac tggcgccagt ttgcctgtcg ttccgtcttt aaaacgaacg
     4021 ggcaaccacg gggttgtcag aagtgaaaac gagttcatac gcatcctctc gtaaaaaaga
     4081 ccatcccagg tcggttgtcc atctttccat ccttttcggt gtatcaacta ttcagagagt
     4141 cctgtcgcag acatctcaac caaccttgcc aatcaatcct tccgtagccg aatacccgca
     4201 actttcgtca tcgttgacta aaataacgtt tgccatctcc ggatcttgtc gctgttcaat
     4261 gcaccacagc ctgaacgcct ctccctccag taaagaaaac tcatcccgat gttttttcca
     4321 ccagcttcgg cgcactctga caacgctcat ttcccatgcg tgagcaccgg tggcataagg
     4381 cttcaccaca ccgtcaatac aggtagccag ccacagggaa acagactcct cagccagacg
     4441 tgtcgacagt ttttctggga ggtaatcgtt gatattggcg gcatagccgg gcctgaagtt
     4501 cagcacaaac tttttagcca ttgcgcgatc acagtaatat ttgcccactt gctcctgctc
     4561 gctgcgggca aatccttccg gcattaccac gtcctcaccg tagacggatt caataagaag
     4621 ccgggctgcg tgtggcatct gaatagcacc ttgctcacgc agcacacgct gcgtcagcca
     4681 gattcgccca tgatcgggat agacaaatgc actgttacgc atggcactgc caaaccattc
     4741 gtcgccagga gagtcgtccc agacgggagc cagaatcagc aattcagggg gggaacgctc
     4801 gtcctttccg tcacgcttta actgaccatt aatatcgcgg atatgccgct gtaatcgtcc
     4861 tgctcgctga atcagcaaat caacaggggc caggtcggag atcatttcgt ccaggtcaca
     4921 atcgacactc tgctctaata cctgagtaca aataaggact tttccggcac gctgtgagct
     4981 atcttcttta ccaaagcgtg ccagtgtctc cgtttcaatt cgctggcgat cgctaaaagc
     5041 aaagcggcta tgaaaaagtg aaaggctgga agcaggaatg acgccgcggg caagcagctg
     5101 acgatgaacc ttaatagcgt catcgacaga attccggatc caggcgatgc atttcccctg
     5161 acttaccgcc gattcgatac gcgcaataca ctcttgttca ctatgaagcc aacccacgct
     5221 gacgctacgc tcaacgtctt tgcgcgtcgc tacccggtgt gagttcacat cggatttcgt
     5281 gacatgcgtc agccaggggt aatcatcctt ttccaggaac ggagcttctt gcaggccctc
     5341 tgtaccccgc gcaaaggcag cgacgagttt gtcgcgctgc tgttgagata acgtggcaga
     5401 aagcaaaata acgctgtttc cgccacgcgc ctgccgctcg atcaaccctt caagaatgca
     5461 cgacatgtaa gcgtcacagg cgtggatctc atcagccagc aggattttgt tactcagccc
     5521 cagaagccgc agattattat gtttgaacgg catcaccgcc atcatcgcct gatccagcgt
     5581 gccgacgcca atttcagcca gcaacgcctt cttgttactg tcggcaaacc aggccgcaca
     5641 tccctgactg aacgtttgtt catccggttc ttctgacccg actaaatcac cagaccagag
     5701 tgattcatta aagcggtcca ttaatgtgcg ggcactatgt gccagcacca ggctggggcg
     5761 ggattctggc gaatagaaag caagccaggt tttgaccagc cgatcgtaca tggcattggc
     5821 cgttgccatt gttggcagac caaaaaacag cccctgagcc ttccccgcag ctatcaacct
     5881 gtgcgccagg ataagcgccg cttctgtttt acctgcgcca gtcacatctt ccagaataaa
     5941 taactgcggt ccttgctggc tgatatccag atccagcacc ttttgctgta acggtgtcgg
     6001 attctcaata aaaggaaaca gcgtattaat tccgttgaaa ggcgcgactt ttgcttttag
     6061 aggaaagacg gttaaggcgt tttgagcctg aactaaagtt ttctgccagt aacctttaat
     6121 atccattggg tgtgcgacgc gtggaaaaaa tcgcgttgac gaacccgtcc agtctgcgag
     6181 cacgacggtt gcagagatat accaggaaag gtgttttata agttcaatgc cctcatcatc
     6241 atcccagaat gcggggatct ctatgagcgg aaacagtggt ttgatttcaa ggagaaaatc
     6301 tcgtgctgca gctttgtctt caggcagaaa attatccaat tcatccatac gatcaggggg
     6361 ccgaccatga tgccctgtcg tcacgggcat ccacatttct atcactcgcc taagtttgcg
     6421 cggagagagc gaagatgaag gaagtaactc ctgacattca cttaaataat gattccacaa
     6481 ccagtagccc agcgttgaat gagagatttt ttcgtaattt tttctggcac cttcctgaat
     6541 cttgagttca gggggcaggt aaagttgttg aaaagagcgg gcaaattttc caatatcgtg
     6601 ccagcacagc aaccaggcga aaaattgagc cgcctgttcc ttgtcagaaa tccctaattg
     6661 acgaaaataa tcagccagcc cgaagcaatt tcttttaacc attaaatagc ccatcgcggc
     6721 tacatccagc gaatgccagc aaagaaggtg atagtcgtcg cccccgtctt tctcgccgcg
     6781 tcgggttttt ccccagaaat caaaaaaagt cacaatattt ttatccttca gtaaacttaa
     6841 aggatattta cgcatatatt aaaaattatc tgtgatatat atcattcaat aaaatattta
     6901 cattatgaat aatcaaataa ttttcatccg taa
//
LOCUS       DAZPLI010000191         6825 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZPLI010000191 DAZPLI010000000
VERSION     DAZPLI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.192"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(155..667)
                     /locus_tag="ACHUU3_003796"
     CDS             complement(155..667)
                     /locus_tag="ACHUU3_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR2083759.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            862..1626
                     /locus_tag="ACHUU3_003797"
     CDS             862..1626
                     /locus_tag="ACHUU3_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083760.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1577..2980
                     /locus_tag="ACHUU3_003798"
     CDS             1577..2980
                     /locus_tag="ACHUU3_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR2083761.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            2980..4446
                     /locus_tag="ACHUU3_003799"
                     /pseudo
     CDS             2980..4446
                     /locus_tag="ACHUU3_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4337..5080
                     /locus_tag="ACHUU3_003800"
     CDS             4337..5080
                     /locus_tag="ACHUU3_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR2083762.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5084..6304
                     /locus_tag="ACHUU3_003801"
     CDS             5084..6304
                     /locus_tag="ACHUU3_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR2083763.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6308..6814
                     /locus_tag="ACHUU3_003802"
     CDS             6308..6814
                     /locus_tag="ACHUU3_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083764.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         1815 a         1622 c         1868 g         1520 t
ORIGIN      
        1 atcagcgacc agacagcatt gagaatgctt caggagtaca tcaggatcca atgccttggg
       61 gggtagcggt aattttactc attatccttc aaatcagatt ctgttgtcag aggaatgggg
      121 gaagctggat tcgcaagagc tttttatggg cagtttagct aaaaaaaggc aacacgaagc
      181 taaaactacc aacataaagt gctgaccagc tagcagctga aatcagactc actgtataca
      241 ctttcactgg atgaagttgc aacattcctg ccaccaaagg agcaatataa cgcagcacgg
      301 cgataaagcg cgaagtgaaa agtattgaca cagagttatt ttgcaactga aggcgaaccc
      361 gttctattgt attggaacgg cttgatatta tacatgcaag ttgagggata tgcccaatta
      421 ttataccaag atggtaatta acgatagtac cgcaccatgc gccactcatg accgtgattc
      481 cagcttccca tggtgaaaga gtcgtcttgc tgactgtaat tacagtggcc atcataattg
      541 atgctggtgg caatatcgcc gaaatgagta aagtggattt tgtgaacgct attataaaaa
      601 gaagccccca taggcgatat ggatgaagta caaaatagtt catcaaagca tttatccact
      661 ccatcaatgt ctttccttct gtgaattttt ctttgctatt taaccgtgat acgacttaat
      721 ttaggcttaa acaccttaca tttacaaggt actcctgtgg ggggccctgc cacggagcgt
      781 cgcaggagtg agtgatgagg aagaattaaa gcaacctatt gtgacaggca gcgataacag
      841 taaatcacag gaagcataat catggcaaaa ccggactggg aggccatcga atcggcatac
      901 cgggccggag tccttagcct ccgtgatata ggcgagaaat acggcgttac agaaggggct
      961 atcaggaaga gggccaaaaa gcttggttgg gcacgcagtg gcggtacgca ggtttgcaaa
     1021 aatggtacgc aaaaaaggaa agtgcgtacc agcagaaagc ctgccattac tggccttaca
     1081 caaaaaagta cgcaactaaa aacagaatct acaccggata cgaaaccgat acgcggaatg
     1141 cgtaccgatc ccccgactaa cccattccaa cccggtaacc agcaggcatt aaaacacggt
     1201 ggttatgccc gtcgccttct gctcaaagat gaggtgatag aggacgctaa agcgttgacg
     1261 ctcgaggacg aattatttcg ccttcgtgct aacaaccttg tcgctgcaga gaatattggt
     1321 cggtggttgg tgtcgctgga agatgctaat ggggaccagg aaaggaagat gctgattgaa
     1381 aatatcagcg ccgccgagaa agcaatgatg cgcaatacag ttcgtattga gtccatcgtt
     1441 ggcacgcttg cgacggtagg aaaaatattt gctgatacag cctaccgcaa ggccgccact
     1501 gataaggtgt ctctggaggc tgatcgtctt cgccgtgatg caggtattga tgatggcaat
     1561 ggagagcgtg acctcaatga cttctactct gacatccaaa ccgacgctga atccggcttt
     1621 acgtagtttc tggactatgc gggcacgtaa caaagtgctt tatggtggtc ggtcatcgtc
     1681 aaaatcatgg gatgccgctg gcattgccat atttctgtcg aataaataca ccctgcgttt
     1741 ttgttgtgcc cgtcagatcc agaataaaat cgaagagtcg gtgtataccc tgctcaaaat
     1801 tcagatagac aggtttggtc tgcggcaccg tttccgtatt ctgaacaaca aaatcattaa
     1861 ccgggttact ggctcggaat ttgtttttta tggattatgg cgcaacatcg aagaaattaa
     1921 gtcactggag gggatcgatg tgttgtggct ggaagaagcc cacgcactga cggaatacca
     1981 gtggaaaatt ctggagccaa cgatccgtaa agagggttcg gaatgctggt tcatattcaa
     2041 ccccggactt gttactgatt tcgtctggcg caacttcgtt gttgatccgc ccgaaggcac
     2101 tctcatccgc aaaattaact atgacgaaaa tccgtttctg tctgacacca tgcttaaggt
     2161 tatcgacgcg gcgcgacgcc gtgatccgga tggttttaaa catgtgtatg agggcgttcc
     2221 ggagtctgat gatgatgcgg caatcatcaa actgtcttgg atagaagccg cagtggatgc
     2281 gcacaaaacg ttaaatttcg aacccagtgg aagaaagcgt attggctttg acgtggctga
     2341 cagtggtaca gataagtgcg ctaacgttta ccgtcacgga tccgttgttt tctgggccga
     2401 cgaatggaag gccaaagaag atgaattact gaagagctgc cagcgtactt atcaggcggc
     2461 gctggagcgt gaagcagata ttgtttacga ctctatcggt gttggtgcgt ctgccggtgc
     2521 taaattctct gaaattaacg ctgaccggaa gagcgagaac gcatacgcgc gacgtgtgaa
     2581 ttaccagagg tttaacgccg gtgctggtgt gcatgagcca gatgacgagt acaacggcat
     2641 ccccaacaaa gactttttcg caaatcttaa ggctcaggca tggtggctgg tggctgaccg
     2701 tttcagaaat acgtttaacg ccattaacaa cggagaacag tatcctgtgg atgagctgat
     2761 cagcatagat tctcgttgtc cgttgcttga aaagctgaaa ctggaactga caacacctca
     2821 tcgtgatttc gaccgtaacg gacgtgtgat ggtcgaaagt aaaaaagacc tcgcaaaacg
     2881 cgagataccg tcaccaaacg ttgctgatgc attcattatg gccttcgcgc caatcgatac
     2941 atcgctggat atctgggaac agctggggag acaggcctga tggcacgaaa caaacaagcc
     3001 ctgcggcgaa ctgtgcaggc cacagctgat ggttatgaga attttattgc ccgcgtaggg
     3061 atgcagacac ctaaccagca ctcagcatcc acctaccggg ctaatttcac cagtcgtaac
     3121 cgcatgctgg tggaatggtc ctatcgttca tcctagatca tcggcgaagc agtcgatgct
     3181 atcccggatg atatgacccg caaaggcatt cgcatcactt cggaaattga tgcaaaagat
     3241 cgtggcattc tcgaatcaca actggatgag ttgcaaatct gggatgcgct gaatgacgtg
     3301 ctgaaatggt cgcgcctcta cggcggcgcg gtgggtttca tcatgattga ggggtaggca
     3361 ccaatgaccc cgctgcgacc cgaaaccatc ggtaagggca agtttaaggg gattctcccg
     3421 ctcgaccgct ggatggtcga cccggcactg acccgccgca ttaaagatat ggggccggac
     3481 ctgggtaaac ctgagtttta cgatgtggtg accacagcaa cgggaattcc tgcctggcgc
     3541 attcatcaca gtcgcctgat tcgctttgat ggcgtcacgt tgccatttca gcagaagatg
     3601 accgagaacg aatggggaat gtcggttgta gagcgtatct gggatcgtct taccgcgttc
     3661 gacagcgcta ctgtcggcgc ggcgcagctg gtctacaagg cgcatttgcg tacctacagc
     3721 gtggagaagc tacgcgagct tatcgcactt ggtggtcctg cgtatgaagc gttgctgaag
     3781 aatatcgacc tgattcgaca gttccagagt aatgaaggca tgacgctcat ggactcgcgg
     3841 gataagtttg aaacccatca gtacagcttc agtggtctgg atgacatcct ttcgcagttt
     3901 gtagaacaga ttagtggcgc tgttggtatc ccactggtgc ggttgttcgg acagtccccg
     3961 aaaggatttt ctaccggtga tgcagacctt gccaactatt acgatcgcat cagttcgttg
     4021 caggagaggc gtttacgtct tccggtgcgg cggatactgg acatcatgca tcgttcggaa
     4081 cttggcaagc cgctgccgga cgatttcacg tttgagttta acccgctctg gcaaatgtct
     4141 gatgtcgatc gctcaacggt ggcgttaaac accaccaacg caatcagtac ggcgctgggt
     4201 gatggtctga tgacactgaa agccgctatg actgatttgc gagaaaattc tgacgtaacc
     4261 ggcatcgggg catccattac cgacgaggac atcgagaatg ccgaagatga agcgccgccc
     4321 ggcatcggcg aacctgatga cgaaccgcag gagccgtcag gcggaaatcc ggtatcgaac
     4381 cagcctacgc aggatagcga gggcggtcgg agacatcgta aatggtcgct acgatggttc
     4441 aaatgacagt atcacggaaa ttattgcggc gctggagcgt tacagtgaaa tcatcacccc
     4501 ctgggcgaca aaggtcgcgg aaaactttac tgcggaccta acccggcaga acgagaaagt
     4561 ttggcggcaa cacagcaaga acatcagtcg cgagctccgc aatcttgtgg aaagcgctcc
     4621 tgtgggccag gtgatgcaat ccatcatcgc cgaacaggtc aagtacatca aatctctgcc
     4681 tctcgaggcc gcagacaggg tgtacgacat ccagaacaaa gcgatagagg ctgttgtcac
     4741 cggtggtcgg gcggagcagt ttgctaaaga gattgcatct accggcgatg ttgctaagtc
     4801 tagggccgat ctgattgccc gaacggaact gggaagagca acgggcgcgc tggatatggc
     4861 ccgagcgata gctattggct cggatggtta tatttggcgt acagccgatg atggcgatgt
     4921 ccgagattcc cacgatcaca tgaaaggtaa attcgtccgc tgggattcac ctccaacttt
     4981 ggacggcatg accggccacg cgggcgagct gccaaactgc cgctgctata aagagatcgt
     5041 gtttgttcgc gttccattcg caatgaaaag ggcagcataa cccatgaaat acttttttga
     5101 gaccaggctc ggggaaaccc gataccgcct ggctgacggc tcgttgctgt gcaaagacgt
     5161 gccgatagga cgaacaggta agcagctcta tggtgctgat gacctgccaa aactgaaacc
     5221 cgataagttc ggtgaaatag tcgtcacgcg ttctcctgag caggtattcc atccggccac
     5281 gcttgcctca ttcgaaggga tgagcatcac gatcctgcat cctgaagatg aaaacgggaa
     5341 tgtgcggctg gtaaatcccg agaactggaa agagcttgct gtcgggcacc tccagaatgt
     5401 ccggcgcggg acgggtgagc agtctgattt gatgctggct gaccttatcg tcaaagacga
     5461 aagcgccatt cagcttatcg aagatggcct gcgcgaagtg tcgtgcggct atgacgcgga
     5521 gtacgagcag accgagccag gtaaagccga gcaggtcgat attaccggaa accatgtggc
     5581 tcttgtccct aaaggcagag ccggaaatcg ttgtgcaatt ggagacagag acacaatggc
     5641 aaatcaaaag aaaaactggt ggaaccgcat gcgtgcggcc atcaagacag gagatgccga
     5701 caccatgaac gaactggtgg agtcggctcc cgcatcggtt acaggagatg agggggattt
     5761 gccgcagggc gttaatctca acatcaacct gtccccgcag caaccactac cggacaaagc
     5821 accagagatg ggcggagatc caaccggcga cagtgatgat gacctcaaaa cattactgaa
     5881 agccctgctg gctaagctgg aaagaaatgc cacgggcgat aacgataata agcctgacga
     5941 taatccgacc ggtgacggcg aggacgatga agaggaaacc acgattactg gtgactcagc
     6001 ctggcgtgcc gaagttatcg ttccgggtat cgatctgagc cgtaagatga aaccgaccgc
     6061 gttcaaacgc gaggttctgg cttctgctga caaaacgctg gttcgccaga tagtcggtga
     6121 tgcggatatc cgcaaattac cgaaacaatc ggtcgacatg gcgtttaatg ccgtgtctga
     6181 ggttgccaaa gggcgaaaca cccgcgccac caccggcgat gcacagcgcc taaacatggg
     6241 catgaccagt atcgcttccc tgaacaaaca aaacgctgaa ttctgggcaa accgtaaagg
     6301 gtaaaaaatg aataatgtat ttctgtaccg gatgcctgtt ggcattgccg gggctgtctc
     6361 tcgcccgcag gacttaaccg tcgaaccggt ggtccttaaa tccgataacg ccttcgctgc
     6421 ctatggcctg gctggtaaat acgatgatga cggttttttc gtgccgctgg cagatggtga
     6481 taccgcagac aaggtgaagg ggatctacgt gcgcccttat ccgaccacgt cgcagccgga
     6541 catggttcgc caggtgggaa caggcaagaa cttcccgggc gacgccatga agcgtggcta
     6601 cgtgaccgtt aatctcggtt ctgattttga tgccagcact atcaaaaaag gcgatccggt
     6661 atacgttgtc gtctccactg atgaatccat caaagtgccg ctgggtggat tcatgtccac
     6721 gtcagtcagt ggcaaaaacg tggtgctgac caacgctgaa ttcacaggtg ccggtgatgc
     6781 tgacggcaat gcagaaattt cctggaagat ttaaggaaca gacga
//
LOCUS       DAZPLI010000192         6790 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZPLI010000192 DAZPLI010000000
VERSION     DAZPLI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.194"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(239..724)
                     /gene="sixA"
                     /locus_tag="ACHUU3_003803"
     CDS             complement(239..724)
                     /gene="sixA"
                     /locus_tag="ACHUU3_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR2083765.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(927..3071)
                     /gene="fadJ"
                     /locus_tag="ACHUU3_003804"
     CDS             complement(927..3071)
                     /gene="fadJ"
                     /locus_tag="ACHUU3_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR2083766.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3071..4381)
                     /gene="fadI"
                     /locus_tag="ACHUU3_003805"
     CDS             complement(3071..4381)
                     /gene="fadI"
                     /locus_tag="ACHUU3_003805"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR2083767.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4561..4845)
                     /locus_tag="ACHUU3_003806"
     CDS             complement(4561..4845)
                     /locus_tag="ACHUU3_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR2083768.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5217..6557
                     /gene="fadL"
                     /locus_tag="ACHUU3_003807"
     CDS             5217..6557
                     /gene="fadL"
                     /locus_tag="ACHUU3_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR2083769.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYISWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1689 a         1818 c         1621 g         1662 t
ORIGIN      
        1 ttctctatta tctgtattaa taaatgacaa tgagtgtcaa acagacatta ttcatttcaa
       61 catgtaactt tgggcaaaat caagatacgc gcagaagaat aatttccgaa tgaaagtaga
      121 aagtgtttta tttatagatg gggagagtct tcgatagtag tgagattgac agatatccgc
      181 tgcctgatga gcaattatgc ccaatgaatc tacctcattg ggcataattt ggaactcatc
      241 agatagcttt tgccatcttc agattacacg ggctcatctg ccagttgaat gtgccgtttc
      301 cgctttcatc aagcgttacg ctggcaatgg ccgaggtggt aaacataggc ggcgtttcgc
      361 ccggacataa ctcggcaacc agatacccga ctaacggtaa gtgagagatc accagtaccg
      421 aagcaacacc ttcattggtc aacgcctgca aataggcact gaccagacca acatcgccgc
      481 agggcgttaa ctccggcaga acttctgcac tggaaggcag gttcagacaa tcccctactt
      541 cttccagtgt ttgctcggct cgcaggaacg ggctcaccag aacgcgttcg atttccactt
      601 tttgaccttt cagccagttc gccatcaggc gagattcgtc acaaccatta gtggtcagag
      661 gacgaaccga atcactggcg gcatcaaggg ctgcgtcgcc gtgacgcatg ataaaaactt
      721 gcatattgca ccgcttttgt taaccagatt cgtccgtttt ctttcatctg aaaccagaga
      781 aagaaaacgg tggccggcat tgtgccttat ccattcaccg aatgaaacgc tgttttttac
      841 ctcaatggcg taagtatagt caatatgcgt ttacattttg accaacactc cgccattcag
      901 cgcggattca tatagctttg accttcttat tgaaggtcag ttgcagttgt tttccaaaaa
      961 ctttccccac gcgcgcccat ctcgaccaaa cgctcgcaag gggtaaaacg ggaaccatac
     1021 tgcgtggcca gtcgttgcat tattgcaacc acttcgcccg cgccgagaga atcgatatag
     1081 cggaacggtc caccgagaaa tggcggaaaa ccaatgccaa ataccgcgcc aatatccccg
     1141 tcacgcacgc tacggataac ctgctcatca acacaacgta ctgcttcatt cagcatcaac
     1201 atcacacacc gttcagcaac ctgcggtgcg gagagtcgcc cctgcccttg tgcgccaatg
     1261 agcgggtaaa tggcgggatc gacctgtttt ttgcttttac gccctttctg accataaaga
     1321 tagaaacccc gaccattttt tctgcctttg cgatcgtcgt tcaaaattga agaaacaaca
     1381 tttgcaggcg cgctaaaacg ttctccataa gcggcttcca gtacaggaat aattttagtc
     1441 ccggtgtcga ttcctacctc atccaaaagt tggattgggc ctaccggaaa accaaatttc
     1501 actagcgcgg catcaatgtg ctctacccgt tcaccttcgg tcagcatgcg gatagcttca
     1561 ttaatgtaag gcgctaagat gcgattgacg taaaacccgg ctttgtcacg cacgacaatg
     1621 ggcgttttgc cctgtttttt cgccagtttt accgtagtgg cgatggtttg cgccgatgta
     1681 cccgcatgag gaataatctc taccagcggc attttttcca caggactgaa gaaatgcagg
     1741 ccaataactt gctcaggtcg cgcggcgtga gcggcgatat caccaatcgg taaagatgac
     1801 gtgttcgaag caaaaatggt atgagcggcg caattttgct ccacttccgc caccatctgt
     1861 tgttttaatt cgagattttc aaacaccgct tcaataatca gatcgcgatg ggcaaagccg
     1921 cgatagtccg tcgttccgga gattaatgcc agctgtttgt cacgttcgct ggctttgaga
     1981 tgacgacggc gaactttgcc ctccagctga tcccaactgt acttcagcgc atgatttatg
     2041 ccctgcgggt tgatatcttt aattctgacc ggaagccccg ctttacaagc agtgacataa
     2101 gcaataccgc cgcccatcaa gccaccacct aaaattccca cgctgtttaa tggcgcaggc
     2161 ggtgcatcac taccgggatc tttcttcacg tccgtactgg caaaaaagat actacgcaaa
     2221 gcttgcgatt gcggcgtcat cgccagttcg ccaaaggcgc gagcttcagc gtcatagccg
     2281 ctgctggtgc cctgcgctaa tcccgtttca acaacctcca ggatgcgttc tgtcgccgga
     2341 taattgcctt gagttttgtg ttctgttttc ttgccgacca ttttgaacag cagcgcacga
     2401 cctaacggcc ccgccagaat acgctcgcgt acaggtagag ggcgggaaga tgggcgatcc
     2461 ttcttcgcca gctcaacagc ggcttccagc aaaatggagt gcggaacaac gtcatccacc
     2521 agccccagct ttaatgcctg tttcgcccga agttgttttc cggtgagaat catctctaat
     2581 gctgtactga cgcctatcag acgcggtaaa cgctgggtgc cgcctgaacc gggtaacaat
     2641 ccaagttgta cttcaggcaa accgagcacc gttttaggat cgtcagtaca aacgcgaccg
     2701 tggcacgcca gcgccagctc cagcccaccg cccaggcaag cgccatgaat agccgcgata
     2761 accggaatgg gcaaagcatg aatctccgcc atcaactgtt ggccctgccg cgccagtgct
     2821 tccgcttctt gcgccgtttt gcagttgcca atcatgttga tgtctgctcc ggcgataaaa
     2881 ttgtccggtt tcgcggagat aaacaccacg ccgcgcaact ctttgttttc acggagttgc
     2941 ttaataatgg cgcgcacctg cgaggcaaat tccgccttca gggtattcat tttctcatcc
     3001 ggtacatcga tggtgatgat agcaatgttg tccagacgaa cattaagagt aaacgctgat
     3061 gccatttcca ttattccgcc tccagaacca ttgccgcgcc aagcccaccg gcagcacagg
     3121 cggtaactaa accaaatcca ccgccgcgac ggcgaagttc atgcaatgtc tgggtaatca
     3181 tccgcgcgcc ggtcgccgcg aagggatgcc cgtaagcaat cgaaccgcca agcacgttaa
     3241 atttgctatc gtccacttcg ccagtggcat gtgcacgccc cagtacttca cgagcaaaac
     3301 gttcactacc cagcaactga atattcgcca gtgtctgagc tgcaaaggct tcgtgcatat
     3361 cgatcaatgt cagttcgctc atcgtcaaac cggcacgttc cagcgccagc ggtgttgacc
     3421 aggctggacc gagcaacatg tcctgccaga catcaatcgc agtaaatgcg tagctgcgca
     3481 gataccccag cggcaccagc cctaattctt tcgcccggga ttcagtcatc aggatcaccg
     3541 ctgccgcgcc atcggtcagc ggcgtactgt ttgccgccgt taccgttccg tgtttgcgat
     3601 caaacgccgg gcgcagcttt gcgtaatcgg caagcgagga attaccgcga atattgttgt
     3661 cttcgacaag cggttgttta taaggaggga taaaggcagt catcacctct tctttgagtt
     3721 ttccgtctga ccatgcctga gcggcacgct gatgcgaacg gtgcgctaat gcatcttgct
     3781 gttctcgggt gatgccgtag gttttcgcca tttgttcggc ggtgtcgccc atccgcaagc
     3841 cagtagaata ttctgctacc gcaggcggta cgggcattaa gtcgcgcaaa cgcaggcgag
     3901 agaagagttt caggcgctgg ctcatggttc gagctttgtt gacatcaacc agcacgcgcg
     3961 ccagtttttt actgacgcca attggcaata cggaagagga atctgcccca ccggcaatcc
     4021 ccgctcggat agttcccgcc atcaggcttt ctgcgacgtt tgcaaccgcc tggaaactgg
     4081 tagcgcaagc gcggctgacg ctgtaagcat cggtatgcac attcattccg gtaccgagaa
     4141 caatttcacg cgcaatgttg ggggcttcag gcatttgtac gacctgacca aagaccagct
     4201 gttcaatcac ttcagcgggg atctcgctgc gtgccagcag ttcgcctacc accatcttcc
     4261 ctaaatcaac cgcgggaatg ccatgaaaag ccgtcgcctg gcgggcaaaa ggcgtacgta
     4321 aaccgctaac aatggcgata cgatcgccct ggcgggtaac cagcggtaaa acctgaccca
     4381 taacactccc ctgtaaaaaa ataaataaag tggtctgacc tgatcatagt cttaactatt
     4441 tttttacatt tagccaagcg gagaaaaagg aaagtgggag ctatgacaca gtgaagacag
     4501 gagaaagaaa agaaaacgcc cctgccagca actgacaggg gcgaacgtac ccgagaggaa
     4561 ttaacgcaga cccagctgga agatcagcat ttcagcttcg caggcaaaag tgaaatcgat
     4621 atccaggcgc acaccgtcag actcttcagt gaaagtcggg gagattttgc acggttcgga
     4681 ttccacgcta cgggcttttt cagtcagcgc cgccagcgtt tgttctgctt ctgcgcggtt
     4741 tgcaaacacg cggctgtaag acgcggtgca gtcggagttg tccataatgg tgccaacatc
     4801 catacagcag caaaccgggg tttcatcagc actacattta ctcatcgttg atttcctctg
     4861 tatgtgcacc caaggtgcca gataaacgtt gtggatattt tacgcttccg gaaagtgctg
     4921 ctccagttgt taattctgca aaatcggata agtgaccgaa atcacactta aaaatgatct
     4981 aaaacaaaat tcacccgaat ccgcgagtgc gcaacctcca aattttgcca gctggatcgc
     5041 gtttcttaga tcatatttga aaaaagatgg aaacatactt gcagcattcc agctggtccg
     5101 acctatactc tcgccactgg tctgatttct aagatgtacc tcagacccta cacttcgcgc
     5161 tcctgttaca gcacgtaaca tagtttgtat aaaaataaat cattgaggtt atggtcatga
     5221 gccagaaaac cctgtttaca aagtctgctc tcgcagtcgc agtggcactt atctccaccc
     5281 aggcctggtc ggcaggcttt cagttaaacg aattttcttc ctctggcctg ggccgggctt
     5341 attcagggga aggcgcaatt gccgatgatg caggtaacgt tagccgtaac cccgcattga
     5401 ttaccatgtt tgaccgcccg acattttctg cgggtgcggt ttatattgac ccggatgtga
     5461 atatcagcgg aacgtctccg tctggtcgta gcctgaaagc cgataacatc gcgcctacgg
     5521 catgggttcc gaacatgcac tttgttgcac cgattaacga ccaatttggt tggggcgctt
     5581 ctattacctc taactatggt ctggcaacag agtttaatga tacttatgca ggcggctctg
     5641 tcgggggtac aactgacctt gaaaccatga acctgaactt aagcggtgcg tatcgcttaa
     5701 ataatgcatg gagctttggt cttggtttca acgccgtcta cgctcgcgcg aaaattgaac
     5761 gtttcgcagg cgatctgggg cagttggttg ctggtcagat tatgcaatct cctgccgggc
     5821 aaactcctca agggcaagca ttggcagcta ccgccaacgg tatcgacagt aataccaaaa
     5881 tcgctcatct gaacggtaac cagtggggct ttggctggaa cgccggaatc ctgtatgaac
     5941 tggataaaaa taaccgctat gcactgacct accgttctga agtgaaaatt gacttcaaag
     6001 gtaactacag cagcgatctt aatcgtgcgt ttaataacta cggtttgcca attcctaccg
     6061 cgacaggtgg cgcaacgcaa tcgggttatc tgacgctgaa cctgcctgaa atgtgggaag
     6121 tgtcaggtta taaccgtgtt gatccacagt gggcgattca ctatagcctg gcttacatca
     6181 gctggagtca gttccagcag ctgaaagcga cctcaaccag tggcgacacg ctgttccaga
     6241 aacatgaagg ctttaaagat gcttaccgca tcgcgttggg taccacttat tactacgatg
     6301 ataactggac cttccgtacc ggtatcgcct ttgatgacag cccagttccg gctcagaatc
     6361 gttctatctc cattccggac caggaccgtt tctggctgag tgcaggtacg acttacgcat
     6421 ttaataaaga tgcttcagtc gacgttggtg tttcttatat gcacggtcag agcgtgaaaa
     6481 ttaacgaagg cccataccag ttcgagtctg aaggtaaagc ctggctgttc ggtactaact
     6541 ttaactacgc gttctgataa cgcgttcgcc tggataaagt cacctgcata gcaggtgact
     6601 ttaactcccc cacttcaccg aaagtagtgt cctcattgct tacctcctga gttttgcaaa
     6661 cagcctgttg gcagcttgcc ctttcaaatc aataagcggt atccacagac aaacctgaag
     6721 ggaaaggcat attttcaggc gttctgctcg tccttctcaa agagtttact tttctgcatt
     6781 tccaggatac
//
LOCUS       DAZPLI010000193         6768 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZPLI010000193 DAZPLI010000000
VERSION     DAZPLI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.195"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="ACHUU3_003808"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="ACHUU3_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR2083770.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            complement(1360..1518)
                     /locus_tag="ACHUU3_003809"
     CDS             complement(1360..1518)
                     /locus_tag="ACHUU3_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR2083771.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
     gene            2089..2238
                     /locus_tag="ACHUU3_003810"
     CDS             2089..2238
                     /locus_tag="ACHUU3_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083772.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(2983..3423)
                     /locus_tag="ACHUU3_003811"
     CDS             complement(2983..3423)
                     /locus_tag="ACHUU3_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR2083773.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3641..4030
                     /locus_tag="ACHUU3_003812"
     CDS             3641..4030
                     /locus_tag="ACHUU3_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083774.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4074..4478
                     /locus_tag="ACHUU3_003813"
                     /pseudo
     CDS             4074..4478
                     /locus_tag="ACHUU3_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4585..5421)
                     /locus_tag="ACHUU3_003814"
     CDS             complement(4585..5421)
                     /locus_tag="ACHUU3_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFR2083775.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5421..5696)
                     /locus_tag="ACHUU3_003815"
                     /pseudo
     CDS             complement(5421..5696)
                     /locus_tag="ACHUU3_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            complement(5771..6244)
                     /gene="dfrA14"
                     /locus_tag="ACHUU3_003816"
     CDS             complement(5771..6244)
                     /gene="dfrA14"
                     /locus_tag="ACHUU3_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HFR2083776.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            complement(6265..6768)
                     /locus_tag="ACHUU3_003817"
                     /pseudo
     CDS             complement(6265..6768)
                     /locus_tag="ACHUU3_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
BASE COUNT         1578 a         1753 c         1771 g         1666 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcat tccttcagct catacgtcac tttcatcctc
     2641 tgtgccattt gtacatcacc cccttcctga acgtgctgac agcgggtttt ccggctgcgc
     2701 cggcaacgct tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggatgggaa
     2761 gaacgccgga aacaggactt accccggctg aatggtgtga atttccgcgc tatgcacttg
     2821 tgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt
     2881 ggtttgttgc ctgttttacc ggtttcccgt cagaaacgcc ctgagggcct ctcaggcggt
     2941 gctgataaca gatgttatgg taaatcagag tcacctgcac tgttactctg aaccggagcg
     3001 aaacagccgc gaccagaatc cccgcttttt ttcctgtgcc agcgcgtcag ccagacggct
     3061 aatttcggcc tgtagttgtt ccctctcctc atttcgcagg cggttttctg cttcccgcgc
     3121 catcttgtct tcaagcatta gggtaacagt ctgctgcaga gcgtccagtt tctgctgaat
     3181 gacgtctagc tgagcatttt ccgcctggct aacgacagat ttggagtgtc cctccttgtg
     3241 cctttttggt gtctcagaag tgcgcagctc gccataggcc cgaatcagtt cactggtctc
     3301 gaactcccgg cgatcatttt ttcctgtgcg atagctgaca aggcctttag ccatgtcacg
     3361 atataattgc gagcgcgact ttcctgttaa tttcagtgtc tcttgaactg tgtgccatgc
     3421 cataaaagtg acagtgtctc ctgtgtgtac cgtattgtaa cagcagaaca tattactgtt
     3481 ttaacacaaa aggtctgagg ctgcccgtga ggcgtgaaaa atacacaaaa aacctgttga
     3541 cggtgtggtt ttgacgggcg tagtatttcg aatactgtat ataaacacat tgttataaat
     3601 acagtttttg ctgtttttaa aagtcaaaga ggataatttt atgagtacag agttaaatag
     3661 cggtactgtc ggtggttttg gtcacatggg accaaatgac ccctacaatg atggtcatag
     3721 cggctctgga agttcgggag gtagttttaa cggtggtggt aatggtggcc atgataatag
     3781 tagtggttca tcttatactg catcctttgg tctcatcact ggccgacacc cgataactgt
     3841 cccaggtacg aagaatatta attctgtcga tctttctgct gtcattaata acattataca
     3901 tcatgaaggt gcacgcaata acatgtacct agatactgcg aacaatgtaa gagtgggagt
     3961 gacgggcact ggctggcaat gtctagcaac ggcaggcatt tcggctgagg gtaaaagaac
     4021 tttccgctaa gcgatagact gtatgtaaac acagtattgc aaggacgcgg aacatgcctc
     4081 atgtggcggc caggacggcc agccgggatc gggatactgg tcgttaccag agccaccgac
     4141 ccgagcaaac ccttctctat cagatcgttg acgagtatta cccggcattc gctgcgctta
     4201 tggcagagca gggaaaggaa ttgccgggct atgtgcaacg ggaatttgaa gaatttctcc
     4261 aatgcgggcg gctggagcat ggctttctac gggttcgctg cgagtcttgc cacgccgagc
     4321 acctggtcgc tttcagctgt aagcgtcgcg gtttctgccc gagctgtggg gcgcggcgga
     4381 tggccgaaag tgccgccttg ctggttgatg aagtactgcc tgaacaaccc atgcgtcagt
     4441 gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg ggtcgtttgc gggaaggggc
     4501 ggaatcctac gctaaggctt tggccagcga tattctccgg tgagattgat gtgttcccag
     4561 gggataggag aagtcgcttg atatctagta tgacgtctgt cgcacctgct tgatcgcggc
     4621 cgcgatagct agatcgcgtt gctcctcttc tccatccgcg ttccaagctg cggaaaggca
     4681 cccataagcg tacgcctggt cgagcaggcg acgcggatcg acgtccagcg cacgagagaa
     4741 tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa aggtcgtctc tgtcagccgg
     4801 atcgtagaac atattggcgg cgccaaagcc cacttcaccg accagaccga cgggatctat
     4861 caccagccag ccgcgactgg agaacatgat gttttcatga tgcagatcgc catgtagccc
     4921 acgcagttcc gaggcattgc tcatcatttg atcggctata atcgccgcgt ggacgtagtc
     4981 agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga aacaaagctg caaagcgatc
     5041 ccggatcggg agaagggcag aaggcagggg ttcctcagat gcggcataca gcttcgccat
     5101 tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc tcggcaacga tgtgagagag
     5161 cattcgctcc ccggcatatt cgagcaacat cagattgttc tcacgaccga gcaaccggac
     5221 tgctcccctc ccattgcgcc ataccagata gtcggccccg cgcagttcat cagcaatgtc
     5281 ttctataggt ttcaatccct tgacgattgc aggagtcccg tctggcaatg aaactttcca
     5341 aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc gaaacgtgcc aatgagcagg
     5401 aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt ccaatcgcag atagaaggca
     5461 aggcgttcgc ggtcgggggc ttcgatcccc aatacattga ataggacagc gaaggcgcgc
     5521 tctgcttcat ctggcgctgc ccagttctct tcggcgttag caatcatgag tgccaaatcg
     5581 gcatagcgat ctgctgttcc gagccgccca aggtcgatca gacccgtgca ttgaagagtt
     5641 ttagggtcca ccatgaagtt cggcatgcag ggatcaccat ggcaaacaac catatcgtcg
     5701 ctatcaatcg cggaaaaagg cgtagtgcgc gaagcgaacg aagctttttg ccgtcgattg
     5761 catagctttg ttaacccttt ttccaaattt gatagcaata gttaatgttt gaagtaaagt
     5821 gttgctcaaa aacaacttcg aaggtatttg gaatactcgg gaagaaaaca tccccctctg
     5881 gctcgatgtc gatcgtcgat aagtggagcg tagaggccat gggtaatgtt tctcggtaaa
     5941 tttctccgcc accagacact ataacgtgac cggtgaattc agctagcctg tccatggcct
     6001 cttcgattga ctgaaataca actacattgt catcatttga tgtccaacct gagcgggtaa
     6061 cgaccgcgta tttcctattg gggagtgcgc ccatagattc aaacgtcttg cgacccacca
     6121 gaagccactg attgtaggtc aatgctttaa aaagtagctg ctcccctttc gcggaccagg
     6181 gtatgtctgg accgcaacca atcacgccgt ttttcgcttt cgcagctatc aatgatactt
     6241 tcaaggttct catcctgggt taacggtgcg ctcttggtcg agccgcaccg gtagctctcg
     6301 ttcgacacga gccaaaagat cgagctgcgg cgtactcttg tcctcgtccg gtaagaagtc
     6361 gggattgacg gcattgcggg acaccacatc aacggcgcgt ccgaacattc gcgacagcct
     6421 gcgctcaaac ggacattgat caaccgatag gctgtgaaca gcgccaagtt gctgccccat
     6481 tgacggccac gctttgagca aatccgctcc agacagatca gccgccggta ctcccggaat
     6541 tgccgttatc accaagcatg caccctcctg ttcctcctgc cagttgatga cctcggggca
     6601 agccacacct cgacctttga gccaaatgag gcggtcacgc tctccagcga gctcaccgcg
     6661 gcgggaagca ggtgcgattt tcgcgaaggc atgcccgtca ccacgtcgaa aaacaaaatc
     6721 accagattct ccgcctctga caggcaacca gtcagaatgc gattcacc
//
LOCUS       DAZPLI010000194         6661 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZPLI010000194 DAZPLI010000000
VERSION     DAZPLI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6661
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.196"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(78..290)
                     /locus_tag="ACHUU3_003818"
     CDS             complement(78..290)
                     /locus_tag="ACHUU3_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HFR2083777.1"
                     /translation="MLCFLIYITLPFSQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
     gene            complement(366..983)
                     /locus_tag="ACHUU3_003819"
     CDS             complement(366..983)
                     /locus_tag="ACHUU3_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFR2083778.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGAGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            1250..2749
                     /gene="ansP"
                     /locus_tag="ACHUU3_003820"
     CDS             1250..2749
                     /gene="ansP"
                     /locus_tag="ACHUU3_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFR2083779.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGMPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2864..3925)
                     /locus_tag="ACHUU3_003821"
     CDS             complement(2864..3925)
                     /locus_tag="ACHUU3_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFR2083780.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            4167..6269
                     /gene="pqqU"
                     /locus_tag="ACHUU3_003822"
     CDS             4167..6269
                     /gene="pqqU"
                     /locus_tag="ACHUU3_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFR2083781.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDIP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(6305..>6661)
                     /gene="mcbR"
                     /locus_tag="ACHUU3_003823"
     CDS             complement(6305..>6661)
                     /gene="mcbR"
                     /locus_tag="ACHUU3_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR2083782.1"
                     /translation="LTPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSN
                     MPILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLA
                     EIMQQNIAILYQQYNR"
BASE COUNT         1634 a         1570 c         1647 g         1810 t
ORIGIN      
        1 tagctctgac tcatcaagct ctccatattt gtcctataga taattgcgaa taaatacaga
       61 gaacgccatt tatcctttta gcatacctgc cggatatttt tatttgcatt aatattaatt
      121 gcatctccaa ctccgagcat atccatcatt aaaaactttt tgagattata gaatacctgt
      181 gataacaagt gaaacatctg cacaatgtga atagttagtt tcttttcaga ggaaatgaaa
      241 tagacaagct gactgaaggg gagagtgata taaatcaaaa aacataacat gaatttaatt
      301 aattacgccc atcattgtgg ataaagttac tgaataataa ataacataat cctctggtat
      361 cccactcaaa taatttcatt cctttttaat acttcttgta atttcggtaa ctgacataca
      421 gcatcagcaa tcgcactgat attcgtggca ttatcctgaa accattcatg cccagccccc
      481 catgtgcgca tagtgcaaag ataacagtcc accagcgtta gttgctcacc aaacgcataa
      541 ggttcagcgg tcaattgtga attcagccag atataaagcg atttacgata ttcaataaca
      601 ttcttcttta actgttcagg tgcgtcagga gcccaacgct cagggtaatc ggcgaaagtg
      661 aatgttggat agacattagc aaccagccag accaatagcc gttgaaactg ctgacgttcg
      721 gcgcgcccaa ccggtggcgc aaggtccggg cgacgatcga gaaccatcaa tgcaatcgcc
      781 gctgtctccg tcataatctc gtcattttcc agcgccagtg tcggaatctg acacaacggg
      841 ttcagggttt tcaataactc gcgtgaagtt ccctcgtggt caaaaccact gacatcaacg
      901 aactgataag gaatatcagc cagtgttagc atcagctcac tgattgtaga gccccatccg
      961 ggtacgccat acaccttaat catatgcccc tcacaatctt ttaaagatta agtgtaggac
     1021 agtacacagg ttaaccagca aaagtatttg tcattttttc gtcagttgta tgaatcccct
     1081 tcaggattgt aaggggatgt tttgcatcgt atatcgataa cgggtaggat gctgcgccga
     1141 tagttttata cccataaaga ataatggtga taactatcat cgccaggatg aataaacatt
     1201 gttcatggca acttatatga ctttttcatt aaagcaatca gggagagcaa tgagtaaaca
     1261 cgacaccgac acttcagatc aacacgccgc gaaacgccgc tggcttaatg cccacgaaga
     1321 ggggtatcac aaagcgatgg gcaatcgcca ggtacagatg atcgccattg gcggcgcgat
     1381 tggcaccggc ttgtttttag gtgcaggagc ccgactgcaa atggcgggtc cagcactggc
     1441 actggtttat ttaatttgtg gcttgttttc gttttttatt ctgcgtgcat tgggtgagct
     1501 ggtgctacac cgcccttcca gtggcagttt tgtctcttat gcccgtgagt ttttgggtga
     1561 gaaagccgct tatgttgctg gctggatgta cttcatcaac tgggcgatga cggggattgt
     1621 tgatattacc gccgtcgctc tgtatatgca ttactggggt gcgtttggcg gcgtgccgca
     1681 gtgggtcttt gcgctcgctg cacttaccat cgttggcacc atgaatatga tcggtgtgaa
     1741 atggtttgcg gagatggagt tctggtttgc gcttattaaa gtgctcgcca ttgtgacctt
     1801 tttggtcgtg ggtacagtgt tcctcggtag tggtcagccg ctggatggca acaccactgg
     1861 ctttcattta atcaccgata atggcggctt cttcccccac ggtttgctgc ctgcgctggt
     1921 gttgattcag ggcgtagtgt ttgcttttgc ctccattgaa atggtgggta cagctgccgg
     1981 agaatgtaaa gatccgcaga ccatggtgcc taaagccatt aacagtgtga tttggcgtat
     2041 tggcctgttt tacgtcggct ccgtggtgtt gctggttatg ttattgccgt ggagcgcgta
     2101 tcaggcgggg caaagtccgt tcgtgacgtt tttctctaaa ctgggtatgc catatatcgg
     2161 cagcattatg aacattgtgg tgctgaccgc tgccctctcc agcctgaact caggtctgta
     2221 ctgcaccggg cgtattctgc gctcgatggc gatgggcggt tccgcaccga gttttatggc
     2281 gaaaatgagt cgtcagcatg tgccgtatgc cgggattctg gcgacactgg ttgtgtatgt
     2341 cgtcggcgta ttcctcaact atctggtgcc gtcgcgcgta tttgagattg tgttgaactt
     2401 cgcgtcgctg ggaatcatcg cttcatgggc gtttatcatc gtgtgccaga tgcgcctgcg
     2461 taaagcgatt aaagaaggca aagcagcgga tgtcagtttt aaactgcctg gcgcgccctt
     2521 cacttcctgg ctgacattac tgtttttact gagtgtcctt gtgctgatgg cgttcgatta
     2581 cccgaacggg acttatacta tcgcggcgct gccgattatc ggtattctgc tggttatagg
     2641 ctggtttggt gtgcgcaaac gcgttgctga aattcacagc actgcgccag tcgtcgagga
     2701 agatgaagaa aaacaggaaa ttgtgtttaa gcctgaaacg gcgagctaat tgcaaaattg
     2761 cccggctacg tgccgggcaa atttagatta ctgacgaagg tgtgccgcat gcggcgtgat
     2821 aaaagtcagg caagtctctc cgttttcacc ctcgccatat ctattacagc gcaatacgaa
     2881 tcacatcgtc tggctgggta gcttcctgct gtttagtgga tttttgtttc acactgacat
     2941 acagcgtttt gccatcggca gacagcgcca ggctgtttgg atgagtcggc gtatcgaacg
     3001 ttttcactac tttatagctt ttcgcgtcaa tcacactgac tttacctgcc tgacgatgcg
     3061 ttacgtaggc ttcattacgc gctgggttaa acagcacagc cagtgattcc ggtgctgcaa
     3121 ccttcgccag aatattgcca ttacgggtat cgaccactaa cacttctgcg gctttagaat
     3181 cggtgataaa tgcacgctgc ctggcggtat caaggctaat gttgataaag aagtgctctt
     3241 tgccgtcatc cagcagcttt ttacggctga ggattttatt gtcggcggtg tcgatggtaa
     3301 tcaattcgcc gtcagcgtta gtggtgtaaa gacgtttgcc tttgctatca agcgccagac
     3361 cggtactcat tttaccggtg ttctggatgg cggttttcag tttgatattc tcgccatcaa
     3421 cgacccaaat cacgctctct ttaccaatac cactgatgta aacggtgttc gtggcatcgt
     3481 cagctaccag ctcacgcggt tgcagcgggc gcacctcttc cgtacgctta cgatcatcca
     3541 gcaccagacg gcctttcacc tcgcccgttt tggcatctat cgccgtgacc gcgctgttta
     3601 cagtgttacc aaaccacagt gtctgagtcg tgttattgat ggtgacacca aacggcttga
     3661 gatcgttatg gatcgcctgc gtcacttcca gagtgaccgg atcaagacga taaaccacgc
     3721 cgcctttatc cagtttgcgg ctttgcgaag tggcgagcca cagcgcgttt tcttgctggc
     3781 tataagccat ttcgtaggca cctttaccta ccgctttacg cagcatttct tctgcggcct
     3841 gcgtactgaa tgatgaagca acaagcaatg aacctaacag taatgaacca cgcaggcgcg
     3901 atgaaaacag atgacgtaaa tgcatgacga ctccctttga tgaaccgata tttttgctgc
     3961 ttcacaatgc ttcgctaagc gtaatcatcg ttacgctctt gttataaatg ggaatggtaa
     4021 tcattatttt ctcaaatgtg gagcatttct tgccgttacg actacaattt tctcaattac
     4081 ttaacgtatc gtgccgtttc gttttccatg aatttacaat tttattacca taacggcgtt
     4141 ttcccattct taacctggct tttttcatga agattttttc cgtccgacag accgttttgc
     4201 ccgcactact tgccctttcc cccgttgttt ttgccgctga tgaacagacc atgattgtca
     4261 gtgccgcacc gcaggtggtt tcagaactgg atatcccagc agcagtaagc gtggtggatg
     4321 gcgaggagat gcgcctggca acaccgcgca ttaacttgtc cgaatcactg accgacgtgc
     4381 ctggtttgca ggtacaaaac cggcagaact atgcgcaaga tttacagctg tcgattcgcg
     4441 gatttggctc ccgctccact tacggtattc gcggtattcg cctgtatgtg gacggtattc
     4501 ccgccaccat gcccgacggg caagggcaaa catccaacat cgatttaagc agtgtgcaaa
     4561 atgtggaagt gctgcgtggc cccttctctg ccctgtatgg caacgcgtct ggtggggtaa
     4621 tgaatgtcac cacccagacc ggacaacagc caccaaccat tgaagccagt agttactacg
     4681 gcagttttgg cagctggcgc tatgggctga aagcaacggg cgcaacggga gacggcacac
     4741 agcctggcga tgtcgattac accgtctcaa ccacgcgttt tacgacccac ggctatcgtg
     4801 accatagtgg cgcacagaaa aatttagcca atgccaaact gggcgtacgc attgatgaag
     4861 ccagcaaatt aagtctgatt ttcaatagtg tggatatcaa agcagatgac ccgggtgggc
     4921 taaccaaagc agaatggaag gctaatccac aacaagcgcc tcgtgcagaa cagtacgaca
     4981 cgcgaaaaac catcaagcaa actcaggctg ggttgcgcta tgagcgtagc ctgagcgcgc
     5041 aagatgatat gagtgtgatg atgtatgccg gagagcgaga aacgacccag taccagtcaa
     5101 tacccatggc accacaactt aacccgtcac atgcgggcgg cgtgattacc ctgcaacgcc
     5161 attaccaggg gatagacagc cgctggacac accgtggtga actgggcgtt ccggtcacgt
     5221 tcactaccgg cctgaactac gaaaacatga gtgaaaaccg caagggctac aataacttcc
     5281 gcctgaatag cggcatgccg gagtacgggc aaaaaggtga gttgcgtcgc gacgaacgca
     5341 atctgatgtg gaacatcgat ccctatttac agacgcagtg gcagctgagc gaaaaactgt
     5401 cgctggatgc tggcgtgcgc tacagctccg tgtggtttga ttccaacgac cattacgtta
     5461 ctccgggtaa cggcgatgac agcggtgatg ccagttatca taaatggcta cctgccggtt
     5521 cgttaaaata tgcaatgacc gatgcctgga atatctatct ggcagccggg cgaggttttg
     5581 aaacgccgac gattaatgag ctgtcttatc gtgctgatgg gcaaagcggt atgaactttg
     5641 gtttaaaacc atccaccaac gatacaattg agatcggcag taaaacgcgt attggtgatg
     5701 ggctgcttag tctcgcattg tttcagaccg acactgatga tgaaattgtt gtcgatagca
     5761 gtagcggtgg gcgtacgact tacaaaaatg ccggaaagac ccgtcgtcaa ggcgctgaac
     5821 tggcatggga tcaacgtttc gcaggagatt ttcgcgtaaa cgcgtcctgg acctggcttg
     5881 atgcgaccta tcgcagcaat gtttgcaatg aacaggattg taacggtaat cggatgccag
     5941 ggatcgcccg taatatgggc tttgcgtcga taggttatgt accggaagat ggttggtatg
     6001 caggcacgga agcgcgttat atgggcgata ttatggcaga tgatgaaaat acggcaaaag
     6061 cgccgtctta tactctcgtc ggcttattca ccgggtataa atacaattac cacaatttaa
     6121 ctgtggattt atttggtcgt gtcgataatt tattcgataa agaatacgtt ggttctgtca
     6181 ttgtcaatga gtcaaacggg cgatattacg aaccttcgcc cggacgaaat tatggtgtcg
     6241 gcatgaatat tgcgtggaga tttgagtaat taccttgatg cccggtattt gccgggcatt
     6301 tactttaacg attgtattgc tggtataaaa tagcaatatt ttgttgcata atttcagcaa
     6361 gacaatgtct gcatccctct ttgtcttttg cttttaacgc agcgagtaac agatgatagt
     6421 tttctatatg ctcccgtaat tccgctggat taatcgcttc atagagataa tgtaaaccag
     6481 gccccatcct gacccacagt tgctcaatca tctcacacag gatgggcata tttgagcgat
     6541 gataaatcgc taagcgaaat agcctgttta cattaatgat ttgttccatg tcgccctttt
     6601 cttgcgcctg ctgtaatttc tcgagcaatt cctggagttc cgtcaggtct tgcggggtga
     6661 g
//
LOCUS       DAZPLI010000195         6660 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZPLI010000195 DAZPLI010000000
VERSION     DAZPLI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.197"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHUU3_003824"
     CDS             <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHUU3_003824"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFR2083783.1"
                     /translation="FIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQKADSSFLYDK
                     YQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQT
                     ASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEP
                     VNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYV
                     AGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQY
                     VSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSREN
                     ISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAI
                     TLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIG
                     FSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            1506..2009
                     /gene="yeaK"
                     /locus_tag="ACHUU3_003825"
     CDS             1506..2009
                     /gene="yeaK"
                     /locus_tag="ACHUU3_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR2083784.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(2010..2114)
                     /gene="yoaI"
                     /locus_tag="ACHUU3_003826"
     CDS             complement(2010..2114)
                     /gene="yoaI"
                     /locus_tag="ACHUU3_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR2083785.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2284..2730
                     /locus_tag="ACHUU3_003827"
     CDS             2284..2730
                     /locus_tag="ACHUU3_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR2083786.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="ACHUU3_003828"
     CDS             complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="ACHUU3_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFR2083787.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3605..4786
                     /gene="nimT"
                     /locus_tag="ACHUU3_003829"
     CDS             3605..4786
                     /gene="nimT"
                     /locus_tag="ACHUU3_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFR2083788.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4841..5188
                     /locus_tag="ACHUU3_003830"
     CDS             4841..5188
                     /locus_tag="ACHUU3_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR2083789.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="ACHUU3_003831"
     CDS             complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="ACHUU3_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR2083790.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5647..>6660
                     /gene="dgcP"
                     /locus_tag="ACHUU3_003832"
     CDS             5647..>6660
                     /gene="dgcP"
                     /locus_tag="ACHUU3_003832"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR2083791.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSA"
BASE COUNT         1616 a         1632 c         1615 g         1797 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     1081 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
     1141 atgacaccct cggtcatcag gagggggatt tagcgattac gttattagct caggcgatta
     1201 aacaatcgat tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     1261 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     1321 tgcaacatat cgcgccgcag aaagaaatcg gcttctcttc cggtatttac gcgatgaaag
     1381 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     1441 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     1501 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     1561 cacaagaagg tgctaacttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     1621 tgtcagaaat tcgtggcacc gctttaggcc agggagctaa ggcactggta tgtaaagtca
     1681 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc tgccgatcaa caggccgatc
     1741 tgagccaact tgccagccat ataggcggat tacgcgcttc gttagccagc cctgctgaag
     1801 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     1861 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     1921 ggatgctgga taaatccgtt attttgaaaa ccacggatta tttgcgcatt gcgcaaccag
     1981 aactgctcaa tttccgccgc actgcgtaac tagcctgtcc gttcaataag cagaacggac
     2041 aaaaccagca caacagcaat ggcaaaaaac gatgacgtga taatcagtgt ctcgacgaac
     2101 atttgatcgt tcatagcatg cccccgggag atagctgttt tcacgttatt gttagcgtgc
     2161 acaaatggca gtttgatgac aattcgcctg attttttatg caaaaaacgt aaagattttc
     2221 tacttccttc ctgcagcaaa cgtaaagtaa gcaggcttat tattttttgg caaggaaacc
     2281 acgatgtttg atgtcacttt gctgatcctg ctcgggttag ctgcgctggg ctttatcagt
     2341 cataacacca ctgtcgccgt ttcaattctg gtgttgatca ttgtccgcgt cacaccgtta
     2401 agcacctttt tcccgtggat tgaaaaacag gggctaagta tcgggataat cattctgacg
     2461 attggcgtta tggcccccat cgccagcggg acgctaccgc cctcaacctt gattcactcg
     2521 tttctgaact ggaaatcgct ggtggcaatt gcagtaggcg tgattgtctc ctggctgggt
     2581 gggcgcggcg tgaccttaat gggcagccag ccgcaactgg tcgccgggct gctggtaggc
     2641 actgttttag gcgtggcgct gtttcgcggc gtaccggtcg gcccgcttat tgccgcgggt
     2701 ctggtttcgc tgattgtggg gaaacagtag ttaatcccgc aatatatcgg cctggcgttt
     2761 gccccaggcc tttcttaaac atagtaataa aggccgttgt tgaatcatac cccagcgtat
     2821 gagcaacttt ctgtacagta tcgcctttca ctaatccctg aagtgccata atcagctgta
     2881 actgctgacg ccactggcga aaactcaacc ccgtctcttt tactatcagt cgcgcaagat
     2941 tgcgttcact catcgcaaaa aagccagccc attgccctaa tgacccccac tcgacgggcc
     3001 ctttcgccat catctccacc atcgtgcgga ttttaggatg agaagaaacg ggcaggtgca
     3061 gctgttgttg tggctgctga gggagttcat caaacaggac ctgaattaat ctgcgggtca
     3121 tcggctcagc acgctgagtc gtcgtcctgt ttgccagggt taaaatcaat tcccggcata
     3181 agggcgatat tttcaatgtg caacatgttg tcggcattgt cactgcgctg ggttcgataa
     3241 acagaaaaca gagttcagcg ttagctgtca cttgattgct atgttccacc ccgcccggta
     3301 tccaaaccgc atattgcggc ggcaccatcc acaaagcatt ttccaccgta caggtaattg
     3361 caccatgtaa tgctagtatc agctgtcctt tgcggtgctg atgtacagga ctggttaact
     3421 catccgttcc ggcgtgaata caaaatgcga ccgcggcttc atggtgtcgg tcgggttcat
     3481 agccattgag attcaacctg tgcatcattt tgtccgaatt tagcgataat ttgtcatttt
     3541 agcttgattc aacataacaa taaaaacggt aaggtacagc ctcgtttgta acaatgagaa
     3601 gcatatgacc tgttcaactt cattaagcgg caaaaacagg attgtcctta tcgctggcat
     3661 tctgatgatt gccacaacat tacgcgtcac ctttatcggc gcagcaccgt tactggatac
     3721 gattcgttcc gcttactcgc tgacgacagc gcaaaccggc ttattgacca ccctgccatt
     3781 attggccttt gcgctaatct cacctttggc caccccggta gcgcgacgtt ttggtatgga
     3841 acgtagcctg tttgccgcgt tacttttgat ctgtgctggt atcgcaattc gctctctccc
     3901 ttcgccttac ttattatttg gcggtacagc ggtcattggc ggtgggattg cattaggcaa
     3961 tgtcttactg ccaggattaa ttaaacgcga tttccctcat tccgtcgcca gacttaccgg
     4021 cgcatattcc ctgacaatgg gagctgcagc ggcactggga tcggctatga tagtgccgct
     4081 ggctttgaac ggttttggct ggcaaggcgc gttgctcatg ctgatgtgtt ttcctctgct
     4141 ggctcttttt ttatggctgc cacagtggcg aagccaacaa catgcaaatt tgagtacctc
     4201 gcgcgcctta catactcggg gtatctggcg ttcgccgctt gcctggcagg taacattgtt
     4261 tcttgggatc aactcactga tctattacgt gattattggc tggcttccgg cgatcctcat
     4321 cagtcacggc tatagcgaag cacaggcggg ttcactgcat ggtttgctgc aactagccac
     4381 agcagctccc ggtttgctga tccctctttt cttacatcat gtgaaagatc agcgtggtat
     4441 tgcagcgttc gttgccttga tgtgcgcagt gggcgctgtt gggctctgct ttatgccagc
     4501 gcacgcgatc acctggactc tgcttttcgg ttttggttcc ggcgcaacaa tgatactggg
     4561 gttgacgttc attggtcttc gggcaagttc tgcgcatcag gcggcggcac tctcggggat
     4621 ggcacaatcc gtcgggtatt tgctggcagc ctgtgggccg ccgctaatgg gtaaaataca
     4681 cgatgctaac ggtgactggt ccgtaccact gatgggtgtt gcaatacttt cactactgat
     4741 ggcgattttc ggactttgcg ccgggagaga caaagaaatt cgctaatatc cggtgctata
     4801 gtgacgtgac aaatcatgcg tgaaagggag aacaaacacg atgaatattc agtgcaaacg
     4861 cgtttatgat ccggctgaac agagcgatgg ttatcgcgta ctggtcgacc gcctctggcc
     4921 gcgcggtatc aaaaaaaccg atttagccct tgatgagtgg gataaagaaa tcacgccgtc
     4981 aacggaactg cgcaaagcct ttcacggcga agtcgtcgat tttgcaacct ttcgcgagca
     5041 atatcttgca gaactggcgc aacacgagca agaaggaaag cggctggcgg acatcgccaa
     5101 aaaacagccg ctgaccctgc tctacgcagc aaaaaacacc acgcagaacc atgcgctggt
     5161 gctggccgac tggctacgta gcttgtgatt ttagtacagc atccggcggt tatttttcac
     5221 cagccggatg gtcccgtcgc cacaatgccc attcatcaat cgtttcaccg cccggtaatt
     5281 tgcaattgtt gctgacccct tgcgctgtcg acactggaat gagcgtcccg cccttctgct
     5341 ggcaatagac cgacgccgga tttgccatac caatctgcgg cggtttaggt acttctggct
     5401 gagaagaggt tgaacaacca gccaggacca gcaagcaagg caaagcaaaa ctgataattt
     5461 tcatttattg atctcactca tttatctacg cttagagtat cgcggtatcg ttttgttttg
     5521 cagcactatt tttattacat tcactcaaaa catattacgt cttgtttcat ctttgttggt
     5581 gatgttttat catgcctgca aagattaaat aatcagcatt tacccgccgt atcctggagt
     5641 tgtttcgtgt cagatcagat tatcgcccgc gtctcgcaat cccttgccaa agaacagtca
     5701 ctggaaagtc tggtccgaca gcttctggag atgctggaaa tggtcactga tatggaatca
     5761 acctacctga ccaaagtgga tgtcgaagcg cgcctgcagc atattatgtt tgcccgtaac
     5821 agccagaaaa tgcacatccc ggagaatttt accgtctcgt gggattactc gttatgcaaa
     5881 cgcgccattg atgaaaactg ctttttcagc gatgaagtcc ctgaccgttg gggcgactgt
     5941 attgcggcac gcaatcttgg catctccaca tttctgagca caccaattca cttaccggat
     6001 ggatcattct atggcacgct ttgcgccgcc agcagtgaga agcgccagtg gagtgaacgc
     6061 gcggaacagg ttttacagtt attcgccgga ctgattgcac aatatattca aaaagaggca
     6121 ctggttgaac agttgcgcga agccaatgct gcgctgattg cgcaatcgta taccgactcg
     6181 ttaaccgggt tgccgaatcc gcgggcgatt tttgaaaatc tgacgacgct gttttctctc
     6241 gcccggcatc ttaaccataa gataatgatc gcgtttatcg atctggataa cttcaaatta
     6301 atcaatgatc gttttggtca taatagtggc gatctgtttc tcattcaggt tggcgagcgc
     6361 cttaatacgc tccagcaaaa tggcgaagtt attggtcgtc tcggcggtga tgagtttttg
     6421 gttgtttcac taaacaacga gaatgcggat atttcgtcgc tgcgagaacg cattcagcag
     6481 caaatacgtg gagaatatca cttaggtgat gttgatttgt attatcccgg tgccagtctt
     6541 ggcatagtag aagtcgatcc tgaaacaacc gatgcagaca gtgccctgca tgctgccgat
     6601 attgcgatgt atcaggagaa aaaacacaaa cagaaaacac cttttgtcgc gcattcagcg
//
LOCUS       DAZPLI010000196         6591 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZPLI010000196 DAZPLI010000000
VERSION     DAZPLI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.198"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            128..904
                     /gene="exuR"
                     /locus_tag="ACHUU3_003833"
     CDS             128..904
                     /gene="exuR"
                     /locus_tag="ACHUU3_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR2083792.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1144..1227
                     /gene="yqiM"
                     /locus_tag="ACHUU3_003834"
     CDS             1144..1227
                     /gene="yqiM"
                     /locus_tag="ACHUU3_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR2083793.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1249..1911
                     /gene="yqjA"
                     /locus_tag="ACHUU3_003835"
     CDS             1249..1911
                     /gene="yqjA"
                     /locus_tag="ACHUU3_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR2083794.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1915..2298
                     /gene="mzrA"
                     /locus_tag="ACHUU3_003836"
     CDS             1915..2298
                     /gene="mzrA"
                     /locus_tag="ACHUU3_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR2083795.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2445..2813
                     /gene="yqjC"
                     /locus_tag="ACHUU3_003837"
     CDS             2445..2813
                     /gene="yqjC"
                     /locus_tag="ACHUU3_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFR2083796.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2851..3156
                     /locus_tag="ACHUU3_003838"
     CDS             2851..3156
                     /locus_tag="ACHUU3_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR2083797.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3159..3563
                     /locus_tag="ACHUU3_003839"
     CDS             3159..3563
                     /locus_tag="ACHUU3_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR2083798.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3553..3852
                     /locus_tag="ACHUU3_003840"
     CDS             3553..3852
                     /locus_tag="ACHUU3_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR2083799.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4038..4430
                     /locus_tag="ACHUU3_003841"
     CDS             4038..4430
                     /locus_tag="ACHUU3_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR2083800.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4500..5485
                     /gene="yqjG"
                     /locus_tag="ACHUU3_003842"
                     /pseudo
     CDS             4500..5485
                     /gene="yqjG"
                     /locus_tag="ACHUU3_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
     gene            5778..6143
                     /locus_tag="ACHUU3_003843"
     CDS             5778..6143
                     /locus_tag="ACHUU3_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR2083801.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            6385..>6591
                     /locus_tag="ACHUU3_003844"
     CDS             6385..>6591
                     /locus_tag="ACHUU3_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR2083802.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIR"
BASE COUNT         1681 a         1593 c         1607 g         1710 t
ORIGIN      
        1 atttataaat attcacaata ttaattcaat acattaattc aatcaccaaa tttatatatg
       61 accaatggca tacccatgaa taaagtggta tgataacttc gtccgcattc cactccggga
      121 gacgcatatg gaaatcactg aaccacgccg tttgtatcaa caacttgccg ctgacctgaa
      181 agagcgcatt gaacagggcg tctatctggt gggtgataaa ctgcctgccg aacgctttat
      241 tgccgatgaa aagaacgtca gccgaacggt agttcgtgaa gccatcatca tgctggaagt
      301 tgaaggctat gtggaggtgc gtaaaggttc aggtattcat gtggtttcca accagccgcg
      361 ccatcagcag gcggctgaca ataatatgga attcgccaat tacggtccgt ttgagttgct
      421 tcaggctcgc cagctcatcg aaagtaatat tgccgagttc gcggcaactc aggtaacgaa
      481 acaagacatc atgaaactga tggccattca ggaacaggcg cgcggcgaac aatgcttccg
      541 tgattccgag tgggatttac agttccacat tcaggtagcc ctggcgacgc agaactccgc
      601 cctggcggcc atcgttgaaa aaatgtggac ccagcgtagc cataacccgt actggaaaaa
      661 actacacgaa cacattgatt cccgtaccgt tgataactgg tgtgatgacc acgatcaaat
      721 tctcaaggcg ttaattcgca aagatcccca tgctgctaag ctggcaatgt ggcagcatct
      781 ggaaaacacc aaaatcatgt tatttaacga aaccagcgac gatttcgagt tcaatgccga
      841 ccgctatcta ttcgccgaaa acccggtagt gcatctcgat acggccacca gtggcagtaa
      901 atgaaactcc tgtccgacag gcgctgcgac gcgcctgttc gcttcgtcgg taagcgaaaa
      961 gtataaagtg tcagcctgtg taaatcctct cgccaccctc ccctgcattc agcaaaatca
     1021 gactccacgg acatgtaatt ttgataacga acaacgttga cctttgttac aattagattc
     1081 aatttgaatt tatgtttttg aatgctttct tatctcacga tttaacaggg aatagttcag
     1141 gctgtgttga tgtatcaaac ccgcagaaca taccaaaaca gcaataacat tgcggtagtg
     1201 catcttttaa aaccagcgtg gcgttaaccg attcaccagg aataatgaat ggaacttttg
     1261 acccaattgc tgcaagccct gtgggcgcag gattttgaaa ccctggccaa tccatcgatg
     1321 attggcatgt tgtattttgt cttgtttgta attttgttcc ttgaaaacgg cttgcttcca
     1381 gcggcctttt taccgggcga cagtttactg gtattggtcg gcgtgttgat tgcgaaaggc
     1441 gcgatgggtt acccgcaaac gattctgctg ctgaccgttg ccgccagcct cggctgctgg
     1501 gtcagctata ttcaggggcg atggctgggc aatacccgca ccgtacaaaa ctggctatct
     1561 catttacccg cgcattatca tcaacgcgca caccatcttt ttcataaaca cggtttatcg
     1621 gcgctgttaa ttggtcgctt tattgcgttt gtcagaacac tgctgccgac gattgccggg
     1681 ttatcagggc tgaataacgc gcgctttcag tttttcaact ggatgagcgg tctgctgtgg
     1741 gtattgatcc tgacaactct gggttacatg ctcggcaaaa cgccgatatt tttaaagtac
     1801 gaggaccagc tgatgtcatg cctgatgctg ctcccggtgg tgctgctggt gtttggcctg
     1861 gcaggttctc tggtcgtgtt atggaaaaag aaatatggaa atcgggggta agggatgcaa
     1921 atacctcgca tgtcgcttcg ccagctagcc tggtccggca ctgttttact tctggtcggc
     1981 acgctgttgc tggcctggtc cgcggttcgc cagcaagagt ctacgctggc gattcgtgcc
     2041 gttcatcaag gcacaacgat gccagacggt ttttcgatct ggcatcacct tgacgctcat
     2101 ggcattcctt tcaaaagtat cacccccaaa aacgacactc tgttaattac atttgactcc
     2161 agcgaccaga gcgccgccgc aaaagcggtc ctcgacagaa cattgcccca tggctacatc
     2221 attgcgcagc aggacaataa cagtcaggct atgcagtggc tgacccggtt acgggataac
     2281 tctcatcgct tcggataact tcctgttatc cgaaacatat cactcacttt ggtgatttca
     2341 ccgtaactgt ctatgattaa tgaggcggtg gttaacacta ccgcttcgtt gtctggacaa
     2401 tcgttccttt gtaataggtc cgaatcacaa tggaaggttc aagaatgaaa taccgcatcg
     2461 ctttagctgt ttctctcttt gctcttagtg ccggtagtta tgccactacc ctgtgtcagg
     2521 aaaaggagca aaatatcctt aaggagatca gctatgccga aaaacaccaa aaccagaatc
     2581 gtattgacgg tctgaataaa gccctgagtg aagtccgggc caactgttca gatagccagc
     2641 tgcgtgccga tcatcacaag aaaatcgcaa agcagaaaga tgaggtggcg gaacgccagc
     2701 aagatttagc cgaggcgaag caaaaaggcg atgccgataa gattgccaaa cgcgaacgga
     2761 aactggcaga agcgcaggaa gagctgaaaa agctggaagc gcgcgactac taactcacaa
     2821 tagtcactac ttactcacct ggagaaaact atgtcgaaag aacacactac ggaacatctg
     2881 cgtgctgagt tgaaatccct ttccgatacg ctggaagagg tgcttagctc atctggcgag
     2941 aagtcgaaag aagagttgag taagattcgt agcaaagcgg agcaggcact gaaacagagc
     3001 cgttatcgcc tgggtgaaac cggtgatgcc atcgccaaac aaacccgtgt cgcggcggcg
     3061 cgtgctgatg agtatgtgcg tgaaaatccg tggaccggcg tgggaattgg cgctgcaatc
     3121 ggtgtagtgc tcggcgttct gctgtcgcgt cgttaattat ggcggacact catcacgcgc
     3181 aagggcccgg taaaagcgtt ctgggcatcg ggcagcgaat tgtttccatc atggttgaaa
     3241 tggtggaaac acgtctgcgg ctggcggtgg tggagctgga agaggaaaaa gctaatctct
     3301 ttcaactttt actgatgctg ggcctgacga tgcttttcgc tgcatttggt cttatgagcc
     3361 tgatggtgct aattatttgg gcggttgatc cgcaatatcg cctgaatgcg atgattgcca
     3421 ccaccgtggt gttgctgcta ctggcactga ttggcggtat ctggacgtta cgtaaatcgc
     3481 gtaaatccac cttgctgcgc catacacgcc atgagctggc aaacgatcgg caactgctcg
     3541 aggaggagtc ccgtgagcag taaagtcgaa cgtgaacgac gtaaggcgca actgcttagc
     3601 cagatccagc aacaacggct ggatctttcc gccagtcgtc gtgaatggct ggaggcaaca
     3661 ggcgcttacg atcgtcgctg gaatatgctg ctaagtctgc gctcctgggc gctggttggc
     3721 agtagcgtga tggcgatctg gacgattcgc catcctaata tgctggtccg ctgggccaga
     3781 cgcggttttg gcgtatggag cgcctggcgt ctggttaaaa cgaccctcaa gcagcaacag
     3841 cttcgcggtt aacttccctc tggccggagc aattccggcc ttatccctca aattttttga
     3901 agatttttga cagttttcct tgctaacaat catcattcac cacgtttatg attctctcca
     3961 tcgacagcaa cgacgctaat accgcgccct tgcacaaaaa aacaatcagc agcctgagtg
     4021 gcgcagtgga gagtatgatg aaaaaattag aagatgttgg tgtactggta gcgcgcattt
     4081 taatgccgat tctgtttatt accgcaggct ggggaaaaat caccgcttac tctggtaccc
     4141 aacaatatat ggaagcaatg ggtgtcccgg gttttatgct gccgctggtg attctgcttg
     4201 agttcggtgg tggtctggca atcctgttcg gtttcctgac tcgcaccaca gccctgttta
     4261 ctgcgggctt tacgctgctg acggcatttt tatttcacag caactttgct gaaggcgtca
     4321 actcgctgat gttcatgaaa aacctgacaa tttctggcgg attcctgctg ctggcaatta
     4381 ccggtccggg cgcgtatagc atcgaccgcc tgctgaataa aaagtggtaa gcacgcgcta
     4441 tacttaacga taaaaaacga ggaggaagct cctcgttttt gctattggag gagagaaaaa
     4501 tgggtcaact gattgacggc gtctggcatg acacctggta cgataccaaa tctaccggcg
     4561 gtaaatttca acgttcagct tccgcatttc gtaactggct cactgccgat ggcgcacctg
     4621 gccccactgg cacaggcggt tttatcgcag agaaagatcg ttatcatctc tatatttcac
     4681 tcgcctgccc gtgggcgcac cgcacgctga tcatgcgcaa actcaaagga ctggaaccgt
     4741 ttatttccgt ttccgtagtg aacccgctga tgctggaaaa cggctggacc tttgatgaca
     4801 gttttccggg agcaaccgga gatacgctct atcaacatga atttctgtat cagctttatc
     4861 tccacgccga tccacactac agcggacgag ttactgttcc cgtactgtgg gacaaaaaga
     4921 accacaccat cgtcagcaac gaatcaacag aaatcatccg catgttcaat accgcgtttg
     4981 atgcgctggg cgcgaaagcg ggtgattact acccaccagc cctgcaaacg aaaattgacg
     5041 aacttaacgg ctggatttat gacaccgtta acaacggcgt gtataaagct ggttttgcca
     5101 ccagccagca agcctacgac gaggcggtgg cgaaagtgtt tgaatcgctg gcgcgactgg
     5161 agcagatttt aggccagcat cgttacctga ccggcaacca gctaaccgaa gccgatattc
     5221 gcctgtggac tacgctggtg cgttttgatc cagtgtatgt gacccacttc aagtgtgata
     5281 agcaccgcat cagcgattac ctgaatctgt atggcttcct gcgcgatatc taccagatgc
     5341 cggggatcgc cgaaactgtc aatttcgatc acatccgtaa tcattacttc cgcagccata
     5401 agaccatcaa ccctacgggg attatttcaa ttggtccgtg gcaggatctc gatgaaccgc
     5461 aggacgagat gttcgcttcg gttaaaaaaa ggcaccttac gaagtgcctc tgataattag
     5521 ctaagtgcat gttgttcata tcggatgcgg cgtgaacgcc ttatcaaacc tacaaaacat
     5581 ttatattcaa tatgttgcaa tgaccgcgca ggccagataa gcgtagcgca tctggcgatt
     5641 ttgcgcttgt catcagtctt agcgcgcctt cacagcactt tattaattcc ttgaacgaat
     5701 atttactgcc atttcattca cgtctattct taatttgctg ctttaaaaac aagtgattga
     5761 gcaaaattga ggcaaaaatg gactggtatc tgaaagtaat aaaaaattat gtcggtttcc
     5821 ggggtcgtgc gcggcgcaaa gagtactgga tgtttattct ggtcaacatc atctttacgt
     5881 tcgtgctggg gctgctggat aaaatgttag gctggcaacg cgctggtggc gaaggcatcc
     5941 tgacgacaat ctacggtatt ctggtgtttt taccgtggtg ggcagttcag ttccgccgcc
     6001 tgcacgacac cgaccgctcg gcgtggtggg cactgctatt cttaatcccg tttatcggct
     6061 ggcttattat catcgtcttt aactgccagg cgggtacgcc aggcgaaaac cgctttggac
     6121 ctgatccaaa actggaacct taaaacaaat ctgctcgccg gatgtctgac tcatccggct
     6181 tttatcatca tgttgatgaa tgaataattt actaatgttt atttaaaata tttcaatgtc
     6241 gttatataaa ttacctataa aaaataacca tgtatttatc ttaaatatca aaacctttat
     6301 aacatccgaa aaagaaagat acgaatagca tttacacaat acccggaata gactttcgtc
     6361 acttttaatt aaagggatgt ttttatgcag tggtatttat ccgtattaaa aaattatgtt
     6421 ggtttctctg gtcgcgcacg tcgtaaagag tactggatgt ttactctgat taacgccatc
     6481 gtcggcgcta ttatcaatgt cattcaattg attttaggtc tggagcttcc atatctgtct
     6541 atgctttatt tgctggcaac cttccttccc gtccttgcgc ttgctatccg t
//
LOCUS       DAZPLI010000197         6553 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZPLI010000197 DAZPLI010000000
VERSION     DAZPLI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.199"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="ACHUU3_003845"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="ACHUU3_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR2083803.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHUU3_003846"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHUU3_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR2083804.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="ACHUU3_003847"
     CDS             2408..3157
                     /locus_tag="ACHUU3_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR2083805.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3745
                     /gene="yieF"
                     /locus_tag="ACHUU3_003848"
                     /gene_synonym="chrR"
     CDS             3179..3745
                     /gene="yieF"
                     /locus_tag="ACHUU3_003848"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFR2083806.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(3799..5136)
                     /gene="adeP"
                     /locus_tag="ACHUU3_003849"
     CDS             complement(3799..5136)
                     /gene="adeP"
                     /locus_tag="ACHUU3_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR2083807.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5302..5967
                     /gene="yieH"
                     /locus_tag="ACHUU3_003850"
     CDS             5302..5967
                     /gene="yieH"
                     /locus_tag="ACHUU3_003850"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR2083808.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6034..6507
                     /locus_tag="ACHUU3_003851"
     CDS             6034..6507
                     /locus_tag="ACHUU3_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFR2083809.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1609 a         1655 c         1658 g         1631 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggctgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagtatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatggaac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactcggtac cgggtgggct gaaaaatgcc atcgactggc tttcccgcct
     3481 gccggatcaa ccgctggccg gtaaaccggt gttgattcag accagctcaa tgggcgtgat
     3541 tggcggcgcg cgctgtcagt atcacctgcg ccagattctg gttttcctcg atgcaatggt
     3601 gatgaacaag ccggaattta tgggcggcgt gattcagaac aaagttgatc ggcaaaccgg
     3661 agaagtgatt gatcagggta cgctggacca cctgaccggg caattgaccg catttggtga
     3721 gtttattcag cgagttaaga tctaaataaa aaacccgcca gtaatcatgc atggcgggtt
     3781 tttaatgcgc tatcgatttt aatgagcgtc gataaataca atcttcagga taaacagcag
     3841 cgcaacgatg attacgcacg ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac
     3901 gcagtaggag ataaagccca gcgcaatacc ttcggtaatc gaaaagctga acggcatcat
     3961 cacggcggta ataaacgccg gaacagattc agtaagatcc tgccagttca cgcgagcaag
     4021 acttgaggtc atcaacacgc caacgtagat caacgcgcca gctgcagcgt agcctggcac
     4081 cattcccgcc agcggcgaca gaaagataac cagcaggaac agcagaccaa caaccactgc
     4141 cgtcagaccg gtacgaccgc caaccgatac gccggaagag gactcaatat aagccgtaac
     4201 ggaagaagta ccgataaacg aaccggtcac ggaagagata ctgtcgacat acagcgcctg
     4261 cttcatgcgc gggaatttcc ccttctcatc cgccagacct gctttatcgg tcacgccaat
     4321 cagcgtaccg gaggagtcaa acaagttgac caacatgaaa gagaaaatca cccctgccag
     4381 cccgaggtta aacgacccgg ctaaatctac atgacccaca actgtcatta cgctcggcgg
     4441 cgcagaaacg atgccattgt agtgcacatc acccagcatc cagcccagca gcgtcgtcac
     4501 cacgatagaa accagcaccg ctgcgtgaat gttgcgcgag gccagaatgg cgatgatgaa
     4561 gaaaccgagg atccccagca gcacgctgtg agaagtcaga ttaccgatgc tcaccagcgt
     4621 ttccgggtta gcgacaatca cacctgcgtt tttcagcccc atcatgccaa tgaacagacc
     4681 gataccgctg gtaataccca cacgcagact taccggaatg ttagcaatca tccagtaacg
     4741 aacgcggaaa atggtcaaaa gaagcagtcc gacagcgccc cagaagattg cgcccatacc
     4801 gatctgccac ggcagcccca ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc
     4861 catcgcagga gccagcgcca ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa
     4921 agcagcaatc agacaggttg ttacgaagac ggcactggtg tccatgccag caacgcccag
     4981 aatttgtggg ttaacaaata cgatgtagac catcgtcagg aaggtggtga acccggcaat
     5041 cacttcagtt cgtgccgtag taccatgttc gcgcagttta aaaactcgtt ccagcatccc
     5101 ctgaccagaa gtctgggtgg tatgttgatg actcattatc tatttccgaa caaggaggga
     5161 aaatccgtcg ctatcgtata ccaaaatgcg acaataggcg cgtttgtgag agactttttt
     5221 tattggattt acttatacgg caacgattgc gttgcgcaaa tcggctctac gaaaacgtta
     5281 aactgattaa aaaggaaagg catgtcccgg atagaagcgg tatttttcga ctgcgacggt
     5341 acgctggtcg acagtgaagt catttgctcc cgcgcatatg taacgatgtt tcaggaattt
     5401 ggtattacgc tcgatcctga agaggtattc aaacgtttca aaggtgtaaa actgtacgaa
     5461 attatcgata ttgtttccct tgaacatggt gttacgttag cgaaaacaga atctgaacac
     5521 gtttaccgtg cagaagtcgc tcggctgttc gattcagaac tggaagccat cgaaggggct
     5581 gaagcgctcc tgtcagcgat cactgcgcca atgtgtgtgg tatctaacgg cccaaataac
     5641 aaaatgcagc attctatggg caagctgaat atgttgcact acttcccgga taaactgttc
     5701 agcggctacg atattcagcg ctggaagcca gacccggcgt taatgttcca tgcggcaaaa
     5761 gcgatgaatg taaatgtaga aaactgcatt ctggttgatg actcagttgc cggtgcgcaa
     5821 tctggtatcg acgcaggtat ggaagtgttc tacttctgcg ccgacccgca caataagccg
     5881 atcgttcacc cgaaagtcac cacctttacc catctttcgc agttaccgga actgtggaaa
     5941 gcgcgtggtt gggatattac ggcatagttc ttcacactcc cttcacttac cccgcttaaa
     6001 ttggcgctca aaggtaagta aagggagttt gatatgtctg tttcacgtcg ggtaatacat
     6061 cacggacttt attttgcggt tttaggaccg ttaattggtg ttctgtttct tgtcctctac
     6121 atattcttcg caaaagaacc gctggttctt ttggtaataa tacaggtact tccattgttt
     6181 ctcttattgt cgattactac gggagctatt cctgcattgt taaccggcgt gatggtcgcc
     6241 tgtctgccgg aaaagatcgg gtcacagaaa aattatcgtt gtctggctgg tggcataggt
     6301 ggcgtcgtta tcaccgagat ctattgtgca gttattgtac atattaaggg catggcttcc
     6361 tcggagttgt ttgaaaacat tctttctggt gacagtctcg ttgtccgcat cattcctgca
     6421 ttgctggcag gtgtggtgat gagcagaatc attacccgtc tgcccggatt ggatatttca
     6481 tgtcctgaaa cagactcttt aagttaagcg ggatacttta tctttgggct actcaaaagc
     6541 agacaggatg tta
//
LOCUS       DAZPLI010000198         6514 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZPLI010000198 DAZPLI010000000
VERSION     DAZPLI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.200"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(300..1283)
                     /gene="zntB"
                     /locus_tag="ACHUU3_003852"
     CDS             complement(300..1283)
                     /gene="zntB"
                     /locus_tag="ACHUU3_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR2083810.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(1297..1344)
                     /gene="ydaN"
                     /locus_tag="ACHUU3_003853"
     CDS             complement(1297..1344)
                     /gene="ydaN"
                     /locus_tag="ACHUU3_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR2083811.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            1538..2770
                     /gene="dgcM"
                     /locus_tag="ACHUU3_003854"
     CDS             1538..2770
                     /gene="dgcM"
                     /locus_tag="ACHUU3_003854"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFR2083812.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(2791..3354)
                     /gene="smrA"
                     /locus_tag="ACHUU3_003855"
     CDS             complement(2791..3354)
                     /gene="smrA"
                     /locus_tag="ACHUU3_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFR2083813.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(3684..4592)
                     /locus_tag="ACHUU3_003856"
     CDS             complement(3684..4592)
                     /locus_tag="ACHUU3_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR2083814.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            4768..6078
                     /gene="abgA"
                     /locus_tag="ACHUU3_003857"
     CDS             4768..6078
                     /gene="abgA"
                     /locus_tag="ACHUU3_003857"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFR2083815.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            6078..>6514
                     /gene="abgB"
                     /locus_tag="ACHUU3_003858"
     CDS             6078..>6514
                     /gene="abgB"
                     /locus_tag="ACHUU3_003858"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFR2083816.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPG"
BASE COUNT         1657 a         1657 c         1592 g         1608 t
ORIGIN      
        1 tttgcaaata aataagtgtc gtcatatttc cctccggtca ttgggtggac tcttaaaggg
       61 tagacgctga taaataacag gcaaaaaaaa gccgactcat caaagtcggc gtcgtacgaa
      121 tcaattgtgc tatgcagtaa ttcaaaaaag gaagtaagac aatatggagc gcaacgccca
      181 tcgcttgacg ttgcattcac ctgcaaggga gatattgccc tgaatgggta gagagtttat
      241 tgacttcgct caaactttgc ggcgtttttg tatacagaca gccggaaaaa ttgcttttgt
      301 tacaaccatt tactacggtg caaccataaa gcaacaccac caataagaac aactaacaga
      361 atacaaaaaa ttgaaaatcc gaattgccac ccgccgccag ggatcccacc aaggttgacg
      421 ccaaataacc cggtcagaaa ggtactgggt aaaaagacca ttgccatcaa cgacattgta
      481 taggtacgac gagctaaatt ttcctgcatc acctgagcga tttcatccgc catcacgcca
      541 gtccgtgcta tacaggcgtc gatttcgtca aggccgcgcc caaggcgatc ggcaatatcc
      601 tgcatccgac ggcgttggtc atcgctcatc cacggcaaac gttcactggc aagacgagca
      661 taaacatcac gttgcggtgc catatagcga cgcatcacaa ttaattgttt gcgcagcaga
      721 gccaggaatc cacgcggtgg aatttgctga tcaaggagat tatcttcaag gtcgataatt
      781 ttatcgtgca gctgctcgat aaattcgctg gaatgatcgg tcaacgcatc gcacacatcc
      841 accagccacc ctccgcaatc ggtcggaccc gtgccctctt ccagatcgct caccacatcg
      901 tccagcgcca gcactttgcg ttgtcgggtc gaaacaatta accgcccgtc catatataca
      961 cgcatggcga ccagttgatc ggggcgttca tcggtgctgc cgtttataca gcgcaatgta
     1021 atcagcgtgc cttcaccgag acggctgact cggggccgcg tgctctcgcc cgccagcgca
     1081 tcacgtacgt tattgggaag cagcggtgtt gtcgccagcc attgggcgct atcatggtgt
     1141 acataattaa ggtggagcca gcagggatgc gcttcatcaa tcacatctgt attttccagc
     1201 ggtttaacgc cgcctctacc atccagcatc caggcaaata ctgcatccgg gacattaacg
     1261 tccgatccct taatcgcttc cacagtgcct ccatcatcaa cgcattattt tgtagtctag
     1321 ccttctggcc ctgttacgca acatctcatc accccattac cctgaaatga ttaataaaat
     1381 tctgtctaaa ttgaatacaa aaagcaaaat gcttttccgt atacaaaccg tgtgaagtgt
     1441 taaatagcgt ctatcattat cagaattatc tgatcatatg acgtggcttt tttgcgatcg
     1501 gatagcaaca aaaattgata aaaataacgg gatctcaatg attacgcaca acttcaatac
     1561 cctggactta ctcaccagtc ctgtctggat cgtttcgccc tttgaggaac agttaattta
     1621 tgccaatagc gcggcgcgac tgttgatgca agacctcacg tttagtcagc tacgaaccgg
     1681 tccctattcc gtctcctcac aaaaagaact gccgaaatac ctctccgatc tgcaaaacca
     1741 acacgatatt atcgaaatcc tcactgttca gcgtaaagaa gaggaaacag cattaaactg
     1801 tcggcttgtt ttgcgaaagc tgacagaagc agaaccggtg attattttcg aaggtatcga
     1861 agcgtcggca acgctgggtt taaaagccag tcgctcggca aattatcagc gcaaaaaaca
     1921 aggtttttat gcgcgctttt ttctgactaa ctctgcacca atgttgttga ttgacccctc
     1981 acgagatggc caaatcgtcg atgctaacct cgccgcgctc aatttctatg gttataacca
     2041 tgaaacgatg tgccagaaac atacctggga aataaatatg ctcgggcgtc gcgtcatgcc
     2101 tatcatgcat gaaatttcgc atttacccgg tggtcataaa cctctgaatt ttattcataa
     2161 gctggcggat ggttcgactc gtcatgtgca gacctacgcc gggccgattg aaatttatgg
     2221 cgacaagctc atgttatgta ttgtacatga tattactgag caaaaacggc tggaggagca
     2281 gctggaacat gctgcccacc atgacgcgat gaccggatta ctgaatcggc gacagtttta
     2341 tcacattacc gagccaggcc aaatgcagca tctcgccatc gcccaggatt acagcttgtt
     2401 gctcatcgac accgatcgtt ttaaacacat taacgatctc tatgggcatt ctaaaggtga
     2461 tgaggtgtta tgcgccctcg cccgcaccct cgaaagttgc gctcgcaaag gcgatttggt
     2521 gtttcgttgg ggaggcgaag agtttgtctt attgctacca agaaccccac tggataccgc
     2581 gctttcgctg gctgaaacta tccgcgtaag cgtggcaaaa gtgagtattt cgggcttacc
     2641 acgctttacc gtcagcattg gtgtggcgca tcacgaagga aatgaaagca tcgatgaact
     2701 gtttaaacgc gttgatgatg ctttgtatcg ggcgaaaaat gatggacgca accgcgtgct
     2761 ggcggcataa gccgcggatg cgtctcgaga tcaacgactg cgcttagcgt ggcgctccca
     2821 gttttcttgc ttcgcctgcg ccgttttacg tagtgcgacg taacacgccc cgctgccgcc
     2881 atgatgcggt agcgcagtgc aatatgcctg aacatcatca aattcggtca gccagcgcgc
     2941 cacatagctg cggacaatat tggcatgcga tttatcatcc cgtcctttgc catgaataat
     3001 cagcacgtta cgcaaaccat cagccatggc ttgttgaatg aaactgaaca tcattttgcg
     3061 gcactcttcc accggctggc gcaaaagatt caggctcgcc tgttgcggat atttaccact
     3121 gcgcagctta tccagcaccc catgttgcaa cccttcccgc cgaaactcca gcggctgact
     3181 tagtgggatg atgtcgagaa atccggtggt gaggaaatta tcaagctgca gcgtgtcgat
     3241 acgctgcggc gcacgttggt tacgcgttgg atgccagtgg acatcggtag cacgtttcag
     3301 cggctggaca tcttccatgg cgtcaagaaa cagcgatttg tcgtcaaggt tcatgttaca
     3361 tcctcccgca attaagagcg cgatatgata accagaccgg gtcggtccaa caacgtatta
     3421 cccaaaattc cagtaataag ttccaaatat tgccgatatt ttaagcaaaa tgcttatgca
     3481 tgattattca ttcacgatat taataatgta acttatattt tcgtgaaatc tgtcactgaa
     3541 gaaaattggc aactaaaggt taaaaccgtt ataacacagt caccggcgca gaggagacaa
     3601 tgccggattt aagacgcgga tgcactgctg tgtgtactgt agagtctggc ggatgtcgac
     3661 agactctatt tttttatgca gttttaactt tgcagatagc cgcattctcg ccggaattgc
     3721 gtgattaatg acgcggtcag tggtgtctgg cgactatcac gccgctgaat caaataatag
     3781 gccgctttcg gtaaaatttc gctaaccggc aacatcacca gcccctgtcc gtgcaaggga
     3841 tcgcagccca tttcttcagg cagtatgctg agaaaatcgc tttttgccac cagactgata
     3901 caggctgaga acgtctcgca gactacaccg acctgtggcg tttgcgcctg atcgtcaagc
     3961 aattcactca actgtttgta gtaactgccg tgtggcgtcg gcattgtcca gctgtaatcc
     4021 agtaactgtt tgatcgaacg ggcaccaatg gcggggtgtc ccgggcggca aaagatcgcg
     4081 aattgctttt ccagtaattt ctcaaaagta aattcgtggt cgtacggtcc ctgataatag
     4141 gtattgatgg tgaaatccaa ttctccctga cgcaattcat taatcatcga caccagttgc
     4201 ccttccataa tgcgtacttt tacctgcgga tgctgctgat gaaaacgaga tatgacagct
     4261 ggcatcagac tgcgggaaat actggccccc atgccgatat taatctgccc tgccagttgc
     4321 ccttgtcgtt ggcgaatatc ctcttgggct gcgcgcagct cttcaagaat aagactggcg
     4381 tgctgataaa aactttcacc ggcatcagtt aacgtcacgc ctttactacg gcgaaaaaag
     4441 agttgcgccg ctaatccttc ttccagctcc tgaatagatt tactcagtgc cggttgcgac
     4501 atattcaaca ttcggctcgc tccgcgaatg ctgccctgac gagccacttc aacaaaagcc
     4561 cgaatttgat gaatttttac ctgaaaagcc atgacgccac cgataaccgt tatttatcag
     4621 accaaagaaa ctggcatcta ctttaatgca gatgattgtg tcagggtaat ttatgaacgg
     4681 ttaaaactgt gaaaaatcag ttagtgataa gtaaaaacta tcgctacgtg aaccgggtca
     4741 cactttttac tgatgacggg aaaggttatg gagtctttga atcaatttgt taattcgctt
     4801 gccccaaaat tatcgcactg gcgacgtgat tttcatcact atgcagagtc tggctgggtg
     4861 gaattccgca ctgccaccct tgttgcggaa gaattgcagc agctcggcta ttcactggcg
     4921 ctgggccgcg aagtagttaa tgaaagtagc cggatgggac tacctgatga actcactcta
     4981 caacgcgaat tcgagcgcgc tcgtcaacag ggtgcgctag cacaatggat tgcggttttt
     5041 gaaggtggtt tcactggtat cgtcgccacc ctggataccg gtcgccccgg tccggtgatg
     5101 gctttccgtg tcgatatgga cgcgctggat ctcagtgaag agcaggatgt cagccatcgc
     5161 ccctaccgcg acggttttgc gtcatgtaac gccggaatga tgcatgcctg tggtcatgat
     5221 ggacataccg ccattgggct tgggctggcg cataccctta aacaattcga gtccggacta
     5281 catggcgtca tcaaactgat ttttcagcct gcagaggaag gtacgcgtgg cgcgcgggcg
     5341 atggtcgatg caggtgtcgt agatgatgtt gattatttta ctgccgtgca cattggcact
     5401 ggcgtacctg cgggcaccgt ggtgtgcggc agtgataatt ttatggcaac caccaaattt
     5461 gacgcgcact tcaccggtac cgccgctcac gcaggcgcaa aaccagaaga cggtcacaat
     5521 gccttgttgg cggcagcaca agccactctt gcactgcatg caatcgcccc gcacagcgaa
     5581 ggagcttcca gagtaaacgt gggcgttatg caggcaggaa gcggtcgtaa cgttgttcct
     5641 gcctcggcgt tgctgaaagt ggaaacacgc ggggccagcg acgtcattaa tcaatatgtt
     5701 tttgaccgtg cacaacaagc gattcagggc gcagcaacca tgtatggtgt cggcgttgaa
     5761 actcgtctga tgggtgcagc taccgccagt tctccttcgc cgcaatgggt cgcatggttg
     5821 caaagtcagg cggctcaggt cgcgggggtc aatcaggcca ttgaacgtgt tgaagcgcct
     5881 gcgggttccg aagatgccac attaatgatg gcccgcgtgc agcaacatca agggcaagcc
     5941 tcctacgtgg tgtttggcac acagctggcg gcaggtcatc acaacgaaaa attcgatttt
     6001 gacgagcagg ttctcgctat tgccgtcgaa acgctggcgc gcaccgcgct caattttccc
     6061 tggacgcgag gtatctgatg caggaaatct atcgttttat cgacgatgcg attgaagccg
     6121 atcgccaacg ttataccgat attgccgatc aaatctggga tcatccagaa acacgttttg
     6181 aagagttctg gtcagcggag catctggctt cggctctgga atctgcaggc ttcaccgtta
     6241 cccgcaacgt aggcaatatc ccaaatgcct ttattgcttc gtttggtcaa ggcaaaccgg
     6301 ttatcgccct gctgggagaa tatgacgccc tggcaggttt aagtcagcaa gctggttgcg
     6361 cgcaacctac atccgcgacg cccggtgaaa atggtcacgg ttgcggacac aatttgcggg
     6421 gaaccgccgc ctttgccgct gcaatagccg tcaagaaatg gctggaacaa tatgggcaag
     6481 gcggcacagt gcgcttttat ggttgtcctg gcga
//
LOCUS       DAZPLI010000199         6401 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZPLI010000199 DAZPLI010000000
VERSION     DAZPLI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.201"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..330
                     /locus_tag="ACHUU3_003859"
     CDS             <1..330
                     /locus_tag="ACHUU3_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR2083817.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIV
                     RDVMTWGDADNQQVKLSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNS
                     VRNFIVE"
     gene            complement(531..1388)
                     /gene="sitD"
                     /locus_tag="ACHUU3_003860"
     CDS             complement(531..1388)
                     /gene="sitD"
                     /locus_tag="ACHUU3_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR2083818.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1385..2242)
                     /gene="sitC"
                     /locus_tag="ACHUU3_003861"
     CDS             complement(1385..2242)
                     /gene="sitC"
                     /locus_tag="ACHUU3_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR2083819.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGSLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2239..3066)
                     /gene="sitB"
                     /locus_tag="ACHUU3_003862"
     CDS             complement(2239..3066)
                     /gene="sitB"
                     /locus_tag="ACHUU3_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR2083820.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3066..3980)
                     /gene="sitA"
                     /locus_tag="ACHUU3_003863"
     CDS             complement(3066..3980)
                     /gene="sitA"
                     /locus_tag="ACHUU3_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR2083821.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4566..5000)
                     /gene="uspF"
                     /locus_tag="ACHUU3_003864"
     CDS             complement(4566..5000)
                     /gene="uspF"
                     /locus_tag="ACHUU3_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR2083822.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(5141..6274)
                     /gene="ompN"
                     /locus_tag="ACHUU3_003865"
     CDS             complement(5141..6274)
                     /gene="ompN"
                     /locus_tag="ACHUU3_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFR2083823.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT         1739 a         1512 c         1547 g         1603 t
ORIGIN      
        1 attgaagcat ggcgcacaga acaggaggcg aagccgttca cgtttgaatg gaacggtcgt
       61 atctggaatg ctggccccaa ctcactgggc cgcctttatc cggtggtaat ggctgcgaaa
      121 tccgacattg tacgggacgt gatgacgtgg ggtgatgccg ataatcagca ggtgaaactg
      181 tcgatgccgg aactggaaga actggcggca gcaatggctc aggcacaggt tgagcgcaat
      241 gacgagattt atcgccgtca gcgggagctg aaggaagagc tgaatagtct gaaggatttg
      301 aattcggtta ggaattttat cgtggaataa cagaagctgc ggcacgtcgt atgcaggaac
      361 gtgccgcggt tggctggtaa actttcgata gtgcgagtat tgaatgattt ctaggcgtta
      421 tcgattttac gtattttttg catgatagaa ttcgtacctc ctcccaacga ccttccatgg
      481 ctttccggac ttctgtaggc tggcggacga tgctgtcctc ggatgttgtg ctaattgggt
      541 gataacagat cctgtgtatg ggcgtttccc tttgtgcgtg cgttgatgct ggtaacggta
      601 aacgtcatga tgaacacgac cgcgaataag acgacaatcg tcggagctgg tgcgctgtcg
      661 ataaaaaagg acagccagac accagtcatt gacactatta ccgatatgcc agtcgccagt
      721 aggagcgcaa tatgaaagcg ttgtgtcagg agtactgcaa tggcgccagg cgcaatgagc
      781 agagaaatag agagaatgat acctactgct ttcagcgtcg ccacaatggt cagggaaacc
      841 atgcacagca gcccataatg cagccatctc gtatgcaaac cgcttacctg cgcctgttga
      901 taatcaaagc tgaacagcaa aaaatcccgc cattttgcgc taataacaag tgtaaccagc
      961 ccggcaataa tcaccgtctg gattatatcg ccgatggtta tcccgagcat gtcgccgaaa
     1021 agaatatggt caagatgtac gtctggtttg attgcaatat acaagatcag acctgcggca
     1081 aacatgccag aaaaaacaat ccccataact gtatcttgct tgattcggct gttgtctttc
     1141 aggtatcccg tcgccaccgc gcagaatact ccggcgacga atgcccctgt agccagaggt
     1201 aaccccagaa tccaggtaag cacgatgcca ggaaaaaccg catgactcat cgcgtcaccc
     1261 atcagtgccc aacctttcag gaccagaaag accgacagca gcgcacaggg tatggaaact
     1321 accagtgaaa tcagcagtgc gtgactcata aaggtgaact gcagtggctc cagtaataat
     1381 gccatcatga gcgatcctcc agcgctttat gcgcacgccg acgatttgcc aaaagtccat
     1441 gggtgggcgc aaatacaaat gctaaaagaa acagcagtgt ctgcgccacc acgatgattc
     1501 cacctgtcgc gccgtccaga aagtaactga cccacgcgcc gaggaaactg gttacactgc
     1561 caatagttac agcaattatg agcaggcggg gaaaacgatc ggttaacaac catgctgttg
     1621 caccgggagt caccaccaga cagatcacca gaaaggcacc aaccgtttgc agcgccgcaa
     1681 cggtcgaaac cgacagcagg gtgaagaaga ggatttttaa tctgcccgga tgtaatccaa
     1741 tagcacgggc gtgattctcg tcgaaaaagg tcaccataag atctttccat ttgaaaaaaa
     1801 gcacgatgat cgagagaatg ccaatgatag tgagttgcag gatatccgcc ggatcgattg
     1861 ccagaatatt gcccaggacg atggtctgaa tgttcacgga tgtcgggttc aatgacacca
     1921 taaacagccc caggccaaaa aaggacgaga agatcaggcc gatgatggca tcttctttta
     1981 agcgggtgcg ttggttaaga aacagcatgc tgcccgctgc cagtccgccg gaaaagaagg
     2041 ctcccagaga aaacggaagc cccagcatat atgcccccgc aacacccggg acaatagagt
     2101 gcgacagcgc atcgccaatc agcgaccagc cttttagcat cagatagcat gacaggaaag
     2161 cgcagagact gccgaccatt gccgagaccc acatcgcatt gagcatatat tcatagctga
     2221 agggttccag tagcacgttc atcttgcttc cctctgcacc gctgacggtc gatgtgccac
     2281 aaaagggcgt tcatcgtcag taataatgct ttcttccgag tcgttaagtg tgacatggcg
     2341 cagtacgccg ctaaaagcca gctccaggtt ctcggctgta aaagtggtgt ctgtcggccc
     2401 gcttgccagg acggtgcctt tgaccatgac cgtataatcg caaaatgtcg tgactgagcc
     2461 gagattgtgc gttgagacaa gcatcgtttt gccttccgcg cgtaactccc tcaacaggct
     2521 gatgattttc gcttcggttt tcacatcaac gccggtaaaa ggttcatcca gcaggatcac
     2581 atcaccctgc tgtgcaatcg ctctcgccag aaagacgcgt ttcttttgtc caccggaaag
     2641 ctcgccgatt tgacgatggc gaaaatccac catatcgaca cgttccagcg catcggtgac
     2701 aatctgacga tccctctttt tggctatacg caacataccc atatgcccat agcggcccat
     2761 catcaccaca tcttccacca gtacaggaaa tgaccagtca acctcttctg actgagggac
     2821 gtaggcaacc aggtttttct gtagcgcctg tcgcgtggga atacccagaa cagatatctt
     2881 tccgctggtc agacgcacaa atcccataat cgctttaaac aacgtcgatt ttccggaacc
     2941 gtttacccca accagagcgg caatagatcc acttggtact gtgaaggatg catcgcgcag
     3001 tgctgtgtgc ccgttacgcc aggtgacagt aacatcatta acgacaatgc ctgcagattg
     3061 catcattatt tctccctttt cccggcttta attccctgta ccagcgtact ggtagtaacc
     3121 ttaagaaggt cgatatacgt aggtaccggg ccgttttctg tgctcaggga atcgacatag
     3181 agcacaccgc cgtagtgtgc gccggtttca cgcgcaacct gacgcgctgg tttatcggaa
     3241 atcgtactct cgctaaagac tgccgggata tgatttttct taactatatc aacaacctta
     3301 cgtacctgct gcggtgttcc ttgttgatcg gcattaatcg gccacagata aagctctttt
     3361 agccccaaat cgcgtgcgag ataagaaaaa gccccttcac tggtgaccat ccatcgctga
     3421 ttctcaggga gttccgtaat ctgcttacgc aggggggcaa gggtttgggt aatcttggct
     3481 ttataagtat cggcattacg ttggtaggtt tgtgcatttg ccggatcgta ttttatcaac
     3541 gcatcacgaa tattatcgac gtaaatcaga gcattatctg gcgacatcca tgcatgggga
     3601 ttaggtttgc cctcataggg tccttcggtg atccctactg gcgtcacacc cgaagagaca
     3661 attacttctg gaaccccatt gagatgctgg taaaagcgtt ggaaccacaa ttccagattc
     3721 ataccattgg cgagaatcag ttgtgccccc tgcgcacgtt taatatcgcc aggggtaggc
     3781 tgatactcat gaatttctgc accaggcttg gttatggatg agacttctgc agcatctcca
     3841 gccacgtttt tggccatatc cgcgatgatg gtaaatgttg taatgacctt gaatttttca
     3901 gttgcacttg cctgaaatgc gatcgagcag gtgagggcga ggcaccccaa gagcatggtt
     3961 acttttttta ttgagtgcat aatttagtac ctatagtgag caaaattaga acaaacatag
     4021 caacggctat gttttatagc ataacccata caaaatgaaa atgattatca ttaacagctt
     4081 tgaggcctaa aacagggatt aggttgtttc ctgctggcga catgatattg aggaaaaaag
     4141 tagagcagct attaggcttt gaaatgactt tatgaggaaa attgttgatg gcaaaaatgg
     4201 ggcaaaacgc tgtagaaggg gcaaaaaagg ggcaaaaaaa gagtgggtta tcgtagctta
     4261 ttgttgtcgc tgataatgtt taacatattg aaaaataaat aaaatactta tgggtcagat
     4321 agttgtgatt tttgccctta cttgttcagg ttgtattgtt ctttcttact aatttcttga
     4381 ttttgcgaca tttaaaagcg actcaattcg ttatatggca tcagaagagt atgcgtcatg
     4441 ccggaacgcc cagcataaga aatctgatat aaaactgtgg cgtgtatggt acggattaga
     4501 ggggaaaatg tcagcacatt tgcgaaatga atcaaaaagc ccgcagcaat gtgcgggcgt
     4561 tagtgtcagc gcacaaccag cacggagcac tctgcgtgac gcactacagc tgcggcgttg
     4621 gaaccgagca gataagtggt gatatccggt cgatgggaag caatgatgat catatgagcg
     4681 gggatcttct tcgccaattc cagaatgcgg tctttgggcg agccttcctc aacatggaca
     4741 tgcactctgt cggttggcag tttaaatttt ttaatgatct cttccagttg cgatttggct
     4801 tccgctttca ggtcatccat tgccggtaat tctgcggaat acgctaaacc cagagaggca
     4861 tagtaaggca gtgaaggtat taccgtcagg aaatgaacct ctgcatcgtc aatctttgcc
     4921 tctgcctcaa cgtggctaat cacgcgttga gttaattctg aatcggaaat atcgataggg
     4981 acaagaatcg ttctgttcat aaaacctcct gttttagtat ccgcataaag tgtaacgcca
     5041 gatgacactt tttgtgtaat gacggagttc acatttttaa tttagatcaa aggaggaaga
     5101 ataagcagaa aaagcccgcc ataacagcgg gcaggaggat ttagaactga taaaccagac
     5161 ctaaagcgac aatatcatcg gtagagatgc cgttggcagc gtagaaactg tcatcttcat
     5221 ccaacaggtt gattttatag tcaacataag tggacatgtt tttattgaaa tagtaagtcg
     5281 cgccaacatc ggcgtattta acaagatctt tatcatcaac acctgccggg ttgtctgcac
     5341 catcggcagc gtgcaggtca cggcctttag acatcaggaa agagactgcc ggacgcagac
     5401 caaaatcaaa ctggtactgt gcagtgactt caaagttctg ggttttgttt gcaacagcat
     5461 aatcgctgtc gccaaacggg gtcatattac gcgtttctga atacatggtt gccaggtaaa
     5521 tattgttagc atcgtatttt agcccagcag tccacgcgtc tgctttatca ccacccgccg
     5581 cagtatggtt aacctggtca ttggtgcggt cagatgaggt gtatgccgca ccagcgctaa
     5641 agcccatgcc taaatcatat gttgtggaaa gaccccagcc gtcaccattt tcatggcgaa
     5701 catcacgtcc gttgttggtg ccttcctgac cattactggc accttcgttg ttaccttgat
     5761 actgcaccgc gaagttcaga ccatttacca gaccgaagaa atcagtatta cgataagtcg
     5821 cgacgccgtt ggcacgacca gtcataaagt tgtctgcatt ggtataagag tcaccgccaa
     5881 attcaggcag catatcggtc cagccttcga tgtcgtacat tacgccataa ttacgtccgt
     5941 aatcgaaaga accgtaatct gcaaatttca gcccggcaaa tgccagacgg gtccatgact
     6001 ggttttttga agattcagtg ttgtttgcct gaatattgta ttcccattga ccgtagccag
     6061 tgagttgatc gttaatttgg gtttcgcctt taaaacccag acgcgcatag ctctgatcgc
     6121 catctttcgc tgaattatca gaaaaataat gcaggccatc aactttgcca tacagatcta
     6181 atttgttgcc gtctttatta taaacttcgg ctgcatgtgc agcacctgcg gcgagcaggg
     6241 caggaattaa aagtgccagt actttgcttt tcattgaata aatcctttag ttattttatt
     6301 tgccttttat ccccattccg gggagtgaaa acatcctaaa ggagaagttc agttagccga
     6361 taaatatcat ttgttacgcc ttaagtaaaa ccttaatcaa a
//
LOCUS       DAZPLI010000200         6375 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZPLI010000200 DAZPLI010000000
VERSION     DAZPLI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.202"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            190..2037
                     /gene="ldtD"
                     /locus_tag="ACHUU3_003866"
     CDS             190..2037
                     /gene="ldtD"
                     /locus_tag="ACHUU3_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR2083824.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2218..2766
                     /locus_tag="ACHUU3_003867"
     CDS             2218..2766
                     /locus_tag="ACHUU3_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR2083825.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2793..3440
                     /gene="gloC"
                     /locus_tag="ACHUU3_003868"
     CDS             2793..3440
                     /gene="gloC"
                     /locus_tag="ACHUU3_003868"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR2083826.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3662..4852)
                     /gene="aspC"
                     /locus_tag="ACHUU3_003869"
     CDS             complement(3662..4852)
                     /gene="aspC"
                     /locus_tag="ACHUU3_003869"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR2083827.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5037..6125)
                     /gene="ompF"
                     /locus_tag="ACHUU3_003870"
     CDS             complement(5037..6125)
                     /gene="ompF"
                     /locus_tag="ACHUU3_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR2083828.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1606 a         1564 c         1613 g         1592 t
ORIGIN      
        1 tacggcgcgc ttttctttaa acttctttac attaggttat gtaaaaacgt atcggcgttt
       61 atatactgaa gataagcctg atgagtaaca ggcttgctcg tcatactttc gtgagtattg
      121 gcgttgtaca ggcaagtcgt aaaataacag cctggctatt cagagtatga taaaaacagg
      181 gggcaaggga tgttgcttaa tatgatgtgt ggtcgtcggc tgtcggcaat cagtttgtgc
      241 ctggccgtaa cattcgctcc actgttcaat gcgcaggccg atgagcctga agtaatccct
      301 ggcgacagcc cggtggctgt cagtgaacag ggcgaggcac tgccgcaggc gcaagccacg
      361 gcaataatgg cggggatcca gccattgcct gaaggtgcgg cagaaaaagc ccgcacgcaa
      421 atcgaatctc aattacccgc aggttacaag ccggtttatc ttaaccagct tcaactgttg
      481 tatgccgcac gcgatatgca acccatgtgg gaaaaccgtg atgctgttaa agccttccag
      541 caacagctgg cagaggtggc gattgccgtt ttccagccgc agtttaataa atgggtagag
      601 ttactgaccg atcctggtgt taacgggatg gcacgcgacg tagtgctctc tgatgcgatg
      661 atgggctatc tccatttcat tgcaaatatt ccggtcaaag gcactcgctg gctatatagc
      721 agtaaacctt atgcacttgc aacgccgccg ctctcggtga ttaaccaatg gcagctggcg
      781 ctggataaag gtcaattgcc tacgtttgtt gcaggactgg caccgcagca tccgcaatat
      841 gcggcgatgc atgaatcgtt actggcctta ctcagtgaca ccaaaccgtg gccccaactg
      901 accggcaaag caacgttgcg cccagggcag tggagtaacg acgtaccggc gttgcgcgaa
      961 atattgcaac gcacaggcat gttggacggg gggccgaaaa ttactctacc tggcgatgac
     1021 acgccaactg acgcggtagt cagcccatcc gctgttactg ttgaaacagc agaaactaag
     1081 ccgatggaca agcaaacgac gtctcgtagt aaacctgcgc ctgccgttcg cgccgcctac
     1141 gataatgaac tggtggaagc cgttaaacgt tttcaggcat ggcaaggatt gggggcagat
     1201 ggtgctattg gcccggcaac acgtgactgg ttaaacgtaa cgcccgccca gcgtgctggc
     1261 gtgttggctc tcaacatcca gcgattgcgc ttgctgccaa cagagctttc taccgggatc
     1321 atggttaaca ttccggccta ttcgctggtc tactatcaga acggcaatca ggtgctggat
     1381 tcgcgagtca ttgtcggtcg ccccgatcgc aaaacgccga tgatgagcag tgcccttaac
     1441 aacgtagtgg taaacccgcc gtggaacgta cctccaactc tggcacgcaa agatattctg
     1501 ccaaaagtgc gcaacgatcc gggatatctc gaaagccatg gctatacggt gatgcgcggc
     1561 tggaacagca gagaagcgat tgacccatgg caggttgact ggtctacaat cacggcctcg
     1621 aatttaccgt tccgcttcca gcaggctcca ggcccacgga actcgctggg gcgctataaa
     1681 ttcaatatgc cgagttcaga ggccatttat ttgcatgaca cgccgaacca caatctgttc
     1741 aagcgtgata cacgcgcatt gagctcaggc tgtgtacgag tgaataaagc ttccgatctg
     1801 gcgaatatgc tgttgcagga tgcaggctgg aatgacaaac gtatttctga tgcgctgaag
     1861 cagggtgata cacgttacgt caatattcgg cagtcgattc cggtgaatct ctactacctg
     1921 acggcctttg ttggtgcaga tggtcgtacc cagtatcgta cagatattta caattatgat
     1981 ctgcctgcgc gatccagctc gcaaatcgta tcgaaagcgg aacaattaat caggtaaatg
     2041 aagtagttcg gggaaatcag ttgtcgtaat aattagcatg attgggggcg attctctgca
     2101 gcccccgtca ctgctggggt tgagtcatct tgacgtctgc tttacgggcg gttaaggtgc
     2161 ctcttgtgcg ccagaagtgc atataaacga taacattgac ctgtagactt gattatcatg
     2221 gacaaattcg acgctaatcg ccgcaaattg ctggcgcttg gtggcgttgc actcggtgcc
     2281 gccatcctgc cgacccctgc gtttgcaaca ctctctaccc cacgcccgcg cattttgaca
     2341 ctcaataatc ttcataccgg agagtcaatc aaagcggagt ttttcgatgg cagaggctat
     2401 attcaggaag aattggcaaa acttaaccat tttttccgcg attaccgcgc gaacaaaata
     2461 aagtccatcg acccaggatt attcgaccag ttgtatcgcc tgcaagggtt gttaggcacg
     2521 cgcaaaccgg tgcaactcat ttccggttat cgttctattg ataccaacaa tgaactacgc
     2581 gcccgcagcc gtggagtagc gaagaaaagc tatcacacta aaggccaggc gatggatttc
     2641 catattgaag gtatcgcgtt aagcaatatt cgcaaagccg cgttatctat gcgcgcaggt
     2701 ggtgtaggat attatccacg tagtaacttt gtgcatattg ataccgggcc agcacggcac
     2761 tggtagtaat tgcttaacga aacaggggca gtatgaacta tcgtattatt ccggtcaccg
     2821 cattctccca gaactgttca ttaatctggt gtgaacaaac ccgtctggcc gcactggtcg
     2881 atcctggcgg cgatgcggaa aaaatcaaac aggaagttga tgacagcggc ctgacactga
     2941 tgcagatcct gctgacgcat ggtcatctgg accacgttgg cgcagcggcg gaactggcgc
     3001 aacattacgg cgtgccggtt tttggcccgg aaaaagaaga tgagttctgg ctgcaaggct
     3061 tgcctgcgca aagtcgtatg tttggtctgg aagagtgcca gccgctgacg ccagatcgtt
     3121 ggctgaacga aggcgatacc atcagcatag ggaatgtgac tttacaggtg ttacattgcc
     3181 ctgggcatac gccgggtcat gtcgtgtttt ttgatgatcg ggcaaagctg ctgatttctg
     3241 gcgatgttat tttcaaaggc ggagtagggc gcagtgactt cccgcgtggc gatcataatc
     3301 aactgatttc ttcaatcaaa gataaattgc tgccactggg ggatgacgtg acatttattc
     3361 cgggtcacgg accattatcc acacttggtt atgaacgcct gcataatccc ttcctgcaag
     3421 acgaaatgcc cgtctggtaa ggcacataaa aaagcccgct tttaatgcgg gcctggattt
     3481 ctggcaaagt gcgctttgtt tatgccggat gcggcacgag cgccttatcc ggcctacaaa
     3541 atcgtgcaaa ttcaaaatat tgcaggggac gcgtaggcct gataagcgta gcgcatcagg
     3601 caatgttgcg tttgtcatca gtctcagccc gcttttcagc gggcttcatt gtttttaatg
     3661 cttacagcac tgccacaatc gcttcgcaca gcggagccat gttatctggt gtcatcccgg
     3721 ccacatttac gcgaccagaa gcaaccgcat atacgccaaa ctcttcgcgc agacgcagca
     3781 cttgttcttt tgtcaggcca ctgaaggaga acatgccgtt ctgtttgatg ataaagctga
     3841 agtcgcggtt tgcgcctttt tcctgcagcg tattgacgaa caactgacgc atacgctgaa
     3901 tacgctggcg catatcagtc agctcttgtt cccaaatcgc acgtaacgca tcgttgctca
     3961 ggatggtggc aacaacagaa gcgccgtgtg ctggtgggtt agagtagtta gcgcgaatcg
     4021 ccgctttcat ttggctgaat gcgcgatcaa cggtttcact gtcggcagca accagagtac
     4081 aagcgccaac acgctcgttg tacaggccaa agtttttaga gtaggaactg gcaacaatca
     4141 gctctttatg catagccgcg aaagcgcgca gtccttcagc atcttcttcc agaccacggg
     4201 caaaaccctg gtaagcgaag tcaaacagcg gtaaccagcc tttctcaacg gagagttgtg
     4261 ccagtgtctg ccattgttcc agcgtagggt cgataccggt tgggttatgg cagcagccat
     4321 ggaacagcac tacgtcgcca gcctgagctt cattcaggct gttaatcagt gcatcgaagt
     4381 caagagtgtg attttccgca tcataataag cgtattcacg aacttccaga cctgcagagt
     4441 taaagacgct cttatggttc ggccagcttg ggttgctcac ccacactcgc ttaacgctgg
     4501 tattttttgc caggaaatcg gcagctacgc gtagtgcgcc agtgcccccc ggagtctgtg
     4561 ccgtgcgagc acgtttgtca ttgatcaggg cgctaccttt accaaacagc agttcctgag
     4621 tgcagcgacc aaattcaggg atgccgtcaa tgccgaggta atttttggtg gtttcatttt
     4681 cgagcagata ctgttcagcc tttttcacgc tggtcagtac cggggttttg cccgtctcat
     4741 ctttatagac accaatcccg aggttaattt tgccgggacg ttcatcggca cgaaacagat
     4801 cggccaggcc cagaatcggg tcggcaggag cggcggtaat gttctcaaac atgacgaggt
     4861 tccattatgg ttacagaagg gaagtccgct atcagggtaa cgggagattt acaaaattcc
     4921 aactattact gatgaaaacg caggctgttt ttgcaagacg tgagattgct ctggaaggta
     4981 taaaaaaaac aggaccaaag tcctgttttt tcggcattta acaaagaggt gtgctattag
     5041 aactggtaaa cgatacccac agcaacggtg tcgtctgaac ctacgcccag tttgttgtca
     5101 gaatcgatct ggttgatgat gtagtcaaca taggtggaca tgtttttgtt gaagtagtag
     5161 gttgcgccca cttcaaagta gttcaccagc tcaacatcac cgataccttc tacgtctttc
     5221 gctttagatt tggtgtaagc gatggacgga cgcagaccga aatcgaactg gtattgcgca
     5281 actaacagaa cgtcttgcgt tttgttggcg aagccgctgg tgtttgtaaa tttattagtg
     5341 atcggcgtag cgttacgggt ttcaccgtag ttcgctgcca ggtagatgtt gttcgcgtcg
     5401 tacttcagac cagtagccca ctgttcagct tttttaccgt tgccaagagg ttgagcttct
     5461 tgcaggttgg tacggtcagc tgcaccataa gcaccaacga taccaaagcc ttcgtattcg
     5521 tagctgatag aaccgccaac accgtcgccg ttagaacggc gtgcagtgtc acgctcgttt
     5581 ttacccaggt actgaacagc gaagttcagg ccatcaacca gaccaaagaa gttggagtta
     5641 cgataggtag caacgccgcc aacacgacca acgaagaagt catcgctgta tgcagtatca
     5701 ccaccaaatt ctggcagcat atcggtgtaa cccagtgcat cataaaccac accgtagtta
     5761 cggccgtaat cgaaagaacc aacgtcagcg tatttaagac ccgcgaatgc cagacgcgtt
     5821 ttgttaccag tttgagcgtc agcgccttca gagttgttac cctggaagtt atattcccac
     5881 tgaccataac cggtcagatc ggaattgatt tgagtttccc ctttaaaacc aagacgggca
     5941 taggtcatgt cgccattgcc accgtaactg ttttcaccgt tacccttgga aaaataatgc
     6001 agaccaacag ctttaccgta cagatctact ttgttgccat ctttgttata gatttctgca
     6061 gcgtttgcag tacctgctac taacagagca gggacgatca ctgccagaat attgcgcttc
     6121 atcattattt attaccctca tggttttttt tatgacacct gccactgcgt caataagttc
     6181 tgtcaataaa aatttacgga actattgatg agagtttggt gtctttatgt gtctgcaggc
     6241 atctttccat tcaaactaac gtttcgctac cgtgaaagtg ctacaaagat aaagatttag
     6301 tttcaaaaag aaaaaatatg taaccaaaag taaaaattaa ggaactttgt gaacaccgtc
     6361 atatttccat agaaa
//
LOCUS       DAZPLI010000201         6319 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZPLI010000201 DAZPLI010000000
VERSION     DAZPLI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.203"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            171..1769
                     /gene="rtcR"
                     /locus_tag="ACHUU3_003871"
                     /pseudo
     CDS             171..1769
                     /gene="rtcR"
                     /locus_tag="ACHUU3_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1751..2509)
                     /locus_tag="ACHUU3_003872"
     CDS             complement(1751..2509)
                     /locus_tag="ACHUU3_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR2083829.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHUU3_003873"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHUU3_003873"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR2083830.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHUU3_003874"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHUU3_003874"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR2083831.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="ACHUU3_003875"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="ACHUU3_003875"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR2083832.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="ACHUU3_003876"
     CDS             complement(5474..6079)
                     /locus_tag="ACHUU3_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083833.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..>6319)
                     /locus_tag="ACHUU3_003877"
     CDS             complement(6082..>6319)
                     /locus_tag="ACHUU3_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083834.1"
                     /translation="CEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIENPLK
                     QPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
BASE COUNT         1577 a         1652 c         1623 g         1467 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgccagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagcacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaa
//
LOCUS       DAZPLI010000202         6302 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZPLI010000202 DAZPLI010000000
VERSION     DAZPLI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.204"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..273)
                     /locus_tag="ACHUU3_003878"
     CDS             complement(<1..273)
                     /locus_tag="ACHUU3_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083835.1"
                     /translation="MTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNAHILE
                     HGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIV"
     gene            complement(332..4189)
                     /gene="sigA"
                     /locus_tag="ACHUU3_003879"
     CDS             complement(332..4189)
                     /gene="sigA"
                     /locus_tag="ACHUU3_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR2083836.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4551..5555)
                     /locus_tag="ACHUU3_003880"
                     /pseudo
     CDS             complement(4551..5555)
                     /locus_tag="ACHUU3_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5540..5917
                     /locus_tag="ACHUU3_003881"
                     /pseudo
     CDS             5540..5917
                     /locus_tag="ACHUU3_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5956..>6302)
                     /locus_tag="ACHUU3_003882"
     CDS             complement(5956..>6302)
                     /locus_tag="ACHUU3_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR2083837.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         1571 a         1408 c         1410 g         1913 t
ORIGIN      
        1 gacaatgctg tattcttttt taaagcactc accagaaacg gtgattgtaa tgataatggt
       61 cttctcgatc gagatgtaaa tacccgtttc gttatactgt ccgtatcgga accatgagaa
      121 ctcagttgca ttccagccat gctccagaat atgtgcattt ttttgcctga tagtttcttt
      181 ttttattcgc tcatgtttaa ggcataacag gcagataaat aatgtcagaa aaagaaagct
      241 aataatgaga gcaagagcga tatagtctgt cacagggtaa tacatattct ctacggtaga
      301 agaagggccg caaacgcggc ccgggctgtt atcagaaaga gtaacggaag ttggcgttga
      361 tcgcgttatc cacgttgtat ttaccaaatg ccgatttctc aaactcaaga ccgaagcgaa
      421 gattatcgcg aatttcggcg ttgagaccaa cattcatgag aatacgaccg tctttttcac
      481 ctttgatacg tttctcaccg gacgcatcac gcagtacggt ttcaccgttg gcaaacaggt
      541 caaactggta gccaaggccg gcgcgggctg tgactttcca gtccttaccg gagaaggatt
      601 tacccacatc aacaccggta cgcccaatca gcggattaaa gtccttatcc ttcatggtca
      661 cgctcattcc gcgatcctgc cagtcgaacc gtttacctga tacggcccca taaaccagtt
      721 ctgcctgcgg ctcaatccag gagtcttctg ttacatgata gcggtagcct gcttcagcac
      781 ccacatacaa ggaatgagaa ctgtagtctt ttgttccgag tccagcaaag gtcgccgagt
      841 actcattatc atggtgaaca tacttactaa tcaggtcgat ataggcacca gagtcaaata
      901 tagcggaagc atacagacct gccccgacgg acttcgtctt gccactgaat gcattactgc
      961 tggcatgact gtcggtatac gtcatagtca gaccagtgaa aaggtcaagt ccatccagct
     1021 catgtttttt atccacacca atctgcacat gggtgtagtt gtcactgtat ccactagaag
     1081 ctgaacctgc tccgctcatg atgcgggccc atgcaccggc ctcgccattg gtgtcacgca
     1141 gatcccccat tcgtttattc aggttgttga cttctgtcag gaaggctttg taattaccgg
     1201 acatatacga ttttgctgca ccggaagatg ctgtattttc agccacctta tatccggtca
     1261 gggtccacgt ggtatgttcc tgatcctctt tcgtgctgat cacaggagat atgttgctga
     1321 aacctatggc ctgagtactg gctttaaata tctctttatt gcttcctttt ggggctgtta
     1381 tcaggtcaac gtttaatcct gagttattat ttcccacttt ctgtatatcg accgacagac
     1441 tgttattgct tccggttaac tgatttttaa caatgagttt atccgattga tttacagact
     1501 ggctcagatc agcccgcata atcacagcag tattagtggc atccagtgtg tttactgtca
     1561 cagtatggaa acccgaattc tctatgttac taaacaacaa aagactgtcg cgggttttta
     1621 atgattgcag ggctgactgt ccgtttactc tccataaggt gttatacatg ctggctgagg
     1681 cagcaggagc atcaatacct ccggtataag aagtatcaaa gccacccagc aaacctgtag
     1741 ccagcggtgt gtgtgtggtt ttgtccaggt tatcttcttt atactggtca acaccaaaca
     1801 gaatagatgc ttttttgtct gattttatat taccattgac ccaggcataa ttttgagcct
     1861 ttaacgtggc accatcttct gtcatattat atccatctga catatagatt gccgggctgt
     1921 atttattatt ttctgtaggg gtgcctgtta atgtcagggt tccggccagt tcaatagctt
     1981 tatctacata catttttccc tgggcggtaa cagtagctcc tttgttgata atcaggtctg
     2041 tattaattaa ggataatgca ccagtcatag taacagttga tttatcacca acagttattt
     2101 tgctgttttg agcactcaga ctgccgttga aactactttc gcctgctgaa tccccggagc
     2161 gaagctgttg tctgaatcca aaaccttcgc cagtaatgtt tcttcctgag aacatgtcaa
     2221 tgtatgcagt tttatttccc agaacaattt gagagttttt agcctctatg tgtcctttta
     2281 cattagcatc ccggcctata ctcagggttg cgttttctaa atttaatttc cggaaattaa
     2341 atttacgact ttcccagtcg ggctgggaaa aagaggctat ctgattgttg gacttatatt
     2401 ccgtattgta acgtttattt acagtgcttt cctggtcctt tatggcagca gaatagtctt
     2461 tttgacataa aaaaggaatt ggacagttgt tattgccttc tttaaatatg gcatgttctg
     2521 tcgcatgtcc ttgtaatgtt aactgtgcat tccggacaga gatatcgttt ttgatatcga
     2581 ctgagccatc aaagatcaga ttggcattat tgttatgttg ctgggtagta ttgataatat
     2641 gatttatgct tatattacct gatacattcc catggtacat atatgcatca gtattagtaa
     2701 gtgataatgt tgattgcttc acgtttgagt tcgttacggt tgttcccgca tctgttgctg
     2761 cgattttctg aaatgactgg tcatagccgt ttagatccag tgttccgcca tttctggtaa
     2821 aatagatttc gccattaccg ctttcgctta aagcgtcttt ggcatttatt tttaccgtac
     2881 ctttacctcc ggccatgtaa attttattga aggctttttc agcactaagg atgacagtcc
     2941 cattacctat tttaaggtta tttccctgtg ctatttttac atccagagta ccactaccta
     3001 ttttatgcag attatctccg gcaacgccct taacattcca ttcaacggtg gtattattat
     3061 caatatctat cccggcacct ttataagact tatcttttcc gtacacttta tatgtctggt
     3121 tcttatcgaa tataagcccg ccaatgccaa gatcggtatc ctgcttgagc gttaattccc
     3181 ctcctcctga gacagacagg tccttatctt ttataatcga tgataactct tgttttttac
     3241 catccagctg catagtgttt gtgaaattct tttttaaatt gtttaccaga atattattaa
     3301 ataacgagta gtttgtccag gctatattgg tactactgag aaaaccaact gtactggtaa
     3361 caccatggaa aacccatttt tgttgttctt tatcatatag ataagaacca gaaccactat
     3421 cgccaaaaat tggtgagttt ttaaaagggg ttctgttggt ataaatgtaa ctcttgtttt
     3481 tccagtccag attaaaaaga cttgcgctta acaagtcggg attatagttt tgtccaaaca
     3541 gatattgttt gccgtttaat atcgttgagg ttgttccggc acctgctctg aagccaatta
     3601 tttgttcctt accgttgtag tttacgccat atcttttcat cgcatcttcg ggagaaagtg
     3661 agaagtcggc actctccgta acccctgttg tttccacgac gaatttattc agacgatgaa
     3721 ccgaaaagtc gccagatgat actctgttac ttgctttata tgctgtctga tcccatgact
     3781 gtgttgttat tgcatgatgc tgtgtaccgt tatgagtcgc agtaacactg tacgcacctc
     3841 caattgatgt tgttgcacct ttgttggaaa cagcagaaaa atcaggtaca ggtagttctg
     3901 gaaaatgaaa tatctttcca tctttcatca ttaattgaac atttgttgca cccggtctga
     3961 actctcctct gttttgagca aggtcaagat aatcgcgggt ccatatatta tttatatcca
     4021 gcattgctgc aaatgctgtt tcgccataac ttccatatat tgaggataaa ataataccga
     4081 ggataacttt ctttcttgat gtaccgacac taactttccg ggtcagttca gaaacagcaa
     4141 ctaatccacc tgtaatatga ctatatttca gtgaataaat tttattcata cgtgaactcc
     4201 gatatttctg agttgtggtt atgggatagc atttaattac aacggggaac tatcagtcat
     4261 ggtacgtaac tgataagggt ataatgataa tcactgtcag ttatatttgt atgacagatg
     4321 taacaaaatg aaaaaaaaag ataagcacaa gtgattgatt ggtcagatat tgatcacttg
     4381 gtgtttgtat tttgaacttt gcgatcaaaa tttgatcttt gttgtttccg gtcaatcctt
     4441 tacaggtttt ctgtattatc cgggggagtt aagtctgtcg atgtccgaca gacttttgat
     4501 gatggaaaca acatgcagcg ataatgtgca tgctgttttg caggtggaac tcagaaggtg
     4561 aactggaact gtgcgaccgc accgtaagtg ttgtccgtgc cgatcaggcc tgcaacatca
     4621 aggtgaacca catcaaacgg tgagatcccc accccggccg tgaatgcact gccgtcgtta
     4681 ccggccagat tctgacggta accggcgcgc agctgtgccc atttccaggc attaaattct
     4741 gcgccaatgg cggcaaactg acggttgctg tcggaggtga aaccgctggc cggggtcaga
     4801 tccacatcaa atgcggtggt gaacatcgcg ttgtgccagg agacaccggc cgtcacctgc
     4861 gggcggatcc tgaaggtttc cgtgataccg ttcacttctt ttgtctcaat actacggggg
     4921 atcaggttct gtgcgaccag tcccagcgtc cagttgtcat ccaggtccgt actggcaccg
     4981 atgtcggcgt tgatgccatt tttcgtgttg tggtagcggt cgcctttgaa ggcgctgctg
     5041 tcataatttc tgaccagcac gttgtagttg aacaggtcga cgcgctggaa tttggggggg
     5101 aagcccagag accatttctg cccggccgtt tccagttccg tagccagagc aatacccaca
     5161 tcagtgatca gtgccgcgcg ggcaaaggcc tgtgatgtca ggcggctttt gtccacctcc
     5221 tgcccggtgc tgtttgccac ctcttccagg taagtgaggt cagcatcaga gactttaccg
     5281 tcgacactga cggtgccgtg tgatttaacc atcagtgcgg cagagaggcg atcaccggga
     5341 agggccgcca cagcagaaac gcccagctgt gcggcggcat gtgtgttacg gaagtcccgg
     5401 aggtgttctt tcaggcgtgc ggcagcctca ggaacaccgt ggttactttc gatggcgcgg
     5461 tcgaaggcct tccactcatc actgatccgg tcggcgttgt cggtgatatt atccggattg
     5521 gagagctgcg cgccgacgga cggaagcagg atgctgggct catgcgcgac gcaaaatcca
     5581 cgatgtatat atcagtacta aaagcgcgac ggcggaagaa gctctgaaac taatcggtga
     5641 actgtacgcc atagagcacg atatacgcgg gttaccggtg tctgaacgcc tggcggtcag
     5701 gcaaatgcag agtagaccgc tcctgacttc cctgtataag ctgatgcagg agaaagaaca
     5761 cacgttatcg aagaaatgtc gtctgggaga tgcgttccgg tatatcagga agcactgggc
     5821 tgcgttgtgc aacttctgtg atgatggcct ggctgaggta gataataatg cagcagaaag
     5881 agcgcttcgt gcagtctgtc tcgggaagaa aactttttcg aggatcactc attccgtgtc
     5941 tgactgcttc agggtttaat tttgcttctg ctgactgaat gatgattggg cagcaagtgc
     6001 aatatcccac ataaatccag ttaactcacg agccaccgcc gtaatggcta cttgtgattt
     6061 tttccctttt gctgtcagag ttcggtaacg agaacacaat cgttgttgag ctttccatgc
     6121 aatatctttg gaatattgtg ttacagagtc cggtcggtaa tatataagcc atgctccaac
     6181 ttttgcaggt gtacgataag accaagcggc ttcgtaaagc agacgtctca gttcgctgtt
     6241 tccaactttt gttattcctc tgggacgaat actgtttcca ctggaatatt cacctggcac
     6301 ca
//
LOCUS       DAZPLI010000203         6209 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZPLI010000203 DAZPLI010000000
VERSION     DAZPLI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6209
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.205"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            45..1505
                     /locus_tag="ACHUU3_003883"
     CDS             45..1505
                     /locus_tag="ACHUU3_003883"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR2083838.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1541..1744)
                     /gene="ydfZ"
                     /locus_tag="ACHUU3_003884"
     CDS             complement(1541..1744)
                     /gene="ydfZ"
                     /locus_tag="ACHUU3_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR2083839.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1921..2607)
                     /gene="rspR"
                     /locus_tag="ACHUU3_003885"
                     /gene_synonym="ydfH"
     CDS             complement(1921..2607)
                     /gene="rspR"
                     /locus_tag="ACHUU3_003885"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR2083840.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2696..3442)
                     /gene="ydfG"
                     /locus_tag="ACHUU3_003886"
     CDS             complement(2696..3442)
                     /gene="ydfG"
                     /locus_tag="ACHUU3_003886"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR2083841.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3579..5624
                     /gene="dcp"
                     /locus_tag="ACHUU3_003887"
     CDS             3579..5624
                     /gene="dcp"
                     /locus_tag="ACHUU3_003887"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR2083842.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5669..6187)
                     /locus_tag="ACHUU3_003888"
     CDS             complement(5669..6187)
                     /locus_tag="ACHUU3_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR2083843.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1547 a         1485 c         1596 g         1581 t
ORIGIN      
        1 taacaaaaca tatagcttgc catatttata tttaaggaat tgttatggga aataatttgt
       61 tatcagcaaa agcgacactc cctgtttatg atcgtaataa cctggcccca agaattattc
      121 atttaggctt tggtgcattt caccgtgcgc atcagggcgt gtatgccgat attcttgcta
      181 cagaacattt cagtgactgg ggatattatg aggtcaactt aatcggcggc gaacagcaaa
      241 ttgccgattt acaacagcaa gataatcttt ataccgttgc ggaaatgtcg gccgatgcgt
      301 ggacggctcg cgtcgttggc gtcgttaaga aagccttgca cgtacagatg gatggcttag
      361 aaaccgtgtt ggctgcgatg tgtgaaccgc aaatcgcgat tgtctctctg acaatcaccg
      421 aaaaagggta tttccactct ccggcgaccg gacagttaat gctcgatcac ccgatggtcg
      481 ctgccgacgt gcaaaatccc caccagccga aaacagcaac aggggtgatt gttgaggcgc
      541 tggcccgccg taaagcggca ggacttcccg catttaccgt catgtcatgt gacaacatgc
      601 cagaaaacgg tcatgttatg cgtgacgttg tcacttcata cgcgaaagct gttgatgtaa
      661 aactggcaca atggatcgaa gataacgtga ctttcccatc aacaatggtg gaccgtattg
      721 tgcccgcagt gacagaggat acgctggcga aaatcgaaca acttaccggt gtgggcgatc
      781 ctgcgggcgt tgcctgtgaa cctttccgcc agtgggtaat agaagataac tttgttgccg
      841 gacgtccaga atgggaaaaa gcgggagccg aactggttag tgatgtgctg ccttatgaag
      901 agatgaagtt gcgcatgctc aacggcagtc attcattcct ggcgtatttg ggttatcttg
      961 ctggatatca gcacattaat gactgcatgg aagatgaaca ttatcgttat gcggcgtatg
     1021 gcttgatgtt gcaggaacaa gcgccgacgc tgaaagtgca gggcgttgat ttgcaagatt
     1081 acgctaaccg attaattgaa cgctatagca acccggcgtt acgtcatcga acctggcaga
     1141 ttgcgatgga tggcagccag aaattgccac agcggatgtt ggattctgtt cgctggcatc
     1201 tggcgcatga cagcaagttc gatctgctgg cactgggcgt cgcgggttgg atgcgttatg
     1261 tcggtggtgt tgatgaacag ggaaatccga ttgaaatcag tgacccactg ttacctgtta
     1321 ttcagaaggc tgtacaaagt agtgctgaag ggaaagcgcg cgtccagtca ttgctggcga
     1381 ttaaggcgat ctttggtgat gatttgccag acaatagttt gtttactgca aaagtgacgg
     1441 aagcgtactt gtctttatta gcgcatggcg cgaaagcgac cgtggcgaaa tattccgtga
     1501 agtaaaaaaa gcaatgccgc catcggcggc attcacacgc ttagttcatg ccgagacgaa
     1561 tcaggtccag aggtgccagt ttctcttcac aaccttcaac aactacggtt tttccgcggc
     1621 ggatttgctc agcggtcaga ccttcagtat caatcgacag gatcttgcca gtgtgaccgg
     1681 tgccgctaac cataacacgg ctgccagtgg tgattgcgtt acggttacga tcgtaggtcg
     1741 tcataggtat tttctccttt ctgatttaca gtcgaagccc acgtaacggg cgcaatataa
     1801 atactcctgt cgagtgaaat attttttgtt tttgatcaag ctcacatctt ttttgataca
     1861 tgcatgagcc ataaggcggg ggaaaagact ctcaccccgg atgggatgag aggaaatgaa
     1921 ttactcttcg ctaaaccagt cgctattttc ctggcggatc tggcgcacgg attcgctgat
     1981 ttcctgcaaa tgttgcgtca ttgcacgttc taccgcatcg ccatcacgtt tttgcagggc
     2041 agagaaaata tcaagatgct ggcgtaacag catttctggt ggagaaacgt ggtcgaaact
     2101 catatagcgc acgcgatcaa cggtcgcttt caggttctca atggtatccc acgccagttg
     2161 acagtcggca atctgcgtca ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc
     2221 cagttgcttg cgctcaatgg caatgcgttg ctggtgaaga ttttgttcca gttggtagca
     2281 ctggctttcg gtaatcatgc tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg
     2341 gataaaactg ccattgcgca cctgggccat ggaaattttg ttgacgtagc tgccacgctg
     2401 cggacgaatt tgaatcaggc cgttttccgc cagtttaata aaggcttcac gaaccggctg
     2461 gcgtgacaca ttgaaacgaa cagaaacttc tttttccgac aacggtgtgc ctggtgcaat
     2521 caggcaatgg acaatgtcgc gacgaagaat acgataaatt tgctgattga caggctgtgt
     2581 gggattaagt tgcgtttcga cggtcattcg ttcttgcttg tgagtgagtt aactgcatga
     2641 gtctaccact tttttgtgac aagcaataac ccggcagtta cgccgggtat aaaaattact
     2701 gacggtggac attcagtccg gcatagcttt gggtaaccgg catcatttcc agggtattga
     2761 tattgacgtg agcaggcagc gttgacaccc accagacggc ttcgctgaca tcttctggcg
     2821 tcaatgcaac ggtattttga taggtttttt ccgctttacc gtcatcgcct ttaaagcgga
     2881 cattggaaaa ctcggtgcca cccaccagac ccggttcgat gtcggtgacg cgcaccgccg
     2941 taccatgcag gtccgtacgc agattcaggc taaactgacg aacaaacgct ttcgtcgcac
     3001 cgtaaacgtt accaccggca tacggccagc tacctgccgt tgagccaatg ttaataatat
     3061 gaccatgatt acgttcaacc atacccggta agacggcgcg cgtcatatat accaggcctt
     3121 tgttgttggt atcaatcatc gtttcccagt cttcaacgct ggctttatgc gcaggctcca
     3181 tgcccaacgc caggccggca ttatttacca ggatatcaat attgcaccac tcggcaggaa
     3241 gcgatgccag catctcttca atagcggcgc ggttgcgaac gtccagttgg gcgatataca
     3301 gattatctcc cagttcgtct tttaactcct gcaaccgctc ctggcgacgg ccagtggcga
     3361 taactttatg cccttgttga ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg
     3421 ctccagttac taaaacgatc atctcactgt tcctcaacgc ttttgtgtgt caataacata
     3481 gcacgtgatg cttaagagcg ggtaatactg gtttgcaaga ttgcactccg ttatcagtaa
     3541 gcctacgcta tggaacatct cgcgttcagg agaagaaaat gacaacaatg aatcctttcc
     3601 ttgtgcaaag cacactaccg tatctggctc cccattttga tcaaattgcc aatcatcact
     3661 atcgcccggc attcgatgag ggaatgcagc aaaagcgggc agaaattgct gccatcgcgc
     3721 ttaacccgca aacgcctgat ttcaacaata ctattctggc actggaacaa agcggagaat
     3781 tacttacccg cgttaccagc gtcttttttg cgatgactgc ggcgcatacc aatgatgaat
     3841 tacagcgtct tgatgaacag ttttccgctg aactggcgga actggctaat gatatctatc
     3901 tgaacggtga attattcgcg cgggtagatg ctgtctggca gcgccgtgaa tccctggggc
     3961 ttgatagtga atccatccgc ctggtggagg tgattcatca acgttttgtc cttgccggag
     4021 ccaaacttgc gcaagctgat aaagcaaaat taaaagtact gaatacagaa gctgcgaccc
     4081 tgaccagcca gtttaaccag cgattactgg cagcaaataa atccggcggt ctggttgtga
     4141 acgatatcgc gcagctggca ggaatgagtg agcaagagat tgcgctggcg gcagaggcgg
     4201 ctcgcgagaa aggtctggat aacaaatggc tgattccgct gctgaatacc acccaacaac
     4261 cggcgcttgc cgaaatgcgc gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga
     4321 cgcgagcgga aaaaaatgat ggcaatgata cccgcgctat cattcaacgt ctggtagaga
     4381 ttcgcgcaca gcaggcgaaa ctgcttggtt ttcctcatta tgccgcatgg aaaatcgccg
     4441 atcagatggc aaaaacgcca gaagcagcac tcaactttat gcgggaaatt gttccagcgg
     4501 cgcgtcaacg tgcgagcgat gaattagcct ccatacaggc ggttatcgat aagcagcagg
     4561 gcgggtttag cgcgcagccg tgggactggg cattttatgc ggaacaggta cggcgggaga
     4621 aatttgatct tgatgaagcg cagctcaagc catattttga attaaacacg gtgttgaatg
     4681 aaggtgtatt ctggaccgcg aatcagctct tcggtattaa gtttgtcgaa cgttttgata
     4741 ttcctgtcta ccatcctgac gttcgggtgt gggaaatttt tgatcataat ggcgtggggc
     4801 tggcgttatt ttacggtgat ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg
     4861 gcaattttgt tgagcaatca acgcttaatg aaacgcatcc ggtaatttat aacgtctgca
     4921 attatcagaa acccgctgcc ggtgagcctg cgttgttact ctgggatgat gtaataacct
     4981 tattccatga atttggtcat acgctgcacg gcctttttgc ccgccagcgt tatgccacgc
     5041 tttccggcac caacacgccg cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact
     5101 gggcaacgca tccgcaggta ttcgctcgct acgcccggca ttatcagagc ggggcagcaa
     5161 tgcctgacga actgcaacag aaaatgcgta atgccagcct gttcaacaaa gggtatgaga
     5221 tgagcgaact gcttagcgcc gcacttctcg atatgcgctg gcattgcctg gaagaaaacg
     5281 aagcaatgca ggatgtcgat gattttgaat tgcgggcgct ggtggcggaa aatatggatc
     5341 ttcctgctat accgccacgc tatcgcagca gttatttcgc ccatattttt ggtggcggat
     5401 atgctgcagg ttattacgct tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt
     5461 ggtttgttga gcagggcgga ttaacgcgtg aaaatgggca gcgttttcgc gaggcgatcc
     5521 tttccagagg taacagcgaa gatctggaac gcctgcatcg acaatggcgc ggtaaggctc
     5581 ctcagattat gccgatgctg caacatcgtg gcttgaacat ataaacacca ccaatggctg
     5641 gcctggggat atcgccaggc cagcctggtt atagcagtaa ttgcagcagc tgggcgaggg
     5701 caaacctcac cgctaaagct aataccgttt cgcagtcgcc agcaaaatgc ataaccgcag
     5761 tgtagttctg gcctttaata tgccacgcga accagacggt acccgctggc gtaccatctt
     5821 caccgccctc cggtccgccg tagccggtaa tggcaatact gacatcagca tccgcacgct
     5881 ctatggcacc tgttgccatt tctgctgcca ctttctcact caccgcagaa tatcgttcaa
     5941 gagactgctg gcttacgctg aggattttca tctttgcctg atcggtgaaa gtaacaaagc
     6001 ctgcaccgta aaatttgggt gtatcttcag ctgcacacag ggcgctagcc aacttaccgc
     6061 cggtgcatga ttcggcggta gttaagcgta attgatccgc gataagtctt tgactcaacg
     6121 cggatgtcag gttttcaata gtgtcggtat ctgcgagttg tacaatttta tctttattaa
     6181 tgttcactgt tttttgtcca cgctattat
//
LOCUS       DAZPLI010000204         6121 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZPLI010000204 DAZPLI010000000
VERSION     DAZPLI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.206"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..358
                     /locus_tag="ACHUU3_003889"
     CDS             <1..358
                     /locus_tag="ACHUU3_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR2083844.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPA
                     YTSQRCACCGHTAKENRLSQSKFRCQVCGYTVNADVNGARNILAAGHAVLACGEMVQS
                     GRPLKQEPTEMIQATA"
     gene            complement(398..1069)
                     /locus_tag="ACHUU3_003890"
     CDS             complement(398..1069)
                     /locus_tag="ACHUU3_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFR2083845.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="ACHUU3_003891"
     CDS             complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="ACHUU3_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR2083846.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="ACHUU3_003892"
     CDS             complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="ACHUU3_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR2083847.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2798..3211
                     /gene="yedD"
                     /locus_tag="ACHUU3_003893"
     CDS             2798..3211
                     /gene="yedD"
                     /locus_tag="ACHUU3_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR2083848.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="ACHUU3_003894"
     CDS             complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="ACHUU3_003894"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR2083849.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="ACHUU3_003895"
     CDS             complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="ACHUU3_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR2083850.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="ACHUU3_003896"
     CDS             complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="ACHUU3_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR2083851.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5600..>6121)
                     /gene="fliD"
                     /locus_tag="ACHUU3_003897"
     CDS             complement(5600..>6121)
                     /gene="fliD"
                     /locus_tag="ACHUU3_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR2083852.1"
                     /translation="YKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAF
                     KTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKG
                     YLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTS
                     TYLSQQFTAMSNS"
BASE COUNT         1497 a         1609 c         1527 g         1488 t
ORIGIN      
        1 gccggggcgc aatgtccggg caaaatcagg tttaaaccgt tcgatactgg atcagggctg
       61 gtatgaaatg cgccgccagc ttgagtacaa gcagctctgg cgtggcggtc aggtgcttgc
      121 tgttccgcca gcgtacacaa gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa
      181 ccgcctgtca caaagtaaat tcagatgcca ggtatgtgga tatacagtga acgccgatgt
      241 aaatggcgct cgtaacattt tagcggcggg gcacgccgtt cttgcctgtg gagagatggt
      301 gcagtcaggc cgcccgttga agcaggaacc caccgaaatg attcaggcga cagcctgaac
      361 gtagcaggga tcctcgtcct tcagggcgtg gaggatgtca acaaacaggt tgaattaact
      421 ccgcgccctg gtttttgaca ttacctaccg cgcgcgatac cgggtgccag gtgaactggt
      481 ttgctggcac acagccgttg gtagcaattt ctgatgcttc tttaccgcca atctcttgcc
      541 gcatccattc ctgtgcagct tctggcgaca gaaccagcgg gcggcggtca tgaatgtcta
      601 ccagaccctg gtctgctgca gcagtgacaa ttaaaaatcc ttcggcttcg tcaccacgct
      661 caaatggtgt gctacctatc gcggccataa aaataggttg tccatcagca cgatagataa
      721 aaaaaggctg ttttttgtcg ccttcttttt tccactcaaa ccagccatcg gcaaaacaga
      781 ttgcccgacc atgttgccag agcggtttaa acatgcgact ggttgcggca gtttctacgc
      841 gggcgttaat cagcggcggt ttatcccacc atccgggagc atatccccag aaaaccggat
      901 ccagatgaag gtgttcatca cgttcactga gcagaagaac tttggttccc ggcgcgacgt
      961 tgtatctgcc aatgggttcg ggatcgtagg gaatatcgcg ttcaatatct tccgcgagaa
     1021 gcgcaaggta atcttcacgc gtttgggatt gggcaaagcg tccacacata gaaacctcct
     1081 gagatcatac tgaaaagaat aggagagggg gcgggtaaaa tctatgtgtc agaggcaaca
     1141 atgttcccgc tgtgggccac agcgggaatg agggattact tctgaattaa ataacgaatg
     1201 gtcggcccgt cttgctgaat atccagcacc gtatagccgt gattacgcgc atccagtgga
     1261 atattattga tcgactgtgg acagtcgctc accacttcca ggatttcccc tttttttaac
     1321 tgcggcatcg cctcaagggt tgcgactgcc ggataagggc agggttcacc caccatatca
     1381 aggcggtaat caggaacgat atttttcatg cgatctcctt agcagtctgc ggtgccgcac
     1441 ggcggaagaa gcgtttttcc cagccgataa tcaacatcag cgcagtaaac aacagcaaat
     1501 atgtcaccag caggccaccc atcgggccaa aggttttcag caggttgatt ttgtcccagt
     1561 cggtggccag cgccggagcg aaatcatccc agtaatacgc cagaatcgtc gagccgatca
     1621 cattgcccag accgacccac cagtagtgca cctggccttc taccgcgcgg tacatccagc
     1681 cggtttcgca gccgcctgcc agcacgatgc caaaaccaaa cagtaaacca ccaattaccg
     1741 cgtttggtcc cgcccacatg attttgggtt caacgcctaa ctgtacgtaa ctgaagatcc
     1801 cgatggcact caccgccata ccgataatga ttgctttcgc catatgggta cgtccggtga
     1861 tccacatatc gcggaacgcc gaagtaaagc agatttgcgc acgttcaatc agtaaaccaa
     1921 agccgacgcc aaacagcatt gccagcccca gtttgggttg gttcatcgcc gtgagcagcg
     1981 cccagcccag catgccgaaa aagaccagca tcccgagacg aaaacgacgc cgcgcctgat
     2041 ccggtttttg cgtcagcggt gaggcggcag aaactttctg cattttcacg ggaatacgga
     2101 agatgggcag cagggtaaag cgcgcgccaa accatgaacc aatggcagtg gcgatggcaa
     2161 agaaccaggc atgcagcgag aactgaggaa tgccggtaaa gaacgccgcc aggttacagc
     2221 ccattgccag acgcgcgcca aaaccggcga taatgccgcc aataatggcc tgcataatgc
     2281 ggatacggct gcgcggcatg cgcagtttga cattgttggc ccacagcgct gcggcaaagc
     2341 agccgccaaa catgccgagg atcatcatcc cgtcgatgcg ggttaatggc gatccttcca
     2401 gatggataat tttaaaataa ccccactctt cagcatggac gccaaacagc tgcaggagct
     2461 gaccgcccca acgggtaaat tcacccgtga cagcccaaaa ggtgccagta atgccaaaat
     2521 agtaagtgga gagaataccc gccgcgatga ccgcagggat gggtgcccag aatttaatca
     2581 accaggcgtg tttgaattgc tgccatgaca tgaatgtagc ctctgaactc agaaggtttg
     2641 aaacaagaat cgcgaatcat acatcatgca aatttatttt cggggtaata attcacaaaa
     2701 ataatagtta gatcaaattt ctctctgaac ctggaaaggc tggcgacatc agggctttaa
     2761 tgccacaatc ctcttttctt ctcatgcagg acgaatgatg aaaaaactcg caattgcagg
     2821 tgcgctcatg ttgctggcgg gctgtgccga agtcgaaaac tataacaatg tcgtgaaaac
     2881 ccccgcgccg gactggctgg cgggctactg gcagaccaaa gggccgcaac gtgcgctggt
     2941 cagtccggaa gcgataggca gcctgattgt cactaaagag ggcgataccc tggattgccg
     3001 tcagtggcag cgggtgattg ccgtgccggg caagttgaca ttgatgtctg atgatctcac
     3061 taacgttacc gtaaaacgtg agctgtacga ggttgagcgt gacggtaaca ccattgagta
     3121 cgatggtatg acgatggagc gtgtggatcg cccgacggca gagtgtgccg ccgcgctgga
     3181 taaagcgccg ttaccgacgc cactgccgta atgaaatctc cccggcttgc cgccggggat
     3241 gaatttaaat cacctcttcg ataacccaca cgctgacgct gccgccgttg caaaagaagg
     3301 tcgcttcgcc gttttcgtcg gtcactacgc tctcttgccg attccccaga aaatcacgcc
     3361 aggttttatt gccgtaattc tctcccagat gaatggtttt ttcgccatca tccccgttcg
     3421 acatgaccac cacgcagccg ggatattcgt cagtgccact gcggctaaag gcaatgcagt
     3481 tcggatggtc gaaaaataac gtctgtacac cgtgggcgaa acgctgacgg gcgagaatta
     3541 actcatcaag ctgttcgatt attggcatat ctatcggata ggtttgcccg tcaccaccga
     3601 catcttcgta atgcgcaccg tagaggtccg gatagaacac cgaaggaacg ccattttccc
     3661 gcaacaaaat tagagcatac gccagcggtt taaaccacgg ttcgaccggc gcttcgaggg
     3721 cttgcaacgg ttgggtgtcg tgattggcta cgagggttac ggcgtggaaa ggatcggctt
     3781 ccactaatgt acccgtgaaa atctgcgtca tgtcgtagtc gcgtcccatg cgcgatgctt
     3841 catggaattt catctgcagc ggcgcatcga acagcatggt tttgccttcc acctgatcaa
     3901 tatacgtttg cagtttatcg acttcatgcg accagtactc tgccacaata aacagcggct
     3961 ttggcgcaac ttcctgtacg tgttcgatcc actctttata aaaccacgcc ggaatatgtt
     4021 taaccgcatc aagacgaaaa ccgtcgcatt gtgtttgttc catcacccag cgcgcccagt
     4081 atttaatctc ttccgtcacg gcatgattgc ggaaatcgat attctcaccc atcagataat
     4141 cgaaattacc gagttcatca tcaacctgat cgttccagcc ttcgccggtg tagtcgttaa
     4201 caattttaaa tatgccatct tcatcaggat tttcgatatg gtcgataccg ctaaaacatt
     4261 tgaagtccca gataaactgc gagtattgcc cggcacgggc ggggaaggtg taacgcgtcc
     4321 agccttcgca ctcaatgatt tcttcatcaa tttgcgtacg gtcatcagca tttacacgct
     4381 gcacgcgaat agcttctttt tcatccgccc ccattttgtg gttgactacc acgtccagta
     4441 gcaccgcaat gtcattacgt ttcagagcgt caatggcggc cagcagttgt gctttatcgc
     4501 catatttagt agggatgctg cctttctgat caaactcacc taaatcaaat aaatcatagg
     4561 agtcgtagcc gaccgaatac ccgcccgatg cgcctttata ggcgggcggc aaccagacca
     4621 tattgatacc aatatcatta aaaccgtcgg cgcgctcggc cagttcaggc cagagcttac
     4681 cgccttccgg gtaataccag tgaaaacatt gtaacagcgt gggattacgc attcttcgac
     4741 tccattcaag gggaacatta gaagcgtagc cgtaatcgga ttattcgcga gccatcggct
     4801 cgttaagatt caaaagaggt tatcctgcgg agccagcaca aagccgccct gatctccata
     4861 ggcacttaac actgattttt gcaccgatga ctgaccgacc agtttcgcca gttcatccat
     4921 acgaatctgt aataactgct ttaccttgct ttcgttgtcg agaatcaggc gcagcatcgg
     4981 gcggagctgc tcctgcatcg tggtggacgg ggcaacctct tcagtcagat gtgcaatctc
     5041 ctgcaccgca ttcacatacg ccatttcgct ggcgatgagt tcgtcccatt gttcttccgt
     5101 tgccaggcgt aacatgagct ggcttttatc gacgagttgt tgccaggcga aatacaaatg
     5161 cggtgcatgg ttcatcagac tgggtcctga atcaaagaag gggagagtaa agactctttc
     5221 caggcatcgg caatattgcg cattaatgct tccacttctt cgactgcgga gacatcgttg
     5281 cgtaaattgg cttgcagcaa gcgcctgacc atatagctat aaagagcaat caagttttgg
     5341 gttagttcgt ctttgctctc ttcatcaaga ctcacccgca gcccgttctc aatgatgttg
     5401 atcgcttttg acaaagagac gcctttgcct tgctgattgt tgtcctgcat aaacaggcgc
     5461 gctctaacca gtgcgctcag cactccatca aatagcatgg tgaccagctg ttgctggctg
     5521 gcgctcatta cggcgctttc gacgccaatt tgtgcatagg cttttgagcc gctcgcggta
     5581 tacatgctga cctccgtgat caggaattac tcattgcagt aaattgttgg ctgagatagg
     5641 tactggtgtt attcagtttg ctcatcatgg tatctaattg cgtaaactgt gcagtgtaac
     5701 gcgccacggt gtcatcaatg ctggcactga cggatagata ctgtttggtc agttttttca
     5761 gtgtggcgtt aatactgtcc tgtgcgctat caataatgcc atcatcggca aggtaacctt
     5821 ttacctcggt ggcaattttg gtggtaattc ctgtttcttt accatcgcct accagcaatt
     5881 cacgtacgga tgctgtattt tcattcagga cttttttgag tttatcatca tcaattttca
     5941 gcttacccgt cgtgccgtcc tgcttaatcc cgatctcgtt taatgttttg aaggcactgt
     6001 cgctggcacc gtttgcaaat tgtgcgcgga tgccgctttg aattgtacgt acaacgctgt
     6061 ctccgagaag tgcaccatta tttttatctt gtgcttcagc acctgggtct acttctttat
     6121 a
//
LOCUS       DAZPLI010000205         6056 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZPLI010000205 DAZPLI010000000
VERSION     DAZPLI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.207"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(193..384)
                     /locus_tag="ACHUU3_003898"
     CDS             complement(193..384)
                     /locus_tag="ACHUU3_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083853.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(381..803)
                     /locus_tag="ACHUU3_003899"
     CDS             complement(381..803)
                     /locus_tag="ACHUU3_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HFR2083854.1"
                     /translation="MYCTVKEVIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRASRHVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGETMS"
     gene            complement(850..1275)
                     /locus_tag="ACHUU3_003900"
     CDS             complement(850..1275)
                     /locus_tag="ACHUU3_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR2083855.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     KYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            complement(1688..2458)
                     /locus_tag="ACHUU3_003901"
     CDS             complement(1688..2458)
                     /locus_tag="ACHUU3_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083856.1"
                     /translation="MNKTLNALVYRHARNLLLAQGWPEETDIEQLNPHYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEEEIRAVLDAVRDAVCSVSCRVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDYPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSECVEHILSSGLVCDVLR
                     IPDEPSRRWFDRDILREVVLEARDEIRSMVDALAKIRG"
     gene            complement(2503..3022)
                     /locus_tag="ACHUU3_003902"
                     /pseudo
     CDS             complement(2503..3022)
                     /locus_tag="ACHUU3_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(2952..3173)
                     /locus_tag="ACHUU3_003903"
     CDS             complement(2952..3173)
                     /locus_tag="ACHUU3_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083857.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            complement(3174..3857)
                     /locus_tag="ACHUU3_003904"
     CDS             complement(3174..3857)
                     /locus_tag="ACHUU3_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR2083858.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            complement(3934..4227)
                     /locus_tag="ACHUU3_003905"
     CDS             complement(3934..4227)
                     /locus_tag="ACHUU3_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083859.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDG
                     RFYRSRLALVTVYASREPFSDERPSSRFRGIALPSERRRLRYSTVGLTRYRTR"
     gene            4375..5337
                     /gene="parM"
                     /locus_tag="ACHUU3_003906"
                     /gene_synonym="stbA"
     CDS             4375..5337
                     /gene="parM"
                     /locus_tag="ACHUU3_003906"
                     /gene_synonym="stbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR2083860.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKIILNGGDTFTIKDVKVMPESIPAGYEALQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            5340..5690
                     /locus_tag="ACHUU3_003907"
     CDS             5340..5690
                     /locus_tag="ACHUU3_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR2083861.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRAPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            5949..6056
                     /locus_tag="ACHUU3_003908"
                     /pseudo
     CDS             5949..6056
                     /locus_tag="ACHUU3_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         1558 a         1670 c         1504 g         1324 t
ORIGIN      
        1 agataaagga acaacaacaa catcaggaac agttccataa cataagaaag aaacactcct
       61 gagtcagaca catcacagac gggaagggta acgggcgacg cgccagcgtg gcccgcttcc
      121 cttccggcag gcgtcagcct gccaggggtt ttcgcgcggg ctgacaggcg gcctccaccg
      181 cctgcccctg tatcacctgt ttctgctgcc ctgtaagatg tagtgaccgc ccaggaaaac
      241 cgcctgctga ttcgccacaa aatcgcagat ttcatcaaca atctgcgcgg tggtgagcgg
      301 tttctgcccc atttcacgac gccgcgcatt aatcacggcc ctgtaattac gcagaatttc
      361 gcgggtttct gtgctgatat tcatgacatc gtctccccgt ccagcgcctg caacacaacg
      421 tccattgctt cgcgttcctc cctgacgaat gcgtcaccgt cgcccccgtt ctcactcccg
      481 acggcataca ggcgcttcat ttcactgcct gccagcgcca tcactttttc cagcatcacc
      541 gccgggactg tcacatgacg gctggcgcgc ttttcttcca tcagttcacg aacaacgtcg
      601 tgaacaacgc tgacatccgc accatgctca aaggcatcgt ccagtcgctg catgacggtt
      661 tccagctcat cgtctgacag gttccagtcc tgggctatgt gacgcacatc cccacgggtc
      721 agcaccacgg caaaaacgca ttcactgtcc ggcacgtctg tatccagtac atcgcggatg
      781 acttctttaa cagtacagta catgttctcg tctcctttac aggggcggca ttgccgcccc
      841 gtccgttaat cagtccagtg ccgcgtaaat cgcgttgcat tcggggtgaa attcgatgtg
      901 gctccacaac tgcgcatcgc gcatcctgta caggtgtgtc aaccctgcat ccccgctgtc
      961 gtggtacagc cacaactggc ggttaatcac aagggaggta aggatgatgc ctgcggcatc
     1021 agcactcacg gtacggtcaa accagtttgc accgttcacc atgtggaaac ggtcgccatc
     1081 cggcatcatg taaccgccac cgtccggcag gcggatgaag tgccagaatc cgccttcata
     1141 tttcggcatc atttttttcg ccagcgcgta tacagacgct tcggcataca gaaaatccag
     1201 gccaaacagg taaggcagaa aggagagacg gtcgcactct tcaacattca gagtgacagg
     1261 tttcgcatat tgcatgatgt tttcctccgt tgttcacata ttccgcgaca ccgggcggtg
     1321 ccgtgcctcc atctttgccg ggcctgcagg gttgcaaggg cgcaagggaa gccgcagcct
     1381 gacgcgcacg gctgacggga gttggagggt gagaccgcga aatctgagcg ggtgaacgac
     1441 cggaaaatca cggcagacgg gaaggaggga ccgggtgacc gcagcggttc accttacctt
     1501 gcaacccgcc cgcagaagcc cggctacgat ggaggcacgg cacagcccgc acacgacgct
     1561 gaggccacat aactgaaaat ccacaaggcc atgccccgca tggccgggta aagcggagcc
     1621 tgcggcgaca tccttttgct ctttccgggg accggcagaa aaaaagcggg gattttcccc
     1681 gccatcatca gcccctgatt tttgccagag catccaccat gctgcggatt tcatcccgtg
     1741 cctccagaac aacctcccgc agaatatccc ggtcaaacca gcgacgggac ggttcatccg
     1801 gtatacgcag cacatcacag accagaccac tggacaggat atgctccaca cactcactga
     1861 ccagatacat ttccaccgcc gaaaaacgtg ctccccggtt caggatagca tcgagcacca
     1921 cgggcagatt ttcgtcatct tcatcaagcc agcaggggta atcgctttcc ttcacaacca
     1981 gacgaaagcg gcgcgtctgg cgggtcagca acgtctgacc ggtggtggtc agcgccgcac
     2041 ggatacgccg cgcatcttct gccacccggc aactgacact gcatactgca tcgcgcaccg
     2101 catcaagaac cgccctgatt tcttcctccg tcagccattc accggcatac gggaaagaca
     2161 cctgcgttcc gtctgccgga agtaccggca gcgactgcca ctgactgccg gacagaacaa
     2221 gttccgctcc ggttccggtc agtttctgaa tggcagaggc caggagcggc ggcagtacgc
     2281 cgccgtgacg gttgataagt aacgtcgcca gccggggcgc atccagccgc acgtaaatgc
     2341 tgatccagcc cggatagtgc ggattcagtt gttcaatatc cgtctcttcc ggccagccct
     2401 gcgccagcag caggttacgg gcgtggcgat aaaccagtgc attcagcgtt ttgttcatga
     2461 ttaatggtct ccctgaatcg ccgcccggag gcggcgataa catcagtcgt tttcgtgtgt
     2521 gttgtttttt cagcagggcg cgggcctgat taaccaccgc ctgacggcgg gtgacacatt
     2581 ccgggacggc gaggccatga tcccgggcgg cccattcacg tttccacagt gcctgttcgg
     2641 cggtctgaat caggaacgcc agcagttcag ccgggacggt cacctggcgg ttttctctca
     2701 cgttattgat gatctccatc gccacgccgg aagaaatacc ggattcagtc cgctggtctt
     2761 ccgggatgtc ctccaggcgc gccaggacag tcctgatttc atgatcggac agggaaatat
     2821 ccattccgtc agcgagggcc tgaatttctt ccggggacca gataaccagc atcagcggcg
     2881 tgtctcccgg atactgcgct gccagctccc ggcatagcgt ttcgcatgtt ccgtacattc
     2941 tcgtctcctt ctcaatcttt gaagcaggaa tccagttcca gaatttcggt atacgcctcc
     3001 atgaacaggc gattggcgcg atacagcgtc tggcgtgtca ggcgttcaac aagcacatcg
     3061 gaatcaaagc gacagcgatg ctccatatcg ttcagcctgg cccgaaggtc acacatggtg
     3121 ttggccacct ccgccacgtc ggcgcgaagt ttttcgtgtc cggcatagtt catttacgca
     3181 gcctccggtt caaaccagtt atcattcgcg gccccctgct gcatggcccg ttgcacggcg
     3241 gccagtcgtt gctgtccggc acggtgatac tgttcaagca gctcgatacc gatataccgg
     3301 cgtccggact gaagggcggc gacgcaggtt gagccgctgc ctgcaaacgg gtccagcaca
     3361 atcgcgttcg ggtgtgtgaa gctctcaatc agcggttgca ggctggtaac gggcttttca
     3421 gtcgggtgat gacgattgcc tgaatatttc cagcccagca cgtcgggcaa tggcttctga
     3481 ggcagcgccg gacgtccttt tgccaggatg taggcgcatt cgtggcgata gcccacatat
     3541 gcggccttcg atgtgtaggt tttggtaaac accaggtggc caaccacgct gaatcccgca
     3601 tttttccagg cggccataaa gcgatcgacg cggttccagc catagaagct caccattaac
     3661 gcgtcttttt tgagtacgcg gtacatttca ttacaggctg gttgcagcca ttcatcggtt
     3721 ttatcgccgg cgatggtgcg cccggaacgg tcacggaaac cgacgagata cggcgggtcg
     3781 gtgaggatga aatcgacagc attgcccgga aacgtggcca taacacgcac gcagtcaccc
     3841 aggataaaac gggacataac agcactcctt ctcttacaga gggcaccggg agggcacccc
     3901 tgtggtgccc gttccccgct gcctgtgggc tgttcaccgc gtccggtaac gggtcaggcc
     3961 gaccgtggaa taccgcagcc gtcgtctttc tgacggcaag gctatgccgc gaaagcggct
     4021 tgaagggcgt tcatcagaaa agggctcccg gctggcgtac actgtcacca acgccagccg
     4081 ggagcggtaa aagcgcccgt cccgtgcccg gccggacgcg ggacactgac cacaggcagc
     4141 gttcctgccg cgccgcgtca gccggcgcag taaaaagagg agcccggcga catcttttgt
     4201 cacccgccaa acaaaaccca aaaacaaccc acacccaacc caataaaaca ccaaaacaag
     4261 acaaataatc attgattgat ggttaaaatg gggtaaactt gacaaataaa cccacttaaa
     4321 acccaaaaca tacccaaata cacaccaaaa aaaacaccat aaggagtttt ataaatgttg
     4381 gtattcattg atgacggttc aacaaacatc aaactacagt ggcaggaaag cgacggaaca
     4441 attaaacagc acattagccc gaacagcttc aaacgcgagt gggcagtctc ttttggtgat
     4501 aaaaaggtct ttaactacac actgaacggc gaacagtatt catttgatcc aatcagcccg
     4561 gatgctgtag tcacaaccaa tatcgcatgg caatacagcg acgttaatgt cgttgcggtg
     4621 catcacgcct tactgaccag tggtctgccg gtaagcgaag tggatattgt ttgcacactt
     4681 cctctgacag agtattacga caggaataac caacccaata cggaaaatat tgagcgtaag
     4741 aaagcaaact tccggaaaaa aattatatta aatggtgggg atacattcac aataaaagat
     4801 gtaaaagtca tgcctgaatc tataccagca ggttatgaag ctctacaaga actggatgag
     4861 ttagattctt tattaattat agacctcggg ggcaccacat tagatatttc tcaggtaatg
     4921 gggaaattat cagggatcag taaaatatac ggagactcat ctcttggtgt atctctggtc
     4981 acatctgcag taaaagatgc actttctctt gcgagaacaa aaggaagtag ctatcttgct
     5041 gacgatataa ttattcacag aaaagacaac aactatctga agcaacgaat taatgatgag
     5101 aacaaaatat caatagtcac cgaagcaatg aatgaagcac ttcgtaaact tgagcaacgt
     5161 gtattaaata cgctcaatga attttctggt tatacgcatg ttatggttat aggcggtggc
     5221 gcagaattaa tatgcgatgc agtaaaaaaa cacacacaga ttcgtgatga acgttttttc
     5281 aaaaccaata actctcaata tgatttagtt aacggtatgt atctcatagg taattaataa
     5341 tggacaagcg cagaaccatt gccttcaaac taaatccaga tgtaaatcaa acagataaaa
     5401 ttgtttgtga tacactggac agtatcccgc aaggggaacg cagccgcctt aaccgggccg
     5461 cactgacggc aggtctggcc ttatacagac aagatcccag ggcccctttc cttttatgtg
     5521 aactgctgac gaaagaaacc acattttcag atatcgtgaa tatattgaga tcgctatttc
     5581 caaaagagat ggccgatttt aattcttcaa tagtcactca atcctcttca caacaagagc
     5641 aaaaaagtga tgaagagacc aaaaaaaatg cgatgaagct aataaattaa ttcaattatt
     5701 attgagttcc ctttatccac tatcaggctg gacaaaggga acttaatcaa gttatttcat
     5761 tagcaatgat tacataaaat aaatacagtg tcgttattat gaaattattt cacggcactt
     5821 ataaaaaaaa cagtaccaat aatcaaacct ggtgagttcg caatgtctgg caataacgtt
     5881 tttgatgggt tgttttccag cccggagcgc gatattgcca ccagtcacag aaacaccgag
     5941 aacaccagat ttcagcggtc gctgaatttg aacgagatct tttacttgaa cgaacttatt
     6001 cagggatagc aagagcacgg gatgcaggaa aacgttttgg tcgcccggta atgctg
//
LOCUS       DAZPLI010000206         5999 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZPLI010000206 DAZPLI010000000
VERSION     DAZPLI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.208"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..709
                     /locus_tag="ACHUU3_003909"
     CDS             <1..709
                     /locus_tag="ACHUU3_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR2083862.1"
                     /translation="GALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(748..1149)
                     /gene="crl"
                     /locus_tag="ACHUU3_003910"
     CDS             complement(748..1149)
                     /gene="crl"
                     /locus_tag="ACHUU3_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR2083863.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1207..2451)
                     /gene="frsA"
                     /locus_tag="ACHUU3_003911"
     CDS             complement(1207..2451)
                     /gene="frsA"
                     /locus_tag="ACHUU3_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR2083864.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(2543..3001)
                     /gene="gpt"
                     /locus_tag="ACHUU3_003912"
     CDS             complement(2543..3001)
                     /gene="gpt"
                     /locus_tag="ACHUU3_003912"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR2083865.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3262..4719
                     /gene="pepD"
                     /locus_tag="ACHUU3_003913"
     CDS             3262..4719
                     /gene="pepD"
                     /locus_tag="ACHUU3_003913"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR2083866.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(4776..5390)
                     /gene="prfH"
                     /locus_tag="ACHUU3_003914"
     CDS             complement(4776..5390)
                     /gene="prfH"
                     /locus_tag="ACHUU3_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR2083867.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5387..5999)
                     /locus_tag="ACHUU3_003915"
                     /pseudo
     CDS             complement(5387..5999)
                     /locus_tag="ACHUU3_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
BASE COUNT         1505 a         1576 c         1473 g         1445 t
ORIGIN      
        1 gggcgcgttg tatgacgtgg aagcctggac cgatatgttc ccggaatttg gtggcgattc
       61 ctcggcgcag accgacaact ttatgaccaa acgcgccagc ggtctggcga cgtatcggaa
      121 caccgacttc ttcggcgtta tcgatggcct gaacttaacc ctgcaatatc aagggaaaaa
      181 cgaaaaccgc gacgttaaaa agcaaaacgg cgatggcttc ggcacgtcat tgacatatga
      241 ctttggcggc agcgatttcg ccattagtgg ggcctatacc aactcagatc gcaccaacga
      301 gcagaacctg caaagccgtg gcacaggcaa gcgtgcagaa gcatgggcaa caggtctgaa
      361 atacgatgcc aataatattt atctggcaac tttctattct gaaacacgca aaatgacgcc
      421 aataactggc ggctttgcca ataagacaca gaactttgaa gcggtcgctc aataccagtt
      481 tgactttggt ctgcgtccat cgctgggtta tgtcttatcg aaagggaaag atattgaagg
      541 tatcggtgat gaagatctgg tcaattatat cgacgtcggt gctacgtatt atttcaacaa
      601 aaatatgtca gcgtttgttg attataaaat caaccaactg gatagcgata acaaattgaa
      661 tattaataat gatgatattg tcgcggttgg catgacctat cagttttaat ggatattgcc
      721 ggatgtgata catccggcac atttcactca cgccgttaac ttcaccggtt catcacgaaa
      781 atcatccgcc ggttccagct tcagattcag cgtcgtcagc agttcacgca gcttctcgtg
      841 aaactcacgt agggtgtgct ccagtcgttc aaccacttca gtgtctttta ccggaacact
      901 cttccagtcg cctgctttat cgaacagacc aaactggtaa ctgtaggtaa aacgggattc
      961 ctgcgcttca agctccatcc accagcccca gaactcacgc acttccggtg ccggtttcac
     1021 gttgacgcat acagccagac aatcgaaaaa gaatcgatta tcttcgcact taccttcacg
     1081 aatatacggg cctagtgcgg taaatttttt gatcaatctg ctcttcgggt gtccactcgg
     1141 taacgtcatt gcaatctcct gttgtgatgc aactgtttta ccaaattggc aaattttagc
     1201 aaatttttaa cacaagcgtt tttcgatcca gccggtgatt tcctgaagac ctttgtcaaa
     1261 attccgatac accgggttaa atgggatctc taataattta ccgtcagcag atgatgaggt
     1321 gattaagcgt gagtcctctt ccgggctgaa cggatcgttc ttccagtagc ctgataacat
     1381 tggcgttggg cagcgacgcc ccagcaatcc ttgcactttt aatgaatagc gattcagctc
     1441 cacgcgtaac gcttcgtcgg aagcatcatg catccccaaa cgactcgcca gaacgtcaag
     1501 atacatttcc ggcacctgtt gctggcactt aaaatcactc aacagggtat gaactaccgg
     1561 accaagacag gcaaccgctt tcagacgcgg cgattcaaga tatgccagac gcacggcaac
     1621 gttagcgccg aaacggaaac caaaggccgc gacgcgagtg tgatccaccc acggtacgtt
     1681 aggcagagcc tttaaaacgt gctgatgcaa caggctggag tcctgggtga gcttccattt
     1741 tgatgaaaag cccaccgacg gcatatcaat agtcagcatc gcaatgccgc gcggcgcaaa
     1801 ataacgttca tacaggctgt aatagtccgt ctgcatcgca tccagaccac cacacattaa
     1861 tactgtcggg aacgggccat cgcctttcgg catatgcaaa aagccggtga tgggcgcacc
     1921 gccgggtacg gtaaactcca tctgccgcat cgtgcccggc agacgctggg cggcttcttc
     1981 ataggcacgg tttgacaaag cctgcgcttg ctcggccagg tcatctcctt tcagatgagg
     2041 ataggcagca atgttgtaca acgtagccgc atgcagccag tgacgaccac tgagttgcgg
     2101 atcttcttcc gcacaggctt tttgttgcca caccatcgcc tggatggccc actcataaat
     2161 ccagttgcca ccacggtagc caatcaccgt atcgtataaa tcatcgtcgg tacgttcggc
     2221 atcgctcatc acaatacgcg cctggacgtc gaggatttca cgtgggtcaa tgccgcgcca
     2281 gatccacatc agacggttaa tcatgcgata ccagtgaggg atggttttac catcaagggc
     2341 cgactgcaca ggcggttgtg cgccgtgatt aaagcggcgg actagcgtcg aggtttcagg
     2401 atgtttaaag cggggtttga acagggtttc gctcaggttt gcctgtgtca tggatgcagc
     2461 ctccagaata cttactggaa actattgtaa cccgcctgaa gttaaaaaga acaacgcccg
     2521 gcagtgccag gcgttgaaaa gattagcgac cggagattgg cgggacgaat acgacgccca
     2581 tatcccacgg ctgttcaatc caggtatctt gcgggatatc aacaacatag tcatcaacca
     2641 gcggacgacc agccggtttt gcgaagatgg tgacaaagtg cgcttttgga tacatttcac
     2701 gaatcgcaac cgcagtacca ccggtatcca ccaggtcatc aataacgatg aagccttcgc
     2761 catcgccttc tgcgcgtttt agcactttaa gctcgcgctg gttgtcgtga tcgtagctgg
     2821 aaatacaaac ggtatcgaca tgacgaatac ccagttcacg cgccagtaac gcacccggta
     2881 ccagaccgcc acggcttacg gcaataatgc ctttccattg ttcagaaggc atcagtcggc
     2941 ttgcgagttt acgtgcatgg atctgcaaca tgtcccaggt gacgatgtat ttttcgctca
     3001 tgtgaagtgt cccagcctgt ttatctacgg cttaaaaagt gttcgagggg aaaataggtt
     3061 gcgcgagatt atagagatct ggcgcactaa aaaccagtat ttcacatgag tccgcgtctt
     3121 tttacgcact gcctctccct gacgcgggat aaagtggtat tctcaaacat atctcgcaag
     3181 cctgtcttgt gttgacaaca ttttctgcta accctgtgac ctgcaatact gttttgcggg
     3241 tgatcgacaa ggagacttaa cgtgtctgaa ctgtctcaat tatctccaca gccgctgtgg
     3301 gatatttttg ccaaaatctg ttctattcct cacccgtcct atcatgaaga gcaactcgct
     3361 gaatacattg ttggttgggc aaaagagaaa ggtttccatg tcgaacgcga tcaggtaggt
     3421 aatatcctga ttcgtaaacc tgctaccgca ggtatggaaa atcgtaaatc ggtcgtctta
     3481 caggcccacc tcgatatggt gccgcagaaa aataacgaca ccgtgcatga cttcacgaaa
     3541 gatcctatcc agccttatat tgatggcgaa tgggttaaag cgcgcggcac cacgctgggt
     3601 gcagataacg gcattggtat ggcgtctgcg ctggcggttc tggctgacga aaacgtggtt
     3661 cacggcccgc tggaagtgct gctgaccatg accgaagaag ccggtatgga cggtgcgttc
     3721 ggcttacaga gcaactggtt gcaggctgat attctgatta acaccgactc cgaagaagaa
     3781 ggtgaaatct acatgggttg tgcggggggt atcgacttca cctccaacct gcatttagat
     3841 cgtgaagcgg ttccagctgg ttttgaaacc ttcaagttaa ccttaaaagg tctgaaaggg
     3901 ggtcactccg gcggggaaat ccacgttggc ctgggtaatg ccaacaaact gctggtgcgc
     3961 ttcctagcgg gtcatgcaga agaactggat ctgcgcctta tcgatttcaa cggcggcaca
     4021 ctgcgtaacg ccatcccgcg tgaagccttt gcgaccattg ctgtcgcagc tgataaagtc
     4081 gacgtcctga aatctctggt gaatacctat caggagatcc tgaaaaacga gctggcagag
     4141 aaagagaaaa atctggcctt gttgctggac tctgtagcga acgataaagc tgccctgatt
     4201 gcgaaatctc gcgatacctt tattcgtctg ctgaacgcca ccccgaacgg tgtgatccgc
     4261 aactcagacg tggcaaaagg tgtggtcgaa acctccctga acgtcggtgt ggtgaccatg
     4321 actgataata acgtagaaat tcactgtctg atccgttctc tgatcgacag cggtaaagac
     4381 tacgtggtga gcatgctgga ttcgctgggt aaactggctg gcgcgaaaac cgaagcgaaa
     4441 ggcgcatatc ctggctggca gccggacgct aattctccgg tgatgcatct ggtacgtgaa
     4501 acctatcagc gtctgttcaa caaaacgccg aacatccaga ttatccacgc gggcctggaa
     4561 tgtggtctgt tcaaaaaacc gtatccggaa atggacatgg tttctatcgg gccaactatc
     4621 accggtccac actctccgga tgagcaagtt cacatcgaaa gcgtaggtca ttactggaca
     4681 ctgctgactg aactgctgaa agaaattccg gcgaagtaat tatttgattt gctgccggat
     4741 ggcgtttaat cgccttccgg cagtttcatc cttcattatc cttcgataaa agccatccct
     4801 gtaaatgtcc gtctcgggtt gccacgttca atctggtgat ggaacattcg ccgctgcgat
     4861 ttcagcaccg cgctattttc ctgttgctgc tgctccagct tccaggcaat cagcaatcgt
     4921 gccagccgct tgttagcatg ctgactacgc tctgactgaa ccttcacgct aataccggat
     4981 gccaaatgcg tggcgcgtac cgccgagtcg gttttattga catgttgacc gcccggcccc
     5041 gacgaacgca acgtctcata acggattgca tccgattgtt cctgctcatc agcggtaaaa
     5101 cgcccaatgc ccagaaacca gtttttgcgc ccatgatgag gccgatacgg actcggacaa
     5161 atccactgaa tagtgccgca ccagctttcg cttaatgccc atgcgttatc gccatccaga
     5221 gaaaccagag ctgaacgcag cgtgtcagag tagcggcccg tttctgtttc caacaccgtt
     5281 accgcgacgt cttgtcgggc agcttcttta attaatcgct ccagcgcctt tttcactgcg
     5341 agacaacatt cttccggccc ctgcgctgag gagagttgta gcaagatcat ccttttttcc
     5401 ctccactgtt tttgagcgtt agcaccggac gcagccgcgc gacaggaata attaaaccag
     5461 cctgcaccag acattgcacc acgctttcag ccgatttata ggcttgtggc gcttcttcaa
     5521 agatgagttg tttatcgcga caaattaccc gactgccaag ttcagtacgt gagagctgcg
     5581 tcgctgtgta tttcgctgcc agacgccctt tacactcggt gcgcccccat ttacgcccag
     5641 ccccatgcgc cagcgagtgg agcgtttttt cgttcgcaac aggctgtaca agctaggagt
     5701 aatcaccgag tgaaccggga ataatcacca gaccgttgtc atccggcgtg gcacctttac
     5761 gatgcaacca gccctgttga tcgccgattt gacacgcgct aacaaagtta tgcgccacat
     5821 ccagaaccgg acttccggtg gccttaacct gttgcattat gcgtaaagag atcagctggc
     5881 gattaatacg cgcaaacgcc agcgcatcgt catgttccgc aatataccgt agcgcgtcgt
     5941 cactgccttc aggcaaacca tgatgcgaaa acgaggcaat atgccgctgt aaaatagac
//
LOCUS       DAZPLI010000207         5931 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZPLI010000207 DAZPLI010000000
VERSION     DAZPLI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.209"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            292..368
                     /locus_tag="ACHUU3_003916"
     tRNA            292..368
                     /locus_tag="ACHUU3_003916"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHUU3_003917"
     CDS             complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHUU3_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR2083868.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="ACHUU3_003918"
     CDS             complement(1357..2247)
                     /locus_tag="ACHUU3_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR2083869.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYIRAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHUU3_003919"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHUU3_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFR2083870.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..>5931)
                     /gene="nfrB"
                     /locus_tag="ACHUU3_003920"
     CDS             complement(5207..>5931)
                     /gene="nfrB"
                     /locus_tag="ACHUU3_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HFR2083871.1"
                     /translation="DTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESID
                     AWQIPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRY
                     FIVLRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAE
                     ILTTLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQS
                     LLLKAGLNTEQVAQLESENEGE"
BASE COUNT         1346 a         1561 c         1549 g         1475 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg ccctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagcg cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg a
//
LOCUS       DAZPLI010000208         5917 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZPLI010000208 DAZPLI010000000
VERSION     DAZPLI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.210"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(1..1133)
                     /locus_tag="ACHUU3_003921"
                     /pseudo
     CDS             complement(1..1133)
                     /locus_tag="ACHUU3_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHUU3_003922"
     CDS             complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHUU3_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfR"
                     /protein_id="HFR2083872.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1327..3060)
                     /locus_tag="ACHUU3_003923"
     CDS             complement(1327..3060)
                     /locus_tag="ACHUU3_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR2083873.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3057..3551)
                     /locus_tag="ACHUU3_003924"
     CDS             complement(3057..3551)
                     /locus_tag="ACHUU3_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR2083874.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3673..4023)
                     /locus_tag="ACHUU3_003925"
     CDS             complement(3673..4023)
                     /locus_tag="ACHUU3_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR2083875.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4066..4530)
                     /locus_tag="ACHUU3_003926"
     CDS             complement(4066..4530)
                     /locus_tag="ACHUU3_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR2083876.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="ACHUU3_003927"
     CDS             complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="ACHUU3_003927"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFR2083877.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(5024..5239)
                     /locus_tag="ACHUU3_003928"
     CDS             complement(5024..5239)
                     /locus_tag="ACHUU3_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR2083878.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT         1358 a         1699 c         1431 g         1429 t
ORIGIN      
        1 gggacgcgga ttcatatctt ccaggtcgcg gcagtcattg gaagagtaaa ttccccagtt
       61 gatcccggtg gcgtaggctt caaaacggga cttcatatcc ccgcgcagta acgccccggc
      121 gttaaatttg gcgtaataaa cgccctgctt actttttcgt accagtccgg tgttgatccg
      181 ctgttcgatg cgggtcagat acggcaccag tgaatagttg ataaatccca gccccagctc
      241 ttcgatattg ttgaaggtgg cgcgatcggt gttctgcacc atgtgcaacg gcacccggaa
      301 cagacgacag atttcttcca gctgaaactt gcgggtttcc aggaactgac tgtcctcggc
      361 gttcagcgcc atcgacttcc agtccagccc catttccaga atcatcgggc ggtgagcatt
      421 gccaagcccc gtgtgacact cctcaaaatc tttcttcaga cgctcgtaag catccggcgt
      481 gagcttttgt tccgtacgca acacaccgga tgtcaccgca ccattactaa acaacctggc
      541 accgtgctcc tcggttgccg ctgccagtga aatggcctca cgcgcatacg caatgggatt
      601 cagcccgaca agtccatcca gcgtcagggt gcgcacatgc cagatttcat tctgggtcaa
      661 cacatccacg gaaccatccg gaaacgtcac ctgataaacc ggctgccact ggctgttcag
      721 cttcggttcc acacagcccg gatctatcgg aagaagctcc accacttctc ccagtgcctt
      781 taccttgtag gcgtaaaaat tcccccgtag acacaggcag acaataacca gctcccagaa
      841 ttcctgcggt gtcatgtagc cattaggttt tgccgaaacc aacttatgca gtcgttcatc
      901 caccgcccgt gttttcaggg taccgctgat tttgtagaga ctgcagggca gcataccaac
      961 agactctgcc agcaccctga cgcaagaata gaccgccgtc aggcgcatgg cccgctggct
     1021 actgatccgc tttccggtat aggtgtcgta tgacagcccg atagcatcag cagttctgct
     1081 ggcgtggtca ccggcacatc gctttttcgt tgaaataatc ccgaaaagaa cactatttac
     1141 ctccgccgac agactgccgt gtacggtcga gatatcgcgc caccagccac gaccagaaca
     1201 ggcacagcgc cccggcaaca acaaaacccg ccgggggata aatcagccag gcaccatacg
     1261 ccagcaaaag cgcacccagc acgcccacca gaggcgcgag aatcagcatg atcataatta
     1321 cctcagttaa agcgagcgga tcccgtagga ctcaatgtgg tcagacagcg tgtcttcttt
     1381 ctcgtacagc atggctctgc caaccgccat aatcagcgca actgcaccat cgattttgtt
     1441 ttccgcctgc tctttgacgg gtttcaccac atcatcgtta cccggcatgt ttttgccgac
     1501 cacgttgccg atacaccagg tcatgatggg attgccgtca tgatgaaaac gtcccgattc
     1561 aatcgctgct tccagctctt tcatcggatc ggacatattg gtgaagttct ggacgatagt
     1621 gacgggattc aggtcttcat cagcaaggtc atgtgacaac ccggtcgccc cgaaggggtc
     1681 gatgggtgac tcactgaccg ggctgatttt gttcgccgct ttggcctcct cgaggatgta
     1741 gcgataatcc acctccgcac catcggtaac ggtcagaacg cccatttcca cccatttctg
     1801 aaagcgttcg gctgtccgtc gatcttcatt tttctcgacg ctgtacaccg tgtcatacgg
     1861 tacccagaaa cgcggggcca cactgtagta atgcgtttta ccgtcaatct cgcgggtata
     1921 aagtcgcgcc atgctgttca tatccagctt acgcgccagg tcaaaggcca gaatgcacgg
     1981 ctgcccctcg aactgctcaa gggtcagtga tttatcctcg cagctctgcc agctcaccaa
     2041 gttgaaatac gccgaacgcg ccgacaccca gatattgagg tgttttgttt taaagacgtt
     2101 tgccagacgg gcgttatttt tcgcacgctg ctgctgactt aacaaaaatt cgcgataaac
     2161 cgacacgcca atatttggat tggctttttc cagcacctgc gggccggtcc agtcgtcacc
     2221 ttcatcaacg gtatagatga tcccgaacag ttcatcgtta ggcaccgagc cgttgagcat
     2281 ctcgatgact tcccgccgct tgtcgtagca cggcccctca atgttgtacc cggcggtggt
     2341 gatggcccac atcagtggct gacgtcgcgc ccccatcccg gtaagcattg tggtataaag
     2401 cgcatcggtg gcatgctcgt gatattcatc aaccacggca cagtggggtg atgaaccatc
     2461 acctgggttg ccgatcagcg gttcaaaccg cgcgccatcc tccggacggt tcatgtttga
     2521 ggcgttaacc tcaatcccga acgcttccgt cagcatgggt gtgcgtttac acatcagtcg
     2581 cgccgggcga aagacttccc acgcctgttt ctctgtcgtg gcaccggaat acacttccgc
     2641 gccaaactcg ttatcacagg caaaacaata cagggcaaca ccggcagaga ttgctgattt
     2701 gccgttctta cgggggattt cggtgtacac ctcccggaag cggcgcaacc gggtgccttt
     2761 attgacccag ccaaacgcac agcagatcac aaatagctgc cacggctcca gcgtgatggg
     2821 catccgtttg aatgcccact cccccttggt gtgcggcaac agctgaataa atttcgcggc
     2881 ccgttcagcc aggtccttgt cgaagcggta acgaaacgac ttactttttt ccgccatcag
     2941 gtcatcaaga tggcgctggc aggcctgaat cacaaactgg caggcaacaa tctttccgcg
     3001 cacgacatcc cgggcatact gatttgcagc atttacgttg gggtaagatt tccggctcat
     3061 gattcgataa ttttcagaaa cgggttagtg gctttcttct gccccgccag gccaatcaga
     3121 cgctggcggc tgctggggtc gagtccgagc attgcccccg tgctgctcat ctcggactcc
     3181 tgttcttttt tggcggtcag ctccggattt ttgaccctgc cgcccattgc accggtgatg
     3241 gtgttgccct gtatggcaat atttttcacg gcacgtcgcc agaactcata ggccacgcac
     3301 caccgctcaa gtaccgccag gtcagtcacg cacagcaggc cctgaccgca gagttctttg
     3361 gttgtcagtt gccacatgat cgtggcgaga gggagatctt cttcagcgaa ccactctggt
     3421 ggctcaacac ctttgatggg cgtaaaaaca ggttcatctt tgttcagggc tcgcttgccg
     3481 gggtttccgg ccagcgcctt gcgcgccgtt ggcttggggc gacgcccgga acgccccgcc
     3541 gttccagcca tatgcggcac tcctggttaa atttcatttt tcgcgggtat aaaaaaacga
     3601 tggggcgggc agtccggaag acgtcaggtc acagggattt gccccgcccc tccctacaag
     3661 tgagaataat tatcaccgga ttcgttcgcg cgctgttttc gctttatggc agggccagca
     3721 cagactctgc aggttgctgt ctgcgtctgt tccgccatgc gctttcggga tgatgtggtc
     3781 gacggttttc gcctcgctca ccacaccgac acgcagacac aactgacaca gacctttatc
     3841 gcgcttcaga atacgggcac gaatcaccgt ccattttgag ccatagccac gctggtggcg
     3901 gctcagtccg cgctggtgct gcacccagcc ttcaccgcga tgtttatcgc agtaacccga
     3961 actgtctgtg gttgtacctg cacatccacg cttacggcag gcacgtggga ttagtgatgg
     4021 cataaatacc tcataccctg cgaaatgttt accacgataa aaaggctact taatgcactg
     4081 agtgcggata tactcctgtg ccccttccag ttgcatctgc atcgtcatca gccgctctct
     4141 gagggtgaaa taatcccgtt cagcggtgtc tgccagtcgg gggctggttg cattatccac
     4201 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg
     4261 acgaccagcg gcaacatcag cgcgcagagt ttcattttca gctttcgcat cagctaactc
     4321 cttcgtgtat ttagcatcga gtgcattaac atcacgctgt ctctgctgca tgtcagtaat
     4381 ggttgcgttc gccagcttca gttctcttgc gtttttgtcg cgctgctctt tgtaggtaat
     4441 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
     4501 agcggagata atcgcggtga ctctgctcat tgctgccccc acaaacagac ttcacgctca
     4561 atctcacgac gggtcatcag ccctttccat tgcttaccgc cagcatatgt ccagcgacgt
     4621 agctggtcac atgcgccttt gatatcgccc tggtttattt tgcgaagaag cgtcgatgtt
     4681 ctgaaattgc cagcgcccac gttgtaaacg aacgagtaaa gagcgccgcg cgttgtttcc
     4741 ggtatatcga ctttgatgta cgggttaatt tgtctggcga cagtggcaag gtctttattc
     4801 aggagggctt tgcattctgc ttcggtatac gttttaccga gcatgatgtc ttttccggtg
     4861 tgtccgtgac atacagtcca tacaccaatg atatctttgt atggtatgta gctgacacct
     4921 tccagaccat cgttaccacc tgggccagtg attaacacag atgctatagc aacagcccca
     4981 ccaccaatag cagctgcaac agccttgcgt aatgatggcg acattattca cctctcgcag
     5041 ccttacgctt gtcttctctt attttgaaat acagatttgt cagataagtc agaagcccca
     5101 gaagcagact tcccagcaca ccaatcgcag cccactgtga tggactgacc tgatccaacc
     5161 actgcaaaaa ccagtagccg gcactgccgg cggaggtgcc gtaggcaatg cctgttgata
     5221 ttttttccat ctgatacata tcccgccccc cgacagacct gtgctatcgg aaagaaaaaa
     5281 ggccatcagc aaaactctga tggcctgaat cacctttacc aatattgtat gaaaaaacac
     5341 gcacgactaa ttgacaataa ttttcatttc cattaaaaaa gatcgcgtag cattcttaat
     5401 tcatgaggaa ctttacccgc cagcaatctg agtagcgaaa gctgttcatc cccaatgttt
     5461 ttgctggcgg gtcctttttt ctacggtcct cctccccgga ggggggcatt tcattatttt
     5521 tctaatcgtt actggtgaga aggcggaaga aaaagccagt tcttcgcccc taaataacag
     5581 catgattcca gtccatgcaa aatctgattt ccagctcaca cataccgagc gctgtacaaa
     5641 aaaccgccag ccaaggcagt taagagtgtg ttcccggggt ttgcttagaa tattttgtaa
     5701 gttgtccgaa gagatattta caacaccaga atgatgaatc atcagtcccc tgccagaggt
     5761 tcactacgta acctgaccga caatgcacca ttctggtgct gaaaaaagag cactggaact
     5821 gcaacaaaac aacatcacag aacaggagat ggtaaggagt cagacattcc cacacaatat
     5881 tgtgtcagtg caaataacaa ccttcgtctc aaatctc
//
LOCUS       DAZPLI010000209         5901 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZPLI010000209 DAZPLI010000000
VERSION     DAZPLI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.211"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..1484
                     /gene="tssI"
                     /locus_tag="ACHUU3_003929"
     CDS             <1..1484
                     /gene="tssI"
                     /locus_tag="ACHUU3_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR2083879.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASDLHGEQPQAVPGRRGSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIETVGVNQIE
                     TVGSNQIIKVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRV
                     GLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGT
                     KIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            1552..5547
                     /locus_tag="ACHUU3_003930"
                     /pseudo
     CDS             1552..5547
                     /locus_tag="ACHUU3_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT         1408 a         1498 c         1910 g         1085 t
ORIGIN      
        1 tctgcatcag ccagtttcgc tacagcgcac aaatccgtcc ttcttccgta gtgaccaaag
       61 actatacctt taaacgtccc ggctggccag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacgcagtat gaagtgtatg actaccccgg acgtttcaag ggtgcccacg
      181 ggcagaactt tgcccgctgg cagatggacg gctggcgaaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttcgccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcgagcga tcttcacggc gaacagccgc
      361 aggcggtacc gggacgcagg ggttcaggca ccacgctgga gaaccatttt gcggtaatcc
      421 cggcagacag aacatggcgg ccacagccgt tgatgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggct ttggcaacct ggcgataccg cgtgtgggtc
      661 aggaggtgat tgtggacttc ctcaacggcg acccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggca gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgcgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgctg aaaccattgg caacaatcag atgattgcgg
      961 ttaccaacaa tcagatagag acggtgggcg ttaaccagat agagacggtg ggcagtaacc
     1021 agataattaa agtgggttct gttcaggtgg aaacgattgg acttgttcgt gcgctgacgg
     1081 tgggcgtggc gtatcagacg acggtaggtg gcattatgaa cacctcggtg gcactgatgc
     1141 agtcctcgca gatcggtttg cataaatcgt tgagggtcgg gctgggttat gacgtcaaag
     1201 tcggaaataa cgttaccttc accgttggta aaacgaaaaa ggatgatacc gggcagaccg
     1261 cgatttactc cgccggtgag catctggagc tctgctgtgg taaggcaagg ctggtgctga
     1321 cgaaggacgg acaaattttt ctcaacggca caaaaattca tttgcagggt aaggagcagg
     1381 ttaatggtga ctcactgttg attaactgga actgtgcagc ctcgaaatcc ccaccgaaga
     1441 cccctgatga aaagcaggat acgccggata tgagagagta ctgatcccgt acctgttcgg
     1501 tattcagtaa caaaccagca ttaaaaataa acagaagaag gaatttgaat catgagcgga
     1561 aaaccagcgg cgcgtcaggg agatatgact cagtatggcg gtcccattgt ccagggttcg
     1621 gcaggtgtaa gaattggcgc gcccaccggc gtggcgtgct cggtgtgtcc gggcgggatg
     1681 acttcgggca acccggtaaa tccgctgctg ggggcgaagg tgctgcccgg cgagacggac
     1741 cttgcgctgc ccggcccgct gccgttcatt ctctcccgca cctacagcag ctaccggacg
     1801 aagacgcctg caccggtggg cgttttcggc cccggctgga aagcgccttc tgatatccgc
     1861 ttacagctac gtgatgacgg actgatactc aacgacaacg gcgggcggag cattcacttt
     1921 gagccgctgc tgccggggga ggcggtgtac agccgcagtg agtcaatgtg gctggtgcgc
     1981 ggtggtaagg cagcacagcc ggacggccat acgctggcgc ggctgtgggg ggcgctgcgc
     2041 cggatatccg gttaagcccg catctttacc tggcgaccaa cagcgcacag gggccgtggt
     2101 ggatactggg gggggctgag cgggtgccgg gtgctgagga cgtactgcca gcgccgctgc
     2161 cgccgtaccg ggtgcttacc gggatggcgg acgctgacgt accggcgtga ggccgccggt
     2221 gacctggccg gggaaatcac cgacgtgacg gacggtgccg ggcgggagtt ccgtctggtg
     2281 ctgaccacgc aggcgcagcg tgcggaagag gcccgcacct cttcgctatc ttcttctgac
     2341 agttcccgcc ctctctcagc ctcagcgttc cccgacacac tgcctggtac cgaatacggc
     2401 cccgacaggg gtatccgcct ttcggcggtg tggttgatgc acgacccggc atacccggag
     2461 agcctgcccg ctgcgccact ggtgcggtac acgtatacgg aagccggtga actgctggcg
     2521 gtatatgacc gcagcaatac gcaggtgcgc gctttcacgt atgacgcgca gcacccgggc
     2581 cggatggtgg cgcaccgtta cgcgggaagg ccggagatgc gctaccgcta cgacgatacg
     2641 gggcgggtgg tggagcaact gaacccggca gggttaagct accgctatct ttatgagcag
     2701 gaccgcatca ccgtcaccga cagcctgaac cggcgtgagg tgctgcatac agaaggcggg
     2761 gccgggctga aacgggtggt gaaaaaagaa ctggcggacg gcagcgtcac gcgcagcggg
     2821 tatgacgcgg caggaaggct cacggcgcag acggacgcgg cgggacggcg gacagagtac
     2881 ggtctgaatg tggtgtccgg cgatatcacg gacatcacca caccggacgg gcgggagacg
     2941 aaattttact ataacgacgg gaaccagctg acggcggtgg tgtacccgga cgggctggag
     3001 agccgccggg aatatgatga accgggcagg ctggtatcgg agacatcgcg cagcggggag
     3061 acagtacgct accgctacga tgacgcgcac agtgagttac cggcgacgac aacggatgcg
     3121 acgggcagca cccggcagat gacgtggagc cgctacgggc agttgctggc gttcactgac
     3181 tgctcgggct accagacccg ctatgaatac gaccgcttcg gccagatgac ggcggttcac
     3241 cgcgaggaag gtatcagcct ttaccgccac tatgacaacc gtggccggtt aacctcggtg
     3301 aaagacgcac agggccgtga aacgcagtat gagtacaacg ccgcaggcga cctgactgcc
     3361 gttatcaccc cggacggcaa ccggagcgag acacagtacg atgcgtgggg aaaggcggtc
     3421 agcaccacgc agggcgggct gacgcgcagt atggagtacg atgctgccgg acgtgtcatc
     3481 agtctgacta acgagaacgg cagtcacagc gacttcagct acgatgcgct ggaccggctg
     3541 gtacagcagg gcggctttga cgggcggacg caacgttatc attatgacct gaccggaaaa
     3601 ctcacgcaga gtgaggatga ggggcttgtc accctctggc actacgatgc gtcggaccgc
     3661 atcacgcacc ggacggtgaa cggcgacccg gcagagcagt ggcagtatga cggccacggc
     3721 tggctgagag aaatcagcca cctgagcgaa ggccaccgtg tcgctgtcca ctacggctac
     3781 gacgataagg gccgcctgac gggcgaacgg cagacggtgg agaacccgga gacgggggaa
     3841 ctgctgtggc agcatgagac gaaacacgcc tacaacgaac aggggctggc aaaccgcgtc
     3901 acgccggaca gcctgccgcc ggtggagtgg ctgacctacg gcagcggtta cctggcgggc
     3961 atgaagctgg gcgggacgcc gctggtcgag tatacgcggg acaggctgca ccgtgagacg
     4021 gtgcgcaccc tcgcaccgga cctggacatc cgcatcccgt atgccaccga cccggcggga
     4081 aaccgcctgc cggacccgga gctacacccg gacagcacgc tcacggcgtg gccggataac
     4141 cgcatcgcag aggatgcgca ctatgtttac cactatgatg aatacggcag actgacggag
     4201 aagacggacc gcatcccgac gggtgtgata cggacggacg acgagcggac ccaccactac
     4261 gacagccagc accgcctggt gttccacacg cggatacagc atggcgagcc actggtcgag
     4321 agccgctacc tctacgaccc gctgggacgg cgaatggcga aacgggtatg gcggcgggag
     4381 cgtgacctga cggggtggat gtcgctgtcg cgtaaaccgg aggagacatg gtacggctgg
     4441 gacggcgaca ggctgacgac ggtacagacc gacaccacac gtatccagac ggtatacgag
     4501 ccgggaagct tcacgccgct catccgggtc gagacagaga acggcgagcg ggagaaagcg
     4561 cagcgccgca gcctggcgga gaagctgcag caggaaggga gcgaggacgg gcacggcgtg
     4621 gtatttccgg ctgaactggt gcggctgctg gacagactgg aggaagaaat ccgggcagac
     4681 cgcgtgagca gtgaaagccg ggtgtggctt gcgcagtgcg ggctgacggt ggagcaactg
     4741 gccagacagg tggagccgga atacacacca gtgcgaaacg ttcatcttta ccactgcgac
     4801 catcggggcc agccgctggc gctcatcagc gaagacggcg atacggtgtg gagcggggcg
     4861 tatgatgagt ggggcaacca gctaaatgag gagaacccgc atcatgtgta tcagccatac
     4921 cgtctgccgg ggcagcagta tgatgaggag tcggggctgt actacaatcg tcaccggtac
     4981 tatgatccgt tgcagagacg gtacatcacc caggacccca ttgggctgaa aggtgggtgg
     5041 aatctgtatc agtatccgct gaatccggtg agcaggatag atccgttggg gttattctca
     5101 tgtgagagtt gtagtcattt tatagatgat ataaataata aactggataa ccttccgttt
     5161 aacccgacaa aaatgtttac agctagttta aatactgcta attcagcaag gctgtatgca
     5221 agtggcatga caaaattcgg tataagtgtt gctgccgatg gaacgatcat tgctgcccca
     5281 gtcggtgcag cttctatggc tttaggggcg tggaatataa aatctgcgac agctgcatat
     5341 aacagagcaa atttacaatt atcagaatca cttcaggaaa atggtagaga taggtgttgg
     5401 agaaatttat tgggtatttt accttatggt gagcagtttg atgaccctgg tgaaccatca
     5461 atattagacg tttatggtga aaagattgag aatatcaaag attctccaat gaagtttttt
     5521 gaagagcttg ggacgctggg gttgtaatgt gtatttttaa tgatattttt atgtttgtgt
     5581 ttattgttag tagtatactt gtaaccgtgc attttgtgta ttctttattt tttaaaagtg
     5641 gaaaggtaaa agataaggta aacaaatgga ttaaagtaat gtgtgattta ttttggggga
     5701 taggatagtg gtgtgatatg gataaatata aattacagtg gctggcatat ttcgacctga
     5761 cgccggagta gataaaaaaa cagacaggat tggagtatag caagcgtctg acaatctctc
     5821 gggacagtag tatgatgaag aggcggggtt gtactataac cgtaaccggt actacgatcc
     5881 gctaaagggc aagtatatga c
//
LOCUS       DAZPLI010000210         5886 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZPLI010000210 DAZPLI010000000
VERSION     DAZPLI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.212"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..1255)
                     /locus_tag="ACHUU3_003931"
     CDS             complement(<1..1255)
                     /locus_tag="ACHUU3_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR2083880.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLMERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKILSKDVLPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLL"
     gene            complement(1412..1621)
                     /locus_tag="ACHUU3_003932"
     CDS             complement(1412..1621)
                     /locus_tag="ACHUU3_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083881.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1644..2354)
                     /locus_tag="ACHUU3_003933"
     CDS             complement(1644..2354)
                     /locus_tag="ACHUU3_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR2083882.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2870..3193)
                     /locus_tag="ACHUU3_003934"
     CDS             complement(2870..3193)
                     /locus_tag="ACHUU3_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083883.1"
                     /translation="MNEYELQIFIDSDTAMMVQAFADSGVSIDLDKLLKLMAENSEEI
                     EDFIQEIETGEPRFMFPVTDSNMKRLIIEETNKYSVSPEKYLKAAIAILYSDNILVTD
                     SKVVH"
     gene            complement(3372..3572)
                     /locus_tag="ACHUU3_003935"
     CDS             complement(3372..3572)
                     /locus_tag="ACHUU3_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083884.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(3572..3844)
                     /locus_tag="ACHUU3_003936"
     CDS             complement(3572..3844)
                     /locus_tag="ACHUU3_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR2083885.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(3847..4125)
                     /locus_tag="ACHUU3_003937"
     CDS             complement(3847..4125)
                     /locus_tag="ACHUU3_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083886.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(4126..4683)
                     /locus_tag="ACHUU3_003938"
     CDS             complement(4126..4683)
                     /locus_tag="ACHUU3_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083887.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
BASE COUNT         1686 a         1230 c         1024 g         1946 t
ORIGIN      
        1 taagtaaatt agtcagtgac cttgtaaagt ctgttaaacc ttcctgtgaa ccatcgaaca
       61 ttaaggccag attattattc catgaattac ttaagcggcc taactgcgtc tgagtagagt
      121 taataatctt ttcccatcca ccagctttat caatcatcct tcccatttca gcggatactt
      181 taggaagaac atctttagaa agaatcttac cgtctttcat ggccttgtct aattcttccg
      241 tagtcatccc aagacttctg gcaaataact gaaaagcacc tgccattctg tcacctaatt
      301 gaccgcgtag ctcttcagcc tgaatctttc ctttagatgc catctgctcg aaagctttta
      361 aaatcccgtt aatttcatcg gtgcttagcc ccattaatct ggatgtacca atcacattag
      421 aaaataagga ttctttctgg tctgcattca tatcaccagc accagcggaa aacttagcaa
      481 aactgcttaa tgtttcaatc tggtctacac cactactatc agcaagattc ttaacaaatt
      541 ctgtagctcg tttagcacct tcatcaccta aaacgaattg tgccgctcgt tgtgatgctt
      601 cttttttcag accaacctcc ataatccgcc tgtaagcttc taaggctgtc tgaaaactat
      661 aaatggctgt acctgctttg attgcactcg tagctaatac atccagacta tttgaagcat
      721 tttcactgtg attatttttg ctggtggtct tgttaaactt agaaagtgtt tcacgaagag
      781 actgatttaa ctttgctaac tcacgattaa aagtttcgtt tccaagtgtc ttggatttac
      841 tgcttaagct gtaatactgg cttttaaaat acttctcaag cataggatta aagtcacgaa
      901 gcttgctcat attcgctgag taagctgctg tgattgctct gcttccgcta attcgtggct
      961 gtcttggttg tctaagtggc tttaaagtat cctcaatttc agttttaatc ttaaacgctc
     1021 tagtgttttt aagacgctgc tcaatcttgg aaacagtttt atcgacattt ctttccatta
     1081 gtttgagaga atcatttaaa ggcgtaggat taactctaaa cgacttattc aatcgcttct
     1141 caattcgtga tgctacctgc atcgtttgtt tctcggcttt ttccaagcct tttaaaactt
     1201 tctgagagtt aaaacttaac tcaatcagaa aatcatcaac tttaaattgt gccattcata
     1261 tttttcacct ctttaagcgt gttgtacgta aattctattt ttcagtgtgg gtattagctt
     1321 tacagatttc acgtagcaca catacaaaaa aagggagagc taccgatgtc actcgataat
     1381 ctctccccgt atttacccaa ccaattcttt tttaatgtcg gacaagataa tgtaattccg
     1441 tttcacctat gatttgagca tcagaaggaa tacggtcaat acaccacttt ggaatcttgc
     1501 gtgtttttgg tttgtgtgag gtgataactt gacgtttgtt tttaattctt aaggctggct
     1561 tagtttgtaa ctttaagttt tcacaaacag accgaatagt ctctaaacgt tcttcaaaca
     1621 ttaatcacct ctttaattca taatcaaaca gccttgctat acttctgaga aatcttcgtt
     1681 aactgctcaa tatcagccat caaagcaatc ttcttctgcg actcatattc actaacaagt
     1741 tctttcatag tatcaagttc ttttttaagc ttttgcttta acttatccag tttaccggaa
     1801 taatgcttag tcaaattagc ttcagctaac tgaagaatct cttcatctgt tttatcagtc
     1861 atcagaataa attgaccaga accccttaat gcttgtctga catcaattaa ccggaaccct
     1921 tcagggatac tttgaagaac tttaggaacg tcttcaatcg ctgtaccaaa accatcatta
     1981 aagtttcctt cataatagat aaacgggttt gacttaatct cgtaagcaat atcatattct
     2041 gaaggtttcg aggtttcttc gaagttctta acagcatcgt aagcgacatc aagtctaaga
     2101 cgctgtgatt ttaaagctgg gtaatattca cgctcaagct caaatagtgc ttcagttttg
     2161 cagatatcag gcatttcttg aggctcagga gacacaaagg aaattacctt tggtgacatc
     2221 tttagctctt gctcaagctc aatgcgcttg tcatgctctt gacgctgaat agtatgtagc
     2281 ggatgttcac cctgtggtga ccagtctccg aagctatcaa cctttttacc gttaatatat
     2341 aaatcgtttg tcattataat ttcctctaag tgtggttatt tcttaataga gcaccttcaa
     2401 cgccaataca cttagaagat gacttaacga tttattaaag atgctctatt aagaaaagcc
     2461 ctaaacataa tctgtgaagg gctttggagg tttgcttata acgttaacga agtttcagga
     2521 aaactttctt taaaagctct gtttaaaaac tcttaaagaa agccctaact catgattagg
     2581 gcaaagctgt aaaggattca ataatgaatg aacaaagact ttttgagata gcctctcaga
     2641 ccaacctgaa aggctaggac gaaaagcatt gcacctttcc aactcagaat atccatccat
     2701 cggattattg aaagaaaaaa taagagagaa ttgctctccc ccatatatac aattatgtca
     2761 tgctcaaggt tgtttgtcaa cagcttttaa cagattggta acatttatac tttaatagat
     2821 gcacctgcaa tcataccaat caatacaaaa cagataatca ccaattccat taatgcacca
     2881 ccttcgaatc cgtcaccaga atattatcgc tatacaaaat tgctatagcc gccttgagat
     2941 atttttcagg gctaacgctg tatttgttcg tttcttcgat aataagacgc ttcatgtttg
     3001 agtcggtaac aggaaacata aaacgcggtt caccagtttc aatttcctga ataaagtctt
     3061 caatttcttc tgagttttca gccattagct ttagtaattt atcaaggtca attgatacac
     3121 cggaatcagc gaaagcctgc accatcatag cagtgtcgct atcaatgaaa atctgtaatt
     3181 catattcgtt catttttaaa atctcctttt taccagacct cctcaatcgc tctgtaacga
     3241 gctaaacagt ctttttcagg ggtagcctgt acagtggtat tacccaccct aaaataagat
     3301 agttttttgc gttagaatgc gattgagagc gtctgataag ctgtttttct aatttctctt
     3361 aaagtctcgg actaccaata acctaattcc gctttatttc gttcaaacca ttccgctgta
     3421 actgctgtct ttagtgttgc gtttgggctg gtggcatgaa tgaagatatc ttttggattg
     3481 caagcatcac cgaaaccacc ttctttgatg agcaggtctg atacatgctt aatcatttta
     3541 tctgtcaaat attcgaattt aatacataac attaaatctc caggagtaaa ttgtttgggt
     3601 ggcaatttct tttattacca tccctgaaga taatcttttt agttgcctga acttcatgat
     3661 gcaaaaacca gatgacatac ttcagataat aatcacgagt gccataacga atcattggat
     3721 taccgtaagc atcatctctt gtcttaacct ctccaccatc aatccattca agcattcctg
     3781 tctcataatt aatcatgaag taagttgcaa agaaccgttt aatatcatct cgccattgct
     3841 tcataatcag acctcctgag acagtttgtg aattttgata cttagtaacc ttgctaaatc
     3901 cagtccaaaa gcatttaagg atttcagact atcaaggtca tcttgcttaa tcctgatatg
     3961 gtgtcttgca gcctctaagc gactattaag caaatcctgt tcgttaatat cacggttaat
     4021 tgcaagcttc atcactgctg agtgtggcag acctgtaatg tttttgattc ggttcagttc
     4081 atcaaaggct gtctttgtaa ctctggttaa tgtgtgttta ttcatttaaa aatcctctgt
     4141 aactaaatag gggtcaaact cgaaatctgg ttgctcgttt tccattggct caatattccc
     4201 aacggcaata tgatgttcaa caatgccaat taatttcgcg cacatcacag caagcaaagc
     4261 gcaatattta tcgcttggta attcgtaact aagtctggtt gcctgtcgct gaagatgatt
     4321 tgtcacggtg gatgcgttaa tttcgtcaag attctttagc agataaaata gttttttgtt
     4381 gtatttcggg tggccttcac ttctacagaa cccaataaaa acatcatcaa gcttgcttgg
     4441 taactgctcg taactgtcaa agttagccag ataattttta atagtattct ttgttgtttt
     4501 gcgtactggc ataaatctgt ctcgtggcgt aatgctacga atgaccgctt caatgttctt
     4561 acgttcggat ttgctaaagg ttgggacttc gtagctggtg gggttgtttg tgtaagcgta
     4621 ggagttgtgc ttgttgcgtt ctcttgatgc ctgaagcatc gctctaaatt ctgaatactg
     4681 cattcatgtt ctgacatctc ataagatgtt attattctgt taacctatat atacaatatt
     4741 agcattttca aagactaatg tcaagtattt ttaacatttt cgtaacatct gtggtataat
     4801 gggtttatgg ggagagtaaa atatgaggcg tggctatgta tttggtcgca tattttgcat
     4861 tgcacacaga cctcttcaat cgccctgtaa cgagttaaac gacctcttca ggtcaactca
     4921 taaggtcagg tactttttgt gtcagaatgc gattgagagc gtcttacaag gaaaactaat
     4981 ggtaaatctc cctcctgagc tggggtcacc tgatgtatgg actggtggag gtattgggct
     5041 gattgcaggc ctgctacttc gctacgtcct cgtcagagtg cttgttgtct ttaaactgtt
     5101 cggtcgtaca tggacgcttg accttaagga taacgaccac acagaccccc gttaaactcc
     5161 ttcaaaaccc ctgcacgaga tgacagatag aagtctatgg tacaggggtg tctttagcat
     5221 tttgtgctat ctattaaaaa atagcttgac agaaccggaa agctgtatct atcaagcatt
     5281 cacctctctg acagtaattt ctaaaagttc ttatttttca ataagttgta attagccata
     5341 tatagagaac tgacaagtct cttcacagac aactctgcaa ccttaacagt aacttctaaa
     5401 aatgcttatt tttcaacgag ttgtaatcac ccaatatata aggctgtcca gtaccttcac
     5461 agataacttc aagccctctt aaccgcccca ctaatcggga gaagagcgat aaccccagag
     5521 agggaagctc ctttcttaac cgacctctta aacgaccttt acagaccttg ttcaaggtaa
     5581 tcacaatagt ttttcttccc tcggtttcct gtgttcctcg gctggctatt gagatttgaa
     5641 agacctctgt aagcgctctc ttagcgagag aaaaatcatt catagtgacc ccaagcgaag
     5701 cgcccctcta aaaaaagatt ttttagctat tttgtttaaa agcatatcaa acgatacatt
     5761 ttattgattt aggtcaaaga caaaagcatt gacaaaatgg taagattgtt cacatgacct
     5821 tacttattta agagactttg aaacagcctg tttaacaggt cactacctct ggtcttatcg
     5881 ttccct
//
LOCUS       DAZPLI010000211         5846 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZPLI010000211 DAZPLI010000000
VERSION     DAZPLI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.213"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(38..400)
                     /locus_tag="ACHUU3_003939"
     CDS             complement(38..400)
                     /locus_tag="ACHUU3_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR2083888.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            937..2850
                     /gene="mtlA"
                     /locus_tag="ACHUU3_003940"
     CDS             937..2850
                     /gene="mtlA"
                     /locus_tag="ACHUU3_003940"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR2083889.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3080..4228
                     /gene="mtlD"
                     /locus_tag="ACHUU3_003941"
     CDS             3080..4228
                     /gene="mtlD"
                     /locus_tag="ACHUU3_003941"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR2083890.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4228..4815
                     /gene="mtlR"
                     /locus_tag="ACHUU3_003942"
     CDS             4228..4815
                     /gene="mtlR"
                     /locus_tag="ACHUU3_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR2083891.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4827..5036)
                     /gene="yibT"
                     /locus_tag="ACHUU3_003943"
     CDS             complement(4827..5036)
                     /gene="yibT"
                     /locus_tag="ACHUU3_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR2083892.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5321..5683
                     /locus_tag="ACHUU3_003944"
     CDS             5321..5683
                     /locus_tag="ACHUU3_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR2083893.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1473 a         1445 c         1518 g         1410 t
ORIGIN      
        1 agcaccacgt aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat
       61 agtcagtctt tttctcggcg gcgagttgat gctctagccg ggaaattcgg tcagaaagtg
      121 cggcgatttc cgggtcagtc gctttctcct gcgacgcaac atgtgactgc atgccccacc
      181 cgcgctccgg ttgatagagc gtcgcccaga tccacaggaa cggccagata acatgcaggg
      241 taaacaggct cacccagccc gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt
      301 tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga
      361 cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt
      421 tatcagtgtt gtttttgggc tggcagccag aagggagtca ggctgatatt ttgacaataa
      481 tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct
      541 tagttacata acaattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg
      601 ccgtaagaaa atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa
      661 tgtgacacta ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt
      721 tacacaagcg ttttgtgatg aacgtcacgt taattacctc tctaccccct atatttatgt
      781 gattgatatc acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg
      841 cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca
      901 gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa
      961 gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc
     1021 gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg
     1081 gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt
     1141 ggtaagctgg taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt
     1201 atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc
     1261 tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg
     1321 ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc
     1381 ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg
     1441 gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc
     1501 ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa
     1561 ctgggtaaat caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg
     1621 ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc
     1681 atccacttcc tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt
     1741 ctgatcctcg cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc
     1801 ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca
     1861 aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt
     1921 gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca
     1981 actcgtcgta tgcaggacat gaaagctgag tctaaaggca catctccgct gtctgcgggc
     2041 gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg
     2101 ggttccagtg cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca
     2161 cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc
     2221 actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcgca gcatatttcg
     2281 ctgaccaact tcctcgacag cggcctgtac accagcctaa ccgaacgtct ggttgctgcc
     2341 cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat
     2401 tccagtgcta acctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagcagca
     2461 accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag
     2521 ccggaatacg ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag
     2581 tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg
     2641 gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt
     2701 ctggtgattg gtattgcagc ccgtaacaac gagcacattc aggttatcac cagcctgacc
     2761 aatgcactgg atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa
     2821 gtgctggaac tgttggcagg tcgtaagtaa tccaatccca ccctctccac atggagaagg
     2881 tggggttaat tgcctgatgc gctacgctta tcaggcctac aggatgcatc acgatttgtt
     2941 gaatttgcac gttcttgtag gccggataag gcgcttacgc cgcatccggc gctgcccctc
     3001 tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac
     3061 attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg
     3121 ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgccg atgtcaatca
     3181 ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg ttggtgaaac
     3241 cgagcaggta gataccgttt ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt
     3301 tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggcccggttg tgctggaacg
     3361 tattgctccg gctatcgcca aagggctggt gaaacgcaaa gaacaaggta atgaatcccc
     3421 gctgaacatc atcgcctgtg aaaacatggt acgcggtacc acgcagctga aaggccatgt
     3481 gatgaacgcc ctgccggaag acgccaaagc gtgggtagaa gaacacgttg gctttgtcga
     3541 ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc cgctggaagt
     3601 gacggtagaa actttcagcg aatggattgt cgataaaacg cagttcaaag gcgcactgcc
     3661 gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt
     3721 caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac
     3781 cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga
     3841 aagtggtgcg gtactgatca agcgctacgg ctttgacgca gacaaacatg cggcgtacat
     3901 ccagaaaatc ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg
     3961 ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccat tgctcggtac
     4021 gctggaatac agccttccgc acaagaatct gattcagggg attgctggtg caatgcactt
     4081 ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc
     4141 gcaggcggcg ctggcacaga tttccgatct tgatgccaac agcgaggttg tatccgaggc
     4201 ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac cctgcgcccg
     4261 aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt
     4321 gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag
     4381 cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg
     4441 gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga tctttctgtg
     4501 cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggag
     4561 ctgctgatgg cattgcgtga agaactaaat cacgacggca acgagtacgc ctttaccgac
     4621 gacgagatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc accgccgcca
     4681 cagtttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct
     4741 gtgcgatcaa caatggtcct ttcactgact gagctgattt ccagaatcag cttaaaaaaa
     4801 gcctttcaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg ccgataaagc
     4861 cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg tattccggac
     4921 ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg gcttgatgcc
     4981 atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg
     5041 atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt taggttcccg
     5101 gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccgct gctgtttatt
     5161 atcttttttc tatagatttc ttaattaatc aataaattgc gtagttttta agcatgttaa
     5221 tgattacgtt acagaactat tgcaatgtca tcagttgtga acaaagcacc tggtcgcgca
     5281 tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa
     5341 cgaaatcaaa cgtctcagcg atcgtctgga cgccatccgc caccagcagg ccgatctgtc
     5401 gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc
     5461 agaaattgct cgcctgcgtg aagttcatag ccaaaaactg agtaaagaag cacagaagct
     5521 gatgaagatg ccgtttcagc gcgcaattac caaaaaagag caggctgata tgggcaagct
     5581 gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg gccgtgaaat
     5641 gggcctgcag gagatgactg gattctctaa gactgcgttt taagcgcctc agacgactgg
     5701 ttcgatcata tcaacccgca tctggcttgc cttagctggt caatgcgggg actctcctgt
     5761 ctactcgcct aattaccaaa caattcactc cctttacgtc gatttaccgc tggttgatat
     5821 tctgaaaatg catttttacc gaaata
//
LOCUS       DAZPLI010000212         5772 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZPLI010000212 DAZPLI010000000
VERSION     DAZPLI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.214"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..661)
                     /locus_tag="ACHUU3_003945"
     CDS             complement(<1..661)
                     /locus_tag="ACHUU3_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR2083894.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     IL"
     gene            761..1351
                     /locus_tag="ACHUU3_003946"
     CDS             761..1351
                     /locus_tag="ACHUU3_003946"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFR2083895.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPVWWYSFPAMLKGYI
                     DRVWNNGLAYGDGQKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(1433..2173)
                     /gene="pflA"
                     /locus_tag="ACHUU3_003947"
     CDS             complement(1433..2173)
                     /gene="pflA"
                     /locus_tag="ACHUU3_003947"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR2083896.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(2365..4647)
                     /gene="pflB"
                     /locus_tag="ACHUU3_003948"
     CDS             complement(2365..4647)
                     /gene="pflB"
                     /locus_tag="ACHUU3_003948"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR2083897.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(4702..5559)
                     /gene="focA"
                     /locus_tag="ACHUU3_003949"
     CDS             complement(4702..5559)
                     /gene="focA"
                     /locus_tag="ACHUU3_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR2083898.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         1501 a         1349 c         1424 g         1498 t
ORIGIN      
        1 ccagaatgat attccctgcg acagcaattt cctgctcctt tgcaaattgc cagtggaaag
       61 gtttgccgct gggataacga aatacaacac attgatgatt aagcagatca tgaggatggc
      121 ggggtttgcc atatcttgca aagtactctg gcgttgctgc tacacataat ttcactggtg
      181 ggccgatcgc cacgctgatc atatcttttt cgacgatgcc actaagtcga acgccagcgt
      241 caaagccctg ttggacgata tcgacaagag agtcgtcagt tgtgagctcg actttaatat
      301 caggatattc acgtgtgaaa ccaaccagta aaggcattaa aaatatacgt gcagctaccc
      361 gggcggcatt gattttgagc gttcctgttg gtgtcagacg aaaatcattc atctcatcta
      421 acatgatctg aatttcgtcg aaagctgggc gcaatcgttc atagagatta gaacccgctt
      481 ccgttaacga tacgcttcgg gttgtccgat tgaataagcg aattttaaga cgttgttcca
      541 gtgttttaat actatggcta atggcggacg aggataagcc taactcatcg cccgctgcac
      601 gaaagctttg attacgggcc acagcaaaga aagtggcaaa gtcagacata ttcatccgca
      661 ttagtgaaaa tccttcatta actcatcctg aatcatcacg tttacatgat tatgccgcct
      721 gcgtaaagtg cgctccagaa cttaacgtgg aggtaaaatt atgcagtctg aacgtattta
      781 tttggtatgg gcccatcctc gtcatgattc attgaccgca catattgctg atgcgatcca
      841 tcagcgggca atggagcgga aaatacaggt gacggaactc gatttatatc ggcgtaattt
      901 caacccggtg atgacgccgg aagatgaacc agactggaag aatatggata aacgttattc
      961 tccagaggtt catcagcttt attcagagct gcttgaacat gacacgttag tggtggtttt
     1021 tcctgtctgg tggtacagct tcccggcaat gctaaaagga tatattgaca gagtatggaa
     1081 taatgggctg gcttatggag atgggcagaa attaccattc aataaagttc gttgggtggc
     1141 gctggttgga ggagacaaag aatcatttgt ccagatgggc tgggaaaaaa atataagcga
     1201 ttatttaaaa aatatgtgca gttatcttgg tattgaagat gccgatgtca ctttcttgtg
     1261 taatacagtg gtattcgatg gggaagaact tcacgcgagc tattatcagt cgttattatc
     1321 tcaggtacgg gatatggtag atgcactata agatgtgtta aaaacgctgt agcagaatga
     1381 agtgcggaat aaaaaagcgg caactcaata aagttgccgc tttacgggga aattagaaca
     1441 ttaccttatg accgtactgc tcaagaatgc ctttcacgcg ttccatggtc tctttcttcg
     1501 gtggtttaac accgtcgagt ttgtactctt cacccattgc cacccatttg tgtttgccca
     1561 gctcgtggta ggggagaagc tcgattttct caacgttgcc catatcacgg gtaaattcac
     1621 cgaggcgatg cgctgaatcg tcatcgtcag accagcctgg gacaacaacg tagcggatcc
     1681 acaccttcac atttttgttc gccagatatt tggcgaactc cagcgtgcgg tgattggaaa
     1741 ctccaaccag attttggtgg atctcgtcgt tcatctgttt gagatcgagc attaccaggt
     1801 cggttacttc cagcagttca tcaatcaccg gatcgtaacg acgaacaaaa ccgttggtgt
     1861 ccagacaggt atgaatgcct tcttttttgc aggcgcggaa ccagtcacga acaaactcag
     1921 cttgtaggat tgcctcaccg ccggatgcgg taacgccgcc gccggaagcg ttcataaagt
     1981 ggcgataggt caccacctct ttcattaaat cttcaacggt aacttcttta ccgccatgcg
     2041 tatcccaggt gtcgcggtta tgacaataca ggcagcgcat caggcagccc tggaaaaagg
     2101 tgataaagcg aatacccggg ccgtctacgg ttccacagga ttcaaaggag tgaatgcgac
     2161 caataactga cattgcggtg tttctccaga tgtggcccat ctgaggccgt gttggtgcgc
     2221 agctcgaagg ctacgtcgag tctgttttgg cagtcacctt agagtataga tagctgacaa
     2281 aaaaggctct cgtgctaaaa aaggccccac tttcgtggag cctttattgt acgcttttta
     2341 ctgtacgatt tcagtcaaat ctaattacat agattgagtg aaggtacgag taataacgtc
     2401 ctgctgctgt tctttagtca gcgagttgaa acgtactgcg tagccagata cacggatggt
     2461 cagctgcgga tatttttccg ggttttccat cgcgtcgagc agcatttcac ggttcatcac
     2521 gttaacgttc aggtgctgac caccttcgat ggatgcttcg tggtggaagt aaccatccat
     2581 cagaccagcc aggttggtct tacgaacttc gtcgtcttta cccagtgcgt tcggaacgat
     2641 agagaaggtg taggagatac catctttagc gtaagcaaac ggcagtttag caacggaagt
     2701 cagagaggct actgcacctt tctggtcacg accgtgcatc gggttagcac ccggtccgaa
     2761 cggcgcgcca gcacgacgac cgtctggggt gttacccgtt ttcttaccat acacaacgtt
     2821 agaagtgatg gtcagaacag actgagtcgg gatagcgtca cggtaggtgt gcagtttctg
     2881 aattttcttc atgaaacgtt ctaccaggtc aacagccagg tcatctacac gcggatcgtt
     2941 gttaccaaac tgcgggtatt cgccttcgat ttcgaagtcg atagccagac cgtcttcgtc
     3001 acgaatcggt ttaactttcg catatttgat tgcagacagg gagtcagcag caacggacag
     3061 accagcgata ccacacgcca tggtgcggat aacgtcacgg tcgtgcagcg ccatcagaga
     3121 ggcttcgtag ctgtacttgt cgtgcatgta gtggatgatg tttagtgcag tgatgtactg
     3181 tttagccagc cagtccatga agtgatccat gcgctccatc acttcatcat agttcaggac
     3241 atcgcctttg atcggttcag acttcggacc aacctgcatt ttcagttttt cgtcaacgcc
     3301 gccgttgatt gcgtacagca tggttttcgc caggtttgca cgcgcaccga agaactgcat
     3361 ttgtttacca acgatcatcg ggcttacgca gcaagcaata gcgtagtcat cgttgttgaa
     3421 gtccggacgc atcaggtcat cgttctcata ctgcagagaa gaggtgtcga tggacacttt
     3481 agcggcgaat ttcttgaagt tcagcggcag tttttcagac cacagaatgg tcatgttcgg
     3541 ttccggagac ggacccatgg tgtacagggt gttcaggaaa cggaagctgt ttttggtaac
     3601 cagggtacga ccgtcgaggc ccataccacc gatagattcg gttgcccaga tcgggtcgcc
     3661 agagaacagt tcatcgtatt ccggagtacg caggaagcga accatacgca gtttcatgac
     3721 caggtggtca accatttcct gcgcttcttg ttcggtgatc ttgccagctt tcaggtcacg
     3781 ttcgatgtac acatccagga aggtggaggt acgaccgaag gacattgcag caccgttctg
     3841 agacttaaca gcagccaggt agccgaagta agtccactgg atagcttcct gagcgttggt
     3901 agccggacca gagatgtcgt agccgtattt cgcagccatt tctttcatct gacccagagc
     3961 gcggtgctgt tcagcgattt cttcgcgcag acggatagtc tgttccaagt ttacgccgtt
     4021 ttccagatca gcctgcagag aagtgaactg tgccagtttg tctttcatca ggtagtcgat
     4081 accgtacagc gcaacgcgac ggtagtcacc gatgatacgg ccacggccat atgcatctgg
     4141 cagaccggtc agaacaccag atttacggca acgcaggatg tccggagtgt aaacgtcgaa
     4201 cacgccctgg ttgtgagttt tacggtattc agtgaagatt tttttgatca tcggatccag
     4261 ttcgcggttg tacgctttgc aggaaccttc gatcattttg ataccaccga acgggataag
     4321 agcacgtttc agcggagctt cagtctgcag accaacgatt ttctcaagct gcttgttgat
     4381 gtagccagcg tcgtgagagg tgatggtgga agcaacagcg gtgtcaaagt caactggcgc
     4441 gtgagtgcgg ttttccagtt taacgccttc cattactttg tcccacaggg tggtggtcgc
     4501 ttcagtagcg ccagccagga aggactcgtc accctcgtac ggagtgtagt ttttctgaat
     4561 gaagtcacgg acgtttactt cattctgcca gtcacctttg gtaaaacctt cccaggctgt
     4621 ggctaacttt tcattaagct cggacatgta acacctacct tcttaagtgg attttttatt
     4681 tactgcgtac ttcgacaacc attaatggtg gtcgttttca cgcaggtaaa tgacccagta
     4741 tgtcaaccca accaacaaac caccaccgat aatgttgccg atcgtaaccg gaatcaggtt
     4801 atcagtgatg aaattcatca cggtcaggtg agaaaaattt tccggtgcag aaccgactgc
     4861 ggtccaaaat tccggggatg cgaagtcgcg gattacaata cccatcggga tcataaacat
     4921 gtttgcgata ctgtgctcaa aaccgctggc aacaaacatc gcgaccggca gcaccataat
     4981 gaacgctttg tccatcaggc tgcggccaga ataactcatc catactgcca gacataccat
     5041 caggtttgcc aggataccaa gacagacggc ctcaataaaa gtatggtgca ctttgtggtc
     5101 ggcggtttgt aggacgttta gtccccattg accatttgcg gtcatatact cgccggaaag
     5161 ccacattaaa agtacaaaca gcagtgcgcc gaccaggttg ccaaaataga catttagcca
     5221 gtttttcgcc aactgacccc aggtgatgcg cccactcgcc ttagcaacaa caatcaacac
     5281 ggtggaagta aagagatcgg ctccgcagac aacacaaaga atcagcccca gagagaagca
     5341 aatgccgcca accagttttg ccatgccgaa gggcattgtg cctgtgccag tggttgctgt
     5401 gatatagaag acgaatgcga ttgagatgaa aacaccggcg gtaatcgcca gatagaaagt
     5461 cttaagcgga tgtttcgttg ctttatagac acccgcctct tcggccactt tggccattgc
     5521 agcaggaagt aaaagatcaa aagggttgtc agctttcaca ctaactctct ctttattaag
     5581 tcggcgacga gatactaaca aagcattata gatgagaaat tgatatagat catatctcgc
     5641 ctggcttata ggcccgtaac tcgcatggtt tttatgcaga tacggagtaa atatttgatt
     5701 atccaaataa aaataaattt taaaaattaa caaatgagtt gaattttttc cgcatcctcc
     5761 gctaaaacag tt
//
LOCUS       DAZPLI010000213         5640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZPLI010000213 DAZPLI010000000
VERSION     DAZPLI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.215"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHUU3_003950"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHUU3_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR2083899.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="ACHUU3_003951"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="ACHUU3_003951"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR2083900.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHUU3_003952"
     CDS             1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHUU3_003952"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR2083901.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="ACHUU3_003953"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="ACHUU3_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR2083902.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1401 a         1352 c         1529 g         1358 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat
//
LOCUS       DAZPLI010000214         5620 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZPLI010000214 DAZPLI010000000
VERSION     DAZPLI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.216"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..513)
                     /locus_tag="ACHUU3_003954"
     CDS             complement(<1..513)
                     /locus_tag="ACHUU3_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR2083903.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="ACHUU3_003955"
     CDS             complement(759..1211)
                     /locus_tag="ACHUU3_003955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR2083904.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHUU3_003956"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHUU3_003956"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR2083905.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHUU3_003957"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHUU3_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR2083906.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="ACHUU3_003958"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="ACHUU3_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHUU3_003959"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHUU3_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR2083907.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5494..>5620)
                     /locus_tag="ACHUU3_003960"
     CDS             complement(5494..>5620)
                     /locus_tag="ACHUU3_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR2083908.1"
                     /translation="HIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1339 a         1468 c         1460 g         1353 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa
     5461 aggtcggcaa tgaggtgatg gaacagggca agattacgaa agcccgctcc cctcaaggac
     5521 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     5581 gaatacctta tcattcgtca aaatattaat agcgatgtgc
//
LOCUS       DAZPLI010000215         5614 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZPLI010000215 DAZPLI010000000
VERSION     DAZPLI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.217"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..428
                     /gene="soxR"
                     /locus_tag="ACHUU3_003961"
     CDS             <1..428
                     /gene="soxR"
                     /locus_tag="ACHUU3_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR2083909.1"
                     /translation="PGEVAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYV
                     AIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDE
                     LDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="ACHUU3_003962"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="ACHUU3_003962"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR2083910.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="ACHUU3_003963"
     CDS             2475..4124
                     /locus_tag="ACHUU3_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR2083911.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..5569)
                     /locus_tag="ACHUU3_003964"
                     /pseudo
     CDS             complement(4278..5569)
                     /locus_tag="ACHUU3_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1255 a         1271 c         1457 g         1631 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc gctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt cgagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac cagggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgtacgccat ataaaaattt gaatagtcaa gaaaggtatt tttaatattg
     4861 gctctattca ttgctgcatt gtagaattga acatgcataa cggagcatgc atttagagtt
     4921 ccgtcggcca tttttatctg agcgaaattt gagtcatcga gaatacaatt atcaaaaagt
     4981 atttttccat tatggcgtaa taaaaattcg ttttatacat attacagtcc tggaaaatag
     5041 cgttttgtag tctgcaacct gcaaaattcg aattggataa atcttcatct ttaatcacga
     5101 tacctttcag gctaaggtgt gaaaaatcgt aaccaccatt tactgtctgt ggaatttcga
     5161 tagtattatt atggacggcg acattattta taaaatatac caccttaatg gatacttcct
     5221 tttctttact aaggtaaact cttctcgtaa ttctatcgtc acatttatta tttgcatata
     5281 aagctatatc tataaaatgt ttatgtattt tcctgtccgt ttccttcgtg atttttcctg
     5341 atggtaaact atcataaatt cttttcattt tctgaatcaa acctatcact ttttcatgat
     5401 catccatctt gaaaagagag agggctttat tgtcaattaa cgtgttttta tgatgtttgc
     5461 tgtaattata tgattttgtt ttctttgtat gtgatgaact tgtgaaagag tggttatcgt
     5521 taatctggca aagagagaaa aacatattat ttaaaccatt atagcgcata aaatatcctt
     5581 ataattaaca agaaagaaaa ggcatattct ctgc
//
LOCUS       DAZPLI010000216         5613 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZPLI010000216 DAZPLI010000000
VERSION     DAZPLI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.218"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(104..418)
                     /locus_tag="ACHUU3_003965"
     CDS             complement(104..418)
                     /locus_tag="ACHUU3_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFR2083912.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
     gene            complement(423..638)
                     /locus_tag="ACHUU3_003966"
     CDS             complement(423..638)
                     /locus_tag="ACHUU3_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR2083913.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(789..2642)
                     /locus_tag="ACHUU3_003967"
     CDS             complement(789..2642)
                     /locus_tag="ACHUU3_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFR2083914.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCCGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            3397..3513
                     /locus_tag="ACHUU3_003968"
                     /pseudo
     CDS             3397..3513
                     /locus_tag="ACHUU3_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3618..3694)
                     /locus_tag="ACHUU3_003969"
     tRNA            complement(3618..3694)
                     /locus_tag="ACHUU3_003969"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3658..3660),aa:Arg,seq:tct)
     gene            complement(3708..3784)
                     /locus_tag="ACHUU3_003970"
     tRNA            complement(3708..3784)
                     /locus_tag="ACHUU3_003970"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3748..3750),aa:Arg,seq:tcg)
     gene            complement(3792..3867)
                     /locus_tag="ACHUU3_003971"
     tRNA            complement(3792..3867)
                     /locus_tag="ACHUU3_003971"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3832..3834),aa:Ile,seq:cat)
     gene            complement(3908..4966)
                     /locus_tag="ACHUU3_003972"
     CDS             complement(3908..4966)
                     /locus_tag="ACHUU3_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR2083915.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(5074..5314)
                     /locus_tag="ACHUU3_003973"
                     /pseudo
     CDS             complement(5074..5314)
                     /locus_tag="ACHUU3_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5539..>5613)
                     /locus_tag="ACHUU3_003974"
     CDS             complement(5539..>5613)
                     /locus_tag="ACHUU3_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR2083916.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT         1339 a         1583 c         1320 g         1371 t
ORIGIN      
        1 cagcgccaga acagcagctg acaggccgta tttgattttt gcgctcatgg atatttatca
       61 ggatgctacc aatgaaagat actggaaagc caactgcaaa aagctaacaa cccgtaatcg
      121 agttatcaga actgttaatt tttatggtat accgcgcctc tgaacagggg cgcgtttctg
      181 gcaacagctc gtccccttca cataacccgg cagcaacatc caagaagacc tgtctgatgc
      241 tccttctggc tgctgcctca taaaactcca gcgcggcacc ttcaacacgg tccagcgaga
      301 tgtccaggtc aaaaatttca ccgtcaaagc gttttttgtc ccgtaacgct aaagttaccg
      361 taactttatt ctcaaaattg cggatccctt tcacaatcag ttcatagttt tgagtcattg
      421 aattactctc cccgtgcagc cttacgacgg tcctctctga ttttgaaata caggttagtc
      481 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
      541 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      601 tatgacacac ctgttgtgat tttttccatc tggtacatac cccgtctccc gttatccgga
      661 agctgacaac aataaaaaaa gccaccagtt aagtactgat ggctctgata actcatgcag
      721 gcatctcaga cgacccactg acactaccgg tgagtttaac gataccttcc atttggctgg
      781 ctcacttttt atgatgatgc cggtgcattt atctccagca ccagactttc tatctcaacg
      841 ccatacgctg catttttggt aatatccgtc agcgtcagcg cattcagccc cagtgtcaga
      901 ctgtctttta tgacctggaa tgccgggcca gccactccat tcagtttcgg agtaaccgtg
      961 gcactgccgg cggtgaacac cagctccagc gtcagccagt cgttactgta attcccgaac
     1021 tcgcccaact ttgtgtttcc tgctttcttg tgatgcatca gattcagttt gccgtctgtg
     1081 gtctgggtga agaacgacat caggaacggg ttaccagtcc cggtcatcgc cacgacgtca
     1141 ggtaacgcta catcggtata cagataaatt cccagaccga actggttgtt ggtcagtgcg
     1201 cctgacagtc gaaacttaca gctcagtctg ccaccccgtg tcagcaggga gactgcgtca
     1261 tccaccgggc gcgtcaggga ccaggcttta ttgctctgct tggtgatctt aaatacacca
     1321 tctgacaact gaactccgcc attcttaatg ctccagccct gcgcagcagc gtctccggct
     1381 gtcggcaaca gggagattgt gcgtatggat gcatcttcag acggccccga tggcgtgtcg
     1441 ccgccgggcg agggtttgat ttccggtgcc ttaccactaa tgaaggctaa ggtgcgaccg
     1501 gctacgttca gaatagcagt tgccatacga tcgggaataa tgccacgacg cgcccatgag
     1561 ctgaaatgcg tcgggcgatt tgatgatacc cagtttttgt tcgttcggga tgccgaaccg
     1621 taataaccag acccggcaat atcaggatct tctgacgggt tgtttgtcgg tgtattaact
     1681 ccactaccat cggtcataaa gggaacaaaa taaatctgct gggattcttt acctttatat
     1741 gcaccatata ccacttcata ttgcgtaccg tgttcttgtt tccacgcgta tgtcgtgtcg
     1801 ccacaaatcc aggggactga tgccggactt ccaccgtgac actgcgccgc cagcccggca
     1861 aggtcagcac ggaactgctg taccattgca agaaatgctg ctggctgctg ggcgtaactg
     1921 gcattcgtca tatcgaattc cccctgcatc cagcatatcg ccagcaaaac gtttttcggg
     1981 tttttctgca atgctgcctt cgtgcggaaa agcagatcct gatataacgg cttacccact
     2041 ccccagcgag ccgaatcctg actggccccc gtggactcgc tgaatgtccc ctccgtgccc
     2101 tgggtgaatg ccgaaccacc acaacagcat ggtaccagca ggatccccgc attattaggg
     2161 atatacggaa gcagtttttt ggcaatatgt aagccctgtc cgacacagcc gtactgccct
     2221 ttgctcaggt cagcccgggg atggttaatc gtactcatat cctgaacatc atgcagacaa
     2281 tggtcagcag gaatgatgtc gttaaatacg catacttcac caccgggagt cactgtgtta
     2341 cgacgggcca gttgcttaat gcgcggatgg ggcgcatcgt atgaatccgg aagcggaaga
     2401 ccttcaccgt aagccatggc attggattgc ccggccagta cgatgacgta gtaccactcc
     2461 ggctcagttg caccactgac gaccacatca ccttctgctg caatcgcctg catcagggta
     2521 taaggggtta tggccaccgg actaccaaac ggctgccagc cctccttcag tttttgtgtc
     2581 agtcgtttcg caaggtctga cggcgatgcc gccctgacca cgtcatagtg tttaaatgcc
     2641 atgaatcctc ccggccggga taatattgtg agtaaaataa ggagcgggct gaagtccgga
     2701 agttacagga caatggcaga agagagacga cagcccgcaa ttcgaaaaag accgcgcagt
     2761 tgcgcagagt gattactatg gggtattatt cgccagctga aatattactt cacgttttat
     2821 tgtttattcc ttgccgcccg cgtctcccag cgcgggcttt ttttgcccac aagaaaaccc
     2881 ctccggagag gggctaaagc cgcgtatctg tatcatcatg cacatggtgc cgggtgcctc
     2941 ccggtgagtt cagcccggtg ccactaaacc cgcgtcattc tcgttttgat aatcagagat
     3001 tataccgtca ccagtcgccc ctccgctcag ggggattcac catgcgaaat ttttttaaca
     3061 aatgcccagt ctgacaggca actgtcaact tactgaattg tgagcaacat agcatttaac
     3121 ggggaacctg ttttctgcag taaaaaggcc caccggagcg gatgggcctg gaaggatagc
     3181 ggtcatgtga tgccggtttc ccggtaactc agcaccggta tctgagtcaa cgttttctct
     3241 actgggtcat ttccgatacg ccctgcctgc tgacaggctt tcatcacatc tgaaaatata
     3301 gcaccctgac tgatactgta gtacctaagg ttccagaaac tgtgatgtat ccggcacaga
     3361 aaagcccctc cggagagggc tggagagtgg cgctatgtgc cattgcatga tgccgggtgc
     3421 ctcccggtga attcagtacc agcacctgaa tccgcgatta tcccatatac ctactcgctg
     3481 attgcccctc cgcacagggg gattcaccat gccagtttct tttaacaaac tccccgcaaa
     3541 acagacaact gtcaaccgtc tgaattgtga gacatttaaa aaaaaggccc gcaaaagcga
     3601 gccagggaaa ataagtgtgg cgcgttgtac tggattcgaa ccagtgaccg attgcttaga
     3661 aggcaattgc tctgtccggc tgagctaaca acgcatgatg cagataatgg accgccatcg
     3721 gggacttgaa ccccgcgcag ccagcttcga aggctggcgc tctttcctga tgagctaatg
     3781 gcggtatgtg atggtggccc ttgctggatt tgaaccagcg acctggcgat tatgagtcgc
     3841 tcgctctcac cactgagcta aagggccggg agccgcataa taacgacgcg taattaattc
     3901 ttcaatatca tccgttctgg ctgactaaat cctgtacttc ccgaaccgtc tgctcaaaac
     3961 gttcagtctc cagctcaacg ccaattgcac gacgcccgag cgccagtgcc gctttcactg
     4021 tcgaacccaa ccccatgaaa aaatctgcaa tcaggtcacc cggacgactg ctagcgctga
     4081 ttatctgctg cagcatttct gccggttttt cgcacggatg tttcccggga tagaactgca
     4141 ccggtttatg cgtccacaca tcggtatacg gcacctgcac cgtcacgcca aaataccgcc
     4201 gcagatgttt atattcactc agcagctccg catactgccg gttcagtgag gtatacgtat
     4261 ccaccagctg gtggtggagc ttttccagtt caccacgccg atgcttctct tctgccaccc
     4321 gggcaaacag cgcctgtaat ttcagataat cgctttcatt cggcaactgc cactgaccgg
     4381 cactgaacca gtgcgacacc atgtttttct ttcctgtggc atctgcaatc tgttttgccg
     4441 ttatccccag ggcagctcgc gcatcacgaa agtaagaaat cagcggggcc atcacatgct
     4501 gtttcagtgc actgcccttc gccgcatacc cggcatcttt cggacgatac ggcccctgat
     4561 aatgttccgc gaacagaatg cgctctgtgg cggggaaata cgcccgcagg ctttccttgt
     4621 tgcatccgtt ccagcgtccg gacggcttcg cccagataat atggttcagc acactgaagc
     4681 gttcacgcat catgatttcg atatcagatg ccaggcgatg accacagaac aggtaaagac
     4741 ttccggcagg tttcagcacc cgccagaact gcgccagaca ctggtccagc cacttcaggt
     4801 aatcatcgtc gcccttccac tggttatccc agccctcagg cttcacttta aagtacggcg
     4861 ggtccgtgac tatcaggtca acagaatttt cgggtaacga ccggataaat tccaggcagt
     4921 cggcgttgat taactcacaa ctggatattt ttacagtatt aagcatggat cattaagcct
     4981 gtctctgata ggctcattct gcttttgcgc aaagcagtgg gcctgaggtt tgcttgtgaa
     5041 cccaacgcat gagcagatgg ctggtgggtg cccctaacac ccaccagccg cccatttacc
     5101 acaaataaaa aagccttcac tgcggaaggc gtctgtaaca accgaactga tagtctgcca
     5161 gacccgccat aaccagctgg gtcagtatta actggcagcg ttcgcgtgaa aggtaagtat
     5221 tctgcgctat ctccccgact gtcgccggtt cggtaacgct taattcatta aacaccactc
     5281 tggcggtttc tgtcatatcc tgctgtttta gcatgtcttt tccttttccg gttaacgtga
     5341 cacaccaata actcttgtcg aaaaagccag caagctgaaa gacaggtatt caccgccacc
     5401 agcgcgttta ctgtactgac gcgatttcag tcataaaaaa cccgctcggc ggcgggttgt
     5461 agaaactctt ctaacgtcag gcataaaacg cccatcgtta tgacgaattt accacagatt
     5521 ccggaaaaat caaccttgtt acctagttac cttttttaac tgccgctcag cccatgcttc
     5581 ttcaatatca aaccgggtca ccagcgcatc ata
//
LOCUS       DAZPLI010000217         5541 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZPLI010000217 DAZPLI010000000
VERSION     DAZPLI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.219"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..1369)
                     /locus_tag="ACHUU3_003975"
     CDS             complement(<1..1369)
                     /locus_tag="ACHUU3_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFR2083917.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWL"
     gene            complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="ACHUU3_003976"
     CDS             complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="ACHUU3_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR2083918.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="ACHUU3_003977"
     CDS             complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="ACHUU3_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR2083919.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="ACHUU3_003978"
     CDS             complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="ACHUU3_003978"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR2083920.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="ACHUU3_003979"
     CDS             complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="ACHUU3_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR2083921.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         1410 a         1450 c         1304 g         1377 t
ORIGIN      
        1 atagccattg ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc
       61 agaaaagtaa agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg
      121 gcccgcttaa atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca
      181 agggaattgc taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga
      241 tttgcacggg cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg
      301 cacgcaatac cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca
      361 gtgccgcgac cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca
      421 tcagcaaaat tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac
      481 ttaacttaag cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca
      541 ttcccgtcaa ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc
      601 agtggatcca gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca
      661 acaccgcaaa agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc
      721 gcatgatatt tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa
      781 taaccgcatt ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac
      841 gcaggctaca attcggttca ataacacttg cctgcagaaa gcggccggtg agcggctgat
      901 gctgggcaat ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg
      961 cacgaacttt gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt
     1021 cgccatacag cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt
     1081 gaccgtaatg agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg
     1141 cgccagttaa ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga
     1201 aaaacagcaa cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc
     1261 aggcgaagag agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca
     1321 acggaaaaat tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc
     1381 tgtttatgga aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc
     1441 gaggcacatt cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag
     1501 cttccagttc ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca
     1561 agtttgagta aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt
     1621 aaagtgagga acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc
     1681 ggtacgtggt gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc
     1741 gccctgcgca agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt
     1801 cttggcggga atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat
     1861 gattcctccg gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc
     1921 ggccgtagcc gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact
     1981 caacaaactt cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt
     2041 gaaagcttca gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc
     2101 gattgcatct ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt
     2161 tttacgatca acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt
     2221 agcgtcttca acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc
     2281 agccagttct acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc
     2341 gcctttcttg ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc
     2401 cagggagata cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc
     2461 ttttttgtat tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg
     2521 aaccaggccg tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt
     2581 gattttacct tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt
     2641 agctttgcac tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac
     2701 cataacttcc actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt
     2761 ccaatccatt tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca
     2821 gccgtagtca gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt
     2881 agcgatagct acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg
     2941 ttcgcggtcg aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct
     3001 cggatgctta acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc
     3061 cagatcaacg aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc
     3121 ttcctgcagg ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc
     3181 acgacgagaa acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag
     3241 ctctttgcct tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg
     3301 caggaacgca cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat
     3361 aacaccggta acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg
     3421 acgtttagct ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag
     3481 agcaacgtca acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg
     3541 ctcagccggg atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc
     3601 gatagcaaca acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga
     3661 ctcttcaaag agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg
     3721 tccacctggc tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac
     3781 gggggtactg caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc
     3841 gctttttcaa tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggct
     3901 gccggaacca gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg
     3961 gccaaaaggc gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga
     4021 tgcgcacgtt cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc
     4081 accgttccca tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg
     4141 cgacgcaata atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc
     4201 acttcctgag tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg
     4261 ccattggttg acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct
     4321 gacgcaacat caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc
     4381 gaatccagca gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct
     4441 gcaccgcttg ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta
     4501 aaacaggcgt accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta
     4561 acgttgtgtg caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata
     4621 gcgggctggc agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg
     4681 atcacggatg tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt
     4741 gcgaccgcca tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat
     4801 gccacgtttc ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga
     4861 gtatttagca ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga
     4921 agccacaccg ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt
     4981 catttcagcc agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc
     5041 aatttcgttg tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc
     5101 catcgcccat gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt
     5161 aatattgtta cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt
     5221 acttgcgact tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc
     5281 atcggcatcg cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt
     5341 ggtatcaata ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc
     5401 cagcagtaat ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta
     5461 tgtatcaaca acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc
     5521 cgaaaaaaaa cgccgacaat g
//
LOCUS       DAZPLI010000218         5527 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZPLI010000218 DAZPLI010000000
VERSION     DAZPLI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.220"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="ACHUU3_003980"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="ACHUU3_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR2083922.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="ACHUU3_003981"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="ACHUU3_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFR2083923.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="ACHUU3_003982"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="ACHUU3_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR2083924.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHUU3_003983"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHUU3_003983"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR2083925.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHUU3_003984"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHUU3_003984"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR2083926.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..4812
                     /locus_tag="ACHUU3_003985"
     CDS             4447..4812
                     /locus_tag="ACHUU3_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR2083927.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4962..5480
                     /locus_tag="ACHUU3_003986"
     CDS             4962..5480
                     /locus_tag="ACHUU3_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR2083928.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT         1519 a         1391 c         1241 g         1376 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatgtttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaata
     4801 cgattaaatt aattaaaacc ccacaaataa attaaacata agattttact cattgtgttt
     4861 tatttctcac attgatgacg atcgcatgaa aaaatgatat tttaatgagc attctgaaaa
     4921 aatatcgatg catttcgagc gaagatggtg gggatccctg aatgcgtttt tctcaccgac
     4981 tgttcttact tctgattctc ctgctgacgg gagcaccaat actcgctcag gagccttctg
     5041 acgttgcgaa aaacgtgcgt atgatggttt ccggtattgt cagttacacc cgctggcctg
     5101 cgctatcagg gccaccaaag ttatgtatct tttcatcttc acgttttagt accgccctac
     5161 aagagaatgc cgcgacatct ttgccttatc tgcctgtcat cattcacact caacaagagg
     5221 cgatgatttc aggctgcaat ggtttttatt ttggcaacga gtcaccgaca ttccagatgg
     5281 aattaaccga acaatatccg tcaaaggcgt tattattaat tgccgaacaa aataccgagt
     5341 gcattattgg tagcgccttt tgtctgatca tccacaataa tgacgtcaga tttgccgtaa
     5401 acctggatgc cttatcgcgt agcggtgtaa aggtcaaccc ggatgtctta atgctcgcac
     5461 ggaagaaaaa tgatggataa cgataattct cttaataagc gccccacgtt taaaagagca
     5521 ttacgca
//
LOCUS       DAZPLI010000219         5527 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZPLI010000219 DAZPLI010000000
VERSION     DAZPLI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.221"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..115)
                     /locus_tag="ACHUU3_003987"
     CDS             complement(<1..115)
                     /locus_tag="ACHUU3_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR2083929.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLE"
     gene            complement(220..1722)
                     /gene="dtpA"
                     /locus_tag="ACHUU3_003988"
     CDS             complement(220..1722)
                     /gene="dtpA"
                     /locus_tag="ACHUU3_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR2083930.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(2334..2969)
                     /gene="nth"
                     /locus_tag="ACHUU3_003989"
     CDS             complement(2334..2969)
                     /gene="nth"
                     /locus_tag="ACHUU3_003989"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR2083931.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(2969..3664)
                     /gene="rsxE"
                     /locus_tag="ACHUU3_003990"
     CDS             complement(2969..3664)
                     /gene="rsxE"
                     /locus_tag="ACHUU3_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR2083932.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(3668..4288)
                     /gene="rsxG"
                     /locus_tag="ACHUU3_003991"
     CDS             complement(3668..4288)
                     /gene="rsxG"
                     /locus_tag="ACHUU3_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR2083933.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(4292..5350)
                     /gene="rsxD"
                     /locus_tag="ACHUU3_003992"
     CDS             complement(4292..5350)
                     /gene="rsxD"
                     /locus_tag="ACHUU3_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR2083934.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         1449 a         1401 c         1366 g         1311 t
ORIGIN      
        1 tttcgagacg ttttttcatt aaatccacac tgacgagggt aaaatccttt ccgctctcac
       61 gcagggtgat atgggaagcg agagagcagg cacccggttt gtagaacaat ttcatcggta
      121 actccttttg tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaagccgcta
      181 acgaaaagtt agcggcttgt aaaagagttt ccctgaattt tacgctacag cagctttcgc
      241 cgctttgtct gcagcgtcat cctgcgtcat acggtgcagt ttaggcgcgg tcagcagcat
      301 cagtactgca ataatggcag tagcgacacc aatctgcaag aatacgcgac cataaacttc
      361 cagtgacatc agcgggtcgg taacgttatc cggcacagcc atcatacccg caacataacc
      421 accaatcagg tttgcaccgg cagtggtcag gaaccagcta cccataatga agcccatcag
      481 acgctgcgga acgagttgtg caaccattgc cagacccaga ccagagatca tcagttcccc
      541 gatgctctgc aggccatagc ttgcgaccag ccagcttaca gacacgatac cagcgtcaga
      601 cgcgaatttc gcacccagcg gcagaatcag gaacgcacca gaacacatca ccatgccgat
      661 tgcaaacttg gttggcatcg gcagggtatc gcccatcttg ttatagatag cggccagaat
      721 cggactaccg atgatgatcc agaacgggtt cagtgcctga tactgctcag gttctacggc
      781 cagacccaga atggagtgct caacgttacg aatcgcaaag aagttcagtg acgttggcat
      841 ctggctgtac agcacgaaga agataatggc ttcgagcatc aggatgaagg caacgatcat
      901 tttacgacgc gcagcacctt tcatcgcgaa ggcttcttta ccgaagataa ccacgatacc
      961 gaaggcaaca acgcccagcg ccatacgcgc aacttcctga ttgtgcagca gccaggtggc
     1021 gatagcgatc agtgccacaa caccaataat ggtcagcagc aggttacggt agttgatagg
     1081 ctcgaagtct ggttttgaac cgtactgttt aacccagcgt tggcagaagg cgaagttaac
     1141 gatagtgatc agcaggccta caacgctcaa cgcaaacgca acactccagc cgtatttcgc
     1201 ggccagccac ggcgtagcaa tcatagagaa gaaagagccg atgttgacgg acatgtagta
     1261 catggtgaat gcaccgtcca gacgcgggtc gtttttctca tagcatgtag aaagcagaga
     1321 agatgggtta gctttaaaca ggccgttacc gaccgcaata gccgccatac ccatataaac
     1381 gataccggcg tcgtgaccag accaggcaac cagagcataa ccaatcgcca gcacaatagc
     1441 gccgagcata attacgcgtt tagtacccag taccttgtca cctaaccagc cgccgatagc
     1501 gaccagacca taaaccaggg cactaaagga agagaaaagg gtgattgaat ccgcttcaga
     1561 catacccagt tgtttaacca ggtaaacagc cataattcct tgtaggccgt aataaccaaa
     1621 acgttcccat aactcaatcg agaagatgag atagaacgcc ttcggttgtt tgaaagcgtt
     1681 caaactgacg ctttcagttg gtttttggtt tgcagtggac acttttacct ctttttttac
     1741 atcccatatt aatggggggg ctttctcgcg ttatgtctaa aaaacatacg cgcaattgtt
     1801 atagtgggag gggaaacggc aggtaatgtt cactatcttg cactttcaga caagtccttt
     1861 gtaataatct gttacatata actgaggcgg ggaattcaac cattccggga aatgttatcc
     1921 agcgttaaat ttttcaaaaa acgtaatggc agattttttc actgcactga gagggggggt
     1981 gatggcttaa cggcgatatg ttctgctatt tggcgttttt actagtgaga tagtccagtt
     2041 tctgaaaaat agccagtgta atgttttgta ggtcaatatt tgttgcgtgt gtaaatttaa
     2101 attattttaa cctgattctt gcaatgtttt tacattgcga cagcatcgtg atcttgatca
     2161 cgaatgaata gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc
     2221 aaacggatta cctgatgata acgctaagca atggcatcat tcacgttaaa aagatgtttt
     2281 aaaagaggat aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg
     2341 tcaactttct ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg
     2401 cgggcaatgc aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac
     2461 tctgccggaa ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca
     2521 aattgagtac gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg
     2581 aatgcagtgt ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc
     2641 gcagcacgat cttccggaac ctcgccatta tgctgctcca gtaagatacg gcaggttttg
     2701 atgatatttt ctgctttgct gttataaagc ccaatcgttt tgatataggt tttaacccct
     2761 tcaacgccca gttcaagcat cgctgcaggc gtattcgcca ccgggtaaag tttcgccgtc
     2821 gccttattaa cactgacatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca
     2881 aaaggcgaac tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg
     2941 aggatctcca ggcgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg
     3001 cacgttcggc agctgcttca gtacggcgct ttttcattct ttcatcaatc aggtattttc
     3061 ctgccagcat cagtcccagg ccaataaatg cacctggtgg cagcatcgcc agcaggaaag
     3121 gggagtcggt gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat
     3181 ctgcaccgtc aaacaatgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca
     3241 tggcgcaggt tgcgcccata ccaattgaaa agccgtccag tgccgaaagc gccggacctt
     3301 ttttggcggc gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa
     3361 taaaaatccc taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag
     3421 cgctgaccac cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc
     3481 agtgacgcag cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc
     3541 ctaaacccag agcgttagtg gcagtggacg tgaccgccaa cagaggacaa aggccgagca
     3601 actggaccag cgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc
     3661 tcacgcttta ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt
     3721 acaatccggc gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga
     3781 actggtcgaa atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga
     3841 tttttttacc cgcaaaatgg gtgatccagt cagaaaggcg cagttcgatt ttatcgccaa
     3901 gccctggcgt ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgccg ttaaaatcgg
     3961 ctcccaccag cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg
     4021 cggctaccgg tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact
     4081 ctggcgcagt taccagatag caactctgtg ccagcgtatt gttatagcgt tcggctggca
     4141 gcacctgatc aaataatgcc ttttgttgca gactggcctg ttcagcaatc gtcgttttgg
     4201 tcatctggtt gatggccgca gttaaccctg ttgaacccgc tgcaaatagc gctaacgtaa
     4261 tgccgtgttt tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg
     4321 gacgcgtgta gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca
     4381 cgccgtcagg atagccgccg aaactgcgga tcaaccagac taataagccc gcaagcgcgc
     4441 cgaaaataag acgaccacga ttggtcgtag aagcggtaac cgggtcagtc aaaataaaga
     4501 atgcgccgag catggtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg
     4561 gtgagaacaa ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa
     4621 tatgccagcg aatcgctttc tgccatagca accacaggcc gccagccagc caggcgagat
     4681 ttacccattg ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct
     4741 gttcaaccga atgaccggca cggacagagg ttttaaatgt atccagcggt gtcgcctgac
     4801 taatgccatc aatacctaag cgtagtgtgt tcatatcacc accactggcg gtatgcccgc
     4861 tgaaaataac ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg
     4921 gtaaccagct ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg
     4981 ccggattaaa cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa
     5041 acaccgtacc cagcacgacc atccaccatg gcgcgagggg gggaatactt accgccagca
     5101 ataagcctgt cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta
     5161 gtttgagtac gagagcttcg gctaacagag tactaaccga tgccaacagg atctgaacga
     5221 gagtacccca accaaaaaac cacagttgcg ctgcgattcc tggcacggct gcgagcaaca
     5281 ccaacagcat aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc
     5341 tgaataccat ttagtcctcg tttacaacct tctgctgggc ggcttttttg gcctgagcgc
     5401 gggcaatagc cgcggcaact gccgctttgc gcggatcaac ctgttcttct ggttctgcgt
     5461 tagtctgttg ctgttccagc ttgcgcgctt tggcacgggc aatagcggct tcgacggcgg
     5521 ctttgcg
//
LOCUS       DAZPLI010000220         5473 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZPLI010000220 DAZPLI010000000
VERSION     DAZPLI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.222"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..168)
                     /locus_tag="ACHUU3_003993"
     CDS             complement(<1..168)
                     /locus_tag="ACHUU3_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083935.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQ"
     gene            complement(312..1070)
                     /gene="aroD"
                     /locus_tag="ACHUU3_003994"
     CDS             complement(312..1070)
                     /gene="aroD"
                     /locus_tag="ACHUU3_003994"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR2083936.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(1101..1967)
                     /gene="ydiB"
                     /locus_tag="ACHUU3_003995"
     CDS             complement(1101..1967)
                     /gene="ydiB"
                     /locus_tag="ACHUU3_003995"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR2083937.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1979..3244)
                     /locus_tag="ACHUU3_003996"
     CDS             complement(1979..3244)
                     /locus_tag="ACHUU3_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2083938.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3471..4685)
                     /locus_tag="ACHUU3_003997"
     CDS             complement(3471..4685)
                     /locus_tag="ACHUU3_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2083939.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMGLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(4785..5141)
                     /locus_tag="ACHUU3_003998"
     CDS             complement(4785..5141)
                     /locus_tag="ACHUU3_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR2083940.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(5277..5384)
                     /gene="rprA"
                     /locus_tag="ACHUU3_003999"
     ncRNA           complement(5277..5384)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHUU3_003999"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT         1594 a         1250 c         1237 g         1392 t
ORIGIN      
        1 ctgtttatat ttatcagcaa gagctgtaat taacgtggtg gcttccagaa taccgccgcc
       61 agcgcctaac acacaaagtg ttgcttcgtc gggaatataa ttcaccgctt cctgtgccga
      121 caggaccggc actcgtccat taatacgagg tggttttaca ggtttcattg ttaattcctg
      181 attcagatgt gattttccct acgccagatg gagaattagc gcacagagac tcacgaaata
      241 tttttattaa atacggttaa atatattttt taagttcggc ggggagggtg ttcccgccga
      301 aatattattg cttatgcctg gtgtaaaata gttaataccg tgcgtaaatc atttaccgag
      361 atttgccctg gtgcagaggc tttttttacc gcaccaaaag ttgccgccga gccaaatact
      421 tcaccagcca gacgagaaat tacgccagtt tttgccatcg acatcgtgat gattggacga
      481 tcggcatact gctcctgcat ctccagggtc gcggtaagca acgtcagcac atcgctggta
      541 ctttgcggca tcagcgcaat cttaggaata tcggcgtcga aggactgcat tttgcgcaga
      601 cgggcaatga tttcttcggc ttccggcgtt ttatggaagt catggttgga catgacaact
      661 ttcacatcat gcgcgtgggc gtaggcgacg gtttctttaa cctgatcatc gccggtaaat
      721 aactccagat cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt gagagcaata
      781 taagcctcgg tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc
      841 agcggttttt ctggcatggt ttcacggaga atttttgccg ccgccatgac agactccaca
      901 ttggagaggt cggcaaagtg gtccacacgc cattccagaa tatcaaagtc cgcttcacga
      961 taggcgagag cttcggattt cacgcgggcg atatctttcg ccatcagcga gacgatgatt
     1021 ttaggtgcgc ccgcaccaat gacgagatct tttacagtta cggttttcat tttttaccct
     1081 ttctgcacgc ggtcagcctg tcaggcaccg aaccccatga cctgtttaac atattccaga
     1141 gggaaatctt tgccagtcca taacgtgaac tgttcagccc cttgccacaa caacatgccg
     1201 tatccatcaa tcgttttgca acctgcttgt tgtgcctgct gcaataactt cgtcatatgc
     1261 gggttataca cgcattcagt gaccagcagt cccggatgta acagactgat atcattaacc
     1321 aatgattcat tctcaagggg gttcatacct acttttgtgc cattggttaa aatgtcggcg
     1381 gaagccagga cttcagcaaa ggcttgctga tcggcgagat cggtaaccgt gacgacacaa
     1441 tcggtgtttt cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga
     1501 cggttaaaga gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc aatcgctgtt
     1561 gaggcacctc cggcccctaa cagcaccatc gttttgcctt tgatatcaaa accgctctct
     1621 ttaatggcgc gaatatggcc cgtgccgtcg gtgttatagc cacgcagata gccatcatca
     1681 ttaacgatgg tgttgatggc acccaccagt ttggctgctg gcgttaattc atcaacatat
     1741 tcacacgcca gttgtttgtt cggcatcgac acaccagttc cgcgcatttt gagggctttt
     1801 aatccttcaa ttgctccggg aaagctatcg ttatccactt cgaaggccat ataggtaaat
     1861 ggcaatcccg ctttttctaa ggctttattc tgcatttcag gcgataaact gtggcggata
     1921 ggataagcca tcaacccaat caattcgtat tttgcggtaa catccataat taactccttt
     1981 aacctctatg cttaattgtg tttaaccggg tgctaaattt gcgttcgcca aaccgcacat
     2041 cattctcagg aataataaaa acgcggtaat agcggctaaa aacaataatt gcggtaatca
     2101 gggccagcag cgcgaaagta aaatcgagaa caatgatata ttgcaggccg atgttcgaca
     2161 gataaccggt aatcagcgga ataacaaagt tagccagtcc acccatcatc atataaatac
     2221 tggtgacttt ggctttgctt ttggggaaaa actctgacat gaccgaaaca ccgagctgta
     2281 aaatgccgcc agctgctgag aaaccgataa caaaggcgcc ggcattacac accagtggag
     2341 aagggtacag gtagataatg gcagctgtta ttgttgccag cgcagagtta aatacattag
     2401 cccagatggg ccggaccatt tttttcagta gtgcggcaaa aataaagaca cagaccaacg
     2461 agcccatact gtaataagag atggttttta atgcctcagc ttctgacata ccagcaaaag
     2521 ccatcgcata tttgggcatc cacaccacaa tcacataaaa ggtcgagaat gcggctacac
     2581 cgaacagtac cgatgaaaca ccttccagcc agactaacgg tttgctgttc atttgcggta
     2641 attcattagc tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca
     2701 gcgtgatcag tacaaacaga atacccggaa taatcagccc atagccgtac cagatattat
     2761 tgagcaacat atagctcacc agcattgggt agaacatttg cccaaatgac accatcgctt
     2821 taaccagtat gaccgccgaa ccagaggctt tcggaaagca ttccatgagc gcggggtagc
     2881 cacccgtatc cagcgctgag ttagcgatac ctacgcacac tgccagaccg taggcgagag
     2941 ttaaattcgg gcaagcggga ataccaaaga agaatagcag atacattatt actgccatta
     3001 atatcaccgc ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca
     3061 atcgccccaa accgataccg gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact
     3121 tttctgccag agatgtcata ttttgggcaa gcgtaataac actaatgccg tgcaggaaat
     3181 agctgaagta aatacaaaga acagccagga taaatggcgt gctgaaagcc ttattttgag
     3241 acattttcac agctccttgc tgaaagataa tttcgttacc gtacgtattg ttctccgacg
     3301 gtttacataa ttactatact gaagatgatt ttccctgggt acatggcata aagtttatgt
     3361 ttccaggaat attgcatgtc atagtttgtt tttgatcgcg attttcctta gttagcgcag
     3421 aaagagttat ttatccgtaa gatcttgcat gacctgaaaa gaatattgca ttacccaccc
     3481 ggagcgacat tttcctttag cgagtgatgc cgcgtttttt tgcggctgcg taagccgata
     3541 aacagtgcca gtaaaaaacc gatggcagcg atggcggtat cgaaccacat aatatcggca
     3601 atacttcttt gggacagatg agccgtaatc aacggaatag taaaggtcgc aatactgccc
     3661 gcactgtaat agatccctgt agctttacct ttagcgtaag ggaaacgttc agccattaac
     3721 gtcaggccaa tttgcacaac acctccagca gaggtaaaac caatgacaaa agcaaatatt
     3781 atcaccacat aaaatgtggg atgcaggcag acggtaaaca gagcaataaa tgagataaag
     3841 gtgtacagca tcagtaatgt tgttgggcga acggtattac gaatgagtgg agcggtaata
     3901 aatacacaaa gcagcgaacc cacggtgtag atactgagta gtttgatcga catagtgtat
     3961 gacatgcctg caacaaattg tccgtactgt gccagccact ggctaaccag ataaaacgtt
     4021 gccattgaga tatagccata taaggtataa ctggctaaat cgataattga acagcgatgt
     4081 tccgtagagc tggtggtttt ctttatgaca ggtaagcgac gacccggatg gggtgggaac
     4141 gtacaacggt ataaaaacag agcgttaata aacataatgc ctgcagcaat cataaaggac
     4201 caaccgaacc acagttcagc ccacactaac aggctaatga ttagcggtaa taaaaattgt
     4261 ccgctggaaa caaatgcttt aattaaaata ttggctgtcc caggtgagcg tggaaaagct
     4321 tccatcaaac tgggataagt gcctgcatcg agaaaactgt ttgccattcc cgccagaaag
     4381 ccaaaaacat aagcgataat gatgttattg gtctgcagga tgccaaaaaa gaaggccata
     4441 tagcagcaca tcccgagcat gataaaaggg cggcgaccaa agcgatcgga taataatcct
     4501 gcaaaaagca agacacttaa tcgaccaatg cccagcgatg agataactat cgagacaccc
     4561 gcggcattag tctgccaaag tgtctccagc gaggccatat tcaggcccat caaaatgacg
     4621 cccataccat gcaccaggta attaaaatac aacccaagtg cggtagggta atagggattt
     4681 ttcataggtt ctacttagca aaggtgcttt aaaaagtgta gcgatgttgt gtcagtatcc
     4741 attcattatt gttgtaacaa cgacaattcc tttctgtgga aaaattaaca aagccgctcc
     4801 agatcgtgtg catataattc agcggctacc gattgataga tcttccagtc aataaaattg
     4861 ccatcagggt agacttgttg aaacgcggtt aagtcgggga aaaaacgcat ggtgttgttg
     4921 ccgatgcggt tatttatttt ctctatgatg gcatgaagat gtttttttcg ttgctgacgc
     4981 atagccaaca gttgctcgac tacacaatca ggaatggcgc atttgccatt ttcccactga
     5041 cgccagcttt cactgtcacc ggtctgggca atccaggtcg cgcattcatc aatagtcatg
     5101 gcaaaaatat ggcggagtgc ctggagttca taagcgttca tcgtgtaatg gctgcatgaa
     5161 ataaacaata aaatataaat aacattatca gcctgctgac ggcttgaaga gagtcacagt
     5221 atcttgtgca acattattgg aaagagtgag gggcgaggta gcgaagcgga aaaatgttaa
     5281 aaaaaagccc atcgtgggag atgggcaaag actacacaca gcaattcgtt gtttcactca
     5341 ggggatttcc atgcttataa atcaatatgt tgatttataa ccgtgagcta atagtaggca
     5401 tacggacttt ttgcgtcgat cagattcgtc tcattagttt aaaaactgta aagaatttgc
     5461 gacaggtact acc
//
LOCUS       DAZPLI010000221         5355 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZPLI010000221 DAZPLI010000000
VERSION     DAZPLI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.223"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHUU3_004000"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHUU3_004000"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR2083941.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHUU3_004001"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHUU3_004001"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR2083942.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3390)
                     /locus_tag="ACHUU3_004002"
     CDS             complement(2698..3390)
                     /locus_tag="ACHUU3_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFR2083943.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHUU3_004003"
     CDS             3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHUU3_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR2083944.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1368 a         1385 c         1268 g         1334 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt gcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac
     3061 aaccggctta ccttccagta gatcttccag tttttcactg tgcgccatca accacattac
     3121 caggcggtat aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc
     3181 atcataaaac gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa
     3241 cagcgacatc tgtcgcacac cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac
     3301 aaaggtataa agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa
     3361 tgccatacgg tggagatcga acgccttcat ttttatgctc ttatcgtggt ttatccgcta
     3421 aaagcatagt caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc
     3481 gtattattgc gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc
     3541 ggcaaagatg ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc
     3601 ggctttcaga ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat
     3661 attcgcgaca aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg
     3721 gcgctggctt ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct
     3781 gatttctggc tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa
     3841 tggttcccac tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc
     3901 gcgttttatg atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt
     3961 aacgaagatc gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt
     4021 tatattccga tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat
     4081 acccgcaacg aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac
     4141 cgcattattg ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac
     4201 ccagcagtag ttgaagccat cgaaacgctg gaagcggagg gtttcccaat cttcgcttat
     4261 gacggttcgc ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac
     4321 ggtacctgct ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc
     4381 gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc
     4441 ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc
     4501 ggtttaatct cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat
     4561 ttctccggca ccacggaaga agagtttgct acgctgatgg ctatcttcaa caaagaagat
     4621 aaagaagttt atattgccga ttacgagcat ctgggcgttt atgcttgccg tattatcgtg
     4681 cctggcatgt ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc
     4741 agccatttac gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac
     4801 ctgaacctca tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag
     4861 ctgttgggtc tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta
     4921 aaagccatgc tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg
     4981 acgatggagt ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg
     5041 caaacgttgt tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg
     5101 tttgttcgca tgtacggcgc agatgccgta gaagccgcca gtgcggcaat gagcggcgaa
     5161 gcggcgtttt acggcctgca accggtagat agcgatctgc acgcgtttgc tgcacatcag
     5221 tcgttgttga aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa
     5281 aatcaaatag cctacgcaat gtaggcttaa tgattagtct gagttatatt acggggcgtt
     5341 tttttaatgc cccgc
//
LOCUS       DAZPLI010000222         5197 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZPLI010000222 DAZPLI010000000
VERSION     DAZPLI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.224"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..69
                     /locus_tag="ACHUU3_004004"
     CDS             <1..69
                     /locus_tag="ACHUU3_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083945.1"
                     /translation="VYWVIAVISVVALFLTAKVKDV"
     gene            complement(118..723)
                     /gene="leuD"
                     /locus_tag="ACHUU3_004005"
     CDS             complement(118..723)
                     /gene="leuD"
                     /locus_tag="ACHUU3_004005"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR2083946.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(734..2134)
                     /gene="leuC"
                     /locus_tag="ACHUU3_004006"
     CDS             complement(734..2134)
                     /gene="leuC"
                     /locus_tag="ACHUU3_004006"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR2083947.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(2137..3228)
                     /gene="leuB"
                     /locus_tag="ACHUU3_004007"
     CDS             complement(2137..3228)
                     /gene="leuB"
                     /locus_tag="ACHUU3_004007"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR2083948.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(3228..4799)
                     /gene="leuA"
                     /locus_tag="ACHUU3_004008"
     CDS             complement(3228..4799)
                     /gene="leuA"
                     /locus_tag="ACHUU3_004008"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR2083949.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPLEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(4892..4978)
                     /gene="leuL"
                     /locus_tag="ACHUU3_004009"
     CDS             complement(4892..4978)
                     /gene="leuL"
                     /locus_tag="ACHUU3_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR2083950.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
BASE COUNT         1114 a         1386 c         1382 g         1315 t
ORIGIN      
        1 gtctactggg taattgcggt tatttctgtt gtcgcattat ttttaaccgc aaaggttaaa
       61 gacgtttgat gacgtggacg atagcggaaa gcccggtcat ttgaccgggc aaggggatta
      121 attcataaac gcaggttgtt ttgcttcata agcggcaatg gcgtcgtcgt gctgcaaggt
      181 aagcccaata ctgtccagac cgttcatcat gcagtggcgg cggaaggcat cgatggtaaa
      241 gcgataggtt ttctctcccg ctttcacctc ttgcgcttcc agatccacgt cgaaatggat
      301 ccccggattg gctttcacca gcgcaaacag ttcgtccact tctgcatcgc ttaatttcac
      361 cggcagcagc tggttgttaa agctattgcc gtagaagatg tcagcaaaac tcggcgcaat
      421 caccacttta aagccgtagt cggtcaatgc ccagggcgcg tgctcacgcg aggagccaca
      481 gccgaagttt tctcgtgcca gcaaaatgga agcgccctga tactgcggga agttcagcac
      541 gaagtccggg tttggctgtt ggcctttttc atcgagaaaa cgccagtcgt taaacagatg
      601 cgcgccaaaa cccgtacggg tcactttctg caaaaactgt ttcgggatga ttgcatcggt
      661 atcgacattg gcggcatcca gcggaaccac caggcctgtg tgtttgataa atttctctgc
      721 catggtgtgc tccttattta atgttgcgaa tgtcggcgaa atgtccggtc acagcagcag
      781 cggcagccat tgccgggctg accagatgcg tgcgcccgcc gcgcccctgg cggccttcaa
      841 agttacggtt gctggtggat gcacaacgtt cgcccggatt cagacggtcg ttgttcatcg
      901 ccagacacat tgagcagcca ggcaagcgcc attcaaaacc ggcttcaata aagattttat
      961 ccagaccttc cgcttccgcc tgggctttta ccgggccaga gccgggaacc accagtgcct
     1021 gcacgcctgg cgcgactttt cgccctttgg cgatctccgc tgccgcgcgt aaatcttcaa
     1081 tgcgcgagtt ggtacaggaa ccgataaaca ctttgtcgat agccacttcg gtcagcggaa
     1141 tgcccggttt cagccccata taggccagcg ctttttctgc cgacgcgcgt ttaaccggat
     1201 cggcaaacga agccggatcg ggaatattgt cgttcacgga aatcacctgg cctggattgg
     1261 tgccccaggt gacctgcggt gaaatttctt ctgcttgcag agtgacaacg gtatcgaaag
     1321 ttgcgccttc gtcggtttgc agggttttcc agtaggcaac ggcgtcgtcg aaatctttgc
     1381 ctttcggcgc atgcaaacgg cctttgacat agttaaaggt ggtttcgtcc ggtgcaacca
     1441 gaccggcttt tgcgcccatt tcgattgcca tattgcacag ggtcatacga ccttccatgc
     1501 ttaaatcacg gattgcttcg ccgcaaaact ccaccacatg cccggtgccg cctgcgctac
     1561 cggttttacc gataattgcc agcacgatat cttttgcggt aatgcccggc gcggctttgc
     1621 cctggacttc aattttcatg gtttttgcgc ggccctgttt cagggtttgc gttgccagta
     1681 cgtgttcaac ttcggaagtg ccgataccaa aggccagtgc gccaaacgcg ccgtgggtgg
     1741 cggtatgcga gtcgccgcag acaatggtca tccccggcaa ggtgacgccc tgttccggcc
     1801 ccattacgtg gacgatcccc tgatacgggt gattcaggtc atacagttcg acgccaaatt
     1861 ctttgcagtt tttaatcagc tcctgcatct ggatgcgcgc catttcacca caggcattaa
     1921 tgtctttcgt ctgggtagag acgttgtgat ccatagtggc gaaggttttg cccggctgac
     1981 gtaccgggcg accgtgggcg cgcagaccat cgaacgcctg cggtgaggtc acttcatgca
     2041 ccaggtggcg gtcgatatat aacagcgggg tttcgttttc ggcttcgtac actacgtgag
     2101 cgtcgaataa tttttcgtat aacgttttag ccatgattac accccttctg ccacatagcg
     2161 ggcaatgata tcgcccattt catcggtact aacggcggca gcgccacggg ctaaatcccc
     2221 ggtgcgaatg ccttcttcta atgcgcggtt aatggcgcgt tcaatggcgc aagccgcatc
     2281 atcggcatcc aggctgtagc gcagcagcag tgccagcgac agaatttgcg caatcgggtt
     2341 ggcgatgttt ttgcctgcga tatctggtgc tgagccgcct gccggttcat acagaccaaa
     2401 accttgctcg ttcaggctgg cggaaggcaa catccccatc gagccagtga tcattgcgca
     2461 ctcgtcagac agaatgtcgc caaacaggtt ggagcacagc agaacgtcaa actgtgatgg
     2521 atctttaatc agctgcatgg tggcgttgtc gatgtacata tgcgccagtt cgacatccgg
     2581 gtattccgtg gcgatctcgt tgacgatttc acgccataaa atagaggatt gcagcacgtt
     2641 agctttgtcg attgaggtga ctttgtggcg acgtttacga gcagattcaa acgcgatgcg
     2701 ggcaatacgc tcgatctcaa aacggtgata cacctcggta tcaaacgctt tttcatattg
     2761 tccgctacct tcgcggcctt ttggctgacc gaaatagatg ccgccggtca gttcgcgcac
     2821 gcacaggatg tcgaagccgt tagcggcaat gtctgcacgc agcggacaga atgcttccag
     2881 cccctgatac agttttgccg ggcgcaggtt gctgaataat ttgaagcgct tacgcagagg
     2941 cagcagcgcg ccgcgttctg gttgctggtc tggtggtaaa tgttcccact tcgggccgcc
     3001 taccgagcca aacagcacgg catcggcttg ctcacaacct tcaaccgtcg caggcggcag
     3061 tggttgcccg tggttatcaa tggctgcgcc gcctacatcg taatggctgg tggtgatgcg
     3121 catcgcaaag cggttgcgca cggcattcag cactttcagc gcctgggtca tcacttccgg
     3181 accaataccg tcccccggca atacggcaat atggtaattc ttcgacatca cacggtttcc
     3241 ttgttgtttt cgttgtgttg agctttgcgt tgcaactctt tttcgacttc tgcggcacgc
     3301 cagatattgt tcagtacgtg caccatggct ttggcggagg actcgacaat atcggtggcc
     3361 aggccgacgc cgtggaagcg gcgaccgttg tagttggcga caatatccac ctgacccagc
     3421 gcatctttac cgtgaccttt ggcggtcagg ctgtatttca ccagttcgac gttatagtca
     3481 gtgatgcggt taattgcctg atagacggca tcgaccggac cgttaccgtt ggcggcttct
     3541 gctttgactt cttcgccaca ggccagtttg acggcggcgg tggcgatatc gttagagcca
     3601 gactgcacgc tgaagtaatc cagacggaaa tgctccggct cttcttgctg cttaccgatg
     3661 aaggccagcg cctccagatc gtaatcaaac acctgacctt ttttgtccgc cagcttcagg
     3721 aaagcatcgt acaaattgtc taaattatat tcactttctt tataccccat ctcatccatg
     3781 cgatgtttca ccgccgcacg ccccgaacga gaggtcagat tcagctggat ttggttcaga
     3841 ccaatagatt ctggtgtcat gatttcgtag ttttcgcggt ttttcagcac gccatcctgg
     3901 tggataccgg aggagtgagc gaatgcgctg ctgccaacaa tggctttgtt tgccgggatc
     3961 ggcatattac aaatctggct aactaactgg ctggtgcgcc atatctcctg gtgattaatg
     4021 gcggtgtgga cgttgagaat atccttacga actttaatcg ccatgatgac ttcttccagc
     4081 gaacagttac cggcacgctc gccgatcccg ttcattgcac cttccacctg gcgtgcaccg
     4141 gcatgtaccg ccgccagtga gttgccgacc gccaggccta aatcgtcgtg ggtatgtacg
     4201 gaaataatgg ctttgtcgat gttaggtacg cgttcataca ggccgctgat gattccggcg
     4261 aactcaagcg gcatggtgta gcccacggtg tccggaatgt tgatggtggt ggcaccggca
     4321 ttaatcgccg cttcgaccac tcgcgccaga tcggcaatgg gtgtacgccc ggcatcttcg
     4381 caagaaaatt caacatcatc ggtgtaatta cgggcgcgtt tcaccatata gatagcgcgt
     4441 tcgatcacct cgtccagggt gctgcgcagc ttggtggcga tgtgcattgg ggaggtagca
     4501 ataaaggtat gaatacggaa ggcttcggcg actttcaggg attcggccgc cacgtcgata
     4561 tctttttcca cgcagcgagc taacgcacat acgcggctgt ttttaacctg gcgggcgatg
     4621 gtttgcaccg attcaaaatc gcccggcgaa gagacgggga aaccgacttc catcacgtca
     4681 acacccatac gctcaagggc cagcgcaatt tgcagttttt ctttcacact caagcttgcc
     4741 tgtaacgcct gttcaccgtc gcgcaatgtg gtatcgaaaa taatgacttg ctggctcatg
     4801 gtttaggtcc ttgtctcttt tagagcgcct cgcttcgggc ataaaaaaac ccgcgcaatg
     4861 gcgcgggttt tttgtttgac tgcgtgctgg cttaatgctg gatgccgctc actcgtctac
     4921 cgcgcaaaga agatgcgttt agtagtagta gaccgataaa gcgaacgatg tgagtcatta
     4981 aatcagctcc agatgaatgc gatatgcttt tagagttact ggatacaaaa acggatgtca
     5041 accctgacgc aataaaaacg tcccgccagc gtgagttctg catccgtaaa attagctaat
     5101 tgtgctgcgg tggttaaagt aagcgatatt aatttctgct taactaccga cgcttttcat
     5161 cggttgacat atttcagcat aaatttttgc atctaat
//
LOCUS       DAZPLI010000223         5148 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZPLI010000223 DAZPLI010000000
VERSION     DAZPLI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.225"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(44..1048)
                     /gene="cra"
                     /locus_tag="ACHUU3_004010"
     CDS             complement(44..1048)
                     /gene="cra"
                     /locus_tag="ACHUU3_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR2083951.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1130..1216)
                     /locus_tag="ACHUU3_004011"
     CDS             complement(1130..1216)
                     /locus_tag="ACHUU3_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR2083952.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1228..1719)
                     /gene="ilvN"
                     /locus_tag="ACHUU3_004012"
     CDS             complement(1228..1719)
                     /gene="ilvN"
                     /locus_tag="ACHUU3_004012"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR2083953.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1722..3446)
                     /gene="ilvI"
                     /locus_tag="ACHUU3_004013"
     CDS             complement(1722..3446)
                     /gene="ilvI"
                     /locus_tag="ACHUU3_004013"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR2083954.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNVTVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3565..3609
                     /locus_tag="ACHUU3_004014"
     CDS             3565..3609
                     /locus_tag="ACHUU3_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083955.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3764..4708)
                     /gene="leuO"
                     /locus_tag="ACHUU3_004015"
     CDS             complement(3764..4708)
                     /gene="leuO"
                     /locus_tag="ACHUU3_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR2083956.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSSLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         1397 a         1338 c         1139 g         1274 t
ORIGIN      
        1 gcgccagagg gaaattcacc tggcgcgtat ttttgttcgc ggcttagcta cggctgagca
       61 caccgcggcg atagagatta cgtttaatgc gcgttaaacc aggttttggc ttacgcggtt
      121 cgtccaggct tgccaggaca atctccagca cacgctctgc gacatcgcgg tgacgttgag
      181 ccactgccag caccgggcac tgtaagaagt cgagcagttc gttatcgcca aaggtggcaa
      241 ttgccaggtc agaaggcagt ttaccatcgc gacgtagcgt gacatccatc accccttgca
      301 acaacgcaaa cgacgttgtg aacagcgcct gcggcatcgg atgcgtttcc agccattttt
      361 cgaataactg ggcagccacc tcccgctcat agctgttggc atacaggaaa tgcacttcgc
      421 gcggatcatc tttccaggca gtacggaaac cttgttcacg caggaagctg acagaaagct
      481 ccggcagcgc gccaagataa agcaccgtct cggcgggaaa cttacgtaac tcttccgcca
      541 gcatttcggc atcatcctga tcggcaccaa ccacgctggt gaagtgttca cgatcgaggg
      601 cgcggtccag cgcgacaatc gggaacgggt cgttagccca gcgttgataa aaaggatgct
      661 caggaggcaa cgacgtcgaa acaataatgg catcgacctg acgctgtaaa aggtgctcaa
      721 tacagcgcat ttcgttgtct ggctgatctt ctgagcaggc aattagcagt tgataacccc
      781 gttgccgcgc ctggcgttca agatagttag cgatgcgggt atagctggtg ttctccagat
      841 cggggatcac aagaccaata gaacgtgtgc gtccagcacg aagcccagct gccacggcgt
      901 tcgggtggta attgtgctca cgcaccacag ccatgacttt ttcaacggtt ttgtcgctca
      961 cacggtattg cttcgctttg ccgttaataa catagcttgc agtggtccgc gacactcccg
     1021 ccagccgagc gatttcatcc agtttcacaa ttgccccttg cgtaaaatgt aaaaaccata
     1081 gccattgtac tggtatgggt taaattcatt aacatctaag cgcataaaat taactgcggc
     1141 aactgctttt attccaggtt cccactgatt tcgcaaaaaa agcccaacgt gacatgttgg
     1201 gctgcaaatt gcgcattcag atcattctca acgcattatt ttatcgccgc gcgaaagtcc
     1261 gaccacacca gaacgagcaa cttccacaat tttcgccacc tcgcgaatcg atgctaaaaa
     1321 tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta taaagcgagg gtgtgacatc
     1381 gataatttgc ccacggaata tttccgtatt acgtttcact tcgtcacgcc cgtaaccgct
     1441 ggcctgaatt ttcaccagca tgatttcccg ctcaacatgc gcgccctgcc ccaactcact
     1501 cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg atctgctcaa gtactttttc
     1561 atcgcccacg gtctggatgg tcatacgcga taatgtcgga tcatcggttg acgcaacggt
     1621 caggctttca atgttgtagc cacgctggga aaaaaggcca atcacgcggg ataacgcgcc
     1681 tgattcattt tcgagtaaga ctgataatat ccggcgcata atcaggttct ctccgttttg
     1741 cttaaccaca tttcatccat tccgcccccg cgaatctgca tcgggtagac gtgctcgctg
     1801 ccatcgacgg taacatcaac aaacaccagg cgattattgt gcacctgttc cagcgcctcg
     1861 ctaagtttgc tttccagctc atgcggatga gaaatctgga tcccgacgtg accataggct
     1921 tccgccagac ggacgaaatc gggtagcgat tgcatataag attgtgaatg acggccggaa
     1981 tagatcatgt cctgccactg cttcaccatc cccagatagc ggttattgag attcaccacc
     2041 agtacgggca actcgtattg caacgcggta gacagttcct ggatgttcat ctgaatactg
     2101 ccgtcgccag tgacgcaaac cacggtttct tctggcaacg ccattttgac gcccagtgcc
     2161 gcaggtaaac caaaacccat cgtgccgagg ccaccggaat tgatccagcg acgcggtttg
     2221 tcgaatggat aataaagtgc agcaaacatc tggtgctgcc cgacatcgga cgtcacgtaa
     2281 gcgtctccct tcgtcaaccg ccaaagagtc tcgatcaccg cctgcggttt aatcttttca
     2341 ctgtgagtgt catatttcag gcactgacga gcgcgccact gttcaatttg ctgccaccag
     2401 tcgcggatct catccagtgg ttgatgggcg gattcttgcg acaagagttc aagcatttgt
     2461 tcgaggacct ggcgagcatc ccccacaatc gggatatccg cagtcacggt tttagaaatg
     2521 gaagtaggat caatatcgat atgcagaaca gtgacatttg ggcagtactt tgccagattg
     2581 ttcgtcgttc ggtcatcaaa tcgtaccccg acggcgaaaa tcacatccgc gttatgcatc
     2641 gtcatattgg cttcgtaggt accgtgcatt cccagcatgc ccagtgcctg acgatgcgtt
     2701 gccggaaacg ctcccagccc catcaatgag gaaacaacgg gcagattcaa cgcctccacc
     2761 gtttctttca actgctgatg gcagcccgcc gtgattgccc caccgcctac gtagacaacc
     2821 ggtttttttg ccgctaccag cgtttgcaga gcgcgcttaa tttgcccttt atgtccggta
     2881 gtagtgggat tgtaagaacg catactgacc gactccggcc agacataggg taatttgttc
     2941 gccggattaa gaatatcttt cggtaaatca acgactactg gtccaggacg accacttgcc
     3001 gccagccaga aagccttttt cagcacctgc ggaatgtctt ccgtttgctt aaccagaaaa
     3061 ctgtgtttaa ccactggtcg cgaaatcccc accatgtcgc actcctgaaa ggcatcgtaa
     3121 cctatcaacg aggtcgctac ctgcccggaa aggacaacta atggaatgga atccatataa
     3181 gcggtggcga tgccagtaat cgcattggtc gcccctggac ccgacgttac cagcacgacg
     3241 ccgacttccc cggtcgcgcg cgccagacca tcggccatat gcactgccgc ctgctcatga
     3301 cgaactaaaa catgatcgat accacccacg gtatgcaatg catcgtaaat atcaaggact
     3361 gcgcctccgg gataaccgaa tacttgttta acgccctgat cgataagcga tcggacgacc
     3421 atctcggctc cagacaacat ctccatggcc tgcctcactg tttgacggaa aaaatgtgta
     3481 aaacacatta accgtccgat cttgcttaag caattggcag ccagagacat aaacgcaggg
     3541 taataagaat aaacaggagg aaaggtgaaa agaaacgata aaatcctcca ttacgaagga
     3601 ttaaattgat aataaatcgg ctgaatccca caacttacag caatttctct atttttttaa
     3661 ttaatcctaa aatagagagc ccacacattc agaataaaat tctacattca ataaaaaata
     3721 cagaataaac cagacattca tgtctgacct attctgcatt cagttagcgt ttgcaaattg
     3781 agactaattg ctcttccatc cactgatggc ctttatcgcg cccggcagct tcatgccagg
     3841 agagataaca ggttctgcta ttttgtttta acggcagcgg taatacctgt aattctaagg
     3901 attcagcgaa ctcttcagcc agccaacgcg gcgcaatagc gaccaaatgc gtttgcgaca
     3961 ccacgctaag tacgctcatc attgccatgc cctgatacgc gatactggct tgcttatcta
     4021 ccgtgtcata ccaaggttga ctaaatgacg cgaaacgatc gagcgaaacc gccgcatgtt
     4081 gttcgttata aacatcatgt ttcagtaacg ggcccttaat tgttggatga tttttgctgg
     4141 ctaccagcac catttcatct ttaaataatg gcacgctggt aaattcagga cgatggaagt
     4201 cttcataact aatcacaaac tccgtttcct gataacgcag ctgatgttca gtgttctgat
     4261 ttaatgaaga cttgaacata acatgtatat ttggcgcaat ctgctcaatg tgattataaa
     4321 tctgcgaggt cagaatgctg tctaacgagc tgcaaacaca aagatgaaat acacgttcac
     4381 tgctcgcggg ttcaaaacct gaaccaggca attcattttg tactagttgc aatgcctgac
     4441 gaactgagcc aaaaagttga aatgcgcgag cagtcggttg aataccacgg ccataacgaa
     4501 caaaaagctc gtcattaaac atcaccttca ggcgtgcaac agcgttactg accgcaggtt
     4561 gcgacatacc cagaacatga gcggcacggg taatgttttg ctcctgcatc acggcatcga
     4621 aaacggttaa taagttgaga tcgaccatgc gtagctgtgg cttgcttaac tccgccgtct
     4681 ctgaatgatc tgtttgtacc tctggcatac ttaactccac tgtcacactt aactcccttt
     4741 cccttattgg aatgcagaga aataatcctg aagaatatga tttatcatgc atataaaata
     4801 agaaaaagca aaatgagtaa aattcggaaa acataaagat acgggttctg ataaaaacag
     4861 aatccattgc cgtggaatga gtcatttacc acagcataag aatccataat gtttttgttg
     4921 cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca tctacaaaat ggattaaatg
     4981 tgcttattta ataattaatt tatatattta atgcattaat tcttaacatt aattgatcaa
     5041 taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca tataattcaa tacaaatcat
     5101 caggataggt tttgcaacgc gtgcattttg tccccttttt cctcgttg
//
LOCUS       DAZPLI010000224         5054 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZPLI010000224 DAZPLI010000000
VERSION     DAZPLI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.226"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            194..1168
                     /gene="hemB"
                     /locus_tag="ACHUU3_004016"
     CDS             194..1168
                     /gene="hemB"
                     /locus_tag="ACHUU3_004016"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR2083957.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1274..2125)
                     /gene="tauD"
                     /locus_tag="ACHUU3_004017"
     CDS             complement(1274..2125)
                     /gene="tauD"
                     /locus_tag="ACHUU3_004017"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR2083958.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2122..2959)
                     /gene="tauC"
                     /locus_tag="ACHUU3_004018"
                     /pseudo
     CDS             complement(2122..2959)
                     /gene="tauC"
                     /locus_tag="ACHUU3_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
     gene            complement(2956..3723)
                     /gene="tauB"
                     /locus_tag="ACHUU3_004019"
     CDS             complement(2956..3723)
                     /gene="tauB"
                     /locus_tag="ACHUU3_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR2083959.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3736..4698)
                     /gene="tauA"
                     /locus_tag="ACHUU3_004020"
     CDS             complement(3736..4698)
                     /gene="tauA"
                     /locus_tag="ACHUU3_004020"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR2083960.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1200 a         1436 c         1254 g         1164 t
ORIGIN      
        1 ataataacca agagctataa ataaagatta aaaatatttt cagtgaatat gaaattattg
       61 ctatttctat tacaaagatg tcctggtctc tctttcattc tcttcttgtc aacatcgcga
      121 caactttcgt aaaacactcc tgccccgctt caggtatact gtgcacctta attccacaaa
      181 catcaggcag accatgacag acttaatcca acgccctcgt cgcctacgca aatctcctgc
      241 gctgcgcgct atgtttgaag agacaacact tagccttaac gacctggtgt tgccgatctt
      301 tgttgaagaa gaaattgacg actacaaagc cgttgaagcc atgccaggcg tgatgcgcat
      361 tccagagaaa catctggcac gcgaaattga acgcatcgcc aacgccggta tccgttccgt
      421 aatgactttt ggcatctctc accataccga tgaaaccggc agcgacgcct ggcgggaaga
      481 tggactggtg gcgcgtatgt cgcgcatctg caagcagact gtgccagaaa tgatcgtcat
      541 gtcagacact tgtttctgtg aatacacttc tcacggtcac tgcggtgtgc tgtgcgagca
      601 tggcgtcgac aacgacgcga ctctggaaaa tttaggcaag caagccgtgg ttgcagctgc
      661 tgcaggcgca gacttcatcg ccccttccgc cgcgatggac ggccaggtac aggcgattcg
      721 tcaggcgctg gacgctgcag gctttaaaga tacggcgatt atgtcgtatt cgaccaagtt
      781 cgcctcttcc ttttatggcc cgttccgtga agctgccgga agcgcattaa aaggcgaccg
      841 caaaagctat cagatgaacc caatgaaccg tcgcgaggcg attcgtgaat cactgctgga
      901 tgaagcccag ggcgcagact gcctgatggt taaacctgct ggagcgtacc tcgacatcgt
      961 gcgtgagctg cgtgaacgta ctgaattgcc gattggcgcg tatcaggtga gcggtgagta
     1021 cgcgatgatt aagttcgccg cgctggcggg tgctatagat gaagagaaag tcgtgctcga
     1081 aagcttaggt tcaattaagc gtgcgggtgc ggatctgatt ttcagctact ttgcgctgga
     1141 tttggctgag aagaagattc tgcgttaatt tttttcctct cagaaagggg gagttaacct
     1201 gttgaaagta cggtgaacgc cccctcaccc taaccctctc cccaaggggg cgagggaacc
     1261 gtccgcactc gtatcacccc gcccgataaa acggtttatc cccaaggatc gtcgcccgat
     1321 gcataatccg ccgctgtggc aggtaatcgg cattggcata gtgctgagtc acgcggttat
     1381 cccaaatcgc aatatcattc ggttgccagc gccaacgcac ctgaaactcc ggtttagtga
     1441 tatgggcgaa taaaaagcct aacaaggctt cgctctcttt ctcgctgaca tcgacaattc
     1501 gcgtagtaaa gccttcattc acaaacagcg cctgtttacc gctcaccgga tgcgttcgca
     1561 ccaccggatg tagcaacgga ggatttttcg cgaccgcctc gcgccagcgt tgatgttcct
     1621 cctcggtttt gcggtatttg tattccggga acgatttacg gaaatcatgc tccgcacgca
     1681 gcccgctcag cagctggcgg aagggaacag agagcgcctc ataggccgca ataccgctgg
     1741 tccagagcgt atcaccgccg gtcgaaggta actctttagc tgccagaatc gcccctgcgg
     1801 gtggcgtttc aataaatgtc acatcggtat gccagttgtc gttatccggc ggattatcgt
     1861 tatgggtatc cagcacgatg atctcgtcaa ccccttcggc atgagggtaa acagggtgaa
     1921 tatgcagttc gccaaaacgc tgggccagcg cgcgttgctg ctgcggcgta atagcttgat
     1981 cgcgcagaaa taccacctga tagcgcagca ccgcatggta aagctgttca aactgattat
     2041 cgcttaacgg gcgcgtcagg tcggcacccg aaatttgtgc gccgatatac ggccccagcg
     2101 gggtaatact cagacgttca ctcattgtac ttctccatgc cagggcgtca gacggcgctg
     2161 taacgcgcgc agacccagtt ctaaaagaaa ggcgataatc gcaatcaccg cgatccccgc
     2221 cagtaccacg tcagtcgcga gaaattcacc cgctgactga accataaatc ctaaaccgcg
     2281 tgtcgcggca atcagctccg ccgctaccag cgtagaccag cccacaccca gaccaatgcg
     2341 taatccggtg aggatttccg gcagcgcgcc gggcaaaatg acaaaccaca gcacctgcgc
     2401 acggctggca cccagcgact gggcggcacg aatgcgaacc tgctgcgcgc ttttcacccc
     2461 cgccagcacc gacatcgcca ccggtgcaaa aatcgctaaa tagatcaata agatcttcga
     2521 ggtttcacca ataccaaacc agatcaccat cagcggcaaa taagccagcg gcggcaccgg
     2581 acgataaagt tcgattaccg gatccagaat gccgcgcacc gtcgggctta gccccatggc
     2641 gatcccgacc ggaataccga tcaccaccgc cgcgagtagc gccagcacaa tgcgcgtcag
     2701 gctggctgcc agatgctgcc agatgctgcc acagcgtggc gtccataaat ccctgcggtc
     2761 cggcaatggt gagcagtttt gccagtactt gttgcggcgg cggcaaaaac agcgggctaa
     2821 tcagttgcag cgccgccacc gtccaccata cagtgagtaa aaccgctaac gtgccaatgc
     2881 ttaaggtcac ctgacgcgag agcggccagc gccatttcag ccgccgcgaa tgcagttttt
     2941 cattaatgag cacactcatg agaacgcctc ccgttgctca aatacgcggc ttaaaacata
     3001 ttcgcgcatg gcgatgaatt gtggatcgga cttgatgctg cggctcgact ctcccgcaac
     3061 aaagcggcga gcaaagttga gcggcagccg ctccagcaca cggccagggc cggatgaaag
     3121 cagaaccagt tcagtcgcca taaacaccgc ttcttctata tcgtgggtaa tcaacagcac
     3181 ctgcttgccc gtctcctgcc agagtttcag cagcagagtt tgcatctggt cgcgggtgaa
     3241 ggcgtccagc gcaccaaacg gttcgtcgag taataacagc tggggattcg ccgccagcgc
     3301 gcgggcaatc cccacccgct gacgttgacc accggaaagc tgccagatat agcgtttttc
     3361 tgcgccttcc agccccactt ttttcagcat ctggtgcgcg atttccagtc gctgcatttt
     3421 ctctatacct gccaattgca ggccgaacgc cacgttgtcc tgtacattgc gccacggtag
     3481 tagcccttca ttctgaaaaa ccaccccgcg ctctgctccc ggtccctcaa tacgcttacc
     3541 cgccagttga atgctgccat gctgataagg cacaaaaccg gcaatcagat tcagcagggt
     3601 ggttttaccg cagccggacg gccccagcac caccagtagc tcgccgcttt ccagcgtcag
     3661 gttgatatct tccagtgccg gtttgccgcc ataatcggca tagagatgag agatttgcag
     3721 catctgcgcc tccttttatt gcacgaagcg cgaggtaacg tactggctgt aatcattcgc
     3781 tacagccggg accttgccct gctctttcaa aaactgcgcg gtgtcgatga ttgctttgtt
     3841 caccggtccg gtcagttctg ccgtttgttg ctgcggcgtc agataggtat tccccttcac
     3901 cagccccgga acgtcacctt ccggcacgcc gcttaaacgc gccagtttgc tgatgttttc
     3961 cggctgtttc agccacgcgt ctgggttagc aatgtacggt tgctgagcat cgatggcgct
     4021 tttagcgaac gctttcacga cctcaggatg tttctcggca aaatctttgc gcaccaccca
     4081 gacgtccagc gttggcgcac cccactgccc gacctgttca gaatcggtca acaccttgcc
     4141 gtctttttcc agggcgttaa ccgccggtgc ccagacataa gcaccatcaa tatcaccccg
     4201 ctgccatgcg gcgataatcg cgggcggctg caggttcaca atctccactt gccctggttt
     4261 aatcccccag tgtttcagcg ccgccagcag gctgtagtgg gtggtggaga taaacggtac
     4321 ggcaatgcgt ttgccaatca aatcttcggg tttgctgata gttttcttta ccaccagcgc
     4381 ttcggagtta cccagttttg acgccagcaa gaagacttca atcggcacct gttggctggc
     4441 tgcaaccgct aatgggctgg aaccgaggtt gccgatttgc acgtcgccag aagccagcgc
     4501 ccgcacgatg ctggctccgc tgtcaaactt acgccagtcc acgctcgccc cgctctcttt
     4561 agcaaaggtg ttgtcggcct gagccacttt cgccggttcg gctgaggttt gatacgccac
     4621 ggtgacgttc accgcctgtg cctgaaaagc gatgaatgcc agtgcggcaa gaagtgtgtt
     4681 acgcgatgaa attgccatga ttgtctgctc ccctgtcttg ttatgggagc agtattcagg
     4741 aataaaaaca ttcattaaaa gaattagtcg ttatcgcaca gatgatttta ttcttagcaa
     4801 aaaaacggca aaggaaagat taaatatgca attactccgt tctaacaatt gggaaaatta
     4861 ctacacttca ttttagtgat gcataaacaa ccacattacg agatttatta cttcattgtt
     4921 tctatcaata catataataa aactattata ttcgcattgt atatatcact gtatcattct
     4981 caatcactcg catattacat tcacataatg tatataaaca atacttgttc acagaacata
     5041 ttttttaaat gcaa
//
LOCUS       DAZPLI010000225         5015 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZPLI010000225 DAZPLI010000000
VERSION     DAZPLI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.227"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            12..754
                     /locus_tag="ACHUU3_004021"
                     /pseudo
     CDS             12..754
                     /locus_tag="ACHUU3_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(796..1248)
                     /locus_tag="ACHUU3_004022"
                     /pseudo
     CDS             complement(796..1248)
                     /locus_tag="ACHUU3_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1312..1458
                     /locus_tag="ACHUU3_004023"
                     /pseudo
     CDS             1312..1458
                     /locus_tag="ACHUU3_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHUU3_004024"
     CDS             complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHUU3_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR2083961.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2777..2851)
                     /locus_tag="ACHUU3_004025"
     tRNA            complement(2777..2851)
                     /locus_tag="ACHUU3_004025"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2816..2818),aa:Arg,seq:cct)
     gene            complement(2927..3859)
                     /locus_tag="ACHUU3_004026"
     CDS             complement(2927..3859)
                     /locus_tag="ACHUU3_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR2083962.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4153..4908
                     /gene="mlaA"
                     /locus_tag="ACHUU3_004027"
     CDS             4153..4908
                     /gene="mlaA"
                     /locus_tag="ACHUU3_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR2083963.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT         1219 a         1165 c         1173 g         1458 t
ORIGIN      
        1 gggtggatga tatggcaact acatgttcag ttatattgat tttggagtcc tttgatgttt
       61 atttcggaaa agagagtgtg tttctggaga gaggttcatc tgtacttgtc gactctagct
      121 ctagagattt tttcctgaca tatcctgaaa gagtgatagt ggcggatttt ggcgctgagt
      181 ttattagtcg ctatttgaaa gctaataact taagggatat ttctgattgt agggaatatc
      241 catcttattt aaaaataaac tttgctgact tcagtttaat taaaggatta attagttggg
      301 ctaatcactg tgctgaatac atagaaattt ttgatgagtc tattgctttt acatgtctct
      361 ctgcattttc ttctgaaaaa caatttggag tatttctgtt tggatgtttg aaaagcacag
      421 ggctaaagtt aaaacgatta ttcatacgga tttatctgca ccatggcgtc ttaaggatat
      481 atcatcaaga ttatatctca gcgaaagttt actaaagatg aaattgaaag aagagggggt
      541 atcattcagt aagatcatac ttgatgagag gatgcaaatg gctgaatatt tactcagcac
      601 tcgttgttat cctattagta aagtagctaa ggtctgtggt tatgccagtg tctcatactt
      661 tacttatgta tttagacgtt attttggtgt ttctccaagt caatactctc agaggagttc
      721 agaaagtaaa attcttactc accagggaat ctgatcattg ttcttgcccc cttatttcca
      781 gacagggggt gtatcttaag ttaacgttac ccgctgacgt cgatattctc gcggagagcg
      841 ataacccagc gcactgtgcg gatggttttc attgtaatgt tcgatcgcca ctgcaagatt
      901 atgcaatgcc gttcttacat tcggtttcgg catgaacgcg atgtagtctt ccttcatcgt
      961 tttcacgaac ctttctgcca ttccattact ctgaggactg ctgattgccg ttgtgcaggg
     1021 ctccagattc aactctctgg cgaactgccg tgtttcatgc gctctatacg ctgaaccgtt
     1081 atctgtcagc cactggatgg actgttccgg tactttatcg ccaaagcgtt tttctatcgc
     1141 tcctaacatg acatcctgca ctgtcgcttt atcgtaacct cccgtgcttg cggcccagtc
     1201 tatggcttca cgatcgcaac agtccagggc gaacgtaaca cgcagttttg aatcgccacg
     1261 ggtttaacag acacctcaga gtcatttaag atggcttaaa gagaggtgcc catgagcggt
     1321 aagcgttatc ccgaagagtt taaaactgaa gcagtcaaac aggttgttga tcgcggttat
     1381 tctgttgcca gcgttgcaac acgtctcgat atcactaccc acagccttta tgcctggata
     1441 aagaagtacg gtccggatta ctccactttt tcatcaagcc aatccgccca ccactgcatc
     1501 atttctctgc gcttatcgag atactgagca tggttgtaaa taccgcgcac agatccgccg
     1561 ttggcatgtg ccagctgcac ttcaatagcg tcagcaggcc attcgtgctc gttcataatc
     1621 gtgctgaatt catgcctgaa tccgtgaccg ctttccagac cttcatagcc gatttgtttg
     1681 atcacaagca ataccgcgtt ctcgcagatt ggcttcttct tatcgttgcg cccggcaaaa
     1741 acaaactctg agactggttt ggtgattgag cttagcgtag tgagaagttc aaccacctgg
     1801 tctgacatcg ggaccacatg aattttgcgt cccttcatca cactggcgtc gatggtgata
     1861 atcctgtttt caaaatcgac gttcttccat agcatggaac gaagctcttt tgttcttagg
     1921 gctgtgtagc gtaaaacttt ggtcgcaatg agcgatacga tacttcctga aaatgttgcc
     1981 agtgctttgt tgaatgcagg catctggtct gctggaagga acgggaagtt cttcttgcgg
     2041 tatcctttca tggcgtctgc aaggtcaggt gccgggttat atttagccct tccggtgaca
     2101 atagcgtaac gaaaaacctc gccgcatctt ctgcgggctt tgttggctcg ctccattgca
     2161 ccgcgatctt caaatctgcg gattacttcc agcagttgca tcggctcaat atcctgaatc
     2221 tcaagaccgc cgatgatggg taaaatgtcg tcatcaaaca ttttggcaag ttcagttgca
     2281 tagcctactg accagacttg cttcttgtgc tcgtaccatt ccttgtaaat cgcactaaat
     2341 gaattgttgt tagacgaagc ctttttcgcc tttaccggaa cgatgccaac cgagatgtct
     2401 ttcctcgcag tccatgcctt atcccttgct tcctgcaaag tcataagcgg atattttccg
     2461 acggtcagga ttttctcctt accgtcaatc ttgtagcgaa gctgccatac ctttttcccg
     2521 gatacaggaa cataaaggta caggccatta ccatcgagaa ggcggtatgg tttttctttc
     2581 ggctttgctg cttcaatctg cttaacggtg agcatgggta aaaatccggt gggtaaaatt
     2641 attttatcca ctttttaccc gtcatggagt gcggctgtca acgatctgac gcgaaccatg
     2701 acgaaccgtg aatatacgga aggcttgata ttcaggggat tttgcggact ggtacggatg
     2761 ggagcgaact gataaatggt gtcccctgca ggaatcgaac ctgcaattag cccttaggag
     2821 gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc cgctaatgga
     2881 gtgcggggta agtacgctgc cgcttgattg cttaaaccct cgccatttat gccgggtttt
     2941 tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct
     3001 tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc
     3061 cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca
     3121 gcgtaccgtt aaacaccaga tagaggattt caacagaacc gaccacgata tgggtggtgt
     3181 cacctagggc aataagccag gtcatcaata taatcaccac aatctttgcc gcacctgctg
     3241 caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca
     3301 tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt
     3361 cttcattgaa gataggcata tattcaaatg cccacgcagc aataccagtc ccgagaatat
     3421 tacccagcag cacgactccc cataaccgca taagtaagcc aacgttgctc attgtcggtt
     3481 tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca
     3541 ttatgacgat aataaaacca aaggtataac cgagattctc cagtaagaag ctgcccggca
     3601 caccttccag ttcgacatga aatatccctt ttgccagtaa cgaagcgccc atcgacagac
     3661 ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct ttttcaccat
     3721 cctggcggat atgctcatga attgccatcg cccgggaggg gagtcggtct tcatctattt
     3781 ctattttttt gccgcgctct ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat
     3841 caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc
     3901 ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc
     3961 ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca
     4021 aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta
     4081 aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac
     4141 agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg
     4201 gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac
     4261 cgcaccatgt acaacttcaa cttcaacgta ttagacccgt atattgttcg accggtcgct
     4321 gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaatg gtttgagcaa ctttactggc
     4381 aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg
     4441 atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg ctttattgat
     4501 gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg
     4561 cttggtcatt atggcgtggg ttatgggcct tacgttcagt taccgttcta cggtagcttc
     4621 acgctgcgtg atgacggtgg tgatatggcg gatggtcttt acccggttct ttcctggctg
     4681 acctggccga tgtctgtggg taaatggacg cttgaaggga tcgaaacccg cgctcagctg
     4741 ctggattccg atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg
     4801 tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg
     4861 aacgcacaag cgattcagga tgatttaaaa gatattgatt ctgaataaga aacaaataaa
     4921 aaaggtgagt cgcaaaactc accttttttg ttgttatccc tcaattacca caagtaaaag
     4981 gagataaacc gacaatgacc cggacgtaat gatgt
//
LOCUS       DAZPLI010000226         4986 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZPLI010000226 DAZPLI010000000
VERSION     DAZPLI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.228"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            149..1252
                     /gene="proB"
                     /locus_tag="ACHUU3_004028"
     CDS             149..1252
                     /gene="proB"
                     /locus_tag="ACHUU3_004028"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR2083964.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1264..2517
                     /gene="proA"
                     /locus_tag="ACHUU3_004029"
     CDS             1264..2517
                     /gene="proA"
                     /locus_tag="ACHUU3_004029"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR2083965.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2632..2707
                     /locus_tag="ACHUU3_004030"
     tRNA            2632..2707
                     /locus_tag="ACHUU3_004030"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2665..2667,aa:Thr,seq:cgt)
     gene            3313..3471
                     /locus_tag="ACHUU3_004031"
     CDS             3313..3471
                     /locus_tag="ACHUU3_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083966.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         1385 a         1085 c         1240 g         1276 t
ORIGIN      
        1 taataaaagc gtaaacaact gccgctaggc ttgctgatcc cgcgcaacaa aacgccatgc
       61 tttgctcgca gatggttggc aaccgacgac agtcctgcta aaacgttcgt ttgatatcat
      121 ttttcctaaa attgaatggc agagaatcat gagtgacagc cagacgctgg tggtaaaact
      181 cggcaccagt gtgctaacag gcggatcgcg ccgtctgaac cgtgcccata ttgttgaact
      241 tgttcgccag tgcgcgcagt tacatgccgc cgggcatcgg attgttattg tgacgtcggg
      301 cgcgatcgcc gccggacgtg agcacctggg ttacccggaa ctgccagcga ctatcgcctc
      361 gaaacaactg ctggcagcgg tagggcagag tcgactgatt caactgtggg aacagctgtt
      421 ttcgatttat ggcattcacg tcgggcaaat gctgctgacc cgtgctgata tggaagaccg
      481 tgaacgcttc ctgaacgccc gcgacaccct gcgtgcgttg ctcgataaca atatcgttcc
      541 ggtaatcaat gagaacgatg ctgtcgctac ggcagagatt aaagtcggcg ataacgataa
      601 cctttctgcg ctggcggcga ttctggcggg tgccgataaa ctgttgctgc tgaccgatca
      661 aaaaggtttg tataccgctg acccgcgcag caatccgcag gcagaactga ttaaagatgt
      721 ttacggcatt gatgacgcac tgcgcgcgat tgccggtgac agcgtttcag gcctcggaac
      781 tggcggcatg agtaccaaat tgcaggccgc tgacgtggct tgccgtgcgg gtatcgacac
      841 cattattgcc gcgggcagca agccgggcgt tattggtgat gtgatggaag gcatttccgt
      901 cggtacgctg ttccatgccc aggcgactcc gcttgaaaac cgtaaacgct ggattttcgg
      961 tgcgccgccg gcgggtgaaa tcacggtaga tgaaggggca actgccgcca ttctggaacg
     1021 cggcagctcc ctgttgccga aaggcattaa aagcgtgact ggcaatttct cgcgtggtga
     1081 agtcatccgc atttgcaacc tcgaaggccg cgatatcgcc cacggcgtca gtcgttacaa
     1141 cagcgatgca ttacgccgta ttgccggaca ccactcgcaa gaaattgatg caatactggg
     1201 atatgaatac ggcccggttg ccgttcaccg tgatgacatg attacccgtt aaggagctgg
     1261 ccgatgctgg aacaaatggg cattgctgcg aagcaagcct cgtataaatt agcgcaactc
     1321 tccagccgcg aaaaaaatcg cgtgctggaa aaaatcgccg atgaactgga agcacaaagc
     1381 gaaatcatcc tcaacgctaa cgcccaggat gttgctgacg cgcgagccaa tggccttagc
     1441 gaagcgatgc ttgaccgtct ggcactgacg cccgcacggc tgaaaggcat tgccgacgat
     1501 gtacgtcagg tgtgcaacct cgccgatccg gtggggcagg taatcgatgg cggcgtactg
     1561 gacagcggcc tgcgtcttga gcgtcgtcgc gtaccgctgg gggttattgg cgtgatttat
     1621 gaagcgcgcc cgaacgtgac ggttgatgtc gcttcgctgt gcctgaaaac cggtaatgcg
     1681 gtgatcctgc gcggtggcaa agaaacgtgt cgcactaacg ctgcaacggt ggcggtgatt
     1741 caggacgccc tgaaatcctg cggcttaccg gcgggtgccg tgcaggcgat tgataatcct
     1801 gaccgtgcgc tggtcagtga aatgctgcgt atggataaat acatcgacat gctgatcccg
     1861 cgtggtggcg ctggtttgca taaactgtgc cgtgaacagt cgacaatccc ggtgatcaca
     1921 ggtggtatag gtgtatgcca tatttacgtt gatgaaagtg tagagatcgc tgaagcatta
     1981 aaagtgatcg tcaacgcgaa aactcagcgt ccgagcacat gtaatacggt tgaaacgttg
     2041 ctggtgaata aaaacatcgc cgatagcttc ctgcccgcat taagcaaaca aatggcggaa
     2101 agcggcgtga cattacacgc agatgcagct gcactggcgc agttgcaggc aggccctgcg
     2161 aaggtggttg ctgttaaagc cgaagagtat gacgatgagt ttctgtcatt agatttgaac
     2221 gtcaaaatcg tcagcgatct tgacgatgcc atcgcccata ttcgtgaaca cggcacacaa
     2281 cactccgatg cgatcctgac ccgcgatatg cgcaacgccc agcgttttgt taacgaagtg
     2341 gattcgtccg ctgtttacgt taacgcctct acgcgtttta ccgacggcgg ccagtttggt
     2401 ctgggtgcgg aagtggcggt aagcacacaa aaactccacg cgcgtggccc aatggggctg
     2461 gaagcactga ccacttacaa gtggatcggc attggtgatt acaccattcg tgcgtaaata
     2521 aaaccgggtg atgcaaaagt agccatttga ttcacaaggc cattgacgca tcgcccggtt
     2581 agttttaacc ttgtccaccg tgattcacgt tcgtgaacat gtcctttcag ggccgatata
     2641 gctcagttgg tagagcagcg cattcgtaat gcgaaggtcg taggttcgac tcctattatc
     2701 ggcaccatta agaaaattaa taacttagct taacttctct aagaaattgt atcttctgtt
     2761 atcacttaca aacgcctata cgttgttatg ggctttggta gtgtaacacg tatgggcgag
     2821 tagttcccag gctcaacctc gtaacaagtt cctttgaagc tggaagacgt gccaccgtct
     2881 ctaaaccttc taccaaaact cttagcaaga tgttccacac ctggcgcggt gtccaatgat
     2941 gctacacgtc gtgagacgtt gcagaggata aacaggctaa atgatgggaa gagttttaag
     3001 tgcaatagtg gattaaagcc cacctcttca aaacctaact tgatcgggaa agaatcaagc
     3061 tctagcacgg tttcgtccac cggagtacaa cgggtgcctg ataagagaag aactgctaag
     3121 acttcatagc ttttgtgaaa aagccctttt tagtggatac gcctacggcg cttatttttt
     3181 ggtataacca tcaaaggcgc tttatggctc ctattgtctt gaaaatgtat ctttacttgt
     3241 attgtgtttt atgcgtagtc tccctgtgta gtatgtaact tgttaatttt catatgaata
     3301 cagaggcaca cgatgaaaaa cattgcagca atcaaacgta ataaccgcaa gattcacgct
     3361 cgtaagttcc tgtctacgcc agaaggaaaa gcctggctag agcgtaaaca gagagagaac
     3421 gaagaaagaa aactccttag tgagttgaaa tggcttagag aggatttctg aaaatctcca
     3481 gaacgcaaca ggacgcattc taacgctgtt ttcattactc ggtataggga taggtgatgt
     3541 gtgtttaatg cgtctgtgcg cttgttttgt gccttatttt gcattttcat aatgcggaag
     3601 agaacacgga aaaccttaaa attaattatt ggggtattta aggctatttt caggtgattt
     3661 aagggtatta aacttgatta aaatataaac agataattat gtatcttatt gatttataag
     3721 gttattttta agtaaaggta aataaaaaat aaatagccat aaatagctat aaatacatct
     3781 taatacctat atcccttccc ttatggctag attgcgagag gtgcttaacg cgattttcag
     3841 cgccccccga tctggaaaat ccagaatccc caaatatcag caaggaattt tcttgcgtaa
     3901 tcaacatcgt ttttacctat taaatcatta cacagaaagc ctttctgtaa tgtctctcac
     3961 taaactttca ctaaacacga gaatgatcgg ttttatgtcg gatcagatgc tagaaacagc
     4021 accacgtctc acaagggctg taagcgatga aaccagtgtt tatgcgggtg caggtcaaaa
     4081 tataggccaa aatccactta acataataaa tgttatatgt gctaaggaac acatataacg
     4141 ccttgaattg atgtatcagg ggaaaagtga tggatttttt acgtaggagc acgaaagggc
     4201 ttctattgac ttttgtatgc tatgaaatat aattaaagtt ttttgttttt attgtgtgat
     4261 gtggtgggtt acacagggga atagattaaa tggtgttctg tttatacagg tttatttgtt
     4321 agatcaacaa tttggcattt ctaaacagat gtttgaacgt tgccaaatgt cttgacaaca
     4381 caagaaaagg gcttgctatg ccaggcatta cacgaaataa ggctataaaa acaaaataaa
     4441 ttttaaaatt tgcacaaacg agaaactcgt tcagaaacaa gcagaacaat ttttagccaa
     4501 caatttctga tggtgctctg taagccgcat tacacaaggc tttgcgaaag tgatgtaaaa
     4561 gtgaagaaag taagaattga acttttaagc tacttttcat tggatttttt agtagggaat
     4621 tacacaggga atagagagca ttattctatg atttacacag ggaatatagg gggattatgg
     4681 cacacacata gaaagacatt acacagagtg ctatgtgaca aatggatgta acgagaggtt
     4741 acattcattt atctgaaatg ctctctgtaa gaaagttggt agtaacgata tgttacatag
     4801 actaattatt gaatgtaacg atattacaca tcggtagtaa cgatacgtta catcggaaga
     4861 tgattggatg taacggtagc tcacagtgaa ctaggggtag gtcatagtga actaccccca
     4921 cagaattctg ttacccctat caagtctgat acccctatca aaactgatac ccgataagta
     4981 taaata
//
LOCUS       DAZPLI010000227         4911 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZPLI010000227 DAZPLI010000000
VERSION     DAZPLI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.229"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(148..3369)
                     /gene="carB"
                     /locus_tag="ACHUU3_004032"
     CDS             complement(148..3369)
                     /gene="carB"
                     /locus_tag="ACHUU3_004032"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR2083967.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3387..4535)
                     /gene="carA"
                     /locus_tag="ACHUU3_004033"
     CDS             complement(3387..4535)
                     /gene="carA"
                     /locus_tag="ACHUU3_004033"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR2083968.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         1114 a         1363 c         1253 g         1181 t
ORIGIN      
        1 agtgtgagct aaatcctgct tatgtcatca aaaatatcga ttccccaaaa gatttactta
       61 atgggcaaaa aaaacggtcc tcatcagaga accgtattac ttattcgatc aaattagcgg
      121 atgaaaaata tccgccatga cacgctatta tttgatctgc gcgtgcattt cctgcaccga
      181 aattactttt tcggtcgcgt cggcattcag cgccatcgcg gtagcgaaac caccgttcag
      241 ggtggtgtca taatgcactt tatattgcag cgcactgcga cggatcacgc gggagtcttc
      301 aatcgcgcga cggcctgaag tggtgttgat gatgtaggta tattcgccat tcttgatgcg
      361 gtcctgaatg tgcggacggc cttcatgcac cttgttcacc agacgcggat taatgcccgc
      421 ttcgcccagc acaatcgccg tgccgtgggt cgcatccagc tcgaagccct gtttcagcag
      481 ttttgccgcc aggtccacca cgcgttcttt atcgccttcg cgcacggaaa gcaacgcacg
      541 accgtgtttc ttcatggtgg agttgctgcc cagctgcgct ttggcaaacg cttcagcgaa
      601 ggtgcggccc acgcccatca cttccccggt ggagcgcatt tctggcccta acagcgggtc
      661 aacacccggg aatttattga acggcagcac cacttctttc accgagtagt acggcgggat
      721 aacttctttg gttacgccct gctcagccag cgatttgcca gccatcacac gcgccgccac
      781 ttttgccagc ggtacgccgg tggctttgga gacgaacgga acggtacgcg ccgcacgcgg
      841 gttaacttca atcaggtaga cttcgttgtt tttcaccgca aactgcacgt tcatcagacc
      901 gcgcacctgc aattcgaagg ccagtttctg cacctgctgg cgcatcacat cctgaatttc
      961 ctgacttaag gtgtaggctg gcagagaaca tgcggagtca ccggagtgca cgcccgcctg
     1021 ctcgatgtgc tccatgatgc cgccaatcag caccatttcg ccgtcgcaga tggcatccac
     1081 gtcaacttct accgcgtcat caaggaagtg atccagcaac actggcgcat cgttagacac
     1141 gctgaccgcc gtctggaagt agcgacgcag gtcagcttcg tcatagacga tttccatcgc
     1201 ccgaccgccg agaacgtaag acggacgtac caccagtggg tagccaatct ctttcgcctt
     1261 ctctaccgcc atttcaatag cggtaacggt ggcgttcgcc ggttgtttca gtttcagacg
     1321 ctcaaccgca tgctggaagc gttcacggtc ttctgcacgg tcgatagcat ccgggctggt
     1381 gccgataacc ggtacgccag cagcttccag cgcgcgcgcc agtttcagcg gggtctgacc
     1441 gccgtactga acgataacgc ctttcggctt ctcaatacgc acgatttcca gcacatcttc
     1501 cagagttacc ggctcgaagt agaggcgatc ggaagtgtcg tagtcggtgg agacggtttc
     1561 cgggttacag ttaaccataa tggtttcgta accgtcttcg cgcagcgcca gcgaggcgtg
     1621 tacacagcag tagtcgaatt cgataccctg accgatacgg ttcgggccgc cgccgagaac
     1681 catgattttt tcacggtcgg tagacggatt cgcttcgcac tcttcttcat aagtggagta
     1741 catgtaagcg gtgtcggtgg cgaactctgc cgcacaggta tccacgcgct tataaaccgg
     1801 gtgcaggtca tactggtcgc gcagcttgcg gatttccgct tcgcgcacgc ccgccagttt
     1861 tgccagacgc gcatcggcaa agcctttgcg tttcagctgg cgcaggaatt cagcgttcag
     1921 gccagtgatg cccacttccg ctactttctc ttccagacgc accagttctt caatctgtac
     1981 caggaaccag cggtcaatat tggtcaggtt gaacacgccg tccacagaca ggcccgcacg
     2041 gaaggcatcg gcgatgtacc agatacgctc tgcgccagca tctttcagtt cgcgacggat
     2101 tttggttaac gcttccgggt catcgaggct cactttcggg tcgaatccag tcgcaccgac
     2161 ttccaggccg cgcagcgctt tttgcaggga ttcctgctgc gtgcgaccaa tcgccatcac
     2221 ttcgccaacc gatttcatct gagtggtcag acggtcgtta gcaccagcga atttttcgaa
     2281 gttgaagcga ggaattttag taaccacata gtcgatggac ggctcgaagg aggccggagt
     2341 acgtccgcca gtgatgtcgt tcatcagttc gtcgagggtg taacccaccg ccagtttcgc
     2401 cgccacttta gcaatcggga aaccggtcgc tttcgacgcc agcgccgaag aacgggacac
     2461 gcgtgggttc atttcgataa caatcaggcg accgtttttc gggttcaccg caaactggac
     2521 attggaacca ccggtttcaa cgccgatttc acgcagcacc gccatcgagg cgttacgcat
     2581 gatttgatat tctttgtcgg tcagcgtttg ggctggcgcg acagtgatgg agtcaccggt
     2641 atggatgccc atcgcatcaa agttttcgat agagcagacg atgatgcagt tgtcgttttt
     2701 atcacgcacc acttccatct cgtactcttt ccagccgatc agcgactcat caatcagcag
     2761 ctctttggtt ggggagagat ccagaccgcg ggcgcaaatt tcttcaaact cttcgcggtt
     2821 ataagcgata ccgccgccgc taccgcccat ggtaaaggat gggcgaataa tgcacgggaa
     2881 gcccacgtca gcggcaaccg ccagcgcttc ttccatcgtg tgtgcgatac cggaacgcgc
     2941 ggtttccaga ccaattttct tcatcgctac gtcgaaacga cggcggtctt ctgctttatc
     3001 aatcgcatcg gcggtcgcac caatcatagt cacgccgaac tcttccaaca cgccctgacg
     3061 ctccagctcc agcgcgcagt tcagcgccgt ctgaccgccc atcgttggca gcaccgcgtc
     3121 cgggcgctct ttttcaataa tcttgcgtac tacttcccag tgaatcggct cgatgtaggt
     3181 cgcatcggcc atttccgggt cggtcatgat ggtcgccggg ttggagttca ccagaatgac
     3241 gcggtaaccc tcttcgcgca gggctttaca tgcttgcgcg ccagagtagt caaactcaca
     3301 cgcctgaccg ataacaatcg ggcccgcacc cagaatcagg atacttttta tatctgtacg
     3361 ttttggcatg gctcttttac tcctgattac ttagcggttt tacggtactg ctcaattaac
     3421 tcgataaagt ggtcgaacaa cggcgctgcg tcgtgtggcc cagggctggc ttccgggtga
     3481 ccctggaagc tgaacgccgg tttatcagtg cgatgaatgc cctgtaacgt accgtcgaac
     3541 agggatttat gcgtgacacg caggtttgca ggtaatgttg cttcatccac cgcaaaaccg
     3601 tggttctggg cggtgatcat caccacgttt ttctccacat ctttaaccgg atggttgccg
     3661 ccgtggtgac caaatttcat tttgacagtc ttcgcaccgc tcgccagcgc cagcagctga
     3721 tgaccgaggc agatgccgaa taccggaata tcggtttcga ggaatttctg gatggcggta
     3781 atggcgtaat cgcacggggc cgggtcgcca ggaccgttgg agaggaagat gccgtctgga
     3841 ttcattttca gcacatcttc cgcagaagtt tgcgccggaa cgatggtcag gcgacagcct
     3901 ctatccacca gcatccgcag gatgttgcgc ttggcaccaa aatcataagc cacgacgtgg
     3961 aacggcagct cgtcttcttt tttcgcttct ggcaggccgc cggtcaacgt ccagctccct
     4021 tgtgtccagc tataggcttc tgcggtggtc acttcttttg ccagatccat gccattcaga
     4081 cctgggaacg cgcgggcttt ttctaacgcc aacgccgcat ccgggttatc gcctgcgata
     4141 atgcagccat tctgtgcgcc tttctcgcgc agtaaacgcg tcagcttacg ggtatcgata
     4201 tcggcaatcg ccacgatgtt atggcgcttc aggtaagaag agaggtcttc ggtattacgg
     4261 aagttgctgg caatcagcgg cagatcgcga atcaccagac cttgtgcatg tacctgagaa
     4321 gattcttcat cggcgtcatt agtgccgaca ttgccaatat ggggataagt aagagtaacg
     4381 atttgacgag aataggaagg atcagtgagg atttcttgat aaccggtcat tgaagtattg
     4441 aaaacgactt ccccaaccgc cgaacctgtt gcccctatgg cccgaccgtg aaactgggtt
     4501 ccgtcttcca gaaccaatag cgctgactta atcaaaacac cctccagaga atattcactc
     4561 actttatttg catattaatt cacaatgatg acgtaaatca atgcaaatct gcttaccgac
     4621 gaatttctgg caaacggcgg cattctggag atatgggcgc taaaagtcaa cttaatggcg
     4681 gtatttttta tattattttg tggctcatgc atgttttaca ggcattaagc gacaaaaaga
     4741 tcatataacc tgttctggaa aacgcttgcg cagcaagaag attgcatgaa ataaaaacga
     4801 gcaggaaaaa gtggaccaaa tggtcaaaat ttacattaaa acaacaaaaa cagaagacaa
     4861 aacatgtaat ttttggcact tatagaatat ttaaaaacat aacactaaaa a
//
LOCUS       DAZPLI010000228         4844 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZPLI010000228 DAZPLI010000000
VERSION     DAZPLI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.230"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(34..954)
                     /locus_tag="ACHUU3_004034"
     CDS             complement(34..954)
                     /locus_tag="ACHUU3_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR2083969.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1092..1991
                     /gene="pgrR"
                     /locus_tag="ACHUU3_004035"
     CDS             1092..1991
                     /gene="pgrR"
                     /locus_tag="ACHUU3_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR2083970.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2328..3941
                     /gene="mppA"
                     /locus_tag="ACHUU3_004036"
     CDS             2328..3941
                     /gene="mppA"
                     /locus_tag="ACHUU3_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR2083971.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(3992..>4844)
                     /gene="ynaI"
                     /locus_tag="ACHUU3_004037"
     CDS             complement(3992..>4844)
                     /gene="ynaI"
                     /locus_tag="ACHUU3_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR2083972.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT         1307 a         1081 c         1140 g         1316 t
ORIGIN      
        1 ccatatcact attgcggatt tgaatatgtt tcattacaga gtttcctcaa agaacggagc
       61 taatactgaa attgcttcag ccacataggc tttgccgtca taaagatcca tatggtttgc
      121 gccttcaaca atgtgatagc gtttatcttg gcttgatgct cgatcgtaca ggtcgtcact
      181 catccatttg ctccctgcct ggctgcccgc cacaatctgc atcggctgag tcaggtacac
      241 ttccgccata tggtaagcat caaaggtaat aatctggtta aggctgcgca aagtagcgta
      301 acccggtgct gttggatact gcgcgcgagg ggtgtggtaa tactcccagg cctgacgcag
      361 ttcttcgttc ggcgcatcgg actccttcat tggtgccagt ggcataacgg catattctcc
      421 gctgctaata tcgctggttc gggcgtttga acccgcttca acgtatggca gggcatcaat
      481 agatttcaca ttgttttccc aaccattacg gaacatcgaa ccaatattga ccgcactaac
      541 ggtaccgatg gccttgatgc ggcgatcctg aattgcagca ttggctgtat atcctgcacc
      601 ggcacaaatt cccatcgcac caattcgggt attgtcgaca taagaaagcg ttgtcaggta
      661 atcaatcacg gcactgatgt cttcagtacg aatgtatggg ttttctaact gacgcggctc
      721 gccgccactt tcaccttgat aagatgcgtc ataagcaata gtgacaaatc ccttttccgc
      781 cagttttttg gcataggttc cggccgtttg ttctttaacg cccccacctg ggtgagatac
      841 cacaattgcc tgatactgac gggtttcatc aaattttgga gggaaataga tcactgcaga
      901 caaagagatg gttggattat tgctgttagt gaagctgact ttattattca tcattccgtt
      961 cctcatggag ttgtcggttc gttttaacgg ttggtgatat cactatagat attgatcatt
     1021 aagttgatta gaccctaaat tatgattaga cctatcattt aaatgattaa tagattgcat
     1081 ggagatactg aatgaagcgt gaagaaatcg ctgatctgat ggcgtttgtc gtcgttgcag
     1141 aggagcgtag cttcactcgt gcagcagccc gcctgagcat ggcgcagtca gctttaagcc
     1201 agatagtgcg tcgtatagaa gaacgattgg gattgcggct tctgacgcga accacgcgca
     1261 gcgttgttcc aactgaagcg ggcgagcatc ttttgtctgt tcttggccct atgttgcatg
     1321 acatagattc agccctggca tccctgagcg agctgcagaa ccgcccatcc gggacaatac
     1381 gtattactac tgtagaacat gcagccaaaa cgatattgtt accagcaatg cgcacattcc
     1441 tgaaatcgca tcctgaaatt gatattcagc tcaccattga ttatggtttg accgatgtcg
     1501 tttctgaacg ttttgatgca ggcgtccgtc tgggtgggga gatggataaa gatatgatcg
     1561 ccattcgaat cgggccagat ataccaatgg ctattgttgg ctcaccggat tatttttctc
     1621 gccgaagtgt tccaacgtca gtgtcacaat taatagatca tcaggcaatt aatttgtatc
     1681 ttcccacatc gggtacagca aatcgctgga gattaatacg cggtggacgt gaagttcgtg
     1741 ttcgcatgga aggtcagctt ttactgaata cgatagacct gatcattgat gctgcaattg
     1801 atgggcatgg attggcgtat ctaccttatg atcaggttga gcgggctatt aaagaaaaaa
     1861 aactgatacg tgttttggat aaattcacac cagatttacc cggttatcac ctgtactatc
     1921 cacaccgtcg acatgctggc tcggcattct cattatttat agataggctg aagtataaag
     1981 gtgctgttta gcactacttg ctgatacatt aatttaattt ttctcttaac gtattctcag
     2041 ttcctttcaa cgttttggtt attttttatt cttcgtacaa tggcgacaga tgctgattat
     2101 gataccgaaa actggtttga acgtgcgaag cctgaacgta gtgttcggtg ttctatgtgc
     2161 tttaccgcat tttgtcgtct attatttaca ctaaatctga tttaatatat tgatacataa
     2221 aacattttat gctccctgtg tcactttttt gatggaagtt gtttgcattt ctttaaggcg
     2281 aaacaaataa ttacgcatca attttaatgt cggttagagg gaaacttatg aagcactctg
     2341 tttcagtcac gtgttgtgcg ctgttggtca gcagcatttc tctttcgtat gctgcagaag
     2401 ttccgagcgg cacagtactg gcagagaagc aggagctggt gcgccacatt aaagatgagc
     2461 ctgcgtcgct ggatcccgct aaagccgtgg gcctgccaga gattcaggtc attcgcgatc
     2521 tgtttgaagg actggtgaat cagaacgaga aaggggagat tgtccccggc gttgcgactc
     2581 agtggaaaag taatgacaac cgtatctgga cttttaccct gcgcgataac gcaaaatggg
     2641 cggatggcac accggtaacg gcgcaagatt ttgtctacag ctggcagcgt ctggtggacc
     2701 cgaaaacgct atcgccattt gcatggtttg ccgcgctggc gggaatcaac aacgcacagg
     2761 cgattattga tggtaaagct acgccagacc agcttggcgt caccgcagtt gatgcccata
     2821 ctttgaaaat tcagcttgat aaaccgttgc cgtggtttgt gaatttaacc gctaactttg
     2881 ccttcttccc ggtgcaaaaa gccaacgtag aaagcggtaa agagtggacg aaacccggaa
     2941 atctgatcgg caatggcgct tatgttctta aagatcgcgt agtcaatgaa aaactggtcg
     3001 tggtaccgaa tacccattat tgggataacg ccaaaacggt actgcaaaaa gtgaccttcc
     3061 tgccaattaa tcaggaatcc gcagccacta agcgttacct cgcgggggat attgatatca
     3121 ccgaatcctt cccgaaaaat atgtatcaga agctgttgaa ggatattccg gggcaggttt
     3181 atacgccgcc gcagctcggg acctattatt atgcgtttaa cacgcaaaaa gggccgacgg
     3241 cagatcagcg cgttcgtctg gcattaagta tgacgataga tcgccgcctg atgaccgaaa
     3301 aagtattagg gacgggcgaa aagccagcgt ggcattttac accagatgtt accgccggat
     3361 ttacgccgga accttcgccg tttgaacaaa tgagtcagga agaactgaat gcgcaggcaa
     3421 aaactttgtt gagcgcagct ggttatggtc cgcaaaaacc gctgaagctg acgcttttgt
     3481 ataacacttc agaaaaccat caaaaaattg cgattgctgt agcatcgatg tggaaaaaga
     3541 accttggcgt agatgttaaa ttgcaaaatc aggaatggaa aacctatatc gatagccgta
     3601 acaccggcaa ttttgatgtt atccgcgcct catgggtggg ggattataat gaaccctcca
     3661 ctttcctgac attattaacg tcaacgcatt caggaaatat ttcacgcttt aacaatccgg
     3721 catacgacaa agttctggcc caggcatcga cggaaaatac cgttaaagcg cgtaatgccg
     3781 attacaacgc ggcagaaaaa atcctcatgg agcaagcacc gattgcacca atttatcaat
     3841 ataccaatgg acgattaatc aagccgtggc tgaaaggtta tcccattaat aacccggaag
     3901 atgtggcgta cagccggact atgtatattg tgaagcattg atgtgatggg aactggcgtt
     3961 acccttgtgg ataacgccag tgatgtctga tttagcgccc ctgttccggc ggtgtaatgt
     4021 tatccatata cagcgtctgg ctggggaagg caaagtccgc gccgtgtgac tgcacaatat
     4081 cgataatctt caaataaacg tcttgctgtg cagcaagcca ttcagcccat accgtggttt
     4141 tggtaaagca ataaaccata atattcaatg acgagtcggc aaactggttg aaataaacca
     4201 gtaaggtttg tcgctggtcg atggccgggt gatttttcag catctcacgt acggcttcga
     4261 caataacgcc cacttttgcc gcatcctcat aacgtaaacc aatggtcgtg gtaatgcggc
     4321 ggttggtcat tcgtcctggg ttttctacgc tgatcgacga aaacagcgag ttcggtacgt
     4381 acaatggacg attatcaaag gtcgtaattt tggtaattcg ccagccaatt tccgctactg
     4441 taccttcgat atttctgtcc ggtgaacgga tccagtcgcc aatactgaaa ggacggtcga
     4501 aatagagcat gatcccggaa aagaaattac tcagaatatc tttaccggcc ataccgacag
     4561 ccagaccacc aataccacca aaggtcagca agccagaaag gctcatgccg aaatgttcgc
     4621 cataaagcag aacaagcacc acaataatgg tgattttgat aatgcgcgac ataatccgcg
     4681 cactggtgat atcgcgacct tttttaatct gctgtttttc aaactgatta atcagcaaaa
     4741 atagcttaat cgtcagaata accgcaatca gggacgtaca gataaaatcg ataacgtctg
     4801 gggtgataaa tttgagttta tagttttcta taacataatt aata
//
LOCUS       DAZPLI010000229         4834 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZPLI010000229 DAZPLI010000000
VERSION     DAZPLI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.231"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(108..551)
                     /locus_tag="ACHUU3_004038"
     CDS             complement(108..551)
                     /locus_tag="ACHUU3_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR2083973.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            708..1637
                     /gene="metA"
                     /locus_tag="ACHUU3_004039"
     CDS             708..1637
                     /gene="metA"
                     /locus_tag="ACHUU3_004039"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR2083974.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1906..3507
                     /gene="aceB"
                     /locus_tag="ACHUU3_004040"
                     /pseudo
     CDS             1906..3507
                     /gene="aceB"
                     /locus_tag="ACHUU3_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3537..4828
                     /gene="aceA"
                     /locus_tag="ACHUU3_004041"
                     /pseudo
     CDS             3537..4828
                     /gene="aceA"
                     /locus_tag="ACHUU3_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1206 a         1273 c         1225 g         1130 t
ORIGIN      
        1 gcagaaggcc atcctgacgg atggcctttt tgcattggcg cagaaaaaaa tgccgggtgc
       61 gacgctggcg cgtcttctcc agcctgggca ggcacggcag gggcagctta ttcctccaca
      121 tacgccagat tcagcaacgg atacggtttc cccaaatcgt ccacctcaga gcgtcccgta
      181 accttaaaac ccaccttctt atagaaccca accgcctgct cattttgctc attaacgttg
      241 gttgtcagtt ccggtgccat cgagagcgca tgctccacca gcatccgacc cacgccgcag
      301 ccgcacacat caggatcgat aaacagcgca tccatatgct gcccacttaa caacataaac
      361 ccaaccggct gatcccgttc attaaccgcg acccacaacg gcgcttccgg caggaaggaa
      421 cgaactagca cctccagctc ggcccgatac tctgctgata aaaaatcgtg tgtggcatcg
      481 acagaacgac accaaatcgc aacgagttcc tccccttcct catgccgtga gcggcgaata
      541 ctaataacca ttttctctcc ttttcgtcat tcttatattc taacgtagcc ttttccttga
      601 aactttctca ccttcaacat gcaggctcga cattggcaaa ttttctggtt atcttcagct
      661 atctggatgt ctaaacgtat aagcgtatgt agtgaggtaa tcaggttatg ccgattcgtg
      721 tgccggacga gctacccgcc gtcaatttct tgcgtgaaga aaacgtcttt gtgatgacaa
      781 cttctcgtgc gtctggtcag gaaattcgtc cacttaaggt tctgatcctt aacctgatgc
      841 cgaagaagat tgaaactgaa aatcagtttc tgcgcctgct ttcaaactca cctttgcagg
      901 tcgatattca gctgttgcgc atcgattccc gtgaatcgcg caacacgccc gcagagcatc
      961 tgaacaactt ctactgtaac tttgaagata ttcaggaaca aaactttgac ggtctgatag
     1021 ttaccggcgc gccgctgggc ctagtggagt ttaatgatgt cgcttactgg ccgcagatca
     1081 aacaggtgct ggagtggtcg aaagatcacg tcacctcgac gctgtttgtc tgctgggcgg
     1141 tacaggccgc gctcaatatc ctctacggca ttcctaagca aactcgcacc gacaaactct
     1201 ctggcgttta cgagcatcat attctccatc ctcatgcgct tctgacgcgt ggctttgatg
     1261 attcattcct ggcaccgcat tcgcgctatg ctgactttcc ggcagcgttg attcgtgatt
     1321 acaccgatct ggaaattctg gcagagacgg aagaagggga tgcatatctg tttgccagta
     1381 aagataagcg cattgccttt gtgacgggcc atcccgaata tgatgcgcaa acgctggcgc
     1441 aggaatattt ccgcgatgtg gaagccggac tagacccgga tgtaccgtat aactatttcc
     1501 cgcacaatga tccgcaaaac aaaccgcgcg cgagctggcg tagtcacggt aatttgctgt
     1561 ttaccaactg gctcaactat tacgtctacc agatcacgcc atacgatcta cggcacatga
     1621 atccaacgct ggattaatct tctgtgatag tcgatcgtta agcgattcag caccttacct
     1681 caggcacctt cgggtgcctt ttttatttcc gaaacgcacc tcagcaagtg aataaatttt
     1741 attcatattg ttatcaataa gttatcaagt atttttaatt aaaatggaaa ttgtttttga
     1801 ttttgcattt taaatgagta gtcttagttg tgctgaacga aaagcgcaca acgatccttc
     1861 gttcacagtg gggaagtttt cggatccatg acgaggagct gcacgatgac tgaacaggca
     1921 acaacaaccg atgaactggc tttcacaagg ccgtatggcg agcaggagaa gcaaattctt
     1981 actgccgaag cggtagaatt tctgactgag ctggtgacgc attttacgcc acaacgcaat
     2041 aaacttctgg cagcgcgcat tcagcagcag caagatattg ataacggaac gttgcctgat
     2101 tttatttcgg aaacagcttc cattcgcgat gctgattgga aaattcgcgg gattcctgcg
     2161 gacttagaag accgccgcgt agagataact ggcccggtag agcgcaagat ggtgatcaac
     2221 gcgctcaacg ccaatgtgaa agtctttatg gccgatttcg aagattcact ggccccggac
     2281 tggaacaaag tgatcgacgg gcaaattaac ctgcgtgatg cagttaacgg caccatcagc
     2341 tataccaatg aagcaggcaa aatttatcag ctcaagccca atccagcggt tttgatttgt
     2401 cgggtacgcg gtctacactt gccggaaaaa catgtcacct ggcgtggtga ggcaatcccc
     2461 ggtagcctgt ttgattttgc gctctatttc ttccacaact atcaggcact gttggcaaag
     2521 ggcagcggtc cctatttcta tctgccgaaa acccagtcct ggcaggaagc ggcctggtgg
     2581 agcgaagtct tcagctatgc agaagatcgc tttaatctgc cgcgcggcgc catcaaggcg
     2641 acgttgctga ttgaaacgct gcccgccgtg ttccagatgg acgaaatcct tcacgcgctg
     2701 cgcgaccata ttgttggtct gaactgcggt cgctgggatt acatcttcag ctatatcaaa
     2761 acgttgaaaa actatcccga tcgtgtcctg ccagacagac aggccgtgac gatggataaa
     2821 ccattccaga atgcttactc acgcctgctg attaaaacct gccataaacg cggtgctttt
     2881 gcgatgggcg gcatggcagc gtttattccg agcaaagatg aagagcgtaa taaccaggtg
     2941 ctcaacaaag taaaagcgga taaagcgctg gaagtcaata acggtcacga tggcacatgg
     3001 atcgctcacc caggccttgc ggacacggca atggcggtat tcaacgacat tctcggctcc
     3061 cgtaaaaatc agcttgaagt attgcgcgaa caagacgcgc cgattactgc cgatcagctg
     3121 ctggcacctt gtgacggtga acgcaccgaa gaaggtatgc gcgccaacat tcgcgtggct
     3181 gtgcagtaca tcgaagcgtg gatctccggc aacggctgcg tgccgattta tggcctgatg
     3241 gaagatgcgg cgacggctga aatttcccgt acctcgatct ggcagtggat ccatcatcaa
     3301 aaaacgttga gcaatggcaa accggtgacc aaagccttgt tccgctagat gctgggcgaa
     3361 gagatgaaag tcattgccag cgaactgggc gaagaacgtt tctcccaggg ccgttttgac
     3421 gatgccgcac gcttgatgga acagatcacc acttccgatg agttaattga tttcctgacc
     3481 ctgccaggct accgcctgtt agcgtaaacc accacataac tatggagcat ctgcacatga
     3541 aaacccgtac acaacaaatt gaagaattac agaaagagtg gactcaaccg cgttgggaag
     3601 gcattactcg cccatacagt gcggaagatg tggtgaaatt acgcggttca gtcaatcctg
     3661 aatgcacgct ggcgcaactg ggcgcagcga aaatgtggcg tctgctgcac ggtgagtcga
     3721 aaaaaggcta catcaacagc ctcggtgcac tgactggcgg tcaggcgctg caacaggcga
     3781 aagcgggtat tgaagcagtc tatctgtcgg gatggcaggt agcggcggac gctaacctgg
     3841 cggccagcat gtatccggat cagtcgctct atccggcaaa ctcggtgcca gctgtggtgg
     3901 agcggatcaa caacaccttc cgtcgtgccg atcagatcca atggtccgcg ggcattgagc
     3961 cgggcgatcc gcgctatgtc gattacttcc tgccgatcgt tgccgatgcg gaagccggtt
     4021 ttggcggtgt cctgaatgcc tttgaactga tgaaagcgat gattgaagcc ggtgcagcgg
     4081 cagttcactt cgaagatcag ctggcgtcag tgaagaaatg cggtcacatg ggcggcaaag
     4141 ttttggtgcc aactcaagaa gctattcaga aactggtcgc ggcgcgtctg gcagctgacg
     4201 tgacgggcgt tccaacccta ctggttgctc gtaccgatgc cgatgcagcg gatctgatca
     4261 cctccgattg cgacccgtat gacagcgaat ttattaccgg cgagcgtacc agtgaaggct
     4321 tcttccgtac tcatgcgggc attgagcaag cgatcagccg tggcctggcg tatgcgccat
     4381 atgctgacct ggtctggtgt gaaacctcca cgccggatct ggaactggcg cgtcgctttg
     4441 cacaagctat ccacgcgaaa tatccgggca aactgctggc ttataactgc tcgccgtcgt
     4501 tcaactggca gaaaaacctc gacgacaaaa ctattgccag cttccagcag cagctgtcgg
     4561 atatgggcta caagttccag ttcatcaccc tggcaggtat ccacagcatg tggttcaaca
     4621 tgtttgacct ggcaaacgcc tatgcccagg gcgagggtat gaagcactac gttgagaaag
     4681 tgcagcagcc ggaatttgcc gctatacctt cgtatctcac cagcaggaag tgggtacagg
     4741 ttacttcgat aaagtgacga ctattattca gggcggcacg tcttcagtca ccgcgctgac
     4801 cggctccact gaagaatcgc agttctaagc aacc
//
LOCUS       DAZPLI010000230         4803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZPLI010000230 DAZPLI010000000
VERSION     DAZPLI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.233"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            6..81
                     /locus_tag="ACHUU3_004042"
     tRNA            6..81
                     /locus_tag="ACHUU3_004042"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHUU3_004043"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHUU3_004043"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHUU3_004044"
     CDS             complement(299..1057)
                     /locus_tag="ACHUU3_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR2083975.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHUU3_004045"
     CDS             complement(1065..2168)
                     /locus_tag="ACHUU3_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR2083976.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="ACHUU3_004046"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="ACHUU3_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4459)
                     /locus_tag="ACHUU3_004047"
                     /pseudo
     CDS             complement(3427..4459)
                     /locus_tag="ACHUU3_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
BASE COUNT         1317 a         1241 c         1147 g         1098 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcattc agcatggcga
     3721 aaagcgtcca gcgtacaatc gagaaccatt catcatcgcc acgacgaact accggcccaa
     3781 gcggttcttt agagataacc tccggtaaga caatccattc agctgggttg cttaatttga
     3841 tgcgcagggc atacagttgc gactgatccg aggccagcgt atcgcagcga ccagattcca
     3901 gtgccttcgc tgattcgtca gagcgatcga aagtcaccgg tgtgtacttc atattgtttg
     3961 ccttgaaata gtcggcgacg ttgagttcgg tatctgtacc cgcctgaata cagacggtag
     4021 cgccatccag ttctttcgcg ctttttagcc ccgctttatc gtgcgtcagg aagccaatgc
     4081 cgtcgtaatc agtgacgcca gtaaatgcca ttcccatccc ggcatcgcga gatgaagtcc
     4141 aggtcgtatt acgggagagc aaatccacct cccctgactg taaagcggtg aagcgttctt
     4201 ttgcagtgag cggggtatat ttcactttcg tgtcgtcacc aaatacagca gcggcaacac
     4261 cacgacaaac atctacatca atacctgaaa acttaccgtc tgcatcggca taagagaacc
     4321 caggtaatcc atcactgatc ccgcattgca caaaaccttt tttctgtacg gcatcaagcg
     4381 tcgcgccagc atgcgcctga tttgcaacgg caagcagcac gctggcggca gccagtgtgg
     4441 ctatcatcat ctttttcata atgcttcctg tgaggcgaaa attatcgtta taggacgttt
     4501 gggaaacgtc ttttacctgt ctgtggccat cctcttataa gcaaaagtag tgccatcttt
     4561 tgcgcacgat ttcgcgtcgc aataagctga gtgtagacag gaaataataa aaacagcgcc
     4621 ccgcaatggt gcgaaaatga aacatgcgcc acattttggc gcaaaatatt tacgcgtgtt
     4681 ttactaagca gaataatgat tgttagcgtg agtatatttc tggtgggagg tgtgaaagtt
     4741 aaattgatgc gttatttcga aagcagcgaa ataacgcgat ataacatgct aaggtaatga
     4801 ctc
//
LOCUS       DAZPLI010000231         4766 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZPLI010000231 DAZPLI010000000
VERSION     DAZPLI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.234"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            21..542
                     /locus_tag="ACHUU3_004048"
     CDS             21..542
                     /locus_tag="ACHUU3_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR2083977.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQTQPEPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            598..831
                     /locus_tag="ACHUU3_004049"
     CDS             598..831
                     /locus_tag="ACHUU3_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFR2083978.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGAHFRLV
                     VRNNSGLVWRTWNFEEGAGYWMNHVIRNFGILK"
     gene            892..2850
                     /locus_tag="ACHUU3_004050"
     CDS             892..2850
                     /locus_tag="ACHUU3_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR2083979.1"
                     /translation="MSVAKSEPKSTRKASRKPAKTQETVLSALLAQTEEVSVPLDSLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERGILTADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALESDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDAADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDTLETAI
                     GFHMRDWWQPTKANFFGYLKKPQIIAALNDAGLSGAARDAERMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPRPQTDTTERTDNLADAA"
     gene            2905..3339
                     /gene="psiB"
                     /locus_tag="ACHUU3_004051"
     CDS             2905..3339
                     /gene="psiB"
                     /locus_tag="ACHUU3_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFR2083980.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFYLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFVPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            3336..4055
                     /locus_tag="ACHUU3_004052"
     CDS             3336..4055
                     /locus_tag="ACHUU3_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFR2083981.1"
                     /translation="MSVRSQALVPLSAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLTGMERWQWQDMPFWQVIAEAGMAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            4272..4424
                     /locus_tag="ACHUU3_004053"
                     /pseudo
     CDS             4272..4424
                     /locus_tag="ACHUU3_004053"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            4369..4491
                     /locus_tag="ACHUU3_004054"
     CDS             4369..4491
                     /locus_tag="ACHUU3_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR2083982.1"
                     /translation="MVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK"
BASE COUNT         1079 a         1236 c         1504 g          947 t
ORIGIN      
        1 tcaacagaag gagatacatc atggcagttc gtggcattaa caaggtcatt cttgtcggtc
       61 gtctgggaaa agatccggaa gtccgttaca tccccaacgg gggcgcagtg gcaaacctgc
      121 aggtggccac gtcagaaagc tggcgtgaca aacagacggg ggagatgcgg gagcagacag
      181 aatggcatcg cgtggtgctg ttcggcaagc tcgcggaagt ggcaggtgaa tatctgcgca
      241 agggcgcgca ggtctacatc gaggggcaac tccgaacccg tagctgggaa gataacggta
      301 ttacccgtta cgtcactgaa atccttgtga aaaccacggg caccatgcag atgctgggga
      361 gtgcaccaca gcagaacgct cagacgcaac cggagcctca gcagaatggt cagccacaga
      421 gtgctgacgc gacgaaaaaa ggtggcgcga aaacgaaagg ccgtggacgt aaggccgcgc
      481 agcccgagcc tcagcagccg tctgaacccg cgtatgactt cgatgacgac attccgttct
      541 aacgaaagac agtgacaacc gccccgtcca gtgcggggca tcaccggaga gatgaggatg
      601 agcgaatatt tcagaatatt tcaggggctg cctgacggct cctttacccg cgaacaggct
      661 gaagccgttg ccgcacagta ccggaatgtc tttatcgagg atgatcaggg agcgcatttt
      721 cgcctggttg tccgcaataa cagtggcctg gtctggcgta cctggaattt tgaggaaggt
      781 gccgggtact ggatgaacca tgtcatccgg aatttcggga tccttaagta agagaggtgc
      841 cggacgggga tcacgtccgg cagacattaa ctacacaata aggggatagt catgtcagta
      901 gcaaagtctg aaccaaaatc tacccgtaaa gcaagccgta aacctgcaaa aacgcaggaa
      961 actgtcctgt ctgccctgct ggcgcagacg gaggaagtga gcgtgccgct ggattcactg
     1021 attaaatcac cgctgaatgt gcgtacggtg ccgtattctg cggagtctgt cagcgaactg
     1081 gcggactcca ttaaaggtgt cggcctgtta cagaatctgg ttgttcatgc cctgcctggt
     1141 gaccgttacg gtgtcgccgc aggtggtcgc cgactggctg cactcaacat gctggcagag
     1201 cgtggcatcc ttacggctga ctggcctgta cgcgtgaagg tcattccgca ggagctggcg
     1261 actgccgcat cgatgaccga gaacggtcag cgtcgggata tgcaccctgc cgaacagatt
     1321 gccggattcc gcgcaatggc gcaggaaggc aaaacacctg cacaaatcgg tgatttgctg
     1381 ggctattcac cccgccacgt tcagcgaatg ctgaaactgg ctgaccttgc gcctgtcatc
     1441 ctcgatgcgc tggcagaaga ccgcatcacc accgagcact gtcaggcgct ggcgctggag
     1501 agcgacaccg cgcgtcaggt gcaggtgttt gaagctgcct gtcagtcggg atggggcgga
     1561 aaaccggatg tgcgggttat ccgcaatctg attacggaaa gtgaagtggc ggtggcgggg
     1621 aacagtaaat tccgcttcgt gggggctgat gccttctcgc cagacgaact gcgcaccgat
     1681 ttgttcagcg acgacgaggg tggctatgtg gactgcgtgg cgctcgatgc cgccctgctg
     1741 gaaaaactcc aggctgtcgc agagttcctt cgggaagccg aaggctggga atggtgcgcc
     1801 ggacgcatgg agcctgtcgg tgagtgccgt gaggatgccg gaacataccg ctgtctgccg
     1861 gagccggaag cggtgctgac cgaggcggaa gaagaacgtc tgaacgaact gatggcacgt
     1921 tacgacgcgc tggaaaacca gtgtgaggaa tctgacctgc tggaagcaga aatgaagctg
     1981 atgcgctgca tggcgaaggt cagagcgtgg acgccggaga tgcgtgccgg aggtggagtg
     2041 gtggtgtcct ggcgttatgg aaacgtatgt gtccagcgtg gtgtgcagtt acgcagtgag
     2101 gatgatgcgg ctgacgacgc tgaccgcacg gaacaggtgc aggaaaaagc gtcagtggag
     2161 gaaatcagtc tgccactgct gacgaaaatg tcttccgaac gcacgctggc agtccaggca
     2221 gcactgatgc agcaaccgga caaatccctg acactgctgg catggacgct ctgcctgaat
     2281 gtgtttggca gcggagcgta cagtaaacca gcacaaatca gcctggaatg taaacattat
     2341 tcgctgacca gcgatgcgcc atcggggaag gaaggtgccg cattcatggc gctgatggca
     2401 gaaaaagccc gtcttgctgc cctgttaccg gagggatggt cacgggacat gacgacattc
     2461 ctgtcactca gtcaggaggt gctgttatcc ctgctcagtt tctgcaccgc atgcagcctt
     2521 aacggtgtcc agacccgtga gtgtggtcac acgtcacgca gtccgcttga cacgctggaa
     2581 accgctatcg gattccacat gcgcgactgg tggcagccga caaaagcaaa cttcttcgga
     2641 tacctgaaaa agccgcagat tatcgcagct ctgaatgatg ccggactgtc cggtgccgca
     2701 cgggatgcag agaggatgaa gaaaggtgat gcggctgaac atgcagagca ccatatgaaa
     2761 gacaaccgct gggtgcctgg ctggatgtgt gcaccacgtc cacagacgga taccactgaa
     2821 cgcaccgata acctggctga tgccgcctga tgaacaacca caccgccccg ccggagacgg
     2881 ggcggcagca agggagatac cgtgatgaaa actgaactga ccctgaatgt attacagacc
     2941 atgagtgcac aggaatatga agatatccgg gctgccggaa gtgatgaacg ccgtgagctg
     3001 acacacgccg tgatgcggga actggatgcg ccggacaact ggacgatgaa tggcgagtac
     3061 ggcagcgagt tcggcggctt tttccccgtc caggtccgtt tcacgcctgc ccacgaacgt
     3121 ttttacctgg cgttatgttc accgggcgat gtctctcagg tctgggtgct ggttctggtg
     3181 aatgccggtg gtgagccgtt tgcggtggtt caggtgcagc ggcggtttgt accggaagcc
     3241 gtcagccatt cactggcact ggcggcgtca ctggatgcgc aggggtacag cgtcagtgac
     3301 atcatccata tcctgatggc ggaaggaggt caggcatgag cgtacgttca caggcactgg
     3361 ttcccctcag cgcagagcaa caggccgcat ggcgggcggt ggcggagacg gaaaaacgcc
     3421 gccatcaggg taacacgctg gcggaatatc cgtatgcggg ggcgtttttc cgctgtctga
     3481 acggaagccg caggatatcg ctgtctgacc tgcgattttt catgccgtcc ctgaccgcag
     3541 aagaactgca cggcaaccgt cttcagtggc tgtatgccat cgatgtgctg attgaaacac
     3601 agggcgaggt ctgcctcctt ccccttccag gcgacgcagc agaacgcttg ttcccgtcgg
     3661 tgcggtttcg tgtcagggaa cgcagtcgcc ataaatccgc gctggtcatg cagaagtaca
     3721 gccgccagca ggcgagggag gcagagcaga aagcccgtgc gtaccaggcg ctggtcgctc
     3781 aggctgaaat cgaactggca ttccattcgc cggaaacagt cggaagctgg catgcacgct
     3841 ggagtgaccg ggtggcagag catgacctgg aaaccctgtt ctggcagtgg ggcgagcgtt
     3901 ttccgtcact gaccgggatg gaacgctggc agtggcagga tatgccattc tggcaggtca
     3961 tcgcagaggc tggtatggcg gcaagggagg ccggtcatgc cgtccgtgag atggagcgat
     4021 ggatggtgcc gaacaaactc cgggaggcgg cgtgatgcgg caacaatcac acagattacc
     4081 cgtaaacagc ctgaatgagc gggttatttt cagggaaagt gagtgtggtc agtgcgcagg
     4141 tatatgggct atgatgtgcc cggcgcttga ggctttctgc ctcatgacgt gaaggtggtt
     4201 tgttaccgtg tcgtgtggca gaaggacaaa agccccgtag ttaatttttc attaacccac
     4261 gaggcctctg catgtctagt ccacgtcagt atggcctctt accgtgcaaa ttgcaaggag
     4321 gagtaaacca tgaaactacc gggaaacgcc cttatctggt gcgtattaat cgtgtgctgc
     4381 acgttgttga tattcacact cctgacccgg aatcgcctgt gcgaagtccg gctgaaggac
     4441 ggatacaggg aagttacggc aacaatggct tacgaatccg gcggtaagta gcaacctgga
     4501 ggcgggcgca ggcccgcctt ttcagggatg atgctggtct gactgcactg aagcgccttt
     4561 ataaaggggc tgctggttcg ccggtggccc ctttctcctt gctggcattg tgcgggcatg
     4621 aacaaccaga ctgccaggaa ggccgtttcc ctccgggaac ggggaagaac gcgcgcctgc
     4681 ggcgcccgtt cttttccccg ccctctctgt ttatggccag ttgtcagaaa tgacattacc
     4741 tttcccgtga ttattctctt tttctt
//
LOCUS       DAZPLI010000232         4748 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZPLI010000232 DAZPLI010000000
VERSION     DAZPLI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.235"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(71..352)
                     /locus_tag="ACHUU3_004055"
     CDS             complement(71..352)
                     /locus_tag="ACHUU3_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR2083983.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(597..1706)
                     /gene="apbC"
                     /locus_tag="ACHUU3_004056"
     CDS             complement(597..1706)
                     /gene="apbC"
                     /locus_tag="ACHUU3_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR2083984.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1838..3871
                     /gene="metG"
                     /locus_tag="ACHUU3_004057"
     CDS             1838..3871
                     /gene="metG"
                     /locus_tag="ACHUU3_004057"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR2083985.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4012..>4748
                     /locus_tag="ACHUU3_004058"
     CDS             4012..>4748
                     /locus_tag="ACHUU3_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR2083986.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQID"
BASE COUNT         1239 a         1188 c         1176 g         1145 t
ORIGIN      
        1 ataaaaaagc cgaaatcatt tatataaatg atttcggctt tctaaatgca attaaaatca
       61 ggttgccaaa ttaatcgtag cgatttaaca cgattcgacg tttgctatcg ctacctgcaa
      121 caaccacttc tgtcgttaca cctttgacgg gcgaagaaaa cagatgacgg gtgtcaacat
      181 tatacgttaa ctgaccacaa tgcggttgag gttgaccatt aaccatagcc atactgcatg
      241 ttgggggact gatcctgcca ttaatcgcta atgtggcagt ctctgaagag aatgcgggag
      301 aagccaatcc ataggcaaga aaaataattc ctgacagcca atacttattc attgaaggtt
      361 atgcctggat agaatgagtg cataacaaac tatagctgta catccactac acagccacga
      421 aggatgataa tgaagcattg cctgtatgat caatcgactt tgtagagttt cggacgaagg
      481 tccgcagaat attcgcagta ttaaataagt gttcattaat gcggcaaaaa ctaatacacc
      541 gcatcaatgt aacatctctg cggtgtatga agaataacca gaatggttaa taggcgttag
      601 accgcgcgga aggaaatctc gcctggaatg acttcaccct gccagtagag ctgagctgca
      661 acgcggtcag ccagctggcg gtagatagcg gtaaattcgc tctccggacg gctgataacc
      721 gttggcgtgc ctttatcgag atcctcacgt aaggagatgt gtaacggcat ctgccccagc
      781 agctgtgtgt ggtatttttc agccagtttc tctgcaccac cggtaccgaa aatcggttca
      841 tggtgaccac agttactgca aatatgcaca ctcatgttct cgacaatacc aagcaccgga
      901 acctcgactt tctcgaacat cacaatgcct tttttcgcat cgatcaaagc gatgtcctgc
      961 ggggtggtga ccacgactgc accagttact ggaatgtttt gcgccagcgt caattgaatg
     1021 tcaccggtgc ctggcggcat atcaagcacc agatagtcga gatccggcca caaggtttcc
     1081 tgcaacatct gcattaacgc cttgctcgcc atcggtccac gccacaccat cgcattgtcg
     1141 tcagtgacca gataaccaat tgaattggtt gccaggccat gagacataat cggtgccata
     1201 tgggtaccgt ccggcgaagt tggacgctga ttttctgcgc ccagcatggt gggaattgat
     1261 gggccgtaga tatcggcgtc cagaatgccg actttcgccc cttccgccgc cagtgccagc
     1321 gccaggttta ccgccgtgga ggatttaccc acgccgcctt taccggaact gaccgcaatg
     1381 atgttcttca caccattaat acccggctga ttcttcacgc gttttaaggt ggcgatattg
     1441 tgcgacagtt tccagtcgat agctttcgcc ccggtgatac gcagcagttc ggcgctacat
     1501 tgctctttta actcttcaaa cgcgctatgc cagacgaatg gcataaccag ttccacatgc
     1561 aacgtgtcgt ccatccaggc gacatggtgc aacgcgttca gcgtggtgag gttatgcttc
     1621 agggttgggt gctgaaaatt ggccagtgtc ccggcgacca ttgcgcgcaa ggcttctggt
     1681 gatttggcct gggattgttc gttcatcccg actccttttt tgtatagata aaccatcagc
     1741 tgatagttta cctgaagaat atagagaagt acttacttaa cattttccca tttggtacta
     1801 tctaacccct tttcactatt aagaagtaat gcctactatg actcaagtcg cgaagaaaat
     1861 tctggtgacg tgcgcactgc cgtacgctaa cggctcaatc cacctcggcc atatgctgga
     1921 gcacatccag gctgatgtct gggtccgtta ccagcgaatg cgcggccacg aggtcaactt
     1981 catctgcgcc gacgatgccc acggtacacc gatcatgctg aaagctcagc agcttggtat
     2041 caccccggag cagatgattg gcgaaatgag tcaggagcat cagactgatt tcgcaggctt
     2101 taacatcagc tatgacaact atcactcgac gcacagcgaa gagaaccgcc agttgtcaga
     2161 acttatctac tctcgcctga aagagaacgg ttttattaaa aaccgcacca tctctcagct
     2221 gtacgatccg gaaaaaggca tgttcctgcc ggaccgtttt gtgaaaggca cctgcccgaa
     2281 atgtaaatcc ccggatcaat acggcgataa ctgcgaagtc tgcggcgcga cctacagccc
     2341 gactgaattg atcgagccga aatcggtggt ttctggcgct acgccggtaa tgcgtgattc
     2401 tgaacacttc ttctttgatc tgccctcttt cagcgaaatg ttgcaggcat ggacccgcag
     2461 cggtgcgttg caggagcagg tggcaaataa aatgcaggag tggtttgaat ccggcctgca
     2521 acagtgggat atctcccgcg acgcgccata ctttggtttt gaaattccga acgcgccggg
     2581 caaatatttc tacgtctggc tggacgcacc gattggctac atgggttctt tcaagaatct
     2641 gtgcgacaag cgcagcgaca gcgtaagctt cgatgaatac tggaagaaag actccaccgc
     2701 cgagctgtac cacttcatcg gtaaagatat tgtttacttc cacagcctgt tctggcctgc
     2761 catgctggaa ggcagcaact tccgcaagcc gaccaacctg tttgttcatg gctatgtgac
     2821 ggtgaacggc gcaaagatgt ccaagtctcg cggcaccttt attaaagcca gcacctggct
     2881 gaatcatttt gacgctgaca gcctgcgtta ctactacact gcgaaactct cttcgcgcat
     2941 tgatgatatc gatctcaacc tggaggattt cgttcagcgt gtgaatgccg atatcgtgaa
     3001 caaagtggtt aacctggcct ctcgtaatgc gggctttatc aacaagcgtt ttgacggcgt
     3061 gctggcaagc gaactggctg acccgcagtt gtacaaaacc ttcactgatg ccgctgaagt
     3121 gattggtgaa gcgtgggaaa gccgcgaatt tggtaaagca gtgcgcgaaa tcatggcgct
     3181 ggctgatctg gctaaccgct atgtcgacga gcaggctccg tgggtggtgg caaaacagga
     3241 aggccgcgat gccgacctgc aggcaatttg ctcaatgggc ataaacctgt tccgcgtgct
     3301 gatgacttac ctgaagccgg tactgccgaa actgaccgag cgcgcagaag cattcctcaa
     3361 tacagaactg acctgggatg gtatccagca accgctgctg ggccacaaag tgaatccgtt
     3421 caaagcgctg tataaccgca tcgatatgaa gcaggttgaa gcactggtgg aagcgtctaa
     3481 agaagaagtg aaagcagctg ccgcgccagt tactggccca ctggcagatg atccgattca
     3541 ggaaaccatc acctttgacg acttcgcaaa agttgacctg cgcgtggcgc tgatcgaaaa
     3601 cgcagagttt gttgaaggtt ctgacaaact gctgcgcctg acactggatc tcggcggtga
     3661 aaaacgcaat gtcttctccg gtattcgttc tgcctacccg gatccgcagg cactgatcgg
     3721 gcgtcacacc attatggtgg ccaacctggc accgcgtaaa atgcgctttg gtatctccga
     3781 aggcatggta atggctgctg gtcctggcgg gaaagatatc ttcctgttaa gcccggatgc
     3841 tggtgctaaa ccaggccatc aggtgaaata atcccccttc aaggcgctgc atcgacagcg
     3901 ccttttcttt ataaattcct aaagttgttt tcttgcgatt ttgtctctct ctaacccgca
     3961 taaatactgg tagcatctgc attcaactgg ataaaattac agggatgcag aatgagacac
     4021 tttatctatc aggacgaaaa atcacataaa ttctgggcag ttgagcaaca gggaaacgag
     4081 ttgcatatca gttggggaaa agttggcacc aaagggcaaa gccagataaa aagtttttca
     4141 gatgctgcgg cagcggcaaa agcggagctt aagctgattg cggagaaggt gaagaagggg
     4201 tatgtggagc aagcgaagga taattctttg caaccttccc aaacggtaac gggctctctc
     4261 aaggtagcgg acttatccac cattattcag gaacaaccct ctttcgtagc agaaacccgt
     4321 gcgcctgaca aaaatacaga tgctgtttta ccgtggctgg cgaaagatat tgctgtcgtt
     4381 tttccgcccg aagttgtaca caccacgtta agtcatcgcc gctttcccgg agttcctgtt
     4441 cagcaagcag acaaattgac ccaattacgt cgcttagcct gtagtgtgtc gcaacgggat
     4501 aataaaacag ccacatttga cttcagcgcc tgttctttag aatggcaaaa caccgtcgcc
     4561 caggcgatca gtcagatcga cggcctgaaa acaacacagt taccatcacc agtaatggct
     4621 gtactcacgg cacttgaaat gaaatgcaca agatataaag tgcgtgagga tgttatggat
     4681 cagatcgtcc aggaaggcgg tctggaatat gctactgatg taataataca ccttcaacag
     4741 attgatat
//
LOCUS       DAZPLI010000233         4593 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZPLI010000233 DAZPLI010000000
VERSION     DAZPLI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.236"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..1380)
                     /locus_tag="ACHUU3_004059"
     CDS             complement(<1..1380)
                     /locus_tag="ACHUU3_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR2083987.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNN"
     gene            complement(1437..3809)
                     /locus_tag="ACHUU3_004060"
     CDS             complement(1437..3809)
                     /locus_tag="ACHUU3_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR2083988.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(3847..>4593)
                     /locus_tag="ACHUU3_004061"
     CDS             complement(3847..>4593)
                     /locus_tag="ACHUU3_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR2083989.1"
                     /translation="SRQAFMLVSNNLSWFIYKYDELAELAAVIDRLYEFHQLTEQRPT
                     NKPKNCQHAVQVANASIRTPDNKIILENLNFHVSPGKWLLLKGYSGAGKTTLLKTLSH
                     CWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICKALPLPVDDKSLSEVLHQVGLGKL
                     AARIHDHDRWGDILSSGEKQRIALARLILRRPKWIFLDETTSHLEEQEAIRLLRLVRE
                     KLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         1170 a         1065 c         1037 g         1321 t
ORIGIN      
        1 attgtttacc atttgctccc aaaatgcgtc ctggttcttt cttaactgct gccatttttc
       61 cgccagactt tgcacggtaa tttgctgcca cagacgtttc gaaagttgat atgtctcttc
      121 cggcgacaag aacggcgtat tatttaatga gctggatgcc aggcgactgg tcagcatacg
      181 taaatcacgt tcagcttgct gatcaaccgc atttttcagc caggtgaggc gggtagattt
      241 gacatcatcg agttcttcag cagaaaaacc atgctgatca atggttgcca actctgccat
      301 taatgcattt gcagcatcct gcatattatc gtctcgtgca tttacacgga aaaacagcga
      361 ctgataatcg ggtgcaattt taacgctgcg cgcagtgccg ccagaaatag tcttcaactc
      421 gcccgactgt atgcgttcct gcagacgttg attgaacagc tgaactaaca tgctccattc
      481 agcttgttcg acaaagcttt gctcatcgtt cacttgtacc attggcaggc gataatagag
      541 tgcgatgccg ttcactcggt tttctttatc attaatgata ttaaagcgca ggtggttttc
      601 ggcttttgtc ggccagacgc gattttcagc tgctttgtta gccggaagct tacttaaatt
      661 atcctttatc agcgccagcg cttctttact gtcgatatcg ccgaccacga taaaggtcat
      721 attatttggt tgataccagc gttgataaaa ttggcgcaat tgtgccggtg tgaccgtggc
      781 gacagtatcc atcaggccga taggttcacg gtctaaatta cgggtatttg ccagcaggaa
      841 agggcggcgc gcctgagagg tgcgccattt cgcatcctga tgggcacgcc attcctcagt
      901 aattacgccg cgttcagcgt ctacttcgag tatttcaaag gttgcggcat tactccattc
      961 actgaagatt gccatcactt gttgcagatt ttgtttctgc gtagtcggca gactcacctg
     1021 atacagcgtt tcgtcatagc tggtataggc attaacatcg cgaccgaaac gcaggcccat
     1081 tgactcaaat gtttcgatga ctttattacc cggccatgtt tttgtgccgt taaacatcat
     1141 atgttctaca aaatgagcca cgccgcgctc attgtcttct tcctgcaatg aaccggtatg
     1201 aatttgcagc cataaattta cctgatcctt tggttgagca tgcggataaa tcatatatcg
     1261 caagccattg tccagttgcc cggtaattaa cttttcatcc tgcggtaagg cggcggcaat
     1321 cagccgcccg gggagcaaca gagttgccac taacgtcagt aagaaacaga ggtttctcat
     1381 aattatctcc atgcgaaaac cgggcgaatt tacccggtta agtaaaatcc gaactattaa
     1441 aatttcatgc tgacatcaag ccagaaagta cggccactgg cgtaggtcgc cacaccggtg
     1501 ttcgttgtat caacagcggt tttgctatcc agtacattga gaatatcggc actgatcgtc
     1561 aggttttgtt gtttcagaaa ttgtggcgtc caggacaagc gggtgtccca ggtcaggctg
     1621 ctatccagtt tttcgtcaac atactgcttg taatctgaat attcgctgat gtattgcgca
     1681 tttgtcttac caaggataat gcgagcttta cgcgcttctt gccaggccag cgtattcgcc
     1741 cacactaacc cgctcggttg atgcgtgaaa tccatgttta aggagatctt taatgggtta
     1801 ttaaaatctg ccactggaac actatcgtaa gagaccagat taccgttata aaccacctgg
     1861 ttatcaccgg tattgctctc ctcataacca ttgtttaacg acaagttgcc cttgctcttg
     1921 atatagctaa agacaatttg tgggttaata tctacttggc ggatatgcag gggttcggcc
     1981 agttcaaaac tgaggctgaa cgaatgcgtt ttggttttgc catcgttgtt atattcagta
     2041 atggtggttt tagtcgcgct gtcggtacga ctgcttttgc tgatttgatc atgcgcttca
     2101 cggtaaacat agtttgcacg cgcaataacg ttcttaccga ttttctgctg caatcccatt
     2161 gccagttcat cgttataagg cgttttcaaa tcctgataac gcgtcagagt tttattacct
     2221 gataccgatt ccgtccagct attgcggata tcacgtaatc ccatatcaag aatattcccg
     2281 ccatagtaac ggttataacc ggcggtaatc attgaggttt gatcagcaaa aatatcccat
     2341 tccgtcataa agcgcgggga gatattgtgg tttgacagat agttgtcata gtcataacgc
     2401 acaccgggca ttaacgacac attccgccag ctaatgtgct ccgccatata cagcgtgtag
     2461 ttgtcaattc ccaggctgcc tttaccttta tggtaaatgg tatggttagt tttctttcct
     2521 gcagcattaa tcacatagga ttcagactgg ttatggcgtt cagtccatgc atcggaatag
     2581 atgtattccg cgccgaagta gggttgatgc gaaacatcac ccacggcgaa tttttgccag
     2641 tccaggcgtg ttttgaaggt gtaattatct acagcctggg aaatgtgtcc taatccgcca
     2701 caggtacaac gccctgtaat atcaccatat gtacatgaaa gttcggtgta ccagatgtca
     2761 tgatcgtgac gggtataatc actaatatga tcccaaccaa cggtggtacg tagtttggcc
     2821 catgcgagtt gcgtatccat atcccatgcc agaccatagg atttattacc catttcgcga
     2881 tcagactgcg ggaaggtgct ggtattataa tcacggctgg agccggtata ttttaaggtt
     2941 aaatcgtggg taaagcggtc gctggcaaac caggtaaatt tgctcaatgc agtatcgata
     3001 acgtttttat actgtgcccg accggtgaca atgccgtcat tcgaaacata atccgcgcgg
     3061 gtgatatcag actggcggcg cgataaaccg gcggtaacgc cgaagttatc agcgagttcc
     3121 tgattaaacg acaaggtata aaagttcttt ttaaaatctg gggagtaata agtacttcct
     3181 gaagagcctt gattaaatgc gctcttgtta ttctcatcga tatgcgatgt taaccagtcc
     3241 gaacgcgtag tgcgataacc caatttcacc ttgctatcat cagcgttgaa gcgtttgatc
     3301 tttgcatcaa ttaccccgcc attgaagcga ccaaattcaa ccggcacaaa actgtcataa
     3361 agcgtcacat tgtccagtaa gctgacatca agataatacc cctgtgacat cccgctaata
     3421 ttggttgcac tactggcatc ggactcattc gctgggttca ggttattagt tgcactaata
     3481 ccgtcaatca aataggcatt ctggtagggc gacgcaccgt gaatagagat tttctcaggg
     3541 cgaatatctc cctggttcaa cgaggtactt tgcgttgaat ccatgcgtac cgcagggttg
     3601 gttctcagca gatcgctgat attgccattc ccggtcggta aacgctcgat gctttcactg
     3661 gtgtaatgcg tgttgccgtt tacgggtaca gggacaggag catagaccgt catctcttca
     3721 aaaaaatgca tgtacatgtt tttgtcatcg acggcctgcg ccacatcagc gccacataaa
     3781 atgacgccag gaataagaac tcgcttcatt tatttatatc tccgtacagc agggctttta
     3841 ttgttgttat aaaaccgtgc taatgtcaca aatatcatca gccaggttcc agacaccggg
     3901 ttgatgtgta accataatga cgccgcttgt gggtagtttt tcacgcacta aacgcagtaa
     3961 gcgtatagcc tcttgttcct caagatgaga ggtagtttcg tcaagaaata tccatttcgg
     4021 acgtcgtaaa attaatcgtg ccagggcgat acgttgtttt tcgccgctgg aaagaatatc
     4081 tccccagcga tcgtggtcat gaatacgcgc agccaatttc cccagaccaa cctgatgcag
     4141 tacttcgctc aacgatttat cgtctacggg caggggaagt gctttacaaa taatctcttt
     4201 cagtaagccg cttttgatta acggtgtttg tgacacatac caactgtcag caggagaaga
     4261 aatatcacct ttaaaccacg gccagcagtg ggataatgtt ttaagcagtg tggtttttcc
     4321 cgcaccagag tagcctttca gtaatagcca tttgcctggc gaaacatgaa agttcaagtt
     4381 ctctaatatg atcttattat caggcgtacg aatactcgca ttagccactt gtaccgcatg
     4441 ttggcaattt ttaggcttat tcgtagggcg ctgttcagtg agttgatgga actcatacaa
     4501 gcgatcgata accgcagcca gttcagcaag ttcgtcatat ttataaataa accagcttaa
     4561 attgttcgat actagcataa atgcctggcg cga
//
LOCUS       DAZPLI010000234         4509 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZPLI010000234 DAZPLI010000000
VERSION     DAZPLI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.237"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..461
                     /locus_tag="ACHUU3_004062"
     CDS             <1..461
                     /locus_tag="ACHUU3_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFR2083990.1"
                     /translation="QNGLAKALREIGRIERTLFMLDWFRDPALRRRVQAGLNKGEARN
                     ALARAVFMHRLGEIRDRKPENQSYRASGLTLLTAAISLWNTVYMERAVDALKRKGMKI
                     NAQLLSHLSPLGWEHINLTGDYIWKNSRIPTSGKFRRLRSVKIDKVKKQP"
     gene            complement(987..2162)
                     /gene="senB"
                     /locus_tag="ACHUU3_004063"
     CDS             complement(987..2162)
                     /gene="senB"
                     /locus_tag="ACHUU3_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFR2083991.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(2231..4492)
                     /locus_tag="ACHUU3_004064"
     CDS             complement(2231..4492)
                     /locus_tag="ACHUU3_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR2083992.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
BASE COUNT         1146 a         1052 c         1049 g         1262 t
ORIGIN      
        1 aacaaaatgg gttggctaaa gctctgagag agattggccg tatagaacga acactgttta
       61 tgctggactg gtttcgggat ccggcattgc ggcgacgtgt ccaggccggg ctgaacaaag
      121 gagaagcccg taatgctctg gcccgtgctg tattcatgca tcgattgggg gagattagag
      181 atcggaaacc ggaaaatcaa agttatcgcg ccagtggatt gactttattg acggcggcta
      241 tttccttatg gaacacagtt tacatggaaa gagcggttga tgctctgaag cgtaagggca
      301 tgaaaataaa tgctcaacta ttatcgcatt tatcaccatt aggttgggaa catatcaatc
      361 tgacaggcga ttatatctgg aaaaacagcc ggataccgac ttcaggtaag tttcgtcgtt
      421 tgagatcagt taagatcgac aaggtcaaaa aacaacctta acgtacaata attttcgata
      481 tccaaactga ccccaagtat aaaaaacagc ctgtcacggt ataaaaatcg accatgcaga
      541 aattatgctg gttattttac cgctgctggt ccctcattca gtgactgccc caggggtttt
      601 catgctctcc ttatagtaca ataatttcca tatccaaact cacccctact ttgactttta
      661 tcatagtcaa aacctcttaa cgtgagtttt cgttccactg agcgtcagcc ctctaaaact
      721 actgaataat cagttagaaa acaaacaatc ggaaaaatct ggggttccgg cctagaaccc
      781 catgaacagg catagctgtg atttcattta attattggca caaatcagtt gatcgtacaa
      841 tgctgtgcct gtctcgtttt ctggagattc ctcagcctca tggagcggcg taacaggcgt
      901 accggcagga cagagatgtt tgcttttcct agtccggcca gtttagtgga ataacggagt
      961 ttttatccca tgttttttcc ccccgatcac gactttttca aatcaatgct cagaatacgt
     1021 tctttagctc cttccacaaa cacatcagga tcccaatcgc ggttaaagcc ttctttgatg
     1081 ccaatgtaaa gggtatccat atcgtccgac aacaccatct ggtttgggta gaccggcatg
     1141 ttaaattcac gggtgatgct gtatttggtg gcatccacta cgctgatggt gccgctagtg
     1201 aagttggtga catacagttc gttatatttc ggattgtatt tcaccgcgtt agctccggct
     1261 cctgtgtgca catagccaag ctgtttaccg tcgcgtaagt caaaaatata gagtccatct
     1321 tttgtaccgg aacgatcgtc atggtcgaat accaccacaa aggcacggtc agaagcggag
     1381 tccaccgcca gattctgcac acttgtgatt tgtggtctcg gatctttcaa cgggatggta
     1441 cgcagcagcg tctgattttt cgcatcaatt tcgttgatgt aatctcttcc gccaacgtag
     1501 attttctgtg ttttttcatc catcgtcaga ccatacgctg tacctttgta agcatcctta
     1561 acctcgcctt ttaaggcaag cgtcgtcccg tcgagcatta acagcttatg cagaggcttc
     1621 atgccctcgg ccgttttcag catattgcta taactcacaa acagggtatc ggaatcctgg
     1681 ctgtaaacca tatggcgaaa atgctcaaaa cgcgaatctg cagcgtttgc cgctttaaaa
     1741 cttaacctgt cgctggttct taccagtttg ccggttgggg tgtcaaacat actgatacgc
     1801 agggacgctg actgggtgtg tccgatatag agctgatgtt tatcctgatt tatcgccagc
     1861 gaaaatgccc ggtatggcat cgtgtattta ttttcgactt tcagcgaatc tgaattaaac
     1921 gaatacagat agcctttatt ggcttcacga tttacacgat cggtgactgc tgcaaacacc
     1981 tttttactgg cattatcaaa cgccagttca tagacgcctt catcagggga ctgaaggtaa
     2041 ttcgttttca gggcaaatcg cgaaagttcg cgttcaggtg gatcgatagc catggccatc
     2101 gtgggtaaaa atagtgaaat taacaggtat agcggtgctt tggataaagt gaaaatattc
     2161 atttttcgtt cctgcaataa aattgacata agagagaaaa cgtattgtct gaacctagcg
     2221 gaaattgtca tcagaaacga atgtccccgc caaatatcca tgttctgcca cgcgcggaat
     2281 tggctgggtg ggtctcgtca ccaagacctg gcatcatgtt gagcttattc agcgcctccg
     2341 aataatctct gttcatcagg ttttgtaccg aaagtttcag tgttaggttg cggttgtact
     2401 cgtaagtacc atagagatcg ataatcgttg ggatctgcgg caaatcctgt ttgattattg
     2461 cgccggtatg ttcgtcctgc tcaaaatcag gcgacaggcg acgagccttg cctgtgtatt
     2521 ttatgatagt gcccagggtc aacgcgttat cgaagaagcg aacaccagta tccagcgtca
     2581 tgtattttct gggcagttcg gtcatatccc ctgcgccaaa gtaggtgctg gcgatggagg
     2641 ttggctggtc tgtttgctgc tggctgaaag agagtcggcc aaaagcaaaa cctgcatcat
     2701 aatccatctc cagttcgaag ccctttgcga taacgtcgct tgccgagtta acgtagatgt
     2761 acatattgtc gctgtattca tcgctaatgc cctcccagtc attgccaatc acctcaggca
     2821 tactgcattt acgacctcca gaacaaacca gataagactc gctgtagatg tagttctgga
     2881 tcctgctgcg gtacgccaga gccttaaagc gcagggcatc ctgttcgacc agtaaatctc
     2941 tggtatcaat gttaaaaccc gcctgccagg tttctgcacg ttcaggcttc aggaatgggt
     3001 tcatggatgc gcctcctgaa ttagagaaga acatctcctg gatgttcggg gcgcgcatgg
     3061 atttgctgta gccaatgaac ggctgaagcc atggtgttac ctgagcagaa agctgaactg
     3121 aagggttgaa gccaccctct ttatcgtcaa tatcgtagct accctgtggc acgcagataa
     3181 cgcgtgaatc gcaggcgggc ttgtaccctg tgatcctgtt acgtgtgtag ttgagattga
     3241 aatctgcctc ccagatgccg cgcgtaacct tcagcccggt atacagcgct gaaatatcct
     3301 gctggccgga gggtgcgaac ggattgttct cgatcgattc ctgattcgct ttcgggtcgc
     3361 ccgccgctga gtgaatggtc cggtcatagc gggtacgcat cagtttgctg cccagcataa
     3421 actccaggtc attgtccgcg acagtgaacc ggctggtatt gttgatatcc agcgcgtcag
     3481 aacgattttg cgctgaggtt ttgtagaaag tatacagcga gccatcacga tacttttgat
     3541 taccgcgact ggtactggcc gttacgttaa agtcaattaa ttcagaaaaa ggggtgtaat
     3601 gatatttaat gtaatagtcg tcactggtta tatcacgacg tgtaaattta ttttcatagg
     3661 ttcgagcgga aagttcgaag ctgttaaatt cgtctggtct gatatccatt ttatacagtt
     3721 gggatttggg gttctgcttc atatatttat cataaccaaa ctctttgctg ttaattccag
     3781 aaccatttga gaaattagag tacacagaac tgccgctcac agcagccata acgccaatgc
     3841 ttcccgtatc cgtaaaagcg tcgctttttc caccaagggc gatcattccg ctgcgtccca
     3901 gcccattact accgacagag aaacgtgaac gaaggccata tgtattaccg ttaaatatta
     3961 catcgtcaac gccaatagta cgcatatttg cactaccggc aagggcgttg atcccttcag
     4021 agccactgct gtcaccgcgt gtaacatcaa ctgctaccag taagttagga tctataagta
     4081 cgccagccat attgttagtt gaaccatgcg tcgttgttcc ggaggtagag gttccgtaaa
     4141 aactctgggt aataccatcg accatagtgt ttacacgacc aaatccgctc atgcctcgaa
     4201 tattcacact gattgctccc tgaccaggat ctatttgagt ataagtacca ggcatactac
     4261 gcactgttgc gtcaagtgat tgcaggtttt tgtccgttgt ccgggaactg gtcgcgcctg
     4321 ttttctcgag agcttcctgc tcactggtcg tcttcccgac agaaacgctg agcggactga
     4381 attctgccgc tccttttttg tcggaattag ttttttcact gatggattgt gagtaagcac
     4441 cgcagctgag caataatatg cctgagccga tagccagttt tataacgttc ataaatttcc
     4501 ttgggtata
//
LOCUS       DAZPLI010000235         4483 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZPLI010000235 DAZPLI010000000
VERSION     DAZPLI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.238"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(8..1069)
                     /locus_tag="ACHUU3_004065"
     CDS             complement(8..1069)
                     /locus_tag="ACHUU3_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR2083993.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1066..2463)
                     /locus_tag="ACHUU3_004066"
     CDS             complement(1066..2463)
                     /locus_tag="ACHUU3_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR2083994.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2618..3616
                     /locus_tag="ACHUU3_004067"
     CDS             2618..3616
                     /locus_tag="ACHUU3_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR2083995.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3727..>4483)
                     /gene="ompG"
                     /locus_tag="ACHUU3_004068"
     CDS             complement(3727..>4483)
                     /gene="ompG"
                     /locus_tag="ACHUU3_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFR2083996.1"
                     /translation="AEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEV
                     HYQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGW
                     LSMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFS
                     TQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNG
                     LSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1092 a         1229 c         1099 g         1063 t
ORIGIN      
        1 tattgcatta tttttcgctg ggtgttttgc ctttttgcag tgtttctttc acctgaccga
       61 taagctgacg acggaaatcc ccgaggcgag gtttgtcatc gtcaatccac ggcagcgggc
      121 ggcaaagctc catagcttta atcccgagtc gtgccgtcag aagtcctgca ccaatcccct
      181 gagctgcgcg ggtagacaaa cgagcagcga gatcttgcga catccagtcc atccccactt
      241 cgcgcaccag ttcgctggct ccggcaaaag cgatattcag caataccagc ttaaacagac
      301 gcaaacggct gtaatacccc agttcaatgc catacagcgt ggcgatgcga ttaattaaac
      361 gcagattgcg ccaggcgata aacgccatat cgaccaacgc cagcgggctg accgcaatca
      421 tcaacgttga ttccgccgcc gaacggctga tttcgcgccg cgcctgggca tctaaaactg
      481 gctggaccaa atgcgcatac aggctgacca cttcacggtc gttttgcgtt tcatggattg
      541 aggcatacca gcgttgcagc gccggatgcg actgatcaat acccgcctgc tgcgccagtt
      601 tttcgcaaaa tgcgcggcct ttgcccgtgc cgtggctatg caataaatca cgcgcttcgt
      661 cgcgttcatg ggcgcgctgt cgcaagcacc ataagcactg ccactctgtt accacagaac
      721 ctacgccagc gccgataatc aatgccccag cggcacatcc acccagcgct acccagtcct
      781 gggtttgcca ggcattcatt gtccactgta caccctgccc gacaacactt gcgccaaaca
      841 gagccagccc gcccatcacc attttgcgcc acaggctgcg tttcggacgt aacgctgcat
      901 ccattaccgc ttcaacttgc ccctcttctt caggcgcttc atcgagcgtg gccggggcaa
      961 aattttgcgc ctgattttcg tcaaaggtct gctgcgccct gaattttgga ttctgatcga
     1021 cctccagcgg accgtcgaaa tcaatacgtg gttttaacgg ttcggtcatc gcaatttatc
     1081 tcctattaaa aattccagcg cggcatcaag acgaatatgc ggcagcggtt tgtcgacatc
     1141 catcacctgc gggcgaaacg cttcaaactg gaacccttgc ttgtcccaga acgcctgacc
     1201 gggcaaacgc gcaggaactt caccggggta aacggtgaga ggcgcaccat cgctgagccg
     1261 attaccgcgc aatgccggaa ttttctcgcc gttgacatca ataatgccgc tggtggtcgc
     1321 ctgcaccgag gccagcccca ggcaatccat actaatccct tcaaacgccg cattttgcca
     1381 ggcatcctga atcagttgtt gcagtaatga aaccatattg gcgtgctgat cgaccgttac
     1441 atggtccgct ttcgttgcgg caaacagcag tttgtcgata accggcgaga ataagcggcg
     1501 aaagagcgtg cgctgcccgt agtggaaact ttgcatcagc tgcgtcagtg ccagacgcat
     1561 atcattaaat gcctgtggcc cactgttgag aggttgcagg caatccacca gcacaatctg
     1621 gcggtcaaag cgcagaaaat gattcttata gaaccccttc accaccttct cgcagtaata
     1681 attaaaccgc tcgcgcagca ttccggcatt ggtgtgctta tcggcctgcg ccagtttgga
     1741 ctcgccccag gtatcgacat ccggccacgg gaagaattgc agcgcgggcg cacctgccat
     1801 atctcctggc aagacaaagc gccctggctg aataaagtgc agcccctgct gtttacagtg
     1861 gtggagataa tcggtccacg cggcggcaat gtctgccagc cggttttcgt cggcaggtgc
     1921 tagcgggtcc agcccttcgc acatcattcg ccatttgacc gaccattcgc cgcgctgacc
     1981 attgagtaag cccgtcatct ggcgtgacca gcttaaatag tcctgcgcca gcatcggcag
     2041 gtcgagcaac cattcgccgg gataatccac aatctccaga tacagcgtgg aggtgtcttt
     2101 gaagtggcgt agcagcgaat cgttcgattt aaaacgtagc gccaggcgga tttcactgac
     2161 gccgcgcgta ggcgtcggcc aggcgggagg atcgccatat aactgcgcca gtccttcatc
     2221 ataggtgaag cgcggaatgc caaagtcacg ctgaggaatg cgttttacgc ccagcaggcg
     2281 ctcttcacgc accgcgctta acagcggcaa gcgtgctccg gcgtgaatat tgagcaactg
     2341 attgaccatc gcagtgataa acgctgtttt gccgctgcgg cttagtccgg ttacggcgag
     2401 gcgcagatgt ctgtcgacac cccgattcac cagcgcatta agttcatttt taagtcgctt
     2461 cattgctgtc ctgtgctgct ctgaagacca gaaaaaaatc attcgtaagg atatgaatgt
     2521 gggggccagg ccattgatta tcaactgccc gtgttgtcat cgacgggcga actccataag
     2581 atgtgcagca ttcacgttcg gacggagttc ctgacctatg tcccctacta tttatgatat
     2641 tgccagggtt gcaggcgtat caaaatccac cgtatcacgc gtgctgaata agcaaactaa
     2701 tatctccccg gaagcgcgcg aaaaagtgtt acgggccatt gaagaattac agtatcagcc
     2761 aaacaagcta gcccgcgcgc tgacctcttc gggttttgat gccattatgg tgatttctac
     2821 ccgttcgacc aaaactacgg cgggtaatcc gtttttctct gaagttttgc atgccatcac
     2881 cgccaaagct gaagaagaag gtttcgacgt gatattgcag acgtcgcaca acccggcaga
     2941 agacttacaa aaatgtgaaa gcaaaattaa gcagaaaatg attaaaggca ttattatgct
     3001 aagttcgcca gcggatgagt cattttttgc ccaactcgat aaatacgata ttccggtggt
     3061 ggtgattggc aaagttgaag gtcaatatgc ccatgtttat tctgtcgata ccgataattt
     3121 tggcgacagc attgcgttga ccgatgcatt aattgaaagt gggcatcaaa atattgcctg
     3181 cctgcatgct ccgcttgatg tccatgtttc agtggatcgg gtaaatggtt ataagcagag
     3241 cctggctgcg cataatattg cagtgcgtga tgaatggatt gttgacggtg gttataccca
     3301 tgaaacagca ctgcaagccg cgagagaatt attaagccag tcaccgttgc ctgaggcggt
     3361 gtttgccact gacagtctga aattaatgag tatttatcgt gcggcagcag agaaaaatat
     3421 tgctattccg cagcagttag cggtggtggg ttatagtaat gaaacgctgt cgtttatatt
     3481 aacgcctgca ccgggcggca tcgatgttcc gacgcaggag ttaggtcaac aaagctgcga
     3541 gttattattc cgcttaattt ctggaaaacc gtcaccacag aatattaccg ttgccacgca
     3601 tatgacgctg aaataaatac cagctacctt gcctgaatgc cggatgcgat gctagcgcat
     3661 cttatccggc ctacatttat agatcggata aagtactcgc gccgcatccg gcaatttagc
     3721 ccattatcag aacgagtaat ttacgccgac acctgcataa tggaatttat cactgtctcc
     3781 ttcgtcgtga tcctgccact caaacgcgta ttccagcgaa acggaaagtc cgttctggaa
     3841 atcataaccg taaaataaac ctacacggtt aaaatcatgg ccttcacgtt caatatcatc
     3901 ctgccagtcc cagttactcc agcgatccag cccaatgcgc gtatacggcg tcaccgagtg
     3961 gttgccgagc gtcagcggca aataggcgcg aatttcttgc gtggaaaact caccgttatt
     4021 gcggctgtcg tccatattga agccgcgctc gagataatag ttcactcgca aggcaaccgt
     4081 ttcgttgaag gtatattgca gacctgtttc cgtttcgaca cgggtatcag cgtaaccggt
     4141 agtgttcaga tcgttggcaa atttatacat cgacaaccaa ccgttgaaac gtaaatcgtc
     4201 agtcagtttc acatcccagt ctggcgcgat tttccagcgc tgcatattcg ccgtgtcttt
     4261 acccggttca tcaacgtagt gataaccata attacggaaa ccgccggtca ggccgaaact
     4321 gaaatcatcg ttttcgagga actgataatg cacctccagc tccgggcgat caaaccacgt
     4381 tccacgttta cccgcgctat aatctaccgg cccttcctga taataggcca gagcaattct
     4441 ccacggcccg ttggcggcat taaaatagac tgaaggctcc gcc
//
LOCUS       DAZPLI010000236         4409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZPLI010000236 DAZPLI010000000
VERSION     DAZPLI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.239"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..105)
                     /locus_tag="ACHUU3_004069"
     CDS             complement(<1..105)
                     /locus_tag="ACHUU3_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2083997.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNV"
     gene            complement(173..2122)
                     /locus_tag="ACHUU3_004070"
     CDS             complement(173..2122)
                     /locus_tag="ACHUU3_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR2083998.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2207..2542)
                     /locus_tag="ACHUU3_004071"
     CDS             complement(2207..2542)
                     /locus_tag="ACHUU3_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR2083999.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2542..2898)
                     /locus_tag="ACHUU3_004072"
     CDS             complement(2542..2898)
                     /locus_tag="ACHUU3_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR2084000.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2898..4394)
                     /locus_tag="ACHUU3_004073"
     CDS             complement(2898..4394)
                     /locus_tag="ACHUU3_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR2084001.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKQGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1020 a         1313 c          970 g         1106 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt
      361 agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat
      421 agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac
      481 tgttcagtgc atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct
      541 gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg aggcccttta
      601 atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac
      661 gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta
      721 ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt
      781 tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa
      841 gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc
      901 gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg
      961 tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca
     1021 tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg
     1081 cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat
     1141 attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc
     1201 gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca
     1261 gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt
     1321 gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc
     1381 gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca
     1441 acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg
     1501 ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa
     1561 tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt
     1621 taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact
     1681 gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg
     1741 catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca
     1801 caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct
     1861 ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg
     1921 ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg
     1981 cggttccggc acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca
     2041 gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct
     2101 tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc
     2161 agttctgtaa actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac
     2221 caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac
     2281 gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac
     2341 gcgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac
     2401 tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct
     2461 cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta
     2521 tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc
     2581 tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac
     2641 atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt
     2701 gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt
     2761 ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat
     2821 cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt
     2881 tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc
     2941 accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc
     3001 gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca
     3061 ataccggcac gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga
     3121 gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg
     3181 tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt
     3241 tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg
     3301 gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa
     3361 agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc
     3421 agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca
     3481 aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc
     3541 gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag cgttccctgt
     3601 tttgcggtat agacatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca
     3661 ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag
     3721 tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg
     3781 gcagcaccga cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa
     3841 ccataataat tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc
     3901 agcgtcacca cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc
     3961 gcttccttaa tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt
     4021 acacgggagc gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc
     4081 gtcaccctga ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca
     4141 ccgaaaggat ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca
     4201 caaatctggc gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct
     4261 gcatcgttgc tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc
     4321 gcagaattgt tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta
     4381 aaacttatcg tcatgatttt ttagcctta
//
LOCUS       DAZPLI010000237         4352 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZPLI010000237 DAZPLI010000000
VERSION     DAZPLI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.240"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHUU3_004074"
     CDS             complement(<1..215)
                     /locus_tag="ACHUU3_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR2084002.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(318..393)
                     /locus_tag="ACHUU3_004075"
     tRNA            complement(318..393)
                     /locus_tag="ACHUU3_004075"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(358..360),aa:Thr,seq:ggt)
     gene            complement(400..474)
                     /locus_tag="ACHUU3_004076"
     tRNA            complement(400..474)
                     /locus_tag="ACHUU3_004076"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(439..441),aa:Gly,seq:tcc)
     gene            complement(591..675)
                     /locus_tag="ACHUU3_004077"
     tRNA            complement(591..675)
                     /locus_tag="ACHUU3_004077"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Tyr,seq:gta)
     gene            complement(684..759)
                     /locus_tag="ACHUU3_004078"
     tRNA            complement(684..759)
                     /locus_tag="ACHUU3_004078"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(724..726),aa:Thr,seq:tgt)
     gene            1121..2071
                     /gene="coaA"
                     /locus_tag="ACHUU3_004079"
     CDS             1121..2071
                     /gene="coaA"
                     /locus_tag="ACHUU3_004079"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR2084003.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2100..3065)
                     /gene="birA"
                     /locus_tag="ACHUU3_004080"
     CDS             complement(2100..3065)
                     /gene="birA"
                     /locus_tag="ACHUU3_004080"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR2084004.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3062..4090)
                     /gene="murB"
                     /locus_tag="ACHUU3_004081"
     CDS             complement(3062..4090)
                     /gene="murB"
                     /locus_tag="ACHUU3_004081"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR2084005.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4225..4340)
                     /gene="rrf"
                     /locus_tag="ACHUU3_004082"
     rRNA            complement(4225..4340)
                     /gene="rrf"
                     /locus_tag="ACHUU3_004082"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1106 a         1093 c          981 g         1172 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gcggtgatat
      241 caccacatca accaggcaac atgcccgact tgttgaatgc aataaacaga aggaaaaaac
      301 agggaggaga aaagaagtgg tgctgatagg cagattcgaa ctgccgacct cacccttacc
      361 aagggtgcgc tctaccaact gagctatatc agcacatctt ggagcgggca gcgggaatcg
      421 aacccgcatc atcagcttgg aaggctgagg taatagccat tatacgatgc ccgcatcctg
      481 gaactcggct acctgatttt cattctgcac tgaatatcga gagaagctct ctttattcga
      541 gccggtaagc gaacttatcg tctcgggcta cgccatcgcg tggccgaaat tggtggtggg
      601 ggaaggattc gaaccttcga agtctgtgac ggcagattta cagtctgctc cctttggccg
      661 ctcgggaacc ccaccggact tgatggtgcc gactaccgga atcgaactgg tgacctactg
      721 attacaagtc agttgctcta cctactgagc taagtcggca tcaagtagcg cgcattctat
      781 ggagacatgc gagttcatgc aactaaaaaa ttgcataatt tgttttattg gtcacatttt
      841 atgcgacacg atgaagaaac agccgaacgg agggcagatt agcacacttt ttcaacatca
      901 ttgtgctcaa caatgcgctc ctgctaaacc ataattcttt ttatcagatg gaatatctgt
      961 cacattgctt ttcaacgata gcttcctggc agagattttt tcttattatt cctccccatc
     1021 tggtgttacc ctcctgccca ttaacccatt caacagaact gtgacgcgcc atggcaaata
     1081 tcgctttgcc gatagagcta tgaccgccag aaacatgctt atgagtataa aagagcaaac
     1141 gttaatgacg ccttacctac agtttgaccg caaccagtgg gcagctctgc gtgattccgt
     1201 acctatgacg ttatcggaag atgagatcgc ccgtctcaaa ggtattaatg aagatctctc
     1261 gttagaagaa gttgccgaga tctatttacc tttgtcacgt ttgctgaact tctatataag
     1321 ctcgaatctg cgccgtcagg cagttctgga acagtttctt ggtaccaacg ggcaacgcat
     1381 tccttacatt atcagtattg ctggcagtgt cgcggtgggg aaaagtacaa ccgcccgtgt
     1441 attgcaggcg ctattaagcc gttggccgga acatcgtcgt gttgaactga tcactacaga
     1501 tggcttcctt caccctaatc aggttctgaa agaacgtggt ctgatgaaga agaaaggctt
     1561 cccggaatcg tatgatatgc atcgcctggt gaagtttgtt tccgatctca agtccggcgt
     1621 gccaaacgtt acagcccctg tttactcgca tcttatttat gatgtgatcc cggatggaga
     1681 taaaactgtt gttcagcctg atattttaat tcttgaaggg ttaaatgtct tacagagcgg
     1741 gatggattat ccacacgatc cacatcatgt atttgtttct gattttgtcg atttttcgat
     1801 atatgttgat gcaccggaag acttacttca gacgtggtat atcaaccgtt ttctgaaatt
     1861 ccgcgaaggg gcttttaccg acccggattc ctattttcat aactacgcga aattaactaa
     1921 agaagaagcg attaagactg ccatgacatt gtggaaagag atcaactggc tgaacttaaa
     1981 gcaaaatatt ctacctactc gtgagcgcgc cagtttaatc ctgacgaaaa gtgctaatca
     2041 tgcggtagaa gaggtcagac tacgcaaata atttgcaggg gagcgaatac tcccctttct
     2101 tatttttctg cactacgcag ggatatttca ccgcccatcc agggttttat tattccatcc
     2161 tgctcaagta ataaagcccc ctgtttgtct attccgcgtg aaatgccaaa tatttcttta
     2221 tcaccaatga taagtttcac tgggcgatta ataaaattat ccagcttttc ccagcgcgac
     2281 agataaggtg ccaatccttc ttgttcgaag agttccaacg cagcacgtaa ttcacgtatt
     2341 agcatggccg ccaacgtatt acgatcgaga ttgatccccg cttcctgcag cgtgatccac
     2401 ccctgattaa cgacactctc ttcaacacgg cgcattgcca tgttgatccc ggctccaatg
     2461 actatttgcg ccgcatcgcc agttttgcca gtcagctcca caagaatgcc tgccagcttg
     2521 cgatcctgca gatagaggtc attaggccat ttgacacgaa ctttatctgc tcccagcttg
     2581 cgtaatactt ccgccatcac gataccgata accagactta aaccaatcgc cgccgccggg
     2641 ccttgttcca gacgccagaa catcgacaaa tataagtttg cgccaaaagg cgaaaaccat
     2701 ttccgccccc ggcgaccacg gccagcctgc tggtattctg caacacaggc atcgcccgat
     2761 ttaagctctc cgatacgatc aagaaggtac tgattcgtgg agtcaataac tggcagcacg
     2821 gctacactac cgccatccag ctgacccaat atctgttcag cattaagtaa ctggatgggc
     2881 tcaggcaggc tgtatccttt acccggaacg gtaaagacat caacgcccca gtcacgcagt
     2941 gtctgaatgt gtttattaat agccgcccgg ctcattccca gcgtttcacc caactgctcg
     3001 ccagagtgaa attcaccgtt cgctaacagg gcaatcaatt tcagtggcac ggtgttatcc
     3061 ttcatgaaat tgtctccact gcgctcactt cacctgatgc accaataaag cggacttcag
     3121 gctcaagcca gacattaaat ttttcaccca ctttttgtct tacatggtgt gccagttgca
     3181 ccacatcttc gctttttgca ttgtcttcat taatgagaac taacgcctgt tgacggtgca
     3241 ccgcagcccc acccatttgc atccctttta gctggcactg atcaataagc caacctgctg
     3301 ccagttttac tgaaccaccc gcctgggggt aatttggtgc tgttggaaat tgcgccagta
     3361 atgctttagc cgtttcggca gatacaacag ggtttttgaa gaaactaccg gcattgccat
     3421 tcacttttgg atcagggagt ttggtggtgc gcatatgaca caccgcatta aatacttgct
     3481 gtggcgttac tgttgtagga tccagacgag ttaagtcacc atacgttagt acaggttgcc
     3541 actcttttgg cagacgcaga cctacggcta caatggcgaa gcgatcctgg tattcatgtt
     3601 taaaaatact gtcgcgatag ccaaaacggc actctttggc agttaagcgc acttgcttgc
     3661 ctgtcgccag ttcaacacaa tcaacataag cgcaaactcg ctgtaattct acgccataag
     3721 caccaatatt ctggataggt gatgagccga cacaaccagg aattaatgcc agattttcca
     3781 gaccaggcat accttcctgc aaagtgtatt ttaccaggcg atgccagttt tctccggctc
     3841 ctacatgtaa ataccacgca tcaggttcat catgaatttc gatacctttg atccggttga
     3901 tgatcaccgt gccacgatag tcttccagaa aaagtacatt acttccttca cccagaataa
     3961 gaacgggttg tccttctgcg gttgcatgct gccaggcatt gagtaattgt tgttcgtctt
     4021 cggcacatac aatgtgctga gcattatgat caatgccaaa tgtgttccag ggttttaagg
     4081 agtggttcat agctgctttc ctgatgcaaa aacaaggcta gtttaccgta tctgtggggg
     4141 gatggcttgt agatatgacg acaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     4201 ggatggcctt ctgcttaatt tgatgcctgg cagttcccta ctctcgcatg gggagacccc
     4261 acactaccat cggcgctacg gcgtttcact tctgagttcg gcatggggtc aggtgggacc
     4321 accgcgctac ggccgccagg caaattctgt tt
//
LOCUS       DAZPLI010000238         4328 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZPLI010000238 DAZPLI010000000
VERSION     DAZPLI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.241"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..581)
                     /locus_tag="ACHUU3_004083"
     CDS             complement(<1..581)
                     /locus_tag="ACHUU3_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR2084006.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPN"
     gene            complement(644..1093)
                     /locus_tag="ACHUU3_004084"
     CDS             complement(644..1093)
                     /locus_tag="ACHUU3_004084"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR2084007.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1090..4182)
                     /gene="ebgA"
                     /locus_tag="ACHUU3_004085"
                     /pseudo
     CDS             complement(1090..4182)
                     /gene="ebgA"
                     /locus_tag="ACHUU3_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1028 a         1090 c         1167 g         1043 t
ORIGIN      
        1 aagttcggga tcatcgcgtc aacggtgatg gcgtcagcag gctgtacgcc gccaaccagc
       61 gccgtacctg ccagtaaaat gtaggagagc gttaacagca gcatcagcgt tgaagtgacg
      121 gaggtaattg gccccaacat tttcgccccg ttggtggaaa cccaggtgga gaaggcgaac
      181 agcaccatac tgataatggt ggtggcaacc ggcgtcataa tatattcgta tccgaggaag
      241 gcatacgaag cataagcaat aacgcgcggc aacagtgagg tgaaaaagaa caggtttacg
      301 aaccagtagg tataggcagt aataaatgcc caacgtccgc ccagcgaact ttttacccat
      361 gcgtagacac cggcttctga gtttttattt aacgaaacaa attctgcgat gatcagacag
      421 aagggaataa aataaaaaat cgtcgcgagg aaaaacatcg gtgccgaggc cagtccaaga
      481 tcaatattat tattgataac gttgttaaag ctgtaaacgg cggcaaaagt cagcgagagc
      541 aagccgaatt tgccgattgt attacgtttg gtatcagaca taacacatct ccgaaaatga
      601 gtagggtagc ggtggcggca aactcctttt gccgccgtag ttgttatttg ttatggaaat
      661 aaccatcttc gatggtgact ttgagaacca cttttttgac cgcgttattg cagataaacc
      721 gatacgcttc atggatatcg caaataacga tttgcccttc gtggacctca acggtttctc
      781 cgcagccttt taaatattca cggtcagttt catcacgata atattccact acctgtaatg
      841 tctctttcgg cgcatattca attttttgct gcccttgcag gtagtaatgc acttcaaaat
      901 agcgtcgatg cccggtaaat agcgcatcgg tcgcggagtc tgtctccacg cggtaagtca
      961 atgagtcacc gatggagtgg gcgacgccag gctgaatgtt gtcgatattt tcaatcgctt
     1021 caacgcagcg ttgccacttc ttgccagagg cgtaaatctg gcggaactgt tctaagttat
     1081 cgatgatcct cattgcttat tctcgctgtg caaattcgtg gaaaagaacc ctgcgccgaa
     1141 ctcatacgac gccaggcttt gcgcggtagc ttctccgcca gaaaccggca gcaacgtaaa
     1201 gccgtagctg aagtcacgga accagacgcg ccaggagtcc agcacctcgc tgccccagga
     1261 gttggagccg aggccaagca gctggtgatc gaggttcagg gtgatgtcat cactgcgctg
     1321 cagctcgtta cagtgctggg cagcgtggat gttttcctag gtatagtgcc aggcgctgaa
     1381 gttaattggg cgctgcggaa ccaccagcag accgttaccg tggcggttag tcagtgccgt
     1441 ccagcggaca tgctgacggt taccgttgtt ctgcgggaag ggatagttct cgaacatggc
     1501 atcgacggtg ctgcgccaga tatcgatgat gttagcctgc tggctgtcgg cgtagttttc
     1561 gcccggtcca cgaccgtaat acgccacctg atcgtattcg ccgttaattc ccatggtgaa
     1621 accgatgcac ggaatgatgt gcggatagtc gccgtaacgc tcgccggaaa gcgccacgtt
     1681 aacctggcca tcggcagcga tgcgccagat gtaggtgcag cgcattccga aatcaaacac
     1741 cggcggggca ataaccgtgc ggctgatgat caacacttca ccctcgctct gttctacggc
     1801 aaagtcgcgc agatgttcct gcatgatctg caaatgattc ggctgccaca acccttcgta
     1861 ctcctgcttg tggttgtcga tcatcggctt gaagaagttg atctttggct cgcgggtcag
     1921 cagcgactcg ccatttacct gccaggatgt cggtttgcca ctcatttttg agaaggtgat
     1981 cgcgaagttg tagccgcgaa cggtgcagct caaacgatcg tcttccagcg tcagcggacg
     2041 cgcattattt ggtgcgaaag gcactggctg cgcggtgttt tccttcagcg ggaactgata
     2101 agtggcgata gaatgcccgg cttcgctgta gcgggtgcgg gaatctttgg tcaccgtaat
     2161 gttgaggaac gcttcgcggg cgtccagctg cggcagcgtg atctgcaagg gggcttcgct
     2221 gttcggcgca acgtcgcgca gtttaatctg ctgcgtcgcg agcgtttcac cttcggcgcg
     2281 cacctctgcg tgcagggtgt agtcatcaag cgtggtaaac cacagtttat tttcgacttt
     2341 caactcgccg cgagtcagat cccgcgcgtg gatttttacc ggcgcgataa cctgtttgta
     2401 ctctttcagg cccggtcccg gcgtctgatc ggaatagatc aaaccatcaa gacagaagtt
     2461 atagttgttg ggatagtcgc cgtagtcgcc gccgaattta taccagacat tgccgtggtc
     2521 gtcctgtgcc tggatcccgt ggtcgcacca ctcccagaca taatgaccct gaatgcaatc
     2581 gtgcttatag aagacgttct ggtactccgt cagcccgccc ggtccgttcc ccatcgcatg
     2641 agcatattca cagatgatgc gcggcttcgg atgcgggtat tcaccaaact cattcatcag
     2701 cggcacgcgg gtgtacatgg tggaaataat atcgaccact tcagcatcgc gatcttcttc
     2761 gtaatgcacc agtcgcgtgt catccagcgc cttcgctgcg tggtacatcg cgcggatgtt
     2821 acagccatag ccggattcat tgcccagcga ccagatgatg atcgacggat ggtttttctg
     2881 cgcgtgaata tggcgaacaa tgcgctcgac gtagactttt tcccactgcg gatcgtcggt
     2941 aatacggctg atatcgccga cattagcaaa gccgtgcgat tcgacgtcgg tttccgccat
     3001 cacaaacagg ccgtagatat cacacagttc gtaaaaacgc ggatcgttcg ggtagtgagc
     3061 ggtacgcacg gagttgatgt tgtgctgctt catcaactgg agatctttct cgacgcgatc
     3121 cattccaacg gcgcggcctt tgcgatgatc gttgtcgtga cggttgacgc cgtgcagcat
     3181 cacataacgg ttattgatcc agaacagacc gtcgcgcact ttgatatcac ggaagccaac
     3241 gcgttgtggt accacttcca gaacgttgcc gttggcgtct ttcagcgtca tgaccagatg
     3301 gtaaagataa ggggattctg ctgaccattg ctgcggctgt tcgacagtaa aagcaaagct
     3361 ggcgctggtc agtttttcaa ttgccaaatg atcaatggcg ctgctgtgca ccacgcgttc
     3421 gccatcaaac agggtatatt ccagcgtcgt gacgacaggg gaggcggcga gattttccag
     3481 caccacttcg caggaaagcg tggcatcgca ataggcttcg tcaaagtcgg tacgcacggt
     3541 gaaatcgtta atatgtgtta gttgttttcc gatcagataa acatcgcgga agatccccgc
     3601 tgaccaccac atatcctggt cttccacgta ggtagagtcc gcccattgca tcacgcgcac
     3661 acacaacagg ttgtcgccgg ttttaaccat cgcgctgatg tcaaactctg cggtcaggcg
     3721 actgcccttg ctgaaaccca catactgacc gttaacgtag acttcaaaat aggtttcgac
     3781 gccgtcaaat ttaatcagcg tctgtttacc ctgccagccg tcgctgaggg tgaaaatacg
     3841 ttgataggca ccggttgggt tatcgctggg aacaaacggc acatcgatgg ggaacggaaa
     3901 accttcgtcg gtatattgca gtttgccgtg accttccatt tgccacatgg cggggacggt
     3961 aatatgcccc cagtcagcca ttaactcaga ggtgaaggct tcaggcactt gcagcgggtg
     4021 gtcaaaaaag tggaaattcc actgaccgct taagggcaga aacaggctgc tggtttcgcg
     4081 ggcaaaggta cgcgcttgcg caacagaatc atatgaaaaa aagtacgcac gtggcgcaag
     4141 tcggttttcg tgggtgagct gaatgttttc ccagcgattc atagggcctc ccggtagaga
     4201 gaaatcggaa ccattcatgg tgatggtagt ttgtgtaatt gctggggagt ttagtaaaat
     4261 ttttactaaa aagtggcagg taaatgcatt tactttaact ggatcacgaa aataaacggc
     4321 agcactgt
//
LOCUS       DAZPLI010000239         4309 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZPLI010000239 DAZPLI010000000
VERSION     DAZPLI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.242"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..1870)
                     /gene="speC"
                     /locus_tag="ACHUU3_004086"
     CDS             complement(<1..1870)
                     /gene="speC"
                     /locus_tag="ACHUU3_004086"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR2084008.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDA"
     gene            complement(2033..2116)
                     /gene="yqgH"
                     /locus_tag="ACHUU3_004087"
     CDS             complement(2033..2116)
                     /gene="yqgH"
                     /locus_tag="ACHUU3_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR2084009.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2269..2976
                     /locus_tag="ACHUU3_004088"
     CDS             2269..2976
                     /locus_tag="ACHUU3_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR2084010.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3082..3157
                     /locus_tag="ACHUU3_004089"
     tRNA            3082..3157
                     /locus_tag="ACHUU3_004089"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3115..3117,aa:Phe,seq:gaa)
     gene            3355..>4309
                     /locus_tag="ACHUU3_004090"
     CDS             3355..>4309
                     /locus_tag="ACHUU3_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR2084011.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1114 a         1141 c         1037 g         1017 t
ORIGIN      
        1 gcgcatcctg ggggttcatc accactgacg ggaaactctg ctggcggaac atcgcttttt
       61 gtaggtcttt gacgtcgaaa ctgacataca gatcgtgcat ctcctgacac aactggcgca
      121 gggtgtagtc gcgatagcgc accggatatt tgttataaac gctcggcaac acctcagcca
      181 gcggcgagtc atcctcaata tgctgttcaa attgcgccag catcgccacc agttgtgcca
      241 gcttctcgtg gctttccgcc ggagttaata agaacagaat ggagttgaga tcgcacttct
      301 ccggcacaat gccgttctca cgcagatagt gcgccagaat cgtcgccgga acgccaaagt
      361 cgctatattc gccggtttcg gcattgatac ctggtgtagt gagtaacagc ttgcacggat
      421 caacaaaata ctgatccgcg gcatatcctt caaagccgtg ccacttcgcc cccggctcaa
      481 aactgaaaaa acggcggtcg ctggctaaca cagatgtcgg ataatcctgc cacaatttgc
      541 catcaacaac gggcgggata aacgggcgga acagcttaca gcgcgcaaga atagccttgc
      601 gcgactcaat acccaactca acgcactcag cccacagccg acgcccactc tccccttcat
      661 gaattttggc gttaacatcc agtgcagcaa acagcggata gaaagggctg gtagaagcat
      721 ggagcataaa ggcgttattc aaccgcttat gcgggcaaaa acgcgcctgt ccgcggatat
      781 ggttatcttt tttatggatc tgcgacgtct gtgagaatcc cgcctgctgt ttgtgcaccg
      841 actgagtcac aaagatcccc ggatcgtttt cgttaagttc taatagcagc ggcgagctat
      901 ccgccatcat cgggataaac tgttcgtaac cgacccacgc ggaatcaaac agaatgtaat
      961 cacacagatg cccaacggta tcgatcacct gacgggcgtt atagacagtt ccgtcatagg
     1021 ttcccagctg aataatcgcc aggcgaaacg ggcgcggcag ctcggctttt tctggcgcaa
     1081 cgtcgcgaat ttgctggcgc agatactctt cattaaaaca gtgcgcatca ataccgccaa
     1141 tgaaaccaaa cgggttgcgt gaagcttcca gatagaccga cgtcgccccc gcctgaatca
     1201 gcgcgccgtg atgattcgac ttatggttgt tacggtcgaa gagcaccaga tcgccacgcg
     1261 ttaacagcgc attcgtcacc actttattcg ctgccgatgt gccgttcagc acaaaatagg
     1321 ttttatcggc atgaaagact ttggctgcga atttctgcgc atccttcgcc gatccttcat
     1381 gaataagcag atcgcccaat tttacgtcag cgttacacat atcggcgcga aagacgttct
     1441 caccaaagaa atcgtaaaaa tggcgtccgg caggatgctt tttaaaaaac gcaccatgtt
     1501 gatgtccagg gcaagcaaag gtgctgttgc ccatctcaac gtactgcgtc agcgtgtcat
     1561 aaaacggtgg cagcaaattc tcttcatact gacaggctgc ggattccagc tccagccact
     1621 gctgctcgtt gccgttgatt accgccgtaa cgcccgcagg taattcaaca gcatgttcgg
     1681 aatacaaaaa caccggtaga tgaaaaccgg tgcgcttaag caacgcaaga atgccactgc
     1741 gactatccgc agcggtaatg acgactgccg cgacatccgt aaaatcagta tctcccaacg
     1801 ccaccacgcg gcgatgagta gaaagtcggg ataccagttc actactggcg gcaatattca
     1861 ttgatttcat aagcgcaaaa cccgtttcgg ggaagtaaga ataccggaca aagtgtaaaa
     1921 ccctgcccca tgagatatgg gtcaaactgg tcaccagctc cgaccgccag acatcagtaa
     1981 aagcagaaac gctctgattt tactgttgtc ctgcagtgaa cgtgcgttaa cttcaccgca
     2041 tgagcagtaa catagaaagg gaaacgtttc gcgcgaaacg gcgataagcg agagaatgta
     2101 aggagatggc gtgcatcggg caaactccgt acagagagga gaaaattcgc gcaatcatgg
     2161 cacctttcgc taaggcgtgc aagccaggac tttgcgacca gacaagccat caacaaggca
     2221 aaaagttcat aataagaaaa tcaaacaata cagctaacag gaactcttgt ggtcatcggc
     2281 ccttttatca acgctagtgc agtcttactg ggtggcgttc tcggcgcact gctcagccaa
     2341 cgcttaccgg aacgtatccg cgtctccatg acatctattt ttggtctggc atcgctgggg
     2401 attggtattt tactggttgt gaaatgcgcc aaccttccag cgatggtttt agctacgcta
     2461 cttggcgctc taattggcga aatttgtttg ctggagaaag gtgtcaatac agcagtcgcc
     2521 aaagcacaaa atctgtttcg tcactcacgt aagaagccag cgcatgaatc ttttattcag
     2581 aattatgtcg cgattattgt cctgttttgc gccagcggca ccgggatctt cggggcgatg
     2641 aacgaaggga tgaccggcga tccgagtatt ttaatcgcca agtcatttct tgatttcttt
     2701 acggcgatga tcttcgcctg ctcgctgggt attgcggtgt cggtaattag tatcccatta
     2761 ctgatcatcc agctaacgct agcgtgggct gccgcgctga tattaccgct gaccacaccg
     2821 tcgatgatgg cagacttcag cgccgtaggc ggtttattgc tgctggcaac cggattacgc
     2881 atctgtggca ttaaaatgtt cccggtggtc aacatgcttc cagcactcct tctggcaatg
     2941 ccgctttccg ccgcctggac cgcctggttt gcctgacaat gcgtgcaata gcggcaaagt
     3001 gatgatagat tgtgcagtct gcagtaaatt gaagaaattt gattgacgag acgaggcgaa
     3061 tcaggtttaa tgcgccccgt tgcccggata gctcagtcgg tagagcaggg gattgaaaat
     3121 ccccgtgtcc ttggttcgat tccgagtccg ggcaccaaat tcatatcaac ggacctccac
     3181 ggaggtccgt ttttcgtttc agaacgccac gatttaagcg ttctgcctac aaatcaattc
     3241 taccgaactc aaccagattc cccccacatc aaccccattg tgtgggtata attgcgggca
     3301 taccccagtt cgacagaatt tgtaccctct ttaacgcatt cagaaggctg aactatggca
     3361 ctgactgacg caaaaatccg ggctgcaaag cccactgaca aggcttataa actcactgac
     3421 ggggctggca tgttcctgct ggtacatcct aatggctccc gttactggcg tctccgttat
     3481 cgtattctgg gtaaggagaa gactctggca cttggtgtgt atccagaagt ttctctctcc
     3541 gaagctcgta caaaacggga tgaggcccga aaactgattt cggagggggt tgacccttgc
     3601 gaacagaaaa gagctaaaaa agtagtccct gatttacagc tctcttttga acatattgcg
     3661 cgacgctggc atgccagtaa taaacaatgg gcacaatcac acagcaataa agtactcaaa
     3721 agccttgaga ctcacgtttt cccctttatc ggcaaccggg atatcacaac actcaatacc
     3781 ccggatctgc ttatccctgt tcgtgctgca gaagctaaac aaatttatga aatcgccagt
     3841 cgtctgcagc aaagaatatc tgccgtaatg cgttatgccg tacagtctgg catcatcaga
     3901 tataatcctg ctctggatat ggctggcgca ttgactacgg taaaacgcca gcatcgcccc
     3961 gctcttgatc tttctcgcct gcctgaactt ttgtcgcgta ttggcagtta caaagggcaa
     4021 cctgtcaccc agcttgccgt tatgctgaat ttactggttt ttattcgttc cagtgaactc
     4081 agatacgccc gctggtctga aattgatatt gacaatgcca tgtggactat tccagccgaa
     4141 cgcgaacccc tgccaggcgt aaaattctca caccggggct ccaagatgcg aacaccacat
     4201 cttgtgccac tcagcaaaca ggctgtagcc atactgacag aactacagac atgggctggt
     4261 gaaaatggtc tgatctttac gggagcacat gacccgcgta aaccaatca
//
LOCUS       DAZPLI010000240         4288 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZPLI010000240 DAZPLI010000000
VERSION     DAZPLI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.243"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..317
                     /locus_tag="ACHUU3_004091"
     CDS             <1..317
                     /locus_tag="ACHUU3_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084012.1"
                     /translation="FTGVRAMAQGLQCWNAAGVLVVDLTDYNIRFMGTYTVKATSAQT
                     YTVSVPNMKTTGWFVHYAPASDTFNEWSAFCNNGSFTAVYLPTYAPLAGNYTFNVYKW
                     TA"
     gene            319..975
                     /locus_tag="ACHUU3_004092"
     CDS             319..975
                     /locus_tag="ACHUU3_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084013.1"
                     /translation="MAGFEVYNSAGALTIDSTNKSIMTGAVKAMGNLTDTGYYTGFTC
                     AFGNGGSLGFVMPSVVANRNTTQYWFQIQKDGAWCFPGAYMFQPGMGRFMTSSHTATP
                     TSGFLDVFSEEGTLIWSAASAATMPRIRGFLTAPAATDLSTAITVTSPVADPWFCWSQ
                     CPGNISDDGTVIGYSGLVIRRNSSTSFSLQYVSKNQKTYRQAMGNNGIQIALATFTGY
                     "
     gene            1032..1304
                     /locus_tag="ACHUU3_004093"
     CDS             1032..1304
                     /locus_tag="ACHUU3_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFR2084014.1"
                     /translation="MDIGAILALVISGCVFLYTIFRDNTKDTNDLLTRVATLETTIAV
                     LESSISRIEDDQDKMRDTLSKLETQIHELDVKIERIIAILQQKQRP"
     gene            complement(1343..1792)
                     /locus_tag="ACHUU3_004094"
     CDS             complement(1343..1792)
                     /locus_tag="ACHUU3_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFR2084015.1"
                     /translation="MSDLKTRLKEYEGTKEYQANLKYFRNGKFYPYADSLGCSTIGYG
                     HLIQAGEDFSTGITESEADKLLSRDLAKTIMQVQTLGLNLPDDWNDFIIIMTFQLGIG
                     GVKKFRKMLAALKAHDYLEAIKQAKCSLWYRQTPNRVNSMIAVLKNK"
     gene            1829..2263
                     /locus_tag="ACHUU3_004095"
     CDS             1829..2263
                     /locus_tag="ACHUU3_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012125115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084016.1"
                     /translation="MMSEWKYSDDWSEEEMLSGEYAGFVYIFQFEDGSNYVGAKQIYQ
                     RVKDARKLKPTSKENGWREYLSSSNVVKNKIENGEQYSKTILWCFATMREVMIVEAIL
                     ILHQMLKPECLNLAMMSKIRSPNAKDKKRLLGIVQELLEYLN"
     gene            2273..2773
                     /locus_tag="ACHUU3_004096"
     CDS             2273..2773
                     /locus_tag="ACHUU3_004096"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084017.1"
                     /translation="MASGINGIGSIRNYIQTQGVRLNNQFQNEIIKRSKLLSQRMQGD
                     LNNSVDRGAVGFTQRSILFFYRKVGRNSVTSTIMVKDIQAKYLYQVLVQPKAIDKFIP
                     TSSTRLTKQGNISGLKKNLLSERYKVVKGKNGKERLIDTAKKDTKKKTKRVIGLREKK
                     QRRLIY"
     gene            2849..3160
                     /locus_tag="ACHUU3_004097"
     CDS             2849..3160
                     /locus_tag="ACHUU3_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084018.1"
                     /translation="MNFEQHYGDLISKIKLNGMTPDGNSMPINKCFLDKKFQKVVKKS
                     NYQIDSYMNVIYEKKKDFKVGEVLEWELQGEKMPVFLIESKKLFVKGKHFWVYAVGII
                     E"
     gene            3157..3450
                     /locus_tag="ACHUU3_004098"
     CDS             3157..3450
                     /locus_tag="ACHUU3_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084019.1"
                     /translation="MISILIEFLKSGLDFFIKKKTIEQEVKKTNVEGQIETNKEEIEK
                     VTFHWRNALGFVLTLIILYNWIIVPVLDAFGIIVIQVPLGQLLQVLLIMVGGS"
     gene            3511..>4288
                     /locus_tag="ACHUU3_004099"
     CDS             3511..>4288
                     /locus_tag="ACHUU3_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR2084020.1"
                     /translation="MSDAITTAAIKKLYGLSAGQCNICRTSLFKEQVHIGQMAHVIAK
                     SPKGPRGDDLLANDNSYENLILLCANDHILVDRDPFTYTVEKLHQIKSEHEAYIQGAT
                     DNTLFSDKKRQSDVDFLNAYFHFTPFARVRSLVETLPNSFHINLLIFGDQFDSILRDF
                     PASYPLNDKLLHGHFQSFIDSYKNIDFIISGHLPISDNRNIQVFGGANHNLLCHFNYD
                     ELPSDKALIIEQDLISHKISLLNAYEGLINYLRAYYPEVEI"
BASE COUNT         1384 a          716 c          889 g         1299 t
ORIGIN      
        1 gatttacagg agtaagagct atggcacagg gattacaatg ttggaatgca gcgggtgtat
       61 tagtagtaga tctgactgat tataacatac gttttatggg tacttatact gtcaaggcta
      121 cttctgcaca gacctatact gtatcagtac caaatatgaa aactacaggc tggtttgtac
      181 actatgcccc cgccagtgat acctttaatg agtggtcagc cttttgtaat aatgggtcat
      241 ttactgcggt ttatttgcca acatatgcac cgttagctgg taactacact tttaacgtat
      301 ataagtggac ggcgtaagat ggcaggtttc gaggtatata attcagcggg tgcactgact
      361 atagatagta ctaacaagtc gataatgacg ggtgcagtaa aggccatggg aaatctaact
      421 gatacgggat actacacagg ctttacctgt gccttcggta atggtggatc gctcggtttc
      481 gttatgccat cagtagtagc taacaggaac acaacacagt actggttcca gatacagaaa
      541 gacggggcat ggtgtttccc tggtgcctat atgttccagc ctggcatggg caggttcatg
      601 acaagtagtc acacggctac ccccacatct ggttttcttg atgtgttcag tgaagaaggt
      661 actttaatct ggtctgctgc atcagcggca acaatgccac gtatcagggg atttttgaca
      721 gcacctgcgg ctaccgatct cagtactgcc attacggtaa cgtctccagt agctgatccc
      781 tggttctgct ggtcacaatg tccggggaac atatcagatg atggtacagt aattgggtac
      841 tctggtctgg ttatcaggcg taattccagt acttcattca gtctacagta cgtttcaaag
      901 aaccagaaaa cctaccgcca ggcaatgggg aataatggta tccagatagc attggcgaca
      961 tttacagggt attaatacaa aaacaataac aataacaata acaataacaa taacaacaaa
     1021 gggatttcag tatggatatc ggtgctattt tagctcttgt tatttctgga tgcgtattcc
     1081 tttatactat ctttagagat aatacaaagg atacgaatga tctattaact cgcgttgcta
     1141 ctcttgaaac taccattgct gtactggaaa gtagtatttc ccgtattgag gatgatcagg
     1201 ataagatgag agatacacta agtaaactgg aaacacaaat ccatgaactt gatgttaaga
     1261 ttgaaaggat tattgcaatt cttcagcaga aacaacgacc ataaaaaaag gcatagtatg
     1321 tcattcgtac tatgcctttt ctttatttgt ttttaagtac tgcaatcatg gaattcacac
     1381 ggtttggtgt ctgcctgtac catagagaac acttagcctg tttaattgct tcaaggtaat
     1441 catgtgcttt cagggctgct aacatcttac ggaatttctt caccccgcca atacctaact
     1501 ggaaggtcat gataataata aaatcattcc agtcatcggg aagatttaac cctaatgtct
     1561 gtacctgcat aattgttttg gctaaatcgc gagatagtaa cttgtctgct tcgctttcag
     1621 taatgccagt actaaaatct tctcccgcct gaattaagtg accatatcca atagtactac
     1681 atcctaaact atcagcatat ggatagaatt tcccatttct gaagtacttc aaatttgcct
     1741 gatactcttt agtaccttca tattctttta atcgtgtttt taaatcgctc atttactaaa
     1801 tacctataat gaatttatag gaatatttat gatgagtgaa tggaaatact cggatgattg
     1861 gagcgaggag gaaatgttat ccggtgaata tgctggtttc gtttatatct ttcagtttga
     1921 ggatggttct aattatgtcg gtgctaagca aatctatcag cgggttaagg atgcgagaaa
     1981 gctaaagccc acttcaaagg aaaatggctg gcgtgagtac ctcagtagtt ccaatgtcgt
     2041 taaaaacaaa attgaaaatg gtgaacagta ctcaaaaacg attctatggt gcttcgcaac
     2101 tatgcgagag gtaatgattg tagaggcaat actgatcctt catcagatgc ttaaaccgga
     2161 atgtttgaat ctggcaatga tgagtaagat tagatcacca aatgcaaagg ataaaaaaag
     2221 actactcgga atagtacagg aactattgga gtacttgaat tgaggtaaat atatggcaag
     2281 tggaataaat gggattggta gtataaggaa ctatatacaa acgcagggag ttagattaaa
     2341 taatcaattc cagaatgaaa taattaaacg ttccaagcta ctatcccaaa gaatgcaggg
     2401 tgatttgaat aatagcgttg accgtggggc agtgggcttt actcaacgtt ccatcctttt
     2461 cttctataga aaggttggga gaaattcagt tacatcgacc atcatggtta aagatattca
     2521 ggctaagtac ctttatcaag tactggtaca gccaaaggca attgataagt ttatacctac
     2581 ttcatctaca agattaacta agcagggcaa catttcagga ctgaaaaaga atcttttaag
     2641 tgaacgatat aaggtagtga agggtaagaa tggtaaagaa cgattaattg ataccgcaaa
     2701 gaaagatacc aagaagaaaa ccaaacgtgt aattggctta cgtgagaaga aacaacgtcg
     2761 ccttatctat tgacttctat aaggaagcca atgatggtgt aagggtaatt atgagcggta
     2821 ttcagggaac attcaggata agtagacaat gaattttgag caacattatg gtgatttaat
     2881 aagcaagatt aagctaaatg gcatgacacc agatggtaat agtatgccaa ttaacaaatg
     2941 ttttcttgat aagaaatttc aaaaggtcgt taagaaaagt aattatcaga ttgatagcta
     3001 tatgaacgtt atttatgaga agaagaaaga tttcaaagtt ggtgaagtac ttgaatggga
     3061 attacagggt gaaaagatgc ctgtatttct aattgagagt aaaaagctct ttgtgaaggg
     3121 taaacatttt tgggtatatg ccgtggggat tattgaatga ttagtatatt gattgagttt
     3181 ctgaagtcag gattggattt tttcattaag aagaaaacta ttgaacagga agtaaagaaa
     3241 acgaatgtag aagggcagat tgaaacgaat aaagaagaga ttgaaaaggt aacattccac
     3301 tggcgtaatg ccctcggttt tgtactaacc cttatcattc tctataactg gatcatagta
     3361 ccagtacttg atgcttttgg aattattgtg atccaagttc cattaggaca acttttgcaa
     3421 gtgctattga ttatggttgg tggaagctga aagatttttc ctttaatatg tgttttgagt
     3481 taataaaata gcaatctaag gatttgattt atgagtgatg caattactac agcagcaata
     3541 aaaaaattgt atggactatc cgctgggcaa tgtaatattt gccgaacttc tctttttaaa
     3601 gagcaggttc acatcggaca aatggctcat gtaatagcca aaagcccaaa aggaccaagg
     3661 ggcgatgacc tgttagcaaa tgacaattct tatgaaaatc ttatcctgct ttgtgcaaat
     3721 gatcatattc ttgttgatag agatccattc acttatacag tagaaaaatt gcatcaaatc
     3781 aaaagtgaac atgaggctta tatacagggt gcaaccgata atacattatt ttcagataaa
     3841 aaaagacagt cagatgtgga ctttttaaac gcttacttcc actttacacc gtttgcgagg
     3901 gtacgttcat tagttgaaac tttacctaat tcttttcaca tcaatcttct tatttttgga
     3961 gatcagtttg atagtatttt aagggatttt ccagcgagct atcctttaaa tgataaatta
     4021 ctccacggtc atttccaaag ttttattgat tcgtataaaa atattgattt tatcatatct
     4081 ggacatttgc cgatttccga taatagaaac attcaggtgt ttggtggtgc gaatcataat
     4141 cttctttgtc atttcaatta tgatgagcta ccatccgata aagcactgat aatagaacaa
     4201 gatttgattt cacataaaat atctcttctt aatgcttatg aaggtcttat aaattacctt
     4261 agagcttatt accctgaggt ggaaattt
//
LOCUS       DAZPLI010000241         4287 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZPLI010000241 DAZPLI010000000
VERSION     DAZPLI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.244"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            165..1847
                     /locus_tag="ACHUU3_004100"
     CDS             165..1847
                     /locus_tag="ACHUU3_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR2084021.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1899..3056
                     /locus_tag="ACHUU3_004101"
     CDS             1899..3056
                     /locus_tag="ACHUU3_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR2084022.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3022..3144)
                     /locus_tag="ACHUU3_004102"
     CDS             complement(3022..3144)
                     /locus_tag="ACHUU3_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084023.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESIINYSPFVLQHP"
     gene            3353..>4287
                     /locus_tag="ACHUU3_004103"
     CDS             3353..>4287
                     /locus_tag="ACHUU3_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
                     /protein_id="HFR2084024.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGL"
BASE COUNT         1292 a          908 c          846 g         1241 t
ORIGIN      
        1 aattacttaa tagaacagaa agtaatgtca acgctttatg gactgttatt tccctttttt
       61 tagctaaatc tgctatctct ttatgtgact aacttcactt acatccactt atttctcttc
      121 gtaaaattac ttcggaatta agtacaataa gaagaggaac atttatgaag tctgcattaa
      181 agaaaagtgt cgtaagtacc tcgatatctt tgatactggc atctggtatg gctgcatttg
      241 ctgctcatgc ggcagatgat gtaaagctga aagcaaccaa aacaaacgtt gctttctcag
      301 attttacgcc gactgaatac agtaccaaag gaaagccaaa tattatcgta ctaaccatgg
      361 atgatcttgg ttatggacaa cttccttttg ataagggatc ttttgaccca aaaacaatgg
      421 aaaatcgtga agttgtcgat acctacaaaa tagggataga taaagccatt gaagctgcac
      481 aaaaatcaac gccgacgctc ctttcattaa tggatgaagg cgtacgtttt actaacggct
      541 atgtggcaca cggtgtttcc ggcccctccc gtgccgcaat aatgaccggt cgagctcccg
      601 cccgctttgg tgtctattcc aataccgatg ctcaggatgg tattccgcta acagaaactt
      661 tcttgcctga attattccag aatcatggtt attacactgc agcagtaggt aaatggcact
      721 tgtcaaaaat cagtaatgtg ccggtaccgg aagataaaca aacgcgtgac tatcatgaca
      781 acttcaccac attttctgcg gaagaatggc aacctcaaaa ccgtggcttt gattacttta
      841 tgggattcca cgctgcagga acggcatatt acaactcccc ttcactgttc aaaaatcgtg
      901 aacgtgtccc cgcaaaaggt tatatcagcg atcagttaac cgatgaggca attggcgttg
      961 ttgatcgtgc caaaacactt gaccagcctt ttatgcttta cctggcttat aatgctccgc
     1021 acctaccaaa tgataatcct gcaccggatc aatatcagaa gcaatttaat accggtagtc
     1081 aaacagcaga taactactac gcttccgttt attctgttga tcagggtgta aaacgcattc
     1141 tcgaacaact gaagaaaaac ggacagtatg acaatacaat tattctcttt acctccgata
     1201 atggtgcggt tatcgatggt cctctgccgc tgaacggggc gcaaaaaggc tataagagtc
     1261 agacctatcc tggcggtact cacaccccaa tgtttatgtg gtggaaagga aaacttcaac
     1321 ccggtaatta tgacaagctg atttccgcaa tggatttcta cccaacagct cttgatgcag
     1381 ccgatatcag cattccaaaa gaccttaagc tggatggtgt ttccttgctg ccctggttgc
     1441 aagataagaa acaaggcgcg ccacataaaa atctgacctg gataacctct tattctcact
     1501 ggtttgatga ggaaaatatt ccattctggg ataattacca caaatttgtt cgccatcagt
     1561 cagacgatta cccgcataac cccaacactg aggacttaag ccaattctct tatacggtga
     1621 gaaataacga ttattcgctt gtctatacag tagaaaacaa tcagttaggt ctctacaaac
     1681 tgacggatct acagcaaaaa gataaccttg ccgccgccaa tccgcaggtc gttaaagaga
     1741 tgcaaggcgt ggtaagagag tttatcgaca gcagccagcc accgcttagc gaggtaaatc
     1801 aggagaagtt taacaatatc aagaaagcac taagcgaagc gaaataacta aaccttcatg
     1861 cggcggattt ttccgccgcc ttattgagcg agatagcgat gcacgttaca gccaagccct
     1921 ccagttttca atgtaatctc aaatgtgatt actgttttta ccttgaaaaa gagtcgcagt
     1981 ttactcatga aaaatggatg gatgacagca ctctgaaaga gttcatcaaa caatatatcg
     2041 aagcgtctgg caatcaggtc tattttacct ggcaaggcgg tgaacccact ctggctggcc
     2101 tggatttttt ccgtaaagtt attcactatc aacaacgcta tgcaggccaa aaacgtattt
     2161 ttaatgcatt acaaacgaat ggcattttat tgaataatga atggtgtgcc tttctcaaag
     2221 aacatgaatt tctggttggt atctcgatcg atggccccca ggagttacat gaccgttaca
     2281 gacgcagtaa ttcaggtaac ggtacttttg caaaagtgat agcagccatc gagcgtctga
     2341 aatcatatca agtagagttt aatacgttaa ccgtcattaa taacgttaat gtccattacc
     2401 ctcttgaggt ttatcatttt ttaaaatcta tcggcagtaa acatatgcaa tttatcgaat
     2461 tgctagaaac cgggacgccg aatattgatt tcagtggtca tagtgagaac acattccgta
     2521 tcattgattt ttctgtgcct cccacggctt atggcaagtt tatgtcaacc atttttatgc
     2581 aatgggttaa aaacgatgtg ggtgaaattt tcattcgtca gtttgaaagc tttgtcagcc
     2641 gttttttggg gaatgggcat acaagttgta ttttccagga gtcctgcaag gataatctgg
     2701 ttgttgaaag taatggagac atttacgaat gcgaccattt tgtctatcca cagtacaaaa
     2761 ttggaaacat taataaatct gaactcaaaa cgatgaacag tgtacaactg acagcgcaaa
     2821 aaaaacggat ttcagcgaaa tgtcagcaat gtgcatataa acctatctgc aatggcggtt
     2881 gtcctaagca tcgtattact aaagtaaaca atgagactgt ttcttatttt tgcgaaggtt
     2941 ataaaatcct tttttcaacc atggtacctt atatgaacgc catggttgag ttagctaaga
     3001 acagagtacc gctttaccac attatggatg ttgcaaaaca aatggagaat aattaataat
     3061 gctctctttt aactccattc ttagcctcct tcttatgacc ttaacaaggc acccgacata
     3121 aattcctgtc gggtgtttcg tcatcatcat cttcccttgc gaaaatcctg gcttacgtgg
     3181 tatgcgtcag ggcattagca atacccttga ttatcaatag cataaccata accataacca
     3241 ctgaaaatta aaaatttcta tatatttcat attgttaaat gatacttaaa taaatttcat
     3301 cagtaaatca ttgccataga taatcattgg atggcacaat caattttcgc taatgataac
     3361 cattcccatt acgttttata tgttgatagc aaagtatctc tgtctgctga agcccttttg
     3421 gctgcgtaag aataataaaa cttcagtgtt gttaatcatt attatcctcg caatgatcct
     3481 tggcgtggtt aaaattcagg tctggttaaa cgactggaat aatgattttt tcaatgcgct
     3541 aagccagaaa gaaaccgata agctttggca gcttgttctt tggttccctg ccctgctggg
     3601 gatctttgta ttaatatccg taaacaaaac atggttaatt aaactactga ctattcgctg
     3661 gcgcgaatgg ttaacagatt actaccttaa tcgctggttt gcagataaaa actactactt
     3721 cacgcagatc tacggcgagc ataaaaatac agataaccct gaccaacgta tcgctgaaga
     3781 cattctctta ctgatcagca aaacgctcag tctttcattt ggcttcatcc agtcacttag
     3841 catgctgatc acctttaccg ttattctttg gcaaagcgcg ggtacgctct cattcactgt
     3901 tggtggaaca gaatggaata tccaggggta tatggtctat accgtcgtgc tcattgtgat
     3961 cggtggaact ttatttaccc ataaggtggg taaacgtatt cgtccgctta atgtggaaaa
     4021 acaacgtagc gaagcgacat ttcgaactaa tcttgtgcag cataataagc agacagaact
     4081 gattgcgcta agcaatgctg agtctctaca acgccaggag ttgcgcgaca attttcatac
     4141 cattaaagag aactggcatc gtttaatgaa tcgtcagcgg tggcttgatt actggcaaaa
     4201 tatttattcg cgctcgctta gcgttcttcc ctactttctg ttattgccgc agtttattag
     4261 cgggcagatc aatctgggcg gactgat
//
LOCUS       DAZPLI010000242         4256 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZPLI010000242 DAZPLI010000000
VERSION     DAZPLI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.245"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..3511)
                     /locus_tag="ACHUU3_004104"
     CDS             complement(<1..3511)
                     /locus_tag="ACHUU3_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR2084025.1"
                     /translation="MDENALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQ"
     gene            complement(3963..>4256)
                     /locus_tag="ACHUU3_004105"
     CDS             complement(3963..>4256)
                     /locus_tag="ACHUU3_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR2084026.1"
                     /translation="NKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQMS
                     HQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1096 a         1094 c          877 g         1189 t
ORIGIN      
        1 cctgacgcct ctcaagacta ttataaatag cctcaatctc acccttcagg ttatcaccgc
       61 aataaaccat tgatgtatcg tatcgttcat atcgcgcttc ccgtgtcgca ctaaaccagt
      121 tgaacgatcc cacacacagc aaaccatcat caccaataac aattttgcta tgaacacggt
      181 ttaccagctt tgtagcaata cccagcgcat tcagtttctc cagcgccgct ttaaagttct
      241 gctgcttctc ttttcgcttc tcaaaatcat tatgttcagt gttgtagctt ctgtcagtga
      301 ctatcgtgac gttaattcca cgtgaacacg ccgcaatcat ggaatcaaga aaaccggttt
      361 gctccagcct ttgccaggtc agccatggag aaattatcgt gatgtgttta ctggtatttt
      421 caaatgtctg attcaggaaa ttatcatgtt gctccacacc atgaagtgtg tagattttgg
      481 tcccggcggt ttttaaatcc ttacgctctt tataatcaaa agagagcgca ttcttctctg
      541 actcaaagag gtattttgcc agtaatcccc gtggcgatga ggctggctgg acctcaaaca
      601 ggtccatatc gccgaagacc agaaaactgt ccttcgcacg ggagactgca acattcagca
      661 tgctgttatc gctatcaata aacccgccgt cttcatgttt tgaatagact ggcgagaata
      721 tcacaatcgc tctttccgct ccctgaagag agtgcacggt gcccactgtg agcgactttt
      781 cattcgtgcc tgtactgata ccttgtttgc ccagcacctg tttgatagtg gatacctgag
      841 cgctaaaagg cgtcacaata ccgacaactt catgaagcga tttaccgtaa tgcgcttcaa
      901 tattttgctg gttctctgcc aaccagaccg ctatcgtttc agcctcaagc aaattatatc
      961 gacttccact acttgccagc tctcctttac catcaatatg gagatacccc attgcgggca
     1021 ttaaattgct ctcttcacgc cctcttttag gcaacaactt accgtgatag cagagcgtat
     1081 tacagtatcc aataatattg tcgtagcacc ggcggtgttc atataggtac ataccacgag
     1141 ccagttcggg atcatattga tagcgcgaag caaactgcgc tattttcata acgctgccag
     1201 atgcggcact tttaccaagg tctgcgattg ccgtatattt ctcggtaatc tcttcttgcg
     1261 tactgccaga cagaattttt tccgccagca tgttaccgac atcaatcgca ggagcaatac
     1321 tccatattgg cgggatctgc tccgtatcgc caatcactaa tgccttctta gctaatgcaa
     1381 acgaggcagc agccacttca ggaagcacct gcccggcttc atcgacaatg agtaaatcgg
     1441 caaaatcata caaataactt ttctcgaatt tacgttgtcc tttgtgctca cttatctgca
     1501 tattaccggg cagcatatag catgtcatta ccacacatgg cgtgagtttc attcgacgtt
     1561 gccaacgggc ggttacccct ttagcacctt ttttcttctt ctcgtcctgc agatcatcaa
     1621 tgctggccat atccatcagc caacgacctt cccagtagtg agtcgtcagt aaaaatgcag
     1681 ggaagcgaat ctgcgtatca gccagttcat cggcctgtga gaagctcagt tcctcgtcgc
     1741 cctcataccc taaatcaaat gccagcctct gccactcctg aaccgcctgc tgttctttaa
     1801 gaacgatttc atgggcggag tcaatctgct gccggtatgt tgtttgctcg cgctcagcgg
     1861 aattgagcag cccatcaata ttacgttcga tagtttcagg atcagaccac tgatttcctg
     1921 caatcagcgc gcctaattta tcttcgagaa acagctgtat ttggtactgt cgcttattgc
     1981 gaaccgccgg gagccaggaa aataatgaat agatcagtga ttcaccggcg cgatattttt
     2041 tccattccgt tttagcactc ttcagtagag tgactttttg ttcttgtccg gaaagtaatt
     2101 tatttaaatt atcgagatat tgctcaatat cattagcaat aagctcacgc gcagcccgaa
     2161 tctggcttaa cgtttgccat gttgcgttca gtcttatcag ttgctcagat tttgctgcca
     2221 actgaccatg caggagttca atgacctttt caggggatga acactctttt ccaggaaagg
     2281 ctgccttagc cttttccaga taaaacagca gtgcatcctc tacatactct tttgactcaa
     2341 cctggttgaa gaaatcttca gtttgatatt ttttggctgc ctcagcttta cgactgcttg
     2401 agggaaaata agcaccgaag cttttcagct ctggcaacca tcgcccggcc atcgcacctg
     2461 aaccttgcga aaagtctttc ccgaatgcct caataatgtt cgttacagcc tggttattcg
     2521 ttgaagtcgc gataataacc ggaggctcag atttttcgag agccgctctg gcccactgcg
     2581 tggcgatgat agaaagcacc agcgtggttt ttccggttcc cggagggcca ttaacagcaa
     2641 ggatatcgcc atgtcttgca tccagaaaat ggcttaaggc atcgcgctga gcctttgcca
     2701 gcggaaactt atctccggag tgtccaagcc tgtcgctgaa ttttgctcct ggggccagca
     2761 aagactctgc agcatgaacc tctcgcgagg cgaagcgatt gaagagcggc acatccttat
     2821 tgcaaacaat caggtgatca taaagagaaa ggatatggga actggcaccg ccagattgag
     2881 ccgttttaac aatataaccg tgctcagcga gttcatattg ctcaggtttt tcaatccagt
     2941 cgccggcaac gctcttcagt agcctctctg agtcatacag atattgacgc cactcctgct
     3001 gcaaggcggc atatcgtgct tcccgttctt catccgtttc ggcagtctta tcaacgctgt
     3061 catcaaagtt gatagagaac gtggtatggg tcgtcttgta tttgtcatac tgcccaatct
     3121 caccaatcga aaatgctcct tttggcaaag gttcaagcag gtctctggga atggaggtcg
     3181 ctggcgtcgg atataaaaaa ccctcacggc ttagcaaggc tggcgtcact atcggggtaa
     3241 caatatcagg cgcacctgca gaacggtcct taccatgctg cagtaaccgg aaataaactt
     3301 ttgggcgcaa gatgacatcg accgtttcga catcgtcttt ttctccctta aaaaatttac
     3361 tgacaatcgc ttcgtcaaga cgtcccgccg ctatcccatg ccagtgagtg aaatttttgg
     3421 cgtctttccg ttcaaaactg ccctttcctg actcagcatc cgcaagcgag ttgcgccagt
     3481 atgaggtaaa ccctaaagca ttttcatcca ttaccagtcc tgtgactcaa tgaaatccat
     3541 tgatttatcg aatataaaac caccatatgg acaactattg tccatattga aaaagttgga
     3601 tgcaatgttt tgtttataat tcggtatcaa atgtactggt tgtacaaggt tcagaacaca
     3661 agcacattca acagtacgtc aggactattg atttcataac agtgaataca ttttttaccg
     3721 tttataatga ctgcctgtgt tggacaagga taccagaaca gccgaaaaca ccgggggtta
     3781 ttacctgctc tcatggaaac cagatattcc tggtgctaat cacagtcata aatagcctga
     3841 agaacatgac gaagggactt taatggaagc cggagtaact tgccagaaca tcaatcttcg
     3901 tcatcattta caaagaaaaa taatcagtta aagcagtccg ggcaatcagc ccggactgct
     3961 tactatttta atggattgtt aatctttgca tattcaaacg ggctgatacc cttatctctg
     4021 ttcgcatcca gaaaatctgc ccaccactgt agcatcagtc gacgttcttc cagatgttct
     4081 gctttatgga tatacgcagc acgtacacca ttacgctcct gatggctcat ctgacgttcc
     4141 acagcatcgc gggaccacaa acctgattca atcaatgcac tgcacgccat cgcccggaat
     4201 ccatggccac agacatcctg ggttgtgtca taccccatca ccctcagggc tttatt
//
LOCUS       DAZPLI010000243         4150 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZPLI010000243 DAZPLI010000000
VERSION     DAZPLI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.246"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..196
                     /locus_tag="ACHUU3_004106"
     CDS             <1..196
                     /locus_tag="ACHUU3_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084027.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
     gene            207..374
                     /locus_tag="ACHUU3_004107"
     CDS             207..374
                     /locus_tag="ACHUU3_004107"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084028.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            371..715
                     /locus_tag="ACHUU3_004108"
     CDS             371..715
                     /locus_tag="ACHUU3_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084029.1"
                     /translation="MTTFTREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            1007..1162
                     /gene="hokD"
                     /locus_tag="ACHUU3_004109"
     CDS             1007..1162
                     /gene="hokD"
                     /locus_tag="ACHUU3_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR2084030.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1383..1671
                     /locus_tag="ACHUU3_004110"
                     /pseudo
     CDS             1383..1671
                     /locus_tag="ACHUU3_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1710..1988
                     /locus_tag="ACHUU3_004111"
     CDS             1710..1988
                     /locus_tag="ACHUU3_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084031.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            1990..3048
                     /locus_tag="ACHUU3_004112"
     CDS             1990..3048
                     /locus_tag="ACHUU3_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR2084032.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSVYSESDIIPGEQTATSILKQR
                     TKNIALLPHVHQQQNPPQEKTVVSIAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            3049..3429
                     /locus_tag="ACHUU3_004113"
     CDS             3049..3429
                     /locus_tag="ACHUU3_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR2084033.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            3426..>4150
                     /locus_tag="ACHUU3_004114"
     CDS             3426..>4150
                     /locus_tag="ACHUU3_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR2084034.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRAS"
BASE COUNT         1116 a          914 c         1152 g          968 t
ORIGIN      
        1 atcaccgaaa aaaattggct tttacaccat tggcgataaa agcatttacc tgtatgacct
       61 acgccgcatg gatgaaatca tggaggctct tgataatcgt tcgtcgatgg attggtgtgt
      121 tgctgttcat gatatgaatg cagggtttga tgaaaagatt ttgttcccct catcagttga
      181 aagcactgcg ggttaaggag taacacatga ccactattac caaagaacgt attgaattgt
      241 tcattaaaaa cccgcttgaa aacgggctta cccgtggtga acaaatggaa ctggcacgga
      301 ttgcgctggc atcgctggaa gcagagccgg ttgtgttctg gtttgaaaaa tatcaagaag
      361 gggctacggc atgacgactt ttaccagaga gcagttaata gctcacgcag aggagactat
      421 tgaagcacag agactgtgca taccgggcac aatcgaccat gacatcatcc gcacatataa
      481 gatggatatt gctgttctgg aaatcgcact ggtatcgctg gcagcagagc cagccggtaa
      541 attgcatgaa tacaaaccag tgggatatca gcgtctggtc gatgagttaa ccatgctggt
      601 aaagcagtta acctggcaac tgagaaaagc gaagccagac tgcaaattac cggataaggc
      661 gatgagttat ctggagcgga acggactgat aagcgtggag gatattttac gatgacctgg
      721 cctgaagcat taacaacggt aggaattgcg atggcggtgg cgctggtggt gtattcgatt
      781 tgccgctggg gataaaaacg gtttgcggga aaaggagagt taagtagaat tgcagcgggt
      841 gcttgaggct atctgtctca ggcatgaaca ccaaaaggca gatagagaaa agccccagtt
      901 aacattacgc gtccggcaag acgcttaaca ttaatctgag gctcaatcta tgaacggcaa
      961 atctaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     1021 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac
     1081 gaggaaagac ctctgcgagg tacgaattcg aaccggccag acggaggtcg ctgtcttcac
     1141 agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc cacctctgat
     1201 gggtcaggca tcctcaatgc acccacactt aacccgcttc ggcgggtttt tgtttttatt
     1261 ttcaacgcgt ttgaagtttt agatggtgcc ggaatagaat caaaaatact taagtagcgc
     1321 gcagggagaa gagggatgga ccccgaacag gggagtgcta tttatctgga aggattctgt
     1381 tgatgaaaat cgaagaatta cgtgaaattt ttagtgaaga tggcctctat actgtgcgcg
     1441 ttgagaatgg cgctattgtc agccactgcc gtattaaatg tttacagtct caacaaagga
     1501 agagtggagc tgcgttaatt cattttgtgg atgggcttgt gacggatggt tttatttcgc
     1561 gtgcaaatga atttgtcaca tcgttgccgt ctctgaaaga agctgggatt aaggctggtt
     1621 tttctgcttt tgaagatgag tgaattcatc tacaattcag cgcagggctg aacccctgtt
     1681 gagtaacact gtgccaccgg agaaagccga tggcgcaaaa ttccagacta cacaattctg
     1741 ataattcagc cgtctttgcc agcaggcacg ggcggcgctc tcacgcattt aaatctgatt
     1801 ggtaccagca tcccccatgc actgaagaac aggccgaatg gctcattcag tgttaccgca
     1861 ggcgcggatg cgaggttaaa aaagccctta gcctcgacta ccgtcactgg ataatctccg
     1921 tcaggctccc ttactccgaa cggccaacgc gtccgtcccg cacattccag caacggatct
     1981 ggaggtaatg tgcgggtatt acttcgacct gttctggtac cggaactcgg cctggttatc
     2041 gttaagccag gccgtgaatc aatgtcagca ttccataacg gcagaatact ggtggagccg
     2101 gaaccaaaaa gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgctcg ccagccgctg
     2161 gtggaagaca aaacattgct gccgtttttc agtaacgcac gggtgattcg tgctgctggt
     2221 ggtgctggtg cattgtctga ctggctgttg cgccatatta aatcctgcca gtggccacac
     2281 ggcgattatc atcacagcga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg
     2341 tgctggcact gcgacaacca gctgcgtgac cagacatccg aatcactcga gcaacttgct
     2401 catcaaaacc tgtcagcatg gatgattgac gtcatcggtc acgcaataag cggtacgcag
     2461 gagcgtgaat tatctctggc tgaattatcc tggtgggcgg tccgcaatca ggtggcggac
     2521 gcgctaccgg aagcggtatt acgtcgttcg ctggggttgc gtgcggaaaa aattcgctct
     2581 gtgtacagtg aaagcgacat cataccggga gagcagacag ccaccagcat actgaagcag
     2641 cgcacaaaaa atattgcgtt actgcctcac gtccaccagc aacagaaccc accacaggaa
     2701 aagacggtgg tcagcattgc cgttgatccg gagtcaccgg ctcagtatct ccagcgccag
     2761 aaatcacaac gggaagagat gcctgtatac acgcgctggg taaaaacgca gaaatgcatg
     2821 acgtgtggca atcaggcaga tgatccgcat cacatcattg gtcatggact gggagggatg
     2881 ggaacaaagg ctgatgattt gtttgttatt ccgttatgcc gtaaatgccg taacgaacta
     2941 catgccggag taaaagattt tgaagagaaa cacggcagtc agctgttgtt gctgattcgt
     3001 tttttaatgc acgcgagaaa ttcgggtgtt ctgaagtgga aagcataaat gaccgagcgc
     3061 atagaatttg ttttacctta cccgccgacg gtgaacactt actggcgtcg tcgtggcagc
     3121 acatattttg tatcaaaagc cggggagcgt tatcgccggg cagtggcgct tattgttcgc
     3181 cagcagcggc tgaaattaag cctgtccgga aggttggcaa taaaaattat tgcagaacca
     3241 ccggataagc gccgccgtga cctggacaat attctgaaag cgccgctgga tgcgctgacg
     3301 catgcggggt tgctaatgga cgatgagcag tttgatgaaa tcaatatcgt tcgtgctcag
     3361 ccagtatctg gtggacgtct gggggtgaag atttatccca taatgcttga agggcaggtc
     3421 aaaaaatgaa actggaagat ttaccgaaat actactcccc aaaatccccc ggcctgactg
     3481 atgcatcggc ctcaacgtcg aaagatgcgc tgagtatcac tgatgtgatg gccgcgcagg
     3541 gcatgacaca gaatcgggct gagatggggg tttctgcgtt ccttgggaaa atgggcatta
     3601 gtatgaatga cagagagcgg gcaacagaat tgctgacaga atatgcactc agtcggtgtg
     3661 atcgcgtggc ggcgttaaga aaactcccgg cagaaataaa accggcagtg atgcgtatta
     3721 tggcttcgta tgcgtttgaa gattatgccc gtagcgcggc gagcaaaaaa cagtgcccct
     3781 gctgtcacgg aaaaaaattt attgaaagcg aggtttttac aaacaagatc cagtatccgg
     3841 atggtaagcc gccggtatgg gcaaagtgta cgaaaggtgt gtatccgtct tactgggaag
     3901 aatggaaaaa agtcagggag gtggtaaaag ttgcctgtcc ggagtgtggc ggaaagggtg
     3961 aggtttccac cgcctgtaag gattgccgtg ggcgtggtgt cgccattcat cgtgaagagt
     4021 cggtaaaacg tggtatgcct gttatcagag actgccagcg ttgtggtggt cgtggctatg
     4081 aaagactacc atcaacggag gcatttaatg ctatatgcga ggtgacaaac cagataacac
     4141 gcgcgtcatg
//
LOCUS       DAZPLI010000244         4091 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZPLI010000244 DAZPLI010000000
VERSION     DAZPLI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.247"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            110..718
                     /locus_tag="ACHUU3_004115"
     CDS             110..718
                     /locus_tag="ACHUU3_004115"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR2084035.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            816..2207
                     /gene="selA"
                     /locus_tag="ACHUU3_004116"
     CDS             816..2207
                     /gene="selA"
                     /locus_tag="ACHUU3_004116"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR2084036.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEIIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2204..4048
                     /gene="selB"
                     /locus_tag="ACHUU3_004117"
     CDS             2204..4048
                     /gene="selB"
                     /locus_tag="ACHUU3_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR2084037.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          920 a         1035 c         1209 g          927 t
ORIGIN      
        1 accatgataa atatgtgatt tatttggaag taacaaacca tcaacatttt ctgccctttt
       61 tcccatcacc aggccatact gatatttttc ctcgcacaaa ggactcggca tgaaactcgt
      121 cggtagctac accagcccgt ttgtacgcaa actttctatt ctgttgttag aaaagggcat
      181 aactttcgaa tttattaatg aactgcccta taacgcggac aacggcgtgg cgcaatttaa
      241 cccgttagga aaagtgccgg tgctggtgac cgaagagggc gaatgctggt ttgattcgcc
      301 gatcatcgct gaatatattg agttaatgaa tgtcgctccg gcgatgttgc cgcgcgatcc
      361 gctggagtcg ttgcgggtgc gcaaaattga ggcgctggcg gatggcatta tggatgccgg
      421 gctggtatcg gtgcgtgaac aggcgcgtcc ggcggcgcag cagtctgaag atgaattgtt
      481 acgccagcgg gagaaaatca accgcagtct ggatgtgctg gaaggatatc tggtcgatgg
      541 cacactcaaa accgatacgg tcaatctggc gactatcgcc attgcctgtg ctgtcggata
      601 tctcaatttc cgccgcgttg cgccaggttg gtgtgtcgac cgcccacact tagtcaaact
      661 ggtcgaaaac ctgtttagcc gcgaaagttt tgctcgcacc gaaccgccaa aggcttgatg
      721 cgcgatatgt cctcctgacc catctcacgt tacaatccgt ggttatgtta aacgcccttc
      781 tccgtgtgag agggccttga tcagccaggt ttcctatgac aaccgaaacg cgttccctct
      841 atagtcaact tccggctatt gatcgcttat tgcgcgatag ctccttcctt tctttgcgtg
      901 atacttatgg tcacacccgc gtggtggaat tgttgcgtca gatgctcgac gaagcgcgag
      961 aagtgattcg tgacagccag acgcttcctg cgtggtgtga aaactgggcg caagaagtcg
     1021 atgcccggtt gacgaaagag gcgcagagcg cgctgcgtcc ggtgatcaac ctgacgggaa
     1081 ccgtgctgca taccaacctt ggacgagctt tacaggcgga agccgcggtg gaagccgttg
     1141 cgcaggctat gcgttcgcca gtgaccctcg agtacgatct ggacgacgcc ggacgcggac
     1201 atcgcgatcg ggcgctggcg caactgctgt gccgtattac gggggcggaa gatgcctgta
     1261 tcgtcaataa caatgcggcg gcggtgttat tgatgttggc ggccactgcc agcgggaaag
     1321 aggtggtagt atctcgcggc gaattggtgg agattggcgg cgcgtttcgt attcccgatg
     1381 ttatgcgtca ggcaggctgc accctacacg aagtagggac caccaaccgc acgcacgcga
     1441 atgattatcg tcaggcggtg aatgaaaata ccgcactgtt gatgaaagta cataccagta
     1501 actacagcat tcaggggttc accaaagcga tagatgaagc ggaactggtg gcgctcggca
     1561 aagagctgga tgttcccgta gtgactgatt taggcagtgg ctcgctggtc gatcttagcc
     1621 agtacggttt gccgaaagag ccaatgccgc aggagttgat tgcggcgggc gtcagtctgg
     1681 tgagcttctc cggcgacaag ttgttaggcg ggccgcaggc agggattatc gttggtaaaa
     1741 aagagataat cgcccgcctg caaagccacc cgctgaagcg tgcattacgc gcggataaaa
     1801 tgaccctcgc ggcgctggaa gccacgttgc gtctttattt acaccctgaa gctctgagtg
     1861 aaaaattacc gaccctgcgc ctgcttaccc gcagcgcaga ggtcattcaa atccaggcac
     1921 aacgtttaca ggctcccctt gccgcacatt acggcgcgga gtttgcggta caggttatgc
     1981 catgtctttc gcagattggc agtggttcgc tgccggttga tcgcctgccg agcgcggcat
     2041 taacgtttac accccatgat ggacgcggta gccaccttga gtcattagcc gcccgctggc
     2101 gtgaattgcc agtgccgatg attggtcgta tttatgacgg acgattgtgg ctggatttac
     2161 gctgccttga agatgagcaa cggtttttgg agatgttgtt gaaatgatta ttgcgactgc
     2221 cggacacgtt gaccacggca aaacaacctt attgcaggtg attactggcg taaatgctga
     2281 ccgtctgccg gaagaaaaaa agcgcggcat gaccatcgat ctcggctatg cctactggcc
     2341 gcagccggat ggtcgcgtgc ctagttttat cgacgtaccc ggtcatgaaa agtttctttc
     2401 caacatgctg gcgggcgttg gtggtatcga tcacgcgctg ttggtggtgg cgtgcgatga
     2461 cggcgtgatg gcgcagaccc gtgagcatct ggcgattttg cagctgaccg gtaacccgat
     2521 gctgacagtg gcgctgacca aagccgatcg cgtggacgaa gcgcgtgttg atgaggttga
     2581 acgccaggta aaggaggtgc tgcgggaata cggttttgct gaggcaaaac tgtttatcac
     2641 cgcagcaacc gaaggtcggg gaattgatgc cctgcgcgag catctgcttc agttaccagg
     2701 acgtgaacac gccagccaac atagtgtccg cctcgcgatc gaccgcgcat ttaccgtaaa
     2761 aggtgccggg ctggtcgtca ccggtacggc gttaagcggg gaagtgaagg taggcgattc
     2821 actctggctg actggtgtaa ataaaccgat gcgtgtacgt gcgctgcatg cacaaaacca
     2881 gccaacagaa accgcccatg ccgggcagcg tatcgcgctt aacatcgcgg gtgatgcgga
     2941 aaaagagcag attaaccgtg gcgactggct gcttgccgat gcgccgccag agccgttcac
     3001 acgggtgatt gtcgagcttc aaacccatac gccgctgacc cagtggcagc cgctgcatat
     3061 tcaccacgcc gccagccacg ttacgggacg cgtttcgctg ctggaagata accttgccga
     3121 gctggtgttc gacacgccgc tgtggctggc ggataacgat cgcttagtgc tgcgcgatat
     3181 ctcggcccgc aacacgctgg cgggggcgcg tgtggtgatg cttaacccgc cgcgtcgcgg
     3241 taaacgtaag ccggaatatc tgcaatggct ggcgtctctt gcacgggcgc agagcgatgc
     3301 cgatgcgtta tctgttcatc tggaacgcgg cgcggttaac cttgcggatt tcgcctgggc
     3361 gcgccagctc aacggcgaag ggatgcgcga attgctgcaa cagcctggct atattcaggc
     3421 tggttatagc ttgttgaatg cgccggttgc cgcccgctgg cagcggaaaa ttctcgacac
     3481 attagcgact tatcatgagc aacatcgcga tgaacctggt cctgggcgcg aacgtctgcg
     3541 acgtatggcg ttgccaatgg aagatgaagc gctggtactg ttgctgattg aaaagatgcg
     3601 cgaaagcggc gacatcctca gtcatcacgg ttggctgcat ctgccggatc acaaagcggg
     3661 cttcagcgaa gagcagcagg ccatctggca aaaagcagag ccgctgtttg gtgacgagcc
     3721 gtggtgggtg cgtgatctgg caaaagagac gggtaccgac gagcaggcaa tgcgcctgac
     3781 gctacgccag gcggcgcagc aaggaataat taccgcgatc gtcaaagatc gttattaccg
     3841 taacgatcgg attgtcgaat ttgccaatat gatccgcgat ctcgatcagg agtgtggttc
     3901 aacctgcgcg gcggatttcc gcgatcgctt aggcgtaggc cgaaagctgg caattcagat
     3961 tctggaatat tttgaccgca ttggctttac gcgtcgtcgt ggaaatgatc atttattgcg
     4021 cgacgcatta ttatttccgg aaaaataagg aaatgattaa ttaattttta aaataaatta
     4081 atacaaattt c
//
LOCUS       DAZPLI010000245         4087 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZPLI010000245 DAZPLI010000000
VERSION     DAZPLI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.248"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..877
                     /gene="adeQ"
                     /locus_tag="ACHUU3_004118"
                     /gene_synonym="yicO"
     CDS             <1..877
                     /gene="adeQ"
                     /locus_tag="ACHUU3_004118"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFR2084038.1"
                     /translation="GLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIIT
                     VLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELA
                     GIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTSS
                     VTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMT
                     SSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCV
                     VVVASLFALKIILVD"
     gene            930..1382
                     /locus_tag="ACHUU3_004119"
     CDS             930..1382
                     /locus_tag="ACHUU3_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFR2084039.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1593..2773
                     /gene="nepI"
                     /locus_tag="ACHUU3_004120"
                     /pseudo
     CDS             1593..2773
                     /gene="nepI"
                     /locus_tag="ACHUU3_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2814..3107)
                     /gene="yicS"
                     /locus_tag="ACHUU3_004121"
     CDS             complement(2814..3107)
                     /gene="yicS"
                     /locus_tag="ACHUU3_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFR2084040.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3329..>4087
                     /gene="nlpA"
                     /locus_tag="ACHUU3_004122"
     CDS             3329..>4087
                     /gene="nlpA"
                     /locus_tag="ACHUU3_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFR2084041.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFLHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQS"
BASE COUNT          899 a          906 c         1077 g         1205 t
ORIGIN      
        1 tggattattt atcgccttaa tgggattaaa aaatactggc gttattgtcg ccaataaaga
       61 cacgctggtg atgattggcg atttaagttc tcacggcgtg ttgttaggta ttttagggtt
      121 ttttattata accgtgttgt catcacgtca ttttcatgcc gcggtgctgg tttctattgt
      181 ggtgacgtct tgctgtggat tatttttcgg tgatgttcat tttagcggcg tctattccat
      241 tccgcctgat attagcggcg tcattggtga agtagatttg agcggcgcgt taacacttga
      301 actcgccggt atcattttct cctttatgct gatcaaccta tttgattcat caggaacatt
      361 aattggtgta actgataaag cgggcttaat agatggtaac ggtaaattcc ccaatatgaa
      421 taaggcgctg tatgttgata gcgtcagttc ggtggcgggt gcgtttatcg gcacctcgtc
      481 tgttactgcc tatattgaaa gtacttctgg tgtggcagtc ggtggccgca cggggctgac
      541 tgcggttgtg gttggcgtta tgttcctgtt ggttatgttc ttctccccgc tggtggcgat
      601 ggttcctcct tacgcaaccg ccggagcgtt aatctttgtt ggcgtgctga tgacttcgag
      661 cctggcgcgc gttaactggg atgattttac cgaatcggtg cctgcgttta ttaccacggt
      721 gatgatgccc tttactttct cgatcaccga agggattgca ctcggcttta tgtcgtactg
      781 catcatgaaa gtgtgcaccg ggcgctggcg cgatctgaac ctgtgtgtgg tggtggtcgc
      841 atctctgttt gcactgaaga ttattctggt ggattagtaa ctggcgaggg tttccagata
      901 tcatgagttc tgataacgca ggagaactca tgatctggat aatgctcgcc acgctggcgg
      961 tagtgtttgt ggttggtttt cgggtgctga catccggggc cagaaaagcg attcgccgtc
     1021 tcagtgatcg gctgaatatc gatgtcgtac ccgtggagtc gatggtcgat caaatgggaa
     1081 agtcagccgg tgacgaattt ttacgttatt tgcatcgccc ggatgagtcg cacctgcaaa
     1141 acgccgcgca ggtgttgctc atctggcaaa ttgtcattgt cgatggtagc gaacagaacc
     1201 tgctgcaatg gcatcggatt ttacaaaaag cccgcctttc cgcgccgatt accgatgctc
     1261 aggtcaggct ggcgctgggt tttctgcgcg aaaccgaacc tgaaatgcag gatattaatg
     1321 cttttcagat gcgctataac gcgttctttc agcctgccga gggcgttcac tggctgcatt
     1381 aaaaccgctt cgtcttatca gcctacagac cgcgcctgac ctgtcgggtg gataagacgt
     1441 tcacgccgca tccggtaatc tttgccctgt tccgtttcga tcttaactcc agctcaagca
     1501 acaccgtaaa agttgcaaaa acgttaaatg cgtcacacat ttcaatgtta aagtggtcgg
     1561 cttttcccct gaaacatgcc acgggtaaca ccatgagtga atttattgcc gaaaaccgcg
     1621 gcgcggatgc catcacccga ccgaactggt cagccgtttt ctcggtggcg ttttgtgtcg
     1681 cctgtctgat tatcgttgag tttttgcccg tcagtttgtt gacgccaatg gcccaggatt
     1741 taggcatttc ggaaggggtt gccgggcaat cggtgaccgt gaccgccttt gtggcaatgt
     1801 ttgccagttt gtttattacc cagacaattc aggctactga ccgccgcaac gttgttattt
     1861 tgtttgccgt tttgctgacg ctctcctgct tgctggtttc ctttgctaac tcattcagtt
     1921 tgcttttaat cggtcgtgcc tgtctggggc tggcgctggg cgggttctgg gcgatgtcgg
     1981 cgtcgctgac catgcgtctg gtgccgccgc gtacggtgcc gaaggcgctg tcggtgatct
     2041 tcggcgcggt ttctattgcg ctggtgattg ccgcgccgtt gggcagtttt ttaggcgagc
     2101 ttatcggttg gcgcaatgtc tttaatgcgg cggcggtgat gggcgtgctg tgtattttct
     2161 ggattatcaa atcattgcct tcactgccag gcgaaccctc gcatcagaaa caaaatactt
     2221 tccgcttatt acaacgtccg ggtgtgatgg cagggatgat cgccatcttc atgtctttcg
     2281 ccgggcagtt tgctttcttc acatatattc gcccggtgta tatgaacctg gcgggattca
     2341 gcgtggatgg cttaacgctg gtgctgttga gttttggcat tgccagcttt atcggtacgt
     2401 cgctttcgtc attcattctt aaacgttcgg taaaactggc cttagcaggc gcgccgttaa
     2461 tactggctgt gagtgcgttg gtgctgacgt tgtggggaag cgataaaatc gttgctaccg
     2521 gcgtggcgat tatctggggg ctaacttttg cattggttcc cgtcggctgg tcaacgtgga
     2581 tcacccgctc actggccgat caggcagaaa aagccgggtc tattcaggtg gcggttattc
     2641 agcttgtggc gcggcaatcg gcggttatgc gctggataat attggtctga cttcgccgtt
     2701 gatgttttct ggcacattga tgttgctgac tgcattgttg gttactgcaa aggtgaaaat
     2761 gaagaaatcc tgatatcagg taaagaagcg ttggtcagta gttgttgctg ttcttacata
     2821 tccgggcatt ctaccttacc caacgcttcc caataattgc tctgattatt acgttccatc
     2881 gccacgatgg cctcgcgtat atgttgttta ttgttttcat ctgacaacat taacttttcc
     2941 cacgcttcat ctgataatga cgcctgtggc gtgcaaattt tacgtaaatc ttgtaatacc
     3001 gtggttttct cttttgcgga ctgcagtgag ctaaaaggcg actcggcgta tacgatgcct
     3061 ggcatagcga aaaaagtgaa aataagcagt agtgtcgttg gtttcatgga ttcaaccttt
     3121 tacaaatgtg tatcgaatga agcataagtg tagtttgctt tcatcgctct catcatgctg
     3181 tacgaaacgc gcttgcgtgg tcagtaagaa gtgccagact ttatattcca cttttattcc
     3241 tttttattct tatcgatagc gtttcgtttt ttaaaccgca gcgaccttac cgctatagtc
     3301 aggtaatcat taataaaagg ataaaaaaat gaaactgaca acacatcatc tacggacagg
     3361 ggccgcatta ttgctggccg gaattctgct ggcaggttgc gaccagagta gcagcgatgc
     3421 aaaacacatt aaagttggcg ttataaatgg cgcagaacaa gatgtcgcgg aagtcgctaa
     3481 aaaggtggca aaagagaaat atggcctcga tgttgaactg gtaggattta gcggttcgtt
     3541 gctaccgaac gatgcgacaa atcatggcga acttgatgcg aatgtcttcc tgcatcgacc
     3601 ctttcttgaa caagataatc aggcgcatgg ttataaactg gtagcggtgg gaaatacgtt
     3661 tgtcttccct atggcgggtt attccaaaaa aatcaaaacc gtggcgcaaa taaaagaagg
     3721 cgcgacagtg gcgatcccca acgacccgac caaccttggt cgcgcacttt tactgttgca
     3781 aaaagagaag ctaattactc tgaaagaggg aaaagggtta ttacctaccg cgctggatat
     3841 aaccgataat cctcgtcatt tgcagattat ggaactcgag ggggcgcagc tcccgcgcgt
     3901 actggatgat cccaaagttg atgtagcgat tatcagcacc acttacattc agcagacagg
     3961 gctttctccg gtgcacgaca gcgtatttat tgaagataag aattcgccgt atgtgaatat
     4021 tttggtggca cgggaagata ataagaatgc agaaaacgtg aaggaatttc tgcaatctta
     4081 tcaatca
//
LOCUS       DAZPLI010000246         4077 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZPLI010000246 DAZPLI010000000
VERSION     DAZPLI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.249"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(46..753)
                     /locus_tag="ACHUU3_004123"
     CDS             complement(46..753)
                     /locus_tag="ACHUU3_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR2084042.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVAS
                     PELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQY
                     RAQYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRNYSIRIYEILQQFRS
                     TGERIIALDDFKSSLMLEGKYKDFKTLNRDLIKPCVDELNKKSDLAVTVETIKKGRTV
                     VALHFRFKEDKQIKMTI"
     gene            complement(1286..1540)
                     /gene="mobC"
                     /locus_tag="ACHUU3_004124"
     CDS             complement(1286..1540)
                     /gene="mobC"
                     /locus_tag="ACHUU3_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="HFR2084043.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRK
                     PEEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
     gene            1719..3866
                     /gene="mobQ"
                     /locus_tag="ACHUU3_004125"
     CDS             1719..3866
                     /gene="mobQ"
                     /locus_tag="ACHUU3_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFR2084044.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTR
                     KSGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTH
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETE
                     RGEQNRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEAL
                     PKDSNAAWKLTSNAMSMMADMQATRRCWEELNTERKEAMSHAASLKKSSPFSSGFSRI
                     PLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGESGL
                     AALRAQLLKREEELRRKELEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGN
                     GEMTCYLLLHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQHPPLQR
                     VYSSATFEQLLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQG
                     RLPPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVI
                     SALTDKGLRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLF
                     RSLGFNRTWDEWRREEDARTHTTERRHDRGWSQ"
BASE COUNT         1050 a         1046 c          995 g          986 t
ORIGIN      
        1 aaaaaaaacg cagcagggag cgttttaggt gccttaaaat cgattctaga tcgtcatctt
       61 gatctgtttg tcttccttga agcgaaaatg cagggctaca accgtgcgcc cctttttgat
      121 cgtctcaacc gttacagcca ggtcgctttt tttgttcagt tcgtcaacac agggttttat
      181 tagatccctg tttaacgttt tgaaatcttt atatttacct tcaagcatca gtgatgattt
      241 aaaatcatcg agggcgataa tacgttcccc agtactgcgg aattgctgga gaatttcgta
      301 tattcttatg ctgtaatttc ttgataaatt ggaaatattt ttaatgacaa ctctggtgaa
      361 ttgccccttc aactgtgtca gataaggcat taccgcgtca gaaaaagtga tttgtgcttt
      421 cccctctccc ctcgcgtact gtgcccgata ctgaatccag cggaactcct cgcgcttctc
      481 gtcgtccttg agaatgatcg acctgtccca caaacgatcg atcgcttcct gtagctgaac
      541 ctcggcattt ttccgcccca tatcagggaa tgaatcaaaa tattccgcag ctgtaatggt
      601 cattgtcttt tgcaattcag gtgaagctac gtcagtaccg gagttaagtc ggcttatgca
      661 gagcaataag aaacgctgtt cttgtaaagt tagcttgtaa ctggcctcaa ccagcgcatt
      721 ggacttatag gctatcagag ggtttgatgt cattttaacc accaggctaa aagtgaggtg
      781 agataaaaac ctatcacctt tagcattggt gagcaattat atttctaccc cccattttcc
      841 tgtgaatatt accttaaatc tcctcacctt taccttaaat tacctcacct aagcacctta
      901 aattatatca cctttacctt aaatctcccc acctatagac gcgtaacgta ttgtttttaa
      961 tcaggaattt cgaccctaaa acaagtatta aaaacaagat cttaaaaaca agatctaaaa
     1021 atttaacggc gagcctgtgg ataacttggg gcgctgaaaa taaaaatcca ataaaaacaa
     1081 tgctttgcaa aaaaaccagg attgaatatt ccgaatacac cccctccaac cctgtggaaa
     1141 cccagtattc atgcgggttc agcgcatatc acctgccgaa aaaccgtgcg tatgtgctca
     1201 acctggcgac ggattcactt ccgcttcctc gcccactcgc tcgctaacgc tcggtcgctg
     1261 gggctgcggc gagcggacga tacaatcact gtcctggcag cgcctccagc tcgttaatca
     1321 gcggcagtat gcgcgtcaca tcagcctgtc gtgtcacctc tgccttcagg aactggagta
     1381 accaggcacg caactgcacc tcctccggct ttctgacctt tgccagcaat gctgcaccca
     1441 caacaacctt ctgcgctgtg tccaggctcc ggctcttcgc cttcaggcgc tgtaatctcg
     1501 cctcagcatc agcaatcttc tgttcgagcg ttctgctcat gatgtaactc cgtgcgcgat
     1561 gaaaaatcgc attttagcgc gtcactggta gtttaaaaac tgagctggca taatgcacgg
     1621 caaatcacga agtgcgcact tatacaatct ccactccgtt tcgattgtgt gcgccctgcg
     1681 gggctgaaag aaaactacaa aaaggcatta cggcagaaat ggcgatttat catctcagca
     1741 tgaaaattat ctccagaagc agcggctaca gcgccgtagc ttccgctgcc tatcgttccg
     1801 gttcactgat gcttgatgag cgtaccggac tgactcacga ctacacccgc aaaagcggtg
     1861 tggctgaagc tgtgattctg acccccgcga cagcccctgc ctggtgtacg aaccgtgccg
     1921 aactctggaa tgccgttgag aaggccgaac gccggaagaa ctcacaactt gcgcgggaaa
     1981 ttgaactcgc tatcccccgt gaactgccgc aggatgccgc acgggaaacc gttctcgcct
     2041 ttgtcagaga aaactttgtc agtcagggca tgattgctga tgtggcgttc catcacatgg
     2101 ataaaacaaa tccccatgcg cacatcatgc tgaccacgag ggctgtcgga cctgctgggt
     2161 tcggcggaaa ggtcagggac tggaacgacc ggacacatgc cgagacgtgg cgtgcatcat
     2221 gggctgacca tgcaaacaga gcactggcga acgccggtta tcaggaagag atagaccatc
     2281 gttcatacga gcgacagggg cttgagaaaa cgcctggtat tcacctcgga aagtctgcct
     2341 gtgcgatgga aacacgcggt atcgaaacag aacgcggcga acagaatcgt ctgattaaca
     2401 ggcttaacct ggaaatacag atttcccgca ctgaactcag gaacagcggg ctgacagagc
     2461 ctgcccgccg tgtggctgac cttctgaata tcgtgcttcc tgacaacgca acttcgtaca
     2521 cactacgtga catcatcgaa gcgttgccga aggacagtaa tgcggcatgg aaactcacca
     2581 gtaatgccat gtcgatgatg gcagatatgc aggcgacgcg cagatgctgg gaagagctga
     2641 acaccgaacg aaaggaagcg atgagccatg ctgccagcct gaaaaaatca tcgccgtttt
     2701 cgtccggttt ttcccgtatt cccctgatgc aatgggcggc accggaatac cgtagcgaac
     2761 agggaaaaat ccagtcgctg agccagcaaa tggaaaaatt gcgacagcat taccggaagg
     2821 tggagaaaca ggacattccc gcgagtcaga cggcatttga aaaacagtgg cagcagtggg
     2881 gggaatccgg ccttgcagct ctcagagccc agttgcttaa acgtgaagag gaattgcgcc
     2941 ggaaggagct ggaggataca gaacgtagtc gcgcggagca gctcaggaaa aatgacaatg
     3001 ccgttatgga ttcaggggtg ctgagagcag tcgttacggg gtatggcgag gctcccatgc
     3061 ctggtaacgg ggaaatgacc tgttaccttc tgcttcacaa ccggaacgga gaatatacgc
     3121 tctgggggaa cgagctggag aaataccgca ccaggatctt tgagagtgtt gatttaatgc
     3181 gtgacaggag tggttatatc tgtgaccgaa gcgaaatcgg tcaacatcca ccactgcaac
     3241 gtgtttattc ctctgcgacc tttgagcaac ttctgacgca ggtttgccag acatggccgc
     3301 aacatactcg caatctccgc cagccgaaga catggccgga gtctttctgt cttggagaag
     3361 accgccagcc agccatgcca tcactggcag caaggaaggt ggattttacg cagggacgtc
     3421 tgccaccgac gctgatgccg gtcatgagtt ctgtcgaccg ggaaacccgc cagcttcagc
     3481 ttttgctggt catgggcgtg gatgactctc tgggcggtgt tgtccggctt aatggtacgc
     3541 tgtatcccgc gtttgccgtt ccctcagccg ataacagcca gctggtcatc agtgcactga
     3601 ccgataaggg gttacggtat gcagggtatg gtgttgccgt aaatcatgat gcagacagcc
     3661 acatatcacc tgcgccggaa cttatggagt ttcatctgaa aacccgtgag gcaccgcttt
     3721 ttgctgccgt caacacacca gaaaaacagc cggatcatct gttccgctcg ctgggtttca
     3781 atcgtacctg ggacgaatgg cggcgcgagg aagatgcaag aacacacacc accgagagga
     3841 gacatgatcg cggttggtca cagtgaatgt gaacaatatt ttgttttgtt taacatcgtg
     3901 ttatgttctt cgatgttctt cgatgttcat cgacagggat gaatgcaaga agcacactca
     3961 cataacgcaa gcatagtttt gtttttccgc ccccaaaatt gggggttctt ttttcgttgc
     4021 atcagagcgt ttttgacgca atttttcact tcaatagtac cttcgcacta ctaacac
//
LOCUS       DAZPLI010000247         4075 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZPLI010000247 DAZPLI010000000
VERSION     DAZPLI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.250"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..784
                     /locus_tag="ACHUU3_004126"
     CDS             <1..784
                     /locus_tag="ACHUU3_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR2084045.1"
                     /translation="AKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVN
                     QQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLS
                     DTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQG
                     NRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGS
                     RLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            797..1756
                     /locus_tag="ACHUU3_004127"
     CDS             797..1756
                     /locus_tag="ACHUU3_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR2084046.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGRSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(1794..2693)
                     /gene="nhaR"
                     /locus_tag="ACHUU3_004128"
     CDS             complement(1794..2693)
                     /gene="nhaR"
                     /locus_tag="ACHUU3_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR2084047.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2759..3925)
                     /gene="nhaA"
                     /locus_tag="ACHUU3_004129"
     CDS             complement(2759..3925)
                     /gene="nhaA"
                     /locus_tag="ACHUU3_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR2084048.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1224 a          952 c          861 g         1038 t
ORIGIN      
        1 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
       61 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      121 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      181 gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag
      241 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
      301 gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg
      361 acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca
      421 tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg
      481 caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga
      541 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
      601 ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct
      661 ttttattcgc gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa
      721 cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
      781 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
      841 tgtgcaaagt gctccctgtg cattgaccaa tgtcggggaa caacgtggaa catatatact
      901 caagccactg agtatgaaag gtaatctcac cgccgaaaaa attgaaaaca ccggaaatat
      961 cattgagtgt gcagacttac aagagacgac caacgccatt aaagttcatt ttttaacaca
     1021 gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc
     1081 tgacggtaaa aaaatagagc ttccaggaag gtcatcttat aatttagaag aaatgctatc
     1141 tgcgataaat ttcaccattc catactctat cgcatctgta tcagatagtt ccaacaccgc
     1201 agatattttt ccaggaactc cttttccttt gttgttcgaa attgaagtga gttcatgtac
     1261 agggaataat actaattgta atagcgtccg ctttaattat aatttaaata ctattttaca
     1321 ggtaaattta atgacctgtg gttttgaaga tcaatccatc gacactggac gatttgtact
     1381 gtctcgtatc aataatgaaa attatcagtt tcatcatata agatttgact gcatccaggg
     1441 cgagcaaggt gaattagttt ttgaacccag tgatgttgaa tattactttg aaccagtgac
     1501 gatacaggag tcaggaacca gcatattaaa aaatgagtta gaaaacagtg aggacggtgc
     1561 gggtcaagta ggctttcaac tctccaatga tggtacgaat gaaatccagt atggtaaaag
     1621 caattactac tcttttcagc atccccatga aggaagtaat cagattcctc tatttattcg
     1681 cccgcgtaca tatggaaata atgtctcaag cgggcaaata atgagccgag tcaaaatcgt
     1741 ggttatgtac aactaatcta tctcccccac cttaactttg ggggaggtgc cgattaacgc
     1801 gccgctggac taaaaagcgc agaataatcc gtattgcaga ttcgctgtac cgccgggtgc
     1861 tgaatcatcc gctcagcaaa aatagcatgg tactcttcca tcacattctc gacgcgacca
     1921 atttctacga cagttttatc ggcataaaag tcatatgcat aaagcgttgg ggcaacgaag
     1981 attgcattgt gcatcgcacc aaaagctttc atcaacgcgg catcatcaaa ctcgccgagg
     2041 atttctacgt ttaatccctg ggagttaaac cagttaagca atttgcgccc taacattgaa
     2101 cgtcgcccag gaatcaaaag tcgccgttct tccagacaag ccgggaacga tttttctggt
     2161 ggtggatttg tacaccagaa actcacgcca cattcgccaa ttctcacgga gaacaggcct
     2221 tcctgctgcg tagagtctat cggacagtca gaaatgatca tatccagttt atgctgactt
     2281 aattgctcca gcagcatttc gtgggtggat tcgaagcagc gaagatgaat gggctcgcct
     2341 tctaccactg cggcgttaag tacgctactg accaggcgtt tggaaagtgc atcagccacg
     2401 ccaacgtcaa acaataaatt ggattctttg cgatagttca caatatccag catttcctgg
     2461 cttaaggtga acattttatc ggcatagcga tagaccagtt ctcccagctc gctgggttcg
     2521 agaccacgtc ccttgcgttt aaataatttg ccttgcaggc gctcttccag cgctcgaatc
     2581 tgtccggtaa tggtttgtgg agttaaataa agcgcctccg ctgcgccaac cacggaacct
     2641 tctttataga catgccagaa gtaatacaag tggttgtaat tgatatgaga catgctcatt
     2701 tctctccctg ataacaatga aaagggagcc gtttatggct ccccggtaaa ccgtcctgtc
     2761 aaactgatgg acgcaaacga acgcgtaacc agctgtatcc aattaccgcc gaagagatag
     2821 aaccgaccag gataccgagt ttcgcccagt taatcagttc tggatctacg ctaccaaagg
     2881 ccaggctggc aataaagata gacatagtaa aaccgatacc gcacaggatc cccaccgcca
     2941 taatttgctg ataagtcgtt ccctcaggca gatgcgccag tttcaaacgc agcgccaacc
     3001 agcagaacag actaatcccc agcggtttgc caatcagcaa gccagcgatg atccccaatg
     3061 gcagaatgga ggtcaagcca tccagcgtga cgccttgcag tgaaacgcca gcattagcaa
     3121 atgcaaacag cggtaaaatc agatacgcca cccacgggtg caatacatgc tccagtcgct
     3181 tcgctggaga acgcccatgc ttctctttca aaggaataaa gaagccgaca attacccccg
     3241 ccagggttgc gtgaaccccc gatttcaaca ccgcagtcca caacaccacg ccaacaagaa
     3301 tatagacgcc cgtgcggcgt acaccacaca gattcaatac cgcgagtacc gcaattgcta
     3361 cagccgcgac gccaagagag gccatcgata agtcattagt gtagaacaat gcgatgataa
     3421 tgatggcccc aagatcgtcg ataatagcca gagccatcaa aaagatcttc agtgctaacg
     3481 gaacacgact tcccaacagc gccagcacac caagtgcaaa ggcaatgtca gtagccgccg
     3541 ggatcgccca cccttcgcgg gtaatcggat cggcatagtt aaaggccaga tagagtaatg
     3601 ccggaacaat catcccacca atagcggcga taaccggaaa tgcggcctgg cgtaagctgg
     3661 ctagcgatcc ttgcatcagt tcacgtttaa cttccagacc gactaacagg aaaaataccg
     3721 ccatcagcgc gtcatttatc cataacagca tgtttttgtt gatttcgagt gaaccaaccc
     3781 ggagctgaac cggcgtctcc agaaagtcgt gataccatcc actggttgcg ccgctgttgg
     3841 ccatcatcat cgccaggata gcggcaatga taagaataat gcctcccgag gcatcactgc
     3901 taaagaatcg atgcagatgt ttcacttttt atttctcttt caggtgaata gatcgttttt
     3961 actattttac cccgcacaaa tcatcgttaa aatcagatta tagtgtagga ataactcgtt
     4021 ttttacgagc aattaaagga tttgcgccat cagcggtgag atgagttttt gacgg
//
LOCUS       DAZPLI010000248         4061 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZPLI010000248 DAZPLI010000000
VERSION     DAZPLI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.251"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..784
                     /locus_tag="ACHUU3_004130"
     CDS             <1..784
                     /locus_tag="ACHUU3_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR2084049.1"
                     /translation="FSVSAPGHHSWKRDFDPDSPEIKFSDRREALKLMTQIESTVTSL
                     HREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEGFTVKITFPAVSQ
                     LQTRIAVKTNLSALMEERTETVTRRRRQSGFWGKICGAFGTSDWGWENYKENVSRSVI
                     NINSVRKEVMSLTRAYFGELQASIEQNINQPVRQEIDDFFCTFREKVEQLRNTLIQSS
                     EDHKRDQQAQEQLTERLQALNERVPELITDSKALREELETLL"
     gene            781..1686
                     /locus_tag="ACHUU3_004131"
     CDS             781..1686
                     /locus_tag="ACHUU3_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR2084050.1"
                     /translation="MTSPFIQQIADNRVCQVLSCLPEKFVVDFANGIDVAQEHNRTAG
                     GRTFFRRLKEGLTGKGAVRQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLAEQVHQKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     NGGSATVRLNTLDWLGGQSREQADNEWHEAVNWLGDWCSEERHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGEA"
     gene            1683..2753
                     /locus_tag="ACHUU3_004132"
     CDS             1683..2753
                     /locus_tag="ACHUU3_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFR2084051.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGAQISMVSGASIG
                     ALNGAIITASPDLSEAALRLEALWDHLGNNQVLSVNRSVYFSLLKKLVQAMNLCQIPG
                     RAGALLTTLFRHISTINGFDNPMIQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMYGDGGMGGWRNRQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRRAYPDTTILEIRPR
                     KRLKQIGDEGKSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALTRSETVL
                     QKSLEITEGADSALRNAMARIK"
     gene            2990..3343
                     /locus_tag="ACHUU3_004133"
     CDS             2990..3343
                     /locus_tag="ACHUU3_004133"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084052.1"
                     /translation="MFSIGNVSINCTINPEDNNLLPHYVWIYGHTSTQLNQYDSDIIF
                     IINDKKYPAPSVDGTRMNKNAWVYFIDAISEATQFDVLVNGKKVDSYTANIKNVKKTL
                     GNKFYDSCWNTWFQE"
     gene            3469..4008
                     /locus_tag="ACHUU3_004134"
     CDS             3469..4008
                     /locus_tag="ACHUU3_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HFR2084053.1"
                     /translation="MNWHYEKNGVRYDNVTEEDIIERIRRGELNASTLVWQQGMLEWQ
                     PVSATSLAEILKQSEVPPALPGHRIPGGVVWTLAFAPLIGYILEMWTAGLNGMSFERA
                     YDAVSEGQYWFISLILNIALGYLDERKLRKSGVDTATFGWLAWLIPVYLWRRAKILGQ
                     KPAYFWVWLVMLILVLFTV"
BASE COUNT         1077 a          900 c         1101 g          983 t
ORIGIN      
        1 gtttagtgtc agtgccccgg gccatcattc atggaaacga gactttgatc cggacagccc
       61 cgagataaaa ttcagtgatc gcagggaagc ccttaaactg atgacgcaaa tcgaatcgac
      121 cgtgaccagc ctgcaccgtg aggctgaagc acagttccgg cctgaactgg aaaaaatcgt
      181 caggggtatc gaaacaggtt ttcgtggtac ggccctgtat gccacagaaa atattgccgg
      241 acgtatcaat gcccgcctgg aggacgaggg cttcactgta aaaatcactt tcccggcagt
      301 cagccagtta cagaccagga tcgcggtaaa aacaaatctg agtgcgctta tggaggaaag
      361 aactgagaca gtcacccgtc gccgtcggca gagtggtttc tggggaaaga tttgtggagc
      421 gtttggcacc agtgactggg gctgggaaaa ctataaggag aatgtgagcc gcagtgtgat
      481 caatatcaac tcggtcagga aggaagtgat gtcactgacc cgggcatatt tcggggagtt
      541 gcaggcatcc attgagcaga atattaacca gcccgtccgc caggagatcg atgatttttt
      601 ctgtacattc agggaaaaag tcgaacagct acgtaacaca ctcattcaga gctctgaaga
      661 tcataaacgc gatcagcagg cacaggagca gcttacggag cgccttcagg cattaaatga
      721 aagggttccc gagctgatta ctgacagtaa ggcgttaagg gaagaactgg agacactgct
      781 gtgacctcac catttattca acaaattgct gataaccggg tatgtcaggt actcagttgc
      841 cttcctgaaa aatttgtggt tgattttgca aacggtattg atgttgcaca ggagcataac
      901 cgcacggccg gggggcgcac gtttttccgg cgtttaaaag aaggtctgac tggcaaaggc
      961 gctgtacgcc agaatgccat caatgcttcg cttgcacagg gcgttgaggc gtccctgcgc
     1021 tggctgacgg agctgacaac gtcgcttgcc accacaaatt acgcgattac ccgggtaaac
     1081 gacagggtca gttcactggt cagtgatact gccaggctgg cgcattattc ggcagacacg
     1141 cgggagcagt tactcaccct ggcagaacag gttcatcaga aactgaatca tcttgaagaa
     1201 aaactccacc gtgttgacca ggttcagcgg gcacagttac atcttgagca gatattctca
     1261 tggtggagcg ccgggcgata cgcgtcattt tcccctgccg gacgttgtta tgtggcgctt
     1321 gaagagcttc gctggggcgc gtttggtgat gtgatacgtc agggtgaaac aggccaggtt
     1381 aaccagttac tggatatcct cagacataaa gcattaacgc aaatggcaca ggagaatggc
     1441 ggtagcgcaa cagtacgtct gaacacactg gactggcttg gtggtcagag ccgggaacag
     1501 gctgataacg aatggcatga agcggttaac tggctggggg actggtgcag tgaagagcgg
     1561 catcctgtga tctggtcaac cacacaggct gcagaacact taccggttcg tatgccccgt
     1621 ctctgctctg cagaacgcct ctctgaaagt atggttgatg aaatatttca gaagggggag
     1681 gcatgagtac agaaatgaaa acggggctgg tgctgtccgg cggcggggcg gtgggcgctt
     1741 atcaggcggg agtggttaag gcactggcag agtgcggcgc acagatcagt atggtctcag
     1801 gagccagtat cggggctctc aatggtgcca ttatcacggc ctctcccgat ctgtcagaag
     1861 ccgccttacg cctggaggcg ctctgggatc atctggggaa taatcaggtt ctgtcggtaa
     1921 acagatcggt ttacttttca ttgctgaaaa aactggttca ggccatgaac ctctgccaga
     1981 tccccggacg tgcgggcgca ctgcttacga cgctttttcg ccatatatcg acaatcaacg
     2041 ggttcgacaa tccgatgatt cagccgctgt tgtcagatga gcccctgaca gcgctgatgg
     2101 atcattatct tgatacagat gctctggccg acgggctacc gctgtacgtg tcgctgtacc
     2161 ccacagaagg aggcatgcag gatattattg actgcattcg tgctgaactg ggtgccggaa
     2221 ccacgaaaaa cgctgttttt cagcatatcc agagtctgcc ccgcgggcag cagaaagaag
     2281 ccttactggc gtcagccgcg ctgcctctgc tgttccgtcc ccgcgaggtt cagggaacaa
     2341 tgtacggtga tggtgggatg ggcggatggc gaaacaggca ggggaatact cccgtgacgc
     2401 cactggtgga tgccggatgc aatatggtga ttgtgacgca tctgagtgac ggttctttat
     2461 gggatcgtcg ggcttatccg gacaccacaa tccttgaaat ccgtccccgg aaaaggttga
     2521 aacaaatcgg ggacgaaggc aaaagtggcg gtctgctcag ttttacatcg gcacataccg
     2581 acgcctggcg tcagcagggc tacgaggaca cgatgctgac gatggagcat atccggaaac
     2641 cgctggcagc acgtcaggca ctgacccggt cagagacggt attgcagaaa agcctggaga
     2701 taactgaagg tgcagattcg gcactgagaa acgcgatggc ccggattaaa taaagacgct
     2761 ccggagaaac gccaccgcca ctgtggtggt gtttttcctg tctggtatta ttgaaaatat
     2821 tttcagatat atattacctg gatgatggtt aagattaatt tatttaattt tggggagaaa
     2881 actcatgaaa ctgttaaaca ttcttttagc tgttgtttta actgtaagtg caaccgtagc
     2941 acaaacagaa tcggcattgt agacgcgtaa cagtattgct ggcactgata tgttcagtat
     3001 cggtaatgtt tctattaact gcacgattaa tccagaagac aacaatttgc taccacacta
     3061 tgtgtggatt tacggtcata cctccaccca gctaaatcag tacgattctg atattatttt
     3121 tatcatcaac gataaaaagt atccggcccc atctgtcgac ggaacacgaa tgaataagaa
     3181 tgcatgggta tactttatcg atgctatcag tgaagccacg caattcgacg ttttagtaaa
     3241 cggtaagaaa gtcgacagct ataccgcaaa tatcaaaaat gtgaaaaaga ctttgggtaa
     3301 caaattctac gacagctgct ggaacacctg gttccaggaa taattgataa cgtggtgtaa
     3361 aacacctcag tttaatttaa tacaatgagt gaaaatgaat attataaaaa tattttttgt
     3421 ttcagatgtc atatatttta ttaactgaat gattaacagg agaatcagat gaactggcat
     3481 tatgaaaaaa atggtgtacg ttatgataac gtaacagagg aagatattat cgaacgtatc
     3541 cggcgtggtg aactgaatgc atcaacactg gtgtggcaac agggcatgct ggaatggcag
     3601 ccagtttcag ccacatcact ggcggaaata ctgaaacaaa gtgaagttcc cccagccctt
     3661 ccgggtcacc gcattcccgg aggcgttgtc tggacgctgg catttgcccc tttgattggg
     3721 tacatacttg agatgtggac tgcagggctg aacggtatgt catttgaacg ggcttatgat
     3781 gcagtcagcg aaggccagta ctggtttatc tctctgattc tgaatattgc tctgggatat
     3841 ctggatgaac gaaaactccg taaatctggt gttgatactg caacatttgg ctggctggca
     3901 tggttgatcc cggtttatct gtggcgtcgg gcaaaaatcc tcggacaaaa acctgcgtat
     3961 ttctgggtct ggttggttat gctgatactg gtgttattta cggtctgata ttctttatcg
     4021 cagagtttac taaataatca ggttattagt gttcttaaca g
//
LOCUS       DAZPLI010000249         4044 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZPLI010000249 DAZPLI010000000
VERSION     DAZPLI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.252"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            29..757
                     /gene="mpaA"
                     /locus_tag="ACHUU3_004135"
     CDS             29..757
                     /gene="mpaA"
                     /locus_tag="ACHUU3_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR2084054.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(732..1697)
                     /gene="ycjG"
                     /locus_tag="ACHUU3_004136"
     CDS             complement(732..1697)
                     /gene="ycjG"
                     /locus_tag="ACHUU3_004136"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR2084055.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1816..2322
                     /gene="tpx"
                     /locus_tag="ACHUU3_004137"
     CDS             1816..2322
                     /gene="tpx"
                     /locus_tag="ACHUU3_004137"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR2084056.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2366..3907)
                     /gene="tyrR"
                     /locus_tag="ACHUU3_004138"
     CDS             complement(2366..3907)
                     /gene="tyrR"
                     /locus_tag="ACHUU3_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR2084057.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          969 a         1140 c          971 g          964 t
ORIGIN      
        1 tgtaatactt cctgaaccag agacgatcat gaccgtaacc cgcccacgcg ccgaacgcgg
       61 cgcatttccg cccggaacag aacattacgg acgttcatta ttgggcgcgc cgttaatctg
      121 gtttcctgcc cctgcagcca gccgcgaaag tggtttgatt ctggctggca ctcacggtga
      181 tgaaaactct tcggtcgtca cgctctcctg tgctctgcgg acgttgacac cttctttgcg
      241 ccgccatcat gtggtgctgt gtgtgaatcc tgacggctgc cagctggggt tacgggccaa
      301 tgctaatggt gtggatttaa acagaaactt tccggcggcg aactggaagg aaggtgaaac
      361 ggtttatcgc tggaacagcg ccgctgaaga acgtgatgtc gtattactga caggtgacaa
      421 acctggctca gaacctgaaa cccaggcgct gtgccagctt atacaccgca ttcaacctgc
      481 ctgggtggtc tcattccacg atccactggc ctgtattgaa gatcccagac acagcgaatt
      541 aggcgaatgg ctggcccagg cgtttgaatt gcctctggta accagcgtcg gttatgaaac
      601 gcctggctct ttcggcagtt ggtgtgcaga tctcaacttg cattgcatca ccgcagagtt
      661 tccgcccatc tcctccgacg aagccagcga aaaatatctc tttgcgatgg caaacttgct
      721 gcgctggcat cctaaagatg caattcgccc gtcgtgaact gaagcgccgg ttccacatct
      781 accgccagcc aggtcggtcc gtcaagatcg gcgaaactga cctgcggcac cagcggtaaa
      841 gcggcgctaa tggcacgaga ggtacacaac atgcagccca gcatcagact gaaaccttgt
      901 gcacgcgctt cagtcgccag cgccagcgct tccgtcagac caccgatttt atcgagctta
      961 atgttaacca tctcatagcg ccctttcagc gccttcagat tgctacgtgt atgacaactc
     1021 tcatcagcac aaatcggcaa cggatgaata aaattctcca gcgccgcatc gtcctgcgca
     1081 ggaaggggtt gttcaagcat cgcaacgcct aaatccgcca atagctggca acgcgccgcc
     1141 aacccttctg cacgccagga ttcatttgca tcaacgatca gcgtcgcatc gggcacagct
     1201 gtgcgaattg ccaccatccg ctcactaata agatggttat ccagcttcac tttcagtaat
     1261 ttcgcgcctg cctgccagag tgttgatgca ctattggcca tctgatcagg cgtaccgatg
     1321 actaccgtct gtgcagtgat aactgtctcg ggaagcgtta tgccgatcaa atcagccagc
     1381 gattgctgct gttttcgcgc ggccagatcc cacaatgcac aatccagcgc attacgtgct
     1441 gcgccggcag ggagaatttt ttgcaactcc tcccgtgtca gccctttctc taattgcgac
     1501 acgacgctca taatttgcgc cattaccgag gcatcacttt ctccataacg cggatacggc
     1561 gtgcattcgc cggtgccttt aataccctct tcttccagtt caaccaccac cacgcgcgct
     1621 tcactgcgac ttccccgggc aatcacaaac ggggtatgta agggccaggc ttcctcaaat
     1681 accttaacgg ttctcatctt tactccttgt tgtcgcggtg gtctgcggat taagcgcaaa
     1741 tagcgtttgc tgtgttattg acagttagca taaactaggt gtgacgttaa ctatatgtaa
     1801 acaggaaaga taatcatgtc acaaaccgtt catttccagg gcaacccggt tacagtcgcc
     1861 aattccatcc cgcaggcggg tagcaaagcg cagactttta ctctcgtggc aaaagatctg
     1921 tctgacgtca ccctcggtca gtttgcgggt aaacgcaaag tgctgaacat tttcccgagt
     1981 attgataccg atgtttgcgc cgcatcagta cgcaagttta accaactggc aactgagatc
     2041 gacaacaccg ttgtgctgtg tatctctgcc gatctgccgt tcgcccagtc tcgtttctgc
     2101 ggcgcagaag gtctgaacaa cgttatcacc ctctccactt tccgtaacgc tgaatttctg
     2161 caagcttacg gtgtggcaat tgctgatggc ccactgaaag gtctggcagc gcgtgccgtt
     2221 gtggttattg acgaaaatga caatgtgatt ttcagccagc tggtggatga aatcaccacc
     2281 gagccggatt acgaagcagc tctggctgta ctgaaagcat aagttaatat gcctgatggt
     2341 gttgcaccat caggcatatt cgcgcttact cttcgttctt cttctgactc agaccatatt
     2401 cccgcaactt attggcaatc gcggtatgtg aaacgccgag acgttttgcc agtttgcgcg
     2461 tgctgggata attgcgataa agctgggtta ataccgagcg ttcaaaacgg ctggtgattt
     2521 cgtccagcga accttccatc gcatcttcgc ccacggctac cgttgcggcg tcataatccg
     2581 gcaacaaaat atcctgtgga cgcagctcat aaccgtccag ttgtgtcagt gcgcgataga
     2641 tagcgttctt taactgccgc acatttcccg gccacgcata acgcgtaagt acagtattca
     2701 ggtcagcggc cagtttcgga cgctgcacgc cctgctcgtc ggcaaagcgg gcgacgaaca
     2761 gctcagttaa cggcatgatg tcctgcggac agtcacgtag cggcggcaga ttgagcgtca
     2821 acacgttcag acgataatag agatcttcac ggaacatgcc tttttgcacc agttcgacca
     2881 gattcttctg cgtaacgcaa atcacccgca catcgacatg cacctcatgg tcttcgccaa
     2941 cccgacggaa agtgccatca ttaaggaaac gcagtaattt cgcctgcatc cgtggtgaca
     3001 tttcccctat ttcatccaac agcaccgaac caccgttcgc ctgctcaaag aatcctttct
     3061 tcccttccgg agcatgacca aacagttcac tctcgaccgc atcttccggt atagacgcac
     3121 agttcagcgc caggtaaggt ttgcccgctc tggggcttgc ctgatggcag gcgtaggcaa
     3181 agagatcttt acctgtacct gtgtcacccg taatcagcag cggcgcgctt agcatcgcca
     3241 gtttctgcgc ctgttcgaca acatgcttca ttttcgggct gacggcgaca atttgactga
     3301 aggcgctgac gtcctgggcg gcgacatttt gcaactggcg gcccatacga atcgttgatc
     3361 gcaacatcac caccgcaccg gtcaggacgt gttgatcatt ttcatcctga agataaacag
     3421 gcgtaatctc catcaggaaa ttctgcccat taataacgac atgctcgtta tgcgaatctt
     3481 gcggttcgct ttccagccaa cgtaaaaaat taaagccgtt aatcaattgt gcggcggtat
     3541 ggttgcgcag gcgatccaat ttttgcccaa aaagctgaca gctcgccggg ttcgccatat
     3601 ccactttgct tttcatatcg acagagagca caggttcagg caacgcctcc agtaacgcgc
     3661 tcaacgccag atgctcacgt tcggaaggca tccacgggac agtacgcaca tcggtaacac
     3721 ccgcaatacg gcgtatttcg gccatcagac tgctgaaact ctcaaactcc agttcagcaa
     3781 aattgaggta gattcgccca atgggatcaa tctcaatacc gcgtaaatca atgcctctta
     3841 gcacgagtag atcgagtaat tcgcgggtca gaccgagtcg gtcttcacaa aagacttcca
     3901 gacgcatggg aaccttcacc tgaaaaaaga acaattaata tgatgatatg acactatttg
     3961 gatagcagga aggtttctgt caacaatcat tgacacaaag acggaaaaac gggcacttcg
     4021 gcgtaaagat atccggtcag cacc
//
LOCUS       DAZPLI010000250         4043 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZPLI010000250 DAZPLI010000000
VERSION     DAZPLI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.253"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            926..1642
                     /gene="nanC"
                     /locus_tag="ACHUU3_004139"
     CDS             926..1642
                     /gene="nanC"
                     /locus_tag="ACHUU3_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR2084058.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1662..2768
                     /gene="nanM"
                     /locus_tag="ACHUU3_004140"
     CDS             1662..2768
                     /gene="nanM"
                     /locus_tag="ACHUU3_004140"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR2084059.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2833..3813
                     /locus_tag="ACHUU3_004141"
     CDS             2833..3813
                     /locus_tag="ACHUU3_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR2084060.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3923..4043
                     /locus_tag="ACHUU3_004142"
                     /pseudo
     CDS             3923..4043
                     /locus_tag="ACHUU3_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT         1219 a          846 c          915 g         1063 t
ORIGIN      
        1 ggagataagg gggaacgcag gagacgcttt ttcatctaca ttcgtgggta aatatggctt
       61 tggtcactgc gacttaaacc ttcctcaatc acagctattc gccgggcgat acggcaaaca
      121 aactaagtga aacatcatcc tgaaaacacc aacatcaaca agcctctcta gatcgacttc
      181 agaagcgacc agttacaagc cacaaatcag gaaacatatt tcatggtgag aattatgatc
      241 agcctgatgg cgggtaaaca cttgttttca ccacgctcac tgagttagct tgcaaagctt
      301 cccttgttta gaccctaaat aaatttctta taaaacaaac agttaaaaga aatcctttag
      361 tttttgaaga ccacctcaag tgttcgtctg gcttcacatg gcatcttcct cttagaaaaa
      421 gatcgacata ttttgtgaca cgaattgcaa atctcgtttt gttgtatggc ttgcgtgatt
      481 tttgatctgg tataacaggt ataaaggtgc accgcgatag tcaatgagac agggcatctc
      541 gcaatctatg gcaaacatca cttcagttct ttctcatcgg gtgatgaaaa cgcacttcag
      601 tctgaaagga atatgaaaat gagatcaaca gacactctat tttatgactc tgggtaaaat
      661 ggattgagta agtgatatag cttacgaaca ttcaaatcaa ttaaacatca gaagagattt
      721 tatactcagg tatttaatct gattctctgt ttatttaaat aatgtggaaa gagaattttc
      781 acaggagacc ttatacaaaa atataaaata agctacccgt tgccaaagac actataagcc
      841 tggcaaaaaa tattacacaa cataaatgct aattgtttat gcgggctttg tattgctttc
      901 tgtatcctac aaatgagtga aatttatgaa aaaggctaaa atactttctg gcgtattatt
      961 actgtgcttt tcgtcaccat taatttctca ggctgcgaca ctggacgtac gtggtggata
     1021 tcgtagcgga agccatgcct atgagactcg actcaaagtc agtgagggat ggcaaaatgg
     1081 atggtgggca agcatggaaa gtaatacctg gaataccatt catgataata aaaaggaaaa
     1141 tgccgcactc aatgatgttc aggttgaagt taattacgcg attaaacttg atgatcaatg
     1201 gacggtgcgc ccgggaatgt taacgcattt tagcagcaac ggcacacgct acggacccta
     1261 cgtaaaactg tcctgggacg cgacaaaaga tcttaatttt ggcattcgct atcgttacga
     1321 ctggaaagct taccgacaac aagacttatc cggtgatatg tctcgtgata acgttcatcg
     1381 ttgggatgga tatgtcactt accatattaa tagtgatttc accttcgcat ggcaaacgac
     1441 gctatacagc aaacagaatg attatcgcta tgcaaaccac aagaaatggg cgacggaaaa
     1501 tgcctttgtt ctacaatacc atatgacgcc cgatattacg ccatacatag aatatgacta
     1561 ccttgaccgc cagggtgttt acaacggcag agataattta tcggaaaaca gttatcgcat
     1621 tggtgtgtca tttaaactgt agtagacagg agacagtcac aatgaataaa actataatgg
     1681 cgcttgctat catgatggct tcatttgccg caaacgcgtc tgtattaccg gaaactcctg
     1741 tgccatttaa aagtggtacc ggagcaattg ataacgacac tgtctacatt ggtttaggta
     1801 gcgcaggtac agcatggtac aagctagata cacaggccaa agataaaaaa tggactgcgt
     1861 tagctgcatt ccctggtgga ccgagagatc aagcaacctc tgcatttatt gatggcaatc
     1921 tatatgtgtt tggcggcatt ggcaaaaaca gcgagggatt gactcaggta tttaatgacg
     1981 tacacaaata caaccccaaa accaatagct gggttaaatt gatgtcgctc gcgccgatgg
     2041 gcatggcggg tcatgtgact tttgtacaca acggcaaggc ttatgttact ggcggtgtta
     2101 accagaatat cttcaatggc tattttgaag atctcaacga ggctggaaaa gattcaaccg
     2161 ctatagataa aatcaacgcc cactattttg acaaaaaagc agaagattat ttcttcaata
     2221 agtttctgtt gtcttttgat ccctcaacac agcaatggag ttacgctggc gaatcgccct
     2281 ggtacggaac ggctggtgcg gcggttgtga ataaaggtga taaaacctgg cttattaatg
     2341 gcgaagccaa accaggattg cgaacggatg ccgtatttga acttgatttc accggtaata
     2401 atttaaaatg gaataagctt gatcccgtct catcaccaga tggcgtcgct ggcggttttg
     2461 cggggataag caatgattct cttatatttg ccggaggggc cggattcaaa ggttcacgag
     2521 aaaattacca gaacggtaag aactatgcgc atgaaggcct gaaaaaatca tatagcactg
     2581 atattcatct ttggcataac gggaaatggg ataaatcggg tgaattatcg caaggtcggg
     2641 cctacggagt atcattgccc tggaataata gtttattaat tattggcggt gaaactgcag
     2701 gcggcaaagc ggtgacggat tcagttttaa tttctgtgaa ggataataaa gtcacagtac
     2761 aaaactaacg cttcagggcc ccggtaaagg ggccctgcta tcaatttgtc atttgtatta
     2821 cgcgaaaatt atatgaacgc aataatatcg cccgattatt actatgttct taccgttgct
     2881 ggtcagtcta atgccatggc gtatggcgaa ggactgccat taccggacag ggaagatgcg
     2941 cctcattcca gaattaaaca attagcgaga tttgcgcata cgcatcccgg aggcccgcca
     3001 tgtcacttta acgacattat tccactgact cactgcccac acgatgttca ggatatgcag
     3061 ggttatcacc atcctctggc aacgaatcat caaacacagt acggcaccgt tggccaggca
     3121 ctgcatattg cacggaaatt actgcccttc attcctaata atgcagggat tcttatcgtt
     3181 ccgtgttgcc gtggcggatc ggcttttacc gcgggcagcg aagggacata ttcagaacgg
     3241 cacggagcca gccatgatgc ttgtcgttgg ggaacggata ctccgctgta ccaggattta
     3301 gtcagcagaa cgcgagccgc actggcaaaa aatccgcaga acaaattcct cggcgtatgc
     3361 tggatgcaag gcgaatttga cttaatgacc agtgactacg cgtcacaccc tcaacacttt
     3421 aatcatatgg ttgaagcctt tcgtagggat ctaaaacaat accattctca gcttaataat
     3481 attactgacg cgccatggtt ttgcggcgat accacctggt actggaaaga aaatttcccg
     3541 cattcgtatg aagctattta tggcaattat caaaataatg ttttagccaa tattattttc
     3601 gtcgacttcc agcaacaagg tgaaagagga ctgacgaacg cgcctgatga agacccggac
     3661 gatttaagca cgggatatta cggttcagcg taccggtcac cggagaactg gacgacggca
     3721 ctgcgaagca gtcatttcag cacggcagcc cgtcggggga ttatttctga cagatttgta
     3781 gaagcaattt tgcagttttg gcgcgaaagg tgagcgggga gtttattcat gggggatgtc
     3841 cttgttcgtt ctcagagtag acaacaaaaa acaaataaga tcaaggtaat atctcttcac
     3901 ttgcgggctc gaacagcagg caattgccaa tataataatc tggcacaagg gtgaaaaacg
     3961 cgcaccgcat aaaccgttgt tattgcttac agattagccg gatgccttaa tagacatccg
     4021 cgccttttcg attatggctc gga
//
LOCUS       DAZPLI010000251         4022 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZPLI010000251 DAZPLI010000000
VERSION     DAZPLI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.254"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..366)
                     /locus_tag="ACHUU3_004143"
     CDS             complement(<1..366)
                     /locus_tag="ACHUU3_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR2084061.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSE"
     gene            complement(381..1127)
                     /locus_tag="ACHUU3_004144"
     CDS             complement(381..1127)
                     /locus_tag="ACHUU3_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR2084062.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLADAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            complement(1134..1442)
                     /locus_tag="ACHUU3_004145"
                     /pseudo
     CDS             complement(1134..1442)
                     /locus_tag="ACHUU3_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
     gene            complement(1821..2333)
                     /locus_tag="ACHUU3_004146"
                     /pseudo
     CDS             complement(1821..2333)
                     /locus_tag="ACHUU3_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
     gene            complement(2346..2768)
                     /locus_tag="ACHUU3_004147"
     CDS             complement(2346..2768)
                     /locus_tag="ACHUU3_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR2084063.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVIVH"
     gene            complement(2765..3007)
                     /locus_tag="ACHUU3_004148"
     CDS             complement(2765..3007)
                     /locus_tag="ACHUU3_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR2084064.1"
                     /translation="MNKISTYRKQLGLSQRQLAVQLGWIQSRLANYEANFRTPGLEEC
                     RKIVSTLNRLGAHCGLDDVFPPDGKHSENSIGAVDS"
     gene            3104..3523
                     /locus_tag="ACHUU3_004149"
     CDS             3104..3523
                     /locus_tag="ACHUU3_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR2084065.1"
                     /translation="MEKENEKIAASRLNDKIAMRLKERRQKLGLSQGKLAEICGWTQS
                     RIGNYEAGSRNVGVHDAVVLGKALGISPPELLFGEQESSELWLNESQRKLLELFNQLP
                     GSEQQRMIELFEVRLKEIDEYVEKYLRGRIKDNPPPE"
     gene            3829..3984
                     /locus_tag="ACHUU3_004150"
     CDS             3829..3984
                     /locus_tag="ACHUU3_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR2084066.1"
                     /translation="MNTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT         1008 a         1080 c          893 g         1041 t
ORIGIN      
        1 ttcgctcttc cggcactcgc gacagattat attcaggcgc ctgtcgtagc ggcgtatttc
       61 tccgtctggt aatgaccaga taaggtcagg atcaaccaca accggtttct tcacctttac
      121 cctcgatagc tttttgcggg cgttttgcca gtctttatga gcctgttcag agggaaatac
      181 cccgtagcca gaattatata catcaccact tgcgaccagt tccatgcata agcgaccgac
      241 agacgcacga ctgatacctg tttcatctga caactgccga atcgtgaccc gaccgtcctg
      301 atgcacgagt tccacaattc gcgtcttcag ttcttcccgc tgttcgggag taaaaggttt
      361 cgccataaat cctcctgaaa ctacttaaca accctcgaat ggctaacatt cggacgccag
      421 ctctcccagt taaaattcac ccagcgcccg ccgttcatga ccatgcgatc catcacccgc
      481 tcgccgagaa gtgtattcat cgctgcatgg ttaatatttg tcagcatccc cacaccgcgt
      541 aacgatgccg tccggcgatc aacaatctgg ttcagtacca cctgctcgtt tttcgtatcc
      601 cgctgcatgc caatttcatc caggaccagc aggtcaacac cacaaagctc ctgtaaaaat
      661 ttttcaccgg atttgccgtt gtcgtagctc tcatgcaaca cgctcatgac atcggacacg
      721 gtgacgataa tcacgctacg ccccttcgcc atcagccggt tgccaatcgc gtctgcaaga
      781 tgattttttc cggtgccggg cttaccgctg aacacaaaat tcgtacagcc tgtcatcagt
      841 tcgtcagcta tggatttcgc ctgactcagt gcatgtcgct gaccgtcgtt ctgcgcccgg
      901 taattcgcaa acgaacactt ccggtgcaac ggctggatgc cggagcggtt caggattttt
      961 tccacccgca actgacgatt caggcggttg atctcctcgc aacgtttctg gccttcagca
     1021 agttgccact cgcgccactc cgcaaccgtt ctgaatgggg cggttacatg tggtggggtc
     1081 agtctgcgga tacgctccag aacgcctcct gtcgcaatat ttttcatggt ctgttacccc
     1141 ctgaagcctg gcgggatcgc actgtccggc aacgagacgg tgttaacctg tcggagcaac
     1201 gtctcaggcc gaacaccttt cggcgcgaac agaccctggt attcattggc gatgctgtgt
     1261 cgaatcacct gctcaggtgt aaaaccctgc tgacggaatt tttccagttc ccgtatcgcc
     1321 ccgttagcgc cctgctccgt tcgaatcggt tttcgcaatg cctgcctgaa ctggacccac
     1381 tcatgccaga gtgtttctgg caaccagtcg ggcagatcaa ctgacaacgg gtcaaatttt
     1441 ttctggcgaa cagccccacc gataaccaga attccctgct cggtgtgcct tgcgatatca
     1501 ctgcgccgca agacacatat cccggtttca acagcaccga ttttgagcag tgatttctgt
     1561 cgcggagaat ttcggtcaag ctcgctccca caccggacac cgttcctgtc agtcacgacg
     1621 atatcaatcc gccctccgca accgtcacca cgctctggca ccggatactc acgacagaca
     1681 tccaggccag cagcctgcaa cgcaaccacc gcagaattac aaaattccgc tgcagtaccg
     1741 ccagataatc tcccctcaag cactccagcc acaaaatccc gaaactccct tccggaaggg
     1801 aagggtttgg gatgggttag atctgttttt agatctttat ctgtatcttt attagttgcc
     1861 tttgtgttgg catcatgttc gaacatcact ccaacatctg tttgaacacc tgttaaattt
     1921 ctctcttgtt ttgtttgaac atctgcttcc tttctgcttc ttctggcctg aacagatgct
     1981 tttcctgcgg ctgatttttt ggttaatttt tctctgacag atgccagatc ttcctcaatc
     2041 cgaagatgca tccattcgtc gccgttatca caaaaaaact cccgcaagga tggttcaaca
     2101 tcaacccatc gctcgttagt cagacgggca atttttgcca acctgttttt gggtattggc
     2161 tttcctgttt gccagtaatt gaacatcagc aacaaatacg cgccgtgctc ctctgctgac
     2221 aaatgcatgg tgtcagccag gtaatcagct atgtacagtt gcatgtatgg taatgcggcc
     2281 ataattgccc cgtatgatgc tgcccggttg cttagaataa gcacaaacag catggaaact
     2341 tttgcttaat gaacaatgac agaatcgtcg gaagacccgc cgccgctgaa atgcgctttc
     2401 cggtaaacgg cctggactgc atcatcatgc gcatcaattg ccgtactcaa cgcttcctgc
     2461 gccgccagta atgcacggcg ttccagggta tcgaagatgc agagtcggtg acgcagctcg
     2521 cgcggaagaa ttgccagaac cgcagggatc agtttctgaa ttttttccct ttgcgctttc
     2581 gtttcacctt tcaaccaacg gtgatagata ttctgctgat tgttccagtc cttgcctggt
     2641 acaaggggca attcgccgcc cccctggcgc agatattctt cagtaattgc gttagcgacc
     2701 cacgcctgcc ctttttcggc tgccagggct aacaacactg attcgatgtg ctcatgcctg
     2761 attttcatga atcaaccgct cctatgctgt tttcgctatg cttaccgtct ggggggaata
     2821 catcgtcaag tccacaatga gcgccaagcc gattaagggt agaaacaatt tttctgcact
     2881 cctctagtcc aggggtacga aaatttgctt cgtaatttgc cagtcggctt tgtatccacc
     2941 ctaactgaac agcaagttgt ctttgagaca gcccaagctg ttttcgatat gttgaaattt
     3001 tgttcattga aaacctccga tgacaatttt aaacacacct tgtgttatat agccaagctg
     3061 ttttgtgttt tatgtaaatc acgattcgtg atacaaggat gcaatggaaa aagaaaacga
     3121 aaaaattgcc gctagtaggc tcaatgacaa aattgcaatg cgtcttaaag agcgcaggca
     3181 gaagcttggt ttatctcaag gaaaacttgc tgaaatctgc ggatggacgc aatcgcgtat
     3241 aggtaactat gaggcgggca gcagaaatgt tggagtacat gacgctgtcg tattgggaaa
     3301 ggcacttggc atatctcctc ctgagctcct ctttggagaa caggaatctt ctgaattgtg
     3361 gttaaatgaa tcccaacgaa aacttcttga gttgtttaac cagctaccgg gctcagaaca
     3421 acaacgaatg attgagctat ttgaagtccg gctaaaagaa atcgatgagt atgtagaaaa
     3481 atatttgaga ggcaggatta aagataatcc cccaccggaa taatgatctt gctatcacag
     3541 taatatgcca atcagcccgc tatcagcggg cttttttgta gcatcatcat atgacactca
     3601 ccataaaaca cattgcgtgt taacacaaga aaacaaattg tgtttaataa gcatatccaa
     3661 acaacgcccc accagagaac ggcaggacaa tacctcgagt tatccagtca ctgaacaggg
     3721 ctaagtagcc agcctgaggc atacgaacat gacggcagtt gttgattgat acaaagcgca
     3781 gtagataaaa cgttccgcca cccggcgtta aggggaaatg gggtcaacat gaatactatc
     3841 gatcttggca acaacgaatc tctggtatgt ggcgtgtttc ccaatcaaga cggcacattc
     3901 accgccatga cgtataccaa aagcaaaacg tttaaaaccg aagctggcgc acgtcgttgg
     3961 ttgggaagac attcaggtga gtaaaatgaa cgagacagaa ttaaaacacg ttatcgctct
     4021 ac
//
LOCUS       DAZPLI010000252         3959 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZPLI010000252 DAZPLI010000000
VERSION     DAZPLI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.255"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..242)
                     /locus_tag="ACHUU3_004151"
     CDS             complement(<1..242)
                     /locus_tag="ACHUU3_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR2084067.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACHUU3_004152"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACHUU3_004152"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR2084068.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="ACHUU3_004153"
     CDS             2255..3904
                     /locus_tag="ACHUU3_004153"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR2084069.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1031 a         1001 c          927 g         1000 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtaacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccacc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 tagacgccga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaagatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gaagcgcggt ataacgcgtt ggcggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaaacctaa aaccttatta ttttcactga cagcggatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagtgagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc ccggacagta aaacctttcc cgactgcgca cctaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctac tggcgctgcc gcagtcatat attgtcccag gcggccgttt
     2761 tagcgaaacc tactattggg actcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttactggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggggcggcc tccggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagg
//
LOCUS       DAZPLI010000253         3946 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZPLI010000253 DAZPLI010000000
VERSION     DAZPLI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.256"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            30..968
                     /locus_tag="ACHUU3_004154"
     CDS             30..968
                     /locus_tag="ACHUU3_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFR2084070.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDNPPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            1024..2313
                     /locus_tag="ACHUU3_004155"
                     /pseudo
     CDS             1024..2313
                     /locus_tag="ACHUU3_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            2310..2603
                     /locus_tag="ACHUU3_004156"
     CDS             2310..2603
                     /locus_tag="ACHUU3_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR2084071.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            2768..3946
                     /gene="fadK"
                     /locus_tag="ACHUU3_004157"
                     /pseudo
     CDS             2768..3946
                     /gene="fadK"
                     /locus_tag="ACHUU3_004157"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
BASE COUNT          929 a         1003 c         1088 g          926 t
ORIGIN      
        1 cctgaactga agcctacgga gaagaaacga tgagtcaatt aaacagcgtc tgggtcttta
       61 gcgataatcc tgaacgttat gctgaactgt ttggcggcgc gcagcaatgg ggccaacagg
      121 tgtatgccat tgtacaaaat accgaccagg cgcaggcagt tatgccttat ggtccaaaat
      181 gtatttatgt tcttgagcaa aatgatgcgc tgcaacgtac tgaaaattac gccgaaagca
      241 ttgctgccct gctgaaagat aacccccccg ctatgttgct gctggccgcg acgaaacgtg
      301 gtaaagcgct ggcagcacgg ttaagtgtgc aactgaatgc ggcgctggtg aacgatgcca
      361 cggcagtgga tattgtcgat ggtcacattt gcgccgaaca ccggatgtat ggcgggttag
      421 cgttcgctca ggaaaagatc aacagcccgc tggcgatcat tacccttgca cccggtgttc
      481 aggaaccgtg caccagtgat acctctcatc agtgcccgac agaaacggta ccttatgttg
      541 ccccgcgtca tgaaattctc tgtcgcgaac gccgtgcgaa agcggcaagc agcgtggacc
      601 tgagcaaagc aaaacgtgtg gttggcgtcg gtcgtggtct ggcagcgcag gatgacctga
      661 aaatggtcca cgaactggcg gcggtgctga atgctgaagt cggctgttca cgtccaattg
      721 ccgaaggcga gaactggatg gagcgtgaac gttatatcgg tgtctccggc gtgttgctga
      781 aatccgatct ctacctgacg ctggggatct ccgggcagat ccagcatatg gttggcggca
      841 acggcgcaaa agtgattgtc gccatcaata aagataaaaa tgcgccaatc ttcaattatg
      901 ccgactacgg tctggtgggc gatatctaca aagtcgtccc tgccctgatt agccagttga
      961 gccgctaatt cccctttcaa ccacatctcc cgctgtaaac gcagcgggaa gggagcataa
     1021 cgcatgtcgg atgacaaatt tgatgccatt gtggtcggtg cgggcgttgc tggtagcgtt
     1081 gccgcactgg tcatggcgcg agccgggctg gatgtcctgg tgatagaacg cggcgacagt
     1141 gccggatgta aaaacatgac cggcgggcgt ctttatgccc acacacttga agcaatcatt
     1201 ccaggctttg cagtatcagc gccggtagaa cgcaaggtca cacgcgagaa aatctccttc
     1261 ttaaccgaag aaagcgccgt taccctcgat tttcaccgcg agcagccaga tgttccgcaa
     1321 cacgcatctt ataccgtatt gcgtaatcgt ctggacccgt ggttgatgga acaagccgag
     1381 caggcgggcg cgcagtttat cccaggcgtt cgcgtcgatg cgctggttcg tgaaggaaac
     1441 aaggtaactg gcgtgcaggc tggggatgat attctcgaag cgaatgtggt gattctggct
     1501 gatggcgtta actcgatgct tggccgctcg ctgggaatgg ttcccgcttc cgatccgcat
     1561 cattacgctg ttggtgttaa agaggttatt ggcctcacac cagaacagat caacgatcgc
     1621 tttaatatta cgggcgagga aggtgccgcc tagctgtttg ccggttcccc ttctgacggc
     1681 ctgatgggtg gcggattcct ctataccaac aacgattccg tatcgttggg gctggtttgt
     1741 ggattgggtg atatcgccca tgcgcaaaaa agcgtgccgc aaatgctgga agattttaaa
     1801 caacaccccg ccattcgccc actaattagc ggcggcaaac tgcttgaata ttccgcgcat
     1861 atggtgccgg aaggcggtct ggcgatggtg ccgcaactgg ttaacgaggg cgtgatgatc
     1921 gttggtgacg ccgcaggctt ctgtctgaat ttgggtttta cggtccgcgg catggattta
     1981 gccattgcat cggctcaggc tgccgccaca acggtgatcg ccgccaaaga acgcgaggat
     2041 ttctccgcca gcagtctggc gcaatacaag cgtgagctgg aacaaagctg cgtcatgcgt
     2101 gatatgcagc attttcgcaa gatcccggcg ctgatggaaa acccgcgcct gtttagccaa
     2161 tacccacgaa tggtagccga catcatgaac gagatgttca ccatcgacgg caaaccaaac
     2221 cagccggtac gaaaaatgat catgggacat gcgaagaaaa ttgggctgat caacttgctg
     2281 aaagatggca ttaagggagc aaccgcgcta tgagccagaa cgctacggtt aacgtcgaca
     2341 tcaaattagg cgtcaataaa ttccatgttg atgagggcca cccgcatatc attctggcgg
     2401 caaatcccga catcaatgaa ttccgtaaat taatgaaagc ctgccctgcc ggactttata
     2461 agcaggatga cgccggcaac attcattttg attctgccgg ttgtctggag tgcggcacct
     2521 gtcgggtgct gtgcggtaac actattctcg aacagtggca atatcccgca ggcaccttcg
     2581 gtattgattt tcgctacggc taatcatgca tcccacaggc ccgcatctcg ggcctgatgt
     2641 tctgtttcga gagtccaaca tgaaagtgac attaacgttc aacgaacaac gtcgtgcggc
     2701 gtatcgtcag caagggctat ggggcgatgc ttcgctggcc gattactggc agcagaccgc
     2761 tcgtgcgatg ccagacaaaa ttgccgtggt cgataatcat ggtgcatcgt acacctatag
     2821 cgcgctcgat cacgccgcga gctgtctggc aaactggatg ttagcgaagg gtattgaatc
     2881 aggcgatcgc atcgcatttc aactgccagg ctggtgtgaa tttaccgtta tctatcttgc
     2941 ctgcctgaaa accggtgcgg tttccgtgcc gctgttgcct tcctggcggg aagcagaact
     3001 ggtgtgggtg ctcaataagt gtcaggcaaa aatattcttt gcaccgacgt tgtttaaaca
     3061 aacgcgtccg gtagatttaa tcctgccgct gcaaaatcag cttccacaac tacaacaaat
     3121 tgtcggcgtg gacaaactgg ctcccgccac ctcttccctc tcattaagtc agattatcgc
     3181 cgacaatatc ccactgacca cggcgataac gacccacggc gatgaattag ctgcggtgct
     3241 gtttacctcc ggaaccgagg gtctgccaaa gggcgtaatg ctaacgcata acaatattct
     3301 cgccagtgag cgggcttatt gcgcgcggct gaatctgacc tggcaggatg tctttatgat
     3361 gcctgcgcca cttggtcacg caacgggatt tctgcatggc gtaacagcac catttttaat
     3421 tggtgctcgc agcgtgttgt tagatatttt cactcctgat gcatgtctcg cgctgcttga
     3481 gcagcagcgt tgcacctgta tgctcggcgc aacgccgttt gtctatgatc ttttgaattt
     3541 actagagaaa caacccgcag acctttcagc gctgcgcttc tttctttgcg gcggtaccac
     3601 aatccccaaa aaagtggcgc gcgaatgcca gcagcgctgg cattaaatta ttaagtgttt
     3661 atggttccac agaaagttcg ccgcatgcgg tggtgaatct cgatgatcct ttgtcgcgct
     3721 ttatgcacac cgatggttac gctgccgcag gtgtagagat taaagtggtc gatgacgcac
     3781 gcaagacctt accgccaggt tgcgaaggtg aagaagcctc gcgtggcccc aatgtgttta
     3841 tggggtattt tgatgaacct gaattaaccg cccgtgcgct ggatgaagaa ggctggtatt
     3901 acagcggcga tctctgccgt atggatgagg ctggctatat aaaaat
//
LOCUS       DAZPLI010000254         3946 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZPLI010000254 DAZPLI010000000
VERSION     DAZPLI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.257"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..1364
                     /locus_tag="ACHUU3_004158"
     CDS             <1..1364
                     /locus_tag="ACHUU3_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFR2084072.1"
                     /translation="SLFSASSGINWRDNLYDASFRGVPFSVESDEGSFGRRVQVHEYP
                     NRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLIHPQFGEMQGC
                     IDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVFLDELIEDMFG
                     DFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDLSVILMPPSVA
                     SDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPTDSASVIRQKQ
                     QTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQSDIIHVSHPAL
                     DSVAASTGQDERAQPPTRENLTIIRESLNAAIEQELRRTADDRLFFQLTSLRTELNRD
                     IQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHPGFVPVRALRV
                     PVR"
     gene            1361..2437
                     /locus_tag="ACHUU3_004159"
     CDS             1361..2437
                     /locus_tag="ACHUU3_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFR2084073.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLGENILSCDTERSIRERFSEYQVSGQRPGNDDD
                     FGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRETTYT
                     VQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGPADAY
                     LPEPVTARKKKMLRRISDERVFSS"
     gene            2415..2948
                     /locus_tag="ACHUU3_004160"
     CDS             2415..2948
                     /locus_tag="ACHUU3_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR2084074.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            2954..3364
                     /locus_tag="ACHUU3_004161"
     CDS             2954..3364
                     /locus_tag="ACHUU3_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFR2084075.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            3357..>3946
                     /locus_tag="ACHUU3_004162"
     CDS             3357..>3946
                     /locus_tag="ACHUU3_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR2084076.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
BASE COUNT          884 a          969 c         1182 g          911 t
ORIGIN      
        1 cttcactctt ttctgcatct tcaggtatca attggcgcga taatctttac gacgcttcat
       61 ttcgtggcgt tccgttttcg gtggagagcg acgagggttc gttcggacgc cgcgttcagg
      121 tccatgaata cccaaaccgt gacaaaccgt acacggaaga tctcgggcgt gccacgcgac
      181 ggctgacgat taatgcgtat ctcgttggtg atgattacgc agagcagcgc gacaggctga
      241 ttacagcgat tgaaaccgcc gggccgggga cgctgatcca tccgcagttc ggtgaaatgc
      301 agggctgtat tgacgggcag gtgaccgttt ctcactccgg cactgaaggc cgtatgtgcc
      361 gggtttcatt tcagtttgtt gagagcgggg aactgtcatt tccggtcgcc ggagctgcaa
      421 ccgccagaaa actggaggag tcgtccgtat tcctggatga gttgattgaa gacatgttcg
      481 gcgattttga tctcgcggga ataccggact ttattcagaa cgatgttatt gcccggacga
      541 ccgatatgct gggaaccgtt cagacagctt tcaaaatggt taattcggct gtttctgccg
      601 gagcgagact actgcagggc gatttatccg tcattctgat gccgccgtcg gttgccagtg
      661 attttgtgca tacgttgcag gatacctggc gggccggaac ccggctggtg gataacacac
      721 aggatctggt gcagtccata acgacaatgt ccggcattac gctggaccca ggactggcac
      781 cgcgtgcggt gtggcccaca gattccgcat cggttatcag gcaaaaacag cagacaaacc
      841 ttgtggctgc tgtcatccgg acgacggcaa tcagcgaagc cacaagggcg gtcacttcac
      901 tgccacaacc cggaagcctg gtgaaaaatc agcaggcggt tgtggctgtt ggtggttcca
      961 ctgaacgtca gtccgatatt attcacgttt ctcatccggc acttgacagc gtggcagcca
     1021 gcacaggaca ggatgagaga gcgcaaccac ccacgcggga aaatctcacc atcatacgcg
     1081 aatcgctgaa cgcggcgatt gaacaggagc tcaggcgtac ggcggatgac aggctgtttt
     1141 ttcagctgac gtcattacgt acagaactga accgggatat tcaggcgcgt ctggttcaga
     1201 cggaggaaac cgcagagcga acgccagcgg aagttctgcc tgcgcttgtt ctggctgcgt
     1261 catggtacga cgatgcgtcc cgtgaaactg atatcctgga tcgaaatgcc atctctcatc
     1321 cgggctttgt tccggtcagg gcattaaggg tacccgtcag atgaatgata ccgttttact
     1381 tcgggtttcc ggtcgcgaat ggggcggctg gacatccgtc cgtatcagtg cgggcattaa
     1441 ccgtattgcc cgggatttta atgttgccat taccacccgc tggcccggaa gccgggatta
     1501 tcaaccccgg ataaaaaatg gtgagctggt tgaagtgctt atcggggatg agcctgtgct
     1561 caccggatat gtggaggcac taccgcttcg ttatgacgcc agcagcgtca gcatgggaat
     1621 tgtcgggcga agcaaaacag ccgatctggt tgactgctct gctttgccac tccagcagag
     1681 cggaaaaaac ctgctcagaa tagtcagtga actggctgcg ccatttggca tcaccgttgt
     1741 tgatgctggc gtgccgcaga cagcggtgat tgatgcccag ccggaacacg gcgaaaccgt
     1801 tgccgattgt cttaaccggt tactggggca ggttcagacg ctggcttatg acgatgaatg
     1861 cgggcgactg gttctgggaa aacccggaac aggtaaagcg gcgacggcgc tggtgctggg
     1921 agagaatatt ctttcctgtg acacggaaag aagcatcaga gagcggtttt ctgaatatca
     1981 ggtcagtggg cagcgcccgg gcaacgacga tgattttggt gaggccacca ttgccgcaat
     2041 acgtcagacc attcaggaca gtggcgtgac ccgttatcgc cctttgttga ttcagcagtc
     2101 aggcacagca acgacagcaa cctgtaaggc ccgttgtgaa tttgaagcgc gccaacgggc
     2161 tgcgcttacc cgtgagacaa catatacggt tcagggctgg cggcagggca gtggcgcgtt
     2221 atggcgtccg gggttatctg tcatcgtttt cgacccgctg aataattttg ataatgatga
     2281 actggtgatc gcagaagtta cctataacca ggacgaccgg ggcacgacga ctgaattacg
     2341 ggttggcccg gcagatgctt atctccccga gcctgttacc gccaggaaga aaaaaatgtt
     2401 gaggaggatt tctgatgaac gggttttctc ttcgtaatct gatttcccgg gctgtcatca
     2461 cggcggtgga ttccgccaga aagtgtcagt ctgtagggtt gaaaatgata gccggagatc
     2521 agaaacaaca cgttgagcac cttgaacctt atggttttac atctgccgca cagaacggtg
     2581 ctgagggcgt tgctttattt ccggcgggcg atcgttctca tggtgtggtt gtggtcgtgg
     2641 ctgacagacg ttaccggctg aaaggactga aacgtgggga agtggcgctt tatgacgatc
     2701 tgggacagtc cgttgtcctg acccgttccg gtattgtggt ggacggggcc gggaaggcca
     2761 taatttttaa aaacgcgcct aaagcgcgct ttgaaatgcc agtcgaatcc accgccgata
     2821 ttactgacaa ttgcgacagt ggcggactca gcatgcagca aatgcggcag gcctacaatg
     2881 cccacaaaca taccgaaaat ggtgatggtg gcgggatcac tgacacgccg gatcaaccga
     2941 tgggctgaaa atcatgatga ttaatgttaa cgggcgaccc gtgtcgaccg gggcttcgat
     3001 cgaccttctg acgcgtgctg tgattatttc gctttttaca tggcggcgtg ccgggcgtga
     3061 tgatgcacca cggatatttg gatggtgggg ggatacctgg cctgcggttc agaatgatcg
     3121 cacggggtcg cgtttgtatc tgttgcgacg cagcaagctg acaaataaaa ccccgcagct
     3181 tgccagagat tatgtccgtg aggcgctggc gtggatggtg gaggatggtg ttgcttcccg
     3241 tcttgatatt aacgctgtcc ggaccgggac agactcgctg gcacttgcca ttaccattta
     3301 ccagcgtgac ggcaatattc acaacattat ttttgatgat atctggagtg aactgaatgg
     3361 ctgacagtca gttttatcgc cccggcctcc cgcaacttat ttctatgatc cggagcgatt
     3421 tattaacccg ctttgagcag gatacgctgc ttcgtcgtat ggatgcggaa gtgtatgccc
     3481 gtgtacaggc tgcagccgta cacacgttgt acgggtatat cgattatctt gccagaaatc
     3541 tgttaccgga cgtgtgtgat gaagactggc tgtaccggca cgccagaatc aaacgctgcc
     3601 cgcgaaaaga agcggtggca gcccggggat ttgtgcgctg ggatggcgta gaggggacgc
     3661 cggtattgcc agcgggaacg cagatccagc gtgataatca ggtgaccttt accacgacgg
     3721 cgacggtgac cgcagccgat ggtcttctcc gggtgcctgt tgtggcagac gaaccgggat
     3781 cggcggggaa tacggatgat ggtattgcca tgcaactggg aacacccgtc agtggtctgc
     3841 cgtccacagg gtacgctgac accattacag acggtgaaga tattgaaaat ctggaaatat
     3901 ggcgtgcccg cgttatggaa cgttattact acattccaca gggggg
//
LOCUS       DAZPLI010000255         3937 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZPLI010000255 DAZPLI010000000
VERSION     DAZPLI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.258"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..585
                     /gene="fimZ"
                     /locus_tag="ACHUU3_004163"
     CDS             <1..585
                     /gene="fimZ"
                     /locus_tag="ACHUU3_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFR2084077.1"
                     /translation="MSIEVLLQKNSELQIVLKTDDYRITIDYLRTRPVDLIIMDIDLP
                     GTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQ
                     MILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRYLVSGLSNKEIADKLLL
                     SNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(588..1103)
                     /gene="sfmF"
                     /locus_tag="ACHUU3_004164"
     CDS             complement(588..1103)
                     /gene="sfmF"
                     /locus_tag="ACHUU3_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFR2084078.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     KADAIFMINYN"
     gene            complement(1114..2121)
                     /gene="fimH"
                     /locus_tag="ACHUU3_004165"
     CDS             complement(1114..2121)
                     /gene="fimH"
                     /locus_tag="ACHUU3_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR2084079.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(2134..3908)
                     /gene="fimD"
                     /locus_tag="ACHUU3_004166"
                     /pseudo
     CDS             complement(2134..3908)
                     /gene="fimD"
                     /locus_tag="ACHUU3_004166"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
BASE COUNT         1094 a          972 c          861 g         1010 t
ORIGIN      
        1 atgtctattg aagttctgtt gcaaaaaaac agtgaattgc agattgtcct gaaaacggat
       61 gattaccgca taaccatcga ttatctccga acccgtcctg ttgatttaat cattatggat
      121 atagacttgc ccggaacaga cggttttacc ttcctgaaaa ggatcaaaca aatccagagc
      181 acagtgaaag tgttattttt atcatcgaaa tcagaatgct tttatgctgg cagagcgata
      241 caagctggtg ctaacggttt tgtcagtaaa tgcaatgatc agaatgatat ttttcatccc
      301 gttcagatga tcctctccgg atacacgttt tttcctagcg aaacgctcaa ctatataaaa
      361 agcaataaat gtagtacgaa tagttcaacg gtcactgtgc tatctaatcg tgaagtgacc
      421 atattacgtt atctggttag cggattatct aataaagaaa ttgccgataa gttattactt
      481 agcaataaaa cagttagtgc gcataaatct aatatttatg gcaaactagg tttgcattca
      541 attgtagagc ttatcgacta cgccaaatta tacgaattaa tataatatta attataattg
      601 atcataaata tcgcatccgc tttctccaca cctggctgaa caccgctggc taacgctcga
      661 taattggcaa aaaagtttag tgtgacattg ccatttgcat ctacttcctc agtcgggctc
      721 gcctccccca gtgcgagccg ggagcgatcg ctattacgta attcgatggc gacggtttgt
      781 gccattgcgg gatcatccag agccagcagg ttggtatcgg atgccggcgt tcccgtaaat
      841 aaaatcgcaa ctgaacccgg aggacatccc tccagccgca ggctaaaagg gacgagtgcc
      901 gtggtatcgc cagcgttcag taattgtgtc gtaggccatc tgcctaaatc taccgtctta
      961 tcaatatccg cagtgtttac ggtacaggag aaatcaacaa cgttaccgtg caaattgata
     1021 ttaatcgttc ccaaagggtc agctgcccat ccactggaac tccacagtaa cccgcagaaa
     1081 cagctaaaga gtactcttct cattctccgt acctcattga taatcgacgc gtaaataccc
     1141 cagcgcgcta aacggccctt cggtcggttt ttgaccggta atactgatag gccaggcgcg
     1201 aagtgtgaca ttggctgccg cagctgcatc cagacggaaa ggaataacgc tattgagatc
     1261 gttaggcgtg atcggcgtat cgttctgatc ggcgacaata aaacctaaat cctgattgtc
     1321 cgacaccatc gcctgaccag aaacggcact ggcttccaga cgcattgtta aataagcctg
     1381 cgcagcaaca ttcgtacatt tgatcgcaat gctcttggtt tgcggcatga cgccagctgg
     1441 tcgattcccc ggccctgccg cactaaataa cgatgcgccg atatcaccaa aatcaaattc
     1501 aacaatctgc ccggcattta attcgcagtt ttgcggcact tcaacccggc caccaaaact
     1561 aatggtataa acaggtgtaa ccaacggatc gtaaggcgtt gatgtcacat acacggtaaa
     1621 catcacctgt ctggggatct ccaccatgtt aataaaggga cgaataacct ttaaacgaaa
     1681 tatcaaacga gaatccatca ccccgaacgg aaggttttgc gaaacgttag gatctgttcc
     1741 catccgaata taattttgcg gggggaactg aatatccatc acactatcaa ccagactcat
     1801 cgcacctaat agataatcat ttaattgcat atatttataa ttatcgatag tttcctgaac
     1861 aataaagtta gtgacataac tacggtatgt ataattcacc agcgtgccgg gtggacaaat
     1921 tgctgagaca cctacccagc cggatttttc cggtaaaaca acaatctgcc ctggctggtt
     1981 attggtgcta ttaaagacat ttgagagatc atagtgttca tcttctgcta cgccagtact
     2041 atttgcacaa acagtagccc aggatatgtt tgccagacac aaacatgcca ttgccaataa
     2101 taatctgcag attatcttca ttgataattt cctttatgag ccaggatgtg tgcaaactgc
     2161 cgaaataaca gtgacggctt gctgtaagct ttgctttggc aatacatagt gcgcaatgca
     2221 gcgtgagttc gcgccgtctc cccactgaac cagtaattca ccagacaatg gcgcaccact
     2281 taaataaact tgcccgtcat cacccaccat actggttatt ccggtactgt tttcccgtac
     2341 cagtgatcca aacggcaccg gttttccgcc ctgggtaacg gtaatgagcg cccgcacgcc
     2401 tatgcgggta tcaaaattgg cacgaaccaa cgcgccttgc gtcggcacaa cgctgctaat
     2461 atttttttca acatcgatgg aattccccat cgtattggta tcaagcgcga tacggttata
     2521 ccgataaacc gtggcatacg gcatcaccgc atagccgcgc cagtcggtta aaatgccagt
     2581 ttgattttca atgcgtacac cgcctgcgcc aggcgcttta atcaaaacat tggtatcccc
     2641 taaaggctgg ctcagtgtta tgccattttc atgcccgacc acaccgccag acagctgcca
     2701 gttaacgtca tgttgatcgc ggtcgtagtt atacccgacc cccagcgtac cgtaagtggc
     2761 ctgccagttt gccgtggcgc tgcccgtgta cccattattc gaggtatcgc cctggcttac
     2821 gtgataactc aggttgtggc cttccagtaa ggtcccacca acgcctgcca gccagctatt
     2881 ttgcccgttg ctgttacggt tagcgttcaa ggaggcataa gcgcggtcga gcgcattttc
     2941 ccgggtgtag cgacgtttgg tcaaaacatt gaaaggcact gaaacattaa gtccgacaat
     3001 atgttcgtta tcggggatcc ctacagattc attccacgaa aatgagagtg aataactgat
     3061 gccaacccag ctgctggtat accccacctg ataccaggta tctgaatccg aagtattcca
     3121 gtatttttga tgggtaccag agatataaag cgagccaaag tcattaagtg attgtgaaat
     3181 atttaactgc aaacggtctt tgcggctaaa gcgtaaattg tggtaattca cgattatcgg
     3241 ttcatcgcga tgctcaccgt cgtaatcgta atcatattcg tacccctcca ttcgacgata
     3301 cgcaacatca tcaagggtat aaaaaccttg tgtcgaatag cggtaaccca ttaactgaaa
     3361 attggtgccg aaggtgtaca tcgatttcgc atagaggaag cgaatggaat ccccctcatg
     3421 acgactgtcg tcggctaact ggctgcgcgc atgggtcaca tccagcgaca ctgcgcccca
     3481 gttcccgagg ttgcgcccca gccctaataa aaaggcggtg taattggcag ataattgcgt
     3541 cccgccgtag gcagtaaatt cctgtggtaa accaccgagt gccgtgccct gaaaaaagaa
     3601 aggtgatgat tgctgactat taccgctacg aaaatcgccc gccgtcaggt caaatttgaa
     3661 acgcccttcg cgttgtaaaa tcggcactgt tgaatacgga attgtgtaat tctgccatcg
     3721 cgctcgtcga tggtgacatc cagatcgcca tttgaagatg tcgggtgcaa atcagtaatt
     3781 tcaaaagcac cgggggaaac atagctttga tagataataa aaccattttg ccgaatcgtt
     3841 agctgggccg ccgtacgggc aatcccacgt accgttgggg caaacccttg ctggctatct
     3901 ggatacatat tatcggaaga ataaagccgt acaccac
//
LOCUS       DAZPLI010000256         3927 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZPLI010000256 DAZPLI010000000
VERSION     DAZPLI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.259"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..305
                     /locus_tag="ACHUU3_004167"
     CDS             <1..305
                     /locus_tag="ACHUU3_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084080.1"
                     /translation="PRVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYS
                     NNRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            394..2262
                     /locus_tag="ACHUU3_004168"
     CDS             394..2262
                     /locus_tag="ACHUU3_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFR2084081.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            2299..3927
                     /locus_tag="ACHUU3_004169"
                     /pseudo
     CDS             2299..3927
                     /locus_tag="ACHUU3_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
BASE COUNT         1233 a          724 c          770 g         1200 t
ORIGIN      
        1 acccacgtgt agaactgtat taccgcttta accatgaaga agaatattac ccttctcacc
       61 gttttatgct tgttgttgat atgaagacag taatggtttt ttcacaacga gctgaatcac
      121 tggggttata ctcgaataat cgcttgctta tcaatataat tgccgaacat atccatagtg
      181 atatatatct aaccaagtat cagcaacaat atccagatgc caatgcatta ataaatactt
      241 tgcatgaatc tggcaatgat atggtgcagg atgaaaaaca tttgagtgat agcaaaaacc
      301 gttaatattt tcctttcatt gtgatcgtct gtaggcgatc acaatctatt tgtcttttat
      361 aacgtctaaa cttttttatg caaaggtgta tttatgattt tttttaacga tataaagccc
      421 actggttggt taaagaataa gatggaatgg tatatgaacg gtcatatagg agaactggat
      481 aaattagttc cccatcttat tacggagcat aaaatatatg gaagagatcg tataactcca
      541 cgcactgtat ctgctgaaga tggcgttatt aaaaaacaga ataatcccga tgataacatg
      601 atgcaatatt attggtggga tagtgaaact caatcgaatt ggaaggatgg atattgccgc
      661 agtgcctact tgttagataa taaagaatgg caggaaaaag cgagcgattt atgtttggaa
      721 cttatcgata cgcaagatga tgatggctac attggaattt atgattcatc attaaaattt
      781 aactgtaaag cagaaaacgg agagttctgg gcacaagcta ctgccttgcg ttttgtgctt
      841 ggatgttatg agtccacccg taatgagagt ttattaactt ctcttaccca agccgcacaa
      901 tgcattatgg ctggctaccc taaagaaacg tcacatcctt tcgttgtgga acagggtttt
      961 gctggacata gccacggatt aacaataaca gatgttttct accgccttta tgaaataacc
     1021 aataacattg aatatctctc atattgcttg tggctgtatg aagattattc cgcaggatgc
     1081 gtgtcggagc aagatctgca atataaaaat gtcatagacc ctggttacct tcttaaaggt
     1141 catggcgtac atacgtatga gcatattagg gcattagcca tcgcagcgag ccaacggccg
     1201 cagtatcaaa ctgcattaga tattttttta tcccagttga aatattatct tacaccatct
     1261 ggcgctccca ttggtgatga gtggattaca ggacgcacag ctaatgccac ttatacaggc
     1321 tatgaatatt gttctgttca tgaactattt agcacttatg ttatgttaat aaaactgacc
     1381 actaatattg gtctggccga tgatgctgag tggctattct ataatgctag ttttggcatg
     1441 cagcatccca tacttcacgc gataatgtac tgtaagactg ataattgtta tgaagcaaat
     1501 gaaaggaaac atcctgacag taatcagttt aataccagat ataaatactc ccctactcat
     1561 gaggaggctg ctgtctgctg cgtaccgaat acagcaagaa ccataccggc ttttgtcgaa
     1621 aatatgtttc aagaaaataa caatggtttt actgcattat tttatggccc gtgtgagttt
     1681 tatggcacat ttaataatgt tgcaattaaa attacacaat taactgaata ccctcatgat
     1741 ttaagcgtaa aatgtctcat tgagcctgag tcatctgttc gttttgctct cagtttccgt
     1801 taccccggct gggcaaaaat gatgattatt aatggtgtta cttttactaa aaatgatact
     1861 caaaactcac ttattatttt agatagaaca tggcaacatc atgatgtgat tgacattaaa
     1921 tttatagctg atatacagtt taatactgac ttatgtggtg acacttatat ttccagaggt
     1981 cccgttttat atgcaattga gcttgagagc gatattttaa tcaaaaaaaa cctgctgaac
     2041 gggaaatatt ttgactcagg atacataccc tgtagtagag ctgatgaatc tatccagttc
     2101 tcttatgctg atatggaatc tttttcatat tcaacctcac cagagggcaa acaatacatt
     2161 gaaggctgtt tttatttaaa caagactaaa atagtcagaa aacttatacc atttggtcaa
     2221 accattcttc gcaaagtaac tttctccaat atagagagtt aatattttcg agtgttataa
     2281 ttaattaaag ggatttctat gtaccttact gataatatta tcaaactgtc gaataaaata
     2341 ggtcagcgtg tttatactgt tgtctctgaa atgtcgattg aagcatggat aacaaaagaa
     2401 ccgcagcctt ttgtttatcg ccgtagcggc gcttaccaga aactcaccat tggctcagac
     2461 tggggtcaac tgtttgactg tgcctggatg cgagtttatg gtaaacgtcc agcagataaa
     2521 tttagacaca aactcgtcgc tctcgttgat atcggtggcg aaggtcttat tgtggataag
     2581 aatggttcac ccatctgcgg catcacgaat aaagcatcat cttatggcgt accgccagat
     2641 aaaccaggta aatgggtggt tgacctttct ctcgttagtg aaaatgacga ggttgagttt
     2701 tggattgatg ctgcctgcaa cgatttattt ggctatgtta ctaatggagg gattattact
     2761 gatgttcata tcgcgacacg taatcaacta ctgaaatccc tttattatga tattgaggtg
     2821 cttttcgact ggattaatga tggtcagaag tttgaatcca tacatcctaa aggtatcacc
     2881 ccagaacaat ttattgccaa aagaagtgag cgtactgacg aaatcataag tactctcgaa
     2941 tatattgata atacgttaat tacctttagc aatgaagaaa taattaaatg tcaaattgca
     3001 atccaatcca taatcaataa aaacaataat ccttcagagt tcaggattat ggctactggt
     3061 catgcccatt tagatatagc gtggatgtgg cctttaagag aaggacgcag aaaagcaata
     3121 agaacatttg ccaccgccct tgcaaacatt gaaaaatatc ctgattacat ttttggcgct
     3181 agtcagtacc aactttttca ctggataaaa aaagattatc cctatttctt cgaaaagtta
     3241 aaaaaacaaa tcgccgcagg ccgctttgag ttacaaggtt gcttctgggt cgaatgcgat
     3301 ttaaatctgg taagtggtga atcgctgata aggcaaatca tgcatgggac acgattcaca
     3361 ctgcagaatt tttcaaaaaa gataaattat gtctggcaac cggatgtctt tggattcccc
     3421 gcaacgcttc cacaaattct caaaaaatca ggcagtaact atattgcatc gcaaaagctc
     3481 tcacaaaaca agattaataa atttaataat taccttttcc gctggcaggg cctggatggc
     3541 tcagaaattc tgatgcataa ctttcctgaa gacacttatg atagccgagc acgtgctcgc
     3601 tctttagaat atattgaaca aaactataat gaaaaagaga tctgtcccta tgcgcttatg
     3661 gtgtatggtg tatggtgtcg gtgatggcgg cgctggcccc ggtgaggaac atatagaacg
     3721 attaacacgt atacgcaata ttgatggctt acctcatgtt gatttcagcc gtgttgataa
     3781 gttcttcact tacgccgacg cctttcgcga aagcttgccc attatttcag gtgaacttta
     3841 tttcgaggct catcaagggt gctttacatc agagtcagca actaaagctc ataatcgaaa
     3901 tatggaaaat aaattacatg atgctga
//
LOCUS       DAZPLI010000257         3908 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZPLI010000257 DAZPLI010000000
VERSION     DAZPLI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.260"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            24..590
                     /locus_tag="ACHUU3_004170"
     CDS             24..590
                     /locus_tag="ACHUU3_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR2084082.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            594..1169
                     /locus_tag="ACHUU3_004171"
     CDS             594..1169
                     /locus_tag="ACHUU3_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR2084083.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1211..2263)
                     /locus_tag="ACHUU3_004172"
     CDS             complement(1211..2263)
                     /locus_tag="ACHUU3_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR2084084.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2488..3408
                     /gene="lpxL"
                     /locus_tag="ACHUU3_004173"
     CDS             2488..3408
                     /gene="lpxL"
                     /locus_tag="ACHUU3_004173"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR2084085.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3580..3765
                     /locus_tag="ACHUU3_004174"
                     /pseudo
     CDS             3580..3765
                     /locus_tag="ACHUU3_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
BASE COUNT          971 a          987 c          952 g          998 t
ORIGIN      
        1 gcaaattcgt attggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta
       61 tatctgcggc cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc
      121 tgtggatggt cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata
      181 aaagcatcgg tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat
      241 ctccaccgcc gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaggctg
      301 gacatctcgc attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa
      361 ctgccgatgg taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg
      421 acgctggcgc acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg
      481 tagtactgtc cgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg
      541 acactctgaa gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa
      601 aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg
      661 ccgattacaa aattgacaaa gaaggtcagc acgcctttgt taatttccgc atccagcacc
      721 ttggctatag ctggttatac ggcaccttta aagatttcga cggtactttt acctttgacg
      781 aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta
      841 atcacgccga acgcgataaa catcttcgca gtgcagattt cctcaatacc gcaaaatatc
      901 cacaggcaac attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg
      961 gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc
     1021 agggcgacga cccatggggt ggtaaacgtg caggcttcga ggccgaaggc aaaattaagc
     1081 tcaaggactt caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta
     1141 tttcagtgga aggcgtacag cagaagtaag cgtgaaatgt tgccggatgg cacgcatccg
     1201 gcaatgatat ttattctgtt ggatcaggaa tgcccagtgt tgtattcaga cgtccacgag
     1261 acttattaaa gatcttattg ccattttcac gtcctgcccg acggcgtcgc tgttcttctg
     1321 gcggtaacgc gctttcttcg cagcaaatct cactacaaca acctttgtat ttttccgcgc
     1381 atactggaca ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt
     1441 cgcacggcgc gccgcactgg tggcaatgcg cgataatctc atccgatata cgttcgccca
     1501 tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg
     1561 ccttacgggc gtattcaata attccgccct cgatatgcca gactttgttg aatccgttat
     1621 gtttcatcca ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt
     1681 ttttatcttt atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat
     1741 ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca
     1801 tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat
     1861 actcaccaac attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat
     1921 cgcgtacctt catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca
     1981 ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat gtttcaacat
     2041 tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa
     2101 aaacattcag cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga
     2161 tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc gctctcaacc aacattttgg
     2221 cttttaacgc gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg
     2281 agatgggaaa gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacac
     2341 attttacgct acatttgcgc attaaaaatt atttgttatt tacaagcgcg gcaatttcgc
     2401 ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc
     2461 ccgaataggt tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg
     2521 cttcatccgc gttattggtt aacctggttg ggtattggcg tactttggtt agtcgtgcaa
     2581 ttgccctacc cggttatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt
     2641 atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc
     2701 gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggactgatg
     2761 gaaaccggta tggcgtggtt ctggccggac cgccgaatcg cccgctggac ggaagtgatc
     2821 ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc
     2881 cattttctga cactggaact gggcgcgcgg cagtttggta tgcaggaacc ggggattggc
     2941 gtttaccgcc cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc
     3001 tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa
     3061 ggcgaagtgg tctggtacgc accggatcat gattacggcc cgcgctcaag cgttttcgtc
     3121 ccgttatttg ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg
     3181 tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa
     3241 ttgattatgc tgccgccaga gtgttctccg ccactggatg atgccgaaac taccgccgcg
     3301 tggatgaaca aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta
     3361 caccgtcgct ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccctgcc
     3421 ggatgcttca gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa
     3481 gctctctgga ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta tcttttcttc
     3541 ttatgttcac cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tggaaatgac
     3601 acccctataa actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt
     3661 gccgccttca gtctggtaat gcccttctta cccctctacg ttgagcagct tggcgttacc
     3721 ggtcactccg ccctgaatat gtggtccggt attgtcttca gtataatacc ttcctaaata
     3781 cactacctga gagaagagat gaaaagagct atcagctaca tgaggttttc aactgagaag
     3841 cagataaaag gggactctat acgaagacag tctaagctgg tcactgactg gttatcaaag
     3901 aaccctga
//
LOCUS       DAZPLI010000258         3847 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZPLI010000258 DAZPLI010000000
VERSION     DAZPLI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.261"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            69..1055
                     /gene="dcyD"
                     /locus_tag="ACHUU3_004175"
     CDS             69..1055
                     /gene="dcyD"
                     /locus_tag="ACHUU3_004175"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR2084086.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            1070..1738
                     /gene="tcyL"
                     /locus_tag="ACHUU3_004176"
     CDS             1070..1738
                     /gene="tcyL"
                     /locus_tag="ACHUU3_004176"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR2084087.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            1735..2487
                     /gene="tcyN"
                     /locus_tag="ACHUU3_004177"
     CDS             1735..2487
                     /gene="tcyN"
                     /locus_tag="ACHUU3_004177"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR2084088.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            2717..3439
                     /gene="sdiA"
                     /locus_tag="ACHUU3_004178"
     CDS             2717..3439
                     /gene="sdiA"
                     /locus_tag="ACHUU3_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFR2084089.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(3506..3730)
                     /gene="yecF"
                     /locus_tag="ACHUU3_004179"
     CDS             complement(3506..3730)
                     /gene="yecF"
                     /locus_tag="ACHUU3_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR2084090.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(3717..>3847)
                     /gene="yecU"
                     /locus_tag="ACHUU3_004180"
     CDS             complement(3717..>3847)
                     /gene="yecU"
                     /locus_tag="ACHUU3_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR2084091.1"
                     /translation="VDVTLKKQPLLFLAKLMVYSAALTFFTANFHCNMTRKINEYA"
BASE COUNT          938 a          907 c         1022 g          980 t
ORIGIN      
        1 ccttttttat ttacgcgttt tcagcgtgca taataagaaa atcacaacaa catacatcgg
       61 aggctgttat gccactgcat aatttaaccc gttttccacg gttggagttt atcggcgcgc
      121 caacgccgct cgaatatctg ccgcgctttt ctgattatct aggacgggaa attttcatca
      181 aacgggatga cgtcaccccc atggcaatgg gcggcaataa attacgtaag ctagaatttc
      241 tcgcggcaga tgctctgcgc gaaggtgccg atacgctgat tactgccggg gtgatccagt
      301 ctaaccatgt gcggcagact gccgcagttg cggcgaaact cggtctgcac tgtgtggcgc
      361 tgctggaaaa tcctattggc acaaccgcag aaaactattt aaccaacggc aatcgcttgt
      421 tgctggatct gttcaatacc cagattgaaa tgtgcgaagc actgaccgat cccaatgccc
      481 aactggaaga gctggcgacg cgagtcgaag cacaaggctt tcgcccgtat gtcattccgg
      541 ttggcggttc taatgctctt ggcgcgctag gttatgtgga gagtgcgctg gaaatcgcgc
      601 aacagtgtga aggggcggtt aatatttcgt cggtggtagt cgcatcgggc agtgccggaa
      661 ctcacgccgg actggctgtt gggctggaac acctgatgcc tgaaagcgaa ctgattggcg
      721 tgaccgtgtc gcgttccgtt gccgatcagt tgccgaaagt ggttaaccta caacaggcga
      781 ttgcgaaaga actggagctg accgcatcag cggaaattat actctgggat gactattttg
      841 cacctggcta cggcgtgccg aacgacgaag gcatggaagc agtgaaattg ctggcgcggc
      901 tggaaggcat tctgcttgat cctgtgtata ccggaaaagc gatggcgggg ctgattggct
      961 gtatcagtca gaaacgcttc aaagatgaag ggccgattct gtttattcat accggcggtg
     1021 cgcctgcgct gttcgcctat catccccacg tttagctccg ggaacgctca tgcaagaaag
     1081 tatacaactg gttattgatt ccttgccgtt cctgctgaaa ggggctgggt atacgctgca
     1141 actcagtatc ggcggcatgt tttttggctt actgctcggg tttattctcg cgctgatgcg
     1201 cctgtcgcca atctggcccg tgcgctggct ggcgcgtttt tatatctcta tttttcgtgg
     1261 tacaccgctc atcgcacaac tgtttatgat ctattacggc ctgccgcagt ttggtatcga
     1321 attagatccc attccgtcag caatgattgg tctgtcgctg aacactgccg cctatgccgc
     1381 cgaaacactg cgcgccgcca tttcttctat tgataaaggt cagtgggaag cagctgccag
     1441 tattggtatg acgccatggc agactatgcg tcgcgcgatt ttgccacagg cggcgcgggt
     1501 ggcgttgccg ccgctgtcga acagctttat cagcctggta aaggatacgt cgctggccgc
     1561 gacaatccag gtgccggagc tgttccgtca ggcgcagttg attacctcgc gcacgctgga
     1621 ggtgttcacc atgtacctgg cggcttcgct catctactgg atcatggcga cagtattatc
     1681 gacgttgcag aaccattttg agaatcaact taatcgccag gagagagaac caaaatgagt
     1741 gccattgaag ttaagaacct ggtgaaaaaa tttcacggtc agacggtgct gcacggtatc
     1801 gacctcgagg taaagcctgg cgaagtggtg gcaattatcg gtccgagtgg ttccggcaaa
     1861 accacgttgc tacgcagcat aaatctgctg gaacaaccgg aagcgggaac gatcaccgtt
     1921 ggcgatatca ctattgatac tgcacgttca ttaagtcagc aaaaatcgct gatccgccag
     1981 ttgcgtcagc acgtcgggtt tgtcttccag aactttaatt tgtttccgca tcgtacggtg
     2041 ctggaaaaca ttattgaagg gccggtgatc gtcaaaggcg aaccgaaaga agaggccacg
     2101 gcgcgcgctc gcgagctgct ggcgaaagtt gggctggccg gtaaagaaac cagctatcca
     2161 cgtcgtttgt ctggcggtca acagcagcgt gtagcgattg cgcgtgcgct ggcaatgcgt
     2221 cctgaggtga ttttgtttga cgagccaacg tcagcgctgg atccagagct ggtgggcgaa
     2281 gtcctgaaca caatccgcca gctggcgcag gaaaagcgca cgatggtgat tgtgacgcac
     2341 gaaatgagct ttgcccggga tgttgcggac cgggcgattt ttatggacca ggggcggata
     2401 gtcgagcagg gggcagcaaa agcgttattt gctgatcccc agcagcctcg cacccgccag
     2461 ttcctcgaga agtttctgct gcaataataa gaaaaaatca gccccgacga ttcacctgtc
     2521 ggggcgggac gccatttcaa gcgtgataaa actgcttaac aaatcagcat aactcattaa
     2581 taacataaga gaatgcgatg gcttgcaaaa gtaattcatt gcctgaataa tataaattat
     2641 atataaatct tatttatgtg atagtttgaa ttatcattat aaatgatact cactctcagg
     2701 ggcgttgcgg tttactatgc aggatacgga ttttttcagc tggcgtcgca cgatgctgtt
     2761 gcgttttcag aggatggagg ccgcagaaga ggtctaccat gaaatagagc ttcaggctca
     2821 gcagctggag tacgattact attcgttatg tgttcgccac ccggtaccat tcacgcgacc
     2881 taaagtggct ttctacacca attaccctga gtcgtgggtt agttattatc aggcaaaaaa
     2941 ttttctcgca attgatcctg tgctgaaccc tgaaaacttt agtcagggcc atttaatgtg
     3001 gaatgatgac ttattcagcg aagcacagcc attatgggaa gccgcgcacg cacatggttt
     3061 acgccgcggt gtcactcagt atttaatgct gccaaatcgg gcgctgggct ttttgtcctt
     3121 ttcccgttgc agcacgcgcg aaatacccat tcttagtgat gaactgcaat taaaaatgca
     3181 gttactggtg cgcgaaagtc tgatggctct gatgcgttta aatgatgaaa tagtgatgac
     3241 gccagagatg aatttcagca agcgcgaaaa agaaattctg aaatggacgg cggaagggaa
     3301 gacatcagca gagatagcga tgattttgtc aatctctgag aatacggtca atttccatca
     3361 gaaaaacatg cagaaaaaaa tcaatgcacc aaataagacc caggtggcct gttacgcggc
     3421 cgctactggc ttaatttgat cgcttttctt tctgcgtgcc agatgcaaaa atcggctgaa
     3481 aggcacgctc tcagccggtt ttatattact gacggtaggc ttgtttaatt tgcttaaccg
     3541 ttttggaaaa tactgcagcc tgggtttcgt cttcgatcag cgcaagctgt ttttccatct
     3601 ttaacatcac gcggcccgcg tcagcttgtc ccatcgcctg tagcatcagc gtcagcatcg
     3661 ctttcaggca ggagacttca tttgccagtt cttgattatt ctcggcagta gaaaaatcag
     3721 gcgtactcat ttattttcct cgtcatgttg caatgaaaat ttgcggtgaa aaatgttaag
     3781 gcggcggagt ataccataag ctttgctaaa aatagcagtg gttgtttttt gagcgtgacg
     3841 tcaacaa
//
LOCUS       DAZPLI010000259         3832 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZPLI010000259 DAZPLI010000000
VERSION     DAZPLI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.262"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..265)
                     /locus_tag="ACHUU3_004181"
     CDS             complement(<1..265)
                     /locus_tag="ACHUU3_004181"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR2084092.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
     gene            352..759
                     /gene="nudG"
                     /locus_tag="ACHUU3_004182"
     CDS             352..759
                     /gene="nudG"
                     /locus_tag="ACHUU3_004182"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR2084093.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(725..997)
                     /locus_tag="ACHUU3_004183"
                     /pseudo
     CDS             complement(725..997)
                     /locus_tag="ACHUU3_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
     gene            1233..2576
                     /gene="gdhA"
                     /locus_tag="ACHUU3_004184"
     CDS             1233..2576
                     /gene="gdhA"
                     /locus_tag="ACHUU3_004184"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR2084094.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2693..3733)
                     /locus_tag="ACHUU3_004185"
     CDS             complement(2693..3733)
                     /locus_tag="ACHUU3_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR2084095.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
BASE COUNT          999 a          932 c          916 g          985 t
ORIGIN      
        1 caagagaaat atcaagaaag ccgcccgcgt cggtaagctc tctgcgccgc gccagcgcac
       61 cgtccagacc atcaagcagc ctgttcaaca aaatgacgac tagcgccgcc agataccagc
      121 ccagcgccag aaacggcaga gccagcacgc cgatggcaaa tcccactaat gttaaaccat
      181 ctggcgtaat gcccggttta tcaagaaccc gcacgcactg atgcaacaac ggtttaatcc
      241 ggggatgaag atggcggtct agcacaggca ctccttaaat ataaagcctt tctgattgag
      301 caacagtgcg gatattatgg catttttcgc ttatctgccc gtgtgtaatt tatgaaaatg
      361 attgaagttg ttgccgccat cattgaacgt gatggcaaaa ttttactcgc gcaacgcccc
      421 gcccagagcg atcaggcggg attatgggag tttgccgatg gtaaagtcga gctggatgaa
      481 agccagcagc aggcactggt gcgtgagtta aacgaagaat tgggcatcga agcaactgtg
      541 ggtgagtatg ttgccagcca tcagcgagaa gtttcgggcc ggattatcca tcttcatgcc
      601 tggcacgtac ccgacttcca cgggacgtta caggcacatg aacatcaggc gctggtctgg
      661 tgctcacctg aagaggcgct gcaatatccg ctggcccctg ctgacattcc actattagag
      721 gcgtttatgg ctttacgcgc cgccagagca gcggattagt gctaagggtt ttgtcatccc
      781 gctggcatta cagcagtatt ccttcggctt taattaccgc cccttcagaa taattttgat
      841 cctgataaac gcagcactga gtacagggct gcgctgactg cccgcctgaa ctgaacactt
      901 ctggcggtac gtttacctcc acgtccggac gataatgcgg gttagccagt gcaattaatg
      961 gaaatgctaa tactacggcg aacaatgctc gactcacagg gaactcctta acgttattgt
     1021 ctctgcaact gataacggta gccgggtggc aaaactttag cgtctgagtt atcgcatttg
     1081 gttatgagat tactctcgtt attaatttgc tttcccgggt catttttttc ttgcttaccg
     1141 tcacattctt gatggtatag tcgaaaactg caaaagcaca tgacataaac aacataagca
     1201 caatcgtatt aatatataag ggttttatat ctatggatca gacatattct ctggagtcat
     1261 tcctcaacca tgtccaaaag cgcgacccga atcaaaccga gttcgcgcaa gccgttcgtg
     1321 aagtaatgac cacactctgg ccttttcttg aacaaaatcc aaaatatcgc cagatgtcat
     1381 tactggagcg tctggttgaa ccggagcgcg tgatccagtt tcgcgtggta tgggttgatg
     1441 atcgcaacca gatacaggtc aaccgtgcat ggcgtgtgca gttcagctct gccatcggcc
     1501 cgtacaaagg cggtatgcgc ttccatccgt cagttaacct ttccattctc aaattcctcg
     1561 gctttgaaca aaccttcaaa aatgccctga ctaccctgcc gatgggcggt ggtaaaggcg
     1621 gcagcgattt cgatccgaaa ggaaaaagcg aaggtgaagt gatgcgtttt tgccaggcgc
     1681 tgatgaccga gctttatcgt catctgggcg cggataccga cgttccggca ggtgatatcg
     1741 gcgttggtgg tcgtgaagtc ggctttatgg cggggatgat gaaaaagctc tccaacaata
     1801 ccgcctgcgt cttcaccggt aagggccttt catttggcgg tagtcttatt cgcccggaag
     1861 ctaccggcta cggtctggtt tatttcacag aagcaatgtt aaaacgccac ggtatgggtt
     1921 ttgaagggat gcgcgtttcc gtttctggct ccggcaacgt cgcccagtac gctatcgaaa
     1981 aagcgatgga atttggtgct cgtgtgatca ctgcgtcaga ctccagcggc actgtagttg
     2041 atgaaagcgg attcacgaaa gagaaactgg cacgtcttat cgaaatcaaa gccagccgcg
     2101 atggtcgagt ggcagattac gccaaagaat ttggtctggt ctatctcgaa ggccaacagc
     2161 cgtggtctgt accggttgat atcgccctgc cttgcgccac ccagaacgaa ctggatgttg
     2221 acgccgcgca tcagcttatc gccaatggcg ttaaagccgt cgctgaaggg gcaaatatgc
     2281 cgaccaccat cgaagcgact gaactgttcc agcaggcagg cgtactgttt gcaccgggta
     2341 aagcggctaa tgctggcggt gtagcaacgt cgggcctgga aatggcacaa aacgctgcgc
     2401 gcctgggctg gaaagccgag aaagttgacg cacgtttgca tcacatcatg ctggatatcc
     2461 accatgcctg tgttgagcat ggtggtgaag gtgagcaaac caactacgtg cagggcgcga
     2521 acattgccgg ttttgtgaag gttgccgatg cgatgctggc gcagggtgtg atttaagttg
     2581 taaatgcctg atggcgctac gcttatcagg cctacaaatg ggcacaattc attgcagtta
     2641 cgctctaatg taggccgggc aagcgcagcg cccccggcaa aatttcaggc gtttatgagt
     2701 atttaacgga tgatgctccc cagggaacat ttcttatggg ccaacggcat ttcttactgt
     2761 agtgctccca aaattgcttg tcgtaacgat aacacgcttc aagttcagca tccgttaact
     2821 ttctgcgata gcagcagata tgccagtaaa gaaaccccat tagactattt ttttgataat
     2881 cttcttcgct ttcgaacaac tcgtgcgcct ttcgagaagc aagcattata taatgccagg
     2941 ccagttcttc ttcaattgtc ccgttttgaa aagctgtgct tgatatcgag gtcatccatg
     3001 ataattccgc cgcccatatt agcttcgccg aggatttacc ggagcgatta ttagcgcaat
     3061 cagagatata gtctgaggga aaaacagcaa atttattcaa caaggcgata acctgctctg
     3121 gggcttcctc catgtttgct ttaaaggtat tggctccatg gtcgccagaa agaaaatgct
     3181 ccattaaggc acaataactt tcgctatctt cgatacccca ttgattctct aaagactcgc
     3241 gtcttttact gatgatatcg atcgagtcaa aaggaagcac atgatattgg aaggtatctt
     3301 taccaggttc aggctttcgc ggccagaact ctagcgtttc agaccattgc ttatgataga
     3361 atcgataagg tgcgatcaat tgtagcgcct gtaacttctc gatactgagc ggctcaatac
     3421 ctttagcctg ataataatgc agttgttctt tttttgcttt aaaaccggcc cgaacaataa
     3481 gccccatcat aattaataga taaagaaaag agcatcccgc ggtaatcagg cctctttcat
     3541 tcaaaccgtt ggatgttatc gctgcgaaca caaacattac agcgacaaca catgttaaat
     3601 aaaaccccca cttacaaagc agcatggcct tattttcttt aatcatccgt tcaaaattac
     3661 tattaaatat ttcccagcca ttaaaagaat acttctcgct cccaggatgg ttttgtaata
     3721 aaactttttt catcacattc cctgttatta catactttag aggcgataat tttatccatg
     3781 caaaaaaaat atccaacgaa ttttcttgat ccgctggata tttcagaata tg
//
LOCUS       DAZPLI010000260         3808 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZPLI010000260 DAZPLI010000000
VERSION     DAZPLI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.263"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..1358
                     /gene="dcuC"
                     /locus_tag="ACHUU3_004186"
     CDS             <1..1358
                     /gene="dcuC"
                     /locus_tag="ACHUU3_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR2084096.1"
                     /translation="VVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSSQ
                     ASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQY
                     INSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASPA
                     AIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEHI
                     SHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILEF
                     LRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSFG
                     SASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQASN
                     LGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVTG
                     K"
     gene            complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="ACHUU3_004187"
                     /gene_synonym="citB"
     CDS             complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="ACHUU3_004187"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFR2084097.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="ACHUU3_004188"
     CDS             complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="ACHUU3_004188"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFR2084098.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLNVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT          880 a          988 c          917 g         1023 t
ORIGIN      
        1 gggttgtggt tattgtgggt gtagctcgct acatcattaa agggtattcc gccactggtg
       61 tgttatttgt cggtggcctg ttattgctga ttatcagtgc cattatgggg cacaaagtgt
      121 taccgtccag ccaggcttca acaggctaca gcgccacgga tatcgttgaa tacgttaaaa
      181 tattactaat gagccgcggc ggcgacctcg gcatgatgat tatgatgctg tgtggatttg
      241 ccgcttacat gacccatatc ggcgcgaatg atatggtggt caagctggcg tcaaaaccat
      301 tgcagtatat taactcccct tacctgctga tgattgccgc ctattttgtc gcctgtctga
      361 tgtctctggc cgtctcttcc gcaaccggtc tgggtgtttt gctgatggca accctatttc
      421 cggtgatggt aaacgttggt atcagtcgtg gcgcagctgc tgccatttgt gcctccccgg
      481 cggcgattat tctcgcaccg acttcagggg atgtggtgct ggcggcgcaa gcttccgaag
      541 tgtcgctgat tgacttcgcc ttcaaaacga cgctgcctat ctcaattgct gcaattatcg
      601 gcatggcgat cgcccacttc ttctggcaac gttatctgga taaaaaagag cacatctctc
      661 atgaaatgtt agatgtcagt gaaatcacca ccactgctcc tgcgttttat gccattttgc
      721 cgttcacgcc gatcatcggt gtactgattt ttgacggtaa atggggtccg caattacaca
      781 tcatcactat tctggtgatt tgtatgctga ttgcctccat tctggagttc ctccgcagct
      841 ttaataccca gaaagttttc tctggtctgg aagtggctta tcgcgggatg gcagatgcgt
      901 ttgctaacgt ggtgatgctg ctggttgccg ctggggtatt cgctcagggg cttagcacca
      961 tcggctttat tcaaagtctg atttctatcg ctacctcgtt tggttcggcg agtatcatcc
     1021 tgatgctggt attggtgatt ctgacaatgc tggcggcagt cacgaccggt tcaggcaatg
     1081 cgccgtttta tgcgtttgtt gagatgatcc cgaaactggc gcactcttcc ggcattaacc
     1141 cggcgtattt gactatcccg atgctgcagg cgtcaaacct gggtcgtacc ctatcacccg
     1201 tttctggcgt agtcgttgcg gttgccggga tggcgaaaat ctcaccattt gaagtcgtaa
     1261 aacgcacctc ggtgccggtg cttgttggtt tggtgattgt tatcgttgct acagagctga
     1321 tggtgccagg aacggcagca gcggtcacag gcaagtaaat agtaatgccg gaggcaagtt
     1381 tcctccggct ttatcatgtc acccactgtg gtatatgcgt tgtggtctgc caactttgcc
     1441 gtgaacaatt tcagcaataa tcagatggcg gctggcgcaa tattcaagat aacgcctggc
     1501 agtggtgcag ctgatggtca gtgcctgcgc caccgtttct gccgtatgtt gcacaccagg
     1561 ctctttaaac agttttcgca ccgcgtttag cgtcaagggg tcaatgccgg tcggtagctc
     1621 gtccttaggt tcaccgcgag cataagcatt aaacatctca tcaatttgct tctggctggc
     1681 gctatcaata ctttccagca tatgtttacg ctggcggaaa cgggttagcg tttgccccag
     1741 ccgttcatag gcaatgggct taatgagata atcaaataca ccacaacgta cggcttcaga
     1801 caccgtttcc atatcgctgg ctgcagtggt aaacaccacg tcgccgggat aatgcgcctg
     1861 caccagttca tgcagtaaat taatccctct accgtcagga agatagttat cgagcaagat
     1921 tagccccggc ttaaaacgct cgatcatcat tcgggcctgc gccagatttc ccgccagtaa
     1981 tatctgactg aatccgggaa tgtgacgaat atattccgca tgcatctctg ccagcggcgt
     2041 ttcgtcctca acgatcaata gggttaatgg agctgtcatt aggtttcact ttcggaatat
     2101 agattgaaaa taaggtaccg cagggatcat tatcttcgag agtgataaca ccaccgcagc
     2161 gcgttacgta gctggcaatc aagtacaacc caatgccatg ttcaccgggc tcgtcagcac
     2221 gcgtactgac cccctgctca aatattttgt ctcttagaga ctctggaacg ccgcagccct
     2281 gatcggcgac ttcaatcacc acatcatcgc cttcatcgct gaggaataat tcaacgatct
     2341 tgtttccttc atcgctacgc aggctggctt cgaaggcgtt atcaagtaaa ttgccaacaa
     2401 tggcggcaaa ctcggtgcta tccagtcctg gcggcagttg cgaaagctgg ctaccgggga
     2461 cgatgatcat ttttagcccc agttcgcggg cgcgctgcac tttaccaaaa agcagccccg
     2521 ccacctggcg atcggcaaac gcctcacgca ggctgtcaat aagctgttgc tgggcctgag
     2581 actccccctg caccatggcc agcacgcgat catactcttt catctgcaac agaccattga
     2641 gcgtcgacat ccaattgaga tgctcgtgtc gcaatgtacg aaggctctcg acgtattgtt
     2701 ttatttgcgt cagttgcgca ttgagggtgg aaatttcgtc tttactacga aagctgataa
     2761 tggcccccag caaatcatca cctgaacgaa tagcttcccg gttggcaata acgttcagac
     2821 cattaaagtt cgccaccaca tcctgacgtt tttcatcaat ctgttcggta aagaaatcgg
     2881 cgggcctgac cacttcagca atgggtttac ccaaccattg ccgtccggga gagctcagcc
     2941 ccagcatctt tcttgcgtta cgattgatgg cggtaatgta accatgcgga tccaccgcaa
     3001 tcagcccttc ataaaccgaa ctaaacagcg cctcttgctg acggaccacg cgtgcgattt
     3061 gctttggctc catgcccatc atttgtcgac ggatatgcgc ggccaggaac cacgacagca
     3121 acatcagaat ccctaacagc acgacaaaca cgcctgccat cggtaataaa aactcagccc
     3181 gccagctatc gattttactc accaggtagc caatcgacac cacgccgatg acttttccat
     3241 cgtcatcaaa gattggcgtt ttggcgcgca tcgccatccc cattgcccct ttcccggtaa
     3301 tgaagtagct ctcccctttc tccagcgcgc cctgtttggt gaactgcata ggataaccaa
     3361 ttttctccgg attaagatgg taaaggcgga tcgagtgccg gtcgccaatc accacataat
     3421 caaaatcggt atctctttgt aatttgttag cgatggtcgc cagccgtttg tagtcacgcg
     3481 ttttcaccgc agagatgaca ctgtcattgg aggcaataat tttcgcctga ttcattgcca
     3541 tgtcgcgtac atgaagcgtt aaatagtcct caaaactggc cgtaaaatat tgcgccaggg
     3601 ctgcaatgac aaatattgag aacaccagaa tcagcaaaaa gatacgcagc ggaaatgcca
     3661 gtctttggaa aaatgcaaac tgtttattct cgttaagctg caacattgtt ttccttgcgg
     3721 gttgtcccga gcctctttga cggatgtaat taaaatgttg aatcaaaata catagatatt
     3781 aattatgatc gtggcaaatt ttattcat
//
LOCUS       DAZPLI010000261         3737 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZPLI010000261 DAZPLI010000000
VERSION     DAZPLI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.264"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..182)
                     /locus_tag="ACHUU3_004189"
     CDS             complement(<1..182)
                     /locus_tag="ACHUU3_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFR2084099.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            426..683
                     /locus_tag="ACHUU3_004190"
     CDS             426..683
                     /locus_tag="ACHUU3_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFR2084100.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(733..1683)
                     /gene="uspE"
                     /locus_tag="ACHUU3_004191"
     CDS             complement(733..1683)
                     /gene="uspE"
                     /locus_tag="ACHUU3_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR2084101.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="ACHUU3_004192"
     CDS             complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="ACHUU3_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR2084102.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="ACHUU3_004193"
     CDS             complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="ACHUU3_004193"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR2084103.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3308..>3737)
                     /locus_tag="ACHUU3_004194"
     CDS             complement(3308..>3737)
                     /locus_tag="ACHUU3_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR2084104.1"
                     /translation="KFIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWS
                     ILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLG
                     TYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1003 a          887 c          893 g          954 t
ORIGIN      
        1 ataatgctac caacagcact gataattatg gtgtagatta aaaattgcac cgcatggaat
       61 aaaaaacctt ttcttttacg atttccgcgg cgaaaccaaa agctcatcag aatcaatgct
      121 gcgcagctac cgaaaataat gaccagatta agcgcattat ttgtaaacag ttcagcgatc
      181 attgttttat caggctcctc cagataattg tcgtcatgcc ggaaacccct ggcggggcta
      241 ttttaccgcg acaattcatt cagatcatca atagtcaggg aaggaagtag caacattagc
      301 taactaatca atgcgttgat tgtaaatcca gctaagaggt gaggttttca gagcagacaa
      361 cggtgaaatg tcatggtatt gttacgttta ggtaacaaga aatttgtctg cacaaggatt
      421 acatcatgat tatggcgaaa ctgaagtcag cgaaagggaa aaaatttctc tttggtttgt
      481 tggcggtttt cattattgcg gcgtcggttg tgactcgcgc gactatcggc ggcgttatag
      541 aacagtacaa tattccgctg tctgagtgga cgacatcaat gtatgtgatt cagtcatcga
      601 tgatttttgt ttatagcctg gtctttactg tgttgctggc aatcccgttg ggaatttatt
      661 tccttggcgg cgaagagcag taagtaaaaa ataggcccga taactcgggc cttgtcagtt
      721 attgaagagt cgttaatcgt cttcttcgtc atccagttca acgggtgtct gatactggtc
      781 aggtttaatg accaacagat cgcagcgaag atgatcaatc acctgttccg ccgtgttgcc
      841 gaggaatgct gctgaaatac cggtgcgtcc taccgtgccc agaaccacaa tccccgcctg
      901 taagtgctcc gccaaatcag gaataacctc ttccggcaga cctttttcta cgtgcgtcat
      961 gttttcatta atgccgaatt tctgccgcag agctttcatt gccagcaaat gttgcccacg
     1021 aatggcatcg ttataaacgc tcggatcaaa ttccggcagt tcaatcgcga tattaattgg
     1081 cgttactggg taagcgccaa ccagatgaac ttcggtatgg ttgacttgtt ctgccagttc
     1141 gatcgtctct ttgaccagtt tttcattgag cgcattatga tacggctctt cactggcgag
     1201 attcaccgcc accagcgcct tacctccttc cggccacggc tggtctttca ccatccacac
     1261 cgggcttggg catttgcgta acagatgcca gtccgttggc gtaaaaatca ccgcttccag
     1321 acggtcatgt tggtgcgcca tttttagcac caaatcgtgc ccgccgctga tcacttcctg
     1381 aatgatggct tcgaagggac ggttatgcca gaccacttta atttcaatgg gaacgccagc
     1441 attgagataa tattttgcct gctcgtggat ccaggctgta cgctggctga tgacgccctg
     1501 acgcatagcg gtacgttcgt ccggggagag cagggtggtc atttcgtatg agaagtcata
     1561 gatcggcaaa aaggctttaa ttttgccacc aatccgttga tgtaaataaa cagctcgccg
     1621 caatgctggt tggtcgtcct ggttaggatc gataacaacg agcatgttct gatacatagc
     1681 catacagggt ctccttacaa caactgtcaa cgcagtttgt aattaaaaga ttaacccata
     1741 tctggtgaat gaaacagtga tgaaccttct gccagatcaa taaatcagaa aaatttaatg
     1801 atatgacaga aggatagtga gttatgcgga aaaatcaggc aacattacgc gtatgaccag
     1861 caagctgggc cagcgcatcg ttattttcga tggtgatgta tttacctttg actgccagca
     1921 taccgctttt ctggaagcga cctagcagac ggctgatggt ttctaccgtc aggcccagat
     1981 agttaccgat atcgccacga gtcatcgtca ggcggaattc acgaggggag aagccgcgtt
     2041 gggcaaaacg acgggacagg ttgtagatga atgcagccag acgttcctcg gcatttttct
     2101 tcgacaacag caagatcatg tcctgatcgc ctttgatttc accgctcatc agacgcatca
     2161 tctgctgacg cagattcggc attttaccgg acaaatcgtc cagcgtttcg aacgggattt
     2221 cacataccat cgaggtttcc agcgcctgag cgaagctcgg gtgatggccg ctgccgatgg
     2281 cgtcaaatcc caccaggtcg ccagctaaat ggaaaccagt gatttgctcg tcgccttgct
     2341 cagtgatggt ataactttta atcgtaccgg agcggatggc ataaagcgat ttaagttcat
     2401 caccagcctt aaacagcgtc tggcctttct gaataggctt cttccgctca atgatattat
     2461 caagctgatc aagctcatgt tcgttgagtg tgaacgggat gcaaagctgg ctgatgctgc
     2521 aatcctggca atggatagca caaccgccag actgaatgcg ccgtataatt cgcttttccg
     2581 ggatcatagg tctgctcaag ccgtaattga tatttgtcaa ttttaacatc tttttaggga
     2641 gcaagtaagt ctaagcaaac cttaacagca gagaattccg atattagatg taaatatatg
     2701 tctatctatt tgaaaaccct taagttgtta agggtaactt tacataaaag tgtgaacaag
     2761 ctggcacaaa ttgtttaatg tttacagcaa aagataacct tcgtggcgca ataaccactc
     2821 ttttcgctga actccgcctg catatccggt catggtgccg tttcggccaa taacccgatg
     2881 gcaaggtacg acgatgctga tgggattcga tccgtttgcc gcaccaacgg cacgcgccgc
     2941 gccagggcgg cccaattgtt cagccagttg gccgtaatgc attacctgtc cgcaggggat
     3001 agtgcgtaga gttttccaga cttcgcgctg aaatggcgtc ccccctgtgg cagtgggaag
     3061 cgtatcaata atgctaagat taccggcaaa ataatcacga agcttgtcgc ttaaaccgcc
     3121 tgggttggtg gcagaaatgc gctcatagcc ttctttgcga taatggatgt ccagcagctg
     3181 caccatgcgt tcgctgtact cttcccattc aaccgcccgc aggcgaaatt gctcatcgca
     3241 aatcacccac agtggaccca gtggcgtggc aattttttct tcaagtaatc tcagcatccg
     3301 ttctctctta agacaaacgt gggtaaatcc ccggacctat cggcagacca acaagatacc
     3361 acgccagcaa catcagcagc cataccacca aaaagataag cggatagggc aagactaacg
     3421 aatagtaagt acccagtttc gcgtctggtt tgtagcgttg caggaatcca agaaacagtg
     3481 gaacaaaagg agataccggc gctaaaggca atacggatga gtcggcaata cgaaagagga
     3541 tttgcgcaaa tgccgggtga aagccaagta gcataaacat tggtacgaaa atgggggcca
     3601 gaatcgacca gattgcggaa ccgctggcaa taaacatgca taagaaagag gaaagcaacg
     3661 ccagaccgac aaacgccggg atgccgctaa gccctgaact ttccagtata tcggtcagcc
     3721 ccacggcgat gaatttc
//
LOCUS       DAZPLI010000262         3730 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZPLI010000262 DAZPLI010000000
VERSION     DAZPLI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.265"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(10..165)
                     /locus_tag="ACHUU3_004195"
     CDS             complement(10..165)
                     /locus_tag="ACHUU3_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR2084105.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(368..775)
                     /locus_tag="ACHUU3_004196"
     CDS             complement(368..775)
                     /locus_tag="ACHUU3_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR2084106.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            852..1079
                     /locus_tag="ACHUU3_004197"
     CDS             852..1079
                     /locus_tag="ACHUU3_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR2084107.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPWKVIHENQA"
     gene            1063..1484
                     /locus_tag="ACHUU3_004198"
                     /pseudo
     CDS             1063..1484
                     /locus_tag="ACHUU3_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
     gene            1562..2355
                     /locus_tag="ACHUU3_004199"
                     /pseudo
     CDS             1562..2355
                     /locus_tag="ACHUU3_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2362..3108
                     /locus_tag="ACHUU3_004200"
     CDS             2362..3108
                     /locus_tag="ACHUU3_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR2084108.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKLGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            3123..3545
                     /locus_tag="ACHUU3_004201"
     CDS             3123..3545
                     /locus_tag="ACHUU3_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR2084109.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            3546..>3730
                     /locus_tag="ACHUU3_004202"
     CDS             3546..>3730
                     /locus_tag="ACHUU3_004202"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084110.1"
                     /translation="MKLAIRLQLPLAFASKERSMEIKPEDELSNIVLFPVKEDDPRNQ
                     VNFLYEPSERPYCHHASV"
BASE COUNT          980 a          798 c         1035 g          917 t
ORIGIN      
        1 tcgttcattt tactcacctg aatgtcttcc caaccaacga cgtgcgccag cttcggtttt
       61 aaacgttttg cttttggtat acgtcatggc ggtgaatgtg ccgtcctggt tggggaacac
      121 gccgtacacc agagattcgt tgttgccaag atcgatagta ttcatgttga ccccatttcc
      181 ccttaacgcc ggggtagcgg aactgtttgc tgagaacacc gtgcggtgtc ttgatgaatg
      241 aaatttagaa taacctaagg tgggcggtca agatttttgt gtagaaaaac ctaagttttt
      301 tgatgtaaaa aacacaagtg tttgaaagtt tgtgcttttt attacagggt gtggagaaaa
      361 aaggggatta tttgtttgcg cttcttttgc gagctttgag tagttcttca aaaagtttgt
      421 tgaaattttc aactcgagca cgcatctctg acaacagggc cttttgctct gactcaggca
      481 gtgcgtcgaa cagttgaagt aactcttttt gatcttctgt cagaatggct ggctgattat
      541 ccgggatcgg ttcgcctggt tgtttatctt catccccaaa aagaagccaa gtcggtgagc
      601 actgaagcgc ttggctcagt gcgaataatc ttttccccgc cggctgtgtt tcatctcttt
      661 cccattgaga aattgttacg tgagccactt tgaccagctt acctaatgcg gcctgagaca
      721 gttttaattt tttacgccta tgtaagaggc gagcaccgaa ggtttcgttt ttcatattag
      781 ggaattctaa tttttcttga cttaggtttc tctacgatct agtttcctta ggaaaatcta
      841 aggagctcga tatgttgaaa attgatgcta tagcgttttt tggcagcaaa acaaagcttg
      901 ccaatgccgc aggagttagg ctggcaagca ttgctgcatg gggggaactg gttcctgaag
      961 gtcgcgcgat gcgcctgcaa gaggcatctg gcggggaact tcagtacgac cccaaagttt
     1021 atgacgaata tcgtaaggca aaacgacctt ggaaggtgat tcatgaaaat caggcatgag
     1081 cacatcgaat cagtgctgtt gccctggcag ccgaaaaagg gcaggcgtgg gtcgctaacg
     1141 caattactga agaatatctg cggcaggggg gcggcgaatt gtctctggta ccaggcaagg
     1201 actggaacaa tcagcagaat atctatcacc gttggttgaa aggtgaaacg aaagcgcaaa
     1261 gggaaaaaat tcagaaactg atccctgcgg ttctggcaat tcttccgcgt gagctgcgtc
     1321 accgactctg tatcttcgat accctggaac gccgtgcatt actggcggcg caggaagcgt
     1381 tgagtacggc aattgatgcg catgatgatg ctgtccaggc cgtttaccgg aaagcacatt
     1441 tcagcggtgg tgggtcgccc ggcgattctg tcgtagtgca ttgattgaaa ttaatcgtgc
     1501 cggattgttt tgttcggtat cagttaaatg taacgctgcg agcgttacaa ggtgaaaaca
     1561 aatggcttca aactggataa agctcgaggt tattacgccg gataagccgg aaatattcag
     1621 gcttgctgag attctgaata ttgatccaga tgccgcatta gggaaggtta ttcgcttctg
     1681 ggcatgggcg gatcaacaaa tgatagacgg taatgcagat tgtaacgctc gcggcgttac
     1741 aaaaagtgca atagatcgca tcacttttat gtctggtttt gctgatgcgt taattcaggt
     1801 tggatggctg gtcgaaaatg acggtgggct ttctctacct aactttgaac gtcataacgg
     1861 aaaaagctct aaaaaaaaac gggcggttac aaacgagcga gtaacaaaaa tacgcgaact
     1921 gaaacgaaaa ggtaacgctg ccagcgttac acaaacggat caaaaagcgt taccagagga
     1981 agaggaagag gaagaggaag aggaagatct aaatactgat ctccccctcg ccaaaaacga
     2041 gcgtctaaaa aattcgagcc ggaggctatt gagctgcccg attggttgcc ggaaacactc
     2101 tggcatgagt gggtccggtt cagacaggca ttgcgaaaac cgattcgaac ggagcagggc
     2161 gctaacgggg cgatacggga actggaaaaa ttccgtcagc agggttttac acctgagcag
     2221 gtgattcgac acagcatcgc caatgaatac cagggcctgt tcgcgccgaa aggtgttcgg
     2281 cctgagacgt tgctccgaca ggttaacacc gtctcgttgc cggacagtgc gatcccgcca
     2341 ggcttcaggg ggtaacggac catgaaaaat attgcgacag gcggcgttct ggaacgcatc
     2401 cgccgactga ccccgccaca tgtaaccgcc ccattcagaa cggtagcgga gtggcgcgag
     2461 tggcaacttg ctgaaggcca gaaacgtagc gaggagatca accgcctgaa tcgccagttg
     2521 cgggtggaaa aaattctgaa tcgctcaggc atccagccgt tgcaccgtaa atgctcgttt
     2581 gcgaattacc aggtgcagaa cgacggccag cgatacgcgt taagccaggc gaaatccatc
     2641 gccgatgaac tgatgaccgg gtgtacaaat tttgcgttca gcggaaaact tggtaccggg
     2701 aagaatcact tagcggcagc tatcgggaat cgcctgctga aagacggtca gacagtgatt
     2761 gtggttaccg tggctgatgt tatgagcgcc ctgcacgcca gctatgacga cgggcagtca
     2821 ggcgaaaaat ttttgcggga gctgtgcgaa gtggatctgc tggttcttga tgaaattggc
     2881 attcagcgcg agacgaaaaa cgagcaggtg gtgctgcacc agattgttga tcgccggaca
     2941 gcgtcgatgc gcagcgtggg gatgctgaca aacctgaact atgaggtgat gaaaacattg
     3001 ctcggcgagc gggtgatgga tcgcatggtc atgaacggcg ggcgctgggt gaattttaac
     3061 tgggagagct ggcgtccgaa tgttagccat tcgagggttg ttaagtagtt tcaggaggat
     3121 ttatggcgaa accttttact cccgaacagc gggaagaact gaagacgcga attgtggaac
     3181 tcgtgcatca ggacggtcgg gtcacgattc ggcagttgtc agatgaaaca ggtatcagtc
     3241 gtgcgtctgt cggtcgctta tgcatagaac tggtcgcaag tggtgatgta tataattctg
     3301 gctacggctt attcccgtct gaacaggctc gcaaggactg gcaaagcgcc cgcaaaaaac
     3361 tctcgagagt aaaggtgagg aaaccggttg ttgttgatcc ggaccttatc tggtcattac
     3421 ctgacggaga aatacgccgc tacgacaggc gcctgaatat aatctgtcgc gagtgccgga
     3481 agagcgaagc tatgcagcgt gtactggctt tctatcaggg taattttcag gaggcgatac
     3541 tgtgaatgaa attagctatc aggcttcaat taccgctggc attcgcatca aaggagagga
     3601 gcatggaaat aaaaccagaa gatgagttaa gcaatatcgt tttatttccg gtaaaagagg
     3661 atgaccctcg taatcaggtt aattttcttt atgagccatc ggaaagacca tattgccatc
     3721 acgcctctgt
//
LOCUS       DAZPLI010000263         3674 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZPLI010000263 DAZPLI010000000
VERSION     DAZPLI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.266"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..95
                     /locus_tag="ACHUU3_004203"
     CDS             <1..95
                     /locus_tag="ACHUU3_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR2084111.1"
                     /translation="RLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            244..1491
                     /locus_tag="ACHUU3_004204"
     CDS             244..1491
                     /locus_tag="ACHUU3_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFR2084112.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(2020..2220)
                     /gene="torI"
                     /locus_tag="ACHUU3_004205"
     CDS             complement(2020..2220)
                     /gene="torI"
                     /locus_tag="ACHUU3_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFR2084113.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2343..2687)
                     /locus_tag="ACHUU3_004206"
     CDS             complement(2343..2687)
                     /locus_tag="ACHUU3_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084114.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2929..3531
                     /locus_tag="ACHUU3_004207"
     CDS             2929..3531
                     /locus_tag="ACHUU3_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084115.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          919 a          740 c          845 g         1170 t
ORIGIN      
        1 cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
       61 tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc cgcttgcagt
      121 aacggcgaaa ggggcaatga cagcgctgcc atgtcgacaa gaactggaat agtgagaagt
      181 aaacggcgaa gtcgctctta tctctaaata ggacgtgaat tttttaacga caggtaggta
      241 attatggcac tgaatattcc attcagaaat gcgtactatc gttttgcatc cagttactca
      301 tttctctttt ttatttcctg gtcgctgtgg tggtcgttat acgctatttg gctgaaagga
      361 catctaggat tgacagggac ggaattaggt acactttatt cggtcaacca gtttaccagc
      421 attctattta tgatgttcta cggcatcgtt caggataaac tcggtctgaa gaaaccgctc
      481 atctggtgta tgagtttcat cctggtcttg accggaccgt ttatgattta cgtttatgaa
      541 ccgttactgc aaagcaattt ttctgtaggt ctaattctgg gagcgctctt ttttggcctg
      601 gggtatctgg cgggatgcgg tttgcttgac agcttcaccg aaaaaatggc gcgaaatttt
      661 catttcgaat atggaacagc gcgcgcctgg ggatcttttg gctatgctat tggcgcgttc
      721 tttgccggca tattttttag tatcagtccc catatcaact tctggttggt ctcgctattt
      781 ggcgctgtat ttatgatgat caacatgtgt tttaaagata aggatcacca gtgcgtagcg
      841 gcggatgcgg gaggggtaaa aaaagaggat tttatcgcag ttttcaagga tcgaaacttc
      901 tgggttttcg tcatatttat tgtggggacg tggtctttct ataacatttt tgatcaacaa
      961 ctttttcctg tcttttatgc aggtttattc gaatcacacg atgtaggaac gcgcctgtat
     1021 ggttatctca actcattcca ggtggtactc gaagcgctat gcatggcgat tattcctttc
     1081 tttgtgaatc gggtagggcc aaaaaatgca ttacttatcg gtgttgtgat tatggcgttg
     1141 cgtatccttt cctgcgcgct gttcgttaac ccctggatta tttcattagt gaagctgtta
     1201 catgccattg aggttccact ttgtgtcata tccgtcttca aatacagcgt ggcaaatttt
     1261 gataagcgcc tgtcgtcgac gatctttctg attggttttc aaattgccag ttcgcttggg
     1321 attgtgctgc tttcaacgcc gactgggata ctctttgacc acgcaggcta ccagacagtt
     1381 ttcttcgcaa tttcgggtat tgtctgccta atgttgctat ttggcatttt cttcctgagt
     1441 aaaaaacgcg agcaaatagt tatggaaacg cctgtacctt cagcaatata gacgtaaact
     1501 tttccggttg ttgtcgatat ctccctatcc ctcaaccgga aaataataat actaaagtgc
     1561 ttagcccggc taataatcac ctaatccaaa cgcctcattc atgttctggt acagtcgctc
     1621 aaatgtactt cagatgcgcg gttcgctgat ttccaggaca ttgtcgtcat tcagcgacct
     1681 gtcccgtgta tcacggtcct gcgaattcat caaggaatgc attgcggagt gaagtatcga
     1741 gtcacgccat atttcgctat caggattctg tgtgatggtt acatcgcccg gcccagggct
     1801 gtttagtcat cagcgctttc tgacagtgct gagatttcaa cctgttgcag taaaaatgag
     1861 tagatataag gcaagtgtgc tgccaaaccc atcttttacg gggtgaaggt agatttcgtt
     1921 tgaagggtat ctggtgtccc ctgcagacat ctacttgagg cggcagggga ttgattggaa
     1981 tgctgttttt tagatttgaa aaatatttta cccgctattt tacccattgg cgcggcttaa
     2041 gagcttattt ttgaattcac aatggtcacg atataaccat cttgctcgtc cgtggataac
     2101 tttggcttta ggcaggtcgc cggacttaat ccggtcatag atgaaggtct taccgaagcc
     2161 agtatcagcc atgatgaatt tcaaatcaac cagtgaatca ggttgtagtt cgtgttgcat
     2221 gagtgctatc tccgaatatg gaatcgaacc tgcaaatcag gcaataaaaa gccgcattga
     2281 tgcagcaatg gtaggtctgg atatcttgag aaatgaacag gcctcatcga gtgtgaggcg
     2341 ggttagtcct tgcgtagctc gctgattctt ctgtaagtct ctggtgcttt gttcccgtac
     2401 gtcttcattt cagacttcaa cagagcaacg agtgaatccc attcgttgag gattcctttg
     2461 aatgccggaa cgcgctttgc aaccttgtcg aatgaatctc tgatttctgg aatctgctca
     2521 acaagtgcaa cgcatcgccg aaagtctgct gcgtcatggg gagcgccgaa gtgatgacca
     2581 tagatattct ttttcaggcc acatgcgatt gaggcaagag ttgcgctact gatgccgaca
     2641 tcgccagttg attgccattt caaaaccttc atagccaaat ctgacatttc ttgtctccat
     2701 aaaacaaaac tcgccgtagc gagctcagat aaaagaaatc cccgtcagtg cgaggatgct
     2761 gttcattgct gctatacact tttttgctct caacgtaagc ggtagcccat tctgttgggt
     2821 tggtcagttg cttttaggaa atgctcttta ccccttaaac gtcggctgaa agagctaaaa
     2881 tccatgcaaa aaatttacgc aattttgtgt attattgtgc agtaagtaat gagctatttt
     2941 ctgtgcaaaa aatggatgat aaatttgtcc ggggcaggaa aaattttatg ggcgctaaac
     3001 atgaaaaaag attcgtatcc ttatttgatt tgcatgacag tttcagggct gatctttatt
     3061 ttccttttct tctggtggcg ggcagatatc tacagggtca cgtttcttaa tcagagtata
     3121 tcccactatt acattctgtt tagcatggga atagcttttc tgttatctct gttttgggtt
     3181 aagaagggga tagtaaaaca aagcggctgg aagagtctgt cagcatacct taaggtttat
     3241 gcagggatat gcatattttc tggatttttt ctgattatac cccttacaac actaacttat
     3301 tttttgcctg gagagacatc gtcttatgtt gcaccgtatc ggtatacttc cggtagttca
     3361 aaaagttgtt ctggagctga ggtggatgac cccgatctac atgagaatat tcgcatttgc
     3421 tatccgtatg gcaattatga gtacgataat attatctatg ttgaaaagaa aattaataca
     3481 ttaggtgcgg tagtgacata tgcacagacc gcgcgtgatg atactgaatg agatagtata
     3541 tagcgggcaa gttttagtta atttatcgag gtaatataat ttacctcgac tcgtttattc
     3601 tggtatgaat atttcgcttt acgatcgatt tttatctgat gatatcatgc ggttttcata
     3661 tactgactta ctgt
//
LOCUS       DAZPLI010000264         3627 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZPLI010000264 DAZPLI010000000
VERSION     DAZPLI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.267"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(89..652)
                     /locus_tag="ACHUU3_004208"
     CDS             complement(89..652)
                     /locus_tag="ACHUU3_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR2084116.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(756..974)
                     /locus_tag="ACHUU3_004209"
     CDS             complement(756..974)
                     /locus_tag="ACHUU3_004209"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084117.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1133..1519)
                     /locus_tag="ACHUU3_004210"
     CDS             complement(1133..1519)
                     /locus_tag="ACHUU3_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR2084118.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1509..1880)
                     /locus_tag="ACHUU3_004211"
     CDS             complement(1509..1880)
                     /locus_tag="ACHUU3_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR2084119.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(1880..2137)
                     /locus_tag="ACHUU3_004212"
     CDS             complement(1880..2137)
                     /locus_tag="ACHUU3_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084120.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2134..3525)
                     /locus_tag="ACHUU3_004213"
     CDS             complement(2134..3525)
                     /locus_tag="ACHUU3_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR2084121.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            complement(3522..>3627)
                     /locus_tag="ACHUU3_004214"
     CDS             complement(3522..>3627)
                     /locus_tag="ACHUU3_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR2084122.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT          850 a         1013 c          811 g          953 t
ORIGIN      
        1 catcggcggg tttaagctgt gtggcgtagt aaccactctt aacaggatat tcaacttttt
       61 acgatcgtaa agttttcgga aaaaattttt aaaaccgcat caaccgctcc accagttgct
      121 ctttacgggc aacgatccac ccgtgttgct ccagataaaa tttaaaccgt tccagagtac
      181 ataccatcgc atcggcgggt accttttccg tgaactcaac ctgaccgtgt ttatcgaagt
      241 ggctcagtaa tgcgcatcca tcattttcga tagatgtgtt ttgtgccgct ggtggctgtt
      301 tctggttgaa ataacagtct tccagttttt cgaacacttc ccacgcctga tcggtttcga
      361 gcattttggc gtgacgggct gcgccacgtt ctgtccagag gatgagggag cgaacgttac
      421 gggcaatttt cacagaaact cttaaagagt tttcgttctt caactctcgt aacgcatcac
      481 cttctatcag gaaaaaatgt ttaccttcta taaaccgttc tttgtttcga ttgaaattgt
      541 ttgtcaaacg ctggcgctct gttccgtaca aatcagccag cagctctgtt gtcatgacag
      601 gaagatggtt atgcgtaagt gacgggagtt tttcaataga cgtgcgattc atagacatgt
      661 cctttcgaat attttgattt accccttttg agagggtgac cgggcgctca aaaccgtcga
      721 aagtcggcgg gcgtatttcc attgctggta ttgtattagc cgcacacccg gtcataaacc
      781 aagaattctg gacacaaaaa aaccacatgg ctatcgggtg tggataccgc tttcgaggtg
      841 ttttgagcac cgcggggaaa tgtaaactgc aaacctgaaa ctgtcaacac cgtcaggttc
      901 caatcatcgg gtggtgagac gcacagggtt ggaactaccg ggaaaccgac cggcgagctt
      961 ttcagctccc ccatgcgccc caccataatt cagatgtgcg cgtgcatacg acaataaaaa
     1021 acacgctcgc ggcgtgtgtc tgtcgcggtc tctatccggg gttccaatcc cgacgccaga
     1081 ttttgctggc gcgtgaggaa tatagccccg gacaatgtgt ctggtcaagc acctacataa
     1141 ttcgttctac gtatctgtcc atctccagcc ggatatcaag catcatcaac atgccatcaa
     1201 taaccccttc cgctttctgc aggcgcttgc caatacaggt atccgaacac ccatgtttcc
     1261 gtgccagact cataaaagtc attccaccta cgtaataatc caccagcaaa tcgtgcaaat
     1321 cctgattttt cttgttcaac cgggccatac agccacaaat tatcattgca tcatcatcag
     1381 aacactgagg gcgtgatttc actttcgacg gaattaaccc tttaaaacca gcagcgatcg
     1441 acgcccagga tacatcttca tgagtgtttg cagcccaagc cccccaccgt tccataacct
     1501 gctgaatatc acgcgccatc gttatcacct gtgatttcat aaatcttcac gcccagtcgc
     1561 ccaccaggaa cgagctgacc gcgcacaata ttgatttcat caaactgctc gtcgtctatg
     1621 agtagtccgg catgcgtcag cgcatccagt ggtgctttca ggatattgtc caggtcacga
     1681 cggcgcttat ccggtggctc tgcaataatc tttatcgcca gccttccgga caggtttaat
     1741 ttcagccgct gctggcgaac aattagcgtc acatcacggc gataacgctc accaactttt
     1801 gatacaaaat atgtgctgcc acgacgtcgc cagtaagtat tcaccgtcgg cggataaggt
     1861 aaaataaact catggcgcat cagcgcagca cctcctgcac cattttttca aactttccaa
     1921 ctttggtttc cagctctgcc acacaatcaa cgagctcatc caccgctttt tgtgcgcgat
     1981 gtttcgcctg catcagttcc ctgagcgctg gcaccatatc ccgacgaatg gcatcttttg
     2041 ttacacctgt tttttccagt tgttccgcct gtcgcaacat ttcctgcgcg tgtttacgca
     2101 attgttcaga ggtaaaagtc attgtctggt tgttcaaaag aaacgctcca tcttactgct
     2161 gtcggtacgt gtattgctgt atctgcgcgg ctggggctgc cgcattgggg tggaaagaac
     2221 ctgtgcgctt tcctggtcca caggcagaaa atgtccgtta taaaaacgcc ggtaaatcgt
     2281 ccccagagaa ccgttacgtt gtttcgtgat attaatttct gctatgcctg tagcctgtgt
     2341 atccgggttg tacacttcat ccctgtaaag catcagaatg atgtctgcat ccgcctctat
     2401 ttctccggaa tttttcaggt ctgagttcat gggacgttta ttgggtctgg actccacacc
     2461 gcgggagagc tggctcagcg caatcaacgg aaaaccaccg gattttgcca ggcctttaag
     2521 cccctttgag atttcaccca cggcaaggtc atgacgcccc gtggttcggg tttttatcag
     2581 cccgagataa tcaaccacca ccagcgccgt ttccggatgt ttaatcagat ggtgtttcgt
     2641 tgttgcgcat atctcatcaa tggtcaggtt cgcctggtcc accatccaga tattgcgccc
     2701 ggtcatccgc cccacccctt gtgagaaacg cgcccagtct tcatcttcaa agtgagccac
     2761 agatttcagg cgtgatactg gcatccctcc agccgcagac accatacgtt caccaatctg
     2821 gatgttcgcc atctccatgg tgaacagaag cacaccatgc ccctgctcag tcaccttgtc
     2881 gatgatgtcc agcgcaagtt cggttttccc catcgaagga cgggcggcaa tgaataccag
     2941 gtctccgggc tccataccgc ctgtttttgc gtccagttca tcaataccgg tcatcaacgt
     3001 cctggatttc tccagcccct gattccggca ttcaacacgc tcaaccactt ccggaagcac
     3061 atcatcaata tgtaccggct gaatgacgcc ctttcctgtc gacagtgtga ccatcatgtt
     3121 ctgcgcatcc ttcagggcat cttcagctgc ttcacaggta tgcgcatcac gtaatttctg
     3181 cagcgcctca ttcagtgttt tttctgcatc gcgcagtgcg gcattgcgcc gcaacgctgc
     3241 aacatagtgc tccagtgaag acttcaccca ggttttacgc ccggtatcag taatcaccgg
     3301 ggcaagttcc ggcatctcat tacacaacag cacggggtca atcactcctg aaacacgggc
     3361 ctgtctgcag atgcctgtgt agatatcccg atacgctcgt acagaaaaaa tgtccgccgg
     3421 tagtgtgatc agaatatcca tcacttcatg atctgcccca cgcagaaaga atgcgccaat
     3481 gacagcgcct tccaggtcat cattacgcca gactggtgtt gtcatgcagc cacacctctg
     3541 atacaagaac ggtagctggg ccagttgaac gacaaccagt tgcgcccccc gtctgtgatc
     3601 ctgtcggcaa tgcgggggct gatgaac
//
LOCUS       DAZPLI010000265         3618 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZPLI010000265 DAZPLI010000000
VERSION     DAZPLI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.268"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            414..695
                     /gene="drpB"
                     /locus_tag="ACHUU3_004215"
     CDS             414..695
                     /gene="drpB"
                     /locus_tag="ACHUU3_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR2084123.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
     gene            735..1391
                     /locus_tag="ACHUU3_004216"
     CDS             735..1391
                     /locus_tag="ACHUU3_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFR2084124.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            1458..2876
                     /gene="dcm"
                     /locus_tag="ACHUU3_004217"
     CDS             1458..2876
                     /gene="dcm"
                     /locus_tag="ACHUU3_004217"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR2084125.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2857..3327
                     /gene="vsr"
                     /locus_tag="ACHUU3_004218"
     CDS             2857..3327
                     /gene="vsr"
                     /locus_tag="ACHUU3_004218"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR2084126.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3316..>3618)
                     /locus_tag="ACHUU3_004219"
     CDS             complement(3316..>3618)
                     /locus_tag="ACHUU3_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFR2084127.1"
                     /translation="PSLSGFLAVGYLALFGSIIAINAYMYLIRNVSPALATSYAYVNP
                     VVAVLLGTGLGGETLSKIEWLALGVIVFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
BASE COUNT          916 a          852 c         1004 g          846 t
ORIGIN      
        1 aatggaaaga tagggagtat caatgattgt tgcgccaaac ggcaggcgtg atcacgcgca
       61 cattaatgaa ggtataaaaa aagccggcat catgccggcc caaaaaatat aaacaagacg
      121 ataaaaggct aataacgggg ttatcatgac acagaaatca aaaaaggtat aattgtattc
      181 agtgctaata tattgtttgg gtgaattaat aaaactatta ccaaggatat aaataaatct
      241 ttattgctgt ttttccagta attattgccg ttgcgcaaaa tttatctatt tgttcaaaaa
      301 atgattgagt cttgactggc tcatccaatg tggaaaaatg tgacttttat cacataatag
      361 tactaagtct gaattttccg ggttatctca aaatggaata cggttcgaca aagatggaag
      421 agagactctc tcgcagccct ggcggcaaat tggctttgtg ggctttctat acatggtgtg
      481 gctatttcgt ctgggcaatg gcgcgttaca tctgggtgat gagccggatc cctgatgccc
      541 cggtgtcggg atttgaaagc gatttaggct ctaccgcggg aaagtggtta ggggcactgg
      601 tcggattttt atttatggct ctggtcgggg cattgttagg cagcattgcc tggtacaccc
      661 gacctcgtcc cgcacgtagc agacgctatg aataaaaact gctaaggtgg cgactctttg
      721 gcaacggagc gcgtatggac ttacaacact ggcaggcaca gttcgaaaac tggttgaaaa
      781 accatcatca gcaccaggac gcggcacaga agctggcggc agatgacgat gtcgatatgc
      841 tggtgatttt aaccgcctgt tattttcacg atattgtgag cctggcgaaa aatcatccgc
      901 aaaggcagcg ttcttcaatc ctggcggcag aagagacgcg tcgcctgctc cgtgaagagt
      961 tcgagcaatt tccggcggag aaaatcgagg ccgtttgtca tgccattgcc gctcacagtt
     1021 tcagcgcgca aatcgccccc ttaacaacgg aagctaaaat cgtccaggat gcggatcggc
     1081 tggaagccct gggggcgatt ggcctggcgc gcgtgtttgc cgtttcaggg gcattgggcg
     1141 tggcgctgtt tgatggtgaa gatccgttcg cacagcatcg cccgcttgat gataaacgct
     1201 acgcgctgga tcatttccag actaagttgc tgaagctacc gcaaaccatg caaaccgcaa
     1261 gaggcaagca gctggcgcag cacaacgcgc attttttagt cgagtttatg gcgaagctca
     1321 gtgccgaact ggcgggggag aatgaaggtg tcgatcacaa ggtgatagat gcgttttcac
     1381 ccgcgggata agcgcgtggc cctaaatggc tgtaattatg ttaacctgtc ggccatctca
     1441 gatggccggt taaatctatg caggaaaata tatcagtaac cgattcatac agcaccggga
     1501 atgccgcaca ggcaatgctg gagaaactgc tgcaaattta tgatgttaaa acgctggtgg
     1561 cgcagcttaa tggtgtgggt gagaatcact ggagcgcggc aattttaaaa cgtgcgctgg
     1621 cgaatgactc ggcatggcac cgtttaagtg agaaagagtt cgcccatctg caaacgttgt
     1681 tacccaaacc atcggcacat catccgcatt atgcgtttcg ctttatcgat ctatttgccg
     1741 gaattggcgg catccgtcgc ggttttgaat cgattggcgg acaatgcgtg tttaccagcg
     1801 aatggaacaa acatgcggta cgcacttata aagccaacca ttattgcgat ccggcgacgc
     1861 atcattttaa tgaagatatc cgcgacatca ccctcagcca taaagaaggc gtgagtgatg
     1921 aggcggcggc ggaacatatt cgtcaacaca ttcctgaaca cgatgtttta ctggccggtt
     1981 tcccttgtca gccattttcg ctggctggcg tatcgaaaaa gaactcgctc gggcgggcgc
     2041 acggttttgc ctgcgatacc cagggcacgc tgttttttga tgtggtacgc attatcgacg
     2101 cgcgtcgtcc ggcgatgttt gtgctcgaaa acgtcaaaaa cctgaaaagt cacgaccagg
     2161 gtaaaacgtt ccgcatcatc atgcagacgc tggacgaact gggctatgac gtggctgatg
     2221 cagaagataa cgggccggac gatccgaaaa tcatcgatgg taaacatttt ctgccgcagc
     2281 accgtgaacg catcgtgctg gtgggttttc gtcgcgatct taatctgaaa gccgatttta
     2341 ctctgcgtga tatcagcgaa tgtttccctg cacagcgagt gacgctggcg cagctgttgg
     2401 acccgatggt cgaggcgaaa tatatcctga cgccggtgct gtggaagtac ctctatcgat
     2461 atgcgaaaaa acatcaggcg cgcggtaacg gcttcggtta tggaatggtt tatccgaaca
     2521 atccgcaaag cgtcacgcgt acgctgtctg cgcgttatta caaagatggc gcggaaattt
     2581 taatcgatcg cggctgggat atggccacgg gtgagaaaga ttttgacgat ccgctgaatc
     2641 agcaacatcg tccacgtcgg ttaacgcctc gggaatgcgc gcgcttaatg ggttttgaag
     2701 cgccgggaga agcgaaattc cgcattccgg tttcggacac tcaggcctat cgccagttcg
     2761 gtaactcggt ggtcgtgccg gtctttgccg cggtggcaaa actgcttgag ccaaaaatca
     2821 aacaggcggt ggcgttgcgt cagcaagagg cacaacatgg ccgacgttca cgataaggcc
     2881 actcgcagca aaaatatgcg cgcgattgcc acgcgtgata cggcgattga gaagcgcctc
     2941 gccagtctgt taaccgggca gggcctggcg tttcgcgttc aggacgccag tctgcccgga
     3001 agcccggatt ttgtcgttga tgaatatcgc tgcgtgatat ttacccatgg ctgcttctgg
     3061 catcatcatc actgctatct gtttaaagtg cctgcgactc gaaccgagtt ctggctggag
     3121 aagataggta aaaatgttga acgcgatcgc cgcgatatca gtcgtttgca ggaactcggc
     3181 tggcgcgtac tgattgtctg ggagtgtgca ttacgtgggc gcgagaagct gacggatgaa
     3241 gcgcttaccg agcgtctgga agagtggatc tgcggcgaag gtgccagcgc gcagatcgac
     3301 acgcagggga ttcatttact cgcttgatgc gtcctgaata actggcgcaa ctacgggttt
     3361 tgccgggaag agatattttc ccaacgtgac cagtaccacc gcgaagacaa ttacgccgag
     3421 cgccagccat tcaatcttcg acagcgtctc tccgcccagt cccgtaccca gcaagaccgc
     3481 gaccaccggg ttaacgtagg cgtagctggt ggcgagagcc ggactgacat tacggattaa
     3541 atacatataa gcgttgatgg cgataatcga accaaacagc gccagatagc cgaccgcaag
     3601 gaagcctgaa agggaagg
//
LOCUS       DAZPLI010000266         3571 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZPLI010000266 DAZPLI010000000
VERSION     DAZPLI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.269"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..216
                     /locus_tag="ACHUU3_004220"
     CDS             <1..216
                     /locus_tag="ACHUU3_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR2084128.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTRVQGTVVMFSASGKK"
     gene            complement(161..298)
                     /locus_tag="ACHUU3_004221"
     CDS             complement(161..298)
                     /locus_tag="ACHUU3_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFR2084129.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(271..369)
                     /locus_tag="ACHUU3_004222"
                     /pseudo
     CDS             complement(271..369)
                     /locus_tag="ACHUU3_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(358..597)
                     /locus_tag="ACHUU3_004223"
                     /pseudo
     CDS             complement(358..597)
                     /locus_tag="ACHUU3_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905063.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            696..1586
                     /locus_tag="ACHUU3_004224"
                     /pseudo
     CDS             696..1586
                     /locus_tag="ACHUU3_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            1589..2122
                     /locus_tag="ACHUU3_004225"
     CDS             1589..2122
                     /locus_tag="ACHUU3_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR2084130.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(2151..2678)
                     /locus_tag="ACHUU3_004226"
     CDS             complement(2151..2678)
                     /locus_tag="ACHUU3_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR2084131.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(2680..>3571)
                     /locus_tag="ACHUU3_004227"
     CDS             complement(2680..>3571)
                     /locus_tag="ACHUU3_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR2084132.1"
                     /translation="ETVNKAGNAVQRSGDKMTGELKIGTVNALRIFNDTFGLIFRRSE
                     DFLHFIPTAEGQGENGNIGPLRPFAINLRTGAISVSHGAKIKGGLAIGATDNALGENS
                     IVLGDNDTGFRQDGDGIISFYSNGSRIGHIDELGLHLYKDIESNGSNFRLKSNYRHHI
                     TFANEDGRIRMFLWKDNGGDGVHINNGSDGGGDFIFKTDGGFALGSGAQVASSGDIYG
                     SVWENNWLSTWLHNHVVRDIRLGSIEYKNVWRDYGFGDASGYVLTAAINSNADDIVDT
                     VARRPIQKLIGGIWYNVGSV"
BASE COUNT         1007 a          809 c          755 g         1000 t
ORIGIN      
        1 gatggaactc tatacggagc cacaattctt ggcgatggag ttgtgcacaa tagcttcggt
       61 caaaaactga tgcgcattta caatcagaaa ggcatctttt caaacacaaa agattccgaa
      121 gaaggcttaa cacatatact ctcagagcat ttcgagaatg ttaaaaccag ggttcaaggt
      181 actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc acgttcttgc
      241 ttaagacgtg ctgcggcata atcccaatga ttactccctg acagggttcg taggccactc
      301 aatatcaggt gcagttgatg tatcaacacg gttcagcaac acccgatact ttttctaggc
      361 ttccaccacc agcacgacaa gatgccgcat acagtgaccc agtcagtcca gttttcagac
      421 aaccagtgcg tcaccttttt gaaggcgctt taaagcacgt tttaatccag gtcggcctgt
      481 ccttgttccg cttaatttat cttcaaatat ttgttcatat cctgcacaaa caagagcgtt
      541 tcgttgcagg tctgtattct ggtcatttgt tgataccctt acatagccaa tcagcacgct
      601 gaatctcccg tccaaaagca caaatcatgc catgcaggcc agaaaccgcc attatctaaa
      661 acctcggttt acgagaactc ggcacaagcg gggagaaaat accgttactc agtacagcga
      721 atacctggac taatcgacaa acattcagcg gtggcctttc tggtgaacta tccggcaatg
      781 cttctacagc tgcaaaatta aaaactgcca ggaaaataag caatgtggct tttgatggtt
      841 cctccgatat cacattaaaa gcaagtcatg ttggtgcgtt tgccttaggg aaaacaggaa
      901 gcaccgttgc gaatgataaa gcagttggat ggaactggag tagcggagcc tataacgcaa
      961 ctattagtgg tgcatcaacg ttaattattc atttttatat gggagaagga agttgtcctg
     1021 cagctcagtt tcggattaat tataaaaatg gcggtatttt ttatcgttca gcccgtgatg
     1081 gttatggttt tgaagccgac tggtccgaat tttacaccac cagaaaacct tcagcaggag
     1141 atgttggtgc actgccgtta tctggtggtc aactgaatgg tgcactgggt atcggaacat
     1201 ccagtgctct tggcggtaat tcgattgtat tgggtgataa tgacacgggc tttaaacaaa
     1261 aaggcgatgg taatctggat gtttatgcta ataacgtcca tgttatgcgc tttgtctccg
     1321 gaagcattca aagtaataaa accataaata ttacgggacg tgttaatccc tcggattacg
     1381 gtaactttga ttcccgctat gtgagagatg tcagacttgg cacacgagtt gtttagacca
     1441 tgaagaaagg cgtgatgtat gaaaaatcag gccatgtaat cacggggctt ggtattatcg
     1501 gtgaagtcga tggtgatgat ccggcagtat tcagaccaat acaaaagtta attaacggaa
     1561 catggtataa cgtatcgcag gtataatcat gcagcattta aaaaacattg tcgcaggtaa
     1621 tccaaaaacc gttgaacaat accagctaac aaagaatttt gatgttatct ggttatggtc
     1681 cgaagatgga aaaaattggc atgaggaatt aaaaaacttt caggaggata caataaaact
     1741 ggcttacaca gttgagggaa taattattgc tatagacaag gatgtatcag caattaatcc
     1801 agaaggttta agtgtcgttg agttgcctga tattacagca aatcgccggg ctgatatttc
     1861 gggcaactgg atgttcaaag atggtgcggt gataaagcga acttataccg aggaagagca
     1921 aaggcaacaa gcggaaaatg aaaagcagag cctgctgcag ctcgtcaggg ataaaaccca
     1981 gctgtgggac tctcagctac agctgggcat catttccgat gagaataaac aaaaattaac
     2041 cgagtggatg ctctatgcgc agaaggttga atccacagac acctccagcc tgccagtaac
     2101 gtttcccgaa caaccagaat gagagaaggc ccgctatcgg gccttaattt ttattcaggc
     2161 ttttgtggcc attcaggatt tgccgtatcc acacggctga ccagaacact gtagcgttcc
     2221 catgcttcca gtcgtgtgcg ttcctcgtct gttgccatat tcagcctgac agcgcgttcc
     2281 agcggctgga tgactgattc agcttcggaa agcaatgcgg ccttttgtga ttcggcctgt
     2341 tgttgcagtt cctgttctgt atattccctc tttactactt taccatccag aaacatccat
     2401 tttccgttat tgtctgcgtg acgatttgcc gtaatatcag gaacttcaac tatgcttagc
     2461 ccttcaggat taatagttga aacgtcttta ctgacagcaa cgatgatgtt gttctcatcg
     2521 taagcaattt ttatggtgtc tggctgaaaa ttcttttgtt cctcatacca gtttttgcca
     2581 tcttcgcaaa agagccatac cacgcccgct tttttagtta gctggtattg ttccggtgtt
     2641 ttcggattcc cggccttaat atttttcagg tgcatcatct taaacactcc ccacgttgta
     2701 ccatattccc ccaatcaatt tctgaattgg ccttctggca acagtgtcga caatatcatc
     2761 cgcattgctg ttaattgcgg ctgttaaaac ataacctgac gcatcgccaa agccgtagtc
     2821 gcgccatacg tttttatatt caatgctgcc aagacgaata tcccgaacga catgattatg
     2881 cagccatgtg cttaaccagt tgttttccca cactgaacca taaatatcgc cactagacgc
     2941 aacttgcgca ccacttccca gcgcaaatcc cccatctgtt ttaaaaatga aatcaccacc
     3001 accatctgaa ccgttattaa tatgaacacc atcaccaccg ttatctttcc acaaaaacat
     3061 acgaattctt ccgtcttcgt tggcgaatgt aatgtggtgg cggtaattac tttttaatct
     3121 aaaattgctg ccattagatt caatatcttt ataaagatgt aatcctaact catcaatatg
     3181 tccgatgcgc gaaccatttg aatagaagct aataatacca tctccatctt gcctaaatcc
     3241 ggtgtcgtta tctcccagaa caatggagtt ttcacccagt gcgttatcag tagctccaat
     3301 agctagacct cctttaattt tggccccgtg gctgacagat atagcacctg ttctcagatt
     3361 tatagcgaat ggccttaatg ggccgatatt accgttttcg ccttgtcctt cagccgtcgg
     3421 aatgaaatga agaaaatctt ctgaacgacg gaaaattaga ccgaaggtat cattgaaaat
     3481 tcgcagcgca ttcaccgtgc caattttcag ttctccggtc attttatcgc cggaacgctg
     3541 aacggcgtta ccagccttgt ttaccgtttc c
//
LOCUS       DAZPLI010000267         3543 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZPLI010000267 DAZPLI010000000
VERSION     DAZPLI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.270"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..140)
                     /locus_tag="ACHUU3_004228"
     CDS             complement(<1..140)
                     /locus_tag="ACHUU3_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR2084133.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAV"
     gene            complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHUU3_004229"
     CDS             complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHUU3_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR2084134.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(696..2150)
                     /gene="xylB"
                     /locus_tag="ACHUU3_004230"
     CDS             complement(696..2150)
                     /gene="xylB"
                     /locus_tag="ACHUU3_004230"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFR2084135.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(2222..>3543)
                     /gene="xylA"
                     /locus_tag="ACHUU3_004231"
     CDS             complement(2222..>3543)
                     /gene="xylA"
                     /locus_tag="ACHUU3_004231"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFR2084136.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          925 a          982 c          783 g          853 t
ORIGIN      
        1 acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag ccctaacaga
       61 taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc cggtgaatag
      121 gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta agagctttat
      181 gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa aaaggcgatg
      241 gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat aaacaagaga
      301 ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc cagttgcccg
      361 agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa cacgccaaga
      421 aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag ataaaccagc
      481 gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac gatctccata
      541 tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaaaaat gccattaaca
      601 aatgattttc agaataaatt catactaaat attaattaat tagtgagata tatagatgtg
      661 aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg cagaagttgc
      721 tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg ctgcgcatct
      781 ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga tttctctgga
      841 ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc cccccccgta
      901 cggtaatcga gctgctgacc gctgatatcc gccagcatct gacgccagta ctcactacgc
      961 gccccgccac caatcaacgt aacactttgc ggtttaatgc cgcaggcatg cacgacatcc
     1021 atgccatctg ccagcgcata acccacgcct tccagcactg ctcgcgccag ttcattgggg
     1081 ccatgttgat gagtcaaacc aaagaaaacc cccttcgcct ggggattatt gtgtggtgta
     1141 cgctcgccgg aaagataagg cagaaaccaa actggctcga cactttcatc agcctgttga
     1201 gctgcagcga ttaaagctgg gacattgcac aggccggtta atttcgcggc ccaatccaga
     1261 cacgatgctg cactcagcat tacagacatt aaatgccaac gctgcggtag cgcatggcaa
     1321 aagctgtgta cagcgctttc tggcttgctt aagaaccctt cgctgacagc aaaatagacc
     1381 cccgacgtcc ccagcgataa cattgcctga ttagcatcaa ccattcccac accaactgca
     1441 ccagctgcat tgtcgccacc gcctgcgaca actggcaccg tcgccatacc ccacgctttc
     1501 gcaacttcag gtaacaaagc accagtaatt tcgctgcctt cgtataatgc gggcatctgg
     1561 tcacgagata agtcgcaagc ctgcagcatg acgtcactcc agtcacgctt tgcgacatcc
     1621 agccacatgg tgccagctgc gtcagacata tcgctggcaa actcccccgt catacgcaga
     1681 cgcaagtaat ctttcggtaa taatactttg tcgatttgac ggaatatctc cggctcatgc
     1741 cgctgaaccc atagcaattt aggcgcagta aatccgggca tcatcaggtt gccggtaatc
     1801 actcgtgatt gcggaactct cgcttccagc aaagtgcact cttgcgcaca gcgcccgtcg
     1861 ttccacaaaa tggcagggcg caatacccgt tgttgagcat ccagtaaggt tgctccatgc
     1921 atctggccgg caatacccaa tgctttaacg tcctgcagag aatgctgatc gcccagagct
     1981 ttcattgcgc gatcagttgc ctgccaccac tgttccgggt cttgttccga ccagagtgga
     2041 tgcgggcgcg aaacggtcag cttttccgtt tgcgaatcaa ccacctcacc ctgctcgttg
     2101 agcaaaataa cttttacgcc cgaggtgcca agatctatcc cgatatacat atcgatcgtt
     2161 ccttaaaaaa atgcccggta tcgctaccga taaccgggcc aacggactgc acagttagcc
     2221 gttatttgtc gaacagataa tgatttacca gattttccag ttgctcctgg cgaccactct
     2281 gatgcaccgg agacaaatta tgttcctgag catatttggc taaatctgcc agtgacattt
     2341 ggcctttcag gatttgctgg cccaattcgc tattccagcc ggaataacgc tgcgcgatgc
     2401 gtttatccag ctcgccatct tcaatcatgc gcgctgcaat tttcagcgcc agtgccatcg
     2461 tatccatcgc gccgatatga ccgtaaaaca gatcatattt atcagtactt tgacgacgta
     2521 ctttggcatc gaagttcaga ccaccggtgg tgaaaccgcc tgctttgaga atttcataca
     2581 tcaccagcgc attctcttcc acactgttcg ggaactggtc ggtgtcccag cccagttgcg
     2641 catcgccacg gttggcgtcg acagaaccga acaggccaag cgcaatggcg gtggctattt
     2701 catgatggaa agagtgacct gccagcgtcg cgtggttagc ttcaatgttc agtttaatct
     2761 ctttttccag accaaactgt ttcaggaagc catagaccgt cgcggcatcg taatcatatt
     2821 gatgtttagt cggttcttgc ggtttcggtt cgataagcaa cgtgccctgg aagccgattt
     2881 tatgtttatg ctcaaccacc atctgcataa agcggcccag ttgttcacgc tcctgacgca
     2941 agtcggtatt taacagcgtt tcgtaacctt cacgaccacc ccacaggaca tagttttcac
     3001 cgcccaattt atgggttgct tccatcgctg taacaacttg cgttgccgcc cagctgaaga
     3061 cttcaggatc tgggttcgtc gccgcacccg cgccatagcg agggtttgta aagcagttag
     3121 cggttcccca cagcagcttc acgccgctct cttcttgctt ggctgccagg acatcaacca
     3181 tttgcgcaaa attattgatg tactctttta acgacgcgcc ctcaggggaa acatccacat
     3241 cgtggaagca ataaaatggc acatgtaact tgtggaaaaa ctcaaatgcg acatctgctt
     3301 tacgcttcgc caacgccagt gcctcaccag gctgctgcca cggacgatta aacgccccca
     3361 caccaaacat gtccgccccg ttccagcaga aggtgtgcca gtagcaggcg gcaaaacgca
     3421 agtgctcttc catacgctta cccaacacca gttcgtcggg attgtagtga cggaatgcta
     3481 acgggtttga ggattttgag ccttcataac gaacgcgatc gagctggtca aaataggctt
     3541 gca
//
LOCUS       DAZPLI010000268         3518 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZPLI010000268 DAZPLI010000000
VERSION     DAZPLI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.271"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..363
                     /gene="trbI"
                     /locus_tag="ACHUU3_004232"
     CDS             <1..363
                     /gene="trbI"
                     /locus_tag="ACHUU3_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFR2084137.1"
                     /translation="DVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNAPMTAAFNM
                     KQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVSPAVVQGAP
                     DITREIQQDIARRMRAEP"
     gene            360..671
                     /locus_tag="ACHUU3_004233"
                     /pseudo
     CDS             360..671
                     /locus_tag="ACHUU3_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
     gene            666..1664
                     /locus_tag="ACHUU3_004234"
                     /pseudo
     CDS             666..1664
                     /locus_tag="ACHUU3_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
     gene            1684..2431
                     /gene="traX"
                     /locus_tag="ACHUU3_004235"
                     /pseudo
     CDS             1684..2431
                     /gene="traX"
                     /locus_tag="ACHUU3_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            2486..3046
                     /locus_tag="ACHUU3_004236"
     CDS             2486..3046
                     /locus_tag="ACHUU3_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR2084138.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            3176..3388
                     /locus_tag="ACHUU3_004237"
     CDS             3176..3388
                     /locus_tag="ACHUU3_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR2084139.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT          812 a          884 c         1153 g          669 t
ORIGIN      
        1 gatgttacag ccccccgacg cagccactgg tggtggacgg tgccgggatg cctggcaatg
       61 gtgttactga acgcagccgt cagttacggc attgtcagac tgaacgcacc gatgactgcc
      121 gccttcaaca tgaaacagac tgtggatgca ttttttgaca gcgccagtca gaaacaactg
      181 tcagaagcac agtcaaaagc cctttcagcg cgttttaaca cggcactgga agccagcctg
      241 caggcgtggc agcagaaaca ccacgcggtc attctggtgt cgcctgccgt ggtacagggg
      301 gctccggata tcacccggga aatccagcag gatattgccc ggcgaatgag agcggaacca
      361 tgagatgccg ggggctgatt gccctgctga tatgggggca gagtgtggcc gccgccgatc
      421 ttggcacctg gggtgatctg tggccggtaa aggagccgga catgctgacg gtgatcatgc
      481 agcgcctgac ggcactggag cagtccggtg agatgggccg gaaaatggat gcctttaagg
      541 agcgggtgat ccgcaacagt ctgcggcccc ctgccgtgcc cggtatcgga cgcacggaga
      601 aatacagcag ccggttgttt gacccgtccg tcagactggc tgcggatatc cgggataacg
      661 aagggcgggt ggccggcttt gagtcagcct acgtggccct gtcgcgtatg aagcagcatg
      721 tgcaggtgta caccgataac cgtcagggct ggacggatgc catcaacaat gccgtacaga
      781 aaggaactgc ccacgatgtg tttgagccga aaccggaccg ggaggtcatg aatgcagagc
      841 ggctgttcag tacggcgcgg gagctgcggg acgtggcggc aggccgtgcc gttctccgtc
      901 aggcggggct ggccggggga gacagtcctg cacggtttat tgctccggga cgtaaatatc
      961 cgcaaccgta tgtggcactg ccggcgtttg accgtaacgg caagtccgcc ggtatctggc
     1021 tgaacccgct gaccacggat gacggaaacg ggctgcgggg attcagcggt gaaggtcggg
     1081 taaaaggcag cggggatgcg cagttcgtgg ccctgcaggg cagccgtaac ggagaaagcc
     1141 tgctggctga taatatgcag gatggcgtcc ggattgcccg tgataatcct gacagtggtg
     1201 tggtggtaag cattgccggt gaaggccgtc cgtggaatcc cggtgccatc accgatggcc
     1261 gcgtgtgggg ggatatcccg gacaacagcg tccagccggg agccggaaat ggcgaaccgg
     1321 tcacggcaga ggtactggca cagcggcagg ctgaagaggc catccgccgt gaaacggaac
     1381 gtcgcgcaga tgaaattgtc cgtaaaatgg cagagaacaa acctgacctg ccggacggca
     1441 aaacagagct ggctgtcagg gaaattgccg ggcaggagcg tgaccgggct gccataactg
     1501 aacgggaagc cgcgctgccg gagagtgtgc tgcgtgaatc acaacgggag cgggaagcgg
     1561 tccgggaggt tgcccgggaa aatctgctgc aggagcgtct gcagcagatg gagcgggata
     1621 tggttcgtga cctgcagaaa gagaaaaccc cgggcggaga ctgatacagg aagacaaaca
     1681 ctgatgacaa cggataacac gaacacgaca cgtaacgatt cactggctgc acggaccgat
     1741 acctggttgc agtcatttct ggtctggtca cccggacagc gggatatcat caaaacggtg
     1801 gcactggtgc tgatggtact ggaccacatc aatctgatat tccagctgaa gcaggaatgg
     1861 atgtttctgg ccgggcgagg agcctttccg ctttttgccc tggtgtgggg gctgaatctg
     1921 tcccgtcatg cgcatatccg gcaaccggcc attaaccggc tgtggggatg gggaattatt
     1981 gcccagttcg catattatct ggcaggcttt ccctggtatg aggggaatat cctgtttgcc
     2041 tttgcagtgg cggcacaggt gctgacgtgg tgtgaaacgc gcagtgggtg gcgtacagca
     2101 gcggccattc ttctgatggc cctgttgggg gcctttgtcc ggcaccagtt acggcattgc
     2161 cgggctgctg atgctggcag tcagccaccg actgtaccgg gcggaagaca gagcggaacg
     2221 tctggcgctg gtcgcctgcc tgctggctgt tattccggcg cttaaccttg ccaccagtga
     2281 tgcagcggcg gtggccggtc ttgtgatgac ggtgctgacc gttggtctgg tgtcgtgtgc
     2341 aggaaaatca ttaccccgtt tctggcccgg ggattttttc ccggtattct acgcctgtca
     2401 tctggctgtg ctgggcgttc tggcgctgtg acgggtgtgg tatctttgac cgtaacagga
     2461 tgattcgtca gaggcagaac acagcatgac agagcagaag cgaccggtac tgacactgaa
     2521 gcggaaaacg gaaggggaga cgcctgtccg cagccggaaa accatcatca atgtcaccac
     2581 gccaccaaag tggaaggtga aaaagcagaa gctggccgag aaagcagccc gggaagcaga
     2641 gctggcggca aaaaaagcgc aggccagaca ggcactgtcc atttatctga acctgcccac
     2701 gctggatgag gccgtgaata ccctgaaacc ctggtggccg ggattatttg acggtgacac
     2761 cccccggctt ctggcctgcg gtatccggga cgtgttactg gaagacgtgg cgcagcggaa
     2821 tatcccgctc tcgcataaaa aactgcgcag ggcgctgaag gccatcaccc gttcagaaag
     2881 ctatctgtgt gccatgaaag ccggtgcctg ccggtatgac acggaagggt atgtgacgga
     2941 gcatatttct caggaggagg aagcgtatgc ggcagcgcgt ctggataaaa tccgccgcca
     3001 gaaccggata aaggcagaac ttcaggccgt gcttgatgag aaataaaaaa aagcctccat
     3061 cagagaggag gcagggaaat aaggcttaaa aggaaataac ttccgcttcc gaaaggaagc
     3121 cagtccactg aattatttat cacaataata ctgacaacta aagggggaca agaaaatgaa
     3181 tggattcaga aacagttcac ggaacggcca ggtctggcgt taccagcgtg ccggcgggcg
     3241 ggccgttatt ctggaagtca gtggacgctg gatggaagcg gcagaagcat ggcgacgggc
     3301 tgcctgtgtt gcgcccagga cagactggca gcagtttgcc cgaaaaaggg ctgaacactg
     3361 ccaccggcgc tgccggggaa gggtgtaacg aaaaaagcag ccttcattac tccaggagtc
     3421 agaaggctga aaggagttta tgagatttgc tttttttaat atccggtcgg gcagtatgac
     3481 cggacagttc tgagtagcac agagtttttt ccgtatta
//
LOCUS       DAZPLI010000269         3490 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZPLI010000269 DAZPLI010000000
VERSION     DAZPLI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.272"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            69..1010
                     /locus_tag="ACHUU3_004238"
                     /pseudo
     CDS             69..1010
                     /locus_tag="ACHUU3_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            1010..1108
                     /locus_tag="ACHUU3_004239"
                     /pseudo
     CDS             1010..1108
                     /locus_tag="ACHUU3_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            1108..2429
                     /gene="hipA"
                     /locus_tag="ACHUU3_004240"
                     /pseudo
     CDS             1108..2429
                     /gene="hipA"
                     /locus_tag="ACHUU3_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            2613..2714
                     /locus_tag="ACHUU3_004241"
                     /pseudo
     CDS             2613..2714
                     /locus_tag="ACHUU3_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            3075..3490
                     /locus_tag="ACHUU3_004242"
                     /pseudo
     CDS             3075..3490
                     /locus_tag="ACHUU3_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
BASE COUNT          980 a          711 c          860 g          939 t
ORIGIN      
        1 taggtgggca ttgcacatat ttccgacgaa tagatcacaa tttatgctat tttgattttc
       61 acggttgcac atcaacagtt aatttgcgta aatcctatgg actgacaacg aaattcagta
      121 gattgacgct gggtgttttg ctggcactaa gcggttcagc gtctggtgcg agtctggaag
      181 ttgataatgg tcagattacc aatattgata ctgatgttgc ttatgatgcc tacctggttg
      241 gctggtatgg cactggagtt cttaatattt tggctgacgg taatgcctac ttaaccacta
      301 ttactaccag cgtcattggc gctaatgagg attcggaggg taccgttaat gttttgggtg
      361 gcacctggcg attgtatgat agcggaaata atgcaaggcc tttaaatgtg ggtcaatccg
      421 gaacggggac gctgaatatt aaacagaaag gtcacgttga tggaggctat ttaagactag
      481 gttcctcgac aggaggcgtc gggacggtca atgttgaggg agaagactct gttctgacga
      541 ccgaattatt cgaaataggg agttacggta cgggctcatt aaatattacg gataagggtt
      601 acgtcacgag ttcaatagtc gccattttag gatatcaagc gaacagtaat ggtaaggtta
      661 tcgttgaaaa gggtggcgag tggctaataa aaaataatga ttcctcaatt gaatttcaaa
      721 ttggtaatca aggagctggg gaggcgacta ttcgcgaggg tgggctgatt acggctgaaa
      781 atacgattat cggtggcaat gccaccggta tcggaaccct gaatgtgcag gatcaagact
      841 ctgtcatcac ggtacgcaga ctctataatg ggtatttcgg taatggcaca gtcaatattt
      901 ccaataatgg tctgattaat aacaaagaat attcattggt gggcgttcag gacggttccc
      961 acggtgtcgt caacgtgacc gataaagggc attggagttt cctcggaact tttttaagat
     1021 tttacagtcg cttgaactct caatgacgct atgcgacgcg aaaaatgcct cgccagaagc
     1081 aacagaacag caagatctgg agtggtaatg cctaaacttg tcacttggat gaacaaccag
     1141 cgggtaggcg agttaacgaa gttagccaac ggcgcgcaca cctttaagta tgcaccggag
     1201 tggttagcca gccgttatgc cagaccgttg tcactttcgc tgccattgca gagggggaat
     1261 atcacctctg atgccgtatt taacttcttc gataacctgt tacccgatag cccgattgta
     1321 cgtgaccgga tcgttaaacg ttatcatgcc aaatccagac aaccgtttga tttgttgtca
     1381 gaaatagggc gagacagcgt tggtgccgtg acgttaatac ccgaagacga aaccgtaacg
     1441 cgtccgataa tggcatggga aaagcttact gaagccagac ttgaagaagt attaacggct
     1501 tataaagcag atatcccgct aggcatgatt agagaagaaa atgactttcg catctcggtt
     1561 gctggcgcac aggagaagac agcactgctc agaataggca atgactggtg cattccgaaa
     1621 ggaataacgc cgacgacgca catcattaaa ttaccgattg gcgaaatcag gcagcccaat
     1681 gcgacgctcg atctcagcca aagcgttgat aatgagtatt actgtctgct gctggcgaaa
     1741 gaacttgggt tgaatgttcc ggacgcagaa atcattaaag cgggaagggt gcgcgcgtta
     1801 gcggtcgaac gttttgacag gcgttggaat actgagcgaa cggttttact tcgcttgcca
     1861 caggaggata tgtgtcagac attcggttta ccttcatcgg tgaaatatga atcagatgga
     1921 ggcccaggca tcgcgcggat catggcgttt ttgatggggt ccagcgaggc gctgaaagat
     1981 cgctatgatt ttatgaagtt tcaagtgttc cagtggttga ttggcgcaac ggatggccat
     2041 gcaaaaaact tctccgtatt tattcaggct ggcggcagtt atcgactcac gccattttac
     2101 gacatcattt cagcatttcc ggtccttggc ggtacgggaa taacatcagc gatctcaaac
     2161 tggcaatggg gcttaacgca tccaaaggca aaaaaacggc aatcgataaa atttatccgc
     2221 gacatttttt ggcgacagca aaggtgctga gattcccgga agtgcagatg catgaaatcc
     2281 taagtgactt tgccagaatg attccggcag cactggataa cgtgaagact tcattaccga
     2341 cagattttcc ggagaacgtg gtgacggcag ttgaaaccaa tgtgttgagg ttgcatggac
     2401 ggttaagccg agaatacggt agtaagtgag atatggggtt tggtcgttgt taatgagcaa
     2461 tcatgaccgg ccaacgactg gattctccct tggtttatgt ttgtacgcat aatgtatatt
     2521 atgttaaatc atgtctgagt gatgcgaagt gcttttctgg tagtcgttat tcgttcagat
     2581 taggtgtcct tgtgatttaa agatgttatc gtggcacgaa tatactgcca ttccaggctc
     2641 gttatattgc tctcgggcag tccgtagctg gtactgcaaa cgctgatgcg accttcaaag
     2701 ttcaatatct ataattcaat cttaaggtgg ctatacgtca tctgaaattg aaacagggtg
     2761 tgtgtggcat ggctacgcac acccgagcaa ttcaccaatg tgaatctcat tattaatgag
     2821 gagtggtttg tatttggagc gctatgaaaa atcacataat atctaaacta tcaataaaca
     2881 acagtagtta aacttactgt tatttaaaat atttactttg tagcaaggtt ttcctattaa
     2941 aagagaacca acatttatta caaacgcatt aagtctggaa acccggcgac tcgccaatgt
     3001 attagcaaca ttaatcgatt gtctgatgta acggttttcc cgcttatcct cttaacacat
     3061 ggagaatgca tcagatgcaa actacgcgta ccccctactc tatttccttt atggcgacag
     3121 tgttactttt attgctcttt gcttgccata gcaccgttgc caacgcagct gtcgcactgg
     3181 aatacgctgc ggataatcaa cgctactaat catcagttac caggcgaccg tgaaagcctg
     3241 ttctgggtga atgttaaagc tattcctgct atggaaaaag accagaagaa tgagaataca
     3301 ttacagctgg caattattag ccgcataaaa atgttttatc gccccacgaa cctggcaatg
     3361 gcacctgaag aagcgcctgc aatgcttcgg tttcgtcgta gcggtagcaa actcactctt
     3421 attaatccaa cgccctattt cattaccgta acaaatatga aagcaggaaa tagcaatctc
     3481 ccaaacacca
//
LOCUS       DAZPLI010000270         3487 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZPLI010000270 DAZPLI010000000
VERSION     DAZPLI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.273"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            784..1470
                     /locus_tag="ACHUU3_004243"
                     /pseudo
     CDS             784..1470
                     /locus_tag="ACHUU3_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1803..2678
                     /locus_tag="ACHUU3_004244"
     CDS             1803..2678
                     /locus_tag="ACHUU3_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFR2084140.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            2686..3462
                     /locus_tag="ACHUU3_004245"
     CDS             2686..3462
                     /locus_tag="ACHUU3_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFR2084141.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
BASE COUNT          965 a          810 c          822 g          890 t
ORIGIN      
        1 aatcaattaa gaaaattgac gccagactaa ttattaataa tccagttatc tttttattca
       61 tatcatctcc tttccattgg ttaatttatc ggaaattcat ccccactaac taaaacgatc
      121 cacacaataa aactaaatct aattgcaggc aatcaattcc agtcgatata tcatctgtat
      181 ggaaatcatc gctcatcact catcgcagcc tgaaagccca gagcaacggg gcaggtctgc
      241 atataagtgg ttacacggca gaattccaga gggttctgtc actaaattca gtcactgaca
      301 aatttgcatg atgttgaata gacgagattt tgtgtcgaaa tagttgtttc agtaactgtc
      361 actcaatcgg tggtttatgt gactatattc gcaaaaactg gtcgcagaaa agtcctgttt
      421 tcagcttgga aacccggtaa tcagaataat ggatagggga acaaaaatct ctgtatagaa
      481 cgctccattg agggagagaa aacgtgtcat tttgacccgt tcggtattca gaacacacaa
      541 aaccgatcgc gggtcgcttt cgtgcatagt tatgcaacgc gctaaaaacg attctgacgc
      601 attttttatg tttaccctgt tttttcttgt ctttttgagt tttttgcgtc agagagcgtc
      661 tgagagggca cgtacaacgg gagttatggt aaatggatcg ggttttcggg aaggttcgac
      721 aggatttgcc gttgggtgta gtgtaagcga ctgaaaaaca aacgcccctg aaatcatgtc
      781 cagatccggc agaaccagcc tcatgcgcag ttctggctgg tttttgacgt tgatcgcgag
      841 ggggcagcga ttgactggag cgaccggaac gctcccgcgc cgaacatcac cgtaaaaaat
      901 cctgtgaacg gtcatgctca cctgctctac gcgctcaata tcgccgtgag aaccgcgccg
      961 gacagctcgg ttaaggcgct gaaatacgcc gctgcgattg aacgttcgct gtgtgagaaa
     1021 ctttgtgctg atgtgaacta cagcggactg atttgtaaaa atccgttcca cctggaatgg
     1081 caggtgatgg agtggcgaga ggaagcctat accctcgatg aactggctga ttatctcgat
     1141 ttgagcgcct cagcgcgccg tagcatcgat aaacattacg ggatggggcg aaactgccac
     1201 ctgttcgaaa tgacgcgcaa atgggcttac agggcgattc gtcagggctg gcccgaattc
     1261 tcacagtggc ttgatgccgt gattcagcgt gtcgaaatgt acaacgcatc gcttcccgtt
     1321 ccgctttcac cgcctgaatg tcgggctatt ggcaagagca ttgcgaaata cacgcacagg
     1381 aacttcacgc cggaaacttt cgcacagtat gtggctgata cgcacacatt aacgtacgtt
     1441 ttcgttccat tggccctcac cccccgataa cgacccaggt agcgcgccgt attccccccg
     1501 ccggccgttt tcttcgacat gtacatcagg cgcggaactc atcggggcgc gggaatgaaa
     1561 tcccattcag actccggata gattcaagca agcccactta tcgtcaaaat cggtgttgca
     1621 aaaacgggag tgaccataga tccgttttcc aagcggaccc cataatatcg gctggtaaac
     1681 atcactcgct agtgcattct ttttgttggt aatgataatc atttacattc attctttgat
     1741 gttttatcat aattaatgtt ttgtcaataa atatatggaa acatattgtt tcagatggga
     1801 atatgagaaa gttcatcctt atttctatga taacgttagc tagtgttagt ctgggagcct
     1861 gcagacagaa tgtcaccata aagcaggatg cgccagggca gaaagctttc ctgactgacg
     1921 ggcagattta cgatttgcac agtggcaaaa ttatttctag cagcgagctt ttggcggatc
     1981 ttgctaccgc tcgacatctt attattggtg aaaaacacga taatgccgag catcatcaga
     2041 ttgagctttg gcttattcaa aatttgctca tccagcgacc acaggggagt gtgttgcttg
     2101 agatgttgac gtcagagcaa cagccccgag ttaaccaagt taaatgttgg ctgaaagata
     2161 atcctgtggt tcgagatagc cgtgttcagg aactacttaa ctggcaaaaa gggtggtcct
     2221 gggagatgta tggcgacatc gtcatgcagc tattacgtgg gccttacccg ctgctcaatg
     2281 ccaacattgg ccgggagcag atgttggcac tgtacaaaaa aaacgaattt cctaaaggaa
     2341 aaaaatccac tgcacctgtg gtacaagaag ctctgcgtga aaccatcata tctatgcatg
     2401 aaggcaacct tgaaagccag caactgacgt caatgctctc tattcagcag cagcgggatc
     2461 gctacatggc ccgccagctt ctgagtgccc cggtaccgtc gttgttgatt gcaggtggtt
     2521 atcacgcgtc aaaaagcatg ggcgtaccgt tacacatgga agatcttgcc accggcaccc
     2581 atccagtggt gttgatgctt gcagaaaaag gaatgaacat cacagtagat catgctgact
     2641 acgtgtggtt tgtagcgcca gatacaacaa aaaggtaaac catggatgat gaatattctc
     2701 cacttctcac agtcggtaaa atggagcagc tggtttatct gttctctact gcttcatgga
     2761 ctgattttcc ttgcgtttat ctggcgtttt tccgaagttc agcccgctat gtctcctgca
     2821 ccggcgatca tgctgcaatg ggcagaaatt atagaggctc cttcttctcc actttcgctt
     2881 ccggtgggaa tcgctcaaca ggagtccgca gtgacagaag aaaagcaaca aactgaggac
     2941 agacaacaac gtcctgtcac tgaagacagt gatgcaacca ttgaaataac taggaagaaa
     3001 aagtcatccg atggagaaaa gaaaaagaca cgtccacctc gtaagataaa ggctcaaacc
     3061 agcgacagca accccacagc cgtttccagt aatgctgcac ctcaggctct ggtcgaatcg
     3121 tcccgtattg ctgcgccttt taacagtgac tcaacaaagc gagataattc tgaggcatct
     3181 tgggaaagca gagtaaaagg ccacctaaac cgttataaac gttaccctgg cgatgccaga
     3241 aaacgtgcac gaaccggcac agccgtcgtg acttttaccg taaacacgga aggcactatt
     3301 gtcagtagtt tcttggaaat ctcttcagga accctttctc ttgaccgcga agctattgct
     3361 gttttagaac gcgctcaacc acttcccaaa ccaccgcctg aaatattgga gggaggatta
     3421 tttaaagtaa aaatgccgat tacctttaag ttgaaggagt aaagggataa ttggccgttc
     3481 aagttca
//
LOCUS       DAZPLI010000271         3429 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZPLI010000271 DAZPLI010000000
VERSION     DAZPLI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.274"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..140)
                     /locus_tag="ACHUU3_004246"
     CDS             complement(<1..140)
                     /locus_tag="ACHUU3_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR2084142.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IF"
     gene            458..1735
                     /locus_tag="ACHUU3_004247"
     CDS             458..1735
                     /locus_tag="ACHUU3_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFR2084143.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            1762..3248
                     /locus_tag="ACHUU3_004248"
                     /pseudo
     CDS             1762..3248
                     /locus_tag="ACHUU3_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT          876 a          770 c          865 g          918 t
ORIGIN      
        1 ataaagatat ttgcgccagc tttggccaac gccatggcaa atgcctggcc taaaccgcta
       61 ttcccaccgg taacaattgc ggttttacct ttcagtgaga aaaaatccat tgagaacgca
      121 ttgagagatt cgattgacat agttagctcc aatttccgta aggcaaaaaa aagaaaggta
      181 acttcccccg cgagagggaa gttacctttc tcattatctc tggtaactgg agataatttt
      241 tagcataacg gcataactgc taatgtgatc gtaatcacag tgtgatattc gtattgtcaa
      301 atagttttaa aaagcagaag cgtgattact ctcacacaac acctctgctt tccatgttat
      361 ttcttattgc aattactaga gacctgagaa aagtaatcca cctgaaaggg cggcttactt
      421 ttctcttttt tttgtctatt acaaatggga atacgatatg caacacaact catatcgccg
      481 ttggataacc ctcgcgataa ttagttttag cggcggcgtt agttttgacc tggcttattt
      541 acgttatatt tatcaaattc ccatggcgaa atttatggga ttcagcaata ccgagatagg
      601 tttaataatg agtacctttg gtattgcggc tattattctt tatgccccca gcggcgttat
      661 tgccgataaa ttttcacacc gcaaaatgat tacttccgcg atgatcatta ccggattact
      721 gggtctgtta atggcaacgt atccaccgct gtgggtaatg ctctgtattc aggtcgcctt
      781 tgcgataacg acgattttaa tgctgtggtc ggtgtcgatt aaagccgcct cgttgctggg
      841 cgatcatagt gagcaaggga aaattatggg ctggatggaa gggctgcgcg gcgtcggtgt
      901 aatgtcgctg gcggtgttta ccatgtgggt cttttctcgc tttgcaccgg atgacagcgc
      961 cagcctgaaa acagtcatta tcatctacag tgtggtttac atcttgttgg ggattctgtg
     1021 ctggtttttt gttagcgata acaacaacct gcgcagtgcc aataacgaag aaaaacagtc
     1081 attccagctt agcgacatcc ttgccgtttt gcgtatcagc accacctggt attgcagcat
     1141 ggtgattttt ggtgtcttca caatctacgc cattctgagt tactccacca actatctgac
     1201 cgaaatgtac ggcatgtcgc tggtggcagc gagctacatg gggattgtga tcaacaaaat
     1261 cttccgcgcg ctgtgcggcc cacttggagg cattatcacc acctacagta aagtgaaatc
     1321 ccctacccgc gtgattcaaa tcctttccat catcggtttg ctggcgttga cagccctgct
     1381 cgtcacgaac tctaaccccc aatcggtcgc gatggggatt ggcctgattt tactgctggg
     1441 attcacctgt tacgcctcac gcgggctgta ctgggcctgc cccggcgaag cgagaacacc
     1501 gtcttacatt atgggcacca cggtaggtat ttgttcggtg attggattcc tgccggatgt
     1561 cttcgtttac ccgattatcg gccactggca agacaccctg cccgctgcag aagcctaccg
     1621 caatatgtgg ctgatgggca tggcggcgct cggcatggtg attgtcttta cctttttgct
     1681 gttccaaaaa attcgtactg ctgatagcgc ccccgcaatg gctagcagca agtaagccag
     1741 gaaaattaca ggagaaaaat catgtcgaaa aaatacatca tagggattga tggcggaagt
     1801 cagagcacaa aagtggtgat gtacgatctg gaaggtaatg tggtttgcga aggtaaaggc
     1861 ttattacagc cgatgcacac gcctgatgcc gatactgcag aacatcctga cgacgattta
     1921 tgggcatcat tatgttttgc aggtcacgat ttgatgagcc agtttgccgg gaataaagaa
     1981 gatattgttg gcatcggcct gggctccatc cgttgctgcc gtgcgttatt gaaagccgac
     2041 ggcacgccag ctgcgccgtt gattagctgg caggatgcac gcgttacacg cccttacgaa
     2101 catacgaatc ctgacgtggc gtatgtaacc tctttttcgg gttatctgac gcatcgctta
     2161 accggcgaat ttaaagataa tatcgccaac tattttggtc agtggccggt ggattataag
     2221 acctgggcat ggagcgaaga tgccgcagtc atggagaagt ttaatatccc ccgtcatatg
     2281 ctgtttgatg tgcaaatgcc aggcactgtt cttgggcata tcacacctca agccgcgctg
     2341 gcgacgcatt tcccggcagg actgccggtt gtttgtacca ccagtgataa accggtagaa
     2401 gctctgggag ccggattact ggatgatgaa actgcggtaa tttctcttgg cacttacatc
     2461 gcattgatga tgaacggcaa agccttgccg aaagatccgg tggcgtactg gccgattatg
     2521 tcttctattc cgcaaacatt gctgtatgaa ggttacggta ttcgcaaagg catgtggacg
     2581 gtgagctggc tgcgcgacat gttaggcgag tcgttaattc aggatgccag ggcgcaggat
     2641 ctttcaccgg aagatttact caacaagaaa gcctctagtg tgccacctgg ctgtaatggg
     2701 ctgatgacgg tgctggactg gctgaccaat ccgtgggaac cgtacaaacg cgggattatg
     2761 atcggctttg attccagcat ggattacgca tggatatatc gttcgatatt ggaaagcgtg
     2821 gcgctgacgc tgaagaacaa ttacgacaat atgtgtaatg aaatgaatca ctttgcgaaa
     2881 catgtaatca ttactggcgg cggttcgaac agcgatctgt ttatgcagat ttttgccgac
     2941 gtgttcaacc ttccggcacg ccgtaacgcc attaacggtt gtgcaagcct gggggcagcg
     3001 attaatacag gggtaggtct ggggctatac ccggattacg caacggctgt cgataaaatg
     3061 gttcgcgtga aagatatctt tataccgatt gagagcaatg ccaaacgcta cgacgcgatg
     3121 aataaaggca ttttcaaaga cctaaccaaa catactgatg tgatcctgaa aaaatcgtat
     3181 gaagtgatgc atggggaatt ggggaattgg ggaatgcgga ttcgatccag agctggtcga
     3241 atgcgtgaat aaggtaggcc gggcggaaaa ctgcccgacc tgaacatacc tgaatggtta
     3301 tccccgctgg cgcggggaac ataagttcga caacttaaaa aatgataaca agtccggttt
     3361 atccccgctg atgcagggaa cataataaaa actttaacta gttcggttta tccctgtttt
     3421 tacaaggaa
//
LOCUS       DAZPLI010000272         3328 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZPLI010000272 DAZPLI010000000
VERSION     DAZPLI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.275"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(134..307)
                     /locus_tag="ACHUU3_004249"
                     /pseudo
     CDS             complement(134..307)
                     /locus_tag="ACHUU3_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581267.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            462..581
                     /locus_tag="ACHUU3_004250"
                     /pseudo
     CDS             462..581
                     /locus_tag="ACHUU3_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332787.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            805..1035
                     /locus_tag="ACHUU3_004251"
     CDS             805..1035
                     /locus_tag="ACHUU3_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084144.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            1087..2448
                     /locus_tag="ACHUU3_004252"
     CDS             1087..2448
                     /locus_tag="ACHUU3_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFR2084145.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAETEDLGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            2495..3058
                     /locus_tag="ACHUU3_004253"
     CDS             2495..3058
                     /locus_tag="ACHUU3_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR2084146.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDRILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            3058..>3328
                     /locus_tag="ACHUU3_004254"
     CDS             3058..>3328
                     /locus_tag="ACHUU3_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084147.1"
                     /translation="MTQGWREDKCPSVRAGKRPRAGGAERGCAHAPPLSLRGRSDGGF
                     CLFRPVICLLLMMLLFLLLLLLLLLLLLLLLLLLLLLLLLLLFFFL"
BASE COUNT          736 a          947 c          977 g          668 t
ORIGIN      
        1 cgcaccttcg gctcccggct gcctggcggc atccggtcgt caggcccgac tccgacaggc
       61 agctgctggc gcacccgcca gtctccgccg aacccgctgg cgcggtttcg ggctgtcgct
      121 acggtccgat gcgtgaaaac ccccggcttg cagcgaccgt tccggtcgcg ccgcctctgt
      181 cgcgccgtgg cctgcgctcc cggcatcccg gcaaaaagac accgggatgt aaggcgcaac
      241 gccgtttcgc gcacgcgctc ttcgtcgttg cagcgggccc ctgctcattg cctgccgggt
      301 gccacacacc cgtatgagtc cctgtcgccg ggggctgata cagccacaac ctgccgcact
      361 gactgtccag ggtaaatgaa cagcccacaa aattttttac ccaaaacccg gtaaaaaatt
      421 ttgcgttctg cccaggacag gtgcgtcagg ccgtggcagt gatgcccctt gcggcgaagg
      481 ttctcaaacg ggtgaagttc ataccacagg gaatatagca gcaggttccc gcgaaacgtc
      541 aaaaccgcgg ctcccgaaac tcaacgggtg atgttcttta aaaatcagga tttatttcag
      601 gctccggcaa tacgcattta tgtgttctgt cagtcatccg gctggcaggt aatgagaacg
      661 caggaacaga aggaggaaaa caccggagaa cgtcagacgc agatatgaaa aaagcaggcc
      721 ggggaggacc tgcttaatcc acaccgcaaa aactgttcga gcagtttctg cggttccatt
      781 caacagtcat aaggagtttc atttatgacg gtaagtattg tttcaccttc cgcagcggct
      841 gtaaagcccc gccgtcatcc ccgtttcaga cgcgaagaca tccccgcgcc ggaaattgac
      901 cccgtgctga aagccttcgg gcgacacata gcccggagtt ttcaccgggg gcgtggtgta
      961 cacatccccg ccatgaaaaa cacggccttc gggcaggtgc tccgcacact cgaactgaaa
     1021 cgcgctttta actgatgagg tgagccgccc cgcgaggggc ggcaacagac ggaaggagaa
     1081 cattttatga cattagcagc aatcaccatg accgccccgg aggccgccag ccctgtgcag
     1141 atgtaccgcg cgacctactc accggatgac aacaaactgc gcctgtatgc cgcgtcacgt
     1201 cttgacccgg agacgtataa aaaagtgcat gatgccggtt ttcgctgggc acccaaacag
     1261 gcgctgtttg tcgcgccagc ctggacaccg ggccgggaag acgtgctcct ctcacttgcc
     1321 ggagagattg aggatgaaga cagcacgctc gctgaacgtc aggaagcacg ggcggagcgg
     1381 tttaccggat acagcggaaa gcgggccagt gaatccgcac aggcacttga tgaagtggaa
     1441 agactggccg cgatgatccc gcccggtcag cccattcttg tggggcatca cagcgaacgc
     1501 cgcgcccgtc gtgatgcgca gcgtattgaa aacggcatga aacgtgccgt gatgctcttt
     1561 gaacgtgcgg aatactggga ggagcgggcg cggtcggcac tgcttcacgc gaagtataaa
     1621 gaacgcccgg acgttcgctg gcgtcgtatc aaaaaaatcg aagctgattt gcgcaaggct
     1681 gaaaagacca tcgcgcagtc gcagaaatat ctgacgatgt ggcgggctga atcgctggat
     1741 ctgaatatgg caaaactcat cagcagtcat gaccatatca gcgcctgttt cccgctggat
     1801 acgtatccgc gcccggcaga aaaaagccag tatgaaggaa gtcgatcgtt atggtcggcc
     1861 ctggatgatg acatcatcac cacggagcag gcccgcgaaa ttgcgatccg ctgtcatgaa
     1921 cggcagattc agcatcagca acgctgggtt aaccactatc agaaccgcct gatctatgag
     1981 cgtgccatgc tggacgaaag cggcggcgtg gttacccgga cacaggattt tgagccgggc
     2041 ggacaggttt tcagccgggg cgagtggctg actatcatcc gtgtgaacaa aagcaacggg
     2101 gcggtgagtt cagtcacaac gccgaattac agtttccttg ggtacagcgg cacgatgaaa
     2161 gtgacgcccg atcgcatcac ggactacaaa gcaccatcgg cagaagaggc tgccgtcgcc
     2221 agtcaggccg cgaagcgtcc gccggtagtc aactatccgg gggaaggttt ccgggaaatg
     2281 acaaaggcac agtgggccgc cctgccccgg gactgtaagg ccgtgcgcag tgtggcagag
     2341 acagaagacc tcggggcata ccgctaccgc cgcacaatgg acaataattt ccgcctggtg
     2401 aatgtgtata ttaccgacat gaaaatcacg gaaatcccac agaaataagg tatatccccc
     2461 cgggaaatcc ccgggggatg acggagataa tgacatgcac agtcagttaa gagaacgtat
     2521 ccggctgatg cgcgcaaggc tggataacgc cgcgccggtt gctgaaatcc gggctgaatc
     2581 tcagcttttt gtgactcctg ccccggtctg tgatcgcctg gtgacgctgg cggaaatcag
     2641 caaccgtgat cgcattctgg aaccctctgc cggcaccggg gccatactgc gggcgattcg
     2701 ggataccgcg ccggaggcca tgtgtgatgc ggtggagatt aacagcgggc tggtccggta
     2761 tctgcgggaa aattttaacg gtgtcagggt tcagtgtggc gacttcatgg aatggcagcc
     2821 ggtgcagtat tacagccgga tcattatgaa tccgccgttc agtcacgggc aggatatccg
     2881 gcatatcctg cgggcttttt ccctgttgcg tccggggggc gttctggttg ccgtctgtct
     2941 caacgggcca cgccagcagg agaagctgtt accgttttct gacgtccgcg aggagctgcc
     3001 gcgcggcacg tttgcttata ccgatgtccc gacgatgatt attcgtttgc gtgcctgatg
     3061 acacagggct ggcgggagga taaatgcccg tctgtccgcg ccgggaaaag accgcgcgcc
     3121 ggtggcgctg aaaggggctg cgctcacgcg ccgccccttt cattacgcgg acgttctgat
     3181 gggggcttct gtcttttcag acctgtcatt tgtctgttac tgatgatgtt gttgttcctg
     3241 ctactgctac tgctactgct actgctactg ctactgctac tgctactgct actgctactg
     3301 ctactgctac tgctcttctt tttcctgc
//
LOCUS       DAZPLI010000273         3327 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZPLI010000273 DAZPLI010000000
VERSION     DAZPLI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.276"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(40..240)
                     /gene="glgS"
                     /locus_tag="ACHUU3_004255"
     CDS             complement(40..240)
                     /gene="glgS"
                     /locus_tag="ACHUU3_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR2084148.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            509..1138
                     /locus_tag="ACHUU3_004256"
     CDS             509..1138
                     /locus_tag="ACHUU3_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFR2084149.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1165..2826
                     /locus_tag="ACHUU3_004257"
     CDS             1165..2826
                     /locus_tag="ACHUU3_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFR2084150.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3065..3124
                     /locus_tag="ACHUU3_004258"
     CDS             3065..3124
                     /locus_tag="ACHUU3_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR2084151.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
BASE COUNT          943 a          770 c          777 g          837 t
ORIGIN      
        1 ctaatacaaa cactaaaacg ggccatcagg cccgttatat cagtgctcta actccagctc
       61 tcttgcctgc atcatctgtt ggcaatacca ggcataacgt gcgcaaaacc aggttctatg
      121 ttcttcatcc atgttaccag taacggcaag aatatcgacc gggcgacctt ctgcgtgcat
      181 tctggcaaaa ctacgcgcca ggaaatcgaa attatttaaa gaattaagac tatgatccat
      241 aagcacatcc tccggttatc gcataccttt taaggccata tgctttttct gatttcagta
      301 ttccccaatc agtgtagtgc ctttaaacat aacgtgcgta aatatattgt ccccgatcac
      361 actttttagt gaaaaataaa aaactatatt aaaaaacttt ctcttccaga acttctctaa
      421 catgtgttat tattaacaaa gataaatgta agcaaggaaa ctatcccaaa gtaaatattg
      481 cgtcactaaa tggacattgg agtgacatat gattttattc gccgactata atacccctta
      541 tcttttcgct atttcctttg tactgttgat cggcctttta gaaattttag cgcttatctg
      601 cggtcacatg ctctccggtg ctcttgatgc acatcttgac cattataatt ccataaccac
      661 aggtcatatt agccaggcac ttcattatct caatatcgga agattgccag ctctcgtcgt
      721 tctctgcttg ctggctggtt tttttggtct tattggcatc ctgttgcagc atgcctgcat
      781 catggtctgg caatcaccac tgtcgaacct gttcgtggtt cctgtcagtt tactgtttac
      841 gattattgcg gtgcattata ccggtaaaat agttgcgccc tggattccac gcgaccatag
      901 ttcagctatt acagaagaag aatatattgg tagcatggca ttaattaccg gtcatcaagc
      961 cacttcaggt aatccctgcg aaggaaaact caccgatcaa tttggccaaa tacattattt
     1021 actactggag cctgaagaag gaaaaatttt cactaaagga gtcaaagtgt taattatttg
     1081 ccgactttct gcaacgcgat atttggcgga aaacaatccc tggccacaaa tattgtaatt
     1141 tattaatcaa aaggaaattt aataatggat gatattgtta attctgtgcc ctcctggatg
     1201 tttaccgcga ttattgccgt atgcattctg tttattattg gaattatttt cgccaggctc
     1261 tatcgtcgcg cttcggcaga gcaagctttt gttcgtactg gtttaggtgg gcaaaaagtg
     1321 gtgatgagcg gtggcgcaat cgtgatgccg atctttcatg aaataatccc catcaatatg
     1381 aatactctga agctggaagt cagccgctca accattgata gcctgattac gaaagatcgt
     1441 atgcgtgtcg atgtagtagt cgctttcttt gtacgggtaa aaccgtctgt agaaggcatc
     1501 gccactgcgg cgcagacgtt ggggcaacgc acgctatcac cagaagactt acgtatgttg
     1561 gttgaagata aatttgtcga tgccctccgt gcaacagctg cacaaatgac catgcatgag
     1621 ttacaggata cccgcgagaa cttcgtgcag ggagtacaaa atactgtcgc agaagaccta
     1681 tcgaaaaacg gtcttgagct ggaaagcgtt tcacttacca actttaacca gacctcgaaa
     1741 gaacatttca atccgaacaa tgcctttgac gccgaaggtt taaccaaact gactcaggag
     1801 acggagcgcc gtcgtcgcga acgtaacgaa gttgaacagg atgtagaagt tgcggtgcgt
     1861 gagaaaaacc gtgatgcact ttcgcgcaag ttggagattg aacaacaaga agcgtttatg
     1921 acgcttgagc aggagcagca ggttaaaacc cgtactgccg aacagaatgc acgtattgcg
     1981 gcttttgaag ctgaacgtcg tcgtgaagca gagcagacgc gaattctggc tgaacgacaa
     2041 attcaggaaa cggagatcga gcgcgagcag gccgtccgct caagaaaggt tgaggctgaa
     2101 cgtgaagttc gtattaaaga gatcgaacag cagcaggtca ccgaaatcgc caaccagacg
     2161 aaatcgatcg ctattgccgc caaatcggaa caacagtccc aggcagaagc gcgtgctaac
     2221 cttgcactcg cagaagcagt aagtgcgcaa caaaacgtag aaaccactcg ccagaccgca
     2281 gaagccgatc gtgctaaaca agttgcccta atcgctgccg cgcaggatgc agaaaccaaa
     2341 gcggttgaac tgaccgtgcg ggcgaaagca gaaaaagaag ccgcagagat gcaggcggcg
     2401 gctatcgttg agttagccga agctacacgt aaaaagggtc tggcggaagc agaagcacaa
     2461 cgtgcgctga acgatgctat caacgtactt tctgatgaac aaaccagcct taaattcaaa
     2521 ctggccttgt tgcaggcgct acctgcggta atagagaaat ccgttgagcc gatgaagtcc
     2581 attgacggta tcaagattat tcaggtcgat ggtctgaatc gtggcagcgc tgcgggtgat
     2641 gcaaacacgg gtaatgtggg gggcggaaac ctagcggaac aagcattatc agccgctctc
     2701 tcttaccgca cacaggcacc gctgattgac tccttgctca atgaaattgg cgtttcaggc
     2761 ggctcactgg cggcattgac ttcaccctta acctcaacaa ctcccgtcgc cgaaaacgta
     2821 gaataaattt tcagctatga acgtccctgg cacaaaaagc cggggatttt ttatatctgc
     2881 gttccgctaa aaactgcaaa tgatcaggcc gttgcaacgt tttgcgtgac cgctcgggga
     2941 aggcaaaact gcctctggga aagcgttgcg cgaggtccgg cgctcatcaa caatcgggga
     3001 gtcagcaaag ggctgaaacg ggaaagcccc tcccgaggaa ggggccttac agaaggaaag
     3061 ggttatgatg aagctcgtca tcatactgat tgtgttgtta ctcgtaagtt tcgcagctta
     3121 ttaacagcca atcagagggg ggagaaatcc tccctcaccc ttgttccttt actttaggtt
     3181 gaaaaaaaat cagcgtcaag aaggtttcat taacacatat ccctggccca aaaagccggg
     3241 gattttttat atctgcgttc cgctaaaagg tgcaaatgct caggacgttg cagcgttttg
     3301 cgtgatcgct cggggaaggc aaaactg
//
LOCUS       DAZPLI010000274         3322 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZPLI010000274 DAZPLI010000000
VERSION     DAZPLI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.277"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..320)
                     /gene="fliZ"
                     /locus_tag="ACHUU3_004259"
     CDS             complement(<1..320)
                     /gene="fliZ"
                     /locus_tag="ACHUU3_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFR2084152.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTV"
     gene            complement(366..1085)
                     /gene="fliA"
                     /locus_tag="ACHUU3_004260"
     CDS             complement(366..1085)
                     /gene="fliA"
                     /locus_tag="ACHUU3_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFR2084153.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1250..2824)
                     /locus_tag="ACHUU3_004261"
     CDS             complement(1250..2824)
                     /locus_tag="ACHUU3_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFR2084154.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGKNLDVHQVQDTQGNPVPNSFAAKTSDGTYIAVNVDAATGNTSVITDPNGKAVEWAV
                     KNDGSAQAIMREDDKVYTANITNKTATKGAELSASDLKALATTNPLSKLDEALAKVDK
                     LRSSLGAVQNRFDSAITNLGNTVNDLSSARSRIEDADYATEVSNMSRAQILQQAGTSV
                     LAQANQTTQNVLSLLR"
     gene            3079..3322
                     /locus_tag="ACHUU3_004262"
                     /pseudo
     CDS             3079..3322
                     /locus_tag="ACHUU3_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
BASE COUNT          851 a          789 c          714 g          968 t
ORIGIN      
        1 accgtacctg ggctcatttc agtttgctca aaaagaaatt gcttaaagcc gataatatcg
       61 aaaaaatcac tctgcgtttt gcagtgtaaa tcaccacaaa atcggcacaa tgccgcccat
      121 gatttttgtt ccacttgcca gccattttca tcaagcagca tgtccaggcg ggaaatctcg
      181 attttattca ctattttgcc gtcgcgaacg agggtaatgc ggtcgagtaa tttacggcaa
      241 tgcgcgcaat gagtctggct gtgtttaaag tctttaagat agcggcttaa tggccgtctt
      301 ttcaggtgct gcaccatcat taagaactcc tggtagtcaa aattaaagtg cggcatttac
      361 tgacgttata acttacccag tttagtgcgt aaccgtttaa tggcctggct gtgtaactga
      421 ctgacccgcg attccccgac ctccagcacc gcgccaatct ctttgagatt cagctcttcc
      481 tggtaataga gggttaatac cagtttttcg cgctccggca acgtttcgat ggcttccatc
      541 acccgctggc gcagattact gtccagcagt tgttgtagcg ggttttctcg ctgatgatca
      601 tcagtaacca gttcgatgct atcgccgtgc tcttcgcgcc actcatcgta ggagaagagc
      661 tggctgttat tggtgtcgag caacatttgg cgataatcgg caatatcgat ccctaaacgt
      721 tccgctacct cagtttccgt ggcgttgcgg ccaagttcct gctccagttg ccctattgcc
      781 tgtgccactt cacgcgcgtt gcgtcgcacg ctgcgcggca cccagtcacg gctgcgaagt
      841 tcatccagca tagcgccacg gatacgctgc actgcgtaag ttgtaaatgc cgttccttgt
      901 agggcgtcat agcgttcgac ggcattaagt aacccaatgc cgcccgcctg tagcagatcg
      961 tcaagttcca cgctcgcggg cagtcgaacc tgcaggcgca atgcttcgtg acgcaccagc
     1021 gggacataac gctgccacag cgagtgttta tccattacac cttcagcggt atagagtgaa
     1081 ttcacgataa acagccctgc gttatatgag ttatcggcat gattatccgt ttctgcaggt
     1141 tttttaatcg gacgattagt gggtgaaatg aggggttatt taggggttac cggtaaattg
     1201 cggacagaaa aaaccccgcc ggtggcgggg tttgagcgat aagtgtaaat taacgcagca
     1261 gagacagtac gttctgcgtg gtctggttag cctgtgccag aacagaggta cccgcttgtt
     1321 gcaggatctg cgcacgagac atgttagaca cttcggttgc gtagtcagca tcttctatac
     1381 ggctacgggc agaagacagg tcgtttacgg tgttgccaag gttggtgatg gcagagtcga
     1441 aacggttttg tactgcaccc aaagaactgc gcaacttatc aacttttgcc aaagcttcgt
     1501 ctaatttgga taatggattt gtggttgcta aggctttcaa atctgaggca ctgagttcag
     1561 cacctttggt tgccgtctta ttcgtgatat tggctgtata aaccttatca tcttcacgca
     1621 taattgcctg tgcagaacca tcatttttta ctgcccattc aactgcctta ccattaggat
     1681 cagtaataac agacgtgtta cctgtagcgg catctacatt tactgcaatg taggtgccgt
     1741 ctgatgtttt agcagcaaat gaattaggta caggattccc ttgggtatct tgtacttggt
     1801 ggacatctag gttttttccc ccaaaataaa cagacattgg tgcatcatta taagctaatg
     1861 ctttacccat tttacccgca gctaaatcaa ctgatgtggc agaaccatta tcttcaacaa
     1921 atgcagttac acgaccacca aagtttgcta actgaacatc cttaccaccc acctgaactt
     1981 tttcataacc tgtagagttt tgtttcaata atgtaaaacc gttcgtgttg gtattattat
     2041 cttgaccaac aaaataagtc tgcccttcca cctgtacttg ataaactttt gttcctgcag
     2101 tgccggtcgc atcagttgta tcctttgtat cttgtacatt accggtacca aaataagttt
     2161 caacatcagc atttttataa ttgctaccga atgctttagc acctcccaca ctaccatcag
     2221 ttgcatttac cttagttaag tcaggagcgg cttcgctctt aagtgcaacc gcagaggaca
     2281 ttggaacttt tcctagtgct acgctaaagt tatcaagccc caaggtttta gcatcaattt
     2341 tatcaagggc aatctcaatg gtttcaccat cgttcgcacc tacttgaatt ttcatagtct
     2401 gatcagaagc cagaactttt acaccattaa attgtgtctg gttagaaaca cgatcaattt
     2461 cactaaggcg ttgtgtaatt tcatcctgaa ttgaattcag gtcggagtca gagtttgtac
     2521 cattagtggc ctgtactgac aattcacgaa tacgctgtaa gttattgttg atttcagaaa
     2581 gcgcaccttc agtggtctgc gcaacagaaa taccatcatt cgcgttacgg gaagcctgag
     2641 tcagaccttt aatatttgct gtaaaacggt tagcaatcgc ctgacctgct gcgtcatctt
     2701 tagcactgtt aatacgcagg ccagaagaga gacgttcaat ggcggagctc agagaagact
     2761 gagatttgtt caggttattc tgggtcaaca gcgacaggct gtttgtatta atgacttgtg
     2821 ccatgatctt ttccttatca attacaactt gatgttattg ggctgttgcc cacggtttct
     2881 caccgtaccc cttgtatcgg cacctgaatt tcgaacttta gaaaatttta gtttctctcc
     2941 tgttttttct taacggctga aatatccgta atatacaact ctactctgcg catcatcttc
     3001 gtgaatcaac tattaaactt tgcgcacaaa ttgccgataa cccgcttatc tactgttttc
     3061 aatcaaaagg aattaggtat ggcttcattt acatctcttg gcgttggttc gaatctgccg
     3121 ctggatacat tgctaaacaa cctgaccatc gctgaaaaaa aacgcttaaa cccaattact
     3181 cagcaacaga gtgacaatac cgcacgtctg acagcctatg gcacattaaa aatgtgcatt
     3241 agaaaaattc cagactgcaa atactgcgtt aaataaagcc gatctgttca aaagcactac
     3301 cgttaccagt agcaatgaag ac
//
LOCUS       DAZPLI010000275         3317 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZPLI010000275 DAZPLI010000000
VERSION     DAZPLI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.278"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(316..391)
                     /locus_tag="ACHUU3_004263"
     tRNA            complement(316..391)
                     /locus_tag="ACHUU3_004263"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(356..358),aa:Asn,seq:gtt)
     gene            complement(492..1979)
                     /gene="yeeO"
                     /locus_tag="ACHUU3_004264"
     CDS             complement(492..1979)
                     /gene="yeeO"
                     /locus_tag="ACHUU3_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFR2084155.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            2140..2215
                     /locus_tag="ACHUU3_004265"
     tRNA            2140..2215
                     /locus_tag="ACHUU3_004265"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2173..2175,aa:Asn,seq:gtt)
     gene            complement(2253..3203)
                     /gene="cbl"
                     /locus_tag="ACHUU3_004266"
     CDS             complement(2253..3203)
                     /gene="cbl"
                     /locus_tag="ACHUU3_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR2084156.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT          971 a          863 c          699 g          784 t
ORIGIN      
        1 tcttcttttt ctgattacag aaggcaaagt ggcaattacg catagtttcc cgataaagac
       61 gcgatagcga catcccgcat aaggcatttt tctctttatc tttgtacggt acttcatgga
      121 acagagtttt tgaccttgcg aatcgtgatg tctgttgggg aggggcaatt tgctcactga
      181 agcgtgagac tcgattaagc gcacgaaaca cagaaatcaa aaaacccggt cactttttta
      241 caaggtaacc gggtaaaaat aatttttatt ttttaactgt tttgagactc atagagatgt
      301 ctcaaaacta aaatttggct cctctgactg gactcgaacc agtgacatac ggattaacag
      361 tccgccgttc taccgactga actacagagg aatcgtgaga acgaggcgaa tattagcgat
      421 gcccacccac aatgtcaaag cctgtttttt aaatttgaaa tcgtttgctg aaataatctg
      481 cattttgtcg tttattccga cacaactggc tttttttcac acttttgcgg ttcggatcga
      541 gggtatttcc acagccaacg tccagtaacc attcgccagt aaaacagcac ggcccgcaca
      601 gcccagtcgg caaacattcc catccagaca ccaaccacac cccagccaag catgatgccc
      661 agcacataac cgacgacaac ccggcaaccc cacatgctca acatagaaac ccacatggcg
      721 taacgggcat cgcgagcacc tttaaatcca gcgggtagca cccatgaggc ggaccaaata
      781 ggcataaata aagcatttag ccaaatcaga atcacaacga catgtttaac ctgtggatcc
      841 tgggtgtaaa acgatgccat aaccccggca aagggagccg ttagccaggc gatggccgtt
      901 aatccaagag tggaaagcca gaacacatgc cgcaactgaa tctctgcttg cgctatctgc
      961 cctaccccca accttcggcc tgtaatgatc gtagaagcag agccgagcgc acttccgggt
     1021 aagttgataa gagccgcaat tgaaaacgcg ataaaatttc cggcaataac actggtcccc
     1081 atcccggcaa cgaacatttg ggttaataac cgaccactgg taaataacac tgattcgaca
     1141 ctcgcgggaa taccaatccc catgacttcc cagataatgc taaaattcag cggtttaaaa
     1201 tagctcttta acgaaatcct tagcgcagga ttaaaaccaa tcgccagcac ccacaaaatt
     1261 gcaactgcgc caatataacg agaaatggtt aaacccagcc ctgccccgac aaatcccagt
     1321 cccggccagg agaaaaggcc gtaaatcaat atgccgctaa taataatatt aagaatattc
     1381 aggctaccgt taatcaatag cggtattttc gtattccctg caccacgaag tgccccgcta
     1441 ccaataagag tgatggcagc tgctggataa ctgagtaccg tcagctccag ataagtcaac
     1501 gccagtgctt taacttctgt cgtggcatca cccgcgacga aatcaataat ttgttcgcca
     1561 aaatgatgaa taagcgttgc caacagtacg gcaaacaacg tcatgatcac caatgactgc
     1621 cgcgtcgcca ccctcgctcg tcgtcgatcc cgcttaccga gactaaatgc cacaacgaca
     1681 gtagtaccaa gatcgatagc agcaaaaaaa gccataatga ccatattgaa gctgtccgcc
     1741 aatcccacgc cggccatcgc atcttttccc agccagctga ccagaaaagt gctcagaacc
     1801 cccatcaaca ggacacaggc attctccatg aagataggaa cagcaagcgg ggttatctcg
     1861 cgccagaaca acactttgta gctcttgcgt ttagcgtgcc agcgagtgcc gtgaacaacc
     1921 tggcgtaaag cagaggagat attcaaagcc gaccttaatt gcagaaagtg aaaccacatt
     1981 tcaaataatg agggagaatc agcaaagctg caaagatttt cgccaacaaa ttgtctgcaa
     2041 atgcaacaaa ctgttgatag aaacggcaaa cagttgggga atttaaaaat cgggtttgac
     2101 aaaagatttt tcgccgttaa gatgtgcctc aacagcgatt cctctgtagt tcagtcggta
     2161 gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattt
     2221 ctgaaaagcc cgcttttata gcgggctttt tgctatatct gataatcaat ttcctcttca
     2281 ctgttttcca tcacctgccg cttgatatcc tcaactgaca gtcctgcatt acaaagttcc
     2341 agaaagcgcc agacatagtt acgctgaagt tgtcctcgct tcagtcccaa ccagacagta
     2401 ttagcatcga aaagatgccg cgtatccaga cggattaaat tctcttcctc ttgttcgccg
     2461 ctggattgtt cggcaactaa tccgatccca agcccaagag caacataggt tttaatgaca
     2521 tcagaatcct gcgcacttaa tacaatatct gccagcaaac ctttgcgggc aaatgcgtca
     2581 tcaatacgtg agcgccccgt aatcccctgt cggtaagtga ttaacggcca cttcgctatt
     2641 gattccagcg tcaatggtgt aatttgcgtt aagggatgat cgagtggaac aagcaaacta
     2701 tggtgccaac gaaaccacgg gaaggcgacg agctgcgggt cattactcaa acgctcgctg
     2761 gcgataccaa tatcagcttc gccattttgc aacaacgtcg caatttcctg tggcgtcccc
     2821 tggattagct cgagccgaac ctccgggaaa agttcgcgaa aagctttaat gacctctggc
     2881 aagctataac gtgcctgagt atgcgtcgtt gcaatagtga gaacgccaga cgtatcgttg
     2941 gtaaacaggt ctgcaagccg acgaacatta ctggcttcat tcagaatacg ttctgcaatg
     3001 accagtaatg ctttgcccgg ttcagtcatg cccagcagtc gcttacctcg tcgaacaaat
     3061 atttcgatgc caagttcatc ctccagttcc cgaatatgac ggctgacgcc ggactgtgag
     3121 gtaaaaagca tattcgcaac ctctgtcagg ttgtaatcct gacgtgcagc ctcgcggatt
     3181 atctttagtt gttggaaatt cacggtaaac tccgggcagt tcagatttcc cgttattgtt
     3241 aaagtctaat gcccggcata acaaataata aaaacccgca tcttattcca tcccgatata
     3301 acgcttagct caccaat
//
LOCUS       DAZPLI010000276         3229 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZPLI010000276 DAZPLI010000000
VERSION     DAZPLI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.279"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..495
                     /locus_tag="ACHUU3_004267"
     CDS             <1..495
                     /locus_tag="ACHUU3_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR2084157.1"
                     /translation="SRAPFIITPPLFRIEAGDDSSLRIIKTADNLPENKESLFYINVR
                     AIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRSDHSLNIYNP
                     TEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAINDYGGSSGTET
                     RPLQ"
     gene            525..3041
                     /locus_tag="ACHUU3_004268"
     CDS             525..3041
                     /locus_tag="ACHUU3_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR2084158.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3052..>3229
                     /locus_tag="ACHUU3_004269"
     CDS             3052..>3229
                     /locus_tag="ACHUU3_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2084159.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTRL"
BASE COUNT          941 a          773 c          734 g          781 t
ORIGIN      
        1 agccgtgccc catttattat aaccccaccg ctatttcgta ttgaagctgg cgatgactca
       61 tcactgcgaa ttattaaaac agctgataac ctgcctgaaa ataaagagtc gctgttctac
      121 attaatgttc gtgccattcc agcaaagaaa aaatcagatg atgttaatgc taacgagttg
      181 acgctggtat ttaaaacacg gatcaaaatg ttttatcgcc ccgcacacct gaagggacgg
      241 gtaaacgatg cgtggacatc actggaattt aaacgtagtg accattcact caatatatat
      301 aacccaactg aatattacgt cgtatttgcc ggactggcag tcgataaaac cgatctcaca
      361 agcaaaattg aatatatcgc gcccggagaa cataaacagt taccacttcc tgcatctggc
      421 ggaaagaacg tgaaatgggc tgcaatcaat gattatggcg gcagttccgg gacagaaact
      481 cgtccactgc aataacaaat ataaaaaaca caggtcatca gggaatgcca caacgacacc
      541 accagggaca taaacgcaca ccgaaacagt tggcgctcat catcaaacgt tgtttgccga
      601 tggtgctcac tggcagcggc atgctttgca ctaccgctaa cgccgaagag tattatttcg
      661 accccattat gctggaaact acaaaaagtg gtatgcaaac aaccgatctg tcacgttttt
      721 caaaaaaata cgcacaacta ccaggaactt atcaggttga tatctggctg aataaaaaga
      781 aggtttcaca gaaaaaaatt acatttaccg ccaatgcaga gcaacttctg cagccacagt
      841 ttacggtaga acaactacgt gagctgggta ttaaggtgga tgaaatcccg gcgctggctg
      901 aaaaagatga cgatagcgtg atcaactcgc ttgaacaaat cattcccggt acagcagctg
      961 aatttgattt caatcatcag cgacttaatt tgagcattcc ccaaattgca ctgtaccgtg
     1021 atgcaagagg ttacgtctcc ccttctcgtt gggacgatgg tataccaacg ctgtttacca
     1081 actactcgtt tacaggttct gataaccgtt accgccaggg caatcgtagc caacgacagt
     1141 acctgaatat gcaaaatggt gctaattttg gcccctggcg attacgcaac tattccacat
     1201 ggacacgcaa cgatcagaca tcaagctgga ataccatcag tagttattta caacgtgata
     1261 tcaaggcatt gaagtctcag ttgcttctgg gagaaagcgc caccagcggc agtatttttt
     1321 ccagctacac ctttactggc gttcaactcg cttccgacga taatatgttg ccaaacagcc
     1381 agcgcggatt tgccccaacg gtacgcggta tcgcaaacag tagtgcaatc gtgactatca
     1441 ggcaaaatgg ttatgtgatc tatcaaagca acgtgccagc gggtgccttt gaaattaacg
     1501 atctctaccc ctcttccaac agcggcgatt tagaagtcac gattgaagaa agtgacggta
     1561 cgcaacgtcg ctttatccag ccttattctt cattacccat gatgcagcga cctgggcatc
     1621 taaaatatag cgcgaccgct ggacgctatc gcgctgatgc aaacagtgat agcaaagaac
     1681 ccgaatttgc tgaagccacg gcaatatatg gtttgaataa tacttttacg ctgtatggtg
     1741 gcctgctcgg ttctgaagat tattatgcgc tggggatcgg tatcggcggc acacttggcg
     1801 cactgggcgc gttgtcgatg gatatcaaca gagctgacac ccaattcgat aaccagcact
     1861 cttttcatgg ctatcaatgg cgtacgcagt acatcaaaga tatcccggaa accaacacca
     1921 atatcgctgt cagctactat cgctatacca acgatggcta ttttagtttt gatgaagcca
     1981 atacccgtaa ttggaactat aacagtcgcc aaaaaagtga aattcaattc aacatcagcc
     2041 agacaatatt tgatggggta agtctgtatg cctccggttc gcagcaagac tattggggca
     2101 ataacgataa aaacaggaat atctcagttg gggtttccgg tcagcaatgg ggaattggtt
     2161 acagcctgaa ttatcaatac agtcgctaca cggatcaaaa taatgaccgc gcactctctt
     2221 tgaatctcag tattccgtta gaacgctggt taccgcgtag ccgggtttcc tatcagatga
     2281 ccagccagaa agatcgccca acccaacatg aaatgcgtct taatggctca ctgctggatg
     2341 atggtcgcct gagctatagc ctggaacaaa gtctggatga cgataacaac cataacagta
     2401 gcctgaacgc cagttaccgt tcaccttatg gcaccttcag tgccggatac agctacggta
     2461 atgacagcag ccaatacaat tacggcgtta ccggcggcgt ggttatccat actcatggcg
     2521 tgacgctctc gcaatatctg ggcaacgctt ttgcgcttat cgatgctaat ggggcttctg
     2581 gcgtgaggat acaaaactat ccggggattg ctactgatcc ctttggctat gcagtggttc
     2641 cttatctcac gacttaccag gaaaaccgtc tctcggtaga tactacgcag ctgcccgata
     2701 acgtcgatct tgagcaaaca acacagtttg tggtgcccaa cagaggtgca atggtagcgg
     2761 cgcgtttcaa cgccaatatc ggttatcgcg tacttgttac agtcagcgat cgcaacggta
     2821 aaccgttgcc ctttggcgct cttgccagca acgatgagac ggggcaacaa agtatcgtcg
     2881 atgagggcgg catactatat ctctctggga tatcgagtaa atcacaaagc tggactgtac
     2941 gctggggaaa tcaggcagat caacaatgtc agtttgcttt tagtacaccg gattcagaac
     3001 caacaacctc tgtattacaa ggcacggcgc agtgccatta aggataaaaa aatgaaaaga
     3061 gcgcctctta taacaggact tttgttgata tccacatcct gcgcttatgc ctcctcagga
     3121 gggtgtggag ctgacagcac tagcggtgcg acaaattaca gcagtgtggt tgatgatgtt
     3181 acggtgaacc agacagataa cgtgacagga cgggagttta ctagactgg
//
LOCUS       DAZPLI010000277         3185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZPLI010000277 DAZPLI010000000
VERSION     DAZPLI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.280"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(9..635)
                     /gene="dsbA"
                     /locus_tag="ACHUU3_004270"
     CDS             complement(9..635)
                     /gene="dsbA"
                     /locus_tag="ACHUU3_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR2084160.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(652..1638)
                     /gene="srkA"
                     /locus_tag="ACHUU3_004271"
     CDS             complement(652..1638)
                     /gene="srkA"
                     /locus_tag="ACHUU3_004271"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR2084161.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1715..1984)
                     /locus_tag="ACHUU3_004272"
     CDS             complement(1715..1984)
                     /locus_tag="ACHUU3_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR2084162.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2054..2638
                     /gene="mobA"
                     /locus_tag="ACHUU3_004273"
     CDS             2054..2638
                     /gene="mobA"
                     /locus_tag="ACHUU3_004273"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR2084163.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2620..3147
                     /gene="mobB"
                     /locus_tag="ACHUU3_004274"
     CDS             2620..3147
                     /gene="mobB"
                     /locus_tag="ACHUU3_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR2084164.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          832 a          772 c          769 g          812 t
ORIGIN      
        1 acattgaatt attttttctc ggacagatat ttcactgtat cagcatactg ctgaacaaaa
       61 acatccatat tgctggtatc cataccctgc ggattcagct gatatttacc gttaacaaac
      121 atcgccggaa cgccacgcaa ttgcacgtca gctgcagctt tttcctgctg agcgaccaga
      181 gatttgacca cgaagctgtt ccacgccgcg tcgtactctt cacctttaat acctgcgttg
      241 ataaatacat cgcggatatc agatgctgaa cgaatggtct gggttttctg tacgccttca
      301 aacagcggaa cagtgacttt gtcttccacg cccagcgcca tcgccacagc ccatgcctga
      361 gtcagatctt tgcccaggtc accacccatg aagttgacgt ggtatttagt catcttcacg
      421 ccttccggca gttttttctt cacattatca gaaatatgca gaacttcttc aaactgatag
      481 cagtgcggac agaagaaaga gaaaaactcc agcacttgcg gcgcgccagc taccggtttt
      541 tccagggtag tgtactgttt accatcttca tactgcgccg ccgatgcgct aaacgctaaa
      601 actaaaccag ccagcgccag ccaaatcttt ttcatgatct actctctccg attaatacat
      661 aggtgttaat tgtaaagggg gttcttgtag aacttttgcc tgttctataa aagtcgccgt
      721 ttgtcgcagc cagtaatctt ccccggttaa ccacgggaaa tttttcggga acgcgggatc
      781 agcccaacgc cgcattagcc aggcaagata ataaaccaaa cgcatggcgc gtaaaggttc
      841 aatcagtccg atttcagcgg tgtcgaactc gccaaattct tcataagctt caataatagt
      901 ttccagttgc atccgctgct cggctttatc gccattgagc aacatccaca aatcctgaat
      961 agctggacca ttacgtgcat catccagatc aacaaacatt ggaccatcgc gccagagaat
     1021 attcccggcg tggcagtctc catgtagccg cagaacggtg aaatcttccc gccagtgtgc
     1081 tgtaacggcg gcgatcagct catctgtcgc tttcaggaat gccgctttca acccggaagg
     1141 tatcagtgta gcgtcctcaa acagcttgcg tggctcaatg agatattcat tcagaccgat
     1201 ggtcgggcga tggataaaaa gctgtttgtg ccccgtctgg tgcatacgcc ctaaataacg
     1261 cccaaccgct tccgtctgat cgatattatc agcttcgaac tggcgaccac cgacgcttgg
     1321 aaaaacagcg aaataaaatc cctgatgatt caataaagtc tgaccgttaa aggccacagg
     1381 tgctgcgacc ggaacttcat catttaccag ctgcaacgca aattgatgtt cttcgaggat
     1441 ttgatcggct gtccaacgtt cagggcgata aaatttgacg acaaaacgtc gacgctcttc
     1501 gtcctgaaat tgatagacac ggttttcata gctgttaagc ggggtaagac cggaatccac
     1561 ccggatccct tgctcaaaca gagcgtccat gatggtatcc gggtgtagtg tctggaaagt
     1621 aaaagcgctg ttgttcatcc catcatccgg aaaacattac gaatgatgaa ggatatcatt
     1681 tcacgatgat tttggtggcg ccgcttacaa gcttttactc tttaattacg ccgcgagcgc
     1741 gtaacagcgc cgttttgaag tcttcttcat aatctttctg caaacccggg atcaccgcat
     1801 ctttcgcgga atcacgcatt ttcaggtgat agatcagaat gtcatccgtc aaatccgcca
     1861 gttcgccgtc aaaacctgac tctttcgcca gtttctgcaa aaattgcaac aggttaaggt
     1921 ctggctcttt ttgccaggct ggctggagga gttcaataac ttcattcaga cgtttacatt
     1981 tcatagtgat gctccttact cttgagacag acacgttagc agggtcaatc ccacaataaa
     2041 agaggcgata tcggtgaatc tgatgacgac gataacaggc gttgtgctgg caggcggtaa
     2101 agccagacga atgggcggcg tagataaagg attgcttgaa ttaaacggca aaccattatg
     2161 gcaacatgtc gctgacgcgc ttatgacgca gctctctcac gtcgtggtta atgccaatcg
     2221 tcatcaggaa atctatcagg caagcggtct gaaagtgatt gaagattcac tggcggatta
     2281 cccaggccct ctggcaggaa tgctttcagt aatgcagcag gaagctggtg agtggttttt
     2341 gttttgtccg tgcgatacgc cttacattcc ccatgtttta gcttcccggc ttaatcatca
     2401 gcgcaaagat gcgcctgtcg tgtgggtcca tgacggtgaa cgcgatcacc cgactattgc
     2461 tctggtaaac cgtgctattg agcctttatt actagaatat ctgcaagcag gagaacgccg
     2521 ggtaatggca tttatgcgtc tggctggcgg ccatgcggtt gatttcagcg atcataaaga
     2581 tgcatttgtt aacgtgaata cgccagagga gcttgcccga tggcaggaaa aacgatgata
     2641 ccgttactcg cctttgccgc gtggagtggt accggaaaaa ctacgctttt gaaaaaactg
     2701 atcccggcat tatgcgccag agggatccgt ccagggctga ttaagcatac gcaccatgat
     2761 atggatgttg ataagccagg caaagatagc tatgagctgc gcaaggctgg cgcggcacaa
     2821 accatcgttg ccagccagca acgatgggcc ttgatgacgg aaaaaccaga cgaagaagag
     2881 ctggatctac agtttctcgc aagtcgaatg gatacctcaa agctggattt gattctggtc
     2941 gaagggttta agcatgaaga gatcgcaaag attgtgctgt ttcgcgatgg agccggacat
     3001 cgacctgaag aattagtgat agacaggcat gttattgctg tagccagtga tgtgccgctt
     3061 aatcttgatg tcgcattact ggatattaat gatgttgagg ggctggctga ttttgtggtg
     3121 gagtggatgc aaaagcagaa cggataagca agagtaggct ggataagacg cgccagtgtc
     3181 gcatc
//
LOCUS       DAZPLI010000278         3181 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZPLI010000278 DAZPLI010000000
VERSION     DAZPLI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.281"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHUU3_004275"
     CDS             <1..122
                     /locus_tag="ACHUU3_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2084165.1"
                     /translation="GTVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            456..2735
                     /gene="ydeP"
                     /locus_tag="ACHUU3_004276"
     CDS             456..2735
                     /gene="ydeP"
                     /locus_tag="ACHUU3_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR2084166.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2983..3180
                     /gene="safA"
                     /locus_tag="ACHUU3_004277"
     CDS             2983..3180
                     /gene="safA"
                     /locus_tag="ACHUU3_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR2084167.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
BASE COUNT          874 a          717 c          750 g          840 t
ORIGIN      
        1 tggggacagt aaataaaagc aaagtacctc tgggtttaag cgcaacctat ggacaaacag
       61 gaaacaaggt ttcagccgga accgtccagt cagtcattgg tgtcactttt atctatgaat
      121 aattgtgatt ttgaccttag tctatgcgct ccattcacga aaattgcgca gataattatt
      181 ttgtgaaggc tattatccta cacctgtaag aaaatccgcg cacaattgaa gccagagtct
      241 tattttttat attgaaaaat atgttaattt aaacactatg agagatgatg cttaacttat
      301 cgtatttaat ctattgttta acgataatag attaaaaata gaaagagagt agtcccctac
      361 tcttatatat ccatgttggc gataatccct ttgtatgtac tgtgcatcat cgctattaca
      421 aatcctaata attcatttcc acacaggata agtagatgaa gaaaaaaatt gaatcctacc
      481 agggtgctgc aggtggttgg ggtgctgtta aatccgtagc gaatgcagta cgtaagcaga
      541 tggatatacg ccatgatgtt attgccatgt ttgacatgaa taagccagag ggctttgact
      601 gtccgggttg tgcatggcca gatcctaagc acagtgcgtc attcgacatt tgtgaaaacg
      661 gcgcaaaagc aatcgcctgg gaagtcacgg ataagcaggt aaatgcctct ttctttgctg
      721 agaatacggt tcaatcatta cttacctggg gagatcacga gcttgaggct gcagggcgtc
      781 tcactcagcc tttgaaatat gatgccgtca gcgactgtta caagccatta agctggcaac
      841 aagctttcga cgaaattggc gcacgccttc aaagctatag tgatcccaat caggttgaat
      901 tctatacttc gggccgcact tccaatgaag ctgcctttct ttatcagctt tttgcccgtg
      961 aatacgggag caataacttt cccgactgct ccaacatgtg ccatgaaccg acaagcgtgg
     1021 gtttggcagc gagtatcggt gtaggtaaag ggaccgtgtt gctggaagac tttgagaagt
     1081 gcgatttagt catttgcatt gggcataacc ctggtacaaa ccaccctcgc atgctgactt
     1141 cgttgcgcgc tttagtgaaa cggggagcga aaatgatcgc catcaatcct ctacaggaac
     1201 gtggcctgga gcgatttacc gcaccgcaaa acccgtttga aatgctgacg aactctgaga
     1261 ctcagttggc cagtgcctac tacaacgtgc gcattggtgg cgatatggcg ttgctcaagg
     1321 ggatgatgcg cctgttaatt gagcgcgatg atgctgcaag tgccgcaggt cggccctcat
     1381 tgcttgatga cgaatttatt caaacgcata ccgtcggctt tgacgagcta cgccgtgacg
     1441 ttctcaattc cgagtggaaa gatatcgaac gtatttctgg actaagtcag acacaaatcg
     1501 ccgaactggc tgacgcatat gccgctgccg atcgcaccat tatctgttac ggaatgggga
     1561 tcactcagca cgaacatggt acccagaatg tacagcaact ggtcaatctg ctgttgatga
     1621 aaggtaacat tggcaagcct ggtgcgggta tctgcccact acgaggtcac tctaatgtac
     1681 agggcgaccg aaccgtcggt atcaccgaga aaccgtctgc agagtttctg gctcgcctgg
     1741 gtgagcgcta tggcttcacc ccacctcatg cacctggaca tgctgcaatt gccagcatgc
     1801 aagcaatatg cacggggcag gctcgagcat tgatctgcat ggggggcaac tttgcactgg
     1861 caatgccaga tcgggaagcg agcgctgtac cgttaacgca attagatttg gcggtacacg
     1921 tagccactaa gcttaaccgc tctcatctgc tgaccgcacg gcatagctat attctgccgg
     1981 ttctggggcg tagcgagatt gacatgcaaa aaagcggtgc gcaggcggta accgttgagg
     2041 attcaatgtc gatgattcat gcctcgcgtg gcgtgttaaa acccgccggt gtaatgctga
     2101 aatcagagtg tgcagtggtc gcgggaatcg cgcaggcagc actaccccac agcgtggtag
     2161 cctgggagta tctggtggaa gattatgatc gcattcgcaa tgacattgaa gctgtgttac
     2221 cagagttcgc cgactataac cagcgcatcc gacatcctgg aggttttcac ctgataaatg
     2281 cagctgctga aaggcgctgg atgacgccgt caggtaaggc taatttcatt accagcaaag
     2341 ggctgttaga agatccctct tcagcgttta acagtaagct ggtcatggcg acagtacgta
     2401 gccacgatca gtacaacacg acgatttatg gtatggatga tcgctatcga ggggtattcg
     2461 gtcaacgaga tgtggtcttt atgagtgcta aacaagctaa aatttgccgt gtaaaaaacg
     2521 gcgaaagagt taatcttatt gcgcttacgc cagacggtaa gcgcagctca cgccgcatgg
     2581 atagattaaa agtggtcatt taccctatgg ctgaccgctc actggtgacc tattttccag
     2641 aatcgaatca catgctaact cttgataacc acgatccatt aagtggcatt cctggttata
     2701 aaagtattcc ggttgaatta gaaccatcaa attaattcgc ctcctcattt cttctgctgt
     2761 catccgcaca gcagaagaat ttctcattga ctattatcgc gcaatttgct cacattgatt
     2821 aaattaaacc acacactata agatataaac ctctgcctac agctgtaaga aactccgctc
     2881 agtactgaag tgccagtcct atttcctctt ttctccagcc tgttatatta agcatactga
     2941 ttaacgattt ttaacgttat ccgctaaata aacatatttg aaatgtatgc gaccacagtg
     3001 aaaaacaaaa tcacgcaaag agacaactat aaagaaatca tgtctgtaat tgtggttgtc
     3061 ttattactga cccttacgtt gatagccatt ttttcggcaa ttgatcagct gggtatttca
     3121 gaaatgggtc gcattgcaag agatcttaca catttcatta tcaatagttt gcaagactga
     3181 a
//
LOCUS       DAZPLI010000279         3155 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZPLI010000279 DAZPLI010000000
VERSION     DAZPLI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.282"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..148
                     /gene="ynaL"
                     /locus_tag="ACHUU3_004278"
     CDS             <1..148
                     /gene="ynaL"
                     /locus_tag="ACHUU3_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR2084168.1"
                     /translation="PVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKLSHRERRSAR
                     IRAC"
     gene            178..1551
                     /gene="dbpA"
                     /locus_tag="ACHUU3_004279"
     CDS             178..1551
                     /gene="dbpA"
                     /locus_tag="ACHUU3_004279"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR2084169.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(1680..2615)
                     /gene="ttcA"
                     /locus_tag="ACHUU3_004280"
     CDS             complement(1680..2615)
                     /gene="ttcA"
                     /locus_tag="ACHUU3_004280"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR2084170.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2667..>3155)
                     /locus_tag="ACHUU3_004281"
     CDS             complement(2667..>3155)
                     /locus_tag="ACHUU3_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR2084171.1"
                     /translation="VNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGH
                     HRKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRR
                     RNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNAR
                     LS"
BASE COUNT          728 a          796 c          797 g          834 t
ORIGIN      
        1 tcctgtccct gaaccgattc ctggcgatcc tgttccagtg cccgatccga tccctcgccc
       61 gcaacccatg cctgacccac cacccgatga agaaccgatt aaattgtcgc atcgtgagcg
      121 tagatctgcg aggatacgcg cctgctaact ttgcgtcgat gaccacgaga atagattgtg
      181 accgcttttt ctaccctgaa tgttttgcct cccgcccaac tcacgaacct taatgagttg
      241 ggttatttaa ctatgacgcc ggtgcaggcc gccgcgcttc cggcgatcct tgccggaaaa
      301 gatgttcgcg tgcaggcgaa aaccggcagc ggcaaaacgg cggcttttgg cctcggcttg
      361 ttacagcaaa ttgatgcgtc gctatttcaa acccaggctt tagtgctgtg tcctacgcgt
      421 gaactggcgg atcaggtggc aggtgaattg cgtcggctgg cgcgttttct gccaaatacc
      481 aaaattttga cgttgtgcgg tggtcaaccg ttcggtatgc agcgtgattc gttgcaacat
      541 gcgccgcata ttatcgtggc aacgccgggt cgtttgctgg atcacttgca aaaaggcacg
      601 gtatcactgg atgcgctgaa tactttggtg atggatgagg ccgaccgcat gctggatatg
      661 ggatttagcg atgccattga tgatgtcatc cgttttgcgc ctgcatctcg acagacgctt
      721 ctgttttcgg caacctggcc ggaagccatt gccgtaatca gcggacgagt gcaacgcgat
      781 cctttggcga ttgaaattga ctcaacagat gctttgccac ccattgaaca acaattttat
      841 gagacatcca gcaaaggcaa aattcctctg ttgcaacggt tattaagctt gcatcagcca
      901 tcctcttgcg tggtgttttg caataccaaa aaagattgcc aggctgtctg cgacgcgctg
      961 aatgaagtag ggcaaagtgc attgtcatta cacggcgatt tggagcaacg cgatcgcgat
     1021 cagaccctgg tacgttttgc taacggtagc gcccgtgtac tggttgcgac tgatgttgct
     1081 gcgcgtggtc tggatattaa atcgcttgag ctggtggtga actttgagct ggcgtgggac
     1141 cctgaagttc atgtacatcg catcggccgt acagctcgtg caggaaatag cggtctggcg
     1201 atcagtttct gtgctccgga agaagcacag cgggccaata tcatttctga catgttgcag
     1261 ataaaactta actggcaaac gccgccagct aatagttcca ttgtgccgct ggaagcagaa
     1321 atggcaacgt tgtgtatcga tggtgggaaa aaagccaaaa tgcgcccggg tgatgtatta
     1381 ggtgcactga caggagatat cgggcttgat ggcgcagata ttggcaaaat cgccgtgcat
     1441 ccggcgcatg tctatgtcgc ggtccgtcag gctgttgctc ataaagcatg gaaacagtta
     1501 cagggcggga agattaaagg aaaaacgagc cgggtgcggt tattaaaata atgaaatgtt
     1561 gaattgccgg gtgcaagagt aaacatctta ttcgggattg ccggatgcga cgctggccgc
     1621 gtcttatccg gcctccataa gagtagcccg atacgcttgc gcatcgggcg ccatcctggt
     1681 tatttcactt caaccacatt cagccgtaac tcatccaact gattttcatc ttcttctggc
     1741 tgccagcccg ccggttgtag tgggatctct tcgcgatcaa acgccagatc acccccgtta
     1801 accacttcag aaccgtgggt gatgcctttg aaatcgaaca ggttggtatc gcacagatgc
     1861 gacggcacca cattctgcat cgcgctgaac atcgtctcga tacgccctgg ataacgttta
     1921 tcccagtcac gcaacatgtc agcaatcacc tgacgttgca ggttaggctg tgaaccgcac
     1981 aggttgcacg gaataatcgg gaacgctttt gcatcggcaa atcgctgaat atctttctcg
     2041 cggcagtagg ccagcggacg aataacgata tgtttgccat catcgctcat cagtttcgga
     2101 ggcatacctt tcatcttgcc gccgtagaac atatttaaga acagcgtctg caggatatcg
     2161 tcacgatggt gacccagcgc gatcttcgtc gcccccagtt ccgttgcggt acgataaagg
     2221 ataccgcgac gaaggcgaga acacagtgag caagtggttt tgccctctgg aatcttctct
     2281 ttcacgatac cgtaagtgtt ctcttcgaca atcttgtact caacgcccag cttttcaaga
     2341 tactcgggca gaacgtgttc cgggaagccc ggttgctttt gatcgaggtt aacagccacc
     2401 agcgaaaaat tgattggcgc gctttgctgc aaattgcgca gaatctccag catggtatag
     2461 ctgtctttac ccccggagag gcaaaccatg atgcgatcgc cttcttcaat catattgaag
     2521 tcagcaatgg cttcgcccac gttacgacgc agacgttttt gtaatttgtt caggttgtat
     2581 tgttctttct ttgtaatttg ttgattttct tgcattattt cagttctctg gtactaaatg
     2641 gggcaaattg ggggcaaact ttgcaactac gataaccgcg cattcaacat ggctatctgt
     2701 tcgtcgttca tgtcatcaat ccacataccg taaatttcat acaccatttg cgcagtttca
     2761 tgccccatct ggctggctat aaatgccggg ttcgctcctg ccgtcaacag ccagcaggca
     2821 aaagtatgtc gcgtatggta cggattacgg cggcgaatac cagcacgttt tactgctgca
     2881 ttccatcttg cacccaaact gcttaccgag taataaggtt tctgtttttc gttacacacc
     2941 ctgggcatga aaacaaaatg cagtttttgc ttttcagttc tgccgtactc ccgatgataa
     3001 aaagtgattt cgcttttgcg atgatgcccg gtcagtttgt attgctcctt cagtgcttca
     3061 agagccggct gcagtaatgt taccgtccgg atcccggcat ttgtttttgg gggaccgaac
     3121 atatcaagta tcgtcaggtt tcttctgaca ttcac
//
LOCUS       DAZPLI010000280         3046 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZPLI010000280 DAZPLI010000000
VERSION     DAZPLI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.283"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..173
                     /locus_tag="ACHUU3_004282"
     CDS             <1..173
                     /locus_tag="ACHUU3_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084172.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            186..1037
                     /gene="hyi"
                     /locus_tag="ACHUU3_004283"
     CDS             186..1037
                     /gene="hyi"
                     /locus_tag="ACHUU3_004283"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFR2084173.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            956..2134
                     /gene="allB"
                     /locus_tag="ACHUU3_004284"
                     /pseudo
     CDS             956..2134
                     /gene="allB"
                     /locus_tag="ACHUU3_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2191..>3046
                     /locus_tag="ACHUU3_004285"
     CDS             2191..>3046
                     /locus_tag="ACHUU3_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFR2084174.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          696 a          710 c          836 g          804 t
ORIGIN      
        1 aggcgaaagc cttaatggcg caatatcggg taccggtagt cgtggaagtt attcttgagc
       61 gtgtgaccaa tatttcgatg ggcagcgaac tggataacgt catggaattt gaagatatcg
      121 ccgataacgc agtggacgca ccgactgaaa cctgcttcat gcactatgaa taagggagat
      181 aaataatgtt acgtttctct gctaatttat cgatgttatt tggtgaatat gattttctcg
      241 cccgttttga gaaagctgcg cagtgtggtt ttcgcggcgt tgaatttatg tttccttatg
      301 actacgacat tgaagaatta aaacaggtgc tggcgagtaa taaactcgaa catacgctgc
      361 acaatctacc ggcgggtgac tgggcggcgg gggagcgcgg tattgcctgt attcctggcc
      421 gtgaagaaga gtttcgggat ggcgtagcag cagcgattcg ttatgcccgt gcgctgggga
      481 ataaaaaaat taactgtctg gtcggtaaaa cgccggctgg tttcagcagt gaacagattc
      541 acgcaacgct tgtagaaaac ctgcgttatg ccgcgaatat gctgatgaaa gaagatattt
      601 tattactgat tgaacctatt aaccattttg atattcctgg tttccatctc accggaactc
      661 ggcaggcgct gaaattgatt gatgatgttg gttgctgcaa tttgaaaatt cagtatgaca
      721 tttatcacat gcagcggatg gaaggtgaat taaccaacac catgactcag tgggccgata
      781 aaattggtca cctgcaaatt gctgataatc cgcatcgcgg cgaaccgggg accggagaaa
      841 ttaattatga ttatctcttt aaggtaatcg aaaattctga ctacaacggt tgggttgggt
      901 gtgaatataa accccaaacc accacggaag ccggtttacg ctggatggat ccgtactttc
      961 tgaaccgggt cgtagccact gggaaggtta tgaaaccggt actcgcgcag cggcaaaagg
     1021 tggtatcacc accatgatcg aaatgccgct caaccagctg cctgcaacgg ttgaccgcgc
     1081 ttcaattgaa ctgaagttcg atgccgctaa aggcaagctg actatcgatg cggcacaact
     1141 cggtggcctg gtgtcttaca acatcgaccg tctgcatgag ctggatgaag tgggcgttgt
     1201 cggcttcaaa tgcttcgttg cgacctgtgg cgatcgcggt atcgacaacg acttccgtga
     1261 tgtaaacgac tggcagttct tcaaaggtgc gcagaagctg ggcgaactgg ggcagccggt
     1321 gttggtgcac tgcgaaaacg cgctgatctg tgacgcactg ggcgaagaag cgaaacgtga
     1381 aggtcgcgta actgcccatg actatgtggc ttcgcgtccg gtatttaccg aagtggaagc
     1441 gattcgccgc gtgctgtatc tggcgaaagt tgccggttgc cgtctgcacg tttgccatat
     1501 cagcagcccg gaaagtgttg aagaagtgac tcgtgcacgt caggaaggtc aggatgttac
     1561 ctgtgaatcc tgcccgcatt actttgtact ggataccgat cagttcgaag aaatcggtac
     1621 tctggcgaag tgttcaccgc cgatccgcga tctggaaaac cagaaaggca tgtgggaaaa
     1681 actgtttaac ggtgaaatcg actgcctggt ttccgaccac tcaccatgcc ctccggaaat
     1741 gaaagccggc aacatcatgg aagcatgggg cggtattgcc ggtctgcaaa actgtatgga
     1801 cgtgatgttc gatgaagcgg tacagaaacg cggaatgtct ctgccaatgt tcggcaaatt
     1861 aatggcgact aacgcagcag atattttcgg tctgcagcaa aaaggccgta tcgccccagg
     1921 aaaagatgcc gacttcgtct tcattcagcc gaatagcagc tatgttctta ccaatgacga
     1981 tctggaatat cgccacaaag tcagcccgta tgttggccgt accattggcg cgcgtatcac
     2041 gaaaaccatc ttacgtggtg atgtgattta cgacattgaa cagggcttcc ctgttgcgcc
     2101 gaaaggtcaa tttatcctta aacatcagca gtaatctggc ccctgcaatg cccgtccttg
     2161 cggcgggcat tctccggtta aggtgtgttt atgttcaatt ttgcagtcag ccgcgaaagc
     2221 ctgttatcag gatttcagtg gtttttcttt attttttgca acacggttgt ggttcctcct
     2281 acgctacttt ctgcttttca gttgccgcaa agtagcctgc ttacgctcac gcaatatgct
     2341 tttcttgcta ccgcactggc ctgcttcgct caggcgttct gcggtcatcg tcgcgctatt
     2401 atggaagggc cgggtggcct gtggtgggga accatcctta ctatcaccct tggcgaagca
     2461 tcgcgcggga caccgatcaa cgatatcgct accagtctgg cggtggggat tgcactctcc
     2521 ggcgtgctga cgatgttgat tggttttagc ggattaggcc atcgcctggc acggttattt
     2581 acgccgtcgg tgatggtctt gtttatgttg atgctgggcg cacagctgac cactatcttt
     2641 ttcaaaggta tgctcgggct gccgtttggc atagccgacc catattttaa aattcagtta
     2701 ccaccgttcg cgctctcggt ggcggtgatg tgcctggtac tggcgatgat tatctttctg
     2761 ccgcaacgtt ttgcccgtta tggcctgctg gtcggcacca taaccggctg gttgttgtgg
     2821 tacttttgct ttccttcttc gcattcgctc tccggtgagt tgcactggca gtggttcccg
     2881 ctcggcagtg gcggtgcttt gtcgccggga attattctga cggcggtgat tacaggtctg
     2941 gtaaatatca gcaataccta cggtgcgatt cggggcacgg atgtttttta tccgcagcag
     3001 ggcgcgggga atacgcgtta tcgtcgcagc tttgtggcga ccggat
//
LOCUS       DAZPLI010000281         2992 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZPLI010000281 DAZPLI010000000
VERSION     DAZPLI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.284"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..1076
                     /gene="bglB"
                     /locus_tag="ACHUU3_004286"
     CDS             <1..1076
                     /gene="bglB"
                     /locus_tag="ACHUU3_004286"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR2084175.1"
                     /translation="RLFDEMAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEH
                     YARTVFTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARA
                     VKACHSLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYM
                     QRFFRDHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPN
                     PHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIA
                     YLNDHLVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLT
                     RTRKKSFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="ACHUU3_004287"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="ACHUU3_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFR2084176.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2992
                     /locus_tag="ACHUU3_004288"
     CDS             2805..>2992
                     /locus_tag="ACHUU3_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HFR2084177.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          838 a          632 c          744 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc tt
//
LOCUS       DAZPLI010000282         2938 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZPLI010000282 DAZPLI010000000
VERSION     DAZPLI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.285"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            37..264
                     /locus_tag="ACHUU3_004289"
     CDS             37..264
                     /locus_tag="ACHUU3_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR2084178.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(265..639)
                     /locus_tag="ACHUU3_004290"
     CDS             complement(265..639)
                     /locus_tag="ACHUU3_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFR2084179.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHUU3_004291"
                     /pseudo
     CDS             complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHUU3_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2099..2674)
                     /locus_tag="ACHUU3_004292"
     CDS             complement(2099..2674)
                     /locus_tag="ACHUU3_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR2084180.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          761 a          695 c          689 g          793 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc atcattagcc gcactatacc ggggcagggg
      121 cacggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ttggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 actcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg cagcgtgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcggatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg tccggcgatt tggttgctcg agttataaac taacagtgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgcc gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga tcggctggaa tttttctctg atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggcggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ctaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctca tatatgctat cttaatgcac tacctgataa
     1801 gtgagcctat acaggttatt actgtatgtt tatacagtat tattggtaga agactgtgta
     1861 gatttggtat agagatgaat agatactatt aaggtaactt attgaaatca ataaagattc
     1921 ttgtgcagtc tgaatagata acttagcgtt atgtctagtc gtttccctct ccattcctaa
     1981 aaagtgctgt atactggcct atacaacgct ataaagacct ctacatagcg ccttgatagc
     2041 tatgtcacag gtacaaaaaa gccccctata aaggaggctg tgtagtctat taagggtctt
     2101 acagggtctt ggtccagtgt ctgctaacag tccttacaga gaggttaaga accttagcta
     2161 cctgagactg agatagtccc ctttccttgt actctaagac cgctctagtt gtgtcactcg
     2221 ctataggtct tccaagtacc ttaccttgaa ctctagcacg ctctagaccc tgtttagtgc
     2281 gctctctgat acggtttctt tcaaactctg caaaggtact aaatagctgt agaaccaact
     2341 taccctctga gcttgaaagg tctttaacgg gtaaatctaa acagatgagc ttcacaccct
     2401 tatcattaag aagggtcact gtattctgta ggtcgatgtt atcccttccc agacggtcaa
     2461 gtttaagaac aacaagggtg tcaccttctt ctagcttatg gttgaccaga ttcgcaaatc
     2521 cgtccctatc catagctgac actgaaccag agacaacctc ctctataact cgtgacttag
     2581 gaatatcata gccagccttc ttaatggctt gaatctggtt ctctgtggtg gaagtctcta
     2641 ctgatacacg gcagtaagca aaggttcgtg acatagatat ccctctgggt agtcattaaa
     2701 cggttagttt gtcttgtcta tagacattaa agcaaatggt aggtttgttg tctatagcta
     2761 tagtgactct tctatgtaga tattaaacgg acgtttcttg tcgtgggtca catagatact
     2821 tttagggctt ctgtcaagtg atattcgtag attttgcaag ggggaggtgt aaggctgaaa
     2881 cagggctact aagaacacat acatctaagg ctggaaagta cctgagcaga tattcttc
//
LOCUS       DAZPLI010000283         2917 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZPLI010000283 DAZPLI010000000
VERSION     DAZPLI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.286"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..493
                     /locus_tag="ACHUU3_004293"
     CDS             <1..493
                     /locus_tag="ACHUU3_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR2084181.1"
                     /translation="ADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPAP
                     DESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALM
                     LRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPA
                     RSK"
     gene            493..1083
                     /locus_tag="ACHUU3_004294"
     CDS             493..1083
                     /locus_tag="ACHUU3_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFR2084182.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1832..2110
                     /locus_tag="ACHUU3_004295"
                     /pseudo
     CDS             1832..2110
                     /locus_tag="ACHUU3_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            2170..2658
                     /locus_tag="ACHUU3_004296"
     CDS             2170..2658
                     /locus_tag="ACHUU3_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR2084183.1"
                     /translation="MTKQYDVTWLFSEDGKNWYEEQKNFASDTIKMVYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVS
                     ARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFSTITDKSSYNAIEWSVSPEG
                     SS"
     gene            2682..>2917
                     /locus_tag="ACHUU3_004297"
     CDS             2682..>2917
                     /locus_tag="ACHUU3_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR2084184.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVY"
BASE COUNT          759 a          636 c          831 g          691 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga
      121 acgcccggcc ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc
      181 acctgttgcc ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac
      241 gattgcatta tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc
      301 actgatgtta cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc
      361 catcagtttg tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga
      421 cctgggggag accgagcttc cggtgctcgg cgagatcacc tggcaggctt atgacccggc
      481 aaggagtaaa taatggattc gttacaggat gattatacaa agctgctgta cggactgatg
      541 ccgccggggc ctgcatggtc agataccgac ggtgtacttg acggtctggc accatcgctt
      601 gtgcgtgttc accagcgggc tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca
      661 gagctgattg aacgttatga agaattgtat ggtttacctg attcctgttc ccctgtcgga
      721 acccagacat tacgccagcg tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc
      781 ataaatgagc agttcttcct ggatcagctt gaggcgctgg gatataccgg cgtaacgatc
      841 gaacagttcc agcacttgga tgcaagcccc gatccggaat ggggagatcg ctggcgttat
      901 ttctggcgtg tgacgttgcc gatggatgcc ggtgctcagt ggcagacatg cacggacgcc
      961 tgcaacacac cgatccggac gtggggggat acggttgcgg agtgcgtgat taataaatta
     1021 tgtccgtcac acaccgtcgt tttattttcc tatccagatg aggatgaaga tgcacaggat
     1081 tgatacgctg actgcggtaa aagataagtt tggtccgggt aagaacggat ttactgacgg
     1141 aaatcttcgc acaggacgtc ttgctaccag gctgaacagt gccatgtgga atgccattca
     1201 ggaggaaatc tgtggtgtca ttgagaaggc cgggatagaa ctgaataaag aagaacacga
     1261 ccagctatat aaggctatat tattgctggt gggcggtgca attaacgaag aggcattgct
     1321 gataaaaaat aacctttcgg atgtggaaga cagggatgag gctgttgaaa acctcggatt
     1381 aaaacccacg gtggataagg caaaaaatgc cgttcagcgt gatggcgaca ccatgaccgg
     1441 agagctgaaa atccgtggtg ttaatgcgct gaggattttc aacgaagctt ttggcctgat
     1501 ttttcgtcgt tcggaagagt gcctgcacct tattcccacc agtgaaggtc agggggaaaa
     1561 tggcgatatt ggtcccctgc gcccgttcac cattaatctg cggacgggtg aaatatccat
     1621 gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt
     1681 tcagaacgcc ctggggggga attcaattgt tcttggtgat aatgacaccg gattcaaaca
     1741 gaatggtgat ggcctgctgg atgtttatgc caatagcgtg catgtgttgc gttttcagag
     1801 tggcagtatc cagagtaata aagctgtaaa cgttacagga cgggtaacac cgtcagacta
     1861 cggaaacttt gatgcccgtt accagcagcg aaatggtggc gtgcaggatg tgcggtatgg
     1921 ttatgaaatg tattacaccc ccggcagtaa caccgtttcg tggacatttc gctcaccttc
     1981 gggacacggg ctgtcaggga tatcgatatc ggataccggc cgtaactcag cggataacgt
     2041 caacggcgtg tattaccgac cgctgcaaaa actgattaat ggcacctggt ataacgtagc
     2101 gagtatttaa caatgttgca tttaaaaaat attacggcag gtaatccgaa aaccgcggaa
     2161 taatatcagc tgacaaaaca gtatgatgtc acctggcttt tttcggaaga cggcaaaaac
     2221 tggtatgaag aacagaagaa ctttgccagt gacaccataa aaatggttta caccggagac
     2281 gggcgcgtgg tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt
     2341 attgaagttc ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac
     2401 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
     2461 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgctgct ggggctaatc
     2521 agcgacgaag accgggaaaa actgaaagcg taccgtattt acgcgaaatc gctgcaggcg
     2581 atggatttca gcaccatcac tgataaatcc tcatataacg ccattgaatg gtccgtctct
     2641 ccggaaggtt cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag
     2701 gtgcgctgac tgatggcgca ggcatcccca tgtccggatg ccagattatt ctgaaatccc
     2761 gtgtaaacac ctcagaagtg gtgatgcgta ccagggctga tgtagtgacc ggaaataacg
     2821 gtgaatattc gtttgaggca caggtcggaa aatattgcgt gtatctgaaa cgggactggc
     2881 gcgacgagta ctgtgttggc gacattgctg tatacga
//
LOCUS       DAZPLI010000284         2900 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZPLI010000284 DAZPLI010000000
VERSION     DAZPLI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.287"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..1199)
                     /locus_tag="ACHUU3_004298"
     CDS             complement(<1..1199)
                     /locus_tag="ACHUU3_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFR2084185.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
     gene            1492..1586
                     /locus_tag="ACHUU3_004299"
     tRNA            1492..1586
                     /locus_tag="ACHUU3_004299"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1526..1528,aa:Sec,seq:tca)
     gene            1858..2900
                     /locus_tag="ACHUU3_004300"
                     /pseudo
     CDS             1858..2900
                     /locus_tag="ACHUU3_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          845 a          691 c          665 g          699 t
ORIGIN      
        1 gcgctgttct gcgctttttc tgccgcatca tatccgccat aagccagcat ccagccgata
       61 agagcgccgc cgaaggccaa ccccagtttg agcacaaaca gcgtgccagc aaaactgatc
      121 ccggtcaggc gtttaccatt gcaccactcg ccgtagtcga cggtatcgga catcattacc
      181 cactggatag gtgtcaccag ttgatgcaac acaccaatca cgaagatgaa gacaaacata
      241 gtgatgctgg cctgcatggg aacaaagaac atcgcgaggc taatcactgc cagcagggcg
      301 ttcgtccacc agaagatagt gactttacat ttccagtcgg tcagaggttt tgccagtgcg
      361 gaaccaatca ggttacccac gcaataagtg gtgagaaaag cgacaaacac ttccggcgtg
      421 cccaaaatcc atgtgacgta atacatcatc gccccaccgc gtacgcacac cgccaggata
      481 ttgaaaatgg ttagtaaacc gacaatccgc cactggtcgt tttgccagat atcacgtaaa
      541 tcttcccgca tagacgttgt tgtaggtggt gcttcaacgc gttctttagt ggtgaagaaa
      601 caaaatgcca gcatcatgaa tgccaccacg gaaaggaccg cgataccgcc ctggaaaccg
      661 agtggtttat tatcaccgcc aattaaatta accagtggca tcatcagaac agtagaaagc
      721 atgcctcccg cggtcgccag cacaaaacgc caggattgca gcgagatacg ctgagtcggg
      781 tcattggtga ttacaccacc caatgcgcag taagggatat tgacgacggt ataaagtaag
      841 gtaagtaggg tgtaagtaat tgctgcatag atcattttgc cgttcatact gagatctggc
      901 gtgctatagg ccagtacaca gacgatcccg aatggcagtg cgccaaacag tacccacgga
      961 cgaaatttac cccagcgaga gcgcgttcga tcggccaaca accccatgca aggatcggaa
     1021 atcgcatcca gtgcacgagc gaccaaaaac atggttccga caaatccggc aggaatgcca
     1081 aaaatatcgg tataaaagaa catcatatat aacattacgt tatcgaaaat aatgtggctg
     1141 gcggcgtctc ccatgccata accaattttc tctttaacgg acaacacttc actcttcatc
     1201 tgcttttcct tgatgctata ggggtgctga gaccgataac gcattttgta aaccatccct
     1261 gtgagagcga atattccgat atctggttat taatttatga ttcttgtttt atgtgatcgt
     1321 ggtagcgtta attccgctca tatatcattg taaaatatgg gttttatatg agttataatg
     1381 ctttcgtgat aatacgctgc gtgtattagg cggaaaaaac tgatctgggg gatgtagaaa
     1441 ctcaaggaag tagctataat gcgccccgcc tccatgtagc aatcgaggcg cggaagatcg
     1501 tcgtctccgg tgaggcggct ggacttcaaa tccagttggg gccgccagcg gtcccgggca
     1561 ggttcgactc ctgtgatctt ccgccagttt acctcttctg aactctccca atgtcaataa
     1621 atctcttcta atcccttgta tatcagtatt tatttgtatc ctgaggtcaa cctacttcac
     1681 cccatatcaa gtttcttttg ggggtacttt agggggtgtt atttcctatt gaaatacaag
     1741 cacccccata ataggaaaaa tgtgatacat aacaacggct tttaacaata tctttgttat
     1801 acaaggggtg tattgggggt atcaggttag ttactggcag aaatccccct caggaacatg
     1861 aattcatcaa aagccggagg atgtcatggc attaaccgat atcaaagtga aaaccgcaaa
     1921 acccaaagac aaaccctata agcttgctga tggcggcggt atgtatctac tgattaacac
     1981 taatggttct aaatactggc gcatgaagta ccgctttgct ggtaaagaaa agatgctttc
     2041 tattggcgta taccccgatg taacgctagc cgatgctcgc gagaaacgca gtgaagccag
     2101 gaaactcctc gctgctggag gcgatccagg ggaggcaaaa aaggaagaaa aaatcgcgca
     2161 gcaaatgagt ctgaaaaata cgttcgaggc tattgctcgc gaatggcacc agtcaaaagc
     2221 tgatcggtgg tcattacgct atcgtgatga aatcattgat acctttgaaa aagacatttt
     2281 tccctacatt ggcaagcgcc ctattgccga aatcaaacca atggagttac tggaagcact
     2341 gcgtaaaatg gaaaaacgtg gagctctgga gaaaatgcgc aaggtccgcc agcgctgtgg
     2401 tgaggtgttc cgctatgcaa ttgttactgg ccgggcagac tataacccag cccccgatct
     2461 tgccagtgct ttagctacac cgaaaaaagt acattttccc tttcttactg ctaatgaact
     2521 tccccacttc ctcaacgatc tggcgggtta taccggaagt ataatcacta agacagctac
     2581 tcagatcatt atgttaaccg gcgtacgaac acaagaactg cgttttgcac gctgggaaga
     2641 tatcgatttt gagacaaaat tgtgggagat cccggcagaa gtaatgaaga tgaagcgacc
     2701 tcatattgtg ccgctctctg agcaggtcat tatgctattt aagcagcttg aaccaatctc
     2761 aaaacatcat cccctagtct ttatcggtag aaatgatccc cgcaaaccaa ttagtaagga
     2821 aagcattaac caagtaattg aattacttgg atataaggga agactcacag ggcacggttt
     2881 cagacacacc atgagtacaa
//
LOCUS       DAZPLI010000285         2877 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZPLI010000285 DAZPLI010000000
VERSION     DAZPLI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.288"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..953)
                     /gene="curA"
                     /locus_tag="ACHUU3_004301"
     CDS             complement(<1..953)
                     /gene="curA"
                     /locus_tag="ACHUU3_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR2084186.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDG"
     gene            1134..1652
                     /gene="mddA"
                     /locus_tag="ACHUU3_004302"
     CDS             1134..1652
                     /gene="mddA"
                     /locus_tag="ACHUU3_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR2084187.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1649..2098
                     /locus_tag="ACHUU3_004303"
     CDS             1649..2098
                     /locus_tag="ACHUU3_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR2084188.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2099..2332)
                     /gene="ydcY"
                     /locus_tag="ACHUU3_004304"
     CDS             complement(2099..2332)
                     /gene="ydcY"
                     /locus_tag="ACHUU3_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR2084189.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2418..2591)
                     /gene="ortT"
                     /locus_tag="ACHUU3_004305"
     CDS             complement(2418..2591)
                     /gene="ortT"
                     /locus_tag="ACHUU3_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR2084190.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2786..>2877
                     /gene="yncL"
                     /locus_tag="ACHUU3_004306"
     CDS             2786..>2877
                     /gene="yncL"
                     /locus_tag="ACHUU3_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR2084191.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWF"
BASE COUNT          691 a          738 c          692 g          756 t
ORIGIN      
        1 aaaccatcag taatatcttc gtggtagtgg attttatcct ctttcaccca ttgccccatc
       61 tccttctgaa actcatggat gcggtgacca taatcctgag cgataataaa cccttgcaag
      121 cgaatacgtt ttttcagcac cgttgccatc aacaggggta aacgatccgg accgggtggt
      181 agctctgtag cgttatagct gctcactaag ccacaaacgg gaatgcgcgc agatgtatta
      241 agtaatggta gcactgcatc aaatacctta ccgcccacgt tttcataata gatatcaata
      301 ccttttgggc acgctttcac cagttgttcg gcaaaatcat ccgcgtggtg atcaagacaa
      361 acatcgaagc ccagcacctc gatagcatgg cggcattttt ccgcgccacc ggcaaccccc
      421 accaccctgc aacctttaag tttgccgatt tgccccaccg ttgctcccac aggacctgtc
      481 gccgcagcta ccaccaacgt ttcgccctct ttaggctgac cgatatccag cagccccata
      541 taagcggtaa agcctggcat ccccagcaca cccagcgacc acgatggatt ttgcggatga
      601 tcgccaagtt tcaccagatc atcaccactg gatatgtcat agtcctgcca tccactgtag
      661 cccagcaccc agtcgccagg ctgataatca ggatgattcg actccacaac acggctcacc
      721 gtgccgccga ccatcacgcc gccaatatca acaggcggtg aataagatgg ctcatcgctc
      781 atacgtccac gcatatacgg gtccagggac aaataaactg tgcgcagtaa cacctgtcct
      841 tcacccggtg tggcgacatc atcttcttca agacggaaat tctccggaac cggtgcgcca
      901 tgtggacgcg aggccagaac ccaacgtcga ttacgctgct tttgttgccc cataatgctc
      961 tcctgtgctt ttggcatgaa tccatcaata gtagcaggct cacgggattg gttttgtttg
     1021 cagccgtcac atcccctctg caacacgcaa taatttcatc gaggaatccg ttgcttcggg
     1081 ctgttttcga tagtattgat aacatcatca tcattatttt atcgaggtca gacatgtcca
     1141 tccgttttgc ccgcaaagcc gattgtgctg ccattgcgga aatttataac cacgccgtgt
     1201 tgtatacggc ggctatctgg aatgaccaaa cggttgatgc agataaccgc attgcctggt
     1261 ttgaagcgcg gaccatagca ggttatccgg tgctggtaag tgaagaagat ggcgtagtga
     1321 cgggatatgc ctcgtttggc gactggcgta gtttcgatgg ttttcgccat accgtggaac
     1381 attcggttta tgtccatccc gatcatcagg gcaaaggtct ggggcgtaaa ttgttaagcc
     1441 gattgattga tgaagcgcgg gattgcggga agcatgtcat ggtcgccggg atcgaatcgc
     1501 aaaatcaggc ctcgctgcat ctgcaccatt cgctgggatt tgtcgtcacc gcacaaatgc
     1561 cgcaggtggg cactaaattt gggcgctggc tggatctgac atttatgcag ttgcaactcg
     1621 acgaacgcac tgaaccggac gcgattggat gaatcagtcg ctcacccttg cttttttgat
     1681 tgccgccgga attggtctgg tagtccagaa cacgctgatg gtgcgcatca cccagacctc
     1741 ctctaccatt ctcatcgcca tgttgctgaa ctcactggtg gggattgtgc tctttgtttc
     1801 tattttatgg tttaaacaag gcatggcggg gtttggcgaa ctggtgtcca gcgtgcgctg
     1861 gtggacactt atacccggct tgctgggatc gttttttgtc ttcgccagta tcagcggata
     1921 tcagaatgtg ggagcggcga caactattgc agtactggtc gccagccagc taattggcgg
     1981 tctgatgctg gatatcttcc gcagtcatgg cgtaccacta cgggcgttgt tcggcccaat
     2041 ttgtggcgca attttacttg tggttggcgc ctggttggta gccagacgct cattttaatc
     2101 agaggatggt gccgcctttg gttaactgtt cgtggcgcgc ttccatatct tctttatgct
     2161 tgcgaccgtg atgagcaata gcggtgcgca aacgctgctg ctgggtgtag cgatcttccc
     2221 gacttaactc cgcgtcatcg ctcagggcga ttaataattc gttcatatgg gcaatgacgc
     2281 tctcttcaat cgcggcatcc acgcgcgcga tgacttcatc cagatgcgac atactttctc
     2341 cttaaccact tgtgaaattt atcgattcat ccaaatgata taagtctgcg tggtgccgtc
     2401 aagccagtga gtaagattta aaacagtgaa aacaacagcg ccaccgggaa actcatcggc
     2461 catgttgccc ccaccagaaa ggcgcttaag aaacggatgc gtttcttatc gtgagaaaga
     2521 aaccaggtga taagaaatgc gattgctgcc ataaccgcat aaaaaacaag catatgttga
     2581 tagagagaca tgtacgggcg tccagccaga tgaataaaac gcgcgcaaat atgcgtcatt
     2641 ttttgacgtg catcaagttt tttcaccagt ttgagaaaaa atcatcaaac agcaacttgc
     2701 ccttatccat cttcagaatg agtattattg cggattttct taacccgtac tatacacata
     2761 ggggcaatga taaaaggtgg caaaaatgaa tgtttccagt agaactgtag tactgataaa
     2821 tgtctttgct gctgttggtt tgtttactct tatctctatg agatttggct ggtttat
//
LOCUS       DAZPLI010000286         2847 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZPLI010000286 DAZPLI010000000
VERSION     DAZPLI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.289"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(119..1102)
                     /locus_tag="ACHUU3_004307"
     CDS             complement(119..1102)
                     /locus_tag="ACHUU3_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR2084192.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFE
                     LLNQLVESNEKELNDCNLSGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            complement(1187..2212)
                     /locus_tag="ACHUU3_004308"
     CDS             complement(1187..2212)
                     /locus_tag="ACHUU3_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HFR2084193.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            complement(2238..>2847)
                     /gene="rfaY"
                     /locus_tag="ACHUU3_004309"
     CDS             complement(2238..>2847)
                     /gene="rfaY"
                     /locus_tag="ACHUU3_004309"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFR2084194.1"
                     /translation="LAYNFKTVKVLRNINDTKVSLIDTKYGRYVFKVFAPKTKRNERF
                     LKSFVKGDYYQNLIVETDRVRSAGLTFPNDFYFLAERKIFNYASVFIMLIEYVEGVEL
                     NDMPIIPEDVKAEIKASMEKLHALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAERK
                     ARDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA"
BASE COUNT          907 a          556 c          435 g          949 t
ORIGIN      
        1 tcaaagaaaa aatacataat atgagaatta aagactttat accagaatta atttcattcc
       61 aacaaaatga catatagttt caacctatgc tacgaggacg gattatttaa tgcaagatct
      121 aacgtttatt ctttttccta tttaccagtc ttaccatcct ccagagaata taattttttg
      181 atgctaatat ttttgctcgt cttttatctc tcttgccaga aagattacaa tcattcaatt
      241 ctttttcatt gctttcaact aattgattta atagttcaaa atcaccatta agtagaacat
      301 tccataaata acgagtttta tgataagctc tattttgact caataatgta tgaatttctt
      361 ctttcctgtt acctttgtat aactgcatca cagaagagta gattctttcg tgcgcaaatg
      421 actcatgtcg ataatgagtg atggttctac atgagttcat taaggaattt tctctatagt
      481 tatgcttata catataatat ggaaccgcat acatatcgca tgtaattagc agtcgataga
      541 taaaatccag atcttctcct agtcgtaaat cttcatcaaa aaaaagatta ttctcttcca
      601 gaagttgttt tcttataaac aagcacccta tatggaatct aaccttttta taaaaaaagt
      661 cgaaaagtaa agatccttca ttaaactctg tatttatagg tgagtaagta acaccatctt
      721 ttatataatg gccataaagc ccacaataaa tcgcatcccc tcccgtctca tgttgtctgg
      781 tatacatctt cagcagatag ttaggatcta tctcatcatc atcatccata aaacatataa
      841 actgtccttg cgccttgcgg atacctgtat tcctggcaga cgaaacaccg ccattttctt
      901 tattgataat aattaatcga gagtgggtta acccatactc tctcaaaacg tttgatgtgt
      961 tatctttaga accatcatta acaataacaa tttcaaaatt tgggtaagtt tgtttttcta
     1021 atttcgttag agtttcagta atgtaatcag atgaattata cgtcggtatt attatggata
     1081 ctaaagggta atcattgctc atcgtgttct cttgcctgaa ctaataaaat atgatagtac
     1141 tcatccttaa ttattattgt aactcagaca tccatgatct ttattattat ccaaatagtt
     1201 ttcttttata cttatttaat ttaaatttaa taaagtaaat aaaagcgtta aacgtatcac
     1261 catttttctt tgcatgttga tatttcactt gatactgctt ttcattcgtg gctggaataa
     1321 acgccacatc attccagcaa gaagcctgat aagcctcatt aaaatacttc tggcagggat
     1381 aattcgtcca gtcgttccag ggcttcgtta cgccaacata atgaatcagc ttagtatcat
     1441 cggtaatggt ctttttataa tcttcatcgc ttttattttt taactcataa tcgatgccat
     1501 aaaagcaatc ataatcgccg ctaataaaaa tacaatgacc aaagaacaga atatttaaaa
     1561 tatcctgatc aaggcattta tacttcgtgt gctgcgggtc acttgccagc atagcaatgg
     1621 ctttattcat aaaatcattt ttcgcccatg cttcaagctg cagatatact acaccggcgt
     1681 tgaaataatt ccctggaagt ccctctatag ccagacgctt cgctggtttt tcctgcataa
     1741 aaggcacatc tttaataaca gctgcaaact tatccccatc aaaattaata tcaagcaatc
     1801 cagctaatga gcctttacag atgacatcag catcgatata taacagattt tcaatagttt
     1861 cactcagata ctggaacgca actaaacgga agaatgttgc ataagaccac tgtttcaggc
     1921 tggggaagat atcaataaag gcactggaga ctttataaat tcttacttca acgttagcat
     1981 ttttgctggt aaaagcatta acacgttgta gataatcttc atcaatgtag tcggtaaaga
     2041 gatgaaactt aaccgcaagg tccggattat gcataacaac tgattgcata gatactgccg
     2101 caccaaataa aaagttacgg tcaataccgt aagcgatatt taacacacga tcggtgttag
     2161 caatttcccc agagacagcg acttcagtaa tactctcagc taataaatcc atgttggttc
     2221 cgttttgact gtgtggttta cgctttgcct tttaattttt taataaattt gcgtagtttg
     2281 gttctgtaga ttactgaata gtagccataa tcttttattt cgttggcaat acccaggtga
     2341 cgttccatgg ccagacgatc acgagcttta cgctctgccg tacagctttt accggatagg
     2401 tcaattatcc tgacaccatc tttactaacg ataaagttac cccggtgtgg gtcgcccgat
     2461 agcatattta aggcatgcaa tttttccatt gaagccttaa tttctgcttt aacatcttct
     2521 ggaatgatcg gcatatcatt gagctctacg ccctcgacat actcgataag catgataaaa
     2581 acactggcat agttgaaaat tttacgctca gccagaaaat aaaaatcatt aggaaaagta
     2641 agacctgcac ttctgacacg atcggtttca acgatcaaat tctgatagta gtcacctttt
     2701 acaaaagatt ttaaaaaacg ttcattcctt ttcgttttcg gggcaaagac tttaaaaaca
     2761 tagcgaccat atttcgtatc aatcaatgat actttggtat cattaatatt acgtaatact
     2821 tttactgttt taaaattata ggccagt
//
LOCUS       DAZPLI010000287         2797 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZPLI010000287 DAZPLI010000000
VERSION     DAZPLI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.290"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..972
                     /locus_tag="ACHUU3_004310"
     CDS             <1..972
                     /locus_tag="ACHUU3_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR2084195.1"
                     /translation="SLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSASLTFSRINWE
                     EDNQDQLYLNISIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDND
                     EFKDMKASLRASYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSG
                     SADSRFMIDADGAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIE
                     NSVITTTLTEGAIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVAD
                     GGFVYLNGIQDDSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            988..1737
                     /locus_tag="ACHUU3_004311"
     CDS             988..1737
                     /locus_tag="ACHUU3_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR2084196.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            1739..>2797
                     /locus_tag="ACHUU3_004312"
     CDS             1739..>2797
                     /locus_tag="ACHUU3_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084197.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT          877 a          564 c          583 g          773 t
ORIGIN      
        1 agtttggctc gtaatactta ctgggacgcc agcagtaatg tgaattattc attatcactt
       61 agccgcgatt ttgatatcgg gccattaaaa aacgtctccg cttctctaac atttagccga
      121 ataaactggg aagaagacaa ccaggatcaa ctgtacctaa atatttcgat tccctgggga
      181 accagtagaa cattgagcta tggtatgcaa cgaaatcagg ataacaagat ttcgcatact
      241 gcttcgtggt atgactcttc cgatcgaaat aattcctgga gcgtttctgc ttcaggcgac
      301 aatgatgaat tcaaagatat gaaagcgtca ctacgcgcca gttatcagca taataccgag
      361 aacggtcgtc tctatctctc cgggacatca cagcgagaca gttattattc tctgaatgcc
      421 agttggaatg gttcattcac ggcgactcgt cacggagccg ctttccatga ctatagtggt
      481 agtgctgact cgcgttttat gatcgacgca gatggtgcag aagatattcc gttgaacaat
      541 aaacgcgcgg taactaatcg gtatggcatc ggagttattc catcagtcag cagttacata
      601 acaacatcat taagcgttga tacccgaaat cttccagaaa atgtggatat cgaaaactcg
      661 gttatcacca ccaccttaac cgaaggtgct attggctacg ccaaacttga tacccgcaag
      721 ggctatcaaa tcatgggggt tattcgcctg gcagatggta gtcacccacc actggggatt
      781 agcgtaaaag ataaaaccag ccataaagaa ttaggactgg ttgctgatgg cggctttgta
      841 tacctcaacg gcattcagga tgacagcaaa cttactttac gctggggtga taaatcctgt
      901 tttattcaac cacccaatag cagcaactta accaccggaa cggttatttt accgtgtatt
      961 agccaaaatt aattcaggat gaaactaatg cgctacttaa acacaaagaa tctcattgca
     1021 gcaggagtat tactcgcctg catgagcagc attgcctggg gtgccatcat cccggaccgt
     1081 acccgtatca taatgaatga gtcagataaa ggggaagcat taaaacttac taatcaaagc
     1141 aaaaatctac cctatctggc gcaaacatgg atagaagata cgaaaggcaa taaatcacgt
     1201 gactttattg tcacggttcc ccctatggta cgtttaaatc ccagtgagca aattcaaata
     1261 agaatgataa gtcaggaaaa aattgctcaa ctgcctaatg acagagaaac gctcttctat
     1321 tttaacgtgc gagaaatacc gccaaaaaca gataaaaaaa atgtgatgca agtcactatg
     1381 cagcacgcat tgaaactatt ctggcgacca aaagctattg aattagaaga tgatggggta
     1441 atgtcttacg aaaaagttga aatcatccgt agaaatgatg gttcgatccg ctttaacaat
     1501 aagatgcctt atcacgttac gctgggttat atcggaacaa acggaatcac aatgttgcca
     1561 caaacgcaaa gtttaatggt aaccccttat agccatgcag atacgcagtt taaaaatgtt
     1621 ccgtcagctt ttcaggttgg ttatatcaat gattttggtg gattaagttt ttatgaaata
     1681 aactgcccga cggttaataa ctcttgtaat gtttccgtag ccaaacgaga taaataatat
     1741 gcgctactta ttaattgtaa ttatatttat tataggtttt agtgcattac ctgtatgggc
     1801 aatggactgc tatgctgaac atgaaggggg aaataccgta gtcattggtt acgtaccaag
     1861 aatttctatc cccagtaatg gtaaaaaagg tgataagatc tggcaaagca gtgagtattt
     1921 tatgaatgtc ttctgtaata atgcattacc tgccccatca cctggagaag aatacccatc
     1981 agcctggact aatataatga tgtttttacc aggcggtcag gacttttata atcagaactc
     2041 ctatattttt ggcgtaacat acaatggagt tgattatgat tcgactgccc ctaatgctat
     2101 cgcagcacca gaatgcattg atataaaagg agcgggtacc tttaataatc actataaaaa
     2161 ccccgctgtc tgtagtggag gtcctgagcc tcaattatca gtaacctttc cagctcgcgt
     2221 acaactctat attaaattag caaaaaatgc caatagagtt aataaaaacc tggtgttacc
     2281 tgatgaatat atcgctctag agtttaaagg tatgagtggc gcaggagcta tagaagtaga
     2341 caaaaatttg accttcagga ttcgtggatt aaataatatt catgtccttg actgctttgt
     2401 taatgtcgca ctggaaccag ctgatggcgt tgttgacttt ggtaaaataa attcccgaac
     2461 aattaaaaat accagcgtta gtgagacgtt tagtgtagtc atgacaaaag atcctggtgc
     2521 ggcctgtact gagcaattca atattttagg gagctttttc actacggata ttttgagtga
     2581 ttatagccat ctggatatgg gtaatggact gctattgaag atatttcata aagatggaac
     2641 agcaacggaa tttaaccgct tctcacaatt tgcttctttt tcatcgtcta gtgcaccttc
     2701 ggtcaccgca ccattcaggg cagaacttag tgcgaacccg gcagagactg ttgttgaggg
     2761 accgtttagt aaagacgtaa tcctcaaaat cacctat
//
LOCUS       DAZPLI010000288         2703 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZPLI010000288 DAZPLI010000000
VERSION     DAZPLI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.291"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..247
                     /locus_tag="ACHUU3_004313"
     CDS             <1..247
                     /locus_tag="ACHUU3_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HFR2084198.1"
                     /translation="AALPVSWIQMLAGLALLSTIGGSLYQALHNERERERDAAVVAFL
                     VTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNRY"
     gene            complement(287..1495)
                     /locus_tag="ACHUU3_004314"
     CDS             complement(287..1495)
                     /locus_tag="ACHUU3_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR2084199.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMHRQLEYKQLWRGGQVLAVPPAYTSQRC
                     AYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            1503..2004
                     /gene="tnpA"
                     /locus_tag="ACHUU3_004315"
                     /pseudo
     CDS             1503..2004
                     /gene="tnpA"
                     /locus_tag="ACHUU3_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(2022..2690)
                     /locus_tag="ACHUU3_004316"
                     /pseudo
     CDS             complement(2022..2690)
                     /locus_tag="ACHUU3_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          625 a          665 c          666 g          747 t
ORIGIN      
        1 ggctgccctg cccgtaagtt ggatccagat gctggcaggt ctggcgctgt tgagtaccat
       61 tggcggcagt ttgtatcagg cgctgcataa tgagcgtgag cgtgagcgcg acgcggcggt
      121 ggtggcattt ctggtaacgg caagtggatt gacgctggtc gggattggtt ctgcgttctg
      181 gggattaatt gccggaggcg tttgttacgt ggtgttgaat ttaatcgctg acagaaaccg
      241 atattgacat cctccacgcc ctgaaggacg tggatccctg ctacgttcag gctgtcgcct
      301 gaatcatttc ggtgggttcc tgcttcaacg ggcggcctga ctgcaccatc tctccacagg
      361 caagaacggc gtgccccgcc gctaaaatgt tacgagcacc atttacatcg gcgttcgctg
      421 tatatccaca tacctggcat ctgaatttac tttgtgacag gcggttctct ttcgctgtat
      481 gaccacagta cgcgcaacgc tggcttgtat acgctggcgg aacagcaagc acctgaccgc
      541 cacgccagag ctgcttgtac tcaagctggc ggtgcatttc ataccagccc tgatccagta
      601 tcgaacggtt taaacctgat tttgcccgga cattgcgccc cggctggctg accgtacccg
      661 ctgctgactt tgacatgttg ctgaccttca aatcctcaat gactatcatt gcgtggtttt
      721 tgctgacggc cgttgtgact ttgtgaaggt agtccctgcg gatatttgcg atacaggaat
      781 gcagtcgctg tattttgcgt ttctgcttct gccagttgtt gctgaatttg accttgcggc
      841 ttaactgtcg ctgaagtctc gccagcgtct tctggttttt ctgaaaactg tttacaggct
      901 caaagactgt gccatctgac agcgtggcga gtttagccac gccagcatcc agcccgacca
      961 ttgatgctga agggtgagcc ggagtggata cttcactttc tgtctgaata ctgatgtacc
     1021 acgtaccgca ggactggctg acagtgacat ttttcacaac acccgtgacc tgacggctat
     1081 tccggtagcg catccagccc agtttcggca gaaaaatacg gctgttttcc tgatcgagct
     1141 taacgccctg cgggtagcgg aatgcatcat tctgtccccg ctttttgaat cggggaaaag
     1201 cagcccgctt ccggaagaag tttttgtaag cccgctcaag gtctttcagt gactgttgca
     1261 atggctgtga gggagaatct ttaagccatt gcgtttcagt ggcatttttc cactcaacca
     1321 gccaggaagc cattttcccg taagggatgt atttattacc ggcctcatga ttctcattct
     1381 gacgtgccag agcacgattg aaaacgaaac gacatgcgcc tgcgaagcgc ctcatttcac
     1441 actcctgttg accacctggt cttaactgga atttaaatgc ctgtaatcgt ttcacaggtc
     1501 aaatgatact ttggtctgtg aaaaaagaaa ccgatatccg gcgtggaagg cattgtgttt
     1561 tcctgatgca tgttcacctg gtctttgtca ctagataccg acgccagatt ttttgacgcg
     1621 acagaaaaac tacgcactta cttttcaaat gtatgtgctt attttgaagc tgaactggtt
     1681 gaaatggatg gcgaaccaga tcacgtccat ttgttaatta actaacctcc caaactggcg
     1741 atatccagtc tggtaaacag cctcaaaggc gtatcgggta ggttactgcg acgagatcga
     1801 ccagatattg cagtcaggta ttactacaaa ggcgttttgt ggagtcctgg ctattttgcc
     1861 agtagctgcg gaggtgtgcc aatatccgtc atccgccaat acattgaaca acagcaaaca
     1921 cctggtcagg tggaaaaccg cgccttatat ccccgccctg aaggatgggg ttttacggcg
     1981 caccggataa aaagcgccat ctaacgatgg cgcgtctgga actacttttt gttaacgtca
     2041 aacatggtgg catcggtcgc catgtcatca ataacttgtt taatattttc gaaagccatt
     2101 ggcgtacttt cgttattcag gtctttacct tcgccctgac gaacgacttt gataaccggt
     2161 ttattagtcg ctgcatcaat cagctcacct tcaaaataga ggcgagtatc catggtacgg
     2221 tggcctgtag ccatttgcgt ccccgcaacc actaatgcaa caggaaccac ttcatagaat
     2281 tgcagccctt ctttgctggt atctacaccg gtaatggccc cacggaaaat cagactacgc
     2341 ggcccagcgg tggtaaccag tggtttacgc tgcgctatcg cttctttcat ttcggtgttg
     2401 gtatagttca aaattttatc cagaactttc tgccctacct aggtagaagg ttttggcacc
     2461 ggataataag tgattgggtt ccagacgatg ctgtcatatt tgctttgatc aaaactcggg
     2521 tctacccaac gtaaaacagg tttacctgtt gctgaggttg tttcttttaa atcagagtaa
     2581 ttgtttaaaa aaccagaata tttatctggc tgggtgattt tagaggcaca accagacaga
     2641 gccaacaagc cgcataaagc tgcaactttc gcaaatgatg tggtacgcat gaatagtttc
     2701 ctg
//
LOCUS       DAZPLI010000289         2651 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZPLI010000289 DAZPLI010000000
VERSION     DAZPLI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.292"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(25..315)
                     /locus_tag="ACHUU3_004317"
     CDS             complement(25..315)
                     /locus_tag="ACHUU3_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR2084200.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(305..742)
                     /gene="ratA"
                     /locus_tag="ACHUU3_004318"
     CDS             complement(305..742)
                     /gene="ratA"
                     /locus_tag="ACHUU3_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR2084201.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            913..1395
                     /gene="smpB"
                     /locus_tag="ACHUU3_004319"
     CDS             913..1395
                     /gene="smpB"
                     /locus_tag="ACHUU3_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR2084202.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1610..1972
                     /gene="ssrA"
                     /locus_tag="ACHUU3_004320"
     tmRNA           1610..1972
                     /gene="ssrA"
                     /locus_tag="ACHUU3_004320"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2171..>2651)
                     /locus_tag="ACHUU3_004321"
     CDS             complement(2171..>2651)
                     /locus_tag="ACHUU3_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR2084203.1"
                     /translation="AVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAR
                     EARRQEIEAWRTEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWGD
                     ADNQQVKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGTIRAFDVT"
BASE COUNT          698 a          718 c          600 g          635 t
ORIGIN      
        1 caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg
       61 aagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac
      121 actatcgctt agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat
      181 atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg tcgcgccctc
      241 ctgcagcgtc acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc
      301 aattttacct agcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag
      361 acgccagctc tttaaacacg cgaccaaagg cgagttcaat caacttattg gtaaactcaa
      421 agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac ttccatccac
      481 caatcaattt cttgaacggc ccatccacca gattcataag aatactttgg ttactggtca
      541 actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag
      601 tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag
      661 gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg
      721 cggtccggct aatctgaggc ataactattt ccatcaacaa aaatccaaca aataatatca
      781 tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag
      841 atagagcctt gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga
      901 ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt
      961 aacaagcgcg cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg
     1021 caaggctggg aagttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctacgtc
     1081 cttctgcgtg acggagaggc atttctgttt ggcgctaaca tcacgccaat ggccgtggcc
     1141 tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa
     1201 ctggactcat tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg
     1261 tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac
     1321 gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcacg tatcatgaaa
     1381 aacgcccacc gttaaacctg cactccaatt attgaccagt tcctcaccgc gcctccctct
     1441 ccggcggcgc gaatgaacat cttattggct atcacatccg acacaaatgt tgccatccca
     1501 ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga taacgccatt
     1561 gaggctggtc atggcgctca taaatctggt atacttacct ttacacattg gggctgattc
     1621 tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa
     1681 aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct
     1741 tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa
     1801 aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt
     1861 gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt
     1921 accgaggatg taggaatttc ggacgcgggt tcaactcccg ccagctccac cacttttgac
     1981 aggactgcaa ccggacagag actataaaaa cagtcactta cggacactga ccagacagta
     2041 agcggaccag aaaaagacaa aaatatacac gcaaaatgca cgtgcatttc aaaaaagcct
     2101 ctgattatga agtcagaggc ttttctagtt gtaaataagg gtaacaattt aacacgatgc
     2161 agcttaccaa ttacgtaaca tcaaacgctc taattgttcc caaatcctcc agaccactca
     2221 gctcctcttt catttcacgc tgacggcgat aaatctcatc gttgcgatcg acctgcgcct
     2281 gcaccattgc tgccgccagt tcttccagtt ccggcatcga cagtttcacc tgctgattat
     2341 cggcatcacc ccacgccata tgcgtttgtg ctgtgacaga ttttgccagc atgactaccg
     2401 gggacaggcg gcccagtgag tcggggccag cattccagat acgaccattc cattcaaacg
     2461 tgaacggctt cgcctcctgt tctgtgcgcc atgcttcaat ttcctgacgt ctggcctctc
     2521 tggccgcttc cagcatttct ggtgtcacag tgaatggggc tatctcaccc catttgccac
     2581 tttgcagttc ctgccagatt tgctgacccg tcggtgcgac atcatcagcg gtggctgtgt
     2641 aggggactgc c
//
LOCUS       DAZPLI010000290         2587 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZPLI010000290 DAZPLI010000000
VERSION     DAZPLI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.293"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            196..1149
                     /locus_tag="ACHUU3_004322"
     CDS             196..1149
                     /locus_tag="ACHUU3_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR2084204.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="ACHUU3_004323"
     CDS             complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="ACHUU3_004323"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR2084205.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2481..2576)
                     /locus_tag="ACHUU3_004324"
                     /pseudo
     CDS             complement(2481..2576)
                     /locus_tag="ACHUU3_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
BASE COUNT          714 a          636 c          578 g          659 t
ORIGIN      
        1 tatttttttc ataataatgc cctcaattta aaaacataac ttattgcgat atggttacat
       61 taagggcaaa gcatctctaa tgactttagc tcaacgatta ataacatttt ttcattaatt
      121 taagttggct aaatgcagtt aaaattttta acggccagcc acccaaaatc gtcgcttcca
      181 ttttccggag taataatgaa gcatgaatta tcaagtatga aggcatttgt catactggca
      241 gagtccagtt cgtttaacaa tgccgctaaa ttactcaata ttacgcaacc tgcattaacg
      301 cgaagaataa aaaagatgga ggaagattta catattcaac tatttgagcg tacaactcgc
      361 aaggttacgt taacaaaagc aggaaaaagg ttgctcccgg aggcgcggga attaataaag
      421 aaattcgatg aaacgctttt taatattcgt gatatgaatg cttatcatcg tggtatggtg
      481 acgttagcgt gtattccgac cgcagtattt tattttttac cgctggcaat cggtaaattt
      541 aatgagctgt atcccaatat taaagtgcgg attctggaac aaggcacgaa taattgcatg
      601 gaatcggtgc tgtgtaacga atcagacttt ggcataaaca tgaacaacgt cacaaattca
      661 tccattgatt ttaccccact ggtcaacgag ccgttcgtgc tggcctgtcg gcgtgaccat
      721 ccattggcca aaaagcagct tgtagaatgg caggagttag tgggttacaa aatgattggc
      781 gtacgctcct ccagcggcaa ccgactgttg atagaacaac agctggccga caagccctgg
      841 aagctggact ggttttacga agtgcgtcat ctttcgacgt cattaggact ggtagaggcg
      901 ggactgggga tttcggcgct tcctggtctg gcaatgcccc atgcgcccta ttcttcgatt
      961 attggtattc cactggtgga acccgttata cgtcggacat tggggattat tcgccgcaaa
     1021 gatgccgtgc tttctccggc agcagaacgc ttttttgcct tgctgattaa tttgtggact
     1081 gacgataaag acaatttgtg gaccaatatc gtcgaacgcc agagacatgc gttacaggag
     1141 ataggttaat acaccggcgc aggagagact gcgccgggta aatcagcggt tagtgtgcgt
     1201 taaccaccac ccacattggt ccctgcccga ccgcatagcg gcctttttca tgcagtagcc
     1261 cctgctcgcc aacaatttcg tataccgaga tgtggtgaga tttttgcccg gcggcaatca
     1321 gatacttgcc gctgtgatca acattgaagc cgcgcggctg ggtttccgtt ggctggaagc
     1381 cttctttact caacacgctg ccatcttccg aaacgctgaa aacggtaatc agactggcgg
     1441 tacggtcgca ggcgtataaa tgacgaccat ccggggtgat atgaatatca gccgcccaac
     1501 aggtgtcgga gaagttttcc ggcatcatat ccagcgtctg gacacattca atattgccgt
     1561 gtggatcttt cagttcccag acatccactg agctgtttaa ctcattgacg caatacgcat
     1621 attgttcgtt tggatggaat accatatgac gcgggccggc cccttcaacg gtagtcactt
     1681 ccgcagggtc ctgcgccacc agatgaccat catcgctgac cgtaaacagg caaatgcgat
     1741 cctgctttaa tgccggaacc cacagcgtac ggttgtccgg tgagatattg gcggaatggc
     1801 aaccgtccag cccctcgacc acatcgacga cgcccactgg caggccatct tccagacgcg
     1861 ttacgctcac gttacccgca ttgtaagaac ctacaaagac aaactgcccc tggtgatcgg
     1921 tggaaatatg cgtcggacta cccggcagcg cagactctgc ggcaaaggtc agtgcgccat
     1981 cgtccggggc aatacgatac gccaggacgc gaaactcagg gcgaacacca acatagagat
     2041 aacgtttgtc cgggctgacc accatcggct gcacctgccc cggcacatcg acaacctgtg
     2101 tcagcgtcag tgcgccttca tgattcaggt tccagacgtg aatttgctgg ctctcagggc
     2161 tggcgatata aactgtttgc ttcatgaaca cacctcaaaa atgataaatc ccttatcttt
     2221 catacacata acaacactta ctggataaaa aacagacaac aaaacacgat aaaatacaat
     2281 atcgcacatc aattaaatca ataagttagc actccatttc gaatggcata ttactaaaat
     2341 attagtcacc gacacgaatt tgacaatgcc acagatattt tcgcgactat tcagggagta
     2401 tgactctccc cgcttttgtt tacctgtatc aacgaccgta aacactatgc cataacaact
     2461 tctggccaac ttcccattcg ctcaacaatc aactcacttc cggcacaaat aaacgcatta
     2521 cgttgtagat cggtattctg gtcatttgtt gatacgcgta catagccaat aagcatggta
     2581 aatcccc
//
LOCUS       DAZPLI010000291         2582 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZPLI010000291 DAZPLI010000000
VERSION     DAZPLI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.294"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..402
                     /locus_tag="ACHUU3_004325"
     CDS             <1..402
                     /locus_tag="ACHUU3_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFR2084206.1"
                     /translation="IIKECIENGINSTPSTGYQPLILQSIKTKLLSSRLNKFNDFEDS
                     FNGLGISVHDISAQKISLLSFQKYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFF
                     RIWFFLQRHKDFAFKPFFTNFNTIERIENYL"
     gene            399..878
                     /locus_tag="ACHUU3_004326"
     CDS             399..878
                     /locus_tag="ACHUU3_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001688100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFR2084207.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(932..1882)
                     /gene="corA"
                     /locus_tag="ACHUU3_004327"
     CDS             complement(932..1882)
                     /gene="corA"
                     /locus_tag="ACHUU3_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR2084208.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(2034..2090)
                     /gene="ysgD"
                     /locus_tag="ACHUU3_004328"
     CDS             complement(2034..2090)
                     /gene="ysgD"
                     /locus_tag="ACHUU3_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR2084209.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            2252..>2582
                     /locus_tag="ACHUU3_004329"
     CDS             2252..>2582
                     /locus_tag="ACHUU3_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084210.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          755 a          590 c          561 g          676 t
ORIGIN      
        1 ataatcaaag aatgtatcga aaatggcatt aattcaactc cctcgacagg ataccaacct
       61 cttatcctgc aaagcattaa aaccaaatta ctttcatcaa gactaaataa atttaatgac
      121 ttcgaggaca gttttaatgg cttaggaatc agtgttcatg atatttcagc tcaaaaaata
      181 tctcttttaa gttttcaaaa atacgctata ggatggagtg caacaattca ttttgtagca
      241 caggaccact tcggtcttga tgtaactgat atcaaaaaca agacttacag caaatataga
      301 ttttttcgca tatggttctt tttacaacgg cataaagact ttgctttcaa accttttttc
      361 accaatttta acactatcga aagaatcgaa aattatctat gaaaagcaaa ctccagaaaa
      421 ttatattatg tttatttctt ctatgctgca tctataattt atggacattg cgtcctgtac
      481 agattttgta tacatactct gatgctggca attcagtctt tctggtcgtt gatcatctgc
      541 catggactga cagcgataaa ataaattggt atctgaagca tcagaatgaa attaaaaatc
      601 aacatcccct accagaaggt agttggcata cctggtatgt cattgatatc ggtaatggct
      661 tcactgacta taaaaaatat atcgaagggc cgtatgaaga tctctactgt ttcccgacaa
      721 ttaagagcaa tgataattgc atcgtgaaaa attacttaat ggttattaat gaataccctt
      781 atcgtaacac tcatattggt attaacgatt ttactgagta tcaattaaca caagaaaata
      841 aaatagagcg agttttcaat ccgcatgact ttaaataaga caatttttag aaataagccg
      901 gataaaaaaa tagctttatc cggcactcat attacagcca gttcttgcgc ttaaagtaca
      961 gatacggtgc caggcccgcg aggatcataa agataatcgc gcctgggtag ccgaaactcc
     1021 acttcagttc tggcataaac tcaaagttca tcccatagct ggaagcaacg agcgtcggcg
     1081 gcaggaatac cacggatacc accgagaaga ttttgatgat gcggttctgc tcgatgttga
     1141 taaaacccat cgccgcctgc atcaggaagt tcaccttctg gaacagggat tcgttatgcg
     1201 gcagcaggga ttcgatatct cgcaggattt cacgcgcctg ctccagttgc ccacccggta
     1261 aacgcgcttt acgcaccagg aagttgagcg cgcgctgggt atccatcaga cacaggcgaa
     1321 ctttccagcc gatatcttcc agttccgcca gagtggagag cgcctcgtcg tactcatcgc
     1381 cctgatgccc ttccataatc acccggctca actgctccag gtcgctatag atattttcaa
     1441 tttcatctgc caactgttcg attttggttt cgaacagatc cagcagcagc tcgtaggcgt
     1501 taccgtctac catcgactgg ctacgggcac gcatacgata cagacgaaaa gcgggcagtt
     1561 cacgctcacg cagagtaaac agacgaccat cacggatggt aaatgccaca gtggagttac
     1621 cggcgtgatc ttccgcatct tcaaagaaga agaaggagtg aatatgcagg ccgtcgtcgt
     1681 cttcaaagaa acgtgccgat gcttcgatgt cttccagttc cgggcgggtt gccaggctct
     1741 ggccaagttc agattgtacg cgcagtcgct cgtcgtcgtc cggttcgaca agatcaatcc
     1801 atactgcatt tacaaggggt tgtgactctt cgacttccag ccgggtcagt cggttatttt
     1861 ccagttgaaa tgcgctcagc atgaccggga ctcccaatgc ttaaaatatc ggacagttcg
     1921 gtgggcacac agaaacaaat tgggtttcag accattaaac agcctgactc agcgcgacgg
     1981 gaaaaattga ggtcgctgac aaccgctaag gctatcagca aaaagggata gccttaggag
     2041 ttgatcctgg atgacaggat agtgagccag tatctactgg gtgtgtccaa ggcgaatgtc
     2101 ctcttagagt gatcgtgcgc gcatgttacg ccagcaaaat tttgccgtca acacgcaaca
     2161 gaacaccaca gaacaatagt tgaacttcgc gctttttggt actcgtcaag gtcgctaaca
     2221 ggataaaaaa atccaacatc caggacctat aatggcgcat cggctactca ttggtaaagg
     2281 aatgatcacc ttgaatctca aacggatttt tctcgccctc accttacttc ccttgtttgc
     2341 tgttgccgct gatgattgcg cactctcaga tccgatgctg accgtacagg cgtataccgt
     2401 taatcctcaa acagagcggg tgaaaatgta ctggcaaaaa gccaatggcg aagcgtgggg
     2461 aactttacat gctctgctgg tggatatgaa tagtcagggt caggtgcaga tggcgatgaa
     2521 cggcggcatc tatgatgaaa gctatgcgcc gctcggtttg tacatcgaaa acggtcagca
     2581 ga
//
LOCUS       DAZPLI010000292         2561 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZPLI010000292 DAZPLI010000000
VERSION     DAZPLI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.295"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..81
                     /locus_tag="ACHUU3_004330"
     CDS             <1..81
                     /locus_tag="ACHUU3_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084211.1"
                     /translation="REWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(98..907)
                     /gene="proC"
                     /locus_tag="ACHUU3_004331"
     CDS             complement(98..907)
                     /gene="proC"
                     /locus_tag="ACHUU3_004331"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR2084212.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1027..1485
                     /locus_tag="ACHUU3_004332"
     CDS             1027..1485
                     /locus_tag="ACHUU3_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR2084213.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1668..2192
                     /gene="aroL"
                     /locus_tag="ACHUU3_004333"
     CDS             1668..2192
                     /gene="aroL"
                     /locus_tag="ACHUU3_004333"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR2084214.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2242..2433
                     /gene="yaiA"
                     /locus_tag="ACHUU3_004334"
     CDS             2242..2433
                     /gene="yaiA"
                     /locus_tag="ACHUU3_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR2084215.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
BASE COUNT          642 a          630 c          678 g          611 t
ORIGIN      
        1 cgtgagtggt tgaaatcggc agatttggcg ctttacaaag caaagaaagc cggacgtaac
       61 cgcaccgaag tggcggcctg acgtccggcg aaagtcatca ggatttgctg agtttttctg
      121 atttttccat acacttcgtc atcgcttcga tcactgcggc acggaagcct ttctcttcca
      181 gtacgcgtac cgcttcaatg gtggtgcctc ccggtgagca gaccatatct ttcagtgccc
      241 ccggatgttc tcccgtttcc agcaccattt ttgcggaacc cattaccgcc tgagcggcaa
      301 atttatacgc ctgggcgcgt ggcatcccgc ccagcacggc ggcgtcggcc atcgcttcga
      361 taaacataaa tacgtaggct ggcgaagaac cgctcacacc gaccaccggg tggatcatcg
      421 gctcagcaat tacttccgct tcgccaaagc agcggaaaat attcagcaca tcagcggtat
      481 cttcgggggt taccagcgcg tttggcgtta cggaggtcat cccggcatta accagcgcgg
      541 gagtgttcgg catggcgcgg ataattttcc ggtcatggcc cagcgcgcgg gcaagctggt
      601 cgagcgtgac acctgcagca atagaaacga ccagagagtc tttattcagg ctggaggtga
      661 tttcgctaag cactttaatc atgatgccag gtttaacggc ggcaaaaatg atgtcggcga
      721 tttgcgccac ttcttgcgcc gattctgcgg cgttgatgcc gaactggtca tgcagggcgg
      781 cgactttatc cggggagggg gtgtataccc agatttgccc tggaagcacc tgaccgctgg
      841 caatcagacc gccgagaatg gcttttccca tattgccgca gccaataaaa ccgattttct
      901 tttccattgc ctcactcctg ccgtgaaatt cattgttttg ataatcgctg gcagaagcat
      961 aaacagaact atgccggaag gcaaaagcgc gacacaatag aggattaccc aacaaaggat
     1021 gactttatga caatttgggt ggatgccgac gcgtgtccca atgtaattaa agagattttg
     1081 tatcgcgcgg cggaacgtat gcagatgccg ctggtactgg tagcaaacca gagtttacgc
     1141 gtgccgccat cgcgatttat tcgtacgctg cgcgtcgcgg caggtttcga cgttgccgat
     1201 aacgaaattg tccggcagtg tgaagcgggc gatttggtga tcaccgcaga tatacctttg
     1261 gctgctgaag ccatcgagaa aggcgcagcg gcgcttaatc cgcgcggcga acgttacacg
     1321 ccagcgacca ttcgtgagcg cctgacgatg cgcgatttta tggatacctt acgtgccagt
     1381 gggatccaga ccggcggacc agatagcctt tcacaacgtg accgccaggc ctttgccgcg
     1441 gagctggaga agtggtggct ggaagtgcaa cgtagtcgtg gctaaatgta atttattatt
     1501 tacacttcat tcttgaatat ttattggtat agtaagggat gtattgagat tttcacttta
     1561 agtggaattt tttctttaca atcgaaattg tactagtttg atggtatgat cgctattctc
     1621 atgacaccgg ctttcgccgc attgcgacct attggggaaa acccacgatg acacaacctc
     1681 tttttctgat cgggcctcgg ggctgtggta aaacaacggt cggaatggcc cttgccgatt
     1741 cgcttaaccg tcggtttgtc gataccgatc agtggttgca atcacagctc aatatgacgg
     1801 tcgcggagat cgtcgaaagg gaagagtggg cgggatttcg cgccagagaa acggcggcgc
     1861 tggaagcggt aactgcgcca tccaccgtta tcgctacagg cggcggcatt attctgacgg
     1921 aatttaatcg tcacttcatg caaaataacg ggatcgtggt ttatttgtgt gcgccagtat
     1981 cagtcctggt taaccgactg caagctgcac cggaagaaga tttacggcca accttaacgg
     2041 gaaaaccgct gagcgaagaa gttcaggaag tgctggaaga acgcgatgcg ctatatcgcg
     2101 aagttgcgca tattatcatc gacgcaacaa acgaacccag ccaggtgatt tctgaaattc
     2161 gtagcgccct ggcacagacg atcaattgtt gattttagag cgcctatact taacgttcat
     2221 cccgtgaaat aaggaagaac gatgccaacg aaaccgcctt atcctcgtga agcatatata
     2281 gtgacgattg aaaaaggaaa gccaggacag acggtaacct ggtaccaact cagagccgat
     2341 catcctaaac cagactcgtt gatcagtgaa catccgacgg ctcaggaagc gatggatgcg
     2401 aaaaaacgct atgaggaccc tgacaaagag tgaccgcatc agactgctcg gaagggattc
     2461 tgagtgccac tacaagggat ctgcgtcaca tttttcataa ttcatgtttt tctaataatt
     2521 agaatattaa acaataacaa tccattactg gaatcatttg g
//
LOCUS       DAZPLI010000293         2554 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZPLI010000293 DAZPLI010000000
VERSION     DAZPLI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.296"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHUU3_004335"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHUU3_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR2084216.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="ACHUU3_004336"
     CDS             complement(390..1178)
                     /locus_tag="ACHUU3_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR2084217.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSWIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="ACHUU3_004337"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="ACHUU3_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR2084218.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHUU3_004338"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHUU3_004338"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR2084219.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2092..>2554)
                     /gene="pagP"
                     /locus_tag="ACHUU3_004339"
     CDS             complement(2092..>2554)
                     /gene="pagP"
                     /locus_tag="ACHUU3_004339"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR2084220.1"
                     /translation="FRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPWG
                     GGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTAG
                     VTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          653 a          659 c          579 g          663 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cagctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgg attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
     1981 ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg
     2041 ttacttattt tagttattga ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg
     2101 aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat
     2161 ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat
     2221 gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt
     2281 ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt
     2341 gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca
     2401 tcgcgactga ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt
     2461 gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg
     2521 ctgttgccag gtttgtgcaa tattttctct aaac
//
LOCUS       DAZPLI010000294         2538 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZPLI010000294 DAZPLI010000000
VERSION     DAZPLI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.297"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..503
                     /locus_tag="ACHUU3_004340"
     CDS             <1..503
                     /locus_tag="ACHUU3_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR2084221.1"
                     /translation="HNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNN
                     KILKHIKSHLIMLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTID
                     GLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSY
                     REVNRD"
     gene            496..978
                     /gene="yqeJ"
                     /locus_tag="ACHUU3_004341"
     CDS             496..978
                     /gene="yqeJ"
                     /locus_tag="ACHUU3_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFR2084222.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1012..1092)
                     /locus_tag="ACHUU3_004342"
     CDS             complement(1012..1092)
                     /locus_tag="ACHUU3_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084223.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1128..1552)
                     /gene="yqeK"
                     /locus_tag="ACHUU3_004343"
                     /pseudo
     CDS             complement(1128..1552)
                     /gene="yqeK"
                     /locus_tag="ACHUU3_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
     gene            1982..2472
                     /locus_tag="ACHUU3_004344"
                     /pseudo
     CDS             1982..2472
                     /locus_tag="ACHUU3_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          948 a          418 c          410 g          762 t
ORIGIN      
        1 tccataatga cattaacata caaaatcatg taataaactc gtcaacagac gcacatacac
       61 acaatgcccc acctgccata aaagttaatg cgggatacaa agagagcatt ggtggcgcaa
      121 agaatttcaa taacaaaatc ctcaaacata taaaaagtca tctaattatg ttgagcgcat
      181 ttgtcatagg cgcatattct gcatattggc tatggaataa taatcaaccg aagccatttt
      241 tcaaggatta caagactgtt gcggaaatta atggctgcca ttttaatgta acagaagata
      301 caattgatgg gttgaaagag ttcgataaat ataaaacacg gatactggat tcggggatta
      361 actgtaaaaa atatccgtgg ttatacttcc ctcttgcaaa atcctcaccg gggatgattg
      421 ttatggcatg caataaaaac tataaccaac atgaagtggc agactgttta accctgtctt
      481 accgtgaggt taatcgtgat tgattataaa aaaaacttat tatttatttt agtttttatc
      541 agtgggttta ttctcttcac tgtctattct tacactgcag aaaaaatgat atataacgaa
      601 acatgcactg caaattgggt aatttttaat gaccagggac gagcaaatct taccatcgac
      661 tttatgtata acaaaaaaaa taaaacagga actgttgcgt tgagtggaac ctggcaacaa
      721 ggtaacagag aaagtaaatc aatacggcga aatattgaat acacatggat tgaaaactat
      781 gacacagccc atttaacatc aaagaaagtt aataagtttg agattatgga tcaagttgat
      841 gatgatagac ttgcacaact tattcctgat ttttatgtct ttccagaaaa aagtgtaagc
      901 tataatatac taaagcaagg taagcatgct tttattttga gcattggtaa cagagcaata
      961 atgcattgtg caaggtaaac taaaatagat aacaaaaaag atggaacctc gttaatcata
     1021 aaacaataaa attaaatatt ctcgcagtat atggcagtct aaagcatcaa agttttgatc
     1081 aacatctttc attttagaca tctccttgca atgtaaaata tcatgtatca acaacatctg
     1141 gtgcaaatcc atgagtcgaa ctcgacattc atcttagctc gattattatt ccttcgtaca
     1201 gtcgatgtaa acaatacaga aagaggatta ttaagaacca gtttactcag gaatcaatag
     1261 aggaaaggac taacgtttct ttaaaagaat tgatttcatc atctgttaaa ctaaactcat
     1321 cattgacaga tcgtgagata taactgtttt taactttact ctttacgttg actttattga
     1381 cagaattaac attcacatat cttgaattta atgtccatga tgttgtttgc gtgagaacat
     1441 tctcagcatt aaggaatttt tttaacggaa tccttggttt ctttttcgag tccgaattta
     1501 caatatcatg catatagacc atttcatgaa tctgcgaaaa ctcaatgtcc attgtatacc
     1561 tcacattttt accgtgactc gatgttactg ttcaataatc accttccatc aatactaaaa
     1621 ttaatacccc taatgtgccg ataacaaata tagtcattct acgtaacgtc tccataaggt
     1681 gataaccttg atcaaatgaa tttcgaatat aataaaggaa atcttttgag atgtttatca
     1741 cgaataaagc tatttatgtt accaatatca tttcgagtgc gcacactatc tatctgtttt
     1801 ttcgagatat aaaaaacaac gtgtaagtta tgtaaattgc cgatgctact gagtaacatt
     1861 atggtaaaaa aagtaaaaat gagcgatttt tgatagaata aagcatgaga aaaggtgata
     1921 tttgacatta tcagaagctg cgaattcgga ttttgctcta atcaaatgag agaaatgtaa
     1981 aatgaagcca cgaaatatta ataatagcct accactgcaa ccattagttc ctgatcagga
     2041 gaacaaaaat aagaaaaatg aagagaaatc cgttaatcca gttaaaatca caatggggtc
     2101 tggtttaaat tatattgaac aagaatctct tggaggaaaa tatctaacac atgatttgtc
     2161 aataaagata gcggatattt ctgaagagat aattcagcaa gcaatattat ctgctatgag
     2221 catatataaa ttttcgataa cagatgattt aatgagtatg gctgtaaatg aactcataaa
     2281 actgaccaaa atagagaata atgtagacct gaataaattc actactatat gcacagacgt
     2341 tctatccccc ctcgtcacca gacataataa agaaaaaaaa caaacgacat tctacccttc
     2401 tcaaaaatcc cctttttaat tttcattgaa aaaatggcaa ttgatactat gaatccaggt
     2461 catacacatt aaggcattta tgtaatgtcg taattaagat aactaataag gtgaatatta
     2521 gtaaaacaac taatgatg
//
LOCUS       DAZPLI010000295         2515 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZPLI010000295 DAZPLI010000000
VERSION     DAZPLI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.298"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(209..466)
                     /locus_tag="ACHUU3_004345"
     CDS             complement(209..466)
                     /locus_tag="ACHUU3_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084224.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(463..960)
                     /locus_tag="ACHUU3_004346"
     CDS             complement(463..960)
                     /locus_tag="ACHUU3_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFR2084225.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1162..1620)
                     /locus_tag="ACHUU3_004347"
     CDS             complement(1162..1620)
                     /locus_tag="ACHUU3_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR2084226.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1617..2114)
                     /locus_tag="ACHUU3_004348"
     CDS             complement(1617..2114)
                     /locus_tag="ACHUU3_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR2084227.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2114..2329)
                     /gene="essD"
                     /locus_tag="ACHUU3_004349"
     CDS             complement(2114..2329)
                     /gene="essD"
                     /locus_tag="ACHUU3_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFR2084228.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          593 a          661 c          579 g          682 t
ORIGIN      
        1 tttggctgaa attgggatgt ctttccatca gtccgccacc acaaagaatc ttttttgcca
       61 taaggcagga ggttcatctt tcagtggctg ccggtgttat ttccccactt actggcttgg
      121 gttgtttcgt ggtactgccg ttaactggtg gcccagaata aattccggtt tcattatcaa
      181 gcccacccgt aaatgggctt tgtaatggct acttcgcttt tgcttccgct cgcttacgcc
      241 ggcgctcttc tttcctctcg gcttttgcca tgtccatgaa tgcctgcatg atcgagttcc
      301 gcatcatgta gctaacaaag tgatgattga cacagccgtt gaggcgcagc tgctcgccaa
      361 actcatccac cgaggccaat gcttccatca tgcccttctc gcctttcatg aactctgaga
      421 agtcgcgccc cgctctggag gcgcattcaa taacatgatc actcatcccg gaagcccggg
      481 gatcgtaatc tgcagctggt tagccaggga gttaatctca gcgaccaaca ctggcttcgt
      541 atagcgccat gctgccagcc cttgtccgca gaagctcgcc atgtctttct tctggtcaaa
      601 ttcatgacat ttcatgttga gctgcgcact taagctgttg cgatgctgaa gttctccggt
      661 gaagtagtca tcgaggactt tataggccgc gtacttgaac ccggggttta accaagccgc
      721 ataatcgtaa gcaacaaact tcccgccata tgttccaccg tgtacaccgc gctcagtaaa
      781 aaccacagat tcgtggtttt tctccagctc ggctaagaac tctttggtct gcttgtttcg
      841 caggtagtgg taaggcgatt cagattcact tttaccactg gctttccaca tatcagtgag
      901 gcagatcatg ccatcttcac cgatacgaat tggttgattg aagagggtta atgatttcat
      961 agcgtgtacc tactctttga aatgaacctt tgccgcacag gaaaccagcc caccgaggct
     1021 cgccagcact aactggtatc ctcaaaggcc cattccaaag gggcaggttc ggtgtaaaaa
     1081 acatgcgttg cggtacgcat ttattgcaaa aagccccgca tcgcgaggct cattaaatgg
     1141 actttgtgat ttgcaaaaaa attatttcag gcattgcgtc ctgatgtatt cctgcaggta
     1201 gttaacctgc gcggttatct tgtcgattcc acttcggaga cggtaataat tgagttcagc
     1261 atctgctgta agtcctgggc tttctccatc gcccatgccg ctggctccgg tcgttgactt
     1321 tgcacaggtg gcggcgactt gcaggcgctt acgcccagca gaaacatcag cacggagact
     1381 ttcgatagtc gcgttagcat cagcaagctc ctttatgtat cttgcgtcga gttctgctac
     1441 atcacgttga cgcttccgca tgtcagcgat ggtggcgttc gccttctcca gttcactggc
     1501 cttgttatcg cgctgctctt tgtaggcgat tgcgttatca cggtaatgat taacagccaa
     1561 tgacaggcag acgatgatgc agataaccag agcggagata atcgcggtta ctctgctcat
     1621 tgtttccccc acaaacagac ttcacgctca atctcacggc gagtcatcag tcctttccat
     1681 tgattaccgc cagcgtatgt ccagcgccgt agctgatcac atgcgccttt aatatcaccc
     1741 tggtttattt tgcgaagaag cgtcgatgtt ctgaaattgc cagcacccac gttgtagacg
     1801 aacgagtaaa gagcgcagcg cgttgtttcc ggtatatcga ctttgatgta cgggttaatt
     1861 tgtctggcga cagtggcaag gtctttattc aggagggctt tgcactctgc ttcggtatac
     1921 gttttaccgg gaatgatgtc ttttcctgta tgcccgtaac atacagtcca tacaccaact
     1981 atgtctttgt aaggattatg tctcacacct tccagaccat cgttaccacc tgggccagtg
     2041 attaacacag atgctatagc aatagccccg ccaccaatag cagcagcaac agcttttcgt
     2101 aatgatggag gcattatcca cctctcgcag ccttgcgctt atcttcttta atcttgaaat
     2161 aaaggtttgt caggtacgtc agcaggccaa ataccaggct acccagcact ccaattgccg
     2221 cccactgtga gggcgtgact ttatctagca gctgtaaaaa ccagtacccg gcactacctg
     2281 ctgaggtgcc ataggcgaca cccgttgtta acttatccat ggatttcata accccacctc
     2341 gcagacaaag cgggtgtaaa ttgagggaat actacgaaac gtaacagact cggagtcagt
     2401 gaataactca ggtattgggt tatcagctaa tatcgagact caaaaaatgg aaaaacccgc
     2461 tcgacggcgg gtttaagctg tgtgacgaag taaccactct taacagcata accaa
//
LOCUS       DAZPLI010000296         2446 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZPLI010000296 DAZPLI010000000
VERSION     DAZPLI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.299"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(107..1525)
                     /gene="phrB"
                     /locus_tag="ACHUU3_004350"
     CDS             complement(107..1525)
                     /gene="phrB"
                     /locus_tag="ACHUU3_004350"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFR2084229.1"
                     /translation="MTTHLVWFRQALRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(1522..2031)
                     /locus_tag="ACHUU3_004351"
     CDS             complement(1522..2031)
                     /locus_tag="ACHUU3_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR2084230.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT          622 a          630 c          560 g          634 t
ORIGIN      
        1 caggcctacg tatttcctgc aatttattga atttgcacaa atttgtaggc tggataaggc
       61 gttcacgccg catccggcat ctgggctcga ttgcctgacg cgtctgttat ttccccttcc
      121 gcgccgcctc atacgctgcc aacgtttgta ctctcgcctc tttgtgctcg actatcggtt
      181 gcggataatc cagcttcaca cctgctttct gcgcccactt ccacggctca tgcaccgatt
      241 tccccggtac attgcgtagt tccggtagcc actggcggat aaactcacct tcgagatcaa
      301 atttctcgcc ctgggttgtc gggttgaaaa tacgaaaata cggcgctgca tcggttccgg
      361 ttgaagcggc ccactgccag ccaccgttat tggctgccaa atcaccatca atcagctgcg
      421 acatgaaata tcgctcgcct tcgcgccagt cgatcaataa atctttcacc agaaaactgg
      481 ctgtaatcat ccgtagcctg ttatgcatcc agccagtgct gttaagctga cgcatagcgg
      541 catcaacaat cgggtatccc gttttgcctt cctgccaggc ctgtaaatgt gcgggattgc
      601 tctgccactg tacacgatcc gtccaggcaa taattggacg atgtttacac aacgaggggt
      661 gatacgttat caggtgacgg taaaactcgc gccagatcag ctcattaagc cagacactac
      721 cgaccccacc gtccagcgcc tgcggctgtt cagccaacaa gcgatgcaag cactggcgag
      781 gcgataaccc gcccgttgcc aggctggccg acaaacggct ggtgccttcc actgccggaa
      841 aatctcgttg ttgctcatat tctccggcac cgttctggca aaactggcgt aattgcgcaa
      901 tcgccgcttt ttcttccacc ggaaaatgcg cagtatcgaa agactgacga ggataattca
      961 gcgtaatgga tggcgagggc tctatcgatc cgctactacg aacttttggc gcagcgacgc
     1021 actccggcat cccttcccgc agccgtttca gccaggcatt cttaaaaggc gtaaagactt
     1081 tgtacatctc gtgattaccg gtcatcaccg cgccaggcgg caggatcacg ctgtcatcaa
     1141 atccttcaca caccacgtta cgcagcgctc tttcaacttc cacatcccgc gcccgctcat
     1201 tcacttcata ctgatagtta taaaacaggt gggtaacgct gttttccgcg cacacctgtt
     1261 taactatttc gacactggcg acaaagtcat ccacttcacg gaacaataaa ggaatacctt
     1321 tttccgcaag cgctatttgt agcccattca gttgagcatt gataagttca gcctgacgcg
     1381 gcgacatgtt atgcgtcgcc cactggcgtg gtgtagcgat atacaacgcc agcacgcgtg
     1441 cagacgaatt gcggcaggcg gcagccagtg cgagattatc gtgcagacgt aaagcttggc
     1501 gaaaccagac cagatgggta gtcataaaac tcctgaatgg cgcaagttaa tcacacgcgg
     1561 ccaaagttcg aaataacgct gccctgaaag ccaggcgtca tgataaagcg ccatatagtg
     1621 tttgatacgc atcagcggcg caagaagtgg ttgctcgcca cggcgataac tgtcgataag
     1681 cgcagccagc tgctggcgtt ctgtggaatc aatatgcggg cgaaaataac cctgaacgtg
     1741 catcaaaaca ttggtgtgat cgcgcgggtt ggcggggtga gaaagcaaaa caactacgcg
     1801 ttggcggtac tggttgtaat agtcatccag attttgccac tggtgaatat cagccacgaa
     1861 cgggccaagt ttgcggtact ccggctgaga atgcgccaga aagacgagtt tatagcggct
     1921 gtgataatcg agaagcgccc cgcgcgttaa gccatgttct ttcagtcgat gtaactcatg
     1981 aagggcaaag atacggatta gggtgctgtc atcaaatcgt tgtagattca ttactgctcc
     2041 tgaacggtat aaaggagtaa gtgtagacga tggtaaatga attttatggg gcagagtttt
     2101 tgcggggaag tattttgttg gtaaaaatat tatcaacttc atctgagtga gaattattct
     2161 actatttccc gagctgcatt cattttttgt attttttatt tttagaaaat aacagttctc
     2221 aaagcttaat tacctcacta ttaattgtat ttcaaactaa caatcgcact ccattttctc
     2281 tttttaaaaa acataaacac tgttaaaaca aatacttatt tcgttatctt aaatgctatt
     2341 tctacatcac tcgtccttat aatcaaatta ttcttattca tagctatttt tgcaggttta
     2401 aaatagcggc tttcaccaaa gtcagccatg aaaattagac tacaag
//
LOCUS       DAZPLI010000297         2437 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZPLI010000297 DAZPLI010000000
VERSION     DAZPLI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.300"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..458)
                     /locus_tag="ACHUU3_004352"
     CDS             complement(<1..458)
                     /locus_tag="ACHUU3_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR2084231.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTSDTALLKSFEAFREWVAIQAGFYTEHFYPDGSRGCRAKSIA"
     gene            complement(497..784)
                     /locus_tag="ACHUU3_004353"
     CDS             complement(497..784)
                     /locus_tag="ACHUU3_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084232.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(1034..1189)
                     /gene="hokD"
                     /locus_tag="ACHUU3_004354"
     CDS             complement(1034..1189)
                     /gene="hokD"
                     /locus_tag="ACHUU3_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR2084233.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1417..2082)
                     /locus_tag="ACHUU3_004355"
     CDS             complement(1417..2082)
                     /locus_tag="ACHUU3_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR2084234.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(2251..>2437)
                     /locus_tag="ACHUU3_004356"
     CDS             complement(2251..>2437)
                     /locus_tag="ACHUU3_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084235.1"
                     /translation="GAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQI
                     ERNAKARIRRATKSRTE"
BASE COUNT          607 a          639 c          543 g          648 t
ORIGIN      
        1 gcgatagatt tcgcccgaca cccacggcta ccgtccggat aaaaatgctc ggtgtaaaat
       61 ccggcctgaa tggctaccca ctcgcggaaa gcctcaaacg actttagcaa tgccgtatca
      121 ctggttctgc gtgtcgcaac tgtattcaga tattgctctg cggcttcgct cagagctggc
      181 gtatgttccc ggcctactga ttcacacagg taatcaacga aaccggacac cagttttcgt
      241 tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat cgaaacccag ttgcaggagt
      301 ttgaaaaaac gcttgtggaa tgcgtagtta cacacacgct taaagtctgc gtgtatccac
      361 tcacctattt tgatttgatg cagaaaatcg caactctccg gcgtcgccgg gagaagtaat
      421 ccggaagagg tttgtttgac cagttgtata tgtgccatcg gctttctccg gtggcacggt
      481 gttacacagc aggagttcaa tcctgctcaa gattgtagat gagtttattc ttcagcaaaa
      541 gcagaaaacc agccttaaaa ccaatctctt tcaaaacccg taatgatgtg acaaattcgt
      601 cctcacgcaa aataaaaccg tccgtcagaa gtccattaca aaaataaaat aacacagcac
      661 cgctcttcct ttgttgagat tgcaaacatc taatgcggca atggctgaca atcgctccat
      721 tctcaacgcg cacagcatag aggccatttt cactaaaaat ttcacgcaat tcttcgattt
      781 tcatcttcag aatccttcca gataaatagc tctcccctgt tcggggtcca tccctcttct
      841 ccctgcgcgc tacttaagtg agtcgattct atctgcgaag gtgcgcgaat caaattcacc
      901 ggaaataaac aacaaaaaac ccgccgaaac gctgagagat cacagcgatg ggcgggttaa
      961 gtgcgggtgc gttgaggatg cctgacacat cagaggtggg cggggatggg atcagctccc
     1021 cgcccggtca ctcttacttc ctggattcgt agtctacgaa gacagcgacc tccgtctggc
     1081 cggttcggat tcgtacctca cagaggtctt tcctcgttac cagtgccgtc actatgacgg
     1141 ttaaacagat gacgatcagg gcgattaaca tcgccttttg ctgcttcata gcctgcttct
     1201 ccttgccttt cggcatgtaa gaggctaacc tacatgtgca aagcatgaaa ttggcctcag
     1261 attaatgtta agcgtcttgc cggacgcgta atgttaactg gggcttttct ctatctgcct
     1321 tttggtgttc atgcctgaag cagatagcct caagcacccg cagtcattct acttacctcg
     1381 ccaatatgaa atcaatcaga aaggtgcccc ataaaatcac tccttctctt ctttaccgta
     1441 gtggagttga ccaattttga taagagggcg tccctgagat ttgcggtgta gattggtatc
     1501 gcgcagagaa tacacacagc cacaatattc ctgctgatag aatttttcgc gcttgctgat
     1561 ttcaatcata cgggacgagc cgccctgctt gcgccagtta taatcccagt acaccatgcc
     1621 cggataatgc gcgacggctc gccgcccaca gtcgttaacc tgctgcatat ttttccagcg
     1681 tgaaatgccc agtgaactgc tgatcacact gaaaccattt tcagcggcgt acagcgctgt
     1741 ccgctcaaaa cgcatgtcaa aacacatggt gcaacggatc cctcgttcgg gctcccattc
     1801 cattcctttg gctcgttcaa accagttgtc tgtgtcgtaa tcagcatcaa taaacggcac
     1861 gccgtgttgt tcagcaaagc gaatattctc atccttacga attaaatact ctttctgagg
     1921 atgaatgttc gggttgtaga aaaagatggt gtagtcgatt cccgaggcct gaagcgcctc
     1981 catcacctca ccggaacatg gagcacagca agagtgcagt agtagtttgt ttgccccgtt
     2041 tgggagctcc aatttgggcc gtttgaaatc agcaactgtc ataaatatgt ttattggggt
     2101 cataaaaata tcaaagagtg tagcattaga acggggctat cggaaacaga tgtgtgactg
     2161 ctccccgccc tttcgggtgg tctcctgatg atttgagggt gcagaaatcc ctccggttaa
     2221 ggattaaatt tttaacagtg ctaaatttaa ttattcagtt ctggattttg tcgccctgcg
     2281 tatccgcgct ttcgcgttac gctcaatctg tatcagcttt tctatatttc tccgcctttc
     2341 ctgttcctcc tggcgcaata gccttacatc atctgccagt ctggtttctc tttttgccac
     2401 agagagcatc cagtcaaacg gctccacaac cgcaccg
//
LOCUS       DAZPLI010000298         2360 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZPLI010000298 DAZPLI010000000
VERSION     DAZPLI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.301"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(1..806)
                     /locus_tag="ACHUU3_004357"
                     /pseudo
     CDS             complement(1..806)
                     /locus_tag="ACHUU3_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(859..1455)
                     /locus_tag="ACHUU3_004358"
     CDS             complement(859..1455)
                     /locus_tag="ACHUU3_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR2084236.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1482..2084)
                     /gene="yadL"
                     /locus_tag="ACHUU3_004359"
     CDS             complement(1482..2084)
                     /gene="yadL"
                     /locus_tag="ACHUU3_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFR2084237.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(2099..>2360)
                     /locus_tag="ACHUU3_004360"
     CDS             complement(2099..>2360)
                     /locus_tag="ACHUU3_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2084238.1"
                     /translation="SSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIR
                     WSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          664 a          507 c          471 g          718 t
ORIGIN      
        1 actaagcata gcggtaaaac aagtgggatt agtcaacttg acattgagca gatcgaaatc
       61 gattttgata aaataactat gctcatttat accttggcct gcgccatagg ctttaaatga
      121 atacagataa tttgatgagc tggataattt gatctgttga gttactgttg gatcaaagtt
      181 tgtatctgta tagaattcaa ttgtaagggt ctgataaata ccacctacag cccagtattt
      241 acgggtgcta ttcgcattat tgcaactcca atataacacg tcttcgcttg gattatacca
      301 cgaaaaatat tgttctgcag agtcaccaat gtatatccca ggtgaactaa cgttagttac
      361 ggtaccgtaa gctgaccaca tgtttgaaat taataatgtg taatacaaac caggaactga
      421 tgtattaaat aatttatggg agccatacat tttacctgag tcaaccattg cattttcaat
      481 acgcattgcg ctaccacttg tgctatcact tctgttgcaa taaagaaaac caccaccaga
      541 agcggtatct aactgactcc actgtaattt tacacctgta ggtccaagct gcgtcggttt
      601 gtagaaaact aaattatttg tacttgttgc atatctgaat atgacagtag catcgccatt
      661 cggccccgtt tctctccatt tttgatctga cggaccaacg ccgtaactat tcccaacaat
      721 atatcctacg ttatcatcag tgccagcact cgcggtataa caactcaagc acagaattaa
      781 aaaaaatgtc tgaagaattt cttcattatc actccaccat ctactgctgt aacagtcgca
      841 gttatttgtt ttgatctatt attcgtagta cacatcaact aatatatgac tcattacttg
      901 gccagatatg acatccagcg cgggatcgat tttttgcatt ctggcataaa atttccatga
      961 agaattatgc agggcagaag tcattactgg atacacggaa cgagaatttc ctgctgaact
     1021 aataacattg aatatattgg tcgggtcctg aatggaaaag agaacgacac cgacattttt
     1081 agccccggtt gcgacttcat tggcaaatat ttgccttgaa cctgcagcca tgacaccgct
     1141 aagggctcta aaatcaatat gtaactgatt aagcacatcg ccagttccct gtaatgcaca
     1201 gtctgatact gtgacagtga actcttttcc acctggttga tagctttcag gagtaacatt
     1261 atctgaaaaa tatcccaccc caacgacgcc aagatcaata ttaccctggt tactgattcc
     1321 gcttttgcaa gtgctgtttt ttacattggc aacaaggtca atatcctgcc cagcgatggc
     1381 tggataggta atgggaaact gtagtgacag aaacagaatt attctcttca tcagcttacg
     1441 ttgagtggga tgcatatata gttcctggat ctgtcagtct gttagttata ggttactgta
     1501 aatgtggcag gtgcagaaaa tttacccgca gtgacattgc ttacggtttt atttttttcg
     1561 acgaccagtc gagcactcat cggataataa acaaccgcgt tggctgcatt ggagatagtg
     1621 acctcttgag atgctggtgt cacacaacta aattgtgttg cgctccctgt tgccggagtt
     1681 tcggtgctcc agacttcaac ggcgacggca cttgctttat ctgcggctgt ggatccattt
     1741 gcgagccccg caccgtcatt agcagtacct tcgcatgtgg ctcgcttggt taattttatt
     1801 tgtgcttttt tattggggat acccgcacag tctttgaatt tgagtttgaa tgttttaact
     1861 ttggtcttgg cattgatttc agaaatatat acatcgccaa agtcaacaac agaaatgtcc
     1921 tgatcagagt catttactaa tgtagcggta caagtaccta tttctacggt agtaatcacg
     1981 gtcagcccaa tgctgtctgt tgccgcataa gagggtacgc tgaacaatga ggcagccaaa
     2041 acgacactgc tggataatcc ataacgtaaa tttttaaatg tcatcttaat tatccttctt
     2101 attgatagat gaaattaaac gttgccagtg cacgaaaatt ccccggtacg ccttgccctg
     2161 catctgtttc acgtagcgca acggtcattt caagaccctt atcgggctgc atctcgtctg
     2221 tgctccagcg tatcttttcc gcactgttag gtttaaggaa agtggcatca tcagtagtcc
     2281 gttttttgaa acccataccg atattacttg tcgtcgaaga tgaatcacca gactgcggta
     2341 taataagctt aggtgagctt
//
LOCUS       DAZPLI010000299         2356 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZPLI010000299 DAZPLI010000000
VERSION     DAZPLI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.302"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            48..266
                     /locus_tag="ACHUU3_004361"
     CDS             48..266
                     /locus_tag="ACHUU3_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR2084239.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            268..633
                     /locus_tag="ACHUU3_004362"
     CDS             268..633
                     /locus_tag="ACHUU3_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR2084240.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            630..1295
                     /locus_tag="ACHUU3_004363"
     CDS             630..1295
                     /locus_tag="ACHUU3_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084241.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1295..1660
                     /locus_tag="ACHUU3_004364"
     CDS             1295..1660
                     /locus_tag="ACHUU3_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR2084242.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLNDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2115..2270
                     /locus_tag="ACHUU3_004365"
     CDS             2115..2270
                     /locus_tag="ACHUU3_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR2084243.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRIGQTEVAVF
                     VDYESRE"
BASE COUNT          636 a          505 c          632 g          583 t
ORIGIN      
        1 tctgcaccct caaatcatca ggagaccgct cgaaagggcg gtaatgaatg gttacattat
       61 ttagaaaaaa atatccgcga aagagtagaa caacagaatt cctgtttctc attctgttta
      121 tcgtgttgat gacaccgata tccccgctaa tttttgtctg gataatcgga aaaataattg
      181 agccagttat tgaattgtat aacgacgtgg tatgggcgtc attcaacaca ctgcacaata
      241 aaattaatcc gtataaggaa agctgatatg gcactgacga aaaaacaacg tgcagaactg
      301 cgcatgaagt tcggtggtcg ctgcgcttat tgcggctgcg aacttggcga aaagtggcat
      361 gcagaccatg taaaaccggt cattcgtttt gatggaaata tgcttcacca ggaacgtgac
      421 gatatatcca acatggtacc agcatgccac ccatgcaatc tgcacaagca ttgcagtagc
      481 ctggaagatt atcgacgaat tatcagtgat ggtcgtcgtg aattccttgc gtccgggaaa
      541 ggcaaagcgc tggttcgtat gggattggtt gaaatgaaat ctgacccggt tgtgttctgg
      601 tttgaaaaat atcaagaagg ggctacggca tgaccactat taccaaagag cgactgcaat
      661 ggctggctaa catttctggc cgcgatgata ttgacgatat agacggcggt gaaattcgtg
      721 aactggcgct tatcgctctg gcatcactgg aagcagagcc tgtagcggaa tgcattgttg
      781 aagatggggg catgtgtatt gacgggttcg gtgagtatgt gggtcactcg ctgcctgatg
      841 gaatgcatga gctttatgct gccccgcgaa tgcgacaacc tgatggatgg aaagccgtag
      901 ctgtagcgtg gaaggtgacg tttactcaag ttgaccagga atctaatacg ttcactgcta
      961 tatattttga caaagcggaa gttgagtgtt gggtacgact gcataaagca tgtgattttc
     1021 gggcagatat aacaccgctt tacgcagcgc cggcagtgcc ggttgcagta aacgacgaca
     1081 tggcttacgc attccatcac gcactgtcag attcatcgct tggctctgat gaaatcgaag
     1141 aaattaaaac cggtttgcgt gctgcctttg ccaacgtcac tatccaacca gagccgatag
     1201 tgccggatga aatcgggcca aacgatagta atacgtttga ttatgttgat ggctggaacg
     1261 cctgccgcgc tgccatgctt aagggagata aataatgatt aatcgaacca aactggagca
     1321 cattctcgaa tatgccaggc agcagaaatg tattgggcaa ctttgtaaaa ttccaccagg
     1381 agatatggtt gaaatcgtgg aaatggccat gcgtaaggct ggcaactctc cggtagctcc
     1441 ggctggctgg ataagctgta gtgatgcagt tcctgctgaa tattgcgatg tgattcttct
     1501 caatgatctc gggaatgtat tccccggttc ctgggataag gttttttgcc ccactcgtgg
     1561 cgggaataag atggcttttg tggacaaaga cggcgttgaa gtagagagct caactcactg
     1621 gatgccgcta ccggaaccac cgcaggaggt gaatcaatga cctggcctga ggcattcacc
     1681 acggtaggaa ttgcgatggc ggtggcgctg gtggtgtatt cgatttgccg ttggggttaa
     1741 caaacaaaaa ccccggattg atggtccggg gtttttgaag gaaacaaaac agaaacagca
     1801 attgccgtta cctgttgtta ccatggcaag taaacgtatc tcaggcgagc gcattgcgcc
     1861 gttctgacgc agataaacta gcctggatag atggtgctgg caataaaaaa tagcgttttc
     1921 ttatcggtgt cggtaagatt gctgcgggtg cttgaggctg tctgcctcgg gtatgtcact
     1981 gtaaggcaga cagagaaaag ccccagttaa cattacgcgt cctgcaagac gcttaacatt
     2041 aatctgaggc ccaatctatg tctcacaaat gtaggttagc ctcttacgtg ccgaaaggca
     2101 aggagaagca ggctatgaag cagcaaaagg cgatgttaat cgccctgatc gtcatctgtt
     2161 taaccgtcat tgtgacggca ctggtaacga ggaaagacct ctgcgaggta cgaatccgaa
     2221 tcggccagac ggaggtcgct gttttcgtag actacgaatc tagagagtaa gagtgaccag
     2281 gcgggagagt aatctcccgc cacctttgat gtgtcaggca tcctcaacgc acccgcactt
     2341 aacccgtttc ggcggt
//
LOCUS       DAZPLI010000300         2341 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZPLI010000300 DAZPLI010000000
VERSION     DAZPLI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.303"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            574..2241
                     /gene="ltrA"
                     /locus_tag="ACHUU3_004366"
     CDS             574..2241
                     /gene="ltrA"
                     /locus_tag="ACHUU3_004366"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR2084244.1"
                     /translation="MNTHISVSTIPHPTGWHTINWKACHARVRKLQLRIAKATRQQQW
                     RQVRELQRILTRSFSGKAVAVRRVTENTGKRTPGIDGKIWHTPKEKWEGICSLNLCGY
                     RPQPLRRIYIPKSNGKTRPLGIPTMRDRAMQALWLLALEPVSETTADHNSYGFRPMRS
                     THDAIESIFLRMSQKVSPKWILEGDIKGCFDNISHDWLLSHIPLDRKLLKKWLKAGYM
                     ERGVFNHTNSGTPQGGIISPVLANMALDGLEKELMQTFRKSGYHSAKHQVNYVRYADD
                     FICSGSSRELLENEVRPLIAAFMRERGLELSEEKTAITHIDKGFDFLGQNVRKYNGKM
                     LIKPSKKNLKNFLCKVREIIKRNPTLPAWKLIGQLNPVIRGWATYHRHVVAKETFNYV
                     DTQIWRAIWRWCVRRHPRKGLRWIAGRYFSFEGRRWIFKAITPEGKILTLFRAMETPI
                     KRHIKIKGEATPYTPGMEIYFERRLDLIWKGKSKKMKTVVQLWKRQGKHCPQCGQPIT
                     NQTGWNIHHRIRKVMGGSDELTNLELLHPNCHRQLHSREAGAHRKHL"
BASE COUNT          689 a          586 c          638 g          428 t
ORIGIN      
        1 cgtattgttt tacattgata aaaccagcta tcccattagc tgtagcctac cgggacaggc
       61 gctgccggca acggcggggt ctgaagctcc cgggacaacg agagccgggc gtcagcccgg
      121 gtaaagcccc gtgaggggct ttaccttgct gcaagcatca tgcggcagga gagcaaggct
      181 ggtcagaagg gttaacacat gtgaactgtt gataaacacc gttatcggca acaggccaaa
      241 gatgctgaca ggcctgaacc aaaaggtacg gggtccggtt gctacctcgc gcacagggag
      301 cacatgggcg cagagccccc cggtgaagag acagaacccg ccctgaccct ctgtatgtta
      361 agcggaacct ggtaaacccg tatcgctgcc tcacaggcag gtgaaccgca aggaacactg
      421 ttagcggtgc gggcagagga ggacggaaaa agcgaatgcc cggctgtaat aaccgggata
      481 cggggcaggt tccccgacgc gaaagcgggc agacttccgt ctggtcttta attgcatgaa
      541 aagctgtaaa gcagtcaaaa ggatatcagc gagatgaata cacacatcag tgtgtccacg
      601 ataccccacc cgacaggctg gcacaccata aactggaaag catgccatgc ccgggtgcga
      661 aagctacagt tacggattgc aaaggcaacc cgacagcaac aatggcggca ggtcagagaa
      721 ctacagcgca tcctcacccg ctcattctcc ggcaaagccg tggctgtcag gcgtgtaact
      781 gaaaataccg gtaaaaggac acccggtatt gacggaaaaa tatggcacac accgaaggag
      841 aaatgggaag ggatatgcag cctgaatctg tgtggttaca ggccacagcc actacggcga
      901 atatatatcc caaaatcaaa cggcaaaacg cgaccgctgg gcatacccac aatgagggac
      961 cgggccatgc aggcactgtg gctgctggct ctggaacccg tatccgagac cactgctgac
     1021 cacaacagct atggtttcag accaatgcgc agcacgcatg acgccattga gtccattttt
     1081 ctgcggatga gccagaaagt atcgcccaaa tggatactgg agggcgatat taaagggtgt
     1141 ttcgataaca tcagccatga ctggctgctt tcccatattc cgctggacag gaaacttctt
     1201 aaaaaatggt tgaaagcagg ttacatggaa agaggcgtgt ttaaccatac gaacagcggg
     1261 acaccgcagg gaggcatcat atcccctgtt ctcgccaaca tggctctgga cggactggag
     1321 aaggaactga tgcaaacgtt caggaaaagt ggttatcaca gtgcaaagca ccaggtgaat
     1381 tatgtgagat atgctgatga tttcatctgt tcaggcagct cccgtgaatt gctggaaaat
     1441 gaggtcagac cactgattgc agctttcatg cgcgaacgag ggctggaact gtcggaagaa
     1501 aaaaccgcga tcacacatat agataaaggc ttcgactttt tagggcagaa cgtcaggaag
     1561 tacaacggta aaatgctgat taaaccgtcg aagaaaaacc tgaagaactt cctctgtaaa
     1621 gtcagagaaa taattaaacg caatccgacg ctcccggcat ggaaactgat aggccagcta
     1681 aacccggtga tccggggatg ggctacctat caccgacacg tcgtggcgaa agaaacattt
     1741 aattatgtcg acacccaaat ctggcgagct atatggcgat ggtgtgtaag aaggcacccc
     1801 cgaaaaggat tgcggtggat cgccggacga tacttctcct ttgaaggcag gcgctggatc
     1861 tttaaagcca taaccccgga agggaaaatt ctgactctct tccgggcaat ggaaacaccg
     1921 ataaagcgcc atataaaaat caaaggcgaa gcaacaccat acacccccgg aatggaaatc
     1981 tactttgaac gtcgtctgga tctaatctgg aaaggcaaat caaagaagat gaagacagta
     2041 gttcaactct ggaagaggca ggggaaacac tgtccgcagt gcggacaacc cataaccaac
     2101 cagaccggat ggaatataca tcaccggatc aggaaagtta tgggcggcag tgacgaactg
     2161 accaatctcg agttgttaca tccgaactgc caccgacagt tacacagcag ggaagccggt
     2221 gctcaccgaa agcacttata aaggcttgag cggagtgcgg ggaaactcgc acgctccgtt
     2281 cttagggggc ggcggcgcag caatgtgtcg ctgctacccg acccggcaga cagaacaaca
     2341 c
//
LOCUS       DAZPLI010000301         2325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZPLI010000301 DAZPLI010000000
VERSION     DAZPLI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.304"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(187..1191)
                     /gene="argF"
                     /locus_tag="ACHUU3_004367"
     CDS             complement(187..1191)
                     /gene="argF"
                     /locus_tag="ACHUU3_004367"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR2084245.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1353..1769
                     /gene="rraB"
                     /locus_tag="ACHUU3_004368"
     CDS             1353..1769
                     /gene="rraB"
                     /locus_tag="ACHUU3_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR2084246.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1815..2318)
                     /locus_tag="ACHUU3_004369"
     CDS             complement(1815..2318)
                     /locus_tag="ACHUU3_004369"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR2084247.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
BASE COUNT          603 a          566 c          562 g          594 t
ORIGIN      
        1 cctccggaat ggcaaaatca atatatatgg atatattaat gaagaacagt taggaaaatt
       61 aaatcctccg gaagatttac gtatagcaat aaaaaaattg gggtggaata attgaactat
      121 cactgcataa aataatgtga cgccgggatg tttttgtatt tcccggcatc tttatagcga
      181 tatcaattat ttactgagcg tcgcgaccat caccgctttg atggtatgca tgcggttttc
      241 cgcctgatca aatacaatgc tggcggcaga ttcgaagacc tcatcagtca cttccattcc
      301 gccatgtagg ccaaactctt ccgccatttt tttgccaagc gtcgtttggt cgtcatgaaa
      361 cgcgggcagg cagtggagga atttgacctc cgggttaccg gtcaactgca tcatcttgct
      421 gttcacttga taatcacgca gcaatgcaat gcgttcagcc catttctctt ttgcttcacc
      481 catcgacacc catacatcgg tatagataaa gtcagcacct tcaactcctt tagctacatc
      541 ttccgtcagc gtaatattcc cgccattttg ttgtgccagg gcgcggcatt ccgtaaccag
      601 cgcagcttcc ggccagcatg cctgtggcgc gaccagacgc aaatccagac cggtaagcgc
      661 tgcagcttcg agcatcgaat tgcccatgtt gttacgtgcg tcacctgcat agactagcgt
      721 catttcgttg aacgttttgc cgggcaaatg ctcctgcatg gtgaggagat ccgccagcag
      781 ctgcgtgggg tgaaactcat tggtcaggcc gttccatacc ggcacgccag cgtattccgc
      841 cagtgtttcg acaatctcct gaccatagcc gcgatactga ataccgtcat acatgcgacc
      901 aagcacgcgg gcagtgtctt taatcgactc tttatgacca atttggctgc cgcttgggcc
      961 gagataagta acacgagccc cctggtcata tgcggcaact tcgaaagagc atcgggtacg
     1021 ggtcgagtct ttttcgaaga tgagcgcgat gtttttacca gtgagtttgg cttcttcttt
     1081 accgcttttc ttatcggctt tcagcttcgc ggctaactgc agcaggctgt tgagttcagc
     1141 tggcgtgaaa tcgagtaatt tcaggaaatg cttatgataa aacccggaca taggtccctc
     1201 ctgtggctaa cgccttaatg aattaaaatt caatttatat ggatgattat tcatttgcaa
     1261 gtctaaagca taaatcttta tcacaaaggt ggaggcaatg tcagtggtgt gtgacaataa
     1321 gagtatcggc aggacattaa gaggaatgag ccatggcaaa cccggaacaa ctggaagaac
     1381 agcgtgaaga aacacgtttg attattgaag aattactgga agatggcagc gatccggacg
     1441 cgttgtacac catcgaacac catctttccg cagacgatct ggaaactctg gaaaaagcgg
     1501 cagttgaagc gtttaaactc ggttacgaag tgaccgatcc agaagagctg gaagttgaag
     1561 atggtgatat cgtgatttgc tgcgacatcc tcagcgagtg cgcgttgaat gccgatctga
     1621 tcgatgccca ggttgaacaa ctgatgacgc tggcggagaa atttgacgtt gagtacgacg
     1681 gttggggcac ttactttgaa gatcctaacg gcgaagatgg cgacgatgaa gattttgtcg
     1741 atgaagacga tgacggggtt cgccactaat taattgatta aaatgcccga tagcacatac
     1801 tagcgggcat ttttttagag ttcgcgtagc atccgcactt cacaatcgac atggcccgtg
     1861 cagccaagcg catagtcgat atgctcaaag cccaaatgct cataaagcgc aatggcttcc
     1921 tttaaaaaag cggtcgtttc cagatagcag cgtttgaagc ccatctctcg tgcctgctcc
     1981 atcgccatta aggccagttt tttcgctagc cctttaccgc gaatagccgg aagaaaatac
     2041 atcttttgca gttcgcaaat gtccgactca ctcccggtta atggcgcaat cccaccgccg
     2101 ccgaccactt caccctcgta ctcaaccacc caatatgcat ggccaggctg actatacact
     2161 tgatacagct cgtcaagatt cggatcggcg acggtgtagc ctttatcagc ggtaagaccg
     2221 tattcggcgg atacctgacg aatgacgcgg gcgatagcag gattatccag cagcgtcagg
     2281 cgacgcatta caggtgattg cggcgcaatg ttattcatag tttaa
//
LOCUS       DAZPLI010000302         2269 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZPLI010000302 DAZPLI010000000
VERSION     DAZPLI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.305"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(32..1396)
                     /locus_tag="ACHUU3_004370"
                     /pseudo
     CDS             complement(32..1396)
                     /locus_tag="ACHUU3_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
     gene            complement(1461..1580)
                     /locus_tag="ACHUU3_004371"
     CDS             complement(1461..1580)
                     /locus_tag="ACHUU3_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR2084248.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1804..1962)
                     /locus_tag="ACHUU3_004372"
                     /pseudo
     CDS             complement(1804..1962)
                     /locus_tag="ACHUU3_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          584 a          553 c          449 g          683 t
ORIGIN      
        1 ctggttgtct ggagattcag ggggccagtc tagcaactaa agcactgccc gtctcctggt
       61 taaccccatg tgctataccg ccggtataga caatcagcga gcctccggcg ctaatattgc
      121 tgccagttgc cgtaccatct ttctcaacaa cctgccggct cccggtggaa atatttgtcg
      181 tgtcagcttt cccgccgctc ttgatatttt gtgttccgct gttgatattg gtacccgtgg
      241 caataccatg attattaata ttctgtgtac caccattgat tatggtattg gtcgcgtttc
      301 tggtaacatc cattaccccg ccgttatcta ttcgggtcgc atcagcttta gcattcgtta
      361 aaactgacat tgttccttta tctttaataa tcgtcttatt tgccgaacca tatgcgttta
      421 tgtctaaatg accaccgttt tccagcaaca cattgtctgc cacgtgattg tggatggaga
      481 atgcaccttc actattcgta ccgctcaccg tcgtaccgtt agtgtttgtt tttaaaattg
      541 caccatcgtg ctgggtaaca tttgttgccg taccaccact aacatcaagc acgccaccgg
      601 aataaacttc aataacatcc gaggagctgg tgtaatcaac aatttgcgta ccaccagaat
      661 agatctgagt attttttgcc gttgacttat tattcagaga ctgagttcca ccttcaatcg
      721 tcgtgtccag cgcatggctt tcatatactc tttgctcacc gccattttta atggtggttg
      781 ttcctactgt gctctgttca acatactgtc gtccaccatt tatgattgtg ttcgttgcca
      841 tacttccttg cgtgatgtcc tgataaccgg acttatttat cgttgtacca tcaacatgcc
      901 cctgagttgt tactgtctgc cctccaccat caatgatggt tccattcgca gtccccccac
      961 ttatgtgtga attaccaccc ttaattgtcg ttccattgct gataccacct gtataaacgt
     1021 cctgattgcc actgtcgatt atcgtaccgg tggaaatgcc cccgtcatga attgtctgtc
     1081 ttcctccgtt aatggttgta ttattagact gccccatata attgttattc cttccaacat
     1141 cttgatatcc accagattca ataagacttc cattagatac tccgccataa acactctgct
     1201 ggccatggtt gataatatga gtgttatttg ttgtacctcg ttcatctact cgttggttgc
     1261 catctacagt ctcatcgttt accacaccaa taacattagt agtaaaggcc gccatcacgg
     1321 gcggggcata tatcaaggca gatatcaata aggaaagtac tgagcggcga caataatggg
     1381 gactggtcct gttcataaat ttcatcctct gaaaagtgaa tactgagtag cgtttaagcg
     1441 actttagctc tgctgcaaca tcagcccgca ggcaccagac cggggggttc atcctgaaga
     1501 gacagcgcaa gtgtattgtg ttcaccgctc atcaaagaca tcatgatgaa atgatgatgt
     1561 tccacataag aaagaggcat tttttaaaca cagtgcgctg aagtgtgatt ggataaaaaa
     1621 gtcaatctat tcaggaaata cgggcgtatt cttttctttc gacaatcagg ccgtcggcaa
     1681 aataaaatga tttacataat cgtttctgat gaatatcttc tgctcacata aaaatcacac
     1741 aataactttg agatcgcaga ttcttttact tttacagcat tcgtcccccc attgttgggc
     1801 aaatatagat tgggccagag cacgaaagtt aataccacgt ttgcacagct cctccaacag
     1861 cacgacaaga tgccacatac tgcgccccag tcggtgcggt ttacagacta cccgtgcgtc
     1921 caccgccgat aatgtcctga gcagtttttt cagtccggac ctcctgccac ggttttacgt
     1981 tgaagaggta accctgagca cgcagttctt cagtcaggcg tggtgcaccg taacgctgtt
     2041 attgctgggt aagatcaaaa aactttcagg cagctaagga aagttgaacc agacattaga
     2101 tgaaatattt caaccaatta cagcaccaat ttaaactcag taaatcacag cacagatcaa
     2161 ggggttacgt gaaagcgtag cccctttttc tttggtagtg gcagcaaaat ggttgtagtg
     2221 taaaaaataa tcccgtttaa tcaatcaata atacatattg tttcaatct
//
LOCUS       DAZPLI010000303         2246 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZPLI010000303 DAZPLI010000000
VERSION     DAZPLI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.306"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(381..515)
                     /locus_tag="ACHUU3_004373"
                     /pseudo
     CDS             complement(381..515)
                     /locus_tag="ACHUU3_004373"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(528..1349)
                     /locus_tag="ACHUU3_004374"
     CDS             complement(528..1349)
                     /locus_tag="ACHUU3_004374"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR2084249.1"
                     /translation="MFSHPVYLFLREFSLQDSRGGSLSKKAESLSSGKITIGTKEKVI
                     IINELRQCHPLSQLLVIADLPRSTFYYHVKRLNAPDPYQLVKQVILRIYHQHKGRYGY
                     RRIRLACRNEGILLNGKTIRKLMKELGISSLIRRKKYRAYRGEQGRTCNNLLKRQFYA
                     DRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEIVSYNLTERPLASMVKSMLLDAVEQ
                     LNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQSMSRRGNCLDNAAMESFFSTLK"
     gene            1338..2246
                     /locus_tag="ACHUU3_004375"
                     /pseudo
     CDS             1338..2246
                     /locus_tag="ACHUU3_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1618..1734
                     /locus_tag="ACHUU3_004375"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          567 a          506 c          533 g          640 t
ORIGIN      
        1 tgctgcagat taatcacgcc tgatctctgt cccggtattt tagcccgagg tatattctga
       61 aatatcattc atcatgaccg gcgtttatac aaacgccggt cattcgctaa cagcttcacc
      121 gttccttccc ccatccaggt tccgcatgag cgtgttgtaa tgttcataag cgtattatca
      181 ggattgtctg gaaaattccc gatactttgg actattactc ctgaacaatt cgaaattcag
      241 cagattaaag gtaagcgtta acggaggtaa gcgttaacgg aggtaagcgt aaaccgactg
      301 ccgtatgtag ccattaagcc agtattggta acgtaggtgc ccaccttttc aaatgatctg
      361 cgccccaaat cttggacatc ctatgaaaaa tgtttaagtc gatattgaac cggactcatg
      421 ccatcgagtt tctctttaat gcgatggtga ttgtagtatt caatgtaatc aattatatct
      481 tgctttagat gatgtaactt attgtattta tttaggtagt aacattctta ttttaaagta
      541 ctaaaaaagc tttccattgc tgcattatct aagcagttac cacgtcggga catgctttgt
      601 gttatgccat tatcactcaa ccaacgttgc caacgaggca tctgatattg ccacccctga
      661 tcactatgca acaagggctt atcgtcttta ttgagttgtt ctaccgcatc taatagcatg
      721 ctctttacca ttgatgctaa tgggcgctca gtaaggttat atgagactat ttctccatta
      781 tacaggtcca tgattggtga taaatagagc ttcctgccat cgaccttaaa ctctgtgaca
      841 tctgttaccc acttttgatt tgggcggtca gcataaaatt gtctcttcag taaattatta
      901 caagttcgcc cctgttctcc acgataggca cggtacttct tgcgcctgat aagtgagctg
      961 atacccagtt ccttcattaa ctttctgatg gttttaccat taagtaaaat gccctcgtta
     1021 cggcaggcaa gtctgatgcg tcgataacca taacgccctt tatgctgatg gtatattctc
     1081 aatattactt gtttaacaag ttgatatgga tctggcgcat taaggcgttt cacgtgatag
     1141 taaaatgtac tacgtggcaa atccgcgata accagtaatt gtgataatgg atgacattgc
     1201 cttaactcat taataatgat tactttttct tttgttccaa tcgtaatttt tcctgagcta
     1261 aggctttcag cttttttaga taatgaaccg ccccgggaat cctggagact aaactccctg
     1321 agaaagaggt aaacaggatg actaaaaata ctcgttttcc cccgaagtcc gtcaacgggc
     1381 agtccgtatg gttctggaaa gtcagggcga atatgactca caatgggcgg taatttgttc
     1441 cattactcca aagattggct gtacaccgga gactctgcgt gtctgggttc gccagcatga
     1501 gcgggatacc aggggcggtg atggagggct caccaccgct gaacgtcagc gtctgaaaga
     1561 gctggagcgt gagaatcgtg aactgcgctg cagtaacgat atccttcgcc aggcttccgc
     1621 ttattttgcg aaggcggagt tcgaccgcct ctggaaaaaa taatgccact gctggataag
     1681 ctgcgtgggc agtacggggt cggaccggta tgcagtgaac tgcatattgc cccgtcaacg
     1741 tattaccact gttagcaaca ggacatcatc ctgataaacg cagtgcccgt gcgcagcacg
     1801 atgactggct gaagagagag atacagcgcg tatacgatga aaatcatcag gtgtacggtg
     1861 tgcgtaaagt ctggcgccag ttgttacggg aaggaatcag ggtggccaga tgtacagtgg
     1921 cgcgcctcat ggcggttatg ggacttgtcg gtgttctccg gggtaaaaag gtccgcacta
     1981 ccgtcagccg gaaaaccgtt gccgcaggtg accgcgtaaa ccgtcagttc gtggcagaac
     2041 gtcctgacca gctgtgggtg gctgatttta cttacgtcag cacatggcag ggcttcgtct
     2101 atgtggcgtt catcattgat gtgtttgccg gatacatcgt gtggtgacga gtctcatcgt
     2161 ctatggaaac gacattcgtg ctggatgcac tggagcaggc gttgtgggcc cgtcgtccgt
     2221 ctggcaccat ccattacagc gataaa
//
LOCUS       DAZPLI010000304         2202 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZPLI010000304 DAZPLI010000000
VERSION     DAZPLI010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.307"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            58..609
                     /locus_tag="ACHUU3_004376"
     CDS             58..609
                     /locus_tag="ACHUU3_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR2084250.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            669..>2202
                     /locus_tag="ACHUU3_004377"
     CDS             669..>2202
                     /locus_tag="ACHUU3_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR2084251.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYMNYQNEKESYIVTFNQYLESLRLN
                     TYV"
BASE COUNT          675 a          455 c          439 g          633 t
ORIGIN      
        1 cttaaataac agttactaat cattcatttt ctacttatat ttatataaag gaatattatg
       61 tctgctttta aaaagtcact tcttgttgct ggcgtagcaa tgattctttc caataacgta
      121 tttgctgacg aaggtcacgg cattgttaaa tttaaaggtg aagttatctc tgctccatgt
      181 tcaattaaac caggtgacga agacttaact gtaaatctgg gtgaagtagc agataccgtt
      241 ctgaaaagcg accagaaatc tttggctgag cctttcacta ttcacctgca agactgcatg
      301 ttgagccagg gtggaaccac ttattcaaaa gcgaaggtta ctttcaccac agcgaatact
      361 atgacgggtc agactgacct gctgaaaaac accaaagaaa ctgaaatcgg tggtgccaca
      421 ggcgttggcg tacgtattct ggatagccag agcggcgaag tgactttagg tactccggta
      481 gtaattacat tcaacaatac taacagctat caggaactga acttcaaagc tcgtatggaa
      541 tccccgggca aagatgctac cccgggcaac gtttacgctc aggctgacta taagattgct
      601 tacgagtaat cacattaggc cgggttcgcc cggccacatc attaacggat taatgataag
      661 tggaacagat gtataaaaaa ttaaaattaa ccacaataag cgaattgatt aaaaatattt
      721 attgttcatt atccgttctc atcattggtt gtgcgtcagc ttatgccgtt gaattcaaca
      781 aagatttaat cgaagccgaa gatcgtgaaa acgttaacct ttcccaattt gaaactgatg
      841 gccaattacc cgtaggtaaa tactcattaa acgctcttat taataataaa aggacgccaa
      901 tccatctgga cctgcaatgg gtattaattg ataaccaaac tgcagtttgt ctgactccag
      961 agcaattaac attattaggc tttactgatg agattattga agaggctcag caaaacctga
     1021 tcgatggttg ttaccctatc gaaaaagaaa aacaaattac aacttatctc gataaaggga
     1081 aaatgcaatt atccatatct gcacctcagg catggttaaa atacaaagat gcaaactgga
     1141 cgcctcctga actttgggat catggtattg ctggggcatt tcttgactac aatttatatg
     1201 cctctcatta tgcaccacat cagggcgata attcacaaaa tataagttcc tatgggcagg
     1261 ctggggttaa tcttggggcc tggcgcctgc gtactgatta ccagtacgat cagtcattta
     1321 acaatggcaa aagccaggcg aacaacctgg attttccgcg tatttatttg tttcgcccaa
     1381 tcccagcaat taatgcaaaa ctaactatag gtcaatacga tactgaatcc tctattttcg
     1441 actctttcca tttttctggc gtttcgttga aaagcgatga gaatatgttg ccgcctgatc
     1501 tacgtggtta cgcaccgcaa atcacgggtg ttgcacaaac gaatgcaaag gtcactgtct
     1561 cacagaacaa ccgtattatt tatcaagaaa atgttcctcc tggcccattt gctattacta
     1621 atttattcaa tacattacag gggcaactgg acgtcaaggt tgaagaagag gacgggcaag
     1681 tcacgcaatg gcaagttgca tctaatagta ttccttatct gacgcgtaaa gggcagattc
     1741 gctacaccac tgctatgggt aaaccgacca gcgttggtgg tgattcctta caacaaccct
     1801 tcttctggac tggtgaattc tcatggggtt ggctgaacaa tgtatccctg tatggtggtt
     1861 cagttttaac aaaccgtgat tatcaatctc tggctgccgg cgttggcttt aaccttaact
     1921 cattgggttc attatctttt gatgtcacac gatctgatgc tcagttgcat aatcaggata
     1981 aagaaacggg ttatagctac cgcgctaact attcaaaacg ttttgaatct accggtagcc
     2041 agctcacttt cgctggttac cgtttctctg ataaaaactt tgtgacaatg aatgaatata
     2101 tcaatgacac taaccattac atgaattatc agaatgaaaa agagagttat attgtcacgt
     2161 ttaaccagta tcttgaatca ttaaggttaa atacatacgt aa
//
LOCUS       DAZPLI010000305         2188 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZPLI010000305 DAZPLI010000000
VERSION     DAZPLI010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.308"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            20..307
                     /locus_tag="ACHUU3_004378"
     CDS             20..307
                     /locus_tag="ACHUU3_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084252.1"
                     /translation="MSTRNIHVNTASYTILVAGREKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            426..1247
                     /locus_tag="ACHUU3_004379"
     CDS             426..1247
                     /locus_tag="ACHUU3_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR2084253.1"
                     /translation="MRLASRFDRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(1542..2144)
                     /locus_tag="ACHUU3_004380"
     CDS             complement(1542..2144)
                     /locus_tag="ACHUU3_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFR2084254.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICQATDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQ
                     RRLAYASEVYRIYTGIKSSKGIRIPVTKKSLPEINSVQNN"
BASE COUNT          594 a          467 c          517 g          610 t
ORIGIN      
        1 agagagagga gaaagctgga tgagtacccg aaatatccac gttaacaccg cgtcgtacac
       61 gattcttgtt gccgggaggg aaaaaaacac cggggaagag tgggacgtcc tggaattcag
      121 cagcctgact gagctgaaaa aatatcgcaa aagccacccg gaaaagatgg ccttcagcta
      181 tagctacgcg ctcagccggg gtgtggatac gcagttccgc catatcaaca ttgcggaagc
      241 cgatcacttc aaacagttcc tgcgtcagat taagcgtgcc ggcctcgata tccgggcgat
      301 ctgctgaccg gatgtgatga atatccctcc tgattgttgc aatcttgtta atagagatcg
      361 ggaggaatga tattaagcgg aaaaattttt tattaaattt attctttcat aaaggagtag
      421 ctgttatgcg attagcttcc cgttttgatc ggtataattc catccgtcgt gaacgtcctt
      481 taacggatga tgaattaatg cagttcgtgc cttcggtatt ttccggtgat aaacatgagt
      541 cccggagtga acgttatacg tatattccaa caatcaatat catcaataag ttacgtgatg
      601 aaggtttcca gccattcttt gcctgtcaga gtcgggttcg tgatttggga cgccgcgaat
      661 acagtaaaca tatgttacgt cttcgcaggg aagggcatat taacgggcag gaagttcctg
      721 aaattatcct gcttaattca catgatggtt catccagtta tcagatgatc cccggaattt
      781 ttcgttttgt ctgcacaaat ggcctggtgt gcggaaataa ttttggcgaa atccgtgttc
      841 cacataaagg tgatattgtc gggcaggtta tcgagggagc gtatgaagtg ctcggtgtct
      901 ttgataaggt cactgataat atggaggcga tgaaagaaat tcatcttaac agtgacgagc
      961 aacatttatt tggcagagct gcactgatgg tcaggtatga agatgaaaat aaaacgccag
     1021 tgacgcctga acaaataatt actccccgtc gtcgggaaga taaacagaac gatctctgga
     1081 caacctggca gcgggttcag gagaatatga taaaaggtgg attatcgggg cgaagtgcct
     1141 ccgggaaaaa taccaggaca agagccatta caggtattga tggtgatata agaatcaaca
     1201 aggcgttatg ggtgattgcc gaacagttca gaaagtggaa gtcatgataa tataagtaaa
     1261 gccccgaaaa tttttcgggg ctttacttat attatcattc ttcggcatcg ctgtgtcgtc
     1321 tgatatacct caattccatc aaccggcttt ccggttgtct gtaaatattg tgcccgcgaa
     1381 aaatggtctg gcaggaggct gcgcagttac cgggtatata taagatggat tcccgcttcg
     1441 cggggcttcg gccagtcagc attgtcactc atatgatatt tttgtgtgtg gacttcatgc
     1501 cgcttttgcg gcataaccgg tatctgacaa tgtctgtaag attaattgtt ctgcacgctg
     1561 ttaatttccg gaagtgattt cttcgtgacc ggaatccgga tacctttact gctttttatc
     1621 ccggtataaa tccggtaaac ctctgatgcg taggcaagac gtctctggtt ctggcgttcg
     1681 gtcttcctga atccggcatt gtatgcacca acggcctccc aggagacgcc ccatttttta
     1741 aaggctattg ccagataata agcaccggta taaatgttca tgcagggatc tgttgtcaga
     1801 tgttccggct taattccata gcgggccagt tcgttaaaat gctgggaatc tacctgcatc
     1861 agtccgctgc catatcccgt taccggatta ataccgatgg cattaacccg gtaacgggat
     1921 tctttccatg atattgctct cagtaaatca ggatctattt tgtaatcccg gcctgcaaga
     1981 tcaaagcaat cagtggcctg gcagatctca tttataaaca tcaggcagat ggctaacatc
     2041 cattttttca ttttcccacc tctggtgact ttatccgtaa ataatttaac ccactccaca
     2101 aaaaggctca acaggttggt ggttctcacc accaaaagca ccacacacta cgcaaaaaca
     2161 agtttttgct gatttttctt tataaata
//
LOCUS       DAZPLI010000306         2172 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZPLI010000306 DAZPLI010000000
VERSION     DAZPLI010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.309"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            90..1586
                     /gene="ravA"
                     /locus_tag="ACHUU3_004381"
     CDS             90..1586
                     /gene="ravA"
                     /locus_tag="ACHUU3_004381"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR2084255.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
     gene            1580..>2172
                     /locus_tag="ACHUU3_004382"
     CDS             1580..>2172
                     /locus_tag="ACHUU3_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084256.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQ"
BASE COUNT          557 a          540 c          550 g          525 t
ORIGIN      
        1 cccccctttg agactatttt agccaccagc aagaaacgtc tatactcgca atttacgcag
       61 aacttttgac gaaaggacgc cacttcatta tggctcaccc tcatttatta gcggaaagaa
      121 tttcccgcct gagcagttcg ttggaaaagg ggctttatga acgtagccac gccatccgct
      181 tgtgtttatt agcggcatta agtggtgaaa gtgtgttcct ccttggcccg ccaggtattg
      241 ccaaaagttt gatcgcccgg cgcttaaaat tcgcctttca gaatgcccgc gcgtttgaat
      301 atctgatgac ccgcttctcc acgccggaag aagtttttgg tcccctttct attcaggcgc
      361 taaaagatga agggcgctat gaacgtttaa ccagcggtta cctgccggaa gcagaaattg
      421 tctttctgga tgagatctgg aaagcgggcc cggcaattct taataccttg ctcaccgcca
      481 ttaacgagcg ccagttccgc aacggtgcac acgtagaaaa aatcccgatg cgtctgctgg
      541 tggcggcctc caacgagctg ccggaagcag acagcagtct ggaagcgtta tatgaccgca
      601 tgctgattcg tctgtggtta gataaagtgc aggataaagc gaatttccgc tccatgctga
      661 ccagtcaaca ggatgaaaac gacaatccgg ttcctgatgc cttgcaggtc acagatgaag
      721 aatatgaacg ctggcagaaa gagattggtg aaattacgct gcccgatcat gtatttgagc
      781 tgatttttat gctgcgccag caactggata aattaccgga tgcgccttat gtctcggatc
      841 gtcgctggaa aaaagcgatc cgattattgc aggccagcgc cttttttagc ggtcgcagtg
      901 ctgttgcccc ggtagatctc attttgctga aagattgcct gtggtatgac gcgcaaagcc
      961 tgaatttgat acaacaacaa attgatgtat tgatgaccgg tcatgcctgg caacagcaag
     1021 ggatgttgac tcgccttggc gcgattgtgc aacgtcacct gcaactccag cagcaacaaa
     1081 gcgataaaac agccttaacg gtaattcgtc tgggcggcat tttcagccgt cgtcagcagt
     1141 atcaacttcc tgttaacgtt actgcttcca ctctgactct actgctgcaa aaaccgttaa
     1201 aactgcatga tatggaagtg gttcatatct cctttgagcg tagcgcgctg gaacagtggc
     1261 tgagcaaagg tggtgaaatt cgcggtaaac tgaacggtat cggctttgcc cagaaactga
     1321 atctggaagt tgatagcgcc caacatcttg tcgttcgcga tgtaagttta caaggcagta
     1381 cgctggcact tcccggttca tcggctgaag gtctgcctgg tgaaataaaa caacaactgg
     1441 aagagcttga aagcgactgg cgcaagcaac acgctttatt cagcgaacag caaaaatgtc
     1501 tgtttatccc tggcgactgg ttaggtcgca ttgaagccag cctacaggat gtcggtgcac
     1561 agattcacca ggcacaacaa tgctaacgct ggatacgctt aatgtgatgc tggccgtcag
     1621 cgaagaggga ttgatcgaag agatgatcat cgcactgctg gcctcaccgc agctggcggt
     1681 cttctttgaa aaattcccac gcctgaaggc ggcaatcact gatgatgttc cccgctggcg
     1741 tgaggcgctg cgcagtcggc tgaaagatgc ccgagtcccg ccggaactca ccgaagaggt
     1801 gatgtgctat cagcaaagcc agctcctctc cacgccgcag tttattgtgc agctaccaca
     1861 gatcctggac ttactgcatc gtctgaattc cccatgggca gaacaagccc ggcagttggt
     1921 tgatgctaac agcacaataa cttcagcgtt acacacgctt tttctccagc gttggcgttt
     1981 aagtctgatc gtgcaagcaa cgacgttaaa tcaacagcta ttagaagaag aacgcgaaca
     2041 actgttgagt gaagttcaag aacgcatgac gctgagcgga caacttgaac cgattctcgc
     2101 agataacaat actgcagctg gtcgtctgtg ggatatgagc gccggtcagc ttaaacgtgg
     2161 cgactatcag tt
//
LOCUS       DAZPLI010000307         2168 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZPLI010000307 DAZPLI010000000
VERSION     DAZPLI010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.310"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(468..1019)
                     /locus_tag="ACHUU3_004383"
     CDS             complement(468..1019)
                     /locus_tag="ACHUU3_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR2084257.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFTDEAVSGSVK
                     ASLRNGFSRLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            1818..2114
                     /locus_tag="ACHUU3_004384"
     CDS             1818..2114
                     /locus_tag="ACHUU3_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR2084258.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          628 a          466 c          422 g          652 t
ORIGIN      
        1 aggcttactt agtggaaaga gaggggatat gtgaccatag gggaacccct cctgttaaaa
       61 gactacttag aagtctgagg ttttagtaaa atctgtgaat atggcttctc agacgctctg
      121 taacgcattc taagcgattt taccccttta ttttaaggtg gttagtacat ccctataggt
      181 tacccctaaa aatggggtta aaactcgcta cagagctttc tatgagatta gcttctaatc
      241 gaatcaacta tgaggtttgg taggattgtg caaaatctgt acagtaactt ttcagaatct
      301 gaaaattttt ataaaaaatg actagccctc tttgaatctc atcttccccg aatcttcaaa
      361 aatatctgac agatgtctgt agctgctaag tacccgtcac accagctatg tgaccccctt
      421 ggcaaaaaag ccaaatatca gctacatcca tgtaactatt cagtaactta tttagcaagt
      481 ctgtaataag ttgctctaga gattcctaac tcttcctgaa tctgcaacct cgtcttacct
      541 tgtgaaataa gcgtctgtac agcctctgaa gaagctttta caggcctccc catatgtacc
      601 ccttcagctt tagcacgttc aataccagcc tttctacgct ctgctatcaa gcttctttca
      661 agctgtgcca acgttgacat aatgcctaac attgcttcac ccatagcgct tgataagtca
      721 aacccttctc taaggctgat aactgtcaca cctttagcct gtagcgcttt aaccgttgat
      781 aacacatcaa ttgtgtcacg tccaagacgg tctacagcta ccacaactac ggtgtcacct
      841 tctcgtgcat aagctaatag tctgctgaaa ccgtttctta aagaagcttt gacagaacca
      901 ctgacagctt catcagtgaa ccatttagca atgttgtacc ctgcctcttc aatttgcatt
      961 tgctggttag tgatggattg gtcatctgta ctcaccctgc aatatccaag tatcgccata
     1021 atgttcccct tagaagtctc aaaagttagt ctcataagtt tagctgtctc ataatccaaa
     1081 aacaaccttt tgagactact tgagggtgca ttctaccgtt acgttgaaag tgtctcaaaa
     1141 ggaagtcctt ttaagactgg ttatgaagga gctatgaggt tgattagggg ttggtacaag
     1201 cccaactaac cgaatcaact atgaagattt taggagggta actgtaacca tcactttcta
     1261 actttcacgt aggaaattgt taagtggttt gatagttaca ccatgtgacc aagcatctat
     1321 atatcgttat atcgccctgt atatagcact atatgcagtg tcgagagagg aacgactaac
     1381 acgatacagt ttaccctgaa aagagacttc acagattatt tatatatttc aataagttag
     1441 ggtaaaaagg gactataccg agactgtata gaatctgtgt agaattgcat agatacttgg
     1501 aaatctacta ttaaattgca tagggactca cctgatattg tcctgttttt aacaggtctc
     1561 ctagaaaacc gaatagactt cattcaaggg tcattgaagt cataatcatt tcagtagaaa
     1621 aaccaagtct tgattttaaa tgcaaatagc aatcatattc aataaggcaa gacgatcagg
     1681 atcagtgaaa atgtcatttt tggcgtaatt gaccttactt tatgcatggt cttaacaaag
     1741 acatcatctt tccgttgcag atcttcgccc tttcaaataa cgtactttac aactttcccg
     1801 aacaaggagt tgtgcccgtg ttgtatgtta tttacgctca agacaaagct gatagcctcg
     1861 aaaaacgcct ttccgttcgt ccggcacatt tagcacgttt acagttactg catgatgaag
     1921 gtcgtttgtt gacagctggt ccaatgccag cggtagacag taacgatcca ggggctgcgg
     1981 gatttactgg ctcaaccgtt attgctgaat ttgaatcgct ggaagcagct caggcctggg
     2041 ccgatgctga cccgtacgta gcggcaggtg tctatgaaca cgtttcagtg aaaccattta
     2101 agaaagtgtt ctgaatagag attcaatatt taagggcacg gtttttgcaa ccgtgttctt
     2161 gtttggcc
//
LOCUS       DAZPLI010000308         2159 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZPLI010000308 DAZPLI010000000
VERSION     DAZPLI010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.311"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            270..725
                     /locus_tag="ACHUU3_004385"
     CDS             270..725
                     /locus_tag="ACHUU3_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HFR2084259.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            725..895
                     /locus_tag="ACHUU3_004386"
     CDS             725..895
                     /locus_tag="ACHUU3_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFR2084260.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            888..1178
                     /locus_tag="ACHUU3_004387"
     CDS             888..1178
                     /locus_tag="ACHUU3_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR2084261.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            1175..1537
                     /gene="rusA"
                     /locus_tag="ACHUU3_004388"
     CDS             1175..1537
                     /gene="rusA"
                     /locus_tag="ACHUU3_004388"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFR2084262.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1534..1674
                     /locus_tag="ACHUU3_004389"
     CDS             1534..1674
                     /locus_tag="ACHUU3_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFR2084263.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            1760..2143
                     /gene="quuD"
                     /locus_tag="ACHUU3_004390"
     CDS             1760..2143
                     /gene="quuD"
                     /locus_tag="ACHUU3_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFR2084264.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
BASE COUNT          562 a          495 c          567 g          535 t
ORIGIN      
        1 gtcattggtt agatccatag ttctggctgt gattaggtga aagtatacaa ggtttttaaa
       61 gcgaagatca ctgttttggc ttcaagatag ttttgattta tcgtaatcaa tccgccataa
      121 tcctctcatc ggagcctgaa caactccggt gacttctgcg catttaaggg gacttaaatg
      181 cgaccacaat ctgaactcat caccttgtca cagatgcaga aatgcacctg cgattttctg
      241 cattctgcgg tttccgttag ggaggccgta tgactctgcc agtagacggc atcaaactcc
      301 atcgcggtaa cttcgcggcc atcggccagc agattcagcc attgctggat gccgggcaat
      361 gcttacgcct gcaggttaag ccgtggcgcg agaagcgcag cctgtcgcag aacgcgctca
      421 gccacatgtg gtacacggag atcagcgaat atctcatcgc ccgcggcaag accttcgcta
      481 cgcctgagtg ggtcaaagac gcgatgaagc acacctatct cggctacgaa agcaaagacc
      541 gtgtagatgt cgtgtccggc gaggtcacca ccgtccaatc cctccgccat acgtcagagc
      601 tggaaaccgg cgagatgtac atcttcctgt gcaaagtcga agcctgggcg atgaatatcg
      661 gctgccacct gacaattccg cagagctgtg aataccagca gctgcgcgat aagcaggagg
      721 cgtaatggct acaccgctta ttcgtgtcat gaacgggcac atctacagag tatcaaatcg
      781 tcgtaagcgt aagcctgagc tgaagccatc cgaaatacca acactgctcg gatataccgc
      841 tagcctggtt gataaaaaat ggttgcgact ggcagcaagg aggaatcatg gctgatttga
      901 gaaaagcagc gcgtggtcgg gaatgccagg taagaatccc tggcgtatgt aatggcaatc
      961 ctgaaacgtc agtactggca catatccggc tggctggatt gtgcggtacc ggtatcaaac
     1021 cgccagacct gattgccacc attgcatgtt ctgcctgtca cgacgaaatc gaccgccgca
     1081 catattttgt cgatgctgag tatgcaaaag aatgcgcgct ggaaggtatg gcgagaacgc
     1141 aggttatctg gctgaaagag ggggttatta aggcgtgaat acctacagca tcacattacc
     1201 ctggcctccg agcaataacc gctattaccg ccataatcgc gggcgcacac acatcagcgc
     1261 agaagggcag gcataccgcg ataacgtcac ccgaatcatt aaaaacgcaa tgctggatat
     1321 cggcctggct atgccagtga aaatccgtat tgagtgccac atgccggatc gccgtcgccg
     1381 tgacctggat aatctgcaaa aagccgcttt tgacgcactc accaaagcag gtttctggct
     1441 ggatgatgct caggtcgttg attaccgcgt tgtgaagatg cctgttacca aaggtgggag
     1501 gctggaactg accatcaccg aaatggggaa tgaatgatgt ttgagtttta tatggcagaa
     1561 cttcttcgcc accgctgggg gcatctgcgc ttatatcgtt tccccggttc tgttttgacc
     1621 gattaccgaa tactgaagaa ttacgccaaa accctgacag gagcaggagt atgaagtcag
     1681 agataacaat caactaatac tgttttgttg atttttgctt gtaattggcg ttctggtctg
     1741 atttttgtgg agtaagttga tgcgtgatat tcagatggtt cttgagcgtt ggggagcgtg
     1801 ggcggctaat aatcatgaag atgtgacctg gtcgtccatt gccgccggtt ttaagggatt
     1861 aattacttca aaagtaaaat ctcgcccgca atgttgtgac gatgacgcga tgattatttg
     1921 cgggtgcatg gcccgtctga aaaagaacaa cagcgatttg cacgatttat tagtagatta
     1981 ttatgtagtc ggtatgacat tcatgtcact ggcaggtaag cattgctgct ctgatggtta
     2041 tatcgggaaa aggttacaga aggctgaggg cataattgaa gggatgttaa tggcattaga
     2101 tatccggtta gagatggata tcgttgttaa taactctaat taatatgcca attgtttac
//
LOCUS       DAZPLI010000309         2138 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZPLI010000309 DAZPLI010000000
VERSION     DAZPLI010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.312"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(1..800)
                     /locus_tag="ACHUU3_004391"
                     /pseudo
     CDS             complement(1..800)
                     /locus_tag="ACHUU3_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490573.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            complement(802..984)
                     /locus_tag="ACHUU3_004392"
                     /pseudo
     CDS             complement(802..984)
                     /locus_tag="ACHUU3_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
     gene            complement(1006..1317)
                     /gene="traL"
                     /locus_tag="ACHUU3_004393"
     CDS             complement(1006..1317)
                     /gene="traL"
                     /locus_tag="ACHUU3_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFR2084265.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(1332..1682)
                     /gene="traA"
                     /locus_tag="ACHUU3_004394"
                     /pseudo
     CDS             complement(1332..1682)
                     /gene="traA"
                     /locus_tag="ACHUU3_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            complement(1728..>2138)
                     /locus_tag="ACHUU3_004395"
     CDS             complement(1728..>2138)
                     /locus_tag="ACHUU3_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR2084266.1"
                     /translation="VGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLA
                     YTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVD
                     WYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA"
BASE COUNT          529 a          596 c          503 g          510 t
ORIGIN      
        1 gcaccggtat gacgacgggc ggtacggtaa cctttctcaa taaactcacc gaccttgtga
       61 tttttgaagt ccagcagacg ccagccttca tcaatgacgt tcagtttctt gaggttacgc
      121 ggcgtgcggt acatcctgtt ctcgatgtag ataatcaggg agaacatcac cgcaaccagc
      181 agtgacggac ggtcttccag tccgcccagc tccagcacca ccatttttgc gtcatcccgc
      241 agggacggct catcagagtt aaaatactgg ccgtaagtgc cgttggcagt gtactggtca
      301 agcagcacaa tcatttcgtc cagacggctg cggatagtcg gtgacccggc atactgctcg
      361 ctgtcactgg cgtttttcag gaaatccacc acgtcatcaa tacgtgccct gttctctttg
      421 gccagccagg aggccctgac cgcctgcagc agcagacctt catgcacttc atccaggtta
      481 ccgttggggc tggccatcac tgacaactgg tcacggacac gctccgctga ctggtcgata
      541 tcggtgatgt tcgcaaacgg gttaaaacgc agggtttcac cgtccagata tacgcccccc
      601 atgttctcac acagggactt gtagccatcc cccatgtcga acaccacggc aaagcccccg
      661 gagtccagca cgctgcggat aagtggctgt atcagcccgg ttttaccggc cccggaggtg
      721 ccacagaccg ccatgttgta gttggtgtta ttcattcccc ggaagaaaat atcgataaac
      781 gccagctggt tacggtaggt gtgacacggc aaagggcaca ttaaatccca ttggggtcac
      841 ggcagtgcgt tgctcattct gaagacggta gttattcacc ccctgaatga ggttaacaga
      901 gaggctgaga acaataagca ctgacataaa tataaaggcg atggccatta cacgactggt
      961 acttaaacgg gcaccgtgtt ccatataaca atcctggtat cagttctatt taatccactg
     1021 ccggaaacac gaatcgggaa cattatgaaa aataccgcgc agcagggctg ttggcatata
     1081 ccagtaaatc aggtcacgta accaggaact gccccgccct tttttcagtt ttttaatccc
     1141 gaaataaacc agaaccgctg caccaatacc gaacagatat ttcgatgttg tgatacccca
     1201 gccaacacag attgctgcgg ggatcagttc atccagtggc aggccaaacc agcggctctg
     1261 gttggtcagt gtttccggga aacgatattt cttaagttta ttctcgtctc ccaacatcgt
     1321 tttatttcct gtcagaatcc ggcaacagac atacctactg caataaatac agagagtatg
     1381 gcaaagccga ccaggaactt gacgtttttg gtcatcatgt acatgactgc accgaccaga
     1441 acttcagcca gaacaaccca tttaacgata ctggagtcct taccgaaggt cgccttaacc
     1501 gtttcctgac ctttagccat caggtccgtg cctttaccag cagccatagc taactcaggg
     1561 aacaacatca tcagaacagc aaccgggatc actgcacgaa ccaggcgaag catattcaga
     1621 cgagtgaatt tagaaaaaaa cgatttcttt ttgacgggcg cagaagtacc ctgaacactt
     1681 aacgctgaac cgccccgggt ttcctggaga gtgttttatc tgtgaactca ggctgccaga
     1741 tcatcgtttc cgatggaagc ataataagct ttttctgctt ctgccggagg agtatggccc
     1801 agcctttcca gcaatcgtcg attgttatac cagtccaccc acgtgagtgt ggccagttcc
     1861 acttctgcac ggtttttcca gctcttacgg tgtattacct ccgctttgta aagaccattg
     1921 atgctctccg ccatcgcgtt gtcatacgag tcgcctgtac ttcctgttga tgccagtaat
     1981 ccggcttcct taagccgctg tgtgtaggcc agcgatacat actgagaacc tttatcactg
     2041 tgatggaccg tgccggacgg tcgacgggcc cataacgcct gctccagtgc atccagcacg
     2101 aatgtcgtct ccatggacga tgagacccgc caccccac
//
LOCUS       DAZPLI010000310         2131 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZPLI010000310 DAZPLI010000000
VERSION     DAZPLI010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.313"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..1094)
                     /locus_tag="ACHUU3_004396"
     CDS             complement(<1..1094)
                     /locus_tag="ACHUU3_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR2084267.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSN"
     gene            complement(1131..>2131)
                     /gene="abgB"
                     /locus_tag="ACHUU3_004397"
     CDS             complement(1131..>2131)
                     /gene="abgB"
                     /locus_tag="ACHUU3_004397"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFR2084268.1"
                     /translation="SGKTFMVREGVFDDVDAALTWHPEAFAGMFNTRTLANIQASWRF
                     KGIAAHAANSPHLGRSALDAVTLMTTGTNFLNEHIIEKARVHYAITNSGGISPNVVQA
                     QAEVLYLIRAPEMTDVQHIYDRVAKIAEGAALMTETTVECRFDKACSSYLPNRTLENA
                     MYHALSHFGTPEWNSEELAFAKQIQATLTPNDRQNSLNNIAATGGENGKAFALRHRET
                     VLANEVAPYAATDNVLAASTDVGDVSWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSI
                     AHKGMLLAAKTMAATTVNLFIDSGLLQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
BASE COUNT          539 a          536 c          546 g          510 t
ORIGIN      
        1 atgttgctcc agttaaacat ggcgacaaac tgggcgaggg gaaaaaccat cacgataaat
       61 cctgccatct ctttcatcgg ttcaatcatt aactgcggta aatccgcctg acgtcgaatt
      121 gtgcgggtag cgatgccata agccagcgag acaacaaaga aaaaaagaat gatcagtggc
      181 acgatacctt taataaaggg tgatggcatc acggtgtgat taatcggatc gcgcaatatc
      241 ccgttttccg ggatcaccat cagcgcaatc gcagcaataa aaagtagcga tacgacacct
      301 gctatgcgta aaccaaaacg ctgactttcg gtcaatgtct gcagtttctc atcgctgttt
      361 ccctgccatt gacctaaccg tggctcgatg attttgtcgg ttatcaggcc gccaacaatc
      421 gtcagtacga ctacggagct ggccataaaa taccagttat caattacact gacgtgcatt
      481 tgcggattga acgcagccgc cgcttccgtg ctgatccccg acagcaacac gtcggttgtg
      541 acaatcagta aattagccgt aaagccgcaa cctacgcctg caatggcagc cagtaaacct
      601 gcaactggat gcctgcccac cgccagaaaa atcagcgcac ccatcggtgg catgatcact
      661 aacgtcgcat cggaagaaat gtggctgaaa aaagcaataa acagcaccat ataactggcg
      721 tagcgggcat taacatgcga tgccatttta accattagcg ctggcagtaa gccgacgcgc
      781 tccgccagac cggcacctaa aaccagcgcc aggatcgcac caagtggagc aaaaccgcta
      841 aagtttttaa taacattggg taaagaccag tgtaatcctt ccacactgag caggtttttc
      901 accaccaccg gcgttccatc ggtcgggttt ttcgcactga cgccaaaggc cgacaaaatt
      961 gccgtcgtca ccatgagtac gataatcaaa tagataaaga gcaaaaaagg atggagaacc
     1021 ttgttaccaa ttctttcgac ccagccatag agcttcccgg attgggagga cgacggtatg
     1081 gatgacatac tcatgggcat tcctcggttg ttgtgtttgc gttgttgttg ttattttaaa
     1141 ggtgacggtg tcacgttttt cgggataggg cagtgatacg gttgcgtgtc cgttacttgc
     1201 tgatgctctt gttggcattc ttgcaatagc cctgaatcaa tgaagagatt gactgtggtt
     1261 gctgccatag ttttcgccgc cagcagcatt cctttatgag caatagatgt tcgcccctgg
     1321 ctaaccagtt gccacgtatg tagcggtgta ccgacggcaa aacaggggct gaaacactgg
     1381 gcaacaggca gtttccaact aacgtcgccg acatcagtcg atgccgcaag cacgttatcg
     1441 gtggcggcat atggagcgac ctcattcgcc agtaccgttt cacgatgacg tagtgcaaaa
     1501 gccttgccgt tttcgccacc ggttgcggcg atattattca gactgttttg ccgatcgttg
     1561 ggggtgagcg tagcctgaat ttgtttcgca aaagccagtt cttcggagtt ccattccggg
     1621 gtaccaaaat gggatagggc atggtacatg gcattttcta aggtgcgatt cgggagataa
     1681 ctggaacagg ctttgtcgaa gcggcattca accgtggttt cggtcatcaa tgccgcacct
     1741 tcggcgattt tggcgacccg atcataaata tgctgcacgt cggtcatttc gggggcgcgg
     1801 ataagataaa gcacttctgc ctgcgcctgg accacgttgg gcgagatccc gccgctattt
     1861 gtgatggcat agtgtacgcg cgctttttca ataatatgtt cgttgaggaa gttggtgcca
     1921 gtggtcatca acgttacggc atcaagggcg ctgcgtccca aatgagggga attcgcggca
     1981 tgtgctgcga tccctttaaa gcgccatgat gcctgaatgt ttgccagcgt gcgggtattg
     2041 aacataccgg caaaggcttc cgggtgccag gtgagtgccg catccacatc atcaaatacc
     2101 ccctcgcgga ccatgaacgt tttacccgag c
//
LOCUS       DAZPLI010000311         2094 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZPLI010000311 DAZPLI010000000
VERSION     DAZPLI010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.314"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(1..617)
                     /gene="yedA"
                     /locus_tag="ACHUU3_004398"
                     /pseudo
     CDS             complement(1..617)
                     /gene="yedA"
                     /locus_tag="ACHUU3_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            790..1707
                     /locus_tag="ACHUU3_004399"
     CDS             790..1707
                     /locus_tag="ACHUU3_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR2084269.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            1786..1968
                     /locus_tag="ACHUU3_004400"
     CDS             1786..1968
                     /locus_tag="ACHUU3_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR2084270.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
BASE COUNT          534 a          483 c          594 g          483 t
ORIGIN      
        1 gcgccgtcag tttttcacct gcaatcatcg acgcgatcat taacaccacg cctgccgcca
       61 gcatctcaat cgcacccgcc atcatcccta caggtaaggt aatgcgcgag ccataaaccg
      121 agccaaacgc ccagctaatc gagccgatta aaatcagaat cgcgccccac ggattgccgc
      181 ttaaatttcc accgctattg agcatgatga ttccggcaag cccaatggcg atacccaccc
      241 attccagttt gcgcgtttta atgccaaaca ggcggctgaa gcaccagggt aaagaggggc
      301 acggttgcaa ccacaacggc ggcgatgccg gaaggaacat tttgatgctc ggcaaccgtc
      361 accatgccat taccgacagc cagcaataac aggccaatca gcgcggcatt gagcagcgga
      421 cgtagcgggg ggagcttgtg tccgcgcagt agcaaaaatg ccatcaataa aataccggct
      481 gccaggaatc gaacgcctgc catcattaac ggaggccagc tttccacgcc aatccgaatg
      541 acaaaatagg ttgagcccca aatgatatac aacgcaaaca gcgcgccaaa aagcggtaac
      601 aactggcgga aacgcataat ccctcacggt ggaaataaaa aggtggttca tagtaaacgt
      661 gaaaatcatt ctgctggcga gagatataat tgcacttgat tgtaaaaaaa atgttgacct
      721 gtgaagcaag tatcagagct gccgtttttg cttcatactt acacccttca acaataaaaa
      781 tgagagggaa tgcttttggc cgggagtagt ttactgacgt tgctcgatga tatcgccaca
      841 ctactggacg atatctccgt gatgggcaaa ctggcggcga agaaaaccgc cggtgtatta
      901 ggggatgact tatcgctcaa tgcgcaacaa gtttcaggcg tgcgggccaa ccgggaactt
      961 cccgtggtct ggggcgtggc gaaaggatcg ctgattaata aagtgattct ggtgccgctg
     1021 gcgctgatca tcagtgcgtt tatcccgtgg gcgattacgc ctctgttgat gattggtggc
     1081 gcgtttctct gctttgaagg agtagagaaa gtgctgcata tgctggaggc gcgtaaacat
     1141 aaagaagatc cggcgcagag ccagcagcgt ctggagaagc tggcggcgca ggatccgctg
     1201 aagtttgaaa aggacaaaat aaaaggggcg attcgtaccg attttatatt gtctgccgaa
     1261 atcgtcgcca tcacgctggg gattgtggcc gaagcgccgt tgcttaatca ggtgctggtg
     1321 ctttcaggca tcgcgctggt agtgaccgtg ggcgtctacg gtctggtagg ggttatcgtt
     1381 aagattgatg acctggggta ttggctggcg gaaaaatcca gcgcgctgat gcaggcatta
     1441 ggtaaagggt tattgatcat tgcgccctgg ttgatgaagg cgttatcgat tgtcggcacg
     1501 ctggcgatgt tcctcgtcgg cggcggtatt gtggtacatg gtattgcgcc gctgcatcac
     1561 gccattgaac atttcgccgg gcagcaaagt gcagtggtgg cgatgatatt accgactgtt
     1621 ttaaatctga ttcttggatt tatcatcggc ggcatcgtgg tgctgggagt gaaagccgta
     1681 gcgaaaatgc gcggtcaggc acattaatac tcgtacaggc tattatgcaa aattcgccat
     1741 cttcaactaa ggtgaacaag tttcactcga aaaaaggagg cgagtatgag ctttatggtt
     1801 agtgaggaag ttacggttaa agagggcggc ccgcggatga ttgtcactgg atactccagc
     1861 ggtatggttg agtgtcgttg gtatgacggt tacggggtca agcgggaagc ttttcatgaa
     1921 accgagcttg ttccggggga ggggagtcgt tctgcggaag aagtttgaaa gggtatcatt
     1981 aacaccaggc cggatcactt ccggcctggt gtgccaatgc ctgcaatgaa tgatagtcgc
     2041 gtcttatcat gccaccatta taaaattatc gctgttttgc ggtacgcggt gatg
//
LOCUS       DAZPLI010000312         2093 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZPLI010000312 DAZPLI010000000
VERSION     DAZPLI010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.315"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..123)
                     /locus_tag="ACHUU3_004401"
     CDS             complement(<1..123)
                     /locus_tag="ACHUU3_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFR2084271.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPY"
     gene            384..719
                     /locus_tag="ACHUU3_004402"
     CDS             384..719
                     /locus_tag="ACHUU3_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR2084272.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1000..1131)
                     /locus_tag="ACHUU3_004403"
     CDS             complement(1000..1131)
                     /locus_tag="ACHUU3_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFR2084273.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1631..1707)
                     /locus_tag="ACHUU3_004404"
     tRNA            complement(1631..1707)
                     /locus_tag="ACHUU3_004404"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1671..1673),aa:Arg,seq:tct)
     gene            complement(1808..1883)
                     /locus_tag="ACHUU3_004405"
     tRNA            complement(1808..1883)
                     /locus_tag="ACHUU3_004405"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1848..1850),aa:Ile,seq:cat)
     gene            complement(1986..>2093)
                     /locus_tag="ACHUU3_004406"
     CDS             complement(1986..>2093)
                     /locus_tag="ACHUU3_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR2084274.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          677 a          522 c          370 g          524 t
ORIGIN      
        1 ataaggggtt atggccaccg gactaccaaa cggctgccag ccctctttca gtttatgtgt
       61 cagcttttcc gcaagatctg acggcggcgc cgccctgaca acatcatagt gtttaaatgc
      121 catggttctt tccaccatct gaaaaataat tctttaaaat acctgacatg taatacagaa
      181 aaaacacaaa accatacctt aaataaaaac ctgattatca agcagatatg catggataaa
      241 ctacaagacg agatataaac caccctgcat ttaaataaac aataaacaac atcagaaaaa
      301 taattctgct ctatggttta cattcaaaaa tatcatttat acttttcaga acatcaccag
      361 caaggcataa acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat
      421 catgcggaat atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat
      481 cggatgcttt cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca
      541 ataacaaagt gctacctgta accatttaca acgtagtacc attcaataaa acattctgga
      601 attcaatcaa aaatagccag gaatgcccta caaatacaga taacgtattg aatgaatgct
      661 ttaataaccg ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag
      721 gagtttactc acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag
      781 tggcgctatg tgccattgca tggtgccggg tgcctcccgg tgaattcagt accagcacct
      841 gaatccgcga ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac
      901 catgccagtt tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg
      961 tgaagtattt aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt
     1021 tttcagcaac gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccagcac
     1081 tgacattatc cggctgctgc aatgccattc acaaaaacag taagcaatca ctttttaccg
     1141 taacaggtga taatccagat atgtatctac cccagatgag taatccgaag ttcatccata
     1201 ccacaggtcc tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca
     1261 accatgaact catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt
     1321 cccgaaagac gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt
     1381 gctgcattga ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata
     1441 ttcactctac ccacaccata tgaatgataa atatttaatg ctgaaaaaac cattagaccg
     1501 tataacaaac actcaatcaa tacttaacag aacttttatt tttgacaaac ataaaatatt
     1561 ttcaacaata tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga
     1621 agaatgaata tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat
     1681 tgctctgtcc ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc
     1741 gcccccgcac caacaaccct gttatcatgt cgtctgctct tcctgataag ctaatggcgg
     1801 tttgtgatgg tggcccttgc tggatttgaa ccagcgacct ggcgattatg agtcgctcgc
     1861 tctcaccact gagctaaagg gccgggagca gaataataac ggtccgtaat taattccgca
     1921 ataaaaaacc cgctcggcgg cgggttgtag aaactcttct aacgtcaggc ataaaacgcc
     1981 catagttatg acgaatttac cacagattcc ggaaaaatca acctagttac cttttttaac
     2041 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ata
//
LOCUS       DAZPLI010000313         2088 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZPLI010000313 DAZPLI010000000
VERSION     DAZPLI010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.316"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            81..1253
                     /gene="istA"
                     /locus_tag="ACHUU3_004407"
     CDS             81..1253
                     /gene="istA"
                     /locus_tag="ACHUU3_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HFR2084275.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..2050
                     /gene="istB"
                     /locus_tag="ACHUU3_004408"
     CDS             1253..2050
                     /gene="istB"
                     /locus_tag="ACHUU3_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HFR2084276.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
BASE COUNT          552 a          505 c          590 g          441 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg tgatcctgct
     1561 gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga aagcggtgga
     1621 tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac tgatgaaagc
     1681 gaaacaggaa aaccggctgg agcgtcagct acagcaactg agttatgccc gggtgttgat
     1741 cctggatgaa ataggctatc tgccgatgaa cagagaggaa gccagtctgt tcttccggct
     1801 actgaaccgt cgatatgaaa aagcgagcat catactgacg tcaaacaaag ggttcgcaga
     1861 ctggggagaa atgttcggag atcacgtact ggcaacagcg atcctggatc ggctgctaca
     1921 tcactcaacc acgctgaata tcaaaggaga gagttaccgg ttaaaagaga aacgtaaagc
     1981 tggagtgctg accaaaaaca caacgccaat cagtgatgat gaaatggtgg aaagcggaca
     2041 gcatcagtaa cgaaagtatt tagcgggcat gaaaatggca aataacgg
//
LOCUS       DAZPLI010000314         2051 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZPLI010000314 DAZPLI010000000
VERSION     DAZPLI010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.317"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..314
                     /locus_tag="ACHUU3_004409"
     CDS             <1..314
                     /locus_tag="ACHUU3_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084277.1"
                     /translation="ALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYS
                     GAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQR
                     A"
     gene            416..1870
                     /gene="amn"
                     /locus_tag="ACHUU3_004410"
     CDS             416..1870
                     /gene="amn"
                     /locus_tag="ACHUU3_004410"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR2084278.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIDIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          503 a          505 c          480 g          563 t
ORIGIN      
        1 tggcgcttga agcacaatct attttctgga tagttttctt ctccataatg ctggcaaaca
       61 ttgcgcatga catggtggtg tgtgtgcaac aaccgatgtt taccgaaatg tttggtgcca
      121 gttatcgcta tagtggcgct ggagtcggtt atcaggttgc cagtgtggtt ggcggtggat
      181 ttacaccttt tattgccgct gcactcatca cttactttgc cgggaactgg catagcgtcg
      241 ccatttattt gctggctgga tgcctgattt ccgcaatgac cgctttgttg atgaaagaca
      301 gtcaacgcgc ttgatagcct ggcgaagatc atccgatctt cgccttacac ttttgtttca
      361 catttctgtg acatactatc ggatgtgcgg taattgtatg gaacaggaga cacacatgaa
      421 taataagggc tccggtctga ccccagctca ggcactggat aaactcgacg cgctgtatga
      481 gcaatctgta gtcgcattac gcaacgccat tggcaactat attacaagtg gcgaattacc
      541 tgatgaaaac gcccgcaaac aaggtctttt tgtctatcca tcactgaccg taacctggga
      601 cggtagcaca accaatcccc ccaaaacgcg cgcatttggt cgttttactc acgcaggcag
      661 ctacaccacc acgattactc gccctactct ctttcgttcg tatcttaatg aacaacttac
      721 gttgctgtat caggattatg gtgcgcatat ctctgtgcaa ccctcgcagc atgaaatccc
      781 ttatccttat gtcatcgatg gctctgaatt gacacttgat cgctcaatga gcgctgggtt
      841 aactcgctac ttcccgacaa cagaactggc gcaaattggc gatgaaactg cagacggcat
      901 ttatcatcca acggaattct ccccgctatc gcattttgat gcgcgccgcg tcgatttttc
      961 cctcgcacgg ttgcgccatt ataccggtac gccagttgaa cattttcagc cgttcgtctt
     1021 gtttaccaac tacacacgtt atgtggatga attcgttcgt tggggatgca gccagatcct
     1081 cgatcctgat agtccttaca ttgccctttc ttgtgctggc gggaactgga tcaccgccga
     1141 aaccgaagcg ccagaagaag ccatttccga ccttgcatgg aaaaaacacc agatgccagc
     1201 atggaattta attaccgccg atgctcaggg tattactctg gtgaatattg gcgtgggacc
     1261 atcaaatgct aaaaccatct gcgatcatct ggcagtgcta cgcccggatg tctggttgat
     1321 gattggtcac tgtggcggat tacgtgaaag tcaggccatt ggcgattatg tacttgcaca
     1381 cgcttattta cgcgatgacc acgttcttga tgcggttctg ccgcccgata ttcctattcc
     1441 gagcattgct gaagtgcaac gtgcgcttta tgacgccacc aagctggtga gcggcaggcc
     1501 cggtgaggaa gtcaaacagc ggctacgtac tggtactgtg gtaaccacag atgacaggaa
     1561 ctgggaatta cgttactcag cttctgcact tcgttttaac ttaagccggg ccgtagcaat
     1621 tgatatggaa agtgcaacca ttgccgcgca aggatatcgt ttccgcgtgc catacgggac
     1681 actactgtgt gtttcagata aaccattaca tggtgagatt aaacttcccg gtcaggctaa
     1741 ccgtttttat gaaggcgcta tttccgaaca tctgcaaatt gacattcggg cgatcgattt
     1801 gctgcgcgca gaaggcgacc gactgcattc gcgtaaatta cgaaccttta atgagccgcc
     1861 gttccgataa taagttctga accttcttat cagaaataga tgtgaaggag taagtaagac
     1921 acctggcaaa tagcctgcaa taatcgtggg ctgtttgctt ccttgggcgg atacgagttt
     1981 tattatcgtc ttaatgattt ccacatatta aaagcaagta tgctttcaaa acacaattat
     2041 aaaaaatccc g
//
LOCUS       DAZPLI010000315         2046 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZPLI010000315 DAZPLI010000000
VERSION     DAZPLI010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.318"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..633)
                     /locus_tag="ACHUU3_004411"
     CDS             complement(<1..633)
                     /locus_tag="ACHUU3_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HFR2084279.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLPRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ASCHDCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYAC"
     gene            791..1900
                     /locus_tag="ACHUU3_004412"
     CDS             791..1900
                     /locus_tag="ACHUU3_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFR2084280.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRAEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPSKPKKEKSSSREGDYMEGAEGNVF
                     VLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLAHGRAELYPEMHGTVTGFKSEIDN
                     QDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
BASE COUNT          534 a          559 c          532 g          421 t
ORIGIN      
        1 gcacgcatag gcaggcgcgg ggaacgtttc gtttttgcca cttttcgggt tgtaggcgat
       61 gaagtccggc cataagagca tcacctcacg ttcgttgaat ttctggcggt aggtaattgc
      121 ctcagccatc gtgttacagt catgacatga ggcatacaca aacgcgcgca gtttacccgc
      181 aatcacgcac agggattttg tcaccgcctc cgtgtccagc tccggcgcgg ccagaatacg
      241 cggacggtat ccgatgcttt catcctgctc tgcaacaagc agcgcataca tccccgtata
      301 gctgccgtca gattcagaac caccgataac cagttgatcc tgcgtttttc cgtcttcttc
      361 tttgtgttca gccacgcgaa cgacgatcac ctttgtgctc acctggtctg cgatggcctt
      421 aagcgcacga taaagcgtcc ccgttgttcc gcattttccc agcacgtcat tgacgcgggg
      481 cagcagtgtg ggcttgttca gcgggaacag ctccgcgtcc gcatcatccg ccgttgccac
      541 gataccgata acgctggaat caacatcgtt aatcgctgtt accaggtcgg tattttccgt
      601 aacacgggca ccatgaaaac gagtttcact catagcttca accccttgta tccgttaaat
      661 gattcggcaa caatcatcac ccaccacgcg cgtaatctca cccctgcgcc attctcccgc
      721 cacggcgaca acaaaaagca gtaaccccct ccgcacgcac atgcgaccat gccacacagg
      781 gagggagcag atgaccgaca ccaccatgca attgctcagt cagggcacag accccgtgaa
      841 aatgccggat tttgatattc tcgcggaggg taaaacgctg tcaggcgtgg cagagcgcct
      901 gatgagcctg tcactgaccg acaaccgggg atttgaggcg gaccagctca ccatcacgct
      961 ggatgatgcg gatggtcagt tgcagctacc gccacggggc gcgcgcctga cggttctcat
     1021 tggctggaag ggagaaccgc tgacagaaaa aggcacttac attgttgatg aaatcgctca
     1081 cgaaggaccg ccggacaggc tgactgtttc agccagaagc gcagattttc gggctgaatt
     1141 taacgttaaa cgtgaggtgt cctggcatga tgtgaccgtt gagcgtgtgg tatccgccat
     1201 cgctcatcgg tatggtctga aaccgcaaat cagcgaaatg ctgatggata tcgaaatcga
     1261 ccacgccgac cagaccgaag aaagcgacat gtccttcctt acgcgcatgg cggaaatgct
     1321 gggcgcaatc accacggtaa aaagcggtaa tctgttattc atcatgcccg gtggtggcgt
     1381 gaacgcacag ggccagccgt tgccctcgtt cgccatcaca cgcagcagcg gcgatcgcca
     1441 tcagttccgc attgctgacc gcgaagcgta tacgggggta cgcgcttact ggcttgatct
     1501 taattacggg aaaaagaaaa aagtcagcgt gaaacgccgc aaaccgtcaa aaccaaaaaa
     1561 ggagaaaagc agcagccggg aaggcgacta tatggaaggt gcggaaggca acgtgtttgt
     1621 gttacgcaaa acttatcaga acgagcaggc agcaagacgt gcagcggcgg caaagtggca
     1681 gcagctacaa cgcggagccg catcattctc catcacactg gcgcatggac gtgcagaact
     1741 ctaccccgaa atgcatggca cggtaacagg atttaaaagc gagattgata atcaggactg
     1801 gattattgca aaagccgagc acaccattga taacagcggc tttaccacgc agcttgagct
     1861 tgaggcaaaa atcccggaat ggatagcaga aacagagtga gcaacttaaa tgtattaact
     1921 cagaacggat caacacactc acaacataga gtcaatatag ctgctcgctt tgttccagca
     1981 aggatgccta tttctaagga aaaacagagc agaaacatat ggtgttttcc tacctgacat
     2041 cagggg
//
LOCUS       DAZPLI010000316         2014 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZPLI010000316 DAZPLI010000000
VERSION     DAZPLI010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.319"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(136..606)
                     /gene="argR"
                     /locus_tag="ACHUU3_004413"
     CDS             complement(136..606)
                     /gene="argR"
                     /locus_tag="ACHUU3_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR2084281.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            1041..1979
                     /gene="mdh"
                     /locus_tag="ACHUU3_004414"
     CDS             1041..1979
                     /gene="mdh"
                     /locus_tag="ACHUU3_004414"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR2084282.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT          522 a          438 c          519 g          535 t
ORIGIN      
        1 ttaccgtatg tattcattgt gtgaatgata tgtcgcggta aaacgcacta ttcgttaaat
       61 taatcgcagg ggaaagcagg gggttgagag gaataagcaa cattttcccc gccgccagaa
      121 gcgacggggc agagattaaa gctcctggtc gaacagctct aaaatcgctt cgtacaggtc
      181 tttgacggtg aaaccgttag caggggtggt aaagatggtg tcatcgccag cgatggtgcc
      241 cagaatacct tctgctttgc ccagtgagtc cagcaggcga gcaattaact gcgccgcgcc
      301 agggctggta tgaatcacga caactgcatc gttgtagtcg atatccagca ccagattctt
      361 caatggactg gaggtggttg gtacacccag ttcagctggc aggcagtaaa ccatttccat
      421 tttggcattg cgtgtacgta cagcaccaaa cttggtcaac atccgcgaga ctttagactg
      481 attaatattg tcaaagcctt gctcctgcaa cgcggcgacg atttcgccct gggagctaaa
      541 tttctcttct ttaagtaatg ctttaaatgc tttaactagt tcttcttgct tagccgagct
      601 tcgcataagt cacccgatat ggtggttgat acaacattat tgtgcataca gatgaatttt
      661 tatgcaaaca gtcagccctg aagaaggctg aaataatgtt atgaaagagc gggattttat
      721 caaatttcgt tattgagaaa catgcctgcg tcacggcatg caaattctgt ttaaaagtaa
      781 attaattgtt atcaaattga tgttgttttg gctgaacggt agggtatatt gtcaccacct
      841 gttggaatgt tgcgctaatg cataagcgac tgttaattac gtaagttagg ttcctgatta
      901 cggcaattaa atgcataaac gctaaacttg cgtgactaca cattcttgag atgtggtcat
      961 tgtaaacggc aattttgtgg attaaggtcg cggcagcgga gcaacatatc ttagtttatc
     1021 aatataataa ggagtttagg atgaaagtcg cagtcctcgg cgctgctggc ggtattggcc
     1081 aggcgcttgc actactgtta aaaacccaac tgccttcagg ttcagaactc tctctgtatg
     1141 atatcgctcc agtgactccc ggtgtggctg tcgatctgag ccatatccct actgctgtga
     1201 aaatcaaagg tttttctggt gaagatgcga ctccggcgct ggaaggcgca gatgtcgttc
     1261 ttatctctgc aggtgtagcg cgtaaaccgg gtatggatcg ttccgacctg tttaacgtta
     1321 acgccggcat cgtgaaaaac ctggtacagc aagttgcgaa aacctgcccg aaagcgtgca
     1381 ttggtattat cactaacccg gttaacacca cagttgcgat tgctgctgaa gtgctgaaaa
     1441 aagccggtgt ttatgacaaa aacaaactgt tcggcgttac cacgctggac atcattcgtt
     1501 ccaacacttt tgttgcggaa ctgaaaggca aacagccagg cgaagttgaa gtgccggtta
     1561 ttggcggtca ctctggtgtt accattctgc cgctgctgtc acaggttcct ggcgttagtt
     1621 ttaccgagca ggaagtggct gatctgacca aacgcatcca gaacgcgggt actgaagtgg
     1681 ttgaagcgaa agccggtggc gggtctgcaa ccctgtctat gggccaggca gctgcacgtt
     1741 ttggtctgtc tctggttcgt gcactgcagg gcgaacaagg cgttgtcgaa tgtgcctacg
     1801 ttgaaggcga tggtcagtac gcccgtttct tctctcaacc gctgctgctg ggtaaaaacg
     1861 gcgtggaaga gcgtaaatct atcggtaccc tgagcgcatt tgaacagaac gcgctggaag
     1921 gtatgctgga tacactcaag aaagatatcg ccttgggcga agagttcgtt aataagtaat
     1981 tgattagcga ataataaaaa accggagcac agac
//
LOCUS       DAZPLI010000317         2005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZPLI010000317 DAZPLI010000000
VERSION     DAZPLI010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.320"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..389
                     /locus_tag="ACHUU3_004415"
     CDS             <1..389
                     /locus_tag="ACHUU3_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084283.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHUU3_004416"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHUU3_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR2084284.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="ACHUU3_004417"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="ACHUU3_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR2084285.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="ACHUU3_004418"
     tRNA            complement(1806..1881)
                     /locus_tag="ACHUU3_004418"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="ACHUU3_004419"
     tRNA            complement(1890..1966)
                     /locus_tag="ACHUU3_004419"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          529 a          484 c          487 g          505 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggctt
//
LOCUS       DAZPLI010000318         1906 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZPLI010000318 DAZPLI010000000
VERSION     DAZPLI010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.321"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..101
                     /gene="mgtT"
                     /locus_tag="ACHUU3_004420"
     CDS             <1..101
                     /gene="mgtT"
                     /locus_tag="ACHUU3_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFR2084286.1"
                     /translation="GDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(216..1106)
                     /gene="dgcZ"
                     /locus_tag="ACHUU3_004421"
     CDS             complement(216..1106)
                     /gene="dgcZ"
                     /locus_tag="ACHUU3_004421"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFR2084287.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1361..1753)
                     /gene="ydeI"
                     /locus_tag="ACHUU3_004422"
     CDS             complement(1361..1753)
                     /gene="ydeI"
                     /locus_tag="ACHUU3_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFR2084288.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT          551 a          447 c          370 g          538 t
ORIGIN      
        1 acggagataa tccctcacct aaccggcccc ttgttacagt tgtgtacaag gggcctgatt
       61 tttatgacgg cgaaaaaaaa ccgccagtaa accggcggtg aatgcttgca tggatagatt
      121 tgtgttttgc ttttacgcta acaggcattt tcctgcactg ataacggatc gttgacacag
      181 tagcatcagt tttctcaatg aatgttaaac ggagcttaaa ctcggttaat cacattttgt
      241 tcgtcaataa acatgcagcg atttcttccg gtttgcttac cctcatacat tgcccggtct
      301 gctcttccaa tgaccacatc cagaggctct tcaggaaatg cgcgactcac acctgctgtc
      361 acggtaatgt tgatatgccc ttcagaatgt gtgatggcat ggttatcgac taactggcaa
      421 attctgacac ctgcacgaca tgcttcttca tcattagccg ctttaacaat aatgataaat
      481 tcttcgcccc cgtagcgata aaccgtttcg taatcacgcg tccaactggc taagtaagtt
      541 gccagggtgc gtaatactac atcgccgatt aaatgcccgt aggtatcatt aaccaattta
      601 aatcggtcaa tatccaacaa cattaaataa agattcagag gatcagtgtt gcgtaactga
      661 tgatcaaagg attcatcaag aacccgacgc cccggcaatc ccgtcaaaac atccatattg
      721 ctacggatcg tcagcaaata aattttgtaa tcggttaatg ccgcagtaaa agaaagcaac
      781 ccctcctgaa aggcgtcgaa atgcgcgtcc tgccagtgat tttcaacaat agccagcatt
      841 aattcccgac cacagttatg catatgttga tgggcagaat ccattagccg aacgtaaggt
      901 aattcatcgt tatcgagtgg ccccagatga tcaatccacc gaccaaactg gcacagtcca
      961 taagaatggt tatccgttat ttctggctta ctggcatctc tcgcgaccac gctgtgaaac
     1021 atactcacca gccactggta gtgggcatcg atagccttat tgagatttaa caagatggca
     1081 tcaatttccg ttgtcttctt gatcattgcc actccttttt cacagttcct tgtgcgcgct
     1141 attctaacga gagaaaagca aaattacgtc aatattttca tagaaatccg aagttatgag
     1201 tcatctctga gataacattg tgatttaaaa caaaatcagc ggataaaaaa gtgtttaatt
     1261 ctgtaaatca cctctgcatt atcgtaaata aaaggatgac aaatagcata acccaatacc
     1321 ctaatggccc agtagttcag gccatcaggc taatttattt ttatttctgc aaatgagtga
     1381 cccgaacgac ggccggcgcg cttttcttat ccagactgcc gctaatgttg atcatctggt
     1441 ccggctgaac ttctcgtcca tcaaagacgg ccgcaggaat aacaacatta atttcaccgc
     1501 tcttatcgcg aaaaacgtaa cggtcctctc ctttgtgaga aatcaaatta ccgcgtagtg
     1561 aaaccgaagc gccatcgtgc atgttttttg cgaaatcaac ggtcattttt tttgcatcat
     1621 cggttccgcg atagccatct tctattgcat gaggcggcgg tggcgctgca tcctgtttta
     1681 aaccgccctg gtcatctgcc aacgcataag gcatgacaag aaaacttgct aatacaatgg
     1741 actgaaattt catactaact ccttaattgc gtttggtttg acttattaag tctggttgct
     1801 atttttataa ttgccaaata agaatactgc caattgttat aaggcattta aaatcagccc
     1861 actagctgtc aaatattcag agaatttgac tcactaaagt taagaa
//
LOCUS       DAZPLI010000319         1870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZPLI010000319 DAZPLI010000000
VERSION     DAZPLI010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.322"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            186..689
                     /locus_tag="ACHUU3_004423"
     CDS             186..689
                     /locus_tag="ACHUU3_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR2084289.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(768..1019)
                     /gene="yecJ"
                     /locus_tag="ACHUU3_004424"
     CDS             complement(768..1019)
                     /gene="yecJ"
                     /locus_tag="ACHUU3_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR2084290.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1134..1220)
                     /gene="azuC"
                     /locus_tag="ACHUU3_004425"
     CDS             complement(1134..1220)
                     /gene="azuC"
                     /locus_tag="ACHUU3_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR2084291.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1483..1806
                     /locus_tag="ACHUU3_004426"
     CDS             1483..1806
                     /locus_tag="ACHUU3_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR2084292.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          486 a          439 c          412 g          533 t
ORIGIN      
        1 tataaacttg atgatttaag cattttctta tacccgttca gacgttattc ttatttcaga
       61 tcatcgtcag aattgactcc acgatcacat ttcggaccgg cagaaaggaa ttattctgca
      121 aacagtaatt atggtgtttt gatttatctt gcacctctcc acttctggat ataaggatat
      181 taggtatggc aaccgctgga atgcttctca aactcaactc tcaaatgaac cgcgagtttt
      241 acgcatccaa tctctacctt cacctgagta actggtgttc tgaacagagt ctgaacggca
      301 ccgccacttt ccttcgcgcc caggcacaga gtaatgtgac ccaaatgatg cgcatgttta
      361 actttatgaa gagtgtcggc gctaccccca tcgttaaagc cattgatgtt cccggtgaaa
      421 aactgaactc tctggaagaa ctgttccaaa aaacgatgga agaatacgag caacgttcta
      481 gtacgttggc acagttagcc gatgaagcga aagaactgaa tgatgattca accgtcaatt
      541 tcctgcgcga tctggaaaaa gaacagcagc atgatggtct gttgctgcaa accattcttg
      601 atgaagtgcg cagtgcgaaa cttgcgggta tgtgccctgt gcagaccgac caacatgttc
      661 tgaatgtcgt gtcacaccag ctgcattgat catcatcggc gctaatgcat tgcgccgatg
      721 aaggttttga gaaaccgctg cctcatctgt ttgaagcagc ggttttttta atgggattca
      781 ccctgtgggg taaacttgag ttcaataagc gcgatggctt tttggattgc ccgcatggtg
      841 accgggtctg cggcggcggg atggttagta aagtcgatat tcttcagctg actggacatt
      901 ttttcacgaa cttcaacggg cgcgattaca tcgagaacat ccagaatttg tttgataacc
      961 aactggcaag caaccacatc agaaaccagt tcctgatcgg cattcagcgg ctgggacatc
     1021 gtaaactcct gatagcattt tgaaagccgt tatagtagcg gcttcacatc ttcagcgata
     1081 gtcacatcca ccgtcatcag gacacaaaaa aacctgccgg agcaggtttt ttgttatcgg
     1141 aacatattgc ctggcggtac gtctttgaac gtcttgcaat agttattgaa catacttttc
     1201 aggattttgc gcagtttcat cgcggcactc cgaccatttg ttatacaggt gttattgtct
     1261 ttgcgcttat aatatgacaa ccatcacaaa aatcaatctt tatgtgatac aaatcacata
     1321 aatacccctt taatgttata aaaatgataa tcaaaaaaca gcccccctat ttctgacacc
     1381 tacagatggc aagaaatagc gcctgccagg cgtcttttcc ggccattgtc gcagcgctgt
     1441 aacgcgtaaa atagtgcttt ctcttactct tctggctgga ccatgagact tctgattctg
     1501 actctttcat taataacgct ggcggggtgt acggttactc gccaagccca tgtgagcgaa
     1561 gttgacgcgg caacaggtat tgtacggttg gtttatgacc aggctttttt gcagcatgcc
     1621 cataccgatc gttatgtcag tcgcgggatc gccgatcgcg catgccagca ggaaggctat
     1681 acccacgcgg tcccctttgg tcagccagta ggcaactgta gcctttttgc gggttcctta
     1741 tgtttgaata cggaattcac tttgtcatat cagtgccatc attccgcctt ccctgtcttt
     1801 ctctaaatat tttaatcagc gaggggatct tcgctgatta aagaaatagc ggtaatgcgt
     1861 tttattccca
//
LOCUS       DAZPLI010000320         1803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZPLI010000320 DAZPLI010000000
VERSION     DAZPLI010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.323"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            76..618
                     /locus_tag="ACHUU3_004427"
     CDS             76..618
                     /locus_tag="ACHUU3_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2084293.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            698..1372
                     /locus_tag="ACHUU3_004428"
     CDS             698..1372
                     /locus_tag="ACHUU3_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR2084294.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            1388..>1803
                     /locus_tag="ACHUU3_004429"
     CDS             1388..>1803
                     /locus_tag="ACHUU3_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR2084295.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
BASE COUNT          571 a          321 c          397 g          514 t
ORIGIN      
        1 ttttgatgtg agaaatttgt ttccaagaga tacagactct taacaaccaa aaaaaatttt
       61 attaaggaac atattatgaa acgttcaatt attgctgccg ctgtcttttc ttcttttttt
      121 atgagcgctg gagtatttgc tgcagacgtt gataccggaa cattaactat taaggggaat
      181 attgcagaat ctccgtgtaa attcgaagcg ggtggtgatt cagtaagtat taatatgccg
      241 actgtaccaa ccactgtctt tgaaggtaaa gctaaatatt ctacctatga tgatgcagtc
      301 ggtgtaacca gcagcatgtt aaaaattagc tgcccgaaag aagttgctgg tgtaaaactc
      361 tcgttgatta ccaacgataa aataaccggt aacgataagg cgatagccag tagcaacgat
      421 accgtgggtt actatctcta tttaggtgat aacagcgatg tcctggatgt ttctgcacct
      481 tttaacattg agagttataa aacagcggaa ggtcaatatg ctattccgtt taaagcaaaa
      541 tacctgaaac tgacagataa ctcagtgcaa tcaggtgatg tgttatcttc tctggttatg
      601 cgtgtggcgc aggattaata aataaaattg taagtttctc caggtaataa acatggccgc
      661 tatcccatgg cggcctttta atttaagagg cattaaaatg aaaggattat tatctttact
      721 cattttttct atggtccttc ctgcacatgc cggaattgtt atctacggga cgcgcattat
      781 ttacccggca gaaaataaag aagtgatggt gcagttgatg aaccagggaa accgttcttc
      841 gctgctgcag gcgtggattg atgatggcga tacgtcatta ccaccagaaa aaattcaggt
      901 tcctttcatg ttaacgccac cagtggcaaa aataggggca aattccgggc agcaagtaaa
      961 aatcaaaatt atgccgaata aactgcccac taataaagaa agcatttttt atctgaatgt
     1021 tctggatatt ccaccaaata gtccagagca agaaggtaag aatgcactga agtttgcgat
     1081 gcaaaacaga attaagttgt tttaccgacc agcgggtatt gctccggtaa ataaagcgac
     1141 atttaaaaaa ttgctggtaa atcgcagtgg caatggtttg gtgataaaaa atgactcagc
     1201 taattgggtg acgatttcgg atgtcaaagc taataatgtc aaagtcaatt atgaaactat
     1261 tatgattgcc cccttagaaa gtcagagtgt taatgtcaaa agtaataatg caaataactg
     1321 gcatctgacc attatcgatg accatggcaa ctatattagt gacaaaattt aattgcagga
     1381 gctgcctatg ttgagaatga ccccacttgc atcagcaatc gtagcgttat tgctcggcat
     1441 tgaagcttat gcagctgaag aaacctttga tacccatttt atgataggtg gaatgaaaga
     1501 ccagcaggtt gcaaatattc gtcttgatga taatcaaccc ttaccggggc agtatgacat
     1561 cgatatttat gtcaataagc aatggcgcgg gaaatatgag attattgtta aagacaaccc
     1621 gcaagaaaca tgtttatcaa gagaagttat caagcggtta ggcattaata gcgataactt
     1681 cgccagcggt aagcaatgtt taacatttga gcaacttgtt cagggtggga gctatacctg
     1741 ggatatcggg gtttttcgtc tcgatttcag tgtcccgcag gcctgggtgg aagaactgga
     1801 aag
//
LOCUS       DAZPLI010000321         1727 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZPLI010000321 DAZPLI010000000
VERSION     DAZPLI010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.324"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..355)
                     /locus_tag="ACHUU3_004430"
     CDS             complement(<1..355)
                     /locus_tag="ACHUU3_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR2084296.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLA"
     gene            complement(355..849)
                     /locus_tag="ACHUU3_004431"
     CDS             complement(355..849)
                     /locus_tag="ACHUU3_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFR2084297.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            1207..1383
                     /locus_tag="ACHUU3_004432"
     CDS             1207..1383
                     /locus_tag="ACHUU3_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084298.1"
                     /translation="MRDKPANHKRGKNPVCIVFDYSRTLAQKEFPVGLRSWLIREYGD
                     DTAHDVSGLNTFIR"
     gene            complement(1525..1677)
                     /locus_tag="ACHUU3_004433"
     CDS             complement(1525..1677)
                     /locus_tag="ACHUU3_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084299.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESRLNISLLMLDIEHFA
                     RGKGLR"
     gene            complement(1665..>1727)
                     /locus_tag="ACHUU3_004434"
     CDS             complement(1665..>1727)
                     /locus_tag="ACHUU3_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR2084300.1"
                     /translation="IRDQTALRTLQEYIRTQCLR"
BASE COUNT          441 a          475 c          399 g          412 t
ORIGIN      
        1 tggcgagtcg ttttttggag tgttcgcggg ctttttcctc cgtcatctga ataatcagca
       61 tatcagcagg atcgcaaatc acgttgtaaa tcacccagcc gtcaatcagg ccgatagtct
      121 tgccggttcg tgccgggcca acaaatatca ctgcgtcgta ttcacgcgag gccaggcagt
      181 tcatcggctc aataacatac ggtgccacca gcggatccca cgggactgag ttccctgccc
      241 ccatgggcac ccgcatatac tgagcaacgg catcagcaac ccgcattcgt ctcggtgcgc
      301 gaaggatata acctgaatcg gttcgtgctg cctttgcggt ttcctgattc agcattactc
      361 ctcctgctgt aattcctcct catcatccgc acctgcttca gtcacccgca gggctatctg
      421 atcgcgcaga tcatcaataa tggactgaac acggctcaca gcggcaggct gcagaccgca
      481 gtcacgttca agaatatccg gtaatgtctc cagcacctgc acgaccgctt ttgcccagat
      541 ggcaaactcc cgtctgacat cactggccgg aatgagttgt gccgtttcct gttcgaactt
      601 aagacgctca cgttcagact gataccaggc tttgcgctca tgcgcgtcca tttcgccttc
      661 tgcaaccggc ggtggtaatg ccagaaatgc cgacacaata tcaaccaccc gataaagctt
      721 gaggttgctt tcatgccccc ctgcaacggg tagattttgc agccttgccg cagcagtctg
      781 gcgatgtaca cctgacagtg ccgccagttg actgatattc agcgtcagat tttttaactc
      841 tcgatccata cccgctccag aatgttttaa acatgcatct tgcgaacaac tttaggcaaa
      901 cggtgttagt gatgaacaaa aaacaatcaa aatcgacacc ataaaaataa aaccactgta
      961 atatcaatcc attacagtag tggtgatgac gaatgaaatt tcaaaaacta gcctttttcc
     1021 gcgccgctcc cgccccgtgg caggccaccc caccgggagg acccgtcagc ctgacagcca
     1081 tgacgaacgt ctgatacagc gccctgcatg aatgacatcg ggataatcca gaaaggaata
     1141 gcatcgtacc cacaagaatc agtgtaagtg tcctgtttct tccacccccg cacaggactg
     1201 gcgagcatga gggacaaacc cgcgaaccat aaacgcggta aaaacccggt gtgcatcgtt
     1261 tttgattatt cccgcacact cgcgcagaag gagttccccg tcgggctacg gtcatggtta
     1321 atacgggaat acggcgacga tacagcgcat gatgtgtcag gcttgaatac ctttatccgt
     1381 taaaagggat atcagttaag ttatcccgtg tagggtataa gccattatca aagccactct
     1441 gtaggaagtg gcttttgtaa tggcaataaa aagccccgcg aatgcgaggc taaatcctgg
     1501 tatttgtaat gactggctct tatctcaacg cagcccctta ccgcgcgcaa aatgctcaat
     1561 atcaagcatc agcaatgaga tgtttaatct ggattcactc cagaagtgag caccaccctg
     1621 tctacagagc cagatgtgaa ggatgatgag taaaattatc gctatcatcg aaggcattgc
     1681 gtcctgatgt attcctgaag cgttctcagt gctgtttggt cgcggat
//
LOCUS       DAZPLI010000322         1704 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZPLI010000322 DAZPLI010000000
VERSION     DAZPLI010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.325"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            448..795
                     /locus_tag="ACHUU3_004435"
     CDS             448..795
                     /locus_tag="ACHUU3_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFR2084301.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(815..1405)
                     /locus_tag="ACHUU3_004436"
     CDS             complement(815..1405)
                     /locus_tag="ACHUU3_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084302.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLQACHDAGMEVRVQINESAAP"
     gene            complement(1402..1662)
                     /locus_tag="ACHUU3_004437"
     CDS             complement(1402..1662)
                     /locus_tag="ACHUU3_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084303.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
BASE COUNT          392 a          471 c          418 g          423 t
ORIGIN      
        1 tttttataat aacagaaaga atatatatca tacttaacgg aagagataat atgaaaacag
       61 tacccgtata aacagcaagc aatcccattt ttccaggtgt atcagcaaaa aaaccgctgt
      121 tctagaaatc aggccgggta aatttcagag ctgtatcttc aatataccat tttgcaaccg
      181 gatacaaaac cattccgcag agagaaatac accagaacag taatctgtat ttaaaatcat
      241 aatcccatga catatacagc atatatcctc ccgtcaccca tccccaccac atattattaa
      301 aataatattt tctgttcatc tgccagtttt cctttctttt gcacttttgt cagtgtactg
      361 atgcatgaca acataaccac agcatgataa taataatcaa taacaataag ctgtgtcacg
      421 tttacatcat ggaacggagg tgctcctgtg acttttgagc agaaaaaagc ccgggccatt
      481 gccctgatgg acagcaaaaa gatgtggcgg agcaattatg cccctcctct tctgcgtatt
      541 ctctggcggc tgggtatccg gcttccgcca ttacccttta tgccgttctg gcaggtcacg
      601 gtgctgacgg ggggcttatg gggcatttcc tggggatgtg caatgtggtt tatctactgg
      661 gggccgtcag gaatggtggc aggtgaggcc attattataa gtatcacggg gggttttttg
      721 tccggactgc tcatggcctc tttccactgg tggcgcagga aagttaaccg actgccacca
      781 tgggatgatg tgtaaaccgt gcacaggcgt gttcttacgg agcggcactt tcatttatct
      841 gtacgcgaac ctccattcct gcatcgtgac aggcctgcag ccactgcgcc acttccagcg
      901 gatcgccctc ccggcgtacc acccagcctt ctttattcca taactgcaga caggtgctgc
      961 cgtcgagacg caccacaaaa tccccacggc aggcctgata cgttatgccg ttcagctcca
     1021 ccgccagatc agtggcagaa gtgccgcctg cgggcagacc tgctgccggg accagcggca
     1081 gtacgacgac ttctgccgca cgctgttctg cccggatgcg gtcctgttct gccagcagca
     1141 gaggctgtgc cacggcccgg ccggcgtccg tcagctccac tgccagctgc aggttcggag
     1201 cgcgcagggt acgcagccag ccggcatcct ccagacgacg gcaggaggcg cgcaggtttg
     1261 gcccgtagac cggggcttcc ccgctttgct ccagcacccg ttctatgtcc cgcgtcagca
     1321 cgggtcctgg ccgtttttca tcaagggcag ccagcacaat cagcactctc cgctgcagtg
     1381 gcgacggtcg gcggctctga gtcatttcca gtcatcctcc ggccagatga gctttctgag
     1441 gcgctgatgg tcggcgtcaa tgatctcctg tctctttatg gcattcattg aggtatagcc
     1501 atttatggcc agttcatgcc agaagatcag cacggctccg gctttggctg actcgcagag
     1561 ataagttacg ccgtggctgt ccagattgca ttcccggatg cgtttttccg caaaccgggt
     1621 cagttgttca tagctgagtg aaaacgtcat ttcctgatcc atcctgcacg ttccatctga
     1681 taatgttctg acaggtaaaa atca
//
LOCUS       DAZPLI010000323         1692 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZPLI010000323 DAZPLI010000000
VERSION     DAZPLI010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.326"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..1100
                     /gene="ompC"
                     /locus_tag="ACHUU3_004438"
     CDS             <1..1100
                     /gene="ompC"
                     /locus_tag="ACHUU3_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR2084304.1"
                     /translation="KLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYFT
                     DDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAGL
                     KISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDFF
                     GLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKSD
                     RTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNKA
                     QSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYVD
                     YKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1264..>1692
                     /locus_tag="ACHUU3_004439"
     CDS             1264..>1692
                     /locus_tag="ACHUU3_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084305.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKESKLENIYIDHNNLESLDL"
BASE COUNT          510 a          346 c          351 g          485 t
ORIGIN      
        1 tgaaattaaa aatagttgcg gtggttgtaa ctggtttgtt agctgcgaac gtagcacacg
       61 ctgccgaagt ctataacaag gatggtaata aactcgacct ttatggcaag gttaccgctc
      121 tacgttattt tactgatgat aagcgtgacg atggtgataa aacttatgcc cgtctcggct
      181 ttaaaggaga aacgcaaatc aatgatcaaa tgattggttt tggtcactgg gaatatgatt
      241 ttaaaggcta taacgatgaa gccaacggct cgcgcggcaa caagacccgt ctggcctatg
      301 ctggtttaaa aattagtgaa tttggctctc tggactatgg tcgtaactac ggtgtcggct
      361 acgacattgg ttcatggacc gatatgttgc cagaatttgg tggcgatacc tggagtcaga
      421 aagatgtctt catgacatac cgtaccaccg gtctagcaac ctatcgcaac tacgatttct
      481 ttggcttaat tgaagggctg aactttgccg cgcaatatca aggcaaaaat gaacgtactg
      541 acaacagtca tctttatggt gctgactaca cgcgtgccaa cggtgacggt ttcggtatct
      601 cctcaactta tgtttatgat ggctttggta tcggtgcggt atataccaaa tccgatcgga
      661 caaatgcgca ggaaagagcc gctgctaatc ctctcaatgc ctccggtaag aatgcagaac
      721 tatgggccac aggtataaag tatgatgcca acaacatcta ctttgcagct aattacgctg
      781 aaacattaaa catgaccacc tatggggatg gttatatctc taacaaagca caaagttttg
      841 aagtggtggc gcaatatcaa ttcgacttcg gcttgcgccc atcactcgct tacctgaaat
      901 cgaaaggcag agatctgggc cgctacggcg accaggacat gattgagtat atcgacgttg
      961 gtgcgacgta tttcttcaac aaaaatatgt cgacctatgt tgattataaa atcaacctga
     1021 ttgatgaaag cgactttacc cgtgccgtag atattcggac cgataacatc gtcgctacgg
     1081 gcattaccta tcagttctaa cgtcctctcc tgaccagccc ttctctgggc tggtttttga
     1141 tttaagtgct gctgtatgac tccttaagta aaaatgagca atttttgata tttctggtca
     1201 ttcacctcaa ttaatattat tccatatcct ccggggacat cttactataa tatttattga
     1261 tgagtgatta tgaccaatat taatacagct tgtgtaaaaa ataatgccag ttatcaactt
     1321 aataacgcat tacctaacaa agaaaccatc tccagcaatt tttgtgaacg actagaacaa
     1381 tggggtaata aatcggttaa taatggtgaa gaaagagcaa ttgccgtgga gcgaattaaa
     1441 gaagcttaca attcgaacat ggcatctctt gatttgtcat atcttgattt gagtgaatta
     1501 ccgcctatcc cctcgacagt taatacgtta aatctggaaa acaattgtct aacttgtctt
     1561 gacttagctg acaatgccag tctcgttaat atcaacctca gcgttaataa aattaacacg
     1621 ataacctttc caaaagaatc aaaactggaa aacatttata ttgatcataa caatctggaa
     1681 agtttagatt ta
//
LOCUS       DAZPLI010000324         1661 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZPLI010000324 DAZPLI010000000
VERSION     DAZPLI010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.327"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(81..191)
                     /locus_tag="ACHUU3_004440"
     CDS             complement(81..191)
                     /locus_tag="ACHUU3_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084306.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            complement(244..648)
                     /locus_tag="ACHUU3_004441"
     CDS             complement(244..648)
                     /locus_tag="ACHUU3_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFR2084307.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(869..1600)
                     /gene="bluR"
                     /locus_tag="ACHUU3_004442"
     CDS             complement(869..1600)
                     /gene="bluR"
                     /locus_tag="ACHUU3_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFR2084308.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          450 a          357 c          350 g          504 t
ORIGIN      
        1 attgaaacgg gcgtatggca cacccgttgt ttttataaat atattaaccg ttataaaata
       61 acgtatagaa agtcaagtga tcacatttca aatatcaatt tgatagtatt ggcctggtga
      121 ttatttatgg gtagcaataa aaggacagta tttatcatcc atagggatag tctctgtact
      181 tttattccca ttatgctaat gccttactga attatgaagc atttcttaag catccaactt
      241 tagctagatt aatggtttat tattttctac atcttcaata tataaaagcg tattatcaat
      301 ggcgtagtaa ctgcgtttgt tatgattaac atcagtaacc caacggaaaa cgcccgcgcc
      361 tgccagtgtt gaacagtatt cccgaaatgt agattttccg taaatatgaa gcaatgcggc
      421 ctcttttatt ttagcagggt tcttggtcgt actaactttt aacaggttcc tggttcctct
      481 taataacaaa accgtgtcat cgtgagtaat aattctgatg ttatccgtag ccagataata
      541 aatgtaatgt gcaatacggt gatgttttaa ttctgaataa aaccaggaga agttttgctc
      601 ttttctcact tgctcaaaca tcttttgaaa aacaacgacc tgatccatca ggataataac
      661 ctcttgttag ttgtgagact gcgtagtgtg cacgatcggt tttaccactt caatctggtc
      721 tgtcctttgg ctgtgatatg tacagagtgt gatagaggga ttacctgaat tcttccggtg
      781 agcattttgc aacggaccag ctccggtaca aatgctgttg tgggttcaga ttataacatt
      841 ctgtctaagg ggcgggataa aggtgaaatt agggggcatg aaagatgact ttataacctt
      901 gctcacccca atgttgtaaa agttcgtttt gccttctcgt tggtgccatg cctgtccaga
      961 caatcaatgt ttgcgtcggg aacagttccg ggcgcggcga ttcaatggga tcagcaagaa
     1021 cagaaatgtg ccatcctgac aatgataaac gccatgcttc cagccacaat cgtgctctct
     1081 cttcaacatt ccacgccatc agaatggctt ctttaccggg cttacggcgc atttcgaaaa
     1141 gtgaggctgc tgcatattca attaatgcgc cgtcaaacat actgctcata atgcgggagg
     1201 tgttgtgatc aagcacgaga cgctggcgaa caggaaggta aacatgatta atcaattgat
     1261 ctactgggta ctctcgaccc agtgaaataa ttctcgcgcg tagtttggca ggattagcca
     1321 tacgaagaat tgacatcatc tcttcttgca ggcggttcca gtcatcttcc gtatcctggc
     1381 tggtggtttc cagtaatgct ttaactttgc ctacagggac gccattactt atccaacgct
     1441 tgatctcttc gatgcgttgt atgtcttctt catcaaagag tcggtgtccg ccttcactgc
     1501 gctgtggttt taacaaaccg tagcggcgtt gccaggcccg gagagtgaca ggattaatcc
     1561 cgcaacgttc agcaacatca ccaatgctgt aataagccac aattcctcct tgcggtcata
     1621 aatctccgcc gcctgtacac gacccaataa tactttgtac a
//
LOCUS       DAZPLI010000325         1658 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZPLI010000325 DAZPLI010000000
VERSION     DAZPLI010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.328"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            59..1312
                     /locus_tag="ACHUU3_004443"
     CDS             59..1312
                     /locus_tag="ACHUU3_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2084309.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(1361..1480)
                     /locus_tag="ACHUU3_004444"
     CDS             complement(1361..1480)
                     /locus_tag="ACHUU3_004444"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR2084310.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
BASE COUNT          340 a          421 c          407 g          490 t
ORIGIN      
        1 cgattttcct aacctatgta taagcctgat ccacaggcat atttagcaag gatttcaagt
       61 gagccgtact acaactgttg atggcgctcc ggcaagcgac actgacaagc aaagcatttc
      121 tcagccaaat caatttatta aacgcggtac gccgcaattt atgcgcgtca ccctggcgct
      181 gttctctgcc ggactggcaa cctttgcact cctctattgt gtgcagccta tccttccggt
      241 gctttcgcag gagtttggct taacccccgc gaacagtagt atttcactgt ccatttccac
      301 ggcgatgttg gctattggtt tgctgtttac tggcccccta tccgatgcca ttggtcgcaa
      361 accagtgatg gtcacggcgc tactgttggc ctccatttgt acgttacttt cgacaatgat
      421 gaccagctgg cacggcattt tgattatgcg cgccttgatt gggctttcgt taagtggcgt
      481 ggcagctgtt ggcatgactt atcttagcga ggaaatccac cccagtttcg tggccttttc
      541 gatggggttg tatatcagcg gcaactcaat tggcggcatg agcggacgct taattagtgg
      601 tgtcttcacg gactttttca actggcgaat tgctctggcg gcaatcggtt gtttcgcgct
      661 agcctcggcg ttgatgttct ggaaaatcct tccggaatca cgccattttc gcccgacttc
      721 gctgcgccct aagacgttat ttatcaactt tcgtctgcac tggcgtgacc ggggattacc
      781 gttattgttc gcagaaggct ttttgctgat ggggtcgttc gtcacgctgt ttaattacat
      841 cggctatcgg ttgatgcttt ccccctggca tgtcagtcag gccgtggttg gcttattatc
      901 gctggcttat ttgaccggta catggagctc acccaaagcc ggaaccatga ccacccgcta
      961 cgggcgcggt ccggtgatgt tgttttcgac gggggttatg ctgtttggtt tactgatgac
     1021 cttattcagc tcgctgtggc tgatctttgc cggaatgtta ctcttctcag caggattctt
     1081 cgcagcccac tcagttgcca gcagctggat tggcccccgc gcaaaacgcg ctaaaggcca
     1141 ggcctcctcg ctgtatctgt tcagttacta tctggggtcg agtattgccg ggacgctggg
     1201 tggtgttttc tggcataact atggctggaa cggcgtcggc gcatttattg ctctgatgct
     1261 ggtcattgct ctgctggtcg ggacgcgttt gcatcgtcgt ctgcacgcct gaaaaataag
     1321 cccggactgc ggtaactaca cgtccggact tattgcttgc tcaaaccaac gttaatagcc
     1381 atcctaaaat agacgaagcg ccagccaatt cccgcagcgc gtctagcgtc atcaggatta
     1441 taagtaccca aataaacgga ttcattttgc tgtgtgtcat ttattactga tgcgcagtta
     1501 ttctaccgct ttgtaagtag taaaatagtt aacccgatca agactactat tattggtagc
     1561 taaatttccc ttaagtcaca atacgttatt atcaatgctg taatttattc agcgtttgta
     1621 catatcgtta cacgctgaaa ccaaccactg gaagtctg
//
LOCUS       DAZPLI010000326         1657 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZPLI010000326 DAZPLI010000000
VERSION     DAZPLI010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.329"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            211..>1657
                     /gene="istA"
                     /locus_tag="ACHUU3_004445"
     CDS             211..>1657
                     /gene="istA"
                     /locus_tag="ACHUU3_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HFR2084311.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRA
                     LNARLRELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPD
                     YHVEYARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHM
                     PERHRRHGTWTPERLIEQGNRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKYG
                     PERLEKACYVALHYNAPDRRFIDNLLRHHRDN"
BASE COUNT          419 a          463 c          414 g          361 t
ORIGIN      
        1 atcgcagttt ggcattcgaa tcgatcatga tttggcatcc atctccgatc acagtttggc
       61 atcacaatcg atcacgattt ggcatacttc cgatcattga ttggaattcg ccagtcactc
      121 cgggaattaa ctcttttcac cacagtctcc attgccgtgt ttaaaccagt ggagacgtca
      181 atgtccaaaa agagaaaatc caggagcact atggaaactt gttttaagat ccttcagctc
      241 aagttcgata agaagctgac taaccgttgt atcggactga cgttacacat cagcgcctca
      301 accgtttttg aggttcttgc ccgcttcaaa gcctcttctc tgtcatggcc attacctgcg
      361 gatatatccc atgacacgct ggagaaactc attttcccgc ccaaagacac ctctgcgtca
      421 gagcttgtta tgcctgacat gctgtacttc gatacggaaa tgagaaaacc tggcgtgacg
      481 cgccaactgt tgtggatgga atacaaggca caggcgggtg acaaagcgat gggatactca
      541 cacttctgcc gttgctaccg gaagtggaaa aaaacgcgac gcctctccat gcgacaggag
      601 catcgtgctg gcgaaaagct ctttatcgac ttctgtggtc ctacggtccc tgttattaat
      661 ccggacaccg gagagatacg ccgcgtggca atcttcgtgg ctgtaatggg ggcgtccaac
      721 tatacctatg ttgaagcgtg cgaaggtcag gacatgatgt catggttaaa tgcccacagc
      781 cgttgcctga ccttcctggg cggcgtaccg aagctgctca tccccgataa cctcagaagc
      841 gcagtgaaaa aagccgatcg ctatgagcct gtcatcaacg acagttatca ggcgctggct
      901 gagcactatg gcacggttat tatcccggcc cgcccgcgca aacctaaaga taaaccaaaa
      961 gcagaaaacg gcgtcctcat cgtcgagcga tggcttcttg cccgcatccg taatgaaacc
     1021 ttccacacgc tcagggcgct gaatgcacgc cttcgggagt tgctgacgga catgaataac
     1081 cgaccaatga agggatacgg taatcaaacc cgcgcagaac gcttccgcat gctggatgca
     1141 ccagcactct ctccgctgcc tctggagcct tatgaataca cggaatacaa agccgtgaag
     1201 gttggccctg actaccacgt tgaatatgcc cgtcactggt actccgttcc acacgaactg
     1261 gtcggtcagc gtctgtcact gaaggtgggg cagtcggtgg tacagctctg gcacaagggg
     1321 cagtgtgtgg cgcaacatcc gcgcagcaca catgaatata aacacacaac caatccgctt
     1381 cacatgccag aacgccaccg gcggcatggg acatggacac cggaacgcct tatcgagcag
     1441 ggaaaccgta caggtccgtc cacaggccgg gtggttgaat ccatgctgaa agccaaaccc
     1501 catccggagc tggcttaccg tgcagtcctg ggtctgctgg cacttcagaa aaaatacggc
     1561 ccggaacggc tggaaaaggc ctgttacgtt gccctgcact ataacgcccc ggacagacgc
     1621 tttatcgaca atctgctgcg tcatcatcgg gataacg
//
LOCUS       DAZPLI010000327         1655 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZPLI010000327 DAZPLI010000000
VERSION     DAZPLI010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.330"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..827)
                     /locus_tag="ACHUU3_004446"
     CDS             complement(<1..827)
                     /locus_tag="ACHUU3_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR2084312.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPKSFTAHNRAQAVFLC
                     VMHSHIQIMVGRAGQSQDWPGSVMTGCSNPVRLTTHEIATSGGELIKFIAEAAIMATT
                     LSHPCVTVENGRAVTTSVAVAEFFHKRHDNVLRAIANIECSDKFTALNFEASEYTDSI
                     GRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMIPG
                     DGRTLVVHFDKFGNVEFTETVPDGALVCPLETFRFYLEKQGWTLVNRGAIKNMTVEQ"
     gene            complement(1574..1655)
                     /locus_tag="ACHUU3_004447"
                     /pseudo
     CDS             complement(1574..1655)
                     /locus_tag="ACHUU3_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415662.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          409 a          410 c          393 g          443 t
ORIGIN      
        1 aattgttcca cagtcatatt tttaattgcg ccccggttaa caagagtcca tccctgtttt
       61 tccagataaa accggaaagt ctccagggga cagaccagtg cgccatcagg aacggtttcg
      121 gtgaattcga cattgccgaa tttgtcgaag tgaacaacca gagtgcgacc atcacccgga
      181 atcatcttgt cagcaggtgg ggtgttattc tggcgcagtt cggcctccat gcggtcgaac
      241 tcagcaatgt aggcttcttt gaatgcagcg gcttttttgc cagtgaagcc cataaccagg
      301 aaaacgaagc cgtttttggt gatttggtac attgggagtt tgcgcccgat tgagtcggtg
      361 tattcgctcg cttcaaaatt gagggcagta aatttatctg agcattcaat atttgcgatg
      421 gcacgtaaca cattgtcgtg tcgtttgtgg aagaactctg caaccgcaac agacgtagtg
      481 acagcgcgac cgttttctac ggttacacaa gggtgagaaa gggtagtagc catgatggca
      541 gcctccgcga tgaatttgat taactcacca ccggaggtag caatctcatg ggtggtgaga
      601 cgtacagggt tgctacaacc ggtcatcacg gaacccggcc agtcttgcga ctgccccgca
      661 cgccccacca taatttgaat gtggctgtgc attacgcata aaaaaaccgc ctgagcgcgg
      721 ttatgcgccg tgaatgactt cggggtagca atcccggcac ccgttttatg aggtgcaggt
      781 gcactataat tccacccgtt ctggttttca atagctacat tcaacatttt ctctttcctt
      841 tcatcaccga agtgaacttt gttgatgcgg tgcctggtgc ctccaggtga cgttaaccag
      901 ttaacaatta acgccggaat aagggatttt ccttactgtt ttaactgttc cgcgtgcgct
      961 gagccgcatt caccgcatca caaaattcac tttaaaaagg gcggacatca gccagcaatt
     1021 aaaccgatgc cgccaactgg tacttcacac agcaatgtcg ttatttacaa ccggaggcgc
     1081 actcccacca tttaaattta acagacaaga ccgactcttt atggataccg gaaatgcgcc
     1141 ttcgtgttgt gcccggtttt atttcaccac ctccgggctt tggtggtatc tttactgaag
     1201 ttctcacaca accagtaagg aaatgaatat gccaacgtat ctcgccagag tagaactata
     1261 taatgctgag ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag
     1321 gagaacgatc ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc
     1381 cgaaaagggc ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact
     1441 atccaggtat cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata
     1501 ccccgccaag acccggtgaa tatcctgtac ggccttctgc tttgtagaag gcttcttcgc
     1561 tatcataatc gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg
     1621 catttggctc cagttgctgg aggcgttttg catct
//
LOCUS       DAZPLI010000328         1649 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZPLI010000328 DAZPLI010000000
VERSION     DAZPLI010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.331"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..593
                     /locus_tag="ACHUU3_004448"
     CDS             <1..593
                     /locus_tag="ACHUU3_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFR2084313.1"
                     /translation="KIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAMS
                     EKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYY
                     ISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAIN
                     ILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            604..1041
                     /locus_tag="ACHUU3_004449"
                     /pseudo
     CDS             604..1041
                     /locus_tag="ACHUU3_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1041..1418
                     /locus_tag="ACHUU3_004450"
     CDS             1041..1418
                     /locus_tag="ACHUU3_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084314.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
BASE COUNT          556 a          247 c          419 g          427 t
ORIGIN      
        1 agaagataca aaaacaggga ggtgattatt tattcgcggt aaaaggaaac caggggcggc
       61 taaataaagc ctttgaggaa aaatttccgc tgaaagaatt aaataatcca gagcatgaca
      121 gttacgcaat gagtgaaaag agtcacggca gagaagaaat ccgtcttcat attgtttgcg
      181 atgtccctga tgaacttatt gatttcacgt ttgaatggaa aggactgaag aaattatgcg
      241 tggcagtctc ctttcggtca ataatagcag aacaaaagaa agagccagaa atgacggtca
      301 gatattatat cagttctgct gatttaaccg cagaaaagtt cgccacagca atccgaaacc
      361 actggcacgt ggagaataag ctgcactggc gtctggacgt ggtaatgaat gaagacgact
      421 gcaaaataag aagaggaaac gccgcagaat tattttcagg gatacggcac atcgctatta
      481 atattttaac gaatgataag gtattcaagg cagggttaag acgtaagatg cgaaaagcag
      541 ccatggatag aaactatctc gcgtcagtcc ttgcggggag cgggctttcg taatctttcc
      601 ctagggagtc agccgaatgt tgggaaggat ttgacggatg aagagaagaa ctgcttggcc
      661 ggcggcagta cgggaagccc gggaggatgg ggaccagaag atgagaatag cgcaagggat
      721 cgtatgcagc atgaacggta taaagatgaa ttgcgacaga aaatgggaaa acccgacgtt
      781 acagattcaa atttgaaaaa tattatagac gatctttatc ggccaaatgc aaaagtagga
      841 agtggaagca cagcagatgc tgttcgttat gaattggcca caggtgaaaa agttggtggt
      901 agaggacatg tattgaaagc acaggattat acaagggctt tacaggattg gttggataag
      961 aatccaacag catctccagg ggatcgagca gcagcagaaa acgttcttca ggatttacag
     1021 aatgctttgg gaggcaagta atgaaccaat atattgctag ttatatagaa aaaatggcat
     1081 cattttccac tgttttgtct gagacagtct cattagctaa tgaatactgg catccagaaa
     1141 cgccaccggt ggttatgttg ttctctcagg ttggaaaaac gattgtaaaa aatttttatg
     1201 aaatggggga tattgaaaga gaaaaaatat ttaaacatgt cgaagatggt atggtctcgc
     1261 ttgatgatga attggccaca gttgtagcta cagggcttat agaggcaata gtcactgcga
     1321 ccgatagcaa ttctttactt tggcaagatt taagtaaatt tctgggtgac aaatcaaagt
     1381 ctcatgcatt agtgtggaaa aattttggtc aggagtgata ttattcaaga agtattaagc
     1441 tcgctattga gatcttaatg cttcttgaga ttaaattaaa gttaggacga agtaattgca
     1501 ggtggaaata aatccatagt gacgtgtggc aatggctatt agatgttatg gtgtgatggg
     1561 ctttggtgat ttaagagcta agttagattg ccttatgaaa aagtatggtt atatatttaa
     1621 tgatgtgtat catatattta aataaaaaa
//
LOCUS       DAZPLI010000329         1630 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZPLI010000329 DAZPLI010000000
VERSION     DAZPLI010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.332"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..94
                     /locus_tag="ACHUU3_004451"
     CDS             <1..94
                     /locus_tag="ACHUU3_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR2084315.1"
                     /translation="RFYLEEQGWTLVNRGAIKNMTVEQLLKIHC"
     gene            105..293
                     /gene="dicB"
                     /locus_tag="ACHUU3_004452"
     CDS             105..293
                     /gene="dicB"
                     /locus_tag="ACHUU3_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFR2084316.1"
                     /translation="METLLPNVNTTEGCFEIGVTISNTVFTEDAINKRKHERESLNKI
                     CIVSMLARLRLMQKGCWR"
     gene            290..481
                     /locus_tag="ACHUU3_004453"
     CDS             290..481
                     /locus_tag="ACHUU3_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFR2084317.1"
                     /translation="MNTAFALVLTVILNTGEPAELVTGVYNSMKECVAAAAEQKIPGN
                     CYPVDKIIHQDNKEVPAGL"
     gene            575..>1630
                     /locus_tag="ACHUU3_004454"
     CDS             575..>1630
                     /locus_tag="ACHUU3_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFR2084318.1"
                     /translation="MSKVFICAAIPDEKATKEEGAKPVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESEPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMCPEIKLHAIGWIKHKCKPGAKWPEIQAEMRIWKKCREGERKETGKYTSVVDLARA
                     RANQQHTENSTGKISPAIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATD"
BASE COUNT          498 a          362 c          391 g          379 t
ORIGIN      
        1 ccggttttat ctggaagaac agggatggac tcttgttaac cggggcgcaa ttaaaaatat
       61 gactgtagag caattactca aaattcattg ttgaggacgc gataatggaa acgttattac
      121 caaacgttaa tacgactgaa ggttgttttg aaattggtgt cactatcagt aacacggtat
      181 ttactgaaga tgccattaac aagagaaaac acgaacggga gtcattaaat aaaatatgca
      241 ttgtttcaat gctggctcgt ttgcgtctga tgcaaaaagg atgctggcga tgaatacagc
      301 atttgcactc gttctgaccg ttattcttaa tacgggcgaa ccagctgaac tggttaccgg
      361 agtatacaac tccatgaaag aatgcgtggc tgctgcagct gaacagaaaa ttcccggtaa
      421 ctgttatccg gttgataaaa tcattcacca ggataataaa gaagtcccgg caggtcttta
      481 aagcagccac gtaataaata tccggtttca tttttatatg ccagcaatgg cagggatttg
      541 ttcaccctta aatctgtaat gaggttaaaa caaaatgagt aaagtcttta tttgcgccgc
      601 cattccggac gaaaaggcaa caaaagaaga aggcgctaaa cctgtagcca ctgccattga
      661 agccggcgac gaacgccgcg cccgcgcaaa atttcactgg caattcctgg aacattatcc
      721 ggctgctcag gactgcgctt ataaatttat tgtctgcgag gataaacctg gtataccccg
      781 ccctgccctc gattcctggg atgctgaata tatgcaggaa aaccgctggg atgaggagtc
      841 tgcttccttt gtcccggttg agactgaatc cgaaccgatg aacgtcactt ttgacaagct
      901 ggcccctgaa gtacagaacg ctgtcatggt taagttcgac acatgtgaaa acatcaccgt
      961 tgatatggtg attagcgcgc aggaactgtt gcaggaagac atggcaacat tcgacggaca
     1021 tatcgttgaa gcgttgatga aaatgccaga agttaacgcc atgtgtccgg agattaaact
     1081 gcatgccatc gggtggatta agcataaatg taagcctggt gcaaaatggc cagaaattca
     1141 ggcagagatg cgcatctgga aaaaatgtcg cgaaggtgaa cgcaaggaaa ccggaaaata
     1201 cacgtctgtt gttgatctcg cccgtgccag agccaatcaa cagcacactg aaaattcaac
     1261 aggaaaaatc agcccggcca ttgctgccat tcatcgcgaa tacaagcaga catggaaaac
     1321 actggatgac gaactggcct acgctctctg gcctggtgat gtggatgccg gaaacattga
     1381 cggcagcatc catcgctggg caaaaaatga agttatcgac aacgaccgcg aagactggaa
     1441 gcgtatctcg gcatcaatgc gcaaacagcc tgatgccctt cgctacgacc gccagactat
     1501 ttttggcctt gtccgtgaac gtccgatcga cattcacaaa gaccctgtgg cactgaacaa
     1561 atacattact gaatacctga ctacaaaggg cgtgtttgaa gatgaaggaa caaatcagag
     1621 cgcaactgat
//
LOCUS       DAZPLI010000330         1606 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZPLI010000330 DAZPLI010000000
VERSION     DAZPLI010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.333"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>1606
                     /locus_tag="ACHUU3_004455"
     CDS             <1..>1606
                     /locus_tag="ACHUU3_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HFR2084319.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGS"
BASE COUNT          558 a          314 c          303 g          431 t
ORIGIN      
        1 atgtatataa tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa
       61 tcaatccaga acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat
      121 cacaatgtga aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg
      181 ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata
      241 tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa
      301 agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg
      361 gagatttgca ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca
      421 ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt
      481 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      541 attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac
      601 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca
      661 tctctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc
      721 ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta
      901 acttatttaa cccaaaatat cataaaaaag ccgttatgaa ttacatggaa tatctggtaa
      961 cttgtcagtt ggatgaacaa caaatgtcat cactgcttta tgaaagagat gatttaagcg
     1021 ccattgattt ttcattgctc atgaagacga taaaagcctt ttcattcggg ggagatctcc
     1081 taacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcagaa agacggatat
     1141 tgattaacat taaccacgat tttcctaaca atggcaatct tttcaatgac tttctattta
     1201 accatcaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcagac
     1261 ctttagttta ctgggatggc caggtattaa aatcaacgac tgaaattaaa aattatgatg
     1321 ggtccacgcg tgtcggtggt gaggcgtggc ttaataaagg ttgctctacc tatcagcagc
     1381 tctacaatga attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg
     1441 gtgtatttgc aactaaaaac gttctggcct ttagtcgtgg ggattctgtg aacattaatc
     1501 actctcctca tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtacct
     1561 ggcctaacaa attgcaacct ggaatatata aaaacatggg gagtaa
//
LOCUS       DAZPLI010000331         1590 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZPLI010000331 DAZPLI010000000
VERSION     DAZPLI010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.334"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            190..1233
                     /locus_tag="ACHUU3_004456"
     CDS             190..1233
                     /locus_tag="ACHUU3_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR2084320.1"
                     /translation="MNDRLCFEVHDNKGYFVFSDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
BASE COUNT          453 a          354 c          338 g          445 t
ORIGIN      
        1 ttagcatttc aatcatctga catcattata aaaaatgaga ttcacataaa gataaaatta
       61 ttgttctcaa ggaaagcatg aaggggcttc gtaagcaata ttcattcggt gccaagacag
      121 gacttgatac acagaatctc aacttttatg ctctcagact atatcaccac atgttttaca
      181 ggctcagaaa tgaacgatag attatgcttt gaagtgcacg ataacaaggg ttatttcgta
      241 ttttcggata cctggtttgg cccattgctg ggggagtttg aagaagtgct tgatgcatac
      301 gacgctgatg aaatcagcga gacgagttac atcaacaaac tccgacgcct tgctcaacgg
      361 gagccggact ttattgatat acacgctcat ctggcatatg catttctgga acagaatgcc
      421 ccgcgtaagg cactgaacgc ctctcttaaa ggactggcgg caggtaatcg tatcatcccg
      481 gaaagtttct gtggtgaaat aatctggatg catcccgaaa accggcctta tctgcgcgcg
      541 ctttatgcag caattctcgc aaatgtgcac ttacagcgcc atcaggatgc ggttatgctc
      601 actgataaaa tactggcata caatcctgaa gataatcagg gggctcgctg gttgttgggt
      661 tcagagctgt tacgcaccgg cgatcatgag cgagccttca gtgtactgaa agagcatgct
      721 gatgaatttt caccttactg gtatgaactc ggtctcctgc attttctgaa cggggaacat
      781 gttaaggccg ccacagcttt tcgccatggc tttgccacaa acacttacat cgcagaaatg
      841 ctgtgcggaa atctgcatcc atttccactt gctgtgtggc atgacttctc cggctctctg
      901 gatacggcag aagattacta tgcaacatac tcaccattat ggggacaata tccggaagca
      961 ttgctgtttg taaactggct ctataaccat tcctccgtgc tgcatgaacg atcagaaatc
     1021 attaaatgcg ccgaaatgct gattcaggaa gatgatttcg aaatatgcga aagtatttta
     1081 agacagcagg agcacctgtg gaaaagaatt gacaagacac tttctgagga aattgtacag
     1141 aaatgcagaa atatgaacgg tgaatatatc tggccctgga tattgccctt ttccgcagca
     1201 gggatgaaac attcaagcat ccagtatcag tagatattgc attagtgtaa ctttaaaaca
     1261 agtaataatt tattatccaa taataacact gtgttccttc cccaggtgat taccctgggg
     1321 ttattttctg aaagtttcct ttgcaaaagg atagcgttgt aaattacttt aacgaattac
     1381 tccatgaact cattaaagtt ctttcgtaat actttatcgt caaacacgtt atagctggca
     1441 tcaaaaacca cagatttacc ctgccagcgg gtaatcccct cctctggcgt tttcggttga
     1501 actggtatcc ccgtggattt gcccctatat ttccagacat ctgttatcac ttaacccatt
     1561 acaagcccgc tgccgcagat attcccgtgg
//
LOCUS       DAZPLI010000332         1573 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZPLI010000332 DAZPLI010000000
VERSION     DAZPLI010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.335"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..1406
                     /locus_tag="ACHUU3_004457"
     CDS             <1..1406
                     /locus_tag="ACHUU3_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR2084321.1"
                     /translation="ELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQ
                     AEISSKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIA
                     LANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLIN
                     QRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIV
                     QFTAADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPL
                     TRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIRE
                     QASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQEL
                     NEYQQNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVK
                     QYLSHPVNTYTGVLLPK"
BASE COUNT          477 a          351 c          354 g          391 t
ORIGIN      
        1 atgagcttgc cgccaaaaaa gcattgtctc ttgcggctat cctggcgctg gaaaaagagt
       61 acgccaacaa aaagctggcg gcttacatct tcccaggcag aaatttatcg ttaacagtag
      121 acgctgaccc acaggcggaa attagcagca aagaaacgct ggctgagaat ctgacatcat
      181 taacactttc caatggtgcc agggttattc tggcaaaatc cgcgggtgaa gagcaaaagc
      241 tacaaattac tgccgtatct aataaaggcg atttaagttt ccctgcgcag caaaaatcac
      301 ttatcgcgct ggcaaataaa gcagtaagcg gaagcggcgt tggtgagctc tcctcttcca
      361 gcctgaaacg ctggagtgcg gaaaattcgg taaccatgag cagtaaagtc agtggcatga
      421 atacgttgct ctccgttagc gcgcggacta ataaccctga acctggtttt cagttgatta
      481 accagcgaat cacccacagc acgattaacg acaatatttg ggcatcgcta caaaatgctc
      541 aaattcaggc gttgaaaacg ctcgaccagc gtccagcgga gaaattcgcc cagcagatgt
      601 atgagacgcg ctatgctgat gaccgcacga aattactgca agaaaatcag attgtacagt
      661 ttactgccgc agatgcgctg gctgccgatc gccagttgtt ttcatctcca gcggatatca
      721 cgtttgtcat tgtcggtaat gtctcagaag acaaactcgt ggcgttaatt acgcgttact
      781 taggatcaat caaacactct gattcgccat tagccgcagg taaaccatta actcgcgcga
      841 cggacaacgc atcggttact gtaaaagaac aaaatgaacc tgtggcacag gtttcacagt
      901 ggaagcgtta tgattcccgg acacctgtta atctggcgac gcgtatggcg ctcgatgctt
      961 ttaacgtcgc actggcaaaa gatctacgcg ttaatattcg tgaacaggca tctggagcat
     1021 acagcgtttc ttctcgcctc tcggttgatc ctcaggccaa agatatcagt catttgctgg
     1081 cttttacttg tcaaccagaa cgacatgatg aactgttaac gttagcgaat gaagtgatgg
     1141 ttaagcgtct ggctaaaggg atcagtgagc aagaactgaa tgaataccag caaaacgttc
     1201 agcgcagcct cgatatccaa cagcgtagcg ttcaacaatt agcgaacact attgtaaata
     1261 gtcttattca atatgacgat cctgcagcat ggactgagca ggagcaattg ttgaaacaaa
     1321 tgacggtaga gaatgttaac actgccgtta aacaatatct ttctcatccg gtaaatactt
     1381 atactggagt attattgcca aaataataac agccccgtca acacatcgtt ggcggggatt
     1441 ttagcaatat tcgccatttt tatgtaataa ttttataaat gcgttcaaaa taataaccag
     1501 gtactaatag tgatatttta aggtctgatt tttacgtgaa aattcaggag gcacagaatg
     1561 cgcataaaaa taa
//
LOCUS       DAZPLI010000333         1549 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZPLI010000333 DAZPLI010000000
VERSION     DAZPLI010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.336"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            351..476
                     /locus_tag="ACHUU3_004458"
     CDS             351..476
                     /locus_tag="ACHUU3_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFR2084322.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPVLFFWG"
     gene            complement(921..1397)
                     /locus_tag="ACHUU3_004459"
     CDS             complement(921..1397)
                     /locus_tag="ACHUU3_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR2084323.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          358 a          405 c          386 g          400 t
ORIGIN      
        1 aaaaaaacaa ccacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctatgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctccatcgtg tttttgccgt ccgttcaccg ttaaaacgcc
      241 tcagaaatcg ggacaggatg tgtaatcgta taaccgcgca tgcacgccga tacgattacc
      301 acttggtcag ggcttcgccc cgacaccccg catcagtgcg taagtgctca atgtggcttc
      361 cggttctctt ccgcttcctc gtcgattcgc tcgctacgct cggtcatcga cttgcggcga
      421 gcgggatcac ctctcgcatg ttgtcggtga tcccggtgtt gttcttttgg ggatgagtcg
      481 ccagatagcc cctcaaacgc gctcaaaagc tctcagacgc acgaaacggg ttttagatgc
      541 ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaagcgcgc
      601 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcccc ctgcaaagcc
      661 catttgcacg gaacaggcag ccatgcggtt ggtaggtggt cgtagaccgt cggtgttgca
      721 gcgatggggg tctggggttc tccccagacg gtttccggta gcgctcaagg cgctgccgga
      781 aacgtggcgg ctcggttttt tctggagaac ggggagcaaa aacacgcaaa ggattttgag
      841 gcggcaaaag agcaatgcag gatagacaat catggtcaga acgttaacac gtagcccctg
      901 attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcggcg ctgaatatca
      961 ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt agtgttgtat
     1021 cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc aacggggatt
     1081 ctaactaatg cattttttga ttctaagtaa ttgagccacc gaataattgt gcttctgcta
     1141 actccaagtt tttcagctag gaattgctga tccgcaacga cagcgccaca cgtaggatcg
     1201 atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct atccttcagc
     1261 agctctcgaa cacgttccca accttttggg gatacctgtg taaaccttga atttttcttt
     1321 gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt cagtcgtttt
     1381 tctgaatcaa taaacatgat tttttccctc tgaaaatccc atgcactaac gttggttcaa
     1441 atctaagcca tcatgacaca tctgtgcaac accttgttgc gtgttgtcgc atccaggatg
     1501 cgacaacgtt gtgtcatcct ggatgcgaca ggtaaataaa aaacacact
//
LOCUS       DAZPLI010000334         1537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZPLI010000334 DAZPLI010000000
VERSION     DAZPLI010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.337"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>1537
                     /locus_tag="ACHUU3_004460"
     CDS             <1..>1537
                     /locus_tag="ACHUU3_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR2084324.1"
                     /translation="YEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRGAVAIDDAGYDE
                     DDFKKPVRVNLPVVGDLPPEGVFDTEFCNRYEKGGEDGITMVFIASSPSVQDKPASTD
                     NTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRDLHKLVRDTDKVFPNPGNSSLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQP
                     SCAMADEQATAETVEPDATEHHQDTQPLDAQSQVNAVDAKYQKLRTELHEARKNIPPK
                     NPVDADKLLAASRGEFVEGISDPNDSKWIPGHHISSNEVKKTENKVRQTEDKQHQNSE
                     PEER"
BASE COUNT          440 a          400 c          372 g          325 t
ORIGIN      
        1 ttacgaagca aaaaatgaca aagtaagaaa acgcctcggt attaaaggcg gtttttactg
       61 ggctgaagcg aaaaaattat ccattgccat ctcccgtggt gctgttgcga ttgacgatgc
      121 tggctacgat gaagatgact ttaaaaaacc tgttcgcgtc aatttgcccg ttgttggtga
      181 cctcccacca gaaggcgtgt ttgatacgga attctgcaac cgttacgaaa aaggcgggga
      241 agatggcatc acaatggtat ttatcgcatc ttctccctct gttcaggaca aaccagccag
      301 cactgacaat accaacgtca acggcgaaga catgacagaa attgaggaga acatgctcct
      361 gccagtttcc ggtcaggaac tgcccattcg ctggcttgct caacacggca gcgaaaaacc
      421 ggtaacgcac gtttcacgcg acggactcca ggcattacac attgctcggg ctgaagaact
      481 accggctgtt actgccctgg ctgtttccca caaaaccagc ctgctcgacc cgctggaaat
      541 tcgcgatctc cacaaactgg ttcgtgacac tgacaaagtt ttccctaatc ctggtaattc
      601 aagcctggga ctgataactg cttttttcga agcatacctg aacgctgact acaccgatcg
      661 aggactgctg acaaaagagt ggatgaaggg taatcgtgtt tcacacatca ctcgcacggc
      721 ttccggtgct aatgctggcg gcggaaacct caccgatcgc ggcgaaggtt tcgtacacga
      781 tctgacgtca ctggcgcgcg acgtagccac tggcgtactg gcccgttcaa tggatctgga
      841 catctataac cttcatccgg cacacgctaa acgcattgag gaaattatcg ctgaaaataa
      901 accgcccttt tctgttttcc gcgacaaatt catcaccatg cctggcgggc tggattattc
      961 ccgcgccatc gtggttgcgt ccgtaaaaga agcaccaatt gggatcgagg tcatccccgc
     1021 gcacgtcact gaatatctga acaaggtact gactgaaacc gatcatgcca accctgatcc
     1081 ggaaatcgtg gatattgcct gcggtcgctc ctctgccccg atgccgcagc gtgtaacaga
     1141 agaaggaaaa caggatgatg aagaaaaacc gcaaccatct tgcgcaatgg cagatgaaca
     1201 ggcaacggct gaaacagtgg aaccggatgc aactgaacat catcaggaca cgcagccgct
     1261 ggatgctcag tcacaggtaa atgctgttga tgcgaaatat cagaaactgc ggacagaact
     1321 ccatgaagcc cggaaaaaca ttccgcccaa aaatcctgtc gatgcagaca aattactggc
     1381 tgcctctcgc ggagaatttg ttgaagggat tagcgacccg aatgattcga aatggattcc
     1441 cggtcatcat atctcctcaa atgaagtcaa aaaaacggaa aataaagtgc gacagactga
     1501 agataaacag caccagaaca gcgaaccgga ggagcga
//
LOCUS       DAZPLI010000335         1525 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZPLI010000335 DAZPLI010000000
VERSION     DAZPLI010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.338"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            97..360
                     /locus_tag="ACHUU3_004461"
                     /pseudo
     CDS             97..360
                     /locus_tag="ACHUU3_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701870.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            complement(397..954)
                     /locus_tag="ACHUU3_004462"
     CDS             complement(397..954)
                     /locus_tag="ACHUU3_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR2084325.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(951..1226)
                     /locus_tag="ACHUU3_004463"
     CDS             complement(951..1226)
                     /locus_tag="ACHUU3_004463"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFR2084326.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            1412..1525
                     /locus_tag="ACHUU3_004464"
                     /pseudo
     CDS             1412..1525
                     /locus_tag="ACHUU3_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
BASE COUNT          424 a          325 c          367 g          409 t
ORIGIN      
        1 cgcagacacg tcgtatgcag gaacgtgctg cggttagttt gtgagctttc gatagtggtt
       61 gttatttttg cccttatttg ttccggaggc catggttcaa tggtccgtct gccccctgtg
      121 gtgatgtcag caaaatcagc cactgcgcga accacaatag cccgggaaga tgctgaagat
      181 caccaggtaa agctgtcagc gcagaaactg gaagaactgc tcgcatcaat ggttaaggat
      241 gaggttgatc gcaatgatgg gatttattga cgtcagcgag agcagaagga agaactgaat
      301 aacctgaatg atttacgctc aatcagagcg atggcgatta gcggcaatca cgctcgataa
      361 aattaacctc ggtgatcatc cggggttttg cattcattaa aaccgcatca acctttccac
      421 caattgttct ttacgggcaa cgatccaccc atgttgctcc agataaaatt taaaccgttc
      481 cagagtgcat accatcgcat cggcggggac tttttctgtg aactcgacct gaccgtgttt
      541 atcgaagtgg atcagtaatg cgcatccatt atttggggcg ggggagtttt gtgctgcagg
      601 aggacgtttg tggagttctt cttccattgc gttaaatttt tcaatgtaag cttcttttag
      661 tctggctgct tttttgccag taaatcccat tacgaggaac atgaagccgt cttttgtgat
      721 caggtagcat cgtgattcac gttctgcgcc gttgccaatt tcgacagtct gaacatcggc
      781 cgaaaagttg gctgatgtaa attcgggaga gcagtcgagt gtgtcaatct tagctaaaac
      841 gtgtttatgt tgtttatcga agtagtcggc aacagcttga gaggtggtaa ccaggcgacc
      901 atctttgaaa aagacttttg gtgactcgtt agaaaaagtt atagctgtat tcatatgtga
      961 tgactccaat taattggatg atcaccacct ctgacgccaa tcagggaggt ggtgagacgt
     1021 acagggttgg cgtaaccggt catcacaaaa cccggcgcgg atttctccgc ccctgcacgc
     1081 cccaccataa tgcgaatgtg gcaatgctta acgcaaaaaa aaccgcaaac gcggctatgc
     1141 gttgtgatgt tatccgagac gccaatcccg acaaccgatt ttgcggttgt gtgaggaata
     1201 tagccccgga gaacaaaaac tgtcaacggg caaaaaaatt tccgttatca ggaatgtata
     1261 aaattaaagc gatcgagcaa aaaatccttt ctgcaaaact aaaaaagatt tcaataaaat
     1321 caggcagtta aaaacacgga aaaacggtat aaaaaagcgc ctcaaaatcc tgttttttct
     1381 ttcagataca atgtcttatt gcatttttta aaacatgtca gtgtggtaca tggatatcga
     1441 taccatcgcc aattataaac tgggcggtgc acagcaacat gatagcgtac gcctcgatcc
     1501 gtgggtgttt atgttctcag cagga
//
LOCUS       DAZPLI010000336         1518 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZPLI010000336 DAZPLI010000000
VERSION     DAZPLI010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.339"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..1104
                     /locus_tag="ACHUU3_004465"
     CDS             <1..1104
                     /locus_tag="ACHUU3_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR2084327.1"
                     /translation="LAWAVFKIFRIPVNQWTLATAALGGVFLVSGLILLMNYNHPYTF
                     TAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTA
                     THNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNF
                     LAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGY
                     VTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPG
                     QVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYA
                     QVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT          379 a          338 c          392 g          409 t
ORIGIN      
        1 ctggcgtggg cggtctttaa aatctttcgt attccggtga atcagtggac gctggcgacg
       61 gcggcgctgg gaggcgtgtt tctggtgagt ggtttgattt tgttgatgaa ctacaaccac
      121 ccttacactt ttaccgcgca aaaggcagtg atagcgatcc ctatcacgcc acaggtgacg
      181 ggaattgtta ctgaagtcac tgacaagaat aatcagctta ttcaaaaggg cgaggtgctt
      241 tttaagctcg acccggttcg ttaccaggcg cgagttgaca gacttcaggc tgacctgatg
      301 acggcgacgc ataatataaa gacgctgcgt gcgcagctca ctgaagcgca ggccaacacc
      361 acccaggttt cagcggagcg cgaccgtctg tttaaaaatt atcaacgtta cctgaaaggc
      421 agccaggcgg cggtgaatcc gttctcggaa cgtgacatcg acgatgcgcg gcaaaatttc
      481 ctcgcgcagg atgcgctggt gaaaggctcg gtggcggagc aggcgcagat ccagagccag
      541 ctcgacagta tggttaacgg cgagcaatcg cagattgtga gcttaagagc gcaacttact
      601 gaagcaaaat ataaccttga gcagactgtc attcgcgcgc cgagcaatgg ctacgttact
      661 caggtactga tccgcccagg tacatacgca gctgccttgc cgctgcgtcc ggtgatggtc
      721 ttcatccccg agcaaaaacg gcaaattgtc gcccaatttc ggcaaaactc gctgttacgt
      781 ctgaaacctg gcgatgatgc ggaagtggtg tttaacgcgc tacctgggca ggtgttccac
      841 ggcaaattga ctagtatttt acctgtcgtg ccaggcggtt cttatcaggc gcagggggta
      901 ttgcaatcat taacggtcgt tcccggcacg gacggcgtgc tgggaaccat tgaactggac
      961 cctaacgatg atatcgatgc cttacccgac ggcatttacg cccaggtagc agtttactcc
     1021 gaccatttca gccatgtttc ggtgatgcgg aaggtgctgc taagaatgac cagttggatg
     1081 cattatttgt atttggatca ttgaggggga agggggagat tatggaactt tgctccctgc
     1141 aagggctaat tatttttgca tattacttca ttggctgcga tgatttttct ttaatattca
     1201 ttatgttaat aagttctgga tgagtgtgct ctcattggat tttttatccg tcttgttctg
     1261 ccgcaggatg agactataca tcgtcgagat cgcaatgatg atatgagttg ttcctcctgc
     1321 gacggctcta atcaaaaaag agcaggagtg cttttccatc tattgcgatg atggctttat
     1381 tatctgaagg atgacagtca catttaaaaa atatttaatc tatagatcac aatgtagggt
     1441 catggaatat atgtatacaa ctattttctt cttcactttt aattatcgta ttcattataa
     1501 aactaagtta aaaaaact
//
LOCUS       DAZPLI010000337         1482 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZPLI010000337 DAZPLI010000000
VERSION     DAZPLI010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.340"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="ACHUU3_004466"
     CDS             complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="ACHUU3_004466"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR2084328.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1092..1460)
                     /gene="cadB"
                     /locus_tag="ACHUU3_004467"
                     /pseudo
     CDS             complement(1092..1460)
                     /gene="cadB"
                     /locus_tag="ACHUU3_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="antiporter protein responsible for lysine import
                     and cadaverine export; member of the lysine-dependent acid
                     resistance system 4 (AR4); inner membrane protein;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
BASE COUNT          413 a          324 c          371 g          374 t
ORIGIN      
        1 tggtgtaagg cacccacgcg gagtcaaagt ggatggattt cacatccagt gttttcttga
       61 tgaagtcggt gttgtacagc agaccatcat aggtagagtt ggtaattaca gcatgtaccg
      121 gccaggttgc gtttggtgtt tctttcacgc gcttagcaat ggtagcgtgc tggaattcac
      181 tctgtgggat accaccaaga ataccgtaag cgttacgggt cgggcggaaa tagattggcg
      241 taacatcgct catcatcatc aggtgggtca gcgatttgtg gcagttacgg tcaatcagaa
      301 tggtgctgcc tgccggagca gagtacatgc caacaatttt gttcgcagtg gaagtaccgt
      361 tggtcaccat gtagctgcgg tctgcgttaa agacgcgagc gatatactgt tctgcttctt
      421 tgtgtggacc actatgatcc agcagagaac ccagttcaga tactgaaatg gaaatatcag
      481 atttcatggt attcggacca aagaaatcat agaacaggct acctaccggg cttttctgga
      541 atgcagtacc gcccatgtga ccaggagtac agaaagtata tttaccttca cgaacatatt
      601 taaacagtgc tttagtcagc ggaggcagaa tagtgttgat atattcgtca gtggtctgct
      661 tgatcttatt agcaatatct tcagcagcac ccagcgcata ttcaaagaag ctaatctgta
      721 aacgcaggtc attcaggctt acatcgagag tggaatacgt attagcgaac gcgtacaacg
      781 gcaggttctc gttcattttg ctaatttctt cgcacagctc gagattatat ttatcccagt
      841 caaaaataac gccgcacaga cgcgcattgt tttcgatcag ttttaataag tcgtcacggt
      901 cgttcgggta aacaatctgg aagttcagac gttcaagcgc gcgatgaagt tcacggatgg
      961 gttcttcttt aaaataaacc cccatgtgat tcaatattgc aataacgttc atagtcatat
     1021 ctccaggtaa aaaaggcccc tcccaacaca tgggacaaaa tgaaaggagg agcctcggaa
     1081 aatactttta attaatgtgc gttagacgcg gtgtggttat ccattgagtg gctctggcgc
     1141 tcgtgcattt tgcgagcgta gaacatcagg ataatcaggc tgacgatgaa ggtacctgcc
     1201 agctcgaagg agcttgcgcc catcagcgcg atgaagcaga acacacaacc cagtacagag
     1261 cagatcaggc tgacaaagtt gcggatgtta acgccttcaa aacgaatcag gtcaacgcaa
     1321 gagtagaagt acggcagcat agtcagcagt actgcgatac cggtcagttc accgaacagg
     1381 tcagatgctt taccaccggc agagttcatc agagtgatga ggatcatcag ggcagtcatt
     1441 ttcactgcag ccagcagcag taatgccgat cagttaagga tc
//
LOCUS       DAZPLI010000338         1480 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZPLI010000338 DAZPLI010000000
VERSION     DAZPLI010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.341"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            289..1404
                     /locus_tag="ACHUU3_004468"
                     /pseudo
     CDS             289..1404
                     /locus_tag="ACHUU3_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          580 a          278 c          213 g          409 t
ORIGIN      
        1 cacatctaaa acatcacaaa aaaaatcact tcacacacta tcagagaaca taataacttt
       61 ttaaaatgag cgcattttga tagttgttaa cgatttcctt tacgatacta tcactaccct
      121 ttttttatac acaaatatta ccgggtggag ataaaaggga aatcaaaggt aattatatca
      181 ggaagatata acgcattaca tttattgtgt gtagaagaaa atccattcct gttctaatta
      241 tccaatttaa acatcttagc ataaaacaaa tgatgaataa ggaattttat gtcaattgac
      301 tttaccccag gtataataaa tacatatcac ggcgatattt ataactgcac aacaaatacc
      361 gataatgcta aaacgccaga cacaccgaag tggccttgcg ataattggga agaacaacaa
      421 cccattaatt ccacattctc tggagaagga tatatctctg atcaatatga tttagtgcaa
      481 caccaacttc aacaaattaa tgcgtgccac acgaacacta catatacaaa tgcagactac
      541 tcaaaagttg tggctcaact tgtaagtctt attactaaca ttgaaacgat aagttcgaca
      601 caacttacgc aacagacaca atcaatactt aatcagatta ataacattag atatgaaaaa
      661 aacaaaagtg ctgaatgtcg aatcattgtt gtcgctaatc ctaaacccga caaggcaatc
      721 ataactaaaa ttagcgtaga agagggaata cccataacat tcagtgtcca aaccatgttt
      781 tcagatacta attttattgc tgaacaacga gctgacttac caactaatat aaaagatatt
      841 caaagcctgt atcaaaaaat gacgaagtta tatattgagc acagtgaaaa taaaaacagg
      901 atgaaagtct ttgccggagc caactttatt gatttcaata tgacaggaca gaacctgtcc
      961 gggttcgttc taaccttgtc aaggttttat ttcgaagatc tacttaatat aaatttcact
     1021 gacgcaaatt taggtgacac tattttttac ataaagaaca ccctaccccc aaattatata
     1081 aagatggaca atatcttgac aaacaaatcg aaggtttatt ttcaacatta ttaaccatta
     1141 atgacaactt tttacgagca aaagcggaaa ttgcttcgac aataattaaa tttttagagg
     1201 ccaggataac caatctttca tacaatgaca tattaaaata tcagcaagaa ttccaaaaac
     1261 aatgttataa gcaagttaaa gcctttacga cactctctcg atacaataaa attcaaacat
     1321 gggcagaaat gtctgaatat cagttcgaag tatttcaata cgagacgctt aaccccaaaa
     1381 aaatgtcaca cacgccttat ttaaagaggc cacttcctaa tgaaaaagat ataaattatg
     1441 gggtggaaat agaaatacca tctggcaaaa gaattcgctt
//
LOCUS       DAZPLI010000339         1443 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZPLI010000339 DAZPLI010000000
VERSION     DAZPLI010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.342"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..1117
                     /locus_tag="ACHUU3_004469"
     CDS             <1..1117
                     /locus_tag="ACHUU3_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR2084329.1"
                     /translation="TWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELAVMLGRLERELRAGAVSAESEAWLAQCGLTVEQMES
                     QMEAEYIPERRLHLYHCDHRGLPQALISPEGETAWRGEYDEWGNQLNEENPHHLYQPY
                     RLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGINLYTYPLAPIRYTDPLGL
                     ERVISVYGPPAPDRAGAETPLVLTDMTGGVTIYYDPETGDSMTFDSSNRIDRRSQRGA
                     GDPYTGEVVGCETNGSGISAAYGTTKIYTTDTRARWLHGGGSSLRDPYAPRQGWKPTM
                     GCTRAQNEDVDELCKKVTSWMYSHPGERIRYERFKTR"
     gene            1095..1334
                     /locus_tag="ACHUU3_004470"
     CDS             1095..1334
                     /locus_tag="ACHUU3_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084330.1"
                     /translation="MNVLRHVNAINMGVCILILVIGCANAKVLKRCILPAPDGGVEKL
                     SKPNLHGVIKKVDLNTNEAEIKIKEGAPIKLSSML"
     gene            complement(1329..>1443)
                     /locus_tag="ACHUU3_004471"
     CDS             complement(1329..>1443)
                     /locus_tag="ACHUU3_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084331.1"
                     /translation="MSQGKRHNQALIALARRRCDVLFAMMRDGTFYTPQGS"
BASE COUNT          386 a          302 c          439 g          316 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacact gatccactgg ggttagaacg
      661 agttataagt gtatatgggc ctcccgctcc agaccgggca ggtgctgaaa ctcctcttgt
      721 tcttactgat atgactggag gagtgactat ttattatgat cctgaaacag gtgattcaat
      781 gacttttgat agtagcaata gaattgatcg tcgttctcaa cgtggtgctg gtgatccata
      841 tacaggtgaa gtcgttggtt gtgagacaaa tggatcagga atatcggcag catatggcac
      901 aacgaagatc tatactacag atactagagc aagatggcta catggtgggg gatcatcgtt
      961 gcgcgatcct tatgctcctc gacaaggctg gaaaccaact atgggatgta cccgtgcaca
     1021 gaatgaagat gtagatgagt tgtgtaagaa agtgacaagt tggatgtatt cgcatcctgg
     1081 agagagaatt agatatgaac gttttaagac acgttaatgc gattaacatg ggagtgtgca
     1141 ttctaatttt ggttattgga tgtgcaaatg ctaaagttct taaacgatgt atattacccg
     1201 ctcctgatgg tggcgtagaa aaactatcaa aacccaactt acatggagta attaaaaaag
     1261 ttgatttgaa cacaaatgaa gcggaaatta aaataaaaga gggtgcccct attaagttgt
     1321 caagcatgtt atgacccctg cggggtataa aaagtcccgt cgcgcatcat ggcgaacaga
     1381 acgtcgcagc gtcgtctcgc cagggcgata agcgcctgat tgtgtcgttt tccctgactc
     1441 att
//
LOCUS       DAZPLI010000340         1426 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZPLI010000340 DAZPLI010000000
VERSION     DAZPLI010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.343"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHUU3_004472"
     CDS             complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHUU3_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR2084332.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHUU3_004473"
     CDS             complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHUU3_004473"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR2084333.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(822..>1426)
                     /locus_tag="ACHUU3_004474"
     CDS             complement(822..>1426)
                     /locus_tag="ACHUU3_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR2084334.1"
                     /translation="GDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCF
                     GKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADE
                     EALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPG
                     AEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          323 a          397 c          396 g          310 t
ORIGIN      
        1 tatgttgatc aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg
       61 gaacgaaggg ggccagcggt gcgctgctgc cagcgggaat aattacggat tttggcatcg
      121 ttaaactcct taagcgatac gagcaaagga cgtgggagaa agcgcgtcgc agaacgtttc
      181 gacgtcgctg acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa
      241 ttcaggcccc gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa
      301 gccatcgcgt agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg
      361 gcgtattcca cggctgcgca aaatgctgtc cagcggcgta ttgaagaaac cgctgtagcg
      421 cggcttcggc agcacaatat cgccaggctg cggcaccagt tcatccacca gttgataatc
      481 ccaggagcct ttcgccagca atttcccctg cagctgcggc tgcttacgca tggttttcag
      541 ggcgttcgat ttatgaaaat tcggtgagcc gggtccgcca gcttcgacat actgttcatc
      601 ccagccattt tgaaaccaga tgatcagcat ccctgccgct cgcgctgcgg tcacggcggt
      661 ttgaatgttg gcaatgaccg ggcgagtggt tgagacatca aacccggcga gatctaagta
      721 gccgcctggc gtggcataag cgttttgcat atccaccacg atcagcgcac tttgctgcgg
      781 atcgaaggta atggcttccg gtcgagcggt taaggtcgtc atcatgccac ctcctgagtc
      841 agcgcaggga gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaaggtttcg
      901 attcccgaca gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg
      961 acttcatcta acatgcgcgc gacactggcg taagaaccga ctaatgtccc catattgatg
     1021 tttaccgccg aagtgggatc ggccatctga cggacgttgg tgtcagtacc ggagcgggta
     1081 tctttctgac tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag
     1141 tgttcccatt tggcgcgagc ggcatcgtcg gtttcatcgg caatcaccat aaacaacaca
     1201 taagagccaa cgtcgcgccc ggtttgctct gcggcctgtt tcatccgcac agcggtcggg
     1261 gcgaaagccg tgggtgtatt tacgcctttg ccgaaacaga agttgaaatc ggcatactga
     1321 gcggagaacg ccatgccagc gtcgctttgc ccggcgcaga tcactttcat ggggacactc
     1381 ggttgcggac tgacgcgaca atcattcatg gtgaaaaaat cgcctt
//
LOCUS       DAZPLI010000341         1421 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZPLI010000341 DAZPLI010000000
VERSION     DAZPLI010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.344"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>1421
                     /gene="rhsC"
                     /locus_tag="ACHUU3_004475"
     CDS             <1..>1421
                     /gene="rhsC"
                     /locus_tag="ACHUU3_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HFR2084335.1"
                     /translation="LVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIR
                     LSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVA
                     HRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAG
                     LKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRAS
                     AFYYNHHSQLTSATGPDGLEMRRKYDESGRLIQETAPDGDIIRYRYDNPHSDLPCATE
                     DATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQ
                     LIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYD
                     AAGRVIRLTSENGSHTTFRYDVLDLLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTH
                     WHYDEADRLTHRTVNGETAERWQ"
BASE COUNT          338 a          422 c          436 g          225 t
ORIGIN      
        1 ctggtactga ccacgcaggc gcagcgggca gaagaagccc ggcagcaggc catttccggc
       61 gggacggaac cgtccgcttt tcctgatacc ctgccgggtt acaccgaata tggccgggac
      121 aacggcatcc gtctgtctgc cgtgtggctg acgcacgacc cggaataccc ggagaattta
      181 cctgccgcgc cgctggtgcg ctatggctgg acgccgcgcg gcgaactggc ggcagtgtat
      241 gaccgcagca atacacaggt gcgcagcttt acttacgatg ataaataccg gggccggatg
      301 gtggcgcacc gtcacacggg ccggccggaa atccgttacc gttacgacag cgacgggcgg
      361 gtgacagaac agctaaaccc ggcaggctta agctacacgt atcagtatga gaaggaccgc
      421 atcaccatca ccgacagcct gaaccgccgt gaagtgctgc acacgcaggg tgaagccggg
      481 ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg tcacgcagag tcagtttgat
      541 gcggtgggca ggctcagggc acagacggat gccgcaggcc ggacgactga gtacagcccg
      601 gatgtggtga cgggactcat cacgcgcatc accacgccgg atggcagggc atcggcgttt
      661 tactataacc accacagcca gttaacgtca gccaccgggc ctgacgggct ggaaatgcgc
      721 cgcaaatatg atgaatccgg ccgtctgatt caggaaactg cccctgacgg cgatatcatc
      781 cgctaccgtt atgataatcc acacagtgac ttaccctgcg caacggaaga tgccaccggc
      841 agccggaaaa ccatgacgtg gagccgttac ggtcagttgc tgagcttcac cgactgttcc
      901 ggttatgtaa cccgttatga ccatgaccgc ttcgggcaga tgacggcggt gcaccgcgag
      961 gaagggctga gtcagtaccg cgcatacgac agccgtggac agttaattgc cgtgaaagac
     1021 acgcagggcc atgaaacgcg gtatgaatac aacgccgccg gtgacctgac caccgtcatt
     1081 gccccggacg gcagcagaaa cgggacacag tacgatgcgt ggggaaaagc tatctgtacc
     1141 acgcagggcg gtctgacgcg cagtatggaa tacgatgctg ccggacgggt catccgcctg
     1201 accagtgaaa acggcagcca caccaccttc cgttacgatg tactcgacct gctgatacag
     1261 gaaaccggct ttgacggccg cacacagcgt tatcaccacg acctgaccgg caaacttatc
     1321 cgcagcgagg atgaggggct ggtcacccac tggcactatg acgaagcaga ccgcctcacg
     1381 caccgcaccg tgaatggcga aaccgcagag cggtggcagt a
//
LOCUS       DAZPLI010000342         1392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZPLI010000342 DAZPLI010000000
VERSION     DAZPLI010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.345"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..459
                     /locus_tag="ACHUU3_004476"
     CDS             <1..459
                     /locus_tag="ACHUU3_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR2084336.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            477..1115
                     /locus_tag="ACHUU3_004477"
     CDS             477..1115
                     /locus_tag="ACHUU3_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR2084337.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          445 a          282 c          306 g          359 t
ORIGIN      
        1 ttgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac
       61 gaattacttc ccgccagcct agatgcagca aaagcccatg gactgaccga acgcgatgtt
      121 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      181 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      241 ttactgactc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      301 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttca
      361 ttacccgatt tctcacatta tgaactgtat gacaaaccgg aagcagttca ggaggcgctg
      421 gaaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      481 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      541 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
      601 cctatccaac taatagcaag attattatgg gagatgtact gaatcatgca gcactgaagc
      661 aggccatgca gggacaggac gttgtgtatg ccaaccttac gggggaagat ttagatattc
      721 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      781 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      841 ttggtgagcc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
      901 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
      961 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1021 tcacagatat catcgataaa cctgagaaac acattggtga gaatataggt atcaatcaac
     1081 ccgtgactga tggtgacaag ccattattca tgtaaaccaa ttaaatagtt accatcgtaa
     1141 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tccgatgcga
     1201 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacttaca
     1261 tctaagtcag cataattcta atatctgcac atcaataaaa atgcaaacaa cttccggcaa
     1321 aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata atccgcaaaa
     1381 tttgcacaac ac
//
LOCUS       DAZPLI010000343         1384 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZPLI010000343 DAZPLI010000000
VERSION     DAZPLI010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.346"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..1339)
                     /locus_tag="ACHUU3_004478"
     CDS             complement(<1..1339)
                     /locus_tag="ACHUU3_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR2084338.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSR
                     LTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLM
                     TLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          281 a          391 c          357 g          355 t
ORIGIN      
        1 ctgactggct ctttttcggg gctgtggggt atttccaggg cctctccttt accactctcg
       61 ggaaggccct ttcccttctc gtcggtaatt tcacaagttg tcccatactt gcaagatcgc
      121 gcatcagctc cggtatacgt cccggtgaag cgccctgcaa tgtcatcagc attctcatca
      181 ccattccgca tgattctgag aaactcagtt gattcggcca gtaacctttc agatgttccg
      241 ccattttaat catctgatat ctcaccagat tataagccag taagacaccc cacagctctt
      301 gctccacaag ctccggcttt ttacttctca gcgtcagcct gctcagttgc atcgtctgtt
      361 ttatctccct gtatcccagt tcgatttccc agcgatgact gtacagatcc gccatttctc
      421 ctccggggaa gcgcatggcg tccgtcatcg acgtcagcag atggcagact tttcctttgc
      481 gcgtcacggt cagcaggcgg gctgtcactt catttcccag tcccggccac ttttttcgtg
      541 cctgcgggct ggttttcagc ttcaccagat gatcgccttt acccagtttt ctgagctctt
      601 catattgcgc tccctttctg agaggtatca tccagtggcg gtgttctccc gccaggctcc
      661 aggcatttaa cagtcccagt gagtaataac ctttatccat taacgtcaga gtgttatcgc
      721 cggtttgttc tataagttgc tcagcaagct cattttcgct gttcttcatc gtgccgaagg
      781 ctgcagccgt cagcagatgg ctggtcagtt ccatctggca gaccattttg acctgcgggt
      841 agagcgccgg gttcccggca tgtgtctggc gggggaaggc tgcatcgttc tctggtgtat
      901 ccggtgtgcg ccagaacaca ccatcgatgg ccagcagggt caggccgcac cagtgcggat
      961 gcggcgtggc gttatgccag agctgcgctg ttttcgtgaa cacgcggcgg acagcctcac
     1021 ttcccaggcg ctggcgggcc tgaataacgg cactgggggc aacgaagggg cgattgcccg
     1081 gcagcatgat gtccaggcga ttcacaatct ggtgaagagg ttctttacgc tcaagcgcca
     1141 tgccaacaat acaccagacc atcatttcga ggggaagacg gcgcttgcgt agcgttacag
     1201 tacctgattc ggcaaggcaa cgagagatga gttcggggtc gaggtaatcc cccagagaag
     1261 tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt ccaatgtgca
     1321 taaaaaaatc cggaaacaag tgagcgtttc cggattctta cacagccact ggatcggtca
     1381 actg
//
LOCUS       DAZPLI010000344         1377 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZPLI010000344 DAZPLI010000000
VERSION     DAZPLI010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.347"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..984
                     /locus_tag="ACHUU3_004479"
     CDS             <1..984
                     /locus_tag="ACHUU3_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR2084339.1"
                     /translation="LVVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVR
                     SIDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTP
                     PGYTGMGEPLPVLYFYHSFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETD
                     AKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQ
                     GGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGD
                     KDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            999..>1377
                     /locus_tag="ACHUU3_004480"
     CDS             999..>1377
                     /locus_tag="ACHUU3_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR2084340.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT          360 a          315 c          362 g          340 t
ORIGIN      
        1 ttagtggtgg gtgttccggt tccggacaat attcacccga tgatcaaaga cgaagcagga
       61 gtctggtcgt ggcgcacgcc tgtcctgaaa ggcaacctgt atgagtattt tttcaatgtt
      121 gatggtgtac gcagcattga tataggcacc gcaatgacta agcctcagcg ccaggttaac
      181 tccagtatgg ttctggtgcc aggcagttat ctggatacgc gttctgttgc gcatggtgat
      241 ttgatcgcca taacttacca ctccaacgca ttgcaatctg aacgtcagat gtatgtctgg
      301 accccgccag gatacaccgg catgggcgag cctttgccag tgctctattt ctatcacagt
      361 tttggtgata ccggacgttc cgctatcgat caggggcgta tcccgcaaat catggataac
      421 ctgctggctg aagggaaaat taaaccgatg ctggtggtga tcccggatac cgaaaccgat
      481 gcgaagggca ttattcccga agatttcgtg cctcaggaaa gacgtaaagt cttttatccg
      541 ctgaatgcta aagcggcaga tcgcgaactg atgaacgata ttatcccgct gattagcaag
      601 cgttttaatg tccgtaaaga tgccgatggt cgcgcgctgg cagggctttc acaaggcggg
      661 taccaggcgc tggtttccgg aatgaatcat ctggaaagct ttggctggct ggccacattc
      721 agtggtgtta ccacgacaac cgtaccggat gaaggtgtcg cggcccggct gaacgatccg
      781 gcagctatta accagcaact acgtaatttt actgtggtcg ttggtgataa agatgtcgta
      841 accggcaagg atatcgccgg gctgaaaact gagcttgagc agaaaaaaat taagtttgat
      901 taccaggaat atcccggcct gaaccatgaa atggatgtct ggcggcctgc ctatgcagcc
      961 ttcgtacaga aattatttaa ataaaataag gcattaagat gaaattaatc attaccgaag
     1021 attaccagga aatgagccgt gtcgcggcac accatctgct tggttatatg tcgaagacgc
     1081 gtcgtgttaa cctggcaatt accgccggta gcacgcccaa aggcatgtat gaatacctca
     1141 ccaccctggt taaaggtaag ccctggtacg ataactgcta tttctataat ttcgatgaaa
     1201 ttccctttcg cggcaaagag ggagaaggcg taacgattac caatctgcgt aatctgtttt
     1261 tcacccctgc ggggatcaaa gaagagaata tccagaagct cactattgat aactaccgcg
     1321 agcatgatca gaaactggcg cgtgaaggcg gcctggattt ggtggtgctg ggattag
//
LOCUS       DAZPLI010000345         1364 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZPLI010000345 DAZPLI010000000
VERSION     DAZPLI010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.348"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..1259)
                     /locus_tag="ACHUU3_004481"
     CDS             complement(<1..1259)
                     /locus_tag="ACHUU3_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR2084341.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYT"
BASE COUNT          379 a          348 c          305 g          332 t
ORIGIN      
        1 gtgtaaccta ttgcgttgtt tgtattacgg tattcggcaa cgactttaat cattccctcc
       61 atcatacttg ccacttctgt ttcctgcggc gagatcatgc ggacattttt catgacctgg
      121 gattgcatca ccgtctggct accagagtct tccgggcgtt gccaggtctg gatctcctga
      181 tcgttaccgc caacagtacg ccagttggta attgcaccac tgaagatgtc acgcacctgt
      241 tgttcagtca gggaattaac cggattatcc gcattgacga tgaaaacaaa cgcttcacgg
      301 gcaaaaggcg tgtacagcaa agtaacgccc gattcctcag cgcgtttttt ctgcccaccg
      361 gaaggttgcg ccacaaaaat aatatcagca tcacctttaa caatcctgtt atacgcatcg
      421 ggggtacgag agctctccag atattccctg gtgtgaaaat cttccggtat aacgcttaaa
      481 gcataaaatg cagaggcata aattgggtac gccgccgtag caccatcgag tcgcggccag
      541 ttttgcgtaa actgaatttg cggtttacca cgcagtggtg tcagttgatt attgagttta
      601 tcgggtcgcc aggcccaggt atcaatattt tcgcgcacac cgacaccggg attgagctgt
      661 ttatataaat aaccctgata accagcgaca aaaccaagca tgagaaggat aaacaggagg
      721 gaattacggc aacgtagccc ggatgtaccg gttaaaggat gtctggttgg ccggccgtac
      781 cccagcgaaa aactgatatg cgcgccaacg ggtataatga ctatccacca gtactcacct
      841 ccgactactg agactaacaa aacgcccagc caggggagca taagatcagc caaccaaccc
      901 gatcccatct ggtgaaattg ataatctgcc agatgcagcg cgattatcca gccaaccagg
      961 attaataaac cgggtactaa aaaaagacat tgtcgccaga cggctatttc tcttttttct
     1021 ggtgcactat cgcctgccca tactcgcccc gagaaaaata gcattaatgc gcccagcgcc
     1081 agcataaaaa tgttgagcag aaggtaactt gttggcgtga taaggatatt acaaacttca
     1141 ataataccgg caagtccgac acccagcata gcaacaaccg ccgccaaaaa ttgcgtcagt
     1201 atggggatgc cgaaaaaagt cattaccagc gaggacaaaa tccattttct gttttgcatt
     1261 attctttcca ttctttttga atggtgaaat tatactcccc gagtcccctt gccccttctg
     1321 gacacttttc cgaaatgatg gcggaaaaaa acgggaccct ttgg
//
LOCUS       DAZPLI010000346         1357 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZPLI010000346 DAZPLI010000000
VERSION     DAZPLI010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.349"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..208)
                     /locus_tag="ACHUU3_004482"
     CDS             complement(<1..208)
                     /locus_tag="ACHUU3_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFR2084342.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRD"
     gene            complement(220..>1357)
                     /locus_tag="ACHUU3_004483"
     CDS             complement(220..>1357)
                     /locus_tag="ACHUU3_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR2084343.1"
                     /translation="TWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAA
                     QLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSL
                     RLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGL
                     KFHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMTIY
                     WNSRASLFCSTELNSKSQSPALGLGHEFAHAQYCLLDKENFMALLSRTDKKYENKEEA
                     RVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
BASE COUNT          324 a          402 c          249 g          382 t
ORIGIN      
        1 gatctctaat ttcattcacc accactttat atctgtatat ttcattaaga ccttccttgc
       61 taagcggaac taacgtggta acatacgatg gaagattaat tatctctatc gttttcacgc
      121 tatctattcc acacaattca ttcactcgcc ccatccccaa taccgaacca gaaaatagag
      181 acaataaaac catcaaatat ttcatcataa aaaacctcac tattctggcg tacccgttat
      241 tttcatcgtc tgcaacggtc catccacccg ataaaaaggc agtccactat gagctcctct
      301 ggtacactca cctaaagttt tcgccgcacg cgattcaatt attgtaataa cccttgcctc
      361 ttctttattc tcatatttct tatctgttcg gcttaaaaga gccatgaaat tttccttatc
      421 aagaaggcaa tactgcgcat gagcaaactc atgtcccaaa cctaatgcag gcgattgtga
      481 tttcgagttt aattcagttg aacaaaataa agatgctcta ctattccaat atatcgtcat
      541 attattcgaa ttaaatctaa cattggttcc ttcaatatac tctatattta tagtttcttc
      601 agagcttgat aaaaaatcta tagtttcttt catttgtgaa tcttgtttta aatactcaac
      661 agcctgatta aaatcacttg gatcaccgtt aacatggaat tttaaaccca aagaatctat
      721 aaaccgaaca gggtttaatg gatatttata cagattccat tcacctctca gccctatcgg
      781 atcctgggtg atataccttc cctgcaacgg gtcatagtac cggttgcggt tgtagtacag
      841 ccccgactcc tcatcatact gctgccccgg cagacggagt gactgctgga ggtgctgcgc
      901 gctggtttcg cccagcaggt ttccccattc gtcatactcc ccctgccacg ccgtttcccc
      961 ttccgggctg atgagcgcca gcggcagtcc ccggtggtcg cagtggtaaa gatgaagttt
     1021 cctctccggg atgtattccg cctccagctg cgcggccatc tgctccgccg tcagcccgca
     1081 ctgcgcaagc catgcctcac tctccgcact caccgctccc gcgcgcagtt cccgctccag
     1141 cctccccagc atcaccgaca gctccgccgg tagcgtcaca cccgtgtcct cctgcaacac
     1201 ctccgccagg ctccggtgcc gcgccttcgc ctgttcgcca ttctctgttt cgattctcag
     1261 gagcggcgtg aagctccccg gctggtatac cgtctggata cgcgtggtgc cggtctgtat
     1321 cgtcgtcagc ctgtcaccgt cccacccgta ccatgtc
//
LOCUS       DAZPLI010000347         1347 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZPLI010000347 DAZPLI010000000
VERSION     DAZPLI010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.350"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..188
                     /locus_tag="ACHUU3_004484"
     CDS             <1..188
                     /locus_tag="ACHUU3_004484"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084344.1"
                     /translation="VKSCCFFSTPPWTNFYSKKTRFVTACAQDISQQSAMGKKNDCLV
                     IFDSGNGDLKENKIFFA"
     gene            388..843
                     /gene="csgB"
                     /locus_tag="ACHUU3_004485"
     CDS             388..843
                     /gene="csgB"
                     /locus_tag="ACHUU3_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR2084345.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            884..1336
                     /gene="csgA"
                     /locus_tag="ACHUU3_004486"
     CDS             884..1336
                     /gene="csgA"
                     /locus_tag="ACHUU3_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR2084346.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          403 a          263 c          321 g          360 t
ORIGIN      
        1 atgtaaaaag ttgttgtttt ttctccacac ctccgtggac aaatttttac tccaaaaaga
       61 cgaggtttgt cacggcttgt gcgcaagaca tatcgcagca atcagcgatg ggcaagaaga
      121 atgactgtct ggtgattttt gatagcggaa acggagattt aaaagaaaac aaaatatttt
      181 ttgcgtagat aacagcgtat ttacgtggct tttaatactt tggtatgaac aaaaaaagaa
      241 aaatacaacg cgcgggtgag ttattaaaaa tatttccgca gacatacttt ccatcgtaac
      301 gcagcgttaa caaaatacag gttgcgttaa caaccaagtt gaaatgattt aatttcttaa
      361 atgtacgacc aggtccaggg tgacaacatg aaaaacaaat tgttatttat gatgttaaca
      421 atactgggtg cgcctgggat tgcagccgca gcaggttatg atttagctaa ttcagaatat
      481 aacttcgcgg taaatgaatt gagtaagtct tcatttaatc aggcagccat aattggtcaa
      541 gctgggacta ataatagtac tcagttacgg cagggaggct caaaactttt ggcggttgtt
      601 gcgcaagaag gtagtagcaa ccgggcaaag attgaccaga caggagatta taaccttgca
      661 tatattgatc aggcgggcag tgccaacgat gccagtattt cgcaaggtgc ttatggtaat
      721 actgcgatga ttatccagaa aggttctggt aataaagcaa atattacaca gtatggtact
      781 caaaaaacgg caattgtagt gcagagacag tcgcaaatgg ctattcgcgt gacacaacgt
      841 taatttccat tcgactttta aatcaatccg atgggggttt tacatgaaac ttttaaaagt
      901 agcagcaatt gcagcaatcg tattctccgg tagcgctctg gcaggtgttg ttcctcagta
      961 cggcggcggt aaccatggtg gtggcggtaa taatagcggc ccaaattctg agctgaacat
     1021 ttaccagtac ggtggcggta actctgcact tgctctgcaa actgatgccc gtaactctga
     1081 cttgactatt acccagcatg gcggcggtaa tggtgcagat gttggtcagg gctcggatga
     1141 cagctcaatc gatctgaccc aacgtggctt cggtaacagc gctactcttg atcagtggaa
     1201 cggcaaaaat tctgaaatga cggttaaaca gttcggtggt ggcaacggtg ctgcagttga
     1261 ccagactgca tctaactcct ccgtcaacgt gactcaggtt ggctttggta acaacgcgac
     1321 cgctcatcag tactaataca tcatttg
//
LOCUS       DAZPLI010000348         1345 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZPLI010000348 DAZPLI010000000
VERSION     DAZPLI010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.351"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..>1345)
                     /locus_tag="ACHUU3_004487"
     CDS             complement(<1..>1345)
                     /locus_tag="ACHUU3_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR2084347.1"
                     /translation="GAKVLPGETDLALPCPLPFILFRAYSSYRTRTPAPVGVFGPGWK
                     APFDIRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPL
                     SALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVV
                     DGFGRTLAFHRAAEGDVAGAVTGVMDGAGRRFHLVLTTQAQRAEEARKPHTASLSSPD
                     SPCPLSAPSFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGE
                     LRAVYDRSGMQVRGFTYDAEHAGRMVAHHYAGRPESCYRYDDTGRVTEQVNPEGLDYR
                     FEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDA
                     AGRRTEYRLHMASGKLTSVILPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKG"
BASE COUNT          218 a          504 c          380 g          243 t
ORIGIN      
        1 cccttctcat catactcccg gctgctgcga agcccgtccg ggtacgtcac cgacgtcacc
       61 tgccgctggc tgttatagcc ataccgtacc gtcctgccgt ccggcagtat cactgaagtc
      121 agctttccag acgccatatg cagtcggtac tccgtccgcc gtcccgccgc atccgtctgc
      181 gccttcagcc tccccgcctc atcatactcg ctgcgggtga tgctcccgtc ggcatgctcc
      241 ttcttcacca cccgcttcag gccaccctcg ccttccgtgt acagcacctc ccgccggttc
      301 aggctgtccg tgatgattac ccggctctct ccgtactcaa agcggtagtc cagcccctcc
      361 gggttgacct gctccgtcac ccgaccggta tcatcatacc ggtagcagct ctccggcctc
      421 cccgcataat ggtgcgccac catccgcccg gcgtgctccg catcataagt aaacccgcgc
      481 acctgcatcc cgctgcggtc atacaccgcc cgcagttcac caccggccgt gtacgtgtag
      541 cgcgccagcg gcgcagtcgg ctgttcatcc gggtatgccg ggtcgtgtgt cagccatacc
      601 gcctccagcc ggataccgtt atcggcaccg tactctgtac cggcgggcag cgtgtcgggg
      661 aaggaaggag ctgagagagg gcagggactg tcaggggaag ataacgaagc ggtgtgcggt
      721 ttacgggcct cttccgcccg ctgcgcctgt gtggtcagca ccaggtgaaa acggcggccc
      781 gcgccgtcca tcacccccgt caccgcgccc gccacgtcac cctccgctgc ccggtgaaag
      841 gccagcgtcc gcccgaagcc gtccaccacg cccgtcagca cccggtatgc gggcggctcc
      901 ggcggcagca cctcgtccgc ccccggcacc cgctccggcc agctgagtat ccaccacggc
      961 ccctgaaggc tgtttgtcgc caggtacaca tgcggactca ggcgaacatc ttccggcagc
     1021 acctgccaga gcgcgcttag cggctgcgaa ctgtgctgcg ccgctactcc gccccgcgcc
     1081 agccagagtg actcgctgcg gctgtagctt atctcgccgg gaaacagcgg ctcaaagtga
     1141 atgctcctgc cgccgctgtc gttgagtatc aggccttcat cgcgtatctg taagcggata
     1201 tcgaagggcg ctttccagcc gggaccaaac acccccaccg gcgcgggtgt tctggtccgg
     1261 tagctgctgt aggcgcggaa aagaataaac ggcagcgggc agggcagcgc aaggtccgtc
     1321 tcgcccggca gcaccttcgc accca
//
LOCUS       DAZPLI010000349         1341 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZPLI010000349 DAZPLI010000000
VERSION     DAZPLI010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.352"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..184
                     /locus_tag="ACHUU3_004488"
     CDS             <1..184
                     /locus_tag="ACHUU3_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084348.1"
                     /translation="TISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQ
                     GKRRCQQQFTSQEVQN"
     gene            194..>1341
                     /gene="yicI"
                     /locus_tag="ACHUU3_004489"
     CDS             194..>1341
                     /gene="yicI"
                     /locus_tag="ACHUU3_004489"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR2084349.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          335 a          318 c          351 g          337 t
ORIGIN      
        1 cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag
       61 cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga
      121 acagctggct cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa
      181 ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt
      241 gattcacccg cttcaggtgt tcgaggttga acagcaggat aatgaaatgg tggtctatgc
      301 tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg
      361 ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct
      421 gaataacggt cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa
      481 cacagaacgt tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga
      541 gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa
      601 taatggctac gtgcaggaca cgaataatca acgcaattat atgtttgagc ggcttgatct
      661 tggcgttggc gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg
      721 ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa
      781 tatcccgttc tacatgacta accgtggtta tggggtactg gtcaatcatc cccagtgtgt
      841 ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata
      901 tctcgaatac tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg
      961 ctttactggt cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc
     1021 atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg
     1081 caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg
     1141 cgattttgag tgggacccgc tgactttccc tgacccggaa gggatgatcc gccgcctgaa
     1201 agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcggtcaaa aatcccccgt
     1261 ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt cgctatggca
     1321 gtgggataaa tggcagccag g
//
LOCUS       DAZPLI010000350         1330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZPLI010000350 DAZPLI010000000
VERSION     DAZPLI010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.353"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..>1330)
                     /locus_tag="ACHUU3_004490"
     CDS             complement(<1..>1330)
                     /locus_tag="ACHUU3_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR2084350.1"
                     /translation="PVNPVLGAKVLPGETDLALPCPLPFILFRAYSSYRTRTPAPVGV
                     FGPGWKAPFDIRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQ
                     HSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPEPPAYRVLTVVVDGFGRS
                     LTFHRAAEGDVAGAVTGVTDGAGRRFHMALSTQAQRAEASRKQRASSLSSPASPRSVS
                     SSQVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGELRAVY
                     DRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRFEYGE
                     SRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRRT
                     EYRLHMASGKLTSVVLPDGRTVRYGYNNQLQLTSVTYPDGLRSSRKYDRQG"
BASE COUNT          230 a          482 c          376 g          242 t
ORIGIN      
        1 ccctgacggt catatttccg gctgctgcgt aaaccatccg gatatgtcac tgacgtcagt
       61 tgcaactggt tgttgtaacc ataccgtacc gtcctgccgt ccggcagtac cactgaagtc
      121 agctttccag acgccatatg cagccggtac tccgtccgcc gccccgccgc atccgtctgc
      181 gccttcagcc tccccgcctc atcatactcg ctgcgggtga tgctcccgtc ggcatgctcc
      241 ttcttcacca cccgcttcag gccaccctcg ccttccgtgt acagcacctc ccgccggttc
      301 aggctgtccg tgatgattac ccggctctct ccgtactcaa agcggtagtc cagcccctcc
      361 gggttgacct gctccgtcac ccgaccggta tcatcatacc ggtagcggct ctccggcctc
      421 cccgcataat ggtgcgccac catccgcccg gcgtgctccg catcataagt aaacccgcga
      481 acctgcgtcc cgctgcggtc atacaccgcc cgcagttcac caccggccgt gtacgtgtag
      541 cgcgccagcg gcgcagtcgg ctgttcatcc gggtatgccg ggtcgtgtgt cagccatacc
      601 gcctccagcc ggataccgtt atcggcaccg tactctgtac cggcgggcag cgtgtcgggg
      661 aaaacctgag aagaggaaac agaacgggga ctggccgggg aagataagga agaggcgcgc
      721 tgtttacggg acgcttccgc ccgctgcgcc tgcgtgctca gcgccatgtg aaaacggcgg
      781 cccgcgccgt ccgtcacccc cgtcaccgcg cccgccacgt caccctccgc cgcccggtgg
      841 aaagtcagtg agcgcccgaa gccatccacc acgaccgtca gtacccggta tgcgggcggc
      901 tccggccagc tgagtatcca ccacggcccc tgaaggctgt ttgtcgccag gtacacatgc
      961 ggactcaggc gaacatcttc cggcagcacc tgccagagcg cgcttagcgg ctgcgaactg
     1021 tgctgcgccg ccactccgcc ccgcgccagc cagagtgact cgctgcggct gtagcttatc
     1081 tcgccgggaa acagcggctc aaagtgaatg ctcctgccgc cgctgtcgtt gagtatcagg
     1141 ccttcatcgc gtatctgtaa gcggatatcg aagggcgctt tccagccggg accaaacacc
     1201 cccaccggcg cgggtgttct ggtccggtag ctgctgtagg cacggaaaag aataaacggc
     1261 agcgggcagg gcagcgcaag gtccgtctcg cccggaagca ccttcgcacc cagcaccggg
     1321 ttcaccgggt
//
LOCUS       DAZPLI010000351         1318 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZPLI010000351 DAZPLI010000000
VERSION     DAZPLI010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.354"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..346)
                     /locus_tag="ACHUU3_004491"
     CDS             complement(<1..346)
                     /locus_tag="ACHUU3_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084351.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERA"
     gene            complement(349..1059)
                     /locus_tag="ACHUU3_004492"
     CDS             complement(349..1059)
                     /locus_tag="ACHUU3_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084352.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(1075..>1318)
                     /locus_tag="ACHUU3_004493"
     CDS             complement(1075..>1318)
                     /locus_tag="ACHUU3_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR2084353.1"
                     /translation="RYYATQMNADKNIKLLAINGIAPTAENIRNGKYPYIVDAFMVTR
                     ENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT          349 a          317 c          293 g          359 t
ORIGIN      
        1 ttgcccgttc atccgcacgc cagttttctt tgttggcaag gttgatacgg cgcagagaaa
       61 aaactacgtg aatattgtca aaatgcccct gtgcaacaaa ttccagtaaa cgcggcagaa
      121 aatagcggac ctcatcgcta ttttgcggat caggccctgg gctactattc caggcattga
      181 ttaaggaata cgggatcgct cgtaacgatg tactccgtaa tgccttttgt tcctgctgac
      241 tcaggcaata ttcacaacaa accacaaatt tctgcggcac ccgataacga cgaaaggtgt
      301 gataaatctg ctcaattaat gcctgcatgg cggggctggg ttgcatagtt accccctcgc
      361 ccgctgatcg cgtgtaaacg gttgatagac aaaacgagca ttcgccgcgt tcaggcgaat
      421 aagggcgcta cgcgaaaggg aaaagctggg gcgaatgcaa ctattgcagt ctcgacagct
      481 ttcctctgcg caattttcca ctgagtaatg acgcgtgtag agtttcagtt cggcctgctt
      541 atcgcgaaat ccgcgtaaac tgttattgtt atgaaatttc caggcatctt taaagacctg
      601 aaagatatag acatcctcaa tccaggagtc acatttttta agcgggcgct ttgctattat
      661 cacccgctca tccggattta gcatcgccgt aaattgtacg gtgacgtcat ttgtcgtgtt
      721 caggtcacat tgaacatctg gtacatcact gatatagggg tcaaatccaa aatcagcacc
      781 acaggcacaa agacggctaa tggtggcggt atcgagggaa aatcccggcc ataccttgct
      841 ggaggttaag ccaatattta atgtgacatc atatcgaagc aatccctcaa gaccgtcagc
      901 accgacgaac ttttcccctt catccaaggc ctggttaata agttgctgaa caatgccttc
      961 atcccagtat gccgatttgc agttggcaat cgtaatatcc acgcctgtga agggatcagt
     1021 accggcaagg cttgcaaata atgtattaac taattccatt tttattatcc agctttaagg
     1081 tagcgtctta tacatcggga cataacctac atcttccacc aaactctgtc cctgcggcgt
     1141 taaaaaccac tccaacaatt tttgtgtctc tgacgtcgta ttttcccgcg ttaccataaa
     1201 ggcatcgacg atatagggat atttgccgtt gcgaatgttt tcggcagtcg gtgcaatacc
     1261 gttaatcgct agcagtttta tatttttatc ggcgttcatt tgtgttgcgt aataacgg
//
LOCUS       DAZPLI010000352         1305 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZPLI010000352 DAZPLI010000000
VERSION     DAZPLI010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.355"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            27..518
                     /locus_tag="ACHUU3_004494"
     CDS             27..518
                     /locus_tag="ACHUU3_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR2084354.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKRENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            853..>1305
                     /gene="ygeH"
                     /locus_tag="ACHUU3_004495"
     CDS             853..>1305
                     /gene="ygeH"
                     /locus_tag="ACHUU3_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFR2084355.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKID"
BASE COUNT          479 a          204 c          214 g          408 t
ORIGIN      
        1 cacgagacac atcacagagg aatattatga gcacagaaac aattgaaata ttcaataata
       61 gtgatgaatg ggcaaatcaa ctaaaacacg cattatcgaa aagagaaaat ctggcattac
      121 tacatggttt aactcctgat atccttgata gaatatatgc atatgcattc gactaccatg
      181 aaaagggtaa tataacggac gcagaaattt attataaatt tttgtgcatt tatgcgttcg
      241 aaaatcatga gtatctaaaa gattttgcat cagtatgtca gccaaaaaag aaatatcaac
      301 aagcatatga cctttacaaa ctaagttaca attactcccc gtatgatgac tattcagtta
      361 tttatcgtat gggtcaatgt cagattgggg ctaaaaatat cgataacgca atgcaatgtt
      421 tctatcacat tattaacaat tgtgaggatg atagtgttaa gagtaaagcg caggcatata
      481 ttgaactctt aaacgataat tcagaagata atggctaagc attacaatca tttggttaaa
      541 tttgctggtt atagtgcagt taactactat aaccagcacc tcttatgttt tcttaaatca
      601 gcatcttaaa acagttaaga gcatactata aatcattatt tttcaatgcg ttagagaaca
      661 atctacttta taaaaattga ataaggctat ttcagattac atcactgaat tcctaagata
      721 aattagtgaa cacaaccata gatatgtcga atgtattttc ctgtagcatt aatgacacat
      781 aaatatgaat agccataatt tctattgctt aagcaggatg caagaaacca attttttcat
      841 agaggttaac taatggactt agaaaataaa ttctcatatc attttcttga gggattaacg
      901 ctcacggaag atggaattct tactcaagga aatgagcaag tttatattcc acagaaagag
      961 ttaggtgtat taatagtatt acttgaatcc gctggtcatg ttgtactgaa agatatgatc
     1021 atcgaatcag tatggaaaaa tattattgtt agtgacgagt ccctgacaag atgtatctat
     1081 tctttgcgct gcatttttga aaaaattggc tatgatcgtt gcatagaaac aatctaccgg
     1141 aaaggttatc gtttcagcgg gcaggttttc aaaactaaaa ttaatgaaga taatacttca
     1201 gactattcca tagctatatt ccctttcact acttcattga atacactgga tccattaata
     1261 cttaatcagg aattagtgca aatcatttca aataaaaaaa tcgat
//
LOCUS       DAZPLI010000353         1286 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZPLI010000353 DAZPLI010000000
VERSION     DAZPLI010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.356"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            1..1196
                     /gene="bluF"
                     /locus_tag="ACHUU3_004496"
                     /pseudo
     CDS             1..1196
                     /gene="bluF"
                     /locus_tag="ACHUU3_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
BASE COUNT          373 a          269 c          276 g          368 t
ORIGIN      
        1 atgcttacca cccttattta tcgtagccat atacgtgacg acgaacctgt caaaaaaatc
       61 gaagaaatgg tttcgatagc aaatcgcagg aacatgcagt ctgacgtaac agggatctta
      121 ctgtttaatg gttctcattt tttccagctt ctggaaggtc cggaagaaca ggttaaaatg
      181 atatatcggg ctatatgcca ggatccacgg cactataata ttgttgagct gctgtgcgat
      241 tacgcgcctg ctcgccgttt tggcaaagcg ggaatggaat tatttgattt gcgcctgcac
      301 gagcgagatg acgttttaca ggccgtattc gacaaaggca catcaaaatt tcagctaact
      361 tatgatgaca gagcgctaca attttttcgt acttttgtcc ttgcaaccga acaatcaacc
      421 tatttcgaga tccctgccga agactcctgg ctttttatcg ctgacggatc tgataaagaa
      481 cttgattcct gtaccctttc accaactata aacgaccact ttgcctttca tcccttatcg
      541 cggcggataa tcgcttttga agccattgtg caaaaaaatg aagcccatca gccatagcgg
      601 ttgggcagcg taaagacggg gaaatctaca cagcggatct caaaagtaag gcgcttgcat
      661 tcgcgatggc acacgcactt gagctcggtg ataaaatgat ttcaatcaat ctattaccta
      721 tgaccctggt taacgaacct gacgcagtct cttttttact tgatgaaata aaggccaatg
      781 ctctggtgcc tgaacaaatc atcgttgaat ttactgaaag tgaagttata tctcggtttg
      841 atgagtttgc agaagcgatt aaatcgctta aggctgccgg tatcagtgta gcaattgatc
      901 attttggcgc aggttttgcc ggtttgttac tcctgtcacg cttccagcct gacagaatta
      961 aaatcagtca ggaattgatt accaatgttc ataaaagcgg gccacggcag gcaattattc
     1021 aagcgatcat aaaatgctgt acatcacttg aaattcaagt cagtgctatg ggcgtggcaa
     1081 caccagaaga gtggatgtgg cttgaatccg caggaattga gatgtttcag ggagatctgt
     1141 ttgctaaagc taaattgaat ggtatccctt ccatagcgtg gccagagaaa aaataatttt
     1201 cagcacattc tttcacatga tttcagtaaa tcatcgtgaa aatgaatacg ttatgtttaa
     1261 atttaatcca cattaaatat aatgtt
//
LOCUS       DAZPLI010000354         1285 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZPLI010000354 DAZPLI010000000
VERSION     DAZPLI010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.357"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..1240)
                     /locus_tag="ACHUU3_004497"
     CDS             complement(<1..1240)
                     /locus_tag="ACHUU3_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR2084356.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNIINNVFKNVS"
BASE COUNT          415 a          242 c          186 g          442 t
ORIGIN      
        1 acgaaacatt tttgaataca ttatttatta tattgctatt tgttatttta acatcaccta
       61 aactgccgtt ttcaaaacaa cattttttca atatgctttc attcacaaca gtgtgtgata
      121 agtagagatt acttatgttt tgctttatta atgccacgtc attaaaatga ctatatttaa
      181 tgaaaaaacc atcattgagt agtgttttaa gattgactaa attacttgca tcttcacgta
      241 ccatcgtggc aactggaaca ggcagagaca tgaaaacttc ttcattcacc atcaagtgaa
      301 tcatgcattg cgttcccttt gtcatatcca cacatacgct acaaatatcg tggtaatgcg
      361 aagagcgcat ttcaggaaac agattaaaca cggtatcttg aaaggcacta aatcgtgtta
      421 ataaatcctg ataatgaaca ggttttgttt caaaattatc atcagcaaat ccaagcacta
      481 ttgtatgctc tacatctaac gcagtttcaa atggctcttc ttctgtaaaa tcattattta
      541 aaacctcacc tgacgtatta tcactgtcat aaaaagtgtt catcaatgcg atattatttg
      601 ttacagcgtc tttttcgata ttctcactaa ttgcatatcg cctgctataa tcataaaaat
      661 gaataaacgc gccctttata aaggatatat attgttcttt gttaaagtca atacggctgt
      721 ataatttttc aaaaatcaca ttcgcaattt tactaccaat ggtatgcctg tataataatg
      781 ggtgcgattc aatcgtaaaa tgcttgtcta attgatcttg taatttattc tcatagtcca
      841 gagggaaaag aaactgttgt ctttcaagtt tcccattcga tacattacgt attccaatag
      901 agattctttc attacccgcc tttatttcaa cttcgttatc ttctgattta cccagatatt
      961 ttttaacatc agtctcgaaa gcattactaa aaaaagtatt aaattggtaa ttcgctgatg
     1021 gcatattttc cgcgagcaac acatcatttt ttatgagatc actgtcagca tttattgcta
     1081 atatcagatg caagtagatg gtatcccatg tagacaactc acccttacga ctggaattta
     1141 atatcgcgaa aaattccctc agtgaatcca ggaaattatc actttgattt agttgtgtgc
     1201 ctggaaatga tgatgcttga tcagatatat tgtttatcat attaaaaatc acccaagaaa
     1261 aacgataatc caccgaactc ctcgt
//
LOCUS       DAZPLI010000355         1237 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZPLI010000355 DAZPLI010000000
VERSION     DAZPLI010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.358"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            131..808
                     /locus_tag="ACHUU3_004498"
     CDS             131..808
                     /locus_tag="ACHUU3_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR2084357.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            880..1164
                     /gene="ppnP"
                     /locus_tag="ACHUU3_004499"
     CDS             880..1164
                     /gene="ppnP"
                     /locus_tag="ACHUU3_004499"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR2084358.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          309 a          273 c          313 g          342 t
ORIGIN      
        1 gaatctttac attatgccgt gcacgtctgc tgctacgctt tttgtcattt gtagcacaag
       61 taagtgtcag cagtggtgct tcacacttgc ccggtaatta acgacgaaag aaaagtaagg
      121 tggatgaaca atgagtgcgt cgttggcgat cctgaccatc ggcattgtac ctatgcagga
      181 ggttttgccg ctcctgacgg aatacattga cgaagataat atttcccatc atagcctgct
      241 ggggaagtta agtcgtgaag aagtgatggc ggagtacgcg ccagaagcag gcgaagacac
      301 cattctcaca ttattaaatg acaaccagct ggcccatgtt tcgcgtcgta aagtggagcg
      361 agacctgcaa ggtgtggttg aagtgctcga taatcagggt tatgacgtca ttttattaat
      421 gagtacagca aacattagta gtatgactgc gcgtaatacg atctttcttg agccgtcgcg
      481 aatattgcct ccactggttt cctctattgt tgaagatcat caggtggggg ttatcgttcc
      541 ggttgaggag atgctgaccg ttcaggcgca aaaatggcaa attttgcaga aatcgccggt
      601 attttcattg ggtaacccca ttcatgattc agaacaaaaa atcattgatg ccgggaaaga
      661 attactggca aaaggggctg atgtcatcat gctggattgt ttaggatttc accaacgtca
      721 tcgcgattta ctgcaaaaac agctcgatgt tcctgtcttg ctgtctaacg tattgattgc
      781 acggctggct gcggaattac tggtgtaatt ttgcgtgaca gccagcgcct ctggccccta
      841 tagtgaagta gatgttcaac taccagacag ggccagttta tgcttcaaag taatgagtac
      901 ttttccggca aagtgaaatc aatcggcttt tccagcagca gcactggtcg cgccagcgtg
      961 ggtgttatgg ttgaaggcga atacaccttc agcaccgctg agccggaaga gatgacggta
     1021 atcagtggcg cgctgaatgt gttactgcct gacgcgaccg actggcaggt gtatgaagcc
     1081 ggttcggtgt ttaatgttcc cggtcacagt gagtttcatc tgcaagttgc cgaacccacc
     1141 tcttatctgt gccgctatct gtaattcctc gccttcccct tgaacgggag ggcatttttc
     1201 tgaaatatcc tctctttaac ccataataat atttcct
//
LOCUS       DAZPLI010000356         1235 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZPLI010000356 DAZPLI010000000
VERSION     DAZPLI010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.359"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..267
                     /gene="ykfM"
                     /locus_tag="ACHUU3_004500"
     CDS             <1..267
                     /gene="ykfM"
                     /locus_tag="ACHUU3_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkfM"
                     /protein_id="HFR2084359.1"
                     /translation="LTIIKLFLIKKTKVVNFATDITIQLTLIFLLIAIVIAPLIAPFV
                     TGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(404..1189)
                     /locus_tag="ACHUU3_004501"
     CDS             complement(404..1189)
                     /locus_tag="ACHUU3_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084360.1"
                     /translation="MNLRRVCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          356 a          262 c          214 g          403 t
ORIGIN      
        1 cttacgataa taaaattatt cttaatcaaa aaaacgaagg tagtcaattt cgccacagac
       61 ataacaatac agctaactct tatattttta ctaatagcta tcgtaatagc acctttaata
      121 gctccttttg tcacaggata tgtcaatgct aattatcacc cctgcggtaa taatacggga
      181 attttcccag gagcaatcta tattaaaaat ggcatgaaat gtaataatga atatatttct
      241 cgcaaggaag attcagcagt taaataatta aacattctaa aacaaacagt ttttaactcc
      301 ggattccatg tcctttcttg cactgtcttt gtcaagaaaa atttcttatg caaagaattt
      361 tgtattattt ccgccagaaa ttcctggcgg cagatataat ttattattga tagcggttaa
      421 ccaaatcttt taccatcgct ttataggtct tttgcttaag ttgcatgatt tgctgctctg
      481 tcatatcagt cgtactggct tcattcttgt cgggcaatgg tggaagcact ttatattcat
      541 aattcaccgg cgaccaggca gcccactcac ctgtagctac atccaccagc gtgaatcgta
      601 ccagatagcg cagtgacatc gtgtcagtca atttttgatt tttataatct gaccaaacta
      661 ttgatttcaa tccagagtca tattttcctg tctgcaacgt atcctggaaa acaataatcg
      721 ctttctgatg cccttttgca gccacaaaac gcagtgcctg catccagttc atattctcag
      781 cactggcaac atcctcgttc tcatttttat ctttgttttt gttacaagtc agaggctttt
      841 gcctgtccgg gataccagag aatgtggaaa cagtataata ttttcgcatc tcctcctgca
      901 taatggtttc cggtgcgcgg ttgccggatt gaacaagtat tacaggagaa ttaaggggaa
      961 cgcggaactc atctccatca agcgcagctt gaatatcctc ttcggatacg gccagagtgg
     1021 tttcattacc gaataaatct ttgtctgtca gttgctgggg agtgttttta ttaatattac
     1081 tttgtgtgac ttgagctttt ttcttctttt catcaataga agattcagag gcacatcctg
     1141 caagcagcga gaataataca catatacaac aaacccttct taaattcata atcggacact
     1201 ccctcgcctg tcataatcat ggtaatgttt agcta
//
LOCUS       DAZPLI010000357         1234 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZPLI010000357 DAZPLI010000000
VERSION     DAZPLI010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.360"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..761)
                     /locus_tag="ACHUU3_004502"
     CDS             complement(<1..761)
                     /locus_tag="ACHUU3_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR2084361.1"
                     /translation="MLNVAIENQNGWNYSAPAPYKTGAGRGNPNVTRAHSRAEAVFLC
                     VMHSSIQIMVGCAGQSQDWPGSRVTGISTPVRLTTLMVVENLGGELINLSLEDAIMAT
                     IPALSHPDVTIENGRAVTTSIAIAEFFGKRHDNVLRAIANTECSPEFNALNFEDVTYT
                     DAKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPTDKI
                     IPGDGRTLVVHFDERGNIRFTETVPDGARVCTMET"
     gene            1019..>1234
                     /locus_tag="ACHUU3_004503"
     CDS             1019..>1234
                     /locus_tag="ACHUU3_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084362.1"
                     /translation="MAWLSLKRMVNLTTRCPQRGRNNSAILTAASFRVVCLLLTTSGE
                     VVSFLSRITKKEIYMTKEEFVSYIFDKT"
BASE COUNT          307 a          299 c          310 g          318 t
ORIGIN      
        1 aaagtctcca tggtacagac ccgtgcgcca tcaggaacgg tttcggtgaa tctgatgttg
       61 ccgcgttcgt cgaagtgaac aaccagggtg cggccatcgc ctggaattat tttgtcagtg
      121 ggtggggtgt tattctggcg cagttcggcc tccatgcgat cgaactcagc aatgtaggct
      181 tctttaaatg cagcggcttt tttgccagtg aatcccatca ccaggaaaac gaagccgttt
      241 ttggtgattt ggtacatcgg gcgtttttcg cctttggcgt cagtgtaggt gacgtcctca
      301 aaattgaggg cgttaaattc aggtgagcat tcagtatttg cgatggcacg taacacattg
      361 tcgtgacgct tgccaaagaa ctctgcgatc gcaatagacg tagtgacagc gcgaccattt
      421 tcgatggtta cgtcagggtg agaaagggca gggatagtag ccatgatggc atcctcgagt
      481 gataagttaa ttaactcacc accgaggttt tccacgacca taagggtggt gagacgtaca
      541 ggggtggaaa taccggtcac tcgagaaccc ggccagtctt gcgactgccc tgcacatccc
      601 accataattt gaatggagct gtgcattacg cataaaaaaa ccgcttcagc gcggctatgc
      661 gctcgagtaa cgttcgggtt tccacgcccg gcacccgttt tataaggtgc aggtgcacta
      721 taattccacc cgttctggtt ttcaatagct acattcaaca ttttctcttt cctttcatca
      781 ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag gtgacgttaa ccagttaaca
      841 attaacgccg gatacaggga aacccataac cacccgatac gggggaacta atcgcttttt
      901 aactgtgccg cgtgcgctga gccgcattca ccgcatcaca aaattcactt taaaaagggc
      961 ggacatcagc cagcaatgaa actgatgccg ccaaaggtac caatcaacat ggagtgttgt
     1021 ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc cggtgtcctc aacggggaag
     1081 gaataactcc gccatactta ccgccgcgtc atttcgcgtt gtgtgcctgc ttttaaccac
     1141 gtcaggcgag gtggtatcct tcttatcccg aataaccaag aaggaaatct atatgactaa
     1201 agaagaattt gtctcttata tttttgataa aacg
//
LOCUS       DAZPLI010000358         1221 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZPLI010000358 DAZPLI010000000
VERSION     DAZPLI010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.361"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            190..708
                     /locus_tag="ACHUU3_004504"
     CDS             190..708
                     /locus_tag="ACHUU3_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR2084363.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            918..>1221
                     /locus_tag="ACHUU3_004505"
     CDS             918..>1221
                     /locus_tag="ACHUU3_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR2084364.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
BASE COUNT          297 a          307 c          315 g          302 t
ORIGIN      
        1 ttattgctga ataagagatt taatgatttt aaaaatagtt tttacgacaa ccgtaaattc
       61 tagctcggtt tgtatgtaaa aaatactgaa aaatgatgcg gttccgtgaa gtgagctgca
      121 tctgcctgtc tgggattaac ggcaggtcat tgtaagtagt aattaaccac gttaacaagg
      181 agtttacaaa tgccaacacc gtgttatatc tccatcactg gtcagaccca gggaaatatt
      241 actgcaggtg catttaccgc tgattctgtc ggcaacatct atgtgcaggg gcacgaagac
      301 gagatgctgg tgcaggaatt cctgcataac gttactgtcc cgaccactcc tcagtccggt
      361 cagcctgcgg gtcagcgtgc tcacaagcct ttcattttta ccgtggcgct gaacaaagcg
      421 gttccgctga tgtacaacgc actggcctcc ggcgaaatgc tgccaaccac tgaacttcac
      481 tggtggcgta cctctgtgga gggcaaacag gagcattatt tcactacccg cctgactgat
      541 tcgacgattg tcgatatgaa actgcatatg ccgcattgcc aggacccggc gaaacgcgag
      601 ttcacccagt tgctcgaggt ttcactggcc taccgcaaaa ttgaatggga acacgtcaaa
      661 tctggcacct ccggtgccga cgactggcgc gcaccactgg aagcataagt taagccaaca
      721 gcatccggct atcaggccgg atgctgtttt tgtattacgc cgtagaaaga aaggttgtca
      781 gtaaggggtt acgttcacgg gagatggcgg atgccggaaa cttcggcggt agcgtgcctg
      841 aacgcaagcg taacactgta ctgtcacagc cgctttaaca gcgaactgtc tgtttttcct
      901 taaccccgga ggttgctatg tcaaccggat tacgtttcac gctggaagtg gacggcctgc
      961 caccggatgc ttttgcggtg gtctcctttc atctgaacca gtcactctct tcgctctttt
     1021 ccctcgagct ctctctggtc agccagcagt ttctctccct tgaatttccg caggtactgg
     1081 acaaaatggc ctacctgacg atatggcagg gcgatgacgt acagcgccgg gtgaaaggtg
     1141 tggtgacctg gtttgaactg ggggagaacg acaaaaacca gatgctgtac agcatgaagg
     1201 tatgcccacc gctgtggcgc a
//
LOCUS       DAZPLI010000359         1218 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZPLI010000359 DAZPLI010000000
VERSION     DAZPLI010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.362"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            248..>1218
                     /gene="cadB"
                     /locus_tag="ACHUU3_004506"
     CDS             248..>1218
                     /gene="cadB"
                     /locus_tag="ACHUU3_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR2084365.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT          272 a          262 c          295 g          389 t
ORIGIN      
        1 ccagagaatg tcatgcaatc cattgtaaac attgaatgtt tatcttttca tgatatcaac
       61 ttgcgatcct gatgtgttaa taaaaaacct caagttctca cttacagaaa cttttgtgtt
      121 atttcaccta atctttagga ttaatccttt tttcgtgagt aatcttatcg ccagtttggt
      181 ctggtcagga aatagttata catcatgacc cggactccaa attcaaaaat gaaattagga
      241 gaagagcatg agttctgcca agaagatcgg gctatttgcc tgtaccggtg ttgttgccgg
      301 taatatgatg gggagcggta ttgcattatt acctgcgaac ctagcaagta tcggtggtat
      361 tgctatctgg ggttggatta tctctattat tggtgcaatg tcgctggcgt atgtatatgc
      421 ccgactggca acaaaaaacc cgcaacaagg tggcccaatt gcttatgccg gagaaatttc
      481 tcctgcattt ggttttcaga caggtgttct ttattaccat gctaactgga ttggtaacct
      541 agcgattggt attaccgctg tatcttatct ttccaccttc ttcccagtat taaatgatcc
      601 tgttccggcg ggtatcgcct gtattgctat cgtctgggta tttacctttg taaatatgct
      661 cggcggtacc tgggtaagcc gtttaaccac tattggtctg gtgctggttc ttattcctgt
      721 ggtgatgact gctattgttg gctggcattg gtttgatgcg gcaacttatg cagctaactg
      781 gaatactgcg gataccactg atggtcatgc gatcattaaa agtattctgc tctacctgtg
      841 ggccttcgtg ggtgttgagt ccgcagcagt aagtactggt atggttaaaa acccgaaacg
      901 taccgttccg ctggcaacca tgctgggtac tggtttagca ggtattgttt acatcgctgc
      961 gactcaggtg ctttccggta tgtatccgtc ttctgtaatg gcggcttccg gtgctccgtt
     1021 tgcaatcagt gcttcaacta tcctcggtaa ctgggctgcg ccgctggttt ctgcattcac
     1081 cgcctttgcg tgcctgactt ctctgggctc ctggatgatg ttggtaggcc aggcaggtgt
     1141 acgtgccgct aacgacggta acttcccgaa agtttatggt gaagtcgaca gcaacggtat
     1201 tccgaaaaaa ggtctgct
//
LOCUS       DAZPLI010000360         1211 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZPLI010000360 DAZPLI010000000
VERSION     DAZPLI010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.363"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..680)
                     /locus_tag="ACHUU3_004507"
     CDS             complement(<1..680)
                     /locus_tag="ACHUU3_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR2084366.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
     gene            complement(996..>1211)
                     /locus_tag="ACHUU3_004508"
     CDS             complement(996..>1211)
                     /locus_tag="ACHUU3_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR2084367.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          355 a          211 c          252 g          393 t
ORIGIN      
        1 aacaaagttt gcattattct gggagatagt tgattttctc taagattgac aacgctatca
       61 gaactgaggc caagtatgct ttccggaatt gccgtgatcc ggttctgatt aagccagaat
      121 attcttataa tatgatcatc tctttctgga aaatctggta ttacttccaa agcatttctg
      181 gctgcatcaa gtaattccaa tgacattggt aacctcggaa gtacagacag gtgattgtca
      241 cttacattaa tatattctag tgacgcaggt aattcaggaa gtgcgaacaa atggttatca
      301 ctcacattta tatattccag cgacactggt aattcaggaa gtgcagataa ttgattactg
      361 cttgcattca gctctttcaa tgcccttggt agctcgggga gcattgatag ttggttattg
      421 cttacattaa tttcatcaag attgtcaggc aatcgtggta gagatctgag acctaaacaa
      481 gataagtcca acgatgtttc actgttctcc agacataatt ggagccgggt aaaagcagtt
      541 tccctgtttt ctccggaaat gctgttttta gtccattcaa cccattcgga gagataatta
      601 ttgtgaacat tgtcgattga tgtagttctg taaaaagaga cgtttccagt ggataggggg
      661 ggattattta caggaagcat aataacctcg cagaaggata ttctgataaa atgtgatact
      721 gggaaataat aagctgaaat aaaatgtttc aggattgaaa tttattgttt tcattttaag
      781 gaagagatgt tgatttaact gtaaaaaaac gatactattt tcttagatgg taaatgtctc
      841 gcctgtgcta tattttgttt ttcggttagt taaatatcgt acgttcatat ttgaacgttc
      901 tgccggaatg cattatcaat agaggtaaag tcgcaacccc aaatcgtaaa ggaaaccgta
      961 gcacgtctta agcaagaacg tgctgcggtt ggatgctatt ttttccctga agcggaaaac
     1021 attactacag taccttgaac cttggtttta acattctcga aatgctctga gagtatatgt
     1081 gttaagcctt cttcggaatc ttttgtgttt gaaaagatgc ctttctgatt gtaaatgcgc
     1141 atcagttttt gaccgaagct attgtgcaca actccatcgc caagaattgt ggctccgtat
     1201 agagttccat c
//
LOCUS       DAZPLI010000361         1202 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZPLI010000361 DAZPLI010000000
VERSION     DAZPLI010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.364"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            21..1103
                     /locus_tag="ACHUU3_004509"
     CDS             21..1103
                     /locus_tag="ACHUU3_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFR2084368.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
BASE COUNT          338 a          260 c          271 g          333 t
ORIGIN      
        1 gttcaaaaaa agggctcacg atgaaaaaat taacagtggc aatttctgct gtagctgcat
       61 cagtactgat ggcgatgtct gctcaggcag ctgaaattta taataaagac agtaacaagc
      121 tggatctgta cgggaaagtt aatgctaagc actacttctc ctctaatgat gcagatgatg
      181 gtgatactac ttatgcccgt cttggcttca aaggtgaaac ccaaatcaac gatcaactga
      241 ctggtttcgg tcagtgggaa tatgaattca aaggcaaccg cgctgaatct caaggttcct
      301 ccaaagataa aacccgtctt gccttcgctg gcctgaaatt cggtgactac ggctccatcg
      361 attacggccg taactacggt gtagcatacg acatcggtgc gtggactgac gtcctgccag
      421 aattcggtgg tgacacttgg actcaaacag atgtgttcat gactggtcgc accactggtg
      481 ttgcaactta tcgtaacaac gacttctttg gtctggttga tggtctgaac tttgctgctc
      541 agtatcaggg taaaaatgac cgcactgacg taactgaagc taatggtgat ggtttcggtt
      601 tctccactac ttatgagtat gaaggattcg gtgtaggtgc aacctatgct aaatctgacc
      661 gcactaataa tcaggttatc tacggtaaca acagcctgaa tgcatctggt caaaatgctg
      721 aagtatgggc agctggtctg aaatatgatg cgaacaacat ctatctggct accacctatt
      781 ctgaaaccca gaacatgact gtttttggta ataaccatat tgccaacaaa gcacaaaact
      841 tcgaagtagt tgcacaatat cagttcgact tcggtctgcg tccgtccgtt gcttacctgc
      901 aatctaaagg aaaagacttg ggtgcgtggg gtgatcagga cctggttgaa tatattgatg
      961 taggtgcaac ctattacttc aacaaaaata tgtccacttt tgttgattac aaaatcaacc
     1021 tgattgataa gagcgatttc acgaaagcat ctggcgttgc taccgatgat atcgttgctg
     1081 taggtatggt ttaccagttc taatttgatt actaaaagat atgttgcggg aggctttgcc
     1141 tccccaacat ataagtggct ccctcaagcc acttccttta ggagcacaac cttgcttcta
     1201 ac
//
LOCUS       DAZPLI010000362         1187 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZPLI010000362 DAZPLI010000000
VERSION     DAZPLI010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.365"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..755)
                     /locus_tag="ACHUU3_004510"
     CDS             complement(<1..755)
                     /locus_tag="ACHUU3_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFR2084369.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTIGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFSEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINMPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELVEHLKKA"
     gene            1071..>1187
                     /locus_tag="ACHUU3_004511"
     CDS             1071..>1187
                     /locus_tag="ACHUU3_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084370.1"
                     /translation="MYQRCFDNASETLFVVGKTPRLSRFAFSDDPKWESGHHV"
BASE COUNT          290 a          286 c          289 g          322 t
ORIGIN      
        1 gctttcttca gatgttccac cagctcggca atggcctccg gcgaatcgtt atcgagaatg
       61 atgtgcttac gttctgtttg cggcggtacg cgaatgccgg tcagttcagc aagtggcgcg
      121 ctctggctcc agccaatatc acttgcctgc cactgattta ccggtttttt acccgcgccg
      181 agaatggctt tcatcgaagg aatacgtggc atgttaatat cggaggtgac gcagagcacg
      241 gctggaacag agagctcaat aacttcaaca tcatcttcaa gcgtgcgttc aatcaccagt
      301 gtattgccct gacgctgaat agcactcact gcattaatca ccggaagttg cagaatttct
      361 ccgaccagca agccaacctg ctgggcataa aggtcgccgg aaccttcact aaagatcagt
      421 aaatcgaagc cgatcttttc aactgctgcc gccagcgcct ttgcggtatc gagaggcagt
      481 gcatgttcaa gttgcgcatc ctgcaccaaa tacaggctgt gcggcccgcg ggatagcacg
      541 tctttgcgca ctttcgagtt ctgcaacaat gagccaccaa tggtcagcgc ggctatctca
      601 tcgtcatctg ttgcaagctg gcttgcagct tcaatggcat tgagatcgaa ctggctgatt
      661 ttggcgtcgg cattgtcgaa attcagggtg tattctggag tgacaacaat gtcctgttct
      721 tcaggcacca gcttaaagca ggttattatt ttcatgattt ctcctgtgag tcgcatacgg
      781 aaccatctcc gatggaaggt aaaaatggcc tgaaaatagt gtgcgatagc ggtgcggggt
      841 tttcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg cgatcgctaa
      901 aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct tgcattcagt
      961 gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata cacgccgtat
     1021 tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc atgtatcaac
     1081 gctgttttga taacgccagt gaaacgctgt ttgtcgtcgg taaaacgcca cggctttcac
     1141 gttttgcatt tagcgatgat ccaaaatggg agtccggaca tcacgtt
//
LOCUS       DAZPLI010000363         1179 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZPLI010000363 DAZPLI010000000
VERSION     DAZPLI010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.366"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..118
                     /gene="rcbA"
                     /locus_tag="ACHUU3_004512"
     CDS             <1..118
                     /gene="rcbA"
                     /locus_tag="ACHUU3_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="double-strand break reduction protein RcbA"
                     /protein_id="HFR2084371.1"
                     /translation="KLTKSECEKMLSGEKEAGVSREQKVKLINFNCEKLQSS"
     gene            218..433
                     /gene="xisR"
                     /locus_tag="ACHUU3_004513"
     CDS             218..433
                     /gene="xisR"
                     /locus_tag="ACHUU3_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFR2084372.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            435..>1179
                     /locus_tag="ACHUU3_004514"
     CDS             435..>1179
                     /locus_tag="ACHUU3_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFR2084373.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEISSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKG"
BASE COUNT          408 a          213 c          271 g          287 t
ORIGIN      
        1 aaaactaacg aaatcagaat gcgaaaaaat gctctcaggg gaaaaagaag caggcgtttc
       61 cagagagcag aaagtaaaac tgataaattt taattgcgag aaacttcagt cctcgtgaat
      121 tgcattgtat tcaaattaaa acttcatagc tgattattaa taatcaacat cgggcgtcaa
      181 tttcagccta acattggcgc ctgccagagg tgatgcgatg gcacaagtaa tctttaatga
      241 agagtggatg gttgaatacg gcctgatgct tcgcactggt ctgggtgcca gacaaattga
      301 agcataccgc cagaactgtt gggtggaagg cttccacttc aaacgagtat ctcctttagg
      361 gaagccagac agtaagcgag ggattatctg gtacaactat ccgaagataa atcagtttat
      421 caaagactca tgatatgtct aaattaccaa caggtgtcga gattcgaggt aaatacattc
      481 gcatctggtt catgtttcga ggaaaacgat gtcgggaaac attgaaaggc tgggagatta
      541 ctaacagtaa cattaaaaag gccgggaatt taagagcgtt gatagttcat gaaatcagtt
      601 ccggtgaatt tgagtattta agacgttttc cccagtccag cactggggca aaaatggtga
      661 caacgagggt cataaaaaca ttcggggagc tttgtgatat ctggacaaaa attaaagaga
      721 cagagttaac aacaaacaca atgaagaaaa cgaaatcaca attaaaaaca ctcaggataa
      781 taatttgtga gagtaccccg atatcgcata ttcgttatag cgatatctta aactaccgga
      841 atgaactgct gcatggagaa acgctttacc tagataatcc aagatccaac aaaaaaggaa
      901 gaaccgtgcg cacagttgat aactatatcg ccctgctctg ttcgttgtta cgttttgcgt
      961 atcagtcggg atttatatca accaaaccat ttgaaggagt aaaaaaatta cagcgaaaca
     1021 gaataaagcc tgacccgtta tctaaaacag aattcaatgc attaatggaa agtgaaaaag
     1081 gacagagcca aaacttgtgg aaatttgccg tttactccgg gcttcgtcac ggggaactgg
     1141 cagctctggc gtgggaggat gtggatttcg agaagggaa
//
LOCUS       DAZPLI010000364         1177 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZPLI010000364 DAZPLI010000000
VERSION     DAZPLI010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.367"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>1177
                     /gene="rhsB"
                     /locus_tag="ACHUU3_004515"
     CDS             <1..>1177
                     /gene="rhsB"
                     /locus_tag="ACHUU3_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFR2084374.1"
                     /translation="DISHLSEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHET
                     RHAYNAQGLANRCIPDSLSAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRSFG
                     RYELTIAYTPAGQLQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLTG
                     VHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDHHGRL
                     TEKTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVAK
                     RVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVET
                     ATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWL
                     "
BASE COUNT          264 a          374 c          364 g          175 t
ORIGIN      
        1 gacatcagcc atctcagcga agggcaccgg gtgacggtgc attacgggta tgatgagaaa
       61 ggccggctga ccggtgagcg tcagacggtg catcacccgc agacggaagc actgctctgg
      121 cagcatgaga ccagacatgc gtacaacgcg caggggctgg cgaaccgctg tataccggac
      181 agcctgtccg ccgtggaatg gctgacctac ggcagcggct ggctgtcagg catgaaactc
      241 ggcgacacac cgctggtgga gtacacccgc gaccgcctgc accgggaaac gctgcgcagc
      301 ttcggccgtt atgaactcac catcgcttat acccctgccg ggcagttaca gagccagcac
      361 ctgaacagcc tgctgtctga ccgcgattac acctggaacg acaacggcga actcatccgc
      421 atcagcagcc cgcgccagac ccggagttac agctacagca ccaccggcag gctgaccggc
      481 gttcacacca ccgcagcgaa tctggatatc cgcatcccgt atgccacaga cccggcgggg
      541 aaccgcctgc ccgacccgga gctgcacccg gacagcaccc tcagcatgtg gccggataac
      601 cgtatcgccc gtgacgcgca ctatctttac cggtatgacc atcacggcag actgacagag
      661 aaaaccgacc tcatcccgga aggggttatc cgcacggatg atgagcgcac ccaccggtac
      721 cattacgaca gtcagcaccg gctggtgcac tacacgcgga cacaatatgc agagccgctg
      781 gtcgaaagtc gctatcttta cgacccgctg ggccgcaggg tggcaaaacg ggtatggcgg
      841 cgtgaacggg acctgacggg ctggatgtcg ctgtcacgga aaccgcaagt gacctggtac
      901 ggctgggacg gcgaccggct gaccacaata cagaacgaca gaacccgcat ccagacgatt
      961 tatcagccgg ggagcttcac gccactcatc agggtcgaaa ctgccaccgg tgagctggcg
     1021 aaaacgcagc gccgcagcct ggcggatgcc cttcagcagt ccggcggcga agacggtggc
     1081 agtgtggtgt tcccgccggt gctggtgcag atgctcgacc ggctggaaag tgaaatcctg
     1141 gctgaccggg tgagtgagga aagccgccgc tggctgg
//
LOCUS       DAZPLI010000365         1162 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZPLI010000365 DAZPLI010000000
VERSION     DAZPLI010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.368"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            248..493
                     /gene="yahM"
                     /locus_tag="ACHUU3_004516"
     CDS             248..493
                     /gene="yahM"
                     /locus_tag="ACHUU3_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFR2084375.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(510..>1162)
                     /locus_tag="ACHUU3_004517"
     CDS             complement(510..>1162)
                     /locus_tag="ACHUU3_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFR2084376.1"
                     /translation="FMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRR
                     AGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQS
                     TPQMSTLQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWA
                     GIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
BASE COUNT          352 a          277 c          275 g          258 t
ORIGIN      
        1 gtttcgcagc catgcggtgt ataaaaaatg atctcatgca gatgttttgt gaatgtgtgg
       61 gttgacattc atatgaaaaa aatcataatt ccatcatgtt tgtgtggggt cttttctgta
      121 ccttacgcat cgcactcaag cctgacagaa aatatgctgt aaggctcata tcaaaaaccg
      181 ccattagctc atcaggaaga gcagacgaca accaggattg ttgtatggta cggggttcga
      241 ggcctcgatg gcggtccaac ttttcaaaac tcttctgaat cagatcccct tattatcatc
      301 cctgcaaagt ggaacattgc cattgtttgg ctattcagga cgggtcagac caatgaaaaa
      361 agcccacaca ggggagagtg ggctgaaatg ggaagctaaa gactcaagta aacttatcgg
      421 aaataaggac cacgcattac gggggctatc atcgccgata gtcgggattc ggcaaatcag
      481 gttgattacg tagagagtaa attatctgct caccgctgcg tcaccccttc gtaaatcagg
      541 cgtagcgcga atacaccaat aattgcacca ataacccgac tggcaacgcg ttgcatacgc
      601 ccataagcac gacgcacagc gggcaaagaa aacgcctgac taagaaagac tcgccagata
      661 actgatgcga gcacaatccc cgcccaggcc attaaacgtg cccatgttgg tgtttcggca
      721 tttaatgtta ctgagaaaat actgataaaa aataaaacgg tttgcgggtt agagagatcg
      781 gtaattaatc cgcggcgaaa aaagacatac caggaggcgc taatcggttg ttgtagtgtg
      841 ctcatttgcg gtgttgactg gcggcgcatg ctgcaccacg caaaccataa gagataagcg
      901 ccgccgacga ttctgataag cgaaaaaatc tcctcacact gcgttattag cgttgcaaga
      961 ccaaacaaac ccaacccgga ataaaatgca tcgcccagcg ccacgcccag tccggtcagc
     1021 acccctgcgc gtcgaccgga agccaggctg gtttgtacta ccacaaagag attggctccc
     1081 ggattaaaaa aagtaatcac gaacagtcct acggtcaggt aaacggcgtg caaaggatcc
     1141 atagtgattt catccataaa ta
//
LOCUS       DAZPLI010000366         1160 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZPLI010000366 DAZPLI010000000
VERSION     DAZPLI010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.369"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..478)
                     /gene="umuC"
                     /locus_tag="ACHUU3_004518"
     CDS             complement(<1..478)
                     /gene="umuC"
                     /locus_tag="ACHUU3_004518"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR2084377.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQ"
     gene            complement(478..897)
                     /locus_tag="ACHUU3_004519"
     CDS             complement(478..897)
                     /locus_tag="ACHUU3_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR2084378.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT          334 a          294 c          240 g          292 t
ORIGIN      
        1 tctgccgctg ccattttttt gccgcatgat tggcaagctt agccagcgtt ttggtctggg
       61 cgatccccat accaacagta agatgggtac gttgtagcac cgttgcgcga atttctctgc
      121 caaaatcagt cagatcgcga caattacgca cacctgtcag atcgcagaat gcctcatcaa
      181 tactgtaaat ctcgacgcgg ggcgatagtt cttccagcgt cgacatcacc cgattgctca
      241 tgtctgcgta aagctcataa ttgctgctaa agcaaaccac accacagcga cgaaacagat
      301 ctttttgttt gaaccaggga tcgcccattt taacacccag cgcctttgcc tcagcgtttc
      361 gggcgataac gcaaccgtca ttattcgata gcacaaccac cggtttaccc cataaatcag
      421 ggcgaaacac cgtctcacag ctggcataaa acgcgtttac atcacagaga gcaaacatca
      481 gcgcatcgcc ttaacgacgt ggatcaccac accaaagaca tccagcgtat cttcgctact
      541 gatggtaatg ggcgagtacg cactgttcat gggaataagc tgtaccgtcg ggcgtagttg
      601 caattttttc accgtaaact cgccgtcaac agcagcgatg acaatatcac catggctggc
      661 ggtaatagcg ctatcgacaa tcagtaaatc accgtcacta attccaccat caatcataga
      721 atcaccactt gctttgacga agtaagtcgc gctgggatgc tggatcaaca gttgattcag
      781 atcgatgcgc tgttcgacgt aatctgctgc cggtgaagga aagccacact gaacaagatc
      841 gctaaatagc ggaaaagtca caatttcgcg gagatccgca ggcttgataa acaacataat
      901 aatctgcctg aagttatact gtttttatat acagtaatct gttcttgcca gcagatcaat
      961 actgattcag gctatcaata tttgtcgctg catagactgc tgatttttcg ttctcttatc
     1021 ctgtgctcac gtggccttct ggcgacgacg ctcatccagc agaaatgaaa aatatcaccc
     1081 ggcaaaaaaa tagaatagaa gcattcgcca taatgacatt aaacattgtt tgatatttat
     1141 catattaata gaaataaaga
//
LOCUS       DAZPLI010000367         1157 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZPLI010000367 DAZPLI010000000
VERSION     DAZPLI010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.370"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            6..407
                     /locus_tag="ACHUU3_004520"
                     /pseudo
     CDS             6..407
                     /locus_tag="ACHUU3_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..>1157
                     /locus_tag="ACHUU3_004521"
     CDS             572..>1157
                     /locus_tag="ACHUU3_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR2084379.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          322 a          260 c          276 g          299 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgcccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctggc tggcattcca gcaatctgtc tgtgttactc
      721 tcagatatcc caggcgggta ttgtccgttc cgatattgcc tatcagattt atcgtgattt
      781 cgccgaaaac aaagggcttt ttgtacctgg tgccaatgat attccggtat atgataagga
      841 cggaaaactt gtgggaagac tgggtaaagc cccaatggcc gatttcagca gtgtgagctc
      901 aaatggcgtt gctacgcttg tatcgcctca gtatatcgtc agcgtaaagc ataacggagg
      961 atatcggagt gtgagctttg gtaatgggaa aaatacatat tcccttgttg accgtaataa
     1021 ccacccttct attgacttcc atgctccacg tctgaataaa ctggttacag aagttattcc
     1081 ctcagcggta acatcagaag gaaccaaagc caatgcttat aaatacactg aacgttacac
     1141 cgctttttat cgggtgg
//
LOCUS       DAZPLI010000368         1148 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZPLI010000368 DAZPLI010000000
VERSION     DAZPLI010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.371"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHUU3_004522"
     CDS             <1..122
                     /locus_tag="ACHUU3_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084380.1"
                     /translation="HFLPVDQESARVLSTPMKPGNPRRYSNKRTDSSKMERFF"
     gene            119..376
                     /locus_tag="ACHUU3_004523"
     CDS             119..376
                     /locus_tag="ACHUU3_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084381.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            376..750
                     /locus_tag="ACHUU3_004524"
     CDS             376..750
                     /locus_tag="ACHUU3_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR2084382.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            747..>1148
                     /locus_tag="ACHUU3_004525"
     CDS             747..>1148
                     /locus_tag="ACHUU3_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR2084383.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNK"
BASE COUNT          325 a          251 c          317 g          255 t
ORIGIN      
        1 gacattttct gcctgtggac caggaaagtg cacgggtgct ttccactccc atgaagcccg
       61 gcaatccgcg cagatacagt aataaacgaa ctgacagcag taagatggag cgtttctttt
      121 gaacaaccag acaatgactt ttacccctga acaattgcgt aaacacgcgc aggaaatgct
      181 gcgtcatgct gaacaactcg aaaaaacggg cataacaaaa gacgctatcc gtaaagatat
      241 ggtacccgcg cttcgggaac tgatgcaggc gaagcatcgc gcacagaaag cagtagatga
      301 gctggtggac tgtgtggcag agctggaaac cagagtcgga aagtttgaaa aactggtgca
      361 ggaggtgctg cgctgatgcg ccatgagttt attttacctt atccgccgac ggtgaatact
      421 tactggcgac gtcgtgacaa cacatatttt gtatcaaaag ccggtgagcg ttatcgccgg
      481 gatgtggcgc ttattgtccg tcagcagcga ctgaaattaa gcctgtccgg aagactggcg
      541 ataaagatta ttgcagagcc accggataag cgccgtcgtg acctggacaa tatcctgaaa
      601 gcaccactgg atgcactgac acatgcggga gtgttaatgg acgatgagca gtttgatgaa
      661 atcaatatcg ttcgtggtca gccagtatct ggtggacgtc tgggggtgaa gatttacccc
      721 ataatgcatg aagagcaggt caaaaaatga aactggaaga tttaccgaaa tactactccc
      781 caaaatcccc ttgcctgacc gatgcatcgg cctcaacgtc aaaagatgcg ctgagtatca
      841 ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc tgagatgggg ttttctgcgt
      901 tcctggggaa aatgggcatc agtatgaatg acagggcgcg ggcaacagaa ttactggcag
      961 attatgcact cagtcggtgc gatcgtgtgg cggcgttgag aaaacttccg gcagaaataa
     1021 aaccggtagt gatgcgcatt atggcttcgt acgcttttga ggattatgcc cgcagcgcag
     1081 cgagtaaaaa gcagtgccct tgttgctatg gggaaaaatt tattgaaagc gtagttttta
     1141 caaacaag
//
LOCUS       DAZPLI010000369         1145 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZPLI010000369 DAZPLI010000000
VERSION     DAZPLI010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.372"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..187
                     /locus_tag="ACHUU3_004526"
     CDS             <1..187
                     /locus_tag="ACHUU3_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR2084384.1"
                     /translation="REYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNN
                     YRIALRKYQHYQRQTCT"
     gene            320..1120
                     /gene="tcyJ"
                     /locus_tag="ACHUU3_004527"
     CDS             320..1120
                     /gene="tcyJ"
                     /locus_tag="ACHUU3_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR2084385.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT          318 a          248 c          306 g          273 t
ORIGIN      
        1 gcgtgaatat gtcgttcgtc tgcgccgttt gggtaatcat ctacacgagc aaaatatttc
       61 cctcgatcag ctgcaggacg gtttccttga tgaaatcctc gccccgtggc tgcccaccac
      121 cagcaccaac aattaccgca ttgcgttacg gaagtatcaa cactatcagc gtcaaacgtg
      181 tacctgactt gtgcagaaat cctccagtct gcctgcttct gatatatatt aaataagaat
      241 aagatgtagc ggagttgttt ttgtgtttac aaacaatggc tctacactgc aaacagacat
      301 aacaacattc ggggtgaata tgaaattagc acatctggga cgtcaggcat tgatgggtgt
      361 gatggccgtg gcgctggttg cgggtatgag cgttaaaagt tttgcagatg aaggtctgct
      421 taataaagtc aaagagcgcg gcacgctgct ggtagggctg gaaggaactt atccgccgtt
      481 cagttttcag ggggatgacg gcaaattaac cggttttgaa gtggaatttg cccaacagct
      541 ggcaaaacat cttggcgttg aggcgtcact aaaaccgacc aaatgggacg gtatgctggc
      601 gtcgctggac tctaaacgca ttgatgtggt gattaaccag gtcaccattt ctgacgagcg
      661 caagaaaaaa tacgattttt ctacccctta caccatttcc ggtattcagg cgctggtgaa
      721 aaaaggtaac gaaggcacca ttaaaacagc cgatgatctg aaaggcaaaa aagtgggtgt
      781 cggtctgggc accaactatg aagagtggct gcggcagaat gttcagggtg tcgatgtgcg
      841 tacctatgat gatgacccga ccaaatatca ggatctgcgc gtaggccgta tcgatgcgat
      901 cctcgttgac cgtctggcgg cgctggatct ggtgaagaaa accaacgata cgctggcagt
      961 aaccggtgaa gcattttccc gtcaggagtc tggcgtggcg ctgcgtaaag gaaatgaaga
     1021 tctgctgaaa gcagtgaatg atgcaattgc ggaaatgcaa aaagatggca ctctgcaagc
     1081 cctttccgaa aaatggtttg gtgctgatgt gaccaaataa tcagcataat gacaaaaaag
     1141 ggcgc
//
LOCUS       DAZPLI010000370         1140 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZPLI010000370 DAZPLI010000000
VERSION     DAZPLI010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.373"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>1140
                     /locus_tag="ACHUU3_004528"
     CDS             <1..>1140
                     /locus_tag="ACHUU3_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR2084386.1"
                     /translation="EREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEADLNRLQKES
                     DTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTA
                     EQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLTSKYAE
                     HTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPMEEALHGYVLTDGKLHVDDTPV
                     PVLLPGNKKTKTGRLWAYVRDDRNAGSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQ
                     ADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALTEEALKRIGELYAIEAEI
                     RGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELAKAFA"
BASE COUNT          250 a          323 c          361 g          206 t
ORIGIN      
        1 ggagcgcgaa atagaccatc tgcaggcgca gctggataaa ctccgccgga tgaacttcgg
       61 cagtcgttcc gaaaaagtct cccgccgtat cgcacaaatg gaagccgatc tgaaccggct
      121 tcagaaagag agcgatacgc tgactggtag ggtgtatgac ccggcagtac agcgtccgtt
      181 gcgtcagacc cgcacccgta agccgttccc tgaatcacta ccccgtgacg aaaagcgact
      241 gttgcctgcg gcgccgtgct gcccgaactg cggcggttca ctgagctatc tgggcgagga
      301 taccgccgaa cagctggagt tgatgcgtag cgccttccgg gttatccgga cggtacggga
      361 aaaacatgcc tgtactcagt gcgatgccat cgtgcaggca cctgcacctt cgcggcccat
      421 cgagcggggt atcgccggac cggggctgct ggcccgcgtg ctgacctcga agtatgcaga
      481 gcacaccccg ctgtatcgcc agtcagaaat atacggccgg caaggtgtgg agctgagccg
      541 ttcactgctg tcgggctggg tggatgcatg ctgccggctg ctgtctccga tggaagaggc
      601 gcttcatggc tatgtgctga ctgacggtaa gctccatgtt gatgacacgc ctgtcccggt
      661 gctgttgcca ggcaataaga aaacgaagac cgggcggttg tgggcgtatg ttcgtgacga
      721 ccgtaacgcc gggtcaacgc tggcgccggc ggtgtggttc gcttacagcc cggacagaaa
      781 aggcatccat ccgcagaccc atcttgcggg gttcagtggt gtactgcagg cggatgcata
      841 cgccgggttc aacgagctgt accgggatgg ccggataacg gaagccgcct gttgggctca
      901 cgcccgccgt aaaatccacg atgtgcacgt tcgcaccccg tcagccctga cggaggaagc
      961 gctgaaacgg atcggcgaac tgtacgccat cgaggcagag ataaggggaa tgacggcgga
     1021 acagcgcctt gccgaacgtc agttgaaaac gaaaccgctg ctgaaatccc tggaaagctg
     1081 gctgcgtgaa aagatgaaaa ccctgtcgcg acactcagaa ctggcgaaag cgttcgcata
//
LOCUS       DAZPLI010000371         1134 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZPLI010000371 DAZPLI010000000
VERSION     DAZPLI010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.374"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..94)
                     /gene="yqcG"
                     /locus_tag="ACHUU3_004529"
     CDS             complement(<1..94)
                     /gene="yqcG"
                     /locus_tag="ACHUU3_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HFR2084387.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDN"
     gene            233..904
                     /gene="queE"
                     /locus_tag="ACHUU3_004530"
     CDS             233..904
                     /gene="queE"
                     /locus_tag="ACHUU3_004530"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR2084388.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          316 a          230 c          252 g          336 t
ORIGIN      
        1 cgttatcatt aaaaactaat acagaaaaaa taaatataat tttggtgaat gttttgacat
       61 gattaaatcc attttcttta ttttcttctg acataatacg ttactccctt taattattaa
      121 cgatatgttt cgcatttttc tttaaatctg cctgtaacag aatgttaata agacatgatt
      181 gttgtggcag gatctgcaat aattaacaat tatccacttc acagagaatg ctatgcagta
      241 cccgattaac gagatgttcc agaccctgca aggtgagggt tactttaccg gcgtccccgc
      301 catttttatt cgtttacagg gatgcccggt tggctgtgcc tggtgcgaca ccaaacacac
      361 ctgggaaaag cttgaggatc gggaagtctc ccttttcagc attctggcga agaccaaaga
      421 gagtgataag tggggggcgg cgagcagtga agacttgttg gctgtcatta gtcgccaggg
      481 atacaccgcg cggcatgtgg tgattacggg tggtgagcct tgcattcatg atttgctacc
      541 actgactgat ctgctcgaaa agaacggttt tagctgccag atcgaaacca gtggtactca
      601 tgaggtacgc tgcacaccga atacctgggt taccgtatcg ccaaagctga acatgcgcgg
      661 cggctatgac gtgttgtcac aggcactgga gcgagccaac gaaatcaagc atccagtggg
      721 gcgcgtacgc gatattgaag cactggatga actattggca acgctgaccg atgataaacc
      781 gcgagtcatt gcactgcagc cgattagcca aaaggatgat gccacacgtt tgtgcattga
      841 aacctgcatt gcgcgtaatt ggcgtttgtc gatgcaaaca cataaatatc taaatattgc
      901 ctgattaaac atttataagc gttataaatg ggtggaacct gtaaagactt ctactcattt
      961 atattgtttg tcgcctctga aaactcctcc attttaccca tccagggcta atcattagca
     1021 ttctctacaa attctgtggc attaattttt cgctggagtg aaaattattg cggtaaagtt
     1081 tggtagattt taatttgtat agagttattt taaatattta cctttttaat caat
//
LOCUS       DAZPLI010000372         1117 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZPLI010000372 DAZPLI010000000
VERSION     DAZPLI010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.375"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            84..1115
                     /locus_tag="ACHUU3_004531"
     CDS             84..1115
                     /locus_tag="ACHUU3_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR2084389.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          301 g          228 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttatca gccggtttac tcgccatggg
      961 tgaatcatgt tgaacggcta tggcaggcac ttcacgacac aataacgcgt aatcatcagt
     1021 gcagctcaat gtggcaactg ttgaaaaaag ttcgccattt tatggaaacc gtcagcccat
     1081 tccccggagg caaacatggg ctggcaaaag tgtagcg
//
LOCUS       DAZPLI010000373         1091 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZPLI010000373 DAZPLI010000000
VERSION     DAZPLI010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.376"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..594)
                     /locus_tag="ACHUU3_004532"
     CDS             complement(<1..594)
                     /locus_tag="ACHUU3_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084390.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYACFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
BASE COUNT          335 a          237 c          179 g          340 t
ORIGIN      
        1 cagcttagcc agtcttgatt tcagcaaatt aatctcattt tctcttgctt tcatcagttt
       61 ctctttggca atttcttcaa gctcagcttg tgatttatca gcaacaatta gagacagtcg
      121 atgtggtgct ctgacagctc tttctgtacg aacaactctt tgaccttctg gtaaatctgc
      181 aatcacttta ccaatctcta cagggtcata cccccaaccg tcacggtcta gtcgagagaa
      241 acaagcataa gaattggttt gaagctccat cgaaataacc atttcgtcaa tttcgtcttc
      301 aagcttggac tgaattttct cgtaaacact tctcattgct tcttgagagt ctttaatagc
      361 ttggaaatat cggcgttgta gcttcaactt caaatcaagt tcacaccgtt cgttcataga
      421 aaaatcaatt tcaactcttg gaatctcgag tggttctggt ggaatatatg gacctttatt
      481 tacttcgtgt gattcccgcc aagctctatc aaaagattca cgcaaagtta attcgtgaat
      541 atcaagtgca cttggtctgt gttcgataag catttcatgt tcatttctaa tcattcgttc
      601 accccttagt ttatttgtta tttcaattta tagaagcaca ctcagcaatc agaggtgaag
      661 agtctgactg aacaagaacc tgccaagtgt gcttttaaaa actgaaaaag ccctaaacat
      721 ttctgtgaag ggcaaaggat tcaataatga atgaacaaga gtactttttg agatagcctc
      781 tcagactaac ctgaaaggcc atgacgaaaa gcattgcacc tttccaactc agaatatcca
      841 tccatcggat tattgaaaga aaaataagag agaattgctc tcccataatt aaaattatgc
      901 catgcttaag gttatttgtc aacagctttt taacaggttg ataacatcaa ttcttattta
      961 ccgcattgtt aataatgcaa ccaacaaata agccaagcaa cgtccaagca attattaaac
     1021 ccattagtga accctcacag aatccgtcac cagaatatta tcactgtaca ggattgtcac
     1081 agcacctttc a
//
LOCUS       DAZPLI010000374         1090 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZPLI010000374 DAZPLI010000000
VERSION     DAZPLI010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.377"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            214..>1090
                     /gene="nadA"
                     /locus_tag="ACHUU3_004533"
     CDS             214..>1090
                     /gene="nadA"
                     /locus_tag="ACHUU3_004533"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR2084391.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT          294 a          259 c          265 g          272 t
ORIGIN      
        1 ttcaggcatc ctcaatttca ctttgtaaac ctgatgacat cgtcagagct tactgtgcaa
       61 gcaactctac gtcggtggaa ttaggcgtaa ttcgagtgac ttttccccac cattcgacta
      121 tcttgtttag catataaaac aaattacgcc gatagtagcg aatattacgc taatgtcggt
      181 tttaacgtta agcctgtaaa acgagatggt aagatgagcg tgatgtttga tccagacacg
      241 gcgatttatc ctttcccccc gaagccgacg ccgttaagca ttgatgaaaa agcgtattac
      301 cgcgagaaga taaaacgtct gctaaaagaa cgtaatgcgg tgatggttgc ccactactat
      361 accgaccccg aaattcaaca actggcagaa gaaaccggtg gctgtatttc tgattctctg
      421 gaaatggcgc gcttcggtgc aaagcatccc gcttctacgt tgttagtcgc tggggtgaga
      481 tttatgggag aaaccgccaa aattctcagt ccggaaaaaa caattctgat gctgacactt
      541 caggctgaat gttcactgga tctcggctgc cctgttgaag aatttaacgc attttgcgat
      601 gcccatcccg atcatactgt cgtcgtctac gccaacactt ctgctgcggt aaaagcgcgc
      661 gcagattggg tggtaacttc aagcattgcc gtcgaactta ttgatcatct ggatagtttt
      721 ggtgaaaaaa tcatctgggc acccgacaaa catctggggc gttacgtgca aaaacagacg
      781 ggcgcagaca ttttatgctg gcagggtgcc tgtattgtgc atgatgaatt taagactcag
      841 gcgttaaccc gcttgcaaga agaatacccg gatgctgcca tactggtgca tccagaatca
      901 ccacaagcta ttgtcgatat ggcggatgcg gtcggttcca ccagtcaact gatcgctgct
      961 gcgaaaacat tgccacatca gcggcttatc gtggcaaccg atcggggcat tttctacaaa
     1021 atgcagcagg cggtgccaga taaagagtta ctggaagcac caaccgcagg tgagggtgca
     1081 acctgccgca
//
LOCUS       DAZPLI010000375         1087 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZPLI010000375 DAZPLI010000000
VERSION     DAZPLI010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.378"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            35..190
                     /locus_tag="ACHUU3_004534"
                     /pseudo
     CDS             35..190
                     /locus_tag="ACHUU3_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            294..>1087
                     /locus_tag="ACHUU3_004535"
     CDS             294..>1087
                     /locus_tag="ACHUU3_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR2084392.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSL"
BASE COUNT          315 a          223 c          254 g          295 t
ORIGIN      
        1 ataacagatg tctggaaata taggggcaaa tccaggtact gccgaaaata ttcagataga
       61 gctgagggat gaccaggatg ctgcgttaaa aaatggcgat agcaaaacgg ttattgttga
      121 tgagatcact cgtaatgcac agtttccact taaggcaaga gctatcacgg tgaatggaaa
      181 cgcaagctag ggaacgatcg aggcgctaat caatgtgatc tacacctggc aataacgtcg
      241 attcgatttt cttagccttc tttttaaatc aatttttaac cttaggatat atcatgggta
      301 aaacaatcag tataaaggtc ctgttcggta tttatcttct attaatggca ggcaaagttt
      361 ttgccttcag ttgtaatgtc gatggcgggt caagtattgg agcaggtaca acctctgttt
      421 atgttaatct cgaccctgta atacagccgg gccagaatct ggttgtagac ttgtctcagc
      481 atataagttg ctggaatgat tacggcggct ggtacgacac tgatcatata aacctggtac
      541 aaggctcagc tttcgcaggc tccctacagt catataaagg atcgctgtac tggaataatg
      601 tgacctaccc cttcccgttg acgacaaata ccaacgtatt ggatattggt gataagacac
      661 cgatgccatt accgttaaaa ctctatatta ccccagttgg ggcggccggt ggagttgtca
      721 taaaagcggg ggaagtcatt gcccgtattc atatgtataa aattgcaaca ttaggaagcg
      781 gaaatccgcg taattttacg tggaatatta tttcaaataa cagtgttgtt atgccaacag
      841 gcggctgcac cgttgatagt cgtaatgtca ctgtggatct cccggacttt cctggaagcg
      901 cagaaatacc ccttggcgta tattgctcaa gcgaacaaaa gctctcattt tatctttcgg
      961 gtgccaccac tgacagtagc cgacaagtgt ttgctaacac agcacctgat gcaacaaaag
     1021 catcgggagt gggtgtcact ctaatgcgta atggaaaaat actggctacg ggagaaaatg
     1081 tctccct
//
LOCUS       DAZPLI010000376         1085 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZPLI010000376 DAZPLI010000000
VERSION     DAZPLI010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.379"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..643)
                     /locus_tag="ACHUU3_004536"
     CDS             complement(<1..643)
                     /locus_tag="ACHUU3_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR2084393.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIT"
     gene            complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="ACHUU3_004537"
     CDS             complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="ACHUU3_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR2084394.1"
                     /translation="YLISEDMTEPHEARRTEYHARYLDIQIVLKGQEGMTFSTQPAGT
                     PDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKM
                     LMA"
BASE COUNT          312 a          247 c          191 g          335 t
ORIGIN      
        1 ccgtaatcgt agaatcaact gaatctttac aactcacctg cgagttattc aagtcctgag
       61 aattatttaa ctcctgcgga atatttaata gacgagctat ttccttgtta gcttcgcgtt
      121 ggtaacatgc cagaatcttg ttagtaatca ggtgtaatga actctcatct tttctttcaa
      181 aggactttcc ttcataacga ttagtcgaac catcaggttg ttgaaatata atagccgtat
      241 gtccttgacg agtccccata gtagtgaaag cggaaagtgt gatttctatt tccaccttag
      301 ataaatccgc tcgttcccct tcaggattat tcataacaaa cttcattatc tcactgccat
      361 cattattttt gatataagat gaggactcaa tatgagccat catatttctt cccgcttcgt
      421 tggagcgttt gctaaaatcc acataaccta gagtaaatat ttttaccaaa aattccaaaa
      481 atgtcttagg atcaaggata tttcttgtgt tatcctgccc ctcggcatta cgggcaaaat
      541 tattcattcc aacacgccag gtatttaaaa cttcactttt ttgtttaaaa ttgtcggcta
      601 atgtaatgtg ttgagaataa ttcaaatctg atattttgct catgacgaac tccttgagtt
      661 aataagaacg tctaagcatt acaaatttta ctcacaaaaa aatatcaaaa aacgctcttt
      721 tcacaaactc gccgtaaacc tggagttatt tacggcgaaa agtcatcacg ccatcagcat
      781 cttcactact gctttgcgaa cctgggctgg tgcacccact gcgcacagcg gtttatgcac
      841 ttcccccgga taaaacacaa caaaatcacc ttcattaagg ataactgttt tctcatcaac
      901 gccttccggc aaaaatgcga tgtctttatc agctaaccag tcggtatccg gcgtgcctgc
      961 aggttgcgtg ctgaaggtca tgccttcctg accttttaac acaatctgaa tgtcgagata
     1021 gcgggcatgg tactccgtac ggcgagcttc gtgcggctcg gtcatatctt ccgagataag
     1081 ataaa
//
LOCUS       DAZPLI010000377         1068 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZPLI010000377 DAZPLI010000000
VERSION     DAZPLI010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.380"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..581)
                     /locus_tag="ACHUU3_004538"
     CDS             complement(<1..581)
                     /locus_tag="ACHUU3_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014606218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR2084395.1"
                     /translation="MARISKKESANHNKVMELIHSDKLLTQEDREFIFTNYRGDGIGA
                     TGAFFTPEILAWDFVLDADCTGDCIELCAGIGRLSYYQYLRNKPTHITCVELNPEYVM
                     IGKRVLPEAEWITGNALQYSPDRFYDVAYGNPPFGKISTSKAYTGRYKGSEFEYKVIE
                     HASTFASYGAWIVPQGSAGFVYSGVHCYERRES"
     gene            616..>1068
                     /locus_tag="ACHUU3_004539"
     CDS             616..>1068
                     /locus_tag="ACHUU3_004539"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084396.1"
                     /translation="MDKKGIFLIIALLIFILLMLLLFAKLNFNVNTALAILSLILTVL
                     SGTDLVKKYTTVKSTNKSPIDMWMVFGIAISAVSMYGLYIDPQLSPLEGLTICIVSLI
                     LFVSVLVSALLNYKKIKKPDIREIIKQSHSVFRENEETITITTTIKKAP"
BASE COUNT          326 a          220 c          178 g          344 t
ORIGIN      
        1 catgattctc tacgctcata acaatgtacc ccactgtaga caaagcctgc ggaaccttgc
       61 ggtactatcc atgcaccata ggacgcaaag gtacttgcat gttcaatcac cttgtattca
      121 aattcactac ccttataacg gcctgtatac gcttttgagg tactgatctt cccaaagggt
      181 ggattaccat aggcaacatc atagaaacgg tctggtgagt actgtagtgc gttaccagtg
      241 atccattctg cctctggtag taccctcttc ccgatcatta cgtactcagg attgagttcc
      301 acgcatgtaa tatgcgttgg tttattcctt aagtactggt agtagctgag gcgaccgata
      361 ccagcacata gttcaataca gtcaccagta cagtcagcat caagtacaaa atcccatgct
      421 aaaatttcag gtgtgaagaa ggcacctgta gcccctatcc catcccccct ataattggtg
      481 aatatgaatt ccctatcttc ttgtgttagg agcttatctg aatgtattaa ttccatcact
      541 ttattgtgat tagcagattc ctttttactt atccttgcca taagatacct tttttaaaaa
      601 tacgacgggg tatttatgga caagaaagga atatttttaa tcatagcgtt acttattttt
      661 attttactaa tgttgctttt attcgctaag ctaaatttta atgtaaatac tgccctcgct
      721 atattatcat taattttaac agtgctatca ggtacggact tagtcaaaaa gtacactacg
      781 gtcaaatcga caaataaaag ccctatagat atgtggatgg ttttcggtat tgctatctcc
      841 gctgtttcta tgtatggatt atatatagac ccccaactat cacctttaga aggtttaact
      901 atttgtatag tatcacttat actttttgtt tctgttttag tttcggcttt attaaattac
      961 aaaaaaataa agaaaccaga tattagggaa ataataaaac aaagccattc ggtttttaga
     1021 gaaaacgaag aaaccattac tattacaaca accataaaaa aagcccca
//
LOCUS       DAZPLI010000378         1062 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZPLI010000378 DAZPLI010000000
VERSION     DAZPLI010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.381"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..906)
                     /locus_tag="ACHUU3_004540"
     CDS             complement(<1..906)
                     /locus_tag="ACHUU3_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HFR2084397.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
BASE COUNT          302 a          241 c          210 g          309 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt
     1021 tgatgaataa tatcagtgcg tcataattca agttaataac ct
//
LOCUS       DAZPLI010000379         1049 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZPLI010000379 DAZPLI010000000
VERSION     DAZPLI010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.382"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..120
                     /locus_tag="ACHUU3_004541"
     CDS             <1..120
                     /locus_tag="ACHUU3_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR2084398.1"
                     /translation="EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            120..410
                     /locus_tag="ACHUU3_004542"
     CDS             120..410
                     /locus_tag="ACHUU3_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR2084399.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            407..961
                     /locus_tag="ACHUU3_004543"
     CDS             407..961
                     /locus_tag="ACHUU3_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR2084400.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          299 a          201 c          298 g          251 t
ORIGIN      
        1 gagtttcagc aggtttataa atctgttctg aatgtgctgt ggaactggat tctgttccgt
       61 aaattttcct ctccggaaca agtcgaaaat gtggccgcgc agctgctgga gtttgcgtaa
      121 tggtggattt acgtaaagcg gcgcgggggc agatgtgcac cgtcagaatt cctggctact
      181 gcaatcacga tccggaaacg tctgtgctgg cgcattaccg actggcggga acgtgcggaa
      241 cagcgataaa gccacacgat atgcaggcag cgattgcctg tagctcgtgc cacgatttaa
      301 tcgacgggcg ggtaaaaacc agcgattaca ccaaagaaga attacgcctg atgcatgcag
      361 aaggtgtttt tcgcacacaa gaaatctgga gaaaggaggg atatttatga tttacccaac
      421 gaatacagga aaaagcggag aacaccttcg tctcaccacg ctggaaagtg tctggattca
      481 gggaaaacta cgtatgtggg ggcgctggtc gtatattggt ggcggtaaga cggggaatat
      541 gtttaacctg atgttgacct ctaaaaagct gacaaaaacg gcaattaacg aggcgctccg
      601 gaggatgaaa aaagcaggtc tgaacaagtc tgaacttgag gcttttttgc gggatatgat
      661 taacggtaag caaaagagct ggctggcgca ttgtactgat gcagaggcgt tatgtattga
      721 tcgggtcata agtgaggtgc tggcagagca tccaggattg attagcgtcc ttcggcaacg
      781 gtatgagggg cgggggatga ccaaacgcaa aatggctgaa ctgctgaatg atgcacaccc
      841 gaaatggagt ttaagaacct gtgaaagacg cattgagcat tggctaaagg tggcagaatt
      901 tattttgtac aaaccaatgg ttatggcttt tggtatagag aaaaaagtta ttgctttttg
      961 acgtaaaaac tgcttcaatt cttgtacgct tcgcaaagct gtaccgcgag gcgaatagca
     1021 gacatggaca tttgaaagag cccgcttta
//
LOCUS       DAZPLI010000380         1046 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZPLI010000380 DAZPLI010000000
VERSION     DAZPLI010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.383"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            108..257
                     /locus_tag="ACHUU3_004544"
                     /pseudo
     CDS             108..257
                     /locus_tag="ACHUU3_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            284..388
                     /locus_tag="ACHUU3_004545"
                     /pseudo
     CDS             284..388
                     /locus_tag="ACHUU3_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            728..985
                     /locus_tag="ACHUU3_004546"
     CDS             728..985
                     /locus_tag="ACHUU3_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFR2084401.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          357 a          180 c          198 g          311 t
ORIGIN      
        1 atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac
       61 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
      121 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
      181 atctgaagct tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg
      241 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
      301 tatcgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
      361 acaatttgaa aatcagaatc tcacttaaag cggcatccat tttacaaaag atggccgtct
      421 gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata
      481 tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga
      541 gttgactgaa tataatacag cctcttccta cataagaagg aatgtgctca caaaaaaaaa
      601 ccattagtct gcttcctgtt cttacaataa tcagtattat gtattttatt tatatagcaa
      661 aatatgcttg ttacacctgt cataattagg catggtaaaa ttattgactg aaccctgggg
      721 gaggaagatg cacagtgatt attataatat ggtttttggt gaaaaattgg ccaatatttt
      781 atatgaggct aactctcaat ttttttacga gagaaatgtc atagaagagg cagtcaacgc
      841 tttattctgt gagcgtgaaa taataaacaa caagaacata ataaagaagc tgatgttctt
      901 tttgtcagat gttaatcaca ccaagaaaga tgttgttcag agcgcactta atattatcat
      961 tgacattaca tcaggtgata tttgagattt tacataaaag ataatctctc aaggagtctt
     1021 atctgaaaac gaaatatata gtccct
//
LOCUS       DAZPLI010000381         1038 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZPLI010000381 DAZPLI010000000
VERSION     DAZPLI010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.384"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            9..944
                     /locus_tag="ACHUU3_004547"
                     /pseudo
     CDS             9..944
                     /locus_tag="ACHUU3_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238933.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            946..>1038
                     /locus_tag="ACHUU3_004548"
     CDS             946..>1038
                     /locus_tag="ACHUU3_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFR2084402.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSN"
BASE COUNT          337 a          188 c          266 g          247 t
ORIGIN      
        1 acacgtaggg tgaagccggg ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg
       61 tcacgcagag tcagtttgac gccgtgggca ggctcaggac acagacggat gccgcaggcc
      121 ggacaacaga atacagcccg gatgtggtga cgggtctcat cacgcgcatc accacgccgg
      181 atggcagggc atcggcgttt tactataacc accacagcca gttaacgtca gccaccgggc
      241 ctgacgggct ggaaatacgc cgggaatatg atgaatgggg caacctgctg aatgaagaga
      301 acccgcatca gctgcagcag cttattcgtt tgccggggca gcagtatgat gaggagtccg
      361 gcctgtatta taaccgccac cgctattatg acccgctgca ggggcggtat atcactcagg
      421 atccgattgg gctgaagggg ggatggaacc tgtatacata tccgctgagc ccggtgaata
      481 gcatggatcc attaggatta tatgaattta aatcaaaaaa tattgatgat attggaatat
      541 ttgcattggc aatgtgtaat ggagaatcaa ttaacgagaa taaagaatat ggtggactaa
      601 tatgtaagaa gcaaggtgaa tatttcccca tgaatccgat aagttcaaat gataatgata
      661 gtgtagactt gcgaaatata aaatgccctg aaggttcaga gagagtaggc gattatcaca
      721 ctcacggttt ttactctgac gataaaggaa ataaagtaac aaaagaaaat gatgtttatg
      781 atagtctaaa tttttcaagc aaagatttaa cgaattctta tatgaatgga atgggaaaaa
      841 aagaatacag tagttacttg ggaacaccaa ataacaccta tctaaaatat aatcccaaag
      901 ctaaagggaa tggagttaca attatcaggc aagggagtaa ttaaaatgaa ggcaatacaa
      961 tgttgttatt tccttgcttt agtctccagt catttaatgg ctggagttat gggtgatgat
     1021 aatatatttt taagtaat
//
LOCUS       DAZPLI010000382         1028 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZPLI010000382 DAZPLI010000000
VERSION     DAZPLI010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.385"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            228..>1028
                     /locus_tag="ACHUU3_004549"
     CDS             228..>1028
                     /locus_tag="ACHUU3_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR2084403.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPVFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAKNAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLE"
BASE COUNT          353 a          211 c          177 g          287 t
ORIGIN      
        1 attatgggaa ataaataaaa ggtaattcat ggatggttga actatatccc tgcttttcaa
       61 ccatcctaaa aaagcatgaa ttgctcgctc taccctaagt ggctgtgcta ttttagaaaa
      121 acttaatcct gagttaagca aaaaaacata atcaataaaa atacatacgc aaaaaacatt
      181 gattaagtga atataccatg gaagaaaaat ataaaccgga gtagtgtatg gccaatctct
      241 acgacttgaa aaaattcgac ttaaatcttc ttgtcatatt tgagtgtatt taccagcatt
      301 taagcattag caaagctgct gaatcactat atatcacccc atcagccgtg agccagtcgt
      361 tacaacgctt acgtgcgcaa tttaatgacc ctgtattcat tcgctcagga aaaggtatcg
      421 cgccgacgac aacagggttg aatctgcatc accatcttga gaaaaacctc aaaggccttg
      481 aacaaacaat taatatagtc aataagtccg aactaaagaa aaactttatt atctacggcc
      541 ctcagctcat ttcttgctct aataatagta tgctgatccg ctgcctacgg caagatgcct
      601 ctgtcgagat tgagtgccat gatatcctca tgtcagcaaa gaatgctgaa gaactgttgg
      661 ttcaccgcaa agccgatctg gtgatcaccc aaatgccagt gatcagccgt tccgtcattt
      721 gcatgccatt gcatacaatt cgcaatactc taatttgtag taataagcat ccgcgaatta
      781 ctgacaatag cacttatgaa caaattatgg cagaagagtt cacccaatta atatccaaat
      841 cagcaggagt tgatgatatt caaatggaaa tagacgagaa gtttatgaat cgcaaaattt
      901 catttcgtgg ttcctctttg ctgacaataa ttaatagcat tgcagttaca gatttactgg
      961 gaatagtccc ctatgaactt tataactccc accgtgactt tcttaattta aaagagataa
     1021 aactagaa
//
LOCUS       DAZPLI010000383         1023 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZPLI010000383 DAZPLI010000000
VERSION     DAZPLI010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.386"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(77..865)
                     /locus_tag="ACHUU3_004550"
     CDS             complement(77..865)
                     /locus_tag="ACHUU3_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR2084404.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="ACHUU3_004551"
     CDS             complement(905..>1023)
                     /locus_tag="ACHUU3_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR2084405.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAZPLI010000384         1009 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZPLI010000384 DAZPLI010000000
VERSION     DAZPLI010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.387"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..164
                     /locus_tag="ACHUU3_004552"
     CDS             <1..164
                     /locus_tag="ACHUU3_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR2084406.1"
                     /translation="SLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAH
                     HQVDIDLED"
     gene            170..>1009
                     /locus_tag="ACHUU3_004553"
     CDS             170..>1009
                     /locus_tag="ACHUU3_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR2084407.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSILIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIEFLNLGGEHLGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIA"
BASE COUNT          306 a          229 c          246 g          228 t
ORIGIN      
        1 aatcgctctg catcgaccat ttgcaacgcc acggtgccaa taaacaggca atcagccacg
       61 catttgatga tgatgttgag tttcaggagc gcatggcaga acacatccgg tacatggctg
      121 aaaccattgc ccaccaccag gttgatattg atttggagga ctaaaatcta tggcgctttt
      181 tcaacgagca ataaacacac aggcctatct aaaagcagga attatggggt ttgctggcga
      241 cggcaaaacc tacacagcaa gcgaactggc tattggtctg gtaatgctta tgcggcaacg
      301 gggaattgaa gctggtaacc gcccggtaat gtttctggac acagaaacag gctctgactg
      361 ggttaaaccc cgctttgatg cggaaaatat cgaattattt acggcaaaaa cccgctcgtt
      421 tgttgatctg cttgaagcca ttaatgaagc ggaaagcagc ggttccgtaa tgatcatcga
      481 ctccatcagt cacttctgga ccggattatg cgacgagtat gccagacgcc gaaaccgtaa
      541 acgaggactg gaattttcag actgggcgtg gctcaaacag gagtggcgac gctttaccga
      601 tcgatttgtt aacagtcagg cgcacattat tatgtgcggg cgtgcaggat atgagtatga
      661 ctttttcgaa ggtgatgatg gcaaacgcca gttggcaaaa accggtataa aaatgaaagc
      721 agaaacagaa accggttatg aaccctccat cctgatccag atggagaagc aaatgaacat
      781 agaaaccggt caggtgtggc gcaccgccag aatactaaaa gatcgctcca cccgtattga
      841 tggacaggta ttttcaaacc caacatttaa aaactttctt cctcatatcg agtttcttaa
      901 cctgggaggt gaacacctcg gcatcgatac ctcacgggat aacagtgaat tatttgccaa
      961 cgacggtaca ccgacatggc taaaagaaaa gcgtgcaaaa gaaatcgca
//
LOCUS       DAZPLI010000385         1009 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZPLI010000385 DAZPLI010000000
VERSION     DAZPLI010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.388"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..126
                     /gene="rcbA"
                     /locus_tag="ACHUU3_004554"
     CDS             <1..126
                     /gene="rcbA"
                     /locus_tag="ACHUU3_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break reduction protein RcbA"
                     /protein_id="HFR2084408.1"
                     /translation="SKSKLTKSECEKMLSGKKEAGVSREQKVKLINFNCEKLLSS"
     gene            231..455
                     /locus_tag="ACHUU3_004555"
     CDS             231..455
                     /locus_tag="ACHUU3_004555"
                     /inference="COORDINATES: protein motif:HMM:NF019442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR2084409.1"
                     /translation="MYLTLKEWNARQLRPRSPETVRRWVRECKIFPPPIKDGREYLFH
                     ESAKKITPQTTGGLLQRIRNGRTKKKLKHT"
     gene            346..>1009
                     /locus_tag="ACHUU3_004556"
     CDS             346..>1009
                     /locus_tag="ACHUU3_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFR2084410.1"
                     /translation="MNIYFMSQQKKLHLKRLAGFCRESEMAGRRRNSNTRDLPPNLYN
                     RGGYYSYRDPRSGREFGIGRDKRIAVNEAIAANMELLSTQHISLLDRIKGNSVTLFCE
                     LILNFRNEIERRQLSTNTMKRHNQRLKIISEYFGGVPVKNIGIREVYSFLEIRAAGSK
                     FAIANQYRALLSDIFKTAIASGLAEEDPASATRPFRTEVKRSRLLIDEYLLIRKIADV
                     QNE"
BASE COUNT          357 a          184 c          219 g          249 t
ORIGIN      
        1 tcaaaatcta aactaacgaa atcagaatgc gaaaaaatgc tctcagggaa aaaagaagca
       61 ggcgtttcca gagagcagaa agtaaaactg ataaatttta attgcgagaa acttctgtcc
      121 tcgtgagttg cattatatca aaattagaac ttcatagctg attattaaaa atcaaccacg
      181 cccgccagta ttttgtatat ttactggcgg tcatattaca ggaatatgac atgtatttga
      241 cactcaaaga atggaacgcc cgtcagttac gtccacgaag cccagaaacg gttcgccgct
      301 gggtgcgaga atgtaagatt tttccaccac cgataaagga tgggcgtgaa tatttatttc
      361 atgagtcagc aaaaaaaatt acacctcaaa cgactggcgg gcttttgcag agaatcagaa
      421 atggccggac gaagaagaaa ctcaaacaca cgtgatctac cacccaatct ttacaatcga
      481 ggtggatatt atagttatcg tgacccacgt tcaggaaggg agtttggaat cggaagagac
      541 aaaagaattg cagttaatga ggctattgcg gcaaatatgg aattattatc cacgcaacat
      601 ataagcttac tggatagaat aaaaggtaat agcgtaacat tattctgcga gctgatatta
      661 aatttcagaa atgaaataga gcgtagacaa ttaagcacaa acaccatgaa aagacacaac
      721 cagaggttga aaatcataag tgaatacttt ggtggtgttc cggttaaaaa tataggcata
      781 agggaggtgt acagctttct tgaaatcaga gcagctggga gtaaatttgc tattgctaac
      841 cagtacaggg cgcttttatc agacattttt aaaacagcta tagcttctgg tcttgccgaa
      901 gaagatccag cttctgcaac caggccattc agaactgaag tgaagcgcag tcgactgtta
      961 attgacgagt atctgttaat caggaaaata gcagacgtcc agaacgaat
//
LOCUS       DAZPLI010000386          985 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZPLI010000386 DAZPLI010000000
VERSION     DAZPLI010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.389"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            230..>985
                     /gene="gspA"
                     /locus_tag="ACHUU3_004557"
     CDS             230..>985
                     /gene="gspA"
                     /locus_tag="ACHUU3_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFR2084411.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIAITC"
BASE COUNT          280 a          199 c          237 g          269 t
ORIGIN      
        1 gcatctttat tatgattcgc cagatggaag gggaaacgta gtgtgggcac gatgtatgtt
       61 ctaataaaat agattgggtg ggttgtatat tgacgttagt ataaatagta ttaatgccgc
      121 ttgcatgaat caagcaaaat ttatttatat tagtaatatg tatttaatta ataaatacat
      181 acattatttt tatgattaag gataatcaat aattaaaaca ggaagttcta tgtctacgag
      241 aagagaagtt attctttcct ggttgcgtga gaaacgtcag acctggcgtc tgtgctattt
      301 gttgggtgag gctggaagtg gaaaaacctg gctggcgcag caactgcaaa aagataaaca
      361 tcgccgtgtg attactttaa gcctcgttgt ttcctggcaa ggtaaggccg catggatcgt
      421 taccgacgat aacgcggctg aacagggctg ccgtgacagc gcctggacgc gagatgagat
      481 ggcggggcaa ttactgcatg cgcttcatcg aactgatagt cgctgccctc ttattattat
      541 tgaaaatgct cacctgaatc accgccgcat acttgatgat ctccaacgcg cgatatctct
      601 tattcctgac ggtcaatttt tgctgatcgg cagacccgat cgaaaagtcg aacgtgattt
      661 taaaaaacag ggcattgaac ttgtctcgac aggacgtctg acggagcacg aacttaaggc
      721 aagcatcctt gaaggtcaga atattgacca acccgatctc cttttaacct ccagagttct
      781 gaaacggata gctttattat gtcggggcga tcgcagaaag ctggcgcttg ccggcgaaac
      841 aattcgctta ttacagcaag ccgagcagac cagcgttttc actgcaaaac aatggcgaat
      901 gatttaccgc atattgggtg ataaccgccc ccggaaaatg cagcttgccg tggtaatgag
      961 tggaaccatt atcgctatta cctgt
//
LOCUS       DAZPLI010000387          981 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZPLI010000387 DAZPLI010000000
VERSION     DAZPLI010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.390"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..107
                     /locus_tag="ACHUU3_004558"
     CDS             <1..107
                     /locus_tag="ACHUU3_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR2084412.1"
                     /translation="LETFRFYLEKQGWTLVNRGAIKNMTVEQLLKIHC"
     gene            118..306
                     /gene="dicB"
                     /locus_tag="ACHUU3_004559"
                     /gene_synonym="ftsT"
     CDS             118..306
                     /gene="dicB"
                     /locus_tag="ACHUU3_004559"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFR2084413.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            294..494
                     /locus_tag="ACHUU3_004560"
     CDS             294..494
                     /locus_tag="ACHUU3_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFR2084414.1"
                     /translation="MCTMNTVFALVLTVFLVSGEPVDMVTGVYSSMKECMTAAAKQKI
                     PGDCYPVDKVIHHDNNEIPAGL"
     gene            579..854
                     /locus_tag="ACHUU3_004561"
     CDS             579..854
                     /locus_tag="ACHUU3_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084415.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
BASE COUNT          327 a          181 c          211 g          262 t
ORIGIN      
        1 ccctggagac tttccggttt tatctggaaa aacagggatg gactcttgtt aaccggggcg
       61 caattaaaaa tatgactgtg gaacaattac tcaaaattca ttgttgagga cgcaataatg
      121 gagacgttat taccaaacgt taatacgtct gaaggttgtt ttgaaattgg tgtcactatc
      181 agtaaccctg tatttactga agatgccatt aacaagagaa aacacgaacg ggagctatta
      241 aataaaatat gcattgtttc aatgctggct cgtttacgtc tgatgccaaa aggatgtgca
      301 caatgaatac agtatttgca ctcgttctga cggtttttct tgtttccggt gaaccggttg
      361 acatggttac tggcgtatac agctcaatga aagaatgtat gactgccgca gcgaaacaga
      421 aaattcccgg tgactgttat ccggtcgata aagttattca ccatgataat aacgaaatcc
      481 cggcaggtct ttaaaacagt tccgtaataa atatccggtt tcattcttat atgccagcaa
      541 tggcaggggt tcgctcaacc ttaattaagg agaaaaacat gattaccagt tatgaagcca
      601 ctgttgtaac tactgatgac attgttcacg aggttaatct ggaaggaaag cgtattggct
      661 acgtgattaa gacagaaaat aaagaaaccc cattcactgt ggttgatatc gacggtccat
      721 caggcaacgt taaaacactt aacgatggtg taaaaaaaat gtgtctggtg cacataggaa
      781 agaatctgcc cgcagaaaaa aaagccgaat ttctggcaac tctgattgca atgaaattaa
      841 aaggtgaaat ctgaaaaaaa gaaagcctgc acactgtgca ggcctgagtg aagaacctgg
      901 gacatttatt catcactcgc agtaatttta atctgagttg aggttaaaaa acaatgagca
      961 ccgataaaca agtttaccca c
//
LOCUS       DAZPLI010000388          974 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZPLI010000388 DAZPLI010000000
VERSION     DAZPLI010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.391"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..950
                     /gene="tssI"
                     /locus_tag="ACHUU3_004562"
     CDS             <1..950
                     /gene="tssI"
                     /locus_tag="ACHUU3_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR2084416.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASELHGEQPQAVPGRSGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHVQKNMNTEVLNNRTTDVINNHAEKIGNN"
BASE COUNT          250 a          260 c          298 g          166 t
ORIGIN      
        1 tctgcatcag ccagttccgc tacagcgcac aaatccgtcc ttcttccgtg gtgaccaaag
       61 actacacctt taaacgaccc ggctgggcag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacacagtat gaagtgtatg actaccccgg acgtttcaag ggtgctcacg
      181 ggcagaactt tgcccgctgg cagatggatg gctggcgcaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttctccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcaagtga actgcacggc gagcagccgc
      361 aggcggtgcc gggacgcagt ggttcaggaa ccacgctgaa taaccacttt gcggtaatac
      421 cggcagaccg gacatggcgg ccacagccgt tgctgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggat tcggtaacct tgcgataccg cgcgtgggtc
      661 aggaggtcat tgtggacttc ctcaacggcg atccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggta gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgtgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgccg aaaaaatagg taacaactag gcgatcaccg
      961 ttaccaataa ccag
//
LOCUS       DAZPLI010000389          961 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZPLI010000389 DAZPLI010000000
VERSION     DAZPLI010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.392"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>961
                     /gene="htrE"
                     /locus_tag="ACHUU3_004563"
     CDS             <1..>961
                     /gene="htrE"
                     /locus_tag="ACHUU3_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFR2084417.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDS
                     FSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDVE
                     LKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNVG
                     QGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQ"
BASE COUNT          278 a          195 c          238 g          250 t
ORIGIN      
        1 ctgggcttcc ggacaaaatc gtagcaatta ctctattggc tacagtaaca gtgcatcctg
       61 gggcagctac agtgtcagtg cccagcgttc atggaatgaa gacggcgaca ctgacgatag
      121 cgtttatctc agtttcacca ttcctattga aaaattactt ggcactgaac aacggacttc
      181 aggtttccag agtattgata ctcaaatgag cagtgacttt aagggtaata accaactcaa
      241 cgtcagtagc agtggctata gtgataacgc tcgcgtcagt tatagcgtaa atactggcta
      301 tacgatgaat aaagccagca aagatttgag ttatgttggg ggttatgcca gctatgaatc
      361 accgtgggga accctggcag gttcagtttc tgcaaatagc gataacagtc gtcaagtgtc
      421 tctcagcacc gacggtggtt ttgtattgca tagcggcggc ctgaccttca gtaatgacag
      481 ttttagcgac tccgatacac tggcggtagt tcaggctcca ggtgctcaag gagcgcgaat
      541 aaattatggc aacagtacta tcgatcgatg gggttatggt gtcaccagcg ctctttctcc
      601 ttatcatgaa aaccgtatcg cgctggatat caacgatctt gagaacgatg ttgaattaaa
      661 aagtaccagt gcagtagctg taccgcgtca gggttcagtc gtctttgctg attttgaaac
      721 cgtgcaaggg caatcagcca ttatgaacat cacacgaagt gatggtaaaa atattccatt
      781 tgctgcagat atttatgatg agcaaggcaa tgtcattggt aatgttggac agggtggaca
      841 agcatttgtt cgtggcattg agcagcaggg aaatatcagc attaaatggc tcgaagaaag
      901 taaacccgta agttgtcttg cgcattatca acaaagttca gaagcagaaa aaatagcgca
      961 a
//
LOCUS       DAZPLI010000390          958 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZPLI010000390 DAZPLI010000000
VERSION     DAZPLI010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.393"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            6..958
                     /locus_tag="ACHUU3_004564"
                     /pseudo
     CDS             6..958
                     /locus_tag="ACHUU3_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
BASE COUNT          236 a          279 c          293 g          150 t
ORIGIN      
        1 cgtagggtga agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca
       61 cgcagagtca gtttgacgcc gtgggcaggc tcaggacaca gacggatgcc gcaggccgga
      121 caacagaata cagcccggat gtggtgacgg gcctcatcac gcgcatcacc acgccggatg
      181 gcagggcatc ggcgttttac tataaccacc acagccagtt aacgtcagcc accgggcctg
      241 acgggctgga aatgcgccgc aaatatgatg aatccggccg tctgattcag gaaaccgccc
      301 ctgacggcga tatcacccgc taccgttatg ataatccaca cagtgactta ccctgcgcaa
      361 cggaagatgc caccggcagc cggaaaacca tgacatggag ccgttacggt cagttgctga
      421 ccgtcactga ctgttccggt tatgtaaccc gttatgacca tgaccgtttt ggtcaggtga
      481 cggcggtgca ccgcgaggaa gggctgagcc agtaccgcgc atacgacagc cgtggacagt
      541 taattgcggt gaaagacacg cagggccatg aaatgcggta tgaatacaac gccgccggtg
      601 acctgaccgc cgtcattgcc ccggacggca gcagaaacgg gacacagtac gatgcgtggg
      661 gaaaagccat ctgtaccacg cagggcgggc tgacgcgcag tatggaatac gatgctgccg
      721 gacgggtcat ccgtctgacc agtgaaaacg gcagccacac caccttccgt tacgatgtac
      781 tcgaccggct gatacaggaa accggctttg acggccgcac acagcgttat caccacgacc
      841 tgaccggcaa acttatccgc agcgaggatg aggggctggt cacccactgg cactatgacg
      901 aagcagaccg cctcacgcac cgcaccgtga agggtgaaac cgcagagcgg tggcggta
//
LOCUS       DAZPLI010000391          955 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZPLI010000391 DAZPLI010000000
VERSION     DAZPLI010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.394"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            69..791
                     /locus_tag="ACHUU3_004565"
     CDS             69..791
                     /locus_tag="ACHUU3_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR2084418.1"
                     /translation="MLSGLLLLCLPFSSFACATTSHISFSYAARQRMQNRARLLKQYQ
                     THLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLS
                     SDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAKLPRTANE
                     MYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRL
                     AEPFWQDHFLGARRILTEETIL"
BASE COUNT          223 a          250 c          252 g          230 t
ORIGIN      
        1 gatacactgc aacatcaatg catgaccagc cattattgag aatgtctttg ccgtcgattc
       61 cctcttttgt gttgtcggga ttactgttgc tttgcttgcc gttttcttca tttgcctgcg
      121 ccaccacatc acatatctct ttcagctacg ccgcccgcca gcggatgcaa aaccgtgcgc
      181 gtttattaaa acagtaccaa acccatctga aaaaacaggc cagctacatt gtggaaggca
      241 atgcccaaag cagaagggcg ctgcgccagc acaaccggga gcagataaaa cagcatccag
      301 aatggtttcc tgcgccgctc aaggcgagtg acagacgctg gcaggcgctg gcggaaaaca
      361 accacttttt aagcagcgat cacctgcata acatcaccga agtggcgatt caccgtctgg
      421 agcagcagct tggcaagcct tacgtctggg gcggcacgcg gcccgatcaa ggcttcgatt
      481 gtagtgggtt agttttttat gcctacaaca agatcctcga ggcgaagctc ccgcgtacgg
      541 ccaatgagat gtatcactac cgtcgggcaa ccattgtggc gaataacgac ctgcgccggg
      601 gagatttgct gtttttccat atccacagcc gcgagattgc cgaccatatg ggggtgtatc
      661 tgggcgacgg tcagtttatt gagtcaccgc gcaccgggga aaccattcgg gtaagtcggt
      721 tagctgaacc tttctggcag gatcattttt tgggcgcgcg gcggatattg acggaagaga
      781 cgattttgta ggacggataa ggcgtttacg ccgcatccgg cggtggtgcg cagatgcctg
      841 atgcgacgct gacgcgtctt atcatgccta caaatctgtg cgtttttgcg agccgctttc
      901 ctgttattcc atcacccagg gaaagatcat cagaagtaca ctttttgtac taaat
//
LOCUS       DAZPLI010000392          936 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZPLI010000392 DAZPLI010000000
VERSION     DAZPLI010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.395"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(198..>936)
                     /locus_tag="ACHUU3_004566"
     CDS             complement(198..>936)
                     /locus_tag="ACHUU3_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR2084419.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          280 a          198 c          199 g          259 t
ORIGIN      
        1 attgcttctc cgataaaatg tcagagaagc atgattacgt ggttattgtc tgttgatgac
       61 ttgttgaccg agccgtcatc attctcaatg cattagttaa atcatttgtg atttttaagg
      121 attatcctgt ctatgatttt ctacttagaa ggatatggca ctaactatgc atcttatagt
      181 ttactgctgg catctattta aaatagcaat tgagtcaaat agcccatcaa gatagccata
      241 cctaatataa ccgtcaggaa tgcagctatc atcggcattc tgaacattga tttaagtaat
      301 atcacttccg tcaggcttgc gccggcactg ccgatgatta atgccattaa tgctcccaga
      361 cccattcctt ttgtcatcag aacagatgcc agagggataa ccgcctcagc ccggatatac
      421 agcggaatac cgacaacggc gcttaatggg atggcgaagg gattatctgc tcctgcatga
      481 gcggcaatcc acgctgaggg aataaagcca taaataaaag atcctattaa tactcctaac
      541 aaaagatatg gcagtacatc tttaaactgt tgcaacgcat ctttggtggc tagttttatg
      601 ggattaatac ttactacagc tttggtactg caacaggtaa ctacggggtt ctcaatggct
      661 ttagccgctg gactacaaca actcactttt atgggcgtat atgtcgttcc cggcgaagtt
      721 tttgctggag cacaacaatt tgctgatgag cttttactgg caatgatatg acgttcaaac
      781 cccagagaat ccaggataat actggcaaga acggagacgc cggctgcgat aatcgcgtac
      841 aacaaggtga ctttccaacc aaaggtcatc cacattagtc cgacgataat gggattaagt
      901 aatggtgaaa caaataaaaa agtgagagtc ggacca
//
LOCUS       DAZPLI010000393          925 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZPLI010000393 DAZPLI010000000
VERSION     DAZPLI010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.396"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(345..>925)
                     /locus_tag="ACHUU3_004567"
     CDS             complement(345..>925)
                     /locus_tag="ACHUU3_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR2084420.1"
                     /translation="RLYLAEMLYYPIDLIEKFQNLYAITDQIRNTTGFCLPQNSELIY
                     CWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSKQSLISRCYNLMLSEP
                     GTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSAIQTTVKPISEIA
                     RENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          282 a          184 c          165 g          294 t
ORIGIN      
        1 atcttaacgt ataaactaga tttaataata acgaactaga tttaataaca acgatatccg
       61 tacctatccg gatatctaat gtcataagca tccctttaat tatcccctca acctcttgta
      121 actttcccac tcttgaactc cacatagtcc aaacgccgga tgcaaaaaaa tcacacgtgc
      181 atttctgaaa ttgtgatgga ggaagatagt atcaattata tgtgcttata aacgtccccg
      241 attagcgcag aagtcaccgg attgttcagg ctccaatagg ttgagtatgc cgtatttatt
      301 ttccaaaata aaaatcgatg ctgttgctcc ttcataaaat acttttactc tctggacgct
      361 tttcttagct ctcttggtgt tatcttaaaa cgattatgga acctttcagt aaaacgagaa
      421 ggacacttgt aaccattttc cctggcaatc tcgctgatgg gttttactgt cgtttgtata
      481 gcagacaacg cattatttaa cctcacgtcg tccaatatac tttgaaaact tactccctcg
      541 cttgccagac ggcgatgcaa tgtggaaaca gaaatgtaga gatatctcgc taccttgttt
      601 gctgtccatt ttgttccagg ttcggatagc attaggttat aacaacgact tattaatgat
      661 tgtttactat ctgataaaag taaacaatta acatgatgtg ctcctaatga aagtagaacg
      721 cccattgcta agtgttcctg aatttgcgtt gagaagcctc gggaaataga tgtttttagt
      781 tgctcccaac aatatattaa ctcggaattc tgaggtaagc agaaacctgt cgtgttacga
      841 atttgatcgg ttatcgcata aagattttgg aacttttcaa ttagatcaat gggataataa
      901 agcatttctg caagataaag acggt
//
LOCUS       DAZPLI010000394          923 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZPLI010000394 DAZPLI010000000
VERSION     DAZPLI010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.397"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..347)
                     /locus_tag="ACHUU3_004568"
     CDS             complement(<1..347)
                     /locus_tag="ACHUU3_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFR2084421.1"
                     /translation="MNNCIFFSISASTVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQL"
     gene            complement(364..>923)
                     /locus_tag="ACHUU3_004569"
     CDS             complement(364..>923)
                     /locus_tag="ACHUU3_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR2084422.1"
                     /translation="RCKTRWTRCTRRREKIGLKGGWNFYQYPLNPITDIDPLGLATCL
                     YSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNNMQCKNNPGCTHLQNRGPIPQGV
                     WSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLTQSCLNAFGPSLGPRFCSEGCIT
                     GSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          265 a          189 c          164 g          305 t
ORIGIN      
        1 agttggagtg gatttagatt tttcagatat ggtcttatga tctttccctt acttaataaa
       61 tagcatgtat aggattcgga aagtccagca tctattttgt acctcattat attagataag
      121 gatgtgagaa attccttgtt atcattcgca gccatatatg ccagtccgag ttcagcctct
      181 gcggccttac aggcaaaata gcttagtgaa cacgcttttc gtccctcttt agtactggct
      241 gatattagtg cccataatat tgctttgtct gccaaatcta actctctctg aaattgtctt
      301 gcagtaactg tactcgcaga aatagagaaa aaaatacaat tgttaataac tttgccatat
      361 tttttagtcc gtaactgtta atgtgctatc tggctctgaa aatattaact catttaattt
      421 ttgcatgtca ttagatgaac cagttataca accctcggaa cagaatcgag gtcctaaaga
      481 cggaccgaaa gcgtttaaac aactttgtgt taaaaatcca tcacgattat atgtttctgt
      541 atttgctgac ggtactaaac gtataccatt aggttttcta ttggttgctc caggaccatt
      601 aacattccag ctccataccc cttgaggtat tggacctcta ttttgtaagt gtgtacaacc
      661 aggattattt ttacattgca tattattacc attattacca gaggcgacag gaatagtaag
      721 cacatcataa gagttatcat cattttgcgt atctgaaacg catgaaagca tacttaaagt
      781 tatgctgtat aaacaggtcg ctaatcccaa cgggtcaata tcagttatcg gattcaacgg
      841 atactgataa aaattccatc cccccttcag tccaatcttt tcgcgccggc gtgtacaccg
      901 ggtccatctg gttttgcatc tgc
//
LOCUS       DAZPLI010000395          917 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZPLI010000395 DAZPLI010000000
VERSION     DAZPLI010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.398"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..144
                     /locus_tag="ACHUU3_004570"
     CDS             <1..144
                     /locus_tag="ACHUU3_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR2084423.1"
                     /translation="PLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTFYTP
                     QGS"
     gene            complement(168..917)
                     /locus_tag="ACHUU3_004571"
                     /pseudo
     CDS             complement(168..917)
                     /locus_tag="ACHUU3_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT          239 a          251 c          204 g          223 t
ORIGIN      
        1 ccgctctcca gggcttacta cacccgcaaa atgagtcagg gaaaacgaca caatcaggcg
       61 cttatcgccc tggcgagacg acgctgcgac gttctgttcg ccatgatgcg cgacgggact
      121 ttttataccc cgcaggggtc ataacatgct tgacaactta ataggggccc cccccacaaa
      181 aaggcaaaca gatccgaagc gggataatct ttttggggag aattgttcca gttagcaata
      241 tatcccgaca gggggttata caccttaggg ttcatttcaa aaggcaatag ccctttccag
      301 tcccattttc ccgtaccagg aacgggtaat cgcggatcat ggcctgattg acgatctgga
      361 taagcaccag tatgaacata accaatattg ccgtttacat cagcatagta ccagttgatg
      421 gtcagtgctt gtttcgctgc ctgctgtgtc cactcctgcc aatttttggc cttcatctga
      481 tgagtccagg ccagcaaaga cgccacctct ttaccatccc atgcgcggga tttagcgtaa
      541 gccgtttgtg tcgtctggtc agtttggaga atgttgccat gcaccgtacg ccagacagta
      601 aaggtctctg cctgaccatt tttcaccgta atggtttcct cacggcttaa cattttcacc
      661 cacttaccat tatgcaagta gtagcctggt ttctctgccg acagccgttc agcaaaaata
      721 tcgacatcat cgccgaaacc tgccgttgat ccccaggaaa tcacaccatt atgaccaaaa
      781 accagcccag gataggatag gataggcaaa tggtgtattg ccagtgacat cataaccagc
      841 accgtgcaga ccaataccat aagtatacgc aggcgcatac cagccaaact gcggaccatt
      901 taccatgatt gctttcg
//
LOCUS       DAZPLI010000396          911 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZPLI010000396 DAZPLI010000000
VERSION     DAZPLI010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.399"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..761
                     /gene="ydeO"
                     /locus_tag="ACHUU3_004572"
     CDS             <1..761
                     /gene="ydeO"
                     /locus_tag="ACHUU3_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR2084424.1"
                     /translation="SLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYE
                     RHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNH
                     HNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLY
                     ISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAF
                     RKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
BASE COUNT          272 a          181 c          180 g          278 t
ORIGIN      
        1 tgtcgctcgt ttgttctgtt atatttattc atcatgcctt caacgctaac attttagata
       61 aagattacgc cttctctgac ggcgagatcc tgatggtaga taacgctgtt cgtacgcatt
      121 ttgaacctta tgagcggcat tttaaagaga tcggatttac tgaaaatacc attaaaaaat
      181 atctacaatg cactaacatc cagacagtga cggtgcctgt tcctgcgaag tttttacgtg
      241 cttcaaatgt accgactgga ttgcttaatg aaatgattgc ttatcttaac tcggaagaac
      301 gcaatcatca taatttttca gaacttttgc ttttttcttg cctgtctatt tttgccgcat
      361 gcaaaggttt cattacacta ttaactaacg gtgtgctatc cgtttccgga aaagtgagaa
      421 atattgtcaa catgaagctg gcgcacccat ggaagctgaa agatatttgt gactgcctgt
      481 acatcagtga aagcctgttg aagaaaaaac taaagcaaga gcaaacgaca ttctcacaga
      541 ttctattaga tgccagaatg cagcacgcaa aaaatttgat acgcgtagaa ggttcagtca
      601 ataaaattgc cgaacaatgt ggttatgcca gtacatctta ttttatttat gcgttccgca
      661 aacatttcgg caacagtccg aagagagttt ctaaggagta ccgttgtcaa agtcacatgg
      721 gtatgaatac gggcaacacg atgagtgctt tagctatttg attatttgct aacgagtagt
      781 caaccacgca cgctgcgtaa gaattaaatg gggcagccat tccctgcccc gcgttgtttt
      841 taggcgatat atttattgaa ataaataagt gacatccatc acatatttat gcacttgcat
      901 aacctgttgc a
//
LOCUS       DAZPLI010000397          907 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZPLI010000397 DAZPLI010000000
VERSION     DAZPLI010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.400"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..796
                     /locus_tag="ACHUU3_004573"
     CDS             <1..796
                     /locus_tag="ACHUU3_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR2084425.1"
                     /translation="HDNETELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVAS
                     DVNDPATTCTCALYGFQFQGAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQS
                     KNSQTSSLWDAFAYTLAILYYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITL
                     EQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGY
                     ENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(839..>907)
                     /locus_tag="ACHUU3_004574"
     CDS             complement(839..>907)
                     /locus_tag="ACHUU3_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084426.1"
                     /translation="GGTDEIMIYVAGRQILKDYQNK"
BASE COUNT          259 a          184 c          197 g          267 t
ORIGIN      
        1 tcatgacaat gaaaccgagt tgatttacgt caagaaaggg gtagcaaggt ttaccatcga
       61 ttcttcgtta tatgtcgcgc acgccgatga cattgtggtg atagaacgtg gcaggctgca
      121 tgcggtggcc tctgacgtta acgatccagc aacgacgtgt acctgtgcgc tgtacggctt
      181 tcagtttcag ggggctgagg aaaatcagct actgcaaccg cattcttgcc cggtaattgc
      241 cgcagggcag ggaaaagaag tcattaaaac cttatttaat gagctaagtg tgattttgcc
      301 gcaaagtaaa aatagccaaa catcttcgtt atgggatgca tttgcctata cattagcaat
      361 tctttactac gaaaacttta aaaatgctta tcgttcggag caggggtata ttaaaaaaga
      421 tgttctgata aaagatattc ttttctatct gaataataat tatcgcgaaa aaatcacttt
      481 agaacagtta tcgaaaaaat ttcgtgccag cgtcagttat atttgccatg aatttaccaa
      541 agagtatcgt atttccccta ttaactatgt tattcaacgg cgtatgacgg aagcaaaatg
      601 gtcactcact aatactgaat tatcacaggc agagatctcc tggcgtgtgg gttatgaaaa
      661 tgtcgatcac tttgccaaac tgtttttgcg ccatgtcggt tgttcgccca gcgattaccg
      721 taggcaattt aaaaattgtt ttgcggaaca agaaatctta tctgaatttc ctcaaccggt
      781 aagtcttgcc ggataaatct ggcggcgcac tttgttaaaa agctgcgccg cctgcagatt
      841 atttgttctg atagtctttc aggatctgcc gacctgctac gtaaatcata atttcgtctg
      901 taccgcc
//
LOCUS       DAZPLI010000398          906 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZPLI010000398 DAZPLI010000000
VERSION     DAZPLI010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.401"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="ACHUU3_004575"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="ACHUU3_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR2084427.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITYIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          267 a          144 c          222 g          273 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tatgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtga
//
LOCUS       DAZPLI010000399          901 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZPLI010000399 DAZPLI010000000
VERSION     DAZPLI010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.402"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..373
                     /locus_tag="ACHUU3_004576"
     CDS             <1..373
                     /locus_tag="ACHUU3_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084428.1"
                     /translation="NKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKATL
                     NRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSPDS
                     EEWSSQKASKDVEGIVFGGKK"
     gene            370..798
                     /locus_tag="ACHUU3_004577"
     CDS             370..798
                     /locus_tag="ACHUU3_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084429.1"
                     /translation="MINITTKDGTQVSGEVRDLVILLETDGKTVSDYLQPIPPKKDTH
                     WSMIFIPVAIYILLNLVYIIAHPLFPEKYLKTSTSIFLFISFILSAIVAYFVFHKHKS
                     ATLSICSLVFLVLIVGANVGFISYSDLTQKATQKLDSLSK"
BASE COUNT          309 a          148 c          159 g          285 t
ORIGIN      
        1 caacaaagaa aacaaagata aatatattaa caagttcaag aaagagcttt caaaatatat
       61 acaaccaact gctggagtgg acattcaatt atttaactct aaggatggtg gtggtgtaat
      121 taaagccact ttgaatagaa gtggaaaaag aaaatcatct attgctggta attttactaa
      181 attaggtgag gcaattacct cgagtgggca gagggttttt ggtggtgatc tatcaaatgt
      241 gaatttttac ggaacaaata cgatttttga tggagataca atatttttaa ttaaatcacc
      301 tgattcagag gagtggtcat cacaaaaagc ttccaaagac gtagagggca tcgtatttgg
      361 agggaaaaaa tgattaacat tactactaag gatggcactc aagtcagtgg tgaagtaaga
      421 gatctagtaa ttcttcttga aactgatgga aaaacggtgt ctgattattt gcagccaatc
      481 cctcccaaaa aagatacaca ctggagcatg attttcattc cggtagcaat ttatatacta
      541 cttaatcttg tatacattat tgctcaccca ttatttcctg aaaaatatct aaaaacatca
      601 acatctatat ttctattcat ttccttcata ttaagtgcaa tagtagcata ttttgttttc
      661 cataagcata aatctgccac tctatccata tgcagcttag tgttcttagt gttaattgta
      721 ggtgctaacg tagggtttat cagttacagc gacctaacac aaaaagcgac gcaaaaatta
      781 gatagtttgt ctaaataatt atgttgccat gcgaagtatg catggctgca tgtctgcctt
      841 ccatttaaaa tgggctaaga cctataaccc taaatattat tctttattat cttctttacc
      901 a
//
LOCUS       DAZPLI010000400          890 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZPLI010000400 DAZPLI010000000
VERSION     DAZPLI010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.403"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..570)
                     /locus_tag="ACHUU3_004578"
     CDS             complement(<1..570)
                     /locus_tag="ACHUU3_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFR2084430.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLA"
     gene            complement(570..>890)
                     /locus_tag="ACHUU3_004579"
     CDS             complement(570..>890)
                     /locus_tag="ACHUU3_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR2084431.1"
                     /translation="AAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAGSVI
                     VTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLKQPA
                     QEAS"
BASE COUNT          297 a          211 c          194 g          188 t
ORIGIN      
        1 cgcaagaccc attaagatcc ctggcaaata tcccgccata aatagcgatg ctacagaaac
       61 gccgccagct gtcagggaaa acacaattaa cacgtttgag ggcggtatca gtaatccagt
      121 aatacaagat gagacgttta cagcggtaga aaatgccgga tcataacctt gtttggtctg
      181 aatgggattc agtgtgccac caacggcagc agctgccgca acggctgaac cagaaatgga
      241 accaaacatc atattcgcca ggacgttcac atgaccaagt gaaccaggaa cccgaccaac
      301 cattacctgg gcgaggttaa tcaaacgtat cgcaataccg ccgctgttca tcagcgttcc
      361 ggcaaagatg aaaaagggga ttgccaatag ggcaaagtta tccagtccat tagccagtcg
      421 ctgcgaaaca gtgattgccg cgatatccca gggaaacatc aaaagcattg aagctacagt
      481 ggcaataccg attgagaacg aaatgggcac accaataaag accagcacaa aaaaacagcc
      541 aaacagtgta atcgcgatat aactatccat taactggcct cctgcgcagg ttgtttcagt
      601 tgttgaatgc tttgattaat aaaacacaag gcataaaaca tcatcaccaa tccacttaat
      661 ggcagaatga tatatacgta tcccatcggt atttgcatcg cggcagatac ctgtgatgtt
      721 gccagcgttt tatcaattaa tttcaggcca ccagtcacaa tgaccgaacc agcaaaacct
      781 aaaataagaa cgttaatgac tatactaaga agtaattgct tgcgcttatt cagggcgaga
      841 gcaaataaat cgatagagag atgtctctgc gcgccgacgg tatatgcagc
//
LOCUS       DAZPLI010000401          890 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZPLI010000401 DAZPLI010000000
VERSION     DAZPLI010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.404"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..207
                     /locus_tag="ACHUU3_004580"
     CDS             <1..207
                     /locus_tag="ACHUU3_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084432.1"
                     /translation="DAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNALWIELEGV
                     EYAFPDPQTQNETQQAGYDENIPV"
     gene            258..446
                     /locus_tag="ACHUU3_004581"
     CDS             258..446
                     /locus_tag="ACHUU3_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFR2084433.1"
                     /translation="MYKITATIEKEGGSPASWTRYSKKKLTKSECEKMLSGEKEAGAS
                     REQKVKLINFNCEKLQSS"
     gene            553..813
                     /locus_tag="ACHUU3_004582"
     CDS             553..813
                     /locus_tag="ACHUU3_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR2084434.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMGDAANLLI"
BASE COUNT          292 a          180 c          205 g          213 t
ORIGIN      
        1 gacgccaaac gcgcaaaagc tgatttgctg gaaaaaacat tcacctcccg ctcatgggaa
       61 agaatcaaag gtatggactg gccgacaatc aaagctggtc gtaatgcact atggattgaa
      121 ctggaaggcg ttgaatatgc attcccggat ccacaaacac aaaacgaaac gcaacaagca
      181 ggatacgatg agaatattcc ggtgtaaaac caagtgcatt ttggacacgg tttttgaaaa
      241 tattaatgga ggtgttcatg tataaaatta cagccactat tgaaaaggaa ggtggttctc
      301 ctgccagctg gacaagatac tcaaagaaaa aactaacgaa atcagaatgc gaaaaaatgc
      361 tctcagggga aaaagaagca ggcgcttcca gagagcagaa agtaaaactg ataaatttta
      421 attgcgagaa acttcagtcc tcgtgaattt cattatatcc agattagaac ttcatagctg
      481 attattaaaa atcaaccaca cccgccagta ttctgtatat ttactggcgg tcatatcgtg
      541 agaggtatgg caatgaatct tgtgacactc aaaacgtggg gaaaactcag atatccggat
      601 aacccaccat caatatcaac gctgagacga tgggcaagga atggaaacat ttatcctgca
      661 cctgaactac acgggaggag ttacagggtg gttccggagg ctttctatat caacccaaat
      721 aaggttgata ccgatataac acaccatcag cctaatgggc gacaagggag agacagtccg
      781 ttaatgggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc
      841 agtggcttct gtttctatca gctgtccctc ctgttcagct actgacgggg
//
LOCUS       DAZPLI010000402          887 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZPLI010000402 DAZPLI010000000
VERSION     DAZPLI010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.405"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            59..859
                     /locus_tag="ACHUU3_004583"
                     /pseudo
     CDS             59..859
                     /locus_tag="ACHUU3_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          255 a          171 c          214 g          247 t
ORIGIN      
        1 ttggataacg gcacttattg atatattcat gaagattata atcacaaggg aatacataat
       61 gaaaagaaaa gttctggcaa tgctggtccc agcgttatta gttgctggcg cagcaaatgc
      121 ggctgaaatt tataataaaa atggcaataa actggatctg tacggaaaag ttgatgctcg
      181 tcatactttt tccgataacc ctggcgatga cggcgatgaa actatcatcg agttaggctt
      241 caaaggtgag acccaaatta cagatcagtt aactggttat ggtcaagcac tgaccaaaac
      301 aaaggctagc gacactgaag gttctgataa tacttatgtc aaacttgctt ttgcaggtct
      361 gaagttcggc gagatgggct cctttgacta tggtcgtaac tatggtgtga tttacgacgt
      421 tgaagcctgg acagatatgc ttcctgtatt cggcggcgac tcctatacct ggactgataa
      481 cttcatggca ggccgtgcaa atggggtagc tacttaccgc aatagtgatt tcttcggtct
      541 ggtggaaggt ctgaactttg cattgcagta tcaaggtaac aacgaaggca gcaatgcagg
      601 agaagatcaa gaaggtacta aaaatggtca cgaagacgtt cgcttccaga acggtgatgg
      661 ttttggtctt tccacttcat atgactttga cttcggtttg agcttaggtg ccgcttactc
      721 aaactctgac cgtaccaatt ctcaagtagc tcttggcggg tatcactaca atgaatacag
      781 caaatttgct ggtggtgata cagctgaagc atggacattt ggtgcaaaat acgacgccaa
      841 caatgtttat cttgcaatgg gtgatgctgc caacttactg atttagt
//
LOCUS       DAZPLI010000403          877 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZPLI010000403 DAZPLI010000000
VERSION     DAZPLI010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.406"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>877
                     /gene="cadA"
                     /locus_tag="ACHUU3_004584"
     CDS             <1..>877
                     /gene="cadA"
                     /locus_tag="ACHUU3_004584"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR2084435.1"
                     /translation="NFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVK
                     GDVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEI
                     KRLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPG
                     MEKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTD
                     FKRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVL
                     PTMVMTPYAAFQKELH"
BASE COUNT          238 a          221 c          212 g          206 t
ORIGIN      
        1 caacttctca ccgatttacg aaggtaaatg cggtatgagc ggtggccgtg tagaagggaa
       61 agtgatttac gaaacccagt ccactcacaa actgctggcg gcgttctctc aggcttccat
      121 gatccacgtt aaaggtgacg taaacgaaga aacctttaac gaagcctaca tgatgcacac
      181 caccacttct cctcactacg gtatcgtggc gtccactgaa accgctgcgg cgatgatgaa
      241 aggcaatgca ggtaaacgtt tgatcaacgg ttccattgaa cgtgcgatca aattccgtaa
      301 agagatcaaa cgtctgagaa cggaatctga tggctggttc tttgatgtat ggcagccgga
      361 tcatatcgat acgactgaat gctggccgct gcgttctgac agcacctggc acggcttcaa
      421 aaacatcgat aacgagcaca tgtatcttga cccgatcaaa gtcaccctgc tgactccggg
      481 gatggaaaaa gacggcacca tgagcaactt tggtattccg gccagcatcg tggcgaaata
      541 cctcgacgaa catggcatcg ttgttgagaa aaccggtccg tataacctgc tgttcctgtt
      601 cagcatcggt atcgataaga ccaaagcact gagcctgctg cgtgctctga ctgacttcaa
      661 acgtgcgttc gacctgaacc tgcgtgtgaa aaacatgctg ccgtctctgt atcgtgaaga
      721 tcctgaattc tatgaaaaca tgcgtattca ggaactggct cagaatatcc acaaactgat
      781 tgttcaccac aatctgccgg atctgatgta tcgcgcattt gaagtgctgc cgactatggt
      841 aatgactccg tatgctgcgt tccagaaaga gctgcac
//
LOCUS       DAZPLI010000404          866 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZPLI010000404 DAZPLI010000000
VERSION     DAZPLI010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.407"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>866
                     /locus_tag="ACHUU3_004585"
     CDS             <1..>866
                     /locus_tag="ACHUU3_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR2084436.1"
                     /translation="IPVHDTIARVVSCISPAKFHECFINWMRDCHSTDDKDVIAIDGK
                     TLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKII
                     TTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEKKFPLTELNNPAHDSSAMSE
                     KSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMMVRYYI
                     SSADLTAEKFATAIRNHWHVENNLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINV
                     LTNDKVFKAGLRR"
BASE COUNT          284 a          157 c          207 g          218 t
ORIGIN      
        1 attcctgttc acgataccat tgccagagtt gtatcctgta tcagtcctgc aaaatttcac
       61 gagtgcttta ttaactggat gcgtgactgc cattctacag atgataaaga cgtcattgca
      121 attgatggaa aaacgctccg gcactcttat gacaagagtc gccgcagggg agcgattcat
      181 gtcattagtg cgttctcaac aatgcacagt ctggtcatcg gacagatcaa gacggatgag
      241 aaatctaatg agattacagc tatcccagaa cttcttaaca tgctggatat taaaggaaaa
      301 atcatcacaa ctgatgcgat gggttgccag aaagatattg cagagaagat acaaaaacag
      361 ggaggtgatt atttattcgc ggtaaaagga aaccaggggc ggctaaataa agcctttgag
      421 aaaaaatttc cgctgacaga attaaataat ccagcgcatg acagttccgc aatgagtgaa
      481 aagagtcacg gcagagaaga aatccgtctt catattgttt gcgatgtccc tgatgaactt
      541 attgatttca cgtttgaatg gaaagggctg aaaaaattat gcgtggcagt ctcctttcgc
      601 tcaataatag cagagcaaaa gaaagagccc gaaatgatgg tcagatatta tatcagttct
      661 gctgatttaa ccgctgagaa gtttgcgaca gcgattcgaa atcactggca cgtggagaat
      721 aatctgcact ggcgtctgga cgtggtaatg aatgaagacg actgcaaaat aagaagagga
      781 aatgcagcag aattattttc agggatacgg cacattgcta ttaatgtttt gacgaatgat
      841 aaggtattca aggcagggtt aagacg
//
LOCUS       DAZPLI010000405          858 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZPLI010000405 DAZPLI010000000
VERSION     DAZPLI010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.408"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            1..858
                     /locus_tag="ACHUU3_004586"
                     /pseudo
     CDS             1..858
                     /locus_tag="ACHUU3_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          164 a          288 c          230 g          176 t
ORIGIN      
        1 ctggttgttg atcagccagc taccatcggt gcgttacctg tcgccgttgc ccgctcagaa
       61 ggagtccttg tcggatacct ccctggactg gccatgcgcc gcatagccga cttacacgcc
      121 ggtgaagcta aaactgatgc tcgtgacgct gccatcattg ccgaagctgc ccgtaccctg
      181 cctcacgcgc tacgcacgct gaaactggct gacgagcaaa tcgccgaact ctccatgctc
      241 tgcggcttcg atgatgatct tgccgcacag acaacgcagg ccagcaaccg tatccgcggc
      301 cttctgaccc agatacatcc ggcactggag cgcgttctcg gtccgagact tgagcacccg
      361 gcggtactcg atcttctcca gcgatatccc tcaccagaaa aactcgcttc gctgggcttc
      421 gctgggtgag aagaagctgg cagcccagct ctgcaaactt gcgcctcgtc tgggtaaacg
      481 ccttgcagca gacatagctc aggcactggc cgaacaaacc gtcgtcgttc ccggcacgaa
      541 tgccgctgcc gtagtactgc cacgtctggc actccagctc atcacgctgc gtaagcaaag
      601 agacgaggtg gcgcttgagg tagaacagcg agttcttgct caccctcttt acccggtcct
      661 gaccagtatg cccggagtcg gtgtcaggac cgcagccaga ctcctcaccg aggtcgcctg
      721 ccgcgccttc gcctctgccg cacatctcgc tgcttatgct ggccttgcgc cggtaactcg
      781 gcgatccggc tcgtcaatac gcggtgagca tccctcgcga cggggtaata aagctctcaa
      841 acgggcgttg ttcctgtc
//
LOCUS       DAZPLI010000406          838 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZPLI010000406 DAZPLI010000000
VERSION     DAZPLI010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.409"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            1..830
                     /locus_tag="ACHUU3_004587"
                     /pseudo
     CDS             1..830
                     /locus_tag="ACHUU3_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          320 a          143 c          156 g          219 t
ORIGIN      
        1 tccaatgata cagaccatat tactattggt atcgacaacc aagaaccaat agaattacct
       61 gcgaactata aagacaaaga actcgtccgt actatcatta atgacaacat agttgagaag
      121 actcatgata tcaataacaa ggaaatgatc ttcagcgcat taaaagaaat atatgatgga
      181 gatcctggtt ttatcttcga taagatatca cacaaactca gacatacggt aacggaattt
      241 gatgagagcg ggaatagcga accaacggac ttatttacct ggtacggtaa agataaaaaa
      301 ggcaactctc tcgctattgt aattaaaaat aaaaacggaa atgattactt atctctcggt
      361 tactacgatc aggacgacta ccacattcaa agaggaattc gtattaatgg tgatagtctc
      421 acccaatatt gtagtgaaaa agccaggaat gcttcagcaa tgcttcagcg tggtttgaaa
      481 gcagtaaagc tatcatggca gaatcatttg caacaggttc cgatcatcag gttgtaaacg
      541 agctcaacgg ggaaagactg agagaaccta acgaagtttt taaacgttta ggtcgagcaa
      601 taagatataa ttttcaagtg gacgatgcaa aatttagacg cgacaatgta aaagaaataa
      661 tttctaattt attcgctaac aaagttgacg ttgaccatcc tgaaaataaa tacaaagact
      721 ttaaggatct cgaagataac gttgaaaaaa gacttcaaaa tcgccaggcg aaatatcaaa
      781 atgaaattaa tcaactatct gcgccaggtg ttaattttga tgatatttaa aattattt
//
LOCUS       DAZPLI010000407          822 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZPLI010000407 DAZPLI010000000
VERSION     DAZPLI010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.410"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..304)
                     /locus_tag="ACHUU3_004588"
     CDS             complement(<1..304)
                     /locus_tag="ACHUU3_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR2084437.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
BASE COUNT          263 a          181 c          175 g          203 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 ccgtcaggta ggccattttg tccagcacct gcgcaaattc aagggagaga aactgctggc
      181 tgaccagaga gagatcgagg gaaaagagcg aagagagtga ctggttcaaa tgaaaggaaa
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag tgtgaaacgt aatccggttg
      301 acatagcaac ctccgtgaaa atgaataata atccgcacat attgcatgta caacatgtcg
      361 ggcggatgag ataagcgagt cgacatctgg cgttaccggg gaatatcaga aaaatgtcat
      421 ggttatgtga tgatttagtc tgggatttta cttcagagaa acgggtgtct gtttagtcaa
      481 caaaataatc acaaacgcaa cggatgggtt ctcgacgtta ttcacgacag gctcgtacat
      541 tacagcgagt aataaaaaac ctgatatgca aagtcaaacc aatattcata atgtcttttt
      601 attcacaaca ataaataatt caataacaat cattgaaatg aatgatggtt tgtataagga
      661 atatacgcta ccgcccaccc tgataacatg atctgcacat tgccatctta acgtcgttaa
      721 acactggctt aatagtggat atttcaaatt aggaatttat taaattcacc tgatgaatat
      781 aataagcaaa tagcatagaa aataatggca actccccccg ag
//
LOCUS       DAZPLI010000408          817 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZPLI010000408 DAZPLI010000000
VERSION     DAZPLI010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.411"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(246..>817)
                     /locus_tag="ACHUU3_004589"
     CDS             complement(246..>817)
                     /locus_tag="ACHUU3_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR2084438.1"
                     /translation="FFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIV
                     IAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFF
                     IAVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQ
                     IHLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          260 a          203 c          174 g          180 t
ORIGIN      
        1 agggacagct gatagaaaca gaagccactg gagcacctca aaaacaccat catacactaa
       61 atcagtaagt tggcagcatc acccgctttg gcttgataac cggataacaa cgtgcgcttt
      121 gttgatgccg gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc
      181 attacattaa gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt
      241 ccacgtcagg ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg
      301 ggcggtactt tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca
      361 ttgaggcgat agaaacggaa gtagagaatc agcgttacca tgaaaaacag agttggtaga
      421 gcaatcataa taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca
      481 tagccaatca tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc
      541 accaccatag tctgcacgga gtaagcgata ctttcacagc gtacgtgcag tttatattca
      601 ccgtaatcaa cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc
      661 acattcagca aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc
      721 attaacagga gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg
      781 cgtcgggata atgatttaac taagcgtggg aagaata
//
LOCUS       DAZPLI010000409          799 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZPLI010000409 DAZPLI010000000
VERSION     DAZPLI010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.412"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..741)
                     /locus_tag="ACHUU3_004590"
     CDS             complement(<1..741)
                     /locus_tag="ACHUU3_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFR2084439.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          268 a          137 c          161 g          233 t
ORIGIN      
        1 acataaacag gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa atacagcaat
       61 ggaaaaaatt ttatcggaga acaggaataa atctaaaaga tagattgctg aaatacagac
      121 tccactttta catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa
      181 catcactgca aatacccaaa aaatagcaaa gtaataatga tcactggaag acgctgattc
      241 actttctgaa ggattagtcg gatattggtg ttgtgaagtg agttggttat tcaaagcgtt
      301 tatagctagc tctaatccct gagctaattt ttgttgttta aatgcgggta tcatgttgct
      361 acggatgata tctccagcca gagcatcggt taccttttct tccagcccat aacctacctg
      421 gatgcggaca gtacgatctg accaggcaac aatgatcagt atcccatcat tacgtttggc
      481 atctcctaaa cgccaattgt caaaaactct tgtagcatat tgttcaattg tctcgtcttt
      541 ggttgtggga acaattaata cagcaatttg cacttgggta ttattgaaaa tagattctaa
      601 cttgtgcgtc agttctttct tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg
      661 ttgctgtaat tgtgggacaa tagttggttg ggctgcaaca aaggaacaca cgaaagtgaa
      721 cattagtatg aaatagcgca ttataaattt ctcattaaat ctggataaat aaattgacgg
      781 cggcatcggt aattttaga
//
LOCUS       DAZPLI010000410          789 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZPLI010000410 DAZPLI010000000
VERSION     DAZPLI010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.413"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..458)
                     /locus_tag="ACHUU3_004591"
     CDS             complement(<1..458)
                     /locus_tag="ACHUU3_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR2084440.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIA"
     gene            complement(530..742)
                     /locus_tag="ACHUU3_004592"
     CDS             complement(530..742)
                     /locus_tag="ACHUU3_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084441.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          203 a          193 c          173 g          220 t
ORIGIN      
        1 gcgatggatt ttgcccgacg cccgcggcta ccgtccggat aaaaatgttc ggtgtaaaat
       61 ccagcctgaa tggttaccca ctcgcgaaaa gcctcaaacg actttagcaa tgccgtatcc
      121 cgggttctgc gtgtcgcaac tgtattcaga tattgctctg cggcttcgct cagagctggc
      181 gtatgttccc gacctactga ttcgcacagg taatcaacga aaccagacag cagttctcgt
      241 tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat cgaatcccag ttgcaggagt
      301 ttgaaaaaac gcttgtggaa tgcgtagtta cgcacacgct taaagtctgc gtgtatccac
      361 tcacctgttt tgatttgatg cagaaaatcg caactctccg gcgtcgccgg gagaagtaat
      421 ccggaagagg tttgtttgat cagttgtata tgcgccatcg tagttctccg ctggcgcagt
      481 agaatgggtg ttcagcccgt tatgtagtat accagaatta atgccaatac taacaggatg
      541 ctctgactcg caattcatcc agcagtttat catttcccat aatgtcactt accctcatcg
      601 gtaaaaaaat tgcctttcga ccattacgat acatcattga ttttttggtt tcagggaagt
      661 aatccatttc gactataact gacaggtcat cacgacgtat gactgcgtat ttactactaa
      721 atagtttctt tattttttcc acgatgcctc caggtttata agtacaaacg gttatatcaa
      781 catagagac
//
LOCUS       DAZPLI010000411          781 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZPLI010000411 DAZPLI010000000
VERSION     DAZPLI010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.414"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>781
                     /locus_tag="ACHUU3_004593"
     CDS             <1..>781
                     /locus_tag="ACHUU3_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR2084442.1"
                     /translation="NETAKQFNTSIIVYLIDPKYFADLPTSQFWSYATYFRVLSFEYL
                     SESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNVSTQAACAKRLNIPEMNGR
                     YFNAGVIYVNLKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKD
                     FDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQS
                     PWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLK"
BASE COUNT          252 a          149 c          148 g          232 t
ORIGIN      
        1 aatgaaacag caaaacaatt taatacctca attattgtat atttaattga cccaaaatac
       61 tttgctgatc tgccgacgtc acagttttgg tcgtacgcga catacttcag ggtattgtcc
      121 tttgaatatc tgagtgaaag tatttccaca ctgctgtatc tggatgccga tgtcgtttgt
      181 aaaggaagtc tgaagcctct cacagaaatt atatttaaag atgagtttgc tgcggtcatt
      241 cctgacaatg ttagtactca ggcggcatgt gcaaaacgcc tcaacattcc cgagatgaat
      301 ggacgttatt tcaatgcagg cgttatctat gtcaatctta aaaaatggca tgaagcaaat
      361 ttgacaccgt atttactcac gcttttacga ggggaaacta aatatggctc tcttaaatat
      421 ttagatcagg atgcgttgaa tatcgcattt aatatgaata atatctacct cgcgaaggat
      481 tttgatacta tttataccct gaaaaacgaa cttcatgatc gtagtcatcg aaagtatcag
      541 caaaccatta ccgataaaac agtattgatt cactatacag ggataactaa accatggcat
      601 agctgggctg gttatccgtc tgcatcatac tttaatatcg cgcgtgaaca atctccctgg
      661 aagaaatatc ctcttaaaga ggcgcggact gttgcagaaa tgcagaaaca atataagcat
      721 ctgtttgccc atggtgagta tattaaaggc ataacttcat taattaagta caagcttaag
      781 a
//
LOCUS       DAZPLI010000412          777 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZPLI010000412 DAZPLI010000000
VERSION     DAZPLI010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.415"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            48..764
                     /locus_tag="ACHUU3_004594"
     CDS             48..764
                     /locus_tag="ACHUU3_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR2084443.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          257 a          135 c          167 g          218 t
ORIGIN      
        1 taacgattta ccagtaatgt aaataaattt tcgaggagat cattccagtg ggacgtaaat
       61 gggccaatat tgttgctaaa aaaacggcta aagacggtgc aacgtctaaa atttatgcaa
      121 aattcggtgt agaaatctat gctgctgcta aacaaggtga acccgatcca gaattaaaca
      181 catctttaaa attcgttatt gaacgtgcaa agcaggcaca agttccaaag cacgttattg
      241 ataaagcaat tgataaagcc aaaggcggcg gagatgaaac gttcgtgcag ggacgttatg
      301 aaggctttgg tcctaatggc tcaatgatta tcgccgagac attgacttca aatgttaacc
      361 gtacgattgc taacgttcgc acaattttca ataaaaaagg cggcaatatc ggagcggcag
      421 gttctgtcag ctatatgttt gacaatgctg gtgtgattgt atttaaagga tcagaccctg
      481 accatatttt tgaaatttta cttgaagctg aagttgatgt tcgtgatgtg actgaagaag
      541 aaggtaacat tgttatttat accgaaccta ctgaccttca taaaggaatc gcggctctaa
      601 aagcagctgg aatcactgag ttctcaacaa cagaattaga aatgattgct caatctgaag
      661 ttgagctttc cccagaagat ttagaaatct ttgaagggct tgttgatgcc cttgaagatg
      721 acgacgatgt acaaaaagtt tatcataacg tcgcaaatct ctaattatct tttaaag
//
LOCUS       DAZPLI010000413          775 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZPLI010000413 DAZPLI010000000
VERSION     DAZPLI010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.416"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..344
                     /locus_tag="ACHUU3_004595"
     CDS             <1..344
                     /locus_tag="ACHUU3_004595"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR2084444.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKAAAAELALG"
     gene            complement(406..>775)
                     /locus_tag="ACHUU3_004596"
     CDS             complement(406..>775)
                     /locus_tag="ACHUU3_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HFR2084445.1"
                     /translation="QDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGW
                     QQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGDC
                     QGYLFRCLHCGKLRLWGDFS"
BASE COUNT          209 a          190 c          176 g          200 t
ORIGIN      
        1 gtgcagatgg tcatttttgt ggcaatttgc cgaatacgac ccatttccat gagcaaaccg
       61 tggagttccc gatccaggga gagatggttg atattgtggc gcatggtgag ttgggcggcg
      121 acttttcgtt agtgcctgac agctacgtga cgatggggcc gaaaagcatc atggcggcga
      181 aaaacttgct gataattgtc agtggcgcag gtaaagcgca ggcactgaaa aatgtgctgc
      241 aaggtccggt taccgaagat gttccagctt ccgtgttgca gctgcatcct tcgctgatag
      301 taatcgctga taaagccgct gccgcagaat tagcgctcgg ttaagctgaa gaaataaggc
      361 cggatgtatg aattacgctc cggcctgttc tcattattta aataactacg aaaaatcacc
      421 ccacagtctc agcttgccgc agtggagaca gcggaagaga taaccctgac aatcaccacc
      481 cttttgcagg catttagcta gatcagaatt tctaataccg aaattctcac aatcttcttc
      541 aaggttggca aattcatcga ggcgatcttt tatatcattc cagcccacat agccgataaa
      601 agcacagaaa tcgccacaat gcgcaagcca gaattcctgt tgccatccat gataacctgg
      661 cgtgcgttca accaactctt tcagcatttc atcaggatat gtgttcttaa taccggcaaa
      721 ttcgtcctct tcatcatact caaattcaac accttctatg ctggcatcat cctga
//
LOCUS       DAZPLI010000414          769 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZPLI010000414 DAZPLI010000000
VERSION     DAZPLI010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.417"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            175..>769
                     /gene="ais"
                     /locus_tag="ACHUU3_004597"
     CDS             175..>769
                     /gene="ais"
                     /locus_tag="ACHUU3_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR2084446.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFV"
BASE COUNT          209 a          153 c          165 g          242 t
ORIGIN      
        1 aatattgaaa tttttatatt ttttcttatt taggctttgc atttggcaaa attttgaggc
       61 attttgccga catcgtagga tttttaatat tacaccaact gctaattatc gccagaaatg
      121 tcgctcaatt tcacggtaat tgtctggttg cgcttgtcta taggtggagt ttacgtgtta
      181 gctttttgcc gctcatcatt gaagtcaaaa aaatatttca tcattttact ggcgctcgct
      241 gcaattgccg gactgggtac tcatgccgcc tggagtagca atggtttgcc acgtatcgac
      301 aataaaacat tggccagact ggcacagcag cacccggttg tcgttttgtt tcgccatgct
      361 gaacgttgcg atcgttcaac caatcaatgc ttgtcagata aaacaggtat tacggttaaa
      421 ggtacccagg atgcccgtga actgggcaac gcttttagcg ctgacatccc tgatttcgat
      481 ctttattcca gtaataccgt ccggaccatt cagtcggcga cctggttttc cgcgggtaaa
      541 aaactgacgg tagataaacg acttcttcag tgcggtaatg agatttatag tgcaattaag
      601 gacttacaaa gcaaagcgcc tgataaaaat atcgttattt tcacccataa tcattgcctg
      661 acatatattg ctaaaaataa gcgtgatgcg acatttaaac ctgattatct ggatggttta
      721 gtcatgcatg tggaaaaagg caaagtttat ctggatgggg aattcgtta
//
LOCUS       DAZPLI010000415          765 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZPLI010000415 DAZPLI010000000
VERSION     DAZPLI010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.418"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(81..>765)
                     /locus_tag="ACHUU3_004598"
     CDS             complement(81..>765)
                     /locus_tag="ACHUU3_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR2084447.1"
                     /translation="QMQSQMDPVYTPARKIHLYHCDYRGLPLALISTEGTTAWCAEYD
                     EWGNLLSDENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPVQYIDSMGLASKYGHLNNGGYGAKPSKPPTPDPSKLPDIAKQLRLPYPI
                     DQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCMPQDDPQYPGMETKTASDYLPQ
                     TNWSTTQLR"
BASE COUNT          177 a          193 c          188 g          207 t
ORIGIN      
        1 ggagggacag ctgatagaaa cagaagccac tggagcacct caaaaacacc atcatacact
       61 aaatcagtaa gttggcagca tcaccgtaat tgagttgttg accaatttgt ctgtggcagg
      121 taatcacttg ctgtctttgt ctccatccct ggatactggg gatcatcctg cggcatacaa
      181 gtcagattgg gttttaccca cttcctgcaa tacagtgtgt aatcgtaagg gcttaatgct
      241 ctgaagaatg ttttgaaaac attaggcgca ctactggcct ggtcaatagg atatggcagt
      301 cttaattgtt tcgctatgtc tggcaactta cttggatcgg gcgtaggcgg tttgctgggt
      361 ttcgctccat atccgccatt atttaagtgt ccatatttag atgccagccc cattgaatct
      421 atatactgaa ccggattcaa cggatactga taaaaattcc atcccccctt cagtccaatc
      481 ggatcctgag tgatataccg cccctgcagc gggtcataat agcggtggcg gttgtaatac
      541 aggccggact cctcatcata ctgctgcccc ggcaggcgaa tgagctgctg cagatgatgc
      601 gggttctcat cactcagcag gttgccccat tcatcatatt ctgcgcacca cgctgttgtc
      661 ccttccgtgc tgataagggc cagcggcagg ccgcgatagt cgcagtggta caggtgaatt
      721 tttcgcgccg gcgtgtatac cgggtccatc tggctttgca tctgc
//
LOCUS       DAZPLI010000416          761 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZPLI010000416 DAZPLI010000000
VERSION     DAZPLI010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.419"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..516
                     /locus_tag="ACHUU3_004599"
     CDS             <1..516
                     /locus_tag="ACHUU3_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HFR2084448.1"
                     /translation="LESALKEAVTEWRLYPVVEALQAMRGIQFITAVGLISELGDLTR
                     FEHPRQLMSWFGITPSEYSSGGSRHQGSITKAGNSYARKLLVEAAWSYRHPARISPAI
                     QKRQENLPRPVIDRAWDAQLRLCKRYRKLQAKGKNVNITIVAVARELAGFIWDMGRIA
                     MSVAQQPQCHK"
BASE COUNT          222 a          174 c          198 g          167 t
ORIGIN      
        1 ctggaatccg ccctgaagga ggccgttacc gaatggaggc tgtatccggt agtagaggcg
       61 ctgcaagcca tgcgagggat tcagtttatt actgcggttg gattaatttc tgaactgggt
      121 gatctgaccc gttttgaaca tccacgacaa ctgatgtcat ggtttggtat cacgccatca
      181 gaatattcat cgggtggaag cagacatcag ggcagcatta ccaaagccgg aaacagctat
      241 gccagaaagc tattggtcga ggcagcgtgg agttaccgtc atccggcacg catcagtccg
      301 gcaatacaaa aaaggcagga aaatttaccc cgccccgtca ttgacagagc atgggatgct
      361 caactcaggc tttgtaagag gtatcgaaaa cttcaggcca aaggaaagaa tgtcaatatt
      421 acaattgttg ctgttgcacg tgagctggcg ggttttatct gggatatggg ccgaatagca
      481 atgtctgtcg cacaacaacc acaatgtcac aaataaaaaa tgagaataac cagttccctg
      541 aaggcgacgt agcccggtac aagagtaacc cacgttagcg cttggcgacg gcggaacaaa
      601 gctgattcgc gacgtaagaa agtggcaggc tcatatgacg gacctctgta atgcggtatc
      661 caacccgcga atatcagcgt gattcaccgt cggatttact gctacgtcgc cctcagggaa
      721 ttaaacctaa aaagaaaatc gtcaaaatta gagattccgg t
//
LOCUS       DAZPLI010000417          751 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZPLI010000417 DAZPLI010000000
VERSION     DAZPLI010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.420"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            584..>751
                     /locus_tag="ACHUU3_004600"
     CDS             584..>751
                     /locus_tag="ACHUU3_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR2084449.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVID"
BASE COUNT          213 a          135 c          164 g          239 t
ORIGIN      
        1 cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact tcatatttga
       61 acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat cgtaaaggaa
      121 accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt cgatagcgcg
      181 agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat tattcaatac
      241 aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt ctctaaaaaa
      301 ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg tcagggatta
      361 aaagatgaac gtaaatttat ttaacgcatt aattttaaag ggttttattg tttgttgacg
      421 aaaacaggaa tcgtgttcgg tctcttttta tctgttaaaa gccagaagca tttccttcgc
      481 tgactttata gtcaaccata acacacactc tactgtctga gtccaacgtt ttttaacatt
      541 cttgttaaaa ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac agcctgcgcc
      601 cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg gcgatataca
      661 cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc cgtggcgaga
      721 tttacttatc tcagtgggag acgttatcga t
//
LOCUS       DAZPLI010000418          748 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZPLI010000418 DAZPLI010000000
VERSION     DAZPLI010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.421"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..407
                     /locus_tag="ACHUU3_004601"
     CDS             <1..407
                     /locus_tag="ACHUU3_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR2084450.1"
                     /translation="WRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYT
                     QRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELAILTWVDWY
                     NNRRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            449..649
                     /locus_tag="ACHUU3_004602"
                     /pseudo
     CDS             449..649
                     /locus_tag="ACHUU3_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          216 a          175 c          198 g          159 t
ORIGIN      
        1 ggtggcgggt ctcatcgtcc atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tatgggcccg tcgaccgtcc ggcacggtcc atcacagtga taaaggttct cagtatgtat
      121 cgctggccta cacacagcgg cttaaggaag ccggattact ggcatcaaca ggaagtacag
      181 gcgactcgta tgacaacgcg atggcggaga gcatcaatgg tctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccgtgcag aagtggaact ggccatactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tactcctccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaacg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcaa ccttcacgat aaaaaacttt tcattaaagg
      481 taaagcggat caatatccgg cacttcattc tctgatatct ccgacccgca ttaatctgaa
      541 agaaatagaa attcactggc gtgaagtgct acgtctggcg acctcaataa aacagggaac
      601 agtgacagca tccctgatgc tgagaaaact tgccagttac cccaaacaaa agtaaggcag
      661 tatgactttc cgtgagacag atgctggcca acgtgggata tttaacgcgg gttaacatcg
      721 ttgtcagagc cgcatgggtg agctgttg
//
LOCUS       DAZPLI010000419          746 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZPLI010000419 DAZPLI010000000
VERSION     DAZPLI010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.422"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            1..746
                     /gene="espX1"
                     /locus_tag="ACHUU3_004603"
                     /pseudo
     CDS             1..746
                     /gene="espX1"
                     /locus_tag="ACHUU3_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
BASE COUNT          280 a          126 c          107 g          233 t
ORIGIN      
        1 ttagtaaaag tattttgcga gtataaaaca gagttcttaa ttttgtttga tgactttttt
       61 agaagccata acttgcctaa accatctcca gtccttcatc atttttttca atatactcat
      121 ctgagagatg catattttta tagatgtaaa ctcatagaac atacagttca attttatttt
      181 ttaaacacaa aggtatcaca ttacttagat tggatgtttt tgatgacaga acaagcgaat
      241 gtctttctga agagattaag atataccaag aatgccatga aaaattcgtt aattttttaa
      301 aggcaaactt caaccaagaa atatacccac agctatatac tcctgaaata ttttatgagg
      361 cctgcagaaa tctccagtca ttttatgatc atcaggaaac gtcacaaaac gcaaaataca
      421 gtgcaataga caagaaaaaa agtcatctta accaggaaat caaaaagctt attaaaaaaa
      481 atattttccc tgagctatac aatgagcaat gcaataaaat cccctcctca tcgatagatg
      541 ataaccaggg aataatgtgg cagaacttta aaactagcaa ttcggcatat tcaaaactat
      601 gtggaaaatt atctttattt aaaagcgtgc ccagtcggtt aatagaaaaa tcagcttact
      661 gctcaactga aaacatggcc actaataaat ttaatgtcgt cttttcttac tgcggagata
      721 atataaagga atttatactt ttatta
//
LOCUS       DAZPLI010000420          738 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZPLI010000420 DAZPLI010000000
VERSION     DAZPLI010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.423"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            82..228
                     /locus_tag="ACHUU3_004604"
                     /pseudo
     CDS             82..228
                     /locus_tag="ACHUU3_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
BASE COUNT          227 a          124 c          140 g          247 t
ORIGIN      
        1 ctctgaagag aacctcgtta atgattggtc ttgctcattg ttaataacaa aatgttgaat
       61 ttaccgatta ggaaactttt tcctacgcgg attgcctcca gctatggcat gaactttgag
      121 tttatgccag aactgaagtg gagctttggc taccctggcg cgattatctt tatgatcctt
      181 gcgggcctga caccgtatct gtactttaag cgtaagaact ggctgtaact atacgatgtc
      241 gctgtgagta agttagtgtg ctgacagcga cattttagcc gagggaatcg cagataacat
      301 aattgttgtg aataatatcc atgccacgtt gagttggacg cttaatgact cacatctcac
      361 cccttcaatt gctgcagaga taatagaaaa aagtgtcaaa cccaatgaaa tcaatacaaa
      421 gtagattgcc ttacatccag tatctcttat tctttttgct ccaatattga tatttacata
      481 ggtattatta ataaccagaa taaaactcaa agccaggttg atatcattca aagttattaa
      541 tattgaccat gatgttaatg tatagattat gaactgaaag cgtgataaac gctcaagcct
      601 ggatgggtag aatatatttc tgatggatga tttgaacatt tttacattgt tttatatata
      661 taattgtttt gaatatatat tacctgaatt atgtggtttg catatatata tgcaatatag
      721 cagtctttgc attgataa
//
LOCUS       DAZPLI010000421          727 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZPLI010000421 DAZPLI010000000
VERSION     DAZPLI010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.424"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            165..727
                     /locus_tag="ACHUU3_004605"
                     /pseudo
     CDS             165..727
                     /locus_tag="ACHUU3_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          245 a          141 c          127 g          214 t
ORIGIN      
        1 taaaaaacaa tctgaagcat gctctgaaac atcctactct gtcactctaa tgatggcacc
       61 ttgctcttct tataaagaag ggaacaatta agaatattaa attttcaaac tttcatcaac
      121 ttcacatcaa gaaattaaaa aatttaaaca ttaatctaaa taataacaac ctgatcacat
      181 aatcgggtac gattttatcc gataactata atgctataac tctgggaaat ggttggaata
      241 caccgctggg tgccatctct ttttacgctg cccgatcgag tagtaagtta aataatgata
      301 ccagacacga aggtacgagc tatcaggtcg cctataataa atatctagta caaacagcca
      361 ctcgatttag cgtggccgcc tggcgttatg cttcgcagga ttacaggaca ttcagcgacc
      421 atctttacga aaatgataaa cactatcatc agagtgatta tgatgatttt tatgatattg
      481 gcagaaaaaa tagcctttct gccaatatta tgcaaccttt atccaataat ctgggaaatg
      541 tatcattaag tgcgctttgg cggaattact gggggcgaag tggaaatgct aaagattacc
      601 aattcagtta ctccaataac tggcaacaca ttagttatac tttctctgcc agccaatctt
      661 atgatgaaaa taataaagaa gaggagcgtt ttaatctgtt tatctccatt cctttctact
      721 gggggga
//
LOCUS       DAZPLI010000422          726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZPLI010000422 DAZPLI010000000
VERSION     DAZPLI010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.425"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            35..>726
                     /locus_tag="ACHUU3_004606"
     CDS             join(35..284,284..>726)
                     /locus_tag="ACHUU3_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HFR2084451.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHY"
BASE COUNT          167 a          183 c          205 g          171 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 tgcacgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAZPLI010000423          715 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZPLI010000423 DAZPLI010000000
VERSION     DAZPLI010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.426"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>715
                     /locus_tag="ACHUU3_004607"
     rRNA            <1..>715
                     /locus_tag="ACHUU3_004607"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          203 a          155 c          220 g          137 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact
      481 gcgaataccg gagaatgtta tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa
      541 gagggaaaca acccagaccg ccagctaagg tcccaaagtc atggttaagt gggaaacgat
      601 gtgggaaggc ccagacagcc aggatgttgg cttagaagca gccatcattt aaagaaagcg
      661 taatagctca ctggtcgagt cggcctgcgc ggaagatgta acggggctaa accat
//
LOCUS       DAZPLI010000424          705 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZPLI010000424 DAZPLI010000000
VERSION     DAZPLI010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.427"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            2..>705
                     /gene="istB"
                     /locus_tag="ACHUU3_004608"
     CDS             2..>705
                     /gene="istB"
                     /locus_tag="ACHUU3_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HFR2084452.1"
                     /translation="MSDNLLNKLTQLKLPAMAGSLIRQRETPQTYDELSFEERLTLLV
                     DDELLSRENSRVARLRKNACLKYQATPEGLRYPASRGLRAEQMRELLNGHYIIHRKNL
                     LITGPTGCGKSWIANALGEQACRQKYSVRYCRTGRLLEQLAQGRVDGSWLKYLKQLQK
                     IQVLILDDLGLEQLSNAQCNDLLEITEDRYGQSSTIVVSQFPVDKWHGLMENPTTADA
                     ILDRLVHNSHRVVLQG"
BASE COUNT          211 a          158 c          202 g          134 t
ORIGIN      
        1 tatgagtgat aatctgttaa acaaactgac ccaactgaaa ctcccggcga tggccgggag
       61 cctgatacgg caacgggaaa cgcctcagac ttacgacgaa ctgtcgttcg aggaaaggct
      121 gacactgctg gttgatgacg agcttctgag cagagaaaac agtcgtgtgg cacggttgag
      181 aaaaaatgcc tgcctgaaat atcaggcaac accggaaggg ctgcgttatc cggcctcaag
      241 aggtctgcgg gctgaacaga tgagggaact gctgaacggc cactacataa tccacaggaa
      301 aaacctactg ataaccggtc cgacaggatg cggtaagagc tggatagcca atgcgctggg
      361 cgaacaggca tgtcgacaga aatacagcgt ccggtactgt agaaccgggc gattactgga
      421 acagttggct cagggacgtg ttgatggcag ttggctgaaa tatctgaaac agttgcaaaa
      481 aatacaggtt ctaatcctgg acgatctggg gctggaacag ttaagtaacg cccagtgtaa
      541 tgaccttctg gagatcacag aggatcgtta cggacaaagc agcacgatag tggtaagcca
      601 gttcccggta gataaatggc atggtctgat ggagaaccca acaacagcag atgccatcct
      661 ggacagactg gtacataact cacacagagt ggtccttcag ggaga
//
LOCUS       DAZPLI010000425          705 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZPLI010000425 DAZPLI010000000
VERSION     DAZPLI010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.428"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            282..686
                     /gene="stpA"
                     /locus_tag="ACHUU3_004609"
     CDS             282..686
                     /gene="stpA"
                     /locus_tag="ACHUU3_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR2084453.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT          213 a          139 c          158 g          195 t
ORIGIN      
        1 tcttttattc tgttatattg catttttatt cacttcggtt ttgtcaggct tgcggaatta
       61 gcgagcagag agcgccgttc tgttcaccaa taatctaaat tactcatcaa aatggctaat
      121 atataatctt gaaattattc tgagagtttc agaaaataac agctgaaata atctcgcgca
      181 agactgtaaa tagattaaat tttgtggaaa tataataagt gatcgcttac actacgcgac
      241 gaaatacttt tttgttttgg cgttaaaagg ttttctttat tatgtccgta atgttacaaa
      301 gtttaaataa cattcgcacc ctccgtgcga tggctcgcga attctccatt gacgttcttg
      361 aagaaatgct cgaaaaattc agggttgtca ctaaagaaag acgtgaagaa gaagaacagc
      421 agcagcgtga actggctgag cgtcaggaaa aaattagcac ctggctggag ctgatgaagg
      481 ctgacggaat taacccggaa gagttattgg gtaatagctc tgctgctgca ccacgcgctg
      541 gtaaaaaacg ccagccgcgt ccggcgaaat ataaattcac cgatgttaac ggtgaaacta
      601 aaacctggac cggtcagggc cgtacaccga agccaattgc tcaggcgctg gcagaaggta
      661 aatctctcga cgatttcctg atctaattac tgaggccgga tgtcg
//
LOCUS       DAZPLI010000426          698 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZPLI010000426 DAZPLI010000000
VERSION     DAZPLI010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.429"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>698
                     /locus_tag="ACHUU3_004610"
     CDS             <1..>698
                     /locus_tag="ACHUU3_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084454.1"
                     /translation="IAQQKAKTEKQNQRAQAAAARAQARQQQQIAARKEKAELKLLDV
                     SSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKINGNRK
                     AQFAPVKGGSGNSATMGALLFGGATAAAGVMAVSKTSEFMANSFANAETEGELIQRAR
                     LGGVDYNLLNNLERWAYDNGIDSLQGDPGRRKILDAIGKDVKERATKSYDEAEYVVDK
                     KTGKGIWKGGDSGI"
BASE COUNT          238 a          138 c          182 g          140 t
ORIGIN      
        1 attgctcaac agaaagctaa aacagaaaag cagaatcaaa gagcacaagc tgccgcagca
       61 agagcacagg caaggcaaca gcaacaaata gcagcacgta aagagaaagc agaattaaaa
      121 ctccttgatg tatcaagctc tatcagtgca atgcaccgtc tctctgtagc ggaacaatac
      181 aaagctattg cacaggctag agagatagca aaacaatatg agcaaggggc tatcagcctg
      241 gcgcgtatga atagtcagat gaaacgccta caacagcaac aacgtaagat taatggcaac
      301 agaaaagctc aatttgcccc cgttaaggga ggatcaggga attccgccac gatgggcgct
      361 ctgttatttg gaggcgctac ggctgctgct ggcgtgatgg ctgttagtaa aacctctgaa
      421 tttatggcta acagctttgc taatgctgaa acagaagggg agctaatcca acgtgcaagg
      481 cttggtggag ttgattacaa cttacttaat aatcttgagc gttgggcgta tgacaacggg
      541 atcgatagcc tccagggtga tccgggacgc agaaaaatac tagatgccat cggtaaagat
      601 gtaaaggaac gcgctaccaa aagctatgac gaggctgaat atgtagttga taagaaaaca
      661 gggaaaggca tatggaaggg cggtgattct ggtattaa
//
LOCUS       DAZPLI010000427          695 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZPLI010000427 DAZPLI010000000
VERSION     DAZPLI010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.430"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>695
                     /locus_tag="ACHUU3_004611"
     CDS             <1..>695
                     /locus_tag="ACHUU3_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR2084455.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVY"
BASE COUNT          162 a          167 c          205 g          161 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacat
//
LOCUS       DAZPLI010000428          690 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZPLI010000428 DAZPLI010000000
VERSION     DAZPLI010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.431"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
BASE COUNT          241 a          105 c          143 g          201 t
ORIGIN      
        1 agtctatttt ataaaaaaat taagtaggct aagaagtacc ttagaaatca attttaaggg
       61 gtctaaaacg ctctgtaacg agctaaactg tataagtggc ataagatgcc ttttagactg
      121 acactcgctt agagagcttc ctagagcttc tgggaggatt cattagatat cgttgtatac
      181 agagacacag tatctataaa gggtattaag agattcttag aagttactgt aaagaaccac
      241 taagataaga agagagggac aaagaaccga aggaaagtcc cttattaagt aggagtctta
      301 atagactgtc ttaatagata acttaaaagt cttaaaggat atcttccaag aagagaggaa
      361 cgaagaacca gaggaagtga ccgttgaccg tttgaacaga ctgtttaagt aaagcctata
      421 aagtatctat taattaaggg ctatgtctca atactagcag tttgtactag cagtttgtca
      481 atacctttgt ctttagcaat tcgtgctatt tattaaatat agctgttaaa acagagtatg
      541 ctttttaacc taaaaccttg tcgcttcgct ctgggtcaca tgttcttggg ttcttagtga
      601 gagttacact atcttctaag gtactcaata aaaagacgag taacaaccga agaacgagga
      661 tagaggtatt gtaataactt agaaggtgtc
//
LOCUS       DAZPLI010000429          684 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZPLI010000429 DAZPLI010000000
VERSION     DAZPLI010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.432"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..560
                     /locus_tag="ACHUU3_004612"
     CDS             <1..560
                     /locus_tag="ACHUU3_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR2084456.1"
                     /translation="RFSQLESALNTQKNSIPALEKEVKALDKQMVAAQKAADAYWGKD
                     ANGKQMTREEAFKKIHQQRDEFNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVP
                     IQQKRDFDINEQRRQTFLQSQKLSRKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMK
                     IDDINQANERWKKDTEQLRRNGVIK"
     gene            complement(616..>684)
                     /locus_tag="ACHUU3_004613"
     CDS             complement(616..>684)
                     /locus_tag="ACHUU3_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084457.1"
                     /translation="ALMMITYIPQISLFIPQLLGLM"
BASE COUNT          244 a          122 c          165 g          153 t
ORIGIN      
        1 atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt cctgcgctgg
       61 aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca gcggatgcat
      121 attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt aaaaaaattc
      181 accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg gttaaatatg
      241 acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa gagtgttatg
      301 aagtgccgat tcagcagaaa cgagatttcg atatcaacga acaacggcga cagacttttc
      361 tgcaatcaca aaaactcagc cgtaaattgc aggatgactg ggtaacgctc gaaaaagggc
      421 aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg attttgatga
      481 aaattgacga tatcaaccaa gctaatgaac gctggaaaaa agataccgaa caactgcggc
      541 gcaatggggt gattaagtaa ctcatgttaa gtgcctttgc cggataatat aaaaagttat
      601 ccggcaaatt attttttaca ttagccctag tagctgtgga atgaacaagg atatttgcgg
      661 aatgtaagtg atcatcatca acgc
//
LOCUS       DAZPLI010000430          664 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZPLI010000430 DAZPLI010000000
VERSION     DAZPLI010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.433"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            52..664
                     /locus_tag="ACHUU3_004614"
                     /pseudo
     CDS             52..664
                     /locus_tag="ACHUU3_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          205 a          161 c          171 g          127 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgt
//
LOCUS       DAZPLI010000431          659 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZPLI010000431 DAZPLI010000000
VERSION     DAZPLI010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.434"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..494)
                     /locus_tag="ACHUU3_004615"
     CDS             complement(<1..494)
                     /locus_tag="ACHUU3_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084458.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDGK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDA"
BASE COUNT          210 a          145 c          122 g          182 t
ORIGIN      
        1 ttcgcatcac ccggacagtt agaagaggct acccaccaag catttttata taccgcacca
       61 ttaaagacga cataccaggt ctgaccgccc tgctgtttat tccaggcaac tacagaagaa
      121 ttagacggaa cagtgccctg tgctggtgta gccgaaccgc cattagccgg cgtattggat
      181 gcaggcgtat ttgaatttga agaagatgag ctgccgctct tttcgcagga aagtgtattg
      241 ccaaactgac taatttcagc agctgttgcg gtacgcttta aacgccacgg gttagttgca
      301 tcatttgctt tcgcttttcc cgggcaattt gtagaaaaac cccaccaggc attttcataa
      361 attttgccat cgaaaataac ctgatattta ttaccacctt gttggttatt ccatgcttcc
      421 attgccaatg ctggcagagc ggaacacacc agccccatac caatcataga tttagtaaat
      481 atatttaatt tcattataat cccttgtgac gtaaaaactg caaaacaaaa gtcctacgca
      541 ggaattattt acgttttacg agaacccttc aatatgaatt aaagtgaggc attaaaaata
      601 agaaaaaagc ctgacaaatg aagaatttta aaaacagaaa cattcatatt taaaatatt
//
LOCUS       DAZPLI010000432          650 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZPLI010000432 DAZPLI010000000
VERSION     DAZPLI010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.435"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..194
                     /locus_tag="ACHUU3_004616"
     CDS             <1..194
                     /locus_tag="ACHUU3_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR2084459.1"
                     /translation="VDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNL
                     HYIDTGAVFGGELTLVQLQ"
     gene            complement(195..386)
                     /locus_tag="ACHUU3_004617"
     CDS             complement(195..386)
                     /locus_tag="ACHUU3_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR2084460.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(491..>650)
                     /locus_tag="ACHUU3_004618"
     CDS             complement(491..>650)
                     /locus_tag="ACHUU3_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR2084461.1"
                     /translation="LTIPCKSDPDLCMQLDAWDAETSIPALLNGEHSVLYRTRYDQQS
                     DAWIMRLA"
BASE COUNT          184 a          148 c          160 g          158 t
ORIGIN      
        1 acgttgattt gcatcaggtc ttgtggagcc gctcgcgatt aggtgaacgc caaaaagggc
       61 agggaattac aggtgctgat catttctggt ttggtcatac accgttgcga catcgcgtgg
      121 atattggcaa cctgcattat attgataccg gtgctgtctt tgggggcgaa ctgactcttg
      181 tgcaattgca ataattaaaa atcaccgtac tcctgtgcag gtcgccagaa accatctata
      241 aaatcctcaa tcggaaaaca accgccatgg cggatccgtt gatcgctcat agaataaaga
      301 cactgctgtt ccgtgttgta gacatccaca acaatatctt cacaaccgcc atccaggtag
      361 caaacaaaaa gtaccagcgc gaacatttca tccccgaagt gtggtgccgt accgttaagt
      421 ttaggagaga ttttacaacg ggggaataac caggacaaat aacccgccat cccgacgggt
      481 tctttttgga tcaggcaaga cgcataatcc aggcatcaga ttgttgatcg taacgggtac
      541 ggtaaaggac agagtgttcg ccatttagca gggcagggat actggtttca gcatcccagg
      601 catcgagttg catacataaa tcggggtcag atttacaagg aatggttaac
//
LOCUS       DAZPLI010000433          649 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZPLI010000433 DAZPLI010000000
VERSION     DAZPLI010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.436"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(26..330)
                     /locus_tag="ACHUU3_004619"
                     /pseudo
     CDS             complement(26..330)
                     /locus_tag="ACHUU3_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            384..587
                     /locus_tag="ACHUU3_004620"
                     /pseudo
     CDS             384..587
                     /locus_tag="ACHUU3_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          158 a          175 c          153 g          163 t
ORIGIN      
        1 gcatcatttt ttccagaggc ggtcaaactc cgccttcgca aaataagcgg aagcctggcg
       61 aaggatatcg ttactgcggc gcagttcacg attttcacgc tccagctctt tcagacgctg
      121 acgttcagcg gtggtgagcc caccatcacc gcccccggta tcccgctcat gctggcgaac
      181 ccagacacgc agagtctccg gcgtacagcc aatctttggg gcaatggaac aaattgccgc
      241 ccactgtgag tcatattcat cctgactttc cagaaccata cgaatcgccc gctgacggac
      301 ttcgggggaa aaacgagtat ttttagtcat cctgtttacc tctttctcag ggagtttagt
      361 ctccaggatt tccggggcgg ttcagacatc tagaatttac tgtcagcacc ggcgttaaag
      421 tttacttctg cgatcctcag agtccttggc agcggggaac aaatgagaat acaaatgggc
      481 taattcggca gtactttcct aaaaagacat gtcttgccca atatactcaa catcgagtag
      541 gcagcctgac ggctgcggct tgtcatggtc tgcaattacc gttataaaaa caggcaatgt
      601 cattgtcttt caggtggtta tatggcccgt tcagctaaac cccgtaaac
//
LOCUS       DAZPLI010000434          644 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZPLI010000434 DAZPLI010000000
VERSION     DAZPLI010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.437"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..>644)
                     /locus_tag="ACHUU3_004621"
     CDS             complement(<1..>644)
                     /locus_tag="ACHUU3_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084462.1"
                     /translation="RNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFTD
                     CSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGDL
                     TAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDP
                     VDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPA"
BASE COUNT          107 a          211 c          179 g          147 t
ORIGIN      
        1 gccggttcac cgttcaccgt gcgccgggtg atacggtccg acgcatcgta atgccagagg
       61 gtgataagcc cctcatcttc actctgcgtg agttttccgg tcaggtcata ctggtaacgt
      121 tgcgtccggc cgtcaaagcc gcgctgctcc gtcagcctgt cgaccgggtc ataccggaac
      181 gtggactggc tgccgttctc gttggtcagc acagtgatgc gcccggcggc gtcatacccc
      241 atgcttcgcg tcagaccgcc ctgcgtggtg ctgacggcct ttccccacgc atcatactgt
      301 atctcactgc ggctgccgtc cggggcaacg atagcggtga ggtctcctgc ggcgctgtac
      361 tcataacggg tttcacgccc ctgcgcatcc ctctgactga ccagttgacc gcgcgggtta
      421 taactgctgt aagtgctgat gccttcttcc cggtgaacgg cgatttgttg accgtaccgg
      481 tcatactcat accgcgtcgt gtaccccgag cagtccgtaa acgcctgaag ctgaccgtac
      541 cggtttcgcg ttattctccg cctcaccccg gttccgtctt ccaccgcaca gggcaggcca
      601 ctgcgggaag aatcataaaa ccagcgcgta atgttaccgt tgcg
//
LOCUS       DAZPLI010000435          641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZPLI010000435 DAZPLI010000000
VERSION     DAZPLI010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.438"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>641
                     /gene="fliD"
                     /locus_tag="ACHUU3_004622"
     CDS             <1..>641
                     /gene="fliD"
                     /locus_tag="ACHUU3_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR2084463.1"
                     /translation="VSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDES
                     SDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLVL
                     TASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSSN
                     KITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          217 a          146 c          139 g          139 t
ORIGIN      
        1 aagtttccac cgaagcaggt gctgcccccg gtatctatac catcagcgta acccaactgg
       61 cgcaggcgca atctttacga actgattccc cgacaatcat tgcatcaaca aaagatgcac
      121 tgggtgatga gtcatcagat acacgcacaa taaaaattac ccaagacggt cgtaaagagc
      181 cgctggaaat caagctcaat aaagatcaga cgtcattgga tgagatcagc aaagccatta
      241 acgatgccga tagtggaatt tccgctagta tcgttaaagt caaagatggt aactatcaat
      301 tggtattgac cgccagtgaa ggtctggcca ataaaatgac catatctgtt gagggtgaca
      361 gtaaattaaa tgatctgctg gcttatgaca gcaagaccaa caccgggaat atgaaggaac
      421 tggttaacgc gcaaaatgca cagctaaacg ttaacggtat cgatattgaa cgttcaagta
      481 acaaaattac cgatgctcct cagggagtga ctctcgattt gaccaaaaaa gtcactgacg
      541 tccgggtcac ggtgacaaaa agcaacgata aagcaaccga agcaattaaa ggctgggtgg
      601 attcatataa ctccctcatt gacaccttta acaccctgac a
//
LOCUS       DAZPLI010000436          637 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZPLI010000436 DAZPLI010000000
VERSION     DAZPLI010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.439"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>637
                     /locus_tag="ACHUU3_004623"
     rRNA            <1..>637
                     /locus_tag="ACHUU3_004623"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          152 a          143 c          209 g          133 t
ORIGIN      
        1 tcctcctaaa gagtaacgga ggagcacgaa ggttggctaa tcctggtcgg acatcaggag
       61 gttagtgcaa tggcataagc cagcttgact gcgagcgtga cggcgcgagc aggtgcgaaa
      121 gcaggtcata gtgatccggt ggttctgaat ggaagggcca tcgctcaacg gataaaaggt
      181 actccgggga taacaggctg ataccgccca agagttcata tcgacggcgg tgtttggcac
      241 ctcgatgtcg gctcatcaca tcctggggct gaagtaggtc ccaagggtat ggctgttcgc
      301 catttaaagt ggtacgcgag ctgggtttag aacgtcgtga gacagttcgg tccctatctg
      361 ccgtgggcgc tggagaactg aggggggctg ctcctagtac gagaggaccg gagtggacgc
      421 atcactggtg ttcgggttgt catgccaatg gcactgcccg gtagctaaat gcggaagaga
      481 taagtgctga aagcatctaa gcacgaaact tgccccgaga tgagttctcc ctgacccttt
      541 aagggtcctg aaggaacgtt gaagacgacg acgttgatag gccgggtgtg taagcgcagc
      601 gatgcgttga gctaaccggt actaatgaac cgtgagg
//
LOCUS       DAZPLI010000437          628 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZPLI010000437 DAZPLI010000000
VERSION     DAZPLI010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.440"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..547
                     /locus_tag="ACHUU3_004624"
     CDS             <1..547
                     /locus_tag="ACHUU3_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084464.1"
                     /translation="QIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQVEKVGIIRALT
                     VGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTG
                     QTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAPVINWNCGATQ
                     PVPDAPVPKDLPPGMPDMRQF"
BASE COUNT          175 a          127 c          191 g          135 t
ORIGIN      
        1 ccagatccag aacattggcg ttaatcagat acagacggtt ggtgtcaacc aggtggaaac
       61 ggtgggcagt aaccagatta tcaaagtggg atcaaaccag gttgaaaagg tggggatcat
      121 tcgtgcgctg acggtgggtg tggcttacca gacgacggta ggcggcatta tgaatacctc
      181 ggtggcgttg ttgcagtcct cacaggtagg gctgcataaa tcactgatgg tgggaatggg
      241 ctacagcgtc aatgtaggga ataacgtcac cttctcggtg ggcaagacga tgaaggaaaa
      301 caccggacaa acagcagttt attctgccgg tgagcatctt gaactctgct gtggtaaggc
      361 aaggctggtg ctgacgaagg acggaagcat atttctcaac ggtacgcaca ttcatctgga
      421 aggggagtcg gatgtgaacg gtgatgcgcc agtgattaac tggaactgtg gtgccacaca
      481 acctgtaccg gatgcgcctg tgccgaaaga tttacccccc ggaatgccgg atatgcggca
      541 attttgagtc aggtgcaaaa aattaaaacg gaactggtgc agtgaccaga tgaaccatat
      601 taaaagcaga aggaattgat tcatgggc
//
LOCUS       DAZPLI010000438          624 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZPLI010000438 DAZPLI010000000
VERSION     DAZPLI010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.441"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            1..424
                     /locus_tag="ACHUU3_004625"
                     /pseudo
     CDS             1..424
                     /locus_tag="ACHUU3_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
BASE COUNT          213 a          108 c          120 g          183 t
ORIGIN      
        1 tgataaagaa aattcactta agcgtgtcat taatagtttt aattctgaac tcatgggata
       61 aatgtcgaat aataatataa aagttcattt aaatgaacca gagataatat ttttacatgc
      121 cgttcttcag caatacttaa gccaaagttg cggtgcgttt gtgtgcatgg cagcccagga
      181 agtgattgaa caaagggaaa gcaattctga cagcgccccc tatacgttat taaaaaacta
      241 tgctgacaga tttaaaaaat attcagcaga agagcagtac gaaattgatt tttaacatcg
      301 actggtaaac aggaattgtt atttagataa atatggcgat gcaaatatca atgattatta
      361 tagagactta gaaataaaac actcacaacg caaaaataga gcatcaggaa aaagagtaag
      421 ttaacatcat tgttaattaa ttcgaccagt cagcaaatat gctgactggt cgaacatagt
      481 ttgctaaaca gtcatcactg actgatgtca gtcacaccat ccgccagttc aatcttcggg
      541 tatttccagc gtatctcggc acgcgattgc tggtctgttg atgtagtgga ttgttgttgc
      601 tgacttttct taccctcaga gttc
//
LOCUS       DAZPLI010000439          614 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZPLI010000439 DAZPLI010000000
VERSION     DAZPLI010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.442"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            68..>614
                     /locus_tag="ACHUU3_004626"
     CDS             68..>614
                     /locus_tag="ACHUU3_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR2084465.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAH"
BASE COUNT          229 a          129 c           85 g          171 t
ORIGIN      
        1 taacaagcta gcatcgctgg atgtctcccg gcaagaatct ctgattgagt taagtgccca
       61 tcacaatatg atcactgacc ttatattaca caatcatccc aggatgaaaa caatcacttt
      121 aaacgacaac catattgcac atttaaacgc caaaaacact acaaaactgg aatatttaaa
      181 cttaagcaat aacaatttac tgccaaccaa tgacattgat caactaatat catcaaaaca
      241 tctttggcat gtattagtta acggcatcaa caatgatcca cttgcacaaa tgcagtactg
      301 gactgcagta agaaatataa ttgatgacac taatgaagtg accattgatt tatcaggact
      361 taatttaacc actcaaccac cagggctgca aaacttcaca tctatcaatc ttgataataa
      421 ccaactcaca cattttgatg caaccaacta cgatagactc gtaaagctaa gtctgaatag
      481 taatgctctt gagtcaataa attttcctca aggcagaaat gtaagtatta cacatatatc
      541 tatgaataat aatgctctca gaaatattga tatagatagg ctttcatcag ttacttattt
      601 tagtgcggca cata
//
LOCUS       DAZPLI010000440          593 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZPLI010000440 DAZPLI010000000
VERSION     DAZPLI010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.443"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..91
                     /locus_tag="ACHUU3_004627"
     CDS             <1..91
                     /locus_tag="ACHUU3_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084466.1"
                     /translation="GLENAPQTFIGLLKGKNFGKVVIRVAGDD"
     gene            289..>593
                     /gene="mcbR"
                     /locus_tag="ACHUU3_004628"
     CDS             289..>593
                     /gene="mcbR"
                     /locus_tag="ACHUU3_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR2084467.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAV"
BASE COUNT          172 a          121 c          149 g          151 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctgt tga
//
LOCUS       DAZPLI010000441          590 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZPLI010000441 DAZPLI010000000
VERSION     DAZPLI010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.444"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            1..248
                     /gene="tnpA"
                     /locus_tag="ACHUU3_004629"
                     /pseudo
     CDS             1..248
                     /gene="tnpA"
                     /locus_tag="ACHUU3_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          185 a          137 c          112 g          156 t
ORIGIN      
        1 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctatcctccc aaactggcga
       61 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gtgactgcga cgagatcgac
      121 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtgttggc tattttgcca
      181 gtagctgcgg aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
      241 ctggttaggt ggaaaaccac gccttatatc cctgccctga aggacggggt tttacggcgc
      301 accggataag cctggagatt atctacacca gcatggacat cattgtacta tgccccgtac
      361 atgccccatc acatcctcaa catcaaagta ttagagcctt gcaaaccatt atgcgtctca
      421 gtttgtctca tctatcttac gcaaaaaaaa tagaactaat atcaagcggc gctatattgc
      481 gtattaaata aaaccacctc aattacatta ataaatctaa acaaacatca tgcaaataat
      541 ttccatggtt atttctaggc tatttaaatt aaataaatta tgagtcaatc
//
LOCUS       DAZPLI010000442          560 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZPLI010000442 DAZPLI010000000
VERSION     DAZPLI010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.445"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..>560)
                     /locus_tag="ACHUU3_004630"
     CDS             complement(<1..>560)
                     /locus_tag="ACHUU3_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFR2084468.1"
                     /translation="VWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGFI
                     GRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSAD
                     LFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLW
                     QDEHLLRLCLRQALQLADVVKLSEEE"
BASE COUNT          146 a          167 c          128 g          119 t
ORIGIN      
        1 attcttcttc cgagagcttg acgacatccg ccagttgtag cgcctgccgc aaacacaagc
       61 ggagcaaatg ctcgtcttgc catagatctt cacgaatatt aggatcgaag ctgacaaaac
      121 ctccggcatg ccggatcgcc gtcatcgcag taaatgcgct ggtacgcgaa ggctcggcag
      181 acaacgcaat tgaacagaga tgtaaccatt cgccatgtcg ccagcagggc aagtctgtcg
      241 tctctaaaaa aagatcggca ctggggcgga ccataaacgt aaatgaacgt tccccttgat
      301 cgttcagatc gacaagcacc gtggatgtcc ggtgccattc atcttgcttc agatacgtga
      361 tatcgactcc ctcagttagc agcgttcttt gcattaacgc cccaaaagga tcatcaccga
      421 cccgacctat aaatccactt attccgccta atctggcgat tcccaccgca acgttagctg
      481 gcgcgccgcc aggacaaggc agtaggcgcc cgtctgattc tggcaagaga tctacgaccg
      541 catcccctaa aacccatact
//
LOCUS       DAZPLI010000443          556 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZPLI010000443 DAZPLI010000000
VERSION     DAZPLI010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.446"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            78..556
                     /locus_tag="ACHUU3_004631"
                     /pseudo
     CDS             78..556
                     /locus_tag="ACHUU3_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
BASE COUNT          136 a          121 c          123 g          176 t
ORIGIN      
        1 agctgataga aacagaagcc actggagcgc ctcaaaaaca ccatcataca ctaaatcagt
       61 aagttggcag catcaccgcc gttgtctaca catttattct ctcggtgctg atttaccggg
      121 aagtaaaatg gcgccattta cccaaactga ttctggagtc ggttgttacc acttcgattg
      181 tattactgtt gatcggtttt tccgtgggca tgtcctgggc aatgactaat gctgatattc
      241 cttatatgat cagcgacgca ttgatgggga tttctgataa cccactgatc atattgctgc
      301 tgattaatat cgtcttatta attgtcggca tctttatgga catgacaccg gctgtactaa
      361 tctttacacc gatcttcttg ccgattgcac aagagctggg aatggaccca gtacatttcg
      421 gtattatgat ggtggccaac ctttgtattg ggctgttaac accgccggtg ggtagtgcgc
      481 tatttgttgg ttgttctatc tcgggtgtga agattcaaca tttaatcaaa ccattgctgc
      541 cattttatgc tagcat
//
LOCUS       DAZPLI010000444          556 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZPLI010000444 DAZPLI010000000
VERSION     DAZPLI010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.447"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>556
                     /gene="feaR"
                     /locus_tag="ACHUU3_004632"
     CDS             <1..>556
                     /gene="feaR"
                     /locus_tag="ACHUU3_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFR2084469.1"
                     /translation="WQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDAS
                     RPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNN
                     PALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWI
                     AGETGMSVRSLYRMFADKGLVVAQY"
BASE COUNT          142 a          125 c          146 g          143 t
ORIGIN      
        1 tggcaggaag taaaaggcag cgacgatgcc tggttttaca ccgtttttca gcttagtggt
       61 caggcaataa tggagcagga tgagcgtcag gtgcagattg gcgctggcga tattacgtta
      121 ctcgatgcct cacgcccctg ttcgctttac tggcaggagt cttctaaaca gatttcatta
      181 cttttgccac gcactctgct ggaacaatat tttccccatc aaaaacctgt ctgcgcagaa
      241 agactggacg ctgacttacc catggtgcaa ctcagtcatc gcctgttaca ggagagcatg
      301 aataatccgg cactttctga aacagaaagt gaagctgcgc tacaggcgat ggtgtgtctg
      361 ctgcgcccgg tacttcatca gcgggaatct gttcaacctc gccgtgaacg tcagtttcaa
      421 aaagtggtta cgttgataga cgataatatt cgcgaagaga tattacgccc ggagtggata
      481 gccggagaga caggtatgtc agtacgtagt ttgtaccgaa tgtttgccga taaaggtttg
      541 gtagtcgcgc aatata
//
LOCUS       DAZPLI010000445          550 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZPLI010000445 DAZPLI010000000
VERSION     DAZPLI010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.448"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>550
                     /locus_tag="ACHUU3_004633"
     CDS             <1..>550
                     /locus_tag="ACHUU3_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase"
                     /protein_id="HFR2084470.1"
                     /translation="IISALYFLITPKKTITNNPTLLIFISLCLLGIINIIWYSHYKVS
                     GSVYTNAYRGPMETGKIALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAH
                     AMWQHFYLNVDRVALSASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSL
                     CAVIVTETRAAILVFPFFALLLI"
BASE COUNT          161 a          110 c           79 g          200 t
ORIGIN      
        1 attatatctg cattgtattt tctaataaca ccaaaaaaaa caataactaa caatcctact
       61 ttgttaattt tcatctcatt atgtcttttg ggaatcataa atatcatctg gtattcacat
      121 tataaagttt caggctctgt ttataccaat gcatatcgtg gcccaatgga aactgggaaa
      181 attgccttgt gtagtgcttt tattttcttg gttctttttg ctaaaaatga gttgagaaca
      241 aaaataaaat ttgggaaact aattctgttc gcatccctgg caacgcagtt actttttttt
      301 gcgcatgcca tgtggcaaca tttctattta aacgttgacc gtgttgcatt atcagcttcc
      361 cacgctacaa cagcaggcta catcatcctt ttcccttctt tactggcatc aattctcatt
      421 ttaaaatccg actttagaca taaaacaaca ttatatacaa ttaacttcat gcttagctta
      481 tgtgctgtca tagtaactga aacgcgtgca gccatattag tgtttccatt ctttgcgtta
      541 ctattaatcg
//
LOCUS       DAZPLI010000446          546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZPLI010000446 DAZPLI010000000
VERSION     DAZPLI010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.449"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            1..546
                     /locus_tag="ACHUU3_004634"
                     /pseudo
     CDS             1..546
                     /locus_tag="ACHUU3_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
BASE COUNT          194 a          120 c           83 g          149 t
ORIGIN      
        1 ccgacttatt aaaaatgcct gtttaaaatt atataattta gcacataccg caaccaactc
       61 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
      121 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
      181 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg
      241 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
      301 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
      361 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
      421 agtccagtta ttccccataa atactggcgg acactggata ttagtttcgc tacagaaaat
      481 agtaaataaa aaaataataa actacaaata aaatgcgtca tattcaactc attgcgtgca
      541 ctaggc
//
LOCUS       DAZPLI010000447          546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZPLI010000447 DAZPLI010000000
VERSION     DAZPLI010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.450"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            1..546
                     /locus_tag="ACHUU3_004635"
                     /pseudo
     CDS             1..546
                     /locus_tag="ACHUU3_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          159 a          128 c          144 g          115 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgcca
//
LOCUS       DAZPLI010000448          536 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZPLI010000448 DAZPLI010000000
VERSION     DAZPLI010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.451"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..114)
                     /locus_tag="ACHUU3_004636"
     CDS             complement(<1..114)
                     /locus_tag="ACHUU3_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084471.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFA"
     gene            complement(370..>536)
                     /gene="tdcR"
                     /locus_tag="ACHUU3_004637"
     CDS             complement(370..>536)
                     /gene="tdcR"
                     /locus_tag="ACHUU3_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFR2084472.1"
                     /translation="AARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSSDR
                     TSFCECNRFP"
BASE COUNT          163 a          103 c          105 g          165 t
ORIGIN      
        1 tgcaaacttt ctttttaccg tccaataatc aatatttcta ttgtgattgt taaccactgc
       61 gtgcattggc gggattgaag ggtgaaaaat atgtgcaatt gggaagcctt tcattgatgc
      121 ctgccttgtc gtcatgacaa acaataatag atgcttattt tcctcgcagg gttctttcgc
      181 actatcaaaa aacgctcatt tacccggcgt tacagtaaac gaagcaacat tctgtggcct
      241 acgacgatca aaagctctgt gaaaatttcc cttgttgtct ttgtctgacc ttatcgcggc
      301 gatcagcaat tattcgtttt gtgatcctca acacaaaatg ctattcgtcg cctatgaggg
      361 gagaagaatt tatggaaaac gattgcattc acaaaatgac gtcctgtcag aagagaaaaa
      421 gttgacgtta atcaattgtt cgagatgatc aatacctgta ttcgaaattt taaaatattg
      481 ataaaataaa tgtttgttct ccaacaaata gcgtgaaaaa atgtgacgtg ccgcag
//
LOCUS       DAZPLI010000449          521 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZPLI010000449 DAZPLI010000000
VERSION     DAZPLI010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.452"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..>521)
                     /locus_tag="ACHUU3_004638"
     CDS             complement(<1..>521)
                     /locus_tag="ACHUU3_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084473.1"
                     /translation="FLGVTTWIFMAAGGAESVAVYVNDVKGGSKSFVKVIILAGIFIG
                     VLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNRFVGLVSFTAMFGS
                     LLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIVIPLMIIPMLGSNT
                     VQDLMNTIINMTA"
BASE COUNT          143 a          141 c          132 g          105 t
ORIGIN      
        1 cggcggtcat attaataata gtattcatca gatcctgcac ggtattggaa ccgagcatcg
       61 ggataatcat cagcgggatg acgatcagga actggatcca cgctgcgcgc gccggaacgc
      121 cgttttcgtt cagttcgacg gttttcttac caaagatgcc ttccgggatt tcggagaaga
      181 aaattttcac cggcgttgcg gtccacatca gcagggaacc gaacatcgcg gtaaaggaca
      241 ccagaccgac aaagcgattc atcaacgctt ccggtagacc aaaatacgcc gccatgccgt
      301 ggaatacctg caccgatccg ccggtaaatt tcaactcttt gctgctgacg aagacgttaa
      361 tcagcaccga ggagacggaa tacagtacgc cgataaaaat tccggcgagg atgatcactt
      421 taacgaacga tttcgaaccg cctttgacgt cgttaacgta cacagcgacg gactccgcgc
      481 cacctgcggc cataaagatc caggtggtaa cgccgaggaa c
//
LOCUS       DAZPLI010000450          513 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZPLI010000450 DAZPLI010000000
VERSION     DAZPLI010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.453"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..487
                     /gene="espX1"
                     /locus_tag="ACHUU3_004639"
     CDS             <1..487
                     /gene="espX1"
                     /locus_tag="ACHUU3_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFR2084474.1"
                     /translation="ITIEKSNLSYGYYFCCVLTNISCFESDLSNTIFSNGEINNLFIK
                     KSNIFGTSFTNTMIKNLRCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFE
                     ILIPLVQTLVRDNVKLNNDVYKELNKFMHDYDKTSSKMRKYLQSINECMLLIKNIVHQ
                     D"
BASE COUNT          199 a           88 c           79 g          147 t
ORIGIN      
        1 tataacaata gaaaagagca acctttcata tgggtattat ttttgttgtg tattaaccaa
       61 tatatcatgt tttgaaagcg acttatcaaa caccatcttt tcaaatggag aaataaacaa
      121 cctcttcatt aaaaaaagca acatatttgg aacatcgttc actaatacca tgataaaaaa
      181 tttgcgctgt gaagatatta tgcctggccg atggaccact caacttgtca ataagcactt
      241 aggttacaga tacacaggag tattcaaaac cctggcaagt attgatgaca aaccgtcccg
      301 tttcgaaatt ttgataccat tggtacaaac tttagttcgc gataatgtaa agctaaataa
      361 tgatgtgtat aaagaactta ataagtttat gcacgattac gacaaaacat catcaaaaat
      421 gagaaaatac cttcagagca tcaacgagtg catgctcctt ataaaaaata tagttcatca
      481 ggattaaaac actcaaagaa agaggtaatt tta
//
LOCUS       DAZPLI010000451          511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZPLI010000451 DAZPLI010000000
VERSION     DAZPLI010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.454"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>511
                     /locus_tag="ACHUU3_004640"
     CDS             <1..>511
                     /locus_tag="ACHUU3_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084475.1"
                     /translation="EGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAYSE
                     QGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAYEL
                     ATAWNTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTT
                     AANLDIDIP"
BASE COUNT          109 a          147 c          187 g           68 t
ORIGIN      
        1 gtgaaggcca ccgtgtggcg gtccactacg gctatgacga taaaggccgt ctgacgggcg
       61 aacggcagac ggtggagacc ccggagacgg gggagatgct gtgggagcat gagacggggc
      121 acgcgtacag cgaacagggg ctggcgaccc gtcaggagcc ggacggcctg ccgccggtgg
      181 agtggctgac gtatggcagc ggttatcttg cggggatgaa gctgggcgga acaccgctgg
      241 tcgagtacac gcgggaccgg ctgcaccgtg agacggcccg cagcttcggc ggggaggcat
      301 atgaacttgc caccgcctgg aataccagcg gccagctccg gagcaggcac ctgaaccttc
      361 cgcagcttga ccgtgactac gactggaacg acaacggaca gctaatccgc atcagtggcc
      421 cgcaggagag ccgggagtac cgttacagcg acacggggag gctgacgggc gtccacacca
      481 cggcagcgaa cctggatatc gatatcccgt a
//
LOCUS       DAZPLI010000452          503 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZPLI010000452 DAZPLI010000000
VERSION     DAZPLI010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.455"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            53..>503
                     /locus_tag="ACHUU3_004641"
     CDS             53..>503
                     /locus_tag="ACHUU3_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HFR2084476.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQVSIVYEAGPCGYGLYRRLVKSGFDCMVCAPSLIPKKPGERVKTDRRD
                     AIRLVRSLRAGDLSAVYVPGIEDEAFRDLARAWASARDDLRHARQRLK"
BASE COUNT          120 a          122 c          131 g          130 t
ORIGIN      
        1 ccgcatgggt gagctgttgc tacaccccgg tatttccacc acggaggcca gcatgaacaa
       61 caataacaca ttgtacgtcg ggttagatgt tcacaaagaa tcgattactg tcgcttatgc
      121 tatcaattca gaacctgttg aactgatggg taaaattggc acatcaccta ctgatattca
      181 gaatctttgt aaacgtctca ggtcaaagtc atcgcaggtc agtatcgttt atgaagcggg
      241 gccctgtggt tatggacttt atcgtcggct ggtgaaatcc ggttttgact gcatggtctg
      301 cgccccctca cttatcccga aaaaaccggg ggagcgggtt aaaaccgatc gccgtgatgc
      361 catcagactt gtgcgttcac tgcgtgcagg agatctttct gctgtctacg tacccggcat
      421 tgaagatgag gcattccggg atttggcccg ggcatgggca tctgcccgcg atgatttacg
      481 gcatgcaagg caacgtctga aat
//
LOCUS       DAZPLI010000453          490 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZPLI010000453 DAZPLI010000000
VERSION     DAZPLI010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.456"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(76..423)
                     /gene="tnpB"
                     /locus_tag="ACHUU3_004642"
     CDS             complement(76..423)
                     /gene="tnpB"
                     /locus_tag="ACHUU3_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR2084477.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          111 a          136 c          134 g          109 t
ORIGIN      
        1 atgtcgtcag aagagatgtc gttcataagc ccgtatttta ccgggcttat tctgtgacaa
       61 ccaggataaa gagatttaca gcatggtcag ggaggtcagc agccgcttag gctgtcgcca
      121 gtcgatacct tccagcagca tcgccagctg cgcctgtgta aggaacactt tgccatcacg
      181 ggctgacggc caggcgaagc gcccacgctc cagccgtttg gtcaggaggc acagtccgtc
      241 actggtggac cacagcagtt taacctgact gccgctgcgg ccccggaaaa tgaaaacatg
      301 gccggacatg ggatcgtctt tcagcgccgt ctgtactttc gcagccaggc cgttgaagcc
      361 atttctcata tcggtgatac cggcaaccag ccaaattttg atcccggaag gtaacgggat
      421 catcgcttca gttcctgtat cagcagagtc aggagctttt cgctgacatt gccattgaag
      481 cggagcgtcc
//
LOCUS       DAZPLI010000454          489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZPLI010000454 DAZPLI010000000
VERSION     DAZPLI010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.457"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUU3_004643"
     CDS             complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUU3_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR2084478.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRT
                     EADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTD"
BASE COUNT           99 a          153 c          117 g          120 t
ORIGIN      
        1 gtcagtcagc tgacggtaaa tctgctgttc agtctcacgc atcacctgtg caccggcttc
       61 cctctccgca tccgcatcac cgctcagacc tgatgctttc agccggtcag ccaccctctg
      121 agggtactca ttctccagca tctcatattt ctgctcttct gccagcgccc agcggtcagc
      181 ttccgtacgc ttcagtacag catgccatgg tccccagagg gagaaccagt ccgtaaattc
      241 attctcttca cggcttctga ccatggcttc ggcagtgcgg aggtcatttg ctgtcactcc
      301 cgacacgcca tagaaacgca tttccttcac ggcagtggag agctgaagtt tctctgcgag
      361 catggtctgg aaggccaggt agacttctat ctcatccaca aaatggagag ttctgacttt
      421 atcccgggca atgtcctcca gaatttcgag gcggaacatt tccctgccca gggagagcag
      481 agcgccggt
//
LOCUS       DAZPLI010000455          480 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZPLI010000455 DAZPLI010000000
VERSION     DAZPLI010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.458"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>480
                     /locus_tag="ACHUU3_004644"
     CDS             <1..>480
                     /locus_tag="ACHUU3_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR2084479.1"
                     /translation="SQPLQQSLKDLERAYKNFFQNRAAFPRFKKRGQNDVFRYPQGVK
                     LDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVHPSA
                     SMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRK"
BASE COUNT          138 a          119 c          123 g          100 t
ORIGIN      
        1 cgtcacagcc attgcaacag tcactgaaag accttgagcg ggcttacaaa aacttcttcc
       61 agaatcgggc tgcttttccc cgattcaaaa agcggggaca gaatgatgta ttccgctacc
      121 cgcagggcgt taagctcgat caggaaaaca gccgtatttt tctgccgaaa ctgggctgga
      181 tgcgctaccg gaacagccgt caggtcacgg gtgttgtgaa aaatgtcact gtcagccagt
      241 cctgcggtaa gtggtacatc agtattcaga cagaaagtga agtatcaact ccggttcacc
      301 cttcagcatc aatggtcggg ctggatgctg gcgtggctaa actcgccacg ctgtcagatg
      361 gcacagtctt tgagcctgta aacagttttc agaaaaacca gaagaagctg gcgagacttc
      421 agcgacagtt aagccgcaag gtcaaattca gcaacaactg gcagaagcag aaacgcaaaa
//
LOCUS       DAZPLI010000456          476 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZPLI010000456 DAZPLI010000000
VERSION     DAZPLI010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.459"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>476
                     /locus_tag="ACHUU3_004645"
     rRNA            <1..>476
                     /locus_tag="ACHUU3_004645"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZPLI010000457          468 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZPLI010000457 DAZPLI010000000
VERSION     DAZPLI010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.460"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            85..444
                     /locus_tag="ACHUU3_004646"
                     /pseudo
     CDS             85..444
                     /locus_tag="ACHUU3_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
BASE COUNT          114 a          109 c          137 g          108 t
ORIGIN      
        1 gtggtgatgt ctatctcaca ccgatgaaca tgaccacgaa accctccgat ggcagtaagg
       61 ccggtaagca gaaggataac gccaatgcag acgaaacaac gtcttgatgt accgctgagt
      121 ctgaaatctg tcagtgactc cggtgagttt gaagggtatg gctccgtctt tggtgtaaag
      181 gacagccacg atgatgtggt gatgtccggg gcatttgctg cttccctgcg ggcgtggagt
      241 gacagaaaag cgttacctgc gctgctctgg cagcaccgca tggatgaacc catcggtgtt
      301 tacaccgaaa tgaaggaaga cgatgtcggg ctttacgtca ggggacggtt gcttattgat
      361 gatgatcccc tcgcaaaacg tgcacatgca cacatgaagg ccggttcgtt aaccggcctt
      421 tctattgggt acgtcctgaa agactgaggt agcctgagtt taacggac
//
LOCUS       DAZPLI010000458          465 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZPLI010000458 DAZPLI010000000
VERSION     DAZPLI010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.461"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>465
                     /locus_tag="ACHUU3_004647"
     CDS             <1..>465
                     /locus_tag="ACHUU3_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR2084480.1"
                     /translation="VKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTG
                     GFNSETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTA
                     VRSPESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSAL"
BASE COUNT          119 a          108 c          150 g           88 t
ORIGIN      
        1 gtgaaagaaa gtaatcagcg atggtgctct gacgggttcg agttccgctg tgataacgga
       61 gaaaaactgc gagtcacgtt cgcgctggac tgctgtgacc gtgaggcact gcactgggcg
      121 gtcactacgg gcggcttcaa cagtgaaaca gtacaggacg tcatgctggg agcggtggaa
      181 cgccgcttcg gcaacgagct tccggcgtct ccagtagagt ggctgacgga taatggttca
      241 tgctaccggg ctaatgaaac acggcagttt gcccggatgt tggggcttga accgaagagc
      301 acggcggtgc ggagtccgga gagtaacggc atagcagaga gcttcgtgaa aacgataaag
      361 cgtgactaca tcagtgtcat gcccaaacca gacgggttaa cggcagcaaa gaaccttgca
      421 gaggcgttcg agcattataa cgaatggcat ccgcatagtg cactg
//
LOCUS       DAZPLI010000459          461 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZPLI010000459 DAZPLI010000000
VERSION     DAZPLI010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.462"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>461
                     /locus_tag="ACHUU3_004648"
     CDS             <1..>461
                     /locus_tag="ACHUU3_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR2084481.1"
                     /translation="ESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGF
                     NSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVR
                     SPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSAL"
BASE COUNT          117 a          108 c          146 g           90 t
ORIGIN      
        1 aagaaagcaa tcagcgatgg tgctctgacg ggttcgagtt ctgctgtgat aacggagaga
       61 gactgcgtgt cacgttcgcg ctggactgct gtgatcgtga ggcactgcac tgggcggtga
      121 ctaccggcgg cttcaacagt gaaacagtac aggacgtcat gctgggagcg gtggaacgcc
      181 gcttcggcaa cgatcttccg tcgtctccag tggagtggct gacggataat ggttcatgct
      241 accgggctaa tgaaacacgc cagttcgccc ggatgttggg acttgaaccg aagaacacgg
      301 cggtgcggag tccggagagt aacggaatag cagagagctt cgtgaaaacg ataaagcgtg
      361 actacatcag tatcatgccc aaaccagacg ggttaacggc agcaaagaac cttgcagagg
      421 cgttcgagca ttataacgaa tggcatccgc atagtgcgct g
//
LOCUS       DAZPLI010000460          456 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZPLI010000460 DAZPLI010000000
VERSION     DAZPLI010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.463"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            2..388
                     /locus_tag="ACHUU3_004649"
     CDS             2..388
                     /locus_tag="ACHUU3_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR2084482.1"
                     /translation="MKGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDV
                     YNSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNII
                     CSECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          135 a           95 c          122 g          104 t
ORIGIN      
        1 aattaaaggg caggttgttg aacttgtacg tctgagcggt cgcgagacgt tgcggcaact
       61 ggaagccaag acaggtgcga caagatatct gatgagtgtt ctcgccagag agctggttgc
      121 cagtggcgat gtatacaact ctggttacgg gttattcccg tctgaacagg cgcgtaagga
      181 ctggcaaaac gcccgcaaaa aactctcaag ggcaaatctg aagaaaccat ctgtggttga
      241 tcctgacctt atctggtcat taccagacgg agaaatacgc cgctacgaca ggcgtcaaaa
      301 cataatctgt agcgagtgcc ggaagagcga agctatgcag cgtgtactgg ctttctatca
      361 gggtaatttt cagaaggtgc tgttgtgagc caaattaaca atcggaactt cgtgaagaga
      421 aagcataatc caaaatctga ataattaagt tcagca
//
LOCUS       DAZPLI010000461          447 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZPLI010000461 DAZPLI010000000
VERSION     DAZPLI010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.464"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(72..>447)
                     /locus_tag="ACHUU3_004650"
     CDS             complement(72..>447)
                     /locus_tag="ACHUU3_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084483.1"
                     /translation="TSADNDVDLDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIG
                     VFDFANNASPATMTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVN
                     VNGESGVTYENCKLNMKDINDD"
BASE COUNT          138 a           99 c           70 g          140 t
ORIGIN      
        1 gagaaggtaa agaaggtaag tccatactgt ctccactata aaaaaaccgc caatttctgg
       61 cggcttatat attaatcatc attaatatct ttcatattca atttgcaatt ttcgtaggtc
      121 actcccgact cgccattaac attaactcca gctctgatat tcccgttttc ttttacatct
      181 acaaaaaact ggcctcttgg catcttgaac gcaaatgtaa aaccaacagt atctgaatca
      241 aaatcatcat ctttatatat tgggatcaaa aatgtcatcg tcgctggtga tgcattgttt
      301 gcaaaatcaa atacacctat acttttgccg aattcataag aagcttttct caatataatc
      361 gcaccgctgg cagaagggga tgggcacgat atcctcagtt gtctgaatcc atctgtatcc
      421 aagtcaacgt cattatctgc agatgtt
//
LOCUS       DAZPLI010000462          436 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZPLI010000462 DAZPLI010000000
VERSION     DAZPLI010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.465"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>436
                     /locus_tag="ACHUU3_004651"
     CDS             <1..>436
                     /locus_tag="ACHUU3_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR2084484.1"
                     /translation="WHIAANIQFPPNFDESKQYPAIISVHPFGSCKEQTSGNIYGKAL
                     AEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLPYVNAERIGVLGV
                     CGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
BASE COUNT          108 a           84 c          123 g          121 t
ORIGIN      
        1 tggcatattg ctgccaatat tcagtttcca ccaaattttg atgagtcaaa acagtatccg
       61 gcgattatca gcgtccatcc ttttggcagt tgtaaggagc agacgtctgg caatatctat
      121 ggcaaggcat tggctgaaaa aggctatctc gttctcgctt atgacgccag tttccaggga
      181 gaatctggtg gggagcctcg ctggatagaa gatccgacac agcgcgtaga ggatattagc
      241 cgtgttatcg attatgcagt aactctgcca tatgttaatg ctgaacgtat tggcgtgctg
      301 ggagtgtgtg ggggtggtgg ctatgccatt aatgccgcgc tgacagaaaa acgaattaaa
      361 gcagtagtga gtattacggg tgttaatatc gggcgtcttt ttcgggaagg tttcagcaat
      421 tacgatccga ttggcg
//
LOCUS       DAZPLI010000463          427 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZPLI010000463 DAZPLI010000000
VERSION     DAZPLI010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.466"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>427
                     /locus_tag="ACHUU3_004652"
     CDS             <1..>427
                     /locus_tag="ACHUU3_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HFR2084485.1"
                     /translation="IVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQF
                     QPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQK
                     RKEGLSIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA"
BASE COUNT          101 a          105 c          128 g           93 t
ORIGIN      
        1 atcgtcgact ttgactctga caaggcctcc agtgcggccc gtgcggcatg gggtaactcc
       61 tcctacaaaa ttatcctcaa acagagtgcg aaggagttcg caaaatacaa ccagctgtat
      121 ccggaccagt tccagccact gcagcgcgac atgattggta agtttggtgc ggccaaagac
      181 cagtggttca gttccttcct gctgcaggtg gaaaaccatt cctcctggca ccgcctgttt
      241 gtggacccgt taagccgcgc catgtacagc tctgacggcc cggattttga gtttgtgcag
      301 cagaaacgta aagaggggct gagtattcat gaggcggtgt ggcagctggc gtggaagaag
      361 tcagggccgg agatggcttc gctggaagcc tggctggaag aacatgagaa atacaggagt
      421 gttgcat
//
LOCUS       DAZPLI010000464          426 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZPLI010000464 DAZPLI010000000
VERSION     DAZPLI010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.467"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..>426)
                     /locus_tag="ACHUU3_004653"
     CDS             complement(<1..>426)
                     /locus_tag="ACHUU3_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR2084486.1"
                     /translation="TFLKSPYINDVRILDWCFNKRMQYFGDSEKIKYACSVINHIDFS
                     CDQSKIKIAETLFFNLDKEPYKNSRKLQELIWDKLVAYVNDFSLSNQEKSRLILRLFD
                     DVKLLFNEVPVSILVNDIFLKDFFMKQPDFAKWYFYQLL"
BASE COUNT          155 a           71 c           52 g          148 t
ORIGIN      
        1 ttaagtaatt gatagaagta ccacttagca aaatcaggtt gcttcatgaa aaaatccttc
       61 aaaaaaatat catttactaa aatgctgaca ggcacttcat taaatagtaa cttaacatca
      121 tcaaataacc ttagtattaa gcgtgatttt tcttgattgc ttaagctaaa atcattgaca
      181 tatgcaacca acttatccca aatcaattcc tgtaatttac ggctattttt atagggttct
      241 ttatcgagat tgaaaaataa ggtctcagct attttaattt tagattgatc gcagctaaaa
      301 tcaatatgat ttatcacgga gcatgcatac tttatctttt cagaatcacc aaaatattgc
      361 attcttttat taaaacacca atccagtatt cttacatcat taatatatgg ggattttaaa
      421 aaagtc
//
LOCUS       DAZPLI010000465          423 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZPLI010000465 DAZPLI010000000
VERSION     DAZPLI010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.468"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            77..>423
                     /locus_tag="ACHUU3_004654"
     CDS             77..>423
                     /locus_tag="ACHUU3_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR2084487.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNL"
BASE COUNT          131 a           70 c          107 g          115 t
ORIGIN      
        1 caggttaaaa actttcctgt tttcaacggg gctctcccgc tgaatattcg cgcgttaatt
       61 aaaatcagga atgaaaatga aaaagagcac tctggcatta gtggtgatgg gcattgtggc
      121 atctgcatct gtacaggctg cagaaatata taataaagac ggtaataaac tggatgtcta
      181 tggcaaagtt aaagccatgc attatatgag tgataacgcc agtaaagatg gcgaccagag
      241 ttatatccgt tttggtttta aaggtgaaac acaaattaac gatcaactga ctggttatgg
      301 tcggtgggaa gcggagtttg ccggaaataa agcggagagt gatactgcac agcagaaaac
      361 gcgtctcgct tttgccgggt tgaaatataa agatttgggt tctttcgatt atggtcgtaa
      421 cct
//
LOCUS       DAZPLI010000466          422 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZPLI010000466 DAZPLI010000000
VERSION     DAZPLI010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.469"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..419)
                     /locus_tag="ACHUU3_004655"
     CDS             complement(<1..419)
                     /locus_tag="ACHUU3_004655"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR2084488.1"
                     /translation="MKTNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEHLLPH
                     FLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHKISHD
                     VFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIR"
BASE COUNT          125 a           85 c           87 g          125 t
ORIGIN      
        1 ttgcgtatta cacagctttt tgtagatata tttccaggca ggcagtgaag aaggtaaaac
       61 atggaaatgg aatcaaattg accatgtaac gccgcgggat aaggttcaaa aacatcatgg
      121 ctaattttat gtttaatttt tgattcccca gcccttgtag atgccgcgtt caggctagct
      181 tcgttcaaat ccattaaaga tatcagacta ctctcaggta cgtgagtaag gtaaaaccca
      241 gttccaacac caatatccag atggttgtta cctaaatgtt ccagaaagtg tggaagaagg
      301 tgttcctttg taggacatcc ccatgcaagc cgatttgata ctcccaaaac ccaccagtca
      361 taaagcttta gggtaagtgg tgtgtaaatt ttagccccat catctgtgtt tgttttcatt
      421 ga
//
LOCUS       DAZPLI010000467          420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZPLI010000467 DAZPLI010000000
VERSION     DAZPLI010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.470"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            41..116
                     /locus_tag="ACHUU3_004656"
     tRNA            41..116
                     /locus_tag="ACHUU3_004656"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Ile,seq:cat)
BASE COUNT          117 a           79 c           98 g          126 t
ORIGIN      
        1 tttattgcgt ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt
       61 agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc
      121 attatttacg tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt
      181 catgactaac gccatttcaa ggaagaagtg cggtgggtgg tttcgaatga gggctggtgt
      241 tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct
      301 ggaaaaaatg cctgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa
      361 cgataaagcc agagtattat catttattat accccttcat ttaatcaggt taattactca
//
LOCUS       DAZPLI010000468          412 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZPLI010000468 DAZPLI010000000
VERSION     DAZPLI010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.471"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(116..>412)
                     /locus_tag="ACHUU3_004657"
     CDS             complement(116..>412)
                     /locus_tag="ACHUU3_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HFR2084489.1"
                     /translation="ALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLIS
                     IPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
BASE COUNT          123 a          111 c           98 g           80 t
ORIGIN      
        1 tgcgccagtg gcaatatgtg gcttagctca tgaaaggagc gcaacaagat gatgaatcaa
       61 catataacaa catcttaaaa aaaggcctga cattacgcca ggccttctgc gttaattaat
      121 cacgcttttc cagcagggtc cggtaaatca gaccaccgat aatgccgccg acaattggca
      181 ccacccagaa gaaccacagt tgttctaatg cccagccgcc ctggaagata gcaaccgcgg
      241 tgctgcgcgc cgggttaaca gaagtgttag tcaccggaat actaattaag tgaatcaggg
      301 ttaaggccag accaatagcg atcggcgcaa aacctgccgg cgcgaatttg tcggttgcgc
      361 cgtggatcac caacaggaaa cctgcactca ataccagttc aactaccagc gc
//
LOCUS       DAZPLI010000469          408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZPLI010000469 DAZPLI010000000
VERSION     DAZPLI010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.472"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            54..130
                     /locus_tag="ACHUU3_004658"
     tRNA            54..130
                     /locus_tag="ACHUU3_004658"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="ACHUU3_004659"
     tRNA            173..248
                     /locus_tag="ACHUU3_004659"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          115 a           86 c           97 g          110 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcg
//
LOCUS       DAZPLI010000470          399 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZPLI010000470 DAZPLI010000000
VERSION     DAZPLI010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.473"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>399
                     /locus_tag="ACHUU3_004660"
     CDS             <1..>399
                     /locus_tag="ACHUU3_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR2084490.1"
                     /translation="QSKSLGESLGGLMSDMSHGVDSLTDISYRVRAFFDEVTLAYRSL
                     NDTQKAVADGLANGLLSALKGLAGIVAVRSGIGAVGGIWNLIRAISTLGNVANTAAGR
                     INSRSTGSVEGGKSRLSVADAITRVMIVGMT"
BASE COUNT          105 a           65 c          110 g          119 t
ORIGIN      
        1 tcaatctaaa agtcttggtg aatctcttgg tggcttgatg agcgatatgt cgcatggggt
       61 cgattcacta accgatatta gttacagggt acgagcgttc tttgatgagg tgacattagc
      121 ttatcgttca ctaaatgaca ctcaaaaagc tgtagctgat ggtcttgcaa atggcctgtt
      181 aagtgcatta aaggggcttg ctggtatagt cgctgtacgg tctgggattg gtgctgtagg
      241 tggtatctgg aacttaatta gggccatttc cacgcttgga aatgtggcaa atacagcagc
      301 aggaagaatc aattcacgtt ctactggtag tgttgaaggt ggaaaaagta ggctatctgt
      361 agctgatgca attaccagag taatgattgt cggtatgac
//
LOCUS       DAZPLI010000471          397 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZPLI010000471 DAZPLI010000000
VERSION     DAZPLI010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.474"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>397
                     /locus_tag="ACHUU3_004661"
     CDS             <1..>397
                     /locus_tag="ACHUU3_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR2084491.1"
                     /translation="LYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQ
                     DGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQ
                     AGLNTAFEDINWTLSYSLTKNAWQKGRDQM"
BASE COUNT          128 a           85 c           85 g           99 t
ORIGIN      
        1 tctctataac aaatcgctca atgagtcagg cacgaatatt cagttagtgg gttaccgtta
       61 ttcgaccagt ggatatttta atttcgctga tacaacatac agtcgaatga atggctacaa
      121 catcgaaaca caggatggtg ttattcaggt taagccgaaa ttcaccgact attacaatct
      181 cgcttataac aaacgcggga aattacaact caccgttact cagcaactcg ggcgcacatc
      241 aacactgtat ttgagtggta gccatcaaac ttattgggga acgagtaatg tcgatgagca
      301 attccaggct ggtttaaata ctgcattcga agatatcaac tggacgctca gctatagcct
      361 gacgaaaaac gcctggcaaa aaggacggga tcagatg
//
LOCUS       DAZPLI010000472          389 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZPLI010000472 DAZPLI010000000
VERSION     DAZPLI010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.475"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..>389)
                     /locus_tag="ACHUU3_004662"
     CDS             complement(<1..>389)
                     /locus_tag="ACHUU3_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR2084492.1"
                     /translation="KMPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLW
                     LVRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEV
                     LPAPLPPYRVLTGLVDRFGRTQTFHRE"
BASE COUNT           71 a          134 c          112 g           72 t
ORIGIN      
        1 ttcgcggtgg aacgtctgtg tgcgcccgaa gcggtccacc agcccggtca gtacccggta
       61 cggcggcagc ggcgcaggca gcacctcatc cgcttccggc acccgctcac accagccgag
      121 cagccaccac ggcccctgcg gactgtttgt cgccagataa cgatgcggac ttaagcggag
      181 ttcttccggc agcgcctgcc agagtgcggc cagccggtga ccttcatcca gtttcgccac
      241 gccgccgcgc accagccaga gtgattcgct gcggctgtaa ccgtcctcac cgggaaacag
      301 gtgctcaaaa tacaggcttc tgccgccgtt atcactgagt atcagtgtgt tatcgcgcag
      361 ctgtaagcgg atatccgcag gcattttcc
//
LOCUS       DAZPLI010000473          376 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZPLI010000473 DAZPLI010000000
VERSION     DAZPLI010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.476"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(134..307)
                     /locus_tag="ACHUU3_004663"
                     /pseudo
     CDS             complement(134..307)
                     /locus_tag="ACHUU3_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           47 a          140 c          117 g           72 t
ORIGIN      
        1 cgcaccttcg gctcccggct gcctggcggc atccggtcgt caggcccgac tccgacaggc
       61 agctgctggc gtacctgcca gtctccgccg aacccgctgg cgcggtttcg ggctgccgct
      121 acggtccgat gcgtgaaaac cccgggcttg cagcgaccgt tccggtcgcg cctcctctgt
      181 cgcgccgtgg cctgcgctcc tggcatcccg gcaaaaggcc gccgggatgt aaggcgcaac
      241 gccgtttcgc aaaccgcgct tcacgtcgtt gcggcgggtc cctgcccgtt gttgtgtccg
      301 gtgccattca cctgtttaag cccctgtcgc cgggggctga tacagccaca acctgccgca
      361 ctgactgtcc agggta
//
LOCUS       DAZPLI010000474          373 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZPLI010000474 DAZPLI010000000
VERSION     DAZPLI010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.477"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..180
                     /locus_tag="ACHUU3_004664"
     CDS             <1..180
                     /locus_tag="ACHUU3_004664"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084493.1"
                     /translation="ESANYIGARSVTAAIKPDIKLQPTRHVKTMDALQTGCVHINYYD
                     QVDSARSQLYQEYFG"
     gene            complement(122..373)
                     /locus_tag="ACHUU3_004665"
                     /pseudo
     CDS             complement(122..373)
                     /locus_tag="ACHUU3_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT          129 a           71 c           66 g          107 t
ORIGIN      
        1 gagtcggcga attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag
       61 ctgcaaccta cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata
      121 aattactatg accaagttga ctctgcgaga agtcaactgt atcaagaata ttttggctaa
      181 aatcaacaca ttcgccactc gttttgagaa aaatcacatt gtgtaaacgt cgacatttaa
      241 atatgacatt atcaactgtt gccttagtaa aattgcaatt ctcaatttct ttacattcga
      301 aagttacatg ttgtagattt gatgataaaa aagacacctt agaaataacg tcacctttaa
      361 aatgacaatt tat
//
LOCUS       DAZPLI010000475          367 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZPLI010000475 DAZPLI010000000
VERSION     DAZPLI010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.478"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>367
                     /locus_tag="ACHUU3_004666"
     CDS             <1..>367
                     /locus_tag="ACHUU3_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR2084494.1"
                     /translation="SRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQ
                     IRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPH
                     EYNGGLPPNESENRYWKNSN"
BASE COUNT          106 a           85 c          107 g           69 t
ORIGIN      
        1 agcaggctca ccatgaaagc gctggaaatg gcatgggaaa cccgtggtaa gcccggcggg
       61 gtgatgttcc acagcgatca gggcagtcat tatacgagca ggcagttccg gcagttattg
      121 tggcgatacc agatcagaca gagtatgagc cggcgcggaa actgctggga taacagccca
      181 atggaacgct tcttcaggag tctgaagaac gaatggatgc cggtggtggg ttacgtaagc
      241 ttcagcgagg cagctcacgc cataacggac tatatcgttg gatattacag cgcactaaga
      301 ccgcacgaat ataacggtgg gttaccccca aacgaatcgg aaaatcgata ctggaaaaac
      361 tctaact
//
LOCUS       DAZPLI010000476          365 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZPLI010000476 DAZPLI010000000
VERSION     DAZPLI010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.479"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>365
                     /locus_tag="ACHUU3_004667"
     CDS             <1..>365
                     /locus_tag="ACHUU3_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR2084495.1"
                     /translation="ECQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNLTAVMFDH
                     VRMSTGNFKDCITEQLELTIDYSDIFGNEDLDGYINNIIKMIDTLPDNAMILKSVLAV
                     KLVMQLKILNIVNKNFIEN"
BASE COUNT          131 a           49 c           62 g          123 t
ORIGIN      
        1 cgaatgtcag attagaaatt caaacttcga taattgttat ctttacgctt cgcacttcac
       61 cagagcagag tttctgtctg ccaaagaaat atcatttatt aaatcgaatt tgacagctgt
      121 tatgtttgat catgtgcgaa tgtcgacagg gaattttaaa gattgcatta cagaacaatt
      181 ggaattaact attgattatt cagatatatt tgggaatgaa gatctcgatg gttatatcaa
      241 taacattata aaaatgattg atacattgcc agataatgca atgatattga aatccgttct
      301 ggcagtaaaa ctggtgatgc aattaaaaat tctcaatatt gttaataaaa actttattga
      361 gaata
//
LOCUS       DAZPLI010000477          356 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZPLI010000477 DAZPLI010000000
VERSION     DAZPLI010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.480"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>356
                     /locus_tag="ACHUU3_004668"
     rRNA            <1..>356
                     /locus_tag="ACHUU3_004668"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           95 a           82 c          105 g           74 t
ORIGIN      
        1 aaaaacacag cactgtgcaa acacgaaagt ggacgtatac ggtgtgacgc ctgcccggtg
       61 ccggaaggtt aattgatggg gtcagcgcaa gcgaagctct tgatcgaagc cccggtaaac
      121 ggcggccgta actataacgg tcctaaggta gcgaaattcc ttgtcgggta agttccgacc
      181 tgcacgaatg gcgtaatgat ggccaggctg tctccacccg agactcagtg aaattgaact
      241 cgctgtgaag atgcagtgta cccgcggcaa gacggaaaga ccccgtgaac ctttactata
      301 gcttgacact gaacattgag ccttgatgtg taggataggt gggaggcttt gaagtg
//
LOCUS       DAZPLI010000478          349 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZPLI010000478 DAZPLI010000000
VERSION     DAZPLI010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.481"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..>349)
                     /locus_tag="ACHUU3_004669"
     CDS             complement(<1..>349)
                     /locus_tag="ACHUU3_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084496.1"
                     /translation="KFDNFMRQHRFSKVNFDTQQMIHFINRFNMNKGLIDKNNNFFIQ
                     LIDQALRSTDDMIKANAWYLYKEWIRSDDVSPIFIETEEKLRTFNTNKLTRNDNIFIL
                     FSSVDDGPVMVVSS"
BASE COUNT          113 a           60 c           56 g          120 t
ORIGIN      
        1 ggagcttacc accataacag gcccatcatc gacagaggag aacaggataa atatattatc
       61 gtttcgtgtt aatttattag tgttaaaggt tcttaacttt tcttcagttt ctataaatat
      121 aggtgaaaca tcatcactac gaatccactc tttataaaga taccaggcat ttgctttgat
      181 catatcatcc gttgatcgta aggcctgatc gataagttgg ataaaaaaat tgttattttt
      241 atcaatcagc cctttattca tattaaatct gttaataaaa tggatcatct gttgggtatc
      301 gaaattcacc ttactgaatc gatgttgacg cataaaatta tcgaacttg
//
LOCUS       DAZPLI010000479          349 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZPLI010000479 DAZPLI010000000
VERSION     DAZPLI010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.482"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(47..296)
                     /gene="ymgC"
                     /locus_tag="ACHUU3_004670"
                     /pseudo
     CDS             complement(47..296)
                     /gene="ymgC"
                     /locus_tag="ACHUU3_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
BASE COUNT          117 a           64 c           61 g          107 t
ORIGIN      
        1 ttaagtgata ctggttatct ggagattcag ggggccagtc taagttctaa gagagcacgg
       61 attccctgtc attaataatg atatccgtta gtgtagtctt atgcatttca atgtactgca
      121 atatttgcag atagagatca gaaagaatca cactggtttc atcttttaaa cgcaagcgta
      181 aagcctgatc aataatatga ctatcaacat acccatgcgt catgaacacc tccctctcga
      241 gatttttaaa caaagcacat tgaaaaaata aatctctctg ggattgaatt attcatgtca
      301 gcctcgtaga aatattaacc taataaaatt atgctgtgta tagtctaaa
//
LOCUS       DAZPLI010000480          331 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZPLI010000480 DAZPLI010000000
VERSION     DAZPLI010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.483"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            84..263
                     /locus_tag="ACHUU3_004671"
                     /pseudo
     CDS             84..263
                     /locus_tag="ACHUU3_004671"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            211..>331
                     /locus_tag="ACHUU3_004672"
     CDS             211..>331
                     /locus_tag="ACHUU3_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFR2084497.1"
                     /translation="MVVGCSSKENALCHPQPKPPAPPAWAMMPPSNSLQLLDET"
BASE COUNT           92 a           88 c           86 g           65 t
ORIGIN      
        1 gcctgtcatg ggctgttaat cattaccgtg ataacgccat tacctacaaa gagcagcgcg
       61 acaaaaacgc aagagaactg aagctggcga acgcaaccat tactgacatg cagcagcgcc
      121 agcgtgatgc tgatgcactc gatgctaaat acacgaagga gttagctgat gcgaaagctg
      181 aaaatgatgc tcttcggcgc aagcttgata atggtggtcg ggtgctcgtc aaaggaaaat
      241 gccctgtgcc atcctcagcc gaaacctcca gcgcctccgg catgggcaat gatgccaccg
      301 tcgaactctc tccagttgct ggacgaaacg t
//
LOCUS       DAZPLI010000481          330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZPLI010000481 DAZPLI010000000
VERSION     DAZPLI010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.484"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            1..330
                     /locus_tag="ACHUU3_004673"
                     /pseudo
     CDS             1..330
                     /locus_tag="ACHUU3_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           85 a           82 c           98 g           65 t
ORIGIN      
        1 tatgcagcga actgcatatt gccccgtcaa cgtattacca ctgtcagcaa cagcgacatc
       61 atccggataa acgcagtgcc cgtgcgcagc gcgatgactg gctgaagaaa gagatacagc
      121 gcgtatacga tgaaaatcac aaggtatacg gtgtaaagtc tggcgtcagt tgttacggga
      181 aggtatcaga gtggccagat gcactgtggc acgtctcatg gcggttatgg gacttgccgg
      241 tgttctccgg ggtaaaaagg tccgtacgac catcagccgg aaagccgttg ccgcaggcga
      301 ccgcgtaaac cgtcagttcg tggcagaacg
//
LOCUS       DAZPLI010000482          322 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZPLI010000482 DAZPLI010000000
VERSION     DAZPLI010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.485"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            184..>322
                     /locus_tag="ACHUU3_004674"
     CDS             184..>322
                     /locus_tag="ACHUU3_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HFR2084498.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RS"
BASE COUNT           94 a           60 c           59 g          109 t
ORIGIN      
        1 acttgattta agatttgtaa tggctagatt gaaatcagat gtaatccatt agttttatat
       61 tttacccatt tagggctgat ttattactac acacagcagt gcaacatctg tcagtacttc
      121 tggtgcttct attttagagg cagctgtcag gtgtgcgatc aataaaaaaa gcggggtttc
      181 atcatgttta atgaagtcca tagtattcat ggtcatacat tattgttgat cactaaacct
      241 tctttgcagg cgacggctct cttgcagcac cttaaacaat cgctggcaat tacaggaaaa
      301 ttacataata ttcaacgttc tc
//
LOCUS       DAZPLI010000483          320 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZPLI010000483 DAZPLI010000000
VERSION     DAZPLI010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.486"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..242)
                     /locus_tag="ACHUU3_004675"
     CDS             complement(<1..242)
                     /locus_tag="ACHUU3_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR2084499.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT           95 a           62 c           69 g           94 t
ORIGIN      
        1 ttaatggata aatccatcaa tttgtggaca ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcgt cattgataat ctggaatgcg
      121 acatcgtcgc gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      181 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      241 attttaaact ccttaaaatg attggatcgc attaaaaaag taggatttat cgataaagta
      301 agcaagtgca aaggactcgt
//
LOCUS       DAZPLI010000484          308 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZPLI010000484 DAZPLI010000000
VERSION     DAZPLI010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.487"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>308
                     /locus_tag="ACHUU3_004676"
     CDS             <1..>308
                     /locus_tag="ACHUU3_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR2084500.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     P"
BASE COUNT           67 a           82 c           92 g           67 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggaccc
//
LOCUS       DAZPLI010000485          295 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZPLI010000485 DAZPLI010000000
VERSION     DAZPLI010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.488"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
BASE COUNT           79 a           66 c           44 g          106 t
ORIGIN      
        1 caaaaagtga acttctgatg atctttccct ggcctgccac catctcttca caagcttatg
       61 cttattacat gttacatgca aaacgtcccg ttttgcatct ttgtgcctac ggcacaccgt
      121 gatctttccc tgatgaggga tatattaact attgaacatt agcgcgaatt atttagtgca
      181 aaaaatcagc atcacgtgat gcttttcctg ctattaataa cctcctctat ttaactatat
      241 ttttcattga tgcaattcgt agatttgaag aagttcaact gccacttttt tattt
//
LOCUS       DAZPLI010000486          295 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZPLI010000486 DAZPLI010000000
VERSION     DAZPLI010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.489"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..279
                     /locus_tag="ACHUU3_004677"
     CDS             <1..279
                     /locus_tag="ACHUU3_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFR2084501.1"
                     /translation="LTYYADDGWAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGA
                     LLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVALPTE"
BASE COUNT           70 a           77 c           89 g           59 t
ORIGIN      
        1 ctgacgtact atgcagatga tggctgggct gaggcggaca ataacatcgc tgaaaatgcg
       61 ttgcggatgg tcagtctggg ccgcaaaaac tacctgttct tcggttcgga tcatggagga
      121 gagcggggag cgctgctgta cagcctgatc gggacgtgca aactgaacgg agtggagcca
      181 gaaagctacc tccgctatgt ccttgacgtc atagccgact ggccgataaa ccgggtcggc
      241 gaactgctcc cctggcgcgt agcactgccg actgaataac acattcccgt caata
//
LOCUS       DAZPLI010000487          292 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZPLI010000487 DAZPLI010000000
VERSION     DAZPLI010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.490"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>292
                     /locus_tag="ACHUU3_004678"
     CDS             <1..>292
                     /locus_tag="ACHUU3_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR2084502.1"
                     /translation="RGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNG
                     GDWFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEW"
BASE COUNT           78 a           56 c           80 g           78 t
ORIGIN      
        1 atcgtgggcc gcaaagttta cgttgtctgc agttactgga acaacattgg gtttgtgcgg
       61 taagaggcaa tcatgaacag atggcgatgg atgcgctggc atcccagcag atgtctttgt
      121 ggttgatgaa tggcggcgac tggtttattg cgctggcaga taatcaacag aaacaagcga
      181 aaacggcgct ggaaaaatgt cagcatttgc cctttattct tgaagtacac agtcgtaccg
      241 gcaaacatgt tattgctcat gccgattatc cagatgatgt ttatgaatgg ca
//
LOCUS       DAZPLI010000488          290 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZPLI010000488 DAZPLI010000000
VERSION     DAZPLI010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.491"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..228)
                     /locus_tag="ACHUU3_004679"
     CDS             complement(<1..228)
                     /locus_tag="ACHUU3_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFR2084503.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
BASE COUNT          103 a           44 c           59 g           84 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa
//
LOCUS       DAZPLI010000489          289 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZPLI010000489 DAZPLI010000000
VERSION     DAZPLI010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.492"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            1..192
                     /locus_tag="ACHUU3_004680"
                     /pseudo
     CDS             1..192
                     /locus_tag="ACHUU3_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
BASE COUNT           71 a           58 c           97 g           63 t
ORIGIN      
        1 catatggcgt ggggtgatgc cgataatcag taggtgaaac tgtcgatgcc ggaactggaa
       61 gaactggcgg cagcaatggt gcaggctgtt gttgaacgca atgacgagat ttatcgccgt
      121 cagcgtgaaa tgaaagagga gctgagcagt ctggatgatt tggcttcaat tcgggcgttt
      181 gacgttaagt aatgaataag ccgcaactgg cggaatcaca gaagaccgct ttgcttaccg
      241 aggcggagtc tgtcatccgg ccgccggggt gtgcggtcag gctgaacag
//
LOCUS       DAZPLI010000490          287 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZPLI010000490 DAZPLI010000000
VERSION     DAZPLI010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.493"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..>287
                     /locus_tag="ACHUU3_004681"
     CDS             <1..>287
                     /locus_tag="ACHUU3_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR2084504.1"
                     /translation="RLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVCYRGQVHH
                     FAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGAL"
BASE COUNT           72 a           55 c           89 g           71 t
ORIGIN      
        1 gcgacttatc agtggaaaaa cacagaacga tcaggatata tgcgccctgg caaaagagta
       61 tgagatcgcc atgctgttgg tgactaaagg tgcagaaggg gtggtggtct gttatcgagg
      121 acaagttcac cattttgctg gaatgtctgt gaattgtgtc gatagcacgg gggcgggaga
      181 tgcgttcgtt gccgggttac tcacaggtct gtcctctacg ggattatcta cagatgagag
      241 agaaatgcga cgaattatcg atctcgctca acgttgcgga gcgcttg
//
LOCUS       DAZPLI010000491          278 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZPLI010000491 DAZPLI010000000
VERSION     DAZPLI010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.494"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..152
                     /locus_tag="ACHUU3_004682"
     CDS             <1..152
                     /locus_tag="ACHUU3_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFR2084505.1"
                     /translation="KGNNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQ
                     KIGGL"
BASE COUNT          101 a           32 c           76 g           69 t
ORIGIN      
        1 cgaaaggtaa taataaatgt gaaatttatt ttgatgcttt tggtgaatgt ggaagtgtga
       61 atggcattaa cgtgtgtttt gaaaaaaatg aaatgatagg ttggattaaa aaagaaatcc
      121 ctttgctgtc gcaaaaaata ggtgggcttt aaatggtagg gcaatattca aagacagtat
      181 aggtaactat ttgtatggcc atgtaaggta aaagaaaagg aataaaccat gagcggaaaa
      241 ccggcggcgc ggcagggcga catgacccgc agggaaag
//
LOCUS       DAZPLI010000492          278 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZPLI010000492 DAZPLI010000000
VERSION     DAZPLI010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.denovo.495"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(<1..71)
                     /locus_tag="ACHUU3_004683"
     CDS             complement(<1..71)
                     /locus_tag="ACHUU3_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084506.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLK"
BASE COUNT           87 a           63 c           39 g           89 t
ORIGIN      
        1 tcttttaacc ctgcttgcat aacaggtatt ttactgataa caattccatt tttgcagcat
       61 cctggcaaca tatttcctcc acatccactg tctaaaaatc atgatggttt attcaggata
      121 ttcattcctt aaatgtttcc agcaaattaa tagattaagc gaataaatta tccttagcat
      181 tcactttcat atcagcatac tcctgctgca caacaactcc actgcatgtg tcagaaggtt
      241 agcatcaaat gataacctgg tgaaatagcc tggagaca
//
LOCUS       DAZPLI010000493        21432 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.guided.063, whole genome shotgun sequence.
ACCESSION   DAZPLI010000493 DAZPLI010000000
VERSION     DAZPLI010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.guided.063"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            complement(34..204)
                     /locus_tag="ACHUU3_004684"
                     /pseudo
     CDS             complement(34..204)
                     /locus_tag="ACHUU3_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(423..1400)
                     /gene="epmA"
                     /locus_tag="ACHUU3_004685"
     CDS             complement(423..1400)
                     /gene="epmA"
                     /locus_tag="ACHUU3_004685"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR2084507.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1725..3533
                     /gene="frdA"
                     /locus_tag="ACHUU3_004686"
     CDS             1725..3533
                     /gene="frdA"
                     /locus_tag="ACHUU3_004686"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR2084508.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3526..4260
                     /gene="frdB"
                     /locus_tag="ACHUU3_004687"
     CDS             3526..4260
                     /gene="frdB"
                     /locus_tag="ACHUU3_004687"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR2084509.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4271..4666
                     /gene="frdC"
                     /locus_tag="ACHUU3_004688"
     CDS             4271..4666
                     /gene="frdC"
                     /locus_tag="ACHUU3_004688"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR2084510.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4677..5036
                     /gene="frdD"
                     /locus_tag="ACHUU3_004689"
     CDS             4677..5036
                     /gene="frdD"
                     /locus_tag="ACHUU3_004689"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR2084511.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5099..6232
                     /locus_tag="ACHUU3_004690"
     CDS             5099..6232
                     /locus_tag="ACHUU3_004690"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR2084512.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6321..6854
                     /gene="blc"
                     /locus_tag="ACHUU3_004691"
     CDS             6321..6854
                     /gene="blc"
                     /locus_tag="ACHUU3_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR2084513.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="ACHUU3_004692"
     CDS             complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="ACHUU3_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR2084514.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7343..7489)
                     /gene="ecnB"
                     /locus_tag="ACHUU3_004693"
     CDS             complement(7343..7489)
                     /gene="ecnB"
                     /locus_tag="ACHUU3_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR2084515.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7600..7725)
                     /gene="ecnA"
                     /locus_tag="ACHUU3_004694"
     CDS             complement(7600..7725)
                     /gene="ecnA"
                     /locus_tag="ACHUU3_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR2084516.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7777..8343)
                     /gene="efp"
                     /locus_tag="ACHUU3_004695"
     CDS             complement(7777..8343)
                     /gene="efp"
                     /locus_tag="ACHUU3_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR2084517.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8385..9413
                     /gene="epmB"
                     /locus_tag="ACHUU3_004696"
     CDS             8385..9413
                     /gene="epmB"
                     /locus_tag="ACHUU3_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR2084518.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9803..10672
                     /locus_tag="ACHUU3_004697"
     CDS             9803..10672
                     /locus_tag="ACHUU3_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR2084519.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLNAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGTVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(10875..11228)
                     /locus_tag="ACHUU3_004698"
     CDS             complement(10875..11228)
                     /locus_tag="ACHUU3_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR2084520.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAVMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11366..13012)
                     /gene="groL"
                     /locus_tag="ACHUU3_004699"
     CDS             complement(11366..13012)
                     /gene="groL"
                     /locus_tag="ACHUU3_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR2084521.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13056..13349)
                     /locus_tag="ACHUU3_004700"
     CDS             complement(13056..13349)
                     /locus_tag="ACHUU3_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR2084522.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13625..14881
                     /gene="yjeH"
                     /locus_tag="ACHUU3_004701"
     CDS             13625..14881
                     /gene="yjeH"
                     /locus_tag="ACHUU3_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR2084523.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(14903..15373)
                     /locus_tag="ACHUU3_004702"
     CDS             complement(14903..15373)
                     /locus_tag="ACHUU3_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR2084524.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            15710..17146
                     /gene="aspA"
                     /locus_tag="ACHUU3_004703"
     CDS             15710..17146
                     /gene="aspA"
                     /locus_tag="ACHUU3_004703"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR2084525.1"
                     /translation="MSNNIRIEEDLLGTKEVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17264..18565
                     /gene="dcuA"
                     /locus_tag="ACHUU3_004704"
     CDS             17264..18565
                     /gene="dcuA"
                     /locus_tag="ACHUU3_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR2084526.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            18681..19019
                     /gene="cutA"
                     /locus_tag="ACHUU3_004705"
     CDS             18681..19019
                     /gene="cutA"
                     /locus_tag="ACHUU3_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR2084527.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            18995..20692
                     /gene="dsbD"
                     /locus_tag="ACHUU3_004706"
     CDS             18995..20692
                     /gene="dsbD"
                     /locus_tag="ACHUU3_004706"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR2084528.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            20729..21304
                     /locus_tag="ACHUU3_004707"
     CDS             20729..21304
                     /locus_tag="ACHUU3_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR2084529.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         5285 a         5521 c         5363 g         5263 t
ORIGIN      
        1 ccatcataca ctaaatcagt aagttggcag catcaccatt aaggcgaatg gaataaagaa
       61 taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga
      121 tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact
      181 catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt
      241 gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga
      301 tacccggatg atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac
      361 atatgctaca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag
      421 aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc
      481 aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat accgactttc
      541 aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta
      601 cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca
      661 ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg
      721 gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt
      781 tctttgccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg
      841 tcttcttcgg tatcagcaac gttactcaaa tccagtttcg ctgcgacttc ccgcagttgc
      901 gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa
      961 aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc
     1021 aaccggtaca tatcatagtg cggtcgatac cactcaagca tagtgaactc agggttgtga
     1081 taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaaccg
     1141 gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc
     1201 tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc
     1261 gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga
     1321 cggatctccg ccataatcgc cgcacgtttt aataagttag gaatggatgc gctcggctgc
     1381 caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact
     1441 cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa
     1501 aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc
     1561 tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg
     1621 ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc
     1681 ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag
     1741 ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc
     1801 aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata
     1861 ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc
     1921 actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt
     1981 tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtgggggtgc ccatggagcc
     2041 gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct
     2101 ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc
     2161 aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg
     2221 gtcatgttcg cggcctggta gcaatgaaca tgatggaagg cacgctggtg cagatccgtg
     2281 ctaacgcggt cgttatggct actggcggtg cgggtcgcgt ttatcgttac aacaccaacg
     2341 gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg
     2401 acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg
     2461 aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag
     2521 attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg
     2581 gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatct
     2641 ccacgccgcg tggcgatgtg gtttatctcg acttgcgtca cctcggcgag aaaaaactgc
     2701 atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta
     2761 aagaaccgat tccggtacgt ccgaccgcac actacaccat gggcggtatc gaaaccgatc
     2821 agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc
     2881 tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc
     2941 tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa
     3001 ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg
     3061 gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta
     3121 tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac
     3181 gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca
     3241 ccattgaact gggccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
     3301 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
     3361 tcaacttcct caaacacacc ctcgccttcc gtgatgctga tggcacgact cgcctagagt
     3421 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
     3481 atgcagccga taaggcggaa gcagccaata agaaggagaa ggcgaatggc tgagatgaaa
     3541 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
     3601 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa
     3661 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
     3721 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
     3781 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
     3841 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
     3901 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
     3961 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
     4021 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
     4081 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggcgt atggagctgt
     4141 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
     4201 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
     4261 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
     4321 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
     4381 gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg
     4441 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
     4501 ggcggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
     4561 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
     4621 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
     4681 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
     4741 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
     4801 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
     4861 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
     4921 gtatgcacca cgcgatgcac gatctgaaaa ttcacgtgcc tgcgggcaaa tgggttttct
     4981 acggtctggc tgctatcctg acagttgtca cgctgattgg tatcgttaca atctaacgta
     5041 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
     5101 gttcaaaacg acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc
     5161 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
     5221 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
     5281 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
     5341 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
     5401 aagcgatccc gcaacaaaat actggcctga acttactgct aaacagtgga atgggatcac
     5461 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt
     5521 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg
     5581 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
     5641 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
     5701 ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga
     5761 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
     5821 gaccattgaa gatatggccc gctgggtgca aagcaattta accccccttg atatcaatga
     5881 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     5941 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     6001 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     6061 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     6121 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     6181 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     6241 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     6301 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     6361 ttctggtcgt tgcctgcagt tctcctacgc cgccgcgtgg cgtgacagta gtgaataatt
     6421 tcgacgctaa acgctatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
     6481 aacgtggact ggaaaaagtc accgcaacat acagtctgcg tgatgacggc ggcctgaatg
     6541 tcatcaataa aggctataac cctgacagag aaatgtggca gcagagtgaa gggaaagcgt
     6601 actttaccgg cgcaccaaca cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
     6661 gcggttataa cgtcattgca cttgatcggg aataccgcca tgcgctggtt tgcggcccgg
     6721 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
     6781 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
     6841 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
     6901 gactcgccag gcgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
     6961 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt aacgatttca
     7021 tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacagtaata acacttggcg
     7081 tcaaacgact aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac
     7141 cagcaataac taagataatc caggacatat caggctccgg agaattgggg ccgtccccgg
     7201 tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag
     7261 gtggcagggg aatttcaact attacctttg tgactatcgc aaaaaaacaa acggcacgac
     7321 ataactgtcg taccgttatt gcttattgct gcgctttcgt tgctgcacca gaaatcgcgt
     7381 taccgccatc agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca gttaatactg
     7441 ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata acgtcttcct
     7501 tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac atcgccaaaa
     7561 atgatggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag cgcgggagat
     7621 ggagttgccg agatgtttga tgtcttcgcc gaaaccgcga gcggtgttac agcccgtgag
     7681 cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg acctgaataa
     7741 gtgatggtgc agcctgcagg ccgcaccaca accgcattac ttcacgcgag agacgtattc
     7801 accagagcgg gtatccactt tgatgacttc gccgatttgt acgaacagcg gaactttaac
     7861 cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat cacctttcag
     7921 gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa cggagatcgg
     7981 ctgaccattc cacagagtta cgatacactc tgcctgatcc agcagccatt tagcgttgtc
     8041 gccaattgct tttgcatcag cagacagctg ctcgaaagtt tcgttgttca tgaagtgcca
     8101 gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag cgccttcagc
     8161 ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac gacgcagttt
     8221 aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt caaccgcgta
     8281 aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat agtacgttgc
     8341 cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg catattgtaa
     8401 ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt gtgaccgatc
     8461 ctggtgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg gccggacgca
     8521 gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc atggagaaag
     8581 gcaatccgga cgatcctatt ttgcgtcagg tacttacctc gcaagatgag tttgtcgtcg
     8641 cgtccggatt ctccaccgac ccactggaag aacagcacag cgtagtgcct ggtttgttgc
     8701 ataaatacca caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta aattgccgct
     8761 attgcttccg tcgccacttc ccctatgccg aaaatcaggg caacaagcgt aactggcaga
     8821 ctgcacttga gtatgttgct gcgcatccgg aactggacga gatgattttc tccggcggcg
     8881 atccgctgat ggcgaaagat cacgagctgg actggttgct cacacaactg gaagccatcc
     8941 cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgatcccg gcacgtatca
     9001 ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg gtgaatcaca
     9061 tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc gatggctaaa ttgcgccgtg
     9121 tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg tgtgaacgat aacgcacaaa
     9181 cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt gatgccgtat tacctgcatg
     9241 tgctagataa agtacagggc gcggcgcatt ttatggtgag tgatgacgaa gcacggcaga
     9301 ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt gccgaaactg gcgcgagaaa
     9361 ttggcggcga acccagcaaa acgccgctgg atctccagct acgccagcag taactgatta
     9421 aacagggaag ttaaacatta gcctgatgcg ctacgcttat caggcctaca ttgtgcattg
     9481 aaattgaatg gatttgcaat gaactgtagg ccggataagg cgtttacgcc gcatccggca
     9541 agagcaacaa acgcaagtac agcaacctgt cattaaactg ggaaatttaa tctttgcccg
     9601 ttgcataacc tttaattatt catatacatt gctgttgtta ctctttatat agccaacacc
     9661 aataattaac ctttcttttc tgtttatata gagtgagtac ggctatttat gttattttaa
     9721 caactattac taccaagccc atttatctgg cttataaatt aaaattaatt atttaattga
     9781 gaaatggtta gggagaacct acatggccat aagcatcaag ggtgttaata ccggtgttat
     9841 tcgcaagagt aataatttca ttgcactggc gctgaaaatt aaagaacctc gcaataaaga
     9901 atcgttgttc tttatgtctg tcatggaact gcgcgacctg ctgattgcgc tggaaagtcg
     9961 tctgcatcaa aagcataaac tgaatgctgc tgcacgcctt cagtatgagc aagctcgcga
    10021 taaagtcatt aagaaaatgg cagaaaacat cccagaaatt ctggttgatg agcttaaaaa
    10081 tgccgatatt aatcgtcggg ttaacacgct ggaacttacc gataatcaag gcgaaaatct
    10141 taccttcgta ttaacgctac atgacggtag caaatgtgag ttagtcgtca atgagttgca
    10201 gattgaaatg ctggcgcgag caatcattca tgcaattaat aatgcggaaa tgcgtgaact
    10261 ggcattacgt attacctcac tgttagattt cctgccgtta tacgacgttg actgccagga
    10321 caatggcaat ctggaatatg acacctattc ccaaccggaa tggaaacata acctgttcga
    10381 ccattatctg gcggtgcttt accgctttaa agatgaaagt ggcaaagaac aatttagcgg
    10441 cacagtggtg aaaacgcgtg aagcaacgcc gggtaaagaa atagaggcga tcactcgccg
    10501 aatgcttgat ttcagcccac gactgaaaaa actcgcgggt gtgccatgtc aggtctatgt
    10561 tcgcaccgta gcggcgaata acgcccaacc gctaacccag gatcaatgct tacgcgcgct
    10621 gcatcactta cgtgttcaat ccaccagcaa aactgcacca caggcgaaat aatctcatct
    10681 ttgctaatgc gaggctggag ccatccagcc tgaaatgact gataacgtga tcgttggtca
    10741 tgccggatgc gacgtaaacg ccttatccgg cctacaaatt ctcaagcatt caatcagttg
    10801 caatgcctgc tgtaggcctg ataagcgaag cgcatcaggc aataatacgt ttgtcaccca
    10861 gcctctggat cacatcagtt cgggcactta taaacctgac cgataatctg gctatccgtc
    10921 gggacaaaac tggacaacat tccctgcgac gggctgctga tgccataaat cacgttaccg
    10981 cccattacag ccgcctggtt gcgcagatcg tttgctgcgc cgcgcataga accgccctct
    11041 tctccgtgtt gcccggaaag ccagttgctt tgcttacctg tcgcagtacc aatcagctgg
    11101 cactctgcgc ctggctgctc gtccacaatg cgtacactct gaccggcagc actcaattcg
    11161 ttgctggagc tacaacccgc catcagtagc gcggcaccga caatccctgc taagtatttt
    11221 acgtgcatgt tattccccat aatcaatgag ctggacgcca ctcgcgtcgt ccgtgtctga
    11281 atcttatact agaaagatga ccaaaagaaa aacccccaga catttctgcc cgggggtttg
    11341 tttatttctg cgaggtgcag ggcaattaca tcatgccgcc catgccaccc atgccgccca
    11401 taccgccagc agcgcctaag tcagctgcat catttttcgg caggtcggta accatgcact
    11461 cggtggtgat catcaggcca gccacagaag ccgcgtactg cagagcagaa cgggttactt
    11521 tggttgggtc caggataccc atgtcgatca tgttgccgta ttcttcggtt gctgcgttgt
    11581 aaccgtagtt gccgtcgccg cctttaacgg tgttagcaac aacagacggt tcttcgccgc
    11641 agttcaggac gatctgacgc agcggagctt ccattgcacg cagtgcaact ttgataccca
    11701 cgttctggtc ttcgttctga ccacgcaggt cagccagttt agacgctacg cggatcagcg
    11761 caacaccacc accagcaacc acgccttctt ctaccgcagc acgggtcgcg tgcagggcgt
    11821 cttcaacgcg tgcttttttc tctttcattt caacttcggt agcagcacct actttgataa
    11881 ctgcaacgcc gcctgccagt ttcgctacgc gttcctgcag tttttcacgg tcgtagtcag
    11941 aagttgcttc ttcaatctgc tgacggatct gagcaacacg gccctggatt gcagcttctt
    12001 cacccacgcc atcgatgata gtggtggtgt ctttgttgat cacaacacgt ttagcctgac
    12061 ccaggtcttc cagggttgct ttttccagtt ccataccgat ctcttcagag atcacggtac
    12121 cgccagtcag ggttgcgata tcctgcagca tagctttacg acgatcgccg aagcccggag
    12181 ctttaactgc agcaactttc acgatgccac gcatggtgtt aacaaccaga gttgccagcg
    12241 cttcgccttc tacatcttca gcgatgatca gcagcggttt gcctgctttg gcaacggctt
    12301 ccagaaccgg cagcatttcg cggatgttgg agattttctt gtcagccagc aggatgaacg
    12361 ggctttccag ttctactgcg ccagtttccg gcttgttgat gaagtaagga gacaggtagc
    12421 cacggtcgaa ctgcatacct tcaaccacgt ccagttcgtc ctgcagaccg gtaccgtctt
    12481 caacggtgat aacgccttct ttaccgactt tgtccatcgc ttcagcgatc agtttaccta
    12541 cggtttcgtc ggagttagcg gagatggtac caacctgagc aatcgcttta gagtcagagc
    12601 acggtacgga cagcgctttc agttcttcaa ctgcagcggt aacggctttg tcgataccac
    12661 gtttcaggtc catcgggttc atgcccgcag caacagcttt cagaccctca gtgatgatag
    12721 cctgagccag tacagttgca gtggtggtac cgtcgcctgc agcgtcgttc gctttagagg
    12781 caacttcttt caccatctgc gcacccatgt tttcgaactt gtcttccagt tcgatttcac
    12841 gagcaacgga aacaccatct ttggtgatgg tcggtgcacc gaaagattta tccagaacta
    12901 cgttacggcc tttcggaccg agggtaactt tcactgcatc tgccagtacg tttacgccgc
    12961 gcagcatttt cacacgagcg tcgttaccga attttacgtc tttagctgcc attatcttta
    13021 ttccttaaat tcgtatgttc agtgtcgtgc gaggattacg cttcaacaat tgccagaatg
    13081 tcgctttcgg acatgatcaa cacttcttca ttgtcgatct tctcagattt cacaccgtag
    13141 ccatcgttga aaataacgat gtcgccaact ttcacatcca gcggcttcac ttcgccattt
    13201 tcaaggatac ggccattgcc gacagccagc acttcgccgc gggtggattt agccgctgca
    13261 gagccggtca gaacgatgcc gccagcagat ttagtttcaa cttctttacg cttgacgatc
    13321 acgcgatcat gcaatggacg aatattcatt gataactctc ctttgagaaa gtccgtatct
    13381 gttatgggtg acgccggagc ttacgtggtt tcccggctgg tgaccagaga aatggggatg
    13441 aggattcgcc ccttcaaggg ggaaaaagaa aaaaaattct gatcagcaca aaatcgggtg
    13501 aaaaccctga ttcacctcac atttcatcgc aatttcttca tcgccgtcat aagcgaatct
    13561 gattgtgcta ccatcgaaaa tctacgcagt tgcccaaaat ttgggcgcaa tcggatatca
    13621 acccatgagt ggactcaaac aagaactggg gctggcccag ggcattggcc tgctatcgac
    13681 gtcattatta ggcaccggcg tgtttgccgt tcctgcgtta gctgcacagg tagcaggcaa
    13741 taacagcctg tgggcgtggc ccgttttgat tatcttagtg ttcccgattg cgattgtgtt
    13801 tgcgattctg ggtcgccact atcccagcgc aggcggtgtc gcgcacttcg tcggtatggc
    13861 gtttggttcg cggcttgagc gagtcaccgg ctggttgttt ttatcggtca ttcccgtggg
    13921 tttgcctgcc gcgctacaaa ttgccgccgg gttcggccag gcaatgtttg gctggcatag
    13981 ctggcaactg ttgttggcag aactcggtac gctggcgctg gtgtggtata tcggtactcg
    14041 cggtgccagt tccagtgcta atctacaaac cgttattgcc ggacttatcg tcgcgctgat
    14101 tgtcgctatc tggtgggcgg gcgatatcaa acctgcgaat atccccttcc cggcacctgg
    14161 taatatcgaa cttaccgggt tatttgctgc gttatcagtg atgttctggt gttttgtcgg
    14221 tctggaagca tttgcccatc ttgcctcgga atttaaaaat ccagagcgtg attttcctcg
    14281 cgcattgatg attggtctgc tgctggcagg attagtctac tggggctgta cggtagtcgt
    14341 cttacacttc gacgcctatg gtgaacaaat ggccgcggca gcatcgcttc caaaaattgt
    14401 agtgcaactg ttcggtgtag gagcgttatg gattgcctgc gtaattggct atctgacctg
    14461 ctttgccagt ctcaacattt atatacagag cttcgcccgc ctggtctggt cgcaggcgca
    14521 acataatcct gaccactacc tggcacgcct ctcttctcgc catatcccga ataatgccct
    14581 caatgcggtg ctcggctgct gcgtggtgag cactttggtg attcatgctt tagagatcaa
    14641 tctggacgct cttattattt atgccaatgg catctttatt atgatttatc tgttatgcat
    14701 gctggcaggc tgtaaattat tgcaaggacg ttatcgacta ctggcggtgg ttggcgggct
    14761 gttatgcgtt ctgttactgg caatggtcgg ctggaaaagt ctctatgcgc tgattatgct
    14821 ggcggggtta tggctgtttc tgccaaaacg aaaaacgccg gaaaatggca taaccacata
    14881 atccggcgtt tcgacattaa tcctagcgat cgtctttatg atcaaggcgg tcgcggtcat
    14941 catcctttcg ctggtactca ccatcaaaag tattaccgcc accggtcccg gcgctaaaac
    15001 cgccgcctgg catgcgagaa aagcgcaaat gtggcatcaa cttcactgtc agatgctttt
    15061 gcaccggcgg caataaaagc agaagaccga ggaagtcggt gaaaaagccc ggtaataaaa
    15121 gcagcaaacc ggcaataatc agcgaaacac ttttaatcat ctccgccgca gggttttcac
    15181 ccgccgccat tttttgctgc atcagcacga aattcttaaa gccctggtta cgtaccagtg
    15241 acataccgat aactgacgtg aatataacca gcacgagggt cagcaatacc cccaatacat
    15301 gggcaacctg aataaagatt gaaatctcaa tatagacata aaggaaaatg gcaataaaag
    15361 gtaaccagcg caaaggtttc tcctgtaata gcagccggtt aaacccggct acctgaatgg
    15421 gttgcgaatc gcgttcagct tatattgtgg tcattagcaa aatttcaaga tgtttgcgca
    15481 actatttttg gtagtaatcc caaagcggtg atctatttca caaattaata attaaggggt
    15541 aaaaaccgac acttaaagtg atccagatta cggtagaaat cctcaagcag catatgatct
    15601 cgggtattcg gtcgatgcag gggataatcg tcggtcgaaa aacattcgaa accacatata
    15661 ttctgtgtgt ttaaagcaaa tcattggcag cttgaaaaag aaggttcaca tgtcaaacaa
    15721 cattcgtatc gaagaagatc tgttgggtac caaggaagtt ccagctgatg cctactatgg
    15781 tgttcacact ctgagagcga ttgaaaactt ctatatcagc aacaacaaaa tcagtgatat
    15841 tcctgaattt gttcgcggta tggtaatggt taaaaaagcc gcagctatgg caaacaaaga
    15901 gctgcaaacc attcctaaaa gtgtagcgaa tgccatcatt gccgcatgtg atgaagtcct
    15961 gaacaacgga aaatgcatgg atcagttccc ggtagacgtc taccagggcg gcgcaggtac
    16021 ttccgtaaac atgaacacca acgaagtgct ggccaatatc ggtctggaac tgatgggtca
    16081 ccagaaaggt gaatatcagt acctgaaccc gaacgaccat gttaacaaat gtcagtccac
    16141 taacgacgcc tacccgaccg gtttccgtat cgcagtttac tcttccctga ttaagctggt
    16201 agatgcgatt aaccaactgc gtgaaggctt tgaacgtaaa gctgtcgaat tccaggacat
    16261 cctgaaaatg ggtcgtaccc agctgcagga cgcagtaccg atgaccctcg gtcaggaatt
    16321 ccgcgctttc agcatcctgc tgaaagaaga agtgaaaaac atccaacgta ccgctgaact
    16381 gctgctggaa gttaaccttg gcgcaacagc aatcggtact ggtctgaaca cgccgaaaga
    16441 gtactctccg ctggcagtga aaaaactggc tgaagtcact ggcttcccat gcgtaccggc
    16501 tgaagacctg atcgaagcga cctctgactg cggcgcttat gttatggttc acggcgcgct
    16561 gaaacgcctg gctgtgaaga tgtccaaaat ctgtaacgac ctgcgcttgc tctcttctgg
    16621 tccacgtgcc ggcctgaacg agatcaacct gccggaactg caggcgggct cttccatcat
    16681 gccagctaaa gtaaacccgg ttgttccgga agtggttaac caggtatgct tcaaagtcat
    16741 cggtaacgac accactgtta ccatggcagc agaagcaggt cagctgcagt tgaacgttat
    16801 ggagccggtc attggccagg ctatgttcga atccgttcac attctgacca acgcttgcta
    16861 caacctgctg gaaaaatgca ttaacggcat cactgctaac aaagaagtgt gcgaaggtta
    16921 cgtttacaac tctatcggta tcgttactta cctgaacccg ttcatcggtc accacaacgg
    16981 tgacatcgtg ggtaaaatct gtgccgaaac cggtaagagt gtacgtgaag tcgttctgga
    17041 acgcggtctg ttgactgaag cggaacttga cgatattttc tccgtacaga atctgatgca
    17101 cccggcttac aaagcaaaac gctatactga tgaaagcgaa cagtaatcgt acagggtagt
    17161 acaaataaag aaggcacgtc agatgacgtg ccttttttct tgtgagcagt aacttaaaaa
    17221 taacaatcta atatcaactt gttaaaaaac aaggaaggct aatatgctag ttgtagaact
    17281 catcatagtt ttgctggcga tcttcttggg cgccagattg gggggaatag gtattggttt
    17341 tgcaggcgga ttgggggtgc tggttcttgc cgctattggc gttaaacccg gtaacatccc
    17401 gttcgatgtc atttccatta tcatggcggt tatcgccgct atttctgcca tgcaggttgc
    17461 tggcggtctg gactatctgg ttcatcagac agaaaagctg ctgcgccgta acccgaaata
    17521 catcacaatc ctcgcaccga tcgtgaccta tttcctgaca atctttgctg gtactggcaa
    17581 catctctctg gcgacactgc cagttatcgc tgaagttgcg aaggaacaag gcgttaaacc
    17641 ttgccgtccg ctgtctactg cagtggtatc cgcgcagatt gcgatcaccg catcgccaat
    17701 ctcagcggca gtggtttaca tgtcttccgt gatggaaggt catggcatca gctacctcca
    17761 tctgctctcc gtggtcatcc cgtccaccct gctggcggtt ctggtgatgt ccttcctggt
    17821 cactatgctg ttcaactcca aactctctga cgatccgatt tatcgcaagc gtctggaaga
    17881 gggcctggtt gaactgcgcg gtgaaaagca gattgaaatc aaatccggtg caaaaacgtc
    17941 cgtctggctg ttcctgctgg gcgtagttgg cgtggttatc tatgcaatca tcaacagccc
    18001 aagcatgggt ctggttgaaa aaccgctgat gaacaccacc aacgcaatcc tgatcatcat
    18061 gctcagcgtt gcaactctga ccaccgttat ctgtaaagtc gataccgaca acatcctcaa
    18121 ctccagcacc ttcaaagcag gtatgagcgc ctgtatttgt atcctgggtg ttgcgtggct
    18181 gggcgatact ttcgtttcca acaacatcga ctggatcaaa gataccgctg gtgaagtgat
    18241 tcagggtcat ccgtggctgc tggccgtcat cttcttcttt gcttctgctc tgctgtactc
    18301 tcaggctgca accgcaaaag cactgatgcc gatggctctg gcactgaacg tttctccgct
    18361 gaccgctgtt gcttctttcg ctgcggtgtc tggtctgttc attctgccga cctatccgac
    18421 gctggttgct gcggtacaga tggatgacac gggtacaacc cgtatcggta aattcgtctt
    18481 caaccatccg ttcttcatcc cgggtactct gggtgttgcc ctggccgttt gcttcggctt
    18541 cgtgctgggt agcttcatgc tgtaatgacc aatcgcgggg cgttcacgcc ccgctttctt
    18601 tcccgccgac taacatcctt cccccgtccg ttgtatagtg acctctctct tgcggttcca
    18661 tctgttcttg cgaggtgttt atgcttgatg aaaaaagttc gaataccgcg tctgtcgtgg
    18721 tgctatgtac cgcaccggat gaagcgacag cccaggattt agccgccaaa gtgctggcgg
    18781 aaaaactggc ggcctgcgcg accttgatcc ccggcgctac ctctctctat tactgggaag
    18841 gtaagctgga gcaagaatac gaagtgcaga tgattttaaa aactaccgta tctcaccagc
    18901 aggcactgct ggaatgcctg aagtctcatc atccatatca aaccccggaa cttctggttt
    18961 tacctgttac acacggagac acagattacc tctcatggct caacgcatct ttacgctgat
    19021 cctgctactt tgcagcactt ccgtttttgc cggattattc gacgcgccgg gacgttcaca
    19081 ttttgtcccc gcggatcaag cctttgcttt tgattttcag caaaaccaac atgacctgaa
    19141 tctgacctgg cagatcaaag acggttacta cctctaccgt aaacagatcc gcattacgcc
    19201 ggaacacgcg aaaattgccg acgtgcagct gccgcaaggc gtctggcatg aagatgagtt
    19261 ttacggcaaa agcgagattt accgcgatcg gctgacgctt cccgtaacca tcaaccaggc
    19321 gagtgcggga gcgacgttaa ctgtcaccta ccagggctgt gctgatgccg gtttctgtta
    19381 tccgccagaa accaaaaccg ttccgttaag cgaagtggtc gccaacaacg cagcgtcaca
    19441 gcctgtgtct gttccgcagc aagagcagca caccgcgcaa ttgccctttt ccgcgctctg
    19501 ggcgttgttg atcggtattg gtatcgcctt tacgccatgc gtgctgccaa tgtacccact
    19561 gatttctggc atcgtgctgg gtggtaaaca gcggctctcc actgccagag cattgttgct
    19621 gacctttatt tatgtgcagg ggatggcact gacttacacg gcgctgggtc tggtagttgc
    19681 cgccgcaggg ttacagttcc aggcggcgct acagcaccca tacgtgctca ttggcctcgc
    19741 catcgtcttt accttgctgg cgatgtcaat gtttggcttg tttactctac aactcccctc
    19801 ttcgctgcaa acacatctca cattgatgag caatcgccaa cagggcggct cacctggcgg
    19861 tgtgtttgtt atgggggcga ttgccggact gatctgttca ccatgcacca ccgcaccgct
    19921 tagcgcgatt ctgctgtata tcgcccaaag cgggaacatg tggctgggcg gcggcatgct
    19981 ttatctctat gcgttgggca tgggcctgcc gctgatgcta attaccgtct ttggtaaccg
    20041 cttgctgccg aaaagcggcc cgtggatgga acaagtcaaa accgcgtttg gttttgtgat
    20101 cctcgcactg ccgatcttcc tgctggagcg agtgattggt gatatatggg gattacgctt
    20161 gtggtcggcg ctgggtgtcg cattctttgg ctgggccttt atcaccagcc tacaggccaa
    20221 acgcggctgg atgcgcgtgg tgcaaataat cctgctggca gcggcattgg ttagcgtgcg
    20281 cccacttcag gattgggcat ttggtgcgac gcataccgcg caaactcaga cgcatctcaa
    20341 ctttacacaa atcaaaacgg tagatgagtt aaatcaggcg ctcgttgaag ccaaaggcaa
    20401 accggtgatg ttagatcttt atgccgactg gtgcgtcgcc tgtaaaaagt ttgagaaata
    20461 caccttcagc gacccgcagg tgcaaaaagc gttagcagac acggtcttac ttcaggccaa
    20521 cgtcactgct aacgacgcac aagatgtggc gctgttaaag catcttaatg tccttggact
    20581 accgacaatt ctcttttttg acggacaagg ccaggagcat ccacaagcac gcgtcacggg
    20641 ctttatggat gctgaaacct tcagcgcaca tttgcgcgat cgccaaccgt gaacgacact
    20701 tgcagtggga aagacggagg aaaatagcgt gcaacgtgaa gatgtactgg gagaagccct
    20761 gaaattatta gaattacaag ggattgccaa caccacgctg gagatggttg ctgaacgtgt
    20821 ggattatcca ctggacgagc tacgccgctt ctggccagac aaagaggcaa tcctctacga
    20881 tgcgctgcgt tatcttagcc aacagataga tgtctggcgt cgtcagctga tgcttgacga
    20941 aacgcaaacc gccgaacaaa agctgctggc acgttatcag gcgttatcgg agtgcgttaa
    21001 aaacaaccgc tatccgggct gtctatttat cgctgcctgt acgttttatc ccgaccctgg
    21061 ccaccctatt catcaactgg ccgatcagca aaaaagcgcg gcctacgatt tcacccacga
    21121 actgttaacc acgctggaag ttgacgatcc ggcgatggta gcaaagcaga tggaactggt
    21181 gctggaaggc tgtttaagcc gaatgctggt gaatcgtagc caggcggatg tcgacaccgc
    21241 acatcggctg gcggaagata tcctgcgctt cgcccgctgc cgccagggtg gtgcactgac
    21301 ctgacagaaa cacagaaaag aagcgatttg ccgcaatctt aagcagttga atcgctttta
    21361 ctgaaattag gttgacgaga tgtgcagatt acggtttaat gcgccccgtt gcccggatag
    21421 ctcagtcggt ag
//
LOCUS       DAZPLI010000494         6808 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.guided.193, whole genome shotgun sequence.
ACCESSION   DAZPLI010000494 DAZPLI010000000
VERSION     DAZPLI010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.guided.193"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            <1..837
                     /locus_tag="ACHUU3_004708"
     CDS             <1..837
                     /locus_tag="ACHUU3_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFR2084530.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(944..1348)
                     /locus_tag="ACHUU3_004709"
                     /pseudo
     CDS             complement(944..1348)
                     /locus_tag="ACHUU3_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(1392..1781)
                     /locus_tag="ACHUU3_004710"
     CDS             complement(1392..1781)
                     /locus_tag="ACHUU3_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084531.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            1999..2439
                     /locus_tag="ACHUU3_004711"
     CDS             1999..2439
                     /locus_tag="ACHUU3_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR2084532.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(3184..3333)
                     /locus_tag="ACHUU3_004712"
     CDS             complement(3184..3333)
                     /locus_tag="ACHUU3_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR2084533.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            3904..4062
                     /locus_tag="ACHUU3_004713"
     CDS             3904..4062
                     /locus_tag="ACHUU3_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR2084534.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
     gene            4522..5337
                     /gene="sul2"
                     /locus_tag="ACHUU3_004714"
     CDS             4522..5337
                     /gene="sul2"
                     /locus_tag="ACHUU3_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR2084535.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            5398..5925
                     /locus_tag="ACHUU3_004715"
                     /pseudo
     CDS             5398..5925
                     /locus_tag="ACHUU3_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            5946..6419
                     /gene="dfrA14"
                     /locus_tag="ACHUU3_004716"
     CDS             5946..6419
                     /gene="dfrA14"
                     /locus_tag="ACHUU3_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HFR2084536.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            6494..6769
                     /locus_tag="ACHUU3_004717"
                     /pseudo
     CDS             6494..6769
                     /locus_tag="ACHUU3_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
BASE COUNT         1670 a         1782 c         1761 g         1595 t
ORIGIN      
        1 atgttcatgc cgcctgtttt tcctgctcat tggcacgttt cgcaacctgt tctcattgcg
       61 gacacctttt ccagcctcgt ttggaaagtt tcattgccag acgggactcc tgcaatcgtc
      121 aagggattga aacctataga agacattgct gatgaactgc gcggggccga ctatctggta
      181 tggcgcaatg ggaggggagc agtccggttg ctcggtcgtg agaacaatct gatgttgctc
      241 gaatatgccg gggagcgaat gctctctcac atcgttgccg agcacggcga ctaccaggcg
      301 accgaaattg cagcggaact aatggcgaag ctgtatgccg catctgagga acccctgcct
      361 tctgcccttc tcccgatccg ggatcgcttt gcagctttgt ttcagcgggc gcgcgatgat
      421 caaaacgcag gttgtcaaac tgactacgtc cacgcggcga ttatagccga tcaaatgatg
      481 agcaatgcct cggaactgcg tgggctacat ggcgatctgc atcatgaaaa catcatgttc
      541 tccagtcgcg gctggctggt gatagatccc gtcggtctgg tcggtgaagt gggctttggc
      601 gccgccaata tgttctacga tccggctgac agagacgacc tttgtctcga tcctagacgc
      661 attgcacaga tggcggacgc attctctcgt gcgctggacg tcgatccgcg tcgcctgctc
      721 gaccaggcgt acgcttatgg gtgcctttcc gcagcttgga acgcggatgg agaagaggag
      781 caacgcgatc tagctatcgc ggccgcgatc aagcaggtgc gacagacgtc atactagata
      841 tcaagcgact tctcctatcc cctgggaaca catcaatctc accggagaat atcgctggcc
      901 aaagccttag cgtaggattc cgccccttcc cgcaaacgac cccaaacagg aaacgcagct
      961 gaaacgggaa gctcaacacc cactgacgca tgggttgttc aggcagtact tcatcaacca
     1021 gcaaggcggc actttcggcc atccgccgcg ccccacagct cgggcagaaa ccgcgacgct
     1081 tacagctgaa agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc
     1141 catgctccag ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca tagcccggca
     1201 attcctttcc ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct
     1261 gatagagaag ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc
     1321 tggccgtcct ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata
     1381 cagtctatcg cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga
     1441 cattgccagc cagtgcccgt cactcccact cttacattgt tcgcagtatc taggtacatg
     1501 ttattgcgtg caccttcatg atgtataatg ttattaatga cagcagaaag atcgacagaa
     1561 ttaatattct tcgtacctgg gacagttatc gggtgtcggc cagtgatgag accaaaggat
     1621 gcagtataag atgaaccact actattatca tggccaccat taccaccacc gttaaaacta
     1681 cctcccgaac ttccagagcc gctatgacca tcattgtagg ggtcatttgg tcccatgtga
     1741 ccaaaaccac cgacagtacc gctatttaac tctgtactca taaaattatc ctctttgact
     1801 tttaaaaaca gcaaaaactg tatttataac aatgtgttta tatacagtat tcgaaatact
     1861 acgcccgtca aaaccacacc gtcaacaggt tttttgtgta tttttcacgc ctcacgggca
     1921 gcctcagacc ttttgtgtta aaacagtaat atgttctgct gttacaatac ggtacacaca
     1981 ggagacactg tcacttttat ggcatggcac acagttcaag agacactgaa attaacagga
     2041 aagtcgcgct cgcaattata tcgtgacatg gctaaaggcc ttgtcagcta tcgcacagga
     2101 aaaaatgatc gccgggagtt cgagaccagt gaactgattc gggcctatgg cgagctgcgc
     2161 acttctgaga caccaaaaag gcacaaggag ggacactcca aatctgtcgt tagccaggcg
     2221 gaaaatgctc agctagacgt cattcagcag aaactggacg ctctgcagca gactgttacc
     2281 ctaatgcttg aagacaagat ggcgcgggaa gcagaaaacc gcctgcgaaa tgaggagagg
     2341 gaacaactac aggccgaaat tagccgtctg gctgacgcgc tggcacagga aaaaaagcgg
     2401 ggattctggt cgcggctgtt tcgctccggt tcagagtaac agtgcaggtg actctgattt
     2461 accataacat ctgttatcag caccgcctga gaggccctca gggcgtttct gacgggaaac
     2521 cggtaaaaca ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta
     2581 cggcatgcat gagtatgcgc acaagtgcat agcgcggaaa ttcacaccat tcagccgggg
     2641 taagtcctgt ttccggcgtt cttcccatcc gaaaacggtg aacggggacg caatttattg
     2701 cgtggccgtg aagcgttgcc ggcgcagccg gaaaacccgc tgtcagcacg ttcaggaagg
     2761 gggtgatgta caaatggcac agaggatgaa agtgacgtat gagctgaagg aatgatgtaa
     2821 aagtgatgta taagtgatgt aaaagtgatg tatgaactga aggagcgatg taattctgta
     2881 gtcaggaaga ataacccgtt catgcaggca tgaacgatac agtgaggaac gctttttttg
     2941 tcacgatctc cccttaacct gcgtaaatca cccgccatgg ttgattattt ttcaacctca
     3001 aaatggtcat acacctaacg cagccgcgat tttcatcgca actgcatgaa ttaaaagaaa
     3061 aaaaaggaat atttcgtttt cacgagtaat acccgggtat taaaaatcca ctgtaattac
     3121 atgaaaataa agaaataaaa ggtaataccg gagtattacc acgtacgcgg gcaagcgaaa
     3181 aagctataac gcttcccggc cgacaggcga cccaaaatcc acaggggcat gcaggttctc
     3241 ttcagtaacc tgcgccatta aatcaaaaag gtcatattcg ccgggaagct gcgtatcagt
     3301 cgaatccagt tgttcatcat attcagatgg catggggtga tatccagtag ctgagaggtt
     3361 gttagcgggt ttcggggcgc agccctgaac cagtcatgta gcgctagcgg agtgtatact
     3421 ggcttactat gttgccacga ttgcgaaaat cagtgaagtg cttcatgtgg caggagaaaa
     3481 aaggcagcac cggcgcgtca gcagaatatg cgatacagga gatattccgc ttcctcgctc
     3541 actgactcgc tacgctcggt cgttcggctg cggcgagcgg taccggctta cggacgggga
     3601 cggaaatttc ctggaagata ccaggaagac acttagcaaa gaagagataa ggccacggcg
     3661 aagccgtttt tccataggct ccgcccccct gacaagcatc gcaaaatctg acgctcaaat
     3721 cagtggtggc gaaacccgac aggactataa agatcccagg cgtttccccc tggtagctcc
     3781 ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg
     3841 cggtttctca ttccacgcct gacactcagt tccgtgaagg cagttcgctc caagctggac
     3901 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tgtccggtaa ctatcaactt
     3961 gagtccaacc cggaaagaca cgacaaaacg ccactggcag cagccattag taacaggagt
     4021 gcacgagaag atagattaag aggtcttgaa gtagaggcct gactgcggct acactgaaag
     4081 aacagttttg gtaatggcgg tctcgtgaac cctgttaccg ggttaagcag ttccccaact
     4141 gacttaacct tcgaacaaac cgcctcccca ggcggttttt tcgtttacag agcaagagat
     4201 tacgattcat tgaaaaggat ctcaagaaga tcctttgtag attactatca attaatcttc
     4261 cgaacatgac accagtcact gttgattacg gttaaaccgc tccgtaacag gtttccctcg
     4321 tattggtgac ttgcagcacc aaagaagcaa aaaagataaa tatatctact tattgagaga
     4381 tgatatattt atttccaaaa tgctacagat acatcatcat gaaacggatc accgcaaaca
     4441 ggttactcgc ctggggattc cctttcgacc cgagcatccg tatgagactc atgctcgatt
     4501 attattatta tagaagcccc catgaataaa tcgctcatca ttttcggcat cgtcaacata
     4561 acctcggaca gtttctccga tggaggccgg tatctggcgc cagacgcagc cattgcgcag
     4621 gcgcgtaagc tgatggccga gggggcagat gtgatcgacc tcggtccggc atccagcaat
     4681 cccgacgccg cgcctgtttc gtccgacaca gaaatcgcgc gtatcgcgcc ggtgctggac
     4741 gcgctcaagg cagatggcat tcccgtctcg ctcgacagtt atcaacccgc gacgcaagcc
     4801 tatgccttgt cgcgtggtgt ggcctatctc aatgatattc gcggttttcc agacgctgcg
     4861 ttctatccgc aattggcgaa atcatctgcc aaactcgtcg ttatgcattc ggtgcaagac
     4921 gggcaggcag atcggcgcga ggcacccgct ggcgacatca tggatcacat tgcggcgttc
     4981 tttgacgcgc gcatcgcggc gctgacgggt gccggtatca aacgcaaccg ccttgtcctt
     5041 gatcccggca tggggttttt tctgggggct gctcccgaaa cctcgctctc ggtgctggcg
     5101 cggttcgatg aattgcggct gcgcttcgat ttgccggtgc ttctgtctgt ttcgcgcaaa
     5161 tcctttctgc gcgcgctcac aggccgtggt ccgggggatg tcggggccgc gacactcgct
     5221 gcagagcttg ccgccgccgc aggtggagct gacttcatcc gcacacacga gccgcgcccc
     5281 ttgcgcgacg ggctggcggt attggcggcg ctgaaagaaa ccgcaagaat tcgttaactg
     5341 cacattcggg atatttctct atattcgcgc ttcatcagaa aactgaagga acctccattg
     5401 aatcgaacta atattttttt tggtgaatcg cattctgact ggttgcctgt cagaggcgga
     5461 gaatctggtg attttgtttt tcgacgtggt gacgggcatg ccttcgcgaa aatcgcacct
     5521 gcttcccgcc gcggtgagct cgctggagag cgtgaccgcc tcatttggct caaaggtcga
     5581 ggtgtggctt gccccgaggt catcaactgg caggaggaac aggagggtgc atgcttggtg
     5641 ataacggcaa ttccgggagt accggcggct gatctgtctg gagcggattt gctcaaagcg
     5701 tggccgtcaa tggggcagca acttggcgct gttcacagcc tatcggttga tcaatgtccg
     5761 tttgagcgca ggctgtcgcg aatgttcgga cgcgccgttg atgtggtgtc ccgcaatgcc
     5821 gtcaatcccg acttcttacc ggacgaggac aagagtacgc cgcagctcga tcttttggct
     5881 cgtgtcgaac gagagctacc ggtgcggctc gaccaagagc gcaccgttaa cccaggatga
     5941 gaaccttgaa agtatcattg atagctgcga aagcgaaaaa cggcgtgatt ggttgcggtc
     6001 cagacatacc ctggtccgcg aaaggggagc agctactttt taaagcattg acctacaatc
     6061 agtggcttct ggtgggtcgc aagacgtttg aatctatggg cgcactcccc aataggaaat
     6121 acgcggtcgt tacccgctca ggttggacat caaatgatga caatgtagtt gtatttcagt
     6181 caatcgaaga ggccatggac aggctagctg aattcaccgg tcacgttata gtgtctggtg
     6241 gcggagaaat ttaccgagaa acattaccca tggcctctac gctccactta tcgacgatcg
     6301 acatcgagcc agagggggat gttttcttcc cgagtattcc aaataccttc gaagttgttt
     6361 ttgagcaaca ctttacttca aacattaact attgctatca aatttggaaa aagggttaac
     6421 aaagctatgc aatcgacggc aaaaagcttc gttcgcttcg cgcactacgc ctttttccgc
     6481 gattgatagc gacgatatgg ttgtttgcca tggtgatccc tgcatgccga acttcatggt
     6541 ggaccctaaa actcttcaat gcacgggtct gatcgacctt gggcggctcg gaacagcaga
     6601 tcgctatgcc gatttggcac tcatgattgc taacgccgaa gagaactggg cagcgccaga
     6661 tgaagcagag cgcgccttcg ctgtcctatt caatgtattg gggatcgaag cccccgaccg
     6721 cgaacgcctt gccttctatc tgcgattgga ccctctgact tggggttgat gttcatgccg
     6781 cctgtttttc ctgctcattg gcacgttt
//
LOCUS       DAZPLI010000495         4819 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1508941
            SAMN43276767-rid23487413.guided.232, whole genome shotgun sequence.
ACCESSION   DAZPLI010000495 DAZPLI010000000
VERSION     DAZPLI010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43276767
            Sequence Read Archive: SRR30421474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396134.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396134.1
            Genome Coverage       :: 122x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 126 of 288
            Pseudo Genes (incomplete)         :: 107 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 30 of 288
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43276767-rid23487413.guided.232"
                     /strain="M1508941"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2015-09-15"
                     /collected_by="FSS"
     gene            1..652
                     /locus_tag="ACHUU3_004718"
                     /pseudo
     CDS             1..652
                     /locus_tag="ACHUU3_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            649..1263
                     /gene="prfH"
                     /locus_tag="ACHUU3_004719"
     CDS             649..1263
                     /gene="prfH"
                     /locus_tag="ACHUU3_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR2084537.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHUU3_004720"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHUU3_004720"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR2084538.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3038..3496
                     /gene="gpt"
                     /locus_tag="ACHUU3_004721"
     CDS             3038..3496
                     /gene="gpt"
                     /locus_tag="ACHUU3_004721"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR2084539.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3588..>4819
                     /gene="frsA"
                     /locus_tag="ACHUU3_004722"
     CDS             3588..>4819
                     /gene="frsA"
                     /locus_tag="ACHUU3_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR2084540.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIE"
BASE COUNT         1145 a         1207 c         1289 g         1178 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac agcggcatat
       61 tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc cagctgatct ctttacgcat
      181 aatgcaacag gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcact cggtgattac tcctagcttg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggct gggcgtaaat gggggcgcac
      421 cgagtgtaaa gggcgtctgg cagcgaaata cacagcgacg cagctctcac gtactgaact
      481 tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggct ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggag
      721 cgattaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg cggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
      961 caatcggatg caatccgtta tgagacgttg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gcgttttgtt gaacagacgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcaga
     1681 gaacggatca gacagtgaat ttctacgtta ttatcagtca tggtcaccac accgacgttc
     1741 agggaggttt cgaccacacc ttttgccacg tctgagttgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc aggacgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcagat ccagttcttc tgcatgaccc gctaggaagc gcaccagcag tttgttggca
     2101 ttacccaggc caacgtggat ttccccgccg gagtgacccc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     2401 accgccagcg cagacgccat accaatgccg ttatctgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccga tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3001 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3061 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3121 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3181 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3241 gcgcgagctt aaagtgctaa aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     3301 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     3361 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     3421 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     3481 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     3541 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3601 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     3661 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     3721 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgtg
     3781 aaatcctcga cgtccaggcg cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     3841 tatacgatac ggtgattggc taccgtggtg gcaactggat ttatgagtgg gccatccagg
     3901 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgtcact
     3961 ggctgcatgc ggctacgttg tacaacattg ctgcctatcc tcatctgaaa ggagatgacc
     4021 tggccgagca agcgcaggct ttgtcaaacc gtgcctatga agaagccgcc cagcgtctgc
     4081 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     4141 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     4201 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcga
     4261 tgctgactat tgatatgccg tcggtgggct tttcatcaaa atggaagctc acccaggact
     4321 ccagcctgtt gcatcagcac gttttaaagg ctctgcctaa cgtaccgtgg gtggatcaca
     4381 ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatatc
     4441 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
     4501 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt
     4561 tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa
     4621 aagtgcaagg attgctgggg cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     4681 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
     4741 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
     4801 tcaccggctg gatcgaaaa
//
